LOCUS       DASQDO010000001         7339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_183627, whole genome shotgun sequence.
ACCESSION   DASQDO010000001 DASQDO010000000
VERSION     DASQDO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183627"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VEV43_00005"
     CDS             <1..621
                     /locus_tag="VEV43_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HYP21935.1"
                     /translation="DRRGEGPVTRPIVRRTHAGSRAARPLKIVVTGPFAAGKTSLIQT
                     ISEVAVIGTERTITDETRERKETTTVAMDFGRVSLGDGLSLLLFGTPGQRRFEVMWEV
                     LSEGMIGFVLLVNGSDERSAAEAARQLETFRAYADVPFVVGVTHLDEPGVDPAETLAR
                     VRERLDVGPEAEVVPCDPRSRDDVKDVMLRILFGVLRRLEPVKAAG"
     gene            706..2412
                     /gene="gspE"
                     /locus_tag="VEV43_00010"
     CDS             706..2412
                     /gene="gspE"
                     /locus_tag="VEV43_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HYP21936.1"
                     /translation="MTVTSKPRAAGLAELLLARRLVTEQQLQEAKDQAAQRQRSLGRV
                     LIELGFVTEAGLVSVLAQQLGLEFVDLTETKVDGSAVALVPELVARRHNCLPIGFDDN
                     GRLVVAMADPANVVAVDDIRAMSKRDVKTVVATKADVAAAIGRHYRLDRTAETLVEEA
                     AAEQEGEAAHDLEAQLATAGAEDAPIIKLVNLLITQAVQDRASDIHIEPGEKDLRVRY
                     RIDGVLHEVMSPPKSVQAGITSRLKIMADINIAERRVPQDGRIGLVLQGKQIDIRVAT
                     LPTVHGEKIVMRILDKSSVLLQLSDLGFLPENFQRFRESYMKPYGAILVTGPTGSGKS
                     TTLYATLNIKNKPDVNIITVEDPVEYRLAGINQVQTNPKAGLTFASALRSILRSDPDI
                     VLIGEIRDRETAQIAVEAALTGHLVLSTLHTNDAPSALTRLVEMGIEPFLVASSLDSV
                     LAQRLARKLCDRCKEPYAPSIDELTHAKFPFDPDQEPPKLYRPVGCTSCGNTGYKGRM
                     AIHELMPVTEDIERLVAENASAEVIGRTAREQGMITLRTDGMEKARMGLTSIEEILRV
                     VV"
     gene            complement(2375..3397)
                     /locus_tag="VEV43_00015"
     CDS             complement(2375..3397)
                     /locus_tag="VEV43_00015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYP21937.1"
                     /translation="MSAAAARSPLVTVVTPCLDPGWRLERCIASVAAQTYAHVEHVVV
                     DGASTDGTVELLRGTPSVKWVSEPDSGQTDAINKGFAMGGGEILTWLNADDELLPDAV
                     EKAVACFADPAIGWSYANTELVYPDKTQTWVAPATLRPDSFDWIMPFPQQGWFIARWA
                     LDRVGPLDETFHLSMDYDLCLRLFTAGIPHCYVPSTLARFEVHAESKTGSTSYVAFID
                     EGARAMRKAGRLTAFYMAMGQRCAYLSAEDDRVDRARLRAEVLSTVADHAGRPRRDRA
                     AVKAGAYTAAAQIELKSERNPKPLRSLGWLLRPEPWRFGVTRGRSMDLAKTAVRRRLG
                     GSPRSR"
     gene            complement(3394..4314)
                     /locus_tag="VEV43_00020"
     CDS             complement(3394..4314)
                     /locus_tag="VEV43_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYP21938.1"
                     /translation="MDPVRQPELAVVVPSRNRSRLLADCVGSLLRQDTKAGYEVLVVD
                     DGSTDDTRERIEALIPGAEGRLRYVHQPPSGLNAARNAGLRDTGAPTVAFLDDDALAP
                     ETYVQAVVEGTASNPGVDCFGGRIRLRLEGEVPRWCGREPIGETELEEGDEDRFVEAA
                     WGANLIVRRSAIERVGPFAEDMALYGDEVEWQSRIRREGGRIVYLAQAWVWHRRTGNE
                     LRLKALLRRNFKKGLSYAQTRILMQRHVSPWGGLRHVALGLGHAARRRCWIGIVRAAL
                     GAGHAIGALRLLTSGTSSPYLHWLPARRES"
     gene            complement(4331..5503)
                     /locus_tag="VEV43_00025"
     CDS             complement(4331..5503)
                     /locus_tag="VEV43_00025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP21939.1"
                     /translation="MRISFLTPEYPPFGTTGGIGSYVSIIAPTLAARGHEVRVITCTG
                     ALREDVVDRGVHVHVRPLRPLPVVGRMDRVSQTHARLAAARSYRAALRELERPDVIES
                     PEWMAESLRIRGSTRRSRLLVHLHTPVGFIARHGQRLGRDAALAHRIELLSIRGARAV
                     TSPSKLLLDLVFPSGAPQGPLARVIRQPIDVDLWKRSDAVSRTERVVLVVGRLERLKG
                     VDVLLEAAGKLPPEVRDTTTIVAVGRSSGLHEGRPYAEWLSKLAERHGVRFEQRGEVA
                     RDELAALYAAARVVALPSRFDNFPMVALEAMASGRPLVCSTTCGAAELVGGSGAGEVF
                     ESGDAGALASALLRYLTDEAAARAAGDTGRALVEAECAPDVIAGQRERLYEDMAEA"
     gene            complement(5527..6759)
                     /locus_tag="VEV43_00030"
     CDS             complement(5527..6759)
                     /locus_tag="VEV43_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HYP21940.1"
                     /translation="MAGLGKRYRIGVERHGTFRDALAHPLRRLKTRVRAGSGAASHVW
                     ALQDVSFEVAEGEAVGIIGRNGAGKSTLLKILARITRPTTGRASLAGAVGSLLEVGTG
                     FHPELTGRENVYLNGAILGMTKGYIDRRFDEIVSFAETDRFVDTPVKRYSSGMQMRLA
                     FAVAAHLEPEILLVDEVLAVGDAQFQRKCLGKMSEVAGEGRTILFVSHNMGSIVRLCD
                     RALWIDSGRLQKDGPARDVVRSYLTVGEAEQTAQTWGPGEGPGDDSLRLASVRITQEG
                     KPTTNVHIDKPFDVEVETEVNAESSGGVAVAIRVLNPEGDILLESSEVMQDDLPVRRR
                     GRWVTKCSFPAYALNGGSYYLSVFADEPHVKVVFSEESVLKWTVEAVSAGMGRYAPGA
                     WKGVLGPGLPQWSLHGVD"
     gene            complement(6780..>7339)
                     /locus_tag="VEV43_00035"
     CDS             complement(6780..>7339)
                     /locus_tag="VEV43_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP21941.1"
                     /translation="WTYFSLAVVTASNSVVGNQNLIHKIYFPRLLLPMSSVLPGVIDF
                     AIAFVGLVVLMLIYGVGFGLPFLLFPVFLLMAVLLALALGVWLSILNARFRDIRFVVP
                     FLVQMLFFASPVVYSADQIPDEWKVLYGLNPLVGIIDGFRWSITGAGEAPGLSVLLSV
                     AFVLVVLVGGLVYFRKEEATIADIV"
BASE COUNT         1170 a         2625 c         2477 g         1067 t
ORIGIN      
        1 gatcgacggg gtgaagggcc tgtgacgagg ccgatcgtcc ggcggacgca cgccggctca
       61 cgggcggccc ggcccctcaa gatcgtggtg accgggccct tcgccgccgg gaagacctcg
      121 ctgatccaga cgatctccga ggtcgccgtg atcgggaccg agcggacgat caccgacgag
      181 acccgggaga ggaaagagac gacgacggtc gcgatggact tcggccgggt gtcgctcggg
      241 gacggtctct cgctgctgct gttcggcacg cccgggcaga ggcgcttcga ggtcatgtgg
      301 gaggtcctct ccgaggggat gatcgggttc gtcctgctgg tgaacggctc cgacgagcgc
      361 tcggcggccg aggcggcgag gcagctcgag acgttccggg cttacgcgga cgtcccgttc
      421 gtcgtgggcg tgacgcacct cgacgagccg ggcgtcgatc cggcggagac gctggcccgg
      481 gtgcgggaac ggctcgatgt ggggcccgag gcggaggtcg tcccctgcga cccccgctcc
      541 cgggacgacg tgaaggacgt gatgctgcgg atcctcttcg gggtgctgcg gcgactggag
      601 cccgtgaagg cggcgggctg accggattgg cccttcagtc ggagggccag ccgccgatat
      661 aggccctatg ggagggccac ggtcgcgacg tcaccagggg agcagatgac ggtcacttcg
      721 aaacctcggg cagccgggct ggccgagctc ttgctcgccc gccgcctggt caccgagcag
      781 cagctacaag aggcgaagga ccaggctgcg cagcgccagc gctcgctcgg ccgcgtcctg
      841 atcgagctcg gcttcgtcac cgaggcgggg ctcgtctccg tcctggcgca gcagctgggg
      901 ctcgagttcg tcgacctcac ggagaccaag gtggacgggt ccgccgtcgc cctggtgccg
      961 gagctcgtcg cccgccgtca caactgcctg ccgatcgggt tcgacgacaa cggccgtctc
     1021 gtagtcgcga tggccgaccc ggccaacgtc gtggcggtcg acgacatccg cgcgatgagc
     1081 aagcgcgacg tcaagaccgt cgtcgccacc aaggccgacg tggccgccgc gatcggccgt
     1141 cactaccgtc tcgaccgcac ggccgagacg ctggtggagg aggccgctgc cgagcaagag
     1201 ggcgaagcgg cccacgacct cgaggcccag ctcgccaccg cgggggccga ggacgccccg
     1261 atcatcaagc tcgtcaacct gctgatcacc caggcggtcc aggaccgcgc ctcggacatc
     1321 cacatcgagc cgggcgagaa ggacctgcgc gtccgctaca ggatcgacgg cgtgctccac
     1381 gaggtcatga gcccgccgaa gtccgtgcag gccgggatca cgagccgcct gaagatcatg
     1441 gcggacatca acatcgcgga gcgccgcgtc cctcaggacg gccgcatcgg cctcgtcctc
     1501 cagggcaagc agatcgacat ccgcgtggct acgctcccga ccgtccacgg cgagaagatc
     1561 gtcatgagga tcctggacaa gtcgagcgtg ctgctccagc tgtccgacct cggcttcctg
     1621 ccggagaact tccagcgctt ccgcgagagc tacatgaagc cgtacggggc gatcctcgtc
     1681 accgggccga cggggtcggg gaagtcgacg acgctctatg cgacgctgaa catcaagaac
     1741 aagccggacg tgaacatcat cacggtcgag gacccggtcg agtaccgcct cgccggcatc
     1801 aaccaggtcc agacgaaccc gaaggcgggc ctcacgttcg cctccgccct gcggtcgatc
     1861 ctgcgatccg accccgacat cgtgctcatc ggtgagatcc gtgaccgcga gacggcacag
     1921 atcgcggtcg aggccgcgct gaccggccac ctcgtgctct cgacgctcca cacgaacgac
     1981 gcgccgtcgg cgctgacccg gctcgtcgag atggggatcg agccgttcct cgtcgcctcg
     2041 tcgctggact cggtactggc gcagcgactc gcccgcaagc tctgcgaccg ctgcaaggag
     2101 ccgtacgcac cgtcgatcga cgagctcacg cacgcgaagt tcccgttcga cccggaccag
     2161 gagcccccga agctctaccg cccggtgggc tgcacctctt gcggcaacac cgggtacaag
     2221 ggccggatgg cgatccacga gctcatgccc gtcaccgagg acatcgagcg cctcgtcgcc
     2281 gagaacgcct cggccgaggt catcggccgc accgcccggg agcaggggat gatcacgctg
     2341 cgcaccgacg ggatggagaa ggcccgcatg ggcctcacct cgatcgagga gatcctccga
     2401 gtcgtcgtct gacggccgtc ttcgcaaggt ccatcgagcg gccgcgggtg acgccgaaac
     2461 gccagggctc cgggcgcagc agccatccga ggctccgcaa cggcttggga ttccgctctg
     2521 acttcaactc gatctgggcc gccgccgtat aggcgccggc tttcacggcc gccctgtccc
     2581 gtcgtggccg gccggcgtga tccgcgacgg tcgatagaac ctccgcgcgc aagcgggccc
     2641 ggtccactcg atcgtcttcc gccgagagat acgcgcaccg ctggcccatg gccatgtaga
     2701 aggcggtcag ccggcccgcc ttcctcatgg cccgggcgcc ttcgtcgatg aacgcgacgt
     2761 agctcgtcga tcccgtcttc gactcggcgt gcacctcgaa gcgcgccagc gtcgacggca
     2821 cgtagcagtg ggggatcccc gccgtgaaca gccgcaggca gaggtcgtag tccatcgaga
     2881 gatggaacgt ctcgtccagc gggccgacgc ggtcgagggc ccagcgtgcg atgaaccagc
     2941 cctgctgcgg gaacggcatg atccagtcga agctgtccgg acggagggtc gccggcgcca
     3001 cccaggtctg ggtcttgtcc ggatagacga gctccgtgtt cgcatacgac cacccgatcg
     3061 cggggtcggc gaagcacgcc acagccttct cgaccgcgtc ggggaggagc tcgtcgtcgg
     3121 cgttcagcca cgtgaggatc tcgcccccac ccatggcgaa gcccttgttg atcgcgtcgg
     3181 tctggcccga gtcgggctcg gagacccact tcacggacgg cgtcccccga agcagctcga
     3241 cggtcccgtc ggtcgacgcc ccgtcgacca cgacgtgctc gacgtgcgcg taggtctggg
     3301 cggcgacgct cgcgatgcat cgttcgagcc gccaccccgg gtcgaggcag ggggtgacga
     3361 cggtcacgag cggggaccgg gccgcggcgg cgctcacgat tcgcgccgcg ccggcagcca
     3421 gtgcaggtac ggcgacgacg tgccggacgt cagcagcctc agcgcgccga tcgcgtggcc
     3481 ggcccccagg gccgcacgca cgatgccgat ccagcaccgg cggcgggccg cgtgtccgag
     3541 gcccaaggcg acgtgtctca ggcctcccca cggcgagacg tgccgttgca tgaggatccg
     3601 cgtctgcgcg tagctgagcc ccttcttgaa gttcctgcgg aggagcgcct tcagccggag
     3661 ctcgtttccc gttcgccggt gccagaccca cgcctgcgcg aggtacacga tccgtccgcc
     3721 ctcgcggcgt atccggctct gccattcgac ctcgtcgccg tagagggcca tgtcctccgc
     3781 gaacgggccg accctctcga tcgcgctgcg tcgaacgatc aggttcgctc cccacgcggc
     3841 ttcgacgaac cggtcctcgt cgccttcctc cagctcggtc tcgccgatgg gctcgcgtcc
     3901 gcaccagcgc gggacctctc cctccagccg gagccttatc cggcccccga agcagtccac
     3961 acccgggttc gaggcggtgc cctcgacgac cgcctgcacg taggtctcgg gggcgagggc
     4021 gtcgtcgtcg aggaacgcga ccgtcggggc gccggtgtcc cggagcccgg cgttgcgcgc
     4081 ggcgttcaac ccgctcggag gctggtgcac gtatcgcagt cgcccttccg cgccggggat
     4141 gagtgcttcg atccgctcgc gcgtgtcgtc ggtcgagccg tcgtcgacga cgaggacctc
     4201 gtagcccgcc ttcgtgtcct gccgcagcag gctgccgacg cagtccgcca gcaaccgcga
     4261 ccggttccgg ctggggacca cgaccgcgag ctccggctgt cgtacgggat ccaaagtcga
     4321 cgggcctcct ctacgcctcg gccatgtctt cgtacagccg ttccctctga cctgcgatca
     4381 cgtccggcgc gcactccgcc tcgacgagcg ccctgccggt gtcgccggcc gctcgggccg
     4441 cggcttcgtc cgtcaggtaa cgcagcagcg cggacgccag cgcgccggcg tcgccgctct
     4501 cgaagacctc accggcgccg cttccaccga ccagttccgc cgctccgcaa gtcgtcgagc
     4561 acacgagcgg cctccccgaa gccatcgcct ccaacgccac catcgggaag ttgtcgaagc
     4621 ggctgggcag cgcgacgacg cgggccgcgg catagagcgc ggcgagctcg tctcgcgcca
     4681 cctcgccgcg ctgctcgaac cgcacgccgt gtcgctcggc gagcttcgac agccactccg
     4741 cgtagggccg gccctcgtgc aggcccgagg acctcccgac cgcgacgatc gtcgtcgtgt
     4801 cgcgcacctc ggggggcagc ttgccggccg cttccagcaa tacgtccacg cccttcagcc
     4861 tctcgagccg cccgacgacg aggacgacgc gttcggtccg ggagacggcg tcggagcgct
     4921 tccagaggtc gacgtcgatc ggctgccgga tcacccgcgc gagagggccc tgaggggccc
     4981 cggacgggaa cacgaggtcg agcagcaact tcgagggcga cgtcaccgcg cgggcccccc
     5041 ggatgctgag caactcgatg cggtgggcca gcgcggcgtc gcgtccgagc ctttgcccgt
     5101 ggcgggcgat gaaccccacc ggcgtgtgga ggtggacgag gagccgggag cggcgcgtcg
     5161 acccgcggat ccgcaacgac tcggccatcc actccggcga ctcgatcacg tccggtcgtt
     5221 cgagctctcg cagcgcggcc cggtacgaac gcgccgcggc aagacgcgcg tgcgtctgcg
     5281 agacgcgatc catccggccg accaccggca ggggccgcag cggcctgacg tgcacgtgca
     5341 cgcctctgtc gacgacgtcc tcgcggagtg ctccggtgca cgtgatcacg cggacctcgt
     5401 ggccccgcgc cgcgagcgtc ggggcgatga tcgagacgta cgagccgatc cctcccgtcg
     5461 ttccgaaggg cggatattcc ggagtcagga aagagatcct catcggggcg actcttcgct
     5521 gccggctcag tcgaccccgt gcagagacca ctggggcagg cccgggccga gcactccctt
     5581 ccaggcgccg ggcgcgtagc ggcccatgcc cgcggacaca gcttcgacgg tccatttcag
     5641 aacgctctcc tccgagaaca cgaccttgac gtggggctcg tctgcgaaga ccgagagata
     5701 gtaggagcct ccgttgaggg cgtaggccgg gaacgagcac ttggtgaccc agcgaccgcg
     5761 ccggcggacg ggcaggtcgt cctgcatcac ctcggagctc tccagcagga tgtcgccttc
     5821 gggattgagc acgcggatcg cgaccgcgac gcctcccgac gactcggcgt tcacctcggt
     5881 ctccacctcg acgtcgaaag gcttgtcgat gtggacgttc gtcgtgggtt tgccttcctg
     5941 cgtgatgcgc acgctcgcca ggcgcaggga gtcgtcgccc gggccctcac ccggccccca
     6001 cgtctgtgcg gtctgctcgg cttcacctac ggtgagatag ctgcggacga cgtcgcgcgc
     6061 cggtccgtct ttctgcaggc gccccgaatc gatccacagc gcgcggtcgc agagccggac
     6121 gatgctcccc atgttgtggc tcacgaacag gatcgtcctg ccctcgcccg cgacctcgct
     6181 catcttcccg aggcacttcc tctggaactg cgcgtcgccg accgcgagca cctcgtcgac
     6241 caggaggatc tcgggctcga gatgcgcggc gaccgcgaac gccagcctca tctgcatgcc
     6301 gctcgagtag cgcttcacgg gagtgtccac gaaccggtcc gtctcggcga acgagacgat
     6361 ctcgtcgaag cgccggtcga tgtagccctt cgtcatgccg aggatggccc cgttcagata
     6421 cacgttctcg cgcccggtca gctcgggatg gaagccggtc ccgacctcga gcaggctccc
     6481 cacggccccc gcgagagagg ccctgcccgt cgtgggcctc gtgatccgcg ccaggatctt
     6541 gagcagcgtg ctcttcccgg cgccgttgcg cccgatgatc cccacggcct ctccctcggc
     6601 cacctcgaag gacacgtcct ggagcgccca cacgtggctc gccgcgcccg acccggcgcg
     6661 gacgcgcgtt ttgaggcgcc gaagcggatg cgccagcgcg tcgcggaacg tgccgtggcg
     6721 ttcgaccccg atccggtacc tcttccccag accggccacg gcgagggcct tggaccgact
     6781 catacgatgt ccgcgatcgt cgcttcctcc ttccggaagt acacgaggcc gccgacgagg
     6841 acgacgagga cgaaagcgac cgacagaagc accgagagtc ccggtgcttc tcccgccccg
     6901 gtgatcgacc accggaaccc gtcgatgatc ccgacgaggg ggttgaggcc gtagagcacc
     6961 ttccactcgt ccgggatctg gtcggcggag tacacgaccg gcgacgcgaa gaacagcatc
     7021 tgcacgagga acgggacgac gaagcggatg tcgcggaagc gcgcgttgag tatcgacagc
     7081 cacaccccca gcgcgagcgc gagcagcacg gccatgagca gaaagaccgg gaacaacagg
     7141 aagggaaggc cgaacccgac cccgtagatc agcatcagga cgacgaggcc cacgaacgcg
     7201 atggcgaagt cgatcacccc cggcaggacc gacgacatcg gcagcagcag cctcgggaag
     7261 taaatcttgt ggatgaggtt ctggttgccg acgacggagt tgctcgccgt gacgaccgcg
     7321 agcgagaagt acgtccacg
//
LOCUS       DASQDO010000002        11323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_918039, whole genome shotgun sequence.
ACCESSION   DASQDO010000002 DASQDO010000000
VERSION     DASQDO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918039"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEV43_00040"
     CDS             <1..448
                     /locus_tag="VEV43_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYP21942.1"
                     /translation="SGSADLVTSAQALHHVHEPAAVGKEMRRVAKPGGRVLLVDQVAT
                     ERYEEALMMTELELARDPSHAMSRPPSAYRMIARSAGLEIVDERVTESEQRLSTWMWP
                     GEFPEERIAAVSDLIEKIGAETGMGFERDGDDWVFTRRRLMLLAER"
     gene            448..2445
                     /gene="ligA"
                     /locus_tag="VEV43_00045"
     CDS             448..2445
                     /gene="ligA"
                     /locus_tag="VEV43_00045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYP21943.1"
                     /translation="MAAKKDAAARVAELREAIDYHSYRYHVLDDPEIADVEYDALVRE
                     LIEIEEAHPDLVTPDSPTQRIGAPPSDLFAPVVHRTPMMSLDNCFSLEELQAWGKRVE
                     RGIGAADGFMVEQKMDGVAVNLIYEGGTLVRGATRGDGRTGEDITSNLKTIAAVPLKL
                     RGDAPDVLEVRGEVYMTTADFEKLNARLGEAEGKVFANPRNAAAGSLRQKDPKVTAQR
                     KLSLICHGIGYAEGRRFRAHSEALETLRELGLRTNANNRRVDDLDRVYEYCTQWQEHR
                     HDVPYEIDGVVVKVDSIDQQEELGFTAKAPRWAIAYKFPPEERTTLLRKISVHVGRTG
                     VATPWAQLEPVFVGGVTISTATLHNEDEVARKDVREGDTVIVRRAGDVIPEVVGPVPS
                     LRRKGAKPWVMPKKCPSCGEPLVRQEGEAATRCVNLECPSQLIETLFHFSSRGAMDIE
                     GLGYKTIIWLIERGLLKDVADVYQLKPADFEGHEGWGEISIRNLTEAIEASKKRPLGN
                     VLIALGIPHVGGRAFDIAAQAGSLDRLKEMTEEDFLAMEGIGPVIARSITQFFAQDRN
                     LDVIRRLQEAGVDPVEVARKKEGPLVGKTFVITGTLEGFSRQGAQDAVEDLGGKVTSS
                     VSKKTDYVVVGANPGASKYDKAKELGIELLDEAAFTELLGR"
     gene            2445..2918
                     /locus_tag="VEV43_00050"
     CDS             2445..2918
                     /locus_tag="VEV43_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21944.1"
                     /translation="MLGWAIALSLTALAVVAFVLAHRKGTRRFEAELSRIVRGSAAEF
                     RATGMLPERPRPPRPRVVVRDDAVTVKVRGRRAQRLEWSRLAEVAVRTTPNGPWQEDV
                     FFLLGDDAGSGIVVPHLEAADLLPYLQRLPGFDNGALIEAMGSAEDANFVCWRRG"
     gene            2960..4141
                     /locus_tag="VEV43_00055"
     CDS             2960..4141
                     /locus_tag="VEV43_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP21945.1"
                     /translation="MGKKSAVARAVTAVILFGALLPGAGAPARARAQGYENKCGGSDV
                     TIAGTSGDDRLRGTPGRDVIAAFGGNDVVFGLGGNDSICDGEGDDRVHAGAGRDSIET
                     VAGMDTVFGERGGDFLYAFAIDAGDSTDSFYGGPGDDILGDDYGDDLTDGGEGVDTYR
                     ALGHAATVIDLTQGYAAVASRGEVNTLVAIENAVGTFYSDQLIGDDGPNVLAGGMGPD
                     NVRGEGGSDLLLSELDGDQLDGGSDPTRDGVMAAVQGPIAADLVAGTLARRGVTSPQD
                     RLVGIEDVVGTAEDDLLVGNGEVNRLFGGPGIDSLDGGAGADVLFGDMPPVWWINPHH
                     WSIPYYPTTAGNDTLEGGEGHDRLNGGARRDLCTGGEVVKRCEDSGDAGARPDVSYSL
                     R"
     gene            4577..5509
                     /locus_tag="VEV43_00060"
     CDS             4577..5509
                     /locus_tag="VEV43_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP21946.1"
                     /translation="MPSLDGKVAVVTGAGRGIGREHALALAGAGAKVVVNDLGGSAAG
                     EGQDQTPAQQVADEIKANGGEATANYDNVADFAGAERMVKQAVADFGRLDILVNNAGI
                     LRDRMLVSMSEAEWDAVIEVHLKGHFAPTHHAAAYWRAQSKEGNQINGRVINTSSPSG
                     VFGNVGQTNYGAAKAGIAAFTVIAAQELGRYGVTVNCLAPNARTRMTEDIFGMDKPAD
                     GFDPLDPSNISPVVVALCTDEAQGITRQCFFIYGGTINVLKPWDLGTALHKEDGWTPD
                     ELASALTEKFPSGIAPEGMMSMLSKAIGGGQPLA"
     gene            5502..5951
                     /locus_tag="VEV43_00065"
     CDS             5502..5951
                     /locus_tag="VEV43_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HYP21947.1"
                     /translation="MPEDVIEFELPVERGKVREFALSVGEDNPIHLDPEAARLHGFPD
                     IVAPPTFTVTQIWQVPREEREERLGANLDYGRVLHGEQEFTYKRLPFAGEVLKGRMRI
                     SKDFTKEGRRGGSMRFVTYESVFTDAEDEEVLTAYYTLIETAKDPGS"
     gene            5948..6400
                     /locus_tag="VEV43_00070"
     CDS             5948..6400
                     /locus_tag="VEV43_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYP21948.1"
                     /translation="MSAPQTITMEGVSPGMEFGEMVFGPLERVDIVKYAGASGDFNPI
                     HTDEGYAQATGAPTVFAMGMLPAGYLAHAAADWFGGSQNLRRFKVRFTTRVWPGDEIA
                     CTGRVVSIEDGLVKVTMEARRRGPGPDGLDLPEEETALIGEADVELPH"
     gene            6423..7271
                     /locus_tag="VEV43_00075"
     CDS             6423..7271
                     /locus_tag="VEV43_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYP21949.1"
                     /translation="MGLNTELVGKEYPASTFEVTADHIERYARATNDENERYLGGDDV
                     VAPPVFPVVPAFNTFMTAAMDPELGADLLRLVHAAEEHVFYKPIRAGDVLTVAGAIES
                     VEQKETGETFTVKGTETNQDGEVVAEVRGTMFIRGSGSGSRSATPPAENARDVVYEET
                     TKVDDDQTFRYAEASGDHNPIHVDEATAKMAGLPGIILHGMCTMAMASKGAVNGLAGG
                     DPTRLARVGVRLSKPVLPGQELTTRFWKESSASHPPSGIATYGFETYNPDGLAVIKNG
                     IVEVRA"
     gene            complement(7268..8161)
                     /locus_tag="VEV43_00080"
     CDS             complement(7268..8161)
                     /locus_tag="VEV43_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYP21950.1"
                     /translation="MNPPEGTQTAEENVETYCYGHPDRPTKLQCTRCDRYICGQCAIQ
                     ASVGQHCPECVAEARKSAPKVRTAMAVNAPVTRAIIALTVAVYVVELFGGNEFVNRFT
                     SLAPAIADGEVYRLLTSVFLHLPLSEGGYAIMHIVFNMYILSIYGAQVEQLFGSARYA
                     FLYLATGFFASAASYGLQQCSNGLGASGAVFGIVGVLMVYLYNRRSSRVVDPHLQSLW
                     LFVLINLALGFTIARIDNIAHIGGLVSGAALAFGFDRGRLSEPKAPAPLQIATAVVVL
                     VIGAGLVVQRTGMFELADCFR"
     gene            8203..8856
                     /locus_tag="VEV43_00085"
     CDS             8203..8856
                     /locus_tag="VEV43_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYP21951.1"
                     /translation="MRVATFNVHHCEGRDGAVDVARVADVIRATGAEVVALQELDMGL
                     ERTGFTNQPGELAAATGLTVEFHSLLQRGDGRYGIALASKEQRLDSELIHLPQQAREE
                     PRGAVVARRGGITFVGTHLSRSRRARAAQIEALAVLAGRVEPPVVLMGDLNQAASGLG
                     PLLAAGFRGPPRRWWTRPPRSIDHILAGPGLRVASQQILRTKASDHPALAAEIVREE"
     gene            8961..9362
                     /locus_tag="VEV43_00090"
     CDS             8961..9362
                     /locus_tag="VEV43_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21952.1"
                     /translation="MTRAAALVLAAGAFAGCARAEALDPAAKERLDEANATASEAVEA
                     VDALEARIDELEAQLDDAERLGDRVDQVSERLWESLAKLRKSLDDVRGGASDAAATAD
                     AAAGQASQAARDLAVLGNRYDYHLRRYHGGG"
     gene            9363..11192
                     /locus_tag="VEV43_00095"
     CDS             9363..11192
                     /locus_tag="VEV43_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYP21953.1"
                     /translation="MRLMRDLVGETLSDRYRLVARIAGGGMGEVYRGQDVLLERPVAV
                     KILQPSLAADPDLVERFKDEARAAARLTHPNIVSVHDWGAADEQTYYMVMEFVAGTDL
                     RDLLVSRGSLAPAQAAEIMACVCDALAAAHAAGVVHRDVKPENVLIARNGRVKVADFG
                     IAAVADADRTMPGGGIPGTLRYLSPEQASGRAASPASDVWAAGAVLSELLTGRPPQQG
                     SGADLLRRRAIEPPVAPSTFDQTLPQALDEIVMKACALDPPERFDDAAEMAAALRRAS
                     VRSLPDAPPVDSLLDDVTGVIRLPDDDKREITRSKRRRRGRRIKVGAALVVLALLAFG
                     AAKAVALLAAPARVDVPTLTGLPRADARAEAEEAGLELEIVGRDTDLDVPKGHVLSQD
                     PADGTLLEGKAIAVVISGGLPKHEVPDLIGMKVDAATTRANVYGFEIVVTGEEFALTD
                     PGEIIEQTPADGKLERGGKLEVVVSKGPQSIGVPNVEGLPATKAQKMLEREGFEVVAV
                     PVYSNTVDLGIVVYTTPPGGSTAPEGSRVDMAVSQGPKYDEVTMPDVRGMTIDAATSE
                     LEGLGLRVNVVQSCGGGGTIVSETQPLGGTKIRENQLVDVFTC"
BASE COUNT         1878 a         3809 c         4095 g         1541 t
ORIGIN      
        1 atccggctcg gccgacctcg tgacgagcgc gcaggccctc caccacgtgc acgagccggc
       61 cgcagtcggg aaggagatgc ggcgcgtggc caagccgggc gggcgcgtgc tcctcgtcga
      121 ccaggtcgcg acagagaggt acgaggaagc gctgatgatg accgagctcg agctcgcccg
      181 cgacccctcg cacgcgatgt cgcggccccc gtccgcctac cggatgatcg cccgctcggc
      241 cgggctcgag atcgtcgacg aacgcgtcac ggagtccgag cagcggctgt cgacgtggat
      301 gtggccgggg gagttcccgg aggagcgcat cgccgcagta agtgacctca tcgagaagat
      361 cggtgccgag accggcatgg ggttcgagcg cgacggggac gactgggtct tcacgcgccg
      421 gcgcctcatg ctgctggcgg agcggtaatg gctgcgaaga aggacgcggc ggcgcgcgta
      481 gccgaattgc gggaggcgat cgactaccac tcgtaccgct accacgtgct cgacgacccc
      541 gagatcgcag acgtcgagta cgacgcgctc gtgcgcgagc tcatcgagat cgaagaagcg
      601 catcccgacc tcgtcacgcc ggactcgccg acgcagcgga tcggcgcgcc gccgtccgac
      661 ctcttcgccc cggtggtcca ccgcacgccg atgatgtcgc tcgacaactg cttctcgctc
      721 gaggagctcc aggcctgggg caagcgcgtc gagcggggga tcggtgccgc ggacggcttc
      781 atggtcgagc agaagatgga cggcgtcgcc gtcaacctga tctacgaggg cgggacgctc
      841 gtgcgcggag cgactcgcgg cgacgggcgc accggcgagg acatcacgtc gaacctcaag
      901 acgatcgcgg ccgtcccgct gaagctccgc ggcgacgctc cggacgtcct cgaggtgcgc
      961 ggcgaggtct acatgacgac cgcggacttc gagaagttga acgctcgtct gggagaagca
     1021 gaagggaagg tcttcgccaa cccccgcaac gccgccgcgg ggtcgctgcg ccagaaggat
     1081 ccgaaggtga ccgcgcagcg caagctctcg ctgatctgcc acggcatcgg ttacgccgag
     1141 gggcggcgtt tccgcgccca ctccgaggcg ctcgagaccc tgcgcgagct cgggctgcgg
     1201 acgaacgcga acaaccgccg cgtcgacgac ctcgaccggg tctacgagta ctgcacgcag
     1261 tggcaggagc accgccacga cgtgccctac gagatcgacg gcgtcgtcgt gaaggtcgac
     1321 tcgatcgacc agcaggagga gctcggcttc accgcgaagg ctccgcgctg ggccatcgcc
     1381 tacaagttcc cgccggagga gcgcacgacg ctgctgcgca agatcagcgt ccacgtcgga
     1441 cgtacgggcg tcgcgacccc gtgggcgcag ctcgagcccg tgttcgtcgg cggcgtgacg
     1501 atctcgacgg cgacgctcca caacgaggac gaggtcgcgc gcaaggacgt gcgagagggc
     1561 gacaccgtga tcgtccggcg agcgggcgac gtgatccccg aggtcgtcgg cccggtgccg
     1621 tcgctccgcc ggaagggcgc gaagccgtgg gtgatgccga agaagtgccc gtcgtgcggc
     1681 gagccgctcg tgcggcagga gggcgaggcc gcgacccgct gcgtgaacct cgaatgtccc
     1741 tcgcaattga tcgagacgct gttccacttc tcgtcgcgcg gcgcgatgga catcgaaggc
     1801 ctcggctaca agacgatcat ctggctgatc gagcgcggat tgctcaagga cgtcgcggac
     1861 gtgtaccagc tgaagccggc ggacttcgag ggccacgagg gctggggtga gatctcgatc
     1921 cgcaacctga cggaggcgat cgaggcgtcg aagaagcggc cgctcggcaa cgtgctgatc
     1981 gcgctcggga tcccgcacgt cgggggccgc gccttcgaca tcgcagcgca ggccgggtcg
     2041 ctcgaccggc tcaaggagat gaccgaagag gacttcctcg cgatggaggg catcggcccg
     2101 gtcatcgcgc gctcgatcac gcagttcttc gcccaggatc gcaacctcga cgtgatccgg
     2161 cgccttcagg aggccggcgt cgacccggtc gaggtcgcac gcaagaagga gggccctctc
     2221 gtcggcaaga cgttcgtgat cacggggacg ctcgagggct tcagccggca aggtgcgcag
     2281 gacgcggtcg aggacctggg cggcaaggtc acgagcagcg tgtcgaagaa gaccgactac
     2341 gtggtggtgg gggccaaccc gggggcctcg aagtacgaca aggcgaagga gctggggatc
     2401 gagctgctgg acgaagcggc gttcacggag ctgctggggc gctaatgctc ggctgggcga
     2461 tcgccttgtc gctgactgct ctggcggtcg tggcgttcgt cctcgcgcac cggaagggga
     2521 cccggcggtt cgaggcggag ctctcgcgga tcgtccgcgg cagcgcggca gagttccgcg
     2581 ccacgggcat gctcccggag cgaccgcgcc ccccgcgccc ccgggtcgtc gtgagagacg
     2641 acgcggtgac cgtgaaggtg cgcgggcgta gggcgcagcg gctggaatgg agccggctcg
     2701 ccgaggtcgc cgtcaggacg acgccgaacg gcccttggca ggaggacgtc ttctttcttc
     2761 tcggcgacga cgcgggctcc gggatcgtgg tcccgcactt ggaggccgcc gatctcctcc
     2821 cgtacctcca gcgcctcccc ggcttcgaca acggggcttt gatcgaggcg atgggatccg
     2881 ccgaagacgc aaacttcgtc tgctggcgcc gcggctgagg ccggccgcag gggaagcccg
     2941 gcccccgtag cctcgcggtg tggggaagaa gagcgcggtc gcccgcgccg tcacagcagt
     3001 gatcctcttc ggcgcgctcc tgcccggtgc cggagccccc gcccgcgccc gcgcccaggg
     3061 ctacgagaac aagtgcggcg gaagcgacgt gacgatcgcc ggtacctccg gcgacgaccg
     3121 gctccgcggg acgcccggca gggacgtgat cgcggccttc ggcgggaacg acgtcgtctt
     3181 cggcctcggc ggcaacgatt cgatctgcga cggcgagggc gacgatcgtg tccacgccgg
     3241 agccgggagg gactcgatcg agaccgtggc aggcatggac acggtgttcg gggagagagg
     3301 cggcgacttc ctgtacgcct tcgcgatcga cgccggcgac tcgaccgact cgttctacgg
     3361 cgggccgggt gacgacatcc tcggggacga ctacggcgac gacctcaccg acggcggtga
     3421 gggcgtcgac acgtaccggg ccctcggcca cgcggcgacg gtgatcgacc tcacgcaagg
     3481 ctacgcggcc gtggcgtccc gaggcgaggt gaacacgctc gtcgcgatcg agaacgcggt
     3541 cgggaccttc tactccgacc agctgatcgg cgacgacggg cccaacgtcc tcgccggcgg
     3601 gatggggccc gacaacgtcc ggggcgaggg cggaagcgac ctgttgctct cagagctgga
     3661 cggcgatcag ctcgacggcg gatccgaccc gacgcgcgac ggcgtgatgg ccgcggtgca
     3721 agggccgatc gcggccgacc tcgtcgccgg gacgctcgcg aggcgaggcg tgaccagccc
     3781 gcaggatcga ctcgttggga tcgaagacgt ggtcggtacg gcggaggacg acctgctcgt
     3841 cggcaacggc gaggtgaacc ggctcttcgg cggccccggg atcgattcgc tggacggtgg
     3901 cgccggggcc gacgtcctct tcggcgacat gccgcccgtc tggtggatca acccgcacca
     3961 ctggagcatc ccgtactacc cgaccacggc cggcaacgac acgctggaag gcggggaggg
     4021 gcacgaccgc ctcaacggcg gggcccggcg cgatctctgc accgggggag aggtcgtgaa
     4081 gcggtgcgag gactcgggcg acgcgggggc ccggccggac gtctcgtact cgctgcgctg
     4141 acccatgaaa aggaaccggc gcagggaatc cctgcgccgg ttccgttctc gttcggctct
     4201 gcaccgcccg cttctcgccg gccgcccttg cggcagttgc ggcgcccgga ggaccaaacc
     4261 cgggacgggt ggcgtcggtg aaccggtgtg gattccgcca gtggcctaag ccgaggtggc
     4321 cagaacccct actgctatga ggcttggacc actaacagcc ggtcacctcg tgaggtccgt
     4381 cgaaactagg taacaagtct ggaccacctc ctttctcacg tacggaaatg aaaacacaac
     4441 gacgcgccta cggacgcgaa tttgcgtgaa atcacgaggc gctggctata ctcgccgggc
     4501 acttgactgc tcggtcaaga accaggccgc ggagccaccc ttaggctcga cgcgcacgcg
     4561 aaggaggagc ctcacaatgc cgtcactcga tgggaaggtc gccgtcgtca cgggggccgg
     4621 gcgcggcatc ggccgcgagc acgccctcgc cctcgcgggg gccggcgcga aggtcgtcgt
     4681 caacgacctc ggcggctcgg ccgcaggcga aggccaggac cagacgccgg cccagcaggt
     4741 cgcagacgag atcaaggcga acggcggcga ggcgacggcg aactacgaca acgtggcgga
     4801 cttcgcgggg gccgagcgga tggtcaagca ggccgtcgcc gacttcgggc gcctcgacat
     4861 cctcgtcaac aacgcgggca tcctgcgcga ccgcatgctc gtgagcatgt cggaggccga
     4921 gtgggacgcg gtcatcgagg tccacctcaa gggccacttc gccccgacgc accacgccgc
     4981 ggcgtactgg agggcgcagt cgaaggaggg caaccagatc aacggccgcg tgatcaacac
     5041 gtcgagcccg tcgggcgtct tcggcaacgt cggccagacg aactacggcg cggcgaaggc
     5101 cgggatcgcg gcgttcacgg tgatcgcggc gcaggagctc ggccgctacg gcgtcaccgt
     5161 gaactgcctc gccccgaacg cccgcacccg catgaccgag gacatcttcg gcatggacaa
     5221 gccggccgac gggttcgatc cgctcgaccc gtcgaacatc tcaccggtcg tcgtcgcgct
     5281 gtgcaccgac gaggctcagg gcatcacccg ccagtgcttc ttcatctacg gcgggacgat
     5341 caacgtgctc aagccgtggg acctgggcac ggcgctccac aaggaggacg gctggacgcc
     5401 cgacgagctc gcaagcgcgc tcacggagaa gttccccagc ggcatcgcgc cggagggcat
     5461 gatgtcgatg ctctcgaagg ccatcggagg aggacagccc cttgcctgaa gacgtgatcg
     5521 agttcgagct gcccgtcgag cgcggcaagg tacgcgagtt cgcgctgtcc gtcggcgagg
     5581 acaacccgat ccacctcgac cccgaggccg cgcggctgca cggcttcccg gacatcgttg
     5641 ccccgccgac gttcaccgtc acgcagatct ggcaggtccc gcgcgaagag cgggaggagc
     5701 gtctcggcgc gaacctcgac tacggccgcg tcctgcacgg cgagcaggag ttcacgtaca
     5761 agcggctccc gttcgccggc gaggtcctga agggccggat gcgaatctcg aaggacttca
     5821 cgaaggaggg acggcgtggt gggtcgatgc gcttcgtgac gtacgagtcg gtcttcaccg
     5881 acgccgagga cgaagaggtc ctcaccgcgt actacacgct gatcgagacg gcgaaggacc
     5941 cgggctcgtg agcgcgccgc agacgatcac gatggaaggc gtctcgccgg gcatggagtt
     6001 cggcgagatg gtgttcggcc ccctcgagcg cgtcgacatc gtcaagtacg ccggcgcgtc
     6061 cggcgacttc aacccgatac acaccgacga gggctacgcg caggcgaccg gggcaccgac
     6121 tgtgttcgcg atggggatgc tgcccgccgg gtacctcgcg cacgcggccg cggactggtt
     6181 cggcgggtcg cagaacctcc ggcgcttcaa ggtccgcttc acgaccagag tgtggccggg
     6241 cgacgagatc gcttgtaccg gccgcgtcgt gtcgatcgag gacggcctcg tgaaggtgac
     6301 gatggaggcg cgcagacggg ggcccgggcc ggacgggctc gacctgccag aagaggagac
     6361 ggcgctgatc ggcgaagccg acgtcgagct gccgcactga cgatccatag gaggacggac
     6421 ccgtgggact gaacaccgag ctcgtcggca aggagtaccc ggcctcgacc ttcgaggtga
     6481 ccgccgacca catcgagcgc tacgcacgcg cgacgaacga cgagaacgag cgctacctcg
     6541 gcggtgacga cgtcgtggcg ccgccggtgt tcccggtcgt acccgcgttc aacacgttca
     6601 tgacggcggc gatggatccc gagctgggcg cggacctgct gaggctcgtg cacgccgcgg
     6661 aggagcacgt cttctacaag ccgatccgcg cgggcgacgt gctgacggtc gcgggagcga
     6721 tcgagtccgt cgagcagaag gagaccggcg agacgttcac ggtcaaaggc accgagacga
     6781 atcaggacgg cgaggtcgtc gccgaggtgc gcgggacgat gttcatccgg ggctcgggct
     6841 ccggcagccg atcggcgacg cctcccgcgg agaacgcgcg cgacgtcgtc tacgaggaga
     6901 ccacgaaggt cgacgacgac cagacgttcc gctatgccga ggcgtccggc gaccacaacc
     6961 ccatccacgt cgacgaggcg accgcgaaga tggcggggct tccggggatc atcctgcacg
     7021 ggatgtgcac gatggccatg gcgtcgaagg gcgcggtgaa cggcctcgcc gggggcgacc
     7081 cgacgcggct cgctcgcgtc ggcgtgcgtc tgtcgaagcc ggtgctgccg ggccaggagc
     7141 tgactacgcg cttctggaag gagtcatctg cttcccaccc ccccagcggg atcgcgacct
     7201 acggcttcga gacctacaac ccggacggcc tggcggtgat caagaacggg atcgtggagg
     7261 tccgggccta gcggaagcag tcggccagct cgaacatccc ggtcctctgg accacgaggc
     7321 ccgcgcctat caccagcacg acgaccgcgg tcgcgatctg tagcggcgcc ggcgccttcg
     7381 gctcggacag acggccgcgg tcgaagccga aggccagggc cgccccggac acgaggcccc
     7441 cgatgtgcgc gatgttgtcg atccgcgcga tcgtgaaccc gagcgcgagg ttgatcagca
     7501 cgaacagcca cagcgactgc aggtgcgggt cgacgaccct cgagctccgc cggttgtaca
     7561 ggtagaccat caacaccccg acgatgccga agaccgcgcc cgacgcgccc aatccgttcg
     7621 agcattgctg gagcccgtac gacgccgcgc tggcgaagaa cccggtcgcg aggtagagga
     7681 acgcgtagcg cgcactgccg aacagctgct cgacctgcgc gccgtagatc gacaggatgt
     7741 acatgttgaa gacgatgtgc atgatcgcgt acccgccctc gctcagcggc aggtgcagga
     7801 agaccgacgt gaggagccgg tagacctcgc cgtcggcgat ggccggcgcg agcgacgtga
     7861 agcggttcac gaactcgttg ccgccgaaca gctcgaccac gtacacggcg accgtcaggg
     7921 cgatgatcgc cctcgtgacc ggggcgttga cggccatcgc cgtccggacc ttcggcgcgc
     7981 tcttgcgggc ctccgctacg cactcggggc agtgctggcc gaccgacgcc tggatcgcgc
     8041 actgcccgca gatgtagcgg tcgcagcggg tgcattggag cttcgtcggc cggtcggggt
     8101 ggccgtagca gtaggtctcg acgttctctt cggcggtctg ggtcccctcc ggcggattca
     8161 cgcgggcaat gctcgcacac gggcttcctt atcctgcaag agatgcgggt cgcgacgttc
     8221 aacgtccatc actgcgaggg ccgcgacggc gcggtcgacg tcgcccgggt ggccgacgtg
     8281 atccgtgcga cgggggccga ggtcgtcgcg ctccaggagc tcgacatggg cctcgaacgg
     8341 acgggcttca cgaaccagcc gggcgagctc gcggccgcca ccggcctcac cgtcgagttc
     8401 cactcgctgc tccaacgcgg cgacggacgc tacgggatcg cgctcgcgtc aaaggagcag
     8461 cgtctcgaca gcgagctgat ccacctgccg caacaggcac gggaggaacc gcgcggcgcc
     8521 gtcgtcgccc gccggggcgg catcacgttc gtcggcacgc acctgtcgcg ctcccgcagg
     8581 gcccgcgcgg cccagatcga ggcgctcgcc gtcctcgccg gccgggtcga gcctcccgtc
     8641 gtgctgatgg gcgacctgaa ccaggcggcg tccgggctcg gccccctgct cgccgccggg
     8701 ttcagggggc ctccccgccg gtggtggacc cgccccccga gatcgatcga ccacatcctg
     8761 gcggggccgg gcctgcgagt cgcgtcgcag cagatcctcc ggacgaaggc ctccgaccac
     8821 ccggctctgg cggccgagat cgtgcgagag gagtgactgg agctccctgt gttactgatg
     8881 tggcttctgt taatgtctga ggtccaaaga gactgcagat tgccgcgggg gccggggagg
     8941 ctcatgtgag catttcgatc atgacgagag cggccgctct ggtgctggcg gcgggagcat
     9001 tcgcgggctg cgcccgcgcc gaggctctcg atcccgccgc gaaggagagg ctcgacgagg
     9061 cgaacgccac ggcgtccgag gcggtcgagg ccgtcgacgc cctcgaggcc cgcatcgacg
     9121 agctcgaagc ccagctcgac gacgccgaac gcctcggcga ccgcgtcgac caggtctcgg
     9181 aacgcctgtg ggagtcgctg gccaagctcc gcaagtccct cgacgacgtc cggggtggag
     9241 cttcggatgc ggccgcgacc gcggacgcgg cagcgggcca ggcgtcgcaa gccgctcggg
     9301 acctggcggt cctcggcaac cgctacgact accacctgcg ccgctaccac ggaggcggat
     9361 agatgcgcct gatgcgcgac ctcgtcggcg agacgctctc ggaccggtac cggctcgtag
     9421 cgaggatcgc cggcgggggc atgggcgagg tctaccgcgg ccaggacgta ttgctcgagc
     9481 gtccggtcgc cgtgaagatc ctgcagccgt cgcttgccgc cgacccggat ctcgtcgaac
     9541 gcttcaagga cgaggcccgt gccgcggccc gcctgacgca cccgaacatc gtgtcggtcc
     9601 acgactgggg cgcggccgac gagcagacct actacatggt catggagttc gtggccggga
     9661 ccgacctgcg cgacctcctc gtgagcagag gttcgctcgc gccggcgcag gcggcggaga
     9721 tcatggcgtg cgtgtgcgac gcgctcgcgg ccgcgcacgc ggcgggcgtc gtccaccgcg
     9781 acgtcaagcc cgagaacgtg ctgatcgcgc gcaacggccg cgtgaaggtc gcggacttcg
     9841 ggatagcggc ggtcgccgac gcggaccgga cgatgcccgg cgggggcatc cccgggacgc
     9901 tgcgttatct ctcgccggag caggcgtcgg gccgagcggc ctcgccggcc tcggacgtgt
     9961 gggctgcggg ggccgtgctg tctgagctgc tgacggggcg cccgccacag caggggagcg
    10021 gtgccgacct cctgcgccgc cgcgcgatcg agccgccggt cgcgccgtcg acgttcgacc
    10081 agacgctgcc gcaggcactc gacgagatcg tcatgaaggc ttgcgcgctc gacccccccg
    10141 agcgcttcga cgacgccgcg gagatggccg cggcgctgcg gcgggcgtcg gtgcggtcgc
    10201 tcccggacgc gccgccggtc gactcgctgc tcgacgacgt gacgggagtc atccggctcc
    10261 ccgacgacga caagcgcgag atcacacgtt cgaagcggcg tcgccgcggc cgccggatca
    10321 aggtcggagc ggctctcgtg gtgctcgcgc tgctcgcgtt cggtgccgcg aaggcggtcg
    10381 cgttgctggc ggcccccgcg cgcgtcgacg tcccgacgct gacgggcctg ccgcgcgcag
    10441 acgcgcgcgc ggaagcggaa gaggcggggc tcgagctcga gatcgtgggc cgcgacaccg
    10501 acctcgacgt cccgaagggt cacgtgctgt cccaggaccc cgcagacggg accttgctcg
    10561 aaggcaaggc gatcgcggtc gtcatctcgg gcgggctgcc gaagcacgaa gtgcccgacc
    10621 tgatcggcat gaaggtcgac gcggccacga cgcgtgcgaa cgtgtacggc ttcgagatcg
    10681 tcgtcaccgg cgaggagttc gcgctcacgg accccggcga gatcatcgag cagacgccgg
    10741 cggacgggaa gctcgaacga ggcgggaagc tcgaggtcgt cgtcagcaag gggccccagt
    10801 cgatcggcgt cccgaacgta gagggccttc ctgcgacgaa ggcccagaag atgctcgagc
    10861 gcgagggctt cgaggtcgtc gccgtgcccg tctactcgaa caccgtcgac ctcggcatcg
    10921 tggtctacac gacgccgccc ggaggaagca cggcccccga ggggagccgt gtcgacatgg
    10981 cggtgtccca gggaccgaag tacgacgagg tgacgatgcc cgacgtgcgc gggatgacga
    11041 tcgacgcggc cacctcggag ctcgaaggcc tgggattgcg cgtcaacgtc gtccagtcgt
    11101 gcggaggcgg gggcacgatc gtgtccgaga cgcagccgtt gggaggaacg aagatccgcg
    11161 agaaccagct cgtggacgtc ttcacctgct agcccggcgg gaggatcccc cacccgcgta
    11221 gcgtctcgtg gaagcgcggc gtgcacggca tcgacgggac ctcctccagc gggacccagc
    11281 gcgcgtccga gacgtcgtcc gcggggaccg gagcgtcgtc gcc
//
LOCUS       DASQDO010000003         1965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_796223, whole genome shotgun sequence.
ACCESSION   DASQDO010000003 DASQDO010000000
VERSION     DASQDO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796223"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VEV43_00100"
     CDS             <1..618
                     /locus_tag="VEV43_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYP21954.1"
                     /translation="DWPEEGVTAIGRVTFPWLKRVWRLCEENRDVVSVGELHMGPAEN
                     ALRKHIHKTIKVVTDDFSTKSFNTAIARLMELVNNAGRYKTVGGGHPGVMRELLETLL
                     KLLAPFAPYVTEEQWHRLGHDTSIHKEPWPVYDAALAADDEITMVVQVNGKVRDTIKV
                     SPDVDEETMKELALASEKVAGHLGGNPPAKIIVKPPKLVSLVAAR"
     gene            complement(615..1835)
                     /locus_tag="VEV43_00105"
     CDS             complement(615..1835)
                     /locus_tag="VEV43_00105"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP21955.1"
                     /translation="MEIRLRGIEPGDARKWLGVTEVSFGAGLPDDVFEDLRHVVETDR
                     SIAAFDGDTMVGTAGAYSLKLTVPDGEVDCAGVTMVGVLPTHRRRGVMTAMMTQQLRD
                     VAERGDPLAALWASEDAIYGRFGYGPATVQGLIDVPRHRATFLDDGPIEGTMRLLDRD
                     EAVASFPAVYDKVRPTRPGMFHRSEKWWRHRVARNPTSDEEGPFFRALLEQDGEPVAY
                     AVYRVQQKWEQGVSRGSVILREVMAATPRGERDVWKFLFGIDLVESVGSPSFFLPSDH
                     PLQLMLAEPRYLNFLLSNGLWLRVVDVGAALEARGYSADGTITLEVHDPLLEGNSGTW
                     RLEASGGRAKATKTGGEGDVRLGIAELGALYLGQFTFTDMLAAGRIEASPEAVERADA
                     MWRTARAPWCPEIF"
BASE COUNT          322 a          686 c          672 g          285 t
ORIGIN      
        1 gactggccgg aggagggcgt gacggccatc ggccgcgtca ccttcccgtg gctgaagcgg
       61 gtctggcgtc tctgcgagga gaaccgcgac gtggtctcgg tcggcgagct ccacatgggg
      121 ccggcggaga acgccctgcg caagcacatc cacaagacga tcaaggtcgt caccgacgac
      181 ttctcgacga agtcgttcaa caccgcgatc gcgcggctga tggagctcgt caacaacgcc
      241 ggccgctaca agaccgtcgg tgggggacat cccggcgtga tgcgcgagct gctcgagacc
      301 ctgctgaagc tgctggcgcc gttcgccccg tacgtcacgg aggagcagtg gcaccggctg
      361 ggccacgaca cctcgatcca caaggagccg tggccggtct acgacgccgc cctggcggcc
      421 gacgacgaga tcacgatggt cgtccaggtg aacggcaagg tgcgggacac gatcaaggtc
      481 tcaccggacg tcgacgagga gacgatgaag gagctcgcgc tcgcatccga gaaagtcgcg
      541 gggcacctcg gtgggaaccc gccggcgaag atcatcgtga agccgccgaa gctggtgtcg
      601 ttagtagcgg ctcgctaaaa aatctccggg caccacgggg ccctggccgt ccgccacata
      661 gcgtcggctc gttccacggc ctccggtgaa gcctcgatcc gtcctgccgc gagcatgtcc
      721 gtgaacgtga actggccgag gtagagcgcg ccgagctcgg cgatgccgag acggacgtcg
      781 ccctcgccgc cggtcttcgt cgctttggcc cggcccccgc ttgcttcgag cctccacgtc
      841 cccgagttcc cctccagcag cgggtcgtgg acctccaggg tgatcgtccc gtcggctgag
      901 tagccgcgcg cctccagcgc cgctccgacg tcgaccaccc gcagccacag cccgttcgac
      961 agcaggaagt tcaggtagcg gggctcggcc agcatcagct ggagcgggtg gtcggacggc
     1021 aggaagaacg acggcgatcc cacggactcg acgaggtcga tgccgaagag gaacttccat
     1081 acgtcgcgct cgccgcgcgg cgtcgcggcc ataacctcgc ggagaatgac gcttccgcgt
     1141 gagaccccct gctcccactt ctgctgcacg cggtagaccg cgtacgcgac gggctcgccg
     1201 tcctgctcga gcaacgcgcg gaagaacggc ccctcttcgt cactcgtcgg gttgcgggcg
     1261 acgcggtgcc gccaccactt ctccgagcgg tggaacatgc ccggcctcgt cggacggacc
     1321 ttgtcgtaga cggccgggaa ggacgcgacc gcctcgtcgc ggtcgaggag acgcatcgtc
     1381 ccttcgatcg gcccgtcgtc gaggaaagtc gcacggtggc gtggtacgtc gatcagaccc
     1441 tggacggtcg cggggccgta gccgaagcgt ccgtagatcg cgtcctcgct cgcccacaac
     1501 gccgcgaggg gatcgcctcg ttcggcgacg tcgcgcagct gctgcgtcat catcgccgtc
     1561 atgacgccgc ggcggcggtg cgtcggcagg acgccgacca tcgtgacgcc ggcgcagtcg
     1621 acctcgccgt cgggcacggt gagcttcagc gaatacgcgc cggccgtgcc gaccatcgtg
     1681 tcgccgtcga acgccgcgat cgagcgatcg gtctcgacga cgtggcgcaa gtcctcgaac
     1741 acgtcgtcgg ggagcccggc gccgaacgag acctcggtga ccccgagcca cttacgcgcg
     1801 tcgcccggct cgatcccgcg cagtcgtatc tccatggcca ccgatgatgg cacgcacccg
     1861 tgtggccccg gccacagcgc gcgagcggcc ctgccgcgac cgtggggcgc atgaacgacg
     1921 tcgtcgtgcc cgcggggccg aaggcgcggc tcgaggaggt catcg
//
LOCUS       DASQDO010000004         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_306760, whole genome shotgun sequence.
ACCESSION   DASQDO010000004 DASQDO010000000
VERSION     DASQDO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306760"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /gene="mca"
                     /locus_tag="VEV43_00110"
     CDS             <1..873
                     /gene="mca"
                     /locus_tag="VEV43_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HYP21956.1"
                     /translation="PSPVTKTQKCLLAIHAHPDDESSKGSGTMAKYANEGVHVALVCA
                     TDGAEGDILNPRMARPGIKERMAEIRKAELETACDLLGVHRIYHLGYRDSGMPESEAN
                     KHPQAFWNADPDEALRKLVEIIRAERPEVVLCYDESRGYEHPDHIRVYEWGTQAFDLA
                     SDGSKWPDAGAPWQPAKLYYFATFTKKRFQLLNDAAVAEGHEPPYAGWLENWDSFGFD
                     EPEITTQIDVADYVELRSKALLAHATQIDPDSFWFAVPDEMQRKVYPWEDYTLVKSTV
                     KTDLPESDLFAGID"
     gene            complement(884..1738)
                     /locus_tag="VEV43_00115"
     CDS             complement(884..1738)
                     /locus_tag="VEV43_00115"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HYP21957.1"
                     /translation="MADPADQAPLPRLTAPRADVERELRSQVSKGHGYQGVPVSSPDD
                     LRSLLGKVRGWNDHNVELLKSRFTTFRVVNHYESVGPPFEVAFDIDKSLAFLRDYLRQ
                     KTAYLSTLKKRLDLYDEPIEGGPAPARTELRKETIFVVHGHDRAAELEVLRFLERATD
                     LRPVVLHDEPNRGRTIIEKFEEVAANATYAVVILTPDDEGRAQNAPDLKPRARQNVVF
                     ELGFFFGALDRNRVAALYTPEIERPSDIDGVAYIEFDRRGAWKNDLYRELRDADITVH
                     PEALVPLG"
     gene            complement(1778..>2766)
                     /locus_tag="VEV43_00120"
     CDS             complement(1778..>2766)
                     /locus_tag="VEV43_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP21958.1"
                     /translation="TFLPSHRSIKKREGKYANVLIIAATNRADTLDPALLRPGRFDRI
                     LHFDLPSRAGRRELVDYFLERRKHDPELDREDLREEFASMTLGYSPAMLEHVLDEALV
                     WALRDDRRELNWRDVQRARLTQEIGIAQPVAYTERERDLIATHEAGHAVAAYLCAKDR
                     KLEVLSIIKRRSALGLLAHSDAEERFTKTKSELEGTLKIALGGMAAEQLFFGESGTGP
                     SSDLQAATTLAAQMVGSFGMGSSLVSYEAVDNGPHGSPNIVAKILSNDDTKREVDEIL
                     RKHKDQVAYLLEANKDLVEALRDALLEREELLGDEIMSVLDDAQKARAEMLG"
BASE COUNT          466 a          909 c          921 g          470 t
ORIGIN      
        1 cctagccccg tgaccaagac acagaaatgt ctcctcgcga tccacgccca tcccgacgac
       61 gagtcctcga agggctcggg gacgatggcg aagtatgcga acgagggcgt gcacgtcgcg
      121 ctcgtgtgcg cgaccgacgg cgccgagggc gacatcctca acccccgcat ggcccggccg
      181 gggatcaagg agcgcatggc cgagatccgc aaggccgagc tcgagaccgc gtgcgacctc
      241 ctcggcgtgc accgcatcta ccacctcggc taccgcgaca gcgggatgcc cgagtccgag
      301 gcgaacaagc acccgcaggc gttctggaac gccgatccgg acgaggctct gcgcaagctg
      361 gtcgagatca tccgggccga acgccccgag gtcgtcctct gctacgacga gtcgcggggc
      421 tacgagcatc ccgaccacat ccgcgtatac gagtggggca cgcaggcgtt cgacctggcg
      481 tccgacggat cgaagtggcc ggacgccggc gcgccgtggc agccggcgaa gctctactac
      541 ttcgcgacgt tcacgaagaa gcggttccag ctgctgaacg acgccgcggt ggccgagggc
      601 cacgagcctc cgtacgcggg ctggctcgag aactgggact cgttcggctt cgacgagccg
      661 gagataacga cgcagatcga cgtggcggac tacgtcgagc tgaggagcaa ggccctgctc
      721 gcgcacgcga cgcagataga cccggacagc ttctggttcg cggtgcccga cgagatgcag
      781 cgcaaggtct acccgtggga ggactacacg ctggtgaagt cgaccgtgaa gacggacctg
      841 ccggagtcgg acctcttcgc gggcatcgac tagccgaggt ctcttagccg agcgggacca
      901 aggcttccgg gtggaccgtg atgtctgcgt cgcggagctc acggtacagg tcgttcttcc
      961 acgcaccgcg ccggtcgaac tcgatgtatg caaccccgtc gatgtcggag ggacgttcga
     1021 tctcgggcgt gtagagagcg gccacgcggt tcctgtctaa ggctccgaag aagaagccca
     1081 gctcgaagac gacattctga cgcgcacgcg gtttcaggtc cggtgcgttc tgcgcccgcc
     1141 cttcgtcgtc tggcgtcagg atgacgacag cgtacgttgc gttcgcagct acctcctcga
     1201 acttctcgat gatggtgcgc ccgcggttcg gctcgtcgtg caaaacgacc ggacggaggt
     1261 cggtcgcgcg ctccagaaac ctcagaacct cgagctccgc agcacgatcg tggccgtgca
     1321 ctacgaagat cgtctctttc cggagttccg tgcgagcggg agcggggccg ccctcgatgg
     1381 gttcgtcgta aaggtcgagt cgcttcttca gcgtcgagag gtaggccgtt ttctgccgga
     1441 ggtaatcgcg gaggaaagcg aggctcttgt cgatgtcgaa ggcgacttcg aaggggggtc
     1501 ctaccgattc gtagtggttc acaacccgaa acgtcgtgaa gcggctcttg agaagctcga
     1561 cgttgtggtc gttccacccg cgaactttcc ccaggagcga tcgaagatcg tcgggactcg
     1621 acaccgggac gccctgatag ccatgtccct tcgagacctg cgaccgtagc tcgcgctcga
     1681 cgtcagcccg gggtgccgtg aggcgcggca gaggggcttg gtcagctgga tctgccatcc
     1741 acagaactta cacaggcgtg gggcagccag ttaactgcta gccgagcatc tccgcgcggg
     1801 ccttctgggc gtcgtccagg accgacatga tctcgtcacc cagcagctct tcacgctcca
     1861 gcagcgcgtc gcgcagggcc tcgaccaggt ccttgttcgc ctccagcagg tatgcaacct
     1921 gatccttgtg cttgcgcagg atctcgtcta cctcacgctt ggtgtcgtcg ttgctcagga
     1981 tcttggccac gatgttcggg ctcccgtgcg gcccgttgtc gaccgcctcg tatgacacca
     2041 gcgacgagcc catcccgaac gagcccacca tctgcgcggc cagcgtcgtc gcggcctgca
     2101 ggtcggacga cggcccggtc cccgactcgc cgaagaacag ctgctcggcc gccatgcccc
     2161 cgagcgcgat cttcagagtc ccctcgagct cggacttcgt cttcgtgaag cgctcctcgg
     2221 catcggagtg cgcgagcagg ccgagggccg aacgccgctt gatgatcgag agcacctcga
     2281 gcttgcgatc cttggcgcag aggtacgcgg cgacggcgtg gccggcctcg tgcgtcgcga
     2341 tcaggtcacg ctcgcgctcc gtgtaggcga cgggctgcgc gatcccgatc tcctgcgtga
     2401 gccgcgccct ctggacgtcg cgccagttca gctcgcgccg gtcgtcacgc agcgcccaca
     2461 ccagggcctc gtcgagcacg tgctcgagca tcgcggggct gtagccgagc gtcatcgacg
     2521 cgaactcctc gcgcaggtcc tcgcggtcga gctcggggtc gtgcttgcgg cgctcgagga
     2581 agtagtcgac gagctcgcgg cgtcccgccc gggacgggag gtcgaagtgc aggatccggt
     2641 cgaaccggcc cggccggagc agcgcggggt cgagcgtgtc ggctcggttc gtcgccgcga
     2701 tgatcagcac gttcgcgtac ttgccctcgc gcttcttgat cgaacggtgc gagggcagga
     2761 acgtgt
//
LOCUS       DASQDO010000005         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_551613, whole genome shotgun sequence.
ACCESSION   DASQDO010000005 DASQDO010000000
VERSION     DASQDO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551613"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..997)
                     /locus_tag="VEV43_00125"
     CDS             complement(167..997)
                     /locus_tag="VEV43_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21959.1"
                     /translation="MEELVYADRSDRGNLRFTGPQRAWFLHQIMTQAFEDMAPGDARD
                     AAMITAHGRMLGYLEVAATDDALLAHFEPSLLATLPHEIRRYVFATRVEIEDVSADMG
                     LVLLAGEGWESAAARVEGAIVHPTSSLGIPAAYLWVTDTAAALAALKENAREATEDEL
                     EAVRVAHGVPRWGYEMDLKTIPQEAGIEARAVHFDKGCYLGQEAMAKIHFRGKVNRRV
                     VLIQSENRLERGAEVVLDGATVGKVTSVAGSRGLALVKHTVGPDAQVSVGESPARVVS
                     "
     gene            1022..>1911
                     /locus_tag="VEV43_00130"
     CDS             1022..>1911
                     /locus_tag="VEV43_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP21960.1"
                     /translation="MNFDLPDELVAFRESAREFARREIAPRVEEAERTERTPRDLFRA
                     AGRAGFLGIRYPESVGGAAASCLAETILREEIGYVCSGLGAALSVSSHLGTYPIHAFG
                     TEEQQERYLRGALTGEKVSAFALTEPDAGSDVRAIRTRAVRSGDGWVINGRKTFITNA
                     PIADFMIVVAYTDPDAGIEGMALFVVDLPATGVVINRLSKMGNLSSEIAEVVLDDVVV
                     PAEACLGGERGAFKKVMQTLNVGRVLVSGGALGVARAALDGAVAYANDRHAFGRPIGS
                     YQGVSFPLARSHATIEAARLA"
BASE COUNT          263 a          645 c          691 g          312 t
ORIGIN      
        1 cgcgccgctc gcgccggcga ggatcgtctc aggctcgaag cagccgtcga cgatgtacgc
       61 gtcgtgcgtc tctggacact cgagcacgta gacgttgttg cccatcgagc ccacgccgat
      121 cttgcggacg acgaggtcgt cgccttcgta gtggtgggcc acctcgtcag ctcaccaccc
      181 gggccgggga ctcgccgacc gacacctgcg cgtcggggcc gacggtgtgc ttgacgagcg
      241 cgagcccgcg tgagcccgcg acgctcgtga ccttcccgac cgtggcgccg tcgaggacga
      301 cctcggcccc ccgctccagc cggttctcgg actggatcag cactacgcgg cggttgacct
      361 tgcccctgaa gtggatcttc gccatcgcct cctggccgag gtagcagccc ttgtcgaagt
      421 ggacggcccg cgcctcgata ccggcttcct gcgggatcgt cttcaagtcc atctcgtagc
      481 cccagcgcgg gacgccgtgc gcgacccgga cggcttcgag ctcgtcctcg gtcgcttccc
      541 gtgcgttctc cttcagggcc gcgagcgcgg ccgcggtgtc cgtcacccag aggtatgcgg
      601 cggggatgcc gagcgacgaa gtcggatgga cgatcgcgcc ttcgacgcgg gccgccgcgc
      661 tctcccatcc ttcgcccgcg agcagcacga gccccatgtc cgcggagacg tcctcgatct
      721 cgacccgcgt ggcgaacacg tagcgcctta tctcgtgggg gagggtcgcg aggaggctcg
      781 gctcgaagtg cgcgaggagc gcgtcgtccg tcgccgcgac ctcgaggtac ccgagcatgc
      841 ggccgtgcgc ggtgatcatg gcggcgtcgc gggcgtcgcc gggagccatg tcctcgaacg
      901 cctgcgtcat gatctggtgc aggaaccatg cgcgctgagg ccccgtgaac cgcagattgc
      961 cgcggtccga ccggtcggcg tagacgagct cctccatggg cccatcttct atcctgcgcc
     1021 ggtgaacttc gacctgccgg acgagctcgt cgcgttccgc gagtcggcac gcgagttcgc
     1081 ccggcgggag atcgcgccga gggtcgagga agcggagagg accgaacgca cgccgcgcga
     1141 cctgttcagg gccgcgggcc gcgccggatt cctcgggatc cggtatccgg agtccgtcgg
     1201 gggtgcggcc gcgagctgcc tggccgagac gatcctgcgc gaggagatcg gctacgtgtg
     1261 ctccggtctg ggggccgctc tgtcggtgtc gtcgcacctc ggcacgtatc cgatccacgc
     1321 gttcgggacc gaggagcagc aggagcgcta cctgcggggt gcgctgacgg gcgagaaggt
     1381 gagcgcgttc gctctgaccg agcccgacgc cggctccgac gtgcgggcga tccgcacgcg
     1441 cgccgtgcgc agcggggacg gctgggtgat caacgggcgc aagacgttca tcacgaacgc
     1501 gccgatagcc gacttcatga tcgtcgtcgc gtacaccgat cccgatgcgg ggatcgaggg
     1561 catggctttg ttcgtggtcg acctcccggc caccggggtt gtgatcaacc ggctgtcgaa
     1621 gatgggcaac ctctcgtcgg agatagcgga ggtcgtgttg gacgacgtcg tcgttcctgc
     1681 cgaagcgtgc ctcggcggcg agaggggcgc gttcaagaag gtgatgcaga cgcttaacgt
     1741 cgggcgcgtg ctcgtgtcgg ggggcgctct cggcgtcgcg cgcgcggccc tcgacggcgc
     1801 ggtcgcgtac gcgaacgaca ggcacgcgtt cggccggccg atcgggtcgt accagggtgt
     1861 gtcgttcccg ctggcgcgtt cgcacgcgac gatcgaagcg gcgaggctgg c
//
LOCUS       DASQDO010000006         6758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_306814, whole genome shotgun sequence.
ACCESSION   DASQDO010000006 DASQDO010000000
VERSION     DASQDO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306814"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VEV43_00135"
     CDS             <1..225
                     /locus_tag="VEV43_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21961.1"
                     /translation="IGINCCIAGSDQLPCCCSDANDVTTCAGVCRSQAACAGLFPGGP
                     AEDPDSCFYPGGTGTATMARRRVKAKVVNT"
     gene            289..1581
                     /locus_tag="VEV43_00140"
     CDS             289..1581
                     /locus_tag="VEV43_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HYP21962.1"
                     /translation="MELTERIHEADARVAIIGLGYVGLPLALAAADAGFEVLGFDTSA
                     DRVAALRDGTSPVTDVSDDELRAAVERGAFRPTALPEDLQGSDVFVICVPTPLRDELP
                     DMSYIESAGNLIAEQMKPGALVVLESTTYPGTTEEFLRPILEKGGLIAGGDFHLGFSP
                     ERIDPGNPEYGLKNVPKIVGGVDETSTALMEAFYGKLVDRVVTVSSPMTAEMAKLLEN
                     TYRHINIALANEMAIVCHDLGISIWEVIDAASTKPFGFHPFYPGPGWGGHCIPVDPAY
                     LSWRVRQMGGTAHFVELAREINARMPAYVVQRITDALNDEGKSLKASRILLLGVAYKP
                     DVGDVRESPALEILARLRKAGANVSFHDPYVEEVRVAGEVIPVRTLDSAALRESDIVV
                     AITNHSTYDWQDISDRSRLVLDTRNALAGITGGNVVTL"
     gene            complement(1578..2669)
                     /locus_tag="VEV43_00145"
     CDS             complement(1578..2669)
                     /locus_tag="VEV43_00145"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYP21963.1"
                     /translation="MTGDHFIPPVRLDNGDVWDALAPRIEDLVRRGGFVLGPELEEFE
                     AAAAEAFGCRWAVGVSSGTSALWMALRALDLPPGARVALPANTFFATCEAVLVAGMTP
                     VIVDNDEDYLIDLDGLEGADVDAVIPVHLYGLPVDMARVEELARRNGWRVLEDCSQSH
                     GATSGGRVTGSIGDAGAFSAYPTKNLGAWGDAGFATGSDESVGATIRSLRHHGQRDAN
                     VHSEVGSPERLDNLQALVLTEKLRRLPAEIEARRRVAGWYRDALEGLDLALPGDRGDR
                     THVYHQFVIRVPRRDEVREQLRDLGVGSGVHYPAPVHLQPAVAELCAIAQRPERAEAW
                     AGELLSLPFFPSLAEGEVERVAEALRKVL"
     gene            2708..3187
                     /locus_tag="VEV43_00150"
     CDS             2708..3187
                     /locus_tag="VEV43_00150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYP21964.1"
                     /translation="MAGPHDAPFSLINDVDFGDGVRLAGFVNLYGCTIGDETFVGPFV
                     EVQRGVTIGRRCKIQSHTFLCTGVHIGDEVFVGHGVVFTNDRAPRATNAAGEIAGDDD
                     WMLEETHVGRRAAIGSGAVILPGLTIGEGATVGAGAVVTKDVPAGAVVTGVPARTRV"
     gene            complement(3184..4245)
                     /locus_tag="VEV43_00155"
     CDS             complement(3184..4245)
                     /locus_tag="VEV43_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21965.1"
                     /translation="MSRVKCSVRVAGNAQHVYQVVTGFAMLGERREVDASFDVVPATE
                     APVSSFNVLTATIDGRRVAYDMLDGYRNIPRPEMERLLSEVDCYFKRSFSERHNAELQ
                     QGDRIHPFGLSYVVTCRASVFRTMRSRDPLRQKASRAAKAIMGRSAYPRVRVFEAPPQ
                     VRDDPKVVFMTRVYDPSGEPGEDPQALLHGNAGPEREQINEMRAGCVRALREAFGPSF
                     SGGLAPTAYGTARYPDCVSDAAALPRTQYLRAAKKADVGIATMGLHESNQWKLAEYVA
                     ASRAIVSERLRYQVPGFSQPQNYLEFETPEECVERTADLLGSRDRRLAMMRENARHYE
                     AYVRPDSAVARTIEVVRAL"
     gene            complement(4242..6491)
                     /locus_tag="VEV43_00160"
     CDS             complement(4242..6491)
                     /locus_tag="VEV43_00160"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP21966.1"
                     /translation="MSATRVVVMNASLNVGGAERTLADLARRLDRRRFEVTVVCLYEA
                     GPVGEALRSEGVTVVDRVLRHKADVRGFLRLVSLLRRLAPHVLYYNNQPLTQLWGWTA
                     AVLARVRATVTVFHFASRREKAGRGVLLNRMLGRRVDACVALSQAHKAYVSATERVPE
                     RKIVVIPNGIDVGAFSAARDARDEVRSDLGIAPDRTVVSIVAQLRPEKNHSMFLRAAR
                     EVLEWGARVTFLVVGDGVERPRLERLARELGIERDVRFLGTRSDVPAVMAASDVGVLC
                     SFGEAFPLSVLEFMAAGRPVVSTEVGSVDEIVLPGETGFLVPRDDAAALAVKLERLIT
                     DDGERKRLGDAAKRRVEEHFTVERMVERTEALLEDVTGRSGAGATGRQTVIVVGPRLR
                     LWGGVNTHVRTLLSGGLNEAFDLVHLEVGFYEESASRAKRLIDGLRKIAQLRKLVARR
                     PGAVVHLNPSMDTRSLIRDLPMVHVASRRGARVVVQFHGGLMDRPKPMRYGWVRRAVL
                     RTLERASLVVVLSRLQARSISSTLGASERMKIQQVPLFLDLQPFEARRRERPERDLPR
                     SFVFVGRLVQEKGVHELVAAVAALRSDGLDVVADIAGAGPDESEMRRRCDALGVGGAV
                     TWHGYLGTESKLDLLARSDVFVLASSWLEGLPNALLEAMAMGLPVIVSDVGAMAEVVE
                     DGVNGFLVPAGDAGALADKMAYLVRHGSEAAAMGRRNSAIATARFGLDSQVTTWGDIY
                     TRVSRRSAQ"
     gene            complement(6488..>6758)
                     /locus_tag="VEV43_00165"
     CDS             complement(6488..>6758)
                     /locus_tag="VEV43_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP21967.1"
                     /translation="QVVAEAMAAGLPVVASAVGGVPWMIEDGVNGYLFSPRDVDALAE
                     RIVGVLSGDSTDLAAKARAEARRFAPAHVAAETVDLYRALVGVRR"
BASE COUNT         1054 a         2447 c         2259 g          998 t
ORIGIN      
        1 atcggcatca actgctgcat cgccgggagc gaccagctcc cgtgctgctg ctcggacgcg
       61 aacgacgtca cgacctgcgc cggcgtgtgc cgcagccagg ccgcgtgcgc ggggctcttc
      121 cccggcggcc cggccgagga ccctgactcg tgcttctacc cgggcggcac cggcaccgcg
      181 acgatggccc ggagaagggt caaggccaag gtcgtcaaca cgtagcgtct ccacgatccc
      241 ggacgcccct acctcggtgg gggcgttctt cgttggtagc ctgccgccat ggagctgacc
      301 gagaggatcc acgaggccga tgcccgggtc gcgatcatcg gcctcggcta cgtcgggctc
      361 ccgctcgcgc tcgccgccgc cgacgcgggc ttcgaggtgc tgggcttcga cacgtcggcg
      421 gaccgggtcg cggcgctgcg cgacggcacg tcccccgtca ccgacgtatc ggacgacgag
      481 ttgcgggcgg cggtcgaacg cggggcgttc cggcccaccg cgctgcccga ggacctgcag
      541 ggctccgacg tcttcgtgat ctgcgtcccc acgcccctgc gcgacgagct gcccgacatg
      601 tcgtacatcg agtcggccgg gaacctgatc gccgagcaga tgaagccggg cgcgctcgtc
      661 gtcctcgaga gcacgaccta ccccggcacc accgaggagt tcctgcgacc catcctcgag
      721 aaggggggtc tcatcgccgg cggggacttc cacctcgggt tctcgcccga gcggatcgac
      781 cccggcaacc ccgagtacgg cctgaagaac gtgccgaaga tcgtcggcgg cgtcgacgag
      841 accagcaccg cgttgatgga ggcgttctac ggcaagctcg tcgaccgcgt cgtgaccgtg
      901 tcgagcccga tgacggccga gatggcgaag ctcctcgaga acacctaccg gcacatcaac
      961 atcgcgctgg cgaacgagat ggcgatcgtc tgtcacgacc tcggcatcag catctgggag
     1021 gtcatcgacg cggcctcgac gaagcccttc ggcttccatc ccttctaccc tgggccgggc
     1081 tggggcggac actgcatccc ggtcgacccc gcctacctgt cgtggcgcgt ccggcagatg
     1141 gggggcaccg cgcacttcgt cgagctggcg cgcgagatca acgcgcgcat gcccgcctac
     1201 gtcgtccagc ggatcaccga cgcgctcaac gacgagggca agagcctgaa ggcgtctcgc
     1261 atccttctgc ttggggtcgc atacaagccc gacgtcggtg acgtgcgcga gtcgccggcg
     1321 ctcgagatcc tcgcgcggct tcgcaaggcc ggcgcgaacg tctcgttcca cgacccctac
     1381 gtagaagagg tgcgggtggc cggcgaggtg atcccggtgc ggacgctcga ctccgcggcc
     1441 ctgcgcgagt ccgacatcgt cgtcgcgatc acgaaccact cgacgtacga ctggcaggac
     1501 atctccgacc ggtcacggct cgtgctcgac acgcgcaacg cgctcgccgg gatcaccggc
     1561 ggcaacgtcg tgactctcta gagcaccttc cgcagggcct ccgccacgcg ctcgacctcg
     1621 ccctcggcca gcgacgggaa gaacgggagg ctcaacagct ctcccgccca ggcttcggcc
     1681 cgctcgggcc gctgcgcgat ggcgcagagc tccgcgaccg ccggctgcag gtggacgggg
     1741 gccgggtagt ggacgccgct gccgaccccg aggtcgcgca gctgctcgcg cacctcgtcg
     1801 cgccgcggca cccggatcac gaactggtgg tacacgtgcg tccggtcgcc gcggtcgccg
     1861 ggaagcgcca ggtcgagccc ttcgagcgcg tcccggtacc agcccgcgac ccggcggcgc
     1921 gcctcgatct cggcggggag ccgtcgcagc ttctcggtga gcacgagggc ctgcaggttg
     1981 tcgagccgct cggggctccc gacctccgag tggacgttcg cgtcgcgctg cccgtggtga
     2041 cgcagcgagc ggatcgtcgc gccgacggac tcgtcggagc cggtcgcgaa ccccgcgtcg
     2101 ccccaggcac cgaggttctt cgtcgggtac gcgctgaacg cgccggcgtc gccgatcgag
     2161 ccggtgaccc ggccccctga cgtcgcgccg tgcgactgcg agcagtcctc caacacccgc
     2221 cacccgttgc ggcgcgcgag ctcttccacg cgcgccatgt cgaccggcag gccgtagagg
     2281 tggacgggga tgacggcgtc gacgtctgcc ccttcgagac cgtcgaggtc gatcaggtag
     2341 tcctcgtcgt tgtcgacgat caccggcgtc atcccggcga cgagcaccgc ctcgcaggtg
     2401 gcgaagaacg tgttcgcagg cagcgcgacg cgcgcccccg gcgggagatc gagtgcgcgc
     2461 agcgccatcc acagcgccga cgtgcccgac gagaccccga cggcccagcg gcacccgaac
     2521 gcctcggcgg cggcggcctc gaactcctcg agctcggggc cgagcacgaa cccgccgcgc
     2581 cgcacgaggt cctcgatccg cggtgcgagc gcatcccaga cgtcgccgtt gtcgagcctc
     2641 acagggggga tgaaatggtc gccggtcatc gggtcacctt accgacgcgt ggcctatgct
     2701 gcgcgcgatg gccggcccgc acgacgcccc gttctcgctg atcaacgacg tcgacttcgg
     2761 tgacggcgtc cgtctcgcgg ggttcgtcaa cctctacggc tgcacgatcg gggacgagac
     2821 cttcgtcggt ccgttcgtcg aggtccagcg cggcgtgacg atcggacgtc gctgcaagat
     2881 ccagagtcac accttcctct gcacgggcgt gcacatcggc gacgaggtct tcgtcgggca
     2941 cggcgtcgtc ttcacgaacg acagagcgcc gcgtgcgacg aacgccgccg gcgagatcgc
     3001 gggcgacgac gactggatgc tcgaagagac ccacgtcgga cggcgcgcgg ccatcgggag
     3061 cggagccgtc atccttcccg gcctgaccat cggggaaggc gcgaccgtcg gagccggggc
     3121 ggtggtgacg aaggacgtcc ccgcgggggc cgtcgttacc ggcgtgccgg cccggactcg
     3181 cgtctagagc gcgcgcacga cctcgatcgt gcgcgcgact gcgctgtccg ggcgaacgta
     3241 cgcctcgtaa tgacgagcgt tctcacgcat catcgcgaga cgacgatcgc gggagccgag
     3301 caggtccgca gtgcgttcga cgcattcctc cggggtctcg aactcgaggt agttctgtgg
     3361 ctgtgagaac cccggcacct gataccgcag gcgttcgctg acgattgctc tgctggcggc
     3421 gacgtactcg gcgagcttcc actgattgga ctcgtgcagg cccatcgtgg cgatccctac
     3481 gtcggctttc ttggccgccc ggaggtattg ggttcgcggg agcgcggccg catcgctgac
     3541 gcagtcgggg tagcgggccg tgccgtacgc ggtgggagcg agacctcccg agaacgacgg
     3601 cccgaaagcc tcgcggagcg cccggacgca gccggccctc atctcgttga tctgctcgcg
     3661 ctcgggcccg gcgttcccgt gcagcaaggc ctgcggatcc tcgccgggct cacccgacgg
     3721 gtcgtagacc cgcgtcatga acacgacctt cgggtcgtcg cgcacctgtg gaggggcctc
     3781 gaacactcgg acgcgagggt acgcactcct tcccatgatt gccttcgcgg ctcggctcgc
     3841 cttctgacgc agcggatcgc gcgagcgcat cgtgcggaac accgacgctc tgcacgtgac
     3901 cacgtagcta aggccgaagg ggtggatgcg gtccccctgc tggagctcgg cgttgtggcg
     3961 ctcgctgaag cttcgcttga agtaacagtc cacctccgag agaagacgct ccatctcggg
     4021 cctcggaatg ttgcgatagc cgtcgagcat gtcgtacgcg acgcggcggc cgtcgatcgt
     4081 cgcagtgagg acgttgaacg acgagaccgg ggcctcggtc gcgggaacga cgtcgaagct
     4141 cgcgtcgacc tcacgtcgct ctcccaacat ggcgaacccg gtgacgacct ggtacacgtg
     4201 ctgggcgttc ccggcgacgc gcacggagca cttcacgcga ctcactgcgc actccgccgc
     4261 gacaccctcg tgtatatatc gccccacgtc gtgacctggc tgtcgagccc gaaccgcgcg
     4321 gtggcgatgg cgctgttccg gcggcccatc gcggctgcct cggagccgtg ccggacgagg
     4381 tacgccatct tgtcggcgag ggcgcccgcg tcacctgcgg gaacgaggaa cccgttgacg
     4441 ccgtcttcga ccacctcggc cattgcgccc acgtcgctca cgatgaccgg aaggcccatc
     4501 gccatcgctt cgagcaatgc gttcggaagc ccctccagcc acgacgacgc gagcacgaaa
     4561 acgtccgagc gggccagaag gtcgagcttc gactcggtcc cgagataccc gtgccacgtc
     4621 acggcccccc ccactcccag agcgtcgcag cgccgcctca tctcgctctc gtccggtccc
     4681 gcacccgcga tgtcggcgac cacgtcgaga ccgtcgctcc gcagggctgc taccgccgcg
     4741 acgagctcgt gcacgccctt ctcctgcacg agacgtccga cgaagacgaa cgaccgcgga
     4801 agatcccgtt cgggcctttc acgccggcgc gcttcgaacg gttgcaggtc gaggaacagc
     4861 ggcacctgtt ggatcttcat ccgctcggac gcgccgagcg tcgacgagat gcttcgcgcc
     4921 tggagccgcg agaggaccac gacgaggctc gcccgctcca gcgtccgcag gacggcgcgc
     4981 cggacccacc cgtagcgcat cggcttcggg cggtccatga gccctccatg gaactggacc
     5041 accacgcgag cccccctccg agaggcgacg tggaccatcg ggagatcccg gatcaagctc
     5101 cgcgtgtcca tcgacggatt caggtggacg accgccccgg gccggcgggc gacgagcttc
     5161 cgcagctgcg cgatcttccg caacccgtcg atcagccgct tcgctcggga cgccgactct
     5221 tcgtagaacc ccacctccag atggacgagg tcgaaggctt cgttcaggcc accggacagg
     5281 agcgttctga cgtgtgtgtt gacgccgccc cacaagcgga ggcgcggccc gacgacgatc
     5341 acggtctgcc gccccgttgc tcccgctccg ctccgcccgg tgacgtcctc cagcagtgcc
     5401 tccgtccgct cgaccatcct ctcgacggtg aagtgctcct cgacgcgccg cttcgcggcg
     5461 tcgcccagcc tctttctctc tccgtcgtcc gtgatcagcc gctcgagctt gacagcaagg
     5521 gcggccgcgt cgtcgcgcgg gacgaggaag ccggtctcac ccggcaagac gatctcgtcg
     5581 accgagccga cttcggtgga gaccacgggg cgcccggcgg ccatgaactc caggaccgag
     5641 agcgggaaag cctcgccgaa cgagcacaga acgccgacgt ccgacgcagc catcactgcc
     5701 gggacgtcgg agcgcgtacc caggaagcgc acgtcgcgtt cgatgccgag ctcgcgagcc
     5761 agccgttcca gacgcggtct ctccacgccg tcaccgacga cgaggaacgt gacgcgcgcg
     5821 ccccattcga gcacctcgcg cgctgctcgc aagaacattg aatggttctt ctcgggacgc
     5881 agctgggcga cgatcgacac gacggttcgg tccggcgcga tgccgaggtc gctccgcacc
     5941 tcgtcccgag cgtccctcgc cgccgagaac gcacccacgt cgatcccgtt cgggatgacc
     6001 acgatctttc gttcgggaac ccgctccgtg gcggacacgt acgccttgtg cgcctgggat
     6061 agagcgacgc atgcgtccac gcgccggccc agcatccggt tcagcaagac cccgcgtcct
     6121 gccttctccc ggcgcgaggc gaagtggaac acggtgaccg ttgcccggac acgggccaag
     6181 acggccgccg tccaccccca cagctgcgtc aggggctggt tgttgtaata gaggacgtgg
     6241 ggagcgagcc gccggagcag cgagacgagc cggaggaacc cgcggacgtc ggccttgtgg
     6301 cgcaggacgc ggtccaccac ggtgacacct tccgagcgca gcgcctcccc gaccgggccg
     6361 gcttcgtaca gacagacgac agtcacctcg aaccttcgcc gatcgagtcg gcgcgccaag
     6421 tcggccaggg tccgctccgc gccacctacg ttgaggcttg cgttcatgac gacgacgcgg
     6481 gttgcgctca tcggcggacg cccaccaagg cacggtagag atcgactgtc tcggcggcca
     6541 cgtgcgcggg ggcaaatctc cgtgcctccg cgcgagcctt cgccgcgaga tcggtcgagt
     6601 cgccgctcag gaccccgacg atgcgctcgg ccagcgcgtc gacgtcgcgc ggcgagaaga
     6661 ggtacccatt gactccgtcc tcgatcatcc aggggacgcc gccgacggcc gacgcgacga
     6721 cggggaggcc ggcggccatg gcttccgcga cgacctga
//
LOCUS       DASQDO010000007         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_123627, whole genome shotgun sequence.
ACCESSION   DASQDO010000007 DASQDO010000000
VERSION     DASQDO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123627"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VEV43_00170"
     CDS             complement(<1..612)
                     /locus_tag="VEV43_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21968.1"
                     /translation="MRRLVGSLVCVLLLMGGATVSSAQTPGPCRWVTDSTTVRDAVLF
                     GVAAPESTNVFAVGARATAQDRAVTLVRHWDGSAWETQPSPNVNRQSNFLNDVAVAPD
                     GTAWAVGERSRLKSKTVVQRYDGTTWRMHQSLNPSEDLNALHGVDVAPSGDVYAAGAR
                     WNAKRRYRTMVHRYDGKAWTMAAKKFPGIFWDLDVVAEDDVWAV"
     gene            696..>1695
                     /gene="ald"
                     /locus_tag="VEV43_00175"
     CDS             696..>1695
                     /gene="ald"
                     /locus_tag="VEV43_00175"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYP21969.1"
                     /translation="MRVGIPRELKDNEYRVAITPAGVRELVVGGHEVFIEHDAGRGSS
                     ITNDEFERAGAQILPDADATFAEAEMILKVKEPVPEEFHRLHDGLLLFTYLHLAASEP
                     VTRALIDSGTTGVAYETVELPDRSLPLLAPMSEVAGRMAPQVGAISLQREHGGRGVLL
                     GGVSGVRPGRVVVIGAGMSGANAAWIAQGMEAEVVILDKNINKLREVDRIHQGKILTL
                     ASNRLAVEETVTQADLVIGAVLVPGAVAPKIVSEDVVREMKPGAVIVDISIDQGGCFE
                     TSHMTTHSDPTYVVHDVVHYCVGNMPGAVPHTSTYALTNATMPYVIDLADKGLEGAV"
BASE COUNT          271 a          572 c          597 g          255 t
ORIGIN      
        1 caccgcccag acgtcgtcct ccgcgacgac gtcgaggtcc cagaagatgc cggggaactt
       61 cttcgcggcc atcgtccagg ccttgccgtc gtatcggtgg accatcgtcc ggtatctccg
      121 cttggcgttc catcgggcac cggcggcgta gacgtcaccc gacggagcga cgtcgacgcc
      181 gtggagcgcg ttcaggtcct cgctggggtt caggctttgg tgcatccgcc aggtcgtacc
      241 gtcgtagcgc tgcacgaccg tcttcgactt gaggcggctg cgctcgccga cggcccaggc
      301 ggtcccgtcc ggagcgacgg cgacgtcgtt gaggaagttc gactggcggt tgacgttcgg
      361 gctcggctgg gtctcccacg ccgagccgtc ccaatggcgc acgagcgtca ctgcgcgatc
      421 ctgcgcggtc gcccgggcgc ccaccgcgaa cacgttcgtg gactcggggg cggcgacgcc
      481 gaagagaacc gcgtccctga cggtggtcga atcggtgacc caccggcacg gccccggcgt
      541 ctgggcgctc gagaccgtcg cgccgcccat gagcagcagc acgcagacga ggctccccac
      601 cagccgtctc atcggagaag cgtagtcgag aaggcggtca caaagctagg taggtgaacc
      661 cggcccgttc gaccgaggag ggtaaggttc gggtcatgag agtcgggatc ccgcgcgagc
      721 tgaaggacaa cgagtaccgc gtcgcgatca ctcccgccgg cgtgcgcgag ctcgtggtcg
      781 gcgggcacga ggtcttcatc gagcacgacg cgggccgagg ctccagcatc accaacgacg
      841 agttcgagcg cgccggcgcc cagatcctgc ccgacgcgga cgcgacgttc gccgaggccg
      901 agatgatcct caaggtcaag gagccggtgc cggaggagtt ccaccggctg cacgacgggt
      961 tgctcctgtt cacatacctc cacctcgccg cgagcgagcc ggtgacccgg gctctgatcg
     1021 actccggcac gaccggcgtc gcctacgaga ccgtcgagct tcccgaccgc tcgctgccgt
     1081 tgctagcgcc gatgagcgag gtggcgggcc gtatggcgcc tcaggtcggt gcgatatcgc
     1141 tccagcggga gcacgggggc cgcggggtcc tgctcggcgg tgtctccggt gtccggccgg
     1201 gccgcgtcgt ggtgatcggg gccgggatgt ccggcgcgaa cgcggcttgg atcgcgcagg
     1261 gcatggaggc cgaggtcgtg atcctcgaca agaacatcaa caagctccgc gaggtcgacc
     1321 gcatccacca gggcaagatc ctcacgctcg cgtcgaaccg gctcgcggtc gaggagaccg
     1381 tgacgcaggc cgacctcgtc atcggtgccg tgctcgtccc gggggcggtc gcgccgaaga
     1441 tcgtcagcga ggacgtcgta cgcgagatga agcccggcgc cgtcatcgtc gacatctcga
     1501 tcgaccaggg cgggtgcttc gagacctcgc acatgacgac gcactcggac ccgacgtacg
     1561 tcgtgcacga cgtcgtccac tactgcgtcg ggaacatgcc gggcgcggtg ccgcacacgt
     1621 cgacgtacgc gctgacgaac gcgacgatgc cgtacgtgat cgacctcgcg gacaaggggt
     1681 tggaaggcgc cgtgc
//
LOCUS       DASQDO010000008         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_123841, whole genome shotgun sequence.
ACCESSION   DASQDO010000008 DASQDO010000000
VERSION     DASQDO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123841"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VEV43_00180"
     CDS             <1..483
                     /locus_tag="VEV43_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HYP21970.1"
                     /translation="PHVATGDMLRAAVEDESELGERVRPILASGHLVPDDLMIELIRQ
                     RLHDDDTGNGFVLDGFPRTFVQARALDDMLGELDRALALVLEFQLPEEMARERLLGRG
                     RSDDNEETIRTRLERQRVPEELVAYYRTKGILVGIHADGTVDEVFAEVQDVLATAAAR
                     "
     gene            480..1244
                     /gene="map"
                     /locus_tag="VEV43_00185"
     CDS             480..1244
                     /gene="map"
                     /locus_tag="VEV43_00185"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HYP21971.1"
                     /translation="MIIRKSPEEIEAMARSGRVVAEALAAAGDAIAPGVTTRELDEIA
                     EELIRAHGGVPTFKGYRGFPASICASPNSMVVHGIPGPYRLEEGDVISIDVGVTLDGF
                     VADSAFTFPVGTADPEAHRLLEVCQAALDAGIEAARPGNRVGAISAAVQQATEAAGFS
                     VVRSLVGHGVGRSMHEEPQVPNHGEPSDGPVLEPGTTIAIEPMITAGSEEVFVARDGW
                     SISTRDDSLAAHFEHTVAVLEDGPRILTAAAVREPA"
     gene            1324..1437
                     /gene="rpmJ"
                     /locus_tag="VEV43_00190"
     CDS             1324..1437
                     /gene="rpmJ"
                     /locus_tag="VEV43_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYP21972.1"
                     /translation="MKVRPSVKPMCDRCRVIKRHGRVLVICTNPRHKQRQG"
     gene            1439..>1512
                     /gene="rpsM"
                     /locus_tag="VEV43_00195"
     CDS             1439..>1512
                     /gene="rpsM"
                     /locus_tag="VEV43_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530968.1"
                     /note="located at the top of the head of the 30S subunit,
                     it contacts several helices of the 16S rRNA; makes contact
                     with the large subunit via RNA-protein interactions and
                     via protein-protein interactions with L5; contacts P-site
                     tRNA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYP21973.1"
                     /translation="MARIAGVNLPREKRLEIALTYIYG"
BASE COUNT          231 a          513 c          557 g          211 t
ORIGIN      
        1 ccgcacgtcg cgacgggaga catgctccgg gccgccgtcg aggacgagag cgagctcggc
       61 gagcgggtgc ggccgatcct cgcgagcggg catctcgtcc ccgacgacct gatgatcgag
      121 ctgatccgcc agcgcctgca cgacgacgac acgggaaacg gcttcgtgct cgacggattc
      181 ccgcgcacgt tcgtgcaggc gagggcgctc gacgacatgc tcggggagct cgaccgcgcg
      241 ctcgcgctcg tgctcgagtt ccagctcccg gaggagatgg cgcgcgagcg gcttctcggc
      301 cgcggacgaa gcgacgacaa cgaggagacg atccggacgc ggctcgagag acagcgcgtc
      361 ccggaggagc tcgtcgcgta ctacaggacg aaggggattc tcgtcggcat ccacgccgac
      421 ggcaccgtgg acgaggtctt cgccgaggtg caggacgtgc tcgcgaccgc cgccgcgcga
      481 tgatcatcag gaagtcgccg gaggagatcg aggcgatggc gcgctccgga cgcgtggtcg
      541 ccgaggccct cgccgctgcg ggggacgcga tcgcgccggg cgtgacgacg cgcgagctgg
      601 acgagatcgc ggaggagctg atccgcgcgc acggcggcgt cccgaccttc aagggctacc
      661 ggggcttccc ggcgtcaatc tgcgcttcgc cgaactcgat ggtggtgcac ggcatccccg
      721 ggccctaccg gctcgaggag ggcgacgtga tctcgatcga cgtcggcgtg acgctcgacg
      781 ggttcgtggc ggactcggcc ttcacgttcc ccgtcgggac ggccgacccg gaggcgcacc
      841 ggctgctaga ggtgtgccag gcggccctcg acgccgggat cgaggcggcg cggccgggga
      901 atcgggtggg ggcgatctcc gctgcggtac agcaggccac ggaggcggcg ggcttctccg
      961 tcgtccgctc gctcgtcgga catggcgtcg ggcggtcgat gcacgaggag ccccaggtgc
     1021 cgaaccacgg cgagccgagc gacggcccgg tgctcgagcc cgggacgacg atcgcgatcg
     1081 agcccatgat caccgcgggc tcggaggagg tcttcgtggc ccgcgacggg tggtcgatct
     1141 cgacccgcga cgactcgctc gcggcccact tcgagcacac cgtcgcggtg ctcgaggacg
     1201 gcccgcggat cctcaccgcg gctgcggttc gggagcccgc gtagccttgg gcggaagcgg
     1261 ccttctggta cgattcaccg gtcgcggcgc cgccggcctt cggcgtgccg cagcccatct
     1321 ctcatgaaag tgcgtccgtc cgtcaagccc atgtgcgacc gctgccgcgt gatcaagcgg
     1381 cacggacgcg tcctggtcat ctgcacgaac cctcggcaca agcaaaggca gggataagtt
     1441 ggctcgtatc gccggtgtga acctcccccg cgagaagcgg ctggagatcg cgctcaccta
     1501 catctacggc at
//
LOCUS       DASQDO010000009         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1102626, whole genome shotgun sequence.
ACCESSION   DASQDO010000009 DASQDO010000000
VERSION     DASQDO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102626"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..859)
                     /locus_tag="VEV43_00200"
     CDS             complement(71..859)
                     /locus_tag="VEV43_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYP21974.1"
                     /translation="MTEYESLTWDLADHVATVTLNRPEKKNAMSWTMFEEIRAVFDRA
                     SADDDVRCLVVTGAGGAFCSGADLSDPANLVDSVFAFKERMRTIHAIASAVVDCTVPT
                     IAKVTGIAAGAGCNLAFCCDLVVATRDAAFAELFVKRGLVVDFGGSWALSRLLPLNRA
                     KELALLGDTLSAEEAERWGLVNRLCEGAEVDQVVKDLAARLVSLPPRTVSMIKENLNR
                     AGHRSLDENLDAESLTQSLAFGSEDTREALMAFVEKREPRFQGR"
     gene            complement(868..1221)
                     /locus_tag="VEV43_00205"
     CDS             complement(868..1221)
                     /locus_tag="VEV43_00205"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYP21975.1"
                     /translation="MHARSMLGRRGEDAACDLYRRLGYRVVERNYRAGRGEVDVVARR
                     GDTVVFCEVKTRRSDEWGVPAEAVDRGKQARLRKLAAAWLAERRPGYVDVRFDVVSVI
                     VRGDRADVTHVADAF"
     gene            complement(1259..1588)
                     /locus_tag="VEV43_00210"
     CDS             complement(1259..1588)
                     /locus_tag="VEV43_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HYP21976.1"
                     /translation="MSVEDLEKYEAEMELQLYKEYRDVLPMFKFVVETERRFYLANGV
                     NVDTRHVGAKVWFEVELTDAWVWDMHRPARFVSHVKVVTFRDLNVEELAQLAERDIIP
                     DPPGMAH"
     gene            complement(1585..>2290)
                     /locus_tag="VEV43_00215"
     CDS             complement(1585..>2290)
                     /locus_tag="VEV43_00215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYP21977.1"
                     /translation="VRGMVTDASGLLEARLAGAGFERVAGADEVGRGALAGPLMAAAV
                     ILPPGTCIEGLRDSKMCTALQRERLAGRIKEVAISYCVVRVSPEKIDRTGLQRCNLEA
                     LRRALKGLGEEPDYVLLDAFRLRRAAWPTLGVKKADAVSTNVAAASILAKVERDRAMK
                     RYHRRHPEYGFATNVGYGTREHWRALQQYGPCTIHRRSFYGVEGFVDADGVFRPHLAR
                     DLDDDGYDDQHRQETG"
BASE COUNT          352 a          819 c          777 g          342 t
ORIGIN      
        1 tggtcatgtc caccccccac ccgggaaccg tgggctcact caacccgggg cggcgatgga
       61 agtaaaggac ctaacggccc tggaagcgcg gctccctctt ctcgacgaag gccatcagcg
      121 cctcgcgggt gtcctcgctc ccgaaggcga gggattgcgt cagggactcg gcgtccaggt
      181 tctcgtccag cgagcggtgg ccggcacggt tgaggttctc cttgatcatc gagaccgtcc
      241 gggggggcag ggacacgagg cgggcggcga ggtccttcac gacctggtcg acctcggccc
      301 cctcgcagag ccggttcacg aggccccacc gttcggcttc ctccgcggag agcgtgtcgc
      361 cgagcagcgc cagctccttc gcacgattca gcggcagcag ccggctcagc gcccaggagc
      421 cgccgaagtc gacgacgagc ccccgcttga cgaacagctc ggcgaaagcg gcgtcgcgcg
      481 tggcgacgac caggtcgcag cagaaagcga ggttgcaccc ggccccggcg gcgatgcccg
      541 tgaccttcgc gatcgtcggg acggtgcagt cgacgaccgc ggacgcgatc gcgtggatcg
      601 tgcgcatgcg ctccttgaag gcgaagacgg agtcgacgag gttcgcgggg tcggagaggt
      661 ccgcgcccga gcagaacgca ccccccgcgc cggtcacgac gaggcagcgc acgtcgtcgt
      721 cggccgaggc ccggtcgaac accgcgcgga tctcctcgaa catcgtccac gacatcgcgt
      781 tcttcttctc ggggcggttc agcgtcaccg tcgcgacgtg atccgcgagg tcccacgtca
      841 gcgactcgta ttcggtcacg gcggctccta gaaggcgtcg gcgacgtgcg ttacgtcggc
      901 tcgatcgccg cgcacgataa ccgagacgac gtcgaaccgg acgtcgacgt agccggggcg
      961 gcgctcggcc agccacgccg cggcgagctt ccgcagacgc gcctgcttgc cccggtcgac
     1021 ggcctcggcc gggacccccc actcgtcgct ccgccgcgtc ttgacctcgc agaacacgac
     1081 cgtgtcgccg cgccgcgcta cgacgtcgac ctcgcccctg cccgcgcggt agttccgttc
     1141 gacgacgcgg tagcccaggc ggcgatagag atcgcaggcg gcgtcctcgc cgcggcggcc
     1201 gagcatcgag cgcgcatgca tgcgctcaac ctaagaaggg ggtgtgacga aaagcgcgct
     1261 agtgggccat cccgggcggg tccgggatga tgtcgcgctc cgcgagctgc gccagctcct
     1321 ctacgttcag gtcgcggaac gtcacgacct tgacgtggga gacgaagcgg gccgggcggt
     1381 gcatgtccca gacccacgcg tcggtgagct cgacctcgaa ccacaccttc gcgccgacgt
     1441 ggcgcgtgtc gacgttgacg ccgttcgcca gatagaagcg ccgttcggtc tcgacgacga
     1501 acttgaacat cgggaggacg tcccggtact ccttgtagag ctgcaactcc atctcggcct
     1561 cgtacttctc gaggtcctcg acgctcatcc cgtctcctgt ctgtgctggt cgtcgtagcc
     1621 gtcgtcgtcc aagtctcgcg ccagatgcgg ccgaaagacg ccatcggcgt cgacgaatcc
     1681 ctcgaccccg tagaacgacc ggcggtggat ggtgcacggc ccgtattgct ggagcgcgcg
     1741 ccagtgctcg cgtgtcccgt aaccgacatt cgtcgcaaac ccgtactcgg ggtggcggcg
     1801 gtggtagcgc ttcatggccc ggtcacgctc gaccttcgcc aggatcgacg ccgcggcgac
     1861 gttggtcgag acggcgtcgg ccttcttcac gcccagcgtc ggccacgccg cgcgccgcag
     1921 ccggaacgcg tcgagcagca cgtagtcggg ctcctcgccg aggcccttga gcgcacgccg
     1981 caaagcctcg aggttgcacc gttggaggcc ggtgcggtcg atcttctcgg ggctgacccg
     2041 cacgacgcag taggagatcg cgacctcctt gatccgcccg gcgaggcgct cgcgctggag
     2101 cgcggtgcac atcttggagt cccgcaggcc ctcgatgcag gtcccgggcg gaaggatcac
     2161 ggccgccgcc atcaggggcc cggcgagggc gccccggccg acctcgtcgg cgccggcgac
     2221 ccgctcgaag ccggctccag ccaggcgggc ctcgaggagc ccgctcgcgt ccgtaaccat
     2281 cccccgcaca
//
LOCUS       DASQDO010000010         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_307543, whole genome shotgun sequence.
ACCESSION   DASQDO010000010 DASQDO010000000
VERSION     DASQDO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307543"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /gene="uvrB"
                     /locus_tag="VEV43_00220"
     CDS             <1..1754
                     /gene="uvrB"
                     /locus_tag="VEV43_00220"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HYP21978.1"
                     /translation="DDLDRLRLAATSNLVSRRDVLLVASVSCIFGLGSPESYKNSVVP
                     IRKDVATDRDEMLRKLLDLQYARNDIDFKRATFRVRGDVVELHPSYDEFAYRIEFFGD
                     EITSIDAINPLTGELLASHDQVFIYPAVHYVMPEDRVAGAVQSIREELDLQVMKLRGE
                     GKLLEAQRLIARTKYDLEMILEVGYCSGIENYSRHLDGRQPGEKPYTLIDYFPKDFLL
                     VIDESHVTLPQIKAMYNGDRMRKTVLVDHGFRLPSAMDNRPLKYEEFEEMWRQVVFVS
                     ATPGKIELEKTGGEVVEQVIRPTGLVDPEIIVHPAQGQVPHLLGAIKERVERGERVLV
                     TALTKRLAEDLSAYIQEAGIRGRYLHSEIQTLERVEILSDLRKGEFDVLVGINLLREG
                     LDLPEVSMVAILDADKAGFLRSETSLIQTIGRAARNVNAKVILYADTVTPAMQKAMEE
                     TERRRVLQLKYNAEQGITPQTVKKAIRTSIESEMKARKTVQDAIHANDESLDQTEIVK
                     LLEEEMLEAARNLEFERAAQLRDKLNEMKGAPTIKSGTQFAPMPAGDGSDGDGDGEQR
                     KIWQPKTPKRGGRKAAR"
     gene            1896..2795
                     /gene="nadC"
                     /locus_tag="VEV43_00225"
     CDS             1896..2795
                     /gene="nadC"
                     /locus_tag="VEV43_00225"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HYP21979.1"
                     /translation="MYPFPTYEQLKDLIQLAKREDLATDDVTSRLMVREDHVGVGTFV
                     QKEVGVVCGLPVVEMVCRAYDERLRVEMIPGFHMEIIEGRFSDAKRTPLLRVRGPMRS
                     LLSAERVALNFLQHMSGVATHTHRFARRVAGTGAKIYDTRKTLPGHRLLDKYAVRCGG
                     GFNHRVGLYDGLLVKDNHLAHVPLRELAGYLANVVNQCRSEDPTRLVEIEVDGIEQLR
                     EVLKVDGVHVILLDNMDCPAMESAVQLRNGAGRKGQVELEASGGVTLETVRSIAATGV
                     ERIAVGAITHSAPALDVGLDVED"
BASE COUNT          693 a         1317 c         1343 g          511 t
ORIGIN      
        1 acgacgacct cgaccgcctg cggctggccg ccaccagcaa cctggtgagc cgccgggacg
       61 tgctgctggt ggcgtcggtg agctgcatct tcggcttagg ctcgcccgag tcgtacaaga
      121 acagcgtcgt gccgatccgc aaggacgtcg ccaccgaccg ggacgagatg ctgcgcaagc
      181 tgctcgacct gcagtacgcc cgcaacgaca tcgacttcaa gcgcgcgacg ttccgcgtcc
      241 gcggcgacgt ggtcgagctg cacccgtcgt acgacgagtt cgcctaccgc atcgagttct
      301 tcggggacga gatcacgagc atcgacgcga tcaacccgct gaccggcgag ctgctcgcca
      361 gccacgacca ggtgttcatc tacccggccg tccactacgt gatgccggag gaccgggtgg
      421 cgggcgcggt gcagagcatc cgggaggagc tggacctcca ggtgatgaag ctccgcggcg
      481 agggcaagct gctcgaggcg cagcggctca tcgcgcggac gaagtacgac ctggagatga
      541 tcctggaggt cggctactgc tccggcatcg agaactacag ccgccacctc gacggccgcc
      601 agcccggcga gaagccgtac acgctgatcg actacttccc gaaggacttc ctgctcgtga
      661 tcgacgagag ccacgtgacg ctcccgcaga tcaaggcgat gtacaacggc gaccggatgc
      721 gcaagacggt gctcgtcgac cacggcttcc gcctgccgag cgcgatggac aaccggccgc
      781 tgaagtacga ggagttcgag gagatgtggc ggcaggtcgt gttcgtctcg gccacgccgg
      841 gcaagatcga gctggagaag acgggcggcg aggtcgtcga gcaggtgatc cgcccgacgg
      901 ggctcgtcga cccggagatc atcgtccacc ccgcccaggg ccaggtgccg cacctgctcg
      961 gcgcgatcaa ggaacgcgtg gaacgcggcg agcgcgtgct cgtaacggcc ctgacgaaac
     1021 gcctcgccga ggacctctcg gcctacatcc aggaggccgg catccgcggg cgctacctgc
     1081 acagcgagat ccagacgctg gagcgggtgg agatcctcag cgacctccgc aagggcgagt
     1141 ttgacgtgct cgtcggcatc aacctgctgc gcgagggact ggacctgccg gaggtgtcga
     1201 tggtcgcgat cctcgacgcc gacaaggcgg gtttcctgcg cagcgagacg tcgctcatcc
     1261 agacgatcgg ccgggccgcg cgcaacgtga acgcgaaggt gatcctgtac gccgacacgg
     1321 tcacgcccgc catgcagaaa gcgatggagg agacggagcg gcgtcgcgtg ttgcagctga
     1381 agtacaacgc cgagcagggc atcacgccgc agacggtgaa gaaggcgatc cgcacgagca
     1441 tcgagtcgga gatgaaggcg cgcaagacgg tgcaggacgc gatccacgcc aacgacgagt
     1501 cgctcgacca gaccgagatc gtcaagctcc tggaagagga gatgctcgag gccgcccgga
     1561 acctcgaatt cgaacgtgcc gcgcagctgc gcgacaagct caacgagatg aagggcgccc
     1621 cgaccatcaa gagcggcacc cagttcgccc cgatgcccgc cggcgacggc agcgacggag
     1681 acggagacgg cgagcagcgc aagatctggc agcccaagac ccccaagcgg ggcggccgca
     1741 aggcggcccg ctagtctccc cgccgtggcc cgggcatctc gcccgcgtgg tccagcaaga
     1801 agaagaacgc gggcgggacg cccgcgccgc cggggggatc acggccctga cccacacttc
     1861 gcgccgtgcg tagaatgggt ctggagagca gtcccatgta ccccttcccc acctacgagc
     1921 agctcaagga cctgatccag ctcgccaagc gcgaggacct cgccaccgac gacgtcacga
     1981 gccggctcat ggtgcgcgag gaccacgtcg gcgtcggcac gttcgtgcag aaggaggtcg
     2041 gcgtcgtctg cgggttgccg gtggtcgaga tggtctgccg ggcgtacgac gagcggctgc
     2101 gcgtcgagat gatccccggg ttccacatgg agatcatcga ggggcgcttc agcgacgcga
     2161 agcgcacgcc gctgctgcgc gtgcgcggtc cgatgcgctc gctgctgtcg gcggagcgcg
     2221 tcgcgctgaa cttcctccag cacatgtccg gcgtcgccac ccacacgcac cgcttcgccc
     2281 ggcgcgtggc cggcacgggc gcgaagatct acgacacgcg caagaccctc cccggccacc
     2341 ggctgctcga caagtacgcc gtccgctgcg gcggggggtt caaccaccgc gtcgggctct
     2401 acgacggcct gctcgtgaag gacaaccacc tcgcgcacgt ccccctgcgg gaactcgcgg
     2461 gctacctcgc caacgtcgtc aaccagtgcc gctccgaaga cccgacacgc ctcgtcgaga
     2521 tcgaagtaga cggcatcgag cagttgcgcg aggtgctgaa ggtcgacggc gtccacgtga
     2581 tcctgctcga caacatggac tgccccgcga tggagtccgc cgtccagctc cgcaacgggg
     2641 cggggcgcaa gggccaggtg gagctcgagg cgtcaggtgg ggtgacgctc gaaacggtcc
     2701 ggtcgatcgc cgcgacgggc gtggagcgca tcgccgtggg ggcgatcacc cactcggcgc
     2761 cggcgctcga cgtcgggctg gacgtcgagg actgaaccct tcgtcgatgt gacgacgtcc
     2821 cgaccggctt gtgggcgcgc ttcacgttat cggagcctct gcacgatttc tcgggaatcc
     2881 ccagctccgc gtttcccgca ttccaccatt ccgcccgatt tgcctgcctc cggtctgaat
     2941 cgtacatcaa ccacgtccgg cccgcttggt aagcgggccg gcggatgagg atccccgaaa
     3001 gggcgaagcc gccgcaaggc ggcgcccggg ccgcgacgcg agaagcgcgt ggcccggctc
     3061 gtcggagacg cccgtgcgag cgatcgcacg atcgaagccc ggcgatgcgc aaagcgcccg
     3121 gtcccgctca cgcggcgacg gcgcgcgtcc ggcaacggac gcgcccgcgg cccgcgcgag
     3181 acggatcgag ggcgctgccg aaaggatgct cgtgaatgcc tccgtgtgcc gtggtcgtcc
     3241 cgtggacgga agtccccgcg gcgcaacccc ggtgcccggc acgtcacgcg ctgcttatcg
     3301 gtcctcctct ccccgtgtcg gtgctgcgtc cgtccacgct gccgtccgcg cgacccgtgc
     3361 aaacgggcgt tgtgaacggg ggacgaatgg gaaagggcga tcaagtcggc atcgcccgcc
     3421 gccgatgcaa gcactggcac gacgcggccc ccgcgtgagc ggaacggtcg cggtgcgacg
     3481 cccggtcggt cgccgtaagg cggcaggccg ccagcggccc ggtggtgacg agcgaggaac
     3541 gcgtgttccg cgcggccggc catcgggctc caggatcacc aggcgccggc gcgccagacg
     3601 ggcgcgaaag gagacggctt cacgacccgc ccaccggccg cgaggcagct cgcggttgac
     3661 gtgtcgagtg gaacgcaacg ggcccgagtg tacagacgcg aggccccgcg cgacggcagt
     3721 gacacagtga ccgagcagag agagagtgac cgcgggccgc gggaccagcg acgcaacgtg
     3781 cgaagcggtc ggcggcgccg cagacagagg accggatttt cggaggcatc taccatgacc
     3841 gcgtttcgca agtcgtccaa caag
//
LOCUS       DASQDO010000011         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_919590, whole genome shotgun sequence.
ACCESSION   DASQDO010000011 DASQDO010000000
VERSION     DASQDO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919590"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VEV43_00230"
     CDS             complement(<1..1074)
                     /locus_tag="VEV43_00230"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HYP21980.1"
                     /translation="MSVRAICEASAAAAPAVAALPGRVKDAALHAMADALLASEADLV
                     AANGVDVANARAEGTSSTLVDRLTLTSSRLESMAEGLRSVAAQPDPVGEIVAGWTRPN
                     GLLIEQVRVPLGVVAVIYEARPNVTADVSGLCLKSGNACVLRGSSIALRSNQAIVDVL
                     RAAVSSAGVPQDAIQLVPDTTREGATELMQAKGLVDLLVPRGGRALIESIEQNATVPY
                     IVDGDGNCHVYVDRAADSSMAREIVLNAKTQRPSVCNAAETLLVHAELAKEWLPETLN
                     ALRERGVDVRGDDEVRALWPDAAEATEDDWGTEYLDYKIAVRVVGSLDEAIAHVNRWG
                     TSNAEAIVTEDLAAARRFTAEVDS"
     gene            complement(1078..>1709)
                     /gene="proB"
                     /locus_tag="VEV43_00235"
     CDS             complement(1078..>1709)
                     /gene="proB"
                     /locus_tag="VEV43_00235"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYP21981.1"
                     /translation="LVANVAHARLLVLLTNARGLHTADPRRNPDAPVIDEVERITPEL
                     ERAAGGRGSNLASGGMTSKIAAAWVATFSGVAVVVADATEPDVLGRIARGEPLGTYFH
                     PRPARATAKRLWIAFARPPTGTVTVDAGARKALVEDKRSLLPAGVTSVSGEFSAGDAV
                     DVGDTSGAVFARGLVRFSAAELDAARGRPTAEFEGREVIHRDQLVVLED"
BASE COUNT          229 a          611 c          628 g          241 t
ORIGIN      
        1 cgagtcgacc tcggccgtga acctgcgggc cgcggcgagg tcctcggtca cgatcgcctc
       61 ggcgttcgag gtgccccacc ggttcacgtg cgcgatcgcc tcgtcgagag agccgacgac
      121 ccggaccgcg atcttgtagt cgaggtactc ggtgccccag tcatcctcgg tcgcctcggc
      181 cgcgtcgggc cacagcgcgc gcacctcgtc gtcgccgcgc acgtcgacgc cgcgctcgcg
      241 cagcgcgttg agggtctccg ggagccactc cttcgcgagc tccgcgtgga cgagcagcgt
      301 ctcggccgcg ttgcagacgc tcggtcgttg ggtcttcgcg ttgaggacga tctcgcgcgc
      361 catcgacgag tcggcggcgc ggtcgacgta cacgtggcag ttgccgtcgc cgtcgacgat
      421 gtacgggacg gtggcgttct gttcgatgct ctcgatcagc gcacggcccc cgcgcggcac
      481 gagcaggtcg acgaggccct tcgcctgcat cagctcagtg gcgccctcgc gggtggtgtc
      541 ggggacgagc tggatcgcgt cctgcgggac tccggccgac gacaccgccg cgcgcagcac
      601 gtcgacgatc gcctggttcg agcgcaacgc gatcgacgag ccgcgcagca cgcacgcgtt
      661 gccggacttc agacagagcc ccgacacgtc cgcggtgacg ttggggcgcg cctcgtagat
      721 cacggcgacc acaccgagcg gcacgcggac ctgttcgatc aacagaccgt tgggccgggt
      781 ccaccccgcg actatctccc cgacggggtc gggctgagcc gcgaccgagc gcagtccctc
      841 ggccatcgac tcgagccggc tcgaagtcag agtgagccgg tcgacgagcg tcgacgacgt
      901 cccttccgcg cgggcgttgg cgacatcgac cccgttcgcc gcgaccaggt cggcttcgga
      961 ggcgagcaga gcgtcggcca tcgcgtggag ggccgcgtcc ttgactctgc cggggagcgc
     1021 cgcgacggcg ggggccgcgg ccgccgaggc ttcgcagatg gcgcggacgc tcaccggtca
     1081 gtcctccaac acgacgagct gatctcggtg tatgacctcg cgcccttcga actcggcagt
     1141 gggacggccg cgggcggcgt cgagctccgc ggcggagaag cgtaccaacc cgcgtgcgaa
     1201 caccgccccc gacgtgtcgc cgacgtcgac ggcgtcaccg gccgaaaact cgcccgagac
     1261 cgacgtgact cctgcgggga ggaggctgcg cttgtcctcc acgagcgcct tgcgggcgcc
     1321 cgcgtcgacc gtcaccgtgc cggtcggagg ccgcgcgaac gcgatccaca gacgcttcgc
     1381 ggtcgcccgc gccggacgcg ggtggaagta ggtgccgagg ggttcgccgc gggcgatccg
     1441 gccgagcacg tcgggctcgg tggcgtcggc gactacgacg gcgacgcccg agaacgtcgc
     1501 gacccatgcc gcggcgatct tcgaggtcat gcccccgctg gcgaggttcg agccgcggcc
     1561 ccccgcggcg cgctcgagct cgggcgtgat gcgctcgacc tcgtcgatca cgggggcgtc
     1621 ggggttgcgg cgcgggtcgg cggtgtggag gccgcgcgcg ttcgtgagca gcacgaggag
     1681 ccgggcgtgc gcgacgttcg ccacgagcg
//
LOCUS       DASQDO010000012         6366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_307776, whole genome shotgun sequence.
ACCESSION   DASQDO010000012 DASQDO010000000
VERSION     DASQDO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307776"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VEV43_00240"
     CDS             complement(<1..642)
                     /locus_tag="VEV43_00240"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HYP21982.1"
                     /translation="MNLALPFKLGIDPLEWGITPTIDIGALSVSPHGIGIAIGYLVGA
                     QLMVRRARRFDGPPENDIWNALFYALIGAIVGARLGYVLGHFSEVTDGGDDLLGVFKI
                     YEGGISLLGGITGAVLAALPYAIRKKMGFWRSTDLAVPGLALGIVIGRIGDLVIGDHL
                     GDPTTFALGWRCTGETGGVEPASAATYERLAESSPPSLGCFDVVLHQTALYDFF"
     gene            complement(639..1352)
                     /gene="thyX"
                     /locus_tag="VEV43_00245"
     CDS             complement(639..1352)
                     /gene="thyX"
                     /locus_tag="VEV43_00245"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HYP21983.1"
                     /translation="MSDDQIRPGDKPHELLLFDHGFVRLDDAMASDLSVVNSARVSFA
                     VRREEMDDRDKGLIKFLMRERHGTPFEHNSFRFHIRTPIFVAREWFRHRVGSFNEESG
                     RYHKLSDDFYVPAPEAVRTQVGKPGSYSFESVDEPLADETIDTFKRIYKDLFAEYEGM
                     IQKGVAKELARAILPFGIYTQFYWTLNARSLMNFLSLRNAAAAQYEIRVYADAVERLF
                     AERMPITHECFVEFGRRAP"
     gene            complement(1349..1915)
                     /locus_tag="VEV43_00250"
     CDS             complement(1349..1915)
                     /locus_tag="VEV43_00250"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HYP21984.1"
                     /translation="MPDDPTPTPAPAGDIESVPEVPAQSELAKPRPKGEPEAVVALRE
                     MIETKLGSLGGYLVDNHGSYVVGMQSARTFVVPTWLENGMTVVRVFAITNLDVPVTAE
                     LTRWLLQRNLEFVFGAFALDVDNGAVWFNHNLLGGYAAPEELEATIGAVIETADRFDD
                     EIKSQFGGRLYVEAEGQTIAPPAAPGYL"
     gene            1938..3620
                     /gene="hutU"
                     /locus_tag="VEV43_00255"
     CDS             1938..3620
                     /gene="hutU"
                     /locus_tag="VEV43_00255"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYP21985.1"
                     /translation="MPEQPDPIRPGTPAPRGPHISCKGWPQEAALRMLANNLDPEVAE
                     RPEDLVVYGGSGRAARSVEAFDAIVRELRRLGDDETLLVQSGKPVAVFRTHARAPRVL
                     ISNSMLVPAWATWDEFHRLEAMGLTMYGQMTAGSWIYIGTQGILQGTYETLAELAARN
                     FGGSLAGRIVLTAGLGGMGGAQPLAVTMNEGVALCVEVDPARIERRLKTRYVDRSTDD
                     VDEAKAWAEAARDERRPESIARLGNAAEVLPALLEAGFAPDVVTDQTSAHDLLDGYVP
                     AGLPLDEATRLRRTDPDTYVKRSLEAVAAHVEAWLGFQERGAICVEYGNNIRNQARTA
                     GVERAFEIPGFVEAYVRPLFCEGKGPFRWAALSGDPADIAVTDEAILDLFPDDDRLHR
                     WIRMASERVAFQGLPARICWLGYGERAKAGLAFNELVRSGRVSAPIVIGRDHLDAGSV
                     ASPYRETEGMIDGSDAIADWPILNALLNTAAGAHWVSVHHGGGVGIGYSIHAGMVVVA
                     DGTDDAAERLERVLDTDPGMGVVRHVDAGYSRAVEVASERGVHVPMEALARD"
     gene            complement(3617..4927)
                     /locus_tag="VEV43_00260"
     CDS             complement(3617..4927)
                     /locus_tag="VEV43_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYP21986.1"
                     /translation="MTTKAITGALVLTEDGFTGDVAVVVEGDTIADVVGRDSVEAVDW
                     GDVAIVPGTVNAHGHAFQNLLKGWADDRTFDSWRDDVLYPFSEDLDAGDVYTGALFAF
                     TEALRAGVTTTVDFFYLHDDGNDNAEQVIRAARDAGIGLVLARALYDADAPTKAPARY
                     REKTGDSVDRCRALATAHAGDPMVSVQPAPHSLHAASPETIAAALELAEELDVPCHLH
                     LAEARYERDQVRERYGTTPVRLLAREGLLGPRLVTIHTVWADDEELDLLAEAGAAVVH
                     CPGANACLGDGIARLPEMLSRGIRVALGPDGGCANNRQSVFEEMRQATLMAKARLTDG
                     SAVSAETAFALGTAGGADALGLPVGAIAPGRRADLVALDLGDPSLWPVATLRKQIVSS
                     IQQTAVDKVMAGGRVVVDGGRPVTVDEKALRREVNAISARWSGR"
     gene            4964..>6366
                     /gene="hutH"
                     /locus_tag="VEV43_00265"
     CDS             4964..>6366
                     /gene="hutH"
                     /locus_tag="VEV43_00265"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HYP21987.1"
                     /translation="MTVHIGRPLSFADVVAVARGEGVEIDRGVADAMAPARAVVDDAV
                     ASGETVYGISTGIGDLANVRIDTGLALELQRDIVRSHATAVGEPLAREAVRAMLLLRA
                     RTFAFGVSGVRFEIVERLAALLNAGVHPVVPSQGSLGASGDLAPLAHLALPLIGEGFA
                     EVGGEVVSGAEALRRAGIEPLSLSYKEGLALVNGTEMMLALGILALDRAQSVARAADV
                     AGAMSLEACLGTDTAFRPALVALRKHPGAQVSAANLRRLLDGSPIVASHRDSDHLVQD
                     AYSLRCMPQVHGAYRDAMRYVAETLEAELGSAIDNPTVMVEEGALVSGGNFHGEALGL
                     ALDHLALCVAGFATISERRIARLVDPRLSNGLPPFLTREPGRSSGFMIAQYTAASLVS
                     ESRSACFPASADSIPSSAGQEDHVSMGATSARKAGSILTNTEHVLAIEAMAAGQGLDL
                     RAPLDPAAGTGAARAAVR"
BASE COUNT          897 a         2233 c         2310 g          926 t
ORIGIN      
        1 gaagaagtcg tagagcgcgg tctggtgcag gacgacgtcg aagcacccga gcgacggagg
       61 cgacgactcg gccaggcgct cgtacgtcgc ggccgacgcg ggttcgactc ccccggtctc
      121 gccggtgcac ctccacccga gcgcgaacgt cgtcgggtcg ccgaggtggt cgccgatcac
      181 gaggtcgccg atgcgtccga tgacgatccc gagcgcgagc ccggggacgg cgaggtcggt
      241 cgagcgccag aagcccatct tcttgcggat cgcgtacggg agagcggcca gcacggcccc
      301 cgttatcccg ccgagtagcg agatcccgcc ctcgtagatc ttgaagaccc cgaggaggtc
      361 gtcgccgccg tcggtgacct cgctgaaatg gccgaggacg tagccgagcc gggccccgac
      421 gatcgcgccg atgagcgcgt agaacagcgc gttccagatg tcgttctcgg gaggaccgtc
      481 gaacctgcgg gcccggcgca ccatcagctg ggcccccacg agatagccga tcgcgatgcc
      541 gatcccgtgg ggggagacgc tgagggcgcc gatgtcgatg gtcggggtga tcccccactc
      601 gagcgggtcg atgcccagct tgaacggcag agccaggttc acggagcgcg ccggccgaac
      661 tccacgaagc actcgtgcgt gatcggcatc ctctcggcga agaggcgctc gaccgcgtcg
      721 gcgtagacgc ggatctcgta ctgggccgcc gcggcgttgc gcagcgacag gaagttcatc
      781 agcgagcggg cgttcagcgt ccagtagaac tgcgtgtaga tcccgaacgg caggatcgcg
      841 cgggctagct ccttcgcgac gcctttctgg atcatgccct cgtactcggc gaacaggtcc
      901 ttgtagatgc gcttgaaggt gtcgatggtc tcgtcggcga gcggctcgtc gacggactcg
      961 aacgagtacg acccgggctt gccgacctgg gtgcgcaccg cctcgggggc cgggacgtag
     1021 aagtcgtcgg agagcttgtg gtaccggccc gactcctcgt tgaaggaacc cacccggtgc
     1081 ctgaaccatt cacgcgctac gaagatcggc gtccttatgt ggaagcggaa cgagttgtgc
     1141 tcgaagggcg tgccgtggcg ctcgcgcatc aggaacttga tcaggccctt gtcgcggtcg
     1201 tccatctcct cgcgcctgac cgcgaagctg accctcgccg agttcacgac cgacaggtcg
     1261 ctcgccatcg cgtcgtcgag cctcacgaag ccgtggtcga acagcagcaa ctcgtgcggc
     1321 ttgtcgccgg ggcggatctg gtcgtcgctc acaggtaacc cggggcggcc ggcggggcga
     1381 tcgtctggcc ctcggcctcg acgtagagcc ggcccccgaa ctgcgacttg atctcgtcgt
     1441 cgaagcggtc ggcggtctcg atcacggcgc cgatcgtggc ctcgagctcc tcgggggccg
     1501 cgtatccgcc gagcaggttg tggttgaacc agaccgcgcc gttgtcgacg tccagtgcga
     1561 acgccccgaa cacgaactcg aggttcctct ggaggagcca gcgggtcagc tcggccgtta
     1621 ccggcacgtc gaggttcgtg atcgcgaaca cccggacgac ggtcatgccg ttctcgagcc
     1681 aggtcggcac gacgaaagtc cgggcgctct gcatgcccac gacgtagctg ccgtggttgt
     1741 cgacgaggta gccgccgaga gaccccagct tggtctcgat catctcccgc agggcgacga
     1801 cggcctcggg ctcgcccttg ggccgcggct tggcgagctc ggactgggcg gggacctcgg
     1861 ggacggactc gatgtcgccg gcgggcgcgg gggtgggagt ggggtcgtct ggcacggagc
     1921 gcagtataag gaaggccatg cccgaacagc cggacccgat caggccgggg acgcccgctc
     1981 cgcgcggccc ccacatcagc tgcaaggggt ggccgcagga agccgccctc cgcatgctcg
     2041 ccaacaacct cgaccccgag gtggccgagc gtcccgagga cctcgtcgtc tacggggggt
     2101 cgggccgggc cgcccgcagc gtcgaggcgt tcgacgccat cgtccgggag ctgaggcggc
     2161 tcggcgacga cgagacgctg ctcgtccagt ccggcaagcc cgtcgcggtc ttccgcacgc
     2221 acgcccgggc gccgcgggtg ctgatctcga actccatgct cgtgcccgcc tgggcgacgt
     2281 gggacgagtt ccaccggctg gaagcgatgg gtctcacgat gtacggccag atgacggccg
     2341 ggtcgtggat ctacatcggg acccagggga tcctgcaggg gacctacgag acgctggccg
     2401 agctcgcggc ccggaacttc ggcggcagcc tggccggacg gatcgtcctc accgcggggc
     2461 tcgggggcat gggcggggcc cagccgctcg ccgtcacgat gaacgagggc gtcgcgctct
     2521 gcgtggaggt ggacccggcc cgcatcgagc gccggctgaa gacccgctac gtcgaccgct
     2581 cgacggacga cgtcgacgag gcgaaggcgt gggccgaggc ggcccgggac gagcggcggc
     2641 cggagtcgat cgcgcggctc ggcaacgccg cggaggtgct gcccgccctg ctcgaagccg
     2701 ggttcgcgcc cgacgtcgtc accgaccaga cgtcggcgca cgatctcctc gacggctacg
     2761 tcccggcagg gctcccgctc gacgaggcga cgcggcttag gcgcaccgac cccgacacgt
     2821 acgtgaagcg ctcgctcgag gccgtggccg cgcacgtcga ggcttggctc ggattccagg
     2881 agcgtggcgc gatctgcgtc gagtacggca acaacatccg caaccaggcc cgcaccgcgg
     2941 gcgtggagcg cgccttcgag atccccggct tcgtcgaggc ctacgtgcgt ccgctgttct
     3001 gcgagggcaa ggggccgttc cggtgggccg cgctctcggg cgatcccgcc gacatcgccg
     3061 tgaccgacga ggcgatcctc gacctcttcc cggacgacga caggctccac cgttggatcc
     3121 ggatggcgtc ggagcgcgtc gccttccagg gcctccccgc gcgcatctgc tggctcggct
     3181 acggcgaacg ggcgaaggcg ggcctcgcgt tcaacgagct cgtccgctcc ggacgtgtgt
     3241 cggcgccgat cgtcatcgga cgcgaccacc tcgacgccgg gtccgtcgcg tccccgtacc
     3301 gcgagaccga ggggatgatc gacggctccg acgcgatcgc cgactggccg atcctcaacg
     3361 cgctgctgaa caccgcggcc ggcgcgcact gggtgtcggt ccaccacggc ggcggtgtgg
     3421 gcatcggtta ctcgatccac gcgggcatgg tcgtcgtggc cgacgggacc gacgacgcgg
     3481 ccgagcggct cgagcgggtc ctcgacaccg acccgggcat gggcgtcgtg cggcacgtcg
     3541 acgcgggcta ctcacgcgcc gtcgaggtag cgagcgagcg cggcgtgcac gtcccgatgg
     3601 aggccctcgc ccgcgactag cgaccgctcc agcgggccga gatcgcgttt acctcacgcc
     3661 gcagagcctt ctcgtcaacc gtcaccgggc ggcccccgtc gacgacgacg cggcccccgg
     3721 ccatcacctt gtcgacggcc gtctgctgga tcgagctcac gatctgcttg cgcagcgtcg
     3781 cgaccggcca caaggaaggg tcgcctaggt cgagggcgac gaggtctgca cgccgcccgg
     3841 gcgctatcgc gccgaccgga aggccgaggg cgtccgctcc cccggcggtg ccgagcgcga
     3901 acgcggtctc ggcgctcacc gcggaaccgt cggtcagccg ggccttcgcc atcagcgtcg
     3961 cctggcgcat ctcttcgaac acgctctgcc ggttgttcgc gcagcccccg tcgggaccga
     4021 gggcgacgcg tatcccgcgc gagagcatct ccggcaggcg cgctatgccg tcgcccaggc
     4081 acgcgttcgc gcccgggcag tggacgaccg cggcccccgc ttccgcgagc aggtccagct
     4141 cctcgtcgtc cgcccaaacg gtgtggatcg tgacgagccg ggggccgagg agcccttccc
     4201 gtgcgagcag ccgtaccggc gtcgtcccgt agcgctcccg gacctggtcg cgctcgtagc
     4261 gggcctcggc gaggtggagg tggcagggga cgtcgagctc ttccgccagc tcgagggccg
     4321 ccgcgatcgt ctccggcgaa gcggcgtgca acgagtgcgg cgccggctgg accgagacca
     4381 tcgggtctcc cgcgtgtgca gtcgcaagag cccggcagcg atccacggag tcgccggtct
     4441 tctcgcggta gcgggcgggg gccttcgtcg gtgcgtcggc gtcgtacagg gcccgggcga
     4501 gcacgagccc gatgccggcg tcgcgcgcgg cccggatcac ctgctccgcg ttgtcgttgc
     4561 cgtcgtcgtg caggtagaag aagtcgaccg tcgtcgtgac gccggcgcgc aacgcctcgg
     4621 tgaaggcgaa cagcgcgccg gtgtagacgt cgccggcgtc gaggtcctcc gagaagggat
     4681 acaggacgtc gtcgcgccac gagtcgaacg tccggtcgtc ggcccatccc ttgaggaggt
     4741 tctggaacgc atggccgtgc gcgttcacgg tgccggggac gatcgctacg tctccccagt
     4801 cgacggcctc gacggagtcg cgtccgacga cgtcggcgat cgtgtctccc tcgaccacga
     4861 cggcgacgtc gccggtgaac ccgtcctcgg tgaggacgag cgcgcccgtg atcgccttcg
     4921 tcgtcatcgt cgaacccccc gaccgagctt taggctgccg cccgtgaccg tacacattgg
     4981 gaggccgctg agcttcgccg acgtagtcgc cgtcgcgcgc ggagaagggg tcgagatcga
     5041 ccgcggcgtc gccgacgcga tggcgccggc ccgcgccgtg gtcgacgacg cggtcgcgtc
     5101 cggcgagacc gtctacggga tctcgaccgg gatcggcgac ctcgccaacg tgcgtatcga
     5161 caccgggctc gcgctcgagc tccagcgcga catcgtgcgc tcccacgcga cggcggtcgg
     5221 cgagccgctg gcgcgggagg ccgtacgggc gatgctgctg ctgcgggcgc ggacgttcgc
     5281 gttcggtgtc tcgggcgtcc gcttcgagat cgtcgagcgg ctcgccgcgc tgctgaacgc
     5341 gggggtccat ccggtcgtgc cgtcgcaggg gtccctcggt gcgtccggcg acctcgcccc
     5401 gctcgctcat ctcgcgctgc cgctgatcgg cgaggggttc gccgaggtcg gcggcgaggt
     5461 ggtctccgga gccgaggcgt tgcgccgcgc ggggatcgag ccgctgagcc tctcctacaa
     5521 ggaaggcctc gccctcgtga acgggaccga gatgatgctc gcgctcggca tcctcgccct
     5581 cgaccgtgcg cagagcgtcg cgcgcgcggc ggacgtcgcc ggcgcgatgt cgctcgaggc
     5641 gtgcctcggg accgacaccg ccttccggcc cgccctagtc gcgctgcgca agcaccccgg
     5701 cgcgcaggtc tccgccgcga acctgcgccg gctcctcgac gggagcccga tcgtcgcgtc
     5761 gcaccgggac tcggaccacc tcgtgcagga cgcgtactcg ctgcgctgca tgccgcaggt
     5821 ccacggtgcc taccgcgacg cgatgcgtta cgtggccgag accctcgagg ccgagctcgg
     5881 gtccgcgatc gacaacccga cggtgatggt cgaggaaggg gcgctggtgt cgggcgggaa
     5941 cttccacggc gaggcgctgg ggctggcgct cgaccacctc gcgctgtgcg tggcgggctt
     6001 cgccacgatc tccgaacggc ggatcgcacg cctcgtcgac ccgcggctga gcaacgggct
     6061 cccgccgttc ctcacgcggg agccgggccg gtcgtcgggg ttcatgatcg cccagtacac
     6121 cgcggcgtcg ctggtgtcgg agagccgctc ggcgtgcttc ccggcatccg ccgactcgat
     6181 cccgtcgtcg gccgggcagg aggaccacgt cagcatgggc gcgacctcgg cccgcaaggc
     6241 gggctcgatc ctgacgaaca ccgagcacgt gctggcgatc gaggcgatgg ccgcggggca
     6301 ggggctggac ctgcgggcac cgctcgatcc ggccgcgggg acgggggccg cgcgtgcggc
     6361 cgtccg
//
LOCUS       DASQDO010000013         6697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_858765, whole genome shotgun sequence.
ACCESSION   DASQDO010000013 DASQDO010000000
VERSION     DASQDO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858765"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VEV43_00270"
     CDS             <1..417
                     /locus_tag="VEV43_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21988.1"
                     /translation="AALVPGRAMASTTTCQPVNRPAVTVGVAGEPVATTPGVFVEVCV
                     TVPSPEVTGTPAVRQEDYNCCSLALNRHFAVFLDSGVSSPPSTVTVSVTYSVDGVGDT
                     ETVSVPVGGTSGSSTCLFFRGSPANNPGGCLVFLEE"
     gene            701..2071
                     /gene="dnaB"
                     /locus_tag="VEV43_00275"
     CDS             701..2071
                     /gene="dnaB"
                     /locus_tag="VEV43_00275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HYP21989.1"
                     /translation="MAQRAPGNGISTLDRPRPNRIPPHNIDAEQSVLGAMLESKEAIA
                     NVVESLKPDDFYKPAHGEIYQTILDLYGRGEAADAITVADELKRRGSLEQIGGKPYIH
                     GLLEAYPTASSAAHYARIVEEHAQLRRLISAGVEVQEIGFSIPEDVAAATDQAEEVIY
                     QVADRRLRDTVHPIRDLLIEGMENIEALCERGESVTGIASGFPDLDDWTTGFQASNLV
                     IVAARPSMGKSALMGDFALNAAVRQQKPVVIFSLEMGRQEMVQRFLASEGKVDSQRIR
                     KGSLQEQDWTRLSAALGRLAEAPIVIDDSANITLMEMRAKCRRLKARHGLGMIIIDYL
                     QLMQGPRRSENRQQEVSEISRSLKILAKELQVPVVCASQLNRMVDSRSDKRPMLGDLR
                     ESGAIEQDADIVMFLYRDEVYDPNSEARGEAELIVAKHRNGPTGTVRLAFMNQYTKFA
                     SIARVP"
     gene            2183..2761
                     /locus_tag="VEV43_00280"
     CDS             2183..2761
                     /locus_tag="VEV43_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYP21990.1"
                     /translation="MTVLSQHTSDVNTARAFAGLKERFPAWREVVDAPAKDVADAIRS
                     GGLADQKAPRIQSILREIEAREGDLSLARLAALDDDEVEDYLCSLPGVGPKTAACVLV
                     FSMGRQAFPIDTHVHRVTARLGWIPQAASADKAHALLKKTVPPELRYELHVQLITHGR
                     TICKARMPRCTDCVLFDLCDAGPHLLAAGDAR"
     gene            2831..3532
                     /locus_tag="VEV43_00285"
     CDS             2831..3532
                     /locus_tag="VEV43_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21991.1"
                     /translation="MRSSKRVLGLAVAAAAVAGLTVLPSAAQAQSCTPVPLSTTGITI
                     EGAGREVRVPSVSGVAVCYDVPSTTGIPWVETEPGGGFSVLITGSSGSDGSVSLRYTA
                     DGSSEEIEVPLPGSGGNGETCLVGVGFPARDDCAFKISPDNDPVPTLPPVSPPPVPTA
                     PPVPPRPPLPTAPPVPTVPPIPDDVCGNPRTCLSDLPYIIDRAIAQVIARVYDILNDI
                     VIVNCGPILQLLGLC"
     gene            3581..4300
                     /locus_tag="VEV43_00290"
     CDS             3581..4300
                     /locus_tag="VEV43_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21992.1"
                     /translation="MKKVLAQLVAVAAVGAVIAIPGPAHAESCVTVPVGSSGFTLEAA
                     GEEVRIPAIASASVCYQIPGTPGIPWVKTEYGGASVVVTGGSATGGHVALRYVADGST
                     RSVTAPLPATGGGGRETCLFGVGSPYARPDCAVKLHADQLVPSPLPTAPPIPTTGPLP
                     TRPPLPTLPPVPSAPPTPEPNPLCPTASQCIPYGGELQGLVDGFYEFLREDVAPAVRE
                     LQDRIKEIVDAISIGCTPVTC"
     gene            complement(4350..4423)
                     /locus_tag="VEV43_00295"
     tRNA            complement(4350..4423)
                     /locus_tag="VEV43_00295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4389..4391),aa:Gly,seq:ccc)
     gene            4622..4873
                     /locus_tag="VEV43_00300"
     CDS             4622..4873
                     /locus_tag="VEV43_00300"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HYP21993.1"
                     /translation="MESWVGPDPVPVYTTLTEVEAHLVRSALVAADIPSVVWSSGHGG
                     YYGHAIGLAPFPHRVMVHRDDEAEARDLLAARFPDGTGE"
     gene            4885..5454
                     /gene="dcd"
                     /locus_tag="VEV43_00305"
     CDS             4885..5454
                     /gene="dcd"
                     /locus_tag="VEV43_00305"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HYP21994.1"
                     /translation="MILSDRDIRKAIGDGRIGIDPFDEADVQPSSVDLHVDRYFRTFH
                     NHRHPYIDVKQPMENLTELVEVDGDEAFILHPGEFVLGSTAEYVKLPDDLVARLEGKS
                     SLGRLGLLIHSTAGYVDPGFEGHLTLELSNVANLPITIYPGMKIGQISFFQLTSPADH
                     PYGTSKVGSKYQGQRGPTPSRYFENFRRG"
     gene            5465..5998
                     /locus_tag="VEV43_00310"
     CDS             5465..5998
                     /locus_tag="VEV43_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYP21995.1"
                     /translation="MEERIVAPDPVEDPQGYQQALLDLLGDEEALDVLASTPEKIEDL
                     CAELDVAILQKEPAPGEWSAEGVVAHLLDAEVVYSFRWRMTLAQSGTAFPGYQQDLWT
                     DLPHPSFPEMLAAFSALRRLNVWLAEETPSDQWDDKVGVHSERGPETFDVSLKVVAGH
                     DLAHLKQLEQTLAAVQT"
     gene            6094..>6697
                     /locus_tag="VEV43_00315"
     CDS             6094..>6697
                     /locus_tag="VEV43_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYP21996.1"
                     /translation="MFDRLAEFVVRRRLWIVVATGIFFVAAGAIGGGVSERLSSGGFE
                     DPNAESTRAEEVLRREFDTQTPNVILLVTAENGDVDDPAVAEQGLALTEELAAQEGVE
                     QAASYWSLPAPPLKSTAGDQALVIGVVEGDDDAIRERMEELSPHFTRSGDGIDVEVGG
                     FAEIYRQMATTIEEDLVRAETIAIPIPMVLLVVVVGSVVAA"
BASE COUNT         1059 a         2419 c         2229 g          990 t
ORIGIN      
        1 gcagcgctgg taccaggcag agccatggcg tccacgacca cgtgtcagcc ggtgaaccgt
       61 cctgcggtca ccgtcggcgt ggcaggcgag ccggtcgcca ccacccccgg cgtcttcgtc
      121 gaggtgtgcg tcacggtccc gtcccccgag gtcaccggga ctccggcggt tcggcaggag
      181 gactacaact gctgctcgct ggccttgaac cggcactttg ccgtcttcct ggactcgggg
      241 gtgtcgtctc cgccgtccac cgtcaccgtc agcgtgacgt actcggtcga cggtgtggga
      301 gacaccgaga ccgtcagcgt cccggtgggt ggcacgtcgg gctcgagcac gtgtctgttc
      361 ttccggggga gccccgcgaa caaccccggc ggctgcctcg tcttcctcga ggagtagcgg
      421 gcgcagaggt ctcggggggc cttcgggccc ccctttcttt ttcgcgcttc cacaggttgt
      481 gggcccgggg ttgcgaggcg ggcttgagct cccctcgttc tccacgcctt ccacagccga
      541 cttacgttcc acaggccacg gccgctagct ttctcccggg tcctcgcggc gcccggcact
      601 tccccggccc aatccgcgaa atcccagcaa ccctcgcgga taatcgaaca cccgttcgcc
      661 ccaccgacgg gatgcaccga ccaaacggcc aggagggacc ttggcgcagc gcgcaccagg
      721 caacggcata tcgacgctcg accggccccg gcccaaccgg atcccgccgc acaacatcga
      781 cgcggagcaa tcggtcctcg gcgccatgct cgagtcgaag gaggcgatcg ccaacgtcgt
      841 cgagtccctc aagcccgacg acttctacaa gcccgcccac ggcgagatct accagacgat
      901 cctcgacctc tacgggcggg gcgaggccgc cgacgccatc acggtcgccg acgagctcaa
      961 gcggcggggc tccctcgagc agatcggcgg caagccctac atccacggcc tcctcgaggc
     1021 gtatccgacg gcgtcgagcg cggcccacta cgcgcggatc gtcgaggagc acgcccagct
     1081 ccgccggctg atctccgcgg gcgtcgaggt ccaggagatc ggtttctcga tccccgaaga
     1141 cgtcgccgcc gcgaccgacc aggccgaaga ggtcatctac caggtcgccg accgccgcct
     1201 gcgtgacacc gtccacccga tcagggacct gctgatcgag ggcatggaga acatcgaggc
     1261 gctgtgcgaa cggggcgaga gcgtcaccgg catcgcgtcg ggcttccccg acctcgacga
     1321 ctggaccacc ggcttccagg cctcgaacct cgtcatcgtc gcggcccggc cctcgatggg
     1381 taagtccgcg ctgatgggcg acttcgcgct gaacgccgcg gtgcgccagc agaagcccgt
     1441 cgtgatcttc agcctcgaga tgggtcgcca ggagatggtc cagcgcttcc tcgcgtccga
     1501 gggcaaggtc gactcgcagc gcatccgcaa gggatcgctc caggagcagg actggacgag
     1561 gctgtccgcc gcgctcggcc gtctcgccga ggccccgatc gtcatcgacg actcggcgaa
     1621 catcacgctg atggagatgc gcgcgaagtg ccggcgcctc aaggcccggc acgggctcgg
     1681 catgatcatc atcgactacc tccagctgat gcaggggcct cggcgctcgg agaaccgcca
     1741 gcaggaggta tccgagatct cgcggtctct gaagatcctc gcgaaggagc tgcaggtccc
     1801 ggtcgtgtgc gcgtcgcagc tgaaccggat ggtcgactcg cgcagcgaca agcggccgat
     1861 gctcggcgac ctgcgcgagt cgggcgcgat cgagcaggac gccgacatcg tgatgttcct
     1921 gtaccgggac gaggtgtacg acccgaactc ggaggcgcgc ggcgaggccg agctcatcgt
     1981 cgcgaagcac cgcaacggcc cgacgggcac ggtgcgcctc gcgttcatga accagtacac
     2041 gaagttcgcc tcgatcgccc gggtgccgta gctggagcct cggcccgacc gccgccgcat
     2101 ccgggccgtc caccggcggc tcctgaagca acagggtccg ttcacaccga agccgcggct
     2161 ggacccgctc gacgagctcg tgatgaccgt gctctcgcaa cacacgtcgg acgtgaacac
     2221 cgcgcgcgcg ttcgcggggc tcaaggaacg cttcccggcg tggcgcgagg tcgtcgacgc
     2281 gccggcgaag gacgtcgcgg acgcgatccg ctccggcggg ctcgcggacc agaaggcgcc
     2341 gcgcatccag tcgatcctgc gcgagatcga ggcgcgtgaa ggcgacctgt cgctggcgcg
     2401 gctcgccgcc ctcgacgacg acgaggtcga ggactacctc tgctcgctgc cgggcgtcgg
     2461 cccgaagacc gcggcctgcg tgctcgtttt ctcgatgggc cgccaggcgt tcccgatcga
     2521 cacccacgtg catcgggtga cggcccgcct cggctggatc cctcaggccg cgtctgccga
     2581 caaggcccac gcgctgctga agaagaccgt cccgcccgag ctccgctacg agctacacgt
     2641 ccagctgatc acccacggcc gcacgatctg caaggcccgc atgccgcgct gcaccgactg
     2701 cgtgctgttc gacctctgcg acgcggggcc gcacctcctc gccgccggcg acgcccgcta
     2761 ggaggagacc aagcaaccgc gtcgaagacg tgcgggtcaa gtggcgggca acaatcagga
     2821 ggtggggtag ttgaggtcgt ccaagcgcgt cctcgggctg gcggtggctg ctgcggcagt
     2881 cgcgggcctg acggtgctgc cgagcgcagc gcaggcacag tcttgtacgc cggtaccgct
     2941 cagcacgacc ggcataacga tcgagggggc cggccgcgag gtccgcgtcc cgtccgtctc
     3001 cggcgtcgcg gtctgctacg acgtcccgag caccacgggg atcccgtggg tcgagaccga
     3061 gcccggcggc ggcttctccg tcctcatcac cgggagctcc ggcagtgacg ggtccgtcag
     3121 cctcaggtac acggcggacg gctcgtcgga agagatcgag gtcccgctcc ccggctcggg
     3181 cggcaacggc gagacctgcc tggtgggcgt cggcttcccg gcgcgagacg actgcgcctt
     3241 caagatctcg ccggacaacg acccggtccc gaccctgccc ccggtctcgc ccccgccggt
     3301 cccgacggca ccgccggtcc cgccgcggcc cccgctcccg acggctccgc cggtcccgac
     3361 ggtcccgccc atcccggacg acgtgtgcgg caacccgagg acctgcctga gcgacctccc
     3421 gtacatcatc gaccgggcga tcgcccaggt gatcgcgagg gtgtacgaca tcctcaacga
     3481 catcgtcatc gtcaactgcg gccccatcct ccagctgctg ggtctctgct aggcgagctg
     3541 ccgacaggtc attctggcga acgacgaagt gggaggttcc atgaagaaag tgctcgcgca
     3601 gctggtcgcg gtagcggccg tcggcgcagt catcgcaatc cccggccccg ctcacgcgga
     3661 gagctgcgtc accgtcccgg tcgggtcgtc cgggttcacg ctggaagccg ccggggaaga
     3721 agtacgcatc ccggcgatcg ccagcgcgtc cgtctgctac cagatcccag ggacgccggg
     3781 catcccgtgg gtgaaaaccg agtacggcgg cgcgtcggtg gtcgtcaccg gcggctccgc
     3841 gaccgggggc cacgtggctc ttcgttacgt cgcggacggg tcgaccaggt cggtgaccgc
     3901 gccgctcccg gccaccggtg gcggcggaag ggagacctgc ctcttcggcg tcggctcgcc
     3961 gtacgcgcgc ccggactgtg cggtgaagct ccacgcggac cagctcgtcc cgagcccgct
     4021 gccgaccgcg ccgcccatcc cgacgacggg cccgcttccg acgcggcccc cgctcccgac
     4081 cttgccgccg gtccctagcg ctcctccgac accggagccg aacccgctgt gcccgacggc
     4141 gagccagtgc atcccctacg gaggcgagct ccaggggctc gtcgacgggt tctacgagtt
     4201 cttgagggag gacgttgccc ccgcggtcag ggagctccag gacaggatca aggagattgt
     4261 cgacgcgatc tccatcggct gcacgcccgt cacctgctag cacggtgtga cgaacgaggg
     4321 cccggcttcg gccgggccct ttcgcgtggt ggagcgggtg aagggaatcg aaccctcgtc
     4381 actagcttgg gaagctagag ctctaccatt gagctacacc cgcggcgatc acggatcata
     4441 gcgccgcggc tcagggggga ggcgggcgat cgacccgaac gaaggcagat accggtactg
     4501 cccgcgctgc cggaccgagt accggcccgg cttcgagatc tgcaccgatt gcggcgtcga
     4561 cctcgtcgac gagctccccg gcgcgggaga ggcgtcgcaa ccggaacccg atcgcgaggc
     4621 tctggagtcc tgggtcggcc ccgaccccgt ccccgtctac acgaccctca ccgaggtcga
     4681 ggcgcacctc gtccggagcg cgctcgtggc cgcggacatc ccgtccgtcg tctggtcgag
     4741 cggccacggc ggctactacg gccacgcgat cgggctcgcc ccgttcccgc accgcgtgat
     4801 ggtgcaccga gacgacgagg ccgaggcgcg cgatctgctc gcggctcggt tcccggacgg
     4861 aactggcgag tagactcccg cgccgtgatc ctgtccgacc gagacatccg caaggcgatc
     4921 ggcgacggcc gcatcgggat cgatcccttc gacgaggccg acgtccagcc ctcgtcggtc
     4981 gacctccacg tcgaccgcta cttcaggacg ttccacaacc accggcaccc gtacatcgac
     5041 gtgaagcagc cgatggagaa cctcaccgag ctcgtcgagg tcgacggcga cgaggccttc
     5101 atcctccacc ccggcgagtt cgtgctcggc tccaccgccg agtacgtgaa gctgcccgac
     5161 gacctcgtcg cccggctcga gggaaagagc tctctcggcc gtctcggcct gctgatccat
     5221 tcgaccgccg gctacgtcga cccgggcttc gagggccacc tgacgctcga gctcagcaac
     5281 gtcgccaacc tccccatcac gatctacccc ggcatgaaga tcggccagat cagcttcttc
     5341 cagctgactt cgcccgccga ccacccctac ggaaccagca aggtcggctc caagtaccag
     5401 ggccagcggg gcccgacgcc ctcccgctac ttcgagaact tccgacgcgg ctaggaggcc
     5461 cccaatggaa gaacgcatcg tcgccccgga cccggtcgag gacccgcagg gctaccagca
     5521 ggcgctgctc gacctgctcg gcgacgagga ggcgctcgac gtcctggcgt ccacgcccga
     5581 gaagatcgag gacctgtgcg cggagctcga cgtcgcgatc ctgcaaaagg agccggcgcc
     5641 cggcgaatgg tcggcggagg gcgtcgtcgc ccacctcctg gacgccgagg tcgtctactc
     5701 gttccggtgg cggatgacgc tcgcgcagtc cgggaccgcc ttccccggct accaacagga
     5761 cctgtggacg gaccttcccc atccgtcgtt cccggagatg ctcgccgcgt tctcggccct
     5821 gcgccgcctg aacgtatggc tcgccgagga gacaccgtcg gaccagtggg acgacaaggt
     5881 gggcgtgcac tcggaacggg ggccggagac cttcgacgtc tcgctgaagg tggtagcggg
     5941 gcacgacctg gcgcacctga agcagctgga gcagaccctg gccgccgtcc agacctaacg
     6001 gcgttcacca acgatcgcgg gacccccttt acatcgttag cttatcggcg ttaggttaac
     6061 gatgttcagc aaggcacgag aggggagttt cccgtgttcg atcggctcgc ggagttcgtc
     6121 gtcaggcggc ggttgtggat cgtcgtggcc accggcatct tcttcgtcgc cgccggggcg
     6181 atcggcgggg gcgtctccga gcggctctcg tcgggcgggt tcgaggaccc gaacgcggaa
     6241 tcgacccggg ccgaagaggt cctgaggcgc gagttcgaca cccagactcc taacgtgatc
     6301 ctgctcgtca ccgccgagaa cggtgacgtc gacgatcccg ccgtcgccga gcaggggctt
     6361 gcgctgaccg aggagctcgc ggcccaggaa ggcgtcgagc aggcggcctc ctactggtcg
     6421 ctcccggccc ccccgctgaa gagcaccgcg ggcgaccagg ccctcgtgat cggcgtcgtc
     6481 gaaggcgacg acgacgctat ccgggagcgc atggaggagc tgtcgccgca cttcacccgt
     6541 agtggcgacg gcatcgacgt cgaggtgggt gggttcgccg agatctaccg gcagatggcc
     6601 acgacgatcg aagaggacct cgttcgcgcc gagacgatcg ccatcccgat cccgatggtg
     6661 ctgctcgtcg tcgtcgtcgg cagcgtcgtg gccgccg
//
LOCUS       DASQDO010000014         7854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_430580, whole genome shotgun sequence.
ACCESSION   DASQDO010000014 DASQDO010000000
VERSION     DASQDO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430580"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VEV43_00320"
     CDS             <1..706
                     /locus_tag="VEV43_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYP21997.1"
                     /translation="ISKVFTSTHFVDYITAAAPGLKDILMLGKIWYLEQGRTNSRGHG
                     DFDTIVVDAPAAGHMLTFLSAPMGLADAVRIGPVKRQSDWLVEMLRDPKRTRVHLVTL
                     PEEMPVSETLETAEALRTRIGIDAGAVFANAVYSELFDKDEKQTLADIGGAKPMAALK
                     KIAAEAGLTLDSEDVAALIGYARFLTARQGIQGVHLKKLRRGVAEPVVELPFLFSAGL
                     ALPDIENLADVIEAAA"
     gene            703..1869
                     /locus_tag="VEV43_00325"
     CDS             703..1869
                     /locus_tag="VEV43_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HYP21998.1"
                     /translation="MNPISGILADKEVVVCAGAGGVGKTTTAAAIALKGALEGKRTAV
                     LTIDPARRLASSLGLQELSNEPTKVSTRKFSAVGVKPKGELYALMLDTKTTFDRVVNR
                     YAPTPEQAERIIANRFYRNISSTLSGTQEYMAMEKLYELHAEGGFDVVVIDTPPTRNA
                     LDFLDAPQRMNEFFDSRVLRWFLLPYMKAGGGIMRVANVAATTFLKIVKRIVGTEVLE
                     DTAEFFTNLDGMYDGFKERAREVAELLQSDATSFVVVTSPSEDSVAEATYFAERLNDS
                     GLPFGALVVNRVHPKIGDGIDVKQRQLHKLEAGDEDARLLAKLLENEEAFMRVVHLEE
                     QNLAELARRVPRHKWVRVPYLEQEAVDFSGLLAIAEQLFGEPATPGPRAERGGA"
     gene            1977..2366
                     /locus_tag="VEV43_00330"
     CDS             1977..2366
                     /locus_tag="VEV43_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP21999.1"
                     /translation="MRKARFAAAALAVVSVMSFAPAANAATCQPIVDSTLGVHLSLCG
                     EVDTDPSSLPSLRYESLGDPTSFAIYLDVPEEFGGQGDVWLRYQLGGPTQEYYLPPNG
                     EWDSSICVFYTAPAAAAPGTCIFGISR"
     gene            2438..2920
                     /gene="sdhC"
                     /locus_tag="VEV43_00335"
     CDS             2438..2920
                     /gene="sdhC"
                     /locus_tag="VEV43_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HYP22000.1"
                     /translation="MLAAKLIEGFVFLLTAASALFAVVVLADLFRIRSKDNGPAYRGG
                     VGQWSWAAHRITGVFVLAFLFGHIVDTFAIGFGPELYDETISLYQQWWFKPFEVGLIA
                     AVVYHAFNGLRIILFDFWPQLALYQRPIAYVEFALCLVAFAPAAFFMLRSAWEGSPFS
                     "
     gene            2920..3783
                     /locus_tag="VEV43_00340"
     CDS             2920..3783
                     /locus_tag="VEV43_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22001.1"
                     /translation="MAALDTDRLATTKEVVARAVSGRALKKGSGGKATLRIEREASVR
                     RIVQAYWTPFEVAVPGECISLNLEVAEPTVHWGEAYLTPDYRNWVLFFAERPDGEIQL
                     VHYADFATPEEAVQAFFDKATWYTLIPTDNVWEGVEYSVAESLDWGDVETAVEAGSVG
                     PGVQESLKKSTIIWLRWRDGDGHDRTMPVWFLYDNKAQKIYVISGERQQTIPGAERLR
                     EVDVIFRWKGKNARIGEVPARVRVMPHGSEWDTVAEKVAEKRLNIPGLPEDTARRWRD
                     ECVLLELTLLS"
     gene            complement(3796..4068)
                     /locus_tag="VEV43_00345"
     CDS             complement(3796..4068)
                     /locus_tag="VEV43_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYP22002.1"
                     /translation="MSSTQLVEQDWRAMGSCSKTDPDLWFAVGAMEHKQAKRICRECP
                     VQGQCLAYAMDAPVDHGIWGGLTERERRRFRRQAGAKDWRALLQSN"
     gene            4307..5122
                     /locus_tag="VEV43_00350"
     CDS             4307..5122
                     /locus_tag="VEV43_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HYP22003.1"
                     /translation="MRHLRYLLVALAVGALSAGSLPSAAAQAAPSYQSSDPAKDEMMD
                     HPPTSVTVTFDQPLDQSSWMNVLDECGKEIDAGAATVDLNEMTVDIGKSPSGTYEVVY
                     KAVGVAGVTGESASSFEFMVHHGKPCGAKKKDHHHDPDKKKPDHHDHGDDDDGPGHDD
                     HEDMDMGSGSGSSDHSDHPGMSGSSGSSGHSDHSTASTGHEGAGHGGRHGNHDPGKPG
                     GQATGATDPPVLATGDGAVPIGADGQAVLVGLGLALAEGVLGGWLLRVSGNLA"
     gene            complement(5119..5514)
                     /locus_tag="VEV43_00355"
     CDS             complement(5119..5514)
                     /locus_tag="VEV43_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HYP22004.1"
                     /translation="MIVVDTSIWIDVLNDVESPGASRCVELLQGGAPVALTDVVFTEI
                     LQGLGDEREARLVENHLKAFPILRLEALGDFALAAELYRTARRAGVTIRKTLDCLIAA
                     PCVRDDVPLLHADEDFNRLASCTNLKVLG"
     gene            complement(5511..5708)
                     /locus_tag="VEV43_00360"
     CDS             complement(5511..5708)
                     /locus_tag="VEV43_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYP22005.1"
                     /translation="MRTNIDIDDDVLREVQRLTGVKTKRAAVDFALREVVAQHRRLGL
                     LGLRGKVRWEGNLDESRSGRT"
     gene            complement(5748..6767)
                     /locus_tag="VEV43_00365"
     CDS             complement(5748..6767)
                     /locus_tag="VEV43_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HYP22006.1"
                     /translation="MALTLTRFAGFAANALVLGTLAVVVLVLRPTFASLDPEAWSRGR
                     TRLAERLEGLVHSALIGAAAAAALALVLQTVLTAELLGGDVTSDSFFAVLETSFGPWY
                     AARIPIVAGLLVLLTGKVRQWALAQRGVDSSVAGPAWWGAWAVLGVVLLATSSFSGHA
                     AVATPRIVSLGTDVVHLVAGSIWFAGIVLLSVALPDSWVGKDDAARLEVLSPAVRHFS
                     HVAIVAIGIVLVTGMVGSILHVGHPGDLVETPYGRTLTIKILFFGLILALGAFNHFVI
                     RKRFETALEKSSSAGSATRTFRKAIAAELAIGLILMGLTGWLTGQSRTREVVVEPSRV
                     TAEQP"
     gene            6813..6935
                     /locus_tag="VEV43_00370"
     CDS             6813..6935
                     /locus_tag="VEV43_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22007.1"
                     /translation="MTSAQLVLVSVTSALIAVFAMLVVATIRRARTRDEESKKD"
     gene            complement(6932..7285)
                     /locus_tag="VEV43_00375"
     CDS             complement(6932..7285)
                     /locus_tag="VEV43_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYP22008.1"
                     /translation="MSQQKIEIDVRNQGDYQVLRPEGDLDVYTVGSLRDAIGTMIEDG
                     TPHVVVDLDGVPFMDSSGLGALMGGVRRLREAGGDLAIACTREQHLKLFTITGFGEGV
                     SIAPTVEEAAAGFRS"
     gene            complement(7300..>7854)
                     /locus_tag="VEV43_00380"
     CDS             complement(7300..>7854)
                     /locus_tag="VEV43_00380"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYP22009.1"
                     /translation="KRVIEQANLALLEATETGAFTTCVYATVDIVTGEVRWLNMGHPA
                     PFLLHAPDPARDGGSPGNFVEAEGPRNRPLGWDENPGLNQTTATLAPGDRLILFTDGF
                     LEAKSPEGEVFGEHRLEASLLELASLPSDDLAGHVVEDVERFAAGQLDDDLTMLVIEF
                     EGAAAPGSDDLPPATRVGAAPEAE"
BASE COUNT         1280 a         2710 c         2676 g         1188 t
ORIGIN      
        1 gatcagcaag gtcttcacga gcacgcactt cgtcgactac atcaccgccg cggcccccgg
       61 cctcaaagac atcctgatgc tcggcaagat ctggtacctc gagcagggcc gcaccaacag
      121 ccgagggcac ggggacttcg acacgatcgt cgtcgacgcg ccggccgccg ggcacatgct
      181 gacgttcctc tccgcgccga tgggcctcgc cgacgcggtt cggatcgggc cggtgaagcg
      241 gcagtccgac tggctcgtcg agatgctccg cgacccgaag cggacccgcg tccacctcgt
      301 gacgctgccc gaggagatgc cggtgtccga gacgctcgag accgcggagg ctctgcgcac
      361 gaggatcggg atcgacgcgg gggccgtgtt cgctaacgcg gtgtactccg agctcttcga
      421 caaggacgag aagcagacgc tcgcggacat cggtggcgcg aagccgatgg ccgcgctgaa
      481 gaagatcgcg gccgaggcgg gcctgacgct cgactccgag gacgtggcgg cgttgatcgg
      541 gtacgcgcgc tttctgaccg cgcggcaggg catccaggga gtccacctca agaagctccg
      601 gcgcggggtg gccgagccgg tcgtcgagct cccgttcctg ttctcggccg ggctcgcgct
      661 ccccgacata gagaacctgg ccgacgtcat agaggccgcc gcgtgaaccc gatcagcggg
      721 atcctcgccg acaaggaggt cgtcgtctgc gcgggcgccg gcggcgtcgg caagacgacc
      781 actgccgcgg cgatcgcgct caagggagcg ctcgaaggca agaggacggc ggtgctcacg
      841 atcgacccgg cgcggaggct cgcgtcgtcg ctcggtctcc aagagctgtc gaacgagccg
      901 acgaaggtca gcacgcgcaa gttctcggca gtcggcgtga agccgaaggg cgagctgtac
      961 gcgctgatgc tcgacacgaa gacgacgttc gaccgcgtcg tcaaccgcta cgcgccgact
     1021 cccgagcagg cggagcgcat catcgccaac cgcttctacc ggaacatctc gtcgaccttg
     1081 tcggggacgc aggagtacat ggcgatggag aagctctacg agctccacgc cgagggcggc
     1141 ttcgacgtcg tcgtcatcga cacgccgccg acgcgcaacg ccctcgactt cctcgacgcg
     1201 ccgcagcgga tgaacgagtt cttcgacagc cgcgtgctgc gctggttcct gctcccgtac
     1261 atgaaggccg gcggcggcat catgcgcgtc gcgaacgtcg ccgcgacgac gttcctcaag
     1321 atcgtcaagc gcatcgtcgg caccgaggtc ctcgaggaca ccgcggagtt cttcaccaac
     1381 ctcgacggga tgtacgacgg gttcaaggag cgggcacgcg aggtcgccga gctgctccag
     1441 tccgacgcga cgtcgttcgt cgtcgtcacg tcgccctcgg aggactcggt ggcagaggcg
     1501 acctacttcg ccgagcgtct caacgactcc gggctcccgt tcggcgcgct cgtcgtgaac
     1561 cgcgtgcacc cgaagatcgg cgacgggatc gacgtcaagc aacgccagct ccacaagctc
     1621 gaggccggcg acgaggatgc ccggctgctg gcgaagctcc tcgagaacga ggaggcgttc
     1681 atgcgtgtcg tccacctcga ggaacagaac ctggcggagc tggcccggcg ggtcccgagg
     1741 cacaagtggg tgcgggtgcc gtacctcgag caggaagccg tcgacttctc gggcctgctg
     1801 gcgatagcgg agcagctgtt cggcgagccg gcgacacccg gcccccgggc cgagcgcggc
     1861 ggggcctagg cactgacggc ggaggtcagg tcttcgccat cctcggcaac gagcaagggg
     1921 agcaggccgg gctcgtcgaa tgcgcggggg ttgtcgctag acggacggag gtagtcatga
     1981 ggaaggcccg cttcgccgcg gccgcgctcg cggtcgtgtc tgtcatgtcg ttcgcaccgg
     2041 cagcgaacgc cgctacgtgt cagccgatcg tcgactcgac gctgggcgtc catctctcgc
     2101 tctgcggcga ggtcgacacg gatcccagct cgctcccgtc gctccgctac gagagcctcg
     2161 gcgacccgac gtcgtttgcg atctacctcg acgtccccga ggagttcggc ggccaggggg
     2221 acgtgtggct caggtaccag ctcgggggcc cgacccagga gtactacctg ccgccgaacg
     2281 gcgagtggga ctcgtcgatc tgcgtgttct acacggcccc cgccgcggcg gcgcccggga
     2341 cttgcatctt cggcatcagc cggtagccac aggccccttg gatacgcccc cggcccccga
     2401 cgccgggggc gtatcgcgcg ttgggtagga tcgggtcgtg ctcgccgcga agctcatcga
     2461 agggttcgtc ttcctcttga ccgcagcgtc ggcgctgttc gccgtcgtcg tgctggccga
     2521 cctgttccgg atccgctcga aggacaacgg cccggcctac cgcggcggcg tcggccagtg
     2581 gtcgtgggcc gctcaccgga tcacgggcgt cttcgtcctc gccttcctgt tcggccacat
     2641 cgtcgacacg ttcgcgatcg ggttcggccc cgagctctac gacgagacga tcagcctcta
     2701 ccagcagtgg tggttcaagc cgttcgaggt cgggctgatc gcggccgtcg tgtaccacgc
     2761 gttcaacggg ctccgcatca tcctgttcga cttctggccg cagctcgcgc tctaccagcg
     2821 gccgatcgcg tacgtcgagt tcgccctctg cctggtcgcc ttcgcgcccg ccgcgttctt
     2881 catgctgcgg tcggcgtggg aagggtcacc gttctcctga tggcagccct ggacaccgac
     2941 agactcgcca ccacgaaaga ggtcgtcgcg cgcgccgtct ccgggcgcgc cctgaagaag
     3001 gggtcgggcg gcaaggccac gctgcggatc gagcgcgagg cgagcgtccg gcggatcgtc
     3061 caggcgtact ggaccccctt cgaggtcgcc gtccccggcg agtgcatcag cctgaacctc
     3121 gaggtcgccg agcccaccgt ccactggggc gaggcgtatc tcactcccga ttaccgcaac
     3181 tgggtcctgt tcttcgccga gcgtcccgac ggcgagatcc agctcgtcca ctacgccgac
     3241 ttcgcgacgc ccgaggaggc cgtgcaggcg ttcttcgaca aggccacctg gtacacgctg
     3301 atcccgaccg acaacgtttg ggagggcgtc gagtactcgg tggccgagtc gctcgactgg
     3361 ggcgacgtcg agaccgcggt ggaggcgggc tcggtaggcc ccggcgtcca ggagtcgctc
     3421 aagaagtcga cgatcatctg gctgcgctgg cgcgacgggg acggtcacga ccggacgatg
     3481 cccgtgtggt tcctctacga caacaaggcc cagaagatct acgtgatctc gggcgagcgc
     3541 cagcagacga tcccgggggc cgagcgcctg cgcgaggtcg acgtgatctt ccgctggaag
     3601 ggcaagaacg cccgcatcgg tgaggtgccg gctcgcgtca gggtcatgcc gcacggctcc
     3661 gagtgggaca cggtcgccga gaaggtggcg gagaagcgcc tcaacatccc cgggctgccg
     3721 gaggacacgg cccggcggtg gcgcgacgag tgcgtgctgc tggagctgac gctcctctcc
     3781 tgaggagcct ctcgctcagt tcgactggag cagcgcccgc cagtccttgg ccccggcctg
     3841 acgacggaag cgccggcgct cacgctcggt gaggccgccc cagatcccgt ggtcgacggg
     3901 ggcgtccatc gcataggcga ggcactggcc ctggaccggg cactcccggc agatgcgctt
     3961 cgcttgcttg tgctccatgg cgccgacggc gaaccagagg tcgggatccg tcttcgagca
     4021 cgagcccatg gcccgccaat cctgctccac caactgagtg ctcgacatgc tgcttccctc
     4081 cgggtgctga actcgcagaa cttttctagg accggggctc aagatagggc gccgggctgc
     4141 cgattccgat agcccgatcc gacccttttc cgggtgtcct cgcgggccgt acgccagtag
     4201 tcattccggg ccacgccccc cggcgtcgcc tacgcaggaa cgcctatttc aggggagaac
     4261 ctaggcttta gttaccgatc caatggcccc ggagtactcc tggaagatgc gacacctgcg
     4321 gtacctcctc gttgcgctgg cagtcggcgc gctctcggca gggtccctgc cgtcggccgc
     4381 cgctcaggcc gcgcccagct accagtcgag cgacccggcg aaggacgaga tgatggacca
     4441 cccgcccacg tcggtgaccg tgacgttcga ccagccgctg gaccagtcgt cgtggatgaa
     4501 cgtcctcgac gagtgcggga aggagatcga cgcgggggcc gccacggtcg acctgaacga
     4561 gatgacggtc gatatcggca agtcgccgtc ggggacgtac gaggtcgtct acaaggcggt
     4621 cggggtcgcc ggcgtgaccg gcgagtccgc gtcgagcttc gagttcatgg tccaccacgg
     4681 caagccgtgc ggggcgaaga agaaggacca ccaccacgac ccggacaaga agaagccgga
     4741 ccaccacgac cacggcgacg acgacgacgg gccgggccac gacgaccacg aggacatgga
     4801 catgggctct ggctcgggct cgtcggacca ctcggaccac ccgggcatgt ccgggagctc
     4861 gggctcgtcc ggccactcgg accactcgac ggcatcgacg ggtcacgagg gggccgggca
     4921 cggcgggcgc cacgggaacc acgaccccgg gaagccgggc gggcaggcga cgggcgccac
     4981 ggatccgccc gtgctggcga cgggcgacgg ggcggtgccc atcggagcgg acggccaggc
     5041 ggtgctcgta ggcctgggcc tggcgctggc ggaaggggtg ctgggtggct ggctgctgag
     5101 ggtttcgggg aacctggcct agccgagcac cttcaggttc gtgcaggaag cgagccggtt
     5161 gaagtcctcg tcggcgtgga gcaacggcac gtcgtctcgc acacacggcg ccgcgatcaa
     5221 acagtccagg gtcttgcgga tcgtcacccc cgctcgccgt gcagtgcggt agagctcggc
     5281 ggcgagagcg aaatcaccga gcgcttcgag ccgaaggatt gggaacgcct tcaagtgatt
     5341 ttcgaccaga cgcgcctctc gttcgtctcc gaggccctgc agtatctcgg tgaagacgac
     5401 gtcggtcaga gccaccggcg cgccgccctg gagcagctcg acgcagcgcg acgcccccgg
     5461 cgactcgaca tcgttgagga cgtcgatcca gatgcttgtg tcgacgacga tcacgtacgg
     5521 ccggagcggc tctcgtccag gttcccctcc caccggacct ttccccgaag gccgagcaaa
     5581 ccaaggcggc ggtgctgggc gacaacctcc cgtagcgcga agtcaacggc ggctcgtttc
     5641 gtcttcacgc ccgtcaggcg ctgcacctcg cggagcacgt cgtcgtcgat gtcgatgttg
     5701 gtacgcatac accataggat acacatacag cgttcattgt ctacacatca cggttgttcg
     5761 gccgttaccc ggctcggctc cactactacc tcccgggtcc ggctctggcc cgtcagccag
     5821 cccgtcaggc ccatcaggat caggcctatc gccagctccg ccgctatcgc cttgcggaac
     5881 gtcctcgtcg ccgagccggc ggacgacgac ttctccagcg ccgtctcgaa gcgcttgcgg
     5941 atcacgaagt ggttgaacgc ccccagggcg aggatgagcc cgaagaacag gatcttgatc
     6001 gtcagcgtgc ggccgtaggg ggtctccacg aggtcgccgg ggtgcccgac gtgcaggatc
     6061 gagccgacca tccccgtgac cagcacgatc cctatcgcca cgatcgccac gtgcgagaag
     6121 tgcctcacgg ccgggctcag cacctcgagc ctcgccgcgt cgtccttgcc cacccagctg
     6181 tccggcagcg ccaccgacaa gagcacgatc ccggcgaacc agatcgaccc cgccaccagg
     6241 tggacgacgt cggtcccgag cgagacgatc cgaggggtcg ccacggcggc gtggcccgag
     6301 aacgagctcg tcgccaggag cacgaccccc aggacggccc aggcccccca ccacgcaggc
     6361 cctgcgaccg acgagtcgac gccccgctgc gccagcgccc actgcctcac cttgcccgtc
     6421 aagagcacga gcagcccggc cacgatcggg atccgagccg cgtaccaggg gccgaagctc
     6481 gtctcgagga cggcgaagaa cgagtcggag gtcacatcgc cgcccagcag ctcggccgtg
     6541 agcaccgtct ggaggacgag cgcgagggcg gccgccgcgg cagccccgat cagagcgctg
     6601 tggacgagcc cctcgagccg ctcggccagc cgcgtacggc cccgggacca cgcctcggga
     6661 tcgagcgacg cgaacgtcgg acggaggacc agcacgacga ctgcaagggt cccgagcacg
     6721 agggcgttgg cggcgaatcc cgcgaaccgc gtgagcgtaa gcgccacttc ttggagcgtg
     6781 ctctcgaatt ccattacgat cgaaagcctc ccatgacgtc tgcgcagctc gtcctagtct
     6841 cggtgacgtc ggccctcatc gcggtcttcg cgatgctcgt ggtcgcgacg atcagacgtg
     6901 cccggacgcg cgacgaggaa tctaagaagg actagctgcg gaagccggct gcggcttcct
     6961 cgacggtcgg ggcgatcgag acgccctcgc cgaaccccgt gatcgtgaac agcttcagat
     7021 gctgctcgcg cgtgcacgcg atggccaggt cgccgccggc ctcgcggagc ctccggacgc
     7081 cgcccatcag cgcgccgagg ccggaggagt ccatgaaagg gacgccgtcg aggtcgacca
     7141 cgacgtgggg ggtcccgtcc tcgatcatcg tcccgatcgc gtcacggagc gacccgaccg
     7201 tgtagacgtc gaggtccccc tcgggccgca ggacctggta gtcgccctgg ttcctgacgt
     7261 cgatctcgat cttctgctgt gacatctctc tcctgacgtc tactccgcct cgggggcggc
     7321 cccgacccga gtcgccggcg gcaggtcgtc cgatccgggg gccgccgctc cctcgaactc
     7381 gatcacgagc atcgttaggt cgtcgtcgag ctgaccggct gcgaaccgct ccacgtcttc
     7441 gacgacgtgg cccgccaggt cgtccgaggg cagcgaggcg agctcgagga gcgacgcctc
     7501 gaggcgatgc tcgccgaaga cctcgccctc gggagacttc gcctcgagga acccgtcggt
     7561 gaacaggatc aggcggtcgc cgggggcgag cgtggcggtc gtctggttca acccggggtt
     7621 ctcgtcccac ccgagcggcc ggttgcgcgg cccctctgcc tcgacgaagt tgccgggaga
     7681 gccgccgtcg cgggcagggt cgggggcatg caggaggaac ggggccgggt gacccatgtt
     7741 cagccagcgc acctcgcccg tcacgatgtc gaccgtggcg tagacgcagg tcgtgaacgc
     7801 gccggtctcg gtcgcctcga gcagcgcgag gttcgcctgc tcgatcacgc gctt
//
LOCUS       DASQDO010000015         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_553711, whole genome shotgun sequence.
ACCESSION   DASQDO010000015 DASQDO010000000
VERSION     DASQDO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553711"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..356
                     /locus_tag="VEV43_00385"
     CDS             39..356
                     /locus_tag="VEV43_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HYP22010.1"
                     /translation="MGSREEVFAKPLHPYAWGLLESIPRIDQKGERLVPIEGAPPSLI
                     FVPPGCPFHPRCPHRFEPCDKERPDLVDRGGGHPDACHLTIENKRKLWAEREARRTKG
                     VPV"
     gene            386..1426
                     /locus_tag="VEV43_00390"
     CDS             386..1426
                     /locus_tag="VEV43_00390"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HYP22011.1"
                     /translation="MPRTSGDPLVEVRNLTKHFPITKGIILQREVARVHAVEDVTFSI
                     YPGETLGLVGESGCGKSTTARLVLRLLEPTSGSVIFDGQDITHVKRRDLRPIRREMQM
                     IFQDPYASLNPRQTVGQIIGAPYTIHKTEGETRKKVQDLMARTGLNPEHYNRYPHEFS
                     GGQRQRIGVARALALRPKLIVCDEPVSALDVSIQAQILNLLEDLQQEFKLTYLFISHD
                     LGVVRHIADRIAVMYLGRCVEVAQSDELYHRPRMPYTAALLSAVPKGHAGSNVAQRVV
                     LKGDVPSPIDPPPGCPFHPRCPKAREVGGGEIPENCRTQMPPLGEVDPRHFAACWYPV
                     EKGESLDKAAHA"
     gene            1443..1934
                     /locus_tag="VEV43_00395"
     CDS             1443..1934
                     /locus_tag="VEV43_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22012.1"
                     /translation="MAAVFVACLLSGCEPAVPAVVPYEPLELPEDVVREDTALTEQQV
                     KLRSDIVEVPDELVLGETLRYVVELTNESGTPVFLDPCPVYYQAWGESGFSVSRLSYL
                     NCDDAPPEIAPGASLRFAMELPIEDPEAVHVDGSIVWYLGSPFGGRKQDVGASGAIDV
                     VRR"
     gene            complement(1942..2424)
                     /locus_tag="VEV43_00400"
     CDS             complement(1942..2424)
                     /locus_tag="VEV43_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22013.1"
                     /translation="MHSRRVTQQMGYKVRKQTSEDVERPVVLFVCVHNAGRSRMAEAF
                     FNQLAGDRYRGMSAGTEPAGGPHPEVMEAMAEVHLPLEDTPGTSLTQEMTDGALLVVG
                     MGCDVEEACPALRVPLEDWGLDDPKGKSPDEVAEIRDMIEMRVRNLVARLDREKAAGS
                     "
     gene            2456..3316
                     /locus_tag="VEV43_00405"
     CDS             2456..3316
                     /locus_tag="VEV43_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HYP22014.1"
                     /translation="MTFSIAAWDGDVSPPEWGVAVASKFLAVGAAVPWVRAGAGAVAT
                     QALANLAYGPEGLDLLAAGTDAAGVVAQLTAADEGRDDRQLGVVDASGRAATFTGSSC
                     FDWAGGVAEDGFACQGNILTGPEVVDAMAGAFRATSGELAVRLLAALKAGDDAGGDRR
                     GRQSAALYVAREGGGYGGGVDRAVDLRVDDHLTPVDELARLWRIHSLLFPRPESLEFV
                     PIDGELAGEIRYLLSQLGYDAGSGGGDEPYDARLKGALYEYVGTENLEERWADDARIE
                     KRVLEHLRSH"
     gene            complement(3293..4459)
                     /gene="larC"
                     /locus_tag="VEV43_00410"
     CDS             complement(3293..4459)
                     /gene="larC"
                     /locus_tag="VEV43_00410"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HYP22015.1"
                     /translation="MKALHFDCFSGAAGDMILGALIDAGASREAVERALASLPLDGYS
                     FEVGEVTRASLRATKVDVIVTGAQEQRGPREIAQLLDAAPMPAPVRARAHHAFDLLAQ
                     AEAKVHGIPPERVHFHEVGAVDAIVDVVGCAAALESLKPERITCSPVATGRGTVEAAH
                     GPIPVPAPAVLELLKGVPLEERGTDELVTPTGAAILRAAVHEFGPMPPMTVAATGYGA
                     GTRDAALPNVVRVLVGEVSAMSSDETVLQIEANLDDMSPELLPHAIESLLAAGAVDAW
                     VTPVLMKKGRPGYVLAALAPAAERDRVVDVFLRETTTFGVRMHEAGREVLDRAWVEVD
                     VAGHTVRVKTAIRAGSVVTASPEYEDAHRVAKATGMPLKDVYERARASVRAQVL"
     gene            complement(4456..>5209)
                     /gene="larB"
                     /locus_tag="VEV43_00415"
     CDS             complement(4456..>5209)
                     /gene="larB"
                     /locus_tag="VEV43_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HYP22016.1"
                     /translation="DELEALLRSLIDGSTTVEETVAELQRLPLKDLGFARVDTHRELR
                     QGMPEAIYAEHKTASQVVAIAEALLAETTSAVIATRVPEETAHALLRRWPDALHDEAA
                     RLVVVRSTSDERAPAGTIAVVSGGTSDRPVALEAALTAEAVGIKVERIEDVGVAGLHR
                     VLAQEETLRTSDVVIVVAGMEGALPSIVGGLTPAPVIAVPTSVGYGASFGGLAALLGM
                     LNSCAAGVVVTNIDNGFGAALAALRQLNRGRA"
BASE COUNT          730 a         1871 c         1822 g          786 t
ORIGIN      
        1 atacggtcat gttcatttaa gcgggccgcg cctccgagct gggctcgcgc gaagaggtct
       61 tcgcgaagcc gttgcatccg tacgcgtggg gcctgctcga gtcgatccct cgcatcgacc
      121 agaagggcga gcggctcgtc ccgatcgaag gtgccccgcc gtcgctgatc ttcgtgccgc
      181 cgggctgccc cttccacccg cgttgcccgc accggttcga gccgtgcgac aaggagcggc
      241 ccgacctcgt cgaccgcggc ggcggtcacc ccgacgcctg ccacctcacg atcgagaaca
      301 agcggaagct gtgggccgag cgcgaggcgc ggcgcacgaa gggggtgccg gtgtgagcca
      361 ggtcgaggcg acgaggcccg ccggcatgcc gcgcacgtcg ggcgatccgc tcgtcgaggt
      421 ccgcaacctc acgaagcact tcccgataac gaagggcatc atcctgcagc gcgaggtcgc
      481 gcgcgtgcac gcggtcgagg acgtgacgtt ctcgatctac ccgggcgaga cgctgggcct
      541 cgtcggcgag tcgggctgcg gcaagtcgac gaccgcacgc ctcgtgctca ggctcctcga
      601 gccgacgtcg ggctcggtca tcttcgacgg gcaggacatc acccacgtga agaggcgcga
      661 cctgcggccg atccggcgcg agatgcagat gatcttccag gacccgtacg cgtcgctgaa
      721 cccgcggcag acggtcggcc agatcatcgg ggcgccgtac acgatccaca agaccgaggg
      781 cgagacgcgc aagaaggtcc aggacctgat ggcgcgcacg gggctgaacc ccgagcacta
      841 caaccggtac ccccacgagt tctccggggg ccagcgtcag aggatcggcg tcgcacgcgc
      901 gctcgcgctg cgcccaaagc tgatcgtctg cgacgagccg gtctccgcgc tcgacgtctc
      961 gatccaggca cagatcctga acctcctcga ggacctccag caggagttca agctgaccta
     1021 cctgttcatc tcgcacgacc tcggcgtcgt ccggcacatc gccgaccgca tcgccgtgat
     1081 gtacctcggc cgctgcgtcg aggtcgcgca gtccgacgag ctgtaccacc ggccgcgcat
     1141 gccatacacc gcggcgctct tgtcggcggt ccccaagggc catgcggggt cgaacgtcgc
     1201 gcagcgcgtc gtgctcaagg gcgacgtgcc gtcaccgatc gacccaccgc ccggatgccc
     1261 gttccacccg cgctgcccga aggcccgcga ggtcggcggc ggtgagatcc ccgagaactg
     1321 ccggacgcag atgccgccgc tcggcgaggt cgacccgcgt cacttcgccg cgtgctggta
     1381 ccccgtcgag aagggcgaga gcctcgacaa ggccgctcac gcctaggcgg ttcgcgcgtc
     1441 cggtggcggc ggtcttcgtc gcctgcctcc tgtccggatg cgaacccgcc gtccccgcgg
     1501 tggtgcccta cgagccgctg gagctccccg aggacgtcgt tcgcgaggac accgcgctga
     1561 ccgagcaaca ggtgaagctc cgcagcgaca tcgtcgaggt ccccgacgag ctggtgctcg
     1621 gagagacgtt gaggtacgtg gtcgagctca cgaacgaatc ggggaccccc gtcttcctgg
     1681 acccttgtcc cgtctactac caggcgtggg gcgagagtgg gttctcggta tcgcggctgt
     1741 cgtacctcaa ctgcgacgac gccccgccgg agatcgcacc gggcgcgtcg ctgcggttcg
     1801 ccatggagct tccgatcgaa gacccggagg ccgtgcacgt ggacggcagc atcgtctggt
     1861 atctcgggtc gccgttcggc ggacggaagc aggacgtggg cgcgtcgggc gcgatcgacg
     1921 tggtccggcg ttgagcgtcc gctagctccc ggcggctttt tcccggtcca gccgtgcgac
     1981 gaggttgcgc acccgcatct cgatcatgtc gcggatctcg gcgacctcgt ccggtgactt
     2041 ccccttcggg tcgtcgaggc cccagtcctc gagcgggacg cgcagcgcgg ggcacgcctc
     2101 ttcgacgtca cagcccatcc cgacgacgag cagggcgccg tcggtcatct cctgcgtgag
     2161 cgacgtgccc ggcgtgtcct cgagcgggag gtggacctcc gccatcgcct ccatcacctc
     2221 ggggtggggg ccgccggcgg gctcggtccc cgcggacatg cccctgtaac gatcaccggc
     2281 gagctggttg aagaacgcct cggccatacg cgagcgaccc gcgttgtgta cgcacacgaa
     2341 caggaccacg ggccgctcga cgtcctcgga cgtctgcttg cggaccttgt agcccatctg
     2401 ctgggtcacc cggcggctat gcatcttgga aggataggcg tgctgtaatg ctcgtgtgac
     2461 gttctcgatc gctgcatggg acggcgacgt ctcacctccc gagtggggcg tcgcggtcgc
     2521 gtcgaagttc ctcgccgtgg gggccgccgt gccgtgggtg cgcgccggtg ccggagcggt
     2581 cgcgacccag gcgctcgcga acctcgcgta cgggccggag ggcctcgacc tgctggctgc
     2641 cgggaccgac gccgcgggcg tcgtcgccca gctgacggcg gccgacgagg gccgcgacga
     2701 ccggcagctc ggagtcgtag acgcatccgg tcgcgcggcg acgttcacgg gctcgtcgtg
     2761 cttcgactgg gccggtgggg tcgccgagga cgggttcgcc tgtcagggca acatcctcac
     2821 ggggcccgag gtggtcgacg cgatggccgg cgcgttccgc gcgaccagcg gcgagctcgc
     2881 ggtgcggctc ctcgcggcgt tgaaggccgg cgacgacgcg ggcggggacc ggcgcgggcg
     2941 ccagtcggcc gcgctctacg tcgctcgcga gggcgggggc tacggcggag gcgtcgaccg
     3001 tgcggtggac ctgcgcgtgg acgaccacct gacgcccgtc gacgagctcg cgcgcctgtg
     3061 gcggatacac agcctgctgt tcccgcgtcc cgagagcctc gagttcgtcc cgatcgacgg
     3121 cgagctcgca ggcgagatca ggtacctgct gtcgcagctc gggtacgacg cgggctccgg
     3181 cggcggtgac gagccgtacg acgcccgcct caagggcgcg ctctacgagt acgtcgggac
     3241 ggagaacctc gaggagcggt gggctgacga cgcgcgcatc gagaagcgcg ttctagagca
     3301 cctgcgctct cactgacgcc cgcgcccgct cgtagacgtc cttgagcggc atcccggtcg
     3361 ccttcgcgac gcggtgcgcg tcctcgtact ccggcgacgc cgtgacgacc gatccggcgc
     3421 gaatcgcggt cttgacccgc acggtgtgcc cggcgacgtc gacctcgacc caggcgcggt
     3481 cgaggacctc gcggccggcc tcgtgcatcc tcacgccgaa cgtcgtcgtc tcgcgcagga
     3541 agacgtcgac gacgcggtcg cgctctgctg ccggcgcgag cgcggcgagg acgtagcccg
     3601 gccgcccctt cttcatgagc accggcgtca cccaggcgtc gacggcaccg gcggcgagga
     3661 ggctctcgat cgcatggggc aggagctcgg ggctcatgtc gtcgaggttc gcttcgatct
     3721 gcagcacggt ctcgtcggac gacatggccg agacctcgcc gaccaggacg cgaaccacgt
     3781 tcgggagcgc ggcgtcgcgc gtgccggccc cgtatcccgt cgcggccacc gtcatcggcg
     3841 gcatcggccc gaactcgtgg acggccgctc gaaggatcgc cgcgccggtc ggcgtcacca
     3901 gctcgtcggt gccgcgctcc tcgagcggga cgcccttcag gagctccagc actgcggggg
     3961 ccgggacggg gatcgggccg tgcgcggcct cgaccgtccc gcggcccgtg gctaccggcg
     4021 agcacgtgat cctttcgggt ttcagggact ccagcgcggc cgcgcatccg actacgtcga
     4081 cgatcgcgtc gaccgcgcct acctcgtgga agtggactcg ttcggggggg atgccgtgca
     4141 ccttggcttc tgcttgcgcc agaaggtcga acgcgtggtg cgcccgcgcc ctcacggggg
     4201 cgggcatggg ggccgcgtcg aggagctgcg cgatctctcg cggcccccgc tgctcttgcg
     4261 ctcccgtcac aatcacgtcc accttcgtcg cgcgcaggga cgctcgcgtc acctcaccga
     4321 cctcgaagga atatccgtcc aggggcaggg acgccagcgc tcgttcgacc gcttctcgtg
     4381 atgcaccggc gtcgatgagc gcgccgagga tcatgtctcc ggccgctccc gagaagcagt
     4441 cgaagtggag ggccttcacg cccggcctcg gttcagctgg cgcagcgcgg ccagggccgc
     4501 gccgaagccg ttgtctatgt tcgtcacgac taccccggcg gcgcaggagt tcagcatccc
     4561 caggagcgcc gccaggccgc cgaaggaagc gccgtagccg acgctcgtcg ggaccgcgat
     4621 cacgggggcc ggcgtcagac cgccgacgat gctcggcagc gctccctcca tccccgccac
     4681 gacgatcacc acgtcgctcg ttcgcagggt ctcctcctgg gccagcacgc ggtggagccc
     4741 cgcgacaccg acgtcctcga tccgctcgac cttgatcccg acggcctcgg cggtgagcgc
     4801 cgcctcgagc gcgaccggac ggtcggacgt ccctccgctc acgaccgcga tggtccccgc
     4861 cggtgcgcgc tcgtcgctcg tggagcgcac cacgaccagg cgcgccgcct cgtcgtgtag
     4921 cgcgtccggc caccgccgga gcagagcgtg cgcggtctcc tccgggaccc tcgtagcgat
     4981 caccgcggac gtcgtctccg ccagcagcgc ctcggcgatg gccaccactt gggacgcggt
     5041 cttgtgctcc gcgtagatcg cctccggcat cccctggcgg agctcgcggt gggtgtcgac
     5101 gcgtgcgaag ccgaggtcct tcaggggcag acgctggagc tcggccaccg tctcctcgac
     5161 ggtcgtcgac ccgtcgatca ggctcctgag cagggcttct agctcgtct
//
LOCUS       DASQDO010000016        12311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_737507, whole genome shotgun sequence.
ACCESSION   DASQDO010000016 DASQDO010000000
VERSION     DASQDO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737507"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VEV43_00420"
     CDS             <1..438
                     /locus_tag="VEV43_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22017.1"
                     /translation="LPAERAAAGRQIDGALEYVPKAAASLFVGDTVTHEHDLRLPLGR
                     PGDRAAAAIELSTDGYVRWLGRRLKEHDLPSLLVRAGGREWIAGKTEPEITAEAPSVF
                     DLLRALTGRRTRDEIAAFEWSADAEPYLDVFSMYGMPRAPLGE"
     gene            491..4132
                     /locus_tag="VEV43_00425"
     CDS             491..4132
                     /locus_tag="VEV43_00425"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HYP22018.1"
                     /translation="MDRQTEQETFGPNIWLVDEMYRQYQENPKAVSESWREFFEGYEP
                     GHAPKRAEEPRRPEPPRVQQAPPPPPVQQSQEATPIPDDASPLRGVAARIAENMEQSL
                     GIPTATSVRTIPAKLLEENRRIVNRYLAGKRGGKVSFTHLIGYAVLRALESRPAMKAS
                     FAEIDGKPHVVQHKHVNFGLAVDVERKDGGRILYVPNIKDADELDFAGFWSAYEQVIR
                     KVRANELAPEDFAGTTVTLTNPGTVGTRLSVPRLMPQQGLIVGTGAIGYPIEYEAADP
                     RSLARIGVSKVMTVTSTYDHRVIQGAESGQFLAAVHELLLGGDRFYDEIFASLKIPYK
                     PVRWSRDRSPEGVAELEKQSKVIQLINMYRVRGHLLAELNPIGWEVLAHPELDLSYYG
                     LTVWDLDRWFLTDGLPGGDRQPLRQIIDRLRDAYCRTTGIEYMHISEPDQKRWIQERV
                     EAEVDHELSVEDKKHILERLNAAEAFERFLHSKYTGQKRFSLEGAESLIPMLDALLEG
                     CCEHGAVEVVMGMSHRGRLNVLANIVGKPLNEIFNEFEGNIPPETVQGSGDVKYHLGM
                     TGRYTTRSGESIGVVLASNPSHLEAVDPVVEGMARAKQDMIGDGAHERVLPVLLHGDA
                     AFAGQGVVAETLNLSALPGYRTGGTVHVVVNNNIGFTTTPAAGRSSIYATDIAKMVQA
                     PIIHVNGDDPEACVRATQLALDFRNAFQKDVVIDLVCYRRYGHNEADEPAFTQPLMYS
                     KIEERRSVRKLYTESLLNRGELSVEEAEASLESFRERMQTSLKEVKESAPPKPVRAVP
                     PRPAGVLPPTDTAVNRERLDLIHRVLTSFPQGFEPHPKLKKMLEKRASMLNDDAIDWA
                     SGESLAFGSLLMEGIDVRIAGQDTRRGTFSQRHAVLVDYRTGEEYTPLNDLGDTGARL
                     RIYDSLLSEYAAMGFEYGYSVANGDALVCWEAQFGDFVNGAQIVIDQFLVAGEDKWGQ
                     ESGLVLLLPHGFEGQGPEHSSARLERFLTLSAEDNIQVVQPTTAAQYFHVLRRQMKRS
                     VRKPLVVMTPKSLLRHPAARSATDDLVSGGFAEALDDPFVSDRSAVKRVLLCTGKIAY
                     PLIEKRSADSSPAAIVRIEQLYPFPFEQLESIFTSYPNLEEIKWVQEEPENMGAWSFV
                     DARLWDRVKDRWKFTNASRFESGSPASGSALVHDQEHRELLEKAFDGLAQPGGESKPT
                     DAPPPPPSG"
     gene            4311..5102
                     /locus_tag="VEV43_00430"
     CDS             4311..5102
                     /locus_tag="VEV43_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP22019.1"
                     /translation="MGAVARHNISVRGPADGQPMLFAHGFGCDQNMWRFVYPAFEDDY
                     RIVLFDHVGAGRSDASSYDPEKYGSLRGYAADVLEICRELDLRDVVFVGHSVSSMIGV
                     LAANEEPGRFAALVLVSPSPAYIDDGDYVGGFSRADIDELLESLDSNYLGWSGAMAPV
                     IMGNGDRPELAEELTNSFCRTDPEIARRFAHVTFTADNRGDLPAVRVPSLVLQCRDDA
                     IAPEAVGRYVHEKIPGSRFALLDATGHCPNLSAPEPTIAAMKSFL"
     gene            5107..6285
                     /locus_tag="VEV43_00435"
     CDS             5107..6285
                     /locus_tag="VEV43_00435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYP22020.1"
                     /translation="MQPRPKSATAAADPYDAVPCGYLQTTPSGKIVRANRAFLEWTGY
                     AATDVVGQKSFVDLLTPGGRIFHETHYAPLLRMQGFAREIALDVVAADGRRLPALVNA
                     SVQTTDGASGADVAIAVFPAPDRREYERELLRARRQAEDSEAHARALARTLQSSLIPP
                     SPPLVPGVDVGAAFRPAGSGVEVGGDFYDVFETAAGVWGVVIGDVCGKGAEAAIVTAL
                     ARYTVRAAAMQAAQPHLVLGALNQAVLRQSPDRFCTVAYSTIRRGDPGYVCVTNASGG
                     HPLPVYVPARGDPRSIGRPGTALGLVPDPDLYDEEVRLDRGDVLAFYTDGVTEGRRER
                     EFFGESRLRDVLLEWRDRPATVIAEAIVDAVVAFQEAEPRDDIAVVVLKNPSTADLGS
                     "
     gene            complement(6370..7413)
                     /locus_tag="VEV43_00440"
     CDS             complement(6370..7413)
                     /locus_tag="VEV43_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP22021.1"
                     /translation="MTPSEGSSRRLTVAVTGPTGSIGRSFVRLLERTPEVERVLGMAR
                     RPFDPKRMGLRKLEYRRGDVLNRASVDALVEEADVVVHLAFLIMGSHDETEEINMKGS
                     RNVFESAFAAGVRRLVYTSSVAAYGFHKDNPEWLTEKVHARGSDNHYYSRQKAHLEEM
                     LSNLGRYADTDVYVFRPCIVAGPDSTDLIEQIPFVGAADKVPAPLRRLGGALPMLRPL
                     IPDPGIPFQLVHEGDVASALVAGVLGEGPPGTYNLAGDGEMTLSDLAHALGWYAVPVP
                     ELAVETAASIVSRLPLMPAKATWVNALRVPVLMDTTKAKKDLGWLPEFDVTETLMQTI
                     DSARTKGLLSRKA"
     gene            7458..8600
                     /locus_tag="VEV43_00445"
     CDS             7458..8600
                     /locus_tag="VEV43_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22022.1"
                     /translation="MPEVLRWIGCIAGLVLVLGTWIGMLDTLVVPRGLSSRTAAVVGR
                     VVRRLFLTLANRFDAYEKKDDVLVLQGPITLLALLVTWIVSFLLGYALILYPVENVSF
                     LTTVEETGSAFFTLGFAGPREAAATAIHVIAAFTGLIVIALQIAYLPTLYASFNRRET
                     LVTMLQSRAGSPAWGPEILRRHQAVNIVDNLPQLYAEWEQWAADVAESHTNYPVLVSF
                     RSPHPLRSWIVGLLAVVDSAALYSALCPSRAPSEARLCIRMGFNCMRDIADAMQIPFD
                     PDPLPTDPIELTFEEFRGAVHQLDEAGFPMERTPEEAWPHFRGWRVNYESVAYALADY
                     AVAPPGPWSGPREHLPGMAIIPQRPANRQPQDTREHERPRGEGTGW"
     gene            8597..8851
                     /locus_tag="VEV43_00450"
     CDS             8597..8851
                     /locus_tag="VEV43_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22023.1"
                     /translation="MTLDAGAALERVLAEGDEWQSWRALRLSGDATGDMPAIPYQDGE
                     GAFLGPGGTPSAGATGEGLCHLTVLGLADSGAAAIAADWL"
     gene            8933..9340
                     /locus_tag="VEV43_00455"
     CDS             8933..9340
                     /locus_tag="VEV43_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22024.1"
                     /translation="MWATAAAACGLMAVGRDPGPRAISLLRGEADSAGRFTGGGYPTF
                     AAAGAFWLAEGPKTEMAEWGLKWAREAEEDWWGPWERATALTFWAAAGIPYENATVEV
                     FVEELREAAPGQGWDDDLGLTLRTLELIDALGA"
     gene            9337..10275
                     /locus_tag="VEV43_00460"
     CDS             9337..10275
                     /locus_tag="VEV43_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HYP22025.1"
                     /translation="MTAQLTIPLRGPGGEPVDLARTLASHGVASLPPMALDEKSRSFE
                     VTLRVPKGLPRIVRVGPGRRDSVAIEVAGRAPGAATARSIEQQVRRILGLGQDLAPFY
                     EAIRDDPDLAWAAAGAGRMIRSPSVFEEVVKTICTTNCAWSATVRMTTALVEHLGEKG
                     AGAPAEGWRGRAFPAPEAMAAAGEDFYREVVRAGYRGRYLIALSEMVADGSLDLEALA
                     AATEDELPDDELAQRLQALPGVGPYAAAHVMMLIGRHSLLILDSWTRPTYARLTGKKK
                     VSDAAIERRFRRYGRYRGLAFWLFLTRGWVADEGAG"
     gene            10313..10825
                     /locus_tag="VEV43_00465"
     CDS             10313..10825
                     /locus_tag="VEV43_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYP22026.1"
                     /translation="MPANHPTGAGILSEEHREKRQELIGLLERAYWMEIETVMSYLAN
                     SINPDGVRAQEIIESLRADVQEELGHAQQFGERIKELYGVVPGSMEFSPEQKYLQPPA
                     SQTDIVHIIRGVIEAESGAIDHYNRIIEFCDGIDYVTQDMVIGILRDEEGHRRLFEGF
                     LREFESEGLA"
     gene            complement(10822..11880)
                     /gene="rsgA"
                     /locus_tag="VEV43_00470"
     CDS             complement(10822..11880)
                     /gene="rsgA"
                     /locus_tag="VEV43_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HYP22027.1"
                     /translation="MTQSFDLNELGWNPHFEQEMEPHRASGCVPGRVAVQYRGGYVVY
                     AESGEVQAILPGRMRHEDTDVPAVGDWVALRELPNEPGAGVVEAVLERTSKFSRHEAG
                     KVTREQVVAANVDVALLVSALNQDLNPRRLERYITTAWSGGTSPVIVLTKPDLAEDLG
                     AALAEAESVALGIPIHVVNGLTGDGIDEIAPYLAGNKTVVLLGSSGAGKSTLLNHLAG
                     GEVQRVQDIRHDGKGRHTTTVRHLFPIPGGGLVLDTPGMRELQLWVNDGMDQTFADVA
                     ELAQGCRFTDCTHEQEPGCAVIAAHEAGTLPAERLTSYRKLQRELQHLRLKQDRRAAA
                     EQKRQWKVLSKSYRRDFT"
     gene            12088..>12311
                     /locus_tag="VEV43_00475"
     CDS             12088..>12311
                     /locus_tag="VEV43_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP22028.1"
                     /translation="MSSRFTELCVDCHDPEKLAEFWCAVLGWSKGGSEPGLVEIKGDE
                     PGPTIVFVRVPEPKTVKNRVHIDVNPRDRE"
BASE COUNT         1928 a         4170 c         4362 g         1851 t
ORIGIN      
        1 ctgcccgcgg agagggccgc ggccggcagg cagatcgacg gcgcgctcga gtacgtcccg
       61 aaggccgccg cgagcctctt cgtcggcgac accgtgacgc acgagcacga cctccggctc
      121 ccactcgggc ggcccggcga cagggccgcg gccgcgatcg agctctcgac cgacggatac
      181 gtgcgctggc tcggccgcag gctgaaggag cacgacctgc cgtcgctcct cgtgcgagcc
      241 ggcgggcgcg aatggatcgc gggaaagacc gagccggaga tcacggccga ggccccctcg
      301 gtcttcgact tgctgcgggc gctcaccggg cgacgaaccc gtgacgagat cgccgcgttc
      361 gagtggagtg cggacgccga gccgtatctc gacgtcttct ccatgtacgg gatgccccgg
      421 gcgcctttgg gggaataggc cgggagtcgt tactctttcc tcaataagag accccgatca
      481 caaggacgtc gtggacaggc aaaccgagca ggagacgttc ggccccaaca tctggctggt
      541 cgacgagatg tatcgccagt accaggagaa ccccaaggcc gtcagcgagt cctggcgcga
      601 gttcttcgag gggtacgagc ccgggcacgc accgaagagg gccgaggagc cgaggcggcc
      661 ggagcccccc cgagtccagc aagcaccccc gccccctccc gtgcagcaat cccaggaggc
      721 gacgccgatc cccgacgacg cttcacctct gcgaggggtc gcggcccgca tcgccgagaa
      781 catggagcag tcgctcggca tcccgaccgc gacctcggtc cgcacgatcc cggcgaagct
      841 gctcgaggag aacaggcgga tcgtcaaccg ctacctcgcc ggcaaacgcg gcgggaaggt
      901 ctcgttcacg catctcatcg gctacgcggt cctgcgcgcc ctcgagtcgc gtcccgcgat
      961 gaaggcgagc ttcgccgaga tcgacggcaa gcctcacgtc gtccagcaca agcacgtgaa
     1021 cttcgggctc gcggtcgacg tcgagcgcaa ggacgggggc cggatcctct acgtcccgaa
     1081 catcaaggac gcggacgagc tcgacttcgc cggcttctgg agcgcgtacg agcaggtgat
     1141 ccggaaggtg cgcgcgaatg agctcgcgcc ggaggacttc gccgggacga ccgtgacgct
     1201 gacgaacccg ggaacggtcg ggacgcgcct ttccgtgcct aggctcatgc cgcagcaggg
     1261 cctcatcgtc ggcaccggcg ccattgggta cccgatcgag tacgaggccg cggacccgcg
     1321 ctcgctcgcg cgcatcggcg tgagcaaggt catgaccgtc acgtcgacct acgaccatcg
     1381 cgtgatccag ggggccgagt ccggccagtt cctcgccgcc gtgcacgagc tgctgctcgg
     1441 cggggatcgc ttctacgacg agatcttcgc ttcgctcaag atcccgtaca agcccgtgcg
     1501 ctggagccgc gaccgctcgc ccgagggcgt cgcggagctc gagaagcagt cgaaggtcat
     1561 ccagctcatc aacatgtacc gggtccgcgg ccacctgctc gcggagctca accccattgg
     1621 gtgggaggtc ctcgcccacc ccgagctcga cctcagctac tacgggctga ccgtgtggga
     1681 cctcgaccgc tggttcttga ccgacgggct gcccggcggc gaccgccagc ccttgcgaca
     1741 gatcatcgac cggctgcgcg acgcgtactg ccggacgacc ggcatcgagt acatgcacat
     1801 ctccgagccc gaccagaagc gctggatcca ggaacgcgtc gaagccgagg tcgaccacga
     1861 gctgtcggtc gaggacaaga agcacatcct cgaacggctg aacgcggccg aggccttcga
     1921 gcggttcctg cactcgaagt acacggggca gaagcgcttc tcgttggagg gggccgagag
     1981 cctcatcccg atgctcgacg cgttgctcga gggctgctgc gagcacggtg ccgtcgaggt
     2041 cgtgatgggg atgtcgcacc gcggccgcct caacgtgctg gcgaacatcg tcggcaagcc
     2101 gctcaacgag atcttcaacg agttcgaggg caacatcccg cccgagaccg tgcagggatc
     2161 gggcgacgtc aagtaccacc tcggcatgac aggccgctac acgacgcgaa gcggcgagag
     2221 catcggcgtc gtcctcgcgt cgaacccgtc acacctcgag gccgtcgatc ccgtcgtcga
     2281 gggcatggcg cgcgcgaagc aggacatgat cggagacggt gcgcacgagc gcgtgctccc
     2341 ggtgctgctg cacggcgacg cggcgttcgc ggggcagggg gtcgtcgcgg agacgttgaa
     2401 cctgtcggcg ctccccggct atcgcaccgg cgggaccgtc cacgtcgtcg tcaacaacaa
     2461 catcgggttc acgacgacgc cggcggccgg ccgttcgagc atctacgcga ccgacatcgc
     2521 caagatggtc caggccccca tcatccacgt caacggggac gatccggagg cttgcgtgcg
     2581 cgcgacgcag ctcgcgctcg acttccgcaa cgcgttccag aaggacgtcg tgatcgacct
     2641 cgtctgctac cggcgctacg ggcacaacga ggccgacgag ccggcgttca cgcagccgct
     2701 catgtactcg aagatcgagg agcgccgctc ggtgcggaag ctctacacgg agtcgctgct
     2761 gaaccgcggc gagctgagcg tcgaggaggc cgaggcctcg ctcgagagct tccgcgagcg
     2821 catgcagacc tctttgaagg aagtgaagga gagcgcgccg ccgaagccgg tccgcgcggt
     2881 gccgcctcgt cccgcggggg tcttgccccc gaccgacacg gcggtgaacc gcgagcggct
     2941 cgacctgatc caccgcgtgc tcacgtcctt cccgcagggg ttcgagccgc atcccaagtt
     3001 gaagaagatg ctcgagaagc gggcgtcgat gctgaacgac gacgcgatcg actgggcgtc
     3061 gggtgagtcg ctcgcgttcg gctccctgtt gatggagggg atcgacgtca ggatcgccgg
     3121 acaggacacg cggcgcggca ccttcagcca gcgccacgcc gtcctcgtcg actaccggac
     3181 gggcgaggag tacacgcctc tgaacgacct cggcgacacc ggcgcgcgcc tgcgcatcta
     3241 cgacagcctg ctcagcgagt acgccgcgat gggcttcgag tacgggtact cggtggcgaa
     3301 cggcgacgcg ctcgtgtgct gggaggcgca gttcggcgac ttcgtcaacg gcgcgcagat
     3361 cgtgatcgac cagttcctgg tcgccggcga ggacaagtgg gggcaggaga gcggcctcgt
     3421 gctgctgctc ccgcacgggt tcgaggggca ggggccggag cactcctcgg ctcgattgga
     3481 gcggttcctc acgctgagcg ccgaggacaa catccaggta gtccagccga cgaccgccgc
     3541 gcagtacttc cacgtgctgc gccggcagat gaagcgctcg gtgcgcaagc cgctggtcgt
     3601 gatgacgccg aagtcgctgc tacgccatcc ggcggctcgg tccgcgacgg acgacctcgt
     3661 gtcgggcggg ttcgccgagg cgctggacga cccgttcgtg tcggatcgtt cggccgtcaa
     3721 gcgtgtcctg ttgtgcaccg gcaagatcgc ctacccgctg atcgagaagc gttcggccga
     3781 ctcgtcgccc gcggcgatcg tgcggatcga gcagctctac ccgttcccgt tcgagcagct
     3841 cgagtcgatc ttcacgagct atccgaacct cgaggagatc aagtgggtcc aggaggagcc
     3901 cgagaacatg ggggcctggt cgttcgtgga cgcccggctg tgggaccggg tgaaggaccg
     3961 gtggaagttc acgaacgcgt cgaggttcga gagcgggagc ccggcgagcg gctcggccct
     4021 ggtccacgac caggagcacc gggagctgct ggagaaggcc ttcgatggcc tggcccagcc
     4081 ggggggcgag agcaagccca ccgacgcgcc gccgccacct ccctcgggct agccgcgggt
     4141 cgggtcgtcc cttagcagcc gcaacgcggt ccggtcgacg agcgcatgga ccgtgaacgt
     4201 ctggcctgcc gaacgcgccc ccgtgcccga gcgcttccac ggacggaagg ataggcggga
     4261 ggagaacggg ggttagggcg gcactccgaa gcccggcgta ggattcgccg atgggggcgg
     4321 tcgcgcggca caacatctcc gttcgggggc cggcagacgg tcagccgatg ctgttcgcgc
     4381 atgggttcgg atgcgaccag aacatgtggc ggttcgtgta cccggccttc gaggacgact
     4441 atcggatcgt cctcttcgac cacgtcggag ccggacgctc cgacgcctcc tcgtacgacc
     4501 cggagaagta cgggtcactg cgaggatacg cggcagacgt tctcgagatc tgccgcgagc
     4561 tcgatctccg cgacgtcgtc ttcgtcggcc actcggtgag ctcgatgatc ggagtgctcg
     4621 cggcgaacga ggagcccggg cggttcgccg cgctcgtgct cgtgtctccg tcaccggcgt
     4681 acatcgacga cggcgactac gtcggcggat tcagccgtgc cgacatcgac gagctgctgg
     4741 aatcactaga cagcaactac ctcgggtggt ccggcgcgat ggcaccggtg atcatgggga
     4801 acggcgaccg tcccgagctt gcggaggagc tgacgaacag cttctgcaga accgatccgg
     4861 agatcgcccg gcggttcgcg cacgtcacgt tcacggccga caaccgtggg gaccttccgg
     4921 cggtccgggt gccgtctctc gtgctccagt gccgcgacga cgcgatcgcg cctgaggcgg
     4981 tcggccgcta cgtccacgag aagatccccg ggagccggtt cgcgctgctc gacgcgacag
     5041 ggcactgtcc gaacctcagc gcaccggagc cgacgatcgc cgctatgaag tcgttcttgt
     5101 aaggacgtgc agccgcgccc gaagtctgcg accgccgccg ctgatccgta cgacgccgta
     5161 ccgtgcgggt acctgcagac gacccccagc gggaagatcg tccgagcgaa ccgtgccttc
     5221 ctcgaatgga cgggttacgc ggcgaccgac gtcgtggggc agaagagctt cgtcgacctg
     5281 ctcacgccgg gagggcggat ctttcacgag acgcactacg cgccgctgct tcggatgcag
     5341 ggattcgcca gggagatcgc gttggacgtg gtggcggcgg acggccgccg gcttccggcg
     5401 ctggtcaacg cctcggttca gacgacggat ggagcgtcgg gcgccgacgt cgcgatcgcc
     5461 gtgttcccgg cgccggaccg gcgggaatac gagcgcgagc tgctgcgggc gcgacgacag
     5521 gccgaggact cggaggcgca cgcacgcgct ctcgcccgga cgctgcagtc gagtctcatc
     5581 cctccgtcgc cgccgctggt gccgggagtc gacgtcgggg ccgcgttccg tcctgcgggg
     5641 tcgggcgtcg aggtgggagg cgacttctac gacgtattcg agaccgccgc gggcgtatgg
     5701 ggcgtcgtga tcggcgacgt gtgcgggaag ggggcggaag ctgcgatcgt cacggcgctg
     5761 gcgcgctaca ccgtccgggc cgcagcgatg caggcagcgc aaccgcatct cgtgctcggg
     5821 gccctgaacc aagcagtgct gcggcagagt ccggaccggt tctgcaccgt cgcctactcg
     5881 accatcaggc gcggcgaccc cggctatgtc tgcgtcacca acgcgtccgg cgggcatccg
     5941 ctgcccgtct acgtgccggc acgcggcgac ccccgatcca tcggccggcc tgggaccgcg
     6001 ctgggcctgg tccccgaccc cgacctctac gacgaggaag tgaggctcga ccgcggcgac
     6061 gtcctggcct tctacaccga cggcgtgacc gaggggaggc gagagcgtga gttcttcggc
     6121 gagtcgcgat tgagagatgt cctcctggag tggcgggaca gaccggcaac cgtcatcgcc
     6181 gaggccatcg tggacgccgt cgtcgctttc caggaggcgg agccgaggga cgacatcgcg
     6241 gtcgtggtgc tgaagaaccc gtcgaccgca gatctcggtt cgtgacgtct cggctcggaa
     6301 ggccccgccc tgtaacccct cccagaacac gacgcggtag gcgtggcccg tgcatggctt
     6361 cgaggagggc taggcctttc ggctgagcag gcccttcgtg cgggccgagt cgatcgtctg
     6421 catcagggtc tccgttacgt cgaattccgg gagccagccc aggtctttct tcgccttcgt
     6481 cgtgtccatc aggaccggga ctcgcagcgc gttcacccag gtcgccttcg ccggcatcag
     6541 tgggagccgg gacacgatcg acgccgcggt ctcgaccgcc agctccggca ccggcaccgc
     6601 gtaccagccc agcgcgtgcg ccaggtccga cagcgtcatc tcgccgtcgc ccgcgaggtt
     6661 gtacgtcccc ggcgggccct cgccgagcac tccggccacg agcgccgaag ccacgtcgcc
     6721 ctcgtggacc agctggaacg ggatgccggg gtcggggatg agcgggcgca gcatcggcaa
     6781 cgcgccgccg agccgccgca acggcgccgg gaccttgtcc gcggccccca cgaacgggat
     6841 ctgctcgatc aggtccgtcg agtccggccc ggccacgatg cacggccgga acacgtagac
     6901 gtccgtgtcc gcgtagcggc cgaggttcga cagcatctcc tcgaggtgcg ccttctggcg
     6961 tgagtaatag tggttgtcgc tgcctcgtgc gtggaccttc tcggtgagcc actcggggtt
     7021 gtccttgtgg aacccgtacg ccgcgaccga cgacgtgtac acgagacgcc ggacgccggc
     7081 tgcgaaggcg gactcgaaga cgttgcgcga gcccttcatg ttgatctctt cggtctcgtc
     7141 gtgcgagccc atgatcagga acgcgaggtg cacgacgacg tcggcctcct cgacgagcgc
     7201 gtcgaccgac gcccggttca gcacgtcccc gcgccggtat tcgagcttgc gcaatcccat
     7261 gcgcttcggg tcgaacggac ggcgcgccat gccgaggacg cgctcgacct ccggcgtgcg
     7321 ctcgaggagc cggacgaacg agcggccgat gctcccggtg ggcccggtga cggcgacggt
     7381 gagacggcgg gacgagcctt ccgagggggt cacgcaccta gtattcccct cgtgagccga
     7441 gcccgaggac gaccctgatg cccgaggtgc ttcgttggat cgggtgcatc gcgggcctcg
     7501 tcctggtgct ggggacctgg atcggcatgc tcgacacgtt ggtcgtccca cgcgggctct
     7561 cgtcgcgcac cgcggccgtg gtggggcgcg tcgtgcgccg gctgttcctc actctcgcga
     7621 accggttcga cgcgtacgag aagaaggacg atgtgctcgt gctccagggg ccgatcacgt
     7681 tgctggcgct gctcgttacg tggatcgtct cgttcttgct cggctacgcg ctgatcctct
     7741 atccggtcga gaacgtgtcc ttcctgacga cggtcgagga gaccgggtct gcgttcttca
     7801 cgctgggttt cgccggccct cgtgaggccg ccgcgaccgc gatccacgtc atcgccgcgt
     7861 tcacggggct gatcgtgatc gcgctccaga tcgcgtacct gccgaccttg tacgcgtcgt
     7921 tcaaccggcg cgagacgctc gtgacgatgc tacagagccg ggccgggtcg cccgcgtggg
     7981 ggccggagat cctgcggcgt caccaggcgg tgaacatcgt cgacaacctg ccccagctgt
     8041 acgcggagtg ggagcagtgg gccgccgacg tcgccgagag ccacacgaac tacccggtcc
     8101 tcgtgtcgtt ccggtcgccg cacccgctgc gctcgtggat cgtcggcctg ctcgcggtcg
     8161 tggactccgc cgcgctctac agcgcgctgt gtccgtcgcg tgctccgtcg gaggcgcgtc
     8221 tgtgcatcag gatgggcttc aactgcatga gggacatcgc cgacgcgatg cagatcccgt
     8281 tcgaccccga cccgcttccg accgacccga tcgaactcac gttcgaggag ttccgcggcg
     8341 cggtgcatca actggacgag gccggcttcc cgatggagcg gacgccggag gaggcgtggc
     8401 cgcacttccg cggctggcgc gtgaactacg agtcggtcgc gtacgcgctc gccgactacg
     8461 ccgtcgcgcc gccggggccg tggtccggcc cccgcgagca cctccccggc atggcgatca
     8521 tcccgcagcg cccggcgaac aggcagcccc aggacacgcg cgagcacgaa cggccacgag
     8581 gcgaggggac gggctggtga cgctcgacgc gggcgcggcc ctcgaacgcg tgctggccga
     8641 aggcgacgag tggcagtcgt ggcgcgcgct gcgtctgtcg ggcgacgcaa ccggcgacat
     8701 gccggcgatc ccgtaccagg acggcgaggg cgcgtttctc gggccgggcg ggaccccgtc
     8761 tgccggcgcc accggcgaag gtctgtgtca tctcacggtg ctcggcctcg cggactcggg
     8821 cgcggctgcg atcgcggcgg actggctgta gcaggcgcgc acgcccgcgc tcgcgtggct
     8881 cgatcctccc gacgaggttc cgggcgagct cgacaacccc gcggcctcgc gcgtgtgggc
     8941 gacggcggcc gccgcgtgcg ggctgatggc ggtcggacgc gatcccggcc cccgtgcgat
     9001 cagcctcctg cgcggggagg ccgactccgc agggcgcttc accggcggcg gctacccgac
     9061 gttcgccgcg gcgggcgcgt tctggctcgc cgagggcccg aagaccgaga tggcggagtg
     9121 ggggctcaag tgggcccggg aagccgaaga ggattggtgg gggccgtggg agagagcgac
     9181 cgcgctcacg ttctgggccg cggccgggat cccctacgag aacgcgaccg tcgaggtgtt
     9241 cgtcgaggag ctgcgtgagg ccgctccggg gcagggctgg gacgacgacc tcggcctgac
     9301 cttgaggacg ctcgagctga tcgacgcgtt gggcgcatga ccgcgcagct gaccatcccg
     9361 ctgcgggggc cgggcggcga gcccgtcgac ctggcgcgca cgctcgcgtc gcacggcgtc
     9421 gcgtcgctgc ccccgatggc cctcgacgaa aagtcgcgct cgttcgaggt gaccttgcgc
     9481 gtgccgaagg gccttccgcg gatcgtgcgc gtaggcccgg gccgacgcga cagcgtcgcg
     9541 atcgaggtcg cgggtcgcgc tccgggggcg gcgaccgctc gatcgataga gcagcaggtc
     9601 cggcgcatcc tcgggctcgg ccaggacctc gcgccgttct acgaagcgat ccgcgacgac
     9661 ccggacctcg cgtgggccgc cgccggcgcg ggccggatga tccgcagccc gtcggtcttc
     9721 gaagaggtcg tgaagacgat ctgcacgacg aactgcgcgt ggtccgcgac cgtccggatg
     9781 acgaccgcgc tggtcgagca tctcggcgag aaaggggcgg gcgcgccggc cgagggatgg
     9841 cgagggcgcg cgttcccggc tcccgaagcc atggccgcgg cgggggagga cttctaccgc
     9901 gaggtcgtgc gcgccggcta tcgcgggagg tacctcatcg cgttgtccga gatggtcgcg
     9961 gacgggtcgc tcgacctcga agcgctcgcg gcggcgaccg aggacgagct gcccgacgac
    10021 gagctcgcgc agcgtctcca ggccctcccg ggcgtcggcc cctacgcggc tgcgcacgtc
    10081 atgatgctga tcggccggca ctcgctgctg atcctcgact cgtggacccg gcccacctac
    10141 gcccgcctga ccggcaagaa gaaggtgagc gacgccgcca tcgagcgtcg cttccggcgc
    10201 tacggacgtt accggggact cgcgttctgg ctgttcctga ccaggggatg ggtggccgac
    10261 gagggggccg ggtagagaac ccggaagacc aaacgaccca aggaggccga agatgcccgc
    10321 caaccacccc accggcgccg ggatcctgtc cgaggagcac cgcgagaagc gtcaggagct
    10381 gatcggcctg ctcgagcgcg cgtactggat ggagatcgag accgtgatga gctacctcgc
    10441 gaactcgatc aaccccgacg gcgtgcgagc ccaggagatc atcgagagcc tccgcgccga
    10501 cgtccaggaa gagctcggcc acgcgcagca gttcggcgag cgcatcaagg agctgtacgg
    10561 cgtcgtgccg ggctccatgg agttctcgcc cgagcagaag tacctgcagc cgccggcgag
    10621 ccagaccgac atcgtgcaca tcatccgggg cgtgatcgag gccgagtccg gtgcgatcga
    10681 ccactacaac cgcatcatcg agttctgcga cgggatcgac tacgtcacgc aggacatggt
    10741 gatcggcatc ctgcgcgacg aagaaggcca ccggcgcctg ttcgagggct tcctgcgcga
    10801 gttcgagtcc gagggcctcg cctaggtgaa gtctcggcgg tagctcttcg acagcacctt
    10861 ccactggcgc ttctgctcgg ctgccgcgcg gcggtcttgc ttcaggcgta ggtgttgcag
    10921 ctcgcgctgg agcttgcggt agctcgtcag tcgctcggcc ggcagcgttc ctgcctcgtg
    10981 tgccgcgatc accgcgcagc ccggctcctg ctcgtgcgtg cagtcggtga aacggcagcc
    11041 ctgtgcgagc tcggctacgt cggcgaacgt ctggtccatg ccgtcgttca cccatagctg
    11101 gagctctcgc atgcccgggg tgtcgaggac caggcccccg ccggggatcg ggaacagatg
    11161 gcgcacggtc gtcgtgtggc ggcccttgcc gtcgtgacgg atgtcctgca cgcgctggac
    11221 ctcgccgccg gcgaggtggt tgagcagcgt cgacttgccc gcgcccgacg acccgagcag
    11281 cacgacggtc ttgttgccgg cgaggtacgg cgcgatctcg tcgatcccgt cgcccgtgag
    11341 cccgttgacg acgtgtatcg ggatgccgag cgcgacggac tcggcctccg cgagcgcggc
    11401 ccccaggtcc tcggcgaggt ccggcttcgt cagcacgatt accggtgacg tcccgcccga
    11461 ccacgcggtc gtgatgtagc gctcgaggcg gcgcgggttc aggtcctggt tcagggccga
    11521 gaccagcagg gcgacgtcga cgttcgcggc gacgacctgc tcgcgtgtga ccttgccggc
    11581 ctcgtggcgc gagaacttgc tcgtgcgctc gagcacggcc tcgacgacgc cggcgccggg
    11641 ctcgttgggg agctcgcgca gcgccaccca gtcgccgacg gcgggcacgt cggtgtcctc
    11701 gtgccgcatg cgtcccggaa ggatcgcctg cacctcgccg gactcggcgt agacgacgta
    11761 gcccccgcgg tactgcaccg ctacccggcc ggggacacag ccggaggcgc ggtggggttc
    11821 catctcttgc tcgaagtggg ggttccaccc cagctcgttc agatcgaagc tctgggtcaa
    11881 ctcgactcct cgtgtaggcg cgacgggatt cagggggccg gacgcgcggg agtcgctgcg
    11941 agaaagagct agcgaacggg accgtgcgcc ggcgcggcgg ggttcacggc aggtgcagtc
    12001 atcaattccc cacctccttc gcgggcgacg gtcgacttgt cagaggatga acggtcacgc
    12061 ggagaaaagc aagcacgagg aggcgggatg agcagcaggt tcacggagct gtgcgtggat
    12121 tgtcacgatc ccgagaagct cgcggagttc tggtgcgccg tgctcgggtg gagcaagggc
    12181 gggtccgagc cgggcctggt cgagatcaaa ggagacgagc ccggccccac gatcgtgttc
    12241 gtccgggtgc ccgagccgaa gacggtcaag aaccgcgtgc acatcgacgt caacccccgc
    12301 gaccgcgaac a
//
LOCUS       DASQDO010000017         8759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_859906, whole genome shotgun sequence.
ACCESSION   DASQDO010000017 DASQDO010000000
VERSION     DASQDO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859906"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VEV43_00480"
     CDS             complement(<1..351)
                     /locus_tag="VEV43_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYP22029.1"
                     /translation="MSMQSRRDFLKVALAGGAALALPRPALALADAFAPVEPFQVPLR
                     IPPVLTPVRKAGGRDYFVTTMRPARPRLLKGKRRTPVWSFDGTFPGPTIKVTRGHEAI
                     VRRVNELDVPVTIHL"
     gene            507..1727
                     /locus_tag="VEV43_00485"
     CDS             507..1727
                     /locus_tag="VEV43_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22030.1"
                     /translation="MPPRISLCLLACATAAALLPASPAHAALTFEVEVGRFFDEADHT
                     AESMRFYPSAIEIAPGDTLHFTTESFHGVTLLPAGVEAGPWAGEHATAGGTWSAFVAD
                     PDEGRRGAKMNLRVASPSDPCGWPGQSPCEFDGRGDEMVDPLNSGVALFPSESGVETR
                     ELSFSVTITADPGSTIFAVDPLHPAMTMRIDVVGSFAERSDLGELQAASAAQFAADAD
                     RADALDRTYSRKRVKKTVRGKTVWQAWAGVEEQGISLRRFYPRKLTIAPGNRVKWVFS
                     KDLYEAHAVTFPRTRAQAMARAFPEIACDLDGDEGTGDDFAPTSAEFPFCSSGELEVD
                     VPASFVGPAGNGSFRGGRDFETSGARGASFATTKRAYQLTFPKKSPRAGFAYACPIHE
                     AAHAPMNAAVVVKR"
     gene            1724..4006
                     /locus_tag="VEV43_00490"
     CDS             1724..4006
                     /locus_tag="VEV43_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HYP22031.1"
                     /translation="MSPRLKLSLLAVAAVAALAGCELEKDPSATPPPAPTPPPDVTSP
                     QGASNPSPDRLIFSSGRDEPDLELYSSFPDGSARKRLTRNTEGESGVVAYPNTGLVYY
                     VCGRAESICVATTSGTGTAGVLSSQRLGFPIIEDPAISPDGSQMALTAVKISPDGSST
                     NYDIYIYDFATEEFENFAVGPALDQMPAWVSDDTIVWSRFRNGDWELVSFRLDGPPGS
                     APAVLTNNDVDDLGVDVSRDGTKLAWIGTRRDAPNAGELFTMPVTGGTPTSLEPVRLE
                     RGFIGGDPDVAWSPDGTEIAFAGLAPGEDDVEIFTIPAADGPVTNVTRNDVYDVDPEW
                     ATLPPSLSVASPLLFTEGGPGGVVFEILLDAPQGEVVRVDYTTVPGTATAADFTPRSG
                     TAVFQPGETRVAVTVPVTDDTQIEPAETFSLRLSNSSAGTLTANDQATARIMDDEVEP
                     TPTPPPTATPTPGPTSSPGPVTDGRIAFASTRNGPQQIFTMASDGTDVRHVSPDQSSS
                     ELGSPNWSPDATRLIHTAMNNSGGAPQPEIVDRPSDGSGSFRILQVGPSVDSDPVYLP
                     GDPLGGYVFTSDRDGDFEIYYATPSGGFTKKLTDNSANDGHPSFSAASNGMVRMVFHS
                     NADGDFDVYRLDVSTSGDPSGSPVNLTPEGAGQAPHDELAPDFALGRNKITYHGNEHG
                     DFDVYALDLGTMQESHLTTDVANETHPAFSPTGNFIAFQKDLGGGNFEIFVMSASGGA
                     GTNVTNSPGADLTPEWGTGR"
     gene            complement(4010..4600)
                     /locus_tag="VEV43_00495"
     CDS             complement(4010..4600)
                     /locus_tag="VEV43_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYP22032.1"
                     /translation="MSLRDNKKTRTRETLRTAALRLFREKGFERTTVDEIAAEAGVSR
                     TTFFRYYPTKESVVFDRGRELGEVFRRWIAERPRKENPFEAFEGALLALARESQSSGL
                     AQESLELEELFERNPALRKSRAEHRQEQIALVAQALADRDGGPVEAEHRLAADIGLVV
                     SELVREQWHASGAQIDVEALLRDLFATVRALTGAGR"
     gene            complement(4796..5173)
                     /locus_tag="VEV43_00500"
     CDS             complement(4796..5173)
                     /locus_tag="VEV43_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYP22033.1"
                     /translation="MSKRRSLIGAVAFVALLGAACGGDDGGDDGGAAAGGNGGNGGSI
                     TLTAADFAWEPGSLTASAGSSIELVNEDEAKHNLTIEDAGVDEDVEGGASVTVDLGDV
                     EAGSYEFVCEYHPDTMTGTLEVQ"
     gene            complement(5185..5940)
                     /locus_tag="VEV43_00505"
     CDS             complement(5185..5940)
                     /locus_tag="VEV43_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP22034.1"
                     /translation="MQKTPQVLVVVALLGLAACDPAPPGAQSSQPEPQRVRRQAAAEP
                     ARPKPPKRVDPRADGFEVVLGEWAVTPEAPAIRPGRVTFVVHNRGTMGHGYEMELEGD
                     SSGPGSGDLFKAESEVVRPGESTRLTLTLPPGLYEIECLVDGHDDMGMEGVLEVRADA
                     PLVKAEDEARPGRIAIADFAFEPATAVVPAGTEVTWRNADPADHTVTSPDGDFGSDAL
                     GTGESFSHRFDEPGSYRYQCAIHPEMKGKVKVE"
     gene            5990..7381
                     /locus_tag="VEV43_00510"
     CDS             5990..7381
                     /locus_tag="VEV43_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYP22035.1"
                     /translation="MPIEHVSLDPSRDEMEAIARAAVGYAIDFVEARSTAPAVDVDGA
                     YELAEKLRSAPPEEPAEFGELLDVIARAADAAFDTAGPGYLAYIPGGGLYAAAVADLV
                     ACVTNRFVNLAAPAPALVRLEADVARWLCDLFEFPPEAQGVLTSGGSMANFSAVVTAR
                     TVCLPEDFLSGTIYVSEHVHHSIAKSARLAGFPPAALRPVRTDTSLRLDVDDLREQVA
                     RDRAEGRRPFLVVGSAGTVNTGAVDPLDEIAAFADEEGLWFHVDGAYGGFFQMTERGK
                     ERFRGIERADSVTLDPHKGLFLPYGTGSLIVRRGGDLLAAHRGGASYLPAQSETDLPD
                     FADYTPELSRDNRGLRVWVPLHLHGVGAFRRALDEKLDLAAKVYDALVDVDDLDVPWE
                     PQLSIVAFKSRRGNEATDRILQDVNASKRVYLSGTELEGERYLRVAVLSHRTDESRID
                     EAIEIISKAAANS"
     gene            7396..8019
                     /locus_tag="VEV43_00515"
     CDS             7396..8019
                     /locus_tag="VEV43_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HYP22036.1"
                     /translation="MSDLLTANLLVVNQKAKLIEMTNQYAIRDADGNELGRIEQEGQS
                     KLKKAVRLVGGFDQFMTHTLGVYDAGGNRVLQLTRPRKVFKSRLEVKDSGGRKVGEIV
                     QKNIFGKISFDFVDPNGRSVGRIKAENWRAWNFAIVDDTDREIGRITKKFVGALKGMF
                     TTADNYVVEIDPSVTGDLRLFALGAAAGVDTALKQDDRGLNIGGIGG"
     gene            complement(8024..>8759)
                     /locus_tag="VEV43_00520"
     CDS             complement(8024..>8759)
                     /locus_tag="VEV43_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22037.1"
                     /translation="VSAFLTATPHKAVALAGVAALAAPFARKLASQWGVVFNTVGAVS
                     LAGTMTASAFTGVAAVERPVAQRLEGRDKPRAVQPAADETAPDAANGDSPDPAGNAFG
                     HDKKAREGGNGKSQAGRGGNALGHGKKAKALGHAHDAEGTPNGKAKGHSKPDKPEKVK
                     PEKPAKPEKPTKPEKPATAAKPEKPAGPTGDAAGPPPAKEKPAPVEEPPAHAEPAEPD
                     PAPAPEAPVPEPAPKTPPGKAKGKSK"
BASE COUNT         1251 a         3244 c         2947 g         1317 t
ORIGIN      
        1 caggtggatc gtcacgggca cgtccagctc gttgacgcgc cgcacgatcg cctcatgtcc
       61 gcgggtcacc ttgatcgtcg ggcccgggaa cgtgccgtcg aagctccaga cgggcgtccg
      121 ccgcttcccc ttcaacaggc gggggcgggc cgggcgcatc gtcgtgacga agtagtcgcg
      181 gccgccggcc ttgcgcacgg gcgtcaggac cggagggatc cgcagcggga cctggaacgg
      241 ctcgacggga gcgaacgcgt ccgcgagagc gagggccggg cgcggcagag cgagcgcggc
      301 ccccccggcc agcgccacct tcaggaaatc tcggcgcgac tgcatgctca tctccaaaag
      361 acctcaacgt cgaggttcgc ccgttcagtc ctgcctcctg cggcccgggc ctaggtacgc
      421 agaattacgt aggcgtttcc ggtcgcttca cgagagcacg ccccccatga tctacatgcc
      481 accgttttcg agcccgaggg gatcgtttgc cgccgcgcat cagcctgtgc ttgctcgcct
      541 gcgcgaccgc ggctgcattg cttccggcct ctcccgcaca cgccgcgctg acattcgagg
      601 tcgaggtcgg ccggttcttc gacgaggccg accacaccgc cgagtcgatg cgcttctacc
      661 cctccgcgat cgagatcgcc ccgggtgaca cgctccactt cacgaccgag agcttccacg
      721 gcgtgacgct gctgcccgcc ggggtcgagg ccgggccgtg ggcgggcgag cacgcgaccg
      781 ccggcgggac ctggtctgcg ttcgtggccg accccgacga gggccggcgc ggcgccaaga
      841 tgaacctgcg ggtcgcgtcg ccgtcggatc cctgcggatg gcccggccag tcgccgtgcg
      901 agttcgacgg acgcggcgac gagatggtcg acccgctcaa ctccggcgtc gcgctgttcc
      961 cttccgagtc gggcgtcgag acccgtgagc tgagcttcag cgtgacgatc acggccgacc
     1021 ccggctcgac catcttcgcc gtcgacccgc tccatccggc gatgacaatg cggatcgacg
     1081 tcgtcggctc gttcgcggag cggtccgacc tcggcgagct gcaggccgcg tcggccgcgc
     1141 agttcgccgc cgacgcggac cgtgccgacg cgctcgaccg gacctactcg cgcaagcgcg
     1201 tgaagaagac cgtgcgcggc aagaccgtgt ggcaggcgtg ggccggggtc gaggagcagg
     1261 gcatctcgct ccgccgcttc tatccgagaa agctcaccat cgctcccggc aaccgcgtga
     1321 agtgggtctt ctccaaggac ctctacgagg cacacgcggt gacgttcccg cggacccgcg
     1381 ctcaggcgat ggcgcgggct ttccccgaga tcgcgtgcga cctcgacggc gacgagggca
     1441 ccggggacga cttcgcgccg acctctgccg agttcccgtt ctgttcgagc ggcgagctcg
     1501 aggtcgacgt ccccgcgtcc ttcgtcggcc ccgcgggcaa cggatcgttc cgggggggcc
     1561 gtgacttcga gacgtccggt gcgcgcggcg cctcgttcgc gacgaccaag cgcgcctacc
     1621 agctcacctt cccgaagaag tcgccgaggg ccgggttcgc ctacgcgtgc ccgatccacg
     1681 aggctgcgca cgcgcccatg aacgcggccg tggtggtgaa gcggtgagcc cgcgtctgaa
     1741 gctgtcgctg ctcgccgtcg ccgcggtcgc cgccctggcg gggtgcgagc tcgagaagga
     1801 cccctcggcc acgcctccgc ccgcgccgac acccccgccc gacgtcacga gcccgcaggg
     1861 ggcgtcgaac ccgtcgccgg accggctgat cttctcgtcg ggccgcgacg agcccgacct
     1921 cgagctctac agctcgttcc cggacggttc cgctcgcaag cgcctcacgc ggaacaccga
     1981 gggcgagtcg ggcgtcgtcg cctacccgaa caccgggctc gtctactacg tctgcggccg
     2041 cgcggagtcg atctgcgtcg cgacgacctc ggggaccggc accgccggcg tcttgtcgag
     2101 ccagcgtctc ggcttcccga tcatcgagga ccccgcgatc tctcccgacg ggtcgcagat
     2161 ggccctaacg gcggtgaaga tctcgccgga cgggagcagc acgaactacg acatctacat
     2221 ctacgacttc gcgaccgagg agttcgagaa cttcgccgtc ggtcccgccc tcgaccagat
     2281 gcctgcgtgg gtctcggacg acacgatcgt gtggtcccgg ttccgcaacg gcgactggga
     2341 gctcgtctcg ttccgcctgg acggcccccc cggcagcgcg ccggccgtgc tgacgaacaa
     2401 cgacgtcgac gacctcgggg tggacgtatc ccgcgacggg acgaagctcg cgtggatcgg
     2461 gacgcgcagg gacgcgccga acgccggcga gctgttcacg atgccggtca cgggggggac
     2521 cccgacgtcg ctcgagcccg tgcggctcga gcgcggcttc atcggcgggg atccggacgt
     2581 cgcgtggtcg cccgacggga ccgagatcgc tttcgccggc ctcgcccccg gagaggacga
     2641 cgtcgagatc ttcacgatcc cggccgccga cggccccgtc accaacgtca cgcgcaacga
     2701 cgtctacgac gtcgaccccg agtgggcgac gttgcctccg tcgctgtcgg tcgcgtcgcc
     2761 gctgctcttc accgaaggag ggccgggcgg cgtggtcttc gagatcctgc tcgacgcgcc
     2821 gcagggcgaa gtggtcaggg tcgactacac gacggtcccg gggaccgcga cggccgccga
     2881 cttcacgccc cgcagcggca ccgcggtctt ccagcccggc gagacgaggg tcgcggtgac
     2941 ggtcccggtg acggacgaca cccagatcga gccggccgag acgttctcgc tccggctctc
     3001 caactcttcg gccggaacgc tgaccgcgaa cgaccaggcg accgctcgga tcatggacga
     3061 cgaggtcgag ccgacgccga ctcccccgcc gaccgcgact ccgacgcccg gtcccacttc
     3121 gagccccggc ccggtcaccg acggacggat cgcgttcgcg agcacccgca acggcccgca
     3181 gcagatcttc acgatggcgt ccgacggcac cgacgtgaga cacgtctctc ccgaccagtc
     3241 gtcttcagag ctcggctcgc cgaactggtc gccggacgcg acgcggctga tccacaccgc
     3301 gatgaacaac tccggtggcg cgccgcagcc cgagatcgtc gaccgcccgt cggacgggag
     3361 cgggagcttc cggatcctgc aagtgggccc gtcggtcgac tcggaccccg tctacctgcc
     3421 gggcgacccg ctgggcggct acgtcttcac gagcgaccgc gacggcgact tcgagatcta
     3481 ctacgcgacc ccgtcgggcg ggttcacgaa gaagctgacg gacaacagcg ccaacgacgg
     3541 ccaccccagc ttctcggcgg cctccaacgg catggtgcgg atggtgttcc acagcaacgc
     3601 cgacggtgac ttcgacgtct acaggctcga cgtgtcgacg tccggtgacc cgtcggggtc
     3661 gccggtgaac ctcacgcccg agggcgcggg ccaggccccg cacgacgagc tcgcgccgga
     3721 cttcgcgctg ggtcgcaaca agatcacgta ccacggcaac gagcacggcg acttcgacgt
     3781 ctacgcgctc gacctcggga cgatgcagga gtcgcacctc acgaccgacg tcgcgaacga
     3841 gacgcacccg gctttttcgc cgacggggaa cttcatcgca ttccagaagg acctcggcgg
     3901 tggcaacttc gagatcttcg tgatgtctgc gtccgggggg gccgggacga acgtgacgaa
     3961 ctcgccgggc gccgacctca cgccggagtg gggcacgggg cgttagctcc taccgtccgg
     4021 cccccgtgag ggcccggacg gtcgcgaaga ggtccctcag caacgcctcg acgtcgatct
     4081 gcgccccgct cgcgtgccac tgctcgcgga cgagctccga gacgaccagg ccgatgtccg
     4141 ccgcgagccg gtgctcggct tccaccgggc ccccgtcgcg gtcggcgagg gcctgcgcga
     4201 cgagcgcgat ctgctcctgc cggtgctcgg cccggctctt gcgcagcgcg gggttgcgct
     4261 cgaacagctc ttcgagctcg agcgactcct gcgcgagccc cgagctctgc gactcccgcg
     4321 ccagcgcgag cagcgcaccc tcgaacgcct cgaacgggtt ctccttccgg ggtcgctcgg
     4381 ctatccagcg gcggaagacc tcgcccagct cgcggccccg gtcgaagacg accgactcct
     4441 tcgtcgggta gtagcggaag aacgtcgtgc gcgacacgcc ggcctcggcg gcgatctcgt
     4501 ccacggtcgt gcgctcgaac cccttctcgc ggaacaagcg cagggcggcc gtccgcagcg
     4561 tctcccgcgt ccgggtcttc ttgttgtccc gcagagacat ccgccttcct cctcccaagt
     4621 gggactgaag ccggtcctgg tcccccctca cgtctgcggt gtcaaatatg ggactcagta
     4681 tcactagtta aggggatatg gaccgtgggt ttcttgacgt ggaaggaggc cgtcgtcgcg
     4741 gccgtcctcc tcgcgctcct gatcctctac ctcttcctcc gcgaccgctg aggggtcact
     4801 ggacctccag ggtgcccgtc atcgtgtccg ggtggtactc gcacacgaac tcgtagctgc
     4861 cggcctcgac gtcgcccagg tccacggtca cgctcgcgcc gccctcgacg tcctcgtcca
     4921 caccggcgtc ctcgatcgtc aggttgtgct tcgcctcgtc ctcgttcacg agctcgatcg
     4981 acgagccggc cgacgccgtc agcgagccgg gctcccatgc gaagtccgcc gcggtcagcg
     5041 tgatcgagcc gccgtttccg ccgttcccgc ccgcggccgc gcctccgtcg tctccgccgt
     5101 cgtcgccacc acacgcggcc cccagcaggg ccacgaacgc gacggcaccg atcaacgacc
     5161 ttctcttcga catggcgttc cctttcactc gaccttcacc ttgcctttca tctcggggtg
     5221 gatggcgcac tggtatcggt aggagccggg ctcgtcgaac cggtgcgaga acgactcgcc
     5281 ggttccgagc gcgtcggagc cgaagtcccc gtccggcgac gtcacggtgt ggtctgcggg
     5341 gtcggcgttg cgccacgtga cctcggtccc ggccggcacc acggccgtcg ccggctcgaa
     5401 ggcgaagtcc gctatcgcta tccggccggg ccgcgcctcg tcctcggcct tgacgagagg
     5461 cgcgtccgcg cgcacctcga gcacgccctc catgcccatg tcgtcgtgtc cgtcgaccag
     5521 acactcgatc tcgtagaggc ccggcggcag cgtgagcgtc aggcgcgtcg actcgccggg
     5581 acgcaccacc tcgctctcgg ccttgaagag gtcgcccgat ccgggcccgc tgctgtcgcc
     5641 ctcgagctcc atctcgtagc cgtgccccat cgtcccgcgg ttgtggacga cgaacgtgac
     5701 cctgccggga cggatcgcgg gggcctccgg cgtcacggcc cactcgccga gcacgacctc
     5761 gaacccatcc gcacgaggat ccacgcgctt cggcggcttc ggccgggcgg gctcggccgc
     5821 cgcctggcgc cggacgcgct ggggttccgg ctgcgagctc tgcgcgcccg gaggcgcggg
     5881 atcgcacgcg gccaggccga gcaacgcaac gactacgagc acctgcggtg tcttctgcac
     5941 gagccgtaaa cggggctggg tgccgcggcg ttacgcatag catcgtccga tgccgataga
     6001 gcacgtcagc ctcgacccgt cccgcgacga gatggaggcg atcgcccgcg ccgcggtcgg
     6061 gtacgcgatc gacttcgtcg aagcgcgttc caccgccccg gccgtcgacg tcgacggcgc
     6121 gtacgagctc gcggagaagc tgcggtcggc cccgccggag gagccggccg agttcggcga
     6181 gctgctcgac gtcatcgccc gcgccgccga cgcggcgttc gacaccgcgg ggcccggcta
     6241 cctcgcgtac atccccggcg gcggtctcta tgcggccgcg gtagccgacc tcgtcgcgtg
     6301 cgtgacgaac cgcttcgtga acctcgcggc ccccgcgccg gcgctggtcc gcctcgaagc
     6361 cgacgtcgcg cgctggctct gtgacctgtt cgagttcccg cccgaggcgc agggcgtgct
     6421 cacgtccggc gggtcgatgg cgaacttctc cgcagtcgtc acggcgcgca cggtctgcct
     6481 gcccgaggac ttcctctccg gcacgatcta cgtcagcgag cacgtgcacc actcgatcgc
     6541 gaagagcgcg cgcctcgcgg gcttcccccc ggccgcgctc cgcccggtcc gcaccgacac
     6601 ctccctgcgc ctggacgtcg acgacctgcg cgagcaggtg gcgcgcgacc gggccgaggg
     6661 ccgcaggccc ttcctcgtcg tgggctccgc cgggaccgtc aacacgggcg cggtcgaccc
     6721 gctcgacgag atcgcggcgt tcgccgacga agagggcctc tggttccacg tcgacggggc
     6781 ctacggcggg ttcttccaga tgaccgagcg cggcaaggag cgcttccgcg gcatcgagcg
     6841 ggcggactcc gtcacgctcg acccccacaa gggcctgttc ctgccgtacg ggaccgggag
     6901 cttgatcgtc cggcgcggcg gtgacctgct cgccgcgcac cgcggtggcg cgtcgtacct
     6961 cccggcgcag agcgagaccg accttcccga cttcgccgac tacacgcccg agctgtcgcg
     7021 cgacaaccgc ggcctcaggg tgtgggtccc gctgcacctg cacggcgtag gcgcgttccg
     7081 ccgggccctc gacgagaagc tcgacctcgc cgcgaaggtc tacgacgcgc tcgtggacgt
     7141 ggacgacctc gacgtgccgt gggagccgca gctctcgatc gtcgcgttca agagccgccg
     7201 cggcaacgag gcgaccgacc gcatcctcca ggacgtcaac gcgagcaagc gcgtctacct
     7261 ctccgggacc gagctggaag gcgagcgcta cctccgcgtc gcggtcctgt cgcaccgcac
     7321 cgacgagtca cgcatcgacg aggcgatcga gatcatctcg aaggccgccg cgaactccta
     7381 acgaaaggac cgaacatgtc cgacctcctg acggcgaacc tgctcgtcgt caaccagaag
     7441 gcgaagctga tcgagatgac caaccagtac gcgatccgcg acgccgacgg caacgagctc
     7501 ggccgcatcg agcaggaggg tcagagcaag ctgaagaagg cggtccgcct cgtcggtggc
     7561 ttcgaccagt tcatgacgca cacgctcggc gtctacgacg ccggcggcaa ccgggtgctc
     7621 cagctgacgc gcccgcgcaa ggtcttcaag tcgcgcctcg aggtcaagga ctccggcggc
     7681 cgcaaggtcg gcgagatcgt ccagaagaac atcttcggca agatcagctt cgacttcgtc
     7741 gacccgaacg gacggtcggt cggccggatc aaggcagaga actggcgcgc ctggaacttc
     7801 gcgatcgtcg acgacaccga ccgcgagatc ggccgcatca cgaagaagtt cgtcggcgcg
     7861 ctcaagggca tgttcacgac ggctgacaac tacgtcgtcg agatcgaccc gagcgtcacc
     7921 ggcgacctga ggctgttcgc gctcggcgcg gccgccggcg tcgacacggc cctgaagcag
     7981 gacgaccgcg gcctgaacat cggcggcatc ggaggctagg ccgctacttc gacttcccct
     8041 tcgccttacc gggcggcgtc ttcggagcgg gctcgggcac gggcgcctcg ggagcgggag
     8101 ccgggtcggg ctcggcgggc tctgcgtggg cgggaggctc ctcgaccggc gcgggcttct
     8161 ccttggcggg cgggggcccg gccgcgtcgc cggtcgggcc cgcaggcttc tccggcttcg
     8221 ccgccgtggc gggcttctcg ggcttcgtgg gcttctcggg cttagcgggt ttctcaggct
     8281 tcaccttctc cggcttgtcg ggcttcgagt gccccttcgc cttgccattc ggagtgccct
     8341 cggcgtcgtg cgcgtgcccg agcgccttgg ccttcttgcc gtgcccgagc gcattgccgc
     8401 cccgacccgc ctgcgacttc ccgttgccgc cttcgcgtgc cttcttgtcg tggccgaagg
     8461 cgttccccgc cgggtccggc gagtcgccgt tcgctgcgtc cggtgccgtc tcgtcggctg
     8521 cgggctggac ggcccgcggc ttgtcgcggc cctcgagccg ctgcgcgacc ggccgctcga
     8581 ccgcggcgac cccggtgaac gcgctcgcag tcatcgtccc ggcgagcgaa acggcgccga
     8641 ccgtgttgaa cacgacgccc cactgcgacg cgagtttccg agcgaacggc gcggcgagcg
     8701 cggcgacgcc cgccagcgcg acggccttgt gcggcgtcgc cgtcaggaac gcggacacg
//
LOCUS       DASQDO010000018         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1104506, whole genome shotgun sequence.
ACCESSION   DASQDO010000018 DASQDO010000000
VERSION     DASQDO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104506"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VEV43_00525"
     CDS             complement(<1..186)
                     /locus_tag="VEV43_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HYP22038.1"
                     /translation="MSRVQLALNVTDLEEAIGFYSKLFATEPAKVRSGYANFAVVDPP
                     LKLVLIEGDTGGTLNHLG"
     gene            269..619
                     /locus_tag="VEV43_00530"
     CDS             269..619
                     /locus_tag="VEV43_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYP22039.1"
                     /translation="MKNLQVFEQCCSPLLQEPIAPDEARQLATAFKVLGDPARLRILS
                     LIAAASAGEMCVCEMVEPLGLSQPTVSHHLKVLHDAGLLRREKRGTWAFYGVVPERLD
                     ALRDSLKVPATSPA"
     gene            complement(603..1061)
                     /locus_tag="VEV43_00535"
     CDS             complement(603..1061)
                     /locus_tag="VEV43_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HYP22040.1"
                     /translation="MTLYELLLFVHVLAAATWVGGAIMLKIQSDRALRTDPDRAAELA
                     LETERVGTRVLMPASIVLLVAGLWMVFEGEWGFEPLWVKLGLAIYVISAVAGAVFLGP
                     LYKQVGVLRAERGPGDAELTARLQRISTLSWIDMGLLVAAIFVMTVKPGT"
     gene            1203..2258
                     /locus_tag="VEV43_00540"
     CDS             1203..2258
                     /locus_tag="VEV43_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HYP22041.1"
                     /translation="MNAELFKDLVSTPGISGREERIRDVVQKHMSELVDETRVDALGN
                     LVGIRHGSGTGSGRVMVCAHMDSIGFLVTHIDDNGFLRISPVGGFDPRTLVMQRVVVC
                     GEERDFVGLLAPSSKPIHLLSGDEAKKPPKIEDLFVDLMLPVDEVKAGVSVGDPVSLV
                     REPLVTDEAVTAPYLDDRLGVYVVLEALRKAASSEAEIYSVISVQEEVGVRGATTAAF
                     EIEPAVGVAVDVTIAGDLPGADRSQQVCALGKGAAIGLMDSGSISDPRLVRSFKRLAE
                     DNSIAYQMEILPRGGTDAGGIQLTRAGVPVITISIPIRYVHTVNEMAYVSDIDATVDL
                     LAAYIENAHQTALDWSR"
BASE COUNT          396 a          801 c          794 g          370 t
ORIGIN      
        1 accgaggtgg ttgagggtcc cgccggtgtc gccctcgatc agcacaagct tgagcggcgg
       61 gtcgacgacg gcgaagttgg cgtagccgga ccgcaccttg gctggctcgg tggcgaagag
      121 cttcgagtag aacccgatgg cctcctccag gtcggtgacg ttgagggcga gctggacccg
      181 ggacatgttg acctcctata tcgacggtct tcaatatcga acgacgtgaa tattgcataa
      241 cccatcgacg gatgtcaata taagagccgt gaagaacctc caggtgttcg agcagtgctg
      301 ctcccccctg ctccaggagc ccatcgcccc cgacgaggcg cggcagctcg cgaccgcatt
      361 caaggtcctc ggcgaccccg cccggctccg gatcctcagc ctgatcgcgg cggcctcggc
      421 gggcgagatg tgcgtgtgcg agatggtcga gccgctcggc ctgtcgcagc cgaccgtgag
      481 ccaccacctc aaggtcctcc acgacgcggg cctgctccgc agggagaagc gcgggacgtg
      541 ggccttctac ggggtggtcc ccgagcggct cgacgccctg cgggactcgt tgaaggtccc
      601 ggctacgtcc ccggcttgac cgtcatcacg aagatcgccg cgacgagcag gcccatgtcg
      661 atccacgaca gcgtcgagat gcgctggagc ctcgccgtca gctcggcgtc gccgggcccc
      721 cgctccgccc ggagcacccc gacctgcttg tagagggggc cgaggaacac cgcaccggcc
      781 acggcggata tgacgtagat cgcgaggccg agcttgaccc agagcggctc gaacccccat
      841 tcgccttcga acaccatcca cagcccggcc acgaggagga cgatcgacgc cggcatcagc
      901 acgcgcgtgc ccacgcgttc ggtctcgagg gcgagctccg cggcccggtc gggatcggtc
      961 cggagtgccc ggtcggactg gatcttgagc atgatggcgc cgccgaccca cgtcgcggcg
     1021 gcgagaacgt gtacgaagag gagaagctcg tagagagtca tgtcgcctcc tggacgatgg
     1081 cgtaggccgt gcattaaaac caattccggc gtcaccggac caggtgttac tacgtttgtt
     1141 tgtatttgta ccgatgggca aggaacattg gagcctgctg ggggacgcaa gaggggagac
     1201 tcgtgaacgc cgagctattc aaggacctgg tctcgacgcc gggcatctcc gggcgcgagg
     1261 aacgcatccg ggacgtcgtc cagaagcaca tgtccgagct cgtcgacgag acccgtgtcg
     1321 acgcgctggg gaacctcgtc ggcatccgcc acggctccgg gaccgggtcg ggccgcgtga
     1381 tggtgtgcgc gcacatggac tcgatcgggt tcctcgtgac gcacatcgac gacaacgggt
     1441 tcctccggat ctcacccgtg ggcggcttcg acccccgcac gctcgtgatg cagcgcgtgg
     1501 tcgtctgcgg cgaggagcgt gacttcgtgg gcctgctcgc cccgtcgtcc aagcccatcc
     1561 atctgctgag cggcgatgaa gccaagaagc ccccgaagat cgaggacctc ttcgtcgacc
     1621 tcatgctccc cgtcgacgag gtcaaggcgg gcgtgagcgt gggcgacccg gtgtcgctcg
     1681 tccgcgagcc gctcgtgacc gacgaggccg tcacggcccc ctatctcgac gaccggctcg
     1741 gcgtgtacgt cgtcctcgag gcgctgcgga aggcggcgag ctcggaggcc gagatctaca
     1801 gcgtgatcag cgtccaggaa gaggtcggcg tgcgcggcgc gacgaccgcg gccttcgaga
     1861 tcgagcccgc cgtcggcgtc gcggtcgacg tcacgatcgc gggggacctg ccgggcgccg
     1921 accgcagcca gcaggtctgc gcgctcggca agggggccgc gatcggcctc atggactccg
     1981 gctcgatctc cgacccgagg ctcgtccgct cgttcaagcg cctggcggag gacaactcga
     2041 tcgcctacca gatggagatc ctcccgcgcg gcggaacgga cgccggcggc atccagctga
     2101 cgcgggccgg cgtcccggtc atcacgatct cgatcccgat ccgttacgtc cacacggtca
     2161 acgagatggc gtacgtgtcc gacatcgacg cgaccgtcga cctgctcgcc gcctacatcg
     2221 agaacgcgca ccagaccgcg ctcgactgga gccgttagcc gccgatcccc ccgatgtcta
     2281 cgcgcgtgcg gcgaggtcct ctcggtggac gtggccccga cgctggcttc ggacaaaacc
     2341 gtcgtttgac cccccggatg g
//
LOCUS       DASQDO010000019         9509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_431488, whole genome shotgun sequence.
ACCESSION   DASQDO010000019 DASQDO010000000
VERSION     DASQDO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431488"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..957
                     /locus_tag="VEV43_00545"
     CDS             169..957
                     /locus_tag="VEV43_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP22042.1"
                     /translation="MAQMSSYPHGTFSWVELSTTDTEAAKTFYSQLFGWDTEDAPVPG
                     GVYTMCKKGDAFVGAIQKLQDEMASQGHPPFWMSYVSVDSVDKTAAQVEELGGKILAP
                     PFDVMDAGRMAVVQDPGKAALAIWEAKDHPGAGVVREEGALSWNELSTNDAGTTRDFY
                     TRLFGWSAETSDMGGRDYTVFSTGDDQVAGMMEISPDMGPMPPSWGVYFDGDPDAIAS
                     RAGELGANVIVPPMDFPGGRFTHVADPQGAVFGVVKSVPQSDAG"
     gene            complement(943..1620)
                     /locus_tag="VEV43_00550"
     CDS             complement(943..1620)
                     /locus_tag="VEV43_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYP22043.1"
                     /translation="MTAEVLGRTELFAGLPPRDLEAIAARGIARTYPKGQILFYQHDP
                     GESLYVILEGRVKAFVTSELGTELNLGIFESPAILGEVALVDGGPRSASAEVVAPAKL
                     LVFTRTTFLSLLGEHPALVDAYLRTLGKLIRRLQDRTEDLVFLDLHGRVAKLLLSFAE
                     DAESGPEGTLLDLKFTQGELAAMVAGSRPTVNQILKTFEGRGYLTIAGRQIVVKDEAA
                     LRRLAGV"
     gene            complement(1624..2172)
                     /locus_tag="VEV43_00555"
     CDS             complement(1624..2172)
                     /locus_tag="VEV43_00555"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HYP22044.1"
                     /translation="MDFDHYTDPGVGVAVNLANSLGWPSGTEHLRTPADVCDLLDAQG
                     IEGYGSVEEPDLVALRKLRPELRAIFEAPDEKTAAARINALLARSGAAPQMTDHDGKW
                     HLHYAAEDAPFVDRITALSAMALATVVVNWGWERLGVCNAEDCADVFVDTSRNKSRRY
                     CAPSCSTRTNVAAFRARHKERA"
     gene            2248..2454
                     /locus_tag="VEV43_00560"
     CDS             2248..2454
                     /locus_tag="VEV43_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22045.1"
                     /translation="MHPVLLGQVAQDHVRSLILEADAGRLAAAALGDRPSLRRRTGLR
                     LISLGERLAPECPQNVIPLRGGRI"
     gene            2454..3386
                     /locus_tag="VEV43_00565"
     CDS             2454..3386
                     /locus_tag="VEV43_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYP22046.1"
                     /translation="MALSVSAAVAARRTAFDANPGPAALLLAMLIWGGTLVVTKSLLA
                     GMGAATLLSVRFLVAFLCLVPFAYRSGFRMGDVFRREFVLFGLTGIVLHNGLETWGLE
                     YTSAGSAALIIGGIPAVTVALSRLFLQEPLPRARALGVALSVAGVVLVTRPPSGEGAL
                     ELVGNLLVFGGVVSWAIYTIQGKRMSVRVPAIVGTTAGIGAALLFLLPAAAVESWARG
                     GIPAFGAADVAGLLYLGLGASAAAYALWNFALEHVDASVAAPYVNLVPIIGLALAVLL
                     GESLTPLDFAGGLTVAAGVWLSTSRRFAPAPAAA"
     gene            3511..4488
                     /locus_tag="VEV43_00570"
     CDS             3511..4488
                     /locus_tag="VEV43_00570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HYP22047.1"
                     /translation="MQRLGLTVGFEGAGLRDAVELGRHAEELGYTDLWTAEIGGADAL
                     TPLGALATTTSTVRLGTGILPVFTRPSALVAMSAAALHDLSGGRFVLGLGTSSHVIVD
                     RWMGGRFTRPLTRLREYVEVIRACLRGEKVEFHGLTTRVDGFRLQHQPADVPIHLAAL
                     GPSACALAGEVADGIVFFLKTADGIREALGWANAVRGERDPLECVIRIPFLVDEEPEI
                     VSVAARRLLLSYAPVDVYARSLAAQGFGAEIAAIREAWAAGDRKRAVELVPQAMLDAL
                     FVMGGPEHAARRLDELRAAGVDTPVLLPMSFADPAERAGRVRRAIEALA"
     gene            4530..5105
                     /locus_tag="VEV43_00575"
     CDS             4530..5105
                     /locus_tag="VEV43_00575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP22048.1"
                     /translation="MPAEEGLALYEAGLSAPAGPLLEIGSYCGKSAVYLGAAARERGT
                     VLYSIDHHRGSEEHQPGEEYHDPALVDAHGRVDTFPEFRRTIEGAGLTDVVVPLVAPS
                     SVVARGWTMPLAFVFVDGGHSQAAADDDFAEWAPHVTAGGLLAIHDVFEDPAEGGHPP
                     YVIYRRAIDSGAFAEVSRAGSLRVLERTGVA"
     gene            complement(5018..6073)
                     /locus_tag="VEV43_00580"
     CDS             complement(5018..6073)
                     /locus_tag="VEV43_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HYP22049.1"
                     /translation="MSPLVFDATADAIERVQLRDGSIPWNRGGHLDPWNMVEAAMGLD
                     AARRHEAARAAYRWLIARQRADGAWAASYRDGLIEDSTLDANFCSYLAAGAWHHYLAT
                     GDVDFLAECWPAVDRSMGFVLRLQLPSGAIAWARDASYRPWRAALLTGSACTYLSLGC
                     ALEAAAILGHERLEWELARSALGEAVATRTGPFEPRDRFSMDWYYPALGGVLGGAAAR
                     AALAERWDEFVIEGRGARCVSDRPWVTTGETCELVLALEAAGAGAEAAALFDWVQHLR
                     CDSGDYWTGATWPEDVVWPRETTTWGAGSVLLAADALDGTSPTSAFFHATPVRSSTRN
                     EPARETSANAPESIARR"
     gene            complement(6054..6782)
                     /locus_tag="VEV43_00585"
     CDS             complement(6054..6782)
                     /locus_tag="VEV43_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006899273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP22050.1"
                     /translation="MLTFDFDRFGVGPGTALLDVGCGMGRHTFEALRRGASVVAADLD
                     DEALSAVAEMAGAMAAEGQVPATASLEVVSADARRLPFDDASFDRVVASETLEHISED
                     FVAMSEIARVLRPGGRAVVTVPRWWPEAVCWALSREYHSNEGGHVRIYKRPQLTRRLA
                     ATGLRVDGVEHAHALHSPYWWIKCAVGPRRDEAFLPRLYHRFLVWDMTAQPRSTRLLE
                     RALNPVLGKSVVVYLDKPVEPARV"
     gene            complement(6776..8014)
                     /locus_tag="VEV43_00590"
     CDS             complement(6776..8014)
                     /locus_tag="VEV43_00590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP22051.1"
                     /translation="MTSPRRLRVALLTYRGHPHVGGQGVYVHYLSRALAELGHSVTVF
                     AGQPYPDLAEGVELAEVPSLDLYRPDDPFRRPAPSELKGGTNLLEYGLMCCGAFPEPL
                     TFSLRVAGLLRSRSRDFDVVHDNQCLGYGLLSLGRAVPVVATVHHPITVDRELALAAA
                     PTRRERARLKRWFGFTRMQGRVARRLPRLLTVSEVSRGDVSRAFGVPEHRIGIVHNGV
                     DAELFRPLPGVPKTPGRIVALVSADLPMKGMTHLVEAVAKLRTERDAELVLAGKGADS
                     PGIRAAARKYGVEDSVRVAGRVEDLELVDLYARAEVAVVPSLYEGFSFPAVQAMSCRV
                     PLVATTGGALPEVAGDAALLVPPGDAGALASAIGRLLDDESLRSRAGAAGRRRVLERF
                     TWRSAAEQTAAEYERALRPC"
     gene            complement(8028..8414)
                     /locus_tag="VEV43_00595"
     CDS             complement(8028..8414)
                     /locus_tag="VEV43_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYP22052.1"
                     /translation="MTLLLLAAGIVVGAFSALLGVGGATLMVPFMTLALDYGQHASEG
                     TALLVVVPTAVAGVIAHRRRGYVDFKGAALLASSGVVGAVAGARLALEVSGESLKLGF
                     GIFLMGVGLRMFVHGLRRDGGRRETA"
     gene            complement(8411..8758)
                     /locus_tag="VEV43_00600"
     CDS             complement(8411..8758)
                     /locus_tag="VEV43_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HYP22053.1"
                     /translation="MVGLIAIGAGAGLASGLLGVGGGTVMVPLLVAFLGFSQHRAHAT
                     SLAAIVPIAAVAGGVFALEGVAEYDTALLLAAGALVGAPLGARAMARLGERSLEAAFG
                     AFLVAVGLAMVFG"
     gene            8826..>9509
                     /locus_tag="VEV43_00605"
     CDS             8826..>9509
                     /locus_tag="VEV43_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HYP22054.1"
                     /translation="MAIKHEIKGTVNQMAVCQLDPGQTMYCEAGKFLWKTANVGLETR
                     FTTPDQEEQNKGKGLLGKALSTAVEVGKRQLAGESLAFQYFTPTGGSGLVSFAGTLPG
                     ELREIALDGTKTWTAEKDAFVAAESTVKFDIQFAGFTTGRRGGEGFVLEKFTGTGSLF
                     IAGAGNFIDLNPADYGGKLQVDTGCVVAFDDGISYGVEHIGKFNAQGMKTAMFGGESF
                     SLATLEGNGT"
BASE COUNT         1423 a         3388 c         3388 g         1310 t
ORIGIN      
        1 cggttccgcc gaggagagcg caggcgacga gaacgcaagc gcacacggca atgcttcgcc
       61 tcaggccgcg ctcgatcgtc atgaatcccc catctcgccg gcgggtcgcc gagtccttga
      121 gggtagcctc gggggccgga cggacgaaca ccaataggag gccccgcaat ggctcagatg
      181 agcagctatc cgcacggcac gttctcgtgg gtcgagctgt cgacgaccga caccgaggcg
      241 gcgaagacct tctactcgca gctgttcggc tgggacaccg aggacgctcc cgtgcccggc
      301 ggcgtctaca cgatgtgcaa gaagggcgac gcgttcgtcg gcgcgatcca gaagctccag
      361 gacgagatgg cgtcgcaggg ccacccgccg ttctggatgt cgtacgtgtc cgtcgacagc
      421 gtcgacaaga ccgccgcgca ggtagaggag ctcggcggca agatcctcgc ccctccgttc
      481 gacgtcatgg acgccggccg catggcggtc gtccaggacc cgggcaaggc cgcgctcgcg
      541 atctgggagg cgaaggacca ccccggcgcc ggagtcgtcc gtgaggaggg cgcgctctcc
      601 tggaacgagc tgtcgacgaa cgacgccggc accacccgcg acttctacac gcgcctgttc
      661 gggtggagcg ccgagacgag cgacatgggc ggccgcgact acacggtctt cagcacgggc
      721 gacgaccagg tcgccgggat gatggagatc tcgccggaca tgggcccgat gccgccgagc
      781 tggggcgtct acttcgacgg ggaccccgac gcgatcgcga gccgtgccgg cgagctcggc
      841 gcgaacgtca tcgtgccgcc gatggacttc cccgggggcc ggttcacgca cgtcgccgac
      901 ccgcagggcg cggtcttcgg cgtcgtcaag agcgtgcctc agtcagacgc cggctagcct
      961 ccgcagcgcg gcctcgtcct tcaccacgat ctgccgtccg gcgatcgtca ggtagccgcg
     1021 gccctcgaag gtcttgagga tctggttcac cgtcgggcga gacccggcga ccatcgccgc
     1081 gagctcacct tgcgtgaact tgaggtcgag caacgtgccc tcggggccgg actcggcgtc
     1141 ctccgcgaac gagagcagca gcttcgcgac gcgcccgtgg aggtcgagga agaccaggtc
     1201 ctcggtgcgg tcctgcagac ggcggatgag cttgccgagc gtccggaggt acgcgtcgac
     1261 gagcgcgggg tgctcgccga gcagcgacag gaacgtcgtg cgcgtgaaca cgagcagctt
     1321 cgccggcgcg acgacctcgg cgctcgccga gcgcggcccc ccgtcgacga gcgcgacctc
     1381 gccgaggatc gccggcgact cgaagatgcc gaggttgagc tcggtaccga gctccgacgt
     1441 cacgaacgcc ttcacgcgcc cctcgaggat cacgtagagg ctctcgccgg ggtcgtgctg
     1501 gtagaagagg atctgaccct tcgggtaggt ccgcgcgatc ccgcgggcgg cgatcgcctc
     1561 gaggtcccgc ggcggcagcc cggcgaacag ctcggtccgc cccaggacct cggcagtcat
     1621 ctgctaagcc cgctccttgt gcctcgcccg gaaggccgcc acgttcgtcc gcgtcgagca
     1681 cgacggcgcg cagtaccgac gcgacttgtt ccgcgaagta tccacgaaca cgtcggcgca
     1741 gtcttcggcg ttgcagacgc cgagccgctc ccacccccag ttcacgacca cggtggcgag
     1801 cgccatcgcc gacagcgcgg tgatccggtc gacgaacggc gcgtcctccg cggcgtaatg
     1861 gaggtgccat ttgccgtcgt ggtccgtcat ctgcggagcg gcgcccgacc gggcgagcaa
     1921 cgcgttgatc cgtgccgccg ccgtcttctc gtcgggggcc tcgaagatcg cccgcagctc
     1981 ggggcggagc ttgcgcagcg ccaccaggtc aggctcctcc accgacccgt agccctcgat
     2041 cccctgcgcg tcgaggaggt cgcagacgtc tgccggagtg cggaggtgct cggtccccga
     2101 gggccatccg agcgagttgg cgaggttcac ggcgacgccc acgcccgggt ccgtgtaatg
     2161 gtcgaaatcc atttgacttc ttacctaccg tccctatatg gtgtaagcgt catagcgagt
     2221 atagaccatt acgaagggag ggcgccagtg cacccggtac tcctcgggca agtcgcccaa
     2281 gaccacgtcc gctcgctcat cctcgaggcc gacgcaggcc gcctcgccgc cgccgccctg
     2341 ggcgaccggc cgagcctgcg tcgccgcacg ggtctccgcc tgatctcgct cggcgagagg
     2401 ctggcccccg agtgtcccca gaacgtgatc ccgctccgag gcggccgcat ctgatggctc
     2461 tctcggtctc ggccgcggtg gccgcccgcc gaacggcgtt cgacgcgaac cccggccccg
     2521 ccgcgctgct cctggcgatg ctgatctggg gcggcacgct cgtcgtgacg aagtcgctgc
     2581 tcgccggcat gggcgcggcg accctgctct cggtccggtt cctcgtcgcg ttcctctgcc
     2641 tcgtcccgtt cgcctacagg agcggcttcc gcatgggtga cgtcttccgc cgggagttcg
     2701 tgctgttcgg cctgaccggc atcgtcttgc acaacggtct cgagacgtgg gggctcgagt
     2761 acacgtcggc ggggagcgcc gctctgatca tcggcgggat ccccgcggtc accgtcgccc
     2821 tctcccggct gttcctgcag gagcctttgc cgcgggcccg cgccctcggc gtcgcgctct
     2881 cggtcgcagg cgtcgtgctc gtaacgcggc ccccgtccgg tgaaggcgcg ctcgagctcg
     2941 tcggcaacct gctcgtgttc ggcggcgtcg tgtcgtgggc gatctacacg atccagggca
     3001 agaggatgtc cgtgcgtgtc ccggcgatcg tcgggacgac cgcggggatc ggcgcggccc
     3061 tgctgttcct cctcccggca gcggccgtcg agagctgggc tcgtggaggg ataccggcgt
     3121 tcggcgcggc agacgtggcc ggcctgctgt atctcggcct gggagcttcg gcggcggcgt
     3181 acgcactgtg gaacttcgcg ctcgagcacg tggacgcctc ggtcgcggcg ccctacgtga
     3241 acctggtgcc gatcatcggc ctggccctgg cggtcctgct gggcgagtcg ctgacgccgc
     3301 tcgacttcgc cggcggactg acggtggcgg caggcgtgtg gctgagcacg agccgccgct
     3361 tcgccccggc ccccgccgcc gcataacccc ttcttccgga gtctctggtc gcggtggtac
     3421 gtgtggtgcg tgtggttggg ttcgcaacct cgccaaccac ggcaaccacg cgcaccacgg
     3481 cccccgggag aggtggccta gcatcgccgg atgcaacggc tcgggttgac cgtcgggttc
     3541 gagggggccg ggctccgcga cgccgtcgag ctcgggcggc atgccgagga gctcggctac
     3601 acggacctct ggaccgcaga gatcgggggt gccgacgccc tcactcccct gggcgcgctc
     3661 gcgacgacga cgtcgacggt gcggctcggg accgggatcc tcccggtctt cacccgtccc
     3721 tcggcgctgg tcgcgatgag cgctgccgcg ctccacgacc tgagcggggg ccggttcgtg
     3781 ctcgggctcg gcacctcgtc gcacgtgatc gtcgaccggt ggatgggggg ccggttcacg
     3841 cggccgctca cgaggctgcg cgagtacgtc gaggtcatcc gggcgtgcct ccgcggcgag
     3901 aaggtcgagt tccacgggct gaccacacgc gtggacggct tccggctgca gcaccagccc
     3961 gccgacgtgc cgattcacct ggccgcgctg gggccgagcg cctgcgcgct cgcgggtgag
     4021 gtcgccgacg ggatcgtctt cttcctcaag accgcggacg ggataagaga ggcgctgggc
     4081 tgggcgaacg ccgtgcgcgg cgagcgcgac cccctcgagt gcgtgatccg catcccgttc
     4141 ctcgtcgacg aggagccgga gatcgtctcg gtcgcggcgc ggcggctcct gctgtcgtac
     4201 gcgccggtcg acgtgtacgc gcgctcgctg gcggcgcagg gcttcggcgc ggagatcgcc
     4261 gcgatccgcg aggcgtgggc cgcgggcgac cgcaagcgcg cggtcgagct cgtgccgcag
     4321 gcgatgctcg acgcgctgtt cgtgatgggt gggccggagc acgctgctcg acgactggac
     4381 gagctgcgcg ctgcgggagt ggacacgccg gtgctgcttc cgatgtcgtt cgcggatccc
     4441 gccgagcgag cggggcgcgt ccggcgcgcg atcgaagccc tggcgtgaaa cccgagctcc
     4501 gggcggcggc ggaggcggcc gtcgggttca tgcccgccga ggaggggctc gcgctgtacg
     4561 aggccgggct gtccgcaccc gccgggccgc tgctcgagat cgggagctac tgcgggaagt
     4621 ccgcggtcta cctgggggcc gcggcgcgcg agcgcggcac cgtcctctac tcgatcgacc
     4681 accatcgcgg gtccgaggag caccagccgg gcgaggagta tcacgacccc gcgctcgtcg
     4741 acgcacacgg ccgcgtcgac actttccccg agttccgccg aacgatcgag ggcgcgggcc
     4801 tcaccgacgt cgtcgtcccg ctcgtcgctc cgtcgtccgt cgtcgcacgg ggatggacga
     4861 tgccgctcgc gttcgtgttc gtggacggcg gccacagtca ggccgcggcg gacgacgact
     4921 tcgccgagtg ggccccgcac gtgacggccg gcgggctgct cgcgatccac gacgtcttcg
     4981 aggaccccgc cgagggcggg catcccccgt acgtgatcta ccggcgggcg atcgactccg
     5041 gagcgttcgc cgaggtctcg cgggccggct cgttgcgcgt gctcgagcgc accggcgtag
     5101 cgtgaaagaa cgcggacgtc gggctcgtgc cgtcgagcgc gtccgcggct agcagcaccg
     5161 accccgctcc ccacgtcgtc gtctcgcggg gccacacgac gtcttcgggc cacgtcgctc
     5221 cggtccagta gtcgccggag tcacagcgca ggtgctggac ccagtcgaac agcgcggcgg
     5281 cctcggcccc cgcccccgcg gcttcgagcg cgagcacgag ctcgcacgtc tcgccggtcg
     5341 tcacccaggg ccggtccgag acgcagcggg cgccgcggcc ctcgatgacg aactcgtccc
     5401 agcgctcggc gagggcggcc cgtgcggctg cgcctccgag gacgccgccg agggccgggt
     5461 agtaccaatc cattgagaag cggtccctcg gctcgaaggg gccggtgcgg gtcgccacgg
     5521 cctcgccgag cgcggagcgc gcgagctccc attcgaggcg ctcgtggccg aggatcgcag
     5581 cggcttcgag cgcgcagccg aggctcaaat aggtacaggc cgagccggtg aggagggccg
     5641 cgcgccacgg gcggtacgac gcgtcgcgag cccatgcgat cgcgccggag ggcagctgca
     5701 gcctgagcac gaagcccatg gagcggtcga ccgcgggcca gcactccgcg aggaagtcca
     5761 cgtccccggt cgcgaggtaa tggtgccatg cccccgcggc cagatacgag cagaagttcg
     5821 cgtcgagcgt ggagtcctcg atcaggccgt cgcggtacga cgcggcccac gcaccgtccg
     5881 cgcgctgccg cgctatcagc cagcgatacg cggcgcgggc ggcctcgtgg cgccgtgcgg
     5941 cgtcgagccc catggcagcc tcgaccatgt tccaggggtc gaggtgaccg ccccggttcc
     6001 acgggatcga gccgtcgcgg agctgcacgc gctcgatcgc gtcggcggtg gcgtcaaaca
     6061 cgagcgggct caaccggctt gtcgaggtag acgacgacgc tcttgccgag cacggggttc
     6121 agcgcgcgct ccagcaagcg cgtcgaacgc ggttgcgcgg tcatgtccca gaccaggaag
     6181 cggtggtaga gccgagggag gaacgcctcg tcgcgtctcg gccccaccgc gcacttgatc
     6241 caccagtacg gcgaatggag cgcatgcgcg tgctcgacgc cgtcgacgcg caggccggtc
     6301 gcggcgaggc ggcgcgtgag ctgcggacgc ttgtagatgc ggacgtgacc gccttcgttc
     6361 gagtggtact cgcgcgacag cgcccagcag actgcctcgg gccaccagcg cgggacggtg
     6421 accaccgcgc ggcccccggg ccgcagcacc ctcgcgatct ccgacatcgc tacgaagtcc
     6481 tcggagatgt gctcgagcgt ctccgacgcg accacgcgat cgaacgacgc gtcgtcgaag
     6541 ggcagacggc gcgcatccgc ggagacgacc tcgaggctcg cggttgcggg aacctgtccc
     6601 tcggccgcca tcgcgccggc catctcggcg acggcggaca gcgcctcgtc gtcgagatcg
     6661 gctgctacga ccgacgcgcc gcggcgcaac gcctcgaacg tgtggcgccc catcccgcag
     6721 ccgacgtcga gcagagcggt gcccggcccg acgccgaacc ggtcgaagtc gaacgtcagc
     6781 acggtcgcag agcccgctcg tattcggccg cagtctgctc cgcggcggaa cgccacgtga
     6841 accgttcgag gacgcgccgt cttcccgcgg cgccggcgcg cgaccgcagc gactcgtcgt
     6901 cgagcagccg gccgatcgca gaggcgagcg cgcccgcgtc tcccgggggc acgagcaaag
     6961 cggcgtcgcc ggccacctcg ggcagagcgc ctccggtcgt cgcaacgagc ggaacgcggc
     7021 acgacatcgc ctgaacggcc gggaacgaga acccctcgta gagcgacggc acgaccgcga
     7081 cctcggcccg cgcgtagagg tcgacgagct cgaggtcctc gacgcgtccc gcgacccgga
     7141 cggagtcttc gacgccgtac ttgcgcgccg cggccctgat gcccggcgag tcggccccct
     7201 tgcccgcgag gacgagctcg gcgtcgcgct cggtacggag cttcgcgacc gcttcgacga
     7261 gatgcgtcat ccccttcatc gggaggtcgg cgctgacgag agccacgatg cggccggggg
     7321 tcttcgggac ccccgggagc ggccggaaca gctcggcgtc gacgccgttg tggacgatcc
     7381 cgatccggtg ctcggggacg ccgaacgcgc gggagacgtc accgcgggaa acctcggaca
     7441 ccgtgagcag cctcggcagc cgccgggcga cgcggccctg catccgtgtg aacccgaacc
     7501 aacgcttcag gcgcgctcgc tcgcggcgcg tgggggccgc ggcgagggcg agctcgcggt
     7561 cgacggtgat cgggtggtgc acggtggcga cgaccggcac ggcgcggccc agcgagagca
     7621 gcccgtaacc gaggcactgg ttgtcgtgga cgacgtcgaa gtcacggctc cgggaccgca
     7681 gcaagcccgc gacgcgcagg ctgaacgtca gcggttcggg aaaagccccg cagcacatca
     7741 gcccgtactc cagcaggttc gtcccgccct tcagctccga aggagcgggt cggcggaacg
     7801 ggtcgtccgg acgatagagg tcgagactgg ggacctcggc gagctcgacg ccttctgcca
     7861 ggtccgggta cggctgcccg gcgaacacgg tgacactgtg cccgagctcg gccagcgcgc
     7921 ggctgagata gtggacgtag acgccctggc ccccgacgtg agggtgcccg cgataggtca
     7981 gaagggcgac gcgcagccgc cgtggggaag tcacgaggcg gccaagctta cgcagtctcc
     8041 cgccgccccc catccctgcg caggccgtgc acgaacatcc gcaacccgac ccccatcagg
     8101 aagatcccga agcccagctt cagcgactcg cccgagacct cgagcgcgag cctggcgcca
     8161 gcgaccgcgc ccacgactcc gctcgatgcg agcagcgcgg cccccttgaa gtcgacgtag
     8221 ccgcgcctcc ggtgagcgat cacaccggcg accgcggtcg gcacgacgac gagcagggcc
     8281 gtgccctccg acgcgtgctg gccgtagtcg agcgccagcg tcatgaacgg gaccatcaac
     8341 gtggcaccgc ccacaccgag cagcgcggag aacgccccga cgacgatccc cgccgcgagg
     8401 agaaggagcg tcacccgaag accatcgcga ggccgaccgc gacgaggaag gcgccgaacg
     8461 cggcctcgag cgaccgctcc ccgagcctcg ccatggctcg cgcgccgagc ggcgcgccca
     8521 cgagcgcccc ggccgcgagg agcagcgcgg tgtcgtactc ggccacgccc tcgagggcga
     8581 acactccccc ggccacggcg gctatcggca cgatcgcggc gagcgacgtc gcgtgcgcgc
     8641 ggtgttgcga gaaacccagg aacgcgacca gcaaagggac catgacggtc ccgcccccga
     8701 ccccgaggag cccgctcgcg agacccgcac cggctccgat tgcaatcagc ccaaccacct
     8761 tgaccgcatc ttctcaggct ctctagcgtg ggaagcgact cagctcatcc ccgaaaggcg
     8821 acgtcgtggc gatcaagcac gagatcaagg gcacggtgaa ccagatggcc gtgtgccagc
     8881 tggaccccgg ccagacgatg tactgcgaag ccggcaagtt cctgtggaag accgcgaacg
     8941 tcggcctcga gacccggttc acgacgcccg accaggaaga gcagaacaaa ggcaagggcc
     9001 tcctcgggaa ggcgctgtcg accgcggtcg aggtcggcaa gcgccagctc gccggcgagt
     9061 cgctcgcgtt ccagtacttc acgcccaccg gtgggtccgg tctcgtctcc ttcgcaggga
     9121 cgcttccggg cgagctgcgc gagatcgcgc tcgacgggac gaagacgtgg accgccgaga
     9181 aggacgcatt cgtcgcggcg gagagcacgg tgaagttcga catccagttc gccggcttca
     9241 cgaccgggcg acgcggcggc gagggcttcg tgctcgagaa gttcaccggg acggggtcgc
     9301 tgttcatcgc gggggccggg aacttcatcg acctcaaccc cgccgactac ggcgggaagc
     9361 tccaggtcga cacgggctgc gtcgtggcgt tcgacgacgg catcagctac ggcgtcgagc
     9421 acatcggcaa gttcaacgcg cagggcatga agaccgcgat gttcggcggc gagagcttct
     9481 cgctcgccac gctcgagggc aacgggacc
//
LOCUS       DASQDO010000020         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_921052, whole genome shotgun sequence.
ACCESSION   DASQDO010000020 DASQDO010000000
VERSION     DASQDO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921052"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VEV43_00610"
     CDS             <1..709
                     /locus_tag="VEV43_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HYP22055.1"
                     /translation="LTLRDDHVECALRLDDVRDLAAAVRRCRSLLDLDAGPVAISAHL
                     ARDRSLRRLVRHSPGRRLPGTTDPFELAVRAVLGQQVSVAGARTLAGRLVTARGKPLT
                     APAGGVTHLFPSPDALADADLSEVGMPEARRRALRSLAAAVADGRVVLDAGAERAEAV
                     AQLQALPGIGPWTASYVAMRALGDPDVFLAGDLGVRKAAAGLGLPDAPRDLAAHARRW
                     SPWRSYAVMHLWASLTR"
     gene            720..929
                     /locus_tag="VEV43_00615"
     CDS             720..929
                     /locus_tag="VEV43_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HYP22056.1"
                     /translation="MCRSIKTLRGPEGATDDEIEAAALQFVRKISGYRQPSRANREVF
                     DKAVQEVAEAARELLDDLVTPARAG"
     gene            991..1353
                     /locus_tag="VEV43_00620"
     CDS             991..1353
                     /locus_tag="VEV43_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HYP22057.1"
                     /translation="MASLLVHITHGPEAPSQVALGLLVARTALAEGHDVTVFLAADGV
                     QMIRDRVLDATQGVGTGSAREHFDAIASSGGRFYVSKMSSGARDVTEADLEGKPAQFA
                     TPNDLVRLTLESDRALTY"
     gene            complement(1357..1551)
                     /locus_tag="VEV43_00625"
     CDS             complement(1357..1551)
                     /locus_tag="VEV43_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22058.1"
                     /translation="MARLRDQQRELETILDDLAYELTGARTAGVEQPEILRAALRRAR
                     DLLADADGLAASIGAERSRE"
BASE COUNT          210 a          602 c          631 g          235 t
ORIGIN      
        1 gctgaccctg cgcgacgacc acgtcgagtg cgcgctgcgg ctcgacgacg tgcgcgacct
       61 cgccgcggcg gttcgacggt gccggtcgct gctcgacctc gacgcgggcc cggtcgcgat
      121 ctccgcgcac ctggcgcgcg atcgctcgtt gcggcggttg gtccgccact cccccgggcg
      181 aaggcttccc gggacgaccg acccgttcga gctcgcggtc cgggcggtgc tcggacagca
      241 ggtgagcgtc gccggcgcgc gcacgctcgc gggccgtctc gtcacggccc gcggcaagcc
      301 cctgacggct cccgcgggtg gcgtgaccca tctgttcccg tcgccggacg ccctcgcgga
      361 cgccgacctc tcggaggtcg ggatgcccga ggcacgccgg cgcgcgctgc gttcgctcgc
      421 cgccgcggtg gccgacggcc gggtcgtgct cgacgcgggg gcggaacgcg cggaggccgt
      481 cgcgcagctc caggcccttc ccgggatagg gccgtggacc gcgtcgtacg tcgcgatgcg
      541 tgcgctgggt gaccccgacg tgttcctcgc cggggacctc ggggtgcgca aggcggcggc
      601 cgggctggga cttcccgacg cgccccggga cctcgctgca cacgctcggc ggtggagccc
      661 gtggcgttcc tacgccgtga tgcacctctg ggcaagcttg acccgctagg ctcgccggca
      721 tgtgcaggag catcaagacc cttcgggggc cggagggcgc gacggacgat gagatcgagg
      781 ccgccgcgct ccagttcgtc aggaagatca gcgggtaccg ccagccgtcg cgggcgaacc
      841 gcgaggtgtt cgacaaggca gtgcaagagg tcgccgaggc ggcccgcgag ctgctcgacg
      901 acctcgtgac ccccgcacgc gccggctaga ccggtcccgc gccgacatcc cggcgcgtcg
      961 tcgtcgcgcg cctcgaccag gaggtgacgt atggcttcgc ttctcgtcca catcacgcac
     1021 ggtcccgagg ctccgtcgca ggtcgcgctc ggcctgctcg tcgcgcggac ggcgctggcc
     1081 gagggccacg acgtgacggt gttcctcgcc gccgacggcg tgcagatgat ccgcgaccgc
     1141 gtcctggacg cgacgcaggg cgtcggcacc ggctcagccc gggagcactt cgacgcgatc
     1201 gcgtcgagcg ggggccgctt ctacgtgtcg aagatgtcgt cgggagcgcg tgacgtgacc
     1261 gaggccgacc tggagggcaa gccggcgcag ttcgcgacgc cgaacgacct agtgcggctc
     1321 accctcgaga gcgaccgcgc cctcacgtac tgacgctcat tcccgggagc gctccgcgcc
     1381 gatcgacgcc gcgagcccgt ccgcgtcggc gaggaggtcc cgggcccggc gcagcgccgc
     1441 ccgcaggatc tcgggctgct cgacgcccgc ggtgcgtgcg cccgtgagct cgtaggcgag
     1501 gtcgtcgagg atcgtctcga gctcgcgctg ctggtcccgg agtcgtgcca tgggctgatt
     1561 gatgaccgag ccgggggacc ctgaaacgtg ataaccgttt ggttatagtg agggcatgac
     1621 gggcgggagc gtgatccgga gggcacgcaa gcgggccggg gtctcgcagg ccgagctc
//
LOCUS       DASQDO010000021         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1165872, whole genome shotgun sequence.
ACCESSION   DASQDO010000021 DASQDO010000000
VERSION     DASQDO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165872"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VEV43_00630"
     CDS             complement(<1..1024)
                     /locus_tag="VEV43_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYP22059.1"
                     /translation="MDRRNAAAAAARLTENVEKVIKGNRRAVEAAATALFAGGHLLIE
                     DVPGVGKTMLARSLAASVSGSFKRIQGTPDLLPSDITGTTVFDQQTHHFDFIPGPVFA
                     NVVLVDEVNRATPRAQAALLEAMDEKAVTVDGVRHPLPSPLFVVATQNPLEQHGTFPL
                     PEGQLDRFALSIDMGYVAPATEKEIIVAQLHGHPIEQLGAVLSPDDVMGVQRAVRETF
                     VSQPVLDFALAIVTATRKHPEVALGASPRASVTLIRCAQARALLKGREFVVPDDVKAL
                     ALGTLSHRIVTSGGFGQDAARGRKVMQSILDSTPAPVTDGGHAPPPSSAPSGPSAPAG
                     PPAAPPA"
     gene            1060..1479
                     /locus_tag="VEV43_00635"
     CDS             1060..1479
                     /locus_tag="VEV43_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HYP22060.1"
                     /translation="MGRFEILEHTADVGIRAEAVDLPGVFEQATLGLLDIVGTWAPGR
                     EETEHVDVDIEADDAGALLVDWLGEVLYVGDARDAVVTSLALTEVTSRRAAGRLGIAP
                     RDEAAYEGGTQVKAITYHRLSVEQTGDGWSATVYVDV"
     gene            1490..>2454
                     /locus_tag="VEV43_00640"
     CDS             1490..>2454
                     /locus_tag="VEV43_00640"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYP22061.1"
                     /translation="MEVQRVDEFVWEVPIGWAGGMRVPGRVFASENLIRKAVADSAVD
                     QVANVATLPGIVGASLAMPDIHWGYGFPIGGVAATDVEKGGAVSPGGVGFDIGCGVRL
                     VRSNVTWDQAKPAIRELVTTLGRRVPRGVGGKGRMRLDSAGIEAVLRDGVKYPLSRGV
                     GWEEDAELCEDSGVLPNARPDLVSERAKERGALQLGSLGAGNHFLEVQVVDEIRDAKA
                     AEVMGLFEGGVVVMIHSGSRGLGHQTCTDHLKAIDKLMPNLGIEVPDRQLACVPMDHP
                     NAERYFGAMDAAGNFARANRHLLTDATRASFEQVFGGSARDFEMSV"
BASE COUNT          355 a          809 c          934 g          356 t
ORIGIN      
        1 gagcgggtgg tgctgccggc gggcctgccg gcgccgaggg ccccgacggc gccgaagagg
       61 gaggcggcgc atggcctccg tcggtgacgg gggccggcgt cgagtcgagg atcgactgca
      121 tgaccttgcg gccgcgggcc gcgtcctgcc cgaagccgcc ggaggtgacg atgcggtgcg
      181 agagggtccc gagcgcgagc gccttgacgt cgtcgggcac cacgaactcg cggcccttga
      241 gcaacgccct cgcctgcgcg cagcggatca gcgtgaccga cgcccgcggg ctcgcgccga
      301 gcgcgacctc ggggtgcttg cgcgtcgccg tgacgatcgc cagggcgaag tcgagcacgg
      361 gctgcgacac gaacgtctcg cgcaccgccc tctgcactcc catgacgtcg tcgggagaca
      421 gcaccgcgcc gagctgctcg atcgggtggc cgtggagctg ggcgacgatg atctccttct
      481 cggtcgcggg cgcgacgtag cccatgtcga tcgacagcgc gaagcggtcg agctgtcctt
      541 cggggagcgg gaacgtgccg tgctgctcga gcgggttctg cgtagcgacg acgaacaacg
      601 gcgacggcag cgggtggcgc acgccgtcga cggtcacggc cttctcgtcc atagcctcga
      661 gcaacgcggc ctgcgcccgc ggcgtcgcgc ggttcacctc gtccaccaat acgacgttcg
      721 cgaagacggg gccggggatg aagtcgaagt ggtgcgtctg ctggtcgaag accgtcgtcc
      781 cggtgatgtc cgacggaagg aggtcggggg tcccctggat cctcttgaag ctccccgaca
      841 cggacgcggc gaggctccgg gcgagcatcg tcttgccgac gccgggcacg tcctcgatca
      901 gcaggtggcc tcccgcgaac agagccgtgg cggccgcctc gaccgctcgg cggttcccct
      961 tgatgacctt ctctacgttc tccgtcagac gcgctgcagc cgccgccgcg ttcctgcggt
     1021 ccatcagagg ggcctcactt ctgtgccacg cttgcgggcg tgggacggtt cgagatcctg
     1081 gagcacactg cagatgtcgg cataagagcc gaggcagttg acctcccggg cgtattcgag
     1141 caggccacgc tcggtctgct cgacatcgtc gggacctggg cccccggtcg cgaggagacc
     1201 gagcacgtag acgtcgacat cgaggccgac gacgccggag ccctgctcgt ggactggctc
     1261 ggcgaggtcc tctacgtcgg cgacgcccgc gacgcggtcg tcacctcgct cgcgctgacc
     1321 gaggtcacgt cgcggagggc cgcggggcgc ctgggtatag ctccgcggga cgaggccgcc
     1381 tacgagggcg ggacgcaggt gaaggcgatc acctaccacc gcctgtcggt ggagcagacg
     1441 ggcgacgggt ggtcggcgac ggtctacgtg gacgtctagg agggctgccg tggaggtcca
     1501 gagggtcgac gagttcgtct gggaggtccc gatcgggtgg gccggaggga tgcgcgtgcc
     1561 cggccgggtc ttcgcttcgg agaacctgat ccgcaaagcc gtcgccgaca gcgcggtgga
     1621 ccaggtcgcc aacgtagcga cgctgcccgg gatcgtcggc gcgtcgctcg cgatgccgga
     1681 catccactgg ggctacggct tcccgatcgg aggcgtcgcc gcgaccgacg tcgagaaagg
     1741 cggcgcggtc tcaccgggcg gcgtcgggtt cgacatcggc tgcggcgtgc ggctcgtgcg
     1801 cagcaacgtc acctgggacc aggcgaagcc tgcgatccgc gagctcgtca cgacgctcgg
     1861 gcgtcgggtc ccgcgcggcg tcggcggcaa gggccggatg cggctcgact ccgcgggcat
     1921 cgaggcggtc ctgagagacg gcgtgaagta cccgctgtcg cgcggcgtcg gctgggagga
     1981 ggacgcggag ctgtgcgagg actccggcgt gctcccgaac gcgcggcccg acctcgtgtc
     2041 ggaacgggcg aaggagcgcg gcgctttgca gctcgggagc ctcggggccg gcaaccactt
     2101 cctcgaggtc caggtcgtgg acgagatccg tgacgcgaag gcggcggagg tcatggggct
     2161 gttcgaaggc ggcgtggtcg tgatgatcca ttcggggtcg cgggggctcg gccaccagac
     2221 gtgcaccgac cacctgaagg cgatcgacaa gctgatgccg aatctcggca tcgaggtccc
     2281 cgaccgccag ctggcgtgcg tgccgatgga ccacccgaac gccgagcgct acttcggcgc
     2341 gatggacgcg gcgggcaact tcgcgcgggc gaaccgtcac ctgctgacgg acgcgacgcg
     2401 cgcgtcgttc gagcaggtct tcggcggcag cgcgcgcgac ttcgagatgt cggt
//
LOCUS       DASQDO010000022         7466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_737834, whole genome shotgun sequence.
ACCESSION   DASQDO010000022 DASQDO010000000
VERSION     DASQDO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737834"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..3405)
                     /gene="mfd"
                     /locus_tag="VEV43_00645"
     CDS             complement(169..3405)
                     /gene="mfd"
                     /locus_tag="VEV43_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HYP22062.1"
                     /translation="MASLAPLLDLLPDERVRALLSARGAITVPDPARPFVLASLVRHL
                     DAPVLAIAARGEEAEQLARDVHAFLGREGAEVFPGWEVLPGEPIAPSTDTMGRRLHAL
                     ARLRAGHRFVIVTTAQGATQLVAPPGDVELVEIRPGGTVDLDEMSGRLVDMGYERNYI
                     VERRGEFAIRGGILDVFPPSAERPVRAELWGDEVTSLRQFALASQRSRDDVESVSISP
                     CVERAPEGDGVPVTSFLPKGAPAVVLEPKRVRDRAAEVIGQVEEWAELSGVKEKHYAP
                     LDDALAGLPVALMTSFSEPGAESLQADTWTAAAGRPEVLVDALKSHVRQGFRAVIAAS
                     LEETAQRLAGNLSSSGLSATVVAEAPAPDAPAGVYVVVAELARGFVLPSARLALVAES
                     DITGRRSGAARRRSQAKRRQVSGPFDLAEGDLVVHEVHGIGRYAGMVERELLGVHREY
                     LIIEYLKDDKLYVPSDQVDLVSKYVGGEAPKISRLGSSEWNKAKSRVRRRAREIAQEL
                     VNLYASRLRAKGHSYGPDTPWQRELEEAFPYEETPDQLRAVDEVKDDMERPVPMDRLV
                     CGDVGYGKTEIAVRAAFKAVAAGKQVAVLVPTTILATQHHSTFVERFRSFPVRVEMLS
                     RFLSAAESKKVVADLAEGKVDVVVGTHKLLQPDVKFKDLGLVIVDEEQRFGVDQKEKL
                     KTLRTSVDVLTLTATPIPRTLEMAMSGIRDVSIVDTPPEDRHPVMTYVGEYDEQMIGS
                     AIRRELLRDGQVFYVHHRVDTINHAARVIQELVPDARVGVAHGQMDERTLEQAMLDFG
                     DAKTNVLVCTTIIESGLDIPTCNTLIVERADLLGLSQMYQLRGRVGRAHERAYAYLFW
                     PLDRSITEGAHERLKTISEHTGLGSGFRIAMRDLEIRGAGNMLGAEQHGHIAEIGFDL
                     YVKLMSAAVDEAKGLPWREDGEVRIDLPLNAFIPKSYVADENLRLEAYRRIASAEDGE
                     LEEVKAELLDRYGGPLPEPVEALFEVAALRRLMTGAGITEAATIARNLRIKPIELEDS
                     RQIRLKRLLPEAEWKPATQTLLIPERVVPKTDVVAWVTDLLQQLTSAA"
     gene            complement(3422..4030)
                     /gene="pth"
                     /locus_tag="VEV43_00650"
     CDS             complement(3422..4030)
                     /gene="pth"
                     /locus_tag="VEV43_00650"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYP22063.1"
                     /translation="MRRGSGADVVEDRHVVVGLGNPGERYAQTRHNAGVLAIDVLLDR
                     AGGRLKSHKSGCLVAEVSLGGNRVVLARTTSYMNESGRPVRQLLRWYKQPVENLVVVH
                     DEIDIPFGEVRVKAGGGTAGHNGLGSIVNHLGTKDFARVRVGVGRPPGAKDAAGHVLA
                     GFSSSERKELPFLLVAAADAVEAIAGDGIERAMNEFNTRPKS"
     gene            complement(4040..4735)
                     /locus_tag="VEV43_00655"
     CDS             complement(4040..4735)
                     /locus_tag="VEV43_00655"
                     /inference="COORDINATES: protein motif:HMM:NF004133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HYP22064.1"
                     /translation="MAEVRLQAKVRSDAGKGSARRTRAAGRVPGVVYGRGMDPVAISV
                     DRREFVTALLSDSGMNTLLDLEIDGDTTLALTKELQRDPVRGTLLHADFVKIDRTVEV
                     EVEVPVHIVGEAPGAKEGGVLENPLFTVHVRSLPGDVPESIDADVSTLNLGDSLRVSD
                     LAEGRSFTILDDPETVVASVVAPISEEALASAEADAGIGASEDAEIAAAGAEGATGEE
                     AAEGDAGGAAAAE"
     gene            complement(4899..5240)
                     /locus_tag="VEV43_00660"
     CDS             complement(4899..5240)
                     /locus_tag="VEV43_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22065.1"
                     /translation="MGAADSAALSGWQLYWLGVAGAAIAAILVYILPPLVAAQRTGSV
                     GDWDRTKAILTLALIVLLSLLGGAGPFVLADGQAETAGQAITAGLQAQAVLKALVAAG
                     GEATATPRASA"
     gene            complement(5610..6986)
                     /gene="glmU"
                     /locus_tag="VEV43_00665"
     CDS             complement(5610..6986)
                     /gene="glmU"
                     /locus_tag="VEV43_00665"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HYP22066.1"
                     /translation="MDADAIVLAAGDGKRLKSRLPKVLHHAAGRPLLGHVIAALAPLP
                     LRRRVVVVSPRRDEIVAGLGGLADGIDFVVQEVPRGTGDAVRVGLDGIEEGAVLVTPG
                     DTPLFETETLGALLEAHAASGAGATVLTADMPDPTGYGRVIRSGGDSIDRIVEHRDAS
                     PAELEVREINAGVYAFDVVLLREALGRIGTDNAQGEHYLGDVLGPIAASGRPVMAFKT
                     GHEEVPGVNSRAQLAEAAAALRRRVALGWMSEGVTIVDPATTYIDAGVTIAADATILP
                     FTFLEGTTTIGAGAEVGPQTRVVDSTIEEGASVTFSVVRGSSIGPGASVGPYASLRPG
                     TRLERGARAGTFVETKNTTIGEGSKANHLAYLGDAEIGRGANVGAGSITCNWDGRDKH
                     KTLIEDEAYIGSDTMLVAPVRVGKRAATGAGSVVRGEVPDEALAVGVPARIIEGKGNR
                     MEPKEPRT"
     gene            complement(7011..7082)
                     /locus_tag="VEV43_00670"
     tRNA            complement(7011..7082)
                     /locus_tag="VEV43_00670"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7047..7049),aa:Gln,seq:ttg)
     gene            complement(7103..>7466)
                     /locus_tag="VEV43_00675"
     CDS             complement(7103..>7466)
                     /locus_tag="VEV43_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYP22067.1"
                     /translation="HSPLLTRDVYAGWEPDTGAEAVGSAEMTLALGGGDIPEVASLLY
                     NDLERPAFALRPELAGRKERLVEAGSLGASLSGSGPTLFGIARDEAHAREIAAGVEDD
                     FDKVVVTSSRPECIERLD"
BASE COUNT         1069 a         2735 c         2533 g         1129 t
ORIGIN      
        1 gcgcggcgag ctgctcgaag ctccggctcg cgacgtagtc gtcgagctgc cgctcgacgg
       61 cctcgatcgg gatcttcctg ccgtacacga cggccgcggc ggggtcgagg aggtcgccgc
      121 acgcaggcgc gacgagcaac gcggccatgc cgagaagcgc cagacgtctc atgcggccga
      181 ggttaactgc tggagtagat cggtcaccca cgcgacgacg tccgtcttgg gcacgacgcg
      241 ctccgggatc agcagcgtct gcgtcgccgg cttccactcg gcctccggca acagccgctt
      301 cagccggatc tgccgcgagt cctcgagctc gatcggcttt atccgcaggt tgcgcgcgat
      361 cgtcgccgcc tcggtgatcc ccgcgccggt catcagccgg cgcagcgcgg cgacctcgaa
      421 cagcgcctcg acgggctcgg gcagcggccc gccgtagcga tcgagcagct ctgcctttac
      481 ctcctcgagc tcgccgtcct ctgccgacgc gatgcgccgg tacgcctcga ggcgaaggtt
      541 ctcgtccgcg acgtagctct tcgggatgaa cgcgttcagc gggaggtcga tgcgcacctc
      601 gccgtcctcc cgccacggca ggcccttggc ctcgtcgacg gcggccgaca tcagcttcac
      661 gtagaggtcg aagccgatct cggcgatgtg tccgtgctgc tcggccccca gcatgttccc
      721 ggcgcccctg atctcgaggt cgcgcatcgc gatgcggaag ccggagccga gcccggtgtg
      781 ctcggagatc gtcttgaggc gttcgtgcgc gccctcggtg atcgagcggt cgaggggcca
      841 gaacagatac gcgtaggcac gctcgtgcgc gcggcccaca cgcccgcgca gctggtacat
      901 ctgcgagagc ccgaggaggt cggcccgctc gacgatcagc gtgttgcacg tcggtatgtc
      961 gagccccgac tcgatgatcg tcgtgcacac gagcacgttc gtcttggcgt cgccgaagtc
     1021 gagcatcgcc tgctcgagcg tgcgctcgtc catctgccca tgggcgacgc cgacgcgcgc
     1081 gtcggggacg agctcctgga tcaccctcgc cgcgtggttg atcgtgtcga cccggtggtg
     1141 gacgtagaag acctgtccgt cacgcaacag ctcgcgccgg atcgcgctgc cgatcatctg
     1201 ctcgtcgtac tcgccgacgt aggtcatcac cggatggcgg tcctcgggcg gggtgtcgac
     1261 gatcgatacg tcgcggatcc ccgacatcgc catctctagc gtccggggga tcggcgtcgc
     1321 ggtcagcgtc aggacgtcga cgctggtgcg cagggtcttc agcttctcct tctggtcgac
     1381 gccgaagcgc tgctcctcgt cgacgatcac gaggccgagg tccttgaact tcacgtcggg
     1441 ctggagcagc ttgtgcgtcc cgacgacgac gtcgaccttg ccctcggcga ggtcggcgac
     1501 gaccttcttc gactcggccg cgctgaggaa ccgcgacagc atctcgacgc gcaccgggaa
     1561 cgaccggaag cgctcgacga acgtcgagtg gtgctgcgtc gcgaggatcg tcgtcggcac
     1621 gagcaccgcc acctgcttgc cggccgcgac cgccttgaac gcggcgcgca cggcgatctc
     1681 ggtcttgccg tagccgacgt cgccgcacac caagcggtcc atcgggacgg gacgctccat
     1741 gtcgtccttg acctcgtcga ccgcgcgcag ctggtcgggc gtctcctcgt acgggaaggc
     1801 ctcctccaac tcgcgttgcc acggggtgtc ggggccgtag gagtgaccct tcgcgcgcag
     1861 ccgcgacgcg tagaggttca cgagctcctg cgcgatctcg cgggcccggc ggcggacccg
     1921 cgacttcgcc ttgttccact ccgacgagcc cagccgcgag atcttcgggg cctcgccgcc
     1981 gacgtacttc gagacgagat cgacctggtc gctcgggacg tagagcttgt cgtccttcag
     2041 gtactcgatg atcaggtact cgcggtggac gccgagcagc tcgcgctcga ccatccccgc
     2101 gtagcggccg atcccgtgga cctcgtggac gacgaggtcg ccttccgcga ggtcgaacgg
     2161 tcccgacacc tgccggcgct tcgcctggct ccggcggcgg gccgcgcccg accggcggcc
     2221 ggtgatgtcg gactcggcga cgagagcaag ccgcgccgag ggcaggacga aaccgcgcgc
     2281 gagctcggcg acgacgacgt agacgcccgc cggcgcgtcg ggggccgggg cctccgcgac
     2341 gacggtcgcc gagaggcccg acgacgacag gttgccggcc agccgctggg cggtctcctc
     2401 cagcgacgcg gctatcacgg cgcggaagcc ctgccgtacg tgcgacttca acgcgtcgac
     2461 gaggacctcc ggacgccccg ccgcggcggt ccaagtgtcc gcctgcaacg actcggcccc
     2521 cggctccgag aagctcgtca tcagcgcgac cgggagcccg gccagcgcgt cgtcgagcgg
     2581 cgcgtagtgc ttctccttca caccgctgag ctcggcccat tcctcgacct ggccaatgac
     2641 ctccgcggcc cggtcgcgca ctcgcttcgg ctcgagcacg accgccggcg cgcccttcgg
     2701 gaggaagctc gtcaccggca cgccgtcgcc ctccggcgcc cgctcgacgc acggcgagat
     2761 cgagacggac tcgacgtcgt cgcgcgaccg ctgcgacgcg agcgcgaact gccgcaacga
     2821 cgtgacctcg tcgccccaca gctccgcacg gaccggccgt tcggccgacg gcgggaagac
     2881 gtcgaggatt ccgccgcgga tcgcgaactc gccgcggcgc tcgacgatgt agttgcgctc
     2941 gtagcccatg tcgacgagcc ggccggacat ctcgtcgagg tcgaccgtcc cgccgggacg
     3001 gatctcaacg agctcgacgt cacccggcgg agcgacgagc tgcgtggctc cctgcgcggt
     3061 cgtcacgatg acgaagcggt gcccggccct cagccgcgcg agcgcgtgca ggcgccgccc
     3121 catcgtgtcg gtggacggcg ctatcggctc gcccgggagc acttcccatc ccgggaagac
     3181 ctcagccccc tcgcggccca ggaacgcgtg tacgtcgcgg gccagctgct cggcctcctc
     3241 accccgcgcc gcgatcgcga gcaccggcgc gtcgagatgg cggaccagcg acgcgagcac
     3301 gaacggccgg gccgggtccg gcaccgtgat ggcgccgcgc gcggacagga gcgcgcggac
     3361 gcgctcgtcg ggaaggaggt ccagaagggg tgccaacgaa gccacggaga cgccgatctt
     3421 ctcaggattt ggggcgtgta ttgaactcgt tcatcgcacg ctcgatcccg tcgccggcga
     3481 tcgcctcgac ggcgtcggcg gccgccacga gcaggaacgg cagctccttg cgctcggacg
     3541 acgagaaccc ggcgaggacg tgtccggccg cgtccttcgc gcccggcgga cggccgacgc
     3601 cgacccgcac ccgcgcgaag tccttcgtgc cgaggtggtt cacgatcgag ccgaggccgt
     3661 tgtgccccgc ggtccccccg ccggccttga cccggacctc gccgaacggg atgtcgatct
     3721 cgtcgtgcac gacgacgagg ttctcgacgg gctgcttgta ccacctcagc agctgccgca
     3781 cggggcgtcc ggactcgttc atgtacgacg tcgtccgcgc gagcacgact cggttcccgc
     3841 cgagcgagac ctcggctacg agacagccgc tcttgtggct cttcaggcgc ccgcccgctc
     3901 gatcgagcag gacgtcgatt gcgagcacgc cggcgttgtg acgcgtctgc gcgtagcgct
     3961 cgcccgggtt cccgagcccg acgacgacgt ggcggtcttc gacgacgtct gcgcccgagc
     4021 cccggcgcag cagacgcaac tactcggcgg ccgcggcccc gcccgcgtcg ccttccgcag
     4081 cctcttcgcc cgtggcgcct tcggcccccg cagcggcgat ctcggcgtcc tcggaagccc
     4141 cgatgcccgc gtcggcctcc gccgaagcca gggcctcctc ggagatcggc gccacgacgc
     4201 tggcgaccac ggtctcgggg tcgtcgagga tcgtgaacga gcggccttcg gccaggtcgg
     4261 agacgcgcag gctgtcgccg aggttcagcg tcgagacgtc ggcgtcgatg gactcgggca
     4321 cgtcacccgg aagcgagcgg acgtgcacgg tgaacagcgg gttctcgagg accccgccct
     4381 ccttcgcacc cggcgcctcg ccgacgatgt gaacgggcac ctcgacctcg acctcgacgg
     4441 tgcggtcgat cttgacgaag tcggcgtgaa gcagcgtgcc cctgaccggg tcgcgctgga
     4501 gctccttcgt gagggccagc gtggtgtcgc cgtcgatctc gaggtcgagg agcgtgttca
     4561 tcccgctgtc ggagagcagc gcggtgacga actcacggcg gtcgaccgag atcgcgaccg
     4621 ggtccatgcc ccgcccgtag acgacgcccg gcacgcgccc ggccgcacgc gtccggcggg
     4681 ccgacccctt cccggcgtcg ctccgcacct tcgcctgcag cctgacctca gccatcgtcc
     4741 atctccttcg tccacggtcg ttccttggcg gatcgtcgag acgatccgtc gttcgctgcc
     4801 gcgccgagaa cggcctggtt cccgggggcg cccggccttc gagggcctgg gcaggagggc
     4861 cattatggct gctcgggggc cgcggctcaa acggagcgtc aagcagaggc tctaggcgta
     4921 gctgtggctt cgccgccggc cgcgacgagc gccttcagaa cagcctgcgc ctggagacct
     4981 gctgtgatcg cttgtcccgc agtttcggct tgcccgtcgg ccaatacaaa cggcccagcg
     5041 ccgccgagca aagagaggag gacgatgagc gcaagggtga ggatcgcctt cgtccgatcc
     5101 caatctccga cgctcccggt ccgctgcgca gcgacaagtg gcggcaatat gtagacgagg
     5161 atcgccgcta tggccgcgcc ggcaacgcca agccagtaaa gctgccagcc cgagagggcg
     5221 gcggagtccg ccgctcccat tacttggacg aggcctggga ccgtttctcg cccgtctccg
     5281 gtcgggtcgc ggcggccttc ccttggccgt tgccctcgta cacgagggac agaccggccg
     5341 tcgacaagat ccggaagatc ttatggagct tggctgcggt catcggcact cctgttcgag
     5401 ggctttctgt caattcggag gacttcgccc ttgactcaac taagtcaatc cataatacgc
     5461 acgagaatag taaagagcta cggccgaggc tcatctcaag cgtccgtagg gtcagaaggc
     5521 tcgtcaacgg cgattcgtcg tctgaaacgg gagcaacaag gcgccgtcgc gaacgatagg
     5581 gagcgtccgt gaccggccta ggcccggggc taggtccgcg gctccttggg ctccatccgg
     5641 ttccccttgc cctcgatgat cctcgccggg acgcctacgg ccagggcctc gtccgggacc
     5701 tcgccccgca cgaccgagcc ggcgcccgtc gcggccctct tgccgacccg gaccggcgcc
     5761 acgagcatcg tgtccgagcc gatgtaggcc tcgtcctcga tcagcgtctt gtgcttgtcc
     5821 cggccgtccc agttgcaggt gatcgagccg gcgccgacgt tggcgccgcg gccgatctcg
     5881 gcgtcgccga ggtaggcgag gtggttcgcc ttgctcccct cgccgatcgt cgtgttcttg
     5941 gtctcgacga aggtcccggc cctcgcgccg cgttcgagcc gggtccccgg ccggagcgac
     6001 gcatacgggc cgaccgaggc gcccgggccg atggacgagc cgcgcacgac cgagaacgtc
     6061 acgctcgcgc cttcctcgat cgtcgagtcc acgacgcgcg tctgggggcc gacctcggcc
     6121 ccggccccga tcgtcgtggt tccttcgagg aacgtgaacg gcaggatcgt cgcgtcggcc
     6181 gcgatcgtca cgcccgcgtc gatgtaggtc gtggcggggt cgacgatcgt cacgccctcg
     6241 gacatccagc cgagagccac ccgtcggcgc agcgccgcgg ccgcctccgc gagctgcgcc
     6301 cgcgagttca cccccgggac ctcctcgtgg ccggtcttga acgccatcac cggccgaccc
     6361 gacgccgcga tcgggccgag gacgtcgccg aggtagtgct cgccctgcgc gttgtcggtg
     6421 ccgatccgcc cgagggcctc gcgcagcaac acgacgtcga aggcgtagac gccggcgttg
     6481 atctcgcgca cctccagctc ggccggggat gcgtcgcggt gctcgacgat gcggtcgatc
     6541 gagtcgccgc cggaccggat cacgcgcccg tagcccgtcg ggtcgggcat gtccgcggtc
     6601 aggaccgtcg ccccggcccc ggaggccgca tgggcttcga gcagcgcgcc gagcgtctcg
     6661 gtctcgaaca gcggggtgtc ccccggcgtg acgaggacgg ccccctcctc gatgccgtcg
     6721 agcccgaccc ggaccgcgtc gccggtcccc cgcgggacct cttgcacgac gaagtcgatc
     6781 ccgtccgcca ggccgccgag cccggcgacg atctcgtcgc gacgcggcga gacgacgacg
     6841 acccgccggc gcagcgggag cggcgcgagg gccgcgatca cgtgcccgag caacggacgg
     6901 cccgcggcgt gatggaggac cttcggcagc cgcgacttca gccgcttgcc gtcacctgcg
     6961 gcgaggacga tcgcgtcggc gtccattgct cctccactcg acgtacgaag ctgccccgcc
     7021 tggattcgaa ccagaactta gggaaccaaa atccctcggg ctgccactta ccccacgggg
     7081 caccggaaag tgccgtctca tgctagtcga ggcgttcgat gcactcgggt cgcgacgacg
     7141 tgacgacgac cttgtcgaag tcgtcctcga cgcccgccgc gatctcgcgc gcgtgcgcct
     7201 cgtcccgcgc tatcccgaac agcgtcggtc ccgagcccga caacgacgcc cctagcgacc
     7261 ctgcctcgac cagccgctcc ttccgcccgg cgagctcggg ccggagcgcg aacgccgggc
     7321 gctcgaggtc gttgtagagg agcgacgcga cctccgggat gtccccgccc ccgagggcga
     7381 gcgtcatctc ggcgctcccg acggcctcgg cccccgtgtc cggctcccac cccgcgtaga
     7441 cgtcacgggt caggagcggg ctgtgc
//
LOCUS       DASQDO010000023        11657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_982856, whole genome shotgun sequence.
ACCESSION   DASQDO010000023 DASQDO010000000
VERSION     DASQDO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982856"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VEV43_00680"
     CDS             <1..254
                     /locus_tag="VEV43_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYP22068.1"
                     /translation="ATWYGPGFYGNTTACGRTLGYSTLGVAHRTLPCGTKVAIMYRGR
                     TLMVEVIDRGPYARSNWDLTRETAERLRFSGTDNIGVAH"
     gene            complement(259..471)
                     /locus_tag="VEV43_00685"
     CDS             complement(259..471)
                     /locus_tag="VEV43_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22069.1"
                     /translation="MAGWGDDPELERLRGLIDDGWEVVDVTEDSSSGAPMDTVRLRKG
                     DQETECTSDHLAFHRYVEGLQEEQSQ"
     gene            complement(505..1533)
                     /locus_tag="VEV43_00690"
     CDS             complement(505..1533)
                     /locus_tag="VEV43_00690"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HYP22070.1"
                     /translation="MTEQQDLLRVEDLKVYFPIRSGLLVDRHVGDVKAVDGVSFSIKR
                     GETLGLVGESGCGKTTVGRTVLRLYDPTDGHIYFDGVDVAGLSGGELRKLRRRMQMIF
                     QDPYSSLNPRQSVGNIVGEPLKIHGSSDDEITDKVRHLIEVVGLPEGAANRYPHEFSG
                     GQRQRIGLARSLAMNPDLIVADEPVSALDVSIQAQIVNLLEELQDEFQLTYLFIAHDL
                     AVVRHISDRIAVMYLGMIMEVAPAEDLYDRPLHPYTKALLSAVPIPDPKVERGRERIL
                     LRGDLPSPANPPPGCRFHTRCPFVQKERCPDEVPELREVGGHTVACHWVEEIESGQIQ
                     PHEAGVPV"
     gene            complement(1530..2537)
                     /locus_tag="VEV43_00695"
     CDS             complement(1530..2537)
                     /locus_tag="VEV43_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP22071.1"
                     /translation="MALLSVRDLEVKFFMRRATINAVNGISFDLDAGETLAIVGESGC
                     GKSVSSLAILGILPKPAGKITGGEVRFADVDLVGLPDSKLREIRGRDIAMIFQDPMTS
                     LNPVLRVGRQITETLRKHLDMGKAEADEEARSLLERVGIPDAASRLRDYPHQFSGGMR
                     QRVMIAMAIACRPKILIADEPTTALDVTIQAQILDLLRHLVEEENMALILITHDLGVV
                     AGICDRTNVMYAGEFIETAPTAELFARPRHPYTLGLLRSVPRLDAARKEKLQPIGGTP
                     PDLRSRPDACSFAPRCAYATQESRERRPALHQIDGPDHFVACWNPVPVEDMPIQGEET
                     A"
     gene            complement(2541..3437)
                     /locus_tag="VEV43_00700"
     CDS             complement(2541..3437)
                     /locus_tag="VEV43_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP22072.1"
                     /translation="MSVAEAEAKTRAVEGQAGLWSDAFDRLKRNPGAVAGSIVVVLFV
                     IVAVFAPLVAPYDPNEQVGSLAANPEGPSGEHWFGLDEQGRDFFSRVVYGSRLSLLTG
                     VVSVAIGLLFGVALGALAGYLGGWVDTVIMRFMDIMLSIPGLLLAIAIVTATGRGLLP
                     IMLAIGIINVPVFARLLRGSILAQREADYVLAARSLGATGPRLLGVHILPNAIAPVNV
                     QATLAMGTAIIEVAALSFLGLGPADPGFPEWGKMVADNSNRLTVSTHLIFFPGFAIVL
                     SVLGINLIGDGLREAIDPKLRA"
     gene            complement(3434..4438)
                     /locus_tag="VEV43_00705"
     CDS             complement(3434..4438)
                     /locus_tag="VEV43_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP22073.1"
                     /translation="MLRYVVRRLVLLVPILIGLSILVFVFVRSLPGGPAIALLGERAT
                     QESVEAIERELGLDKPVHEQYLAYAGNLLSGDLGASIQTRRPVVDELKDRFPATIELG
                     FAAMLFAILAGVPLGYVAAKRYQGFLDQSSLVLSLIGISFPIFFLALILKYVFAVKLG
                     WLPSIGRLDVTRTLEHPTGFYTLDALIAGDFPAFMDAVRHLVLPAIALGTIPLAIVAR
                     ITRAATLDVLSEDYVRTARAKGLAPSVVDRRHVMRNALLPVVTIVGLQTGLLLTGAIL
                     TERVFAWPGLGTMIINAISFRDYAVLQGGLVFFALIIVLVNLLVDVSYAFLNPRIRYR
                     "
     gene            complement(4514..6214)
                     /locus_tag="VEV43_00710"
     CDS             complement(4514..6214)
                     /locus_tag="VEV43_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYP22074.1"
                     /translation="MPKPFRLLALALVLALTLVACGGDDGESEEEGGGDDAGAATATL
                     TFATSADPVVLDGALVSDGESLRAIDQIFEGLVTLAPGGTEVVPNLATDWTTSEDGLD
                     WTFNLRDDVTFHDGEAFNADAVCYNFDRWYNFEGSFQNPAATYYWQTVFGGFKKTDPD
                     SGAPTESNFKSCTVDDEHTVTISLNNPSSSFLAAMALTNFTFGSPKALEEYDANEGRV
                     NAEGIFRATGTYGTEHPIGTGPFMFEEWVRNDHLTMTRNPDYWGEEDATGNIQTLIFQ
                     PIPDNAARLQALETGEIQGYDLVEPQDMETISGNPDLQLLERPAFNVGYIGMNQAQKP
                     LDDIEVRKAIAHAINRQEVVDAFYAGQGEVATQFMPPEVFGYADDVTTYDYDPDKAKQ
                     ILEDAGYTLPVEIDFAYPTDVSRPYMPVPQDNFEAMVADLEECCFKVEKKSAIWSPDY
                     LDNVDNGRYGLYLLGWTGDFGDPDNFVGTFFQTEQPAWGFDNKEIFDCLDAAETEVDQ
                     DARVAKYEECNRLIMDFLPGLPYVHTKPALAFNANVTGYQPSPVSLEPFSIVTVEGGA
                     "
     gene            6445..7431
                     /locus_tag="VEV43_00715"
     CDS             6445..7431
                     /locus_tag="VEV43_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYP22075.1"
                     /translation="MAQSPPPSNGGRDFSEFQVAFDRIVDNMERTIQGKTGTVRLALV
                     TMIAEGHVLLEDIPGVGKTMLAKSLAKSVGCQWQRIQFTPDLLPSDVTGVNVWDRASG
                     EFVFKPGAVFANICLGDEINRASPKTQSALLECMEERQVTVDGTTRVLDMPFMVIATQ
                     NPIEHEGTYPLPESQLDRFMMRLSMGYPNRESELEILETHGRTSLLDQLEPVADANEV
                     NNLIKLAREVHVAPSLRRYIVDLAEATRDHPDVYLGASPRASLFLLKASRALAASRGR
                     DYVVPDDIKDLVVPVLAHRVLLSPEAQMRGSSAEAVLEGLSGRVPIPAKEQA"
     gene            7454..8743
                     /locus_tag="VEV43_00720"
     CDS             7454..8743
                     /locus_tag="VEV43_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYP22076.1"
                     /translation="MLTLRGWAVALTGLALLVVARIFGTGAVEQFGFALVVLPLIALA
                     VVRLGKHDLEVTRRTSPERVRAGSKVRVSLEIRNRGRGPAPLLLLEDRVPSDVAGRAR
                     FALHGVEPKGRRVASYELRPPRRGNYQVGPLTLTSTDPFGLARLESAAAPKASILVHP
                     RVEPLTLPRDLGDRRSLAVSALRQPIGAQGEDFYTLREYNEGDDLRKVHWPATAKRGR
                     YMIRQEETPWHNRATVVFDDRTTAHDGIGDASSFERVIEATASIVDLYQRSGYTYRLA
                     GGVSNGLPASKGTPHFNRALDLLATVRTSPPSALEADPLIARLLEIDALTSGEGTLAV
                     VTGSLTPDAAMAITRCRRRFRQVIVVVFPAHRFGTLTTKARWAGEEEVGEVVKLLGRA
                     GAQAIVLGPEESLLSGWDSLTRSASSAVRSKPGATVR"
     gene            8740..11055
                     /locus_tag="VEV43_00725"
     CDS             8740..11055
                     /locus_tag="VEV43_00725"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HYP22077.1"
                     /translation="MSSEGRARVGLSILLLLTLFSFAQVFAEGDYPGPVLLGMVLASA
                     LAIGARRLGLWTSTTIAGSLAVLFLYLVAIFQARNSFYLLPSPDAVAGLGRSIGNALR
                     HSSVDYAPVPVRTGYVVLVVIGMWVATTIGEIATFRWRRPLLAALPCIGLFCIQMTVG
                     TGTGAPALVIAFLLALLTYWGLEAAHRVRSWGRWVAAFPGTGRDGKEPESVTGALARR
                     MGAGCVAAAIVAPFVVPALGDGLLAWRNETGEGGLGSGGGSGEINPFVSIAPRLIEQT
                     DEVLFTVRSERPEYWRLLSLADFDGTQWDPVPSSDSEVVADGEEFRRQTNSPQSSEPL
                     EHTITFAALDSTRLPTALNPYVVDLEDGGEFTYDGENQELTLDGEAGEGFEYAVVSNV
                     PDLSYDRLRKAEVGFPGQVYADDGHVSPDVDALAADIRTTANGRALSDADYLIALQEY
                     LRGFTYDITPEDAELAASGGSVDFLERFLFDTQTGYCQQFATAFALLARAEGIPTRVA
                     VGFLPGSSSQEEDDTYVVSGLHTHAWPEVYFDGYGWVRFDPTPRLEAPQPRYTREPIE
                     AGGPGTAGGTENGGIGAALGRQDPIEDRRLAGANTGAATGADRPAGTAIGDRRVAEDP
                     VWEKAFNRVALGGALVLVLFLASVPALKTWRISRRYRRARGDDGAAAAAFAHFEDEAA
                     ELASPRDPAESAVAYALRLAGLRKVPKEDATRLASIFEAAEYAPGGIRPDQAGQARAL
                     ARKLRGALWARASWWARAGRLFSPRRLVGRP"
     gene            complement(11038..11556)
                     /locus_tag="VEV43_00730"
     CDS             complement(11038..11556)
                     /locus_tag="VEV43_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYP22078.1"
                     /translation="MEGFTAQQTSRFTGCTAHQLRYWDRIGLVKPSVQATGGRPGVRR
                     LYSFRDLVALKVVKSLLDHGMSLQRVRRAYSYLRKRAALDDHLSEVKLVTDGQSIFKI
                     ARNDGELLDALKEGQLAFFIAIDEIAAGVDGRVAEYLYDREEFVTALRQVEEKLEQDV
                     PEEALRRLRAAN"
BASE COUNT         1741 a         4044 c         4081 g         1791 t
ORIGIN      
        1 tcgcgacctg gtacggcccc gggttctacg ggaacacgac ggcgtgcggc cggacgctcg
       61 gttacagcac gctcggcgtc gcgcaccgga cgctcccctg cgggacgaag gtggcgatca
      121 tgtaccgggg ccggacgctg atggtcgagg tgatcgaccg ggggccgtac gcacggtcga
      181 actgggacct cacgagagag acggccgagc gcctgcgctt ctccgggacg gacaacatcg
      241 gagtagcgca ctaaggagct actgggactg ctcctcttgt agtccctcga cgtagcggtg
      301 gaaggccagg tggtccgagg tgcactcggt ctcctggtcg cccttgcgga gacggaccgt
      361 gtccatgggc gcgcccgagc tcgaatcctc ggtgacgtcg acgacctccc agccgtcgtc
      421 gatcagcccg cgcagtctct cgagctccgg gtcgtctccc caaccggcca ccgtctcctc
      481 ctttagtagg cgtcgggcga gaggttagac cggaacgccg gcctcgtggg gctggatctg
      541 gcccgactcg atctcctcca cccaatggca ggcgaccgtg tggcccccga cctcgcgcag
      601 ctccggcacc tcgtccgggc acctctcctt ctgtacgaac ggacagcgcg tgtggaagcg
      661 gcagccgggc ggtgggttcg ccggcgacgg gaggtcgccg cgcagcagga tgcgttcgcg
      721 cccgcgctcg accttcgggt ccgggatcgg caccgcggac agcagcgcct tcgtgtacgg
      781 gtggagcggc cggtcgtaga ggtcctccgc cggggcgacc tccatgatca ttccgaggta
      841 catcaccgcg atgcggtccg agatgtgccg cacgaccgct aggtcgtgcg cgatgaacag
      901 ataggtgagc tggaactcgt cctgcagctc ctcgaggagg ttcacgatct gtgcctggat
      961 cgagacgtcg agggccgaga ccggctcgtc ggcgacgatc aggtccgggt tcatcgcgag
     1021 cgagcgggcg agcccgatgc gctgacgctg gcccccggag aactcgtggg ggtagcggtt
     1081 cgcggccccc tccgggagtc ccacgacctc gatcaggtgc cgcaccttgt cggtgatctc
     1141 gtcgtcggac gacccgtgga tcttcagcgg ctcgccgacg atgttgccga cgctctggcg
     1201 cgggttcaac gacgagtacg ggtcctggaa gatcatctgc atccggcggc ggagcttgcg
     1261 gagctcgccg cccgacaggc ccgcgacgtc gacgccgtcg aagtagatgt ggccgtcggt
     1321 cgggtcgtag agccggagga ccgtgcggcc gacggtcgtc ttgccgcagc cggactcgcc
     1381 cacgaggccg agggtctcgc cccgcttgat cgagaacgac acgccgtcga cggcctttac
     1441 gtcgccgacg tggcggtcga cgaggaggcc ggaccggatc gggaagtaga ccttgaggtc
     1501 ctcgacccgc agaaggtcct gttgctcggt cacgctgtct cctcgccctg gatcggcatg
     1561 tcctcgaccg ggaccgggtt ccagcacgcc acgaagtggt cgggcccgtc gatctggtgc
     1621 aaggccgggc gcctctcgcg ggactcctgc gtcgcgtatg cgcagcgggg cgcgaacgag
     1681 cacgcgtcgg gccgcgaccg caggtccgga ggcgtccccc cgatgggctg gagcttctcc
     1741 tttcgcgccg cgtcgaggcg cggcaccgac cggagcagcc cgagggtgta ggggtgacgg
     1801 ggccgcgcga acagctcggc cgtcggcgcc gtctcgatga actcgcccgc gtacatcacg
     1861 ttcgtgcggt cgcatatgcc ggcgacgacg ccgaggtcgt gcgtgatgag gatcagcgcc
     1921 atgttctcct cttcgacgag gtgcctcagg aggtcgagga tctgcgcctg gatcgtcacg
     1981 tcgagcgcag tcgtgggctc gtcagcgatc aggatcttcg gccggcacgc gatcgccatc
     2041 gcgatcatca ccctctgccg catgccgccc gagaactggt gcgggtagtc gcgcaaccgg
     2101 ctggcggcgt cggggatgcc gacgcgctcg agcaggctgc gcgcctcctc atcggcctcg
     2161 gccttaccca tgtcgaggtg cttgcgcagg gtctcggtga tctgtctgcc gacgcgcaag
     2221 acggggttca gcgacgtcat cgggtcctgg aagatcatcg cgatgtcgcg gcccctgatc
     2281 tcgcgcagct tcgagtcggg cagcccgacg aggtcgacgt cggcgaagcg gacctcgccc
     2341 cccgtgatct tgcccgccgg cttcggcagg atcccgagga tcgcgagcga cgacacgctc
     2401 ttcccgcagc cggactcgcc gacgatcgcc aaggtctcgc ccgcgtcgag gtcgaacgag
     2461 atcccgttga cggcgttgat cgtggcgcgg cgcatgaaga acttcacctc gaggtcgcgc
     2521 acggagagca gcgccatcgc tcaggccctc agcttggggt cgatggcctc gcgcaggccg
     2581 tcaccgatga ggttgatgcc gagcacgctc agcacgatcg cgaagcccgg gaagaagatg
     2641 agatgcgtcg agaccgtcag ccggttcgag ttgtcggcga ccatcttgcc ccactcgggg
     2701 aagccggggt cggcggggcc gagcccgagg aagctcagtg ccgcgacctc gatgatcgcc
     2761 gttcccatcg cgagagtcgc ctgcacgttc acgggtgcga tcgcgttcgg gaggatgtgc
     2821 acgccgagga gccgcggccc cgtcgcgccc aacgaccgag cggccagcac gtagtcggcc
     2881 tcgcgctggg cgaggatcga ccctcgtaga aggcgcgcga agacgggaac gttgatgatc
     2941 ccgatggcga gcatgatcgg cagcagcccg cgcccggtcg ccgtgacgat cgcgatcgcc
     3001 agcaggagcc cggggatcga cagcatgatg tccatgaacc tcatgatcac ggtgtcgacc
     3061 cagccgccga ggtagccggc cagcgcgccg agcgcgacgc cgaacagcag gccgatggcg
     3121 accgacacga cgcccgtgag gagcgacagg cgggacccgt agacgacgcg ggaaaagaag
     3181 tcccggccct gctcgtcgag cccgaaccag tgctctcccg acggcccctc ggggttcgcg
     3241 gcgagcgagc ccacctgctc gttcgggtcg taaggggcga ccagcggcgc gaacactgcg
     3301 acgatcacga acaacacgac gacgatcgag ccggcgaccg cgcccgggtt gcgcttgagc
     3361 cggtcgaacg cgtccgacca cagccccgcc tgcccctcta cggcacgggt cttggcttcg
     3421 gcttcggcga cgctcaccgg taccggatcc tcgggttcaa gaacgcgtac gacacgtcga
     3481 cgagcaggtt cacgagcacg attatcaggg cgaagaagac gagcccgccc tgcagcaccg
     3541 cgtagtcccg gaagctgatc gcgttgatga tcatcgtgcc gagcccgggc caggcgaaga
     3601 cccgctcggt gaggatcgcg cccgtcagga gcagccccgt ctggaggccc acgatcgtga
     3661 ccacgggcag cagcgcgttg cgcatcacgt ggcggcggtc gaccaccgag ggcgccaggc
     3721 ctttggcgcg cgccgtccgg acgtagtcct cgctcagcac gtccagagtc gcggccctcg
     3781 tgatccgcgc gacgatcgcg agagggatcg tccccagcgc gatcgccggg aggacgaggt
     3841 gccggaccgc gtccatgaac gccgggaagt ctccggcgat caaggcgtcg agcgtgtaga
     3901 agccggtcgg gtgctcgagc gtccgcgtga cgtcgagccg cccgatgctc gggagccagc
     3961 cgagcttcac cgcgaacacg tatttgagga tcagcgcgag gaagaagatc gggaagctga
     4021 tcccgatgag cgacagcacg aggctcgact ggtcgaggaa tccctggtag cgcttggccg
     4081 ctacgtatcc gagcgggacg ccggcgagga tcgcgaacag catcgcggcg aacccgagct
     4141 cgatcgtcgc ggggaacctg tccttgagct cgtcgaccac gggccggcgc gtctggatgc
     4201 tcgcgcctag gtcaccggag aggaggttgc cggcgtacgc cagatactgc tcgtggacgg
     4261 gcttgtcgag gccgagctcg cgctcgatcg cctcgaccga ctcctgggtg gcgcgctcgc
     4321 cgagcagcgc gatcgccggc ccgcccggca gcgaacgcac gaagacgaag acgaggatag
     4381 agagcccgat caggatcggg accaagagca cgagccgtcg gacgacgtat ctcagcatct
     4441 agccggtcac cttatccaga acgtaaagag ggggcgcgtt acgcgccccc tcttcgagtt
     4501 cgatcgctgc ggcttaggcg ccgccctcga ccgtcacgat gctgaacggc tccagcgaca
     4561 ccgggctcgg ctggtagccc gtgacgttcg cattgaacgc cagcgccggc ttcgtgtgga
     4621 cgtacgggag acccggcagg aagtccatga tcagccggtt gcactcctcg tacttggcga
     4681 cacgagcgtc ttggtcgacc tcggtctccg ctgcgtcgag gcagtcgaag atctccttgt
     4741 tgtcgaagcc ccaggccggc tgctccgtct ggaagaacgt cccgacgaag ttgtccgggt
     4801 cgccgaagtc accggtccac ccgagcaggt agagcccgta gcggccgttg tcgacgttgt
     4861 cgaggtagtc cgggctccag atcgccgact tcttctccac cttgaagcag cactcctcga
     4921 ggtccgcgac catcgcctcg aagttgtcct gcgggacggg catgtacggc cgggagacgt
     4981 cggtcgggta ggcgaagtcg atctcgaccg gcagcgtgta gcccgcgtcc tcgaggatct
     5041 gcttggcctt gtcggggtcg tagtcgtagg tcgtgacgtc gtcggcgtag ccgaagacct
     5101 ccggcggcat gaactgggtc gccacctcgc cctggccggc gtagaacgcg tccacgacct
     5161 cctggcggtt gatcgcgtgt gcgatcgcct tgcggacctc gatgtcgtcg agcggcttct
     5221 gcgcctggtt catcccgatg tagccgacgt tgaacgcggg gcgctcgagc agctggaggt
     5281 ccgggttgcc cgagatcgtc tccatgtcct ggggctcgac gaggtcgtag ccctggatct
     5341 cgcccgtctc gagggcctgg aggcgggcgg cgttgtccgg gatcggctgg aagatcagcg
     5401 tctggatgtt cccggtggcg tcttcctcgc cccagtagtc ggggttgcgc gtcatcgtca
     5461 ggtggtcgtt gcggacccac tcctcgaaca tgaacgggcc ggtgccgatc gggtgctcgg
     5521 tcccgtaggt gccggtcgcc cggaagatgc cctcggcgtt gacgcggccc tcgttggcgt
     5581 cgtactcctc gagcgccttc gggctgccga acgtgaagtt cgtgagcgcc atcgcggcga
     5641 ggaaggaaga cgacgggttg ttgagcgaga tcgtgaccgt gtgctcgtcg tcgaccgtgc
     5701 agctcttgaa gttggactcg gtcggcgccc cggagtcggg gtcggtcttc ttgaagccgc
     5761 cgaacaccgt ctgccagtag taggtggcgg ccgggttctg gaacgagccc tcgaagttgt
     5821 accagcggtc gaagttgtag cagacggcgt cggcgttgaa cgcctcgccg tcgtggaacg
     5881 tgacgtcgtc ccgcaggttg aaggtccagt cgagaccgtc ctcggacgtc gtccagtcgg
     5941 tggccaggtt cggcacgacc tcggtgccgc ccggcgcgag ggtcacgagg ccctcgaaga
     6001 tctggtcgat ggcccgcagc gactcgccgt cggagacgag ggcgccgtcg aggacgaccg
     6061 ggtcggccga cgtggcgaac gtcagggtcg cggtcgcagc ccccgcgtcg tcaccgccgc
     6121 cctcctcttc ggactcgccg tcgtcgcctc cgcaggccac tagcgtcagg gccaacacga
     6181 gcgcgagcgc gagcagccgg aatggtttcg gcatggactg aacctccccc ttcagcaggg
     6241 ctctcggaac ggagcctctc gaacccgcgc agtatatgtc cgctatcctg gccgggctac
     6301 tacctttgta gttgcggccc cccgcccgat tggttatcgc ggcgcgctgc tacccttggg
     6361 cgcgcccggg acgagagcgt tcgaacgaat cggcaggggg ccgagtccgg gtagacacga
     6421 cttcctcgga aaggggcaca ccggatggcg caatccccgc ctccgtcgaa cggcgggcgc
     6481 gacttctccg agttccaggt ggcattcgac cggatcgtcg acaacatgga gcggacgatc
     6541 cagggcaaga ccgggaccgt ccggctcgct ctcgtcacga tgatcgccga gggccacgtg
     6601 ctgctcgagg acatccccgg cgtcggcaag acgatgctgg cgaagtcgct cgccaagtcg
     6661 gtcgggtgcc agtggcagcg gatccagttc acgcccgatc tgctcccgag cgacgtcacc
     6721 ggcgtcaacg tgtgggaccg ggcctcgggc gagttcgtct tcaagcccgg cgcggtcttc
     6781 gccaacatct gcctcggcga cgagatcaac cgggcctcgc cgaagaccca gtcggcgctg
     6841 ctcgagtgca tggaggagcg tcaggtgacc gtcgacggga cgacccgggt cctcgacatg
     6901 ccgttcatgg tcatcgcgac ccagaacccg atcgagcacg agggcacgta cccgctgccc
     6961 gagtcgcagc tcgaccgctt catgatgagg ctctcgatgg gctacccgaa ccgcgagtcg
     7021 gagctcgaga tcctcgagac ccacggccgc acctcgctcc tcgaccagct cgagccggtc
     7081 gcggacgcga acgaggtcaa caacctgatc aagctcgccc gcgaggtcca cgtggcgccg
     7141 tcgctgcgcc ggtacatcgt cgacctggcc gaggcgaccc gcgaccaccc cgacgtgtac
     7201 ctcggggcca gcccgcgcgc ctcgctgttc ctcctgaagg cgtcgcgtgc gctcgccgcc
     7261 agccggggcc gcgactacgt cgtgcccgac gacatcaagg acctcgtcgt cccggtcctc
     7321 gcccaccgcg tgctgctgtc gcccgaggcg cagatgcgcg ggagcagtgc cgaggccgtg
     7381 ctcgagggcc tctcgggccg ggtcccgatc ccggccaaag agcaggccta accgcgatga
     7441 ggcgccccgc cccatgctga cgcttcgagg ctgggccgtc gccctcaccg ggctcgcact
     7501 cctcgtcgtc gcgcgcatct tcgggaccgg ggccgtcgag cagttcggct tcgcgctcgt
     7561 cgtgctcccg ctgatcgcgc tcgccgtcgt gcgcctgggc aagcacgacc tcgaggtcac
     7621 gaggcggacc tcccccgagc gggtcagggc ggggagcaag gtccgggtct cgctcgagat
     7681 ccgcaaccgg ggccgcggcc cggcccccct gctgctcctg gaagaccgcg tcccgagcga
     7741 cgtcgccggc cgggcccgct tcgcgctgca cggcgtcgag cccaaggggc gccgggtcgc
     7801 ctcctacgag ctccggcccc cgcggcgcgg caactaccag gtgggcccgc tgacgctcac
     7861 ctcgaccgac cccttcgggc tggcccgtct cgagtcggcc gcggccccca aggcctcgat
     7921 cctcgtccac ccgcgcgtcg agccgctgac gctcccccgc gacctcggcg accggcgcag
     7981 cctcgcggtc tcggccctcc gccagcccat cggcgcgcag ggcgaggact tctacacgct
     8041 gcgtgagtac aacgagggcg acgacctccg gaaggtgcac tggccggcga ccgccaagcg
     8101 cgggcgctac atgatccgcc aggaggagac gccctggcac aaccgagcga cggtggtctt
     8161 cgacgaccgg acgaccgcgc acgacggcat cggcgacgcg agcagcttcg agcgcgtgat
     8221 cgaggcgacc gcgtcgatcg tcgacctcta ccagcggtcc gggtacacgt accggctcgc
     8281 ggggggcgtc tcgaacggcc tgccggcgtc gaagggcacg ccgcacttca accgggccct
     8341 cgacctgctc gccacggtcc gcacctcgcc gccgagcgcc ctcgaagccg atcccctgat
     8401 cgcacggctc ctcgagatcg acgcgctgac gtccggcgag ggcacgctgg ccgtcgtcac
     8461 cgggtcgctg acgcccgacg ccgcgatggc gatcacgcgc tgccgccgcc ggttccgcca
     8521 ggtgatcgtc gtcgtcttcc ccgcgcaccg cttcgggacg ctgacgacga aggcccggtg
     8581 ggcgggcgag gaggaagtgg gcgaggtcgt gaagctgctc gggcgggccg gggcccaggc
     8641 gatcgtgctc ggccccgagg agtcgctgct gtccggctgg gactcgctga cccgctcggc
     8701 ctcgtcggcc gtcaggtcca agcccggggc gaccgtcagg tgagctcgga gggccgcgcc
     8761 cgggtcgggc tctcgatcct gctgctgctg acgctgttct cgttcgcgca ggtcttcgcc
     8821 gagggcgact atccggggcc cgtcctgctc gggatggtgc tcgcgtcggc gctcgcgatc
     8881 ggggcccggc ggctgggcct gtggacgagc acgacgatcg cggggagcct cgccgtcctc
     8941 ttcctctacc tcgtcgcgat cttccaggcc cgcaactcct tctacctgct gccgtcgccg
     9001 gacgcagtgg ccgggctcgg ccgctcgatc gggaacgcgc tgcgccactc gtcggtcgac
     9061 tacgcaccgg tcccggtccg cacgggctac gtggtcctcg tcgtgatcgg gatgtgggtc
     9121 gcgacgacga tcggcgagat cgccaccttc cgctggcggc gcccgctgct ggcggcgctc
     9181 ccgtgcatag ggctgttctg catccagatg acggtcggga ccgggacggg cgctccggcc
     9241 ctcgtcatcg cgttcctcct cgcgctcttg acctactggg ggctcgaggc cgcccaccgc
     9301 gtgcgctcgt ggggccgctg ggtcgccgcg ttccccggga ccgggcggga cggcaaggag
     9361 ccggagtccg tgaccggcgc tctggcgcgg cggatggggg ccgggtgcgt cgcggccgcg
     9421 atcgtggcgc cgttcgtcgt ccccgcgctc ggcgacgggc tgctcgcctg gcgcaacgag
     9481 accggcgagg gcgggctcgg ctccggcggc gggtcgggcg agatcaaccc gttcgtgtcg
     9541 atcgccccgc ggctcatcga gcagaccgac gaggtcctgt tcaccgtgcg ctcggagcgg
     9601 cccgagtact ggcggctgct ctcgctcgcg gacttcgacg ggacgcagtg ggacccggtc
     9661 ccgtcttccg acagcgaggt cgtcgccgac ggcgaggagt tccgccgcca gacgaactcg
     9721 ccccagagct ccgagccgct cgagcacacg atcacgttcg cggccctcga ctccacgcgg
     9781 ctccccacgg cgctcaaccc ctacgtcgtc gacttagagg acggcggcga gttcacctac
     9841 gacggcgaga accaggagct gacgctcgac ggcgaggcgg gcgagggctt cgagtacgcc
     9901 gtcgtctcga acgtcccgga cctctcctac gaccggctgc gcaaggccga ggtcggcttc
     9961 cccggccagg tctacgcgga cgacgggcac gtctcgcccg acgtggacgc cctcgccgcg
    10021 gacatccgca ccacggcgaa cggccgggcc ctgtcggacg ccgactacct catcgccctc
    10081 caggagtacc tccggggctt cacctacgac atcacgcccg aggatgcgga gctggccgcc
    10141 agcggagggt ccgtcgactt cctcgagagg ttcctcttcg acacgcagac cggctactgc
    10201 cagcagttcg cgaccgcgtt cgccctgctc gcccgggccg agggcatccc gacgcgcgtc
    10261 gccgtcggct tcctgccggg ctcgtccagc caggaggagg acgacaccta cgtcgtgtcg
    10321 ggcctccaca cccacgcctg gccggaggtc tacttcgacg gctacgggtg ggtccgcttc
    10381 gacccgacgc cccgtctcga ggcgccccag ccccgttaca cgcgtgagcc gatcgaggcc
    10441 ggggggccgg gcacggccgg cggtaccgag aacggcggga tcggcgccgc cctcggacgc
    10501 caggacccga tcgaggaccg gcggctcgcc ggcgccaaca cgggtgcggc gacgggggcc
    10561 gacaggcccg ccggcaccgc catcggcgac cgccgcgtgg ccgaggaccc cgtgtgggag
    10621 aaggcgttca accgggtcgc gctcgggggc gcgctcgtgc tcgtgctctt cctggcgtcg
    10681 gtccccgcgc tgaagacgtg gaggatcagc cgccgctacc gcagggcccg gggcgacgac
    10741 ggcgcggccg cagcggcttt cgcccacttc gaggacgagg ccgcggagct ggcgtcgccg
    10801 cgcgacccgg ccgagtcggc ggtggcgtac gcattgcggc tggccgggct ccgcaaggtc
    10861 ccgaaggaag acgcgacgcg cctggcctcg atcttcgaag cggccgagta cgcccccggc
    10921 gggatccgcc cggaccaggc cgggcaggcg agggcgctgg cgcgcaagct caggggcgcg
    10981 ctgtgggcac gtgcgtcgtg gtgggcgagg gccgggaggc tgttctcgcc gcggcggcta
    11041 gttggccgcc cgtagcctcc gcagcgcctc ctcgggcacg tcctgctcga gcttctcctc
    11101 gacctgtcgc agagcggtga cgaactcctc gcggtcgtag aggtactcgg ccacgcgtcc
    11161 gtcgacgccg gcggcgatct cgtcgatggc gatgaagaag gcgagctggc cctccttcaa
    11221 cgcgtcgagg agctcgccgt cgttgcgagc gatcttgaag atgctctgcc cgtcggtgac
    11281 gagcttgacc tccgagaggt ggtcgtcgag cgcggcccgc ttgcgcaggt acgagtacgc
    11341 gcggcgaacc ctctggaggc tcatgccgtg gtcgaggagc gacttgacca ccttcaaagc
    11401 gacgaggtca cggaacgagt agagccgccg cacccccgga cgtcctccgg tggcctggac
    11461 gctcggcttg acgaggccga tgcggtccca atacctcagc tggtgagccg tgcaacctgt
    11521 gaaacgagac gtttgctgtg cagtgaaacc ctccatggat gagtacgtcc ttccactaca
    11581 agagcaagga aaaagccttc gacacgcctt aggagatccc aacccgcgac gggcaatgtt
    11641 gcaccgcgaa gtgagcc
//
LOCUS       DASQDO010000024         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_431985, whole genome shotgun sequence.
ACCESSION   DASQDO010000024 DASQDO010000000
VERSION     DASQDO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431985"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VEV43_00735"
     CDS             complement(<1..974)
                     /locus_tag="VEV43_00735"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HYP22079.1"
                     /translation="MLTAGAVSYATESLVVEVDGAPLAPDLAARLLAVTVDDCTAQPG
                     AFSLSFRDPFHDAVEKGRFAFGASVTVALADGTVLLSKAELTARDSEVDPAGTITVVR
                     GFDLSHRLHRGRATEIYRQVRYSDVARTVARRASLETGRVDTPRTAVKPQVTQCNASD
                     WDFLTSLAREIGYEVAVVDGRLTFAPRPEASSASVTITPDDLVRLRCVVSAAEQAAQV
                     EVRGWNPQLKQEVVATAAVAPRGSELAEAPAAAASKVGGATLTSVAGLYHDTRDATAA
                     AGALAEQVGDAYGTLEGVAHGNPGLRAGTVVSLEGAGAGFDGDYTLTAT"
     gene            complement(977..1636)
                     /locus_tag="VEV43_00740"
     CDS             complement(977..1636)
                     /locus_tag="VEV43_00740"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYP22080.1"
                     /translation="MAKAYLEFEEWAAAAAGGAPRRIEFQFNPKEFSVKQGAHWVEVD
                     GSTASEYVGPRSQHMSVGMFLDASDAPDGDVSAQVDTLLAACHPTPASSSKSQPLPPR
                     VRFGWDRVHFEGYVSDVDVHYRLFHPDGRPIRAECTIEMQEIERPTPRQNPTSGTPEI
                     HRTVAVVAGDTLAGLAYREYGDPARWRAIAELNGVDDPLRVRPGTRLLLPPAHRLREA
                     S"
     gene            complement(1639..2088)
                     /locus_tag="VEV43_00745"
     CDS             complement(1639..2088)
                     /locus_tag="VEV43_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYP22081.1"
                     /translation="MTASDPTTPGTVCALFSVVIDDIDGSYDLGTFVSCEGIGLTVEV
                     EDRVEGGNNGFVWKLPTRVSYGNVTLTRPPGPDTAKLLEWFASLRNGVRRTTGRIVAL
                     AFAESDRVEWTLEGVVPVRYQGPSFSADSTSPALETLEIAHHGFKVK"
     gene            complement(2085..>2408)
                     /locus_tag="VEV43_00750"
     CDS             complement(2085..>2408)
                     /locus_tag="VEV43_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22082.1"
                     /translation="RGGRIPPEDSAEGRELERQAGEIATSSRAPRRGQPALPPGVASP
                     PPPPAASEPMQRKPVDTASGDEPETQLDLDDLARRLYPRLRPYLRKELSLDRERAAAN
                     LRGLR"
BASE COUNT          310 a          869 c          891 g          338 t
ORIGIN      
        1 gtcgcggtaa gcgtgtagtc gccgtcgaag ccggccccgg ccccctcgag gctcacgacc
       61 gtgcccgcac gcagcccggg gttgccgtgc gcgacgcctt ccagcgtccc gtacgcgtcg
      121 ccgacctgct ccgccagcgc ccccgccgcc gcggtggcgt cgcgcgtgtc gtggtacagc
      181 ccggccacgc tcgtgagcgt ggcgccgccg accttcgacg cggccgccgc cggggcctcg
      241 gccagctcgc tgccgcgtgg ggcgaccgcg gccgtggcga cgacctcctg cttgagctgc
      301 gggttccagc cacgcacctc cacctgcgcg gcctgctcgg cggccgagac cacgcagcgc
      361 aggcgcacga ggtcgtccgg cgtgatcgtc acgctcgcgc tcgacgcctc gggacgcggg
      421 gcgaacgtca gcctcccgtc gacgaccgcg acctcgtacc cgatctcgcg cgcgagcgac
      481 gtcaggaagt cccagtcgct cgcgttgcac tgggtgacct ggggcttgac ggcggtgcgc
      541 ggagtgtcga cgcgaccggt ctcgagggac gcgcggcggg cgacggtccg ggcgacgtcg
      601 gagtagcgca cctgccggta gatctccgtg gcacggccgc ggtggaggcg gtgggacagg
      661 tcgaacccgc ggacgaccgt gatcgtgccc gcggggtcga cctccgagtc acgcgcggtc
      721 agctccgcct tcgacaacag caccgttccg tcggcgaggg cgaccgtgac cgacgcgccg
      781 aacgcgaacc gccccttctc gaccgcgtcg tggaacgggt cgcggaacga cagcgagaac
      841 gcaccgggct gcgcggtgca gtcgtcgacc gtgacggcga ggagccgggc cgcgaggtcg
      901 ggggccagcg gggcgccgtc cacctcgacg acgaggctct cggtcgcgta cgagaccgca
      961 ccggcggtga gcaccgttac gacgcttccc ggagacggtg cgcgggcggg agcagcaggc
     1021 gcgtccccgg cctcaccctg agcgggtcgt ccacgccgtt gagctcggcg atcgcgcgcc
     1081 agcgggccgg gtcgccgtac tcgcggtagg cgaggcctgc gagcgtgtcg ccggcgacga
     1141 ccgcgaccgt ccggtggatc tcgggcgtgc ccgaggtcgg gttctggcgc ggtgtggggc
     1201 gctcgatctc ctgcatctcg atcgtgcact cggcgcggat cggacgcccg tccgggtgga
     1261 acaagcggta gtggacgtcg acgtcgctca cgtagccctc gaagtggacg cggtcccagc
     1321 cgaagcggac gcgcggcggc agcggctggc tcttggacga cgacgccggc gtggggtgac
     1381 acgccgcgag cagagtgtcg acctgcgccg agacgtcacc gtcgggggcg tcgctcgcgt
     1441 cgaggaacat cccgacggac atgtgttgcg accgggggcc cacgtactcg gacgcggtcg
     1501 agccgtccac ctcgacccag tgggcgccct gctttacgga gaactccttg gggttgaact
     1561 ggaactcgat ccgccgcggc gcgccgcctg ccgcagccgc ggcccactcc tcgaactcga
     1621 ggtacgcctt cgccatcgct acttcacctt gaacccgtgg tgggcgatct cgagcgtctc
     1681 gagcgcgggc gaggtcgagt ccgcggagaa cgagggcccc tggtagcgga cggggacgac
     1741 cccctcgagc gtccactcga ctcggtcgct ctccgcgaac gccagcgcca cgatgcgccc
     1801 ggtggtccgt ctcacgccgt tgcggagtga cgcgaaccac tcgagcagct tcgcggtgtc
     1861 cggccccggc ggacgcgtca gggtcacgtt gccgtagctg acgcgcgtcg gcagcttcca
     1921 cacgaacccg ttgttgccgc cttcgacgcg gtcctcgacc tccacggtca ggccgatgcc
     1981 ttcgcagctc acgaacgtgc cgaggtcgta gctgccgtcg atgtcgtcga tgacgacgga
     2041 gaacaacgcg cagaccgtgc ccggcgtcgt ggggtcgctc gccgtcatcg cagcccccgc
     2101 aggttcgccg ctgcacgctc acgatcgagc gacagctcct ttctcagata gggacggagg
     2161 cgggggtaca gacggcgggc caggtcgtcg aggtccagct gcgtctcggg ctcgtcgccg
     2221 gacgccgtgt cgacgggctt ccgctgcatc ggctcggatg cggccggcgg gggcggcggg
     2281 gacgcgacgc cgggcgggag cgcgggctgc ccgcgcctcg gtgcgcgcga cgacgtggcg
     2341 atctcgccgg cctgtcgctc gagctcgcgg ccttcggccg agtcctccgg tgggatccgt
     2401 ccgccccg
//
LOCUS       DASQDO010000025        18321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_65070, whole genome shotgun sequence.
ACCESSION   DASQDO010000025 DASQDO010000000
VERSION     DASQDO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65070"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VEV43_00755"
     CDS             <1..378
                     /locus_tag="VEV43_00755"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HYP22083.1"
                     /translation="AQEGLAFVGISMADVLGDSIEVTFKTGEIAGPSAGLMFSLALYD
                     RLTPDDLTEGRKIAGTGTIGCGGVVGPIGGIEEKVAGAEDQGAEIFLAPELNYEAARD
                     AAGDIEVVSVATFDDALEYLEGL"
     gene            435..3236
                     /locus_tag="VEV43_00760"
     CDS             435..3236
                     /locus_tag="VEV43_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP22084.1"
                     /translation="MFESFMAVPLHFTVEFLGFLMTAGAALLVLSRPSLVAGEGFNRA
                     TAALGFVVLAAAQVAHGGAFAFGDLDATTDGAEFLASARGLGYALVKIAVVGGLRAAP
                     AAAAAVSLREPLLLVPAGAAALLSIMALAAARVDHAMRRLAAAAILIAAAEGLTASVP
                     GEVYGTGDVDSLSYLAHGLRGAGYLLLGAWLWTGIRSSIRTRFVASFVALLVAVVLAL
                     STALTGVISSNVERNELDRIEEQASSTAESIMVDETRDLLRLVDSFSRLNKDIAEEMA
                     TTRNLDPTAAQLVDQFPIDFAIVSANTRALGSAGEGPSFHQGAGPRFTERPLAADATV
                     TRLLGSDLIKDTEITDQVVGLDRVEDSIVVLGVRSVVDPAGRRVGFVMAGQWVDGLTM
                     ERITSRVKPTRASLVVNGRIVTTELPPKVPTRRLLPRPVRLETEATGEPTSMQQTLGT
                     RSFYNGIAALERPNGEVIGEIVLSSPAEIVSQTRQGVTRILFVVAMAVGAVAMVLAWL
                     SGRRITRPIQQLTATAGRVREGDLSAQAAVGGEDEVGQLGETFNEMTASLVRMTDDLR
                     EAALEESRLRTRIETIMQSMADGLVAVDADLRVLAFNREAESLTGLKAEQAIGKPVEK
                     VLEARDPNGDPVRLPIFDLAEGSVGGITLKRKGADPVPVAVTSAALRGDDGTVAGAVA
                     VVRDMTREREVERMKSEFLSNISHELRTPLTPIKGYAEILSRKEVPPEKAKQFVKGIL
                     DSTSRLERIVELLVDFSAMEAGRLSPKSTPIDLGALVAKLAADWEKKTPRHEVVAEVP
                     PKLPQVVGDERLIRRSIEEVLDNAVKFSPHGGTIRLEAKTATSGTNGRRRQVQVCVSD
                     EGIGITPEDLPKVFSDFHQLDGSETRAYGGLGLGLAFVQRIVEAHAGSITVESEPDQG
                     TTFTISIPAARRVKKPADN"
     gene            3288..4583
                     /locus_tag="VEV43_00765"
     CDS             3288..4583
                     /locus_tag="VEV43_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22085.1"
                     /translation="MGSKSTFVERIAHSRAPQIAVGLVLVLFVGVAWWRFSDASVLEE
                     MTVKKRAGIVTVLRGGETIEVGDSTSLKPRDVVQTHSSGEAVIRLEGDRLLTLAPDSK
                     VRVRDARGVESQGGSLLADTSESLTVTFGGTRASTSSATIRIDRGISAARVGSYEGRV
                     TLSAPGEERLVIGRLFQASPSVNFMPESAVPYDFNVADQWDRLHLDRVIALQEELDQL
                     SAGLETQVEGERPGLDYFSALQDGADVSFLKPYLRRDPINLLVGFTIASHEDGPLGAS
                     FRRAFDLFDRGAEWSVAATIMGVKLEAVLADLEDIATVAVEAASGGDDSFTAAAAVLA
                     EGGKLPPPGTGPVPTNAPPGTNPPPTQPPPSPTDKPNPPDECSNVVTCGAEDLTEPLE
                     PTPSPSPGPTDDPDEGEDEGPLQILNQGGKGIKDAVGGL"
     gene            4599..4970
                     /locus_tag="VEV43_00770"
     CDS             4599..4970
                     /locus_tag="VEV43_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP22086.1"
                     /translation="MSRPLVLVAEDDFNVRLTLEIVLQDEGFDTVMAADGEEALKAAR
                     SSSPDVILLDQIMPKLDGKQVLHALRQDAATRDIPVFVLSGMERGVPDDWPGAQFVGK
                     PFSPEELVRRIRAALPADKFV"
     gene            5019..7820
                     /locus_tag="VEV43_00775"
     CDS             5019..7820
                     /locus_tag="VEV43_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HYP22087.1"
                     /translation="MAPPARRSRRRAILIGAVVLLLLAASSLARFYTDVLWFQEVGFE
                     SVLWTSIGTQVWVGLAVAVAIAAIVWVNLVVAARAAPVYGGFVVGGRTIVDPMERYRD
                     VLTPYVRSLRFAVAAAIGILAGIGASAAWRTVLLWLNRVPFGERDPQFGRDVAFYVFD
                     LPFYRTALDLLWFALIASLLVTLVAHAFHGSLRPQIGLRGVTPGALAHVSVLLGLMAL
                     VKAVQYYLGTFGLNFSERGVVTGASYTDVNAHLPALRLLAIISIVSAVLFIVNIRVRR
                     LSLPLAAVGIWVLTSFLAGFVWPTAIQRFSVDPQELQQEREFIARNLEATREGFDLSD
                     VEQRPYAVATEITPEGLEASSNILANVRLWDPDTLGQSYEQRQAIRLYYTFPGVDVDR
                     YEIDGETRQVLLAARELSLDQLPAGSRGWQNQHLQYTHGYGVVASLANSIGPAGQPDF
                     LVSDIPGTVEEGAEDLALDQPRVYYGEEFEPAEYSIVNSGQAEVDYAAEEVVRNNYEG
                     EGGIELSSIFRKLAFAIRESDPNIVLSDLIEPDSRILFYRNVRDRVMRAAPFLSFDSD
                     PYAAAVDGRLVWILDGYTSTPWYPYSQRFDANAVVAGSEEPGVLRGSVNYVRNSVKVV
                     VDAADGTMEFYVVDEDDALIRAWRAAFPDLFTEEEPSADLQAHFRYPEDLFNLQTEVY
                     RTYHMQEPDDFFAKEDAWDIPENPATYLLTQLPGEIDEEFVLTRAATPRGKRNMISLM
                     VARSDPEAYGELISLRFPAQNPPNGPGQVESLISQDEEVSQAETLLGQEGSDTFFGSL
                     IVLPIEDSILYVRPFFVTAEGVGIPELKRVAVVLDDEVAFEDTFDEALAELFGLGTPE
                     PPPEPGEPEEPEEPGDPEGPASPELMEIVEEAGRVYEAAQEALAAGDFEEYGRLIEEL
                     GELLERAGSLSGGAETGSG"
     gene            complement(7817..8281)
                     /locus_tag="VEV43_00780"
     CDS             complement(7817..8281)
                     /locus_tag="VEV43_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22088.1"
                     /translation="MDGPSPVELRRRRMFDPLLERRSLGGFRSVELNLAGWATLVAAG
                     LAAGALSVIAGSPAKIALPAGVVAAWLVALVLDTVHWRNMTTGIGRDGLDPVTGDEIV
                     RRLAERGIEAEYQEQVFDEPDGTLYLQRNIRCRNADSAKVRALVDEVLGRKT"
     gene            8312..8388
                     /locus_tag="VEV43_00785"
     tRNA            8312..8388
                     /locus_tag="VEV43_00785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8346..8348,aa:Met,seq:cat)
     gene            complement(8429..8656)
                     /locus_tag="VEV43_00790"
     CDS             complement(8429..8656)
                     /locus_tag="VEV43_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22089.1"
                     /translation="MDEMRLHVELFYDDEVRQWGYTVPALSILGTGCDSRADAERFAV
                     EAIRDVIESGEEELDADADILTLDVQIARAS"
     gene            complement(8661..8867)
                     /locus_tag="VEV43_00795"
     CDS             complement(8661..8867)
                     /locus_tag="VEV43_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYP22090.1"
                     /translation="MPKAARVLAALKRDGWVEVRRRGSHVTLEKNGRRVRWTHHHGTD
                     LGGPMMAKTARSFGYTLEELRRMV"
     gene            8997..9578
                     /locus_tag="VEV43_00800"
     CDS             8997..9578
                     /locus_tag="VEV43_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22091.1"
                     /translation="MLRPTVRVAVAAALVLAASLPALGAGASTDPERPACSHGYRRSR
                     EVAHGRFLGRRWELVFFRDRKDRPCLSDSWGPYGSIFRFRVREHRPRLGIVNLNAPTP
                     PDGPGAYVFEAYVQERVARATFTIDGRTRDVRIVRSPRWTRLPKNLLIHFVGTRRYNR
                     DATGRLRAFDSEGRLLATRVLRRGQFSKSAEPQ"
     gene            9638..10966
                     /locus_tag="VEV43_00805"
     CDS             9638..10966
                     /locus_tag="VEV43_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP22092.1"
                     /translation="MKTTRSIRRALGALVAAALVVPLSQAPASAKAEAPDEPRVVVAV
                     IDTGFNAFHEYFYKGGPLYGNKGPSSVTPAVLKEFGIGKDQIIRVPRTGDFGKDFAKV
                     QAKFDAIEKGEPYWFAGTNVIGISFLGDKQRLRPDGNASSHGIGTAASVLTANPEAIV
                     VAVEDWNVDAAEKWAFTHPAVDIVSTSYGPATSYPTFTNLSFSYTGVVENGKVHFGAA
                     ANDPTYAGIDETGGPWWSIGVAGFEEGASGGRQLLSGQVPDFVGDFTQDLPYCRSCEQ
                     GLQSVGGTSFATPRSAGTFSKVLLETRRAAGHDGGIVKPGKGAPLLVDGKMKLTNWDM
                     RRALEEAAYYPTTGEYGTEGGADPTSVPVNDAAPWVQTGWGLISPARQFEVVKEMLAH
                     LGVAGKPTRTKSQEACDFMTANMEARRAYWNDLAMNGESFGIGDDPYVYC"
     gene            complement(10982..12865)
                     /locus_tag="VEV43_00810"
     CDS             complement(10982..12865)
                     /locus_tag="VEV43_00810"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22093.1"
                     /translation="MPTPPRPTARQRLRYWFDNTLSSGTAAMVLWLTLATVGLILVIA
                     TAAAIAEVRAEGAELSFGEALWSTLMRTLDPGTMGGDVGWPLRVASLAATIGGILIVS
                     SLIGLLANGISDRVAELRRGRSPVLETGHTLILGWSPKVFPIISELTVANESQGGGAV
                     VVVADMDKDEMERLLDARVKERRGTRIVCRNGVPFEPLDVAIARPEAAKSIVVLNPGH
                     ADGDAEVVKTALALIRTAKLAGDVPVVAEVSDAFTAAALRDGTAGAVSVVRSTSIIAR
                     VTAQVCRQPGLSDVYQELFDFAGDEIYFAPAGKLAGSTFGAALTSFEQAVPLGLRFGD
                     GRVALNPSADTPISEGDRLVAIASDDDAIVFTGAGAGSASRGEAVAARTEAERILMIG
                     WNRMAPEIVRELDNYVAPGSVLSVHVDPVVVPAEEVDVPETRNLSATVHTEALDPDAL
                     RTIMGSQAPEHVIVLCYKERLSEAQADARVLLTLLHLRAAIDQAGAPTSVVAELLDER
                     DVDLSPSGTTDEFIVSERLTALIMAQLSENRELAPVFDDLLDEAGAEIYLKPASLYAP
                     PATQVSFGDLTASAAARGEVALGYQAWAGGDGRKVTINPPKGRTFEARADDRLIVLAE
                     TQG"
     gene            12907..13572
                     /locus_tag="VEV43_00815"
     CDS             12907..13572
                     /locus_tag="VEV43_00815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HYP22094.1"
                     /translation="MDGERFAEVDRYLAALLAPPDGELTGALEASAAAGLPPIEVTPT
                     EGKLLHLLARLSGARSILEVGTLAAYSTIWLARALPAGGRLVTIEADPVHAEVARRNV
                     GRAGLDGVVEVREGAALDVLPDLVRDGAGPFGFVFLDADKPNNPAYLEWSLRLTAPGS
                     VIVADNVVRGGAVASATDDPSAAGARRFLEMVAAEPRLEGTAVQTVGTKGHDGFALAL
                     RVS"
     gene            13733..16351
                     /locus_tag="VEV43_00820"
     CDS             13733..16351
                     /locus_tag="VEV43_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYP22095.1"
                     /translation="MTDVIASERRIGDRFRFLTGEQKVWMFNALLAGCAAAVYFSFVV
                     DIPTPSAPFHLSWWVLAPLFAFSEICVIHVQLRREVLSYSLNEIPLILGLFFASPPDL
                     VLGVLAGGGAALVLHRRQAMLRLTFNLAHFWLTSCIAILIFHSVVGNHAHTGPAGWLG
                     AFLGGFTADTIAAFCVVLVMSLAEGRRQSLKALIGPGWIITLTNACVALAAVTVLWVD
                     PVSAWLLAVPAALLFLAYRGYNAQRHKRQSLELLYESTRILQQSLEANSVVDTVLEQA
                     RQMLRADYAEITRFPTVKGGRAFRASLGPGVRREPLRSINLDPTEGVWARVAAEGQTI
                     LVARPIENEKLRTHFHERGIRDAIVAPLHGAEGIMGTLLVGNRLRDDLATFDSDDLKL
                     VETLANHASISLENGRLVESLKRQAAENEFQAQHDALTGLFNRTMFHNRVSQAIAQAG
                     SDMVGVLLMDLDRFKEINDTLGHPTGDSLLKETSFRLRRILGPDVTIARLGGDEFAVL
                     LPRLRSLIGATDMAGNLVSALREPYELQDLSLDVGASIGISLFPQHGTDAETLMQRAD
                     VAMYIAKASHSGFEIYDSDKDQYSPNRLALVAELRRAIEERQLVVWYQPKVDLGSGSL
                     IGAEALVRWKHPRHGMMPPDEFIGLAEHTGLIGPMTTYVLEEALQQCLAWRCSGFPDF
                     KVAVNLSVKNLMDVDLPSQVSALLNKSQLVPDALQLEITESSIMEDPERAIEVLTRLH
                     EMGIGIAIDDFGTGYSSLAYLRRLPVDEMKIDRSFVRGMMSSENDAVIVRSVIDLGRN
                     LGLVVVAEGIEDRESMERLEALGCTVGQGYHIARPMDVEGFDSWAASRHAAEAAAPPA
                     PPPPSVVPFPPRLQSL"
     gene            complement(16429..17217)
                     /locus_tag="VEV43_00825"
     CDS             complement(16429..17217)
                     /locus_tag="VEV43_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP22096.1"
                     /translation="MSWLRVKSIAKRHAYVLRRSPHRLYDITIWPLVDVLLFGSLGVF
                     LSEAEGGEAAAYGYLLAGIVLWHVVYQSQIALSTGFLEETWSRNLLNLMVTPLKEVEY
                     AAGVALFGLVKLALGVGLVALAALGFFAFDITDVGLGLIPIAAVLLAVGWAIALFVIA
                     LVLRFGSGAEALAWGILFLVMPLSGVFYPVPALPGAVQPIARLLPTTHAFTAMRSLLD
                     GQGMEWRELALAAGGAVALGLAATLFLAKMLSTFRTRGYITRYS"
     gene            complement(17214..17975)
                     /locus_tag="VEV43_00830"
     CDS             complement(17214..17975)
                     /locus_tag="VEV43_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP22097.1"
                     /translation="MEHVIRATGLTKTFHATKAVDGVDLTVSAGQRVALLGPNGAGKT
                     TTLFMLLGVISPDEGHVEVAGLRLPEHRSEAMERVGFAAGYMPLAERLRVREFLALYG
                     GLYGLKDPRERIELGLERFGITRLANVMGNELSSGQRTLVGIIKATLHGPRLLVLDEP
                     TASLDPDVAQRVRQGLHDLCEKEGTTLLVTSHNMVEVARLCDRVIFMSAGRIVADGSP
                     EEVAGRFGQDDLEDVFLHLASNARDASRAQETDHL"
     gene            18045..>18321
                     /locus_tag="VEV43_00835"
     CDS             18045..>18321
                     /locus_tag="VEV43_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HYP22098.1"
                     /translation="MRRDERREARFRALYGEHLDAVARYAARRVDHAEVHDVVSEAFL
                     VAWRRLEDVPADPLPWLFATARGVISNRRRSSLRRRALRDKLALQVAT"
BASE COUNT         2774 a         6497 c         6330 g         2720 t
ORIGIN      
        1 gcgcaggagg ggctcgcgtt cgtcggcatc tcgatggccg acgtcctggg ggacagcatc
       61 gaggtgacgt tcaagacggg cgagatcgcc gggccctcgg ccgggctgat gttctcgctc
      121 gccctgtacg accgcctcac ccccgacgac ctcaccgagg gacgcaagat cgccggcacc
      181 gggacgatcg ggtgcggcgg cgtcgtggga ccgatcgggg ggatcgagga gaaggtcgcg
      241 ggcgccgagg accagggggc cgagatcttc ctcgcgcccg agctcaacta cgaggcggcc
      301 cgcgacgccg caggcgacat agaggtcgtg tcggtggcga ccttcgacga cgcgctggag
      361 tacctggaag ggctttgacg aggtaagggc tcgggccggg ccccccggag gccgtcgtgg
      421 gagactgaac cacgatgttc gagagcttca tggcggtccc tctccatttc accgtcgagt
      481 tcctcggctt cctgatgacg gcgggcgccg cattgctggt gctttctcgc ccgtcgctcg
      541 tcgcaggcga ggggttcaac cgcgccacgg cggcgctcgg cttcgtggtg ctcgcggcgg
      601 cccaggtagc ccacggcggc gccttcgcct tcggggacct ggacgccacg acggacggcg
      661 ccgagttcct ggcgtccgcc cgtggcctcg gctacgcgct ggtgaagatc gcggtcgtcg
      721 ggggcctccg ggccgccccg gcagcggccg cggccgtctc gttgagggag ccgttgctgc
      781 tggtccccgc gggggcggcc gctcttctct cgatcatggc tctggcggcc gcccgggtgg
      841 accacgctat gaggcgcctg gccgccgcgg cgatcctgat agcggcggcg gagggcctga
      901 cggcgtcggt cccgggcgag gtctacggca cgggcgacgt cgacagcctg tcctatctcg
      961 cgcacggctt gcgcggggcg ggctatctgc tgttgggggc gtggctgtgg acggggatcc
     1021 ggtcgagcat ccggacccgc ttcgtggcgt cgttcgtagc tctgctggtc gcggtggtgc
     1081 tggcgctgtc gacggcgttg acgggcgtga tctcgtcgaa cgtcgagcgc aacgagctcg
     1141 accggatcga ggagcaggcg agcagcaccg ccgagagcat catggtcgac gagacgcgtg
     1201 atctcctgag gctcgtcgat tcgttctccc ggctgaacaa ggacatcgcc gaggagatgg
     1261 cgacgacgcg caacctcgac ccgaccgcgg cccagctcgt cgaccagttc ccgatcgact
     1321 tcgcgatcgt gagcgcgaac acgagagcgc tcgggagcgc gggcgagggc ccgtcgttcc
     1381 accagggggc cgggccgcgg ttcaccgaac ggcccctcgc cgccgacgca acggtcacga
     1441 ggctgctcgg cagcgacctc atcaaggaca ccgagatcac cgaccaggtg gtcgggctcg
     1501 accgggtgga ggactcgatc gtcgtcctcg gggtccggag cgtcgtcgac ccggccggcc
     1561 gccgcgtcgg cttcgtcatg gccggccaat gggtcgacgg gctgaccatg gaacgcataa
     1621 cgagccgggt caaaccgacc cgcgcctcgc tcgtcgtgaa cggccgcatc gtcacgaccg
     1681 agctcccgcc gaaggtcccc acccgccggc tcctgccgag gcccgtccgg ctcgagaccg
     1741 aggccaccgg ggagcccacc tcgatgcagc agacgctcgg cacccgctcc ttctacaacg
     1801 gcatagcggc cctcgagagg cccaacggcg aggtcatcgg cgagatcgtc ctctcctcgc
     1861 ccgccgagat cgtctcccag acccgccagg gcgtcacccg catcctcttc gtcgtcgcca
     1921 tggcggtcgg cgcggtcgcc atggtcctcg cctggctctc cggccgccgc atcacccgcc
     1981 cgatccagca gctcaccgcg acggccggcc gcgtccgaga gggcgacctc agcgcgcaag
     2041 cggccgtggg gggcgaggac gaggtcggcc agctcggcga gaccttcaac gagatgaccg
     2101 cgtcgctcgt acggatgacc gacgacctgc gcgaggccgc gctcgaggaa tcgcggctgc
     2161 gcacccgcat cgagacgatc atgcagtcga tggccgacgg gctcgtcgcc gtcgacgcgg
     2221 acctgcgcgt gctcgccttc aaccgcgagg ccgagagcct caccgggctg aaggccgagc
     2281 aggccatcgg caagcccgtc gagaaggtgc tcgaggcccg cgacccgaac ggcgacccgg
     2341 tccggctccc gatcttcgac ctcgccgagg gctccgtcgg cgggatcacg ctcaagcgca
     2401 agggggccga ccccgtcccc gtcgcggtca cgagcgcggc gctccgcggc gacgacggga
     2461 cggtggccgg cgccgtggcc gtcgtgcgcg acatgacgcg cgagcgtgag gtcgagcgca
     2521 tgaagtccga gttcctctcg aacatctcgc acgagctccg taccccgctc acgccgatca
     2581 aggggtacgc ggagatcctc agccgcaaag aggtcccacc cgagaaggcc aagcagttcg
     2641 tgaagggcat cctcgactcg acgtcgcggc tcgagcggat cgtcgagctg ctcgtcgact
     2701 tctccgcgat ggaggccggc cgcctcagcc cgaagtcgac tccgatcgac ctcggggcac
     2761 tcgtggcgaa gctcgccgcc gactgggaga agaagacccc gcgccacgag gtcgtagcgg
     2821 aggtcccgcc gaagctcccg caggtcgtgg gcgacgagag gctcatccgg cgctcgatcg
     2881 aggaggtcct cgacaacgcg gtgaagttct cgccccacgg ggggacgatc cggctcgagg
     2941 cgaagacggc gacgagcggg accaacgggc ggcgccgcca ggtccaggtc tgcgtcagcg
     3001 acgagggcat cgggatcacg ccggaggacc tgccgaaggt gttctcggac ttccaccagc
     3061 tggacgggtc cgagaccagg gcgtacgggg gcctcggcct cgggctcgcc ttcgtccagc
     3121 ggatcgtcga ggcccacgcc ggctcgatca cggtcgagag cgagcccgac caggggacga
     3181 cgttcacgat ctcgatcccg gcggcccgga gggtcaagaa gcccgcagat aactagtcac
     3241 aacggactat tccaataacc aggcgtcctg cgagaaacta cgcagatatg ggatcaaagt
     3301 cgacgttcgt cgagcggatc gcccactccc gcgcccccca gatcgcggtc ggactagtgc
     3361 tcgtcctgtt cgtcggcgtc gcctggtggc gcttctccga cgcctcggtg ctcgaggaga
     3421 tgaccgtcaa gaagcgggcc gggatcgtca ccgtcctgcg cggcggcgag acgatcgagg
     3481 tcggcgactc cacgtcgctc aagccccgcg acgtcgtcca gacgcacagc tcgggcgagg
     3541 ccgtgatccg cctcgagggc gaccgtctcc tcacgctcgc ccccgactcg aaggtcaggg
     3601 tccgcgacgc ccgcggcgtc gagagtcagg gcggcagcct cctcgccgac acgagcgaat
     3661 ccctcaccgt gaccttcggc gggacccgcg cctcgacctc gtccgcgacc atccggatcg
     3721 accgggggat ctcggcggct cgtgtcggct cctacgaggg ccgggtgacc ctcagcgccc
     3781 ccggcgagga gcgactcgtc atcgggcgcc tcttccaagc gtctccgtcg gtgaacttca
     3841 tgccggagtc cgccgtcccc tacgacttca acgtcgccga ccagtgggac cgtctccacc
     3901 tcgatcgcgt gatcgcgctg caggaggaac tcgaccagct ctcggcaggg ctcgagacgc
     3961 aggtcgaggg cgagcgcccc gggctcgact acttctccgc acttcaggac ggcgccgacg
     4021 tctccttctt gaagccgtac ctccggcgtg acccgatcaa cctgctcgtc ggcttcacga
     4081 tcgcctcgca cgaggacggg cccctgggcg cgtctttccg ccgggccttc gacctgttcg
     4141 accggggggc cgaatggtcg gtcgccgcga cgatcatggg cgtcaagctc gaagcggtcc
     4201 tcgccgacct cgaggacatc gccacggtcg cggtcgaggc cgcatcgggt ggggacgact
     4261 cgttcaccgc tgcggccgcg gtgctggccg aaggcggcaa gctcccgccg ccggggaccg
     4321 gccccgtccc gacgaacgct ccgccgggca cgaaccctcc gccgacccag cctccgcctt
     4381 cgcccaccga caagcccaac ccgccggacg aatgcagcaa cgtggtcacc tgcggcgccg
     4441 aggacctgac ggagcctctg gagccgaccc cgagcccgtc gccgggaccc accgacgacc
     4501 cggacgaggg tgaggacgag gggccgctcc agatcctcaa ccagggcggc aaggggatca
     4561 aggacgccgt cggaggtctc tagacctaga ctgggccggt gagccgcccg ctcgtcctcg
     4621 tcgcggagga cgacttcaac gtccgcctga cgctcgagat cgtcctccag gacgagggct
     4681 tcgacaccgt catggccgcc gacggcgagg aggccttgaa ggccgcccgc tcgtcgtccc
     4741 ccgacgtgat cctgctcgac cagatcatgc ccaagctcga cggcaagcag gtgctgcacg
     4801 cgctccggca ggacgccgcg acacgcgaca tccccgtgtt cgtgctcagc gggatggaac
     4861 ggggtgtccc ggacgattgg ccgggggccc agttcgtggg caagccgttc agcccggaag
     4921 agctggtccg ccggattcgc gcggcactcc ccgccgacaa gttcgtttga cgcggggcgg
     4981 cctcggccgg accgccggct agacgttact ctccggccat ggcccccccg gctcgacgct
     5041 cgcgccggcg cgcgatcctc atcggcgccg tcgtcctact cctcctcgcc gcctcgtcgc
     5101 tcgcgcgctt ctacaccgac gtgctgtggt tccaggaggt cgggttcgag tcggtcctgt
     5161 ggacgagcat cgggacgcag gtctgggtcg gcctcgccgt cgccgtagcc atagcggcga
     5221 tcgtgtgggt caacctcgtc gtcgccgcgc gcgcggcccc cgtctacgga ggcttcgtag
     5281 tcgggggccg gacgatcgtc gacccgatgg agcgctaccg cgacgtgctg acgccgtacg
     5341 tgcggtcgct gcggttcgcg gttgcggccg cgatagggat cctcgccggc atcggcgcga
     5401 gcgccgcgtg gaggaccgtc ctgctctggc tgaaccgcgt gccgttcggc gaacgcgacc
     5461 ctcagttcgg cagggacgtc gcgttctacg tgttcgacct cccgttctac cggaccgccc
     5521 tggacctgct gtggttcgcg ctgatcgcgt cgttgctcgt gacgctcgtg gcgcacgcgt
     5581 tccacggatc gttgcggccg cagatcgggt tgcgcggcgt gacgccgggc gcgctcgcgc
     5641 acgtctcggt gctgctgggc ctgatggcgc tcgtgaaggc cgtccagtac tacctgggaa
     5701 cgttcgggct gaacttctcc gagcgcggcg tcgtcaccgg cgcgagctac acggacgtga
     5761 acgcccacct ccccgcgctg cggctgctgg cgatcatctc gatcgtctcc gcggtgctgt
     5821 tcatcgtgaa catccgcgtg cgccgtttgt cgctgccgct cgcggcagtc ggcatctggg
     5881 tcctcacctc tttcctagcg ggcttcgtgt ggccgaccgc aatacagcgg ttctcggtcg
     5941 acccgcagga gctccagcag gagcgggagt tcatcgcgag gaacctcgag gccacccggg
     6001 agggcttcga cctgtcggac gtcgagcaga ggccgtatgc ggtggcgacc gagataaccc
     6061 cggaagggtt ggaggcgagc tcgaacatcc tcgcgaacgt gcgtctgtgg gaccccgaca
     6121 cgctcgggca gtcgtacgag cagcggcagg cgatccggct ctactacacg ttccccggcg
     6181 tcgacgtaga ccgctacgag atcgacggcg agacgcgtca ggtgctgctg gcggctcgcg
     6241 agctgtcgct ggaccagctg ccggcgggct cgcgcggatg gcagaaccag cacctccagt
     6301 acacgcacgg ctacggggtc gtcgcgagcc tcgcgaactc gatcggcccc gcggggcagc
     6361 cggacttcct cgtgagcgac atcccgggca cggtcgagga gggggccgag gacctcgcgc
     6421 tcgaccagcc gcgcgtgtac tacggcgagg agttcgagcc ggcggagtac tcgatcgtga
     6481 actccggcca ggccgaggtc gactatgccg cggaggaggt cgtccggaac aactacgagg
     6541 gcgagggcgg catcgagctc tcgagcatct tccggaagct cgcgttcgcg atacgcgaga
     6601 gcgacccgaa catcgtgctg tcggacctca tcgagcccga ctcgcgcatc ctcttctacc
     6661 ggaacgtgcg cgatcgcgtg atgcgcgcgg cccccttcct ctcgttcgac tccgatccgt
     6721 acgcggccgc ggtcgacgga cggctggtgt ggatcctcga cggctacacg tcgacgccgt
     6781 ggtacccgta ctcgcagagg ttcgacgcga acgccgtcgt ggcgggctcg gaggagcccg
     6841 gcgtgctgcg cggctcggtg aactacgtgc gcaacagcgt gaaggtcgtc gtcgacgcgg
     6901 ccgacggcac gatggagttc tacgtggtcg acgaggacga cgccttgatc cgggcgtggc
     6961 gcgcggcgtt cccggacctg ttcaccgaag aggagccgtc tgccgatctg caggcgcact
     7021 tccggtaccc ggaggacctc ttcaacctgc agacggaggt gtaccggacg taccacatgc
     7081 aggagcccga cgacttcttc gcgaaggaag acgcgtggga catcccggag aaccccgcga
     7141 cgtacctcct gacgcagctg ccgggcgaga tcgacgagga gttcgtcctc acgcgtgccg
     7201 cgacgccgcg aggcaagcgc aacatgatct cgttgatggt cgcgcggtcg gatcccgagg
     7261 cctacggcga gctcatctcg ctcaggttcc cggcgcagaa ccccccgaac gggcccggcc
     7321 aggtcgagag cctgatcagc caggacgaag aggtctcgca ggccgagacg ctcttgggcc
     7381 aggagggctc ggacacgttc ttcgggtcgc tgatcgtcct tccgatcgag gactcgatcc
     7441 tctacgtgcg gccgttcttc gtgaccgcgg agggcgtcgg gatccccgag ctcaagcggg
     7501 tcgcggtcgt gttggacgac gaggtcgcgt tcgaggacac gttcgacgag gcgctcgccg
     7561 agctcttcgg cctggggacc cctgagccgc ccccggagcc gggagagccc gaggagccag
     7621 aagagccggg cgacccggag gggccggcgt cgccggagct catggagatc gtcgaggagg
     7681 cgggccgcgt ctacgaggcg gcccaggagg cgctcgcggc cggggacttc gaggagtacg
     7741 ggcgcctgat cgaggagctc ggcgagctgc tggaaagggc ggggtcgctg agcgggggag
     7801 ccgagaccgg ctcgggttag gtcttgcggc cgagcacctc gtcgacgagg gcccggacct
     7861 tggccgagtc ggcgttgcgg cacctgatgt tgcgctggag atagagcgtg ccgtccggct
     7921 cgtcaaagac ctgctcttgg tattccgcct cgatgcctct ctcggcgagc cgccggacga
     7981 tctcgtcgcc ggtgacgggg tcgagcccgt cacggccgat ccccgtcgtc atgttgcgcc
     8041 agtggacggt gtcgaggacg agcgcgacga gccacgcggc gacgacgccc gcggggagcg
     8101 cgatcttcgc cgggctcccc gcgatcaccg agagggcccc ggctgcgagc ccggccgcga
     8161 cgagcgtggc ccacccggcg aggttgagct cgacgctccg gaacccgccg agcgaacgcc
     8221 gctctagcag agggtcgaac atgcgtctac gacgcagctc gacggggctt ggtccgtcca
     8281 cggccggagc ctacgcttgg tagagtcgag gggcagggtg gagcagtctg gtagctcgcc
     8341 gggctcataa cccggaggtc gcgggttcaa atcccgcccc tgcaaccaag aggcccccgc
     8401 aagggggcct ttctcgtccc gtggcgctct agctcgcccg agctatctgc acgtcgagcg
     8461 tcaggatgtc ggcgtcggcg tcgagctcct cctcacccga ctcgattacg tcccgaattg
     8521 cctcgacggc aaaccgctcg gcgtccgccc tcgaatcgca gccggtgccg aggatggaga
     8581 gcgccggaac ggtatacccc cactggcgca cctcgtcgtc gtagaacagc tcgacgtgaa
     8641 gtctcatctc gtccattctg ctacaccatc cgcctgagct cctcgagcgt gtagccgaac
     8701 gaacgggcgg tcttggccat catgggccca ccaagatcag tgccgtggtg atgagtccag
     8761 cgcacccttc ggccgttctt ctcgagggtc acgtgcgacc cgcgccgccg aacctcgacc
     8821 cagccgtccc gcttcagcgc cgccaggacg cgggcggcct tgggcacgcg ggatcgtcgg
     8881 tgtcggctcg cacgccgaaa cgatcggacg agcgctcccg accccgtgtg gccggcgcca
     8941 ctgccgcagg gaaatccctc ctgccgagcc ggtcgatcct cggctagcct gggcccgtgc
     9001 ttcgtccgac cgttcgcgtc gccgtcgctg cggccctcgt gctcgcggcc agtcttccgg
     9061 cgctcggggc cggggcttcg accgaccccg agcgccccgc ctgctcccac ggctaccgcc
     9121 ggagccgcga ggtcgcccac ggccgcttcc tcggccgccg gtgggagctc gtcttcttcc
     9181 gggaccgaaa ggatcgcccg tgcctctccg acagttgggg gccgtacggc tcgatcttcc
     9241 gcttccgcgt gcgcgagcac cggcctcggc tcgggatcgt caacctcaac gcgccgacgc
     9301 cgcccgacgg tcccggcgcc tacgtcttcg aggcctacgt ccaggagcgg gtcgcgaggg
     9361 cgacgttcac gatcgacgga cggacacgtg acgtgcggat cgttcggtcg cctcgctgga
     9421 cgcggctccc gaagaacctc ttgatccatt tcgtcgggac ccgccggtac aaccgggacg
     9481 ccacgggacg cctccgggcg ttcgactccg agggccggct gctcgcgacg agggtcctcc
     9541 ggcgaggcca gttctccaag agcgccgagc cccagtaggc acgccgccgc cacccgccgt
     9601 cggagggatg acggcccctg cggagaaccc cccggccatg aagacgactc gttcgatccg
     9661 ccgcgccctc ggcgccctcg tggccgccgc cctcgtcgta cccctgtccc aggctccggc
     9721 ctctgccaag gcggaggccc ccgacgagcc ccgcgtcgtc gtcgccgtga tcgacaccgg
     9781 cttcaacgcg ttccacgagt acttctacaa ggggggcccg ctctacggga acaagggccc
     9841 gtcgagcgtg acgccggccg ttctgaagga gttcggcatc ggcaaggacc agatcatccg
     9901 cgtcccccgg accggcgact tcggcaagga cttcgcgaag gtgcaggcga agttcgacgc
     9961 aatcgagaag ggcgagccgt actggttcgc cgggaccaac gtgatcggca tctccttcct
    10021 cggcgacaag cagcgccttc gccccgacgg caacgccagc agccacggca tcggcacggc
    10081 ggcgtcggtc ctcacggcga accccgaggc catcgtcgtc gcggtcgagg actggaacgt
    10141 cgacgccgcc gagaagtggg cgttcacgca cccggcggta gacatcgtca gcacgagcta
    10201 cggcccggcc accagctacc cgacgttcac gaacctgtcg ttcagctaca cgggcgtcgt
    10261 cgagaacggc aaggtccact tcggcgccgc tgcgaacgac ccgacgtacg ccggcatcga
    10321 cgagaccggc ggcccgtggt ggagcatcgg cgtcgccggc ttcgaggagg gcgcgagcgg
    10381 cggacgtcag ctcctgtccg gccaggtccc cgacttcgtc ggcgacttca cgcaggacct
    10441 cccgtactgc cgcagctgcg agcagggtct ccagtccgtc ggcggcacga gcttcgcgac
    10501 gcctcgcagc gcggggacgt tctcgaaggt cctgctcgag acgcggcggg ccgcgggcca
    10561 cgacggcggg atcgtcaagc ccggcaaggg tgcgccgctg ctcgtcgacg ggaagatgaa
    10621 gctgacgaac tgggacatgc gccgtgccct cgaagaggcg gcctactacc cgacgaccgg
    10681 cgagtacggg accgagggcg gtgccgaccc gacgtcggtc cccgtcaacg acgcggcccc
    10741 gtgggtgcag accggctggg gcctgatctc gccggcgagg cagttcgagg tcgtgaagga
    10801 gatgctcgcc cacctgggcg tcgcggggaa gccgacccgc acgaagtcgc aggaggcgtg
    10861 cgacttcatg accgcgaaca tggaggcccg ccgcgcgtac tggaacgacc tcgccatgaa
    10921 cggcgagtcg ttcgggatcg gcgacgaccc ctacgtctac tgctgagagc ggacgccggg
    10981 ctcagccctg agtctcggcc agcacgatca ggcggtcgtc ggcccgcgcc tcgaacgtcc
    11041 ggcccttcgg cgggttgatc gtcaccttcc gtccgtcgcc gccggcccac gcctggtaac
    11101 cgagcgcgac ctcgccgcgg gccgcagccg acgcggtcag gtccccgaac gagacctgcg
    11161 tcgccggtgg cgcgtagagg cttgcgggct tgaggtagat ctcggccccc gcttcgtcga
    11221 gcagatcgtc gaacacgggc gcgagctcgc ggttctccga cagctgggcc atgatcagcg
    11281 cggtcagccg ctcgctcacg atgaactcgt cggtcgtccc cgaagggctg aggtcgacgt
    11341 cgcgctcgtc gaggagctcg gcgacgacgc tcgtcggtgc tcctgcctgg tcgatggcgg
    11401 cgcgcaggtg caggagcgtg agcaggacac gcgcgtcggc ctgcgcctcg ctcagccgct
    11461 ccttgtagca gaggacgatc acgtgctccg gcgcttgcga ccccatgatc gtgcgcagcg
    11521 cgtcgggatc gagcgcctcg gtgtgcaccg tcgcgctgag gttgcgggtc tcgggcacgt
    11581 cgacctcttc ggcgggcacc acgaccgggt cgacgtggac tgacagcacg ctgccgggcg
    11641 cgacgtagtt gtcgagctcg cgcacgatct cgggcgccat gcggttccag ccgatcatca
    11701 ggatgcgctc ggcctcggtg cgcgccgcaa ccgcttcgcc tcgggaggcg ctcccggcgc
    11761 ccgcccccgt gaagacgatc gcgtcgtcgt cggacgcgat cgcgacgagc cggtctcctt
    11821 cggagatcgg cgtgtcggcg ctggggttca gcgcgacgcg gccgtcgccg aacctcagcc
    11881 ccagcgggac ggcctgctcg aacgacgtca gcgcggcgcc gaacgtcgag cccgcgagct
    11941 tccccgccgg cgcgaagtag atctcgtcac cggcgaagtc gaacagctcc tggtagacgt
    12001 cgctgaggcc gggctgccgg cacacctgcg cggtgacgcg ggcgatgatg ctcgtcgaac
    12061 ggacgacgct cacggccccc gccgtcccgt cgcgcagcgc cgccgcggtg aacgcgtcgg
    12121 agacctcggc gaccaccggg acgtcgcccg cgagcttcgc cgtgcggatc agcgcgagcg
    12181 cggtcttcac gacctccgcg tcgccgtcgg cgtggccggg gttcagcacg acgatcgact
    12241 tcgccgcctc cggccgcgcg atcgcgacgt cgaggggctc gaaggggact ccgttgcggc
    12301 acacgatgcg cgtcccccgg cgctccttca cgcgagcgtc cagcagccgc tccatctcgt
    12361 ccttgtccat gtcggcgacg acgacgaccg caccgccgcc ctggctctcg ttcgcgaccg
    12421 tcagctcgga gatgatcggg aagaccttcg gcgaccatcc gaggatcagc gtgtgaccgg
    12481 tctcgaggac gggcgaccgg ccccggcgga gctccgcgac gcggtccgag atcccgttcg
    12541 cgaggaggcc gatcagcgag ctgacgatca ggatcccgcc gatggtcgcg gccagcgacg
    12601 cgaccctcag aggccacccg acgtcgccgc ccatggtccc ggggtcgagc gttcgcatca
    12661 gcgtcgacca cagcgcctcg ccgaacgaca gctcggcccc ctcggcgcgc acctcggcga
    12721 tcgcggcggc cgtggcgatc acgaggatca gccctaccgt cgcgagcgtc agccacagga
    12781 ccatcgcggc ggtcccggac gagagcgtgt tgtcgaacca gtagcggaga cgttgccgcg
    12841 cggtaggacg ggggggcgtc ggcacgccgg gcaggctatc ccacgcccgc gtcgataatc
    12901 ggacccgtgg acggggagag gttcgccgag gtagaccgct acctcgccgc gctgctggcc
    12961 ccgccggacg gcgagctgac cggagcgctg gaggcatccg ccgcggccgg gctcccgccg
    13021 atcgaggtca ctccgaccga gggcaagctc ctccacctgc tggcccgcct ctccggcgcc
    13081 cgctcgatcc tcgaggtggg gacgctcgcc gcctacagca cgatctggct ggcgcgggcg
    13141 ctcccggccg gcggccggct cgtgacgatc gaggccgacc cggtacacgc cgaggtcgcc
    13201 cggcgcaacg tcgggcgggc ggggctggac ggcgtggtcg aggtacgtga gggggccgcg
    13261 ctcgacgtcc tgccggacct cgtccgtgac ggcgccggcc ccttcggctt cgtgttcctg
    13321 gatgcggaca aaccgaacaa tcccgcctac ctcgagtggt cgctgaggct cacggccccc
    13381 ggcagcgtga tcgtcgccga caacgtcgtc agagggggag ccgtggcctc ggcgaccgac
    13441 gaccccagcg ccgccggcgc ccgccgtttc ctcgagatgg tggcggccga gccccgcctc
    13501 gaagggacgg ccgtccagac ggtcgggacc aagggccacg acgggttcgc gctcgcgctc
    13561 cgcgtgtcct gatcgaccca tctgcgcagg tcgaaatagc tacttcgggc cactcaactc
    13621 ctcccggtcc agggccgatg ccacagtgag gacgcgggac atatcggggg agacggggcg
    13681 gcgagccttc ccggccgagc ttgcgtcgta ggtcgtgtcg gaggggtcga gtgtgacaga
    13741 cgtcatcgcg tcggagcgaa ggatcggaga ccggttccgg ttcctgaccg gcgagcagaa
    13801 ggtgtggatg ttcaacgcct tgctcgccgg ctgcgccgcg gcggtctact tctccttcgt
    13861 cgtcgacatc ccgacgccca gcgcgccgtt ccacctctcg tggtgggtgc tcgcgccgct
    13921 gttcgcgttc tccgagatct gcgtcatcca cgtccagctc cgccgcgagg tcctgtcgta
    13981 ctcgctgaac gagatcccgc tcatcctcgg cctcttcttc gcgtctccgc ccgacctcgt
    14041 cctcggcgtc ctcgccggcg gcggggccgc cctcgtcctc catcgccggc aagcgatgct
    14101 gcgcctcacg ttcaacctgg cccacttctg gcttacgagc tgcatcgcga tcctgatctt
    14161 ccactccgtg gtcggcaacc atgcgcacac cggccccgcc gggtggctcg gcgccttcct
    14221 cggaggcttc accgccgaca cgatcgccgc gttctgcgtc gtgctcgtga tgtcgctcgc
    14281 cgaaggacgc cggcagagcc tcaaggccct gatcggtccg ggatggatca tcacgctcac
    14341 gaacgcatgc gtcgcgctgg cggccgtcac cgtcctctgg gtcgaccccg tctctgcatg
    14401 gctcctcgcg gtcccggccg cgctgctgtt cctggcctac cgcggctaca acgcccagcg
    14461 gcacaaacgg cagagcctcg agctcctcta cgagtcgacg cgcatcctcc agcagtcgct
    14521 ggaggcgaac tccgtcgtcg acaccgtcct cgaacaagcc cgtcagatgc tccgagcgga
    14581 ctacgccgag ataacgcggt tcccgacggt caaggggggc cgggccttca gggcctcgct
    14641 cggcccgggc gtcaggagag agccactgcg gtcgatcaac ctggatccga ccgagggcgt
    14701 gtgggcgagg gtcgctgccg aggggcagac gatcctcgta gcgcgtccga tcgagaacga
    14761 gaagctgcgc acccacttcc acgagcgcgg gatccgcgac gcgatcgtcg ccccgctcca
    14821 cggggccgaa gggatcatgg ggacgctgct cgtcgggaac aggctgcgcg acgacctcgc
    14881 gacgttcgac tccgacgacc tgaagctcgt cgagacgctc gccaaccacg ccagcatctc
    14941 gctcgagaac ggacgtctgg tcgagagcct caagcgccag gccgcggaga acgagttcca
    15001 ggcccagcac gacgcactca ccgggctgtt caaccgcacg atgttccaca accgggtgtc
    15061 gcaggcgatc gcgcaggcgg gctcggacat ggtcggcgtc ctcctgatgg acctcgaccg
    15121 cttcaaagag atcaacgaca cgctcggcca ccccacgggc gacagcctgc tgaaggagac
    15181 gtcgttccgt ctgcgccgca tcctcggccc cgacgtgacg atcgcccgcc tcggcggtga
    15241 cgagttcgcc gtcctgctcc ctcgcctgcg ctctttgatc ggggcgaccg acatggcggg
    15301 gaacctcgtc tccgccctgc gggagcccta cgagctccag gacctctcgc tcgacgtcgg
    15361 tgcgagcatc gggatctcgc tgttcccgca gcacgggacc gacgccgaga cgctgatgca
    15421 gcgagccgac gtcgccatgt acatcgcgaa ggcgtcacac agcgggttcg agatctacga
    15481 cagtgacaag gaccagtaca gcccgaaccg gctcgcgctc gtcgccgagc tgcgccgtgc
    15541 gatcgaggag cgccagctcg tcgtctggta ccagccgaag gtcgacctcg ggtccggcag
    15601 cctgatcggc gccgaggccc tcgtgcggtg gaagcacccg cgccacggca tgatgccgcc
    15661 ggacgagttc atcggcctcg ccgagcacac cggtctcatc ggcccgatga cgacctacgt
    15721 gctcgaagag gccctgcagc agtgcctcgc gtggcggtgc tccggcttcc cggacttcaa
    15781 ggtcgcggtg aacctctcgg tcaagaacct catggacgtc gacctcccga gccaggtcag
    15841 cgcgctgttg aacaagagcc agctcgtccc cgacgccctc cagctcgaga tcacggagag
    15901 cagcatcatg gaggacccgg aacgcgcgat cgaggtcctc acgaggctcc acgagatggg
    15961 gatcggcatc gccatcgacg acttcggcac gggctattcg tcgctcgcgt acctgcgcag
    16021 gctccccgtc gacgagatga agatcgaccg cagcttcgtg cgcggcatga tgtcgtcgga
    16081 gaacgacgcg gtgatcgtcc gctcggtcat cgacctcggc cgcaacctgg gcctggtcgt
    16141 ggtcgccgag gggatcgagg accgcgagtc gatggagcgc ttggaggcgc tgggctgcac
    16201 ggtcggccag ggctaccaca tagccaggcc gatggacgtg gagggcttcg actcctgggc
    16261 ggcgagccgc cacgcggccg aagcggcggc ccccccggcc ccgccacccc cgtcggtagt
    16321 gccgttcccg ccccgcctgc agagcctcta gaccttctca cccctgcttc gcgatggccc
    16381 tggtcccgga aagcgggact gcgcccaact cggacggccc ccggcgcact acgagtagcg
    16441 cgttatgtag cccctcgttc ggaaggtcga cagcatcttc gccaaaaaga gcgtcgccgc
    16501 caggcctagc gcgacggccc cgccggccgc cagggccagc tcgcgccatt ccatgccctg
    16561 cccgtccagg agcgagcgca tcgccgtgaa cgcgtgcgtt gtcggcagca gcctcgctat
    16621 cggctggacc gcgcccggca gggccggcac cgggtagaag acacccgata gcggcatgac
    16681 gaggaacagg atcccccacg ccagggcctc ggcgccgctg ccgaacctca gcacgagcgc
    16741 gatcacgaac agcgcgatcg cccatcccac ggcgagcagg accgccgcga tcgggatgag
    16801 ccccaacccc acgtcggtga tgtcgaacgc gaagaagccg agagctgcga gggcgacgag
    16861 gccgacgccg agcgcgagct tcacgaggcc gaacagcgcg acgccggccg cgtactcgac
    16921 ctccttgagc ggcgtcacca tcaggttgag cagattgcgc gaccacgtct cttcgaggaa
    16981 gcccgtcgag agcgcgatct ggctctggta cacgacgtgc cacaggacga tgccggcgag
    17041 caggtagccg taggcagcgg cctcgccgcc ttcggcctcc gacaggaaga ctccgagcga
    17101 gccgaacagc aacacgtcga ccaacggcca gatcgtgatg tcgtagagcc ggtgggggct
    17161 gcgacgcagg acgtacgcgt gacgcttggc gatggacttg acgcgcagcc agctcacagg
    17221 tggtcggtct cctgggcgcg ggacgcgtcg cgggcgttcg acgcgaggtg gaggaagacg
    17281 tcctccaggt cgtcctggcc gaagcggccg gcgacctcct cgggcgagcc gtcggcgacg
    17341 atgcgtcccg cggacatgaa gatcacgcgg tcgcacaggc gcgcgacctc gaccatgttg
    17401 tgactcgtca cgaggagcgt ggtcccctcc ttctcgcaga ggtcgtgcag accctgacgg
    17461 acgcgctggg ccacgtcggg gtcgagggac gcggtgggct cgtccagcac gaggaggcgg
    17521 gggccgtgga gcgtcgcctt gatgatcccg acgagcgtcc gctgccccga cgacagctcg
    17581 ttgcccatga cgttggcgag gcgcgtgatc ccgaaccgtt cgagcccgag ctcgatgcgt
    17641 tcacgcgggt ccttcaaccc gtagagaccg ccgtagaggg cgaggaactc gcggacgcgc
    17701 aggcgctcgg cgagcggcat gtagcccgcg gcgaagccga cgcgctccat cgcctcgctg
    17761 cggtgctccg gcagccgcag cccggctacc tcgacgtggc cctcgtcggg cgagatgacg
    17821 ccgagcagca tgaacaacgt cgtggtcttg cccgcgccgt tggggccgag gagcgcgacg
    17881 cgctgtcccg ccgagacggt gaggtcgacg ccgtcgacgg ccttggtcgc gtggaatgtc
    17941 ttggtgagcc cggtagcccg gatgacgtgc tccatggcga ccgactatct cagaccgttg
    18001 ccgacaggcg tgacgcctcg gcccgtgccg gacactcaag ggatatgagg cgcgacgaac
    18061 gtcgggaagc caggttccgt gcgctgtacg gagagcatct cgacgccgtc gcccgttacg
    18121 ccgcgcggcg cgtggatcac gccgaggtgc acgacgtggt ctccgaggct ttcctcgtcg
    18181 cgtggcgtcg tctcgaagac gtgccggcgg atccgctccc gtggctgttc gcgacggcgc
    18241 gcggcgtgat ctcgaacagg cgcaggtcct cgctccggcg gcgtgccctc cgcgacaagc
    18301 tcgccctaca ggtggcgacg c
//
LOCUS       DASQDO010000026         5086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_616063, whole genome shotgun sequence.
ACCESSION   DASQDO010000026 DASQDO010000000
VERSION     DASQDO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616063"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VEV43_00840"
     CDS             <1..1602
                     /locus_tag="VEV43_00840"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HYP22099.1"
                     /translation="ALDAARNAGAQVFTAPELAISGYPPEDLLLKRSFLDAQVAGLHE
                     VAAHTGELLAFVGFAQPTSRSLYNAAAICQHGEVLAVYRKHLLPNYGVFDELRYFDPG
                     RGHTLVETPDGVIGVCVCEDAWSPRGPVVSQGDAGAQIVVNVNASPWDRHKLGLRTEM
                     LAERARRARTSIVYLNTVGGQDELVFDGGSLVLDADGEVVARLPQFEETVAIVDVPLG
                     ETKPTRHPSVRRIARTLRPASGAAPPLEGRELSPEEAVYTALKLGLRDYAVKNGFRKV
                     VVGLSGGIDSSLVATIAADALGPGAVLGVSMPSQFSSGHSVEDAKELSANLGIEMLEI
                     PIRTTHHAYLDALKDTFGDAEPGLAEENLQARIRGNLLMAISNRYGHLVIATGNKSEM
                     ACGYATLYGDMAGGFALLKDVFKTEVYALALYRNGLGHVIPDNVLTKPPSAELRPGQK
                     DSDSLPPYDVLDPILELYVEGDADVDDVVAAGHDRATVERVVTLVDRAEYKRRQAPPG
                     PKVTTKAFGRDRRLPITNHWRDRTD"
     gene            1618..2313
                     /locus_tag="VEV43_00845"
     CDS             1618..2313
                     /locus_tag="VEV43_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP22100.1"
                     /translation="MAITVLLLSDTLEDVGELLPSFPAAPYRVVSRGLKETLPDDLIP
                     IHPDLLLVDATGDASVAAEATRRLARAWEVGLPPSLAVVDEAAIDGFRFEAGADDFVV
                     TSASQAEIAARLSLLTRRAGHGDEATVLKVGDLTVNPENYQVYVRGRPLDLTYKEFEL
                     LKFLAQRPGRVCDRDLLLREVWGFDYYGGTRTVDVHVRRLRAKLGAEHEALIETIRNV
                     GYRLVPRPRERRP"
     gene            2310..2627
                     /locus_tag="VEV43_00850"
     CDS             2310..2627
                     /locus_tag="VEV43_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HYP22101.1"
                     /translation="MTTVVGGVLAILFVDPPWRWLIIGALLLTDVVEIVIWLRWRNKK
                     SITGIESMVGQGGRATTTLDPDGQVRVRGQLWSAHATERVEVGDDVTVTAIDGLKLEV
                     TPR"
     gene            complement(2682..3023)
                     /locus_tag="VEV43_00855"
     CDS             complement(2682..3023)
                     /locus_tag="VEV43_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22102.1"
                     /translation="MSRFRLSTIAVVLGLLGGLALTPTASREGRAQHEGAALVLYASD
                     DLQEEGFSLNDFSRELERRVPDLGASTPSEIKRAARKAAGNFESEVDRKIKIRVGYSC
                     CPHEITLSVSW"
     gene            3832..3903
                     /locus_tag="VEV43_00860"
     tRNA            3832..3903
                     /locus_tag="VEV43_00860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3865..3867,aa:Lys,seq:ttt)
     gene            complement(3983..4636)
                     /locus_tag="VEV43_00865"
     CDS             complement(3983..4636)
                     /locus_tag="VEV43_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP22103.1"
                     /translation="MIRVVLVDDQDLVRSGFRVLLGSSGEVEVVGEAEDGVRAVEVVA
                     QVKPDVVLMDVRMPRMDGIEATRRIRDSSDAKVLILTTFDLDEYVHEALRAGASGFLL
                     KDARPEELFDAIRVVHAGDALLAPSITRRLIEEFVRRAPPAPSTATTLESLTERELEV
                     LRLVARGLSNAEISASLVVSPATTKTHVSRILTKLGLRDRAQLVMVAYETGLATPGA"
     gene            complement(4633..>5086)
                     /locus_tag="VEV43_00870"
     CDS             complement(4633..>5086)
                     /locus_tag="VEV43_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP22104.1"
                     /translation="LAETRRVLGLLRAPEQAPAIEPAPSLAGLEELVAGVRGAGVDVQ
                     LHRSGDFADVTPGQQLALYRIVQEALTNVIKHSGAGKARVVLTRSGKEVRLEVVDDGR
                     GSRGSPGQGAGLRGMKERVNAYGGRFDASDLAEGGFKVTVTIPVGAGS"
BASE COUNT          780 a         1763 c         1761 g          782 t
ORIGIN      
        1 gcgctcgacg ccgcccggaa cgcgggcgcg caggtcttca ccgcccccga gctcgcgatc
       61 agcggctacc cgcccgagga cctcctgctg aagcgttcgt tcctcgacgc gcaggtcgcg
      121 ggcctgcacg aggtggccgc gcacacgggc gagctgctcg cgttcgtggg gttcgcgcag
      181 ccgacctcgc gctcgctcta caacgccgcc gcgatctgcc agcacggcga ggtcctcgcc
      241 gtctaccgca agcacctcct gccgaactac ggggtgttcg acgagctccg gtacttcgac
      301 ccgggccgcg gccacacctt ggtcgagacc cccgacggcg tcatcggagt gtgtgtgtgc
      361 gaggacgcgt ggagcccgcg cggccccgtc gtctcgcaag gtgacgccgg cgcgcagatc
      421 gtcgtgaacg tcaacgcgtc gccgtgggac agacacaagc tcgggctccg gacggagatg
      481 ctcgccgaga gggcccggcg cgcgcggacg tcgatcgtct acctgaacac ggtagggggg
      541 caggacgagc tcgtgttcga cggtggttcg ctcgtcctcg acgcggacgg cgaggtcgtg
      601 gcgcggctcc cgcagttcga ggagacggtc gcgatcgtcg acgtgccgct cggcgagacg
      661 aagccgacgc gtcatccgtc cgtgcggcgc atagcgagaa cgctgcgtcc cgcgtcgggg
      721 gccgcgccgc cgctcgaggg gcgcgagctc tcgccggagg aagcggtcta caccgcgctg
      781 aagctggggc tgcgcgacta cgccgtcaag aacgggttcc gcaaggtcgt cgtcgggctg
      841 tcgggaggga tcgactcgtc gctggtggcg acgattgccg cggacgcgct cggacccggc
      901 gccgtgctcg gcgtctcgat gccgtcgcag ttctcgtccg ggcactccgt cgaggacgcg
      961 aaggagctgt cggcgaacct cgggatcgag atgctcgaga tcccgatacg gacgactcat
     1021 cacgcgtatc tcgacgctct caaggacacg ttcggcgacg cggagccggg gctcgccgag
     1081 gagaacctcc aggcgcgcat ccgcggcaac ctgctgatgg cgatctcgaa ccgctacggc
     1141 cacctcgtga tcgcgaccgg caacaagtcg gagatggcgt gcggctatgc cacgctctac
     1201 ggcgacatgg ccggcggctt cgcgctgctc aaggacgtgt tcaagaccga ggtctacgcg
     1261 ctcgcgctct accgcaacgg gctcggccac gtgatcccgg acaacgtgtt gacgaagccc
     1321 ccgtcggccg agctgcggcc cggccagaag gactccgact cgctcccgcc ctacgacgtg
     1381 ctcgacccga tcctcgagct gtacgtcgag ggggacgccg acgtggacga cgtggtcgcc
     1441 gccggacacg accgggcgac ggtcgagcgg gtcgtcacgc tcgtcgaccg ggccgagtac
     1501 aagcgccggc aggcgccccc ggggccgaag gtcacgacga aggcgttcgg gcgggaccgc
     1561 cggctcccga tcacgaacca ttggagagac cggacggatt agtccaccat tgaggggatg
     1621 gcgatcacgg tcctgctcct gtcggacacg ctcgaagacg tcggcgagct gctgccgtcc
     1681 tttcccgccg cgccgtaccg cgtggtctcg cgggggctga aggagacgct gcccgacgac
     1741 ctcatcccga tccatcccga cctcctcctc gtcgacgcga cgggggacgc gagcgttgcg
     1801 gcggaggcga cgcggcggct ggcgcgcgcg tgggaggtcg ggctgccgcc gagcctcgcg
     1861 gtcgtcgacg aagccgcgat cgacggcttc aggttcgagg ccggtgccga cgacttcgtc
     1921 gtgacctcgg cgtcgcaggc ggagatcgcg gcacggctct cgctgttgac acggcgcgcc
     1981 gggcacggcg acgaggcgac cgtgctgaag gtcggcgacc tcaccgtcaa ccccgagaac
     2041 taccaggtgt acgtgcgggg ccggccgcta gacctcacgt acaaggagtt cgagctgctg
     2101 aagttcctcg cgcagaggcc gggccgcgtc tgcgaccgcg acctgttgct gcgggaggtg
     2161 tggggcttcg actactacgg agggacgagg accgtggacg tacacgtgag gcggttgcgc
     2221 gcgaagctgg gggccgagca cgaggcgttg atcgagacga tccgcaacgt cgggtaccgg
     2281 ctcgtcccga ggccgcggga gcgacggccg tgacgacagt cgtcgggggc gtcctcgcga
     2341 tcctgttcgt cgacccgccg tggcggtggt tgatcatcgg cgcgctgctg ctgaccgacg
     2401 tcgtcgagat agtcatctgg ctgcggtggc gcaacaagaa gtcgatcacc ggaatcgagt
     2461 cgatggtcgg ccaggggggc cgcgcgacga cgacgctgga cccggacggt caggtgcggg
     2521 tgcggggtca gctatggagc gcgcacgcga ccgagcgggt cgaggtaggc gacgacgtga
     2581 cggtgacggc gatagacggc ctgaagctgg aggtaacgcc gcgctagccg gcgcagaacg
     2641 tgccgccggc gagcgacgtg ccactacttc cgccttctca gctaccagct caccgaaagg
     2701 gtgatctcgt gcgggcaaca gctgtacccg acccggatct tgatctttct gtcgacctcg
     2761 ctctcgaagt tcccggcggc cttcctggct gctcgcttga tctcagaggg cgtcgacgcg
     2821 ccaagatccg ggactcgtcg ctcgagctct ctgctgaagt cgttcagcga gaaaccctcc
     2881 tcctgaaggt cgtcgctcgc gtacaacacg agggcggcac cctcgtgttg cgcccgccct
     2941 tcgcgcgacg ccgtcggcgt cagggctagt cctccgagga gtccaagaac gaccgcgatc
     3001 gtcgacaacc tgaatctgga cacggtgcct cctctattgc gtcggatgag ttgcatcggg
     3061 aaaggctttg accgggggcg accgggcggg cggtatcgac cgatcgtcgc gacgacctcg
     3121 cagacgccgt agaccgcgtg agaccaacgc gttgacggcc cgctcggaca acccgaggat
     3181 ctctgcggtc tcgcgctctg atcgatcctc gagatagcgg agagcgaccg ccgtcctttg
     3241 ccggaccggc aactcgagga ggcggcgtcg cagatggtcg tggacctcaa cgtcgggcag
     3301 gttctcagcg gagggcgcgt ccgggccgag gagggtacgg actcgtgctt cgaggcgatt
     3361 tcgccgaaag tgccccacgg ctaggttctc gacggtgcgg agcagataag gcaacggcga
     3421 ctcgacgtcg cctacggggc gcgctatcag cagcaggaag gcctcttgtg cgaggtcgcg
     3481 tgacaactct tcgtcgtgcg tgatcaggaa tgccagccgg atggcgcgag gcgcggcgcg
     3541 tcggtagagc tcctcgaacc cttctatgcc tcctgtttcc cgagtcatca tgcgtcccga
     3601 cccgcccgcg aaccccgccc actgccccta cctgacgcgt cagacgacct cctcgcttcg
     3661 cgccgagttc agtggccgga cgagatcatt gcggggactc gtagccgggg tcggaacggc
     3721 ccggttcgtc gcgcctgagt ggcgcggtaa cgagggaacg gtcgcggcgc gcctgttcgt
     3781 cctgcaccag gtgccgcccg acctgggaga atccccgcta gagaccatca agggccccta
     3841 gctcatcagg tagagctccg gacttttaat ccggcgggca gggttcgagt ccctgggggc
     3901 ccacgggcga cgggagcttg cccagaccgg tccgccctgc ccctggcgac ctctggacac
     3961 tgcggtgatc cccggagcga gctcaggcgc cgggcgtcgc caggcccgtc tcgtaggcga
     4021 ccatcaccag ctgggcccgg tcgcgtaggc ccagcttcgt caggatccgg ctcacgtggg
     4081 tcttcgtcgt cgcggggctc acgaccaggc tggcggagat ctccgcgttc gacaggccgc
     4141 gggctaccag tcgcagcacc tcgagctccc gttcggtcag cgactccagc gtcgtcgcag
     4201 tcgagggcgc gggcggcgca cgtctaacga actcctcgat cagccgccgc gtgatcgagg
     4261 gagccagcag cgcgtcgccg gcgtgcacga ccctgatcgc gtcgaacagc tcctcgggcc
     4321 gcgcgtcctt cagcaggaag ccgctcgctc cggccctcag tgcctcgtgc acgtactcgt
     4381 cgaggtcgaa cgtcgtgagg atcagcacct tcgcgtcgga cgagtcgcgg atcctcctcg
     4441 tcgcttcgat cccgtccatg cgcggcatgc gcacgtccat cagcacgacg tccggcttga
     4501 cctgcgcgac cacctctacg gctctcacgc cgtcctcggc ctcgcccacg acctcgacct
     4561 ccccggagct gccgagcagg acgcggaatc cggaacgcac caggtcctgg tcgtcgacga
     4621 gaacgacgcg gatcaagacc cggcccccac ggggatcgtc acggtcacct tgaacccgcc
     4681 ctcggcgaga tcgctcgcgt cgaatcggcc gccgtacgcg ttgacgcgtt ccttcatccc
     4741 gcgcaggccc gcgccttgcc cgggcgaccc gcgcgatcct ctgccgtcgt cgacgacctc
     4801 gaggcggacc tctttccccg accgcgtcag cacgacgcgg gccttgccgg ctcccgagtg
     4861 cttgatcacg ttggtcagag cctcctgcac gatccggtag agcgcgagct gctgacccgg
     4921 cgtgacgtcg gcgaagtcac ccgagcgatg cagctgcacg tccacgcccg cgccgcgtac
     4981 tccggcgacc agttcctcca gcccggcgag cgacggcgcc ggctcgatcg ccggcgcctg
     5041 ctcgggggcg cgcaggagcc ccagcacccg ccgcgtctcc gccagg
//
LOCUS       DASQDO010000027         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_861353, whole genome shotgun sequence.
ACCESSION   DASQDO010000027 DASQDO010000000
VERSION     DASQDO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861353"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VEV43_00875"
     CDS             complement(<1..140)
                     /locus_tag="VEV43_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP22105.1"
                     /translation="METGLNGKGVLVTGGAGGIGSALCRTFAGEGAKVGIHYNTSSER
                     AE"
     gene            165..1145
                     /locus_tag="VEV43_00880"
     CDS             165..1145
                     /locus_tag="VEV43_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HYP22106.1"
                     /translation="MKDVGIVGMPYSGKSTLFQALTSAGSAGGRSNQAVVDVPDSRLD
                     VLAGIEKSKKVVHAKVRFVDVPGGLTAQGIAEFRNTDALCMVVRAFGPDANPKSELAS
                     LEAEMVLADLTNVESGLEKAKKKARGSAEGKVEAAVLEKAHEQLNAERPLRDTAWDDA
                     ERKVLRGFGPLTMKPWIVVANTEEAGSLPEGLPEDAFAMSAALEAEVAGMDEAEAAEL
                     LTGFGVEEPGLTKVIGACYRALDLITFLTTGEDETRAWEVRKGAKAPEAAGAIHSDLE
                     RGFIRAEVVSYDELVEEGSWDAAKSAGKLRVEGKDYVVVEGDVLNIRFAV"
     gene            1223..1702
                     /locus_tag="VEV43_00885"
     CDS             1223..1702
                     /locus_tag="VEV43_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22107.1"
                     /translation="MRARLLTLLTLVTVVATAAIGVAAQPRREMLLYTATDDNFTVAA
                     PQEYQVVGTGDPEANYDFEIRKGDKAVSVMILDDSERPVSGIVTQWKPDGATEAGPAT
                     AMSYHAVTWQRFCTRTAAPVKLKPKLPVRILVMEGTCKDGTPSLPTTGDIVVDFHGG"
     gene            1788..1976
                     /locus_tag="VEV43_00890"
     CDS             1788..1976
                     /locus_tag="VEV43_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22108.1"
                     /translation="MPDREHYEGASVAESLRILADHHFETATRHISRYEYELIKRAAY
                     LLEVRGADENPPDLTADT"
     gene            2125..>3708
                     /locus_tag="VEV43_00895"
     CDS             2125..>3708
                     /locus_tag="VEV43_00895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYP22109.1"
                     /translation="MSSTRFGAACLAGLLFSSALTGLGPAAALEPVAQLDTGARHVPG
                     EVVVRYASGAEPAELRDQYDLDKVESLGPRQTELVRTEGSVAETVNELSADPGVVFAE
                     PNYVYRASALPNDPFFPQLWGLHQPSDVDMDAPQAWDVTTGSPSVTVAVVDSGVAIDH
                     PELAPNVWRNPGESGGGRETNGLDDDANGYVDDVQGWDWVGDDATPRDLHGHGTHVAA
                     TIAAAGDNSTGITGVAMNARVMALRALNSNGAGTTAELTSAFTYAARNGAKVVNVSLG
                     GEGYSLSVLNAIAAAPDTLFVVAAGNGGVNNDLASSYPCNYPAPNIVCVAAMDSSDRL
                     ASFSNYGATTVDLAAPGVGILSVQPAFETKFYEDFTGGPGARWTTGGTNGTWGPEPDG
                     SVSDSPGTTYLDGADHWIATAAPADLSGGIDCLLRYSLKLDVEQGNDVLMIEASRDGS
                     TWETLSGWTGSTDGATDRMTEPLFDYDGAPSLHLRFRLVSNGSVTGDGATVDTVSVRC
                     SSPTYTGSELLYAGGTSMAA"
BASE COUNT          606 a         1263 c         1308 g          531 t
ORIGIN      
        1 ttctcggcgc gctccgagct cgtgttgtag tggatgccga cctttgcgcc ctcgcccgcg
       61 aacgtgcggc acagcgcgct cccgatcccg ccggcgcccc cggtgacgag gacgcccttg
      121 ccgttcagcc ctgtctccat cgggggcacg atacgttgtc gcccatgaag gacgtgggca
      181 tcgtcgggat gccgtattcg gggaagtcga cgctgttcca ggcgctcacg agcgcgggct
      241 ccgcgggggg ccgctcgaac caagcggtgg tcgacgtgcc ggactcccgg ctcgacgtcc
      301 tcgcgggcat cgagaagtcc aagaaggtcg tccacgcgaa ggtccgcttc gtcgacgtgc
      361 ccgggggcct gaccgcccag gggatcgccg agttccgcaa caccgacgcg ctgtgcatgg
      421 tcgtgcgcgc gttcggcccc gacgcgaacc cgaagtcgga gctcgcgtct ctcgaggccg
      481 agatggtcct cgccgacctg accaacgtcg agagtgggct cgagaaggcg aagaagaaag
      541 cgcgcggctc ggcggagggc aaggtcgagg ccgcggtcct ggagaaagcc cacgagcagc
      601 tgaacgccga acgcccgttg cgcgacaccg cgtgggacga cgcggaacgc aaggtgctgc
      661 gcggcttcgg cccgctgacg atgaagccgt ggatcgtcgt ggcgaacacc gaggaggccg
      721 gctcgttgcc ggagggcctg ccggaggacg cgttcgcgat gagcgccgcg ctcgaggccg
      781 aggtcgccgg catggacgag gccgaggcgg ccgagctcct gacggggttc ggggtcgagg
      841 agccgggcct gacgaaggtg atcggcgcgt gctaccgggc cctcgacctc atcacgttcc
      901 tgacgacggg cgaggacgag acgcgggcct gggaggtgcg caagggcgcg aaggcgccgg
      961 aggccgccgg agcgatccac agtgacctcg agagagggtt catccgtgcc gaagtcgtgt
     1021 catacgacga gctcgtggag gaagggtcgt gggacgcggc gaaatcagcg ggcaagctac
     1081 gcgtcgaggg caaggactac gtcgtcgtgg aaggcgacgt gttgaacatc cgcttcgcgg
     1141 tctgagttcg ttaccgcggc agcggggggc cgtagaccgt ggcgtagagg ttcggctcac
     1201 tcttgtctcg gaggtccttt cgatgcgcgc gaggttgttg acgctcctga ccctggtgac
     1261 cgtggtcgcg acggcggcga tcggcgtggc ggcccagccg cgccgcgaga tgcttctcta
     1321 cacggcgacg gacgacaact tcaccgtcgc ggcgccacag gagtatcaag tcgtcgggac
     1381 cggcgacccc gaggccaact acgacttcga gatccgcaag ggcgacaagg ccgtcagcgt
     1441 gatgatcctg gacgacagcg agcggccggt ctcggggatc gtcacgcagt ggaagccgga
     1501 cggggcgacc gaagcgggcc cggcgacggc gatgagctac cacgcggtga cgtggcagcg
     1561 cttctgcacg aggacggcag cgccggtaaa gctgaagccg aagctgccgg tgcgcatcct
     1621 ggtgatggag ggaacctgca aagacgggac gccgagcctc ccgacgacgg gcgacatagt
     1681 ggtggacttc cacggcggct gagccccgct ccccgcgctt cggttaaccg gccgcggcac
     1741 gtgggatata tctcgggtca aacctcgtgt catcgcagga ggtggaaatg cccgaccggg
     1801 agcactacga aggcgcgtcg gtcgccgaga gcctcaggat ccttgccgac catcacttcg
     1861 agaccgcgac gcgtcacata tcgcggtacg aatacgagct catcaaacgt gccgcatatc
     1921 tcctcgaagt ccggggcgcc gatgagaacc cgccggacct aactgccgac acctagtcgg
     1981 gctgcgcggg cgactgactg gtagggaggg gagccccgct aggaacaacc tcccggcccc
     2041 cctggttact tactgagcgg ctcaaagtcg ctaggccggg tgccgatgtt ccttgggggg
     2101 aaccgctctt tgctgggaga tctcttgtcg tccactcgat tcggcgcggc ttgtcttgcc
     2161 gggctccttt tctcgtccgc gctgaccggg ctcggcccgg ccgcggccct cgagcccgtc
     2221 gcccagctcg acaccggagc ccgccacgtc cccggcgagg tcgtcgtccg gtatgcctcc
     2281 ggcgccgagc ccgccgagct ccgcgaccag tacgacctcg acaaggtcga gagcctcggc
     2341 ccccggcaga ccgagctcgt ccgcaccgag ggcagcgtcg ccgagaccgt caacgagctg
     2401 agcgccgatc cgggcgtcgt cttcgccgag ccgaactacg tctaccgggc ctcggcgctc
     2461 cctaacgacc ccttcttccc ccagctgtgg ggtctccatc agccgagcga cgtcgacatg
     2521 gacgcaccgc aggcctggga cgtcacgacc ggaagcccgt cggtcaccgt cgcggtcgtc
     2581 gactccggcg tcgcgatcga ccatccggag ctcgcgccca acgtctggcg caatcccggc
     2641 gagagcgggg gcggccgcga gaccaacggg ctcgacgacg acgccaacgg ttacgtcgac
     2701 gacgtccagg gttgggactg ggtgggcgac gacgcgacgc cgcgggacct ccacggccac
     2761 ggcacccacg tcgccgcgac catcgccgcc gcgggcgaca actcgaccgg catcaccggc
     2821 gtagcgatga acgcgcgggt gatggcgctg cgtgcgctca acagcaacgg ggccggcacg
     2881 accgcagagc tcacgagcgc cttcacgtac gccgcgcgca acggagcgaa ggtcgtgaac
     2941 gtgagcctcg gcggcgaggg ctactcgctc tcggtcctca acgcgatcgc cgcggccccc
     3001 gacacgctgt tcgtggtcgc cgccggcaac ggtggggtga acaacgacct cgcttcgtcg
     3061 tacccgtgca actacccggc cccgaacatc gtgtgcgtcg cggcgatgga ctcgagcgac
     3121 cggctcgcga gcttctcgaa ctacggcgcc acgaccgtcg acctcgcggc ccccggcgtc
     3181 gggatcctca gcgtccagcc ggccttcgag acgaagttct acgaggactt caccggcggg
     3241 cccggcgcga ggtggaccac cggcggcacg aacgggacgt gggggccgga gcccgacggc
     3301 tcggtttccg acagccccgg cacgacgtat ctcgacggcg cggaccactg gatagcgact
     3361 gcggcgccgg ccgatctctc cggagggatc gactgcctgc tgcggtactc gctgaagctc
     3421 gacgtcgagc agggcaacga cgtgctcatg atcgaggcct cccgggacgg gtcgacgtgg
     3481 gagaccctga gcggatggac cggctcgacc gacggcgcca ccgaccgcat gaccgagccg
     3541 ctgttcgact acgacggtgc gccgtccctg cacctgcgct tccgcctcgt gagcaacggg
     3601 tcggtcaccg gcgacggcgc gaccgtcgac acggtgtcgg tgcggtgctc ctcgccgacg
     3661 tacaccggat cggagctcct ctacgccggc ggcacctcga tggctgcc
//
LOCUS       DASQDO010000028         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_494393, whole genome shotgun sequence.
ACCESSION   DASQDO010000028 DASQDO010000000
VERSION     DASQDO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494393"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2933
                     /locus_tag="VEV43_00900"
     CDS             <1..2933
                     /locus_tag="VEV43_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYP22110.1"
                     /translation="PGSRAGVLVEPGFDLIVAVLGVLKSGAAYVPVDPGYPEARIALT
                     FADAAVEVVITSGALDDLAPQGVRRVFVDGDAEAIAAQPGTDPERLAEPGDVAYVIYT
                     SGSTGRPKGVLVEHRSFANYVPAVAAAFGTTPEDRVLQFFSIGFDASLFDVSLALAGG
                     ATLCLGTRADLLPGTDLFTLMREERVTVALLPPSSLAAMPSNDLPDLRTLLVGGEVFP
                     GDLAQQWARQRRVFNAYGPTETTIASTWHEVPYAPAATLQSPPIGPPIANTQIYVLDR
                     NMEPVPVGIPGEMYIAGAGLARGYLNRPALTAERFVPNPFHGGRMYRTGDLARWRRDG
                     TIEFLGRVDRQVKVRGFRIELGEIENALLGHPGIREAVVDARGESVADRRLAAYYVPG
                     EPAPTVTELRAHLGTSLPDYMIPSAFVPLPDLPRTPNGKVDRAALPAPEAARPDLSTK
                     FEPPRTDTEKKLVEIWKAVLGLDRVGIFDNFFELGGDSILVIQVVARAAEEEIHLTAK
                     QMFQAQTIYDLSLLDEQQSATFKPEQGLVTGPVPLTPIQRWFFEKDPPEPHHFNQAMV
                     VDLDPGFTPEIVREAVGLILEQHDALRARYTRDGDEWRQFVGGLPDEVPFEHVDLRGL
                     EGDDEEAAYLAHAERIQTSIDLARGPLLRAAMFDRSPPHAPALLLVAHHLGIDAVSWP
                     ILIEDLHDVCRNLERRGAAKLPPKSTSFKWWAQKIEEYARSPLLIRELDYWLTVAPPD
                     VAALPVDATGGPNDVGSTDTIHAPTTVELTTALLHEVPAALGAQLMDVMVAALSLAVG
                     EWQGSPGVLMDLESHGRSDVFEGANLFATVGWFTSVYPFFTSLDPADDARANLEKARA
                     ALAAVPHKGFGYGPLRYMTAEGRRLAEMPQPEILFNYFGRIDAGYDDATFAPTFGETG
                     PTLSPRMPRGHLIEVNASVARNLLQVNWTYSRNRHRAETIEGLARSFERNLSAIVDLV
                     RSAP"
     gene            2930..3688
                     /locus_tag="VEV43_00905"
     CDS             2930..3688
                     /locus_tag="VEV43_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HYP22111.1"
                     /translation="MTADRWTVVPKPNADPRVRLFCFPYAGGSATTYRTWSDVLPPDV
                     EVQAIQLPGREWRLKEEPYRDVFPLVGELATVLKDRFDVPFAFFGHSLGALISFELAR
                     ELRRRGLSLPERLFLSAHRAPHLPKELPDIHDGPDEEFYEGLRKLEGTPDELLSNDEL
                     MELLLPVLRADFEIAETYRYPPEPPLDCQMSVFGGLGDEVTNRDILQPWDQHTTKDFK
                     LRMIPGSHFFVEESRDLILRAVFHDLMPVQRRHA"
     gene            3685..4353
                     /locus_tag="VEV43_00910"
     CDS             3685..4353
                     /locus_tag="VEV43_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HYP22112.1"
                     /translation="MIEVWRVDVRHVVDDFTGLLSPDEAERAGRFRFEPDRRSFVTTR
                     GILRTLVGAWLGVAPEAVAFGYGSAGKPEVEGVSFNASHAGGVALIALGSGRIGVDVE
                     SMRPDVEMRSLARRFFTRSENQALDRLPDEELALGFYGCWTCKEAFVKAVGEGLSFEL
                     DRVEVAVHPAPPGIVSVDGDEGAAAAWTLQAVEVGPGYAAAVVVDSPGAEVVVRDWPA
                     SAAA"
     gene            4350..>4671
                     /locus_tag="VEV43_00915"
     CDS             4350..>4671
                     /locus_tag="VEV43_00915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22113.1"
                     /translation="MNASAEHRSPEIEVFRLRRRHLNDVIAIEADVFARPWSTAYFMQ
                     ELSKPNRSYFVAEYDGEVIGFGGMMFTSENAAHALNVAVTPAVHRRQVGTRLLCVLMM
                     DAIAR"
BASE COUNT          683 a         1598 c         1677 g          713 t
ORIGIN      
        1 gtccggggag ccgcgccgga gtattggtcg agccgggctt cgacctgatc gtcgccgtgc
       61 tgggcgtgct gaagtcgggg gccgcgtacg tgccggtcga cccgggttac ccggaggccc
      121 gcatcgcgct gacgttcgcg gacgcggccg tggaggtcgt gatcaccagc ggcgcgctcg
      181 acgacctcgc gccacagggt gtgaggcggg tgttcgtcga cggagacgcc gaagcgatcg
      241 ccgcgcaacc cgggacggat cccgagcggc tcgcggagcc cggcgacgtc gcgtacgtca
      301 tctacacgtc gggttccacc ggccggccga agggggtgct cgtcgagcac cggagcttcg
      361 ccaactacgt ccctgcggtc gcggcggcct tcggcacgac tcccgaggac cgggtcctgc
      421 agttcttctc gatcgggttc gacgcgtcgc tcttcgacgt ctcgctcgcg ctcgccggcg
      481 gcgcgacgct ctgcctcggg acgcgggccg atctgttgcc ggggacggac ctgttcacgc
      541 tcatgcgaga ggaacgcgtg acggtcgcgc tcctgccccc gtcgtcgctc gcggcgatgc
      601 cgtcgaacga cctccccgac ctgcggacct tgctcgtcgg cggcgaggtc ttccccggcg
      661 acctcgcgca acagtgggcg cggcagcgga gggtcttcaa cgcgtacggg ccgaccgaga
      721 ccacgatcgc cagcacctgg cacgaggtcc cgtacgcgcc ggccgcgacc ctccagtcgc
      781 cgccgatcgg gccgccgata gcgaacacgc agatctacgt gctcgaccgc aacatggaac
      841 cggtgccggt agggatcccg ggcgagatgt acatagcggg ggccggtctg gcgcggggtt
      901 acctgaaccg tccggccctc acggctgagc ggttcgtgcc aaacccgttc catggcggcc
      961 ggatgtacag aacaggcgac ctcgcccggt ggcggcgcga cggcactatc gagttcctcg
     1021 gccgcgtcga ccggcaggtg aaggtgcgcg gcttccggat cgagctcggc gagatcgaga
     1081 acgcattgct cggtcatccc gggatccgcg aggcggtcgt cgacgcccgc ggcgagtcgg
     1141 tcgccgaccg gcgcctggcc gcgtactacg tgccgggcga gccggccccc accgtgaccg
     1201 agctccgcgc gcacctcggc acgtccttgc cggactacat gatcccgtcg gcgttcgtgc
     1261 cgctgccgga cctcccgcgg acgccgaacg gcaaggtcga ccgcgccgcg ctcccggccc
     1321 ccgaggccgc gcgcccggac ctgtcgacga agttcgagcc cccccggacc gacaccgaga
     1381 agaagctcgt cgagatctgg aaggcggtgc tcgggctcga ccgcgtcggc atcttcgaca
     1441 acttcttcga gctcggcggc gactcgatcc tcgtgatcca ggtcgtcgcg cgggccgcgg
     1501 aggaggagat ccacctcacc gcgaagcaga tgttccaggc gcagacgatc tacgacctgt
     1561 cgctgctcga cgagcagcag tcggcgacgt tcaagcccga gcaggggctc gtcaccgggc
     1621 ccgtcccgct cacgccgata cagaggtggt tcttcgagaa ggaccccccg gagccgcacc
     1681 acttcaacca ggcgatggtc gtcgacctcg accccggctt cacccccgag atcgtccgcg
     1741 aggcggtcgg gctgatcctc gagcagcacg acgcgctgcg ggcgcgctac acgcgcgacg
     1801 gcgacgagtg gcggcagttc gtcggcggcc tccccgacga ggtcccgttc gagcacgtgg
     1861 acctgcgcgg gctcgaaggt gacgacgagg aggcggcgta cctggcgcac gccgagcgca
     1921 tccagacgag catcgacctc gcacgcgggc cgctgctgcg cgcggcgatg ttcgaccgct
     1981 cgcctcccca cgccccggcg ctcttgctgg tcgctcacca cctcggcatc gacgcggtct
     2041 cgtggccgat cctcatcgag gacctgcacg acgtctgccg caacctcgag cggcgcggcg
     2101 ccgcgaagct cccgccgaag tcgacctcgt tcaagtggtg ggcgcagaag atcgaggagt
     2161 acgcacgctc gccgttgctg atccgcgagc tggactactg gctgacggtc gcccctccgg
     2221 acgtcgcggc gctgccggtg gacgcgaccg gcggcccgaa cgacgtcggc tccacggaca
     2281 cgatccatgc gccgacgacc gtcgagctca cgaccgccct gttgcacgag gtgccggccg
     2341 cgctcggcgc ccagctcatg gacgtgatgg tcgccgcgtt gtcgctcgcg gtaggggagt
     2401 ggcaggggag cccgggcgtg ctcatggacc tggagagcca cgggcgcagc gacgtcttcg
     2461 aaggagcgaa cctgttcgcg accgtcggct ggttcacgtc ggtgtatccg ttcttcacgt
     2521 cgctcgatcc cgccgacgac gcccgtgcga acctcgagaa ggcgagggcc gcgctcgctg
     2581 cggtcccgca caagggcttc ggctacggcc cgttgcggta catgacggcg gagggacgac
     2641 gcctcgccga gatgccgcag ccggagatcc tcttcaacta cttcgggcgc atcgacgcgg
     2701 ggtacgacga cgcaacgttc gcgccgacgt tcggcgagac cgggccgacg ctcagcccgc
     2761 gcatgccgcg cggccatctg atcgaggtca acgcctcggt cgcccgtaac ctcctgcagg
     2821 tgaactggac ctacagccgc aaccgccatc gcgccgagac gatcgagggg ctggctcgat
     2881 cgttcgagcg gaacctctcg gcgatcgtcg acctcgtccg gagcgcgccg tgacggccga
     2941 tcgctggacg gtcgtgccga agccgaacgc cgatccgagg gtgcggctct tctgcttccc
     3001 gtacgccggc ggaagcgcca cgacttaccg gacgtggagc gacgtgctgc cgccggacgt
     3061 cgaggtacag gcgatacagc ttcccgggcg cgagtggagg ctcaaggagg agccgtaccg
     3121 cgacgtgttc ccgctcgtcg gcgagctcgc gaccgtgctg aaggatcgct tcgacgtccc
     3181 gttcgcgttc ttcgggcaca gcctcggcgc cctcatctcg ttcgagctcg cacgtgagct
     3241 gcgccggcgc ggactgtctc ttcccgagcg gctgttcctg tcggcgcacc gggccccgca
     3301 cctccctaag gagctgccgg acatccacga cgggcccgac gaggagttct acgagggctt
     3361 gcggaagctg gaaggaacgc ccgacgagct gctctcgaac gacgagctga tggagctgct
     3421 gctgccggtg ctgcgcgcgg acttcgagat agccgagacg taccgctacc cgccggagcc
     3481 tccgctcgac tgccagatgt cggtgttcgg ggggctcgga gacgaggtga cgaaccgcga
     3541 catcctccag ccgtgggacc agcacacgac gaaggacttc aagctccgga tgatcccggg
     3601 gagtcacttc ttcgtggagg agtcgcgcga cctgatcctg cgggcggtgt tccacgacct
     3661 gatgccggtg cagcggaggc acgcgtgatc gaggtctggc gcgtggacgt ccgccacgtc
     3721 gtcgacgact tcacggggct cctgtcgccg gacgaggcag agcgtgcggg acggttccgc
     3781 ttcgagccgg accggcgttc gttcgtgacg acgcgcggaa tccttcggac gttggtcggc
     3841 gcgtggctcg gagtcgcacc cgaggcggtt gcgttcgggt acgggagcgc cggcaagccc
     3901 gaggtggagg gcgtgtcgtt caacgcgtcg cacgcgggtg gcgtcgcgct gatcgcgctg
     3961 ggctcgggcc ggatcggtgt ggacgtcgag tcgatgcggc cggacgtcga gatgcgctcg
     4021 ctggctcgcc ggttcttcac gcgctccgag aaccaggctc tcgaccggct gccggacgag
     4081 gagcttgcgc tcggcttcta cggatgctgg acctgcaagg aggcgttcgt gaaggcggtg
     4141 ggggagggct tgtcgttcga gctcgaccgg gtcgaggtcg cggtgcatcc cgcgccgccg
     4201 gggatcgtgt cggtcgacgg ggacgagggg gccgcggccg cgtggacgct tcaagcggtg
     4261 gaagtcggcc cgggatatgc cgcggcggtc gtcgtggact cgccgggggc cgaggtcgtg
     4321 gtgcgcgact ggccggcgag cgcggcggca tgaacgcctc cgccgagcac cggtcgccgg
     4381 agatagaggt cttccgtctg cgccgccgtc acctcaacga cgtgatcgcg atcgaagccg
     4441 acgtgttcgc acggccctgg agcacggcgt acttcatgca agagctgtcc aagccgaacc
     4501 gttcgtattt cgtggccgag tacgacggcg aggtgatcgg cttcgggggc atgatgttca
     4561 cgagcgagaa cgcggcccac gcgctgaacg tggcggtgac gccggcggtc caccggcggc
     4621 aggtagggac gaggctgctc tgcgtgctga tgatggacgc gatagcccgg g
//
LOCUS       DASQDO010000029         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_127298, whole genome shotgun sequence.
ACCESSION   DASQDO010000029 DASQDO010000000
VERSION     DASQDO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127298"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VEV43_00920"
     CDS             complement(<1..267)
                     /locus_tag="VEV43_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYP22114.1"
                     /translation="MKVAVTIEQCWHEIPGGTAVAALGMARGVAATGEAEVVGVSASH
                     RGPPPDPWVPPFEVRSLPLPRIALYESWHRLRAPAVERATGPVDV"
     gene            295..1254
                     /locus_tag="VEV43_00925"
     CDS             295..1254
                     /locus_tag="VEV43_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HYP22115.1"
                     /translation="MTSLAGKRVLVTGGNGFLGRHVQRKLQERGVGEVVVPRSGEHDL
                     TDRGQVAALWSKAEPDVVIHLAARVGGIGANMAAPADLYVSNLLMGTYVIEQARLAET
                     PKTVVLGTICAYPKYTPVPFLETSLWDGYPEETNAPYGVAKKALLVHAQANRAQYGQN
                     AIYLLPTNLYGPGDKFHPKVSHVIPALIKKCVDAVEAGDATIPVWGTGAATREFLYVD
                     DCAEGIVLATERYDGAEPVNLGSDHEVAIADLAELIAKLTGFEGVLVWDATKPDGQPR
                     RRVDATQARELFGFEAQTPLEEGLGQTIEWYRANRELAESATV"
     gene            1262..>2141
                     /locus_tag="VEV43_00930"
     CDS             1262..>2141
                     /locus_tag="VEV43_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYP22116.1"
                     /translation="MRVGYDAAPLLDAPTGVGRYAGELATALEARGVEIVRFAVSWGA
                     PRVDGVRRWRVPARAARASWRRWGRPPIERLTGRVDLVHATNFVLPALNEAPGVVTVH
                     DLSFFRDDTWPGGHRLRDLVPWSVERAAAVLVPTQAIADEVTARLGVSEGNVHVTPEG
                     VAPVFFGAGALSEAALGALGIPGPFVVATGTIEPRKNLPALLEAWRKVRGELDGWTLV
                     LAGPKGWGPELPETPGVLPIGWVGDETLPGLLAAAELFCYPSHYEGFGLPPLEAMAAG
                     TPAIAGRDTAAAEVLGD"
BASE COUNT          290 a          764 c          803 g          284 t
ORIGIN      
        1 gacgtcgacc gggccggtcg ctcgctcgac cgccggggcg cggagccggt gccacgactc
       61 gtacagggcg atccgcggga gcgggagcga acgcacctcg aacgggggga cccaggggtc
      121 ggggggcggc ccgcgatgtg acgccgacac gccgacgacc tcggcctcgc cggtggccgc
      181 cacgccgcgc gccatgccga gcgccgccac cgcggtgcct ccggggatct cgtgccagca
      241 ctgctcgatc gtcacggcaa ccttcaccgg gggattgtcc cctacgatgg ttcgatgacc
      301 tcgctcgccg gcaagcgcgt gctcgtcacc ggaggcaacg gcttcctcgg caggcacgtc
      361 cagcgcaagc tgcaggaacg cggcgtcggc gaggtggtcg tcccccgcag cggcgagcac
      421 gacctcaccg accgaggcca ggtcgcggcg ctgtggtcga aggccgagcc cgacgtcgtg
      481 atccacctcg cggcgcgcgt cggcggcatc ggggcgaaca tggccgcgcc ggccgacctc
      541 tacgtgtcga acctcttgat gggcacgtac gtgatcgagc aggcgcggct cgccgagacg
      601 ccgaagaccg tggtgctcgg gactatctgc gcgtacccga agtacacccc ggtgccgttc
      661 ctcgagacgt cgttgtggga cggctatccc gaggagacca acgcgcccta cggcgtggcg
      721 aagaaggcgc tgctcgtcca cgcgcaggcg aaccgcgcgc agtacgggca gaacgcgatc
      781 tacctgctgc cgacgaacct ctacgggccg ggcgacaagt tccatccgaa ggtgtcgcac
      841 gtgatccccg cgctgatcaa gaagtgcgtc gacgcggtcg aggccggcga cgcgacgatc
      901 ccggtgtggg ggacgggggc cgccacgcgc gagttcctgt acgtagacga ctgcgccgag
      961 ggcatcgtgc tcgccaccga acgctacgac ggggccgagc cggtgaacct gggctccgac
     1021 cacgaggtcg cgatcgccga cctcgccgag ctgatcgcga agctcaccgg cttcgagggc
     1081 gtgctcgtat gggacgcgac caagcccgac gggcagccgc gccggcgcgt cgacgccacc
     1141 caggcgcgcg agctgttcgg gttcgaggcg cagacgccgt tggaagaggg cctgggccag
     1201 acgatcgagt ggtaccgcgc gaaccgcgag ctcgcggagt ccgcgactgt ctgacgaggg
     1261 cttgcgcgtc ggctacgacg cggcccccct gctcgacgcg ccgaccgggg tcgggcgtta
     1321 cgcgggcgag ctggcgactg ctctcgaggc ccgcggcgtc gagatcgttc gcttcgccgt
     1381 ctcgtggggc gccccgcgcg tcgacggcgt ccgccgctgg cgggtcccgg cccgtgccgc
     1441 ccgcgcctcg tggcggcggt ggggccggcc cccgatagag cgcctcaccg gccgcgtcga
     1501 cctcgtgcac gcgacgaact tcgtcctccc ggctctgaac gaggcgccgg gcgtcgtgac
     1561 cgtgcacgac ctgtcgttct tccgcgacga cacctggccc ggcggtcacc ggctgcgcga
     1621 cctcgtgccg tggtcggtgg agcgagccgc ggccgtgctc gtgccgacgc aggcgatcgc
     1681 ggacgaggtc acggcccgcc tcggcgtctc cgaagggaac gtgcacgtca cgcccgaggg
     1741 cgtcgcgccg gtgttcttcg gtgccggcgc gctgtccgag gccgcgctcg gcgcgctcgg
     1801 gatccccggc cccttcgtcg tagcgaccgg cacgatcgag ccgcgcaaga acctgccggc
     1861 gctgctcgaa gcgtggcgga aggtgcgagg tgagctcgac ggatggacgc tcgtgctcgc
     1921 ggggccgaag ggctgggggc cggagctccc ggagactccc ggggtactgc cgatcgggtg
     1981 ggtcggcgac gagacgctgc ccggcctcct cgcggccgca gagctcttct gctacccgtc
     2041 ccactacgag ggcttcgggc ttccgccgct cgaggcgatg gcggcgggga cgccggcgat
     2101 cgccggccgg gacacggccg cggccgaggt gctgggcgat g
//
LOCUS       DASQDO010000030         8632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_433252, whole genome shotgun sequence.
ACCESSION   DASQDO010000030 DASQDO010000000
VERSION     DASQDO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433252"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VEV43_00935"
     CDS             complement(<1..555)
                     /locus_tag="VEV43_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22117.1"
                     /translation="MIRHAGAAIAVLLAALGAGMTPVAANDNGGDDAGARARAAEVTK
                     TVEARRVISSESGGDWCNVLVFYEFEDHPGTIEAALTYEYYGDQESASSSPPYDDVVD
                     TYTPTRVAPAGRHRILVNAVGISESADPSQCDEDWVPAREATFAPTGSVVLTIEQEDE
                     PKLTIELKTYAAEGGNADFNTVPPA"
     gene            complement(680..770)
                     /locus_tag="VEV43_00940"
     tRNA            complement(680..770)
                     /locus_tag="VEV43_00940"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(734..736),aa:Sec,seq:tca)
     gene            870..1220
                     /locus_tag="VEV43_00945"
     CDS             870..1220
                     /locus_tag="VEV43_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22118.1"
                     /translation="MGEAQLEGAPRLQAMDTDRNETGGTAPRRLGERAPAGAPESDDV
                     VRWVTAGIVAAAALLGLAVLVTIVVYALQPPGWLQTVLGLVMAAGAVVFAWLLASALR
                     KDDVEASRNASRRR"
     gene            1259..1810
                     /locus_tag="VEV43_00950"
     CDS             1259..1810
                     /locus_tag="VEV43_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22119.1"
                     /translation="MSTADLEIELARWRDPTAAPGSNALPLTVEQALAYRDAGNLPDE
                     HGRTLRLVLFARDLDEVRALGVKRLRFEPDYHSAPTWRREGSAPVNVVPLRGPDVAGD
                     ASPWWESPDVAALETEWRDTGRIAGLAVPAEYRGFVLKTVVALRAAGVAVDVDSVGAS
                     LARWLAPEHVREITDAMRAAAQG"
     gene            1871..2335
                     /locus_tag="VEV43_00955"
     CDS             1871..2335
                     /locus_tag="VEV43_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYP22120.1"
                     /translation="MSSIEQSIEVQVPVRTAYNQWTQFEDFPHFMEGVKEIKQLDDAS
                     LHWTVEIAGQVREFDAKITEQNPDERIAWTTIDGPHQAGVVTFHRLGDNQTKVMLQME
                     FEPEGIVEKAGAALGVVDSRVKGDLERFKSFIESRGQETGAWRGEVDQTKTS"
     gene            2408..3295
                     /locus_tag="VEV43_00960"
     CDS             2408..3295
                     /locus_tag="VEV43_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYP22121.1"
                     /translation="MDKRTRVAELSLVGIAAIWGLTFVMVQDAVERLPVNAFLAYRFL
                     PACALVTLVFRKELVKLSRAGWVAGLLMGVFLTSGYVFQTLGLQLTTAAKAGFITGLF
                     VVLTPVFGALFFGQRAGWAAWGAAVVSTGGLYLLSGGGSGGTASGDLLVLGCAASFAF
                     HILFTGRAVEDHHAGALLAVQLGVCGVVTCVAAAAGGDLELPRTAVEWNALVVTSLGA
                     TALGFFVQTYAQRHASPSRTALILASEPAFAGLFAYVLAGETLNATGWIGAGLIMAAI
                     VGVELLPHLRPPREPVPER"
     gene            3392..3913
                     /locus_tag="VEV43_00965"
     CDS             3392..3913
                     /locus_tag="VEV43_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22122.1"
                     /translation="MNASWRTRSVMAGAVAVLLALPVTSFAHHWAWKTDADDAESAMD
                     LKRVSVKVPKHKPGDGGQASCRLDFFGDPATEDINGRCEFDTKGGPKKDILILSYQNG
                     DKYEAAGSTWKGKYVTGGFPATWSRKDGDLILSIARKRLKGRSSHMNWKVSVEDGEDW
                     VPDKGWFRYDWKN"
     gene            4511..5992
                     /locus_tag="VEV43_00970"
     CDS             4511..5992
                     /locus_tag="VEV43_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYP22123.1"
                     /translation="MVRGDGTIGRYTISRPGRASYVSHQFRLALVDDEALLRTASYLR
                     LDGVETRERVFQEATATRRLVNSISSTGASNLDRIDELIAWPTSPTDEWTKGFLAGIF
                     DAEGGCSQGVFRICNCDSEIIEQTKRALDRFGFDYVMERQPGTNKPVDVIRLVGGLPE
                     RMRFFHTTDPAITRKRDIEGRALKSQARLRVVSIEDLGFEMPMYDITTGTGDFIANGV
                     VSHNCFARATHTYMDMDAGRDFETKIVVKVNAGQLLRKELRAKRWKGEGIAMGTATDP
                     YQRAEGRYKLMPPIIEALTDYRNPFSILTKGTLILRDLDLLVEAASVTDVSTAFSIGT
                     LDEDVWRRSEPGTPHPRKRIEAVARLNDAGIPCGVLMAPILPGISDRPDQLRDVMSAA
                     FDAGATHVSPILLHLRPKVREVYMDWLAENYPDLVERHETMYRKAYAAAADRKALAAT
                     VADIGQELGGPRPRKRKKRPQHNGAERSRAATAALRTEQLKLV"
     gene            complement(6014..6856)
                     /locus_tag="VEV43_00975"
     CDS             complement(6014..6856)
                     /locus_tag="VEV43_00975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYP22124.1"
                     /translation="MNKNVFKTYVLLAALGGFLVLIGRLFFGDSGPLIGLGLGLALVG
                     GSYWFSDKLAIRASRAQPVSEQEAPRLYGMVRELTTRGNLPMPRLYVIPSDQPNAFAT
                     GRNPKKAAVAVTEGIMKMMPEQELRGVIAHELAHVRNRDILIGSVAAAVATGITYIAY
                     IGMFFGGSDDDDGGGIGGLLLFFLAPMAAGLLQMALSRSREFEADRTAAAMIGTGEPL
                     ARALEKMEVAAKQLPMNVNPAQASAYIVNPLSGRKANMASLFSTHPPTEQRIERLRSW
                     RPGL"
     gene            6873..7889
                     /locus_tag="VEV43_00980"
     CDS             6873..7889
                     /locus_tag="VEV43_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HYP22125.1"
                     /translation="MLPEKWSLEARVMTMPLAERFTIARETWDVATAVFAIVRYGDAE
                     GYGECSPDAHWGETPETTVEQLESVDLGQLRGPFDLEGATRLLPAGSARCVLDVALHD
                     LAARLAGVPLRALLGVGDAPLPPTSVTVPISDPESMVARAKSFADHPIVKTKVGFDGD
                     VEAIEAIRSVFPGRIRVDANEGWSEDEAIDRLARLERFDVELCEQPIPMRNHEALRRV
                     TEASPIPVYADEDAGTSADVASLAGVVDGVNLKLRKAGGVRETWRAIATARAHAMGVM
                     LGCDLDSGIAMGAAAALAPLCDFIDLDGPLLLAEDPHPSVTYERGELKVPDAPGLGVT
                     KRPW"
     gene            7883..>8632
                     /locus_tag="VEV43_00985"
     CDS             7883..>8632
                     /locus_tag="VEV43_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HYP22126.1"
                     /translation="MVRSWLVLTDGYLETRNAKTAHGVLRYARDQVAAVLDRDPAGRS
                     LTEVMPEMRRDAPVVASLEEGRALGATSLLLGVATPGGWIPDHWRDWMLEAAGAGMEI
                     ANGLHRFLRDDDELVATARESGARLWDVRDPPDGIPLCSGKALEAPQRIVLTVGSDCA
                     VGKMTASLELAAAAEATGRKAEFVATGQTGILIAGTGIAVDRVIADFVSGAAEQLVVD
                     ADPAAEVLLVEGQGGLWHPAYSGVTLALLHGS"
BASE COUNT         1409 a         2912 c         2988 g         1323 t
ORIGIN      
        1 cgcgggcggg acggtgttga agtcggcgtt cccgccttct gctgcgtacg tcttcaactc
       61 gatcgtgagc ttgggctcgt cctcctgttc gatcgtgagc acgacgctgc cggtgggagc
      121 gaaggtcgcc tcacgcgccg gaacccaatc ttcgtcgcat tgggacggat cggcagactc
      181 ggagatgccc actgcattga cgaggatccg atggcgcccg gcaggtgcta cgcgggtagg
      241 ggtataggtg tcgacgacgt cgtcgtacgg ggggctcgaa gaggcggact cctggtcccc
      301 gtagtactcg tacgtgagtg ccgcttcgat tgtcccggga tggtcttcga actcgtagaa
      361 cacgagcacg ttgcaccagt caccgccgct ctcggaggag atgacgcgtc tcgcctcgac
      421 ggtcttcgtc acttcggcgg cgcgagcccg ggcgccggcg tcgtcgccgc cgttgtcatt
      481 cgcggcgacg ggtgtcatgc ctgccccgag ggccgcgagc agcacggcaa tggccgcccc
      541 tgcgtgtcga atcacgatgc ctcccgggtc gggtccgccg ccctagcttc gcaacctgac
      601 agctgcggcc gcctgtctga atagcgagaa aggactaaat acgggtactt gggcgggttc
      661 gccggcgcga acgaaagagc ggaggcggac gggaatcgaa cccgccggac gggtctcccc
      721 gccccaccgg ttttgaagac cgggagggac accagtcccc ggtcgcctcc tcggaacccc
      781 tccagtctca ccccgccggg cgagaccgcg gaccaaacga ggcaaacgag cgcaaccatc
      841 cgttgcttac caccgtctta agggtagaaa tgggtgaagc ccaactcgag ggagccccgc
      901 gcttgcaggc catggacacc gaccggaacg aaacaggcgg caccgcgccg cgccgtctcg
      961 gcgagcgggc gcccgccggc gcgcccgagt ccgacgacgt cgtccgctgg gtgaccgccg
     1021 ggatcgtggc ggccgcggcc ctcctcggcc tcgcggtcct ggtcacgatc gtggtctacg
     1081 cgctccagcc cccgggctgg ctccagacgg tcctcggcct cgtcatggcg gccggcgccg
     1141 tcgtcttcgc ctggctcctc gcctcggcgc tccgcaagga cgacgtcgag gcctctcgca
     1201 acgcgtcgag gcgccgatag ccgaccggca gcccgcgctc cgcgagggct ggtacctcat
     1261 gagcaccgcg gacctcgaga tcgagctggc tcgctggcgc gacccgaccg cggcccccgg
     1321 atccaacgct ctcccgctga ccgtcgagca agcgctcgcg taccgcgacg ccgggaacct
     1381 ccccgacgag cacggccgca ccctgaggct cgtcttgttc gctcgcgacc tcgacgaggt
     1441 ccgggccctc ggcgtcaagc gcctccgatt cgagcccgac taccactccg cgcctacgtg
     1501 gcggcgcgag ggatcggccc cggtgaacgt cgtgccgctg agggggccgg acgtcgccgg
     1561 cgacgcgtcg ccgtggtggg agtcgcccga cgtagcggcg ctcgagaccg agtggcggga
     1621 caccggccgc atcgcggggc tcgcagtgcc ggcggaatac cgaggcttcg tcctcaagac
     1681 cgtggttgcc ttgcgagcag cgggcgtcgc ggtcgacgtc gactccgtcg gcgcttcgct
     1741 ggcgcggtgg ctcgcgccgg agcacgtgcg cgagataacc gacgcgatgc gcgctgccgc
     1801 ccagggctga ggggtcagat ctggctcagt gggtatgtac gggttacatc cgactaacag
     1861 gaggtaacgc atgtcttcga tcgaacagag catcgaggta caggtccccg ttcgcaccgc
     1921 gtacaaccag tggacgcagt tcgaggactt cccccacttc atggagggcg tcaaagagat
     1981 caagcagctc gacgacgcca gcctgcactg gaccgtcgag attgccggcc aggtccgcga
     2041 gttcgacgcc aagatcaccg agcagaaccc cgacgagcgc atcgcgtgga cgacgatcga
     2101 cggcccgcac caggcgggcg tcgttacgtt ccaccgtctc ggcgacaacc agacgaaggt
     2161 catgctccag atggagttcg agcccgaggg catcgtcgag aaggcggggg ccgcgctcgg
     2221 cgtagtcgac agccgggtca agggcgacct cgagaggttc aagtcgttca tcgagagccg
     2281 cggccaggag accggcgcgt ggcgcggcga ggttgaccag accaagacct cgtaacgaag
     2341 cttccgcaag cggaaaaagg cgtcgggttc gccccggcgc cttttttcgt ggggttagcg
     2401 tgcggccgtg gacaagcgga cgcgcgtcgc cgagctctcg ctcgtaggca tcgccgcgat
     2461 ctggggcctg acgttcgtga tggtccagga cgcggtcgag cgccttcccg tcaacgcgtt
     2521 cctcgcatac cggttcctcc ctgcgtgcgc gctggtgacg ctcgtcttcc gcaaggagct
     2581 ggtgaagctc tcccgtgcgg ggtgggtcgc cggccttctc atgggcgtgt tcctgacgtc
     2641 gggatacgtc ttccagacgc tcgggctcca gttgacgacc gcggcgaagg ccgggttcat
     2701 cacgggcttg ttcgtagtcc tcacgccggt cttcggcgcc ttgttctttg gccagcgcgc
     2761 aggctgggcc gcatgggggg ccgcagtcgt gtccaccggc gggctgtatc tgctgtccgg
     2821 cgggggctcg ggtgggaccg caagcgggga cctgttggtg cttggctgcg cggcctcgtt
     2881 cgcgttccac atcctgttca cggggcgggc ggtcgaggat catcacgcgg gagccttgct
     2941 cgccgtgcag ctgggcgtct gcggtgtcgt gacgtgtgtc gccgcggccg ccggcgggga
     3001 cctcgagctt ccgcgcaccg cggtcgagtg gaacgcgctg gtagtgacgt cgctcggagc
     3061 gacggccctg ggcttcttcg tccagacgta cgcgcaacgc catgcatctc cgtcacgaac
     3121 ggcgctgatc ctcgcgagcg aaccggcgtt cgcggggttg ttcgcgtacg tgctggcggg
     3181 cgagaccctg aacgcgacgg ggtggatagg agcgggtctg atcatggcgg cgatagtggg
     3241 ggtcgagctg ctgccgcacc tcaggccccc gcgggagccg gtgcccgaac ggtaagccga
     3301 gaacgctcac gtctaagcat tacgacggat ggccccgtcc gcgccctcgc tgagacgatg
     3361 gcgcccatcc gtcgtccgct caggaggcta agtgaacgca tcgtggagaa cgcgctcggt
     3421 gatggccggc gcggtcgcgg tcctgctcgc acttccggtc acttcgttcg ctcatcactg
     3481 ggcgtggaag accgacgccg acgacgcgga gagcgcaatg gacctcaagc gcgtgtccgt
     3541 caaggtcccg aaacacaagc cgggcgacgg cgggcaggcg agctgcaggc tcgacttctt
     3601 cggcgatccg gcgacggagg acatcaatgg caggtgtgag ttcgacacga agggtggtcc
     3661 caagaaggac atcctgatcc tcagctacca gaacggtgac aagtacgaag cggcaggcag
     3721 cacgtggaag gggaagtacg tcaccggtgg cttcccggct acttggagcc ggaaggacgg
     3781 ggatctcatc ttgtcgatcg cccgcaagcg actcaagggt cgtagcagcc acatgaactg
     3841 gaaggtctcc gtcgaggacg gcgaagactg ggtgcctgac aagggatggt tccggtacga
     3901 ctggaagaac tgaccggggc ccggggccta gccgggatcg aacatacgtt ctataatgcc
     3961 cggatggcgg agaagctctt cgagctccag cgacggacct tcgatacgcc gcacttccgc
     4021 gggatcgagt tcatcgaggt cgaggccaag tcgaatcgtc aaccacgtcc cgggcaacta
     4081 cctcccgttc agctggacga tcaaccccta caggggctgc tcgcatgcgt gctcctactg
     4141 cagcccgggt tggacgccga tcctcagggc cgacggccgc acccggccgc tcgccgacct
     4201 gaaggtcggt gatcggatct acggcacgag ggtcgaggga cgctaccggc gttacgtgaa
     4261 gactgaggtc ctagcgcact ggcagacgat caagcgtgct cacaggatca tgctcgagga
     4321 tggaaccgcg atcgtcgcga gtggcgatca ccgcttcctg agcgaccgcg gctggaagca
     4381 cgtcacgggc gcggagcagg gcccgttgca gcggccgttc ctgacgacga acaacaagct
     4441 gatcggaacg ggtgccttcg cggagccacc gaaggtcgac gacgactatc ggcgcggata
     4501 cctgtgcgga atggtcaggg gtgacggcac catcggcagg tacacgatct cgcgacccgg
     4561 gcgggcgagc tacgtctcgc accaattccg gctggcgctg gtagacgacg aggccctgct
     4621 aagaacggcg agttaccttc ggctcgacgg agtcgagact cgagagcggg tctttcaaga
     4681 agcaacggcg acgcgcaggc tggtcaactc gatcagttcg acaggcgcgt cgaacctcga
     4741 ccggatcgac gagttgatcg cgtggccaac gtctcccact gacgagtgga ccaaagggtt
     4801 cttggcgggg atcttcgatg cagagggtgg ctgcagccag ggtgtcttca ggatctgcaa
     4861 ttgtgactcc gagatcatcg agcagacgaa gcgcgctttg gaccgcttcg ggttcgatta
     4921 cgtcatggaa cgacagcccg gcacgaacaa gcccgtggac gtcatccggc tcgtcggtgg
     4981 gctccccgaa cgcatgcggt tcttccacac cacggacccc gcgatcacac ggaagcgcga
     5041 catcgagggc agggcgctca aaagccaggc ccggcttcgc gtcgtctcta tcgaggatct
     5101 cgggttcgag atgcccatgt acgacatcac caccggcacc ggcgacttca tcgccaacgg
     5161 cgtagtcagc cacaactgtt ttgcccgcgc gacccacacc tacatggaca tggacgccgg
     5221 gcgggacttc gagaccaaga tcgtcgtcaa ggtcaatgcc ggccagctcc tgcgcaagga
     5281 gctccgcgcc aagcggtgga agggcgaggg catcgccatg ggcaccgcca ccgatcccta
     5341 ccagcgcgcc gagggccgct acaagctcat gcccccgatc atcgaggctc tcaccgacta
     5401 ccggaacccg ttctcgatcc tcacgaaggg cacgctgatc ctgcgcgacc tcgacctcct
     5461 cgtcgaggcg gcctccgtca ccgacgtctc gaccgcgttc tccatcggaa cgctcgacga
     5521 agacgtgtgg cgccgaagcg agcccggcac gccgcacccc cgcaaacgca tcgaagccgt
     5581 cgctcgcctg aacgacgccg gcatcccgtg cggcgtcctc atggcaccga tcctgccggg
     5641 catctcggac cggcccgacc agctccgcga cgtcatgtcc gcagcgttcg acgccggcgc
     5701 gacgcacgtc agcccgatcc tgctgcacct ccgcccgaag gtccgcgagg tgtacatgga
     5761 ctggctcgcc gagaactacc ccgacctcgt cgagcgccac gagacgatgt accggaaggc
     5821 ctacgcggcc gcggcggacc gcaaggccct cgcggcgacg gtcgcggaca tagggcaaga
     5881 gctgggaggc ccccgccccc gcaagcgcaa gaagaggcct cagcacaacg gcgccgaacg
     5941 ctcgcgcgcg gcgacggccg cgctacgaac ggaacagttg aagctcgtct aggccggggc
     6001 gcggctcgac gtgctagaga cccggcctcc agctgcgcag ccgctcgatc ctctgctccg
     6061 tcggcgggtg cgtcgagaac aacgacgcca tgttcgcctt ccggccgctc agcgggttca
     6121 cgatgtaggc ggacgcctgc gcgggattca cgttcatcgg cagctgcttc gccgcgacct
     6181 ccatcttctc gagggctcgg gccagcggct cccccgtccc gatcatcgcc gccgccgtac
     6241 ggtcggcctc gaactcgcgc gagcgggaca gagccatctg cagaagcccc gctgccatgg
     6301 gcgcgaggaa gaacagcagc aggccaccga tgcccccgcc gtcgtcgtcg tcgctcccgc
     6361 cgaagaacat gccgatgtag gcgatgtacg tgatcccggt cgcgaccgca gcggcgaccg
     6421 agccgatgag gatgtcgcgg ttgcggacgt gcgcgagctc gtgtgcgatg acgccgcgca
     6481 gctcctgctc cggcatcatc ttcatgatgc cctcggtgac cgctaccgcg gccttcttag
     6541 ggttccgtcc cgtcgcgaac gcattcggct ggtcgctcgg gatcacgtag aggcggggca
     6601 tcgggaggtt gccgcgcgtc gtcagctcgc ggaccatccc gtagagccgc ggcgcctcct
     6661 gctcggacac cggctgggcg cgcgacgcgc ggatcgccag cttgtcgctg aaccagtacg
     6721 acccgcccac gagcgcgagc ccgagcccaa ggccgatcag cggtccgctg tcgccgaaga
     6781 agagccggcc gatcagcacg aggaacccgc cgagggccgc cagcaggaca taggtcttga
     6841 agacgttttt gttcatgtag acattatggg ccgtgctccc ggaaaagtgg tcgctggagg
     6901 cccgcgtcat gaccatgccg ctcgccgagc ggttcacgat cgcgcgcgag acgtgggacg
     6961 tcgccacggc ggtcttcgca atcgtccggt acggcgacgc cgaggggtac ggcgagtgct
     7021 cccccgacgc ccactggggc gagacccccg agacgacggt cgagcagctc gaatcggtgg
     7081 atctggggca gctccgaggg ccgttcgacc tcgagggggc gacgcggctc ctcccggccg
     7141 gctcggcccg ctgcgtcctg gacgtcgcac tgcacgacct cgcggcgagg ctggccgggg
     7201 taccgctgag ggcgttgctc ggcgtcggcg acgcgcccct gcccccgacg tcggtgacgg
     7261 tcccgatctc cgaccccgag tcgatggtcg cccgggcgaa gtcgttcgcc gaccatccga
     7321 tcgtgaagac caaggtcggc ttcgacggtg acgtagaggc catcgaggcg atccgctcgg
     7381 tcttccccgg ccgcatccgc gtcgacgcga acgagggctg gagcgaagac gaggcgatcg
     7441 accggctcgc acgcctcgag cggttcgacg tcgagctgtg cgagcagccg atcccgatgc
     7501 gcaaccacga ggcgttgaga cgcgtcaccg aagcgtcgcc gatcccggtc tacgcggacg
     7561 aggacgcggg cacgtccgcg gacgtcgcgt ctctcgcggg tgtcgtcgac ggcgtcaacc
     7621 tcaagctgcg caaggcgggc ggcgtgaggg agacgtggcg cgcgatcgcg acggcgcggg
     7681 cgcacgcgat gggcgtgatg ctgggctgtg atctcgactc ggggatcgcg atgggcgcgg
     7741 cggccgcgct cgcgccgctg tgcgacttca tcgacctcga cgggccgctg ctgctcgcgg
     7801 aggacccgca cccgtcggtg acgtacgagc gcggcgagct gaaggttccg gacgcgcccg
     7861 gcctcggcgt gaccaaacgc ccgtggtgag gtcgtggctc gtcctcacgg acggctacct
     7921 cgagacccgc aacgccaaga ccgcacacgg agtcttgcgt tacgcgcgcg accaggtcgc
     7981 ggcggtgctc gaccgcgacc ccgcagggcg atccctgacc gaggtgatgc cggagatgcg
     8041 gcgcgacgcg ccggtggtcg cgtcgctgga agagggccgc gccctcggcg cgacgtcgct
     8101 gttgctcggc gtcgccacgc cgggcggctg gattcccgac cactggcgcg actggatgct
     8161 cgaggccgcg ggggccggca tggagatcgc gaacggcctc caccgcttcc tgcgcgacga
     8221 cgacgagctc gtcgccaccg cgcgcgagag cggcgcgcgc ctgtgggacg tgcgcgaccc
     8281 tcccgacggc ataccgctgt gctcgggcaa ggccctagag gcgccgcagc ggatcgtcct
     8341 gacggtcggc agcgactgtg cggtcgggaa gatgaccgcg tccctcgagc ttgccgcggc
     8401 cgccgaggcg accgggcgaa aggccgagtt cgtcgcgacc ggccagaccg ggatcctgat
     8461 cgcggggacg gggatcgccg tcgaccgcgt catcgccgac ttcgtctcgg gcgccgcgga
     8521 gcagctcgtg gtcgacgcgg accccgctgc ggaagtgctg ctcgtcgagg gacagggcgg
     8581 gctgtggcac cccgcgtact ccggcgtgac gctggcgctg ctgcacggct cg
//
LOCUS       DASQDO010000031         4079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_494614, whole genome shotgun sequence.
ACCESSION   DASQDO010000031 DASQDO010000000
VERSION     DASQDO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494614"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VEV43_00990"
     CDS             <1..1096
                     /locus_tag="VEV43_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22127.1"
                     /translation="APECVPRLAGRPLYEISHDGRLAALPRAAFAALGIRERDDIQRL
                     LRDDVGALGDDLLVIAEEFGEWEDARRRIDLLAVDRSGRLVVIELKRTESGGHMDLQA
                     VRYAAMVSAMDFEDVVAAYSRHLAARAPGDARDARAELEDFLDAGDPDEGPAISTDVR
                     IILVGADFGREITTTVLWLNRFDGMDVRCVRMIPYDVGDNRVLVDVQQVIPLAEAADY
                     QVRIRRKETARERATGSTRDYTRFHVVVDGHELPAENKRNAVRLMIEQLTARGAPLDL
                     VYETISKRMKVLAGVYRTGDGVREALVAEYGSIDVDRWFTESPFVDEAASQTYVLSNQ
                     WGKKTEQRLQDLADAFPDSKVTFRRAEPGD"
     gene            complement(1108..1182)
                     /locus_tag="VEV43_00995"
     tRNA            complement(1108..1182)
                     /locus_tag="VEV43_00995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1145..1147),aa:Ile,seq:gat)
     gene            complement(1285..2664)
                     /locus_tag="VEV43_01000"
     CDS             complement(1285..2664)
                     /locus_tag="VEV43_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22128.1"
                     /translation="MNHLRQLVVAALAAALLAALVAQPAGATHNADDHSDNVRMLAEH
                     AIIITPDDPETPDANEEVRALGSDLAFQKDLLVAGSYEGVGFFKLHKKAPYLEQIGYF
                     ACPGSQGDVSVWGDLVFVSVDAPRDKPECDSVGANQAATAQGADWEGVRIIDISNMKR
                     IKQVAAIRTDCGSHTHTLVPGGKGKLYVYVESYPLTGQSGSCNAVGHRKISVIEVPLS
                     KPAKAEVVTTPDLLSTIGCHDITVDVKRKLAVGACVSESQVWDVSKPTEPKLLARIHN
                     PKIQIHHSTGFTWDGKYLVLGDEFGGAAAGGCVGNSQVPMGAMWFYDISDPTAPKEAG
                     YWAPPRVFTPPDDADEAQRPRCTTHNFNVLPMKDAKRYIAVSSYYSVALSVVDFSDPT
                     APEELGYYLPKESGTNPDSWSAYWYNGLIYSNDHGSAAGIRVYEMDGLSTKEVKTFKT
                     RLNPQTQMM"
     gene            complement(2788..>4079)
                     /locus_tag="VEV43_01005"
     CDS             complement(2788..>4079)
                     /locus_tag="VEV43_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22129.1"
                     /translation="LGSIPASAGHDDSLHSDNVKLVTRKPIKIDKDIFAQGSDLAFQG
                     KLIVAGTYEGTAFFKILKKGRIKQVGFHHCPGSQGDVSVWGKYVFVSIDTASTNSGGA
                     AGCNNTDESTGKEGLRIIDISDLKAPRQVKFVETPCGSHTHTLSPAGNTLYAYIESYP
                     LGAPTDTCSPASHRKVSIVKIPLSDPTKAEISGELDVSPSIGCHDLTTFPAKKLAIAA
                     CISESQVWDISDPAQPVKLAVINNPSIQVHHSASFTWDGKYAILSDEYGGASGGGGCT
                     GEQDSKVGAMYFYDITDPSAPTLVGDYSLPRVPPADYQDENRTFRCTTHLYNILPMKD
                     PAKYVAASSYYEGGISVVDFSDPAAEEEVGYYLDLPAGQLPDTWSSYWYNGYIFTNDH
                     ESSHGLRVFKMKGLGKKQVHYFKGRFNPQVQVASFRR"
BASE COUNT          647 a         1330 c         1379 g          723 t
ORIGIN      
        1 cgcgcctgaa tgcgtgccta gactcgccgg gaggcctctc tacgagatct cgcacgacgg
       61 gcggctcgcg gccctccccc gcgccgcttt cgccgctctc gggatacgcg agcgcgacga
      121 catccagcgt ctcttgcggg acgacgtcgg cgccctgggc gacgacctcc tcgtgatcgc
      181 ggaggagttc ggcgaatggg aggacgcgcg ccggcggatc gacctcctgg cggtcgacag
      241 gagcggacgg ctcgtcgtga tcgagctgaa gcgcaccgaa agcggcggtc acatggacct
      301 ccaggccgtc cgctacgcgg cgatggtctc ggccatggac ttcgaagacg tggtcgcggc
      361 gtactcgcgc cacctcgccg cccgcgcccc cggggacgca cgcgacgcgc gggcggagct
      421 cgaggacttc ctcgacgcgg gcgaccccga cgaaggtccc gcgatctcga ccgacgtccg
      481 catcatcctg gtcggggcgg acttcgggcg agagatcacg accaccgtcc tatggctgaa
      541 ccgcttcgac gggatggacg tcaggtgcgt ccgcatgatc ccgtacgacg tcggcgacaa
      601 ccgtgtgctg gtcgacgtcc agcaggtcat cccgttggcg gaagccgcgg actatcaggt
      661 ccggatcagg cggaaggaga cggcacgcga acgcgctacc ggcagcactc gcgactacac
      721 gcggttccat gtggtcgtgg acggccacga gctgccggcc gagaacaagc gcaacgccgt
      781 tcgtctgatg atcgagcagc tcaccgctcg gggcgcgccc ctggacctcg tctacgagac
      841 catctcgaaa cgaatgaagg tcttggcggg ggtctaccgc acgggggacg gggtgcggga
      901 ggcactcgtc gcggagtacg ggtcgatcga cgtcgacagg tggttcacgg agtcaccgtt
      961 cgtggacgag gcggcgagcc agacctacgt cttgtccaat cagtggggca agaagaccga
     1021 acagaggctc caggacctcg ccgatgcctt cccggactcg aaggtgacgt tccgccgcgc
     1081 cgaacccggc gactgatcgc ggggcagtgg gcccagatgg atttgaacca tcggcctcac
     1141 ccttatcagg ggtgcgctct aaccgagctg agctatgggc cctcgttgcc gccgcatcct
     1201 acaaaggaag aaggggcgcc gaagcgcccc ttcccctccc cccgtggggc cctccgccag
     1261 tagggccacc atccgtaggt ccgcctacat catctgcgtc tgcgggttca gacgcgtctt
     1321 gaaagtcttc acttccttcg tcgagaggcc atccatctcg tagacgcgga tcccggccgc
     1381 cgagccgtgg tcgttcgaat agatcaagcc gttgtaccag tacgccgacc acgagtccgg
     1441 gttcgtcccg ctctccttcg gcaggtagta gccgagctcc tcgggcgccg tcgggtcgct
     1501 gaagtcgacg accgagaggg cgaccgagta gtacgacgac accgcgatgt acctcttagc
     1561 gtccttcatc gggagcacgt tgaagttgtg cgtcgtgcag cgcgggcgct gggcctcgtc
     1621 ggcgtcgtcc ggcggcgtga acacccgtgg cggcgcccag tagccggcct ccttcggcgc
     1681 cgtcgggtcg gagatgtcgt agaaccacat cgcgcccatc gggacctgcg agttcccgac
     1741 gcaaccgccg gcggccgcgc ccccgaactc gtcaccgagc acgaggtact tgccgtccca
     1801 cgtgaacccc gtcgagtggt ggatctggat cttcgggttg tggatgcgtg cgagcagctt
     1861 cggctccgtc ggcttcgaca cgtcccacac ctgtgactcg ctgacgcagg cgccgaccgc
     1921 gagcttgcgc ttcacgtcga cagtgatgtc gtggcagccg atcgtcgaga gcaggtccgg
     1981 cgtcgtgacg acctcggcct tggccggctt cgacagcggc acctcgatca ccgagatctt
     2041 gcggtggccg actgcgttgc acgagccgct ctggccggtc agcgggtacg actcgacgta
     2101 gacgtagagc ttgcccttgc cgccggggac gagcgtgtgc gtgtgcgacc cgcagtctgt
     2161 ccggatcgcc gcgacctgct tgatgcgctt catgttcgag atgtcgatga tgcggacgcc
     2221 ctcccagtcg gcgccctgcg cggtcgcggc ctggtttgcg ccgacggagt cgcactcggg
     2281 cttgtcgcgc ggggcgtcga ccgacacgaa gacgaggtcg ccccaaaccg acacgtcacc
     2341 ctgcgagcct gggcacgcga agtagccgat ctgctcgagg tacggggcct tcttgtggag
     2401 cttgaagaac ccgacgccct cgtaggagcc cgcgacgagc aggtccttct ggaacgcgag
     2461 gtcggagccc aacgcgcgca cctcttcgtt cgcgtcgggc gtctccgggt cgtcgggcgt
     2521 gatgatgatg gcgtgctcgg cgagcatcct cacgttgtcc gagtggtcgt cggcgttgtg
     2581 cgtggcaccc gccggctgtg cgaccagcgc cgccaagagc gccgctgcca aggcggcgac
     2641 taccagctgc ctcaggtggt tcatctcttc tccccatgcg tccgttggtc aggttctaag
     2701 gctttcgctg gagttggcag gattccttcc cgaacaaaaa ggcccccggg gttcccgggg
     2761 gccttcgtgc tcggagagcc cgacgggcta cctccggaag gaagcgacct ggacctgcgg
     2821 gttgaaccgg cccttgaagt agtggacctg cttcttcccg aggcccttca tcttgaagac
     2881 gcgcagcccg tgggacgact cgtggtcgtt cgtgaagatg tagccgttgt accaatagga
     2941 cgaccaggtg tccgggagct ggccggcggg caggtccagg tagtacccga cctcctcttc
     3001 ggccgcgggg tccgagaagt cgacgaccga gatcccgccc tcgtagtagg aggaggcagc
     3061 gacgtacttc gccgggtcct tcatcgggag gatgttgtag aggtgcgtgg tgcagcggaa
     3121 cgtcctgttc tcgtcctggt aatccgccgg cgggaccctc ggcagtgaat agtcgccgac
     3181 cagcgtgggg gccgaagggt cggtgatgtc gtagaagtac atcgccccca ctttgctgtc
     3241 ctgctcgccc gtgcacccgc caccaccgga cgcgccgccg tactcgtcgc tgaggatggc
     3301 gtacttcccg tcccacgtga acgacgccga gtggtgcacc tggatcgacg ggttgttgat
     3361 gaccgcgagc ttgacgggtt gggcggggtc gctgatgtcc cacacctgcg actccgagat
     3421 gcacgccgcg atggcgagct tcttcgcggg gaacgtagtc aggtcgtgac agccgatcga
     3481 aggcgacacg tcgagctcgc ccgagatctc cgccttcgtc gggtcgctga gcgggatttt
     3541 cacgatcgag accttgcggt ggctcgccgg gctacacgtg tcggtcggag cgccgagcgg
     3601 atacgactcg atgtacgcgt agagggtgtt ccccgccggc gacagcgtgt gcgtatgcga
     3661 cccgcagggc gtctcgacga acttgacctg ccgcggcgcc ttcaggtccg agatgtcgat
     3721 gatgcgaagg ccttccttgc cggtcgactc gtcggtgttg ttgcaacccg cggccccacc
     3781 gctgttcgtc gaggccgtgt cgatcgagac gaagacgtac ttgccccaaa ccgacacgtc
     3841 gccctgggag ccggggcagt ggtggaagcc gacctgcttg atgcgcccct tcttgaggat
     3901 cttgaagaac gccgtcccct cgtaggtgcc ggcgacgatc aacttgccct ggaaggcgag
     3961 gtccgacccc tgggcgaaga tgtccttgtc gatcttgatc ggcttgcggg tgacgagctt
     4021 cacgttgtcc gaatgcagtg agtcgtcgtg cccggcgctg gcagggatcg agcccagca
//
LOCUS       DASQDO010000032        10237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1045340, whole genome shotgun sequence.
ACCESSION   DASQDO010000032 DASQDO010000000
VERSION     DASQDO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045340"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VEV43_01010"
     CDS             complement(<1..341)
                     /locus_tag="VEV43_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYP22130.1"
                     /translation="MASIDYLSGLNPVQREAVTHAGGPVLVVAGAGSGKTRVLTHRIA
                     YLVHTGMARHGEILAITFTNKAAQEMRHRVEGLVGRRTRAMWVMTFHAACARILRAEA
                     ERLGYTRQFTI"
     gene            complement(350..1870)
                     /locus_tag="VEV43_01015"
     CDS             complement(350..1870)
                     /locus_tag="VEV43_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HYP22131.1"
                     /translation="MGVAETPGIGRDYRNILSGTSQNVVGIAVAAVATLASNLLISNT
                     LGREAFGVVTVVTQAAFVLSFATRAGMDMAVLRDVAVEVGVGRFDRIRVPVARAALIA
                     CVVSSIVAVVAVAFAGAVRALFSIEGDLGRWVVQAGAAGLPFLALANVWLSATRGLKI
                     MRYTLYVFWAGQPVLWVVLMLAGWRISETAWMAVAAYSASWLLATFAAAYFWRRESKG
                     WTAAPMEAGALSRLARYAGPRAPAALFSQLLFWTDLFVLTRYASGDDIGIYSAALRAG
                     QVLVLFLTSVSLMFSPFVADLHNRGETERLDKLFKALTRWTIAATIPLFLLLLIAPDA
                     ALRIFGSGFTGGQAALLIVLAGQFVNIATGSVGFVLIMVGRTGWDLVVYAGSLALNLA
                     LAFWLCPRYGMEGAAVANAVTFALSKWARLALVHRFVKIQPYDRRYGRLVLPSLVGAA
                     VMWAVHAAVGGGGLLDLVATGLVGTAAYVAVYLVGGLTPEERAGVAKIIARVRAGG"
     gene            complement(1861..3420)
                     /gene="guaA"
                     /locus_tag="VEV43_01020"
     CDS             complement(1861..3420)
                     /gene="guaA"
                     /locus_tag="VEV43_01020"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYP22132.1"
                     /translation="MPHSSSEPEHPPVLVLDYGAQYAQLIARRVRECHVYSEIVPHDA
                     PVDEIAARNPAGIILSGGPKSVHQPGAPSVDPRLFDLEVPVLGICYGQQAMAQALGGD
                     VARTGVAEFGKTDLDVKAPQSVLFQELPVEQTVWMSHNDAVVTPPEGFRPTAATPASP
                     VAAFEDPARGLYGVQFHPEVAHTPWGTALLKNFLYDACDLLPGWTMTSIIEAGIEAVR
                     AQVGDERIVCGLSGGVDSSVAAALVHRAVGDQLTCVFVDHGLLRQGEAEQVEDTFRRH
                     LKIDLIHVKAADRFLEKLAGVTDPEAKRKIIGETFIRVFEEVARDDLGDARFLVQGTL
                     YPDVIESGTKDAAKIKSHHNVGGLPDDMKFELVEPLRNLFKDEVRRVGEELGLPEEIV
                     WRQPFPGPGLAVRIVGDVTPERLELLRAADAIGLEEIKRAGLTREIWQSFAVLPCVRS
                     VGVMGDERTYGYPIVLRAVTSEDAMTADWARLPYDLLERISTRVVGEVPGINRVVYDV
                     TSKPPGTIEWE"
     gene            complement(3429..4337)
                     /locus_tag="VEV43_01025"
     CDS             complement(3429..4337)
                     /locus_tag="VEV43_01025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HYP22133.1"
                     /translation="MSEPKNVEQLLDLGTRVLSDSSHIFEDHDNAAEARELLAFSLDV
                     ATDDLDDLDEHDGEVPRRIRDRYLSLVARRAGGEPFPFLTGRIEFWGMELKVRPGAFV
                     PRPSSELTVQRALRKLRRRKGPVVVDVCTGAGPIALALADELPTAEVWGADISVEGLA
                     QGRENAKLHGLDNVRFKKSDMYDGLPARLRGNVDLITGHVPYVPAGEVDDLPAEVREF
                     EPIYTLTDESADGLGLMRRAVGESVEWLKPGGWLLLELSHDLPPKIRRLVRKAGLEDH
                     GVAKDDDDLSVVVEARKTPEPRRAAR"
     gene            complement(4360..4923)
                     /locus_tag="VEV43_01030"
     CDS             complement(4360..4923)
                     /locus_tag="VEV43_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYP22134.1"
                     /translation="MGTPPSRPAPAGSVDKARAVAIAAALDPIDRKILRFLREDGRMS
                     HAAIAKEVGLSGPAVHERVRKLEQRGVIAGYTVMLDPEALGKPHVAFALVTLSEGNEF
                     AADDPIVARICDEPDVLEFHRIAGQDCYLIKIRSATNKDLEKLLRRIRSIRGVARSRT
                     TIVLSTELERPTIAVPEPEAEDVEPAR"
     gene            complement(5477..6553)
                     /locus_tag="VEV43_01035"
                     /pseudo
     CDS             complement(5477..6553)
                     /locus_tag="VEV43_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
     gene            7036..7605
                     /locus_tag="VEV43_01040"
     CDS             7036..7605
                     /locus_tag="VEV43_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HYP22135.1"
                     /translation="MKRLAPWLWVASVLMMVMGVPGANVGADALQRLSGFDRSELRGI
                     ETSESAAGLMKFRGGTFQSRPAATPEPTPPPTPTPEVVVPAAPVAPVAPAVEVAPPAP
                     EGSVTAIIYAAAAEYGLSGPYLLSVAACESGLNPSAVNSAGYYGLFQFSSSTWASNGY
                     GSIYDPVAQARTAARMIAGGGAGHWPSCA"
     gene            complement(7602..8837)
                     /locus_tag="VEV43_01045"
     CDS             complement(7602..8837)
                     /locus_tag="VEV43_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP22136.1"
                     /translation="MSDSPRTLRQNVRELPRAAWIVCAGAFVNRFGSFVSVFLVLYLT
                     AEGYSAAAAGTAAGAYGVGHLAASAIGGYLTDRIGRRETIVLSMYSAAAAMLALSQAQ
                     GLAAITFFVFLIGLSSELYRPASSALLADLVPPEQRVAAFAVWRLAVNAGFALGPAAA
                     GFLADRSFFWLFVGDALTSVVFGTVALVSLPRGRPPAPGPHATGGSIRSMLRHRAFVA
                     FLVASVGITYVYLQSMSVLALHVKAEGLSTSDYGLLVSLNGLLVVFLELPVASVTQRL
                     DARRVIAVGFFVTGLGFALTAFAGTMPLLAATVVLWTIGEVIDAPVSSAYVAELAPEH
                     MRGRYMGAWGLTWSLGLMLAPSVGARFFAAAPDGAWLACGVIGAVAAAIVLLGGRPKM
                     VEPRLPLGAPGPELPRMEG"
     gene            8903..9334
                     /locus_tag="VEV43_01050"
     CDS             8903..9334
                     /locus_tag="VEV43_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22137.1"
                     /translation="MSATDPRTRWGLVLHVVVALSLWSWFDVPVDPAGESGLAATLLV
                     LITASVPFALLFAALGARFAERTSVTRVVAWVGALVYGIWLGGWASVVGTSGGELECD
                     PPDCRSSWGPRVLTFVTATIIVLVCARIEAALRKRWSSEPS"
     gene            complement(9318..9740)
                     /locus_tag="VEV43_01055"
     CDS             complement(9318..9740)
                     /locus_tag="VEV43_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22138.1"
                     /translation="MFITRATRHDRADIAELYEEYGWGDENLDKGKAFIARDGGVVGA
                     LRLVEVEPNTLVVDGVLVKEGRREKGIGRQLMQTAMNSRGGTMYLCCHDNRMRFYSHF
                     GFEEISIDDAPEPVRKYWEEVGDWPTEPGHEHFFMKAR"
     gene            complement(9743..>10237)
                     /locus_tag="VEV43_01060"
     CDS             complement(9743..>10237)
                     /locus_tag="VEV43_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HYP22139.1"
                     /translation="EAGGPGAQIGRPHFAAVLVRHGAVPDYQTAFDEYLAKGAKAYLT
                     RKLYSPVEATELMVRSGVVPVLAHPFTLKLPIEQLETFVDELVSVGLRGIEGYHGDLP
                     EIEQEPYRALGRAKGLIVSGGSDYHGDMRPDRGLPGGKHGVEVPDAVLEELRSAAREL
                     QGVA"
BASE COUNT         1547 a         3528 c         3653 g         1509 t
ORIGIN      
        1 tagatcgtga actggcgcgt gtagcccagc cgctcggcct cggcgcgcag gatccgcgcg
       61 caggcggcgt ggaaggtcat gacccacatg gcccgggtcc gccggccgac gagcccctcg
      121 acgcggtggc gcatctcctg cgcggccttg ttcgtgaacg tgatcgcgag gatctcgccg
      181 tgccgcgcca tgccggtgtg cacgaggtac gcgatccggt gcgtgagcac gcgggtcttg
      241 ccggacccgg cccccgcgac caccaggacc gggccccccg cgtgcgtgac cgcttccctc
      301 tggaccgggt tcagccccga gaggtagtcg atggatgcca tgcctgtgat tatccgcccg
      361 cccggacccg ggcgatgatc ttcgcgaccc cggccctctc ctccggcgta agcccgccga
      421 cgaggtagac ggccacgtag gcggccgtcc ccacgaggcc ggtcgcgacg aggtcgagga
      481 gcccccctcc ccctaccgct gcgtggacgg cccacatgac cgcggcgccc acgagcgacg
      541 gaaggacgag ccgtccgtag cggcggtcgt agggctggat cttcacgaac cggtgcacga
      601 gcgcgagccg cgcccacttc gacagcgcga acgtcacggc gttggcgacc gccgccccct
      661 ccatgccgta gcgggggcag agccagaacg cgagcgcgag gttcagcgcc agcgagcccg
      721 cgtagaccac gaggtcccac ccggtccggc cgaccatgat gaggacgaag ccgacgctcc
      781 ccgtggcgat gttcacgaac tggccggcga gcacgatcag cagcgcggcc tggcctccgg
      841 tgaaccccga cccgaagatg cgcagcgcgg cgtcgggcgc gatcagcaac aacaggaaga
      901 gcgggatcgt cgcggcgatc gtccagcgcg tcagcgcctt gaagagcttg tcgaggcgtt
      961 cggtttcgcc gcggttgtgg aggtcggcga cgaacgggct gaacatcagg ctcaccgacg
     1021 tgaggaacag cacgaggacc tgacccgcgc gcagggcggc cgagtagatc ccgatgtcgt
     1081 cgccgctcgc gtagcgggtg agcacgaaca ggtctgtcca gaacagcagc tgtgagaaca
     1141 gcgcggcggg ggcgcggggg cccgcatagc gcgcgagcct cgagagcgca ccggcctcca
     1201 tcggcgcggc ggtccagccc ttgctctcgc ggcgccagaa gtacgcggcg gcgaacgtcg
     1261 cgaggagcca cgacgccgag tacgcggcga ccgccatcca cgcggtctcc gagatccgcc
     1321 agcccgcgag catcagcacg acccacagca cgggctggcc ggcccagaag acgtagagcg
     1381 tgtagcgcat gatcttgagc ccgcgcgtcg cggagagcca cacgttcgcg agcgcgagga
     1441 acgggagccc ggcggcgccg gcctggacga cccaccgtcc gaggtctccc tcgatggaga
     1501 acagcgcacg tacggccccg gcgaacgcga ccgcgacgac ggcgacgatc gagctgacga
     1561 cgcacgcgat gagcgcggcc cgcgccaccg gcacgcggat gcggtcgaac cgcccgacgc
     1621 cgacctccac ggcgacgtcg cgcagcacgg ccatgtccat cccggcgcgc gtggcgaacg
     1681 agagcacgaa agccgcttgg gtgacgacgg tgacgacgcc gaaggcctcg cgcccgagcg
     1741 tgttggagat caggaggttc gatgcgagcg tggcgaccgc ggcaaccgcg atgccgacta
     1801 cgttctgtga cgtcccagag aggatgtttc ggtaatcacg accaatgccg ggtgtctctg
     1861 ctactcccat tcgatggtgc cgggcggctt cgacgtgacg tcgtagacga cccggttgat
     1921 ccccggcacc tcgccgacga cgcgggtcga tatccgctcg agcaggtcgt acgggagccg
     1981 cgcccagtcg gccgtcatcg cgtcctcgct cgtcaccgcg cgcagcacga tcgggtagcc
     2041 gtaggtccgc tcgtcgccca tgacgccgac ggagcggacg cacgggagga ccgcgaacga
     2101 ctgccagatc tcacgcgtga gcccggccct cttgatctcc tccagcccga tcgcgtcggc
     2161 cgcccgcaac agctcgagcc gctccggcgt gacgtcgccg acgatccgga ccgcgaggcc
     2221 cgggccgggg aacggctggc gccacacgat ctcctcgggc aggcccagct cctcgccgac
     2281 gcgcctgacc tcatccttga agaggttgcg cagcggctcg acgagctcga acttcatgtc
     2341 gtcggggagg ccgccgacgt tgtggtggct cttgatcttc gccgcgtctt tcgtcccgga
     2401 ctcgatgacg tcggggtaga gcgtgccctg cacgaggaag cgagcgtcgc cgaggtcgtc
     2461 gcgcgccacc tcctcgaaga cgcggatgaa cgtctcgccg atgatcttgc gcttggcctc
     2521 ggggtccgtg acgccggcga gcttctcgag gaagcggtcc gcggccttca cgtggatgag
     2581 gtcgatcttc aggtggcggc ggaacgtgtc ctctacctgc tcggcctctc cctgacggag
     2641 gagcccgtgg tcgacgaaca cgcatgtgag ctggtcgccc acggcgcggt gcacgagggc
     2701 cgcggcgacg ctcgagtcga cgccaccgga gaggccgcag acgatgcgct cgtcaccgac
     2761 ctgcgcgcgg accgcttcga tccccgcctc gatgatcgac gtcatcgtcc agccgggcag
     2821 caggtcgcac gcgtcgtaga ggaagttctt cagcagcgcg gtcccccacg gcgtgtgagc
     2881 gacctcgggg tggaactgga cgccgtagag cccgcgggcc gggtcctcga acgcggcgac
     2941 gggagaggcg ggggtcgctg ccgtcgggcg gaacccctcg ggcggcgtaa cgacggcgtc
     3001 gttgtggctc atccacacgg tctgctcgac cgggagctcc tggaacagca cggactgtgg
     3061 agccttgacg tcgaggtcgg tcttgccgaa ctccgctact ccggtgcgag cgacgtcacc
     3121 gccgagcgcc tgcgccatcg cctgctgtcc gtagcagatg ccgagcacgg gcacctcgag
     3181 gtcgaacaga cgcgggtcga ccgacggcgc gccgggctgg tgcacggact tcggcccccc
     3241 cgacaggatg atcccggccg ggttgcgcgc ggcgatctcg tcgaccggcg cgtcgtgcgg
     3301 gacgatctcg gagtaaacgt ggcactcgcg cacgcggcgg gcgatcagct gcgcgtactg
     3361 cgcgccgtag tcgaggacca gtacgggggg gtgctcgggc tcagaagaag agtgcggcat
     3421 gagccactct aacgcgcggc cctgcggggc tccggggttt ttcgcgcctc gaccacgacg
     3481 ctgaggtcgt cgtcgtcctt cgcgaccccg tggtcttcga gccccgcctt gcgcacgagc
     3541 cggcgaatct tcggcggaag gtcgtgcgac agctcgagca ggagccagcc cccgggcttg
     3601 agccactcga cggactcccc cacggctcgg cgcatgaggc ccagcccgtc cgcggattcg
     3661 tcggtcagcg tgtagatcgg ctcgaactcg cgcacctcgg cggggaggtc gtcgacctcg
     3721 ccggccggga cataggggac gtggccggtg atcaggtcga cgttgccgcg caagcgtgcc
     3781 ggcagcccgt cgtacatgtc gctcttcttg aagcggacgt tgtcgaggcc gtggagcttc
     3841 gcgttctcgc ggccctgcgc tagcccttcg acggagatgt ccgcgcccca cacctccgcg
     3901 gtcgggagct cgtcggcgag cgcgagcgcg atcggccccg cacccgtgca cacgtcgacg
     3961 acgaccgggc ccttgcggcg gcgcagcttg cgcagcgcgc gctggacggt cagctccgac
     4021 gagggccgcg gcacgaacgc gccgggcctg accttgagct ccatgcccca gaactcgatg
     4081 cggccggtga ggaacgggaa cggctcgccg ccggcacgcc gcgcgacgag cgagaggtag
     4141 cgatcacgga tgcgccgcgg gacctcgccg tcgtgctcgt cgaggtcgtc gaggtcgtcg
     4201 gtcgcgacgt cgagcgagaa agcgaggagc tcgcgcgcct cggccgcgtt gtcgtggtct
     4261 tcgaagatgt gggagctgtc gctgaggacg cgcgtgccga ggtccagcag ctgttcgacg
     4321 ttcttgggct cgctcatggg agcccaagag tacgggtccc tagcgcgcgg gctctacgtc
     4381 ttcggcttcg ggctcgggca ccgcgatcgt cggacgctcg agctcggtcg acaggacgat
     4441 cgtcgtacgc gagcgggcga cgccgcggat cgagcggatg cggcgcagca gcttctcgag
     4501 gtccttgttg gtcgcggacc ggatcttgat caggtagcag tcctggccgg cgatgcgatg
     4561 gaactcgagc acgtcgggct cgtcgcagat gcgcgcgacg atcgggtcgt ccgccgcgaa
     4621 ctcgttgccc tcggagagcg tgacgagcgc gaacgcgacg tgcggcttgc ccagcgcctc
     4681 ggggtcgagc atgacggtgt agccggcgat gacgccgcgc tgctcgagct tgcgcacgcg
     4741 ctcgtggacc gccgggcccg agagcccgac ctccttcgcg atcgccgcat gcgacatgcg
     4801 gccgtcctcg cggaggaacc gcaggatctt gcggtcgata gggtcgaggg cggcagcgat
     4861 cgctaccgcc ctcgccttat cgaccgagcc ggccggagcc gggcgggacg ggggggtgcc
     4921 cacccggggg tcaggcccgg atcgaggtgg ttcaggatgt gtccgacgtt catgtgagta
     4981 cctcttcgtt cggtgtttcc gtcccggccg gattggtccg gccgggaatc ctcgacgggc
     5041 ccgtgttacg ccctgtcggt gcggtgcagg gcccaatcgt ccccgtcacg gctgtctcgc
     5101 ccgtagcggg acgcaagacc ctcaaacccg acgatccctg cggtgaagat tgcaaaggcc
     5161 tcgaccattt ttaccaccaa cttcctaagt cgattccgct tccttccaaa ggaactatag
     5221 cacagtcatc ggtagcctgt cgaggaatcc ttaggagatt tgcacttttg cctaggttta
     5281 ttcacgatga tttcacagca gcttaggaat cttcggcgag tggcacgtgg ctcccccgcg
     5341 aaaagggccg gacgaaggtc cggccccctc gactgcttac gtttcgtaag acctaggcgc
     5401 ccatcccgag tcgctgggac tgctggaggg ccttgccctc ggtcttgagg gccggagcga
     5461 ccatgacctc ggcctactgg aactccttga tgttggcgta gccgcacgtc gcgaggctcg
     5521 tccgcagggc tccgaagagg ttgaggcggc cgtcgttctc gtgcgccggc ccgaccagga
     5581 cctcctccat cgagccgagc gtcgacgtct tgacccgggc cccgcgcggg agcgtcgggt
     5641 ggaacgtcgc catcccccag tggtagccgc ggcccggtgc ctcggcggcc gtcgccagcg
     5701 gggacccgat catcacggcg tcggctccgc aggcgatcgc cttggcgatg tcaccgccgg
     5761 tgcgcatccc gccgtcggcg atcacgtggc agtagcggcc ggtctcgtcg aggtggcgga
     5821 tgcgggcccc ggcggcgtcc gcgatcgcgg tcgcctgggg gacgccgagg ccgaggacgc
     5881 cccgagtggt gcaggccgcg ccggggccga cgccgacgag cacgcccatc gccccggtcc
     5941 gcatgaggtg cagcgccgtc gagtacgacg cgcacccgcc gacgattacc gggagctcgt
     6001 acgtcgagat gaaacgtttg aggttgagcg gctcgtgccg ggtcgacacg tgctcggccg
     6061 acacgaccgt cccctggatc acgaggatgt cgagctcggc ctcgagcgcg atcttgttcc
     6121 actgctcgac cttctgcggg gtcagcgagg ccgacgccgt gacgccggcg tccttgatct
     6181 cgcggattcg cttgtagatc agctcttcct tgaccggctc gccgtagatc tcctgcatgc
     6241 gggcggtcgc cttctccgag gggagctcgg cgatctcggc gaggatcgag tcggcgtcgt
     6301 cgtagcgggt ccacaggccc tcgaggttca ggaccgcgag gccgccgagg cgcccgatct
     6361 cgatcgccgt cgccggcgag acgacgccgt ccatggccga ggcgagcaag gggagctcga
     6421 accggaacgc gtcgatctcc caggagatgt cgacgtcgtc ggggtcccgg gtcctgcggg
     6481 acgggacgat tgcgatgtcg tcgaagccgt aggccctgcg accggacttc ccgatgccga
     6541 tctcgatctc cacaagctct cccttggttc gtcgcgtttg gaagcttcca acctatcggg
     6601 gccgccttca ttccgcccgg tggtccggtg aggaaattcc gcgctctggg gtccgctccc
     6661 cgagggagcc ggatcgctcc cggggcggcc ggcgatcctc cacatctcgg cctgtggcgg
     6721 gcgtcacagg gtctcgggcc tccaccgcgt cgcttgcagg tgttagcgtg tccgccggag
     6781 aaagacggag ggaccgcctc ggcgggaccc cccggggcaa gacggaggga ccgcctcggc
     6841 gggacccccg aggcaagacg gagcgggcag ctccacgtgc ggcggctcga gcaacgggcc
     6901 cgcctgcatc gaccggcggc cggacgaact ccccggccgg gaagacggct cttcgtagag
     6961 ccggcggcag gcactcattg gaggttggca ttggaacggt tgagcttcgg cgcgcgagcg
     7021 cgtcgcaagg ccaggttgaa gcgcctggca ccgtggttgt gggtagcgag cgtcctcatg
     7081 atggtgatgg gcgtccccgg cgcgaacgtg ggcgccgacg cgctccagcg gctgagcggg
     7141 ttcgaccgct ccgagctgcg gggcatcgag acgagcgaga gcgcggcggg gctgatgaag
     7201 ttccgcgggg ggacgttcca gtcccggccc gcggccacgc ccgagccgac tccgcccccg
     7261 acccccacgc ccgaggtcgt ggtcccggct gcgccggttg ctccggtcgc cccggcagtg
     7321 gaggtcgcgc ctccggcccc cgagggctcg gtgacggcga tcatctacgc ggccgccgcg
     7381 gagtacgggc tgagcggccc gtacctgctg tcggtcgccg cgtgcgagtc cggcctgaac
     7441 ccgtcggcag tgaactccgc cggctactac gggctgttcc agttctcgag cagcacatgg
     7501 gcgtcgaacg ggtacggctc gatctacgac ccggtcgccc aggcccgcac ggccgcccgc
     7561 atgatcgcgg ggggcggcgc cggccactgg ccgagctgcg cctagccctc catccggggt
     7621 agctcggggc ccggcgcccc gagcggcagc cggggctcga ccatcttggg gcgacccccg
     7681 agcagcacga tcgcggccgc gacggcgccg atgaccccgc acgccagcca cgcgccgtcg
     7741 ggggccgctg cgaagaagcg ggccccgacg ctgggcgcca gcatgagccc gagcgaccac
     7801 gtcaggcccc acgcgcccat gtagcggccg cgcatgtgct cgggagcgag ctcggccacg
     7861 taggccgacg agaccggggc gtcgatgacc tcgccgatcg tccacaggac caccgtggcc
     7921 gcgagcagcg gcatcgtgcc ggcgaacgcc gtcaacgcga agccgagccc cgtcacgaag
     7981 aagccgacgg cgatcacgcg ccgtgcgtcg agccgctgcg tcacggacgc gaccgggagc
     8041 tcgaggaaga cgacgagcag cccgttcaac gagaccagca agccgtagtc ggacgtcgac
     8101 aacccctcgg ccttcacgtg cagcgcgagc accgacatgg actgcaggta gacgtacgtg
     8161 atgccgaccg acgcgacgag gaaggcgacg aaggcccggt gacggagcat cgagcggatc
     8221 gaacctccgg tcgcatgggg gccgggcgcg ggcgggcgcc cccgcgggag gctcacgagc
     8281 gcgacggtcc cgaacacgac cgacgtgagc gcgtcaccga cgaagagcca gaagaacgag
     8341 cggtcggcga ggaagccggc ggcggcgggc ccgagcgcga accctgcgtt gacggcgagc
     8401 ctccacacgg cgaaggcggc gacgcgctgc tccggcggga cgaggtcggc gagcagcgcg
     8461 ctggacgcgg gccggtagag ctcggacgag agcccgatca ggaacacgaa gaacgtgatc
     8521 gcggcgagcc cctgtgcctg cgacagcgcg agcatcgcgg cggccgccga gtacatcgac
     8581 agcacgatcg tctcgcgccg tccgatgcgg tcggtgaggt atccgccgat ggcggaggcg
     8641 gcgaggtggc ccacgccgta cgcgccggcg gccgtcccgg ctgcggcggc cgagtagccc
     8701 tcggcggtca ggtagaggac gaggaagacc gagacgaagc tgccgaaccg gttgacgaag
     8761 gcgccggcgc agacgatcca cgcggcgcgc gggagctcac gcacgttctg tcgcagggtc
     8821 ctcggcgagt cgctcatcgc cgagaagtga accagctcgg tgtgacggtt cttggagaga
     8881 cggccgatat agcctccgag gcgtgtccgc gaccgacccg agaaccaggt ggggactcgt
     8941 cttgcacgta gtcgtcgcct tgtcgctgtg gtcctggttc gacgtacccg tggatcccgc
     9001 cggcgagagc gggctggcgg ccacgctcct cgtcctgatc acggcctccg tgccgttcgc
     9061 gctcctcttc gccgcgctcg gcgctcgctt cgcggagcgc acctccgtga cgcgggtggt
     9121 tgcgtgggtg ggcgccctcg tctacggcat ctggctggga gggtgggcct ctgtggtggg
     9181 gacgtcgggg ggagagctgg agtgcgaccc acccgactgc cgcagctcct gggggccgcg
     9241 cgtcctcacg ttcgtgaccg cgacgatcat agtcctcgtc tgcgcgcgga tcgaggcggc
     9301 cctacggaag aggtggtcta gcgagccttc atgaagaagt gctcgtggcc cggctcggtc
     9361 ggccagtccc cgacctcctc ccagtacttg cgcacgggtt ccggcgcgtc gtcgatcgag
     9421 atctcctcga agccgaagtg cgaatagaag cgcatccggt tgtcgtggca gcagaggtac
     9481 atcgtgcccc cgcggctgtt catcgccgtc tgcatcagct gccggccgat gcccttctcg
     9541 cgccggccct ctttgacgag gacaccgtcg acaacgagcg tgttcggctc gacctcgacc
     9601 agacgcagcg cgccgacgac gccgccgtcg cgcgcaatga aggccttgcc cttgtcgagg
     9661 ttctcgtcgc cccagccgta ctcctcgtag agctctgcga tgtcggctct gtcgtgtcgc
     9721 gtggccctcg tgatgaacat cgctatgcga ctccttgcag ctctcgtgcg gcggagcgca
     9781 attcttccaa gaccgcgtct gggacctcga ccccgtgctt gccgccggga agaccacggt
     9841 cgggacgcat gtcgccgtgg tagtcgctcc cgcccgagac gatcaggccc ttcgctcggc
     9901 cgagagcgcg gtacggctcc tgctcgatct ccggcaggtc gccgtggtag ccctcgatcc
     9961 cgcgcagccc gacggagacc agctcgtcga cgaacgtctc gagctgctcg atcgggagct
    10021 tcagggtgaa ggggtgcgcg agcaccggca cgacgccgga gcggaccatg agctcggtcg
    10081 cctcgaccgg cgagtacagc ttgcgcgtga ggtacgcctt cgcgcccttg gcgaggtact
    10141 cgtcgaaggc ggtctggtag tcggggaccg cgccgtggcg cacgagcacc gcggcgaagt
    10201 gcggacggcc tatctgggcc cccggccccc ccgcctc
//
LOCUS       DASQDO010000033         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1106347, whole genome shotgun sequence.
ACCESSION   DASQDO010000033 DASQDO010000000
VERSION     DASQDO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106347"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VEV43_01065"
     CDS             complement(<1..659)
                     /locus_tag="VEV43_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP22140.1"
                     /translation="MRIPFARALTQRDFALLWLGQTTSLVGDGIFNVALAWQALQLPD
                     GAKVLGAVLLVRSAARVALLLVAGALADRYEKRLLVLGGDTLQMVAVGWLAYVVGTGD
                     LHTWQLVTVAGVTGAGSAIFLSASTAIVPELVDEDYYQSANSLRSTSVVLSDLVGSAA
                     GGLIVATAGIATAFTVNAATFVASITALLFVRPRRRAVAETEENVFSQVKEGLRYVVR
                     TP"
     gene            complement(683..2029)
                     /gene="ccrA"
                     /locus_tag="VEV43_01070"
     CDS             complement(683..2029)
                     /gene="ccrA"
                     /locus_tag="VEV43_01070"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HYP22141.1"
                     /translation="MPGPIDQLRTAIVEGAPGEEIAALESPESVRAVFTRKDEQDMFE
                     GMESSAKDPRKALHVDEVPLPPLGPNECHVAVMASAINFNTVWTSIFEPVSTFAFLEK
                     FGRESDLGKRHDLDYHVVGSDAAGVVLRTGPGVTRWKPGDHVTVHCNYVDMEAHEGHD
                     DSMMDPSQRIWGFETNFGSLSEITMVKANQLMPKPAHLTWEEAACLGLVSSTSYRMLV
                     SPNGANMKQGDVVLIWGATGGLGGFACQYVLNGGGIPVGVVSSPDKCELLNAIGVEHV
                     IDRKAEGYAFWDGDRPNQKEFRRFGKKIRELAGEDPDIVFEHPGKQTFGASVFVTKRG
                     GKIVTCASTSGYEHVYDNRYLWMHLKSIIGSHFANYKEAWEANRLAALGMIHPILSQT
                     YSLEDTGEAAYQVHQNLHKGKLGILVLAPEEGGGVTDEEKRSRHVKEIDLFKKFSD"
     gene            2162..3322
                     /locus_tag="VEV43_01075"
     CDS             2162..3322
                     /locus_tag="VEV43_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP22142.1"
                     /translation="MSEGLSFRLTEEQEAFRQSVRQLVEDKIAPRAAEIDESDEYPWD
                     IDELMVKNGFAGVSYPEEYGGAGGGAVELCLLVEEVSRWSGGVALVTAVNKLGAIPVL
                     LAGSDDMKKKVCTGITNGEHRMSYCLTEPNSGSDAAGMKSRAVRDGDDWILNGSKRFI
                     TGAGTSDLYTYFAVTNPDAPKGKNITAFLVSKDMEGFSVGRKEDKMGIKGSPTAEVIL
                     ENCRVPSENVIGEVDKGFQIALRTLDFSRPTVAAQALGIAQGAFDFAVGYCNERQQFG
                     KPVSAFQGMQFMFADMAMAIETARFAVYRAAVAVDEGAADVSYWAAIAKCYASDVAMR
                     VTTDCVQALGGYGYIKEYPVERMMRDAKITQIYEGTNQIQRVVIARSIFGTR"
BASE COUNT          539 a         1098 c         1190 g          540 t
ORIGIN      
        1 ggcgtccgca cgacgtagcg caggccctcc ttcacctgcg agaagacgtt ctcctcggtc
       61 tccgccacgg cccgccggcg aggccggacg aagaggagcg ccgtgatgct ggctacgaag
      121 gtcgcggcgt tcaccgtgaa cgcggtcgcg atccctgcgg tggccacgat cagccccccg
      181 gccgccgagc cgaccaggtc gctcaggacc acgctcgtgc tccgcagcga gttcgcggac
      241 tggtagtagt cctcgtctac gagctcgggg acgatcgcgg tcgaggcgga caggaagatc
      301 gcgctgccgg cgcccgtgac gccggcgacg gttacgagct gccaggtgtg caggtcgccg
      361 gttcctacga cgtaggccag ccatcccacc gcgaccatct ggagggtgtc gccgccgagc
      421 accaggaggc gcttctcgta ccggtccgcg agcgcgcccg ccacgagcag caacgctacg
      481 cgtgcggcgc tccggacgag caggaccgcg ccgaggacct tcgcgccgtc ggggagctgg
      541 agggcctgcc acgcgagcgc gacgttgaag atgccgtcgc cgacgagcga ggtcgtctgg
      601 cccagccaca ggagagcgaa gtcgcgctgc gtcagcgcgc gggcgaacgg gatccgcatc
      661 gccggagcct agtgaggctg cgtcagtcgc tgaacttctt gaagaggtct atctccttga
      721 cgtggcgcga ccgcttctct tcgtcggtca cgcccccgcc ctcttccggc gccagcacca
      781 ggatcccgag cttgcccttg tgcaggttct ggtgcacctg gtacgcggcc tcgccggtgt
      841 cctccagcga atacgtctgc gacaggatcg ggtggatcat gccgagggcc gcgaggcggt
      901 tcgcctccca cgcctccttg tagttggcga agtggctccc gatgatcgac ttgaggtgca
      961 tccacaggta gcggttgtcg tagacgtgct cgtagcccga cgtcgacgcg caggtgacga
     1021 tcttgccccc gcgcttcgtg acgaagacgc tcgcgccgaa cgtctgcttg ccggggtgct
     1081 cgaagacgat gtcggggtcc tcgccggcga gctcacggat cttcttgccg aagcggcgga
     1141 actccttctg gttcgggcgg tcgccgtccc agaacgcgta gccctcggcc ttgcggtcga
     1201 tgacgtgctc gacgccgatg gcgttcagca gctcgcactt gtcgggtgac gacacgactc
     1261 cgacggggat gcccccgccg ttgaggacgt actggcacgc gaagcctccg agcccgccgg
     1321 tcgcgcccca gatcagcacg acgtcgccct gcttcatgtt ggcgccgttg ggcgacacga
     1381 gcatccggta cgaggtcgac gagacgaggc cgaggcacgc ggcctcctcc cacgtcaggt
     1441 gcgcgggctt cggcatgagc tggttcgcct tcaccatcgt gatctcggac agcgagccga
     1501 agttcgtctc gaagccccag atgcgctggc tcgggtccat catcgagtcg tcgtggccct
     1561 cgtgggcctc catgtccacg tagttgcagt gcacggtgac gtggtcgccg ggcttccagc
     1621 gcgtgacgcc gggcccggtg cggagcacga cgcccgccgc gtcggatccg acgacgtggt
     1681 agtcgaggtc gtgacgcttg ccgagatcgc tctcgcggcc gaacttctcg aggaaggcga
     1741 acgtcgagac gggctcgaag atcgaggtcc agacggtgtt gaagttgatc gccgaggcca
     1801 tgaccgcgac gtggcactcg ttcgggccga gcggcgggag cgggacctcg tcgacgtgga
     1861 gggccttgcg ggggtccttg gcgctcgact ccatgccctc gaacatgtcc tgctcgtcct
     1921 tgcgcgtgaa cacggcgcgg acggactcgg gcgactcgag ggcggcgatc tcctcgccgg
     1981 gggcgccttc gacgatcgcg gtccgaagct ggtcgatggg tccgggcacg gtgggggcct
     2041 cctactgggt gtcttcgatc ttctcttgga gcgtcgccga ggcgacgctt cggggggaag
     2101 gccgcagcct aatggaacta gaccgcccgg tcgggttcca gggggccggt accatgggcc
     2161 tatgagcgaa ggactcagct tccgcctcac cgaggagcag gaggctttcc gccagtccgt
     2221 gcggcagctc gtcgaggaca agatcgcgcc ccgggccgcc gagatcgacg aatccgacga
     2281 gtatccgtgg gacatcgacg agctcatggt gaagaacggg ttcgcgggcg tctcctaccc
     2341 ggaggaatac ggcggcgcgg gtggcggcgc ggtcgagctc tgccttctcg tcgaggaggt
     2401 ctcccggtgg tcgggcgggg tggccctcgt caccgccgtc aacaagctcg gcgcgatccc
     2461 ggtcctcctc gccggcagcg acgacatgaa gaagaaggtc tgcaccggca tcacgaacgg
     2521 cgagcaccgg atgtcgtact gcctgaccga gccgaactcg ggctcggacg ccgccgggat
     2581 gaagagccgg gcggtccggg acggcgacga ctggatcctc aacggctcca agcggttcat
     2641 cacgggggcc gggacctccg acctctacac gtacttcgcg gtgacgaacc ccgacgcgcc
     2701 gaagggcaag aacatcacgg cctttttggt gtcgaaggac atggagggct tctccgtcgg
     2761 ccgcaaggaa gacaagatgg ggatcaaggg atcgccgacc gccgaggtga tcctcgagaa
     2821 ctgccgggtg ccgagcgaga acgtgatcgg cgaggtcgac aagggcttcc agatcgcgct
     2881 ccggacgctc gacttctcgc ggcccacggt cgcggcgcag gctctaggga tcgcccaggg
     2941 cgcgttcgac ttcgcagtcg gctactgcaa cgaacgacag cagttcggca agccggtctc
     3001 ggcgttccag gggatgcagt tcatgttcgc cgacatggcg atggcgatcg agacggcgcg
     3061 cttcgccgtc taccgggccg cggtcgcggt ggacgagggc gccgcggacg tctcctactg
     3121 ggcggcgatc gcgaagtgct acgcatccga cgtagcgatg cgggtgacca cggactgcgt
     3181 gcaggcgctg ggcggctacg gctacatcaa ggagtacccg gtcgagcgca tgatgcgcga
     3241 cgcgaagatc acccagatct acgagggcac gaaccagatc cagcgagtcg tgatcgctcg
     3301 cagcatcttc ggcacgcggt aggcgaggcc ggagtcgccg tgcagtcacg cccgaaggcg
     3361 ggcggag
//
LOCUS       DASQDO010000034         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_556129, whole genome shotgun sequence.
ACCESSION   DASQDO010000034 DASQDO010000000
VERSION     DASQDO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556129"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VEV43_01080"
     CDS             complement(<1..635)
                     /locus_tag="VEV43_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYP22143.1"
                     /translation="MKDVGIVGMPYSGKSTLFQALTSAGSAGGRSNQAVVDVPDSRLD
                     VLAGIEKSKKVVHAKVRFVDVPGGLTAQGIAEFRNTDALCMVVRAFGPDANPKSELAS
                     LEAEMVLADLTNVESGLEKARTKARGSAEGKVEAAVLEKAHELLNAERPLRDGTFDDA
                     ERKVLRGFGPLTMKPWIVVANTEEGSGLPEGLPSDAFPVSAALEAEVAGMD"
     gene            648..1433
                     /locus_tag="VEV43_01085"
     CDS             648..1433
                     /locus_tag="VEV43_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP22144.1"
                     /translation="MVPPRETGLNGKGVLVTGGAGGIGSALCRTFAGEGAKVGIHYNT
                     SSERAEKLAAETGGVPFQADLTDEAAVDALFRDAVERLGRLDVLVANAGKWPSADEPV
                     WTMGLDRWRATIAANLDSVFLSCRAFLRHVESTQTGSIVIVSSTAGLIGEAGHSDYAA
                     AKSALAGGFLKSLKNEITHIAPRGRVNVVAPGWTETEMARDTLAEPGFVDRITRTMAL
                     RKVGQAEDVAGVVVALASDEISGHVTGEVVTVAGGMEGRVLRE"
     gene            complement(1434..>1766)
                     /locus_tag="VEV43_01090"
     CDS             complement(1434..>1766)
                     /locus_tag="VEV43_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22145.1"
                     /translation="RQSYHVGAYAIVRTDVPEIFLAEDADAISRALAIHLVAQLPAAE
                     VSSPSRLRDMREALLEERWADALVEWIDETDVAVDVYDEPTKVWSTDELDLDRASLEI
                     RFAPLFAD"
BASE COUNT          279 a          620 c          605 g          262 t
ORIGIN      
        1 tcgtccatcc cggcgacctc ggcttcgagc gcggcgctca cggggaacgc gtcggacggg
       61 aggccttccg gcaaaccgct gccctcctcg gtgttcgcga cgacgatcca cggcttcatc
      121 gtgagggggc cgaagccgcg cagcaccttg cgctccgcgt cgtcgaacgt cccgtcgcgc
      181 agcggccgct ccgcgttcaa cagctcgtgg gccttctcga gcacggcggc ctcgaccttg
      241 ccctccgccg agccgcgcgc cttcgtgcgc gccttctcga gaccactctc gacgttcgtc
      301 aggtcggcga ggaccatctc ggcttcgaga gacgcgagct ccgacttcgg gttcgcgtcg
      361 ggcccgaacg ctcgcacgac catgcacagc gcgtcggtgt tacggaactc cgcgatcccc
      421 tgcgcggtca agcctccggg cacgtcgacg aagcggacct tcgcgtggac gaccttcttg
      481 gacttctcga tccctgcgag gacgtcgagc cgggagtcgg gcacgtcgac caccgcttgg
      541 ttcgagcggc cgccggccga gcccgcgctc gtgagcgcct ggaacagcgt cgacttcccc
      601 gaatacggca tccctacgat gcccacgtcc ttcatgggcg acaacgtatc gtgcccccga
      661 gggagacagg gctgaacggc aagggcgtcc tcgtcaccgg gggcgccggc gggatcggga
      721 gcgcgctgtg ccgcacgttc gcgggcgagg gcgcaaaggt cggcatccac tacaacacga
      781 gctcggagcg cgccgagaag ctcgcggccg agaccggagg cgttccgttc caagccgacc
      841 tcaccgacga ggccgcagtg gacgcgctct tccgcgacgc ggtcgaacgg ctcggccgcc
      901 tcgacgtcct cgtcgccaac gcagggaagt ggccgagcgc ggacgagccc gtgtggacga
      961 tggggctcga ccgctggcgc gcgacgatcg ccgccaacct cgacagcgtg ttcctgtcgt
     1021 gccgcgcgtt cctccgccac gtggagtcga cgcagaccgg cagcatcgtg atcgtctcgt
     1081 cgaccgcggg attgatcggc gaggccggcc actcggacta cgcggccgcc aagtccgcgc
     1141 tcgccggcgg cttcctcaag agcctcaaga acgagatcac ccacatcgcg ccgaggggcc
     1201 gggtcaacgt cgtggcgcct ggatggaccg agacggagat ggcccgcgac accctggccg
     1261 agcccggctt cgtcgaccgg atcacgagga cgatggcgct gcggaaagtg ggccaggccg
     1321 aggacgtggc cggggtcgtg gtcgccctgg cgtcggacga gatcagcggg cacgtgacgg
     1381 gcgaagtggt gacggtggcc ggcgggatgg agggccgggt cctccgcgag tagtcagtct
     1441 gcgaacaagg gcgcaaaacg tatctcgagc gacgcccgat cgagatcgag ctcgtcggtc
     1501 gaccacacct tcgtaggttc gtcgtacacg tctacggcga cgtcggtctc gtcgatccat
     1561 tccacgagcg catcggccca tcgttcctcc agcaacgcct cccgcatgtc gcgcaggcga
     1621 gacggcgacg agacctcggc cgcggggagc tgcgccacga gatggatcgc caaagcgcgc
     1681 gatatcgcgt cggcgtcctc cgctaggaag atctcgggta catccgtccg cacgatcgcg
     1741 tacgcgccca cgtggtagct ctgacg
//
LOCUS       DASQDO010000035         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_372706, whole genome shotgun sequence.
ACCESSION   DASQDO010000035 DASQDO010000000
VERSION     DASQDO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372706"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEV43_01095"
     CDS             <1..295
                     /locus_tag="VEV43_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HYP22146.1"
                     /translation="QEARVRQLRQMIENAEIIETSDDGVVRPGMIVTLRYEGEEPETY
                     LFGLREEKGGEHPVLTPDSPLGQALTGHAAGDTVKTKVPAGELKIEIVEVRAP"
     gene            complement(292..1350)
                     /locus_tag="VEV43_01100"
     CDS             complement(292..1350)
                     /locus_tag="VEV43_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYP22147.1"
                     /translation="MAATTLIRNVSVLWGPDLALVERTTLAIEGRRVAGLDGGAAPAG
                     AETVDGGGLLLVPGFVDAHVHIGFADPAEVLRRGVTTVRDLGWPPEEIFPLARASEQP
                     GFAGPLIRAVGPIHTASGGYPTQASWAPAGTGCEVAGESDAKAAVRRLDMGAAAVKFA
                     LNPPVGPVLDHDTLRGLVDAAHAAGLRATGHVYGLAELQKALDCGVDELAHMLMSEEE
                     IPDAVIVRMAGAGMTVVPTLSIRFGRDRELAIDNLARFRAAGGRVVYGTDLGNEGPGP
                     GIDRTEVTAMTAAGFDSLGVVAAATVDAARHLGLDSKGVLGDGMDADVVAVPLDALER
                     PELLSDVKMVWREGGRAS"
     gene            1592..2731
                     /locus_tag="VEV43_01105"
     CDS             1592..2731
                     /locus_tag="VEV43_01105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYP22148.1"
                     /translation="MTEEVTFDTLGIPRDVLARLKKRGFDRPFPIQARVLPFALAGRD
                     VFGQAQTGSGKTLAFAVPIVAAATRKGQNPYALVMVPTRELAAQVAEEIDGISGGLRV
                     MAAYGGRPVAKDVAKLNRGIDVVVATPGRLADLYERGAMVLDDVSILVLDEADRMLDL
                     GFMYDMDWLIRRLPAQRQTMLFSATLATTIRQLARRYMTDPEHVDVAEEMTQVEEIDH
                     RFFLVHPMDKLQVLLALVAKQPLTIVFTRTKRYAARLNDELKRGGIAVAALHGDMPQG
                     ARTKALADFTAQKAKVLVASDVAARGLDVAGVEQVINYDPPDDPTSYTHRAGRTARAG
                     ATGLCVTLCTSGEKGHVELMLKRLGLDATIEEVFSTSPVLKEMAS"
     gene            2747..3085
                     /locus_tag="VEV43_01110"
     CDS             2747..3085
                     /locus_tag="VEV43_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22149.1"
                     /translation="MEPGEVIWEGKIGTTNLRVGVPHSEAIIAEWDTPKGSRHKVSDS
                     IQEFERAVLFQLLLTSGDVDEKVTADVIAAVEKAQAERPKRVSEDAPRRRKNPKARQH
                     RRSRRPPRRR"
BASE COUNT          507 a         1141 c         1153 g          446 t
ORIGIN      
        1 gcaggaggcg cgcgtccgcc agctccgcca gatgatcgag aacgccgaga tcatcgagac
       61 ctccgacgac ggcgtcgtgc gtccggggat gatcgtgacg ctgcggtacg agggcgagga
      121 gcccgagacg tatctgttcg ggttgcgcga ggagaagggg ggcgagcatc cggtgctcac
      181 gcccgactcc ccgctcggcc aggcgctcac cgggcacgcg gcgggcgaca ccgtcaagac
      241 gaaggtcccg gcgggggagc tcaagatcga gatcgtggag gtccgcgctc cttaggacgc
      301 gcgcccgccc tctcgccaca ccatcttcac gtcgctcagc agctcgggac gttcgagcgc
      361 gtcgagcggg accgcgacga cgtctgcgtc catcccgtcg cccagtactc ctttcgaatc
      421 cagcccgaga tggcgcgcgg catcgacggt cgccgccgcg accaccccga gcgaatcgaa
      481 gccggccgcg gtcatggccg tcacctcggt gcggtcgatg ccgggcccgg ggccctcgtt
      541 gccgaggtcc gtcccgtaga cgacgcgccc gccggcggcg cggaagcggg caaggttgtc
      601 gatcgcgagc tcgcggtcgc gcccgaacct gatcgacagc gtcgggacga cggtcatgcc
      661 ggcgccggcc atccggacga tgaccgcgtc cgggatctcc tcctcgctca tcagcatgtg
      721 cgcgagctcg tcgacgccgc agtcgagcgc cttctggagc tccgcgaggc cgtacacgtg
      781 tccggtagcg cggaggccgg cggcgtgcgc ggcgtcgacc aagccccgaa gcgtgtcgtg
      841 gtccagcacc ggaccgaccg gagggttgag cgcgaacttg accgcggccg ctcccatgtc
      901 gagccgtcgg acggccgcct tcgcatcgga ttcgcccgcg acttcgcagc ccgttcccgc
      961 cggcgcccac gatgcctgcg tgggatatcc gccggaggcc gtgtggatcg ggccgaccgc
     1021 tcggatcaga ggcccggcga acccgggctg ctcggacgcg cgggccagcg ggaagatctc
     1081 ttcgggcggc catccgaggt cgcggacggt cgtaaccccg cggcgaagga cctcggccgg
     1141 gtccgcgaag ccgatgtgga cgtgagcgtc gacgaacccg ggaacgagca gcaagccccc
     1201 gccgtcgacg gtctcggcgc cggccggggc ggcccctccg tcgagccccg cgacgcgtct
     1261 tccctcgatc gcgagcgtcg ttcgttcgac gagcgcgaga tccggccccc acagcacgga
     1321 gacgtttcga atgagcgtcg tagcggccat cgcagtgcat attcggccat ccggggcggc
     1381 ggcgggtaga ctcggctgcg taacgcccac gcagcgccgg cctcggcgcc tccctcgacg
     1441 catcgtttcg gcggtgcgcc acaaacaggt ggcagggccc gtccctcgcc gcgcgggcgg
     1501 aggcaacggc ctcggccggc gccaaacgga gatggggcct gtgcccccga gatcaaaaag
     1561 gaatggtgac gtcctggaac aggcagaccc gatgaccgaa gaggtcacgt tcgacactct
     1621 cggcatcccc cgggacgtcc tcgcgcgcct caagaagcgc ggcttcgacc gtccgttccc
     1681 gatccaggcc cgggtcctcc cgttcgccct cgccgggcgc gacgtcttcg gccaggccca
     1741 gacgggctcg gggaagaccc ttgcgttcgc ggtcccgatc gtggcggcgg cgaccaggaa
     1801 gggacagaac ccgtacgcgc tggtgatggt cccgacgcgt gagctcgcgg cccaggtcgc
     1861 cgaggagatc gacgggatct ccggcggtct gcgcgtgatg gcggcgtacg ggggccggcc
     1921 ggtggcgaag gacgtggcga agctcaaccg cggcatcgac gtggtcgtcg cgacgcccgg
     1981 ccgtctcgcg gacctctacg agcgcggcgc gatggtcctc gacgacgtct cgatcctcgt
     2041 gctcgacgag gccgaccgca tgctcgacct cggcttcatg tacgacatgg actggctcat
     2101 ccggcggctc ccggctcagc gtcagacgat gctcttctcg gcgacgctcg ccacgacgat
     2161 ccggcagctg gcgcgccgct acatgaccga tcccgagcac gtcgacgtcg ccgaggagat
     2221 gacgcaggtc gaagagatcg accaccgctt cttcctcgtc cacccgatgg acaagctcca
     2281 ggtgctgctg gcgctcgtcg cgaagcagcc gctgacgatc gtgttcacga ggaccaagcg
     2341 ctacgcggcc cggttgaacg acgagctgaa gcggggcggc atcgccgtcg cggccctgca
     2401 cggcgacatg ccgcagggcg cgcgcacgaa ggcgctggcg gacttcaccg cgcagaaggc
     2461 gaaggttctc gtcgcgtccg acgtcgcggc gcgggggctc gacgtggcgg gcgtcgagca
     2521 ggtgatcaac tacgacccgc cggacgaccc cacgtcgtac acgcaccgcg ccggccgcac
     2581 ggcccgcgcc ggcgccacgg ggttgtgcgt cacgttgtgc acgtcaggcg agaagggcca
     2641 cgtcgagctg atgctgaagc gtctcgggct cgacgcgacg atcgaagagg tcttctcgac
     2701 gtcgccggtc ctcaaagaga tggctagttg agaaggggct gaggcgatgg aaccgggcga
     2761 ggtcatctgg gaagggaaga tcggcacgac gaacctgcgc gtgggcgtac cgcattccga
     2821 ggcgatcatc gccgagtggg acacgccgaa gggcagccgc cacaaggtct cggactcgat
     2881 ccaggagttc gagcgggccg tgctgttcca gctcctgctg accagcggcg acgtcgacga
     2941 gaaggtgaca gcggacgtga tcgcggcggt cgagaaggct caggccgagc ggccgaagcg
     3001 cgtctcggaa gacgccccgc gccggcgcaa gaacccgaag gccaggcagc accgccgctc
     3061 gcgccgcccc ccgcgccggc gttagctacc caccccgcgg gggtgtagga cttcccctgt
     3121 gaacaccgcc gtccgttatc cggaatccgt ccgcgctgtt ctcgccgcgc tggcgcgcgg
     3181 cgtcctcggg cgcgagcacg cgccgggtct gatcccgacg atgctgcagg tcgtcgacat
     3241 gctcccc
//
LOCUS       DASQDO010000036         7984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_495036, whole genome shotgun sequence.
ACCESSION   DASQDO010000036 DASQDO010000000
VERSION     DASQDO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495036"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..177
                     /gene="rpmJ"
                     /locus_tag="VEV43_01115"
     CDS             64..177
                     /gene="rpmJ"
                     /locus_tag="VEV43_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYP22150.1"
                     /translation="MKVQPSVKKVCDKCKIIRRRGRVMVICSNPRHKQRQG"
     gene            187..564
                     /gene="rpsM"
                     /locus_tag="VEV43_01120"
     CDS             187..564
                     /gene="rpsM"
                     /locus_tag="VEV43_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYP22151.1"
                     /translation="MARIAGVDLPREKRLDIGLTYIYGIGRTKALETCIGARVDPSTK
                     VRDLTDEEVVSIRDWIGNNYRVEGDLRREVNQHIKRKVEIGSYQGLRHRRGLPVHGQR
                     THTNARTRKGPKKTVAGKKKAKK"
     gene            622..1014
                     /gene="rpsK"
                     /locus_tag="VEV43_01125"
     CDS             622..1014
                     /gene="rpsK"
                     /locus_tag="VEV43_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYP22152.1"
                     /translation="MAAPKKKKQRRREKKNVVHGVAHIKSTFNNTIIAISDLEGNVLS
                     WASAGNVGFKGSRKSTPFAAQLAAEAASRRAQEHGVRRVDVEVKGPGSGRETAIRSLQ
                     AAGLEIIGIKDVTPVPHNGCRPRKRRRV"
     gene            1015..1641
                     /gene="rpsD"
                     /locus_tag="VEV43_01130"
     CDS             1015..1641
                     /gene="rpsD"
                     /locus_tag="VEV43_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYP22153.1"
                     /translation="MARYTGPVCKLCRREKTKLFLKGTRCESPKCPIEKGRPPPGEHG
                     RGRVRESEYLLQLREKQKAKRFYGILEKQFRGYYEEAANSKGVTGEELMRICESRLDN
                     VVYRAGFAMNRPMARQLVSHGHFEVNGRKVNIPSFRVKPGDVVTVRNRSKAMGRIVEN
                     TSYAEGREIPDWLGSNLREMRVEVRALPERGQMDIPVQEQLIVEYYSK"
     gene            1681..2598
                     /locus_tag="VEV43_01135"
                     /pseudo
     CDS             1681..2598
                     /locus_tag="VEV43_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
     gene            2617..3285
                     /gene="rplQ"
                     /locus_tag="VEV43_01140"
     CDS             2617..3285
                     /gene="rplQ"
                     /locus_tag="VEV43_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HYP22154.1"
                     /translation="MPAPKKGPRLGSNPSHQALMLSNLALSLFEHERVKTTEAKAKLL
                     RPYAERLITKAKKGDVHNRRQVLSTIEDRAVVHKLFADIAPRFADRNGGYTRILKLGP
                     RNGDGAPMALIELVESATVTTVESEPDEGGKRRRLPRPRRRGASADLPQDKPVRSRAA
                     DAAAAGAAAAVGGDDEDEGTEDTGADEAAAEEATAEAEEAGAPDDDAPSAGGAATEND
                     DSSN"
     gene            3282..4052
                     /gene="truA"
                     /locus_tag="VEV43_01145"
     CDS             3282..4052
                     /gene="truA"
                     /locus_tag="VEV43_01145"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYP22155.1"
                     /translation="MNLRLDLAYDGAPFRGFARQPGQKTVQGDLEDALTRVLRATPVL
                     TVAGRTDAGVHAVGQVVSADGVPDDADPEGLRGSLNSICGPSIVVHGCVRAPDEFNAR
                     FDARSRTYVYAISTEDVPDPWLGRTAWHHPGPLDLDAMSEAAGHLLGRRDFSSFGRLP
                     APDASPERTLYELTCSAAGSIVRIRARADAFIQQMVRSLVGTLVAVGEGKMEPDRMPS
                     VLAAKDRAAAGPVAPPHGLCLVSVEYETGWSRPFDPFE"
     gene            4122..4613
                     /gene="rplM"
                     /locus_tag="VEV43_01150"
     CDS             4122..4613
                     /gene="rplM"
                     /locus_tag="VEV43_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HYP22156.1"
                     /translation="MKTHVTKPNEITREWWIVDADGVVLGRLASEVAKRLRGKHKPSY
                     SPHLDTGDHVIIVNASKVVLTGSKLTDKQYHRHSGYPGGLRSVPYSKLMAERPDIAVE
                     KAVKGMLPSNRLGRAMLKKLKVYNGAEHPHSAQAPKPLDVKDVAAPHNPEQAHTVEEV
                     TGG"
     gene            4606..4998
                     /gene="rpsI"
                     /locus_tag="VEV43_01155"
     CDS             4606..4998
                     /gene="rpsI"
                     /locus_tag="VEV43_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYP22157.1"
                     /translation="MAEALVRATGRRKEAVCRARLLPGSGRWEINGRPIEDYFPSATH
                     RMIITEPLRLTETEGRYDIIAKIEGGGVSGQAGALRHSISKALVELDPDLRPVLKKAG
                     FLTRDAREKERRKYGLKKARKAPQYSKR"
     gene            5018..6361
                     /gene="chrA"
                     /locus_tag="VEV43_01160"
     CDS             5018..6361
                     /gene="chrA"
                     /locus_tag="VEV43_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HYP22158.1"
                     /translation="MTSPAPQRDLVPRREAFLWWLKLGFINFGGPAGQIAIMHDELVD
                     RRRWINDDRFLHALNFCMLLPGPEAQQLAIYVGWLLNGTLGGIVAGVLFVLPAFFLIL
                     GLSWTYAAHGDVTWIAAVFYGLCSAVIGLVAVAVIRVGSKALKTPAAWAVAALAFVTI
                     FFLDVPFPLIVLGAAIAGYVGSRIDPRWWPASGHGEEREGDHVISGAALERVRPTWRR
                     SVAVLLTGLAIWWGPLLLVLLWRGTEDTLSKEALFFSQAAMVTFGGAYAVLSYINEAA
                     VQQFGWLAPGQMVTGLGLAESTPGPLIMVTEFVGFLGGYNFPGGLDPVVAGILGATIT
                     TWATFAPCFLWIFLGAPYVERLRGKRGLSAALSAITAAVVGVILNLAVTFGAVALFDD
                     VDGSRGFPVPDPTSIDAFAAALAIASLVALWRYKVNVLWVVLASAGAGLLYRLVV"
     gene            6373..6717
                     /locus_tag="VEV43_01165"
     CDS             6373..6717
                     /locus_tag="VEV43_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22159.1"
                     /translation="MKWFGVRCVFWHDGDSFEERITLWRAESIDEAIAQAEEEARAYC
                     EGIDCKYVGLAQGYGPAAGPGGDDAVEIVPGTEVFSLFRLSEFEPREYLDHFFDTGSE
                     VHQDWTPGSFPS"
     gene            complement(6714..7850)
                     /locus_tag="VEV43_01170"
                     /pseudo
     CDS             complement(6714..7850)
                     /locus_tag="VEV43_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
     gene            7839..7984
                     /locus_tag="VEV43_01175"
                     /pseudo
     CDS             7839..7984
                     /locus_tag="VEV43_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
BASE COUNT         1326 a         2726 c         2721 g         1211 t
ORIGIN      
        1 gctgtccccg tacgacctca ctcggggtcg cgtcgtctac cgctacaagt agggggccgg
       61 gtcatgaagg tgcaaccgag cgtcaagaag gtctgcgaca agtgcaagat catccgccgg
      121 cgtggccgcg tgatggtgat ctgctcgaac ccgcggcaca agcagcggca aggttaagga
      181 gaaacattgg cacgtatcgc cggagtcgac ctccctcggg agaagcgtct ggacatcggg
      241 ctgacctaca tctacgggat cggccgaacg aaggcgctcg agacgtgcat cggcgcgcgc
      301 gtcgatccct cgacgaaggt ccgggacctg acggacgaag aggtcgtcag catccgcgac
      361 tggatcggca acaactaccg ggtcgagggt gacctccgcc gcgaggtcaa ccagcacatc
      421 aagcgcaagg tcgagatcgg ttcgtaccag gggctccggc accgccgcgg cctcccggtg
      481 cacggccagc gcacgcacac gaacgcccgg acccgcaagg gcccgaagaa gaccgtggcc
      541 ggcaagaaga aggccaagaa gtagtcacga aggatcgcct cggcgatcct ccaaggacag
      601 acgcacgagg aagggcgggt tttggcagct cctaagaaga agaagcagcg acgtagggag
      661 aagaagaacg tcgtgcacgg cgtggcgcac atcaagtcca cgttcaacaa cacgatcatc
      721 gcgatctccg acctcgaggg caacgtcctg tcgtgggcgt ccgcggggaa cgtcggcttc
      781 aagggctcgc gcaagtcgac cccgttcgct gcccagctcg cggccgaggc cgcgtcccgc
      841 cgggcgcagg agcacggcgt ccgccgcgtc gacgtcgagg tcaagggccc cggctccggc
      901 cgcgagaccg cgatccgttc gctccaggcg gcgggcctcg agatcatcgg gatcaaggac
      961 gtgacgccgg tgccgcacaa cggctgccgc ccccgtaaga ggaggcgcgt ctagatggcg
     1021 aggtacaccg gccccgtctg caagctctgc aggcgcgaga agaccaagct cttcctcaag
     1081 ggaacgcgtt gcgagtcccc gaagtgcccg atcgagaagg ggcgcccccc tcccggcgag
     1141 cacggcaggg gccgcgtgcg cgagtccgag tacctgctcc agctgcgcga gaagcagaag
     1201 gcgaagcgct tctacggcat cctcgagaag cagttccgtg gctactacga ggaggccgcg
     1261 aactccaagg gcgtcacggg cgaggagctc atgcgcatct gcgagtcgcg gctggacaac
     1321 gtcgtgtacc gggccggttt cgcgatgaac cgcccgatgg cgcggcagct cgtctcgcac
     1381 gggcacttcg aggtcaacgg ccgcaaggtc aacatcccgt cgttccgggt caagccgggc
     1441 gacgtcgtga cggtgaggaa ccgctccaag gcgatgggac ggatcgtcga gaacacgtcc
     1501 tacgccgagg gtcgggagat ccccgactgg ctcggatcga acctccggga gatgcgcgtc
     1561 gaggtgcgcg cgctgccgga gcggggccag atggacatcc ccgtccaaga gcagctcatc
     1621 gtcgagtact actccaagta acccacgcag cacggcaacg cggagaggag ccccatggat
     1681 ctgctgatcg ttcaacgtcc acagatcacc gaagaggtga tctcggacgt acgcaacaag
     1741 ttcaccatcg agccgctcga gcccgggttc ggatacacgc tcggcaactc gctgcgccgc
     1801 acgctcctct cgtcgatccc cggagccgcc gtcacgcagg tcaagatcga cggcgtgctg
     1861 cacgagttct cgacgatcga cggagtctcc gaggacgtga cgaacatcgt cctgaacctc
     1921 aaggagctcg tgatccgcag cgagaacgag gaggcgtcga cggtccacct gcgggtgacc
     1981 ggtcccgcca ccgtgaccgc gggtgacatc gagctcccgg ccggggtcga gatcctcaac
     2041 cccgacaagg tcatcgccac gctgaacaag aacgcgacgc tctcgatgga gctgcgcgtc
     2101 cagcagggcc gcggctacgt cccggctgcg tcggccgcga aggaggcgat cgggacgatc
     2161 ccgagcgacg ccctctactc accggtgaag cgtgtgacgt acgaagtcga gccgacccgc
     2221 gtcgagcaga tgacgaacta cgaccgtctg atcctcgacg tcgagaccga cggctcgatc
     2281 acgccgagca acgcgctcag cgcggccggc tcgacgctcg tcaacctgtt ccagctgttc
     2341 gccgggatgg gcgagggccc cggcctcgac ctcggccccg agccgggcga ggaagaggag
     2401 tccggcgtcc tggcgcagcc gatcgaggac atggacctca ccgtccgctc gtacaactgc
     2461 ctgaagcgcg agggcgtcac gacggtcggc gagctcggcc acaagaccga ggacgacctg
     2521 ctcgagctcc gcaacttcgg tcagaagtcg atcgacgagg tcaaggccaa gctcgaggag
     2581 atgggcctgg gcctccggaa gaaggagttc tagacgatgc cggcgccgaa gaagggtccg
     2641 aggctggggt cgaacccctc gcatcaggcg ctgatgctgt ccaacctcgc gctctcgctg
     2701 ttcgagcacg agcgcgtgaa gacgaccgag gcgaaggcca agctgctccg tccgtacgcc
     2761 gagcgtctga tcaccaaggc caagaagggc gacgtgcaca accgccggca ggtcctctcg
     2821 acgatcgagg accgcgcggt cgtgcacaag ctcttcgccg acatcgcgcc gcgcttcgcc
     2881 gaccgcaacg ggggctacac gcggatcctc aagctcggcc cccgcaacgg cgacggcgcg
     2941 ccgatggcac tgatcgagct cgtcgagagc gcgacggtga cgacggtcga gtccgagccg
     3001 gacgagggcg ggaagcgccg ccgtctcccg cggccccgcc gccggggcgc gtcggccgac
     3061 ctcccgcagg acaagcctgt tcgctcgcgc gcggcggacg ctgccgcggc cggtgcggct
     3121 gctgccgtcg gtggtgacga cgaggacgag gggaccgagg acaccggcgc cgacgaggct
     3181 gctgccgagg aagcgacggc agaggccgaa gaggccggtg ctcccgacga cgacgctccc
     3241 agcgcgggcg gcgccgccac ggaaaacgac gactcctcca actgaacctc cggctggacc
     3301 tcgcgtacga cggcgcgccg ttccgcgggt tcgcacggca gccggggcag aagaccgtcc
     3361 agggtgatct cgaagacgcg ctgacgcgcg tgctgcgcgc tacgccggtc ctgacggtcg
     3421 cgggacgtac ggacgccggc gtccacgcgg tcggccaggt cgtgtccgcc gacggcgtgc
     3481 ccgacgacgc ggaccccgag gggctccggg gctcgctcaa ctcgatctgc gggccctcga
     3541 tcgtcgtcca cggctgcgta cgcgctccgg acgagttcaa cgcgcgcttc gacgcccgtt
     3601 cgcgcaccta cgtctacgcg atctccacgg aagacgtccc cgacccctgg ctggggcgca
     3661 ctgcatggca ccaccccggc cccctcgacc tcgacgcgat gtcggaggcc gcagggcacc
     3721 tcctcggccg gcgggacttc tcgtcgttcg gccgtctccc cgcccccgac gcgagcccgg
     3781 agcggaccct ctacgagctg acgtgctcgg ccgccggctc gatcgtcagg atccgcgccc
     3841 gagcggacgc cttcatccag cagatggtcc gttctctcgt cgggacgctc gtcgcagttg
     3901 gcgaggggaa gatggagccg gatcggatgc cgtccgtcct cgcggcgaag gatcgtgccg
     3961 cggcggggcc ggtcgctcct ccccacggcc tctgtctggt ctcggtcgaa tacgagaccg
     4021 gttggagccg gccgtttgac cccttcgaat agcgcctata tcctgcactt tccccttttt
     4081 gggcacccgc ttgcgccagc gcgggacgag caaggattgt gatgaagacc cacgtaacca
     4141 agccgaacga gataacgcgc gagtggtgga tcgtcgacgc cgacggtgtc gtactcggcc
     4201 gtctcgcatc cgaggtcgcg aagcggctgc gcggcaagca caagccgtcg tacagccccc
     4261 acctcgacac cggcgaccac gtgatcatcg tgaacgcgtc gaaggtcgtg ctcaccggca
     4321 gcaagctgac cgacaagcag taccaccgcc actccggcta ccccggcggt ctccgttcgg
     4381 tcccttactc caagctcatg gccgagcgcc ccgacatcgc cgtcgagaag gccgtcaagg
     4441 gcatgctgcc gagcaacagg ctcggccgcg cgatgctgaa gaagctcaag gtctacaacg
     4501 gcgcggagca cccgcattcg gcgcaggccc ccaagccgct cgacgtcaag gacgtcgcgg
     4561 caccgcacaa cccggagcag gcgcacactg tcgaggaggt aaccggtggc tgaggcactc
     4621 gtccgcgcta ccggccggcg taaggaagcg gtgtgccgtg cacgtcttct tccgggctcc
     4681 ggccgctggg agatcaacgg ccgtccgatc gaggactact tcccgagcgc gacccaccgc
     4741 atgatcatca ccgagcccct tcgtctcacc gagaccgagg gccgctacga catcatcgcc
     4801 aagatcgagg gcggcggcgt cagcggacag gccggtgcgc tcaggcattc gatctcgaag
     4861 gcgctggtcg aactcgaccc cgacctgcgg ccggttctca agaaggccgg cttcctgacc
     4921 cgcgacgcac gcgagaaaga gcgtcgcaag tacgggctca agaaggcccg caaggcgcct
     4981 cagtactcga agcgctaagc ctcctccgca tcgctccatg acgtcccccg cgcctcagcg
     5041 cgatctcgtg ccgcggcgcg aggcgtttct gtggtggctg aagctcggct tcatcaactt
     5101 cggcgggccc gccgggcaga tcgcgatcat gcacgacgag ctcgtcgacc ggcggcgctg
     5161 gatcaacgac gaccgcttcc tccacgcgct gaacttctgc atgctgctgc cgggccccga
     5221 ggcgcagcag ctcgcgatct acgtcgggtg gctgctcaac gggacgctcg gcgggatcgt
     5281 cgccggcgtc ctgttcgtgc tcccggcgtt ctttctgatc ctcggcctct cgtggacgta
     5341 cgccgcgcac ggggacgtca cgtggatcgc ggccgtcttc tacgggctgt gctcggcggt
     5401 gatcgggctc gtggcggtcg cggtgatccg ggtgggctcg aaggcgctga agacgccggc
     5461 ggcgtgggcc gtcgccgcgc ttgcgttcgt gacgatcttc tttctcgacg tgccgttccc
     5521 gttgatcgtg ctcggggctg cgatcgcggg ctacgtggga tcgaggatcg acccgcgctg
     5581 gtggccggcc tcgggccacg gagaagaaag agaaggcgat cacgtcatct cgggggcggc
     5641 actcgaacgg gtgcgcccga cgtggcgccg cagcgtcgcc gtgctgctga ccgggctcgc
     5701 gatctggtgg gggccgttgc tgctcgtgct cttgtggcgc gggaccgagg acacgctttc
     5761 caaggaggcg ttgttcttca gccaggcggc gatggtgacg ttcggcggcg cgtacgcggt
     5821 gctctcgtac atcaacgagg cggccgtgca gcagttcgga tggctggcgc ccgggcagat
     5881 ggtgaccggc ctcggtctgg ccgagtcgac acccggcccc ctgatcatgg tgaccgagtt
     5941 cgtcggcttc ctcggaggct acaacttccc gggcgggctc gatcccgtcg tcgcggggat
     6001 cctcggggca acgatcacga cctgggcgac gttcgcgccg tgcttcttgt ggatcttcct
     6061 gggcgcgccg tacgtcgagc ggctgcgagg gaagcggggg ctgagcgcgg cgctgtcggc
     6121 gataacggcc gcggtggtag gggtgatcct gaacctcgcc gtgacgttcg gggccgtagc
     6181 gttgttcgac gacgtcgacg gctcgcgggg cttcccggtc ccggacccga cgtcgataga
     6241 cgcgttcgcg gccgcgctgg caatcgcgtc gttggtggcg ctgtggcgct acaaggtcaa
     6301 cgtgctgtgg gtggtgctcg ccagcgcggg tgccgggctg ctctatcgtc tcgtcgtctg
     6361 aagggggatc ccgtgaagtg gttcggcgtc aggtgcgtgt tctggcacga cggtgactcg
     6421 ttcgaggagc gcatcaccct gtggcgcgcg gagtcgatcg acgaagccat agcgcaggcc
     6481 gaggaagagg cgcgcgcgta ctgcgaaggc atcgactgca agtacgtcgg tctcgcccag
     6541 ggctacggcc cggcggccgg cccgggaggc gacgacgccg tcgagatcgt ccccggaacg
     6601 gaggtcttct cgttgttccg cctcagcgag ttcgagccgc gcgagtacct cgaccacttc
     6661 ttcgacacgg gatcggaggt ccaccaagac tggaccccgg gctcgttccc ctcctagccg
     6721 gcgagcatcc tcctgagcac catctgcagc acgccgccgt ggcggtagta gtccagctcg
     6781 accgccgagt cgatccggca ctcgacctcg aactctttct cgccggcccg gacggtcagc
     6841 gtcgcgtgcg gcttcagccc gtccgcgatc cccgttatct cgaacgtctc gtgacccgtc
     6901 aggcccagcg actcggcggt gtcaccttcg cggaactgca gcggcaggat gcccatgccc
     6961 acgaggttgc tgcggtggat gcgctcgaag ctctcggcga tcgcggccct cacgcccagc
     7021 agcgccggtc ccttcgcggc ccagtcgcgc gagctccccg acccgtactc cttgccggcg
     7081 atgatcagta gcggcgtccc ctcggaccgg tacgcctcgg acgcctcgaa gatcgtcgtg
     7141 acctcgccct ccgggagctt cttcgtccag ccgccttcct tgccctcggc gagcacgttg
     7201 cggaagcgga tgttcgcgaa cgtcccgcgc atcatgacct cgtggttccc gcggcgcgag
     7261 ccgtagctgt tgaagtcgcg cacctcgacg ccctgctcct tcaggtactt ccctgccgga
     7321 gacgccggcg cgatcgatcc ggccggcgag atgtggtccg tcgtgacgga atcgcctacg
     7381 aggcagagca ccctcgcgcc ggtgatgtcg acggggtccg agggctcggg gccgaggtcc
     7441 tcgaagaacg gaggctcccg cacgtacgtc gactcgccgt cccatgcgta cagggagccg
     7501 gtcggtgagg ggagctcctg ccagttctcg tcgccgtcga agaccttcgc gtactcctgc
     7561 gtgaagagct cggccgtgac ggcgttcatc gccccggcca cctcttcggc tgtgggccac
     7621 agatccttca ggtacacggg ctgtccgtcc gcgccggtgc cgagcggctc ggtagtcatg
     7681 tcgatgtcgg cccggcccgc gagcgcgaac gcgacgacga gcggtggcga cgcgaggtag
     7741 ctcgccttca cctgcgggtg gatgcggccc tcgaagttgc ggttgccgga cagcaccgac
     7801 acgaccgcga ggtcgtcggc gtcgatcgcc gctgcgacct cgtcgggtac ggctgcacga
     7861 cctgcatcgg caactccggg ccgctgatcc ccgaggtgag caaggccgtc aacgacgccg
     7921 acctcgcggt gaccgccgtg ctgtcgggca accgcaactt cgagggccgg atcaacccgg
     7981 acgt
//
LOCUS       DASQDO010000037         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_801174, whole genome shotgun sequence.
ACCESSION   DASQDO010000037 DASQDO010000000
VERSION     DASQDO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801174"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VEV43_01180"
     CDS             <1..669
                     /locus_tag="VEV43_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYP22160.1"
                     /translation="RRLWAPPDTDPRRPLPLLVAHDGPEYAKFSGLTHFLSAMVAQGR
                     LPRMRAGLVGPVDRDNTYSASAAYSRAMAHEILPALDRLAPTPHGRRARVGMGASLGG
                     LAMLVQHRKAPATFGGLYLQSGSFFRQRFDRHESGFSRFRRISRFVGGVLAVDDWAHP
                     VPVTMTCGTVEENLANNRAVRDALEAQGYEVYLFENRDAHNWVGWRDTFDPHLTDLLA
                     KVWG"
     gene            666..1406
                     /locus_tag="VEV43_01185"
     CDS             666..1406
                     /locus_tag="VEV43_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYP22161.1"
                     /translation="MRRDHISLPAPAIGGHGDVIAYGHWGRPLLVFPSQQGRSWDYEN
                     NGMIGSLAHLIEGGRLKVYCVDSYDAGSWDAHDLSLEERAQRHGGYEDWIVNQVVPFI
                     HMDCGGYVETMTTGCSFGAYHAANFALKRADLFPLAICLSGVYDVSVVGHGHRGDAFY
                     FNNPMDYVAHMGGDHLEWLRDRVSLLLVCGQGQWEDTTNALQSTKQFAGVLASKGIPH
                     ELDLWGHDVPHDWPSWRNQIAHHVPRFC"
     gene            1420..>2411
                     /locus_tag="VEV43_01190"
     CDS             1420..>2411
                     /locus_tag="VEV43_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22162.1"
                     /translation="MNTHLIGLLLGTEEDWPGAFEGIMRRLQPKIDFKGETHTFETER
                     VTIEPFDLRAVPRHSLVIDRLAHWYYVPREWLKKVALMNDVYLMNNPFTFQSMEKHAA
                     YCAMIRLGLKVPETWMIPTKKPPENPRFPYTAERYNQPFDLSDVADRVGYPLFMKPFD
                     GGAWVGVTRIADDIELHHWYDQSGERLMHLQQAIEDFDVFSRSLSIGAETMVMRFEPS
                     RPMHDRYQVSHGFLTPEVGREVVNIGRLVNSFFRWEFNSCETLVKGTEVYPIDYANAC
                     PDISIISLHYYFPWAIKALVKWALYCTVTDRRMRVDLDPRPFFEIADRTDLSYE"
BASE COUNT          402 a          806 c          822 g          381 t
ORIGIN      
        1 cggcggctgt gggcgccgcc cgacacggac ccgcgccggc cgctgccgct gctcgtcgcg
       61 cacgacggcc cggagtacgc caagttctcg gggctcacgc acttcctgtc ggcgatggtg
      121 gcgcagggac ggctcccgcg gatgcgagcc gggctcgtcg gtcccgtcga ccgcgacaac
      181 acctattcgg cgtcggctgc gtactcgcgg gcgatggcgc acgagatcct gccggcgctg
      241 gaccgcctcg ccccgacgcc acacgggcgc cgggcgcgcg tcgggatggg cgcgagcctc
      301 gggggcctcg cgatgctcgt gcagcatcgc aaggcgcccg ctacgttcgg cgggctgtac
      361 ctgcaatccg gcagcttctt ccggcagcgc ttcgaccgtc acgagtcggg gttctcccgc
      421 ttcaggcgga tctcccgctt cgtcggcggg gtgctggccg tggacgactg ggcacacccg
      481 gtgccggtga cgatgacgtg cgggacggtg gaggagaacc tggcgaacaa cagagcagta
      541 cgcgacgcgc tcgaggcgca aggctacgag gtctacctgt tcgagaaccg tgacgcgcac
      601 aactgggtgg gctggcgcga cacgttcgac ccgcacctga cggacctgct cgcgaaggtg
      661 tggggatgag gcgcgaccac atctcgctgc ccgcccccgc gatcggcggc cacggtgacg
      721 tcatcgcgta cggccactgg gggcggccgc tgctggtctt cccgtcgcag cagggccgct
      781 cgtgggacta cgagaacaac gggatgatcg gctcgctcgc gcacctgatc gaaggcgggc
      841 gcctgaaggt ctactgcgtc gacagctacg acgcggggag ctgggacgcg cacgatctct
      901 cgctcgagga gagggcccag cgtcacggcg gctacgagga ctggatcgtc aaccaggtcg
      961 tgcccttcat ccacatggac tgcggcggat acgtcgagac gatgacgacc ggctgcagct
     1021 tcggcgccta tcacgcagcg aacttcgcgc tgaagcgcgc cgacctgttc ccgcttgcga
     1081 tctgcctgag cggcgtctac gacgtgtcgg tcgtcgggca cgggcatcgc ggcgacgcgt
     1141 tctacttcaa caacccgatg gactacgtcg cgcacatggg cggcgaccac ctcgagtggc
     1201 tgcgcgaccg ggtgagcctg ctgctcgtct gcggccaggg gcagtgggag gacacgacga
     1261 acgcgctcca gagcacgaag cagttcgccg gcgtgctcgc gagcaagggg atacctcacg
     1321 agctcgacct gtggggtcac gacgtcccgc acgactggcc gtcgtggagg aaccagatag
     1381 cccatcacgt accgaggttc tgctgacggg gaggcgaatg tgaatacgca cctgatcggg
     1441 ctgttgctcg gcaccgaaga ggactggccc ggcgcgttcg agggcatcat gcggcggctg
     1501 cagccgaaga tcgacttcaa gggcgagacg cacacgttcg agaccgagcg cgtcacgatc
     1561 gagccgttcg acctgcgcgc ggtgccgcga cattcgctcg tcatcgaccg cctcgcgcac
     1621 tggtactacg tgccgcgcga gtggctgaag aaggtcgcgc tgatgaacga cgtgtacctg
     1681 atgaacaacc cgttcacgtt ccagtccatg gagaagcacg cggcctactg cgccatgatc
     1741 cggctgggtc tcaaggtgcc cgagacgtgg atgatcccga ctaagaagcc gccggagaac
     1801 ccccggttcc cgtacacggc cgagcgctac aaccagccgt tcgacctctc cgacgtagcg
     1861 gaccgcgtcg ggtaccccct gttcatgaag ccgttcgacg ggggggcctg ggtcggcgtc
     1921 acgaggatcg ccgacgacat cgagctccac cactggtacg accagtcggg cgagcggctc
     1981 atgcacctcc agcaggcgat cgaggacttc gacgtgttct cgcggtcgct gtcgatcggg
     2041 gccgagacca tggtcatgcg gttcgagccg tcccggccga tgcacgaccg ttaccaggtg
     2101 tcgcacggct tcctcacgcc ggaggtaggc cgcgaggtcg tgaacatcgg ccggctcgtc
     2161 aactcgttct tccggtggga gttcaactcc tgcgagaccc tcgtgaaggg gaccgaggtc
     2221 tacccgatcg actacgctaa cgcgtgcccc gacatctcga tcatcagcct ccactactac
     2281 ttcccgtggg cgatcaaggc gctcgtgaag tgggcgctct actgcacggt cacggaccgg
     2341 cggatgcgcg tcgacctcga cccccggccg ttcttcgaga tagccgaccg caccgacctg
     2401 agctacgagg a
//
LOCUS       DASQDO010000038         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_617761, whole genome shotgun sequence.
ACCESSION   DASQDO010000038 DASQDO010000000
VERSION     DASQDO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617761"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..784
                     /locus_tag="VEV43_01195"
     CDS             131..784
                     /locus_tag="VEV43_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22163.1"
                     /translation="MITALTGTPLDQVVEHAESAREALVGAGLVALLVAFVLAFLDDH
                     DRGTVRSSLESATTAFKNAAQRPDNGSDGPAMAGAVEFVRAVAGPTETLFRRSRAVVA
                     LVIGGVLLIASALVPLFETALVANHCAGKVANAEATPEPSPSDAGTSTPDTGDQDPGG
                     APAGDEEADQTPKENEVGNGGGKGSDRGETSAAVAESSVPKVFYPPACRDPERDPLG"
     gene            complement(791..1153)
                     /locus_tag="VEV43_01200"
     CDS             complement(791..1153)
                     /locus_tag="VEV43_01200"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HYP22164.1"
                     /translation="MKSYTIEEANALLPYLAPTLVELREKFELAESARENVAHAAAGN
                     GWSEKREQWSVTMARVAELMERLQEWELELRDIATGLVDFPAVLHDEEVYLCWRLGEP
                     EVGYYHSRTEGFGGRRRL"
     gene            complement(1150..1842)
                     /locus_tag="VEV43_01205"
     CDS             complement(1150..1842)
                     /locus_tag="VEV43_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYP22165.1"
                     /translation="MRIAVVSDIHGNLLALDAVMTDIEKHSPDEVWCGGDIGWGGPWA
                     SECIARVRDAGWTTIKGNTDIWITGDPQGLDSEEDRAELQGVAAAHNISKEDAEWLIN
                     RPLGHQGPGSILLVHGTPRSPFVAPLPWDPASAFEDYEGQATVVVYGHVHHSFVRRLS
                     DGTIVANAGSVGLPMDGLTACYMLIDQQGAEWCIRHRRVPFDRRAGIAQARQMGGPVG
                     ERFLRYMEMSES"
     gene            1887..>2696
                     /locus_tag="VEV43_01210"
     CDS             1887..>2696
                     /locus_tag="VEV43_01210"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HYP22166.1"
                     /translation="MATLADRIQAVSGLDAEEAQHLRALCSSWQVLADLSFSDLLLYV
                     SVGGRSDEFHVAAQLRPFTSQTLYPHDMVGVRVTQPEQPIVERAFREGRIWQQDDPVL
                     VDGIPIRMDAVPVRFRGDVVAVVTKEGSPATSRRPGRLEQVYLGAADQVSRMIAAGDF
                     PWPDTPAGEWPRVGDGLFVLDEIGRVSWASPNALSSLRRLGVKQNALGRWLDELGLGE
                     TPVHSALESRRLKDGELVRGDTHVSIRVLPLTDGDSTVGALALAKDVTEIRQ"
BASE COUNT          408 a          941 c          939 g          408 t
ORIGIN      
        1 gaagcgagca tctgcatcga cgaaagggag aagaggcgac acgcgatcgt gaagatcctg
       61 acgacgttcc gcgagtcgca gccccaggac ggcgagggcg agtgaagccc gccgagaagg
      121 tcgtcgcgag atgatcaccg ccttgacggg cacgccgctg gatcaggtcg tcgagcacgc
      181 agaatcggcg agagaagcgc tggtcggggc cgggctggta gcgctgctcg tggccttcgt
      241 cctcgcgttc ttggacgacc acgaccgggg aacggtgagg tcgtcgctag agagtgcgac
      301 gacggcgttc aagaacgcgg cgcagcgacc tgacaatggg tcggacggcc ctgcgatggc
      361 aggcgcggtc gagttcgttc gcgccgtcgc cggccccaca gagaccttgt tccggcgatc
      421 ccgcgcggtg gttgctctcg tgatcggcgg cgtcctgctg atcgcgagcg cgctcgtccc
      481 gctgttcgag acggccctcg tcgcgaacca ctgcgcgggc aaggtcgcca acgccgaagc
      541 gacgcccgag cccagccctt cggatgcggg aacgtcgacc ccggataccg gcgaccagga
      601 ccccggtggg gcacccgcag gagacgagga ggccgatcag accccgaagg agaacgaggt
      661 cggcaacggc ggcggtaagg ggagcgaccg aggggagaca tcggccgcgg tcgccgagtc
      721 atccgttccc aaggtgttct atccaccggc gtgccgcgac cccgagcgag acccgctcgg
      781 gtagcgccct ctagagtctt cgccggcccc cgaagccctc cgtccgcgag tggtagtacc
      841 cgacctccgg ctcgcccagg cgccagcaca ggtacacctc ctcgtcgtgc agcacggccg
      901 ggaagtcgac gagccccgtc gcgatgtccc gcagctcgag ctcccactcc tgcagccgct
      961 ccatcagctc cgccacccgc gccatcgtca ccgaccactg ctcgcgcttc tcggaccagc
     1021 cgttgccggc ggccgcgtgc gcgacgttct cgcgcgcgct ctcggcgagc tcgaacttct
     1081 cccgcagctc gactagcgtc ggcgcgaggt acgggaggag cgcgttcgcc tcctcgatcg
     1141 tgtacgactt caactctcgg acatctccat gtacctgagg aaacgctcgc ccaccggccc
     1201 ccccatctgt ctcgcctgcg cgatcccggc ccgccggtcg aacggcacgc ggcggtggcg
     1261 gatgcaccac tccgccccct gctggtcgat cagcatgtag cacgcggtca ggccgtccat
     1321 cgggagaccc accgatcctg cgttcgcgac gatcgtcccg tcggacagtc tccgcacgaa
     1381 gctgtggtgg acgtggccgt agacgaccac cgtcgcctgg ccctcgtagt cctcgaacgc
     1441 cgacgcgggg tcccacggga gcggcgcgac aaacggggaa cgcggcgtgc cgtggacgag
     1501 caggatcgag cccgggcctt ggtggccgag cggccggttg atgagccact cggcgtcttc
     1561 cttcgagatg ttgtgcgcgg ccgcgacgcc ttggagctcg gcgcggtcct cttccgagtc
     1621 gagcccctgc gggtcgcccg tgatccagat gtcggtgttg cccttgatcg tcgtccagcc
     1681 cgcgtcgcgc acgcgtgcga tgcattcgga cgcccacggg ccgccccacc cgatgtcacc
     1741 gccgcaccac acctcgtcgg gcgagtgctt ctcgatgtcg gtcatgacgg catcgagcgc
     1801 gagcaggttg ccgtgtatgt cggaaacgac cgcgattctc actgtgagcc ccctccgatc
     1861 ggtcggcggc atcctacgat tccggggtgg cgacccttgc agaccgcatc caagccgtct
     1921 ccggcctcga cgccgaagag gctcagcacc tgcgcgcgct ctgctcgtcg tggcaggttc
     1981 tcgccgacct gtcgttctcg gacctcctgt tgtacgtaag cgtcgggggg cgctccgacg
     2041 agttccacgt cgccgcgcag ctgaggccgt tcacgtcgca gacgctctac ccgcacgaca
     2101 tggtcggggt gcgcgtgacg caaccggagc agccgatcgt cgagcgcgcg tttcgcgagg
     2161 gtcgcatctg gcagcaggac gatcccgtgc tcgtagacgg aatcccgatc cggatggacg
     2221 cggtcccggt gaggttccgc ggcgacgtcg tagccgtcgt gaccaaggag gggagcccgg
     2281 cgacgtcgcg gcggccgggg cggctggagc aggtctacct cggggcggcc gaccaggtct
     2341 cacggatgat cgcggccggg gacttcccgt ggccggacac gccggcgggg gagtggccgc
     2401 gcgtcggcga cgggctgttc gtgctcgacg agatcggccg cgtgagctgg gcgtccccga
     2461 acgcgctgtc gtcgttacgg cggctcggcg tgaagcagaa cgcgctcggc cggtggctcg
     2521 acgagctggg cctcggcgag acccccgtgc acagcgcgct cgagtcgcgt cggctgaagg
     2581 acggcgagct cgtgcgcggc gacacgcacg tctcgatcag ggtgctcccg ctgacggacg
     2641 gcgactcgac cgtcggagcg ctcgcgctcg cgaaggacgt caccgagatc aggcag
//
LOCUS       DASQDO010000039         5040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_617785, whole genome shotgun sequence.
ACCESSION   DASQDO010000039 DASQDO010000000
VERSION     DASQDO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617785"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VEV43_01215"
     CDS             <1..255
                     /locus_tag="VEV43_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HYP22167.1"
                     /translation="IADIGLMGCLISRPDGTKADGFQVHVGGHLGEDHKLGRKVRGLK
                     VPAGDLADYVERVIRRYSSSAHDGEPFHRWAERAPEAWLK"
     gene            252..401
                     /locus_tag="VEV43_01220"
     CDS             252..401
                     /locus_tag="VEV43_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22168.1"
                     /translation="MSGSQPFYCPYCGEQDFEPASEEAGTFVCGSCDRSWKVQLLGVN
                     VSGRS"
     gene            402..1760
                     /gene="cobA"
                     /locus_tag="VEV43_01225"
     CDS             402..1760
                     /gene="cobA"
                     /locus_tag="VEV43_01225"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYP22169.1"
                     /translation="MAFGYPVSLDVTGRRCVVIGSGPLAKEKVDGLEEAGAEVVLLSS
                     YSVGALRGAFLAVVTDPDPLLHAAVAEEARAERVLLNCMDDVPYCDFAAPSIVRRGDL
                     RISISTGGKAPALAKRLRLKLTEEIDEAYGELVDLLGEVRAETIATRGDFETWRRRWQ
                     HALDQPLLELVREGRVDEAKELVRRCLTQDPGPRPARRPEPPRGRRWVNGGVSIVGAG
                     PGDPGLITVRGSELLAACDVVVYDRLVHPELVEGKRAIYVGKQAGRHCVTQEQINDLL
                     VRLARSGEHVVRLKGGDPFVFGRGAEEARSLAAAGVPFEVVPAPSSAVAAPAYAGIPV
                     TDRGVATSVAFVTGSAGAGREPNWRGLAGSADTLVILMPGRDLSRVVEELVAGGLCPE
                     TPAAVVANGTLEDQRVARCALRELPGREPASPSLVVVGEVADESRGLAWFREVAAAAT
                     TG"
     gene            complement(1750..3024)
                     /locus_tag="VEV43_01230"
     CDS             complement(1750..3024)
                     /locus_tag="VEV43_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22170.1"
                     /translation="MPNLRARLLGAATVLFVLLPGLALADDWTWRDRDDLDRRLSIHS
                     VTAGHDEEGPTVTVTFDRPLRAEDMGEKDFVIVAIESDGEEPNEEWIYLVSIDGQLQR
                     FSYNPHSEETYQIADYTWSQPIPESVTFKVTAFSPGHSHAFFAGSYTETAPGCAEGCW
                     DTAPNRGGLIHDWTPPWVHAVREPSTWTYQRTLKFYWEASDYGLSGFRSSTLLMSTPG
                     SGKWEPIARRTKPGRYVVFVRPLTQGSNVMLRPVAVDGAKNKTLGPIRRTRVPYDQTN
                     PKGGRTFTGKWAEEATTSFGGTVHTSTAPMDTFSYKARGTLYCFHGWWREGVRATFEV
                     GGETAEIDTLAGGSASGFPTCIETETAERRTATLTVHEGRVSVDWYWAGIYPPATDTA
                     EDEPSAREGHPVPTSTAPSARAMVNARRSLSR"
     gene            complement(3212..3664)
                     /gene="nusB"
                     /locus_tag="VEV43_01235"
     CDS             complement(3212..3664)
                     /gene="nusB"
                     /locus_tag="VEV43_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYP22171.1"
                     /translation="MKARRTARRLALDVLYEAEIRDELPSEALEARRSGGWVVATASD
                     SEEPPDEPTEEALAYAADLVAGVQEHHADIDVLIAAHADRWAIERMPVVDRTLLRIAL
                     FELLWREDIPVAVGINEAVELAKSYSTEDSGRFINGLLGRIVEVEKGA"
     gene            complement(3678..4241)
                     /gene="efp"
                     /locus_tag="VEV43_01240"
     CDS             complement(3678..4241)
                     /gene="efp"
                     /locus_tag="VEV43_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HYP22172.1"
                     /translation="MISTNDMRPGQTLNLDNGLFTIVDYQHVKPGKGQAFVKTKLKNL
                     KNGAVTEKTFRADEKVDLAVLDKREMQYLYEDDAGLVFMDNESYEQVHVDPALLGDSR
                     KFLKDSTICMVPVYEGTPVGAEMPVSMELEVVDTEPGHKGDRVSGALKPATVETGAVV
                     QVPLFVEKGERIKVDTRTGEYQSRVQG"
     gene            complement(4257..>5040)
                     /locus_tag="VEV43_01245"
     CDS             complement(4257..>5040)
                     /locus_tag="VEV43_01245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYP22173.1"
                     /translation="TMTVATKAQLEGKLEVELAPTKNLVENLRRTKDAEEVSLLREAV
                     RIADEAFGWIVERLGPGRTEREVALDLDVEMRRRGADSISFDPIVGTGPLSAHIHHTP
                     SDREMQNGDLVLLDFGARWQGYCSDLTRTVALGSASDHHREVYGFVLASQTAGIEATR
                     AGAACSGVDAAARAVIDDAGYGATFGHGLGHGVGLDIHEAPRFHKTSEDTLVAGDVMT
                     IEPGIYETGSGGIRIEDCVLVTDDGCEVLGKAPKDTLIEISE"
BASE COUNT          663 a         1724 c         1797 g          856 t
ORIGIN      
        1 atcgccgaca tcgggctgat gggctgtctg atctcccggc ccgacggcac gaaggcggac
       61 gggttccagg tgcacgtcgg gggtcacctc ggcgaggacc acaagctcgg gcggaaggtg
      121 cggggcttga aggtcccggc aggtgacctc gcggactacg tcgagcgagt gatccggcgg
      181 tactcgtcgt ccgcgcacga cggcgagccc ttccatcggt gggcggagcg cgcgccggag
      241 gcctggctca agtgagcggg tcgcagccct tctactgccc gtactgcggg gagcaggact
      301 tcgagccggc gagcgaggag gccgggacgt tcgtgtgcgg gtcttgcgac cggtcgtgga
      361 aggtgcagct gcttggagtc aacgtgagcg ggcgctcgta gatggcgttc gggtatccgg
      421 tctcgctcga cgtgacgggc cgccgctgtg tcgtgatcgg ctccggtccg ctggcgaagg
      481 agaaggtgga cgggctcgag gaggcggggg ccgaggtcgt gctcctgtct tcgtactcgg
      541 tcggtgcact gcggggcgcg ttcctcgccg tcgtcaccga tcccgacccg ttgctgcacg
      601 ccgccgttgc cgaggaggct cgggccgagc gcgtgctgct caactgcatg gacgacgtcc
      661 cctattgcga cttcgccgcg ccctcgatcg tgcgccgcgg tgacctgcgg atctcgatat
      721 cgaccggggg caaggcgccc gcgctcgcga agcggctgcg cctcaagctc accgaggaga
      781 tcgacgaggc gtacggcgag ctcgtcgacc tgttgggcga ggtgcgggcc gagaccattg
      841 ctacccgcgg tgacttcgag acctggcgcc ggcgctggca gcacgctctc gaccagccgc
      901 tgttggagct cgtgcgcgag ggccgcgtcg acgaggcgaa ggagctcgtg cggcgttgtc
      961 tcacgcagga cccggggccg cggcccgcgc gtcgtccgga gccgccgcgc gggcgtcgct
     1021 gggtgaacgg tggcgtctcg atcgtggggg ccggaccggg cgacccgggc ctgatcaccg
     1081 tgcgcggctc ggagctgctc gcggcgtgcg acgtcgtcgt ctacgaccgg ctcgtgcatc
     1141 ccgagctcgt cgagggaaag cgcgcgatct acgtcggcaa gcaggcggga cgtcactgcg
     1201 tcacgcagga acagatcaac gacttgctcg tgcggctcgc gcggtccggc gagcacgtcg
     1261 tccgtctcaa gggcggcgac ccgttcgtgt tcggcagggg ggccgaggaa gcgcgctctc
     1321 tggcggctgc cggcgtgccg ttcgaggtcg tgcctgcgcc gtcgtccgcc gtcgcggccc
     1381 ccgcgtacgc gggcatcccg gttaccgacc gcggcgtcgc gacgagcgtc gccttcgtga
     1441 cgggctcggc gggcgcgggg cgggagccga actggcgggg gctcgccggg tcggccgaca
     1501 cgctcgtgat cctgatgccg ggccgcgatc tctcgagggt ggtcgaggag ctggtcgccg
     1561 gagggctctg ccccgagacg cctgcagccg tcgtcgcgaa cgggaccttg gaggatcagc
     1621 gggtcgcgcg atgcgcgctg cgcgagctgc ccggtcggga gccggcgtcg ccttcgctcg
     1681 tggtcgtcgg cgaggtcgcg gacgagagcc gggggctcgc gtggttccgc gaggtcgccg
     1741 cggccgccac taccggctga gggacctccg cgcgttcacc atcgcgcgcg ccgacggcgc
     1801 cgtcgacgta gggacggggt ggccttcgcg tgcggacggc tcgtcctcgg ccgtgtccgt
     1861 agccggcggg tagatgccgg cccagtacca gtcgacgctc acgcggccct cgtggacggt
     1921 gagcgtcgcg gtgcggcgct ccgccgtctc ggtctcgatg cacgtcggga agccgctggc
     1981 cgagcctcct gcgagagtgt ctatctccgc ggtctcgccg ccgacctcga acgtcgcgcg
     2041 cacgccctcg cgccaccagc cgtggaagca gtagagcgtc cctcgcgcct tgtacgagaa
     2101 cgtgtccatc ggcgccgtcg aggtgtgcac ggtcccgccg aacgaggtcg tcgcctcctc
     2161 ggcccacttc ccggtgaacg tccggccccc cttggggttc gtctggtcgt acggcacccg
     2221 cgtccgtcgg atggggccga gcgtcttgtt cttcgcgccg tcgaccgcca ccggccgcag
     2281 catgacgttc gagccctgcg tcaacggacg cacgaagacc acgtagcggc cgggcttggt
     2341 gcgtctggcg atcggctccc acttgcccga gccgggagtc gacatcagaa gggtcgagct
     2401 gcggaagccg gacaggccgt agtccgacgc ttcccagtag aacttgagcg tccgctggta
     2461 cgtccacgtg gacggctccc tcacggcgtg gacccacggg ggcgtccagt cgtggatcaa
     2521 gccgccgcgg ttgggggccg tatcccagca gccttcggcg catcccggcg cggtctcggt
     2581 gtacgagccg gcgaagaacg cgtggctgtg ccccggcgag aaggccgtga ccttgaacgt
     2641 gaccgactct gggatcggct ggctccacgt gtagtcggcg atctggtacg tctcttcgga
     2701 gtgcgggttg taggagaagc gctggagctg cccgtcgatc gagacgaggt agatccactc
     2761 ctcgttcggc tcctcgccgt ccgattcgat cgcgacgatg acgaagtcct tctcgcccat
     2821 gtcctcggcg cgcagcggcc ggtcgaacgt gacggtgacg gtcgggccct cctcgtcgtg
     2881 gcctgcggtc acgctgtgga tcgacagccg ccggtcgagg tcgtcgcggt cgcgccaggt
     2941 ccagtcgtct gcgagcgcga ggccggggag caggacgaag aggacggtcg ctgcgccgag
     3001 gagccgcgca cgtaggttcg gcatcgagta gttcccttcg cccggcgagc ggtagccacg
     3061 gggcaagtga cgcgagccgg taggtagtcc gccccctcgt cgctaagcca acaggactag
     3121 cccgcgaggg tcaatggggt tgggggccgc tcgcgttggt ccgagtgccg ctttccggga
     3181 ccgagcccaa cccgaaggcc ggagccgggg gctaggcccc cttctctacc tccactatcc
     3241 ggcccaagag gccgtttatg aagcggcccg agtcctctgt cgagtaggac ttcgccagct
     3301 ctactgcttc gtttatccct actgctaccg ggatgtcttc acgccatagc agctcgaaca
     3361 gggctatccg cagcagggtc ctgtcgacca ccggcatcct ctcgatcgcc cagcggtctg
     3421 cgtgcgctgc tatcaacacg tctatgtccg cgtggtgctc ttggaccccc gctaccaggt
     3481 ccgccgcgta ggccagggcc tcctcggtcg gctcgtccgg cggctcctcg gagtccgaag
     3541 ccgtcgccac cacccagcct ccggagcggc gggcctccaa ggcctcgctc ggcagctcgt
     3601 cgcggatctc ggcctcgtag aggacgtcca gcgcgaggcg tcgcgccgtg cggcgcgcct
     3661 tcacgagcgg gatggtgtca cccctgcacc ctcgactggt attcgccagt gcgcgtgtcg
     3721 accttgatcc tctcgccctt ctctacgaag agcgggacct gcaccaccgc gcccgtctcg
     3781 accgtcgcgg gcttgagcgc gcccgacacc cggtcgccct tgtgacccgg ctcggtgtcg
     3841 accacctcga gctccatcga cacgggcatc tccgcgccca ccggggtccc ctcgtacacc
     3901 gggaccatgc agatcgtcga gtccttgagg aacttgcgag agtcaccgag cagcgcgggg
     3961 tcgacgtgca cctgctcgta cgactcgttg tccatgaaca cgagcccggc gtcgtcctcg
     4021 tagaggtact gcatctcgcg cttgtcgaga acggcgaggt ctaccttctc gtcggcgcgg
     4081 aacgtcttct ccgtgaccgc gccgttcttc aggttcttga gcttcgtctt gacgaaggcc
     4141 tggcccttgc ccggcttgac gtgttggtag tcgacgatcg tgaacagtcc gttgtcgagg
     4201 ttcagggtct ggcccggcct catgtcgttg gtggagatca tcttctctac gccttcctat
     4261 tccgagatct cgatcagggt gtccttgggg gccttgccta gcacctcgca gccgtcgtcg
     4321 gtgacgagca cgcagtcctc gatgcggatg ccgcccgagc cggtctcgta gatgccgggc
     4381 tcgatcgtca tcacgtcgcc ggcgaccagg gtgtcctccg aggtcttgtg gaaacgcggg
     4441 gcctcgtgga tgtcgaggcc gacgccgtgg ccgagcccgt ggccgaacgt cgccccgtag
     4501 ccggcgtcgt cgatgaccgc gcgggcggcc gcgtccacgc cggagcacgc ggctcccgcg
     4561 cgcgtggcct cgatccccgc ggtctgggac gcgagcacga acccgtagac ctcgcggtgg
     4621 tggtcactcg cggagccgag cgctaccgtg cgcgtcaggt cggagcagta gccctgccag
     4681 cgcgcaccga agtcgagcag caccaggtcg ccgttctgca tctcgcggtc cgacggagtg
     4741 tggtggatgt gcgcggacag ggggccggtc ccgacgatgg gatcgaacga gatcgagtcc
     4801 gcgccccggc ggcgcatctc gacgtccagg tcgagcgcga cctcgcgctc ggtccggccg
     4861 gggcccagcc gctcgacgat ccagccgaac gcctcgtcgg cgatgcggac cgcctcgcgc
     4921 agcagcgaga cctcctccgc gtccttcgtg cgccgcaggt tctcgacgag gttcttcgtc
     4981 ggcgcaagct cgacctcgag cttgccttcg agctgcgcct tcgtcgcgac cgtcatcgtc
//
LOCUS       DASQDO010000040         1969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_495319, whole genome shotgun sequence.
ACCESSION   DASQDO010000040 DASQDO010000000
VERSION     DASQDO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495319"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VEV43_01250"
     CDS             complement(<1..734)
                     /locus_tag="VEV43_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYP22174.1"
                     /translation="MRNTGIKLAVFTVFTIFITFYLAAIIGNLSPFKDVYPVNAVFSD
                     ATGILKGDPVKIAGVTVGKVTGFEVERGQAIVTMEVEGHIDLPENVGADIKYRNLLGQ
                     RTINLVEPETPSTEPLGEDGTIPVENTTPALDLALVFNNLRPIIQSTNPEDINTVARA
                     MLKVFKGREDQFAQTLGNIGAVTQTLAGRDQRLARLVTDLNDLTKVLNSQKGSIRVSL
                     DRFTELMESLADVTPTVERVVDQLDDA"
     gene            complement(731..>1969)
                     /locus_tag="VEV43_01255"
     CDS             complement(731..>1969)
                     /locus_tag="VEV43_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HYP22175.1"
                     /translation="SKAGPPRRDRGKVGGTEITRLRHVITGVVALALMAGAVTLTGLA
                     LTGNLFRNSTNAFAEFENCGQGLREGGDVKLRGVLVGRIGPLERIEEGDCEVRLDLYP
                     ESIEQIPANAGAEIRAKTVFGEKWVELTYPDDPDEDAIAAGDVIPKERTLDPIEVETI
                     LNTGLPLLEAVDPENLAAALEALAEGFDGQEGEAGAAIREGIRALRPLNEDKALLARG
                     IDALDESSEVLDDVDEDLIEALDNLDSVNEFTVSNAALIEENLNRAPILLDELSVLFE
                     TRFGDLTKLVDRGATVIGIISARTDDLEKLLEVLPQFNSKWIRNLTPACPYRQVTTEP
                     GAEVGDRVPGRCWRVHNIISHSQGPYGPDDEQRPGPRKATPADYESLGLEPSGLSQLL
                     YSPALGALSEIDASRKGGRR"
BASE COUNT          265 a          669 c          674 g          361 t
ORIGIN      
        1 gcgtcgtcga gctggtcgac cacgcgctcg accgtcggag tgacgtcggc cagcgactcc
       61 atcagctcgg tgaatcggtc gagcgagacg cggatgctcc ccttctggga gttcaggacc
      121 ttcgtgaggt cgttcaggtc ggtcaccaac cgcgcgaggc gctggtcgcg gccggcgagc
      181 gtctgcgtga ccgcgccgat gttcccgagc gtctgcgcga actggtcctc gcggcccttg
      241 aagaccttca gcatcgcccg ggcgaccgtg ttgatgtcct cggggttcgt cgactggatg
      301 atcggacgga ggttgttgaa caccagcgcg aggtcgagcg ccggcgtcgt gttctcgacc
      361 gggatcgtcc cgtcctcgcc gaggggctcg gtcgacggcg tctcgggctc gacgaggttg
      421 atcgtgcgct gcccgaggag gttgcggtac ttgatgtcgg cgccgacgtt ctcgggcagg
      481 tcgatgtggc cctcgacctc catcgtcacg atcgcctgac cccgctcgac ctcgaacccg
      541 gtcaccttgc cgaccgtgac gccggcgatc ttgacggggt cgcctttgag gatgccggtc
      601 gcgtccgaga agaccgcgtt gaccgggtac acgtccttga acggcgacag gttgccgatg
      661 atcgcggcga gatagaacgt gatgaagatc gtgaagaccg tgaagaccgc gagcttgatc
      721 ccggtgttcc tcatcggcgc ccgcccttgc gtgacgcgtc gatctccgag agagcgccga
      781 gggccgggct gtacaggagc tggctcagcc cggacggctc gagcccgagg ctctcgtagt
      841 cggccggcgt cgccttgcgc ggccccggcc gttgctcgtc gtccggcccg tacgggccct
      901 ggctgtgcga gatgatgttg tggaccctcc agcagcggcc cggcacgcgg tcgccgacct
      961 cggcccccgg ctcggtggtc acctggcggt aggggcacgc cggcgtgagg ttgcggatcc
     1021 acttcgagtt gaactgcggg aggacctcga ggagcttctc gaggtcgtcc gtgcgtgccg
     1081 agatgatccc gatcaccgtg gcaccccggt cgacgagctt ggtgaggtcg ccgaagcgcg
     1141 tctcgaacag gaccgagagc tcgtccaaca ggatcggggc ccggttcagg ttctcctcga
     1201 tcagggccgc gttcgacacg gtgaactcgt tgaccgagtc gaggttgtcg agggcctcga
     1261 tcaggtcctc gtcgacgtcg tcgaggacct cggacgactc gtcgagggcg tcgatccccc
     1321 tcgcgagcaa cgccttgtcc tcgttcaggg gacgcagggc ccggatcccc tcgcggatcg
     1381 cggcgccggc ctcgccctct tgcccgtcga acccctcggc cagcgcctcg agcgcggccg
     1441 cgaggttctc cgggtcgacc gcttcgagca gcgggagccc ggtgttgagg atcgtctcga
     1501 cctcgatcgg gtccagcgtg cgctctttcg ggatcacgtc gcccgccgcg atcgcgtcct
     1561 cgtcggggtc gtcgggatag gtcagctcga cccacttctc gccgaagacc gtcttggcgc
     1621 ggatctcggc ccctgcgtta gcggggatct gctctatcga ctcggggtac aggtcgaggc
     1681 ggacctcgca gtcgccctct tcgatccgct cgagcgggcc gatgcgtccg acgaggactc
     1741 cgcggagctt gacgtcgccg ccctcccgca ggccctgacc acagttctcg aactccgcga
     1801 acgcgttcgt gctgttgcgg aacaggttcc ccgtcagggc gagcccggtc agcgttacgg
     1861 cgcccgccat gagcgcgagc gccaccactc ccgttattac gtgccgcagc ctggttatct
     1921 cggtgcctcc aactttcccg cggtcccgcc gaggcggccc cgccttgct
//
LOCUS       DASQDO010000041         7370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_311768, whole genome shotgun sequence.
ACCESSION   DASQDO010000041 DASQDO010000000
VERSION     DASQDO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311768"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..640)
                     /locus_tag="VEV43_01260"
     CDS             complement(149..640)
                     /locus_tag="VEV43_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22176.1"
                     /translation="MLDEPGHFFDMAIPPGGGGNGSLVFDLPLGAERRALRFHVAEGS
                     PGATIRLDPCPFLSRSGGGCYAEDDLGAEPEVAYPFTISGHRGGMALLNVCFDYRQWV
                     VVEPHPTEGMPADFTGHGVMTLTSPDRAEFRDNSGTLLTLAPTPENETHSGLCGSPNA
                     KKG"
     gene            complement(1207..1602)
                     /gene="rplL"
                     /locus_tag="VEV43_01265"
     CDS             complement(1207..1602)
                     /gene="rplL"
                     /locus_tag="VEV43_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYP22177.1"
                     /translation="MADTLTQDQVLDAIAGWTVLELSEFVKAFEDKFGVTAAAPVAVA
                     AAAGGGGGGDGAAAEEEKDEFDVILQAAGDKKIQVIKEVRALTSLGLKEAKDLVDGAP
                     KPVLEKVNKEQADEARGKLEGAGATVEVK"
     gene            complement(1607..2335)
                     /gene="rplJ"
                     /locus_tag="VEV43_01270"
     CDS             complement(1607..2335)
                     /gene="rplJ"
                     /locus_tag="VEV43_01270"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYP22178.1"
                     /translation="MARPEKTAAVEEITSRFGESSAALLTEYRGLTVTEIADVRNALR
                     EADADYKVMKNTLVRIAARDVGLEELAGLLTGPTAIAFCRGDAVAAAKALDEAAKRFP
                     VLVVKGGVLNGKILDGDQARALASIESREVLLSKIAMLVNQPAQQTVNVFSALLRNLG
                     SMLAQVVEKKESGELPGGTGDAATTEAPTETEAEVPQAAPEEAPEAEEPPAELPEAQD
                     QAAAAPEADATDATTDTTTDEEKE"
     gene            2537..2980
                     /locus_tag="VEV43_01275"
     CDS             2537..2980
                     /locus_tag="VEV43_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HYP22179.1"
                     /translation="MGTARRAGCDREREARMLRLAARQHGVVTRAQAIETGLTKSMVQ
                     TRVRTGRLRRIHPGVYCVGGSPATFEQRAFAAAAWAGPGAVLSHRSAGYLWGLVDRHP
                     PVDELWTPRRRTRPPAASVHTSRRIWLRAIEGGSGTSRSRPRHAR"
     gene            3028..3474
                     /locus_tag="VEV43_01280"
     CDS             3028..3474
                     /locus_tag="VEV43_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HYP22180.1"
                     /translation="MRAIVERRTTTAAIATRMREMSRQGVEGPRTLRRLLLQANGRCR
                     PGSPLERLVAGVLSRGGLPPVRREHPVYLEGGVYYLDFAWPHFRVGVEADSRRWHSDA
                     ASFERDLVRHNSLAAGGWRVLRVTEQQVRCDPAGVVDRVRGLLVRG"
     gene            complement(3464..4006)
                     /locus_tag="VEV43_01285"
     CDS             complement(3464..4006)
                     /locus_tag="VEV43_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HYP22181.1"
                     /translation="MSSEAARLLGLLAEPDRLKVVSAIVLGARTVDAIVAATELEPKA
                     VEVALSRLISGGLVADDRRNLSVDSEELRAAVRSAAEEREAGEKLGLPPVASDVLRRF
                     VRNGRLVSIPMARSKRLVILDHLSQEFEPGWRYTEKDVNEILGDYHEDVAALRRYLVD
                     EGFMARESGEYWRTGGTFTP"
     gene            complement(4012..4740)
                     /gene="rplA"
                     /locus_tag="VEV43_01290"
     CDS             complement(4012..4740)
                     /gene="rplA"
                     /locus_tag="VEV43_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041546211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HYP22182.1"
                     /translation="MSNKQGKRYAEAIKAVDRDKLYSPPEALKILKQFPSSKFDETVE
                     LSIRLGVDPRKADQMVRGAIALPKGTGKTMRVVVFAKGEKATAAREAGADEVGDDALG
                     ERIQGGWLDFDAVVASPDMMPTVGKLGRVLGPRGLMPNPKSGTVTDDVARAVRDIKGG
                     QVEYRTDRHGNLHLIIGKKSFDEGDLAENYAAVIEEVVRAKPAAAKGRYLKSITVSTT
                     MGPGIRIDPAKAKDVADDLEPASA"
     gene            complement(4800..5234)
                     /gene="rplK"
                     /locus_tag="VEV43_01295"
     CDS             complement(4800..5234)
                     /gene="rplK"
                     /locus_tag="VEV43_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009233246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HYP22183.1"
                     /translation="MGGARRKKVAQILKLQITGGQATPAPPVGPALGQAGINIMDFCK
                     QYNAATQSQTGTVVPVEITVYEDRSFTFVTKTPPAAVLLRQKAGVDKGSAEPNRTKVA
                     SVTKDQVREIAEIKMPDLNANDVEAAMKIVEGTARSMGITVS"
     gene            complement(5255..6070)
                     /gene="nusG"
                     /locus_tag="VEV43_01300"
     CDS             complement(5255..6070)
                     /gene="nusG"
                     /locus_tag="VEV43_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HYP22184.1"
                     /translation="MSETDLEQTQTEEAADVVAPQPEEGQTPDTSGPDTSGADNEELP
                     RLDPDPDPAADRTTSAADDPSVTPGEAEAKTDEEEGPSEAALYEETSIPPNERPGDWY
                     VIHTYAGYENKVKANLMSRISSMNVEDKVFEVVIPMEDVMEIKSGKKQVVQKKVFPGY
                     LLCRMYLEDDSWYVVRNTPGVTGFVGSGAKPTPLSDKEVDKILQVKPDQKKLKPRLEF
                     EEQESVRVTSGPFANFTGSISEINVDQSKLKVLVNIFGRETPVELGFDQVAKL"
     gene            complement(6072..6356)
                     /gene="secE"
                     /locus_tag="VEV43_01305"
     CDS             complement(6072..6356)
                     /gene="secE"
                     /locus_tag="VEV43_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HYP22185.1"
                     /translation="MNRQYKRLMKKEQERKATAPRPTAAAAANRPRTKPREFVKEVIG
                     ELRKVAWPTRKEVVAYSTVVFVSVVVIAALIFGMDYVFAKGVLFLYDIEG"
     gene            6827..7258
                     /locus_tag="VEV43_01310"
     CDS             6827..7258
                     /locus_tag="VEV43_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22186.1"
                     /translation="MRKRLPVVIAASAAVLTLVAGPAPARPAGARVHSLEYTGGGLIV
                     GSTRSTAVRVGDAIGAVEFRGGPERFVSIEIADAHGQPVTGVVVQPDGDEETHVCGTT
                     QKPVKVRPYENVTVFLMNGTCGDSGASVVTTGTVTATFTKR"
     gene            complement(7259..7332)
                     /locus_tag="VEV43_01315"
     tRNA            complement(7259..7332)
                     /locus_tag="VEV43_01315"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7296..7298),aa:Trp,seq:cca)
BASE COUNT         1055 a         2480 c         2596 g         1239 t
ORIGIN      
        1 cgtcgaacag ccggggtgcg gacacacgaa gagccagtat aggcaggcgc ccataccggc
       61 gtcaaataga acgctggcaa gccgagaagc tcgtaagaac tccctggtta tgaagttgcg
      121 gggactgtat tccccccata ggtcaaggtc aacctttttt cgcgttgggc gagccgcaga
      181 ggcccgagtg tgtctcgttc tccggagtcg gagccagagt caggagggtg ccggagttgt
      241 cccggaactc ggcccggtcg gggctcgtca gcgtcatcac cccgtgaccg gtgaagtccg
      301 cgggcatgcc ctcggtcggg tgcggctcga cgaccaccca ctggcggtag tcgaagcaga
      361 cgttcaacag cgccatgccg ccacggtggc ccgagatcgt gaacggatag gcgacctcgg
      421 gctcggctcc caggtcgtcc tcggcgtagc agccgccgcc gctgcggctg aggaaggggc
      481 acgggtcgag ccggatggtc gctccgggcg acccctcggc gacgtggaac cgcaaggccc
      541 gccgctccgc cccgagcggc aggtcgaaga cgaggctgcc gttcccccca cccccgggcg
      601 ggatcgccat gtcgaagaag tggccgggct cgtcgagcaa gatggcattc atagtccgcc
      661 cggacggcgg aagggcctca cgatcggtga cgagccgctg cgcactccaa gggagccgga
      721 cctcgacgtc ggactcgttg agaaccgaga agtaggtcca gcaatgcatc ccccggggcc
      781 gaatcccatc agtgtccaag tccccggtcg ggccggtccc gcactggacg ttccgaagct
      841 ggaaccgaac cccgcgatcc caaacgaagt caccaacccc gccggaagaa ggcccctgcg
      901 gccggggagc cggagcggtg tcatccccgc cggccagaac gaagacggct gcggccgcga
      961 ggacgagcgc gcccgccagc accacccgaa gtccagtccg agccacacgc gcctccccac
     1021 gaggtcccgg tccccaaggt actgcgacgg ttcggttgcg gcgagcgagg acggccccct
     1081 ccccggagga agacttaaag cctgcgtctg acggagggga gggggccgcc cgcagcggtc
     1141 gagacaacgg acgcgaaacg acccccggag actgaacccc gggggccgct cgaaaaacgc
     1201 ggggcgttac ttgacctcga cggtggctcc ggctccctcg agcttgccgc gggcctcgtc
     1261 ggcctgctcc ttgttgacct tctccaggac gggcttcggc gccccgtcga cgaggtcctt
     1321 ggcctccttc aggccgaggc tcgtgagggc acggacctcc ttgatgacct ggatcttctt
     1381 gtcgccggca gcctgaagga tgacgtcgaa ctcatccttc tcctcctcgg cggcggcacc
     1441 gtcgcctcca ccgccaccac cggcggcagc ggccactgcc acgggagccg cggcggtcac
     1501 gccgaacttg tcctcgaagg ccttcacgaa ctcggagagc tcgaggaccg tccaacctgc
     1561 gattgcgtcc agaacctggt cctgggtgag agtgtctgcc atctcgctac tccttctcct
     1621 cgtccgtggt ggtgtcggtg gtcgcgtcgg ttgcgtctgc ttcgggtgct gcggcggcct
     1681 ggtcctgggc ctcggggagc tcggccggtg gttcctcggc ctccggggcc tcctcggggg
     1741 cggcctgcgg gacctcggcc tcggtctcgg tcggggcctc ggtggtcgct gcgtcgccgg
     1801 tgccgccggg gagttcgccc gactccttct tctcgacgac ctgggcgagc atcgagccga
     1861 ggttgcgaag gagggccgag aagacgttga cggtctgctg ggccggctgg ttgacgagca
     1921 tcgcgatctt cgagagcagg acctcgcggg actcgatgct ggcgagggcg cgggcctggt
     1981 cgccgtcgag gatcttgccg ttgagcacgc cgcccttgac gacgaggacc gggaagcgct
     2041 tggcggcctc gtcgagcgcc ttggcggcag cgacggcgtc gccccggcag aacgcgatgg
     2101 cggtcgggcc ggtcaggagc ccggcgagct cctcgagccc gacgtcccgg gccgcgatgc
     2161 ggaccagggt gttcttcatc accttgtagt ccgcgtcggc ctcgcgcagg gcgttgcgta
     2221 cgtcggcgat ctcggtgacg gtgaggccgc ggtactcggt gaggagggcc gcgctcgact
     2281 cgccgaagcg ggaggtgatc tcctcgaccg ctgcagtctt ctcgggtctc gccattgcgt
     2341 ctccttgtcc gtgggcacga aaaaagcggc cgcccagtgc aggcgaccgc gcaaggaagc
     2401 gcaacgatcc cgcctgcgcc ggatgattaa gggcggaccc cccggcggtc tagggcaacg
     2461 ggggcaggat atcaggacgc gggaaaagtg acctgcgcca caccccaacc cggccctctc
     2521 tccgattctg tgtcacatgg ggacggctag gcgtgcggga tgtgacagag aacgggaggc
     2581 gcggatgctc cggctcgccg cacggcagca cggtgtcgtg acgagggccc aagcgatcga
     2641 gaccggcctg accaagtcga tggtccagac cagagtgcgc accgggcgcc tccgccggat
     2701 ccaccccggc gtctactgcg tcggcggcag ccccgcgaca ttcgagcagc gggcgttcgc
     2761 ggcggcggcg tgggcggggc ccggggcggt tctgtcccat cgctccgccg gctacctgtg
     2821 gggcctcgtc gaccggcatc cgccggtgga cgagctctgg accccgcgca gacggacccg
     2881 gcctccggcg gcgtctgtgc acacttcacg aaggatctgg ctgcgcgcga tcgagggcgg
     2941 ctccggaaca tcccgatcac gtccccggca cgcacgttga tcgacctcgc cgcggtcttg
     3001 cccccgccgc tcgtagaagc ggcgctcatg cgggcgatcg tcgagaggcg gacgaccacc
     3061 gcggcgatcg cgacacgcat gcgcgagatg tcgcggcagg gagtcgaggg accccggacg
     3121 ctgcggcggc ttctgctgca ggcgaacggg aggtgccgtc ccggctcacc gttggagcgg
     3181 ctcgtcgcgg gcgtcctctc gcggggtggg ctgccgcccg ttcggcggga gcatcccgtc
     3241 tatctcgaag gcggcgtgta ctacctcgac ttcgcctggc cgcacttccg tgtcggcgtc
     3301 gaggccgact cgcggcggtg gcactcggat gccgcgtcgt tcgagcgcga tctcgtcagg
     3361 cacaactcgt tagccgcggg cgggtggagg gtgctgcgcg tcaccgagca gcaggtgcgg
     3421 tgcgatccgg cgggggtcgt cgatcgcgtg cggggtttgc tcgtcagggg gtgaaggtgc
     3481 cgcccgtgcg ccagtattcg cccgactccc tcgccatgaa gccttcgtct accaggtagc
     3541 ggcgcagggc cgccacgtct tcgtggtagt cgcccaggat ctcgttcacg tccttctcgg
     3601 tgtagcgcca gcccggctcg aactcctggc tcaggtggtc caggatcacg agccgcttcg
     3661 agcgcgccat cgggatgctc accagccggc cattgcggac gaacctgcgg agcacgtcgc
     3721 tcgcgaccgg ggggaggccc agcttctcgc ccgcctcccg ctcctccgcc gccgagcgga
     3781 ccgcggcccg cagctcctcg gagtcgaccg acaggttgcg gcggtcgtcc gcgacgaggc
     3841 cgccggagat gagacgcgac agcgcgacct ccaccgcctt cggctcgagc tcggttgccg
     3901 cgacgatcgc gtcgaccgtg cgcgcgccga ggacgatcgc ggagacgacc ttcagacgat
     3961 cgggctcggc gaggagcccc aggagccggg ccgcctcgga ggacacgagc tctaggccga
     4021 ggcgggctcg aggtcgtcgg cgacgtcctt ggccttcgcc ggatcgatcc ggatcccggg
     4081 gcccatcgtc gtcgagaccg tgatgctctt gaggtagcgc cccttcgcgg cggcgggctt
     4141 cgcgcggacg acctcttcga tgaccgcggc gtagttctcg gcgaggtcgc cctcgtcgaa
     4201 cgacttcttg ccgatgatca ggtgaaggtt gccgtgacgg tcggtgcggt actcgacctg
     4261 gccgcccttg atgtcgcgga cagcgcgggc gacgtcgtcg gtgaccgtgc cggacttggg
     4321 gttcggcatg agcccgcggg ggccgaggac gcggccgagc tttccgacgg tcggcatcat
     4381 gtcggggctc gcgacgactg cgtcgaagtc gagccacccg ccctggatcc gctcgccgag
     4441 cgcgtcgtca ccgacctcgt cggcgcccgc ctcgcgagcg gcggtcgcct tctcgccctt
     4501 cgcgaacacg accacgcgca tggtcttgcc ggtccccttg gggagcgcga tcgcaccgcg
     4561 caccatctgg tcggccttgc gggggtcgac gccgagccgg atgctcagct cgacggtctc
     4621 gtcgaacttc gacgacggga actgcttgag gatcttcagc gcctcgggcg gcgagtagag
     4681 cttgtcgcgg tcgacggcct tgatggcctc cgcgtagcgc tttccctgct tgttcgacat
     4741 gtcgttcctc ctcgtggtct gcggcgagct cggcccgcct cccactcgat aggggtctgt
     4801 tagctgacgg tgatgcccat gctgcgggcg gtgccctcga cgatcttcat cgcggcctcg
     4861 acgtcgttcg cgttgaggtc gggcatcttg atctcagcga tctcgcgcac ctggtccttc
     4921 gtcaccgacg cgaccttcgt gcggttgggc tcggccgagc ccttgtcgac gccggccttc
     4981 tggcgcagga gcaccgcggc cggcggggtc ttggtgacga acgtgaacga gcggtcctcg
     5041 tagaccgtga tctcgaccgg cacgacggtg cccgtctggc tctgcgtcgc agcgttgtac
     5101 tgcttgcaga agtccatgat gttgatgccg gcctggccga gcgccgggcc cacgggggga
     5161 gccggcgtcg cctgcccgcc cgtgatctgg agcttcagaa tctgcgcgac cttcttgcgg
     5221 cgtgcgccac ccatctatgc gtctccgttg agtcctagag cttggcgacc tggtcgaagc
     5281 cgagctcgac cggggtctct cgaccgaaga tgttgacgag gaccttgagc ttcgactggt
     5341 cgacgttgat ctccgagatc gagccggtga agttcgcgaa ggggccggag gtgacgcgga
     5401 cgctctcctg ctcctcgaac tcgagccgcg gcttgagctt cttctggtcc ggcttgacct
     5461 ggaggatctt gtcgacctcc ttgtccgaca gcggcgtcgg cttcgcgccc gaccccacga
     5521 acccggtcac gcccggcgtg ttgcgcacga cgtaccagga gtcgtcctcg aggtacatgc
     5581 ggcacagcag gtagccgggg aacaccttct tctggacgac ctgcttcttg ccgctcttga
     5641 tctccatgac gtcttccatc gggatgacga cctcgaagac cttgtcctcg acgttcatcg
     5701 aggagatgcg cgacatcagg ttcgccttga ccttgttctc gtagccggcg tacgtgtgga
     5761 tgacgtacca gtcgccgggc cgctcgttcg gagggatgga cgtctcctcg tagagcgcgg
     5821 cctcggaagg gccttcttct tcgtcggtct tggcctcggc ctcgccgggc gtcacgctcg
     5881 ggtcgtcggc ggccgacgtc gtgcggtccg cggcgggatc cgggtcggga tcgagccggg
     5941 ggagctcttc gttgtcggcg ccggacgtgt cggggccgga cgtgtcgggc gtctggcctt
     6001 cctccggctg gggtgccacg acgtccgcgg cctcctcggt ctgggtctgc tcgagatcgg
     6061 tctcgctcac gtcagccctc gatgtcgtag aggaacagca cgcccttggc gaagacgtag
     6121 tccatcccga agatgagggc ggcgatcacg acgacgctca cgaagacgac cgtcgagtag
     6181 gcgacgacct ccttgcgggt cggccatgcc accttgcgga gctcgccgat gacctccttg
     6241 acgaactcgc gcggcttggt ccgcggccgg ttcgccgccg cggccgcagt cgggcgcggc
     6301 gcggtggcct tgcgctcctg ctccttcttc atgaggcgct tgtactgcct gttcacgaga
     6361 gccctttctc gatccccccg gacgcggttc gtaagggtcg gtacctcagg gggggacgaa
     6421 cggtcagatt agcacgccgg ggcctcgcag gccctggccg cgacgttgct aggcgggctc
     6481 gtcgtgcgcg ggcagcaggg gggtcgagca gaccgggcac accggagcct cgtcctctcc
     6541 gaggtagacg gtccgctggc agaccgtgca gaagttctcc gtggccaagg ttcctccgat
     6601 accggtagca gttattgggc gggggccatc gagaagaagc cgcggtcgac ggcgagatct
     6661 tttcgaggat cgggagcccg acgcctgctt cgaggctccg gctcggcccc cgcacccgga
     6721 atatacgccg ctcgtctgct tcgtcctgct cgaaggggtt acgggtccgg gggccgaacg
     6781 tattcagcag acgaaatccc gttcggtcgc ctcgcaggag gaagagatga ggaagcgact
     6841 cccggtggtg atcgcagcga gtgccgcggt gctgactctc gtcgccggcc ccgcaccggc
     6901 acggccggcg ggggcgcgcg tgcactccct cgagtacacg ggcggcggcc tgatcgtcgg
     6961 ctccacgcgc agcaccgccg tacgcgtcgg cgacgcgatc ggtgcggtcg agttccgcgg
     7021 ggggcccgag cgcttcgtct cgatcgagat cgccgacgct cacggccagc ccgtgacggg
     7081 cgtcgtggtc cagccggacg gcgacgagga gacgcacgtc tgcgggacga cccagaagcc
     7141 ggtgaaggtc cgtccctacg agaacgtgac ggtgttcctg atgaacggga cgtgcgggga
     7201 ctcgggggcc tcggtggtga cgacgggaac ggtgacggcg acgttcacga agaggtagca
     7261 gggcgggagg gactcgaacc cccgaccact ggttttggag accagcgctc taccaaactg
     7321 agctaccgcc ctttgatacg cgcccagtct agtgcgcgac tcaggccgtg
//
LOCUS       DASQDO010000042        12347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_189512, whole genome shotgun sequence.
ACCESSION   DASQDO010000042 DASQDO010000000
VERSION     DASQDO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189512"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..576
                     /gene="rpiB"
                     /locus_tag="VEV43_01320"
     CDS             124..576
                     /gene="rpiB"
                     /locus_tag="VEV43_01320"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HYP22187.1"
                     /translation="MKIALGSDHAGYALKSALGHRLKELGHEVLALGTDSEERADYPR
                     FCAAVGRAVTSGDADRGIVMGGSGQGEAISANKVRGVRAALCLDEYTARMAREHNDAN
                     VLALGARIVATAMAFRITELFLETPFEGGRHVPRVEQIAAIEQEECDR"
     gene            573..1838
                     /gene="glyA"
                     /locus_tag="VEV43_01325"
     CDS             573..1838
                     /gene="glyA"
                     /locus_tag="VEV43_01325"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYP22188.1"
                     /translation="MSNPGWADWAAVEKVDPEAAEAILAEVHRENTKIELIASENFSS
                     TAVLAALGTPLTNKYAEGYPGRRYYGGCEHVDVVETLAIERAKALFGADHVNVQPHAG
                     AVANVAAYFAFMEPGDTLLGMSLAHGGHLTHGFPVSFSGKIFRAVSYGVEEDTELIDL
                     DKVRAVALKEKPKMIVAGASAYSRIWDFEAFRSIADECGAMFLVDAAHFIGLVAGGAH
                     PNPVPHADIVTCTTHKTLRGPRGAMIMCKEEYASAIDKSVFPGWQGGPLMHSIAAKAV
                     AFAEAASDGFRDYAHQTVRNAQAMAATLSEEGLRIVSGGTDNHLMLVDLRPVGITGRD
                     AEARLDAVGITVNKNAIPYDPEKPFVASGIRIGTPAMTTCGMKEDEVAEVARLIARVL
                     KDDGEATASDVAGRVRELTTRFRPYPDVA"
     gene            1843..2955
                     /locus_tag="VEV43_01330"
     CDS             1843..2955
                     /locus_tag="VEV43_01330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HYP22189.1"
                     /translation="MGPYLLIGLVAAVVTFAATPLVRWGSLRFGAIDHPNDRKVHAHP
                     TPTLGGLALFIGLLVAGAVASQMSEFETVFTGSSEVLGILAGGVIIFALGVVDDLRDL
                     PAPVKLAGQVFASGILFLSGVKMQFVLLPGGAYNLSDDVSVLVTVLWLVAMINAVNLV
                     DGLDGLAAGIVAIAAASFFVYTYDFAVRGFYAGVPTAPLVAMMIVGISLGFLRHNFHP
                     AKIFMGDSGSMLLGFVLGAATVAGIGHAPQINPSVPGTQVFFAYFPLVIPLMVIALPI
                     VDALLAIVRRARRRRSVFHADKEHLHHRLMDLGHGHRQAVLVMYLWSALAAGAGLSFR
                     FVNRNEFVYGLPIAVGAIVLYTLFPVLTKAIQDRLG"
     gene            2965..4002
                     /locus_tag="VEV43_01335"
     CDS             2965..4002
                     /locus_tag="VEV43_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22190.1"
                     /translation="MKRIAVCLTLVLSSLVAPASGAGPRTCERIAFVFYGPAGNRVFT
                     VRPDGSALRPVGERATTTTRFEPRWAADGRTLTWFESGGGDSAVWVADREGRSPRRVT
                     TRDEYAFDARLSPDGERVAFVRMDGQFKIVVLDLETEEERVVATGVKPEWSPDGSRIA
                     YASMIDPETSTSDIRVVSADGTENVAVTSEPTTDDFNPSWAPGGDAIAFERRPEEQDG
                     PRDYGDVWIAQADGSREERLTTAYTERQGFLNPRFSPDGLHIAATFQNVDRTEVWVMD
                     HDGGNLHRVGESRARTATPSWSPDGRRLAYREGRAIVASALDGSRRRVVWRPETKRGA
                     AGYPEWGPCPG"
     gene            4258..4506
                     /locus_tag="VEV43_01340"
     CDS             4258..4506
                     /locus_tag="VEV43_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HYP22191.1"
                     /translation="MSKVPGRGQSTQAGAGMGMSLAFEFAGAVALFWFFGLLVDRWLG
                     IEPIGQVVGGVLGWIGGILHVYYSVKSRGGFEQGRGTR"
     gene            4503..4913
                     /locus_tag="VEV43_01345"
     CDS             4503..4913
                     /locus_tag="VEV43_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22192.1"
                     /translation="MIELGMVGRMARRALLLTPVLVAILWLVDGPKWGLSGAAGVAMT
                     LGNLWLSARILGGVAERQPKLLLPAGLAAFMLGLFLLTMVALALRELDLVYFPVTGFT
                     LIGCHLGLVLWEAAGAYKHTGTETNPLSAADTRS"
     gene            4918..5766
                     /gene="atpB"
                     /locus_tag="VEV43_01350"
     CDS             4918..5766
                     /gene="atpB"
                     /locus_tag="VEV43_01350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HYP22193.1"
                     /translation="MELDVNHLFFWDPILFEGTPFAFNRVTLLMVVAGLACLAFFWAG
                     SKGRLVPKGFFQNAAEQGYMFVRNQIAVDVIGPKDGPKYAPYLASLFFFIFFCNLLEI
                     VPGINFPVTSRMAVPAALALLTYVIFVVVGFKKQGFRFLKDTLFPPGVPMAVKPLLAV
                     IELASVFFIRPLTLAIRLLANMMAGHVLLTIFFLFTHDFLLDDVGISAPLGLVTFVVA
                     CGLLVFEMMVISIQAYIFTLLTAFYISESIHGHGDHADTSHDEAHKVETQKPLTEGIE
                     APATAA"
     gene            5798..6052
                     /gene="atpE"
                     /locus_tag="VEV43_01355"
     CDS             5798..6052
                     /gene="atpE"
                     /locus_tag="VEV43_01355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HYP22194.1"
                     /translation="MTDLITLAQEGGLTDEGVATIGKGLVYGLAAIGPGIGVGLVFSS
                     AIEAMARQPEAAGETRTTMFLGFALIEALALIGFVLSFII"
     gene            6067..6654
                     /gene="atpF"
                     /locus_tag="VEV43_01360"
     CDS             6067..6654
                     /gene="atpF"
                     /locus_tag="VEV43_01360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HYP22195.1"
                     /translation="MIDKVHSLLLLAAEETEHAEEPSGVDLVLPAVEELIWGFIVFGV
                     VLLILNKILFPKIRQGIEAREQRIQADLEAAESSKASAQTELEQYRAQLADARSEANR
                     IIEEARQQADQVRKDNEAKARKDAEDIVARAQETINAERTRTIQELQGTIAAISIDLA
                     EKVVGRSLDNQAQREFVDAYIRDVAGMGTNGGSTN"
     gene            6654..7190
                     /gene="atpH"
                     /locus_tag="VEV43_01365"
     CDS             6654..7190
                     /gene="atpH"
                     /locus_tag="VEV43_01365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HYP22196.1"
                     /translation="MATDELIHGYAEALFSIVRAEGDLDRVEDELFHFGKLLESNHEL
                     KQALSDKSIEREQRAKVLQSILAGKVSPHTLGLLEFIVAQGRARQLPQILEELSDLAA
                     QARSSVVAEVRSAVPLDESQQAQLAAALTKATGKKVEVKVLVDPSVIGGIVSKVGDTV
                     IDGTVRRRLEQLKEQVRG"
     gene            7190..8869
                     /gene="atpA"
                     /locus_tag="VEV43_01370"
     CDS             7190..8869
                     /gene="atpA"
                     /locus_tag="VEV43_01370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HYP22197.1"
                     /translation="MANGSDATTLSISPEEISSALKRHVTDFRPTTEREEVGIVTEVG
                     DGIARISGLPGVMANEMLEFPGGVVGLAMNLDEDEIGCVILGEASHVEEGNSVKQTGN
                     ILSVGVGDELLGRVVDALGNPVDGKGPIRTSARRPLEVQAPGVTARQPVTEPLQTGIK
                     AIDSMTPVGRGQRELIIGDRQTGKTAIAIDTIINQRQMWGTDKAVKCIYVAVGQKAST
                     VAEVVGALEEAGAMEYTVVVNAAASDPAPFQFLAPYSGCAMGQHWMENGEHSLIVYDD
                     LSKQAIAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERSAKLSDANGGGSLTALPIIE
                     TKGGDVSAYIPTNVISITDGQIFLESDLFFSGVRPAINVGISVSRVGGNAQVKAMKQV
                     AGRLRLDLAQYRELEAFAEFGSDLDKASQAQLDRGARIVEILKQPQYTPFPVEDQIIS
                     IWAVTNGFADDVPIEKVSAFEAALLDHVRTRHSSLAESIRTSGKLDETAEESLKKAVG
                     EFKSTWGDTTETTASTAGVPDAGGGGAVNAATPGDTTAQLDTAGTDSVSGE"
     gene            8873..9760
                     /locus_tag="VEV43_01375"
     CDS             8873..9760
                     /locus_tag="VEV43_01375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HYP22198.1"
                     /translation="MAQLRDIKRRIGSVQSTQKITRAMELIASSRIIKAQQRVDAARP
                     YAEEMRSLMASVAKNAGSIDHPLLKQRDTVGTVGTIVITADRGLAGAYNSNVIRAAER
                     DMRDHEQTRLFVAGKKGVSYFRFRGYDLTESWQGMSDQPRIDDARAVAKSAADAFTNG
                     DVDEVRLAYTKFESALTQRPTVLKLLPVPRADLDESQSSADGPKPSYVFEPAPEDILG
                     YLLPRYIEGAVYQGMLEAAASEHAARRRAMKAATDNADELIENLTRVYNQARQAEITT
                     EIMEVVGGAEALSAGRAND"
     gene            9770..11209
                     /gene="atpD"
                     /locus_tag="VEV43_01380"
     CDS             9770..11209
                     /gene="atpD"
                     /locus_tag="VEV43_01380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HYP22199.1"
                     /translation="MAVTEERLSQPKTSEGRVMTVTGPVIEVEFPPDALPEINYALEV
                     ERTIAGKTDTITAEVSQHIGASVVKAICMKATDGLVRGTKVLNTGLPITVPVGPSTLG
                     HVYNVLGEPVDESSVPEGVERWPIHRQPPKFEDLEPKTEMFETGIKVIDLLEPYVQGG
                     KIGMFGGAGVGKTVVIQEMIYRVAEQHGGVSVFAGVGERTREGNDLWLEMKESGVLEK
                     TALVYGQMDEPPGVRLRVALSALTMAEYFRDVEHQDVLLFIDNIFRFVQAGSEVSTLL
                     GRMPSAVGYQPTLSEEMGELQERITSTKGRSITSLQAIYVPADDITDPAPHTAFAHLD
                     ATTVLSRQIASLGIYPAVDPLDSTSRILDPRYVGDEHYNVALTVQRILQRYKDLQDII
                     AILGIDELSEEDKIIVQRARKIQRFLSQPFFVAEQFTGLPGKYVPVADTIASFKGIAE
                     GEYDHLPEQAFYLVGGIEEAEAKAKELSE"
     gene            11209..11607
                     /gene="atpC"
                     /locus_tag="VEV43_01385"
     CDS             11209..11607
                     /gene="atpC"
                     /locus_tag="VEV43_01385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HYP22200.1"
                     /translation="MQVSIVSPEEEVWRGDADMVVARSPEGEFGIMRGHIPFLAALVP
                     GRVAVVTGNQREEFFVPGGFLEASGTRDDYHVIILADSAENLADIDVAEARRIAEEAS
                     RRRDEDTDERAEAEMRVALARVEIAESRGH"
     gene            11611..11820
                     /locus_tag="VEV43_01390"
     CDS             11611..11820
                     /locus_tag="VEV43_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22201.1"
                     /translation="MTDAQGELRNRIFCSSCGSSTARRNGLAPLGWQEMPDANNRRIA
                     LCPECVRRNLWLIEARLDIDPGSGF"
     gene            complement(11876..12085)
                     /locus_tag="VEV43_01395"
     CDS             complement(11876..12085)
                     /locus_tag="VEV43_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22202.1"
                     /translation="MNKIRTGVVAGVIGALSLAGAVPAHASTADRDACQIMGPQYVEG
                     VVGCACVVIALLAPSPVPDVKVCSR"
     gene            complement(12110..>12347)
                     /locus_tag="VEV43_01400"
     CDS             complement(12110..>12347)
                     /locus_tag="VEV43_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22203.1"
                     /translation="RKAAVTAFAAATLLAGTAVPARAAATDGCFLGSPNIVRDVTDCA
                     FYVVETVNGCFIGSPNIIRDVTDCAAYVVDRLTV"
BASE COUNT         2006 a         4222 c         4238 g         1881 t
ORIGIN      
        1 aggcgggggt gagggtcctg gcccgggtcc tgtgtggaga gcgcgaatga atcggcgact
       61 cgaccctcgg cggagccgtt cgatagccaa aaggcgctcg gctcggtctt ctaggatgcg
      121 ggggtgaaga tcgccctggg atccgaccac gccgggtacg cgctcaagtc cgccctcggc
      181 caccgcctga aggagctcgg ccacgaggtc ctcgccctcg gcacggattc ggaggagcgc
      241 gcggactacc cccgcttctg cgcggcggtc ggccgcgcgg tcacgtcggg cgacgccgac
      301 cgggggatcg tcatgggcgg cagcgggcag ggcgaggcga tctcggcgaa caaggtccgg
      361 ggagtgaggg ccgccctgtg cctcgacgag tacacggcgc gcatggcccg cgagcacaac
      421 gacgcgaacg tgctcgccct cggggcccgc atcgtcgcga ccgcgatggc gttccggatc
      481 accgagctgt tcctcgagac gccgttcgag gggggccgcc acgtgccgcg cgtcgagcag
      541 atcgcagcca tcgagcaaga ggagtgtgac cggtgagcaa tcccggatgg gccgactggg
      601 ccgccgtcga gaaggtcgat cccgaggccg ccgaggccat cctcgccgag gtccaccgcg
      661 agaacacgaa gatcgagctg atcgcgtccg agaacttctc gtcgaccgcg gttctggccg
      721 cgctcggcac gccgctgacg aacaagtacg ccgagggcta ccccggccgc cgctactacg
      781 gcggatgcga gcacgtcgac gtcgtcgaga cgctggcgat cgagcgggcg aaggctctct
      841 tcggcgccga ccacgtgaac gtccagccgc atgccggggc cgtggcgaac gtcgcggcgt
      901 acttcgcctt catggagccg ggcgacacgc ttctcggcat gtcgctcgcg cacggcgggc
      961 acctcacgca cggcttcccg gtgagcttct ccggcaagat cttccgtgcc gtgtcgtacg
     1021 gcgtcgagga ggacaccgag ctgatcgacc tcgacaaggt gcgcgccgtc gccttgaagg
     1081 agaagccgaa gatgatcgtc gccggcgcct ccgcgtactc gcgcatctgg gacttcgagg
     1141 ccttccgctc gatcgccgac gaatgcgggg cgatgttcct cgtcgacgcg gcccacttca
     1201 tcgggctcgt cgccggcgga gcgcacccga acccggtccc gcacgccgac atcgtgacgt
     1261 gcacgacgca caagacgctg cgggggccgc gcggggcgat gatcatgtgc aaggaggagt
     1321 acgcgtccgc gatcgacaag tcggtgttcc ccggctggca gggcgggccg ctgatgcatt
     1381 cgatcgcggc gaaggccgtg gcgttcgccg aggccgcgag cgacgggttc cgcgactacg
     1441 cgcaccagac cgtccgcaac gcgcaggcga tggcggcgac gctgtcggag gagggcctgc
     1501 gcatcgtctc cggcggcacg gacaaccacc tgatgctggt ggacctccgt cccgtgggga
     1561 tcacggggcg cgacgccgag gcccggctcg acgccgtcgg catcaccgtg aacaagaacg
     1621 cgatcccgta cgaccccgag aagcccttcg tcgcgtcggg catccgcatc ggcacgcccg
     1681 cgatgacgac ctgcgggatg aaggaggacg aggtcgcgga ggtcgcgcgc ctgatcgccc
     1741 gggtgctcaa ggacgacggc gaggcgacgg cctccgacgt cgccggccgc gtccgcgagc
     1801 tgaccacccg cttccgcccg tacccggacg tagcctgagg ctgtgggccc ctacctcctg
     1861 atcggcctcg tggccgccgt cgtgacgttc gcggcgactc cgctggtgag gtgggggtcg
     1921 ttgcggttcg gtgcgatcga ccatccgaac gaccggaagg tccacgcgca cccgacgccg
     1981 acgctggggg gcctcgctct gttcatcggc ctgctcgtcg cgggagccgt cgccagccag
     2041 atgagcgagt tcgagacggt gttcaccggc tcgtccgagg ttttggggat cctggcgggc
     2101 ggcgtgatca tcttcgccct cggcgtggtc gacgacctga gggacctccc ggcccccgtg
     2161 aagctggccg gccaggtctt cgcctccggc atcctcttcc tgtccggcgt gaagatgcag
     2221 ttcgtgctgc tgcccggcgg cgcgtacaac ctctccgacg acgtgtcggt gctcgtcacg
     2281 gtgctgtggc tcgtcgcgat gatcaacgcg gtgaacctcg tcgacgggct cgacggcctc
     2341 gccgcgggga tcgtcgcgat cgcggcggcg tcgttcttcg tctacacgta cgacttcgcc
     2401 gtccgggggt tctacgccgg cgtcccgacg gcgccgctcg tcgcgatgat gatcgtcggg
     2461 atctcgctcg ggttcctgcg gcacaacttc cacccggcga agatcttcat gggcgactcg
     2521 gggagcatgc tgctcgggtt cgtgctcggc gcggcaacgg tcgccgggat cggccacgcg
     2581 ccgcagatca acccgtcggt gccgggcacg caggtcttct tcgcctactt ccccctggtg
     2641 atcccgttga tggtcatcgc gctgccgatc gtcgacgcgt tgctcgcgat cgtgcgccgg
     2701 gcgcggcgcc gtcgcagcgt cttccacgcc gacaaggagc acctgcacca ccgcctcatg
     2761 gacctcggcc acggccatcg ccaagcggta ttggtgatgt acctgtggtc ggcgctggca
     2821 gcgggggccg ggctgtcgtt ccgcttcgtg aaccggaacg agttcgtcta cggcctgccg
     2881 atcgcagtag gggcgatcgt gctgtacacg ctgttcccgg tgctcacgaa ggcgatccaa
     2941 gaccgcctcg gataggggct tcgcgtgaag cgcatcgcgg tctgcctgac gctggtcctg
     3001 tcgagcctcg tcgctccggc gtcgggcgcg ggcccacgca cgtgcgagcg catcgcgttc
     3061 gtcttctacg gccccgccgg caacagggtc ttcacggtcc ggcccgacgg gagcgcgctc
     3121 cggcccgtcg gcgagagggc gacgacgacg acccggttcg agccgcgctg ggcggccgac
     3181 ggtcgcacgc tgacctggtt cgagtccggc ggcggcgatt cggccgtgtg ggtagcggat
     3241 cgagagggcc ggtcgccgcg ccgcgtcacg acgcgggacg agtacgcgtt cgatgcgcgg
     3301 ctgtccccgg acggagagcg cgtcgcgttc gtgcggatgg acgggcagtt caagatcgtc
     3361 gtcctcgacc tggagaccga ggaggagcgc gtcgtcgcta ccggcgtgaa gcccgagtgg
     3421 tccccggacg ggtcccgcat cgcgtacgcg tcgatgatcg acccggagac gtccacgagc
     3481 gacatccgcg tcgtgtccgc ggacggcacc gagaacgtcg cggtgaccag cgagccgacc
     3541 accgacgact tcaacccgtc ctgggccccc gggggcgacg ccatcgcgtt cgaacgccgc
     3601 cccgaggagc aggacggtcc ccgcgactac ggcgacgtgt ggatcgcgca ggcggacggc
     3661 tcccgagagg agcgattgac gaccgcgtac accgagcggc agggcttcct gaatccgcgg
     3721 ttctcgccgg acggcctgca catcgccgct acgttccaga acgtcgaccg caccgaggtc
     3781 tgggtgatgg accacgacgg agggaacctc caccgggtgg gggagagccg cgcccgcacc
     3841 gccacgccgt cgtggtcgcc cgacggccgg cgcctcgcgt accgcgaggg ccgcgcgatc
     3901 gtggcgtcgg cactggacgg gtcgcgccgg cgtgtcgtct ggcgcccgga gaccaagcga
     3961 ggggccgcgg gctaccccga atgggggccg tgccccggct gacccccgcc gcgtgcgcaa
     4021 cgcattgcgg aatccgccac ccactgcgca cgcaggcccc cggcaacagg tttcggcccc
     4081 ccaaaacctg acttttggcg agttgcaacg cggccctctc tggggtagct tgtgaaaagt
     4141 ttcacaagca aggagatgcg ttaggatgcc gttcctccca ctccagaacg cccccggagc
     4201 acgtctctgc ccagccgaga tcgggttgcc gtgggcctcg tcgcgggagg actccaggtg
     4261 agcaaggtgc ctggacgggg ccagagcacg caggcgggag cgggcatggg catgtccctc
     4321 gcgttcgagt tcgcgggtgc ggtggccttg ttctggttct tcggcctcct ggtcgaccgg
     4381 tggctcggca tcgagcccat cggacaggta gtgggcggcg tgctcgggtg gatcggcgga
     4441 atcctgcacg tctattactc ggtgaagagc cggggcggct tcgaacaagg gagagggacc
     4501 aggtgatcga gcttgggatg gtgggccgga tggctcgccg ggcactcctc cttactcctg
     4561 tcctcgtcgc catcctgtgg ctcgtcgacg ggcccaagtg gggtctctcc ggcgccgccg
     4621 gcgtcgccat gacgctcggc aacctctggc tctcggcccg catcctcgga ggggtggcgg
     4681 aacgccagcc gaagctcctc ctccccgcag gcctcgcggc cttcatgctc ggcctcttcc
     4741 tcctcacgat ggtcgccctc gccctgcgcg agctcgacct cgtctacttc cccgtcaccg
     4801 gcttcaccct catcggctgc cacctcggcc tggtgctgtg ggaggctgcc ggggcttaca
     4861 aacacaccgg aaccgagacg aatcccctga gcgcggccga cacaaggagt tgattggatg
     4921 gagctagacg tcaatcacct tttcttctgg gacccgatcc tcttcgaggg gacgccgttc
     4981 gcgttcaacc gcgtcaccct gttgatggtc gtggccggcc tcgcgtgcct ggccttcttc
     5041 tgggcgggat cgaagggccg gctcgtgccg aaggggttct tccagaacgc ggccgagcag
     5101 ggctacatgt tcgtccgcaa ccagatcgcg gtcgacgtga tcgggccgaa ggacggaccg
     5161 aagtacgcgc cgtacctcgc gtcgctgttc ttcttcatct tcttctgcaa cctgctcgag
     5221 atcgtccccg gcatcaactt cccggtgacg tcccggatgg cggtgccggc cgcgctggcg
     5281 cttctgacct acgtgatctt cgtggtcgtc gggttcaaga agcagggctt ccgcttcctc
     5341 aaggacacgc tgttcccgcc gggcgtgccg atggccgtca agccgctgct cgcggtgatc
     5401 gagctggcgt cggtgttctt catccgtccg ctcacgctcg ccatccggct cctcgccaac
     5461 atgatggccg ggcacgtgct gctgacgatc ttcttcctgt tcacgcacga cttcctgctc
     5521 gacgacgtcg gcatctcggc gccgctcggg ctcgtcacgt tcgtcgtggc gtgcggcctg
     5581 ctcgtcttcg agatgatggt catctcgatc caggcctaca tcttcacgct gctcacggcc
     5641 ttctacatct ccgagtcgat ccacggccac ggggaccacg cggacaccag ccacgacgaa
     5701 gcacacaagg tcgagacgca gaaaccgctc accgaaggta tcgaagcacc cgcaaccgca
     5761 gcctagagat cactacagaa agagggagga ccaacaaatg accgacctga tcacgctggc
     5821 gcaggaaggc ggcctcacgg acgagggcgt cgcgaccatc ggcaagggcc tcgtatacgg
     5881 cctcgccgcc atcggcccgg gcatcggcgt cggcctggtg ttctcgagcg ccatcgaggc
     5941 catggcccgt cagcccgaag ccgccggaga gacccggacg acgatgttcc tcggcttcgc
     6001 gctcatcgag gcccttgcgc tcatcggctt cgtcctctcg ttcatcatct aaggggggcg
     6061 taaggaatga tcgacaaagt ccattcgctc ctgctgctcg cagcggaaga gaccgagcac
     6121 gccgaggagc cttccggtgt cgacctcgtc ctccccgcgg tcgaggagct catctggggc
     6181 ttcatcgtgt tcggggtcgt gcttctgatc ctgaacaaga tcctgttccc caagatccgt
     6241 cagggcatcg aggcgcgcga gcagcggatc caggcagacc tcgaagccgc cgagtcgtcg
     6301 aaggcgtcgg cccagaccga gctcgagcag taccgggccc agctcgccga cgcgcgcagc
     6361 gaggccaacc gcatcatcga agaagcccgc cagcaggcgg accaggtccg caaggacaac
     6421 gaggccaagg cccgcaagga cgccgaggac atcgtcgccc gcgcgcagga gaccatcaac
     6481 gccgagcgga cgcggacgat ccaggagctc cagggcacga tcgccgcgat ctccatcgac
     6541 ctcgccgaga aggtcgtcgg ccgctcgctc gacaaccagg cgcagcgtga gttcgtcgac
     6601 gcgtacatcc gcgacgtcgc cgggatgggc acgaacgggg gcagcacgaa ctgatggcca
     6661 ccgacgagct cattcacgga tacgcggagg cgctcttctc gatcgtccgg gccgagggcg
     6721 acctcgaccg ggtggaggac gagctcttcc acttcggcaa gctgctcgag agcaaccacg
     6781 agctgaagca ggcgctgtcc gacaagagca tcgagcggga gcagcgggcg aaggtgctcc
     6841 agtcgatcct cgcgggcaag gtgagcccgc acacgctcgg gctcctcgag ttcatcgtcg
     6901 cccagggccg cgcccgccag ctgccgcaga tcctggagga gctctcggac ctcgcggcgc
     6961 aggcacgcag ctctgtcgtg gccgaggtcc gctcggccgt cccgctcgac gagtcgcagc
     7021 aggctcagct cgcagcggcg ctcacgaagg cgacgggcaa gaaggtcgag gtgaaggtcc
     7081 tggtcgaccc ctcggtcatc ggagggatcg tctcgaaggt cggggacacc gtcatcgacg
     7141 gcacggttcg acgccgtctc gaacagttga aggagcaggt gaggggctag tggcaaacgg
     7201 tagcgacgcc accacactgt cgatctcccc cgaggagatc tcttccgccc tcaagcgcca
     7261 cgtcacggac ttccgtccga cgaccgagcg cgaggaggtc gggatcgtca ccgaggtcgg
     7321 tgacggcatc gctcgcatca gcgggctgcc cggcgtcatg gcgaacgaga tgctcgagtt
     7381 ccccggcggc gtcgtcgggc tcgcgatgaa cctcgacgag gacgagatcg gttgcgtcat
     7441 cctcggcgag gccagccacg tcgaggaggg gaactcggtc aagcagaccg ggaacatcct
     7501 ctcggtcggc gtcggcgacg agctgctcgg ccgcgtggtc gacgcgctcg gcaaccccgt
     7561 cgacggcaag ggcccgatca ggacgtcggc ccggcggcct ctcgaggtcc aggcccccgg
     7621 cgtgaccgcg cggcagccgg tgacggagcc tctccagacc ggcatcaagg ccatcgactc
     7681 gatgacgccc gtcggccgtg gccagcgcga gctgatcatc ggcgaccggc agaccggcaa
     7741 gacggcgatc gcaatcgaca cgatcatcaa ccagcggcag atgtggggca ccgacaaggc
     7801 agtgaagtgc atctacgtcg ccgtcggcca gaaggcctcc acggtcgccg aggtcgtcgg
     7861 cgccctcgaa gaggccggcg cgatggagta cacggtcgtc gtcaacgcgg cggcgtccga
     7921 ccccgcgccg ttccagttcc tcgcccctta ctccggctgc gccatgggcc agcactggat
     7981 ggagaacggc gagcactcgc tgatcgtcta cgacgacctc agcaagcagg cgatcgcgta
     8041 ccggcagctc tcgctgctgc tccgccgccc gccgggccgc gaggcgtacc cgggcgacgt
     8101 cttctacctc cactcccgtc tgctcgagcg ttcggcgaag ctgtccgacg cgaacggcgg
     8161 cggctcgctg acggccttgc cgatcatcga gaccaagggc ggggacgtct cggcgtacat
     8221 cccgacgaac gtgatctcga tcaccgacgg gcagatcttc ctcgagtccg acctgttctt
     8281 ctcgggcgtc cgcccggcga tcaacgtcgg catctcggtc tcgcgagtcg gtgggaacgc
     8341 gcaggtcaag gctatgaagc aggtcgccgg ccgtctgcgt ctggacctcg cccagtaccg
     8401 cgagctcgag gcgttcgccg agttcggctc ggacctcgac aaggcgtcgc aggcccagct
     8461 cgaccgaggg gcccgcatcg tcgagatcct caagcagccg cagtacacgc cgttcccggt
     8521 cgaggaccag atcatctcga tctgggccgt cacgaacggg ttcgcggacg acgtcccgat
     8581 cgagaaggtg tcggccttcg aggccgcgct gctcgaccac gtgcgcaccc ggcactcgag
     8641 cctcgccgag tcgatccgca ccagcggcaa gctcgacgag acggccgagg agagcctcaa
     8701 gaaggccgtc ggcgagttca agtcgacctg gggtgacacg accgagacga cggcttcgac
     8761 cgcgggcgtg cccgacgccg gtggtggcgg tgccgtcaac gccgcgaccc cgggcgacac
     8821 gaccgcgcag ctcgacacgg ccggcacgga ctcggtctcg ggggagtagc cgatggctca
     8881 gctccgggac atcaagcgca ggatcgggtc ggtccaatcg acccagaaga tcacgcgcgc
     8941 catggagctc atcgcgtcgt cgcgcatcat caaggcgcag cagcgcgtgg acgcggcccg
     9001 gccctacgcc gaggagatgc gcagcctgat ggcctcggtc gccaagaacg ccgggagcat
     9061 cgaccacccg ctcctcaagc agcgcgacac cgtcggcacc gtcggcacga tcgtgatcac
     9121 cgcggaccgg ggcctcgccg gcgcgtacaa ctcgaacgtc atccgggcag ccgagaggga
     9181 catgcgggac cacgagcaga cacgtctgtt cgtcgccggg aagaagggcg tctcgtactt
     9241 ccgcttccgc ggctacgacc tcaccgagtc ctggcagggg atgtccgacc agccgaggat
     9301 cgacgacgca cgggcggttg cgaagtccgc tgcggacgcg ttcacgaacg gtgacgtcga
     9361 cgaggtccgt ctcgcctaca cgaagttcga gtcggcgctg acgcagcgtc cgacggtcct
     9421 gaagctgttg ccggtgccgc gcgccgacct ggacgagagc cagtcgagcg ccgacgggcc
     9481 gaagccgagc tacgtgttcg agccggcccc cgaggacatc ctcggctacc tgctcccgcg
     9541 ctacatcgaa ggtgcggtgt accaggggat gctcgaggcc gcggcttccg agcacgcggc
     9601 gcgccgccgt gcgatgaagg cggcgacgga caacgcggac gagctcatcg agaacctcac
     9661 gcgggtctac aaccaggcac gccaggcaga gatcacgacg gagatcatgg aggtcgtcgg
     9721 cggcgcggaa gcgctctcgg cgggccgcgc gaacgactaa ggagacgaga tggcagtaac
     9781 cgaagagaga ctcagccagc cgaagaccag cgagggccgg gtcatgaccg tgaccggccc
     9841 ggtcatcgag gtcgagttcc cgccggacgc gctccccgag atcaactacg cgctcgaggt
     9901 cgagcggacg atcgcaggga agaccgacac gatcacggcc gaggtcagcc agcacatcgg
     9961 cgcctccgtc gtgaaggcga tctgcatgaa ggcgacggac ggcctcgtgc gcgggacgaa
    10021 ggtgctcaac accgggctcc cgatcacggt cccggtcggg ccgagcacgc tcggccacgt
    10081 ctacaacgtc ctcggcgagc cggtcgacga gtcgtcggtg cccgagggtg tcgagcggtg
    10141 gccgatccac aggcagccgc cgaagttcga ggacctcgag ccgaagaccg agatgttcga
    10201 gaccggcatc aaggtcatcg acctcctcga gccgtacgtg cagggtggga agatcgggat
    10261 gttcggcggt gccggcgtcg gcaagaccgt cgtcatccag gagatgatct accgcgtcgc
    10321 cgagcagcac ggtggtgtgt cggtgttcgc cggcgtcggc gagcgcacgc gtgagggcaa
    10381 cgacctctgg ctcgagatga aggagtccgg cgtcctcgag aagacggcgc tcgtctacgg
    10441 ccagatggac gagcctccgg gagtgcgtct gcgcgtcgcg ctgagcgctc tcacgatggc
    10501 cgagtacttc cgcgacgtcg agcaccagga tgtgctgctg ttcatcgaca acatcttccg
    10561 cttcgtccag gccggctccg aggtctcgac gctgctcggc cgcatgcctt cggccgtggg
    10621 ctaccagccg acgctgtcgg aggagatggg cgagctccag gagcgcatca cgtcgacgaa
    10681 gggccgctcg atcacgtcgc tccaggccat ctacgtcccc gccgacgaca tcacggaccc
    10741 tgcgccgcac acggcgttcg cgcacctcga cgccaccacg gtgctctcgc ggcagatcgc
    10801 gtcgctgggc atctacccgg ccgtcgaccc gctcgactcg acgtcgcgga tcctcgaccc
    10861 ccgttacgtc ggtgacgagc actacaacgt cgccctgacg gtccagagga tcctccagcg
    10921 ctacaaggac ctccaggaca tcatcgcgat cctcgggatc gacgagctct cggaagagga
    10981 caagatcatc gtgcagaggg cgcgcaagat ccagcgcttc ctcagccagc cgttcttcgt
    11041 cgccgagcag ttcaccggcc tccccggcaa gtacgtcccg gtcgccgaca cgatcgcctc
    11101 gttcaagggc atcgccgagg gcgagtacga ccacctgccc gagcaggcct tctacctcgt
    11161 cggcgggatc gaagaggccg aggcgaaggc gaaggagctg tcggagtaat gcaggtctcg
    11221 atcgtctccc ccgaagaaga ggtgtggcgc ggcgacgccg acatggtcgt cgcccgctcg
    11281 cccgagggtg agttcggcat catgcgcggc cacatcccgt tcctggccgc gctagtgccg
    11341 ggccgcgtag cggtcgtgac cgggaaccag cgtgaggagt tcttcgtgcc cgggggcttc
    11401 ctcgaagcct ccggcacgag ggacgactac cacgtgatca tcctggcgga ctcggccgag
    11461 aacctcgccg acatcgacgt cgccgaagcc cgccgcatag cggaagaggc ttcgcgccgg
    11521 cgtgacgagg acaccgacga gcgcgccgag gccgagatgc gcgtggcgct cgcccgggtc
    11581 gagatcgcgg agagccgcgg ccactagcgg atgacggacg cgcaagggga gctgaggaac
    11641 aggatcttct gctcctcgtg cggttcgtcg acggcccgcc gcaacgggct cgcgccgctg
    11701 gggtggcagg agatgccgga cgcgaacaac cggcggatcg cgctgtgccc cgaatgcgtt
    11761 cgtcgcaacc tgtggctgat agaggcccgg ctggacatag atcccggctc cgggttctag
    11821 ataagggaaa ggcccccgtc ctggacgggg gccttttgct tgtccgatcg gcgccttacc
    11881 gcgagcacac cttgacgtcg ggcacagggc tcggcgcgag cagcgcgatg acgacgcacg
    11941 cgcagccgac gacgccttcc acgtactgcg ggcccatgat ctggcacgcg tcgcggtcgg
    12001 cggtcgaagc gtgggcgggg acggccccgg ccagcgagag cgcgccgatc acgccggcga
    12061 cgacgcccgt cctgatcttg ttcatccggt tcctcctctg gtcgggtggc tagacggtga
    12121 ggcggtcgac tacgtaggcc gcgcagtccg tgacgtcccg gatgatgttc ggcgacccga
    12181 tgaagcaccc gttcaccgtc tcgacgacgt agaacgcgca gtcggtgacg tcgcggacga
    12241 tgttcggcga gccgaggaag cacccgtccg tcgcagccgc ccgtgcgggg accgccgttc
    12301 cggcgaggag cgtcgccgct gcgaacgccg tcaccgccgc cttgcga
//
LOCUS       DASQDO010000043         9318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_923771, whole genome shotgun sequence.
ACCESSION   DASQDO010000043 DASQDO010000000
VERSION     DASQDO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923771"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="VEV43_01405"
     CDS             <1..2107
                     /locus_tag="VEV43_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22204.1"
                     /translation="RAQTTNCAGAGEPEDFGPTDVSATSGNQSLSAGLNEKATITVLK
                     WPSPSFYDQVKYRTVSRDEPRLGALPNEGALLGLAWRTAGASKWQFDWLRQWPASQRW
                     ADDDGDVIVTTFRRRAVGLRVTVRDVVAPDDDVLYRQVGVTRTARSKVRTVRVVGFAN
                     FNPVFTKTPQVPHQDWCSEEDNDSGASYDPDADAIVHARSGNDESTGSPSSVALTMGF
                     ASRSEGHSVGADTYEGASGTSAYDDAKDGKLSGEEGAPGQADAALLDQLSLTKRRSAT
                     STLLIAAAVTSEESITLLQDARKRTAPSVMRAKARWWRAWLEPAALPKNAPRAVGAVA
                     KRSLIAMRQATDPATRGPEEGGLIVASISTQPPLGLDWVRNGAYVNRALHEAGHDEMV
                     ARHDVRYAELQAATGRPPRGGTAATTAGNWAQNYYADGVVGGPVPYEIDSTGLGVWTL
                     WDHFAQTGDRFYLTQRGEVYEAIQRAAQYLTDICRDPTTSLHCASPDSQSTSPRVSLV
                     GAQAVWLGLGAAVGAANELGTPTAEANAAKWAARRVEIGAAIRDRFYDEDCNCYTDDP
                     GVGGTLLWPVGFLRYGSEQANAQAEVNFRHVSRAMRGRLDEGGDETKALLGNAFAWGG
                     SPEDLRRVRRGLAWVAQVPTSDGTHLLGERWVKRKGRVTTISGQPHVPTHAMFYVAAL
                     KAFGKERYSFRGRCSSGSR"
     gene            2086..3210
                     /locus_tag="VEV43_01410"
     CDS             2086..3210
                     /locus_tag="VEV43_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYP22205.1"
                     /translation="MLEWEQVTAWRIARGHLGRRTAASKLVEVVGRMCGAHAQIMSTV
                     PLALAARVNGVSAASVDSAIEQRTLVKTWAMRGTLHVFAADDVALYCAAQATRDQYQN
                     PSFLKYFGLEEADLHAVLDAIPQALDGRMLGRAELAREILRITKRKHLEERLLSGWGE
                     LLKPAAFRGRLCFGPGTGRNVTFVRPDQWIDDWKEYETDGALTEAFRRFLAAYGPASV
                     EEAARWWGVRPPEARRVFRLLGDELAEVEVGEAKRWMLARDVASVKRAKSPEGVRLLP
                     SFDQLLVMSAPHKVAVVDEPFFDRVYTPKPIAVWSLPAVLVDGRVRASWKLERKSKRA
                     LVKVAPFGKMTRAQRAALEAEAGRLGEVTGLAAELVLEEA"
     gene            complement(3207..4091)
                     /locus_tag="VEV43_01415"
     CDS             complement(3207..4091)
                     /locus_tag="VEV43_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP22206.1"
                     /translation="MTAVDQRASLVRSRLIERIERVDLRVRDVEPSLRFYRDVVGLEV
                     REQTPSRAVLASPGGPAFLALDSTGVTRPAEPRATGLFHVAIRFPHRASLGDALARLA
                     AAGLSIGAGDHAVSEALYISDPDGNGVELYWDRPVDEWPAPVPGALVPMVTLPVDLQD
                     LVSDGRGESAVGASAAAGTDMGHVHLQVSDVEQTARFYVDEVGLDLTARLGAEAAFLS
                     SNGYHHHLGANTWRSRGAKPASSESAGLERIVFAVDGRERVESLRLRLQEHGRTVSGD
                     EGAVVVRDPDGIELRFEA"
     gene            4187..5176
                     /locus_tag="VEV43_01420"
     CDS             4187..5176
                     /locus_tag="VEV43_01420"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HYP22207.1"
                     /translation="MQLSTKKRLILLSGDANRPLAREIADHLGMKLGEVEIATFANSE
                     NYTRIAESVRGWDAFVIQSIGDPVDHFIMQQLIMIDALKRASAKRITAVCPLYPYARQ
                     DRKAKGREPITAKLMADMYEAAGVDRMISVDLHSGQIQGFFDVPFDHLTAMPVLANHF
                     TEFLDDDFVIVSPDAGGVRLADKWVQHLADHHGLHGQVAFLHKRRSKDARNVSETKGV
                     VGDVAGKICVVVDDMIDTAGTLVDGSDLLVEAGATKVYAAATHGILSGPAIDRIKNSR
                     IEQVVVTNSLPLGPDKEIENIAVLSIAPLIAKTIQAVFEEGSVSTLFDGKNEP"
     gene            complement(5191..6252)
                     /locus_tag="VEV43_01425"
     CDS             complement(5191..6252)
                     /locus_tag="VEV43_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HYP22208.1"
                     /translation="MAANPVARQVFVGVDSGGTRTNVTIEVSNGAGPSVGDCYEVEEN
                     LSGALKPELVPDTLRTILAPLEIVADEHGLDGLDVYVWISAAGFTPWTRTHYLGAMSD
                     IAPGFLDGCIRSVGAANDGVSLLLGSRASGVVIAGTGSTVIVRSSDGKVHQAGGHEWV
                     ACDDGAGFWIGIRAIRQAYRDYESGTGSVLLQRFRQEYNVRARDDRAFIETLRELAIG
                     HKDMKKEIARFAARVCQAAERGDKSAQNIVKGEAESLADVTAEALRRHFASAELSRGL
                     SIVQCGSLLANEFYRKSFESQLEMRLRTDDRHAAQLTFERVATGAAAATQLAQDLAGS
                     TDEMLELDSAFRPAIVVAD"
     gene            complement(6234..6614)
                     /locus_tag="VEV43_01430"
     CDS             complement(6234..6614)
                     /locus_tag="VEV43_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22209.1"
                     /translation="MGEIVTVVVSVVIGLLTGWFFEHRATKAAERQNVQLRRDIIALK
                     TDVRSLGGDPDARRDPQPDSGLLEAVHARARATQDASGKVLRQELVAHFVQRGRAPDD
                     VEDAVAELCSSGAAKMEGRWLQIL"
     gene            complement(6701..7624)
                     /locus_tag="VEV43_01435"
     CDS             complement(6701..7624)
                     /locus_tag="VEV43_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYP22210.1"
                     /translation="MKSKLVPLIACLALTACGVFSDSAAGEGCCEPGAAQSSSAGPVP
                     TVSLPTGSPSEPPPFSFAVLGDFGTGDDVQRAVADRMCSWRDNHGFDLVFTTGDNVYP
                     DGHPSRFESAFYEPYECLTDDGVAFHAVLGNHDAQTDNGRPEIEDEAFGMDGRNYVVR
                     TNGVRFVMMDSNNVNMDFLRRATRAREGDLWTIVSMHHPVYSPGDGHGSEPGFRPKLP
                     RLFRKRGVDLVLQGHDHLYAVTKPLRGIRYVVTGGGGAGLYGCTSQPFSAKCEERHHF
                     LYVTATADALEVQAVPAKGKVFHSFTTEGID"
     gene            complement(7621..8271)
                     /locus_tag="VEV43_01440"
     CDS             complement(7621..8271)
                     /locus_tag="VEV43_01440"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYP22211.1"
                     /translation="MRRWYRRGAAVPRDNAGLARLIRRDLGEGPIARAFEEVDRGGFV
                     PPGSGDVYRDRPVPIPEHQTTSQPSLIARMIEAIDPRPGDRVLEVGTGLGYQTALLAK
                     LCREVVSLERWESLARLARSNLESAGTQNAEVIVGDGWKGCPERAPFDGIVVSAAATE
                     VPEPFEAQLADGGRLVIPVKRRGSDEVTLYVKKGGRLDEVRLVTPARFVPLVKEEH"
     gene            8270..>9318
                     /locus_tag="VEV43_01445"
     CDS             8270..>9318
                     /locus_tag="VEV43_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HYP22212.1"
                     /translation="MSRFERLSVLDELFLHLEGPDTHMHVGGAAIFEGPPPDYEEVLD
                     MVRGRLQLVPRFRQKLMTVPFGLGRPVWVDDTHFNLEYHVRHTALPSPGDDSKFRRLT
                     ARIMSQQLDRTKPLWEIWFAEGLTNDRFALISKTHHCLVDGVSGADIMSVILDLGPEP
                     QQVEPKAWRPDPEPTPDQLLLDALRERLTSPAEIVRTLQSSVIDPGKIPNRLIESVRA
                     LASFVGGTLDFAPTSSLNQPIGPHRRFETVLVELAEIKRIKNALDGTVHDVVHGAAER
                     VLDPLDLGELDEDRLEAPVRPDRLVERRGRREVERPPDEGGERAHALDQAVPDLAGVD
                     DARLEGAHDLGRGREP"
BASE COUNT         1451 a         3287 c         3220 g         1360 t
ORIGIN      
        1 gcgcgcccag accacgaact gcgcgggcgc aggcgagccg gaggacttcg gcccgaccga
       61 cgtctcggcc accagcggga accagtcgct gtcggcgggg ctgaacgaga aggcgacgat
      121 cacggtcctc aaatggccgt cgccctcgtt ctacgaccag gtcaagtacc gcaccgtcag
      181 ccgggacgag ccgcggctag gggcgctacc gaacgagggc gcgctgctgg ggctcgcgtg
      241 gcggacggcc ggggcgtcga agtggcagtt cgactggctc cggcagtggc cggcgtcaca
      301 gaggtgggcc gacgacgacg gcgacgtgat cgtgacgact ttccggcgaa gggccgtcgg
      361 gctacgtgtc accgtgcgcg acgtcgtcgc cccggacgac gacgtcctct accggcaggt
      421 cggcgtcacg aggacggcgc gctcgaaggt gcgcacggtg cgcgtcgtcg ggttcgccaa
      481 cttcaacccg gtcttcacga agacgccgca ggtccctcac caggactggt gctccgagga
      541 ggacaacgac tccggcgcgt cctacgaccc cgacgccgac gcgatcgtcc acgcgcgctc
      601 cggaaacgac gagtccacgg gctccccctc ttcggtcgcg ctgacgatgg gattcgcaag
      661 ccgctccgaa ggtcactcgg taggagccga cacgtacgag ggcgcgtcgg gcaccagcgc
      721 ctacgacgac gcgaaagacg ggaagctgtc gggcgaggag ggggcgccgg gccaggccga
      781 cgccgcgctc ctcgaccagc tctcgctcac caagcggcgg agtgcgacgt cgaccttgct
      841 gatcgccgcg gcggtcacgt ccgaagagtc gatcacgctc ctgcaggacg cacgcaaacg
      901 caccgccccc tcggtcatga gggccaaggc gcgctggtgg agggcgtggc tcgagcccgc
      961 ggcgctgccc aagaacgctc cgcgcgcggt cggcgcggtc gccaagcgat cgctgatcgc
     1021 gatgaggcag gcgaccgacc ccgcgacgcg agggcccgag gaaggcggcc tgatcgtggc
     1081 ctcgatctct acgcagcccc cgctcggcct cgactgggtg cgcaacggcg cctacgtcaa
     1141 ccgcgctctg cacgaggcgg gccacgacga gatggtcgcg cgtcacgacg tccgctacgc
     1201 ggagctgcag gccgcgaccg gccggccccc gcgcggcggc accgccgcga caaccgcggg
     1261 gaactgggcg cagaactact acgcagacgg agtcgtcgga gggccggtgc cgtacgagat
     1321 cgactcgacg gggctcggcg tatggacgct gtgggaccac ttcgcgcaga ccggcgaccg
     1381 cttctacctg acccagcgcg gcgaggtgta cgaggcgatc cagcgcgccg cgcagtacct
     1441 gaccgacatc tgccgcgacc cgacgacgag cctccactgc gcgtcaccgg acagccagag
     1501 cacgagcccg cgggtgtctc tcgtcggcgc tcaggcagtg tggctcggcc tcggcgcggc
     1561 ggtcggcgcg gcgaacgagc tgggcacgcc gacggcggag gcgaacgctg cgaagtgggc
     1621 cgcgcgccgc gtcgagatcg gcgccgctat ccgggaccgc ttctacgacg aggactgcaa
     1681 ctgctacacc gacgaccccg gcgtcggcgg gacgctgctg tggccggtgg ggttccttcg
     1741 ttacgggtcg gagcaggcga acgcgcaggc cgaggtcaac ttccgccacg tgtcgcgcgc
     1801 gatgcgcggc cggctcgacg agggcggcga cgagacgaag gccctgctcg gcaacgcgtt
     1861 cgcgtgggga ggcagcccgg aggacctccg gagggtccgg cgcgggctcg catgggtcgc
     1921 gcaggtgccc acctcggacg ggacgcacct cctcggcgag cgatgggtca agcggaaggg
     1981 tcgcgtgacg acgatctccg gccagcctca cgtgcctacg cacgcgatgt tctacgtagc
     2041 cgcgctgaag gcgttcggca aggagcgcta ctccttccgt ggacggtgct cgagtgggag
     2101 caggtgacgg cgtggaggat cgcgcggggg cacctcgggc ggcgcactgc cgcgagcaag
     2161 ctcgtcgagg tcgtcggccg catgtgcggc gcgcacgcgc agatcatgtc gaccgtcccg
     2221 ctggcgctcg ccgcgcgcgt caacggcgtg agcgccgcat ccgtcgactc cgcgatcgag
     2281 caacgcacgc tcgtcaagac gtgggcgatg cgcggcacgc tccacgtctt cgccgcggat
     2341 gacgtcgcgc tctactgcgc cgctcaagcg acccgcgacc agtaccagaa cccttccttc
     2401 ctcaagtact tcgggctcga ggaggcagac ctgcacgccg tcctcgacgc gatcccgcag
     2461 gcgctcgacg gccgcatgct cggccgggcg gagctcgcgc gcgagatcct gcgcatcacc
     2521 aagcgcaagc acctggagga gcgcctgctc tccggctggg gcgagctgct gaagcccgcc
     2581 gcgttccggg ggcggctgtg cttcgggccc ggcaccggcc gcaacgtgac gttcgtgcga
     2641 cccgaccagt ggatcgacga ctggaaggag tacgagaccg acggagcgct caccgaggcg
     2701 ttccgcaggt tcctggccgc gtacgggcct gcgtcggtag aggaggcggc gcgctggtgg
     2761 ggcgtgcggc cccccgaggc gcggcgggtg ttccgcctgc tcggtgacga gctcgcggag
     2821 gtcgaggtcg gtgaggcgaa gcgctggatg ctcgcccgcg acgtggcgtc ggtgaagcgc
     2881 gccaagtcgc ccgaaggggt ccgtctgctg ccgtcgttcg accagctgct ggtcatgagc
     2941 gcgccgcaca aggtcgcggt cgtcgacgag ccgttcttcg accgcgtcta cacgccgaag
     3001 ccgatcgcgg tgtggtcgct tcccgcggtc ctggtcgacg gaagggtgcg cgcctcgtgg
     3061 aagttagagc gcaagtcgaa gcgagcgctc gtcaaggtcg cgccgttcgg caagatgacg
     3121 cgcgcgcaga gggccgcgct cgaagccgag gccggccggc tcggcgaagt gacgggcctg
     3181 gcggcggagc tggtgctcga agaggcctag gcctcgaagc ggagctcgat cccgtcgggg
     3241 tcccgcacga cgacggcccc ctcgtctccc gagaccgtgc ggccgtgctc ttgaagtctc
     3301 agcctgaggc tctcgacgcg ctcgcgtccg tcgaccgcga agacgatccg ctcgagcccg
     3361 gcggactcgc tcgacgccgg cttcgcgccg cgtgaacgcc acgtgttcgc gccgaggtgg
     3421 tggtggtagc cgttcgaaga aaggaaagca gcctcggccc ccaggcgcgc ggtcaggtcg
     3481 agccccacct cgtccacgta gaaccgcgcc gtctgctcga cgtcggagac ctggaggtgc
     3541 acgtggccca tgtcggtccc ggccgccgcc gaagcgccga ccgcggactc gccccgtccg
     3601 tcggacacca ggtcctgcag gtcgacgggc agcgtcacca tcggcacgag tgcacccggc
     3661 acgggggccg gccactcgtc cacgggccgg tcccagtaga gctcgacgcc gttgccgtcg
     3721 gggtcactga tgtagagcgc ttcgctcacc gcatggtcac cggctccgat ggacagaccg
     3781 gcggccgcga gccgcgccag cgcgtcaccg agggacgccc tgtgggggaa gcggatcgcg
     3841 acgtggaaca gcccggtcgc acggggttcc gccggccggg tgacgcccgt cgagtcgagc
     3901 gcgaggaaag ccggcccgcc cggggacgcg aggacggccc ggctcggcgt ctgctcgcgc
     3961 acctcgagac cgaccacgtc cctgtagaac cgcagggacg gctcgacgtc gcggacacgc
     4021 aggtcgacgc gctcgatccg ctcgatgagc cggcttcgaa ccagcgaagc ccgttggtcc
     4081 accgcagtca tcggaacctc ctcctcgggt cgcggaacgc ccgcacctct ccagcgggaa
     4141 cgacgccccg gcccccggca ttccggcgcg gatacactcg ctccccatgc agctgtcgac
     4201 caagaaacgg ttgatcctcc tgtccggcga cgccaaccgt ccgctcgcga gggaaatcgc
     4261 ggaccatctg gggatgaagc tcggcgaggt cgagatcgcg acgttcgcca actccgagaa
     4321 ctacacgcgc atcgccgagt ccgtccgggg ttgggacgcc ttcgtgatcc agagcatcgg
     4381 cgaccccgtc gaccacttca tcatgcagca gctgatcatg atcgatgccc tgaagcgggc
     4441 ctcggcgaag cggatcaccg ccgtctgccc gctctacccg tacgcccgcc aggatcgcaa
     4501 ggcgaagggc cgcgagccga tcacggcgaa gctgatggcc gacatgtacg aggcggccgg
     4561 cgtcgaccgc atgatctcgg tcgacctcca ctcggggcag atccagggtt tcttcgacgt
     4621 cccgttcgac cacctcacgg cgatgccggt gttggccaat cacttcaccg agttcctcga
     4681 cgacgacttc gtcatcgtgt cgcccgacgc cggcggcgtg cggctcgccg acaagtgggt
     4741 ccagcacctc gcggaccatc acggcctgca cggccaggtc gcgttcctgc acaagcggcg
     4801 gagcaaggac gcccgcaacg tgagcgagac gaagggcgtg gtcggtgacg tcgcaggcaa
     4861 gatctgcgtg gtcgtcgacg acatgatcga caccgcgggg acgctcgtgg acgggtcgga
     4921 cctgctggtc gaggcgggcg ccacgaaggt gtacgccgcg gcgacgcacg ggatcctgtc
     4981 cggccccgcg atcgaccgga tcaagaactc gcggatcgag caggtcgtgg tgacgaactc
     5041 gctcccgctg gggccggaca aggagatcga gaacatcgcc gtgctgtcga tcgcgccgct
     5101 gatcgcgaag acgatccagg cggtgttcga ggagggctcg gtgtcgacgc tgttcgacgg
     5161 gaagaacgag ccctaggggg ccgagcggtc ctagtcggcg acgacgatcg ccggtctgaa
     5221 agcgctgtcg agctcgagca tctcgtccgt cgacccggcc aggtcctgtg cgagttgagt
     5281 tgccgcggcg gcgccggtgg ccactcgctc gaacgtcagc tgcgccgcat gccggtcgtc
     5341 ggtgcggagc cgcatctcga gctggctctc gaagctcttg cgatagaact cgttggcgag
     5401 caggctcccg cactggacga tgctcagccc ccgcgacagc tcggcggatg cgaagtggcg
     5461 cctcagagct tcagcggtca cgtccgcgag cgactcggcc tcgcccttca cgatgttctg
     5521 cgcgctcttg tcgcccctct ccgctgcctg gcagacccgc gccgcgaagc gggcgatctc
     5581 cttcttcatg tccttgtggc cgatcgccag ctctcgaagc gtctcgatga acgcgcggtc
     5641 gtccctcgcg cgaacgttgt actcctggcg gaaccgctgc aacagaaccg agcccgtgcc
     5701 agactcatag tcgcgatagg cctgacggat cgccctgatc ccgatccaga accccgcgcc
     5761 gtcgtcgcac gccacccact cgtgccctcc cgcttggtgc accttcccgt cggaggagcg
     5821 gacgatgacg gtggaacccg tcccggcgat gacgacgccg ctggccctcg accccagcag
     5881 aagggacacg ccgtcgttgg cggcacccac gcttcggatg catccgtcga ggaacccggg
     5941 agcgatgtcg ctcatcgcgc cgaggtagtg cgtacgggtc cacggcgtga agccggcggc
     6001 gctgatccag acgtacacgt ccagaccgtc gaggccgtgc tcgtcggcga cgatctcgag
     6061 tggcgccagg atcgtcctca gcgtgtccgg gacgagctcg ggcttgagcg ctcccgacag
     6121 gttctcctcg acctcgtagc agtcgccgac cgaggggccc gcgccgttcg agacctcgat
     6181 cgtcacgttc gtccgcgtcc ctccggaatc gacgccgacg aacacttgcc gcgctacagg
     6241 atttgcagcc atcgcccctc catcttggcg gccccagagc tgcacagctc cgcaaccgcg
     6301 tcctcgacgt cgtccggggc gcggccgcgc tggacgaagt gggcgacgag ctcctggcga
     6361 aggaccttcc cgctcgcgtc ctgagtggcg cgcgcccggg cgtgcacggc ttcgagcagg
     6421 ccggaatcgg gttggggatc gcggcgagcg tcggggtctc cgccgaggct gcggacgtcc
     6481 gtcttcagcg caatgatgtc tcgacgcagc tggacgttct gtcgctcagc agctttggtg
     6541 gccctgtgct cgaagaacca tcccgtcagg agaccgatca ccacactgac gactaccgta
     6601 acgatctcgc ccaccacagc ccagaccctt caaagcccac ttccccagtt gtcgaatcct
     6661 acgttaaaag cggccgtcgg cgccctcgag ccctagcggc ctagtcgatg ccctcggtcg
     6721 tgaaggagtg gaagaccttg cccttcgccg ggacggcctg gacctccagc gcgtcggccg
     6781 tcgccgtcac gtacaggaag tggtgccgtt cctcgcactt cgcgctgaag ggctggctcg
     6841 tgcagccgta cagcccggcc cccccgccgc cggtcaccac gtagcggatc ccgcgcaacg
     6901 gcttcgtcac cgcgtagagg tggtcgtggc cttggaggac gaggtcgaca ccgcgcttgc
     6961 ggaacaggcg cgggagcttc ggacggaagc ccggctcgga gccgtgcccg tcgccgggcg
     7021 agtagaccgg gtggtgcatc gacacgatcg tccagaggtc gccttcccgg gcgcgcgtcg
     7081 cgcggcgcag gaagtccatg ttcacgttgt tcgagtccat catcacgaag cgcacgccgt
     7141 tggtccgcac gacgtagttg cgcccgtcca tgccgaacgc ctcgtcttcg atctccggac
     7201 ggccgttgtc ggtctgcgcg tcgtggttgc caagcaccgc gtggaacgcg acgccgtcgt
     7261 cggtgaggca ctcgtagggc tcgtagaacg cggactcgaa gcggctcggg tgcccgtccg
     7321 ggtacacgtt gtcgccggtc gtgaagacga ggtcgaagcc gtggttgtcg cgccacgagc
     7381 acatgcggtc ggcgaccgct cgctggacgt cgtcgccggt gccgaagtcg ccgaggaccg
     7441 cgaacgagaa cggcggcggc tcggacgggg accccgtggg caacgagacc gtcgggacgg
     7501 gcccggccga gctcgactgc gcggcccccg gctcgcagca gccttcgccg gcggcggaat
     7561 ccgagaacac cccgcacgcc gtcagcgcga ggcaggcgat cagcgggacc agcttggact
     7621 tcaatgctcc tccttcacga gcggcacgaa cctcgccggc gtcacgagac ggacctcgtc
     7681 gaggcggcca cccttcttca cgtacagcgt gacctcgtcg ctgccacgcc tcttcaccgg
     7741 gatcacgagc cggccgccgt cggcgagctg cgcctcgaac ggctcgggca cctccgtcgc
     7801 ggccgccgac acgacgatcc cgtcgaacgg cgcccgctcc ggacacccct tccatccgtc
     7861 accgactatc acctcggcgt tttgggtccc tgctgattcg agattcgagc gcgcgagccg
     7921 cgccaggctc tcccagcgct cgagtgagac gacctcgcgg cacagcttcg cgagcagcgc
     7981 cgtctggtag cccagcccgg tcccgacctc cagcacccgg tcgccgggac gggggtcgat
     8041 cgcctcgatc atccgggcga tcagcgaggg ctgcgacgtc gtctggtgct cggggatcgg
     8101 taccgggcgg tcccggtaga cgtcgccgga gcccggaggg acgaagccgc cgcgatcgac
     8161 ctcttcgaag gctctcgcga tcggcccttc tccgaggtcg cggcggatca gccgggcgag
     8221 gcccgcgtta tccctcggca cggccgcgcc ccggcggtac catcgccgca tgtcgcgctt
     8281 cgaacgcctg agcgtcctcg acgagctgtt cctgcatctc gaaggacccg atacccacat
     8341 gcacgtcgga ggagccgcga tcttcgaagg tcctccgccc gactacgaag aggtcctcga
     8401 catggttcgg gggcggctgc agttggtgcc gcgcttccgt cagaagctca tgacggtgcc
     8461 gttcgggctc gggcggccgg tgtgggtcga cgacacgcac ttcaacctcg agtaccacgt
     8521 ccgccacacc gcgctcccct cccccggcga cgactcgaag ttccgccggc tcaccgctcg
     8581 catcatgtcg cagcagctcg accggacgaa gccgctgtgg gagatctggt tcgccgaggg
     8641 cctcacgaac gaccgtttcg cgctcatcag caagacccat cactgtctgg tcgacggggt
     8701 ctcgggagcc gacatcatgt cggtcatcct cgacctcggg cccgagcctc agcaagtcga
     8761 accgaaggcg tggcggcccg accccgagcc gacgccggac cagctcttgc tcgacgcgtt
     8821 gcgcgagcgg ctcacgtccc ccgccgagat cgtgcggacc ctccagtcga gcgtcatcga
     8881 ccccggcaag atcccgaacc gcctgatcga gagcgtgcgc gctctcgcct cgttcgtcgg
     8941 cgggacgctc gacttcgcac cgacctcgtc gctcaaccag ccgatcggcc cgcaccggcg
     9001 gttcgagacc gtgctcgtcg agctcgccga gatcaagagg atcaagaacg cgctcgacgg
     9061 cacggtccac gacgtcgttc acggtgccgc cgagcgcgtt cttgatcctc ttgatctcgg
     9121 cgagctcgac gaggaccgtc tcgaagcgcc ggtgcggccc gatcggctgg ttgagcgacg
     9181 aggtcggcgc gaagtcgagc gtccccccga cgaaggaggc gagcgcgcgc acgctctcga
     9241 tcaggcggtt ccggatcttg ccggggtcga tgacgctcga ctggagggtg cgcacgatct
     9301 cggccgggga cgtgagcc
//
LOCUS       DASQDO010000044         6766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1046138, whole genome shotgun sequence.
ACCESSION   DASQDO010000044 DASQDO010000000
VERSION     DASQDO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046138"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="sat"
                     /locus_tag="VEV43_01450"
     CDS             complement(<1..1035)
                     /gene="sat"
                     /locus_tag="VEV43_01450"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HYP22213.1"
                     /translation="MPSTLKDVPGSSAPHGGALELRIAGDEEKAALVAEAANLPKIEA
                     KSRRVLSDLELLAIGAVSPNRGFMTEDDYKSVVEEMRLANGLAWSLPITLSATDEEVA
                     ALSKGTRAAIVHEGKPVAIIEVEDVYTYDAKREAELTYKTTDDAHPGVAYLYSQAPNY
                     VGGEVTVLENVFAHEFSEYRLTPLALREKIAERGWKTVVAFQTRNPIHRAHEYLTKCA
                     LEITDGLVIHPLVGGTKGDDIPAEVRMQCYGVLMDNYYPQDRVVLSVFPAAMRYAGPK
                     EAIFHALVRKNYGCTHFIVGRDHAGVGTYYGTYDAQQIFEEFEPGELGITTLNFEHSF
                     WCEACEGMASP"
     gene            complement(1179..1919)
                     /locus_tag="VEV43_01455"
     CDS             complement(1179..1919)
                     /locus_tag="VEV43_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22214.1"
                     /translation="MADVDLVQLEGAIKRLPDVLGCVILTDPQGRAGEIQAFTRSGSD
                     KNTVHQGILDTAAQVGVGDELRQVLVFELEAESIFGDRESLERAAELAEQEARARGIQ
                     WEPDPPLEHDPQTRDITGHAFRPPLRRVVLSSSAWKTHAEVVLGHDDGDEVRGESSGD
                     KTPHGLKVLAQATLDAVANLVGVADFMLEGASLVSVVGQEAVLVMLRLHDGLEVLGAA
                     LVRDGPVSEAAVRATLDGLNRRFARLAE"
     gene            2307..3419
                     /locus_tag="VEV43_01460"
     CDS             2307..3419
                     /locus_tag="VEV43_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYP22215.1"
                     /translation="MQGRLGRTSIVATLLCTLLIPAAGSLADPDNKLDEIERDLGHVR
                     ERIEATESDAAPLEEQVDVLSSQMISLRDQLARLDERVATVQAAVRSAQAQIDETQRK
                     IDKIKDRAVEQAVSLYKAGSTETIDALLNAESLSQLDARVEMLGVAAEKSTGALIQYG
                     RLRVTIEAQNRELFERKAELEATRKEKSSTYATLREKYTEHKAALMKLERKLGHLHAE
                     EGSLEQAALRLTQDLRANQASAAAAALGESSTGFMWPLNGAVTSYYGPRWGSMHTGLD
                     IDGTTGQPVVASKAGTVSMASSYSGYGNAVIVDHGGGYQTLYAHLSSFAVSAGQTVAQ
                     GQVVGNVGCTGSCTGDHLHFEVRVNGSPQDPMNYLP"
     gene            complement(3424..4902)
                     /locus_tag="VEV43_01465"
     CDS             complement(3424..4902)
                     /locus_tag="VEV43_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP22216.1"
                     /translation="MPRAVRFVALIAGLAVASAAAGAQASRPPRAWTCLGRPATVVGS
                     PGADRLVGTNGRDVIVGRGGNDEIVGHDGDDLLCGGDGRDRIEGRYGDDEIDAGAGND
                     VADGGPDGDLLVGGIGSDRLSGGDGGFGDTLVGGDGSDTLDGGDGRRDSDFLFGGASG
                     DFLDGGLGARDMLYGGSASDDFTGGIVSFELATGPAIVGLAAEPSGTSYAPGEDADDL
                     LSDIGGIVGTPYSDDVSGSDAAETFRGLDGDDRIDAAGGDDDVDGGAGSDVLVGGAGR
                     DPLRYEDSPVGVTASLRDGTAGGDHLAGFEDLFGSFFDDTLTGDDGPNHVDGSFGTNT
                     IFAAGGDDYLAWAADGDAGDGRDTCAYSGNVSGCEATMEAVFEALPSVSDPVHREELE
                     RLGVVSGEAFGDPRRVLVGIRRMTPDGCAWWDETDSRFEPGACGNVRGAKLRAPEGEW
                     ALRVRRTLRPGAYLVAAQWADSSGRLHCEAVFAPSCVAFDVR"
     gene            complement(4918..6192)
                     /locus_tag="VEV43_01470"
     CDS             complement(4918..6192)
                     /locus_tag="VEV43_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22217.1"
                     /translation="MAGRKKIGWCAAALAAAAAVPAAASPVPEPPQLWVMNADGTDQR
                     VLVADTDWRPSPAWSPDGRSLAYTTSNGMIAIVDVDGQAPARAIATGDHAEWSPDGSL
                     VAFVNSPQGNGGPGTLAVVRPDGSGLREVAGMAAPGPVEWSPDGTKIAYHRCDDSPCR
                     HSLAVVAPDGTGDVVLAPSEFGSEPSWAPDSSAIAFSAGGDLWVVTLASTNVRNLTPG
                     GDFGMRPRWGPGGRIAYIGDLGRTYLVDPDGTDRVLVHEGGLSDWSPDGTRLLVSYRG
                     DILVTEPGTEGSPNLTNSESSEYGGLWSPDGARIAYHWERPSGPFPQRRERSISASLS
                     DGVVLGRVSVVGDGPRTCASEVPLLLQRFGRRGWSTLDRGETTRKGRFEFDVGRREGL
                     YRAKAPKTWARGLAFEPDECAPASSPPLRHRR"
     gene            complement(6331..>6766)
                     /locus_tag="VEV43_01475"
     CDS             complement(6331..>6766)
                     /locus_tag="VEV43_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HYP22218.1"
                     /translation="AVEEGIVPGGGATLVRCRENVEALELTGDEAAGARVVMEALSAP
                     LWWIAANAGMEGGVVVDHVRNEKPGHGLNAATGEYVDLLAAGIIDPARVTRSALENAA
                     SIAALLLTTEATIVDKPQEEDPMAHAGHDHGMGGMGGMGGMM"
BASE COUNT         1006 a         2413 c         2379 g          968 t
ORIGIN      
        1 cggcgacgcc atgccctcgc acgcctcaca ccagaaggag tgctcgaagt tcaaggtggt
       61 gatgccgagc tcgcccggct cgaactcctc gaagatctgt tgcgcgtcgt aggtgccgta
      121 gtaggtgccc acccccgcgt ggtcgcggcc gacgatgaag tgcgtgcacc cgtagttctt
      181 ccggacgagc gcgtggaaga tggcctcctt ggggccggcg taccgcatcg ccgccgggaa
      241 caccgagagg acgacacggt cctgcgggta gtagttgtcc atgaggaccc cgtagcactg
      301 catgcgcacc tcggccggga tgtcgtcgcc cttggtcccg ccgacgagcg ggtggatcac
      361 gaggccgtcg gtgatctcga gcgcgcactt cgtcaggtac tcgtgtgcgc ggtggatcgg
      421 gttacgggtc tggaacgcga cgacggtctt ccagccgcgc tcggcgatct tctcgcgcag
      481 cgccagcggg gtgaggcggt actcggagaa ctcgtgagcg aacacgttct cgaggacggt
      541 cacctcgccg ccgacgtagt tcggcgcctg cgagtagagg tacgcgacgc cggggtgcgc
      601 gtcgtcggtg gtcttgtagg tgagctcggc ctcgcgcttc gcgtcgtacg tgtagacgtc
      661 ctcgacctcg atgatcgcga ccggcttgcc ctcgtggacg atcgcggccc gcgtgccctt
      721 ggacagggcg gcgacttcct cgtccgtcgc cgacagcgtg atcgggaggc tccacgcgag
      781 cccgttcgcg agccgcatct cctcgaccac cgacttgtag tcgtcctcgg tcatgaagcc
      841 gcggttcggc gacaccgcgc cgatcgcgag cagctcgagg tccgagagca cgcggcggga
      901 cttcgcctcg atcttgggca ggttcgcggc ctcggccacg agcgcagcct tctcctcgtc
      961 gcctgcgatc cgtagctcga gcgcgccgcc gtgcggggcg gaagagccgg ggacgtcctt
     1021 cagggtgctg ggcatggctt ctcctttcgt ttctttgccg agacccagct tccgccgcgg
     1081 cgggaccggg tgcatgtcga agaggtcttg cgcggaggcg tgaacactcc tatagcactc
     1141 cacaataaca gggcagcgcc ctgcgggcca ggagtgggct attcggccag gcgggcgaag
     1201 cggcggttca ggccgtcgag cgtggcacgc acggcggcct cgctcacggg gccgtcccgg
     1261 accagggcgg cccccagcac ctcgaggccg tcgtgcagcc gcagcatcac gagcacggcc
     1321 tcctggccga cgacgctgac cagcgaggca ccttcgagca tgaagtcggc cacgccgacg
     1381 aggttcgcga ccgcgtcgag ggtcgcctgg gcgaggacct tgagcccgtg gggggtcttg
     1441 tccccgctcg actcgccgcg gacctcgtcg ccgtcgtcgt ggccgaggac cacctcggcg
     1501 tgggtcttcc aggcgctgga ggagaggacg acccggcgga gcgggggccg gaaagcgtgt
     1561 cccgttatgt cccgggtctg ggggtcgtgc tcgagcggcg ggtccggctc ccactggatc
     1621 ccccgggcac gggcctcctg ctcggcgagc tcggcggcac gctcgaggga ctcgcggtcg
     1681 ccgaagatcg actcggcctc gagctcgaac accaggacct ggcggagctc gtcgccgacg
     1741 cctacctgcg ccgccgtgtc gaggatcccc tggtgcacgg tgttcttgtc gctgccgctg
     1801 cgcgtgaagg cctggatctc gccggcacgc ccctgggggt cggtgaggat gacgcatccg
     1861 aggacgtcgg ggaggcgctt gatcgcgccc tcgagctgga ccaggtctac gtcagccatc
     1921 gccccgcagg atatagagaa ggacccccca cgcaaatgca cccgtcgagc cttcgtcccg
     1981 gccgaggccg gtttacgact caacgggtgc gttccagaac atacccgaac ccacgcttcg
     2041 taggcaagcc ctcacgctga gttttattcg cggcggcccg gcaggagatt ggttttccgg
     2101 cgactaacta cggggggacc gccgctgttg ctgggctcga accgctgtgg caggatgccc
     2161 ggcggcccct ggggaggggc gagcggcggt aggccccggc accacctgcc gggctgcacg
     2221 aagggtcttc gcggccctct gacgggagaa cgggacgaga ccgcgcccga acaagggctg
     2281 gcgtcccccg aacggaaagg aaacggatgc agggaaggct cggtcgcacc tcgatcgtcg
     2341 ccacgctcct gtgcacgctt ctgatccccg cggcgggctc gctcgccgac cccgacaaca
     2401 agctcgacga gatcgaacgc gacctcgggc acgtccgcga gcggatcgag gccacggaat
     2461 cggacgcggc accgctggag gagcaggtcg acgtcctgag ctcgcagatg atctccctgc
     2521 gcgatcagct cgcgcggctc gacgagcgcg tggccaccgt ccaagcagcc gtccggagcg
     2581 cgcaggccca gatcgacgag acccagcgca agatcgacaa gatcaaggac cgcgccgtcg
     2641 aacaggcggt gtcgctctac aaggccgggt cgaccgagac catcgacgcc ctcctcaacg
     2701 ccgagtcgct ctcccagctc gacgcgcgcg tcgagatgct cggcgtcgcc gccgagaaga
     2761 gcacgggcgc gctgatccag tacggacggc tccgcgtcac gatcgaggcc cagaaccgcg
     2821 agctgttcga gcggaaggcc gagctcgagg cgacccggaa ggagaagagc agcacatacg
     2881 cgaccctccg cgagaagtac accgagcaca aggccgcgct gatgaagctc gagcggaagc
     2941 tcggccacct ccacgcggag gaaggaagcc tcgagcaggc cgcgctcagg ttgacgcagg
     3001 acctccgggc gaaccaggcg agcgcggccg ctgcagctct cggcgagtcg tcgacgggtt
     3061 tcatgtggcc gttgaacggc gcggtgactt cgtactacgg cccccgctgg ggcagcatgc
     3121 acacgggcct ggacatcgac gggaccacgg ggcagccggt ggtcgcgtcg aaggcgggga
     3181 ccgtctcgat ggcgtcgtcg tactcgggct acgggaacgc cgtgatcgtc gaccacggtg
     3241 gcggctacca gacgctgtac gcccacctct cgtccttcgc ggtgtcggcc gggcaaacgg
     3301 tcgcgcaggg tcaggtcgtc ggcaacgtcg gctgcacggg atcgtgcacc ggcgaccatc
     3361 tgcacttcga ggtgcgcgtg aacgggtcgc cgcaagaccc gatgaactac ctgccgtagc
     3421 gcctcagcgc acgtcgaacg cgacgcacga cggtgcgaac accgcctcgc agtggagcct
     3481 ccccgaagag tccgcccact gggctgccac gaggtaggcg ccgggccgta gcgtccttcg
     3541 gacccgcaag gcccactccc cctcgggggc ccggagcttc gcgccgcgga cgttcccaca
     3601 ggcaccgggc tcgaacctcg agtcggtctc gtcccaccac gcgcacccgt ccggcgtcat
     3661 cctgcggatc ccgaccaaga cgcggcgggg atcaccgaag gcctcgccgc tcacgacccc
     3721 gagccgctct agctcctcgc ggtgcaccgg gtccgagacg gacgggagcg cctcgaagac
     3781 ggcttccatc gtggcctcgc agccggagac gttgcccgag tacgcgcagg tgtctctccc
     3841 gtcaccggcg tcgccgtcgg cggcccaggc caggtagtcg tcgccgcccg ccgcgaagat
     3901 cgtgttcgtg ccgaacgagc cgtcgacgtg gttcggcccg tcgtcgccgg tgagagtgtc
     3961 gtcgaagaag gacccgaaca ggtcttcgaa gcccgcgagg tggtcgccgc ccgcggttcc
     4021 gtcccggagg ctcgccgtca cgcctaccgg ggagtcctcg taacgaagcg ggtcgcgacc
     4081 cgcgcccccc acgagcacgt cggaaccggc gccgccgtcg acgtcgtcgt caccgcccgc
     4141 cgcgtcgatc cgatcgtccc cgtcgagccc gcggaaggtc tcggccgcgt cgctgccgga
     4201 gacgtcgtcg gagtaagggg tgccgacgat cccgccgatg tccgaaagga ggtcgtcggc
     4261 gtcttcgccc ggggcgtacg acgtgcccga gggctcggcg gcgaggccga cgatcgccgg
     4321 gccggtcgcg agctcgaacg agacgatccc accggtgaag tcgtcgcttg cgcttccgcc
     4381 gtacagcatg tcgcgcgccc cgagcccgcc gtcgaggaag tcgcccgacg cacctccgaa
     4441 gaggaagtcg gagtcgcggc gcccgtcgcc cccgtcgagc gtgtcggatc cgtcgccgcc
     4501 gaccagagtg tcgccgaagc cgccgtcccc accggagagc cggtcggacc cgatgcctcc
     4561 gacgagcaag tccccgtccg gccccccgtc cgcgacgtcg ttgccggcgc cggcgtcgat
     4621 ctcgtcgtcg ccgtagcggc cctcgatccg gtcacggccg tcgccgccac acaggaggtc
     4681 gtcgccgtcg tggccgacga tctcgtcgtt cccgccgcgt ccgacgatga cgtcgcggcc
     4741 gttggtgccc accagacggt cggcgccggg ggacccgacg acggtggcgg gacgtccgag
     4801 acaggtccag gcgcgcgggg gccgggacgc ctgggccccg gccgcagccg aagcgacggc
     4861 gaggccggcg atcagggcga cgaagcgaac ggctctgggc accacgggac ctcgcagcta
     4921 gcggcggtgg cggaggggtg gtgacgaggc gggggcacat tcgtccggct cgaaggccaa
     4981 gccccgagcc catgtcttcg gcgccttcgc ccggtacagg ccctcacgcc ggcccacgtc
     5041 gaactcgaac cgcccctttc gcgtcgtctc gccgcggtcg agcgtcgacc agccgcggcg
     5101 gccgaaccgt tgcaggagga gcgggacctc cgacgcgcac gtccgcgggc cgtcgccgac
     5161 gacgctgacc ctcccgagga cgaccccgtc gctcagcgag gcgctgatcg aacgctcgcg
     5221 ccgctggggg aagggcccgg agggacgctc ccagtggtag gcgatccggg cgccgtccgg
     5281 agaccagagg ccgccgtact ccgacgactc gctgttcgtg aggttcggcg agccctcggt
     5341 ccccggctcg gtgaccagga tgtcaccccg gtaggacacg agcagccgtg tcccgtccgg
     5401 tgaccagtcg gataaccctc cctcgtgcac gagcacgcgg tcggttccgt cggggtcgac
     5461 taggtaggtg cgcccgaggt ccccgatgta ggcgatccgc ccgccgggcc cccagcgggg
     5521 gcgcatcccg aagtctccgc ccggagtcag gttccgcacg ttcgtcgacg cgagcgtcac
     5581 cacccatagg tctccgcccg cgctgaacgc gatcgccgag gagtcggggg cccacgacgg
     5641 ttcgctgccg aactccgaag gtgcgaggac gacgtcgccc gtgccgtcgg gcgcgaccac
     5701 cgcgagcgag tgccggcagg gcgagtcgtc gcagcggtgg tacgcgatct tcgtcccgtc
     5761 gggcgaccac tcgacggggc cgggcgcggc catgccggcg acctccctca gccccgagcc
     5821 gtcggggcgt acgaccgcga gcgtccccgg tccgccgttt ccctgcgggc tgttcacgaa
     5881 cgcgacgagc gagccgtcgg gggaccactc cgcatggtcc cctgtcgcga tcgcccgcgc
     5941 gggggcttga ccgtccacgt cgacgatcgc gatcatcccg ttgctcgtcg tgtaggcgag
     6001 ggaacgtccg tcgggcgacc acgcggggct cggcctccag tcggtgtcgg cgacgaggac
     6061 gcgctgatcc gtgccgtcgg cgttcatgac ccacagctgc ggcggctcgg gcaccgggct
     6121 cgcggcggcg gggaccgccg cggcggccgc gagcgcggcc gcgcaccacc cgatcttctt
     6181 ccgtcccgcc atacgtcgat gaaaacgtac cgggaccccg atagttgcct accggtcgag
     6241 gaagagcccg cgtcaaacaa agctagtcgg acgcgcaagg ggcccggcga cttcacgccg
     6301 ggcccctctt cgcgtccttg ggaggacggc ttacatcatg ccgcccatgc cccccatgcc
     6361 gcccatgccg tggtcgtgac cggcgtgagc catggggtct tcctcttgcg gcttgtcgac
     6421 gatcgtcgcc tccgtcgtaa gcaggagagc tgcgatcgac gcggcgttct cgagggcgga
     6481 gcgcgtcacg cgcgccgggt cgatgatccc ggcggcgagc aggtcgacgt actcgccggt
     6541 cgcggcgttg aggccgtgac ccggcttctc gttgcggacg tggtcgacga cgacgccacc
     6601 ctccatgccg gcgttcgcgg cgatccacca cagcggcgcg ctgagcgcct ccatgaccac
     6661 gcgcgcacca gcggcctcgt cacccgtcag ctcgagcgcc tcgacgttct cgcggcaacg
     6721 aacgagcgtc gcgccgccgc cggggacgat gccctcctcg acggcc
//
LOCUS       DASQDO010000045         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_250983, whole genome shotgun sequence.
ACCESSION   DASQDO010000045 DASQDO010000000
VERSION     DASQDO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250983"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VEV43_01480"
     CDS             complement(<1..1022)
                     /locus_tag="VEV43_01480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYP22219.1"
                     /translation="MSERAVVVGGGIVGSCVAWFLAEAGVDVVVVEKDPTNDRSSTSR
                     SAAAIRQQFHLGVNVAMSRFGYDFYAALEDVGFVERGYLVLATHDGLARLETAHRNQL
                     ANGASVDLLDAGELRRRVPWLKGDDVAAATFGTAGEGWFDPVAALAEIRAGAARRGAE
                     LVEDQVTGIELDGGRVARVTLRDGAPIACDHVVNAAGPAAGAVSAYAGYAIPVEPRKR
                     TVCVFRAGAHIDGFPNLVDPTVAGRGLYVRPLGDLYMAVTAPPPERDPATDDLEPDTY
                     LFADVIRDALARRVDGFEDAELVRAWAGHYEMNTFDQNATVGPHPDVEGFWFACGFSG
                     HGVMHAP"
     gene            complement(1019..1189)
                     /locus_tag="VEV43_01485"
     CDS             complement(1019..1189)
                     /locus_tag="VEV43_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22220.1"
                     /translation="MRGPARERLVDRGPGGVAQAKSATRSAATAIVFLMGAEVCPIRV
                     ELALLGTGKIDG"
     gene            1199..1330
                     /locus_tag="VEV43_01490"
     CDS             1199..1330
                     /locus_tag="VEV43_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22221.1"
                     /translation="MGCFEEGQRFLCGYCYEGGALNVVVAHSDAHGHIVVDVFYVAD"
     gene            complement(1351..2613)
                     /locus_tag="VEV43_01495"
     CDS             complement(1351..2613)
                     /locus_tag="VEV43_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22222.1"
                     /translation="MHDEQLSIAIKDAGEAFEPPWADVRALSRRGRRRWWLTRVGAAT
                     TSLALAAGVAVATSRIDLDGGEKPPVRAAAPGTLRHWEALPAAPIAGRSGAAVAWTGS
                     ELLVVAGSADRENFDDGASFDPATDSWEKLPTGPSSEIHGAAVVWTGRELIFWGGEDG
                     DDQPHRGAAYDPATRRWRTLPPAPSWSLGSHSAVWTGTEMLVWGGVWPGGPGPRGVAA
                     AYDPATNEWEAFPLGPLGRRHGHAAVWTGEEMVVWGGGSDDVAPPRGPQAAAYDPETR
                     TWRALPDSPLEPASSRVAFWTGKEVVLVGGLGAGPARDGAAYDPAADRWRSIAPVPLA
                     AQRDDQPIMLSDFSTRPAWTGRKAVFVTIDGVLSYDPATDRWTRERAPQDVSRHGAAV
                     AWTGDELLVWSGSHLGAKGYLETGWSGR"
     gene            complement(2606..3127)
                     /locus_tag="VEV43_01500"
     CDS             complement(2606..3127)
                     /locus_tag="VEV43_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP22223.1"
                     /translation="MNNRKRFPLFRTGPIGQGDELDFEEFFRGEYRTVFRAACLATGD
                     ADVASDATQEAFGRAYARWSRLAKHPWAGGWVTTTALNLCRKELRRRGRPAPRLESEA
                     GPGRDVPREVDTVAALGRLPFRQREAVVLFYWNDLPVAAIADLMAISESAVRAHLTHA
                     RTALRKTLEVNHA"
     gene            3199..4452
                     /locus_tag="VEV43_01505"
     CDS             3199..4452
                     /locus_tag="VEV43_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP22224.1"
                     /translation="MAATLARTFESLGVKNYRMFFTGQVVSWTGTWVQWIAQGWLVLR
                     LTQSGLGLGLVTALQWLPILLLGAWAGVVADRLDKRKLLIFTNVSSGVLSLVLGVLTV
                     AGVVTLWMVIAVALLLGVVTALDNPARQTFTMEMVGRAKLTNAVSLNTATFTTARVLG
                     PAIAGVTISTVGIGPCFLLNAISFVPVTLALIAMDRSQLRPSEAVARSKGQVREGLRY
                     VRSVPVLKTLLVMMAVIGTLQYNFAVLLPVLAKDTFAGDAGTLGLIGAAIGVGMFAGS
                     MTNAAFGRSDRRVLLAAGFALGTFTVLTAAAPSLWSAVLLMLPLGAASMAFLATMNST
                     LQLTSSDEMRGRVMAIYFVLFLGSTPIGAPIVGWLSEQFDPRVALGFGGVATLLACVY
                     GLLRLPPLDTRPATEDPEEELVVAS"
     gene            4552..5256
                     /locus_tag="VEV43_01510"
     CDS             4552..5256
                     /locus_tag="VEV43_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22225.1"
                     /translation="MKKLVGACVVALLVGAMALPAGAKAAKPTVAFEDAAGDAGVQNS
                     STPGAAEAGFDLIKGEISQDKKGGDVKFVVSHASMPPTSQPAEAFRFIWGFAVDTEQY
                     EFTVKSIDVGEPDVITSGITQDPHGEERVGQVYQGVARLETCGSVAAPSLTLATCDAQ
                     EYFTATFDPAAGTVTWSVPLASIGAKKGSLITGGGGTRTATGCQICWVPHYLERSLTP
                     HTIVDAAAQLKSYKVK"
     gene            5372..5584
                     /locus_tag="VEV43_01515"
     CDS             5372..5584
                     /locus_tag="VEV43_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22226.1"
                     /translation="MRKKLVAPALIATALAVWPASPAGATHNPCIPSDQVCIRIQCPN
                     PSHGAFYIYIDSRGLPFVDFTDCAVA"
     gene            complement(5581..>6041)
                     /locus_tag="VEV43_01520"
     CDS             complement(5581..>6041)
                     /locus_tag="VEV43_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22227.1"
                     /translation="KMRRLARQERFEEAAELRDRARSLARWIERSIEARALIDAGEVL
                     LLVGGRAILLVEGQLASACDAGDRTVDAVLASLRKTATWEPVGEWLTAAVQREVRAVV
                     SWLNRHGGEVGVLHVGGPWSVPARAKPTEAFAKVERGPRGGRVLQTGPSA"
BASE COUNT          823 a         2225 c         2090 g          903 t
ORIGIN      
        1 ggcgcgtgca tgacgccgtg cccgctgaag ccgcacgcga accagaagcc ctccacgtcc
       61 gggtgcggcc cgacggtcgc gttctggtcg aacgtgttca tctcgtagtg cccggcccac
      121 gcgcgcacga gctccgcgtc ctcgaagccg tcgacgcggc gcgccagcgc gtcgcggatc
      181 acgtcggcga acaggtacgt gtccggttcg aggtcgtcgg tcgcgggatc gcgttcgggc
      241 ggcggcgcgg tcacggccat gtagaggtcg cccagcggac ggacgtagag gccccgccct
      301 gcgacggtgg gatcgaccag gttggggaac ccgtcgatgt gtgctccggc gcggaacacg
      361 cagacggtgc gcttgcgggg ctcgaccggg atcgcgtagc cggcgtacgc cgacaccgcg
      421 ccggccgcag gccccgcagc gttcaccacg tggtcgcacg cgatcggtgc gccgtcccgc
      481 agcgtcacgc gcgcgacccg gcccccgtcc agctcgatcc ccgtgacctg gtcttcgaca
      541 agctcggccc cgcggcgggc ggcgccggcg cggatctccg ccagggcggc gacggggtcg
      601 aaccagcctt cgcccgcggt gccgaacgtc gcggccgcga cgtcgtctcc cttcagccac
      661 gggacccgcc ggcgtagctc gcccgcgtcg aggaggtcga cgctcgcgcc gttcgcgagc
      721 tggttgcggt gcgcggtctc gaggcgcgcc agcccgtcgt gtgtcgcgag gaccaggtag
      781 ccgcgctcga cgaacccgac gtcttcgagg gccgcgtaga agtcgtagcc gaagcgcgac
      841 atcgcgacgt tgacgccgag gtggaactgc tggcggatcg cggccgccga ccggctcgtc
      901 gaggaccggt cgttggtcgg gtccttctcc acgacgacga cgtcgacccc ggcctcggcg
      961 aggaaccacg cgacgcagga cccgacgatc ccgcccccga cgacgaccgc tcgctcgctc
     1021 atccgtcgat cttgcccgtc ccaagcaatg caagctcgac acggatcggg cacacctccg
     1081 cccccatgag gaagacgatc gcagtcgccg cgctcctcgt cgcgctcttc gcctgcgcta
     1141 cgccacccgg gccgcgatcg acgaggcgtt ctcgggccgg cccccgcacg ccgagttcat
     1201 ggggtgcttc gaggaggggc agcggttcct gtgcggctac tgctacgagg gcggagcgct
     1261 caacgtcgta gtggcccact cggacgcgca cggccacatc gtcgtcgacg tcttctacgt
     1321 ggccgactga ttcggcgtgc cctggtccgg ctagcggccg ctccacccgg tctcgaggta
     1381 gcccttcgcg cccaggtgcg agccgctcca cacgagtagc tcgtcgcccg tccatgcgac
     1441 ggccgccccg tggcgcgaca cgtcctgcgg cgcacgttcc cgcgtccacc ggtcggtcgc
     1501 ggggtcgtag gacagcacgc cgtcgatggt gacgaagacc gccttgcgcc cggtccacgc
     1561 cggcctcgtc gagaagtcgc tcagcatgat cggctggtcg tccctctggg ccgccagcgg
     1621 gaccggcgcg atcgaccgcc agcggtccgc ggcggggtcg taggccgcgc cgtcacgcgc
     1681 cggcccggcc ccgagcccgc ccacgaggac gacctccttg cccgtccaga acgcgacccg
     1741 agagctcgcg ggctccagag gcgagtcggg caacgccctc caggtccgcg tctcggggtc
     1801 gtaggcggcg gcctgggggc cgcggggcgg ggcgacgtcg tcggacccgc cgccccacac
     1861 gaccatctcc tcgcccgtcc agaccgccgc gtggccgtgc cttctcccga gggggccgag
     1921 cgggaacgcc tcccactcgt tcgtcgcggg gtcgtacgcg gccgctacgc cgcgggggcc
     1981 ggggccgccc ggccacactc cgccccagac gagcatctcc gtcccagtcc acaccgcgct
     2041 gtggctcccg aggctccacg acggcgccgg cggaagcgtg cgccagcgcc tcgtcgccgg
     2101 gtcgtacgcc gcgccgcggt gcggctggtc gtcgccgtcc tcgccgcccc agaagatcag
     2161 ctcgcgaccg gtccagacga cggcagcgcc gtggatctcg gagctgggcc cggtcggcag
     2221 cttctcccac gagtccgtgg cggggtcgaa cgaggcaccg tcgtcgaagt tctcccggtc
     2281 ggcggacccg gcgacgacga ggagctcgga tcccgtccac gcgaccgccg cgcccgagcg
     2341 accggcaatg ggagcggccg ggagagcctc ccaatgccgg agcgtcccgg gcgcggctgc
     2401 gcgcaccggt ggtttctcgc ccccgtcgag atcgatgcgc gacgtcgcga cggcgactcc
     2461 cgccgcaagc gccagcgagg tggtggctgc gcccactcgc gtcagccacc accgccgccg
     2521 gccccgcctc gacagcgcgc ggacgtccgc ccacggaggc tcgaacgcct cgcccgcgtc
     2581 cttgatcgcg atcgacagct gctcgtcatg catggttgac ctccaaggtc tttcggagcg
     2641 ccgtgcgggc atgcgtcagg tgcgcgcgca ctgcgctctc gctgatcgcc atcaagtcgg
     2701 cgatggcggc gacgggcagg tcgttccagt agaagaggac gacggcctcc cgctgccgga
     2761 acggcaggcg accgagcgcg gccaccgtgt cgacctctcg cggcacgtcg cgtcccggcc
     2821 cggcttcgga ctcgagccgc ggtgcgggcc gtccgcgccg ccggagctcc ttgcggcaca
     2881 ggttcagcgc tgtcgtcgtg acccagccgc cggcccatgg atgcttcgcc aagcgcgacc
     2941 agcgggcgta ggcacgtccg aaggcctcct gcgtcgcgtc ggacgcgacg tcggcgtcgc
     3001 cggtcgcgag gcacgcggcc cggaacaccg tccggtactc ccctcggaag aactcctcga
     3061 agtccagctc gtccccctgc ccgatcggcc ccgttcggaa tagtggaaaa cgcttccggt
     3121 tgttcaagtc gccggggccg ccaaaggctt aagactgcgc ggcccccatc ccggatctct
     3181 ggaggaaacc cgcttcgtat ggccgcgacg ctcgcccgca cgttcgaatc gctcggcgtg
     3241 aagaactatc ggatgttctt caccgggcag gtcgtgtcct ggaccggcac gtgggtccag
     3301 tggatcgcac agggctggct cgtcctgcgc ctgacgcaga gcgggctcgg cctcgggctc
     3361 gtaaccgccc tgcagtggct cccgatcctg ctgctggggg cgtgggcggg cgtcgtcgcc
     3421 gaccgtctcg acaagcgcaa gctgctgatc ttcacgaacg tcagctccgg cgtcctctcg
     3481 ctggtcctcg gcgtcctcac ggtcgccggg gtcgtcaccc tgtggatggt gatcgcggtc
     3541 gcgctgttgc tcggcgtcgt gaccgcgctg gacaaccccg cgcgccagac gttcacgatg
     3601 gagatggtgg gccgggccaa gctcacgaac gcggtcagcc tcaacaccgc gacgttcacg
     3661 accgcgcgcg tgctcggccc ggcgatcgcc ggcgtgacga tctcgaccgt cggcatcggc
     3721 ccgtgcttcc tgctgaacgc gatctcgttc gtgccggtca cgctcgcgtt gatcgccatg
     3781 gaccgttcgc agctgcgtcc gagcgaagcc gtcgcgcgca gcaagggcca ggtgcgcgag
     3841 gggctgcgct acgtgcgctc ggttccggtg ctgaagacgc tgctggtgat gatggccgtg
     3901 atcgggacgc tccagtacaa cttcgcggtg ctgctcccgg tgctcgccaa ggacacgttc
     3961 gccggcgacg cggggacgct cgggctcatc ggcgcggcga tcggcgtcgg gatgttcgcc
     4021 gggtcgatga ccaacgccgc gttcgggcgc agcgaccggc gcgtgctcct cgccgccggg
     4081 ttcgcgctcg ggacgttcac cgtcctcacc gcggccgcgc cctcgctgtg gagcgcggtg
     4141 ttgctcatgc tcccgttggg ggccgcgtcc atggccttcc tcgcgacgat gaactcgacc
     4201 ctccagctga cgtcgtccga cgagatgcgc gggcgtgtga tggcgatcta cttcgtgctg
     4261 ttcctcggct ccacgccgat cggtgcgccg atcgttggct ggctctcgga gcagttcgac
     4321 ccccgcgtgg cgctcgggtt cggcggcgtc gcgacgctcc tcgcgtgcgt ctacgggctc
     4381 ctgcggctcc cgccccttga cacccgcccg gcgacggagg acccggagga ggagctcgtc
     4441 gtcgcgagtt gaccgtccgg cgtcagaagg aatcgccccg cgtccaacga accttcccga
     4501 cgtgggcccc cgcggacggg gccgaggagc tgcaatcaga agggggaagt gatgaagaag
     4561 ctggtagggg cgtgcgtagt tgcgcttctc gtcggcgcga tggctctgcc ggcgggcgcg
     4621 aaggcagcaa agccgacggt ggcattcgag gacgccgcgg gtgacgcggg ggtccagaac
     4681 tcgtccacgc cgggcgctgc ggaggccggt ttcgacctga tcaagggcga gatcagccag
     4741 gacaagaagg gcggcgacgt caagttcgtc gtcagccacg cgtcgatgcc ccccacgtcg
     4801 cagcctgcag aggccttccg cttcatctgg ggcttcgcgg tcgacaccga gcagtacgag
     4861 ttcacggtca agagcatcga cgtcggcgag cccgacgtga tcacctccgg catcacgcag
     4921 gacccccacg gcgaggagcg cgtcggccag gtgtaccagg gcgtcgcccg tctcgagacg
     4981 tgcggttccg ttgcggctcc ctcgctgacg ctcgccacgt gcgacgcgca ggagtacttc
     5041 acggcgacgt tcgaccccgc tgccggcacg gtcacgtggt cggtgcccct ggcctcgatc
     5101 ggcgccaaga agggcagcct catcacgggc ggcgggggca cgcgtacggc gaccggctgc
     5161 cagatctgct gggtgcctca ctacctcgag cgttcgctga ccccgcacac gatcgtcgac
     5221 gcggcagcgc agctcaagag ctacaaggtc aagtaggact cacacgtttc gcaacggacg
     5281 cggccccctc gggggccgcg tcttcgcgtc tggtaggaag tcggagctga tgaccgcggg
     5341 gagccccgac gagggggcaa ggaggtacgt catgcgaaag aagctcgttg cgcctgcgtt
     5401 gatagcgacg gcgctggcgg tgtggccggc gtcgccggcg ggggccacgc acaacccgtg
     5461 catcccgtcg gaccaggtct gcatccggat ccagtgcccg aacccgtcgc acggggcctt
     5521 ctacatctac atcgactccc ggggcctgcc gttcgtcgac ttcacggact gcgcagtcgc
     5581 ctaagcgctc ggaccggttt ggaggacgcg gccccctcgg gggccgcgtt cgactttcgc
     5641 gaaggcttcc gtcggcttgg cccgcgccgg gaccgaccac ggccccccta cgtgcagcac
     5701 ccctacctcg ccgccgtgac ggttgagcca cgacacgacc gcacggacct ctcgttgcac
     5761 ggccgccgtc agccactcgc cgacgggctc ccaagtcgcc gtcttgcgca acgacgccag
     5821 gacggcatct acggttcggt cacccgcgtc gcacgccgat gccagctgtc cctcgaccag
     5881 caggatcgcc cggcccccca ccagtaacag gacctcgccc gcgtcgatca atgcccgcgc
     5941 ctcgatcgag cgctcgatcc agcgcgccag cgaccgcgct cggtcgcgta gctcggccgc
     6001 ctcttcgaac cgctcctgtc gtgcgagacg ccgcatcttg t
//
LOCUS       DASQDO010000046         5769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_740391, whole genome shotgun sequence.
ACCESSION   DASQDO010000046 DASQDO010000000
VERSION     DASQDO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740391"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VEV43_01525"
     CDS             complement(<1..281)
                     /locus_tag="VEV43_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HYP22228.1"
                     /translation="MAMTKKSTPLQRRHHRVRKKVVGSPERPRLAVYRSNRHIYAQVI
                     DDVAGRTLAASSTLAVSGGEDPKAKAKAVGAQIAEKAKAAGVTRVSFDRG"
     gene            complement(284..829)
                     /gene="rplF"
                     /locus_tag="VEV43_01530"
     CDS             complement(284..829)
                     /gene="rplF"
                     /locus_tag="VEV43_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HYP22229.1"
                     /translation="MSRIGQAPITVPSGVDVTVGSGSVRVKGPKGELSRSIPEGITIE
                     QSDGELRVVRSGESTQVRALHGLVRSLVANMIQGVTEGYEKRLEIHGVGYRAAKAGND
                     LELSVGFSHTVRKAAPSGIEFDVPQPTRITVRGIDKELVGQTAADIRAIKKPEPYKAK
                     GIRYEGEHIRRKAGKAAKAGG"
     gene            complement(840..1238)
                     /gene="rpsH"
                     /locus_tag="VEV43_01535"
     CDS             complement(840..1238)
                     /gene="rpsH"
                     /locus_tag="VEV43_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HYP22230.1"
                     /translation="MSMTDPIADMLARIRNASTAMHDDVTIPASKIKASIAKILAEEG
                     YVDAVETVEVEGHPAIKIKLRYSETRERAISGIRRISKPGRRVYRGSQELPRVLGGLG
                     VAIISTSHGVMTDKQARRAKVGGEVLAYVW"
     gene            complement(1241..1426)
                     /locus_tag="VEV43_01540"
     CDS             complement(1241..1426)
                     /locus_tag="VEV43_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HYP22231.1"
                     /translation="MAKKSLRVKAERKPKFKVRAYTRCQRCGRPRAVYRSFGLCRLCF
                     REMAHTGEIPGVTKASW"
     gene            complement(1431..2009)
                     /gene="rplE"
                     /locus_tag="VEV43_01545"
     CDS             complement(1431..2009)
                     /gene="rplE"
                     /locus_tag="VEV43_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HYP22232.1"
                     /translation="MATTTYTPRLKERFHEEIAGQLKSELGLGNIMQVPRPVKISVNM
                     GVGEAPKDAKMLEGAVKDLTAITGQRPAIRRARKSIATFKIREGMPIGAAVTLRGDRM
                     WDFLDRLMAIVFPRIRDFRGLNPKSFDGSGNYSFGLNEQLVFPEIDYDDIDATRGMDV
                     TIVTTAKDDAEGRALLTALGFPLRQQGSRQEA"
     gene            complement(2018..2335)
                     /gene="rplX"
                     /locus_tag="VEV43_01550"
     CDS             complement(2018..2335)
                     /gene="rplX"
                     /locus_tag="VEV43_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HYP22233.1"
                     /translation="MKIRKDDRVQVIAGKDVGAEGRVLEVLPRKGRVIVEGVNRVTRH
                     EKVKMNRRGGQEGGIAHKEAAIDISNVAILCPTDGPTRIGYRIEEGTGAKVRICRKCG
                     TEL"
     gene            complement(2345..2713)
                     /gene="rplN"
                     /locus_tag="VEV43_01555"
     CDS             complement(2345..2713)
                     /gene="rplN"
                     /locus_tag="VEV43_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HYP22234.1"
                     /translation="MIQQESRCRVADNTGAREVLVIKVLGGSRRRYAGVGDIVVCTVK
                     DAIPGGAVKKSDVVKAVVVRTAKERRRGDGSYIKFDDNAVVIVDGQMQPRGTRIFGPV
                     ARELREKRFMKIISLAPEVI"
     gene            complement(2710..2970)
                     /gene="rpsQ"
                     /locus_tag="VEV43_01560"
     CDS             complement(2710..2970)
                     /gene="rpsQ"
                     /locus_tag="VEV43_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HYP22235.1"
                     /translation="MTKERGKRKSRTGRVVSDVMDKTVVVEVRDAAAHPTYGKIVRRN
                     KRLKAHDEANDAHVGDTVRITETRPLSRTKRWRVVEIIERAK"
     gene            complement(2988..3194)
                     /gene="rpmC"
                     /locus_tag="VEV43_01565"
     CDS             complement(2988..3194)
                     /gene="rpmC"
                     /locus_tag="VEV43_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HYP22236.1"
                     /translation="MKASEIRDLPLDDIRQRLVDTKEELFNLRFQNATGQLDNYKRLT
                     QLRKDVARLKTILREHELAQEGNA"
     gene            complement(3194..3619)
                     /gene="rplP"
                     /locus_tag="VEV43_01570"
     CDS             complement(3194..3619)
                     /gene="rplP"
                     /locus_tag="VEV43_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HYP22237.1"
                     /translation="MLMPKKVKHRKQQRGRMRGQSKGGTTVNFGDYGLMAIEPGWITN
                     RQIEAARIAMTRHIKRGGKVWINIFPDKPYTKKPAETRQGSGKGNPEGWVAVVKPGRV
                     MFEIGGIPEPLAREAIRLAAHKLPIKSRFVLREQPMEVQ"
     gene            complement(3622..4572)
                     /gene="rpsC"
                     /locus_tag="VEV43_01575"
     CDS             complement(3622..4572)
                     /gene="rpsC"
                     /locus_tag="VEV43_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HYP22238.1"
                     /translation="MGQKVHPYGFRLGIHTDWKSRWFTEKQYKDYLVADLRIREYLMG
                     QLPHAGISRIDIERTRERVRIDAHTARPGIVIGRRGAEAERLRSQLELMESKIYGKPT
                     DVRLNIIEVKQPELDAQLIAQGVAEQLVSRVSFRRAMKKAVGTAMRHGAKGVRVQCGG
                     RLGGAEMSRTEWYIEGQMPLHTLRADVDYGFAEARTTFGRIGVKVWIYKGPFQDVYAA
                     ARDSARLRREAALAAGETVGRGAEERKSRGQTATGKPRRTSSARGASSSPPKATAPDL
                     TASTPPAGASETPSAAKPKDVKATPAADTSDGSTNSGGES"
     gene            complement(4572..5150)
                     /gene="rplV"
                     /locus_tag="VEV43_01580"
     CDS             complement(4572..5150)
                     /gene="rplV"
                     /locus_tag="VEV43_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HYP22239.1"
                     /translation="MARKIAPDAQEAIATARYIRISPTKARQVVDLIRNRHVDDARRV
                     LRFNERAASTTVAKVLDSAIANAEHNRGLPADELVVARAWVDEGPTLRRFRPRAMGRA
                     TRVRKRTCHISVVVGRPEDVVEQLPEAPTSKQTAKRVRKATTGGPKTDASATATSQAT
                     EASPETTSKKKTTAKKTTKRKTTQKKTEEGAE"
     gene            complement(5150..5434)
                     /gene="rpsS"
                     /locus_tag="VEV43_01585"
     CDS             complement(5150..5434)
                     /gene="rpsS"
                     /locus_tag="VEV43_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HYP22240.1"
                     /translation="MPRSLKKGPFVDDHLLLKVDAMNKRNEKRVIKTWSRRSTVIPEM
                     VGHTIAVHDGRKHVPVYVTESMVGHKLGEFAPTRAFRTHGGSAERSTRVR"
     gene            complement(5437..>5769)
                     /locus_tag="VEV43_01590"
     CDS             complement(5437..>5769)
                     /locus_tag="VEV43_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HYP22241.1"
                     /translation="KEGARATLRLPSGEMRMVQSECRATVGEVGNAEAELISIGKAGR
                     NRWKNKRPSVRGVAMNPVDHPLGGGEGKSSGGRHPVSPWGKKEGRTRKSKPSDDMIIR
                     RRGKKRGR"
BASE COUNT          710 a         2059 c         1904 g         1096 t
ORIGIN      
        1 ccgcggtcga acgacacgcg cgtgacgccg gcggccttgg ccttctccgc gatctgagcg
       61 ccgaccgcct tcgccttcgc cttcgggtcc tcgcctccgg acaccgcgag cgtcgaggac
      121 gccgccagcg tgcggcccgc gacgtcgtcg atgacctgcg cgtagatgtg gcggttcgag
      181 cggtagaccg cgaggcgcgg gcgctcgggc gagccgacca ccttcttgcg cacccggtgg
      241 tgcctccgct ggagcggcgt gctcttcttc gtcatagcca tggctaacct cccgccttcg
      301 cggccttgcc ggccttgcga cggatgtgct cgccctcgta ccggatgccc ttcgccttgt
      361 acggctcggg cttcttgatc gcgcggatgt ccgccgcggt ctggcccacg agctccttgt
      421 cgatcccgcg caccgtgatc cgcgtcggct gagggacgtc gaactcgatc cccgacggcg
      481 ccgccttgcg gacggtgtgc gagaacccga cggagagctc gaggtcgttg cccgccttcg
      541 ccgcgcggta gccgacgccg tggatctcga gcctcttctc gtaaccctcg gtgacgccct
      601 ggatcatgtt cgccacgagg ctgcggacca ggccgtgcag cgcacggacc tgcgtcgact
      661 cgccgctccg cacgacgcgg agctcgccgt ccgactgctc gatcgtgatg ccctcgggga
      721 tcgagcggga gagctcgccc ttcgggccct tgacgcgcac cgacccggag ccgacggtga
      781 cgtcgacccc cgaggggacg gtgatgggcg cctgtccaat ccgtgacatg tggctgctcc
      841 taccagacgt acgcgaggac ttcgccgccg accttcgcgc gacgagcctg cttgtcggtc
      901 atgactccgt gcgacgtgga gatgatcgcc acgccgaggc cgccgagcac acgcggcagc
      961 tcctgcgagc cgcggtacac gcgccggccg ggcttgctga tccggcggat cccggagatc
     1021 gcgcgttcgc gcgtctccga gtaccggagc ttgatcttga tcgcggggtg cccctcgacc
     1081 tcgacggtct cgaccgcgtc gacgtacccc tcctcggcca ggatcttggc gatgctcgcc
     1141 ttgatcttcg aggccgggat cgtgacgtcg tcgtgcatgg cggtcgacgc gttgcggatc
     1201 cgtgccagca tgtccgcgat cgggtcggtc atcgacatgg ctaccagctc gccttcgtga
     1261 cgccggggat ctcgcccgtg tgcgccatct ctcggaagca gaggcggcag agcccgaagc
     1321 tccggtaaac cgcccgcgga cggccgcacc gctgacagcg cgtgtacgcc ctgaccttga
     1381 acttcggctt cctctcggct ttcactcgga gggatttctt tgccatgccc ttacgcctcc
     1441 tgcctgctgc cttgctgcct caacgggaaa ccgagggccg tcagcagcgc ccgcccctcg
     1501 gcgtcgtcct tcgcggtggt gacgatcgtc acgtccatcc cccgcgtcgc atcgatgtcg
     1561 tcgtagtcga tctcggggaa cacgagctgc tcgttgagac cgaacgagta gttgccgcta
     1621 ccgtcgaacg acttcgggtt cagcccccgg aagtcgcgga tgcgggggaa cacgatcgcc
     1681 atgaggcggt cgaggaagtc ccacatgcgg tcgccgcgca gcgtcacggc cgcgccgatg
     1741 ggcatcccct cgcggatctt gaacgtcgcg atcgacttgc gggcccggcg gatcgcaggc
     1801 ctctgaccgg tgatcgcggt gaggtccttg accgcgccct cgagcatctt ggcgtccttg
     1861 ggggcctcgc ccacgcccat gttcaccgag atcttcacgg gacgggggac ctgcatgatg
     1921 ttcccgaggc cgagctcgga cttgagctgc cccgcgatct cctcgtggaa gcgctccttg
     1981 agcctcggcg tgtaagtcgt cgtagccatg gcgtcctcta cagctccgtt ccgcacttgc
     2041 ggcagatgcg caccttggcg cccgtgcctt cctcgatcct gtagccgatg cgcgtcggcc
     2101 cgtcggtggg acacaggatc gccacgttcg agatgtcgat cgctgcttcc ttgtgggcga
     2161 tcccgccctc ctggcccccg cggcggttca tcttcacctt ctcgtgccgg gtgacgcggt
     2221 tgacgccctc gacgatgacg cggcccttgc gcggcagcac ctcgagcacg cggccctcgg
     2281 caccgacgtc cttgccggcg atcacctgca cccggtcgtc cttgcggatc ttcagcggct
     2341 tcacctagat cacctccggt gcgagcgaga tgatcttcat gaaccgcttc tcgcgcagct
     2401 cgcgggccac gggaccgaag atcctcgtcc cgcgcggctg catctggccg tccacgatca
     2461 ccaccgcgtt gtcgtcgaac ttgatgtacg agccgtcgcc gcggcggcgc tccttcgctg
     2521 tccgcacgac gaccgccttc acgacgtcgg acttcttgac ggcgccgccc gggatcgcgt
     2581 ccttgaccgt gcacacgacg atgtcgccca cgccggcgta acgccggcgc gacccgccca
     2641 gcaccttgat cacgaggacc tcgcgggccc ccgtgttgtc ggcgactctg cacctgctct
     2701 cctgctggat cacttcgccc tctcgatgat ctcgacgacg cgccagcgct tggtgcgcga
     2761 gagcggccgg gtctcggtga tgcggacggt gtccccgaca tgcgcgtcgt tggcctcgtc
     2821 gtgggccttg aggcgcttgt tgcggcgcac gatcttgccg taggtcgggt gcgcggccgc
     2881 gtcccggacc tcgaccacga cggtcttgtc catgacgtcg gagacgacgc gccctgtgcg
     2941 gctcttgcgc ttgccgcgct ccttggtcat ctgtgtctct tcagtgctca tgcgttgcct
     3001 tcctgtgcca gctcgtgctc tctcaggatc gtcttcagac gggcgacgtc cttgcggagc
     3061 tgcgtcaacc gcttgtagtt gtcgagctgc cccgtcgcgt tctggaagcg caggttgaac
     3121 agctcctctt tcgtgtcgac cagtcgctgc cggatgtcgt ccagcggcag gtccctgatc
     3181 tccgaggcct tcatcactgc acctccatcg gctgctcgcg cagcacgaac cgcgacttga
     3241 tcgggagctt gtgcgccgcg agacggatgg cctcgcgggc gagcggctcg gggatcccgc
     3301 cgatctcgaa catcacgcgg cccggcttca cgaccgcgac ccagccctcg gggttgccct
     3361 tgccggagcc ctgacgggtc tcggcgggct tcttcgtgta cggcttgtcc gggaagatgt
     3421 tgatccagac cttcccgcca cgcttgatgt ggcgggtcat cgcgattcgg gccgcctcga
     3481 tctgccggtt cgtgatccag ccgggctcga tcgccatgag gccgtagtcg ccgaagttga
     3541 cggtggtgcc gcccttggac tggccgcgca ttcgtccgcg ctgctgcttg cggtgcttga
     3601 ccttcttcgg catcaacatg gctacgactc accacccgag ttcgtcgagc cgtcgctggt
     3661 gtcggcggcc ggcgtggcct tgacgtcctt gggcttcgcc gccgaggggg tctcggaggc
     3721 gcctgccggc ggagtgctcg cggtcaggtc gggagccgtc gccttcgggg gcgacgacga
     3781 agccccgcgg gcggagctcg tccggcgcgg cttgcccgtc gcggtctggc cgcggctctt
     3841 gcgctcctcg gccccccggc cgacggtctc gccggcggcc agcgcggcct cgcggcgcag
     3901 ccgggcggag tcgcgggccg ccgcgtagac gtcctggaac ggacccttgt agatccagac
     3961 cttcacgccg atgcggccga acgtcgtgcg ggcctcggcg aagccgtagt cgacgtcggc
     4021 gcgcagcgtg tgcagcggca tctggccctc gatgtaccac tcggtgcggc tcatctcggc
     4081 gccgcccagc cggccgccgc actgcacgcg cacgcccttg gcgccgtggc gcatcgcggt
     4141 accgactgcc ttcttcatcg cgcgccggaa agagacgcgg ctcacgagct gctcggcgac
     4201 accctgcgcg atcagctggg cgtcgagctc cggctgcttg acctcgatga tgtttagccg
     4261 gacgtccgtc ggcttgccgt agatcttgga ctccatgagc tcgagctgcg agcgcagccg
     4321 ctccgcctcg gctccacggc gtccgatcac gatccccggg cgagccgtgt gggcgtcgat
     4381 gcgaacccgc tcgcgcgtcc gctcgatgtc gatgcggctg atcccggcgt gcgggagctg
     4441 ccccatcagg tactcgcgga tgcgcaggtc ggcgacgagg tagtccttgt actgcttctc
     4501 ggtgaaccag cgggacttcc agtcggtgtg gatgccgaga cggaagccgt acgggtggac
     4561 cttctgaccc attactcagc cccctcctcg gtcttcttct gcgtggtctt gcgcttggtc
     4621 gtcttcttcg cggtcgtctt cttcttgctc gtggtctcgg gcgacgcctc ggtggcctgc
     4681 gaggtcgcgg tcgccgacgc gtcggtcttc gggccgcccg tggtcgcctt gcgcacccgc
     4741 ttcgcggtct gcttcgaggt cggggcctcg ggcagctgct cgacgacgtc ctccggacgc
     4801 ccgacgacga cggagatgtg gcacgtccgc ttgcggaccc tcgtggcacg gcccatcgcc
     4861 cgcggccgga agcggcgcag cgtcggcccc tcgtcgaccc aggcgcgggc gacgacgagc
     4921 tcgtccgccg ggaggccccg gttgtgctcg gcgttcgcga tcgcggagtc gaggaccttc
     4981 gcgaccgtcg tcgacgcggc gcgctcgttg aacctcagca cgcgccgggc gtcgtcgacg
     5041 tgacggttgc ggatgaggtc gacgacctgg cgcgccttcg tcggcgagat gcggatgtac
     5101 cgcgccgtcg cgatggcctc ctgtgcgtcg ggagcgatct tgcgagccat tacctcaccc
     5161 tcgtcgagcg ctcggcggag ccgccgtggg tgcggaacgc acgcgtcggc gcgaactcgc
     5221 cgagcttgtg gccgaccatc gactccgtga cgtagacggg gacgtgcttg cgcccgtcgt
     5281 gcaccgcgat cgtgtgcccg accatctcgg ggatcaccgt cgagcgtcgc gaccaggtct
     5341 tgatcacgcg cttctcgttg cgcttgttca tcgcgtccac cttgagcagc aggtggtcgt
     5401 ccacgaacgg gcccttcttc aagctacgcg gcattgctaa cgacctctct tcttgccacg
     5461 ccgccggatg atcatgtcgt cggacggctt cgacttgcgc gtgcggcctt ctttcttgcc
     5521 ccaggggctg accgggtgac ggcctccgga cgacttgccc tcgccgccgc cgagcgggtg
     5581 gtcgaccggg ttcatagcga caccgcggac cgaagggcgc ttgttcttcc accggttgcg
     5641 cccggccttg ccgatcgaga tcagctcggc ctcggcgttg ccgacctcgc cgaccgtggc
     5701 gcggcactcg ctctggacca tccgcatctc acccgagggg agtcgcaggg tcgcccgagc
     5761 accttcctt
//
LOCUS       DASQDO010000047         5642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_556883, whole genome shotgun sequence.
ACCESSION   DASQDO010000047 DASQDO010000000
VERSION     DASQDO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556883"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VEV43_01595"
     CDS             complement(<1..186)
                     /locus_tag="VEV43_01595"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694324.1"
                     /note="catalyzes the formation of 2-oxobutanoate from
                     L-threonine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HYP22242.1"
                     /translation="MKTHQVELVGLDDIRAARERLRGVAVQTPVDRSRALSATCRSEL
                     FVKCENLQRTGSCKIRGA"
     gene            227..1576
                     /locus_tag="VEV43_01600"
     CDS             227..1576
                     /locus_tag="VEV43_01600"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HYP22243.1"
                     /translation="MTAWATTLTPSSWTAHPAEQQPEWPDEAALKEVLEELRVLPPLV
                     FAGEARSLQAALGRVYRGEAFLLQAGDCAEAFGDFTADSIRDKLKVILQMAVVLTYAS
                     GLPVVKVGRIAGQFAKPRSSSHEVVGDTSLPSFRGHMINDSPFDAAARLPDPGRLIRA
                     YHQSAATLNLIRAFTKGGFADLRQIHVWNQEFVAASRQGQRYEVIAGQIERALRFMAA
                     CGVDLDNDAAMHQVDLFTSHEALLLGYEEALTRRDSLTGDWYDCSAHLLWIGERTRQL
                     DGAHVEFLSGVRNPVASKVGRSATPDEVVGLCERLNPEREPGRMTLIARMGAGRVEES
                     LPPLLRAARDEGHPVVWACDPMHGNTFTTEGGLKTRRFDDILAEIKAFFAACVAEGVT
                     PGGVHLELTAEDVTECLGGAEEILDLEDRYHALCDPRLNARQALDLAFQVGEMLQAR"
     gene            complement(1573..2106)
                     /locus_tag="VEV43_01605"
     CDS             complement(1573..2106)
                     /locus_tag="VEV43_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22244.1"
                     /translation="MIARRRPVLELAGIAWTAAAALVVLSLIVRRETSLSSAIASTIQ
                     VAGVVMLVAAVLGILWGLVSLARVLSWEGDLFGAGVLWAWSAGIVLLPLLFLGAYLLL
                     PALVALIVRGRLGGIAVRLPAVAVVAGLATAGVGLGWPEGARWTVLHYAVSFLGASVE
                     GAALIWFGRKTDSSSRA"
     gene            complement(2110..2295)
                     /locus_tag="VEV43_01610"
                     /pseudo
     CDS             complement(2110..2295)
                     /locus_tag="VEV43_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            2436..3047
                     /locus_tag="VEV43_01615"
     CDS             2436..3047
                     /locus_tag="VEV43_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYP22245.1"
                     /translation="MSMVMIVVLAAVGLLLLFLILTYNRLVKLRNRIDAAWSQIDVQL
                     KRRHDLIPNLVETVKGYAAHERETLEAVVAARRQAVDATGVEQQAQAENMLTGALRQL
                     FALAEAYPDLKANQNFLALQEELTGTEGRIAFSRQFYNEQTLAYDNARESFPTNVVAG
                     AFNFEAREYFEAEESSREPVRVDFGQGGGTGAPPPAGAPGTTA"
     gene            3061..3993
                     /locus_tag="VEV43_01620"
     CDS             3061..3993
                     /locus_tag="VEV43_01620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYP22246.1"
                     /translation="MYDRIAGNVRKSWMLIIVFFVLTGAVGYFFGYFTGTGLWGLWIA
                     VTFSVFMSWGSWFASDKVALSMSRARPADEKLFAQLHNIVESLSLAAGLPKPRVYVVE
                     DIAPNAFATGRNPEHAAIAVTTGLVLKMKRDELEGVIAHELAHVKNKDTLVMTLAVTL
                     VGVIVLLADWMLRGLMWGAGEAEGKAFAPLAILGVVLLITAPITAQMLQFAISRRREF
                     LADADAVFLTRYPDGLINALEKLRTDSTVVRSASRATAHLWIEQPNARHAHEGGTKTR
                     TSGAWLNRLFDTHPPLEERIAALRSLAPQPTATV"
     gene            4099..>5642
                     /locus_tag="VEV43_01625"
     CDS             4099..>5642
                     /locus_tag="VEV43_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22247.1"
                     /translation="MKRFAVFLTACMVASLGLATVPGSVTSAQTPTQGGVVLGPVEYV
                     TYLPFDNATSTGVSIHKNYMYLTSWKAISIYDISDPAAPVQTDSLPVGFMFENEDVEI
                     TPDANYLFFSETIPDEQLHVYDIEDKSNIQLVTTVENAGDHTFSCLLKCKWGYGSDGT
                     IIDLADPTQPKIAAAQGSADDWHKKLGIAGGAHDVTEVKPGYAIISTLSGPLIYADVR
                     DPMNPKLLALGEKPSEAYLFHSGEWPNGGTDRWLLMQGEQNAQPRCDPEANGPFLVYD
                     TQGWASKKTFKLSDQYAVQNGTFTDGNPPANGLGCSAHWFEEHPTFDNGGVVAMGYYE
                     HGTRFFNVTSKGKIEEAGVFLPYAGSTSAAYWASDKANTDIVYAVDYTRGIDVLRYTG
                     KTIDGGGGGGNGGNGGGAKRPYVKMAAKPYRPVRGSNFNFVVRLAACERSNLNPAGTD
                     VVLKRKKGGKYVNVARKSFDRSCRVLFPRKADFKSAKFRAFWPKQRDGYTAGRSQEIT
                     VNTRNG"
BASE COUNT          991 a         1944 c         1877 g          830 t
ORIGIN      
        1 ggcgccgcgg atcttgcagg agcccgtgcg ctggaggttc tcgcacttga cgaagagctc
       61 gctgcggcac gtcgcggaca gcgcgcggga gcggtccacg ggcgtctgga cggcgacacc
      121 ccgcagacgc tcacgcgccg cacggatgtc gtcgagcccc accagctcca cctggtgcgt
      181 cttcaccacc tgcatcgtgc cacgcccgtc cgcatctacg cgacgattga ctgcgtgggc
      241 cacgacgttg acgccttcat cgtggaccgc ccacccggcc gagcagcagc cggagtggcc
      301 cgacgaggcc gcgctcaagg aggtcctcga ggagctgcgg gtcctgcccc ccttggtctt
      361 cgccggcgag gcgcgctcgt tgcaggcggc tctcgggcgg gtctaccggg gcgaggcctt
      421 cttgctccaa gccggcgact gcgccgaggc gttcggggac ttcaccgccg actcgatcag
      481 ggacaagctc aaggtcatcc tgcagatggc ggtcgtgctg acgtacgcgt cggggctgcc
      541 ggtcgtgaag gtgggccgga tcgcgggcca gttcgcgaag ccgcgctcgt cgtctcacga
      601 ggtcgtcggc gacacgtctc tgccctcgtt ccgcgggcac atgatcaacg actcgccgtt
      661 cgacgcggcc gcgcggctgc ccgatccggg acggctgata cgtgcgtacc accagtcggc
      721 cgcgacgctc aatctcatcc gcgcgttcac caagggcggg ttcgccgacc tgaggcagat
      781 ccacgtctgg aaccaggagt tcgtcgcggc atcgcggcag ggtcagcgct acgaggtgat
      841 cgccgggcag atcgaacgcg cgctgcgctt catggccgcg tgcggggtcg acctcgacaa
      901 cgacgccgcg atgcaccagg tcgacctctt cacctcgcac gaagcgctct tgctcgggta
      961 cgaggaggcg ttgacgcggc gcgacagcct caccggcgac tggtacgact gctccgcaca
     1021 cctgctgtgg atcggcgagc gcactcgtca gctcgacggc gcgcacgtcg agttcctgtc
     1081 cggcgtccgc aaccccgtcg cgtcgaaggt cggcaggagc gcgacccccg acgaggtcgt
     1141 cgggttgtgc gaacggctga accccgagag ggagccgggc cggatgacgt tgatcgcgcg
     1201 aatgggagcc gggcgcgtcg aggagtcgct gccgccgttg ctgcgcgccg ccagggacga
     1261 gggccatccc gtcgtgtggg cgtgcgaccc gatgcacggc aacacgttca cgaccgaggg
     1321 cgggctcaag acgagacggt tcgacgacat cctcgcggag ataaaggcgt tcttcgccgc
     1381 atgcgtggcc gagggcgtca cgcccggcgg ggtccacttg gagctgacgg ccgaggacgt
     1441 gacggaatgt ctcggggggg ccgaggagat actggacctc gaggaccggt accacgcgct
     1501 atgcgacccg cgcctgaacg cgcgccaggc gctggacctg gcgttccagg tcggagagat
     1561 gctccaggcg cgctaggcgc ggctcgagga gtcggtcttt cttccgaacc agatcaacgc
     1621 agctccctcg acggacgcgc cgaggaaaga gacggcgtag tggaggacgg tccatcgggc
     1681 cccctcgggc cagccgagac caactcccgc cgtcgccaga ccggccacga ccgcgacggc
     1741 cgggagccgg actgcgatgc cgccgaggcg gccgcggacg atcagggcca ccaacgccgg
     1801 gagcaacagg tacgcaccca agaagaggag cggcaaaagc acgatccctg cgctccaagc
     1861 ccagaggacg cccgcgccga agaggtcgcc ctcccaggag aggactctgg ccagtgagac
     1921 gagaccccag aggatcccca gcaccgcggc gacgagcatg acgacgccgg cgacctgaat
     1981 cgtcgaggcg atggcggagg acagggacgt ctccctgcgc acgatcaggg agaggaccac
     2041 gagcgccgct gctgccgtcc acgcgatgcc tgcgagctcg aggaccggcc gtctccgtgc
     2101 gatcaagcgc tagcggcttc ggatcgtctt caggatctcg tcgtactccg cgtggcggcc
     2161 cgtggccttc ttcgagaaca gcagggcccc gtccacctcc acctcgaaga ccccgccgcg
     2221 cgaggggact atcaccagct tggtgatctc cttctcgaac tcctccagca gcctctccgt
     2281 cagaccgacg gctctaggtg cgtagcctca ttgggcgcag tactcgatcg agaccctcat
     2341 ggcctcatcc taggcgccga gacctgatcg gcttgcttaa ccggcccccg tgcctcaatc
     2401 ttgtagcgcg accggaccaa ggagggaact cgggcatgag catggtgatg atcgtcgtcc
     2461 tggcagccgt cgggctgctg ctgctcttct tgatcctgac gtacaaccgc ctcgtcaagc
     2521 tcaggaaccg gatcgacgcg gcctggtcgc agatcgacgt ccagctcaag cgacgccacg
     2581 acctgatccc gaacctcgtt gagaccgtca agggctacgc ggcgcacgag cgcgagacgc
     2641 tcgaagccgt cgtcgccgcc cgccgtcagg ccgtcgacgc aacgggcgtc gagcagcagg
     2701 cccaggccga gaacatgctc accggcgcgc tgcggcagct gttcgcgctc gccgaggcgt
     2761 accccgacct gaaggcgaac cagaacttcc tcgcgttgca ggaggagctg acgggcacgg
     2821 agggccgcat cgcgttctcg cggcagttct acaacgagca gaccctcgca tacgacaacg
     2881 cgcgcgagag cttcccgacg aacgtcgtcg cgggagcctt caacttcgag gcacgcgagt
     2941 acttcgaggc cgaggagtcg tcgcgcgagc cggtgcgggt cgacttcggg caaggcggcg
     3001 gcaccggcgc accgccgccg gcaggcgctc ccggcaccac cgcttaagcg acggcgcatt
     3061 ttgtacgatc gcatcgcggg caacgtccgc aagtcgtgga tgctcataat cgtcttcttc
     3121 gtcctcacgg gcgcggtcgg ctacttcttc ggctacttca ccgggaccgg cctgtggggc
     3181 ctatggatcg cggtcacgtt ctcggtcttc atgtcctggg gatcgtggtt cgcctcggac
     3241 aaggtcgcgt tgagcatgag ccgggcccgc cccgcggacg agaagctctt cgcgcagctc
     3301 cacaacatcg tcgagagcct cagcctcgcg gccggcctcc ccaagccgcg cgtctacgtc
     3361 gtcgaggaca tcgcgcctaa cgcgttcgcg accggcagga accccgagca cgcagccatc
     3421 gcggtgacca cggggctcgt cctcaagatg aagcgcgacg agctcgaggg tgtcatcgcg
     3481 cacgagctcg cccacgtgaa gaacaaggac accctggtga tgacgctggc ggtgacgctc
     3541 gtcggcgtca tcgtgctgct cgcggactgg atgctccggg gcctgatgtg gggggcgggg
     3601 gaggccgaag gtaaggcgtt cgcgcccctc gcgatcctcg gcgtcgtgct gctgatcacc
     3661 gcgccgatca ccgcgcagat gctccagttc gcgatctcgc gccgccggga gttcctcgcg
     3721 gacgcggacg ccgtgttcct cacgcgttat cccgacggcc tcatcaacgc gctcgagaaa
     3781 ctacgcaccg actccacggt agtgcgctcc gcgagcagag caaccgcgca cttgtggatc
     3841 gaacagccga acgcgcgcca cgcgcacgag ggcggaacca agacgcgcac gtcgggggcg
     3901 tggctgaacc ggctgttcga cacgcacccg ccgctcgagg agcgcatcgc ggcgctacgg
     3961 tcgctcgcgc cgcaacccac cgccaccgtt taaccaatcc agggggtcgg aatcccccga
     4021 atcgggcata agcgcaggta tggcccccat ctgggcgaac caccaacgag gcgcgcgacg
     4081 attcgatttg gaggatccat gaagcgattt gctgttttcc tgaccgcctg catggtggcg
     4141 agcctggggc tcgcgacggt gccgggctcg gtcacctcgg cccagacgcc cacgcaaggt
     4201 ggcgtcgtgc tcggccccgt cgagtacgtg acgtacctgc cgttcgacaa cgcgacgtcg
     4261 accggtgtct cgatccacaa gaactacatg tacctgacga gctggaaggc gatctcgatc
     4321 tacgacatct ccgaccctgc ggcccccgtg cagacggact cgctgccggt cgggttcatg
     4381 ttcgagaacg aagacgtcga gatcacgccc gacgcgaact acctcttctt ctcggagacg
     4441 atcccggacg agcagctcca cgtctacgac atcgaggaca agagcaacat ccagctcgtg
     4501 acgacggtcg agaacgccgg cgaccacacg ttctcgtgcc tgctcaagtg caagtggggc
     4561 tacggctccg acgggacgat catcgacctc gccgacccga cgcagccgaa gatcgcggcc
     4621 gcgcaggggt ccgcagacga ctggcacaag aagctgggca tcgccggcgg cgcgcacgac
     4681 gtcaccgagg tcaagcccgg ctacgcgatc atctcgaccc tctccgggcc gctgatctac
     4741 gcagacgtgc gcgacccgat gaacccgaag ctgctcgccc tcggcgagaa gccgagcgag
     4801 gcctacctgt tccactccgg tgaatggccc aacggcggca ccgaccggtg gctgctgatg
     4861 cagggcgagc agaacgccca gccgcgctgc gaccccgaag cgaacgggcc cttcctcgtc
     4921 tacgacacgc agggttgggc gtccaagaag acgttcaagc tctccgacca gtacgcggtc
     4981 cagaacggca cgttcacgga cggcaacccg ccggccaacg ggctcggctg ctcggcccac
     5041 tggttcgagg agcacccgac gttcgacaac ggcggcgtcg tcgccatggg ctactacgag
     5101 cacgggacgc gtttcttcaa cgtgaccagc aagggcaaga tcgaagaggc cggcgtgttc
     5161 ctgccgtacg caggctcgac gtcggcggcc tactgggcga gcgacaaggc caacaccgac
     5221 atcgtctacg cggtcgacta cacgcgtggg atcgacgtcc tccgctacac cggcaagacc
     5281 atcgacggtg gcggcggcgg gggcaacggc ggcaacggtg ggggcgccaa gcgtccgtac
     5341 gtgaagatgg cggcgaagcc gtaccggccc gtccgcggca gcaacttcaa cttcgtcgtg
     5401 cggctcgcgg cgtgcgagcg cagcaacctc aaccccgccg gcaccgacgt cgtcctcaag
     5461 cgcaagaagg gcggcaagta cgtgaacgtc gccaggaaga gcttcgaccg ctcgtgtcgc
     5521 gtgctgttcc cccgtaaggc ggacttcaag tcggcgaagt tccgggcgtt ctggccgaag
     5581 cagcgggacg gctacacggc cggccgctcg caggagatca cggtcaacac ccgtaacggc
     5641 ta
//
LOCUS       DASQDO010000048         5765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_190011, whole genome shotgun sequence.
ACCESSION   DASQDO010000048 DASQDO010000000
VERSION     DASQDO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190011"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..991)
                     /locus_tag="VEV43_01630"
     CDS             complement(128..991)
                     /locus_tag="VEV43_01630"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP22248.1"
                     /translation="MSERVAFVGLGAMGSRMARRLIDAGYDLHVWNRTRAKAEALASR
                     GAVVADSPAEAASSADAVITMVADPAALADVSEDPSGIAVGVREGTVVIEMSTVGPAA
                     IARLREALPRGTDLVDAPVLGSLSEAEAGELRVFVGGSAGSFERVRPLLEAMGSPLHA
                     GDLGAGAAAKLVANSTLFGSLGVLGEALALADSLGLDRDTAFEVLSGTPVAAQAERRR
                     GPIESGEFPLRFALSLALKDADLVTTAGPELRLAAAARSWLAGAEAAGLGDADYSRVL
                     THIIDEIRRTT"
     gene            complement(988..2322)
                     /locus_tag="VEV43_01635"
     CDS             complement(988..2322)
                     /locus_tag="VEV43_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP22249.1"
                     /translation="MRRRLAVSMLALLVLQSVPVAAQETPECFGRPATITGTDGHDVI
                     RGTDDDDVIVSGDGADYVLAKGGDDLVCAGPGRQVHYDEEWQEYTYDGDSVEGGPGND
                     RIDGGPDLNELSGGSGDDLLLGHDDGDNLSGDDGNDTIEAAEGHNTVYGGDGADAITS
                     GAGDDELSAGRGDDRVTAGAGDDEVFPMQGHDTSDAGEGHDMLNLFWESCDGWCFSSH
                     RRDLDVDLVAGFANGMGNDMIAGYEDVWGGSGSDVIRGDDGPNLLSAEDDQGEGDAPN
                     VLDGRGGDDRLVSGEGDDEISGGDGRDELSFTSNVWRVRVDLRAGTVTGGTSDTISGI
                     EDVSAASGGVLIGDDGPNRLEAFYGADVVRGGGGDDEISGGSHGDTLKGGPGHDVLSG
                     GARHDSLRGGTGDDVLDGGKGENSNHGGPGTDACARPGPGEGAVACESEAPP"
     gene            2393..2542
                     /locus_tag="VEV43_01640"
     CDS             2393..2542
                     /locus_tag="VEV43_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22250.1"
                     /translation="MRRMAAVLVASALSFGILTAGAAPAHACVDLRCQIACIKAILSG
                     GPCPD"
     gene            complement(2549..>5765)
                     /locus_tag="VEV43_01645"
     CDS             complement(2549..>5765)
                     /locus_tag="VEV43_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22251.1"
                     /translation="VAGRSRHTPSWSPDGRYLAVDDRRTVYRIDTQAGHEIALTQPEE
                     TTLICLGQEFTFPVWYHQPSWSPEGARIAVIRYHEVAPSPNMGSCDFAPFFDYDLATI
                     PAAGGDVVMLTDDPSTVSETRFDALPDWSPDGSKIVFQRARPGSAIGIAVVSAGGGAP
                     AQLAPFGARPRWSPDGAKVLFTRDSDDDGFSEIWTMDASDGSDAAALLRRDGVGYAYQ
                     DWQPTTAGELEVTLDAQAPDGTSLLGDVVGMSQTFLVRLTIRNPGDEEVTGFRFAGDA
                     PLVIDERSTGGLEITAAPPVDESLTLEPEEEVTFEFEVTATADGIAAAHTKVSATDES
                     GRLLEDVHSLKFVIQDGAAMTDALGQYVILQAMDALVLQSARSWYAGMAARGIDLQQR
                     LSAIFTPEQRRRWFGSETGFPLTPTDFAIALLRGTTAEMVAASTPKTAPDGLTIERLN
                     RAYDRTFKDEVGKGVEEYVQGWANLARGAKKALRDSYSEALLTSYYLLGNATPEERLQ
                     VEAYMYDAATGTDASVDNLVNTVAREIPRWKENGTYLDQALDMAVADAFLLSPDLQNQ
                     MAAETQWRENVLARAQSDPVAFQEAVAKRDAEIFNLGLPLILDTLIGGGVTRVDVGAR
                     NVVVRGRGASVLRTGEAVGALDGSGRVSNRPLKVRPNDSSTPGGVTTSEAVRLERSGS
                     YLDDVEGATVIQSTDAGSVYELPNLSGVPEVTLEAKAGILGELESEYARRFGTQIKLA
                     EVLKPSSALRKPGGVAKLELTGQKTGKPAMLDAGAPDEILGEANVWRNTRRPPELPGW
                     GDLSKPRQEAAMKEWRLANQRWEEWVDPPAGSKTARLKECIGRRSRVPLDAQPNAAGI
                     QRFVEAEFEEVVVREGSAEARLIRAKYYEIETVDTTRGNRVVNSKVVVDSAEALPQTP
                     DADAVALGKVTGTRPDGSPVVSPLSRAEREFLAQRYIDKNVKARRKPPGTPGAIPDAA
                     EHGTTLIMDDASAQAAGKLLPSYGVPFLPETVGRSLLQRIAPFVAKIPPGTGPAQRAR
                     IIRAQYEQMLHAVRSEGGFGQHAVVVTSDSRYLGAVQFESW"
BASE COUNT          768 a         2130 c         1943 g          924 t
ORIGIN      
        1 ctcgaggatg ctcgcgtcga cctcggccgc ggccgcgtcg attcgctctc tctccgcggg
       61 cgtcaaggac atctcgggca taggttcagc atcggcctcc gcacacaccc gcttcaagcg
      121 gatacgtcta cgtagttctg cgtatctcgt cgatgatgtg cgtcaagacc cgtgaatagt
      181 cggcgtcgcc gagccccgcg gcctccgcgc cggcgagcca gctccgggcc gctgccgcca
      241 ggcgcagctc cggtcccgcc gtcgtgacga ggtccgcgtc cttgagcgcc agcgacaacg
      301 cgaaccgcag cgggaactcg ccgctctcga tcgggccgcg gcggcgttcc gcctgcgcgg
      361 cgaccggcgt gcccgacagg acctcgaacg cggtgtcccg gtcgaggccg agcgagtccg
      421 cgagcgctag cgcctccccg agcacgccga gcgagccgaa gagcgtgctg ttcgcgacga
      481 gcttcgccgc ggcccccgca ccgagatcgc ctgcgtgcaa aggcgacccc atcgcttcga
      541 gcaggggacg gactcgctcg aaggaacctg cggagccacc gacgaacacg cgcagctcgc
      601 cggcttcggc ttccgacagg ctgccgagca ccggggcgtc gacgaggtcg gtgccgcgcg
      661 gcagggcctc acgcagacgc gcgatggccg cggggccgac cgtggacatc tcgatgacga
      721 ccgtgccctc gcgcacgccc acggcaatcc ccgacgggtc ttcgctcacg tctgccagcg
      781 ccgcgggatc ggcgaccatc gtgatcaccg cgtccgcgct cgaagcagct tccgccgggc
      841 tgtctgcgac gacggcgcct cgcgacgcga gcgcctcggc cttcgctcgg gtccggttcc
      901 atacgtgcag gtcgtagccg gcgtcgatca gccggcgcgc catgcggctc cccatcgcgc
      961 cgagcccgac gaacgcgacg cgctcgctca cggcggtgcc tccgactcgc acgcgaccgc
     1021 accttcgccc ggcccgggcc gagcgcacgc gtccgtcccg ggccccccgt ggttcgagtt
     1081 ctcgcccttg ccgccgtcga gcacgtcgtc acccgtgcca ccgcggagac tgtcgtggcg
     1141 ggcacccccg gacaggacgt cgtggccggg gccgcccttc aacgtgtcac cgtgcgatcc
     1201 gccggagatc tcgtcgtcgc ctccgccccc gcgaacgacg tcggcaccgt agaaggcctc
     1261 gagccggttc ggcccgtcgt cgccgatcag cacgccccca ctcgccgccg atacgtcttc
     1321 gatcccggag atcgtgtcgc tcgtcccgcc ggtgacggtc ccggccctga ggtcgacgcg
     1381 caccctccag acgttgctcg tgaaagagag ctcgtcgcgt ccgtctccgc cggagatctc
     1441 gtcgtcgccc tcgcccgaca cgagacggtc gtcaccgccg cgcccgtcga gcacgttcgg
     1501 tgcgtcgccc tcgccctggt cgtcttccgc gctcaacagg ttcggcccgt cgtcgccgcg
     1561 gatgacgtcg ctccccgagc cgccccacac gtcctcgtac ccggcgatca tgtcgttccc
     1621 catcccgttg gcgaacccgg cgacgaggtc gacgtcgagg tcccgcctgt gggagctgaa
     1681 gcaccagccg tcgcagctct cccagaagag gttgagcatg tcgtggccct ccccggcatc
     1741 gctcgtgtcg tggccctgca tcgggaagac ctcgtcgtcg cccgctccgg cggtcacccg
     1801 gtcgtcgccg cggccggcgg agagctcgtc gtcgccggca ccgctcgtga tcgcgtccgc
     1861 accgtcgcct ccgtagaccg tgttgtggcc ttcggcggcc tcgatggtgt cgttgccgtc
     1921 gtcgccggag aggttgtccc cgtcgtcgtg accgaggagc aggtcgtcgc cggaaccgcc
     1981 cgacagctcg ttcaggtccg ggcccccgtc gatccggtcg ttgccgggac cgccctcgac
     2041 ggagtcgccg tcgtacgtgt actcctgcca ctcctcgtcg tagtgcacct gccggcccgg
     2101 tcccgcgcac acgaggtcgt cgcctccctt cgcgagcacg tagtccgcgc cgtcgcccga
     2161 gacgatcacg tcgtcgtcgt cggtcccccg gatgacgtcg tggccgtcgg tccccgtgat
     2221 cgtcgcgggc cggccgaagc actcgggcgt ctcctgcgcg gcgaccggga cactctgcag
     2281 gaccagcagg gcgagcatcg agacggcgag gcggcggcgc atcctttgat gatggcaccg
     2341 ctcatgggaa actccgggcc gtcccgtggg cagaggagaa aggacggtgg acatgcggcg
     2401 aatggcagca gtgctcgtgg cgagtgcgct gagcttcggg atcctgaccg caggagcggc
     2461 gccggcacac gcgtgcgtcg acctcaggtg tcagatcgcg tgcatcaagg cgatcctgtc
     2521 gggcgggccc tgccccgact gaggtccgtc accagctctc gaactgcacc gctccgaggt
     2581 agcggctgtc ggacgtcacg acgaccgcgt gctgtccgaa gccgccctcg gaacggaccg
     2641 cgtgcagcat ctgctcgtac tgggcccgga tgatgcgcgc gcgttgcgcg ggccccgtcc
     2701 ccggcgggat cttcgctacg aacggcgcga tgcgctgcaa caacgaacgg cccacggtct
     2761 cgggcaggaa cggcacgccg tagctgggca acagctttcc cgccgcttgc gcgctcgcgt
     2821 cgtccatgat caaggtcgtg ccgtgctccg cggcgtcggg aatggcgccc ggcgtccccg
     2881 gcggcttgcg gcgcgccttc acgttcttgt cgatgtagcg ctgcgcgagg aactcgcgct
     2941 cggcgcgtga cagcggcgac acgacggggc tcccgtcagg ccgcgtgccc gtgaccttgc
     3001 cgagcgcgac cgcgtcggcg tccggtgtct gcggcagggc ctcggcggaa tcgactacga
     3061 ccttgctgtt gacgacgcgg ttcccgcgcg tcgtgtcgac ggtctcgatc tcgtagtact
     3121 tcgcgcggat cagccgggcc tccgcgctcc cctcgcggac gacgacctcc tcgaactccg
     3181 cctcgacgaa gcgctggatg ccggctgcgt tcggctgggc gtcgagcggg acccggctgc
     3241 ggcgtccgat gcactccttg aggcgcgcgg tcttcgatcc cgccggcgga tcgacccact
     3301 cctcccatcg ttggttcgcc agccgccatt ccttcatggc cgcctcctgg cgcggtttcg
     3361 agaggtcgcc ccatcccggg agctccggcg gccgtctcgt gttccgccac acgttcgcct
     3421 cgccgaggat ctcgtccggg gcgcccgcgt cgagcatcgc cggcttgccc gtcttctgcc
     3481 cggtcagctc gagcttcgcg accccgcccg gcttacgcag cgcgctcgag ggcttcagga
     3541 cctcggccag ctttatctgc gtgccgaacc tgcgcgcgta ctcggactcg agctcgccga
     3601 ggatgccggc cttggcctcg agcgtgacct cgggcacgcc gctgaggttc ggcagctcgt
     3661 acacgctgcc ggcgtcggtc gactggatga ccgtcgcacc ctcgacgtcg tcgaggtagc
     3721 tccccgagcg ctcgagccgg accgcttccg aagtcgtcac gccgccgggt gtcgaggagt
     3781 cattgggcct gaccttcagg ggccggttcg agacgcgacc ggagccgtcg agcgcaccca
     3841 ctgcctcgcc cgtccgcagg accgacgcgc cccggccccg gacgacgacg ttacgggcac
     3901 cgacgtcgac gcgcgtgacg ccgcctccga tcaacgtgtc gaggatcagg gggaggccga
     3961 ggttgaagat ctcggcgtcc cgcttcgcga ccgcctcctg gaacgcgacc gggtcgctct
     4021 gtgcgcgggc gaggacgttc tcgcgccact gcgtctcggc cgccatctgg ttctggaggt
     4081 cgggcgagag aaggaacgcg tcggcgaccg ccatgtccag cgcctggtcc aggtacgtcc
     4141 cgttctcctt ccagcgcggg atctcgcgcg cgaccgtgtt cacgaggttg tcgacggacg
     4201 catcggtgcc ggtggcggcg tcgtacatgt acgcctcgac ctgcagccgc tcctcgggcg
     4261 tcgcgttgcc gaggaggtag tacgaggtca gcaaggcctc tgagtaggag tcccgcaggg
     4321 ccttcttcgc gccgcgcgcg aggttcgccc agccctgcac gtactcctcg acgcccttcc
     4381 cgacctcgtc tttgaaggtc cggtcgtagg cgcggttcag ccgttcgatc gtgagcccgt
     4441 ccggagcggt cttcggcgtc gacgccgcga ccatctcggc cgtcgtcccg cgcagcaacg
     4501 cgatcgcgaa gtcggtgggg gtgagcggga acccggtctc gctgccgaac caccgtcgtc
     4561 tctgctccgg ggtgaagatc gccgagagac gctgctgcag gtcgatgccg cgcgccgcca
     4621 tccccgcgta ccaggagcga gcgctctgga ggacgagcgc gtccatcgcc tggaggatca
     4681 cgtactgacc gagcgcgtcg gtcatcgccg cgccgtcctg gatcacgaac ttgagagagt
     4741 ggacgtcctc gagcagccgg ccgctctcgt cggtcgcgct caccttcgtg tgcgcggccg
     4801 cgatcccgtc ggcggtcgcc gtcacctcga actcgaacgt cacctcctcc tccggctcga
     4861 gggtcaggct ctcgtctacc ggcggtgctg cggtgatctc gagcccgccg gtgctgcgct
     4921 cgtcgatcac gagcggtgcg tcgcccgcga acctgaaccc ggtcacctcc tcgtcgccgg
     4981 gatttcggat cgtcagccgg acgaggaacg tctgcgacat cccgactacg tctccgagca
     5041 acgacgtccc gtcgggcgct tgcgcgtcca gcgtcacctc gagctcgccg gccgtcgtcg
     5101 gctgccagtc ctggtaggcg tagccgacac cgtcacgccg caggagcgcg gccgcgtccg
     5161 atccgtcgga cgcgtccatc gtccatatct ccgagaagcc gtcgtcgtcc gagtcccgag
     5221 tgaacaggac cttggccccg tcgggggacc agcgtggccg ggcaccgaac ggcgcgagct
     5281 gagccggcgc cccgccacct gcggagacca cggctatgcc gatggcgctg ccgggacgag
     5341 cccgctggaa gacgatcttc gaaccgtccg gcgaccaatc gggcaacgcg tcgaaacgcg
     5401 tctcgctcac ggtcgacggg tcgtccgtga gcatcacgac gtcgccgccg gcggcaggga
     5461 tggtcgcgag gtcgtagtcg aagaagggag cgaagtcaca cgaccccatg ttcggagacg
     5521 gtgcgacctc gtggtacctg atcaccgcga tgcgcgctcc ttcgggggac cacgacggct
     5581 ggtgatacca gacggggaac gtgaactcct ggccgagaca gatgagcgtg gtctcctccg
     5641 gctgcgtcaa cgcgatctcg tgacccgcct gggtgtcgat ccggtagacg gtccggcggt
     5701 cgtcgacggc gagatagcgc ccgtccggcg accacgacgg ggtgtggcga ctccgccctg
     5761 cgacg
//
LOCUS       DASQDO010000049         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_556968, whole genome shotgun sequence.
ACCESSION   DASQDO010000049 DASQDO010000000
VERSION     DASQDO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556968"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VEV43_01650"
     CDS             <1..230
                     /locus_tag="VEV43_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22252.1"
                     /translation="WVGTSEQDRMFVRLERGTESPCTVRAGVRGSFSGVLAQSDAGLA
                     DQLGVTEAEGAQMLVTQGAYVDVKSGDIRSG"
     gene            complement(250..870)
                     /locus_tag="VEV43_01655"
     CDS             complement(250..870)
                     /locus_tag="VEV43_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22253.1"
                     /translation="MPSRLHPVNAVSIRQAPAPCVECVFWQGRDGRSVDKARWADKVE
                     DDWGAWGTLYFAEDDHLLGFIQAAPADHFPRGQDLPAGPPSGDAVLIACAYLVDLSAP
                     WVMQSLFLSVIGEARDRGVKAIETFAYRYPEGESGYERFFVHRTIFPSDFLADFGFRP
                     VRWDGQVALARLDLGGLVPVAEGRREVVLRKVKEAFVPAPVPQRPY"
     gene            complement(875..>1545)
                     /locus_tag="VEV43_01660"
     CDS             complement(875..>1545)
                     /locus_tag="VEV43_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYP22254.1"
                     /translation="HLGTLSAAGRALDKAVAWAQEDRPDLVAITGDLVRRPGGRGKLD
                     DALGRLPSRYGIVAILGNHDVDDARDPFSRPTDLSDLHDAGTTLLRDSEWSTDVSGVT
                     VQVVGMDPASYKLGRSRPVERADASAGLRILLCHYPEVVRRLPPGVFDLVLAGHYHGG
                     QICLPTPWGKVRLKELRGTYWEGLFATEAAAMHVSRGLGTSLVPFRFLARPEATVLTL
                     RASR"
BASE COUNT          239 a          522 c          580 g          204 t
ORIGIN      
        1 tctgggtggg cacgagcgag caggaccgga tgttcgtgcg gctcgagcgc ggcacggagt
       61 cgccgtgcac cgtgcgcgcc ggcgtgcgag ggtcgttctc gggtgtcctg gcgcaatcgg
      121 acgcgggtct cgctgaccag ctcggcgtca ccgaggccga aggcgcgcag atgctagtca
      181 cccagggcgc ctacgtggac gtgaagtccg gagacattcg ctccggctag cggtaccacg
      241 cgggcgcggc tagtacggac gctgcgggac cggcgcgggg acgaacgcct ccttgacctt
      301 gcggaggacg acctcgcgcc ggccctcggc gaccggcacg agcccgccca gatcgaggcg
      361 cgcgagcgcc acctgtccgt cccagcgaac cgggcggaag ccgaaatcgg ccaggaagtc
      421 actcgggaag atcgtccgat ggacgaagaa gcgctcgtag ccgctctcgc cctcgggata
      481 gcggtacgcg aaggtctcga tcgccttgac cccgcggtcg cgggcttccc cgatcaccga
      541 caggaagaga ctctgcatca cccacggcgc cgagaggtcc acgaggtatg cgcaggcgat
      601 caggacagcg tcgcccgagg gcgggcccgc aggtaggtct tgcccgcgcg gaaagtggtc
      661 ggccggcgcg gcctggatga agccgagcag gtggtcgtcc tcggcgaagt agagcgtccc
      721 ccacgcgccc cagtcgtcct cgaccttgtc cgcccagcgc gccttgtcga ccgagcggcc
      781 gtcgcggccc tgccagaaga cgcactcgac gcacggggcg ggcgcctggc ggatcgagac
      841 ggcgttcacc gggtgaaggc gggagggcat gggcctatct tgatgcgcgg agcgtcaaga
      901 cggtggcctc ggggcgtgcg aggaagcgaa acgggacgag gctcgtgccc aggccgcggg
      961 agacgtgcat ggccgccgcc tccgtcgcga agagcccttc ccagtaggtg ccgcgaagct
     1021 ccttcagcct caccttgccc cagggagtgg gaaggcagat ctgcccgccg tggtagtgcc
     1081 ccgcgagcac gaggtcgaag acgccgggcg gcaggcgccg gacgacctcg gggtagtgac
     1141 agaggaggat ccgaagcccg gccgaggcat ccgcccgctc gaccggccgg gagcggccga
     1201 gcttgtagct cgcgggatcc atgcccacga cctggacggt gacgccgctc acgtccgtcg
     1261 accactcgct atcgcggagg agcgtcgttc cggcgtcgtg caggtcggag aggtcggtcg
     1321 gccgcgagaa cgggtcgcgc gcgtcgtcga cgtcgtggtt tccgaggatc gcgacgatcc
     1381 cgtagcggga ggggagcctt ccgagcgcgt cgtcgagctt tccgcgcccg ccgggccgcc
     1441 gtacgaggtc gcccgtgatc gccacgaggt cgggacgatc ctcctgcgcc caggccacgg
     1501 ccttgtcgag cgcgcggccg gccgccgaga gcgtcccgag gtgca
//
LOCUS       DASQDO010000050         6236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_251244, whole genome shotgun sequence.
ACCESSION   DASQDO010000050 DASQDO010000000
VERSION     DASQDO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251244"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VEV43_01665"
     CDS             <1..477
                     /locus_tag="VEV43_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYP22255.1"
                     /translation="LKSGRYSIEEPLAIGAALAGAPDDFVARLSAFGGPLGEAFQLRD
                     DLLGTFGDRSHVGKPVDSDIVEGKRNVLYAFAAEAASGEEHAFLTSRWGGDALTADEI
                     ARLRALLESSGARAATEALLDEQVAVARERLDDLCRAGQERDALEELVYLATARRA"
     gene            516..1526
                     /locus_tag="VEV43_01670"
     CDS             516..1526
                     /locus_tag="VEV43_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYP22256.1"
                     /translation="MDPEQIYQEVLSEEQGKGSPSAVAEARAKAARQRARHGSPHPKE
                     PRWWPGSQPQFEGGGDGAAPAPEAEAEPEPVAEEAPAEEPAATPPDIGDASQSERPDA
                     PAPAPAAGTETTAPAPQPAAAPAAAAAVATAPAAAKPAAQARPAGVTHGTDSGNRLRP
                     EDSVGTDAQFDAQKAMADRRRLIDELVSTGVPAATAANAGRPKAPMLLLAYVLIPLIA
                     IGLLAASDSGEAEAEPGGEAPAEQPAGPTERVVAESLQFDTETITLVANEPNEIEFEN
                     ADSAPHNIAIYQDEGAGEAIFEGEQISASTVTYEIEGPEPGEYYFQCDVHPDMNGTAV
                     SE"
     gene            1641..2873
                     /locus_tag="VEV43_01675"
     CDS             1641..2873
                     /locus_tag="VEV43_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HYP22257.1"
                     /translation="MSAEVDVAIVGGGPSGAAAAHYLASRGHSVLVCEKKSFPREKTC
                     GDGLTPRAVKVLEEMGLGDQLQGWERVQGLRVHAAGRTLELPFPELDDFKSFGLVKPR
                     RELDQIVLDNAEAAGAKVLYKTQVHEPIFERGILAGFRAKRDGLDEEVRAKFVICAEG
                     AATKFARSLGRERNLEYPMGLAIRQYFKSPMQSSGWFEAYLDVRSGKDNLPGYGWVFP
                     VGDGTVNAGVGLLSTFGGWRDINLHDLQKNFVGNLPEEWEVNYETVCSKPRAGRLFMG
                     GSVWPPHGPGFLLTGDAAGMINPCNGEGIAYGYETGRIAGRHLDEALRNGSNPVLPGY
                     TQELRETYGPYFRLGRRFVKLIGHPVLMERLVSLGMHSKTVMSFALTLMANLEDSRAH
                     NAEQKGLKAMLKLAEWKP"
     gene            2918..3235
                     /gene="ndhC"
                     /locus_tag="VEV43_01680"
     CDS             2918..3235
                     /gene="ndhC"
                     /locus_tag="VEV43_01680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYP22258.1"
                     /translation="MLFAIASIVAASKLSPRKWTRAKLLPYESGIIPEEGDDKEKFPV
                     KFYLVAMLFIIFDIETVFLYPWAVAYDELQLFGLIEMLAFMVILFAAYAYVWKRGGLE
                     WAE"
     gene            3223..3675
                     /locus_tag="VEV43_01685"
                     /pseudo
     CDS             3223..3675
                     /locus_tag="VEV43_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016555.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            3714..4229
                     /locus_tag="VEV43_01690"
     CDS             3714..4229
                     /locus_tag="VEV43_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYP22259.1"
                     /translation="MAEWVAAADWPEKVASLTGDGWWLADLCGLDTIGLYGGAEDNDP
                     AKRFEVVTQWLHRERKERVTIHVPAEGEPPTVPSIVGTWPEAAFMEREAYDLMGVVFE
                     GHEGLSRLLMPDDWEGHPLRKDYGVGKIPIQWIPQPVMQVDAPGQAPDSGDARQEVDH
                     LGQARPKAETS"
     gene            4226..5461
                     /gene="nuoD"
                     /locus_tag="VEV43_01695"
     CDS             4226..5461
                     /gene="nuoD"
                     /locus_tag="VEV43_01695"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HYP22260.1"
                     /translation="MSVPETVLEALEERGHGAYVESAEDDHMILNMGPVHPATHGVLR
                     LLLELDGEIVVRCQPVIGYLHTGIEKECEDNNWRGAVTCVTRMDYLSQLFNEQAYCMA
                     VEELLGVEVPPRGKWIRTMMVELNRIQSHLVWFGTQGLDMGAISAMFYGFREREVITD
                     FFEMTTGYRMNNSWFVPGGTWDDLPDGWDRAVRSIVRMMPARIDEYEELLSTNPIFLE
                     RTRGVGAISRDDALQVGATGPIARAAGVDWDLRRDMPYEAYGELDFEVPVRSDGDVYA
                     RYEVRIEEMRQSVRLVEQLLDGMPAGDHKNMDPKLTPPPRAELNRSMEAVIHHFKLMT
                     QGFTVPPGEAYVAVESSRGELGAYVVSDGGTAPYRVHVRDPSFVNVQSIPVMAEGGLV
                     ADLIAVIASVDPVMGGVDR"
     gene            5458..6129
                     /locus_tag="VEV43_01700"
     CDS             5458..6129
                     /locus_tag="VEV43_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HYP22261.1"
                     /translation="MTTEPQVLVGEVRETAQRIIDKYPNSRSATLPLLFLVQSVEGYV
                     TEQGMREVADMLGLTSAQVLSSGSFYTMLKKHPHGDYLISVCRNISCTHRGARKVVQA
                     FEERLGVGVGGTTPDGRCTLETAECLATCDGAPAVQVNYEDFYDVKPEDVGEIVDRIE
                     RGALTSSRGEAVKTHKEISFETATVGLRSPGTAGDHTGRTLGGEALRPDTSPGIRRPE
                     LGGGA"
BASE COUNT         1006 a         2162 c         2183 g          885 t
ORIGIN      
        1 ctgaagtcag gccgctactc gatcgaagag ccgctcgcga tcggggccgc gctcgccggc
       61 gcgcccgacg acttcgtcgc gcggctctcg gcgttcggcg gccccctcgg cgaggcgttt
      121 cagctgcgcg acgacctgct cgggacgttc ggcgaccgct cgcacgtcgg caagccggtg
      181 gactccgaca tagtcgaggg caagcgcaac gtcctctacg cgttcgccgc ggaggcggcc
      241 tccggcgagg agcatgcgtt cctcacctct cgctggggtg gcgacgcgct cacggccgac
      301 gagatagccc ggctgcgcgc gctcctcgag tcgtcggggg cgcgcgcggc gaccgaggcc
      361 ctcctggacg agcaggtcgc ggtcgcccgg gagcgcctcg acgacctctg ccgggccggg
      421 caggagcggg acgcgctcga agagctcgtg tatctcgcga ccgcgcgccg cgcctgacgc
      481 ccgaccccgt ctcggtgggg ttagatttac gcctcgtgga ccccgaacag atctaccagg
      541 aagtcctgtc ggaggagcag gggaagggga gcccctcggc cgtcgcggag gcgcgggcca
      601 aggccgcacg ccagcgcgca cgccacggat ccccacaccc gaaggagccc cgctggtggc
      661 ccggctcgca gccccagttc gagggcggcg gcgacggtgc cgcaccggct cccgaggcag
      721 aggccgaacc ggagcccgta gccgaagagg cgcccgccga agagcccgcg gccacgccgc
      781 ccgacatcgg cgacgcgtcc cagtccgagc gtcccgacgc tccggccccc gcgcccgccg
      841 cagggaccga gaccaccgcc cccgcaccac agcccgcggc cgcccctgcg gctgctgccg
      901 cggtcgcgac ggcacccgcc gccgcgaagc cggccgccca ggcccggcct gccggtgtca
      961 cccacgggac cgacagcggc aacaggctgc gccccgagga ctccgtcggg accgacgcgc
     1021 agttcgacgc ccagaaggcg atggcggacc gccgccggct gatcgacgag ctcgtctcca
     1081 cgggcgtccc cgcggccacg gcggccaacg ccgggcggcc gaaggccccg atgctcctgc
     1141 tcgcctacgt gctgatcccc ctgatcgcga tcggcctcct cgctgcgagc gacagcggcg
     1201 aggcggaggc cgagccgggt ggcgaggcgc cggccgagca gcccgcaggg ccgaccgaga
     1261 gggtggtcgc cgagagcctc cagttcgaca ccgagacgat cacgctcgtc gccaacgagc
     1321 cgaacgagat cgagttcgag aacgccgact cggcccccca caacatcgcg atctaccagg
     1381 acgagggggc cggcgaggcg atcttcgagg gcgagcagat ctcggcgagc acggtgacgt
     1441 acgagatcga gggccccgag ccgggcgagt actacttcca gtgcgacgtc cacccggaca
     1501 tgaacgggac cgccgtctcc gaatagcggg gttcccggtt ctcccgtacc gcctgcgggg
     1561 atagactcgg aggcacgctc gtgaaaattt tcacaagcgc ccgggggcgg gtacggcctg
     1621 ccgcataggg aggaggagcc gtgtcggccg aggtcgacgt cgcaatcgtg ggagggggcc
     1681 cttcgggggc cgcagccgcc cactacctcg cctcccgcgg ccactcggtc ctggtctgcg
     1741 agaagaagag cttcccccgc gagaagacgt gcggggacgg cctcacgccc cgggcggtca
     1801 aggtcctcga agagatgggc ctcggcgacc agctccaggg ctgggagcgg gtccagggcc
     1861 tgcgggtcca cgccgccggc cgtacgctcg agctcccgtt ccccgagctc gacgacttca
     1921 agagcttcgg cctcgtcaag ccccgccgcg agctcgacca gatcgtgctc gacaacgccg
     1981 aggccgcggg ggccaaggtc ctctacaaga cccaggtcca cgagccgatc ttcgagcggg
     2041 ggatcctcgc cggcttcaga gcgaagcggg acggtctcga cgaagaggtc cgggccaagt
     2101 tcgtgatctg cgccgagggc gcggccacga agttcgcccg ctcgctcggc cgcgagcgca
     2161 acctcgagta ccccatgggc ctcgcgatcc gtcagtactt caagtcgccg atgcagagct
     2221 ccggctggtt cgaggcctac ctcgacgtgc gctccggcaa ggacaatctc ccgggctacg
     2281 gctgggtctt cccggtgggc gacgggaccg tcaatgccgg cgtcgggctg ctgtccacgt
     2341 tcggcggctg gcgcgacatc aacctccacg acctccagaa gaacttcgtc ggcaatctcc
     2401 cggaggagtg ggaggtcaac tacgagacgg tgtgcagcaa gccgcgtgcc ggccggctct
     2461 tcatgggcgg cagcgtgtgg cccccgcacg gccccggctt ccttctcacc ggcgacgccg
     2521 ccgggatgat caacccctgc aacggggagg gcatcgcgta cggctacgag accggccgga
     2581 tcgcaggacg ccacctcgac gaagcgcttc gcaacgggag caatccggtg ctgcccggct
     2641 acacgcagga gctgcgcgag acgtacgggc cgtacttccg gctcggccgc cgcttcgtca
     2701 agctcatcgg ccatccggtg ctgatggaga ggctcgtctc gctcggcatg cactcgaaga
     2761 ccgtcatgag cttcgcgctg actttgatgg ccaacctcga ggattcccgc gcccataatg
     2821 cagagcaaaa aggcctgaag gcgatgctca agcttgcgga gtggaaaccg tgacgcttcc
     2881 tgcttaccta ccgatcctgt tcctcgtcat cctctcgatg ctgttcgcca tcgcatcgat
     2941 cgtcgccgcg tcgaagctct cgccgcgcaa gtggacgcgc gccaagctgc tgccgtacga
     3001 gagcgggatc atccccgagg agggcgacga caaggagaag ttccccgtca agttctatct
     3061 cgtcgcgatg ctgttcatca tcttcgacat cgagaccgtc ttcctctacc cgtgggcggt
     3121 cgcgtacgac gagctccagc tgttcgggct gatcgagatg ctcgcgttca tggtgatcct
     3181 gttcgccgca tacgcgtacg tgtggaagcg ggggggcctc gaatgggcgg agtgagcgac
     3241 accctcggca cgaacttcgt cacgacgcag ctcgacaagc tcgtgaactg ggcccgcaag
     3301 tcgtcgctgt ggccggcgac gttcggcctc gcctgctgcg cgatcgagat gatggccacg
     3361 acgatgccgc ggcacgacct cgcgcgcttc gggatggagg ctttccgagg ctccccgcgc
     3421 cagtcggacc tgatgatcgt cgcgggccgg gtctctcaga agatggcccc ggtcctgcgt
     3481 cacatctacg accagatgac cgagccgaag tgggtcatct cgatgggcgt gtgcgcgtcg
     3541 agcggcggcg tcttcaacaa ctatgcgctc gtgcagggcg tcgaccagat cgtccccgtc
     3601 gacatctacg tcccgggctg cccgccgcgg cccgagatgc tgatggacgg gatcatgctg
     3661 ctgcacgaca agatctcgcg cggcgagtac accgccggga ggcaagcggt ctgatggcgg
     3721 aatgggtggc cgcggccgac tggccggaga aggtcgcgtc cctgaccggg gacggttggt
     3781 ggctggccga cctgtgcggg ctcgacacca tcggcctcta cggcggcgca gaggacaacg
     3841 acccggccaa gcgcttcgag gtcgtcaccc agtggctcca ccgcgagcgc aaggagcgcg
     3901 tgacgatcca cgtcccggcc gagggcgagc ccccgacggt cccgtcgatc gtcggcacgt
     3961 ggcccgaggc ggcgttcatg gagcgcgagg cctacgacct gatgggcgtc gtcttcgaag
     4021 gacacgaggg cctctcccgg ctgctgatgc cggacgactg ggagggccac ccgctgcgca
     4081 aggactacgg cgtcggcaag atcccgatcc agtggatccc gcagccggtc atgcaggtcg
     4141 acgcgccggg ccaggccccc gactcgggcg acgcacggca ggaggtcgac cacctgggac
     4201 aggcgcggcc gaaggcggag acgtcgtgag cgtgcccgag accgtcctcg aagcactcga
     4261 agaacgcggg cacggcgcgt acgtcgagtc cgccgaggac gaccacatga tcctcaacat
     4321 ggggccggtc caccccgcga cgcacggcgt cctccggctg ctgctcgagc tcgacggcga
     4381 gatcgtcgtc cggtgtcagc ccgtcatcgg ctacctccac accggcatcg agaaggagtg
     4441 cgaggacaac aactggcgcg gggccgtgac gtgcgtgacg cgcatggact acctgtcgca
     4501 gctcttcaac gagcaggcgt actgcatggc ggtcgaggag ctgctcggtg tcgaggtccc
     4561 gccgcgcggc aagtggatcc gcacgatgat ggtcgagctc aaccggatcc agagccacct
     4621 cgtgtggttc gggacgcagg gcctcgacat gggcgcgatc tccgcgatgt tctacggctt
     4681 ccgcgagcgc gaggtcatca cggacttctt cgagatgacg accggctacc gcatgaacaa
     4741 cagctggttc gtgccggggg gcacgtggga cgacctcccc gacggatggg accgtgccgt
     4801 gcggtcgatc gtgcggatga tgccggcgcg catcgacgag tacgaggagc tgttgtcgac
     4861 gaacccgatc ttcctcgagc ggacgcgcgg cgtcggcgcc atctcgcgcg acgacgctct
     4921 ccaggtcgga gcgacgggcc cgatagcgcg ggccgccggc gtcgattggg acctgaggcg
     4981 ggacatgccg tacgaggctt acggcgagct ggacttcgag gtgccggtgc ggtccgacgg
     5041 cgacgtctac gcgcgctacg aggtccggat cgaggagatg cgtcagtcgg tgcggctcgt
     5101 cgagcagctg ctcgacggga tgcccgccgg cgaccacaag aacatggacc cgaagctcac
     5161 gcccccgccg cgggccgagc tgaaccgttc gatggaggcc gtcatccacc acttcaagct
     5221 gatgacgcag ggcttcactg tcccgcccgg agaggcgtac gtcgcggtcg agtcgtcacg
     5281 cggcgagctc ggtgcatacg tcgtctcgga cggcggcacc gcgccgtacc gcgtccacgt
     5341 gcgggacccg tcgttcgtga acgtgcagtc gatcccggtc atggcggagg gcggcctcgt
     5401 cgccgacctg atcgcggtca tcgcgtccgt agaccccgtg atggggggag tggatcgatg
     5461 actaccgagc cgcaggtgct ggtgggcgag gtccgcgaga ccgcgcagcg gatcatcgac
     5521 aagtacccga actcgcgctc ggcgacgttg ccgctgctgt tcctcgtcca gtcggtcgag
     5581 ggctacgtga ccgagcaggg gatgcgcgag gtcgcggaca tgctcggcct cacgtcggcg
     5641 caggtgctct cgtccggcag cttctacacg atgctgaaga agcatcccca cggcgactac
     5701 ctgatctcgg tgtgcaggaa catctcgtgc acccaccgcg gtgcgcgcaa ggtcgtgcag
     5761 gcgttcgagg aacggctcgg cgtcggggtc ggcggcacta cgccggacgg ccgctgcacg
     5821 ctcgagaccg ccgagtgcct cgccacttgc gacggcgcgc ccgcggtgca ggtcaactac
     5881 gaggacttct acgacgtgaa gcccgaggac gtcggcgaga tcgtcgaccg gatcgagcgc
     5941 ggcgcgctca cgtcgtcccg cggcgaggcc gtcaagacgc acaaggagat ctcgttcgag
     6001 accgcgaccg tcgggctgcg ttcgccgggg accgcgggcg accacaccgg acggacgctc
     6061 ggcggagaag ccctgcgccc ggacacgtcg cccggcatcc gccgccccga gctcgggggc
     6121 ggcgcatgag caacgactac gtcgtgaccg ccgggggtga gacgcggatc gtcacgcggc
     6181 gcctgcggga gttccccgcg gactcctgga cgatcgaccg cgtgctcgac gacggc
//
LOCUS       DASQDO010000051         4922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_618393, whole genome shotgun sequence.
ACCESSION   DASQDO010000051 DASQDO010000000
VERSION     DASQDO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618393"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VEV43_01705"
     CDS             complement(<1..766)
                     /locus_tag="VEV43_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013128709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP22262.1"
                     /translation="MPTISMREVTKTFPGSAAPAVTALSLDVEAGETVVLVGPSGCGK
                     TTTMKMINRLEEPTSGTILVGGTDVMQQDPVELRRGIGYVIQSIGLMPHRTVAENVAT
                     VPKLVGWDDARASARVRELAEMLELDRDLLDRYPNALSGGQRQRVGVARALAVDPPVM
                     LMDEPFGAVDPIVRERLQDQFLAIQAELRKTIVFVTHDVDEALKMADRIAILNKGGIV
                     EQFAAPAEILRAPANDFVRRFIGGERALKRLALLSVA"
     gene            complement(774..1310)
                     /locus_tag="VEV43_01710"
     CDS             complement(774..1310)
                     /locus_tag="VEV43_01710"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HYP22263.1"
                     /translation="MPADPWTDPLTAPLPSELERLGSVSSRARTDMFPPSEQTLREER
                     TQQPPQVSKPRRRRVPVRRVKRQLKHVDPLTVLKLSGFYWAVFLVVWLVIVAILYSII
                     EAAGFFDSLEKLNNGFALGWGKLDITLGLVEKWAFLVGITFALITTLVNVFLAFLYNV
                     AADLIGGLELTFVERDTQ"
     gene            complement(1491..4016)
                     /gene="gyrA"
                     /locus_tag="VEV43_01715"
     CDS             complement(1491..4016)
                     /gene="gyrA"
                     /locus_tag="VEV43_01715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HYP22264.1"
                     /translation="MPDQPRFPGPEDPSISDITIEEEVQYSFLEYAMSVIVSRALPDV
                     RDGLKPVHRRILYAALEAGLRPDRPFRKCAALVGDVMKKYHPHGDQSIYDALARLAQD
                     FSTRNLLIDGQGNFGSIDGDAPAAMRYTECRLSKLSMELVRDIDEETVDFEPNYDGYE
                     IQPKVLPARFPNLLVNGSGGIAVGMATNIPPHNLGEVVDAVVEVLENPELGMDKEKML
                     PALMKHIKGPDFPTGATILGRQGIVDAYTTGRGSIKMRAVCEVEEGPRGNPRIIVTEI
                     PYQVNKARLAQKIAELVNAKENRLEGIADLRDESSERGGMRLVIDLKKSAVPQVVLNT
                     LYKRTQLQDNFGVNMLALVDGVPRTLNLAEVVEAYITHQIDVVVRRTRYRLRKAEERS
                     HILEGLIIALDNIDEIVALIRAAADTAEARDGLMARFGLTEIQANHILDMPLRRLTAL
                     EQDKIREEHAELQKTIADLRDILANPGRVREIIATELKDVRKRFADPRRSKLVPDEGE
                     FDIEDLIADEDLVVSVSRDGYVKSVPLDTYRKQGRGGRGVKGAALKEGDIVEHLLTTT
                     AHAYLLMFSSNGRVYRVKAHELPKRDRTARGTAVVNVVPMRPEDKVAAVIDTRDYETF
                     RYLLIATKGGIVKKTKFSEYDSSRREGIIAVNLKDGDEVVRVRPTSGNDDVLVITKKG
                     KAIRFSESAVRSMGRTATGVIGIRLDEDDEVVSCAVVSGEETKGELLVVTQFGYGKRT
                     KLSEFPRKGRGGKGVIAAKLTKPRGPLVGAVIVNSDDEVFLISDDGQVIRMPVKTISR
                     QGRPATGVRVMNLSGGTSVSAVAPVVNEPDDGPAPPDQPKLGE"
     gene            complement(4023..>4922)
                     /locus_tag="VEV43_01720"
     CDS             complement(4023..>4922)
                     /locus_tag="VEV43_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HYP22265.1"
                     /translation="QTKTKLGNTEIRSFVETSMNQRFAEFMEEHPSEARAICNKAVSA
                     QRARLEARKARDLVRRKSLLESSTLPGKLADCQTRDPEQAELLIVEGDSAGGSAKQAR
                     QREFQAILPLRGKILNVERARLAKILENKEIQAIITAIGTGIGDEFDMTKCRYHKIVS
                     MADADVDGAHIKSLLLTFFFRYMRDLIDAGYVYVAQPPLYRVPYKGKVHYFKNDEQLE
                     AFKEANNGSKIEASRFKGLGEMNPSELWETTLDPERRTLLRVTMEDAALAEEMFSTLM
                     GEDVEARKGFIQRNAKDVRFLDI"
BASE COUNT          726 a         1600 c         1700 g          896 t
ORIGIN      
        1 cggccacgct cagcagcgcc agccgcttca gcgcccgctc gccgccgatg aagcggcgga
       61 cgaagtcgtt cgcgggggcg cgcaggatct ccgcgggggc cgcgaactgc tcgacgatcc
      121 cgcccttgtt caggatcgcg atgcggtcgg ccatcttcaa cgcctcgtcg acgtcgtgcg
      181 tgacgaacac gatcgtcttg cggagctcgg cctggatcgc gaggaactgg tcctggagac
      241 gctcgcgcac gatcgggtcg acggccccga acggctcgtc catcagcatc acgggagggt
      301 cgacggcgag cgcgcgggcg acccccaccc tctgccgctg tccgcccgac aacgcgttcg
      361 gatacctgtc gaggaggtcg cggtcgagct cgagcatctc ggcgagctcg cgcacgcgcg
      421 ccgacgcgcg cgcgtcgtcc cacccgacga gctttggcac cgtcgcgacg ttctccgcca
      481 ccgtccggtg cggcatgagg ccgatgctct ggatgacgta gccgatgccg cggcgcagct
      541 cgaccgggtc ctgctgcatg acgtcggtcc cgccgacgag gatcgtgccg gaggtcggct
      601 cctcgagacg gttgatcatc ttcatcgtcg tcgtcttgcc gcagcccgac ggtccgacga
      661 ggacgacggt ctccccggcc tcgacgtcga gcgagagcgc ggtgacggcg ggcgcggccg
      721 agccgggaaa ggtcttggtg acctcacgca tcgagatcgt gggcatgtgc tcgctactgt
      781 gtgtcgcgct cgacgaacgt gagctcgaga cccccgatca ggtcggccgc cacgttgtag
      841 aggaacgcga ggaagacgtt cacgagcgtg gtgatcaacg cgaacgtgat cccgaccagg
      901 aacgcccact tctcgacgag gccgagcgtg atgtcgagct tgccccaccc cagagcgaac
      961 ccgttgttca gcttctcgag gctgtcgaag aagccggcgg cctcgatgat cgagtacagg
     1021 atcgcgacga tgacgagcca cacgacgagg aagaccgccc agtagaaacc cgacagcttc
     1081 agcaccgtca gcgggtcgac gtgcttcagc tgacgcttga cgcggcgtac ggggacgcgg
     1141 cgccggcgcg gcttcgatac ctgaggcggt tgctgagtac gttcctctcg cagcgtctgc
     1201 tcggacgggg ggaacatatc ggtgcgcgca cgcgacgaca ccgagcccag ccgctcgagc
     1261 tcggagggga gtggagccgt cagcgggtcg gtccaggggt cggcgggcac ggtttcgggc
     1321 gtgaggtgac ctcccgaagc ggtagccgct tcgggattcg cgtgccagcc tctctcgcgc
     1381 tcttccctag aggacaaagc tctcccccgt tcggaaaaca gccttcagta tgggctattg
     1441 gactcggcgt cgccggatgg agttcgaatc gcggcgcacg aacacgaacg tcactcgccg
     1501 agcttcggct ggtcgggagg ggcggggccg tcgtccggct cgttcacgac cggcgcaact
     1561 gccgacaccg aggtgccgcc cgagaggttc atcacgcgca cgcccgtcgc gggacggccc
     1621 tgacgcgaga tggtcttgac cggcatgcgg atgacctggc cgtcgtccga gatgaggaag
     1681 acctcgtcgt ccgagttgac gatgaccgcg ccgactaggg ggccgcgcgg tttcgtgagc
     1741 tttgcggcga ttacgccctt gcccccgcgg cccttgcgcg ggaactccga gagcttcgtg
     1801 cgcttgccgt agccgaactg ggtcacgacg aggagctcgc ccttcgtctc ctcacccgac
     1861 accacggcgc acgagacgac ctcgtcgtcc tcgtcgaggc ggatgccgat gacgccggtg
     1921 gccgtacgcc ccatcgagcg cacggcggac tcggagaagc ggatggcctt gcccttcttc
     1981 gtgatcacga ggacgtcgtc gttgccggac gtgggccgga ccctgacgac ctcgtcgccg
     2041 tctttgaggt tgacggcgat gatcccctcg cggcgggagg agtcgtactc gctgaacttc
     2101 gtcttcttga cgatgccgcc cttggtcgcg atcagcagat agcggaacgt ctcgtagtcg
     2161 cgggtgtcga tgaccgcggc gaccttgtcc tcgggacgca tcgggacgac gttgacgacc
     2221 gcggtgccgc gcgcggtgcg gtcgcgcttg gggagctcgt gcgccttcac gcggtagacg
     2281 cgcccgttgc tcgagaacat cagcaggtac gcgtgcgcgg tcgtcgtgag gaggtgctcg
     2341 acgatgtcgc cttccttgag agccgcgccc ttgaccccgc ggcccccgcg gccctgtttc
     2401 cggtaggtgt cgagcgggac gcttttcacg tagccgtcgc gcgagaccga gacgacgagg
     2461 tcctcgtcgg cgatcaggtc ctcgatgtcg aactcgccct cgtcgggcac gagcttcgag
     2521 cggcgggggt cggcgaaccg cttgcggacg tccttgagct cggtcgcgat gatctcgcgc
     2581 acgcggcccg ggttcgcgag gatgtcgcgc aggtcggcga tcgtcttctg gagctcggca
     2641 tgctcctcgc ggatcttgtc ctgctcgagc gcggtgagcc ggcgcagcgg catgtccagg
     2701 atgtggttcg cctggatctc ggtcagcccg aagcgggcca tgagcccgtc gcgtgcctcg
     2761 gcggtgtcgg cggccgcccg gatcagcgcc acgatctcgt cgatgttgtc gagggcgatg
     2821 atgagccctt cgaggatgtg cgaccgctcc tcggccttgc gcagccggta gcgcgtgcga
     2881 cgcacgacga cgtcgatctg gtgcgtgatg tacgcctcga cgacctcggc gaggttcagc
     2941 gtgcgaggga cgccgtcgac gagagccagc atgttcacgc cgaagttgtc ctggagctgt
     3001 gtgcgcttgt agagcgtgtt gaggacgacc tgcggcactg cgctcttctt caggtcgatc
     3061 acgaggcgca tgccgccgcg ctcgctcgac tcgtcgcgca ggtcggcgat gccctcgagc
     3121 cggttctcct tcgcgttgac gagctcggcg atcttctgcg cgagccgcgc tttgttcacc
     3181 tggtagggga tctccgtcac gatgatccgc gggttcccgc gcggcccctc ttcgacctcg
     3241 cagaccgcgc gcatcttgat cgagccgcgg ccggtcgtgt acgcgtcgac gatcccctga
     3301 cgcccgagga tcgtcgcgcc cgtcggaaag tccggcccct tgatgtgctt catgagggcc
     3361 ggaagcatct tctccttgtc catcccgagc tcggggttct cgaggacctc gacgacggcg
     3421 tcgacgacct cgccgaggtt gtggggcggg atgttggtgg ccatgccgac ggcgatgccc
     3481 ccggagccgt tgacgaggag gttggggaac cgggcgggga ggaccttggg ctggatctcg
     3541 tagccgtcgt agttgggctc gaagtcgacg gtctcctcgt cgatgtcgcg gacgagctcc
     3601 atggagagct tggagagccg acactcggtg tagcgcattg cggccggagc gtcgccgtct
     3661 atcgagccga agttgccctg cccgtctatc agcaggttcc gcgtcgagaa gtcttgcgcc
     3721 aggcgggcca gcgcgtcgta gatcgactgg tcgccgtgcg ggtggtactt cttcatcacg
     3781 tcgcctacga gggccgcgca cttgcggaac gggcggtcgg ggcgcaggcc ggcttcgagg
     3841 gcggcgtaca ggatccggcg gtgcaccggc ttcagcccgt cgcgcacgtc cggcaacgcg
     3901 cgcgacacga tgacggacat cgcgtactcg aggaacgagt actggacctc ttcctcgatc
     3961 gttatgtcgg agatcgaggg atcctcggga ccggggaatc taggttgatc aggcatggcc
     4021 tcttagatgt ctaggaaacg cacgtctttg gcgtttcgct ggatgaatcc cttgcgggcc
     4081 tcgacgtcct cgcccatcag cgtcgagaac atctcctcgg cgagagcggc gtcttccatc
     4141 gtcacgcgca gcagcgtcct gcgctcgggg tcgagcgtgg tctcccacag ctcggacggg
     4201 ttcatctcgc cgaggccctt gaagcgcgag gcctcgatct tggagccgtt gttcgcttcc
     4261 ttgaacgcct cgagctgctc gtcgttcttg aagtagtgca ccttgccctt gtagggcacc
     4321 cggtagagcg gcggctgcgc gacgtacacg tagccggcgt cgatcaggtc gcgcatgtaa
     4381 cggaagaaga acgtgagcag caggctcttg atgtgcgcgc cgtcgacgtc cgcatcggcc
     4441 atcgacacga tcttgtggta gcggcacttc gtcatgtcga actcgtcgcc gatcccggtc
     4501 ccgatcgcgg tgatgatcgc ctggatctcc ttgttctcga ggatcttggc gagccgcgcc
     4561 cgctcgacgt tcaggatctt gccgcgcagc gggaggatcg cctggaactc gcgctggcgc
     4621 gcctgcttcg cgctgccccc ggccgagtcg ccctcgacga tcaacagctc cgcctgctcg
     4681 ggatcgcgag tctggcagtc ggcgagcttg cccggcagcg tcgacgactc gagcagcgac
     4741 ttgcgccgca cgaggtcgcg cgccttgcgc gcttcgagac gagctctctg cgcggagacg
     4801 gccttgttgc agatcgcgcg ggcctccgac gggtgctcct ccatgaactc ggcgaagcgc
     4861 tggttcatcg aggtctcgac gaaggagcgg atctcggtgt tgccgagctt ggtcttggtc
     4921 tg
//
LOCUS       DASQDO010000052         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_740942, whole genome shotgun sequence.
ACCESSION   DASQDO010000052 DASQDO010000000
VERSION     DASQDO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740942"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VEV43_01725"
     CDS             complement(<1..463)
                     /locus_tag="VEV43_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP22266.1"
                     /translation="MTRPVCASSWTAAPSSRRTVVFVAGFADGLPRSLVARLARAGLT
                     RVVPLRTIDAATVEGPAAAIEEIASWRVTRYVEPDDRASFLNYQTSEQTGEGAAKAGR
                     RPLPHGLSGKGVTVAVVDSGVDTHHPDLRDRVIGRLTFEATWAANGVVGPEQ"
     gene            complement(577..1797)
                     /locus_tag="VEV43_01730"
     CDS             complement(577..1797)
                     /locus_tag="VEV43_01730"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HYP22267.1"
                     /translation="MALREVSLDEVRRLAIRAQRLDRAVAPSRETILDLVRDLGCVQI
                     DPISVVAPTQHLVLWSRLGAFDRADLDALLWEDRSLFHYWAHAASIVPTSDYPIHHLM
                     MRRWGKGTAKYEERVREWMRVNQSMRRKVVSEIRRRGPLCISDLRDITGERWKSSGWT
                     DEQGVARMIEFLWAKGVVTIAGRAGAARLWDLMDRWLPEWTPRETWPERRVVEESVLR
                     SLRSLGVATPRQIKEHFTRYRYPGLSDALARLVRRGLVEEVAVVGGGERLKGRWYVRA
                     EDAALLDGSLEEASFCRLLSPFDNLICDRARTELVWGFEYRIEIYVPKDKRRWGYYVL
                     PILWGDRLVGRIDSAVDRGAGVLRVLSVHSEPRAPAIGDEVAGAVRDLAGFVGASAVE
                     WPRAKPRIWGKSLT"
     gene            complement(1843..2229)
                     /locus_tag="VEV43_01735"
     CDS             complement(1843..2229)
                     /locus_tag="VEV43_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22268.1"
                     /translation="MATRQAPPPPPPPVASRHGAALEEVAAGQRYLIFAILVHIGALV
                     ARAASDGNWLVFAPIAIAAAALAVVGLLRLAGGLGLSSTTIALLFVVALVPVLAFVML
                     AMVNARATKALRAGGYEVGFFGARKP"
     gene            complement(2288..>3359)
                     /locus_tag="VEV43_01740"
     CDS             complement(2288..>3359)
                     /locus_tag="VEV43_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HYP22269.1"
                     /translation="RRSAAFGEAWAELEPELRRSLASAPANVVDDVLQETSIKLLRVW
                     DSIDHDRPALPLAVTIARNALRDELRRSARHSHAELWEEARVRDDLDTVVSARLELAR
                     VGSELTRLTPGQRAALLAEVGGGPRPADTPRIKMLRSRARARLRELVAQAGAFVTTGV
                     ARLRTSAGRAFSGLPDVGILAQAVLLAVVATGSAETMQERDLLDPGRRVVVAVGETGR
                     REAAAPVAGSSERAGGGVTPTAQGTGVPLEPRVVRSDGDGPSQPGLPTPPIPRSHGNA
                     TDGGYFGTEGWTQAGGAETSAAGHDAGYRYETEWESPGCVQRLAEGEAPGRCDAPEEP
                     SAAAEVEVDGRRHRVDTDDRPR"
BASE COUNT          489 a         1268 c         1176 g          426 t
ORIGIN      
        1 gctgctccgg ccccacgacg ccgttggcag cccacgtcgc ctcgaacgtc agacgtccga
       61 tcacgcgatc ccgcaggtcc gggtggtgcg tgtcgacacc ggagtcgacg acggcgaccg
      121 tcacgccctt gcccgacagc ccgtgcggca gcggacggcg tccggccttg gccgcgcctt
      181 cgcccgtctg ctcggacgtc tggtagttga ggaagctagc gcggtcgtcg ggctcgacgt
      241 agcgcgtgac ccgccacgac gcgatctctt cgatcgcggc ggcggggccc tcgacggtcg
      301 cggcgtcgat cgtgcggagc ggaacgactc gcgtgagccc cgcgcgggcg aggcgcgcga
      361 cgagcgaccg gggcagaccg tcggcgaagc ccgcgacgaa aacgaccgtt cggcgactcg
      421 acggagcggc cgtccacgac gacgcgcaca cagggcgcgt cacagcggtc gtctccgaat
      481 gcgggggccg aggcggcggc gagcgcggca aggacgagac gggcaagagc tctcttcatg
      541 ggacgagcgt tcgcccaccc gcgggggatt tcttgcctag gtgagcgact tcccccagat
      601 gcggggcttc gcccggggcc actccaccgc cgaggccccc acgaagcccg cgaggtcacg
      661 cacggcgccc gcaacctcgt ctcctatcgc cggcgcacgc ggctccgagt gcaccgacag
      721 cacccgcagg accccggccc ccctgtcgac ggccgagtcg atccggccca cgagccggtc
      781 cccccacagg atcggcagca cgtagtagcc ccagcgacgc ttgtccttcg ggacgtagat
      841 ctcgatgcgg tactcgaagc cccacacgag ctccgtccgg gcccggtcgc agatcaggtt
      901 gtcgaacggc gagagcaggc ggcagaacga cgcttcttcg agcgagccgt cgagcagcgc
      961 ggcgtcctcg gccctcacgt accagcgacc cttcaatcgt tcgccgccac cgacgaccgc
     1021 gacttcctcg accaggccgc gtcgcacgag ccgcgccagc gcgtcggaga gccccgggta
     1081 gcgataacga gtgaagtgct cctttatctg acgcggcgtc gcgactccca gcgagcgcag
     1141 cgaccgtagg acggactcct cgacgacgcg acgctccggc cacgtctcgc gcggcgtcca
     1201 ctccggaagc caacggtcca tgaggtccca caagcgcgcg gcccccgccc gcccggcgat
     1261 cgtcacgacg cccttcgccc agaggaactc gatcatccgc gcgacgccct gctcgtccgt
     1321 ccagcccgac gacttccacc gctcccccgt gatgtcgcgg aggtcggaga tgcacagggg
     1381 gccgcgccgg cggatctcgg agacgacctt gcgccgcatc gactggttca cgcgcatcca
     1441 ctcgcgcacg cgctcttcgt acttggccgt ccccttgccc cagcgcctca tcatcagatg
     1501 gtggatcgga tagtccgacg tcggcacgat cgacgcggcg tgggcccagt agtggaagag
     1561 cgatcggtcc tcccacagca gggcgtcgag gtcggcgcgg tcgaatgccc cgagcctgct
     1621 ccagagcacc aggtgctgcg tcggcgcgac gaccgagatc gggtcgatct gcacgcagcc
     1681 gaggtcgcgg acgagatcga ggatcgtctc gcgcgacggt gcgaccgcgc gatcgagacg
     1741 ctgtgcccgg atcgcgagcc ggcggacctc gtcgaggctt acctcacgta gcgccatggg
     1801 ggcagaagct accggccgcg ggcccgaccc gcgctgagac ggctagggct tacgagcgcc
     1861 gaagaagccg acctcgtagc cgccggcgcg cagcgccttc gtcgcccgcg cgttgaccat
     1921 cgccagcatc acgaaggcga ggaccgggac cagcgcgacg acgaacagga gcgcgatcgt
     1981 cgtcgaggag agcccgagcc cgcccgcgag ccgcaacagg ccgacgaccg cgagcgcagc
     2041 cgccgctatg gcgatcgggg cgaacacgag ccagttgccg tcggacgcgg cacgggccac
     2101 gagggcgccg atgtggacga ggatcgcgaa gatcaggtac ctctgccccg ccgcgacctc
     2161 ttcgagcgcg gcgccgtggc gcgacgctac gggtggcggc ggtggcggcg gcgcctgtcg
     2221 tgtggccatc acaccctttg tcgatgaccg gcggccccca cttgaggcga tcccggcaaa
     2281 tcgcccgtca gcgcgggcgg tcgtcggtgt ccacccggtg gcgccgtccg tcgacctcga
     2341 cctcggccgc agccgaaggc tcctcggggg cgtcgcaccg gcccggcgcc tcgccctcgg
     2401 cgagcctctg cacgcacccg ggactctccc attcggtctc gtaccggtac ccggcgtcgt
     2461 gcccggccgc cgaggtctcg gcgccgcccg cctgcgtcca gccctcggtg ccgaagtaac
     2521 cgccgtcggt ggcgtttccg tgcgacctcg ggatcggcgg cgtcgggagc ccgggctgcg
     2581 acgggccgtc gccgtcactg cggacgacac ggggctcgag cgggacgccg gtcccttgcg
     2641 ccgtcggggt gaccccgccc ccggctcgct cggacgaccc tgcgacgggc gcggcggcct
     2701 ctcggcggcc ggtctcgccc accgccacga ccacccgccg gcccggatcc aagaggtccc
     2761 gctcctgcat cgtctccgca gagccggtcg ccaccacggc cagaaggacg gcctgggcca
     2821 ggatgccgac gtccggcaat cccgagaagg cccggcccgc cgacgtccgc aagcgtgcga
     2881 caccggtagt gacgaacgct cccgcctgcg cgacgagctc gcggagccgg gcgcgggccc
     2941 gggagcgcag catcttgatc ctcggcgtgt cggccggccg cggcccgccc ccgacctcgg
     3001 ccaggagcgc ggcccgctgg cccggcgtga ggcgcgtgag ctcggatccg acacgcgcca
     3061 gctcgaggcg ggcggacacc acggtgtcga ggtcgtctcg gacccgcgcc tcttcccaga
     3121 gctcggcatg cgagtggcgc gcggacctcc gcagctcgtc gcgcagggcg ttgcgcgcga
     3181 tcgtgaccgc gagcgggagg gccggccggt cgtggtcgat cgagtcccag accctcagca
     3241 gctttatcga cgtctcctgc aagacgtcgt ccacgacgtt ggcgggggcc gaggcgaggc
     3301 tgcgccgcag ctcgggctcg agctcggccc aggcctcgcc gaaggcggcg gaccgcctg
//
LOCUS       DASQDO010000053         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_251490, whole genome shotgun sequence.
ACCESSION   DASQDO010000053 DASQDO010000000
VERSION     DASQDO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251490"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VEV43_01745"
     CDS             <1..676
                     /locus_tag="VEV43_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22270.1"
                     /translation="TNGENAVNLTWRPAETHEDYVDDRAADAAETWDVEIARRHGKLF
                     RYEGTTDFTALWIDGDLSLELRGVFENVHAYRAIARTLDRVDEETWLAALPADTVTPD
                     ERAAVVDEMLSDVHVHPDVDVDALKESATINDRYQLGARVTAAVACEWIEQWLEGNSP
                     EKVKAAGAMAGSREWAILREMRPQGAWTEVIWEYADAMAGDGQVPGGRPMTVVESYRD
                     GLGCPS"
     gene            complement(836..1561)
                     /locus_tag="VEV43_01750"
     CDS             complement(836..1561)
                     /locus_tag="VEV43_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HYP22271.1"
                     /translation="MSTVRPYGDATGDGQVQLSFTLPLADSPRAHQAALELARKMGFA
                     RPQLVHAKPMGPDFTFFILYGATSFDVDPDELEVEERDFPLYSYAEVNQMIREGLKRQ
                     LVVVGACTGTDAHTVGLDAILSMKGFAGDHGLEYFPEMRVVNMGAQVTPDEIVSTVSS
                     ESADAVLISQVVTQRDAHLLHLREVREALEAANLRDDLILVGGGPRFTPQQAGELGYD
                     RIFGRGTLPSEVASYLAWRAVAS"
     gene            complement(1558..>2283)
                     /locus_tag="VEV43_01755"
     CDS             complement(1558..>2283)
                     /locus_tag="VEV43_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HYP22272.1"
                     /translation="QHFSRMVHALAGIGINTGEDNYLTTADAVDAAHTVLASQFLNER
                     LAHAAGLAEEQMGRGHAFEIDPAVPDQIMMELAHAQLVRECFPRHPLKYMPPTKHMTG
                     NVFQGFLSDGMFNLIGALTGQDIILLGMMTEGIHTPFLSDRDLALENASYVFGGASGL
                     AGELRFEEGGAVERRAAQVLEECRQMLRKVSEIGMFEAIEAGMFADTVRTRDGGRGLD
                     GVVRRGDGYFNPIADLLEERVRS"
BASE COUNT          362 a          799 c          777 g          345 t
ORIGIN      
        1 cacgaacggc gagaacgcgg tgaacctcac gtggcggccc gccgagacgc acgaggacta
       61 cgtcgacgac cgcgccgccg acgccgccga gacgtgggac gtcgagatcg cgcggcgtca
      121 cgggaagctc ttccgctacg aagggacgac cgacttcacg gcgctgtgga tcgacggcga
      181 cctctcgctg gagctgcgcg gtgtgttcga gaacgtccac gcctaccgcg ccatcgcccg
      241 gaccctcgac cgggtggacg aagagacgtg gctcgcggcg ctgcccgcgg acacggtcac
      301 tcccgacgag cgagccgcgg tcgtcgacga gatgttgagc gacgtgcacg tgcatcccga
      361 cgtggacgtg gacgccctga aggagagcgc gacgatcaac gaccgctatc agctgggggc
      421 ccgggtgacg gcggcggtcg cgtgcgagtg gatcgagcag tggctggagg gcaactcgcc
      481 cgagaaggtg aaggccgccg gggcgatggc gggctcccgc gagtgggcga tcctccggga
      541 gatgaggccc caaggcgcct ggaccgaggt catctgggag tacgcggacg cgatggccgg
      601 cgacgggcag gtgcccgggg gccggccgat gaccgtcgtc gagtcgtacc gcgacgggct
      661 gggctgcccc tcctagccct cgagccgttt ggcgagatcc gcgacgcgct gggcgatggc
      721 gtcgcggatc tcgccgtagt ccttcagagg ccggcccgcg cgatctcgcg caggtaagcc
      781 tcggcgacgc gcgacttccc ggcgttgtga gcgcagacga acacgacctt cttcttcacg
      841 atgcgacggc cctccaggcc aggtagctcg ccacttcgct cggcaaggtg ccgcggccga
      901 agatgcggtc gtagcccagc tcgccggctt gctgcggcgt gaagcgcggc ccccctccca
      961 ccaggatcag gtcgtctcgc aggttcgccg cctccagggc ttcgcgtact tcgcggaggt
     1021 gcagcaagtg cgcgtccctc tgcgtcacga cctgagagat cagcacggcg tccgccgatt
     1081 ccgacgagac cgtcgagacg atctcgtccg gcgtcacctg ggcccccatg ttcaccacgc
     1141 gcatctccgg gaagtactcg agcccgtgat cgccggcgaa gcccttcatc gacaggatcg
     1201 cgtcgaggcc caccgtgtga gcgtccgtcc ccgtgcacgc gccgacgaca acgagctgcc
     1261 gcttcaggcc ttcacggatc atctggttca cctcggcgta cgagtagagc gggaagtcgc
     1321 gctcctcgac ctcgagctcg tccgggtcga cgtcgaacga cgtggctccg tagaggatga
     1381 agaacgtgaa gtccgggccc atcggcttcg catggacgag ctggggacgg gcgaacccca
     1441 tcttccgggc gagctccaga gccgcttgat gagcgcgcgg cgagtccgcc aagggcagcg
     1501 tgaacgacag ctgtacctgg ccgtcgccgg tggcgtcccc atagggcctg accgtgctca
     1561 cgaacggacc ctctcttcga gcaggtcggc gatcgggttg aagtaaccgt ccccccggcg
     1621 caccacaccg tccaggcccc ggcccccgtc gcgcgttcgc accgtgtcgg cgaacatccc
     1681 cgcctcgatc gcctcgaaca tcccgatctc ggagaccttg cgcagcatct gccggcattc
     1741 ctccagcacc tgcgcggccc tgcgctcgac cgcgccgccc tcttcgaacc gcagctcccc
     1801 cgcgagcccc gaagccccac cgaacacgta cgacgcgttc tccagcgcga ggtcccggtc
     1861 cgacaggaac ggcgtgtgga tcccctcggt catcatcccg agcaggatga tgtcctgccc
     1921 ggtcagcgcc ccgatcaggt tgaacatccc gtcggagagg aagccctgga acacgttgcc
     1981 ggtcatgtgc ttggtcggcg gcatgtactt gagcggatga cgcgggaagc actcgcgcac
     2041 gagctgcgcg tgcgcgagct ccatcatgat ctggtccggc accgccgggt cgatctcgaa
     2101 cgcgtgcccg cggcccatct gctcctctgc gagcccggcc gcgtgcgcga gccgttcgtt
     2161 caggaactgc gacgcgagca cggtgtgggc cgcgtccacc gcgtcggcgg tcgtgaggta
     2221 gttgtcctca ccggtgttga tgccgatgcc ggcgagcgcg tggaccatcc gcgagaagtg
     2281 ctg
//
LOCUS       DASQDO010000054         1845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_129180, whole genome shotgun sequence.
ACCESSION   DASQDO010000054 DASQDO010000000
VERSION     DASQDO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129180"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..960)
                     /locus_tag="VEV43_01760"
     CDS             complement(28..960)
                     /locus_tag="VEV43_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22273.1"
                     /translation="MKLYQPQQRIAKQVLLELTEWPRKLAPAERSYLDGVCAWPIERV
                     DDGPKPVGVTMKRLPDRFFRHLSTGVSRPLELQYLLRPRSCVRLGIEVPSDEQRLTIC
                     TRYAEFLSFLHAHERIYGDVSATNWLWAAAPTPEIFVIDCDATRKRGGVPAVPVGGTP
                     EWMDPTVPSSAQDVHSDRLLLSIAIIRILTGDPRRVYDPSLQQFGPPLTPEIVDLVRR
                     AAVGSRGDRPPAGDYVQALTRALTSPGPASVRRRDTIQLHPKGASTGTTQAPRTRGSI
                     TLGGGRPKPAQPLAAGLWLPLMILLAWLASRAAA"
     gene            complement(1084..>1845)
                     /locus_tag="VEV43_01765"
     CDS             complement(1084..>1845)
                     /locus_tag="VEV43_01765"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HYP22274.1"
                     /translation="MEVRAASVRGVGHRLNGEPRQDAYAIGLSPCGRYIAMAVADGTT
                     AAPRAEMGAWAAVRAAVSLAGKLASEGNTLILAGREDLFHDVAEAIEVVGAVELDLDG
                     PAVARELATTLVVATVATAPPHDAWIGRIGDSTAMILHEGRWERIFPANDGLISTSVT
                     DTLPGQPQDVQQEVVRVGPGSALVLMSDGISGPLGDGKGDVGDLLAQTWAVPPPALVF
                     VRELDFARSTCDDDRTAVVLWAPSADQAGPRAEGR"
BASE COUNT          296 a          640 c          628 g          281 t
ORIGIN      
        1 ccagcaagtt gcggacaggg acactcatca cgcggccgcc cggcttgcaa gccacgccaa
       61 gaggatcata agggggagcc acaggcccgc ggccagtggc tgcgcgggct tcggccgccc
      121 gcccccgagt gtgatcgagc cgcgagtccg aggagcctga gtcgtgcccg tggaggcacc
      181 cttcgggtga agctgaatcg tgtcccggcg gcgcaccgag gcggggccgg gactggtaag
      241 agctcgcgtg agcgcctgga cataatcgcc cgccggcggt cgatccccgc ggctcccgac
      301 ggcggctcgc ctgacgagat cgacgatctc cggggtaagc gggggcccga actgctggag
      361 gctcgggtcg tagacacgac gggggtctcc ggtgaggatg cgaatgatcg cgatcgacag
      421 caacagccgg tccgaatgga cgtcctgcgc cgaggacggg accgtcggat ccatccactc
      481 gggggtgccc cccacgggga ctgcgggaac tccacctctt ttcctcgtcg cgtcgcagtc
      541 gatgacgaag atctccggag tcggcgcagc ggcccatagc cagttcgtag ccgagacgtc
      601 cccgtagatc cgctcgtgcg cgtgtagaaa gctcaggaat tccgcgtacc tcgtgcagat
      661 cgtcaggcgc tgctcgtcgg aggggacctc gatccccagc cgcacgcaag agcgcggccg
      721 gagcaagtac tgaagctcca gcgggcggga taccccggtg ctgaggtgcc ggaagaagcg
      781 gtccggtaag cgcttcatcg tcacgccgac cggcttcgga ccatcgtcga cgcgctcgat
      841 cggccacgcg cagactccgt ccaggtagct tcgctcggcc ggagccagct tgcggggcca
      901 ctcggtgagc tcgaggagca cctgcttggc gattctctgc tgcggctggt aaagcttcac
      961 gacgtgaccc ggacggtccg gtagcgcgaa gacttcgcct tgtccgccgg tcccaccgcc
     1021 ggacgccgag ttaccgagcg gaacgagctg tccgaggtct gccagttgca cgatcggcac
     1081 ccgttatcga ccttccgctc tcggacctgc ttgatcagcc gacggggccc acaggaccac
     1141 cgccgttcgg tcgtcgtcgc aggtgctgcg cgcgaagtcg agctcgcgga cgaacaccag
     1201 cgcaggcggc gggacggccc aggtctgggc cagcaggtcg ccgacgtcgc ccttcccgtc
     1261 cccaagggga cccgagatgc cgtcactcat cagaacgaga gcggagccgg gaccgacccg
     1321 aacgacctct tgctgcacgt cttgcggctg gccgggcagc gtgtcggtga ccgaggtgga
     1381 gatgagcccg tcgtttgcag ggaagatccg ctcccatctc ccctcgtgaa ggatcatcgc
     1441 cgtgctgtcg ccgattcgcc cgatccaagc gtcgtgagga ggagcggtgg cgacggttgc
     1501 tacgacgagt gtggtagcga gctcgcgtgc cacggcgggc ccgtccaggt cgagctcgac
     1561 ggctccaacc acctcgatcg cctcggcgac gtcatggaac aggtcctccc tgccggccag
     1621 gatcagcgtg ttgccctcgc tggcgagctt gcccgccagc gagacggcag cgcgcacggc
     1681 ggcccacgcg cccatctccg ccctcggcgc ggccgtcgtc ccgtcggcga ccgccatcgc
     1741 gatgtatcgc ccacacggcg acaagccgat cgcgtacgcg tcctgccgcg gctcgccgtt
     1801 gagacggtgg cccactcccc tgacgcttgc cgcgcgcact tccat
//
LOCUS       DASQDO010000055        13917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_312588, whole genome shotgun sequence.
ACCESSION   DASQDO010000055 DASQDO010000000
VERSION     DASQDO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312588"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VEV43_01770"
     CDS             <1..1307
                     /locus_tag="VEV43_01770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYP22275.1"
                     /translation="GGAVAATAAGGLDGRAVAHRAGGRAVQVERLPRVETFSIGHNAA
                     EPTLGVTAEGDVFYAAADPTTLVPPLPVDIMRSTDGGQTWEVKSPQLAGNNVHASTGD
                     PYVYVDRTRAGARVFTVDLQGYACSVLSFSDDGGDSWVTNPLGCGRPVNDHQTVFAGP
                     PVESSTLGYPHVVYYCYHDTVTSSCTKSLDGGITFVPTGEPSYTAYTESGEFCGGLHG
                     HGFAGPDGTVYIPKGHCGVPVLAISKDEGASWTRVEVAKLPSAGHEASVAADKRGNVF
                     YAYIGIDMLPYLVVSRDGGSTWGRPMMVGRPGVVEASLPSVDAGKRGLVVVGYMGTRD
                     RGDDDYSKVAWDGYVTSTRNPLARRPLFYSTSVNEPGDPLIRGECPQTKCGPEYDFID
                     VVVAPDDTVWAAFVDGCVALCSETFGPNDAYAGIASHIRVPR"
     gene            1304..1825
                     /locus_tag="VEV43_01775"
     CDS             1304..1825
                     /locus_tag="VEV43_01775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22276.1"
                     /translation="MIRPASAEDAAAILGIYAPAVRDSVATFELEPPSLDEMTRRIAE
                     VTRRYPWLVHEAGGRVAGYAYATEYHERPAYAWTCEVSVYVGDGARGTGAGRALLREL
                     LARLEAAGFVNAIARIALPNDASVKLFEGHGFEPIGTARGIGYKLGRWVDVGEWQRDL
                     AKRGPAPRPPSSP"
     gene            complement(1803..3032)
                     /locus_tag="VEV43_01780"
     CDS             complement(1803..3032)
                     /locus_tag="VEV43_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22277.1"
                     /translation="MLRSGRLLVTVAAATVVLAVSVHAAPPPELLQPGFAVTPFAATQ
                     GTPTSLAFGGGESPFLYVTDGTSGNVLVFDTAGLVLTFAEGFRSPLGIVVGPDGSVYV
                     SDVEAAREGPYGLRSYGRVWRLRDTDGDHVADKRNVVVKDLPNGRHNTNGLAFGPDGF
                     LYVANGNSTDDGVEGGESEVEPWSGSVVRVNPRARNVSVTKLPARVLVAHGWRNVYDL
                     AFSPVDPTKVFVPMNGADDARQGSTAENPADPNLEDSDDLLYVTDVDDRKVEDFAFPQ
                     CLYNLAERGDLKPYANPNPDVIDRFGRCRKRVVPRPVASFGLHPSANGLAFQTTDAWG
                     DDYRNDLFVAEWGSLFGAPTGHKVVRVELDDSGTKVTALSDFLEMDTPLDLTFHPDGS
                     MYVADYSGTIFKVTKAV"
     gene            complement(3032..3439)
                     /locus_tag="VEV43_01785"
     CDS             complement(3032..3439)
                     /locus_tag="VEV43_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP22278.1"
                     /translation="MRATRYAVAVASAALVLAASAPAHAAVAAAVPGSFAAGFATPVV
                     VAAQGEGITFVNADPAPHNFVADGVYLSKKEAKKARWCTSFSAKSCPLFWSPTVAAGE
                     TTEVEGLERLETGQQYPFFCSLHPGMKGTLVVR"
     gene            3520..4017
                     /locus_tag="VEV43_01790"
     CDS             3520..4017
                     /locus_tag="VEV43_01790"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYP22279.1"
                     /translation="MNDDTKETFEELAGHLDYPMYVVTAAADGVRAGCLVGFASQCSI
                     DPPRFMVFVSDKNHTHRVALESPALGVHVVPDEAEDLARLFGEQTGDEIDKFERCEWE
                     PGPHDVPLLARCGDRFVGSVLEKRSVGDHTAFLLEPLDVHAGGSSGSFFPFRKAQELD
                     PGHEA"
     gene            complement(4025..4726)
                     /locus_tag="VEV43_01795"
     CDS             complement(4025..4726)
                     /locus_tag="VEV43_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HYP22280.1"
                     /translation="MKRALVMGFVAALLVPNTVDAGLRRVLLGYSHEGRPIKAYRLGD
                     PASENVVLVVGCIHGNECAGKKVITALKKRPPPRGVKIWMIEALNPDGAAAGTRQNGR
                     GVDLNRNFPRKWRHQGARWSTYYSGPRAASEKETRHAMRFVDEIRPDVTIWYHQAMSL
                     VDRSGGRVAIQRRYARMVGLPLRRLDPLPGTATRWQNHHFDNSTSFVVELPGGELSWR
                     SAKTHARAVVTVGKM"
     gene            complement(4723..5370)
                     /locus_tag="VEV43_01800"
     CDS             complement(4723..5370)
                     /locus_tag="VEV43_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HYP22281.1"
                     /translation="MRRLLPLLLVAALLAPPSAVLAARRPETVEDHIPYGAKRKRQMA
                     RYSGRHYGLRRWKLRNPSVIVLHFTATSTYPPVRNHFASNSPARGEMPGVCTHYVVDK
                     DGTIYELVPLGIRCRHTIGLNHRAIGIEMVQETGRGSHWADRQILQRRPQIRAALRLV
                     RWLRDREGIALRDVIGHAMGNGHRRFEDRQGWRNDHTDWLWRDVKVFRKRLRRIS"
     gene            complement(5381..6172)
                     /locus_tag="VEV43_01805"
     CDS             complement(5381..6172)
                     /locus_tag="VEV43_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYP22282.1"
                     /translation="MWRDWLRAYADAWENADPGVVELFTEDATYLSQPFREPHTGRDG
                     IRAYWAGATATQRDTEVLIGDPLVAGDHVVAEWWTTMVEDDGPVTLPGVLLLDFDGDE
                     CRALREYWAYEPGRHEPFPEWGRFEGDQPAADHAAAWADAYERAWRAGDADAAAALYA
                     EDVSFRSHAFREPVRGRAAVRDYTARAFAAEEDRVARLGRPLAAGAGAAVEYWTTYVE
                     EGAAQTLAGCVLMSFDANGAVTTSRDYWHVADERRDPPAGWGRFG"
     gene            6281..8467
                     /locus_tag="VEV43_01810"
     CDS             6281..8467
                     /locus_tag="VEV43_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYP22283.1"
                     /translation="MLERLARWSYKRRWPMLGLWIAALIGFNVLASTAGGDYATDFSL
                     PGAESQEAYDLLRERFPQESGDNAQVVFRADGGVDDPEVQASVEALLADLAEIEEVAA
                     IQSPYDAEGARNVSESGEIAFANVLFDVKATDVPIELVDEMKAMARAAEGPGLDVALG
                     GPIIAFNEFEQPEGSEMVGLLAAVLILLVTFGSVLAMGLPILTALFGIGIGLALVLLF
                     AHVFSVPDFTTQLASMIGIGVGIDYALFIVTRYRQHLHNGMEPEEAVVKAITTSGKAV
                     LFAGTTVVISLLGILLMGFAFVEGVAIGGAATVLVTMMASVTLLPAILGFTGRNIDKL
                     RIPTFHRTESRNQVSMWFRWSRVVQRRPIPAAAAGFLVLLLLSIPLFSIRLGQADAGT
                     SAETKDSRQAYDLLAEGFGPGVTGPLILAARLGGPEGMADVERLADAVSASDRVAAVT
                     PPVPNPEGDTAVVTVFPTTSPQDPETNHLVHELRDEIVPSALEGTETEVFVGGATAST
                     IDFSEKNAERLPILIGTVLALSFLLLMVVFRSILVPLKAVVMNLLSIGAAYGVIVAIF
                     QWGWLKDVFGVASTAPIEAWVPMMLFTILFGLSMDYEIFLLSRIREEYLAKRDNALAV
                     ANGLATTARVITAAAAIMISVFLAFVFMIDERSVKLFAVGLAAAILVDATIVRMVLVP
                     ATMELLGDANWWFPRWLDRLVPRVSVEGEGEHLEMEGMREPALEKA"
     gene            complement(8464..8748)
                     /locus_tag="VEV43_01815"
     CDS             complement(8464..8748)
                     /locus_tag="VEV43_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP22284.1"
                     /translation="MNAAEIIKVARLNADLTQAELAKRLGTTQPQVVRWERGKTAPSF
                     QRVVDAVRACGFELGVRIHMASKEDAAAIEENLDLTPTEGLRRLSERNRC"
     gene            8823..10313
                     /locus_tag="VEV43_01820"
     CDS             8823..10313
                     /locus_tag="VEV43_01820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HYP22285.1"
                     /translation="MRRTLSFALLAALVAALVPSLAGAQVPPTPPRDQWFAENDTGHW
                     SDGDRVRIDESCLKLPKSKQLSCSLAAFERKPFVVIAFVDSGVNPYHQDFRAPEFVHH
                     PSKFVEGYPAEAQALKPSLALADSKGYDAARAKDDAMWKTVKRDQLYWFPGTRIIGGI
                     SRAAGDVSGEWQERGILDDQGHGTGVVSVGAGQHFGSNPNALIVMVEGLGERSLEWAT
                     AQPWIDIVSNSWGNTANLPLGDPESGRTATRRGQSIVWSAGNGATNTNSSTVFPAGGP
                     VEDPCKCKTPDSDNSITDPWSGPAWLLTVGAASPINGQAHWWHSIPVDVASFGSKWRA
                     AGAWGVRPVIDEANVETRDFGGTSCATPVTAGVLSAIIERARIELGDTTGGQRPDQVV
                     AIGKKKLASGPLDDGKLTRLEAEAIVQKTAFPVEADPEKITWDYAVRPTTPTYYLHQG
                     YGVVTRDSKALALDVLLGKEELPVRTEVDTFISAMDQIRNAKWGEP"
     gene            complement(10310..11371)
                     /locus_tag="VEV43_01825"
     CDS             complement(10310..11371)
                     /locus_tag="VEV43_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HYP22286.1"
                     /translation="MRTGRADLPLHTGRAPAWLFDRMRSLAREIGRAIVEEEGPHGLL
                     RRAADPFWFQAFGCVLGFDWHSSGLTTTACGALKEGLRPLFSELGIVVAGGKGKTSLK
                     TPAEIETACDSIGLDAAPLVRASRLSAKVDSSAVQDGYDVYHHTFLFSSDGAWTVVQQ
                     GMNQSNGMARRYHWHTAPTFVSDPHAGVAGRAERDVTNLVAAAGGRNRDVSVELAREA
                     PSKVAAEIVRMRSLEMPRHHFVRFADLHPDRVERVLLKAYEAQPEDYTSLLSVPGVGA
                     KGLRALALVAEVTYGAPASVKDPVSFSFAHGGKDGTPFPVDRATYDATIESLKKAVDE
                     TRAGRSEKRDALRRLARLA"
     gene            11434..12108
                     /locus_tag="VEV43_01830"
     CDS             11434..12108
                     /locus_tag="VEV43_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22287.1"
                     /translation="MFYGGFVYGILAPLTILGVIVLAILALSGRSDPDTRGDRARVLY
                     LSLVSFIALFTVLFALVSLGSTAARSLLDDGQSECIDPSDPACFGIDAEFGGGEQHTR
                     ELLNSTGVAVVAAAVLVFHRRRTREVVDDPGFAGSAAARTFNAYLYAVAFTAMVLLLA
                     AAAVALPALIRTIAPGLTATGDSSFERDAALLDLVPALVAGAGAAVIYLTHWRAASAL
                     RRGGND"
     gene            12176..>13917
                     /locus_tag="VEV43_01835"
     CDS             12176..>13917
                     /locus_tag="VEV43_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP22288.1"
                     /translation="MTTTRTHLSAAAALALVLAATVVAPETASAAPSSCFGRRPTVVG
                     TNGPDDLSGTPGVDVIVGRGGNDTIFGLAGNDFLCGNRGNDTIQAAEGADRASGGTAR
                     DFIRGGSGSDFLYGNAGNDHLDANDGADFVSGGKGSNSLEGGDGPDRLVGGRNSDSLA
                     GGDGDDVLEAGACEKEKRQRTCTNYLSAGEGEDRLIGGPDNDYFAPGMGDDEIDGKGG
                     KDGIAFDYSDRKQQRGARVDLAEGRAHSVEGSDRLTGVEIVYGTFYDDRITGNGDANK
                     LYGRNGNDSIRALGGNDVVAGGAGNDVLGGGDDKDRVEFIESYGVVVNLEIGTASGEG
                     DDLVSDFEDIGGTGGLDTLIGNAGPNEIRGGGGEDTLRGGAGNDTLVGGDEYADPALG
                     YFFGDSLDGGAGDDVMLGGLGYDAVDFGTAPGPVTASIATGVATGHGTDSFLEVEDLL
                     GSGYADTLTGDDALNHLTGRSGDDAMDGAGGDDVLVGSNGNDAMEGGAGVDLADYASA
                     AAAVTANLTTTQATGQGNDTLTGIEDLSGSIFDDSFTGDEGPNHILGLSGDDTIEGAG
                     GDDLLEANYGDDTTR"
BASE COUNT         2069 a         4864 c         4883 g         2101 t
ORIGIN      
        1 ccggaggggc cgtggccgcg acggccgccg gcggtctcga cggacgcgcg gtcgcgcatc
       61 gggccggggg ccgggccgtc caagtcgagc gactcccgcg ggtcgagacg ttctcgatcg
      121 gccacaacgc cgccgagccg accctcggcg tcacggccga aggcgacgtc ttctatgcgg
      181 cggcggaccc gacgacgctc gttcctccgc ttcccgtcga catcatgcgt tcgaccgacg
      241 ggggccagac gtgggaggtg aagtcgccgc agctcgcggg gaacaacgtg cacgcttcca
      301 ccggcgaccc gtacgtgtac gtcgaccgca cgcgggccgg ggcacgcgtc ttcaccgtcg
      361 atctccaggg ctacgcgtgc tcggtgctct cgttcagtga cgacggaggg gacagctggg
      421 tcacgaaccc gctcgggtgc gggcggcccg tcaacgacca ccagacggtg ttcgccgggc
      481 cgccggtcga gagctcgacg ctcggctacc cgcacgtcgt ctactactgc taccacgaca
      541 cggtcacgtc ctcttgtacg aagtcgctcg acggcgggat cacgttcgtc ccgacgggcg
      601 agccctcgta caccgcgtac acggagtccg gggagttctg cggcgggttg cacggacacg
      661 gcttcgccgg tcccgacggg accgtctaca tcccgaaggg gcattgcggc gtcccggtcc
      721 tggcgatcag caaggacgag ggcgcgtcgt ggacgcgcgt cgaggtggcg aagctcccgt
      781 cggccgggca cgaggcgagc gtcgcggccg acaagcgcgg gaacgtcttc tacgcgtaca
      841 tagggatcga catgctgccg tacctggtcg tgtcccgcga cggcggttcg acgtggggtc
      901 gtccgatgat ggtcggccgt ccgggcgtcg tcgaggcgag cctgccgtcc gtcgacgcgg
      961 gcaagcgcgg cctggtagtc gtcggctaca tggggacgcg tgaccgcggc gacgacgact
     1021 actcgaaggt cgcgtgggac ggttacgtga cctcgacgcg gaatccgctg gcccggcgcc
     1081 cgctgttcta cagcacgtcc gtgaacgagc cgggcgaccc tctgatccgc ggcgagtgcc
     1141 cccagacgaa gtgcggtccc gagtacgact tcatcgacgt ggtcgtggcg ccggacgaca
     1201 cggtgtgggc agcgttcgtc gacggttgcg tggcgctgtg ctcggagacg ttcgggccga
     1261 acgacgccta cgcgggcatc gcttcgcaca tccgcgtccc gcgttgatcc gcccggcgag
     1321 cgccgaggac gcagcggcga tcctcgggat ctacgcgccg gccgtgcgcg actcggtcgc
     1381 gacgttcgag ctcgagccgc cgtcgctcga cgagatgacc cggcggatcg ccgaggtgac
     1441 gcgacgctat ccgtggctcg tgcacgaggc cgggggccgg gtcgccggct acgcgtatgc
     1501 gaccgagtac cacgagcgcc cggcctacgc atggacgtgc gaggtgtcgg tgtacgtggg
     1561 cgacggtgcg cgggggacgg gggccgggag ggcattgctc cgggagctgc ttgcgcgcct
     1621 ggaggcggcg ggcttcgtga acgcgatagc gcgcatagcg ctgccgaacg acgcgagcgt
     1681 gaagctgttc gagggccacg gcttcgagcc gataggcacg gcccgaggga tcggctacaa
     1741 gctgggccgc tgggtggacg taggggagtg gcagcgagac ctagcgaagc gggggccggc
     1801 ccctagaccg ccttcgtcac cttgaagatc gtccccgagt agtcggccac gtacatcgac
     1861 ccgtcggggt ggaacgtcag gtccagcggg gtgtccatct ccaagaagtc cgacagcgcc
     1921 gtcaccttcg tcccggagtc gtccagctcg acccgcacta ccttgtgccc cgtcggcgcc
     1981 ccgaagaggc tgccccactc ggccacgaac aggtcgttgc ggtagtcgtc gccccacgcg
     2041 tcggtcgtct ggaacgcgag gccgttcgct gaggggtgga ggccgaacga cgcgacgggg
     2101 cgcgggacga ctcgcttgcg acaacgcccg aagcgatcga tcacgtccgg gttcgggttc
     2161 gcatacggct tcaggtcccc gcgctcggcg aggttgtaca ggcactgcgg gaacgcgaag
     2221 tcctcgacct tccggtcgtc gacgtcggtc acgtacagca ggtcgtcgga gtcctcgagg
     2281 ttggggtcgg ccgggttctc ggcagtcgag ccctggcgcg cgtcgtcggc tccgttcata
     2341 ggcacgaaca ccttcgtcgg gtcgaccggc gagaacgcga ggtcgtagac gttgcgccat
     2401 ccgtgcgcga cgagcacccg ggccggcagc ttcgtgaccg agacgttcct ggcccgcggg
     2461 ttgacccgga cgaccgagcc ggaccaaggc tcgacctcgg actcgccccc ctcgacgccg
     2521 tcgtcggtcg agttcccgtt cgcgacgtag aggaacccgt cgggcccgaa cgcgaggccg
     2581 ttggtgttgt ggcgtccgtt cgggaggtcc ttcaccacga cgttgcgctt gtcggcgacg
     2641 tggtcgccgt cggtgtcgcg cagccgccac acgcgcccat agctccggag cccgtacggc
     2701 ccctcgcgcg cggcctcgac gtcggacacg tacacgctcc cgtcggggcc gacgacgatg
     2761 ccgagcgggg atcgaaaccc ctcggcgaac gtaaggacga ggccggccgt gtcgaacacg
     2821 aggacgttcc cgctcgtgcc gtcggtcacg tacaggaagg gcgactcacc gccgccgaac
     2881 gcgaggctcg tgggggtccc ctgcgtcgcg gcgaagggcg tgacggcgaa gccgggctgg
     2941 aggagctcag gcggcggcgc cgcgtggacg gagaccgcca ggaccaccgt ggccgcggcg
     3001 accgttacga ggagacgccc gctacggagc atcagcggac gaccagcgtc cccttcatgc
     3061 ccgggtggag gctgcagaag aacgggtact gctgaccggt ttcgaggcgt tcgagcccct
     3121 cgacctcggt ggtctcgcct gcggccaccg tcgggctcca gaacagcggg cagctcttgg
     3181 ccgagaacga ggtgcaccag cgcgccttct tcgcttcctt cttcgacagg tagacgccgt
     3241 cggcgacgaa gttgtgcggg gccgggtcgg cgttgacgaa cgtgatgccc tcaccctggg
     3301 cggcgaccac gacgggcgtg gcgaagccgg cggcgaagct cccggggacc gcggcggcga
     3361 ccgctgcgtg ggcgggggcc gaggcggcga ggacgagtgc cgccgaggcg accgcgacgg
     3421 cgtaacgagt ggctctcatg ctcgaaaagg ttcaccaccg cggccccttt ctccttcacc
     3481 cggtcgggac cgcgtgcggc cggatgccat ccttggcgga tgaacgacga cacgaaagag
     3541 acgttcgagg agctcgccgg gcacctcgac tacccgatgt acgtcgtgac cgccgccgca
     3601 gacggagtgc gggccgggtg tctcgtcggc ttcgcctcgc aatgcagcat cgacccgcct
     3661 cgcttcatgg tcttcgtgtc cgacaagaac cacacgcacc gggtcgcgct cgagtcaccg
     3721 gcgctggggg tccacgtcgt cccggacgaa gcggaggacc tggcgcggct cttcggcgag
     3781 cagaccggtg acgagatcga caagttcgag cggtgcgaat gggagccggg cccccacgac
     3841 gtcccgttgc tggcgcgctg cggcgatcgt ttcgtcggga gcgtgctcga gaagcgatcg
     3901 gtcggcgacc acacggcttt cctgctcgag cccctcgacg tccacgccgg cggttcgtcg
     3961 ggctccttct tcccgttccg caaggcacag gagctcgatc cgggccacga ggcctgacgc
     4021 gtccctacat cttccccacg gtcacgaccg cgcgggcgtg cgtcttcgcg gaacgccacg
     4081 acagctcgcc gcccgggagc tcgacgacga acgacgtcga gttgtcgaag tgatggttct
     4141 gccagcgcgt cgcggttccc ggcaggggat cgagccggcg cagcgggagc ccgaccatcc
     4201 gcgcgtagcg gcgctggatc gcgacgcggc cccccgaccg gtcgacgagc gacatcgcct
     4261 ggtggtacca gatcgtgacg tcgggacgga tctcgtcgac gaacctcatc gcgtggcgcg
     4321 tctccttctc cgacgcggcc cgcggccccg agtaataggt gctccaacgc gcgccctgat
     4381 gccgccactt gcgcgggaag ttccggttga ggtcgacgcc gcgtccgttc tgccgcgtgc
     4441 cggcggccgc gccgtcgggg ttgagggcct cgatcatcca gatcttcacg ccgcgcggcg
     4501 gcggcctctt cttgagcgcg gtgatcacct tcttccccgc gcactcgttg ccgtggatgc
     4561 agccgacgac gagcacgacg ttctcgctcg ccgggtcgcc gagccggtac gccttgatcg
     4621 ggcggccctc gtgcgagtag ccgagcagca cgcgcctcag ccccgcgtcg accgtgttgg
     4681 ggaccaggag cgcggcgacg aagcccatga ccagcgcgcg cttcacgaga tcctccgcag
     4741 ccgcttgcgg aagaccttga cgtcgcgcca cagccagtcg gtgtggtcgt tgcgccagcc
     4801 ctgccggtcc tcgaaccgac ggtggccgtt gcccatcgcg tgcccgatga cgtcgcgcag
     4861 cgcgatccct tcccggtctc gtagccaccg caccagtcgc agcgccgcgc ggatctgcgg
     4921 gcgtcgttgg aggatctgcc ggtccgccca atgggagccg cggcccgtct cctgcaccat
     4981 ctcgatcccg atcgcgcggt ggttgagccc gatcgtgtgc cggcaccgga tccccagagg
     5041 gacgagctcg tagatcgtcc cgtccttgtc gacgacgtag tgcgtgcaca cgccgggcat
     5101 ctcgccgcgt gccggtgagt tcgaggcgaa gtggttgcgg acgggcgggt aggtgctcgt
     5161 cgcggtgaag tgcaggacga tcacgctcgg gttgcgcagc ttccaccgcc tcagcccgta
     5221 gtgacggccg gagtagcggg ccatctggcg cttgcgcttc gctccgtacg ggatgtggtc
     5281 ctcgacggtc tccggtcgcc gcgctgcgag caccgccgaa ggcggcgcga ggagggccgc
     5341 gaccaggagc aggggcagga gccggcgcat cgcgcccacg ctagccgaac cggccccaac
     5401 ccgccggagg gtcgcgtctt tcgtccgcga catgccagta gtcccgagac gtcgtgaccg
     5461 cgccgttggc gtcgaacgac atcagcacgc atccggccag cgtctgcgcg gccccctcct
     5521 cgacgtacgt cgtccagtac tcgaccgccg cgccggcgcc cgccgctagc ggccggccga
     5581 gtcgtgcgac tcgatcttcc tcggcagcga aggccctcgc ggtgtagtcg cgcaccgcgg
     5641 cccggccccg taccggctct cggaacgcgt gcgagcggaa cgagacgtcc tccgcataga
     5701 gcgcggccgc cgcatcggcg tccccggcgc gccacgccct ctcgtatgca tcggcccagg
     5761 cggccgcgtg gtccgccgcg ggctggtcgc cctcgaacct gccccactcg gggaacggtt
     5821 cgtggcgacc cggctcgtac gcccagtact cgcgcagcgc gcggcactcg tccccgtcga
     5881 agtcgagcag caggacgccc ggcagcgtga ccgggccgtc gtcctcgacc atcgtcgtcc
     5941 accactccgc cacgacgtga tcgcctgcga cgagggggtc gccgatcagg acctcggtgt
     6001 ccctctgggt cgcggtcgcg ccggcccagt aggcccggat cccgtcacga ccggtgtgcg
     6061 gctcgcggaa cggctgtgac aggtacgtcg cgtcttcggt gaacagctcc acgacgccgg
     6121 ggtcggcgtt ctcccacgcg tccgcgtacg cgcgcagcca atctctccac atccgtcgag
     6181 agtacggaca aagcggacta cgcagttgcc gcaaagcgga gccgcgtcgc tgcgccgctc
     6241 gttagcgtgg ggtcaactct ctagaaaggc aggagcgacg atgctcgaac gtctcgcgcg
     6301 ctggagctac aagcgccgct ggcccatgct cggtctctgg atcgcggccc tgatcggctt
     6361 caacgtcctg gcgtcgacgg ccggcggtga ctacgcgacg gacttctcgc ttccgggggc
     6421 cgagtcgcag gaggcgtacg acctcctgcg cgagcgtttc ccgcaggagt ccggcgacaa
     6481 cgcgcaggtc gtcttccggg cagacggcgg tgtcgacgac cccgaggtgc aggcttcggt
     6541 cgaggcgctg ctcgccgatc tcgcagagat cgaagaggtc gccgccatcc agagcccgta
     6601 cgacgccgag ggagcgcgca acgtctccga gagcggcgag atcgcgttcg cgaacgtgct
     6661 gttcgacgtc aaggcgaccg acgtcccgat cgagctcgtc gacgagatga aggcgatggc
     6721 ccgcgccgcg gaagggcccg gcctcgacgt cgcgctgggc gggccgatca tcgcgttcaa
     6781 cgagttcgag cagcccgaag ggtcggagat ggtcgggctc ctcgcggcgg tcctgatcct
     6841 gctcgtgacg ttcggctcgg tcctcgcgat gggcctcccg atcctcaccg cactgttcgg
     6901 gatcggcatc ggcctcgccc tcgtgctcct gttcgcgcac gtgttcagcg tcccggactt
     6961 cacgacgcag ctcgcgtcga tgatcggcat cggcgtgggc atcgactacg cgctgttcat
     7021 cgtcacccgc taccggcagc acctccacaa cggcatggag cccgaggaag cggtcgtcaa
     7081 ggcgatcacg acgtcgggca aggcggtgct gttcgccggc acgaccgtcg tgatctcgct
     7141 gctcgggatc ctgctcatgg gcttcgcctt cgtcgagggc gtcgcgatcg ggggcgcggc
     7201 gacggtgctc gtgacgatga tggcgtcggt gacgttgctg ccggcgatcc tcggcttcac
     7261 cgggcgcaac atcgacaagc tcaggatccc gacgttccac cggaccgaga gccggaacca
     7321 ggtctcgatg tggttccggt ggagccgcgt cgtccaacgg aggccgatcc ccgccgcggc
     7381 cgccgggttc ctcgtcctgt tgctgctctc cataccgctg ttctcgatcc gcctcggcca
     7441 ggccgacgcg gggacctcgg cggagacgaa ggactcccgt caggcgtacg acctgctggc
     7501 cgagggattc ggccccgggg tcaccggccc gctgatcctc gccgcgcggc tcgggggccc
     7561 cgaggggatg gccgacgtcg agcggctcgc cgacgcggtc tccgcgagcg accgcgtcgc
     7621 ggcggtgacg ccacccgtcc ccaacccgga aggggacacc gcggtcgtca cggtcttccc
     7681 gacgacgtca ccgcaggacc ccgagacgaa ccacctcgtg cacgagctgc gcgacgagat
     7741 cgtgccgtcc gcgctcgagg ggaccgagac cgaggtcttc gtcggcggcg cgaccgcgag
     7801 cacgatcgac ttctcggaga agaacgccga acggctgccg atcctcatcg ggaccgtgct
     7861 cgcgctctcg ttcctgttgc tgatggtcgt cttccgttcg atcctcgtcc cgctcaaggc
     7921 cgtggtaatg aaccttctgt cgatcggggc cgcgtacggg gtcatcgtcg cgatcttcca
     7981 gtggggctgg ctgaaggacg tgttcggggt cgcgtcgacc gcgccgatcg aggcgtgggt
     8041 gccgatgatg ctgttcacga tcctgttcgg gctgtcgatg gactacgaga tcttcctgct
     8101 gtcgcggatc cgggaggagt acctggcgaa gcgcgacaac gcgctcgcgg tggcgaacgg
     8161 cctggcgacg accgcgcggg tgatcaccgc ggcggccgcg atcatgatct cggtcttcct
     8221 cgcgttcgtg ttcatgatcg acgagcggtc ggtgaagctg ttcgccgtcg ggctcgcggc
     8281 ggcgatcctc gtggacgcga cgatcgtgcg gatggtgctg gtcccggcga cgatggagct
     8341 gctgggcgac gcgaactggt ggttcccgcg ctggctggac cgcctggtgc cccgggtctc
     8401 ggtcgaaggg gagggggagc acctggagat ggagggcatg cgggagccgg ccctggagaa
     8461 ggcctagcag cggttccgct ccgaaagacg gcggagcccc tcggtcggcg tcagatcgag
     8521 gttctcctcg atcgccgcgg cgtcttcttt gctcgccatg tggattctca cgccgagctc
     8581 gaatccgcac gcgcgcacgg cgtccacgac ccgctggaag gacggcgctg tcttgcccct
     8641 ctcccatcgc acgacctgcg gctgggtcgt cccgagccgc ttcgccagct cggcttgcgt
     8701 caggtcagcg ttcagccgag ccaccttgat gatctcggcc gcgttcacgg gactcattat
     8761 atcaatactg atatagatcg ggaggcagag gagatcggcc cccgggggct aatccctctg
     8821 ccatgcgcag aacactctct ttcgccctgc tcgccgccct cgtggccgcg ctcgtcccgt
     8881 ccctcgccgg tgcccaggtg ccgccgaccc cgccccgcga ccagtggttc gccgagaacg
     8941 acaccggcca ttggtcagac ggcgaccgcg tccggatcga cgagagctgc ctgaagctcc
     9001 cgaagtccaa gcagctgtcg tgctcgctcg cggccttcga gcgcaagccg ttcgtcgtga
     9061 tcgcgttcgt cgactcgggc gtcaacccgt accaccagga cttccgggcc cccgagttcg
     9121 tccaccaccc gtcgaagttc gtcgagggct atccggccga ggcgcaggcg ctgaagccgt
     9181 cgctcgcgct cgcggacagc aagggctacg acgcggctcg cgcgaaggac gacgcgatgt
     9241 ggaagaccgt gaagcgcgac cagctctact ggttccccgg cacccggatc atcggcggca
     9301 tctcgcgcgc cgccggcgac gtctccggcg agtggcagga gcgcggcatc ctcgacgacc
     9361 agggccacgg aaccggtgtg gtctccgtcg gcgccggtca gcacttcggc tcgaacccga
     9421 atgctttgat cgtgatggtc gagggcctcg gcgagcgcag cctcgagtgg gcgaccgcgc
     9481 agccgtggat cgacatcgtc tcgaactcgt ggggcaacac ggcgaacctc ccgctcggcg
     9541 accccgagtc cggccgcacc gccacccgcc gcggccagag catcgtgtgg tcggcaggca
     9601 acggtgcgac gaacacgaac tcgtcgacgg tcttcccggc cggcgggccc gtcgaggacc
     9661 cgtgcaagtg caagacgccg gactccgaca actcgatcac cgacccgtgg tccggacccg
     9721 cgtggttgct gaccgtcggc gcggcgtcgc cgatcaacgg ccaggcgcac tggtggcact
     9781 cgatccccgt cgacgtcgcg tcgttcggat cgaagtggcg cgctgcgggg gcctggggcg
     9841 tcaggccggt catcgacgaa gccaacgtcg agacgcgtga cttcggcggc acctcttgcg
     9901 cgacccccgt gaccgccggc gtcctctccg cgatcatcga gcgggcgcgc atcgagctcg
     9961 gcgacaccac cggcgggcag cgtcccgacc aggtcgtcgc gatcggcaag aagaagctcg
    10021 cgtcgggacc gctggacgac gggaagctca cccggctgga ggccgaggcg atcgtgcaga
    10081 agaccgcctt cccggtcgaa gccgaccccg agaagatcac gtgggactac gcggtgcggc
    10141 cgacgacccc gacgtactac ctgcaccagg gctacggcgt cgtgacgagg gactcgaagg
    10201 cgctcgccct cgacgtgctg ctcggcaagg aggagctccc ggtccgcacc gaggtcgaca
    10261 ccttcatctc cgcgatggac cagatccgca acgcgaagtg gggcgagccc taagccagcc
    10321 gggccagcct ccgcagcgcg tcccgcttct ccgaacggcc cgctcgggtc tcgtcgaccg
    10381 ccttcttcag cgactcgatc gtcgcgtcgt aggtcgcgcg gtcgaccggg aacggcgtgc
    10441 cgtccttgcc cccgtgcgcg aacgagaacg acaccgggtc cttcaccgac gccggcgcgc
    10501 cgtacgtcac ctcggccacc agtgccagcg cgcgtaagcc tttcgcgccg acgcccggca
    10561 ccgagagcag cgacgtgtaa tcctcgggct gcgcctcgta cgccttcagc aacacccgct
    10621 cgaccctgtc cgggtgcagg tccgcgaagc gcacgaagtg gtggcgcggc atctccagcg
    10681 accgcatccg cacgatctcg gcggcgacct tcgagggggc ttcgcgcgcc agctcgaccg
    10741 acacgtcgcg gttgcgcccc cccgcggccg cgacgaggtt cgtcacgtcg cgctccgcac
    10801 ggcccgcgac tcccgcgtgg gggtccgaca cgaacgtcgg cgcggtgtgc cagtggtagc
    10861 ggcgcgccat cccgttcgac tggttcatgc cctgctgtac gaccgtccac gcgccgtccg
    10921 acgagaacag gaacgtgtgg tggtagacgt cgtagccgtc ctgcacggcc gacgagtcga
    10981 ccttcgccga caaccggctc gcacgcacga gcggcgcggc gtccagccct atcgagtcgc
    11041 acgcggtctc gatctccgcc ggcgtcttca gcgacgtctt ccccttgcct ccggcgacga
    11101 cgatcccgag ctcggagaac agcgggcgga gcccttcctt cagcgcgccg cacgcggtcg
    11161 tcgtgaggcc gctcgagtgc cagtcgaagc ccagcacgca cccgaacgcc tggaaccaga
    11221 acgggtcggc ggcgcgtcgc agcagcccgt gggggccttc ctcttccacg atcgcgcgtc
    11281 cgatctcgcg cgcgagcgac ctcatccggt cgaagagcca tgcgggagcg cgcccggtgt
    11341 ggagcgggag gtcggctcgc ccggtgcgca tcctgcgagc ttaggagcat cgtccgtata
    11401 gtcctggtac ggcgactagg caggggaggc ggcatgttct acggcgggtt cgtctacggc
    11461 atcctcgctc cactcacgat cctgggcgtg atcgtgctcg cgatactcgc gttgtcgggg
    11521 aggtcggacc cggacacccg aggcgacagg gcccgcgtgc tctatctgag cctcgtctct
    11581 ttcatcgcgc tgttcacggt cctgttcgct ctcgtcagcc tcggatcgac cgcggcacgt
    11641 tccttgctcg acgatggcca gtcggagtgc atcgacccgt ccgaccctgc ctgcttcggt
    11701 atcgacgccg aattcggtgg cggtgagcaa cacacgcgcg aattgctcaa ctcgacgggc
    11761 gtggcggtcg tcgccgctgc cgtgctcgtg ttccatcgcc gccggacacg agaggtcgtc
    11821 gacgatcccg gcttcgcggg ctcggcggcc gcgcgtactt tcaacgccta cctctacgcg
    11881 gttgcgttca cggcgatggt gctcctgctg gccgcggctg ccgtggcgtt acccgcgctg
    11941 atcaggacga tcgcgccggg cctcacggcg accggcgaca gctcgttcga acgcgacgcc
    12001 gcgctgctcg acctcgtccc ggccctcgtc gcgggggccg gagccgccgt catctacctg
    12061 acgcactggc gggcggcgtc cgccctgcgg cgcggcggga acgactaggg gacccattcg
    12121 ccctcccggg gcgtctatga cacggatccg acccaacggt gccaggagac gacaaatgac
    12181 gactactcga acccacctct cggcggccgc cgcgctcgcg ctcgtgctgg cggcgaccgt
    12241 cgtcgccccg gagacggctt ccgccgcgcc ctcgtcctgc ttcggcagac gcccgacggt
    12301 ggtaggcacg aacggtcccg acgacctcag cggcacgccg ggggtcgacg tcatcgtcgg
    12361 ccggggcggc aacgacacga tcttcggcct cgccggcaac gacttcctct gtggcaaccg
    12421 gggcaacgac acgatccagg ccgcggaggg cgccgaccgc gcctccggag ggaccgcgcg
    12481 cgacttcatc cggggcggca gcggcagcga cttcctctac ggcaacgcgg gcaacgacca
    12541 cctcgacgcg aacgacggag cggacttcgt gtcgggcggc aaggggagca actccctcga
    12601 gggaggtgac ggtcccgacc ggctcgtcgg cggccgcaac tccgactcgc tggccggagg
    12661 cgacggggac gacgtgctcg aagccggcgc gtgcgagaag gagaagcgcc agcgcacgtg
    12721 cacgaactac ctcagcgccg gtgaagggga ggaccggctg atcggtgggc ccgacaacga
    12781 ctacttcgcg cccggcatgg gcgacgacga gatcgacggg aagggcggca aggacgggat
    12841 cgcgttcgac tactcggatc gcaagcagca gcgcggcgca cgggtcgacc tcgctgaggg
    12901 gcgggcgcac agcgtcgagg gctcggaccg cctgacgggc gtcgagatcg tctacggcac
    12961 cttctacgac gacaggatca ccggcaacgg cgacgcgaac aagctgtacg gaaggaacgg
    13021 caacgactcg atccgcgcgc tcggcggcaa cgacgtggtg gcgggcggag ccggcaacga
    13081 cgtgctcggc ggcggtgacg acaaggaccg cgtcgagttc atcgagtcgt acggcgtcgt
    13141 cgtcaacctc gagatcggga cggcgagcgg agagggcgac gatctcgtgt cggacttcga
    13201 ggacatcggc ggcaccgggg ggctcgacac gctcatcggc aacgcgggcc cgaacgagat
    13261 ccgcggtggc ggcggtgagg acacgctgcg cggcggggcc ggcaacgaca cgctcgtggg
    13321 cggcgacgag tacgcggacc cggccctcgg ctacttcttc ggcgactcgc tcgacggcgg
    13381 cgccggcgac gacgtgatgc tcgggggcct cggctacgac gcagtcgact tcgggaccgc
    13441 gcccgggccg gtcaccgctt cgatcgcgac cggggtcgcg accggccacg ggaccgacag
    13501 cttcctcgag gtcgaggacc tgctcggctc cggctacgcg gacaccttga ccggcgacga
    13561 cgcgttgaac catctgaccg gacgctcggg cgacgacgcg atggacggcg cgggtggcga
    13621 cgacgtcctc gtcggcagca acggcaacga cgcgatggag ggcggcgcgg gggtcgacct
    13681 cgccgactac gcgagcgcgg ctgctgccgt cacggcgaac ctcacgacca cgcaggcgac
    13741 cgggcagggc aacgacaccc tcaccgggat cgaggacctc tccggctcga tcttcgacga
    13801 ctccttcacg ggagacgagg ggccgaacca catcctcggc ctcagcggcg atgacaccat
    13861 cgagggagcc ggcggtgacg atctcctcga ggcgaactac ggagacgaca cgacgcg
//
LOCUS       DASQDO010000056         5623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_557533, whole genome shotgun sequence.
ACCESSION   DASQDO010000056 DASQDO010000000
VERSION     DASQDO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557533"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VEV43_01840"
     CDS             complement(<1..255)
                     /locus_tag="VEV43_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HYP22289.1"
                     /translation="MDEKTLNAFLAEYADQLPTLNALTPRLERLLHDLLCQYPFAIHS
                     IKARTKDRSRLARKLEEHDPSYESLADDHDLVGARIITYFP"
     gene            complement(284..1372)
                     /locus_tag="VEV43_01845"
     CDS             complement(284..1372)
                     /locus_tag="VEV43_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYP22290.1"
                     /translation="MQTSDARVLVLGEPEDAGRLEAALRSAGYEDLHVANDLTAPAEL
                     CRAVEPAVVLIDLTGPEYAGLDALRALCPSDGSSPSVAVVAAPSDAGARSASLALGAK
                     DFIGKPLDEDEVRIRVGNLIEAASLARRVSHQEHTAQQEIRRRTSELWDSLMRLEEAR
                     KHLQTAQEETLSRLSMAAEYRDDETSGHVERMSRYCALLARANGADSETSETLRLASR
                     LHDIGKIGVSDLILLKEGILTPTERRMMEEHVPIGVRLLEGSDSPVVQIAAIVAGSHH
                     EKYDGTGYPRGLAGDSIPIEGRIAAIADVFDALTHSRPHRRAFPLGHVLEMMRKESGK
                     HFDPALLDLFLDRMNTVLTIYEEHRESA"
     gene            complement(1541..2329)
                     /locus_tag="VEV43_01850"
     CDS             complement(1541..2329)
                     /locus_tag="VEV43_01850"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HYP22291.1"
                     /translation="MTGSLHITTAPGEEQRPARGPKAVEADVDLENFILITHAVPAAR
                     VKPQLPDKVSLETFEGADGRETCFVTTACFCNRDLRPRLTNRPTHTFNQVTFRTYVTY
                     GEARGLYFFGTYCDTIGSWLWQGAMATGTKMASFEHDMHKDGRGYHRYSCTARSGAEE
                     VAWLAEATDAPAPIHPFTTADEHVQFLGYRLHGFSRNPFGFYTHGRIDHRRLRPFSGR
                     LLEGRFDLWTRLGILDPAEYLSPASVLVEPHIRFTLMPPRPVRL"
     gene            complement(2337..3467)
                     /locus_tag="VEV43_01855"
     CDS             complement(2337..3467)
                     /locus_tag="VEV43_01855"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYP22292.1"
                     /translation="MSRLTHLECARCGRAHDADVLQNRCGCGGTLLARYDMSPLDLAE
                     VRARPPGMWRYRELLPVRGEPVSLGEGPTPLTSAPRLSERFGVEVFVKDDGPLPSGTF
                     KARGAAAALSRAVELGAESVVMPSAGNAGAAWSLYAARAGLTMTVTMARSAPEENKNE
                     VRLAGAELVEVEGTLADAAGRARAIADETGAFLAATFAEPYRVEGKKSAWLECFDQLG
                     RLPGTVVTSVGGGVAAVACSKAVEEVTALGWYEGSGPVIAGVQAEGCAPIVSAFEAGA
                     GAAEPWAAEPDTIAAGLRVPAPSEADLVLKVVRGSGGTMVAVSDDEIVTAVRDLAAAE
                     GILVCPEGASTLAGLAHLGELRGPVVLYNTGAAGKYLGALTR"
     gene            complement(3472..3786)
                     /locus_tag="VEV43_01860"
     CDS             complement(3472..3786)
                     /locus_tag="VEV43_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HYP22293.1"
                     /translation="MPTKEEIREEVVEALKTVNDPELGIDIWHLGLVYEVAVDDDHNV
                     KVEFTLTTMGCPIGPMLDEEIKAATAYIEGIGEVTTEMVMYPPWTPEKMDPLAKSALG
                     FV"
     gene            complement(3818..4432)
                     /locus_tag="VEV43_01865"
     CDS             complement(3818..4432)
                     /locus_tag="VEV43_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22294.1"
                     /translation="MKRLLVVALCASAGVLWHGDTSQNVADLPGVEDPREGPRVVLDV
                     ERARAARPAAITGNQVAFRLHTYEPFSNADLEHVEGKDYAVAIGISTDSDNAFERLCY
                     VDVRGDSVDQDGFTPFAAVTRGKRFPEAEPNPFSVREEFLGYALVRRPDPDSIEVTIP
                     PSLLALPPSRRFHWQARMVSPTEAGAAYHDYVPDARLARGRGGK"
     gene            complement(4536..5381)
                     /locus_tag="VEV43_01870"
     CDS             complement(4536..5381)
                     /locus_tag="VEV43_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYP22295.1"
                     /translation="MPRAIDFHVHVSRAEWLEGSIGPMLEVTAKYFRSEVRVRTAEEM
                     AAEYKEWDLLGVLLAWDAETATGLPPLTNDRVAEICREFPEQFVGFASVDPHKPDAVE
                     ELDRAVVELGMKGLKIHPQVQAFYPNDPQYDPLWDKCEEHALPIVVHVGQTGLGAGVP
                     GGSGIAFDYGRPMLMDMVAAHHPGLTVVMAHFGYPWHLEVLASAMHKTNVWIDLSGWR
                     PKYIPAEVKRDARGRLSDRFVWGSDYPMLDPGRILDEFDEMGLGDKEEDVLKNNAARL
                     LGLDV"
     gene            complement(5392..>5623)
                     /locus_tag="VEV43_01875"
     CDS             complement(5392..>5623)
                     /locus_tag="VEV43_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYP22296.1"
                     /translation="GSSTQIALFIAPVLVFASLLVGAPYLDLIFSSFEIAAVAFSGIV
                     LGFIALDGRSNWFEGAQLVGAYCIMAVSFFFL"
BASE COUNT          860 a         1963 c         1906 g          894 t
ORIGIN      
        1 cggaaagtaa gtgatgatcc gagcgcctac cagatcatga tcgtcagcca gggactcgta
       61 cgacgggtca tgctcttcaa gtttccgagc aagacgcgaa cggtccttgg ttcgcgcttt
      121 gatcgagtga atcgcaaagg ggtattggca cagcagatcg tggagcaatc tctccaaccg
      181 aggagtcaac gcattcaggg tcggaagctg gtcggcgtat tccgcaagga aagcattaag
      241 cgtcttctcg tccacactcg cagcttatgt gctgacaccc tcgctaggcg ctctctcggt
      301 gctcctcgta gatcgtcagc accgtgttca ttcggtctag gaaaaggtct agcagggccg
      361 ggtcgaagtg cttcccagac tctttgcgca tcatctctag tacgtggccc agcgggaacg
      421 ctcggcggtg cggccgagag tgcgtcagcg cgtcgaagac gtctgctatc gctgctatcc
      481 ggccctcgat cgggatcgaa tctccggcca ggccgcgcgg atagcccgtc ccgtcgtatt
      541 tctcgtggtg ggagcccgcc actatcgcgg ctatctgcac caccggcgag tccgagccct
      601 ccagcagtcg caccccgatc ggcacgtgct cttccatcat cctgcgctcg gtcggggtca
      661 ggatcccctc cttcagcagg atcaggtcgg agaccccgat cttgcctatg tcgtgcagac
      721 ggctcgccag cctcagcgtc tccgacgtct cggagtcggc accgttcgct cgggcgagga
      781 gcgcgcagta gcggctcatc cgctcgacgt gcccgctcgt ctcgtcgtcc cgatactcgg
      841 ccgccatcga gagccgcgac agcgtctcct cctgcgcggt ctgcaggtgc ttgcgcgcct
      901 cctcgagccg catcaacgag tcccacagct cgctcgtgcg ccggcggatc tcctgctgcg
      961 cggtatgctc ctggtgcgac acccggcgcg ccagcgacgc tgcctcgatc aggttgccga
     1021 cgcggattcg cacctcgtcc tcgtcgagcg gcttgccgat gaagtccttc gccccgaggg
     1081 cgagcgacgc ggatcgggcc cccgcatccg acggggccgc cactaccgcc accgagggac
     1141 tggacccatc cgaggggcag agcgcgcgca gcgcgtcgag ccccgcgtac tcggggcccg
     1201 tcaggtcgat caggacgacg gcgggctcga ccgcccggca cagctcggcc ggtgcggtca
     1261 agtcgttggc tacgtggagg tcctcgtacc cggcgctccg gagggcggcc tccaagcggc
     1321 ccgcgtcttc cggctcgccg agcaccagga cgcgggcatc gctggtctgc atcccctcac
     1381 ctatgagtcg cggtcggagc gtcgatgagg gccaacgatc ggccggcccc ccagccggcc
     1441 gatcgcggac ctcgtgctac gaagtaactg cgtggcacga gcatcttaac cacagaaggc
     1501 ggctcgtaca cccctcagag ggtgaaaaat ccgcgaacgg ctagagccgg accgggcggg
     1561 gcggcatcaa ggtgaaccgg atatgcggct cgacgaggac ggatgcgggc gaaaggtact
     1621 cggcggggtc gaggatcccg aggcgcgtcc acagatcgaa gcggccctcg aggagccgcc
     1681 ccgagaacgg gcgcagcctg cggtggtcga tccggccgtg cgtgtagaag ccgaaggggt
     1741 tgcgcgagaa cccgtgcagc cggtagccca ggaactggac gtgctcgtcg gcagtagtga
     1801 acgggtggat cggcgccgga gcgtccgtcg cctccgccag ccacgcgacc tcctcggccc
     1861 ccgaccgcgc ggtgcacgag taccggtggt acccgcggcc gtccttgtgc atgtcgtgct
     1921 cgaagctcgc catcttcgtc cccgtcgcca tcgcgccctg ccacagccac gagccgatcg
     1981 tgtcgcagta cgtcccgaag aagtagagcc cgcgcgcctc gccgtacgtc acgtaggtcc
     2041 ggaacgtgac ctggttgaac gtgtgggtgg ggcggttcgt gagccgcggg cggaggtcgc
     2101 ggttgcagaa gcacgcggtc gtgacgaagc acgtctcgcg tccgtcggcg ccctcgaagg
     2161 tctcgagcga gaccttgtcc gggagctgcg gcttcacccg cgccgcgggg accgcgtggg
     2221 tgatcaggat gaagttctcg aggtcgacgt cggcctcgac tgccttcggc ccccgggccg
     2281 gacgctgctc ctcccccggc gcggtcgtga tgtgcaggct ccccgtcacg ccgcgatcat
     2341 cgcgtgagcg cgccgaggta cttcccggcc gcgccggtgt tgtagagcac gacggggccg
     2401 cgcagctcgc cgagatgcgc gagcccggcg agcgtcgacg ctccctccgg gcagacgagg
     2461 atcccttcgg ccgcggcgag gtcgcgcacc gcagtgacga tctcgtcgtc ggacacggcg
     2521 accatcgtgc cgccgctgcc gcggacgacc ttcaggacga ggtcggcttc cgagggagcc
     2581 gggacgcgca ggcccgctgc gatcgtgtcg ggctccgcgg cccacggctc ggctgcgccg
     2641 gctccggctt cgaaggcgct gacgatcggc gcgcagccct cggcctggac gccggcgatt
     2701 acgggacccg aaccctcgta ccagccgagc gccgtgacct cttctacggc cttcgagcac
     2761 gccaccgcgg ccactccccc gccgaccgac gttacgacgg tgcccggtag gcggccgagc
     2821 tggtcgaagc actcgagcca cgcgctcttc tttccttcga cgcggtaggg ctcggcgaac
     2881 gtcgcggcga ggaaggctcc ggtctcgtct gcgatcgcgc gggcgcgccc cgcggcgtcg
     2941 gcgagcgtgc cctcgacctc gacgagctcg gcccccgcga gacggacctc gttcttgttc
     3001 tcctcggggg cgctgcgcgc catcgtgacg gtcatcgtca gcccggcccg ggccgcgtag
     3061 agcgaccacg ccgcgcccgc gttgcccgcg ctcggcatga cgacggactc ggcgccgagc
     3121 tcgaccgcgc gcgacagggc cgcggccgca ccccgggcct tgaacgtccc gctcggaaga
     3181 ggaccgtcgt ccttgacgaa gacctcgacc ccgaatcgct ctgagagccg cggcgcggag
     3241 gtcaggggcg tcggcccctc tccgagcgac accggctcgc cccgcaccgg aaggagctcg
     3301 cggtagcgcc acatgcccgg cgggcgagcc cggacctcgg cgaggtcgag cggcgacatg
     3361 tcgtagcggg cgagcagcgt cccgccgcat ccgcagcggt tctgcaggac gtccgcgtcg
     3421 tgggcgcgcc cgcagcgggc gcattcgagg tgggttaggc gggagatcgg actagacgaa
     3481 gccgagggcg gacttggcga gcgggtccat cttctcgggc gtccacggcg ggtacatgac
     3541 catctcggtc gtgacctcgc cgatgccctc gatgtaggcg gtggctgcct tgatctcctc
     3601 gtcgagcatc gggccgatcg ggcagcccat cgtcgtcaga gtgaactcga ccttgacgtt
     3661 gtggtcgtcg tcgacggcga cctcgtagac gaggccgagg tgccagatgt cgatcccgag
     3721 ctcgggatcg ttgacggtct tgagggcctc gacaacctct tcgcggatct cttccttggt
     3781 aggcatctca cccactgtaa cggggaggtg cgcgctgcta tttcccgcca cgcccgcggg
     3841 cgagccgggc gtccgggacg tagtcgtggt aggcggcccc cgcctcggtc ggcgagacca
     3901 tcctcgcctg ccagtggaac cgcctggacg gtggcagcgc cagcagggag ggcgggatcg
     3961 tcacctcgat cgagtccggg tccggtcgcc ggaccagcgc atagccgagg aactcctcgc
     4021 ggaccgagaa tgggttcggc tccgcctccg ggaacctctt cccgcgggtg accgcggcga
     4081 agggcgtgaa cccgtcctgg tcgacggagt caccccgcac gtcgacgtag cagaggcgct
     4141 cgaacgcatt gtcggagtcc gtgcttatac cgatggcgac tgcgtaatcc ttgccttcca
     4201 cgtgctcgag atccgcgttc gagaagggct cgtaggtgtg cagcctgaac gcgacctgat
     4261 ttcccgtgat ggcagccgga cgcgcggccc gtgcgcgctc tacgtcgagg acgactcgcg
     4321 ggccctcgcg cgggtcttcc accccgggca ggtccgcgac gttctggctc gtatcgccgt
     4381 gccacagcac gccagccgag gcgcagagcg ccaccaccaa caagcgcttc atgacgggga
     4441 cgacgctcga ggcccctctg ccgttcccgt actacgtcag ggccttgccc aaggccccgt
     4501 ccgcgtcggc cggcgtcgct tctctgatcg tgacgtcaga catccaggcc gagcaggcgt
     4561 gccgcgttgt tcttcagcac gtcttcctcc ttgtcgccca ggcccatctc gtcgaactcg
     4621 tcgaggatcc ggcccgggtc gagcatcggg tagtcgctcc cccacacgaa cctgtccgac
     4681 aggcggcccc gggcgtcccg cttgacctcg gccgggatgt acttcggcct ccaccccgac
     4741 aggtcgatcc acacgttcgt cttgtgcatc gcgctcgcga gcacctcgag gtgccacggg
     4801 tagccgaagt gcgccatcac gacggtgagc cccgggtggt gcgccgcgac catgtccatg
     4861 agcatcgggc ggccgtagtc gaacgcgatc ccggacccgc cgggcacgcc ggcgccgagc
     4921 cccgtctggc cgacgtgcac gacgatcggc agcgcgtgct cctcgcactt gtcccacagc
     4981 gggtcgtact gcggatcgtt gggatagaac gcctggacct gcgggtggat cttcagcccc
     5041 ttcatcccga gctcgacgac cgcgcggtcg agctcctcga ccgcgtcggg cttgtgcggg
     5101 tcgacgctcg cgaatccgac gaactgctcg gggaactcgc ggcagatctc ggcgacgcgg
     5161 tcgttcgtca gcggcgggag gccggtcgcc gtctcggcgt cccacgcgag caggactccg
     5221 agcaggtccc actccttgta ctcggcggcc atctcctccg cggtgcggac gcgcacctcg
     5281 gaacggaagt acttcgccgt gacctcgagc atcggcccga tcgaaccctc cagccattcg
     5341 gccctcgaga cgtggacgtg gaagtcgatc gcgcggggca agagggcctc cttagaggaa
     5401 gaagaacgac acggccatga tgcagtaggc gccgacgagc tgggccccct cgaaccagtt
     5461 ggagcgcccg tcgagcgcga tgaagccgag cacgatcccc gagaaggcga cggcggcgat
     5521 ctcgaagctc gagaagatca ggtcgaggta gggcgcgccg acgaggagcg acgcgaacac
     5581 gagcaccggc gcgatgaaca atgcgatctg cgtcgacgag ccg
//
LOCUS       DASQDO010000057         7169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_924827, whole genome shotgun sequence.
ACCESSION   DASQDO010000057 DASQDO010000000
VERSION     DASQDO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924827"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            450..1982
                     /gene="rny"
                     /locus_tag="VEV43_01880"
     CDS             450..1982
                     /gene="rny"
                     /locus_tag="VEV43_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HYP22297.1"
                     /translation="MEIVIGLVLGLLVGAVAGYAYRQSVAQKEVTSAEARARTVLADA
                     QKEAEAAKREALVEAKDEIFRMRSEAEQDLKRRGVEIDRKEERLAGRESNLDSRSESL
                     DRKERAIETREGELKAAQQELDRLADRARAEIERVAGMTQNDAKQALIEQIEDEAKRE
                     AMVIVRDVEAKAREEADKRARKIIAISMQRVASDLTTESTVQTVTLPNEDMKGRIIGR
                     EGRNIRAFEAATGTNLIIDDTPEAVVLSCFDPIRREMARLTLEKLISDGRIQPARIED
                     IHEKSRAEVEREIREGGEWAVLETGITDMHPELVRVVGRLKFRTSYGQNVLKHSVEAS
                     HIAGMIAAELGTDIALAKRCTLLHDIGKAVTHEVEGSHAIIGAEIAKRLKESPAVCHA
                     IEAHHGEVEQRTIEAVITQTADAISGARPGARRETLETYVKRLERLESIAMEFSGVDK
                     VYAMQAGREVRVMVKPHDVDDLQAEVVARDIAKKIEEELQYPGQIRITVIRETRTSAY
                     AK"
     gene            complement(1995..2636)
                     /locus_tag="VEV43_01885"
     CDS             complement(1995..2636)
                     /locus_tag="VEV43_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22298.1"
                     /translation="MRRSVGLLCVAAAFAVPAQVAAAPLPAPEPGAVADESALASWMF
                     PTDRPRHSKWIFAGAYRDATAGGRTITTGFAVKGSCKVVRERGETVTRCHGRGIGGPL
                     AESAFVVDPAQRTARLVMREDGVRHAIEWQADAPPAFPSGYFAGEACDEGAGQGAGFM
                     RHAAAHGDVFGRNVSRDGIDHALLSRGAMLTECTGRDLARRAAAGETIRVTFR"
     gene            2731..4131
                     /gene="miaB"
                     /locus_tag="VEV43_01890"
     CDS             2731..4131
                     /gene="miaB"
                     /locus_tag="VEV43_01890"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYP22299.1"
                     /translation="MSQAPSTYYIRTFGCQMNEHDSERMAGMLEAEGYSPVTAPEEAG
                     VVVFNTCCIRENADTKLYGNLGHMKALKDRHPGLKIVVGGCLAQKDRASIQQRAPYVD
                     VVLGTHNLASLPRLLAESSEGPAFEILEQTETFPSALPARRMSPWHAWVSISIGCNNS
                     CTFCIVPAVRGEEISRRMGDIVHEVEGLVADGVVEVTLLGQNVNSYGRDLDGTPLFSK
                     LLYALDEIEGLRRVRFTSPHPKDFRADTVRAMAECRTVCEHIHLPLQSGSDRVLKTMK
                     RAYSRRRYLDKVGMVRDAIPGVAITTDIIVGFPGETEEDFVQTLEVVEESQYDGAFTF
                     QYSPRPMTEAASFPDQLPKEVVQERYDRLVELQDAISFAKNRAEVGAVAEVLVEGPSK
                     KDPGKLTGRTRKNKLVHFPSDGADEGSFRTVTITGAHPHFLTGELTGDAAPARRGRSL
                     SLPLVTASSPGCTSCN"
     gene            4164..4811
                     /locus_tag="VEV43_01895"
     CDS             4164..4811
                     /locus_tag="VEV43_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22300.1"
                     /translation="MPEIGGAGTAATKATAGLDLAGAETIVIASPHSTRTGVYKAARG
                     DLDAFGPRGIDVSAETDAEAVEALAARWGRPVLDAPADHGIVVPLRLIEPSVPVVTVA
                     FAEGADAAEPEGLADAVSSLGGAVAFVASANLSAGLTERSPLPSLPGAAEADAAVLDA
                     LRQDPAALAHVDFESAGSCAAPVLATFGSLFAGHPCDVLAYEHPFGVGYAVARTR"
     gene            4808..5704
                     /gene="miaA"
                     /locus_tag="VEV43_01900"
     CDS             4808..5704
                     /gene="miaA"
                     /locus_tag="VEV43_01900"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HYP22301.1"
                     /translation="MKVAAIVGPTAVGKTEVSLRVAAELGAEIVSVDSMQVYRGMDVG
                     TAKPSRDEMERVRHHLIDLRDADHELTVAEFQELGRAAIEDVTRRGNLPLLVGGSGLY
                     FRALVDDLEFPPRSEEVRRNLETEAEEVGAEALYARLRDADPLAASRIEPGNVRRIVR
                     ALEVIEITGARFSDNVTWERYESRYDLAVAGLERERDELHRRIERRVDAMLAGGLLAE
                     AEAIRKSGAGRTARQALGYRQILEGGGRDEIAQATRRFARRQLSWFKADPRVRWFDAS
                     EPGLEDRLVGYFREALALPSRA"
     gene            5701..6531
                     /gene="dapF"
                     /locus_tag="VEV43_01905"
     CDS             5701..6531
                     /gene="dapF"
                     /locus_tag="VEV43_01905"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYP22302.1"
                     /translation="MRFAKYQGIGNDFVMFADPDDRLRLTPEDVRALCDRRFGIGADG
                     VIRVAPGTGGGDFFMDYVNSDGSLGEMCGNGIRCLAVFAAAEGLTDKNELKVETRAGL
                     KTITVADGGIRVDMGPPRFRPDDVPVLWQGDDALHAQVELDDQVVEAACLSMGNAHAV
                     LLVDDLASAPPTDLGPRIETHPMFPAGTNVEFVVVESAQRIEMSVWERGSGQTLACGT
                     GACAAAVAARLLRGTEDRVTVAMPGGELTVEWAGSISDVQPVFMTGPAVKSFEGDVEL
                     "
     gene            6531..>7169
                     /locus_tag="VEV43_01910"
     CDS             6531..>7169
                     /locus_tag="VEV43_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYP22303.1"
                     /translation="MRAAKRIEKLPPYLFAEIDRKVAEVKARGEDVISFGVGDPDLPT
                     PSHVVEALARASSDPATHRYPSYTGMPAFREAIASWYERRFGVRLDADEQVQPLVGSK
                     EGIFHLPVAFVDPGDVALIPDPGYPVYETGTILSHGEPLLLPLTPENGYVPDLDAIPE
                     DKLERAKVLWLNYPSNPTAACVDVDVFQRAVDFCRAHGLLLAHDAAYTEITFD"
BASE COUNT         1143 a         2520 c         2544 g          962 t
ORIGIN      
        1 cggcccccgc ggcggatctg aatccgcacc cggcgccgct gcgccctcga cctgcccgtg
       61 gccccactgg agccgctcat cgaagccccc cctctcgtcc ccgcgacgct acggaaccgg
      121 gcagaccggg ggaatacgac gaagtgccta gggacccccg cctcctgccc atacatgggt
      181 gggtgcattc cgcctcgccc cggcgtacca ttggactaga cacacacaat tcagtcccga
      241 cggtacgaac ggaccaacga aagttcccaa actcttcatg acgacgaccg aacgagaacc
      301 cgcacgcatc cgggccgggg gagccgaggc tccgccgggc cgaatgtctt ctcgtatccg
      361 ttagccgcca ggctttcgta gttagctcgt gccgtgtgcc agcccggctc ttcgccgtgc
      421 cggcgcccgt atgactaaag gagcgaggca tggagatcgt catcggtctc gtcctcggcc
      481 tcttggtggg agcggtcgcc ggctacgcct accggcagtc ggtcgcgcag aaggaagtca
      541 cgtcggcgga ggcccgcgcc cgaaccgtgc tcgcggacgc ccagaaggaa gccgaggcgg
      601 cgaagcgtga agctctagta gaggctaagg acgagatctt ccgcatgcgg agcgaggccg
      661 aacaggacct caagcgccgc ggcgtcgaga tcgaccggaa ggaagagcgc ctggccggga
      721 gggagtcgaa cctcgactcc cggagcgagt cgctcgaccg caaggagcgg gcgatcgaga
      781 cgagggaggg cgagctcaag gccgcccagc aggagctcga ccgcctcgcc gaccgggccc
      841 gggccgagat cgagcgggtc gccgggatga cgcagaacga cgccaagcag gcgctgatcg
      901 agcagatcga ggacgaggcc aagcgcgagg cgatggtgat cgtccgcgac gtcgaggcga
      961 aggctcgcga ggaggccgac aagcgggccc gcaagatcat cgcgatctcg atgcagcggg
     1021 tggcgtccga cctcacgacg gagtcgaccg tccagaccgt gaccctgccg aacgaggaca
     1081 tgaagggccg gatcatcggc cgcgagggcc gcaacatccg cgccttcgag gccgcgacgg
     1141 ggacgaacct catcatcgac gacacgcccg aggccgtcgt gctctcgtgc ttcgacccga
     1201 tccgtcgcga gatggcgcgg ctcacgctcg agaagctgat ctccgacggc cgcatccagc
     1261 cggcccgcat cgaggacatc cacgagaagt cccgcgccga ggtcgagcgc gagatccgcg
     1321 agggcggcga gtgggccgtg ctcgagaccg ggatcaccga catgcacccc gagctcgtgc
     1381 gggtcgtcgg ccgtctcaag ttcaggacgt cgtacgggca gaacgtgctc aagcactccg
     1441 tcgaggcctc gcacatcgcc gggatgatcg cggccgagct cggcaccgac atcgcgctcg
     1501 cgaagcggtg cacgctcctc cacgacatcg gcaaggccgt gacgcacgag gtcgaaggca
     1561 gccacgccat catcggagcc gagatagcga agcggctgaa ggagtcgccc gcggtctgcc
     1621 acgcgatcga ggcccaccac ggcgaggtcg agcaacgcac gatcgaggcc gtcatcacgc
     1681 agacggccga cgcgatctcg ggtgcacgcc ccggcgcacg ccgcgagacg ctcgagacct
     1741 acgtcaagcg cctcgagcgc ctcgagtcga tcgccatgga gttctcgggc gtcgacaagg
     1801 tctacgcgat gcaggccggc cgcgaggtgc gggtgatggt caagccgcac gacgtcgacg
     1861 acctgcaagc cgaggtcgtc gcccgcgaca tagcgaagaa gatcgaggaa gagctccagt
     1921 acccggggca gatccgcatc acggtgatcc gcgagacgcg cacctcggcg tacgcgaagt
     1981 aaggggccgc tcggctagcg gaacgtgacg cggatcgtct cgccggccgc ggctcggcgg
     2041 gcgaggtcgc ggcccgtgca ctccgtgagc atcgcccccc gcgacaggag cgcgtggtcg
     2101 atcccgtcgc ggctcacgtt gcggccgaac acgtcgccgt gcgccgcggc gtgtcgcatg
     2161 aagcccgcgc cctgaccggc gccttcgtcg cacgcctcgc cggcgaagta gcccgacggg
     2221 aacgcgggag gcgcgtccgc ctgccactcg atcgcgtggc ggacgccgtc ttcgcgcatc
     2281 acgagccggg cggtccgctg cgccgggtcg accacgaacg cggactcggc cagcgggccg
     2341 ccgatgccgc ggccgtggca gcgggtgacg gtctcgcctc gctcacggac gaccttgcac
     2401 gagcccttga ccgcgaagcc ggtcgtgatc gtccggcccc cggcggtcgc gtcgcggtac
     2461 gcgccggcga agatccactt ggagtggcgg gggcggtccg tggggaacat ccagctcgcc
     2521 agagcgctct cgtcggcgac cgcgccgggc tcgggagcgg ggagcggggc cgcggccacc
     2581 tgtgcgggga cggcgaacgc cgccgcaacg cagaggagcc caaccgatcg gcgcacccgc
     2641 cgcctccctc gtgaaccgca ccatcccgga cgaacgggga catcctaccc acgcaggctc
     2701 gcgaaagccc ggtgctacgc tcgccggacg atgagccagg ccccgtccac gtactacatc
     2761 cgcacgttcg gctgccagat gaacgagcac gactccgagc ggatggcggg catgctcgag
     2821 gccgaggggt acagcccggt gacggctccc gaggaagccg gcgtcgtcgt gttcaacacc
     2881 tgctgcatca gggagaacgc ggacacgaag ctgtacggca acctcggcca catgaaggcg
     2941 ctcaaggacc ggcaccccgg tctcaagatc gtcgtcggcg gatgcctcgc gcagaaagac
     3001 cgtgcctcga tccagcagcg cgcgccgtac gtcgacgtcg tgctcgggac ccacaacctg
     3061 gcgagcctgc cgcggctcct cgcggagtcg tccgaggggc cggcgttcga gatcctcgag
     3121 cagaccgaga cgttcccgtc ggcactgccg gcgcggcgga tgtcgccgtg gcacgcgtgg
     3181 gtgtcgatct cgatcgggtg caacaactcg tgcacgttct gcatcgtgcc ggccgtgcga
     3241 ggcgaggaga tctcgcgccg tatgggcgac atcgtccacg aggtcgaggg gctcgtcgcc
     3301 gacggggtcg tcgaggtcac gctcctcggt cagaacgtca actcctacgg ccgcgacctg
     3361 gacggcacgc cgctgttctc gaagctcctc tacgcgctgg acgagatcga gggcctgcgg
     3421 cgcgtgcgct tcacgagccc gcacccgaag gacttccgcg cagacacggt ccgggcaatg
     3481 gcggagtgcc gcacggtgtg cgagcacatc cacctcccct tgcagtccgg gtcggaccgc
     3541 gtgctcaaga cgatgaagcg cgcgtactcg cgccgccgct acctcgacaa ggtcgggatg
     3601 gtccgcgacg cgatacccgg cgtcgcgatc acgaccgaca tcatcgtcgg cttccccggc
     3661 gagaccgaag aggacttcgt ccagacgctc gaggtcgtcg aggagtcgca gtacgacggc
     3721 gccttcacgt tccagtactc gccgcgtccg atgaccgagg ccgcgtcgtt ccccgaccag
     3781 cttccgaagg aggtcgtgca ggagcgctac gaccggctgg tcgagctgca ggacgcgatc
     3841 tcgttcgcca agaatcgggc cgaggtcggc gcggtcgccg aggtgctcgt cgagggcccc
     3901 tcgaagaagg acccggggaa gctcaccggg aggacgcgca agaacaagct cgtgcacttc
     3961 ccgtcggacg gggccgacga aggatcgttc cggaccgtga cgatcacggg cgcgcacccg
     4021 cacttcctga cgggcgagct caccggcgac gcggcccccg cgcgccgggg acggtcgctg
     4081 tcgttgccgc tcgtcacggc atcgagcccg gggtgcacta gctgcaactg atcggcgcgg
     4141 tcgtcccgca cgcgccgctg ctgttgccgg agatcggcgg cgccgggacc gcggcgacga
     4201 aggcgacggc cgggctggac ctcgccgggg ccgagacgat cgtgatcgca tcgcctcaca
     4261 gcacgcgaac cggcgtctac aaagcggccc gaggggacct cgacgcgttc ggcccccgtg
     4321 ggatcgacgt gtccgcggag accgacgccg aagctgtcga ggcccttgcg gcccggtggg
     4381 gacgtcccgt cctcgacgcc ccggccgacc acggcatcgt cgtcccgctg aggttgatcg
     4441 agccgtccgt gcccgtggtc accgttgcgt tcgccgaggg cgccgacgcc gcggaacccg
     4501 aggggctcgc ggatgcggtc tcgtccctcg gcggggcggt cgcgttcgtg gcgagcgcga
     4561 acctgtcggc gggcctgacc gagaggagcc cgctcccgtc gctgccgggg gccgccgagg
     4621 ccgacgcggc cgtcctcgac gcgctgcgcc aggatcccgc cgcgctggcg cacgtggact
     4681 tcgagagcgc cgggtcgtgc gcggcccccg tcctcgcgac gttcggctcg ctcttcgccg
     4741 gccacccttg cgacgtcctc gcgtacgagc atccgttcgg cgtcgggtac gcggtagccc
     4801 ggacgcgatg aaggtagcgg cgatcgtcgg cccgaccgcc gtcggcaaga ccgaggtctc
     4861 gctgcgggtc gcggcggagc tcggcgccga gatcgtctcc gtcgacagca tgcaggtcta
     4921 ccggggcatg gacgtcggca ccgcaaagcc gtcgcgcgac gagatggagc gcgtccgtca
     4981 tcacctgatc gacctgcgcg acgcggatca cgagctcacc gtcgcggagt tccaggagct
     5041 cggccgcgcc gcgatcgaag acgtcacacg ccgcggcaac ctcccgctgc tcgtcggcgg
     5101 gagcggcctc tacttccgcg cgctcgtcga cgacctcgag ttcccgccgc gttcggagga
     5161 ggttcgccgc aatctcgaga ccgaggccga ggaggtgggg gccgaggccc tctacgcgcg
     5221 gctccgcgac gcagacccgc tcgcggcgtc gcggatcgag cccggcaacg tccgcaggat
     5281 cgtgcgcgct ctcgaggtga tcgagatcac cggcgcgcgc ttctcggaca acgtcacctg
     5341 ggagcggtac gagagccgct acgacctcgc ggtcgcgggc ctggagcgcg agcgggacga
     5401 gctccaccgg cggatcgagc gacgtgtgga cgcgatgctc gccgggggtc tgctggccga
     5461 ggccgaggcc atccgtaaga gcggcgcggg ccgcaccgcg cgccaggccc tcggctaccg
     5521 ccagatcctc gaaggcggcg gacgggacga gatcgcgcag gcgacgagga ggttcgcccg
     5581 ccggcagctg tcgtggttca aggcggaccc ccgcgtgcgg tggttcgacg cctccgagcc
     5641 cggcctggag gaccggttgg tgggctactt ccgggaggct ttggccttac cctctagagc
     5701 atgaggttcg ccaagtacca gggcatcggc aacgacttcg tgatgttcgc cgaccccgac
     5761 gaccggctcc ggctcacgcc ggaggacgtg cgcgctctct gcgaccggcg cttcggcatc
     5821 ggcgccgacg gcgtgatccg cgtcgcgccc gggaccggtg gcggcgactt cttcatggac
     5881 tacgtgaact cggacggctc gctgggcgag atgtgcggca acggcatccg ctgcctcgcg
     5941 gtcttcgcgg cggccgaggg cctcaccgac aagaacgagc tgaaggtcga gacgcgcgcc
     6001 ggcctcaaga cgatcacggt cgccgacggg ggtatccggg tcgacatggg cccgccgcgg
     6061 ttccggccgg acgacgtgcc ggtcctgtgg cagggcgacg acgccctgca cgcgcaggtc
     6121 gagctcgacg accaggtggt cgaggccgcc tgcctcagca tgggcaacgc gcacgcggtc
     6181 ctgttggtcg acgacctggc gagtgccccc ccgaccgacc tcggcccccg catcgagacg
     6241 cacccgatgt tccccgccgg gacgaacgtc gagttcgtcg tcgtcgagtc ggcccagcgg
     6301 atcgagatga gcgtgtggga gcggggctcg ggccagacgc ttgcgtgcgg caccggtgcg
     6361 tgcgcggcgg cggtcgcggc gcggttgctc cgcggcacgg aagaccgcgt gacggtcgcg
     6421 atgccgggcg gcgagctgac ggtcgaatgg gccggatcga tctccgacgt acagccggtc
     6481 ttcatgacgg gccctgcggt caagagcttc gaaggggacg tggagctgta gtgcgggccg
     6541 cgaagcggat cgagaagctc ccgccgtacc tgttcgcgga gatcgacagg aaggtcgcgg
     6601 aggtgaaggc gcgcggcgag gacgtcatct cgttcggcgt cggcgacccc gacctcccga
     6661 cgccgtcgca cgtcgtcgag gcgctcgccc gcgcatcttc cgaccccgct acgcaccgct
     6721 acccctcgta cacggggatg cccgcgttcc gcgaggcgat cgcgagctgg tacgagcggc
     6781 gcttcggcgt gaggctcgac gcggacgagc aggtgcagcc gctcgtcggc tcgaaggagg
     6841 ggatcttcca cctgccggtc gcgttcgtgg accccggcga cgtcgcgctg atccccgacc
     6901 cgggctatcc cgtctacgag accgggacga tcctgtcgca cggcgagccg ctgctgctcc
     6961 cgctgacgcc cgagaacggc tacgtgccgg acctggacgc gatccccgaa gacaagctcg
     7021 agcgcgccaa ggtcctgtgg ctcaactacc cgtcgaaccc gaccgccgcg tgcgtcgacg
     7081 tcgacgtctt ccagcgcgcc gtcgacttct gccgtgcgca cggcctcctg ctcgcccacg
     7141 acgcggcgta caccgagatc acgttcgac
//
LOCUS       DASQDO010000058         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_68254, whole genome shotgun sequence.
ACCESSION   DASQDO010000058 DASQDO010000000
VERSION     DASQDO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68254"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3123
                     /locus_tag="VEV43_01915"
     CDS             <1..3123
                     /locus_tag="VEV43_01915"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HYP22304.1"
                     /translation="PQDQAARDAAIKGVTIVSDKTSGTGTSLPEGMPRRVTVLSDDQK
                     DRWRWRVDPTPEGLTVTFANPAVPTGDYTLTVQGEGTDGLPVTLRNVTRGRWNRVDAD
                     DDGDFTLTLKARPGDRLELLRNRKSIAYVATLGVGVEVVDVNAFYNEAPEEPTNTPES
                     DVLGIYTGYGDPTLSLCGAPVSDIASTLDLGTLFDASPGYPHPITVVALIGFRGLALL
                     HSPIDNVGSLRFFNESCLNVDGSAQVSGLDVVEDYPIDFDEDGELEESEERDYVLVSH
                     RGKGLFIFDATDREELVPVGRVTLPGQAAHVGFDRERRKIYVSAHGAGIFVVDFDELP
                     GRESMIDINGDGTDDRVLETIPLPNANNNSPVFLVPELGVAYAGGLNRGLTSIEVGTP
                     LLATVAEEREQVVPAPDPAAIERPRLRPVGRLAPLGVPTAPESFGGGGPELSASFRVL
                     ATLPAVVGAETKVDVVSLGPGGLPISGAGDADVIADLPRPALEGENGVVLRRLADQPW
                     EDGYQLYLSKEIAVLADLRAAKAYQRTAKEDEQCVRCDQEELHIQIDALEILSGTKIA
                     VRLPAELRQKLGKMYDDARLDASEITVESVPWEVAPAVRQEPQLNPSMGMGDVAPGTL
                     LHSGEMTHTASDLFIKGRKLDFAFKRVYRNQTVGSGPLGPGWDHVYNMRLRRLPNRDV
                     EYYDGRGRRVLFKRQENGTLEAPTGMFVTLERTSAGWVMLDAGRNLYRFDGFGRLTAI
                     ADAFKDSTENGNEITFFYNLDSRLTRIVANHREVLLSYDSEGRLEEVRDFSGREVEYA
                     YDDAGRLERVTSPAITTGESQFPDGLTTAYSYETGAGALAAHLALRDNLKAVTDPKGQ
                     TWLELEFGDQDGDGRSEEVVLQRWGEDFVSLSYDLGPAGNTTATVTDRRGHDAVYAHN
                     VAGQVRSVQDRGGFTSTFEYDDEGIRTREVTPLGRRIESQPDATPALRRARGNISRMT
                     IVADSRGPNGSSSELVKLNQQFDESTNLPTVETDARGARKETDRNRDGLPERVTVQGD
                     GETPTAVTIY"
     gene            3217..>3826
                     /locus_tag="VEV43_01920"
     CDS             3217..>3826
                     /locus_tag="VEV43_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22305.1"
                     /translation="MTVDDIDLKLTTRYETDLRGNVTEIIDPRGVRHEYIYNEVDWLV
                     EERVAASGSSDGAPPLSLVTKYLYDENGNVIEKQEPFGEGGTISTRWSHGLLNEVRSV
                     DRELAPGGETAHESYEYDPNLNVTRAVDAEGRATSFAYDERDLVVTRTLESATDDPIT
                     ITYTYDAEGQQTQMTDGRGGLWKTEYDGYSRVKAQIDPAGTTT"
BASE COUNT          668 a         1344 c         1303 g          511 t
ORIGIN      
        1 ccgcaggacc aggcggcacg ggacgcggcg atcaagggcg tgacgatcgt ctccgacaag
       61 acgagcggca ccgggacctc gctgcccgag ggcatgccgc gcagggtcac cgtgctgtcg
      121 gacgaccaga aggaccgctg gcgctggcgg gtcgatccga cgccggaggg cttgaccgtc
      181 accttcgcga acccggccgt gcccacgggc gattacaccc tcacggtgca gggcgaaggg
      241 accgacgggc tgccggtgac gctccgcaac gtcacgcgcg ggcgctggaa tcgcgtcgac
      301 gccgacgacg acggcgactt caccctgacg ctcaaggcgc gtccagggga tcgcctcgag
      361 ctgctgcgca accggaagtc gatcgcctac gtcgcgacgc tcggcgtcgg cgtcgaggtc
      421 gtggacgtga acgccttcta caacgaggcg ccggaggagc cgaccaacac gccggaatcg
      481 gacgtcctcg gcatctacac gggctacggc gacccgacgc tctcgctgtg cggagcgccc
      541 gtttcggaca tcgcctccac cctcgacctt ggcaccctgt tcgacgcttc gccggggtat
      601 ccgcacccga tcaccgtcgt ggccctgatc ggcttcaggg gcctggcgct cctccacagc
      661 ccgatcgaca acgtcgggag tctgcgattc ttcaacgaga gctgtctcaa cgtcgacggc
      721 tcggcacagg tctcgggcct cgacgtcgtc gaggactacc ccatcgactt cgacgaggac
      781 ggcgagctcg aggagagcga ggagcgcgac tacgtgctcg tcagccaccg cggcaagggc
      841 ctgttcatct tcgacgcaac cgatcgcgag gagctcgtgc cggtcggccg cgtgacgttg
      901 ccgggtcagg ccgcgcacgt gggattcgat cgcgagcggc ggaagatcta cgtctccgct
      961 catggcgccg ggatcttcgt cgtcgacttc gacgagctcc ccgggcgcga gagcatgatc
     1021 gacatcaacg gcgacggtac cgacgaccgc gtgctcgaga cgatcccgct gcccaatgcg
     1081 aacaacaact cgccggtctt cctggtgccg gagctcggcg tcgcctacgc cggcggcctg
     1141 aaccgcggcc tgacgagcat cgaggtgggc acgccgctct tggccaccgt ggccgaggag
     1201 cgggagcagg tcgtgcccgc gcccgacccg gccgcgatcg aacggccgcg cctccgcccg
     1261 gtcggccgcc tggcgccgct cggcgtgccg accgccccgg agagcttcgg cggcggcggc
     1321 ccggagctgt cggcctcgtt ccgggtgctc gccacgctgc cggccgtcgt cggcgccgag
     1381 accaaggtcg acgtcgtcag cctcggtccc ggcgggctgc cgatctccgg cgcaggcgac
     1441 gccgacgtga tcgcggacct gccgcgcccg gcgctcgaag gcgagaacgg ggtcgtgctc
     1501 cgccgcctcg cggaccagcc gtgggaggac ggctatcagc tctatctctc gaaggagatc
     1561 gccgtcctgg cggacctgcg ggccgccaag gcgtaccagc gcacggcgaa agaggacgag
     1621 cagtgcgtcc gctgcgatca ggaggagctc cacatccaga tcgacgcgct cgagatcctc
     1681 tccgggacga agatcgcggt ccgcctgccg gccgagctgc ggcagaagct gggcaagatg
     1741 tacgacgacg cccggctcga cgcctcggag atcaccgtcg agagcgtgcc gtgggaggtc
     1801 gcaccggcgg tgcgccagga gccgcagctc aacccgtcga tgggcatggg ggatgtggcg
     1861 cccggcaccc tgctccactc gggcgagatg acgcacaccg cgtccgacct gttcatcaaa
     1921 ggacgcaagc tcgacttcgc gttcaagcgc gtctacagga accagaccgt cggctccggg
     1981 ccgctcggcc ccggctggga tcacgtctac aacatgcgcc tgcgacggct ccccaaccgc
     2041 gacgtcgagt actacgacgg ccgcgggcgc cgcgtgctct tcaagcggca ggagaacggc
     2101 accctggagg cgccgaccgg gatgttcgtc acgctcgaac ggacctcggc cggctgggtg
     2161 atgttggatg cggggcgcaa cctctaccgc ttcgacggct tcggacggct caccgcgatc
     2221 gccgatgcct tcaaggacag cacggagaac ggcaacgaga tcaccttctt ctacaacctc
     2281 gactcgcggc tgacgcgcat cgtcgccaac caccgcgagg tgctgctgtc ctacgactcg
     2341 gaagggcgcc tcgaggaggt ccgggacttc agcggtcgcg aggtcgagta cgcctacgac
     2401 gacgccggcc ggctcgagcg ggtgacctcg cccgccatca ccaccggcga gtcgcagttc
     2461 cccgacggtc tcaccaccgc ctacagctac gagaccggcg ccggtgcgct cgccgcacat
     2521 ctggcgttgc gcgacaacct caaggcggtc accgatccca aggggcagac ctggctcgag
     2581 ctggagttcg gggatcagga cggcgacggg cggtcggagg aggtcgtcct gcaacgctgg
     2641 ggcgaggatt tcgtcagcct gagctacgac ctcggtccgg ccggcaacac caccgccacg
     2701 gtcaccgacc ggcgcgggca cgacgcggtc tacgcccaca acgtcgcggg ccaggtgcgc
     2761 tccgtccagg atcgcggtgg cttcacctcg acgttcgagt acgacgacga aggcatccgg
     2821 actcgcgagg tgacgccgct cggccggcgg atcgagagcc agcccgacgc gacgccggcc
     2881 ctgcggcgcg cccgcggcaa catttcgcgg atgaccatcg tcgccgactc ccgtggcccg
     2941 aacggctcgt cgtcggagct cgtcaagctc aaccagcagt tcgacgagtc gaccaacctg
     3001 ccgaccgtcg agaccgacgc gcgcggtgcc cgtaaggaga ccgaccgcaa ccgtgacggc
     3061 ctgccggagc gggtgaccgt ccagggggac ggcgagacac cgacggcggt cacgatctac
     3121 tagtacaacg agcacggcca gccgaccctg atccagaacc cgaaccgcaa tgccacgctc
     3181 tacgagtacg acttcacttc gggttatctc aagtcggtga cggtcgacga catcgacctc
     3241 aagctgacga cgcggtacga gaccgacctg cggggcaacg tcaccgagat catcgatcca
     3301 cgcggcgtac gtcacgagta catctacaac gaggtcgact ggctggtgga ggagcgggtc
     3361 gcggcatcgg gctcgagcga cggcgcgccg ccgctctcgt tggtcaccaa gtacctctac
     3421 gacgagaacg gcaacgtcat cgagaagcag gagccgttcg gcgagggtgg gacgatcagc
     3481 acgcgctgga gccacggcct gctgaacgag gtccggagcg tcgaccgcga gctcgcgccg
     3541 ggtggggaga cggcgcacga gagctacgag tacgacccca acctgaacgt cacccgggcc
     3601 gtggacgccg aaggccgggc gacgagcttc gcctacgacg agcgcgacct cgtggtcacc
     3661 cgcacgctgg agagcgccac ggacgacccg atcaccatca cgtacaccta cgacgccgag
     3721 ggccagcaga cgcagatgac ggacggccgc ggcgggctgt ggaagacgga gtacgacggc
     3781 tacagccggg tcaaggcaca gatcgatccg gccggcacca cgaccc
//
LOCUS       DASQDO010000059         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_190903, whole genome shotgun sequence.
ACCESSION   DASQDO010000059 DASQDO010000000
VERSION     DASQDO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190903"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VEV43_01925"
     CDS             complement(<1..659)
                     /locus_tag="VEV43_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYP22306.1"
                     /translation="MRNDKRRWVVALMAALLLLGAACGGGEEGGEEEGTGTGTETEGD
                     AGAQVEEFPANTTMGKIQEKGELIAGVKYDVPPFGFVDPQTDEVKGFDVDVAQEIADR
                     LGVELNATEAISDNRIPFLKDGTVDIVASTMTFTTDRDVEIDFSEPYYIANGRILVGE
                     GTDDVETLADLKGKSACTALGSTYEGLLKEAGGIDIQLPDTYSECYELIQNGSVDAVV
                     T"
     gene            complement(697..1491)
                     /locus_tag="VEV43_01930"
     CDS             complement(697..1491)
                     /locus_tag="VEV43_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HYP22307.1"
                     /translation="MGADLSSDHAAGPSGAPRSNGNLVVLRNVTKSFGDNVVLDGIDL
                     SIDAGEAVVIAGPSGSGKSTMLRCVNALETIDSGEITFDGRSINNAGKDIYKIRADIG
                     MVFQSFNLFPHKTVLQNITLGPTEVKGVSDSDAKNRAKELLERVGIPEKADQFPADLS
                     GGQQQRVAIARALAMEPKLMLFDEPTSALDPEMIREVLDVMRDLAKSGMTMMVVTHEM
                     GFAREVCDRICFIDGGKIVEEGPPEEFFNNTKSERAKEFVDKIIHH"
     gene            complement(1539..1829)
                     /locus_tag="VEV43_01935"
     CDS             complement(1539..1829)
                     /locus_tag="VEV43_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYP22308.1"
                     /translation="MKRDADTGRSPSRSVAGADVTLKIPRPLYEQLKQVIEGSGFHSV
                     TEFAVYVLRDLVSHRGHPVVPPPVAEPAPDVDPLSPDEIEAIRQRLTSLGYL"
     gene            complement(1826..2971)
                     /locus_tag="VEV43_01940"
     CDS             complement(1826..2971)
                     /locus_tag="VEV43_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22309.1"
                     /translation="MDPATEVPEANAATETSPAPRPRARRSGPQRAVGAAWWILRFLL
                     ALFLFIGALQVMKEGAAGLSILSHDGLAKNAGATLGLGWIGALFVLSGSPIAASALAL
                     VKADTITEVEGFTMLTGSRLGAAFVVLLVAVIAALRSTEGKRMAPVSTAVMALSTTAL
                     IYVPGAVLGFFLLGWEPFHSLDLQFPAQFGDLIDVVYGDLLALVEDWSAPLLFLGGLA
                     LLLLAFKLIDSVVPELGDEAIESTRLSWLRRKWPMFGLGCLVAFVAMSVSVALTILVP
                     LVAKGYVKREDILPYIMGANITTLGDTLLAAFLLHSPAAVRIVLAGIIGTTIVSVILL
                     TFFYPQVRRGMWRFQRQMVKSKSRLAIFTAALFAVPLAIVGVSMVVA"
     gene            3091..>3331
                     /locus_tag="VEV43_01945"
     CDS             3091..>3331
                     /locus_tag="VEV43_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22310.1"
                     /translation="MSRRTRRLPRVVLAAAIVAAIFPAVPAGASAVSNGPAGPRIRTI
                     ESPRTMARSFTLDRAGAVSPEWPATHIGFSWKGDEG"
BASE COUNT          530 a         1096 c         1162 g          543 t
ORIGIN      
        1 tcggtgacga cggcgtcgac cgagccgttc tggatcagct cgtagcactc cgagtacgta
       61 tcggggagct ggatgtcgat cccgccggcc tccttgagca ggccctcgta cgtggagccg
      121 agggcggtgc aggcgctctt gcccttgagg tccgcgagcg tctccacgtc gtcggtgccc
      181 tcgcccacga ggatgcggcc gttcgcgatg tagtacggct ccgagaagtc gatctcgacg
      241 tcacggtcgg tcgtgaacgt catcgtcgag gcgacgatgt cgacggtccc gtccttgagg
      301 aacgggatgc ggttgtcgga gatggcctcg gtcgcgttca gctcgacgcc gaggcggtcg
      361 gcgatctcct gcgcgacgtc gacgtcgaag cccttgacct cgtcggtctg cgggtcgacg
      421 aagccgaacg ggggcacgtc gtacttgacg cccgcgatca gctcgccctt ctcctggatc
      481 ttgcccatcg tcgtgttggc ggggaactcc tcgacctgcg caccggcgtc gccctcggtc
      541 tccgtcccgg tgccggtccc ctcttcctcg ccgccttcct cgccgccgcc acacgcggcc
      601 cccagaagca gcagcgcggc catcagcgct accacccacc tccgtttgtc gttcctcatg
      661 cttccccctt ccgtgtcgtg cggtatctgc ccgcggtcag tgatggatga tcttgtcgac
      721 gaactccttg gcccgctcgc tcttcgtgtt gttgaagaac tcctcgggcg gtccctcctc
      781 gacgatcttg ccgccgtcga tgaagcagat gcggtcgcac acctcgcggg cgaagcccat
      841 ctcgtgcgtg acgaccatca tcgtcatgcc gctctttgcg aggtcgcgca tgacgtcgag
      901 gacctctcgg atcatctcgg ggtcgagggc cgaggtcggc tcgtcgaaga gcatgagctt
      961 cggttccatc gcgagggcgc gcgctatcgc gacgcgctgc tgctggccgc cggaaaggtc
     1021 ggcagggaac tggtcggcct tctcggggat gccgacgcgc tcgaggagct ccttggccct
     1081 gttcttcgcg tcggagtccg agacgccctt cacctcggtc ggcccgagcg tgatgttctg
     1141 gagaacggtc ttgtgcggga acaagttgaa cgactggaag accatgccga tgtcggcgcg
     1201 gatcttgtag atgtccttgc cggcgttgtt gatcgaccgg ccgtcgaagg tgatctcgcc
     1261 ggagtcgatg gtttcgaggg cgttgacgca gcggagcatc gtcgacttgc ccgagcccga
     1321 cggcccggcg atgacgacgg cctcacccgc gtcgatcgac aggtcgatgc cgtcgagcac
     1381 gacgttgtcc ccgaagctct tcgtgacgtt tcgtaggacg accaggttgc cgttggaacg
     1441 gggcgcgccg gaaggccccg cagcgtggtc ggaagaaagg tcagccccca tatgcaggcg
     1501 ggagtctagc ggaggaagcc cccggacgtt cgggatggtt agaggtagcc gaggctcgtg
     1561 agccgctgcc ggatcgcctc gatctcgtcg gggctcagcg ggtcgacgtc gggcgcgggc
     1621 tcggccaccg gcggcgggac caccggatgt ccgcggtgcg agacgaggtc gcgcaggacg
     1681 tagacggcga actcggtgac cgagtggaag ccggacccct cgatgacctg cttgagctgc
     1741 tcgtacaggg gccgggggat cttcagcgtg acgtcggcgc cggcgaccga ccggctcggc
     1801 gatcggccgg tgtccgcgtc ccgcttcacg cgaccaccat cgagacgccg acgatcgcca
     1861 gcggcaccgc gaacagggcc gcggtgaaga tcgcgaggcg cgacttgctc ttgaccatct
     1921 gtctctggaa cctccacatc ccccgccgga cctgaggata gaagaacgtc aggaggatca
     1981 cgctgacgat cgtcgtcccg atgatccccg cgaggactat gcgcacggcg gcgggcgagt
     2041 ggaggaggaa ggccgccagc aacgtgtcgc ccagcgtcgt gatgttcgcg cccatgatgt
     2101 acggaaggat gtcctcgcgc ttcacgtagc ccttcgcgac cagcggcacg aggatcgtca
     2161 gcgcgaccga caccgacatc gctacgaacg ccacgaggca acccaggccg aacatcggcc
     2221 acttcctgcg tagccacgac agacgtgtgc tctcgatcgc ctcgtcgccg agctcgggga
     2281 cgaccgagtc gatcagcttg aaggccagca ggagcaacgc aaggcccccg aggaagagca
     2341 gaggcgcgga ccagtcctcg acgagcgcga gcaggtcgcc gtacacgacg tcgatcaggt
     2401 cgccgaactg ggccgggaac tggaggtcga gcgagtggaa cggctcccac ccgagaagga
     2461 agaaccccag gaccgcgccc gggacgtaga tgagcgcggt ggtcgagagc gccatcacgg
     2521 cggtcgagac cggggccatc ctcttgccct cggtgctgcg gagggccgcg atcaccgcga
     2581 cgagcagcac gacgaaggcg gcgccgaggc gtgacccggt cagcatcgtg aagccctcga
     2641 cctccgtgat cgtgtcggcc ttcaccagcg cgagcgccga ggccgcgatc ggcgatcccg
     2701 acaggacgaa cagcgccccg atccagccga ggccgagcgt cgccccggcg ttcttggcga
     2761 ggccgtcgtg actgaggatg ctgaggccgg ccgctccttc cttcatcacc tggagcgcgc
     2821 cgatgaacag aaagagcgcc aggaggaaac ggagaatcca ccaggcggcg ccgacggctc
     2881 gctgggggcc ggagcggcgg gcgcgcgggc ggggggcggg agaggtctcc gtagccgcgt
     2941 tggcctccgg gacctcggtt gccgggtcca tcggggtctt atagcactcc tatgatagtg
     3001 acacaaggac ccaaggccgg agcgcgacgt tccggaggcg gggaacgttc tggagggcat
     3061 gggcgtggga ctagggtggc gtgcttcgtg atgagcagac gtaccaggcg acttccccgg
     3121 gtggtcctgg cggcggcgat cgtggcggcg atcttccccg ccgtgcccgc gggtgcctcg
     3181 gccgtgtcga acggtcccgc cggcccccgc atccggacga tcgagtcccc ccgcacgatg
     3241 gcgcggtcct tcaccctcga ccgagccggc gcggtctcgc cggagtggcc ggcgacccac
     3301 atcgggttct cctggaaggg cgacgagggg a
//
LOCUS       DASQDO010000060        10369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1169731, whole genome shotgun sequence.
ACCESSION   DASQDO010000060 DASQDO010000000
VERSION     DASQDO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169731"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..463)
                     /locus_tag="VEV43_01950"
     CDS             complement(263..463)
                     /locus_tag="VEV43_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYP22311.1"
                     /translation="MAQGTVKWFSNEKGFGFISQPDGEDVFVHFSAIQVDGYKSLEEG
                     QAVEFEVTQGAKGKQAANVRPV"
     gene            659..1000
                     /locus_tag="VEV43_01955"
     CDS             659..1000
                     /locus_tag="VEV43_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22312.1"
                     /translation="MSEDSDRRGRRTVPGVRELRELYEGAVGCKCMSCGKAEGRARTF
                     AASIPEDELSNLIVLCDPCDEARRGCSPVDPAFLSLIVAKTRRERSALGRALDPRNSR
                     RVLEYLRMRAL"
     gene            complement(997..2424)
                     /locus_tag="VEV43_01960"
     CDS             complement(997..2424)
                     /locus_tag="VEV43_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22313.1"
                     /translation="MARHATNRRRIAAALASLLLLAGCSDAAPPDATGATETPAPAAT
                     PAPEKTGGVSERSGKCGPPRAAPPEEVPHYDMSMRVDTDAGTVTGHSDVTFVADRPTS
                     RLVFRLWPNGPRLASEGTELAVEDARLRGGGGSVDRPDPTTLVFELDDPLPPGETVVA
                     ELDWTLDLPGPILDRISQDGDTIRLGSFFPILAWEGERGWATDPPTTTLAEASTSPTA
                     DFDVRIEVPQGLDVIASGRENGPGRWVAEDVRDFAVAVGRFDVTTARVDAPNPVQVTV
                     GVAEGLGSSKDDFRDAIARALRDLSKRYGPYPWRTFSMAIVPGLGRSGIEYPTMIFQG
                     DDSLTWATTHEVAHSWFYGLVGNNQGRDPWLDEGVTSWAQARGDGILGYFRDYEMRQA
                     ARGRLGKSMRFWDGHEGDYYDGVYVQGVKALDALGRARKVDCALRRYVARNAYEVATT
                     DDLVEALEESFPRASRVLKRFGARL"
     gene            2423..4012
                     /locus_tag="VEV43_01965"
     CDS             2423..4012
                     /locus_tag="VEV43_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP22314.1"
                     /translation="MGSIEVVNLTHRVPGGRTLLEEATFRAGDGDHVALVGANGTGKT
                     TLLRLIGGDEKVQSGAVRVDGVLAVMRQFVGIPGESLTVRDLMLQLSPAPLQAAARAL
                     ADAEAAIAEDGGKKSQLRYAAALAGWESAGGYTAEVLWDTCTTIAVKLPFHRVEDRPI
                     ATFSGGEQKRLALEVIFRSDATVVLLDEPDNFLDLQGKAWLEEQMRASQKTILYVTHD
                     RALLAATAHRIVTLEGNRVWTHGGGFETYHAAREQRMDRMEEQHRHYRQEHERLTALV
                     KEFKRKAAYNSDWASRAEAMEKRLRRYEKENEPPPRLVEQNVRVRVAGGRTGKIAFRA
                     RGLTLPGLVEPFDTEIHFGERVGILGRNGTGKSHFLRLLAGEDVGHEGTWMLGARVEP
                     GFFSQTHQHPEWDPKPILDLVMGTGLQRTPAMSALKRYELAADAQQPFSKLSGGQQAR
                     LQILMLEIASPTMLLLDEPTDNLDIASAEALEKGLDGYTGSVLAVTHDRWFMRVFDRF
                     LVFREDGVVEEALTPPLEKAG"
     gene            complement(4009..4872)
                     /locus_tag="VEV43_01970"
     CDS             complement(4009..4872)
                     /locus_tag="VEV43_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYP22315.1"
                     /translation="MAILLGLAAALTYGAGDFVGGLVTRRAPLFTVVLLSQVAGTALL
                     FAAVPFFVESGPTASALAWGGASGVAGSTGVLFLYRGLAAGRMSVVAPITSVEAAVVP
                     VVWGLATGERPPALALVGVVVALAAVVLVAGFEPRDVAEDEPDGPPRGRFAPGVADAL
                     VAGVAFGAFFILLDGAGDETGLWPLVAGRTASFTILTVLVLVRRERIHAAPGTRVPIV
                     AAGALDVAANLLYLLATREGLLSIVAVLTSLYPASTILLARIFLGERMGRLQVGGLAL
                     AAAGVVMMTAP"
     gene            4959..7397
                     /locus_tag="VEV43_01975"
     CDS             4959..7397
                     /locus_tag="VEV43_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYP22316.1"
                     /translation="MAVKAGRLFGGAASDVVRVRPGRRVAGAIALVLPIVVVLTAGFG
                     IIAIRQRADDRREAQVMLSRISASANRQRVLESNALELGLVAEGRPNATLARELRSLP
                     DELARLDDEVAQALTRLRAAEPGSTELDDLAVAVNRYQVSLEAQSALLTAGRFDESFG
                     YHRSRVDLAYRNVSQELAQQDARFDALTQDASRMADFGTLGILGFALVLLAFLQFDRA
                     RRAAAVDEAQRKVLSESEARFRSLVQNSRDVICIIDERGRALYASPAVAEVFGITPEQ
                     VCGEDLLLLAHPDERKRVRGSLAELAAEPRGASSKLECRVRHDDGSFKDLEVTATNLL
                     DDAYVGGIVLNARDISERKVAERDNEALQEQLAHQAFHDPLTGLANRALLRERLQHAL
                     ARVARSRAPLADMFIDLDEFKAVNDSMGHDVGDELLKAVAGRLGESVRPADTIARLGG
                     DEFAILLEGMFSAAEAPRVAERIIRTFTAPFDLGGHPVTIGASIGLALKDGKEGTEEL
                     LGNADVAMYAAKARGRGCYEVYRPELRLARTQRIRQELDLQTAVTRNEFVVHYQPIVD
                     MATGCMSGAEALLRWAHPTKGLIPPMDFVELAEETGLIVPLGRWILREACRNGAAWKK
                     THPEAAEMKVSVNLSVRQFQETDLVQQVADTLEVTRFPASDLVLEITESVFLNDSPAT
                     LAAIQGLKDLGVTIAIDDFGTGYSSLSYLRSFPIDMLKIDRSFIDGIDRGVEDAALAR
                     AIVQIGENLNLRIVAEGIETPEQLREVRRLGCHEGQGFFLGTPLDHDELKALLARGAT
                     RTNWLDDAPLEAVT"
     gene            7430..8029
                     /locus_tag="VEV43_01980"
     CDS             7430..8029
                     /locus_tag="VEV43_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209261.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HYP22317.1"
                     /translation="MVPVDPDLDELRSTAATCTACDLYRNATQTVFGKGLTRSQVMLV
                     GEQPGDQEDVAGEPFVGPAGRLLDEALVAAGIDRDQAYVTNVVKHFKFEQRGKRRIHK
                     KPNMAEITACRPWLDAEIEVVRPAALVCLGATAAQALLGRSFKVTQQRGEFVESDLAP
                     LVTATIHPSAILRAPDEREVEMKGLVDDLTKVRKALAKL"
     gene            8052..8312
                     /locus_tag="VEV43_01985"
     CDS             8052..8312
                     /locus_tag="VEV43_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22318.1"
                     /translation="MSKTARFFAGLRVADEDPADAFLRVTSYRGAEVIEAPEGTVEVP
                     ANHVRFSVWVGDRAHCVVSIPESEARELAAFLTAETHRVETV"
     gene            8357..9907
                     /locus_tag="VEV43_01990"
     CDS             8357..9907
                     /locus_tag="VEV43_01990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYP22319.1"
                     /translation="MPAQETERVVGSRYALEESIGRGGMGTVWRATDRLLQRAVAVKE
                     VQLPATVPEEERAVTRSRVLREARAAASISHPGAVIVYDVLEEEGRPYIVMELVEAPT
                     LEDVVRRDGPLDEERAARVGLEILGALEAAHAQGIVHRDVKPANVMLPQGRHAKLADF
                     GIASVKGDPRLTATGLVLGSPSYMAPEQASEDVTGPASDLWSLGATLYYAVEGEPPFD
                     RGQAIPTLTAVVGEEPRPLRKARALREVIGSLLEKDPAQRPSPEVLRRMLERALAGGS
                     TTEVAPPPTEVVTEPAEVATEPAPVEPAPVPAAPRARPWLAILAAAGLLLVVAVLLFL
                     SMGEDEGGAGNEAGRNRGAANERGDETDPEEPAEEAEPAGVVPELTEYEDPEAGYQVS
                     YPEGWDVEPLGDTRTDFTDPETGAYLRMDWTPTPGDDPVAAWEGQSASFAGRYADYEE
                     IRVDPVTFQGFPAAEWEYTYSGVHAINVGVVTDTYGYALNFQTRAEDWEESQALFEAI
                     KETFVPAP"
     gene            10004..>10369
                     /locus_tag="VEV43_01995"
     CDS             10004..>10369
                     /locus_tag="VEV43_01995"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HYP22320.1"
                     /translation="MGRIASARIHPAIGIARVGDSPDEYFVGPEVLRPRQEDAYKDAQ
                     GRLKRQAARFRIYGYDSDGELVGEITSADAEIVWEVHVANTKGAWYDFVVALDLTEAA
                     AVKSPRRNSWMGREGRDELV"
BASE COUNT         1590 a         3626 c         3750 g         1403 t
ORIGIN      
        1 ggggtcgttc ggcgtggtgg ggaagcggga gcctgcggtg gcccccggcg ggacggcgag
       61 cgcgactgcg gtggcgagcg tcagggctgc gccgatggcg ccgcggcgac cgagctggtg
      121 tgacatgagc tctcccctcc ggtgggccgg cccgtcacca cttcggaacg agaaagggcc
      181 cccgaggtaa cccgggccgg acgcgaaacg gcccccgggg gtcacccggg ggccgtcttg
      241 atcatcgttg ctgagggtcg ccctagacgg ggcgtacgtt cgccgcctgc ttgcccttcg
      301 cgccctgagt cacctcgaac tcgacggcct ggccctcttc gagggacttg tagccgtcga
      361 cctggatcgc ggagaaatgt acgaatacgt cttctccgtc cggctgcgag atgaacccga
      421 agcccttctc gttgctgaac cacttaacgg tgccctgtgc cactccacac tccttttccc
      481 ggccgagggg cgacaagctc tttcttgccc gtgctccccc cggcttgagc ggtacggacg
      541 ctaacacaca cggatgacgc agcgccggac agtcaccgcc ttccgagcaa cctcgggcgc
      601 cctcgcgtcg ttgtgtatgg ggagaacccg ttcggaccgt cctgggggga gccgacggat
      661 gtcggaagac agcgacagaa ggggccggcg caccgtgccg ggcgtgcgcg agctgcgtga
      721 gctctacgag ggcgccgtcg gctgcaagtg catgtcgtgc ggcaaggccg agggccgcgc
      781 tcgcacgttc gcggcgagca tccccgaaga cgagctgtcg aacctgatcg tgctgtgcga
      841 cccctgcgac gaggcgcgcc ggggctgttc gccggtggac ccggccttcc tcagcctgat
      901 cgtcgcgaag acgcgccgcg agcgatccgc gctcggccgg gccctcgacc cccgcaactc
      961 gcgccgggtc ctcgagtacc tgaggatgcg cgcgctctag agccgggccc cgaaccgctt
     1021 cagcacccgg gacgcgcgcg ggaacgactc ctccagggcc tcgaccaggt cgtcggtcgt
     1081 cgcgacctcg tacgcgttcc gggctacgta ccgccggagc gcgcagtcga ccttccgggc
     1141 cctgcccaag gcgtcgagcg ccttcacgcc ctggacgtag acgccgtcgt agtagtcgcc
     1201 ttcgtggccg tcccagaacc gcatgctctt gccgagccgt ccccgagcgg cttgcctcat
     1261 ctcgtagtcc cggaagtagc cgaggatgcc gtcgcctcgt gcctgggccc aggaggtgac
     1321 gccctcgtcg agccacgggt cccggccctg gttgttcccg acgagcccgt agaaccacga
     1381 gtgcgcgacc tcgtgggtcg tcgcccaggt caggctgtcg tctccctgga agatcatcgt
     1441 ggggtactcg atcccggagc ggcccagccc cggcacgatc gccatcgaga acgtgcgcca
     1501 ggggtacggt ccgtatcgct tcgagaggtc cctgagggcg cgggcgattg cgtcgcggaa
     1561 gtcgtccttg ctcgacccca gcccctcggc gaccccgacg gtgacctgta cggggttcgg
     1621 tgcgtcgacc ctcgcggtcg tgacgtcgaa gcgcccgacc gccacggcga agtcgcgcac
     1681 gtcctccgcc acccaccggc ccggcccgtt ctcgcgtccc gaggcgatga cgtcgaggcc
     1741 ctgcgggacc tcgatccgca cgtcgaagtc ggcggtcggg ctcgtcgacg cctcggcgag
     1801 cgtcgtcgtc ggcgggtcgg tcgcccaccc ccgctcgccc tcccacgcca ggatggggaa
     1861 gaaggagccg agccggatcg tgtcgccgtc ctgcgagatc cggtcgagga tcgggccggg
     1921 caggtcgagg gtccagtcca gctcggcgac gacggtctcg cccggcggaa gcgggtcgtc
     1981 cagctcgaag acgagcgtcg tcggatcggg ccggtcgacg gaccccccgc cgccgcgcag
     2041 ccgcgcgtcc tcgacggcga gctcggtgcc ctcggaggca agacgcgggc cgttgggcca
     2101 gagccggaac acgagcctgc tcgtcgggcg gtcggcgacg aacgtcacgt cggagtggcc
     2161 ggtgacggtc ccggcgtccg tgtcgacgcg catcgacatg tcgtagtggg ggacctcttc
     2221 gggaggagcc gcgcgcggag ggccgcactt gccggaccgc tcggagacgc ctccggtctt
     2281 ctcgggagcc ggagtggccg ccggggccgg cgtctcggtc gcgccggtcg cgtccggcgg
     2341 ggcggcgtcc gagcagccgg cgaggagcag gagagatgcg agcgcggccg cgatccgtcg
     2401 ccggttagta gcgtgtcgcg ccatgggaag catcgaggtc gtcaacttga cgcacagggt
     2461 gccggggggc cggacgctcc tcgaagaggc gacgttccgc gccggcgacg gcgaccacgt
     2521 cgcgctcgtc ggcgcgaacg gcaccggcaa gacgacgctg ctgcgactga taggtggtga
     2581 cgagaaggtc cagagcggcg cggtgcgcgt cgacggcgtg ctcgccgtca tgcgccagtt
     2641 cgtcgggatc cccggcgaga gcctcaccgt gcgcgacctc atgctccagc tctcgcccgc
     2701 gccgttgcag gccgctgcgc gcgctctcgc cgacgccgag gccgcgatcg cagaggacgg
     2761 cgggaagaag tcacagctcc ggtacgccgc ggccctcgcg gggtgggagt ccgccggcgg
     2821 ctacacggcc gaggtgctgt gggacacgtg cacgacgatc gccgtcaagc tcccgttcca
     2881 ccgcgtcgag gaccggccga tcgcgacgtt ctccggcggc gagcagaagc gcctcgcgct
     2941 cgaggtcatc ttccggtcgg acgcgacggt cgtgctgctc gacgagccgg acaacttcct
     3001 cgacctccag ggcaaggcat ggctcgagga gcagatgagg gcgtcgcaga agacgatcct
     3061 ctacgtgacg cacgaccgcg cgctcctcgc cgcgaccgcg caccggatcg tgacgctcga
     3121 agggaaccgc gtgtggacgc acggcggcgg cttcgagacc tatcacgccg cgcgcgagca
     3181 gcggatggat cgcatggagg agcagcaccg tcactaccgg caggagcacg agcggctgac
     3241 cgctctcgtg aaggagttca agaggaaggc cgcctacaac tcggactggg cgtcgcgcgc
     3301 cgaggccatg gagaagcgcc tgcggcgtta cgagaaggag aacgagccgc cgccgcggct
     3361 cgtcgagcag aacgtgcggg tgcgcgtggc cgggggccgg acgggcaaga tcgcgttccg
     3421 ggcgcgcggc ctcacgctgc cggggctcgt cgagccgttc gacaccgaga tccacttcgg
     3481 cgaacgcgtg gggatcctcg ggcgcaacgg caccggcaag agccacttcc tcaggctgct
     3541 cgcgggcgag gacgtgggcc acgaggggac gtggatgcta ggcgcccggg tcgagccggg
     3601 cttcttctcg cagacccacc agcaccccga atgggatccc aagccgatcc tcgacctcgt
     3661 gatggggacg ggcctgcagc ggaccccggc gatgtcggcg ctcaagcgct acgagctcgc
     3721 ggcggacgcg cagcaacctt tctcgaagct gtcgggcggg cagcaggcgc gtttgcagat
     3781 cctcatgctc gagatcgcga gcccgacgat gctgctgctg gacgagccga cggacaacct
     3841 cgacatcgcg tcggccgagg ctctcgagaa gggcctcgac ggctacaccg gcagcgtgct
     3901 cgcggtgacg cacgaccggt ggttcatgcg cgtgttcgac cgcttcctgg tgttccggga
     3961 ggacggcgtg gtcgaggagg ctctgacgcc cccgctcgag aaggccggct aaggcgcggt
     4021 catcatcacg acgccggcgg cggcgagcgc gaggcccccg acctgcagcc gccccatccg
     4081 ctcgcccagg aagatcctcg ccagcaggat cgtgctcgcg ggatacaggg acgtgagcac
     4141 cgccacgatc gacagcaggc cctctctcgt cgcgaggaga tagaggaggt tcgccgcgac
     4201 gtcgagcgct ccggcagcga cgatcgggac acgggtcccg ggggccgcgt ggatgcgctc
     4261 gcgccgcacc agcaccagga ccgtgaggat cgtgaacgac gccgtccggc ccgcgacgag
     4321 aggccacagc ccggtctcgt cgcccgcgcc gtccagcagg atgaagaacg ctccgaacgc
     4381 gacgcccgcg acgagggcgt ccgcgacccc cggagcgaac cggccccgag gcgggccgtc
     4441 cggctcgtcc tcggcgacgt cccgcggctc gaacccggct accagcacca ccgcggcgag
     4501 cgccacgacg acgcccacca gggcgagggc cgggggccgc tcgcccgtcg ccagccccca
     4561 gaccaccggg accacggcgg cttcgaccga agtgatcggc gccacgacgc tcatccgccc
     4621 cgccgccagc ccgcggtaga ggaagaggac ccccgtggac cccgcgacgc cgctggcgcc
     4681 tccccacgcg agggcggatg cggtcggtcc cgactcgacg aagaacggga cggcggcgaa
     4741 cagcagggcg gtcccggcta cctgcgacag cagtacgacc gtgaagaggg gggctcggcg
     4801 ggtgacgagc cccccgacga aatcgcccgc tccgtacgtc agggcggcgg ccagccccag
     4861 gaggatcgcc atgggcggca tgttcgcgcc attcaggaat gccctcgcac cgccgataaa
     4921 ccgctgacga gcatcagcgt tccagtggtg ggaggggcat ggcagtcaaa gccggtcgcc
     4981 tgttcggcgg ggcggcctcc gatgtcgtcc gggtgcgccc gggccggcgc gtcgcgggcg
     5041 cgatcgcgct cgtgctgccc atcgtcgtcg tcctgacggc gggcttcggg atcatcgcta
     5101 tccgccagcg tgccgacgat cgccgcgagg cgcaggtcat gctcagccgt atctcggctt
     5161 cggcgaaccg tcagagggtc ctcgagtcga acgcgctcga gctcgggctc gtcgccgagg
     5221 gcaggccgaa cgcgacgctc gctcgcgagc tcaggtccct gcccgacgag ctcgcccgcc
     5281 tcgacgacga ggtggcccag gcgctgacgc gactcagggc cgccgagccc ggctccaccg
     5341 agctggacga cctcgcggtc gcggtgaacc ggtaccaggt gtcgctcgaa gcgcagtccg
     5401 cactgctgac ggccggccgc ttcgacgagt cgttcggcta ccaccggtcg cgggtcgacc
     5461 tcgcgtaccg caacgtctcg caggagctgg cccagcagga tgcgcgcttc gacgccctca
     5521 cccaggacgc gagccggatg gccgacttcg ggacgctcgg catcctcggc tttgccctcg
     5581 tgctgctcgc gttcctccag ttcgaccgcg cgcgccgggc tgccgccgtc gacgaggcgc
     5641 agcgcaaggt cctgtcggag agcgaggcgc gcttccggtc gctggtccag aactcccgcg
     5701 acgtcatctg catcatcgac gagcgcgggc gcgcgctcta cgcgagccct gcggtcgccg
     5761 aggtgttcgg gatcacgccg gagcaagtgt gcggcgagga cctgctcctg ctcgcgcacc
     5821 cggacgagcg caagcgggtg cgcggctcgc tcgcggagct cgcggcggag ccgcgcggcg
     5881 cgtcgtcgaa gctcgagtgc cgcgtccgcc acgacgacgg ctcgttcaag gacctggagg
     5941 tcaccgcgac gaacctgctc gacgacgcct acgtcggcgg catcgtgctg aacgcgcgcg
     6001 acatctcgga gcgcaaggtc gccgagcgcg acaacgaagc cctgcaggag cagctcgcgc
     6061 accaggcctt ccacgacccg ctcaccgggc tggcgaaccg tgcgctcctg cgggagcgtc
     6121 tgcagcacgc gctcgctcgc gtcgcgcggt cgcgcgcgcc gctcgcggac atgttcatcg
     6181 acctcgacga gttcaaggcg gtcaacgact cgatgggcca cgacgtcggc gacgagctgc
     6241 tgaaggccgt cgccggacgg ctgggcgagt ccgtgcgtcc ggccgacacg atcgcgcggc
     6301 tcggcggcga cgagttcgcg atcctgctgg aggggatgtt cagcgcggcc gaggcgccgc
     6361 gcgtcgcgga gcgcatcatc cgcacgttca cggccccctt cgacctcggc gggcacccgg
     6421 tcacgatcgg ggccagcatc ggactcgcgc tgaaggacgg caaggagggg accgaggagc
     6481 tgctcggcaa cgccgacgtc gcgatgtacg cggcgaaggc gcgcgggcgc ggctgctatg
     6541 aggtctaccg cccggagctc aggctcgcgc gcacgcaacg gatccggcag gagctcgacc
     6601 tgcagacggc ggtcacgcgc aatgagttcg tcgtccacta ccagccgata gtcgacatgg
     6661 ctaccgggtg catgtcgggg gccgaggcgc tgctgcggtg ggcgcatccg acgaagggcc
     6721 tgatcccgcc gatggacttc gtggagctcg cggaggagac ggggctgatc gtgccgctcg
     6781 ggcggtggat cctccgggag gcgtgccgca acggcgcggc gtggaagaag acgcatccgg
     6841 aggccgcgga gatgaaggtc agcgtgaacc tctccgtccg ccagttccag gagacggacc
     6901 tcgtgcagca ggtcgccgac accctggagg tgacgcgctt ccctgcgtcc gacctcgttc
     6961 tcgagatcac cgagagcgtg ttcctcaacg actcgccggc gacgctcgcg gcgatccagg
     7021 gcctgaagga cctgggcgtg acgatcgcga tcgacgactt cggcaccggc tactcgtcgc
     7081 tcagctacct ccggtcgttc ccgatcgaca tgctgaagat cgaccggtcg ttcatcgacg
     7141 ggatcgaccg cggcgtcgag gacgcggccc tcgcccgcgc tatcgtgcag atcggcgaga
     7201 acctcaacct ccggatcgtg gcggagggga tcgagacgcc ggagcagctg cgcgaggtgc
     7261 ggcgcctcgg ctgccacgag ggccagggct tctttctggg gacccccctc gaccacgacg
     7321 agttgaaggc tctcctcgca cggggtgcga cgaggacgaa ctggctggac gacgctccgc
     7381 tggaggccgt gacctagatt ccagcgcgta gagtggagcc gtcatggcga tggtcccggt
     7441 cgacccagac ctcgacgagc tccgcagcac ggccgccacc tgcactgcgt gcgacctcta
     7501 tcgcaacgcg acccagaccg tcttcggcaa gggactcacg cgctcgcagg tgatgctcgt
     7561 cggcgagcag ccgggcgacc aggaggacgt cgcaggcgag cccttcgtcg ggccggcggg
     7621 ccgcctcctc gacgaggccc tcgtggccgc cggcatcgac cgcgaccagg cctacgtcac
     7681 gaacgtcgtg aagcacttca agttcgaaca acgcggcaag cgccgaatcc acaagaagcc
     7741 gaacatggcc gaaatcaccg cgtgccgtcc ctggctcgac gcggagatag aggtggtccg
     7801 gccggccgcg ctcgtgtgcc tcggcgcgac cgcggcccag gcgctgctcg gccgctcgtt
     7861 caaggtgacg cagcagcgcg gcgagttcgt cgagtccgac ctcgcgccgc tcgtgaccgc
     7921 gacgatccac ccgtcggcga tcctgcgcgc gcccgacgag cgcgaggtcg agatgaaggg
     7981 tctggtcgac gacctgacga aggtaaggaa agcgctcgcc aagctctagg gatgggacga
     8041 aagggggccc cgtgagcaag accgcccgct tcttcgccgg actgcgcgtc gccgacgaag
     8101 accccgcaga cgcgttcctg cgcgtgacca gctaccgggg ggcggaggtg atcgaggccc
     8161 ccgagggcac ggtcgaggtg ccggccaacc acgtgaggtt cagcgtctgg gtgggcgacc
     8221 gggcccactg cgtcgtgtcg atccccgagt cggaggcccg cgagctggcg gcctttctga
     8281 ccgccgagac gcaccgggtg gagacggtct gaagcaagac ggatacggag cgcgtccgct
     8341 gggtaaggac tcgcccatgc cggctcagga gaccgagcgc gtcgtcgggt cccggtacgc
     8401 cctcgaagag tccatcggac gcgggggcat gggcaccgtg tggcgcgcga ccgaccgcct
     8461 cctccagcgg gccgtggccg tcaaggaggt ccagctcccc gcgaccgttc ccgaggagga
     8521 gcgcgcggtg acgcgctcgc gggtactgcg cgaagcgagg gccgcggcga gcatctcgca
     8581 tcccggcgcg gtcatcgtct acgacgtcct cgaagaagag ggccgcccct acatcgtgat
     8641 ggagctcgtc gaggcaccga cgctcgaaga cgtggtccgc cgcgacggcc ccctcgacga
     8701 ggagcgcgcg gcccgggtgg ggctcgagat cctcggcgcg ctcgaagccg cgcacgcgca
     8761 ggggatcgtg caccgcgacg tcaagcccgc gaacgtcatg ctcccgcagg gccgccacgc
     8821 gaagctcgcc gacttcggca tcgcgagcgt caagggcgac ccgcggctga ccgcgacggg
     8881 actcgtgctc ggctccccgt cgtacatggc ccccgagcag gcgagcgaag acgtcaccgg
     8941 ccccgcctcc gacctgtggt cgctgggcgc gaccctctac tacgcggtgg agggcgagcc
     9001 gccgttcgac cgcggccagg cgatcccgac gctgacggcg gtggtggggg aggagccccg
     9061 cccgctccgc aaggcgcgag cgctgcgcga ggtcatcggg tcgctgctcg agaaggaccc
     9121 ggcgcagagg ccgtcgccgg aggtgttgag gcggatgctc gagcgggcgc tggcgggcgg
     9181 ttcgacgacg gaggtcgcgc ccccgccgac ggaggtcgtg accgagcccg ccgaggtcgc
     9241 gaccgaaccc gcgcctgtcg aacccgcgcc ggtccctgcg gccccgcgtg caaggccgtg
     9301 gctcgccatc ctcgccgcgg ccgggctcct gctcgtcgtc gccgtcttgc tgttcctctc
     9361 gatgggcgag gacgagggtg gagccggaaa cgaagccggc cggaaccgag gcgcggcgaa
     9421 cgagcgcgga gacgagacgg accccgagga gcccgcggag gaggccgaac cggcgggcgt
     9481 cgtgccggag ctcaccgagt acgaggatcc cgaggccggc taccaggtct cgtacccgga
     9541 gggctgggac gtcgagccgc tcggcgacac ccggacggac ttcaccgatc ccgagaccgg
     9601 cgcgtacctg cgtatggact ggacgcccac gccgggcgac gaccccgtgg cggcgtggga
     9661 gggacagtcg gcgagcttcg ccggccggta cgcggactac gaggagatcc gcgtcgaccc
     9721 ggtgacgttc cagggcttcc cggcggccga gtgggagtac acgtactccg gcgtgcacgc
     9781 gatcaacgta ggggtcgtca ccgacacgta cggctacgcg ctgaacttcc agacccgagc
     9841 cgaggactgg gaggagtcgc aggccctgtt cgaggcgatc aaggagacgt tcgtccccgc
     9901 cccctagcgc ccgtctaccg gatcggggga gccgtttccc cttgtagccg catagttcct
     9961 gctgttacat agcgcttcac accaccgaga cttggggggc aaagtggggc gtatcgccag
    10021 tgctcgcatt cacccggcca tcggtattgc gagggtgggt gacagcccgg acgagtactt
    10081 cgtcggcccc gaggtcctgc gcccgaggca agaggacgcc tacaaggacg cgcagggccg
    10141 gttgaagcgc caggctgcgc gcttccgcat ctacggctac gactccgacg gcgagctcgt
    10201 cggcgagatc acgtcggccg acgcggagat cgtgtgggag gtccacgtcg cgaacacgaa
    10261 gggggcctgg tacgacttcg tcgtcgcgct cgacctcacg gaagccgcag ccgtgaagtc
    10321 gcctcggcgc aactcgtgga tggggcgcga gggccgcgac gagctcgtg
//
LOCUS       DASQDO010000061         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1108442, whole genome shotgun sequence.
ACCESSION   DASQDO010000061 DASQDO010000000
VERSION     DASQDO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108442"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VEV43_02000"
     CDS             complement(<1..1107)
                     /locus_tag="VEV43_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYP22321.1"
                     /translation="MGGIGGHARATPDAPALVTLARRLTFGELDERQQRLAGLLHAGG
                     LRARDRIAVWSSNRLESLEVTTGALRAGIVPVPVNALLTPSEAAYLIEDSGARWLFTD
                     RPVEPWPPLERSVTFGDAYERCLHEAEPASISDYALGRPRHYTSGTTGAPKGVWVEPA
                     GEAAAGETSRDFASQWGLTPDEVHLLCSPLAHSAPHRFSLRTLEAGGSVVLQARFEAM
                     ETLAAVELFGVTSTFVVPTHLERIFSLEPKVLKRHYSSSMRMLIHAGAPIREATKRRA
                     IEFFPEGSVWEFYGSTEGGATRISPEEWLRKPGSVGTARPGAAILIRDETGAPVPRGE
                     VGVVWVRDPHAERFSYWGDEEKTRGAWDGDAFTVG"
     gene            complement(1115..1675)
                     /locus_tag="VEV43_02005"
     CDS             complement(1115..1675)
                     /locus_tag="VEV43_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22322.1"
                     /translation="MEDLLARRRWPFLAFAAVGVVACVVIVALPDSLLAQMERNRPLD
                     SGQAGWAYRLLVLFALVAAAFTMFRAFTVDSVKRSQERDPKTRAMPAERVVDSVSQNA
                     ASVPLLTVVYGVASVIFTGQRGGFWLFPLIALGQGAWHYRSVGEIARWLSFQPEPEVE
                     EEVSTEWKREPPDYCPPLARGLLTGR"
     gene            1753..2316
                     /locus_tag="VEV43_02010"
     CDS             1753..2316
                     /locus_tag="VEV43_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP22323.1"
                     /translation="MEVRPDVQLIERLLVRDESALREVVDEYGGVVHGMARRVLADPT
                     LAEEVAQDAFLALWRRPGAFDPARGSLQSFLLGIVRNKAVDLVRKEQSLRRTRDVLAK
                     ELPVTEQTHDPGAEVEERQRVSAALGKLSEVQREAIVLAYFGGRTYREVAHELEIPEG
                     TAKTRLRDGLIRLREALAPTQESEGWS"
BASE COUNT          380 a          885 c          818 g          320 t
ORIGIN      
        1 gccgaccgtg aacgcgtcgc cgtcccacgc gccccgggtc ttctcctcgt ccccccagta
       61 cgagaaccgc tcggcatggg ggtcgcgcac ccagaccacg ccgacctcgc cccgcggtac
      121 gggcgcaccc gtctcgtccc ggatcaggat cgccgcgccc ggccgggccg tcccgacact
      181 gcccggcttc cgcagccact cctccggcga gatccgggtc gcgcccccct cggtcgaccc
      241 gtagaactcc cacaccgagc cctccgggaa gaactcgata gcgcgccgct tcgtcgcctc
      301 gcggatcggg gcccccgcgt ggatgagcat ccgcatgctc gacgagtagt ggcgcttcag
      361 caccttcggc tcgagcgaga agatccgctc gaggtgcgtc ggcacgacga acgtcgaggt
      421 gacgccgaag agctcgaccg cggccagggt ctccatcgct tcgaagcgcg cctgcagcac
      481 gacgctcccg cccgcttcga gtgtccgcag cgagaaccga tgcggcgcgg agtgcgcgag
      541 cggcgagcac aagaggtgta cctcgtcggg cgtgagcccc cattgcgacg cgaagtcgcg
      601 cgaggtctca cccgcggcgg cctcgccggc cggttcgacc cacaccccct tcggcgcccc
      661 cgtcgttccc gaggtgtagt gcctcggccg cccgagtgcg tagtcggaga tcgaagccgg
      721 ctctgcctcg tgaagacacc gttcgtacgc gtctccgaag gtcacggatc tctcgagcgg
      781 aggccacggc tcgaccggcc ggtcggtgaa cagccagcgc gcgccggagt cctcgatcag
      841 gtacgcggct tcggagggag tcagcaacgc gttgaccggc accggcacga tcccggcccg
      901 cagcgcgcct gtggttacct cgagcgactc caggcggttc gacgaccaca ccgctatccg
      961 gtcgcgggcg cgtagcccac cggcatgcaa gagcccggcg agccgctgct ggcgctcgtc
     1021 gagctcgccg aacgtcagcc ggcgagcgag agtgaccagc gcgggcgcgt cgggcgtcgc
     1081 acgcgcgtgg cccccgatcc ctcccacgag gtcttcagcg cccagtaagc agcccgcgcg
     1141 ccagcggggg gcagtagtcc ggaggctccc gcttccattc cgtcgagacc tcctcttcca
     1201 cctcgggctc cggctggaac gagagccacc gcgcgatctc gccgaccgag cggtagtgcc
     1261 aggccccctg cccgagggcg atcagcggga agagccagaa gccccccctc tgcccggtga
     1321 agatgaccga agccacgccg tagacgaccg tcaacagcgg gacggaggcg gcgttctgcg
     1381 acaccgagtc gacgactcgc tccgccggca tggccctcgt cttggggtcg cgctcctgcg
     1441 agcgcttgac cgagtcgacc gtgaacgccc ggaacatcgt gaaggccgcc gcgacgaggg
     1501 cgaagaggac caggagccgg taggcccacc ccgcctgtcc gctgtccagc gggcggttgc
     1561 gctccatctg ggcgagcagg ctgtcgggca gggcgacgat gacgacgcac gccacgacgc
     1621 cgaccgcggc gaaggcgaga aacggccacc ggcggcgtgc gaggaggtct tccacgtggc
     1681 gagcgtaacg gccaatccac gacgcctagg tcttccgaac cctctccgac agcacgccac
     1741 tacgatgccc cgatggaggt ccgtcccgac gtgcagttga tcgagaggct cctcgtccgg
     1801 gacgagtctg ccctccgcga ggtcgtcgac gagtacggag gtgtcgttca tgggatggcc
     1861 aggcgcgtcc tcgccgaccc cacgctcgcc gaggaggtcg cacaggacgc gttccttgcg
     1921 ctgtggcgcc ggccgggcgc gttcgacccg gcccgcggca gcctccagtc gttcctgctc
     1981 ggcatcgtcc gcaacaaggc cgtcgacctc gtccgcaagg agcagtccct gcgccggacc
     2041 cgcgacgtcc tcgccaagga gctaccggtg accgagcaga cgcacgaccc gggcgccgag
     2101 gtcgaggagc ggcagcgcgt gtcggccgcg ctcggcaagc tcagcgaggt ccaacgagag
     2161 gcgatcgtgc tcgcgtattt cgggggccgg acgtaccgcg aggtcgctca cgagctcgag
     2221 atccccgaag gcacggcgaa gacccggctg cgcgacgggt tgatccgctt gcgcgaggca
     2281 ctggccccga cacaggagag cgagggatgg tcgtgacgca cgacgagatc aagtccctca
     2341 tcgcgcccta cgtgctcggt gcggtgacgg ccgaggagga gcgcgctgtc cgctcgcaca
     2401 tca
//
LOCUS       DASQDO010000062         6732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_130000, whole genome shotgun sequence.
ACCESSION   DASQDO010000062 DASQDO010000000
VERSION     DASQDO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130000"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VEV43_02015"
     CDS             <1..1512
                     /locus_tag="VEV43_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP22324.1"
                     /translation="SRALAGSPELDEAIRGVLDAVREIVCARETLVVLKLGRQTVWSA
                     VDTDGVVAQMAPLEIGPLASLMMEVEMREAALSLSQDGGDAERAILDALGVRTLAAVP
                     LLDEEGVLGCLVAVDRIGAGDFGNGDALLLEAVAAELLTTLGSHRLYAEVAEERQRFA
                     RIFHGSREGIALLDHRGYVRAWNPALERITGYRAADVMGRPLADRVVLRRDDQRRVAT
                     EEITAASETNEVEVVTKEGPGRWVSVTAGPVDVGEHVAHVLLVRDVTAEHEAEEAKSD
                     FLSTISHELRTPLTSIKGALQVLARGSQNLSDDLSDQMVTVMQRGSDRLERLVMNLLL
                     VSQIESGGLAHSSDGTVTLDELVGDTASRVLGDHRHRVVVEEPDVVVRADADRLEQAI
                     GHVLDNAAKFGGPDGLVTITVGRDHGYARVSVTDEGPGIARSDHDRIFDRFVRLGDVL
                     TRETQGAGVGLFIARRALSSMGGDVWVESEPGRGATFHLNVPLARPVAVAHTA"
     gene            1523..2836
                     /locus_tag="VEV43_02020"
     CDS             1523..2836
                     /locus_tag="VEV43_02020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HYP22325.1"
                     /translation="MKIGVIGTGHVGLVTCASLAKVGHEVVGVDVDAEKIAMLRDGRS
                     WFYEPGLDEMLAELLDGGSLSFSTQSEDAVAEADVAFICVGTPPRATGEANLVAVEQS
                     AIDVARKSTKQTVVVEKSTVPAGTAQRLRRTLKQVRSSSLPELEIASNPEFLREGKAI
                     EDSMHPERILVGADSEWAFEKMREVYAPWVAQGYPLVETNIATAELAKHACNAFLSLK
                     ISFANALARMCERAGADVVDVVDVMGSDSRIGRAFLNAGLGYGGYCFPKDIQAFERLA
                     SGLGYDFPLLREVERINEEALDAAVEKVVDALWNLEDKRVALFGLAFKPETDDSRFAP
                     ALGVARRLLDRGADVVAYDPVVRDEAAAEVPGLRLAPDLYAAADGADCVVVCTEWQEF
                     KDVDLAKLRSVMSRPIIVDGRNVFDPESVGAAGFAYYPMGRRAIV"
     gene            2844..3779
                     /locus_tag="VEV43_02025"
     CDS             2844..3779
                     /locus_tag="VEV43_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HYP22326.1"
                     /translation="MPTALVTGGAGFLGSHLCERLVREGWDVVCHDSLLTGDAANLDH
                     LGSEPRFRFEERDVTVSVDVEGPVDWVLHFASPASPRDYLELPIETLQVGSLGRLNAL
                     ELARDKGAGFMVASTSEVYGDPKEHPQNESYWGHVNPIGPRSVYDEAKRFTEAATMAF
                     HRTHGVPVRIIRIFNTYGPRMRRNDGRAVPNFVEQALNGRPITIYGDGSQTRSLCYVD
                     DLIEGIYRFLRSDERGPMNIGNPHEVSILELARMINEAAGGKSELVFEERPVDDPEMR
                     RPDIGYARSALGWEPVVPLEEGLARTMEWAAAAWA"
     gene            complement(3843..4214)
                     /locus_tag="VEV43_02030"
     CDS             complement(3843..4214)
                     /locus_tag="VEV43_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22327.1"
                     /translation="MRAEQNPPPVELVTESVAPGWILVSPSALPDRWRDRATPMFLVP
                     LLPGEAHDVLSGEPAGSELHGEDLALARLVARGVPVEDCARQLHLTSRTVYRRLARLR
                     RLVGVKTTSELVAELARRGFR"
     gene            4485..5630
                     /locus_tag="VEV43_02035"
     CDS             4485..5630
                     /locus_tag="VEV43_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP22328.1"
                     /translation="MTTQVESSFEAVWPDLERRIHRFLSSKKVPDSQREDVVQETGLR
                     LYRMWAQVDPAGSPIGLALTIAMNIVRDNARRDSHRTVVEIEPDVPAICDVERSGLAR
                     LELSRVRRALAELTPAQRSVLLSELDRNQRPPEASPAAIKMLRMRARRNLSAILERAG
                     AGVLVGKVRRFFGLPQHVIVSRDLPKVEQGVASSLASVMAAAAISLSPITGLLGFGGA
                     DAAEDPAPGPHAVAEEVWLAGSSVARATATLPQEAIAGDPRPANLLGESPLVRRSEAA
                     VKAKGAGRAAKAPRDEEAPVVDVALPNGEGQIQGNGSVEVHVPPPTLKPHDDNPFCDP
                     TQPGCTPDPSLPATGEAEAYIRVREVEVEVEVNTDEVADTLEAAIGV"
     gene            5729..6232
                     /locus_tag="VEV43_02040"
     CDS             5729..6232
                     /locus_tag="VEV43_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22329.1"
                     /translation="MAQSPDGSIRVTVAGREFGLTRAMVEERLADVLPEPLTDHFVVV
                     GGRRFPPKQVVALATGLDRADFTTHHARRVLRRLGFPAGRRSDPSDSPSEPARVGSHG
                     GAQAEALRPFIGKWVAVKGIEVLVAADTPEEVVSWVERHDVYADGMFRVPEDPDFDVR
                     AILGIEI"
     gene            6279..6620
                     /locus_tag="VEV43_02045"
     CDS             6279..6620
                     /locus_tag="VEV43_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22330.1"
                     /translation="MPVVVEGHERVPHFSIVDSGSLHNRFDRWIARMWGIDLTGATDE
                     IIGLGGFSTVARTVTTRLSIGGFAWEAPVSFCEPWPLDFQLRGQGGCFRWFEVTIRAA
                     DLSIDLEPEVS"
     gene            6631..6703
                     /locus_tag="VEV43_02050"
     tRNA            6631..6703
                     /locus_tag="VEV43_02050"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6664..6666,aa:Thr,seq:ggt)
BASE COUNT         1025 a         2317 c         2397 g          993 t
ORIGIN      
        1 agccgcgctc ttgccggcag ccccgagctc gacgaggcga taaggggggt cctcgacgcc
       61 gtgcgcgaga tcgtgtgcgc gcgcgagacg ctcgtcgtgc tgaagctcgg acgtcagacg
      121 gtgtggtcgg cggtcgacac cgacggcgtc gtcgcgcaga tggcgccgct cgagatcggt
      181 cccctggcct ctctcatgat ggaggtcgag atgcgcgagg cggcgctgtc gctctcgcag
      241 gacggcggcg acgccgagcg cgcgatcctc gacgcgctgg gcgtgcgcac gctggcggcg
      301 gtgcctttgc tcgacgagga aggcgttctc ggctgtctgg tcgcggtcga ccgcatcggc
      361 gccggtgact tcggcaacgg tgacgcgctg ctgctcgagg cggtagcggc cgagctcctg
      421 accacgctcg gttcgcaccg gctctacgcg gaggtcgcgg aggagcgcca gcggttcgcg
      481 cgcatcttcc acgggtcacg cgagggcatc gcgctcctgg accaccgcgg ctacgtcaga
      541 gcgtggaacc cggcgctcga gcggatcacc ggctaccgcg ccgccgacgt catgggccgt
      601 cccctcgccg accgcgtggt cctccgccgc gacgaccagc gccgcgtcgc gaccgaggag
      661 atcaccgccg cgagcgagac gaacgaggtc gaggtcgtca ccaaggaggg cccgggacgc
      721 tgggtctcgg tcaccgccgg gcccgtcgac gtaggcgagc acgtcgcgca cgtgctgctc
      781 gtccgcgacg tcaccgcgga gcacgaggcc gaggaggcga agtcggactt cctgtcgacg
      841 atcagccacg agctgcgcac tccgctgacg agcatcaagg gcgcgctcca ggtcctcgca
      901 cgcggctccc aaaacctgtc cgacgacctc tcggaccaga tggtcacggt gatgcagcgg
      961 ggctcggaca ggctcgaacg gctggtgatg aacctgctgc tggtctcgca gatagagagc
     1021 ggtggcctcg cgcactccag cgacgggacg gtcacgctcg acgagctcgt cggcgacacg
     1081 gcgtccaggg tgctcggcga ccaccggcat cgcgtggtgg tcgaggagcc cgacgtggtc
     1141 gtgcgcgccg atgccgaccg cctcgagcag gcgatcggcc acgtgctcga caacgccgcg
     1201 aagttcggcg gccccgacgg cctcgtgacg atcaccgtcg ggcgcgacca cggctacgcc
     1261 cgcgtgtcgg tgaccgacga agggcccggc atcgcccgct ccgaccacga ccggatcttc
     1321 gaccgcttcg tccggctggg agacgtgctc acgcgtgaga cgcagggggc cggggtgggt
     1381 ttgttcatag ctcggcgtgc gctctcgtcc atgggcggcg acgtctgggt cgagagcgag
     1441 ccgggccgcg gcgccacctt ccacctgaac gtccccctcg cgcggcccgt ggcggtggcc
     1501 cacacggctt agtcttcccc gcatgaagat cggggtcatc ggaacgggac acgtcgggct
     1561 ggtcacctgc gcctcgctcg cgaaggtcgg ccacgaggtc gtcggggtcg acgtcgacgc
     1621 ggagaagatc gcgatgctgc gggacggccg gtcgtggttc tacgagccgg ggctggacga
     1681 gatgctggcc gagctcctcg acggcgggtc gctgagcttc tcgacccagt cggaggacgc
     1741 ggtggccgag gctgacgtcg ccttcatctg tgtggggacg ccgccccgcg cgacgggcga
     1801 ggcgaacctc gtcgccgtcg agcagtcggc gatcgacgtc gcgcggaagt cgacgaagca
     1861 gacggtcgtc gtcgagaagt ccactgtccc cgccggcacg gcccagcgcc tgcgccgcac
     1921 gctcaagcag gtccggtcgt cgtcactgcc cgagctcgag atcgcctcga accccgagtt
     1981 cctgcgcgag ggcaaggcga tcgaggactc gatgcatccc gagcggatcc tcgtcggggc
     2041 cgactccgag tgggccttcg agaagatgcg cgaggtctac gcgccgtggg tcgcgcaggg
     2101 ctaccccctc gtcgagacca acatcgcgac cgcggagctc gcgaagcacg cgtgcaacgc
     2161 gttcctgtcg ctgaagatct cgttcgccaa cgcgctcgcc aggatgtgcg aacgggccgg
     2221 agccgacgtc gtcgacgtcg tcgacgtgat gggctcggac tcccgcatag ggcgcgcgtt
     2281 cctcaacgcg gggctcggct acgggggcta ctgcttcccg aaggacatcc aggcgttcga
     2341 gcgcctggcg agcgggctcg gctacgactt cccgctgctg cgagaggtcg agcgcatcaa
     2401 cgaagaggcc ctcgacgcgg ccgtcgagaa ggtcgtcgac gcgctgtgga acctcgagga
     2461 caagcgcgtc gcgctgttcg gcctcgcgtt caagcccgag accgacgaca gccgcttcgc
     2521 cccggccctc ggcgtcgcgc ggcggctgct cgatcgcggc gcggacgtcg tcgcgtacga
     2581 cccggtcgtg cgggacgagg cggccgcgga ggtgcccggg ctgcggctgg cccccgacct
     2641 ctacgccgcc gccgacgggg ccgactgcgt cgtcgtctgc acggaatggc aggagttcaa
     2701 ggacgtcgac ctcgccaagc tgaggtcggt gatgtctcgg ccgatcatcg tcgacggccg
     2761 caacgtcttc gatccggagt cggtcggcgc cgcgggtttc gcgtattacc cgatgggccg
     2821 ccgggccatc gtctgagccg cccatgccga cagccctcgt aaccggcgga gccgggttcc
     2881 tcggctcgca cctctgcgag cgactcgtgc gcgagggatg ggacgtcgtc tgccacgact
     2941 ccctgctgac cggcgacgcc gccaacctgg accacctcgg ctccgagccg cgcttccggt
     3001 tcgaggagag agacgtcacg gtctcggtcg acgtcgaagg gccggtcgac tgggtgctgc
     3061 acttcgcctc gccggcttct ccccgcgact acctcgagct gccgatcgag acgttgcagg
     3121 tcggctcgct cgggaggttg aacgccctcg agctggcgcg ggacaagggg gccggcttca
     3181 tggtcgcgtc gacgtccgag gtctacggcg acccgaagga gcatccgcag aacgagtcgt
     3241 actggggtca cgtcaacccg atcgggccgc gctccgtgta cgacgaggcg aagcgcttca
     3301 ccgaggccgc gacgatggcg ttccaccgca cgcacggcgt gcccgttcgg atcatccgga
     3361 tcttcaacac ctacggcccc cgcatgcgcc gcaacgacgg acgagcggtg ccgaacttcg
     3421 tcgagcaggc cttgaacggg cgtccgatca cgatctacgg cgacggcagc cagacgcgca
     3481 gcctctgcta cgtcgacgac ttgatcgagg gcatctaccg gttcctgcgg tcggacgagc
     3541 gcggcccgat gaacatcggc aacccgcacg aggtctcgat cctcgagctc gcccggatga
     3601 tcaacgaagc cgcgggcggg aagtccgagc tcgtgttcga ggagaggccc gtcgacgacc
     3661 cggagatgcg gcggcccgac atcggctacg cccggagcgc gctcggctgg gagccggtcg
     3721 tcccgctcga ggaaggcctc gcccggacga tggagtgggc ggccgcagcc tgggcttaac
     3781 gacgggtcgc ctaggcgagc cgccaagaac gacgggtcgc ctcggcgagc cgccgcaagc
     3841 ctttagcgga agccgcggcg ggcgagctcg gcgacaagct cgctcgtcgt cttcacgccg
     3901 acgagccggc ggagccgggc cagcctcctg tacacggtcc gggacgtcag gtgcagctgg
     3961 cgggcgcagt cctcgaccgg cacgccccgg gcgacgaggc gggcgagcgc caagtcctcg
     4021 ccgtggagct ccgaccctgc gggctcgccg gacagcacgt cgtgggcctc gcccggcaag
     4081 agcgggacga ggaacatcgg ggtggcccga tcccgccacc ggtcgggcaa cgccgacggc
     4141 gacaccagga tccagccggg ggctacggac tccgtgacca gctcaacggg tggggggttc
     4201 tgttcggctc tcacgcagtt tcctttgcct cgggatgcgc ttgcagctca tatgggggat
     4261 acgtgcggag ggccgagaga taacgcccgg cgtcacgcga gctgacagcc cccgtcaccg
     4321 agacgacgaa atccggtccc gtgcaggtcc cgaagcagtc ccgcgtccaa tttctgttac
     4381 tagtgcccgg ttcgtgcgta ttaagcagag caatggcatg atcgggtcgc tttctcagcg
     4441 ggtcgtccat gcggtgtggg cggagaaatc cgggggagat gaagatgacg acgcaggtgg
     4501 agtcgagctt cgaagcggta tggccggacc tggaacggcg cattcaccgg ttcctcagca
     4561 gcaagaaggt tcccgacagc cagcgtgagg acgtggtcca ggagaccggc ctccgcctct
     4621 atcggatgtg ggcccaggtc gacccggccg gctcgccgat cgggctggcc ctgacgatcg
     4681 cgatgaacat cgtccgggac aacgcccgcc gcgactccca ccgcacggtg gtcgagatcg
     4741 agccggacgt ccccgcgatc tgtgacgtcg agcgctccgg gctggctcgc ctcgagctca
     4801 gccgcgtcag gcgtgctctc gccgagctga cccccgccca gcggtcggtg ctgctctccg
     4861 agctcgaccg caaccagcgc ccgcccgagg ccagccccgc ggcgatcaag atgctgcgga
     4921 tgagggcccg gcggaacctc agcgcgatcc tcgagcgcgc gggggccggt gtcctcgtcg
     4981 gcaaggtccg gcgcttcttc ggcctgcccc agcacgtcat cgtctcgcgt gacctcccga
     5041 aggtcgagca gggcgtggcc tcgagcctcg ccagcgtgat ggctgcggct gcgatctcgc
     5101 tgtcgccgat cacggggctg ctcgggttcg gcggtgccga tgcggccgaa gacccggccc
     5161 ccggcccgca cgcggtggcc gaagaggtat ggctggcggg ttcgtccgtc gcccgggcga
     5221 cggcgacgct gccgcaggag gccatcgccg gcgacccgag gcccgcgaac ctcctcggcg
     5281 agtccccgct cgtgcggcgt tccgaggccg cagtcaaggc gaagggcgcc ggccgggcag
     5341 cgaaggcccc gcgggacgaa gaggctccgg tcgtcgacgt cgcgctgccg aacggcgagg
     5401 gccagatcca gggcaacggg tccgtcgagg tccacgtccc gccccccacg ctgaagcccc
     5461 acgacgacaa cccgttctgc gacccgaccc agccgggctg cacgccggac ccgagcctgc
     5521 cggcgacggg cgaggccgag gcgtacatcc gggtccgcga ggtcgaggtc gaggtcgagg
     5581 tgaacacgga cgaggtggcc gacacgctgg aagctgcgat cggcgtctag atccatccgg
     5641 tttggaagag gcggccccct cgggggccgc tttttcgttg gccgacccac ccttgacaga
     5701 gacgaatcag agaaaccaaa atggagacat ggcgcagagc cccgatggtt ccatccgcgt
     5761 gaccgttgcc ggacgggagt tcgggctgac gcgcgcgatg gtcgaggagc gcctcgccga
     5821 cgtcctgccc gagcccctca ccgaccattt cgtcgtcgtc ggcggacgcc gctttccccc
     5881 gaagcaggtc gtagcgctgg cgaccgggct cgaccgcgca gacttcacga cccaccacgc
     5941 ccggcgggtc ctgcgccgcc tcgggttccc cgcggggcgg agatcggatc cctccgactc
     6001 gccgagtgag cctgcgcggg tgggatccca cggaggcgcc caggccgagg ctcttaggcc
     6061 gttcatcggg aagtgggtgg ccgtgaaagg gatcgaggtc ttggtggcgg ccgacacacc
     6121 cgaggaggtc gtctcctggg tagaaaggca cgacgtgtac gccgacggca tgttccgcgt
     6181 ccccgaggac ccggacttcg acgtgagggc gatcctcggc attgagattt gacttcttgg
     6241 agctcccatc ttcgagcggc gggcgtcctc ggccgattgt gccggtcgta gtggagggcc
     6301 acgagcgcgt cccgcacttc agcatcgtcg actccggatc gcttcacaac aggttcgatc
     6361 ggtggatcgc acgcatgtgg gggatcgacc tgacgggcgc aaccgacgag atcatcggcc
     6421 tcggcggttt cagcacggtg gcaaggaccg tcaccacccg gctctcgatc ggcggcttcg
     6481 cctgggaggc ccccgtctcg ttctgcgagc cctggcccct cgacttccag ctccgcggcc
     6541 agggaggatg cttccgctgg ttcgaggtca cgatccgggc agccgacctc tcgatcgacc
     6601 tcgagcccga ggtatcctga accggctcag gccccgttag ctcagacggc agagcgtctc
     6661 catggtaagg agaaggtcca cggttcgatt ccgtgacggg gctcgctcgc ggtgccacga
     6721 tcggcagcaa ac
//
LOCUS       DASQDO010000063         1940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_925441, whole genome shotgun sequence.
ACCESSION   DASQDO010000063 DASQDO010000000
VERSION     DASQDO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925441"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VEV43_02055"
     CDS             complement(<1..839)
                     /locus_tag="VEV43_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP22331.1"
                     /translation="MPANEVLLEVKDLHVHFPTSDGLVKAVDGLNFTVRRGETFGIVG
                     ESGSGKSVTCLTALGLTNLDKAEVSGEVLFKGQNLLALDKEELRGLRGKEMAMIFQDP
                     FACLHPFYRVGDQIVEAILVHEKMPKNRAAERAVELLAAVGIPKPRDRFKGYPHEYSG
                     GMRQRAMIAMALAHNPDLLIADEPTTALDVTVQAQILELIDNVKREFDIGVILITHDL
                     GVIADVAQTVMVMYAGRSMERGSKEDLFGRPLHPYTWGLLESMPRVSQKGTRLLPIEG
                     SPPS"
     gene            complement(875..1861)
                     /locus_tag="VEV43_02060"
     CDS             complement(875..1861)
                     /locus_tag="VEV43_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP22332.1"
                     /translation="MAQYVVRRMMWSIVMLVLVIASVFVIFFVMPGGAGGRTADGQSL
                     LAIKFAGKNPRAEVVDQVEKRLGLDQPVVVQFGRYVGNAFTGDLGYSYQTQEDVLDAL
                     LGRFPATAGLALGASVLWLAIGVSIGIISALKRRSLLDRGSMLFALIGVSMPTFWLGL
                     IAVFTFDAGPIQALQVYDTGSYVPFTENPWEWFKVMWLPWFVLAFTSAAIYARMVRGN
                     LLEVQGEDYIRTARAKGLREKSVTRHALRSALTPIVTMYGLDLGILLGGAVITEQIFN
                     IPGIGSYAVGAIRSQNFPIILGTTVFAAGFVIIANLVVDILYAALDPRVRYS"
BASE COUNT          325 a          643 c          664 g          308 t
ORIGIN      
        1 gatggcggcg acccctcgat cggaaggagc cgcgttccct tctgcgacac gcgcggcatc
       61 gactcgagga ggccccacgt gtacgggtgg agcggccggc cgaagaggtc ctccttggag
      121 ccgcgctcca tcgagcggcc cgcatacatg accatgaccg tctgcgccac gtcggcgatc
      181 acgccgaggt cgtgggtgat caggatcacg ccgatgtcga actcgcgctt cacgttgtcg
      241 atgagctcga gtatctgggc ctggaccgtc acgtcgaggg ccgtggtggg ctcgtcggcg
      301 atgagcaggt cggggttgtg cgcgagcgcc atcgcgatca tcgcgcgctg cctcatcccg
      361 cccgagtact cgtgcgggta gcccttgaag cgatcgcgcg gcttggggat gcccaccgcc
      421 gcgaggagct cgaccgcgcg ttccgcggcc cggttcttcg gcatcttctc gtgcacgagg
      481 atcgcctcga cgatctggtc gccgactcgg tagaaggggt ggaggcaggc gaacggatcc
      541 tggaagatca tcgccatctc cttgccgcgc aggccgcgca gctcctcctt gtcgagcgcc
      601 aggaggttct ggcccttgaa gaggacctcg ccggagacct cggccttgtc gaggttcgtg
      661 agaccgagcg cggtcagaca cgtcacgctc ttcccggaac cggactcgcc gacgatgccg
      721 aaggtctcgc cgcggcggac cgtgaagttc aggccgtcca cggccttcac cagaccgtcg
      781 ctcgtcggga agtggacgtg caggtccttg acctcgagga ggacctcgtt cgccggcatc
      841 ggagaagtgt agagcctgac gtgcccgttc gggcctagga gtaccggacc cgcgggtcga
      901 gggccgcgta caggatgtcg acgaccaggt tggcgatgat cacgaacccg gccgcgaaca
      961 ccgtcgtccc caggatgatc gggaagttct ggctcctgat cgcaccgacc gcgtagctgc
     1021 cgatcccggg gatgttgaag atctgctcgg tgatgaccgc gcctccgagc aggattccga
     1081 ggtcgagccc gtacatcgtc acgatcggcg tgagcgcgct gcgcagcgcg tggcgcgtca
     1141 ccgacttctc gcgcaggccc ttcgcgcgcg cggtccggat gtagtcctcg ccctggacct
     1201 cgaggaggtt gccgcggacc atgcgggcgt agatcgcggc cgacgtgaac gccagcacga
     1261 accacgggag ccacatgacc ttgaaccact cccacgggtt ctcggtgaag gggacgtagg
     1321 acccggtgtc gtagacctgc agcgcttgga tcgggcccgc gtcgaaggtg aacaccgcga
     1381 tgaggccgag ccagaacgta ggcatcgaca ccccgatgag cgcgaacagc atcgacccgc
     1441 gatcgagcag cgatcttcgt ttgagcgcgg agatgatgcc gatggacacg ccgatagcga
     1501 gccacaggac cgaagcgccg agggcgaggc cggcggtcgc ggggaagcgg ccgaggagcg
     1561 cgtcgaggac gtcttcctgc gtctggtacg agtagccgag gtcgcccgtg aacgcgttgc
     1621 cgacgtagcg cccgaactgg acgacgacgg gctggtcgag gccgaggcgt ttctcgacct
     1681 gatcgacgac ctcggcccgc gggttcttcc ccgcgaactt gatcgccagg aggctctggc
     1741 cgtcggccgt ccggcccccg gccccgcccg gcatgacgaa gaagatcacg aacacggagg
     1801 cgatcacgag gaccagcatc acgatcgacc acatcatgcg tctcacgacg tactgggcca
     1861 tgcgaccttt ccctccgtct ttcgttcctc gcccgatacg acgagcggtg cgggggccgc
     1921 gcggcccccg caccacccgc
//
LOCUS       DASQDO010000064         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_435788, whole genome shotgun sequence.
ACCESSION   DASQDO010000064 DASQDO010000000
VERSION     DASQDO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435788"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VEV43_02065"
     CDS             complement(<1..409)
                     /locus_tag="VEV43_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HYP22333.1"
                     /translation="MLTDVIDDLACVVCGGAVSAAGGSVRCEAGHSFDVARQGYVTLT
                     AGAGDSAEMVAAREAFLGAGHYRPITEAVARHVDPAGPVVDLGAGTGHHLAGVLAHEQ
                     AGLALDSSKHALRRAARAHPRIGAVGADAWGPLP"
     gene            466..1428
                     /locus_tag="VEV43_02070"
     CDS             466..1428
                     /locus_tag="VEV43_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HYP22334.1"
                     /translation="MPRRHALLRATIALLVVAAVAAPASARRARVPKWKSHAPMTHAR
                     TEAAYALAGGKIYVMGGFGPDVVTVPLVEVYDVRSDTWASAPPLPVPVNHAMAAEHDG
                     VVYVAGGYTAVVFGAIPGFFAFEDGAWKPLPPMPETRAAAGMVEHDGKLYVIGGFSQQ
                     GELATTTLVYDVAAGSWSTEPGMPEPREHHMLVEHDGLIYVIGGRIGSPDTNLNDVQR
                     FDPATGEWKELAPMRQPRSGHTCAVTANDLIACLGGEATGAVFDSAEAYDVKRNRWVA
                     LPPLPSGRTGFGSVAVGTKLYSFSGASDAGYLATMESLDLRPYR"
     gene            complement(1425..1820)
                     /locus_tag="VEV43_02075"
     CDS             complement(1425..1820)
                     /locus_tag="VEV43_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HYP22335.1"
                     /translation="MAAIPDDLKDLLDTTALAHVATVTPKGTPRTSPMWFDWDGEHVL
                     LSHTKARAKYRDVQANPRIALSIADPENPYRYLEIRGPVEIEDDPDKTLIHRLAKKYR
                     GVDRYEWDGPGDHRVIFRVTPERVVPRGG"
     gene            complement(1833..2201)
                     /locus_tag="VEV43_02080"
     CDS             complement(1833..2201)
                     /locus_tag="VEV43_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HYP22336.1"
                     /translation="MASVRDEILAGIVQDERPWGGFRQFAHNALCTVKILTVADGQLL
                     SLQSHRHRDELWVVLDEGLQIEIDGEKHVSHPGDEFVIPRGAKHRVGSIGGTGRILEI
                     SFGEFDEDDIERYEDVYGRS"
     gene            2252..2656
                     /locus_tag="VEV43_02085"
     CDS             2252..2656
                     /locus_tag="VEV43_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYP22337.1"
                     /translation="MADPREVLERSGTIAVVGIARTPDKPSGGVPAELQGRGFRIVPV
                     NPKADEILGERAYAQLTDVTEPVDVVEVFRPADEAPEIARQAVAIGAKALWLQLGIVS
                     EEAKRIAQEAGLDFVQDHCMAVESRRHDIRKA"
     gene            complement(2653..3282)
                     /locus_tag="VEV43_02090"
     CDS             complement(2653..3282)
                     /locus_tag="VEV43_02090"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYP22338.1"
                     /translation="MQLREIDPARLEGREPYFLITSLVVPRPVAWVSSLSAEGVLNVA
                     PHSYFNVISSNPWIVHFTSTGEKDTLRNVRATGEFVINVVTRELVEEMNFTSADFPPD
                     ESEFEWAGLEPAPSERVAPPRVARARVAFECRVNEIVSKGNGHMVFGDVLVAHVDEAL
                     FVEGRVDPHLLRPVGRLGGALYTDASGDIFKLPRPTWAQVQSGEVEPPR"
     gene            complement(3272..>3808)
                     /locus_tag="VEV43_02095"
     CDS             complement(3272..>3808)
                     /locus_tag="VEV43_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP22339.1"
                     /translation="DLIPLGRAVAPGAALLSPRGQVLENGMPRFFRRLAMGVFDVEDL
                     KARTEDLAAFVEGAAAHYEIDLTRTILLGYSNGANIGASLLLSRPGLVRAAALLHAMV
                     PFRPDETPALAGTRVLITGGEQDPMIPLDQTHELAEILKAGGAEVELSLEPGGHQLTN
                     SEVGKVAEWVRRIAADAA"
BASE COUNT          561 a         1382 c         1319 g          546 t
ORIGIN      
        1 gcggaagcgg cccccaggcg tcggcaccca ctgcgccgat gcgcggatgg gcccgcgcgg
       61 cgcgccggag cgcgtgcttc gaggagtcga gcgcgaggcc ggcctgctcg tgcgccagca
      121 cgccggcgag gtggtggccg gtgcccgcgc ccaggtcgac gaccggcccg gccggatcga
      181 cgtgccgtgc caccgcctcg gtgatcggcc tgtagtgccc ggcgccgaga aaagcctcgc
      241 gcgcggcgac catctcagcc gaatccccgg cgcccgcggt aagcgtgacg tacccctgcc
      301 gcgcgacgtc gaacgaatgc cccgcttcgc accgcacgga gcccccggcc gccgacaccg
      361 cgcccccgca cacgacgcac gccaggtcgt cgatcacgtc cgtcagcacg aggccattgt
      421 gggcgtcccg caggagcccg ctctgctcgg tcgaaccgcc acgtcgtgcc ccgacgccac
      481 gcgcttctgc gcgccaccat cgccctgctc gtcgtggccg ccgtcgcggc ccccgcctct
      541 gctcgacggg cgcgcgtccc gaagtggaag agccacgccc ccatgacgca cgcacgcacc
      601 gaggccgcct acgcgctcgc cggcggcaag atctacgtga tgggcgggtt cggccccgac
      661 gtcgtgacgg tgccgctggt cgaggtctac gacgtgaggt ccgacacgtg ggcgagcgct
      721 ccgccgctcc cggtaccggt caaccacgcc atggccgccg agcacgacgg cgtcgtctac
      781 gtcgcgggcg ggtacaccgc ggtcgtcttc ggtgcaatcc cggggttctt cgcattcgag
      841 gacggcgcgt ggaagccact tcccccgatg cccgagaccc gcgccgctgc gggcatggtc
      901 gagcacgacg gcaagctcta cgtgatcggc gggttcagcc agcagggaga gctcgcgact
      961 acgacgctcg tgtacgacgt cgcggcgggg tcctggtcga ccgagccggg gatgcccgag
     1021 ccgcgcgagc accacatgct cgtcgagcac gacggattga tctacgtgat cgggggccgg
     1081 atcggcagcc ccgacacgaa cctgaacgac gtccagcgct tcgaccccgc gaccggcgag
     1141 tggaaggagc tcgcgccgat gcgtcagccg cgcagcggcc acacctgcgc cgtcacggcc
     1201 aacgacctga tcgcatgcct gggcggcgag gcgaccggcg cggtgttcga ctccgccgag
     1261 gcctacgacg tgaagcggaa ccgctgggtg gcgctgcccc cgctgccgtc gggccgcacc
     1321 gggttcggaa gcgtcgccgt ggggacgaag ctgtactcgt tctcgggcgc gagcgacgcc
     1381 ggctacctcg cgacgatgga gtcgctggac ctcaggccct accgctagcc gccccgcggc
     1441 accacgcgct ccggcgtcac ccggaagatc acgcggtggt cgccgggccc gtcccactcg
     1501 tagcggtcga cccccctgta cttcttggcc aggcggtgga tcagcgtctt gtccgggtcg
     1561 tcctcgatct cgaccgggcc cctgatctcg aggtagcgat aggggttctc cgggtcggcg
     1621 atcgacaggg cgatgcgcgg gttcgcctgc acgtcgcggt acttcgcgcg cgccttcgtg
     1681 tgcgagagca ggacgtgctc gccgtcccag tcgaaccaca tcggcgacgt ccgcggcgtg
     1741 cctttcggcg tcaccgtcgc gacgtgcgcg agcgcggtcg tgtccagcag gtccttgagg
     1801 tcgtcgggta tcgcagccat gtccccctcc gtctaagagc gtccgtatac gtcttcgtag
     1861 cgctcgatgt cgtcctcgtc gaactcgccg aacgagatct cgaggatccg gcccgtgccc
     1921 ccgatcgacc cgacgcgatg cttggcgccg cgcgggatga cgaactcgtc gcccgggtga
     1981 gatacgtgct tctcgccgtc gatctcgatc tggaggccct cgtccagcac gacccagagc
     2041 tcgtcgcggt ggcggtgtga ctggaggctc aggagctgcc cgtccgcgac cgtcaggatc
     2101 ttcaccgtgc acagcgcgtt gtgcgcgaac tgacgaaagc ccccccaggg gcgctcgtcc
     2161 tgcacgatgc cggcaaggat ctcgtcgcgt accgatgcca tccccgccac cctaagggaa
     2221 tggacacggg ggccgcgcgg ttccatgcga catggcagat cccagagagg tcctcgagcg
     2281 ttccggcacg atcgcggtcg tcggcatcgc gcgcaccccc gacaagccga gcggcggcgt
     2341 ccccgccgag ctccagggcc ggggcttccg catcgtcccc gtgaacccca aggcggacga
     2401 gatcctcggc gagcgcgcct acgcgcagct gaccgacgtc accgagcccg tcgacgtcgt
     2461 cgaggtgttc cggcccgcgg acgaggctcc ggagatcgcg cggcaggccg tcgcgatcgg
     2521 tgcaaaggcg ctgtggctac agctcggcat cgtctccgaa gaggcgaagc ggatcgccca
     2581 ggaggccggg ctcgacttcg tgcaggacca ctgcatggcc gtcgagagcc gccgccacga
     2641 catccgcaag gcctagcgcg gcggctccac ctcgccgctc tggacctgtg cccaggtggg
     2701 gcgcgggagc ttgaatatgt cgcccgacgc gtccgtgtag agcgcgccgc cgagacgccc
     2761 gaccggacgg agcagatggg ggtcgacgcg gccctcgacg aacagcgcct cgtccacgtg
     2821 cgcgaccagg acgtcgccga acaccatgtg gccgttgccc ttcgagacga tctcgttcac
     2881 ccggcactcg aacgctacgc gggcccgggc gacccgcggc ggagcgacgc gctcggaggg
     2941 tgcgggctcg agccccgccc actcgaactc gctctcgtcg ggagggaagt ccgcggacgt
     3001 gaagttcatc tcctcgacga gctcgcgcgt gaccacgttg atcacgaact cgccggtcgc
     3061 gcggacgttg cgcagcgtgt ccttctcgcc cgtcgacgtg aagtgcacga tccaggggtt
     3121 gctcgagatc acgttgaagt agctgtgcgg cgcgacgttg aggacgccct cggccgacag
     3181 cgacgagacc cacgcgaccg gccgcggcac gacgagggac gtgatgagga agtaaggctc
     3241 gcggccctcg agccgggccg ggtcgatctc cctaagctgc atcggcggcg atccggcgca
     3301 cccactcggc gaccttgccg acctctgagt tggtgagctg atgcccgccg ggctcgagcg
     3361 acagctccac ttcggcaccg cctgccttca ggatctcggc gagctcgtgc gtctggtcga
     3421 gcgggatcat cgggtcctgc tctcccccgg tgatcagcac ccgggtgccc gctagggcgg
     3481 gcgtctcgtc cgggcggaac gggaccattg cgtgcaggag cgcggcggcc cggacgaggc
     3541 cgggcctcga cagcagcagg cttgcgccga tgttcgcccc gttcgagtag ccgagcagga
     3601 tcgtccgcgt gaggtcgatc tcgtagtgcg cggcggcccc ctcgacgaag gcggccaggt
     3661 cctcggtccg cgccttcagg tcctcgacgt cgaacacgcc catggcgagg cggcggaaga
     3721 aacgaggcat cccgttctcg agaacttggc cacggggact gagcaacgcc gcccccggag
     3781 cgaccgcacg ccccagcggg atcaggtc
//
LOCUS       DASQDO010000065         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_558312, whole genome shotgun sequence.
ACCESSION   DASQDO010000065 DASQDO010000000
VERSION     DASQDO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558312"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VEV43_02100"
     CDS             <1..1255
                     /locus_tag="VEV43_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP22340.1"
                     /translation="IFTVVIDGGGWNALQYHPDSCPTIKRLMDEGVTYVNATIGSAPS
                     ITGALHATFGTGVYPRTHGLPGHVMRDGEGNLVDVYLDEADPQFLDAPTVSEIWDVSN
                     ANRPIVGTVSFEGWHLGMIGHGAQRQGGDKDVAVLWERDEETWWTNEEYYSLPPGLEE
                     FDEARLAAYEHELDERDGLTDDAWFGHTIEELREPAIRPGTPAFTRLTGDLIVDYIVD
                     SELGRDAVTDLFWIEMKMPDHAGHSWNMVRPEQADVLRETDAQVARMKAALDRVVGPD
                     NYLLVLSADHGQQPLPDLHGGWRINTGEVRADIEERFGKVVEKVTPADIFLDMDAMAE
                     EGWEPEDVARFLGTYTVADSIPEGQPGADLVPEGRLDDRVFAAAFSTGFLESLDPVAD
                     PDRFDSFGKGMYPESSFVVPPEGDE"
     gene            1265..2551
                     /locus_tag="VEV43_02105"
     CDS             1265..2551
                     /locus_tag="VEV43_02105"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP22341.1"
                     /translation="MLKRGLALLMGNRDFRSLMGAQFFAQAGDGLVQVALAASIVFGG
                     QKGFDLEGAKSPDELLRIALYIFVPYTILSPFLGVVIDRWDRRRLLMAANGLRAVVIA
                     VIALAGVGSLPDAVVFGAFLLTLSSTRVVLATKSAALPDTLEGSSLVEANAVSQLGGA
                     LFQLGGAGIALIAKGSIDAEPIVLFGALVYLVGAAFPLSMRSMDPDRQKSSLGAEVAH
                     VARSIAQGLREVARTPKAGASITTYFWLRFLWSFVLVGIGFIARELLADDDLQIAILT
                     GGAGAVGAALGFLSASKLLQRVASTAHLVLSASVVAGVAVTVLGALEMKLAIAALAFF
                     LGFGFFLAKISLDSLVQEALGADFRGRAFSLYDIAYNLAWVLAAGIMKVAWSDDSKGL
                     LLAGAGLVFLAGMAVIGAWFKRAGLLRTADAPAAAR"
     gene            2593..>3245
                     /locus_tag="VEV43_02110"
     CDS             2593..>3245
                     /locus_tag="VEV43_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP22342.1"
                     /translation="MSLGAIALLVYDEIERGPVPSFEEQACALPKAWLERTRRGFYEP
                     RSGQISLLPKTPAYMASGGDGWSHSGPWPYLQDVPLVFYGPGIIPSYAEVTEPATTAD
                     VAPTMAELIGTRFDAPDGEVLGEATSLPRTPRPRLILTIVLDGGGWNTLDMYEGDWPN
                     LARLMAAGVSYRATVGSSPSVTPAVHSTLGTGAYPWKAGVTGVPVRDEDGEVVDAFL"
BASE COUNT          450 a         1115 c         1166 g          514 t
ORIGIN      
        1 catcttcacg gtcgtgatcg acggcggcgg gtggaacgcg ctccagtacc accccgattc
       61 gtgtccgacc atcaagcggc tgatggacga gggcgtgacg tacgtcaacg ccacgatcgg
      121 ctcggccccc tcgatcaccg gcgcgctgca cgcgacgttc gggaccggcg tctacccgcg
      181 cacgcacggg ctccccggcc acgtcatgcg cgacggcgag gggaacctcg tcgacgtgta
      241 cctcgatgag gccgacccgc agttcctcga cgcgccgacg gtctcggaga tctgggacgt
      301 gtcgaacgcg aaccgcccga tagtcggcac cgtctcgttc gagggctggc acctcgggat
      361 gatcggccac ggtgcgcaac gccagggcgg cgacaaggac gtcgcggtgc tgtgggagcg
      421 cgacgaggag acctggtgga cgaacgagga gtactactcg ctgcccccgg gcctcgagga
      481 gttcgacgag gcgcggctcg cggcgtacga gcacgagctc gacgagcgcg acggcctcac
      541 cgacgacgca tggttcggcc acacgatcga ggagttgcgc gagccggcga tcaggccggg
      601 gacccccgcg ttcacgcgtc tgaccggtga cttgatcgtc gactacatcg tcgacagcga
      661 gctcggcagg gacgcggtca cggacctgtt ctggatcgag atgaagatgc ccgaccacgc
      721 cggccactcg tggaacatgg tgaggccgga gcaggccgac gtcctcaggg agacggacgc
      781 gcaggtcgcg cggatgaagg ccgcgctcga cagggtcgtg ggccccgaca actacctgct
      841 cgtgctgagc gcggaccacg gccagcagcc gctgcccgac ctccacgggg gctggcgcat
      901 caacaccggc gaggtgcggg cggacatcga ggagcggttc ggcaaggtcg tcgagaaggt
      961 gacgccggcc gacatcttcc tcgacatgga cgcgatggcc gaggagggat gggagcccga
     1021 ggacgtcgcg cgcttcctcg gcacgtacac cgtcgccgac agcatccccg agggccagcc
     1081 cggcgcggac ctcgtgcccg agggccgcct cgacgaccgc gtgttcgcag cggcgttctc
     1141 gacggggttc ctcgagtcgc tcgacccagt cgcggacccg gaccggttcg actcgttcgg
     1201 caagggcatg tatccggaga gctcgttcgt ggtcccgccc gagggcgacg agtagggtgc
     1261 ggccgtgctg aagcgaggcc tcgcgctgct gatggggaac cgcgacttca ggtcgctgat
     1321 gggtgcgcag ttcttcgcgc aggccggcga cgggctcgtc caggtcgcgc tcgccgcgag
     1381 catcgtcttc gggggccaga agggcttcga cctcgaaggt gcgaagtcgc ccgacgagct
     1441 cctgaggatc gccctctata tcttcgtccc gtacacgatc ctgagcccgt tcctgggcgt
     1501 cgtcatcgac cggtgggacc gccgccggct gctgatggcc gcgaacgggc tgcgggccgt
     1561 ggtgatcgcg gtcatcgctc tcgcgggggt cgggtcgctg cccgacgcgg tcgtcttcgg
     1621 ggcattcctt ctgacgctga gctcgacgcg ggtcgtgctg gcgaccaagt cggcggccct
     1681 tccggacact ctggaaggtt cgtctctcgt cgaggccaac gcggtgtcgc agctcggagg
     1741 cgcgctgttc cagctcggcg gcgcggggat agcgctgatc gcgaagggga gcatcgacgc
     1801 ggagcccatc gtcctgttcg gcgcgctcgt ctacctcgtc ggcgccgcgt tccccctgtc
     1861 catgcggtcg atggaccccg accgtcagaa gtcgagcctc ggcgccgagg tcgcgcacgt
     1921 cgcacgctcg atcgctcagg gcctgcgtga ggtggcgcgc accccgaagg ccggcgcgtc
     1981 catcaccacc tacttctggc tccgcttcct gtggagcttc gtgctcgtcg gcatcggctt
     2041 catcgcgcgc gagctgctcg ctgacgacga cctccagatc gcgatcctca ccggtggcgc
     2101 gggtgccgtg ggggccgcgc tcggcttcct ctccgcgagc aagctcctcc agcgggtcgc
     2161 ctcgacagca cacctggtcc tgtcggcgtc ggtcgtcgcc ggggtggcgg tcacggtcct
     2221 cggagccctc gagatgaagc tcgcgatcgc ggccctcgcc ttcttcctcg gcttcggctt
     2281 cttcctggcg aagatctcgc tcgactccct cgtgcaggag gcgctgggag ccgacttccg
     2341 cggccgcgcg ttctccctct acgacatcgc ctacaacctc gcctgggtcc tcgcggccgg
     2401 gatcatgaag gtcgcgtgga gcgacgactc caagggcttg ctgctcgcgg gggccgggct
     2461 cgtcttcctc gcagggatgg ccgtgatcgg cgcctggttc aagagagcgg gtctgctacg
     2521 gacggcggac gccccggccg cagcgcgcta gcgcgccggg tcgcggcggc ggtcaccgcg
     2581 ctcgtcgtcg cggtgtcgct cggggccatc gcgctgctgg tgtacgacga gatcgagcgt
     2641 ggcccggtcc cgtctttcga agagcaggcc tgcgcgctgc cgaaggcatg gctcgagcgc
     2701 acgcggcgcg gcttctacga gccgcgttcc ggccagatct cgctgctgcc caagacgccc
     2761 gcgtacatgg cgagcggagg tgacggctgg agccattccg ggccgtggcc gtacctccag
     2821 gacgtgccgc tcgtgttcta cgggccgggg atcatcccgt cgtacgccga ggtgaccgag
     2881 ccggctacga ccgcggacgt cgcgccgacg atggccgagc tgatcgggac gcgcttcgac
     2941 gcccccgacg gcgaggtgct cggggaggcg acgtcgctgc cccgtacgcc gcgcccgcgt
     3001 ctgatcctca cgatcgtgct cgacggcggc gggtggaaca cgctcgacat gtacgagggg
     3061 gactggccga acctggcgag attgatggcg gccggcgtgt cctaccgggc gaccgtcggg
     3121 tcctcgccgt ccgtgactcc ggcggtgcac tcgacgctcg ggaccggcgc ctacccgtgg
     3181 aaggccggcg tcacgggcgt ccccgtgcgc gacgaggacg gcgaggtcgt cgacgcgttc
     3241 ctcga
//
LOCUS       DASQDO010000066         5081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1170206, whole genome shotgun sequence.
ACCESSION   DASQDO010000066 DASQDO010000000
VERSION     DASQDO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170206"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1905
                     /gene="dnaG"
                     /locus_tag="VEV43_02115"
     CDS             97..1905
                     /gene="dnaG"
                     /locus_tag="VEV43_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HYP22343.1"
                     /translation="MAKIKDDDIDALRERADLVEVVSAHSQRKRAGSHTFKGLCPFHS
                     EKTPSFQVDAAKGLWHCFGCGEGGNVYHFVQKTENLPFPEAVEWLARRFAFELHYEES
                     RPGEHSAQRGVKARIVEANDLAARFFNQQLFTTPDAAPARTYLESRGFGRDVAERWQL
                     GYAPGRNALARHLLSKGFKQEEIVQADLARVSDRDGSLYDTFRQRITFPTWNLQGDVV
                     GFGARALGDQQPKYLNTSETPVFSKSRVMYGLNRAKSAIARGAALVVEGYTDVIALHE
                     AGVQEAVATNGVALGDTHFEQLKKFTSRAVLMFDADAAGAGATERGFGIHRRIGIEVL
                     VAPLPAGRDPADVVKSDGDEGIRKVIDAAQPLLEFKLEQTLAKLPLDTPEARAVAVGK
                     AAEVLGWQSDPVVRGEYAYLAAQRIGVDAEVIHRELAGERSLGAGASGGETGPQGDRR
                     FPGHVKVEREALQLLLLRTQEAGPWAKDIEPTHFTSAARRELFARARSWVDVGSAIDE
                     RAFAEQLSPEALSLFTELTVGAGFPTGDDLDARVREVFVRLKVFVLERDIKGRRNTLQ
                     EINPLEDPARHDALFTELVGLEAERRDLLRQVQGAA"
     gene            1906..3189
                     /gene="rpoD"
                     /locus_tag="VEV43_02120"
     CDS             1906..3189
                     /gene="rpoD"
                     /locus_tag="VEV43_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HYP22344.1"
                     /translation="MSDQETNAVTTAPPETPPVEPPAGGTLTDVTPAQLEVDKVIRGA
                     KAEGSITAADLADKLSTLDLNPDETDAVYQRLIDLGVDVVEDEVIEEEEEEEEAVEAV
                     PAPDADEDRVRARREVDMALKAPTNDPVRMYLKEIGRVALLTAQEEVSLAKRIEAGVF
                     ADERLEREAETLPDDQRIELRWTSADGARAKRHLVEANLRLVVSIAKRYVGRGMAFLD
                     LIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLLRIQRQLLQDLGREPTADEIGEQMELPPEKVREIQKISQEPVSLETPIGEEEDSNL
                     GDFIEDSEAVVPLERASFRLLQEQLESVLHTLSDGEKEVIRLPFGLVDGQPCTLEEVG
                     KKFGVTRERIRQIESKTLSKLRHPSRSHKHRDYLE"
     gene            complement(3202..3471)
                     /locus_tag="VEV43_02125"
     CDS             complement(3202..3471)
                     /locus_tag="VEV43_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HYP22345.1"
                     /translation="MRGRLGVLIGFGAGYVLGAKAGRERYEQLQRLYENVVSSPGFQQ
                     ATGKAKEAVGTGLEQAKDLASEGVNKVKGNDSRPSGLSVAPPPQP"
     gene            3574..3646
                     /locus_tag="VEV43_02130"
     tRNA            3574..3646
                     /locus_tag="VEV43_02130"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3607..3609,aa:Asn,seq:gtt)
     gene            3712..4185
                     /locus_tag="VEV43_02135"
     CDS             3712..4185
                     /locus_tag="VEV43_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22346.1"
                     /translation="MGERRDLDLYGSGATPGRTPVTAWIVRIAGLLVGAAITLYGVAL
                     YALRCFDTCPEDPAVNTATVLMSAAIVLFGPAVAIASVSVRTRWAAGGATVVAALGFA
                     IVAVGVASLAQVPRIDDPGGQKADVTLAGAAETVVGAGVTAAALLRRKRGPRSAD"
     gene            4377..5030
                     /locus_tag="VEV43_02140"
     CDS             4377..5030
                     /locus_tag="VEV43_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP22347.1"
                     /translation="MNHHQSDVDVDQLWADYGADPSSKNREALIRNYLPLVEFLAGRL
                     GRHVHASYRADLYSFGVEGLMDAIDKFRPEMGNRFETYGSCRIRGAMSDGIRKMSWLP
                     RGAENRSSRVIETVVPVDFQAARTTVGARLQDCLSDRTAGTPFDGLLLEADHAEVAAA
                     IRGLPERERNVVFEHYYAEKQLKEIGRDMGVTESRVCQLHRRALRMLEGALGEPATA"
BASE COUNT          844 a         1749 c         1763 g          725 t
ORIGIN      
        1 ctgggggtcc tacaatgagt ccacttcccc ttccttcatg agccgcgccc gtaagggggc
       61 gcgggtcgcc tcggcgagac gcccagagga ggtgggatgg ccaagatcaa agacgacgac
      121 atagacgctc tgcgcgagcg cgctgacctc gtcgaggtcg tctcggccca ttcccagcgc
      181 aagcgcgccg gctcgcacac gttcaagggg ctctgcccct tccactccga gaagacgccg
      241 tcgttccagg tcgacgcggc gaaggggctg tggcactgct tcgggtgcgg cgagggcggc
      301 aacgtctacc acttcgtcca gaagaccgag aacctgccgt tccccgaggc ggtcgaatgg
      361 ctcgcccggc ggttcgcgtt cgagctccac tacgaggagt cccggcccgg cgagcactcg
      421 gctcagcggg gcgtcaaggc gcggatcgtc gaggcgaacg acctcgcggc ccgcttcttc
      481 aaccagcagc tgttcacgac tccggacgcg gcccccgccc gcacctacct cgagagccgc
      541 gggttcgggc gcgacgtcgc cgaacggtgg cagctgggct acgccccggg ccgcaacgct
      601 ttggcgcggc atctgctgag caagggcttc aagcaggagg agatcgtcca ggccgacctc
      661 gcccgggtga gcgaccgcga cggctccctc tacgacacgt tccgccagag gatcacgttc
      721 ccgacgtgga acctgcaggg agacgtcgtc gggttcggcg cccgtgctct cggcgaccag
      781 cagccgaagt acctcaacac gtccgagaca ccggtgttct cgaagtcgcg cgtgatgtac
      841 gggctcaacc gtgcgaagtc ggcgatcgca cgcggggccg cgctcgtggt cgagggctac
      901 accgacgtga tcgctctcca cgaggccggg gtgcaagagg ccgtcgcgac gaacggcgtc
      961 gcgctcggcg acacgcactt cgaacagctc aagaagttca cctcgcgggc ggtgctgatg
     1021 ttcgacgccg acgcggccgg cgcgggtgcg accgaacgcg gtttcggcat ccatcgccgg
     1081 atcgggatcg aggtgctcgt cgcgccgctg ccggcgggac gagacccggc cgacgtcgtc
     1141 aaatccgacg gcgacgaagg catccgcaag gtgatcgacg ccgcgcagcc gctgctcgag
     1201 ttcaagctcg aacagacgct ggcgaagctc ccgctcgaca cgccggaagc gcgtgccgtc
     1261 gccgtgggca aggcagccga agtgctaggg tggcagtccg atccggtggt gcgcggcgag
     1321 tacgcatacc tcgccgcgca gcggatcggg gtggacgccg aggtcatcca cagggagctc
     1381 gcgggggagc gctccttggg ggccggggcg tcggggggtg aaacgggtcc gcaaggtgac
     1441 cgtcgcttcc ctggacacgt gaaggtcgag cgggaggcgc tccagttgct gcttctacgg
     1501 actcaggaag cgggtccctg ggccaaggac atcgagccca cccacttcac atcggctgcg
     1561 cgacgcgagc tgttcgcgcg tgcgcgctcg tgggtagacg tcgggagtgc gatcgacgag
     1621 cgtgctttcg cggagcagct gtcgcccgaa gccctgtcgt tgttcaccga gctcacggtg
     1681 ggcgccggct tcccgaccgg cgacgacctc gacgcgcgcg ttcgtgaggt cttcgtgcgg
     1741 ctaaaggtgt tcgtcctcga gcgcgacatc aagggccgcc gcaacaccct gcaggagatc
     1801 aacccgctcg aagaccccgc gcgtcacgac gcgctgttca ccgagctggt cgggctggaa
     1861 gccgaacgcc gcgacctgct ccgccaggtc cagggggccg cgtagatgag cgaccaggaa
     1921 acgaacgcag tcacgaccgc gccgcccgag acgccgccgg tcgagccacc cgcgggtgga
     1981 acgctcaccg acgtcacccc ggcgcagctc gaggtcgaca aggtcatccg cggcgccaag
     2041 gccgaaggat cgatcacggc cgcggacctc gccgacaagc tgtcgacgct cgacctcaac
     2101 cccgacgaga ccgacgcggt gtaccagcgt ctgatcgacc tcggcgtcga cgtcgtcgag
     2161 gacgaggtga tcgaagagga agaggaagaa gaagaggccg tcgaggccgt gcccgccccc
     2221 gacgccgacg aggaccgcgt gcgcgcaaga cgcgaggtcg acatggccct caaggcgccg
     2281 acgaacgacc ccgtccgcat gtacctgaag gagatcgggc gcgtcgcgct gctgaccgcg
     2341 caagaggagg tctcgctcgc gaaacggatc gaggccggcg tgttcgcgga cgagcgcctc
     2401 gagcgcgagg ccgagacgct tcccgacgac cagcgcatcg agctccgctg gacgagcgcc
     2461 gacggtgccc gcgccaagcg ccacctcgtc gaggcgaacc tgcggctcgt ggtctcgatc
     2521 gcgaagcgct acgtgggccg cggcatggcg ttcctcgacc tgatccagga agggaacctc
     2581 gggctcatcc gcgcggtcga gaagttcgac tacacgaagg gctacaagtt ctcgacgtac
     2641 gcgacgtggt ggatacgcca ggcgatcacg cgtgcgatcg ccgaccaggc ccgcacgatc
     2701 cgcatcccgg tccacatggt cgagacgatc aacaagctcc tgcgcatcca gcgtcagctg
     2761 ctccaggacc tcggccgcga gccgaccgcc gacgagatcg gcgaacagat ggagctcccg
     2821 cccgagaagg tgcgcgagat ccagaagatc agccaggagc cggtgtcgct cgagaccccg
     2881 atcggcgagg aagaggactc gaacctcggc gacttcatcg aggactcgga ggccgtggtc
     2941 ccgctcgagc gggcgtcgtt ccggctacta caggagcaac tcgagtcggt cctccacacg
     3001 ctgtcggacg gcgagaagga agtgatccga ctgcccttcg gcctcgtcga cggccagccc
     3061 tgcacgctcg aagaggtggg caagaagttc ggcgtgacgc gcgagcggat ccgccagatc
     3121 gagtcgaaga cgctgtcgaa gctgcggcac ccctcccgga gccacaagca ccgggactac
     3181 ctcgagtagg gcgctgccgc cttagggctg cggcggggga gcgaccgaca ggcccgacgg
     3241 gcgcgagtcg ttgcctttca ccttgttcac gccctcgctc gccaggtcct tcgcctgctc
     3301 caggccagtc cccacggctt ccttcgcctt gcccgtcgcc tgctggaagc ccggcgagct
     3361 cacgacgttc tcgtagaggc gctggagctg ctcgtaacgc tcccggccgg ccttggcgcc
     3421 gagcacgtag ccggctccga acccgatcag gacaccaaga cgaccgcgca tgacgacctc
     3481 ccgaaagatc agcgatgtga tgccagatcg tatgcccggt atgccgtacg gcaaatcccg
     3541 gcccccggcc cctataggat gacgcggttc cgatccgggg tagctcaacc ggcagagcaa
     3601 ccggctgtta accggtaggt tgcgggttcg agtcccgccc ccggagccca cgcatcgaga
     3661 ggcggcccgc cgaggccgcc tttctcgcgc ccggcccttt accatcgcgt cgtgggcgag
     3721 cggcgggacc tggacctcta cgggagcggg gcgactcccg gccgtacgcc cgtgacggcc
     3781 tggatcgtgc ggatagccgg gctcctggtg ggggccgcga tcacgctcta cggcgtggcg
     3841 ctctacgccc tgcgctgctt cgacacctgc cccgaggacc ccgcggtgaa caccgcgacc
     3901 gtactgatgt cggcggcgat cgtgttgttc ggcccggccg ttgcgattgc gtcggtgtcc
     3961 gtgcgcacgc gctgggcggc cggcggagcg acggtcgtgg ccgccctcgg gttcgcgatc
     4021 gtcgccgtgg gggtcgcctc gctcgcccag gtgccgagga tcgacgaccc cggcggccag
     4081 aaagccgacg tcacccttgc gggggccgca gagaccgtcg tcggagccgg cgtgaccgcg
     4141 gcggccctcc tccggcgcaa gcgagggccc cgctcagccg actagccccc cgactacgcc
     4201 cccaccaggg atttcgcgct ctccgccatc ccatctagct agtcaaggac cactcccaac
     4261 ttccccttcg agggggaacc gttgcacccc tcagggggat gcggccccag cgccccggct
     4321 gtcagggtcg gttgagcttg ttgcttgttg ttgcttattg gggctgggag gccgggatga
     4381 accaccatca atcggacgtc gacgtggacc agctatgggc cgattatggg gccgatccct
     4441 cgagcaagaa ccgcgaggcg ctgatccgca actacctgcc gctcgtcgag ttcctcgccg
     4501 ggcgactcgg ccgccacgtc cacgcctcct accgggcgga cctctacagc ttcggggtcg
     4561 agggcctcat ggacgccatc gacaagttcc gccccgagat gggcaaccgc ttcgagacct
     4621 acggctcgtg ccggatccgc ggcgcgatga gcgacgggat ccgcaagatg tcgtggctgc
     4681 cgaggggggc cgagaaccga tcgagccgcg tgatcgagac cgtcgtcccg gtcgacttcc
     4741 aggccgcgcg cacgacggtc ggcgcgaggc tccaggactg cctcagcgac cgcaccgcgg
     4801 gtacgccgtt cgacggcctg ctcctcgagg cggaccacgc ggaggtcgcc gcggcgatcc
     4861 gcggtctgcc cgagcgcgag cgcaacgtcg tgttcgagca ctactacgcc gagaagcagc
     4921 tgaaggagat cgggcgggac atgggcgtca ccgagagccg ggtctgccag ttgcaccgca
     4981 gggctctgcg gatgctcgaa ggagcgttgg gcgagccggc gactgcgtaa ggagcgccgg
     5041 cacgggggag tgagcgcggc ggtggcccgg aggggcgccg g
//
LOCUS       DASQDO010000067         1704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1048068, whole genome shotgun sequence.
ACCESSION   DASQDO010000067 DASQDO010000000
VERSION     DASQDO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048068"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VEV43_02145"
     CDS             <1..1322
                     /locus_tag="VEV43_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYP22348.1"
                     /translation="PVPAEARARESRLRRLLTPLMIASVVTVPLLVPPIGPNSVPADF
                     FNAAFLILGLAVLARTRVAARIPMGGSYLLIITGGLIAVTQSVVPKESLIAVVQDAYL
                     FVWFVVAVNYLISEDGRRTRWIASVWGVVGVVMAFVVWFARFGYPYDVPVLFDYPLVD
                     AWGRSEGTFRDPNMAGNYLVLSLFVLWAAPRPRSVALKLLMTVPFLFAVYHTQSITAL
                     VTLAAGALVAFAVSFVARREAVVAAAMAVVAAGLAALVILPQTFLSRSGEVATQLGET
                     EVFDESLGRSNTSLTLRAARWEEAFQLFGSSIVLGIGPSSTDESLADRGAPLTGEIHN
                     DYVAGLIERGLVGALGNLFLFAVAAGWALRVAIDRRRRLEGWRPAALAGGMVVVLLAA
                     VSLETLHFRHVWLFFALVVALGMTGPSSRSGRPARAPDTSVRASTRS"
     gene            complement(1258..>1704)
                     /locus_tag="VEV43_02150"
     CDS             complement(1258..>1704)
                     /locus_tag="VEV43_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22349.1"
                     /translation="TPSPEPSPSATPVATEVPDASPILRPTEPLWPAAGGRGGRADRK
                     PPSRPKDLLSAALETGSYLWWDSASDDRGVVGYAIWRDGRRIGRTRSTSFEDLKGRGS
                     RHVYAVRAFDAAGNRSKAAVLRVRTVRNASTLARTCLVPSRVYRTC"
BASE COUNT          196 a          608 c          609 g          291 t
ORIGIN      
        1 ccccggtccc ggccgaggcc cgcgcgcgcg agagccggct ccgccgcctg ctgacgcctc
       61 tgatgatcgc gagcgtcgtg accgtgccgc tgctcgtgcc cccgatcggc ccgaactccg
      121 tcccggccga cttcttcaac gccgcgttcc tgatcctcgg cctcgcggtc ctggctcgca
      181 cgagggtcgc cgcccggatc ccgatgggtg ggagctacct gctcatcatc acgggcggac
      241 tgatcgcggt gacgcagtcc gtcgtcccga aggagtcgct gatcgcggtc gtccaggacg
      301 cgtacctctt cgtgtggttc gtcgtcgccg tcaactacct gatctcggaa gacggtcgtc
      361 ggacgcgctg gatcgcgtcg gtctggggcg tcgtcggcgt cgtgatggcg ttcgtcgtgt
      421 ggttcgcgcg cttcggctac ccgtacgacg tcccggtgct gttcgactac ccgctggtgg
      481 acgcgtgggg ccggtccgag gggacgttcc gcgacccgaa catggccggc aactacctcg
      541 tcctgtcgct gttcgtcctg tgggccgcgc cgcggccgcg gagcgtcgcg ctgaagctcc
      601 tgatgaccgt cccgttcctg ttcgccgtgt accacacgca gtcgatcacg gcactggtga
      661 cgctcgccgc cggcgcgctc gtcgcgttcg ccgtctcgtt cgtggcgcgg cgtgaagccg
      721 tcgtcgccgc ggccatggcc gtcgtcgcag cgggtctggc ggctctggtg atcctcccgc
      781 agacgttcct gtcgcgcagc ggcgaggtcg ccacgcagct cggcgagacc gaggtgttcg
      841 acgagtcgct gggccggagc aacaccagcc tgaccttgcg cgcggcccgg tgggaggagg
      901 cgttccagct cttcggcagc agcatcgtcc tgggcatcgg cccctcgtcg accgacgagt
      961 cgctcgccga caggggggcg ccgctgacgg gcgagatcca caacgactac gtcgcgggcc
     1021 tcatcgagcg cgggctcgtc ggcgcgctcg gcaacctctt tttgttcgcg gtggccgcgg
     1081 ggtgggcgtt acgcgtggcg atcgacaggc gaaggcgcct cgagggatgg aggccggcgg
     1141 ctctcgccgg cggcatggtc gtcgtgttgc tggcggcagt gagcctcgag acgctccact
     1201 tccgtcacgt ctggctgttc ttcgccctcg tcgtggcctt ggggatgacg ggaccgtcta
     1261 gcaggtccgg tagacccgcg agggcaccag acacgtccgt gcgagcgtcg acgcgttcct
     1321 gaccgtccgc acccgcagga ccgccgcctt cgagcggttg ccggcagcgt cgaaggcgcg
     1381 cactgcgtag acgtggcggc tgccgcggcc cttcaggtcc tcgaagctcg tcgagcgcgt
     1441 ccggccgatc ctgcgaccgt ccctccagat cgcgtagccc acgacgccgc ggtcgtcact
     1501 ggccgagtcc caccacaggt acgagccggt ctcgagagca gccgacagga ggtccttggg
     1561 ccgcgacggc ggcttgcggt cggcccggcc cccgcgtcca ccggctgccg gccacagcgg
     1621 ctcggtcggg cgcaggatcg gcgaggcgtc cgggacctcg gtcgcaaccg gcgtcgcgct
     1681 cggcgacggc tcgggcgacg gcgt
//
LOCUS       DASQDO010000068         9789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_864527, whole genome shotgun sequence.
ACCESSION   DASQDO010000068 DASQDO010000000
VERSION     DASQDO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864527"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VEV43_02155"
     CDS             complement(<1..784)
                     /locus_tag="VEV43_02155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYP22350.1"
                     /translation="MTTTRLSRAVPALVAAALLAASFRGGVPATASSISADVTSGGGT
                     VAHADGKKLPYKGRQQARFYRIGLPAGEPTIAIAENGDMFFPSIDVDSSPPNHVEVVR
                     SKDEGKTWEIVSPNLAGAANSHPISLDPYVWADRDTDRIFNLDLTVACSLMSFTDDLG
                     ETWTTNPLACGRPVNDHHTLFGGPPATSTTVGYPNVLYYCWNDVASSSCSKSIDGGLT
                     WSATGSPAFTGVQPTAGDDGEPPPFCGGLHGHGVVGRDGAIFL"
     gene            905..1639
                     /locus_tag="VEV43_02160"
     CDS             905..1639
                     /locus_tag="VEV43_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYP22351.1"
                     /translation="MERQEWRVGELARATGLTVRTLHHYDEVGLLKPSGRTPAGHRLY
                     SGDDVRALYSIVALRGLGFSLAEIGALVRDGVDAREATKRRLAELDRQLVVQRRLRHR
                     LAELLEVLERDGEMPPGQLVEIVEEMTMHEKYYTPEQLAELERRREELGGDDAMKRAE
                     QEWSDLMAAVRAEMEKGTDPADPRMQELAARWTGLIEQFTGGDPAVMKSLQTMYDQEG
                     PEKASRGMVDREVMEYAQRAIAARGA"
     gene            complement(1636..4011)
                     /locus_tag="VEV43_02165"
     CDS             complement(1636..4011)
                     /locus_tag="VEV43_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HYP22352.1"
                     /translation="MKGRNLLAFACAGMLLTACEVFPTSMQDDPAAIEPIADMSQEIT
                     ITEPPTSELKERIVFQQGPIRPEIYTMGTNGYQPRRLTKNVQADREPSWSFDAKKVVY
                     RCHVLTFCMSNPDGTGAEQVYTHTEPSATDVGDPKLSPDGRSIAFDAEIFRGGKEATT
                     GDVLVVNLDTKRVTNVSNTPNQEDDDPTWHPTGGLLWSRAGQLAYDADAAPGGEVLTL
                     TDPGTRVTQPDVSPSGNKIAYVARLAGDTQTDVYVFDLTAPAETPDVKVTFTPFNDVA
                     PSWSPNDRKIALASKDQPSDQDLEIFVVDTQPPEGGTATRRRLTFNDTDENEPDWGAP
                     QVTASVQQRLTLAEGNPASGQTTGTTNFTFAVFLSAPVSPGEQVTVRVATLAGSATTG
                     TDYTAVDQTITFDPGEQTKTFTVPVVADAVSETNESFSIKIQQVTNALIGNAEGRAII
                     TNDDVPPATPSPTPTPTSSPTPPPGQAGGQIAYTSGVDGDYDVWIMDGDGTDKANLTS
                     DPASEGSPDLSPDGRTVAFQRMIGSPSGGSPPDFQIYQVPSTGGAATFLNGTPDAFDV
                     TPAWSPDGSSLAWVSSTSTGDPDIVTASPPTASPSVLIDTPDLEGYAEWGRLANGTSA
                     IAYHVQPDDPAGQWQVRLVELPSKTVRSFGEGRHPEISPNGRTLVFASNRAGGDYEIY
                     TLDLTTAGASPTALTNNTVDDFTPSWSPDGTKIAWTSHPNGGRADVFTMNANGSGQQN
                     VTNSPATDDFEPSWGVKPASSSPRETTPATILVMVPLMGAASALAARRKRS"
     gene            complement(4012..5262)
                     /locus_tag="VEV43_02170"
     CDS             complement(4012..5262)
                     /locus_tag="VEV43_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22353.1"
                     /translation="MHFATALRWRVAAALLVSMLGVAALPLVEARAQSVFEVEVGRLI
                     REGDLTKESMRFYPSELQVRQGDVVHFTSVGFHGVALLPLGETAGEWAAANAGGVGRP
                     WSLFEPDADEGPDVLKGNLSAIFPSRDCGWPTEAPCEFDARPHELGDVLGSGLALVPQ
                     GDSAQAHQLDFSVTINADPGEAVEVVDVVNPKMSMRIEVVGEDEPVSDPATLAEQADE
                     VFAQDVATANRLHRSYSRRNATKKVRGKTYRMAWAGVETQTVSLRGMYPRKITIKKGQ
                     GVRWNFNKNVFSAYTVTFPAGRAISQANAFPVIACDPDGDVESEAPDTAPTSAQAPYC
                     ASYAELELEVPAGLASPAGDARLTSAKDIASSGLRGAGLVTDARPYDLLFSKPSPKMG
                     FGYASLLHEISGVRAAGKVVVRRK"
     gene            complement(5356..6123)
                     /locus_tag="VEV43_02175"
     CDS             complement(5356..6123)
                     /locus_tag="VEV43_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22354.1"
                     /translation="MRLPPTAAAAARHSIDMWRLYVDAIPGASLEERDGAALVLTGID
                     TTGFNGVWLERGDVPAEVVDGLLDRAASAAVLHSLHLPSGAAPALREIPRRRGMVFDD
                     EEPLMILEEDTALRRAAEVPGLAVDRLAPGEGADHARVAATAFEAPVAAFEAAATPAL
                     LAVTEIGCYVGRVDGEAVTTAVGVTCRGATGIVSVATLAAHRGRGYGAAVTARAALEG
                     FAAGAGWVWLTSAPKAVPMYARLGFRAVDTISIWGSE"
     gene            6309..7742
                     /locus_tag="VEV43_02180"
     CDS             6309..7742
                     /locus_tag="VEV43_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22355.1"
                     /translation="MPWIVVIPLGVMVLGMMVGFARQFSGAGQRRAKAHGAAGTAGTW
                     LIFVSFVELAIGLGSLEAERRAAGSGGGFSMAATVLLGVGALLFVIGVYLKIRGRRRA
                     RIYNSGVPGEAVIRAVHQTGTMVNNQPMYAFDVEVTGQGFAPVSTRVREVVPFWFLNR
                     VGPQARVPVKVDPQNPTRMIFDWDAMGTMATQVGTPAPAPMASSFGGVQSGQVTTAGG
                     VGQGAQQMMPSADSLASAMQAAREATANAGSGWHVGKVIGLAVTLFVLVVIGGGLFFI
                     ARIFGQVSDVTDSVTDQVSDALDQADGGFGEGFQGGGSAATTIEVTRPAAGGRPVTFQ
                     ATIPVGWVDVTPTGSGERQGPVLVDLVAKPQAPSEARIVVTRSVRYVENPAPPGTGIE
                     SIRSDVEKEFGDSLTGSSSVTLAGEKAVLLEIAPGADGLRSRQIAVMRGGQVLFVGLV
                     APKSEWGSFVSVFDDVVESWKWRKVTT"
     gene            7767..8063
                     /locus_tag="VEV43_02185"
     CDS             7767..8063
                     /locus_tag="VEV43_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22356.1"
                     /translation="MVPEWADAVFVLCGTIVCVVAFRYGAAARPSPERALAAARIGKL
                     VMSAGGVAASIATASVFADGSIPTIGWAMFGLAGIALGSGVALRLGAGWPRTRP"
     gene            complement(8084..8695)
                     /locus_tag="VEV43_02190"
     CDS             complement(8084..8695)
                     /locus_tag="VEV43_02190"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HYP22357.1"
                     /translation="MADIVAYRIAGWDTPFWVSPNRWPRRFNKAGRGPVQYWALHPLG
                     PLAEVLRAQGLRSPDALQELRLRVWVSRVKVRKVEEVNFDNAGDYGLEPQHLISDDYG
                     ACQELGDRCLREGDSPEVLEVPSAALPGTRNLVMFGPRVAVPYLIDPIGPTDVPTSIC
                     AEGSRAPAELLPLVRHLGEPHTEFEAWRTGTAFAFAEPDIGRS"
     gene            complement(8704..9378)
                     /locus_tag="VEV43_02195"
     CDS             complement(8704..9378)
                     /locus_tag="VEV43_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22358.1"
                     /translation="MANSVLDRTAAAALERQLRDANLTLAREKEVPERVEKLVRKIGS
                     DVMNISPQESDAVDPYLLLALHQGALIALFALDVNSSSRKRTMLRVALEKMRQAARDI
                     IEGIPTSEDRSSKDVARWLVEALAVPQAEIAGALSVNGRTFQRWISSSDPSEPRDEEA
                     LKVRVLARVAQNLRHAFSGPGVLAWLERPHPELKGAAPRDLLSKRENFEQLVHLAAAA
                     RSSAST"
     gene            9481..9603
                     /locus_tag="VEV43_02200"
     CDS             9481..9603
                     /locus_tag="VEV43_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22359.1"
                     /translation="MPHSGMCGSAAGGAHHSGVTSSTSGVSVRFTTSLPSGLTV"
BASE COUNT         1402 a         3272 c         3522 g         1593 t
ORIGIN      
        1 gcaggaagat cgctccgtcg cggccgacga ctccgtggcc gtggagcccg ccgcagaagg
       61 gaggcggctc gccgtcgtcg cccgcggtcg gctgcacgcc cgtgaacgcg ggcgagcccg
      121 tcgccgacca cgtgagcccg ccgtcgatcg acttcgagca cgaggacgaa gcgacgtcgt
      181 tccagcagta gtagaggacg ttcggatatc cgacggtcgt gctcgtggcg ggcgggcctc
      241 cgaacagcgt gtggtggtcg ttcaccgggc gcccgcacgc gagcgggttc gtcgtccagg
      301 tctcgccgag gtcgtcggtg aacgacatca gcgagcacgc gaccgtgaga tcgaggttga
      361 agatccggtc ggtgtcccgg tcggcccaca cgtacgggtc gagcgagatc gggtgggagt
      421 tcgcggcccc cgcgaggttc ggcgagacga tctcccacgt cttgccctcg tccttcgacc
      481 gcacgacctc gacgtggttc ggcgggctcg agtcgacgtc gatcgacggg aagaacatgt
      541 cgccgttctc ggcgatcgcg atcgtcggct cgccggccgg aaggccgatc cggtagaagc
      601 gcgcctgctg gcgtcccttg taggggagct tctttccgtc ggcgtgggcg acggtgcccc
      661 cgcccgacgt gacgtcggcg ctgatcgagc tcgcggtcgc cggcactcct ccgcggaagg
      721 acgccgccag aagtgcagcg gccaccaagg ccggcaccgc cctgctcaag cgcgtagtcg
      781 tcatctagcc cgccctcctg ttttcgaacg cgtcgattcg cccgattggg ggaggtttcg
      841 ccaccccgtg tcgggtccct gccgcgcttg cacctcacgt cgcgtgaggg tctagcgtct
      901 cgtcatggaa cggcaggagt ggagggtcgg cgagctcgcc cgggcgaccg gcctcaccgt
      961 gaggacgctg catcactacg acgaggtcgg cttgttgaag ccgtcgggcc gcacccctgc
     1021 cggccaccgt ctctactcgg gcgacgacgt ccgggcgctg tactcgatcg tcgcgttgcg
     1081 agggctcggc ttctcgctcg ccgagatcgg tgcgctcgtc cgggacggcg tcgacgcgag
     1141 ggaggcgacg aagagacgtc tcgccgagct cgaccggcag ctggtcgtgc agcggcgctt
     1201 acggcaccgg ctggcggagc tgctggaggt gctggagcgc gacggcgaga tgccgccggg
     1261 acagttggtc gagattgtgg aggagatgac gatgcacgag aagtactaca cgccggagca
     1321 gctggcggaa ctcgagcggc gccgcgagga gctcggcggc gacgacgcga tgaagagggc
     1381 cgagcaggag tggtcggacc tgatggccgc cgtccgcgcc gagatggaga aggggaccga
     1441 cccggccgac cctcgcatgc aggagctcgc cgcgcgctgg accgggctga tcgagcagtt
     1501 caccggcggc gaccccgcgg tcatgaagtc cctgcagacc atgtacgacc aagagggccc
     1561 ggagaaggcg tcgcgcggga tggtcgaccg tgaggtgatg gagtacgcgc agcgggcgat
     1621 cgcagcccgc ggcgcttagc tcctcttcct gcgagctgcg agggccgacg cggcccccat
     1681 cagcgggacc atcacgagga tcgtcgccgg cgtcgtctct cgcggtgagg acgacgccgg
     1741 cttcacgccc cacgagggct cgaagtcgtc ggtggccggg ctgttcgtga cgttctgctg
     1801 acccgagccg ttcgcgttca tcgtgaacac gtcggcgcgg cccccgttcg ggtggctcgt
     1861 ccaggcgatc ttcgtcccgt ccggcgacca cgacggcgtg aagtcgtcga ccgtgttgtt
     1921 cgtcagcgcg gtcggcgacg cgcccgcggt cgtcaggtcg agtgtgtaga tctcgtaatc
     1981 accgccggcc ctgttgctcg cgaacacgag cgtgcggccg ttcggagaga tctccgggtg
     2041 gcgtccttcg ccgaacgaac ggacggtctt ggacgggagc tcgacgagac gcacctgcca
     2101 ctgtccggca gggtcgtcgg gctgcacgtg gtaggcgatc gcactcgtgc cgttggcaag
     2161 ccggccccat tccgcgtagc cctcgaggtc gggcgtatcg atcagcaccg acggcgacgc
     2221 cgtcggaggc gacgcggtga cgatgtcggg atcacccgtg ctcgtcgacg acacccatgc
     2281 gagcgacgag ccgtccggcg accatgcagg tgtcacgtcg aacgcgtccg gcgtcccgtt
     2341 gaggaacgtc gcggcgcccc cggtgctcgg cacctggtag atctggaagt cgggtgggct
     2401 gccgccggaa ggcgatccga tcatgcgctg gaaggccacc gtgcgcccgt cggggctcaa
     2461 gtcgggcgat ccctccgacg ccgggtccga cgtcaggttc gccttgtccg tcccgtcgcc
     2521 gtccatgatc cagacgtcgt agtcgccgtc gactcccgac gtgtacgcga tctgcccgcc
     2581 ggcctgtccg ggcggaggcg tgggcgacga cgtcggggtc ggggtgggtg acggcgtcgc
     2641 cggtgggacg tcgtcgttgg tgatgatcgc gcggccctcg gcgttgccga tgagcgcgtt
     2701 cgtcacctgc tggatcttta tcgagaacga ctcgttggtc tccgagaccg cgtcggcgac
     2761 gacggggacc gtgaacgtct tcgtctgctc gccgggatcg aacgtgatcg tctggtcgac
     2821 cgcggtgtag tcggtgccgg tcgtcgcgga cccggccagt gtcgcgaccc gcaccgtgac
     2881 ctgctcgccc ggcgacaccg gcgcggacag gaacaccgcg aacgtgaagt tcgtcgtgcc
     2941 ggtggtctga cccgaagcgg gattgccctc ggccagagtc aggcgctgct gcaccgacgc
     3001 ggtcacctgc ggcgcccccc agtcgggctc gttctcgtcc gtgtcgttga acgtgagcct
     3061 gcggcgcgtc gccgtgccac cctcgggcgg ctgggtgtcg acgacgaaga tctcgaggtc
     3121 ctggtcgctg ggctggtcct tgctcgcgag cgcgatcttg cggtcgttcg gagaccacga
     3181 cggcgcgacg tcgttgaacg gggtgaacgt gaccttcacg tcgggcgtct cggcgggcgc
     3241 ggtcaggtcg aacacgtaga cgtcggtctg ggtgtccccc gcgagcctcg cgacgtaggc
     3301 gatcttgttg ccgctggggg agacgtcggg ctgcgtcacg cgcgtccccg ggtccgtcag
     3361 cgtgaggacc tcgccgccgg gggccgcgtc cgcgtcgtag gcgagctgcc cggcccgaga
     3421 ccacaggagg ccaccggtcg ggtgccacgt cgggtcgtcg tcttcctggt tgggcgtgtt
     3481 ggagacgttc gtgacgcgct tcgtgtcgag gttcacgacg aggacgtcgc cggtggtcgc
     3541 ctccttcccg cccctgaaga tctcggcgtc gaaggcgatc gagcgcccgt ccggcgacag
     3601 cttcggatcc cctacgtccg tcgcggacgg ctcggtgtgc gtgtagacct gctcggcccc
     3661 cgtgccgtcc gggttcgaca tgcagaacgt cagcacgtga cagcgataga cgaccttctt
     3721 cgcgtcgaac gaccagctcg gctcgcggtc ggcctggacg ttcttcgtca ggcgcctcgg
     3781 ctggtacccg ttcgtgccca tcgtgtagat ctccgggcgg atcgggccct gctggaagac
     3841 gatccgctcc ttgagctcgg acgtcggggg ctcagtgatc gtgatctcct gcgacatgtc
     3901 tgcgatcggc tcgatcgccg cggggtcgtc ctgcatcgag gtggggaaca cctcgcaggc
     3961 agtgaggagc atccccgcgc acgcgaaggc gagcagattt ctgcccttca tctacttcct
     4021 cctcacgacg accttgccgg ccgcgcggac gccactgatc tcgtgcagca gagacgcgta
     4081 gccgaagccc atcttcggcg acggcttcga gaacaacagg tcgtaggggc gagcgtcggt
     4141 gacgagcccc gccccgcgca gccccgagct cgcgatgtcc ttcgcgctcg tcagccgtgc
     4201 gtcgcccgcg ggggacgcga gcccggccgg gacctcgagc tcgagctccg cgtagctcgc
     4261 gcagtacgga gcctgagccg acgtgggcgc ggtgtcgggg gcctcggact cgacgtctcc
     4321 gtccgggtcg caggcgatca ccgggaaggc gttcgcctgc gagatcgcgc gccccgcggg
     4381 gaacgtgacg gtgtaggcgc tgaagacgtt cttgttgaag ttccagcgaa cgccctggcc
     4441 cttcttgatc gtgatcttcc tcgggtacat cccgcgcaac gagaccgtct gcgtctcgac
     4501 ccccgcccac gccatccgat acgtcttgcc cctgaccttc ttcgtggcgt tccggcgcga
     4561 gtaggagcgg tgcagacggt tggcggtcgc gacgtcctgc gcgaagacct cgtcggcctg
     4621 ctcggcgagc gtcgccgggt ccgagacggg ctcgtcttcg ccgacgacct cgatccgcat
     4681 cgacatcttc gggttgacga cgtcgacgac ctccacggcc tcgcccgggt cggcgttgat
     4741 cgtgaccgag aagtcgagct gatgcgcctg cgcggagtcg ccctgcggga cgagcgcgag
     4801 gcccgacccg agcacgtcgc ccagctcgtg cggccgggcg tcgaactcgc acggcgcctc
     4861 ggtcggccac ccgcagtcgc ggcttgggaa gatcgccgag aggttgccct tcagcacgtc
     4921 ggggccctcg tccgcgtccg gctcgaacag tgaccaggga cgcccgaccc cgccggcgtt
     4981 cgcggcggcc cactcgcccg ccgtctctcc gagcggcagg agcgcgacac cgtggaagcc
     5041 cacgctcgtg aagtgaacga cgtcgccctg gcgcacctgc agctcgctcg gatagaagcg
     5101 catcgactcc ttggtgaggt ctccctcacg gatgaggcgg ccgacctcga cctcgaagac
     5161 cgactgggcg cgcgcctcta ccaggggcag ggcggcgacc ccgagcatcg agacgaggag
     5221 ggcggcggcg acgcgccacc ggagcgcagt tgcaaaatgc atgaagggcg attctctccg
     5281 agggcgtgcg cgtatctcac ccggaatgac tacgtagttc acgcaagttt ttcttcgcgg
     5341 gctggctcgc gtcggttact cggaacccca gatcgagatc gtgtcgacgg cgcggaaccc
     5401 gagacgtgcg tacatcggca cggccttggg ggccgacgtg agccacaccc atccggcccc
     5461 cgccgcgaaa ccctcgagcg cggcgcgcgc ggtcacggcg gcgccgtagc cgcggccgcg
     5521 atgcgccgcg agcgtcgcga ccgacacgat ccccgtcgcg ccccggcagg tcacgccgac
     5581 cgccgtcgtc acggcctccc cgtcgacgcg tcccacgtag cacccgatct cggtcacggc
     5641 gagcagggcc ggggttgccg ccgcttcgaa cgcggcgacc ggggcctcga acgcggtggc
     5701 ggccacgcga gcgtggtcgg cgccctcgcc gggcgcgagc cggtcgactg cgagccccgg
     5761 cacctccgcg gcgcgcctca acgcggtgtc ctcctcgagg atcatcaggg gctcctcgtc
     5821 gtcgaagacc atcccgcgtc gccgcggtat ctctctgagc gcgggagccg ccccgctcgg
     5881 gagatggagg gagtggagga ctgccgcgga cgcagcccgg tccaacaggc cgtcgacgac
     5941 ctcggccggg acgtcaccgc gctccagcca cacgccgttg aacccggtcg tgtcgatgcc
     6001 ggtcagcacg agagcggccc cgtcccgctc ctccagggat gcgcccggga tcgcgtccac
     6061 gtacaggcgc cacatgtcga tcgagtgacg ggccgcggcg gcagcggtgg gcggaagacg
     6121 caccggtgga gtgtcgcgtg gccttgcttg acctgctatc gacgggactc ccataatcgg
     6181 caatccggcg ggtgctttgg acggtggcgg gaggcgcggt ggctcaatcc agcggttgcg
     6241 agagtttcca ggtaggcacg cgcaagtgga ccgattgcgt cgagggccag gcgaccggcg
     6301 gcggtctgat gccgtggatc gtggtcatcc cgctgggggt gatggtcctc gggatgatgg
     6361 tcgggttcgc gcgccagttc tcgggggccg gacagcggcg ggcgaaggca cacggggccg
     6421 ccggcactgc cgggacgtgg ctgatcttcg tttcgttcgt cgagctcgcg atcggattgg
     6481 gatcgctcga agccgagcgc agagcggcgg gatccggcgg cggcttctcc atggcggcga
     6541 cggtcctcct cggcgtcggc gcgctgttgt tcgtgatcgg cgtctacctc aagatccgcg
     6601 ggcggcgacg ggcgcgcatc tacaactccg gcgtcccggg cgaggccgtg atccgtgcgg
     6661 tccaccagac cgggaccatg gtgaacaacc agccgatgta cgccttcgac gtcgaggtga
     6721 cgggccaggg cttcgctccc gtgagcaccc gcgtccgcga ggtcgtgccg ttctggttcc
     6781 tgaaccgggt ggggccgcag gcgcgcgtgc cggtgaaggt cgacccccag aacccgacgc
     6841 gcatgatctt cgactgggac gcgatgggga cgatggcgac acaggtcggg acgccggccc
     6901 cggcgccgat ggcatcgtcg ttcggtggtg tccagagcgg gcaggtgacg accgccggag
     6961 gggtcggaca gggcgcgcag cagatgatgc cgagcgcgga ctccctcgcc agcgcgatgc
     7021 aagctgcgcg cgaggccacg gcgaacgcgg gctccggctg gcacgtcggg aaggtcatcg
     7081 gcctggcggt cacgttgttc gtcctcgtcg tgatcggcgg cggcctcttc ttcatcgcgc
     7141 gcatcttcgg ccaggtgagt gacgtgacgg acagcgtgac ggaccaggtg tccgacgcgc
     7201 tcgaccaggc ggacggtggg ttcggcgagg ggttccaggg gggcggtagt gccgcgacga
     7261 cgatcgaggt cacccggccc gcagccgggg gccggccggt cacgttccag gcgacgatcc
     7321 ccgtcggctg ggtcgacgtc acgccaacgg ggagcggtga gcggcagggg ccggtcctgg
     7381 tcgacctcgt ggccaaaccg caggcgccgt cggaagcgcg catcgtcgtg acgcgatccg
     7441 ttcgctacgt ggagaacccc gcgccacccg gcacggggat cgagagcatc cgctccgacg
     7501 tcgagaagga gttcggcgac agcctgacgg gcagcagctc cgtgacgctc gccggcgaga
     7561 aggcggtcct gctcgagatc gccccgggcg cggacggtct gcggtcgcgc cagatcgcgg
     7621 tgatgcgcgg cggccaggtc ctgttcgtgg ggctggtcgc gccgaagtcc gaatgggggt
     7681 cgttcgtgtc cgtcttcgac gacgtcgtcg agtcgtggaa gtggcgcaag gtcacgacgt
     7741 agcgggtcac cgtcgaggag ccggggatgg tgccggagtg ggcggatgcg gtcttcgtgc
     7801 tgtgcggcac gatcgtttgc gtagtggcgt tccgctacgg cgcggccgcg cggccctcgc
     7861 ccgagcgagc gctcgccgcg gcgaggatcg gcaagctcgt catgagcgcg ggtggggtgg
     7921 ccgcgtcgat cgcgacggcg tcagtgttcg ccgacggatc gatcccgacg atcggctggg
     7981 cgatgttcgg gctggcgggg atagcgctgg gctccggggt cgcgctgcgg ctgggggccg
     8041 ggtggccccg gacacgaccg taaggaccgg aacgtcgcgg cgactagctc cgtccgatgt
     8101 cgggctcggc gaaagcgaac gcggtaccgg tacgccatgc ctcgaactcg gtgtgaggct
     8161 cgccgaggtg ccgtacgagc ggaaggagct ccgccggtgc tctcgagccc tcggcgcaaa
     8221 tcgaggtggg gacgtcggtt gggcctatcg ggtcgatcaa gtacggaacc gcgacgcgag
     8281 gtccgaacat caccaggttg cgtgtgcccg gtagagctgc gctcggcact tccaacacct
     8341 ccggcgagtc accttcgcga agacaccggt cgccaagctc ttgacacgcg ccgtagtcat
     8401 cggagatgag gtgttgcggc tcgagtccgt aatcgccggc gttgtcgaaa ttgacttcct
     8461 cgaccttcct caccttcacg cgcgacaccc agacgcgaag acgcaactcc tggagcgcgt
     8521 ccggcgaacg gagtccttga gcgcgcagca cctcagcgag tggtcccaag gggtgcaggg
     8581 cccagtattg aacgggtccc ctgcccgcct tgttgaacct ccgcggccac ctattcgggc
     8641 tgacccagaa gggcgtatcc cagccggcga tgcggtaggc gacgatgtcg gccatccggg
     8701 ttgctacgta gacgcgctgg agcgcgccgc cgcagcaagg tgaaccagct gttcgaagtt
     8761 ctcgcgtttc gacagaaggt ctcgcggcgc agcgcccttg agctctggat gtgggcgctc
     8821 cagccaggcg agcacgccgg gaccactaaa ggcgtggcga aggttctgtg cgacgcgcgc
     8881 caacacccgg accttcaacg cctcctcgtc ccgaggttct gagggatccg atgacgagat
     8941 ccatcgctgg aacgttcgac cgttgaccga cagggctccg gcaatctcgg cctgcggtac
     9001 cgccagcgct tcgactaacc accgagcgac gtccttcgag gaccggtctt cgctcgtggg
     9061 gatgccctcg atgatgtccc gcgcggcctg gcgcatcttc tccagcgcga ctctgagcat
     9121 cgtccgctta cgcgacgagc tgttgacgtc caatgcgaac agggcgatga gcgccccttg
     9181 atgcaatgcc aggaggaggt atggatcgac tgcatccgac tcctgcggcg agatgttcat
     9241 cacgtccgag ccgatcttgc gaacgagctt ctcgacccgt tcggggacct ctttctccct
     9301 ggcaagcgtc agattcgcgt cccgcaactg tcgctcgagc gcggccgcgg ccgtgcggtc
     9361 gagcacggag tttgccaacg gcctcatcct tcccattgac gtcttctcgt catattagta
     9421 cgacaatttt aggacactcg gcggattcca cctaggtcgt cgcccgcgag gggcgggggg
     9481 gtgccgcact ccggcatgtg cggctcggcc gcgggcggtg cccaccactc gggcgtgacc
     9541 tcgtccacgt ccggcgtctc cgtcaggttc acgacgtcgc tgccgtcggg gctcacggtg
     9601 tagaggtcgg acttgcccgc gggggccgcg tcgaacagga tcctcgagcc gtccggcgtc
     9661 cacgagctcc cgctcgcccc cggggccttg tggaccagcc tgggctcgcc tccgtcgacc
     9721 gggagcgccc ttcgccgagt gtgggcgcgt cgcgacgatc ggtccgctca cttctgaaca
     9781 cccgcgcgc
//
LOCUS       DASQDO010000069         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_130541, whole genome shotgun sequence.
ACCESSION   DASQDO010000069 DASQDO010000000
VERSION     DASQDO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130541"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VEV43_02205"
     CDS             <1..734
                     /locus_tag="VEV43_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22360.1"
                     /translation="ELPHAHEELTPSRLTVVEPADLYALPLEEFTSARNDLARALKDE
                     GRAEEAARVKKLKKPPVSAWVVNQLARDDSDGIARLLDLLDRLKEARSAPDIQKLGAQ
                     RRELVAKLTRRAGELAEAAGHAASATTLQRVTQTLQAGAEDEERELLRRGALPDDLEP
                     AGFDALGAFSLDAFTSTPEPDDADDELEELKRLAAEAEEEAARLSAEAERAEAAALAA
                     SEAASEARRRAREAREKAQKAARSR"
     gene            complement(739..1110)
                     /locus_tag="VEV43_02210"
     CDS             complement(739..1110)
                     /locus_tag="VEV43_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYP22361.1"
                     /translation="MEDDKARQLLNDERTRLEGLRKRIDDPGVETQEDSLSELTTADQ
                     HPADVGSETFERTKNLSLQEDVDGRLADVDRALEKLDAGTFGTCETCGNPIPDERLEA
                     VPGARYCVEHQAQQERTVPAE"
     gene            complement(1157..1888)
                     /locus_tag="VEV43_02215"
     CDS             complement(1157..1888)
                     /locus_tag="VEV43_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYP22362.1"
                     /translation="MIRIAAAGDVHFGEDSAGTLQPHLPEVADRADLLVLAGDLTRCG
                     SPDEAAVLAKELSGLPLPVFTVLGNHDYHDGKQGEITQVLEEHGIRVLEGTGEVVTVG
                     GKRVGVAGIKGFGGGFAGACGSEFGEPEMKSFIRHTKQTSARLEEALGGLDADVKVCV
                     LHYSPVEETLRGERLEIYPFLGSYLLAEAVDRAGADIVFHGHAHAGSEKGVTPGGVRV
                     RNVAQPVIGKAFKAYCLGDGGHDDC"
     gene            complement(1885..>2095)
                     /locus_tag="VEV43_02220"
     CDS             complement(1885..>2095)
                     /locus_tag="VEV43_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017682259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYP22363.1"
                     /translation="ALATDPRVSTLGIEVSIRGDAVFLSGDVGTGERREAVAQVVAEL
                     LPGYTVHNDTSAPEFPAGVETEHLS"
BASE COUNT          299 a          755 c          741 g          300 t
ORIGIN      
        1 cggagctccc tcacgcccac gaggagctga caccttctag gctgacggtc gtggaaccgg
       61 ccgacctgta cgcgctgccg ctcgaagagt tcacgtccgc gcggaacgac ctcgcacggg
      121 cgctgaagga cgagggccgc gccgaggaag ccgcacgggt gaagaagctg aagaagccgc
      181 cggtctccgc atgggtcgtc aaccagctcg cgcgcgacga ttccgacggg atagcccggc
      241 tgctcgacct cctcgaccgc ttgaaggagg cgcgctcggc ccccgacata cagaagctcg
      301 gggcgcagcg gcgcgagctc gtagcgaagc tcacgcgccg cgcgggtgag ctcgccgaag
      361 cggccggtca tgcggcgtcg gcgacgacgc tccaacgcgt gacgcagacg ctccaggcgg
      421 gggccgagga cgaggagcgc gagctgctac gacgcggcgc cctgccggac gacctcgagc
      481 ccgcggggtt cgatgccctc ggcgcgttct cgctcgacgc gttcacgtcc acccccgagc
      541 ccgacgacgc cgatgacgag ctcgaagagc tgaagcgact ggccgccgag gcagaagagg
      601 aagccgcgcg gctgagcgcg gaagcggagc gcgcggaagc cgcggcgctc gccgcatcgg
      661 aggccgcgtc ggaagcccgc cgccgcgcgc gcgaggcgcg cgagaaggcg cagaaagcag
      721 cgcgctcgag gtagccggct actcggccgg gaccgtgcgc tcctgctggg cctggtgctc
      781 cacgcagtaa cgcgctcccg ggaccgcttc gagccgctcg tcggggatcg ggttcccgca
      841 cgtctcgcac gtgccgaacg tgcccgcgtc gagcttctcg agggcccggt cgacgtccgc
      901 aaggcggccg tcgacgtctt cttgcagcga caggttcttg gtgcgctcga acgtctcgga
      961 cccgacgtcc gcggggtgct gatcggccgt ggtgagctcg gagaggctgt cctcctgcgt
     1021 ctcgaccccc gggtcgtcga tccgcttacg cagcccttcg agccgcgtgc gttcgtcatt
     1081 caacagctgg cgtgccttgt cgtcttccat gggtgcgggg ttacccaacg cccgccgcgc
     1141 taaccgtcgc gcggctctag cagtcgtcgt gcccgccgtc acccaggcaa taggccttga
     1201 aggcctttcc gatcacgggc tgcgcgacgt tgcgcacgcg caccccgccc ggcgtcacgc
     1261 ccttctccga acccgcgtgc gcgtggccgt ggaagacgat gtcggcgccg gcgcggtcga
     1321 ccgcctccgc gaggaggtag ctcccgagga acgggtagat ctcgaggcgt tcgccgcgca
     1381 gggtctcttc gacgggggag tagtgcagca cgcagacctt gacgtccgcg tcgaggcctc
     1441 cgagagcctc ctcgaggcgc gccgaggtct gcttcgtgtg ccggatgaag ctcttcatct
     1501 cgggctcacc gaactcgctt ccgcacgcgc cggcgaagcc cccgccgaac cccttgatcc
     1561 ccgccacgcc gacgcgcttg ccgccgacgg tcacgacctc gcctgtgccc tcgaggaccc
     1621 tgatcccgtg ctcctcgagc acctgcgtga tctcgccctg cttgccgtcg tggtagtcgt
     1681 ggttgccgag gacggtgaag acgggcagcg ggaggccgct cagctccttc gcgaggacgg
     1741 ccgcctcgtc ggggctgccg cagcgcgtga ggtcaccggc gagcacgagg aggtcggcgc
     1801 ggtccgccac ctccggcagg tgcggctgca gcgtcccggc ggagtcctcg ccgaaatgga
     1861 cgtctcccgc ggcggctatc cggatcacga caggtgctcc gtctcgacgc cggcggggaa
     1921 ctccggcgcg ctggtgtcgt tgtggaccgt gtagccgggc agcagctccg cgacgacctg
     1981 ggccaccgcc tccctgcgct cgcccgtgcc gacgtcgccg ctcaggaaga ccgcgtcgcc
     2041 gcggatggac acctcgatgc cgagagtaga gacgcgcggg tcggtcgcga gcgcc
//
LOCUS       DASQDO010000070         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_864624, whole genome shotgun sequence.
ACCESSION   DASQDO010000070 DASQDO010000000
VERSION     DASQDO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864624"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VEV43_02225"
     CDS             complement(<1..978)
                     /locus_tag="VEV43_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22364.1"
                     /translation="MSVDVVLERYVAELDAAYKAARGTEPFDDSKILDLVEELVVACT
                     SALLDELGEPRPANESPDELVARATESIRNHPEAFVEDASVKSLERELHGLHDVLRDL
                     AYFRQGIGVLKNGHKPADMRWLTEGPMESYAVSALSRVSEALRSDLPQVHRIAPEAEV
                     DLAHTRAGIIALLRQDLGPDEFMSAAIDLGASQTGPAGWDAFRALAYEEELEKRSSFF
                     VDVISRNPPGAPLAGLYGEIAYPSRGGKTVADVDVTGSDAYEPDDEEWFGSITYTPRD
                     SLAGSEVLAKIYEMAYLPGGLGNAADYTLCLAWGAYLSRACARRFVLETG"
     gene            complement(1283..2473)
                     /locus_tag="VEV43_02230"
     CDS             complement(1283..2473)
                     /locus_tag="VEV43_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22365.1"
                     /translation="MKTSLRRLAVPLLAGVLLLGACSDGGGGEEEAAENPKQAFTEAL
                     DALAEYEGVTLELSLEADPAALEEDDTPPEVAEAIVNSSVTISAKGATLEDSQVQFTI
                     NVDGNEDAAELRSVDQAVYLRADVGELVEKFGGDPAQIDAFAQQATAQGFDFAQAFVD
                     GDWVGIEGLDDLAQQFGLPQPTADPEQAAEFVDQIAGILEQNVEVTSEGTDDVGAHLL
                     LSVPLRDTVEDLYNSLQEFVEAPAGTLPTDTLSDVPDVDVPVDVWVSDGRLVQVELDL
                     VAIAEAAGEEPPEDVDEFVVRVGVDEFTEDVEAPSDFAEIDLKQLFEAFFGASLGGMD
                     SAGGSAGSPPVPGGNREVVVPELGLACSDIQMLSPEEIEQFLSASGVPGALKQVRRAC
                     PELF"
     gene            complement(2519..3862)
                     /locus_tag="VEV43_02235"
     CDS             complement(2519..3862)
                     /locus_tag="VEV43_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22366.1"
                     /translation="MSRLLRVLAVVCVLVVLPVQPAKACSCAYGDPRDRFADADGAFV
                     GTFVESHPVEPNPSNSGADTIYTFLLEEEYKGELGDPGDTVEVHAPLSGASCGIEAAP
                     GQRYGLFLDVRDDGAWSSNLCSQVSPGTMREAASPLPAPTSDGPVRFVAGGSFGDTQT
                     MLLDARGRTVGYGAGDSDVVYVSGCRGRGRMIEVGIVYPGPAVLVVRDVSSQNTVRSV
                     ELPIGGGPKRVSGALCLSKSGRRAIVFATDHEGRAVLLEVTGTDVDVLWRGDARGAAF
                     AGRRAYIEKDGALGRVSLRTGAERRVTSLPGDYTSELAVSPDGTKLAGVAYPAYDRSD
                     ERPSQVFVVDLTRRRIRTASLGTGERDAHVRWLSDKRLAMFVLYPDASRVYDMKLEVR
                     SRFGRWQGHTTAIVGDTAYGVDYEGGLWSVDLPDGTPERVRRLPSPVVYDLAPLE"
     gene            3939..>4859
                     /locus_tag="VEV43_02240"
     CDS             3939..>4859
                     /locus_tag="VEV43_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP22367.1"
                     /translation="MTKPERRLYTLVVLAVALVLGMSTWFSASAVVPQLREEWGLGDS
                     AAAWLTIAVQLGFVAGAVLSSLLNLSDVLPVRSIMIVSSLGAAAVNLFLLVADGAATA
                     IPARFLTGAFLAGLYPPALKLMATWFTKGRGTALGVMVGALTLGSALPHLVNALGGLE
                     WELVVAVTSITTAAGAVIVWALVREGPYPFPTATFDVRQARLVFTDRRLRLTSAGYFG
                     DMWELYAMWAWFVVFFRSSLAEGGTVDGAGAAAATFAVIGIGGLGCYAGGVAADRWGR
                     ARLNLWSLAVSGTCAAVIGLTYGGPSWLVLA"
BASE COUNT          698 a         1717 c         1654 g          790 t
ORIGIN      
        1 ccccgtctcc agcacgaacc ggcgcgcaca ggcgcgtgag aggtaggccc cccacgccag
       61 acacaacgtg taatcggcgg cgtttccgag tccacccggc agataggcca tctcgtagat
      121 ctttgccagc acctcggacc cggccaagct gtcacgaggc gtgtaggtga tcgagccgaa
      181 ccattcttcg tcgtccggct cgtatgcgtc ggaacccgtg acgtcgacgt cggcaaccgt
      241 cttgccgccg cggctcggat acgcgatctc gccgtagaga cccgccaggg gagctcccgg
      301 aggattgcgc gagatcacgt cgacgaagaa cgacgaacgc ttctcgagct cctcctcgta
      361 tgcaagggcc cgaaacgcgt cccacccggc gggccccgtc tgcgacgcgc cgaggtcgat
      421 ggctgcgctc atgaattcgt ccggtccgag gtcttgccgt aggagcgcga tgatgccggc
      481 gcgggtatgg gcgaggtcga cttcggcttc cggagcgatc cggtgaacct gtgggagatc
      541 gctcctgagc gcctccgaga ccctgctcag cgcgctgacg gcatacgact ccatcgggcc
      601 ctcggtgagc cacctcatgt cggcaggctt gtggccgttc ttcaacaccc cgataccctg
      661 gcggaagtag gcgaggtcac gcagcacgtc gtggagtccg tggagctctc gctcaaggga
      721 tttgaccgag gcgtcttcca cgaatgcctc aggatggttg cgaatcgatt cagtggcgcg
      781 tgccacgagc tcatccggcg actcgttcgc cggtctgggc tcgccaagct cgtcgaggag
      841 cgcagaggta caagcgacga ccagctcctc aacgagatcc agaattttag agtcgtcgaa
      901 gggctcggtg cctcgcgccg ccttgtaagc cgcgtcgagt tcggctacgt accgctcgag
      961 aacgacgtcg acgctcaatc tcggtcgctc ccctgcacta gcacctccac cattcccaac
     1021 gaaccctggt cggatcgttc tcgatcactt cgagaaccca cgtcctccac cacgcctcga
     1081 acgattccca gtcggttgcc tcacgtaacg acggagcttc ggcgacttca gggtgcactc
     1141 gcgggcggga tgaacgtgct cgactggagc gatcccgacg cgcccgagga ggtcgcgcac
     1201 ttccgtacgg cgcggacgag ctattggtcc gcgtactggt accgcggcct cgacgtcttc
     1261 gaggtgcgcc ggctctcgcg cgtcagaaca gctcggggca ggcccgccgc acctgcttca
     1321 gagcacccgg gacgccggac gccgagagga actgctcgat ctcctccggc gacagcatct
     1381 ggatgtccga gcacgcgagg cctagctccg gcacgacgac ctcgcggttg ccgcccggca
     1441 ccggcggaga cccggccgag ccgccggcgg aatccatccc gcccagcgac gcgccgaaga
     1501 acgcctcgaa gagctgcttc aggtcgatct cggcgaagtc cgacggtgcc tcgacgtcct
     1561 cggtgaactc gtccactccg acgcgcacca cgaactcgtc gacgtcctcg ggcggctcct
     1621 cgccggcggc ctcggcgatc gccacgaggt cgagctcgac ctggacgagg cggccgtcgc
     1681 tcacccagac gtcgacgggc acgtctacgt ccggcacgtc ggagagggtg tcggtcggaa
     1741 gcgtccccgc aggtgcctcc acgaactcct gcaacgagtt gtacagatct tcgaccgtgt
     1801 ctctcagcgg gacgctgagc aggaggtgcg cgccgacgtc gtcggtgccc tcggacgtca
     1861 cctcgacgtt ctgctcgagg atccctgcga tctggtcgac gaactcggcc gcctgctcgg
     1921 ggtcggcggt gggctgagga aggccgaact gctgggcgag atcgtccagc ccctcgatcc
     1981 cgacccagtc accgtcgacg aaggcctgcg cgaagtcgaa cccctgcgcg gtcgcctgtt
     2041 gcgcgaacgc gtcgatctgc gcggggtcgc ccccgaactt ctcgacgagc tcccctacgt
     2101 cggctcggag atagacggcc tggtcgaccg aacggagctc ggcggcgtcc tcgttcccgt
     2161 ccacgttgat cgtgaactgg acctgtgagt cctcgagggt cgcgcccttc gccgagatcg
     2221 tgaccgacga gttcactatc gcctcggcga cctccggcgg cgtgtcgtcc tcctcgaggg
     2281 ccgcggggtc cgcctcgaga ctcagctcga gcgtgacgcc ttcgtactcc gcgagcgcgt
     2341 cgagcgcctc ggtgaacgcc tgcttcgggt tctcggcagc ctcttcctcg cctccgccgc
     2401 cgtccgagca ggcacccaac aacagaacgc ctgcgagcag cggcaccgca agccgcctga
     2461 ggctcgtctt catgtggatc ctccttgcgt ctgggtcacc gccacggtac ctcggcggct
     2521 actccagcgg cgcgaggtcg tacacgaccg ggctcggcag ccggcgcacg cgctcgggcg
     2581 tcccgtcggg caggtcgacg ctccacaaac cgccctcgta gtcgacgccg taggcggtgt
     2641 cgccgacgat cgcggtggtg tggccctgcc agcggccgaa ccgggagcgc acctcgagct
     2701 tcatgtcgta gacgcggctc gcgtccgggt agaggacgaa catcgcgagc ctcttgtcgg
     2761 agagccagcg cacgtgggcg tcgcgctcgc ccgtgccgag cgacgccgtc cggatgcgac
     2821 ggcgggtcag gtcgacgacg aagacctgcg aaggccgctc gtcgctgcgg tcgtacgccg
     2881 ggtacgcgac gccggcgagc ttcgttccgt ccgggctcac cgcgagctcg ctcgtgtagt
     2941 cgccgggcag cgaggtcacc cggcgctcgg cccccgtgcg cagcgacacg cgcccgagcg
     3001 cgccgtcttt ctcgatgtac gcgcgccggc cggcgaacgc agctccccgc gcgtcgcctc
     3061 gccacaggac gtcgacgtcg gtgccggtga cctcgagcaa gaccgcgcgc ccctcgtggt
     3121 cggtcgcgaa cacgatggcc ctccggccgc tcttcgacag acagagcgcg cccgagaccc
     3181 tcttggggcc cccgccgatc gggagctcga ccgaccggac ggtgttctgc gaggagacgt
     3241 cgcgcacgac gaggaccgcc gggccggggt acacgattcc gacctcgatc atcctgccgc
     3301 ggccgcggca ccccgagacg tagacgacgt cggagtcgcc ggcgccgtaa cccaccgtgc
     3361 gcccgcgtgc gtcgagcagc atcgtctggg tgtcgccgaa gctcccgccg gcgacgaacc
     3421 ggaccgggcc gtcgctcgtg ggggccggaa gcggcgacgc ggcttcacgc atcgtcccgg
     3481 gcgagacctg gctgcacagg ttcgaggacc aggcgccgtc gtcgcggacg tcgaggaaca
     3541 atccgtacct ttgacccggt gccgcctcga tcccgcagct cgcccccgag agcggggcgt
     3601 ggacctcgac ggtgtcaccg ggatcgccga gctcgccctt gtactcctcc tcgagcagga
     3661 acgtgtagat ggtgtcggcc cccgagttcg acgggttcgg ctcgacgggg tggctctcga
     3721 cgaaggtccc gacgaaggcg ccgtcggcgt cggcgaagcg gtcgcgcggg tccccgtagg
     3781 cgcacgagca cgccttggcg ggctggacgg ggagcacgac gaggacgcag acgaccgcca
     3841 ggacccgcaa cagcctcgac atgactcact ccttgcgctt gggggctctg ctcttagaga
     3901 cgaaaccacg ccgggaaagt tcccgccatg atgctcgggt gaccaaaccc gagcggcgcc
     3961 tgtacacgct ggtcgtgctc gccgtggccc tggtgctggg gatgtcgacg tggttctccg
     4021 cgtcggcggt cgtgccacag ctgcgggagg agtggggcct cggggactcg gccgccgcgt
     4081 ggctgacgat cgcggtgcag ctggggttcg tcgccggtgc cgtgctgtcg tcgctgctca
     4141 acctctcgga cgtcctgccg gtgcggtcga tcatgatcgt ctcgtcgctg ggggccgctg
     4201 ccgtcaacct cttcctgctc gtcgcggacg gcgccgcgac ggcgatcccc gcccgctttc
     4261 tgaccggtgc gttcctcgcc gggctgtacc cgccggcgct gaagctgatg gcgacgtggt
     4321 tcacgaaggg ccgcggcacg gccctcggag taatggtcgg cgcgctgacg ctcgggagcg
     4381 cgctccccca cctcgtcaac gcgctcgggg gtctcgagtg ggagctcgtc gtcgcggtga
     4441 cgtcgatcac gaccgcggcc ggcgcggtga tcgtctgggc gctcgtgcgc gagggcccgt
     4501 acccgttccc cacggcgacg ttcgacgtgc gccaggcgcg gctcgtgttc acggaccggc
     4561 gcctgcggct cacctccgcc ggctacttcg gcgacatgtg ggagctctac gcgatgtggg
     4621 cgtggttcgt cgtcttcttc cggtcgtcgc tcgcggaggg tgggacggtg gacggcgccg
     4681 gtgccgccgc cgcgacgttc gccgtcatcg gcatcggggg gctgggttgc tacgccggcg
     4741 gcgtcgcggc ggaccgttgg ggacgcgccc gcctgaacct ctggtcgctc gcggtgtccg
     4801 gcacgtgtgc tgccgtcatc ggtctcacct acggcgggcc gtcgtggctc gtgctcgcg
//
LOCUS       DASQDO010000071         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_681012, whole genome shotgun sequence.
ACCESSION   DASQDO010000071 DASQDO010000000
VERSION     DASQDO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681012"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VEV43_02245"
     CDS             complement(<1..583)
                     /locus_tag="VEV43_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22368.1"
                     /translation="MNVRRRALKILAVSLTLGFVALQAAPATAKPIKDEPPVLITAWY
                     WEDQERQRIQDPTSGTDVAVISVPNPFCPSAPVVGSPSPESCREGRLPIQVRDGDYET
                     PNQLSAIGFDLALVPPGSKIGKFTVTLVEAKDDQSRPVNAEGKSLQACFIEEFFGDGE
                     AALYKEVPKYTCSEGDPIAPRKELKVKEEDGGGN"
     gene            721..1314
                     /locus_tag="VEV43_02250"
     CDS             721..1314
                     /locus_tag="VEV43_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22369.1"
                     /translation="MKKLRSAWQSLWVDVDRRVHVGRILGLVFVTAGFVVIGKAWDGA
                     ASINFTTGQFPYLLSGGFMGLGLIVTGCTLLLLATVRGEREVMSQKFDEVAKLLSRSL
                     TRMQSPSNGVAGSGEQVVAGETTYHRADCKILQGKTGLSTLTVEQATAEGLTACRVCD
                     PPQLAAKAEEKAEEKSTANGDGAAPAEEPAEETSASS"
     gene            complement(1311..2564)
                     /locus_tag="VEV43_02255"
     CDS             complement(1311..2564)
                     /locus_tag="VEV43_02255"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HYP22370.1"
                     /translation="MPELRPFHGIRYSSSSDLAKLICPPYDIISPDEQAGLHALHPHN
                     AVHLELAQGGRSSYDAAGETFREWLADGTLARDPQPSLYVYRQDFVDAAGTRRRVVGV
                     TGALALEPFGDSAGVLPHERTMAGPKEDRLALMRAVRANVSPIYAVYRGAGGLGPFYD
                     SLENRPTAGRAQDGHGILHRLWVISAPGEVAMLQDAIAPGPLVIADGHHRYETALAYH
                     AERQGEPRPIGSAIGQGKNDHDSIMCFCVDADSEELVVLPYNRVLRADVTSDELAGRL
                     RENLGAEQLTGDPQEFLASSKADHAFAFVLDGARLGVEVSDAQVVDAVGDRAAAWRAL
                     DVVALHEVVLPAVLPGGSAELAFTKEPAEIDRRVAEGWTAGVLLRALRPADVVEVATS
                     GERMPQKASYFWPKAATGLVFHELG"
     gene            2571..3359
                     /locus_tag="VEV43_02260"
     CDS             2571..3359
                     /locus_tag="VEV43_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HYP22371.1"
                     /translation="MIDERLDALDGLVCDMDGVVYRGPEPIEGVAEGIARWRGRGKRL
                     VFCTNNSHFTVPEYVSKLAAVGIPADEGDVVSSGAVLTEVLRERRAEGKKAMAVGAQG
                     LRSGLEAAGVTIDDGDSVEGLDFVVVGWDGEFDYRKMKRACLAVRAGASLLATNSDAT
                     FPAPDGLWPGAGSILASIEVSSGVRAEVLGKPHAPMMEAAARRLEGCTRIGIAGDRAD
                     TDLAGGLSRGWTTILVTSGVTTPEEAEQIEPRPDVILPSLASLP"
BASE COUNT          570 a         1298 c         1430 g          604 t
ORIGIN      
        1 cgttgccgcc gccgtcctcc tccttcacct tcagctcctt gcggggggcg atcgggtccc
       61 cctcgctgca ggtgtacttc gggacctcct tgtagagggc cgcctcgccg tcgccgaaga
      121 actcttcgat gaagcaggcc tggagcgact tcccctcggc gttgacgggc cgggactggt
      181 cgtccttcgc ctcgacgagc gtcacggtga acttgccgat cttggagccg ggcggcacga
      241 gggcgaggtc gaagccgatc gccgagagct ggttcggcgt ctcgtagtcg ccgtcgcgga
      301 cctggatcgg caggcggcct tcgcggcacg actccggcga cggcgaaccg acgacgggtg
      361 cgctggggca gaacgggttg gggacggaga tgacggcgac gtcggtccca ctcgtcgggt
      421 cctggatgcg ctgcctctcc tggtcctccc agtaccacgc ggtgatgagg accggaggct
      481 cgtccttgat cggcttggcc gtcgccgggg cggcctggag cgcgacgaaa cccagcgtca
      541 acgagaccgc gaggatcttc agagcgcgtc ttcgcacgtt caccctccct gggacggtcc
      601 ggacctcctg agggttccca ccgtccctca cccgtgatgg ttatgcccgg tcggatgcgc
      661 ctaacactag caagtcaacg gaataagtgg ccaagaggtc gttgcgctac gctcggtcac
      721 gtgaagaagc tccgctccgc atggcagtcg ttgtgggtcg acgtcgaccg gcgagtccac
      781 gtgggccgga tcctcggtct cgtctttgtc acggcggggt tcgtcgtgat cggcaaggcg
      841 tgggacgggg cggcgagcat caacttcacc acgggccagt tcccgtacct gctctccggg
      901 ggcttcatgg ggctcggcct gatcgtgacc ggctgcacgc tcctgctcct cgccaccgtc
      961 cgcggcgagc gcgaggtcat gtcgcagaag ttcgacgagg tggcgaagct cctgagccgc
     1021 agcctcaccc gcatgcagtc gccgtcgaac ggcgtggcgg gttcgggcga gcaggtcgtc
     1081 gccggcgaga ccacctacca ccgggcggac tgcaagatcc tccagggcaa gaccgggctg
     1141 agcacgctca cggtcgagca ggccacggcc gaagggctga ccgcgtgccg ggtgtgcgac
     1201 ccgccccagc tcgcggcgaa ggccgaggag aaggccgagg agaagtccac ggccaacggc
     1261 gacggcgcgg cccccgcgga ggagccggcc gaggagacct cggcctcgag ctaaccgagc
     1321 tcgtggaaga cgaggcccgt ggcggccttt ggccagaagt agctcgcctt ctgaggcatc
     1381 cgctctcccg aggtcgcgac ctcgaccacg tccgccggcc gtagcgcgcg caacaggact
     1441 ccggccgtcc atccctcggc cacccggcgg tcgatctcgg cgggttcctt ggtgaacgcg
     1501 agctcggccg atccgccggg gagcaccgcc ggcagcacga cctcgtgcag ggcgacgacg
     1561 tccagcgccc gccaggctgc cgcccggtct ccgaccgcgt ccacgacctg ggcgtcggac
     1621 acctcgacgc cgagcctcgc gccgtcgagc acgaacgcga acgcatggtc ggccttcgag
     1681 ctcgcgagga actcctgggg gtcgccggtg agttgctccg cgccgaggtt ctctcgcagg
     1741 cggcccgcca gctcgtcgct cgtgacgtcc gctcgtagca cgcggttgta cgggaggacg
     1801 acgagctcct cggagtccgc gtcgacgcag aagcacatga tcgagtcgtg gtcgttcttt
     1861 ccttggccga tcgccgagcc gatcggtcgg ggctctcctt gccgttcggc gtggtaggcg
     1921 agcgcggtct cgtagcggtg gtggccgtcg gcgatgacga gggggccggg cgcaatcgcg
     1981 tcctgcagca tcgccacctc gccgggggcc gagatcaccc acaggcggtg gaggatcccg
     2041 tgaccgtcct gggcccgccc cgccgtcggc cggttctcca gcgagtcgta gaaggggccg
     2101 aggccgccgg ccccccggta gaccgcgtag atgggggaga cgttcgcccg caccgcacgc
     2161 atgagcgcga ggcggtcctc cttcggccca gccatcgtcc gctcgtgcgg gaggactccc
     2221 gcggagtcgc cgaacggctc gagcgctagc gcgccggtta ctcccacgac gcggcgccgg
     2281 gtaccggccg cgtcgacgaa gtcctgccgg tacacgtaga ggctcggctg cgggtcgcgg
     2341 gccagcgtgc cgtcggcgag ccactctctg aaggtctcgc cggcggcgtc gtagctcgac
     2401 cgtcccccct gcgcgagctc gaggtgcacc gcgttgtgcg ggtggagcgc gtggaggccg
     2461 gcctgctcgt cgggcgagat gatgtcgtac ggcgggcaga tcagcttcgc aagatccgac
     2521 gaggaggaat agcggatgcc gtggaacggc ctgagctcgg gcacggcgag atgatagatg
     2581 agcgcctcga cgcgctcgat gggctcgtct gcgacatgga cggggtcgta tatcgggggc
     2641 cggagccgat cgagggcgtg gccgagggca tcgcccgctg gcggggccgc gggaagcggc
     2701 tcgtcttctg cacgaacaac tcccacttca ccgtgccgga gtacgtctcg aagctcgcgg
     2761 cggtcgggat ccccgcggac gaaggcgacg tggtgtcgtc gggggcggtc ctgaccgagg
     2821 tgctgcgcga gcggagggcc gagggcaaga aggcgatggc cgtgggggcc caggggctgc
     2881 gctccgggct cgaggccgcg ggggtgacca tcgacgacgg tgactcggtc gagggcctcg
     2941 acttcgtcgt cgtcggctgg gacggcgagt tcgactaccg gaagatgaag agggcgtgcc
     3001 tcgcggtacg ggcaggggcg tcgctgctcg ccacgaactc ggacgcgacg ttcccggccc
     3061 ccgacgggct gtggccgggg gcgggttcga tcctggcttc gatcgaggtg tcgagcggcg
     3121 tgagggccga ggtcctgggc aagccgcacg cgccgatgat ggaggccgcg gcccggcgcc
     3181 tggaaggctg cacgcgcata ggcatagccg gcgaccgagc ggacacggac ctggccggcg
     3241 gcctctcccg cggctggacg acgatcctgg tgacgtcggg cgtgacgacg cccgaggaag
     3301 cggagcaaat agagccgagg ccggacgtga tcctgccgag cctggcctcc ctgccgtagc
     3361 cccgaaccgg tcttggcgcc ctcgtggcga cctcgctgac gctgctgggc gtactttgcg
     3421 acgagagcct ggctttcatg gcggggcttt ctacgctgaa ggttgtggac atccttttcg
     3481 gatactcccc gggagattga ggacggcatg ggcaaggcga aggatttctg gcgagagatg
     3541 gtcccgatcc tcgggcgcac tcatcctgcc ttcaggacac gcgtccggcc ggcggagcga
     3601 ggcccggacc atgacaacgg cgaccttcag tccgaggtcc agggcttgcg cgagcaggtt
     3661 cgcgagctct ccgacgagct cgaccagcag aggaaggtgc tgtcgggtgt cgtcgccaag
     3721 gtcggcgggg aggatccgcc gcctgctcga tcggagccct aacgggtcag cgccggaaca
     3781 aacggtccag ctcgcgtagg cgcggctccg gcaggtcttc ggcgaagccc acgtgcaggg
     3841 cctcgtcccc gcactctttc tgctcgaact tcagcactta gtagtcgcgg cccccgtgcg
     3901 ag
//
LOCUS       DASQDO010000072         1785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_559002, whole genome shotgun sequence.
ACCESSION   DASQDO010000072 DASQDO010000000
VERSION     DASQDO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559002"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /gene="hemG"
                     /locus_tag="VEV43_02265"
     CDS             complement(<1..641)
                     /gene="hemG"
                     /locus_tag="VEV43_02265"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HYP22372.1"
                     /translation="MSGKVVAVVGGGITGLVAARELDRAGADVVVLEAADDAGGKIVT
                     KTLEGVAVEGGPDSFLVRDPWPVELCRAIGLGNDLIKPAVFGGLVWSRGRLHPLPRGL
                     VLGIPTLPVKMARGGLLSFRGSLRGVGDLFNSKPLTGKDVSVGALVRHRLGDEVLERL
                     VDPLLAGTRAGDADEMSLAAAAPELDTIARRHRSLVLGLRAWKPERPQTPVFMA"
     gene            complement(638..1564)
                     /gene="hemH"
                     /locus_tag="VEV43_02270"
     CDS             complement(638..1564)
                     /gene="hemH"
                     /locus_tag="VEV43_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HYP22373.1"
                     /translation="MVRSGVIVMAYGTPAGLDDVERYYTDIRHGRPPSPELLAELTAR
                     YEAIGGRSPLLEITEAQRIGLEKRLDGVPTYLGQKHSPPFIPDAVRAMADDGVDRIVG
                     LVLAPHYSKMSVGDYRRRVMLARDEAGLGAPVDVVESWHVEPGYVDFLAQRVREAAAR
                     LPDDARDRHVVVFTAHSLPEAILRGGDPYPEQLQETAGAVAAAAGVESYTVGWQSAGR
                     TADPWLGPDVLEVIERLAGEGARGVIVCPCGFVSDHLEVLYDVDVECVDAARRLGVEL
                     VRTESPNDDPAFLDVLATVVSRLLDKTGDAPG"
     gene            complement(1558..>1785)
                     /locus_tag="VEV43_02275"
     CDS             complement(1558..>1785)
                     /locus_tag="VEV43_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYP22374.1"
                     /translation="VPLDWARARLGDGVVLQGNLDPALCLAQFEVVRERAHEILDETG
                     GRRHVFNLGHGVLPSTDPDTLARLVEAVHEW"
BASE COUNT          250 a          674 c          627 g          234 t
ORIGIN      
        1 gccatgaaga ccggggtctg gggccgctcg ggcttccacg cccggagccc gagcacgagg
       61 ctgcggtggc gacgggcgat cgtgtcgagc tcgggcgccg ccgcggccag gctcatctcg
      121 tccgcgtcgc ccgcgcgcgt ccccgcgagg agcgggtcga cgagccgctc caggacctcg
      181 tcgccgaggc ggtgacgcac gagcgcgccg accgacacgt ccttgccggt gagcggcttc
      241 gagttgaaga ggtcgccgac gccgcgcagc gatccgcgga acgacagcag gccgccgcgg
      301 gccatcttca ccggcagggt cgggatgccg aggacgagcc cgcgcggcaa cggatggagc
      361 cgcccgcgcg accacacgag gcccccgaac accgcaggct tgatcaggtc gttgccgagc
      421 ccgatcgcac ggcagagctc gaccggccag gggtcgcgca cgaggaacga gtccggcccg
      481 ccttcgaccg cgacgccttc cagcgtcttg gtgacgatct tcccgccggc gtcgtccgcg
      541 gcctcgagca cgacgacgtc ggctccggcg cggtccagct cgcgggccgc gaccaagccc
      601 gtgatgccgc cgccgacgac ggcgacgacc ttgccgctca tccgggtgcg tcgcctgtct
      661 tgtcgagcag ccggctcacc acggttgcga gcacgtcgag gaacgcgggg tcgtcgttgg
      721 gcgactcggt ccgcaccagc tcgacgccga ggcgccgggc ggcgtcgacg cactcgacgt
      781 cgacgtcgta gaggacttcc aggtggtcgg agacgaaccc gcacggacag acgatcaccc
      841 cgcgggcacc ttcgccggcg agccgttcga tcacctcgag cacgtcgggg ccgagccacg
      901 ggtccgccgt ccggcccgcg ctctgccacc cgacggtgta cgactccaca cctgccgccg
      961 cggcgaccgc gcccgcggtc tcttgcagct gctcgggata cgggtcgccc ccgcgcagga
     1021 ttgcctccgg aaggctgtgc gcggtgaaca cgacgacgtg gcgatcgcga gcgtcgtccg
     1081 gtagccgcgc cgcggcctcg cgcacccgct gcgcgaggaa gtcgacgtag cccggttcga
     1141 cgtgccagct ctcgacgacg tcgaccggcg cgcccaaccc cgcctcgtcc cgcgcgagca
     1201 tgacccgccg ccggtagtcg ccgacgctca tcttcgagta gtgcggcgcg agcacgaggc
     1261 cgacgatccg gtccacgccg tcgtccgcca tcgcgcgcac cgcgtccggg atgaacggcg
     1321 gcgagtgctt ctgccccagg tacgtcggaa cgccgtcgag tctcttctcg agcccgatcc
     1381 tctgggcctc ggtgatctcc aacagcggcg accggccccc gatggcttcg tagcgagcgg
     1441 tcagctcggc gagcagctcc ggcgacggcg gacggccgtg gcggatgtcg gtgtagtagc
     1501 gctcgacgtc gtcgagcccg gccggcgtcc cgtacgccat cacgatcacg ccggacctca
     1561 ccattcgtgc accgcttcga ccagccgcgc cagcgtgtcc gggtccgtcg acggcaggac
     1621 tccgtgcccg aggttgaaga cgtgccgccg gcccccggtc tcgtcgagga tctcgtgcgc
     1681 gcgctcgcgc acgacctcga actgagcgag acacagcgcg gggtcgaggt tgccctgcag
     1741 cacgacgccg tctccgagcc gcgcgcgcgc ccagtcgagt gggac
//
LOCUS       DASQDO010000073         6803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_559039, whole genome shotgun sequence.
ACCESSION   DASQDO010000073 DASQDO010000000
VERSION     DASQDO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559039"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VEV43_02280"
     CDS             <1..532
                     /locus_tag="VEV43_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HYP22375.1"
                     /translation="DPERYRRLHVITGDVLEQWESVLETLERDPMALKDRLEWVAKRS
                     ELEAYSDRDGLSWDHPKLKLSDLQYHDVQREKGLYNKMAASGKIKRLVPEESIEFAID
                     HPPEDTRAYFRGECLRRFSNVIVAASWDALIFDVGDEPLRKVPTLEPTRGTKERVQAL
                     FDASPDAATLLKNLSA"
     gene            608..787
                     /locus_tag="VEV43_02285"
     CDS             608..787
                     /locus_tag="VEV43_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HYP22376.1"
                     /translation="MADQTRKPKPKQEDAPQATEETKAESKTDEVDELLDEIDEVLEQ
                     NAEEFVRSYVQKGGQ"
     gene            863..1255
                     /locus_tag="VEV43_02290"
     CDS             863..1255
                     /locus_tag="VEV43_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VII domain-containing protein"
                     /protein_id="HYP22377.1"
                     /translation="MVCTKCGLDRALDEFPRNKRGPYGRHWYCKACHSIQNRASRARN
                     GGARKYHIKRRYGLSLEDFDALLAMQGFLCPICLKRPAVHVDHDHSTGTVRGVLCEMC
                     NGGLGQFRDNPATIESAIAYLERPGGDV"
     gene            complement(1411..1659)
                     /locus_tag="VEV43_02295"
     CDS             complement(1411..1659)
                     /locus_tag="VEV43_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22378.1"
                     /translation="MRVASSGIMGPKIFDCPAEGRGIVLEAADALVAVEAEEAANLLR
                     SVRMIDVSGLGGLADSTDATLRSEEPVDPLTRDAVAML"
     gene            1634..2494
                     /gene="prcB"
                     /locus_tag="VEV43_02300"
     CDS             1634..2494
                     /gene="prcB"
                     /locus_tag="VEV43_02300"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HYP22379.1"
                     /translation="MIPDDATLTELLRMPVYGPGSSFSEVLKARTPDLLPPTEADGPF
                     HVPEGTTVLALRFGDGVIVAGDRRATEGLQIAHRSIEKVFAADDMSAVAIAGAAGPAV
                     EMVRLFQTELEHYEKVEGERLTLEGKANKLSQMIRANLPAAMQGLAVVPLFAGYDEGR
                     GQGRIFKYDITGGRYEEDDYHATGSGGKDARSSLKKRFRSDLSRDDAVKIAIEALFDA
                     ADEDVGTGGPDLMRGIYPTVASITGAGLVEIAEPEIERLFQELISERRDQMLMPETHT
                     VDVRRPAQES"
     gene            2498..3178
                     /gene="prcA"
                     /locus_tag="VEV43_02305"
     CDS             2498..3178
                     /gene="prcA"
                     /locus_tag="VEV43_02305"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HYP22380.1"
                     /translation="MATPYYVSPEQLMKDKADFARKGIARGKSVMAIEYSDGILFIAE
                     NPSATLHKISEIYDRIAFAGVGRFNEFEQLRIGGIRLVDVKGYSYSREDVTAKGLANA
                     YSQSLGNIFTSEVKPYECEVLVAAVGDSTETNELFHILYDGSVTDRQGWVAMGGQAEA
                     LGTYLKDKFPKKDPDLKAGLKLGHDALVSVEGREPTPSGLEVAVLDRTRARRKFRRLT
                     NEELKGLL"
     gene            3265..3420
                     /locus_tag="VEV43_02310"
     CDS             3265..3420
                     /locus_tag="VEV43_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22381.1"
                     /translation="MGENLVENVELPEPPLTGNPAIDRMVDWVRTNYNDLVTLCEANN
                     LPKPPHW"
     gene            complement(3428..6601)
                     /locus_tag="VEV43_02315"
     CDS             complement(3428..6601)
                     /locus_tag="VEV43_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYP22382.1"
                     /translation="MSACGRCGAATPAGARFCPGCGSPLSGVPAGDTLKTVSVVFTDL
                     VGSTALAERFDPESLHTLMREYFETIRRVVTRYGGSVEKFIGDAVVAVFGVPTLHEDD
                     ALRAVTAAAAIHRAIDELSARLDRDAGVTLEVRTGVNTGEVLSQPDGTSGRLVGDAVN
                     LAARLQQAAAPGQVLLGPSTQDLVAHRVRSEALEPFLVKGKAAPVRAHRLVEVLPAAR
                     GTAPKRTIVGRDREARLLRETLQRVVEEHTPHLFTVLGSAGIGKSRLVAELEDAGEAV
                     VLHGRCLPYGEGITYWPLAEIVQRAAGIDSGDPPDVARGKLVASAGGDPESETVAERM
                     AQVFALEESPGTTDDLSWAVRKYFEHLAVERPHVLVFDDVHWGETALLDLVEQLVDSL
                     ADVPLLIVCVARLELLDVRPAWGGGKLNASSLVLGPLKEPECRALIGSLLGESPSLDE
                     VTDAVFRVADGNPLFVEEMVSMLVDHGFLDRAGGPWAATRDLASLSVPPTIQALVSAR
                     LDRLTREEREVVGVAAVMGKVFASSAVGDLIGGRVDEALDRLTRKQLLAPEGTEFAGR
                     RTYSFRHLLIRDTAYEHLPRMKRASIHERYADWLVDALGDRVAEYDEIVGYHLEQAHQ
                     YQSNLGGLAAGGSNLATRAADRLAEGGRRALARQDVTAAVKLLSRAAELLPGDDEKRL
                     SVLPELGRALFESGSYAPAMEAFEEAIELGTKLGDERVATTSTVFNERVRVHSDPHVN
                     AEELESVAQHAIAVLSDLGDDYGLCYAWDLAAYAHDCAGRSDDALAAFRKAAEHAERS
                     GVRSLIGYQKRALVRSLAWGSGHVSEILALGGELLEWARKVGDRYSEARALLSLGQAH
                     AMLGEFDLARGYVAQQREVCADVSFAFIDASGAFERSQVELLAGDLDAAEAEARGGCE
                     TLQKMGEKGVLPTLQAHLADIAYARGQDGAALRLVETARGLSAPDDSLTAMRWRAVLA
                     KILARRGDVLAATELLDDARSIAAETGYLDWHAGLLVDVAEVMTTAQRPVDATKALRA
                     AIDLYGRKGNLVAQRAAEGRLRRVVPA"
     gene            6658..>6803
                     /locus_tag="VEV43_02320"
     CDS             6658..>6803
                     /locus_tag="VEV43_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HYP22383.1"
                     /translation="MEKRIFGTENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFL"
BASE COUNT         1168 a         2379 c         2255 g         1001 t
ORIGIN      
        1 cgacccggag cgctaccgcc gcctccacgt aatcaccggc gacgtgctgg agcagtggga
       61 gagcgtcctc gagacgctgg agcgcgaccc gatggcgctg aaggaccggc tcgagtgggt
      121 cgcgaagcgc tcggagctcg aggcctacag cgatcgcgat gggctgtcgt gggaccaccc
      181 caagctgaag ctcagcgacc tccagtacca cgacgtgcag cgtgagaagg gcctctacaa
      241 caagatggcc gcgtcgggga agatcaagcg gctcgtgccg gaggagtcga tcgagttcgc
      301 gatcgaccac ccgcccgagg acacccgggc gtacttccgg ggcgagtgcc tgcggcgctt
      361 ctcgaacgtg atcgtcgcgg cgtcgtggga cgcgctgatc ttcgacgtcg gcgacgagcc
      421 gctgcgcaag gtgccgacgc tcgagccgac ccgggggacc aaggagaggg tccaggccct
      481 gttcgacgcc tcgccggatg cggcgacctt gctcaagaac ctgagcgcgt agtccgacca
      541 gtcgtgtagg ggccttgtcg cacccgctgg gtacgattta ggcctgacct gtaaggagag
      601 gtggagcatg gccgaccaga cccgcaagcc gaagccgaag caagaagacg cgccgcaggc
      661 gacggaagag accaaggccg agtcgaagac cgacgaggtc gacgagctgc tggacgagat
      721 cgacgaggtg ctggagcaga acgccgagga gttcgtgcgt tcatacgtcc agaagggcgg
      781 ccaatgatgt ggagcgtagg cggctaagca cttagcgtaa gtacccgcgg caccgcgcgc
      841 cgcccgaacc gggaggtagc gcgtggtctg cacgaaatgc gggctcgata gggcactcga
      901 cgaattcccc cggaacaaga gggggccgta cggccggcac tggtactgca aggcctgcca
      961 cagcattcag aatcgcgcat cccgcgctcg gaacggcggc gcgcggaagt accacatcaa
     1021 gaggcgctac gggctttcac tcgaggactt cgacgccctc ctggcaatgc agggcttcct
     1081 atgccctatc tgtctaaagc gtccggccgt ccacgtcgac cacgatcact ccaccgggac
     1141 ggtgcgcggg gtcctctgtg agatgtgcaa cggaggcctc ggccaattcc gagacaaccc
     1201 agcaacgatc gaaagcgcga tcgcgtacct ggaacgtcca ggcggtgatg tctgatgcgt
     1261 tgtcctgagt gtcgcgagaa caaggacccg gaggacttcc cgcgccatcg agggagcaag
     1321 accggccgac acacgtactg caagccgtgc cacaacgaac gcaccaagcg atacaagaac
     1381 cggagtcacg gcaccgaaca gaactatctc ctagagcatc gctacggcat ctcgcgtgag
     1441 cgggtcgacc ggctcctcgc tgcgcagggt ggcgtctgtg ctatctgcaa gacccccgag
     1501 cccagacacg tcgatcattc gcacgctacg aagaaggttc gcggcctcct ctgcctcaac
     1561 tgcaaccagg gcatcggccg cttcgaggac gatcccgcga ccctccgccg ggcaatcgaa
     1621 tatcttcggc cccatgatcc ctgacgacgc caccctcacc gagctcctcc gcatgcccgt
     1681 ctacggcccc ggctcctcgt tctcggaggt gttgaaggcc cggaccccgg acctgctgcc
     1741 gcccaccgag gcggacgggc cgttccacgt gcccgagggg acgacggtgc tcgcgctccg
     1801 cttcggcgac ggcgtcatcg tcgccggcga ccgccgcgcc accgagggct tacagatcgc
     1861 gcaccgctcg atcgagaagg tcttcgccgc cgatgacatg tccgccgtcg ccatagcggg
     1921 cgcggccggg cccgcggtcg agatggtccg tctcttccag accgagctcg agcactacga
     1981 gaaggtcgag ggcgaacggc tcacgctcga gggcaaggcg aacaagctct cgcagatgat
     2041 ccgggcgaac cttcccgccg ccatgcaggg gctcgcagtc gtcccgctgt tcgccgggta
     2101 cgacgagggt cgggggcagg gacgcatctt caagtacgac atcaccggcg gccgctacga
     2161 agaggacgac tatcacgcaa ccggatccgg cggtaaggac gcgcgcagca gcctcaagaa
     2221 gcggttccgc tccgacctga gtcgcgacga cgccgtgaag atcgcgatcg aggcgctgtt
     2281 cgacgccgcc gacgaagacg tcggcacggg gggccccgac ctcatgcggg ggatctatcc
     2341 cacggttgcg tcgatcacgg gggccggtct cgtcgagatc gcggagcccg agatcgagcg
     2401 cctgttccag gagctcatct ccgaacgccg cgaccagatg ctgatgcccg agacccacac
     2461 cgtcgacgtc cgccgcccgg cgcaggagag ctgacccatg gctacccctt actacgtctc
     2521 gcccgagcag ctcatgaagg acaaggcgga cttcgcccgc aagggcatcg cgcgcggcaa
     2581 gtccgtcatg gcgatcgagt actccgacgg catcctgttc atcgccgaga acccgagcgc
     2641 gaccctccac aagatctccg agatctacga ccggatcgcg ttcgccggcg taggccgctt
     2701 caacgagttc gagcagctcc gcatcggcgg catccgcctg gtcgacgtga agggctactc
     2761 gtactcacgt gaggacgtca ctgccaaggg gctcgcgaac gcgtactcgc agtcgctcgg
     2821 caacatcttc acgtccgagg tcaagccgta cgaatgcgaa gtcctggtgg ccgcggtcgg
     2881 cgacagcacc gagaccaacg agctgttcca catcctctac gacggctccg tgaccgaccg
     2941 gcagggttgg gtcgcgatgg ggggccaggc cgaggccttg ggcacgtacc tcaaggacaa
     3001 gttcccgaag aaggaccccg acctgaaggc cggcctcaag ctgggccacg acgcactggt
     3061 ctcggtagag ggccgcgagc cgacgccgtc gggtctcgag gttgcggtcc tggaccggac
     3121 gagggcgagg cgcaagttcc gccggctgac gaacgaagag ctgaagggcc tgctgtaggt
     3181 gtcagccagc taactgcaag cagagcgagc ccgtccctat actcgatccc ggcatgcgac
     3241 tccagccttc ataaggagga cctgatgggc gagaacctcg tcgagaacgt ggagctgccc
     3301 gagcccccgc tgaccggcaa ccccgcgatc gaccggatgg tggactgggt gaggaccaac
     3361 tacaacgacc tcgtgaccct ttgcgaggcc aacaacctcc cgaagccgcc ccactggtaa
     3421 agaccgtcta ggcgggaacc acccgccgca gccttccctc ggcggcgcgc tgcgccacca
     3481 ggttgccctt ccgtccgtag agatcgattg ccgccctgag cgctttcgtg gcatcgacgg
     3541 gtctctgcgc cgtcgtcatc acctcggcga cgtcgacgag cagcccggcg tgccagtcga
     3601 ggtagcccgt ttccgcagct atcgatcgtg cgtcgtcgag aagctcggtc gcggcgagga
     3661 cgtcgccgcg cctggcgagg atctttgcca gcacggcgcg ccagcgcatc gccgtcagcg
     3721 agtcgtcggg ggcggacagt ccccgggcgg tttccacgag acgcagagcc gcgccgtcct
     3781 gaccacgcgc gtatgcgatg tcggccaggt gggcctgcag ggtgggcagg acgcccttct
     3841 cgcccatctt ctggagggtc tcacaaccgc ctcgcgcctc ggcctcggcc gcgtcgagat
     3901 cccccgccag caattccacc tgagaacgtt cgaacgcgcc cgaggcgtcg atgaacgcga
     3961 acgaaacgtc ggcacagacc tctcgctgct gcgcgacgta gccccgcgcg aggtcgaact
     4021 cgccgagcat ggcgtgagcc tgaccgaggc tgagaagggc gcgcgcctcc gaataccggt
     4081 cgccgacctt ccgcgcccac tcgaggagct cgcctccgag agcgaggatc tccgagacgt
     4141 gcccggagcc ccacgcaagg gaacgcacca gcgcccgctt ctggtagccg atcagcgacc
     4201 ggaccccgga cctctctgcg tgctcggcag ctttccggaa ggccgcgagg gcgtcgtccg
     4261 accggccggc acagtcgtgc gcatatgccg cgaggtccca ggcgtagcag aggccgtagt
     4321 cgtcgccgag gtcgctcagg acggcgatcg cgtgttgcgc gacgctctcg agctcttcgg
     4381 cgttcacgtg cgggtcgctg tgcacgcgga cgcgctcgtt gaagaccgtc gacgtggtcg
     4441 cgacgcgctc gtcgccgagc ttcgtcccga gctcgatggc ttcttcgaat gcctccatcg
     4501 ccggtgcata actgccgctt tcgaagagcg cccgtccgag ctcggggagc acgctcagcc
     4561 tcttctcgtc gtcacccggg agcagctcgg ctgctctcga cagcagcttc acggcagcgg
     4621 tgacgtcttg gcgtgcgaga gctcggcgac cgccctcggc caaacggtcc gccgcgcgcg
     4681 tcgccaggtt cgaaccgccc gcggcaagcc ccccgaggtt gctctggtac tggtgggctt
     4741 gctccaggtg atagcccacg atctcgtcgt actccgcgac gcggtcgccc agggcgtcta
     4801 cgagccagtc ggcatagcgc tcgtggatgg acgccctctt catcctcggc aggtgctcgt
     4861 acgctgtgtc ccggatgagc aggtgccgga acgagtacgt acgccggcct gcgaactccg
     4921 tcccttccgg cgcgagcagc tgctttcgag tgaggcggtc gagggcttcg tcgacgcgtc
     4981 cccctatcag atcgccgact gcggacgacg cgaacacctt gcccatcacg gccgcgaccc
     5041 cgaccacctc acgctcctcg cgcgtcagcc ggtcgaggcg cgccgatacg agcgcctgga
     5101 tcgtcggggg aacgcttaga gacgcgaggt cgcgggtggc ggcccatggc cctcccgcgc
     5161 ggtcgagaaa gccatggtcg accagcatcg agaccatctc ttccacgaag agcgggttcc
     5221 cgtcggctac gcggaacacc gcgtccgtca cctcgtccag cgacggcgac tcgcccagca
     5281 acgacccgat cagggcccgg cactccggct ccttcagggg gcccagcacc aggctcgacg
     5341 cgttcagctt cccgccgccc catgccggcc tcacgtcgag cagctccagc cgcgccacgc
     5401 agacgatcag cagcggtacg tcggccagcg agtcgaccag ctgctcgacg agatcgagca
     5461 gcgcggtctc gccccagtgc acgtcgtcga agaccagcac gtgcggcctc tcgaccgcca
     5521 ggtgctcgaa gtacttccgg accgcccagg agaggtcgtc cgtcgtcccg gggctctcct
     5581 ccagcgcgaa cacctgcgcc atccgctccg cgaccgtctc gctctccggg tcaccgcccg
     5641 ccgaggcgac gagcttcccg cgcgcgacgt cgggagggtc ccccgagtcg atccccgcgg
     5701 ccctctggac gatctcggcg agcggccagt acgtgatgcc ctctccgtag gggaggcacc
     5761 ggccgtgcag cacgacggcc tcgccggcgt cctcgagctc ggcgacgagg cgcgacttcc
     5821 cgatgccggc cgagccgagg accgtgaaca ggtgcggcgt gtgttcctcg accactcgtt
     5881 gcagcgtctc gcgcaacagc cgcgcctccc ggtcgcggcc gacgatcgtc cgcttgggcg
     5941 cggttccccg tgcggcggga aggacctcga cgagacggtg cgctcgcacc ggcgcggcct
     6001 tgcccttcac gaggaacggc tccaacgcct cgctgcggac ccgatgcgcg acgaggtcct
     6061 gcgtggacgg cccgaggagg acttgtccgg gcgcggccgc ctgctgcagg cgcgcggcga
     6121 ggttcaccgc gtcgccgacg agccgtccgg aggtgccgtc gggctgggac aggacctccc
     6181 cggtgttcac gccggtcctc acctcgagcg tgacgccggc gtcgcggtcc aggcgcgcgc
     6241 tcagctcgtc gatcgcgcga tggatcgcgg ccgccgcggt gaccgcgcgc agcgcgtcgt
     6301 cctcgtgcag cgtcgggact ccgaagacgg ctacgaccgc gtctccgatg aacttctcga
     6361 cgctcccccc gtacctcgtc acgacccgcc ggatcgtctc gaagtactcc cgcatcagag
     6421 tgtggagcga ttcggggtcg aacctctccg ccagcgcggt cgagccgacg aggtcggtga
     6481 agacgacgga gacggtcttg agggtgtcgc cggccgggac gccggaaagc ggcgacccgc
     6541 agcccgggca gaacctggcc ccggcggggg tggcggcgcc gcaccgacca cacgcgctca
     6601 cggggcgaag actaccgaaa gcccctggac cgggcggttt acgtacacaa tgggccggtg
     6661 gagaagcgca tcttcgggac cgagaacgaa tacggcgtca cctgcacgtt ccggggccag
     6721 cgccggctgt cgcccgacga ggtcgcgcgc tacctcttcc gcagggtcgt gtcgtggggc
     6781 cgctcgtcga acgtgttcct cga
//
LOCUS       DASQDO010000074         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_69408, whole genome shotgun sequence.
ACCESSION   DASQDO010000074 DASQDO010000000
VERSION     DASQDO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69408"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..267
                     /locus_tag="VEV43_02325"
     CDS             82..267
                     /locus_tag="VEV43_02325"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYP22384.1"
                     /translation="MKKLELNKETLRDLTDVDLAEVAGGAYTTGCQGPTTVLTTTQTP
                     ACPSGATWFAPCDYEDQ"
     gene            293..475
                     /locus_tag="VEV43_02330"
     CDS             293..475
                     /locus_tag="VEV43_02330"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYP22385.1"
                     /translation="MAKKLELNKETLRDLSEVDLAEVAGGASTGTCPKTTGTTGLTTQ
                     TPVCPSGATWFASCGE"
     gene            507..692
                     /locus_tag="VEV43_02335"
     CDS             507..692
                     /locus_tag="VEV43_02335"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYP22386.1"
                     /translation="MSKKLQLNKETLRDLSETSLQEVSGGKGAVTGLCGGDTGTFVCP
                     SGATWFWDCQSVQVSCG"
     gene            758..2017
                     /locus_tag="VEV43_02340"
     CDS             758..2017
                     /locus_tag="VEV43_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HYP22387.1"
                     /translation="MTWEPVLEGELRERALEAALDLGSALAESFAADEAPASDGDPSL
                     SRGWTGTAVAMRHLDAAFPGEGFAAVSDAAIDRAVDAVAEQRMSPGLLQGFPGIAWAV
                     EHLSGNDGAEDANEAVDDALREMLGAARWTGLYDFVSGLVGVGVYALRRLPAASGRRL
                     LELVVERLEQWSEPGEPGTTWFTRPELIPPTARERVPEGYYNLGLAHGVPGVIALLAQ
                     ARRAGVATATAAPLLEGAVEWVLAQALPQGGRATWPYFVGPGIDPRPARLAWCYGDPG
                     VIVSLLAAADALGRPDWEEAAGAALSRAVARPPGSSDVQGASLCHGAMGLAHIWNRMW
                     QRSRSDELRESIAYWTTVALDLRTHPDGLAGYSVWDPGSDEEEGGDVQGPGLLAGIAG
                     IALALVAAATDLEPAWDSHLLLSEPAR"
     gene            2014..>3143
                     /locus_tag="VEV43_02345"
     CDS             2014..>3143
                     /locus_tag="VEV43_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HYP22388.1"
                     /translation="MRHEAVPNGSFVLRTPLYSIAEALAWSEGLAAAGSPDDPEALAR
                     DRGVLRDRLRAFYGRAEVQEALHLASPKLARRSRAFLDGADDPKVERPLIRYFLRMCT
                     RPTPFGTFAGSAVGRLGDATRLVVAPVSQNARRTRLDHLYLTALALGLEDRFRRKLTY
                     RVNSSVYTTAGKLRYAEASMRGGRSYSLVAVDPNEAVVAVLDRARDGATFDELAGVLV
                     DEDVTYDEAAEFVEELIASQLLESTLTPPITGPEAIAALIEELGAVEGAEEERGVLAD
                     VRAELEKLDAEGVGAPVDRYERIFSMVERLPAEAAENSFFQTDMVTALDEATIGPKVV
                     DEILRATAAVHRIVGKSSTGALERWRNEFVARYETREVPLVEA"
BASE COUNT          435 a         1115 c         1171 g          422 t
ORIGIN      
        1 gggagggaaa cggggtttgc gtgtataagt tctcgggcac tcgcaaagcg agcatctatc
       61 gcgcgcctga aaggggatgg gatgaagaag ctggagctca acaaggagac cctccgggac
      121 ctgaccgacg tcgacctggc cgaggtggcc ggcggcgcgt acacgacggg gtgccagggc
      181 ccgaccaccg tcctcaccac gacgcagacc cccgcgtgcc cgtcgggcgc gacgtggttc
      241 gcgccctgcg actacgaaga ccagtaagcc cgactgcgaa gggagccaca cgatggccaa
      301 gaagctcgag ctcaacaagg agacgctgcg ggacctctcc gaggtcgacc tcgccgaggt
      361 cgccggcggt gcctccaccg gcacgtgccc gaagacgacc gggacgaccg gcctgacgac
      421 gcagacgccc gtgtgcccgt cgggcgctac ctggttcgcc agctgcggcg agtagctcgg
      481 acacgcggac cgaaaggagc ggacgcatga gcaagaagct ccagctcaac aaggagaccc
      541 tgcgggacct ctccgagacg agcctccagg aggtctcggg cggcaagggc gcggtcaccg
      601 gcctgtgcgg cggcgacacc ggcacgttcg tatgcccgtc gggcgcgacc tggttctggg
      661 actgccagag cgttcaggtg tcctgcggct gagcccgcac gttcgatccg gatcacccac
      721 gagcggcctt cgggccgctc gtgttgatat ggggcccgtg acgtgggagc cggtcctcga
      781 gggtgagctg cgcgagcgtg cgctcgaggc cgcgctcgat ctcggcagcg ccctcgccga
      841 gtcgttcgcg gccgacgagg ccccggcgtc cgacggcgac ccgtcgctgt cgcgcggctg
      901 gaccggcacc gcggtcgcga tgcgccacct cgacgcggcc ttccccggtg agggcttcgc
      961 agcggtatcg gacgccgcca tcgaccgcgc cgtcgacgcg gtcgccgagc agaggatgag
     1021 ccccgggctc ttgcagggct tccccgggat cgcgtgggcg gtcgagcacc tcagcgggaa
     1081 cgacggcgcc gaggacgcga acgaggccgt cgacgacgcg ttgcgcgaga tgctcggcgc
     1141 cgctcgctgg accggtctct acgacttcgt gagcgggctc gtcggcgtgg gcgtctacgc
     1201 gctgcggcgg ctgccggcgg cttcgggacg caggctgctc gagctcgtcg tcgagcggct
     1261 cgagcagtgg tcggagcccg gggagcccgg caccacctgg ttcacgcgcc ccgagctgat
     1321 cccgcccacg gcgcgcgagc gcgtgcccga gggctactac aacctcggcc tcgcgcacgg
     1381 cgtccccggc gtcatagcgc tgctcgcgca ggcccggcgc gcgggcgtgg cgacggcgac
     1441 cgcggccccc cttctcgaag gcgcggtcga gtgggtcctg gcgcaggccc tcccgcaggg
     1501 gggccgcgcg acgtggccgt acttcgtcgg ccccggcatc gacccccggc ccgcgcggct
     1561 cgcgtggtgc tacggcgacc ccggcgtgat cgtgtcgctg ctggccgcgg ccgacgcgct
     1621 cggacggccg gactgggagg aggctgcagg cgcggcgctg tcgcgcgcgg tcgcccggcc
     1681 cccgggctcg agcgacgtcc agggcgcgag cctctgtcac ggcgccatgg ggctcgctca
     1741 catctggaac cggatgtggc agcgatcgcg ctccgacgag ctgcgcgagt cgatcgcgta
     1801 ctggacgacg gtcgcgctcg acctccgcac gcatcccgac gggctcgccg gctattcggt
     1861 gtgggacccc ggctccgacg aggaggaagg tggcgacgtg cagggtccgg gtctgctcgc
     1921 cggcatcgcc gggatcgcgc tcgcgctggt cgccgcggcg acggacctcg agccggcgtg
     1981 ggactcgcac ctgctgctgt cggagccggc gcggtgaggc acgaagcggt ccctaacggc
     2041 tcgttcgtcc tgcggacgcc gctgtactcg atcgccgaag ccctcgcgtg gagcgagggt
     2101 ctcgcggcgg cggggtcgcc ggacgatccc gaggccctgg cgcgcgaccg cggcgtgctg
     2161 cgcgaccgcc tgcgcgcgtt ctacgggcgg gccgaggtgc aggaggccct acacctcgcg
     2221 tcgccgaagc tcgcgcgccg gtcgcgcgcg ttcctcgacg gcgcggacga cccgaaggtc
     2281 gagcgcccgc tcatccgtta cttcctgcgg atgtgcacgc gcccgacgcc gttcgggacg
     2341 ttcgcaggaa gtgccgtggg ccggctcggc gacgcgaccc ggctcgtcgt cgcgcccgtg
     2401 tcgcagaacg cccgccggac gcgcctcgac cacctgtacc tgaccgctct cgcgctcggc
     2461 ctcgaggacc gcttccgccg caagctcacg taccgcgtca actccagcgt ctacacgacc
     2521 gccgggaagc tccgctacgc cgaggcgagc atgcgcggcg ggcgcagcta cagcctcgtc
     2581 gcggtggacc cgaacgaggc cgtcgtcgcg gtgctcgacc gggcgcgcga cggcgcgacg
     2641 ttcgacgagc tcgccggggt cctcgtggac gaggacgtca cctacgacga agcggcggag
     2701 ttcgtggagg agctgatcgc gtcgcagctg ctcgagtcga cgctcactcc tccgatcacg
     2761 gggccggagg cgatcgcggc cctgatcgag gagctcgggg ccgtcgaggg ggccgaggaa
     2821 gagcgcgggg tgctcgccga cgtgcgggcc gagctcgaga agctcgacgc cgagggcgtc
     2881 ggtgcacccg tcgaccgcta cgaacggatc ttctcgatgg tcgagcgcct tcccgcggag
     2941 gcggccgaga actcgttctt ccagaccgac atggtgaccg ccctggacga ggcgacgatc
     3001 ggcccgaagg tcgtcgacga gatcctgcgg gccaccgctg cggtccatcg gatcgtcggc
     3061 aagtcctcga cgggtgcgct cgagcgctgg cgcaacgagt tcgtcgcccg ctacgagacg
     3121 cgggaggtcc cgctcgtcga ggc
//
LOCUS       DASQDO010000075         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1170981, whole genome shotgun sequence.
ACCESSION   DASQDO010000075 DASQDO010000000
VERSION     DASQDO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170981"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VEV43_02350"
     CDS             <1..319
                     /locus_tag="VEV43_02350"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HYP22389.1"
                     /translation="GPRPGAAVEKLTALSRGAPEWAELDAGSWGVVTRTSAGRLETLL
                     ALYARSAIALVAEEAERLRRCPAPSCGMFYVSTRGSQRWCSTQCGTRARVARHYRSRR
                     QGS"
     gene            319..957
                     /locus_tag="VEV43_02355"
     CDS             319..957
                     /locus_tag="VEV43_02355"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HYP22390.1"
                     /translation="MEVPEGGVVTGPDGVTRCWWCAGNPLYERYHDTEWGRPVDDDRR
                     LFEKLSLEGFMSGLSWLTILRKREDFRRAFKSFDLEAVARFNSRSVERLLGDASIVRH
                     RGKIESTINNARRCIELVEEQGSLARYVWSFEPEARSRPKTLEWATLAQRTTSPEAVA
                     LSKDLKKRGWTYVGPTTVHSFMEAMGLVNDHVTPCDMRAEAEKERRAFERPV"
     gene            complement(1043..1246)
                     /locus_tag="VEV43_02360"
     CDS             complement(1043..1246)
                     /locus_tag="VEV43_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22391.1"
                     /translation="MLANVVFGILGGLVGLALSATPALFGGDLSVALLVISFGMGVFA
                     AYVAIQRHGSAAEVWRKLRSAPR"
     gene            complement(1256..>1615)
                     /locus_tag="VEV43_02365"
     CDS             complement(1256..>1615)
                     /locus_tag="VEV43_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYP22392.1"
                     /translation="GVGSLLLGLYSADGVLHNVGHTSSFKAAERRALLEELKPLEGGT
                     SFVGGRAPGGPSRWTGANEKTWVPLEPSLVCEVSFDHMQGERFRHAATFVRWRPEKEP
                     RDCDFVQLGALFKGNRG"
BASE COUNT          256 a          564 c          576 g          219 t
ORIGIN      
        1 cggcccccgg ccgggggccg cggtcgagaa gctgaccgcg ctgagccgtg gtgctccgga
       61 gtgggccgag ctcgacgcgg ggtcgtgggg agtggtgacg cggacgagcg cggggaggct
      121 cgagacgttg ctggccctgt acgcgcgctc ggcgatcgct ctcgtcgccg aggaggcaga
      181 gcggttgcgc cgctgcccgg ccccctcgtg cggcatgttc tacgtaagca cgcgcggctc
      241 ccagcggtgg tgctccacgc agtgcgggac gcgtgcccgg gtcgcccggc actaccgaag
      301 ccggcggcag gggagctagt ggaggtcccc gagggcggcg tcgtcaccgg cccggacggc
      361 gtcacgcgtt gctggtggtg cgccggcaac cccctgtacg agcgctacca cgacaccgaa
      421 tgggggcgcc cggtcgacga cgacaggcgt ctgttcgaga agctctcgct cgagggcttc
      481 atgtcggggc tgagctggct cacgatcctg cgcaagcgcg aggacttccg ccgcgcgttc
      541 aagtcgttcg acctcgaagc ggtcgctcgc ttcaactcgc ggagcgtcga gcgcctgctg
      601 ggcgacgcgt cgatcgtgcg ccaccgaggc aagatcgagt cgacgatcaa caacgcgcgg
      661 cgctgtatcg agctggtcga ggagcagggg agcctcgccc gctacgtgtg gtcgttcgag
      721 ccggaggcgc gttcacggcc gaagaccttg gaatgggcga cgctcgcgca gcggacgacg
      781 agtccggagg cggtcgcgct gagcaaggac ctgaagaagc gcgggtggac ctacgtgggc
      841 ccgacgaccg tgcactcgtt catggaggcg atgggcctcg tcaacgacca cgtgacgccg
      901 tgcgacatgc gggccgaagc cgagaaggag cgccgcgcct tcgagcgtcc cgtctagtgg
      961 ccaggcctct tttccacgcc tcggaatttg tcagacattt gtctgacaaa accagagggg
     1021 cgcgcgcggc ccccgacacg cgttagcggg gcgccgagcg gagcttgcgc cagacctcgg
     1081 ctgcagaccc gtgccgctga atggcgacgt aggcggcgaa gacgcccatc ccgaacgaga
     1141 tcaccagcag ggcgacggac aggtcgccgc cgaacaacgc gggcgtcgcc gacagcgcga
     1201 gcccgacgag ccccccgagg atcccgaaca cgacgtttgc gagcacggcc cgagcctagc
     1261 cccggttccc cttgaacagg gcccccagct gcacgaagtc gcagtcccga ggctccttct
     1321 cggggcgcca ccgcacgaac gtcgccgcgt ggcggaaacg ctcgccctgc atgtggtcga
     1381 acgagacctc gcacacgagg ctcggctcca gcgggaccca cgtcttctcg ttcgctcccg
     1441 tccagcggct cggccccccc ggcgcccgcc cgccgacgaa gctcgtcccg ccctcgagcg
     1501 gcttcagctc ctccagcagc gcccgtcgct cggccgcctt gaaggaagac gtgtgcccga
     1561 cgttgtggag gacgccgtcc gccgagtaga ggcccagcag cagagaaccg acccc
//
LOCUS       DASQDO010000076         6097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_131345, whole genome shotgun sequence.
ACCESSION   DASQDO010000076 DASQDO010000000
VERSION     DASQDO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131345"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="VEV43_02370"
     CDS             complement(<1..1908)
                     /locus_tag="VEV43_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22393.1"
                     /translation="MSIGITVSVLVLLAVASVPKMMSARDDRLLQRQPIAASGTPSFL
                     YAKTTDWWEGRTIARVNVARLRGEARPPGVSELPNTGEAFVSPALKQLIESDETGVLG
                     RRYGRSAGTIGEPGLASPDELIGYVGVDASTLRRNDGAFVAGDAFGPSEGGLAAISDE
                     RINPTSTTAAIGVLLVAMLVVFLSVCVRLSSVSQERRLSAMRLAGASANQARIVLAGE
                     LMLATVGGALLGLVAYLALAAMSEGWHVGSATWFAEDVRPGFASAVVLIGVPFLGHVV
                     AQAGARRSIRDAFHVRRDTRQGVASTWRLVPGLVGLTALAVVVSDSLRPSGWGANEIQ
                     TVNLSAIVLCVVGFPLALPVVFRLLATALARSGYVPLHLAARRLGHEPSSATRVAVGA
                     GVAVLIVGVVGSYVADIAAADTEFLSRRDLAARAGLVRVDAALSERHVNELGNVTGVD
                     SAVGLELMRIVRQPPSPVEELAWVVDCDDAARLLGGKDANPDCPDGVVRVSLGEGVDT
                     PEVFCRSDDTCVEVPPLQTGESVRLRPLGGGPMPERVDATVPERTTTIASAPLFLPEL
                     TTVALLVPPSETGRRGVEDPFETVLLATDGSDETEQAIRDHVVAADAVAEVTTQRAVF
                     ELRTQRRDFERA"
     gene            complement(1980..2684)
                     /locus_tag="VEV43_02375"
     CDS             complement(1980..2684)
                     /locus_tag="VEV43_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP22394.1"
                     /translation="MDDEQLLVAKDLSLTYGATQALVSASITVRPAEAVGVIGPSGSG
                     KSSLLHCLSGILRPSSGSVRFLGKALEDSSDEELSLLRRTEFGFVFQFGDLVPELSLV
                     ENVALPLRFSSVKKKKAEGQALEVLDELAIGDLAARRPSQVSGGEMQRAAIARSLVHE
                     PRIVFADEPTGALDSATGEQVLEVILDRTRARGASLVLVTHDRTVAARADRIVEVRDG
                     RTVGLGAVKSAGSSSR"
     gene            complement(2763..3188)
                     /locus_tag="VEV43_02380"
     CDS             complement(2763..3188)
                     /locus_tag="VEV43_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22395.1"
                     /translation="MKKRLMALGAASAMLLVFMAGPAHADLADNTWGPFARSTTGASV
                     SNCWLYSWKGNVHDQYGRVECNLKDTMADGDAVYVEWVQDGYAAISLYNHGGNGTTKF
                     VADTRYNGDGSFGTLKFRVCRDQGFPSSDNCSDWVIRHP"
     gene            complement(3378..3659)
                     /locus_tag="VEV43_02385"
     CDS             complement(3378..3659)
                     /locus_tag="VEV43_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HYP22396.1"
                     /translation="MAKKTPQPDGSVPKKPSLAQTLGAMVAGILAVKLATFIVTTVWR
                     LATKEDPPQVDQDVPIKKKAAWLALMGAATGAARQAARDVIKPPTSGPA"
     gene            complement(3732..4928)
                     /locus_tag="VEV43_02390"
     CDS             complement(3732..4928)
                     /locus_tag="VEV43_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HYP22397.1"
                     /translation="MDSEGPRTVKGLLAEIKDSSELMVDLAYAAVFFADADLAKEVRR
                     LEDRMFDHLHQLRKLAILAARSPEDAEHMAGVLEIASAVEKIADAAEDISHVVSSGLG
                     INDDLLRDLRHSDEIVSRIKVREGSRSVGQSLRELNLPVEIGMWVIACRRGGDWDFDP
                     GPEYVIASGDVLLVRGPEDGVILAREIAGAPSLPEPPEPPEIVMSDLDRAVDLLVEMK
                     NSAEVAVGLAYSALLFNDQALAAEVSNLEARSDAIHDELESWVLRSALEARNPDELRG
                     LLRLASASETIFDSAREMTRLVEAGEELHPVIAMALVESDEFGHETIVGAGSPADGRT
                     IKDLCVETETGMFVLAVQRGRRWTYRPKPRFQFEAGDRIIAIGPAEGGEELDELVGME
                     RELAEA"
     gene            complement(4954..>6097)
                     /locus_tag="VEV43_02395"
     CDS             complement(4954..>6097)
                     /locus_tag="VEV43_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYP22398.1"
                     /translation="IASLTSLVAGLTLAGMSHRIDEVDGLFVLIPVSIGMRGNIFGAL
                     AARLGTSIHTGLFEVSRAREGALVQNVYAATLLTVATSVTMGLLARAIAGLLGIETVS
                     ALDFIVVALVGGLLSSVVVLAVTIYVSITSYRRGWDLDSVGAPLITAIGDVVTLPCLL
                     LASYLVHVRVVTVTIGIAALVAGGASLFLGWTSSRSVARRIVRESFPVLCIAIVLDVL
                     AGAVVEPRVDEVFLKFSAFLIIVPGFLENTGALGAILAARLGSKLHLGVVTPTRRPDP
                     AALLDGMIVLALGITVYVVTAVATLGLAEVTGAGYPGVFKFVAVVISGGVLATCVAAV
                     IGYYAATTTFRFGFDPDNHTIPLVTSGMDLMGVICLVIALVVFGVA"
BASE COUNT         1038 a         2160 c         1994 g          905 t
ORIGIN      
        1 ggctctctcg aagtcacggc gctgggtcct gagctcgaag accgcccgtt gcgtcgtgac
       61 ctcggcgacg gcgtccgcgg cgactacgtg gtcacggatc gcttgttcgg tctcgtccga
      121 accgtccgtt gcgagcagga cggtctcgaa cgggtcctcc acgccgcgtc gtccggtttc
      181 ggaaggtggg accagcagcg cgaccgtggt cagctccgga aggaacaacg gcgcgctcgc
      241 gatcgtcgtg gttcgttcgg gcaccgtcgc gtcgacgcgc tctggcatcg gtccgcctcc
      301 tagcggacgg agacggacgg actctcccgt ctgaagtggt gggacctcga cgcaggtgtc
      361 gtccgatctg cagaagacct ccggagtgtc gacgccctcg cccaacgaaa ccctcacgac
      421 gccgtcgggg caatcggggt tggcgtcttt accgccgaga aggcgcgccg cgtcgtcgca
      481 atcgacgacc caggcgagct cctcgaccgg cgacggcggc tgtctgacga tccgcatcag
      541 ctcgaggccg accgcggagt ccacacccgt gacgttgccg agctcgttga cgtggcgctc
      601 ggagagggcc gcgtcgacgc ggacgagacc tgcccgcgcc gcgaggtcgc gccggctcaa
      661 gaactcggta tcggccgcgg ctatgtctgc gacgtagctg cccacgacgc cgacgatcag
      721 aaccgcaacc cctgctccca ccgcgaccct cgtggcactg ctcggctcgt gtccgagccg
      781 tctggccgcg agatgcagcg gcacgtaccc gctgcgcgcc agcgccgtcg ccaggagccg
      841 gaagacgacc gggagcgcga gagggaatcc gacgacacac aagacgatcg cgctgaggtt
      901 cacggtctgg atctcgttcg cgccccaacc cgacggacgc aacgagtcgc tgaccacgac
      961 ggccagcgcg gtcaggccca ccagaccggg gaccagcctc cacgtcgaag cgacaccctg
     1021 acgcgtgtcc ctgcgaacgt ggaacgcgtc ccggatggac cttcgtgcgc ccgcctgagc
     1081 cacgacgtgt ccgaggaagg gaactcctat aaggacgacc gcgctcgcga acccgggccg
     1141 gacgtcctcg gcgaaccacg tcgcgctgcc tacgtgccag ccttcggaca tcgccgcgag
     1201 cgcgagatat gcgacgagcc ccagcagtgc gcccccgacc gtcgcgagca tcaactcgcc
     1261 cgccaggacg atgcgggcct ggttcgccga cgcgccggcg agcctcatcg ccgacagacg
     1321 ccgctcttgc gataccgacg acagccgaac gcacaccgag aggaagacga cgagcatcgc
     1381 gacgagcagc acgccgatcg ccgcggtcgt cgacgtcggg ttgatgcgct cgtcggagat
     1441 cgccgcgagg cccccttcgc tcggaccgaa ggcgtctccc gccacgaacg ctccgtcgtt
     1501 tcggcgcaga gtggacgcgt cgacgccgac gtatccgatt agctcgtcgg gagacgccag
     1561 ccccggctcc ccgatcgtcc ccgcgctccg accgtagcga cggcccaaca cccccgtctc
     1621 gtccgactcg atcagctgct tcaacgcggg cgatacgaac gcctcaccgg tgttcgggag
     1681 ctcggacacc ccgggaggac gagcctcccc tcggagacga gcgacgttca cgcgcgcgat
     1741 cgttcgccct tcccaccagt ccgtcgtctt ggcgtagagg aaggagggcg tcccggacgc
     1801 tgcgatcggt tgacgctgca ggagccggtc gtcgcgggcc gacatcatct tcgggacgct
     1861 cgcgaccgcc aggaggacga gcacgctgac ggtgatcccg atcgacatca aaccggtacg
     1921 agtacggctc cggcgatctc cgagtagccc tagtcgcgcg ccgaacagca gatgccggat
     1981 cacctgctcg aggacccggc ggacttgacc gccccgaggc cgacggtccg cccgtcgcgg
     2041 acctcgacga ttcggtcggc gcgagctgcc accgtgcgat cgtgagtcac caggacgagg
     2101 ctcgcgccgc gagcccgcgt cctgtcgagg atcacttcga ggacctgctc gccggtagcc
     2161 gagtcgagcg cacccgtcgg ctcgtcggcg aacacgatcc ttggctcgtg gacgaggctg
     2221 cgcgcgatag cggctcgctg catctcaccg ccggagacct gcgacggacg ccgcgctgcg
     2281 aggtctccga tcgcgagctc gtccagcacc tcgagagcct gcccttcggc cttcttcttc
     2341 ttgaccgaag agaagcgcag cggcagagcg acgttctcga cgagggagag ctccgggacg
     2401 aggtcgccga actggaagac gaacccgaac tcggtgcgcc ggaggagact cagctcttcg
     2461 tcgctcgagt cctccagcgc cttcccgagg aaccgaaccg atcccgagct agggcgcagg
     2521 atccccgaaa ggcaatggag caacgacgac ttgcccgacc cgctcggccc gatcactcct
     2581 acggcctcgg ccggccgtac cgtgatcgat gcactgacca gcgcctgcgt cgcgccgtac
     2641 gtcaacgaga gatccttcgc cactaacagc tgttcgtcgt ccaccccgcc cccgtccgct
     2701 ctagaagaga aaaatgggcg tggcgctcat gagcgccacg cccattagat caaccctgtg
     2761 tcctagggat gccggatgac ccagtcggag cagttgtccg agctcgggaa cccctggtcg
     2821 cggcacaccc tgaacttcag cgtcccgaac gacccgtcgc cgttgtatcg agtgtctgcg
     2881 acgaacttcg tggtgccgtt cccgccgtgg ttgtagagcg agatcgccgc gtacccgtcc
     2941 tggacccact cgacgtagac cgcgtcgccg tcggccatcg tgtccttgag gttgcactcg
     3001 acacggccgt actggtcgtg cacgttgccc ttccagctgt agagccagca gttgctcacc
     3061 gaggctccgg tggtagaacg ggcgaacggg ccccacgtgt tgtcggcgag gtccgcgtgc
     3121 gcgggccctg ccatgaacac gagcagcatg gccgacgccg cccccaatgc catcaagcgc
     3181 ttcttcatct cgactccccc tctgggcact tggcccagct cctgcctgcg tggagtcata
     3241 cgacgcttta tttgcccgag ggtcaacgct tgtgcacaca tatggacaaa gctgactaca
     3301 aatctagtca cacttcgcgt taagtaaaaa acacgcgttc tggacgaaga cggccacgca
     3361 cgagcgcggc ggagatgtta cgcggggccg gacgtcggcg gcttgatcac gtcgcgcgca
     3421 gcctggcgcg cggcccccgt ggcggcgccc atcaatgcca gccacgctgc cttcttcttt
     3481 atcgggacgt cctggtcgac ctgcggcgga tcctctttcg tcgcgaggcg ccacaccgtc
     3541 gtcacgatga acgtcgcgag cttgaccgcg aggatgccgg cgaccatcgc gccgagcgtc
     3601 tgggcgagcg acggtttctt gggaaccgag ccgtccggct gcggggtctt cttggccacg
     3661 tcactccttt gcgaaaggcg actgcgaaag cgggtcggta cgtcttcaac ctatgcccgc
     3721 agaacggcgg cttaggcctc cgcgagctcc cgctccatcc cgacgagctc gtccagctcc
     3781 tcgccgccct cggccggccc gatcgcgatg atccggtccc cggcctcgaa ctggaagcgc
     3841 ggcttcgggc ggtaggtcca ccggcgcccc cgctggacgg cgaggacgaa catcccggtc
     3901 tcggtctcga cgcagaggtc cttgatcgtc ctgccgtcgg ccggcgatcc cgcgccgacg
     3961 atcgtctcgt ggccgaactc gtcggattcc acgagcgcca tggcgatcac cgggtgcagc
     4021 tcctcaccgg cttcgaccag acgcgtcatc tcgcgggccg agtcgaagat cgtctcggac
     4081 gcgctcgcaa gacgcaggag gccgcgcagc tcgtcggggt tgcgcgcctc gagcgcgctg
     4141 cggagcaccc acgactcgag ctcgtcgtgg atcgcgtccg aacgggcctc gaggttcgac
     4201 acctcggccg cgagggcctg gtcgttgaac agcaacgcgg agtacgcgag cccgaccgcc
     4261 acctcggcgg agttcttcat ctcgacgagc aggtccaccg cgcggtcgag gtccgacatc
     4321 acgatctcgg gcggctcggg aggctcgggc aaggacggcg cgcccgcgat ctcgcgcgcg
     4381 aggatcacgc cgtcttccgg cccccgcacg agcagcacgt ccccgctcgc gatcacgtac
     4441 tcgggccccg ggtcgaagtc ccagtcgcca ccgcgccggc atgcgatcac ccacatgccg
     4501 atctcgaccg gcaggttgag ctcgcgcaac gactgaccga ccgagcgcga cccttcgcgc
     4561 accttgatgc gcgagacgat ctcgtcggag tggcgaaggt cgcgcagcag gtcgtcgttg
     4621 atgccgaggc cggacgacac gacgtgcgag atgtcctccg ccgcgtcggc gatcttctcg
     4681 accgccgagg cgatctcgag gacgccggcc atgtgctccg cgtcctcggg gctgcgtgcc
     4741 gccaggatcg cgagcttccg aagctggtgg aggtggtcga acatcctgtc ctcgagcctg
     4801 cggacctcct tggcgaggtc ggcgtccgcg aagaacacgg cggcgtaggc caggtcgacc
     4861 atgagctccg acgagtcttt gatctcggcc aggagcccct tgacggttct cggaccttcc
     4921 gaatccactc gtactccccc tttggtgcgc atgtcaggct actccgaaga cgacgagtgc
     4981 gatcactagg cagatgactc ccatgaggtc catcccgctc gtgaccagcg ggatcgtgtg
     5041 gttgtcgggg tcgaacccga agcggaacgt cgtggtcgcg gcgtagtagc cgatgaccgc
     5101 cgccacgcag gtcgccagca cccctccgga gatcacgacc gcgacgaact tgaacacccc
     5161 cggatagccg gcccccgtga cctccgcgag ccccagcgtc gccaccgcgg tcacgacgta
     5221 gaccgtgatg ccgagggcga ggacgatcat cccgtcgagc agcgcggcgg ggtcggggcg
     5281 gcgggtcggg gtcacgacgc cgaggtggag cttcgacccg agccgcgccg cgaggatcgc
     5341 accgagcgcg cccgtgttct cgaggaagcc gggaacgatg atcaagaacg cggagaactt
     5401 caggaagacc tcgtcgacgc gcggctcgac cactgcgccg gcgagcacgt cgaggacgat
     5461 cgcgatgcac agcacgggga agctctcgcg gacgatccgg cgcgcgaccg agcgcgacga
     5521 cgtccatccg aggaacagcg acgcgccccc cgcgacgagc gctgcgatcc cgatcgtcac
     5581 ggtgacgacg cggacgtgga cgaggtacga cgcgagcagc aggcacggaa gcgtgaccac
     5641 gtcgccgatc gcggtgatca acggtgcgcc gaccgagtcg aggtcccatc cgcgccggta
     5701 cgacgtgatc gacacgtaga tcgtcacggc gagcacgacg acagacgaca gcaacccacc
     5761 gacgagcgcg acgacgatga agtcgagggc cgagaccgtc tcgatgccga ggagccccgc
     5821 gatcgcgcgc gcgagcagcc ccatcgtcac ggaggtcgcg accgtcagca gcgtcgcggc
     5881 gtagacgttc tgcacgagcg cgccctcgcg cgcgcgcgac acctcgaaca gaccggtgtg
     5941 gatcgacgtc ccgaggcgcg ccgcgagcgc gccgaagatg ttcccgcgca tcccgatcga
     6001 gaccgggatc agcacgaaca gcccgtcgac ctcgtcgatg cggtggctca tgcccgcgag
     6061 cgtcagacct gcgacgagcg aggtcaggct ggcgatg
//
LOCUS       DASQDO010000077         6270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_620886, whole genome shotgun sequence.
ACCESSION   DASQDO010000077 DASQDO010000000
VERSION     DASQDO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620886"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1500)
                     /locus_tag="VEV43_02400"
     CDS             complement(25..1500)
                     /locus_tag="VEV43_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017836206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22399.1"
                     /translation="MSTTVTNQNEGAVDFRAHEGRRSSQAVGKAVFAEAAGSVDASLA
                     ASIERQKDWRKTYLSPVRKLVEAGAKSGKDALRIASEGLDAVHRNLVFAQGETETTLL
                     DAVAGGGPETFDTGTVTGKGTRGREVVVPYRGTELRGDSLRRQLDTWVSAGVIERSCA
                     QAVTSVVENPEWLDLSGLSFVLLGAAAEMGPLEALTKWGADVVAVDLDRPAVWDRVLG
                     FATAGAGTLRFPEERGRAGADLLSQTPRIGAWLRSVEGPLVVGTYAYADGSTFLKLAG
                     ALDALTVSLQRGREDVSIAYLATPTDVFAVPAEVVERSRSRPASPFGRAARGLTRSKV
                     FAANYPSTVEGEGGRKWGLADALVPQQGPNYALAKMMQRWRAITAREEGTLASANVAP
                     ATRTRSVTKNKILAAAYRGAHAFGVEVFEPETSRALMAALLVHDLRSPKAAARPETPL
                     DHPYDLFVDGAAHGGMWTAAYAPRTVLPLAAALGAVKRKES"
     gene            1705..1875
                     /locus_tag="VEV43_02405"
     CDS             1705..1875
                     /locus_tag="VEV43_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22400.1"
                     /translation="MKNKRMRVATTLVAAVAAVTMVAPTPAHAFPCNDKFTQEKCQDI
                     QNLICYLFGKCL"
     gene            2069..3709
                     /locus_tag="VEV43_02410"
     CDS             2069..3709
                     /locus_tag="VEV43_02410"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HYP22401.1"
                     /translation="MAAAQDFDPRFDPHDSEVEASFLGAWFARLGALALLVGAGFGFK
                     YGMDEGFIGPGTRVLLGILAGFGLIAWGEWSERHGWPRLTQAVSGGGIAMLYLSIWAA
                     FQLYGIVTGIEAFAGLTAVAAVGGYLALRHDSCALAVLSTVGGFGNALLIGEGFDRPS
                     ALFGYVVILDCVVLGLAYVRGWRVLDHIAFGATWLFLLVGGLTATVFAELGGDESPTG
                     LVLAYATVYFFMFSALTVARRVGRSDGSQASSVFLMLANCGIYVMTAMALLAEDGTAW
                     ATADALRGPVILVAAGVNLVAGLVIRGRDADDPVAGAALASAALLLSVWVPVEIEAFA
                     VPAAWAVLGAGMIQVADRSTLTTARIPGYLLLGLSLVYLVGLFGTPGFYEPARVLVSP
                     ESAAFVVHISALYVAAFGLRNVSREDERLLFGALAVVASMMTLVWLSLEAVAYFGPFD
                     GGRAGQSLHFALNAIWGLYAAALLGVGIAARVAGARFLALGIFGVTAAKLVLHDVWML
                     DTLYRTVVFMGLGAIFLGCSVMYHRFRDLVLEDRARAA"
     gene            3880..4734
                     /locus_tag="VEV43_02415"
     CDS             3880..4734
                     /locus_tag="VEV43_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP22402.1"
                     /translation="MSTVAPAVVPAVPSNDPTPAVEVSNLRKEFYRRDRRKGRFKRRR
                     VPALHDVSFEIARGECVAILGQNGSGKSTLVRLLSTLLLADGGTARVFGHDVFDDPRP
                     IRRLVNRVSVEASFFKKMSAVENLNYAARFYGMTPSQTRPQVPRILDHVGFPSSRRTE
                     PMENLSRGMQQKVALARALLTSPVLLLLDEPTTGLDPRSKLEVQDFIRDVRAAHDATI
                     LLCTHDLAEAEALADRVGILHAGRLLALEPADRLKARYGSASLEEAFFAATGKKLAEE
                     ESAPEDDE"
     gene            4731..5564
                     /locus_tag="VEV43_02420"
     CDS             4731..5564
                     /locus_tag="VEV43_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP22403.1"
                     /translation="MRGAASTLRAELVGLGGVVERNAYLIKRYALWEVAFFFWTVANT
                     LTIVFIARGVEASGGQLDVNRQTAILLIGGVIWAYLGIIFEILTETVAWERWEGTIEY
                     TFMAPLSRATHLSGMGVFAVVYGVLRAALLFGVIAFFFDLRFPDADFVAALVVLAVAS
                     ISFIGIGMMTAVLPLISPEKGSQLGFIAQGMLLVVSGVYYSVEVLPEPMQWLATISPA
                     TYALEGIRAAVLDGAGLGSLGAELVALTIIGIVSIPLGIWVFRRGELYAKRHGKLKRS
                     G"
     gene            complement(5561..>6270)
                     /locus_tag="VEV43_02425"
     CDS             complement(5561..>6270)
                     /locus_tag="VEV43_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP22404.1"
                     /translation="RATAGECNVPFVNVRIEDVLDPYMGMSERKLHSAFQFARASAPC
                     VLFLDEIDGLGFARSRHVSATSRTLVDLLLQELDAIGAENDDLLILAATNAPWDVDEA
                     LLRPGRFDRRIFVPPPDEEARRHIFEVVSERVPNRSLDARGLAKRTPLYSGADIRALI
                     ERATDAVIDEALDGGGEPPLETRHLEAVMPAIHPTTLEWLERARHYVEFANQGGRYSE
                     IETYLKRRDVQRQLGKR"
BASE COUNT          830 a         2171 c         2216 g         1053 t
ORIGIN      
        1 gggctccggc gggtcccgcg gcggttagct ctccttccgc ttgacggccc ccagcgccgc
       61 agccaggggc aggaccgtcc gaggcgcgta ggccgccgtc cacatgcctc cgtgcgcggc
      121 cccgtccacg aacaggtcgt acgggtggtc gagcggcgtc tcgggccggg ccgccgcctt
      181 cggcgaacgc aggtcgtgaa cgagcagcgc ggccatcagc gcgcggctgg tctcgggctc
      241 gaagacctcg acaccgaacg catgcgcacc gcggtaggcc gcggcgagga tcttgttctt
      301 cgtcaccgag cgcgtgcgcg tcgcgggggc gacgttcgcg gacgcgagcg tcccctcctc
      361 acgggccgtg atcgcacgcc agcgctgcat catcttcgcc agcgcgtagt tggggccctg
      421 ctgcgggacc agggcgtcgg cgaggcccca cttcctcccg ccttcaccct cgacggtcga
      481 cggatagttc gcggcgaaca ccttggagcg ggtcagccct cgcgcggcgc ggccgaacgg
      541 agacgccggt cgcgaccgag atcgctcgac gacctcggcc gggaccgcga agacgtcggt
      601 gggcgtcgcg aggtacgcga tcgagacgtc ctcgcgtccc cgctgcaagg agaccgtgag
      661 cgcgtcgagc gcgccggcga gcttgaggaa cgtcgagccg tccgcatacg cgtacgtgcc
      721 gacgacgagc gggccctcga cggaccgcag ccacgcgcct atccgaggcg tctgcgacaa
      781 caggtccgct ccggccctgc cgcgctcctc ggggaagcgg agagtgccgg cgcccgcggt
      841 ggcaaagccg aggacccggt cccacaccgc ggggcggtcg aggtcgaccg ccacgacgtc
      901 cgccccccac ttcgtaagcg cctccagcgg gcccatctcg gcggccgctc ccagtaggac
      961 gaagctgaga cccgagaggt cgagccattc cggattctcg acgaccgacg tgacggcctg
     1021 cgcgcacgag cgctcgatca cgcccgcgct cacccacgtg tcgagctgac ggcgcagcga
     1081 gtctccgcgc agctccgtgc cccgataggg gacgacgacc tcacggcccc gcgtcccctt
     1141 cccggtcacc gttccggtgt cgaaggtctc gggcccgccg ccggctacgg cgtcgagcag
     1201 cgtggtctcg gtctcgccct gtgcgaacac gaggttgcgg tggacggcgt cgaggccctc
     1261 cgaggcgatc ctcagcgcgt ccttgcccga cttcgccccg gcctcgacca gcttgcgcac
     1321 cggcgacagg taggtcttgc gccagtcctt ctgtcgctcg atcgatgcgg cgagcgacgc
     1381 gtcgaccgac cccgcggcct cggcgaagac ggctttgccg acggcctgcg agctacgccg
     1441 gccctcgtgc gcgcggaagt cgaccgctcc ctcgttctgg ttggtaaccg tcgtgctcac
     1501 ccctcctgta tgcctcggcc gccgagcatg gcaacggggg ccggggcggt caaatgcccg
     1561 gcgtcgatcc ggccgcgctc gaaggccgtc cgggtcgcga aaacttctgg ccgccggcgg
     1621 ccatctccgg ggagggaacg gcgcccttgc ctagaaggga cccggcagtt cgactcgctg
     1681 ttgaaccgga cggaaggaga cgaagtgaag aacaagcgca tgcgggtggc gacgactctg
     1741 gtggcggccg tggccgcggt gacgatggtc gcgccgacgc cggcccacgc gttcccgtgc
     1801 aacgacaagt tcactcagga gaagtgccag gacatccaga acctcatctg ttacctgttc
     1861 gggaagtgcc tctagccgaa acgcctggct gagaaaggcg cggggtcgcg ggccccgcgc
     1921 cttttctttt tccgttccgc cgtgccggaa gtacccagga aacctgagca attcccgccc
     1981 ccggtttggt gacgagggcg gatagtcctg cagagccccc tggccgaagg tgagaccacg
     2041 agcccctacg gcacaggagg taacggacat ggctgcagct caggacttcg atccgcgctt
     2101 cgacccgcac gactcggagg tcgaggcgag cttcctcggc gcgtggttcg cgcgcctcgg
     2161 cgcgctggcc ctgctcgtcg gcgccggctt cggcttcaag tacggcatgg acgaagggtt
     2221 catcggaccg gggacccgcg tgctcctcgg catcctcgcg ggcttcggcc tgatcgcgtg
     2281 gggtgagtgg tccgagcggc acggctggcc gcgcctcacc caggcggtca gcggaggcgg
     2341 catcgcaatg ctgtacctgt cgatctgggc cgcgttccag ctgtacggga tcgtgaccgg
     2401 gatcgaagcg ttcgccgggc tcaccgcagt cgccgccgtc ggcggatacc tcgctttgag
     2461 acacgactcc tgcgcgctcg cggtgctctc gaccgtcggg ggcttcggca acgcgctgct
     2521 catcggcgag ggcttcgaca ggccgtccgc gctgttcggc tacgtcgtga tcctcgactg
     2581 cgtcgtcctc ggcctcgcgt acgtccgggg gtggcgtgtg ctggatcaca tcgcgttcgg
     2641 cgccacctgg ctcttcctgt tggtcggcgg gctgacggcg acggtcttcg ccgagctcgg
     2701 tggtgacgag tcaccgaccg gcctcgtgct cgcgtacgca accgtctact tcttcatgtt
     2761 ctccgcgctg accgtcgccc gccgggtggg acgcagcgac ggctcgcagg cttcgtcggt
     2821 cttcctcatg ctcgccaact gcgggatcta cgtcatgacg gcgatggcgc tgctggcgga
     2881 ggacgggacc gcatgggcga cggcagacgc gctgcgcggc ccggtgatcc tcgtcgcggc
     2941 cggcgtgaac ctcgtcgcgg gcctcgtcat ccggggccgc gacgccgacg accccgtcgc
     3001 gggggccgcg cttgcgtcgg ccgcgctgct cctcagcgtg tgggtgcccg tcgagatcga
     3061 ggcgttcgcg gtccccgcgg cgtgggccgt gctgggggcc gggatgatcc aggtcgccga
     3121 ccgttcgacg ctcacgacgg cgcgcatccc tgggtatctc ctgctgggcc tctcgctcgt
     3181 gtacctcgtg gggctgttcg ggacgccggg gttctacgag cccgcgcggg tgctggtctc
     3241 gccggagtcc gctgcgttcg tcgtccacat ctcggcgctc tacgtcgcgg ccttcgggtt
     3301 gcgcaacgtc tcccgcgagg acgagcgcct gctgttcgga gcgctggcgg tcgtcgcctc
     3361 gatgatgacg ctcgtgtggc tgagcctgga ggcggtcgcg tacttcggtc ccttcgacgg
     3421 gggccgcgcg gggcagtcgc tccacttcgc gctgaacgcg atctggggcc tgtacgcggc
     3481 ggcgttgctc ggcgtcggga tcgcggcccg ggtcgccgga gcgaggttcc tcgcgctcgg
     3541 catcttcggg gtgacggcgg caaagctcgt gctgcacgac gtgtggatgc tcgacacgct
     3601 ttaccgcacg gtcgtcttca tggggctcgg tgcgatcttc ctcggctgct cggtcatgta
     3661 ccaccgattc cgtgacctgg tcctcgagga cagggcccgg gccgcctagc tgcccgccgg
     3721 gaaggggccc tcgctcgtaa gcggagcgag ggccccttcc gcgcgcatac actggggctc
     3781 tgatgttgct cctcggctga cctcccgccc ccgcgcctcg tggacctccc acgggcgcct
     3841 tccttcacgc ggtccgtcga tctcccgagg agtcacccca tgtccaccgt tgcacccgcc
     3901 gtcgtccctg ccgtcccttc gaacgacccc acgccggccg tagaggtctc gaacctgcgc
     3961 aaggagttct accggcgcga ccgtcgtaag ggacggttca aacgccgtcg cgttcctgcc
     4021 ctgcacgacg tctcgttcga gatagcccgc ggtgaatgcg tcgcgatcct cggtcagaac
     4081 gggtcgggca agtcgacgct cgtccggttg ctgtcgacgc tcttgctcgc cgacgggggg
     4141 accgctcgtg tcttcgggca cgacgtgttc gacgacccgc ggccgatccg gcgtctggtg
     4201 aaccgggtct cggtcgaggc gtcgttcttc aagaagatgt cggcggtcga gaacctcaac
     4261 tacgcggccc ggttctacgg gatgacgccg tcgcagacgc gcccgcaggt gccgcgcatc
     4321 ctcgaccacg tcggcttccc gtcgagccgg cgcaccgagc cgatggagaa cctgtcgcgc
     4381 gggatgcagc agaaggtcgc gctcgcgcgg gcgctgctga cgtcgccggt cctgctgctg
     4441 ctcgacgagc cgacgaccgg gctcgacccg cgctcgaagc tcgaggtcca ggacttcatc
     4501 cgggacgttc gtgccgcgca cgacgcgacg atccttctgt gcacgcacga cctcgccgag
     4561 gcagaggccc tcgccgatcg cgtcgggata ctccacgccg ggaggcttct cgcgctcgag
     4621 ccggcggacc ggctgaaggc gcgctacggc tcggcctcgc tggaggaggc gttcttcgcc
     4681 gccaccggca agaagctggc cgaagaggag agcgcgccgg aggacgacga atgagggggg
     4741 ccgcgtcgac gctgcgtgcc gagctcgtcg ggctcggcgg cgtcgtcgag cgaaacgcct
     4801 acctcatcaa gcgctacgcg ctgtgggagg tcgcgttctt cttctggacg gtcgcgaaca
     4861 cgctcacgat cgtgttcatc gcacgcggcg tcgaggcgtc cggcggtcag ctcgacgtca
     4921 accggcagac ggccatcctg ctcatcggcg gcgtcatctg ggcgtacctc gggatcatct
     4981 tcgagatcct gaccgagacc gtcgcgtggg agcggtggga gggcacgatc gagtacacgt
     5041 tcatggcgcc gctgtcgcgg gcgacgcacc tctctggcat gggcgtgttc gcggtcgtct
     5101 acggggtctt gcgggccgcg ctgctgttcg gcgtgatcgc gttcttcttc gacctgcggt
     5161 tcccggacgc ggacttcgtc gcggccctcg tggtgctcgc ggtcgcgtcg atctcgttca
     5221 tcggcatcgg gatgatgacc gcggtgctgc cgctgatctc gccggagaag gggtcgcagc
     5281 tcggcttcat cgcacagggg atgctgctcg tggtgtcggg cgtgtactac agcgtcgagg
     5341 tgctgccgga gccgatgcag tggctggcga cgatctcgcc cgcgacgtac gcgctggaag
     5401 gcatccgggc ggcggtgctg gacggagcgg gcctggggag cctgggggcc gagctggtgg
     5461 cgctgacgat catcgggatc gtgtcgatcc cgctggggat atgggtcttc aggaggggcg
     5521 agctatacgc gaagcgacac ggcaagctga agcgctcggg ctagcgtttg cctagctggc
     5581 gctgtacgtc gcgccgtttc aggtacgtct ctatctccga gtaccggccc ccctggttcg
     5641 cgaactccac gtagtgccgc gcccgttcca gccattcgag cgtcgtcggg tggatcgccg
     5701 gcatcacggc ttcgaggtgc cgggtctcga gcggcggctc gcctccgccg tccagggcct
     5761 cgtcgatcac cgcgtcggtc gctcgctcga tcagcgcgcg gatgtcggca ccgctgtaca
     5821 gcggggtccg tttcgctaac cctcgagcgt cgagcgaccg gttcgggacc cgttcggaca
     5881 cgacctcgaa gatgtgccgg cgcgcctcct cgtcgggcgg cgggacgaag atgcgacggt
     5941 cgaaccggcc cgggcgcagc agggcctcgt cgacgtccca cggcgcgttc gtcgcggcga
     6001 ggatcagcag gtcgtcgttc tcggcgccga tcgcatccag ctcctgcagc aggaggtcga
     6061 cgagcgtgcg cgaggtggcc gagacgtgcc ggctgcgcgc gaacccgagc ccgtcgatct
     6121 cgtcgaggaa caacacgcag ggggccgacg cccgcgcgaa ctggaacgcg gagtggagct
     6181 tgcgctcgct catccccatg taggggtcga ggacgtcttc gatgcggacg ttcacgaacg
     6241 ggacgttgca ctctccggcc gtggcccgcg
//
LOCUS       DASQDO010000078         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_926756, whole genome shotgun sequence.
ACCESSION   DASQDO010000078 DASQDO010000000
VERSION     DASQDO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926756"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /gene="ftsH"
                     /locus_tag="VEV43_02430"
     CDS             <1..1784
                     /gene="ftsH"
                     /locus_tag="VEV43_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYP22405.1"
                     /translation="SFTRLFELAEDGKITEASVSETSVSGTIEERGEDPRQFTTTIPP
                     NYEANAIVGRLEKAEVDVTGRQPSAWTAFLFAWIFPLALIGGLYYLFMRRIRKQMGGA
                     GGPLSLGKNKAKLYDRTDMKTTFADVAGVDEVEAELVELVDYLKRPGKYEKIGARIPK
                     GVLLVGPPGTGKTLLARAVAGEAGVPFFYISASEFIEMFVGLGAARVRDLFEQARGRA
                     PAIICIDEIDAIGQSRAGAAGNQLGAHQEQEQTLQQLLTEMDGFEPNSGVIIVAASNR
                     PEVLDQALLRPGRFDRQIQVNLPDIGGRREILEIHAKRVRLAPDVDLRTLARRTPGFS
                     GAQLANVINEGALLAARRSREVVDMRDLEEAIDRVVAGLERRSQVLTDNERRLVAYHE
                     LGHALVARFVEHADPVHRVSIIPRGIGALGYTQQLPDEERYLMSEPELRDRIAVLLGG
                     RSAEEIVFGYATTGAHDDLKKATEIARRMVTEFGMSEKVGPVNLAGDGARFLASAWRR
                     TDDVSEETETAIDREVKAILQEGQGKAREILNAHRHDLDVLAEVLLEKESLDRRDLDS
                     HFSPTKPVDLRERRTTAELDGPRHPLA"
     gene            complement(1798..2001)
                     /locus_tag="VEV43_02435"
     CDS             complement(1798..2001)
                     /locus_tag="VEV43_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22406.1"
                     /translation="MQYRTALVGNVSNRWTKRGAEQEMNQLTATINSFGQGSRLHSVQ
                     PIPVFNIAGNQTGITLLAVYEDD"
     gene            2231..>2828
                     /locus_tag="VEV43_02440"
     CDS             2231..>2828
                     /locus_tag="VEV43_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYP22407.1"
                     /translation="MDHEKLTIKTQQALVAAQKLAGDANHQQVTPAHVMAALLADPEG
                     VVYPLLHKLGVAPRSIAARLQGSLDRLPKVYGGSQPYLSQEVSDLLDAAAAEAETLGD
                     AYVSTEHVLLAFLDSRDEAGRALTEAGVDRARVLNALKEIRGSQRVTDQNPEETYQAL
                     ERFGRDLTDAARRGKLDPVIGRDDEIRRVIQVLSRRTKN"
BASE COUNT          480 a         1000 c          962 g          386 t
ORIGIN      
        1 tctcgttcac gcggctgttc gagctcgccg aggacggaaa gatcaccgag gccagcgtct
       61 ccgagacgag cgtctccggc acgatcgagg agcgcggcga ggacccccgc cagttcacga
      121 cgacgatccc gccgaactac gaggcgaacg cgatcgtcgg ccggctcgag aaggccgagg
      181 tcgacgtcac cggacgccag ccgagcgcgt ggaccgcgtt cctgttcgcg tggatcttcc
      241 cgctggcgct gatcggcggt ctctactacc tgttcatgcg ccgcatccgc aagcagatgg
      301 gcggtgcagg cgggccgctg tcgctcggca agaacaaggc gaagctctac gaccgcaccg
      361 acatgaagac gacgttcgcc gacgtcgcgg gcgtggacga ggtcgaagcg gagctcgtcg
      421 agctcgtcga ctacctcaag cgtcccggga agtacgagaa gatcggtgcg cgcatcccca
      481 agggcgtgct gctcgtgggc ccgcccggca ccgggaagac gctgctcgcc cgggcggtcg
      541 ccggtgaggc cggcgtcccg ttcttctaca tctccgcgtc ggagttcatc gagatgttcg
      601 tcggcctcgg cgcggctcgc gtccgcgacc tcttcgagca agcgcgcggc cgcgctcccg
      661 cgatcatctg catcgacgag atcgacgcga tcggtcagtc gcgcgcgggt gcggccggca
      721 accagctcgg cgcgcaccag gagcaggagc agaccctgca gcagctgctc accgagatgg
      781 acggcttcga gcccaacagc ggcgtgatca tcgtcgccgc gtcgaaccgg ccggaggtcc
      841 tggaccaggc cctgctccgg cccggccgct tcgaccgcca gatccaggtg aacctgcccg
      901 acatcggcgg gcgccgggaa atcctcgaga tccacgcgaa gcgggtccgc ctcgcgcccg
      961 acgtcgacct gcgcacgctc gcgcgccgca cgccgggctt ctccggggcg cagctcgcga
     1021 acgtgatcaa cgagggcgcg ctgctggcgg cgcgccgcag ccgcgaggtc gtcgacatgc
     1081 gtgacctgga ggaggcgatc gaccgtgtcg tcgccgggct cgaacggcgc tcgcaggtgc
     1141 tgacggacaa cgagcgcagg ctcgtcgcct accacgagct aggccacgcg ctcgtggccc
     1201 ggttcgtgga gcacgccgac ccggtccacc gcgtctcgat catcccgcgc gggatcggcg
     1261 cgctcggcta cacgcagcag ctccccgacg aggagcgcta cctgatgtcc gagcccgagc
     1321 tgcgcgaccg catcgcggtg ctgctcgggg gccggagcgc ggaggagatc gtgttcggct
     1381 acgcgaccac cggcgcgcac gacgacctca agaaggcgac cgagatcgcc cgccgcatgg
     1441 tgacggagtt cggcatgtcc gagaaggtcg gcccggtcaa cctcgcgggc gacggcgcgc
     1501 gcttcctggc gtccgcgtgg agacgcacgg acgacgtgag cgaggagacc gagaccgcga
     1561 tcgaccgcga ggtgaaggcg atcctgcagg agggccaggg caaggcccgc gagatcctga
     1621 acgcccaccg ccacgacctg gacgtgctgg cggaggtcct gctggagaag gagagcctgg
     1681 accgccggga cctggacagc cacttctcgc cgaccaagcc cgtagacctc cgggagcgcc
     1741 gcaccacggc cgagctggac ggcccccgcc accccctcgc gtagcggcga cgcgttctca
     1801 gtcgtcttcg tacacggcga ggagagtgat ccccgtctgg ttgcccgcga tgttgaagac
     1861 cgggatcggt tgaaccgagt ggagccgcga gccttgtccg aaggagttga tggtggccgt
     1921 cagctggttc atctcctgct ccgcccccct cttcgtccac ctgttcgaca cgttgccgac
     1981 caaagccgtc ctgtactgca tcgggcctcc tcgtccgcgg ttgccagaga cgagttcaga
     2041 gtagggcgag gcctggcgaa agcgtcggcg ggccacacgc acacaaccgt cggcgacatg
     2101 tctgacacca accgctaggg ggacgcaggg cgagagccgg cgccgggaat cccgaggcct
     2161 ctccgaaagt tgactatatg gcactcaact ttcatactag gcggatagtc atctcctgtc
     2221 ggagaaaccc atggaccacg aaaagctcac gatcaagacc cagcaggccc tcgtcgccgc
     2281 acagaagctc gccggcgacg ccaaccacca gcaggtgacg ccggcccacg tcatggccgc
     2341 gctcctcgcc gatcccgagg gcgtcgtcta cccgctgctg cacaagctcg gcgtggcgcc
     2401 ccggtcgatc gcggcgcggc tccagggatc gctcgaccgg ctcccgaagg tctacggcgg
     2461 ctcacagcct tacctgtcgc aagaggtgtc ggacctgctc gacgccgccg cagcggaggc
     2521 cgagacgctc ggcgacgcct acgtgtcgac cgagcacgtc ctgctcgcgt tcttggactc
     2581 gcgcgacgaa gcaggccgcg cgctgacgga ggccggcgtc gaccgcgccc gcgtgctgaa
     2641 cgcgctgaag gagatccgcg ggtcccagcg cgtgaccgac cagaaccccg aggagacgta
     2701 ccaggcgctc gagcgcttcg gacgcgacct caccgacgcg gcccggcgcg gcaagctcga
     2761 cccggtcatc ggccgcgacg acgagatcag gcgcgtgatc caggtcctct cgcgacgcac
     2821 gaagaaca
//
LOCUS       DASQDO010000079        12880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_865545, whole genome shotgun sequence.
ACCESSION   DASQDO010000079 DASQDO010000000
VERSION     DASQDO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865545"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..420)
                     /locus_tag="VEV43_02445"
     CDS             complement(46..420)
                     /locus_tag="VEV43_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22408.1"
                     /translation="MPPTRGILVVSDDPALQLEARFAFPDDFDVVVVEDAREGSRELS
                     AWTPAAVVVDLLVGSAGGFALGRDMSQDPRLADVPMLMLIDRRQDEWLAREAGAAAVR
                     TKPIDTAELVESTLDLVGENAK"
     gene            complement(431..721)
                     /locus_tag="VEV43_02450"
     CDS             complement(431..721)
                     /locus_tag="VEV43_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22409.1"
                     /translation="MKIEATCDTCERTFLLTQIGPDSDAPGRCPFCGARFARHYSTVL
                     AETVREAENAAQRFTSILGRLQSMETGFDIHFERMLRSLDESVRSHAQQTSG"
     gene            complement(750..2150)
                     /locus_tag="VEV43_02455"
     CDS             complement(750..2150)
                     /locus_tag="VEV43_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HYP22410.1"
                     /translation="MSTVVDDAAADWGIEPVPPGHRTLSGFDLAVLWGDLGVGLLVLV
                     TGALLVPGLGFGAAAAAIVLGSVIGVALLAAAGAAGAEHGVTSMVLFRPVLGVRGSWI
                     PSALNAVQLVGWTAVELWAMSFVADLVSQRVFGWSSRGLWLAAATVVCTALALWGPLG
                     VTRIWMKRFAAWVAGAICVLVTIAVLTTDGLSAALARPGAGGFPTFGLALDLVIAMPV
                     SWLPLVADYTRFGRRPRAAFGGTFAGYLVANVWLYLLGVLLVLGPGAEPSPAGIATGV
                     LAVAGGSLAGLLFLAGLLAGETDEAFADVYSGALSLRNIFPGLSRTAATIGIAAVSWA
                     LAAWLTMERYESFLFLIGSVFVPLFGILVADHFVKRRRRIVVEDLDREGGRYWYSRGV
                     RGAALGPWLAGFVLYHWIAPNGPAWWLAWTESLLGTPLSARWPWLSASVASFAVAFLV
                     TLTLRGPRADDDARLS"
     regulatory      complement(2143..2251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2383..3711
                     /locus_tag="VEV43_02460"
     CDS             2383..3711
                     /locus_tag="VEV43_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22411.1"
                     /translation="MRFATTRKTYLAGALALLLMVMVFPAAAGAATKRSGKQKQARDS
                     IFIDELKDFNPANGVRAGSGSASDPFVIANWDVSSIQISDTDAHVVIRDNVVDTLVLD
                     WIGGGIEVRNNDVGDLRVNQNRTRTGDPTSGVIENNKFGIVGQLRHFDGIFQNNVVGV
                     KEGMNLPFITDTRAVNFDGFNGSIFRNNTIYGYMDARLHGHHHSSGFAEQSHYHGDSP
                     AAHEDHGDMDHTVRYHQVHIYGNKIYADHYYALGYLDTNHSANDRTAASETEETLNDP
                     HIHYTKVFIHDNELVGAGLYVDVFNAKDTLHKQIGRGLMEISDNKIVLHRTKDATQRF
                     GFPRNGIDVSQAQEITLRILDNTITGPEAGDALTDLTGESEVAGINLQNFDKGDLLLS
                     GNAVTRAAYGIRAYDFTETVNWTIQELTANEVGQDVYWDSTVANAPKRRG"
     gene            3866..5197
                     /locus_tag="VEV43_02465"
     CDS             3866..5197
                     /locus_tag="VEV43_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22412.1"
                     /translation="MRGRGKRLAAGAAVALALAALPGIPAAASAAEAADRERVARDAI
                     LIQSAADFNPANGVRAGSGTKADPYVISDWEVPSLVIRNTNRWVTIQDNVVTGQMVLD
                     WIGPGVRVVRNDVADLRVNQNVERTGPATDGYIGNNTFGLVGQLRHFDGIFERNVVGT
                     EHVLAKRPSFKAVQFDGFNGARFRDNTIYGYVDVKLHGHHHGSRYGDHSHDHHATYDA
                     EGIPTHEHGSAHVDHSRRYHEVFISGNELHSDHDWALRYYDRAHTGDDRTAASETDKN
                     LNCPHVHFTRVHLTGNKLIGSGIAVDVFNASDDNHWTTERGSVEIRGNKIKIERASTE
                     LLRGAHGILVQEAVDVDVDITGNYIVGPETSGERDLLGLDKELHFGSGIKLHQLDQAR
                     VRIVDNMVLQRTYGVDATSFTGSVRWTVRNLKVAHVKEKVHYDETVSRKPS"
     gene            complement(5214..6404)
                     /locus_tag="VEV43_02470"
     CDS             complement(5214..6404)
                     /locus_tag="VEV43_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22413.1"
                     /translation="MSRTQRLGAAALATILCLSTSVAVSSRPKDRPFRATRHWEGQSV
                     AEGDYFAWQQISRGSPNRADLFLRKPSGKVVKLNRPGTQGTLGAIDGDSLVFQEFRGQ
                     PAVFGGNGSSRIVLYDLKTGKKTLPPVANGSEWEYWPTIRGPWIFFAQLDHFGSRHVI
                     GYHRPSGAVTRADSYVSYLQPGQINAGLFAWVRQEPGAGPASVRVLDLESSSYDELRS
                     RRWQWAPSVGPQGAVYVVETGKKCGSSPVIKRYPPALEGGIDPVGKEILRLPEGFDSS
                     SSYAFTNPQGHTTVLHQRLKCGTKWGSDVYRFTDTVGLTVTKDGSGSGSVTGVDVAID
                     CGDDCTEVVSGGTWVTLDADAELGSHFAGWSEQCAVSNEDEDTCTIRLDDALTINATF
                     DLGP"
     gene            6540..7661
                     /locus_tag="VEV43_02475"
     CDS             6540..7661
                     /locus_tag="VEV43_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22414.1"
                     /translation="MRRTRTILALAALLAASVPAPPARAVGLTNGPIEYVKTIPIDAG
                     AAQVAKRVGDHLYLTSWKALSIYDVTDLLNPVHVSTIPTGFQFPAERMDTNGEILIIG
                     EQTPVYRLHVFDVSDPADPSEIATLAGTPDHTFTCVLDCRWAYGSHGTIVDLRDPANP
                     AVAGDWSEPSLSYWHDVTEVAPGRVLTASMPTMRLLDGRERVTKPRHVAMGDATSSGT
                     TGYLGTVLWPREGKDRFILGSEETSFNPQCSDTTSAFVTWDASRWRKTRTFAVADHFR
                     LGNGTLADGSPPASVVGCSTHVFDEHPGFRDGGLVALAHYEHGTRILEIGRDGSIEEA
                     AWFLPAGGSTSASYWMSDEIVYSIDFHRGLDILRYTGAA"
     gene            complement(7734..8108)
                     /locus_tag="VEV43_02480"
     CDS             complement(7734..8108)
                     /locus_tag="VEV43_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018024577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HYP22415.1"
                     /translation="MSTQELTQSEIGITPSAASKVTELLEREVSASPEEHAGKEYALR
                     IAVQPGGCAGLRYALYFDDRKLDDDKVVTAHGVEIRVDKMSAPYLAGAEVDYVDGLQQ
                     SGFTINNPNAKGTCACGDSAQF"
     gene            complement(8172..8372)
                     /locus_tag="VEV43_02485"
     CDS             complement(8172..8372)
                     /locus_tag="VEV43_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22416.1"
                     /translation="MTSWHEVWHVTVLAASLFGGAAVALVLLAPLVFETEPEGLARAR
                     PYLLGLGAFAGLLLAVEWLGVH"
     gene            complement(8369..9727)
                     /locus_tag="VEV43_02490"
     CDS             complement(8369..9727)
                     /locus_tag="VEV43_02490"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HYP22417.1"
                     /translation="MSGLRLGFDDARAAAEAVLGLPGADGVEVTVHASASGVTRYANS
                     QIIQATERDELRVYVRVVTEGRLATATTNQLSPERLEGAAQHALEAARSSLPDSGFTG
                     LADPEVVGRAEAIERVDAPTVAASATDRAAAVKAILGAAGGGNAAGVVETGVQAYGVF
                     NSHGIACFDSYTRAVATALVDDGEATGWGECSSHAIGDLDWPAVGVLAAEKAAAGHDA
                     GDAEPGDYEVVLEAPAVATMLDYLSYVGFGAKQVIDGESFLSTRTGDEVAAPSVTIAD
                     DAWHPRSVGIGFDFEGVPRRRVDVIEDGVAREPVTDLRTAAQMEVTPSGHYSGSNEWG
                     PYASNVVLEVGDQSYEDLVGAVEDGLLVTRFHYVNVLDRPSTLLTGMTRDGTFRIRDG
                     RIAEPVRNLRFSQSVLDTLSSVLGVGRDLHAFAPDFASFGSTVAPAIRVGRFHFSSGT
                     TH"
     gene            complement(9724..11169)
                     /locus_tag="VEV43_02495"
     CDS             complement(9724..11169)
                     /locus_tag="VEV43_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HYP22418.1"
                     /translation="MEDLLEAALDAAVAAGASYADVRSVESASEALSVRGDVVEALER
                     SDSEGFGVRVLVDGAWGFAASYDLEQGSAASTARAAVEVARATATALESPVELVPEPP
                     HRATWSSPLEVDPFSVSLEDKVGLLASATRAMSVSPAIRAARGTMDVLKQRTFFVSSE
                     GAAIDQTIIHTGAGIEATAVEGSEVQTRSYPGSFRGHLEAGGYEIVTAMDLEAHAQQT
                     GEEAVALLSAPDCPAGDTTVVLDGHQVMLQVHESVGHPTELDRVLGMEAAFAGTSFVG
                     IPDLGSLRYGSELVSIVSDATTPGGLGTFGYDDEGVEAQCVDLVRDGVLVGFQTSRET
                     AAAAGADRSNGTMRAEGWENFPLIRMTNVNLLPGAGSFEDLLADVDDGIYMATNKSWS
                     IDDKRKNFQFGCEIAWEIKNGRLGRMLRNPRYTGITPVFWAGCDAIAGPDEWRIWGTP
                     NCGKGQPGQTMRVGHGTAPARFRNVSTGTSQ"
     gene            complement(11169..12791)
                     /locus_tag="VEV43_02500"
     CDS             complement(11169..12791)
                     /locus_tag="VEV43_02500"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HYP22419.1"
                     /translation="MPRLTEQLHGGRAVTRALRALGVEHLFTLTGGHIFPILDGAHQD
                     GIRIVDTRHEQSAAFAAEGWSRLTRSLGVCAVTAGPGVTNALSPLAQAGFNGAPVLCL
                     AGRAPGFRWGQGSLQEIDHVPIVEPLAKARTVASTEDVAGDVFAAALAALTPPRGPQF
                     LDFPLEVLFNNWSGGDLTAPEIPAPAAPDVDEVARLLSEAERPVLIAGSNVWLDGAET
                     ALRDLVETAELPTFTNGQGRGCLPADHRLCFSRSRNRAFKEADLVVVAGTSMDFRLAF
                     GQGFAPGAKVVHLDSHADLIAQHVDLAAGVGANLKASFEAIAAALSSPPDTKAWAEAL
                     RETEDAKRDAAQADLRSSSHPIHPQRVYGDLVPLLDRDAVVICDGGDFASFAGREIPS
                     YEGGCWLDTGPFGCLGVGPGYAMAARLAHPDRQVVVMYGDGAIGFSGMELETLVRLKL
                     PVVCVVGNNGIWALEKYPMQALYGYDVAAELRPGIRYDKVMEAFGGRGFYVEDPDDLT
                     PALKAAFDSGEPAVVNVITDSSVAYPRSSNLA"
BASE COUNT         2122 a         4642 c         4363 g         1753 t
ORIGIN      
        1 gtgattctaa gcatgtaatt tacccggtgc aataggcctg acctgctact ttgcgttctc
       61 ccccaccaga tcgagggtgc tctcgacgag ctcggccgtg tcgatcggct tggttcggac
      121 cgcggcggcg cccgcttccc gcgcgagcca ctcgtcctgc cggcggtcga tcagcatgag
      181 catcgggacg tccgcgagac gcgggtcctg cgacatgtcg cggccgagcg cgaaacctcc
      241 cgcgctcccc accaggaggt cgacgacgac ggcggccgga gtccacgccg acagctcgcg
      301 tgagccctcg cgcgcgtctt cgactacgac gacgtcgaag tcgtcgggga acgcgaagcg
      361 cgcctcgagc tgcagcgccg ggtcgtcgct gaccacgagg atcccgcgtg tgggcggcat
      421 gtgcgccggg tcagccggag gtctgctgcg cgtgcgagcg gacggattcg tcgagcgacc
      481 gcagcatgcg ttcgaagtgg atgtcgaagc cggtctccat cgactggaga cgcccgagga
      541 tcgaggtgaa gcgctgggcc gcgttctcgg cctcgcgcac ggtctcggcg agaacggttg
      601 aataatgccg cgcgaagcga gctccgcaga aggggcagcg gccgggcgcg tcggagtcgg
      661 gaccgatctg cgtgagcagg aacgtccgct cgcacgtgtc gcacgtcgct tcgatcttca
      721 tgagctccct ctcgtcgggt ctgtgcggtt catgatagtc gagcgtcgtc gtccgctcgg
      781 gggccgcgca aggtcaacgt cacgaggaac gcgaccgcga acgaagccac cgacgcgctg
      841 agccacggcc atcgcgccga cagaggcgta cccaggagcg actcggtcca cgccagccac
      901 cacgccgggc cgttcggtgc gatccagtgg tagaggacga acccggcgag ccacggcccc
      961 agcgcggccc ccctgacccc acgcgagtac cagtaacgtc ccccctcacg gtcgaggtcc
     1021 tcgacgacga tccgccggcg ccgtttcacg aagtggtcgg cgacgaggat gccgaacagc
     1081 gggacgaaga ccgagccgat caggaacagg aagctctcgt accgctccat cgtcagccac
     1141 gctgcgaggg cccacgacac cgcggcgatg ccgatcgtcg cggccgtccg cgacagcccc
     1201 gggaagatgt tgcgcaagct gagcgctccg gagtacacgt ccgcgaacgc ctcgtcggtc
     1261 tcgcccgcga ggagccccgc gaggaacagc agcccggcga gcgatccacc cgcgaccgcg
     1321 agcacgccgg tcgcgatccc ggctggcgag ggctcggccc ccgggccgag cacgagcagc
     1381 acgccgagca gatagagcca cacgttcgcg acgaggtatc ccgcgaacgt cccgccgaac
     1441 gcggcgcgcg gcctcctgcc gaagcgcgtg tagtcggcga cgagcggcag ccacgacacc
     1501 ggcatcgcga tcacgaggtc gagcgcgagg ccgaacgtgg ggaagccgcc cgcgccgggc
     1561 cgcgccaaag cggcggacag cccgtcggtc gtgagcaccg cgatcgtcac gaggacgcag
     1621 atcgcgcccg cgacccacgc ggcgaacctc ttcatccaga tccgcgtcac gcccagcggt
     1681 ccccacagcg cgagcgcggt gcacaccacg gtcgcggccg cgagccacag cccccgggac
     1741 gaccatccga agacgcgctg cgagacgagg tcggcgacga acgacatcgc ccacagctcg
     1801 accgccgtcc acccgacgag ctggaccgcg ttcagggcgc tggggatcca cgacccccgc
     1861 acgccgagga cgggccggaa cagcaccatc gaggtgacgc cgtgctcggc ccccgcggct
     1921 ccggcggcgg ccagcagagc gacgccgatc accgacccga ggacgatcgc ggccgcggcc
     1981 gcgccgaacc cgaggccggg gacgagcagg gcccccgtca cgaggacgag gagcccgacg
     2041 ccgagatcgc cccacaggac cgctaggtcg aaccccgaga gcgtccggtg gccggggggg
     2101 acgggctcga tcccccagtc ggcggccgcg tcgtccacga cggtactcat ttattgcgtc
     2161 tcccttcgca ggtgcgagcc tgatcaggtt cgaggggtcg gccgaagccc tctcagccgg
     2221 ccatccgtgg ccggctcccc cgcgctgggc acaccttagt gcccttgggg agacgtttcc
     2281 cccggctcgg ttacctcctc cggccaccgg cgtagacgga gataggacaa cgccccaaac
     2341 cacctcgaat ggggcaggcg gccaaggaga aggaggaagc tgatgcggtt cgctacgacc
     2401 cgcaagacgt acctcgcagg agcccttgcg ctcctgctga tggtgatggt gttcccggct
     2461 gcggcgggcg cggccacgaa gcggtcgggc aagcagaagc aggcccgcga cagcatcttc
     2521 atcgacgagc tgaaggactt caacccggcg aacggcgtac gcgccggctc gggctcggcc
     2581 tccgacccgt tcgtgatcgc gaactgggac gtctcctcca tccagatcag cgacaccgac
     2641 gcccacgtcg tcatccggga caacgtcgtc gacaccctcg tcctcgactg gatcggcggc
     2701 ggcatcgagg tccgcaacaa cgacgtcggc gacctccgcg tgaaccagaa ccgcacgcgc
     2761 accggggacc cgacgagcgg cgtcatcgag aacaacaagt tcgggatcgt cggccagctc
     2821 cgccacttcg acggcatctt ccagaacaac gtcgtcggcg tgaaggaggg catgaacctc
     2881 cccttcatca ccgacacgcg cgcggtgaac ttcgacggct tcaacgggtc gatcttccgt
     2941 aacaacacga tctacggcta catggacgcc cgcctccacg ggcaccacca cagctcgggc
     3001 ttcgccgagc agagccacta ccacggtgac tcccccgccg cccacgagga ccacggcgac
     3061 atggaccaca cggtccgcta ccaccaggtc cacatctacg gcaacaagat ctacgccgac
     3121 cactactacg cgctggggta cctcgacacg aaccactcgg ccaacgaccg cacggcggcg
     3181 tccgagaccg aggagaccct caacgacccg cacatccact acacgaaggt cttcatccac
     3241 gacaacgagc tcgtcggcgc cggcctgtac gtcgacgtct ttaacgccaa ggacaccctg
     3301 cacaagcaga tcggccgggg cctgatggag atcagcgaca acaagatcgt cctgcaccgg
     3361 accaaggacg cgacgcagcg gttcgggttc ccgcgcaacg ggatcgacgt ctcgcaggct
     3421 caggagatca cgctgcggat cctcgacaac acgatcaccg gccccgaggc gggagacgcg
     3481 ttgaccgacc tcacgggtga gtcggaggtc gccgggatca acctccagaa cttcgacaag
     3541 ggcgaccttc tcctgtcggg caacgccgtc acccgggccg cgtacgggat cagggcctac
     3601 gacttcacgg agaccgtcaa ctggacgatc caggagctca cggcgaacga ggtcggccag
     3661 gacgtctact gggactcgac ggtcgcgaac gctccgaagc gccgggggta gcggccggca
     3721 gcccccgacc tccgttaccc gggaggagca ggggcgtaga cgaagaagaa gagcaagagg
     3781 cccggtcccc aatccccgga ccgggcctct tgctacccgt cgttcgacgg gggcagcggc
     3841 gggctcgggc acgaggaggc gaactatgcg tggacgaggc aagaggctgg cggccggcgc
     3901 ggcggtggct ctggcactgg cggcccttcc cgggatcccg gcggccgcgt cggccgcgga
     3961 ggcggccgac cgcgagcggg tggcccgcga cgcgatcctg atccagtccg cggccgactt
     4021 caacccggcg aacggcgtgc gcgccggcag cggcaccaag gccgacccct acgtgatctc
     4081 cgactgggag gtcccgtcgc tggtgatccg caacacgaac cggtgggtga cgatccagga
     4141 caacgtcgtg accggccaga tggtcctcga ctggatcggg ccgggcgtcc gcgtcgtccg
     4201 caacgacgtc gccgacctcc gcgtgaacca gaacgtcgag cggacggggc cggcgaccga
     4261 cgggtacatc ggcaacaaca cgttcgggct cgtcggccag ctgcgtcact tcgacgggat
     4321 cttcgagcgc aacgtcgtgg ggaccgagca cgtgctcgcg aaacggccct cgttcaaggc
     4381 ggtccagttc gacgggttca acggggccag gttccgcgac aacacgatct acggctacgt
     4441 ggacgtgaag ctccacggcc accaccacgg gtcgcgctac ggcgaccaca gccacgacca
     4501 ccacgcgacg tacgacgccg aggggatccc gacccacgag cacgggagcg cgcacgtcga
     4561 ccactcacgc cggtaccacg aggtcttcat ctcgggcaac gagctccact ccgaccacga
     4621 ctgggcgctg cgctactacg accgcgccca cacgggcgac gaccggacgg ccgcgtcgga
     4681 gaccgacaag aacctcaact gcccccacgt ccacttcacg cgggtccacc tgacgggcaa
     4741 caagctgatc ggctccggca tcgcggtcga cgtcttcaac gcgtcggacg acaaccactg
     4801 gacgacagag cgcgggagcg tcgagatccg cgggaacaag atcaagatcg agcgcgcctc
     4861 gaccgagctg ctgcgggggg cccacggcat cctcgtccag gaggcggtgg acgtcgacgt
     4921 cgacatcacc ggcaactaca tcgtcgggcc cgagacctcc ggcgaacgcg acctgctcgg
     4981 cctcgacaag gagctccact tcgggtcggg gatcaagctc caccagctcg accaggcccg
     5041 cgtgcggatc gtcgacaaca tggtgctgca gcggacgtac ggggtcgacg cgacgagctt
     5101 cacggggtcc gtccgctgga cggtccgcaa cctgaaggtc gcccacgtca aggagaaggt
     5161 ccactacgac gagacggtgt cgcgcaagcc cagctgatcc cggcgacccc cggtcagggg
     5221 ccgaggtcga acgtggcgtt gatcgtcagc gcgtcgtcga gcctgatcgt gcaggtgtcc
     5281 tcgtcttcgt tcgagaccgc gcactgctcg ctccacccgg cgaagtgcga gccgagctcc
     5341 gcgtcggcgt cgagcgtcac ccaggtcccg ccgctgacga cctcggtgca gtcgtcgcca
     5401 cagtcgatcg cgacgtccac gccggtcacg ctgcccgatc cggacccgtc cttcgtgacg
     5461 gtcagcccga cggtgtcggt gaaccggtag acgtcgctgc cccacttggt gccgcacttc
     5521 aggcgctggt gcaggacggt ggtgtggccc tgcgggttcg tgaacgcata gctcgaggac
     5581 gagtcgaagc cctcggggag gcgcaggatc tccttgccga cggggtcgat accgccttcg
     5641 agcgcgggcg gatagcgctt gatcaccggt gaggacccgc acttcttgcc ggtctcgacg
     5701 acatacacgg caccctgggg acccaccgag ggggcccatt gccagcggcg cgagcgcagc
     5761 tcgtcgtagc tcgaggactc gaggtcgagg acacggacgc tcgccggtcc cgcgccgggc
     5821 tcctggcgga cccaggcgaa caacccggcg ttgatctgtc ccggttgcag gtaggagacg
     5881 tagctgtcgg ccctggtgac cgcgcccgag gggcggtggt agccgatgac gtgcctgctg
     5941 ccgaagtggt cgagctgagc gaagaagatc cacggccccc tgatcgtcgg ccagtactcc
     6001 cactcgctgc cgttcgccac cggcgggagc gtcttcttgc ccgtcttcag gtcgtagagc
     6061 acgatccgcg aggagccgtt ccccccgaac accgccggct ggccgcggaa ctcctggaag
     6121 acgagcgagt cgccgtcgat cgcacccagc gtcccttgcg tcccggggcg gttcagcttc
     6181 acgaccttgc cggacggttt ccgcaggaag aggtcggcgc ggttgggtga gccccgcgag
     6241 atctgctgcc acgcgaagta gtcgccctcg gcgacggact gaccctccca gtgccgcgtg
     6301 gcgcggaagg ggcggtcctt cggacgcgac gagaccgcca cggacgtgga cagacacaag
     6361 atcgtcgcga gcgcagccgc gcccagcctt tgcgttcggc tcatcatccc cctcggggat
     6421 acgagaacag atacgggcac cggctacaag ggggtcccgc ccgttcgggc cgccggtttg
     6481 cgctttctgg cccgggtcgg tggaagggga acgagggcgg gcgatccaat cggtccgcca
     6541 tgagacgaac ccgcacgatc ctcgccctcg ccgcgctcct cgccgcctcg gtgccggccc
     6601 cgccggcccg cgccgtaggc ctcacgaacg gcccgatcga gtacgtgaag acgatcccga
     6661 tcgacgcggg ggccgcgcag gtggcgaagc gcgtcggcga ccacctctac ctcacgtcgt
     6721 ggaaggcgct gtcgatctac gacgtcacgg accttctcaa cccggtgcac gtgagcacga
     6781 tcccgaccgg cttccagttc cccgccgagc gcatggacac gaacggcgag atcctgataa
     6841 tcggcgagca gacgcccgtc taccggctgc acgtgttcga cgtgagcgac cccgcggacc
     6901 cgtccgagat cgcgacgctc gccggcaccc ccgaccacac cttcacgtgc gtcctcgact
     6961 gccgctgggc gtacgggtcg cacggcacga tcgtcgacct gcgggacccg gctaacccgg
     7021 ccgtggccgg tgactggagc gagccctcgc tctcgtactg gcacgacgtc accgaggtcg
     7081 ctcccggccg tgtgctcacg gcgtcgatgc cgacgatgag gctgctggac gggcgcgagc
     7141 gcgtgacgaa accgcggcac gtcgccatgg gcgacgcgac gtcgagcggc acgaccggct
     7201 acctggggac cgtgctgtgg ccgcgtgagg ggaaggaccg gttcatcctc ggctccgagg
     7261 agacgtcgtt caatccgcag tgcagcgaca cgacgtccgc gttcgtgacc tgggacgcgt
     7321 cgcgttggcg caagacccgc acgttcgcgg tcgccgacca cttccggctg ggcaacggca
     7381 cgctcgccga cggctcaccc ccggcgagcg tcgtcggttg ctcgacgcac gtcttcgacg
     7441 agcacccggg gttccgagac ggaggcctgg tggcgctggc gcactacgag cacggcacgc
     7501 gcatcctcga gatcggccgg gacggctcga tagaggaggc ggcctggttc ctgccggcag
     7561 gcgggtcgac ctcggcgtcc tactggatga gcgacgagat cgtgtactcg atcgacttcc
     7621 acagggggct ggacatcctg cgctacacgg gggccgccta gggccggcgg cggcccgcgc
     7681 cggacgtgga aaggccccgc tcacacgagc ggggcctcga acctaccggc ggtctagaac
     7741 tgcgccgagt cgccgcaggc gcaggtgccc ttcgcgttcg ggttgttgat cgtgaagccc
     7801 gactgctgga gcccgtcgac gtagtcgacc tcggcccccg cgaggtacgg ggcgctcatc
     7861 ttgtcgacac ggatctcgac gccgtgcgcc gtgacgacct tgtcgtcgtc gagcttgcgg
     7921 tcgtcgaagt agagcgcgta gcggagcccc gcgcagccgc cgggctgtac ggcgatccgg
     7981 agcgcgtact ccttgccggc gtgctcctcc ggcgaggcgg agacctcacg ctcgagcagc
     8041 tcggtcacct tcgaggcagc cgagggggtg atgccgatct cgctctgggt cagttcctgg
     8101 gtggacaagg tggacctcct aggtttgccc agccgactgg ccgcgctggg gcgactcgat
     8161 tatatggaac ctcagtgcac gcccagccat tccacggcca gtagaagacc cgcaaacgcc
     8221 cccaaaccga gcagataggg ccgggcacgg gcgagtccct cgggctccgt ctcgaacacc
     8281 agcggcgcca gcaagacgag ggccaccgcg gcccccccga acagcgacgc cgcgaggacc
     8341 gtgacgtgcc agacctcgtg ccagctcgtc aatgggtggt gccggacgag aagtggaacc
     8401 tccccaccct gatcgccggg gcgacggtcg agccgaacga ggcgaagtcg ggggcgaacg
     8461 cgtgcaggtc ccgcccgacg ccgagcacgg acgacagcgt gtcgaggacg ctctgcgaga
     8521 agcggaggtt gcgcaccggc tcggcgatcc ggccgtcccg gatccgaaac gtcccgtcgc
     8581 gcgtcatccc cgtgagcaac gtcgagggcc ggtcgaggac gttcacgtag tggaacctcg
     8641 tcacgagcaa gccgtcttcc acggccccga cgaggtcctc gtagctctgg tcccccacct
     8701 cgaggacgac gttgctcgcg tacggccccc actcgttcga gccggagtag tggccggacg
     8761 gcgtcacctc catctgggcg gccgtccgca ggtcggtcac cggctcgcgt gcgaccccgt
     8821 cctcgatcac gtcgacgcgg cggcgcggca cgccttcgaa gtcgaacccg atgcctaccg
     8881 agcgcgggtg ccacgcgtcg tcggcgatcg tgaccgaggg ggccgccacc tcgtcgcccg
     8941 tcctggtcga caagaagctc tcgccgtcga tcacctgctt cgcgccgaac ccgacgtagg
     9001 agaggtagtc gagcatggtc gcgaccgcgg gggcctcgag cacgacctcg tagtcgcccg
     9061 gctcggcatc gccggcgtcg tggcccgcgg cggccttctc ggcggcaagc accccgacgg
     9121 cgggccagtc gaggtccccg atcgcgtgcg acgagcactc tccccagccc gtggcctcgc
     9181 cgtcgtcgac gagagccgtc gcgaccgcgc gcgtatacga gtcgaagcac gcgatgccgt
     9241 gggagttgaa gacaccgtag gcctgcacgc ccgtctcgac gacgcccgcc gcgttcccgc
     9301 ctcccgccgc cccgaggatc gccttcaccg cagccgcccg gtccgtcgcc gacgcagcga
     9361 ccgtcggcgc gtcgaccctc tcgatcgcct ccgccctgcc cacgacctcc gggtcggcga
     9421 ggcccgtgaa gccggagtcg ggcaacgacg accgagcagc ctcgagcgcg tgctgcgcag
     9481 ccccctcgag gcgctccggg ctgagctggt tcgtcgtcgc ggtcgcgagg cgcccttcgg
     9541 tgacgacccg cacgtagacg cgcagctcgt cccgctcggt cgcctggatg atctgcgagt
     9601 tcgcgtagcg cgtgacgccg ctcgccgacg cgtgcacggt gacctcgacg ccgtccgcgc
     9661 cgggcagccc gaggaccgct tccgccgcgg cccgcgcgtc gtcgaagccg aggcggaggc
     9721 cgctcattgc gaggtcccgg tcgagacgtt gcggaagcgc gcgggggccg tcccgtggcc
     9781 gacccgcatc gtctggccgg gctgaccctt gccgcagttc ggcgtgcccc agatcctcca
     9841 ctcgtccggc cccgcgatcg cgtcgcagcc cgcccagaag accggcgtga tgccggtgta
     9901 gcgcgggttg cggagcatcc ggccgagccg gccgttcttg atctcccacg cgatctcgca
     9961 cccgaactgg aagttcttgc gcttgtcgtc gatcgaccac gacttgttgg tcgccatgta
    10021 gatgccgtcg tcgacgtcgg cgagcaggtc ctcgaacgac ccggcccccg gcaacaggtt
    10081 gacgttcgtc atcctgatca gcgggaagtt ctcccacccc tccgcgcgca tcgtcccgtt
    10141 cgagcggtcc gcgccggcgg cggccgcggt ctcgcgcgac gtctggaacc cgaccagcac
    10201 tccgtcgcgc acgaggtcga cgcactgcgc ctcgacaccc tcgtcgtcgt agccgaacgt
    10261 gccgagccct cccggagtcg tcgcgtcgga cacgatgctg acgagctcgg acccgtaccg
    10321 cagcgagccc aggtccggga tcccgacgaa ggacgtcccg gcgaacgcgg cctccatccc
    10381 gaggacgcgg tcgagctcgg tcggatgccc gaccgactcg tggacctgca acatcacctg
    10441 gtggccgtcg agcacgaccg tggtgtcacc cgcgggacag tccggcgccg acagcagcgc
    10501 gaccgcctcc tcgccggtct gctgcgcgtg cgcctcgagg tccatcgccg tgacgatctc
    10561 gtagccgccg gcctcgaggt gcccccggaa cgagccggga tacgaccgcg tctgcacctc
    10621 cgacccctcg accgccgtgg cctcgatccc ggcccccgta tggatgatgg tctggtcgat
    10681 cgccgcgccc tccgaagaga cgaagaacgt gcgctgcttg agcacgtcca tcgtcccgcg
    10741 ggccgcacgg atcgcgggag acaccgacat ggcccgcgtc gccgacgcca gcagccctac
    10801 cttgtcctcg aggctcaccg agaacgggtc gacctccaac ggcgacgacc acgtagcgcg
    10861 atgcgggggc tcggggacga gctcgacggg gctctccagc gccgtcgccg tcgcccgtgc
    10921 gacctcgacc gcggcgcgag ccgtcgacgc ggcggagcct tgctcgaggt cgtacgaggc
    10981 cgcgaagccc cacgcgccgt cgaccaggac gcgcacgccg aagccttcgg agtcggaacg
    11041 ttccagcgcc tcgacgacgt ctcctcggac cgacaacgct tccgacgcgg actcgacgga
    11101 acggacgtcg gcatacgagg cccccgccgc caccgcggcg tcgagcgctg cttcgagcag
    11161 gtcctccatc acgcgaggtt cgacgaccgc gggtaggcca cggacgagtc cgtgatcacg
    11221 ttgacgacgg ccggctcgcc cgagtcgaac gccgccttca gcgcgggcgt caggtcgtcg
    11281 gggtcctcga cgtaaaaacc ccggcccccg aacgcctcca tcaccttgtc gtaacggatg
    11341 cccgggcgga gctccgcggc cacgtcgtac ccgtacagcg cctgcatcgg gtacttctcg
    11401 agggcccaga tgccgttgtt gccgacgacg cacacgaccg gcagcttcag gcgcacgagc
    11461 gtctcgagct ccatccccga gaacccgata gcgccgtcgc cgtacatgac gacgacctgg
    11521 cggtcgggat gcgcgagccg cgccgccatc gcatagccgg ggccgacgcc gaggcagccg
    11581 aacggcccgg tgtcgagcca gcaaccgcct tcgtacgacg ggatctcacg cccggcgaac
    11641 gaggcgaagt ccccgccgtc gcagatcacg accgcgtcgc gatcgagcag cggcacgagg
    11701 tcgccgtaca cacgctgggg gtggatcggg tgcgacgacg acctgaggtc ggcctgcgcg
    11761 gcgtcgcgct tcgcgtcctc ggtctcgcgc agcgcctcgg cccaagcctt cgtgtcgggc
    11821 ggcgacgaca gcgcggcggc gatcgcttcg aacgaggctt tcaggttcgc cccgacgccg
    11881 gccgcgaggt cgacgtgctg ggcgatcagg tcggcgtgcg agtccaggtg cacgaccttg
    11941 gcccccggcg cgaacccctg cccgaaggcg agacggaagt ccatcgacgt cccggccacg
    12001 accacgaggt cggcttcctt gaaagcgcgg ttccggctgc gcgagaagca gagccggtgg
    12061 tccgccggaa gacagccgcg gccctgcccg ttcgtgaacg tcgggagctc tgccgtctcg
    12121 acgaggtccc gcagcgcggt ctcggctccg tcgagccaca cgttcgaccc ggcgatcaat
    12181 acgggccgct cggcctcgct caggagcctg gcgacctcgt cgacgtcggg agcggcgggg
    12241 gccgggatct cgggagccgt caggtcgccg ccactccagt tgttgaacag cacctcgagc
    12301 gggaagtcca ggaactgcgg cccccgcggc ggcgtcagcg cggcgagcgc ggccgcgaac
    12361 acgtctccgg cgacgtcctc ggtcgaggcc accgtgcgcg cctttgcaag cggctcgacg
    12421 atggggacgt ggtcgatctc ctgtagcgac ccctgccccc agcggaagcc gggcgcgcgc
    12481 ccggcgaggc acaggaccgg cgcgccgttg aacccggcct gggcgagggg gctgagggcg
    12541 ttcgtgacgc ccggcccggc ggtgacggca cacacaccga gactccgagt gaggcgtgac
    12601 catccttcgg cggcgaacgc tgccgactgc tcgtggcggg tgtcgacgat gcggatgccg
    12661 tcctgatggg cgccgtcgag gatcgggaag atgtggccgc cggtgagcgt gaagaggtgc
    12721 tcgaccccga gcgccctcaa agcccgggta acggcgcgcc cgccgtggag ctgttcggtc
    12781 agtcgaggca gcctatctga gggccgcgcg agccccctcc gaatttgtca gacatttgtc
    12841 tgacaaatag cgagggccgc gcgatgcccc gcatcggcgc
//
LOCUS       DASQDO010000080         8652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1171526, whole genome shotgun sequence.
ACCESSION   DASQDO010000080 DASQDO010000000
VERSION     DASQDO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171526"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VEV43_02505"
     CDS             complement(<1..452)
                     /locus_tag="VEV43_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22420.1"
                     /translation="MLATRFRTRHVAALAAVVALVAAGLLQVVSTADAKPDGSAGKAV
                     AHYAGGKVIRSGAAAANAPEAALYYPGINALEPTLGLNKDGDVFFVGVPTAGAAIMRS
                     TDQGKTWEDKSPNLAGVKTHPVTLDPYVYVDEWTGRVFTIDLTVACSY"
     gene            573..1577
                     /locus_tag="VEV43_02510"
     CDS             573..1577
                     /locus_tag="VEV43_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HYP22421.1"
                     /translation="MEATIPPAVRPGATVALVAPSFGAIGAWPHRTERATAYLRSLGL
                     EVRAMPNAARNEGWASAPPEARAEDLHEAFSDDDVDVVLCAIGGNHCNQILPHLDFDL
                     IGSHPKVFQGYSDVSVLHWAFRRRSRLATFYGPALVPELGEFPAPLPYTDRWLRAAWF
                     RTAPLRYEPATEWTEEFLDWDLKADVRPRVTKPSPGWRTIRGGIAEGPVTGGCLETVC
                     WHVFGSADWSVPEGSIFLLETSEEAPSPAHVDGYLTDLEQAGVFDACTGLLFGRPMGY
                     RDEDVEVLWEVVEKRTAASGIPVLAGIDCGHTDPMMTIPFGVPARLDAGAEAFEVLLG
                     "
     gene            complement(1574..2692)
                     /locus_tag="VEV43_02515"
     CDS             complement(1574..2692)
                     /locus_tag="VEV43_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22422.1"
                     /translation="MNGNKKVRGVVAAVALVTAFAAAPSSATGFSSSNVEYVTTVPIE
                     AGAPTGARLVGDYLYVAGAKSFSIYDVSDPVNPALQSITPVGFQFANEDVDTNGEVLI
                     MSADTGVLKLFVWDVTDKAKPVKLAELANMRDHNFACVLDCTWAYGSRGTIVDLRDPA
                     SPRVAGSWGGGVTPGDGFDTTEVSPGIVLASTRILRLLDARKNPAQPKVLASATTHDN
                     RLLHSSRWPRGGKDRFALVQEETFAEAQCDEDSGTFMTWDTRRWRKTGTFRLVDSYRT
                     TGGTYADGSPAANALGCTAMWFQEHPRFRDGGLVTAAWFEHGTRFLDVASNGRITEVG
                     HFLPAGGSTIASYWITDEIVYSIDVVRGIDVLRFDSAS"
     gene            complement(2791..3690)
                     /locus_tag="VEV43_02520"
     CDS             complement(2791..3690)
                     /locus_tag="VEV43_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HYP22423.1"
                     /translation="MPLRRTLTIDGPLDLRLTLRPLRHGGGDPTVRLAPRAACRATRT
                     PAGSATLSIAVRPPEVEVEAWGPGASWCLDRVDGLLGLHDDPDAFRPADAVVRDLHRR
                     ARGLRIGRSGAVTESIVPAILEQKVTSEEAHRAYRMLVWAHGEPAPGPLELRLPPPPG
                     LLATLPYHAFHRFGVERRRADTIRRACSYAHRLEEAAGLPANEARRRLLALPGVGPWT
                     AAVVAGAAFGDPDAVIVGDLHLPRLVSWTLAGEPEGDDARMIELLEPYAGQRARVVRL
                     VVARGSRPPAHHPRRRMRSIAAI"
     gene            complement(3797..4462)
                     /locus_tag="VEV43_02525"
     CDS             complement(3797..4462)
                     /locus_tag="VEV43_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22424.1"
                     /translation="MKRLVAAALVCAVVGVVAATPAGAATYPRARTVLPTAGFALTGD
                     GTEYRNFDLQNNYVTTKTAGLPFEVRTDWQSGRRLFLNITSNASNVNYPAISYTCVTG
                     PEDPLRPTNKNSPALGSAFYLSTNRLVANIQCYNAAHDAGYFVSFINPQDCITVTQSP
                     STDPRFEGGDHYVIEGPNCTAHVSVRSGTKLTQIKSGSGKSAKPVVFNVPIYLEFDSE
                     PAI"
     gene            complement(4667..5674)
                     /locus_tag="VEV43_02530"
     CDS             complement(4667..5674)
                     /locus_tag="VEV43_02530"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HYP22425.1"
                     /translation="MRRRFSLCSAIALIASTVGVMTAAPVAASATVTYSGRGIAFSGS
                     VAGVATGFADTLPLPSSGGHQTASSISAGLPSLLGGDTLHASAFGQNDRTRTEAAASS
                     IAITTGSETITADFAMSRAMAVSSHGAPVLSGSSQVSNLVVNGVPIAVTGVANQLVSL
                     PSGDLVVNEQISSTSGATGSITVNALHLTLTDGTDLLVSSSRAGVTAGSSNCASFRQP
                     TTGGGWIIGQSTGKGTFGLSARPTANGFHGHVVYKDHGTGTRIHGPVTLYQEFGNGAL
                     LQGPATQNGQSVGNFIVEIQDNGEPGNSDTFSITTESMPPHAAGGPLQGGNIQMHKPC
                     K"
     gene            6069..6587
                     /locus_tag="VEV43_02535"
     CDS             6069..6587
                     /locus_tag="VEV43_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYP22426.1"
                     /translation="MRGKENGFTLSELLVVTLIIGIALTLGAPAFSRYWKVRSLNAAV
                     DEVATELRGDQQDATSQSHPWVWGAWFKQGTSRWGIVRGNANTGACEVRDRRSFPAGV
                     TVSAVSFADVTTPTLSAQCAAAAESGAEVVLFFARGSATGGSVSLSHPEVGGGSARTI
                     TVAPITGRVTKP"
     gene            6584..8563
                     /locus_tag="VEV43_02540"
     CDS             6584..8563
                     /locus_tag="VEV43_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYP22427.1"
                     /translation="MTTTNRREHGLTLIETLMAITIFSIMTLGIVPLLGTAMSGGAAT
                     RTESVARNVTAKTMERLRGLDYHVAYSSVAREVDLLDHFFPGRTPSFVPSVGTGFDAA
                     TSSYVTTCDSTSTSKACRALPDGSELPDGVLVEVRATFRTPENPSVTAPVPLAYSWNS
                     TGNDGPPSDLLEVEVTTVWSVGASARSFDLTTYLGARGESAAPSSGGGGGSPPPTSGP
                     PPPPTTVKLRSEARIDYGYEVTTTYQDTQSPPRMSDYTGTLGTAVAYGEQLESGSKAE
                     LSVSAGRLQIVRPANPAVSGDQGFHVDVSGATLDARAPADATTTTVTTAPAVSATTTE
                     VPATIGSLAASEAGTLTGARGAGPTVANGLPFVRGYYDINGTTAMSPMTSAPNHFWTT
                     PQTPNAGSGTETSINPLGLYPTSGATHKMITVSDWQFSSPSANVDPRGEVMVDSTPTT
                     PASSRTVTATATIPAHGQIVLFPMWYSSTNNGYMQIREFSASVSCAANADPGTASQAS
                     GSWSAHLSYNSYEGTRKQDFGVRLRQVTLPVQTRTDNPHVPYGDTNPLQTIRDLNNGN
                     GPKIYDADQNPGDTSLDAFMFAANGKRGLLTGWSQGTVQTSISADDRVVSAQLNGAIR
                     FETAPVYGPWGASQRPNSDTTFAMGKLSCKAEDYR"
BASE COUNT         1278 a         3038 c         3034 g         1302 t
ORIGIN      
        1 atgtacgagc aggcgacggt gaggtcgatc gtgaacacgc ggcccgtcca ctcgtcgacg
       61 tagacgtagg ggtcgagcgt cacggggtgg gtcttgacgc cggccaggtt cggggacttg
      121 tcctcccagg tcttgccctg gtcggtggat cgcatgatcg cagcgccggc ggtggggacc
      181 ccgacgaaga acacgtcgcc gtccttgttc aggccgagcg tcggctcgag cgcgttgatg
      241 cccgggtagt acagcgccgc ctcgggcgcg ttcgcggcgg cggcccccga acggatgacc
      301 ttgccgcccg cgtagtgggc gaccgccttg ccggcggagc cgtcgggctt ggcgtcggcg
      361 gtcgagacca cctggagcag cccggccgcg acgagcgcga ctacggcggc aagggctgcg
      421 acgtggcggg tgcggaaccg ggtggcgagc aagaagaccc cctggagacg gacatgacgg
      481 gaccagtcga ggtgctgggg cctagattcg ccgccgtagg cctgttcccc tccgtgagcg
      541 ggatgcccga ttcgtcctca taggctggcg cggtggaagc gacgatcccc cccgcggtcc
      601 ggcccggagc gacggtggcc ctggtggcgc cgtcgttcgg cgcgatcggg gcctggccgc
      661 accgcaccga gagggcgacc gcctacctcc gctcgctggg gctcgaggtc cgggcgatgc
      721 cgaacgccgc ccggaacgaa ggctgggcgt cggccccgcc ggaggcgagg gccgaggacc
      781 tccacgaggc gttctccgac gacgacgtcg acgtcgtcct gtgcgcgatc ggcggcaacc
      841 actgcaacca gatcctcccc cacctcgact tcgacctgat cgggtcgcac ccgaaggtgt
      901 tccagggtta ctccgacgtc tcggtgctcc actgggcgtt ccgacggcgc tccaggctcg
      961 ccacgttcta cggcccggcg ctggtgcccg agctcggcga gttccctgcg ccgctcccct
     1021 acaccgacag gtggctgcgg gcggcctggt tccgcaccgc gcccttgcgg tacgagccgg
     1081 cgaccgagtg gacggaggag ttcctcgact gggacctgaa ggccgacgtc cggccgaggg
     1141 tgacgaagcc ttcgccgggg tggcggacga tccgcggcgg catcgccgag ggccccgtca
     1201 ccggtgggtg cctagagacc gtctgctggc acgtgttcgg gtcggccgac tggtccgtcc
     1261 cggaggggtc gatcttcctc ctggagacgt ccgaggaggc cccctcgccg gcgcacgtcg
     1321 acggctacct caccgacctc gagcaggccg gcgtcttcga cgcctgcacg gggctgctgt
     1381 tcggacggcc gatgggctac cgcgacgagg acgtcgaggt cctgtgggag gtggtcgaga
     1441 agcggaccgc ggcgtcgggg atcccggtgc tggcgggcat cgactgcggc cacaccgacc
     1501 cgatgatgac gatcccgttc ggggtgccgg cgcgcctcga cgccggggcg gaggcgttcg
     1561 aggtgctgct gggctaggaa gcggagtcga agcgcaggac gtcgatccct cgcactacgt
     1621 cgatggagta gacgatctcg tccgttatcc agtagctcgc gatcgtcgag ccgccggccg
     1681 ggaggaagtg ccccacctcc gtgatgcggc cgttcgacgc gacgtcgagg aagcgcgtgc
     1741 cgtgctcgaa ccacgccgcg gtcaccaggc ccccgtccct gaagcgaggg tgctcctgga
     1801 accacatcgc cgtgcagccg agagcgttcg cggcggggct cccgtccgcg tacgtgcccc
     1861 cggtcgtgcg atacgagtcc acgagccgga acgtcccggt ctttctccac cggcgcgtgt
     1921 cccacgtcat gaacgtgccc gagtcctcgt cacactgcgc ctcggcgaac gtctcctcct
     1981 gtacgagggc gaaccggtcc ttgcccccgc gcggccaccg gctcgagtgg agcagccggt
     2041 tgtcgtgggt cgtcgcgctc gcgagcacct tcggctgcgc cgggttcttc cgcgcgtcga
     2101 gcagccgcag gatccgcgtc gacgcgagga cgatcccggg cgacacctcg gtcgtgtcga
     2161 agccgtcgcc gggggtgacg ccgccgcccc agctccctgc gacgcgcggg ctcgcggggt
     2221 cccggaggtc gacgatggtg ccgcgcgagc cgtaggccca ggtgcagtcg aggacgcagg
     2281 cgaagttgtg gtcgcgcatg ttcgcgagct cggcgagctt gacaggcttc gccttgtcgg
     2341 tgacgtccca cacgaacagc ttcaggacgc cggtgtcggc cgacatgatc aggacctcgc
     2401 cgttcgtgtc gacgtcctcg ttggcgaact ggaacccgac gggcgtgatg ctctgcagcg
     2461 cggggttgac cgggtcggac acgtcgtaga tcgagaagct cttggcgccc gcgacgtaga
     2521 ggtagtcgcc tacgaggcgg gcccccgtgg gcgcgccggc ctcgatcggg accgtcgtga
     2581 cgtactcgac gttcgagctc gagaacccgg tcgcggacga cggagccgcc gcgaacgcag
     2641 tgaccagcgc taccgcggcg acgaccccgc ggaccttctt gttgccgttc atgcctcgcc
     2701 ctttcgccgt cccgggcacg gctcccgccc ccgggaccaa gttggccacg ctccagagat
     2761 gagcacgtag ccgcgccggg cgcgtaacgc ctagatcgcg gcgatcgaac gcatgcgtcg
     2821 gcgggggtga tgcgcgggcg ggcggctccc ccgcgcgacg accagccgga cgacccgcgc
     2881 ccgctggccc gcgtacggct cgaggagctc gatcatccgc gcgtcgtcac cctcgggctc
     2941 gcccgcgagc gtccacgaca cgagccgcgg gaggtggagg tcgccgacga tgacggcgtc
     3001 cggatcgccg aacgcggccc ccgcgacgac cgcggccgtc cacgggccga cgccgggcag
     3061 cgccaggaga cgccgccgcg cctcgttcgc gggcagcccc gcggcctctt cgagccggtg
     3121 cgcgtacgag cacgcccttc ggatcgtgtc ggcccgccgg cgttcgacgc cgaagcggtg
     3181 gaacgcgtgg tacggcagcg tggccagaag cccgggtggc ggaggcaggc gcagctcgag
     3241 ggggccgggt gcgggctcgc cgtgcgccca cacgagcatc cggtaggcgc ggtgggcctc
     3301 ctcgctcgtc accttctgct cgaggatcgc cgggacgatg ctctcggtca cggcccccga
     3361 ccgaccgatg cgcaggccgc gcgcccggcg gtgcagatcg cgcacgaccg cgtccgccgg
     3421 ccggaacgca tcggggtcgt cgtgcagccc caggaggccg tccacacggt cgagacacca
     3481 cgacgcgccc ggcccccacg cctcgacctc gacctcgggc gggcgcaccg ctatcgacag
     3541 tgtggccgaa cccgcgggcg tccgcgtcgc gcggcatgcg gcccgaggcg cgaggcggac
     3601 cgtcgggtcg cctccgccgt ggcgcagcgg gcggagggtc agccgcaggt cgagcggccc
     3661 gtcgatcgtc aaggtccggc ggaggggcat ggccccaccc taggcccggc gtgtgacgct
     3721 ttcgcggggg aagccgcggc gcgcgaaaaa aaagaggccc gggcttcgac ccgggcctcc
     3781 tgctaactat gggctgctat atggcgggtt cgctgtcgaa ctccaggtag atcgggacgt
     3841 tgaagacgac cggcttggcg ctcttcccgc tgccggactt gatctgcgtc agcttcgtcc
     3901 cgctccggac cgagacgtgc gccgtgcagt tcgggccctc gatcacgtag tggtcgccac
     3961 cttcgaaacg cgggtccgtc gacgggctct gcgtcaccgt gatgcagtcc tgcgggttga
     4021 tgaacgagac gaagtagccg gcgtcgtggg cggcgttgta gcactggatg ttcgcgacca
     4081 gcctgttcgt cgacaggtag aacgcggagc cgagagccgg gctgttcttg ttcgtcggcc
     4141 tcagcgggtc ctcggggccc gtcacgcagg tgtacgagat cgccgggtag ttcacgttgc
     4201 tcgcgttgct cgtgatgttc aggaagaggc gccgtcccga ctgccagtcg gtccgcacct
     4261 cgaagggcag tcccgccgtc ttcgtggtga cgtagttgtt ctggaggtcg aagttcctgt
     4321 actcggtccc gtcaccggtc agcgcgaatc cggccgtcgg caagaccgtt cgcgcccgcg
     4381 ggtaggtcgc tgcgcccgca ggcgtcgccg caaccacccc gaccaccgcg cacaccagcg
     4441 cagccgcaac cagtcgcttc atgtggacct cccagtcctc gtagcaccct tgcttacgtc
     4501 gtagcacctt gcttacctcg tgtcgggaac cgtatttacc gggtggtttg tcggcaatag
     4561 tccgattgga tcatttctcc aggtcacaat cttgccccgt ccggtgccgg acgcgcaacg
     4621 ggggccgcat ggagcgaccc ccgttccgta ctccgctacc gaaaactcac ttgcacggct
     4681 tgtgcatctg gatgttgccg ccctggagcg gccctccggc tgcatgcggc ggcatgctct
     4741 cggtcgtgat cgagaacgtg tccgagttgc ccggctcgcc gttgtcctgg atctcgacga
     4801 tgaagttgcc gacggactgg ccgttctgcg tcgccggacc ctgcaggagc gcaccgttcc
     4861 cgaactcctg gtacagcgtg accgggccgt ggatcctcgt tccggtgccg tggtccttgt
     4921 agacgacgtg gccgtggaag ccgttggccg tgggccgggc cgagagtccg aaggtcccct
     4981 tgcccgtgct ctggccgatg atccaacctc cgcccgtggt cggctggcgg aaggaggcgc
     5041 agttcgagct gccggcggtg acccccgccc gggagctcga gacgagcagg tcggtgccgt
     5101 cggtcagggt caggtgcagg gcgttgacgg ttatcgaccc cgtcgccccg ctcgtggagc
     5161 tgatctgctc gttgacgacg agatcgccgc tcggcagcga cacgagctgg ttcgcgacac
     5221 ccgtcacggc gatcggcacg ccgttcacga cgaggttcga gacctgcgag ctcccgctca
     5281 ggacgggcgc gccgtgggag ctgaccgcca tcgcccggga catcgcgaaa tcggccgtga
     5341 tcgtctcgga gcccgtcgtg atcgcgatgg aagaggccgc cgcctcggtg cgggtgcggt
     5401 cgttctggcc gaacgcgctg gcgtgaagcg tgtcgcctcc gagaagcgac ggcaaccccg
     5461 ccgaaatcga cgaggccgtc tggtgaccgc cgctggacgg cagcggcaag gtgtcggcga
     5521 agccggtggc cacgccggcg accgacccgc tgaaggcgat cccccgtccg ctgtaggtga
     5581 ccgtcgccga cgcggcgacg ggggccgcgg tcattactcc tacggtcgac gcgatcagcg
     5641 caatggcact acacaacgag aaccttcgtc gcacacagac ctcctcggat cgggtacgcg
     5701 ccgtgagcgg cgccggggac ttggtgcatc gtcgctccct cgaacgaggg cgccaatagc
     5761 tcacgcgggt ggtcttcggc atagcccact tccgacgcgg tgcctacggt ccggtagctc
     5821 cggcaacgca ttgttaaaac gccattaacc ggcctgccgg gggtttcggg acccgccctg
     5881 caacaagcgt ctcgactaag tgactagttc gtatagtccg cggcgggcag gggccgggac
     5941 accccccggc aagaacgcgc tccgtcgccg gcgcctcctc cgcccccgcc aggattcaag
     6001 tgccgtcccg ccgccgccga taccggacac gaggcccgac tcgggcattc cttcctggga
     6061 gagggcggat gaggggcaag gaaaacgggt tcactctgtc ggagctattg gtcgtgacgt
     6121 tgatcatcgg gatcgcgctc acgctggggg cgcccgcgtt cagccgctac tggaaggtgc
     6181 gatccctcaa cgcggcggtc gacgaggtcg ccaccgagct gcgcggcgac caacaggacg
     6241 cgacgagcca gtcgcacccc tgggtgtggg gtgcgtggtt caagcaggga acgtcccggt
     6301 gggggatcgt gcgcgggaac gcgaacaccg gagcgtgcga ggtgcgcgac cggcggagct
     6361 tcccggcggg ggtcaccgtc agcgcggtct ccttcgccga cgtcacgacg cccacgttgt
     6421 cggcccagtg cgcggcggcg gcggagtccg gagccgaggt ggtcctcttc ttcgcccgcg
     6481 gaagcgccac cggcgggtcc gtgagcctgt cgcaccccga ggtcggcggc ggatccgctc
     6541 gcacgatcac ggtggcgccc atcacggggc gggtgacgaa gccgtgacga cgacgaaccg
     6601 gcgcgagcac ggcctcacgc tcatcgagac cctcatggcg atcacgatct tctcgatcat
     6661 gacgctgggg atcgtcccgt tgctcggcac cgccatgagc ggcggcgcgg cgacccggac
     6721 cgagtcggtg gcgcgcaacg tcacggcgaa gacgatggag cgcctgcgcg gcctcgacta
     6781 ccacgtcgcc tactcctcgg tggcgcgcga ggtcgacctg ctcgaccact tcttcccggg
     6841 ccgcacgccc tcgttcgtgc ccagcgtcgg gacgggcttc gacgccgcga ccagctccta
     6901 cgtcacgacg tgtgactcca cgtcgacgag caaggcgtgc cgggccctcc cggacggctc
     6961 cgagcttccc gacggcgtcc tggtcgaggt cagggctacg ttccggacgc ccgagaaccc
     7021 ctccgtgacc gcccccgtgc cgctcgcgta ctcgtggaac tccaccggga acgacggccc
     7081 cccgagcgac ctgctcgagg tcgaggtcac gacggtctgg tccgtcggag cctcggcgcg
     7141 cagcttcgac ctgaccacct acctgggcgc ccgcggggag tccgcggctc ctagcagtgg
     7201 aggcggagga ggctctcctc ccccgacgtc gggcccgccg cctcctccca cgaccgtgaa
     7261 gctccggtcg gaggcgcgca tcgactacgg ctacgaggtc acgacgactt accaggacac
     7321 ccagagcccg cctcgcatga gcgactacac gggaacgctc ggcacggccg tcgcgtacgg
     7381 cgagcagctg gagtccgggt cgaaggcgga gctgtcggtc agcgcggggc gactccagat
     7441 cgtgcggccg gccaacccgg ccgtctcggg cgaccagggt ttccacgtcg acgtgagcgg
     7501 agcgacgctg gacgcgcgcg ctcctgcgga cgcgacgacc acgaccgtga cgacggctcc
     7561 ggccgtctcg gcgacgacga ccgaggtccc cgccaccatc gggtcgctcg cggcctcgga
     7621 ggccggcacg ctcaccggcg cgcgcggcgc gggaccgacc gtcgcgaacg gcctcccgtt
     7681 cgtcaggggc tactacgaca tcaacgggac gaccgcgatg tccccgatga ccagcgcgcc
     7741 caaccacttc tggacgaccc cgcagacgcc caacgcgggg tctgggacgg agacgagcat
     7801 caaccctctg ggcctctacc cgacgtcggg cgcgactcac aagatgatca cggtgagcga
     7861 ctggcagttc agcagccctt ccgccaacgt cgacccgcgc ggcgaggtga tggtcgattc
     7921 cacgccgacg acccccgctt cgagcaggac cgtgaccgcg accgcgacga tccccgctca
     7981 cggccagatc gtgctcttcc ccatgtggta ttcgagcacc aacaacggct acatgcagat
     8041 ccgggagttc agcgccagcg tctcgtgcgc ggccaatgcg gatccgggca ccgcctcgca
     8101 ggcgtcgggg tcttggtcgg cacacctctc gtacaactcg tacgagggga ctcgtaaaca
     8161 ggacttcggc gtcaggctcc gccaggtgac tcttcccgtc cagacccgca cggacaaccc
     8221 tcacgtcccc tacggcgaca cgaaccccct tcagaccatc agggacctca acaacggcaa
     8281 cgggccgaag atctacgacg ccgaccagaa ccccggcgac acctccctgg atgcgttcat
     8341 gttcgccgcc aacgggaaga gggggctgct gaccggctgg tcccagggca ccgtgcagac
     8401 gagcatctcc gccgacgacc gggtggtgtc ggcccagctg aacggcgcca tcaggttcga
     8461 gacggccccc gtctacgggc cgtggggcgc gagccagagg ccgaactccg acacgacgtt
     8521 cgccatgggc aagctgagct gcaaggcgga ggactaccgg tgaccgctcg cgccgaagaa
     8581 ggcttcaccc tcgtggaggt cctggtcgcc atggcgctgg tctcgctgat cgcggccggc
     8641 tgcttgcagg tc
//
LOCUS       DASQDO010000081         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_314987, whole genome shotgun sequence.
ACCESSION   DASQDO010000081 DASQDO010000000
VERSION     DASQDO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314987"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VEV43_02545"
     CDS             complement(<1..583)
                     /locus_tag="VEV43_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYP22428.1"
                     /translation="MRAGTEARPRRTQADRRAQTRGALLEAAARGLSRHGYGNLVLER
                     VAAEAGYSRGALYHLFAGKEDLALAVVEWVERTWYEEMGPLIEGVDDPVEALIGVVRG
                     HAVYCRRDVARVMMTLRVEFGDQDHPVGRAVDAAVRRVVRDCTKLLAAARKQGAIPPG
                     PSPRLLGVAVVGAVEGLVIGLAGKAPYDELLAER"
     gene            621..1016
                     /locus_tag="VEV43_02550"
     CDS             621..1016
                     /locus_tag="VEV43_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22429.1"
                     /translation="MDVTLGTAGFLCLALAAGHTAIGVVWVLPGLDVERMPKTPFGPR
                     SLTLAMVRVTWFVVTILVVGLAGVLLTLAVAPDADAETVLLRWFAGAWVATTAMSLWA
                     GGGRGRQVWRFPVPLVWLVVAGLCWAASA"
     gene            1013..1591
                     /locus_tag="VEV43_02555"
     CDS             1013..1591
                     /locus_tag="VEV43_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HYP22430.1"
                     /translation="MRLPRSEHTSRPWRIHDVAHDFRLEDVWALPTPGGPGDFPRLVE
                     MVAALDPAQTSSPVVRMLFTIRLRMGALLGWDDESSGVGERVPSLRERLPDDLRDTVS
                     PAPFEAAPFEPLYRTDDEFAAEVANRTVHGVLHVGWVRADDGGYRGQLAILVKPNGLL
                     GSAYMAGITPFRHFLVYPTMMREFGRAWEAAR"
     gene            complement(1588..2322)
                     /locus_tag="VEV43_02560"
     CDS             complement(1588..2322)
                     /locus_tag="VEV43_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYP22431.1"
                     /translation="MDATQMLVGCCGLAGRQAVYYAEFPVVEVQRTFYQLPRLALAEK
                     WKREAPADFRFTMKAWQLVTHRADSPTYRRLREELPGASSAYGDFQDTEEVWLAWERT
                     LEWARSLQTPVVVFQCPRSFRPTDESVRNLRTFFERVERDEMAFAWEPRGWPDELVGD
                     LCRELDLVHCVDPLIDDTQWGPFAYHRLHGLPAYRYRYRYTDDDLDRIHRAALAELDA
                     GRSHVYVMFNNWWLHDDARRFVARFR"
     gene            2372..>3754
                     /locus_tag="VEV43_02565"
     CDS             2372..>3754
                     /locus_tag="VEV43_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYP22432.1"
                     /translation="METPSQTLHRLTCYEHGRDWDEPADDPRILRDLVPNDLDRLPWF
                     YKRYSGSLPVVPLPRDLPATAAPAVAVLAGTAEIARAELDLPHLSRLLHLSAGVVRTS
                     PRPYGTYLFRAAGSAGGRFPSELYVAVPEGGTLPPGVHWYHPQEHALVQVGRPPRGDA
                     VTVVVTGIPWRTGWRYAERGYRHVFWDAGTMLAQLLAAAESAGAPPALYSTFPDAAVA
                     ALVGADRVHELPVAVVALGEGEPALEATGPAAAGEVDAAPVEFPLVTPAYRAGERDAL
                     GAPWPAGEPVDVPGRGAAPVEDVILARGSQRLMDPARGLSQEVLLTSMSAALRGIDLP
                     HFVVVHDVEGLEPGVYRWPDLTAPVRKGDLREELYRVCLDQGLCSDAAFVVISAARVA
                     ELDDRAYRAAQLASGVVEGRLHLLAYALGASASGMTFDDSLIHALLGEPLDGLLFTCV
                     GVPEYAAKAGG"
BASE COUNT          462 a         1322 c         1401 g          569 t
ORIGIN      
        1 tcctctcggc aagcagctcg tcgtacggcg cctttcctgc gagaccgatc acgaggccct
       61 cgaccgcgcc gaccacggcg acgccgagca ggcggggcga ggggcccggc gggatcgcgc
      121 cctgcttgcg cgcggcggcg agcagcttcg tgcagtcgcg cacgacccgg cgcaccgcgg
      181 cgtcgaccgc gcgcccgact ggatggtcct gatcgccgaa ctcgaccctg agcgtcatca
      241 tcacgcgggc gacgtcgcgg cggcagtaga cggcgtgccc gcgcaccacg ccgatcaggg
      301 cctcgaccgg gtcgtcgacg ccttcgatca gcgggcccat ctcctcgtac caggtccgtt
      361 cgacccactc gacgaccgcg agcgcgaggt cttccttgcc ggcgaacagg tgatagagcg
      421 cgccgcggga gtagccggct tcggccgcta cgcgttcgag cacgaggttc ccgtagccgt
      481 ggcgggagag cccgcgcgct gcggcttcga gcagagcccc gcgcgtctgc gcccggcggt
      541 cggcctgcgt gcggcggggc cgcgcttcgg tcccggccct cactttgcct cacatgcata
      601 catgtgtgta tgttagctga gtggacgtca cactggggac cgcgggcttc ctgtgcctcg
      661 cgctggccgc ggggcacacg gcgatcggcg tcgtgtgggt gctccctgga ctcgacgtcg
      721 aacgcatgcc gaagacgccg ttcggccccc gctccctgac gctcgcgatg gtccgcgtga
      781 cgtggttcgt cgtgacgatc ctcgtcgtcg ggctggccgg cgtcctgttg accctcgcgg
      841 tcgctcccga tgccgacgcc gagaccgtcc tgttgcgctg gttcgccggc gcatgggtcg
      901 ccacgacggc gatgtccctc tgggccgggg gaggccgcgg ccggcaggta tggcgtttcc
      961 ccgtcccgtt ggtgtggctc gtcgtcgcgg gtctgtgctg ggccgcgtcg gcatgaggct
     1021 cccgcgctcc gagcacacgt cgaggccctg gcgcatccac gacgtcgcgc acgacttccg
     1081 gctcgaggac gtgtgggcat tgccgacgcc gggaggcccc ggcgacttcc cgcggctcgt
     1141 cgagatggtt gccgcgctcg accccgcgca gacgtcctcg cccgtagtgc ggatgctgtt
     1201 cacgatccgg ctgaggatgg gcgcactgct cggctgggac gacgagtcgt cgggggtggg
     1261 cgagcgggtg ccgtcgctgc gcgagcggct accggacgac ctgcgcgaca ccgtgtcgcc
     1321 ggccccgttc gaagcggccc cgttcgagcc gctctaccgg accgacgacg agttcgcggc
     1381 agaggtcgcg aaccggacgg tgcacggggt cctccacgtc gggtgggtcc gggcggacga
     1441 cggtgggtac cggggccagc tcgcgatcct cgtgaagccg aacggcttgc tggggagcgc
     1501 gtacatggcc gggatcacgc cgttccgtca tttcctcgtg tacccgacga tgatgcgcga
     1561 gttcgggcgg gcgtgggagg ccgcgcgcta gcggaagcgc gcgacgaacc ggcgcgcgtc
     1621 gtcgtgcagc caccagttgt tgaacatcac gtagacgtgt gaccggcccg cgtcgagctc
     1681 ggcgagcgcg gcccggtgga tgcggtcgag gtcgtcgtcc gtgtagcggt agcgatagcg
     1741 gtacgcgggg aggccgtgca ggcggtggta cgcgaacggc ccccactgcg tgtcgtcgat
     1801 cagcgggtcg acacaatgga cgaggtcgag ctcgcggcac aagtcgccga cgagctcgtc
     1861 gggccaaccg cgcggctccc acgcaaaggc catctcgtcg cgctcgaccc tctcgaagaa
     1921 cgtccggagg ttgcggaccg actcgtccgt cggacggaag ctgcgcggac actggaagac
     1981 gacgaccggc gtctgcaggg accgcgccca ctcgagcgtc cgctcccacg cgagccacac
     2041 ctcttcggtg tcctggaagt cgccgtacgc gctgctcgcg cccgggagct cttcgcgcag
     2101 gcggcggtac gtcgggctgt cggcccggtg cgtgacgagc tgccacgcct tcatcgtgaa
     2161 ccggaagtcc gcgggggcct cgcgcttcca cttctccgcg agcgcgagcc gcgggagctg
     2221 atagaaggtc cgctgcactt cgacgaccgg gaattccgcg tagtagacgg cctggcgccc
     2281 cgccaatccg cagcagccga cgagcatctg cgtcgcgtcc acgacggcag cgtacgcggg
     2341 ccccccggcg cgggacgcga gactgtcgcc cgtggagacg ccgagccaga cccttcatcg
     2401 ccttacctgc tacgagcacg gccgcgactg ggacgagccc gccgacgacc cgcgcatcct
     2461 gcgcgacctc gtcccgaacg acctcgaccg gctgccgtgg ttctacaagc gctactcggg
     2521 ttcgctgccg gtcgtgccgc tgccgcggga tctgccggcg accgccgcgc ccgccgtcgc
     2581 ggtgctggcg gggaccgccg agatagcgcg cgccgagctc gaccttcccc acctgtcgcg
     2641 gctgctgcac ctgtcggccg gagtggtgag gacgtcgccg cgcccgtacg ggacctatct
     2701 gttccgggca gcggggtcgg ccggagggcg gttcccgtcc gagctgtacg tcgccgttcc
     2761 ggagggcggc acgctgccgc cgggcgtgca ctggtaccac ccgcaggagc acgcgctcgt
     2821 gcaggtcggc cggccgccgc gcggtgacgc ggtgaccgtc gtcgtcacgg gcatcccctg
     2881 gcggacgggg tggcggtacg ccgagcgcgg ttaccgccac gtcttctggg acgccgggac
     2941 gatgctggcg cagctgctcg ctgcggcgga gtcggcgggc gctccgccag cgctctactc
     3001 gacgttcccg gatgccgcgg tggccgcgct cgtgggggcc gaccgggtgc acgagctccc
     3061 cgtagcggtc gtcgccttgg gcgaagggga gcccgcgctg gaggcgacgg gtcccgccgc
     3121 cgccggcgag gtcgacgcgg cccccgtcga gttcccgctg gtgacgcccg cgtacagggc
     3181 gggggagcgc gacgcgctcg gagcgccgtg gcccgcgggt gagcccgtcg acgtcccggg
     3241 ccggggggcc gcgcccgtag aggacgtgat cctggcgcgc ggttcgcagc gtctgatgga
     3301 cccggcccgg ggcctgtcgc aggaggtact gctcacttcc atgagcgcgg cgctgcgggg
     3361 gatcgacctg ccgcacttcg tggtggtgca cgacgtcgag ggcctcgagc caggcgtgta
     3421 ccggtggccg gatctcacgg cgccggttcg taagggggac ctccgggagg agctgtaccg
     3481 cgtgtgcctc gaccaggggc tctgctccga cgcggctttc gtcgtgatct cggccgcgcg
     3541 cgtagcggag ctcgacgacc gggcctatcg cgccgcgcag ctcgcgtcgg gtgtggtgga
     3601 gggccgcctc cacctgctcg cgtacgcgct gggcgcgagc gcgtccggga tgacgttcga
     3661 cgacagcctc atccatgcgt tgttgggaga gccgctcgac ggcctgctgt tcacctgcgt
     3721 cggcgtgccc gagtacgcgg cgaaggccgg gggg
//
LOCUS       DASQDO010000082         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_988502, whole genome shotgun sequence.
ACCESSION   DASQDO010000082 DASQDO010000000
VERSION     DASQDO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988502"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /gene="paaA"
                     /locus_tag="VEV43_02570"
     CDS             complement(<1..820)
                     /gene="paaA"
                     /locus_tag="VEV43_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HYP22433.1"
                     /translation="MTYEEKLARFEAHIEAGGKVEANDWMPDDFRRGVLKFIEMHANS
                     EIMGALPERECLPIAPTLQRKMSLCAKIQDEVGHAQLLYRVAEDLGKSRVAMYEDLVH
                     GKAKFHNVFHYPVKHWGDVAIIGWLIDGAALVTQAALLDSSYAPYTRVLKRICAEEQL
                     HLRHGEDITLELASGTDEQREMFQEALNRWWLAIIHFFGPRSNVAKDLLLKWKVKTRT
                     NEDLRNEFLDRYVPKIYELGFTIPAPVPVQTEDGHWELVEDEFDWAPLDAIKRNE"
     gene            complement(817..1401)
                     /locus_tag="VEV43_02575"
     CDS             complement(817..1401)
                     /locus_tag="VEV43_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYP22434.1"
                     /translation="MAEAALRTRRADVVKAAARLFSERGYHGTSMQHLGDALGLQRGS
                     LYAHIGSKEELLFDVVEEGAERFLERGQRALDGDGSATERLTALLVGHAETAAEHLDS
                     ATVFLNEWRYLSEDLRAAVQAKRDRYEAMVRSIVDEGIATGEFRADADVRFAATLVLS
                     AGNWLFSWYRPGGELGPTEIGERFAELLTRGLSR"
     gene            1465..2169
                     /gene="nth"
                     /locus_tag="VEV43_02580"
     CDS             1465..2169
                     /gene="nth"
                     /locus_tag="VEV43_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYP22435.1"
                     /translation="MPVPDRDAPAPERLPHVLRRLKKAYPEAKIALYYTTPLECVVAT
                     VLSAQSTDAKVNEVTADLFRKYRKPEDYLAVPEEELQRDIHPTGFFRQKTKALRGLSQ
                     KLLDDFDGEVPRSIKELITLPGVARKTANIVLVNCYPEAARKDPDAGIAVDTHVGRLA
                     VRLAFTGYGSKEAEKIERDLMELAPRKQWPQVPYLLIEHGRAICLAKKPLCAECPVEK
                     LCPSSQVAGRPDLYRR"
     gene            2166..3140
                     /locus_tag="VEV43_02585"
     CDS             2166..3140
                     /locus_tag="VEV43_02585"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006866027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYP22436.1"
                     /translation="MTTYGGSVKKVILAAPRGYCAGVDRAVQMVERALEKLGAPVYVR
                     KEIVHNRHVVEELANRGAIFVDSEEEIPEGAVCIFSAHGVSPEVRRKAQERNLQVIDA
                     TCPLVTKVHLEAKRFARDGRRIVLVGHEGHEEIEGTAGEAPDATTVIERPEQIDELGL
                     DPDEPVSYLTQTTLSVDDTLNVVEALRERFNDIRGPRSDDICYATQNRQVAVKAIAPE
                     ADVVLIVGAINSSNSNRLVEVARVAGTPGYLVPDETYLDPAWLEGAETVGVSSGASAP
                     EVLVERLLAKLADLGYRQVETREVTTEDVTFSLPPWLREERSAVTGSG"
     gene            3438..>4558
                     /locus_tag="VEV43_02590"
     CDS             3438..>4558
                     /locus_tag="VEV43_02590"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP22437.1"
                     /translation="MSVGQRLRRLRLQSGLTQKELADPRYTHAYVSTIEAGRRMPSAT
                     ALEHFASKLSVDVEELRTGRPPDLEPKLRLKLQGARRDISAGDLEEAAKALTQVLRDS
                     KRYKMSHLEAKAVEYQALVEERRGNMQAAFEIYQDVEKILIEAPPPMLADCMAGQARC
                     LIGLGDMRYAIHLVESLVERMEREGFRDPNAMVRLYAPLVWAYAQLGVQSKASEYAGR
                     ALELLPKVTDPFNLAVMQVNLAEAYLNEGRIKEADAALLRAEDLFENLELRSESAAVH
                     MNRGYSLVRGGGDLKIARTELEKALELYVSMGDERGEAHARNELAHVARVEGDVTEAR
                     KQLDRSLKLLKNSEDVGELALAYLELGLTIKEDDPKKGE"
BASE COUNT          795 a         1545 c         1510 g          708 t
ORIGIN      
        1 cctcgttgcg cttgatcgcg tcgagcggag cccagtcgaa ctcgtcctcg acgagctccc
       61 agtggccgtc ttccgtctgc acggggaccg gagccgggat agtgaagccg agctcgtaga
      121 tcttcggcac gtagcggtcg aggaactcgt tgcgcaggtc ctcgttcgtg cgcgtcttga
      181 ccttccactt gagcaggagg tccttggcga cgttcgatcg ggggccgaag aagtggatga
      241 tcgcgagcca ccagcggttg agcgcctcct ggaacatctc gcgctgctcg tcggtgcccg
      301 acgcgagctc gagcgtgatg tcctcgccgt ggcggaggtg cagctgctcc tcggcgcaga
      361 tgcgcttgag cacccgcgtg tacggggcgt acgaagagtc gagcagagcg gcctgcgtga
      421 cgagcgcggc cccgtcgatg agccagccga tgatcgccac gtcgccccag tgcttgacgg
      481 ggtagtggaa gacgttgtgg aacttggcct tgccgtggac gaggtcctcg tacatcgcga
      541 cgcggctctt gccgaggtcc tcggcgaccc ggtagagcag ctgcgcgtgg ccgacctcgt
      601 cctggatctt ggcgcagagc gacatcttgc gctggagggt cggggcgatc ggcaggcact
      661 cgcgctcggg gagcgcgccc atgatctcgg agttcgcgtg catctcgatg aacttgagga
      721 cgccgcgccg gaagtcgtcg ggcatccagt cgttcgcctc gaccttcccg ccggcctcga
      781 tgtgcgcttc gaaccgcgcg agcttctcct cgtacgtcat cgtgacaacc cccttgtcag
      841 tagctccgca aacctctcgc cgatctcggt gggccccagc tctcctcccg ggcggtacca
      901 ggagaacagc cagttcccgg cggagagcac gagcgtcgcc gcgaaccgta cgtccgcgtc
      961 ggcccggaac tctcccgttg cgatcccttc gtcgactatc gagcggacca tcgcctcgta
     1021 gcggtcacgc ttcgcctgca ccgcggccct gaggtcctcg gacagatagc gccactcgtt
     1081 caaaaagaca gtggcggagt cgagatgctc cgcggcggtc tcggcgtggc cgacgagcaa
     1141 cgccgtgagc ctttccgtcg cggacccgtc gccgtccagc gctcgctgcc cccgctcgag
     1201 gaaccgctcg gccccctcct cgacgacgtc gaacagcagc tcctccttcg agccgatgtg
     1261 cgcgtagagc gagccgcgct ggagccccaa agcgtcgccg aggtgctgca tcgaggtgcc
     1321 gtggtagccg cgctcggaga acagccgggc cgcggccttc accacgtcgg cacgtctggt
     1381 cctgagagct gcctctgcca tccggtccct tccggttcca aacaaacggt cgtttggaaa
     1441 gcctagcaga tacccttgcg tccgatgccc gtgcccgatc gcgacgcccc ggcccccgag
     1501 cgcctcccgc acgtcctgag gaggctcaag aaggcctacc ccgaggcgaa gatcgccctg
     1561 tactacacga cgcctctcga atgcgtcgtc gcgaccgtgc tctcggccca gtcgaccgac
     1621 gcgaaggtca acgaggtcac ggcggacctc ttccgcaagt accgcaagcc cgaggactac
     1681 ctcgcggtgc ccgaggagga gctccagcgc gacatccacc cgacagggtt cttccggcag
     1741 aagacgaagg cgctccgggg actgtcgcag aagctcctcg acgacttcga cggcgaggtt
     1801 ccccgctcga tcaaggagct gatcacgctc cccggcgtcg cccgcaagac ggcgaacatc
     1861 gtgctcgtca actgctaccc ggaggcggcg cgcaaggacc cggacgccgg gatcgcggtc
     1921 gacacccacg tcggccggct cgccgtccgc ctcgccttca cgggctacgg ctcgaaggag
     1981 gccgagaaga tcgagcgcga cctcatggag ctcgcccccc gcaagcaatg gccgcaggtc
     2041 ccgtacctgc tgatcgagca cggccgcgcg atctgcctgg cgaagaagcc gctgtgtgcg
     2101 gagtgtcccg tggagaagtt gtgtccttcc agtcaggttg cgggaaggcc ggacctctac
     2161 agacgttgac cacctatggt ggaagcgtga aaaaggtgat cctcgcggcc ccccgcggct
     2221 actgcgccgg cgtcgaccgc gccgtccaga tggtcgagcg cgcgctcgag aagctcgggg
     2281 cccccgtcta cgtccgcaag gagatcgtcc acaaccgcca cgtcgtcgag gagctcgcca
     2341 accgcggcgc gatcttcgtc gactcggagg aggagatccc cgagggcgcg gtgtgcatct
     2401 tctccgccca cggcgtgtcg ccggaggtcc gccgcaaggc gcaggagcgc aacctccagg
     2461 tcatcgacgc cacctgtccg ctcgtgacga aggtccacct cgaggccaag cgcttcgcca
     2521 gggacggccg ccgcatcgtg ctcgtcgggc acgagggaca cgaggagatc gagggcacgg
     2581 cgggcgaggc ccccgacgcg acgacggtca tcgagaggcc cgagcagatc gacgagctgg
     2641 gcctcgatcc cgacgaaccg gtgtcgtacc tgacgcagac gacgctgtcc gtagacgaca
     2701 cgttgaacgt cgtcgaggcg ttgcgcgagc ggttcaacga catcaggggc ccccgctccg
     2761 acgacatctg ctacgcgacg cagaaccgcc aggtcgcggt gaaggcgatc gcccccgagg
     2821 ccgacgtcgt gctgatcgtc ggcgcgatca actccagcaa ctcgaaccgt ctcgtcgagg
     2881 tagcccgggt cgctggtacc ccgggctacc tcgttcccga cgagacctac ctcgaccccg
     2941 cgtggctcga gggggccgag accgtaggcg tgagctcagg agcaagtgcg ccggaagtgc
     3001 tggtggaacg gctgctcgca aagctcgcgg acctcggcta ccgccaagtc gagactcgtg
     3061 aggtgacgac ggaagacgtc acgttctcgc tgcctccctg gctgcgggaa gagcgatccg
     3121 ccgtcaccgg ctccggctga tcagcagcac ggcatgccgc cgaggattgc cagcacgtcg
     3181 tccatgggcc agtcggctcc tgccaccgtc cacagggcgg ccaacgctgc gaagacccga
     3241 acgatcttct tcacgcgagt catatgcaca cctcctttcg cgggctcgta ggggtcgagg
     3301 gaaggagatg taacgcccgc gttacctgta cggcatgagg tggcttacgc taacgttcgg
     3361 ccgcttgact cggagggtgc ttggtctgcg cgttagactg cgcaaggcgg ctgtgacagg
     3421 agggctatta ctcggcgatg agcgtcggac aacgtcttag acgactgcga ctccagagcg
     3481 ggctgaccca gaaagagctg gcggaccccc gctatacgca tgcctacgtc agcacgatcg
     3541 aggcggggcg gcgaatgcct tcggcgactg cgctcgagca cttcgcgagc aagctctcgg
     3601 tcgacgtcga agagctgcga acggggcgtc ccccggacct cgagcccaag ctccggctga
     3661 agctccaggg cgcacgccgg gacatctccg cgggtgatct cgaagaggcc gccaaggctc
     3721 tgacccaggt cttgcgcgac tccaagcgct acaagatgag ccatttggag gcgaaggccg
     3781 tcgaatacca ggctctcgtc gaagaacgcc gcgggaacat gcaggcggcg ttcgagatct
     3841 accaggacgt cgagaagatc ctgatcgagg ccccgccgcc gatgctcgcc gactgcatgg
     3901 cgggccaggc ccgctgcctg atcggcctcg gggacatgcg atacgcgatc cacctcgtgg
     3961 agagcttggt cgaacgtatg gagcgggagg gtttcaggga ccccaacgcg atggtccgcc
     4021 tgtacgcccc gctcgtctgg gcgtacgcgc agctcggggt ccagtcgaag gccagcgagt
     4081 acgcgggccg cgctctggag ctgctcccga aggtcaccga cccgttcaac ctcgccgtca
     4141 tgcaggtgaa cctcgccgag gcctacctca acgagggccg gatcaaggag gccgacgcag
     4201 cgctcctgcg agcggaggac ctcttcgaga acctcgagct ccgctccgag agcgccgccg
     4261 tccacatgaa tcgcgggtac tcgctcgtgc gcggcggggg cgacctcaag atcgcccgca
     4321 cagagctcga gaaggcactg gagctctacg tgtcgatggg cgacgagcgg ggcgaggccc
     4381 acgcccgcaa cgagctggcg cacgtggccc gcgtcgaagg agacgtcacc gaggcccgga
     4441 agcagcttga ccggtcgctc aagctcctca agaacagcga ggacgtcggt gagctagcgc
     4501 tcgcgtatct ggagctcggc ctcaccatca aggaggacga tcccaagaag ggcgagaa
//
LOCUS       DASQDO010000083         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_193002, whole genome shotgun sequence.
ACCESSION   DASQDO010000083 DASQDO010000000
VERSION     DASQDO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193002"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VEV43_02595"
     CDS             complement(<1..1226)
                     /locus_tag="VEV43_02595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYP22438.1"
                     /translation="MTPGVRASGAVRAALRGFEPTAEQWRAIAHPLEPVYLIAGAGSG
                     KTAVMAARIAWALEGHDLTPGNVLGLTFTNKAADELRERVALALDELHDETNEDVTVQ
                     TYNAFAAGIVRDHGLLVGVEPDAGLLSQAQQWQLVLSCLDDLPPFDALEVRSSYVVGQ
                     TLSLAHSVADHMASLNDIEAAAQRIRSMSGVDDKIALTAAKRQELVRAVRAYVAAKRR
                     TERIDFGDQVTKAVQVLEGDREIRDSYRRRFPFVLLDEYQDTNVAQRKLLQALVGSGG
                     AVTAVGDARQAIFAWRGATMYNLIGFPADFPRSDGAPYEPVSLSENFRSGSRILEVAN
                     RVVEAIDPSRRPGDPLRANARNGEGRVRAGLFSDERAEATWIAEQCEGLHGQVLGDGR
                     DPVEWKDIAILVRRKAA"
     gene            complement(1223..>2167)
                     /locus_tag="VEV43_02600"
     CDS             complement(1223..>2167)
                     /locus_tag="VEV43_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYP22439.1"
                     /translation="RAERIAALASVARICELDPGCSPAHPSEWWWRWDWTDGALSIRE
                     QQRAGESDLPLDKLRTSYSRISTYDNCGLQYLGSVVLGLDPDSSHNMAFGSVVHKVFE
                     ELETGELPLDAQAAYARYEVLFAEIKARFPNKATARQFHREGQLMIERYGRFLKPGAA
                     AVAEASFKVDLDGHRITGRIDRVDRIGKNIVISDYKTSKSVASWEEARESLQLAIYYL
                     AATTPGSVVEPPDVDMTALGEPAAMQLVYPNVPPVNGNVQKRSQKPEEAREVLKRLPA
                     LIDGVLAEDFRPNPEADCKWCKFKPLCPLWSEGKELPA"
BASE COUNT          297 a          777 c          751 g          342 t
ORIGIN      
        1 gcggccttgc gccgcacgag gatcgcgatg tccttccact cgaccgggtc ccgcccgtcg
       61 ccgaggacct ggccgtgcaa gccctcgcac tgctccgcga tccacgtcgc ctccgcccgc
      121 tcgtcggaga acagccccgc cctgaccctg ccctcgccgt tgcgggcgtt cgcgcgcagc
      181 gggtctccag gccggcgcga cgggtcgatc gcctcgacca cccggttcgc cacctcgagg
      241 atgcggctcc ccgaccggaa gttctcggac agggacacgg gctcgtaagg ggcaccgtcg
      301 gaccggggga agtcggccgg gaacccgatc aggttgtaca tcgtggctcc ccgccaggcg
      361 aagatcgcct gccgcgcgtc accgaccgcg gtgaccgcgc cgccggagcc gacgagagcc
      421 tgcaacagct tgcgctgcgc gacgttcgtg tcctggtact cgtcgagcag cacgaacggg
      481 aaccgcctcc ggtacgagtc gcggatctcg cggtcgccct cgaggacctg gaccgccttc
      541 gtcacctggt cgccgaagtc gatgcgctcg gtccgtcgct tggcggcgac gtacgcacgc
      601 acggcccgca cgagctcctg tcgtttcgcc gccgtcaacg cgatcttgtc gtcgacgccg
      661 ctcatggagc ggatcctctg ggccgcggcc tcgatgtcgt tgaggctcgc catgtggtcg
      721 gcgaccgagt gcgcgagcga cagcgtctgg ccgacgacgt acgaggaccg cacctcgagc
      781 gcgtcgaacg gcgggaggtc gtcgaggcac gacaggacga gctgccactg ctgggcctga
      841 ctcagcagtc cggcgtcggg ctcgacgccg acgaggagac cgtggtcgcg cacgatcccg
      901 gccgcgaacg cgttgtaggt ctggaccgtc acgtcctcgt tcgtctcgtc gtggagctcg
      961 tcgagcgcga gcgcgacccg ttcgcggagc tcgtccgccg ccttgttcgt gaacgtcagg
     1021 ccgaggacgt tccccggggt gaggtcgtgg ccttcgagag cccacgcgat ccgcgccgcc
     1081 atcacggccg tcttccccga tccggccccc gcgatcaggt aaacgggctc gagcggatgc
     1141 gcgatcgccc gccactgctc ggcggtcggc tcgaagccgc gcaacgccgc gcgcacggcg
     1201 cccgaagcgc gcacgccggg cgtcatgccg gcagctcctt gccttcggac cacagcgggc
     1261 acagcggctt gaacttgcac cacttgcagt cggcctcggg attcgggcgg aagtcctcgg
     1321 cgagcacgcc gtcgatgagc gccggcagcc gcttcagcac ctcccgcgcc tcctcgggct
     1381 tctggctgcg cttctggacg ttgccgttga ccggcgggac gttcgggtac acgagctgca
     1441 tcgccgcggg ctcgccgagc gcggtcatgt cgacgtcggg gggctcgacc accgacccgg
     1501 gcgtcgtcgc ggccaggtag tagatcgcga gctgcagcga ctcgcgcgcc tcctcccagc
     1561 tcgcgacgct cttcgacgtc ttgtagtcgg agatcacgat gttcttgccg atcctgtcga
     1621 cgcggtcgat gcggccggtg atgcggtgcc cgtcgaggtc gaccttgaag ctcgcctcgg
     1681 cgaccgccgc ggcccccggc ttgaggaacc tgccgtagcg ctcgatcatc agctggccct
     1741 cacggtggaa ctggcgtgcc gtcgccttgt tcgggaagcg ggccttgatc tcggcgaaca
     1801 gcacctcgta gcgcgcgtac gcggcttggg cgtcgagcgg gagctcaccg gtctcgagct
     1861 cctcgaagac cttgtgcacg acggacccga acgccatgtt gtggctcgag tcgggatcga
     1921 gaccgagaac gaccgagccg aggtactgga gcccgcagtt gtcgtaggtc gagatgcgcg
     1981 agtagctcgt gcgcagcttg tcgaggggga ggtcggactc gccggcccgc tgctgctcgc
     2041 ggatcgagag cgcgccgtcg gtccagtccc atcgccacca ccactcgctc gggtgcgcgg
     2101 gcgagcagcc cgggtcgagc tcgcagatgc gcgccacgga tgcgagggcc gcgatccgct
     2161 cggcccg
//
LOCUS       DASQDO010000084         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_560015, whole genome shotgun sequence.
ACCESSION   DASQDO010000084 DASQDO010000000
VERSION     DASQDO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560015"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VEV43_02605"
     CDS             complement(<1..616)
                     /locus_tag="VEV43_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22440.1"
                     /translation="MKLVRGLAAAVTLVALVTPVGANAQLPLLGDKCFGDDVTIAGTE
                     EGDVLEGTPGDDVISALAGDDVVRGGGGNDTICGGAGADSIEGGAGRDAVAVGRGADD
                     ARGGEGSDIIVEFSSDSCSGCLPFDTAARDASDDVISGGEGNDAVGAEGGNDTIDGGE
                     GQDLSLFLSRFTTVSIDLSLGRALSTAGGLDELRGIEHVLGSVNA"
     gene            complement(681..>2103)
                     /locus_tag="VEV43_02610"
     CDS             complement(681..>2103)
                     /locus_tag="VEV43_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYP22441.1"
                     /translation="LGLIEARGMVELDADGDVVKVAGTVQDVTDRKSLEDRIQHQAFH
                     DSLTGLANRDLFVDRVGHALSRQGRVARPLAVLFLDLDDFKTVNDSLGHRAGDDLLVD
                     VARRISGAIRPGDTVARLGGDELAILLEDLEGVDGAVFVADRLNELFDDPFLVDDSEL
                     SVHASIGIAYCSPGEGRSPDDLLRDADIAMYEAKRSGKATFQLFESGMRVAATDRLRL
                     KADLQQAVDNGELELHCQPIVRLVDGSVTSVEALVRWRHPERGLVPPLEFIPFAEDSG
                     LIVPIGRWVLFEACRTAASWAGPNPPSVSVNVSPLRFRHPGFVEELTEALEATGLPAE
                     RLVLEITESALVEDVDKVVERLDDLKRLGVQLAIDDFGTGYSSLSYLKHFAVDVLKID
                     KAFIDSIALGAEDSALARAVLKLGQSLNLKIVAEGVEEGAQADVLRSLGCPLAQGYLF
                     SRPVPADALAELLEGRETAAPAA"
BASE COUNT          318 a          765 c          700 g          320 t
ORIGIN      
        1 cggcgttgac cgacccgagc acgtgctcga tcccgcgcag ctcgtccagg cctcccgcgg
       61 tcgagagagc gcgcccgaga gacaggtcga tcgagacggt cgtgaagcgc gacaggaaca
      121 gcgacaggtc ctgaccctct ccgccgtcga tcgtgtcgtt gccgccctcg gcgccgaccg
      181 cgtcgttgcc ctctccaccg gagatgacgt cgtcgctcgc gtcgcgcgcg gccgtgtcga
      241 agggaaggca cccggagcag ctgtcggagg agaactcgac gatgatgtcg gacccttcgc
      301 cgccgcgtgc atcgtccgct ccgcgtccga cggctacggc gtcgcgtccg gcgcctccct
      361 cgatcgagtc cgccccggct ccgccgcaga tggtgtcgtt cccgccgcca ccgcggacga
      421 cgtcgtctcc cgcaagcgcg gagatcacgt cgtctccggg agttccctcg aggacgtcgc
      481 cctcttcggt gccggcgatc gtcacgtcgt cgccgaagca cttgtcgccg aggagcggga
      541 gctgggcgtt cgctcccacc ggagtcacga gggcgacgag cgtcacggcc gccgccaggc
      601 cgcgcacgag tttcacgtgg aaagtcttcg acccgccctg ccgaaccacc ttcccggcct
      661 cgcggggcgg gcgctcggcc tcaggcagca ggggccgcgg tctcccgtcc ttccagcagc
      721 tccgccaggg cgtccgccgg gacggggcgc gagaacaggt agccctgtgc cagcgggcag
      781 cccaggctcc gcagcacgtc cgcctgggct ccttcttcca cgccctcggc gacgatcttg
      841 aggttcagcg actgccccag cttcagcacg gcccgggcca gggccgagtc ctcggccccc
      901 agcgcgatcg agtcgatgaa cgctttgtcg atcttcagca cgtcgaccgc gaagtgcttg
      961 aggtagctga gcgacgaata gcccgtgccg aagtcgtcta tcgcgagctg tacgcccaga
     1021 cgcttcagat cgtccaggcg ctcgacgacc ttgtccacat cctccaccag cgcgctctcg
     1081 gtgatctcga gcaccaggcg ctccgccggg aggccggtcg cctccaaggc ttcggtcagc
     1141 tcttccacga agcccggatg gcggaagcgc agcggcgaca cgttgaccga taccgacgga
     1201 ggattcggcc cggcccacga cgccgccgta cggcacgcct cgaacaggac ccatctcccg
     1261 atggggacga tcaagccgct atcttcggcg aacgggatga actcgagcgg agggacgagg
     1321 ccgcgctcgg gatggcgcca ccgcacgagc gcctcgacgg acgtgacgct gccgtcgacg
     1381 agccggacga tcggctggca gtggagctcg agctcgccgt tgtccaccgc ctgctgcaag
     1441 tcggccttga gtcgcaggcg gtccgtcgcg gcgacgcgca tcccggactc gaacagctgg
     1501 aacgtcgcct tgccgctccg cttcgcctcg tacatggcga tgtccgcgtc gcgcaggagg
     1561 tcgtcgggcg aacgtccctc gcccggcgag cagtacgcga tgccgatgct cgcgtggacg
     1621 gacagctcgg agtcgtcgac gaggaacggg tcgtcgaaca gctcgttgag ccggtcggcg
     1681 acgaagaccg cgccgtcgac gccttctagg tcctcgagca ggatcgcgag ctcgtcgccc
     1741 ccgagccgcg cgacggtgtc gcccggcctg atggccccgc tgatccggcg cgcgacgtcg
     1801 accagcagat cgtccccggc tcgatgcccc aggctgtcgt tgacggtctt gaagtcgtcg
     1861 aggtccagga acagcaccgc gagaggacgc gcgacgcgcc cctgacggga caaggcgtga
     1921 ccgactcggt cgacgaacag gtcccggttc gctagccccg tgagcgagtc gtggaacgcc
     1981 tgatgctgga tcctgtcttc gagcgacttc cggtccgtga cgtcctggac ggtccccgcg
     2041 accttgacga cgtcgccgtc cgcgtcgagc tcgaccatcc cgcgcgcttc tatgagaccc
     2101 agc
//
LOCUS       DASQDO010000085         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_437740, whole genome shotgun sequence.
ACCESSION   DASQDO010000085 DASQDO010000000
VERSION     DASQDO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437740"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VEV43_02615"
     CDS             <1..1750
                     /locus_tag="VEV43_02615"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22442.1"
                     /translation="VPAPGDAPDGGFVTQSEAELGLEKWENIYDINRSYFGIEPNPKS
                     PAQNLPPGLPSDWRDADCDPRIHIVNFNMDTPGSSGSYTAGYYSSEHEYPNGEGEHES
                     IYSNEAEMFFMNSLHLNVGDDTYAGVLAHEFFHMIQFSNDYNEETWVNEGMADVAAVV
                     NGFGDIVQGHIDAYQENPDQHLFDWTGVVDDYGQAFLFFDYLFNHYGAPEVEGTDELE
                     AYGLAKLLTRTPADGAKGITQVIATRPDSLKSSLDRYYRKGTFGKVFKDYLIANYLDD
                     PSLARGQFGYANRDVAVAGTGTEDASPADSTVHPYGADYYEVTGEGTFKSKVQDPVAV
                     IPATEGQPGPGKKFAWSNRADEMITWLERGANLTDATAPALTFDYWHQIEEDWDYAYV
                     RISQDRGASYVFVPTPDCGGRTTNPNGNNRAATEGGGITGDSEGWKTCSLDLTQWAGG
                     PVLIRFEYDTDQATTEPGFVIDNVALKDGDRTIWRKETFEKRARKFDFGGDALKKWIA
                     LAPLKKNRPLVQVVQVAGKSAIRYTLTRKGYRKVKAGLRQRRATGVRGDKTIMVFSGT
                     TPIATTPFGYSYKVNR"
     gene            complement(1817..>2592)
                     /locus_tag="VEV43_02620"
     CDS             complement(1817..>2592)
                     /locus_tag="VEV43_02620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP22443.1"
                     /translation="EEAENRSPRRSVERRGVKPHPREAYFEAVGDLLGSSYLRYSFTK
                     GTDQEVAFLLELLGLSPGARVLDVGCGPGRHAVPLAQAGFGVTGVDVSRRFLDLAAES
                     ARAAGVGASFFQVDARQMPFDDEFDAVISICEGAFGLMAGDDSTVLKRMAEAVRRGAR
                     VVLTAFSALYEASHPREGATFDADAGVVHELTTIKDESGADHEVDLWTSVYTPRELRL
                     MALGTGLIPEQVWSVEPGAFAKTEPNLDLPELMLVARRP"
BASE COUNT          468 a          903 c          858 g          363 t
ORIGIN      
        1 cgtcccggcc cccggcgacg cgccggacgg cgggttcgtc acgcagagcg aggccgagct
       61 cggtctcgag aagtgggaga acatctacga catcaaccgc tcgtacttcg gtatcgagcc
      121 gaacccgaag tcgccggcgc agaacctccc gccgggcctt ccctccgact ggcgcgacgc
      181 ggactgcgac ccgcgcatcc acatcgtcaa cttcaacatg gacacccccg gctcgtccgg
      241 tagctacacc gccggttact actcctcgga gcacgagtac ccgaacggcg aaggcgagca
      301 cgagtcgatc tactcgaacg aggcagagat gttcttcatg aactcgctgc acctgaacgt
      361 cggcgacgac acgtacgcag gtgtgctcgc gcacgagttc ttccacatga tccagttctc
      421 gaacgactac aacgaggaga cctgggtcaa cgaaggcatg gccgacgtcg ccgccgtcgt
      481 gaacggcttc ggcgacatcg tgcagggcca catcgacgcc tatcaggaga accccgacca
      541 gcacctgttc gactggaccg gcgtcgtcga cgactacggc caggcgttct tgttcttcga
      601 ctacctcttc aaccactacg gggcccccga ggtggagggc accgacgagc tcgaggcgta
      661 cgggctcgcg aagctcctga cgcgcacgcc ggccgacggc gccaagggga tcacgcaggt
      721 gatcgcgacg cggcccgact cactcaagtc gtcgctcgac cgctactacc gcaagggcac
      781 gttcgggaag gtcttcaagg actacctgat cgcgaactac ctcgacgacc cgagcctcgc
      841 tcgcgggcag ttcggctacg cgaaccgcga cgtcgccgtg gcggggaccg ggaccgagga
      901 cgcgtcgccg gcggactcga ccgtccaccc gtacggcgcc gactactacg aggtgacggg
      961 cgaggggacg ttcaagagca aggtgcagga tcccgtcgcg gtcatccccg cgaccgaggg
     1021 ccagccgggg ccgggcaaga agttcgcctg gtcgaaccgg gccgacgaga tgatcacgtg
     1081 gctcgagcgc ggcgcgaacc tcaccgacgc gaccgcgccg gcgctgacgt tcgactactg
     1141 gcaccagatc gaagaggact gggactacgc gtacgtgcgg atctcgcagg accgcggtgc
     1201 gtcatacgtc ttcgtgccta cgccggactg cggtgggcgc acgacgaacc cgaacggcaa
     1261 caaccgggcc gcaaccgagg gcgggggcat caccggtgac tcggagggct ggaagacgtg
     1321 ctcgctcgac ctgacgcaat gggcgggggg gccggtgctg atccggttcg agtacgacac
     1381 cgaccaggcg acgaccgagc ccggcttcgt catcgacaac gtggcgctca aggacggcga
     1441 ccggacgatc tggcgcaagg agacgttcga gaagagggcc cggaagttcg acttcggggg
     1501 tgacgcgctg aagaagtgga tcgcgctggc gccgctgaag aagaaccgtc cgctggtcca
     1561 ggtggtccag gtcgcgggca agagcgcgat ccgctacacg ctgacgcgga agggctaccg
     1621 caaggtgaag gcggggctgc gccagcgccg ggccacgggg gtccggggcg acaagacgat
     1681 catggtgttc tcgggcacga cgccaatagc gacgacgccg ttcggctaca gctacaaggt
     1741 gaaccgctag cccaagcctc tcgagtgcgc agccggttac ggaatccgta gctcgttgcg
     1801 cactcgacgg ggcgcgtcag gggcggcggg ctaccagcat cagctccggc aggtccaggt
     1861 tcggctccgt cttcgcgaac gcgcccggct ctaccgacca cacttgctcc gggatcaggc
     1921 ccgtgcccag cgccatcagc ctcagctcgc gcggcgtgta gacgctcgtc cataggtcta
     1981 cctcgtggtc ggcccccgac tcgtccttga tcgtcgtcag ctcgtggacc acgcccgcgt
     2041 cggcgtcgaa cgtcgcgcct tcgcgcgggt gcgaggcctc gtacagcgcg ctgaacgccg
     2101 tcagcaccac gcgggccccc cggcgcacgg cttcggccat gcgcttcaac accgtcgagt
     2161 cgtcgcccgc catcaggccg aacgcgccct cgcagatcga gatcaccgcg tcgaactcgt
     2221 cgtcgaacgg catctgccgc gcgtcgacct ggaagaacga cgcgcccacg ccggcggcgc
     2281 gggccgactc cgcagcaagg tccaggaacc ggcgcgacac gtcgacgccc gtcaccccga
     2341 accccgcctg cgcgagcgga accgcgtggc gtcccggccc gcagccgacg tccaggacgc
     2401 gggcgcccgg cgacaacccc aacagctcca gcaggaacgc gacttcctgg tccgtcccct
     2461 tcgtgaagct gtagcgcagg tacgacgagc cgagcaggtc gccgaccgcc tcgaagtagg
     2521 cctcgcgggg gtggggcttc actcctctgc gctcgaccga tcgccgaggc gatcggtttt
     2581 cggcttcttc tc
//
LOCUS       DASQDO010000086         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1049962, whole genome shotgun sequence.
ACCESSION   DASQDO010000086 DASQDO010000000
VERSION     DASQDO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049962"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VEV43_02625"
     CDS             complement(<1..379)
                     /locus_tag="VEV43_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYP22444.1"
                     /translation="MRQTLRVSIDEFLEPRIIIVSGKGGVGKTTVAAALALVAARHGR
                     KVCIAEVDRKGTLPRLFGGPTLGYEPRQLSPGVFGMNIVPEDALAEYLDVQYHMKRIS
                     KVFTSSHFVDYITAAAPGLKDILM"
     gene            598..918
                     /locus_tag="VEV43_02630"
     CDS             598..918
                     /locus_tag="VEV43_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22445.1"
                     /translation="MILSLWLWDEAWLVPTVTGSLAVLSLGARRLRWARRIDRERLLP
                     AGPTGDPQNEAWRRIMSREAWEEAFAWLGWEPSAGATTRWLIYRFAFVALAAAPLASS
                     LREG"
     gene            961..2325
                     /locus_tag="VEV43_02635"
     CDS             961..2325
                     /locus_tag="VEV43_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HYP22446.1"
                     /translation="MARSARRLSVLALVLGALGLGLGPASAQAPGTATVDVVEVDGVI
                     DGPTAEYLIDKIDAAQEDAVAIVLQLDTPGGLDIAMRDVIGGIMQSEIPVIVWVAPQG
                     SRAASAGTFIAYSAHLTYMAAATELGAASPVNLAGGDLPPTVKEKATNDAVAFLTELA
                     RERGRDVEFAEAAVRDAEAIGVTEAIERGVVDGQASSLSELLQLVDGQTVDVTGGSAT
                     LDTWDPAAGKLTVTLRFQEMDLLQRLQHALTSPEVAFLMLLAGVFGLIFELYNPGIGL
                     AGILGAGSLAMGFYGLSVLPTNWLGVLAVVVAIVLLLVDLHTGGLGGWSIAGLGLLVV
                     GGLMTFAGANEALALSPWAIVAAVLFTLLFFVSVMTAALRVRLRRPITGEEAIVGAVG
                     EAKTDIAPEGTVVTKGTLWRARTMETGIAAGSKVQVKATEGLVLLVEPLHEQEPVGSA
                     NKEG"
     gene            2326..3162
                     /locus_tag="VEV43_02640"
     CDS             2326..3162
                     /locus_tag="VEV43_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HYP22447.1"
                     /translation="MTLGVLGVVAVLVLIIAAASIRVVQEYERGVIFRLGRVVAGAKG
                     PGLFFIVPIIDKMVKVNLQIVTLDVPPQDVITKDNVTVRVNAVCYFQVFQPVKAIVEV
                     QNYLYATSQIAQTTLRAVLSKVDLDTILGERDRLNSDLQRIIDDLTDPWGVKVNLVEI
                     KDVDLPVEMQRAMARQAEAERGRRAEIIDAEGEFQASEKLAQAAEVIARHPIAYQLRY
                     LQTITDVSAEKNSTLVVPIPIELLAPFKAAFGKDLSAFDPADGAPPPATLHPVEQPPQ
                     TP"
     gene            complement(3129..3512)
                     /locus_tag="VEV43_02645"
     CDS             complement(3129..3512)
                     /locus_tag="VEV43_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYP22448.1"
                     /translation="MRATRQRAAVRDALDRSGGFRTAQALHDEMKAAGESVGLTTVYR
                     TLQSLADAGEVDVLRTSAGEAIYRRCATGEHHHHLVCRSCGASVEVASDEVERWAATT
                     AERHGFTEVEHTAELYGVCGGCSTG"
     gene            complement(3512..>3961)
                     /locus_tag="VEV43_02650"
     CDS             complement(3512..>3961)
                     /locus_tag="VEV43_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HYP22449.1"
                     /translation="DDVIHPVAISNLVGDGLHNLLDGSLIGASYLVSPRVGLATTIAV
                     VLHEIPQELGDFGILVHSGVEPKKALRLNLLTGSMAVIGAVLALLSGDVLSEQLLLPV
                     AAGAFIYIASTDLLPELHKEPEPRKSLFQICALIAGVGVMVALLALD"
BASE COUNT          592 a         1344 c         1414 g          611 t
ORIGIN      
        1 gcatgaggat gtccttcagg ccgggggccg cggccgtgat gtagtcgacg aagtgcgagc
       61 tcgtgaagac cttgctgatc cgcttcatgt ggtactggac gtcgaggtat tcggcgaggg
      121 cgtcctccgg cacgatgttc atcccgaaga cgccgggcga gagctggcgc ggctcgtagc
      181 cgagcgtcgg cccgccgaac aggcgcggca gcgtgccctt gcggtcgacc tccgcgatgc
      241 agaccttgcg gccgtggcgc gcggcgacga gcgcgagcgc ggctgcgacc gtggtcttgc
      301 cgacgccgcc cttgccggac acgatgatga tccgcggctc gaggaactca tctatcgaca
      361 cccgcagagt ctgtcgcatc ggggacaccg gaacacacgt tcgaatgggc cccggtgtgg
      421 gtatcgacga cggcagtcga ttcaaggagg tgcggtcgat ggcggagcaa caatcgaacc
      481 tgcagaagga cccggacgac ggccgccgga gctgacccgg gccacttcca ctcgcggcga
      541 gacgccgcgt cgttcgtcgc cgcgcagctg accttcttcg ggtggttccc ggcgtcgatg
      601 atcctgtccc tgtggctttg ggacgaggct tggctcgtac cgacggtcac gggttcgctg
      661 gcggtcctgt cgctcggtgc ccgacgtctg cgctgggcgc gccggatcga tcgcgagcgc
      721 ctgttgccgg ccgggcccac gggtgatccg cagaacgagg cctggcgccg gatcatgagc
      781 cgcgaggcat gggaagaggc cttcgcctgg ctcggctggg aacctagcgc cggcgccacc
      841 acgcgatggc tgatctacag gttcgcgttc gtggccctgg ctgcagcccc cctggcctcg
      901 tccctgcggg aaggctgacg cggcgaagca aagcggacga atccctgcca ccatggggac
      961 atggctcgca gcgcgcggcg gctctcggta ctggccctcg tcctcggcgc gctcgggctc
     1021 gggctcggcc ccgcttccgc ccaggccccc ggcaccgcga ccgtcgacgt cgtcgaggtc
     1081 gacggcgtca tcgacgggcc gaccgccgag tacctgatcg acaagatcga cgcggcgcaa
     1141 gaggacgcgg tcgcgatcgt cctccagctc gacacccccg gcgggctcga catcgccatg
     1201 cgcgacgtca tcggcgggat catgcagtcg gagatccccg tgatcgtctg ggtcgcgccg
     1261 cagggctcga gggccgcgtc ggccgggacg ttcatcgcct actccgcgca cctcacctac
     1321 atggccgcgg cgacggagct gggggccgcg tccccggtga acctcgccgg cggcgacctg
     1381 cctcccaccg tcaaggagaa ggcgaccaac gacgcggtcg cgttcctcac cgagctggcg
     1441 cgcgagcgcg gacgcgacgt cgagttcgcg gaagcggcgg tgcgcgacgc cgaggcgatc
     1501 ggcgtgaccg aggcgatcga gcgcggggtc gtcgacgggc aggcgtcgtc tctcagcgag
     1561 ttgctccagc tcgtcgacgg ccagacggtc gacgtcaccg ggggatcggc cacgctcgac
     1621 acgtgggacc ccgcggccgg caagctgacc gtgacgctgc ggttccagga gatggacctg
     1681 ttgcagcggc tgcagcacgc gctcacgagc cccgaggtcg cgttcctgat gctgctcgcc
     1741 ggcgtcttcg ggctgatctt cgagctctac aaccccggca tcgggctcgc cggcatcctc
     1801 ggcgcgggct cgctcgcgat ggggttctac gggctgagcg tcctcccgac gaactggctt
     1861 ggcgtcctcg cggtcgtcgt cgcgatcgtg ctgctgctcg tcgacctgca taccggcggc
     1921 ctcggcggct ggagcatcgc gggcctcggc ctcctcgtcg tgggcggcct tatgacgttc
     1981 gccggcgcga acgaggccct cgcgctgagc ccgtgggcga tcgtcgccgc agtcctgttc
     2041 acgttgctgt tcttcgtctc ggtcatgacc gccgcgctga gggtgcggct gcgccgcccg
     2101 atcaccgggg aagaggcgat cgtcggcgcg gtcggcgagg cgaagaccga catcgcgccc
     2161 gaggggaccg tcgtcaccaa ggggacactg tggcgggcgc ggacgatgga gactggcatc
     2221 gcggcgggtt cgaaggtgca agtgaaggcg accgaaggtc tcgtcctgct cgtggagccg
     2281 ctccacgagc aggagccagt gggttcggcg aacaaggagg gttagatgac tctgggtgtc
     2341 ctcggcgtgg tggcggttct ggtcctgatc atcgcggcgg cttccatccg ggtggtccag
     2401 gagtacgagc ggggcgtgat cttccggctc gggcgggtcg tcgccggcgc caaggggccg
     2461 ggtctgttct tcatcgtccc gatcatcgac aagatggtga aggtcaacct ccagatcgtg
     2521 acgctcgacg tcccgccgca agacgtcatc acgaaggaca acgtcacggt gcgcgtgaac
     2581 gccgtgtgct acttccaggt cttccagccg gtgaaggcga tcgtcgaggt ccagaactac
     2641 ctctacgcga cctcgcagat cgcgcagacg acgttgcggg ccgtgctgtc gaaggtcgac
     2701 ctcgacacga tcctcggcga gcgcgaccgt ctgaacagcg acctccagcg gatcatcgac
     2761 gacctgacgg acccgtgggg cgtcaaggtg aacctcgtcg agatcaagga cgtcgacctc
     2821 cccgtcgaga tgcaacgtgc gatggcgcgc caggccgagg ccgagcgagg ccggcgcgcg
     2881 gagatcatcg acgccgaggg cgagttccag gcgtctgaga agctggcgca ggccgccgag
     2941 gtgatcgcgc ggcacccgat cgcctaccag ctccgctacc tgcagacgat caccgacgtg
     3001 tcggcggaga agaactcgac gctcgtcgtg ccgatcccga tcgagctgct cgcgccgttc
     3061 aaggcggcgt tcggcaagga cttgtcggcg ttcgacccgg ccgacggagc cccgccgccg
     3121 gccacgcttc accccgtgga gcagcctccg cagaccccgt agagctccgc ggtgtgctcc
     3181 acctcggtaa acccgtggcg ctccgcggtc gtcgccgccc agcgctcgac ctcgtcgctc
     3241 gcgacctcga ccgacgcgcc acaggagcgg cacacgaggt ggtggtggtg ctcgccggtc
     3301 gcgcaacggc ggtagatcgc ctcgcccgcc gaggtgcgca gcacgtcgac ctcgcccgcg
     3361 tcggcgaggc tctgtagcgt ccggtagacc gtcgtgagtc cgacgctctc gccggcggcc
     3421 ttcatctcgt cgtgcagggc ctgcgcggta cggaagcccc cggagcggtc gagggcgtcg
     3481 cgcacggccg cgcgctgccg cgtcgcgcgc atcagtcgag cgcgagcagg gcgaccatga
     3541 ccccgacgcc cgcgatcagc gcacagatct ggaacagcga cttgcgcggc tccggctcct
     3601 tgtgcagctc cggcaacagg tcggtgctcg cgatgtagat gaacgcgccg gcggcgaccg
     3661 gcaacaagag ctgctcggag agcacgtcgc ccgagagcag cgccaggacg gcgcctatca
     3721 ccgccatcga gccggtcagc aggttgagcc gcagggcctt cttcggctcg accccggagt
     3781 ggacgaggat cccgaagtcg ccgagctcct gcgggatctc gtggaggacg acggcgatcg
     3841 tcgtggcgag cccgaccctc ggcgagacga ggtagctcgc tccgatcagc gagccgtcga
     3901 ggaggttgtg gagcccgtcg ccgacgaggt tcgagatcgc gaccggatgg atgacgtcgt
     3961 c
//
LOCUS       DASQDO010000087         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_682475, whole genome shotgun sequence.
ACCESSION   DASQDO010000087 DASQDO010000000
VERSION     DASQDO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682475"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VEV43_02655"
     CDS             <1..944
                     /locus_tag="VEV43_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22450.1"
                     /translation="AFPLASYLAMAWMTHELGVPGFVAVLLAVDAVLVGLAFVALRSP
                     LNRLAAVAAATLVVMVGDLVLGERLQLNAVFGNSPIVAGRFAGLGNLAFSILGASAVV
                     LATVLVHKHERSARALAGAAAVFALAVVVDGAPFLGSDVGGVLALVPGFAITWFLLSG
                     RKVGVRAVLLAGLAGVVALGLFLAVDLSRPEDSRTHLARLVENVADEGPQVFADTIER
                     KVRTNVRIFGSTIWTYLVPPALLLLGVLLRKPPGRWQQLATAFPVVRSGLVGGLVLAL
                     LGFAVNDSGIVVPAMFLSWLVPMALIAYVSLERRGAA"
     gene            941..1756
                     /locus_tag="VEV43_02660"
     CDS             941..1756
                     /locus_tag="VEV43_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22451.1"
                     /translation="MNDLLWALAAAGTGALVALAFRAPALASPPPWSLRTNVSGRQVP
                     AVLGVGVVLGGAGGGLAGALGRGDGLGRLEAATAIAFLGLAAAGLWDDTRGDESPRGF
                     GGHLRAGRLTGGLVKIAAGGLAGIAAAALLYRDDLVMAALTALAVPLAANLFNLLDRA
                     PGRTGKVALLAGLPLLAMGDERWAVDAAGAFGALAALLPADLGERGMLGDAGANPLGA
                     LVGLGLAVSLDAAPLGAVVVVLLALNAASERWSFSKVIESSPPLRALDELGRK"
     gene            1883..3517
                     /locus_tag="VEV43_02665"
     CDS             1883..3517
                     /locus_tag="VEV43_02665"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYP22452.1"
                     /translation="MAKHVFVTGGVVSSLGKGLTAASLGRLLKNRGLRVMMQKLDPYI
                     NVDPGTMNPFQHGEVFVTEDGGETDLDLGHYERFIDENLMKASNITTGAIYQSVIAKE
                     RRGDFLGDTVQVIPHITNAIKQRIRSLAEDSGADVLITEIGGTVGDIESLPFLEAIRQ
                     LKTELGRDDTCYVHVTLVPYMGPAEELKTKPTQHSVRELRSIGIHPDAIVCRSDRPIS
                     PGLKRKISMLCDVGMDAIVSAVDSNNIYEIPFVLRDEGLDDFVIDRLRIEGTSAPDLS
                     GWRDLVARIDSAKESVRIGIIGKYVTLPDAYLSVVESLKHAAFSHGRQVDITWVASDD
                     LETGDPDEMLGELDGILIPGGFGVRGVEGKIRAVQYARENAIPFFGICLGLQCAVVEF
                     ARNVAGFEDANSSEFGNSDHPVVDILVGQDLDRLGGTMRLGAYLCKLAPGTKAEAAYG
                     KEVITERHRHRYEVNPRFRRRLEDAGLVCSGESPDGTLVEIIELPDHPWFVAGQFHPE
                     FKSRPDRPHPLFRDFVGAALAHSGADAAVDLTETRI"
     gene            complement(3551..>4353)
                     /locus_tag="VEV43_02670"
     CDS             complement(3551..>4353)
                     /locus_tag="VEV43_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22453.1"
                     /translation="AWAVGERGQTKVKTIVQRYDGTSWRMRPSLNPSADRNVLYGVDV
                     APSGDVYAAGTRFNAKRRFRTMVHHYDGTAWKMVAGRYPGVLWDVDVVAEDDVWAVGT
                     KSVDGRGRAFAVHFDGTGWTEVPTPSPGDGFSVFYGVSGSAPDDVWAVGEWYDRGDPR
                     AWAIHFDGTSWSAAELPDLGTFVTLRGVSALDADTAVAVGEDQHDDHDSRVVIEWDGT
                     SWSRAGQDDETQNNWPLAVDLAPDGAGWAVGYHSADPATEYIERRTCG"
BASE COUNT          564 a         1556 c         1532 g          701 t
ORIGIN      
        1 ccgcgttccc gctcgcgtcg tacctggcga tggcctggat gacgcacgag ctcggcgtcc
       61 cgggcttcgt cgccgtgctg ctcgcggtag acgccgttct cgtcgggctc gctttcgtgg
      121 cgctccggtc acctctgaac cggcttgcgg cggtagcggc cgcgaccctc gtcgtcatgg
      181 tcggcgacct cgtgctcgga gagaggctcc agctcaacgc ggtcttcggg aactcgccga
      241 tcgtcgcggg gcgcttcgcg gggctcggca acctcgcgtt ctcgatcctc ggcgcgtccg
      301 ccgtcgtgct ggcgaccgtg ctcgtccaca agcacgagcg gtcggcgcga gccctcgccg
      361 gggccgccgc cgtgttcgcg ctggccgtcg tcgtcgacgg tgcgccgttc ctcggcagcg
      421 acgtcggagg cgtgctcgcg ctcgtcccgg ggttcgcgat cacgtggttt ttgctgtcgg
      481 ggcgcaaggt cggggtgcgc gccgtgttgc tggccgggct cgccggcgtc gtcgcgctcg
      541 gcctcttcct cgccgtcgac ctgtcgcgcc ccgaggactc ccgcacccac ctcgcgcggc
      601 tcgtcgagaa cgtcgccgac gaggggccgc aggtcttcgc cgacacgatc gagcgcaagg
      661 tgcggacgaa cgtgcgcatc ttcggctcga cgatctggac gtacctcgtg ccgcccgcgc
      721 tgttgctcct cggggtgctg ctgcgcaagc cgccgggccg ctggcagcag ctggcgacgg
      781 cgttcccggt ggtgcgcagc ggcctggtcg ggggcctggt cctcgcgctg ctcgggttcg
      841 ctgtcaacga ctcggggatc gtggtgccgg cgatgttttt gtcgtggctc gtcccgatgg
      901 ccctgattgc gtacgtgtca ctggaacgcc ggggggccgc ttgaacgacc tcctgtgggc
      961 tctcgccgcg gcaggaaccg gggccctcgt agcgctcgcc ttccgggccc cggcgctggc
     1021 gtccccgccc ccgtggtcgc tccgcacgaa cgtgtccggc agacaggtcc ccgccgtcct
     1081 cggtgtgggc gtcgtcctgg gcggggccgg cgggggactg gccggtgcgc tcgggcgcgg
     1141 ggacgggctc ggccgcctgg aggccgccac cgccatcgcg ttcctcgggc tcgcggccgc
     1201 aggcctctgg gacgacacgc gcggcgacga gagcccccgc ggcttcggcg gacatctgcg
     1261 ggcggggcgc ctcaccggcg gtctcgtcaa gatcgcggcg ggggggctgg ccgggatcgc
     1321 cgcggcggcc ctcctctatc gggacgacct cgtgatggcg gcgctgaccg cgctcgcggt
     1381 gccgctcgcg gcgaacctct tcaacctgct cgaccgggcc cccggacgga ccggcaaggt
     1441 cgctctcctc gcggggctgc cgctgctggc gatgggcgac gagcgctggg ccgtcgacgc
     1501 ggccggggcg ttcggtgcgc ttgccgcact cctgccggcc gacctcggcg agcgcgggat
     1561 gctcggtgac gccggcgcga acccgctggg cgcactggtc gggctcggtc tggcggtctc
     1621 gctcgacgcc gccccgctgg gggccgtggt cgtcgtgctc ttggcgctca acgccgcctc
     1681 ggagaggtgg tcgttctcga aggtgatcga gtcgtcgccg ccccttcgcg ctttggacga
     1741 gctcggccgg aaatgagctc gcgcggcggg gccgcgtgct agcttgtgac gacttccccc
     1801 gaacctctca ggcggtcgac ccatgccggg ttccgggacg tcacctgcat ggcccgaagc
     1861 gcatcaaacg aagggagcgg tggtggcgaa gcacgttttc gtgacgggtg gggtggtctc
     1921 cagcctcggc aagggcctca ctgcagcgtc cctcggcagg ctcctcaaga accgcgggct
     1981 gcgcgtgatg atgcagaagc tcgaccccta catcaacgtc gaccccggca cgatgaaccc
     2041 cttccagcac ggcgaggtct tcgtgaccga ggacgggggc gagaccgacc tcgacctcgg
     2101 ccactacgag cgcttcatcg acgagaacct gatgaaggcg tcgaacatca cgacgggcgc
     2161 gatctaccag tcggtgatcg cgaaggagcg caggggcgat ttcctcggcg acaccgtcca
     2221 ggtgatcccg cacatcacga acgcgatcaa gcagcgcatc cgctcgctcg ccgaggactc
     2281 cggcgccgac gtgctgatca ccgagatcgg cgggaccgtc ggcgacatcg agtcgctccc
     2341 gttcctcgag gcgatccgcc agctgaagac cgagctcggc cgcgacgaca cttgctacgt
     2401 ccacgtgacg ctcgtgccgt acatgggccc cgcggaggag ctcaagacga agccgactca
     2461 gcactcggtg cgcgagctcc gcagcatcgg gatccacccg gacgcgatcg tgtgccgttc
     2521 ggaccgtccg atcagccccg ggctcaagcg gaagatctcg atgctttgcg acgtcggcat
     2581 ggacgcgatc gtctcggcgg tcgactccaa caacatctac gagattccgt tcgtgctgcg
     2641 cgacgagggc ctcgacgact tcgtgatcga ccgccttcgc atcgagggca cctcggcccc
     2701 cgacctgagc ggatggcgcg acctcgtggc gcgcatcgac tcggcgaagg agagcgtccg
     2761 gatcgggatc atcgggaagt acgtcacgct gcccgacgcc tacctgtcgg tggtcgagag
     2821 cctcaagcat gcagcgttct cgcacgggcg ccaggtcgac atcacatggg tcgcgtccga
     2881 cgacctcgag acaggcgatc ccgacgagat gctcggcgag ctcgacggca tcctgatccc
     2941 cggcggcttc ggggtgcggg gcgtcgaggg caagatccgc gccgtccagt acgcgcgcga
     3001 gaacgcgatc ccgttcttcg gcatctgcct cgggctccag tgcgcggtcg tcgagttcgc
     3061 acggaacgtc gcgggcttcg aggacgcgaa ctcgtccgag ttcggcaact ccgaccaccc
     3121 ggtcgtcgac atcctcgtcg gccaggatct cgatcgcctc ggggggacca tgcgtctcgg
     3181 cgcgtacctc tgcaagctcg cgccggggac gaaggccgag gccgcgtacg gcaaggaggt
     3241 catcaccgag cggcaccgtc accgctacga ggtgaacccg cgcttccgcc gcaggctcga
     3301 ggacgcgggg ctcgtgtgct cgggcgagtc gcccgacggg accttggtcg agatcatcga
     3361 gctgccggac cacccgtggt tcgtggcggg acagttccac cccgagttca agagccgtcc
     3421 ggaccgcccg cacccgctgt tccgcgactt cgtgggggcc gcgctggcgc attccggcgc
     3481 cgacgcggca gtcgacctca ccgagacccg catctagacg cactgccctc ggcgggcggg
     3541 ccggcttcgg ctacccgcag gtccggcgtt cgatgtactc cgtggcgggg tccgcggagt
     3601 ggtacccgac cgcccagccg gcgccgtccg gcgcgaggtc cacggccagg ggccagttgt
     3661 tctgagtctc gtcgtcctgg ccggcccggc tccacgaggt cccgtcccac tcgatcacga
     3721 cgcggctgtc gtgatcgtcg tgctggtcct cgcccaccgc gacggccgtg tcggcgtcga
     3781 gggccgagac tccgcggagg gtcacgaagg tcccgaggtc cgggagctcg gccgccgacc
     3841 agctcgtgcc gtcgaagtgg atggcccaag cccgcgggtc tccgcggtcg taccactcgc
     3901 cgaccgccca gacgtcgtcg ggggccgaac ccgagacgcc atagaacacg ctgaacccgt
     3961 cgcctggaga cggggtcggg acctcggtcc agcccgtccc gtcgaagtgg accgcgaacg
     4021 cgcgtcctct cccgtcgacg gatttcgtgc ccaccgccca gacgtcgtcc tcggcgacga
     4081 cgtcgacgtc ccagaggacg ccggggtagc ggccggcgac catcttccag gccgtgccgt
     4141 cgtagtgatg gaccatcgtg cggaaccgcc gcttcgcgtt gaatcgagtg cccgctgcgt
     4201 agacgtcacc ggacggcgcc acgtccaccc cgtagaggac gttccggtcc gcgctggggt
     4261 tcaggctggg ccgcatccgc cacgacgtcc cgtcgtacct ctgcacgatc gtcttcacct
     4321 tggtctgtcc gcgttcgccg acggcccacg cgg
//
LOCUS       DASQDO010000088         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_989022, whole genome shotgun sequence.
ACCESSION   DASQDO010000088 DASQDO010000000
VERSION     DASQDO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989022"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VEV43_02675"
     CDS             <1..512
                     /locus_tag="VEV43_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S15"
                     /protein_id="HYP22454.1"
                     /translation="VTRGARAYNLASNSGTCLRLDALPAEQSFDVDPTGLGAWAAPSG
                     PVAPHHVMLTEGPLTVAGEPRLSGQLLGLGVDARAFVALSIGSAESMCADARVVQNNV
                     MPLRSFAPDAGPRSFDVALPAVAATLRSGQALCLTISPQSDQSFGHGGKAPGAIVLQD
                     VQVRVPLSR"
     gene            540..1151
                     /locus_tag="VEV43_02680"
     CDS             540..1151
                     /locus_tag="VEV43_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22455.1"
                     /translation="MRIVLEGAIAGLGFSSGDCFVVGLWDSGPLGPMADVMWAQPSGR
                     RVLLAPDERVAAFVAGVYSFDETRVVPFDVTRSADGFELSAGELRVGAALGRRHPLFA
                     LRPRRLRRSLAWVRVEDAVLRPLVGRFLIGGGGGVRMRGTTPGGRREWYRIDGYRKVL
                     SATGRLGGRDLGPLTSLRPDLGFGFSGFPTRPAFVTCSPVLEG"
     gene            complement(1148..2278)
                     /locus_tag="VEV43_02685"
     CDS             complement(1148..2278)
                     /locus_tag="VEV43_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22456.1"
                     /translation="MRKLALAATALLISAVAPAATAVRGGPPLGPHFSSPNVEYLGHV
                     PLENDTAGARVVGRYLFVTTSRALTIYDLKDPLGPVKAGWTPMPQSPYFPQEDVDTNG
                     RILLVPSIDGVLNVVDVEDKTNPRVIGRLDGGASHTNTCVLDCTYSYASGGQIVDLRD
                     PANPKLAGDWKKTAGLPPSSTHDVTEVAPGMIVTSGPIALLDARKDPTKPVVRAFAWD
                     DGPGGIVHSNLWPRGMRDRYLLVGGESAGPRCTDAKASFTTYDTRGWRDSKTFRKLDQ
                     YRPPHGTYTDGNPAANTFCMHWFDDHPEFRDGGLVAVAWYEHGTRFVEVGRGGRIREA
                     GHFLPVGASTSAAYWVTDEIVYTADYQRGIDILRFDLGAPSG"
     gene            complement(2372..2665)
                     /locus_tag="VEV43_02690"
     CDS             complement(2372..2665)
                     /locus_tag="VEV43_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22457.1"
                     /translation="MRKITTVAAAAAALMALAVPTASAQPVPPVGDTSGCTTVLLDES
                     QTWVPTHMPNNWVEVTYTPPTTVTVSADRGYNYLSGIAFNITGDVVAFVDCVV"
BASE COUNT          329 a          926 c         1013 g          447 t
ORIGIN      
        1 gggtgacgcg gggcgcgcgg gcctacaacc tcgcgagcaa ctccggaacc tgcctgcggc
       61 tcgacgcgct cccggccgag caaagcttcg acgtcgatcc cacgggcctc ggcgcctggg
      121 ccgcgccgag cggtcccgtc gccccccacc acgtgatgct gaccgaaggc ccgctgacgg
      181 tcgcggggga gcccaggctg agtgggcagc tcctcggcct cggggtcgac gcgcgcgcgt
      241 tcgtggcgct gtcgatcggg tcggccgagt ccatgtgcgc tgacgcccgc gtcgtgcaga
      301 acaacgttat gcccctgcgt tcgttcgcgc cggacgcggg accccggagc ttcgacgtcg
      361 cgctgccggc ggtggccgcg acgctgaggt ccggacaggc gctgtgcctg acgatctcgc
      421 cgcaatcgga ccagtcgttc gggcacgggg gcaaggcgcc gggcgcgatc gtgctgcagg
      481 acgtacaggt gcgggtgccg ctgtcgaggt agccgccggg ccgtgggtag gtctcgggca
      541 tgcgcatcgt gctcgagggg gccatcgccg ggcttgggtt ctcgtccggc gactgcttcg
      601 tcgtcgggct gtgggactcg gggcctctgg gaccgatggc ggacgtcatg tgggcgcagc
      661 cgtcggggcg gcgggtgttg ctcgcgcccg acgagcgagt cgccgccttc gtagccgggg
      721 tctactcgtt cgacgagacg cgcgtcgtgc cgttcgacgt cactcgctcg gcggacgggt
      781 tcgagctgtc cgcaggcgag ctgcgcgtcg gcgcggccct cgggcggcgg cacccgctgt
      841 tcgcgttgcg tccgcggcgg ctccggcgtt cgctcgcgtg ggtgcgcgtc gaggacgcgg
      901 tcttgcgtcc gctcgtcggt cgcttcctga tcggcggggg cgggggagtc cgcatgcgcg
      961 gcacgacgcc gggcggccgg cgtgagtggt accgcatcga cggttaccgc aaggtcctgt
     1021 cggctaccgg gcggctcggg ggccgcgacc taggccccct cacgtccttg cgccccgacc
     1081 tgggattcgg cttcagcggg ttcccgaccc ggccggcgtt cgtgacgtgc tcgccggtgc
     1141 tggagggtta gccggaggga gcgccgaggt cgaacctcag tatgtcgatg ccccgctggt
     1201 agtcggccgt gtacacgatc tcgtccgtga cccagtacgc cgcgctcgtc gacgcgccca
     1261 caggcaggaa gtgaccggcc tcgcggatcc ggcccccgcg gcccacctcg acgaacctcg
     1321 tcccgtgctc gtaccacgcg actgcgacga gccctccgtc gcggaactct ggatggtcgt
     1381 cgaaccagtg catgcagaac gtgttcgcgg ctgggttccc gtcggtgtag gtcccgtgcg
     1441 ggggccggta ctggtcgagc ttgcggaacg tcttcgagtc gcgccagccc cgcgtgtcgt
     1501 acgtcgtgaa cgacgccttg gcgtccgtgc agcgggggcc ggcgctctcg ccgccgacga
     1561 ggagatagcg gtcccgcatc ccgcgcggcc acaggttgct gtggacgatg ccgcccggcc
     1621 cgtcgtccca cgcgaacgca cggaccacgg gcttcgtcgg gtccttccgc gcgtcgagca
     1681 gcgcgatcgg gcccgaggtg acgatcatcc ccggcgcgac ctcggtcacg tcgtgggtgc
     1741 tgctcggcgg gagccccgcg gtcttcttcc agtcgccggc gagcttcggg ttcgccgggt
     1801 cgcggaggtc cacgatctgg cccccggacg cgtacgagta cgtgcagtcg agcacgcacg
     1861 tgttcgtgtg gctggctcca ccatccaggc ggccgatcac ccgcgggttc gtcttgtcct
     1921 cgacgtcgac gacgttcagc acgccgtcga tcgacgggac cagcaggatc ctgccgttgg
     1981 tgtcgacgtc ctcctgcggg aagtacgggc tctgcggcat cggggtccag ccggccttca
     2041 ccgggcccag cggatccttc aggtcgtaga tcgtcagcgc ccgagacgtc gtcacgaaca
     2101 ggtagcgccc gacgacccgc gctccggcgg tgtcgttctc gagcgggacg tgcccgaggt
     2161 actcgacgtt gggggaagag aagtgggggc cgagcggggg cccgccgcgg accgccgtcg
     2221 ccgccggcgc gacggcggag atcagcagtg ccgtggccgc gagcgcgagc ttcctcatgc
     2281 ctcttgcttc tccggagcga gtgccggctc ctgtggcaag catgggcacc gagggggacc
     2341 cgtcaccgga tccccctcgg ctagggaccg tctagacgac gcagtcgacg aaggcgacga
     2401 cgtcgcccgt gatgttgaag gcgatgccgg agaggtagtt gtagccgcgg tcggcgctga
     2461 cggtcaccgt ggtgggcggc gtgtaggtca cctcgaccca gttgttcggc atgtgggtcg
     2521 gcacccaggt ctgcgactcg tccagcagga ccgtcgtgca tcccgaggtg tcacccacgg
     2581 gtgggacggg ctgtgccgac gcggtgggta ccgccagggc catgagcgcg gccgctgcgg
     2641 ccgcgaccgt cgtgatcttc ctcatctcgt cctcctcgga ttcgtgtggg tcagacgacg
     2701 cagtcgaccc aggtg
//
LOCUS       DASQDO010000089        10330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_254499, whole genome shotgun sequence.
ACCESSION   DASQDO010000089 DASQDO010000000
VERSION     DASQDO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254499"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1028)
                     /locus_tag="VEV43_02695"
     CDS             complement(177..1028)
                     /locus_tag="VEV43_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYP22458.1"
                     /translation="MPSPVSKAVLVTGSSSGIGRATALHLAERGWDVYASARRPESIA
                     DLGEAGCRIVQLDVTDEGSMVAAVRRIEAERGAVGVLVNNAGYGQGGAVEEVPVDAMR
                     AQFETNFFGLARLTQLVLPGMRQQRWGKVVNVSSMGGKLTLPGGGVYHATKHAVEALS
                     DALRFEVKGFGVDVIVIEPGVIKTGFGDVNVGSIDLGDPDGPYSYFNAGVARVVAGAY
                     SPKARGMGTSEGVAAVIERAIAARRPKTRYVYPAMGRALITARRLLPDRAFDVLLRSR
                     YPAPRPR"
     gene            complement(1276..1641)
                     /locus_tag="VEV43_02700"
     CDS             complement(1276..1641)
                     /locus_tag="VEV43_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22459.1"
                     /translation="MTALDEKPQKLCPKCGRVMWIVDVEGMGRLWQCASCKASMAGGT
                     LYMWREQEAAATAKVEVRTSPTKKYVSNKPCPECAEPMIAVQVPDYGTRHQCESCRLS
                     VMFGGSVVRWKSRDRASEG"
     gene            complement(1638..2708)
                     /locus_tag="VEV43_02705"
     CDS             complement(1638..2708)
                     /locus_tag="VEV43_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYP22460.1"
                     /translation="MNPEGVARPPRSSSPAGERRGLVLGGGGLVGMGYHAGALQALEE
                     WGVDLGASDLIVGTSAGAVMASYIASGWTGNDFYEYAHGRHPQSERDDAEQRDQVKTI
                     FTPMWHSPAERVRRGIGSTFAVVSSRGYWGKAMRGRVPSGTLRKVFPAGLYSTEDTRF
                     RLHADLPEEWPRDGVYICAADLYTGERVAFGAPGAPPAPFPEAVRASTSIPGVFPPVR
                     IGDRQYVDGGVVSATSLDLAADAGCGSILCVAPLGFRKEEAARTADPRLWSSMLVRSL
                     FARSLRREVLAARSAGVDVFVIRPWLTDLKAHGTNSMRHFDRAALTRSAREGTLRLLE
                     QNADHPALVAATTKRRAESAGG"
     gene            complement(2718..3629)
                     /locus_tag="VEV43_02710"
     CDS             complement(2718..3629)
                     /locus_tag="VEV43_02710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22461.1"
                     /translation="MDFVLERPTDAHLDELVELYRACDLVDAPGTVSDREDVAWRWRT
                     SDFDRSRDAWVARRDGVVAAYAWVFEGLADVKVHPDARGHGLGRRLLETTERRAAEQG
                     SRDGNLRQNVTNRMPDARALLEANGYVYSHHYARMEIDLAEAPGVPPPPEGVVLRNYE
                     VGPDDAIVHAAFNRAWSQYEGERWEPEPLDRWMDAVESEDFDAAHWHLAIEDGDVVGF
                     CLGERYGDNGWIQYLGIVPEQRGRGLGRVMLMRGLAGFHAEGVRRVELTVSSENVPAA
                     RALYESAGMRETLRYENLKKPMTAVGR"
     gene            3658..5583
                     /locus_tag="VEV43_02715"
     CDS             3658..5583
                     /locus_tag="VEV43_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HYP22462.1"
                     /translation="MITITGLRKSFGARDLFRDATVQVGARDRIAIVGLNGTGKTTLF
                     RIVAGEQEADEGDVVIARGVVVGYLRQETDALRGRTLLEEVLSAGSEATETGHRLAVL
                     EDEMQGLSPGPELEALVAEYARLQDRFATLGGYEIEPEAKRILSGLGFAQEAFDEPTD
                     TLSGGWLMRVALAKLLLSAPDVLLLDEPTNHLDLESVDWLERFLRGYEGAVLLISHDR
                     DFINGIATKVVEIDRARLTTYTGNYEAFVRQRAERARLAEAAAVNQQRKTAQTQAFID
                     RFRYKASKAKQVQSRVKALEKMEKAEGPGRRQRTMKLAFPQPPRPGRVVLDLAGVGFS
                     YGEKRVYDSLDFVIERGQKIALVGPNGAGKSTLLKLLAGALQPQSGERKLGHEARVGY
                     FAQHQIEELVLSNTVVQELQRAVPPGVEVQPRDLLGRFLFSGEDVDKRVSVLSGGERS
                     RLALARMLVTPANLLCMDEPTNHLDIPSRDALEDALEEYTGALVLITHDRHLIRSVAD
                     RIVEVRDGAVTSYDGSYDYYLERAVSDAEPESPAAQKEAPRLSAKERRRIEAQARALT
                     KDLRDKVTKLERRIGEVGAELKKLEAIMADPEVYSSGTDVAALVRDYESAKRRMARLE
                     GEWETAAESLERAQAGA"
     gene            complement(5585..6025)
                     /locus_tag="VEV43_02720"
     CDS             complement(5585..6025)
                     /locus_tag="VEV43_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22463.1"
                     /translation="MPRISFTGEEHARINHVLRELDPATHDLTAHWRDLLSGEDWAFL
                     QDYVRTEMRATLEMGMLARSIAQVVDERRLADQTAVDHFLRSTPWSRNFGLTDEAEER
                     LMSILGQTLDSPLFLAIEALMQIAEDRMFDTLDSEYRSLLEGRL"
     gene            6216..6752
                     /locus_tag="VEV43_02725"
     CDS             6216..6752
                     /locus_tag="VEV43_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HYP22464.1"
                     /translation="MARSEKQTERDREVAALFYEHYDPMRRLAYVMIGDGDAAEEIVM
                     EAFAKALSRWKSFRTVDWPPAYLRQMVVNACRSKMRRKAIENRVNSFVHHRDEQREKT
                     FDVEDHGLGLDVWAAVRSLPDRQRACVVLRYLEGMTEPEIAETLDVPLGTVKSQLSRG
                     RARLADKLGPDFMRGGES"
     gene            6749..7855
                     /locus_tag="VEV43_02730"
     CDS             6749..7855
                     /locus_tag="VEV43_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22465.1"
                     /translation="MNDGEGRIEEALRAVGDDYVRRNPPDLLRARNRIDQLRRRRQMK
                     ATAGAAFATAAVVAVAVLAWPGAQTAREAPRPAAEIELPAGWVSVSVGDEPTEVAVGD
                     GALWVSNAGDGTVSRIDPVSNEVVTVPLSGAPGDLAVGEGGEVWAASPELGAVQRIDP
                     VTSSPTPDRRIDVAPADTPLDLAIDGFLWVSVIDRDLVQVDPATGDEVRRIDWVRPVN
                     VAAREGAVFVLDAGGTVYGVDRLTGEPTGFEISFDVEDRGDVHYDDGHVWVAEGDGSA
                     VHALDFAGASRRIRSYGFRGTYVEMVHTPAGVVVLSDLGDGSGVLTQIDAATGETTEL
                     GEIEGGPRDLVQGSGAYWVSTSDADAVVRIPSLP"
     gene            7938..8471
                     /locus_tag="VEV43_02735"
     CDS             7938..8471
                     /locus_tag="VEV43_02735"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYP22466.1"
                     /translation="MSKPGPIAVTVIGTDRPGIVAGVTRALYEAGCNLEDVTSTILRG
                     HFSMVMVVRGPEGADAARLEDALAPAAREMDLVVAARPVEEASIALEDSTHVVSVYGA
                     DHPGIVYRVADALAGLGANVTDLTSRLIGSPDEPVYALMLEISAPSPDEVAAALDELR
                     GDVGVEVSMHPIERDVL"
     gene            8474..8965
                     /locus_tag="VEV43_02740"
     CDS             8474..8965
                     /locus_tag="VEV43_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYP22467.1"
                     /translation="MTVRPVCRLPAPILKAKAEPGPPDEELAADLLDTMRSSPACVGL
                     AAPQIGVSRRAFALDVSEHPKTTVCHGLVVLFDPVIVESSGRKVGREGCMSVPDLTAD
                     VARATSLVVVGTTPEGERKVIATEGFEARAFQHEIDHLDGLLILDRVASLATDIFRRK
                     VYK"
     gene            complement(8993..9283)
                     /locus_tag="VEV43_02745"
     CDS             complement(8993..9283)
                     /locus_tag="VEV43_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22468.1"
                     /translation="MARLDVELIEHELLQHPAVERCVVVARRTTTTRDPRMVAYVTGR
                     DLEESVLKSYVESHVFGFVLPEAYVVLDQLPLGPNGKVDRNALPDPFQFDRE"
     gene            complement(9344..>10330)
                     /locus_tag="VEV43_02750"
     CDS             complement(9344..>10330)
                     /locus_tag="VEV43_02750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYP22469.1"
                     /translation="EALAGRASRLAPQGDPRGVAAELLESVCATFGFARGVVAGGQGG
                     AIELLAYHGPGEMPVVAPCTGGLVRRVVDERRPILSKALDAESDGWLSGILPFARNLV
                     AVPLAADGEPVGALVVEHTGGRVERRVVSMLGQFAAHAALALRNSWLLQQVQRLAETD
                     GLTGLANRRTFETTLERELSRAARSGEPVTLVMVDVDHFKKLNDTHGHQTGDEVLKLV
                     AGELASACRDFDTAARYGGEEFAVILPSCSSSESLVVGERLRESIAAIETVTDVTASA
                     GVATFPVHASDPEGLIKAADEALYESKRAGRNRVTRSRRRGPKKSKLVVLPD"
BASE COUNT         1546 a         3639 c         3666 g         1479 t
ORIGIN      
        1 gcttcgtcgg gctcgtcctc ggcgcgctgc tcggctggac cgtgttcggc gggtcgaccc
       61 aggcggtcgt catcagcgcg gtggcgttcg cgttcgccgg cggcaccgcg ggcatgctga
      121 ttggcggctt catggctccg cgaaagaagc tcccgaacgc cggcgccgac aagtagctag
      181 cgcgggcgcg gcgcggggta gcgcgagcgc agcagcacgt cgaacgcgcg gtcgggcagc
      241 agcctccggg ccgtgatcaa cgcccgtccc atcgcgggat acacgtagcg ggtcttcggt
      301 ctgcgagcgg cgatcgcgcg ctcgatgacc gcggcgacgc cttcgctcgt ccccatcccg
      361 cgcgctttcg gcgagtacgc gccggccacg acgcgcgcga cgcccgcgtt gaagtacgag
      421 tagggcccgt ccgggtcccc gaggtcgatc gagccgacgt tgacgtcgcc gaaacccgtc
      481 ttgatcacgc cgggctcgat cacgatcacg tctacgccga agcccttgac ctcgaagcgc
      541 agcgcgtcgc tcagcgcctc gaccgcgtgc ttggtcgcgt ggtagacgcc gccgccggga
      601 agagtgagct ttccgcccat cgagctcacg ttcacgacct tgccccatcg ctgctgccgc
      661 atccccggca gcacgagctg cgtgagccgt gccagcccga agaagttggt ctcgaactga
      721 gcgcgcatgg cgtcgaccgg cacctcttcg acggcgccac cctggccgta tcccgcgttg
      781 ttcacgagga cgccgacggc gccgcgctcg gcctcgatcc tccgcacggc cgcgaccatc
      841 gagccctcgt cggtgacgtc gagctgcacg atccggcagc cggcctcgcc gaggtcggcg
      901 atcgactccg gcctccgggc ggatgcgtag acgtcccacc cgcgctccgc gaggtgcagg
      961 gcggtcgcgc gcccgatccc ggacgacgag ccggtgacga ggactgcctt ggagacggga
     1021 gatggcatca ggccgccgag gatgccagag aacccggacg aagaagagat gccccctggc
     1081 aggtcgagcg gctgggccgc taccagttcg aggagacgag ccgggcgttc gacgcgctgt
     1141 ctgcccgctt cggcgttccg tccctctttt cggacttcgg agctagccga gagtcctgcc
     1201 ggggtgcatc tcttgccctg acgctatgcc cccctgccgg gacggtcgat acgtatcctg
     1261 cgaaggcgag cccggtcagc cctccgacgc gcggtcgcgc gacttccacc gcacgaccga
     1321 cccgccgaac atcacggaca accggcacga ctcgcactga tggcgcgtcc cgtagtcggg
     1381 cacctggacg gcgatcatcg gctcggcgca ctccgggcac ggcttgttcg acacgtactt
     1441 cttcgtgggc gacgtgcgga cctcgacctt cgccgtcgcc gcggcctcct gctcgcgcca
     1501 catgtagagc gtgccgccgg ccatgctcgc cttgcacgag gcgcactgcc acagccgccc
     1561 catcccctcg acgtcgacga tccacatgac gcgcccgcac ttcgggcaca gcttctgcgg
     1621 cttctcgtcg agtgcggtca tccgccggcg ctctcggcgc gccgcttcgt cgtggcggcg
     1681 acgagggccg ggtggtccgc gttctgctcg agcagccgca gcgtcccctc gcgcgccgac
     1741 cgggtgaggg ccgcgcggtc gaagtgacgc atcgagttcg tcccgtgtgc cttcaggtcg
     1801 gtcagccacg ggcggatcac gaagacgtcg actccggcgg agcgcgcggc cagcacctcg
     1861 cgccgcagcg accgtgcgaa cagcgaccgg acgagcatgc tcgaccacag ccgcgggtcc
     1921 gccgtccgcg ccgcctcctc cttgcggaag cccagcggag cgacgcacag gatcgagccg
     1981 cacccggcgt ccgccgcgag gtcgagcgac gtggcgctta cgacgccgcc gtcgacgtac
     2041 tggcggtccc cgatgcgcac cggagggaag acgccgggga tggaggtcga cgcgcgcacg
     2101 gcttcgggga agggcgcggg gggagccccg ggagccccga acgcgacccg ctcgccggtg
     2161 tacaggtcgg cggcgcagat gtagacgccg tcgcgcggcc attcctcggg caggtcggca
     2221 tgcagccgga agcgcgtgtc ctcggtcgag tagaggcccg cggggaacac cttgcggagc
     2281 gtgccggacg ggacccgccc gcgcatcgcc ttgccccagt agccccggga cgacacgacg
     2341 gcgaacgtcg acccgatgcc cctccggacg cgttcggccg gcgagtgcca catcggcgtg
     2401 aagatcgtct tgacctggtc gcgctgctcg gcgtcgtcgc gctcggactg gggatggcgc
     2461 ccgtgcgcgt actcgtagaa gtcgttgccg gtccagcccg acgcgatgta cgaggccatg
     2521 accgcgccgg cgctcgtccc gacgattagg tccgaggccc cgaggtcgac gccccattcc
     2581 tcgagcgcct gcagcgcgcc ggcgtgatag cccatcccga cgagaccgcc tccgccgagc
     2641 acgaggccgc gccgctcccc ggccggggag gagcttcggg ggggccgggc tacgccctcg
     2701 gggttcacag gcagaaacta ccgcccgacg gcggtcatcg gtttcttcag gttctcgtag
     2761 cgcagggtct cgcgcatgcc cgcgctctcg tagagggccc gcgcggccgg gacgttctcg
     2821 gagctcaccg tcagctcgac ccgccgaacg ccctcggcgt ggaagccggc gagccctcgc
     2881 atgagcatga cccggcccag cccccgtccg cgctgctccg gcacgatccc gaggtactgg
     2941 atccagccgt tgtcgccgta acgttcgccg aggcagaacc cgacgacgtc gccgtcttcg
     3001 atcgctaagt gccagtgggc cgcgtcgaag tcctccgact cgaccgcgtc catccaccgg
     3061 tccaggggct ccggctccca ccgttcaccc tcgtactgcg accacgcacg gttgaacgcc
     3121 gcgtggacga tcgcgtcgtc gggaccgacc tcgtagttgc gcagcacgac gccctcgggt
     3181 gggggaggga cgcccggcgc ctcggctagg tcgatctcca tgcgcgcgta gtggtgcgag
     3241 tagacgtacc cgttcgcctc gaggagcgcc cgtgcgtccg gcatccggtt cgtgacgttc
     3301 tgacggaggt tgccgtcgcg gctgccctgc tcggccgccc gcctctcggt cgtctcgagg
     3361 agccggcgcc cgaggccgtg cccgcgcgca tccgggtgga ccttgacgtc ggcgaggccc
     3421 tcgaagaccc acgcgtaagc ggccacgacg ccgtctcgcc gcgctaccca tgcgtcgcgg
     3481 ctccggtcga agtcggaggt ccgccagcgc cacgcgacgt cctcgcgatc gctcaccgtt
     3541 cccggcgcgt cgacgaggtc gcaggctcgg tacagctcga cgagctcgtc gaggtgggcg
     3601 tcggtcggac gctccagcac gaagtccatc gcgcgacggt acagaatcga cggagtcatg
     3661 atcacgatca ccgggctcag gaagtcgttc ggggcgcgcg acctgttccg cgacgcgacc
     3721 gtccaggtcg gcgcgcgcga ccgcatcgcg atcgtcgggc tgaacggcac gggcaagacg
     3781 acgctgttcc gcatcgtcgc cggcgagcag gaggccgacg agggcgacgt cgtgatcgcc
     3841 cggggcgtcg tagtgggcta cctgcgccag gagaccgacg ctctgcgggg ccggaccctt
     3901 ctcgaagagg tcctgtccgc cggctccgag gcgaccgaga ccgggcaccg gctcgcggtg
     3961 ctcgaagacg agatgcaagg cctctcgccc ggccccgagc tggaggcgct cgtcgccgag
     4021 tacgcacggc tccaggatcg cttcgcgacg ctcggcggct acgagatcga gcccgaggcg
     4081 aagcggatcc tgtcggggct cggcttcgcg caggaggcct tcgacgagcc gaccgacacg
     4141 ctgtcgggag gatggctgat gcgcgtggca ctggcgaagc tgctgctgtc ggcccccgac
     4201 gtgctcctgc tcgacgagcc cacgaaccac ctcgacctcg agtccgtcga ctggctcgaa
     4261 cggttcctgc gcggctacga gggagcggtg ctgctgatct cgcacgaccg cgacttcatc
     4321 aacgggattg ctacgaaggt cgtcgagatc gaccgcgccc ggctcaccac gtacacgggg
     4381 aactacgagg cgttcgtgcg gcagcgcgcc gaacgggcgc ggctcgccga ggccgcggcc
     4441 gtgaaccagc agcgcaagac cgcccagacg caggcgttca tcgaccgctt ccgctacaag
     4501 gcttcgaagg cgaagcaggt ccagagccgg gtcaaggccc tcgagaagat ggagaaggcg
     4561 gaggggccgg gccggcggca gcgcacgatg aagctggcgt tcccgcagcc gccgcggccg
     4621 ggccgcgtag tgctcgacct cgccggcgtc gggttctcgt acggagagaa acgcgtctac
     4681 gactcgctcg acttcgtgat cgagcggggg cagaagatcg cgctggtcgg cccgaacggc
     4741 gccggcaaga gcaccttgct caagctcctc gccggtgcgc tgcagccgca gtccggtgag
     4801 cgcaagctcg gccacgaggc gcgcgtcggc tacttcgccc agcaccagat cgaagagctc
     4861 gtgctctcga acaccgtcgt gcaggagcta cagcgcgcgg tcccaccggg tgtcgaggtt
     4921 cagccccgcg acctcctcgg ccgcttcctg ttctccggcg aggacgtcga caagcgcgtg
     4981 tcggtgctgt ccgggggcga acggagccgg ctcgccctgg cccggatgct cgtcaccccc
     5041 gccaacctcc tgtgcatgga cgagccgacg aaccacctcg acatcccgag ccgcgacgcc
     5101 ctcgaggacg cgctcgagga gtacacgggg gcgctcgtcc tcatcacgca cgaccgtcac
     5161 ctgatccgca gcgtcgccga ccgcatcgtc gaggtgcgcg acggcgccgt cacgtcctac
     5221 gacggcagct acgactacta cctcgagcgc gccgtctccg acgccgagcc ggagtcgccg
     5281 gcagcccaga aggaggcgcc ccggctgagc gcgaaggaac gccgccgtat cgaagcgcag
     5341 gcacgcgcgc tgacgaagga cctgcgcgac aaggtgacga agctcgagag acggatcggg
     5401 gaggtgggtg ccgagctgaa gaagctcgag gcgatcatgg ccgaccccga ggtctactcg
     5461 tcgggcaccg acgtggcggc gctcgtgcgc gactacgagt cggcgaagcg gcggatggcg
     5521 cgcctggagg gggagtggga gacggcggcc gagagcctcg aacgggccca ggccggtgct
     5581 tgagctagag ccggccctcg agcaggctcc ggtactccga gtcgagcgtg tcgaacatcc
     5641 ggtcctcggc gatctgcatg agcgcctcga tcgccaggaa caggggggag tcgagggtct
     5701 ggcccaggat gctcatcagg cgctcctcgg cctcgtcggt cagcccgaag ttccgggacc
     5761 agggcgtcga gcgcaggaag tggtcgacgg ccgtctgatc ggcgaggcgg cgctcgtcca
     5821 cgacctgggc gatcgagcgg gcgagcatgc ccatctcgag cgttgcccgc atctcggtgc
     5881 gcacgtagtc ctgtaggaac gcccagtcct caccgctgag gaggtcgcgc cagtgagccg
     5941 tgaggtcgtg ggtcgccggg tcgagctcgc gcaggacgtg gttgatgcgt gcatgctctt
     6001 cgccggtgaa cgagattcga ggcacggggg gtccttggtc acggtggcgg tgcgaacgag
     6061 gtgcttcggc attatcggaa ccccgggact agtcccgcaa gagctttgcg aaaactacgt
     6121 acggatcggc ttccgggaac cgtccggccc ccttgggcgt gttgacgggc atggaagcgc
     6181 acctcgtgca cgcaggcgtc ctggtggagc gggacttggc taggtcggag aagcagaccg
     6241 agcgggaccg cgaggtcgcc gctctgttct acgagcacta cgacccgatg cgccggctcg
     6301 cctacgtgat gatcggcgac ggcgatgccg cggaggagat cgtgatggag gccttcgcga
     6361 aggcgctgtc gcggtggaag agcttccgca ccgtcgactg gccgcccgcg tacctccgcc
     6421 agatggtcgt caacgcctgc cgttcgaaga tgaggcgcaa ggcgatcgag aaccgcgtca
     6481 actcgttcgt ccaccaccgc gatgagcagc gtgagaagac cttcgacgtc gaagaccacg
     6541 ggctcggcct cgacgtgtgg gccgcggtgc gatcgctccc ggaccgccag cgcgcctgcg
     6601 tcgtcctccg ttacctcgag gggatgaccg agcccgagat agccgagacc ctcgacgtcc
     6661 cgctcgggac cgtgaagtca cagctctccc gcggcagggc gcgcctcgcc gacaagctcg
     6721 gcccggactt catgaggggc ggggagagtt gaacgacggt gagggccgga tcgaagaagc
     6781 gttgcgtgcg gtgggcgacg actacgtcag gcggaaccct ccggacctgc tccgggcgag
     6841 gaatcggatc gaccagctcc ggcgccggcg tcagatgaag gcgaccgccg gtgctgcgtt
     6901 cgcaaccgcc gcggtagtcg ccgtggcggt gctcgcatgg ccgggagcgc agacggcgcg
     6961 ggaagcgccg cggccggcgg ccgagatcga gctccccgcg ggctgggtct cggtctccgt
     7021 aggcgacgag cccacggagg tcgccgtcgg cgacggcgcg ctctgggtga gcaacgccgg
     7081 cgacggcacc gtgtcgagga tcgacccggt ctccaacgag gtcgtgacgg tcccgctgtc
     7141 gggcgcgccc ggcgacctgg ccgtcggcga gggcggcgag gtgtgggccg cgagcccgga
     7201 gctcggcgcc gtccagagga tcgacccggt gacgagctcc ccgactccgg accgcaggat
     7261 cgacgtggcg cccgcggaca cgccgctcga tctcgcgatc gacggcttcc tctgggtgtc
     7321 ggtcatcgac cgcgacctcg tgcaggtcga ccccgctacg ggtgacgagg tccggcggat
     7381 cgactgggtg cgcccggtga acgtcgcggc gcgagaggga gcggtcttcg tcctggacgc
     7441 cggcgggacg gtgtacggag tggatcgcct cacgggagag ccgaccggct tcgagatctc
     7501 gttcgacgta gaggaccgcg gcgacgtcca ctacgacgac gggcacgtgt gggtggccga
     7561 gggcgacggc agtgccgtgc acgccctcga cttcgccggg gcgtcgagga ggatccggtc
     7621 gtacgggttc cgcggcacgt acgtcgagat ggtgcacacg ccggcgggcg tcgtcgtgct
     7681 ctcggacctc ggtgacggca gcggcgtcct cacgcagatc gacgcggcga cgggcgagac
     7741 gacggagctg ggcgagatcg aaggggggcc gcgcgacctc gtgcagggga gcggcgccta
     7801 ctgggtctcg acgagcgacg ccgacgcggt cgtacgcatc ccctcgctgc cctagcccgg
     7861 cccgtctgcc atccaatgag gcgaaatgga cgcatagcgt ccgtttttcc tcagaggtcg
     7921 cgctttaggg tgggccgatg agcaagcccg gccctatcgc cgtcaccgtc atcggcaccg
     7981 accgccccgg catcgtcgcg ggcgtgacgc gggcgctcta cgaagcgggg tgcaacctcg
     8041 aagacgtgac ctcgacgatc ctgcgcgggc atttctcgat ggtgatggtg gtacgggggc
     8101 cggagggggc cgacgccgcc cggctcgaag acgcgctggc gcctgcggca cgcgagatgg
     8161 acctcgtcgt cgcggcgagg ccggtggagg aagcgagcat cgcgctcgag gactcgacgc
     8221 acgtcgtctc ggtctacggc gccgaccacc ccggcatcgt ctaccgcgta gcggacgcgc
     8281 tcgcggggct cggtgcgaac gtcaccgacc tgacctctcg tctcatcggc tcgcccgacg
     8341 agcccgtgta cgcgctgatg ctcgagatct ccgcgccgtc gccggacgag gtggccgccg
     8401 cgctcgacga gctccgagga gacgtcggcg tcgaggtcag catgcatccg atcgagcggg
     8461 acgtcctcta ggcgtgacgg tccggccggt ctgccggctg cccgccccga tcctcaaggc
     8521 gaaggccgag ccgggccccc ccgacgagga gctggctgcg gacctcctcg acacgatgcg
     8581 gtcgtccccc gcgtgcgtgg gcctggccgc gccgcagatc ggcgtctccc ggcgcgcgtt
     8641 cgcgctcgac gtcagcgagc accccaagac gaccgtctgc catgggctgg tcgtcctctt
     8701 cgacccggtg atagtcgaga gctccggccg caaggtgggc cgcgagggtt gcatgagcgt
     8761 gcccgacctg accgcggacg tagcgcgcgc gacgagtctc gtggtcgtgg ggacgacgcc
     8821 ggaaggcgag cgcaaggtca tagcgacgga gggcttcgag gcccgcgcct tccagcacga
     8881 aatagaccac ctggacggtc tgctgatcct ggaccgcgta gcttcgctgg cgaccgacat
     8941 cttccggagg aaggtctaca agtagtcccc ccggagctca gacagctcgc ggctactcgc
     9001 gatcgaactg gaagggatcg gggagcgcgt tgcggtccac cttcccgttg ggccccaagg
     9061 gcagctggtc gagcaccacg tacgcttccg ggagcacaaa gccgaagacg tggctctcga
     9121 cgtaggactt cagcaccgac tcctccaggt cgcggcccgt gacgtacgcg accatgcggg
     9181 ggtcgcgcgt agtcgtcgtc ctccgcgcga cgactacgca gcgctcgacg gctgggtgct
     9241 gcaacagctc gtgttcgatg agctccacgt cgaggcgtgc cacgtgagcg acattggcac
     9301 agggcgccct ccctgtcgac cgggaagtcg cggcgccgag cgctcagtcg ggcaggacga
     9361 cgagcttcga cttcttcggc ccccggcggc gcgagcgcgt cacgcggttc cggcccgcac
     9421 gcttcgactc gtacagcgcc tcgtccgctg ccttgatcag gccctcgggg tccgacgcgt
     9481 gcacggggaa ggtcgcgacg ccggcgctcg ccgtcacgtc ggtgaccgtc tcgatcgcgg
     9541 cgatcgactc gcgcagccgc tctccgacga cgagcgactc cgacgacgag cacgacggca
     9601 ggatcacggc gaattcctcg ccgccgtacc gggccgcggt gtcgaagtcg cggcacgcgg
     9661 acgcgagctc tcccgccacg agcttcagca cctcgtcgcc cgtctggtgg ccgtgcgtgt
     9721 cgttgagctt cttgaagtgg tccacgtcca ccatcacgag cgtcaccggc tcgccgctgc
     9781 gcgcggccct cgacagctcg cgctccagcg tcgtctcgaa agtccgccgg ttcgcaagcc
     9841 cggtgagccc gtccgtctcg gccaggcgtt ggacctgctg caacagccac gagttccgca
     9901 gcgcgagggc agcgtgcgcg gcgaactggc cgagcatcga gacgactcgc cgctcgaccc
     9961 ggcccccggt gtgctcgacg acgagcgctc cgacgggctc gccgtccgcc gccagcggaa
    10021 ccgcgacgag gttgcgcgcg aaggggagga tcccgctgag ccatccgtcc gactcggcgt
    10081 cgagagcctt cgacaggatc ggacgccgct cgtcgaccac gcggcgaacg aggccccccg
    10141 tgcacggcgc gaccaccggc atctcaccgg ggccgtggta ggcgagcagc tcgatggcac
    10201 cgccctgccc ccctgcgacc acgccgcggg cgaagccgaa cgtcgcgcac acggactcga
    10261 gcagctcggc cgctaccccc cgggggtcgc cttgcggcgc gagccgcgac gccctccccg
    10321 cgagtgcctc
//
LOCUS       DASQDO010000090         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_438041, whole genome shotgun sequence.
ACCESSION   DASQDO010000090 DASQDO010000000
VERSION     DASQDO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438041"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VEV43_02755"
     CDS             complement(<1..845)
                     /locus_tag="VEV43_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22470.1"
                     /translation="MDTDLFQILALILLLGLLLTLFGVLQSLGAIRKTLEGQGAPEPS
                     ATPAVEPVAAAPPREASQEPETWARGDATEPETSGLGDTGARTGWPAPQEAAGAPTST
                     VEETSPAPAAGSGARVLYGDAGLTTATPSEAALRPAEDEPADTTAAASAGAAGSGMAG
                     TMGLDSGVGTSGTTGGGTDAGTVGGLETGLGGSHMGTSGTSGTAGTDAGSGFGTVETG
                     GATSDTGGGTTGVGAATTDTSAGTGGGGAVTERAASAEDDPQEQPFERDGRWWFRRGD
                     ELLVY"
     gene            complement(885..1331)
                     /locus_tag="VEV43_02760"
     CDS             complement(885..1331)
                     /locus_tag="VEV43_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22471.1"
                     /translation="MQRARGHLVVVVGGVALLLTSFVPLWATYRVPGLGLVAPETSHQ
                     NAWTAYGLTMQLGLAVALVASLLAGISALTGSTERTPVVLGLCATAFALLIWQVVRGP
                     QGSSDPGGYGIDRGVLLFTGAALAAAMTYGSYFAWKETGRAPRVRG"
     gene            complement(1354..1545)
                     /locus_tag="VEV43_02765"
     CDS             complement(1354..1545)
                     /locus_tag="VEV43_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22472.1"
                     /translation="MPKLRSIAVATALAAAASLPLAPAAYASHSCALDETNPTLDQIC
                     ESHGDGIVQAILCLILKSC"
     gene            complement(1643..2800)
                     /locus_tag="VEV43_02770"
     CDS             complement(1643..2800)
                     /locus_tag="VEV43_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22473.1"
                     /translation="MNDFERRLRDLGEKARDEVSTGLAPRPGHLRRIRRRRLVASTGA
                     FACAATIVAAGAFAWAGRTADSPPVPPADDRPSPFVDTRACEPTSVAARVAPAGFSKT
                     LEMPRVTTGRGVLGVFRGPRDSAIEVTLAHYAYPQRRPRPIEMLGGRASIGAIRDGYS
                     VVLDEPCGIALNGYGITEAELERFARGLRRTNRMESLYGGTIWPPDDLTAEAQACTVD
                     MHERDDPVVVAKQFLATELGWEDAIVIRPKRWWEPWYATRSAPDAYETPPGVELRMGE
                     AIAPGCWSVESAGRYEGGVAPEGLAVSVRGERMTVSYAPEEGMLQGIADVRYGTRGPI
                     RHSVGSDGEGLVELGFEPKEPGRLLLHYQDQDKGVFYCIGIALPPGDYEFE"
     gene            complement(2797..3321)
                     /locus_tag="VEV43_02775"
     CDS             complement(2797..3321)
                     /locus_tag="VEV43_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HYP22474.1"
                     /translation="MATTGASMETAVRTTAAGRFEDLYAAHGRDAVRLAYLLTGDRSE
                     AEDVAQEAFVRLLGKFADLRKPEVFRAYLMRTVTNLVRSRFRHLAVERKHAGTVVRRG
                     DPVQMPDLGARAELGAALARLPYRQRAALVLRYCEDLSEQQTADVLGTSVKAVKGLVV
                     RGLAHLREQEEVSR"
     gene            3413..3616
                     /locus_tag="VEV43_02780"
     CDS             3413..3616
                     /locus_tag="VEV43_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HYP22475.1"
                     /translation="MAVIKTIDLVGVSTESWRDAANQALAEASKTIRGITGMEVLDTS
                     CNVSDGTISEYLTHVRIKFRIER"
     gene            3621..4895
                     /locus_tag="VEV43_02785"
     CDS             3621..4895
                     /locus_tag="VEV43_02785"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HYP22476.1"
                     /translation="MSGIALVGIQWGDEGKGKVTDLLAERTDVVVRYQGGNNAGHTIV
                     VGGERYALHLVPTGILYPHCTPVIGPGVVVNPQVLIEEMDNLEGRGIDTSRLRLSGNA
                     HVIMPYHLELDTVTERRLGKNRLGTTKRGIGPTYADKAARIGLRVQDLLDPKIFSEKL
                     KVALKEKNLVLTRVYGRLPLEQQKIEEEYLEYARRLEPHIGDTSLFVQRALDEGRSVM
                     FEGAQATMLDLDHGTYPFVTSSNPIAGGACAGAGVGPRDVQRIIGIAKAYCTRVGSGP
                     FPSEADPADAEVLVEAGGEYGTTTGRKRRCGWFDAVAARYAARLNTLTELVITKLDVL
                     SQFDSIKVCVAYEYEGERYEEFPPNQTIFNKCRPIFEELPGWGDDLTEARALDDLPKE
                     ARSYVEALESLARVPVSYVSVGPGRDQMVPLQ"
     gene            4892..>5036
                     /locus_tag="VEV43_02790"
     CDS             4892..>5036
                     /locus_tag="VEV43_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HYP22477.1"
                     /translation="MKVLLLGSGGRESALAWALSRSHVVDELVAAPGNPGIARHAALE
                     NVDS"
BASE COUNT          737 a         1819 c         1759 g          721 t
ORIGIN      
        1 tcgtagacga gcagctcgtc gccccgccgg aaccaccagc ggccgtcgcg ctcgaagggc
       61 tgctcctgag gatcgtcctc ggccgaggcc gcccgttcgg tgaccgcgcc gcccccgccg
      121 gtgcccgcgg aggtgtcggt cgtcgcggcc cccacgccgg tcgtcccgcc gcccgtgtcc
      181 gaggtcgccc cgccggtctc gacggtgccg aacccggacc ccgcgtccgt gccggcggtg
      241 ccgctcgtgc cgctcgtgcc catgtgggac ccgccgaggc cggtctcgag gccgccgacc
      301 gtcccggcgt ccgtgccccc accggtcgtg cccgacgttc ccacgccgga gtcgagcccc
      361 atcgtgccgg ccatcccgga ccccgccgcc cctgccgaag cggccgccgt cgtgtccgcg
      421 ggctcgtcct cggccgggcg cagcgcggcc tcgctcggcg tcgccgtcgt cagcccggcg
      481 tccccgtaca gcacccgtgc gccgctgccg gccgcaggcg cgggagaggt ttcctccacg
      541 gtcgaggtcg gggccccggc cgcttcctga ggcgccggcc agcccgtccg ggcgccggtg
      601 tcgccaagcc cggacgtctc cggctccgtg gcgtcgcccc gggcccacgt ctcgggctcc
      661 tggctcgctt cgcgtggggg ggccgcggcc accggctcga cggccggcgt agccgagggc
      721 tcgggggcac cttggccctc gagcgtctta cggatcgcgc ctagcgactg gagcacgccg
      781 aacagggtca gcagcaagcc cagcaacagg atcaacgcga ggatctggaa caggtccgtg
      841 tccatctcgg atcactctcc tagtgacggt gcgctttgcg cacatcatcc ccgaacgcgg
      901 ggcgcccggc cggtttcctt ccaggcgaag tagctaccgt aggtcatggc cgcggcgaga
      961 gcggcccccg tgaacaacag cactccgcgg tcgatgccgt atccgcccgg gtccgacgat
     1021 ccctgcgggc cccgtacgac ctgccagatc agcagcgcga acgccgtcgc gcacagcccc
     1081 agcacgacgg gggtccgctc ggtcgatccc gtgagcgcgg agattccggc gagcagcgag
     1141 gccacgagcg cgacggcgag ccccagctgc atcgtgaggc cgtacgcggt ccacgcgttc
     1201 tgatgcgagg tctcgggcgc gacgaggccg aggccgggga cgcggtaggt cgcccacaac
     1261 gggacgaacg acgtcaggag cagcgcgacg ccgcctacga cgacgacgag gtgaccccgc
     1321 gcgcgctgca cgcgcgcaat atagcgaggg tccctagcag gatttgagga tcaggcagag
     1381 gatcgcctgg acgatcccgt caccgtggct ctcgcagatc tggtcgagcg tcgggttcgt
     1441 ctcgtcgagg gcgcacgagt gcgaggcgta ggcggcgggc gcgagcggga ggctggcggc
     1501 cgcggccagg gccgtggcga ctgcgatcga acggagcttc ggcatcggag gtctccttcg
     1561 tcgggaggga ttgaccggtt tgcctggttt ggtatatcac cctggccgcc tgcggccacc
     1621 tagccgagcg ggcgcgtctg agctactcga actcgtagtc ccccggcggc agcgcgatcc
     1681 ctatgcagta gaagacgccc ttgtcctggt cctggtagtg gagcaggagc cggcccggct
     1741 ccttcggctc gaagccgagc tccacgagtc cctcgccgtc cgagcccacg ctgtgccgga
     1801 tcggcccccg cgtgccgtag cggacgtctg cgatgccctg gagcatccct tcctccggcg
     1861 cgtacgagac cgtcatacgt tcgcctcgga ccgagacggc taggccctcc ggggcgacgc
     1921 cgccctcgta tcgcccggcg ctctcgaccg accagcagcc cggagcgatt gcctcgccca
     1981 tcctcagctc gacccccgga ggcgtctcgt acgcgtccgg tgcggagcgc gtcgcgtacc
     2041 agggctccca ccaccgcttc ggtcggatca cgatcgcgtc ctcccagccg agctcggtcg
     2101 cgaggaactg cttggccacg acgacggggt cgtcgcgctc gtgcatgtcg accgtgcatg
     2161 cctgcgcctc ggccgtgagg tcgtccggcg gccagatcgt ccccccgtac aaagattcca
     2221 tccggttcgt acggcggagg ccacgggcga accgctcgag ctcggcctcc gtgatcccgt
     2281 agccgttgag agcgatgcca cagggctcgt cgaggacgac cgagtagccg tcgcggatcg
     2341 caccgatgga ggcccggccc ccgagcatct cgatcggccg cggtcgcctc tgcgggtacg
     2401 cgtagtgcgc aagcgtgacc tcgatcgcgg agtcacgtgg tcctcggaag acgccgagca
     2461 cgccgcggcc ggtcgtgacc ctgggcatct ccagcgtctt cgagaagccg gccggagcga
     2521 cccgcgcggc taccgacgtg ggctcgcagg ccctcgtgtc gacgaacggc gacggcctgt
     2581 cgtcggccgg cgggaccggc ggagagtcgg ccgtccggcc ggcccacgcg aacgctcccg
     2641 ccgccacgat cgtcgccgcg cacgcgaagg ctccggtcga ggccacgaga cggcgccgtc
     2701 tgatccgtcg caggtgcccc ggccgcggcg ccaggcccgt gctcacctcg tctctcgcct
     2761 tctcgccgag gtccctcagc cgtcgctcga agtcgttcat cgcgacacct cttcctgctc
     2821 tctcaagtgg gcgagcccgc gcactaccag gcccttcacc gccttcaccg aagtcccgag
     2881 cacgtcggcc gtctgctgct ccgacaggtc ctcgcagtaa cggaggacga gcgcggcccg
     2941 ctggcggtag gggagccggg cgagcgcggc cccgagctcg gcgcgggcgc cgaggtcggg
     3001 catctgcacg gggtcgccgc ggcgcacgac ggtgccggcg tgcttgcgct cgacggccag
     3061 atgacggaag cgactccgga cgaggttcgt gaccgtccgc atcaggtagg cccggaagac
     3121 ctcgggcttg cggaggtcgg cgaacttgcc gagcaggcgc acgaacgcct cctgagcgac
     3181 gtcctcggcc tcggagcggt cgccggtcag cagataggcg agccggacgg cgtcgcggcc
     3241 gtgcgcggcg tagaggtcct cgaacctccc cgccgccgtc gtccgcaccg ccgtctccat
     3301 gctcgctcca gtcgtggcca tgggttctac gtcaatagac gcccggaacc gccgggaggc
     3361 cccgcgtcgc tacaatccac cccgcggaga ccgactagcc agagaggtgc ccatggcggt
     3421 gatcaagacg atcgacctgg taggggtgtc cactgaatcg tggagggacg ccgccaacca
     3481 ggccctcgcg gaggcgtcca agaccatccg cggcatcacc ggcatggagg tcctcgacac
     3541 ctcgtgcaac gtctcggacg ggaccatcag cgagtacctg acgcacgtcc ggatcaagtt
     3601 ccggatcgag cgctgacgcg atgtccggca tagccctcgt cggcatccaa tggggtgacg
     3661 agggcaaggg aaaggtcacc gacctcctcg ccgagcgcac cgacgtcgtc gtccgctacc
     3721 agggcggcaa caacgccggc cacacgatcg tcgtcggggg cgagcgttac gcgctccatc
     3781 tggtcccgac ggggatcctc tatccgcact gcacgccggt gatcggcccc ggcgtcgtcg
     3841 tgaacccaca ggtcctgatc gaggagatgg acaacctgga gggccgcggg atcgacacgt
     3901 cgcggctcag gctctcgggg aacgcgcacg tgatcatgcc gtaccacctc gagctcgaca
     3961 cggtcaccga gcgcaggctc ggcaagaacc gcctcggcac caccaagcgc ggcatcggcc
     4021 cgacgtacgc ggacaaggcc gcgcgcatcg ggctgcgcgt gcaggacctc ctcgacccga
     4081 agatcttctc ggagaagctg aaggtcgccc tgaaggagaa gaacctcgtg ctgacgcgcg
     4141 tctacggtcg gctcccgctc gagcagcaga agatcgaaga ggagtacctc gagtacgcgc
     4201 ggcgtctcga gccgcacatc ggcgacacga gcctgttcgt gcagcgcgct ctcgacgagg
     4261 gacgctcggt gatgttcgag ggcgcgcagg ccacgatgct cgacctcgac cacggcacct
     4321 atccgttcgt gacgtcgtcg aacccgatcg cgggcggagc atgtgcgggc gcgggcgtcg
     4381 gcccccgcga cgtccagcgg atcatcggga tcgcgaaggc ctactgcacg cgcgtcggct
     4441 cggggccctt cccgtcggag gccgaccctg cggacgcgga ggtcctcgtc gaggccggtg
     4501 gtgagtacgg gacgacgacc gggcgcaagc gccgctgtgg atggttcgac gcagtcgcgg
     4561 cgcgctacgc ggcgcggctc aacacgttga cggagctcgt gatcacgaag ctcgacgtgc
     4621 tctcgcagtt cgactcgatc aaggtgtgcg tcgcctacga gtacgagggc gagcgctacg
     4681 aggagttccc cccgaaccag acgatcttca acaagtgccg gccgatcttc gaagagctgc
     4741 cgggctgggg cgacgacctg accgaggcgc gtgcgctcga cgacctcccg aaggaagcga
     4801 ggtcgtacgt cgaggcgctc gagtcgctgg ctcgcgtccc ggtgtcgtac gtgtcggtgg
     4861 gaccgggccg cgaccagatg gtgccgctgc aatgaaggtc ttgctgctgg ggtccggggg
     4921 tcgcgagagc gcgctcgcgt gggcgctgtc gcgctcgcac gtcgtggacg agctcgtggc
     4981 cgcgccgggg aaccccggca tcgcgcgcca cgcggctctg gagaacgtcg actcgt
//
LOCUS       DASQDO010000091         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_560392, whole genome shotgun sequence.
ACCESSION   DASQDO010000091 DASQDO010000000
VERSION     DASQDO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560392"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..923)
                     /locus_tag="VEV43_02795"
     CDS             complement(174..923)
                     /locus_tag="VEV43_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22478.1"
                     /translation="MMGLFGSEMTRLASRRLFRWLLILFIGFLVLGAVIVFVNDNGTF
                     SRENVADGLIGLSFPLLMFSWLVGASAIGAEWGNRTVSALLTWEPRRTRVLVTKAVAA
                     AVSTALWVIALQIVWTAVMMPVASDGVGFTVGPPFSWGDYLAFAARILGVCVVASVLG
                     FSLATIGKNTGAALGGGMAYLLIVESLVRAYLPEWNDWLLSTNIVRVAERGADAAFDG
                     VTTEEAALRLVAYAVGLFLVALGVFRRREMA"
     gene            complement(920..1840)
                     /locus_tag="VEV43_02800"
     CDS             complement(920..1840)
                     /locus_tag="VEV43_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP22479.1"
                     /translation="MAVIEVRGLKKDYRRLRGGSTRAVDGLDLTIEPGGVFGLLGPNG
                     SGKTTTFRCILGLARPTEGECRLLGADSQRGLHTVVGRVGSIVEAPALFPTFSGKRNL
                     ELLAAIHDIPDARVGSVLERVGLAGREKHLVRTYSLGMRQRLAIAAALLKDPELLILD
                     EPANGLDPAGIREVREMVRSLAAEGRTVVVSSHILSEVQLMCDRVAILSRGRCVAVGD
                     VEELLRQAQGSGVLVRLDDPSRGAEVLRAAGLEATESERGLHIAVPPDQGSRVARVLA
                     EAGLYPNELRPDEVDLETVFLDLTEGPASS"
     gene            1948..2619
                     /locus_tag="VEV43_02805"
     CDS             1948..2619
                     /locus_tag="VEV43_02805"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYP22480.1"
                     /translation="MTGTTEIAARLDRSTADDRLLDHPFYRAWADGTLTTDDLRFYAA
                     QYFNQVEAFPGYLAVLADRLPDSEAKTSVLENLADERDGDHLGLWLRFAAAVGADSVS
                     VRETPAEDETLACIAAFEAAVRDRSPSFALGMIYGYESQTPDVCETKIKGLRDLYGVE
                     GPGLEYFELHGELDVEHAGELAAAVAELSSSEDDLRDAEEGAVAGARAIRGLLDGVAR
                     VRGIG"
BASE COUNT          439 a         1038 c          940 g          344 t
ORIGIN      
        1 gacgcccgtc atggagccga cgaaacccgc caccgctacg gcgaacgccg ccgcccttgt
       61 cgatccgcga agcctcacag aagacacctc tctgacgtag aagaccctct ccggaccttc
      121 ctaccggccg ggggcctgga aataaatacg taattccgct gggcggggcc gggtcacgcc
      181 atctcgcggc gccggaagac ccccagcgcc acgaggaaca gcccgaccgc gtacgccacc
      241 agccgaaggg cggcctcctc ggtcgtcacg ccgtcgaagg cggcgtcggc gccgcgctcc
      301 gcgacccgga cgatgttcgt gctcaggagc caatcgttcc actcgggcag gtaggcgcgc
      361 acgagcgact cgacgatcag gaggtaggcc atgcccccac cgagggcggc ccccgtgttc
      421 ttgccgatgg tcgccaggga gaacccgagc acggacgcga ccacgcacac gccgaggatc
      481 cgcgcggcga acgcgaggta atcgccccac gagaagggag ggccgaccgt gaacccgacg
      541 ccgtcgctcg cgaccggcat catcacggcc gtccacacga tctgcagcgc gatcacccac
      601 agggcggtcg agacggccgc cgcgaccgcc ttcgtcacga gcacccgcgt ccgccgcggc
      661 tcccacgtca gcagcgcgga gaccgtcctg ttcccccact ccgcgccgat cgcgctggcg
      721 ccgacgagcc acgagaacat cagcaggggg aaggagagcc ctatgagccc gtcggcgacg
      781 ttctcccgcg agaacgtccc gttgtcgttc acgaacacga tgaccgcgcc gagcacgagg
      841 aacccgatga acaggatcag gagccaccgg aacagcctcc gggaggcgag ccgcgtcatc
      901 tcggagccga agagacccat cacgacgacg ccggcccctc ggtgaggtcg aggaagaccg
      961 tctcgaggtc gacctcgtcc ggccgcagct cgttcgggta gaggccggcc tcggccagaa
     1021 cgcgcgcgac cctcgacccc tggtcgggcg ggaccgcgat gtgcagcccg cgctcggact
     1081 cggtggcctc gagccccgcc gcccgaagga cctcggcccc cctactggga tcgtcgaggc
     1141 gcacgaggac cccgctgccc tgggcctggc ggagcagctc ctcgacgtcg cccacggcga
     1201 cgcagcggcc ccgcgacagg atcgcgacgc ggtcgcacat cagctgcacc tcgctaagga
     1261 tgtggctcga gacgacgacc gtgcgtcctt cggcggcgag cgaccggacc atctcgcgga
     1321 cctccctgat cccggcgggg tcgagcccgt tcgccggttc gtcgagtatc agcagctccg
     1381 ggtccttcag cagcgccgcg gcgatcgcga gccgctggcg cattcccagc gagtacgtcc
     1441 ggacgaggtg cttctcgcgc cccgcgaggc cgaccctctc gaggaccgag ccgacgcggg
     1501 cgtcggggat gtcgtggatc gcagcgagga gctcgaggtt ccgcttcccg gagaacgtgg
     1561 gaaagagcgc cggtgcctcg acgatcgacc cgacccgccc cacgaccgtg tggaggccgc
     1621 gctgtgagtc ggcgccgagg aggcggcact ccccctccgt cggcctcgcc agcccgagga
     1681 tgcaccggaa cgtcgtcgtc ttccccgacc cgttcgggcc gaggagcccg aacacgcctc
     1741 caggctcgat cgtgaggtca aggccgtcga ccgcccgggt cgagccaccc cgcagccgcc
     1801 ggtagtcctt cttcagcccg cgcacttcga tcaccgccac ggccgtaacg atgtcacgtc
     1861 ggaaggcccc ccgctacaat ccgcgccggc accgagctcc cgcgggccgg cggaggcccc
     1921 acgcgggatc gacctgggag gagcgccatg accggcacga cggagatcgc agcacgcctc
     1981 gaccgcagca ccgccgacga ccggctgctc gaccacccgt tctaccgggc gtgggcggac
     2041 gggacgctga cgaccgacga cctccgcttc tacgcggcgc agtacttcaa ccaggtcgag
     2101 gcgttcccgg gctacctcgc ggtgctcgcg gaccgcttgc ccgactcgga ggcgaagacc
     2161 agcgtcctcg agaacctcgc ggacgagcgc gacggcgacc acctcgggct gtggctccgg
     2221 ttcgccgccg cggtcggggc cgactcggtg tcggtgcgcg agaccccggc cgaggacgag
     2281 acgctcgcgt gcatagcggc gttcgaggcc gccgtgcgcg accgctcgcc gtcgttcgcg
     2341 ctcgggatga tctacgggta cgagtcgcag acccccgacg tgtgcgagac gaagatcaag
     2401 ggcctccgcg acctgtacgg cgtcgagggg ccgggcctcg agtacttcga gctgcacggc
     2461 gagctcgacg tcgagcacgc cggcgagctg gccgctgccg tcgccgagct gagctcgtcc
     2521 gaggacgacc tgcgcgacgc cgaggaaggc gccgtcgcgg gggcgcgggc gatccgcggc
     2581 ctgctcgacg gcgtcgcacg cgtacgcggg atcggctgac cctcacgcac gcccggcgcg
     2641 tagccgcgct ctacgacgtc cacgcgaacc cgttcgcgct ggacgcggtg ctcgccgacg
     2701 cgtcgtccgg tgacgtcgac ctcctcgtgt tcgggggcga cctcgcctgg ggggctttcc
     2761 c
//
LOCUS       DASQDO010000092         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_377076, whole genome shotgun sequence.
ACCESSION   DASQDO010000092 DASQDO010000000
VERSION     DASQDO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377076"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VEV43_02810"
     CDS             <1..1783
                     /locus_tag="VEV43_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22481.1"
                     /translation="DAPNVRPRPDGDVDRLLEPPPGTPDLFSYLRANRRYLSTGRGGL
                     PATFLTGAALVAFNVAVLATIVCLLAWPVGRLAATWPVQPELREFAYSRADAQSLAVP
                     LRLWLPGLAGLVAAVASFVVSLVLWDPARKRTLAVAGFFTGGGTLLLAFLVGVPVAMT
                     EVPKTWAALPGDPVEGAGVVAGAVAILLAGAVGWFLVRPFASKVARLGGVLLGLLAFL
                     FAGRVATDAAFGLRSFAWSPSIYLAVAGGFAVFYALADAQSWSWFRLYWLRLRSTFAT
                     TQHASKRARHAPARDGVYPLSVGAEPSWPGYAGRPGPQLVVCAAAQTNRPGVSGIPAE
                     SFTFSDAVDPGYLRGLRARHLGTVSASVAMSGAAFTSAMGRQSLGTTNALLAALNVRL
                     GVWMPNPRYRLDRPLKRPRINYLLKELFGVYDPDDPYVYVTDGGHWENLGLVELVRRR
                     AKWIFCLDASGDPADSFATLQEAIVMARVECGAEIELDPEPLRRGPGGRLPRTAVKTA
                     VIRYHDCGGTGGDDCDTGLLFYGKAMLAQDSPINTLSFSLRDRLYPRYPTYDQFLRDD
                     ELMNLVRLGEWIGRGLALDYDRFRPPE"
     gene            1858..2025
                     /locus_tag="VEV43_02815"
     CDS             1858..2025
                     /locus_tag="VEV43_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22482.1"
                     /translation="MTYVVADGGRTRLVTADFYNYWADKPYATFWRTVRGRDVPVETI
                     RLGPTTSIRPR"
     gene            complement(2037..2297)
                     /locus_tag="VEV43_02820"
     CDS             complement(2037..2297)
                     /locus_tag="VEV43_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22483.1"
                     /translation="MERDPARTDGLSRRRFLRHALVAAWASPMIVTLASHSAQAAAPA
                     TCGKKIGGAGTTACRITSPCGSAAVGCKGSPAAPAGSPCYCI"
     gene            2418..2627
                     /locus_tag="VEV43_02825"
     CDS             2418..2627
                     /locus_tag="VEV43_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22484.1"
                     /translation="MSEAGVYGILSGLMIAGGFVLTVLGGVLWLIDRRFEDRPELMAG
                     KKLTRLGLTVLGAGIVVLLATLLAT"
     gene            2772..2945
                     /locus_tag="VEV43_02830"
     CDS             2772..2945
                     /locus_tag="VEV43_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22485.1"
                     /translation="MVVAVLVAAGLAIAAWAYFRDEGLSCRDWQEAWGREPQTKKLAE
                     LRPEGCREIDIPG"
     gene            3081..3770
                     /locus_tag="VEV43_02835"
     CDS             3081..3770
                     /locus_tag="VEV43_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HYP22486.1"
                     /translation="MPAMRSRPIPEATVARLPVYLRALLDLAEGRGQLTVSSEELAHM
                     AGVNAAKVRKDLSYLGSYGTRGVGYDVEYLLYQITRELGLTKDWPAAIVGIGNLGRAL
                     ASYRGFTERGFRIMALFDVEDSMIGTEVGGLPVRHLDDLKEVAVDQSIAIGIIATPPA
                     AAQEVAERLVDAGVKSILNFAPAVVNVPPDVSVRKVDLSIELQILSFYQQRTQAIATA
                     REANRRLGSGI"
     gene            3803..5083
                     /locus_tag="VEV43_02840"
     CDS             3803..5083
                     /locus_tag="VEV43_02840"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HYP22487.1"
                     /translation="MSIVVVGLNHKTAPVDLLEQLSISEERLGKALHQLATYEHVLEG
                     ALLSTCNRIEAYAVVSKFHGGAQDLRNFFSEFCHVAPEDFADAMYTYHDEGALRHLFR
                     VAAGIDSMVVGESEILGQVRRSYQRAADEGMVHRVLGAAFRQALRVGKRARTETSIGH
                     NPVSVSSAAVELARRASWRDGTLDGRKVTIVGAGKMGRLAAQALAASGAVDVTVVNRT
                     EERGRDLAETFGATSRPFEELEDVLRGSDIVICSTTSTQTVIDRALVERARRGREDEP
                     LFVVDIAVPRDVEPDVGTLPGVVLRDIDDLRDVVEGNLGARLGEVGRVEELIADELDR
                     FSQWQRSTEIAPTAAALVSKADAIRAAEMERIAAHLETMTPAQRDAVDHLSRRMVAKL
                     LHAPLKSARDLAGSKQGQLYLTALRELFELSDEP"
     gene            5095..>5662
                     /gene="hemC"
                     /locus_tag="VEV43_02845"
     CDS             5095..>5662
                     /gene="hemC"
                     /locus_tag="VEV43_02845"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYP22488.1"
                     /translation="MASRGSALALRQSEGVAQALRDAHPGLEVEIRAVETRGDKDQRS
                     FAAIGAKGLFVAEVEQAVVDGRADLAVHSAKDLTAQLAEGCAILCVPRRAPAHDVIVG
                     ADGIEDLPSGATVGTSSMRRRALLAELRPDLDVVDFRGNLDTRLRKVQEGVVDAAILA
                     AAGLARLEVKAGSPIDPDRWVPAPGQGAL"
BASE COUNT          781 a         2017 c         1993 g          871 t
ORIGIN      
        1 cgacgcgccg aacgtgcgcc ctcggcccga cggcgacgtc gaccggctcc tcgagccccc
       61 gccgggcacc ccggacctct tctcgtacct gcgggcgaac cgtcgctacc tctcgacggg
      121 tcgaggcggc ctgccggcga cgttcctcac gggggccgcg ctggtcgcct tcaacgtcgc
      181 ggtgctcgcg acgatcgtgt gcctgctcgc gtggcccgtc gggcgtctgg ccgcgacctg
      241 gccggtccag ccggagctgc gtgagttcgc gtactcgcgc gccgacgcgc agtcgctcgc
      301 cgtcccgctg aggctgtggc tgccggggct ggcgggtctc gtcgcggccg tcgcgtcgtt
      361 cgtcgtctcg ctcgtcctgt gggaccccgc tcgcaagcga acgctcgcgg tcgcggggtt
      421 cttcaccgga ggaggcacgc tcttgctcgc gttcctcgtc ggcgtgccgg tcgcgatgac
      481 ggaggtcccg aagacgtggg ccgcgcttcc gggcgatccc gtagagggag cgggcgtcgt
      541 ggccggggcc gtcgcgatcc tcttggcggg cgccgtgggg tggttcctcg tcaggccgtt
      601 cgcgtcgaag gtagcgcgcc tcggcggcgt gctgctgggg ctgctcgcgt tcctgttcgc
      661 ggggcgcgtg gcgaccgacg cggcgttcgg cctgcgttcg ttcgcgtggt ccccgagcat
      721 ttacctcgcg gtggccggag ggttcgccgt cttctatgcg ctcgctgacg cgcagtcgtg
      781 gtcgtggttc cggctctact ggctgcgcct ccgctccacg ttcgcgacga cacagcacgc
      841 gtcgaagaga gctcgtcatg cccccgcgcg cgacggcgtc tatccgctgt cggtcggcgc
      901 ggagccttcg tggcccggct acgccgggcg gccgggaccg cagctcgtcg tctgcgccgc
      961 ggcccagacg aaccggcccg gggtcagcgg gatccccgcc gagtccttca cgttctcgga
     1021 cgcggtcgat cccggttacc tccgagggct ccgggcgcgg catctcggca ccgtctccgc
     1081 gtccgtcgcg atgtcggggg ccgcgttcac ctccgcgatg ggccgccaga gcctcgggac
     1141 gacgaacgcg ctgctggccg cgctcaacgt ccggctcggt gtgtggatgc cgaacccgcg
     1201 ctaccggctc gaccgcccgc tcaagaggcc gcggatcaac tacctgctga aggagctctt
     1261 cggcgtctac gaccccgacg acccgtacgt ctacgtcacc gacggcgggc attgggagaa
     1321 cctcgggctc gtcgagctcg tccgccggcg ggcgaagtgg atcttctgcc tcgacgcgag
     1381 cggcgacccg gcggactcct tcgcgacgtt gcaggaagcg atcgtgatgg cgcgggtgga
     1441 gtgcggagcc gagatcgagc tcgaccccga gccgctccgc cgcggccccg gcgggaggtt
     1501 gccgaggacg gccgtgaaga ccgcggtgat ccgctatcac gactgcggcg ggacgggcgg
     1561 cgacgactgc gacacggggc tgctcttcta cgggaaggcg atgctcgcgc aggactctcc
     1621 gatcaacacg ctctcgttct cgctgcgcga ccggctctac ccgaggtatc cgacgtacga
     1681 ccagttcctg cgcgacgacg agctcatgaa cctcgtgcgt ctcggcgagt ggatcgggcg
     1741 cggcctcgcg ctcgactacg accgcttccg gcccccggag tagtgcgggg aatgcctatg
     1801 gtcggcctcg attcgcgccg ataggttcag cgagatggtt gccagaggcg ggacccgatg
     1861 acctacgtcg tggccgacgg cggccgaacg cgcctcgtga ccgccgactt ctacaactac
     1921 tgggcagaca agccgtacgc gacgttctgg cggaccgtgc gcggccggga cgtgccggtc
     1981 gagacgatcc gtctcggacc gaccacctcg atcaggcccc gctaggcggc tttcctctag
     2041 atgcagtagc agggcgatcc cgcgggagcc gcggggctgc ccttgcaccc gaccgcggcc
     2101 gagccgcagg gcgacgtgat ccggcaagcg gtcgtcccgg cgccgccgat cttcttcccg
     2161 cacgttgccg gggccgcagc ctgagccgag tgggacgcga gcgtcacgat catcgggctc
     2221 gcccacgcgg cgacgagggc atggcgaagg aacctcctgc gcgaaagtcc gtcggtccgt
     2281 gcggggtccc gttccatggc gccacgctag gtgaggacta gtccgcgcgt ctgtccgtag
     2341 tttcggcact gagaacggct caagttccac tcctcgcgtg acgatctcgc cccgccgcca
     2401 cgcctatcct ggcggcgatg agcgaagccg gcgtctacgg gatcctctcc gggctgatga
     2461 tcgcgggcgg cttcgtgctg accgtcctgg ggggcgtgct gtggctgatc gacaggcgct
     2521 tcgaggaccg gccggagctg atggccggca agaagctgac gcggctgggc ctgacggtcc
     2581 tgggggccgg gatagtggtg ctgctggcga cgctgctggc gacctaggcg acccaggcga
     2641 ggaatatcgc gtcggcgaag ccgacggcga ggatcccgag ggcccaggcc cactcccgga
     2701 tcgagctgct tctatccatg ggacgagctt acgctcgggg ggtgccggaa tcgagtcgag
     2761 gtccgtgggt catggtcgta gccgtcctgg tcgcggccgg actggcgatc gcggcctggg
     2821 cctacttccg ggacgaaggt ctctcctgcc gggactggca agaggcctgg ggccgggagc
     2881 cgcagacgaa gaagctggcc gagctgcgtc ccgaaggctg ccgggagata gacatcccgg
     2941 gctgagcgaa ccaagcaccc ggcgccgccc gcggcggtgc acaaattcac caaccccctt
     3001 ctgtgtcccg gcccctacac cctaaaagtc ctggtcagag cccggattct ggtttgtgat
     3061 atctctcaca agctcgtagg atgccggcca tgcgcagcag gccgatcccc gaagccacgg
     3121 tcgcccggct ccccgtctac ctccgagcgt tgctcgacct cgccgagggc cggggccagc
     3181 tcaccgtctc ctctgaagag ctggcgcaca tggccggcgt caacgccgcc aaggtccgca
     3241 aggacctctc ctacctcggc tcctacggga cccgcggcgt cggctacgac gtcgagtacc
     3301 tcctctacca gatcacccgc gagctcggcc tgacgaagga ctggccggcc gccatagtcg
     3361 gcatcgggaa cctcggccgg gccctcgcct cctaccgcgg cttcaccgag cggggcttcc
     3421 gcatcatggc gctgttcgac gtcgaggact cgatgatcgg gaccgaggtc gggggcctcc
     3481 ccgtgcgcca cctcgacgac ctcaaggagg tcgccgtcga ccagagcatc gccatcggca
     3541 tcatcgccac gcccccggcc gccgcccagg aggtcgccga acgcctcgtc gacgcgggcg
     3601 tgaagtcgat cctgaacttc gccccggccg tcgtgaacgt gccgccggac gtgagcgtcc
     3661 gcaaggtcga cctctcgatc gagctccaga tcctgagctt ctaccagcag cgcacccagg
     3721 ccatcgcgac ggcccgggag gccaaccgcc ggctcggctc gggcatctga gcgagcacgc
     3781 cgacggagta acgttgatcc cgatgtccat cgtcgtagtc ggcctgaacc acaagaccgc
     3841 ccccgtcgac ctgctcgagc agctgtcgat atcggaggag cggctcggca aggcccttca
     3901 ccagctcgcg acctacgagc acgtcctcga gggcgcgctc ctctcgacgt gcaaccgcat
     3961 cgaggcgtat gcagtcgtct cgaagttcca cggcggtgcg caggacctgc gcaacttctt
     4021 ctccgagttc tgccacgtgg cgcccgagga cttcgccgac gcgatgtaca cgtatcacga
     4081 cgagggcgcg ctccggcatc tgttccgcgt cgccgcgggc atcgactcga tggtcgtcgg
     4141 cgagtccgag atcctcggcc aggtccgccg ctcctaccag cgtgccgccg acgagggcat
     4201 ggtgcaccgc gtgctcggcg ccgcgttccg ccaggccctg cgcgtcggaa agcgcgcccg
     4261 caccgagacc tcgatcgggc acaacccggt ctcggtctcg tcggccgcgg tcgagctcgc
     4321 gcgccgcgct tcctggcgcg acggcacgct cgacggccgc aaggtgacga tcgtgggggc
     4381 cgggaagatg ggccgcctcg ccgcgcaggc gctggcggct tcgggcgcag tggacgtcac
     4441 cgtcgtcaac cgcaccgagg agcgcgggcg cgacctcgcc gagacgttcg gcgcgaccag
     4501 ccgtcccttc gaggagctcg aggacgtgct gcgcggcagc gacatcgtca tctgctcgac
     4561 gacctcgacg cagaccgtga tcgaccgggc actcgtcgag cgggcgcggc gcggccgtga
     4621 ggacgagccg ctgttcgtcg tcgacatcgc ggtgccgcgc gacgtcgagc ccgacgtcgg
     4681 gacactgccc ggcgtcgtcc tgagggacat cgacgacctg cgcgacgtgg tcgagggcaa
     4741 cctcggcgcc cgcctgggtg aggtcggacg cgtcgaggag ctgatcgcgg acgagctcga
     4801 ccgcttctcg cagtggcagc gctcgacgga gatcgcacct acggccgccg ccctcgtctc
     4861 gaaggcggat gcgatccggg cggccgagat ggagcgaatc gccgcccacc tcgagacgat
     4921 gaccccggcg cagcgcgatg cggtcgacca cctcagcagg cggatggtcg cgaagctcct
     4981 gcacgcgccg ctgaagtccg cacgagacct cgcggggtcg aagcagggcc agctctacct
     5041 caccgccctg cgggagctct tcgagctctc ggacgagccc tgagctccct cgtcatcgcg
     5101 agccggggct cggccctcgc gctcaggcag tccgaaggcg tcgcgcaggc cttgcgcgac
     5161 gcgcatcccg gcctggaggt cgagatccgc gcggtcgaga cgcgcggcga caaggaccag
     5221 cgttcgttcg cggcgatcgg cgccaagggc ctgttcgtag ccgaggtcga gcaagccgtc
     5281 gtcgacgggc gcgcggactt ggcggttcat tcggcgaagg acctgacggc tcagctcgcc
     5341 gagggctgcg cgatcctctg cgtcccccgg cgcgcgccgg ctcacgacgt gatcgtcggc
     5401 gcggacggca tcgaagacct tccgtcgggc gccacggtcg ggacgtcgag catgagacgc
     5461 cgcgcgctgc ttgcagagct ccgccccgat ctcgacgtcg tcgacttccg cgggaacctc
     5521 gacacgcggc tgcgcaaggt ccaggaaggc gtcgtggacg cggcgatcct cgcggccgcg
     5581 gggctcgcgc gcctggaggt gaaggcgggc tcgccgatcg accccgaccg gtgggtgccg
     5641 gcgccgggcc agggcgcgct cg
//
LOCUS       DASQDO010000093         6568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_438242, whole genome shotgun sequence.
ACCESSION   DASQDO010000093 DASQDO010000000
VERSION     DASQDO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438242"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..112
                     /locus_tag="VEV43_02850"
     tRNA            40..112
                     /locus_tag="VEV43_02850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:73..75,aa:Thr,seq:ggt)
     gene            148..219
                     /locus_tag="VEV43_02855"
     tRNA            148..219
                     /locus_tag="VEV43_02855"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:180..182,aa:Met,seq:cat)
     gene            292..630
                     /locus_tag="VEV43_02860"
     CDS             292..630
                     /locus_tag="VEV43_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22489.1"
                     /translation="MSSRAFCTTCDSEVENAGGFCLLGHRLSACAPPPPPPPPPVTRE
                     PAVAVAVEPEPSRYASSIERTWAELETAPAPSADDPINTFAPPPRMDWGPQRASFRLK
                     LRRPRAEAPA"
     gene            670..831
                     /gene="rpmG"
                     /locus_tag="VEV43_02865"
     CDS             670..831
                     /gene="rpmG"
                     /locus_tag="VEV43_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HYP22490.1"
                     /translation="MASDKRVHVTLECTQCKRRNYITEKNRHNTRDRVELKKYCRWCS
                     GHTAHKETR"
     gene            complement(828..1787)
                     /locus_tag="VEV43_02870"
     CDS             complement(828..1787)
                     /locus_tag="VEV43_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP22491.1"
                     /translation="MKVTVTGGAGFIGSHLVARLVGDGHDVVVLDDLSTGDAASVHPE
                     ATLVTGDVCDAALVRTTVAGSEVVFHLAAHRAIARSIDDPAGTDRTNSQGTVTVLQSA
                     VEAGLRRVVYASSSSVYGDVTVTPVREDAPPAPVSPYAVSKLAGEHYCRVFGHIHGIE
                     TVALRYFNVYGPGQPSDGRYATVIPLFLDALLSGRRPVVYGDGTLTRDFTYVDDVVAT
                     TVAAASAPLGGAVYNVGAGRPYSLLELLAILGALTGVAPDPVHEDARPGDVARTHADP
                     AAAERDLGFRPSVRLEEGLARTVDWFRARRADTVSPRSESVEL"
     gene            1830..2396
                     /locus_tag="VEV43_02875"
     CDS             1830..2396
                     /locus_tag="VEV43_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22492.1"
                     /translation="MTSPPPGPFRMFFVCTGNQARSPIAAAWTARALASLPVEVGSAG
                     TLPRPPSPVLLEAGAIATQLGLDVALHRSSHVASHDLSSADLVVGFELHHVAGAVVDG
                     GAPATKTFLLKELVRLARAVSPPQHADPVARARAVVELADGLRRETGPVLGEEIRDPA
                     GRRIKAFREIVYEVVRTCDEMTALLFGG"
     gene            complement(2393..4855)
                     /locus_tag="VEV43_02880"
     CDS             complement(2393..4855)
                     /locus_tag="VEV43_02880"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYP22493.1"
                     /translation="MSAVPMRGMRIAAAVALVVAGAAVTITAAHAATFTVTRFDDPAP
                     GACDAGDCSLREAVMAANAAPGQDAIQIPEGTFRLTIPSPGEAPLATDASQGDLDVTD
                     PLSISVTCATIDCVMRPGFGATIDAGGRFTVGSGISITDPGIADRVVHVTSSLSLNFV
                     TLSGGTSEQGAGVLVENGTLSIQGGGVSANASNGACCGGGISAVQSTVSLAASTVSNN
                     AVGSCCGGGIYNESSTVTVNGSVTDNDAFGCCGAGIYNWTDAAAGRSASLTLSDARVS
                     GNDVRDCCGGGLYNEAGGPITVTITNSRFENNAASDDCCGGAIYSRPAGNVTASNTTF
                     KANTTAACCGGAIYNEGTFSLDRGVIANNSVVNCCGGGLAGIGSQSSTTLTNVTVSSN
                     TAGTEPGQPALPSAGGLYLDSAGGQMTLTHVTVASNNGTNGASGISNGDVGVPAAGTL
                     TMQRSIVAANQGSNQCRGPITSQGYNIAADNSCSLTHPTDRPSTNAGLEAFNGTFHPL
                     QSTSPAIDAIPTNVCPPPATDQVHTQRPQDRADRPGTGCDVGAIEMAGTSVNPTPTPT
                     PPTPTPGVTPTPTPGVTPTPTPGATPTPTPGVTPTPGVTPTPTPTTGTTASPTASPTA
                     TPTASPDPIDPRCDDPGVVCGTDGSETITGTEDGELIICGDGDDVVNALGGDDTIECG
                     DEGDDGDKDVDAGAGDDEVTCDGTGHDEVDGRSGDDTIRCGAGGDLLRGQKGADVLVC
                     TSGGDLILGGAGPDRIRGGGDGDGLFGGGGRDFVSGGRGGDGVYGQAGRDTLRGGPGR
                     DRCDDRRREDARGCELKGSGRA"
     gene            4948..5196
                     /locus_tag="VEV43_02885"
     CDS             4948..5196
                     /locus_tag="VEV43_02885"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HYP22494.1"
                     /translation="MKVADTMYVEWVEGEAVVLNRESGELHYLNGTAALVFALIQEHG
                     YDGAMTEVRNRFGSTEQIEGELRDLVSDMKEKGILVDG"
     gene            5333..5974
                     /locus_tag="VEV43_02890"
     CDS             5333..5974
                     /locus_tag="VEV43_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22495.1"
                     /translation="MRSTAPGLLGGLPGEPEAPAGALTREGGFSLVAGTADGRVKKLP
                     SLYVNSIQVYAARDVSLVQAKLARAVDAFMASARVSTYMLTACEIGGRRGLFGTDFFN
                     RSIYRQKLKRLGMTFSDDLYVMMSEDGTFYAQDRDPFVPEFVTLQKTSPPRPGADRVS
                     GGFLVYLLSYYRIADIEAPELSRLVALAREIEALRANEPEDLVAALTGAASPA"
     gene            5971..>6568
                     /locus_tag="VEV43_02895"
     CDS             5971..>6568
                     /locus_tag="VEV43_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22496.1"
                     /translation="MTERSELGLSVLDSRFRIVSDDERMIELVRELWEPFVDDDAGGE
                     ARVLTVAKQDAGWRVDAEPNPPIVASDPWVVASTLRNAVSGAAVAAAAPMVPIHGAAV
                     QRDGVFLVLSGPARAGKTTLLLELLDRDWLLVTDDLVPLDLDTLVARPFPKPLSVREP
                     ARWRRFSGAWEVPAWLPDPVVVGLLPATAMPHSEAIQYR"
BASE COUNT          881 a         2356 c         2348 g          983 t
ORIGIN      
        1 cgatcgacct cgagcccgag gtatcctgaa ccggctcagg ccccgttagc tcagacggca
       61 gagcgtctcc atggtaagga gaaggtccac ggttcgattc cgtgacgggg ctcgctcgcg
      121 gtgccacgat cggcagcaaa ccatcggggc ggcgtagctc aggggcagag caagcggctc
      181 ataatcgctg agtcggtggt tcgagtccac ccgccgctac tagaaagatc gcctgcgagc
      241 cctcaattcc cgcgcccgga taccgaagta atcggtggag gtgtctggga tatgtcttcg
      301 agggctttct gcaccacgtg tgactccgag gtcgagaacg ccggcgggtt ctgcctgctc
      361 ggccaccggc tgagcgcctg cgcgcctccg ccgccgcccc caccgccccc cgtcacgagg
      421 gagccggccg tggccgtcgc cgtcgagccc gagccgtccc gctacgcctc gagcatcgag
      481 cggacgtggg ccgagctcga gaccgccccg gccccctcgg ccgacgaccc gatcaacacg
      541 ttcgcacccc cgccgcgcat ggactggggc ccgcaacggg cgtcgttccg cctgaagctc
      601 cgccgcccgc gcgccgaagc cccggcctag ggagcgcccc ggctacggcg gcttgttaga
      661 ctccccgcca tggcaagtga caagcgcgtg cacgtgacgc tcgaatgcac gcagtgcaag
      721 cggcggaact acatcacgga gaagaaccgc cacaacacgc gcgaccgcgt cgagctcaag
      781 aagtactgcc ggtggtgcag cggccacacg gcccacaaag agacccgcta gagctcgacg
      841 ctctccgacc tcggcgacac ggtgtcggcg cggcgcgccc ggaaccagtc gacggtccgc
      901 gccagcccct cttcgagccg caccgagggc cggaacccca ggtcgcgctc cgccgccgcc
      961 ggatcggcgt gcgtgcgggc gacgtcgccc gggcgggcgt cctcgtgcac ggggtcgggg
     1021 gcgacccccg tgagcgcgcc gaggatcgcg agcagctcga gcagcgagta cggccggccc
     1081 gcgcccacgt tgtagaccgc accccccaaa ggcgccgacg cggccgcgac cgtcgtagcc
     1141 accacgtcgt cgacgtaggt gaagtcccgc gtcagcgtcc cgtcgccgta gacgaccggc
     1201 ctccgccccg agaggagcgc gtcgaggaac agcgggatca cggtcgcgta gcggccgtcg
     1261 ctcggctgcc cgggcccgta gacgttgaag taccggagcg cgacggtctc gatcccatgg
     1321 atgtggccga agacccggca gtagtgctcg cccgcgagct tcgacaccgc gtacggcgac
     1381 acgggggccg ggggagcgtc ctcgcggacg ggcgtgacgg tgacgtcgcc gtagacgctg
     1441 ctcgacgacg cgtagacgac ccggcgcagg ccggcttcga cggcggactg caggaccgtc
     1501 acggtcccct ggctgttcgt gcggtcggtg cccgccgggt cgtcgatcga gcgggcgatc
     1561 gcgcgatgcg cggcgaggtg gaacacgacc tcggaccccg cgacggtcgt ccgcacgagg
     1621 gccgcgtcgc agacgtcgcc ggtgacgagc gtggcctcgg gatggacgga cgctgcgtcc
     1681 ccggtcgaga ggtcgtccag cacgacgacg tcgtggccgt cgccgaccag ccgggcgact
     1741 aggtgggacc cgatgaaccc ggctccgccg gtgacggtca ccttcacggc gctaattctg
     1801 ctacgccgca gccgggttag gttcccggcg tgacctcgcc cccacccggc cccttccgga
     1861 tgttcttcgt gtgcacgggc aaccaggcga ggagccccat cgccgccgcg tggacggccc
     1921 gcgccctcgc gtccctgccg gtggaggtcg gttccgccgg gaccctcccg cggcccccga
     1981 gcccggttct cctcgaagcc ggagcgatcg cgacgcagct cggcctggac gtggccctgc
     2041 atcggagcag ccacgtcgcc tcgcacgacc tctcgtcggc cgacctcgtc gtcgggttcg
     2101 agctccacca cgtcgccggc gcggtggtcg acgggggtgc gccggcgacg aagacgttcc
     2161 tgctgaagga gctcgtgcgg ctcgcccggg cggtgtcccc gccgcagcac gcggacccgg
     2221 tggcgcgcgc ccgtgcggtc gtcgagctcg ccgacggcct gcgccgcgag accggcccgg
     2281 tcctcggcga ggagatccgc gaccccgccg gccggcggat caaggcgttc cgcgagatcg
     2341 tgtacgaggt cgtccgtacc tgcgacgaga tgaccgcgct gctgttcggc ggctaggccc
     2401 ggccggaccc tttcagctcg cacccgcgcg cgtcctcgcg ccggcggtcg tcgcaccggt
     2461 cccgcccggg cccgccgcgc agcgtgtcgc ggccggcctg gccgtagacg ccgtcgccgc
     2521 cgcggccccc ggatacgaag tcgcggcccc cgccgccgaa gaggccgtcg ccgtcgcctc
     2581 cgccgcggat ccggtcgggc cccgctcctc cgaggatcag gtcgccaccg gacgtgcaca
     2641 cgagcacgtc ggcacccttc tgcccgcgca gcaagtcgcc tccggcgccg cagcggatcg
     2701 tgtcgtcccc gctccggcca tcgacctcgt cgtgaccggt cccgtcgcag gtgacctcgt
     2761 cgtcaccggc gccggcgtcg acgtccttgt cgccgtcgtc accttcgtcg ccgcactcga
     2821 tcgtgtcgtc gccgccgagc gcgttcacga cgtcgtcgcc gtcgccgcag atgatgagct
     2881 cgccgtcctc ggtgccggtg atggtctcgg acccgtcggt gccgcagacg accccggggt
     2941 cgtcacagcg cgggtcgatc gggtcgggtg aggccgtcgg cgtcgcagtc ggcgaagcgg
     3001 tcggggaggc ggtcgtcccc gtcgtcggcg tcggtgtcgg agtcacaccg ggcgtggggg
     3061 tgacccccgg cgtcggcgta ggcgtagcgc cgggagtcgg tgtcggcgta acgccgggag
     3121 tcggcgtcgg cgtaacgccg ggagtcggcg tggggggcgt gggggtcggg gtcgggttca
     3181 ccgaggtccc ggccatctcg atcgcgccga cgtcgcatcc ggtgccggga cggtccgctc
     3241 ggtcctgtgg acgctgcgtg tggacctggt cggtcgccgg cggcgggcac acgttcgtcg
     3301 ggatcgcgtc tatcgcaggg ctcgtcgact gcaaggggtg gaacgtgccg ttgaacgcct
     3361 cgagccctgc gttcgtcgag ggacggtccg tgggatgcgt caacgagcac gagttgtcgg
     3421 ccgcgatgtt gtagccctgg gacgtgatcg gcccgcgaca ttggttcgaa ccctggttcg
     3481 cggcgacgat cgaccgctgc atcgtcagcg tccccgcggc cggcacgccg acgtccccgt
     3541 tcgagatccc cgacgccccg ttcgtgccgt tgttgctcgc gacggtcacg tgcgtgagcg
     3601 tcatctgccc gcccgccgag tccaggtaca ggcctcccgc agacggcagc gcgggctgac
     3661 ccggctcggt gccggcggtg ttcgagctca ccgtcacgtt cgtcagcgtc gtcgacgact
     3721 gcgagccgat cccggccagg cccccgccgc agcagttcac gacggagttg ttcgcgatca
     3781 ccccgcgatc gagcgagaac gtcccctcgt tgtagatcgc gccgccgcag cacgccgcgg
     3841 tcgtattggc cttgaacgtc gtgttcgacg cggtcacgtt ccccgccggc cgcgagtaga
     3901 tcgcgccgcc gcagcagtcg tcgctcgcgg cgttgttctc gaacctgctg ttggtgatcg
     3961 tgacggttat cggcccgccc gcctcgttgt agaggccacc gccgcagcaa tctctgacgt
     4021 cgttcccgct gacccgggcg tccgacagcg tgagcgaagc gctgcggccc gcggccgcgt
     4081 ccgtccagtt gtagatgccg gcgccacaac agccgaaggc gtcgttgtcg gtgacgctgc
     4141 cgttcacggt gacggtcgag ctctcgttgt agatcccgcc gccgcagcag ctgccgacgg
     4201 cgttgttcga gacggtcgag gcggcgaggc tcaccgtgct ctgcaccgcg gagatccccc
     4261 cgccgcagca cgcgccgttg gaggcgttgg ccgacacgcc cccgccctgg atcgacaagg
     4321 tcccgttctc gacgaggacg cccgcgccct gctcggaggt ccctccggac agcgtcacga
     4381 agttcagcga gagagagctg gtgacgtgga cgacgcggtc ggcgatgccc gggtccgtga
     4441 tcgatatccc cgatccgacc gtgaaacggc cccccgcgtc gatggtcgcc ccgaacccgg
     4501 gccgcatcac gcagtcgatc gtggcgcagg tcaccgagat gctgagcggg tccgtcacgt
     4561 cgaggtcccc ttgggacgcg tcggtcgcca gcggcgcctc tccgggggac gggatcgtca
     4621 gccggaaagt cccctcgggg atctggatcg cgtcttgccc gggggccgcg ttcgccgcca
     4681 tgacggcctc gcggagcgag caatcccccg cgtcacacgc tccgggggcg gggtcgtcga
     4741 accgcgtcac ggtgaaggtt gccgcgtgcg ctgccgtgat cgtcaccgct gccccggcta
     4801 ccaccagcgc gacggccgcg gcgatcctca tgccccgcat cgggactgcg ctcatgggat
     4861 tcctcgcttt ctgcagtgac gccctcgcca acgatggtag tcggcccccg gggagagtcg
     4921 cgcgctcgtt ttcctatact gcgcaccgtg aaggtcgccg acacgatgta cgtcgagtgg
     4981 gtcgagggcg aggccgtcgt gctcaaccgc gagtccggcg agctgcacta cctcaacggg
     5041 acggcggcgc tcgtgttcgc cctgatccag gagcatggct acgacggtgc gatgaccgag
     5101 gtgcgcaacc gcttcggctc gaccgagcag atcgagggcg agctccgcga cctcgtctcc
     5161 gacatgaagg agaagggcat cctggtagat gggtaacgct ccgatcgacg acggcgaggt
     5221 cgtcgtcgac gagccgggcg agccccgcgc ggcggcgggc tgacccgatc ccttcgaagg
     5281 cctggtctgg gccgactcca tctggatacc gacgaaagag gcatggcgcg aggtgcgttc
     5341 gaccgcgccc ggtcttctgg gcgggctgcc gggcgagccc gaggcgccgg ccggcgcgtt
     5401 gacgcgcgag ggcggcttca gcctcgtggc cgggacggcc gacggccgcg tcaagaagct
     5461 cccgtcgttg tacgtgaaca gcatccaggt ctacgcggcg cgcgacgtgt cgctggtcca
     5521 ggcgaagctg gcgcgggcgg tggacgcgtt catggcgtcg gcgcgcgtct cgacctacat
     5581 gctcaccgcg tgcgagatcg gcgggaggcg cggcctgttc gggaccgact tcttcaaccg
     5641 ttcgatctac cgtcagaagc tgaagcgtct cgggatgacg ttctccgacg acctctacgt
     5701 gatgatgagc gaggacggca cgttctacgc gcaggaccgc gacccgttcg tgcccgagtt
     5761 cgtgacgttg cagaagacct cgccgccgcg gccgggcgcg gaccgtgtgt cgggcgggtt
     5821 cctcgtctac ctcctgagct actaccggat cgcggacatc gaggcgcccg agctgtcgcg
     5881 gctcgtcgcg ctggcgagag agatcgaggc cctgcgcgcg aacgagccgg aggacctcgt
     5941 ggcggcgctg acgggggccg cgtcgcccgc gtgaccgagc gctccgagct ggggctgtcg
     6001 gtgctcgaca gccggttccg gatcgtctcg gacgacgagc ggatgatcga gctcgtgcgc
     6061 gagctgtggg agccgttcgt cgacgacgac gccggcggcg aggcccgggt cctcacggtc
     6121 gcgaagcagg acgcgggatg gcgcgtcgac gccgagccga atccgccgat cgtcgcgtcg
     6181 gacccgtggg tggtcgcgtc gaccttgcgg aatgccgtca gcggtgctgc ggtcgccgcg
     6241 gcggccccca tggtccccat ccacggagcc gcggtgcagc gcgacggggt gttcctggtg
     6301 ctgtcggggc cggcgagggc cggcaagacg acgctgctgc tcgagctcct cgaccgtgac
     6361 tggctgctcg tcaccgacga cctggtgccc ctcgacctcg acaccctcgt ggcgcgcccc
     6421 ttcccgaaac cgttgtcggt gcgggagccg gcacggtggc ggcggttctc cggcgcgtgg
     6481 gaggtcccgg cctggctccc cgaccccgtg gtggtcggcc tcctccccgc gacggcgatg
     6541 ccgcactcag aggcgatcca gtaccggc
//
LOCUS       DASQDO010000094         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_927801, whole genome shotgun sequence.
ACCESSION   DASQDO010000094 DASQDO010000000
VERSION     DASQDO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927801"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VEV43_02900"
     CDS             <1..1130
                     /locus_tag="VEV43_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP22497.1"
                     /translation="RGIQTDVYTRADGSGPTVTSLGTGVRVIAVPAGPRERLPKETLT
                     AYLGEFVSGVRAFALAQRLRYDVVHSHYWQSGLAAVPLAAAWGVPLVHSHHTLGKVKN
                     RFLAPGDRPEPPVRLDGEASVIEAADVLVASTDDEWTHLACLYGAHHDKLKTLHPGVD
                     HGVFSPGDRAEARASLRLGDEAVMLYVGRIQRLKGIELAIRAAEQLVPALDQPLRFLI
                     AGGASGAAGEIEVARLSALVDELGLGDVVRFVGAVPHAELPAYYRAADAVVVCSHSES
                     FGLAALEAHSCGTPVVGTPVGGLSHVVADERSGFLVDTRDPAVFAARLKTLLADAHLR
                     ARFSSAAVAAASSFSWDRTAASLQELYECLVREQSPEACTC"
     gene            complement(1379..2287)
                     /locus_tag="VEV43_02905"
     CDS             complement(1379..2287)
                     /locus_tag="VEV43_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HYP22498.1"
                     /translation="MLGRALIWASEKSRIKDFVTQGKLAGVAHRFVAGDHLEDAIQAI
                     KDLNSKGIGGILDLLGEGVTDPAGAEAALAEYGQSVKRIDETGIDTTVSVKLTQLGLA
                     FDKGTCIDYVRRLAAEAQAIGTRIEIDMEQSEYVVDTLDVYRLLTADYPDLRIAMQVY
                     LRRTAVDLETLSALKPRVRLVKGAYAEPPEIALQKRKEIDAQYRFLTEWLFEKGTDPG
                     IATHDSALIEHAQRVARARGVGSDGFEIQMLYGIRQDLQEDLARQGHRVRAYVPYGTD
                     WYPYLMRRMAERPANLRFFLRALSGK"
     gene            2327..>2818
                     /locus_tag="VEV43_02910"
     CDS             2327..>2818
                     /locus_tag="VEV43_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYP22499.1"
                     /translation="MTKLAVLGAGKMGEALIAGLVASGWRKPEDIVATARTQERLDAL
                     AGSYGVQTTLSNPEAVAGAQVVVLAVKPQDAEALLADVSPHVGEDHTLLSVVAAISTA
                     FIESHLAGPVPVVRAMPNTPSIVHEGMAGIAGGKHATDEHVGLAREVLTHVCRVAIFP
                     ERYL"
BASE COUNT          400 a          986 c          972 g          460 t
ORIGIN      
        1 gacgcggaat ccagacagac gtctacacgc gcgccgacgg ctccggcccg accgtgacct
       61 cgctcggaac gggggtgcgg gtgatcgcag tcccagcggg gccccgtgaa cgcctcccga
      121 aggagaccct gaccgcctat ctcggcgagt tcgtctccgg ggtccgcgcc ttcgccctcg
      181 cccagcgcct gcgctacgac gtcgtgcact cgcactactg gcagtcgggt ctggcggccg
      241 taccgctcgc ggccgcgtgg ggcgtcccgc tggtccattc ccaccacacg ctcgggaagg
      301 tcaagaaccg tttcctcgcc ccgggagacc ggccggagcc gccggtccgc ctcgacggcg
      361 aagcgagcgt gatcgaagcc gcggacgtgc tggtcgcgag caccgacgac gagtggacgc
      421 acctcgcctg cctgtacggg gcgcaccacg acaagctgaa gacgctgcac ccgggcgtcg
      481 accacggcgt cttctcgccc ggagaccggg ccgaggcgcg ggcttccctg aggctcggcg
      541 acgaggccgt gatgctctac gtcgggcgca tccagcgcct caagggcatc gagctcgcga
      601 tccgcgccgc ggagcagctc gtgccggcgc tggaccagcc gctcaggttc ctgatagcgg
      661 gcggggccag cggggccgcg ggcgagatcg aggtcgcgcg cctgagcgcg ctcgtcgacg
      721 agctcggcct cggagacgtc gtgcggttcg tcggcgcggt cccccacgcg gagctgcccg
      781 cgtactaccg cgcggccgac gcggtcgtcg tctgctcgca ctccgagtcg ttcggcctgg
      841 cagcgctcga agcgcactcg tgcggaacgc ccgtcgtcgg cacgccggtg ggcggtctct
      901 cgcacgtcgt cgcggacgaa cgctccggct tcttggtcga cacgcgcgat cccgcggtgt
      961 tcgcggcgcg gctcaagacg cttctcgcgg acgcgcactt gcgcgcgcgc ttctcgagcg
     1021 ccgcggtcgc ggccgcgtct tcgttctcgt gggaccgcac cgccgcgtcg ttgcaggagt
     1081 tgtacgagtg cctcgtgcgt gagcagtcgc ccgaagcgtg cacgtgttag cgacgcagcg
     1141 cgactcgcga atatttatcc cgacgcgctg accaggatgt ttaagggtct accaggggtt
     1201 tcgcgttgtt gacgcatctt cgaggcgtcg cctatcttct cgcagtcccg agcggccgga
     1261 agggttcgcc cggaagctcg ctcgggacga caggccccaa aagtccgaag ccgtaacgca
     1321 tcgtcgtgtg tggggaagtg cacggggacg cgagacggcc gaggtttttg gggcctgact
     1381 atttcccgga aagagccctc aagaagaacc gcaggttcgc agggcgctcg gccatgcgcc
     1441 gcatcaggta cgggtaccag tcggtgccgt agggaacgta cgcgcgcact cgatgcccct
     1501 gccgcgcgag gtcttcctgc aggtcctgtc tgatcccgta cagcatctgg atctcgaacc
     1561 cgtcgctgcc gacgccgcgt gcgcgtgcga cgcgctgcgc gtgctcgatc aacgcggagt
     1621 cgtgcgtcgc gatgcccggg tccgtcccct tctcgaacag ccactcggtc aggaagcggt
     1681 actgcgcgtc gatctccttg cgcttctgga gcgcgatctc cgggggctct gcgtacgcgc
     1741 ccttgacgag ccgcacccgg ggcttgagcg ccgacagcgt ctcgaggtcg acggcggtcc
     1801 ggcggaggta gacctgcata gcgatccgga ggtcggggta gtcggcggtg aggagccggt
     1861 agacatcgag cgtgtcgacg acgtactccg actgctccat gtcgatctcg atgcgggtcc
     1921 cgatggcctg ggcctcggcg gcgagccggc ggacgtagtc gatgcacgtc cccttgtcga
     1981 aggcgagccc gagctgcgtc agcttcacgg acacggtcgt gtcgatgccg gtctcgtcga
     2041 tccgcttgac cgactggccg tactccgcca gggcggcctc cgcccccgcc gggtccgtca
     2101 ccccctcgcc gaggaggtcg aggatcccgc cgatgccttt ggagttgagg tccttgatcg
     2161 cctggatcgc gtcctcgagg tggtcgccgg cgacgaacct gtgcgcgacg cccgcgagct
     2221 tcccctgcgt gacgaagtcc ttgatccggc tcttttccga agcccagatc agcgcacgac
     2281 cgagcaccag cgtttcccct ctctaggttg ctgacattat gtcggcatga cgaagctggc
     2341 cgtgctgggc gccgggaaga tgggcgaggc gctgatcgcc ggactggtcg cgtcgggatg
     2401 gcgcaagccc gaggacatcg tcgcgaccgc gcgcacgcag gagcgcctcg acgccctggc
     2461 cggctcgtac ggggttcaga ccacgctgag caaccccgaa gccgtcgcgg gggcccaggt
     2521 cgtcgtgctg gctgtgaagc cccaggacgc cgaggccctg ctcgccgacg tctcgccgca
     2581 cgtcggcgag gaccacacgc tgctttcggt ggtcgccgcg atctcgacgg cgttcatcga
     2641 gtcgcacctc gccggccccg tcccggtcgt ccgcgcgatg ccgaacaccc cgtcgatcgt
     2701 ccacgagggc atggccggga tcgccggagg aaagcacgcg accgacgagc acgtcggtct
     2761 cgcacgagag gtgctcaccc acgtctgtcg agtagccatc ttccccgagc gctacctc
//
LOCUS       DASQDO010000095         9869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_989494, whole genome shotgun sequence.
ACCESSION   DASQDO010000095 DASQDO010000000
VERSION     DASQDO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989494"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..991)
                     /gene="rlmB"
                     /locus_tag="VEV43_02915"
     CDS             complement(59..991)
                     /gene="rlmB"
                     /locus_tag="VEV43_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HYP22500.1"
                     /translation="MASGRDGLSDRDRRGQRAGRREPGDHNRGRRPGKTDERARSDRP
                     EPDDDGLLWGRRPVLEALGSGRGVERIIVSRDLAPSGIVGEIRKRAEASAVPLRLVPK
                     AEVDKAAGRVNHQGVVAFTAKYRYTPLEDILAAPDQRVLFLDGVTDPHNPGSLLRSAD
                     GAGFHGVVVPSRRSAGVTGAVRRVSAGAAEVVPVARVNSLGNALDQAKKAGLWIVGLD
                     AAAPEDVWTSELLEPPVGLVLGAEDRGISAGVAGRCDGLVTIPSAGRVASLNVAVAGA
                     IAMFEVSRKAALRVRSVTENGGPADGTRSFGRFG"
     gene            complement(967..2361)
                     /gene="cysS"
                     /locus_tag="VEV43_02920"
     CDS             complement(967..2361)
                     /gene="cysS"
                     /locus_tag="VEV43_02920"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYP22501.1"
                     /translation="MGVRIYDSKLRKVVPLETRDEGRVAMYCCGPTVYNYIHIGNART
                     LLWFDAIRHYLSYRGYDVTYVMNYTDVDDKIIERANAEGIPPDGITTKYTKAFEEDMA
                     ALGARPADIVCRATDHIEHMVEAIGGLIDNGLAYEANGDVWYAVEKFDGYGSLSGRSL
                     DDMRAGERVEPNEHKRHPLDFALWKSAKEGEPSWPSPWGPGRPGWHIECSVMSTKYLG
                     MGFDLHGGATDLIFPHHENELAQAEGLAGDGPFVRHWMHAGLVQMDAEKMSKSLGNFV
                     LAHDLLGRYDGEALRYWILAGSYRSQASFTEESLEDARSSYARWRNFADSVVREAGDA
                     APDKAPEGDPYVQRFVEAMDDDFNSAGAFAVVHDLVKEGNKAAAAGGEGLGPLVGAFR
                     ELTGVLGFRFPSSQDASGIVEGLLDFLLEMREEARAEKAYARSDAIRDRLTSLGVTIE
                     DTAAGPRWRLGGTG"
     gene            complement(2371..2856)
                     /gene="ispF"
                     /locus_tag="VEV43_02925"
     CDS             complement(2371..2856)
                     /gene="ispF"
                     /locus_tag="VEV43_02925"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HYP22502.1"
                     /translation="MTRTGIGFDAHAFAPADDPRPLVVGGVTIPDHPGLAGHSDADVL
                     SHAVADALLGAAALGDLGSRFPADELWKDASSLAILEETAAQVEDAGYRIVNVDVSVV
                     AEAPRLAPHRDAMRRNVAGALGVEEAAVSVKATTTDGLGFTGRGEGMAAMAVATIERA
                     T"
     gene            complement(2853..3614)
                     /gene="ispD"
                     /locus_tag="VEV43_02930"
     CDS             complement(2853..3614)
                     /gene="ispD"
                     /locus_tag="VEV43_02930"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HYP22503.1"
                     /translation="MQRGRGREDARRGPRIAPSVAGIIAAGGRGRRAGFLGPTEPPKQ
                     FRHLGGLPLIGWSVRLLADAGCDPVVVVVPEGSWTDQLSGYDVRMVLGGDERQKSVRN
                     GLDLVHTDYVVVHDAARPFATLGLLRAVLDALTTDRLGDRPASRGAHGAAPAIPVADT
                     LKEVRDGRVIRTVERSSLWAVQTPQAFQTGALKSVHDKARVDGFVGTDDAQLVERYGG
                     VMRIVEGARDNIKLTYPEDWRVAEAMVAASRPREL"
     gene            complement(3566..4048)
                     /locus_tag="VEV43_02935"
     CDS             complement(3566..4048)
                     /locus_tag="VEV43_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HYP22504.1"
                     /translation="MAFKVGDKVVYPHHGAATIESLEDKELMGETREYFILKLAYGEL
                     TLMVPVDSTEEVGLRQVTPAKEVPKVLKVLKKNEPTTNTTNWSRRFKANVEKLRSGDI
                     YQVADVVRSLHQRDKEKGLAAGEKRMLTKARQILVSELTFSKDCNEEEAEKMLDEVLG
                     "
     gene            complement(4203..5585)
                     /gene="radA"
                     /locus_tag="VEV43_02940"
     CDS             complement(4203..5585)
                     /gene="radA"
                     /locus_tag="VEV43_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HYP22505.1"
                     /translation="MATRVAPFRCTSCSHESPRWFGRCPGCGDWSTAETAPAPPGGGR
                     GGARRGVHGAAAPALATLAAPAAPSPRVGSGNDEVDRVLGGGFVPGEVVLLAGEPGVG
                     KSTLVLQVLAGVAASGGRVLLTTGEESLAQVSLRAVRLGLDAESITAVAEPSLPGILA
                     ACASHRPELLVVDSIQTVEDPAFEQPAGSIVQVRECAATLARHAKATNTIVVLVGHVT
                     KDGSVAGPRTLEHLVDAVVSLEGDRSASLRLLRASKNRFGSCEETGVFTMERDGLVVV
                     PDPSSMLLADRCEGVAGSLVVPGLEGSRPVLVELQALATPSDLPQPRRVALGVDARRL
                     SMLGGVLEKAGVRLGKRDLFTSAAGGLAVREPAADLALCLAIESAVSDVPVDARTVAL
                     GEVGLGGEIRRVPGTERRLAEAARLGFRRAIVPAGAEVSGATIEVVRAARLRDAFTAA
                     AAHPARSTAG"
     gene            complement(5620..5769)
                     /locus_tag="VEV43_02945"
     CDS             complement(5620..5769)
                     /locus_tag="VEV43_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22506.1"
                     /translation="MTEQERRRRIEKVKESARLTDEMLARNEAALDLALARLRLAAER
                     LTQAR"
     gene            complement(5779..6180)
                     /locus_tag="VEV43_02950"
     CDS             complement(5779..6180)
                     /locus_tag="VEV43_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22507.1"
                     /translation="MAEAKRRRREYWAGFATVMAATAVLFFRWWPVPQEVEGSFLDAV
                     VQDPVMVGALRLTIFAGTLYALASIPALVVGGRWARSVGTAGFVADEAQVPASVEEAD
                     RTIANLRVVVSRLLRERDELLSLMEAETNPD"
     gene            complement(6215..9106)
                     /locus_tag="VEV43_02955"
     CDS             complement(6215..9106)
                     /locus_tag="VEV43_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYP22508.1"
                     /translation="MTPLLLVAIALGGAGYQMVRRPMLRRLAVRDALRRRNETLLVIA
                     GSLLGTAIITGSFIVGDTLDSSIRVTARTQLGPIDEGISVPDPERAAEIASGIRELED
                     PRIDGVLSMLRAPAAMSVARGGDVLAEPSAQLVEVDFGDARSFGDDPAATGISGETPG
                     DGEVAVSEDLAETLAVDVGDEVTASLYGKSLSLEVVRVLPRLGVAGFWTGFESVSPNA
                     FVAPGTLADVTGAEVPQGASPPVTSVLVSNRGGVDEGAALSEEVAELIRERVTGTLNA
                     LQVETYKQDVLDAAEEQGQMFSELFLGIGSFAVIAGVLLLVNIFVMLAEERKSQLGML
                     RAVGLRRSDLVRSFYIQGGIYSVPSGILGALLGIGVGYAIVVVAAPIFGGIGDFSLDL
                     RFSMEPASIVTGLCTGILIALVTVFGTSLRISRINIIRAIRDLPDPKQEKTRTSALVA
                     GCLAAAGAGAWFVASLGSEEGWVGLLLGPPLFAFALLPLLRRLVARRPALLGVALASL
                     LWGIFGNALLGGRVFDGGDIFVFVLQGVLLNVSGVILLTQTQENLGNVLHRFAAGSLA
                     LRLGLAYPLARRVRTGLTLGMFSLVVFTMVFIAVLARIFGGQVEETARREGGGYDVLV
                     DSAASNPPRPEAIASVEGVEAAAAVDYGTAVFALPDAPADAGGDPTHWTVTGIDEGFV
                     EGGPPALEERPARFATDEEAWRALLDDPDAVIVPEFFFVEGGGPQGGVVEVGDELAVS
                     NPLTGEAAAKEIIALSGNDFAFSGVYMHKTGEFRELLKGAVTASRFFVVAEGSEADAR
                     RTATRLQGELVENGVEAETFRTRVEEFGRVNLQFLRLMQGYLALGLLVGIAGLGVVMV
                     RAVRERRRQVGVLRSLGFLPRMVRRAFLLESGFTAIEGILLGTILSLVTASQLVANGD
                     FGQGVEFQIPWTDVTILCVVSLGASLLATTWPARQASRIPPAVALRVED"
     gene            complement(9103..9765)
                     /locus_tag="VEV43_02960"
     CDS             complement(9103..9765)
                     /locus_tag="VEV43_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP22509.1"
                     /translation="MPSRLQPAGVVEALRDLDLTIPRGEFVAVMGPSGSGKTTLLNCL
                     SGLDDVDGGTVVVEGQDIHALSDAKRSRHRAEKMGFIFQSFNLIPVFTAAENVELPLL
                     LAGAAPKEARRRAEETLERVGLGHRKGHRPNELSGGEQQRVTIARALAGGPAIVWADE
                     PTGNLDTETAGSIVELLEELNRDGLTLVLVTHDPAIGARAGRLVQMRDGAIVSEERPA
                     AR"
BASE COUNT         1449 a         3571 c         3449 g         1400 t
ORIGIN      
        1 caggagggtt tcgatcgcct tgtcgtcgcc ccctcgggcg cgatctacta gctcttcgtc
       61 acccgaatcg gccaaagctt ctagtgccat ccgcaggtcc cccgttttcg gtgacagagc
      121 gtacccgaag ggccgccttc cgcgacacct cgaacatggc gatcgcgccc gcgacggcga
      181 cgttcaacga cgccactctc cccgccgacg ggatcgtcac gaggccgtcg caacggcccg
      241 ctacgcccgc agagatgccc cggtcctcgg ctcccaacac cagcccgacg ggtggctcga
      301 gcaactcgct cgtccagacg tcctcggggg ccgcggcgtc gaggccgacg atccacagcc
      361 ccgccttctt cgcctggtcg agggcgttgc cgaggctgtt cacgcgggcg acggggacga
      421 cctcggccgc gcccgccgag acgcggcgga cggcgccggt cacgcccgcg gacctccgcg
      481 agggcacgac gacgccgtgg aacccggccc cgtcggcgga ccgcaggagc gagccggggt
      541 tgtgggggtc ggtgacgccg tcgaggaaca ggacccgctg gtcgggggcc gcgaggatgt
      601 cctcgagcgg cgtgtagcgg tacttcgcgg tgaaggcgac cacgccctgg tggttgaccc
      661 ggccggcggc cttgtcgacc tcggccttcg ggacgagcct cagggggacc gcagaggctt
      721 cggcgcgctt gcggatctcc ccgacgatgc ccgacggcgc gaggtcgcgg gagacgatga
      781 tcctctcgac gccccggccg ctgccgagag cctcgagcac cgggcgccgc ccccacagga
      841 gcccgtcgtc gtccggctcc ggccggtcgc tgcgggcccg ctcgtccgtc ttccccggac
      901 gccggccgcg gttgtggtca ccgggctcgc gacgccccgc gcgctgcccg cgccggtcgc
      961 ggtcgctcag cccgtcccgc ccagacgcca cctggggccc gccgcggtgt cttcgatcgt
     1021 cacgccgagg gaggtgagcc ggtcgcggat cgcgtcggag cgcgcgtagg ccttctcggc
     1081 cctcgcctcc tcgcgcatct ccagcaggaa gtcgagcagc ccctcgacga tcccggacgc
     1141 gtcctgcgac gacgggaagc ggaagccgag gacgccggtg agctcgcgga acgcgccgac
     1201 gagggggccg aggccctccc cgccggccgc cgcggccttg ttgccctcct tgacgaggtc
     1261 gtggacgacg gcgaacgcgc ccgcggagtt gaagtcgtcg tccatcgcct ctacgaagcg
     1321 ctgcacgtag gggtcgcctt cgggggcctt gtcgggggcc gcgtcgccgg cctccctcac
     1381 gacggagtct gcgaagttcc tccagcgcgc gtacgacgag cgggcgtcct cgagcgactc
     1441 ctcggtgaac gacgcctggc tccggtacga ccccgcgagg atccagtagc ggagggcctc
     1501 gccgtcgtac cgcccgagca ggtcgtgcgc gagcacgaag ttgccgagcg acttcgacat
     1561 cttctcggcg tccatctgca cgaggccggc gtgcatccag tgccgcacga aagggccgtc
     1621 cccggccagg ccttcggcct gggccagctc gttctcgtgg tgcgggaaga tcaggtcggt
     1681 cgctccaccg tggaggtcga accccatccc gaggtacttc gtcgacatca cggagcactc
     1741 gatgtgccag ccggggcggc ccggccccca cggcgacggc cacgacggct cgccctcctt
     1801 cgccgacttc cacagcgcga agtcgagggg gtgccgcttg tgctcgttcg gctcgacgcg
     1861 ttcgccggcg cgcatgtcgt cgaggctccg gcccgagagc gatccgtagc cgtcgaactt
     1921 ctcgactgcg taccagacgt cgccgttcgc ctcgtacgcg aggccgttgt cgatcaggcc
     1981 cccgatcgcc tcgaccatgt gctcgatgtg gtccgtggcg cggcagacga tgtcggccgg
     2041 cctcgcgccg agcgcggcca tgtcctcctc gaacgccttt gtgtacttcg tcgtgatccc
     2101 gtccggcggg atgccctcgg cgttggcgcg ctcgatgatc ttgtcgtcga cgtccgtgta
     2161 gttcatgacg taggtgacgt cgtagccgcg gtagctcagg tagtgcctga tcgcgtcgaa
     2221 ccacagaagc gtgcgtgcgt tgccgatgtg gatgtagttg tagacggtgg gcccgcagca
     2281 gtacatcgcg acgcggccct cgtcgcgcgt ctcgagcggc acgaccttgc ggagcttcga
     2341 gtcgtagatc cgtacaccca tcgccgtacc ttaggtcgct cgctcgatcg tggctaccgc
     2401 catggccgcc atcccctcgc cgcgtccagt gaatccgagg ccgtcggtcg tcgtggcctt
     2461 caccgagacg gcggcctcct cgacgccgag ggcgccggcg acgttgcggc gcatcgcgtc
     2521 gcggtgtggg gcgagccgcg gcgcctccgc gacgaccgag acgtccacgt tcacgatccg
     2581 gtagcccgcg tcctcgacct gcgccgcggt ctcctcgagg atcgcgaggc tcgaggcgtc
     2641 cttccacagc tcgtccgcgg ggaaccggga gccgaggtcg ccgagggccg cggcccccag
     2701 cagcgcgtcg gcgacggcgt ggctgagtac gtccgcgtcg gagtggccgg cgagacccgg
     2761 gtggtcgggg atcgtgaccc cccctacgac cagagggcgc gggtcgtcgg cgggcgcgaa
     2821 ggcatgtgcg tcgaagccga tccccgtcct cgtcatagct cgcgggggcg gctggccgcg
     2881 accatcgcct cggcgacccg ccagtcctcg gggtaggtga gcttgatgtt gtcgcgggcg
     2941 ccctcgacga tcctcatcac gccgccgtag cgctcgacga gctgcgcgtc gtcggtcccg
     3001 acgaagccgt cgacacgggc cttgtcgtgg acggacttga gcgccccggt ctggaaggcc
     3061 tgcggcgtct ggacggccca caggctcgat cgctcgacgg tgcggatcac acggccgtcg
     3121 cggacttcct tcagcgtgtc cgccacgggg atcgcggggg ccgcgccgtg tgctcccctc
     3181 gacgccggcc gatccccgag gcgatcggtc gtcagcgcgt cgaggacggc gcgcagcagg
     3241 ccgagggtcg cgaacgggcg ggccgcgtcg tggacgacga cgtagtcggt gtggacgagg
     3301 tcgaggccgt tgcggacgga cttctggcgc tcgtcgccgc cgaggaccat cctcacgtcg
     3361 tagccgctga gctggtcggt ccacgacccc tcgggcacaa cgacgacgac gggatcacac
     3421 ccggcgtccg cgagcaggcg gacggaccat ccgatgagcg ggagcccgcc gaggtggcgg
     3481 aactgcttgg gaggctcggt gggaccgagg aacccggctc gccgccccct tccccccgcg
     3541 gcgatgatgc cagctacgga aggcgctatc cgaggacctc gtcgagcatc ttctcggcct
     3601 cttcctcgtt gcagtcctta gagaaggtga gctcggagac gaggatctgg cgggccttcg
     3661 tgagcatccg cttctcgccg gcggcgaggc ccttctcctt gtcgcgctgg tggaggctgc
     3721 ggacgacgtc ggcgacctgg tagatgtcgc ccgagcggag cttctcgacg ttcgccttga
     3781 agcgccgcga ccagttcgtc gtgttcgtcg tgggctcgtt cttcttgagc accttgagga
     3841 ccttcggcac ttccttcgca ggggtgacct ggcgcagccc gacctcctcg gtggagtcga
     3901 ccggcaccat cagcgtcagc tcgccgtacg cgagcttgag gatgaagtac tcacgggtct
     3961 cgcccatgag ctccttgtcc tcgagactct cgatggtggc ggcaccgtgg tggggataga
     4021 cgaccttgtc accgactttg aaagccacta cgacgttcct ccctgcgtgc gatctgccga
     4081 gcctcggatg cggccggggg cgagacaggg ggcggagata ccgttgggat acggcccgag
     4141 aacccgtcct gtcgcctcac caagggtagc acggacgcct tccgcgggcc ctcgggacgc
     4201 gctcatccgg ctgtgctccg cgccggatgt gcggccgcgg cggtgaacgc gtcgcgcagg
     4261 cgtgctgcgc gcacgacctc gatcgtcgcg ccggagacct ctgcccccgc gggcacgatc
     4321 gcgcggcgga agccgagccg cgccgcctcg gcgagacgcc gctcggttcc ggggacgcgc
     4381 cgtatctcgc cgccgagacc gacctcgccg agcgcgaccg tgcgcgcgtc gaccggcacg
     4441 tccgacaccg cggactcgat cgcgaggcac agcgcgaggt cggccgcggg ctcgcgcacc
     4501 gctaggcctc ccgccgcgga cgtgaagagg tcgcgcttgc cgaggcgcac gcccgccttc
     4561 tccagcacgc cgcccagcat cgagagacgg cgcgcgtcga cgccgagcgc gacgcggcgt
     4621 ggttgtggca ggtcgctcgg ggtcgcgagg gcctggagct cgacgaggac gggccgcgat
     4681 ccctcgagcc cggggaccac gagcgacccg gctacgccct cgcaacgatc ggcgaggagc
     4741 atcgacgacg ggtcggggac gacgacgagg ccgtcgcgct ccatcgtgaa gacgccggtc
     4801 tcctcgcacg acccgaagcg gttcttcgac gcccggagca ggcggaggct cgcggaacgg
     4861 tcgccctcga gcgagacgac cgcgtcgacg aggtgctcga gcgtgcgggg gccggcgacc
     4921 gaaccgtcct tcgtgacgtg gccgacgagc acgacgatcg tgttcgtcgc cttggcgtga
     4981 cgggcgagcg tggcggcgca ctcgcggacc tggacgatcg atcccgcggg ctgctcgaag
     5041 gccgggtcct cgacggtctg gatcgagtcg accactagga gctcggggcg gtgggacgcg
     5101 caggcggcga ggatgcccgg aagcgagggc tcggcgaccg ccgtgatgga ctcggcgtcg
     5161 aggccgagcc gcacggcccg gagcgagacc tgggccagcg actcctcgcc cgtcgtcaga
     5221 aggacgcggc cgccgctcgc cgcgacgccg gcgagcactt ggagtacgag cgtggacttg
     5281 ccaacgccgg gctcgcccgc gaggagcacg acctcgcccg ggacgaagcc gccgccgagg
     5341 acgcggtcga cctcgtcgtt gccgctgccg acccggggcg agggcgcggc gggggccgcg
     5401 agcgtggcga gggccggagc tgcggcgccg tggacgcccc gtcgggcgcc gccccggccc
     5461 cctccgggcg gggccggggc ggtctcggcg gtgctccagt cgccgcagcc gggacagcgg
     5521 ccgaaccagc gaggtgactc gtgggagcag gacgtgcaac gaaaaggtgc gacgcgggta
     5581 gccatggccc gacggtacag agggggtgtg acgaaaaagc tagcgggcct gcgtcaaccg
     5641 ctcggcggcg agcctgaggc gagcgagcgc gagatcgagc gccgcctcgt ttcgggcgag
     5701 catctcgtcc gtgagacgag cggactcctt gaccttctcg atccttcggc gacgctcttg
     5761 ttcggtcacg atctcatcct agtcagggtt cgtctctgct tccatgagtg acaacaactc
     5821 atcgcgctcc cggagcaggc ggctgacgac cacgcgaagg ttcgcgatcg ttcgatccgc
     5881 ttcctccacg gatgcgggga cctgcgcctc gtccgccacg aacccggcgg tcccgacgct
     5941 tcttgcccac cgaccaccga ccacgagcgc ggggatgctc gcgagggcat agagcgtgcc
     6001 cgcgaagatc gtcagcctca gagcgccgac catcacgggg tcttgcacca ccgcgtcgag
     6061 gaacgaccct tcgacctcct gggggaccgg ccaccacctg aagaagagga cagcggtcgc
     6121 ggccatgacc gttgcgaatc ccgcccagta ctcgcgtcgc cgccgctttg cttccgccat
     6181 gggaccgagg ctagtgccgg ggtgcgacag gaaatcagtc ctctacgcgg agggctaccg
     6241 ccggcgggat cctcgacgct tgccgggccg gccaggtcgt cgccagtagc gacgccccca
     6301 gggacaccac gcacaggatc gtcacgtccg tccacgggat ctggaactcc acgccctgcc
     6361 cgaagtcgcc gttcgccacc agctgcgacg ccgtcaccag cgacaggatc gtccccagca
     6421 ggatcccctc gatcgccgtg aaacccgact ccagcaagaa cgcgcgccgg accatcctcg
     6481 gcaggaagcc cagcgaccgc agcaccccga cctgccgccg gcgttcgcgc acggccctca
     6541 ccatcacgac gccgagcccc gcgataccca ccagcaagcc cagcgcgagg taaccctgca
     6601 tcagcctcag gaactgcagg ttcacgcggc cgaactcctc gacgcgcgtg cggaacgtct
     6661 cggcctcgac gccgttctcg acgagctctc cctgcaggcg cgtcgccgtc cggcgcgcgt
     6721 ccgcctccga gccctccgct actacgaaga agcgcgacgc cgtgaccgcg cccttcagca
     6781 gctcgcggaa ctcgccggtc ttgtgcatgt acacaccgga gaacgcgaag tcgttgccgg
     6841 acaaggcgat gatctccttc gccgcggcct cgcccgtcag cggattgctc actgcgagct
     6901 cgtcgccgac ctcgacaacg ccgccctgcg ggcccccgcc ctcgacgaag aagaactcgg
     6961 gcacgatgac cgcgtccgga tcgtcgagca acgcgcgcca cgcctcctcg tcggtggcga
     7021 agcgcgccgg gcgttcctcg agcgcgggcg ggccgccctc gacgaagccc tcgtcgatcc
     7081 ccgtcacggt ccagtgcgtg ggatcgccgc cggcgtcggc cggcgcgtcg ggaagagcga
     7141 acaccgcggt cccgtagtcc accgcggccg cggcctccac cccttccacc gacgcgatcg
     7201 cctccgggcg tggggggttg ctggcggccg agtcgacgag cacgtcgtat ccgccgccct
     7261 cgcggcgcgc ggtctcctcg acctggcccc cgaagatgcg cgcgagcact gcgatgaaca
     7321 ccatcgtgaa cacgacgagc gagaacatcc ccaacgtgag cccggtgcgc actcggcgcg
     7381 cgagcgggta ggcgaggccc agtcgcaacg ccaggctccc cgccgcgaag cgatgcagca
     7441 cgttgccgag gttctcctgc gtctgggtaa gcaggatcac gccggagacg ttgagcagca
     7501 cgccctgcag cacgaacacg aagatgtcgc cgccgtcgaa gaccctgccg ccgagcaacg
     7561 cgttgccgaa gatcccccac agcagagacg cgagcgcgac gccgagcagc gcgggacggc
     7621 gcgcgacgag ccggcgcagc agcgggagga gagcgaacgc gaacagcggg gggccgagca
     7681 ggagaccgac ccatccttcc tcggacccga ggctcgccac gaaccacgcc ccggcccccg
     7741 ctgcggccag acagcccgcg acgagcgccg acgtgcgcgt cttctcctgc ttcgggtcgg
     7801 ggaggtcgcg gatagcccgg atgatgttga tgcggctgat gcgcaggctc gtgccgaaca
     7861 ccgtcacgag cgcgatcagg atgcccgtgc acaaccctgt gacgatgctc gccggttcca
     7921 tcgagaacct gaggtcgagc gagaagtcgc cgatgccgcc gaagatcggg gcggcgacca
     7981 cgacgatcgc gtagccgacg ccgattccta gcagcgcgcc gaggatcccg gacggcaccg
     8041 agtagatgcc tccttggatg tagaagctgc gcacgaggtc cgaccggcgc agcccgaccg
     8101 cgcgcagcat ccccagctgt gacttccgct cctccgcgag catcacgaag atgttcacga
     8161 gcagcagcac gccggcgatg acggcgaacg agccgatgcc gaggaacagc tcggagaaca
     8221 tctgaccctg ctcctccgcc gcgtcgagga cgtcctgctt gtaggtctcg acctgcagcg
     8281 cgttcagcgt tcccgtcacg cgttcgcgga tcagttccgc gacctcctcg ctgagcgcgg
     8341 ccccctcgtc gaccccgccg cggttcgaga cgaggaccga cgtgaccggc gggctcgccc
     8401 cctgcgggac ctcggccccc gtcacgtccg cgagcgtgcc cggcgcgacg aaggcgttcg
     8461 gcgagacgct ctcgaagccc gtccagaagc cagcgacgcc gagccggggg aggacgcgca
     8521 cgacctcgag cgacagcgac ttgccgtaga gcgacgccgt cacctcgtcg ccgacgtcga
     8581 ccgcgagcgt ctcggcgagg tcctccgaca ccgcgacctc gccgtcgccg ggggtctcgc
     8641 cgctgatccc ggtcgcggcc gggtcgtcgc cgaacgagcg cgcgtcaccg aagtcgacct
     8701 cgacgagctg cgcagacggc tccgccagca cgtccccgcc gcgtgcgacc gacatggccg
     8761 ccggcgcacg gagcatcgac aggacgccgt cgatccgagg gtcttcgagc tcgcgtatgc
     8821 cggaggcgat ctccgcggct cgctccgggt cgggcacgga gatgccctcg tcgatcgggc
     8881 cgagctgcgt gcgcgccgtg acgcggatgg acgagtcgag cgtgtcgccg acgatgaagc
     8941 tccccgtgat gatcgcggtg ccgagcaggc ttcccgctat gacgagcagc gtctcgttac
     9001 gtcggcggag cgcgtctcgc accgcgagcc gtcgcaacat cggccggcgg accatctggt
     9061 atcccgcgcc gccgagggcg atcgcgacca gcaggagagg cgtcatcgcg cggccggacg
     9121 ttcctccgac acgatcgccc cgtcgcgcat ctggacgagc cgcccggcgc gcgcgccgat
     9181 agcagggtcg tgcgtcacga ggacgagcgt gaggccgtcg cggttgagct cttcgagcag
     9241 ctcgacgatc gacccggcgg tctcggtgtc gaggttcccg gtcggctcgt ccgcccacac
     9301 gatcgcgggc cctcccgcga gagcgcgggc gatcgtgacg cgctgctgct cgccgccgga
     9361 gagctcgttc gggcggtgtc ccttgcggtg tccgagcccg acgcgctcga gggtctcctc
     9421 cgcccgccgg cgcgcttcct tgggagccgc accggcgagc agcagcggga gctcgacgtt
     9481 ctcggccgcg gtgaacaccg ggatcaggtt gaacgactgg aagatgaagc ccatcttctc
     9541 ggcgcggtgg cggctgcgct tcgcgtcgga gagcgcgtgg atgtcctggc cctcgacgac
     9601 gaccgtgcca ccgtcgacgt cgtccagccc ggatagacag ttgaggagag tggtcttgcc
     9661 cgagcccgac gggcccatga cggcgacgaa ctcaccgcgc gggatcgtga ggtcgaggtc
     9721 gcgcaacgcc tccacgacgc cggccggctg gagcctcgac ggcacgacgg ggagcgtctt
     9781 ctactccgga ctcacgccgc ccgcgttcac actccccgat gagggccagc tctgggggaa
     9841 gtgggcgctc cgcctgcgca tcaacgagc
//
LOCUS       DASQDO010000096         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_867013, whole genome shotgun sequence.
ACCESSION   DASQDO010000096 DASQDO010000000
VERSION     DASQDO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867013"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VEV43_02965"
     CDS             complement(<1..971)
                     /locus_tag="VEV43_02965"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYP22510.1"
                     /translation="MAELTYREAVRDAMSEAMRRDEDVFIMGEDIAEMGGSMGVTQGM
                     LEEFGPDRVRNTPISEMAIVGAGIGAAMAGMRPIVEIMYQDFTTLSMEQLVNQAAKHR
                     YMSGGQLKVPLTVRTQGGAGWSPGAQHAQQLEAWFVHVPGLKVVFPSTPEDVRGLLFA
                     SIYDDNPVVFFEHRTLYGIKGTVPDEVEPIEFGQGRVHREGEDVTVVATGRLVHEALA
                     AAEDAEKEGISVEVFDPRTLLPLDEDGVVASVKKTNRCVVAHEAVVRMGFGAEIAALV
                     QERAFDYLDAPVARVGSRFAPLPFAPVMETYVVPHQEDVLAAIKRTVG"
     gene            complement(977..>1539)
                     /locus_tag="VEV43_02970"
     CDS             complement(977..>1539)
                     /locus_tag="VEV43_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYP22511.1"
                     /translation="FRDEPRVAVAFFGDGATNIGLFHESLNLAQLWEVPAVFVCENNH
                     WAESTPRHQHTPLEDLTKRAEAFGMTAMTVDGQDVEAVFNTARDALAHARDGRGPVYL
                     VCETFRLTGHYVGDPQVYRPKEELRELRKTQDPIEKLRATLEVADEEWTELEREIEQV
                     VEGSVEFAKNGTDPDPRDALKNIYAD"
BASE COUNT          210 a          542 c          539 g          248 t
ORIGIN      
        1 ccgaccgtcc gcttgatcgc cgccaggacg tcctcctggt gcggcacgac gtacgtctcc
       61 atcacgggcg cgaaggggag cggcgcgaag cgcgatccca cgcgggcgac gggcgcgtcg
      121 aggtagtcga aggcccgctc ctgcacgagc gccgcgatct cggccccgaa gcccattcgc
      181 acgacggcct cgtgcgcgac gacgcagcgg ttcgtcttct tgaccgaggc gacgacgccg
      241 tcctcgtcga gcgggaggag cgtgcgcgga tcgaagacct cgaccgagat cccctccttc
      301 tccgcgtcct cggccgcggc gagcgcctcg tggacgagcc ggccggtcgc gaccacggtg
      361 acgtcctcgc cctcgcggtg gacgcggccc tgcccgaact cgatcggctc gacctcgtcc
      421 ggcacggtgc ccttgatccc gtagagcgtg cggtgctcga agaagacgac ggggttgtcg
      481 tcgtagatcg aggcgaagag gaggccgcgg acgtcctcgg gcgtcgaggg aaagacgacc
      541 ttcaggccgg ggacgtggac gaaccaggcc tcgagctgct gcgcgtgctg cgcgccgggg
      601 ctccaccccg caccgccctg cgtccgaacg gtgagcggca ccttgagctg gccgccggac
      661 atgtagcggt gcttcgccgc ctggttcacg agctgctcca tcgagagcgt cgtgaagtcc
      721 tggtacatga tctcgacgat cggccgcatc ccggccatcg cggcgccgat gcccgcgccg
      781 acgatcgcca tctcggagat cggcgtgttg cgcacgcggt cgggaccgaa ctcctcgagc
      841 atcccctggg tgacgcccat cgagccgccc atctccgcga tgtcctcgcc catgatgaag
      901 acgtcctcgt cgcggcgcat cgcctcgctc atggcgtcgc gcacggcttc gcggtaggtg
      961 agctcggcca cgtccgtcag tcggcgtaga tgttcttgag cgcgtcgcgc gggtccgggt
     1021 ctgtcccgtt cttcgcgaac tcgacggagc cctcgacgac ctgctcgatc tcgcgctcga
     1081 gctcggtcca ctcctcgtcg gcgacctcga gggtcgcgcg gagcttctcg atcgggtcct
     1141 gcgtcttgcg gagctcgcgg agctcctcct tcgggcggta gacctgcggg tcgccgacgt
     1201 agtggcccgt gagccggaag gtctcgcaga cgaggtagac ggggccgcgg ccgtcgcgcg
     1261 cgtgcgcgag cgcgtcgcgc gccgtgttga acaccgcctc gacgtcctgg ccgtcgacgg
     1321 tcatcgccgt catgccgaag gcctccgcgc gcttcgtgag gtcctcgagc ggcgtgtgct
     1381 ggtggcgcgg cgtcgactcc gcccagtggt tgttctcgca gacgaagacg gcgggcacct
     1441 cccagagctg ggcgaggttc agcgactcgt ggaagagccc gatgttggtc gcgccgtcgc
     1501 cgaagaacgc gaccgcgacg cgcggctcat cacggaact
//
LOCUS       DASQDO010000097         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1111554, whole genome shotgun sequence.
ACCESSION   DASQDO010000097 DASQDO010000000
VERSION     DASQDO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111554"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VEV43_02975"
     CDS             complement(<1..927)
                     /locus_tag="VEV43_02975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HYP22512.1"
                     /translation="MRRLAFALLTSAVLAVTAPAAAAASPATAVVAVNDTGINPYHSA
                     FADDSPRAFRHPSTYLPGFPRGAEALRLTLDEPNYWDALDRDCERVWAKVEPGRLYWV
                     PGTRIAGAISFTPTAEIDCSDPQPSDARILDTAGHGTMTASRAAGKVTGACPACLVVA
                     VEGWGEDFREGVDWITSNAGWIDVQSHSWGLTRPFLEPTGTVSATPEFARSMEKAAAT
                     HPFFRASGNAALLAGQPIHADPQYTPSTIVVGAHDSGSATLWHGFTPQVVADGCGAPA
                     AVSGSTSEIDAAGASGTSSATPWAAGTAARLVL"
     gene            complement(933..1367)
                     /locus_tag="VEV43_02980"
     CDS             complement(933..1367)
                     /locus_tag="VEV43_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22513.1"
                     /translation="MKKNKLVAAAMSALVLGALPAQAARTRARTTTEPYSTPYLGHPE
                     VYGMCMNDMGCTRFGVGTKERFVTVEVSDDAGLDAYGYVVQSDDEGVISSAVICGQST
                     EPIPIEPSIEVRVLLSPVTGTENPCPSVATSGEVTATFSARR"
     gene            1608..1925
                     /gene="mihF"
                     /locus_tag="VEV43_02985"
     CDS             1608..1925
                     /gene="mihF"
                     /locus_tag="VEV43_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HYP22514.1"
                     /translation="MPLPPPLTEEQRKQALEKAAIARRKRAELKEQLKSGRTSLKELL
                     DRTADEVVGKMKVSTVLESLPGVGRVRARKIMERLDISESRRMRGLGAKQKENLLLEF
                     SRR"
     gene            1933..2532
                     /gene="gmk"
                     /locus_tag="VEV43_02990"
     CDS             1933..2532
                     /gene="gmk"
                     /locus_tag="VEV43_02990"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYP22515.1"
                     /translation="MSWRPGGGSSGRLFVVSGPSGAGKGTVVRGVLARRPDVFLSVSA
                     TTRPARQGERHGIEYHFIPEAEFLGMRDRGEFLESAEVYGNYYGTPRGPVESALRVGK
                     DVLCELDIQGAQAIKRAEPHAILIFIEPPSLDDLKVRLRGRGTEDHDTLAQRLRAAYD
                     EVKVKGIYDHIVVNDQVERAVDELLRIMDGSQTSKGTPR"
     gene            2529..2777
                     /gene="rpoZ"
                     /locus_tag="VEV43_02995"
     CDS             2529..2777
                     /gene="rpoZ"
                     /locus_tag="VEV43_02995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HYP22516.1"
                     /translation="MIEPKIETLLTEVDSKYTLVILAAKRSRQINAYFSQLGEGVAEY
                     TPPQVPLSPDQAPKALSIALQEVAGGSVAYERTADGIK"
     gene            2794..>3570
                     /gene="coaBC"
                     /locus_tag="VEV43_03000"
     CDS             2794..>3570
                     /gene="coaBC"
                     /locus_tag="VEV43_03000"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HYP22517.1"
                     /translation="MDAVDLGPAAQGKKVLVCVTGGIAAYKVPFVVRSLAELGADVRV
                     VMTRSAARFVGEQTFAALSGNDVYTELFGAGPDVPHVELARSADLVVVAPATANSLAK
                     MALGLAGDLFGNVMLTVRCPVLVAPAMHTEMWEHDATQGHMATLRARGVHVVGPESGA
                     LSSGDSGPGRMSEPDAIVGAALQLLWRAGELDGRRVLVTAGGTQEPIDPVRFIGNRSS
                     GLMGDLIAEEAQRRGAKVTLVSGPSNLVPPAGVDLIRVKTA"
BASE COUNT          531 a         1229 c         1300 g          510 t
ORIGIN      
        1 gaggacgagc cgggccgcgg tcccggccgc ccatggagtc gccgaggagg tgccggacgc
       61 gccggcggcg tcgatctcgg acgtcgagcc tgagaccgcc gccggggctc cgcaaccgtc
      121 ggccaccacc tggggcgtga acccgtgcca gagtgtcgcc gagccggaat cgtgcgcgcc
      181 gacgacgatc gtcgaggggg tgtactgcgg gtcggcgtgg atcggctggc cggccagcaa
      241 cgcggcgttc ccggaggcgc ggaagaaggg atgggtcgcc gctgccttct ccatggagcg
      301 cgcgaactcg ggcgtcgcgg agacggtccc cgtcggctcg aggaacggac gcgtcagacc
      361 ccaggaatgg gactggacgt cgatccagcc cgcgttggag gtgatccagt cgactccctc
      421 gcggaagtcc tcaccccacc cttcgacggc gacgacgaga caggccgggc acgcgcccgt
      481 gacctttccg gcggcgcgcg acgccgtcat cgtcccgtga ccggcggtgt cgaggatgcg
      541 ggcgtcgctg ggctgggggt ccgagcagtc gatctcggcg gtcggggtga acgagatggc
      601 gccggcgatc cgggtcccgg ggacccagta caggcggccc ggctcgacct tcgcccacac
      661 gcgctcgcag tcgcgatcga gcgcgtccca gtagttcggc tcgtcgagcg tcagccgcag
      721 cgcctcggcg ccccgcggga accccggcag gtacgtcgag gggtggcgga acgcccgggg
      781 cgagtcgtcg gcgaatgccg agtggtaggg gttgatcccc gtgtcgttga cggcgaccac
      841 ggcggtagcc ggagaggctg cggcggctgc gggggccgtc accgcgagca ccgccgacgt
      901 caggagcgcg aaggcgagcc ggcgcatcac gatcacctcc gcgcggagaa cgtcgccgtg
      961 acctcgccgc tcgtggccac gctggggcag gggttctcgg tcccggtcac gggagacagc
     1021 aggacgcgga cctcgatgct cggctcgatc gggatcggct cggtgctctg gccgcagatg
     1081 accgcgctcg agatgacgcc ctcgtcgtcg gactggacga cgtagccgta cgcgtcgagc
     1141 ccggcgtcgt cggagacctc gaccgtgacg aaacgctcct tggtgccgac gccgaaccgt
     1201 gtgcacccca tgtcgttcat gcacatcccg tagacctccg ggtggccgag gtacggcgtc
     1261 gagtacggct cggtggtcgt gcgggcgcgc gttcgcgctg cctgcgcggg cagcgcgccg
     1321 agcacgaggg cggacatggc ggcggcgacg agcttgttct tcttcatgtc agccgggttc
     1381 gcccgtcccc atgcgacgcc tccggccgga cctgtcgggt aattgctcgg ttcgcgacat
     1441 ccggcccgcg ctcgaaaacc ccctctggcc tggacgaacg cgatcacctc gcgttgcggg
     1501 gtcaggggcc tgctgctacc atccgcgccg aagccggtcg tcgctgcggc atcccctcga
     1561 cccggccccg atctttctct ggaaaccttt gaaggaggtc cctcagcatg cccttgccac
     1621 ctccgctgac cgaggagcag cgcaagcagg ccctggagaa ggccgccatc gcccgccgca
     1681 agcgggccga gctgaaggaa cagctcaaga gcggccgcac gagcctgaag gagctcctcg
     1741 accgcaccgc agacgaggtg gtcgggaaga tgaaggtctc gaccgtgctc gagtcgctgc
     1801 cgggcgtcgg cagggtccgg gcgcgcaaga tcatggagcg cctcgacatc tcggagtccc
     1861 ggcgcatgcg cgggctcggc gcgaagcaga aggagaacct cctgctcgag ttctcgcgca
     1921 ggtagccgcc acttgtcctg gcgccccggg ggcggctcct ccggccggtt gttcgtcgtc
     1981 tcggggccgt cgggggcggg caagggaacg gtggtccgcg gtgtcctggc ccggaggccg
     2041 gacgtcttcc tctcggtgtc ggcgacgacg cgccccgcac gtcagggcga gcgccacggc
     2101 atcgaatacc acttcatccc cgaagcggag ttcctgggca tgcgcgaccg cggcgagttc
     2161 ctcgagtccg ccgaggtcta cggcaactac tacggaacgc cgcgcggtcc ggtcgagtcg
     2221 gcgctgaggg tcggcaagga cgtgctgtgc gagctcgaca tccagggggc ccaggcgatc
     2281 aagcgcgccg agccgcacgc gatcctgatc ttcatcgagc cgccgagcct cgacgacctc
     2341 aaggtgcgtc tgcggggccg cgggaccgag gaccacgaca cgctcgcgca gcggctgcgc
     2401 gccgcgtacg acgaggtcaa ggtcaagggg atctacgacc acatcgtcgt gaacgaccag
     2461 gtggagaggg ccgtagacga gctgctccgt ataatggacg gctctcagac atcgaaagga
     2521 accccccgat gatcgaaccg aagatcgaga ccctcctgac cgaggtcgac tcgaagtaca
     2581 cgctcgtgat cctggccgcg aagcgcagcc gtcagatcaa cgcctacttc agccagctcg
     2641 gcgagggcgt cgccgagtac accccgccgc aggtcccgct ctcgccggac caggccccca
     2701 aggcgctgtc gatcgcgctg caggaggtag ccggcggctc cgtcgcctac gagcgcaccg
     2761 ccgacgggat caagtagtcc tctcttagat cccatggacg ccgtggacct gggccccgcc
     2821 gctcagggca agaaggtcct cgtctgcgtc accggcggca tcgccgcgta caaggtcccg
     2881 ttcgtcgtcc ggtccctggc cgagctgggg gccgacgtcc gcgtcgtgat gacgcgttcg
     2941 gccgcgcgat tcgtcggcga gcagacgttc gccgcgctct ccggcaacga cgtctacacg
     3001 gagctgttcg gcgcggggcc ggacgtgccg cacgtcgagc tggcgcgctc cgccgacctc
     3061 gtcgtggtcg cgcccgcgac ggcgaactcg ctggcgaaga tggcgctcgg cctcgccggc
     3121 gacctcttcg ggaacgtgat gctgaccgtg cgttgccccg tgctcgtcgc gccggcgatg
     3181 cacaccgaga tgtgggagca cgacgccacc caggggcaca tggcgactct gcgagcgcgg
     3241 ggcgtgcacg tggtcgggcc ggagtcgggc gcactctcgt cgggcgattc gggtcccggc
     3301 cgcatgtccg agcccgacgc gatcgtgggg gccgcgctgc agcttctgtg gcgagccggc
     3361 gagctcgacg gccgccgcgt gctcgtaacc gccgggggga cgcaggagcc gatcgatccc
     3421 gtgcgattca tcgggaaccg ctcgtcgggg ctgatgggcg acctgatcgc cgaggaggcc
     3481 cagcgccgcg gcgcgaaggt gacgctcgtg tcggggccct cgaacctcgt gccaccggcg
     3541 ggcgtcgacc tgatacgggt gaagacggcg
//
LOCUS       DASQDO010000098         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_316409, whole genome shotgun sequence.
ACCESSION   DASQDO010000098 DASQDO010000000
VERSION     DASQDO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316409"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VEV43_03005"
     CDS             complement(<1..228)
                     /locus_tag="VEV43_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22518.1"
                     /translation="MSARKTTPPSELERLARWTATPAGIATLFVAGFLLRLLLARGGG
                     FPSDIAVFQSWAQRLADVGPGGFYSPDFFADY"
     gene            complement(245..1387)
                     /locus_tag="VEV43_03010"
     CDS             complement(245..1387)
                     /locus_tag="VEV43_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22519.1"
                     /translation="MKAGKKHDRRGIRDLPPSTVILGIVALVVVPRVLLFVTGLAASA
                     EHSGQELDDAATAMWHHWDPGHYLRIADVGYLSSGEDALFIVFFPLFPLLVAGAGLVL
                     RNVTVASLVVTTAASIASGFLLYRLVRLDHPEGQARGAVVLLFAFPTAYALFAPYSEA
                     VFLAAVLGSVYAARTGRWWLAGLAGMAATATRIVGLALVPALLVEAFLAQRGDGRGPR
                     KLLAIGLVPLGFAAYLGINQVVHGDPLHFLQVQEDHWYQRMVAPWTPVTDAIEAMRGA
                     ELTVETFLVHPARLLAIAFAVAVLAVGWRSLRATDRVFSAATVLMVLSASWLISLPRY
                     LLAIYPLFTTMGALGRRRALLWAAAALCFVLQVLLFSRFARGMWAF"
     gene            1326..2096
                     /locus_tag="VEV43_03015"
     CDS             1326..2096
                     /locus_tag="VEV43_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYP22520.1"
                     /translation="MTVDGGRSRIPLLSCFFPAFNEEENVAAVLDEALETLPRFAEDF
                     EVIVVDDGSSDRTAEVVTAYAAGRERVRLVSHEVNRGYGEALRSGLAAAKGDAVFFTD
                     ADRQFRLDDIERLLEHYDESSFVVGYRQKRNDPWHRLVVARVYHAVLKAVFGLRLRDV
                     DCAFKLFSREVLDRIRPDLVSRSAFISPELLIRARMAGVRVTEVGVPHYPRAAGRPKG
                     ATPKVIARTIKEIVVMRRRLGAVGGGDAENAKKLRSEG"
     gene            2185..>2582
                     /locus_tag="VEV43_03020"
     CDS             2185..>2582
                     /locus_tag="VEV43_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22521.1"
                     /translation="MKVKLLIAFVAFLLVVAGLSGYLAIAVGGELYEARSALSGSVAD
                     LDREALRNARRHLEAAGEKLDGFAADVLSIVPIAGHGTRAVERVTEATIGVVDAADSL
                     TYRLEALEALEIVEGDQIRLDLVRQLRAPFT"
BASE COUNT          424 a          907 c          924 g          327 t
ORIGIN      
        1 gtagtcggcg aagaagtcgg gcgagtagaa gcccccgggg ccgacgtccg cgaggcgctg
       61 cgcccagctc tggaacacgg cgatgtcgct ggggaagccc ccgccgcgcg cgagcaggag
      121 cctcagcagg aaccctgcga cgaacaacgt cgcgatcccc gccggcgtcg ccgtccagcg
      181 cgcgaggcgc tccagctcgc tcgggggcgt cgtcttccgg gcgctcatgc gggcaaacga
      241 taggtcagaa cgcccacatc ccccgggcga agcgcgagaa cagcagcacc tgcagcacga
      301 agcacagcgc ggccgcagcc cacagcaggg cgcgccggcg cccgagcgct cccatcgtcg
      361 tgaacagcgg gtagatcgcg agcaggtacc ggggcaggct gatcagccac gacgccgaca
      421 gcaccatcag caccgtggcg gcggagaaga cgcggtcggt ggcgcgcagc gaacgccagc
      481 ccacggcgag gacggcgact gcgaaggcga tcgcgagcag ccgggcggga tggacgagga
      541 acgtctccac ggtgagctcg gccccccgca tcgcctcgat cgcgtcggtc accggggtcc
      601 acggcgcgac catgcgctgg taccagtggt cctcctgtac ctggaggaag tgcagcgggt
      661 cgccgtggac cacctggttg atcccgaggt aggcggcgaa gccgagcggg acgagaccga
      721 tcgcgagcag cttgcggggg ccgcgtccgt cgccgcgctg ggcgaggaac gcctccacca
      781 gcagcgcggg cacgagcgcc agcccgacga tccgcgtcgc ggtggcggcc atccccgcga
      841 gccccgccag ccaccaccgg ccggtccgcg cggcgtagac cgacccgagc accgccgcga
      901 ggaaaacggc ctcggagtac ggcgcgaaga gcgcgtacgc ggtcgggaac gcgaacagca
      961 ggacgaccgc gccgcgggcc tggccctcgg ggtggtccag ccgcacgagc cggtacagca
     1021 ggaaccccga cgcgatcgag gcagcggtcg tgacgacgag cgacgccacg gtgacgtttc
     1081 gcaggacgag cccggccccc gccacgagta aagggaacag ggggaagaag acgatgaata
     1141 gagcgtcctc gcccgaggac aggtacccga cgtcggctat gcggaggtag tggcccgggt
     1201 cccagtggtg ccacatcgcg gtcgccgcgt cgtcgagctc ctgcccggag tgctccgccg
     1261 acgccgcgag gcccgtgacg aacagaagga cccgtgggac gacgaccagt gcaacaatgc
     1321 cgaggatgac cgtagacggc ggcaggtcac gtatccctct cctgtcgtgt ttcttccccg
     1381 ccttcaacga ggaggagaac gtcgcggcgg tcctcgacga ggccctcgag accctgcccc
     1441 ggttcgcgga ggacttcgag gtcatcgtgg tggacgacgg gtcaagcgac cgcaccgccg
     1501 aggtcgtcac cgcgtacgcc gcgggccggg agcgtgtccg tctggtcagc catgaggtga
     1561 accgaggata cggggaagcc ctgcgctcgg gcctcgccgc cgcgaagggc gacgcggtct
     1621 tcttcaccga cgccgacagg cagttcaggc tcgacgacat agagcgcctg ctcgagcact
     1681 acgacgagtc ctcgttcgtc gtcggctacc ggcagaagcg caacgacccc tggcatcgtc
     1741 tcgtggtcgc gcgggtgtac cacgccgttt tgaaggcggt cttcgggctg cggctgcgcg
     1801 acgtcgattg cgctttcaag ctcttctccc gcgaggtcct cgaccggatt cggcccgacc
     1861 tcgtgtcgcg ctcggccttc atctcccccg agctgctcat cagggcccgg atggccggcg
     1921 tacgggtgac cgaggtgggg gtcccccact acccgcgcgc ggcgggccgg ccgaagggcg
     1981 cgaccccgaa ggtcatagcc cggacgatca aggagatcgt cgtgatgagg aggcgcctcg
     2041 gcgccgtcgg gggcggcgac gcagagaacg cgaagaaact tcgatccgag ggctaaagga
     2101 gcggccccga gccaccgata tcactgggtt gggtcgtctc gccccggcca ctcccccgcc
     2161 cgggcccgct cgtagagtgt cgccgtgaag gtcaagctcc tcatcgcctt cgtcgccttc
     2221 ctcctcgtgg tcgccgggct ctcggggtac ctcgcgatcg ccgtcggcgg cgagctctac
     2281 gaggcgcgct ccgcgctgtc cggctccgtc gccgacctcg accgcgaggc tctgcggaac
     2341 gcccgccgcc acctcgaggc cgccggcgag aagctcgacg ggttcgcagc cgacgtgctc
     2401 tcgatcgtcc cgatcgcggg gcacgggacg cgtgcggtgg agcgggtgac cgaggcgacc
     2461 atcggcgtcg tcgacgccgc ggactcgctg acgtaccggc tcgaggcgct ggaggcgctc
     2521 gagatcgtcg agggcgacca gatcaggctc gacctcgtcc ggcagctccg ggcccccttc
     2581 ac
//
LOCUS       DASQDO010000099        22067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_989875, whole genome shotgun sequence.
ACCESSION   DASQDO010000099 DASQDO010000000
VERSION     DASQDO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989875"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VEV43_03025"
     CDS             <1..254
                     /locus_tag="VEV43_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22522.1"
                     /translation="IELFPELASADSPLTSWEAEVGFFTKWEPPWAVLLGQVGFFDKF
                     TVTMSRLAQALAVERKEVFDQRFGHIYRTADDRAPRFEP"
     gene            329..2449
                     /locus_tag="VEV43_03030"
     CDS             329..2449
                     /locus_tag="VEV43_03030"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HYP22523.1"
                     /translation="MSSRARFLIPSVVAALLAGPVVPPAAAQEPLYTITLVTQRFWHG
                     ADDPLDVGIKVTNLSEAPLEGFRLQISSYERVTSRSALEESFDGDPFGSPTAVTFEQF
                     DAVVPAGDSKVVAVRTPVSELSTLALGSGGVYPLGIDLADPNGIPLGASVVTQLVYYP
                     GEPLETQLSTVLVVPLNDLAARGPDGVFEETENGVVPLEEALAGDGWMRGTLDAMTEA
                     AGAGLHFALAPTPRLVEEIADMADGYTRGASDGAEEQVPRDAPAAQNAAAALDALTKL
                     LDTNGVQPLLVPYSFADLPALAHTDAATPGPIARQLTVAEEVLGALELRKSGFTRRWV
                     FPPAGRLDGRALTELRALSAETGTRTFFSAQSLVPPVNPDAAGCPESFASFACPVAVA
                     GTVGYEADAGLQQRFIALAQPGEDRLDLQRLLAETAMIREELPGRTDRVIQATMPSLW
                     HPRPKLAGLMFRAFARAPWLSTLTPNQGLDQSAAPVERQPVSVIGDLPRTPDESYFDR
                     LADAEQVVDDVREIGAPEALVTRLERDILVAYGRTWWSDETLAEVGAAFAEEARLEAE
                     RHLGRIAIRAAPEITMTSRSTDVQFLVTNDNDYPVRLDVALESPNLVVDDLDLPAEFP
                     PRATTPVQASVAAQTSGVFQVELTATTPSGSVVDELVISVRSTELNQIALGITLGALA
                     FLISFYAYRAVRRRRAREEQAAAT"
     gene            2446..4032
                     /gene="murJ"
                     /locus_tag="VEV43_03035"
     CDS             2446..4032
                     /gene="murJ"
                     /locus_tag="VEV43_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYP22524.1"
                     /translation="MRGAGARATALMTLGTVFSRITGVARLAAIVAALGVAETRLTDT
                     YNLANTFPTIVYELILGGVLTSVFVPVFVELLEKEGRDRAWEVASGILNLALVVLSAM
                     TIAGIVAAPWIAKLYALRLEGDQVEHQREALTFLLRLFLPQMVFFALTAITAGLLNAH
                     RRFGAPMYTPILNNLAVVAVFVTFYQLYGRVTLETVSTSQLLLIGLGTTAGVVLMALA
                     QLPFLRGLGRYRLTLQFRHPAFRKLWRLSLFVIAYVVVNQIGYFVVQILANEQQGGYT
                     AYVNAFIFFMLPHGLFAVSIVTALLPGMSALAVDRDWAGFAERFSTGVRATVFLVLPA
                     AVGYFVLGEPIVRLLVERGVVTERSTELIAGVLRVFVVGLVPFSLFQLSLRAFYALQD
                     TKTPFLVNCGAVALTTGLNVPLFHVYGVRGLAAGHACGYVAGAFAQERLLARRLGRAG
                     FLAGTARTVAAAVAMGAVVWLALRGVRAVTAEGTFAATLSEVVVPVAAGAISYLAFAR
                     LLRVPELDHVAALVRRRKAS"
     gene            4062..4484
                     /locus_tag="VEV43_03040"
     CDS             4062..4484
                     /locus_tag="VEV43_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22525.1"
                     /translation="MDLRAFGRDESGIVFAWLARLVVGLALMGVVLFDGGSIVVNYFS
                     LDSATDEVALEVVTRMGSGAEIIPNRDCTRRAVDPTCRAVYDVAREKGVRIVSARFDA
                     EGVFHVETRRTADTLIVGRIGAIEDWATATASARADTN"
     gene            4495..6021
                     /locus_tag="VEV43_03045"
     CDS             4495..6021
                     /locus_tag="VEV43_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYP22526.1"
                     /translation="MASDRRDVPGAAPAITIADRYVLEELLARGGMGSVYKARDEVLA
                     RPVAVKILHPHLSEDPAFLERFRREAVAAAGLGHPGIVAIYDTGTDATDEGERHYIVM
                     EHCAGGTLTDLVAAERAFDPGRACGIAAAICSSLDFAHRAGVVHRDVKPDNVLFSDDG
                     TLKVADFGIAKAAYGSGDITTTGSILGTVTYISPEQAAGGEPDARSDVYSTGVVLYEL
                     LAGRPPFKAETDVATAMMHLKDQPTPLRALKPHVPRHLDATVTKALAKDPEQRWSSAD
                     EMRRALEGTPSGGTTAVIARPPSLERETTPAEPHRSDTRWVGPVLALIVFGLLLAFLV
                     PRLLEDEPSGGGRSRPDSRSGPARGGAGAPLEVAEAGSLDPDGDGDEHSDEAPLAIDG
                     NPSTAWGAEDYQDSLELIGKSGIGLVLDLGSAQDVTSVEIATSTPGFTFEIRAGDSAP
                     QVAADLEEVGSVDAAGASEEVTFDEEVGARYWLVWITKLPGEGGGTVEIQEVTLRGSS
                     "
     gene            6008..6589
                     /locus_tag="VEV43_03050"
     CDS             6008..6589
                     /locus_tag="VEV43_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP22527.1"
                     /translation="MEAPSRREALTDEDLLKQAAAGDQSAFSALVRRHEDRVFGIAVR
                     IMGDRADALDATQDTFVSVFRQAGSFRAEAAFTTWLYRVAVNACRDLLRKRRRLPEPT
                     DELPERPRPGIGTEEVVGLRLDLARALAQLPDEYRDAVLMHDLGAIPYEEIAQITGVA
                     LGTVKSRISRGRRRLAELLEQPAPTSASKDQDG"
     gene            6594..7535
                     /locus_tag="VEV43_03055"
     CDS             6594..7535
                     /locus_tag="VEV43_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22528.1"
                     /translation="MTHDRCSELLRGYARGELPDDVSDEVRAHLATGGDCRAEERAVA
                     ALAVTDGVDARPLDDVERARLQRGLAQELFTARANADVAGAAAPERRWARWIAPAATA
                     AAVLAGVLVMTLGGGADDAAETALQPFSRAGEGGADAGGADDSAAQSQAGGQVEDKRG
                     RRRGLTAATGQASTESDFAGPEQPAPVFDPDGGTITTSELSEIGRGRLFRSFADSYRP
                     EDGPGLYDRFLEQLTTDAVALGPQIEACAATLPQDGSLIPTYATVGEYDGRRALVVGF
                     VTGDPGSRTLDRYLMWVWEIDVCEQPIDTLFERIDSG"
     gene            7585..8523
                     /gene="trxB"
                     /locus_tag="VEV43_03060"
     CDS             7585..8523
                     /gene="trxB"
                     /locus_tag="VEV43_03060"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HYP22529.1"
                     /translation="MPEQRRVIIIGSGPAGLTAAVYTSRASLEPLVIEGIEAGGQLML
                     TTEVENYPGFVDGIMGPELMEAMRKQAARFGTEFLTDNVTSVDFSRKPFVVETASTTY
                     EAGAVIISTGASARMLGVPGERELLGHGVSTCATCDGFFFRGQEIVVVGGGDSAMEEA
                     NFLTKFASKVTVVHRRESLRASKIMQDRARANTKIEFVFDSVVKQINGNGKVASATLL
                     NVKTGAETDLPAAGVFVAIGHTPNTSLFEGQLDLSGGYIVTHDGTATSVEGVFAAGDV
                     VDFRYRQAITAAGMGCMAAIDAERFLEDVPITERFW"
     gene            8558..9124
                     /locus_tag="VEV43_03065"
     CDS             8558..9124
                     /locus_tag="VEV43_03065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYP22530.1"
                     /translation="MRSLLSRALPAVLLVAVSAPAPRAQPAPRMVFPIVRSGWYSVIN
                     FTDDWHDPRMRRTADGWQLIGRHEGTDVFAEPGTPVVAILGGRVEQLGWTFYSGWRVG
                     VRGDDGRYWFYAHLSSFAPGLSIGSPVAAGQQLGEVGNTGYGATPGHRDEFTYHLHLG
                     IQEPDGTWVNPYPLVRRLYDAAVRRDGA"
     gene            9121..10197
                     /locus_tag="VEV43_03070"
     CDS             9121..10197
                     /locus_tag="VEV43_03070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYP22531.1"
                     /translation="MTSGPARRDAGIEGLIRQGDRSQQVADVQARLRALGLAVDDEAG
                     VFGDSTRHAVRTFQQTRGVLVDGIVGPNTWTELVEASWRLGDRSLYLKVPPFRGDDVS
                     LLQKRLNALGFDSGREDGIFGEDTARGVKEFQKEYGVAEDGIWGPRSQSALAGLRVDR
                     PGTTAQLREELRRSPDLHGALVVLDPGHGGSDPGARAAAGLLEADVAWDLAVRLADKL
                     SGMGARVRFTRTEAEAPDASERARRANEVAGDVFLSLHLNEHEEPTAEGASTYYFHTS
                     AAGRSLAESIQEGLVALGVRDCRSHACSYTVLRETRMPAVLVEPVFVSNPDEAKRLEE
                     ADFRAALADAIATGVSRYYASTPV"
     gene            complement(10194..11087)
                     /locus_tag="VEV43_03075"
     CDS             complement(10194..11087)
                     /locus_tag="VEV43_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005446403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYP22532.1"
                     /translation="MTRKSGLGRGLDALLPSGGGGEAPEARGNLLELPLDAIVANPHQ
                     PRRTFDEAGIAELATSIGELGILQPLLVRDLGGGTYELIAGERRLRAAHAAGLKTVPA
                     IAVETDERGSLERAIVENIHRADLNPIEEAAAYRQLIAEAGLTQEELGDRLGKNRVTI
                     TNALRLLDLPTSLQRLLIEGKLSAGHGRALLRLSDNPFQERMARRAAEEKLSVRETAD
                     EVARYLAMTGGVTAGRAGSRRAAEESGAVAEAQRRLGDHLQTRVKVTMGARKGKITLD
                     FVSPDDLARIVAAITGRPPSD"
     gene            complement(11084..12079)
                     /locus_tag="VEV43_03080"
     CDS             complement(11084..12079)
                     /locus_tag="VEV43_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP22533.1"
                     /translation="MTDNSSKNWKLLERRPIQGGRGRIVCVANQKGGVAKTTTSVNLA
                     AGLALRGHRVLVVDVDPQSNAATGLGIDHRNVERSTYHLLLGDATLDDVIRPTAIKNL
                     DCAPASLDLAGAEVELVSTMARERRLGEALAGAAERYELIFLDCPPSLGLLTVNALAA
                     AQDLFVPVQCEYYALEGLGQLLGNAERVRRSLNPDLRIAGFLLTMYDGRTKLSSQVEN
                     DVRSHFGELVFETRIPRSVRLSEAPSFGEPVLTLDPSSRGAVAYRLLAAEVETLYGLE
                     VHKPPPPPPAPAPVLTASRPSTPGPGGRGYGVKTPRPDLPDSVWPRGEPFTVPGP"
     gene            complement(12076..12609)
                     /gene="rsmG"
                     /locus_tag="VEV43_03085"
     CDS             complement(12076..12609)
                     /gene="rsmG"
                     /locus_tag="VEV43_03085"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HYP22534.1"
                     /translation="MDPNELLRRYAALVREWAPRLDLVSPRDLERFEERHVEDSLRAA
                     PLLDELPEGPCVDVGSGAGLPGIPLSIARPERHWTLLEPRQRRAAFLEEAVRRLGLDC
                     EIVAATAEQAARDPRLAGAHVLVTARAVADPAAVRELAEPLLSPGGVTLVFTGPDAAA
                     PFDADEWEPGLTIMRKK"
     gene            complement(12688..13371)
                     /locus_tag="VEV43_03090"
     CDS             complement(12688..13371)
                     /locus_tag="VEV43_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22535.1"
                     /translation="MRARKLAVTALAMGLVVGSLGVAEAAKKKKKPKPAAPVAVDQTF
                     YMRRDACTGAGADSADNARLSLVDGVDEGNLCGWGESGLLNEAVIASGQLDAVADVWP
                     VADGLPLTVDATKPISGKLQMVSSFLGAAPDNGLAGGNVEVVIDFTGVSGGEEVVVGT
                     ATASYQVTPASSRYEVPFEIKLDGALDKKSFEALTMTTTIRGANFGHGLYELDQPAST
                     VVIPSWVVR"
     gene            complement(13507..14064)
                     /locus_tag="VEV43_03095"
     CDS             complement(13507..14064)
                     /locus_tag="VEV43_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HYP22536.1"
                     /translation="MTEEREESYADAELEQPLEGDPPAESGAEEELEPATPEDVASTA
                     EEFVSGLLKSLELEGEVSSSVEGDTATVEVSGEALGGLIGRRGQTLDALQEITRTAVQ
                     RRLRGRVRVLVDVEGYRARRRASLAEYARSIAERAKERGTEIELEPMTAYERKVVHDA
                     VAEIDGATSFSEGVEPNRKVVVRGE"
     gene            complement(14061..14999)
                     /locus_tag="VEV43_03100"
     CDS             complement(14061..14999)
                     /locus_tag="VEV43_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HYP22537.1"
                     /translation="MGFFELFSTALAGFFALIPSYGLAIILLTLAVRLLLLPLSIKQT
                     KSMREMQAIQPEIKRLQAKHKGDRQKMNEELMALYKEHGVNPFGGCFPLLMQFPVLIG
                     LFYVIRDPLKYMGDVVENSALADGLRDIPVQINSFLGIHLNCSANGTLTGQHDVGEGT
                     AALFEACGSGFVTALPYLGLVLFMGFTTYYQQKQMQRSTPDSPQAQQMQMITKVMPLL
                     LMVFSFNFPAGVVIYWVTTNLWTIVQQRIILAAHPPVVLGAGGGATLPKKGAGKTPAT
                     TKATTANASKASPNGGKRPEGAASKPHPSSKKKRKK"
     gene            complement(15000..15272)
                     /gene="yidD"
                     /locus_tag="VEV43_03105"
     CDS             complement(15000..15272)
                     /gene="yidD"
                     /locus_tag="VEV43_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYP22538.1"
                     /translation="MSPLGRVLVALIGFYRRAISPLLGPHCRFAPTCSAYAAEAIQTH
                     GAFRGGWMAVKRVARCHPWSAGGVDHVPPARGRAGRLGGTPEGEPS"
     gene            complement(15269..15604)
                     /gene="rnpA"
                     /locus_tag="VEV43_03110"
     CDS             complement(15269..15604)
                     /gene="rnpA"
                     /locus_tag="VEV43_03110"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYP22539.1"
                     /translation="MPSIEPLSGKPAFARVHATGRRCTKNGVTAVVSVRDEGPARLGL
                     SVGKSAGKAVVRNKIRRRLREAFRAYGPGSADVVLVGRGQVATEPFSRVEEYVRQSLS
                     GAGVPRRPS"
     gene            complement(15626..15763)
                     /gene="rpmH"
                     /locus_tag="VEV43_03115"
     CDS             complement(15626..15763)
                     /gene="rpmH"
                     /locus_tag="VEV43_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYP22540.1"
                     /translation="MTKRTFQPNTRRRKRKHGFRARMKTRAGRAILKARRAKGRERLS
                     A"
     gene            16224..17654
                     /gene="dnaA"
                     /locus_tag="VEV43_03120"
     CDS             16224..17654
                     /gene="dnaA"
                     /locus_tag="VEV43_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYP22541.1"
                     /translation="MTGRAWMQSSISEDSPAPATSSTGDTAAAIWERATEDLKLALSE
                     SNFSAWFGRTQAVGLDGDTLVLGVPNHFTKEWIEGRFLEPLVAAVTTAAGRSLEVAIL
                     SDAAYATGDPAPQGPAPEVITLAESPEVPADDVFHPNYTFDSFVIGSSNRFAHAAALA
                     VAEAPAQAYNPLFIYGGAGLGKTHLLHAIGRYAHQWQPNTVVRYVSTEQFMNDFILAL
                     QRRNIPAFHRRYRMADLLLIDDIQFLEGKERTQEEFFHTFNALHPKSQIVITSDRPPK
                     KISTLEERLRTRFEWGLITDIQPPDLETRLAILQKKAETEQLVVGHEVMSFIASRIQT
                     NIRELEGALIRVAAYASLTRSDLTLDLAQQVLRTLLPESREARVTSDLVVSVAAEYYD
                     VTPEEICSSSRSRPLVNARQMAMYLCRELTDLSLPKIGERFGGRDHSTVLYAMHKVRQ
                     HMKERQSCYQQVEELTTRIRQQAARG"
     gene            17925..19064
                     /gene="dnaN"
                     /locus_tag="VEV43_03125"
     CDS             17925..19064
                     /gene="dnaN"
                     /locus_tag="VEV43_03125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYP22542.1"
                     /translation="MKFRCERDDLLEAVQFASRAISNRATLPVLSGLRIDATEEGAVT
                     VAATDLELTMETSFQAGVDEPGKTIVPGRLFGEMSRSLSSGAVTVGGGEGEVEIGSGR
                     GNFRVKSLAPDDYPALPIEGREDAGDGVVVDVDGNLLATALSQVVRSASGDESRQVLT
                     GVLWELESGSLTLAATDSYRLGVRTLKVDGGPAELRKVILPARALHELARALQGGGSS
                     VKATVKDNLVAFAIPAAGEGSGGGESVIGSRFIEGEFPNYRQLIPQGYPNALTVEREA
                     LLEVTKRVGLLAQNNLPVKLQLGAELEVSAHTPDVGEGQEVIDAEYQGEPLVIAFNPQ
                     FLHDGASAIQSEKIVIEVSDGLKPAILRGESDEAFTYLLMPVRLS"
     gene            19089..20201
                     /locus_tag="VEV43_03130"
     CDS             19089..20201
                     /locus_tag="VEV43_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HYP22543.1"
                     /translation="MRVDALALEDWRNYERQSVDLSPGLNLVVGANAQGKTNLLEAAY
                     CLSGFGSPRAADAALVRHGCERALLHASVERRGRTVRIDLEVRPGRGSRALLNKTPVQ
                     PRLLGDVCVSVFFGPDELSLVKGSPDGRRRVLDELIVKLRPARDGIRREFERTLKQRN
                     ALLKSAPRGGSATVSSTLDVWDAALARAGAALAAARLEALTGLIPLAAKRYAEVAGAG
                     TLTLEYASSWVGENTAPDAASLEEQLRAAIAEARPREIERGVGLVGPQRDDVSVILDG
                     VDARVFASQGDQRTCALALKLGEHDLLSDALGEEPVLLLDDVFSELDPSRRAWLADAV
                     RSIGQTLVSTAEPVALDALSPGATFSVAGGTVVRSA"
     gene            20194..20622
                     /locus_tag="VEV43_03135"
     CDS             20194..20622
                     /locus_tag="VEV43_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HYP22544.1"
                     /translation="MRDEPLRLKDLLPDATGRLGIPSPEATGRVWSAWKDIVGPAVAA
                     HAEPTSLRGGVLRVRADSPAWATEIGYLVDEIRSRANAVTGRPLVSEVRVWTGPGSTL
                     PAGAGATVEPASEEPERERPQDPAEALERARRAWARRRRR"
     gene            20800..>22067
                     /locus_tag="VEV43_03140"
     CDS             20800..>22067
                     /locus_tag="VEV43_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP22545.1"
                     /translation="MTVASDAREPAKKRRDGSYTGQDITVLRGLDPVRKRPGMYIGST
                     GIRGLHHLVYEVVDNAVDEAMAGYCDRIVVELLADGGCRVSDNGRGIPVDPIETDGRK
                     KLPAATVVLTTLHAGGKFEGKGYQVSGGLHGVGVSVVNALSERLELEIVREGSLHRQE
                     FERGTPLGPLTKVKPAKRTGTTVGFWPDPLIFTEEREFKQETLAQRLREMAFLNRGLE
                     IRLVDRRVEPATEETFRYTGGIVDFVKHLNSSRDAVNKRPVFFDAKAEGHEVEVAMQW
                     TTSFNESVFTFANNINTHEGGMHEEGFRRALTRVISKYARDKNILKEKDDAFTGEDCR
                     EGLTAIVSVKLRDPQFEGQTKTKLGNTEIRSFVETSMNQRFAEFMEEHPSEARAICNK
                     ALSAQRARLEARKARDLVRRKSLLESSTLPG"
BASE COUNT         3221 a         7748 c         7785 g         3313 t
ORIGIN      
        1 cgatcgagct gtttccagag ctcgcctcgg cggattctcc gcttacttcc tgggaagcag
       61 aagtcggatt cttcaccaag tgggaaccgc cttgggcggt cttgcttgga caggtcggat
      121 ttttcgacaa gttcacggtg acgatgagtc gactggcgca ggcgctcgcg gtggagcgga
      181 aggaggtctt cgatcagcgt ttcggtcata tctaccgaac cgctgacgac agagcgcccc
      241 gattcgaacc gtagttgagt cagcgcgtgc tagcgcgtgc aggggtaggc cccggccccc
      301 tagacgccgc ggtccttatg ctggctccat gtccagccgg gcccgattcc tgatcccgag
      361 cgtcgtcgcc gcgctccttg ccgggcccgt ggtgcctccc gccgctgcgc aggagccgct
      421 ctacacgatc acgctcgtca cgcagaggtt ctggcacggc gcggacgacc cgctcgacgt
      481 cgggatcaag gtcacgaacc tctccgaagc tccgctcgag ggcttccgcc tccagatctc
      541 gtcctacgag cgagtcacca gccggtcggc cctggaggag agcttcgacg gcgacccctt
      601 cggttcgccc accgccgtca cgttcgagca gttcgacgcc gtggtcccgg cgggcgactc
      661 gaaggtcgtg gccgtccgca cgccggtgtc cgagctctcg acgctcgcgc tcgggagcgg
      721 cggcgtctat ccgctcggca tcgacctcgc cgacccgaac gggatcccgc tcggggcgtc
      781 ggtcgtgacc cagctcgtct actaccccgg cgagccgctg gagacccagc tgtcgaccgt
      841 gctcgtcgtc ccgctgaacg acctcgcggc acgggggccg gacggcgtct tcgaggagac
      901 cgaaaacgga gtggtcccgc tcgaggaggc gctcgcgggg gacgggtgga tgcgcgggac
      961 gctcgacgcg atgaccgagg cggcaggggc gggcctccac ttcgcgctcg ctccgacgcc
     1021 gcgcctcgtc gaggagatcg cggacatggc cgacggctac acccgcggcg catccgacgg
     1081 ggccgaggag caggtcccgc gtgacgcccc cgccgcgcag aacgccgcgg cggccctcga
     1141 cgcgctgacg aagctgctcg acacgaacgg cgtccagcct ttgctcgtcc cgtactcgtt
     1201 cgccgacctc cccgcgctcg cgcacaccga cgccgcgacg cccggcccca tcgcgcgcca
     1261 gctcaccgtg gcggaggagg tcctcggggc cctcgagctg cggaagtcgg ggttcacgag
     1321 gcgctgggtc ttcccaccgg cggggcgtct ggacggccgc gcgctcaccg agctccgcgc
     1381 gctcagcgcc gagaccggga cacggacgtt cttctccgcg cagtcgttgg tgccgccggt
     1441 gaaccccgac gcggccggat gccccgagtc gttcgcgtcc ttcgcctgcc ccgtggcggt
     1501 cgccggcacc gtcgggtacg aggccgacgc cgggctccag caacgtttca tcgcgctcgc
     1561 ccagccgggg gaggaccggc tcgacctcca gcggctgctc gccgagaccg cgatgatccg
     1621 cgaggagctg ccgggccgca ccgaccgcgt catccaggcg acgatgccgt cgctgtggca
     1681 cccgcgcccc aagctcgcgg ggctcatgtt ccgagcgttc gcccgggcgc cgtggctgtc
     1741 gacgctgacg ccgaaccagg gtctcgacca gtcggcggcc cccgtggagc ggcagcccgt
     1801 cagcgtgatc ggggacctcc cgcgcacgcc cgacgagtcc tacttcgacc ggctcgcaga
     1861 cgcggagcag gtcgtcgacg acgtgcgcga gatcggcgcg cccgaagcgc tcgtgacgcg
     1921 gctcgagcgc gacatcctcg tggcgtacgg acggacgtgg tggagcgacg agacgctcgc
     1981 cgaggtcgga gccgcgttcg cggaagaggc gcggctggag gccgagaggc acctcggtcg
     2041 catcgcgata cgcgcggccc ccgagatcac gatgacgtcc cggtccacgg acgtgcagtt
     2101 cctcgtcacg aacgacaacg actacccggt gaggctcgac gtcgccctcg agtcaccgaa
     2161 cctcgtcgtc gacgacctcg acctccccgc cgagttcccg ccgcgcgcga cgacacccgt
     2221 ccaggcgtcg gtcgcggcac agacgagcgg cgtgttccag gtggagctca cggcgacgac
     2281 gccgagcggc tccgtcgtcg acgagctcgt gatctcggtc cggtcgacgg agctcaacca
     2341 gatcgcgctc ggcatcacgc tcggcgcgct cgcgttcctg atctcgttct acgcgtaccg
     2401 ggcggtccgg cgccgccgcg cgcgcgagga acaagcggcg gcgacttgag aggtgcgggc
     2461 gcgcgggcca ccgcgctcat gaccctcggc accgtcttct cccgcatcac gggcgttgcg
     2521 cgcctcgccg cgatcgtcgc cgcgctcggg gtcgccgaga cgaggctcac cgacacctac
     2581 aacctcgcca acaccttccc gacgatcgtc tacgagctga tccttggcgg cgtgttgacg
     2641 tcggtgttcg tgccggtgtt cgtggagctg ctcgagaaag agggccgcga cagggcgtgg
     2701 gaggtcgcaa gcggcatcct caacctcgcg ctcgtagttc tctccgcgat gaccatcgcc
     2761 gggatcgtcg cggccccctg gatcgcgaag ctgtacgcac tgcggctcga aggcgaccag
     2821 gtagagcacc agcgcgaggc cttgacgttc ctcctgcggc tgttcctgcc gcagatggtg
     2881 ttcttcgcgc tgacggcgat caccgcgggg ctgctcaacg cgcaccggcg cttcggcgcc
     2941 ccgatgtaca cgccgatact caacaacctc gcggtcgtcg cggtgttcgt gaccttctac
     3001 cagctgtacg ggagggtgac gctcgagacg gtctcgacgt cgcagctgct gttgatcggg
     3061 ctcgggacga cggccggcgt cgtgctgatg gcgctcgcgc agctgccgtt cctgcgcggg
     3121 ctcgggcgct accggctcac gctccagttc aggcatccgg cgttccgcaa gctgtggcgg
     3181 ctctcgctgt tcgtgatcgc ctacgtcgtc gtgaaccaga tcggctactt cgtcgtgcag
     3241 atcctcgcga acgagcagca gggcggctac accgcgtacg tcaacgcgtt catcttcttc
     3301 atgctcccgc acgggctgtt cgcggtgtcg atcgtcaccg cgctgctgcc ggggatgagc
     3361 gcgctcgccg tcgaccgcga ctgggcaggg ttcgcggagc gcttctcgac cggggtccgc
     3421 gcgacggttt tcctggtcct ccccgccgcc gtcggctact tcgtcctggg cgagccgatc
     3481 gtgcggcttc tcgtcgagcg cggcgtcgtc accgagcgct cgaccgagct gatcgccggc
     3541 gtgctgcgcg tgttcgtcgt cggcctcgtc ccgttctcgc tgttccagct ctcgctgcgt
     3601 gcgttctacg cgctgcagga cacgaagacg ccgttcctcg tcaactgcgg cgcggtcgcg
     3661 ctcaccaccg ggctcaacgt cccgctcttc cacgtctacg gcgtgcgcgg gctcgccgcc
     3721 ggtcacgcgt gcggctacgt cgccggcgcg ttcgcgcaag agcgtctgct cgcgcggaga
     3781 ctgggccggg ccgggttcct cgcggggacc gcgcgcacgg tcgcggccgc ggtcgcgatg
     3841 ggggccgtcg tctggctcgc gctccgcggc gtcagggcgg tgacggccga ggggaccttc
     3901 gcggccaccc tgtcggaggt agtcgtcccg gtcgccgcgg gcgcgatctc ctatctcgcg
     3961 ttcgcccggc tgctacgcgt gcccgagctg gaccacgtcg ccgcgctcgt gcggcgccgt
     4021 aaagcctcat gagaagtccg gccgaggccg ataatgaacg cgtggacctc cgagcgttcg
     4081 gccgcgacga gagcggcatc gtcttcgcgt ggctcgcccg cctcgtggtg ggcctggccc
     4141 tcatgggcgt cgtcctgttc gacggggggt cgatcgtcgt gaactacttc tcgctcgaca
     4201 gcgcgaccga cgaggtggcg ctcgaggtcg tgacgcgcat ggggtcgggg gccgagatca
     4261 tcccgaaccg cgactgcacc cgccgggccg tcgatccgac gtgccgcgcg gtgtacgacg
     4321 tcgcccgcga gaagggcgtg cggatcgtgt cggcccgctt cgacgccgag ggagtgttcc
     4381 acgtggaaac gaggcgaacg gcggatacgc tgatcgtggg ccgcataggt gcgatagagg
     4441 actgggcgac ggcgacagcg agcgcacggg cggacacgaa ctagtcttcg ggccatggct
     4501 tcggaccgcc gggacgtgcc cggcgcggcc cccgcgatca ccatcgccga ccgctacgtg
     4561 ctcgaggagc tcctcgcccg cggcgggatg ggctccgtct acaaggcacg cgacgaggtc
     4621 ctggcccggc ccgtcgccgt caagatcctc catccccacc tctccgagga ccctgccttc
     4681 ctcgagcggt tccggcgcga ggccgtcgcc gcggcggggc tcgggcaccc ggggatcgtc
     4741 gcgatctacg acaccggcac cgacgcgacc gacgagggcg agcgtcatta catcgtcatg
     4801 gagcactgcg ccggcgggac gctgaccgac ctcgtcgccg ccgagcgcgc cttcgacccc
     4861 gggcgtgcct gcgggatcgc cgcggcgatc tgctcgtcgc tcgacttcgc gcaccgcgcc
     4921 ggcgtggtcc atcgcgacgt caagccggac aacgtcctgt tctcggacga cggcacgctc
     4981 aaggtggcgg acttcgggat cgcgaaggcc gcgtacggga gcggcgacat cacgacgacc
     5041 ggctcgatcc tcggcactgt gacgtacatc tcgccggagc aggccgccgg cggggagccc
     5101 gacgcgcgct ccgacgtcta ctcgacgggc gtcgtcctct acgagctgct ggccggccgg
     5161 ccgccgttca aggccgagac cgacgtggcc acggcgatga tgcacctgaa ggatcagccg
     5221 acgccgctgc gcgccctcaa gccgcacgtc ccgcgccacc tcgacgcgac cgtgacgaag
     5281 gccctcgcga aggatcccga gcagaggtgg tcgtcggccg acgagatgcg acgcgcgctg
     5341 gagggaacgc cctccggggg cacgaccgcg gtgatcgcgc ggcccccgtc gctcgaacgc
     5401 gagacgaccc cggccgagcc ccaccgcagc gacacccggt gggtcggccc ggtcctcgcc
     5461 ttgatcgtct tcgggctcct cctcgcgttc ctcgtgcccc gcctcctcga agacgagccg
     5521 tcgggcggcg ggcggtcccg gcccgacagt cggtccggcc cggctcgagg cggcgcgggg
     5581 gccccgctcg aggtcgccga agcggggtcg ctcgacccgg acggggacgg cgacgagcac
     5641 tcggacgaag cgccgctggc gatcgacggg aacccctcga ccgcatgggg cgcggaggac
     5701 taccaggatt cgctcgagct catcggcaag agcgggatcg ggctcgtcct cgacctcggc
     5761 tccgcgcagg acgtcacgtc ggtcgagata gccacctcca ccccggggtt caccttcgag
     5821 atccgcgcgg gcgactccgc cccccaggtc gccgccgact tggaggaagt gggctcggtg
     5881 gacgccgcgg gtgcctccga ggaggtgacc ttcgacgagg aggttggggc ccgctactgg
     5941 ctcgtgtgga taacgaagct ccccggcgag ggcgggggga cggtcgagat ccaggaagtc
     6001 acgctccgtg gaagctccta gccggcgcga ggcgctgacc gacgaagacc tgctgaagca
     6061 agcagcagcg ggcgaccagt cggccttctc cgcgctggtc cgccgccacg aggaccgcgt
     6121 gttcgggatc gcggtccgga taatgggcga ccgcgccgac gcgctcgacg cgacgcagga
     6181 cacgttcgtg tcggtgttcc ggcaggcggg ttcgttccga gcggaggccg cgttcacgac
     6241 gtggctctac cgggtggcgg tgaacgcgtg ccgcgacctg ctgcggaaga ggcggcgtct
     6301 gcccgagccg accgacgagc tgcccgagcg tccgcgtccc gggatcggca ccgaagaggt
     6361 cgtggggctg cggctcgacc tcgcccgcgc actcgctcag ctgccggacg agtaccgcga
     6421 cgccgtcctg atgcacgacc tcggcgccat cccgtacgag gagatcgcgc agatcaccgg
     6481 cgtcgcgctg gggacggtca agtcgaggat cagccgcggg cggaggcggc tcgcggagct
     6541 cttggaacaa cccgcgccga cctcggcgtc caaggaccag gatggatgag gccatgacac
     6601 acgacaggtg ctcggagctg ctccgcgggt acgcccgcgg ggagctgccg gacgacgtct
     6661 ccgacgaggt gcgcgcgcac ctcgcgaccg gtggcgactg ccgggccgag gagcgggcgg
     6721 tcgccgctct cgcggtcacc gacggggtcg acgctcgccc cctggacgac gtcgaacgcg
     6781 cccgtctcca acgaggcctc gcgcaggagc tgttcaccgc gagggcgaac gccgacgtgg
     6841 ccggggctgc cgcacccgag cggcgctggg ctcgctggat cgcaccggcg gccaccgccg
     6901 cggccgtcct tgcgggggtc ctcgtgatga cgctgggggg cggcgccgac gatgccgccg
     6961 agaccgcgct ccagccgttc agccgcgcag gggagggggg ggcggacgcg gggggcgccg
     7021 acgactcggc ggcccagtcc caggcaggcg gccaggtcga ggacaagcgg ggacggcgac
     7081 gcgggctgac cgcggcgacc ggccaggctt cgaccgagtc ggacttcgcc gggcccgagc
     7141 agcccgcgcc ggtcttcgac cccgacgggg gcacgatcac gacgtccgag ctctcggaga
     7201 tcgggcgagg gcgcctgttc cggtcgttcg ccgattccta ccggccggag gacgggccgg
     7261 gtctgtacga ccgcttcctc gagcaactga ctacggacgc ggtcgcgctc ggcccccaga
     7321 tcgaggcgtg cgccgccacg ctcccgcagg acggctcgct catccccacg tacgcgaccg
     7381 tcggcgagta cgacggccgg cgcgcgctcg tggtcggctt cgtcaccggc gaccccggct
     7441 cgcggacgct cgaccgctac ctgatgtggg tgtgggagat cgacgtgtgc gagcagccga
     7501 tcgacacgtt gttcgagcgc atcgactcag gctgacgcgg ggaacatcgg accgctcggc
     7561 tcggttcgat agggtgtgaa cgagatgccc gagcagcgac gcgtgatcat catcgggtcc
     7621 ggcccggccg gcctgaccgc agcggtctac acgtcccgcg cgagcctcga accgctcgtc
     7681 atcgagggga tcgaggccgg cggccagctc atgctgacga ccgaggtcga gaactatccc
     7741 ggcttcgtcg acgggatcat gggccccgag ctcatggagg cgatgcgcaa gcaggccgcg
     7801 cgcttcggga ccgagttcct caccgacaac gtcacctccg tcgacttctc gcgcaagccg
     7861 ttcgtcgtcg agaccgcgtc gacgacctac gaggccggcg ccgtgatcat ctcgaccggt
     7921 gcgtccgcgc ggatgctggg cgtccccggc gagcgcgagc tcctcggcca cggagtgtcg
     7981 acgtgcgcga cctgcgacgg gttcttcttc cgcggccagg agatcgtcgt ggtcggcggt
     8041 ggcgactccg ccatggagga ggccaacttc ctgacgaagt tcgcgtcgaa ggtcacggtc
     8101 gtgcaccgcc gcgagtctct gcgtgcctcg aagatcatgc aggaccgcgc tcgtgcgaac
     8161 accaagatcg agttcgtctt cgactccgtc gtgaagcaga tcaacggcaa cggcaaggtc
     8221 gctagcgcga cgttgctgaa cgtgaagacg ggggccgaga cggaccttcc cgccgccggc
     8281 gtgttcgtcg cgatcggcca caccccgaac acgtcgctgt tcgagggcca gctcgacctc
     8341 tcgggcggat acatcgtcac gcacgacggg acggccacct cggtcgaggg cgtgttcgcc
     8401 gccggcgacg tcgtcgactt caggtaccgc caggcgatca cggccgcggg catggggtgc
     8461 atggccgcga tcgacgccga gcgcttcctc gaggacgtgc cgatcaccga gcgtttctgg
     8521 tagcgaaccc tcccggagct ctgcgagtct cgtagtcgtg cggtccctcc tctcgcgcgc
     8581 cctgcccgca gttctgctcg tggccgtgtc cgcccccgcg cctcgggcgc agccggcgcc
     8641 gcgcatggtc ttcccgatcg tgcggtcggg ctggtactcg gtgatcaact tcaccgacga
     8701 ctggcacgac ccccgcatgc gacggaccgc cgacgggtgg cagctgatcg gccgccacga
     8761 ggggaccgac gtcttcgccg agccgggcac gccggtcgtc gcgatcctcg ggggccgggt
     8821 cgagcagctc ggctggacct tctacagcgg ctggcgcgtg ggcgtgcgcg gagacgacgg
     8881 acgctactgg ttctacgcgc acctgagcag cttcgccccc ggcctctcga tcggcagccc
     8941 cgtcgcggcg gggcaacagc tcggcgaggt cggcaacacc ggctacggcg ccacccccgg
     9001 tcaccgggac gagttcacct accacctgca cctcgggatc caggagccgg acgggacctg
     9061 ggtcaacccc taccccctgg tccgccgcct ttacgatgct gcggtgcgaa gggacggagc
     9121 gtgacgagcg ggccggctcg gcgggacgcc gggatagagg gcctcatccg ccagggcgac
     9181 cggtcacagc aggtggccga cgtccaggcg cgcctgcgcg cgctcggcct ggccgtcgac
     9241 gacgaggccg gtgtcttcgg cgactcgacg cggcacgcgg tgaggacctt ccagcagacg
     9301 cgcggggtcc tggtcgacgg gatcgtcggg cctaacacgt ggaccgagct cgtcgaggcg
     9361 tcgtggcggc tcggcgaccg cagcctctac ctcaaggtcc cgccgttccg cggcgacgac
     9421 gtgtcgctgc tccagaagag gctgaacgcg ctcgggttcg actcgggccg cgaggacggg
     9481 atcttcggcg aggacacggc ccgcggcgtg aaggagttcc agaaggagta cggcgtcgcg
     9541 gaggacggca tctgggggcc gcgctcgcag agtgcgctcg cgggactgcg cgtcgatcgt
     9601 ccggggacga cggcgcagct gcgcgaagag ctccggcgct cgcccgacct gcacggcgcg
     9661 ctcgtcgtgc tggaccccgg ccacggtggc tccgacccgg gagcccgcgc cgcggcaggg
     9721 cttctggagg ccgacgtcgc gtgggacctc gcggtgaggc tcgcggacaa gctgagcgga
     9781 atgggggccc gggtgcggtt cacccggacc gaggcggagg cgccggacgc atccgagcgg
     9841 gcccgccgcg cgaacgaggt ggcgggcgac gtcttcctgt cgcttcatct caacgagcac
     9901 gaggagccga ccgccgaggg ggcctcgacc tactacttcc acacctcggc ggcgggacgg
     9961 tcgctggccg agtcgatcca ggaggggctc gtggcgctcg gcgtgcgcga ctgccgcagc
    10021 cacgcctgct cgtacacggt gctccgcgag acgcggatgc ctgcggtcct cgtcgagccg
    10081 gtgttcgtgt cgaaccccga cgaggcgaag aggctcgaag aggcggactt ccgggccgcg
    10141 ctcgccgacg cgatcgcgac cggcgtcagc cggtactacg cgtcgacgcc ggtctagtcg
    10201 ctgggaggcc ggccggttat cgccgcgacg atcctggcga ggtcgtcggg cgagacgaag
    10261 tccagcgtga tctttccctt gcgggcgccc atggtgacct tcacgcgcgt ctggaggtgg
    10321 tcgccgagcc tccgctgcgc ctcggcgacg gcccccgact cctcggccgc gcggcgtgac
    10381 ccggctctcc ccgccgtgac gcccccggtc atagcgaggt agcgcgcgac ctcgtccgcc
    10441 gtctcgcgca cggagagctt ctcctccgcg gcccggcggg ccatccgctc ctggaacggg
    10501 ttgtcggaca gccgcagcag tgcccggccg tgcccggcgc tgagcttccc ctcgatcagc
    10561 agacgctgca aggaggtcgg gaggtcgagg aggcggagcg cgttcgtgat cgtcaccctg
    10621 ttcttgccga ggcggtcgcc gagctcctct tgcgtcagcc cggcctcggc gatcagctgg
    10681 cggtaggccg cggcttcctc gatcgggttg aggtcggcgc ggtggatgtt ctcgacgatc
    10741 gcccgctcga gggagccgcg ctcgtcggtc tcgacggcga tcgcggggac ggtcttgagg
    10801 cctgccgcat gggccgcgcg cagcctgcgc tcgccggcga tgagctcgta ggtcccacca
    10861 ccaaggtcgc ggacgagcag cggctgcagg atccccagct cgccgatcga cgtcgcgagc
    10921 tcggcgatcc ccgcctcgtc gaacgtgcgc cgcggctggt gtgggttcgc gacgatcgcg
    10981 tcgaggggaa gctcgaggag gttgccgcgc gcctcgggcg cctcgcctcc gcctcccgac
    11041 ggaagcagcg cgtcgagccc gcggccgagt ccggacttcc tggtcacggg ccgggaaccg
    11101 tgaacggctc gccccggggc cagaccgagt cggggaggtc gggccggggt gtcttcaccc
    11161 cgtagccgcg gcccccgggc cccggagtcg acgggcggga ggcggtgagc accggtgcgg
    11221 gagccggcgg gggaggcgga ggcttgtgga cctcgaggcc gtacagggtc tcgacctcgg
    11281 cggcgagtag gcggtacgcg acggcgccgc gcgacgaggg atcgagcgtg agcacgggct
    11341 cgccgaacga gggggcctcg gacagccgga cgctgcgcgg gatgcgcgtc tcgaacacga
    11401 gctcgccgaa gtgggaacgc acgtcgttct cgacctggct cgacagcttc gtccggccgt
    11461 cgtacatcgt caggaggaag cccgcgatgc ggaggtcggg gttgaggctc cggcgcaccc
    11521 gctcggcgtt cccgaggagc tggccgaggc cttcgagcgc gtagtactcg cactggaccg
    11581 gcacgaagag gtcctgcgcg gccgccagtg cgttgacggt caagagcccc agtgacggcg
    11641 ggcagtccag gaagatcagc tcgtagcgct cggccgcgcc cgccagagcc tccccgagcc
    11701 gcctctcgcg cgccatcgtg ctgacgagct cgacctcggc ccccgcgagg tccagcgagg
    11761 ccggggcaca gtcgaggttc ttgatcgcgg tcggccggat cacgtcgtcg agggtcgcgt
    11821 cgcccaacag caggtgatac gtcgagcgct cgacgttccg gtggtcgatc ccgaggccgg
    11881 tcgctgcgtt cgactgcggg tcgacgtcga cgacgaggac ccggtggccc cgcagggcca
    11941 ggccggcggc caggttgacc gacgtcgtcg tcttggccac gccgcccttc tggttggcga
    12001 cgcagacgat ccggccgcgg cccccctgga tggggcgccg ttccaggagt ttccagttct
    12061 tggaggagtt gtcggtcact tcttccgcat gatagtcagc cccggctccc attcgtcggc
    12121 atcgaaaggg gcggcggcgt cggggccggt gaagacgagc gtgacgccgc ccgggctcaa
    12181 gagcggctcg gcgagctctc tcacggctgc cggatcggcc accgcgcggg ccgtcacgag
    12241 gacgtgggct cccgccaggc gggggtcccg ggccgcctgc tcggcggtcg cggcgacgat
    12301 ctcgcagtcg aggcccagcc tccggacggc ctcctcgagg aaagcggccc ggcgctgccg
    12361 gggctcgaga agggtccaat gccgctcggg tcgggcgatg gagagcggga tcccgggaag
    12421 cccggccccc gatccgacgt cgacgcacgg cccctcgggg agctcgtcga gcaggggggc
    12481 ggcccgcagc gaatcctcca cgtggcgctc ctcgaaccgc tcgaggtcac gaggcgacac
    12541 cagatcgagc cggggggccc actcgcgcac cagggcggcg taccggcgaa gtagctcgtt
    12601 cgggtccatg taaaacgtga aacgtacccc ggacgcgaaa gaggggccgt cttcggcccc
    12661 tctttcgaat ccttgtgttg cttttgtcta gcggaccacc cacgagggga tgacgacggt
    12721 cgaagccggc tggtccagct cgtacaggcc gtggccgaag ttcgcaccac ggatcgtggt
    12781 cgtcatcgtc agcgcctcga acgacttctt gtccagtgcg ccgtcgagct tgatctcgaa
    12841 ggggacctcg tagcgggacg aagcaggggt gacctggtac gaggccgtgg ccgtaccgac
    12901 gacgacctcc tcgccgccgg acacgccggt gaagtcgatg acgacctcga cgttgccgcc
    12961 ggcaagaccg ttgtcgggcg ccgcgcccag gaacgacgag accatctgga gcttgcccga
    13021 gatcggcttg gtcgcgtcca cggtgagcgg gagaccgtcg gcgaccggcc acacgtctgc
    13081 gactgcgtcg agctgaccgc tggcgattac cgcctcgttg aggaggccgc tctcgcccca
    13141 tccgcagagg ttgccctcgt cgacgccgtc cacgaggctg agccgtgcgt tgtcggcact
    13201 gtcggcgccc gcaccggtgc aggcgtcgcg acgcatgtag aaggtctggt cgacggccac
    13261 gggggccgca ggcttcggct tcttcttctt cttcgctgcc tcggcgacgc cgagagaacc
    13321 gactacgaga cccatggcca gcgcggtgac cgcaagcttg cgtgccctca ttgattgctc
    13381 ctcgatctag gccgcctccg ccgggcggta tccaggagag gttttacgcg gcaggccgag
    13441 ttcctgttcg cgcgcgctga cgggttcgtg gcgaggcccg acgatgtttc acgtgaaaca
    13501 tgcgggctac tcgccgcgga cgacgacctt ccggttcggc tccacgccct cgctgaagct
    13561 cgtcgccccg tcgatctcgg ctacggcgtc gtggacgacc ttgcgctcgt aggcggtcat
    13621 gggctccagc tcgatctcgg tcccgcgctc cttggcccgc tcggctatcg accgtgcgta
    13681 ctcagccagc gaggcccgcc gccgtgcccg gtacccctcg acgtcgacga gcacccggac
    13741 gcggccccgc agccgtcgct ggacggccgt ccgcgtgatc tcctggagcg cgtcgagcgt
    13801 ctggccccgg cggccgatca gcccgcccag ggcctcgccg gacacctcga cggtcgccgt
    13861 gtcgccctcg acgctcgagc tcacctcgcc ctcgagctcg agcgacttca gcaggccgga
    13921 gacgaactcc tcggccgtgg aggcgacgtc ttccggcgtc gccggctcga gctcctcctc
    13981 ggcccccgac tccgcaggcg ggtcgccctc caggggctgc tccagctcgg cgtccgcgta
    14041 cgactcttct cgttcctcgg tcacttcttc ctcttcttct tgctcgaagg atgtggcttc
    14101 gaggcggccc cctcaggccg cttgccgccg ttcggggaag ctttcgacgc gttcgccgtc
    14161 gtggccttcg tcgtggccgg ggtcttgccg gcccccttct tcgggagcgt cgcgccgcct
    14221 ccggcgccga gcaccaccgg cggatgggcg gcgaggatga tccgctgctg gacgatcgtc
    14281 cacaggttcg tcgtgaccca gtagatgacg acaccggccg ggaagttgaa cgagaacacc
    14341 atcagcagca gcggcatgac cttggtgatc atctgcatct gctgtgcctg cgggctgtcc
    14401 ggcgtcgagc gctgcatctg cttctgctgg tagtaggtcg tgaagcccat gaagagcacg
    14461 aggcccaggt agggcagggc ggtgacgaac ccgctcccgc acgcctcgaa cagggcggcc
    14521 gtgccctcgc cgacgtcgtg ctgacccgtc agggtcccgt tcgcggagca gttgaggtgg
    14581 atcccgagga agctgttgat ctggaccggg atgtcgcgca gcccgtcggc gagcgccgag
    14641 ttctcgacga cgtcgcccat gtacttcagc ggatcccgga tgacgtagaa gagcccgatc
    14701 agcacgggga actgcatgag gagcgggaag cacccgccga acgggttgac cccgtgctcc
    14761 ttgtagagcg ccatcagctc ttcgttcatc ttctggcggt cgcccttgtg cttcgcctgc
    14821 aggcgcttga tctcgggctg gatcgcctgc atctcgcgca tcgacttcgt ctgcttgatc
    14881 gagagcggga gcagcaggag ccggaccgcg agcgtcagca ggatgatcgc gaggccgtag
    14941 ctcgggatca gggcgaagaa cccggcgagg gcggtggaga agagctcgaa gaaacccatc
    15001 taggagggct ctccctcggg cgtcccgccg aggcggcccg cgcgcccgcg ggcaggcggg
    15061 acgtggtcga cgccacccgc ggaccacgga tggcagcgag cgacccgctt cacggccatc
    15121 cagccccccc ggaacgcccc gtgcgtctgg atcgcctcgg cggcgtaggc cgagcacgtg
    15181 ggagcgaagc ggcagtgagg gccgagcagc ggagagatcg cccgccggta gaacccgatc
    15241 agcgccacga ggacgcggcc cagggggctc acgacggcct ccggggcact ccggcccccg
    15301 aaaggctctg tcttacatat tcttccactc gggagaacgg ctcggtcgcg acctgccccc
    15361 ggcctacgag gacgacgtcg gcggaaccgg gcccgtaggc gcggaacgcc tcccggagac
    15421 ggcggcggat cttgttgcgg acgacggcct tgccggccga cttaccgacc gagagcccca
    15481 gccgggctgg cccctcgtcc ctgaccgaga cgaccgcggt cacgccgttt ttcgtgcagc
    15541 gcctgccggt ggcgtggacg cgcgcgaagg ccggcttgcc ggacaacggt tcgatggatg
    15601 gcatctcgcg agaaagggcc cgggatcagg ccgacagccg ctcgcggccc ttggcccgcc
    15661 gtgccttgag gatcgccctg ccggcccggg tcttcatccg ggcgcggaag ccgtgcttgc
    15721 gcttgcggcg gcgcgtgttc ggctggaacg ttctcttcgt catgtggaca tcttccgttc
    15781 ggttcgatcg cggcgcgatc ctcgagagga tcgcttgcgg ccgaggcggc tatcggggtc
    15841 gcggcagcgc tggccgtccg aggtgccgag cgagtatagc gggcgcgccc cgtaccgact
    15901 tcgggcactt ttcgaggttt cctcgcgacc cggggggtct cgccttgacg ccctccggca
    15961 gcgcctgctt aaactgccga gcggtcttca agccctaaga ggacgggcag cagggcacgt
    16021 ggcacttccc cacggattca ccggctgagc accagccgag gcggcgtcgg cagactcgtc
    16081 cacacctgtg gataggcctg ttagtcactg catcggccct gctcagaccc actattccct
    16141 ctccctgccg gcagcgcgcg ggttgctgcg gccctcgggg cggaacctcg cgggccgcgc
    16201 cgggatgtgg aaaacgagca tcgatgacgg ggagggcatg gatgcaaagc tctattagcg
    16261 aagattcacc cgcgcctgcc acaagcagta cgggcgacac cgccgctgcg atctgggagc
    16321 gcgcgacaga ggacctcaag ctcgccctgt cggagagcaa cttctccgca tggttcgggc
    16381 ggacgcaggc ggtcgggctc gacggggaca cgctcgtcct cggcgtcccg aaccacttca
    16441 cgaaggagtg gatcgaaggc cgcttcctcg agcccctggt cgccgcggtc acgaccgccg
    16501 ccggccgctc gctcgaggtc gccatcctct ccgacgccgc ctacgccacc ggcgacccgg
    16561 ccccccaggg accggccccc gaggtgatca cgctcgcgga gtcccccgag gtccccgcgg
    16621 acgacgtctt ccacccgaac tacaccttcg actcgttcgt catcgggtca tcgaaccgct
    16681 tcgctcacgc ggccgcgctg gccgtcgccg aagctcccgc ccaggcctac aacccgctgt
    16741 tcatctacgg cggcgctggc ctcggcaaga cgcacctcct ccacgcgatc ggcaggtacg
    16801 cgcaccagtg gcagccgaac accgtcgtgc gctacgtgtc gaccgagcag ttcatgaacg
    16861 acttcatcct cgccctccag cgtcgcaaca tcccggcgtt ccaccgccgc taccggatgg
    16921 ccgacctcct gctcatcgac gacatccagt tcctcgaggg caaggagcgg acacaggaag
    16981 agttcttcca cacgttcaac gcgctgcatc ccaagagcca gatcgtcatc acgtccgacc
    17041 gtcctccgaa gaagatctcg acgctcgaag aacgtctccg cacgcgtttc gagtgggggc
    17101 tcatcaccga catccagccg cccgatctgg agacgcgtct cgcgatcctc cagaagaaag
    17161 ccgagaccga gcagctcgtc gtcggacacg aagtcatgtc gttcatcgcg agccgcatac
    17221 agacgaacat ccgcgagctc gaaggcgccc tcatccgcgt cgccgcctac gcgtcgctca
    17281 cacgctccga tctcaccttg gacctagcgc aacaggtgct ccgaaccctt ctcccggagt
    17341 cacgagaggc gcgcgtcacg tccgacctcg tcgtctcggt cgccgcggag tactacgacg
    17401 tcacccccga ggagatctgc tcttcgagcc ggtctcgccc tctcgtcaac gcacgacaga
    17461 tggcgatgta cctctgccgc gagctcacgg acctctccct tccgaagatc ggcgagcgct
    17521 tcgggggccg cgaccactcg acggtcctgt acgccatgca caaggtccgt cagcacatga
    17581 aggaacgtca gagctgctac cagcaggtag aggagctcac gacgcgcatc cgccaacagg
    17641 ccgcccgtgg ataagacgtc acgcgcctgc gcggaatgtg gaaccaacgg cgtcttcttc
    17701 gcgaacgacg tcattcgtcc ttcggctacg agtgtggtcc cacaacgcgc gcgcatcgcg
    17761 aaacccgctg ctaccagggc cgatgcgcgt tatccccgta gttcacaggc cctactaagg
    17821 actactgaaa gatctaaatc agtaccccaa cccctgaacc tcaaaacctt gtcacacccc
    17881 gtctctaccc ttggatctac aagcaacccg atgggaggac cacgatgaag ttccgctgcg
    17941 agcgcgacga cttgctcgag gccgtgcagt tcgcgtcgag ggctatctcg aaccgcgcga
    18001 cgttgccggt gctgtccggg ttgcggatcg acgcgaccga ggaaggcgcg gtcaccgtcg
    18061 cggcgacgga cctcgagctc acgatggaga cgtcgttcca ggccggcgtc gacgagcccg
    18121 gcaagacgat cgtcccgggc cggctgttcg gcgagatgag ccgcagcctg tcgtccggcg
    18181 cggtcacggt cggcggtggt gaaggcgagg tcgagatcgg ttcgggccgc gggaacttcc
    18241 gcgtgaagtc gctcgcgccc gacgactacc cggcgctccc gatcgagggc cgcgaggacg
    18301 cgggtgacgg tgtcgtggtc gacgtcgacg ggaacctgct cgcgacagcg ctgtcgcagg
    18361 tagtgcgcag cgcgtcgggc gacgagagcc ggcaggtcct gaccggtgtc ctgtgggagc
    18421 tcgagtcggg gagcctgacc ctcgcggcga cggactcgta ccggctcggc gtgcgtaccc
    18481 tgaaggtcga cgggggcccg gccgagctgc gcaaggtgat cctgccggcg cgggcgttgc
    18541 acgagctcgc acgtgcgctg cagggcgggg gctcgagcgt gaaggcgacg gtgaaggaca
    18601 acctcgtggc gttcgcgatc ccggccgccg gtgaagggtc gggcgggggc gagtccgtca
    18661 tcggcagccg cttcatcgag ggcgagttcc cgaactaccg tcaactgatc ccgcagggtt
    18721 acccgaatgc tctgacggtc gagcgcgagg cgctgctcga ggtgacgaag cgcgtggggc
    18781 tgctggcgca gaacaacctg ccggtcaagc tccagctggg ggccgagctc gaggtgtcgg
    18841 cacacacacc cgacgtcggc gagggccagg aggtcatcga cgccgagtac cagggcgaac
    18901 ctctggtgat cgcgttcaac ccgcagttcc tccacgacgg cgcctcggcg atccagtccg
    18961 agaagatcgt catcgaggtg tccgacgggt tgaagccggc gatcctgcgc ggcgagagcg
    19021 acgaggcgtt cacgtacctg ctcatgccgg tccgcctgtc gtaggcgatt cggccatagg
    19081 gtcgagaggt gcgtgtagat gctctcgccc tcgaggactg gcggaactac gaacggcagt
    19141 cggtcgacct gtcgcccggg ctgaacctcg tggtcggcgc gaacgcgcag ggcaagacga
    19201 acctgctcga ggcggcttat tgcctgagcg gcttcggctc ccctcgtgcc gcggacgcgg
    19261 ccctcgtgcg gcacggttgc gagcgcgcgc tgctgcacgc gtccgtcgaa cgccgggggc
    19321 ggaccgtccg gatcgacctc gaggtacgac cgggccgcgg gtcgcgcgcg ttgttgaaca
    19381 agactccggt gcagccgcgg ctcctgggcg acgtgtgcgt cagcgtgttc ttcgggccgg
    19441 acgagctgtc gctcgtgaag ggctcgccgg acggacgccg gcgcgtcctc gacgagctga
    19501 tcgtcaagct gcgccccgcg cgcgacggga tccggcgcga gttcgagcgg acgttgaagc
    19561 agcgcaacgc gctgctgaag tccgcgccgc gcgggggctc cgcgacggtg tcgtcgacgc
    19621 tcgacgtatg ggacgcggcg ctggcgcgtg cgggggccgc gctcgcggcg gcgcgtctcg
    19681 aggcgttgac cgggctgatc ccgctcgcgg ccaagaggta cgccgaggtc gcgggggccg
    19741 ggacgttgac gctcgagtac gcgagctcgt gggtgggcga gaacacggct ccggacgcgg
    19801 cgtcgctgga agagcagcta cgcgccgcga tcgccgaggc gcgaccgcgt gagatcgagc
    19861 gcggcgtcgg gctcgtcggc ccccagcgcg acgacgtgtc tgtgatcctc gacggcgtcg
    19921 acgcgcgggt gttcgcgtcg cagggggacc agcggacgtg cgcgctcgcg ctcaagctcg
    19981 gcgagcacga cctcctgtcg gacgcgctgg gcgaggagcc cgtgctgctc ctggacgacg
    20041 tcttctccga gctcgacccg tcgcggcggg cgtggctcgc cgacgcggtc aggtcgatcg
    20101 gccagacgct ggtctcgacg gccgagccgg tcgcgctcga cgccctgtcg ccgggagcga
    20161 cgttctcggt cgccggcggg acggtggtcc gcagtgcgtg acgagccgtt gcggctgaag
    20221 gacctgctgc cggacgcgac cggccggctc ggcatcccct cgcccgaggc gacgggacgc
    20281 gtgtggtccg cctggaagga catcgtcggg cccgccgtcg ccgctcatgc cgagccgacg
    20341 tcgctgcgcg gcggggtgct gagggtccgc gcggactcgc cggcgtgggc gaccgagatc
    20401 gggtacctcg tcgacgagat ccggagccgg gcgaacgcgg tcacgggccg gccgctcgtc
    20461 tccgaggtga gggtctggac gggacccggg agcacgctgc cggcgggggc cggggctacg
    20521 gtcgagccgg cctccgagga gcccgagcgg gagcgtccgc aggaccccgc cgaggcgctc
    20581 gaacgggcgc gccgggcatg ggcccgccgc cgccggcggt gaccctcccg agacccccca
    20641 aaatcccgta aatcccctgg tagatggtag gatttcgccc gtgaacccac gcttcgaacc
    20701 ctcgcctcgc cgcgccctcc ggcacctgtg acgagcgcct tccgacgtgt agcggcggcc
    20761 actgccgccc ccgggttcga ccgctttgga tagaggagca tgaccgtagc cagcgacgca
    20821 cgcgaacccg caaagaaacg ccgcgacggc tcctataccg gccaggacat cacggtcctg
    20881 cgcggcctcg accccgtccg caaacggccc ggtatgtaca tcggctcgac gggcatccgc
    20941 gggctccacc acctcgtgta cgaggtcgtg gacaacgccg tcgacgaggc catggccggg
    21001 tactgcgacc ggatcgtcgt cgagctcctc gccgacgggg gatgccgggt ctcggacaac
    21061 ggccgcggga tccccgtaga cccgatcgag acggacggcc gcaagaagct ccccgccgcg
    21121 acggtcgtgc tcacgaccct gcacgcgggt gggaagttcg agggcaaggg ctaccaggtc
    21181 tccggaggcc tccacggcgt cggcgtctcg gtcgtcaacg cgctgtcgga gcgtctcgag
    21241 ctcgagatcg tccgcgaggg ctcgctgcac cgccaggagt tcgaacgcgg gacgccgctc
    21301 ggccccctga cgaaggtgaa gccggcgaag cggaccggga cgacggtcgg gttctggccc
    21361 gacccgttga tcttcacgga agagcgcgag ttcaagcagg agaccctcgc ccagcggttg
    21421 cgcgagatgg cgttcctcaa ccgcggcctc gagatccgcc tggtcgaccg ccgcgtcgag
    21481 ccggcgaccg aggagacgtt ccgctacacg ggcgggatcg tggacttcgt gaagcacctg
    21541 aactcctcgc gcgacgcggt gaacaagcgc ccggtgttct tcgacgcgaa ggccgagggg
    21601 cacgaggtcg aggtcgcgat gcagtggacg acctcgttca acgagtccgt cttcacgttc
    21661 gcgaacaaca tcaacacgca cgaaggcggc atgcacgaag agggcttccg ccgcgcgctc
    21721 acgcgtgtga tctcgaagta cgcgcgcgac aagaacatcc tcaaggagaa ggacgacgcg
    21781 ttcaccggtg aggactgccg cgagggcctg accgcgatcg tctcggtcaa gctccgcgac
    21841 ccccagttcg agggccagac caagaccaag ctcggcaaca ccgagatccg ctccttcgtc
    21901 gagacctcga tgaaccagcg cttcgcggaa ttcatggagg agcacccttc cgaagcccgc
    21961 gccatctgca acaaggcttt gtccgcccag cgcgcgcgcc tcgaggcgcg gaaggcgcgg
    22021 gacctcgtcc ggcgcaagtc gttgctcgag tcctcgacgc tccccgg
//
LOCUS       DASQDO010000100         9508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_194434, whole genome shotgun sequence.
ACCESSION   DASQDO010000100 DASQDO010000000
VERSION     DASQDO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194434"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VEV43_03145"
     CDS             complement(<1..325)
                     /locus_tag="VEV43_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22546.1"
                     /translation="MSLLLTLLLLLPAPAAYREAAPPAPRIDYSSYLGGSGDESLAIA
                     AAPDGSVYVAALTTSRDLPVRGGSGPGARFDVWVARLSPDGRRLRWSTYLGGLGVDTL
                     GGLAVD"
     gene            388..1587
                     /locus_tag="VEV43_03150"
     CDS             388..1587
                     /locus_tag="VEV43_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22547.1"
                     /translation="MQAPRRHRSLRDLILVQGSGAGGDALVALALAGTLFFSVPEATA
                     RGRVALYLALTVAPFAVIAPLLSRVLDLHRGGLRWALVIAAAGRAALAWWMATRLDSL
                     YLFPLAFGILLLSRAATVVRGAVLPHLVPEEDELVAANASLTRFAAVAGIVAVGPGLL
                     FARFIGNGTVLVTGAIFYGLGVVPAFRLPSPKGRRESGETMSARRLARSVAVRQATVA
                     SAGMRFLVGFLVFHLAFALRREDAGSIGLGLLIAAAAIGGLAGSLVAPPLRRRLAEEA
                     IIVASLVVAGVAGLMVGNWFSLFAAAGLVFFIGMTSGTAKLAFDSIVQRVTPEGGRGW
                     AFARFESILQLAWVGGALIPLIVPIPGGPGVAGAGVTAALLASVYALGRSRVKRRSGW
                     IASARGK"
     gene            1629..2813
                     /locus_tag="VEV43_03155"
     CDS             1629..2813
                     /locus_tag="VEV43_03155"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HYP22548.1"
                     /translation="MNIIFKRNDAPTLGAEIEVQIVDAAGALATDTAATKILAQLGDD
                     PGYKHELLECCIEVITGVCPTVGAVRKDLGDKVERLIEVADGQGYRIMCTGTHPFSSW
                     ADQTVSPDPRYHRLIEDCQWTARRLLIFGVHTHVGVGSGEEAIAISNALATYIPHFLA
                     LSSSSPFWQGRDTGLASIRSKIFESLPTAGLPYFMENWGQFQRFMRTLIGAGTIRSIR
                     EVWWDIRPHPGFGTLELRICDGIPTMDELCSMVALSQCLVVWMGDRYNAGLPLPQHKA
                     WTVRENKWRAARYGLDAEIIRDEEGNLLSLRRSIGDTIEMLEPIAERLGCHEDLCRVN
                     DILERGTSAVRQREVYGRSRDMSQVVDALVEEMTTGKPQSLPDDSLVGGTVVHGDPLP
                     GE"
     gene            2850..3221
                     /locus_tag="VEV43_03160"
     CDS             2850..3221
                     /locus_tag="VEV43_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP22549.1"
                     /translation="MKKTLFAAAVLLALPSCGGSEPDAAAVTVEHVAFAPAALEVDAG
                     TEVVWTNRDAEVVHTATSGVPGDKGVPGLDNGTPARPDGTFDGEMDGADASFSFTFDE
                     PGTYAYFCRVHQSMTGEIVVR"
     gene            3307..4602
                     /locus_tag="VEV43_03165"
     CDS             3307..4602
                     /locus_tag="VEV43_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22550.1"
                     /translation="MEVRVRKTWLRLFAPLVALSLFGAACASGEDAATDTGAQAPAAE
                     SDDAMASMEASSSSDAPAVDTPAATLTRDLTSLLAGHEYHAGLAIYTAVQAGGDLKDP
                     TVQAAVKSLDKNTQALAGAVGSIYGKPAEQQFLKLWRAHIGFFVDYTLGKATGNAQMA
                     QTAQRNLDGYRNDFGALIEGATEGALTQEQVAEALIPHVESTSAAIDAVVAGDASAFD
                     KLQEAAHHLPMIATALSTGISTQQDLEGNPDDAAAQLQRDLTSLLAGHEYHAGIAVYT
                     AVQAKGDLKDPTVQAAVKSLDKNTQALAGAIGSIYGEAAEQQFLKLWRAHIGFFVDYT
                     LGKATGNAQMAQTAQRNLDGYRNDFGALIEGATEGGLTQEQVAEALVPHVESTSAAID
                     AVVAGDASAFDKLQEAAHHLPMIATALAGAIVEQFPDKF"
     gene            4766..5167
                     /locus_tag="VEV43_03170"
     CDS             4766..5167
                     /locus_tag="VEV43_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22551.1"
                     /translation="MRKLVAAIAAAAVVTLLVAPATAAGTKSGCKGKWTDAGVCSFTY
                     KGGSLHVQASGAGGDPATIVSIRLEAAGPAPGTNVLVLSCGVAAGPQGAPAACGGAST
                     GGEAYQPEPGDELTCTVTGNGTGKYRCASGI"
     gene            complement(5192..7024)
                     /gene="selB"
                     /locus_tag="VEV43_03175"
     CDS             complement(5192..7024)
                     /gene="selB"
                     /locus_tag="VEV43_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HYP22552.1"
                     /translation="MQVIGTAGHVDHGKSTLVRRLTGIDPDRFEEEKRRGLTIDLGFA
                     WVQLPSGLEAGVVDVPGHEKFVRNMLAGAGGITVCLFVVAANEGWMPQSAEHLAILDV
                     LGVSRGVVALTKADAVDEETVAIATEEVEERLSATSLAGSTVVPCSATTGEGIPELLA
                     ALDDAVSSAPVPPDTGSPRLWVDRVFTIAGAGTVVTGTLMRGSLRAGDEIEIAPEGRR
                     ARVRAIQSHKKELSEIGPGNRVALNLAGLARQGAERGDAVVRPGKWRATRRADVKLRV
                     LAQDVTGHDVALTEKGAHLLYAGSAETPVRLKLLGTSKVRPGEDAFAQLRLPAPLPLV
                     PGDRFVIRDAGRVLTLGGGEVLDPLAPDARRSDEARLSLMQELASGDAGSRLAALVRA
                     YGGLDADDALFRSGARAPGDGVVRLGSWIVSEERKAELIASVLAAVEEHHRARSLERG
                     MPREALKQSIPLPPEAFDALLDGTGEIVQEGALVRAVAHRVSFAPEQDAARKKVLEEI
                     EAAGFTPPLAKDLSGDRSLLRALVDGGDLVAVGDFYLTRGQAETAKRRVVEAISGGTG
                     LTVAEIRDLLGTTRKYAVPLCEWLDATGVTRRRGDVRVLGPRAG"
     gene            7082..7279
                     /locus_tag="VEV43_03180"
     CDS             7082..7279
                     /locus_tag="VEV43_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22553.1"
                     /translation="MQTLERPRAHMPARPQPREPISDPVPSPSPEPGPEPRQPGPAPS
                     TEPVPPQDPDREEADTTGAER"
     gene            7276..7923
                     /locus_tag="VEV43_03185"
     CDS             7276..7923
                     /locus_tag="VEV43_03185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYP22554.1"
                     /translation="MIAAILDIDGTLVDTNYQHALAWYRAFKAYDVIVPVWRIHRHVG
                     MGGDHLITALTDEEIEERLGDDIRAAEKKLYAELIDEVAPMEGAAELVSDLKERGHRV
                     VLASSAKEEEVDHYLDLLGVRDAVDGWTTSADVEATKPEPDLVEAAVEKSGAGKAVMV
                     GDSIWDCEAAERAGLPTIGVLTGGFARSELEERGAAAVFESVEELRRNLNDTPLR"
     gene            7937..8629
                     /locus_tag="VEV43_03190"
     CDS             7937..8629
                     /locus_tag="VEV43_03190"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HYP22555.1"
                     /translation="MSHHEKKIVKLLSEAHANELALVQTLEAHKRVAPEGPYLDRIDQ
                     HVDETRNHAERMQRRLDQLGATRNPVEAGVALVHSFIQNTLAMAKAPVDMVRGSFNVE
                     EKALKNARDEIVTEALEIAQYEAIEHMAIALGDDVTARLAADIRADEEAMLADLRAQI
                     PTLVAGVVRASVPADEIVLDAVIPWPGYDDMTVDEIKERLASSPELGDAVEAYERAHK
                     NRVSIVRELQDA"
     regulatory      8745..8830
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8845..9432
                     /locus_tag="VEV43_03195"
     CDS             8845..9432
                     /locus_tag="VEV43_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22556.1"
                     /translation="MHRSATRTLTVVALTGLLAAASLPAQAKSRHKEHRGKKAMGEIV
                     SFDEATMTLEVDLPGDEDFSGAVDPDARVKLDHRGWNKRGGSHGNPTEGSLDDLLPGA
                     AVLRMKVEDDLVTKIRIRPIAEAPLPPAEDGDDEDALPGDETPADDTPGDDSTDDSED
                     DSDDDSDDEDADTNPDDEDLGDPEDALPPLPVPQP"
BASE COUNT         1470 a         3396 c         3325 g         1317 t
ORIGIN      
        1 gatcgaccgc caggcccccg agggtgtcga ctccgaggcc cccgaggtac gtcgaccatc
       61 gcagccgccg tccgtccggc gagagccgcg cgacccagac gtcgaaccgc gcccccggcc
      121 ccgacccgcc ccgcacgggg aggtctcgcg acgtcgtcag ggcggcgacg tagacggaac
      181 cgtccggcgc cgctgcgatc gcgagcgact cgtcgcccga gccgccgaga tacgaggagt
      241 agtcgatcct cggagcgggt ggggcggcct cccggtacgc ggccggggcg ggcaacagca
      301 gcaacagcgt caggagtagc gacatgtccg tgggactccc ggcggggccg cagcgttccc
      361 gtcacaggcc tcctgtacgt tctcgctgtg caggctccgc gaaggcatcg ctcgctgcgg
      421 gacctcatcc tggtccaggg atcgggagcc ggcggcgacg cgctcgtcgc gctcgctctg
      481 gcgggaacgc tgttcttctc ggtccccgag gccacggcgc ggggacgcgt ggctctgtac
      541 ctcgctctga ccgtcgcgcc gttcgcggtg atcgcgccgc tgctgtcgcg ggtgctcgac
      601 ctccaccgcg ggggactgcg gtgggcgctc gtgatcgcgg ccgccggccg ggcggcgctc
      661 gcgtggtgga tggcgacgcg gctggactcg ctctacctgt tcccgctcgc gttcgggatc
      721 ctgctgctgt cgcgggctgc gacggtcgtt cgcggggcgg tcctgccgca cctcgtcccc
      781 gaggaggacg agctcgtcgc cgcgaacgcg tcgctcacgc gcttcgcggc ggtggcgggg
      841 atcgtcgcgg tcggccccgg cctcctcttc gcgcgcttca tcgggaacgg gacggtgctc
      901 gtgacggggg cgatcttcta cggactcggc gtcgtgccgg cgttccggtt gccgtcgccc
      961 aaggggaggc gcgagtcggg cgagacgatg agcgcgcgtc gcctcgcgcg ctcggtcgcc
     1021 gtgcgccagg cgacggtcgc gtccgcgggg atgcggttcc tcgtcggctt cctcgtgttc
     1081 cacctcgcgt tcgcgctgcg gcgggaggac gcaggctcga taggcctcgg cttgctcatc
     1141 gcggccgcgg cgataggcgg gctggctggc tcgctcgtgg ccccgcccct gcgacggcgg
     1201 ctcgcggagg aggcgatcat cgtcgcgtcg ctcgtcgtcg ccggggtcgc agggctgatg
     1261 gtcggcaact ggttctcgct gttcgccgcg gccggcctcg tcttcttcat cgggatgacg
     1321 tcggggacgg cgaagctcgc gttcgactcg atcgtccagc gcgtcacgcc cgaggggggc
     1381 cgcggctggg cgttcgcacg cttcgagtcg atcctccagc tcgcctgggt cggaggggcg
     1441 ctgatcccgc tgatcgtgcc catccccggg gggccgggcg tcgcgggggc cggcgtgacg
     1501 gccgcgctgc tggcatccgt ctacgcgctg gggcggtccc gggtgaagcg acggtctggt
     1561 tggattgcct cggcgcgggg aaagtgacct gggcatcgcg ggagatcaat atctaggggg
     1621 gcagccctgt gaacatcatc ttcaagcgga acgacgcgcc gaccctcggc gccgagatcg
     1681 aggtccagat cgtcgacgcg gcgggcgcgc tcgccacgga cacggcggcc accaagatcc
     1741 tcgcccagct gggcgacgac cccggctaca agcacgagct cctcgagtgc tgcatcgagg
     1801 tgatcaccgg cgtctgcccc accgtcggcg ctgtgcgcaa ggacctcggc gacaaggtcg
     1861 agcgcctgat cgaagtcgcg gacggccagg gctaccggat catgtgcacg ggcacgcacc
     1921 cgttctcgtc gtgggccgac cagaccgtgt ccccggaccc ccggtaccac cgcctgatcg
     1981 aggactgcca gtggaccgcg cgccggctgc tgatcttcgg cgtgcacaca cacgtcggcg
     2041 tcggctcggg cgaggaagcg atcgcgatct cgaacgcgct cgcgacctac atcccgcatt
     2101 tcctcgcgct gtcgtcgtcg agcccgttct ggcagggccg cgacaccggc ctcgcgtcga
     2161 tccggtcgaa gatcttcgag tcgctgccga ccgccggcct cccgtacttc atggagaact
     2221 gggggcagtt ccagcgcttc atgcgcactc tgatcggcgc cggcacgatc cgcagcatcc
     2281 gcgaggtgtg gtgggacatc cgcccgcacc ccggcttcgg caccctcgag ctccgcatct
     2341 gcgacggcat cccgacgatg gacgagctgt gctcgatggt cgcgctgtcg cagtgcctcg
     2401 tcgtgtggat gggcgaccgt tacaacgccg ggctcccgct tccgcagcac aaggcgtgga
     2461 ccgtccgcga gaacaagtgg cgcgcggcgc gctacgggct cgacgccgag atcatccgcg
     2521 acgaggaggg caacctcctg tccctgcgcc gttcgatcgg cgacacgatc gagatgctcg
     2581 agccgatcgc cgagaggctc ggctgtcacg aggacctctg ccgcgtcaac gacatcctcg
     2641 agcgcggcac ctccgcggtt cgtcagcgtg aggtctacgg gaggagccgc gacatgtcgc
     2701 aggtcgtcga cgcgctcgtc gaggagatga cgaccgggaa gccgcagagc ctcccggacg
     2761 actccctcgt cggcggcacc gtcgtacacg gagacccgct gccgggcgag tgaccaatcc
     2821 cgcgacgccg ttccttacga acggcgtgca tgaagaagac actgttcgct gctgcggtct
     2881 tgctcgcgtt gccgtcttgt ggaggttcgg agcctgacgc ggccgcggtg acggtcgagc
     2941 acgtcgcgtt cgcgccggcg gcgctcgagg tcgacgcggg gacagaggtg gtctggacca
     3001 atcgcgacgc ggaggtagtc cacaccgcga cgtcgggcgt ccccggtgac aagggcgttc
     3061 ccggcctcga caacggcacg cctgcgcgac cggacgggac gttcgacggt gagatggacg
     3121 gcgcggacgc gtcgttctcg ttcacgttcg acgagcccgg cacctacgcg tacttctgcc
     3181 gcgtgcatca gtcgatgacg ggcgagatcg tcgttcgctg aaccaatccg cgcggactag
     3241 cccgtgcgaa ccacttccgc aagctcacga gggcccgttc gggcccgaga caacacgaag
     3301 gagttggtgg aagtgcgcgt ccgcaagaca tggctacgtc tcttcgcccc actagttgcc
     3361 ctatcgctgt tcggcgccgc gtgtgccagc ggtgaggacg ccgcgacgga taccggggcc
     3421 caggcccccg cagcggagtc cgacgacgcg atggcctcga tggaggcgtc gtcctcgtcg
     3481 gacgctcctg ccgtcgacac ccctgccgcg acgctgacgc gcgacctgac ctcgctgctc
     3541 gccggtcacg agtaccacgc ggggctcgcg atctacacgg cagtccaggc cggtggcgac
     3601 ctgaaggacc cgacggtcca ggccgccgtc aagtcgctcg acaagaacac gcaggcgctc
     3661 gccggagcgg tcggttcgat ctacggcaag ccggccgagc agcagttcct gaagctgtgg
     3721 cgtgcgcaca tcggcttctt cgtcgactac acgctcggca aggcgaccgg gaacgcgcag
     3781 atggcgcaga ccgcacagcg caacctcgac gggtaccgca acgacttcgg tgcgctcatc
     3841 gagggtgcaa ctgagggcgc gctcactcag gagcaggtcg cggaggcgct gatcccgcac
     3901 gtcgaatcca cgtccgcagc gatcgacgcg gtggtcgcgg gtgacgcgtc ggcgttcgac
     3961 aagctccagg aggccgcaca tcacctcccg atgatcgcga ccgcgctctc gacgggcatc
     4021 tcgacgcagc aggatctcga gggcaacccc gacgacgcgg ccgcgcagct ccagcgtgac
     4081 ctcacgtcgc tgctcgccgg acacgagtac cacgcgggca tcgccgtcta caccgcagtg
     4141 caggcgaagg gcgacctgaa ggacccgacg gtgcaggccg cggtgaagtc gctcgacaag
     4201 aacactcagg cgctcgccgg cgcaatcggc tcgatctacg gtgaggccgc cgagcagcag
     4261 ttcctgaagc tgtggcgtgc gcacatcgga ttcttcgtcg actacacgct cggcaaggcg
     4321 accgggaacg cgcagatggc gcagaccgcg cagcgcaacc tcgacggcta ccgcaacgac
     4381 ttcggtgcgc tcatcgaggg cgcgacggag ggtgggctga cgcaggagca ggtcgccgag
     4441 gccctcgtcc cgcacgtcga gtccacgtcc gcagccatcg acgcggtcgt cgcgggtgac
     4501 gcgtcggcgt tcgacaagct gcaggaggcc gcgcaccacc tcccgatgat cgccaccgcg
     4561 ctggccggcg cgatcgtcga gcagttcccc gacaagttct aacggtctcc ccacaggaag
     4621 ggcgctcgga gacgggcgcc ctttctgtat gtcccggccg tgacgaaatg cgtcgcccgg
     4681 cgtattccct cgatgaagcg tgcagggaag caagcacctg cgccgtagat cgaacggcgg
     4741 acgacggacc tggaggtgac cgaagatgcg caagctagtg gcagcgatcg cggcagcggc
     4801 cgtggtgacg ctcctcgtcg cgccggcgac ggcggccggc acgaagagcg gctgcaaggg
     4861 caagtggacc gacgccggag tgtgttcctt cacgtacaag ggcggcagcc tccacgtgca
     4921 ggcgagcggt gcgggcggcg accctgcgac catcgtcagc atccggctcg aggccgcggg
     4981 cccggccccc ggcacgaacg tcctcgtgct gtcgtgcggc gtggcggcgg gcccgcaggg
     5041 ggcccctgct gcgtgtggcg gcgcgtcgac cggaggcgag gcgtatcagc cggaacccgg
     5101 cgacgagctc acttgcacgg tgacgggcaa cggcacgggc aagtaccgct gcgccagcgg
     5161 gatctagaga acgctcacgg ctcggccccc gtcagcccgc gcgggggccg agcaccctta
     5221 cgtcgccgcg gcggcgcgtc acgccggtcg cgtcgagcca ctcgcacagg gggaccgcgt
     5281 acttcctcgt cgtgccgagc aggtccctga tctccgcgac cgtgaggccc gttccccccg
     5341 agatggcttc gacgacccgc cgcttcgccg tctcggcctg ccctctcgtc aggtagaaat
     5401 cgccgacggc gacgaggtcg ccaccgtcga ccagcgcgcg cagcaacgat cggtcgcccg
     5461 ataggtcctt ggccaacggg ggcgtgaagc cggcggcttc gatctcctcc agcaccttct
     5521 tgcgcgcggc gtcctgctcc ggtgcgaacg acacccgatg cgcgactgcg cgcaccagcg
     5581 caccttcctg gacgatctcg ccggtcccgt ccagcagcgc gtcgaacgct tccggcggaa
     5641 gcggtatcga ttgcttcaac gcttcgcgcg gcatcccccg ctcgagcgag cgggcgcggt
     5701 gatgttcctc gaccgcggcc aggacggagg ctatgagctc ggccttccgt tcttcggaca
     5761 cgatccacga ccccagccgt acgacaccgt cgcccggcgc gcgggcaccg ctgcggaaca
     5821 atgcgtcgtc cgcgtcgagg cccccgtacg cgcgcacgag cgcggcaagg cgggacccgg
     5881 cgtctcccga cgcgagctct tgcatcaagc tcagccgtgc ctcgtccgag cgtcgcgcgt
     5941 ccggcgcgag ggggtcgagc acctcgccgc cgccgagcgt gagcacgcgg cccgcgtcgc
     6001 ggatcacgaa gcggtcgccc ggaacgagcg gcaggggggc cgggagccgg agctgcgcga
     6061 acgcgtcttc gcccgggcgc accttcgagg tgccgagcag cttgagacgg acgggagtct
     6121 cggccgagcc cgcatagagg aggtgcgctc ccttctcggt gagcgcgacg tcgtggccgg
     6181 tcacgtcctg cgcgagcacg cgcagcttca cgtcggcacg cctcgtcgct cgccacttcc
     6241 cgggacggac gactgcgtca ccccgctcgg ccccctgccg cgccagcccc gcgaggttga
     6301 gcgcgacgcg gttgcccggc ccgatctccg agagctcctt cttgtggctc tggatcgcgc
     6361 ggacgcgagc gcggcgacct tccggcgcga tctcgatctc gtccccggcg cgaagggagc
     6421 cgcgcatcag cgtcccggtc acgacggtcc ccgcgcctgc gatcgtgaac acgcggtcca
     6481 cccacagccg cgggctcccg gtgtcgggag gaacgggcgc cgacgagacc gcgtcgtcga
     6541 gcgcggcgag caactcgggt atgccctcgc cggtcgtcgc cgagcagggc acgaccgtgc
     6601 tgcccgcgag actcgtcgcg ctcagtcgct cctcgacctc ctcggtcgcg atcgcgaccg
     6661 tctcctcgtc gaccgcgtcg gccttggtca aggcgacgac gccgcgcgac acgccgagca
     6721 cgtcgaggat cgcgaggtgc tcggcggact gcggcatcca cccctcgttc gcggcgacga
     6781 cgaacaggca cacggtgatc ccgcccgcgc cggcgagcat gttcctgacg aacttctcgt
     6841 gacccggcac gtcgaccacg ccggcctcga ggcccgacgg gagctgcacc cacgcgaagc
     6901 cgaggtcgat cgtgagcccg cgccgcttct cctcctcgaa gcggtccggg tcgatcccgg
     6961 tcagccgccg gacgagcgtc gacttcccgt ggtcgacgtg gccggcggtg cctatgacct
     7021 gcatgcccgg acgatacgcg ggtaaggacg aggggcacga cccgacccga aaggaacccc
     7081 gatgcagacg ctcgaacgac cccgcgcgca catgccggcg cgcccgcagc ccagggaacc
     7141 catctccgac ccggtcccct ctccgtcccc cgagcccggc cccgagccgc gccagcccgg
     7201 gcccgcgccg agcaccgagc ccgtgccgcc gcaggacccc gaccgggagg aagcggacac
     7261 cacgggggcc gagcgttgat cgccgcgatc ctggacatcg acgggacgct ggtcgacacg
     7321 aactaccagc acgcgctcgc gtggtaccgc gcgttcaagg cctacgacgt gatcgtgccg
     7381 gtgtggcgca tccatcgcca cgtcggcatg ggcggcgacc atctgatcac cgcgctcact
     7441 gacgaagaga ttgaggagcg cctcggcgac gacatccgcg ccgccgagaa gaagctgtac
     7501 gccgagctga tcgacgaggt cgccccgatg gagggtgcgg ccgagctcgt ctccgacctg
     7561 aaggagcgcg gtcaccgcgt cgtcctggct tcgtccgcga aggaggaaga ggtggaccac
     7621 tacctcgacc tcctcggcgt gcgcgacgcg gtcgacggct ggacgacctc cgccgacgtc
     7681 gaggcgacga agcccgagcc cgacctcgtc gaggccgcgg tcgagaagtc gggtgccggc
     7741 aaggcggtca tggtcggcga ctcgatctgg gactgcgagg ccgccgagcg cgcgggactt
     7801 ccgacgatcg gcgtcctcac cggcggtttc gcccggtccg agctggagga gcggggcgcg
     7861 gccgcagtct tcgagtccgt cgaggagcta cgcaggaacc tgaacgacac cccactccga
     7921 tagagaggag caacacatgt cgcaccatga gaagaagatc gtcaagctgc tgagcgaggc
     7981 ccacgcgaac gagctcgcgc tcgtgcagac gctcgaggcg cacaagcgcg tcgcgcccga
     8041 gggcccctac ctcgaccgca tcgaccagca cgtcgacgag acccgcaacc acgccgagcg
     8101 catgcagcgg cgtctcgacc agctcggcgc gacgaggaac ccggtcgagg ccggtgtcgc
     8161 gctcgtccac tcgttcatcc agaacacgct cgcgatggcg aaggccccgg tcgacatggt
     8221 gcgcggctcg ttcaacgtgg aggagaaggc gctcaagaac gcgcgcgacg agatcgtcac
     8281 cgaagccctc gagatcgcgc agtacgaggc catcgagcac atggcgatcg cgctgggtga
     8341 cgacgtcacc gcccgcctcg ccgccgacat ccgcgcggac gaagaggcga tgctcgccga
     8401 cctgcgggcg cagatcccga cgttggtcgc gggggtcgtg cgcgcgagcg tcccggcgga
     8461 cgagatcgtg ctcgacgcgg tgatcccgtg gccgggctac gacgacatga cggtcgacga
     8521 gatcaaggag cgcctggcgt cgtcgccgga gctcggcgac gcggtcgagg cgtacgagcg
     8581 ggcccacaag aaccgggtgt cgatcgtccg cgagctgcag gacgcctgag agcgcgaagg
     8641 aactgcggac tagttcttta ggaccagacg cttcctgccg atagagggag cgtagtcacc
     8701 accaccacca cacgacagca ccggtcgcct cggcgagcgg tccaacacga caagggcaaa
     8761 cccgtcgcaa ggcgggggcg caaagccagg ggcctcccgg cgtgagccgg gaggcggccc
     8821 agctaccgaa tggaggtcgt tcccgtgcac cgttccgcca cccgcaccct gactgtcgtc
     8881 gcactcaccg gactgctcgc cgccgcctcg ctgccggccc aggcgaagtc ccgccacaag
     8941 gagcaccgcg gcaagaaggc catgggcgag atcgtctcgt tcgacgaggc gacgatgacg
     9001 ctcgaggtcg acctgcccgg cgacgaggac ttctccggcg ccgtcgatcc cgacgccagg
     9061 gtgaagctcg accaccgcgg ctggaacaag cggggcggct cgcacggcaa cccgaccgag
     9121 ggctcgctcg acgacctcct gccgggggcc gcggttttgc ggatgaaggt cgaggacgat
     9181 ctcgtcacca agatccggat ccgcccgatt gccgaagcgc cgctgccgcc cgccgaggac
     9241 ggtgacgacg aggacgccct gccgggcgac gagaccccgg cggacgacac cccgggcgac
     9301 gactcgaccg acgattcaga agacgactca gacgacgact ccgacgacga agacgccgac
     9361 acgaacccgg acgacgagga cctcggggac ccggaggacg ccctccctcc cctgccggtg
     9421 ccccagccgt agcgtcatcc ggcccccgag ggcctggtcc gggcaaatag aaaggagcgg
     9481 ccgtcgcccc cggccgctcc ttgcggcg
//
LOCUS       DASQDO010000101         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_745269, whole genome shotgun sequence.
ACCESSION   DASQDO010000101 DASQDO010000000
VERSION     DASQDO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745269"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VEV43_03200"
     CDS             <1..888
                     /locus_tag="VEV43_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22557.1"
                     /translation="IDSASGNSSTKEDGCNLGGTENTEGIRLVDVSNRRKPVEVAFVE
                     TACGSHTNITLEHEGRGLVYVQSYPASTSGACPSAHGIISVVDITIPRAPEIVATPSV
                     APAVGCHDGAIKGELAYMACLTEGQVWDVSDPLKPEILAHIRDVPDAIWHSSAVSNDG
                     KTVAFGFESFQGGNTSCAGAARGPLGAIWFYDVSDPSAPVQKSFFSPGRMSEGICTAH
                     NFTVVPDERKRDVLVSAWYGGGVTAVDFTDPAAPEELAYYQVASGTSTWHANWYRGRV
                     WVGDGTRGLDVYEIKGLGR"
     gene            complement(891..1229)
                     /locus_tag="VEV43_03205"
     CDS             complement(891..1229)
                     /locus_tag="VEV43_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HYP22558.1"
                     /translation="MPRRDRDRLVDILEAIDRIERYTTPQSFEAHPAEIIWDAVAYNL
                     LVVGEAAAHVSDDVRARAAVDWRRIVALRNVIAHEYFDVQREMIGNTVSSDIPALRHA
                     VTGLLAGLDD"
     gene            complement(1219..1515)
                     /locus_tag="VEV43_03210"
     CDS             complement(1219..1515)
                     /locus_tag="VEV43_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYP22559.1"
                     /translation="MDASLEGLPVKEIVEIARRNGATSVSVFGSRARGDARPDSDLDL
                     LVDLAPSTTLFDLARMHVELTDLLDVGVDVVPRSGLKPRLRDEVLREAKPLDAA"
     gene            complement(1538..3352)
                     /gene="lepA"
                     /locus_tag="VEV43_03215"
     CDS             complement(1538..3352)
                     /gene="lepA"
                     /locus_tag="VEV43_03215"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HYP22560.1"
                     /translation="MDQAHQRAIAVIAHIDHGKSTLSDRLLRLTDTVSERDMRDQFLD
                     KMDLERERGITIKAAAVTMRYNAADGETYEVNLIDTPGHVDFGYEVSSSLAACDGVLL
                     LVDAAQGIEAQTLTNLYLAVDAGLEIIPVLNKIDLPAAQPDKYAEETASLIGCDPDDV
                     FRISAKTGEGVPELLEAIVTKVPPPSGDPAAPLRALVFDSVYDAYRGVIAYLRVIDGQ
                     MKVRQPIRFMIAGIDVDADEIGIMAPEATQLEMLDTGEVGYLIAGIKDVSLAKVGDTI
                     TSGTDPASEPLPGYREPKPMVFAGIYPIEGDDFPLLRDALEKLKLNDAALVYQPESSG
                     ALGFGFRCGFLGLLHMEIVRERLEREFDLSLIATAPSVGYEVEMMDGVKQVVRNPTDM
                     PDPGSIQEIYEPYVSVMIVTPSEFVGPVMQMCQERRGELKEMHYLSSERVEIRYHMPL
                     GEIIFDFFDQLKSGTKGYASLDYEPVGLYPSKLVRVDVLLNQQPVDAFSTIVHRDKAY
                     DYGKTMTERLRKLIPRQMFDVPIQAAIGSRVIARETVRAKRKDVIAKCYGGDITRKRK
                     LLEKQKAGKKRMKQIGSVEVPQNAFIEALRVDADQKST"
     gene            3423..3686
                     /gene="rpsT"
                     /locus_tag="VEV43_03220"
     CDS             3423..3686
                     /gene="rpsT"
                     /locus_tag="VEV43_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008729616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYP22561.1"
                     /translation="MANIKSQIKRNRQNELARQRNKATRSALKTRIKRFTESVDAGDS
                     AAAEEAYREAARALDKAAAKGVLHKNTASNTKSRLAKRLQRSS"
BASE COUNT          609 a         1284 c         1223 g          629 t
ORIGIN      
        1 atcgactccg cgtcgggcaa ctcgtcgacg aaggaggacg gctgcaacct cggcggcacc
       61 gagaacaccg aggggatccg gctggtcgac gtctcgaacc gccgcaagcc ggtcgaggtc
      121 gcgttcgtcg agaccgcgtg cgggagccac acgaacatca cgttggagca cgagggccgc
      181 gggctcgtat acgtccagtc gtacccggcg tcgacgagcg gcgcgtgccc gagcgcgcac
      241 gggatcatct cggtcgtcga catcacgatc ccgcgggcgc cggagatcgt cgcgacgccg
      301 agcgtcgcgc ccgcggtcgg gtgccacgac ggcgcgatca agggcgagct cgcatacatg
      361 gcgtgcctca ccgagggcca ggtgtgggac gtgagcgacc cgctgaagcc ggagatcctc
      421 gcccacatcc gggacgtgcc ggacgcgatc tggcactcga gcgcggtctc gaacgacggc
      481 aagaccgtgg cgttcgggtt cgagtcgttc caggggggca acacgtcgtg cgccggtgcg
      541 gcccgcggcc ccctgggcgc gatctggttc tacgacgtgt cggatccctc cgctcccgtt
      601 cagaagagct tcttctcgcc ggggaggatg agcgagggca tctgcacggc gcacaacttc
      661 acggtggtcc cggacgagcg caagcgtgac gtgctggtct cggcctggta cggaggcggc
      721 gtgacggcgg tggacttcac ggacccggca gcgcccgaag agcttgcgta ctaccaggta
      781 gcgagcggga cgagcacctg gcacgcgaac tggtaccggg gccgggtctg ggtaggcgac
      841 ggcacgaggg gcctggacgt ctacgagata aagggcctag gccgctagcc ctagtcgtcg
      901 aggcccgcga gcaaccccgt cacggcgtgg cgtagcgcgg ggatgtccga gctcaccgtg
      961 ttgccgatca tctcgcgctg gacgtcgaag tactcgtgag cgatgacgtt cctcagggcg
     1021 acgatgcggc gccagtcgac ggcggctcgt gcccgcacgt cgtccgaaac gtgggccgca
     1081 gcttcgccta cgacgaggag gttgtacgcg actgcatccc aaatgatctc tgccgggtgc
     1141 gcctcgaacg attgcggcgt cgtgtaacgc tcgatccggt cgatcgcttc gaggatgtcg
     1201 accagccggt ctcgatctct acgcggcatc cagcggcttc gcctcgcgca ggacttcgtc
     1261 ccgcaaccgt ggtttcagcc ccgaacgagg cactacgtcg actcccacgt ccagaaggtc
     1321 ggtcaattcg acgtgcatcc gagcaaggtc gaacaacgtc gtgctcggcg cgaggtcgac
     1381 cagcaggtcc aggtcgctgt ccggcctcgc gtccccccgg gcccgcgagc cgaacacgct
     1441 caccgacgtc gcaccgttgc ggcgggcgat ctcgacgatc tccttgaccg gaaggccttc
     1501 cagcgacgcg tccatgcccc cgatgatacg acggccccta cgtgctcttc tggtccgcgt
     1561 ccaccctcag cgcctctatg aacgcgttct gcggcacctc taccgaacct atctgcttca
     1621 tcctcttctt cccggccttc tgcttctcga gcagcttccg cttcctcgtt atgtcaccgc
     1681 cgtagcactt cgcgatcacg tccttgcgct tggcccggac ggtctcgcgc gctatcaccc
     1741 gtgacccgat cgcggcctgg atcgggacgt cgaacatctg ccgagggatc agcttgcgca
     1801 gcctctcggt catcgtcttg ccgtagtcgt acgccttgtc gcgatgcacg atcgtcgaga
     1861 acgcgtcgac cggctgctgg ttcaacagca cgtcgacccg cacgagcttc gacgggtaca
     1921 gccccaccgg ctcgtagtcc agcgacgcgt accccttcgt cccgctcttc agctggtcga
     1981 agaagtcgaa gatgatctcg ccgagcggca tgtggtagcg gatctcgacc cgctcggacg
     2041 agaggtagtg catctccttg agctcgccgc gccgctcctg gcacatctgc atgaccggcc
     2101 cgacgaactc agacggcgtc acgatcatca ccgagacgta cggctcgtag atctcctgga
     2161 tcgagccggg gtcgggcatg tcggtcgggt tgcggacgac ctgcttgacc ccgtccatca
     2221 tctctacctc gtatccgacc gacggcgcgg tcgcgatcag cgacaggtcg aactcgcgct
     2281 cgagccgctc gcgcacgatc tccatgtgca acagcccgag gaacccgcag cggaagccga
     2341 accccaacgc tcccgacgac tccggctggt agacgagcgc ggcgtcgttg agcttcagct
     2401 tctcgagcgc gtcgcgcagc agcgggaagt cgtcgccctc gatcgggtag atgccggcga
     2461 acaccatcgg cttcggctcg cggtagcccg gcaggggctc ggaagcggga tcggtccccg
     2521 acgtgatcgt gtcgccgacc ttcgccaggc tcacgtcctt gatccctgcg atcagatagc
     2581 cgacctcgcc cgtgtcgagc atctcgagct gcgtcgcctc gggcgccatg atccctatct
     2641 cgtccgcgtc gacgtcgatg cccgcgatca tgaagcggat cggctggcgc accttcatct
     2701 ggccgtcgat cacccgcagg tacgcgatca cgccgcggta cgcgtcgtac acggagtcga
     2761 agacgagcgc gcgcagcggc gcggcggggt cgcccgacgg tggcggcacc ttggtgacga
     2821 tcgcctcgag gagctccggc acgccctcgc ccgtcttcgc cgagatccgg aagacgtcgt
     2881 cgggatcgca gccgatcagg ctcgcggtct cctccgcgta cttgtcgggc tgcgccgcgg
     2941 ggaggtcgat cttgttcagc acggggatga tctcgagccc ggcgtcgacg gcgaggtaga
     3001 ggttcgtcag ggtctgcgcc tcgatccctt gcgcggcgtc gacgagcagc aggaccccgt
     3061 cgcacgcggc gagcgagctc gacacctcgt agccgaagtc gacgtggccg ggcgtgtcga
     3121 tgaggttgac ctcgtacgtc tcgccgtcgg ccgcgttgta gcgcatcgtc acggcggccg
     3181 ccttgatcgt gatcccgcgc tcgcgctcga ggtccatctt gtcgaggaac tggtcgcgca
     3241 tgtcccgctc cgagacggtg tcggtcagcc gcaggagacg gtcggagagc gtgctcttcc
     3301 cgtggtcgat gtgggcgatg acggcgatcg ctcgctggtg ggcctggtcc atacgagccc
     3361 caagttaccg gcaagtttgg tactctctgc gcctatccca ccaaggaaca ggtgttttcg
     3421 gcatggccaa catcaagagt cagataaaga ggaatcgcca gaacgagctc gcccgccagc
     3481 gcaacaaggc gacgcgctcg gcccttaaga cgcgcatcaa gcgcttcacc gagtcggtcg
     3541 acgccggcga ctccgccgcc gccgaggagg cgtaccgcga ggccgcacgg gccctcgaca
     3601 aggccgcggc caagggcgtc ctccacaaga acacggcttc gaacacgaag tcgcgcctgg
     3661 cgaagcgcct ccagcgctct agctgatcag cgactcctga ccgctgacga tctgaactac
     3721 cgcgcgctcg agcgccgcct ccggc
//
LOCUS       DASQDO010000102         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_867451, whole genome shotgun sequence.
ACCESSION   DASQDO010000102 DASQDO010000000
VERSION     DASQDO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867451"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VEV43_03225"
     CDS             <1..1380
                     /locus_tag="VEV43_03225"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYP22562.1"
                     /translation="GEISPEEILHALHDGIDNATFFPVLVGSATKLIGVDRLADVIVA
                     AGPSPLDRPPAEGTNGEAREAKPDAPLSAFVFKTMTDPYVGRVSYFRVVSGVLKGDTT
                     LHNSTQNLDERVGHLFTMRGKTQEPVQELVAGDIGAAAKLAHTVTGDTIADKANPVVL
                     PPIEPPEPLLPKAIVPKTKGDEDKLMIGLHKLIEEDPALKLERNDETHQTILWGMGDA
                     HLDVTMERLKRKFGVEVGEEPFKVPYRSTLKGKADALGRHVKQSGGHGQYGICNVRVE
                     PLPRGEGFVFDDKIFGGSIPSQFIPSVEKGVRAAMEKGAGTGFPMIDIKVELYDGKYH
                     TVDSSDMAFQTAGSLAMRDAIDKAGVTLLEPVWALDVMVPEEFTGDIMGDLQKRRGIP
                     EGIDTVGGGRQIVKAKVPFGEVTHYATDLRSMTGGKGTFSWSFSHYQEVPGDVAKKIL
                     DATKEEAHA"
     gene            1394..1918
                     /locus_tag="VEV43_03230"
     CDS             1394..1918
                     /locus_tag="VEV43_03230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22563.1"
                     /translation="MDPLVAGDAEELLDVMLRNRAFLEPYEPARPESDFTIQGVRAAI
                     AELGAARDAGAAYPFCIRAEGRIVGRITLSQVFRRAFLNCYLGYFVGEEHNGRGYATG
                     AVRLAVRYAFDELGLHRVQANVMTTNPRSARVLVKAGFREEGRALRYLQIAGRWEDHD
                     LYAITVEDVAPGAI"
     gene            2014..2595
                     /locus_tag="VEV43_03235"
     CDS             2014..2595
                     /locus_tag="VEV43_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22564.1"
                     /translation="MPASRIAALGLTLLLAGGLVACGGDDGGDGGDGGGGGSSESISA
                     EDYAAEVCGAMQDWITGIQEGTQDLGAGLSAGDAEGGKKVLVDLFTKMSESTGELVTA
                     VEDAGVPDVEGGEEARETLVSAFQDVQGILEDAQADAEALPSDPQEFQEGANELGTTL
                     QEALTSVGESLEEPPSDELKTAFENEESCNSVP"
     gene            complement(2549..4060)
                     /gene="lnt"
                     /locus_tag="VEV43_03240"
     CDS             complement(2549..4060)
                     /gene="lnt"
                     /locus_tag="VEV43_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYP22565.1"
                     /translation="MSSRRRSAAAVGAVAVSGAALTLAFPEPDLAPLAWVCLAPLLAV
                     LKGAGAKRGFLFGAVFGLCYFGTLVHWVAIVGYLAWFVLILLQAAYVGAFGALWGALS
                     KRAGPLAAIVMPALLWVAVVEYLRAVTPVVGFTWGQLAQSQHDVPFVLRSASVGGAWL
                     VALQLVAVNALIAAAVHAGARRDLRAVAVRGVAAAAVLLAAAPFPGPRADGEAVDVAI
                     VQGNIPEHQAPSYEKDLVILDNHARLTAEVPPEADLVLWPESSVGIDPARDPQAGAIL
                     REAARGAERPMIVGGTLARDDGKYQVMAYLVSPDGEITDAYQKTHLVPFGEYVPARDL
                     LGWLPAIEQVPLDAVPGDEPVVFDVAGGRVAPVISFEADFGSLASGRVRAGGRLLVVL
                     TNTSTWRHSWASAQHVAMSEVRAAEAGVYVAHGALTGISAFVDPSGHVLDRSELWEPD
                     LLVQELRFATGASFYARTGDWAAWLSVAGAALYAVAALLRGRSYRTPRSRTPS"
     gene            complement(4041..4595)
                     /locus_tag="VEV43_03245"
     CDS             complement(4041..4595)
                     /locus_tag="VEV43_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22566.1"
                     /translation="MDNEGGPRPASEDAGFRLETERLILRPFALDDLDELAPILGDAE
                     TMQYYPAPFTRERSRQWIVWNLANYRDHGHGLWVLESRETGELLGDCGLIPQTIDGRK
                     EVEVGWHVKKTHWSRGLATEAGAACRDHAFGELGLTRLISLIRPENVPSRRVAEKLGM
                     TIEKEISHGPGSGMPHFVYVLEKT"
BASE COUNT          749 a         1614 c         1654 g          605 t
ORIGIN      
        1 ggcgagatct cacccgaaga gattctccac gcgctgcacg acggcatcga caacgccacg
       61 ttcttccccg tgctggtggg ctcggcgacg aagctcatcg gcgtcgaccg tctcgccgac
      121 gtcatagtcg ccgccggacc gtcgccgctg gaccggcccc cggccgaggg aacgaacggc
      181 gaggcccgcg aggccaagcc ggacgcgccg ctgtcggcgt tcgtgttcaa gacgatgacc
      241 gacccctacg tgggccgcgt cagctacttc cgcgtggtct ccggcgtgct caagggcgac
      301 accacgttgc acaactcgac gcagaacctc gacgagcgcg tcggccacct gttcacgatg
      361 cgcggcaaga cccaggagcc cgtccaggag ctcgtcgcgg gcgacatcgg cgcggccgcc
      421 aagctcgcgc acaccgtcac cggcgacacg atcgccgaca aggcgaaccc ggtcgtgctc
      481 ccgccgatcg agccgcccga gcccttgctt ccgaaggcca tcgtcccgaa gaccaagggc
      541 gacgaggaca agctgatgat cggcctccac aagctcatcg aggaggaccc ggcgctcaag
      601 ctcgagcgca acgacgagac gcaccagacg atcctgtggg gcatgggcga cgcgcacctc
      661 gacgtgacga tggagcggct caagcgcaag ttcggcgtcg aggtcggcga ggagccgttc
      721 aaggtcccct accgctcgac gctcaagggc aaggccgacg cgctcggacg gcacgtcaag
      781 cagtccggcg gccacgggca gtacggcatc tgcaacgtgc gcgtcgagcc gctgccgcgc
      841 ggcgagggct tcgtcttcga cgacaagatc ttcggcggct cgatccccag ccagttcatc
      901 ccgtcggtcg agaagggcgt acgggccgcg atggagaagg gggccgggac cggcttcccg
      961 atgatcgaca tcaaggtcga gctctacgac ggcaagtacc acaccgtcga cagctcggac
     1021 atggcgttcc agaccgcggg gagcctcgcg atgcgcgacg cgatcgacaa ggccggggtg
     1081 acgctgctcg agccggtctg ggccctcgac gtcatggtgc ccgaggagtt caccggcgac
     1141 atcatgggcg acctccagaa gcggcgcggg atccccgagg gcatcgacac cgtcggcggt
     1201 ggccgtcaga tcgtcaaggc caaggtcccg ttcggcgagg tcacgcacta cgcgaccgac
     1261 ctccggtcga tgaccggggg caaggggacg ttctcgtggt cgttctcgca ctaccaggag
     1321 gtgccgggcg acgtggccaa gaagatcctc gacgccacga aggaagaagc ccacgcctga
     1381 gcagagcacg cgcatcgacc cgctcgtagc gggcgacgcc gaggagcttc tggacgtgat
     1441 gctccggaac cgcgcgttcc tcgagcccta cgagccggcg cggcccgagt ccgacttcac
     1501 gatccagggg gtccgggcgg cgatcgccga gctcggcgcc gcgcgcgacg cgggcgcggc
     1561 ctacccgttc tgcatccgcg cggagggccg catcgtcggg cggatcacgc tgtctcaggt
     1621 cttccgcagg gcgttcctca actgctacct cggctacttc gtcggcgagg agcacaacgg
     1681 gcgcggctac gcgacggggg ccgtccggct cgcggtccgc tacgcgttcg acgagctggg
     1741 cctccatcgg gtccaggcga acgtgatgac gacgaacccg cgctcggccc gggtgctcgt
     1801 gaaggcgggg ttccgcgagg agggccgcgc cctgcgctat ctccagatcg cgggccgctg
     1861 ggaggaccac gacctgtacg cgatcacggt ggaggacgtg gcgcccgggg cgatctgagc
     1921 gctttgtcac ggcttcgatg tctcgaagaa acgatccccg cgtcgtatcg tcatcagctg
     1981 gagatctcgg cacccccgga caaggagacc tgcatgcccg catcgaggat cgctgcgctc
     2041 ggactcaccc tgctcctcgc gggcgggctc gtcgcctgtg gtggcgacga cggtggtgac
     2101 ggtggtgacg gtggcggtgg cggctcgtcg gagagcatct cggccgagga ctacgcggcc
     2161 gaggtctgcg gcgcgatgca ggactggatc accggcatcc aggaggggac gcaggacctg
     2221 ggcgccggcc tctccgccgg tgacgccgaa ggcggcaaga aggtgctcgt ggacctcttc
     2281 accaagatga gcgagtcgac cggcgagctc gtgaccgcgg tcgaagacgc gggcgtcccc
     2341 gacgtcgagg gcggcgagga ggcgcgcgag accctcgtct ccgcgttcca ggacgtccag
     2401 gggatcctcg aggacgcgca ggccgacgca gaggccctgc cctcggaccc gcaggagttc
     2461 caggaaggtg ccaacgagct ggggacgacc ctccaggagg ccctgacgag cgtcggcgag
     2521 tcgctcgagg agccgccgtc cgacgagctc aagacggcgt tcgagaacga ggagtcctgt
     2581 aactccgtcc cctaaggagc gcggcgaccg cgtatagcgc ggcccccgcg acggaaagcc
     2641 acgcggccca gtcaccggtg cgggcataga acgacgcgcc ggtcgcgaag cggagctcct
     2701 gcacgagcag gtccggctcc cagagctcgg agcggtcgag cacgtggccc gacgggtcga
     2761 cgaacgccga tatcccggtg agcgcgccgt gcgcgacgta gacgccggcc tcggcggcgc
     2821 gcacctcgct catcgcgacg tgctgcgccg acgcccacga gtggcgccac gtcgaggtgt
     2881 tcgtgagcac gacgagcagg cggccgccgg cacggacgcg cccggatgcg agcgagccga
     2941 agtcggcctc gaacgagatc accggtgcga cccggccccc cgcgacgtcg aagaccacgg
     3001 gctcgtcgcc ggggaccgcg tcgagcggca cctgctcgat cgccgggagc cagccgagca
     3061 ggtcgcgcgc ggggacgtac tcgccgaagg ggacgaggtg ggtcttctgg tacgcgtcgg
     3121 tgatctctcc gtcgggtgag acgaggtacg ccatcacctg gtacttgccg tcgtcgcggg
     3181 cgagcgtccc gccgacgatc atcggccgct ccgctcccct ggcggcctcc cgcaggatcg
     3241 cgccggcctg cgggtcgcgg gccgggtcga tgccgaccga gctctccggc cacaggacga
     3301 ggtcggcctc gggcgggacc tccgcggtga ggcgggcgtg gttgtcgagg atcacgaggt
     3361 ccttctcgta cgagggggcc tggtgctcgg ggatgttgcc ctggacgatc gcgacgtcga
     3421 cggcctcgcc gtccgcgcgg gggccgggga acggcgccgc ggcgagcagg acggcggccg
     3481 ctgcgacgcc gcgaaccgcg acggcgcgga ggtcccggcg cgcgcccgcg tgcaccgccg
     3541 cggcgatcag cgcgttgacg gccacgagct gaagcgcgac gagccacgcc ccgcccaccg
     3601 aggcggagcg caacacgaac gggacgtcgt gctggctctg cgcgagctgc ccccacgtga
     3661 acccgaccac gggcgtgacc gctcgcaggt actcgacgac cgcgacccac agcagtgccg
     3721 gcatcacgat cgccgcgagg gggccggccc gtttcgacag cgctccccac agcgcgccga
     3781 acgcgccgac gtaggccgct tggagcagga tgagcacgaa ccacgcgagg tagccgacga
     3841 tcgcgaccca gtgcacgagc gtgccgaagt agcagaggcc gaagacggct ccgaacagga
     3901 acccgcgctt cgctccggct cccttcagga cggccagcag cggcgcgagg cacacccatg
     3961 cgagcggcgc gaggtcgggc tcggggaacg cgagcgtgag ggcggcgccc gagacggcga
     4021 ccgctccgac cgccgccgcg ctacgtcttc tcgaggacat agacgaagtg cggcatgccc
     4081 gatcccggac cgtggctgat ctccttctcg atcgtcatgc cgagcttctc cgcgacgcgc
     4141 cgcgacggga cgttctccgg acggatcagc gagatcagcc gggtgagccc gagctcaccg
     4201 aacgcgtggt cgcggcacgc ggcccccgct tcggtcgcga gcccccggga ccagtgcgtc
     4261 ttcttcacgt gccagccgac ctcgacctcc ttgcggccgt cgatcgtctg cgggatcagc
     4321 ccgcagtcgc cgaggagctc gccggtctcc ctggactcga gcacccacag cccgtggccg
     4381 tggtcgcggt agttggcgag gttccacacg atccactggc gcgatctctc gcgggtgaag
     4441 ggggccgggt agtactgcat ggtctcggcg tcgccgagga tcggcgccag ctcgtcgagg
     4501 tcgtcgagcg cgaacgggcg caggatcagc cgctcggtct cgagcctgaa gccggcgtcc
     4561 tcggaggccg gccgaggccc cccttcgtta tccaagccgc tcgaacaacg gcttcagctt
     4621 cg
//
LOCUS       DASQDO010000103         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_316803, whole genome shotgun sequence.
ACCESSION   DASQDO010000103 DASQDO010000000
VERSION     DASQDO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316803"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VEV43_03250"
     CDS             <1..1507
                     /locus_tag="VEV43_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22567.1"
                     /translation="IPQAMATGTSGIWTVGWQGDMPVGGEGHPVRGAIERVDPVTLER
                     TGHIPREDSAPYDVAVGAVDGREVAWVVDAGRHELLRVDAATMEISAVYETPRVPVSV
                     LVHGRFVYVTSSKDHVVHRMDVETEETTTFEVPECANEVVVGGGALWVADFCGDALHR
                     IDEDGSEEIVSIDVAEPNALQYAEGLVWVNSRSGVVRVDPGSNTVAGDSVYVSEFNQG
                     LLYAGGTMWVYGMEGVYRLGEDVPPATPTPTPPPTPDPRGKALPPGVTRVPVERDPAA
                     LATGDGSLWAGMFEIVRVDAATGEVRAEIDPGGFVHSLDFDEAAGMLWAFVEVSEKET
                     NAVVAIDPATDEVVLGPVLLDKPGVYGQAMAAHDGVAWVAGDPTLSRIDLTTGDVQEI
                     DMGRHFGGPDETSGFNVAATDDAVFVTPVSGTVLRVDPETFEIEKVAELGWNVVGLVT
                     DGETVWLLQTSGDTSEILLWTLDGATGEALGEPVVVGKYGSEHVAQHDGTV"
     gene            1508..1690
                     /locus_tag="VEV43_03255"
     CDS             1508..1690
                     /locus_tag="VEV43_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22568.1"
                     /translation="MAQAGMKGDAISIKAYAGGRGEPVRSLEVPGTVFVQAAAAGPEG
                     FWITTGHDFVYRVGAE"
     gene            1732..1803
                     /locus_tag="VEV43_03260"
     tRNA            1732..1803
                     /locus_tag="VEV43_03260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1764..1766,aa:Gly,seq:gcc)
     gene            1878..>2022
                     /locus_tag="VEV43_03265"
     CDS             1878..>2022
                     /locus_tag="VEV43_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP22569.1"
                     /translation="MPQVRRFDHVGITVADLDIVTAFFVALGLEVEGRTFVEGEFLDT
                     VCGI"
BASE COUNT          302 a          623 c          788 g          309 t
ORIGIN      
        1 gatcccgcag gcgatggcga cggggacctc gggcatctgg accgtagggt ggcagggaga
       61 catgcccgtc ggcggtgagg ggcacccggt gcgcggcgcg atcgagcgcg tcgacccggt
      121 gacgctcgag cgcaccggcc acatcccgcg cgaggactcg gccccctacg acgtcgcggt
      181 cggagccgtc gacggccggg aggttgcatg ggtcgtcgac gcgggtcgcc acgagctcct
      241 ccgggtcgac gcggcgacga tggagatctc ggccgtctac gagacgccgc gcgtgccggt
      301 gtcggtcctc gtgcacggac gcttcgttta cgtgacgagc agcaaggacc acgtggtcca
      361 ccgcatggac gtcgagaccg aggagacgac gacgttcgag gtcccggagt gcgcgaacga
      421 ggtggtcgtc ggaggcggcg cgctgtgggt agcggacttc tgcggcgacg cgctgcaccg
      481 gatcgacgag gacgggtccg aggagatcgt gtcgatcgac gtcgccgagc cgaacgcgct
      541 ccagtacgcg gaagggctgg tctgggtcaa ctcgcggtcc ggggtcgtgc gcgtcgaccc
      601 ggggtcgaac acggtcgccg gcgactccgt ctacgtgagc gagttcaacc aggggctgct
      661 ctacgccggg ggcaccatgt gggtgtacgg gatggagggc gtctatcgcc tcggcgagga
      721 cgtcccgccg gcgactccga cgccgactcc gcctccgacg cccgaccccc gcggcaaggc
      781 gctgcctccc ggcgtgacgc gggtgccggt cgagcgggac ccggccgcgc tcgccaccgg
      841 cgacgggtcg ctgtgggcgg ggatgttcga gatcgtccgc gtcgatgcgg cgacgggcga
      901 ggttcgggcc gagatcgatc ccggtgggtt cgttcactcc ctggacttcg acgaggccgc
      961 gggcatgttg tgggcattcg tcgaggtctc cgagaaggag acgaacgcgg tcgtggcgat
     1021 cgaccctgca accgacgagg tcgtgctcgg cccggtcctg ctcgacaagc ccggcgtgta
     1081 cggccaggcg atggcggcgc acgacggcgt cgcatgggtg gcgggcgacc cgacgctgtc
     1141 acggatcgac ctgacgaccg gcgacgtgca ggagatcgac atgggccggc acttcggggg
     1201 gccggacgag acgagcggct tcaacgtggc cgcgaccgac gacgccgtgt tcgtgacgcc
     1261 ggtcagtggc acggtccttc gtgtcgaccc ggagacgttc gagatcgaga aggtcgccga
     1321 gctcggctgg aacgtcgtcg ggctcgtgac cgacggcgag acggtctggc tcctgcagac
     1381 gagcggggac acctcggaga tcctgctgtg gactctcgac ggagcgacgg gtgaggctct
     1441 cggcgagccc gtcgtggtcg ggaagtacgg ctccgagcat gtcgcgcagc acgacgggac
     1501 cgtgtaggtg gcgcaggccg ggatgaaggg cgacgcgata tcgatcaagg cgtacgccgg
     1561 gggccggggc gagccggtgc gctcgctgga ggtcccgggg acggtcttcg tccaagccgc
     1621 agcggccggg cccgagggct tctggataac gacgggtcac gacttcgtgt accgcgtggg
     1681 ggccgagtag cccggcccgg gcccccggcg aggccatata atgagggggt tgcgggcgtg
     1741 gctcagtggt agagcatcac cttgccaagg tgagggtcgc cagttcgaat ctggtcgccc
     1801 gctcggagta gaaatgcagg tcagggggct tattcggccc cctgtctcgc gtcgggcgca
     1861 actttggagg acggctcatg ccacaggtac gacgcttcga ccacgtcggg atcacggtcg
     1921 cggatctcga tatcgtgacc gccttcttcg tggcactggg cctggaggtc gagggcagga
     1981 cgttcgtcga gggcgagttc ttggacacgg tttgcggcat tc
//
LOCUS       DASQDO010000104         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1051356, whole genome shotgun sequence.
ACCESSION   DASQDO010000104 DASQDO010000000
VERSION     DASQDO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051356"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VEV43_03270"
     CDS             <1..519
                     /locus_tag="VEV43_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22570.1"
                     /translation="VRVLVVPERDAAEVVNEIRGMVWAHLGVDLDVHAVEVMKATGTD
                     APNGRPKRRKLSSLSTKRSGDRFTAQVILELDGDLLIGEDDSPAGRRFEHRSVAEAVL
                     DAVRRLSPDPLELGSADFVEMGDCRIAAVVVERPDELLLGSAVVTFDEHDAIARATLD
                     AVNRLLVASSHT"
     gene            527..1729
                     /locus_tag="VEV43_03275"
     CDS             527..1729
                     /locus_tag="VEV43_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP22571.1"
                     /translation="MSDFVAGLDPRWLAALAVVAYFGGAAWLLGLNLLLATRKTDERL
                     LRAGFVRRWWRWYRSLWLRTGLTFAGWPHVVDVLLRTKRVSVATTPAEVVWEEGRAAL
                     YRYRTTVRHREPVLVVHAVVSKPWILDLTPESSFVAALAAEGFDVFLLDWGDPEWPDA
                     SRGLTDYTQTLMRAEKIALTLAGSDRLHLVGYCFGGTLCLARAAARSHAHVGSIALLA
                     APVDFTVPAGLHPLMTHRFFKPVYLLDAEGMVPAPAVRESFHVLRPQAIRTALRAWKG
                     RRDRSFRAAYDPLARWVWEHRALPGRMFFDLVDLFRTNALVAGTLELAGEVARLPDVR
                     APILAIIPERDHITPSGSSHALTTQPGLDVTVVNAAAGHVSMIAGPAARTTTWPEVAS
                     WLERHQEP"
     gene            1821..>2173
                     /locus_tag="VEV43_03280"
     CDS             1821..>2173
                     /locus_tag="VEV43_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22572.1"
                     /translation="MRRICSAIVGALLVVVAAVPAGAGGALLDFDREFYVPGDVVHAS
                     SGVWLKSARGRLEHGPYFAYVSRYTGLDSMPNPAPVDAQRVAEARIEPRDHRYGDVYV
                     EFPLPDLEPGTYWVT"
BASE COUNT          273 a          789 c          777 g          334 t
ORIGIN      
        1 gtccgggtcc tcgtcgtgcc ggagcgtgac gccgcggagg tcgtcaacga gatccgcggc
       61 atggtctggg cgcacctcgg cgtggacctc gacgtccacg cggtcgaggt catgaaggcc
      121 accgggacgg acgcgcccaa cggccgtccc aagcgccgga agctgtcgag cctgtcgacg
      181 aagcgttccg gcgacaggtt caccgcgcag gtcatcctcg agctcgacgg cgacctgctg
      241 atcggcgagg acgactcgcc tgcgggccgc cggttcgagc accgctcggt tgcggaagca
      301 gtactcgacg cggtccggcg actgtcgccc gacccgctcg agctcggctc cgccgacttc
      361 gtcgagatgg gcgactgccg catcgccgcg gtcgtcgtcg agcgccccga cgagctcctg
      421 ctcgggtcgg ccgtcgtcac gttcgacgag cacgacgcga tcgcacgcgc gaccctcgac
      481 gcggtcaacc gcctcctggt cgcttcgtcg cacacctagc gcctcggtga gcgacttcgt
      541 cgccggcctc gacccacgct ggctcgcggc cctggccgtc gtcgcctact tcggcggtgc
      601 cgcgtggctg ctcgggctca acctcttgct cgccacgcgt aagaccgacg aacgcctgct
      661 gcgcgccggc ttcgtacggc ggtggtggcg gtggtaccgg tcgctgtggc tgcgcacggg
      721 cctgacgttc gccgggtggc cgcacgtcgt cgacgtcctc ctccggacca agagggtgtc
      781 ggtcgcgacg acgccggccg aggtcgtgtg ggaggagggc cgcgccgcgc tgtaccgcta
      841 ccggacgact gtccggcacc gcgagcccgt gctcgtcgtc cacgcggtcg tctcgaagcc
      901 gtggatcctc gacctcacgc cggagagcag cttcgtcgcc gcgctggcgg ccgaggggtt
      961 cgacgtcttc ctcctcgact ggggagaccc ggagtggccg gacgcgtcgc gagggctcac
     1021 cgactacacg cagacgctga tgagggccga gaagatcgcg ctgacgcttg ccggtagcga
     1081 ccggctccac ctcgtcggct actgcttcgg tgggacgctg tgcctcgccc gcgcggcggc
     1141 gcgctcgcac gcgcacgtcg ggtcgatcgc gctgctcgcc gcgccggtcg acttcaccgt
     1201 tccggccggg ctccatccgc tcatgaccca ccgcttcttc aagccggtct acctcctcga
     1261 cgccgagggc atggtcccgg cccccgcggt gcgcgagagc ttccacgtgc tgcgcccgca
     1321 ggcgatcagg acggcccttc gggcgtggaa aggcagacgc gaccgttcgt tccgcgcggc
     1381 gtacgacccg ctcgcgcgct gggtctggga gcaccgggca ctgcccgggc ggatgttctt
     1441 cgacctcgtc gacctgttcc ggacgaacgc gctcgtggcg gggacgctcg agctcgccgg
     1501 cgaggtcgcg cgcctccccg acgtgcgagc gccgatcctg gcgatcatcc cggagcgcga
     1561 ccacatcacc ccgagcggat ccagccacgc cctcactacc cagcccggcc tcgacgtgac
     1621 ggtcgtgaac gcggcggccg ggcacgtctc gatgatcgcg gggccggcgg cccggacgac
     1681 gacgtggccc gaggtcgcgt cgtggctcga acggcaccaa gagccctgaa gggccgcctc
     1741 ggcgggcctc ccgcctagat agaacttccg gcccccgcgc gcagtccgtc ttcgtgacgg
     1801 tcgagagcgg gaggtgcgcg gtgcggagga tctgttcggc gatcgtgggg gccttgctcg
     1861 tcgtcgtggc ggccgtcccg gcgggggccg ggggtgcgct gctggacttc gaccgggagt
     1921 tctacgtccc cggcgacgtc gtgcacgcgt cgtcgggcgt gtggctgaag tcggcgaggg
     1981 gacgcctcga gcacggcccg tacttcgcct acgtctcgcg gtacacgggg ctggattcga
     2041 tgccgaaccc ggcgccggtc gacgcgcagc gcgtcgccga ggcgcgcatc gagccacgcg
     2101 accacaggta cggcgacgtg tacgtcgagt tcccgctccc ggatctcgag ccggggacgt
     2161 actgggtcac gat
//
LOCUS       DASQDO010000105         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_316911, whole genome shotgun sequence.
ACCESSION   DASQDO010000105 DASQDO010000000
VERSION     DASQDO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316911"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VEV43_03285"
     CDS             complement(<1..1462)
                     /locus_tag="VEV43_03285"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYP22573.1"
                     /translation="MRLSLRWKVSAGFLILLGLIGILAWVTIAQFTSLRSVQRKVFND
                     AVPGLVAVDEIVRSYTAQSASVGLYLTAETGALEQYRSEVGNAETWEQRAERLFTDPA
                     ERVLLEDLTEAGDEFQEVVDRVVALASDGKRSQAIRILRDEGTPAIASIGSLGRQLAI
                     EQDRVVAVTENEVDQRSNQTIVILVAVSIGALLAGVALAIALPRRLAFNLGQLVDAAR
                     AIERGDLNQELDIRSGDEVEELAQRFIEMQSGLAKLQQLALQDRELEIAASIQQKLLQ
                     RKLPEIDGVRVVPLQRQANRVGGDWYDVDAGGRWLTVAIGDASGKGIAAALMATVMLS
                     SLRAERSRGAGPKRVIEQANLALLEATETGAFTTCVYATVDIVTGEVRWLNMGHPAPF
                     LLHAPDPARDGDPPGYYVEAEGPRNRPLGWDENPGLNQTTATLAPGDRLILFTDGFLE
                     AKSPDGEVFGEHRLEASLLELASLPSDTLAGQVVEDV"
     gene            1532..2407
                     /locus_tag="VEV43_03290"
     CDS             1532..2407
                     /locus_tag="VEV43_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYP22574.1"
                     /translation="MPPWGRLLASGAALGAAGAAYALAEARAFRLQEHRLPVRPGAPR
                     TTVLHVSDTHMQERDRLKAQWLSRLPELLEITPDVVVATGDLIEDPTGIDPLVEALSG
                     LQARYGKWYVLGSHDYFHSTFPGFFKYFSGNREVVRAKPADTPRLEAGLKETGWVALT
                     NRSETVDTPGGRIRLSGVDDPYIRRDDTRHLGREQGDALAIGVMHAPEVVSEYALHGY
                     DLALAGHTHGGQVRVPFAGAVVTNSDLPCALAAGPTRVGSSWLHVSPGVAQGKFVPLR
                     FNCPPEVTLLTLEPS"
     gene            2416..4071
                     /locus_tag="VEV43_03295"
     CDS             2416..4071
                     /locus_tag="VEV43_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP22575.1"
                     /translation="MHLVSGSMRALRLLLVAVLLAALPPLTARAAPRPPPQNASVPGQ
                     VTVVTVNAKQNRILGLARFLALFELPKALRFRPEAFNGGFRGAVTAPDVIIVQEVRPS
                     NLEILVRLLRQRFPYRYEALEPAGAAAAFVVNTDTVTPQGEVTTWDDVCTTEETPTDN
                     RATRDYPIGRFVEVATNAPFVVAGMHMAKRYDTTGQPNCVPRNMERFKEEVAGETAPV
                     IFGGDFNRRAVLDPYECDPEERGTPNPWYEILTAPQDGSRVFVDAVRQWHRGHGTSMA
                     TEWTHEQRQARVLCDETTRFKRSRIDYLFAAGAAVAEAHSDHPGWAGAEAGVRLGTPY
                     SDHRYVWGRFVVSGPPRPLQPAVTPGADGRIDVTWGAVEGAAGYVVYRALRGHAYTVL
                     AQVGADVTTFGDVFTEHEQSYRYAIAAVGADGGIGQESRPAFGIADKKGPHVVRVTPS
                     RNATAIDPGVTILIRFDERVASGSVAADTVRLWYGRRRIPGITTMAGARLLQLDPAGR
                     LDKGKTYRVVVDPVRDVLGNAGPREAWRFTTVEPPPPPPKRRR"
BASE COUNT          608 a         1526 c         1485 g          575 t
ORIGIN      
        1 ccacgtcttc cacgacctgc ccggcgaggg tgtcggaggg cagcgaggcg agctcgagga
       61 gcgacgcctc caggcggtgc tcgccgaaga cctcgccgtc gggggacttc gcctcgagga
      121 acccgtcggt gaacaggatc aggcggtcgc cgggggcgag cgtggcggtc gtctggttca
      181 gtccggggtt ctcgtcccac ccgagcggcc ggttgcgcgg cccctctgcc tcgacgtagt
      241 agccgggcgg gtcgccgtcc cgagcggggt cgggggcgtg caggaggaag ggggccgggt
      301 ggcccatgtt cagccagcgc acctcgccgg tgacgatgtc gacggtcgcg tagacgcagg
      361 tcgtgaaggc cccggtctcg gtcgcctcga gcagcgcgag gttcgcctgc tcgatcacgc
      421 gcttggggcc ggcgccgcgg ctgcgctccg cccgcagcga cgagagcatg accgtcgcca
      481 tgagggccgc ggcgatcccc ttgccggacg cgtcgccgat cgcaaccgtg agccagcgcc
      541 cgcccgcgtc cacgtcgtac cagtcgccgc cgacgcggtt cgcctgccgt tggaggggga
      601 cgacgcgcac gccgtcgatc tccggcagct tccgctggag cagcttctgc tggatcgacg
      661 cggcgatctc gagctcgcgg tcctgcaggg cgagctgctg gagcttcgcg aggccggact
      721 gcatctcgat gaatcgttgc gcgagctcct cgacctcgtc gccggagcgg atgtcgagct
      781 cttggttgag gtcgccgcgc tcgatcgcgc gcgccgcgtc gacgagctgc ccgaggttga
      841 aggcgagacg gcgagggagc gcgatcgcga gcgcgacgcc cgcgagcagc gcgccgatcg
      901 agacggcgac gaggatcacg atcgtctggt tcgaacgctg gtcgacctcg ttctcggtca
      961 cggcgaccac gcggtcctgc tcgatcgcga gctgccggcc gagcgacccg atgctcgcga
     1021 tcgcgggcgt gccctcgtcg cgcaggatgc ggatcgcctg cgaccgcttc ccgtccgacg
     1081 cgagcgcgac cacgcggtcg acgacctcct ggaactcgtc gccggcctcg gtcaagtcct
     1141 cgaggagcac gcgctcggcc gggtcggtga agagccgttc ggccctctgc tcccaggtct
     1201 ccgcgttgcc gacttcgctc cggtactgct cgagcgcgcc ggtctcggcg gtcaggtaca
     1261 gcccgaccga cgccgactgc gcggtgtacg agcgcacgat ctcgtccacg gcgacgaggc
     1321 ccggcacggc gtcgttgaag accttccgtt gcaccgagcg cagcgacgtg aactgggcga
     1381 tcgtgaccca ggccaggatg ccgatcaggc ccaggaggat caggaacccg gccgacacct
     1441 tccaccggag ggacaacctc atcccgccag tatcctcgct gcgaccgcgc tatagccagg
     1501 agcccggacc gctcgagagg gggagatcgc cgtgccgccg tggggacgac tgctcgcgtc
     1561 cggcgcggcc ctgggagccg ccggcgccgc ctacgccctc gccgaggccc gtgcgttccg
     1621 cctgcaggag caccggctcc cggtccggcc cggggcgccg cgcacgaccg tgctgcacgt
     1681 ctccgacacg cacatgcagg agcgcgaccg cctgaaggcg cagtggctgt cgaggctccc
     1741 ggagctcctc gagatcacgc ccgacgtcgt cgtcgccacg ggcgacctca tcgaggaccc
     1801 gacggggatc gacccgctcg tcgaggcgct gtcgggcctg caggcccgct acggcaagtg
     1861 gtacgtgctc ggctcccacg actacttcca ctcgacgttc ccggggttct tcaagtactt
     1921 cagcggcaac cgcgaggtcg tgagagccaa gccggccgac acgccgcggc tcgaggccgg
     1981 gctgaaggag accggatggg tcgcgctgac gaacaggtcg gagaccgtgg acacaccggg
     2041 cggccggatc aggctcagcg gcgtcgacga cccctacatc cgccgcgacg acacgagaca
     2101 cctcggcagg gagcagggtg acgcgctcgc gatcggcgtc atgcacgctc ccgaggtcgt
     2161 ctcggagtac gcgctgcacg gatacgacct cgcgctcgcc ggccacacgc acgggggcca
     2221 ggtgcgcgtg ccgttcgccg gagcggtggt tacgaacagc gacctgccgt gcgcgctcgc
     2281 ggcggggccg acgagagtgg ggtcgtcgtg gctgcacgtg agccccggcg tcgcgcaggg
     2341 caagttcgtc ccgctgcgct tcaactgccc gccggaggtc acgctgctga cgctggagcc
     2401 gagctaggcg agccgatgca tctagtctcc ggctcgatgc gtgcccttcg tcttctcctc
     2461 gtcgccgtgc tgctcgcggc gttgccgccg ctcaccgcgc gcgcggcccc ccggcccccg
     2521 ccacagaacg cgtcggtccc gggccaggtc acggtcgtga ccgtcaacgc gaagcagaac
     2581 cgcatcctcg gcctcgcgcg cttcctcgcg ctgttcgagc tgccgaaggc gctgcgcttc
     2641 cgtcccgagg ctttcaacgg cggcttccgc ggcgcagtca ccgcacccga cgtgatcatc
     2701 gtccaggagg tgcggccgtc gaacctcgag atcctcgtcc ggctgctgcg ccagcgcttc
     2761 ccctacaggt acgaggcgct cgagcccgcc ggcgcggccg cggccttcgt ggtcaacacc
     2821 gacacggtca ctccgcaggg cgaggtgacg acgtgggacg acgtctgcac caccgaggag
     2881 acgccgaccg acaaccgcgc gacccgcgac tacccgatcg gccgcttcgt cgaggtcgcc
     2941 accaacgcgc cgttcgtcgt cgccggcatg cacatggcga agcgctacga cacgaccggc
     3001 cagccgaact gcgtcccccg caacatggag aggttcaagg aggaggtcgc cggcgagacc
     3061 gctccggtga tcttcggcgg cgacttcaac cggcgcgcgg tcctcgaccc ctacgagtgc
     3121 gaccccgagg agcggggcac gccgaacccc tggtacgaga tcctgaccgc gccgcaggac
     3181 gggagccgcg tgttcgtcga cgcggtcagg caatggcacc gcggacacgg gacgtcgatg
     3241 gcgaccgagt ggacacacga gcagcgccag gcgcgcgtcc tctgcgacga gaccacacgc
     3301 ttcaagcggt cgcgcatcga ctacctcttc gccgccgggg cggctgtcgc cgaagcgcac
     3361 tcggaccacc ccggctgggc gggcgctgaa gccggcgtca ggctcgggac cccctactcc
     3421 gaccaccgct acgtatgggg ccgcttcgtc gtgtcgggcc cgccccggcc gctgcaaccc
     3481 gcagtaaccc ccggagcaga cgggcgcatc gacgtgacct ggggagccgt cgaaggcgcg
     3541 gccggctacg tcgtctaccg cgctctgcgc ggtcacgcgt acacggtcct cgcgcaggtc
     3601 ggcgcggacg tgaccacgtt cggagacgtc ttcaccgagc acgagcagtc gtaccggtac
     3661 gcgatcgccg cggtcggcgc agacggcggg atcggccagg agtcgcggcc cgccttcggg
     3721 atcgcggaca agaaggggcc tcacgtcgtg cgcgtgaccc cgtcgcgcaa cgccaccgcg
     3781 atcgaccccg gcgtcacgat cctgatccgc ttcgacgagc gcgtcgcgtc cggcagcgtc
     3841 gccgccgaca ccgtgcgcct ctggtacgga cgccggcgca tcccgggcat cacgacgatg
     3901 gcgggggccc gcctgctgca gctcgacccg gcgggccggc tcgacaaggg caagacctac
     3961 cgcgtcgtcg tggacccggt gagggacgtg ctcggcaacg cggggccgcg cgaggcttgg
     4021 cgcttcacga cggtcgagcc gccgcccccg ccgccgaagc gacgccgcta gccccacccg
     4081 cgatgtggga tgcttcaaga cacgacgacc ggtcgcctcg gcgagcggcc agcatcaacg
     4141 ggaagtggcg cagtttggca gcgcgctgcg ttcgggacgc agaggtcggg ggtt
//
LOCUS       DASQDO010000106         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_684045, whole genome shotgun sequence.
ACCESSION   DASQDO010000106 DASQDO010000000
VERSION     DASQDO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684045"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VEV43_03300"
     CDS             <1..327
                     /locus_tag="VEV43_03300"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HYP22576.1"
                     /translation="RVAALIGVDVAVVVSDTFGRAWRLGQTDVAIGVAGIDPFVDYRG
                     TTDEQGRTMTATRICIADELAGAAEMVMGKAEGVCAAVVKGARVTRARGAATQIVRPP
                     SEDLFR"
     gene            324..929
                     /locus_tag="VEV43_03305"
     CDS             324..929
                     /locus_tag="VEV43_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYP22577.1"
                     /translation="MNLADAVAARRSIRAFEDRAVPRDSVLRAIALAVQAPAPHHSAP
                     WRFSLLDDDGAKDAFSRAMGAAWRADLTADGHPPEKVEAILTRSHRMLTGTPLLTVCS
                     ADMSRAHDYPDERRRLAEWSLFAHATGAALQVYMVALAEEGIASCWISAPVFCRDVVK
                     EHLGLPGSVEPQALVLVGYPSPEYSPRARGPVDPHNFVVGS"
     gene            945..1598
                     /locus_tag="VEV43_03310"
     CDS             945..1598
                     /locus_tag="VEV43_03310"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HYP22578.1"
                     /translation="MADETPTSKKLLEKQQRRAAEEERRREQQKAARKRNLVTLVIAL
                     AVLVLVATLVVQNRDEGGSVPSVGRGEAGCTEIEEHDIEGQEHVDVTPQYQTNPPTSG
                     NHLQPPASPGFFDEAVDPGALVHNMEHGQIIFWYDPEAPADVLDALEEVVDQEFEASI
                     AVPWDELESPHNFAMTAWGASQQCRDVSQSVVNAVREEYQGRGPENVGIKPFEAPEA"
BASE COUNT          270 a          610 c          613 g          235 t
ORIGIN      
        1 cgggtcgccg cgctgatcgg cgtcgacgtc gcggtggtcg tcagcgacac gttcggccgc
       61 gcctggaggc tcgggcagac cgacgtcgcg atcggtgtcg ccggcatcga cccgttcgtc
      121 gactaccgcg ggacgacgga cgagcagggg cggacgatga ccgcgacgcg catatgcatc
      181 gccgacgagc tggcgggcgc cgccgagatg gtgatgggaa aggcggaggg cgtgtgcgcg
      241 gccgtcgtga aaggagcgcg ggtcacccgc gcgcgcggag ccgcgacgca gatagtccgg
      301 cccccgtccg aggacctgtt ccggtgaacc tcgccgacgc cgtcgccgcg cggcgcagca
      361 tccgcgcgtt cgaggaccgt gccgtgccgc gggactccgt gctacgcgcg atcgcgctcg
      421 cggtccaggc ccccgcgccg caccactcgg ctccgtggag gttctcgctg ctcgacgacg
      481 acggtgccaa ggacgcgttc tcgcgcgcga tgggggccgc gtggcgcgcc gacctcaccg
      541 ccgacggcca ccctcccgag aaggtcgagg cgatcctcac gcgctcgcac cggatgctga
      601 ccgggacgcc gctcctgacc gtgtgctccg ccgacatgtc gcgggcgcac gactatccgg
      661 acgaacggcg ccggctcgcc gagtggagcc tgttcgccca cgcgacgggt gccgctctcc
      721 aggtgtacat ggtggctctc gccgaggagg gcatcgcctc ctgctggatc tcggcccccg
      781 tgttctgccg cgacgtcgtg aaggagcacc tcggcctgcc gggctccgtc gagccgcagg
      841 cgctcgtcct cgtcggctat ccgtcgccgg agtactcgcc gcgggcccgg ggcccggtgg
      901 acccccacaa ctttgtcgtc gggtcctgat agcctccctc ggcgatggct gacgagaccc
      961 ccaccagcaa gaagcttctc gaaaaacagc agcggagggc cgccgaggaa gagcggcgcc
     1021 gcgagcagca gaaggccgcg cgcaagcgca acctcgtgac gctcgtgatc gcgctcgcgg
     1081 tcctcgtgct cgtcgccacg ctcgtggtcc agaaccgcga cgagggcggg tcggtgccca
     1141 gcgtcggccg tggcgaggcc ggatgcaccg agatcgagga gcacgacatc gaggggcagg
     1201 agcacgtcga cgtcacgccg cagtaccaga ccaacccgcc gacctccggc aaccacctac
     1261 agccccccgc gtcgccgggc ttcttcgacg aggcggtcga cccgggtgcg ctcgtccaca
     1321 acatggagca cgggcagatc atcttctggt acgaccccga ggcgccggcc gacgtgctcg
     1381 acgcgctgga agaggtcgtc gaccaggagt tcgaggcttc gatcgcggtg ccgtgggacg
     1441 agctcgagtc gccgcacaac ttcgcgatga cggcgtgggg cgcctcgcag cagtgccgcg
     1501 acgtgtcgca gtccgtcgtg aacgccgtcc gcgaggagta ccagggcagg ggcccggaga
     1561 acgtcgggat caagccgttc gaagcgcccg aggcctagcg gccgccacgt agatcgcgac
     1621 gtttcagccg ggggtccgaa gaccccgcca catatactga atcgtcgctt cgaccaacgc
     1681 agcgggggta gcagttcatg acgatcacgc cggacgactt gataagca
//
LOCUS       DASQDO010000107         5181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_256026, whole genome shotgun sequence.
ACCESSION   DASQDO010000107 DASQDO010000000
VERSION     DASQDO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256026"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4367)
                     /locus_tag="VEV43_03315"
     CDS             complement(<1..4367)
                     /locus_tag="VEV43_03315"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYP22579.1"
                     /translation="MPVQPGMAQPEQGEGGVPESAGPPAKLPPGPTESARHSEEGRPP
                     EPQGEAHGGKPAAPLGAQSGPDASVRGLSEAAELRGPSARFFEDEGHEPHVVQLYTDP
                     INYRDENGEWQPIDSSVVATEGGYTNAANSFDLFFPGTLTPANPVVLDFPEGSLSFAL
                     IGGQPAEAVASTHSVTYEGAHPHVDVAFDVAADGVKERTILRTSEAPEVLRYLIQTKG
                     VTLSEGAAGSIAIARGGTTFGHIPPAFAFDSGTDGETREPATTFDVAMNLRNRGPGAY
                     TLELRVDSRWLNDPERVFPVTVDPSVSTRCNNYPEPCPEDHGAPLIKDTFVRDGLNTR
                     QDSAPYLHVGRTGGGDNRAFMTFDVRQEIRKIGDLIYDAKFDINVDDSWSAGAAPLEL
                     RRITQDWDAATTWEYARPDVHSKVWAQDDGCSAMGCKDGWTQWDVSDLMQYWIDTKVL
                     SNHGWELSVSSSSPHAWKTYVSGDYANADRRPHVNVWVNAMPGTKEFAMDDADWPKLE
                     LAWEAIPNSTVLEIDRPKLEIRDLIDKNGDRIFVRYQVSKNPAGSFGSGIEYDSGWIE
                     EKHSLRVPSGYLAEGTHYWRVMAGDACPEKYKDPDEPPLGNGLYICDDLGADNTRAVT
                     TNERPISEVRSFTIRDHGKGLGKDPRWAMWSKPLGNGMQLSVNQSNGNAVLEYAIDSL
                     GTSEAPVKVGLTHNTHQAMRVQASKEQAVAGLPPGWEIAAGPAADPQQMPVRIKRLSY
                     TTFTGTVTYGVAVVYESGKRDVLIDTGDGVFSMAGPLAPTVRSNGTAGWTMTTYSGGT
                     YTFSAAGALTKARPSSSRPGMPGFDYTYVDGRIRHLVDPVGRKLTFNYEIPAGGTKSL
                     LKEVVAFDDQTDPSSKLVWTIDYENERIVSIKDPENGTVKLAYDPSERLSKIYDAVQA
                     AATAPLPTAIEYDTTVFGTDRAAVTKVALPSVDVVPPEDQRSSSDPYATSPSTPRWEF
                     SYTKATSNANYSKETKTTGPRGVLTSDVEDHMVVTDFNDIGLPIMERGPRIGGEFGGW
                     PVTEKVWDFETGNLVCHRGPAANKIASVACGSSDGLQTQYEYQKRAPYLLTKVTSASD
                     EPNGARLVTTYGYDEGFSGLQAHYYKNNTLTGIPDAIGTVSTVSENWGEGGPSQIDND
                     SDFSVRFFGEWKPPNATQAKDYQFRVTGEDEARLIVGKEILVDCISVGVLRKCPTGTQ
                     NTVTAKTVGPAAVPIVVELRAATGPAHVKLEWKRPDGVFEIVPSSALSSATDARTSEK
                     VGTVAAPGAFSTRTYEYATGESKVRRLPTRVELTGGPETPRTVAQSASYTYDDHGRIK
                     TETDATGRDVVHSYDVHGPCEHTTTDRVDLVTTRVCNLAGDVLSETVHVGGVTVNGST
                     DVEQRTTTTTYTSLGRVDRIDFADGGYVDHDYDLAGRLVKKTVLQSAGGMMRETEYVR
                     NAQGWVTEERLPDPDGTGP"
     gene            complement(4667..>5181)
                     /locus_tag="VEV43_03320"
     CDS             complement(4667..>5181)
                     /locus_tag="VEV43_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP22580.1"
                     /translation="PAEDTVIEPQKFVDEIVTVGASCPAIDGFDPWANEGAGAASGAG
                     EMAVYSARETEIDVVAPVAGIWAPKYVTPAAGNQDTTEPRPGASDPEATANNRLWYGV
                     FGGTSAAAPCISGVVALMLEVDPTLTPAEIDSILKKTARDGGPAGWDSAWGWGEVDAF
                     QAAKRALHGR"
BASE COUNT          753 a         1766 c         1696 g          966 t
ORIGIN      
        1 atcggccccg tgccgtcggg atccgggagc cgctcttcgg tcacccaacc ctgagcgttc
       61 cttacgtact ccgtctcacg catcatcccg ccggcggact ggaggaccgt cttcttgact
      121 agtcgtccgg cgaggtcgta gtcgtggtcg acgtacccgc cgtcggcgaa gtcgatgcgg
      181 tccactcggc ccagcgaggt gtaggtcgtg gtggtcgttc gctgctcgac gtcagtcgag
      241 ccgttgaccg tcacccctcc gacgtggacg gtctcggaca agacgtcccc agcgaggttg
      301 cagaccctcg tcgtgacgag gtctacccgg tccgtggtgg tgtgctcgca gggaccgtga
      361 acgtcgtacg agtggacgac gtccctcccg gttgcatcgg tctcggtctt gatccggccg
      421 tggtcgtcgt aggtgtacga ggcgctctgg gcgacagtcc gcggagtctc gggtcccccc
      481 gtaagctcca cgcgagtggg gagcctgcgc accttcgact cgcccgtcgc gtactcgtac
      541 gtccgggtcg agaacgcgcc gggagccgcg acggttccga ccttctcgga ggtcctcgcg
      601 tcggtagccg agctcagggc agagctcggc acgatctcga acacgccgtc ggggcgcttc
      661 cactcgagct tgacgtgggc gggcccggtg gcggcgcgca gctccacgac gatcgggacc
      721 gcagccggtc ctacggtctt ggccgtgacg gtgttctggg tcccggtagg gcactttcgg
      781 aggactccta ctgagatgca gtcgaccagg atctccttgc cgacgatcaa gcgggcctcg
      841 tcctcgcccg taacccggaa ctggtagtcc ttcgcttgcg tcgcgttcgg cggcttccac
      901 tcaccgaaga accgaaccga gaagtcgctg tcgttgtcga tctgcgaagg tccgccctcg
      961 ccccagttct cggagaccgt cgagacggtg ccgattgcgt cggggatgcc cgtgagcgtg
     1021 ttgttcttgt agtagtgcgc ctgaaggcca ctgaaaccct cgtcgtaccc atacgtcgtc
     1081 acgagacgtg ctccgtttgg ctcgtccgag gctgaggtca ccttcgtcag gagatacggc
     1141 gcccgcttct ggtactcgta ctgtgtctgc agcccgtcgg acgacccgca cgcaaccgaa
     1201 gcgatcttgt tcgccgctgg tccccggtgg cagaccaggt ttccggtctc gaagtcccac
     1261 accttctccg tgacgggcca tccaccgaac tcgcccccga tgcgcgggcc gcgctccatg
     1321 atcgggagac cgatgtcgtt gaagtccgtg acgaccatgt ggtcctcgac atcgctcgtc
     1381 aggacgccgc gcgggccggt cgtcttcgtc tccttcgagt aattggcgtt cgaagtcgcc
     1441 ttcgtgtagc tgaactccca cctcggcgtg ctcggcgacg tggcataggg gtcgctcgag
     1501 ctgcgctggt cctccggtgg gacgacgtcc acgctcggga gagcgacctt cgtcaccgcg
     1561 gcacggtcgg tcccgaacac agtggtgtcg tactcgatgg ccgtgggaag cggcgcggtc
     1621 gcggcggcct ggacggcgtc gtagatcttc gagaggcgct cgctcgggtc gtacgcgagc
     1681 ttcacggtcc cgttctcggg atctttgatc gagacgatcc tctcgttctc gtaatcgatg
     1741 gtccatacga gcttgctcga cgggtctgtc tggtcgtcga aagcgacgac ctctttcaga
     1801 agcgacttcg tgcccccggc ggggatctcg tagttgaacg tcaacttgcg acccaccgga
     1861 tctaccaagt gacggatgcg cccgtcgacg tacgtgtagt cgaagcccgg catccccggc
     1921 ctcgacgacg agggccgcgc cttggtcaag gcgccggccg ccgagaacgt gtaggtgccc
     1981 cccgagtacg tcgtcatcgt ccatccggct gtcccgttcg agcgcaccgt gggtgccaag
     2041 ggacctgcca tcgagaacac gccgtcgccc gtgtcgatca acacgtcgcg ctttccggac
     2101 tcgtagacga ccgcgacgcc gtacgtgacg gtcccggtga acgtcgtgta agagaggcgt
     2161 ttgatccgca cgggcatctg ctgcgggtcg gcagcgggcc cggccgcaat ctcccatccc
     2221 ggtgggagcc cggccacggc ctgctccttg ctcgcctgga ctcgcatcgc ctgatgcgta
     2281 ttgtgggtga ggccgacctt gaccggggct tccgaggttc cgagggagtc gatcgcgtac
     2341 tccagcacgg cgttcccgtt ggactggttg accgacagct gcattccgtt gccgagcggc
     2401 ttgctccaca tcgcccagcg cgggtccttg cccagcccct ttccgtggtc cctgatcgtg
     2461 aaggagcgga cctcggagat cggacgctcg ttcgtcgtga cggcacgcgt gttgtcggcg
     2521 ccgaggtcgt cgcagatgta cagaccgttg ccgaggggcg gttcgtcggg gtccttgtac
     2581 ttctccggac aggcgtcgcc cgccatgacc cgccagtagt gagtgccctc cgcgaggtag
     2641 cccgacggga cgcgcagcga atgcttctcc tcgatccagc cgctgtcgta ctcgatcccg
     2701 ctcccgaacg accccgcggg gttcttcgac acctggtacc ggacgaagat cctgtcgccg
     2761 ttcttgtcaa tcaggtctct gatctcgagc ttgggcctgt cgatctcgag gacggtcgag
     2821 tttggaatcg cttcccacgc cagctcgagc ttcggccagt ccgcgtcgtc catggcgaat
     2881 tccttcgtcc cgggcatggc attcacccag acgttcacgt gcggccgccg gtccgcgttc
     2941 gcatagtcgc cggacacgta ggtcttccac gcgtgcggcg aggacgagct cacggatagc
     3001 tcccacccgt ggttgctcaa gaccttcgtg tcaatccagt actgcatgag gtcggagaca
     3061 tcccactggg tccagccgtc cttgcaaccc atggccgagc agccgtcgtc ctgtgcccag
     3121 accttcgagt gcacgtcagg acgcgcgtac tcccacgtcg tggctgcgtc ccaatcttgg
     3181 gtgatcctac ggagctccaa cggcgctgcg ccggccgacc agctgtcgtc gacattgatg
     3241 tcgaacttgg catcgtagat gaggtcgccg atctttcgga tctcctgtcg cacgtcgaac
     3301 gtcatgaacg cgcggttgtc cccgccgccg gtcctgccca cgtggaggta cggcgcagag
     3361 tcctgccggg tgttgaggcc gtcccggacg aacgtgtcct tgatcaacgg agccccgtga
     3421 tcctccggac aaggttcggg gtagttgttg caccgggtgc tcacggacgg gtcgaccgtc
     3481 actgggaaga cgcgctcggg gtcgttcaac caacgggaat ccacccgaag ctcgagggtg
     3541 tacgcgccgg gcccccggtt cctgaggttc atcgcgacgt cgaaggtcgt cgcgggctca
     3601 cgggtttccc cgtcggtgcc ggagtcgaac gcgaaggccg gcgggatgtg gccgaacgtg
     3661 gtgcccccgc gtgcgatcgc gatggagccg gccgccccct ccgagagggt cacgcctttc
     3721 gtctggatca agtatcggag cacctccggc gcctcgctcg tcctgaggat ggtgcgttcc
     3781 ttgaccccgt cggctgcaac gtcgaaggcc acgtccacgt gagggtgcgc gccctcgtag
     3841 gtcaccgagt gcgtggacgc aacggcttcg gcaggctgtc ccccgatcag cgcgaacgag
     3901 agcgacccct cggggaagtc cagcacgacg ggattcgcag gagtgagcgt cccggggaag
     3961 aagaggtcga acgagttcgc cgcgttcgtg tagccgcctt cggtggcgac cacgctgctg
     4021 tcgatgggtt gccactcgcc gttctcgtcc ctgtagttga tcgggtcggt gtagagctgg
     4081 acgacgtgcg gctcgtgccc ttcgtcctcg aagaagcggg cactcgggcc gcgcagctcg
     4141 gccgcttcgg agagcccacg cacggacgcg tccgggccgc tctgggcccc caacggtgcg
     4201 gcaggcttcc ctccgtgcgc ctcgccctgg ggctccggcg ggcgaccctc ttccgaatga
     4261 cgcgccgact ccgtcggccc ggggggcagc ttcgcgggcg gccccgcgct ctcgggaacg
     4321 ccgccctcgc cctgctccgg ctgcgccatc ccgggttgga ccggcacgag cagcattgca
     4381 actgcggtcg tcacggccag aaatgcccgc gcgcggactg acctgttctc cttggacacc
     4441 aagaaccccc actcggatat tcgtcgctag aggtccctta tatgtgagcc gcggcccggg
     4501 cgacagacga aaagagcgaa gtagtcggca aaatgcctcg aagggactac gagcccgaca
     4561 agcggtaggt gggcaagcaa acgatcgctc cgcacgccgc cgcgaagtgc gcgcgaagcg
     4621 tcgcgtatac cggccgcgca gggcccgttc gctgcccgga acgcgtctac cggccgtgca
     4681 gggccctctt cgccgcctgg aacgcgtcta cctcgcccca gccccaggcg ctgtcccagc
     4741 ccgccgggcc cccgtcgcgc gccgtcttct tcaggatcga gtcgatctcg gccggcgtca
     4801 gcgtcgggtc gacctccagc atcagcgcga ccactcccga gatgcagggc gctgccgccg
     4861 aggtgccgcc gaacaccccg taccagaggc ggttgttcgc ggtcgcctcg gggtcggacg
     4921 cgcccgggcg aggttcggtc gtgtcctggt tacccgccgc cggcgtgacg tacttcggcg
     4981 cccagatgcc tgcgaccggc gcgaccacgt cgatctccgt ctcccgtgcc gagtagacgg
     5041 ccatctcgcc ggcgccggag gccgcgcccg cgccctcgtt cgcccatggg tcgaacccgt
     5101 cgatcgcggg acaggacgcg ccgaccgtga cgatctcgtc gacgaatttc tgcggctcga
     5161 tcacggtgtc ctccgcgggg c
//
LOCUS       DASQDO010000108         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_500688, whole genome shotgun sequence.
ACCESSION   DASQDO010000108 DASQDO010000000
VERSION     DASQDO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500688"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1412)
                     /gene="ahcY"
                     /locus_tag="VEV43_03325"
     CDS             complement(156..1412)
                     /gene="ahcY"
                     /locus_tag="VEV43_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYP22581.1"
                     /translation="MKSLLKDVALADEGRSKVAYAGRRMPVLTAIREEFAASQPFKGR
                     RISACLHVTTETANVVRALIEGGAEMAVCASNPLSTKDDVAAAIHVDFGAEVYAINGC
                     DRDTFYKQIEGVLAFEPDIIIDDGADLTSTIHRERPELLEKIVAGTEVTSTGVLRMRN
                     MHRDGRLKYPVVAINDAKTKHLFDNRYGTGQNTIDGIMRGAHTLLAGSVLVVSGYGWC
                     GRGIAMRADGMGARVIVVEIDPIKALEAVMDGYQVMPLADAAPRGDLFVTATGNRDVI
                     RYEHMEKMKPGAIICNSGHFDVEIDMETLEKNAVSKESIREHAVQYELPTGTSVVVLA
                     EGRLVGQAAAEAHPAEVMDMTFSSQALAMAWLAENADTLPLGVHTVPADIDDRVSRLK
                     LESFGVTLDELTDAQQEYLDAWEVGT"
     gene            1490..3181
                     /gene="metG"
                     /locus_tag="VEV43_03330"
     CDS             1490..3181
                     /gene="metG"
                     /locus_tag="VEV43_03330"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYP22582.1"
                     /translation="MARHLITSALPYINGVKHLGNLVGSMLPADVYARFLRLTGEEVL
                     FICATDEHGTPAELAAIEQGLPVEEYCRQQHAIQKDVYERFLLSWDHFGRSSSQQNHE
                     LTQHFAAELERHGFIEERPMKQVYSVDDGRFLPDRYVIGTCPHCGYEAARGDQCENCT
                     RLLDPTDLIDPRSAVSGSTRIEIRETRHLFLRQAVLADEIRAWIDRHEDWPPLVKSIA
                     YKWLDEGLRDRCITRDLEWGVPVHREGFEDKVFYVWFDAPIEYLGATKEWADLDPSAR
                     DWKSWWWEADDVTYSQFMGKDNVPFHTVSFPATIIGSREPWKLVTYVKAFNWLTYYGG
                     KFSTSRGVGIFTDQAIDLLPADYWRYFLMANAPETSDSSFTWEGFAGTVNKDLADTLG
                     NFVNRCLTFTNKHFGDALPDGGEAGDAEAELARTLSEKVGAYTALMHGKEFRKATAEL
                     RAIWSTGNAYWEQSEPWKVVKADRDAGAVIMRTAANLVRLFALLSAPIVPESAARILD
                     SVSPGEEPRWPSDDVAAELTRLAPGAPYSVPPVAFKKIEDTDVKSWEERFGAAGA"
     gene            complement(3178..>3589)
                     /locus_tag="VEV43_03335"
     CDS             complement(3178..>3589)
                     /locus_tag="VEV43_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22583.1"
                     /translation="YFSTHLCFSDYELFFPAIEGMHGCRQYAVGFANYDTTDLGLSDD
                     ARVGYSIIKRFRDLSYEPALGVGVIDIHTDFVEPPELVRDRILYAAEVFGDPARVHVT
                     PDCGLRTRSWEVAYRKCESMVAGTEMAKKELGSS"
BASE COUNT          559 a         1250 c         1195 g          585 t
ORIGIN      
        1 aacgatatgg gacagatgcc gacgctcgac ggccgccgag ggctcggcgc atgggtggcg
       61 acgccgtacc ctctgcaaga ttttcggttc gccgggttgt tcgtaaagat tcgctagata
      121 gttcgacgac gaccgaccga agcggccctc gcgggctagg tcccgacctc ccacgcgtcc
      181 aggtactcct gctgggcgtc ggtcagctcg tccagcgtca cgccgaacga ctcgagcttg
      241 agccgtgaca cccggtcgtc gatgtcggcc gggaccgtgt gtacccccag gggcagcgtg
      301 tccgcattct cggccagcca cgccatcgcc agcgcctggc tcgagaacgt catgtccatc
      361 acctcggcgg ggtgcgcttc cgcggcggcc tgtccgacga gccggccctc ggcgagcacg
      421 acgaccgacg tcccggtcgg cagctcgtac tggaccgcgt gctcgcggat cgactccttc
      481 gacacggcgt tcttctcgag cgtctccatg tcgatctcga cgtcgaagtg gcccgagttg
      541 cagatgatcg cgcccggctt catcttctcc atgtgctcgt agcggatgac gtcgcggttc
      601 cccgtcgccg tcacgaagag gtcgccgcgc ggcgcggcgt ctgcgagcgg catgacctgg
      661 tagccgtcca tcaccgcctc gagcgccttg atcgggtcga tctcgacgac gatcacgcgg
      721 gcccccatgc cgtcggcgcg catcgcgatc ccgcggccgc accagccgta gccggagacg
      781 acgagcaccg atcccgccaa cagcgtgtgc gcgccgcgca tgatcccgtc gatcgtgttc
      841 tggccggtcc cgtagcggtt gtcgaagagg tgcttcgtct tcgcgtcgtt gatcgccacc
      901 acggggtact tcagccgacc gtcccggtgc atgttgcgca tgcgcagcac accggtgctc
      961 gtcacctcgg tgccggcgac gatcttctcg agcagctccg gccgctctcg gtggatcgtg
     1021 ctcgtgaggt cggcaccgtc gtcgatgatg atgtccggct cgaacgcgag cacgccttcg
     1081 atctgcttgt agaaggtgtc gcgatcgcac ccgttgatcg cgtagacctc ggcgccgaag
     1141 tcgacgtgga tcgccgcggc gacgtcgtcc ttggtggaca aggggttcga ggcgcacacg
     1201 gccatctccg cgccgccctc gatcagggcc ctgaccacgt tcgcggtctc cgtcgtcacg
     1261 tggaggcagg ccgagatccg ccggcccttg aacggttggc tggcggcgaa ctcctcgcgg
     1321 atcgccgtga gcaccggcat ccgccggccg gcgtaggcga ccttcgaccg gccctcgtcc
     1381 gcgagcgcca cgtccttgag aagcgacttc atgtatgggt cctctctcat tcgttgtcgg
     1441 tgcgcggatg ctacctccgc ggcgcctgcg cccttgtagg gtcgcccccg tggcacgaca
     1501 cctcataacg agcgcgctcc cctacatcaa cggcgtcaag cacctcggca acctcgtcgg
     1561 gtcgatgctg cccgccgacg tctacgcgcg cttcctccgg ctcaccggcg aggaggtcct
     1621 gttcatctgc gccacggacg agcacgggac gcccgcagag ctcgccgcga tcgagcaggg
     1681 gctccccgtc gaggaatact gccggcagca gcacgcgatc cagaaggacg tctacgagcg
     1741 cttcctgctg tcgtgggacc acttcggccg gagctcgtcg cagcagaacc acgagctgac
     1801 gcagcacttc gccgccgagc tcgagcgcca cggcttcatc gaggagcgcc cgatgaagca
     1861 ggtctactcg gtcgacgacg gccggttcct gccggaccgc tacgtgatcg ggacctgccc
     1921 ccactgcggc tacgaggcgg cccgcggcga ccagtgcgag aactgcacgc ggctgctcga
     1981 cccgacggat ctgatcgacc cgcgctcggc ggtcagcggg agcacgcgca tcgagatccg
     2041 cgagacccgg cacctcttcc tgcgccaggc cgtcctcgcc gacgagatcc gagcgtggat
     2101 cgaccggcac gaggactggc ccccgctggt caagtcgatc gcctacaagt ggctggacga
     2161 gggcctgcgc gaccggtgca tcacccggga cctcgaatgg ggcgtccccg tccaccgaga
     2221 gggcttcgag gacaaggtct tctacgtgtg gttcgacgcg ccgatcgagt acctcggcgc
     2281 cacgaaggag tgggccgacc tcgacccgtc tgcgcgggac tggaagtcgt ggtggtggga
     2341 ggcggacgac gtcacctact cgcagttcat gggcaaggac aacgtcccat tccacaccgt
     2401 gtcgttcccg gcgacgatca tcgggtcgcg cgagccgtgg aagctcgtca cctacgtcaa
     2461 ggccttcaac tggctcacgt actacggcgg gaagttctcg acgagccggg gcgtcggcat
     2521 cttcaccgac caggccatcg acctgttgcc cgcggactac tggcgttact tcctgatggc
     2581 gaacgcgccg gagacgtcgg actcgagctt cacgtgggag ggcttcgccg gcaccgtcaa
     2641 caaggacctc gccgacacgc tcggcaactt cgtcaaccgc tgcctgacct tcacgaacaa
     2701 gcacttcggc gacgcgttgc ccgacggcgg cgaggccggg gacgccgagg ccgagctggc
     2761 gcggacgctg tcggagaagg tcggcgccta caccgcgctg atgcacggca aggagttccg
     2821 caaggcgacg gcggagctgc gcgcgatctg gtcgaccggc aacgcgtact gggagcagtc
     2881 ggagccgtgg aaggtcgtca aggccgaccg cgacgcgggc gcggtgatca tgcggaccgc
     2941 cgcgaacctc gtgcggctgt tcgcgctgct gagcgcgccg atcgtccccg agtccgccgc
     3001 gcggatcctc gacagcgtct ctccgggcga ggagccgcgc tggccctctg acgacgtcgc
     3061 agccgagctg acccggctcg cccccggcgc gccctactcg gtcccgccgg tcgcgttcaa
     3121 gaagatcgag gacaccgacg tgaagtcgtg ggaggagcgg ttcggggcgg cgggggccta
     3181 gctagacccg agctccttct tcgccatctc ggtccccgcg accatgctct cgcacttgcg
     3241 gtacgcgacc tcccacgacc gcgtcctgag cccgcagtcc ggcgtgacgt gcacgcgggc
     3301 cgggtcgccg aagacctcgg ccgcgtagag gatgcggtcg cgcacgagct cgggcggctc
     3361 gacgaagtcc gtgtggatgt cgatcacgcc gacgccgagc gcgggctcgt acgacaggtc
     3421 cctgaacctc ttgatgatcg agtacccgac ccgggcgtcg tcggagaggc cgaggtcggt
     3481 cgtgtcgtag ttcgcgaacc cgaccgcgta ctgacggcaa ccgtgcatgc cctcgatggc
     3541 cgggaagaac agctcgtagt ccgagaagca caggtgcgtc gagaagtag
//
LOCUS       DASQDO010000109         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_807001, whole genome shotgun sequence.
ACCESSION   DASQDO010000109 DASQDO010000000
VERSION     DASQDO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807001"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VEV43_03340"
     CDS             complement(<1..439)
                     /locus_tag="VEV43_03340"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HYP22584.1"
                     /translation="MRWQRNVVAVVVAGLALGACGGSDDDAGIDVEQIAAPAAEPAFE
                     TSPSVAKTGRVEMEVARSEVAEAAQAVVDLATSPKVGGFLTSSVVDLEDEYGAAAILV
                     HVPAERFEQAVTGLGASGEVTRQEMAGESLPDPASTASSAERKE"
     gene            657..2255
                     /gene="pruA"
                     /locus_tag="VEV43_03345"
     CDS             657..2255
                     /gene="pruA"
                     /locus_tag="VEV43_03345"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HYP22585.1"
                     /translation="MKDYAPGSSERRSLTERLATMADERVDIPMVIDGRPVTSGDIYE
                     VVMPHDTKHVHGDAHQATAEHFAQAADAATRAHADWAALPWEDRAAIFLRAADLLAGP
                     WRDTINASTMLGQSKTVHQAEIDAACELIDFWRFNVEFAARIYAEQPLSPPGMWNQSD
                     YRPLEGFVFAVTPFNFTSIAANLPTAPALMGNTVVWKPSLTQAHSAHYTMKLLEAAGL
                     PPGVVNVVHGPGPEIGEAALSHEELAGIHFTGSTPTFNWMWRKVGENVERYRNYPRIV
                     GETGGKDFIVAHPSASVDALATAIVRGGFEYQGQKCSAVSRVYVASNLWEGLRERLAD
                     EVPAIPMGDVTDFGNFMGAVIDDRAFQKHDGALQEARSSSDLEVVAGGDADASTGYFV
                     RPTVVRTTDPGTRLLREELFGPIVTVYVYPEGEWDQTLDLVDSATPYALTGSVFAREE
                     SAIVEAKQRLRFAAGNFYVNDKPTGAVVGQQPFGGARRSGTNDKAGSMWNLIRWVSPR
                     SIKETFVPATDYRYPFMDSERTGP"
     gene            complement(2252..2641)
                     /locus_tag="VEV43_03350"
     CDS             complement(2252..2641)
                     /locus_tag="VEV43_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22586.1"
                     /translation="MPPLDLRPGELADEIVSLRHWEEGDVPALVRALQDGDVARWVLQ
                     IPWPYTDHHARSFVRMAARDRATGKAAHLAVVAGAGVAGSVALTSIDWDNRSAELGYW
                     TATERGHLRTSTGRRDSLLFGLLADDG"
     gene            2699..3328
                     /locus_tag="VEV43_03355"
     CDS             2699..3328
                     /locus_tag="VEV43_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP22587.1"
                     /translation="MTPDLGFVHRFEPGTTGATLLLLHGTGADENDLIPLGRAVAPGA
                     ALLSPRGQVLENGMPRFFRRLAMGVFDLEDLKTRTEDLAGFVERAAAHYEIDLTRTIL
                     LGYSNGANIGASLLLSRPGLVPAAALLHAMVPFRPDDMPAVAGTRVLITGGEQDPMIP
                     LEQTHELAEILKAGGAEVELSLEPGGHQLTNAEVEKVAGWVRRIAADAA"
BASE COUNT          510 a         1215 c         1174 g          462 t
ORIGIN      
        1 cctccttgcg ctcggcggag ctcgccgtcg acgccgggtc cggcaggctc tcgccggcca
       61 tctcctggcg ggtgacctcg ccgctggcgc ccagccccgt cacggcctgc tcgaatcgct
      121 ccgccgggac gtggacgagg atcgcggcgg cgccgtactc gtcctcgaga tcgacgaccg
      181 acgacgtcag gaagccgccg accttgggcg aggtcgcgag gtcgaccacg gcctgggcgg
      241 cctcggcgac ctcggagcgc gcgacctcca tctcgacccg gccggtcttc gccaccgagg
      301 gcgaggtctc gaacgccggc tcggccgcgg gggccgctat ctgctcgacg tcgatccccg
      361 cgtcgtcgtc gctcccgccg catgccccca gagcgaggcc cgccaccacc actgccacca
      421 cgttgcgctg ccaccgcatc gcgcaccccc ttcgctcgcg gccgcctgcg tcgctggagg
      481 cgccctacta catcagtagt agctactaca acgatagtag gacagagccg ccccccgtgt
      541 ccaggggcta cgctcgaagc tcttcagacc gcaagtggga cccccagaca agcgaggtgg
      601 ttcgcatcag ccccacgttc ggcatccccc ggcccccgtc cccgcgcaac gaacccgtga
      661 aggactacgc cccgggttcg tccgagcgga ggagcctgac cgagcggctc gccaccatgg
      721 ccgacgagcg cgtcgacatc ccgatggtca tcgacgggcg tcccgtcacg agcggcgaca
      781 tctacgaggt cgtgatgccg cacgacacca agcacgtcca cggcgacgcg caccaggcga
      841 cggcggagca cttcgcgcag gccgccgacg cagccacgcg cgctcacgcc gactgggccg
      901 cgttgccgtg ggaggaccgc gctgcgatct tcctgcgcgc cgccgacctc ctcgccggcc
      961 cgtggcgcga cacgatcaac gcgtcgacga tgctcggcca gtcgaagacc gtccaccagg
     1021 ccgagatcga cgcggcgtgc gaactcatcg acttctggcg cttcaacgtc gagttcgcgg
     1081 cgcgcatcta cgccgagcag ccgctgtcgc cgccggggat gtggaaccag tccgactacc
     1141 ggccgctcga gggcttcgtg ttcgcggtga cgccgttcaa cttcacgtcg atcgcggcga
     1201 acctgccgac cgcccccgcg ctcatgggca acaccgtcgt gtggaagccg tctctcacgc
     1261 aggcgcactc cgctcactac acgatgaagc tcctcgaagc cgcggggctc ccgccggggg
     1321 tcgtcaacgt ggtccacggc cccggccccg agatcggcga ggccgcgctg tcgcacgagg
     1381 agctcgccgg catccacttc accggctcga ccccgacgtt caactggatg tggaggaagg
     1441 tcggcgagaa cgtcgagcgc taccggaact acccgcgcat cgtcggtgag accggcggca
     1501 aggacttcat cgtcgcccac ccgtcggcgt cggtcgacgc gctcgcgacg gcgatcgtgc
     1561 gcggcgggtt cgagtaccag gggcagaagt gctcggcggt ctcgcgcgtg tacgtcgctt
     1621 cgaacctgtg ggaggggctg cgcgagcggc tggccgacga ggtcccggcg atcccgatgg
     1681 gcgacgtcac ggacttcggc aacttcatgg gggccgtgat cgacgaccgc gccttccaga
     1741 agcacgacgg cgcgctccag gaggctcgct ccagcagcga cctcgaggtc gtcgcaggcg
     1801 gcgacgccga cgcgtccacc ggatacttcg tgcgaccgac ggtggtgcgg acgaccgatc
     1861 ccgggacgcg gctgttgcgc gaggagctct tcgggccgat cgtcaccgtc tacgtctacc
     1921 cggagggcga gtgggaccag acgctggacc tcgtcgactc cgcgactccc tacgcactga
     1981 ccgggtcggt gttcgcgcgc gaggagagcg cgatcgtgga ggcgaagcag aggctgcggt
     2041 tcgcggccgg caacttctac gtcaacgaca agccgacggg cgcggtcgtg ggccagcagc
     2101 cgttcggcgg cgcccggcgt tcggggacga acgacaaggc cggctcgatg tggaacctga
     2161 tccggtgggt gagcccgcgc tcgatcaagg agacgttcgt cccggcgacc gactaccggt
     2221 acccgttcat ggactcggag cggaccgggc cctagccgtc gtcggcgagg aggccgaaca
     2281 gcagcgagtc ccggcggccg gtgctcgtac ggaggtggcc gcgctccgtt gcggtccagt
     2341 agccgagctc cgcggagcgg ttgtcccagt cgatcgaggt gagcgcgacc gatcctgcga
     2401 cgccggcgcc cgcgacgacg gcgagatgcg cggccttgcc ggtggcgcga tcccgggcgg
     2461 ccatgcggac gaaggaccgg gcgtggtggt cggtgtacgg ccacgggatc tggaggaccc
     2521 agcgtgcgac gtcgccgtcc tgcagggccc ggacgagtgc ggggacgtcg ccctcctccc
     2581 agtgccggag ggacacgatc tcgtccgcga gctcgccggg tctcaggtca agagggggca
     2641 ccttcccagt atcgaggccg cgggcagagc cacagggcgg atgcgcgagc atggacggat
     2701 gacccccgac ctcggcttcg tccatcgctt cgagcccggc accaccggcg cgactttgct
     2761 cctcctgcac ggcaccgggg ccgacgagaa cgacctgatc ccgctcggac gggcggtcgc
     2821 gccgggggcc gcgctgctca gcccccgcgg ccaggtcctg gagaacggga tgccccggtt
     2881 cttccgccga ctcgctatgg gcgtgttcga cctcgaggac ctgaagacgc ggaccgagga
     2941 cctcgcgggc ttcgtcgagc gggccgccgc gcactacgag atcgacctca cgcggacgat
     3001 cctcctcggc tactcgaacg gggcgaacat cggcgcgagc ctgctgctgt cgcggcccgg
     3061 cctcgtgccc gccgcggcgc ttctccacgc gatggtcccg ttccgcccgg acgacatgcc
     3121 cgccgtggcg ggcacccggg tgctgatcac cggcggcgag caggacccga tgatcccgct
     3181 cgaacagacg cacgagctcg cagagatcct gaaggcaggc ggtgccgaag tggagctgtc
     3241 gctcgagccc ggcgggcatc agctcacgaa cgcagaggta gagaaagtcg ccgggtgggt
     3301 gcgccggatc gccgccgatg cagcttaggg agatcgaccc ggcccggctc gagggccgcg
     3361 a
//
LOCUS       DASQDO010000110         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_990547, whole genome shotgun sequence.
ACCESSION   DASQDO010000110 DASQDO010000000
VERSION     DASQDO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990547"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VEV43_03360"
     CDS             <1..1031
                     /locus_tag="VEV43_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP22588.1"
                     /translation="AQTLPTGVNRIDGELSPTARINGIDERIDVDIAILDSGIDLDHP
                     DLNVVANTGCAQGGPSNRTCTTGAGDDNNGHGTHVAGIAAAIDNTIGVVGVAPGARLH
                     AVKVLDSRGSGYLSWIIAGIDWVTARSATIEVANMSLGGQGWSSAYRTAIANSVSRGV
                     VYYVAAGNEKRDIYGTDNTFGTSDDTIPASFPEVGTISALADFDGRPGGLTAASYSYG
                     APASCTENRDDSFACFSNFAKNNANSPVTSSGGKIDMMLPGTAIVSTYPGGYASTHGT
                     SQASPHAAGLAALYISRNGRATTASGVYSIRQALINMGYGQTSTNGLTTHDDPDLRKE
                     NIGWAATL"
     gene            complement(1032..1643)
                     /locus_tag="VEV43_03365"
     CDS             complement(1032..1643)
                     /locus_tag="VEV43_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22589.1"
                     /translation="MRARVLARVALAFVVAATAVPFVASADHTGVTDRNDTKGPFDVR
                     RVKVAGTRTPRYDVVTFPRWTTAQTWDRSYGLVYFDTFGGAAPDYYVMVRSDGYRMRA
                     RLWRDKARKKDRPLAFVKAWRPGKRSFAIRAPLRKMRFPEARLVYHWWVLTLHTGTSC
                     KRVCFDRAPDSGVVTEPAPGAGQQSPTPTPSVSVPTPSPSVSF"
     gene            complement(1640..2146)
                     /locus_tag="VEV43_03370"
     CDS             complement(1640..2146)
                     /locus_tag="VEV43_03370"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HYP22590.1"
                     /translation="MLVVPTLLAAAFVWAAVAKLGRFSAWRGALAGYRLPDGIAGPAG
                     ILVPAAELAAAFLLALGGDATRAGAALTVALLAAFSLAVLRARRLRGDRLPCGCFGGS
                     GSRDYRLMLVRNAFLGAVAGAVLLVPAVARYELEAPASSQVVPAVLAAAGVALIGWLA
                     VSVGRTGR"
BASE COUNT          390 a          909 c          834 g          296 t
ORIGIN      
        1 cggcgcagac cctgcccacg ggcgtcaacc ggatcgacgg cgagctcagc ccgacggccc
       61 ggatcaacgg gatcgacgag cgcatcgacg tcgacatcgc gatcctcgac agcgggatcg
      121 acctcgacca cccggacctc aacgtcgtcg cgaacaccgg gtgcgcgcag ggtgggccga
      181 gcaaccgcac gtgcacgacc ggcgcgggtg acgacaacaa cggccacggc acccacgtcg
      241 ccggtatcgc cgcagcgatc gacaacacga tcggcgtcgt cggcgtcgca ccgggcgctc
      301 gcctgcacgc cgtcaaggtc ctcgactcgc gcggcagcgg ttacctctcg tggatcatcg
      361 ccggcatcga ctgggtcacc gcccgctcgg cgacgatcga ggtcgcgaac atgagcctcg
      421 gcggccaggg ctggtcgtcg gcgtaccgga ccgcgatcgc gaactccgtc tcgcgcggcg
      481 tcgtctacta cgtcgccgcc ggcaacgaga agcgcgacat ctacgggacc gacaacacgt
      541 tcggcacctc ggacgacacg atccccgcgt ccttccccga ggtcgggacg atctctgccc
      601 tcgcggactt cgacgggcgc ccgggcggcc tcaccgccgc cagctacagc tacggcgctc
      661 ccgccagctg caccgagaac cgcgacgact cgttcgcctg cttctcgaac ttcgccaaga
      721 acaacgcgaa cagccccgtg acgtcgagcg gcggcaagat cgacatgatg ctgccgggca
      781 ccgcgatcgt ctcgacgtac ccgggcggct acgcgtcgac gcacggcacg agccaggcgt
      841 cgccgcacgc ggccggtctc gccgccctgt acatctcgcg caacggccgg gcgacgacgg
      901 cctcgggcgt gtactcgatc cgccaggcgc tcatcaacat gggctacggc cagacgagca
      961 cgaacggcct cacgacccac gacgaccccg acctccgcaa ggagaacatc ggctgggccg
     1021 cgacgctcta gctagaagct gacgctcgga ctgggcgtcg gaacactgac cgaaggcgtg
     1081 ggggtcgggc tctgttgccc ggcccccggc gccggttcgg tcaccacacc ggagtcggga
     1141 gcacggtcga agcagaccct cttgcaactc gtcccggtgt gcagcgtgag cacccaccag
     1201 tggtaaacga gccgggcctc ggggaacctc atcttccgta gcggggcgcg gatggcgaag
     1261 ctccgcttgc ccggccgcca cgccttcacg aacgcgagcg gccggtcctt ctttctcgcc
     1321 ttgtcacgcc agaggcgggc ccgcatccgg tagccgtcgg accgcaccat cacgtagtag
     1381 tcgggggccg cgccgccgaa cgtgtcgaag tacacgaggc cgtagctccg atcccacgtc
     1441 tgagcggtcg tccagcgcgg gaacgtgacg acgtcgtagc ggggggtccg ggtcccggcg
     1501 accttcaccc tccgtacgtc gaacgggccc ttcgtgtcgt tgcggtcggt cacgcccgtg
     1561 tggtccgccg acgcgacgaa cggaacggcc gtggccgcga cgacgaacgc gagggcgacg
     1621 cgagccagga cgcgcgccct caccggccgg tccttcctac ggacaccgcg agccagccga
     1681 tcagggcgac gccggccgcg gcgaggacgg cagggaccac ctgcgacgac gccggggcct
     1741 cgagctcgta gcgcgccacc gccggcacga gcaagacggc gccggccact gcgccgagga
     1801 acgcgttgcg gacgagcatc aggcggtagt cgcggctccc gctccctccg aagcatccgc
     1861 acgggagccg gtcaccgcgc agccgcctcg cccgcaggac ggcgagcgag aacgcggcca
     1921 gcagcgcaac tgtcagagcg gcgccggccc gggtggcgtc gcccccgagc gcaaggagga
     1981 acgcggccgc gagctcggcg gccgggacca ggatcccggc cgggcccgcg atcccgtccg
     2041 gcaggcggta tcccgccagc gcgcctcgcc aggccgagaa cctgccgagc ttggcgacgg
     2101 cagcccacac gaaggcggcg gcgagaagag tcgggacgac gagcacccgc ccgatcctac
     2161 gagcgtgtca ccgcaagtct gccctcctgg ggctgctcga cgcggccccc ggcatccccg
     2221 ggggcccgtg cgcgggcgat agcctggcta cgtggaaggt tggtgggccg tcagctacgt
     2281 cgtgatgtgg gtgttgctcg tcgccctgac cgtcgtcgtc gtggcactgg cccggcagat
     2341 cgggactctg cacctccggc tcggcccccg cggcgcgctc gcgctcgacg acgaagggcc
     2401 gcccctgggc gaggctccgc cgcacgagg
//
LOCUS       DASQDO010000111         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_929156, whole genome shotgun sequence.
ACCESSION   DASQDO010000111 DASQDO010000000
VERSION     DASQDO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929156"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VEV43_03375"
     CDS             complement(<1..202)
                     /locus_tag="VEV43_03375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYP22591.1"
                     /translation="MTALREHHDALVRGGRKLLVPYVMAGVPDRESFADAVAAVAGPA
                     DAVELGLPFSDPLMDGPVIAEAG"
     gene            complement(199..1350)
                     /gene="trpB"
                     /locus_tag="VEV43_03380"
     CDS             complement(199..1350)
                     /gene="trpB"
                     /locus_tag="VEV43_03380"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYP22592.1"
                     /translation="MKTSGRFGEFGGQYVPEALMPALDELERAWEAAREDETFRDQLH
                     SLLGSIVGRPTPLYEAERFSERAGRRVLLKREDLAHTGAHKINNTIGQVLLASRMGKT
                     RIIAETGAGQHGVATATAAAYFGLPCTVYMGATDVARQNLNVVRMEMLGATVVPVEAG
                     SATLKDAINEALRDWVTNVEDTHYVIGSVVGPHPYPELVASLQSVIGEEARRDALALT
                     GELPAEVVACVGGGSNAIGLFRGFYRDEGVALTGVEAAGEGLDRRHGASITRGAPGVL
                     HGARSMVLQDDQGLITEPHSISAGLDYPGVGPEHAWLAASGRARYEAVTDEEALAAFR
                     ALAETEGILPALEPSHALAYVLRSDGEGTVLVGLSGRGDKDVATIASVR"
     gene            complement(1347..>1938)
                     /locus_tag="VEV43_03385"
     CDS             complement(1347..>1938)
                     /locus_tag="VEV43_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HYP22593.1"
                     /translation="LKGPLDLDLDAARLAKTYADAGASAISVLTEPDGFKGSLEDMEA
                     ALGAGVPVLRKDFVVDPWQVMESRAAGADAILLIVRVVGDGLGPLLSAARSLGMDALV
                     EVHHEHELDDALAAGATLIGVNHRDLTTFEVDPERTAKLAPRMPAECTVVALSGVSTR
                     AEVEQLEAAGAAAVLIGESLVTAADPGAKIRELLSR"
BASE COUNT          262 a          722 c          682 g          272 t
ORIGIN      
        1 gtcccgcctc ggcgatgacc gggccgtcca tcaacggatc ggagaacggg aggccgagct
       61 cgaccgcgtc ggcgggaccc gccaccgcgg ccaccgcgtc ggcgaacgac tccctgtccg
      121 gcactcccgc catcacgtac gggacgagga gcttgcggcc tccgcggacg agcgcgtcgt
      181 ggtgttcccg cagagccgtc accgcacgga tgcgatcgtg gctacgtcct tgtcgccgcg
      241 accggacagt ccgaccagca ccgtcccctc gccgtcggag cgcagcacgt acgccagcgc
      301 gtgcgacggc tcgagggccg ggaggatccc ctcggtctcc gcgagcgccc ggaacgcggc
      361 cagcgcctct tcgtcggtga cggcctcgta gcgcgcccgg ccgctcgccg cgagccacgc
      421 gtgctcgggc ccgacgccgg ggtagtcgag ccccgcggag atcgagtgcg gctcggttat
      481 gagtccctgg tcgtcctgga ggaccatgct gcgcgctccg tgcaggacgc cgggggcacc
      541 gcgggtgatc gacgcgccgt ggcggcggtc gagcccctcc cccgcggcct cgacgcccgt
      601 cagcgcgacg ccctcgtcgc ggtagaagcc gcggaagagc ccgatcgcgt tcgagcctcc
      661 gccgacgcac gcgacgacct cggccgggag ctcaccggtg agagcgagag cgtcgcggcg
      721 cgcctcctcg ccgatcaccg actggagcga cgcgacgagc tcgggataag ggtgcggtcc
      781 cacgacggac ccgatcacgt agtgcgtgtc ctcgacgttc gtcacccagt cgcgcagtgc
      841 ctcgttgatc gcgtccttga gcgtcgccga gcccgcctcg accggcacga cggtcgcgcc
      901 gagcatctcc atccgcacga cgttgaggtt ctgccgcgcg acgtcggtcg cgcccatgta
      961 gacggtgcag ggaaggccga agtacgcggc cgcggtcgcg gtcgcgacgc cgtgctgccc
     1021 ggcccccgtc tccgcaatga tcctggtctt gcccatccgg gacgcgagca ggacctggcc
     1081 gatcgtgttg ttgatcttgt gagcgccggt gtgcgcgagg tcctcgcgct tgagcaatac
     1141 acgcctgccc gcgcgctcgg agaaccgttc ggcctcgtag aggggggtgg gacgcccgac
     1201 gatcgagccc agcagggaat ggagctggtc gcggaacgtc tcgtcctcgc gcgccgcctc
     1261 ccaggctcgt tcgagctcgt cgagcgcggg catgagggcc tcgggcacgt actgcccccc
     1321 gaactcgcca aagcgcccgc tcgtcttcat cggctgagga gctcccggat cttcgccccc
     1381 gggtccgccg ccgtgacgag cgactcccct atcaggaccg cggctgcgcc ggccgcttcg
     1441 agctgctcga cctcggccct cgtcgacacg cccgacagcg cgacgaccgt gcactcggcg
     1501 ggcatccggg gcgcgagctt cgccgtccgc tcgggatcga cctcgaacgt cgtcaggtcg
     1561 cggtggttga cgccgatcag agtcgcaccg gcggcgagcg cgtcgtcgag ctcgtgctcg
     1621 tgatggacct cgacgagcgc gtccatcccc agcgaccgcg cggcggacag cagggggccg
     1681 aggccgtctc cgacgacgcg gacgatcagc aggatcgcgt cggcgccggc ggcgcgcgac
     1741 tccatcacct gccacgggtc gacgacgaag tccttgcgca ggacggggac gcccgctccg
     1801 agcgccgcct ccatgtcctc cagcgagccc ttgaacccgt ccggctcggt gaggaccgag
     1861 atcgcgctcg cgccggcgtc cgcgtacgtc ttggcgagcc gggccgcgtc gaggtcgagg
     1921 tcgagagggc ccttcagc
//
LOCUS       DASQDO010000112         1912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_500973, whole genome shotgun sequence.
ACCESSION   DASQDO010000112 DASQDO010000000
VERSION     DASQDO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500973"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..710)
                     /locus_tag="VEV43_03390"
     CDS             complement(129..710)
                     /locus_tag="VEV43_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22594.1"
                     /translation="MKTLRGRIALAAAGLVALLGYAVVVDLGMHAGRVHRGVEVAGFD
                     VGGLTEIEAFEALEERRELLGETPVVFISPGFDCRVLPADPDGEDDPAAPDLGWSPQP
                     FNTALDAMEIGRGGIVEGLRERVDAWLGGYRIGWAGTVDPAKVDEFLDYCERNAQVVN
                     ATVDRPKLRFRIRRAIVTYPRSPLDFKVPLERD"
     gene            772..>1912
                     /locus_tag="VEV43_03395"
     CDS             772..>1912
                     /locus_tag="VEV43_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYP22595.1"
                     /translation="MGGRWAASDSLEGRLQRGRGLGFLDAVSDPGSASLVVRCVIEDP
                     RWDPQVEERGLYYARLIEKLGVALDPIEEHLLRDPQGPTLPLDTLEALGLHGDERALA
                     ILRRYLEEGAAWESALSRWANVEPPAALDDLRAIARRRGDESELTADPVVAAARIAPD
                     AARRREFPGLSTEELLERAAREPRLYVKFASALRDHSRAESRTTLLRTIATGDEGARA
                     AALVALGDLGDDGILDVCEELLRSGEPERLSRAAYRAATSLPTNGALERGRQWASATG
                     DLRTAGLVLVARLGAAPDLSLLQHTLVTASHEEDVYLLCDVADGLARIGDASSIEPLE
                     DAYERAVYSYLRGRCALALEMLSREFASTLAYESLWDCESTTRLAG"
BASE COUNT          276 a          682 c          687 g          267 t
ORIGIN      
        1 ccgagggaag aagagaacgc cgagcccctg tggacgccgg acccgaccga ggagccggag
       61 ccgacaccgg agccgagcga ggagcccgct ccgtcggagg agccggcccc gtccgagagc
      121 cccggcggct agtcccgctc gagcgggacc ttgaagtcga gcggcgagcg cgggtacgtg
      181 acgatcgccc gcctgatcct gaagcgcagc ttcggccggt cgaccgtcgc gttcacgacc
      241 tgcgcgttgc gctcgcaata gtcgaggaac tcgtccacct tcgccgggtc gaccgtcccg
      301 gcccacccga tgcggtagcc gccgagccac gcgtcgacgc gctcgcgcaa accctcgacg
      361 atcccgccgc gcccgatctc catcgcgtcc agcgcggtgt tgaacggctg cgggctccag
      421 ccgaggtcgg gggccgccgg gtcgtcttcg ccgtccggat ccgccgggag cacgcggcag
      481 tcgaaccccg gagagatgaa gacgaccggc gtctccccga gaagctcccg gcgctcctcc
      541 agggcttcga acgcctcgat ctcggtgagg ccgccgacgt cgaacccggc gacctcgaca
      601 ccccggtgga cgcggcccgc gtgcataccg aggtcgacga cgacggcgta gccgaggagc
      661 gcgacgagcc cggccgctgc gagcgcgatc cggccccgga gagtcttcat gcggccatgc
      721 taggagccgg ggcctgcctc ggcgtgcatc tcgcgcctct agagtcccgc catgggcggc
      781 cggtgggcgg catccgattc actggagggg cggcttcaac gcggccgcgg cctgggcttc
      841 ctcgacgccg tctcggaccc cggttccgcg tcactcgttg tgcggtgcgt gatcgaggac
      901 ccgcgctggg atccgcaggt cgaggaacgg ggcctgtact acgcccggct gatcgagaag
      961 ctcggcgtgg cgttggaccc catcgaggaa catctgctgc gcgatccgca ggggccgacg
     1021 ctgcctctcg acacgctcga ggcgctgggc cttcacggcg acgagcgcgc gcttgcgatc
     1081 ctgcggcgct atctggagga aggcgcggcg tgggagagcg cgctgtcccg gtgggcgaac
     1141 gtggagccgc cggccgcgct cgacgacctt cgcgcgatcg cgaggcggcg tggggacgag
     1201 tccgagctga ccgccgatcc cgtcgtcgcg gccgcacgca tcgctcccga cgcggcgcgg
     1261 cgtcgggagt tccccggact gtcgacggag gagctgctgg agcgcgcggc gcgggagccg
     1321 cggctctacg tgaagttcgc gtcggcgctt cgcgaccatt cgcgggccga gtcacgcacg
     1381 accctgctcc gcaccatcgc tacgggtgac gaaggagcaa gggccgcggc gttggttgcg
     1441 ctcggtgatc tcggcgacga cgggatcctc gacgtgtgcg aggagcttct ccgcagcgga
     1501 gagccggagc gcctgtcccg tgcggcgtat cgagcggcga cgtcccttcc gacgaacggc
     1561 gcgctcgagc ggggcaggca gtgggccagc gcaacaggcg acctgcgcac ggcggggctc
     1621 gtgctcgtag cgcgcctggg tgcggctcct gacctctcac tgctccagca cacgctcgta
     1681 actgcctcgc acgaagagga cgtctacctc ctgtgcgacg tcgccgacgg gctagcgcgc
     1741 atcggggacg cgagcagcat cgagcctctc gaagacgcct acgagcgcgc tgtctactcc
     1801 tacctgcgcg gtcggtgcgc tctcgcactc gaaatgctct cccgcgagtt cgcgtcgaca
     1861 ctcgcctacg aaagcctctg ggactgcgag tcgaccacgc ggctcgccgg at
//
LOCUS       DASQDO010000113        14745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_623758, whole genome shotgun sequence.
ACCESSION   DASQDO010000113 DASQDO010000000
VERSION     DASQDO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623758"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VEV43_03400"
     CDS             complement(<1..1373)
                     /locus_tag="VEV43_03400"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HYP22596.1"
                     /translation="MSQAARPPRRPVGEGQWGLGYSTPLNKPEQTKRDDDGLNVRKRI
                     EEVYSKKGFRSIWPDDLRSRFRWWGLYTQRTQGVPGGRTAAVEPHELEAEFFMLRVRI
                     AGGLLTSEQLRTIAWVSERFGRDVADVTDRQNVQLHWIRIEDVPRIFEAIESVGLTTC
                     EACGDTPRNMIGCPLAGVDRDEIIDPTPALLELSRRFVGDPAFSNLPRKYKTSMTGCV
                     QQCAQHEINDVSFLGTRAPDGRAGYDVWVGGGLGPNPKFARRLGVFVTPEEVADVWVG
                     ITSVFRDYGYRRARNRARLKFLMADWGPERFREVLQEKYLGRELEDGDAPPPSASSQR
                     DHLGVHEQKDGLHYAGFAPRAGRIFGHQLRHVADIADRYGSGRIRTTTHQKMVVLDIP
                     SDRVEEAVAELEDHDLQVRPSVFRAGTMACTGIEFCKLAIVETKQRADWLYRELEERL
                     PGFEQHIR"
     gene            complement(1370..2137)
                     /locus_tag="VEV43_03405"
     CDS             complement(1370..2137)
                     /locus_tag="VEV43_03405"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004949401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HYP22597.1"
                     /translation="MNPLAADPRVSEEFASPDVHFAELAEAPPQEILRWAFGTIGRVA
                     VATSFQSSGLVILHMTRALGLRPPVLFIDTGFHFPETIAFRRRIADEWDLDVVDVRGE
                     HGSAEGQAAIYGPALYERDPEKCCFINKVAPLQMALEGFDGWISGIRRDQSPLRAETP
                     TMEAQMLQSGKEVLKIHPLAHWSRDDVAAYVTEHAIPTHPLLEQGYRSIGCAPCTRAI
                     RPDEDERAGRWSGMTKTECGIHLFGKKNEPAQSEAEQ"
     gene            2358..2951
                     /gene="pyrR"
                     /locus_tag="VEV43_03410"
     CDS             2358..2951
                     /gene="pyrR"
                     /locus_tag="VEV43_03410"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HYP22598.1"
                     /translation="MPERAEAPAAAPGLEVKATVMEPDEVRRAVTRIAHEIVERNRGT
                     DDLVIVGIRTRGAPLAERIASAIASFEGVDLPSGALDVTLYRDDVALKSPRSLEATTV
                     PADVDGRVVVLVDDVLYTGRTIRAAFDAILDLGRPRAIRLAVLVDRGHREFPIRADHV
                     GKNLPTAADEVVKVHLQEIDGEDAVLIGHDGNRRASA"
     gene            2948..3880
                     /locus_tag="VEV43_03415"
     CDS             2948..3880
                     /locus_tag="VEV43_03415"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HYP22599.1"
                     /translation="MIKHLLSMDQLSADDITRILDTAESLRQVTDRPIKKLPTLRGRT
                     VCNLFYEASTRTRISFELAAKRLSADVINFSADSKSSVAKGESFKDTAWTIEAMGVDA
                     IVVRHGSSGAPHQLSRWVKASVLNAGDGAHEHPTQALLDLYSIRERFKDLDGLKVAIV
                     GDIIHSRVARSNVKGLVTMGADVVLVGPPTLVPPEHAAWGASVCYDLDQVIADVDVCY
                     LLRVQQERQSEQLFPSNREYAALWGLDSRRMAKMKPEALVMHPGPMNRGVEIAADVSE
                     SPRSIILDQVSNGLAVRMSLLYLMLGGQDEEVAA"
     gene            3877..5184
                     /locus_tag="VEV43_03420"
     CDS             3877..5184
                     /locus_tag="VEV43_03420"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYP22600.1"
                     /translation="MSDFVIRGGRVIDPASGRDEIADVKVEDGVVTAIGNGLDASEVV
                     DATNMVVAPGFVDMHTHLREPGREDEETVATASAGAAAGGFTAVCAMPNTDPVADSAS
                     VVEQVWSLGREAGLVEVTPAGALSKSLEGRKMADLGEMARSLAKVRLFTDDGHGVQDS
                     LFARRVMEYLVTFDAVYAEHCEDAALAAGGQMHEGSRSMALGLRGIPAAAEELMAARD
                     VALAKLTGCRLHLLHVSTRGTVEIVRRAKEDGVRVTAEATPHHFTLTDEALEGYDPLM
                     KVNPPLREDDDRRAIVEGLADGTIDAIATDHAPHAREEKDQEFAYAPPGMLGLETALG
                     LTITQLVEPGHLSLSRAIELLSTTPARILQMDGQGAIAEGRPANLVLFDPKARWTVEP
                     ERFQSFSRNTPFGGRELTGKVVHTFFRGRQTVRDGALLEGVRA"
     gene            5181..6365
                     /gene="carA"
                     /locus_tag="VEV43_03425"
     CDS             5181..6365
                     /gene="carA"
                     /locus_tag="VEV43_03425"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HYP22601.1"
                     /translation="MRALLALEDGTVFRGDAFGAEATVTGEACFNTGMTGYQEVLTDP
                     SYCGQIVTMTSPQIGNTGVNSFDDQSARPWVRGFAVRDLSSSVSSWRAEASLHEYLVG
                     HGIPGITGIDTRKLTRHLRTRGAMRSALSTVVTDASELVDIARSSPSMEGADLVVEVT
                     TNGAYEWTREKVEERRGSQFVAGAEAAATTVGLMSPAGRRYRIAAYDFGIKHNILDLL
                     VTAGCDITVVPAATSAGEVLDGGFDGVFLSNGPGDPAAVTYGIDAARGLVGRVPLFGI
                     CLGHQILGLALGAETFKLPFGHRGSNHPVKRVGRDRIEITCQNHGFAVSPGSIARTGG
                     HLTHENLNDGSVEGLEVPGVAFSVQYHPESGPGPHDSRYLFGAFRDLMESFEPADRPA
                     LV"
     gene            6483..6863
                     /locus_tag="VEV43_03430"
     CDS             6483..6863
                     /locus_tag="VEV43_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP22602.1"
                     /translation="MAAFDVVGLDHVEVTAPEELLDETCEWYRSCLELPELGKPEGTR
                     PVGAWFRAGDHEVHVSVDEHNPHKSAHFALVVTDFDAAVDRLRGSGCHLEQARSIPGR
                     HRCYTRDPAGNRIEIVSFDENVGA"
     gene            6863..10123
                     /gene="carB"
                     /locus_tag="VEV43_03435"
     CDS             6863..10123
                     /gene="carB"
                     /locus_tag="VEV43_03435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYP22603.1"
                     /translation="MPKRTDIRKILLIGSGPIVIGQASEFDYSGAQACKVLRQEGYEV
                     VLVNSNPATIMTDPEFADGTYVEPLTAAHVASIVAREKPDACLPTLGGQTALNLALEL
                     HESGVLEAHGVELIGARPESIRRAEDRRLFKETMIAAGLEVPKSTFAYSIDEAVAAAE
                     TVGYPLVVRPSFTLGGAGGGFVSDEDELRFVAAEGLRLSPIGELLIEESVYGWKEYEL
                     EVMRDGNDNVVVICSIENLDPMGVHTGDSITVAPAMTLTDVEYQAMRDDAAAVMRAIG
                     VETGGSNVQFAVNPADGRRVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITKKTPASFEPSIDYVVAKIPRWAFEKFPAADRTLGTKMKSVGEVMAIGRTFVE
                     ALQKGFRSLEVDLHGLGADGGREAPMPELRERLGQPTEDRLRLVEQALYQGMDEDEVV
                     KLSRIDPWFVHQIGRLAAARRELASLSPDALDADRLRDAKRMGFSDLQLARILRTTEP
                     EIRRRRIELGVVPTYKTVDTCAAEFEAHTPYYYSTYEEEDEVAPSTRPKVLILGSGPN
                     RIGQGIEFDYCCVHAAFALSDAGYETIMLNCNPETVSTDYDTSDRLYFEPLTFEDVMN
                     VVERERPAGVVVQLGGQTPLKLAGPLAAAGVPIWGTPFDAIDRAEDRERFGDLLADLA
                     LPHPANGIARSVAEARAVAASIGYPVLVRPSYVLGGRAMEIVYEESSLERYMSTAAEV
                     SPRHPVLVDRFLEGAVEVDCDAVFDGRELYVGGVMEHIEEAGIHSGDSACALPPFTLG
                     RDQVEQVVAHTEAIARSLGVRGLINMQFAVKDEQVFVIEANPRASRTVPFVSKATGVP
                     LAKVASRVMAGATLAQLRAEGMVPAKKIGHEGLPHVAVKEAVMPFDRFRGVDTLLGPE
                     MRSTGEVMGIDATFGLAFAKAEVGAGLRLPTKGAVFVSVANRDKRSVIFPAKRLADLG
                     FQLLATRGTAEVLRRAGVEVGIVEKVSSQEAGKNVADRIAAGEIDVVFNTPFGRGARS
                     DGYYIRTAAIQAGVPCITTMSGIAAAVQGIEALVTGQVGARSLQEYLADVDREVTA"
     gene            10124..11080
                     /locus_tag="VEV43_03440"
     CDS             10124..11080
                     /locus_tag="VEV43_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HYP22604.1"
                     /translation="MSSRTMAASTGEVLSHKKYGEAYHSLTIVAPDVGEKVRPGQFVN
                     VRCGEHRDHILRRPFSVYRVHKRGGWASTIEIVFDVRGPGTEYLSSLRGHSTVDLVGP
                     LGRGFAMPKRRAHCLLVGGGIGATPLFFLADELRNEGHRVDVILGARSTGLLLNAIEA
                     RRLASVCRTTTEDGSSGDRGRVTDILAETMEKCETEVVYSCGPHPMLAAVSRICVENK
                     VPVQVAVEELMGCGYGVCMTCVMPLRRPARKTTKGQEAEEDGVYYARSCTEGPVFNGA
                     SVVWNGSADAVAADQSELTPAGAPSRGTAALGAEDEHAPPGN"
     gene            11080..12027
                     /locus_tag="VEV43_03445"
     CDS             11080..12027
                     /locus_tag="VEV43_03445"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HYP22605.1"
                     /translation="MAEARKIRPDLSVKLAGAEFSNPVLVASGTFGAGREAAAFIDLA
                     APGGIIVKSMTLVPWRGKPTPRMCETPSGMLNAIGIQNKGVDHFVSEDLPWLLKRKAT
                     VVASIAGNTVEEFVRVADKLRTGGRGIAAVEINISCPNLEDRNNMFAHSTESTAAVVS
                     GVKNALKRIPVFPKLSPNVTSLPAIAEAAIDSGADGLSLVNTVFGMAIDVETKRPKLA
                     GVVGGLSGPAIRPIAVRAIHDVHRAWPHIPIIGQGGVTTATDALEMILAGASAVAVGT
                     GNFVNPRASLDVARGIGEYMVRHGIATVADLVGAVKLEG"
     gene            12024..12731
                     /gene="pyrF"
                     /locus_tag="VEV43_03450"
     CDS             12024..12731
                     /gene="pyrF"
                     /locus_tag="VEV43_03450"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYP22606.1"
                     /translation="MTATLRANPICVALDFDDPARVREIADATSDHVGIFKIGLTAYT
                     TGGPALVRDLTSRHPVFLDLKFHDIPAQVGGAVRAATATGAMYTTVHASGGPAMIAAA
                     VEAAGHSLTVLAVTVLTSLDDASLDSVGMKGSVRDSVLRLAGLALAAGADGLVCSPME
                     VAMLRSEFGARVDGGPFLVVPGIRAEGESHGDQKRTLSATEALAAGADVIVVGRPITA
                     APDPAGAARRLREQIGS"
     gene            12728..13309
                     /gene="pyrE"
                     /locus_tag="VEV43_03455"
     CDS             12728..13309
                     /gene="pyrE"
                     /locus_tag="VEV43_03455"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HYP22607.1"
                     /translation="MNPVEMMQVLEQKGAVLHGHFVLSSGRHSDLFVQKFRVLEHPRL
                     AQRFGESIAELYPGGFDLVACPAVGAVVLGFTTALAGGARMIFSERAEGAMAFRRGFE
                     VAPHERVLVVEDVVTTGGSAREVVDLVRAGGGTVVGVGALIDRSEPEDPPDLTVPLKA
                     LLKVPATSWAPEDCPLCARGEPVTDPGSRRLAR"
     gene            complement(13314..14135)
                     /locus_tag="VEV43_03460"
     CDS             complement(13314..14135)
                     /locus_tag="VEV43_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYP22608.1"
                     /translation="MLGTFECPPEAPAWNEENWVGAHPHVVFPRTAVLIERRGAEFVC
                     DPNTVVFYDANETFSRRLIDPSGDRCFYAELSEELVEEMLPGSAGAGRKLPGVTAPVD
                     ARVYALQWRTIRAFERDATAEWATALDEAILSVLGSIIAASASDAPGNDAGHRRIIES
                     TKRVISETLDQQLSLAKIAALVHVSPYHLTRLFRRYTGMPLHRYRMQLRLRGALERLE
                     GGRVPLREIAGSSGFASHSHFTARFRELFGFTPLQWRDERLDPCTLLALASGPAG"
     gene            complement(14318..>14745)
                     /locus_tag="VEV43_03465"
     CDS             complement(14318..>14745)
                     /locus_tag="VEV43_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22609.1"
                     /translation="VLEARRALGARGTGVDDGILAAGPRSVPEGPLADGDLTSEELTA
                     VMFRTATARPQGDAGDGPEPCPAEDPYGPAPVRWDSVPEDAPGYVSIGYGAIDGAATA
                     LALDVLGGRAPLPERPDEDAFFAQQETLRAAAHEVFTAL"
BASE COUNT         2237 a         5217 c         5171 g         2120 t
ORIGIN      
        1 cggatgtgct gctcgaaccc gggcaggcgc tcttcgagct cgcggtacag ccagtccgcg
       61 cgctgcttgg tctcgacgat cgcgagcttg cagaactcga tccccgtgca cgccatcgtc
      121 ccggcccgaa agacgctcgg ccgcacctgg aggtcgtggt cctcgagctc cgcgaccgct
      181 tcctcgacac ggtccgaggg gatgtcgagg acgaccatct tctggtgcgt cgtcgtcctg
      241 atccgtccgg agccgtaacg gtcggcgatg tccgcgacgt gccgcagctg gtggccgaag
      301 atccggcccg cccgcggcgc gaagccggcg tagtggaggc cgtccttctg ctcgtgcacg
      361 ccgaggtggt cccgctggga cgaagccgac ggcgggggag cgtcaccgtc ctcgagctcg
      421 cgcccgaggt atttctcctg cagcacctcg cggaaccgtt ccggccccca gtcggccatc
      481 aggaacttga gccgggcgcg gttgcgcgcc cgccggtagc cgtagtcacg gaacaccgac
      541 gtgatgccca cccagacgtc ggcgacctct tcgggagtga cgaagacgcc gagccgccgg
      601 gcgaacttcg ggttcggccc gagcccgcca ccgacccata cgtcgtaacc cgctcgcccg
      661 tccggcgcgc gcgtcccgag gaacgagacg tcgttgatct cgtgctgcgc gcactgctgc
      721 acgcacccgg tcatcgaggt cttgtacttg cgcggcaggt tcgagaacgc agggtcgccg
      781 acgaagcggc gcgagagctc gagcagtgcc ggcgtggggt cgatgatctc gtcgcggtcg
      841 acgccggcga gcgggcaccc gatcatgttg cgcggggtat cgccgcatgc ctcgcacgtc
      901 gtcagcccga ccgactcgat cgcttcgaag atgcgcggga cgtcctcgat gcggatccag
      961 tggagctgga cgttctgacg atcggtgacg tcggcgacgt cgcggccgaa gcgttcggac
     1021 acccacgcga tcgtccgcag ctgctcgctc gtcagcagtc cgccggcgat gcgcacgcgc
     1081 agcatgaaga actcggcctc gagctcgtgc ggctcgaccg ccgccgtccg gcccccgggc
     1141 actccctgag tccgctgcgt gtagagcccc caccaccgga accggctgcg gaggtcgtcc
     1201 ggccagatcg agcgaaaccc cttcttcgag tagacctcct cgatccgctt gcggacgttg
     1261 agcccgtcgt cgtcgcgctt cgtctgctcg ggcttgttga gcggggtcga gtagccgagg
     1321 ccccactggc cctcgccgac agggcgccgg ggcggccgcg ctgcctggct cactgttccg
     1381 cctcgctctg cgccggctcg ttcttcttgc cgaacaggtg gatcccgcac tccgtcttcg
     1441 tcatgcccga ccagcgcccg gcccgctcgt cctcgtccgg cctgatcgcc cgcgtgcacg
     1501 gcgcgcaccc gatcgatcgg tagccctgct cgagcagcgg gtgcgtcggg atcgcgtgct
     1561 cggtgacgta ggcagcgacg tcgtcgcggc tccagtgcgc gagcgggtgg atctttaaga
     1621 cctccttgcc gctctggagc atctgcgcct ccatcgtcgg cgtctcggcg cgcagcggcg
     1681 actggtcccg gcggatgccg gagatccatc cgtcgaagcc ctccagcgcc atctgcaggg
     1741 gagcgacctt gttgatgaag cagcacttct cggggtcgcg ctcgtacagg gccgggccgt
     1801 agatcgcggc ctgcccctcc gccgacccgt gctcgccgcg gacgtcgacg acgtcgaggt
     1861 cccactcgtc cgctatccgg cggcggaagg cgatcgtctc ggggaagtgg aagccggtgt
     1921 cgatgaagag gacgggcggg cggagcccga gggcccgggt catgtggagg atcacgaggc
     1981 cggaggattg gaacgaggtg gcgacggcga cgcggccgat ggtcccgaag gcccagcgca
     2041 ggatttcctg cgggggagcc tcggcgagct cggcgaaatg gacgtccggg gacgcgaact
     2101 cctcggagac acgcggatcg gcggcgagcg ggttcatctg gccgagcata ctattcgcct
     2161 atttccacgt ggtaaataga ccaatctcct agggaagggt gattgccctc gggaccgccg
     2221 gggtcgttga aggcccccgg tgcggtcgtg taccttagcc ccgcaatccg tcctttaacc
     2281 cggccccgtg aggccggaaa ggagccagcc ttgccctgtc cgaccctgca cgcctccgcg
     2341 accggaggcg ttttttgatg cccgagcgag ccgaggcccc cgcagcggcc ccgggcctcg
     2401 aggtcaaggc gaccgtcatg gagcccgacg aggtcaggcg ggccgtgacc cggatcgccc
     2461 acgagatcgt cgagcgcaac cggggcaccg acgacctcgt gatcgtcggg atccggaccc
     2521 ggggcgcccc cctcgccgag cgcatagcct ccgccatcgc ctccttcgag ggcgtcgacc
     2581 tcccctcggg cgcgctcgac gtcacgctct accgcgacga cgtcgcgctc aagagccccc
     2641 gcagcctcga agccaccacc gtcccggccg acgtcgacgg ccgcgtggtc gtgctcgtcg
     2701 acgacgtgct ctacaccgga cgcacgatcc gggcggcgtt cgacgcgatc ctcgacctcg
     2761 gccgtccccg cgcgatccgc ctcgcggtcc tggtcgaccg cgggcaccgt gagttcccga
     2821 tccgggcgga ccacgtcggc aagaacctcc cgaccgccgc agacgaggtc gtgaaggtgc
     2881 acctgcaaga gatagacggc gaggacgccg tactgatcgg gcacgacggc aaccggaggg
     2941 cctccgcatg atcaagcacc tgctctcgat ggaccagctg agcgcggacg acatcacgcg
     3001 catcctcgac accgcggagt cgctccgcca ggtcaccgac cgcccgataa agaagctccc
     3061 gacgctgcgg ggccggacgg tctgcaacct cttctacgaa gcgtcgacgc gcacgcggat
     3121 ctcgttcgag ctcgcggcga agcggctcag cgcggacgtg atcaacttct ccgccgactc
     3181 gaagtcctcg gtcgccaagg gtgagtcctt caaggacacc gcatggacga tcgaggcgat
     3241 gggcgtcgac gcgatcgtgg tgcgccacgg ctcgtcgggg gccccgcacc agctcagccg
     3301 ctgggtgaag gcgagcgtgc tgaacgcagg cgacggcgcc cacgagcacc cgacgcaggc
     3361 cctgctcgac ctctactcga tccgggagcg cttcaaggac ctcgacgggc tcaaggtcgc
     3421 gatcgtcggc gacatcatcc actcacgggt cgcgcgctcg aacgtgaagg ggctcgtgac
     3481 gatgggagcg gacgtcgtcc tcgtcggccc cccgaccctc gtgccgccgg agcacgctgc
     3541 gtggggcgcg agcgtctgct acgacctcga ccaggtgatc gccgacgtcg acgtctgcta
     3601 tctgctgcgg gtccagcagg agcgccagtc cgagcagctc ttcccgagca accgcgagta
     3661 cgcggccctc tggggcctcg actccagacg catggcgaag atgaagcccg aggcgctcgt
     3721 gatgcacccg ggcccgatga accgcggcgt cgagatcgcc gccgacgtgt cggagtcgcc
     3781 gcgctcgatc atcctcgacc aggtctcgaa cggtctcgcg gtccggatga gcctgctgta
     3841 cctgatgctc ggcggccagg acgaggaggt ggcggcatga gcgacttcgt catccgcggg
     3901 ggccgcgtga tcgacccggc gagcgggcgc gacgagatcg ccgacgtcaa ggtcgaggac
     3961 ggcgtcgtca ccgcgatcgg caacgggctc gacgcttccg aggtggtcga cgcgacgaac
     4021 atggtcgtcg cccccggctt cgtcgacatg cacacgcacc tgcgcgagcc gggccgcgag
     4081 gacgaagaga ccgtcgcgac cgcgagcgcg ggggccgcgg ccggcggctt caccgccgtg
     4141 tgcgcgatgc cgaacaccga cccggtggcg gactcggcct cggtcgtcga acaggtgtgg
     4201 tcgctgggcc gcgaagccgg gctcgtcgag gtcacgcccg ccggggctct cagcaagtcg
     4261 ctcgaaggac gcaagatggc cgacctcggc gagatggcgc gctcgctcgc gaaggtgcgc
     4321 ctgttcaccg acgacgggca cggcgtccag gactcgctgt tcgcccggcg ggtcatggag
     4381 tacctcgtga cgttcgacgc ggtgtacgcc gaacactgcg aggacgcggc cctcgccgcc
     4441 ggaggccaga tgcacgaggg ctcgcgctcg atggcactcg gtctgcgcgg catcccggcc
     4501 gcagccgagg agctgatggc ggcgcgcgac gtcgcgctcg cgaagctgac cggctgcagg
     4561 ctccacctcc tgcacgtctc gactcgcggg accgtcgaga tcgtccgccg cgcgaaggaa
     4621 gacggcgtgc gcgtgaccgc ggaggcgacg ccgcaccact tcacgctcac cgacgaggcg
     4681 ctcgagggct acgacccact gatgaaggtc aaccccccgc tgcgcgagga cgacgaccgc
     4741 cgcgcgatcg tcgagggcct ggccgacggg acgatcgacg cgatcgccac cgaccacgcg
     4801 ccgcacgcga gggaggagaa ggaccaggag ttcgcctacg ccccgccggg gatgctcggc
     4861 ctcgagaccg cgctgggatt gacgatcacg cagctcgtcg agcccggcca cctctcgctg
     4921 agccgggcga tcgagctgct gagcacgact cccgcgcgca tcctccagat ggacggccag
     4981 ggcgcgatcg ccgagggacg gcctgcgaac ctcgtcctgt tcgacccgaa ggcgcgctgg
     5041 acggtcgagc cggaacggtt ccagtcgttc agccgcaaca cgccgttcgg gggccgcgag
     5101 ctcaccggca aggtcgtcca cacgttcttc cgcggcagac agaccgtccg cgacggtgcc
     5161 ctcctcgaag gggtgagggc atgagggctc tgctcgcgct cgaggacggc acggtcttcc
     5221 gcggcgacgc gttcggcgcg gaggcgaccg tcaccggaga ggcctgcttc aacaccggga
     5281 tgaccggcta ccaggaggtc ctcaccgacc cgtcctactg cgggcagatc gtgacgatga
     5341 cgtcgccgca gatcggcaac accggcgtga actcgttcga cgaccagtcg gcgcggccct
     5401 gggtgcgagg cttcgccgta cgggacctca gctcgtcggt ctcgtcgtgg cgcgcggagg
     5461 cgagcctgca cgagtacctc gtcggacacg ggatccccgg gatcaccggc atcgacacgc
     5521 gcaagctcac gcgtcacttg aggacgcgcg gagcgatgcg aagcgcgctc tcgaccgtgg
     5581 tcaccgacgc tagcgagctc gtcgacatag cccgttcctc gccgtcgatg gaaggtgccg
     5641 acctcgtcgt cgaggtcact acgaacggcg cctacgagtg gacgcgcgag aaggtcgagg
     5701 agcgccgcgg ctcccagttc gtcgccgggg ccgaggcagc ggccacgacc gtcggcttga
     5761 tgagccccgc cgggcgccgc taccgcatcg ccgcatacga cttcgggatc aagcacaaca
     5821 tcctcgacct cctcgtgacg gcggggtgtg acatcaccgt ggttcccgcc gcgacctccg
     5881 cgggcgaagt cctcgacggg ggcttcgacg gtgtgttcct gtcgaacggg cccggcgacc
     5941 ccgccgccgt cacctacggg atcgacgcgg cccgcgggct cgtcggacgc gtccctctgt
     6001 tcggcatctg cctcggccac cagatcctcg gcctggcact cggggccgag acgttcaagc
     6061 tcccgttcgg ccaccgcggc agcaaccatc ccgtgaagag agtgggccga gaccgcatcg
     6121 agatcacgtg ccagaaccac ggcttcgcgg tgtcgcccgg atcgatcgcc cgcacgggcg
     6181 ggcacctgac acacgagaac ctcaacgacg ggtcggtcga gggcctcgag gttccgggcg
     6241 tcgccttcag cgtccagtac cacccggagt ccggccccgg cccccacgac tcgcggtatc
     6301 tgttcggggc gttccgtgac ctgatggaat cgttcgagcc ggccgaccgg ccggcgcttg
     6361 tctagaaagg agtagccatg gggcgaggtg acaggaagag agcccgctgg aagaaggacc
     6421 gccagcgtcg caagaagtcg cgcgagaagc gccgcgcggc cgcagccggg gcgaaccgct
     6481 aagtggccgc tttcgacgtc gtcggtctcg accacgtcga ggtgacggcg cccgaggagc
     6541 tcctggacga gacctgcgag tggtaccgga gctgcctcga gctgccggag ctcggcaagc
     6601 ccgaagggac gcggccggtg ggcgcgtggt tccgcgcggg cgaccacgag gtgcacgtgt
     6661 cggtcgacga gcacaacccg cacaagagcg cgcacttcgc tctcgtcgtc accgacttcg
     6721 acgcggcggt cgaccggctg cgcggctccg ggtgccacct cgagcaggcc aggtcgatcc
     6781 cgggccggca ccgctgctac acgcgtgacc ccgccgggaa ccgcatagag atcgtttcct
     6841 tcgacgagaa cgtgggcgcc tgatgccgaa gcgcactgac atccgcaaga tcctcctcat
     6901 cggctccggc ccgatcgtca tcggccaggc ctccgagttc gactactccg gcgcccaggc
     6961 gtgcaaggtc ctgcgccagg agggctacga ggtcgtgctc gtgaacagca acccggcgac
     7021 gatcatgacc gaccccgagt tcgccgacgg gacctacgtc gagccgctca cggccgctca
     7081 cgtcgcctcg atcgtcgctc gcgagaagcc cgacgcatgc ctgccgacgc tgggcggcca
     7141 gaccgcgctg aacctcgcgt tagagctcca cgagtccggg gtcctggagg cgcacggcgt
     7201 cgagctcatc ggagcgcggc ccgagtccat ccgccgggcc gaggaccgca ggctcttcaa
     7261 ggagacgatg atcgccgcgg ggctggaggt cccgaagtcg acgttcgcgt actcgatcga
     7321 cgaagccgtc gccgcagccg aaaccgtcgg ctacccgctc gtcgtccggc cctcgttcac
     7381 gctcggcggc gccggcgggg gattcgtctc ggacgaggac gagctgcgct tcgtagcggc
     7441 cgagggtctc cggctctcac cgatcggcga gctcctcatc gaggagtccg tctacggctg
     7501 gaaggagtac gagctcgagg tcatgcgcga cggcaacgac aacgtcgtgg tgatctgctc
     7561 gatcgagaac ctcgacccca tgggcgtgca caccggcgac tcgatcacgg tcgcgccggc
     7621 gatgaccttg accgacgtcg agtaccaggc catgcgcgac gacgccgccg cggtcatgcg
     7681 cgctatcggc gtcgagaccg gggggtcgaa cgtccagttc gccgtgaacc ccgccgacgg
     7741 ccgccgcgtc gtgatcgaga tgaacccgcg cgtgtcgcgc tcgtccgccc tcgcgtcgaa
     7801 ggcgaccggg ttccccatcg cgaagatcgc ggcgaagctc gccgtcggct acacgctcga
     7861 cgagatcccc aacgacatca cgaagaagac cccggcgtcc ttcgagccct cgatcgacta
     7921 cgtcgtggcg aagatcccgc gctgggcctt cgagaagttc ccggccgcgg accgcacgct
     7981 cggcacgaag atgaagtccg tcggcgaggt catggcgatc gggcgcacgt tcgtcgaagc
     8041 cctccagaag ggcttccggt cgctagaggt cgacctgcac gggctcggcg ccgacggggg
     8101 ccgcgaggcc ccgatgccgg agctgcgcga acgcctcgga cagcccacgg aggaccgcct
     8161 gcggctcgtc gagcaggcgc tctaccaggg catggacgaa gacgaggtgg tgaagctgtc
     8221 gcgcatcgac ccgtggttcg tccaccagat cggacgcctc gccgccgctc gccgtgagct
     8281 cgcgtcgctc tcgcccgacg ctctcgacgc ggaccgcctg cgcgacgcga agcgcatggg
     8341 cttctccgac ctccagctcg cgcggatcct gcgcacgacc gagcccgaga tacggcgtcg
     8401 caggatcgag ctcggcgtcg tcccgaccta caagaccgtc gacacgtgcg ccgccgagtt
     8461 cgaggctcac acgccgtact actactcgac ctacgaagaa gaggacgagg tcgcgccgtc
     8521 gacgcgaccg aaggtcctga tcctcggctc cggcccgaac cgcatcggcc aagggatcga
     8581 gttcgactac tgctgcgtgc acgcggcatt cgcgctgtcc gacgccggct acgagaccat
     8641 catgctgaac tgcaaccccg agacggtctc gaccgactac gacacctcgg atcgcctcta
     8701 cttcgagccg ctgacgttcg aagacgtcat gaacgtcgtc gaacgcgagc gcccggcggg
     8761 cgtcgtcgtc cagctcgggg gacagacgcc gctcaagctc gccggccccc tcgccgccgc
     8821 cggagtcccg atctggggga cgcccttcga cgcgatcgac cgggccgagg accgcgagcg
     8881 cttcggcgac ctgctcgccg acctcgcgct cccgcatccg gcgaacggga tcgcgcgctc
     8941 ggtggcggag gcccgcgccg tggccgcttc gatcgggtac ccggtcctcg tgcggccgag
     9001 ctacgtgctc gggggccgcg ccatggagat cgtctacgag gagtcgtcgc tcgagcgtta
     9061 catgtcgacg gcggcggagg tctcgccccg tcacccggtt ctagtcgacc gcttcctcga
     9121 aggcgccgtc gaggtcgact gcgacgcggt cttcgacggg cgcgagctct acgtcggcgg
     9181 cgtgatggag cacatcgagg aggcagggat ccactccggc gactcggcgt gcgccctgcc
     9241 cccgttcacg ctcggccggg accaggtcga acaggtcgtc gcgcacacgg aggcgatcgc
     9301 ccggagcctc ggcgtccggg gtctgatcaa catgcagttc gccgtcaagg acgagcaggt
     9361 gttcgtcatc gaggcgaacc cgcgcgcgtc gcggacggtc ccgttcgtct cgaaggcgac
     9421 aggcgtcccg ctggcgaagg tcgcgtcgcg cgtcatggcc ggcgccacgc tggcgcagct
     9481 gcgcgccgaa gggatggtcc ccgccaagaa gatcggtcac gagggcctcc cgcacgtcgc
     9541 ggtgaaagag gccgtcatgc ccttcgaccg cttccgcggc gtcgacacct tgctcggccc
     9601 cgagatgcgc tcgaccggcg aggtcatggg catcgacgcg acgttcggcc tcgcgttcgc
     9661 gaaggccgag gtcggcgcgg gcctgaggct cccgacgaag ggcgcggtct tcgtctcggt
     9721 cgcgaaccgg gacaagcgct cggtgatctt cccggcgaag cgtcttgcgg acctcggctt
     9781 ccagctcctc gcgacgcgag ggacggccga ggtgctcaga agggccgggg tcgaggtcgg
     9841 gatcgtcgag aaggtctcgt cccaggaggc gggcaagaac gtcgccgacc ggatcgccgc
     9901 cggcgagatc gacgtcgtgt tcaacacgcc gttcggccgg ggggcgcgct ccgacggcta
     9961 ctacatcagg accgccgcga tccaggccgg cgtgccgtgc atcacgacga tgtcgggcat
    10021 cgcggccgcg gtgcagggga tcgaggcgct ggtgacgggc caggtcggcg ctcgctcgct
    10081 ccaggagtac cttgcggacg tggaccgcga ggtgactgca tagatgtcct cccgcacgat
    10141 ggcggcgtcg accggcgagg tcttgtcgca caagaagtac ggcgaggcct accactcgtt
    10201 gacgatcgtg gcccccgacg tcggcgagaa ggtgcgcccg ggccagttcg tcaacgtccg
    10261 ctgcggagag caccgcgatc acatcctcag gcggccgttc tccgtctacc gcgtgcacaa
    10321 gcgcggcggc tgggcgtcga cgatcgagat cgtcttcgac gtaaggggac cgggcaccga
    10381 gtacctgtcg agccttcgcg gccactcgac cgtagacctc gtcggcccgc tcggacgcgg
    10441 cttcgccatg ccgaagcggc gggcccattg cctgttggtg ggcggaggga tcggcgcgac
    10501 gccgctgttc ttcctcgccg acgagcttcg caacgaaggc caccgcgtcg acgtcatcct
    10561 cggcgcccgg agcaccggcc tgctgctgaa cgcgatcgag gcgcgccggc tcgcgagcgt
    10621 gtgccggacg acgaccgagg acggttcctc cggcgaccgc gggagggtca ccgacatcct
    10681 ggccgagacc atggagaagt gcgagaccga ggtcgtgtac tcctgcggcc cccacccgat
    10741 gctcgcggcg gtgtcgcgca tctgcgtcga gaacaaggtg ccggtgcagg tcgcggtcga
    10801 agagctgatg ggctgcggct acggcgtctg catgacgtgc gtgatgccgc tgcgccgccc
    10861 cgcacgcaag acgacgaagg gccaggaggc cgaagaggac ggcgtctact acgcccggtc
    10921 ctgcacggaa ggcccggtgt tcaacggcgc gtcggtcgtg tggaacggga gcgcggacgc
    10981 ggtcgcggcc gaccagtcgg agctgacccc cgccggggcg ccctcgcgcg gcaccgccgc
    11041 gctcggcgcc gaagacgagc acgcaccgcc gggcaactga tggcggaggc gaggaagatc
    11101 cggcccgacc tctcggtcaa gctcgcgggc gccgagttct cgaaccccgt cctcgtcgcg
    11161 tccgggacgt tcggcgcggg acgcgaggcg gcggcgttca tcgacctcgc cgcgccgggc
    11221 gggatcatcg tgaagtcgat gacgctcgtg ccgtggaggg gcaagcccac gccgcgcatg
    11281 tgcgagacgc cgtcggggat gctcaacgcc atcggcatcc agaacaaggg tgtcgaccac
    11341 ttcgtctccg aggacctgcc gtggctgctc aagcgcaagg ccacggtcgt cgcgtcgatc
    11401 gccggcaaca ccgtcgagga gttcgtgcgc gtcgccgaca agctccggac cgggggccgc
    11461 gggatcgcgg cagtcgagat caacatctcg tgcccgaacc tcgaggaccg caacaacatg
    11521 ttcgcccact cgaccgagtc gacggccgcg gtcgtttccg gcgtgaagaa cgctctcaag
    11581 cggattcccg tgttcccgaa gctctccccg aacgtgacgt cgcttccggc catagcggag
    11641 gccgcgatcg actccggcgc cgacgggctc tcgctcgtca acaccgtgtt cgggatggcg
    11701 atcgacgtcg agacgaagcg gccgaagctg gccggcgtcg tcggcggact ctcggggccc
    11761 gccatccggc cgatcgcggt gcgtgcgatc cacgacgtgc accgcgcctg gccgcacatc
    11821 ccgatcatcg ggcagggcgg cgtcacgacc gccaccgacg cgctcgagat gatcctcgcc
    11881 ggcgcctccg ccgtcgcggt cgggacgggg aacttcgtca acccgcgggc gtcgctcgac
    11941 gtcgcgcgag ggatcggcga gtacatggtc cgccacggca tcgcgaccgt ggccgacctc
    12001 gtgggcgcgg tgaagctcga gggatgacgg ccacgctgcg cgccaacccc atctgcgtcg
    12061 cgctggactt cgacgatccc gcgagggtgc gcgagatcgc agacgcgact tcggaccacg
    12121 tcgggatctt caagatcggt ctcaccgcgt acacgaccgg gggcccggcc ctcgtgcgcg
    12181 acctgacctc gcgccacccc gtgttcctcg acctcaagtt ccacgacatc cccgcgcagg
    12241 tcggcggcgc ggtccgggcg gcgaccgcga ccggcgcgat gtacacgacc gtccacgctt
    12301 ccggcggccc ggcgatgatc gccgcggcgg tcgaggcggc cgggcactcg ctcacggtcc
    12361 tcgcggtgac cgtgctgacg agcctggacg acgcctcgct cgactccgtc gggatgaagg
    12421 gctcggtacg cgactcggtc cttcgcctcg cgggcctcgc gctcgcggcc ggcgccgacg
    12481 ggctcgtgtg ctcgccgatg gaggtcgcga tgctccgctc cgagttcggc gcgcgggtcg
    12541 acgggggacc gttcctcgtc gtccccggca tccgcgccga gggcgagtcc cacggcgacc
    12601 agaagaggac gctgtccgcg accgaagcgc tcgccgccgg ggccgacgtg atcgtcgtcg
    12661 gccggccgat caccgcggcc cccgaccccg ccggcgcggc ccgccggctc cgggagcaga
    12721 tcggctcgtg aacccggtcg agatgatgca ggtgctcgag cagaaggggg ccgtgctcca
    12781 cggccacttc gtgctctcgt ccggacgtca ctccgacctg ttcgtccaga agttcagggt
    12841 gctcgagcac ccgaggctcg cgcagcgctt cggcgagtcg atcgccgagt tgtacccggg
    12901 cgggttcgac ctcgtcgcgt gtcccgcggt gggggcggtc gtcctcggct tcacgacggc
    12961 gctcgccggc ggggcgcgga tgatcttctc ggagagggcc gagggcgcga tggcgttccg
    13021 gcgcggcttc gaggtcgcgc cgcacgagcg cgtgctcgtc gtcgaggacg tcgtcacgac
    13081 ggggggctcg gcccgcgagg tcgtcgacct ggttcgggcc ggcggcggga ccgtcgtcgg
    13141 ggtcggggct ttgatcgacc gcagcgagcc cgaggacccg ccggacctga ccgtgcccct
    13201 gaaggccctg ctgaaggtcc cggcgacgtc gtgggccccc gaagactgcc cgctctgcgc
    13261 ccgcggcgag cccgtgacgg accccggcag ccgccgcctc gctcgctgaa cggctagccg
    13321 gcgggccccg aggccagagc aaggagcgtg caggggtcca gccgttcgtc gcgccactgc
    13381 aggggggtga agccgaacag ctcgcggaac cgcgcggtga agtggctgtg actggcgaaa
    13441 ccggacgagc cggcgatctc tctcagcggt acgcgccccc cctccaacct ctcgagagct
    13501 ccgcgtagcc ggagctgcat ccggtaccgg tggagcggca tcccggtgta ccggcggaac
    13561 agacgggtga ggtggtacgg agacacgtgg accaacgccg cgatcttcgc gagggacagc
    13621 tgttggtcca gggtctcgct gatcacgcgt ttggtgctct cgatgatgcg gcgatgcccg
    13681 gcgtcgttcc cgggggcgtc ggaagcggac gccgcgatga tcgagcccaa caccgagagg
    13741 atcgcttcgt ccagcgccgt cgcccactcg gccgtcgcgt ccctctcgaa cgcacggatc
    13801 gtccgccact ggagcgcgta cacccgcgcg tcgacggggg cggtgacccc ggggagcttc
    13861 cggcctgctc ccgcggaacc cggcaacatc tcctcgacga gctcctcgga gagctcggcg
    13921 tagaagcacc ggtcgccgct cgggtctatg agccggcgcg agaacgtctc gttcgcgtcg
    13981 tagaagacca cggtgttggg atcgcagacg aactcggcgc cgcgccgctc tatgaggacc
    14041 gccgtgcggg ggaagaccac gtgcgggtgc gcgccgaccc agttctcctc gttccacgcc
    14101 ggggcctccg gagggcactc gaacgtgccg agcacgaagc ccgggcctcc gtggacctct
    14161 gtcgcccaag gggacggtct cgagtcgggg ggttccatgg ctccctcacg cgcgtcggac
    14221 gaaggctgca cttccgtcta cgtgaggccg agcaccgggc aacgcgcgcg ggaaggacct
    14281 tcgagcggat cgagcaattc cgtgacaggc gcgcggctca gagggccgtg aagacctcgt
    14341 gagcggcggc ccgcagggtc tcctgctgcg cgaagaacgc gtcttcgtcg gggcgctccg
    14401 gcaggggagc ccggcccccg aggacgtcga gcgccagcgc ggtcgcggcc ccgtcgatcg
    14461 cgccgtaacc gatcgagacg taccccggcg cgtcctcggg caccgagtcc cacctcaccg
    14521 gcgccggccc atagggatcc tcggcgggac acggctccgg cccgtcgccc gcgtcgccct
    14581 gcgggcgagc ggtggcggtc cggaacatga cggcggtgag ctcctcggac gtgaggtccc
    14641 cgtcggccag cggtccctcc gggacggagc ggggcccggc ggccaggatg ccgtcgtcga
    14701 ctcccgtgcc gcgcgcgccg agagcgcgtc gggcttcgag gacga
//
LOCUS       DASQDO010000114         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_746199, whole genome shotgun sequence.
ACCESSION   DASQDO010000114 DASQDO010000000
VERSION     DASQDO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746199"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VEV43_03470"
     CDS             <1..932
                     /locus_tag="VEV43_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22610.1"
                     /translation="DVVVGTGIDRVEAGTGDDTIHDVDNFDCRDAGCSREPDDTFDGG
                     AGNDWILYEGSLGVTVDLTNGTATGAGADAVTELENVVGTEQADTILGDEGPNHLRSG
                     GGGDYVEGRGGDDELVDAYNHGGVSDGDEIHGGEGDDRLSSLSGTSSLHGEDGDDRIL
                     GGAGADAIEGGAGNDVVVGSGADDTIDGGDGSDTIDFTNTFLKVNLDLASGTGEKLIG
                     SFDTLFGTDAYVSIENATGSDKNDTLRGDDGPNALSGGPGSDSIEGRSGDDVIDAGEG
                     MEPGSSATERDVADGGPGTDTCTNAEEATGCEA"
     gene            953..1282
                     /locus_tag="VEV43_03475"
     CDS             953..1282
                     /locus_tag="VEV43_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22611.1"
                     /translation="MLIRYVMWELDGTATTIEELREYLRDESVDAFSEVRGLRFKAWF
                     SDAATNRWGAVYVWEDAEAAAQKVPSRARELIGKDPDVQEVFEVEATAEGVFEHAGLT
                     RRGLAFE"
     gene            complement(1287..1763)
                     /locus_tag="VEV43_03480"
     CDS             complement(1287..1763)
                     /locus_tag="VEV43_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYP22612.1"
                     /translation="MNWEEVTAAAPELAAKVRARFEAHGLAFLATLRKDGSPRISGIE
                     TLFGEGELWLGAMPGSLKAKDLLRDPRLALHSASTDKMVSEGDAKLSGLVVHVEDEAT
                     FERYKKASGGAPGLSLGGFHLFRVDVKEMSHLATAGDHLDIEWWTEAGGYRQKDRY"
     gene            1892..2494
                     /locus_tag="VEV43_03485"
     CDS             1892..2494
                     /locus_tag="VEV43_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22613.1"
                     /translation="MFAQIVQGRVTDPAGVRKQWDKWVEDVKPGAQGFLGSTGGVTAG
                     GQFITIARFENEEAARANSDRPEQSAWWEETSQYVEDPMFHDCTLVDVFGGGGSDDAG
                     FVQVIQGKTKDVEKSRDLDARMEPGMRDRRPDVIGGVNAWHPDNGRFTTFIYFTSEAE
                     ARAKEKEMANDESFGEYMQEMQELSDGEPKFLDLSEPWMA"
     gene            2499..>3008
                     /locus_tag="VEV43_03490"
     CDS             2499..>3008
                     /locus_tag="VEV43_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HYP22614.1"
                     /translation="MADVPGLYERAATHFGALLREVRADQWNDATPCVGWTVRDLANH
                     DLGENRWVPPLLAGRTVAEVGDAFDGDLLGDDPAAAWDEAAAEALAAARGPAALERTV
                     HLSFGDHSGRYYIGQVLSDHVIHAWDLARAIGADEQQDAGLVRFAYDELVPQFEQWRS
                     AGAFGPRVEV"
BASE COUNT          500 a          989 c         1113 g          406 t
ORIGIN      
        1 acgacgtcgt cgtagggacg gggatcgatc gcgtcgaggc cggcacgggt gacgacacga
       61 tccacgacgt cgacaacttc gactgccgcg acgcggggtg ctcgagggag cccgacgaca
      121 cgttcgacgg cggtgcgggg aacgactgga tcttgtacga ggggtctctc ggggtgaccg
      181 tcgacctgac gaacgggacc gcgacggggg cgggcgcgga tgcggtcacc gagctcgaga
      241 acgtcgtcgg cacggagcag gccgacacga tcctgggaga cgaggggccg aaccacctcc
      301 ggtcgggcgg cggcggcgac tacgtcgagg gccgcggcgg cgacgacgag ctcgtcgacg
      361 cgtacaacca cggcggggtc tccgacggcg acgagatcca cggcggcgag ggtgacgacc
      421 ggctctcgtc gttgagcggc accagctcgc tccacggcga ggacggtgac gaccggatcc
      481 tgggcggagc cggggccgac gcgatcgaag ggggtgccgg gaacgacgtc gtcgtcggca
      541 gcggagccga cgacacgatc gacggtggcg acggatcgga cacgatcgac ttcacgaaca
      601 cgttcctgaa ggtgaacctg gacctcgcga gcggtacggg cgagaagctg atcggatcct
      661 tcgacaccct gttcggcacc gacgcctacg tctcgataga gaacgcgacc ggcagcgaca
      721 agaacgacac gctgcgcgga gacgacgggc cgaacgcgtt gtcgggaggg ccgggcagcg
      781 actcgatcga gggccgctcg ggcgacgacg tcatcgacgc cggcgagggc atggagcccg
      841 gcagcagcgc gaccgagcgc gacgtcgccg acggaggccc cgggaccgac acgtgcacga
      901 acgccgagga ggctacgggc tgcgaggcct gagccgcggc tacgctcctc ccatgctgat
      961 ccgctacgtg atgtgggagc tcgacggcac ggcgacgacg atcgaggagt tgcgcgaata
     1021 cctgcgcgac gagtccgtcg acgcgttctc ggaggtgcgc gggctccgct tcaaggcgtg
     1081 gttctcggac gccgcgacga accgctgggg cgcggtctac gtgtgggagg acgccgaggc
     1141 cgcggcccag aaggtgccga gccgtgcccg cgagctcatc gggaaggacc ccgacgtgca
     1201 agaggtcttc gaggtcgagg cgacggccga aggggtgttc gagcacgcgg ggctgacgcg
     1261 ccgcgggctc gcgttcgagt gacgcgtcag taacggtcct tctgccggta gccgccggcc
     1321 tcggtccacc actcgatgtc gaggtggtcg ccggccgtgg ccaggtgcga catctccttc
     1381 acgtcgacgc ggaagaggtg gaagcccccg aggctcagcc ccggcgctcc ccccgacgcc
     1441 ttcttgtagc gctcgaacgt ggcctcgtcc tcgacgtgca ccacgagccc cgagagcttc
     1501 gcgtccccct cgctcaccat cttgtccgtg ctcgcgctgt ggagcgcgag ccgcgggtcg
     1561 cgcagcaggt ccttcgcctt gagagagccg ggcatcgcgc cgagccacag ctcgccttcg
     1621 ccgaagagcg tctcgatgcc cgagatccgc ggcgacccgt ccttgcggag cgtcgcgagg
     1681 aacgcgagcc cgtgcgcctc gaacctggcc cggaccttcg ccgcgagctc gggggccgcg
     1741 gccgtgacct cctcccagtt catgtggtgc cctccttggc cggaacacgg gtcttcgtaa
     1801 ttagaacaat ccacggctgg tcaacttgac gcctcctctc aggggagtag aaccaagtcc
     1861 aggtcgatcg tcgagaagag gaggtaggcc tgtgttcgct cagatcgtgc agggacgggt
     1921 gaccgatccc gccggggtcc gcaagcagtg ggacaagtgg gtcgaggacg tgaagccggg
     1981 ggcccagggc ttcctcggct cgaccggcgg cgtcaccgcc ggcggccagt tcatcaccat
     2041 cgcccgcttc gagaacgagg aggcggcccg cgcgaacagc gaccgccccg agcagagcgc
     2101 gtggtgggag gagacctcgc agtacgtcga ggacccgatg ttccacgact gcaccctcgt
     2161 ggacgtgttc ggcggaggcg ggtccgacga cgcgggcttc gtgcaggtga tccagggcaa
     2221 gaccaaggac gtcgagaagt cccgcgacct cgacgcgcgc atggagccgg ggatgcgcga
     2281 ccgccgtccc gacgtcatcg gcggcgtcaa cgcgtggcac cccgacaacg gtcgcttcac
     2341 gacgttcatc tacttcacgt ccgaggccga ggcgagggcc aaggagaagg agatggcgaa
     2401 cgacgagtcg ttcggcgagt acatgcagga gatgcaggag ctgtccgacg gggagccgaa
     2461 gttcctggac ctctccgagc cctggatggc ctaggcactt ggccgacgtc cccggcctct
     2521 acgagcgagc ggctacgcac ttcggcgcgt tgcttcgtga ggtgcgcgcc gaccagtgga
     2581 acgacgcgac gccgtgcgtc ggctggacgg tgcgcgacct cgcgaaccac gacctcggcg
     2641 agaaccgctg ggtcccgccg ttgctcgcag gccggacggt agcggaggtc ggggacgcgt
     2701 tcgacggcga cctgctcgga gacgacccgg cggcggcatg ggacgaggcc gccgccgagg
     2761 cgctcgcggc ggctcggggg ccggctgcac tcgagcggac cgtgcacctg tcgttcggcg
     2821 accactccgg ccgctactac atcggccagg tcctgagcga ccacgtgatc cacgcatggg
     2881 acctggcccg cgcgatcggg gcggacgagc agcaggacgc cgggctggtg cgcttcgcct
     2941 acgacgagct cgtgccgcag ttcgagcagt ggcgttccgc aggggccttc ggcccccgcg
     3001 tagaggta
//
LOCUS       DASQDO010000115        10645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_134443, whole genome shotgun sequence.
ACCESSION   DASQDO010000115 DASQDO010000000
VERSION     DASQDO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134443"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /gene="gcvPB"
                     /locus_tag="VEV43_03495"
     CDS             complement(<1..1188)
                     /gene="gcvPB"
                     /locus_tag="VEV43_03495"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HYP22615.1"
                     /translation="MSTLEQSAGTRAGEDGSTPIFERSRPGRRSWSLPDPGDGAVEDL
                     IPSSFLRSAPPVLPEVSERDLVSHYTRLSQRNWAIDVGAYPLGSCTMKYNPKLAEDAA
                     ALEGFRALHPSADDELAQGALELLGILQDALCEATGMAALTLQPAAGAQGELTGLLIM
                     RAFHASRGETRHRVIIPDSAHGTNPASVSLAGFTAQEVPSDDRGLVDLDALREVLDDD
                     VAGLMLTNPNTLGLFERDIQEIAAALHEVGGLLYYDGANFNSIVGVVRPGDMGFDIVH
                     LNLHKTFATPHGGGGPGSGPLAVSERLARFLPAPVLVHDEGAGRWRWDDDRPDSIGRI
                     HSWHGNFGINVRAYAYLRCLGPEGLKRVAERAVLNANYLRELISDSFPTAFQGTCMHE
                     FVAT"
     gene            complement(1185..2525)
                     /gene="gcvPA"
                     /locus_tag="VEV43_03500"
     CDS             complement(1185..2525)
                     /gene="gcvPA"
                     /locus_tag="VEV43_03500"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HYP22616.1"
                     /translation="MDFIPHTPEDDREMLEAVGLGSLDELFESVPEAVRLARLLDVPE
                     SLSEIDLAGHMSELAAKSTGAGLVCFAGGGAYDHYVPAAVRALASRGEFATSYTPYQP
                     ELSQGVLQALFEFQTMVCELYGMEVANASLYDGANALVEGVNLAVRATKREVVFVAET
                     VHPHYVGVLRSYTSGLGLDIRSVPKTGAGAIDWAAHDLSGAAAVVVQSPSYFGLLEDT
                     GAAATAAHDAGALLVSVADPTAMGLLESPGAQGADVAVGEGQALGNSLSFGGPYVGLF
                     ATKLSLVRQVPGRIAGETVDADGRRAYVLTLQAREQHIRRAKATSNVCTNQTLMAIAA
                     TIYLSWLGPEGLRRLGELCVKRTALAAERLAALPGCRLAFDGPRFKELVLETPIDGRD
                     LAKRLARRGYLTGPSLGTHYEGMEKSLIVAVTERRTEAEIDGLAEAIEKELAEG"
     gene            complement(2554..3621)
                     /gene="gcvT"
                     /locus_tag="VEV43_03505"
     CDS             complement(2554..3621)
                     /gene="gcvT"
                     /locus_tag="VEV43_03505"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYP22617.1"
                     /translation="MTDRATPLLAAHQALGARLTEFAGWQMPLQYEGVIAEHNSVRTS
                     AGLFDVTHLGKLRVKGGGDALQRALTADVVGLEPGRATYALALVDDGGCVDDVFVYRI
                     ASDEWLVVPNAANVHAVAECIREAGGEPVDEWDRFAILALQGPDSFTVFEKVWPGAEA
                     LTLKLHHWCSLDVFGSAGLVARTGYTGERGFEIYAPFEVAERAWTALLDAGAAPVGLG
                     ARDTLRLEMGYALYGHELSREINPLEAGLGWALAWDTPFRGREALLAIKEGGGPARRL
                     FGIVCTDKGVPRQGYGVFTGDERVGELTSGNFSPTLGTGIALALGPAATRPEPGERVA
                     IEGRGRRIAGDIVKPPFKRNP"
     gene            complement(3618..4085)
                     /locus_tag="VEV43_03510"
     CDS             complement(3618..4085)
                     /locus_tag="VEV43_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22618.1"
                     /translation="MTITGLHAHDERAGSHDDAPAALPHEAVAFAHNSERQFAKLLDF
                     YEIEWVYEPTTFDIEWDREGNATQRFSPDFYLPAFDLYIEITTLNQKLVTKKNRKVRR
                     LTALYPDVKCKILYQKDYLNLLVKYGLEEPSQGALLTPVEEAGATPPLFGVAG"
     gene            complement(4082..4582)
                     /locus_tag="VEV43_03515"
     CDS             complement(4082..4582)
                     /locus_tag="VEV43_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYP22619.1"
                     /translation="MEKELPTEGYRGPQTCKIVGITYRQLDYWTRTGLVAPSIQPATG
                     SGTQRLYSFNDLLQLKMIKSLIDAGASLQSVRKAIEYVRTHVDGDWSKVTIVASGNDV
                     YACTSDAEILDLLRNGQGVLGAMVAVDTIREQLTGTLRELRPASDPVEYGSDAPQEQE
                     RRAKEG"
     gene            complement(4683..5162)
                     /locus_tag="VEV43_03520"
     CDS             complement(4683..5162)
                     /locus_tag="VEV43_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HYP22620.1"
                     /translation="MVELSLVGVRVELPSNQPIVLLKEADGDRYLPIWIGAVEATAIA
                     FALQGIQTPRPMTHDLMRDLLQDAGVAVERIVINELVDQTFFALIRMANNGETKEVSS
                     RPSDAIALAVRINAPIYAAEEVLDQAGIELKDDEETEVEKFREFLDQVSPEDFAAEG"
     gene            complement(5191..5796)
                     /gene="hpt"
                     /locus_tag="VEV43_03525"
     CDS             complement(5191..5796)
                     /gene="hpt"
                     /locus_tag="VEV43_03525"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HYP22621.1"
                     /translation="MVTDGVVRAQPVEVLIDAARLRARIEELGAELADAYATAERPPV
                     FVGVLKGSVLFLADLVRALPFDVEVDFMSISSYSAGGAQSGVVRIVKDLDSDVHDRDV
                     VIVEDIVDTGLTLNYLRRNLGARSPRSLRAVTLLDKSVRRIVPVPLEHRGFDIPDVFV
                     VGYGLDFQALYRNAGDLLSVPDLPRLANDPRLLVGGLWPGS"
     gene            complement(5790..6908)
                     /locus_tag="VEV43_03530"
     CDS             complement(5790..6908)
                     /locus_tag="VEV43_03530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYP22622.1"
                     /translation="MSLAALVAGVLVPAAPARTAPLPPHPAVTCAACLVTTGDGVLWG
                     REVDSRRANASTTKMLTALIVVRDAELDEVVSTSRNAAATGGGGFDLRPGDRFTVGDL
                     LHALLLSSSNDAAVALAEHVAGTEQAFVARMNRLAGSFGARGSHFVTSHGLDVPGHYS
                     TARDLARIALALLRRPALAEIVALPSATIAGSSGTHRLVNTNPLLETYEGAIGVKTGY
                     TAGAGDVLVAAAERRGRRLIAVAMGSSVASEDAAALLDYGFLVLGRTVLVEEGEPVGE
                     LVFDPSGATAVVAGRTLRGIPDPARVEVSLVPARVTPPLAEGETVGTMVVTLPTGEVV
                     ARGPAVAGGELDDHGGGGAGFFGSLRGAAASVVGARSW"
     gene            complement(6978..7700)
                     /locus_tag="VEV43_03535"
     CDS             complement(6978..7700)
                     /locus_tag="VEV43_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYP22623.1"
                     /translation="MPLSRSRDYLSIGEVLDSVRADFPDVSISKIRFLEAEGLIAPER
                     TPAGYRKFFEPDVARLRYILSLQRDHFLPLRVIKERLAEADRTGSYPESEAPTAPAAN
                     GRGRAAAPEPEPALVEVTVQMTRSELRDAAGLTDHQMGELEDFGVIGKRDSDVYDAND
                     LVLARTSGRFFAYGVEPRHLRMYRQFADREATFFEQIVTPALMRKDPEGADEAHRSVK
                     ELLALSRQMREAALRSSLGRLV"
     gene            complement(7711..8187)
                     /locus_tag="VEV43_03540"
     CDS             complement(7711..8187)
                     /locus_tag="VEV43_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYP22624.1"
                     /translation="MFCTNCGHKNPDGANFCSSCGAPLAEAGSDSTITFAAPELEGEL
                     DAEIHVSPEELEGGRGVLIVKRGPNAGSKFFLDTDVTQVGRHPDSDIFLDDITVSRRH
                     AELRRKPGEFSLADVGSLNGTYVNRERVEDARLRSGDEIQIGKFKLVFLTGSPGGG"
     gene            complement(8228..8605)
                     /gene="gcvH"
                     /locus_tag="VEV43_03545"
     CDS             complement(8228..8605)
                     /gene="gcvH"
                     /locus_tag="VEV43_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HYP22625.1"
                     /translation="MTDVPADRQYSKDHEWAQEQSGRIVVGITHYAQDQLGDVVFVSL
                     PEPGTEVRAEEPLGDLESTKSVSDVYSPVTGKILEKNGEVEQNPELVNQDPYGAGWLV
                     SIEPSEPPSGLMSADEYRALIEE"
     gene            complement(8690..>10645)
                     /locus_tag="VEV43_03550"
     CDS             complement(8690..>10645)
                     /locus_tag="VEV43_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HYP22626.1"
                     /translation="PYDFSSELFPLMLDKGLPLFGFVTDTYWTDVGNTEAYIQANYDA
                     LSGKVAVDLAGFELQPGVWVGDDVEINPTARVEGPCLIGDNVRIDAGAKIGPLTVIAD
                     NAIVCDDASITGSVLLERAQAGPYSLLRGAILGRGASVHRGGNLEEGVVLGDDVTVGA
                     GAFIKARVKVYPSRTVEAGAIVTQSVVKERRATRSLFGSRGVSGPVNLGVTPQVAVRL
                     GMAYGTTLKRGSVVVTGRDASRAARTMKRALIAGLNSTGVNCHDLELMPMPVTRFAVR
                     SEQASGGISVRTSPRDPESVEIRLFDADGADLAPGDQRKIERIFFREDYRRAGALQLG
                     ELEFPPRTLERYSSGVMGALDTDLVRNSGLKVVVDYAYGAASVIGPSVLGRLGCDVLG
                     LHGFIDEHRPVLVERDIDRLLDGLAAQVRSSGSHAGVLLEPGGEAAHLVDDQGRVVSG
                     TQALLAFLRHEARRADGGTLVVPVSCPAVSEEIVAAEGATLQRASTGLAGLMARAARP
                     EVVFVGDCSGALMWPALLPAPDGLMTFCKTLELMAAEGRTLSAIVDDLPETNVVTVDV
                     PTPWDLKGAVMRHVASQAAARGGKLVLLDGVKVVEPDSWALVIPYPDEPLCRVWAEAP
                     TRAEAEALAGRYAALVAEVVDGGPDEA"
BASE COUNT         1563 a         3740 c         3756 g         1586 t
ORIGIN      
        1 cgtcgcgacg aactcgtgca tgcacgtgcc ctggaacgcg gtcgggaagg agtcggagat
       61 gagctcgcgg aggtagttcg cgttcagcac cgcgcgctcc gccacccgct tcaggccctc
      121 gggcccgagg caccgcaggt acgcgtaggc gcgcacgttg atgccgaagt tcccgtgcca
      181 cgagtggatg cgtccgatcg agtccggccg gtcgtcgtcc cagcgccatc tgcccgcgcc
      241 ctcgtcgtgc acgagcacgg gggccggcag gaagcgcgcg agcctctcgg agaccgcgag
      301 ggggccggag cccggccctc ccccaccgtg cggcgtcgcg aacgtcttgt gcaggttgag
      361 gtggacgatg tcgaagccca tgtcgcccgg gcgcacgacg ccgacgatcg agttgaagtt
      421 cgcgccgtcg taatagagga gcccgccgac ctcgtgcaac gcggcggcga tctcctggat
      481 gtcccgctcg aacaggccga gcgtgttcgg gttcgtcagc atcagccctg cgacgtcgtc
      541 gtcgagcacc tcgcgcagag cgtcgaggtc gacgaggccg cggtcgtccg acggcacctc
      601 ctgcgcggtg aagccggcga ggctcacgct cgcggggttc gtcccgtggg ccgagtccgg
      661 gatgatcacg cggtggcgcg tctcgcctcg cgacgcgtgg aacgcgcgca tgatcagcag
      721 cccggtgagc tcgccctggg ctccggccgc gggctggagc gtcagcgcag ccattccggt
      781 cgcctcgcac agcgcgtctt gcaggatccc cagcagctcg agcgccccct gcgcgagctc
      841 gtcgtcggcg gagggatgga gcgcgcggaa gccctcgagc gcggccgcgt cctccgcgag
      901 cttcgggttg tacttcatcg tgcaggagcc gagcgggtac gcgccgacgt cgatcgccca
      961 gttccgttgc gagagacgag tgtagtgcga cacgaggtcg cgctcgctga cctccggcag
     1021 cacgggcggc gcgctacgca ggaaagacga cggaatcagg tcctccaccg cgccgtcgcc
     1081 cggatccggc aacgaccagg agcggcgacc ggggcgcgat cgctcgaaga tcggcgtcga
     1141 gccgtcctcg cccgcgcggg tccccgctga ttgctcgagc gtgctcatcc ctcggccagc
     1201 tccttctcga tcgcctcggc gagcccgtcg atctcggctt cggtgcgcct ctcggtcacg
     1261 gcgacgatca gcgacttctc catgccctcg tagtgcgtgc cgagcgaggg tcctgtcagg
     1321 tagccgcggc gcgcgaggcg cttcgcgaga tcgcgcccgt cgatcggagt ctcgagcacg
     1381 agctccttga agcgcggccc gtcgaacgcg agacggcagc ccggcagcgc ggcgagtcgt
     1441 tccgccgcaa gcgcggtccg cttcacgcag agctcgccca agcggcgcag accttcgggg
     1501 ccgagccacg acaggtagat cgtcgcggcg atcgccatca gagtctggtt cgtgcacacg
     1561 ttcgacgtcg ccttggcgcg gcggatgtgc tgctcgcgcg cctgaagtgt gaggacgtat
     1621 gcccggcgac cgtcggcgtc gacggtctcg ccggcgatcc ggccgggtac ctggcggacg
     1681 agcgagagct tcgtcgcgaa cagcccgacg taggggccgc cgaacgagag cgagttgccg
     1741 agcgcctggc cctcccccac ggcgacgtcg gcgccctggg cccccggcga ctcgaggagg
     1801 cccatcgcgg tcgggtccgc gacggacacg aggagcgccc cggcgtcgtg ggcggcggtc
     1861 gccgcggctc ccgtgtcctc gagcagcccg aagtagctcg gggactggac gacgaccgca
     1921 gcggcaccgg agaggtcgtg cgcggcccag tcgatcgccc cggcgcccgt cttcgggacg
     1981 gagcggatgt ccaaccctag acctgaggtg taggatcgga gcacgccgac gtagtggggg
     2041 tggacggtct cggcgacgaa cacgacttcc cgtttggtcg cccgcacggc gaggttcacg
     2101 ccctcgacca gcgcgttggc cccgtcgtag agggaggcgt tcgcgacctc catcccgtag
     2161 agctcgcaga ccatcgtctg gaactcgaac agcgcctgga gcacgccctg ggagagctcc
     2221 ggctgatacg gcgtgtagct ggtcgcgaac tcgccccggc tcgccagcgc ccgcacggcc
     2281 gcggggacgt agtggtcgta cgcccctccc ccggcgaagc agacgaggcc ggcgcctgtc
     2341 gacttcgcag ccagctcgga catgtggccg gcgaggtcga tctcgctcag cgactcgggc
     2401 acgtcgagca ggcgcgcgag ccggacggct tcgggaacgg actcgaagag ctcgtcgagg
     2461 gagccgagac ctacggcctc gagcatctcc cggtcgtctt ccggcgtgtg aggaatgaag
     2521 tccactaggt gatttgccta cttgtcgaca agtctacgga ttccgcttga acggcggctt
     2581 cacgatatcc cccgcgatcc tgcggccgcg cccctctatc gcgaccctct cccccggctc
     2641 cggccgagtg gccgccgggc cgagcgcgag cgcgatcccg gtgccgagcg tcggcgagaa
     2701 gttcccgctg gtgagctcgc cgacccgctc gtcgccggtg aagaccccgt agccctggcg
     2761 cggcacgccc ttgtccgtgc agacgatccc gaagaggcgc cgcgccgggc ccccgccctc
     2821 cttgatcgca agcagggcct cgcgccccct gaacggcgtg tcccacgcga gagcccagcc
     2881 gagccccgcc tcgagcgggt tgatctcgcg gctgagctcg tggccgtaga gcgcgtagcc
     2941 catctcgagc cgcagcgtgt cccgggcccc gaggccgacg ggcgccgccc ccgcgtcgag
     3001 cagcgccgtc cacgcccgct cggccacctc gaagggcgcg tagatctcga accctcgctc
     3061 tccggtgtag cccgtgcgtg cgacgaggcc ggccgagccg aagacgtcga ggctgcacca
     3121 gtggtggagc ttcagcgtca gggcctcggc ccccggccac accttctcga agacggtgaa
     3181 cgagtccggg ccctgaaggg ccaggatcgc gaagcggtcc cactcgtcga ccggctcccc
     3241 tcctgcctcg cggatgcact cggcgaccgc gtgcacgttc gctgcgttcg ggacgaccag
     3301 ccattcgtcg gatgcgatcc ggtagacgaa gacgtcgtcc acgcaaccgc cgtcgtcgac
     3361 gagcgcgagc gcgtacgtcg cccggcccgg ctcgagcccg acgacgtccg ccgtcagcgc
     3421 gcgctggagc gcgtcgccgc cacccttcac gcgcagcttg ccgaggtgcg tcacgtcgaa
     3481 cagccccgcc gacgtccgca cggagttgtg ctccgcgatc acgccctcgt actgcaaggg
     3541 catctgccat ccggcgaact cggtcaggcg cgcgccgagg gcctggtgcg ccgcgagcag
     3601 cggcgtcgcg cggtcggtca tcccgcgacg ccgaacaagg gtggggtcgc gccggcctcc
     3661 tcgaccggcg tgagtagcgc gccctgcgag ggctcctcga gcccgtactt cacgagcagg
     3721 ttcaggtagt ccttctggta caggatcttg cacttgacgt cggggtagag cgcggtgagc
     3781 ctccgcacct tgcggttctt cttcgtcacg agcttctggt tcagcgtcgt gatctcgatg
     3841 tagaggtcga aggccggcag gtagaagtcc ggcgagaagc gttgcgtcgc gttgccttcg
     3901 cggtcccact cgatgtcgaa cgtggtgggc tcgtagaccc actcgatctc gtagaagtcg
     3961 agcagcttcg cgaactgacg ctcgctgttg tgtgcgaagg cgacggcttc gtggggaagt
     4021 gcagcggggg cgtcgtcgtg cgacccggcc cgctcatcgt gggcgtggag gcccgtgatg
     4081 gtcacccctc cttggctcgg cgctcctgct cctgcggtgc atccgaaccg tactccaccg
     4141 ggtcggaggc gggacggagc tcgcgcaaag tcccggtgag ctgctcgcgg atcgtgtcga
     4201 ccgcgaccat cgcgccgagc acgccctggc cgttgcgcag gaggtcgagg atttcggcgt
     4261 cggacgtgca cgcgtagacg tcgttgccgc tcgcgacgat cgtgaccttc gaccagtcac
     4321 cgtcgacgtg cgtgcgcacg tactcgatcg ccttgcggac gctctgcagg ctggcgccgg
     4381 cgtcgatgag cgacttgatc atcttcagct ggaggaggtc gttgaacgag tacaggcgct
     4441 gggtccccga cccggtcgcc ggctggatcg acggcgccac gaggcccgtg cgggtccagt
     4501 agtcgagctg gcggtaggtg atcccgacga tcttgcacgt ctgggggccg cggtacccct
     4561 cggtgggaag ttccttctcc atcacgcctc cccggcggtt gagtcacagg cccgtagtta
     4621 cgggtctgca agagtacggc gggcgcgcgc gcctgtcaac gatcgaaggg tgaaggcgcg
     4681 cgttagcctt ctgcggcgaa gtcttcgggg ctgacctggt cgaggaactc ccggaacttc
     4741 tcgacctcgg tctcctcgtc gtccttgagc tcgatcccag cttggtcgag gacctcctcg
     4801 gccgcgtaga tcggggcgtt gatcctgacc gccagggcga tcgcgtcgct cggccgggac
     4861 gagacctctt tggtctcgcc gttgttcgcc atccggatga gcgcgaagaa agtctggtcg
     4921 acgagctcgt tgatcacgat ccgctcgacc gcgaccccgg cgtcctggag caggtcgcgc
     4981 atgaggtcgt gggtcatcgg ccgcggcgtc tggatgccct ggagcgcgaa cgcgatcgca
     5041 gtcgcctcga cggcgcctat ccagatcggg aggtaacggt cgccgtcggc ttccttcaac
     5101 aagacgatcg gttgattcga tgggagctca acccggaccc ccacgagcga cagctcgacc
     5161 atgccccgca ggctaacacc gcaccagcca ctacgagccc ggccagagac cccccacgag
     5221 gagccgcgga tcgttcgcga gacggggcag atcgggcacc gagagcaggt cgccggcgtt
     5281 gcggtacagc gcctggaagt cgaggccgta gccgacgacg aacacgtcgg ggatgtcgaa
     5341 gccgcggtgc tcgagcggca ccgggacgat gcggcgcacg gacttgtcga gcaacgtcac
     5401 cgcgcgcagc gagcgcggcg aacgcgcgcc taggttgcgg cgcaggtagt tcagcgtgag
     5461 gcccgtgtcg acgatgtcct cgacgatcac gacgtcgcgg tcgtgcacgt cggagtcgag
     5521 gtctttcacg atgcggacga cgccggactg cgctcccccg gcggagtagg acgagatcga
     5581 catgaagtcg acctcgacgt cgaacgggag agcgcgcacg aggtccgcga ggaacagcac
     5641 cgagcccttg aggacgccga cgaacacggg cggccgctcc gccgtcgcgt acgcgtccgc
     5701 gagctcggcc ccaagctcct cgatccgcgc ccgcagacgc gccgcgtcga tcagcacctc
     5761 gaccggctgc gcgcgtacga cgccgtcggt caccacgacc tcgctcctac gaccgacgcc
     5821 gcggcccccc ggagcgatcc gaagaacccc gcgcccccgc ctccgtggtc gtcgagctcg
     5881 cctcccgcga ccgcggggcc gcgagcgacc acctcgccgg tcggcaacgt caccaccatg
     5941 gtccccaccg tctcaccctc ggcaaggggc ggcgtcaccc gggccgggac gagcgacacc
     6001 tcgacgcggg ccgggtcggg gatcccccgc agggtcctgc cagccaccac ggcggtggcg
     6061 cccgacgggt cgaacaccag ctctccgacg ggctcccctt cctcgacgag gaccgtccgg
     6121 ccgaggacca ggaagccgta gtcgaggagc gcggccgcat cctccgaggc gaccgacgac
     6181 cccatggcga ccgcgatcag acgacggccg cggcgctcgg ccgcggcgac caagacgtca
     6241 cctgcacctg cggtgtagcc ggtcttcacg ccgatcgcac cctcgtaagt ctccagtaga
     6301 ggattagtgt tcaccagccg gtgggtcccg gaggagcccg cgatcgtggc cgacgggagc
     6361 gcgacgatct ccgcgagggc cggacgccgc agaagggcga gcgcgatccg ggcgaggtcc
     6421 cgcgccgtcg agtagtggcc gggcacgtcg agcccgtgcg aggtcacgaa gtgcgagccc
     6481 cgggccccga acgacccggc gaggcggttc atccgcgcca cgaaggcctg ctcggtgccg
     6541 gcgacgtgct cggcgagcgc aacggcggcg tcgttggacg acgacagcag cagagcgtgc
     6601 aggagatccc cgaccgtgaa cctgtcgcca ggccggagat cgaagccccc tcccccggtc
     6661 gcggccgcgt tcctcgaggt gctcacgacc tcgtcgagct ccgcgtcgcg cacgacgatc
     6721 agggccgtga gcatcttcgt cgtgctcgcg ttcgcccggc gcgagtcgac ctcccgcccc
     6781 catagcaccc cgtcccccgt ggtcacgaga caggctgcgc acgtgacggc ggggtgcggc
     6841 gggagcggag ccgtgcgcgc gggggccgcg ggaacgagca ccccggcaac gagggccgct
     6901 agagacacgg ctacaaatcg atttgtcgac aaggcgacga gactactagg agacgatccg
     6961 ggtgggccgg aggggtgcta gacgagccgg ccgaggctgg agcggagggc ggcctcgcgc
     7021 atctgccgcg acagcgccag cagctccttc accgaacggt gcgcctcgtc ggccccctcg
     7081 gggtccttgc gcatcagcgc gggcgtcacg atctgctcga agaaggtggc ctcgcggtcg
     7141 gcgaactggc ggtacatgcg caggtgccgg ggctcgacgc cgtaggcgaa gaagcgcccg
     7201 gaggtccggg ccagcacgag gtcgttggcg tcgtagacgt cggagtcccg cttgccgatc
     7261 acgccgaagt cctcgagctc gcccatctgg tggtcggtca ggccggcggc gtcacgcagc
     7321 tcgctgcgcg tcatctggac cgtgacctcg acgagcgccg gctcgggctc gggcgcagcg
     7381 gccctgcccc gcccgttcgc ggcgggagcg gtgggggcct cggactcggg gtaggagccg
     7441 gtccggtcgg cctcggccag gcgctccttg atcaccctga gcggcaggaa gtggtcccgc
     7501 tggagcgaca ggatgtagcg caggcgggcg acgtccggct cgaagaactt gcggtagccg
     7561 gcgggcgtgc gctcgggcgc gatcaggccc tcggcctcga ggaaacggat cttcgagatg
     7621 ctgacgtcgg ggaagtcggc gcggacggag tcgaggacct cgccgatgct caggtagtca
     7681 cgagagcggg acagcggcac ttgagacccc ctatcccccg cccggcgagc cggtcaggaa
     7741 gacgagcttg aacttgccga tctggatctc gtcgccggag cggagccgtg cgtcctcgac
     7801 gcgctcgcgg ttcacgtacg tgccgttgag gctgcccacg tcggcgagcg agaactcgcc
     7861 cggcttccgc cgcagctcgg catgacgccg cgagaccgtg atgtcgtcga ggaagatgtc
     7921 gctgtcgggg tgacgtccga cctgggtgac gtccgtgtcg aggaagaact tgctgccggc
     7981 gttcggcccc cgcttgacga tcaggacccc ccggccccct tcgagctcct ccggggagac
     8041 gtggatctcg gcgtcgagct cgccctcgag ctcgggggcg gcgaacgtga tcgtcgagtc
     8101 ggagcccgcc tcggcaagag gggctccgca ggacgagcag aagttggcgc cgtcggggtt
     8161 cttgtggccg cagttcgtgc agaacatgcg gctgacccta taggcgagct ttaaccttcg
     8221 tcaacactca ctcttcgatc agcgcgcggt actcgtcggc gctcatgaga ccggaggggg
     8281 gctcgctcgg ctcgatcgac acgagccacc ccgcgccgta gggatcctgg ttgacgagct
     8341 cggggttctg ctcgacctcg ccgttcttct cgaggatctt gccggtcacc gggctgtaga
     8401 cgtccgagac cgacttcgtc gactcgaggt cgccgagggg ctcctcggct ctcacctcgg
     8461 tccccggctc cggaaggctc acgaacacga cgtcgccgag ctggtcctgc gcgtagtgcg
     8521 tgatcccgac gacgatgcgg ccgctctgct cctgcgccca ctcgtggtcc ttcgagtact
     8581 gccggtcggc gggtacgtcg gtcaccgggg tctctccttt cggcggggcg tatcgacttg
     8641 tcgacttgtg tccaagtctc cgagaagact tgtcgtgtct cggaccgggt caggcctcgt
     8701 cgggcccgcc gtcgacgact tccgcgacga gcgcagcgta ccggcccgcg agggcctccg
     8761 cctcggcgcg cgtcggcgcc tccgcccaca cgcggcagag cggctcgtcg ggataaggga
     8821 tgaccagcgc ccacgaatcg ggctcgacga ccttgacccc gtcgaggagc actagcttgc
     8881 cgccgcgcgc ggccgcctgc gacgcgacgt gccgcatgac cgcgcccttg aggtcccacg
     8941 gcgtcggcac gtcgacggtc accacgttcg tctcgggcag gtcgtcgacg atcgccgaca
     9001 gcgtccgtcc ctcggccgcc atcagctcga gggtcttgca gaacgtcatg agcccgtcgg
     9061 gggccgggag cagcgcgggc cacatcagcg cgccgctgca gtcgccgacg aacacgacct
     9121 ccggccgcgc ggctctcgcc atgagccccg ccagaccggt cgaggcgcgc tggagggtcg
     9181 cgccttcggc cgcgacgatc tcctcggaca ccgcggggca ggacaccggg acgacgagcg
     9241 tgccgccgtc cgcgcgcctc gcctcgtggc ggaggaacgc gagcagtgcc tgggtccccg
     9301 agaccacgcg gccctggtcg tcgacgaggt gtgcggcctc gccgccgggc tcgagcagga
     9361 cgccggcgtg ggagccggag ctccgcacct gtgcggccag cccgtcgagg agccggtcga
     9421 tgtcccgctc gacgaggacc gggcggtgct cgtcgatgaa accgtggagc ccgaggacgt
     9481 cgcagccgag ccggccgagg accgaggggc cgatcacgga cgccgcgccg tacgcgtagt
     9541 cgacgacgac cttgaggccg ctgtttctga cgaggtccgt gtcgagcgct cccatcaccc
     9601 cggacgagta gcgctcgagc gtgcgcggcg ggaactcgag ctcgccgagc tggagcgcgc
     9661 ccgcccgccg gtagtcctcg cggaagaaga tccgttcgat cttgcgctgg tcgcccggcg
     9721 cgaggtccgc gccgtcggca tcgaacagcc ggatctcgac gctctcgggg tcgcgcggcg
     9781 acgtgcgcac gctgatccct ccggacgcct gctcgctgcg gacggcgaag cgcgtgacgg
     9841 gcatcggcat cagctcgagg tcgtggcagt tcaccccggt cgagttcagc ccggcgatca
     9901 gcgctcgctt catcgtccgt gccgcgcggc tcgcgtcgcg gccggtgacg acgaccgagc
     9961 cccgtttcag cgtcgtgccg taggccatgc cgagcctgac cgctacctgg ggggtgacgc
    10021 cgaggttcac cgggccggag acgccgcggg agccgaagag gctgcgcgtc gcccggcgct
    10081 ccttgaccac gctctgcgtc acgatcgccc cggcctcgac cgttcggctc gggtaaacct
    10141 tcactcgagc cttgatgaag gcgcccgcgc cgaccgtgac gtcgtcgccg aggaccacgc
    10201 cttcctcgag gtttccacct cggtgaacgg acgctcccct gccgaggatc gcgccccgca
    10261 gcagtgagta aggcccggcc tgggccctct cgaggaggac ggagccggtg atcgacgcgt
    10321 cgtcgcagac gatcgcgttg tcggcgatga cggtgagcgg cccgatcttg gcccccgcgt
    10381 cgatccggac gttgtcgccg atcaggcagg ggccctcgac ccgggcggtc gggttgatct
    10441 cgacgtcgtc gcccacccat acgccgggct gcagctcgaa cccggccagg tcgacggcca
    10501 ccttgccgga cagcgcgtcg tagttcgcct ggatgtacgc ctcggtgttg ccgacgtccg
    10561 tccagtaggt gtcggtgacg aagccgaaca gcggcaggcc cttgtcgagc atcaacggga
    10621 acagctcgct ggagaagtcg tacgg
//
LOCUS       DASQDO010000116         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_929444, whole genome shotgun sequence.
ACCESSION   DASQDO010000116 DASQDO010000000
VERSION     DASQDO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929444"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VEV43_03555"
     CDS             complement(<1..405)
                     /locus_tag="VEV43_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22627.1"
                     /translation="MWDFPHGSLAGREAAAYLVSEAAGWNVVPPTVLREDAPLGPGSL
                     QLFVEHDPERHYFVLMEERAEDLVAFAAFDVVINNADRKSGHVIEDAEGKLWAVDHGV
                     TFHLEDKLRTVIWGYAGNPLDAVTIAKLETLGA"
     gene            complement(545..1063)
                     /locus_tag="VEV43_03560"
     CDS             complement(545..1063)
                     /locus_tag="VEV43_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HYP22628.1"
                     /translation="MQFDVTPDVFTADFTGQPGARTFYLQARAPSGTHSFLLEKQQVA
                     ILAEKLQEMLLMIDENDTIKSAAPQRDPGLGLEEPVDPEWRIGTIGLLYDEGRDVVVV
                     VVQEVAEDDEEEEDDESGYRFFLRRDQVRSLILHALAAVGEGRPLCQLCGLPMDPAGH
                     VCPASNGHRPSG"
     gene            complement(1063..>1661)
                     /locus_tag="VEV43_03565"
     CDS             complement(1063..>1661)
                     /locus_tag="VEV43_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HYP22629.1"
                     /translation="LVRHGVTSHTGHKLSGWLPDVHLTDEGREQADAAAELLATMNLE
                     AVYSSPIDRTRETAAAIAARHGLPVRTRRTIGEVEYGKWTNRSFKVLARQKLWGLVQR
                     WPSSVRFPDGETLREVQARALAEVEKIAEAHPNGMVCIVSHADVIKLVLAHYLGIHID
                     LFQRIVIAPASVSGVRLTTDGPRILSMNVSPRLLTRPV"
BASE COUNT          250 a          596 c          547 g          268 t
ORIGIN      
        1 cgcacccagc gtctcgagct tcgcgatcgt gaccgcgtcc agaggattcc ccgcataacc
       61 ccagatcacc gtccgcagct tgtcctccag gtggaacgtc acgccgtggt cgaccgccca
      121 cagcttcccc tccgcgtcct cgatcacgtg gcccgacttg cggtccgcgt tgttgatcac
      181 gacgtcgaac gcggcgaacg cgacgaggtc ctcggcgcgc tcctccatca gcacgaagta
      241 gtgacgctcg gggtcgtgct cgacgaagag ctgcagcgaa ccggggccga gcggggcgtc
      301 ctcgcgcagc acggtcggcg ggacgacgtt ccagcccgcg gcctcgctga cgaggtacgc
      361 ggcggcctcg cggccggcca ggctcccgtg cgggaagtcc cacaacgggc gctcgccgcg
      421 ccgcggcttg tagacggcga gcgtctccgg tgcgacgcgc gtcaggaacg tgtagttcga
      481 ctagtacggc aacagaccga ggacctcgat ctcgccggtc cgcagcacct cgagcgtctg
      541 ctcgtcatcc actcggtcta tggccattgg acgcgggaca tacgtgcccg gcggggtcca
      601 tcggcaaccc acacagctgg cagagcggcc gcccctcccc caccgcggcc agcgcgtgga
      661 ggatcaacga ccgcacctgg tccctcctca agaagaaccg gtagccggac tcgtcgtctt
      721 cctcctcctc gtcgtcttcg gcgacctcct gcaccacgac gaccacgacg tcgcggccct
      781 cgtcgtagag cagcccgatc gtgccgatgc gccattcggg atcgacgggc tcctcgaggc
      841 ctaggccggg atctcgctgg ggggccgccg acttgatcgt gtcgttctcg tcgatcatca
      901 gcagcatctc ctggagcttc tccgccagga tcgccacctg ctgcttctcg aggaggaacg
      961 agtgcgtgcc cgacggcgcg cgcgcctgca agtagaaggt ccgcgcgccc ggctggccgg
     1021 tgaagtccgc ggtgaagacg tcgggtgtga cgtcgaactg catcagaccg gcctcgtgag
     1081 caaacgcggc gagacgttca tcgacaggat ccgcggcccg tcggtcgtga gccgcacgcc
     1141 gctgaccgac gccggcgcga tcacgatcct ctggaagagg tcgatgtgta tgccgaggta
     1201 gtgcgcaagc actagcttga tgacgtcggc gtggctcacg atgcacacca tcccgttcgg
     1261 gtgcgcctcg gcgatcttct cgacctcggc gagagcgcgg gcctggacct cgcgcagggt
     1321 ctcgccgtcg gggaaccgga ccgacgacgg ccatcgctgc acgagccccc acagcttctg
     1381 ccgcgccagg accttgaacg agcggttcgt ccacttgccg tactcgacct cgccgatggt
     1441 ccgccgcgtg cgcaccggaa ggccgtggcg tgcggcgatc gcggccgcgg tctcgcgcgt
     1501 cctgtcgatc gggctcgagt acacggcttc gaggttcatc gtcgcgagga gctccgccgc
     1561 ggcgtccgcc tgttcgcggc cctcgtcggt caggtggacg tccggcaacc accccgacag
     1621 cttgtgcccg gtgtgcgagg tgacgccgtg cctgacgagc c
//
LOCUS       DASQDO010000117         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_72929, whole genome shotgun sequence.
ACCESSION   DASQDO010000117 DASQDO010000000
VERSION     DASQDO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72929"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VEV43_03570"
     CDS             <1..271
                     /locus_tag="VEV43_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HYP22630.1"
                     /translation="QLREKGFAVVGTDAAAETPYDALDYAGRSAFVVGNEAWGIAEEH
                     RSALTDVVSIPMPGDAESLNAGIAGAILLFEAVRQRRGRLGGTAD"
     gene            293..964
                     /locus_tag="VEV43_03575"
     CDS             293..964
                     /locus_tag="VEV43_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYP22631.1"
                     /translation="MTDNARFADMISKIAHELRSPLTSVKGFSATLVKRWDRFTDEQR
                     LQFIETIHHDAERMGRIVSEVLDLARLEAGRLELQVQPVSLHEVASKAAANHSDLGGA
                     DRVRIDVPEDAQVFADPERLGHALSNLVENAIKFSDEGPIVVASNDGEITISDEGVGI
                     PEDQIAEVFSGPAPHAQKSGPSGTGLGLYLTRRLVEAHGGSISVESAVGKGSRFTIKL
                     PKTPA"
     gene            961..2019
                     /gene="pheS"
                     /locus_tag="VEV43_03580"
     CDS             961..2019
                     /gene="pheS"
                     /locus_tag="VEV43_03580"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006363086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYP22632.1"
                     /translation="MSELDELRAQFEEARSQGLAAIAEAGDMAAVQEARVRTLGRKTP
                     LSRARSSLGRVPDEARKELGRLANEVQQSLEGALAAKTAEFEEAERAERWERERVDVT
                     LPGEHVPTGSVHPLTKTIWEIVDVFVGLGYAVADGPEVELSTLNFDALNAPPEHPSRS
                     PADTFYVEGTDERVCLRPQTSPAQIRTMQSQPPPVYIVVPGRCYRRDEEDATHLAGFA
                     QIEGLAVDEGITVADMKGTLELFARELFGKDLATRLRGHFFPFTEPSCEMDVECFVCR
                     GSGCRMCKGEGWIELLGCGMVDPALFEYVGYDPERYSGFAFGLGVERAAALSHGVSDI
                     RAMYENDVRFLSQFEGVS"
     gene            2016..4403
                     /gene="pheT"
                     /locus_tag="VEV43_03585"
     CDS             2016..4403
                     /gene="pheT"
                     /locus_tag="VEV43_03585"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYP22633.1"
                     /translation="MRVSLAWLREFVDVDVPLNALKEMLDLSGTKVDKVHRPGEGISG
                     VVVAQVQEIDAHPNADNLSMVTVSTHEGAQRVVCGAKNFAVGDKVALARVGATLPEMT
                     ITERKIRGEVSAGMLCSASELGVSKDHAGLLILPEDAELGQDVVRTLGLDDAIFELEI
                     TPNRPDCMSVVGVAREVAALTGNELRLPSAEVSVEDDLQAPVTVRIEDAEGCPRFVAR
                     YLTGVKVGPSPQWMASRLVAAGVRPVSNVVDVTNYVMMELGHPLHAYDAAKVADGLIV
                     VRRAAPGESLTTLDGVDRTLVPDDLVIAGRDGVLGIAGVMGGATSEVSDETTDVILEC
                     AYFDHVTISYTSRRYGLRTEASARFERGADPEAPPFAAARAARLMAELAGARVSAAVV
                     DEYPAPVERPSITLRPHRTSKVLGEDVPADRQARHLRSIGIEVEEAEGTFQTRVPSFR
                     PDLTREIDLVEEVIRLYGIDRVASTLPPGRAGRLDEVQRFDRALRRTLASLGLREAWT
                     DSFLGPKELDDLGLAADHPARSMVRLANPLVDERPGLRTTLLPGLLRAVARNVAHRAD
                     GVALFEIARVYEPTGDELPLEAQVLAAIFSGRRRPQGWTAAGASWDFFSAKGVLEAAL
                     SSLRLPAPELSPVEGMPFHPTRGASVSIAGTVAGAFGELHPDVCERFDVPEGAVAFEL
                     SLAPVVSALPERVKASDLPKFPPLLIDLAVVVDEEVPARTVAEIVRETGAPDVTSARL
                     FDLYRGDQVPAGKKSLAFALEIRAEDRTLTDEEAMQVRDRIVTALAERAGAELRA"
     gene            4414..5331
                     /gene="argF"
                     /locus_tag="VEV43_03590"
     CDS             4414..5331
                     /gene="argF"
                     /locus_tag="VEV43_03590"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYP22634.1"
                     /translation="MEERDFLSIDDVSPEELRYLVDSAAALKRKPDALDDTLKGRRVA
                     LIFEKPSTRTRVSFEAAVTSLGGSAIVLRGDELQLGRGETIEDTGRVLSRYVSAIVVR
                     TFGQDRLERLAHAASVPVVNALSDFSHPCQCLADLQTIAEKKGSLDGLTLTYVGDGNN
                     VAHSLMFGGTKMGMHVRIGCPEGYEPFPQVVDRCREIGESTGGSVTVTHSPEEAARDA
                     DVLYTDVWASMGQESEAESRVGVFEPYRLDSALVDVSDRDSIVMHCLPAHRGEEISAD
                     VIDGPRSVVWDQAENRLHAQKALLAWLLG"
     gene            5394..>5916
                     /locus_tag="VEV43_03595"
     CDS             5394..>5916
                     /locus_tag="VEV43_03595"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HYP22635.1"
                     /translation="MVFAHRTPEGIAAVRLVETEAYRGPLDPGSHGYRGVTPRTEVMY
                     GPPAHLYVYFTYGMHWCANVVCGRAGECEAVLLRAGEPLVGADLMRLRRGEVGDKLLA
                     SGPARLAQALGIARSHNGATLLRGGDFWCAEDDLTNDLRDGEVSQTTRIGLAEGRGDD
                     IPWRFVVPGHPHAS"
BASE COUNT          885 a         2069 c         2098 g          864 t
ORIGIN      
        1 gcagctgcgc gagaaggggt tcgcggtcgt cggcaccgac gcggccgcgg agacccccta
       61 cgacgcgctc gactacgccg gccgcagcgc gttcgtcgtc ggcaacgagg cgtgggggat
      121 cgccgaggag caccgctccg ccctcaccga cgtcgtctcg atccccatgc cgggggacgc
      181 ggagtccctc aacgccggca tcgccggtgc gatcctgctc ttcgaggcgg tccgccaacg
      241 acggggtcgc ctcggcggga ccgcggacta gacggatatc ctcggcggct acatgacgga
      301 caacgcacgc ttcgccgaca tgatctcgaa gatcgcgcac gagctgcggt ctccgttgac
      361 gtccgtgaag gggttctcgg cgacgctcgt caagcgctgg gaccgcttca ccgacgagca
      421 gcgcctgcag ttcatcgaga cgatccatca cgacgccgag cgcatgggcc ggatcgtctc
      481 cgaggtcctc gacctcgccc ggctcgaagc gggacggctc gagcttcagg tccagcccgt
      541 atcgctgcac gaggtcgcgt cgaaggccgc tgccaaccac tccgacctcg ggggggcgga
      601 cagggtccgt atcgacgtcc ccgaggacgc acaggtcttc gcggaccccg aacgtctcgg
      661 tcacgcgctg tcgaacctcg tcgagaacgc cataaagttc tccgacgagg ggcccatcgt
      721 ggtcgcctcg aacgacggcg agatcacgat ctcggacgag ggcgtcggga tccccgaaga
      781 ccagatcgcc gaggtcttct ccggcccggc cccccacgcg cagaagtccg gtccctcggg
      841 caccgggctc ggtctctacc tgacgcgccg gctcgtcgag gcgcacggcg gctcgatctc
      901 cgtcgagagc gcggtcggga agggctcgag gttcacgatc aagctcccga agacacccgc
      961 atgagcgagc tggacgagct gcgcgcgcag ttcgaggagg cgcgttccca gggcctcgcc
     1021 gcgatcgcgg aggccggcga catggcggcg gtccaggagg cgcgggtgcg gacgctcggc
     1081 cgcaagaccc cgctgtcgcg agcgcgctcg tcgctcggcc gcgtgcccga cgaagcacgc
     1141 aaggaactgg gccggctcgc gaacgaggtc cagcagagcc tcgagggcgc gctcgccgcc
     1201 aagaccgccg agttcgagga ggccgagcgc gccgaacgct gggagcgcga gcgcgtcgac
     1261 gtcacgctcc ccggcgagca cgtcccgacc ggatcggtgc acccgctgac caagacgatc
     1321 tgggagatcg tcgacgtgtt cgtcgggctc ggctacgcgg tcgcggacgg gcccgaggtc
     1381 gagctctcta ccctcaactt cgacgcgctc aacgcgccgc cggagcaccc gtcgcgctcg
     1441 ccggccgaca cgttctacgt cgaagggacg gacgagaggg tctgcctgcg accgcagacg
     1501 tcgcccgcgc agatccgcac gatgcagtcc cagcccccgc cggtctacat cgtcgtcccc
     1561 ggccgttgct accgcaggga cgaggaggac gcgacgcacc tggccgggtt cgcgcagatc
     1621 gagggccttg cggtcgacga ggggatcacc gtcgccgaca tgaaggggac gctcgagctg
     1681 ttcgcacgcg agctcttcgg caaggacctc gccacgcgtc tgcgcggcca cttcttcccg
     1741 ttcaccgagc cgtcttgcga gatggacgtc gagtgcttcg tctgccgagg cagcggctgc
     1801 cgcatgtgca agggcgaggg atggatcgag ctcctcgggt gcgggatggt cgacccggcc
     1861 ctgttcgagt acgtcggcta cgaccccgag cgctactcgg ggttcgcgtt cggactcggc
     1921 gtcgagcggg ccgcagccct ctcgcacggc gtctcggaca tccgtgcgat gtacgagaac
     1981 gacgtgcggt tcctgagcca gttcgagggt gtgtcgtgag ggtcagcctc gcgtggctgc
     2041 gtgagttcgt cgacgtagac gtcccgctga acgcgctcaa ggagatgctc gacctgtcgg
     2101 ggaccaaggt ggacaaggtc caccggcccg gtgagggcat atcgggcgtc gtcgtcgcgc
     2161 aggtccagga gatcgacgcg cacccgaacg cggacaacct gagcatggtc acggtctcga
     2221 cgcacgaggg ggcccagcgc gtggtctgcg gcgcgaagaa cttcgccgtc ggcgacaagg
     2281 tcgcgctcgc tcgcgtgggc gccaccttgc cggagatgac gatcaccgag cgcaagatcc
     2341 gcggggaggt cagcgcgggc atgctgtgct ccgcgtccga gctcggcgtg tcgaaggacc
     2401 acgcaggtct cctgatcctg ccggaggacg cggagctcgg ccaggacgtc gtccggactc
     2461 tcgggctcga cgacgcgatc ttcgagctgg aaatcacccc taaccgtccc gactgcatga
     2521 gcgtcgtcgg agtcgcgcgt gaggttgcgg cgctgaccgg gaacgagctc aggttgccgt
     2581 ccgcggaggt ctccgtcgag gacgacctgc aagcgccggt gacggttcgg atcgaggacg
     2641 ccgagggctg tccccgtttc gtggcccggt acctcacggg cgtgaaggtg gggccgtcgc
     2701 cgcagtggat ggcgtcgcgt ctggtcgcgg caggcgtgcg cccggtctcg aacgtcgtcg
     2761 acgtgaccaa ctacgtgatg atggagctgg gccaccccct gcacgcctac gacgccgcca
     2821 aggtcgcgga cgggctcatc gtcgtgcgcc gcgccgcacc gggcgaatcg ctgaccaccc
     2881 tcgacggcgt ggaccgcacg ctcgttcccg acgacctcgt gatcgccggg cgcgacggcg
     2941 tcctcggcat cgccggcgtc atgggaggcg cgacctcgga ggtttcggac gagacgaccg
     3001 acgtgatcct cgagtgcgcg tacttcgacc acgtcacgat ctcgtacacg agccgccggt
     3061 acggcctgcg caccgaggcc tcggcccgct tcgagcgcgg cgccgacccc gaggctccgc
     3121 cgttcgcggc cgcgcgggca gcgcgcttga tggcagagct cgccggcgcg cgcgtatcgg
     3181 ccgcagtggt ggacgagtac ccggcccccg tggagcgccc gtcgatcacc cttcgccccc
     3241 accggacgtc gaaggtgctc ggcgaggacg tccccgccga ccgccaggcc cgtcacctcc
     3301 gctcgatcgg catcgaggtc gaggaagccg aggggacgtt ccagactcgg gtcccgtcgt
     3361 tccgtccgga cctcacgcgt gagatcgacc tcgtcgagga ggtcatccgc ctctacggca
     3421 tcgaccgcgt cgcgtcgacg ctgccgcccg gacgggcggg ccggctcgac gaggtccagc
     3481 ggttcgaccg cgcgctgcga cggacgctcg cgtcgctcgg gctccgcgaa gcgtggaccg
     3541 actcgttcct cggccccaag gagctcgacg acctcgggct cgccgcagac caccccgccc
     3601 gcagcatggt gaggctcgcc aacccgctcg tcgacgagag gcccggcctc cgcacgacgt
     3661 tgcttcccgg gttgctgcga gccgtagcgc gcaacgtcgc acacagagcc gacggcgtag
     3721 ccctgttcga gatcgcacgt gtgtacgagc ccaccggcga cgagctgccg ctggaggcgc
     3781 aggtgctcgc cgcgatcttc agcgggcgga ggcgaccgca gggctggacc gccgccggcg
     3841 cgagctggga cttcttctcc gcgaaggggg tgctcgaggc cgcgttgagc tcgctgcgcc
     3901 ttcccgcgcc cgagctctca ccggtcgaag ggatgccctt tcacccgacc cgcggagcct
     3961 ccgtctcgat cgcggggacc gttgccggag cgttcggcga gctccatccc gacgtctgcg
     4021 agcggttcga cgtccccgaa ggcgccgtag cgttcgagct gtcgctcgcg ccggtcgtgt
     4081 cggcgctgcc ggaacgtgtg aaggccagcg acctgccgaa gttcccgccg ctgctcatcg
     4141 acctcgcggt cgtcgtggac gaggaggtcc cggcgcgcac ggtcgccgag atcgttcgcg
     4201 agaccggtgc ccccgacgtc acctccgccc ggctcttcga cctctatcgc ggcgaccagg
     4261 tcccggccgg caagaagagc ctcgcgttcg ccctcgagat cagggccgag gaccgtacgt
     4321 tgaccgacga ggaggcgatg caggtgcgtg accggatcgt cacggctctc gccgagcggg
     4381 ccggcgcgga gctgcgcgcc tagaggaggg tttgtggagg agcgcgactt cctgtcgatc
     4441 gacgacgtga gccccgagga gctgagatac ctcgtcgact ccgcggctgc gctgaaacgc
     4501 aagcccgacg ccctcgacga caccctgaag ggtcgccggg tcgcgctgat cttcgagaag
     4561 ccgagcacgc gcacgcgcgt ctcgttcgag gccgccgtga cgtcgctggg cggctccgcg
     4621 atcgtgttgc gcggcgacga gctccagctc ggacggggcg agacgatcga ggacaccggg
     4681 cgagtcctgt cgcgctacgt ctcggcgatc gtcgtccgga cgttcggcca ggaccgcctc
     4741 gaacggctcg cgcatgcggc atccgtcccg gtcgtcaacg cgctcagcga cttctcccac
     4801 ccgtgccagt gcctcgccga cctgcagacg atcgcggaga agaagggatc gctcgacggg
     4861 ctcacgctga cgtacgtcgg cgacggcaac aacgtcgcgc actcgttgat gttcggcggc
     4921 acgaagatgg gcatgcacgt ccggatcgga tgccccgagg gatacgagcc gttcccgcag
     4981 gtcgtcgacc ggtgccgcga gatcggcgag tcgaccggag gtagcgtcac cgtcacgcac
     5041 tcgccggagg aggctgcgcg cgacgcagac gtcctctaca ccgacgtgtg ggcgagcatg
     5101 ggacaggagt ccgaggcgga atcccgggtc ggcgtgttcg agccctatcg gctcgactcg
     5161 gccctcgtcg acgtctccga tcgcgattcg atcgtgatgc actgcctccc ggcgcaccgc
     5221 ggtgaggaga tatcggccga cgtgatcgac ggcccccgct cggtcgtctg ggaccaagcc
     5281 gagaaccgcc tgcacgcgca gaaggcgctg ctcgcctggc tgttgggctg agacggcttc
     5341 cacgcgcgtt ctacgcgcgc gacgcgctga cggtcgcccg cgagctgatc ggtgtggtgt
     5401 tcgcgcaccg gacgcccgag gggatcgcgg ccgtgcgact cgtcgagacc gaggcgtacc
     5461 gcggccccct cgaccccggc tcgcacggat accgcggcgt gacaccgcgc accgaggtca
     5521 tgtacgggcc gccggcgcat ctgtacgtgt acttcacgta cggcatgcac tggtgcgcga
     5581 acgtcgtttg cggccgcgcc ggagagtgtg aggcggtgct gttgcgagcg ggggagccgc
     5641 tcgtcggagc ggacctgatg cgcctccgcc gcggcgaggt gggggacaag ctgctcgcgt
     5701 cgggcccggc ccgtctcgcg caagccttgg gcatagcgag gtcccacaac ggcgcgacgc
     5761 tgctgcgcgg aggcgacttc tggtgcgcgg aagacgacct gacgaacgac ctacgcgacg
     5821 gcgaggtctc gcagacgacg aggatcgggc tggccgaggg ccggggcgac gacatcccgt
     5881 ggcgcttcgt ggtcccgggc cacccgcacg cctccc
//
LOCUS       DASQDO010000118         5567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_684911, whole genome shotgun sequence.
ACCESSION   DASQDO010000118 DASQDO010000000
VERSION     DASQDO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684911"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /gene="thiD"
                     /locus_tag="VEV43_03600"
     CDS             <1..666
                     /gene="thiD"
                     /locus_tag="VEV43_03600"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HYP22636.1"
                     /translation="AQNTVAVTGVHEVDPRFVVEQVEAVATDIGVDAAKTGMLASAAL
                     VDAVAKAVEAFDIPRLVVDPVAVSKHGDRLLAEDAVDAVRTRLLPLAEVVTPNLHEAA
                     TLAGIEVTTMAQMKDAARAIHALGPRTVLVKGGHVDGPDSVDLFYDGEDFTELRGPRF
                     DTKDTHGTGCALSAAIAARLAHGDSTIEALHHAKRFISGAIERSLRIGNGYGPVNPGW
                     ELL"
     gene            complement(641..1480)
                     /locus_tag="VEV43_03605"
     CDS             complement(641..1480)
                     /locus_tag="VEV43_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22637.1"
                     /translation="MTEAIPEHRPGFPAPIRIDGYFAWTWRLFRLCFWRLATVFVAGV
                     VVASVVHLGIVILMLEVFDTGQTVQALAVSFAAQVTLSAVAGTLLAAVAAPVFVGEVA
                     GRRTGADEGWRRLRPNLGYVVVSALYVAMPLLMLVLFLGQIIQVLLLPAVLGPPVLVH
                     AIVWEGRDFRDGATRAKNLLAGSWGRVISALLVLAIGPALLQIVTLTAFGELLPGLDG
                     DQLADSVWATLAIAVTTAPVWLFTTAAGTVAYLDLRARFEELDGADLAAEAEAARGAP
                     TPG"
     gene            complement(1486..1677)
                     /locus_tag="VEV43_03610"
     CDS             complement(1486..1677)
                     /locus_tag="VEV43_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HYP22638.1"
                     /translation="MGKDARQTLGEIEETREDLARKVDELVDRAKVEAGEIGKKLAVG
                     AAALAGLLIVGLIAKRRVR"
     gene            complement(1686..2114)
                     /locus_tag="VEV43_03615"
     CDS             complement(1686..2114)
                     /locus_tag="VEV43_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYP22639.1"
                     /translation="MAERPRAQDLYKGNGAGADKSAGQLMKEVTEDLSTLIRKEVELA
                     KQEVGQSIATKVKGVVVILIAGVIGLFALIFALFAIRDGFDELLWTWAADLATAGVLL
                     LLAAGAGLIAKKKLTAPVSAEMTKQSLKDDAEMVKSLGKR"
     gene            complement(2125..2997)
                     /locus_tag="VEV43_03620"
     CDS             complement(2125..2997)
                     /locus_tag="VEV43_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYP22640.1"
                     /translation="MPDFSKPINQAKLRIEKARARYGLVDIVVRTFKRHSEDDGGFYA
                     AALTYYVFFSMFPLLIFATALLGFATQIYPDLEQDLLTEGLQSFPLVGSIIEPDVLRA
                     IRRGAGGLAVLSIGLALYSGSGAVVALGHALNKIHRVENEGNFFVKRLDALKWLGLFA
                     LMVLGSITVTGWAQWVPGWGPTIISLLVGIALNLAIFATAFKFLPRMRRTWEDVLPGA
                     VIAAGVFELLKYVGTLYVSRGAEARSATFGAFATAAGLLVASYLLAQITLLTAEVNAV
                     LAERRLTRQSLLKG"
     gene            complement(3042..3230)
                     /gene="rpmB"
                     /locus_tag="VEV43_03625"
     CDS             complement(3042..3230)
                     /gene="rpmB"
                     /locus_tag="VEV43_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYP22641.1"
                     /translation="MASVCDICGKTPGFGNNISHSHRRTRRRWNPNVQKMRVLVGGAP
                     KRVAVCTKCLKANKVTRG"
     gene            3280..5487
                     /gene="recG"
                     /locus_tag="VEV43_03630"
     CDS             3280..5487
                     /gene="recG"
                     /locus_tag="VEV43_03630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYP22642.1"
                     /translation="MSQTVRARPKGLELYATGRAAAPLPVTGKRAEALEKLGIGSVQD
                     LLQHYPRYHVDRTQLRTIADLRRLAAEEHDGEVQVHATVKKMNRPFRTRSGKVMIKGQ
                     IADETGAIDVTWFNQEWVVRALQPGTVAFFYGKLGLYRGRLQMSAPRFERVRTGAEPF
                     NVGRIVPIYPATADLTSDQLRKLMWDALAKHGTILDPLPESLFRGLGLMSRSDAVRLI
                     HFPDSRDDVIGARRRIVFDEVFTLQLGLVYRKRKLERTVQGIAHEPPGASSLAETFVA
                     ELPFDLTGAQRRACGEVAADMAKAHPMHRLVEGEVGSGKTVVALYACLVAIQGGYQAA
                     FMAPTEVLAEQHHLTVGELLDRAFGEAERANLFAGVSRRPVVRLLTGSTPAAQRRKIL
                     DEVARGEVDLLLGTHALIQESVAFARLGVAVVDEQHRFGVHQRKTLREKGAEGEPDIL
                     VMTATPIPRTLAVTIYGDLDVSILDELPKGRKPIETRLAALAGERDEAYDLIRREVAS
                     GRQAFVVYALRDESDKTELRSAKAEAKRLAEEVFPDLEVGLVHGDMKSADKEASMTAF
                     RDGKTDVLVATTVIEVGVDVPNATVMLIEDADRFGLAQLHQLRGRIGRGGHASYCVLA
                     TDLDLTKADPAIALVRERLEAVASTGDGFRLAEVDLRHRGEGQLFGARQSGMPQLKMT
                     RVLQHQDVIQKARAMAVAILDEDPELRAYEHAELAREMHARFPDQALDVVHSG"
BASE COUNT          902 a         1949 c         1920 g          796 t
ORIGIN      
        1 gcacagaaca ccgtcgcggt gacgggcgtg cacgaggttg accctcggtt cgtcgtcgag
       61 caggtcgagg ccgtcgccac cgacataggg gtcgacgcgg cgaagaccgg catgctcgcg
      121 tcggccgcgc tcgtcgacgc cgtcgcgaag gccgtcgagg cgttcgacat ccctcggctg
      181 gtggtcgacc ccgtggcggt gagcaagcac ggtgacaggc tgctcgccga ggacgccgtc
      241 gacgcggtgc gaacgcggct gctgccgctc gccgaagtgg tgacgccgaa cctccacgag
      301 gccgcgacgc tggccggtat cgaggtgacg acgatggctc agatgaagga cgcggcgcgc
      361 gccatccacg cgctcggccc ccgcaccgtc ctcgtcaagg gcgggcacgt cgacggcccc
      421 gactcggtcg atctgttcta cgacggcgaa gacttcaccg agctgcgggg gccgcgcttc
      481 gacacgaagg acacgcacgg gaccggttgt gctctctcgg cggcgatagc cgcgcgccta
      541 gcgcacggcg attcgacgat cgaagcgctc caccatgcga agcgcttcat ctccggcgcg
      601 atcgagcgct cgctacggat cgggaacggc tacggcccgg tcaacccggg gtgggagctc
      661 ctctagccgc ctcggcttcg gcggcgaggt cggcgccgtc cagctcctcg aaccgagccc
      721 gcaggtcgag gtacgcgacc gtcccggccg cggtcgtgaa cagccacacc ggggccgtcg
      781 tcaccgcgat cgccagcgtc gcccagaccg agtcggctag ctggtcgccg tccagtccgg
      841 gcaggagctc gccgaacgcc gtgagcgtca cgatctgcag gagcgccggc ccgatcgcca
      901 ggacgaggag cgccgagatg acgcggcccc agctccccgc gaggaggttc ttcgcgcgcg
      961 tcgcaccgtc ccggaagtcg cgcccttccc agacgatcgc gtgcacgagg acggggggac
     1021 cgaggaccgc cgggagcagc agcacctgga tgatctggcc gaggaacagc acgagcatca
     1081 gcaacggcat cgcgacgtag agggccgaca cgacgacgta gccgaggttc ggccgcagcc
     1141 ggcgccaccc ttcgtccgct ccggtgcgac ggccggccac ctcgccgacg aagaccggtg
     1201 cggcaacggc ggcgagcagc gtccccgcga ccgcggacaa ggtgacctgc gccgcgaagc
     1261 tgacggctag cgcttgcacc gtctgaccgg tgtcgaagac ctcgagcatg aggatcacga
     1321 tcccgaggtg gaccaccgac gcgacgacga cgcccgcgac gaagaccgtc gcgagcctcc
     1381 agaagcacaa ccggaacagg cgccacgtcc acgcgaagta gccgtcgatc cggatgggcg
     1441 cggggaagcc cggccggtgc tcgggtatgg cttcggtcac gcggtctagc ggacccggcg
     1501 cttggctatc agaccgacga tgagcagccc cgccagggca gctgctccga cggcgagctt
     1561 ctttccgatc tcgcccgcct cgaccttcgc gcggtcgacg agctcgtcga ccttgcgcgc
     1621 gaggtcctcc cgcgtctcct cgatctctcc cagcgtctgg cgggcgtcct tgcccatgcc
     1681 gcctcctatc tcttccccaa ggacttcacc atctcggcgt cgtccttgag ggactgcttg
     1741 gtcatctcgg ccgagaccgg ggcggtcagc ttcttcttgg cgatcagccc ggcccccgcc
     1801 gccagcagga gcaacacgcc cgccgtcgcg aggtcggccg cccacgtcca caggagctcg
     1861 tcgaagccgt cgcggatggc gaacagggcg aagatcagag cgaatagccc gatgacgccc
     1921 gcgatgagga tgacgacgac acccttgacc ttcgtcgcga tcgactggcc gacctcctgc
     1981 ttggcgagct cgacctcctt gcggatcagc gtcgagaggt cctcggtgac ctccttcatc
     2041 agctgccccg cggacttgtc cgcgccggcc ccgttcccct tgtagaggtc ctgcgctctc
     2101 ggtcgttcgg ccacgatccc tcctctatcc cttgagcagc gattgtcgcg tcaaccggcg
     2161 ctcggcgagt acggcgttga cttccgcggt cagcagcgtg atctgggcga gcagatagct
     2221 ggcgaccagg aggcccgcag cagtggcgaa tgcgccgaag gtcgcgctgc gcgcctcggc
     2281 ccccctgctg acgtagagcg tcccgacgta cttgagcagc tcgaagaccc cggctgcgat
     2341 cacggctccc ggcagcacgt cctcccaggt ccggcgcatg cgtggcagga acttgaaggc
     2401 cgtggcgaag atcgcgaggt tcaaagcgat ccccacgaga aggctgatga tcgtcgggcc
     2461 ccaaccgggc acccactgag cccaccccgt cacggtgatc gagccgagca ccatcaacgc
     2521 gaacagcccg agccacttca aggcgtccag ccgcttgacg aagaagttcc cctcgttctc
     2581 gacacggtgg atcttgttca gcgcgtgtcc gagcgcgacc acggcgccgc tgcccgaata
     2641 gagcgcgagg ccgatgctca ggacggcgag gccccccgcc ccgcgccgga tggccctcag
     2701 cacgtcgggc tcgatgatgc tgccgaccag ggggaagctc tgcagcccct ccgtgagcag
     2761 gtcttgttcg aggtccgggt agatctgcgt ggcgaagccg agcagcgcag ttgcgaagat
     2821 cagcagcggg aacatcgaga agaagacgta ataggtaagg gccgcggcgt agaagccgcc
     2881 gtcgtcctcg gagtggcgct tgaaggtcct gacgacgatg tccacgaggc cgtagcgggc
     2941 gcgcgccttc tcgatgcgga gcttcgcctg gttgatcggc ttggagaagt cgggcatcgc
     3001 gcggggatac ccgtgcggct gcgcccgcta aacagtgcga gctagccgcg cgtgaccttg
     3061 ttcgccttca ggcacttcgt gcagacggcc acgcgcttcg gcgcaccacc gacgagcacg
     3121 cgcatcttct ggacgttggg gttccaacgc cggcgcgtgc gcctgtggga gtgcgagatg
     3181 ttgttcccga accccggcgt cttcccgcag atgtcacata cagaagccat aggggagcga
     3241 gggtaccaaa atccggctgc tcccccataa cctggcccca tgagccagac cgtccgggcc
     3301 cggcccaagg gcctcgagct gtacgcgacg ggccgcgcgg ccgcgccgtt gcccgtgacc
     3361 ggcaagcggg ccgaggcgct cgagaagctg gggatcgggt cggtccagga cctcctccag
     3421 cactaccccc gctaccacgt cgaccgcacg cagctgcgga cgatcgccga tctccgccgg
     3481 ctcgccgcgg aggagcatga cggcgaggtc caggtgcacg cgaccgtcaa gaagatgaac
     3541 cgcccgttcc gcactcgctc gggcaaggtg atgatcaaag gccagatcgc cgacgagacc
     3601 ggcgcgatcg acgtcacgtg gttcaaccag gagtgggtgg tccgagcgtt gcagccgggg
     3661 accgtcgcct tcttctacgg gaaactcggg ctgtaccgcg gccgtctcca gatgagcgcg
     3721 ccgcgcttcg agcgagtgag gacgggggcc gagcccttca acgtcggccg gatcgtcccg
     3781 atctatccgg cgaccgcgga cctgacgagc gaccagctcc ggaagctgat gtgggacgcg
     3841 ctcgccaagc acggcacgat cctcgacccc ctgccggagt cgttgttccg cgggctcggg
     3901 ctcatgagcc ggtccgacgc cgtgcgcctg atccacttcc cggactcgcg cgacgacgtc
     3961 atcggggctc ggcgcaggat cgtcttcgac gaggtcttca cgctgcagct cggcctcgtc
     4021 taccgcaagc gcaagctcga acggacggtg caggggatcg cccacgagcc accgggcgcg
     4081 tcgtccctcg cggagacgtt cgtcgcagag cttcccttcg acctgaccgg cgcgcagcgg
     4141 cgtgcctgcg gtgaggtcgc cgccgacatg gccaaggcgc acccgatgca ccggctcgtc
     4201 gagggagagg tcggctcggg gaagaccgtc gtcgccctgt acgcgtgcct cgtcgcgatc
     4261 caaggcggct accaggccgc gttcatggcg ccgaccgagg tcttggccga gcaacatcac
     4321 ctcacggtgg gggagctgct cgaccgggcc ttcggcgagg ccgagcgcgc gaacctcttc
     4381 gccggggtgt cccggcgtcc tgtggtccgt ctcctgacgg gctccacacc cgccgcccaa
     4441 cggcggaaga tcctcgacga ggtggcgcgc ggcgaggtcg acctcctgct cggcacgcat
     4501 gcgctcatcc aggagtccgt cgcgttcgcg cggctcggcg tcgccgtcgt cgacgagcag
     4561 caccggttcg gagtgcacca gagaaagacc ctgcgcgaga agggggccga gggtgagccc
     4621 gacatcctcg tgatgacggc gacgccgatc ccgcgcacgc tcgcggtcac gatctacggg
     4681 gacctcgacg tgtcgatcct cgacgagctg ccgaaggggc ggaagccgat cgagacgcgg
     4741 ctcgccgctc tcgccggtga gcgggacgag gcctacgacc tgatccggcg cgaggtcgcc
     4801 tcggggagac aagcgttcgt cgtctacgcg ttgcgcgacg agtccgacaa gaccgagctg
     4861 cgcagcgcga aggccgaggc gaagcgcctc gccgaggagg tgttccccga cctcgaggtc
     4921 gggctcgtcc acggcgacat gaagtcggcc gacaaggagg cgtcgatgac ggcgttccgc
     4981 gacggcaaga ccgacgtcct ggtcgcgacg accgtgatcg aggtcggcgt cgacgtgccg
     5041 aacgcgaccg tgatgctgat cgaggacgcg gatcgcttcg ggctcgcgca gctccatcag
     5101 ctgcggggcc gcatcggccg cggtggccac gcgtcctact gcgtgctcgc gaccgacctc
     5161 gacctcacga aggccgaccc ggcgatcgcg ctcgtgcggg agcggctcga ggccgtggct
     5221 tcgaccgggg acgggttccg tctggccgag gtcgacctcc ggcaccgcgg cgagggccag
     5281 ctgttcggtg cccgccagtc ggggatgccg cagctcaaga tgacccgggt gctccagcac
     5341 caggacgtga tccagaaggc ccgggcgatg gcggtggcga tcctggacga agaccccgag
     5401 ctgagggcct acgagcacgc cgagctggcc cgcgagatgc acgcccgctt cccggaccag
     5461 gccctggacg tagtccactc gggctgacgc aacccccctc ccgcgtgcgc cgcccgtggc
     5521 ggaaatcggc atgggcggcg cacgcggggc cggggggagg ctgacgc
//
LOCUS       DASQDO010000119         7310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_501434, whole genome shotgun sequence.
ACCESSION   DASQDO010000119 DASQDO010000000
VERSION     DASQDO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501434"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VEV43_03635"
     CDS             <1..657
                     /locus_tag="VEV43_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HYP22643.1"
                     /translation="ALSREESAPDLAPEPVDQLDLREWADGAKSYLARLVGGVPELEA
                     MSDRIVAQIDKVKTLTAPGSKTRIHGDYHLGQVLRGLKWMILDFEGEPVRSLEERRTK
                     HSPLKDVAGMLRSFSYAATSALFARAEPDSDEWKRLQPWADTWEALARERFLASYLRK
                     SHEGRFLAQERETLGVLLDFFEIDKALYELEYELGHRPEWVRIPATGIARVIERGEKR
                     "
     gene            654..2819
                     /gene="glgB"
                     /locus_tag="VEV43_03640"
     CDS             654..2819
                     /gene="glgB"
                     /locus_tag="VEV43_03640"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYP22644.1"
                     /translation="MTTADRAAVEQLLRGELKDPHYVLGPHPGPKKSTVVRAWRPEAQ
                     TVQCVVDGETVAKLELVDPAGLFEGSVDGELGDYQLEVGYPDGTFTLRDPYAFAPTLG
                     ELDLHLLGEGNHRRLHDYLGAHAREVDGVTGTSFAVWAPNARSVRVVGDFNSWDGRLH
                     PMRLLGGSGIWELFVPDVGPGAFYKFEILTANGDISLKTDPFAFATQAPPETASLVQQ
                     PAHRFGDDGWMDRRETTDWHSQPMSIYEVHLGSWRRDENGEPLSYREMAPQLADYCRE
                     LGFTHVELLPVAEHPFGGSWGYQVSHYFSPTARFGSPDDFRYLVDTLHQAGIGVIVDW
                     VPAHVPKDEWALARFDGTALYEHLDPRKGEHPDWGTLVFNFGRNEVRNFLISNALYWI
                     ESFHVDGLRVDAVASMLYLDYSREEGQWIPNAFGGRENLEAIEFLKQLNEVVHDEHPG
                     VLMLAEESTAWPGVSRPVHLGGLGFGFKWNMGWMHDTLSYFSKDPIHRRYHHNNLTFS
                     LMYAWTENFVLPLSHDEVVHGKGSLIGKMPGDKWRRLANLRSLLAYMWAHPGKQLLFM
                     GGELGQEREWNHDRALDWHVLDDPAHRGLHKMVADLNAAYQATPALWEQDFTPEGFQW
                     IDANDADNNVISFYRKATDGSVLVCICNLSALPRSEFRVGLPKEGRYAEVLNTDAAPY
                     GGTNTGNMGFVDAVERPWHGLDYSADVTLPPLGVVWLHA"
     gene            2804..4204
                     /locus_tag="VEV43_03645"
     CDS             2804..4204
                     /locus_tag="VEV43_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HYP22645.1"
                     /translation="MAARIAVHGHFYQPPRENPWTGTIEVQQGAAPYRDWNERVHAES
                     YRPNTRVELADGRVVNNFEKLSFNVGPTLLAWMQEADRDTYDAIVGADHGNAMAQAYH
                     HTILPLSPVRDVRTQVRWGLRDFELRFGRRSEGIWLPETAADDRTVDVLIEEGVGFTI
                     LAPGQAASDGPLDTTVPYRVEHSDGSGRFLTVMFYDGDISHAIAFDGAARSAEGLTGL
                     FEARSGEGCRLVHSATDGETFGHHQKFSDLGLAYALFVEAPKRGLEVVSYPEFLSECP
                     PERSIRLRPGGTSWSCAHGVGRWNEDCGCSTGGEEGWNQKWRGPLRRALEVIRQAADD
                     VYERAGREVFTHPWGARDRDIDVVSGAIELEEFVVRETGGDAFMARAAALLDLQRYAL
                     SMYTSCGWFFNDLAGIETVQVMLYAARTMELLEGLGASAPREEFLSILGEAQSNDPDE
                     GSGAEIFERAVGAAKP"
     gene            complement(4215..5333)
                     /locus_tag="VEV43_03650"
     CDS             complement(4215..5333)
                     /locus_tag="VEV43_03650"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22646.1"
                     /translation="MKRTLVSLAAALTMIAAPTAQAIDLGVAGPGPHFASSENIEWIT
                     NMPLAVDSAGANFVGDYMYITTSRDLTIYDISNPESPQRMGFLPLPQQPYYAEEDVDT
                     NGKILLIGTYPADLYVIDVEDKANPVILGQVGNADQHTWTCVLDCTWAYGSEGLIADL
                     RNPAEPKIVGNWGEGKPAGGSHDVTEVAPGLIVTSSSPMLYLDARKNPAKPKLLGVGA
                     GPEGQYMHGNMWPRNAKDKFLLVGSESTGPQCEDGAYFMTYDATKIKKTKTFNLIDKY
                     TVRNGNPTEGDAAADLFCGHWFDAHPTFRNGGLVAMAWYEHGTRFLKVDSKGKITEVD
                     WFLPIGGSTSATYWVTDEILYSVDYNRGLDILRYTGKP"
     gene            complement(5370..>7310)
                     /locus_tag="VEV43_03655"
     CDS             complement(5370..>7310)
                     /locus_tag="VEV43_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HYP22647.1"
                     /translation="FYAFRRSHSDMRLSTDGGRTWPRTDTPGVVDSAAIGRNPSEIVI
                     SVRGRLYFFDPPTREWVGIEVPRSGIGGLESDLGLEVDFYGHTAGTIEKYDGPVSRTV
                     GPDPDGGPVFDVPGLINPDGVVKPLPPALGPDDAKVRMKTGAKRTVDYRLKLSAKETM
                     LDVFFLLDTSDTMSGTIQGLIDSVGSIINRLRAAGLDAHFGIGVSRAYTDTAVPRPTC
                     ESETDQNCERNFIYRRLVDLSDYSNDDQLRAALASLQAEAGGRFDSQLGALWAIATGE
                     EIDDVPTGTMEPSDVPAGQDASFREGALNLIFVATDEPFATGEEGDRNNLTVSDFGRI
                     VPPDIPGYQEVADAVLAADAKVLGLAIGQTLKTDNDSVEGDTPTAFRSMTEIANLTGA
                     IAPEGGVDCDGDGVPELAAGDALVCPVRRHNVAEGENLAAAITKLVEAVRGTREIQLE
                     VVRGEDVVSEVTPSSYPAVVKQKPNVLDFEVTYECPDRLLGKNVTVQLAAVDAGGNRL
                     DSVTTAVACLEEKVDEPPLPIEPVITLGFVIPPLMPPAPPPIAANAPSAQGQAQSQSQ
                     AQAQGAMAHQEQQQPQLAYVGATLDHRQALEEELAMSDRRRSEVPQWATLGTGAVMAS
                     LAFGWLSLSRQRSIRVQRVRRR"
BASE COUNT         1086 a         2477 c         2594 g         1153 t
ORIGIN      
        1 gcgctgtcgc gcgaggagtc ggccccggac ctcgcgcccg agccggtcga ccagctcgac
       61 ctgcgggagt gggccgacgg cgccaagtcg tacctcgccc ggctcgtcgg aggcgtgccc
      121 gagctcgagg cgatgagcga ccggatcgtg gcgcagatcg acaaggtcaa gacgctgacg
      181 gccccgggtt ccaagacgcg catccacggc gactaccacc tgggtcaggt cctccgcggg
      241 ttgaagtgga tgatcctcga cttcgagggc gagccggtgc gctcgctcga ggagcggagg
      301 acgaagcact cgccgctcaa ggacgtcgcg gggatgctgc ggtcgttcag ctacgcggcg
      361 acctcggctc tgttcgcccg cgccgagccc gactcggacg aatggaagcg gctccagccg
      421 tgggcggata cgtgggaggc ccttgcacgc gagcgtttcc tggcgagcta cctgcggaag
      481 tcgcacgagg gccgtttcct ggcgcaggag cgcgagacac tcggcgtcct gctggacttc
      541 ttcgagatcg acaaggcgct ctacgagctc gagtacgagc tcggccaccg ccccgagtgg
      601 gtgcgcatcc cggctaccgg gatcgcccgc gtgatcgaac gaggtgagaa gcgatgacga
      661 cagccgatcg cgctgcggtg gagcagctgc tgcggggaga gctgaaggac ccgcactacg
      721 tcctcggccc gcacccgggg ccgaagaagt cgacggtcgt acgcgcgtgg cggcccgagg
      781 cgcagaccgt ccagtgcgtg gtcgacggcg agacggtggc gaagctcgag ctggtcgacc
      841 ccgcggggct gttcgagggc agcgtcgacg gcgagctcgg cgactaccag ctcgaggtcg
      901 gctacccgga cggcacgttc acgctccggg acccgtatgc gttcgcgccg acactgggcg
      961 agctcgacct ccacctcctc ggcgagggca atcaccgcag gctccacgac tacctcggcg
     1021 cgcacgcgcg cgaggtggac ggcgtcaccg gcacgagctt cgccgtatgg gcccccaacg
     1081 cgcgcagcgt ccgcgtggtc ggcgacttca acagctggga cggccgcctg cacccgatgc
     1141 ggttgctcgg agggtcgggc atctgggagc tgttcgtccc cgacgtcggg ccgggcgcgt
     1201 tctacaagtt cgagatcctc acggcgaacg gggacatctc gctgaagacc gacccgttcg
     1261 cgttcgcgac gcaggcgccg cccgagacgg cgagcctcgt ccagcagccg gcccaccggt
     1321 tcggcgacga cgggtggatg gaccggcgcg agaccacgga ctggcactcc cagccgatgt
     1381 cgatctacga ggtgcatctc gggtcatggc gccgcgacga gaacggcgag ccgctgtcgt
     1441 accgcgagat ggccccccag ctcgcggact actgccgcga gctcggcttc acgcacgtcg
     1501 agctgctgcc cgtcgccgag cacccgttcg gcgggtcgtg gggctatcag gtaagccact
     1561 acttctcgcc taccgcacgc ttcggctcgc ccgacgactt ccgctatctc gtggacacgc
     1621 tccaccaggc cggcatcggc gtcatcgtcg actgggtccc ggcccacgtc ccgaaggacg
     1681 agtgggcgct cgcccgcttc gacgggaccg ccctgtacga gcacctcgac ccccgcaagg
     1741 gcgagcaccc cgactggggg acgctcgtct tcaacttcgg tcgcaacgag gtccggaact
     1801 tcctgatcag taacgcgctg tactggatcg agagcttcca cgtcgacggg ctgcgcgtcg
     1861 acgccgtcgc gtcgatgctg tacctcgact acagccgcga ggagggccag tggatcccga
     1921 acgccttcgg gggccgcgag aacctcgagg cgatcgagtt cctcaagcag ctcaacgaag
     1981 tcgtccacga cgagcacccc ggcgtcctga tgctcgcgga ggagtcgacc gcgtggccgg
     2041 gcgtaagccg tcccgtgcac ctcggcgggc tcggcttcgg cttcaagtgg aacatgggct
     2101 ggatgcacga cacgctgtcg tacttctcga aggacccgat ccaccggcgc taccaccaca
     2161 acaacctgac gttcagcctc atgtacgcgt ggaccgagaa cttcgtgctg ccgctgagcc
     2221 acgacgaggt cgtccacggc aagggctctc tgatcggcaa gatgcccggc gacaagtggc
     2281 ggcgtctcgc gaacctccgg tccctgctcg cgtacatgtg ggcgcacccc ggcaagcagc
     2341 tgctgttcat gggcggcgag ctcggtcagg agcgcgagtg gaaccacgac cgggcgctcg
     2401 actggcacgt gctcgacgac cccgcgcacc gcggcctgca caagatggtc gccgacctca
     2461 acgcggccta tcaggcgacg ccggctctgt gggagcagga cttcacgccc gagggcttcc
     2521 agtggatcga cgcgaacgac gccgacaaca acgtgatctc gttctaccgg aaggcgaccg
     2581 acggctcggt cctcgtctgc atctgcaacc tctcggcgct accgcgcagt gagttccgcg
     2641 tcggtctccc gaaagaaggg cgctacgcgg aggtcctcaa caccgacgcg gccccctacg
     2701 gagggacgaa cacggggaac atgggattcg tcgacgccgt cgagcggccg tggcacgggc
     2761 tcgactactc cgccgacgtc acgctgccgc ctctcggagt ggtgtggctg cacgcatagc
     2821 cgtccacggc cacttctacc aacccccgcg ggagaacccg tggacgggaa ccatcgaggt
     2881 ccagcagggg gcggctccgt accgggactg gaacgagcgc gtccacgccg agtcctaccg
     2941 gccgaacacg cgcgtcgagc tcgccgacgg gcgcgtcgtc aacaacttcg agaagctgtc
     3001 gttcaacgtc ggcccgacgc tgctcgcgtg gatgcaggaa gcggaccgcg acacctacga
     3061 cgcgatcgtc ggagccgatc acggcaacgc gatggcgcag gcgtaccacc acacgatcct
     3121 gccgctgtcg cccgtgcgcg acgtacgcac gcaggtccgc tgggggctgc gtgacttcga
     3181 gctccgcttc ggccgccggt cggaggggat ctggctcccc gagacggccg ccgacgatcg
     3241 cacggtcgac gtcctgatcg aggagggcgt gggcttcacg atcctggcgc cgggccaggc
     3301 cgcctccgac ggccctctcg acacgaccgt cccctaccgc gtcgagcact ccgacgggtc
     3361 gggccgcttc ctcacggtca tgttctacga cggcgacatc agccacgcga tcgcgttcga
     3421 cggagccgct cgttcggccg agggcctgac cggcctgttc gaggcacggt ccggcgaagg
     3481 gtgccggctc gtccattcgg cgaccgacgg cgagaccttc ggccaccacc agaagttctc
     3541 ggacctcggc ctcgcctacg cgctgttcgt cgaggcgccg aagcgcgggc tcgaggtcgt
     3601 ctcgtatccg gagttcctct ccgaatgccc gccggagcgg tcgatccggc tccggcccgg
     3661 cggcacttcg tggtcgtgcg cgcacggcgt cgggcgatgg aacgaggact gcggctgctc
     3721 gaccgggggc gaggaaggtt ggaaccagaa gtggcgcggc ccgctccgcc gcgcgctcga
     3781 agtgatccgc caggccgcgg acgacgtgta cgagcgggcc gggcgcgagg tcttcacgca
     3841 cccgtggggg gcccgggacc gcgacataga cgtcgtgtcc ggggcgatcg agctcgagga
     3901 gttcgtcgtg cgcgagacgg gcggcgacgc gttcatggcg cgggccgctg ccttgctgga
     3961 cctgcagcgc tacgcgctgt cgatgtacac gagctgcggc tggttcttca acgacctggc
     4021 cggcatcgag accgtgcagg tgatgctcta cgcggcgcgg acgatggagc tgctggaggg
     4081 gctgggcgcg agcgctccgc gcgaggagtt cctgtcgatc ctgggcgaag cgcagagcaa
     4141 cgacccggac gagggctcgg gggccgagat cttcgagcgg gccgtggggg ccgctaagcc
     4201 ctgaggggct gcggctacgg cttgcccgtg tacctcagga tgtccagccc ccggttgtag
     4261 tcgaccgagt acaggatctc gtccgtcacc cagtaggtgg ccgaggtcga gcccccgatc
     4321 ggcaggaacc agtcgacctc ggtgatcttg cccttcgagt cgaccttcaa aaagcgcgtg
     4381 ccgtgctcgt accaggccat cgccacgagc ccgccgttcc tgaacgtcgg gtgcgcgtcg
     4441 aaccagtgcc cgcagaagag gtcggcggct gcgtcaccct cggtcgggtt cccgttccgg
     4501 accgtgtact tgtcgatcag gttgaacgtc ttggtcttct tgatcttcgt cgcgtcgtag
     4561 gtcatgaagt aggcgccgtc ctcgcactga ggcccggtgc tctcgctgcc gacgagcagg
     4621 aacttgtcct tggcgttgcg cggccacatg ttcccgtgca tgtactggcc ctccggccct
     4681 gcgccgaccc cgagcagctt cggcttcgcg gggttcttgc gggcgtcgag gtacagcatc
     4741 ggcgagctcg acgtcacgat caggcccggc gccacctcgg tgacgtcgtg gctgccgcct
     4801 gcaggcttgc cctcgcccca gttgccgacg atcttcggct cggcggggtt gcggaggtca
     4861 gcgatgaggc cctcggagcc gtacgcccac gtgcagtcga gcacgcacgt ccacgtgtgc
     4921 tggtcggcgt tgccgacctg accgaggatg accgggttgg ccttgtcctc gacgtcgatg
     4981 acgtagaggt cggccgggta cgtcccgatg agcaggatct tgccgttggt gtcgacgtcc
     5041 tcctccgcgt agtacggctg ctgcgggagc gggaggaagc ccatgcgctg cggtgactcg
     5101 gggttcgaga tgtcgtagat cgtgaggtcg cgcgacgtcg tgatgtacat gtagtcgccg
     5161 acgaagttcg cgccggcgct gtcgaccgcg agcggcatgt tggtgatcca ctcgatgttc
     5221 tcggacgagg cgaagtgggg gccggggccg gcgacaccga ggtcgatggc ctgggcggtg
     5281 ggggccgcga tcatcgtgag cgcggcggca agggagacga gagttctctt catggggttg
     5341 gggttcgccg gggccgtccc ggctcctgct cagcggcggc ggacgcgctg gacgcggatg
     5401 ctgcgctggc ggctcagcga gagccacccg aaggcgagcg aggccatgac cgcaccggtc
     5461 ccgagggtcg cccactgggg cacctccgaa cgccggcggt cgctcatcgc gagctcctcc
     5521 tcgagcgcct ggcggtggtc gagggtcgcg ccgacgtagg cgagctgggg ctgctgctgc
     5581 tcctggtgcg ccatcgctcc ctgggcctgg gcctggctct gcgactgcgc ctggccctgg
     5641 gcgctcgggg cgttcgccgc gatcggcggg ggggccgggg gcattagcgg cgggatgacg
     5701 aagccgagcg tgatcacggg ctcgatcggc agcgggggct cgtcgacctt ctcctcgagg
     5761 cacgccaccg cggtcgtcac ggagtcgagc cggttcccgc cggcgtcgac cgcggcgagc
     5821 tggacggtca cgttcttgcc caggaggcgg tcggggcact cgtaggtgac ctcgaagtcg
     5881 aggacgttgg gcttctgctt gacgacggcc ggataggacg agggcgtgac ctcgctcacg
     5941 acgtcctcgc cgcggacgac ctcgagctgg atctccctcg tcccgcgcac ggcctcgacg
     6001 agcttcgtga tcgccgccgc aaggttctcg ccctcggcga cgttgtgccg ccgcaccggg
     6061 cagacgagag cgtccccggc cgccagctcg gggaccccgt ccccgtcgca atcgacgccg
     6121 ccttcgggag cgatcgcacc ggtgaggttg gcgatctccg tcatgctgcg aaacgccgtc
     6181 ggcgtgtccc cctcgaccga gtcgttgtcg gtcttcaggg tctgcccgat cgcgaggccg
     6241 aggaccttcg cgtcggccgc gagcacggcg tcggcgacct cttgatagcc cgggatgtcg
     6301 ggggggacga tccggccgaa gtcggagacc gtcaggttgt tacggtcgcc ctcctcgccc
     6361 gtggcgaacg gctcgtcggt agcgacgaag atcaggttca gggcgccttc acggaacgac
     6421 gcgtcctgcc cggccgggac gtcgctcggc tccatggtcc ccgtgggcac gtcgtcgatc
     6481 tcctcccccg tggcgatcgc ccacagggcg ccgagctgcg agtcgaaacg gccgccggcc
     6541 tcggcctgca ggctcgcgag agccgcacgc agctggtcgt cgttcgagta gtccgagagg
     6601 tcgaccaggc gcctgtagat gaagttgcgc tcgcagttct ggtcggtctc ggactcgcac
     6661 gtcgggcgcg ggaccgccgt gtcggtgtag gcgcgcgaga cgccgatccc gaagtgcgcg
     6721 tcgaggcccg cggcccgcag ccggttgatg atcgacccga cgctgtcgat cagtccctgg
     6781 atcgtcccgc tcatcgtgtc ggacgtgtcg agcaggaaga agacgtcgag catcgtctcc
     6841 ttggccgaga gcttcagccg gtagtcgacg gtcctcttcg caccggtctt catcctgacc
     6901 ttcgcgtcgt ccgggcccag cgcggggggc aggggcttca cgaccccgtc ggggttgatc
     6961 aggccgggga cgtcgaacac cgggccgccg tcggggtcgg gcccgacggt gcgggagacc
     7021 ggtccgtcgt acttctcgat cgtcccggcc gtgtggccgt agaagtcgac ctcgagcccg
     7081 aggtcggatt cgagcccgcc gatgcctgac cgcggcacct cgatcccgac ccactccctc
     7141 gtgggcgggt cgaagaagta gagtcggccc cgaacggaga tcacgatctc gctcgggttg
     7201 cggccgatgg cggccgagtc gacgacgccg ggagtgtcgg tgcgcggcca ggtgcggccg
     7261 ccgtcagtcg agaggcgcat gtccgaatgg ctccgccgga aggcgtagaa
//
LOCUS       DASQDO010000120         6061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_195901, whole genome shotgun sequence.
ACCESSION   DASQDO010000120 DASQDO010000000
VERSION     DASQDO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195901"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="wecB"
                     /locus_tag="VEV43_03660"
     CDS             complement(<1..918)
                     /gene="wecB"
                     /locus_tag="VEV43_03660"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HYP22648.1"
                     /translation="MTKVMLVFGARPNFMKIAPIFREMRERGGFEPVLVHTGQHFDEA
                     MSDQFLRVLELPRPEVHLEVGSGTESWQTAQIITRLEPVLAELRPDAGVVVGDVTSTL
                     AASLAMSFARVPLAHVEAGLRSRDWSMPEERNRIVVDRISQFLLTPSEDADDNLRAEG
                     IERAKIHRIGNVMIDSLDWILPRLDAQRVLADYDLGEGDAFGLVTLHRAGNVDDDAVL
                     KGLVGALADVAERLPLLFPVHPRTKKRLDALGLSFDATGVRSLPPLSYSDFTALMSRA
                     ALVLTDSGGIQEEAVVLGVPCLTLRDNTER"
     gene            complement(915..1925)
                     /locus_tag="VEV43_03665"
     CDS             complement(915..1925)
                     /locus_tag="VEV43_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HYP22649.1"
                     /translation="MSRVWVDIANSPHATFFKPVVDVLRERGHQVILTAWDRAQTADL
                     ARRYWPEVEVVGAGSDRSVVQKSLRIWKRARGLAAAVRDARPDVALGHNSYSQVIAAR
                     LLLRTRAVTMMDYEHQPANHLAFRLAHDVVVPAHFPEDAARKMGARRKLLRYDGFKEE
                     ITLAVFRPEAGFRSSLGVTDDEVLVASRPPPDGALYHRHSNDLFARAIETVAAQGATV
                     LLSPRTVEQGRSYSTIEGVRVLEHAVSGLDLLWASDLVVGAGGTMTREAAILGTPSYT
                     LFSGKPAAVDGALIESGRLVRVSDAAAVAAIPVVRKPRVEWTPPEHRVTDVVTLLEKT
                     FS"
     gene            complement(1922..3061)
                     /locus_tag="VEV43_03670"
     CDS             complement(1922..3061)
                     /locus_tag="VEV43_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22650.1"
                     /translation="MTDDEALAISVALLERWRGRDGRTYDPYDGLETRWVSSPRRLGR
                     VARLALVQVHKRSPVNLRPLFRIAPSRNAYGEGLFASARLTLWQLLGEPVHRRIAEEG
                     LAWLESARVGEGWGYPFDVQTKTFFYPKTDPNIVSTAFAANAFLDAAEATGDERWLDG
                     AVRAARMICRDFWVERGGRFWFAYLPGEPKLIHNANLLGARLCTRSASLSGDGSLADA
                     ASAAVETTLRYQRDDGSFLYGEDPDVRWVDGHHTGFVVECLHDVRATFPSVVAPLTRA
                     ARYYAEHLFGPAGEPLPAPGRPFPLDAIAGAQGIQTFAKLGDLALAERIAAWMRGMRM
                     RNGSFSYQRGRLHRKRVPYARWSDAPMALALTALVAQRRSVGAAA"
     gene            complement(3058..5271)
                     /locus_tag="VEV43_03675"
     CDS             complement(3058..5271)
                     /locus_tag="VEV43_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HYP22651.1"
                     /translation="MTTTPRRDDDEQATAQPVRRDDDEQATAQPVRRVVLSPKGAKVE
                     VELGHAPALGPGEVLIQTAFSVVSPGTERSKLELSRKTLVGKARSRPDLVRQTLDKVR
                     QEGLSATMRAVRRKLGESIALGYSLSGEVLAVGERVEGLRPGDLVAAGGGGYALHADV
                     VVVPENLVVRVPDGAALDAAAFATIGAVSMHGFRLGKTEVGEVVAVVGLGLIGLLAGA
                     IAKAAGCRVIGIDLRPEALERALTMGFDDAVPAGDDAARRVDAASRGRGADAVLLCAS
                     SSSNAPVLLAGEIARDRARVVVVGDIPVEGPREVFFRKELELVVSRSYGPGRYDPAYE
                     EKGQDYPVGYVRWTQRRNMEAFVDLVAAGGMDVLGTVTHRFPVEKADDAYATLETTAG
                     AVVLLEYPAEERRAPAPVTAPAPARPGARILRVGLVGTGSFASRVLVPAIASDEGAAI
                     AAVAALQGRPPSIDGARHAASPQEVFADPEVHAVFVATRHDSHSELAAAALAAGKAVF
                     VEKPLALSHQELDAVVAARAGSSGPLMTGFNRRHAPFTERLRSLTGSGAPAVIYARVN
                     AGPLPPDHWTKDLDVGGGRILGEMCHFVDLVTYLAGSAPTTVAAQAVAGGSAQAAQDV
                     VATISFADGSVATVLYTAVGDPASGKERIEVFCGGRTGIIDDWRTLTLVSGGRSAEEK
                     GRMDKGHAAEVASFLKAVRDGASLADSFARDVQSTRATLAVVESLATGLPVALRR"
     gene            complement(5381..>6061)
                     /locus_tag="VEV43_03680"
     CDS             complement(5381..>6061)
                     /locus_tag="VEV43_03680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYP22652.1"
                     /translation="SLDAMGDFPLLSLLKEPLNDANRFAKRAMDLLIATVAMTVAAPV
                     MLVTAIAIRVTSKGPILFRQQRIGMDGRPFDILKFRTMVTGADSSIHQTFVEGLIRGD
                     VAQDEDVEMYKLTDDPRVTKVGWFLRRTSIDELPQLFNVLRGEMSVVGPRPPLSFEVE
                     MYQEWHRRRLEVRPGMTGLWQVSGRSKMTFDEMVRLDIHYIETWSPLRDVSIVLRTIP
                     ALLRKEAS"
BASE COUNT          906 a         2223 c         2077 g          855 t
ORIGIN      
        1 gcgttcggtg ttgtcgcgca gcgtcagaca cggcacgccg agcacgacgg cctcctcttg
       61 gatcccgccg gagtccgtca gcacgagcgc ggcccggctc atcagcgcgg tgaagtccga
      121 gtacgagagc gggggcaggg acctgactcc cgtcgcgtcg aacgagaggc cgagagcgtc
      181 caacctcttc ttggtccgag ggtggaccgg gaacagcagc ggcagcctct cggcgacgtc
      241 cgccagcgct ccgacgagac ccttcagcac cgcgtcgtcg tccacgttcc ccgcgcggtg
      301 cagcgtgacg agcccgaacg cgtcgccctc cccgaggtcg tagtcggcga gcaccctctg
      361 ggcgtcgagc cgcggcagga tccagtcgag cgagtcgatc attacgttgc cgatccggtg
      421 gatcttggcg cgctcgatcc cttcggctcg caggttgtcg tccgcgtcct ccgacggagt
      481 gagcaggaac tgcgagatgc ggtcgacgac gatgcggttg cgctcctccg gcatcgacca
      541 gtcacggctt cggagccccg cctcgacgtg agcgagcggg acgcgggcga acgacatcgc
      601 gagcgacgcc gcgagcgtgg aagtgacgtc acccacgacg acgcccgcat cgggccgcag
      661 ctccgcgagg acgggctcga ggcgcgtgat gatctgcgcc gtctgccagg actccgtccc
      721 cgacccgacc tcgaggtgga cctcgggccg cgggagttcg aggacgcgca ggaactgatc
      781 cgacatcgcc tcgtcgaaat gctggcccgt gtgcacgagc acgggctcga agccgccgcg
      841 ctctctcatc tcgcggaaga tcggcgcgat cttcatgaag ttcggcctcg ccccgaacac
      901 gagcatcacc ttggtcacga aaacgtcttc tccagcagcg tcactacgtc ggtgacgcga
      961 tgctcgggtg gcgtccactc gacgcgcggc ttccgcacga cagggatcgc cgcgacggct
     1021 gccgcgtcgg acacgcggac gaggcggccg gactcgatca aggcgccgtc cacggcggcc
     1081 ggcttgcccg agaaaagcgt gtagctgggc gtgccgagga tggccgcctc gcgcgtcatc
     1141 gtgccgcccg cgcccacgac gagatccgac gcccacagga ggtcgaggcc gctgacggcg
     1201 tgctccagga cgcgcacgcc ctcgatcgtg ctgtagctgc gcccctgctc gacggtgcgc
     1261 ggcgagagca ggaccgtcgc gccttgcgcc gcaaccgtct cgatcgcgcg ggcgaagagg
     1321 tcgttcgaat gccggtggta gagcgcaccg tcgggcgggg gccgcgacgc gaccagcacc
     1381 tcgtcgtcgg tcacgccgag cgaggatcgg aaccccgcct cggggcggaa caccgccagc
     1441 gtgatctcct ccttgaaccc gtcgtagcgc aggagcttgc ggcgcgcgcc catcttgcgc
     1501 gcggcgtcct cggggaagtg cgcgggcacg actacgtcgt gggcgaggcg gaacgcgagg
     1561 tggttcgccg gctgatgctc gtagtccatc atcgtgacgg cgcgcgtgcg gagcagcagc
     1621 cgggccgcga tcacctggct gtaggagttg tgaccgagcg cgacgtcggg cctcgcgtcg
     1681 cggaccgcag ccgcgaggcc gcgcgcgcgc ttccagatcc ggaggctctt ctggacgacg
     1741 ctcctgtcgc ttccggcccc cacgacctcc acctccggcc agtaccgccg cgcgagatcc
     1801 gcggtctggg cgcgatccca ggcggtcagg atcacctggt gcccgcgttc acgcaggacg
     1861 tccacgaccg gcttgaagaa agtcgcgtgc ggtgagttcg cgatgtcgac ccacacgcgg
     1921 ctcacgcggc cgcccccacg ctccgcctct gcgcgacgag cgcggtcagc gcgagcgcca
     1981 tcggggcgtc cgaccagcgc gcgtacggga cgcgcttccg gtgcaacctc ccccgctggt
     2041 acgagaacga gccgttgcgc atgcgcatgc cacgcatcca ggccgcgatc cgttccgcga
     2101 gcgcgaggtc gcccagcttc gcgaacgtct ggatgccctg ggcccccgct atcgcgtcca
     2161 gtgggaaggg acgcccgggc gccggcaacg gctcgcccgc ggggccgaac aggtgctcgg
     2221 cgtaatagcg cgcggcgcgc gtgagcgggg cgacgacgga ggggaacgtc gcgcggacgt
     2281 cgtgcaggca ctcgaccacg aagcccgtgt gatgaccgtc gacccatctc acgtccggat
     2341 cctcgccgta gaggaacgag ccgtcgtcgc gctggtatcg gagcgtcgtc tccacggccg
     2401 ccgacgcggc gtcggcgagc gacccgtctc ccgacaagga tgcgctgcgg gtgcacagcc
     2461 tggcgccgag caggttcgcg ttgtgaatga gcttcggctc gccggggagg tacgcgaacc
     2521 agaagcgccc cccgcgttcg acccaaaagt cgcggcagat catgcgcgcc gcgcggactg
     2581 caccgtccag ccagcgttcg tcaccggtcg cctcggctgc gtcgaggaac gcgttcgcgg
     2641 cgaacgccgt cgacacgatg ttcgggtcgg tcttcggata gaagaacgtc ttcgtctgca
     2701 cgtcgaacgg atatccccag ccctcgccga cgcgggccga ctcgagccag gcgaggcctt
     2761 cctccgcaat gcgccggtgc accggctcgc cgagcagctg ccacagggtc agccgcgccg
     2821 acgcgaacag cccttcgccg tacgcgttgc gcgacggcgc gatcctgaac agcggccgca
     2881 ggttgacggg agagcgtttg tgcacctgca cgagcgcgag gcgcgcgacc cgcccgagcc
     2941 gtcgcggcga cgacacccac ctcgtctcga gcccgtcgta ggggtcgtac gtcctgccgt
     3001 cgcgcccgcg ccaacgttcg agcaacgcga ccgagatcgc gagcgcctcg tcgtcggtca
     3061 tcggcgaagc gcgacgggca gccccgtcgc cagtgactcg acgacggcga gcgtcgcgcg
     3121 cgtcgactga acgtctcgtg cgaacgagtc cgccagcgag gcaccgtcgc ggacggcctt
     3181 caggaacgac gcgacctcgg cggcgtgccc cttgtccatc ctgcccttct cctctgcgct
     3241 ccttccgccg gacacgagcg tcagcgtcct ccagtcgtcg atgatcccag tcctgccccc
     3301 gcagaagacc tcgatgcgct ccttgccgct cgccggatcg ccgaccgcgg tgtagaggac
     3361 cgtcgcgacc gacccgtccg cgaacgagat cgtcgccacg acgtcctggg cagcctgcgc
     3421 cgacccaccc gcgaccgcct gcgcggcgac cgtcgtgggg gcgctcccgg ccagatacgt
     3481 cacgaggtcg acgaagtgac acatctcgcc gaggatgcgg ccgccaccga cgtccagatc
     3541 cttggtccaa tggtccggag gcagcgggcc ggcgttgacc cgcgcgtaga tcaccgcggg
     3601 cgcgcccgac ccggtgagcg accgcaaacg ctccgtgaag ggcgcgtgcc gccggttgaa
     3661 gcccgtcatg aggggtccgc tcgacccggc ccgcgcggcg accacggcat cgagctcttg
     3721 gtggctcaac gcgaggggct tctcgacgaa gaccgccttg cccgcggcga gggccgcggc
     3781 cgcgagctcg ctgtgcgagt cgtgtcgcgt cgcgacgaac accgcgtgca cctcggggtc
     3841 ggcgaagacc tcctggggcg acgccgcgtg gcgcgcgccg tcgatcgacg gggggcgacc
     3901 ctgcagggcg gccacggccg cgatagcggc cccctcgtcc gaggcgatcg ccgggacgag
     3961 aacccgcgac gcgaagcttc ccgtcccgac gagcccgacg cgcaggatcc gcgcccccgg
     4021 cctcgccggt gcgggcgcgg taaccggcgc gggagcgcgc cgctcctccg ccggatactc
     4081 gagcaggacg accgcccccg cggtcgtctc gagcgtcgcg taagcgtcgt cggccttctc
     4141 gaccgggaag cggtgggtga cggtgccgag cacgtccatg ccgcccgcag cgacgaggtc
     4201 gacgaacgcc tccatgttcc gccgttgtgt ccaccggacg tacccgacgg gatagtcctg
     4261 acccttctcc tcgtacgccg ggtcgtaccg ccccggcccg tacgaccggg acacgacgag
     4321 ctcgagctcc tttctgaaga agacctcgcg cggcccttcg accgggatgt cgccgacgac
     4381 gacgacacgg gcacggtccc gggcgatctc gcccgcgagc agcaccggcg cgttcgacga
     4441 cgaagacgca cacagcaaga ccgcgtcggc accgcgtccg cggctggcgg cgtccacgcg
     4501 ccgggcggcg tcgtcacccg ccggcacggc gtcgtcgaac cccatcgtca gcgcgcgctc
     4561 gagcgcctca ggcctcaggt cgatcccgat aacgcggcac ccggcggcct tcgcgatggc
     4621 accggcgagg agaccgatca gcccgaggcc cacgaccgcc accacctcgc cgacctcggt
     4681 cttgccgagc cggaaaccgt gcatgctgac ggcgccgatc gtcgcgaacg cagcggcgtc
     4741 cagcgccgcg ccgtcgggga cgcgtacgac gaggttctcc ggcacgacca cgacgtcggc
     4801 atgcagcgcg taccccccgc cccccgccgc cacgaggtcg cccggacgca gcccttcgac
     4861 ccgctcgcct acggcgagca cctctcccga cagcgagtat ccgagcgcga tcgactcgcc
     4921 gagcttgcgc cggacggctc gcatcgtcgc ggacaggccc tcctgccgta ccttgtcgag
     4981 cgtctggcgc acgaggtcgg gccgggagcg cgccttgccg accagggtct tacgcgacag
     5041 ctcgagcttc gagcgttcgg tgcccgggct cacgacggag aacgcggtct ggatcagcac
     5101 ctcgcccggg cccagagcgg gggcgtggcc gagctcgacc tcgaccttgg cccccttcgg
     5161 ggacaggacg acgcggcgga ccggctgcgc cgtcgcctgc tcgtcgtcgt ctcggcggac
     5221 cggctgcgcc gtcgcctgct cgtcgtcgtc tcggcgggga gtggtggtca tgactgatct
     5281 cgctcgtttc ggtctgtcgg atgggcggcc gcagcgcgcc ggcagcggcc gcgggcgacc
     5341 ggcggctcgg gaggtcgacc ctaacgccct cgcgcacccg tcacgaggct tccttgcgca
     5401 gcagcgccgg gatcgtacgc agcacgatcg agacgtcgcg tagcggcgac cacgtctcga
     5461 tgtagtggat gtcgaggcgc accatctcgt cgaacgtcat cttcgaccgc ccgctcacct
     5521 gccacaggcc ggtcatcccg ggacggacct cgagccggcg ccggtgccat tcctggtaca
     5581 tctcgacctc gaacgacagc ggcggccggg ggcccaccac gctcatctcg ccgcgcagca
     5641 cgttgaagag ctgggggagc tcgtcgatcg aagtccgtcg caggaaccat ccgaccttcg
     5701 tcaccctcgg gtcgtccgtg agcttgtaca tctcgacgtc ctcgtcctgc gcgacgtcac
     5761 cgcggatcag accttcgacg aacgtctggt ggatgctcga gtccgcgccg gtcaccatcg
     5821 tgcggaactt caggatgtcg aacgggcggc cgtccatccc gatcctctgc tgccggaaca
     5881 ggatcggtcc cttcgaggta acgcgtatgg cgatcgccgt caccagcatg accggggccg
     5941 cgaccgtcat cgccactgtc gcgatcaaga ggtccatcgc gcgcttcgcg aagcggttgg
     6001 cgtcgttcag gggttccttc aacaggctca gcagcgggaa gtcgcccatc gcatcgaggc
     6061 t
//
LOCUS       DASQDO010000121         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_685150, whole genome shotgun sequence.
ACCESSION   DASQDO010000121 DASQDO010000000
VERSION     DASQDO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685150"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VEV43_03685"
     CDS             complement(<1..585)
                     /locus_tag="VEV43_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP22653.1"
                     /translation="MSPWHTGAGASGVVSACRHLVQGLALHPDAGRVVVLSLGAERRV
                     ESLWDGAVRVETIPRQTRLALATAGWPDYVRASRWVSATGFRPGVVHGQGLAVEGLLA
                     TRLARRLSVPAVVTVHGMVDARSGFSREAARAGLARRVMNETLARAAGIVFVSPFRKE
                     ELVGERTIETRVIPNAIAPEAFEVTRRSGANVILY"
     gene            complement(606..2003)
                     /locus_tag="VEV43_03690"
     CDS             complement(606..2003)
                     /locus_tag="VEV43_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYP22654.1"
                     /translation="MTTYRRAFPDTAIAAAAGLLAVVLGMVVVKSPMLAIAAAGLTVL
                     IVAGAVDPTLPAIAWLAVAPFVQAIEPGSPVLALTIAFHRALLPIAAVGTLFGEALRR
                     RLRLTVPERFLLLFLAYALVSLFITWRGQFSTPEGAEAVRTFLFAHVVPFGALVIASR
                     LPASSHRKVFVMLTVVAAVSAVGALVQSVAGVAVFPGGAVWQEIWDPRAAGALANPAV
                     SGYVAHVGTFVAIYLGFRYAALRLFAFGVAAAGAVFTVLTYTRSAWVAFALGLATIAW
                     LYRKARPWVIVFSVTALAAFTFNVGGFVDTAFLEERAGNRENAAGRVAFGSTGFRMFK
                     DQPLVGQGFGAYDTNARQFASGFGQVGAAVAFRETSHNSFLTILAELGAAGFLMYAGA
                     CYFGIRSSVRALRRGGPDVDRLLIVALLAGLVSYFVSANLIDMRFFSFAVSLFWFNVG
                     LTSAVVERPPETSPR"
     gene            complement(2000..3517)
                     /locus_tag="VEV43_03695"
     CDS             complement(2000..3517)
                     /locus_tag="VEV43_03695"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HYP22655.1"
                     /translation="MRGVPGAAAERIVSHSLAGTRWMLWLSATALACGFGTNVVLGRA
                     GPGLLGFYGFLTLALSLLGTFFVFGGGNVIVNYLPKLAPADKTSFLASYAGVVAVSAV
                     ALMAVVAMVPAVPDLVFGDSAPSGAVTYLTLLLPIAVLQMLAWSTLQADLALRTFAVS
                     QALVSSLTFAALVVAGAAGWLSEAAPRRYIVATVLLGNVASVAVAGVRLWRRQLRAWG
                     PARRWLLPEGFWGFSLYFQGGVMVSFLVRSVDQVYVLRELGLVSLGYYRSALVLSQLA
                     GFLLVVTDKGFYSTFCNVEPADHPQLHRRFVRLNSLGTALAGLVLMLFAGELLRPFGE
                     SATSESKTLVMLIAGAVLLALPIASVNNSLVTARERMRPIFVNNAAGAVVAVVLYALL
                     GRRFGLVGVGWAFLALQLVMLLLSSITVRRVAGVAFPLGAWAISVASVVIGAGAGSVL
                     DGSTLVEVGARCGVLLAFVPLALLVRLVTPAELKDLVALVVPGRWSPLRTRSAAS"
BASE COUNT          704 a         1361 c         1267 g          435 t
ORIGIN      
        1 gtacaggatc acgttcgcgc cgctccggcg cgtcacctcg aacgcttcgg gagcgatcgc
       61 gttcgggatc acgcgcgtct cgatcgtccg ctcgcccacc agctcctctt tgcggaaagg
      121 cgagacgaaa acgatcccgg cggcgcgcgc gagggtctcg ttcatgacgc ggcgagcgag
      181 cccggcgcgt gccgcttcgc gggagaaccc cgacctcgcg tcgaccatcc cgtgcaccgt
      241 gacgacggcc gggacgctca gccgccgcgc cagcctggtc gccaggagac cttcgaccgc
      301 cagcccttgg ccgtgaacca cgcccggccg gaaccctgtc gcggagaccc agcggctcgc
      361 ccgtacgtag tctggccatc ccgcggtcgc cagcgccagc ctggtctgcc gggggatcgt
      421 ctcgaccctc accgcgccgt cccagagcga ctccactcgt ctttccgcgc cgagagagag
      481 caccaccact cggcccgcgt cggggtgcag cgcgagtccc tgcacgaggt ggcggcacgc
      541 ggagacgacg ccgctggcgc cggcgccggt gtgccacgga ctcaccaata ggacgtctag
      601 aagtgtcatc gcggggacgt ctcaggcggt cgttccacga cggcactggt gaggccgacg
      661 ttgaaccaga agaggctgac cgcgaacgag aagaacctca tgtcgatgag gttggcgctc
      721 acgaagtagg agacgaggcc ggcgaggagc gccacgatca acagacggtc gacgtccggc
      781 cccccgcggc gcagcgcgcg caccgagctc ctgatgccga agtagcaagc ccccgcgtac
      841 atcaggaatc ccgctgctcc gagctcggca aggatcgtga ggaaggagtt gtgcgaggtc
      901 tcgcggaagg cgaccgcggc gccgacctga ccgaaccccg aagcgaactg ccgcgcgttc
      961 gtatcgtagg cgccgaaccc ctggccgacg aggggctgat ccttgaacat gcggaacccg
     1021 gtcgatccga acgccacccg ccccgcggcg ttctcccggt tgcctgctct ctcttcgagg
     1081 aaggccgtgt cgacgaagcc tccgacgttg aacgtgaaag cagcgagggc tgtcaccgag
     1141 aagacgatca cccacggacg ggccttcctg tacagccagg cgatcgtcgc gagccccagg
     1201 gcgaaggcga cccacgcgct gcgtgtgtag gtgaggacgg tgaagacggc acccgcagcg
     1261 gcgacgccga acgcgaagag gcgcagagcc gcatacctga acccgaggta gatcgcgacg
     1321 aacgtcccca cgtgcgcgac gtatccgctg acagccgggt tcgccagcgc tccggcggcc
     1381 cgcgggtccc atatctcctg ccatacggcg ccgccgggga agacggcgac tccggcgacg
     1441 ctttgcacga gcgcgccgac cgcgctgacc gcggcgacca ccgtgagcat gacgaacacc
     1501 ttgcggtgcg acgacgccgg cagcctggat gcgatcacca gcgcaccgaa cggcacgacg
     1561 tgcgcaaaca ggaacgtccg cacggcctcc gcgccctcag gtgtcgagaa ctgtccgcgc
     1621 catgtgatga agagagagac gagcgcgtac gcgaggaaga gcaggagaaa cctctcgggt
     1681 accgtcaatc gcaggcgccg tcgcagcgcc tcgccgaaga gcgtcccgac agcggcgatc
     1741 ggcagcagcg cgcggtggaa tgcgatcgtc aacgcgagca ccggcgaccc gggctcgatc
     1801 gcctggacga aaggcgcgac cgccaaccag gcgatcgccg ggagcgtcgg gtcgacggct
     1861 cccgccacga tcaagaccgt gaggcccgct gccgcgatcg cgagcatcgg gctcttcacc
     1921 acgaccatgc cgaggacgac cgcgaggagc cccgcggcgg cggcgatcgc tgtgtcggga
     1981 aacgcccgcc tgtaggtcgt cacgaagcgg cgctcctggt ccggagggga gaccatcggc
     2041 cggggaccac gagggcgacg agatctttca gctcggccgg cgtcacgagc ctgaccagca
     2101 acgcaagagg gacgaacgcc agcaagaccc cgcagcgcgc gcccacctcg acgagcgtcg
     2161 agccgtcgag gaccgagccc gcccccgcac cgatcaccac cgatgcgacc gatatcgccc
     2221 acgcaccgag ggggaacgct acgccggcga cgcggcgaac cgtgatgctc gacagaagca
     2281 gcatgacgag ttggagtgcc aggaacgccc agccgacgcc gaccaggccg aagcggcggc
     2341 ccagcagcgc gtagaggact acggccacca cggctcccgc ggcgttgttc acgaagatcg
     2401 gccgcatcct ctccctggcc gtcaccagcg agttgttcac gctcgcgatc gggagcgcga
     2461 ggaggaccgc gcctgcgatc agcatgacca gcgtcttcga ttccgacgtc gcgctctcgc
     2521 cgaacggccg cagcaactct cccgcgaaca gcatcaggac gagaccggcg agtgcggtcc
     2581 ccaaggagtt cagccgcacg aaccggcgat gcagctgcgg atggtcggcc ggctcgacgt
     2641 tgcagaaggt gctgtagaaa cccttgtcgg tcacgaccag gaggaagccg gccagctgcg
     2701 acagcaccag cgcgctgcgg tagtagccga gcgacacgag gccgagctct cgcagcacgt
     2761 agacctgatc gacgctgcgt accaggaacg agaccatgac gccgccctgg aagtagaggc
     2821 tgaatcccca gaaaccctcg gggagaagcc atcgccgcgc cgggccccac gcacgcagct
     2881 gccgcctcca cagccgcaca cccgctaccg cgaccgacgc gacgttcccc agcagcaccg
     2941 tcgccacgat gtaccggcgc ggagccgcct ccgagagcca tcctgctgcg cccgccacga
     3001 ccagcgccgc gaacgtcagg ctcgagacga gcgcctggga caccgcgaac gtccgcagag
     3061 cgaggtccgc ctggagggtc gaccacgcga gcatctggag aaccgctatg ggaagcagca
     3121 gcgtgagata ggtcaccgct cccgaggggg cgctgtcgcc gaacaccagg tcggggaccg
     3181 ccgggaccat ggcgacgacc gccatcagcg ccacggcgga tacggcgacg acacccgcgt
     3241 agctcgcaag gaacgacgtc ttgtcggcag gtgcgagctt cgggaggtag ttgacgatga
     3301 cgttgccgcc cccgaagacg aagaacgtcc cgagcaacga aagggcgagg gtcagaaacc
     3361 cgtagaaccc gagcagcccc ggacccgccc gacccaggac gacgttcgtg ccgaacccgc
     3421 acgcgagcgc ggtcgccgat agccacagca tccaacgcgt gcccgcgagc gagtgcgaga
     3481 cgatcctctc cgccgcggcc ccagggacgc ccctcacccc cgagatcggc gcggcggact
     3541 cgccggcgcc cggccgcgcc ggccgatctc cgacgtgcgc gtctctctcc gtcacagcgc
     3601 agcggccaag acgtcgacga cgcggccggc cgctcgcccg tcccacaggg gcggtcgcgt
     3661 cgccgcgtgc cgggggccgc cgagcgcctc gtccgcgagc acgacgaccc gcgcgggatc
     3721 cgtccccgcg agccggttca gcccggtggc cagcgttatc gggcgtt
//
LOCUS       DASQDO010000122         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_746966, whole genome shotgun sequence.
ACCESSION   DASQDO010000122 DASQDO010000000
VERSION     DASQDO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746966"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(178..272)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            300..1298
                     /locus_tag="VEV43_03700"
     CDS             300..1298
                     /locus_tag="VEV43_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HYP22656.1"
                     /translation="MPNLPKPRFGRPVRSRGRIAGPADAETEVPNVEIVEAEGLRWIH
                     IERPRQADRTWLEEHFDFHPLDYEDIFSRNQRPKIDEYDDYLLVVLHFPRFDKEVGRL
                     NTAELDLFVGPDFVITLPNAPIQPLEYLFERCRTREDLRDSLFAKGPGYLLYKIVDDC
                     VDASFPMLAKMGNKLEVLEDEIWEGRSHEIVRDISNVKQEIINFRKIVRPQRAALRDL
                     ERTKRYIPEGLDIYFDDINDASERIWDMLENFKEVVEGLESTNESVLSHRVNDVLKAL
                     TAISVVVLPLTLVASIWGMNVGVPGEGSLHAFWIVVGAMVALLVAMLMFFRKRGWL"
     gene            1309..>1641
                     /locus_tag="VEV43_03705"
     CDS             1309..>1641
                     /locus_tag="VEV43_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HYP22657.1"
                     /translation="MTALEPLVRLAEAAALGAPVQRRRMLVIANPSATTMTDRTRNLV
                     VAALHRRYEVEAVDTQARGHATELCLDAAKEGYDVIVSFGGDGTVNEVANGLIGCATP
                     VTCLPGGAT"
BASE COUNT          272 a          572 c          525 g          272 t
ORIGIN      
        1 ccgacgtcgg tgatcttcgg gtggtcgtag cccacctcca gcggtccgaa gcacttcgag
       61 cacacgtagc gcgcctcgag cgcgtattcg gtcccgcatt ccctgcactt cagctgcttg
      121 gcagccatat gtccccttcg ccgtgttgga ttccgtggcg acggcgcaat ggcctgtgcc
      181 acatctaaca ggaattggca ccgtttcccg cgaactgcgg gttggttgcc ggggcttcgt
      241 agggcctgtc cctccacccc tctggatgtg tccagctctc gaccggcaca atatcagcca
      301 tgccgaacct tccaaagcca cggtttggcc gccccgtccg cagccggggg cggatcgccg
      361 ggcccgcgga cgccgagaca gaggtcccga acgtcgagat cgtcgaagcc gagggcctgc
      421 gctggatcca catcgagcga ccgcgccagg ccgaccggac gtggctcgag gagcacttcg
      481 acttccatcc gctcgactac gaggacatct tctcgcgcaa ccagcgcccg aagatcgacg
      541 agtacgacga ctacctgctc gtcgtcctgc actttccgcg cttcgacaag gaggtcgggc
      601 gcctgaacac cgccgagctc gacctgttcg tcggcccgga cttcgtcatc acgctgccca
      661 acgccccgat ccagccgctg gagtacctct tcgagcgctg ccgcacgcgc gaggacctgc
      721 gcgactcgct gttcgccaag gggccgggct acctgctcta caagatcgtc gacgactgcg
      781 tcgacgcgtc gtttccgatg ctcgcgaaga tgggcaacaa gctcgaggtg ctcgaggacg
      841 agatctggga ggggcgctcg cacgagatcg tgcgcgacat ctccaacgtc aagcaggaga
      901 tcatcaactt ccgcaagatc gtccgcccgc agcgcgcggc gctacgcgat ctggagcgca
      961 cgaagcgcta catcccggag ggcctggaca tctacttcga cgacatcaac gacgcgtcgg
     1021 agcgcatctg ggacatgctc gagaacttca aggaggtcgt cgagggcctc gagtcgacga
     1081 acgagtcggt gctctcgcat cgcgtcaacg acgtcctcaa ggcgctgacc gcgatcagcg
     1141 tcgtcgtgct gccgctgacg ctcgtggcga gcatctgggg aatgaacgtc ggggtgccgg
     1201 gcgaggggtc gctgcacgcg ttctggatcg tcgtcggcgc gatggtcgcg ctgctcgtcg
     1261 ccatgctgat gttcttccgc aagcgcggct ggctgtagaa caccgggcat gaccgccctc
     1321 gaaccgctcg tccgcctcgc cgaggccgcc gctctcggcg cgcccgtcca gcgccggcgc
     1381 atgctcgtca tcgccaaccc gtcggcgacg acgatgaccg accgcacgcg caacctcgtc
     1441 gtggccgcgc tgcaccgccg ctacgaggtg gaggcggtcg acacgcaggc gcgcgggcac
     1501 gccaccgagc tgtgcctcga cgcggcgaag gagggctacg acgtcatcgt gtccttcggt
     1561 ggcgacggaa ccgtcaacga ggtcgccaac gggctgatcg gatgcgcgac gcccgtcacc
     1621 tgcctgcccg gcggcgcgac g
//
LOCUS       DASQDO010000123         9222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_685547, whole genome shotgun sequence.
ACCESSION   DASQDO010000123 DASQDO010000000
VERSION     DASQDO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9222
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685547"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..787
                     /gene="ccsA"
                     /locus_tag="VEV43_03710"
     CDS             47..787
                     /gene="ccsA"
                     /locus_tag="VEV43_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYP22658.1"
                     /translation="MSPSRRLTVLGYAAWGSIALSLALVFLLARTADPSMGGELQRIF
                     YFHVPAAWIAYLAFAIVFIASIAYLRTGSDRWDLLAGSSAEVGVVFCTLVLITGPIWA
                     RPVWGTWWQWDARLTSALVLWLTYVGYLFLRALSTEPGRTGRLAAVVGIVGFVNVPIV
                     HFSVRWWRTLHPSGPTPANPAEASGLGGPEMTAFFVSLAAFTLLFAWLLATRVRLGRL
                     EAEVDALELARARAELSPRPEPTPAPVT"
     gene            784..912
                     /locus_tag="VEV43_03715"
     CDS             784..912
                     /locus_tag="VEV43_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HYP22659.1"
                     /translation="MNDVAWLFVAFTAVWVALGAYLLSIATRQRRLEKRLDELRPK"
     gene            1055..1426
                     /locus_tag="VEV43_03720"
     CDS             1055..1426
                     /locus_tag="VEV43_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22660.1"
                     /translation="MAMRERIYKVTDAPMTWTRAIVLGLVLFVLATMLLGQAPSWIIY
                     WFDAQVAQIIEFSAKIPGVNPEGLNAKQVAIVRDVVANAVQNTFLIAFLAGAYFWQKS
                     KQKRTGAKGPTDPVKGYMPGK"
     gene            1432..1854
                     /locus_tag="VEV43_03725"
     CDS             1432..1854
                     /locus_tag="VEV43_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYP22661.1"
                     /translation="MPTTPEPKKMEIIEKPIFRADYEMEMVDADWLKGAVKPKRFIDF
                     IPEQCILCEGCVDICPWYCIFMVDPTAVEESGNVELARQMTGQDILFIIDDNACTRCN
                     LCVERCPTGALVQSEVQGGKKKTRARALGTVSETQRAS"
     gene            1876..2652
                     /gene="extP"
                     /locus_tag="VEV43_03730"
     CDS             1876..2652
                     /gene="extP"
                     /locus_tag="VEV43_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HYP22662.1"
                     /translation="MAKAEGRFNPREKMSAVMDTLHGSQLWRAIFRPGSIFRRGYADS
                     PRNRAYVVMNSLLYHLHPVKVKRHGIKLSYTLCLGGLSFFLFILLTITGIYLMFFYRP
                     SVEVVYSDMQRIHTQVSFGLLVRNLHRWGAHLMTISVFLHMARVFYTGAYKPPREFNW
                     IVGVILLLLTLLLAFTGYLLPWDQLAVWAVTVGTEMMKNTPVIGDQVQFALLGGPVIG
                     AETLLRWYTLHVLLLPFVIVIFISIHFWRVRKDGGISGPM"
     gene            2668..3495
                     /locus_tag="VEV43_03735"
     CDS             2668..3495
                     /locus_tag="VEV43_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HYP22663.1"
                     /translation="MPTPDEVYEQVLAEETAKGSSPAVAQARAKAARVRASRGAKDPS
                     KPDSATAPRKAPGGEAEAKPAAAAAEAKPAAAATAKPAAAAARPAARAAGTGGGAKKA
                     VGNGGPAVPERVQRLLAVVKPEAIQKVERRPMDRVNVWPHLMAAEFVSLLVVLAAVTF
                     FSIAVDAPLRELSNFNQTPNPSKAPWYFLGLQELLRYFHPQVAGVTIPQVIIIILFAF
                     PFIDRNPSTRPDDRKLAIVVFSFFMLSFAVLTTIGMFFRGPGFNWVYPWESGIFFEL"
     gene            3533..4342
                     /locus_tag="VEV43_03740"
     CDS             3533..4342
                     /locus_tag="VEV43_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYP22664.1"
                     /translation="MSPGTIALLVVAASILAFALFMLNVTIRRQRQARSEAGHGQSGP
                     PSTDLDPKPSVRPQPAQSKDVTVETVPKAQRKPLTAEQMAISRRQFLNRSWTASFAAF
                     LGFFGMGSLSFLWPKLTGGFGTRINAGNYDDILSQVGPEGGFKPLFIPEGRFYLVYYE
                     GTGESPVYAATGAVETKLQALYRKCVHLGCSVPHCDTSMLFECPCHGSKYQLTGEFFA
                     GPAPRGLDRFPVAVEGTAVMVDTGSIQTGPPRGTSTWERFAEPTGAYCVPV"
     gene            4417..5394
                     /locus_tag="VEV43_03745"
     CDS             4417..5394
                     /locus_tag="VEV43_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYP22665.1"
                     /translation="MVIVPSALMWAIFLARSGRPGRGPRPQIGIPQALRPGQPDEQLE
                     GPRLDRILAGGVMATVALAIFVPAYWLPEAERQASFQERFDEESLHRGELIFADPPDL
                     PEGAAASEYKELERQISLGMNCAQCHGPEAGGGLAQRTDPETGEELTWQAPPLTTVFH
                     RWDEEIVKFTIERGRPGTPMPAWGVEFGGPMTEQMINDVMAWLKSIQEPPPELAEACN
                     DPLADDALSCSSGKEIFEARCTVCHGDRGQGREADGTDEDPILPGLALWKGDVRHLDK
                     GQHLFTIRNGRRFAFMPQFSEAPAQGIPIPPYPLTEKQIEAVLEYERTL"
     gene            5395..6156
                     /locus_tag="VEV43_03750"
     CDS             5395..6156
                     /locus_tag="VEV43_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22666.1"
                     /translation="MDSIFVEGVAATVSSIIVFCGSVWLLLALVLGPRLSYFITASVT
                     LGFLLMMGVVWSVGEPLGPVGELPSYSQVGVGDTPAESGFGPAGDFPDGGGWFTPNPE
                     EAAHAEIKSGAEGEAPDLLAEAIDAGEITSFEQPDQAQVDADSTQLLEQDGTFYAAVR
                     FEPVPSDPEEEDAASDQDIAPEEGDGPAEDDAAAQEEELDPNTEAYVFLEQDPGDPSG
                     QARMITLGFFVLLVLHLMGLSASERRAKRARAETS"
     gene            6163..6339
                     /locus_tag="VEV43_03755"
     CDS             6163..6339
                     /locus_tag="VEV43_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22667.1"
                     /translation="MVRTHMRTTATAGLLLLLSGCTRTGINAGEQGGQAFILFTVMLL
                     VTIAILWFILGREE"
     gene            complement(6317..6499)
                     /locus_tag="VEV43_03760"
     CDS             complement(6317..6499)
                     /locus_tag="VEV43_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22668.1"
                     /translation="MTMLIGALILLGGAILDTVADEVPTWLRVGIFFVGYVFLAYGFF
                     TALNARSGRSTPRGRG"
     gene            complement(6567..7874)
                     /gene="hemL"
                     /locus_tag="VEV43_03765"
     CDS             complement(6567..7874)
                     /gene="hemL"
                     /locus_tag="VEV43_03765"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HYP22669.1"
                     /translation="MAELKPQPLREASDKLLAEAVSLMPGGVNSPVRSFEAVGAHPFF
                     VASASGPYLTDVDGNTYLDFVQSWGALLFGHAHPAIVEAVTEAARKGTSFGTPSELEV
                     ELARLVVDLVPNVDKVRFVSSGTEAGMTAVRLARGATGRDKVLKFSGCYHGHVDALLV
                     DAGSGVATLGIPGTPGVTKGAAGDTLVAKYNDLESVRDAIDGFEDDLAAILVEPVAAN
                     MGLVLPEEGFLQGLRALADATGALLVFDEVITGFRLGRGGAQERFGVRADLVMLGKVL
                     GAGLPVAAIGGRSDVMDELAPVGPVYQAGTLSGNPLAMAAGIASLGLVQSDPGLYERV
                     QERARSLVKGLVDAADEAEVPMVAAAIGGLAGFFFAGEEVLDYEAAKKTDQGTYARFF
                     RGMLAHGVYLPPSRFEALFVSAVHTEEHVDQLVEAARGVFATR"
     gene            complement(7862..8815)
                     /gene="hemB"
                     /locus_tag="VEV43_03770"
     CDS             complement(7862..8815)
                     /gene="hemB"
                     /locus_tag="VEV43_03770"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYP22670.1"
                     /translation="MRRLRRTPGLRRAFAETTVFPSDLIAPLFVKEGIDDAVPISSMP
                     GHFQHTLETLVKEARELADRGVAGVILFGIPAAKDESGFEAWSPDGIVQRGLSALKDE
                     LGDEAVVIADLCLCEYTSHGHCGILSGETVDNDRTISLYGRIAASQAAAGADVVAPSG
                     MMDGQVGEIRAALDAAGHQEIPILAYAAKYASSFYGPFREAAEGAPQFGDRRAYQQDP
                     ANVAEALREVALDVAEGADAVMVKPALPYLDVLRAVKERFGLPTAAYNVSGEYSMLMA
                     AAERGWIDERAATLETLTSIKRAGADLILTYHAKKAAEWLS"
     gene            complement(8833..>9222)
                     /locus_tag="VEV43_03775"
     CDS             complement(8833..>9222)
                     /locus_tag="VEV43_03775"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYP22671.1"
                     /translation="PRVEGAPPDVVDALSSNGWSVREVAAYRNLPADRDTPGASAVAT
                     RRVDVVTFTSASTVRNFLQVAGTPQELGLETGGGGNRAVACIGPVTATAATEAGLRVD
                     VVAEEHSVPGLVRALVERFGHVAPLEP"
BASE COUNT         1408 a         3250 c         3140 g         1424 t
ORIGIN      
        1 gatgcacttc gagctcggaa gactgatcag cccgtaacct aggcgcatgt ccccgtcccg
       61 ccgcctgacc gtcctcggct acgccgcctg gggctcgatc gcgctctcgc tcgccctcgt
      121 cttcctcctg gccaggacgg ccgacccgtc gatgggcggc gagctccagc ggatcttcta
      181 cttccacgtc cccgcggcgt ggatcgccta cctcgccttc gcgatcgtgt tcatcgcgtc
      241 gatcgcctac ctgaggaccg ggagcgacag atgggacctc ctcgcggggt cgtccgccga
      301 ggtcggcgtc gtgttctgca cgctcgtgct gatcaccggc cccatctggg cccggcccgt
      361 gtgggggacc tggtggcagt gggacgcccg gctgacctcg gccctcgtcc tgtggctgac
      421 ctacgtcggg tacctgttcc tgcgggcgct ctcgaccgag ccgggacgga ccggccgcct
      481 cgccgccgtc gtgggcatcg tcgggttcgt gaacgtgccg atcgtccact tctcggtgcg
      541 gtggtggcgg accctgcacc cgagcgggcc gaccccggcg aaccccgccg aggcgtcggg
      601 cctggggggg ccggagatga cggcgttctt cgtgtcgctc gccgcgttca cgctgctgtt
      661 cgcgtggctg ctggcgacga gggtgcggct cggccgcctg gaggcggagg tcgacgcgct
      721 cgagctggcg cgggcgaggg ccgagctgtc gccacgcccg gagcccacac cggcccccgt
      781 gacatgaacg acgtagcctg gctcttcgta gcgttcaccg cggtctgggt cgcgctcggc
      841 gcataccttc tgtcgatagc gacgcgccag cgccggctgg agaaacgact cgacgagctc
      901 cgacccaaat agcctgctca cgcgccggag aaacctagct ttggaccatt cctaggccta
      961 gccgaacaat tggcgatgct ccgcccaaag ctaggttttt cactttcgga cttgtggccg
     1021 cggcccgggg gggcgccgtt aatatgacgc cctgatggcc atgcgcgaac gcatctacaa
     1081 agtgaccgac gcgcccatga cctggaccag agccattgtc ctggggctcg tcctattcgt
     1141 tctagcgacg atgctgttgg gccaggcccc ctcgtggatc atctattggt tcgacgccca
     1201 ggtggcccag atcatcgagt tctccgccaa gatccccggc gtcaaccccg agggcctgaa
     1261 cgccaagcag gtcgccatcg tccgcgacgt cgtcgcgaac gccgtgcaga acacgttcct
     1321 gatcgcgttc ctcgccggcg cctacttctg gcagaagtcg aagcagaaga ggacgggcgc
     1381 caagggtccg acggaccccg tcaagggcta catgccgggg aagtagggga catgcccacg
     1441 acgcccgagc cgaagaagat ggagatcatc gagaagccca tcttccgcgc ggactacgag
     1501 atggagatgg tcgacgccga ctggctcaag ggcgccgtca agccgaagcg cttcatcgac
     1561 ttcatccccg agcagtgcat cctctgcgag ggctgcgtcg acatctgccc ctggtactgc
     1621 atcttcatgg tcgacccgac ggcggtcgag gagtccggca acgtcgagct cgcacgccag
     1681 atgaccggcc aggacatcct cttcatcatc gacgacaacg cctgcacccg ctgcaacctc
     1741 tgcgtcgagc gctgccccac cggtgcgctc gtccagagcg aggtgcaggg cggcaagaag
     1801 aagacgcgtg cccgcgcgct gggtacggtg tccgagaccc aacgagcctc gtagacaagg
     1861 gaaagggtta gacgagtggc gaaggccgaa ggccggttca acccccgcga gaagatgtcc
     1921 gccgtcatgg acacgctcca cgggagccag ctgtggcgcg cgatcttccg ccccggctcg
     1981 atcttccgcc gcggctacgc cgactcgccg cgcaaccgtg cctacgtcgt catgaactcg
     2041 ctcctgtacc acctccaccc ggtgaaggtg aagcggcacg ggatcaagct ctcctacacg
     2101 ctgtgcctcg gcgggctctc gttcttcttg ttcatcctgc tgacgatcac gggcatctac
     2161 ctgatgttct tctaccggcc ctcggtcgag gtcgtgtact cggacatgca gcgcatccac
     2221 acgcaggtgt cgttcgggct tctcgtccgc aacctgcatc ggtggggcgc ccacctcatg
     2281 acgatcagcg tgttcctcca catggcccgc gtcttctaca ccggcgccta caagccgccg
     2341 cgtgagttca actggatcgt cggcgtcatc ctgctgctgc tgaccctgct cctcgccttc
     2401 accggctacc tgctcccgtg ggaccagctg gcggtctggg ccgtcacggt cggcaccgag
     2461 atgatgaaga acaccccggt gatcggggac caagtccaat tcgccttgtt aggcggcccc
     2521 gtgatcgggg ccgagacgct gctgcgctgg tacacgctcc acgtgttgct gctcccgttc
     2581 gtgatcgtga tcttcatctc catccacttc tggcgagtgc gtaaggacgg aggtatctcc
     2641 ggccccatgt aggggagagg aaccgcgatg ccgacacccg acgaggtata cgagcaggta
     2701 ctggccgagg agaccgccaa gggctcctcg ccggcagtcg cccaggcgcg tgcgaaggcc
     2761 gcccgggtcc gcgcctcgcg cggtgccaag gacccgtcca agcccgactc cgcgacggcg
     2821 ccgcgcaagg ctccgggggg cgaggccgag gccaagcccg cagccgcggc cgcagaggcc
     2881 aagccggctg ccgcggcgac cgccaagccc gcagctgccg cagctcgtcc cgcggcccgg
     2941 gcggccggga ccggcggcgg cgccaagaag gccgtcggca acgggggccc ggcggtcccc
     3001 gagcgcgtcc agaggctcct cgccgtcgtc aagcccgagg cgatccagaa ggtcgagcgc
     3061 cggccgatgg accgcgtcaa cgtgtggccg cacctcatgg ccgccgagtt cgtgtcgctg
     3121 ctcgtcgtgc tcgcggccgt gacgttcttc tcgatcgccg tcgacgcacc gctgcgtgag
     3181 ctctcgaact tcaaccagac gccgaacccg tcgaaggcgc cgtggtactt cctcggcctc
     3241 caggagctcc tgcgctactt ccacccgcag gtcgccggcg tcacgatccc gcaggtcatc
     3301 atcatcatcc tgttcgcgtt cccgttcatc gaccggaacc cgtcgacgag gccggacgac
     3361 cgcaagctcg cgatcgtcgt gttctcgttc ttcatgctga gcttcgccgt cctgaccacg
     3421 atcggcatgt tcttccgggg gcccggcttc aactgggtct acccgtggga gagcggcatc
     3481 ttcttcgagc tatgacgcag acctcgcacg accgcaaagg agttgagccc aggtgagccc
     3541 gggaaccatc gccctgctcg tagtcgcggc gagcatcctg gccttcgcgc tgttcatgct
     3601 gaacgtcacg atccgccgcc agcgccaggc gcgctcggag gcaggtcacg ggcagagcgg
     3661 cccgccgtcg acggacctcg accccaagcc gtcggtgcgt ccgcagcccg cacagagcaa
     3721 ggacgtgacg gtcgagacgg tgccgaaggc gcagcgcaag ccgctcacgg ccgagcagat
     3781 ggcgatctca cggcgtcagt tcctcaaccg ctcgtggacc gcttcgttcg ccgcgttcct
     3841 cggcttcttc gggatggggt cgctgtcgtt cctgtggccg aagctgaccg gcgggttcgg
     3901 gacgaggatc aacgccggca actacgacga catcctgtcg caggtcgggc cggagggcgg
     3961 gttcaagccg ctgttcatcc cggagggacg cttctacctc gtgtactacg agggcacggg
     4021 cgagtcgccg gtgtacgcgg cgacgggggc cgtcgagacg aagctgcagg cgctgtaccg
     4081 caagtgcgtg cacctcggct gctcggtccc ccactgcgac acgtcgatgc tgttcgaatg
     4141 tccgtgccac gggtcgaagt accagctcac cggcgagttc ttcgccggcc cggccccccg
     4201 gggcctggac cggttcccgg tggcggtcga gggcacggcg gtgatggtcg acacggggag
     4261 cattcagacg gggcccccgc gcggcacgtc gacctgggag aggtttgcgg agcccaccgg
     4321 cgcgtactgc gtgccggtct agggctgagg ctactggtgt ctaggaaggc aggggcggcc
     4381 taagtgtcat cgagcgacgt tctcaccctc gcgctggtgg tcatcgtgcc ctcggcgctc
     4441 atgtgggcga tcttcctcgc gcgcagtggg cggcccggac gggggccgcg tccccagatc
     4501 ggcatcccgc aggcgctccg gccggggcag cccgacgagc agctcgaggg cccgcggctc
     4561 gaccggatcc tcgccggcgg cgtgatggcg acggtcgcgc tggcgatctt cgtgcccgcc
     4621 tactggcttc ccgaggccga gcggcaggcg tcgttccagg agcgcttcga cgaggagtcc
     4681 ctgcaccgcg gcgagctcat cttcgccgac ccgccggacc tgcccgaggg ggccgcggcg
     4741 tccgagtaca aggagctcga gcggcagatc tcgctcggca tgaactgcgc gcagtgccac
     4801 ggccccgagg ccggcggcgg gctcgcgcag cgcaccgacc ccgagacggg cgaagagctg
     4861 acgtggcagg cgccgccgct cacgacggtg ttccaccgtt gggacgagga gatcgtgaag
     4921 ttcacgatcg agcgcgggag gcccgggacg ccgatgccgg cgtggggcgt cgagttcggc
     4981 ggccccatga ccgagcagat gatcaacgac gtgatggcgt ggctgaagag catccaggag
     5041 ccgccgcccg agctcgcaga ggcgtgcaac gacccactcg ccgacgatgc gctgtcgtgc
     5101 tcgtccggca aggagatctt cgaggcccgc tgcacggtct gtcacgggga ccggggacag
     5161 ggacgagagg ccgacgggac ggacgaagac ccgatcctgc cggggctcgc cctgtggaag
     5221 ggcgacgtga ggcacctcga caagggccag cacctgttca cgatccgcaa cgggcgccgg
     5281 ttcgcattca tgccgcagtt ctccgaggcc ccggcgcagg gcatcccgat cccgccgtac
     5341 ccgttgaccg agaagcagat cgaggccgtg ctcgagtacg agaggacgct gtaggtggac
     5401 agcatcttcg tcgagggcgt cgccgcgacc gtctcctcga tcatcgtgtt ctgcggaagc
     5461 gtgtggctcc tgctcgcgct cgtgctcggc ccccgtctct cctacttcat caccgcgtcg
     5521 gtcacgctcg ggttcctgct gatgatgggc gtcgtctggt cggtcggcga gccgctcggc
     5581 ccggtcggcg agctgccgtc gtactcgcag gtcggcgtcg gcgacacgcc cgccgagtcc
     5641 ggcttcggcc cggcaggcga cttccccgac ggaggtggct ggttcacgcc gaaccccgag
     5701 gaggccgccc acgccgagat caagagcggt gccgagggcg aggcccccga cctgctcgca
     5761 gaggcgatcg acgccggcga gatcacctcg ttcgagcagc cggaccaggc acaggtcgac
     5821 gccgactcga cgcagctgct cgagcaggac ggcacgttct acgcggcggt gcggttcgag
     5881 ccggtcccga gcgaccccga agaggaggac gccgcgagcg accaggacat cgcccccgag
     5941 gagggcgacg gccctgccga ggacgacgcc gcggcacagg aggaggagct ggacccgaac
     6001 accgaggcgt acgtcttcct cgagcaggac ccgggcgacc cctccggaca agcccggatg
     6061 atcacgctcg gcttcttcgt gctgctggtc ctgcacctga tgggcctgag cgccagcgag
     6121 cgccgcgcca agcgtgcgcg agcggagact tcctaggagg acatggtgcg aacccacatg
     6181 cggaccaccg ccacggcagg gctgctgctc ctcctgtccg ggtgcactcg caccggcatc
     6241 aacgccggcg agcagggggg ccaggccttc atcctcttca cggtcatgct gctcgtgacg
     6301 atcgcgatcc tctggttcat cctcggccgc gaggagtaga cctcccgctg cgcgcgttca
     6361 gcgccgtgaa gaacccgtag gccaggaaca cgtagccgac gaagaagatc cccacgcgca
     6421 accaggtcgg gacctcgtcg gccaccgtgt cgaggatcgc gccccccagc aggatcaacg
     6481 caccgatcag catcgtcacg cgccacttcg gccacttggt agcaggaggt ggcggactct
     6541 cgggagttcc cggccccgcc gggggcttaa cgggtggcga agacgccacg ggcggcctcc
     6601 acgagctggt ctacgtgctc ctccgtgtgg acggccgaca cgaacagtgc ctcgaagcgt
     6661 gacgggggca ggtagacgcc gtgcgccagc atccccctga agaagcgcgc gtacgtgccc
     6721 tggtcggtct tcttggcggc ttcgtagtcg agcacctcct cgcctgcgaa gaagaacccg
     6781 gccaggccgc cgatcgcggc ggcgaccatc gggacctcgg cctcgtccgc cgcgtcgacc
     6841 aatcccttga cgagcgaccg ggcgcgctcc tgaacccgtt cgtacaggcc ggggtcggac
     6901 tgcaccaggc ccagcgaggc gatccccgcc gccatcgcga gcgggttccc cgacagcgtt
     6961 cccgcctggt agacgggacc cacgggcgcg agctcgtcca tgacgtcgct ccggcccccg
     7021 atcgcggcca cgggaaggcc cgcgccgagg accttgccga gcatgacgag gtccgcccgg
     7081 accccgaagc gctcctgcgc gccgccgcgg ccgagccgga acccggtgat gacctcgtcg
     7141 aagacgagca gcgcgccggt ggcgtcggcg agcgcgcgca agccttgcag gaagccctcc
     7201 tcgggaagga cgaggcccat gttggcggcg acgggctcga cgaggatcgc ggcgaggtcg
     7261 tcctcgaagc cgtcgatcgc gtcgcggacg gattccaggt cgttgtactt ggcgacgagc
     7321 gtgtcgcccg cggctccctt ggtgacgccg ggggtccccg ggatcccgag cgtcgccact
     7381 ccggagcctg cgtcgaccag cagcgcgtcg acgtggccgt ggtagcagcc cgagaacttc
     7441 agcaccttgt cgcggccggt cgcgccgcgg gcgagccgta ccgcggtcat cccggcctcg
     7501 gtcccggacg acacgaagcg gaccttgtcc acgttcggta cgaggtcgac gaccagccgg
     7561 gccagctcga cctcgagctc gctcggcgtc ccgaacgacg tccccttgcg cgcggcctcg
     7621 gtgaccgcct cgacgatcgc gggatgcgcg tggccgaaca gcagcgcgcc ccagctctgg
     7681 acgaagtcga ggtaggtgtt gccgtcgacg tcggtgaggt aggggccgga cgcgctggcg
     7741 acgaagaacg gatgcgcgcc gacggcttcg aacgagcgaa cgggcgagtt gaccccgccg
     7801 ggcatcaggc tgaccgcctc ggcgagcagc ttgtccgacg cttctctcag aggctgcggc
     7861 ttcaactcag ccactcggcc gccttcttcg cgtggtacgt gaggatcaga tcggcgccgg
     7921 cccgcttgat ggaagtgagc gtctcgagcg tcgcggcccg ctcgtcgatc cagcctcgct
     7981 cggcggcggc catcagcatc gagtactcgc cgctcacgtt gtatgcggcg gtcgggaggc
     8041 cgaatcgttc cttcaccgcg cgcagcacgt cgaggtacgg gagcgccggc ttgaccatga
     8101 ccgcgtcggc gccttccgcg acgtcgagag cgacctcgcg caaagcctcg gcgacgttcg
     8161 cggggtcctg ctggtacgcg cggcggtcgc cgaactgtgg cgcaccctcg gcggcctcgc
     8221 ggaacggccc gtagaagctc gacgcgtact tggcggcgta ggccaggatc gggatctcct
     8281 ggtggccggc ggcgtcgagg gccgcgcgga tctcgccgac ctgcccgtcc atcatcccgg
     8341 acggcgcgac cacgtcggct cctgcggcgg cctgcgacgc ggcgatccgt ccgtagagcg
     8401 agatcgtgcg gtcgttgtcg accgtctcgc cagagaggat cccgcagtgg ccgtgcgacg
     8461 tgtactcgca caggcagagg tccgcgatca cgaccgcctc gtcgccgagc tcgtccttca
     8521 gcgccgacag cccccgctgc acgatcccgt cggggctcca cgcctcaaag ccggactcgt
     8581 ccttggccgc cgggatgccg aacaggatga ccccggcgac gccccggtcg gcgagctcgc
     8641 gcgcctcctt caccagggtc tcgagcgtgt gctggaagtg gccgggcatc gacgagatcg
     8701 ggaccgcgtc gtcgatgccc tccttgacga acagcggcgc gatcaggtcg gagggaaaga
     8761 cggtggtctc ggcgaacgcg cgccgcagcc cgggcgttcg ccgcagcctg cgcatccgtt
     8821 cggtggggaa gctcacggct ccaatggtgc cacgtggccg aaccgctcca cgagcgcacg
     8881 cacgaggccg gggacggagt gctcctcggc caccacgtct acgcgcagcc cggcctccgt
     8941 agccgccgtc gccgtgacgg ggccgatgca agcgaccgca cggttgcctc cgcctcctgt
     9001 ctcgaggcct agctcctggg gcgtccctgc gacctggagg aagttgcgga ccgtagacgc
     9061 gctcgtgaac gtcacgacgt cgacccgccg cgtcgcgacg gccgaggccc ccggcgtgtc
     9121 gcgatccgcc ggcaggttgc ggtacgccgc cacctcacgc acgctccaac cgttggacga
     9181 cagcgcgtcg acgacgtcgg ggggagcacc ttcgacacgc gg
//
LOCUS       DASQDO010000124         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1114034, whole genome shotgun sequence.
ACCESSION   DASQDO010000124 DASQDO010000000
VERSION     DASQDO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114034"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..917
                     /locus_tag="VEV43_03780"
     CDS             75..917
                     /locus_tag="VEV43_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYP22672.1"
                     /translation="MPATRTEDVTELTAIARRLRRDIVKATTAAASGHPSTSLSMVEI
                     LTVLYFGGVLRYDPADPQDPDRDRFILSKGHGAPGLYAALAEAGYFPREDLMTLRQMG
                     SPLEGHPNMCRLPGVEASTGSLGQGFSIAIGHALAARLDGGDYRVYVMVGDGESEQGQ
                     VWEAAMYAGNHGLDNLTLIVDRNGYQQTGAVDDIQPLDPLEDKLSAFGLEVRTIDGHS
                     LEEVAAAFEWARGVQGKPQAIVARTIKGKGVSLLEAKQGGFHGKPIPPDEEAKALQEI
                     GAGS"
     gene            914..1873
                     /locus_tag="VEV43_03785"
     CDS             914..1873
                     /locus_tag="VEV43_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYP22673.1"
                     /translation="MSFGLSRIDLPDGDPTREAFGRALADLGSDERIVVVDGDVNNST
                     FTNYFKERYPDRFFNAGIAESNMIGIAAGLSASGKIPVAASFSIFLLANAFDQIRMSV
                     GFPNQNVKLVGSHAGISIGEDGPSQMAIEDVALACAVPGMTVIVPADGPSTAAATKAM
                     IETRGPTFLRCGRPKVPVIYPDGAEYHLGKANTVREGDDITIIANGIMVAMALDAAAG
                     LADEGIEARVLDMHTVKPVDTAAIERAANETRGIVVAEEHMAYGGLGAVVSMVVSERS
                     PVRMAFVDVGDTYATSGKPDELLVQYGLTPDAIVDAARRVTKD"
     gene            1877..2107
                     /locus_tag="VEV43_03790"
     CDS             1877..2107
                     /locus_tag="VEV43_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22674.1"
                     /translation="MAGPIEFSATEAAIIFAVLGLAPPFVVAAIGSAVHARIAMHNGE
                     PVRPVMLFLKWWGLCTTAWLGAWILGALFSGG"
     gene            complement(2104..>2374)
                     /gene="dtd"
                     /locus_tag="VEV43_03795"
     CDS             complement(2104..>2374)
                     /gene="dtd"
                     /locus_tag="VEV43_03795"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HYP22675.1"
                     /translation="KMNLSLLDTGGAALVVSQFTLYGDARKGRRPSFIRAAQGPEAEA
                     LYEGVVAAFRALGVETGTGEFGAMMDVELVNDGPVTILLDSDKTF"
BASE COUNT          377 a          801 c          840 g          356 t
ORIGIN      
        1 aacgacgagg gaatccgctc gcgaaaacgc gcgtggtttc atgtgccggg agctgtccga
       61 gtcaaggagg ttcgatgccg gcgacgcgca ccgaggacgt cactgagctc acggccatag
      121 cccggcggct gcgccgggac atcgtcaagg caaccactgc ggctgcgtcg ggccacccct
      181 cgacgtcgct gtcgatggtc gagatcctca ccgtccttta cttcggcggc gtgctgcgct
      241 acgaccccgc ggatccacag gacccggacc gcgaccgctt catcctctcg aagggccacg
      301 gggcccccgg cctctacgcg gccctcgccg aggccgggta cttcccccgc gaggacctca
      361 tgaccttgcg tcagatgggc tcgcccctgg agggccaccc gaacatgtgc cggctccccg
      421 gcgtcgaggc gtcgacgggc tcgctcggcc agggcttctc gatcgcgatc gggcacgcgc
      481 tcgcggcacg gctcgacggc ggcgactacc gcgtctacgt gatggtcggc gacggcgaga
      541 gcgagcaggg ccaggtctgg gaggccgcga tgtacgccgg caaccacggc ctcgacaacc
      601 tcacgctgat cgtcgaccgc aacggctacc agcagacggg cgcggtcgac gacatccagc
      661 cgctcgaccc gctcgaggac aagctgagtg cgttcggtct cgaggtgagg acgatcgacg
      721 gccactcgct ggaggaggtc gccgcggcgt tcgagtgggc ccgcggcgtc cagggcaagc
      781 cgcaggcgat cgtcgcccgg acgatcaagg gcaagggcgt gtcgttgctc gaggcgaagc
      841 agggcggctt ccacggcaag ccgatcccac ccgacgagga ggcgaaggcg ctgcaggaga
      901 ttggagcggg ctcatgagct tcggactttc ccggatcgac ctccccgacg gcgacccgac
      961 gcgtgaggcg ttcgggcgcg cgctcgcgga cctcggctcc gacgagcgca tcgtcgtcgt
     1021 agacggcgac gtgaacaact cgacgttcac gaactacttc aaggagcgct acccggaccg
     1081 gttcttcaac gccgggatcg ccgagtccaa catgatcggg atcgccgcag ggctctcggc
     1141 ttcggggaag atccccgtcg ctgcgtcgtt ctcgatcttc ctgctcgcga acgcgttcga
     1201 ccagatccgc atgtcggtcg ggttcccgaa ccagaacgtc aagctcgtcg gctcgcacgc
     1261 gggtatctcg atcggcgagg acgggccgtc gcagatggcg atcgaggacg tggcgctcgc
     1321 ctgcgcggtg ccgggcatga ccgtgatcgt cccggccgac ggcccgagca cggccgcggc
     1381 gacgaaagcg atgatcgaga cccgcgggcc gacgttccta cgctgcggcc ggccgaaggt
     1441 gccggtcatc tatccggacg gcgcggagta ccacctcggc aaggcgaaca cggttcgcga
     1501 gggcgacgac atcacgatca tcgcgaacgg gatcatggtc gcgatggcgc tggacgcggc
     1561 cgcgggcctg gccgacgagg gcatagaggc acgggtgctc gacatgcaca cggtcaagcc
     1621 cgtcgacacc gcggccatcg agcgcgcggc gaacgagacg cgcgggatcg tcgtcgcgga
     1681 ggagcacatg gcgtacgggg ggctcggcgc ggtcgtctcg atggtcgtca gcgagcggag
     1741 ccccgtgcgg atggcgttcg tcgacgtcgg cgacacctac gcgacgtcgg gcaagccgga
     1801 cgagctgctg gtccagtacg gcctcacgcc cgacgcgatc gtcgacgcgg cccggagggt
     1861 gacgaaggac tagcccatgg cggggccgat cgagttctct gcgacagagg ccgcgatcat
     1921 ctttgcggta ctcggcctcg cccctccgtt cgtggtcgcg gctatcggct cggccgtgca
     1981 cgcgcgcata gcgatgcaca acggcgagcc ggtccggccc gtaatgctgt tcctgaagtg
     2041 gtgggggctc tgcacgacgg cctggttggg cgcgtggatc ctcggggccc tgttctccgg
     2101 cggctagaac gtcttgtcgg agtccagcag gatcgtcacg gggccgtcgt tcactagctc
     2161 tacgtccatc atggccccga actcgcccgt tcccgtctcg acgcccaggg cccggaaggc
     2221 tgcgacgact ccttcgtaga gggcttcggc ctcgggaccc tgcgcggccc ggatgaacga
     2281 cggccgtcgg cccttgcgcg cgtcgccgta gagcgtgaac tgcgagacga cgagggccgc
     2341 tccgccggtg tcgagcaggg acaggttcat cttg
//
LOCUS       DASQDO010000125         5276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_135606, whole genome shotgun sequence.
ACCESSION   DASQDO010000125 DASQDO010000000
VERSION     DASQDO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135606"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /gene="proB"
                     /locus_tag="VEV43_03800"
     CDS             complement(<1..471)
                     /gene="proB"
                     /locus_tag="VEV43_03800"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYP22676.1"
                     /translation="MSRPRVGDGDRLVVKVGTASLVDSSGVPDERRLKALCGGLARLH
                     RAGANPILVSSGAIAAGLGPLGLDRRPTDIPSLQAAAAVGQRLLLGSYAQLLAASDIV
                     AAQVLLTRYDFMHRQQYLNARNTIDRLLALKALPVVNENDTVAVDEIRFGDNDML"
     gene            complement(458..1768)
                     /gene="obgE"
                     /locus_tag="VEV43_03805"
     CDS             complement(458..1768)
                     /gene="obgE"
                     /locus_tag="VEV43_03805"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYP22677.1"
                     /translation="MAFVDEATIHVFGGAGGNGSASFHREPYKPKGGPDGGDGGRGGT
                     VILRADTSVGTLLELRDHPHVKATPGGHGMSKRRHGANGKDRVVLVPPGTVAYDEDGV
                     LVADLARAGDELVAAAGGRGGRGNVQFTTPTRRAPAFAEKGEPGEQRRLRLELRLLAD
                     VGLVGFPNAGKSTLISRVSAAKPKIADYPFTTLVPNLGVVPGEEAFVVADIPGLVPGA
                     SEGKGLGHRFLRHVSRAAILLFLVDPLAQDRDPASDVEVLAAELEAFDPDLAARPRLV
                     VVTKADAAPERAAEVAASHPGASVISAVSGEGMQELVERLRDLVREARAAAPARVGYV
                     RHVVKESPLEVAREDSAWRVTGRRAERAVATTDMDSEEAVELLQRRLIAMGVERMLAA
                     AGAVRGDEVRIGEVAFEFEPEGEAADRLGEPPGPEGSVDEGVET"
     gene            complement(1805..2056)
                     /gene="rpmA"
                     /locus_tag="VEV43_03810"
     CDS             complement(1805..2056)
                     /gene="rpmA"
                     /locus_tag="VEV43_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006898480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYP22678.1"
                     /translation="MAHKKGASSSRNGRDSEAKRLGVKMFGGQQAKPGMILVRQRGTR
                     LHPGLNVGKGSDDTLFAMAPGVVTFHRHRGRRVVSVIGE"
     gene            complement(2160..2450)
                     /gene="rplU"
                     /locus_tag="VEV43_03815"
                     /pseudo
     CDS             complement(2160..2450)
                     /gene="rplU"
                     /locus_tag="VEV43_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            complement(2507..4030)
                     /locus_tag="VEV43_03820"
     CDS             complement(2507..4030)
                     /locus_tag="VEV43_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYP22679.1"
                     /translation="MSDPSPRRREKATDLPSLEEVREAPQQREAPPQEAPREGERQSG
                     NRRRRRRGPKPDAPAQETSSAPEGDEAASKGSSSRRNRGRGRRKGPRKSFPRADRVML
                     VHAKPGGMQIGVLEDEQIVEHYVAREEDRSIVGNVYLGRVQNVLPGMEASFIDIGEAR
                     NGVLYAGEVGIAGDEGDEIPRIETVLKSGQSILVQVTKDPMRAKGARLTAIVSLAGRH
                     LVLVPNAHSTGVSRRLSDRERTRLRDIVAEIRPAEHGLIVRTAAEGAPREDLVRDLER
                     LVQTWEVISRKAQGSKAPALLYEEPELEMRVIRDLFNRDVSRCVVDDPGLAGKLRDYV
                     RSSSSELGDRIEVYEGSLPIFEEYRVVEQIRKSLDRKVWLPSGGHLVVDRTEAMTVID
                     VNTGKFVGKSNLEETVFRTNKEAAVEVGRQLRLRDIGGIIVIDFIDMEDLENRDEVIK
                     VFREELGRDRTRTQVFDISPLGLVQMTRKNVSAGIVEAFSDPCPTCEGRGILIHDVD"
     gene            complement(4027..5205)
                     /gene="rodA"
                     /locus_tag="VEV43_03825"
     CDS             complement(4027..5205)
                     /gene="rodA"
                     /locus_tag="VEV43_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HYP22680.1"
                     /translation="MAMEGIFGEAVDRIGGEPISARMARKAPLRHLDATLLLVTLLLT
                     GFGAVMILSATQHKQEAAGLDPTIFLDRQLAFIVAATVVLFAVSFFDYRHFRAFAPVM
                     YVGVLLALVAVATPLGQEIQGSQRWIDLGFFQMQPSEMAKVVVIVTLAAYFSMLKGQL
                     RARDVAAAVGLVALPCLLIYVQPDLGTMMVFVALLGVMLLVGGAKIRHLLVLAMLGVG
                     LVGFVVQAGLLEDYQIQRITAFLDPSPDVQSEGYNLAQSEIAIGSGGISGKGLEGKNT
                     QTSLDFIPEQHTDFIFTAVGEQLGFVGSATLLSLFAFLLWRALRIAAMSRDLFGTLIA
                     AGIAGYWGFQLFINLGMTMGIMPITGIPLPFISYGGSSLITNYMAVGLLLNVHMRRFL
                     "
BASE COUNT          757 a         1866 c         1841 g          812 t
ORIGIN      
        1 gagcatgtcg ttgtcgccga agcggatctc gtcgaccgca accgtgtcgt tctcgttgac
       61 gaccgggagc gccttgagcg cgagcaggcg gtcgatcgtg ttgcgcgcgt tcaggtactg
      121 ctggcggtgc atgaagtcgt agcgcgtgag gagcacctgc gccgcgacga tgtccgacgc
      181 cgcgaggagc tgcgcgtagc tgccgagcag caggcgctga ccgacggccg ccgcggcctg
      241 gagcgacggg atgtcggtcg ggcggcggtc gaggccgagg gggccgaggc cggccgcgat
      301 cgcgccggac gagacgagga tcggattcgc gcccgcgcga tgaaggcgcg ccaacccgcc
      361 gcagagcgcc ttcagccggc gctcgtcggg caccccgctc gagtcgacga gcgatgccgt
      421 gccgaccttc acgacgagcc ggtcgccgtc accgacccta ggtctcgaca ccctcgtcca
      481 ccgagccctc cggcccgggc ggctcgccga ggcgatccgc cgcctcgccc tcgggctcga
      541 actcgaacgc gacctcgccg atgcggacct catcaccgcg cacggcgccg gccgcggcga
      601 gcatgcgctc gacccccatc gcgatcagac gccgctgcag cagctcgacc gcctcctcgc
      661 tgtccatgtc ggtggtcgcg acggcgcgct cggcgcggcg gccggtgacg cgccaggccg
      721 agtcctcccg cgcgacctcg agcgggctct ccttgacgac gtgccggacg tagccgaccc
      781 gggcgggggc cgcggcgcgt gcctcgcgca cgaggtcacg cagccgctcg accagctctt
      841 gcatgccctc gccgctcacg gccgagatca cggacgctcc gggatgggac gcggccacct
      901 cggccgcgcg ctcgggggcc gcgtcggcct tcgtcacgac gacgaggcgc gggcgcgcgg
      961 cgaggtcggg gtcgaaggcc tcgagctccg cggcgagcac ctcgacgtcc gaggccggat
     1021 cgcggtcctg cgccagcggg tcgacgagga acagaaggat cgcggcgcgg ctcacgtggc
     1081 gcaggaacct gtggccgagg cccttgccct cgctcgcacc ggggacgagc ccggggatgt
     1141 ccgcgacgac gaaggcctct tcaccgggga cgacgccgag gttcggcacg agcgtggtga
     1201 acgggtagtc ggcgatcttg ggcttcgccg cggagacccg gctgatcagc gtggacttgc
     1261 cggcgttcgg gaagccgacg aggccgacgt ccgccagcag acgcagctcg agacggaggc
     1321 ggcgctgctc gcccggctcc cctttctccg cgaacgcggg agcgcgccgg gtcggcgtcg
     1381 tgaactggac gttcccgcgg ccccctcgcc cgccggccgc ggccacgagc tcgtcgccgg
     1441 cccgcgcgag gtccgccacg aggacgccgt cctcgtcgta cgcgaccgtc ccgggaggca
     1501 cgagcacgac gcggtccttg ccgttcgcgc cgtggcggcg tttgctcatg ccgtgcccgc
     1561 cgggcgtcgc cttgacgtgc ggatggtcgc gcagctcgag cagcgtcccg accgacgtgt
     1621 ccgcgcgcag gatcacggtg ccgccgcggc ccccgtcgcc gccgtcgggc ccgcccttgg
     1681 gcttgtaggg ctcgcggtgg aacgacgcgg agccgttgcc gccggcaccg ccgaagacgt
     1741 ggatcgtggc ctcgtctacg aaagccatcg cgtgatgagg gaagcccgcg tcgcgggcga
     1801 ctcgctactc gccgatgacg ctgacgacgc gccggccacg gtgacggtgg aacgtcacga
     1861 caccgggcgc cattgcgaac agcgtgtcgt cgctgccctt gccgacgttg aggccggggt
     1921 ggaggcgcgt cccgcgctgg cgcacgagga tcatcccggg cttcgcctgc tggccgccga
     1981 acatcttgac gccgagccgc ttcgcctcgg agtcgcgccc gttacgggaa gacgatgctc
     2041 ccttcttatg cgccatcggt cccctcctcg gcgggtgcgg ccgccttctt ctcggtttcc
     2101 ttcgtgcgtc ccgccgaggc ggtccgcgcg gcggcgccac cgctcagggt gatgtcgccg
     2161 atctcgatcg tcgtgtactt ctgccggtgt ccgcggtgcc tgcggtagcc ggtcttgttc
     2221 ttgaacttgt tgacctcgac cttggggccc ttggcctcgc cgacgacctt gccgacgacg
     2281 cgggcgctcg agaggtcgcc cttgtcggag cgcgtcgtgc cggcgtcgtc gacgacgagg
     2341 agtggcgtga actcgacgga gtccgagccc tccttcacgc gctcgatctc gattacgtcg
     2401 ccgggggcaa ccttgtgctg cttaccgccc gcgcggatga tggcgtacaa gtcattctcc
     2461 ttggatttgg aagccgacag aggataccaa atgtcccggc ccacccctag tcgacgtcgt
     2521 ggatgaggat cccgcggccc tcacaggtcg gacaggggtc cgagaacgcc tcgacgatcc
     2581 cggccgagac gttcttgcgg gtcatctgga cgagccccag cggggagatg tcgaagacct
     2641 gcgtccgcgt ccggtcgcgg cccagctcct cccggaagac cttgatgacc tcgtcgcggt
     2701 tctcgaggtc ctccatgtcg atgaagtcga tgacgatgat cccgccgatg tcgcggagcc
     2761 ggagctggcg cccgacctcg acggccgcct ccttgttcgt gcggaacacg gtctcctcga
     2821 ggttcgactt cccgacgaac ttcccggtgt tgacgtcgat caccgtcatc gcctcggtgc
     2881 ggtcgacgac gaggtgaccg ccggacggta gccagacctt gcggtcgagc gacttgcgga
     2941 tctgctcgac gaccctgtac tcctcgaaga tcggcagcga gccctcgtag acctcgatgc
     3001 ggtcgccgag ctcggacgag ctcgaccgga cgtagtcgcg gagcttgccg gccaggccgg
     3061 ggtcgtcgac gacgcagcgg ctcacgtcgc ggttgaagag gtcgcggatc acgcgcatct
     3121 cgagctcggg ctcctcgtag agcagcgccg gcgccttcga gccctgcgcc ttccggctga
     3181 tgacctccca cgtctggacg agcctctcga ggtcgcgcac gaggtcctcg cgcggtgcgc
     3241 cttcggccgc ggtgcgcacg atcagcccgt gctccgcagg acggatctcg gcgacgatgt
     3301 cgcggagccg cgtcctctcg cggtcggaga gccggcgcga cacccccgtc gagtgcgcgt
     3361 tcgggaccag cacgaggtgc cgccctgcga gcgagacgat cgccgtgagg cgcgcgccct
     3421 tggcgcgcat cgggtccttc gtgacctgca cgaggatcga ttggccgctc ttgagcacgg
     3481 tctcgatgcg cgggatctcg tcgccctcgt ccccggcgat cccgacctcg cccgcgtaga
     3541 ggacgccgtt gcgggcctcg ccgatgtcga tgaacgaggc ctccattccg ggcagcacgt
     3601 tctgcacgcg gccgaggtag acgttgccga cgatcgaccg gtcctcttcg cgggcgacgt
     3661 agtgctcgac gatctgctcg tcctcgagca cgccgatctg catgccgccc ggcttcgcgt
     3721 gcacgagcat cacgcggtcg gcgcgcggga acgacttgcg cgggcccttg cggcggccgc
     3781 ggccccggtt gcgtcgcgag gacgacccct tggacgcggc ttcgtcgccc tccggcgcgg
     3841 acgaggtctc ctgcgccggg gcgtccggct tcggcccccg gcgacggcga cgccggttgc
     3901 cgctctgccg ttctccttct cgtggcgctt cttgcggtgg tgcttcgcgt tgctgtggtg
     3961 cctcccgcac ctcttcgagg ctcgggaggt ccgtcgcctt ctcgcggcgg cggggagacg
     4021 ggtcgctcat aggaaccgcc tcatgtggac gttgagcagc aggcctacgg ccatgtagtt
     4081 ggtgatcagg ctggaacctc cgtaagagat gaagggaagg gggatgccgg tgatcggcat
     4141 gatccccatg gtcataccca ggttgatgaa cagctggaac ccccaatacc ccgcgatgcc
     4201 cgcggctatc agggtgccaa agaggtcgcg cgacatcgcg gcgatgcgca acgccctcca
     4261 cagcaggaag gcgaacaggg acagcagggt cgccgagccg acgaagccga gctgctcgcc
     4321 caccgcggtg aagatgaagt cggtgtgctg ctcggggatg aagtcgagcg aggtctgcgt
     4381 gttcttgccc tcgagcccct tgccgctgat gccgccggag ccgatcgcga tctcggactg
     4441 ggcgaggttg tagccctcgg actgcacgtc ggggctcggg tcgaggaacg ccgtgatccg
     4501 ctggatctga tagtcctcga gcaagccggc ctgcacgacg aacccgacga gccccacccc
     4561 gagcatcgcg aggacgagca ggtggcggat cttcgccccg ccgacgagca gcatcacccc
     4621 gagcagcgcg acgaacacca tcatcgtccc gaggtccggc tgcacgtaga tcagaagaca
     4681 cggcagcgcg acgagcccga ccgccgccgc cacgtcgcgc gcccgcagct gtcccttgag
     4741 catcgagaag tacgcggcga gcgtgacgat cacgacgacc ttcgccatct ccgacggctg
     4801 catctggaag aagccgaggt cgatccagcg ctgagaaccc tggatctcct gtcccagcgg
     4861 cgtcgcgacc gcgacgagcg ccaggagcac gccgacgtac atgaccggcg cgaacgcgcg
     4921 gaagtgccgg tagtcgaaga agctgaccgc gaacagcacg acggtcgcgg cgacgatgaa
     4981 cgcgagctgg cggtcgagga agatcgtcgg gtccagcccc gccgcctcct gcttgtgctg
     5041 cgtcgccgac aggatcatca ccgcgccgaa acccgtcagc agcagcgtca cgagcaacag
     5101 cgtcgcgtcg aggtggcgta gcggagcctt gcgcgccatg cgcgcggaga tgggctcgcc
     5161 gcctatgcgg tcgaccgcct cgccgaagat cccctccatc gccatcagcc ggactcgtcc
     5221 tggccgaggg tgaccccggt ctcctggtcg atgccgaaga ggccctcgaa cacctc
//
LOCUS       DASQDO010000126         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_563642, whole genome shotgun sequence.
ACCESSION   DASQDO010000126 DASQDO010000000
VERSION     DASQDO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563642"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1496)
                     /locus_tag="VEV43_03830"
     CDS             complement(90..1496)
                     /locus_tag="VEV43_03830"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HYP22681.1"
                     /translation="MPDDKRGPLPTGWAQRGAKLAGQTGKSALRFLGTRARSFAAPER
                     SQEFLDGFHQKTAEQLVQMLGEMKGAAMKVGQLASFYEFSAPGEYLATYKDALTMLQN
                     SAPPMDPEASKAVIKEEFGKGPDELFAEFGDEAIAAASIGQVHRARLHSGEEVAVKVQ
                     YPGVDEAVRSDLKNISAMTKLSVAIAPNVDPKEVANEVRDRVMEELDYRREAANQAKF
                     AELFAGHPYIVVPQVYPEYCRTRVIVQEFVEGEPFMSAFEWDQEDRDRLAEVLFRFFY
                     GSFNRFLLFSADPHPGNYLLLPDGKVAFLDYGLVRALDPGTLTVLIEIVLSLIDEDRE
                     RGRAALEAVGILNPKTPEVDAVWEHLKMLNAPVLEDREFRITAQMVQEIAAAGFDPRS
                     KAFQTLRKVGVPGVVITVNRMSFGVASLLGRLEATNNWQAIMREMWFGEESSTDMGRE
                     EQAWLAETHPDLTPPLAD"
     gene            1515..2417
                     /locus_tag="VEV43_03835"
     CDS             1515..2417
                     /locus_tag="VEV43_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYP22682.1"
                     /translation="MTGRWVSLLIAALTLLTAAPASPALLGDSAAQTQEIGPFTKDGA
                     MPHVVKLARHVGVRVRGTTGERKAARYVAAEFRSMGYSVNVQKYPVDGRTSRNVVAWW
                     PGARRYPVVIGAHMDTVAGSPGANDNASGVAVVLEVARIVAGTPKAEFVRFVAFGSEE
                     YGTNGLHHVGSQVFVDRLKEKGRDRLAGMISVDMIADGRPLIVATAGIGPPKIADRFA
                     AIARARDIGVDRQTTCDCSDNGPFERAGITAAFRWSGSEPNYHDDSDKVKNMKPDDLV
                     RTGRAVRYFVKQLDAGMLRRLRRA"
     gene            2497..3171
                     /locus_tag="VEV43_03840"
     CDS             2497..3171
                     /locus_tag="VEV43_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HYP22683.1"
                     /translation="MATGTVTVRGDALVPGEPDEAHVFLEIKAVEKSPQKAMADASAR
                     SDRLRGIFEELGIPAVARATSGISIGEEVEWSSGKRKHLGYHAVNRVSVRLDDVEKVA
                     RLMAEATEKCEATVRGPYWQIALANPARVEAFRTAAEDARRKAAAYASALGTSLGDVL
                     SVIEPGLAVRTHQRHEFVPPPVPAPVQAAAAPGRAAPPAPDIQVDAGALDIPASVEVT
                     FALEQS"
     gene            3168..3875
                     /locus_tag="VEV43_03845"
     CDS             3168..3875
                     /locus_tag="VEV43_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYP22684.1"
                     /translation="MSQAGGGGIGGPGGGVEGGGGSRYDEHPDEPQPQTFSCPKCGHS
                     LTVGTPICGNCGTILQGGSYVPEPLAAHGFRPGPIISIALTAALGIAGFLGRDSIGDF
                     FSRTVDDVSVDAPNFPDIDIPDIDPGDPLGPRVPGARNIGQIVREIRAAGIPCTQMDV
                     QSADDYVATGSCQSEGRHVQINIYFRQVTFDAGRDFFISDWPFATAQRDNWWISGDTA
                     VVRAIARGLDAKFRPPS"
     gene            3921..>4615
                     /locus_tag="VEV43_03850"
     CDS             3921..>4615
                     /locus_tag="VEV43_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HYP22685.1"
                     /translation="MSIAAPGRTPLEVVRGSLAVGVTLAGVSAGLTLLYLGMRAIMDV
                     GGACASGGPYVPRVECPEGVPLLMVAGIWGGIISVGLYLWATSRHKAPTLVAFAWPAL
                     FLSLGWNFLDYGIDDPAGGGLVWGWLICGILFAIMGGLPLPAIVKPVLDQFGRKAPER
                     PQPKVVIPTKKNVKKLREAHQPRPPKSQQFGFDPKPGPAGAAAGSFVDELERLRTLHQ
                     SGALSDREFAQAK"
BASE COUNT          684 a         1621 c         1614 g          696 t
ORIGIN      
        1 cgagattcgg cgcgacggtc tcccaccccg caaggttact cattcgtaac tctgacgaag
       61 tcgacgtgac ctccgaagcg cccggaagct cagtcggcga gcgggggcgt caggtcgggg
      121 tgggtctcgg ccagccaggc ctgctcctca cggcccatgt ccgtcgacga ctcctcgccg
      181 aaccacatct cgcgcatgat cgcctgccag ttgttcgtcg cctccagccg gcccagcaac
      241 gacgcgaccc cgaacgacat ccggttcacc gtgatgacga cgccgggcac gcccaccttc
      301 cgcagggtct ggaacgcctt gctgcgcggg tcgaacccgg cggcggcgat ctcctggacc
      361 atctgcgcgg tgatccggaa ctcgcggtcc tcgagcaccg gtgcgttgag catcttcagg
      421 tgctcccaca ccgcgtcgac ctcgggcgtc ttcgggttca ggatgccgac ggcctccagc
      481 gcggcgcggc cccgctcgcg gtcctcgtcg atcagcgaca gcacgatctc gatcaggacc
      541 gtcaacgtgc cggggtcgag cgcacgcacg aggccgtagt cgaggaacgc gaccttgccg
      601 tccgggagca gcaggtagtt gccggggtga gggtcggccg agaacagcag gaaccggttg
      661 aagcttccgt agaagaaacg gaacaggacc tcggcgagac ggtcgcgatc ctcctggtcc
      721 cactcgaacg cgctcatgaa cggctcgccc tcgacgaact cctggacgat cacgcgcgtc
      781 cggcagtact ccgggtagac ctgcggcacg acgatgtacg gatggccggc gaagagctcg
      841 gcgaacttgg cctggttcgc ggcctcgcgg cggtagtcca gctcttccat cacgcgatcg
      901 cggacctcgt tcgcgacctc cttcgggtct acgttcggcg cgatcgcaac cgagagcttc
      961 gtcatcgccg agatgttctt gaggtccgag cggacggcct cgtcgacgcc ggggtactgg
     1021 accttgaccg cgacctcttc gccggaatgc agccgcgcgc ggtgcacctg tccgatcgac
     1081 gcggcggcga tcgcttcgtc gccgaactcc gcgaacagct cgtcggggcc cttgccgaac
     1141 tcctccttga tcacggcctt cgacgcctca gggtccatcg ggggagccga gttctgcagc
     1201 atcgtcagcg cgtccttgta cgtcgcgagg tactcgccgg gggccgagaa ctcgtagaac
     1261 gacgcgagct ggccgacctt catggcggcc cccttcatct cgccgagcat ctggacgagc
     1321 tgctccgcgg tcttctgatg gaagccgtcg aggaactcct gcgagcgctc cggcgcggcg
     1381 aacgatctgg ccctcgtccc gaggaatcgg agcgcggact tgccggtctg gccggcgagc
     1441 ttcgcccccc gctgcgccca ccccgtaggg agcggtcccc tcttgtcgtc gggcatgagg
     1501 gctactctag ttgcgtgacc gggagatggg tttccctgct gatcgcggcc ctgaccctgc
     1561 tgacggcggc gccggcgtct ccggccctgc tgggcgactc ggccgcgcag acccaggaga
     1621 tcggcccctt cacgaaggac ggcgcgatgc cgcacgtcgt gaagctcgcc cgtcacgtcg
     1681 gcgtgcgagt ccgagggacg accggcgagc gcaaggcggc ccgttacgtc gccgccgagt
     1741 tccgctcgat gggctactcg gtcaacgtcc agaagtaccc ggtcgacggg cgcacgtcgc
     1801 gcaacgtcgt cgcgtggtgg ccgggcgccc ggcggtaccc cgtcgtcatc ggcgcgcaca
     1861 tggacacggt cgccggatcg ccgggcgcga acgacaacgc gtcgggtgtc gcggtcgtgc
     1921 tcgaggtcgc gcggatcgtc gcggggacgc cgaaggcaga gttcgttcgg ttcgtcgcgt
     1981 tcggctccga ggaatacggg acgaacgggc tccaccacgt cggctcgcag gtcttcgtgg
     2041 accggctgaa ggagaagggc cgcgacaggc tcgcagggat gatctcggtc gacatgatcg
     2101 ccgacgggcg gccgctgatc gtcgcgaccg cgggcatcgg cccgccgaag atcgccgacc
     2161 gcttcgcggc gatcgcccgt gcgcgcgaca tcggcgtcga tcgccagacg acctgtgact
     2221 gctccgacaa cggtccgttc gagcgggccg ggatcacggc ggcgttccgg tggagcggct
     2281 ccgagccgaa ctaccacgac gactcggaca aggtgaagaa catgaagccg gacgacctcg
     2341 tccgcacggg ccgggcggtc cggtacttcg tgaagcagct ggacgcaggg atgctgcgcc
     2401 gcctgcgcag ggcctagccg ggaggtagcc tctagcaagg cgaggacttc tcggcgcggc
     2461 ggcctcgggc aatgcgcggg aaagaaggga gcggctgtgg cgactgggac cgtgacggtg
     2521 cggggcgatg ccctcgttcc gggcgagccg gacgaggctc acgtcttcct cgagatcaag
     2581 gccgtcgaga agtcgccgca gaaggcgatg gcggacgcgt cggcgcgcag cgaccggctc
     2641 cgcggcatct tcgaggagct cggcataccg gccgtcgccc gggcgacctc cggcatctcg
     2701 atcggcgagg aggtcgagtg gtcctcgggg aagcgcaagc acctcggcta ccacgccgtg
     2761 aacagggtct cggtccgcct cgacgacgtc gagaaggtcg cccgcctcat ggcggaggcc
     2821 acggagaaat gcgaggcgac ggtgcggggg ccctactggc agatcgcgct cgcgaacccg
     2881 gcccgggtcg aagcgttccg gaccgccgcc gaggacgccc ggcggaaagc cgcggcctac
     2941 gcgtccgctc tcggcacctc gctcggtgac gtgctctcgg tgatcgagcc cgggctcgcg
     3001 gtgcgcaccc accagcggca cgagttcgtg cctcccccgg tcccggcccc ggtccaggcc
     3061 gccgccgcgc cggggcgagc cgccccgccg gcaccggaca tccaggtcga cgccggggca
     3121 ctcgacatcc ccgcctccgt cgaggtcacg ttcgcattgg agcagtcgtg agccaggcgg
     3181 ggggcggagg catcggcggg ccgggcggcg gcgtggaggg cggcgggggg agccgatacg
     3241 acgagcaccc ggacgagccg cagccccaga cgttctcgtg ccccaagtgc gggcacagcc
     3301 tgacggtcgg gactcccatc tgcggtaact gcgggacgat cctgcagggc gggtcgtacg
     3361 tccccgagcc cctggccgcg cacggcttcc gtcccggccc gatcatctcg atcgcgctga
     3421 cggccgcgct ggggatcgcg gggttcctcg gacgcgactc gatcggcgac ttcttcagcc
     3481 ggacggtcga cgacgtgtcc gtcgacgcgc cgaacttccc tgacatagac atccccgaca
     3541 tcgacccggg cgacccgctc ggcccccgcg tcccgggcgc ccgcaacatc ggtcagatcg
     3601 tccgcgagat cagggccgcg gggatcccct gcacccagat ggacgtccag tccgccgacg
     3661 actacgtcgc gaccgggagc tgccagtccg agggccgcca cgtccagatc aacatctact
     3721 tcaggcaggt gacgttcgac gcggggcgag acttcttcat cagcgactgg ccgttcgcga
     3781 ccgcgcagcg ggacaactgg tggatctcgg gcgacacggc cgtagtgcgt gcgatcgccc
     3841 gcgggctcga cgctaagttc cggcccccct cgtaaccgaa ggtccttcct caccgtcgcc
     3901 ggggccgcta ccttgacgac gtgtcgatag ccgcccccgg tcgcactccg ctcgaggtcg
     3961 tgcgcgggtc tctcgccgtc ggcgtgacgc ttgcaggcgt ctcggccggt ctgacgctgc
     4021 tctacctcgg catgcgcgcg atcatggacg tcggcggggc gtgcgcgtcg ggcgggccgt
     4081 acgtgccgcg ggtcgagtgc ccggagggcg tcccgctcct gatggtcgcg ggcatctggg
     4141 gagggatcat ctccgtcggc ctctatctct gggcgacgtc caggcacaag gccccgacgc
     4201 tggtcgcgtt cgcgtggccc gcgctgttcc tgtcgctcgg gtggaacttc ctcgactacg
     4261 gcatcgacga cccggcgggc ggcgggctcg tctggggatg gctgatctgc ggcatcctgt
     4321 tcgcgatcat gggcggcctc ccgctgccgg ccatcgtcaa gccggtcctg gaccagttcg
     4381 gccggaaggc gcccgagagg ccgcagccga aggtcgtcat cccgacgaag aagaacgtga
     4441 agaagctgcg cgaggcgcac cagcctcggc ccccgaagtc ccagcagttc ggcttcgacc
     4501 cgaagccggg gccggccggc gccgccgcgg gctcgttcgt cgacgagctc gaacggctcc
     4561 gcacgctgca ccagtccggc gccctgtccg accgtgagtt cgcccaggcc aagaa
//
LOCUS       DASQDO010000127         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_869686, whole genome shotgun sequence.
ACCESSION   DASQDO010000127 DASQDO010000000
VERSION     DASQDO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869686"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VEV43_03855"
     CDS             complement(<1..720)
                     /locus_tag="VEV43_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYP22686.1"
                     /translation="MTNIMLAGLVGLVVSLFFTPLAVKRFRRRGWGQLIREEGPKAHY
                     EKRGTPTMGGLVILGGTIAGYVLGHFGSGGFSPFEDSGLLAIGTIAALAGLGFLDDFI
                     KIRKSRSLGLQKRAKFLGQLIIAAVFGVLAVEVVGIGTDLSFFRSTALDLEVFFYVWV
                     FLMIAAASNGVNLTDGLDGLAVGSAAQVLAAFVVIGFWQFRHQIFYDIGETGSDPFEL
                     AIVAAAMFGACAGFLWWNTAPA"
     gene            complement(717..>1673)
                     /locus_tag="VEV43_03860"
     CDS             complement(717..>1673)
                     /locus_tag="VEV43_03860"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HYP22687.1"
                     /translation="DSVAMEVTSHALALHRVEGLCFAAAAFTNLTQDHLDFHDSMEDY
                     FEAKRSLFVAGRLERGAANVDEVYGRRLMEVAEVPCVGFGLAPEAGVRAVDVELGPAG
                     SKFRIVTPVGEIAVESSLAGSFNVSNCLAAAATCLQAGIGLGAVEAGLNSGTSVPGRF
                     ESVDAGQDFSVVVDYAHTPDSLDNVLRAARGLAERSGGRVIALFGCGGDRDRGKRPLM
                     GAVAARLADFVVVTSDNPRSEEPQAIVDEILEGVMAERADGPDAVDVDRRAAIATAVR
                     EAKPGDVVVIAGKGHETGQQFRDHTIPFDDRIVAREILEQLA"
BASE COUNT          281 a          599 c          573 g          220 t
ORIGIN      
        1 ggccggggcc gtgttccacc acaagaagcc cgcgcacgcg ccgaacatcg cggctgcgac
       61 gatcgcgagc tcgaaggggt cggaccccgt ctcgccgatg tcgtagaaga tctggtgccg
      121 gaactgccag aacccgatga cgacgaacgc ggcgagcacc tgcgccgcgg acccgaccgc
      181 gagaccgtcg agaccgtccg tcaggttcac gccgttcgag gccgcggcga tcatcaagaa
      241 gacccagacg tagaagaaga cctcgaggtc gagcgcagtc gagcggaaga acgagaggtc
      301 ggtgccgata cccacgacct cgacggcgag cacgccgaag accgcggcga tgatcagctg
      361 cccgaggaac ttcgcccgct tctgcagccc gagcgaacgc gacttgcgga tcttgatgaa
      421 gtcatcgagg aagccgaggc ccgcgagggc cgcgatcgtc cctatcgcca gcaacccgga
      481 gtcctcgaac ggagagaacc cgccgctccc gaagtggccg aggacgtacc cggcgatcgt
      541 cccgccgagg atcacgagcc cgcccatcgt cggcgtgccg cgcttctcgt agtgggcctt
      601 cggcccctcc tcgcggatca gctggcccca gccgcgccgg cggaagcgct tcacggcgag
      661 cggggtgaag aacaacgaca cgacgaggcc gacgagcccg gcgagcatga tgtttgtcac
      721 gcgagttgct ccaggatctc gcgtgcgacg atccggtcgt cgaacgggat cgtgtggtcg
      781 cggaactgct gcccggtctc gtggcctttc cctgcgatca cgacgacgtc gcccggcttg
      841 gcctcgcgca ccgcggtcgc tatcgccgcg cggcggtcga cgtccacggc gtcggggccg
      901 tccgcccgct ccgccatgac gccctcgagg atctcgtcga cgatcgcctg aggctcctcg
      961 ctgcgcgggt tgtcggacgt gacgacgacg aagtcggcga gccgcgccgc gaccgcgccc
     1021 atcaacgggc gcttcccgcg gtccctgtcc ccaccgcagc cgaacagggc gatcacgcgg
     1081 cccccgctcc tctcggcgag cccccgcgcc gcgcgcagga cgttgtccaa ggagtccggc
     1141 gtgtgcgcgt agtcgacgac gaccgagaag tcctgacccg cgtcgacgga ctcgaagcga
     1201 cccgggacgg acgtccccga gttgagaccg gcttcgacgg cgccgagccc gatcccggcc
     1261 tggaggcacg tcgccgcggc cgcgaggcag ttcgagacgt tgaacgagcc cgcgagcgac
     1321 gactcgacgg cgatctcgcc gaccggcgtg acgatgcgga acttgctgcc ggcgggcccg
     1381 agctcgacgt cgacggcacg caccccggct tcgggcgcga ggccgaagcc gacgcacggc
     1441 acctctgcga cctccatgag gcgccggccg tacacctcgt cgacgttggc ggccccgcgc
     1501 tcgaggcgcc ccgcgacgaa cagcgaccgc ttggcctcga aatagtcctc catcgagtcg
     1561 tggaagtcga ggtggtcttg ggtgaggttc gtgaacgctg cggccgcgaa gcagagaccc
     1621 tcgacgcggt ggagcgcgag cgcgtggctc gtcacctcca tcgcgacgga gtc
//
LOCUS       DASQDO010000128         5932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_930703, whole genome shotgun sequence.
ACCESSION   DASQDO010000128 DASQDO010000000
VERSION     DASQDO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930703"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEV43_03865"
     CDS             <1..295
                     /locus_tag="VEV43_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012903739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYP22688.1"
                     /translation="LKEEGIDTLILGCTHYPLLSGMIRYVMGEEVVLVSSADATAADV
                     YAKLRDDDLLKDTVDLGEHRFIVSSPEGISSELGRRFLGPEFRNAEHRPWGDR"
     gene            292..1035
                     /locus_tag="VEV43_03870"
     CDS             292..1035
                     /locus_tag="VEV43_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP22689.1"
                     /translation="MTTRITILGSSGRFATAHRASSGYLLEIDGHRLWLDAGAGTWRN
                     LLGQCAFDEVDGVILTHRHPDHVTDVYQLQHALLYGPKGRLPEIPLWAPAQTIESMSA
                     YDDLHDAFEPHVVAGGDSIEVAGATIRFVSMAHPPETVGVRVETADGVLAYSADSGEE
                     ADFEALAGGAGLFVCEASNQDSDDIWEGHLRASQAARIATGAGVERLVLTHLPVERDL
                     GLSLAEAQAAVDGPSVQLAADGDSHEVGR"
     gene            1032..1763
                     /gene="rph"
                     /locus_tag="VEV43_03875"
     CDS             1032..1763
                     /gene="rph"
                     /locus_tag="VEV43_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYP22690.1"
                     /translation="MTRADGRAPGELRPVAMTLGFMPYAEGSCLVEMGETKVICTASV
                     EGSVPRWMQGQGKGWVTAEYGMLPRATTTRRRRDVEKGRPDGRTQEIQRLIGRSLRAV
                     VDMDALGERTVWVDCDVLRADGGTRTASITGAFVALAEAFRGLEEKELVSKSPLLDSV
                     SAVSVGIVGGTPCLDLNYVEDSTAQVDMNVVMTGAGKLVEVQGTAEQGAFERSELDRL
                     LDLATDGIAQLTAHQQRALGAPAAE"
     gene            1760..2338
                     /gene="rdgB"
                     /locus_tag="VEV43_03880"
     CDS             1760..2338
                     /gene="rdgB"
                     /locus_tag="VEV43_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HYP22691.1"
                     /translation="MKAVLASRNRHKAEQVALLLPDLELVPLDDVAPDLELAEPYDTF
                     EDNALAKARAVVAATGLPAVADDSGLEVDALGGAPGVFSARYAGDDATDEANNEKLVD
                     ALAGTPEDDRSCRYRCVAVFVAPDGRELVASGSCEGTVVLEGRGTLGFGYDPHVIPEC
                     ATRTMGEIPLDEKLRFSHRGRAFRALAAMLGR"
     gene            2335..2994
                     /gene="pdxH"
                     /locus_tag="VEV43_03885"
     CDS             2335..2994
                     /gene="pdxH"
                     /locus_tag="VEV43_03885"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HYP22692.1"
                     /translation="MSDLERVSESIAHLRRDHVVQPLEIEDLDPDPIVQFGKWLEEAL
                     AEGLMLPNTMTLATATPEGRPSARMVLLKGFDHDGFVFYTNYESRKARELSANPHAAL
                     VFYWARLERQVRVEGRVERVPAEESDEYFASRPLESRLGAWASRQSAPLASRAELEER
                     VARLEQQYTSGEVPRPPHWGGWLVRPESIELWQGRPNRLHDRFLYSCSDDGWERVRLY
                     P"
     gene            complement(2985..3755)
                     /locus_tag="VEV43_03890"
     CDS             complement(2985..3755)
                     /locus_tag="VEV43_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYP22693.1"
                     /translation="MAYLQELDAALSFARAGGRIAMRHFRTQLRSQQKADGTWVTEAD
                     WAVEAQIRLRIARAFPEHNVLGEEEGLTAAGGGPAVDGAPTWIVDPVDGTNNYMAGIP
                     IWATLVALVVGGETVAGVAHAPALQETYDAATEEGARCNGEPIGVADTSDLGDATVLF
                     ASVGGWKEREGFDELTRRARRTRGFGDFWGHCLVARGAAHVMVEPELSVWDVAALRPI
                     VAEAGGRLTGLDGGPWESGACITTNGPLHDEVLALLQG"
     gene            complement(3775..3856)
                     /locus_tag="VEV43_03895"
     tRNA            complement(3775..3856)
                     /locus_tag="VEV43_03895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3820..3822),aa:Leu,seq:tag)
     gene            complement(3884..4354)
                     /locus_tag="VEV43_03900"
     CDS             complement(3884..4354)
                     /locus_tag="VEV43_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22694.1"
                     /translation="MGPDPAKRFMKKNGDRIPGGEQVQAVLIAEAKGGAWRRGMAAAG
                     PATATIGHDAKEKARAEQTAASAGGDAAQWPAASMFWLVLTDKQLHVFEGRTNSSDVA
                     GSQSYPRERIASIDLDKKLLISKLNVAFRDGSSVELDVAKQKTGPFVDAIRAGA"
     gene            complement(4405..5361)
                     /locus_tag="VEV43_03905"
     CDS             complement(4405..5361)
                     /locus_tag="VEV43_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYP22695.1"
                     /translation="MTPLRLLGLVLSFLLTASCSSGPATGAGAQGSPSVSPSGTPSPS
                     PSESPSPEPEPEPEPFVPAANARLPRRAPALAQRLTDVTLALRTSVAEWVESEETTKR
                     KPPRRVTLQALYQQRAYRLLSRRPRLAGRVIARLDPGLRREARANTTAGARLRSLVTP
                     LKPPVRLKTGPAEAAGVLLRYYRRAERRFGVEWEVLAAVNYVETKFNRVRSSSSAGAQ
                     GPMQFLPSTWEAYGLGGDVHDPRDAILGAANYLHASGAPDDYRGALYAYNHSDAYVDA
                     VLLYANRMKRRPLSYYGYYSWQVFVITTKGDVRLTGPGLDRG"
     gene            5559..>5932
                     /locus_tag="VEV43_03910"
     CDS             5559..>5932
                     /locus_tag="VEV43_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22696.1"
                     /translation="MRVAAPPAPPPPRSVDGPATTSTAGLLPVSLLATLALLVLPGAT
                     VAAAGVVLGEPLAGPAGWAGTLAVSLLYMLAGAKLWARHPRSKDLSWGELVLWSWARR
                     RRAEGTLERSAEMFEAEVAGFL"
BASE COUNT          848 a         2109 c         2103 g          872 t
ORIGIN      
        1 tctgaaggaa gaggggatcg acacgctgat cctcggctgc acgcactatc cgttgctctc
       61 ggggatgatc cgctacgtca tgggggagga ggtcgtcctg gtctcgagcg ccgacgcgac
      121 ggccgccgac gtctacgcga agctccgcga cgacgacctg ctgaaggaca ccgtcgacct
      181 cggcgagcac cgcttcatcg tgtcgagccc cgaaggcatc tcgagcgagc tcggccgccg
      241 cttcctcggc ccggagttcc gcaacgccga gcaccgtccc tggggagacc ggtgacgacc
      301 aggatcacga tcctcggctc gtcgggacgc tttgcgaccg cgcaccgcgc gagctccggc
      361 tacctgctcg agatcgacgg acacaggctg tggctcgacg cgggggccgg gacgtggcgg
      421 aacctgctcg gccagtgtgc gttcgacgag gtcgacggcg tgatcctcac ccaccgccac
      481 ccggaccacg tgaccgatgt ctaccagctg cagcacgcgc tcctctacgg gccgaagggg
      541 cggctcccgg agatcccgtt gtgggcgccc gctcagacga tcgagtcgat gtccgcttat
      601 gacgacctcc acgacgcgtt cgagccgcac gtcgtcgccg gaggcgactc gatcgaggtc
      661 gccggcgcga cgatccgctt cgtctcgatg gctcatccgc ccgagaccgt cggcgtgcgg
      721 gtggagaccg ccgacggcgt cctcgcgtac tctgccgact ccggtgagga ggccgacttc
      781 gaggcactcg ccggcggcgc cgggctgttc gtgtgcgagg cgtcgaacca ggattccgac
      841 gacatctggg agggtcacct gagggcatca caagcagcac ggatcgcgac cggcgcagga
      901 gtcgagaggc tggtgctcac gcacctcccg gtcgagaggg acctggggct gtcgctcgcg
      961 gaggcgcagg ccgcggtgga cgggccgtcg gtgcagctcg ccgccgacgg ggactcgcac
     1021 gaggtcgggc ggtgacgcgc gcggacgggc gcgcgcccgg cgagctccgg cccgtcgcta
     1081 tgacgctcgg cttcatgccg tacgccgaag gctcgtgcct cgtcgagatg ggcgagacga
     1141 aggtgatctg caccgcgtcc gtcgagggct cggtcccgcg ctggatgcag gggcagggca
     1201 agggctgggt gaccgccgag tacgggatgc tcccgcgcgc gacgacgacc cgccggcgcc
     1261 gtgacgtcga gaagggcagg cccgacggca ggacgcagga gatccagagg ctgatcggtc
     1321 gttcgctgcg cgcggtcgta gacatggacg cgctcggcga gcggacggtc tgggtggact
     1381 gcgacgtctt gagagccgac ggcggcacga ggacggcctc gatcacaggt gcgttcgtcg
     1441 cgctggcgga agcgttccgg gggttggagg agaaggagtt ggtctcgaag tcaccgctgc
     1501 tggactcggt ctcggccgtg agcgtcggga tcgtcggcgg gacgccgtgc ctcgacctga
     1561 actacgtcga ggactccacc gcgcaggtcg acatgaacgt cgtgatgacg ggggccggca
     1621 agctcgtcga ggttcagggc acggccgagc agggcgcgtt cgaacgttcc gagctagacc
     1681 gtctgctcga cctcgcgacc gacggcatcg cgcagctgac cgcgcaccag cagcgcgcgc
     1741 tgggagcccc ggccgccgag tgaaggccgt gctcgcctcg cgcaaccgcc acaaggcgga
     1801 acaggtcgcg ctgctgctgc cggacctcga gctcgtgccc ctcgacgacg tcgcgccgga
     1861 cctcgagctg gcggagccgt acgacacgtt cgaggacaac gcgctcgcga aggcgcgggc
     1921 ggtcgtggcg gccaccggtc tcccggccgt cgcggacgac tcgggcctgg aggtcgacgc
     1981 gctcgggggc gcgcccggtg tcttctcggc tcgctacgcg ggagacgacg cgaccgacga
     2041 ggcgaacaac gagaagctcg tcgacgccct ggccggcacg cccgaagacg acaggtcctg
     2101 ccgctaccgc tgcgtcgcgg tcttcgtcgc gcccgacggc cgcgagctcg tggcgtcggg
     2161 ctcgtgcgag gggacggtgg tgctggaggg acgagggacg ctcggcttcg gctacgaccc
     2221 gcacgtgatc cccgagtgcg cgacgaggac gatgggcgag atcccgctcg acgagaagct
     2281 gaggttcagc caccggggcc gcgccttccg cgcgctcgcg gccatgctcg gccgatgagc
     2341 gacctcgagc gcgtcagcga gtccatcgcg cacctgcgac gcgaccacgt cgtgcaaccg
     2401 ctcgagatcg aggacctcga ccccgacccg atcgtgcagt tcggcaagtg gctggaggag
     2461 gctctcgccg aaggcttgat gctcccgaac acgatgacgc tcgcgacggc gacgcccgag
     2521 gggcgcccgt cggcccgcat ggtcttgctc aagggcttcg accacgacgg cttcgtcttc
     2581 tacacgaact acgagagccg caaggcccgg gagctgtcgg cgaacccgca tgcggcattg
     2641 gtcttctact gggcgcgcct cgagagacag gtgcgcgtcg agggccgcgt cgagcgcgtg
     2701 cccgccgagg agtccgacga gtacttcgcg tcgcggcctc tcgagagccg tctcggggca
     2761 tgggcgtcgc ggcagagcgc gccgctcgcg tcgcgcgccg agctggagga gcgcgtcgcg
     2821 cgactcgaac agcagtacac gtcgggcgag gtgccgcggc ccccgcactg gggcggctgg
     2881 ctcgtgcggc ccgagtcgat cgagctctgg cagggccggc cgaaccgctt gcacgaccgc
     2941 ttcctgtact cgtgcagcga cgacgggtgg gagcgcgtcc gcctctatcc ctgaagcagc
     3001 gcgaggacct cgtcgtgcag ggggccgttc gtggttatgc aggcgccgga ctcccacggc
     3061 cctccgtcga gcccggtcag ccgcccgccc gcctcggcga cgatcggccg gagcgcggcg
     3121 acgtcccaca ctgagagctc cggctcgacc atgacgtggg ccgcgccccg agcgacgagg
     3181 cagtgccccc agaagtcccc gaaccctcgt gtccgccgcg ctctccgcgt gagctcgtcg
     3241 aacccttctc gctccttcca tcccccgacg cttgcgaaca gcacagtcgc gtcgcccagg
     3301 tcggaggtgt ccgcgacgcc gatcggctcg ccgttgcagc gcgcgccttc ttccgtcgcc
     3361 gcgtcgtacg tctcctgcag agcgggcgca tgcgcgacac cggcgaccgt ctcgccgccg
     3421 accacgagcg cgaccagcgt cgcccagatc gggatgccgg ccatgtagtt gttcgtgccg
     3481 tcgacggggt cgacgatcca cgtcggcgcg ccgtctacgg cagggccccc gcccgcagcc
     3541 gtgaggccct cctcttcgcc gaggacgttg tgctcgggga acgcccgtgc gatgcggagt
     3601 cggatctgcg cctcgaccgc ccagtccgct tcggtgaccc aggtgccgtc ggccttctgt
     3661 tgcgaccgga gctgcgtacg gaagtgccgc atggcgatcc gcccacccgc ccgagcgaaa
     3721 gacagcgccg cgtcgagctc ctgtaggtag gccacggcga caccttatgg ggagtgcgag
     3781 aggggggact cgaaccccca cgtccggaga caccggcacc taaagccggc gcgtctgcca
     3841 attccgccac tctcgcagcg gctccgatgt tagagccgcg ccgtcaggcc cccgcgcgga
     3901 tcgcgtcgac gaacggtccc gtcttctgct tggccacgtc cagctcgacc gacgacccgt
     3961 cacggaaggc cacgttcagc ttcgagatca agagcttctt gtcgaggtcg atcgacgcga
     4021 tccgctcgcg cgggtagctc tgcgaaccag cgacgtcgga gctgttcgtg cgcccctcga
     4081 acacgtggag ctgcttgtcg gtgaggacga gccagaacat cgacgccgcc ggccactgag
     4141 ccgcatcgcc tccggcggac gcggcggtct gctcggccct cgccttctcc ttcgcgtcgt
     4201 ggccgatggt cgccgtcgcg ggccctgccg cggccatgcc ccgacgccaa gcaccgccct
     4261 tggcctcggc gatgagcacg gcctgcacct gctcgccgcc ggggatgcga tcgccgttct
     4321 tcttcatgaa ccgcttcgcc ggatccggtc ccaccgcagc ctcctccgtc tcgagatgcc
     4381 ctgccccaag agtaggtcgg tttgctaccc gcggtccaga cccggccccg tcaggcggac
     4441 gtcacccttc gtcgtgatga cgaagacctg ccacgagtag tagccgtagt agctcagcgg
     4501 ccgtcgcttc atccggttcg cgtagaggag gactgcgtcc acgtacgcgt cggagtggtt
     4561 gtaggcgtag agcgcccccc ggtagtcgtc cggcgccccc gatgcgtgca ggtagttcgc
     4621 ggcccccagg atcgcgtcgc gagggtcgtg gacgtccccg ccgagcccgt acgcctccca
     4681 cgtcgagggc aggaattgca tcgggccctg agccccggcc gagctgctcg accggacccg
     4741 gttgaacttc gtctcgacgt agttcacggc ggccagcacc tcccactcca cgccgaagcg
     4801 ccgctcggcc ctccggtagt agcgcaggag caccccggcg gcctcggcgg gcccggtctt
     4861 cagccgcacc ggcggcttca gcggcgtcac cagcgagcgc agcctcgccc cggcggtcgt
     4921 gttcgcgcgg gcctcgcggc ggagacccgg atccagccgt gcgatcaccc ggccggcgag
     4981 gcgcggccgc cgcgagagca gccggtacgc ccgctgctgg tacagcgcct gcaaggtcac
     5041 ccgccgcggg ggcttccgct tcgtcgtctc ctcggactcg acccattcgg cgaccgaggt
     5101 cctcaacgcg agcgtgacgt ccgtcagcct ctgcgcaaga gcgggagccc ggcgggggag
     5161 ccgcgcgttg gccgcgggca cgaacggctc cggctccggt tcgggctccg gcgagggcga
     5221 ctccgacggc gacggcgacg gagtccccga cggcgagacc gagggcgagc cctgggcccc
     5281 cgcccccgtc gccggtccgc tgctgcacga agcggtcagc aagaacgaca agacgagacc
     5341 gagaagccgg aggggagtca ccgggcgatc gtatgtccgt ccggaggctc tggtgcgtgt
     5401 ggttcgtctg gtacgtgtgg ttggaggtcc aaccaccgcg accaccgcga ccacgttgac
     5461 cacgagcctc gggcacgaac gatgatggtt tgacttccag tgatttccaa gaagcttcta
     5521 taggttccgg ccgctgcggc cgaaaccaaa gcatggccat gcgcgtcgcg gctccgcccg
     5581 cgcctcctcc gccccgctcg gtcgacggcc cggctacgac ctctacggcc gggctgctgc
     5641 cggtctcgct gctcgccacg ctcgcgctcc tggtcctgcc gggggcgacg gtcgcggcgg
     5701 ccggcgtcgt gctgggcgag cccctggccg ggcctgcggg gtgggccgga acgctcgccg
     5761 tgtcgctcct ctacatgctc gcgggggcca agctgtgggc ccggcatccg cgttcgaagg
     5821 acctctcgtg gggcgagctc gtgctttgga gctgggcccg ccgccgtcgc gccgagggga
     5881 cgctcgagcg gagcgccgag atgttcgagg ccgaggtcgc cgggttcctc aa
//
LOCUS       DASQDO010000129         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_196862, whole genome shotgun sequence.
ACCESSION   DASQDO010000129 DASQDO010000000
VERSION     DASQDO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196862"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VEV43_03915"
     CDS             complement(<1..699)
                     /locus_tag="VEV43_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYP22697.1"
                     /translation="MSIDPRNLPEMPSLMIAGPGELHEEELKVLGSQVIAHYGDVWTG
                     LHNDAVAAVGELVGAADAPYVVPGTGTTCRDAAVFNVFEPGQRVVVAHTGFFGNRLLE
                     IARQHRLEVTEVPVDVGAPVNPGRVADVVDGADGVLLCHVDTATGVRHPIAEVAAVAN
                     GAGAASVVDGIASVGGDLVDVDGMGLAALVTGSQKGLEAPPGLGILALGSRGRERVEA
                     RSSRPESWYLDLKVW"
     gene            822..2201
                     /locus_tag="VEV43_03920"
     CDS             822..2201
                     /locus_tag="VEV43_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22698.1"
                     /translation="MRRPFALLMSLLTGIVLGAVPAASAAPDRAQAPEAALMSDNVEL
                     LTALPNPGIIGARFRDGLMYATSTTGLTIYDVSDARAPKEVGRLPLPHFENEDVDLGG
                     DILLISNDAAESTGILYVIDVSDPAAPAILTPFPMGGNPVEGGPGHTASCILECKFAW
                     VTDGAAIKVIDLRDPAAPVDMGTFETPAGGEIATHDVQVDKQGLAWVVGFGGAVAYRL
                     PANYDGNGLGQVAVKTDPTGNSTYLEELGLGDGSKPNDYVLHNSMRVKKGVVYVTEED
                     YTRPGCRGAGSFETWKLPLRANGTPSGEDMTLMDSWETELLADTANAAAMCSAHYFDV
                     RKNVVAQGWYEQGLRLLDVSKAGAVRQVGYFVPPSAMTWAAYFPPTDKSGRVLYVLDA
                     THGIDVLEFDRPSTGPLSASGKACTGRRCAFRVAPTVRAPIRADWRAVAPATGTPLAD
                     FGYACRIRL"
     gene            2323..>3355
                     /locus_tag="VEV43_03925"
     CDS             2323..>3355
                     /locus_tag="VEV43_03925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYP22699.1"
                     /translation="MKAGERVQGRYELIEPLGRGGMAEVWLARDERLERKVAVKFMAA
                     NLCHDPEFLVRFFSEAQAVARISHPNVVSVLDFGDFEDRPYLVMECAPGGSLADLTGE
                     PMLPGRAAEIVAAAARGAGAAHRAGLVHRDVKPANILLDETGEPKLADFGIASSAPSE
                     NLTATGTAIGSPHYVSPEQASGRAVTPASDVYSLGAVLYELLTGAPPFDADNVTAIAI
                     AHVEQPPVPPSARVEGLPAHLDDLVLSCLAKDPAARPPDGDALAATLENANATSTAWI
                     PAVAPAALEATAYEDDYEYETPVKKRWLVPALLIASLGLALAGTMYVSGRPDDVAEAG
                     TRETPATAEP"
BASE COUNT          531 a         1231 c         1129 g          464 t
ORIGIN      
        1 ccagaccttg aggtcgaggt accaggactc cggacgggag ctccgcgcct cgacgcgttc
       61 tcgcccccga gaacccaacg ccaggatccc gagccccggc ggggcctcga gccccttctg
      121 cgaacccgtc acgagcgcgg ccagtcccat cccgtcgacg tcaaccaggt cgccgccgac
      181 cgaggcgatc ccgtcgacca cgctcgcggc ccccgcaccg ttcgcgaccg cagcgacctc
      241 ggcgatcgga tgacggacgc cggtcgccgt gtcgacgtga cacaaaagga cgccgtcagc
      301 gccgtcgacc acgtctgcga cacgcccggg gttgaccggc gcaccgacgt cgaccgggac
      361 ctcggtcacc tcgagacgat gctggcgcgc gatctccaac agacggttcc cgaagaaccc
      421 ggtgtgcgcg acgacgacgc gctgtccggg ctcgaagacg ttgaacaccg ccgcgtcgcg
      481 gcacgtcgtc cccgtgccgg ggacgacgta gggcgcgtcg gccgcgccga cgagctcgcc
      541 gaccgcggcg accgcgtcgt tgtgcaggcc cgtccagacg tcgccgtagt gcgcgatcac
      601 ctgcgagccg agaaccttca gctcttcttc gtggagctca cccgggcccg cgatcatcag
      661 cgagggcatc tcggggaggt tcctggggtc tatcgacatt gacaaacctc cgggggggtc
      721 ggttggcagg ttccagccta ttccgtggaa ccccgtagac gcgatgaagg cgccccctta
      781 gcgtggaaag ccccacctac ggacgacttc ggaggactgc tatgcggcga cccttcgcgc
      841 tacttatgtc ccttctgacc gggatcgtcc tcggcgcggt accggctgca tcggcggcgc
      901 ccgaccgcgc gcaggcaccg gaggccgcgc tcatgtcgga caacgtcgag cttctgaccg
      961 cattgccgaa cccggggatc atcggcgcgc gcttccgcga cggcctcatg tacgcgacgt
     1021 cgacgaccgg gctgacgatc tacgacgtct cggacgcccg tgccccgaaa gaggtcgggc
     1081 gcctcccgct cccccacttc gagaacgaag acgtagacct cggcggcgac atcctgctga
     1141 tcagcaacga cgccgcggag agcaccggca tcctgtacgt gatcgacgtc tccgacccgg
     1201 cggcccccgc gatcctcacg ccgttcccga tgggcggcaa cccggtcgaa ggggggccgg
     1261 gccacaccgc gagctgcatc ctcgagtgca agttcgcgtg ggtcaccgac ggagcggcga
     1321 tcaaggtcat cgacttgcgc gaccccgccg ctccggtcga catgggcacg ttcgagacgc
     1381 cggccggcgg agagatcgcg acgcacgacg tccaggtcga caagcagggc ctcgcgtggg
     1441 tcgtcggctt cggcggcgcg gtcgcgtacc ggctcccggc taactacgac ggcaacggtc
     1501 tcggtcaggt cgccgtcaag accgacccga ccggcaacag cacgtacctc gaagagctcg
     1561 ggctcggcga cggctccaag cccaacgact acgtcctcca caactcgatg cgcgtaaaga
     1621 agggcgtcgt gtacgtgacc gaggaggact acacgcggcc cggttgccgc ggcgccggtt
     1681 cgttcgagac gtggaagctg ccgctgcgcg cgaacggcac gccgagcggc gaggacatga
     1741 cgctgatgga ctcctgggag accgagctgc tcgcagacac cgcgaacgcc gcagcgatgt
     1801 gctcggccca ctacttcgac gtccggaaaa acgtcgtcgc gcagggctgg tacgagcagg
     1861 ggctccggct cctcgacgtg agcaaggccg gcgcggtcag gcaggtcggc tacttcgtcc
     1921 cgccgagcgc gatgacgtgg gccgcgtact tcccgccgac cgacaagagc ggccgcgtcc
     1981 tctacgtcct cgacgcgacg cacggcatcg acgtcctcga gttcgaccgg ccgtcgaccg
     2041 gccccctgtc ggcctcgggg aaggcctgca ccggacgccg ctgtgcgttc cgggtagcgc
     2101 cgacggtgcg ggcgccgatc cgggccgatt ggcgcgcggt agctccggca acggggactc
     2161 cgctggccga cttcggctac gcctgccgca tcaggctcta gcgggatcgt ggcccgtgag
     2221 gttgcctccc aacctcacgg gccacctctt gcccactaca tatagggacg cttcggcgac
     2281 gtagcgtttc cagagcaggc ggcctgggaa agatcacctt ccgtgaaggc gggagaacgc
     2341 gtccaggggc gctacgagct gatcgagcca ctcgggcgcg gcggcatggc cgaggtgtgg
     2401 ctcgcccgcg acgagcggct cgagcgcaag gtcgccgtga agttcatggc ggcgaacctc
     2461 tgccacgacc ccgagttcct cgtccggttc ttctccgagg cgcaggccgt cgcccgcatc
     2521 tcgcacccga acgtcgtctc ggtcctcgac ttcggcgact tcgaggaccg cccctacctc
     2581 gtgatggagt gcgcccccgg gggctcactg gcggacctga cgggcgagcc gatgctgccg
     2641 ggaagggccg ccgagatcgt cgcagcggcc gcccgcggag cgggggccgc gcaccgggcg
     2701 gggctcgtcc accgagacgt caagccggcg aacatcctgc tcgacgagac cggcgagccg
     2761 aagctcgccg acttcggcat cgcgtcgtcg gcgccgtccg agaacctgac cgcgaccggt
     2821 accgcgatcg ggtcaccgca ctacgtctcg cccgagcagg cctcgggccg cgccgtcaca
     2881 ccggcgtccg acgtgtactc gctgggcgcg gtgctctacg agctgctgac gggagcgccg
     2941 ccgttcgacg ccgacaacgt caccgcgatc gcgatcgccc acgtcgagca gcctcccgtc
     3001 ccgccgagcg cacgcgtcga gggcctgccc gcgcacctcg acgacctcgt cctgtcgtgt
     3061 ctcgccaagg acccggcggc ccggcccccc gacggcgacg cgctggcggc cactctggag
     3121 aacgccaacg ccacgtccac tgcgtggatt cccgcagtcg cgccggccgc gctcgaagcc
     3181 actgcctacg aggacgacta cgaatacgag acgccggtga agaagcggtg gctcgtcccg
     3241 gcgctcctga tcgcgtcgct gggactcgct ctcgccggga cgatgtacgt gtcgggccgc
     3301 cccgacgacg tcgccgaagc cgggacgcgg gaaacccctg ccacggcgga gccgg
//
LOCUS       DASQDO010000130         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_441531, whole genome shotgun sequence.
ACCESSION   DASQDO010000130 DASQDO010000000
VERSION     DASQDO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441531"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VEV43_03930"
     CDS             complement(<1..180)
                     /locus_tag="VEV43_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22700.1"
                     /translation="MHTRPGRRERALVALVVALACAGAGAHAAAAPPPRLLPDLVPQS
                     PYEVVGPTTGFSLGLG"
     gene            complement(238..1281)
                     /locus_tag="VEV43_03935"
     CDS             complement(238..1281)
                     /locus_tag="VEV43_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22701.1"
                     /translation="MTRAVGAALVLALLSSSAVAAGDPVIGYWTDTNVIIADAEGEQV
                     RELPDFRRFSFSGSLLAGDVQGKRAVGYDLGSGDRTFTIAHASNPVVAAARDVVAFLP
                     TFRRDEYARSVWMRTARGQVRKVAQFRAPGVDGIRHGMGGDGAPLEVAFDDRGRYLAI
                     VFGLEPLRSFDVWVVDTKTRAAARMTRGENSHNPSLSPGGEQLAVRVERPESCPDPVY
                     GEILIGKIRVIERTTRTARDLTAFDCDLFYDTPRWIDDETLVAVRVTKDPEETYGYDL
                     DLVRIDASSGEVTELLTAGNPCCITASPSLGKVAYGFSDRAGFAVLEVATGEVVEFDA
                     GAYVPHLAGEGRS"
     gene            1354..2139
                     /locus_tag="VEV43_03940"
     CDS             1354..2139
                     /locus_tag="VEV43_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HYP22702.1"
                     /translation="MRSALACLLVALGTVACDSSPESEAGRSAPPSASESPAPTRRPV
                     HSPEPGMPYEGDWTLVSGTSPVGPIPIDPDYAITLVLTERQAGGRATCNGYGGGFKID
                     GTAFHAEGFGADQAGCATEELDEAETRYFAALVEIDEIHADETTLHLTGPGSELVFER
                     LPEPDLDAVVDRTWVSGDHTLLLSSDRTFRISYGCDVLKGTWDVMAGRIYMPEARRVA
                     DCPREPRTQVDVDVSSGDFSVSVEGDRLTISHGGKEVVYRAER"
     gene            2285..2698
                     /locus_tag="VEV43_03945"
     CDS             2285..2698
                     /locus_tag="VEV43_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22703.1"
                     /translation="MKRTNLGLALAAVVTVVAVAPAAGAPRTRTETVAYERASGAHLS
                     EDVSVDVAAGEMPEAAPMAREKTVSITLEDDSGRPIAGVAHQGDDELGAFCGQTEAPL
                     TLVSRKPVHVHVYSGEGCSDVSVPTAGTVAFTFAR"
     gene            2805..>3325
                     /locus_tag="VEV43_03950"
     CDS             2805..>3325
                     /locus_tag="VEV43_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22704.1"
                     /translation="MRKRASLFLAAGLLAATAVPGGNAFANHGGNLTIQVAAPLDDVP
                     GESLRFLAPYTIRVHRGDTVTFDLHNGHSAGLLPEDESAQDWLDENWYSPTGQYTPVA
                     QDDEAGELIDNFHNIDTPSDATCGGSGETACEYDGNELVYSGTVFGVDVPPGGQFPET
                     MTFATTIQAQPGTR"
BASE COUNT          521 a         1160 c         1177 g          467 t
ORIGIN      
        1 gcccagcccg agcgagaacc cggtcgtcgg gcctaccacc tcgtacggcg actgcgggac
       61 gaggtccggg agcagccggg gagggggtgc cgccgcggca tgcgcgcccg cgccggcgca
      121 ggcgagcgcg acgacgagcg ccacgagggc gcgctctctc ctgccgggtc gtgtgtgcat
      181 gcgtcggaag tcttcgccgg ggcgcggcga cgcccctgcg gcaggactgt gacgtagtta
      241 ggagcggccc tctccggcca ggtgcggcac atacgccccc gcgtcgaact cgacgacctc
      301 gcccgtcgcg acctcgagga cggcgaaccc tgcccggtcg ctgaagccgt acgcgacctt
      361 cccgaggcta ggcgaggccg tgatgcaaca cgggttgccc gccgtgagga gctcggtgac
      421 ctcccccgag gacgcgtcga tccggaccag gtcgagatcg tagccgtacg tctcttcggg
      481 atccttcgtg acgcgcacag cgacgagcgt ctcgtcgtcg atccagcgag gcgtgtcgta
      541 gaagaggtcg cagtcgaatg ccgtcaggtc gcgtgccgtg cgggtcgtgc gctcgatcac
      601 cctgatcttg ccgatgagga tctcgccgta caccgggtcg gggcagctct cggggcgctc
      661 gacgcggacg gccagctgct cgccgccggg cgacagcgac gggttgtgcg agttctcccc
      721 tcgggtcatc cgcgccgccg cccgtgtctt cgtgtcgacg acccagacgt cgaacgagcg
      781 caacggctcg aggccgaaca cgatcgcgag gtagcggccc cgatcgtcga acgcgacctc
      841 gagtggcgcg ccgtctccac ccatcccgtg ccggatcccg tcgacgccgg gcgctcggaa
      901 ctgggcgacc ttccggacct gcccgcgggc cgttcgcatc cagaccgacc gcgcgtactc
      961 gtcccgccgg aacgtcggga ggaacgcgac gacgtcgcgc gcagcggcga cgacgggatt
     1021 gctcgcgtgg gcgatcgtga aggtgcggtc gccggacccg aggtcgtaac cgaccgcgcg
     1081 cttgccctgg acatccccgg cgagcaacga ccccgagaac gagaagcgcc ggaagtccgg
     1141 gagctcacgg acctgttcgc cctccgcatc cgcgatgatg acgttggtgt cggtccagta
     1201 cccgatcacg ggatcgccgg cggcgaccgc ggacgaagac agcagcgcca gcacgagcgc
     1261 cgcgccgact gctcgcgtca tcggacctcc ctccgtgatg ccgccccggg gattgtccgg
     1321 caggatcgca gcgtcgccaa gggggaaacg cccatgagat ccgccctcgc ctgtctgctc
     1381 gtcgcgctgg ggacggtcgc gtgcgactcg tcgcccgaga gcgaagcggg tcgctccgcg
     1441 ccgccctctg cgagtgaatc gcccgccccg acgcgccgtc cggtgcattc gcccgagccc
     1501 gggatgccgt acgaaggcga ctggacgttg gtctccggca cgagcccggt agggcccatc
     1561 cccatcgacc cggactacgc catcacgctc gtcctcacgg agaggcaggc cggcgggcgc
     1621 gctacctgca acggctacgg cgggggcttc aagatcgacg gcaccgcgtt ccacgccgag
     1681 gggttcggcg ccgaccaagc cgggtgcgcc acggaggagc tcgacgaggc agagacccga
     1741 tacttcgcgg cgctggtcga gatcgacgag atacatgcgg acgagacgac gctccatctg
     1801 acgggccccg gctcggagct cgtgttcgaa cggcttcccg aacccgacct cgacgccgtc
     1861 gtcgaccgca cctgggtgtc gggtgaccac acgcttctcc tgtcgagcga ccgcacgttc
     1921 cgcatctcgt acgggtgcga cgtgctgaag gggacgtggg acgtgatggc gggccggatc
     1981 tacatgcccg aggcgcggcg cgtagcggac tgtccgcgcg aaccccgcac ccaagtcgac
     2041 gtcgacgtct cgtcggggga cttcagcgtg tccgtcgagg gcgaccgcct gacgatctcg
     2101 cacggcggca aagaggtcgt gtaccgggcc gagcgttaga tctcgcgcgc cgagaggagc
     2161 gcgcgtccgc catcacccgc gtggcggtga gacgacgcga ccgcgtcacc ggcggcggga
     2221 cctcgttacg gcggtgcgga cccggcgtag acgaggtaca gacattcgac gcgaggaggt
     2281 tgtcgtgaag cgaacgaacc tgggcctggc tctcgcagcg gtcgtgacgg tcgtcgcggt
     2341 cgcgcccgcg gccggcgctc cccggactcg taccgagacc gtcgcttacg agcgagcgtc
     2401 cggagcgcat ctgtcggagg acgtgtcggt ggacgtcgcc gccggcgaga tgcccgaggc
     2461 ggcgcccatg gcgcgcgaga agacggtgtc gataacgctg gaggacgact ccggccggcc
     2521 gatcgcaggt gtggcgcacc aaggcgacga tgagctcggg gccttctgcg ggcagaccga
     2581 ggcgccgttg acgctcgtca gccggaagcc ggtccacgtg cacgtctact cgggcgaagg
     2641 atgctctgac gtcagcgtgc cgaccgccgg aaccgtcgcc ttcaccttcg cgaggtagcg
     2701 ccaccgcccg acttgcgcgt tccggacaaa tcggaaatgg cccctatcgg cggtcacgga
     2761 tgggacaatc gcccggtcca gttgaccttc gaagggagtc ccccttgcgc aagcgcgcat
     2821 ctttgttcct cgcagccggg ctgctcgcgg cgacggccgt gcccggcggc aacgccttcg
     2881 ccaaccacgg cgggaacctc acgatccagg tggccgcgcc gttggacgac gtcccgggcg
     2941 agtcgctgag gttcctcgcg ccgtacacga tcagggtcca ccgcggggac accgtgacgt
     3001 tcgacctcca caacgggcac tcggcggggc tgctgccgga ggacgagagc gcgcaggact
     3061 ggctcgacga gaactggtac tcacccaccg gtcagtacac gccggtcgcg caggacgacg
     3121 aggccggcga gctgatcgac aacttccaca acatcgacac gccttcggat gcgacgtgcg
     3181 gcggctcggg cgagaccgcg tgcgagtacg acggcaacga gctcgtctat tcggggacgg
     3241 tgttcggcgt ggacgtgcct ccgggcggtc agttccccga gaccatgacg ttcgcgacga
     3301 cgatccaggc ccagcccgga acgcg
//
LOCUS       DASQDO010000131         7633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_258170, whole genome shotgun sequence.
ACCESSION   DASQDO010000131 DASQDO010000000
VERSION     DASQDO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258170"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..264)
                     /locus_tag="VEV43_03955"
     CDS             complement(112..264)
                     /locus_tag="VEV43_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22705.1"
                     /translation="MPSETRDRAMQFVDLMLERVEITKYPSKDLMDRIERTLVLFWEE
                     EQAEGR"
     gene            440..919
                     /gene="gvpJ"
                     /locus_tag="VEV43_03960"
     CDS             440..919
                     /gene="gvpJ"
                     /locus_tag="VEV43_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HYP22706.1"
                     /translation="MAATAATRLQQTHLAQQSSGRGGPSGLADVLEVLLDKGLVIDAF
                     VRVSVVGIELLTIDARIVVASVDTYLRFAEAVNRLDLLQAGNSQSLGDLTSGGGGSRA
                     LKSRPSRGEPEAEREDEPEPEHEDEPEKDDERRAEGDDDADDDKGILTRIKERGQVF"
     gene            933..1217
                     /locus_tag="VEV43_03965"
     CDS             933..1217
                     /locus_tag="VEV43_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HYP22707.1"
                     /translation="MFGRKKGLDRARGVLDDAGSGARKALSSKKPNKVDSVTAAALLL
                     GFANWVSVSLFNFDLVQAVAKKKSVPGRAAYGFLGLSALYASIRGTRGVK"
     gene            1254..1598
                     /gene="gvpJ"
                     /locus_tag="VEV43_03970"
     CDS             1254..1598
                     /gene="gvpJ"
                     /locus_tag="VEV43_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HYP22708.1"
                     /translation="MSRGGPSGLADVLEVLLDKGLVIDAFVRVSLVGIELLTIDARIV
                     VASVDTYLRFAEAVNRLDLVQANNSQTLGDLVSGSKEKGAKHVTKGALEGIREKLPGG
                     GKEEREHEEARS"
     gene            1602..2348
                     /locus_tag="VEV43_03975"
     CDS             1602..2348
                     /locus_tag="VEV43_03975"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HYP22709.1"
                     /translation="MPETPLYVYGIVRSDATLPGGAPRGVAGGSVRLLEDGSLAAVVT
                     DLPSSSYEAGRGDLLAHSEVLQHAFASADVLPMAFGTAFVSQGELAETFLRPNHDALV
                     AMLDSVGGMAEVQVRADYEQDAIARDIAESDRSVRKLQARARERGDVETKIELGRRFA
                     ATLENKRYADARALVDALSKAARDVSLGEAPGEFGLLRASFLVPRDGLDRFDSAADTA
                     LASLGGRATLRCVGPLPPYSFVDASALVVA"
     gene            2348..2593
                     /locus_tag="VEV43_03980"
     CDS             2348..2593
                     /locus_tag="VEV43_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HYP22710.1"
                     /translation="MGLFSNVLLLPLAPVRGVLWVAQVLADVASKELNDPQTLRARLR
                     EAEDAHRRGEISAEDLDRIEDAVFSQLVALQQRGGVS"
     gene            2590..2856
                     /locus_tag="VEV43_03985"
     CDS             2590..2856
                     /locus_tag="VEV43_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HYP22711.1"
                     /translation="MSERLSAGEAIRAARDVVVELTGKQVEGISGINRSDGGWAVSLD
                     VVELARIPSSTDLIATYEVCLDDDGGLVDMERRRRYTRNQTDEE"
     gene            2860..3213
                     /locus_tag="VEV43_03990"
     CDS             2860..3213
                     /locus_tag="VEV43_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HYP22712.1"
                     /translation="MQLQNQPGARPPASAGGGDLADILERVLDKGIVVAGDVAINLLD
                     IELLTIKLRLLIASADTAKKMGIDWWESDPFLSGKAGSELPEGNGNGKGGALEERLDR
                     IENALAQLAKPPEER"
     gene            3210..3965
                     /locus_tag="VEV43_03995"
     CDS             3210..3965
                     /locus_tag="VEV43_03995"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HYP22713.1"
                     /translation="MTPGLYVYGIVATGSVPGPLREHGVGDGPPVLFDVDGLSAIVSG
                     IDVSEYEGAALERNAATPEWLELKVRGHEAVVEGVLAHATVVPMRFGSIFSSTESLQK
                     MLTEHADALREALTRVEARTEWGVKVHCDRSRLVSALVGSSPEPDSGRGYLMQKKARL
                     EADARAAEAAAEIAAEAHAALAAVASESVAAPPRGEEILNGAYLVDEAGRDEFMRKVE
                     ELQRRHEGAFAFEVTGPWPPYSFTSADVGGPRP"
     gene            3962..4132
                     /gene="gvpJ"
                     /locus_tag="VEV43_04000"
     CDS             3962..4132
                     /gene="gvpJ"
                     /locus_tag="VEV43_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HYP22714.1"
                     /translation="MSADLQRQVTLLDLVDRIVDKGAVVRGQVVLAVADVELVKLDLS
                     LLLASVETLEGR"
     gene            4129..4440
                     /locus_tag="VEV43_04005"
     CDS             4129..4440
                     /locus_tag="VEV43_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006867694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HYP22715.1"
                     /translation="MSSPVPALAEVVGDLERVRIHADPSNAERGLAQLVLTIVELLRQ
                     LMERQAIRRMESGSLTDDEIERMGETFMQLERRMTELREHFGLAPEDLNLDLGPLGKL
                     L"
     gene            4457..4864
                     /locus_tag="VEV43_04010"
     CDS             4457..4864
                     /locus_tag="VEV43_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HYP22716.1"
                     /translation="MIEKIARRAGGFASSTALRWIRTGADEVAEIAPTRNGSHKGGGR
                     AVAGAAAGAVALITSSKVARSGIERGLRALADAVRTSSSTSHGNGNGNGNGNGSKDLT
                     AKTRAELYELAKKADLPGRSSMSKDDLAKALKG"
     gene            complement(4925..5347)
                     /locus_tag="VEV43_04015"
     CDS             complement(4925..5347)
                     /locus_tag="VEV43_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HYP22717.1"
                     /translation="MSTTVGDKTPAQLFALVFGVIYLVVGLVGFAVTGFDNFASEVPG
                     EKLIVFAINPLHNIAHLGVGVLLLIGSSRHETAKSVNLMVGIVYLLLAILGFAGILIE
                     DLLNINSADNFLHIATAALAIFFGTAGATARTGAARTA"
     gene            complement(5668..7272)
                     /locus_tag="VEV43_04020"
     CDS             complement(5668..7272)
                     /locus_tag="VEV43_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP22718.1"
                     /translation="MKRVLLLGLDGMTYSVLEPAFEAGHMPVFKRLLDRGASGVLTST
                     VPPYTPPGWTSIFTGVNPGKHGIFGFTLGNVQSSKGLVSLDRVTAPAIWNAANAQGVR
                     MGLFNIPMTYPAPAVDGFAVSGMLTPEGGGKTPESFTHPESLSAEIAGVVPGYEIDIE
                     VDYDQDWKSTAIIERLSANLDKKRLALAHLLEQHGDLGLLFGVLEAPDRLMHVHYKYI
                     DPRQEHFHRPEAAPIRERAWEFFDEMDGMIGDMLAWAGNDGFVVTMSDHGFGPKDKSV
                     NVNLALREWGLLSIGGAGAVTKSAGVRTLARKAKKVLPKSVWQKAKGAAQSSIDWSRT
                     KAFSAPIPQQGIYVNLQGREPHGIVPESEYEAGRDEIVERFSALVDPDDGKPVLDRIY
                     RREEVVSGPQAVDAPDLFPVCREYSYELSDGLYSPRVLDDYRGLPRGFHHMDGIFGIA
                     GPGIEPASGLKASLYDIMPTALHLAGLKVPEVDGRVLTEWLPDGPAPVVEAMDLPLAG
                     SGAEASPYSAEDEAAIEESLRNLGYL"
     gene            complement(7304..>7633)
                     /locus_tag="VEV43_04025"
     CDS             complement(7304..>7633)
                     /locus_tag="VEV43_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22719.1"
                     /translation="SASLGLALWAWRSDAAEPSDVLWVVVYAVLAYYFIEISGAEKAD
                     SPGGVAGRLSGALARRRLLPNPGMPDVQAVVFILGPITGLVVPALGLGIAMVTAAILL
                     TAWRRLR"
BASE COUNT         1247 a         2621 c         2672 g         1093 t
ORIGIN      
        1 ggcgtcgcga tctcgtcggc ctacgtcgcg aagcgcgcag gcggcgagag cgagctgatc
       61 ctggcggtcg acgacaaaga ggccgcgtcc ggcgcctgac ccgaagggct gttaacggcc
      121 ttcggcctgc tcctcttccc agaacagcac gagcgtccgc tcgatccggt ccatgaggtc
      181 cttgctcggg tacttcgtga tctcgacgcg ctcgagcatg aggtcgacga actgcatcgc
      241 tcggtctcgg gtctcggacg gcattcggtc ccccttcctg tagatgcgtc gcgcatccaa
      301 tctgtgacgc tgcgtacccg ccggtgacgc tccgaaacca cccaaacaca tggcgtgatt
      361 ttccagctac gtaacgtgtt tgcacggcta cggagcgggt atccagggtg aagccgccgg
      421 acctaagggg gactcacgga tggccgcaac cgcagcgaca cggctccaac agacccacct
      481 ggcccagcag agctcgggcc gcggggggcc gagcgggctc gccgacgtcc tcgaggtgct
      541 cctcgacaag ggcctcgtca tcgacgcctt cgtgcgcgtc tcggtggtcg ggatcgagct
      601 gctgacgatc gacgcccgca tcgtcgtcgc gagcgtcgac acctacctgc gcttcgcaga
      661 ggcagtcaac cgtctcgacc ttctgcaggc cggcaactcg caatcgctcg gcgacctgac
      721 ctccggcggc ggcggctccc gcgccctgaa gtcgcggccg tcgcgcggcg agcccgaagc
      781 cgaacgcgaa gacgagcccg agccggagca cgaagacgag ccggagaagg acgacgagcg
      841 gcgcgccgag ggagacgacg acgcggacga cgacaagggg atcctcacgc gcatcaagga
      901 gcgaggacag gtcttctagg gaaggagccg tgatgttcgg acgcaagaag ggactcgacc
      961 gggcgcgagg agtgctcgac gacgcaggtt ccggagcacg caaggcgctc agctcgaaga
     1021 aacccaacaa ggtcgactcg gtgaccgcgg ccgcgctgtt gctcggcttc gccaactggg
     1081 tctcggtcag cctcttcaac ttcgacctcg tccaggcagt cgcgaagaag aagtccgtgc
     1141 cgggccgtgc cgcctacggc ttcctaggcc tcagcgcgct ctacgcgtcg atccgcggca
     1201 cgcggggggt gaagtagatg gcgatggcac agcggtacgc cgcgcaacag gggatgagcc
     1261 ggggcgggcc gagcggcctt gccgacgtcc tcgaggtgct cctcgacaag ggtctggtga
     1321 tcgacgcgtt cgtgcgcgtc tcgctcgtcg gcatcgagct gctcaccatc gacgccagga
     1381 tcgtggtcgc cagcgtcgac acgtacctgc gctttgcgga agcggtcaac cgtctcgacc
     1441 tggtgcaggc gaacaactcg cagacgctcg gggacctcgt gtccgggtcg aaggagaagg
     1501 gcgcgaagca cgtcacgaag ggcgcgctcg aagggatccg cgagaagctc cccggtgggg
     1561 gcaaggaaga gcgcgagcac gaagaggcga ggtcctaggc gatgcccgag acccctctct
     1621 acgtctacgg gatcgtccgc tccgacgcga cgttgccggg cggcgccccg cgcggcgtcg
     1681 ccggcggctc cgtccgcctc ctggaggacg gctcgctcgc ggcggtcgtc accgacctgc
     1741 cgtcctcgtc gtacgaggcc ggccgcggcg acctgctcgc ccactcggag gtcctccagc
     1801 acgcgttcgc gtcggccgac gtcctgccga tggcgttcgg taccgcgttc gtgtcgcagg
     1861 gcgagctcgc cgagaccttc ctgcgcccga accacgacgc gctcgtcgcg atgctcgact
     1921 ccgtcggagg gatggcggag gtccaggtcc gcgccgacta cgagcaggac gcgatcgcac
     1981 gggacatcgc ggagtccgac cgctcggtcc gcaagctcca ggcacgtgcc cgtgagcgcg
     2041 gcgacgtcga gaccaagatc gagctgggac ggcgcttcgc ggccacgctc gagaacaaga
     2101 ggtacgcgga cgcacgggct ctcgtcgacg ctctgtcgaa ggccgcgcgc gacgtctcgc
     2161 tcggcgaggc tcccggagag tttggcctgc tgcgcgcgtc gttcctggtc ccccgggatg
     2221 gcctcgatcg gttcgactcc gccgccgaca cggccctcgc ttcgctcggg ggccgcgcga
     2281 ccctgcggtg cgtcgggccg ctcccgccct acagcttcgt cgacgcgtcg gcgctggtgg
     2341 tggcctgatg ggcctcttct cgaacgtcct cctgctccca ctggcgccag tgcgcggcgt
     2401 gctgtgggtt gcgcaggtgc tggccgacgt cgcgagcaag gagctgaacg acccgcagac
     2461 cctgcgggcg aggctgcgtg aggccgagga cgcccaccgc cgcggcgaga tctcggcgga
     2521 agacctcgac cgcatcgagg acgccgtctt ctcgcagctc gtcgcattgc agcaacgggg
     2581 aggtgtctcg tgagcgaacg cctttctgcc ggagaggcga tccgcgcagc ccgtgacgtc
     2641 gtcgtcgagc tgaccgggaa gcaggtcgaa ggcatctcgg ggatcaaccg ctcggacggt
     2701 gggtgggccg tctcgctgga cgtggtcgag ctcgcacgga tcccgtcctc cacggacctc
     2761 atcgcgacgt acgaggtctg cctcgacgac gacgggggcc tggtcgacat ggaacggcgc
     2821 cgccgctaca cccgcaacca gacggacgag gagtagccga tgcagctgca gaaccagccc
     2881 ggagcgcggc ccccagcatc cgccggcggc ggcgacctcg ccgacatcct cgagcgcgtc
     2941 ctcgacaagg ggatcgtcgt cgccggcgac gtcgcgatca acctcctcga catcgagctc
     3001 ctgacgatca agctgcgcct cctgatcgcg tcggccgaca ccgccaagaa gatggggatc
     3061 gactggtggg agagcgaccc gttcctgtcg ggcaaggccg gctccgagct tcccgagggc
     3121 aacggcaacg gcaagggcgg ggcgctcgag gagcggctcg accggatcga gaacgcactc
     3181 gcgcagctcg cgaagccgcc ggaggaacga tgactcccgg gctgtacgtc tacggaatcg
     3241 tcgccacggg ctccgttccg ggcccgctcc gggagcacgg cgtcggcgac ggcccgccgg
     3301 tcctcttcga cgtcgacggc ctgtccgcga tcgtgagcgg catcgacgtc tccgagtacg
     3361 agggagcggc gctcgaacgc aacgccgcga cgcccgaatg gctggagctg aaggtgcggg
     3421 gccacgaggc cgtggtcgaa ggcgtgctgg cgcacgcgac cgtcgtgccg atgaggttcg
     3481 gatcgatctt ctcgtcgacc gagagcctgc agaagatgct caccgagcac gccgacgcgt
     3541 tgcgcgaggc gctgacccgc gtcgaagccc ggaccgagtg gggcgtcaag gtccactgcg
     3601 accgatcgcg gctcgtgagc gcgctcgtgg gttcgtcgcc cgagccggac tccggacgcg
     3661 gctacctgat gcagaagaag gcgcggctgg aagccgatgc gcgcgccgcg gaggcggcgg
     3721 cggagatcgc cgccgaagcc cacgcggcgc tggcggccgt cgcatccgag tcggtggcgg
     3781 ccccgccgcg cggcgaggag atcctcaacg gcgcctacct cgtggacgag gccgggcgcg
     3841 acgagttcat gcgcaaggtc gaggagctgc aacgccggca cgagggcgcg ttcgccttcg
     3901 aggtcaccgg gccgtggccg ccgtacagct tcacgagcgc cgacgtaggg gggccgcgtc
     3961 cgtgagcgcc gacctccagc gccaggtgac gttgctggac ctcgtcgacc ggatcgtcga
     4021 caagggggcc gtggtccggg gtcaggtcgt gctcgccgtc gcggacgtcg agctcgtcaa
     4081 gctcgacctc agcttgttgc tcgcgtccgt cgagacgctg gagggccgat gagctcgccc
     4141 gtcccggccc tcgcggaggt cgtcggcgac ctcgaacggg tccgcatcca tgccgaccct
     4201 tcgaacgccg agcgcgggct cgcgcagctc gtgctcacga tcgtcgagct gctccgccag
     4261 ctcatggaac gccaggcgat acgccggatg gagtcggggt cgttgaccga cgacgagatc
     4321 gagcggatgg gcgagacgtt catgcagctc gagcggcgca tgaccgagct gcgggagcac
     4381 ttcggcctcg cgccggagga cctcaacctg gacctgggcc cactcgggaa gttgctgtga
     4441 cgaaaaggag gtcccaatga tcgagaagat cgcacgccgg gccggaggct tcgcctcgtc
     4501 gaccgcgctg cgctggatac gcaccggagc ggacgaggtc gccgagatcg ccccgacgcg
     4561 gaacggaagc cacaagggcg gcggacgcgc ggtcgccgga gcggcagccg gagccgtcgc
     4621 cctgatcacg tcgagcaagg tggcccgctc ggggatcgag cgagggctgc gggcgctcgc
     4681 cgatgccgtg cggacgtcgt cgtccacgag ccacgggaac gggaacggca acgggaacgg
     4741 caacggctcg aaggacctga cggcgaagac ccgcgccgag ctctacgagc tggcgaagaa
     4801 ggccgacctg ccgggccgct cgtcgatgtc gaaggacgat ctcgccaaag ccctcaaggg
     4861 ctgaacccgg aacagaaaag aggggcccga gctttcgggc ccctcttctc gttgcggatc
     4921 gtggctaggc ggtgcgggcg gctcccgtcc tggccgtagc ccccgcggtg ccgaagaaga
     4981 tcgccagcgc tgctgttgcg atgtgcaaga agttgtccgc gctgttgatg ttcaacaagt
     5041 cctcgatgag gatgccggcg aagccgagga tcgctagcag caggtaaacg atcccgacca
     5101 tgaggttgac cgacttcgcc gtctcgtggc ggctcgatcc gatcagcagc agcacgccga
     5161 caccgaggtg cgcgatgttg tggagcgggt tgatcgcgaa cacgatgagc ttctcgcccg
     5221 ggacctcgct cgcgaagttg tcgaaccccg tgacggcgaa ccccacgagc ccgacgacga
     5281 gatagatgac gccgaagacc agcgcgaaca gctgggcagg cgtcttgtca cccacagttg
     5341 tcgacacgga cctccccttt cctcaccctt cacatccgat taggtagtgg ccttccctgt
     5401 tcgggccccc ggcactcccc ggattgccta aaactccgac gaccggagtg gcgcccggtt
     5461 acacgggggg tgccagaccg accggtgcaa ccggtgcaac cggtgcacgc tgcaccgcaa
     5521 gcaccgcaag caccgcaagt accgcaaggc accgcacggt cgcgtggata agccggcgga
     5581 ggcggcgccc ccggagcgac gactaagaaa cggtgtggag gagcggagcg gggcgccgcc
     5641 ggatcagccg gacgcgggcg agcgcgccta caggtaaccg aggttgcgca acgactcctc
     5701 gatcgcggcc tcgtcctcgg cggagtacgg cgacgcctcg gcgcccgatc ccgcgagcgg
     5761 aagatccatc gcctcgacca ccggggccgg gccgtcgggc agccactcgg tcagcactcg
     5821 gccgtccacc tcggggacct tcagcccggc caggtggagc gccgtcggca tgatgtcgta
     5881 gaggctcgcc ttcaggccgc tcgccggctc gatgccaggc cccgcgatcc cgaagatccc
     5941 gtccatgtgg tggaagccgc gcggaagccc ccggtagtcg tcgaggaccc ggggcgagta
     6001 gaggccgtcg gacagctcgt acgagtactc gcggcagacc gggaacaggt cgggcgcgtc
     6061 gacggcctga gggcccgaca cgacctcctc gcggcggtag atgcggtcga gcacgggctt
     6121 gccgtcgtcg gggtcgacga gcgcggagaa gcgctcgacg atctcgtcgc gccccgcctc
     6181 gtactcggac tccgggacga tcccgtgcgg ctcgcgccct tgcaggttca cgtagatgcc
     6241 ctgctgcggg atgggggccg agaacgcctt cgtgcggctc cagtcgatcg acgactgcgc
     6301 ggcccccttc gccttctgcc acacggactt cggcagcacc ttcttcgcct tgcgagcgag
     6361 cgtgcgtaca ccggcggact tcgtcaccgc gccggcaccg ccgatcgaca gcagacccca
     6421 ctcgcgcagc gcgaggttga cgttgacgct cttgtccttc gggccgaagc cgtggtcgga
     6481 catcgtcacg acgaagccgt cgttgccggc ccacgcgagc atgtcgccga tcatcccgtc
     6541 catctcgtcg aagaactccc acgctcgctc gcgtatcggc gcggcctccg gacggtggaa
     6601 gtgctcctgg cgcgggtcga tgtacttgta gtgcacgtgc atcaggcggt ccggggcctc
     6661 gaggacgccg aacagcaggc cgaggtcgcc gtgctgctcc agcaggtgcg caagggccag
     6721 ccgcttcttg tcgaggttcg ccgagagccg ctcgatgatc gccgtggact tccagtcctg
     6781 gtcgtagtcg acctcgatgt cgatctcgta gcccggcacg acgccggcga tctcggccga
     6841 cagcgactcc ggatgcgtga agctctccgg cgtcttgccg ccgccctcgg gggtcagcat
     6901 accggagacg gcgaagccgt cgacggcggg ggccgggtac gtcatcggga tgttgaacag
     6961 gcccatccgc acgccctgcg cgttcgccgc gttccagatc gcaggtgcgg tgacgcggtc
     7021 gaggctcacg agacccttgc tcgactggac gttgcccagc gtgaacccga agatcccgtg
     7081 cttgccgggg ttcacgccgg tgaagatcga cgtccacccc ggcggcgtgt acggcggcac
     7141 cgtcgacgtc aggacgcccg aggcaccgcg gtcgagcagc cgcttgaaca ccggcatgtg
     7201 accggcctcg aacgccggct cgagcaccga gtacgtcatc ccgtcgagcc cgagcagcag
     7261 cacccttttc atgctggagc acctccgagg cggtgcttcg cgctcaccgc agccgcctcc
     7321 acgcggtcaa gaggatcgcg gcggtgacca tcgcgatgcc gaggccgagc gccggcacga
     7381 cgagccccgt gatcgggccg aggatgaaga ccaccgcctg cacgtcgggc atgccggggt
     7441 tgggcaacag gcgtctgcgc gccagcgcgc cggagagccg gcccgccacg cctcccggcg
     7501 agtccgcctt ctcggccccc gagatctcga tgaagtagta cgcgagcacc gcgtacacca
     7561 cgacccacag gacgtcggac ggctcggccg cgtcggacct ccacgcccac agagcgaggc
     7621 cgagcgacgc gct
//
LOCUS       DASQDO010000132         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_564007, whole genome shotgun sequence.
ACCESSION   DASQDO010000132 DASQDO010000000
VERSION     DASQDO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564007"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VEV43_04030"
     CDS             <1..461
                     /locus_tag="VEV43_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HYP22720.1"
                     /translation="GAATLDDRWRAALAGLHRLFVDLGLDEDAQARVARRMRDGYANE
                     FRADALLKRQIGDRYRTERRSLEPLLDAAAPPEALTRRSTAVREPIRQLRDLAAAGRL
                     TVTFEELAASLGHMHVNRVIRSAQRAHEMVMYDLLDRLYVARAARRRSRP"
     gene            496..2094
                     /locus_tag="VEV43_04035"
     CDS             496..2094
                     /locus_tag="VEV43_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22721.1"
                     /translation="MSKRVSVVAFVAVVMLQSLLPAAATDDPVQSLSVYDLNSSLVYV
                     GLDDAGTATAAWSEVGALTVARRPEGGKFVEPQTIATAHVSEASLDVAPNGKAVLAFS
                     GGMVGGELMVAVRRNARGDFGRPQVLVPEGDPSRVVEPEAKVSESGRAVVVWTSETTG
                     EPPSIMAALSDSAGSFGTPAAVATGPDVHNAQVDVDASGRAFAVWDHTEAGTPHEIVG
                     AAAGTTGGFGAPQVLETLEQGPGAPDVAVNASGAAMVAHVDFTSEGEGESRDKVEARY
                     GSVNGTFGASQNVTDTSVPTAASEVEVAIDDGGRAAVLSSLTVRSEYGLYASVSDPSG
                     SFAAGSLQAVSPHERVTGPGIPRRSYEIAAGGGEFTAFWVNDHDDGTLNETWTATTAN
                     GTFGSAHQLSPEVEEDSPDRVHGARNADGDVAGAWLLFVDGMKAQVTPVSSGPDPIFG
                     DDGDDTLTGTSAGEQIWAAKGNDSAKGGGGGDQIFGEQGRDTLVGGPGLDSFDGGPGR
                     DVCVLSSNAEKGRTKSCERIRVRR"
     gene            complement(2098..2577)
                     /locus_tag="VEV43_04040"
     CDS             complement(2098..2577)
                     /locus_tag="VEV43_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP22722.1"
                     /translation="MVDAIPDNYPQVSPYLHVDGAAEAIKFYEDVFGAAERMRMPDPS
                     GKIGHAELQLGDSVIMLADEYPDMGIRGPAAYGGSPVTISLYVEDVDEVFQKAIESGA
                     KALQELSNQFYGDRTGQFEDPWGHRWSVASRVEEVSPEEMQRRAAESMGGLGEMPED"
     gene            2658..3473
                     /locus_tag="VEV43_04045"
     CDS             2658..3473
                     /locus_tag="VEV43_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYP22723.1"
                     /translation="MGDVRDPARRAPSELAWDTIAALYHRTRRTPVVGPLMLRAIEAR
                     TNGARWSRARVRSGPLRGMRLDVDMRVQADVVVGAYERRLSRHVDGLLRPGDLAFDVG
                     SHLGYFGILMAAAVGPAGRVVCFEPDPGLHDALEGNVSRNRALIPAEVAVARLAVGAE
                     RGTTSFEAGGHSTRGRLSDSGGVEVEVITLDDAFERFGAPRFVKVDVEGHELDVLEGG
                     PGAVASKQTSFGIEIHSDELGRACRALLEKAGYSCRFITEAGRAETYLLAEPR"
     gene            complement(3470..>3939)
                     /locus_tag="VEV43_04050"
     CDS             complement(3470..>3939)
                     /locus_tag="VEV43_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22724.1"
                     /translation="ETGRAQKLAQTFKAPEGAHKLASVSLPLSGAAKAVVKVYEVGER
                     PPFGTLLKEMVVTVGDASGEPASGWVRLPVRPAVAMKAGHRYSLVLKTDRKRDQLWTS
                     HANDRYRGGQSWCFCPVWKNQEIDFDTLRWQSAEDLDRPTDDLAFELRFWRKG"
BASE COUNT          560 a         1350 c         1469 g          560 t
ORIGIN      
        1 cgggggccgc gacgctcgac gaccgctggc gcgccgcgct cgccgggctg caccggctgt
       61 tcgtcgacct cggtctggac gaggacgcgc aggcgcgggt cgctcggcgg atgcgggacg
      121 gctacgcgaa cgaattccgc gccgacgcgc tgctcaagcg tcagatcggc gaccgctatc
      181 gcaccgagag gcgcagcttg gaaccgttgc tcgacgcggc cgcgcccccg gaagccctga
      241 cgcggcgctc gacggccgtg cgggagccca tccggcagct acgagacctg gccgcggccg
      301 gccgcctgac cgtcacgttc gaggagctgg cggcgagcct cggccacatg cacgtgaacc
      361 gggtgatccg ctcggcgcag cgcgcccacg agatggtgat gtacgacctg ctggaccggc
      421 tctacgtagc gagggccgca cggcgccgct cgcgtccgta ggatgggacc gtcgtcgaag
      481 ccggaggggg ctcgcatgtc gaagcgtgtg tcggtcgtcg cgttcgttgc cgtagtaatg
      541 ctccagtcgc ttctgccggc ggccgccacg gacgatccgg tgcagtcgct ctccgtgtac
      601 gacctgaact cgagcctggt ctacgtcggt ctcgacgacg ccggcacggc taccgcagcg
      661 tggtcggagg taggcgcgct gaccgtcgcg cgccggcccg agggcgggaa gttcgtcgag
      721 ccccagacga tcgcgacggc gcacgtctcc gaggcgtcgc tggacgtggc accgaacggc
      781 aaggcggtcc tcgcgttcag cggcgggatg gtcggcggcg agctgatggt cgccgtccgc
      841 cgcaacgcgc gcggcgactt cgggaggccg caggtgctgg tgcccgaggg tgacccgtcc
      901 cgggtcgtgg agccggaggc caaggtgtcg gagtccgggc gggcggtcgt cgtgtggacg
      961 agcgagacga cgggtgagcc gccgagcatc atggcggcgc tcagcgactc cgcgggcagc
     1021 ttcgggacgc cggccgccgt ggcgaccgga ccggacgtgc acaacgcgca ggtcgacgtc
     1081 gacgcctccg gacgggcgtt cgcggtgtgg gaccacaccg aggccgggac cccgcacgag
     1141 atcgtcggcg cagcggcggg aaccacggga gggttcgggg ccccgcaggt cctcgagacg
     1201 ctcgaacagg ggccgggcgc gcccgacgtc gcggtgaacg cgtcgggagc ggcgatggtc
     1261 gcgcacgtgg acttcacgtc cgaaggagag ggcgagtcgc gcgacaaggt cgaggcgcgc
     1321 tacggcagcg tgaacggcac gttcggcgcg tcgcagaacg tcaccgacac gtcggtgccg
     1381 accgcggcga gcgaggtcga ggtcgcgatc gacgacgggg gccgcgcggc agtgctctcg
     1441 tcgctcacgg tgcggtccga gtacggcctc tacgcctccg tctccgaccc gtcgggatcg
     1501 ttcgccgcgg ggtcgctgca ggcggtcagc ccgcacgaac gcgtgacggg ccccggtatc
     1561 ccgcgccggt cctacgagat cgccgccggc ggaggcgagt tcaccgcgtt ctgggtgaac
     1621 gaccacgacg acggcacgct gaacgagacc tggacggcga cgacggcgaa cggcaccttc
     1681 ggcagcgcgc accagctctc tcccgaggtg gaggaggact cacccgaccg cgtgcacggg
     1741 gcccggaacg ccgacggcga cgtcgcggga gcgtggctcc tcttcgtcga cgggatgaag
     1801 gctcaggtca cgcccgtgtc ctccggcccg gacccgatct tcggcgacga cggtgacgac
     1861 acgctgacgg gaacgtccgc cggcgagcag atctgggcgg caaagggcaa cgactccgcc
     1921 aagggcgggg gcgggggcga ccagatcttc ggcgagcagg gcagggacac gctcgtcgga
     1981 ggcccgggac tcgattcctt cgacggcggg ccgggccgcg acgtgtgcgt gctgtcgagc
     2041 aacgccgaga agggccgcac caagtcgtgc gagaggatcc gcgtgcggcg ctagaggtca
     2101 gtcctcgggc atctcgccga gtccgcccat cgactcggcg gcccggcgct gcatctcctc
     2161 cggagacact tcctccacgc ggctggccac gctccagcgg tggccccacg ggtcctcgaa
     2221 ctgtcccgtc cggtcgccgt agaactggtt gctcagctcc tgtagcgcct tcgcgccgga
     2281 ctcgatcgcc ttctggaaga cctcgtcgac gtcctcgacg tagaggctga tcgtcacggg
     2341 cgagccgccg tacgccgccg gcccccggat gcccatgtcg gggtactcgt cggcgagcat
     2401 tatcacggag tctccgagct ggagctcggc gtgcccgatc ttgccgctcg ggtccggcat
     2461 gcgcatgcgc tccgcggcgc cgaagacgtc ctcgtagaac ttgatcgcct cggcggcgcc
     2521 gtcgacgtgg aggtacgggg agacctgggg atagttgtcg gggatcgcat cgaccacttt
     2581 gtggacctcc tctggacgga cggtggactt tcgatcctaa gtggctagag tgcaccggcg
     2641 gccagtcgag aaggggggtg ggggacgtgc gggatccggc gcgccgcgcg ccctccgagc
     2701 tcgcgtggga cacgatcgcc gcgctctacc accggacgcg ccggacgccg gtcgtcggcc
     2761 ccctcatgct gcgcgcgatc gaggcgcgca ccaacggagc gcggtggtcc cgggcacgcg
     2821 tgaggtccgg gcccttgcgc gggatgaggc tcgacgtcga catgcgcgtc caggccgacg
     2881 tcgtcgtcgg cgcatacgag cgccgcctct cgcgtcacgt cgacggcctg ctgcggcccg
     2941 gcgacctcgc gttcgacgtc gggtcgcacc tcggctactt cgggatcctg atggcggcgg
     3001 cggtcgggcc ggcgggacgc gtcgtgtgct tcgagcccga ccccggcctc cacgacgccc
     3061 tcgagggcaa cgtctcacgc aaccgcgcgc tgataccggc cgaggtcgcg gtcgcgcgtc
     3121 tcgccgtggg agccgagcgc gggacgactt cgttcgaagc cgggggtcac agcacgcgtg
     3181 ggcggctcag cgactccggc ggcgtcgagg tcgaggtgat cacgctggac gacgccttcg
     3241 agcgattcgg cgcgccgcgc ttcgtgaagg tcgacgtcga gggtcacgag ctcgacgtcc
     3301 tcgaaggggg gccgggcgcg gtcgcgtcga agcagacctc gttcgggatc gagatccact
     3361 cggacgagct cggccgggcg tgccgggcgc tgctcgagaa ggcgggctat tcgtgccgct
     3421 tcataaccga ggccggccgt gccgagacgt acctgctggc ggagccgcgc tagcccttcc
     3481 gccagaagcg gagctcgaag gccaggtcgt ccgtcggccg gtcgaggtcc tctgcggact
     3541 gccaccgaag cgtgtcgaag tcgatctcct ggttcttcca caccgggcag aagcaccagg
     3601 actggccgcc gcgataacgg tcgttggcgt gcgacgtcca cagctggtcg cgcttccggt
     3661 cggtcttgag gacgaggctg taacggtgcc ccgccttcat cgcgaccgcc ggcctcacgg
     3721 gtagccgcac ccagccggag gcgggctcgc ccgaggcgtc gcccaccgtg acgaccatct
     3781 ccttcaggag agtcccgaac ggagggcgct cccccacttc gtagaccttc acgacagctt
     3841 tggcggcacc ggagagaggc agcgagacgg acgccagctt gtgggcgcct tccggagcct
     3901 tgaacgtctg cgcgagcttc tgcgcgcggc ccgtttccg
//
LOCUS       DASQDO010000133         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_197192, whole genome shotgun sequence.
ACCESSION   DASQDO010000133 DASQDO010000000
VERSION     DASQDO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197192"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VEV43_04055"
     CDS             complement(<1..305)
                     /locus_tag="VEV43_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22725.1"
                     /translation="MIVLPVEEQAAAPAVTSTEGDLRVVWTPRAVEDASSVVALLPRR
                     ARPPWVRPDVLVVGDELPSYLRAEKGFHLLDAQGVRDLLAAPARERRRGWSTRGHAP"
     gene            complement(655..1626)
                     /locus_tag="VEV43_04060"
     CDS             complement(655..1626)
                     /locus_tag="VEV43_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007468379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP22726.1"
                     /translation="MRVLVTGAAGFIGSHLVDRLLAEGEEVLAVDNLSSGRLSNLADA
                     RRSPTGKFTFQRVDVTSNALGELIKRQKPEVVFHLAAQVDVRKSVADPLYDATVNVLG
                     TLNVLQSASEVGARKVVFTSSGGCIYGEPAPERLPVTEEQVYWPEAMPESPYGVSKKI
                     AIDYLRYFKAVQDLDYTALALSNVYGPRQEPASEVGLEGQGVAIFSRKMLGGRPCTIY
                     GDGTQTRDFVYVDDVVSAFVAARDRGSGELVNVGSGTELSVNELYSKLAELTGSRFEP
                     VYAAARPGELQRIVVDPTKAGRELGWSPQVGLDDGLKQTVAWFRATS"
     gene            complement(1629..>1857)
                     /locus_tag="VEV43_04065"
     CDS             complement(1629..>1857)
                     /locus_tag="VEV43_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYP22727.1"
                     /translation="DDSHVAADARVSFSCLGYGVSVGSGARVTDSVLLPGVIVGEGAE
                     VHRSILGEGVKVAPGAVVEGRTAGDRETVEG"
BASE COUNT          271 a          651 c          647 g          288 t
ORIGIN      
        1 atcggcgcgt ggccgcgggt gctccacccg cgccgccgct cccgtgcggg tgcggcgagc
       61 aggtcgcgca cgccttgcgc gtccagcagg tggaagccct tctcggcacg gagataggac
      121 gggagctcgt cccccacgac caggacgtcc gggcgcaccc atgggggccg cgcccgccgc
      181 ggcagcagcg ccaccaccga agacgcgtcc tcgaccgccc ggggagtcca cacgaccctc
      241 aggtcgcctt cggtagaggt gaccgcgggg gccgcggcct gctcttcgac cgggaggacg
      301 atcactcggt gctcctccgg ggcggagtcc tgcgcttcgc gagggtggcg gccgttcgtg
      361 tggggtcgag tcgtggcgtg cgtttcggga ctcgaggtct cgaaagccaa ctttcctcct
      421 gcgcttagtc tcccgcgtcg cccggcgccg ccgagcttgc cttactctcc gccaccggac
      481 cgccgcggac cgaagtcgga cgatacctcg tccgggggtc tcggcccgcc cgattggggt
      541 gcccacagcc taagtcttcc agccaaatcc ttccactgtt ttttcttggg aattcaacga
      601 acttgggcgg cgcgatgccc ctttgatgac gttaacgccg aatttgggag ttggttagct
      661 ggtggcgcgg aaccacgcga cggtctgctt cagcccgtcg tcgaggccga cctgaggcga
      721 ccaccccagc tcccggcccg ctttcgtcgg gtccacgacg atccgctgga gctccccggg
      781 ccgcgctgct gcgtagaccg gctcgaagcg cgaccccgtc agctccgcga gcttcgagta
      841 gagctcgttg accgacagct cggtgccgct gccgacgttg acgagctcgc cgctgcccct
      901 gtcccgggca gcgacgaacg ccgagacgac gtcgtcgacg tagacgaagt cccgcgtctg
      961 ggtcccgtcg ccgtagatcg tgcagggccg gcccccgagc atcttgcggg agaagatcgc
     1021 cacgccctgg ccctcgagcc cgacctccga ggcgggctcc tggcgggggc cgtacacgtt
     1081 ggagagcgcg agcgcggtgt agtcgaggtc ctggaccgcc ttgaagtagc ggaggtagtc
     1141 gatcgcgatc ttcttcgaga cgccgtaggg cgactcgggc atggcctcgg gccagtagac
     1201 ctgctcctcg gtcacgggca gacgctccgg agcgggctcg ccgtagatgc agccgcccga
     1261 cgaggtgaac acgaccttgc gtgcgccgac ctcggacgcc gactgcagga cgttgagggt
     1321 gccgaggacg ttgacggtcg cgtcgtagag gggatcggcc acggacttgc gcacgtcgac
     1381 ctgggcggcg aggtggaaga cgacctcggg cttctggcgc tttatcagct cgccgagcgc
     1441 gttcgacgtg acgtcgacgc gctggaacgt gaacttgccg gtcggcgagc ggcgggcgtc
     1501 ggcgaggttc gacagacgtc cggacgacag gttgtcgacg gcgaggacct cctcgccctc
     1561 ggcgagcagc cggtcgacga ggtgcgagcc gatgaagccc gcagctccgg tgacgagaac
     1621 gcgcacgatt acccctccac tgtctctcga tcgccggcgg tccggccctc gaccaccgcg
     1681 ccgggcgcca ccttcacgcc ttccccgagg atcgagcggt ggacctcggc cccctcgccc
     1741 acgatgacgc cgggcaagag caccgaatcg gtgacgcggg ccccggaccc cacgctgacg
     1801 ccgtagccca ggcaggagaa ggatacccgg gcgtccgcgg ccacgtgcga gtcgtcg
//
LOCUS       DASQDO010000134         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_319361, whole genome shotgun sequence.
ACCESSION   DASQDO010000134 DASQDO010000000
VERSION     DASQDO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319361"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VEV43_04070"
     CDS             complement(<1..842)
                     /locus_tag="VEV43_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22728.1"
                     /translation="MKNKLCASLVGLALAATPLVPTVGAAASTTGFVRGVYGRDSSAT
                     GTSTLRATGFNAVTVQPWRDHLDALAAQGMGGVVWLFGYNNDTCSFDRDEAWVRQTVG
                     AIAGHPAITAYNVADEPNFRQCPDSPQEIAARARLVKSLDPANPTYVVVAAWDGREGF
                     PYQHFAGTTDIMGLDVYPCSHSMTSCKYADIDSAVEEAVKDGVTRYWAILQDFSDAWY
                     RQPTAAELERQFDHWARSGMEGYFLYHWNEGAVESRPDHLSVLRQQNSRTFGSGSAPT
                     PTPTP"
     regulatory      complement(856..934)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1076..>1581
                     /locus_tag="VEV43_04075"
     CDS             1076..>1581
                     /locus_tag="VEV43_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22729.1"
                     /translation="MRYAHFIDVLRADHEVRLALVRRESDARERPSPGDVDVTLSSRQ
                     VGANVARRALSMVPAQFSGFDDRGARAALRAAAGNADAFVALGARAGLYRDAFEGPAV
                     LDLQDSLVLNARAQWRGGRGVLRRLYGADNLLKAGAFERSLVERYDLVLVAGRADHGW
                     VRERHPRVR"
BASE COUNT          207 a          542 c          577 g          255 t
ORIGIN      
        1 ggggtcgggg tcggggtcgg ggccgagccg gagccgaacg tccgcgagtt ctgctgccgc
       61 aggaccgaca ggtgatcggg ccgcgactcc acggcgccct cgttccagtg gtagaggaag
      121 tagccctcca tcccggaacg cgcccagtgg tcgaactgac gctcgagctc ggccgccgtc
      181 ggctgccggt accaggcgtc gctgaagtcc tggaggatcg cccagtagcg ggtgaccccg
      241 tccttcacgg cttcctcgac ggccgagtcg atgtcggcgt acttgcaaga ggtcatcgag
      301 tgcgagcagg ggtagacgtc gagccccatg atgtcggtgg tccccgcgaa gtgctggtag
      361 gggaatccct cgcggccgtc ccaggccgcg acgacgacgt acgtggggtt ggcggggtcg
      421 agggacttca ccagacgagc cctcgccgcg atctcctgag gggagtccgg gcactgacgg
      481 aagttgggct cgtccgcgac gttgtacgcg gtgatcgcgg ggtgtccggc gatcgcaccg
      541 accgtctgac ggacccacgc ttcgtcgcgg tcgaagctgc aggtgtcgtt gttgtagccg
      601 aagagccaca cgacgccgcc catgccctga gctgcgagag cgtcgaggtg gtcgcgccac
      661 ggctgcacgg tgacggcgtt gaagccggtc gctcgcaggg tggacgtccc ggtggcgctc
      721 gagtcgcgcc cgtagacgcc acggacgaac ccggtcgtgg atgccgccgc tcccacggtc
      781 ggtaccagcg gagtggctgc cagagcgagg ccgacaaggc tcgcgcagag cttgttcttc
      841 atatgcttct cccagttcgg cagctgggcc acccaccaca ggtgggcccc tggctttgcg
      901 tccctgcgtc gccgcaggtt tgccttttcg ttctggtggt gcacctatag gttcgtcagc
      961 cacccggggg acctgtagac cctcctccga ccttccggta gctttcgcgg ccatgtcccc
     1021 gcggctcctg gtctgcgccg acacccgacc ctggccgccg gaccggggcg atcggatgcg
     1081 ctacgcccat ttcatcgacg tgttgcgcgc cgatcacgag gtacggctgg cgctcgtccg
     1141 gcgcgagagc gacgcgcgcg agcggccctc gcccggcgac gtcgacgtca cgctgagcag
     1201 ccggcaggtg ggggcgaacg tcgcgcgccg cgcgctgtcg atggtgccgg cccagttctc
     1261 cgggttcgac gaccgcgggg cgcgggcggc cttgcgcgcg gctgccggga acgccgacgc
     1321 gttcgtcgcg ctgggagcgc gtgccggtct ctaccgcgac gccttcgagg ggcccgccgt
     1381 gctcgacctc caggacagcc tcgtcctcaa cgcccgcgcg cagtggcgcg ggggccgcgg
     1441 cgtgctgcga cggctgtacg gggccgacaa cctcctgaag gccggggcct tcgagcgatc
     1501 gttggtcgag cgctacgacc tcgtgctcgt cgccgggcgg gccgaccacg gctgggtccg
     1561 ggaacggcat ccccgcgtcc g
//
LOCUS       DASQDO010000135         3868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_564207, whole genome shotgun sequence.
ACCESSION   DASQDO010000135 DASQDO010000000
VERSION     DASQDO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564207"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..911)
                     /locus_tag="VEV43_04080"
     CDS             complement(138..911)
                     /locus_tag="VEV43_04080"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HYP22730.1"
                     /translation="MMDDPLVLAGESFSSRLIMGTGGASSLEALERALVASGAEIATV
                     ALRRLDPSARGSILDVLGRAGCKVLPNTAGCFTAHDAVTTAQLAREAFETDWIKLEVI
                     GDDKTLLPDPAELLVAAEQLVDLGFVVLPYTNDDPILARRLEDVGCAAVMPLGSPIGS
                     GMGLLNPYNLRLIRERANVPVILDAGVGTASDAALAMELGCDAVLCASAISRAEDPVA
                     MARAIRLGVEGGRLAYRAGRIPRRLHAEASTPEYGTAEF"
     gene            complement(908..1117)
                     /gene="thiS"
                     /locus_tag="VEV43_04085"
     CDS             complement(908..1117)
                     /gene="thiS"
                     /locus_tag="VEV43_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HYP22731.1"
                     /translation="MTAVVNGTERSVGSGTTVAGLISELGHDPAGRGIAAALNGEVVP
                     RAAWGTTAVSDGDRVEILTAIGGGC"
     gene            complement(1114..2232)
                     /gene="thiO"
                     /locus_tag="VEV43_04090"
     CDS             complement(1114..2232)
                     /gene="thiO"
                     /locus_tag="VEV43_04090"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HYP22732.1"
                     /translation="MGVSPDVVVVGGGAVGLGVAWRCATAGAKVRVFDSSGGRGATWA
                     AAGLLAPVTEVHYGEEPLLALNLESSRRYPTFVAELEEASGATTGYRQSGTLMVALDG
                     DESRALDDLYRFQTSLGLSVERLNGRETRAEEPGLAPNVRAGILVEGDHQVDNRALLD
                     ALRAACVAAGVEIAADTVKGVEVASDRVVAVVTASEGAVPCSTVVLAAGSWSGAVEGL
                     PPDAVPPVRPVKGQLLHLRGEVLARRNLRGVEAYLVPRSDGRLVVGATVEERGWDTTV
                     TAGAVYELLRAAYELLPGVAELELTETVAGLRPGSPDNAPLIGPTSVEGLLLATGHYR
                     NGVLLTPVTADAIASVVTGAGMPDWASHFLPTRFGAAA"
     gene            complement(2257..2742)
                     /locus_tag="VEV43_04095"
     CDS             complement(2257..2742)
                     /locus_tag="VEV43_04095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP22733.1"
                     /translation="MEIHFSLCLPRDEESVPVVRHICRDALAELGVARDCVGDVELAV
                     TEACTNVLKHAAGTGDEYEVTVAIAEDDCVIRVIDSGTGFDHEAVDRTAEVEQGAESG
                     RGVFLMAALVDDLKFTSKPEVGTVVHLEKKLRLDETSVIRRLQRAKQAAVQAADGVPT
                     V"
     gene            complement(2790..3821)
                     /locus_tag="VEV43_04100"
     CDS             complement(2790..3821)
                     /locus_tag="VEV43_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HYP22734.1"
                     /translation="MIDLRSDTVTKPTPEMRRAMAEAEVGDDVFGDDPTVNELQELAA
                     GMLGKEAGLFVPTGSMGNQVALGAVARPGDEVVCESGAHFLHYEGGSVAAHLGLVARP
                     LPGVNGVISQSQVAAAIRPGSEHNPRTAVVAIENTHNSAGGRVFPLETARAISATCRE
                     RGVAVHLDGARLFNAQAATGSPAAEWAACADTVTFCFSKGLGAPIGSMVVSTRERIAE
                     ARRLRKRLGGGMRQVGVIAAAARVALTGGVDRLVEDHANARRLADGLADLNPDAVDFA
                     AVETNMVYVNLGAFGMDGPQVMEEMRKEGVITLALSADAMRLVTHRDVSAEDVETALK
                     AFRKVFKAA"
BASE COUNT          572 a         1458 c         1303 g          535 t
ORIGIN      
        1 gatggcgccg gcgccctgca gcggctcggt cgtcaacggg tcctcgtagt ggacctcgtc
       61 gtggcacaac ggcgcgaacg cctccgggtc gcgtcccgac caggcgcgct cccagccgtc
      121 gaagagcgcg tcggcggtca gaactcggcg gtcccgtact cgggggtgga cgcctcggcg
      181 tgcagccggc gcgggatgcg gcccgcgcgg taggcgagcc ggccgccctc gacgccgagc
      241 cggatcgcgc gcgccatcgc caccgggtcc tccgcccgcg agatggccga cgcgcacagc
      301 acggcgtcgc agccgagctc catcgccagc gccgcgtccg acgcggtccc gacccccgcg
      361 tcgaggatca ccggcacgtt cgcgcgctcg cggatgagcc gcaggttgta cgggttgagc
      421 agccccatgc cggagccgat cggggagccg agcggcatca ccgccgcgca gccgacgtcc
      481 tccagccggc gcgcgaggat cgggtcgtcg ttcgtgtacg ggagcacgac gaagccgagg
      541 tcgacgagct gctccgccgc gacgaggagc tcggccgggt ccggcagcag cgtcttgtcg
      601 tcgccgatga cctcgagctt gatccagtcg gtctcgaacg cctcacgcgc gagctgggcc
      661 gtcgtcaccg cgtcgtgagc cgtgaagcag ccggcggtgt tcggcagcac cttgcagccg
      721 gcgcgcccga gcacgtccag gatcgagcct cgcgccgacg ggtcgaggcg ccgcaaggcg
      781 acggtcgcga tctcggcccc cgaggcgacg agcgcgcgct cgagggcctc gaggctcgac
      841 gcgccaccgg tgcccatgat caaacgagag gagaagctct cccccgccaa aaccagaggg
      901 tcgtccatca acaaccccct cctatcgcag tgaggatctc gacccggtca ccgtcgctca
      961 ccgccgttgt tccccacgcg gcccgcggga cgacctcgcc gttgagggcc gcggcgatgc
     1021 cccgccccgc cgggtcgtgc cccagctccg agatgagccc ggcgacggtc gtgccggagc
     1081 cgacggaccg ctcggtcccg ttcacgacgg cggtcatgcg gcggccccga accgcgtcgg
     1141 caggaaatgc gacgcccagt cgggcatccc cgcgccggtg acgaccgagg cgatagcgtc
     1201 cgcagtcacc ggcgtcagca agaccccgtt ccggtaatga ccagtagcca gcagcaaccc
     1261 ctcgacggag gtcggcccga tcagcggcgc gttgtcgggc gatcccggcc ggagccctgc
     1321 caccgtctcg gtcagctcga gctccgccac tccgggaagc agctcgtacg cggcccgcag
     1381 cagctcgtac accgcgccgg cggtcaccgt cgtgtcccag ccccgctcct cgaccgtcgc
     1441 gcccacgacc agacggccgt ccgaacgcgg gacgaggtac gcctcgactc cgcgcaggtt
     1501 gcgacgcgcc agcacctctc ccctcagatg caggagctgg cccttcaccg gccgcaccgg
     1561 cgggactgcg tcgggcggga ggccttcgac cgcccccgac cagctccccg cggcgagcac
     1621 gacggtcgag catgggaccg cgccttcgga cgcggtcacg actgcgacga cgcggtcgga
     1681 ggcgacctcc acccccttga cggtgtccgc agcgatctcg actccggcgg cgacgcaagc
     1741 cgcgcgcaac gcgtcgagca gcgcgcggtt gtcgacctgg tgatctcctt cgacgaggat
     1801 gccggcgcgc acgttcggcg cgagcccagg ctcctcagcc ctcgtctccc gcccgttgag
     1861 acgttccacg gagagcccga gcgaagtctg gaagcggtag aggtcgtcga gcgcgcggct
     1921 ctcgtccccg tcgagcgcga ccatcaacgt cccgctctgc cgatatcccg tggtggcgcc
     1981 ggacgcctct tcgagctcgg cgacgaaagt ggggtagcgc cggctggatt cgaggttcag
     2041 cgcgagcagc ggctcctcgc cgtagtggac ctcggtgacg ggggcgagca gtccggctgc
     2101 ggcccacgtc gcgccgcggc ccccgctgga gtcgaagaca cggaccttcg cgcccgcggt
     2161 agcgcacctc caggcgacgc ctaggccgac ggcgccgcct ccgacgacga cgacgtcagg
     2221 agatacaccc atgcgatcag tatcgcgggt gcaggatcag acggtgggaa cgccgtccgc
     2281 cgcctggacc gcggcctgct tcgcgcgctg cagccgcctg atcaccgacg tctcgtcgag
     2341 ccgcagcttc ttctcgaggt gcacgacggt gcctacctcc ggcttcgagg tgaacttgag
     2401 gtcgtcgacc agggccgcca tgaggaacac cccccggccg ctctcggcac cctgctcgac
     2461 ctctgcggtg cggtcgacgg cctcgtggtc gaacccggtg ccggagtcga tgacgcggat
     2521 cacgcagtcg tcctccgcga tcgcaaccgt cacctcgtac tcgtcacccg tgccggccgc
     2581 gtgcttcagg acgttcgtgc acgcctcggt gaccgcgagc tccacgtccc ccacgcaatc
     2641 tcgggcaacg ccgagctcag cgagggcgtc gcggcagatg tgccgcacaa ccgggacgct
     2701 ttcttcatct ctcggcaggc aaagggagaa atggatctcc atgcgagcgt tcttcccccg
     2761 ccccgcgctc gcaaacgcga gcaagcaggt tacgcggcct tgaagacctt ccggaaagcc
     2821 ttgagcgcgg tctcgacgtc ttcggcggag acgtccctgt gcgtcacgag ccgcatcgcg
     2881 tcggcagaca gtgcgagcgt gatcacgccc tccttgcgca tctcctccat cacctggggg
     2941 ccgtccatcc cgaacgcgcc gaggttcacg tagaccatgt tcgtctcgac cgcggcgaag
     3001 tcgacggcgt cggggttcag atccgcgaga ccgtctgcga gccggcgggc attggcgtgg
     3061 tcctcgacga gccggtcgac gccgcccgtc aacgcgacgc gcgccgccgc ggcgatcaca
     3121 ccgacctgac gcatcccgcc gccgagccgc ttgcgcaacc ggcgcgcctc cgcgatccgc
     3181 tcgcgcgtcg acacgaccat cgacccgatc ggagcgccga gccccttcga gaaacagaac
     3241 gtgacggtgt ccgcacatgc agcccactcc gccgccggcg agcccgtggc ggcctgcgcg
     3301 ttgaacaagc gcgcgccgtc gaggtgcacg gccacgccgc gctcccggca cgtcgccgag
     3361 atcgcccgcg cggtctcgag cgggaacacg cggcccccgg ccgagttgtg ggtgttctcg
     3421 atcgcgacga ccgcagtacg cgggttgtgc tcgctcccgg gtcggatcgc agcggcgacc
     3481 tgcgactgcg agatcacgcc gttgacgccg ggcaacggac gcgccaccag accgaggtgg
     3541 gcggcgacgg acccgccctc gtagtggagg aagtgggcac cgctctcgca cacgacctcg
     3601 tcacccggcc gtgcgaccgc gccgagcgct acctggttgc ccatcgagcc ggtcggcacg
     3661 aacagcccgg cttccttgcc gagcatcccg gccgcgagct cctgcagctc gttgaccgtc
     3721 gggtcgtcgc cgaagacgtc gtcgccgacc tcggcctctg ccattgcgcg ccgcatctcg
     3781 ggcgtgggct tcgtcaccgt gtcggatcgc aggtcgatca tccgaagaac acctccgctg
     3841 tctcgtaaag ggtggggtcg agcgtctt
//
LOCUS       DASQDO010000136         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_748217, whole genome shotgun sequence.
ACCESSION   DASQDO010000136 DASQDO010000000
VERSION     DASQDO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748217"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VEV43_04105"
     CDS             <1..626
                     /locus_tag="VEV43_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22735.1"
                     /translation="QVMFAVARSTEQTTSNVRISEEARLGLNRIVRDTREAGWISLSG
                     SSPTATHNSFTVKIDYNGDGSYSNPASGTAQGNYEVVTYAYDDTNDRITVTADGVGTE
                     TLVRGVDCVKNAAGTCKSDVFSFTSNRLEYDYEGVSGTTPDGVTTLQEINGIACPPHS
                     LTTLDNCNGALVDAELAQITSLNVAVRLTTSGRSTDYFAEAQLRNRR"
     gene            623..2125
                     /locus_tag="VEV43_04110"
     CDS             623..2125
                     /locus_tag="VEV43_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22736.1"
                     /translation="MTFLERLRCQRGVAMITVLFIGATLTVLTTTAAFVSVRGLRATA
                     ADARSARAMAAAEAGLERFMLDIKRGAIPIATMKTAGCDTPPIALAPGTLGPGTYNVQ
                     LTIYEPTQAKKVPDSPWTAANKNVSPCTPRSSPNLYAVTATGTQSSGTRVVRQVIRAI
                     PGKSKFPLGIYADKIDANGNPDLNNISVFTRGDIIGRAKMSLSGIDENYTMRDVYSQS
                     AFGGFSWSGGLNWDSNIPAAVHATGEIYLKGTNARGREHPPSPNCTANDTRNGGYVLQ
                     SEWDGSACSGCANNAALTSDTCGGLAGYPPTARFTQADLDRIAQVRNFTEVEYEALKS
                     TAQSSGIYCNFASTVNQCWKNGVAGAVDLNWQNGDLPAGTGSTLVAYFDFPSSVNTAG
                     NTITWGATWKAISGVMCNALNPADNRMLVAVVRNGNFHFTGNAQVTGAVLVPEGDFDS
                     TGGPILHGSVTSRTFRVRGNATFESSSCFAAGLPTTSSELTIEGWSEIDR"
     gene            2169..2795
                     /locus_tag="VEV43_04115"
     CDS             2169..2795
                     /locus_tag="VEV43_04115"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HYP22737.1"
                     /translation="MADEHAGIKRESDDDATFRAVLSDVVQAVEDEGLPYLAIGGLAT
                     ATYGRPRPTKDIDVFVKPEDAERCVKTLESGGFSTEDPKEDWLLKAYKDDVLVDVIFR
                     IHNSIYLDDDMIARGRRQELKGTTIKVVPPEDFIVMQACTHSEDTPHYWYNALTVIAS
                     SEIDWDYVIRRSSHGPRRVLSLLLYAQSNDLAVPDHVIRRIYDQIHGL"
BASE COUNT          541 a         1025 c          909 g          388 t
ORIGIN      
        1 tgcaggtcat gttcgcggtc gcgcgctcga ccgagcagac cacgtcgaac gtccgcatct
       61 cggaggaagc gcggctcggc ctcaaccgga tcgtccgcga cacccgcgag gcgggatgga
      121 tctcgctgtc cgggtcctcc cccaccgcga cgcacaactc gttcaccgtc aagatcgact
      181 acaacggcga cggctcctac agcaaccccg catcggggac cgcgcaaggc aactacgagg
      241 tcgttaccta tgcctacgac gacaccaacg atcgcatcac ggtgaccgcc gacggggtcg
      301 ggaccgagac cctcgtccgc ggggtcgact gcgtcaagaa cgcggccggc acctgcaaga
      361 gcgacgtctt ctccttcacc agcaaccgtc tcgagtacga ctacgaaggg gtctcgggca
      421 cgacacccga cggcgtcacc accttgcagg agatcaacgg tatagcgtgc cctccgcaca
      481 gcctcacgac gctggacaac tgcaacgggg cgctggtcga cgcggagctc gcgcagatca
      541 cgagcctgaa cgtcgccgtc aggctcacca cctcggggcg gtccacggac tacttcgcgg
      601 aagcccagct gaggaaccgc cgatgacgtt cctcgagagg ctgcggtgtc agcgcggcgt
      661 ggcgatgatc acggtgctct tcatcggcgc gacgctcacg gtgctcacga cgaccgctgc
      721 cttcgtcagc gtcagggggc tgcgcgcgac cgccgccgac gcgcggtcgg cgcgggccat
      781 ggcggcggcg gaggccgggc tcgagcggtt catgctcgac atcaagaggg gcgcgatccc
      841 gatcgcgacc atgaagacgg ccggctgcga cacgccaccg atcgccctgg cccccggaac
      901 gctcggcccg gggacgtaca atgtccagct caccatctac gagccgacgc aggccaagaa
      961 ggtcccggac agcccttgga ccgccgctaa caagaacgtc tcgccgtgca ccccccggtc
     1021 gagcccgaac ctctacgcgg tcacggcgac ggggacgcag agctccggga cgcgggtcgt
     1081 gcgccaggtc atccgcgcca taccggggaa gtccaagttc cccctcggga tctacgccga
     1141 caagatcgac gccaacggca accccgacct gaacaacatc agcgtgttca ccaggggtga
     1201 catcatcgga cgcgccaaga tgtccctgtc cggcatcgac gagaactaca cgatgcggga
     1261 cgtctacagc cagagcgcgt tcggcggctt cagctggtcc ggcggcctca actgggacag
     1321 caacatcccc gccgccgtcc acgcgaccgg cgagatctac ctcaaaggta cgaacgcgcg
     1381 cgggcgggag cacccgccgt cgccgaactg cacggcgaac gacacgagga acggcggcta
     1441 cgtcctgcaa agcgagtggg acggcagcgc ctgctccgga tgcgccaaca acgccgccct
     1501 gacctcggac acgtgtgggg gcctcgccgg atatccgccg acggcgaggt tcacgcaagc
     1561 ggacctcgac cgcatcgccc aggtcaggaa cttcacggag gtggaatacg aggcgctcaa
     1621 atcgacggcg caatcgagcg ggatctattg caacttcgcg tcgaccgtga accagtgctg
     1681 gaagaacgga gtcgccggag cggtcgacct gaactggcag aacggcgacc tccccgccgg
     1741 cacgggatct acgctcgtcg cctacttcga cttcccctcg tccgtgaaca ccgcgggcaa
     1801 caccatcacc tggggcgcca cctggaaggc gatctcgggg gtgatgtgca acgcgctgaa
     1861 ccctgcggac aaccgcatgc tcgtcgccgt cgtgcgcaac gggaacttcc acttcaccgg
     1921 caacgcccag gtgaccggcg cggtcctcgt gccggaaggg gacttcgact cgaccggtgg
     1981 cccgatcctc cacggctccg tcaccagccg cacgttccgc gtccgcggca acgcgacgtt
     2041 cgagtcctcg tcttgtttcg ccgccgggct gccgacgacc tcgagcgagc tcacgatcga
     2101 gggctggtcc gagatcgacc gctagcagcg gagttaggcg tcccggccga gcgggtagca
     2161 actcgcgcat ggccgacgag cacgcgggca tcaagcgaga gtcggacgac gacgcgacct
     2221 tccgcgccgt cctctccgac gtcgtccagg cggtcgagga cgaaggtctc ccctacctcg
     2281 ccatcggcgg cctcgcgacc gcgacctacg gcaggccgcg gccgaccaag gacatcgacg
     2341 tcttcgtcaa gcccgaggac gccgagcggt gcgtgaagac gctcgagagc gggggcttca
     2401 gcaccgagga ccccaaggag gactggctcc tcaaggccta caaggacgac gtcctcgtcg
     2461 acgtcatctt ccggatccac aactcgatct acctcgacga cgacatgatc gcgcgaggtc
     2521 gccggcagga gctgaagggc acgacgatca aggtggtccc gcccgaggac ttcatcgtca
     2581 tgcaggcgtg cacgcactcc gaggacacgc cccactactg gtacaacgcg ctcacggtga
     2641 tcgcgagctc ggagatcgac tgggactacg tcatccgccg gtcgtcgcac ggcccccggc
     2701 gcgtgctcag cctcctgctc tacgcgcaga gcaacgacct cgcggtcccc gaccacgtca
     2761 tccgccgcat ctacgaccag atccatggcc tctgagggcg ccggggagcc gcgcgagtac
     2821 accgaggcgc acatcgcgga ggccctcgcc gaggacccgc ggg
//
LOCUS       DASQDO010000137         5503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_13433, whole genome shotgun sequence.
ACCESSION   DASQDO010000137 DASQDO010000000
VERSION     DASQDO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13433"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..861
                     /locus_tag="VEV43_04120"
     CDS             295..861
                     /locus_tag="VEV43_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYP22738.1"
                     /translation="MAWSIDTAHTSVGFVARHLGLSKVRGQFAGFRGEVEGDPNDLTT
                     AKGWFEVDMASIESGNADRDAHLKSADFFDVEKWPTMRFESKAIEGTGGNYRVTGDLT
                     IKDQTKEVTLDYEHGGEVTDPFGNRKIGGTLTGTIKRSEWGLTWNVPLESGGWLVSDN
                     IKIEIDLQVAESQAAVEQEAQAEAEISA"
     gene            1244..1411
                     /locus_tag="VEV43_04125"
     CDS             1244..1411
                     /locus_tag="VEV43_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22739.1"
                     /translation="MDNSNIRELFIDELSQVTGGADPLERVKQIVADIKDGWETTQAC
                     CEEGQCCDYWS"
     gene            1482..3203
                     /locus_tag="VEV43_04130"
     CDS             1482..3203
                     /locus_tag="VEV43_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HYP22740.1"
                     /translation="MSATTNFQVPTDDPGVALLRLVRKQKLPFSSRFVAGAVGAHPQP
                     QSLLALVQVAPHVGLRVTAARVEPEGLDELDLPAVVHFSAGAGGFGVLERVTPSHVEV
                     WDSRHGRQRIARPAFLEGWSGIAALAERDDSQRKTDPDYRKQRVVEVLGGAAGRPDMA
                     GERVLAGIAGGIVALLAVFSIANVPAGARASAALVVLIALAGLCISAVLSYASADTTV
                     NADVPGCPRGKLVNCESVIKSEYSKLRGLPMADLGTAFFGAALGLAATAALYPVSGVP
                     WTALAYAFLAAAPAAVALIGAQVVMRRFCTMCLAVHALVLAGAAVAWRFAGGVPFSSA
                     WRGVALFVLYFFVVAFAAIPFFQRETRMRRLFEVQARVAGSPFATLAHLTTEQVTGLR
                     GAESGIRLPGPPSPHELVLFAHPTCKQCSVVLPEIAQLAASGATEAYVTILPRYGDGT
                     ERKLCEVLVAAGVAGGAQAFARAFAFAKKRFIGLMEEDFTGAIAAEAGLDRASLETGL
                     GPARRMIAQVDAVAEDRVEGTPAMFFDGRLYPYGAPVAHLAQLLHRHSDLLPARPGVA
                     EPEAARA"
     gene            3200..4141
                     /locus_tag="VEV43_04135"
     CDS             3200..4141
                     /locus_tag="VEV43_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22741.1"
                     /translation="MSGRPVVLVGSPRDAHVDALYESLTERGTDVVVADTLSFPDDYV
                     ISLGHEIDSITVAGRRVGRPAAVYIRDTYVHPLSYGVDADAEMDQDWRRTLVAFREKG
                     QMLVPLLTRWTEMGIPMYNPASRDWRNSKGFQIAALHQAGLPVPRTLWTNDPDAVKEF
                     AGGQRVVYKPVAGGAATRELGPEDLTDERLRKLKGAPVTFQELLEGDNFRVYCLDGKV
                     IAQIRVTSKEIDFRQNEEIVEETELSPEVQQQCLKAAEVIGMRWTGMDLRADTSGTLK
                     FLELNSSPMFLGFDARAGTKIRETLVARLAEHGEAAL"
     gene            4138..4920
                     /locus_tag="VEV43_04140"
     CDS             4138..4920
                     /locus_tag="VEV43_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HYP22742.1"
                     /translation="MKARIVLALAAAVLVGACSETDAQPLDDLQPTLGPGYVVVDEFP
                     HDPEAYTQGLDFQGARLFEGTGLNGRSSLRRVDLETGEVLRQVALADRFFGEGITVLG
                     KRIYQITWQNGRAFVYDKASFRRIRRFTYEGEGWGLTHDGRRLIMSNGSASIVFRDPK
                     TFEVLDEIEVTDGGEPVSMLNELEWVEGEIFANVWQTDDVVRIDPATGEVTGRLDLGA
                     LKQQQPNGEVTNGVAYMESQDRLFVTGKLWDALYEIELTELP"
     gene            5067..>5503
                     /locus_tag="VEV43_04145"
     CDS             5067..>5503
                     /locus_tag="VEV43_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22743.1"
                     /translation="METSTYPGTSTGGAVGEQVREGAQQVGEQVQQARDTVQETAQRA
                     MGQAQEQVGRARGQVREQVDQRLTQAGERAGTTAEDLRTVASELRRIDKDSSANVADQ
                     LAQRVEGVGRYLSSADGERILRDVEDFGRRQPWAVLAGGLALG"
BASE COUNT          869 a         1856 c         1974 g          804 t
ORIGIN      
        1 gcgacgatct cgctgtagcg gctcgcgccg gcgagcatcg accggatgat cgccccggtc
       61 caccgtctcc cgatgatctc catcgcgtgc gtatacgcgg ggcagaaggg ggcaagtacg
      121 tctttcgagg ggtcttcctt cacgtcccga ttctagccgg gaagctttct cttgacaagg
      181 acgttatgaa ggcatagcgt cttacgaaac ataaggactg tgaccgggac ggccggtcca
      241 aatggatggc agcggtcgcc tcggcgagcg gtccacaagg aaggggcacg gaagatggct
      301 tggagcatcg acaccgcaca cacgagcgtc gggttcgtcg cacggcacct ggggctctca
      361 aaggtccggg gccagttcgc cggcttccgc ggcgaggtcg agggcgaccc gaacgacctc
      421 acgaccgcca agggctggtt cgaggtcgac atggcctcga tcgagagcgg caacgccgac
      481 cgcgacgccc acctgaagag cgccgacttc ttcgacgtcg agaagtggcc gacgatgagg
      541 ttcgagagca aggccatcga ggggaccggc ggcaactacc gggtcaccgg cgacctcacg
      601 atcaaggacc agacgaagga ggtcacgctc gactacgagc acggtggcga ggtcaccgac
      661 cccttcggca accgcaagat cggcggcacg ctgaccggga cgatcaagcg cagcgagtgg
      721 gggctcacct ggaacgtccc gctcgagtcc ggcggctggc tcgtcagcga caacatcaag
      781 atcgagatcg acctgcaggt cgccgagagc caggcggccg tagagcagga ggcgcaggca
      841 gaggccgaga tctcggccta gcgacgcacc gaagtcaccg gcggcccccg catcatgcgg
      901 gggccgcggc gcgtcctgga cccccgaggc cgtcgccctg acgccccggt gtcacgctct
      961 ggacgaaatg gacaaaaccg ctaaactcct tccttgctct gtttgacggc ttaggaggac
     1021 gaaagtgaga aagaggatcg ccctcggcct gggtggtgtc cacgtggggc aacgagtgcg
     1081 gtctcggcta ggccgagact aggaggaacg gctcttccgc cgtagaaacc tcagccggga
     1141 gcgttcaacc agcggcttga cgcgggttga cccggcgctc caccggaacg agttcccgtt
     1201 tatccggcgt atcccgacta ggcgcgagga gaggaggaag aacgtggaca actcgaacat
     1261 ccgtgagctc ttcatcgacg agctgtcgca ggtcaccggc ggtgcggacc ccctcgagag
     1321 ggtgaagcag atcgtcgcgg acatcaagga cggctgggag accacgcagg cctgctgtga
     1381 ggaagggcag tgctgcgact actggagcta aggctctccg caagtcaccg tcacgccgcc
     1441 ccgaggggcg gcgtgacctt tttcagtaac cttcgaggga gttgagcgcc acgacgaact
     1501 tccaggtccc gaccgacgac cccggggtcg cgttgctccg gctcgtgcgg aagcagaagc
     1561 tcccgttctc gagccggttc gtcgccggcg cggtcggagc gcacccgcag ccacagtcgc
     1621 tgctcgcgct cgtccaggtc gcgccgcacg tcggcctgcg ggtgacggcc gcgcgggtcg
     1681 agcccgaggg tctcgacgag ctcgatctcc ccgcggtcgt ccacttctcc gccggagcgg
     1741 gcgggttcgg cgtgctcgag cgggtgacgc ccagccacgt cgaggtgtgg gacagccgcc
     1801 acggccgtca gcgcatcgcg cgccctgcct tcctcgaggg atggtcgggc atagctgcgc
     1861 ttgccgaacg cgacgactcg caacgcaaga ccgaccccga ctaccgcaag cagcgcgtcg
     1921 tcgaggttct gggtggggca gcggggcgtc ccgacatggc gggggagcgc gtcctcgccg
     1981 ggatcgcggg agggatcgtc gctctgctcg ccgtcttctc gattgcgaac gtcccggccg
     2041 gagcgcgcgc gtccgcagcg ctcgtcgtgc tgatagccct cgccggcctc tgcatctcgg
     2101 cggtcctgtc gtacgcgtcg gcggacacga cggtgaacgc ggacgtgccg ggatgcccgc
     2161 gcggcaagct cgtgaactgc gagagcgtca tcaagtccga gtactccaag ttgcgcgggc
     2221 tgccgatggc cgacctcggc acggccttct tcggcgcggc gctcgggctc gcggcgaccg
     2281 cggccctgta cccggtctcc ggcgtcccct ggaccgcgct cgcgtacgcg ttcctcgcag
     2341 cggcccccgc ggccgtggcg ttgatcgggg cccaggtcgt gatgcgacgg ttctgcacga
     2401 tgtgcctcgc cgtgcacgcg ctggtcctcg cgggggccgc ggtggcgtgg aggttcgccg
     2461 gcggcgtgcc gttctcgagc gcgtggcgcg gcgtcgccct cttcgtcctc tacttcttcg
     2521 tcgtggcgtt cgcggcgatc ccgttcttcc agcgcgagac gcgcatgcgc cgcctcttcg
     2581 aggtccaggc gcgcgtcgcc gggtcgccgt tcgcgacgct cgcgcacctg actaccgagc
     2641 aggtgaccgg tctgcgcggc gcggagtccg ggatccggct gccgggaccg ccgtcaccgc
     2701 acgagctcgt cctgttcgcg caccccacct gcaagcagtg ctcggtcgtc ctccccgaga
     2761 tcgcgcagct cgcggcatct ggcgcgaccg aggcctacgt cacgatcctg ccccgctacg
     2821 gcgacggcac cgagcggaag ctctgcgagg tcctggtcgc cgccggggtc gccggggggg
     2881 cccaggcgtt cgcccgcgcg ttcgcgttcg cgaagaagcg gttcatcggc ttgatggagg
     2941 aggacttcac cggggcgatc gccgcggagg cggggctcga tcgcgcgtcg ctcgagacgg
     3001 gcctcggccc cgcgcgccgg atgatcgcgc aggtcgacgc cgtcgccgag gaccgcgtcg
     3061 aggggacccc ggcgatgttc ttcgacggcc ggctgtaccc gtacggcgca ccggtcgcgc
     3121 acctcgcgca gctgctgcac cggcactcgg acctcctccc ggcgcggccc ggcgtcgccg
     3181 agcccgaggc ggcgcgggca tgagcggccg cccggtcgtc ctcgtggggt cgccgcgcga
     3241 cgcgcacgtc gacgccctgt acgagtctct gaccgagcgc ggcaccgacg tcgtcgtcgc
     3301 cgacacgctg tccttccccg acgactacgt gatctcgctc gggcacgaga tcgactcgat
     3361 cacggtggcg ggccggcggg tgggccgccc ggcggccgtg tacatccgcg acacctacgt
     3421 gcatccgctc tcgtacggcg tcgacgccga cgccgagatg gaccaggact ggcggaggac
     3481 gctggtcgcg ttccgcgaga aggggcagat gctcgtgccg ctgctcacgc gctggaccga
     3541 gatgggcatc cccatgtaca acccggcgtc acgcgactgg cgcaactcga agggtttcca
     3601 gatcgcggcg ctgcaccagg ccggactccc cgtgccacgg accctgtgga cgaacgaccc
     3661 cgacgccgtg aaggagttcg ccggagggca acgcgtcgtc tacaagccgg tcgcgggagg
     3721 cgcggccacg cgcgagctcg gtcccgagga cctcaccgac gagcgcctgc gcaagctcaa
     3781 gggcgcgccg gtgacgttcc aggagctgct cgaaggcgac aacttccgcg tgtactgcct
     3841 cgacgggaag gtgatcgcgc agatccgcgt gacgtcgaag gagatcgact tccgccagaa
     3901 cgaggagatc gtcgaggaga ccgagctgtc tccggaggtc cagcagcagt gcctcaaggc
     3961 tgcggaggtg atcgggatgc gctggaccgg gatggacctg cgcgcggata cgtcggggac
     4021 gctcaagttc ctcgagctga actcgtcgcc gatgttcctg ggcttcgacg cgagggccgg
     4081 gacgaagatc cgcgagacgc tcgtcgcgcg tctcgccgag cacggggagg ccgcgctgtg
     4141 aaggctcgca tcgtgctcgc gctcgcggcg gccgtgctgg tcggcgcgtg ctccgagacc
     4201 gacgcgcagc cgctcgacga cctgcagccg acgctcgggc cgggctacgt cgtcgtcgac
     4261 gagttcccgc acgacccgga ggcctacacg caggggctcg acttccaggg ggcccggctg
     4321 ttcgagggga ccggcctcaa cggccgctcg agcctgcggc gcgtcgatct cgagacgggc
     4381 gaggtgctgc ggcaggtcgc gctggccgac cgcttcttcg gcgaggggat cacggtcctc
     4441 ggcaagcgca tctaccagat cacgtggcag aacggccggg cgttcgtcta cgacaaggcg
     4501 tcgttccggc ggatccgccg cttcacatac gaaggcgagg gatgggggct cacgcacgac
     4561 ggacgccgtc tgatcatgag caacggctcc gcctcgatcg tcttccggga cccgaagacg
     4621 ttcgaggtgc tcgacgagat cgaggtgacc gacgggggcg agccggtctc gatgctgaac
     4681 gagctcgaat gggtcgaagg cgagatcttc gcgaacgtat ggcagaccga cgacgtcgtg
     4741 cgcatcgacc ccgccaccgg cgaggtgacc ggacggctcg acctcggcgc gctcaagcag
     4801 cagcagccga acggcgaggt cacgaacggc gtcgcgtaca tggagtccca ggaccggcta
     4861 ttcgtcaccg gcaagctttg ggacgcgctg tacgagatcg agctcaccga gctcccttag
     4921 cgtttccgcg tcgccgacgc gggtaggtcc atggctgcgg tcgagacgga gccgctgaga
     4981 acaagcccgg cggcccgcgc tgcatcccga aggttccttt cctttgtcgc gcgctcgtct
     5041 tcgggcacgg cagaggaggg agacgcatgg agacttctac ctatcccggg acgtccacgg
     5101 gcggagcggt cggcgagcag gtgcgcgagg gcgcgcagca ggtcggcgag caggtgcaac
     5161 aggcgcgcga caccgtccag gagaccgcgc agcgcgcgat gggccaggcg caggagcagg
     5221 tcggcagagc acgcggccag gtgcgcgagc aggtcgacca gcggctgacg caggccggtg
     5281 agcgtgcagg cacgactgcg gaagacctcc gcacggtcgc gagcgagctc cgccggatcg
     5341 acaaggactc gtcggcgaac gtcgccgacc agctcgcgca gcgcgtcgag ggcgtcggcc
     5401 gctacctgag ctccgcggac ggcgagagga tcctgcgcga cgtcgaggac ttcggacgcc
     5461 ggcagccgtg ggccgtgctc gcgggcgggc tcgcgctcgg ctt
//
LOCUS       DASQDO010000138         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_564310, whole genome shotgun sequence.
ACCESSION   DASQDO010000138 DASQDO010000000
VERSION     DASQDO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564310"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VEV43_04150"
     CDS             <1..253
                     /locus_tag="VEV43_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HYP22744.1"
                     /translation="LGVEVGSTDEVAAATGRLASEGLSTRAEDGVTCCYALQDKVWVN
                     DPSGAPWEIYTVLADAPGTSIRGDAACCTEGAEAGAACC"
     gene            complement(257..1411)
                     /locus_tag="VEV43_04155"
     CDS             complement(257..1411)
                     /locus_tag="VEV43_04155"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HYP22745.1"
                     /translation="MERPGPSAAHLEDVNPETTENGTVSRRTFGPAAVFCAWLGFLIC
                     LVYFAGALVNAPPPWQRVTSNWDTGWYLTIVNDGYQTAVNGETNLAFFPLYPLVIRLL
                     TKLGPDPVAAGVAFSLTCFLGALFLLRTLVAEKVSEQVARHTLLLLVFWPFSFFFGLV
                     YTESLFLLLVVGAFFFVHRRHWWLAAACAGLAGATRAVGIFVAVAVVLAYFEKEWSPT
                     VKKSATILGLAAAGLSGLGAFMVFLKVHTGDFLAFLEAQRFWPNRANGISGLRSIPTV
                     LQTRPSATWDFALVVIYMVPMVLFVVLCVYVLVKIDVVWGGFCLLALLAPVPTGSLAS
                     TNRYVLALFPCFVAAAKLLGDRAVVAAAASAGLLGLFAYHYTYHTGTFIG"
     gene            complement(1518..1694)
                     /locus_tag="VEV43_04160"
     CDS             complement(1518..1694)
                     /locus_tag="VEV43_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22746.1"
                     /translation="MRKRIVLLAASLGAASLIGFAPQAQASCTQLWIGGPCIETVVCR
                     VVDTVLPGPQHCVM"
     gene            complement(1821..1903)
                     /locus_tag="VEV43_04165"
     tRNA            complement(1821..1903)
                     /locus_tag="VEV43_04165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1868..1870),aa:Leu,seq:cag)
     gene            1982..>2658
                     /locus_tag="VEV43_04170"
     CDS             1982..>2658
                     /locus_tag="VEV43_04170"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HYP22747.1"
                     /translation="MGVAKSIEQRLEGLVEGFFTKVFRSGLQPVEVGRRILREMGNGK
                     TVSVNRIYAPNEFQVWMSADDYAKFSQMEAGLQREFSELVIEQAKESRWNLMGIPRIT
                     FHEEESMGRGELKVEASLTADPDSPSRVYTREPKEGDASSTRAVSSDTADRLGLEPSA
                     TRLVVIDDDGSDSESISITKDPVVIGRMSTNDVVLSDPNVSRRHAELRRAAGRWMLVV
                     LGSTNGTT"
BASE COUNT          487 a          885 c          924 g          362 t
ORIGIN      
        1 cctgggggtc gaggtgggat cgaccgacga ggtggccgcc gcgaccggac gcctcgcttc
       61 ggagggactc tcgactcgcg ccgaggacgg cgtcacgtgc tgctacgcgc tccaggacaa
      121 ggtctgggtg aacgacccgt cgggggcccc gtgggagatc tacacggtcc tggccgacgc
      181 gccggggacc tcgatccgcg gcgacgcggc gtgctgcacc gagggggccg aggccggcgc
      241 cgcctgctgc tagagctcag cctatgaacg tccccgtgtg atacgtgtag tgataggcga
      301 acaacccgag cagccccgcc gacgcggcgg ccgccaccac cgcacgatcg ccgagcagct
      361 tcgcggcggc cacgaagcag gggaacagcg cgaggacgta gcggttcgtg ctcgcgaggc
      421 tcccggtcgg cacgggcgcc agcagcgcta ggagacagaa gcctccccac acgacgtcga
      481 tcttcacgag gacgtacacg cagagcacga cgaacagcac catcggcacc atgtagatga
      541 cgacgagcgc gaagtcccac gtcgcgctcg gtctcgtctg caggaccgtc gggatcgacc
      601 tgagcccgga gatgccgttc gctcggttcg gccagaacct ctgcgcctcg aggaacgcga
      661 ggaagtctcc cgtgtgcacc ttcaggaaga ccatgaacgc gcccaggccg gagagcccgg
      721 ccgcggcgag gcccaggatc gtcgcggact tcttgacggt cgggctccac tccttctcga
      781 agtaggccag cacgaccgcg acggcgacga atatcccgac ggcacgcgtc gcccccgcga
      841 gtcccgcgca ggccgccgcg agccaccagt gccgtctgtg cacgaagaag aacgccccga
      901 cgacgagcag gaggaacagc gactcggtgt agacgaggcc gaagaagaag ctgaacggcc
      961 agaagacgag cagcagcagg gtgtggcggg cgacctgctc cgagaccttc tcggccacga
     1021 gcgtccgcaa caggaacagc gcgccgagga agcacgtgag cgagaacgct acgccggcgg
     1081 cgaccgggtc cgggccgagc ttcgtgagca gccggatcac gaggggatag agcgggaaga
     1141 aggcgaggtt cgtctcaccg ttgacggccg tctgatagcc gtcgttgacg atcgtgaggt
     1201 accagccggt gtcccagttc gacgtgaccc tctgccacgg gggcggcgcg ttcacgagcg
     1261 cgccggcgaa gtagacgagg cagatcaaga agccgagcca ggcgcagaag accgcagcgg
     1321 ggccgaacgt ccgtcgcgag accgtgccgt tctcggtggt ctctgggttc acgtcctcga
     1381 ggtgtgcggc agagggcccc ggccgctcca tgcgaggaac gctatccgat ggttcgtccc
     1441 gaaaagcgaa acggccctcc ccccggggcc cgtttcgctg tgtttcgaat ggttctcggt
     1501 tccggccgag ccggtcgcta catcacgcag tgctgggggc cgggcaggac ggtgtcgacg
     1561 acccggcaga cgacggtctc gatacagggc ccgccgatcc agagctgggt gcacgaggcc
     1621 tgggcctggg gtgcgaagcc gatgagactg gccgcgccga gagaagccgc cagcaggacg
     1681 atgcgcttac gcatgtggat gcctccttct gagcccgctt gccttgcaag agcgcattgt
     1741 gccgacgtgt tacgagagag tcaacaacgg ggttggccag atggggccgc ggtggacgcg
     1801 gtccggagag acacctttag tgggcgaggg gggatttgaa cccccacacg cttgcgcgca
     1861 ctggcacctg aagccagcgt gtctgccgtt ccaccactcg cccgggcgct cggggcctcg
     1921 ccccaagccc ctgcagccta gcaaggggtg acaacagacc gcgcgggcat aatcgtcgct
     1981 tatgggtgtc gccaagagca tcgagcaacg gctcgagggg ctcgtcgagg ggttcttcac
     2041 caaggtcttc cgatccgggc tccagcccgt cgaggtcggc cggcgcatcc tgcgcgagat
     2101 gggcaacggc aagaccgtct cggtcaaccg gatctacgcc cccaacgagt tccaggtctg
     2161 gatgagcgcc gacgactacg ccaagttctc ccagatggag gccgggctgc agcgggagtt
     2221 ctccgagctg gtcatcgagc aggccaagga gagccgctgg aacctcatgg gcatcccccg
     2281 catcaccttc cacgaggagg aatcgatggg ccggggcgag ctcaaggtcg aggcctcgct
     2341 caccgccgac cccgactcgc ccagccgcgt ctacacacgt gagccgaagg aaggggacgc
     2401 gagcagcacc cgcgcggtct cgtcggacac cgccgaccgg ctcgggctcg agccctccgc
     2461 gacgaggctg gtcgtcatcg acgacgacgg ctcggactcc gagtcgatct cgatcaccaa
     2521 ggaccccgtc gtcatcggac gcatgtcgac gaacgacgtc gtgctctcgg acccgaacgt
     2581 cagccgccgg cacgccgagc tccgccgcgc ggccgggcgg tggatgctcg tcgtcctcgg
     2641 ctctacgaac gggacgac
//
LOCUS       DASQDO010000139        13117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_381188, whole genome shotgun sequence.
ACCESSION   DASQDO010000139 DASQDO010000000
VERSION     DASQDO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381188"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VEV43_04175"
     CDS             complement(<1..418)
                     /locus_tag="VEV43_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22748.1"
                     /translation="MLLLVFLALAVVVITLDYRQGPGGPLQRVKDFSVTLVAPVQRGF
                     TTVFRPVGNFFSSIGELGSLRSENSELEENLEQAEADVREAEALVEENERLRALLDLE
                     QSWVVSERVTARVIARGPANLQWAAIIDKGRNDGIRP"
     gene            complement(626..2419)
                     /locus_tag="VEV43_04180"
     CDS             complement(626..2419)
                     /locus_tag="VEV43_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22749.1"
                     /translation="MSRVLAVVVSLMVGLLGVASPASADHTNPREPLSPTEEKPAPQA
                     VVDGEGEWEYIFGLPPNPGTDLEIFKLRRRLYMSSGTLGGAPQGHVGQRLVRLLNKKK
                     KVAPKWVADHGSAACTTNESVATGLQHDASIARRNGVTLVVDTTDAGGPQGRCHDTDG
                     GGLELIDVSKLHKTKKFAPREVHLTRHVGTSHNATVDATRPWIIYNSSSTFNNTSQAQ
                     IDVVDIRSCMKKRQTLEQRRKSCRPKVFRMQFEPGWTNNVDREGEPIEGFDSSCHDIV
                     ARPGRLYCAALSGTAIFDVRNIADKKTGAVKGKPLPCEVIEGTETGAMVTDCAALATP
                     DEGEAIPEATGWRFLGSTHHPGRDCGTGPEPGQPDRCNNNVHVPSREGVAVSHEAVPT
                     PDGKYMFVTDERGGGIVPGGASCAPEQENPYGNGGVHVYDISDPAKIEYAQTPDDQKA
                     VFIGSVQVPGPTFCTSHRIELVPGEQRFFISWYSQGTKVVDYFFDANGRVVFRETATW
                     VPEGTNQWAVQPFKIVKNPDGTKTYYLVSSDIGRGIDVFSWTGEPNPKGTPPPAEPAA
                     QPSPERRASALPLGLLGVLVLAGGAIRKRLR"
     gene            complement(2497..3528)
                     /locus_tag="VEV43_04185"
     CDS             complement(2497..3528)
                     /locus_tag="VEV43_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYP22750.1"
                     /translation="MDNMFQFIGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAINTKT
                     GAILAVGSEAKRMIGRTPAHIVAIRPLKDGVIADFDVTEKMLRYFIQKVHRRSFLAKP
                     RVVVCVPSGITGVEQRAVEEATISAGARSAYIIEEPMAAAIGAGLPVHEPTGNMVVDI
                     GGGTTEVAVVSLGGIVTSQSIRIGGDELDESIINYIKKEYSLMLGERTAEEIKMAIAS
                     AFPMPEEQQAEIRGRDLVTGLPKTIVVSAEEIRRAIEEPVNAIVDAVKNTLDKTPPEL
                     ASDIMDKGIVLAGGGSLLKGLDERLKHETGMPIHVAEDPLFAVAVGSGKCLEEFEALK
                     RVLISSTRH"
     gene            complement(3646..4053)
                     /gene="ndk"
                     /locus_tag="VEV43_04190"
     CDS             complement(3646..4053)
                     /gene="ndk"
                     /locus_tag="VEV43_04190"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYP22751.1"
                     /translation="MPDVQQTFIMVKPDGVRRKLVGEVVARIERKGYELREMKLFTID
                     EALAQKHYAEHTEKPFFGELVSFITSGPVVAMVVEGPDAIAGMRQIMGATNPIEAATG
                     SIRGDYATVIGENIVHGSDGPESAEREINLFFG"
     gene            complement(4089..5387)
                     /locus_tag="VEV43_04195"
     CDS             complement(4089..5387)
                     /locus_tag="VEV43_04195"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HYP22752.1"
                     /translation="MADVSYAEASAHLDALGIDAMKGLAPSLDRIEALCDALDNPQRQ
                     IPAIHVTGTNGKTSTARIAASLLGATGLTVGTFTSPHLTSVRERIALNGEPISEDEFG
                     DAFSHLAPYLETVEARLGEQLSFFEVMTAMFFLWAAEAPVDAAVVEVGLGGRWDATNV
                     VPASVAVLTNIGLDHTALLGDDRVKIAREKAGIIKHDAFVVTAERDPAVLDVISAEAD
                     GMMAPVAVIGREFQVRDNSVALGGRFLAIETSARLYQDLFLPLHGAHQGVNAATALEA
                     VTRFLPGQVLGEEVVAQGLAETVVPGRMETVKRVLLDVAHNPDGMSALVSSIAASFDV
                     VDSVFVVGILGDKDYKGMLLELARLNPRVIFTDAPNVRSVDPQELSAAALKLGLEDCR
                     VAADVPSAVDLAKDMAGDALVCVTGSHYVVGAARDHLLNE"
     gene            complement(5380..8001)
                     /locus_tag="VEV43_04200"
     CDS             complement(5380..8001)
                     /locus_tag="VEV43_04200"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HYP22753.1"
                     /translation="MTELPKAYQPEEIEKRWYEEWERVGAFRADPVTDKKPYVIVLPP
                     PNVTGALHMGHALNHSLQDALIRRARMQGYEALWVPGTDHAGIATQNVVERELRKDGL
                     DRHALGREAFVDRVWEWKREYGGRITQQMRELGESCDWERERFTMDEGLSRAVRTVFV
                     RWFEDGLVYRGLRVINWCPQCQTALSDIEVEHEDVAGELVTFRYDLSDGSGSISVATT
                     RIETMLGDTGVFVHPSDERYAGLVGKTVRHPFFPDRELPIVADEAVDPEFGTGAVKGT
                     PAHDPVDFEMSERAGTEKVNIFTETAHVNENGGRFEGMDRYTARREVLAALRELGLVE
                     EVERPYVHAVGHCYRSKTEIEPWLSEQWFVKMRPLAEPAIAAAQDGRVTFHPPRFAKA
                     YLNWMENLRDWCISRQLWWGHRIPVWYCDDCGATIAALEDPTECKCGSSALRQDPDVL
                     DTWFSSQLWPFSTLGWPDDTPDLKTFYPTSVMVTAYEIIYLWVARMIMSGMYFMDGQV
                     PFADVFIHGIVRDGEGKKMSKSIGNVIDPLDMIERYGADALRFSLAFIAVPGNDSNSS
                     EERIEGARNFANKLWNASRFVLMSLGDDRPEITGELLVEDRWILSRLDDTIEVVGREL
                     DAFNFSEALHALHAFVWSELCDWYVEMAKPRLREGGAGAAGSVLVHCLDRVLRLLHPV
                     MPFITEELWSKLRPGAGSVMTAPWPAPEGRRDTGAEDSVARFQDLVVALRRLKIEHDV
                     PQGRRVPVAVAAGDLAAGLEPLLDQVVSLARLESIELVDDLGPAGADPRTITPAGLEA
                     AMRLGETVDPALERARLERKRDELAAEVKRAESKLGNESFVAKAPAPVVEKERAKLDE
                     ARAAFAKVEGQLGALGG"
     gene            complement(7998..9584)
                     /locus_tag="VEV43_04205"
     CDS             complement(7998..9584)
                     /locus_tag="VEV43_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22754.1"
                     /translation="MDRPLHRTVVAVVAIASLFATACDEAAPEAERPAALDLVPANAS
                     SYFEVAVDPPESQRESLAEVARRFPGDVRRAFPRELPKRIEEALHGFGLDVGLAFPVK
                     FEDLGGWLGGSVAGFVVTEVVQDFSVLLFAVDGSEEDAIASFSETLKETSDETYRGVE
                     YVSGKAFNGSVAEVGVVDGYLVVAGEGGFAATVDASEGDDTLRTVPEFEQAVGAVADD
                     ALATFYVDVGSLRNEPTLEDQITREELRSAGLYGDGIVAASLSVHGDTLVVDASSGLD
                     KGGLLEPVLRGYAKEDELGDLPHGAWLAARVPKVRAVIGAAMKLTGARQKPGWKVFKR
                     GLKAARLRFGKDVLRWMGDGSFYLGGGSPRSLDAGLLVDSRDPRKTGEFVVALGRLLA
                     GSGLRVSRLPSVATAADFDVRVPPLPGPLHVSGARAFSITYGRTLHQALREDGFLDDA
                     DDFVDAKEALGEGYGATLYADVDAARAFSEGTVGVLMGVLPKPFAEGVEPFLDQGDYL
                     IHGVALEDDRILQRIVIGVR"
     gene            complement(9591..11177)
                     /locus_tag="VEV43_04210"
     CDS             complement(9591..11177)
                     /locus_tag="VEV43_04210"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HYP22755.1"
                     /translation="MRAKVVVGLIAAALVVGGGAFALTRFMAPAEDAAIEYVPSDAVG
                     YMNVFVRPSNDQKRALDSLLQKFPGIDSTDEAIEKLTDLLDEGLAEGGMSYEEDLEPW
                     LGDQVAAFLTPGGTPELPNFAFLVESKNDGAAGDFVEKLAEEDGVVLEDVTYEGETYQ
                     MEEDADEPIAVAILDGFLVGGTEDAIKDVIDTRAGDETLAGDADYVEATEPLEDDWIG
                     LFYFDFAGFLSAYGETAGYGPQEQAALEAFGLDQEGSQAAILYASSNSITFESSGGFG
                     LGGQFGELTELVAAEGILPDLPGGTWAAYGIPNFGALFDGLFDVFKEIPGFDREQIDA
                     MFYGQTGLRLQEDVLSWMEDAGLFVQGTSIRDVGGGLVIESNDPAKTARLLERLEELV
                     TQQGIETKPESAGDLEGFSVLLPGVPAPIYALGGDRLVVGYGDAALDAASGEGGTLRD
                     SEAFAAAQEAVGEDFDISFYVDVDGAQAFGEAVAAFSGASMETYEQDAKPYVDVLTHV
                     IAAAKMEGDTIVQKFVVGVE"
     gene            complement(11264..12541)
                     /gene="clpX"
                     /locus_tag="VEV43_04215"
     CDS             complement(11264..12541)
                     /gene="clpX"
                     /locus_tag="VEV43_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HYP22756.1"
                     /translation="MPKFGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAETPELKLEDLPKPKEIYEFLNDYIVGQEQAKKVLSVAVYNHYKRIQVGAHV
                     DGDVELAKSNIVLLGPTGCGKTLLAQTLARMLNVPFAIADATALTEAGYVGEDVENIL
                     LKLIQAADYDVKRAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPHQEFIQIDTTNVLFICGGAFAGLEKIIESRIGRKGIGFGADVRKMEE
                     KNLGDILEHVLPEDLLKYGLIPEFIGRLPVITNVHNLDKEALVEILTEPKNALIRQFK
                     KFFEFDGVELDFTEDALEAVAEQSMLRGTGARGLRAILDEVLLNVMYDLPSREDVGKC
                     VINREVVLERVNPTLVPRTKERTERPARGERSA"
     gene            complement(12557..13024)
                     /locus_tag="VEV43_04220"
     CDS             complement(12557..13024)
                     /locus_tag="VEV43_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYP22757.1"
                     /translation="MPPADLHLGGIPGSWCLRSPRSDSDCSAPDRPQISGPTSAAFPR
                     GCPPGFWCQGSAGYQGTAADIEIHAREILDVNQRMIEIISEHTGQTTEQVRKDLDRDR
                     FMSAEESRAYGIIDEVIASRTVAEKKGGALLQGEPARIANAEAEGAPVESPEA"
     gene            13018..>13117
                     /locus_tag="VEV43_04225"
     CDS             13018..>13117
                     /locus_tag="VEV43_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HYP22758.1"
                     /translation="MARIGESGDLLKCSFCGKSQKQVKKLIAGPGVY"
BASE COUNT         1967 a         4598 c         4366 g         2186 t
ORIGIN      
        1 ccggacggat gccgtcgttg cgtcccttgt cgatgatcgc ggcccactgg aggttcgcgg
       61 ggccccgcgc gatgacgcgg gcggtgacgc gctcgctgac gacccaggac tgctcgagat
      121 cgagaagcgc cctcagccgc tcgttctcct cgacgagcgc ctcggcctcg cgcacgtcgg
      181 cctccgcctg ctccaggttc tcctcgagct cggagttctc gctccggagg ctcccgagct
      241 caccgatcga ggagaagaag ttcccgaccg gccggaagac cgtcgtgaac ccgcgctgaa
      301 cgggggccac gagcgtgacc gagaaatcct tgacccgttg gagcgggccg cccgggcctt
      361 gcctgtaatc gagagtgatc acgacgacgg ccagggccaa gaagacgagc agcagcaagc
      421 gccctcgtcc gtttcggcga tacatacgtt gtcaaaacct cttcgcctga gcgagaaacc
      481 tgggggcggc ggcgattcgt gtccggccgc gatccggcgg gccgccctgc gtcgggccat
      541 ggtagcccgc atgggtgatt tcgccctaag tggtcgttga cctgcacttt tgtgccgcgc
      601 ccggcgcgtg cgagcgcgaa aaacgtcagc gcagacgctt cctgatcgcc cctccggcca
      661 gcaccaggac gccgaggagg ccgagcggca gcgccgaggc cctgcgttcg ggtgacggct
      721 gggcggcggg ctccgccggc ggaggcgtcc ccttcgggtt gggctcgccc gtccaggaga
      781 agacgtcgat gccccggccg atgtcggagg agaccaggta gtacgtcttc gtgccgtcgg
      841 ggttcttgac gatcttgaac ggctgcacgg cccactggtt cgtcccctcg gggacccagg
      901 tcgcggtctc gcggaagacc accctgccgt tggcgtcgaa gaagtagtcg acgaccttgg
      961 tcccctggct gtaccaggag atgaagaacc gctgctcgcc cgggacgagc tcgatgcgat
     1021 gcgacgtgca gaacgtcggg cccggcacct ggaccgaccc gatgaagacg gccttctggt
     1081 cgtccggggt ctgcgcgtat tcgatcttcg cagggtcgct gatgtcgtag acgtggacgc
     1141 cgccgttccc gtaggggttc tcctgctcgg gcgcgcacga cgcgccgccg gggacgatgc
     1201 cgccgccgcg ctcgtccgtg acgaacatgt acttcccgtc gggggtcggg accgcctcgt
     1261 gcgacaccgc cacgccttcg cgcgacggca cgtggacgtt gttgttgcac cggtcgggct
     1321 gacccggctc gggtcccgtc ccgcaatcgc ggcccggatg atgcgtgctt ccgaggaacc
     1381 tccacccggt cgcctccgga atggcctccc cctcgtcggg agtcgccaag gcggcgcagt
     1441 ccgtcaccat cgcgcccgtc tcggttcctt cgatgacctc gcacggcagc ggcttgccct
     1501 tcaccgcgcc ggtcttcttg tcggcgatgt tgcggacgtc gaagatcgcc gtgccgctga
     1561 gggccgcgca gtagaggcgc ccgggacgag ccacgatgtc gtgacacgac gagtcgaaac
     1621 cctcgatcgg ctccccctcg cggtcgacgt tgttggtcca cccgggttcg aactgcatcc
     1681 tgaacacctt cggccggcac gacttacgcc gctgctccag cgtctgccgc ttcttcatgc
     1741 aggagcggat gtcgacgacg tcgatctggg cctgggacgt gttgttgaag gtcgagctgc
     1801 tgttgtatat gatccacggc cgcgtcgcgt cgaccgtcgc gttgtgcgac gtcccgacgt
     1861 gcctcgtcag atgcacttcc cgcggggcga acttcttggt cttgtggagc ttgctgacgt
     1921 cgatcagctc gaggcccccg ccgtcggtgt cgtggcatcg tccctgcggg ccgcctgcgt
     1981 cggtcgtgtc gacgaccagg gtgacgccgt tgcgcctcgc gatcgaggcg tcgtgctgca
     2041 gccccgtcgc gacggactcg ttcgtggtgc acgcggccga gccgtggtcg gcgacccact
     2101 tcggcgcgac cttcttcttc ttgttcagga gccggacgag gcgctgcccg acgtggccct
     2161 gcggcgctcc gcccagcgtg cccgacgaca tgtagagccg gcgcctgagc ttgaagatct
     2221 cgaggtcggt tccggggttc ggcggcaggc cgaagatgta ctcccactcg ccctcgccgt
     2281 ctacgacggc ctgcggggcg ggcttctcct ctgtcggcga cagcggctcg cgggggttcg
     2341 tatggtcggc ggaggccggc gaggccacgc ccaggagccc caccatcagg gatacgacga
     2401 cagctagaac cctcgacatc tcgtccgcct cccgcgactc gacctgctcg tcaggagtag
     2461 gtacccccgg cggggcgagt cgaaccgtag cgagcgttag tggcgggtcg aggagatcag
     2521 gacccgcttg agggcctcga actcctccag gcacttcccg gagcccaccg cgaccgcgaa
     2581 cagcgggtcc tcggcgacgt ggatcggcat gccggtctcg tgcttgaggc gctcgtccag
     2641 gcccttcagc agcgaccctc cgccggcgag gacgatgccc ttgtccatga tgtcgctcgc
     2701 gagctcgggc ggcgtcttgt cgagcgtgtt cttgaccgcg tcgacgatcg cgttgacggg
     2761 ctcctcgatc gcgcggcgga tctcctcggc cgagacgacg atggtcttcg ggagacccgt
     2821 gacgaggtcg cggccgcgga tctcggcctg ctgctcctcg ggcatcggga acgccgacgc
     2881 gatcgccatc ttgatctcct cggcggtgcg ctcgccgagc atcagcgagt actccttttt
     2941 gatgtagttg atgatcgact cgtcgagctc gtcgccgccg atgcggatcg actggctcgt
     3001 gacgatgccg cccagcgaga ccaccgcgac ctcggtcgtc ccgccaccga tgtcgacgac
     3061 catgttgccg gtcggctcgt gcacggggag gcccgcgccg atcgccgcgg ccatcggctc
     3121 ttcgatgatg tacgcgctgc gcgcgccggc ggagatcgtg gcctcttcga cagcgcgctg
     3181 ctcgacgccg gtgatgcccg acgggacgca gacgaccacg cgcggcttcg cgaggaacga
     3241 gcggcggtgc accttctgga tgaagtagcg gagcatcttt tcggtcacgt cgaagtcggc
     3301 gatgacgccg tccttcaggg gccggatcgc gacgatgtgc gcgggggtcc ggccgatcat
     3361 ccgtttggcc tccgagccga ccgcgaggat cgcgccggtc ttggtgttga tggcgacgac
     3421 cgagggctcg ttgagcacga tgccgcggcc gcggacgtag acgagagtgt tggcggtgcc
     3481 gaggtcgacc gccatgtcgc ggccgatgaa ctggaacatg ttgtccattg gtgtggctcc
     3541 tgggaagttt tgagattgag gccgggtgcg ctggtcaggg taacgcatgc gcgcaaaccc
     3601 gcccctggcg ggcgccgagg gtccagtcac gaggaccgga tagggctagc cgaagaagag
     3661 gttgatctcc cgctcggcgg actcggggcc gtccgaaccg tgcacgatgt tctcccctat
     3721 gaccgtcgca tagtcgcctc ggatcgagcc ggttgcggcc tcgatcgggt tcgtcgcccc
     3781 catgatctgg cgcatccccg cgatcgcgtc gggaccttcg accaccatcg ccacgaccgg
     3841 gcccgacgtg atgaacgaca cgagctcgcc gaagaacggc ttctcggtgt gctcggcgta
     3901 gtgcttctgc gcgagcgcct cgtcgatcgt gaacagcttc atctcgcgca gctcgtagcc
     3961 cttgcgctcg atacgcgcga ccacctcgcc cacgagcttc cggcgcacgc cgtcgggttt
     4021 caccatgatg aacgtctgct gtacgtcggg catcgttgcc tcctgaagtc gagtcggaag
     4081 gagcgaggct actcgttgag caaatggtcc cgcgctgccc ccacgacgta gtgggagccg
     4141 gtgacgcaca cgagcgcatc gcctgccatg tccttggcga ggtccactgc ggaagggacg
     4201 tccgctgcga cccggcagtc ctctagcccc agcttcagcg cggccgccga cagctcctgc
     4261 gggtcgaccg acctgacgtt cggcgcgtcc gtgaagatca cgcggggatt gagccgggcc
     4321 agctccaaca gcatgccctt gtagtccttg tcgcccagga tcccgacgac gaacacgctg
     4381 tccacgacgt cgaaggacgc ggcgatcgag ctcacgaggg cggacatccc gtcgggattg
     4441 tgcgcgacgt cgagcagcac gcgcttcacg gtctccatcc gccccggcac cacggtctcg
     4501 gcgagtccct gcgcgacgac ctcctcgccc agcacctggc ccggcaggaa cctcgtcacg
     4561 gcctcgagcg ccgtcgccgc gttcacgcct tggtgcgcgc cgtgcagcgg gaggaagagg
     4621 tcctggtaga ggcgggccga ggtctcgatc gccaggaagc ggcccccgag cgccacggag
     4681 ttgtcccgta cctggaactc gcgcccgatg accgccacgg gcgccatcat cccgtcggcc
     4741 tccgcggaga tgacgtcgag cacggcgggg tcgcgctcgg ctgtgacgac gaacgcgtcg
     4801 tgcttgatga tccccgcctt ctcgcgcgcg atcttcaccc ggtcgtcacc cagcaacgcg
     4861 gtgtggtcga ggccgatgtt cgtaagcacc gcgacgctgg ccgggacgac gttcgtggcg
     4921 tcccagcgtc cgccgaggcc gacctcgacg accgccgcgt ccaccggcgc ctcggccgcc
     4981 catagaaaga acatggcggt catcacctcg aagaacgaga gctgctcgcc gagccgcgcc
     5041 tcgacggtct cgaggtacgg cgcgagatgc gagaacgcgt caccgaactc gtcttcggag
     5101 atcggctcgc cgttgagcgc gatgcgctcg cggaccgacg tcagatgcgg ggacgtgaac
     5161 gtccccaccg tgagccccgt agccccgagc agcgacgccg cgatccgggc cgtcgacgtc
     5221 ttcccgttcg tgcccgtgac gtggatcgcc gggatctgcc gctgcgggtt gtcgagcgcg
     5281 tcgcacagcg cctcgatccg gtctaacgag ggagcgaggc ctttcatcgc gtcgatgccc
     5341 agcgcgtcaa ggtgcgccga cgcctccgcg tacgacacgt cagccaccta gagcgccgag
     5401 ctgaccctcg accttcgcga acgcggcccg cgcttcgtcg agcttcgccc gctccttctc
     5461 gacgacgggg gccggcgcct tggcgacgaa cgactcgttc cccagcttgc tctccgcgcg
     5521 cttgacctcc gcggcgagct cgtcgcgctt gcgctcgaga cgggcccgct cgagggccgg
     5581 gtcgaccgtc tcaccgagcc gcatcgcggc ctcgaggccg gccggcgtga tcgtgcgcgg
     5641 gtccgcaccg gcgggaccga ggtcgtcgac gagctcgatc gactcgagcc tcgccagcga
     5701 caccacctgg tccagcaagg gctcgagccc ggcggcgagg tcgcccgccg ccacggcgac
     5761 ggggacgcgg cggccctgcg ggacgtcgtg ctcgatcttg agccgccgga gtgcgacgac
     5821 gaggtcctgg aagcgcgcga cggagtcctc ggcccccgtg tcacgccggc cctccggggc
     5881 aggccacggc gccgtcatga ccgacccggc gccggggcgg agcttcgacc acagctcctc
     5941 ggtgatgaac ggcatgaccg ggtgcaggag ccgcagcacg cggtcgaggc agtgaaccag
     6001 cacgctcccg gcggcccccg ccccgccctc gcgcaggcgc ggcttcgcca tctcgacgta
     6061 ccagtcacac agctcgctcc acacgaaggc gtgcagggcg tgcagggcct cggagaagtt
     6121 gaaggcgtcg agctcgcgcc ccacgacctc gatcgtgtcg tcgaggcgcg acaggatcca
     6181 gcggtcctcg acgaggagct cgccggttat ctcgggccgg tcgtcgccca gcgacatcag
     6241 cacgaagcgc gacgcgttcc acagcttgtt ggcgaagttg cgggcgccct cgatccgctc
     6301 ctccgacgag ttcgagtcgt tgcccggcac cgctatgaac gcgagcgaga accgcagcgc
     6361 gtcggcgccg tagcgctcga tcatgtcgag ggggtcgatg acgttgccga tcgacttcga
     6421 catcttcttg ccctcgccgt cgcgcacgat gccgtggatg aagacgtcgg cgaacgggac
     6481 ctgcccgtcc atgaagtaca tccccgacat gatcatgcgg gcgacccaga ggtagatgat
     6541 ctcgtacgcc gtcaccatca ccgacgtcgg atagaacgtc ttgaggtcgg gcgtgtcgtc
     6601 cggccacccg agcgtcgaga acggccacag ctgcgacgag aaccacgtgt cgagcacgtc
     6661 ggggtcctgc cggagcgcgc tcgacccgca cttgcactcg gtcgggtcct ccagcgcggc
     6721 gatcgtggcg ccgcagtcgt cgcagtacca gacgggtatg cggtggcccc accacagctg
     6781 gcgcgagatg caccagtcgc gcaggttctc catccagttc aggtacgcct tggcgaagcg
     6841 aggcgggtgg aacgtgacgc ggccgtcctg cgcggccgcg atcgctggct cggcgagcgg
     6901 gcgcatcttg acgaaccact gctcggacag ccacggctcg atctctgtct tcgaccggta
     6961 gcagtgcccg accgcgtgga cgtacggccg ttcgacttct tcgacgaggc cgagctcgcg
     7021 cagggcggcc aggacctcgc ggcgcgccgt gtagcggtcc atgccctcga agcgcccgcc
     7081 gttctcgttg acgtgcgcgg tctcggtgaa gatgttgacc ttctcggtcc ccgcacgctc
     7141 ggacatctcg aagtcgacgg ggtcgtgcgc aggcgtgccc ttgaccgctc cggtcccgaa
     7201 ctcggggtcg acggcctcgt cggcgacgat cggcagctcg cggtcgggga agaacgggtg
     7261 ccgcacggtc ttgccgacca gccccgcgta gcgctcgtcg gacggatgga cgaacacccc
     7321 ggtgtcgccg agcatcgtct cgatgcgcgt cgtggcgacg gagatcgagc cggagccgtc
     7381 cgagaggtcg taccggaacg tgacgagctc gccggcgacg tcttcgtgct cgacctcgat
     7441 gtcggacagc gcggtctggc actgcgggca ccagttgatg acgcgcagcc cccggtagac
     7501 gagcccgtct tcgaaccacc tgacgaacac cgtccggacc gcgcgcgaca gcccctcgtc
     7561 catcgtgaac cgctcgcgct cccagtcgca cgactcgccg agctcgcgca tctgctgggt
     7621 gatccggccc ccgtactcgc gcttccactc ccacacgcgg tcgacgaacg cctcgcgtcc
     7681 gagcgcatgg cggtcgaggc cgtccttgcg cagctcgcgc tcgacgacgt tctgggtcgc
     7741 gatgccggcg tggtcggtcc ccggcaccca cagcgcctcg tatccctgca tccgcgcccg
     7801 gcggatcagc gcatcctgca gggagtggtt gagcgcatgg cccatgtgca gcgcgccggt
     7861 gacgttcggc ggcggcagca cgatcacgta gggcttcttg tccgtcaccg ggtccgcgcg
     7921 gaacgcgccg acgcgctccc actcctcgta ccagcgcttc tcgatctcct ccggctggta
     7981 tgccttcggc aactctgtca tctcaccccg atcacgatcc gttggagtat ccggtcgtcc
     8041 tcgagcgcga caccgtggat caggtagtcg ccctggtcga ggaacggctc gacgccctcg
     8101 gcgaacggct tcggaagcac gcccatgagc acaccgaccg taccttctga gaaagcgcgc
     8161 gccgcgtcga cgtccgcgta gagcgtcgcg ccgtacccct cgcccagcgc ttccttcgcg
     8221 tcgacgaagt cgtccgcgtc gtcgaggaaa ccgtcctcgc gtagggcctg gtgcagagtc
     8281 cggccgtacg tgatcgagaa cgcccttgcg cccgagacgt gcagcggccc gggcaacggc
     8341 ggcacccgta cgtcgaaatc ggccgccgtg gctacggacg ggagccgcga caccctcagc
     8401 ccggacccgg cgagcagccg cccgagcgcg accacgaact cccccgtctt gcgcgggtcc
     8461 ctcgagtcga ccagcagccc cgcgtctagg gatcgcggcg agcccccgcc gaggtagaac
     8521 gagccgtccc ccatccaccg caggacgtcc ttcccgaacc ggaggcgcgc ggccttcaag
     8581 ccgcgcttga agaccttcca gccgggtttc tgacgggccc ccgtcagctt catcgcggcc
     8641 ccgatgaccg cccggacctt cggcacgcgg gccgccagcc acgccccgtg cggcaggtcg
     8701 ccgagctcgt cttccttcgc gtagccgcgc aacacgggct cgagcaggcc gcccttgtcg
     8761 aggcccgacg acgcgtccac gacgagcgtg tcgccgtgca cggacagcga cgcggcgacg
     8821 atgccgtcgc cgtagaggcc cgccgatcga agctcctcac gggtgatctg gtcttcgagc
     8881 gtgggctcgt tgcgcaggga gcccacgtcg acgtagaagg tcgccagcgc gtcgtctgcg
     8941 accgcaccga ccgcctgctc gaactcgggg acggttctca gcgtgtcgtc gccttcggac
     9001 gcgtcgacgg tagccgcgaa ccctccttcg ccggcgacca cgagatagcc gtccacgacc
     9061 cccacctcgg cgacggagcc gttgaacgcc ttccccgaca cgtactcgac cccgcgatag
     9121 gtctcgtccg aggtctcctt cagcgtctcg gagaaggacg cgatcgcgtc ctcctcgctc
     9181 ccgtctacgg cgaacagcag cacgctgaag tcctggacga cctcggtgac gacgaacccg
     9241 gcgacggagc cgccgagcca tcccccgagg tcctcgaact tcaccgggaa cgcgagcccg
     9301 acgtccaagc cgaacccatg gagagcctcc tcgatccgct tcggaagctc cctcgggaag
     9361 gccctcctca cgtcgccggg gaaccgcctc gcgacctccg cgagcgactc gcgctgggac
     9421 tccggcgggt cgacggccac ctcgaagtac gacgaggcgt tcgcggggac gaggtcgagg
     9481 gccgccgggc gctcggcttc cggcgcggcc tcgtcacacg cggtggcgaa caacgaagcg
     9541 atagcgacga ccgcgacgac cgttcggtgc agcgggcgat ccattgccgg ctactcgacg
     9601 ccgacgacga acttctggac gatcgtgtcg ccctccatct tggccgcggc gatcacgtgc
     9661 gtcagcacgt cgacgtacgg cttcgcgtcc tgctcgtacg tctccatcga cgcgccgctg
     9721 aacgcggcca ccgcttcgcc gaacgcctgg gccccgtcga cgtcgacgta gaacgagatg
     9781 tcgaagtcct cccccaccgc ctcctgggcc gctgcgaacg cctcggagtc acgaagcgtg
     9841 ccgccctcgc ccgaggcggc gtcgagggcc gcgtcgccgt agccgaccac gagacgatcg
     9901 ccgcccagcg cgtagatggg agccgggacg ccggggagca ggaccgagaa gccttcgagg
     9961 tcacccgcgc tctcgggctt cgtctcgatc ccctgctggg tcacgagctc ctcgagcctc
    10021 tcgagcagcc gcgccgtctt ggccgggtcg ttcgactcga tcacgagacc gccgccgacg
    10081 tccctgatgc tcgtcccctg cacgaacagg cccgcgtcct ccatccacga cagcacgtcc
    10141 tcctgcagac ggagcccggt ctggccgtag aacatcgcgt cgatctgctc gcggtcgaag
    10201 cccggtatct ccttgaagac gtcgaacagc ccgtcgaaaa gcgcgccgaa gttaggaatt
    10261 ccgtacgcgg cccacgtgcc cccggggagg tcgggcagga tgccctcggc ggcgacgagc
    10321 tcggtgagct ctccgaactg gccgccgagg ccgaagccgc ccgagctctc gaacgtgatc
    10381 gagttcgagc tcgcgtagag gatcgccgcc tgcgagccct cttggtcgag cccgaacgcc
    10441 tcgagcgcgg cctgctcctg cggcccgtat ccggccgtct cgccgtatgc cgagaggaag
    10501 ccggcgaagt cgaagtagaa gaggccgatc cagtcgtcct ccagaggctc ggtcgcctcg
    10561 acgtagtccg cgtcgccggc cagggtctcg tcgccggcgc gcgtgtcgat cacgtccttg
    10621 atcgcgtcct cggtcccgcc gacgaggaac ccgtcgagga tcgcgaccgc gatcggctcg
    10681 tcggcgtcct cttccatctg gtaggtctcg ccctcgtagg tgacgtcttc gaggacgacg
    10741 ccgtcctcct cggcgagctt ctcgacgaag tccccagccg cgccgtcgtt cttgctctcg
    10801 acgaggaagg cgaagttcgg aagctcgggg gtcccgcccg gcgtgaggaa cgcggcgacc
    10861 tggtcaccga gccacggctc gaggtcttcc tcgtagctca tgccgccctc ggctagccct
    10921 tcgtcgagca ggtcggtgag cttctcgatc gcctcgtcgg tcgagtcgat ccccgggaac
    10981 ttctgcagca gcgagtccag cgcccgcttc tggtcgttcg agggccggac gaagacgttc
    11041 atgtacccga cggcgtccga agggacgtac tcgatcgccg cgtcctccgc gggcgccatg
    11101 aagcgcgtca acgcgaaggc gcctccgccc acgacgagtg cggcggcgat cagcccgacg
    11161 acgacctttg ctctcacgct ggacctcccc acgggtcgag acggcctcga acggcactaa
    11221 aggaatacaa cgaagcggag cgagcgcggt atcgggaaga agactaagcg gagcgctcgc
    11281 cgcgggccgg gcgctcggtg cgctccttgg tcctcggtac cagggtcggg ttgacccgct
    11341 cgaggacgac ctcgcggttg atcacgcact tgccgacgtc ctcgcggctc gggaggtcgt
    11401 acatgacgtt gagcaggacc tcatcgagta tggcgcggag ccctcgggcg ccggtccccc
    11461 gcagcatcga ctgctcggcc acggcctcga gcgcgtcctc cgtgaagtcg agctcgacgc
    11521 cgtcgaactc gaagaacttc ttgaactggc ggatcagcgc gttcttgggc tcggtgagga
    11581 tctcgaccag cgcctctttg tcgaggttgt ggacgttcgt gatgaccggg aggcggccga
    11641 tgaactcggg tatcagcccg tacttgagga ggtcctcggg gagcacgtgc tcgaggatgt
    11701 cgccgaggtt cttctcttcc atcttgcgga cgtcggcgcc gaagccgatg cccttgcggc
    11761 cgatgcggct ctcgatgatc ttctcgagcc cggcgaaggc gccgccgcag atgaacagca
    11821 cgttcgtcgt gtcgatctgg atgaactcct ggtgcgggtg cttgcggccg ccctgcggag
    11881 gcacgctcgc cgtagtcccc tcgaggatct tcagcagggc ctgctggacg ccctcgcccg
    11941 agacgtcgcg cgtgatcgac gggttctcgc tcttgcgcgc gatcttgtcg acctcgtcga
    12001 tgtagatgat cccggtctcg gcgcgcttca cgtcgtagtc ggcggcttgg atcagcttca
    12061 gcaggatgtt ctcgacgtcc tcgccgacgt agccggcctc ggtcaacgcg gtcgcgtccg
    12121 cgatcgcgaa cgggacgttg agcatccgcg cgagcgtctg cgcgagcagc gtcttgccgc
    12181 agccggtcgg cccgagcagc acgatgttcg acttcgcgag ctcgacgtcg ccgtcgacgt
    12241 gcgcgccgac ctggatgcgc ttgtagtggt tgtagaccgc gaccgagagg accttcttgg
    12301 cctgctcctg cccgacgatg tagtcgttca ggaactcgta gatctctttg ggcttgggga
    12361 ggtcttcgag cttgagctca ggggtctcgg cgagctcctc ttcgatgatc tcgttgcaga
    12421 ggtcgatgca ctcgtcgcag atgtacacgc ccgggccggc gatcaacttc ttgacctgct
    12481 tctgcgactt gccgcagaag gagcacttca gcaggtctcc gccgtcaccg aacttaggca
    12541 cctattcgct cccccgttac gcctctgggc tctcgaccgg agcaccctcg gcttcggcgt
    12601 ttgcgatcct ggccggctcc ccttggagga gcgcgccgcc ctttttctcg gcgacggtcc
    12661 ggctcgcgat cacctcgtcg ataataccgt aggccctcga ctcctcggca gacatgaagc
    12721 ggtcacggtc gaggtccttg cgcacctgct cggtcgtctg cccggtgtgc tcggagatga
    12781 tctcgatcat gcgctggttg acgtccagga tctcgcgggc gtggatctcg atgtcggccg
    12841 cggtcccctg atagcccgcc gatccctggc accagaagcc gggagggcag cctcgcggaa
    12901 aggccgcgga agtcggcccc gaaatctgag gccgatcagg ggcggagcag tcagaatcac
    12961 tacgcgggct cctgagacac caggagcctg gaatcccccc gaggtggagg tcagcaggtg
    13021 gcacgaatcg gtgagagcgg tgacctgctc aagtgctcgt tctgcgggaa gagccagaag
    13081 caggtcaaga agctcatcgc tggccccggg gtctaca
//
LOCUS       DASQDO010000140         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_626284, whole genome shotgun sequence.
ACCESSION   DASQDO010000140 DASQDO010000000
VERSION     DASQDO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626284"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1441
                     /locus_tag="VEV43_04230"
     CDS             179..1441
                     /locus_tag="VEV43_04230"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HYP22759.1"
                     /translation="MTVVTFVSVVVLIVAFFFKAQCLQPSDGRQLYQRLCYSDLPPLY
                     GLRGMAENVFPYVNGGLVDGSLFGGAIEYPVLTGLFMWLTGLPVNDGNQFFVVSAILL
                     TPAALATAYLLGRMTGRRALMWAAAPALVLYAFHNWDLLVVACAVAGVWWWSRRRPVA
                     AALWFGLGAAFKLYPAFFLAPLFFDELRRNGFRRALGSVAAGVGAFAAVNLPFFLAGR
                     EGWLATYRFHQLRGPNFDNVWALWFPSDTWLGDYGAITPERLNLVTGVLTGVFFLGAL
                     GFGWWRARRDETYPFLQVCAALLAAFLLWSKVHSPQYALWILPFFALLRLNVVWWALY
                     AIADAATYYGIFQWFYDQSLGRDFTAAKKAMIAGVWGRAVLLLLLFGLFLRSRPAVPE
                     FLSHPPANVPPTQGDAGLVTKGGATIHA"
     gene            1546..1836
                     /gene="rpsF"
                     /locus_tag="VEV43_04235"
     CDS             1546..1836
                     /gene="rpsF"
                     /locus_tag="VEV43_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYP22760.1"
                     /translation="MRDYEAMVIVDARLEEGDIQKAVDRFTKIITDAGAELGKVDRWG
                     TRRFAYEINHQHEGYYFGASFRAEEGLVEDLKRTLQISDEFIRGKIVRPEHV"
     gene            1862..2356
                     /gene="ssb"
                     /locus_tag="VEV43_04240"
     CDS             1862..2356
                     /gene="ssb"
                     /locus_tag="VEV43_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYP22761.1"
                     /translation="MASENVVTLVGNITDDPELRFTPSGLPVANFTVAVNRRVPKDGG
                     WEDKLEGFFRCSCWRDMAENVAESLTKGSRVMVVGRLQQRSWEDQDGNKRSAVEIQVD
                     EVGPSLRWATARPERSQRSGGGFGGGSPQGSQQGSQPPASSGGDSWGPPASVGGSVPD
                     EGGF"
     gene            2360..2626
                     /gene="rpsR"
                     /locus_tag="VEV43_04245"
     CDS             2360..2626
                     /gene="rpsR"
                     /locus_tag="VEV43_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HYP22762.1"
                     /translation="MARDRDRDNKRSSRGPQVRRGKKKFCQFCADKTTYVDYKDIALL
                     RKFMSDRGKIRARRVTGNCSQHQRLVATAVKNAREMALLPYSSR"
     gene            2642..>3039
                     /gene="rplI"
                     /locus_tag="VEV43_04250"
     CDS             2642..>3039
                     /gene="rplI"
                     /locus_tag="VEV43_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HYP22763.1"
                     /translation="MKVILSDDVDKLGKKGDVVVVADGYARNFLVPKGLAMTATKGSL
                     KQAELMQRARADREERAKEEAAKKVAHLGAAPVYISARAGEGGRLFGSVTSSAVARAI
                     LDQLAEDVDRRKVLLEDPIRSRGTHQVEVKL"
BASE COUNT          477 a         1020 c         1073 g          469 t
ORIGIN      
        1 cgatcatccc ggcgagcgag gagccgacgc cgcagcccga cgacgcgccc gccgagcgac
       61 gcgtgagcag cccggcggaa cgccgcggca accggctccc gcacgcgtac tggtaccgga
      121 ggagcgggcg atagcccgag aaggcgagga gtcgggcgag ggggcattgc ggcgggcggt
      181 gaccgtcgtg accttcgtct cggtcgtcgt cctgatcgtc gctttcttct tcaaggccca
      241 gtgcctgcag ccgagcgacg gccgccagct ctaccagcgt ctctgctact cggacctccc
      301 gccgctgtac gggctgcgcg ggatggccga gaacgtcttc ccctacgtga acggcggcct
      361 cgtcgacggg agcctgttcg ggggcgcgat cgagtacccg gtgctgacgg ggctgttcat
      421 gtggctcacg gggctcccgg tgaacgacgg caaccagttc ttcgtcgtct ccgcgatcct
      481 gctcacgccg gccgcgctcg cgacggcgta cctgctggga cggatgacgg gacggcgcgc
      541 tctgatgtgg gccgcggccc ccgccctcgt cctctacgcg ttccacaact gggacctgct
      601 cgtggtcgcg tgcgcggtcg caggcgtctg gtggtggagc agacgaagac ccgtcgcggc
      661 cgcgctgtgg ttcggcctcg gtgctgcgtt caagctgtat ccggcgttct tcctcgcgcc
      721 gctgttcttc gacgagctgc ggcgcaacgg gttccggcgc gcgctgggaa gcgtcgccgc
      781 gggtgtcggc gcgttcgcgg ccgtgaacct cccgttcttc ctcgcggggc gcgagggatg
      841 gctcgcgacc taccggttcc accagctgag ggggccgaac ttcgacaacg tctgggcgct
      901 gtggttcccg tccgacacct ggctaggcga ctacggagcg atcacgccgg agcggctgaa
      961 cctggtgacc ggcgtgctca ccggcgtctt ctttctcggc gcgctcgggt tcggctggtg
     1021 gcgcgcccgg cgtgacgaga cgtatccgtt cctccaggtg tgcgcggcgc tgctcgccgc
     1081 gttcctgctg tggagcaagg ttcactcgcc gcagtacgcg ctgtggatcc ttccgttctt
     1141 cgcgctgcta cggctgaacg tcgtgtggtg ggcgctgtac gcgatagcgg acgcggcgac
     1201 gtactacggg atcttccaat ggttctacga ccagtccctg ggccgcgact tcacggcggc
     1261 gaagaaggcg atgatcgccg gggtctgggg ccgggccgtg ctcctgctgc tgttgttcgg
     1321 gctcttcctg cggtcgcggc ccgccgtgcc ggagttcttg tcgcaccccc ccgctaacgt
     1381 cccgcctacg caaggagacg caggcttagt cacgaaggga ggtgctacga tccacgccta
     1441 gaggcggtcg tccggccgcc cacaaccccc tgctctaccc gccgctccaa gccgcgggcg
     1501 agcctcggag acgggtccca cccgctcgag cccataggag gacatatgcg cgactacgaa
     1561 gcgatggtca tcgtggacgc tcgcctggaa gagggcgaca tccagaaggc cgtcgaccgg
     1621 ttcaccaaga tcatcaccga cgccggagcc gagctcggga aggtcgaccg gtggggcacg
     1681 cgccgcttcg cttacgagat caaccaccag cacgagggtt actacttcgg agcgagcttc
     1741 cgcgccgaag aggggctcgt cgaggacctg aagcgaaccc tgcagatctc cgacgagttc
     1801 atccgcggaa agatcgtcag gcccgagcac gtctgacgga aaacatctaa ggaggccaga
     1861 gatggcatca gagaacgtcg taaccctcgt aggcaacatc accgacgacc ccgagctgcg
     1921 cttcacgccc tccgggcttc cggtcgcgaa cttcaccgtc gccgtcaacc gccgcgtccc
     1981 gaaggacggg ggctgggagg acaagctcga aggcttcttc cgctgctcgt gctggcgcga
     2041 catggccgag aacgtcgccg agtccctcac gaagggctcc cgcgtgatgg tcgtcggccg
     2101 cctccagcag cgctcgtggg aggaccagga cggcaacaag cgctcggccg tcgagatcca
     2161 ggtcgacgag gtcggcccgt cgctccgctg ggcgacggcg cgacccgaac gttcgcagcg
     2221 ttccggcggc ggcttcggcg ggggttcccc gcagggctcg cagcagggat cgcagccgcc
     2281 ggcctcgtcc ggcggcgaca gctggggacc gccggcttcg gtcggcgggt cagtccccga
     2341 cgaaggaggg ttctagacag tggcgcgtga cagggacagg gacaacaagc gcagctcgcg
     2401 cggcccgcag gtccgccgcg gcaagaagaa gttctgccag ttctgcgcgg acaagacgac
     2461 gtacgtcgac tacaaggaca tcgcgctgct gcgcaagttc atgagcgacc ggggcaagat
     2521 ccgcgcgcgc cgcgtgacgg gcaactgctc gcagcaccag aggctcgtcg cgaccgcggt
     2581 caagaacgcg cgcgagatgg cgttgctgcc gtactcgagc cggtagcggg agggagacga
     2641 cgtgaaggtc atcctcagtg acgacgtaga caagctcggc aagaagggcg acgtggtcgt
     2701 cgtggccgac gggtacgcgc gcaacttcct cgtgccgaag ggccttgcga tgacggcgac
     2761 gaagggctcg ctcaagcagg cggagctgat gcagcgtgcc cgcgccgacc gcgaggagcg
     2821 cgccaaggaa gaggccgcca agaaggtcgc ccacctgggc gcggcccccg tgtacatctc
     2881 ggcccgcgca ggcgaggggg gccggctgtt cggctccgtg acgagctccg ccgtcgcgcg
     2941 cgcgatcctc gaccagctcg cagaggacgt cgaccggcgc aaggtgctgc tcgaggaccc
     3001 gatccggagc cgcgggacgc accaggtcga ggtcaagct
//
LOCUS       DASQDO010000141         6152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_809802, whole genome shotgun sequence.
ACCESSION   DASQDO010000141 DASQDO010000000
VERSION     DASQDO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809802"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VEV43_04255"
     CDS             <1..1298
                     /locus_tag="VEV43_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYP22764.1"
                     /translation="AAPAAPRSVEEERPPNILLVITDDQRATASMQALPTVRRLFGRG
                     GRTYTNAFATTPLCCPSRASIVSGAYAHNHGVQTNDDGMDLDPDATVQRHLKDAGYRT
                     AVVGKYLNRWDPEVDPAHFDRFAVWLRGIYRHSSWNVDGKVRRPEGYTTGLMTGFAER
                     FLRSFEKDDDAPWYLWLGPNAPHSPYAAEHRYKGAKTKGWDGNPATRDRDVSDEPAFI
                     ERSQNSMEHARKIRRKQLRTLYSVDDQVAQVFDLLRELGEEEDTLAIFMSDNGFLWGE
                     HGIVGKRYAYTQSIQIPLMIRWAGHVDPGSVDDRLVANLDLAPTLLDAAGIPARRGFD
                     GASLFGDRTRDRLLIEQWRDEETYVPTMASLRTEDYQYVEYYADGGRVVARQYYDLAD
                     DPWQRRNLLGDASARNDPDVDALARRLREDRGCRGARCP"
     gene            1410..3728
                     /locus_tag="VEV43_04260"
     CDS             1410..3728
                     /locus_tag="VEV43_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22765.1"
                     /translation="METRVKEGRTALAITILLIVTLTVPTLIVSGGFRVASAQQSPSP
                     TGTAPTIELVNPSDYEGTPVLSDKDNNPNNEGPNDDPNDEKYHFNAWTRNAPNNATVE
                     FFIVENNNTHSLGAGTNRGGGVYDLYSAIPNTVPEGAATVRAVLFAGGELDRDEQAVA
                     VNQVDNTPETEEMVLEQGEYRATNQAETLEITYPENTGGVGFFGRPGQPRAASVEVRH
                     SRTGTAGDGAEEGGGPESIRVYYTVSHRGTDPDWKNCNTGADQADASDGIRCQLASGD
                     SPEWVTGIAAVTMDAEVLEQAPRNLNAEEAANAGDAHVAVGYNQVPTGVTIASPPGST
                     PTGCSPLIPVTVTDQIGRKIVGAPVDVHAVGPTDSLAVNTEDTEQGTAQTQPDNNDQP
                     ENHATEQGRKCSDGSPSAQNQGVHAEAGALPDRKHIESTDHTDGNGQFVYRLWSNAPG
                     GTTMTAFVDMDENDRHCASEAAGNGIVNWGNGGPQAPLPAGPEEQVCNQGTASASSTS
                     PTRSATTPSQSSTSASPTQSATTASPTATASRTGSPTVTPSQTSPTSTNTTSSPSTPG
                     STASSPQQSAVEVTLETSQPRKTFGKSFTLTGSVTSDNTACTDLINVRILRDVVGGQD
                     DFELWAQETTDANGAFSVSQKADVSATYIAEVLETATCDDATSAPQPVLVRAKVILRL
                     SRNRVPEGARVRFTIKTAPCPLTARDRVLLFRAIEGEFGKAGRKQTNQRCVATITRRV
                     NKTSVFQARWPKQTAELLAGKSRAKAVRVRND"
     gene            3888..>6152
                     /locus_tag="VEV43_04265"
     CDS             3888..>6152
                     /locus_tag="VEV43_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22766.1"
                     /translation="MTEKIGRGDRQRGVARITLVVLALVQVVILMATLSDGLFALAQP
                     NPSASATASRNASASPRQSPSQEASPTGPTIQWLNPSRYEPALVVSDKQDNDNAYHLV
                     AWVANRPSDAVVQFSYQGTQGTPNEVTICTATRVPTTNDTYECFWDLDETGTANGTYR
                     LRATLFSSSASPSGSPPPGGGPAAVDEDEETVLVEREAETVEIEYPVNGGPLGVYDPP
                     GAQRAGFVMDVQTSEGAGDVRAYYSESPPGTEPSWQNCQQTTTVFFSDGTRRIGCVLQ
                     QGDTAATNITAVAAVPIRAATPDVGPGLNCTPNLPFQTNCPGEPERADSGDAHRVFRY
                     TQDPATVELPPPSENRPNQQGNFSCQVLTSAVRDQNGRPIWRVNMDVHAQGPGDEVRF
                     GSAPGTHPFKAPDQGGHQTENTDTCGNAPAEQATPAQGRHSNVSGADPKHIESVEGSD
                     TSGRFTFAVRSPAREGTSTVTAWADEDDNDERQPSEPFDTTTVTFTRQTAGPSQSTSP
                     SQSTATATATATATQTATASNTSASATSPSGTSPATRRQTLNCEPESDTNPVGSSHTF
                     VCTVNDGSANVSGVRVDVEVSGANDADGDTRATPDMSCTTTGESGSCSITHGPGGFGT
                     TSEPGTSVYRAWIDADGDDRTIEADATEGVNEFGQPGQRNEPDDTDVIEKTWQRAPLD
                     CSPEQDGNPTGTSHTITCRATQGGGAAQQGTHVVAEASGANDPDSGDSPESPDFECRT
                     NQEGVCSFTHGPGGT"
BASE COUNT         1095 a         2263 c         2078 g          716 t
ORIGIN      
        1 gcgcggcccc cgccgcgccg cgctccgtcg aggaggagcg gccgcccaac atcctgctcg
       61 tgatcaccga cgaccagcgc gcgaccgcga gcatgcaggc gctccccacc gtgcgcaggc
      121 tcttcggccg cgggggccgg acctacacga acgcgttcgc caccactccc ctgtgctgcc
      181 cgtcgcgcgc gtcgatcgtc agcggcgcgt acgcgcacaa ccacggggtc cagacgaacg
      241 acgacggcat ggacctcgac ccggacgcga ccgtgcagcg gcacctgaag gacgcgggct
      301 accgcacggc cgtcgtcggc aagtacctga accgctggga ccccgaggtc gaccccgccc
      361 acttcgaccg gttcgcggtg tggctgcggg ggatctaccg ccactcgagc tggaacgtcg
      421 acggcaaggt ccgtcgcccc gagggctaca cgaccgggct gatgaccggg ttcgccgaac
      481 ggttcctgcg gtcgttcgag aaggacgacg acgcgccgtg gtacctgtgg ctcggcccca
      541 acgcgccgca ctccccctac gccgcggagc accgctacaa gggcgcgaag accaaaggtt
      601 gggacggcaa ccccgcgacg cgcgaccgcg acgtgtcgga cgagccggcg ttcatcgagc
      661 ggtcgcagaa ctcgatggag cacgctcgca agatccggcg caagcagctg cggacgctgt
      721 actcggtcga cgaccaggtc gcgcaggtgt tcgacctgct ccgggagctc ggcgaggaag
      781 aggacacgct cgcgatcttc atgtcggaca acgggttctt gtggggcgag cacggcatcg
      841 tcggcaagcg ctacgcgtac acgcagtcga tccagatccc gctcatgatc aggtgggccg
      901 gccacgtcga tccgggctcc gtcgacgacc gcctcgtggc gaacctcgac ctcgccccga
      961 cgctgctcga cgccgcgggg atccccgccc ggcgcggctt cgacggggcg tcgctgttcg
     1021 gcgaccgcac tcgcgaccgc ctgctgatcg agcagtggcg cgacgaggag acgtacgtcc
     1081 cgacgatggc gtcgctgcgg accgaggact accagtacgt cgagtactac gccgacggtg
     1141 ggcgtgtcgt cgcgcgccag tactacgacc tcgccgacga cccgtggcag cggcgcaacc
     1201 tgctgggcga cgcctccgca cgcaacgacc ccgacgtcga cgcgctcgcg cgacggctgc
     1261 gcgaggaccg cggatgccgg ggagcgcgct gtccctgaaa gcgaggcaac cccggcgccg
     1321 cgcccgtcgt tagccctcct aggggtagtc tcgcccggat catccatatt tctcgcaggc
     1381 ggatacgaag gttgcacgag ggggccgagt tggagaccag agtcaaggag gggcgcactg
     1441 ccctggcgat cacgatcctt ctgatcgtga cgctgaccgt gccgacgctc atcgtcagcg
     1501 gaggtttccg cgtggcgtcc gcacagcagt cgccgagccc gaccgggacc gcgccgacga
     1561 tcgagctggt gaacccgtcg gactacgaag ggacgccggt cctgtcggac aaggacaaca
     1621 acccgaacaa cgagggcccc aacgacgacc cgaacgacga gaagtaccac ttcaacgcgt
     1681 ggaccaggaa cgctccgaac aacgccaccg tcgagttctt catcgtcgag aacaacaaca
     1741 ctcacagcct cggcgccggc acgaacaggg gcgggggcgt ctacgacctg tactccgcga
     1801 tccccaacac cgttcccgaa ggcgccgcca ccgttcgcgc ggtcctgttc gcgggcggcg
     1861 agctcgaccg ggacgaacag gccgtcgcgg tgaaccaggt cgacaacact cccgagaccg
     1921 aagagatggt gctggagcag ggcgagtacc gcgcgacgaa ccaggcggag acgctcgaga
     1981 tcacctaccc cgagaacacg ggtggcgtcg gcttcttcgg gcgtcccggg cagccgcgcg
     2041 ccgcgagcgt cgaggtccgg cacagccgca cgggtacggc cggcgacggc gcggaagagg
     2101 gcggcggccc cgagagcatc cgcgtctact acacggtctc gcaccggggg accgaccccg
     2161 actggaagaa ctgcaacacc ggggcggatc aggcggatgc gtccgacggg atccgctgtc
     2221 agctcgcgtc gggagactcc cccgagtggg tcaccgggat cgctgccgtg accatggacg
     2281 ccgaggttct ggaacaggca ccgcgcaacc tgaacgccga ggaggccgcg aacgccggtg
     2341 acgcgcacgt cgcggtcggc tacaaccagg tcccgacggg cgtgacgatc gcgagccctc
     2401 cggggtccac ccccacgggg tgctcgcctc tgatccccgt aaccgtcacc gaccagatcg
     2461 gccgcaagat cgtcggggcc cccgtggacg tccacgccgt cggccccacc gacagcctcg
     2521 ccgtcaacac cgaggacacc gagcagggca cggcccagac ccagcccgac aacaacgacc
     2581 agcccgagaa ccacgcgacc gagcagggcc gcaagtgctc cgacggctcg ccgtcggcgc
     2641 agaaccaggg cgtccacgcg gaagccggcg cgctgcccga ccgcaagcac atcgagtcga
     2701 ccgaccacac cgacggcaac ggccagttcg tctaccggct gtggagcaac gcaccgggcg
     2761 gcacgacgat gaccgcgttc gtcgacatgg acgagaacga ccgccactgc gcgtcggagg
     2821 ctgccggcaa cgggatcgtc aactggggca acggaggccc ccaggcgccg ctgcccgccg
     2881 gtcccgagga gcaggtctgc aaccagggca cggcctcggc gagctcgacg tcgcccaccc
     2941 gctccgctac gacgccaagc cagtcgtcga cttcggcgtc gcccacccag tcggcgacca
     3001 cggcgagccc caccgcgacc gcgagccgta ccggcagccc cacggtgacg ccgagccaga
     3061 cgagcccgac gtccaccaac acgacttcgt cgccgtccac cccggggtcg accgcctcga
     3121 gcccacagca gtcggccgtc gaggtcacgc tcgagacgtc ccagccgcgc aagacgttcg
     3181 gcaagtcgtt cacgctcacc ggctcggtca cgtccgacaa caccgcctgc accgacctga
     3241 tcaacgtccg gatcctgcgg gacgtcgtgg gcggccagga cgacttcgag ctctgggcgc
     3301 aggagaccac cgacgccaac ggcgccttct cggtcagcca gaaggcggac gtgagcgcga
     3361 cctacatcgc cgaggtcctc gagaccgcga cgtgcgacga cgccacgtct gctccgcagc
     3421 cggtgctcgt ccgggcgaag gtcatcctca ggctctcccg caacagggtc cccgaggggg
     3481 cccgcgtccg cttcacgatc aagaccgccc cctgccccct caccgcgcgg gaccgggtcc
     3541 tgttgttccg ggccatcgag ggcgagttcg gcaaggccgg gcgcaagcag accaaccagc
     3601 gttgcgttgc gacgatcacc cgccgcgtaa acaagacctc ggtcttccag gcccggtggc
     3661 cgaagcagac cgccgagctc ctcgccggga agtctcgggc gaaggccgtc agggtcagga
     3721 acgactgacg caaccgaacg gcacggcagt cgttgtagtt agaggggcgg tctccggccg
     3781 cccctctgct ttttgctaac gccgtctccg gacgaggggt ctgggggcgg cgacggtaca
     3841 gttgcgcggt tgcgcgtcga cacgactggg cgtagggggg ctgcgggatg acagagaaga
     3901 tcggcagggg cgacaggcaa aggggtgtag cgcggatcac cctggtcgtc ctcgcattgg
     3961 tccaggtcgt catcctgatg gccacgcttt ccgacggcct cttcgcgctg gcccagccca
     4021 acccgagcgc gtcggcgacc gccagccgga acgcatcggc gagcccgcgc cagagcccct
     4081 cgcaggaagc ctcgcccacg gggccgacga tccaatggct caacccgtcg cgctacgagc
     4141 ccgcgctggt cgtctccgac aagcaggaca acgacaacgc ctaccacctg gtcgcctggg
     4201 tcgcgaaccg gccttccgac gcggtcgtcc agttctcgta ccaggggact caggggacgc
     4261 ccaacgaggt gacgatctgc acggcgacgc gcgtccccac taccaacgac acctacgagt
     4321 gcttctggga cctcgacgag accgggaccg cgaacgggac ctaccggctc cgcgccacgc
     4381 tgttctcgag ctcggcgagc ccgtccggct ctccgcctcc cggcggcggc cccgccgcgg
     4441 tggacgagga cgaggagacc gtcctggtcg agcgtgaggc cgagaccgtc gagatcgagt
     4501 acccggtcaa cggcggcccc ctcggcgtgt acgacccgcc cggggcacag cgcgccggat
     4561 tcgtcatgga cgtgcagacg agcgagggcg ccggcgacgt cagggcctac tactccgagt
     4621 cgccgccggg gaccgagccg agctggcaga actgccagca aacgaccacg gtcttcttct
     4681 ccgacggcac gcgccggatc ggctgcgtgc tccagcaagg cgacacggcc gcgacgaaca
     4741 tcaccgcggt cgccgcggtc ccgatccgcg cggcgacgcc ggacgtcggc cccggtctca
     4801 actgcacgcc gaaccttccc ttccagacga actgcccggg cgagcccgag cgcgccgact
     4861 cgggtgacgc ccaccgtgtc ttccgctaca cgcaggaccc cgcgacggtc gagctgccgc
     4921 ctccgagcga gaaccgtccg aaccagcagg gcaacttctc gtgtcaggtc ctgacgtcgg
     4981 cggtgcgcga ccagaacggc cggcccatct ggcgcgtgaa catggacgtc cacgcgcagg
     5041 ggccgggcga cgaggtgcgg ttcggcagcg cgccgggcac ccacccgttc aaggcgccgg
     5101 accagggcgg ccaccagacc gagaacacgg acacgtgcgg caacgcgccg gccgagcagg
     5161 cgactcccgc gcaggggcgg cactcgaacg tcagcggcgc cgaccccaag cacatcgagt
     5221 cggtcgaggg cagcgacacg tcgggtcggt tcacgttcgc ggtccgctcg cccgcccgcg
     5281 agggcacgag cacggtcacg gcgtgggccg acgaggacga caacgacgag cgccagccgt
     5341 ccgagccgtt cgacacgacg acggtgacgt tcacgaggca gacggccggc ccgtcgcagt
     5401 cgacgtcgcc cagccagagc accgcgacgg cgaccgcgac ggcaacggcg acgcagaccg
     5461 cgacggcatc gaacacgagc gcgtccgcga cgtctccttc ggggacctcg ccggccacca
     5521 gaaggcagac tctgaactgc gagcccgaga gcgacacgaa ccccgtcggc agcagccaca
     5581 cgttcgtgtg caccgtcaac gacgggtcgg cgaacgtctc gggagtgcgc gtcgacgtcg
     5641 aggtgagcgg cgcgaacgac gccgacggcg acacccgcgc gacgccggac atgtcctgca
     5701 cgaccacggg cgagtccggt agctgctcga tcacgcacgg ccccggaggc ttcgggacca
     5761 cgtccgagcc gggcacctcc gtctaccgcg cgtggatcga cgccgacggc gacgacagga
     5821 cgatcgaggc cgacgcgacc gagggcgtca acgagttcgg ccagcccggc cagcgcaacg
     5881 agcccgacga caccgacgtg atcgagaaga cgtggcagcg ggcaccgctc gactgctcgc
     5941 ccgagcagga cggcaacccg accgggacgt cgcacacgat cacgtgccgc gctacgcagg
     6001 gcggcggcgc ggcacagcag gggacccacg tcgtcgccga ggccagcggc gcgaacgacc
     6061 cggactccgg ggacagcccc gagtctcccg acttcgagtg ccggacgaac caggaaggcg
     6121 tgtgcagctt cacccacggc cccggcggca cg
//
LOCUS       DASQDO010000142         5345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1115613, whole genome shotgun sequence.
ACCESSION   DASQDO010000142 DASQDO010000000
VERSION     DASQDO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115613"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEV43_04270"
     CDS             <1..295
                     /locus_tag="VEV43_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22767.1"
                     /translation="MARPLSRPQLRSNQRKAFLWLRTYRPMHAPGCTTDLLVALLSAE
                     GGTEYPIVERTPEGSIVIHPQNIEPAVGETADRLVNAPGSIVTAVGTWVDCVV"
     gene            382..1458
                     /locus_tag="VEV43_04275"
     CDS             382..1458
                     /locus_tag="VEV43_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HYP22768.1"
                     /translation="MNRKAAVMRRKVTVTLLALGLVTAAAAPALACAGLVAPNGSVRL
                     TRTATLAAYVHNAEGVGVEHYVTSFQFQGGGAKFGSIVPLPDVPSKVTRGGDWTLQRL
                     QRETAPPQPVPASPTAEDGGGKAEELYETEIDGLLITILKGGSRAVGKWAKDEGFALT
                     PDAPEVLRFYAQRSKIFMAARFIPEKASKKELQEGDGIPIHLRIPTDDPWVPLRILAL
                     GKRPYADVNADVYLLTEDEPSMLPVASPAGTNRGMVLERSEEASDSLLDDLRSDKGMK
                     WVPEEMWLSYLRIQMNARNLSYDLAVDASGAGSPSEEDAYGKQEEEAGQTTESRQLGW
                     IVLPVAGMGAMQLIRSRRRRAQQT"
     gene            1455..1853
                     /locus_tag="VEV43_04280"
     CDS             1455..1853
                     /locus_tag="VEV43_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYP22769.1"
                     /translation="MRRAVAAALLVATAACSGGGDAGATREVEVEMRFSRFDPDDFEF
                     PAGTTVTFVVENRDPIDHEFLIGDEEVQRAHEEGTEPHHGAKPGEISVPAGETRTTTY
                     TFEEPGKLLVGCHLPTHWDYGMRGEITVTG"
     gene            complement(1843..2193)
                     /locus_tag="VEV43_04285"
     CDS             complement(1843..2193)
                     /locus_tag="VEV43_04285"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HYP22770.1"
                     /translation="MPTRVAERDLVIDLRDPPRPAPAETHPRRNTDFVLLVALNALNL
                     LDALLTYLVTSAGLAVEGNPLVGWMTLPGKVVFVAALSFVLWKLRPRALWIPVVGYSA
                     VVAYTIAGALWLSP"
     gene            2263..3759
                     /locus_tag="VEV43_04290"
     CDS             2263..3759
                     /locus_tag="VEV43_04290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HYP22771.1"
                     /translation="MRRPLAAFGAALVVLSLAPASAPARPAVAAGDDVTVIAVIDDGI
                     TPYHWDYLASKMLASPDVPLDRPPHEWLRGFPSPGAFADYEPLDLTLEEKNANESPEA
                     LHSKDLEEWDSVEQSTPAKVNYYWIPGTKIIGAVDFGTQKIYGPTNTHGTGASSVAVG
                     NIHGSCPECLLVFLDTGNFNQAQTEAALEWAESQPWIDAVSNSYGHSTVFTDNVYTGS
                     DTEAQKRATVRGQTVFFAAHNGHDRSFFVPNNTLLSSQPGPDWVVTVGAVDPASRGSF
                     TGHGKPADVSGVGLSYPSAYGSSTVGGEGFFSGTSNATPVVTGIYGRALYEARLALRG
                     PSRVQSKGVVARGSAVRCGNARPECELGDGVLTADELRTRLFEGAVHTEAGMTSGAGV
                     VAGPPLGEDEFLNEGHGTYLGLLDGEKTWLEEIARVVGPMLGRAKVLDRPEGEREWMV
                     VDSFCRQHLWGSWTGGYYVEGKTDLPGTSPDWPLRSLIETACPNVPPF"
     gene            3773..4735
                     /locus_tag="VEV43_04295"
     CDS             3773..4735
                     /locus_tag="VEV43_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HYP22772.1"
                     /translation="MKAIRVHQLGGPEVLVLDDLSDPVAHDGEVVVRLEAVGVNFVDV
                     YHRTGLYPRDLPVVPGSEGAGVVVEAAPETVFSAGDRVASARLAGAYAEAATVPAEAL
                     VPIPDGVPTELAAAVMVQGITAHYLLRSTCELDEGDWCVVHAAAGGVGQLLTQMARLQ
                     GVRVLGTVSTEAKAEVARSAGAEVVVVHGGRSFAEAAREVTDGAGVRAVFDGIGKDTF
                     DESLDCLAPRGHMVLYGQASGKVPPMDPLRLAKGSLFLTRPGIADYTATREELLWRAE
                     DVLSAVGAGTLSVHVHDRYPLAEASRAHADLQSRKTTGKLLLVP"
     gene            complement(4745..>5345)
                     /locus_tag="VEV43_04300"
     CDS             complement(4745..>5345)
                     /locus_tag="VEV43_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYP22773.1"
                     /translation="VAGSTDLMATSGIGIVGARDVGPEAARVAGDAARYLASQGLTVF
                     SGAAKGIDQVAMNAAFDAGGAVVGVLADSLERRLRDPDTRRAIHDEQVCLLTPYKPSM
                     GFTVANAMARNKLVYALARKTLVVQSDLETGGTWAGATEWLKRAPEDVLVWAGAGEGR
                     GNAALIHAGARAISDVEALVGEHPPANTHAGAQQLGFGV"
BASE COUNT          839 a         1840 c         1884 g          782 t
ORIGIN      
        1 gatggcccgg ccgctttcgc gtccgcagct ccgatcgaac cagcgaaaag ccttcctctg
       61 gttgcgcacc tatcgcccga tgcacgcgcc cggctgcacc accgatctcc tcgtcgcgtt
      121 gctctcggct gaagggggga ccgagtaccc gatcgtggag cggacccccg aggggtcgat
      181 cgtcatccat ccccaaaaca tcgagcctgc cgtgggcgaa acggccgacc gcttggtcaa
      241 tgcccccggt tctatcgtca ccgcggtcgg gacctgggtc gactgcgtcg tctgacgcaa
      301 aggcacgcgg agcgacctgg ccttttcggc gggaaccttc tacgctcact cgcacgtcta
      361 cgacggcgtg agaacgcgat cgtgaacaga aaggcggccg tcatgcgccg taaggtcacc
      421 gtcaccctgc tcgccctcgg gctcgtcacc gccgccgcgg cccccgcact cgcctgcgcc
      481 ggcctcgtcg cacccaacgg ctcggtccgg ctcacgcgga ccgcgacgct cgccgcgtat
      541 gtccacaacg ccgaaggggt gggcgtcgag cactacgtca cgtcgttcca gttccagggc
      601 ggcggcgcga agttcgggtc gatcgtcccg ctccccgacg tcccgtcgaa ggtgacgcgg
      661 ggcggggact ggaccctcca gcgcctccag cgtgagacgg cgccgcccca gcccgtcccg
      721 gcgtccccga ctgccgagga cggaggcggc aaggccgaag agctctacga gaccgagatc
      781 gacggcctgc tgatcacgat cctgaagggc gggagccgcg cggtcggcaa gtgggcgaag
      841 gacgagggct tcgcgctgac gccggacgcc cccgaggtcc tccgcttcta cgcgcagcgc
      901 agcaagatct tcatggccgc ccggttcatc ccggagaagg cctccaagaa ggagctccag
      961 gagggcgacg gcatcccgat ccacctgcgg atcccgaccg acgacccgtg ggtcccgctg
     1021 cggatcctcg cgctcggcaa gcggccgtac gcggacgtga acgccgacgt ctacctgctg
     1081 accgaggacg agccctcgat gctgccggtc gcgtcgcccg cggggacgaa ccgggggatg
     1141 gtgctcgagc gcagcgaaga ggcgtcggac tcgctgctcg acgacctccg ctcggacaag
     1201 gggatgaagt gggtccccga ggagatgtgg ctgagctatc tccgcatcca gatgaacgcc
     1261 cgcaacctct cctacgacct cgccgtcgac gccagcgggg ccggctcgcc gtcggaggag
     1321 gacgcgtacg gcaagcagga ggaggaagcc ggtcagacga ccgagtcccg tcagctcggc
     1381 tggatcgtgc tcccggttgc agggatgggc gcgatgcagc tgatcaggtc gcgccgccgg
     1441 cgcgcccagc agacgtgagg cgcgccgtcg ccgcggctct cctggtggcg acggcggcat
     1501 gctccggcgg cggtgacgcc ggggcgacgc gcgaggtcga ggtggagatg cgcttctcgc
     1561 gcttcgaccc cgacgacttc gagttccccg ccggcaccac cgtcacgttc gtcgtcgaga
     1621 accgcgaccc gatcgaccac gagttcctca tcggcgacga ggaggtccag cgagcccacg
     1681 aggaagggac cgagccccac cacggcgcga agccaggcga gatctcggtt cccgcgggcg
     1741 agacccggac gacgacctac acgttcgagg agccggggaa gctgctggtc ggctgccacc
     1801 tgccgacgca ctgggactac ggcatgcgag gcgagataac cgtcacgggc tgagccagag
     1861 cgccccggcg atcgtgtagg cgacgacggc gctgtagccg accaccggga tccagagagc
     1921 ccgcggccgc agcttccaca gcacgaacga cagagccgcg acgaacacga ccttgcccgg
     1981 cagcgtcatc cagccgacga gagggttgcc ctccacggcg aggcccgcgc tcgtgacgag
     2041 gtaggtgagc agcgcgtcga gcaggttgag ggcgttgagc gccacgagca ggacgaagtc
     2101 ggtattgcga cgcggatgtg tctctgcggg ggccggccgg ggcgggtcgc ggaggtcgat
     2161 gacgaggtcc cgctcggcga cgcgcgtagg caccgggcca tcgtacgtgc ttgctcgcct
     2221 gtgatcgcag gagcggcggc acgtgcggcg aaatgtccga ccatgcgcag acctctcgcc
     2281 gcgttcggcg cggccctcgt cgtgttgtcc ctcgcccctg cctcggctcc ggcccggccg
     2341 gccgtcgccg ccggcgacga cgtgacggtg atcgccgtca tcgacgacgg catcacgccg
     2401 taccattggg actacctcgc gtcgaagatg ctggcctcgc cggacgtccc gctcgaccgg
     2461 ccgccgcacg agtggctccg cggcttcccg tcaccgggtg cgttcgccga ctacgagccg
     2521 ctcgatctga cgctcgagga gaagaacgcg aacgaaagcc ccgaagcgct gcactcgaag
     2581 gacctcgagg agtgggactc ggtcgagcag tcgacccccg cgaaggtcaa ctactactgg
     2641 atccccggca cgaagatcat cggcgccgtc gacttcggca ctcagaagat ctacgggccg
     2701 acgaacacgc acggcaccgg cgcgtcgagc gtcgccgtcg gcaacatcca cggctcgtgc
     2761 cccgagtgcc tgctcgtgtt cctcgacacc ggcaacttca accaggcgca gaccgaggcg
     2821 gcgctggagt gggccgagag ccagccgtgg atcgacgcgg tctcgaactc ctacggtcac
     2881 agcaccgtct tcaccgacaa cgtgtacacg ggctccgaca ccgaggcgca gaagcgcgcg
     2941 acggtgcgag gccagacggt cttcttcgcc gcccacaacg gtcacgacag gtcgttcttc
     3001 gtccccaaca acacgctgtt gtcgtcgcag ccgggcccgg actgggtcgt aaccgtcggc
     3061 gcggtcgacc cggcgagcag gggctcgttc accgggcacg gcaagcccgc cgacgtctcc
     3121 ggcgtcgggt tgtcgtatcc gtcggcctac ggttcgtcga ccgtgggggg cgagggcttc
     3181 ttctccggga cgtcgaacgc gacgcccgtc gtcaccggga tatacgggcg cgcgctgtac
     3241 gaggcgcggc tcgcgctccg cggtccgagc cgcgtgcagt cgaagggtgt ggtcgcccgc
     3301 ggctcggcgg tgcgctgcgg aaacgcgcgg cccgagtgcg agctcggcga cggcgtgctg
     3361 accgcggacg agctacggac ccggctgttc gaaggagcgg tgcacaccga ggccgggatg
     3421 acgtcgggag ccggcgtcgt cgcggggccc ccgctcggcg aggacgagtt cttgaacgag
     3481 ggccacggga cgtacctcgg cctcctcgac ggcgagaaga cgtggctcga ggagatcgca
     3541 cgtgtcgtcg gcccgatgct gggccgtgcg aaggtgctgg accgccccga aggcgagcgg
     3601 gagtggatgg tcgtcgactc gttctgccgc cagcacctat ggggctcgtg gaccgggggc
     3661 tattacgtag agggcaagac ggacctgccg ggtacgtcgc cggactggcc gctgcggagc
     3721 ctgatagaga ccgcctgccc gaacgtcccg cccttctaga tagcgtgtcg ccgtgaaggc
     3781 gatacgcgtg catcagctcg gcgggcccga ggtgctcgtc ctcgacgact tgtccgatcc
     3841 cgtggctcac gatggcgagg tcgtcgtgcg gctcgaggct gtcggcgtca acttcgtcga
     3901 cgtctaccac cgcaccgggc tctacccccg tgaccttccg gtcgtccccg gctccgaggg
     3961 tgcgggagtc gtcgtcgagg ccgcgccgga gacggtcttc tccgcaggcg atcgcgtcgc
     4021 ttccgctcgg ctggcgggcg cgtacgcgga ggccgcgacc gtgccggccg aggccctcgt
     4081 gccgatcccg gacggcgtcc cgaccgaact cgccgcggcc gtgatggtgc aggggatcac
     4141 cgcgcactat ctgttgaggt cgacgtgcga gctggacgaa ggcgactggt gcgtcgtgca
     4201 cgcggccgcg ggaggcgtcg gccagttgct gacgcagatg gcgcgcctgc aaggagtccg
     4261 ggtgctgggc acggtgtcga ccgaggcgaa ggccgaggtt gcgcgctctg cgggggccga
     4321 ggtcgtcgtg gtgcacgggg gccggagctt cgccgaggcc gctcgcgagg tcacggacgg
     4381 cgccggtgtg cgggcggtct tcgacgggat cggcaaggac acgttcgacg agagcctcga
     4441 ctgccttgcg ccgcgcggcc acatggtgct ctacggccaa gcgagcggga aggtcccccc
     4501 gatggacccg ctgcgattgg cgaagggatc actgttcctg acgcgcccgg gcatagccga
     4561 ctacacggcg actcgcgagg aactgctatg gagggccgaa gacgtgctga gcgccgtggg
     4621 ggccggcacg ctgagcgtgc acgtgcacga ccgctacccg ctggccgaag cgagccgcgc
     4681 gcacgccgac ctgcaatcga gaaagacgac gggcaagctc ctgctggtgc cctaaggaag
     4741 ctggctagac gccgaaccca agttgctgtg cccccgcgtg cgtattggct ggaggatgtt
     4801 ccccgaccag agcctcgaca tcggatatag cgcgcgctcc tgcgtggatc agtgcggcgt
     4861 tccctctgcc ttccccggcg cctgcccaga ccaggacgtc ttcaggggcc ctcttcaacc
     4921 attcggtcgc gccggcccac gtaccgcccg tctcgaggtc cgactgcacc acgagggtct
     4981 ttcgcgccaa ggcgtagacg agcttgttgc gcgccatggc gttcgcaacc gtgaagccca
     5041 tgctcggctt gtaaggagtg aggagacaca cttgctcgtc gtggatagcg cggcgcgtgt
     5101 cggggtcgcg cagccgccgt tcgagcgagt ctgcgagaac gccgacgact gcgccgcccg
     5161 cgtcgaacgc ggcgttcatc gctacctggt cgattccttt ggctgctccc gaaaagaccg
     5221 ttaggccctg cgaggcgagg tagcgggcag cgtcgccggc gactcgggct gcctccgggc
     5281 ctacgtcccg agctccgacg atcccgatgc cacttgtcgc catgagatcg gtcgaaccgg
     5341 ccacg
//
LOCUS       DASQDO010000143         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_75687, whole genome shotgun sequence.
ACCESSION   DASQDO010000143 DASQDO010000000
VERSION     DASQDO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75687"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEV43_04305"
     CDS             <1..800
                     /locus_tag="VEV43_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP22774.1"
                     /translation="NLINQKVAVAMLSGARYLVDTVLNGAAAVKAVADTRYDVILMDC
                     QMPELNGYEATAAIRAQDGTDRHTPIIAMTAGARDEDRARCFAEGMDGYLTKPVDKAA
                     LLALVARSLPEATRTDVSPDAGVTATAAITLDPSTAGAPSADEAFDPPEAPPVLDAEV
                     VGRLVSLGDGTDEDFMGQLTLLFTAEADIRVAELRGALAAGDEDLLARVAHNLRGASA
                     NLGALGLARLCGVLEATGLAEGESTVESIALEVERVRSALEARIPAS"
     gene            797..1105
                     /locus_tag="VEV43_04310"
     CDS             797..1105
                     /locus_tag="VEV43_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP22775.1"
                     /translation="MRILVADDEPSSRLIARVAVQTLGHECETVSDGAEAWEAFRSRP
                     HDVVISDWLMPGLTGLELCRNIRAHSAPGYPYFIMVTGQGARDQVVEGMTAGADDYLV
                     "
     gene            1217..1762
                     /locus_tag="VEV43_04315"
     CDS             1217..1762
                     /locus_tag="VEV43_04315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYP22776.1"
                     /translation="MTEVSLRDALTGLGNRMALDEDLDQLQARVTRYGHRYCVALLDV
                     DHFKAYNDSYGHQAGDQALRTVAAQISAQARGGDAVYRYGGEEFLCIFPEQTLETAAI
                     AAERMRSSLEQLAVPHGDSDFGVLTMSGGLAILDSSGSKSVGEVLKDADEALYLAKRL
                     GRNRIERASRVASEHSTFARM"
     gene            1766..3406
                     /locus_tag="VEV43_04320"
     CDS             1766..3406
                     /locus_tag="VEV43_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYP22777.1"
                     /translation="MALVMPPSANNPASPDDNAFSSVETAPRSNPRELAARHGLEFVD
                     LAREQVEPAAADAIPLALLERLGAVPYRLAGDHLSVAIPDPADLGVIDQLRLSTRFTV
                     DVAVASRQEIQNALKRIGRGRQFTARASAMDVVSLEVDGPPDVEADETFDLEAAERGG
                     DAPPIQLVNSIILQAAEDGASDIHFLPRSDGIAVRVRIDGVVHEVDRIPRRHAAAVVS
                     RLKVLAKLDIAEHRRPQDGRISLRATRSGQLLDARLAVLPTVDGEGVVMRLLDKSRRA
                     PTLTEIGLSNTMQMAVEQAIHRTSGSLLCTGPTGSGKSTTVYAALADIIRPEISVITI
                     EDPVEYRLHDIYQMQVDPRWGLSFATALRSILRGDPDVIMVGEIRDLETAKIAVEAAM
                     TGHFVLSTLHASDAPGALTRLAEMGVEPYFTSAAISGVVSQRLIRRLCLECREPYEPR
                     AEEVASLYLPPEFDGELTLYRKRGCGSCFKGYSGRIGVYQYMDVGNEIKELVSRGATS
                     AELEQAAVDGGMRSLWADGSEKVAGGLTTAEELHRMLR"
     gene            complement(3413..>3993)
                     /locus_tag="VEV43_04325"
     CDS             complement(3413..>3993)
                     /locus_tag="VEV43_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP22778.1"
                     /translation="APCRGHATSPRRRSKSVGEGPVRGPATAGDRVGTAESASGSAFP
                     TRGFTCAAGVRRILAAELTAGDYLQDDQTLILLAEDSPSVQAMVSFRLERSGYDVLVT
                     DNGDDALRLARERLPALAVLDVEMPGLSGLEVTRRLRAEDQTRDMPVILLTSLDGELA
                     VAEGYEAGANDYVTKPFSPQELQTRVKGLLGT"
BASE COUNT          637 a         1423 c         1340 g          593 t
ORIGIN      
        1 acaacctgat caatcagaag gtcgcggtgg ccatgctgtc gggcgcgcgc tacctcgtcg
       61 acaccgtcct gaacggggcg gcagcggtga aggccgttgc cgatacgcgc tacgacgtga
      121 tcttgatgga ctgccagatg cccgagctga acggctacga ggcgacggcg gcgatccggg
      181 cccaagacgg cacggaccgc cacacaccga tcatcgccat gaccgcgggg gcgcgagacg
      241 aggaccgcgc gcgctgcttc gcagagggca tggacggcta cctcacgaag ccggtcgaca
      301 aggctgccct tctcgcgctc gtcgcccggt ccctccccga ggcgacgcgg acggacgtgt
      361 cgcctgatgc cggggtcacc gcgacggcgg cgatcacgct cgacccgtcc accgccggcg
      421 cgccgtcggc cgatgaggcg ttcgaccccc ccgaggcccc acccgtcctc gatgcggagg
      481 tggtcgggcg cctggtcagc ctgggcgacg gaaccgacga ggatttcatg ggccagctga
      541 ccctcctgtt caccgcagag gccgacattc gcgtcgccga gctgcgaggc gcgctcgccg
      601 ccggcgacga ggacctcctg gctcgagttg ctcacaacct ccgcggcgcc agcgccaacc
      661 tcggtgctct ggggctagcg cgcctctgcg gcgtcctgga ggcgaccggc ctggcggaag
      721 gcgaatcgac tgtggagtca atcgcactcg aggtcgaacg cgtccgctcg gcgctcgaag
      781 cccggatccc cgcatcgtga ggatcctggt cgccgacgac gagccatcct cgcggctcat
      841 cgcgcgagtg gccgtgcaga cgctcggaca cgaatgcgag accgtgagcg acggcgcgga
      901 ggcgtgggag gcctttcggt ctcgtcccca cgacgtggtc atcagcgact ggctcatgcc
      961 cggcttgacc gggctcgagc tttgccggaa catccgcgcg cactccgcgc ccggctaccc
     1021 ctacttcatc atggtcacgg gccagggcgc ccgcgaccag gtcgtcgaag gcatgacggc
     1081 cggggccgac gactacctcg tctagtcact ggacgccgac gtcctccagg cccgcctgat
     1141 cggcgccacg agggtcacgt ctctccaccg cgagctcgac gagcggcgcg acgagctgga
     1201 ggcgctgaac cgatcgctga cggaggtctc cctcagggac gccctgaccg gtctcggaaa
     1261 ccgcatggcc ctcgacgagg atctcgacca gctgcaggcg cgagtgactc gctacgggca
     1321 tcggtactgc gtggcgctcc tcgacgtcga tcacttcaag gcgtacaacg acagctacgg
     1381 ccaccaggcg ggcgaccagg ccctccgcac cgttgcggcc cagatcagcg cgcaggcacg
     1441 tggcggcgac gcggtctacc gctacggcgg cgaggagttc ctctgcatct ttccggagca
     1501 gactctcgag accgcggcga tcgccgccga gcgcatgcgc tcgagcctgg agcagctggc
     1561 cgttccacac ggtgacagcg acttcggcgt cctcaccatg agcggcggcc tggccatcct
     1621 cgactcgagc ggcagcaagt ccgtcggcga ggtcctcaag gacgcagacg aggctctcta
     1681 cctcgccaag cgtctcggcc ggaaccgcat cgagcgggcc tcgcgcgtcg cctccgagca
     1741 ttcaacgttt gcccgcatgt agccgatggc tcttgtgatg ccgccttccg cgaacaaccc
     1801 cgcttctccc gacgacaacg ccttctcctc cgtcgagacg gcgccgcgct cgaacccgcg
     1861 cgagcttgcc gcacgccatg ggctcgagtt cgtcgacctc gcccgggagc aggtcgagcc
     1921 ggcggcggcc gacgcaatcc cgctggccct gctcgaacga ctcggcgccg tgccctaccg
     1981 cttggcgggc gaccatcttt cggttgcgat cccggacccc gcggatctcg gcgtgatcga
     2041 ccagctccgc ctctcgacgc ggttcacggt cgacgtcgcc gtcgcgtccc gtcaggaaat
     2101 ccagaacgcg ctcaagcgca tcgggcgagg acgccagttc accgcgagag cctccgcgat
     2161 ggacgtcgtt tcgctcgagg tggacgggcc gcccgacgtc gaggccgacg agaccttcga
     2221 cctggaagcc gccgagcgcg gcggagacgc gccgccgatc cagctcgtga actcgatcat
     2281 cctccaggcc gcagaggacg gggccagcga catccacttc cttccgcgct cggacggtat
     2341 cgccgtgcgc gtgcgcatcg acggtgtcgt tcacgaggtc gatcggatcc cgcgacgcca
     2401 tgcggcggcg gtcgtctcga ggctcaaggt cctcgcgaag ctcgacatcg ccgaacatcg
     2461 ccggccgcag gacggacgca tctccctccg cgcgacccga agcgggcagc tcctcgacgc
     2521 gcggcttgcg gtcctgccga cggtcgatgg ggagggcgtg gtcatgcggc tcctggacaa
     2581 gtcccggcga gcgccgacgc tcacggagat cgggctctcg aacaccatgc agatggcggt
     2641 cgagcaggcg atccaccgga cgagcggatc gctgctctgc accgggccca ccggctcggg
     2701 caagtcgacc acggtctacg ccgcactcgc cgacatcatc cgccccgaga tcagcgtgat
     2761 caccatcgag gatcccgtcg agtaccgact tcacgacatc taccagatgc aggtcgaccc
     2821 tcggtggggc ctttcgttcg cgacggcgct gcggtcgatc ctccgcggcg atcccgacgt
     2881 gatcatggtc ggcgagatcc gcgacctcga aacggcgaag atcgcggtgg aggcggccat
     2941 gaccggtcac ttcgtgctct cgacgctgca cgcgagcgac gcgccggggg cgctcacgcg
     3001 gctggcggaa atgggcgtcg aaccgtattt cacgagtgcc gcgatcagcg gcgtcgtcag
     3061 ccaacgactc atccggcggc tttgcctgga gtgccgggag ccctacgagc cgagggcgga
     3121 ggaggtcgca tctctgtacc tgccgcctga gttcgacggc gagctcacgc tctatcgcaa
     3181 gcgcgggtgc gggagctgct tcaagggcta cagcggtcgg atcggcgtgt accagtacat
     3241 ggacgtcgga aacgaaatca aggagctcgt ctcacgcggt gccacgagcg cggagctcga
     3301 gcaggcggcc gtcgatggcg gcatgcgaag cctctgggcc gacggctccg agaaggtcgc
     3361 aggcggtctg acgacggccg aagagctcca ccgcatgctc cgctagccgg gctcacgtcc
     3421 cgagcagccc tttcacgcgc gtctggagct cctgcgggct gaacggtttc gtcacgtagt
     3481 cgttcgctcc cgcctcgtac ccttccgcga ccgccagctc cccgtccagg gaggtgagga
     3541 ggatcaccgg catgtcccgg gtctgatcct ccgctcgcaa cctacgggtc acctccaggc
     3601 cgctgagccc gggcatctcg acgtcgagca cggcgagtgc cggcagccgc tcgcgcgcga
     3661 ggcgaagcgc atcgtctcca ttgtccgtga cgaggacgtc gtagccggag cgctcgagcc
     3721 ggaacgacac catcgcctga accgacggcg agtcctcagc caggaggatg agcgtttggt
     3781 cgtcctggag gtagtcgccg gcggtcaact cggcggcaag gatacgacgc acgcccgccg
     3841 cgcaggtgaa tccgcgggtc ggaaacgcgc ttccgctcgc cgactccgca gtaccaaccc
     3901 gatcgccggc ggtcgcggga ccacggacgg gtccttcgcc gacgctcttg gaacggcgcc
     3961 gtggcgatgt ggcgtggccc cggcacgggg ctc
//
LOCUS       DASQDO010000144         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_932244, whole genome shotgun sequence.
ACCESSION   DASQDO010000144 DASQDO010000000
VERSION     DASQDO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932244"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VEV43_04330"
     CDS             complement(<1..1070)
                     /locus_tag="VEV43_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22779.1"
                     /translation="MIPKRFVAALSVVALGAGMLSIGPTASASHATVSIQVGAQLGGA
                     NLPAESMRFFGPETITVHQGDRITFDFRGFHTATLLPAGAGADDWLADNATPTGQYGF
                     AKSDPDDGANEFKDNFPAIVSPTDATCGAGGQSPCSYTGDDVLNSGAPIEAGQTFTVT
                     VSSEPGSTFWVVCLIHHHMRKRIRVVADPASATPQSAIDEVRREQAARDLDWAKATHT
                     KFSTRQTSHENAAGRRVWDAWAGVDSRFVSLYAFYPKRLKVDKGDIVRWRFDQLVHED
                     HTVSMPDPGIFRHLRFDEEMCDPDGDQGTQPDTVPERDQQNGEAICPEGSTLEVDVSP
                     QFWGGTGNGVLSGMNDAEHSGIR"
     gene            complement(1179..>1758)
                     /locus_tag="VEV43_04335"
     CDS             complement(1179..>1758)
                     /locus_tag="VEV43_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22780.1"
                     /translation="ARATFLGALSDLTRGDPVLVVDIGGGSTELIVGRDEPERLVSLD
                     VGCVRMLEKHLESDPPAPGELEALRAEVSGALDEVKGDLAVPAGARMIGVAGTVTQLA
                     SLKSGALVYDPDVTHHSVLSHGDGRRLARRLETLTYEQRRRVRGLEPGRADVIVAGAE
                     ILLCAMETFDAAEVLVSEKDILDGLVIQLLAS"
BASE COUNT          263 a          629 c          573 g          293 t
ORIGIN      
        1 cggatccccg agtgctcggc gtcgttcata cccgacagga caccgttgcc ggtcccgccc
       61 cagaattgag gggacacgtc gacctcgagc gtgctgccct cgggacagat ggcctccccg
      121 ttctgctgat ccctctccgg caccgtgtcc ggctgggtcc cctggtcgcc gtccggatcg
      181 cacatctcct cgtcgaaccg gagatggcgg aagatcccgg ggtcgggcat cgacaccgtg
      241 tggtcctcgt ggacgagctg gtcgaacctc catctgacga tgtcgccctt gtcgaccttc
      301 aggcgcttcg gatagaacgc gtacagcgac acgaaacggc tgtcgacgcc cgcccacgcg
      361 tcccacaccc gccgccccgc ggcgttctca tgagacgtct gccgggtgct gaacttcgtg
      421 tgcgtagcct tcgcccagtc gaggtcgcgc gccgcctgct cgcgacgtac ctcgtcgatg
      481 gcgctctgag gcgtcgccga cgcggggtcg gccaccactc ggatccgctt gcgcatgtgg
      541 tggtggatga gacagacgac ccagaaggtg ctcccgggct cggagctcac cgtcacggtg
      601 aacgtctgac ccgcctcgat cggcgcaccg gaattcagga cgtcgtcacc cgtgtagctg
      661 cacgggcttt gtccacccgc gccgcacgtc gcatccgtcg gcgagacgat cgcggggaag
      721 ttgtccttga actcgttcgc cccgtcgtcg gggtccgact tcgcgaaccc atactgcccc
      781 gtcggggtcg cgttgtcggc gagccagtcg tccgcaccgg cgccggcggg gaggagcgtc
      841 gccgtgtgga agccgcggaa gtcgaacgtg atcctgtcgc cctggtggac cgtgatcgtc
      901 tcgggcccga agaaccgcat ggactcggcg ggcaggttgg cacccccgag ttgggccccc
      961 acctggatcg agacggtcgc gtgacttgcc gacgcagtcg ggccgatgct cagcatccct
     1021 gcaccgagcg caacgaccga cagcgctgct acgaatctct tcgggatcaa tcccgccacc
     1081 tttcctctag gggtcctcga gcccgcgaag cgaggcaatc ccgctagacg tactagaagg
     1141 cggcgcccgt gtcgaggtat gtgcggtgga ttcgtatgtc agcttgccaa cagctgaatg
     1201 acgagtccgt ccaggatgtc cttttccgag acaaggacct cggctgcgtc gaaggtctcc
     1261 atggcgcaca gcaggatctc ggcccccgcc acgatcacgt ccgcgcgccc cggctccagg
     1321 cccctgaccc tcctccgctg ctcgtaggtg agcgtctcga gcctgcgggc gaggcgcctc
     1381 ccgtctccgt gcgagaggac cgaatggtgc gtgacgtccg ggtcgtacac gagcgccccg
     1441 gacttcagcg aagccagctg cgtcacggtt cccgcgacgc cgatcatgcg ggccccggcc
     1501 gggaccgcga ggtcgccctt cacctcgtcc agcgcgcccg agacctcggc ccgcaatgcc
     1561 tcgagctccc cgggggccgg agggtcggac tcgaggtgct tctcgagcat ccgcacgcat
     1621 ccgacgtcga gcgagacgag ccgctcgggc tcgtcgcggc ccacgatcag ctcggtcgat
     1681 cccccgccga tgtcgacgac gagcacgggg tcgccgcggg tcaggtccga gagcgcgccg
     1741 aggaacgtcg cccgtgcc
//
LOCUS       DASQDO010000145         5941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_137457, whole genome shotgun sequence.
ACCESSION   DASQDO010000145 DASQDO010000000
VERSION     DASQDO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137457"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VEV43_04340"
     CDS             complement(<1..1058)
                     /locus_tag="VEV43_04340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP22781.1"
                     /translation="MLVIAPFSGATGLGRIARGIARSLDDADVHVLSLDAGDVDPASL
                     PPRPLTIHPNTTPADPFARDDAASLTRELNPDVVLIAHAVWPLGRLARAARSEQPDVP
                     VVGYLPLDGRINEPAAFSHLGDLHTLVACTRFGATELERACVRAHVPVIPHAIDDRFK
                     PLVPPPGPMDSRSAARAELWKGRSDLANAFIFLNANKNDGRKRIDITLQAFAELLRRT
                     GAEDAWLSLHMGTGDSGTELVDLIYRLGISERVLLRGVRHPSDTDGRLNLHYNACEVG
                     VNTSMGEGWGLVAFEHAATGAAQVISRNPVAEELWDGGARLVPTRPTPPVEALELMEA
                     DPFDVAAEMIALYSDQGL"
     gene            complement(1148..1543)
                     /locus_tag="VEV43_04345"
     CDS             complement(1148..1543)
                     /locus_tag="VEV43_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22782.1"
                     /translation="MDTIVYKFVTHDATEGERRRGKMVVNFINFYNVYLQNTTNCHLY
                     LIRCGVNLRPDETIRIQCDCEGITDVAGGVMRRYHWTTGRAVNVPSHAIISECNATHV
                     RMDIHTPPQPEERVYARFVGNLELVTPDD"
     gene            complement(1694..2863)
                     /locus_tag="VEV43_04350"
     CDS             complement(1694..2863)
                     /locus_tag="VEV43_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22783.1"
                     /translation="MRPRIALLAGMSLLAGLVNAPAGAGPTTGGLASDNVEHVAHVPI
                     AQNGVGGRLIGKHFYMNDQNKIMIFDVSTPEDPQLTGFVTMPQEWQFSREDIDGNGDI
                     LVVPNTANGVADGSPPSGGGATAAANAVYVIDVEDKTNPQIISKINGPAQHTYSCVLD
                     CKWAWGSEGAIIDLRDPANPVLMEEKWGDGMPAQGGHDVEEIAPGLLVTATQPVMYLD
                     ARKDPLHPKLLAKGGNEDNRYMHGARWPQRGRDKFLLMAGETTFNLQCDETSGAFMTW
                     DARKWRKTKTFTMIDEYRVENGTWVDGNPAVQRNCTSHWHEEHPDFRNGGLVAVAFYD
                     HGTRFLEVSKTGKISDAGWFMPYGGQTSAVYWITDEIVYSVDYNRGLDVLRFNAD"
     gene            complement(2866..3336)
                     /locus_tag="VEV43_04355"
     CDS             complement(2866..3336)
                     /locus_tag="VEV43_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22784.1"
                     /translation="MRRPLVAGLAACLLLGAAVAPATAKKKKPRKPPVTFEATGALAL
                     GHPGDVAAGANVTRTAFLETCAIPPSQGTDGYVIELPPAVTAVPTNVTITGVSAGDLH
                     DLDVHFFGDGCSSLGGLATEQVGEFGLMPAGTKYVLVTAFMGVQTDFVFKAEGV"
     gene            complement(3401..>5941)
                     /locus_tag="VEV43_04360"
     CDS             complement(3401..>5941)
                     /locus_tag="VEV43_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22785.1"
                     /translation="PHAHSTTQRDSYGVHDRLSYRAIVVQDGQGDQAAFVKSDSYLAQ
                     DHLSRRVGQILESEGSSVSYEEIFHMASHNHSSPYYQSPSAGVWIFQDAFDLRAFEYH
                     ARQMAAAILEAEDNLVPARMGATTITHRIFKGMIARKDVGDDNTPVGYPDDFGDFGLA
                     VVRFDDMSNPSAPKPLATLINWGQHPEGLDDHDLITGDFVAPLERFVERATGAPLVFG
                     QGDVGSAEAGPGRPDLVPAGVPKKWSHAGHAQAERGAFFLSQDVVKGWNEIGAGRALV
                     AYSTSFDVEAGNAWVPGPLSHPYPSFGNCRIGAAAEGQPRAPGVPTCNGAGTHDKASM
                     MWENLRAHGIPVPEHVSAPAFSGVEENLRLHLQAFKLGEVIFASCACEAQVDLILNFE
                     TRADEAQGNIWHGYDWTERLGCTQGATGAWTCKNPRNLNQTITFSDARYRRMKAQIHN
                     DAKGWDSPQNAATAESESADPSKIFGNFTHEELPSSLGYKLPIGVGHAGDYNGYTVSY
                     REYMAYDHYRKALTAYGPHTADYMVTRLVKLAGELNGGPELPPEPHDVTAQADEARQV
                     AASTALGAASSQAYDAWLRALPDDAGPAEATGQPEDVTKFDAAYFSWRGGSNAADNPV
                     VRVERLVDGSWQPFADQSGEVQTMVEFPDGMDGLLNTYQGNEEWIWTAGFEAFDAFPV
                     EIGSTPPGEYRFVVDGHIRQGGESLPYHLESASFQVKPWDGVEVTDLRVDPGAVSFAA
                     AGDYPETYTGSPFPYVRVERKNDEVGVPFCTTCSFRPWASSGAIVEAAVTVVRSTGAV
                     EVVPAQLIDGRWVAPVELAPGDSAYVEAGAVVDEHGERNAARSATVTG"
BASE COUNT          884 a         1980 c         2052 g         1025 t
ORIGIN      
        1 aagaggcctt ggtcggagta caaggcgatc atctccgcgg cgacgtcgaa cggatcggcc
       61 tccatcaact cgagagcctc taccggaggc gtcgggcgag tagggacgag gcgggcgcct
      121 ccgtcccaga gctcctcggc gacggggttc cgagaaatca cttgcgcggc ccccgtggcc
      181 gcgtgctcga acgcgacaag accccagcct tcgcccatgg acgtattcac cccgacctca
      241 caggcgttgt agtgaaggtt gagccgaccg tcggtatcgg acgggtggcg taccccgcga
      301 agcaggactc gctcggagat cccgaggcgg taaatcaagt cgacgagctc cgttccgctg
      361 tcgccggttc ccatgtgcag actgagccag gcgtcctcgg caccagtacg gcggaggagc
      421 tccgcgaatg cctgaagggt tatgtctatc cgtttacgac cgtcgttctt gttcgcgttc
      481 aggaagatga acgcattcgc taggtcggac ctgcccttcc acagctcggc tctcgccgcc
      541 gagcggctat ccatgggacc cgggggaggc accaaaggct tgaaccggtc gtcgatggcg
      601 tgcgggataa cggggacgtg ggcccgcaca catgcacgct cgagctccgt tgcgccgaaa
      661 cgtgtgcagg cgacgagcgt gtgaaggtcg ccgagatgcg aaaaggcggc gggctcattg
      721 atcctgccgt ccaacgggag atacccgacg accgggacgt cgggttgctc ggaccgcgcc
      781 gcgcgagcaa gtcggccgag cggccacacc gcgtgggcga tcaggacgac gtccgggttc
      841 agctcgcgcg tcaggctggc ggcgtcatcg cgcgcgaagg gatcagcggg cgtcgtgttc
      901 gggtggatag taagaggccg cggcggcaac gacgccggat cgacgtctcc agcgtccaag
      961 gacaggacgt ggacgtccgc gtcgtccagc gaacgtgcaa tcccgcgtgc gatccttcct
     1021 aggccggttg ctccggagaa cggggcgatg acgagcacac ggggcagcga cgaccccagc
     1081 cggtgcctca ctagctcggc atccgccgac tacccgcccg ccacggtact gggccaggtc
     1141 ccgtcgctca gtcgtcgggt gtgacgagct cgaggtttcc gacgaatcgg gcgtataccc
     1201 tttcttcggg ctgtggcggc gtgtggatgt ccatcctcac gtgcgttgca ttgcactcac
     1261 tgatgatggc atgcgaaggt acatttaccg cccttcccgt cgtccagtgg taccgcctca
     1321 tgactccgcc ggccacgtcc gtaatgcctt cgcagtcgca ttgaatgcgg atcgtctcgt
     1381 cgggacgcaa gttgacgccg catctaatga gatagagatg acagttcgtg gtgttttgta
     1441 ggtagacgtt gtagaagttg atgaagttga cgaccatctt gcctcgcctt ctctcaccct
     1501 ctgtggcgtc gtgcgtcacg aatttataca cgatggtgtc cacgagacaa gtaggaagtc
     1561 aaaatcgagc gactgaggca cccggtgccg gcctcagaca ggagctcgcg accaagcaag
     1621 aacagcacgg ctcgaagggc tcgccggctt catgggacga tttcaaggct tctcacggcc
     1681 gcgtgagggt cggtcagtcg gcgttgaaac gcaggacgtc caggccccgg ttgtagtcca
     1741 ccgagtacac gatctcgtcc gtaatccagt acaccgcgct cgtctggccc ccgtagggca
     1801 tgaaccagcc cgcgtccgag atcttgccgg tcttgctcac ctcgaggaac ctcgtgccgt
     1861 ggtcgtagaa cgccaccgcc accaggccgc cgttgcggaa gtccgggtgc tcttcgtgcc
     1921 agtgcgacgt gcagttgcgc tggacggccg ggttcccgtc gacccaggtc ccgttctcga
     1981 cccggtactc gtcgatcatc gtgaacgtct tcgtcttgcg ccacttgcgc gcgtcccacg
     2041 tcatgaacgc gccggacgtc tcgtcgcact gcaggttgaa cgtcgtctcg cccgccatca
     2101 gcaggaactt gtcacggccc cgctgcggcc agcgcgctcc gtgcatgtag cggttgtcct
     2161 cgttgcctcc cttggcgagc agcttcgggt gcagcgggtc cttgcgcgcg tcgaggtaca
     2221 tgaccggctg cgtcgccgtg acgaggagcc cgggcgcgat ctcctcgacg tcgtgcccgc
     2281 cctgcgccgg catgccgtcg ccccacttct cctccatgag cacggggttc gccgggtcgc
     2341 gcaggtcgat gatcgccccc tcggaccccc acgcccactt gcagtcgagc acgcagctgt
     2401 aggtgtgctg ggccgggccg ttgatcttcg agatgatctg cgggttcgtc ttgtcctcga
     2461 cgtcgatcac gtacacggcg ttcgcggcgg cggtcgctcc cccaccgctc ggcggagacc
     2521 cgtcggcgac gccgttcgcg gtgttcggca ccacgaggat gtcgccgttg ccgtcgatgt
     2581 cctcgcggct gaactgccac tcctgcggca tcgtgacgaa gcccgtcagc tgcgggtcct
     2641 cgggggtcga gacgtcgaag atcatgatct tgttctggtc gttcatgtag aagtgcttgc
     2701 cgatgagccg gcccccgacg ccgttctgcg cgatcggcac gtgggcgacg tgctcgacgt
     2761 tgtcgctggc gaggccgccg gtcgtcgggc cggcaccggc cggggcgttc acgagcccgg
     2821 ccaggagcga catccccgcc aggagggcga tccgaggacg catggctaga ccccctcggc
     2881 cttgaagacg aagtcggtct gcacccccat gaacgcggtg acgagcacgt acttcgtccc
     2941 ggccggcatg aggccgaact cgccgacctg ctcggtggcg aggcccccga gcgacgagca
     3001 gccgtcaccg aagaagtgga cgtcgaggtc gtgcaggtcg ccggcggaca cgccggtgat
     3061 cgtcacgttc gtcgggaccg cggtgaccgc gggcgggagc tcgatgacgt agccgtccgt
     3121 gccctgcgag ggcgggatcg cgcacgtctc gaggaacgcg gtgcgggtga cgtttgcacc
     3181 ggcggcgacg tcaccggggt ggccgagggc gagcgcgccg gtcgcctcga aggtcaccgg
     3241 aggcttgcgc ggcttcttct tcttggccgt cgcaggcgcg accgccgcgc ccaacagaag
     3301 acatgctgcg agtcctgcta cgagcggtcg gcgcatctgg atacctccgt tgtctgaagt
     3361 ggtaccggtt tattaggcgg cggggggccg gggacctgcc tcagcccgtg acagtggccg
     3421 aacgggccgc gttgcgttcc ccgtgctcgt cgacgaccgc acccgcctcg acgtaggccg
     3481 agtcacccgg ggccagctcg accggagcga cccaccggcc gtcgatcagc tgggccggaa
     3541 cgacctcgac cgccccggtc gaccgcacga ccgtgactgc ggcctcgacg atcgcgcccg
     3601 acgacgccca cggccggaag ctgcaggtcg tgcagaacgg gacgccgacc tcgtcgttct
     3661 tcctctcgac cctcacgtac gggaagggcg agccggtgta ggtctcgggg tagtcgccgg
     3721 cggccgcgaa cgagaccgcc cccgggtcga cccgcaggtc cgtcacctcg acaccgtccc
     3781 acggcttcac ctggaacgac gccgactcga ggtggtacgg cagcgactcg ccgccctgcc
     3841 ggatgtgccc gtcgacgacg aagcggtact cgcccggcgg ggtgctgccg atctcgacgg
     3901 ggaacgcgtc gaacgcctcg aagcccgcgg tccagatcca ctcctcgttg ccctggtagg
     3961 tgttcagcag cccgtccatg ccgtcgggga actcgaccat cgtctgaacc tcgccgctct
     4021 ggtcggcgaa cggctgccac gagccgtcga cgagccgctc gactcgcacg acgggattgt
     4081 cggccgcgtt cgagccgccg cgccacgaga agtacgcggc atcgaacttc gtcacgtcct
     4141 ccggctggcc ggtcgcctcg gcgggccccg cgtcgtccgg cagcgcacgc agccacgcgt
     4201 cgtacgcctg gctcgaggcg gcgccgagcg ccgtcgaggc cgcgacctgc ctggcctcgt
     4261 cggcctgggc cgtcacgtcg tgcggctcgg gcgggagctc gggccccccg ttcagctcgc
     4321 ccgcgagctt cacgagcctc gtcaccatgt agtccgcggt gtgggggccg tacgccgtca
     4381 gggccttgcg gtagtggtcg tacgccatgt actcgcggta gctgaccgtg tagccgttgt
     4441 agtcgcccgc gtggccgacg ccgatcggca gcttgtagcc gaggctcgac gggagctcct
     4501 cgtgcgtgaa gttgccgaag atcttcgacg ggtcggccga ctctgactcg gcggtcgcgg
     4561 cgttctgcgg cgagtcccag cccttcgcgt cgttgtggat ctgcgccttc atgcggcggt
     4621 agcgagcgtc ggagaacgtg atcgtctggt tgaggttcct ggggttcttg cacgtccacg
     4681 ctccggtcgc gccctgcgtg cagccgaggc gctcggtcca gtcgtagccg tgccagatgt
     4741 tgccctgggc ctcgtcggcc ctcgtctcga agttcaggat caggtccacc tgggcctcgc
     4801 aggcacacga ggcgaagatc acctctccga gcttgaaggc ctggaggtgc aggcggaggt
     4861 tctcctcgac gccggagaac gcaggtgcgc tcacgtgctc gggcaccggg atgccgtggg
     4921 ccctcaggtt ctcccacatc atgctggcct tgtcgtgggt gccggccccg ttgcaggtcg
     4981 gtacgcccgg ggcacgcggc tggccctcgg cggcggcgcc gatgcggcag ttgccgaagc
     5041 tcgggtacgg gtggctgagg gggccgggga cccacgcgtt gccggcctcg acgtcgaagc
     5101 tcgtgctgta ggcgacgagg gcccggcccg caccgatctc gttccagccc ttcaccacgt
     5161 cctgggagag gaagaaagca ccgcgctcgg cctgggcgtg gccggcgtgc gaccacttct
     5221 tcggaacccc ggcggggacc aggtcgggtc ggccgggccc ggcctcggcg ctgccgacgt
     5281 ctccctgacc gaagacgagc ggcgcgcccg tcgccctctc gacgaagcgc tcgagcggag
     5341 cgacgaagtc accggtgatg aggtcgtggt cgtcgaggcc ctcggggtgc tggccccagt
     5401 tgatcagcgt cgcgagcggc ttcggcgccg acgggttgct catgtcgtcg aagcggacca
     5461 ccgcgaggcc gaagtcgccg aagtcgtcgg ggtagccgac cggggtgttg tcgtcgccga
     5521 cgtccttgcg ggcgatcatg cccttgaaga tccggtgggt gatcgtcgtc gcgcccatgc
     5581 gagccgggac gaggttgtcc tcggcctcga ggatcgcggc cgccatctgg cgtgcgtggt
     5641 actcgaacgc cctcaggtcg aaggcgtcct ggaagatcca cacgccggcc gaggggctct
     5701 ggtagtaggg cgacgagtgg ttgtggctcg ccatgtggaa gatctcctcg taggagaccg
     5761 acgagccctc ggactcgagg atctgaccga cccgccgcga caggtggtcc tgcgcgaggt
     5821 agctgtcgga cttcacgaac gcggcctggt cgccctggcc gtcctggacg acgatcgccc
     5881 ggtagctgag gcggtcgtgg acgccgtagc tgtcgcgctg ggtggtcgag tgcgcgtgcg
     5941 g
//
LOCUS       DASQDO010000146         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1177267, whole genome shotgun sequence.
ACCESSION   DASQDO010000146 DASQDO010000000
VERSION     DASQDO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177267"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1207)
                     /locus_tag="VEV43_04365"
     CDS             complement(14..1207)
                     /locus_tag="VEV43_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HYP22786.1"
                     /translation="MPFRLTDVPAGSPYDAADMADVERKHWAHLYAEVVATEICCGCS
                     ACIVACPHHVLELSDWDPVQTDLSSPFDDCVHGQEGCSLCAMACLRLDPKIDVIETAV
                     HGKRRDQDQPEGTYRWKTLARATYAPILERGQDGGAVTALISWGLDTGELDGAAVSAP
                     SADVPWLDEPAIVQSPDELLDKAGSRYTYCATPLALKKAAEMKLKNIAVVGVSCESTA
                     VRNLAAEGIKRWTRRVGLVIGLMCCETFDYDAFMVGKVKHDLGIPLEDVKKVNVKGKV
                     ILTLNDGRDVDIPLKHARPFANEWCHHCPDFAAEHADLSCGGLGMEGWTMILVRSERG
                     EDYLRRAVDAGVVELRDAEEEPGALAVMDRLARKQRARVGPDDPHASAAFATVQALEA
                     ARAEA"
     gene            1206..1724
                     /locus_tag="VEV43_04370"
     CDS             1206..1724
                     /locus_tag="VEV43_04370"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HYP22787.1"
                     /translation="MKAFLGILIELVVAVTSPAPPDRVAPRAPAVNVYLLQEDAGGYR
                     RTAYQPLPRPARGARGPGERLQVALDELVRGTTRTERRSGYTSAFTRDTAGVIRRAEL
                     RGRRAVVDFRDFRDEQGQWGTAYGGSVFLMQVSRTIFQFERVRSALLRIDGSCRQFWE
                     FLQMRCEVLRRP"
     gene            1825..2469
                     /gene="cofC"
                     /locus_tag="VEV43_04375"
     CDS             1825..2469
                     /gene="cofC"
                     /locus_tag="VEV43_04375"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HYP22788.1"
                     /translation="MDAGLLPVKALGRAKSRLSPLFDDDQRRAVATALLEDALSLCAS
                     AGFLEWWVVSEDPEVLGRGSGAGLKTVTDPGGGLNAAVAAGIEEVVAAGAESVTVIPC
                     DVPLAYKGDLVDLVDTGATSDIVLVPSGGDGGTNALFLSPPGLIEPAFGPGSLQRHIA
                     LAEKERYRCAILSLPRLALDVDTPDDVDAFLASAGEFGGRSAEVLRKLREGQAP"
     gene            complement(2438..2875)
                     /locus_tag="VEV43_04380"
     CDS             complement(2438..2875)
                     /locus_tag="VEV43_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22789.1"
                     /translation="MKRTMTGGTHRGPLGLRRQRRTIRFVQIVLVATAAALLLFAGYA
                     WGRSAGYDAGSRSGEIDAPREPSGAQTLVLGILGLAALGGAVILQGDGTVRIPTPARL
                     DELAGRAERQAIERAEKAAETPPAESPPPPPPAPRAPAPPAAS"
     gene            2903..>3109
                     /locus_tag="VEV43_04385"
     CDS             2903..>3109
                     /locus_tag="VEV43_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22790.1"
                     /translation="MDTDEVRVALYRSFVETGRPPMPADLAAGTGASLDEVEAALHEL
                     ASEDVIALLPGTSFVWLAHPFSAAA"
BASE COUNT          410 a         1116 c         1106 g          477 t
ORIGIN      
        1 ccgccagtag gagctaggcc tcggccctcg ctgcttcgag cgcctgaacg gtcgcgaacg
       61 ccgccgacgc gtgggggtcg tccggcccca cccgggccct ctgcttgcgt gccagtcgat
      121 ccatgaccgc gagcgcaccc ggttcctcct cggcgtcgcg cagctccaca acccccgcgt
      181 cgaccgcgcg gcgtaggtag tcctctcccc tctcggaccg gacgaggatc atcgtccagc
      241 cctccatgcc gaggccgccg cacgacaggt ccgcatgctc ggcggcgaag tcggggcagt
      301 ggtggcacca ctcgttcgcg aacggacgcg cgtgcttgag cgggatgtcg acgtcgcggc
      361 cgtcgttcag ggtcaggatg accttgccct tgacgttgac cttcttgacg tcctcgagcg
      421 ggatgccgag gtcgtgcttg accttgccga ccatgaacgc gtcgtagtcg aacgtctcgc
      481 agcacatcag cccgatgacc aggccgacgc gccgggtcca ccgtttgatg ccctcggcag
      541 cgaggttgcg taccgccgtc gactcgcacg acaccccgac gaccgcgatg ttcttcagct
      601 tcatctcggc ggccttcttg agcgctagcg gcgtcgcgca gtacgtgtag cgggaccccg
      661 ccttgtcgag cagctcgtcg gggctctgca cgatcgcggg ctcgtcgagc cagggcacgt
      721 cggccgacgg cgcggagacc gcggccccgt cgagctcgcc ggtgtcgagg ccccacgaga
      781 tcagcgcggt gaccgctccc ccgtcctgtc cccgctcgag gatcggcgcg taggtcgctc
      841 gcgcgagcgt cttccaccgg tacgtgccct cgggctggtc ctggtcacgg cgtttgccgt
      901 ggacggccgt ctcgatcacg tcgatcttcg ggtcgagccg caggcacgcc atcgcgcaca
      961 gcgagcaccc ttcctggccg tggacgcagt cgtcgaacgg cgagctcagg tcggtctgga
     1021 cggggtccca gtcggagagc tcgaggacgt ggtgggggca ggccacgatg caggccgagc
     1081 agccgcagca gatctcggtc gcgacgacct cggcatagag atgggcccag tgcttgcgct
     1141 cgacgtcggc catgtccgcg gcatcgtacg gcgaacctgc gggcacatcc gtcagtctga
     1201 agggcatgaa ggccttcctc gggatcctga tcgagctcgt ggtcgccgtg acctccccgg
     1261 ctccgccgga ccgggtcgcg cccagggcgc cggcggtgaa cgtgtacctg ctgcaagagg
     1321 acgccggcgg gtaccgccgc accgcgtacc agcccctgcc gcgacctgcc cgcggtgcgc
     1381 gcggacccgg cgaacgcctc caggtcgcgc tggacgagct cgtacggggg acgacgcgga
     1441 cggagcgccg ctcgggatac acctcggcct tcacgcgcga caccgccggc gtgatccggc
     1501 gcgccgagct ccgaggtcgg cgtgccgtcg tcgacttccg ggacttccgc gacgagcagg
     1561 ggcagtgggg caccgcgtac ggaggcagcg tcttcttgat gcaggtgagc cgcacgatct
     1621 tccagttcga gcgcgtccgg tcggctctcc tcaggatcga cgggagctgc cggcagttct
     1681 gggagttcct gcagatgcgc tgcgaggtcc tgcgccggcc ttagtcgtcc aggggcgtcg
     1741 gcctgaggcg tccggcgcgg ccttcgcgca ggtggcgaat ctcacgcctg cttcctaccc
     1801 aggaacctcc cgtagggtca ccgcatggat gccgggctgc ttcccgtgaa ggcgctcggc
     1861 cgcgccaagt cccggctctc cccgctcttc gacgacgacc agcgccgagc cgtcgctacc
     1921 gcactcctcg aagacgcgct ctcgttgtgc gcgtcggccg gcttcctcga gtggtgggtc
     1981 gtcagcgagg accccgaggt cctcggccgc ggctcggggg cggggctgaa gaccgtgacc
     2041 gatccgggcg gggggctcaa cgccgcggtc gccgcgggga tcgaagaggt cgtcgccgcg
     2101 ggggccgagt ccgtgacggt catcccctgc gacgtgccgc tcgcgtacaa gggggacctc
     2161 gtcgacctgg tcgacaccgg cgcgacgtcg gacatcgtgc tggtgccctc gggcggggac
     2221 ggcggcacga acgcgctctt cctctcgccg cccggcctca tcgaaccggc cttcggcccc
     2281 ggcagcctcc agaggcacat cgcgctcgcg gagaaggagc gctaccgctg cgcgatcctc
     2341 agccttccgc gactcgccct agacgtagac acaccggacg acgtcgacgc gttcctggct
     2401 tcggcgggcg agttcggcgg gaggtccgcg gaggtgctca ggaagctgcg ggaggggcag
     2461 gcgccctagg cgccggtggc ggtggaggag gcgattcggc cggcggtgtc tcggcggcct
     2521 tctcggcccg ctcgatcgcc tgccgctccg cccggcccgc gagctcgtcg agccgcgccg
     2581 gcgtcgggat ccgaacggtc ccgtcaccct ggaggatcac cgcgcccccg agcgcggcga
     2641 ggccgaggat cccgagcacg agcgtctgcg cgccggaggg ctcgcggggc gcgtcgatct
     2701 cgcccgaacg ggagcccgcg tcgtagccgg ccgaccggcc ccacgcgtag cccgcgaaca
     2761 gcagcagggc ggcggccgtc gcgacgagga cgatctgcac gaaacggatc gtccgtcgct
     2821 ggcggcggag gccgaggggg ccgcgatgcg tgcccccggt catcgttcgt ttcatgcggg
     2881 cgacgataac gttgatcgcg ccgtggacac tgacgaggtt cgagtcgcgc tctaccggtc
     2941 gttcgtggag accggacgcc cgcccatgcc cgccgatctc gcggccggca cgggtgcctc
     3001 tctcgacgag gtcgaggcgg cgctccacga gctggcgagc gaggacgtca tcgcgctcct
     3061 ccccggtacg agcttcgtgt ggctcgcgca tcctttctcg gcagcggcc
//
LOCUS       DASQDO010000147         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_14820, whole genome shotgun sequence.
ACCESSION   DASQDO010000147 DASQDO010000000
VERSION     DASQDO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14820"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2342
                     /locus_tag="VEV43_04390"
     CDS             <1..2342
                     /locus_tag="VEV43_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYP22791.1"
                     /translation="ATDVVVGSPQAGRNRSESEGLIGFFVNTLVLRTDLSGDPTFSEL
                     LERTREVALGAYAHQDLPFEKLVEELNPERDLSHLPLAQVMLVLQSTPADRLALRGVD
                     VTPYPLTEDMAPYDVTVELRPDGEGLAGSFLYNTDLFDAETIEGLVAHFARFVSAVAR
                     EPGARIDDVALLDATEVERLAHGWVDVRPELTGSPLLHERLERTARAVPDSVAVDAPG
                     RRVTYAELDHMAKALAAKLRDAGLGREDRVGVYFDKGVELIAALCGVLRSGAAFVPLD
                     PDGPVERLRLILDDLRPAVVLAGRAESARLGDTTGAPVWTVDADELLASPGDHAAPAI
                     DAHGLAYVIYTSGSTGVPKGVMIEQSGVVGMVQSMTERYEVTSDARFLQFMSIGFDAS
                     FSEIFPTLLVGGTLVLRGRGDLLPGPGLVDLLERDRITHLLTTPSALGVMRPAGLPHL
                     RTLIVCGEACSADLVARWASGRRFVNAYGPTEVTVCTHTCHVVEDGKPPIGYGLANTE
                     SYVLDPALNETPVGVKGEMYVGSPGVARGYLGRPGLTAAAFVPNPFGPPGTRMYKTGD
                     VVRRRRDGQIDFLGRVDDQAKVRGFRIEPGEIESALALHPEVGDVAVVTDRDPAGATR
                     LVCYFTSPSGAAAQGLRDFLAERLPSYMIPAAFLRLDEMPRSPAGKLDRKALPDPRLI
                     ETDDRAAYVPPATATEKETARVWGEVLGVGRVGVRDKFFEIGGNSLKVVELFERLDEL
                     YPGALSVAELFEHPTVTAMAAAIDTRADTAAPALTGFEL"
BASE COUNT          329 a          854 c          829 g          346 t
ORIGIN      
        1 gcgccaccga cgtcgtcgtg ggctctcccc aagccggtcg caaccgttcc gagagcgagg
       61 gcctcatcgg cttcttcgtc aacaccctcg tgctgcgcac cgacctgtcg ggagacccga
      121 ccttcagcga gctgctggaa cgcaccagag aggtcgccct cggtgcctac gcccaccagg
      181 acctcccgtt cgagaagctc gtcgaggagc tgaaccccga gcgcgacctc agccacctgc
      241 cgctcgcgca ggtgatgctc gtgctccaga gcacgcccgc ggatcgcttg gcgctgcgcg
      301 gcgtcgacgt gacgccctac ccgctgaccg aggacatggc gccgtacgac gtgacggtcg
      361 agctgcggcc cgacggcgag ggcctcgcag ggtcgttcct ctacaacacc gacctcttcg
      421 acgccgagac gatcgagggc ctcgtcgcgc acttcgcacg tttcgtgagc gcggtcgcgc
      481 gcgagcccgg cgcccggatc gacgacgtcg cgctactcga cgcgacggag gtcgagcggc
      541 tggcgcacgg gtgggtggac gtgagaccgg agctgaccgg gtcgccgctg ctgcacgaac
      601 gcctcgaacg gacggcacgc gcggtcccgg actccgtcgc cgtcgacgcg ccgggccgtc
      661 gggtgaccta cgcggagctc gaccacatgg cgaaggcgct ggcggcgaag ctgcgcgacg
      721 ccgggctcgg ccgggaggac cgcgtcggcg tctacttcga caagggcgtc gagctgatcg
      781 cagcgctgtg tggcgtgctg cgctcggggg cggcattcgt gccgctcgac cccgacgggc
      841 ccgtcgaacg gctgcggctg atcctcgacg acctccgtcc ggccgtggtg ctggcggggc
      901 gggcggagag cgcacggctg ggcgacacga ccggcgcccc ggtgtggacg gtcgacgcgg
      961 acgagctgct ggcgtcgccg ggcgaccacg ccgctcccgc gatcgatgca cacggcctcg
     1021 cctacgtcat ctacacgtcc ggctccaccg gagtgcccaa gggcgtgatg atcgagcagt
     1081 ccggcgtcgt cggcatggtt cagtcgatga ccgagcgcta cgaggtgacg tcggacgcgc
     1141 gcttcctgca gttcatgtcg atcggcttcg acgcgtcctt ctccgagatc ttcccgacgt
     1201 tgctcgtcgg agggacgctc gtgctgcgcg gccgcggcga cctcctgccc ggccccggtc
     1261 tcgtcgacct cctagagcgc gaccggatca cgcaccttct gacgacacct tccgctctcg
     1321 gcgtcatgcg ccccgccggc cttccgcacc tgcgcacgct gatcgtgtgc ggcgaggcgt
     1381 gctcggccga cctggtcgct cgctgggctt cgggccgccg gttcgtcaac gcgtacgggc
     1441 cgaccgaggt caccgtctgc acccacacct gccacgtcgt cgaggacgga aaaccgccga
     1501 tcggatacgg gctggcgaac accgagtcgt acgtgctcga ccccgcgctg aacgagacgc
     1561 cggtaggggt caagggcgag atgtacgtcg gcagccccgg cgtggcgcgc ggctacctcg
     1621 ggcggccggg gctcaccgcc gccgcgttcg tgccgaaccc gttcggcccc cccggcaccc
     1681 gcatgtacaa gaccggcgac gtcgtgcgcc gccggcggga cggccagatc gacttcctcg
     1741 gccgcgtcga cgaccaggcg aaggtgcgcg gcttccggat cgagcccggc gagatcgagt
     1801 ccgcgctcgc gctgcatccc gaggtaggcg acgtcgcagt cgtaaccgac cgtgatcccg
     1861 caggggcgac gcggctcgtg tgctacttca cgtcgccgtc gggggccgcg gcgcagggcc
     1921 tgcgcgactt cctcgccgag cggctccctt cgtacatgat cccggccgcg ttcctgcgcc
     1981 tggacgagat gccgcgctcg cccgcgggga agctcgaccg caaagcgctg cccgaccccc
     2041 ggctgatcga gaccgacgac cgcgccgcgt acgtgcctcc ggccaccgct accgagaagg
     2101 agaccgcgcg cgtgtggggc gaggtcctcg gcgtgggccg ggtcggcgtg cgcgacaagt
     2161 tcttcgagat cggcgggaac tcgctgaagg tcgtcgagct cttcgagcgg ctcgacgagc
     2221 tctacccggg agcgctctcg gtggccgagc tcttcgagca tccgacggtc accgcgatgg
     2281 cggctgcgat agatacccgg gccgataccg ccgcaccggc gctgacgggg ttcgagctgt
     2341 gagctacgaa gacgtagc
//
LOCUS       DASQDO010000148         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_565694, whole genome shotgun sequence.
ACCESSION   DASQDO010000148 DASQDO010000000
VERSION     DASQDO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565694"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VEV43_04395"
     CDS             <1..429
                     /locus_tag="VEV43_04395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYP22792.1"
                     /translation="LPYRTFTVLSGSMEPTIPVGSMIFDHEVDASELARGDVVTFHPP
                     GQPDKLVSHRVVRVEETKQGSFLVTRGDANGVVDDWRIPAEGRGLRYEFHVPYLGYVV
                     GGLLTPVGRLVALSLAALWLGGLALWTIWRPRKEPLPARG"
     gene            432..1313
                     /locus_tag="VEV43_04400"
     CDS             432..1313
                     /locus_tag="VEV43_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22793.1"
                     /translation="MRRPTKGALVAGAVAAIAIAGVARGTFAAFTGTVRNGGNTFALT
                     SLYAPGNLTATPAGNAVDVAWAAGQNGNGYTVLSAPAPDPLVNDCAGASYSAVTAVSG
                     TSYTHGVSTPPGTWRCYTVRTSYHSWTSVENNPVAGARMGFVASVVSFSNTGNPRIDD
                     GDTITVTFNQAVDPATGPQTGNTICWQANRITLGSTGTGNCSATEVPRLGFLTGGAID
                     RNMRFAATWSWSADGRTLTITVGGRSVGSGNYSQSGAWTFTPTTNTNFVKSATGGIHV
                     CDTNAGGGNCTPPVSGL"
     gene            complement(1310..1450)
                     /locus_tag="VEV43_04405"
     CDS             complement(1310..1450)
                     /locus_tag="VEV43_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22794.1"
                     /translation="MRAARWARWLLAATVVFQVSASAGARLSPVETFSAVPLPPRASS
                     GR"
     gene            1626..2051
                     /locus_tag="VEV43_04410"
     CDS             1626..2051
                     /locus_tag="VEV43_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYP22795.1"
                     /translation="MRTREDGFTLIELLVVIIVIAILAAIAIPVFVNQRQKGYAAQVQ
                     SALKNASTAIEAYAADNGGDYSGLETVPDLAATLADNGFDVPSWALTFDVVAVEDNYC
                     IEIRHAEANASTGWRRGTYFGDRGAPGETPDHCPGAGAL"
     gene            2313..2819
                     /locus_tag="VEV43_04415"
     CDS             2313..2819
                     /locus_tag="VEV43_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22796.1"
                     /translation="MYHGNAQLYADRDVDKVRSRLVRTIDAVMHAQERPAYLMTACRI
                     DGRLGLFARDVYNRAPFRIQVTRAGLELSDDTYVRMTPDGMFECEGWAPFRPEYIVAK
                     NLPGPRGREHENVNKGALLAFLFGILRVGDVGMVELHRLTSTIRDARVVAEDDPEQLV
                     AALRSLPR"
BASE COUNT          456 a         1022 c         1029 g          416 t
ORIGIN      
        1 ctgccgtacc ggacgttcac ggtcctgtcc ggcagcatgg agccgacgat cccggtcggg
       61 tcgatgatct tcgaccacga ggtcgacgcg tcggagctgg cgcgtggcga cgtcgtcact
      121 ttccacccgc cgggtcagcc cgacaagctc gtgagccacc gggtcgtgcg ggtcgaggag
      181 acgaagcagg gctcgttcct cgtgacgcgc ggcgacgcga acggcgtcgt cgacgactgg
      241 cggatcccgg ccgagggccg cggtcttcgt tacgagttcc acgtgccgta cctcggctac
      301 gtcgtcgggg ggcttttgac cccggtgggc cggctggtgg ccctgtcctt ggcggcgctg
      361 tggctggggg gcctcgctct gtggacgatc tggcgcccac gcaaggagcc gctccccgcg
      421 cggggctgag catgcgaagg ccgacgaagg gcgcgctcgt cgcgggggcg gttgcagcga
      481 tcgcgatcgc cggcgtcgcg cgcggcacgt tcgctgcgtt caccgggacc gtccgcaacg
      541 gcggcaacac cttcgcgctc acgtcgctct acgcgcccgg caacctgacg gcgacgccgg
      601 ccggcaacgc ggtggacgtc gcgtgggccg cgggccagaa cggcaacggc tacacggtcc
      661 tgtccgcacc ggcgccggac ccgctcgtga acgactgcgc gggcgcgtcc tactcggccg
      721 tgacggcggt gagcgggacc tcctacaccc acggggtctc tacgcccccg gggacgtggc
      781 gttgctacac ggtccggacg agctatcact cgtggacgag cgtcgagaac aacccggtcg
      841 cgggggcccg gatggggttc gtcgcgagcg tggtgtcgtt ctcgaacacc ggcaacccga
      901 ggatcgacga cggcgacacg atcaccgtga ccttcaacca ggcggtcgac cccgccaccg
      961 gcccccagac gggtaacacg atctgctggc aggccaaccg gataacgctc gggagcacgg
     1021 ggaccggcaa ctgctcggcc accgaggtgc cgaggctcgg cttcctgacc ggcggcgcga
     1081 tagaccgcaa catgcgcttc gcggcgacgt ggtcgtggag cgccgacggc aggacgctca
     1141 cgatcaccgt cggcgggcga agcgtggggt cgggcaacta ctcgcagagc ggcgcgtgga
     1201 ccttcacgcc gacgaccaac acgaacttcg tgaagagcgc gaccggcggg atccacgtgt
     1261 gcgacaccaa cgcaggaggc ggcaactgca cgccgccggt gagcgggctc tagcggccgg
     1321 acgaggcgcg cggcgggagc ggcacggccg agaaggtctc gacggggctg agcctcgcgc
     1381 cggcggaagc ggagacctgg aacaccaccg tcgccgccag caaccaccgc gcccacctcg
     1441 ccgctctcat acctacgaac ttcgacgtag acggcgtccg gctccataga gcaccggtgt
     1501 cagatcgcgc gccggagcgg cccgtcttcg cgtggccccc cggcgcggat tgtcccggtt
     1561 tgctctcgcg caaggactag tcattccggc tcatgctccc gccgctacgc gccgataaga
     1621 gagcaatgcg cacccgggag gacggcttca cgctcatcga gctcctcgtc gtgatcatcg
     1681 tgatagcaat cctcgccgcg atcgcgatcc cggtgttcgt caaccagcgc cagaagggct
     1741 acgccgccca ggtccagtcc gcgctcaaga acgcgtcgac cgcgatcgag gcgtacgccg
     1801 ccgacaacgg cggcgactac tcgggtctgg agaccgtgcc cgacctcgcc gcgaccctcg
     1861 ccgacaacgg cttcgacgtc ccgtcgtggg cgttgacgtt cgacgtggtc gcagtcgagg
     1921 acaactactg catcgagatc cgccacgcgg aagccaacgc gtcgaccggg tggcggcggg
     1981 gaacgtactt cggggacaga ggcgctccgg gcgaaacgcc ggaccactgc ccgggtgccg
     2041 gtgcgcttta gtctcacccc gtgatcgaga tcggaaagat ccccgagaca gtcaggacct
     2101 gagcggatcc cttcggggac gacctggtct gggtcgattc gcaatgggtc cacgacggga
     2161 cggactggct ggaggcgcgg tccacgcagc cggggctgct cgccggcctg ccggtcccgt
     2221 gggacgagcc gccggaggac gccccgcgtt acgaaggcat cttcagcctg gtcgtcgacg
     2281 acccgggcaa gaagacgcgc gccctgccga cgatgtacca cggcaacgct cagctctacg
     2341 ccgaccgcga cgtggacaag gtccgctcgc gactggtccg cacgatcgac gcggtcatgc
     2401 acgcgcagga gcggcccgct tacttgatga ccgcgtgccg tatcgacggg cgtctcgggc
     2461 tgttcgcgcg cgacgtgtac aaccgcgcgc cgttccgtat ccaggtgacg cgcgccgggc
     2521 tcgagctgtc cgacgacacc tacgtgcgga tgacgcccga cggcatgttc gagtgcgagg
     2581 gctgggcccc cttccggccc gagtacatcg tcgcgaagaa cctgcccggc ccccgcggcc
     2641 gcgagcacga gaacgtgaac aagggggcgc tgctcgcgtt cctgttcggg atcctgcggg
     2701 tgggcgacgt cgggatggtc gagctccacc ggctcacttc cacgatccgc gacgcgcggg
     2761 tcgtcgcgga ggacgatccc gagcagctcg tcgccgccct gcgctcgctt cccaggtgag
     2821 catccgcgtc cgcctgctcg acgccgtcgt cgaggtacgg accgacgacg cggaggcggc
     2881 cgctctgctg tcgctgctgt gggggccgat gacgaccggc gac
//
LOCUS       DASQDO010000149         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_504761, whole genome shotgun sequence.
ACCESSION   DASQDO010000149 DASQDO010000000
VERSION     DASQDO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504761"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VEV43_04420"
     CDS             complement(<1..910)
                     /locus_tag="VEV43_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYP22797.1"
                     /translation="MPELDMQAVIMAGGEGSRLRPLTSNMPKPMLPIVKRPMMEHILL
                     LLRKHGITDVVATVQFLSSVIRNYFGDGSDLGMSLSYATEDVPLGTAGSVLGARDFLS
                     GPFLVVSGDALTDIDLEEVVDFHRSRGAAATLVLKRMKDPLEFGIVMTDDDGKIERFL
                     EKPTWGQVFSDTINTGIYVLEPEVLDLIPIDQPYDFSSELFPMMLDKGLPLFGFVTDT
                     YWTDVGNTEAYVQANYDALSGKVAVDLAGFELQPGVWVGDDVEINPTARVEGPCLIGD
                     NVRIDAGAKVGPLTVIADNAIVCDDAS"
     gene            complement(913..1515)
                     /locus_tag="VEV43_04425"
     CDS             complement(913..1515)
                     /locus_tag="VEV43_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYP22798.1"
                     /translation="MTQPTPPSAASTDRGLWTVPNVLSILRLLSVPVFVWLFVSGHEN
                     SAVILYAAGAWTDFFDGVIARKFDQVSEIGKLLDPLADRILIIALVVALVARDILPLW
                     LAIAIVGRDLLLLSVFPILERRKVPRIAVNFTGKTATASLLTGLTMLAWSETTFPGAS
                     IADELGIALTVFGAVLYWVAAAMYARAAREGIRALTTEGG"
     gene            complement(1523..1597)
                     /locus_tag="VEV43_04430"
     tRNA            complement(1523..1597)
                     /locus_tag="VEV43_04430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1560..1562),aa:Pro,seq:ggg)
     gene            complement(1683..3023)
                     /gene="der"
                     /locus_tag="VEV43_04435"
     CDS             complement(1683..3023)
                     /gene="der"
                     /locus_tag="VEV43_04435"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HYP22799.1"
                     /translation="MAEDTQRGLPLVAVVGRPNVGKSSLVNRVLGRREAIVEETPGVT
                     RDRRSFVAEWGGRRFEIVDTGGLEPGAKGLDARVAEQAEVAMEAADLIVFVVDATAGP
                     LEDDMIVAAKLRSGDKPVLVVANKVDDPRDEPAAAEFYRMGLGDPAPLSALHGRGSGD
                     FLEELVKRLPEVEETESGEWGSIAIAGRPNVGKSSILNALLREERSIVDSVPGTTRDP
                     VDSFVEHGGRLLRIVDTAGMRRRVAIGESLEYYSWLRSRQAIKRADVVLLVVDMGEGV
                     TSLDQRIAQDVVESGRACVIALNKWDLIPGEETDRARTERDIQERLRWLEWAKVVRTS
                     ALTRRGVDRILPALVDALESHRRRLPTAHVNRIVTEAQARRPHPRAGHRAVRVLYAVQ
                     ASAAPPEIVLFSTGQLTPGYLKYLEHRIRDAEPFEGTPVKLRTRIRRRKGESAS"
     gene            complement(3016..>3203)
                     /locus_tag="VEV43_04440"
     CDS             complement(3016..>3203)
                     /locus_tag="VEV43_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYP22800.1"
                     /translation="PQDDSKLAAMERELSRRDTADATRDVAPLTRPEGAHEVDTTGLT
                     LQEVVERICSIVGEHRG"
BASE COUNT          482 a         1079 c         1137 g          505 t
ORIGIN      
        1 tcgaggcgtc gtcacagacg atcgcgttgt cggcgatgac ggtgagcggg ccgaccttgg
       61 cccccgcgtc gatccggacg ttgtcgccga tcaggcaggg accctcgacc cgggccgtgg
      121 gattgatctc gacgtcgtcg cccacccata cgccgggctg gagctcgaag cccgccaggt
      181 ccactgcgac cttcccggac agcgcgtcgt agttcgcctg cacgtacgcc tcggtgttgc
      241 cgacgtcggt ccagtacgtg tcggtgacga acccgaacag cggcagtccc ttgtcgagca
      301 tcatcgggaa cagctcgctc gagaagtcgt acggctggtc gatcgggatg aggtcgagga
      361 cctcgggctc gagcacgtat atgccggtgt tgatcgtgtc ggagaagacc tggccccacg
      421 tcggcttctc gaggaagcgc tcgatcttgc cgtcgtcgtc ggtcatcacg atgccgaact
      481 cgagcgggtc cttcatgcgc ttgaggacga gcgtcgcggc ggcgccgcgg ctccggtgga
      541 agtcgacgac ctcctccagg tcgatgtcgg tgagcgcgtc gccggagacg accaggaacg
      601 ggcccgagag gaagtcgcgc gcgccgagga cggacccggc ggtaccgagc ggcacgtcct
      661 cggtcgcgta ggacagcgac atcccgaggt cggagccgtc tccgaagtag ttgcggatga
      721 ccgacgagag gaactggacc gttgcgacta cgtccgtgat cccgtgcttg cgcagcagca
      781 ggaggatgtg ctccatcatc gggcgcttga cgatcgggag catgggcttc ggcatgttgc
      841 tcgtcagggg gcgcaggcgc gacccctcgc cgccggccat gatcaccgcc tgcatgtcga
      901 gctcgggcac tgctaacccc cctccgtcgt cagtgcgcgg atcccctcac gtgcggcccg
      961 tgcgtacatg gccgcggcga cccagtagag cacggcgccg aacacggtga gggcgatacc
     1021 caactcgtcg gcgatgcttg caccggggaa cgtcgtctcc gaccacgcga gcatcgtcag
     1081 gccggtgagg agactcgcgg tcgcggtctt ccccgtgaag ttcaccgcga tgcgcgggac
     1141 cttgcggcgc tcgaggatcg ggaacacgct gagcaacagg aggtcgcgcc ccacgatcgc
     1201 gatcgcgagc cacagcggga gaatgtccct ggctacgagc gcgacgacga gagcgatgat
     1261 caggatgcga tcggccaggg ggtcgagcag cttgccgatc tcggagacct ggtcgaactt
     1321 ccgagcgatc acgccgtcga agaagtcggt ccacgcgccg gccgcgtaca ggatgacggc
     1381 gctgttctcg tgaccggaga cgaagagcca cacgaagacg gggacgctga gcaggcggag
     1441 gatcgagagg acgttcggga cggtccacag gccgcgatcg gtcgacgcgg cggatggtgg
     1501 agtcggctgt gtcatctatg agtcgggacg gccggatttg aaccggcgac ccctggtccc
     1561 ccagaccagt gcgctaacca agctgcgcca cgtcccgtga tcttcctgcg gtcatcatac
     1621 ccagggggtt cgcgcgggct tttgtccttg gagcatcgcc gaggcgatgc ttcggggcgt
     1681 cgtcagctcg cgctctcgcc tttgcgcctg cggatgcgcg tgcgcagctt caccggcgtc
     1741 ccctcgaacg gctcggcgtc gcggatgcgg tgctcgaggt acttgaggta gcccggcgtc
     1801 agctgcccgg tcgagaacag gacgatctcc gggggggccg cgctcgcctg caccgcgtac
     1861 aggacgcgga cagcgcggtg gcccgcgcgg ggatggggcc ggcgcgcttg cgcttccgtg
     1921 acgatgcggt tcacgtgtgc cgtcggcaat ctccgccggt gggattccag cgcgtcgacg
     1981 agggccggca ggatgcggtc gacgccgcgg cgggtcagcg cggaggtgcg cacgaccttc
     2041 gcccactcga gccaccgcag acgttcctgg atgtcgcgct ccgtgcgcgc gcggtcggtc
     2101 tcctcgccgg ggatcaggtc ccacttgttg agcgcgatga cgcaggcgcg gccggactcg
     2161 acgacgtctt gcgcgatgcg ctggtcgagc gacgtgacgc cctcacccat gtcgacgacg
     2221 aggagcacga cgtccgctcg ctttatcgcc tggcgcgagc ggagccagct gtagtactcg
     2281 agcgactcgc cgatcgcgac gcgcctccgc attcccgcgg tgtcgacgat gcgcagcagc
     2341 cggcccccgt gctcgacgaa ggaatccacg ggatcgcgcg tcgtgcccgg aaccgagtcg
     2401 acgatcgacc gttcctcgcg cagcagcgcg ttgaggatcg acgacttgcc tacgttcggg
     2461 cgtcccgcga tggctatcga gccccactcg ccggactccg tctcctccac ctccggcagg
     2521 cgcttgacga gctcttcgag gaagtccccg gagccgcggc cgtgcagggc cgagagcggg
     2581 gcggggtcgc cgaggcccat ccggtagaac tcggcggccg cgggctcgtc gcgggggtcg
     2641 tcgaccttgt tcgcgaccac gaggacgggc ttgtcgccgc tgcggagctt cgccgccacg
     2701 atcatgtcgt cctcgagagg gccggcggtc gcgtccacga cgaagacgat caggtcggcc
     2761 gcctccatcg cgacctccgc ctgctcggcc acccgcgcgt cgaggccctt ggcacccggc
     2821 tcgagcccac cggtgtcgac gatctcgaag cgccggcccc cccactccgc gacgaacgac
     2881 ctgcggtcgc gcgtcacacc gggggtctcc tcgacgatcg cctcgcggcg gccgaggacg
     2941 cggttgacga gcgacgactt gccgacgttg gggcggccta ccacagcgac gagggggagg
     3001 ccccgctggg tgtcctcagc cacggtgctc ccccacgatc gagcagatgc gttcgacgac
     3061 ctcttggagg gtgagcccgg tcgtgtcgac ctcgtgcgcg ccctccggcc gcgtgagcgg
     3121 ggcgacgtcg cgggtcgcgt ccgcggtgtc gcggcgcgag agctcgcgct ccatggccgc
     3181 gagcttcgag tcgtcttgcg gaa
//
LOCUS       DASQDO010000150         4633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_627530, whole genome shotgun sequence.
ACCESSION   DASQDO010000150 DASQDO010000000
VERSION     DASQDO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627530"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..574)
                     /locus_tag="VEV43_04445"
     CDS             complement(110..574)
                     /locus_tag="VEV43_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HYP22801.1"
                     /translation="MVVEAQLERPVRGSWSVAARCHLGVPTVIESHPLMEDGSPFPTT
                     FWLTCPILVKRASRLEAAGVMTEMTERLARDEALQARLADAVARYRARRDEHLVIKES
                     GGPPGGGPDRVKCLHAHVAHELADPPNPIGALTLASTEWPDCVEPCVVAVQS"
     gene            complement(604..951)
                     /locus_tag="VEV43_04450"
                     /pseudo
     CDS             complement(604..951)
                     /locus_tag="VEV43_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
     gene            complement(988..1380)
                     /locus_tag="VEV43_04455"
     CDS             complement(988..1380)
                     /locus_tag="VEV43_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HYP22802.1"
                     /translation="MSKARRGPKFAGPGLVVFLLVLGLLASMAIEPTLELMQQNDRIA
                     RMKSELRAIETTNERLERRIARLDDPDFLEQKAREQIGLVRPDETTYLVVPPGRSRPH
                     KKKEREPAPPPPPEPGFIDGLLEFVGLS"
     gene            complement(1377..2663)
                     /gene="eno"
                     /locus_tag="VEV43_04460"
     CDS             complement(1377..2663)
                     /gene="eno"
                     /locus_tag="VEV43_04460"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYP22803.1"
                     /translation="MSEIVLCRAREVLDSRGNPTVEVEVALDSGASGRAMVPSGASTG
                     AGEALELRDGGDRYGGKGVQKAVENVNDRIGPTLLGSDALDQRLIDTMMRDLDGTDDK
                     SGLGANAMLGASLAVAHAAADELGVALFRYLGGPDAHLLPVPMMNVLNGGAHADNSVD
                     LQEFMLAPVGAATFAEALEWGSRTYHALKKLLDSKGLSTGVGDEGGFAPDLGANSEAV
                     ELLLDAIERAGFTPGTDIALALDPATSEIRDGDTYFLASEDRRLSSEEMVGFWADWAD
                     RYPIVSIEDGMAEDDWDGWVALTERIGDRVQLVGDDLFVTNPEVLQRGIDAGAANAIL
                     IKPNQIGTLTETLECVRIAHRAGYETMISHRSGETEDATIADVAVGTNAGQIKTGAPA
                     RGERTAKYNQLLRIEDALGDAARYAGRGAFRRSSAG"
     gene            complement(2707..3678)
                     /locus_tag="VEV43_04465"
     CDS             complement(2707..3678)
                     /locus_tag="VEV43_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HYP22804.1"
                     /translation="MPERLVVVPLAPGEAGWLTLEEWDLLVSCDRVVFEHPDHPLVDR
                     LRAQGVAAGPFDDEPDAGSAGWALVADPGSPRVVELAHAGAVVTSGPASAPDELTAAR
                     GARLGRRAGSSLGALALVMSRLRGPDGCPWDAEQTHESLRVHLLEEAHEVLEAIDAGT
                     TGAELEEELGDLLLQVFFHAELASEEARFDVATVADRLVAKLVHRHPHVFGDVVVAGA
                     GEVVSNWEAIKAEEKRDVRADPFDGIPPTLPALLKTYKVQKKAARLGFEANAGAARAG
                     IEKALAEEPSPGSIGELLFWTVALARSAGVDPEGALARATLKFRQSL"
     gene            complement(3681..>4633)
                     /locus_tag="VEV43_04470"
     CDS             complement(3681..>4633)
                     /locus_tag="VEV43_04470"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYP22805.1"
                     /translation="VERQLDDYVASRSFEQQAAQGDAGALKRQFEQDRLTDLIMRAVL
                     TPAAEERGIEVTTADVVDRIEEFVEAEFAGSQAQLDEALNENGLTQAQFHEIIHDQIL
                     DESLRADVAGDVRPSGEDVRAFYEENRAEFETARAQHILVDDRALAEQLAARLQAASG
                     EAAETLFEKLAREHSTDPSSARNGGDLGFQPAGTFVGPFEEAMAGLDVGEVSDPVRTE
                     FGWHVIRVIDRRVTPLEEARAQIEDQLAGPAQDEAWNSFLDELFEEAEVEVNPRYGVF
                     DEETRRVVDPDADDIPGGEAPEPSAEPSAPQFPAPPPPPG"
BASE COUNT          605 a         1692 c         1597 g          739 t
ORIGIN      
        1 ccgagccgcg tgatcgtgag ccgtcgctcg agcgcacgga agccgtcggc gagctcctcc
       61 gcgacgagga gccgcgtgga cttggtaccg acgtcgatag ccgcaactct cacgactgaa
      121 ctgccaccac gcagggctcg acacagtcgg gccattcggt agacgcgagc gtcagcgccc
      181 ctatcgggtt cggcgggtcg gcgagctcgt gcgctacgtg cgcgtgcagg cacttcacgc
      241 ggtccggccc ccctcccggc gggcctcccg actccttgat caccagatgc tcgtctcgtc
      301 gcgcccggta gcgcgcgacg gcatcggcga gacgcgcttg caacgcctcg tcacgggcca
      361 gcctctcggt catctccgtc atcacgcctg ccgcttcgag gcggctcgct cgcttcacga
      421 ggatcgggca cgtcagccag aacgtcgtcg ggaacggcga cccgtcctcc atgagtggat
      481 ggctctcgat caccgtcggg accccgaggt ggcagcgcgc ggcgaccgac cacgatcctc
      541 gtacgggacg ttcgagctga gcctcgacga ccacgcggtc ctcgggcgag tgacgcagcg
      601 acgctacttc ttgccgcctt ccttgtgccg cttgaggtcg accaggcgct cctggctctg
      661 gtgcatgaac ttcttcagct tctgctcgaa ctcggggtcg cggccgccct tgcgcgcggg
      721 gcgctcctcg tagccgggct cggcctgctt gatcgagagg tcgatcttgc cgtcctcttt
      781 ctggccgacg accttgacgg tcaccttgtc gccctcgcgg atgtgctcgc ggatgtcgcg
      841 cacgtagttc ttgtcgacct gcgagatgtg gaccattccg ctctcgccgg aatcgagctc
      901 tacgaacgcg ccgtagtcca tgaccttctg gaccgtgcct tcgacgattg aaccgacctc
      961 gactgccctc ccggtaagcg taccccgtca ggacaagccg acaaattcga gcaaaccgtc
     1021 tatgaacccc ggctcgggcg gcgggggcgg ggcgggctcc cgctccttct tcttgtgcgg
     1081 gcgggaacgg cccggcggca cgaccaggta ggtggtctcg tcggggcgga cgaggccgat
     1141 ctgctcgcgg gccttctgct ccaggaagtc cgggtcgtcg aggcgggcga tcctccgctc
     1201 gagcctctcg ttcgtcgtct cgatcgccct cagctcggac ttcatccggg cgatccggtc
     1261 gttctgctgc atgagctcga gcgtgggctc gatcgccatc gacgcgagga ggccgagcac
     1321 gagcaggaac acgacgagcc ccggcccggc gaacttcggc cctctccgcg ccttgctcat
     1381 cccgccgacg acctccggaa cgcgcctcgt cccgcgtagc gggcggcgtc gccgagcgcg
     1441 tcctcgatcc tcaggagctg gttgtacttc gcggtccgct cgccccgggc gggggccccg
     1501 gtcttgatct ggccggcgtt cgtccccacc gcgacgtcgg cgatcgtcgc gtcctcggtc
     1561 tcgccggaac ggtgggagat catcgtctcg tagccggcgc ggtgagcgat gcggacgcac
     1621 tcgagcgtct cggtgagcgt cccgatctgg ttcggcttga tcaagatcgc gttcgccgcg
     1681 cccgcgtcga tgccgcgctg cagcacctcg gggttcgtca cgaagaggtc gtcgccgacg
     1741 agctggacgc gatccccgat gcgctcggtg agcgcgaccc agccgtccca gtcgtcctcg
     1801 gccatcccgt cctcgatcga cacgatcggg tagcgatcgg cccagtcggc ccagaacccc
     1861 accatctcct cgctcgacag cctccggtcc tccgaggcga ggaagtacgt gtccccgtcg
     1921 cggatctccg acgtggcggg gtcgagcgcg agcgcgatgt cggtccccgg cgtgaagccg
     1981 gcgcgctcga tcgcgtctag cagtagctcg actgcttccg agttcgcgcc gaggtcgggc
     2041 gcgaagccgc cctcgtcgcc gacgcccgtc gagaggccct tcgagtcgag gagcttcttc
     2101 agcgcgtggt acgtccggct cccccattcg agggcttccg cgaacgtcgc ggcgccgacc
     2161 ggcgccagca tgaactcctg caggtcgacg gagttgtcgg cgtgtgcccc tccgttgagg
     2221 acgttcatca tcgggaccgg cagcaggtgg gcgtccggcc ccccgaggta gcggaacaac
     2281 gcgacaccga gctcgtccgc cgccgcgtgc gcgaccgcca gcgacgcgcc cagcatggcg
     2341 ttcgccccga gccccgactt gtcgtcggtc ccgtcgaggt cccgcatcat cgtgtcgatc
     2401 aggcgctggt cgagggcgtc gctgccgagc agcgtcggcc cgatccggtc gttcacgttc
     2461 tcgaccgctt tctggacgcc cttgcccccg tagcggtcgc cgccgtcccg cagctcgagg
     2521 gcctcgccgg ccccggtcga ggcccccgac gggaccatcg cgcggcccga cgcccccgag
     2581 tcgagggcga cctcgacctc gacggtggga ttgccccggg agtcgaggac ttcccgggcg
     2641 cgacagagca cgatttcact cacagtggtt gactcctttc acagccgtaa caacttagcg
     2701 caggagctac agggactggc ggaacttcag ggtggcgcgc gccagggcgc cctcggggtc
     2761 gacgccggcc gagcgggcga gcgccaccgt ccagaacagg agctcgccga tcgagcccgg
     2821 ggagggctcc tcggccagcg ccttctcgat cccggcccgg gcggcgcccg cgttcgcctc
     2881 gaacccgagc cgggccgcct tcttctggac cttgtaggtc ttcagcaggg ccgggagcgt
     2941 cggggggatc ccgtcgaagg ggtcggcccg gacgtcgcgc ttctcctccg ccttgatcgc
     3001 ctcccagttc gagacgacct cgccggcgcc cgccacgacg acgtcgccga agacgtgggg
     3061 atggcggtgg acgagcttcg ccaccaagcg gtcggccacg gtcgccacgt cgaagcgcgc
     3121 ctcctcggac gccagctcgg catggaagaa gacctgcagc aggaggtcgc cgagctcctc
     3181 ttcgagctcg gcccccgtcg tgccggcgtc gatcgcctcg aggacctcgt gcgcctcctc
     3241 cagcaggtgg acgcgcagcg actcgtgcgt ctgctcggca tcccaggggc agccgtcggg
     3301 accgcgcagc cgcgacatca cgagggcaag ggctcccagc gagctgccgg cacggcggcc
     3361 gagccgcgcg ccgcgggccg cggtgagctc gtcgggggcg gaggccgggc cggacgtcac
     3421 gacggcgccg gcatgggcga gctcgacgac gcggggcgag ccggggtcgg ccaccagggc
     3481 ccagccggca ctacccgcgt ccggctcgtc gtcgaacggt cccgcggcga ctccctgggc
     3541 tcggaggcgg tcgaccagcg ggtggtcggg gtgctcgaac acgacgcgat cgcacgagac
     3601 gagcaggtcc cactcctcga gcgtgagcca cccggcctcg ccgggcgcga gcgggacgac
     3661 gacgagccgc tccggcactg ctagccgggc ggtggcgggg gggccgggaa ctgcggcgcg
     3721 ctcggctcgg cgctcggctc cggggcctcg ccgccgggga tgtcgtcggc gtcggggtcg
     3781 acgacccgcc gggtctcctc gtcgaagacg ccgtagcgcg ggttgacctc gacctcggcc
     3841 tcctcgaaca gctcgtcgag gaaagagttc cacgcctcgt cctgcgcggg gcctgcgagc
     3901 tggtcctcga tctgcgcgcg ggcctcttcg agcggcgtca cccgccggtc gatcacgcgg
     3961 atgacgtgcc acccgaactc ggtcctcacg ggatccgaga cctccccgac gtcgaggccc
     4021 gccatcgcct cctcgaacgg cccgacgaac gtcccggcgg gttggaaccc gaggtcaccg
     4081 ccgttgcggg ccgagctcgg gtcggtcgag tgctcgcgcg cgagcttctc gaacagcgtc
     4141 tcggccgcct cgcccgacgc ggcctgcagc cgcgccgcga gctgctcggc gagagcgcgg
     4201 tcgtcgacga ggatgtgctg ggcgcgcgcg gtctcgaact cggcgcggtt ctcctcgtag
     4261 aacgcgcgca cgtcctcgcc cgagggccgg acgtcgcccg ccacgtcggc gcgcaacgac
     4321 tcgtcgagga tctggtcgtg gatgatctcg tggaactgcg cctgggtgag cccgttctcg
     4381 ttcagcgcct cgtcgagctg ggcctggctc cccgcgaact cggcttcgac gaactcttcg
     4441 atccggtcga cgacgtctgc ggtcgtcacc tcgatccccc gctcctcggc ggcgggcgtg
     4501 agcaccgctc gcatgatgag gtcggtcagc cggtcctgct cgaactggcg cttgagcgcg
     4561 ccggcgtcgc cctgcgcggc ctgttgctcg aagctgcggc tcgcgacgta gtcgtcgagc
     4621 tgtcgctcga ccg
//
LOCUS       DASQDO010000151         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_260096, whole genome shotgun sequence.
ACCESSION   DASQDO010000151 DASQDO010000000
VERSION     DASQDO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260096"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VEV43_04475"
     CDS             complement(<1..816)
                     /locus_tag="VEV43_04475"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22806.1"
                     /translation="MSSVDSAPSPPDRVSVVPLVPAWRVDRAYTYSVPEKLRGKLSTG
                     SLVRVPFGGRNVRAVVVADEAADTADTLLDIKQLIVEDPVAPPPLDRLVAWIADRYTT
                     PRAICLSRTIPPRVRVKVPPAEPLAGGPAPTDVTRLQGGAELVGAITRGEAGTWVVRP
                     IASQDRGALIAELVAAAAPGAALVAVPEVRYGAPTLETLTKEWPDAARVDSSEEDADR
                     ARGWLRLAAGHGLGLGGRAVVLAPAPRLRLIVVDEEHHVSYKEDRSPRYDARRV"
     gene            complement(791..1987)
                     /gene="metK"
                     /locus_tag="VEV43_04480"
     CDS             complement(791..1987)
                     /gene="metK"
                     /locus_tag="VEV43_04480"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HYP22807.1"
                     /translation="MTTQLHTFTSESVTEGHPDKMADQISDSILDEILASDPFGRVAC
                     ETLVTTGLVVVAGEISTSCYVDIPSVVRRTVTDIGYTSSKIGFDGETCGVSVAIQEQS
                     SDIDKGVSQSYEERHGETEDEALDSQGAGDQGMMFGYACNENDDLMPMPIWLAHRLAQ
                     RLSEVRKAGVLPYLRPDGKVQVTIGYDGNKPVRLDTVVCSTQHQPNVDVETLLKPDLL
                     QHVVEPLMPPGLDADGMNFLVNPTGVFEIGGPRADTGLTGRKIIIDSYGGMARHGGGA
                     FSGKDPTKVDRSAAYAGRYVAKNVVAAGLAERFEMQVAYAIGVARPVSIAIDTFGTEN
                     VDPAKILGGIKECFDLRPAAIIRDLDLRRPIYRATSAYGHFGRSEKDFTWERTDRADE
                     LRRLCS"
BASE COUNT          320 a          769 c          867 g          365 t
ORIGIN      
        1 gacccggcgc gcgtcgtagc ggggcgagcg gtcctccttg tacgagacgt ggtgctcctc
       61 gtcgacgacg atcagccgca gccggggcgc cggcgcgagg acgacggcgc gacccccgag
      121 accgagcccg tgaccggccg cgagccgcag ccacccccgg gcgcgatccg cgtcctcctc
      181 ggacgagtcg acgcgtgccg cgtccggcca ctccttcgtc agggtctcga gggtcggcgc
      241 gccgtatcgc acctcgggta cggcgacgag cgccgccccg ggggccgcgg ccgcgacgag
      301 ctcggcgatc agcgcgccgc ggtcctgcga ggcgatcgga cgaacgaccc acgtgcccgc
      361 ctctccgcgg gtgatcgcgc cgacgagctc ggccccgccc tgcaggcgtg tcacgtccgt
      421 gggggccggg ccgccggcga ggggctcggc cggcgggacc ttcacccgga cccgcggcgg
      481 gatcgtgcgc gacagacaga tcgcccgcgg cgtcgtgtag cgatcggcga tccatgcgac
      541 cagccggtcg agcggaggcg gcgcgacggg gtcctcgacg atcagctgct tgatgtcgag
      601 cagcgtgtcg gccgtgtcgg cagcctcgtc cgcgacgacc accgcccgga cgttgcggcc
      661 tccgaacggc acccggacga gcgacccggt cgagagcttc ccgcgcagct tctcgggaac
      721 ggagtacgtg taggcgcggt cgacccgcca ggccgggacg agcggtacta ccgagactct
      781 gtccgggggg ctaggagcag agtcgacgga gctcatccgc ccggtcggtg cgctcccacg
      841 tgaagtcctt ctcggaccgg ccgaagtgcc cgtatgccga agtcgcgcgg tagatcggac
      901 ggcgcaggtc gaggtcccgg atgatcgccg cggggcggag gtcgaagcat tcttttatgc
      961 cgccgaggat cttcgccggg tcgacgttct cggtcccgaa cgtatcgatc gcgatcgaga
     1021 ccggccgggc gacgccgatc gcgtacgcga cctgcatctc gaagcgctcg gcgagaccgg
     1081 ccgcgacgac gttcttggcg acgtaacggc ctgcgtacgc ggccgagcgg tcgaccttcg
     1141 tcgggtcctt gccggagaac gctcctccgc cgtgccgcgc catgcccccg tagctgtcga
     1201 tgatgatctt gcgtccggtg agcccggtgt cggcgcgggg gccgccgatc tcgaagacgc
     1261 cggtggggtt gacgaggaag ttcatgccgt ccgcgtcgag gcccggcggc atcagcggct
     1321 cgacgacgtg ctggaggagg tcgggcttca gcagcgtctc gacgtcgacg ttcggctggt
     1381 gctgcgtcga gcagacgacg gtgtcgagcc tcaccggctt gttgccgtcg tagccgatcg
     1441 tgacctggac cttgccgtcg ggccggaggt aagggagcac gccggccttg cgtacctcgg
     1501 agagccgctg ggcgaggcga tgcgcgagcc atatcggcat cggcatgagg tcgtcgttct
     1561 cgttgcaggc gtacccgaac atcatcccct ggtcgccggc gccctggctg tcgagggcct
     1621 cgtcctcggt ctcgccgtgg cgttcctcgt agctctggct gacgcccttg tcgatgtcgc
     1681 tcgactgctc ctggatcgcg acgctgacgc cgcacgtctc gccgtcgaag ccgatcttgc
     1741 tggacgtgta gccgatgtcg gtgacggtgc gccggacgac cgaggggatg tcgacgtagc
     1801 acgaggtcga gatctcgccg gcgacgacga cgagcccggt ggtgacgagg gtctcgcagg
     1861 cgacgcggcc gaacgggtcc gaggcgagga tctcgtcgag gatcgagtcc gagatctggt
     1921 cggccatctt gtcggggtgg ccctcggtga ccgactcgga cgtaaaggtg tggagttggg
     1981 tggtcatcgg ccgatcctat agccggggtg tgacaggttc cgggggccgc tctcgccgcg
     2041 tgcgcaacca atggcgactt gcgcacctga ttgcgcacgc gggggccggg gtccgcctgg
     2101 cgttctgtgc cacatgcgga acggcattcg tgctccatgt ggcacagaac gtgcgggggc
     2161 cggggccggg gcgccgtcgg ccctgcgcat ggatcccaca gcggtcgtga ccgcggcact
     2221 gtcccgtcgt aatcagcatt gggggatgcg cgagatcgca tcgagaggac gggaggattg
     2281 ccgtgagcac agtacggctc agaatggcgg cggcggctgt t
//
LOCUS       DASQDO010000152         8308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_627723, whole genome shotgun sequence.
ACCESSION   DASQDO010000152 DASQDO010000000
VERSION     DASQDO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627723"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3612
                     /locus_tag="VEV43_04485"
     CDS             <1..3612
                     /locus_tag="VEV43_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYP22808.1"
                     /translation="EWLRARLPEHLVPAFVEGLDVLPKTPGGKVDRAALAARAPQAGP
                     GSAAAPRTPTEQVLASMWQKLLGIDAAGVDESFFDLGGHSLVATQLVSRIRHAFGVSL
                     PLRTVFEWPTIAGLAAVIERSADGDVDELPLTPIELVEPVPLSFAQQRLWFIDQFKPG
                     SADYVIAARAELEGPLDVDALEYALKALARRHEALRTTFPAHGGEARQAISPDPVVVF
                     ETFDLTREPTAAAADLVQAQAARPFDLSAGPLWRTALIRLEEKRHVFVTCMHHIVSDG
                     WSLGILASELSELYSARVEAREPDLPELEVSYADFAVWQRRWFAGDELTRQLSYWKER
                     LEGAPAVLELPTDRPRPPIQSFEGATVGFTLPKDLSDEIAKLARAHEVTTFMVTLAAF
                     ELLLHRYTGATDIVVGSPIAGRNRSEIEGLIGFFVNTLVLRTDLSGDPTFAELLERVR
                     EVALGAYAHQDLPFEKLVEELGIDRDLAHSPAVQVMFVFQNTPPGDLALAGTNRARLD
                     APTNEVAVDVTLDVTETEAGLVAQAVYNADLFDRRTMEAFLRHFGNVLEAVARDAETP
                     LSRVELLDPDERATIVTGFNPPPTPFPDDVRVHEVFERVADERGNAPAVVYGDETLSY
                     TELDVRANRLARHLAAHGVGRGDLVAVVLERGIDLVISLLAILKAGAAYLPIDPENPR
                     RRTAFMLEDSEARVAVTGGTTPAGGLAAATVISLDGDAAAIAAHDASAPAVPGEAADV
                     AYAIYTSGSTGTPKGICIPHRAIARLVLDTDYVKLGPHSVVAQLANASFDAATFEIWG
                     PLLNGGVVAGIEKDVALDAGRLREALRAHGVDTAFLTTALFNQLVAEDPSLFSSLDTL
                     LVGGEAHDPSMVRRCLDAEPPAKLCNVYGPTECTTFSTWYPIESVPKGATSIPIGRPI
                     ANTKVHVLDSAMNPLPVGVPGEVHVGGPGLAAGYLDRPELTKERFVPDPFASDDPSAR
                     LYKTGDLGRYLPSGDVEFLARMDSQVKIRGFRVELGEVEAALAEHPHVKESVVTVAEA
                     RGDKRLVAYFAGDGVEVAELRRFVSERVPFYMVPSEYVELDALPLNANGKVDRRALPA
                     PDPSRHSAGEERVAPRNETESKLASIWEELLSKRPVGVHDGFFELGGHSLLATQLVSR
                     IRDAFYVELELRAVFEEPTIARLAPRVDAAPRADSPGIPELKALPRHSSRLDGSSNR"
     gene            3609..>8308
                     /locus_tag="VEV43_04490"
     CDS             3609..>8308
                     /locus_tag="VEV43_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYP22809.1"
                     /translation="MSADRKRLLELMLKQRGLAGALTDGPVPLGPDDERLLSFGQQRL
                     WFFDEWQPNGSVYNVPGAIELTGPLDVDALQHALDTISARHDSLRMTFHSAGPEPRLS
                     VAPPAPVDLPLTDLGDVPEAERYERAIALANEIALRPFDLSAGPLWRTALIRLEEDRH
                     VFVTCMHQIVSDGWSLGILASELSELYSARIEGREPDLPELEVSYADFAVWQRRWFSG
                     DELTRQLSYWKERLEGAPAVLELPTDRPRPPIQSFEGATVGFTLPKELSDEIAKLARA
                     HEVTTFMVTLAAFELLLHRYTGATDIVVGSPIAGRNRSEIEGLIGFFVNTLVLRTDLS
                     GDPTFAELLERVREVALGAYAHQDLPFEKLVEELRPARDLSISPVFQVSFALQDVRAA
                     DFELTGLEVGFTRVKDESAKVDLSLFLFEGDEGLFGELNYSTALFEETTIRSMIAHFE
                     AVLAAVTAEPAVPVSRHALISDEEGRDLVEELNPPLPAAPAGPLVHERLAELARSHPD
                     DVALVAGNASLTFGELDRRAEAVAASLDAGPGDVVAVLMERSLEQVVAQLGILKAGAA
                     YLPLDPAYPPKRLSLMIEDARPKAVISSLEDLPSPGPRRDAAVNGEDLAYVIYTSGST
                     GTPKGVGVTHANLANLLEWDRGQYLGPGRTTSLAPLSADASVWELWPPLAAGMPVVLI
                     DRETARDPAALVRAFERHRVTNGFVPTATAHVLLRAEAPGGLETLMTAGERLTAAPDE
                     TFAATVVNLYGPTEATVAATAGIVAPGGGIPDIGTPVNGVRVYVLDEHLRPVPRGLPG
                     EVYIGGRGVASHYLNAPGLTARAFVPDPFSSEPGARMYRSGDRARHRPDGTIDFLGRG
                     DEQVKVRGYRVEPAEVEAALRTHPAVSECAVGTVVDKSGATSLVAYFVAAPEPPVSEL
                     RYYLSDLLPAHMVPSSFVTLEALPLTPAGKVDRAALPAPGAERPDVGHAYEEPRTETE
                     KVLAEIWADVLGVERVGRTDNFFDLGGHSLLGTQLMSRIRNRFEAEVPLRALFERPTV
                     GGLAATLDTTSRTESLPLVAAERGDLPVLSFAQQRMWFIDQIDPASSTYNVPVALRLT
                     GRVDGARLERALNAIVERHETLRTTFTTVRGEPRQVVADEGRVSLSVVDLAYLGDDER
                     EEAAMKVVAEEADRPFDLAAGPLWRTTLIELGDEDHLLVICMHHIVSDGWSLGVFASE
                     LSEIYTAFVDDREPKLEPLPLQYADFAIWQREWFQGEELDRQMTYWKRHLEGAPAALD
                     LPTDYPRPPIQTFEGASINFALPAGLSADVKELARRNGVTMFMLGLAAFTTLLHRYTG
                     AAEVVVGTPIAGRNRSEIEGLIGFFVNTLVMRTDLSGDPTFSELTERVRDVALGAYAH
                     QDLPFEKLVEEMKPPRDLSHSPLFQVAFGYQNTPAPEFELTGLGIDLPELDDTSAKFD
                     LTLDMGETEEGLGGSLNYATALFERTTMEEMLAHLETLLRAAVADPETRLSELPILTP
                     EERDVVVHRFNDTGAEHDADKLMHEFFEEHARSRPDAVAVRFEGGDFSYGELNERANR
                     LAHHLRSLGLRPGSR"
BASE COUNT         1217 a         3044 c         2810 g         1237 t
ORIGIN      
        1 gagtggttgc gcgcacgcct tcccgagcac ctcgtcccgg ccttcgtgga agggctcgac
       61 gtcctcccga agaccccggg gggcaaggtc gaccgtgccg ctctcgccgc gcgcgcaccg
      121 caggccgggc ccggttcggc cgccgccccc cgcacgccca ccgagcaggt cctcgcgtcg
      181 atgtggcaga agctcctcgg catcgacgcg gccggcgtcg acgaaagctt cttcgacctc
      241 ggcggccact cgctggtcgc gacgcagctc gtctcgcgca tccgtcacgc cttcggcgtc
      301 tcgctgccgc tgcggacggt cttcgaatgg ccgacgatcg ccgggctcgc cgcagtgatc
      361 gaacgctcgg ccgacggaga cgtcgacgag ctccccctga cgccgatcga gctggtggag
      421 ccggtgccgc tgtcgttcgc gcagcagcgg ctctggttca tagaccagtt caaacccggg
      481 agcgccgact acgtgatcgc cgcgcgggcg gagctcgagg gaccgctgga cgtcgacgcg
      541 ctggagtacg cgctgaaggc gctcgcccgg cggcacgagg ccttgcgcac gacgttcccg
      601 gcgcacggag gagaggctcg ccaggcgatc tcgccggacc ccgtcgtcgt cttcgagacg
      661 ttcgatctga cccgcgagcc caccgctgcc gcagcggacc tcgtgcaagc gcaagccgcg
      721 cggcccttcg acctctccgc cggccccctg tggcgcaccg cgctcatccg cctcgaagag
      781 aagcgccacg tcttcgtgac gtgcatgcac cacatcgtgt cggacggctg gagcctcggc
      841 atcctcgcct cggagctctc ggagctctac tcggcaagag tcgaagcacg cgagcccgac
      901 ctcccggagc tcgaggtctc ctacgcggac ttcgcggtgt ggcagcgccg ctggttcgcc
      961 ggtgacgagc tcacccggca gctctcctac tggaaggaac gcctcgaagg ggctccggcc
     1021 gtgctggagc ttccgaccga ccggccccgg cccccgatcc agagcttcga gggagccacg
     1081 gtcggcttca cccttccgaa agacctcagc gacgagatcg ccaagctggc gcgcgcccac
     1141 gaggtcacga ccttcatggt cacgctcgcc gccttcgagc tgttgctgca ccgctacacc
     1201 ggcgccaccg acatagtcgt cggctctccc atagcgggac gcaaccgctc cgagatcgag
     1261 ggcctcatcg gcttcttcgt caacaccctc gtgttgcgga ccgacctgtc gggcgacccc
     1321 accttcgcgg agctgctcga acgagtccgc gaggtcgccc tcggtgccta cgcccaccag
     1381 gaccttccct tcgagaagct cgtcgaggag ctcggcatcg accgtgacct cgcgcacagc
     1441 ccggcggtgc aggtgatgtt cgtgttccag aacacacctc cgggcgacct cgctctcgcc
     1501 ggaacgaaca gagcgcggct cgacgccccc acgaacgagg tcgccgtcga cgtgacgctc
     1561 gacgtcaccg agaccgaagc cgggctcgta gcgcaggccg tctacaacgc cgacctgttc
     1621 gaccggcgga cgatggaggc gttcctgcgc cacttcggca acgtcctgga ggccgtcgcg
     1681 cgcgatgcgg agacgccgct gtcgcgcgtc gagctgctcg acccggacga gcgggcgacg
     1741 atcgtcacgg gcttcaaccc cccgccgacc ccgttccccg acgacgtgcg cgtgcacgag
     1801 gtgttcgagc gcgtcgccga cgagcgcggg aacgcgccgg cggtcgtgta cggcgacgag
     1861 acgctgtcgt acacggagct ggacgtgaga gcgaaccggc tcgcccgcca cctcgcggcg
     1921 cacggcgtcg gccgcggtga cctcgtcgcg gtcgtgctgg agcgggggat cgacctcgtc
     1981 atctcgctgc tcgccatcct caaggccggg gctgcgtacc tgccgatcga tcccgagaac
     2041 ccgcgacgcc gcactgcgtt catgctcgag gactcggaag cccgcgtcgc cgtcacgggc
     2101 gggaccacgc cggccggcgg cctcgcggcc gccacggtga tctcgctcga cggcgacgcc
     2161 gcggcgatcg ccgctcacga cgcatcggcc cccgcggtgc ccggagaagc tgccgacgtc
     2221 gcctacgcga tctacacgtc gggctcgacc ggcacgccga agggcatctg catcccgcac
     2281 cgggcgatcg cgcggctcgt cctcgacacc gactacgtca agctcggccc ccactccgtc
     2341 gtcgcccagc tcgccaacgc gtcgttcgac gcggccacgt tcgagatctg ggggccgttg
     2401 ctcaacggag gcgtcgtggc cgggatcgag aaggacgtcg cgctggacgc aggccggctc
     2461 cgcgaggcgc tccgagcgca cggggtcgac accgcgttcc taaccaccgc gctgttcaac
     2521 cagctcgtgg ccgaggaccc gtcgttgttc tcgtcgctcg acacgctgct ggtcggggga
     2581 gaggcacacg acccgtcgat ggtccgccgt tgcctcgacg ccgagccgcc ggcgaagctg
     2641 tgcaacgtct acggcccgac cgagtgcacg acgttctcga cctggtaccc gatcgagagc
     2701 gtgccgaagg gcgcgacgtc gatccccatc gggcggccga tcgcgaacac gaaggtccac
     2761 gtgctcgact ccgccatgaa ccccctgccc gtcggcgtcc ccggagaggt ccacgtcggg
     2821 gggccggggc tcgcagccgg gtacctcgac cggccggagc tgacgaagga gcggttcgtg
     2881 cccgacccgt tcgcctcgga cgatccctcg gcgcgcctct acaagaccgg cgacctcggc
     2941 cgctacctgc cgtcgggcga cgtcgagttc ctcgcgcgga tggactccca ggtgaagatc
     3001 cgcgggttcc gcgtcgagct cggcgaggtc gaggcggctc tcgccgagca cccgcacgtc
     3061 aaggagtcgg tcgtcacggt tgccgaggcc cgaggcgaca agcggctcgt cgcgtacttc
     3121 gccggcgacg gcgtggaggt cgcggagctg cgcaggttcg tgtccgagcg tgtgcccttc
     3181 tacatggtgc cgtcggagta cgtggagctc gatgctctcc cgttgaacgc gaacggcaaa
     3241 gtcgaccggc gggcgctgcc cgcgccggac ccgtcccggc actccgccgg cgaagaacgc
     3301 gtcgcgccgc gcaacgagac cgagtcgaag ctggcctcga tctgggagga gctcctgtcg
     3361 aagcggccgg tcggcgtgca cgacgggttc ttcgagctcg ggggacactc tctcctcgcg
     3421 acccagctcg tgtcgcgcat cagggacgcg ttctatgtcg agctggagct gcgggcggtc
     3481 ttcgaggagc cgacgatcgc gcggcttgcc ccgcgggtcg acgccgcgcc gcgggcggac
     3541 tcgcccggga tccccgagct gaaagcgctg ccccggcatt cgagtcgtct cgacgggtcg
     3601 tcgaacagat gagcgcggac cgcaaaaggc tgctcgagct catgctgaag caacgcgggc
     3661 tcgccggcgc gctcacggac ggccccgtcc cgctcgggcc ggacgatgaa cggctgctct
     3721 ccttcgggca gcagaggctg tggttcttcg acgagtggca gccgaacggg tcggtctaca
     3781 acgtgcccgg cgcgatcgag ctgaccggac cactggacgt ggacgcgctg cagcacgcac
     3841 tcgacacgat ctcggcgcgg cacgactcgc tgcggatgac attccactcg gccgggccgg
     3901 agccgcggct ctcggtcgcg ccaccggccc ccgtcgacct tccgctgacc gacctcggcg
     3961 acgtgccgga ggccgagcgc tacgagcgcg cgatcgcgct ggcaaacgag atcgcgctcc
     4021 ggcccttcga cctgtccgcc ggccccctgt ggcgcaccgc gctgatccgc ctcgaagaag
     4081 accgccacgt cttcgtgacc tgcatgcacc aaatcgtgtc ggacggctgg agcctaggca
     4141 tcctcgcctc ggagctctcc gagctctact ccgcaaggat cgaaggtcgc gagcccgacc
     4201 tcccggaact agaggtctcc tacgccgact tcgcggtgtg gcagcgccgc tggttctcgg
     4261 gcgacgagct cacccgccag ctctcctact ggaaggaacg cctcgaaggg gccccggcgg
     4321 tgctggagct tccgaccgac agaccccgac ccccgatcca gagcttcgag ggagccacgg
     4381 tcggcttcac ccttccgaaa gagctgagcg acgagatcgc caagctggcg cgcgcccacg
     4441 aggtcacgac cttcatggtc acgctcgccg ccttcgagct gttgctgcac cgctacaccg
     4501 gcgccaccga catagtcgtc ggctctccca tcgcgggacg caaccgctcc gagatcgagg
     4561 gcctcatcgg cttcttcgtc aacacgctcg tgctgcgcac cgacctgtcc ggcgacccca
     4621 ccttcgcgga gctgctcgaa cgcgtccgcg aggtcgccct cggcgcttac gcccaccagg
     4681 acctcccctt cgagaagttg gtggaagagc tgcggcccgc gcgcgacctg agcatctcgc
     4741 cggtgttcca ggtgtcgttc gcgctgcagg acgtgcgcgc ggctgacttc gagctgacgg
     4801 ggctcgaggt ggggttcacc cgggtgaagg acgagagtgc caaggtcgac ctctcgctgt
     4861 tcctgttcga aggcgacgaa gggctgttcg gcgagttgaa ctactccacc gcgctgttcg
     4921 aggagaccac gatccgttcg atgatcgccc acttcgaagc ggtcctcgcg gcggtcaccg
     4981 ccgaaccggc cgtccccgtg tcccgccacg cgttgatctc ggacgaggag gggagagacc
     5041 tcgtcgagga gctcaacccg ccgctcccgg ctgcgccggc cggccccctc gtccacgaac
     5101 gtctcgccga gcttgcccgg tcccacccgg acgacgtcgc cctcgtcgca ggcaacgcgt
     5161 cgcttacgtt cggcgagctc gaccggcgcg ccgaggccgt tgccgcgtca ctggatgcag
     5221 gcccgggcga cgtcgtcgcc gtcctgatgg agcggtccct cgaacaggtc gtggctcagc
     5281 tggggatcct gaaggcgggc gcggcctatc tgccgctcga tcccgcgtat ccgccgaaac
     5341 ggctgtcgct catgatcgag gacgcgcggc cgaaggccgt gatctcctcg ctcgaggacc
     5401 tgccttcgcc ggggccgcgc cgcgacgcgg ctgtcaacgg cgaagacctc gcgtacgtca
     5461 tctacacgtc gggttcgacc gggacgccga agggcgtcgg ggtcacccac gctaacctcg
     5521 ccaacctcct ggagtgggac cgcggtcagt acctcggtcc cgggcgcacg acgtcgctcg
     5581 ccccgttgtc ggccgacgcg tcggtctggg agctgtggcc tccgctcgcc gcggggatgc
     5641 cggtggtgct gatcgaccgg gagacggccc gcgatccggc cgcgctcgtg cgtgccttcg
     5701 aacgtcaccg agtcaccaac gggttcgtgc ccacggcgac cgcgcacgtg ctgctgcggg
     5761 ccgaggcgcc cggtgggttg gagacgctga tgaccgccgg cgagcggctc acggcggccc
     5821 ccgacgagac cttcgccgct acggtcgtga acctgtacgg gccgacggag gccacggtcg
     5881 ccgcgaccgc gggcatcgtc gcccccggag gcggcatccc cgacatcggc acccccgtca
     5941 acggcgtgcg cgtctacgtg ctggacgaac acctccgtcc cgtgccgcgc gggctccccg
     6001 gagaggtcta catcggaggc cgtggcgtcg cgagccacta cctgaacgcc cctgggctca
     6061 ccgctcgggc cttcgtgccc gaccccttct cgtccgagcc gggagcgcgc atgtaccgct
     6121 cgggcgaccg ggcccgccac cgccccgacg ggacgatcga cttcctcggc cgcggagacg
     6181 agcaggtgaa ggtgcgcggg taccgggtgg agcctgccga ggtcgaggcc gcgctgcgga
     6241 cgcatcccgc ggtctccgag tgcgcggtgg ggaccgtggt cgacaagtcc ggcgcgacca
     6301 gcctcgtcgc gtacttcgtc gccgcgcccg agccgcccgt gtcggagttg cgctattacc
     6361 tctccgacct cctgcccgcg cacatggtgc cgtcgtcgtt cgtgacgctg gaggcgctcc
     6421 cgctgacgcc cgccgggaag gtcgaccgtg ccgccctgcc cgcgccgggg gccgagcgac
     6481 ccgacgtcgg ccacgcctac gaggagccgc ggacagagac cgagaaggtc ctggcggaga
     6541 tctgggccga cgtcctcgga gtcgaacgag tgggccgtac cgacaacttc ttcgacctcg
     6601 gcgggcattc gctgctcggg acccagctga tgtcccgcat ccgcaaccgg ttcgaggcag
     6661 aggttccgct gcgcgcgctg ttcgagcggc cgaccgtcgg cggcctcgct gcgacactcg
     6721 acacgacctc gcgcaccgag tcgctgccgc tggtcgcggc cgagcgcggc gacctcccgg
     6781 tcctctcgtt cgcgcagcag cggatgtggt tcatcgacca gatcgacccc gcgagctcga
     6841 cctacaacgt ccctgtcgcg ctgcggctca ccgggcgcgt cgacggcgca cgcctcgaac
     6901 gagcgctgaa cgcgatcgtc gagcgtcacg agaccctgcg cacgacgttc acgaccgtgc
     6961 gcggcgagcc ccgtcaggtg gtagcggacg agggccgcgt gtccctgtcc gtcgtcgacc
     7021 tcgcctacct gggcgacgac gagcgcgagg aggcggcgat gaaggtcgtc gcggaggagg
     7081 ccgaccgccc cttcgacctc gctgcgggcc ccttgtggcg gacgacgctg atcgagctcg
     7141 gcgacgagga ccacctgctc gtgatctgca tgcatcacat cgtgtccgac gggtggagcc
     7201 tcggggtctt cgcgtcggag ctgagcgaga tctacacggc cttcgtggac gaccgcgagc
     7261 ccaagctcga gccgctcccg ctgcagtacg ccgacttcgc catctggcag cgcgagtggt
     7321 tccagggcga ggagctcgac cgtcagatga cgtattggaa gcgtcatctc gaaggcgcgc
     7381 cggcggcgct cgacctcccg accgactacc cgcggccccc gatccagacg ttcgagggcg
     7441 cgtcgatcaa cttcgcgctg ccggccggcc tgtccgccga cgtgaaggag ctcgcccggc
     7501 gcaacggcgt gacgatgttc atgctcgggc tcgccgcgtt cacgaccttg ctccatcgct
     7561 acaccggtgc ggccgaggtc gtcgtgggca cgccgatcgc cggccgcaac cggtccgaga
     7621 tcgagggcct gatcggcttc ttcgtgaaca cgctcgtgat gcgcaccgac ctctcgggcg
     7681 acccgacgtt cagcgagctg acggagcgcg tgcgcgacgt cgcgctcggc gcctacgcgc
     7741 accaggacct cccgttcgag aagctcgtcg aggagatgaa gccgccgcgc gacctcagcc
     7801 actcgccgct gttccaggtc gcgttcggct accagaacac accggcgccg gagttcgagc
     7861 tgacggggct ggggatcgac cttcccgagc tcgacgacac cagcgcgaag ttcgacctca
     7921 cgctcgacat gggagagacc gaggaggggc tcggcggatc gctcaactac gcgacggctc
     7981 tcttcgagcg cacgacgatg gaggagatgc tcgcccactt ggagacgctg cttcgtgcgg
     8041 cggtggcgga ccccgagacg aggctgtcgg agcttccgat cctgaccccg gaggagcggg
     8101 acgtcgtcgt ccaccggttc aacgacacgg gggccgagca cgacgcggac aagctgatgc
     8161 acgagttctt cgaggagcac gcgcgctctc gcccggatgc cgtcgcggtc cgcttcgagg
     8221 gcggcgactt cagctacggc gagctgaacg agcgggccaa ccgcctcgcg caccacttgc
     8281 ggagcctcgg cttgcgtccg gggagccg
//
LOCUS       DASQDO010000153        10405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_382821, whole genome shotgun sequence.
ACCESSION   DASQDO010000153 DASQDO010000000
VERSION     DASQDO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382821"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VEV43_04495"
     CDS             complement(<1..396)
                     /locus_tag="VEV43_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYP22810.1"
                     /translation="MDRDDRERRAAGSIGELDERLRGWAGETVEGLTPGFDAPAAELS
                     DAVSLYLLDVVPKPPARGDRRPPLQAEVRYLVTAWAGDPATAHAALGDLFFGALQHPD
                     LEVEPLPVPVPTWSAFGVPPRPSFVIKAVV"
     gene            complement(442..966)
                     /locus_tag="VEV43_04500"
     CDS             complement(442..966)
                     /locus_tag="VEV43_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22811.1"
                     /translation="MLKKIEIGMTVVSIMLGTYEILGRTGVVSGRTPIEAVQDAVAGE
                     SGAAPTDNGTERSAPQAQDLVLRVPSCQVTADTVTLEVVAASAAPTRTLEGVQVSAAS
                     GALTETTTVTFEDTTTSSTSSVSLNTGPGSHTITLVVDADDRYAETDESNNVVVATAE
                     VPADPAVTGSCAPV"
     gene            complement(1029..1475)
                     /locus_tag="VEV43_04505"
     CDS             complement(1029..1475)
                     /locus_tag="VEV43_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HYP22812.1"
                     /translation="MVRLSLEELYKEVILDHYRAPRNKGRIDPHDVALERNNPLCGDE
                     IELFLKFDGDTVTGVAFEGKGCSISQASTSMMTEKVKGLAAKDAAQLAESIKRMMAGE
                     EDGDADALGDLVSLKGVVKYPVRIKCALLGWNTLLEGLAEGGKAEA"
     gene            complement(1463..2686)
                     /locus_tag="VEV43_04510"
     CDS             complement(1463..2686)
                     /locus_tag="VEV43_04510"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HYP22813.1"
                     /translation="MSLDAEVVRKDFPIFETLAHGKPLVYLDSAATSHKPRAVIDRIV
                     RFYETENANVHRGIYELGEKATAAYEGARAASARFVGARSTRSIVFTRGTTEAINLVR
                     YTWARENVREGDEILVTDMEHHSNLIPWQLHCLDTGAVLRHLPIDDEGRLRVDLLGDY
                     LTDKTKLVAINLMSNVLGTINPVRQIADAAHSVGARVLVDAAQAAPHLSFDVNELDAD
                     FLAFSAHKMCGPTGAGVLYGREDLLEAMPPFHGGGEMIREVWSDRATFAEVPYKFEAG
                     TPNVAQVVGMGAAIEYLEDLGMDAVREHEIEMTEYALGRLRETGATIYGPPDAHDRGG
                     VVAFNVPEVHPHDMATIVDQEGVCIRAGHHCAQPLMRCLGVPATARASFYVYNTRDDV
                     DRLIDALDKAKGWFG"
     gene            complement(2697..3500)
                     /gene="sufC"
                     /locus_tag="VEV43_04515"
     CDS             complement(2697..3500)
                     /gene="sufC"
                     /locus_tag="VEV43_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HYP22814.1"
                     /translation="MAERLLHIEDLHVEVEGREILKGLNLSLDTGDIHAIMGPNGSGK
                     STLAYVLTGRPGYEVTAGKVLYKGEDVLDLEPDERAHRGIFLAFQYPTEVPGVSVVNF
                     LRTAYNAINPDAQKSAMQFRMYLQEKIDLLEIPSELVDRYVNQGFSGGEKKRNEILQM
                     AVLQPELAIMDETDSGLDIDALKHVSAGVNKLAGPDVGILLITHYQRLLNYIRPSTVH
                     VLMGGRIVKSGGFDLAERLEAEGYAGIAREAGLTAEELAAVEERERAKV"
     gene            complement(3509..3835)
                     /locus_tag="VEV43_04520"
     CDS             complement(3509..3835)
                     /locus_tag="VEV43_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HYP22815.1"
                     /translation="MTFRPVARLDEIGDGEMRQFSVGGELVGVYRVGDDVYALSDVCS
                     HQEAYLTEGDFEPEDLEVECPLHGSRFNVATGAVRILPATKPVASYETKVEGGEILVG
                     PKKTGE"
     gene            complement(3836..5059)
                     /gene="sufD"
                     /locus_tag="VEV43_04525"
     CDS             complement(3836..5059)
                     /gene="sufD"
                     /locus_tag="VEV43_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HYP22816.1"
                     /translation="MTPRLDRDALEALVAATGEPGWLAERRIEAFDLFEKLDMPDPKK
                     EEWRYTDVRGFDFDLYAAPEPAKRSLDLPKELAGKGVVFTDLVNADAELVKEHFFTEV
                     PVDEHRFTALHGAFHSDDVIVHVPRGVEVDMPLEVLRDVDKGGSAFPHTTIVVEDQAS
                     LTFVDRFKSEDSLMPSMCSSVVEVTAGRGATVNYISLQEWGRGVSHFQTQRFTGYRDS
                     TVRSLAVNLGSKFARTQVESVLKGEGSFSEMLGLYFADAEQHFAQRTLQQHVAAHATS
                     DLLYKGALKERSRSEYSGLIKVGKGAQGTDAYQANRNLVLSEDALAHSIPQLEIEANE
                     VRCTHGATVSPVEEEHLFYLMSRGIDRVTAQKLVVFGFFGEVLDRIRVPQVRDDLSDA
                     IAAKVEGTQRLEVAV"
     gene            5146..6069
                     /locus_tag="VEV43_04530"
     CDS             5146..6069
                     /locus_tag="VEV43_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYP22817.1"
                     /translation="MHEQPERSNDSGAGLGLLAVIGAAACWGIAAAVAKDLFEAGVEP
                     IELAQARSYIAAVGFSLLPGAWSGGIGVTRQSAPALIGLGLSIPAVNGVYYLAIQRLA
                     VAVALVLEYSAPVLVVAWTAVRTRKFPRPLLLVTLGAAIVGVVFVAELPAGNVGSLDG
                     IGLLLGLAAAVFFSAYTLLNEATGSHFGPIPAMARAFAIAALFWLVYQIPRGFPEDLV
                     HPDNILRVLLIGVGGTLVPFLLFIWGVAKVKSEKAAIAATIEPPLAGVIAWLWLGERL
                     SAMQVGGGVLILAAVVALQLERKQPVLAPEP"
     gene            complement(6066..7046)
                     /locus_tag="VEV43_04535"
     CDS             complement(6066..7046)
                     /locus_tag="VEV43_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP22818.1"
                     /translation="MRVAIVGAGVMGAATARSLARSGHDVTVFEQFGFAHTRGSSHGA
                     TRVFRFSYPVVEYVEMAMEALELWREAERDTGEDLLRTTGGLDAGKALDDHVAALQEC
                     GAECEVLDRAEAAARFPWLTPGDGPLLFQGDGGIAFADRAVEAFLRSASEHGATLRPE
                     TKVASIEDLDSDVVVVTAGGWVKELVEPLGIEVPARPTRETVAYFEFDEPRLTTLVEW
                     GDPSIYCLPSGPGVLKAGEHQAGATTDPDEEGRVDEASVARLSEWVAKRFPGANPKPI
                     HAETCIYTNTPDDRFVLERHGDVVIGSPCSGHGFKFAPLIGERLAELAAG"
     gene            7072..8244
                     /locus_tag="VEV43_04540"
     CDS             7072..8244
                     /locus_tag="VEV43_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP22819.1"
                     /translation="MKRALLSLSLLVALPAPHAAAAPTCFGKKATITDGKREVVRGTP
                     GDDVIVTRGIIRLVRAGAGDDLVCARGYNDTIYGGKGDDRVRLVRDDDGSYLYGGDGN
                     DDLGGPGAFSYGGRGDDVIAGETLAGGPGDDDITAAEYAVLWYSGAPRAVHVDLKRGI
                     ATGGAGRDRLHGEFARVVGSKRTDVLRGDDHSDWLSGGGGDDLLVGRGGDDLLEGDEG
                     IGGRPGGDLIRGGDGDDEVFGGPGKDDLYGGPGRDEADPGPGDDAVWLGPGTAFVSGD
                     HEGDDALLAGGGRDYLVFSLWGGDVQVDLAAGTVEGAGSDVVRGFEHVFGGPGDDVIR
                     GDEWVNRLRGNDGNDTLDGAGGGDVLRGGRDVDHADGGDGTDECEAETTLACEDAP"
     gene            complement(8241..8756)
                     /locus_tag="VEV43_04545"
     CDS             complement(8241..8756)
                     /locus_tag="VEV43_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP22820.1"
                     /translation="MVDALPIETERLLIRSSVVEDAEAFLDIYSDPEVTRWLGGPISK
                     TVEQVRERIGLRRSIQQRHGFSLWTVVEVETGTIIGSAGLFPVEGDGPDVEVAYHFRK
                     DRWGRGYATEAAKACLDYGFDVAGLDTIIGLVAPENIASARVLEKCGMTRAGTAHHYD
                     MDLTKYTAARR"
     gene            complement(8859..10292)
                     /gene="sufB"
                     /locus_tag="VEV43_04550"
     CDS             complement(8859..10292)
                     /gene="sufB"
                     /locus_tag="VEV43_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HYP22821.1"
                     /translation="MATEAEDLKVQQENEQVRALKGEYKYGWHDATSYDVAPTKGINH
                     QVLDMMSDRKNEPDWMRKFRHKALDVFYRKPMPGWGADLSGIDFDDIYYYIKPMNQVK
                     SWDDMPEDIKSTWDKLGIPEAEKKYLGGVTAQYESEVVYHKTQEHLERQGILFSDMDT
                     AVREHPDLVKQYFGTIIPPADNKFAALNSAVWSGGSFIYVPPGVEVEIPLQAYFRINQ
                     ENMGQFERTLIIVDENAYVHYVEGCSAPIYSSDSLHSAVVEIVVKDHGRCRYTTIQNW
                     SNNVYNLVTKRAAAHKGAIMEWVDGNLGSKVTMKYPAVYLLGEHAKGEVLSIAYAGDG
                     QHQDAGAKMIHAAPDTQSAITSKSISKDGGRGGYRGLVRIEEGAERAKSHVICDALIL
                     DDHSRSDTYPYIEIEEETAAVSHEATVSKVSDEQLFYLMSRGLSETEAMQMIVRGFIE
                     PITKELPMEYAVELQRLVELQMEGSVG"
BASE COUNT         1438 a         3553 c         3665 g         1749 t
ORIGIN      
        1 cacgacggct ttgatcacga acgacggacg cgggggcacg ccgaacgcgg accacgtcgg
       61 gaccggcacc ggtaggggct cgacctcgag gtccgggtgc tggagggcgc cgaagaacag
      121 gtcgccgagc gcggcgtgcg ccgtcgcggg atcgccggcc cacgccgtca cgaggtagcg
      181 cacctcggcc tggaggggtg ggcgcctgtc gccgcgcgcc ggcggcttcg gcacgacgtc
      241 gaggaggtag aggctgacgg cgtcggagag ctccgccgcc ggcgcgtcga acccgggggt
      301 gagcccctct acggtctccc cggcccagcc gcgcagacgt tcgtcgagct caccgatcga
      361 gccggccgca cgccgttccc tgtcgtcgcg atccacgacg ccatttcaac caccggcgag
      421 tggagcgcgc tgttaggtcg cttacacggg cgcgcaggat ccggtcacgg ccgggtccgc
      481 cgggacctcg gccgtcgcga ccacgacgtt gttgctctcg tcggtctcgg cgtaacggtc
      541 gtccgcgtcg acgacgagcg tgatcgtgtg cgagccgggc ccggtgttca gcgacaccga
      601 cgacgtcgag ctggtcgtcg tgtcctcgaa ggtcacggtc gtcgtctcgg tgagcgcgcc
      661 ggaggccgcg gagacctgga cgccttcgag cgtcctcgtg ggcgccgcgc tggcggcgac
      721 gacttcgagg gtgaccgtgt ccgccgtgac ctgacagctg gggacgcgca ggacgaggtc
      781 ctgtgcctgg ggagcggacc gctcggtccc gttgtccgtc ggggcggccc ccgattcccc
      841 ggcgaccgcg tcctgcacgg cttcgatggg ggtgcgaccg gagacgacgc cggtgcggcc
      901 gaggatctcg tacgtgccca gcatgatcga cacgacggtc atcccgatct cgatcttctt
      961 gagcactcgc cagctcctgc cgcgccgccc ggacggtcga gcaccttagc tgatcgagca
     1021 agcgcgggct aggcctcggc cttgccgcct tccgccaggc cctcgagcag cgtgttccag
     1081 ccgagcagcg cgcacttgat gcgcaccggg tacttcacga cgcccttcag cgagaccagg
     1141 tcgccgagcg cgtccgcgtc gccgtcctct tcgcccgcca tcatgcgctt gatcgactcg
     1201 gccagctggg ccgcgtcctt cgccgcgagg cccttgacct tctcggtcat catcgacgtg
     1261 ctggcctggc tgatcgaaca gcccttgccc tcgaacgcga cgccggtgac ggtgtcgccg
     1321 tcgaacttca ggaacagctc gatctcgtcg ccgcacagcg ggttgttgcg ctcgagcgcg
     1381 acgtcgtgcg ggtcgatgcg gcccttgttc cggggcgcgc ggtagtgatc gaggatgacc
     1441 tctttgtaaa gctcctccag gctcagccga accacccctt cgccttgtcc agcgcgtcga
     1501 tcagccggtc gacgtcgtcg cgcgtgttgt agacgtagaa cgaggcccgg gcggtcgcgg
     1561 gaacgccgag gcagcgcatc agcggctgcg cgcagtggtg ccccgcgcgg atgcagacgc
     1621 cctcctggtc gacgatcgtc gccatgtcgt gcgggtgcac ctccgggacg ttgaacgcga
     1681 cgacgccgcc gcggtcgtgc gcgtcgggag gcccgtagat cgtggccccc gtctcgcgca
     1741 gccgtccgag cgcgtactcg gtcatctcga tctcgtgctc gcgcaccgcg tccatcccga
     1801 ggtcctcgag gtactcgatc gcggccccca tgccgacgac ctgcgcgacg ttcggcgtgc
     1861 cggcctcgaa cttgtagggg acctcggcga acgtcgcccg gtcgctccac acctcgcgga
     1921 tcatctcgcc gccgccgtgg aacgggggca tcgcctcgag caggtcctcg cgcccgtaca
     1981 gcacgccggc ccccgtcggc ccgcacatct tgtgagcgga gaacgccagg aagtcggcgt
     2041 cgagctcgtt cacgtcgaac gacaggtgcg gcgcggcctg tgccgcgtcc acgagcacgc
     2101 gggcgcccac ggaatgcgcg gcgtccgcga tctgccgtac ggggttgatc gtcccgagca
     2161 cgttgctcat gaggttgatc gctacgagct tcgtcttgtc cgtcaggtag tcgccgagga
     2221 ggtcgacgcg cagccgcccc tcgtcgtcga tcggcaggtg gcgcagcacc gcccccgtgt
     2281 cgagacagtg gagctgccac gggatcaggt tcgagtggtg ctccatgtcg gtgacgagga
     2341 tctcgtcgcc ctcgcggacg ttctcccgcg cccacgtgta gcggacgagg ttgatcgcct
     2401 cggtcgtccc gcgcgtgaac acgatcgagc gcgtgctgcg ggccccgacg aagcgggcgc
     2461 tcgcggcgcg ggccccctcg tacgcggcgg tcgccttctc gccgagctcg tagatccccc
     2521 ggtggacgtt cgcgttctcg gtctcgtaga agcgcacgat gcggtcgatc accgcccgcg
     2581 gcttgtgcga cgtcgcggcg gagtccagat acacgagcgg cttgccgtgg gcgagcgtct
     2641 cgaatatcgg gaagtccttg cggacgacct cggcgtctag cgacatcgca gcaccatcag
     2701 accttcgccc gctccctctc ctcgaccgcg gcgagctcct cggccgtcag cccggcctcg
     2761 cgcgcgatgc cagcgtatcc ctcggcctcg agacgctcgg cgaggtcgaa cccgcccgac
     2821 ttcacgatgc ggccgcccat gaggacgtgg acggtcgacg gccggatgta gttcagcagg
     2881 cgctggtagt gcgtgatgag aaggatgccg acgtcggggc cggccagctt gttcacgccg
     2941 gcggagacgt gcttgagcgc gtcgatgtcg aggccggagt cggtctcgtc catgatcgcg
     3001 agctcgggct ggagcaccgc catctgcagg atctcgttgc gcttcttctc gccgccggag
     3061 aagccctggt tgacgtagcg gtcgacgagc tcgctcggga tctcgagcag atcgatcttc
     3121 tcctgcaggt acatgcggaa ctgcatcgcg ctcttctgcg cgtcggggtt gatcgcgttg
     3181 tacgcggtcc gcaggaagtt cacgaccgag acgcccggca cctcggtcgg gtactggaac
     3241 gcgaggaaga tgccgcggtg cgcgcgctcg tcgggctcga ggtcgaggac gtcctcgccc
     3301 ttgtagagca ccttgccggc ggtgacctcg tagccgggcc gcccggtcag gacgtacgcg
     3361 agcgtcgact tgcccgaccc gttcgggccc atgatggcgt ggatgtcgcc cgtgtcgagg
     3421 ctgaggttga ggcccttgag gatctcgcgg ccctcgacct cgacgtgcag gtcctcgatg
     3481 tgaagaagtc tctctgccat gtgctgcgtc actcccctgt cttcttgggc ccgacgagga
     3541 tctcgccgcc ctcgaccttg gtctcgtagc tcgccaccgg cttcgtcgcc ggcaggatcc
     3601 gcaccgcgcc cgtcgcgacg ttgaagcgcg acccgtgcag cgggcattcc acctcgaggt
     3661 cctcgggctc gaagtcgccc tcggtgaggt acgcctcctg gtgcgagcag acgtcgctca
     3721 gcgcgtacac gtcgtcgccg acgcggtaga cgccgacgag ctcgccgccg accgagaact
     3781 ggcgcatctc gccgtcgccg atctcgtcga ggcgcgcgac cggacggaac gtcatctaga
     3841 ccgccacctc gagccgctgc gtcccctcga ccttcgccgc gatcgcgtcg ctcaggtcgt
     3901 cgcgcacctg cgggacccgg atgcggtcga ggacctcgcc gaagaaaccg aacacgacga
     3961 gcttctgcgc cgtcacgcgg tcgatcccgc ggctcatcag gtagaagagg tgctcctcct
     4021 cgaccgggga cacggtcgct ccgtgcgtgc accggacctc gttcgcctcg atctcgagct
     4081 gcgggatcga gtgcgcgagg gcgtcctcgg acagcacgag gttgcggttc gcctggtacg
     4141 cgtcggtgcc ctgggccccc ttgccgacct tgatgagccc ggagtactcg ctgcgcgacc
     4201 gctccttcag cgcccccttg tagaggaggt ccgacgtcgc gtgcgcggcc acgtgctgct
     4261 gaagggtccg ctgcgcgaag tgctgctcgg cgtcggcgaa gtagaggccg agcatctccg
     4321 agaacgagcc ttcgcccttg agcacggact cgacctgcgt gcgcgcgaac ttcgagccga
     4381 ggttgaccgc gaggctgcgc acggtcgagt cgcggtagcc ggtgaaacgc tgggtctgga
     4441 agtgcgagac gccgcggccc cactcctgca acgagatgta gttgacggtg gcgccgcggc
     4501 ccgccgtgac ctcgacgacg ctggagcaca tcgagggcat gaggctgtcc tcggacttga
     4561 accggtcgac gaacgtcagc gacgcctggt cctcgaccac gatcgtcgtg tgcgggaacg
     4621 ccgacccacc cttgtcgacg tcacgaagga cctcgagcgg catgtcgact tcgacgccgc
     4681 gcggcacgtg gacgatgacg tcgtcggagt ggaacgcgcc gtggagcgcg gtgaagcgat
     4741 gctcgtcgac ggggacctcg gtgaagaagt gctccttgac cagctcggcg tcggcgttga
     4801 cgaggtcggt gaagaccacg cccttgccgg cgagctcctt cgggaggtcg agcgagcgct
     4861 tcgccggctc gggggccgcg tagaggtcga agtcgaagcc ccggacgtcg gtgtagcgcc
     4921 actcctcctt cttcgggtcg ggcatgtcga gcttctcgaa gaggtcgaag gcctcgatcc
     4981 ggcgctcggc cagccagccg ggctcgccgg tggcggcgac gagggcctcg agggcgtctc
     5041 ggtccagtct cggcgtcatc tgagtcggtt cagctcccag ggtcaggggt cgtcggttag
     5101 gcaagcctaa cctcgcgggt ccacaggacg tttaccctgc tcaccatgca cgaacagccc
     5161 gagcggtcga atgattccgg ggccgggctc ggtctgctcg ccgtcatcgg ggccgccgcc
     5221 tgctggggca tcgctgcggc ggtcgcgaag gacctgttcg aggccggggt cgagccgata
     5281 gagctggccc aggcccgctc ctacatagcc gccgtcgggt tctccttact gccgggcgcg
     5341 tggtccggtg ggatcggcgt gactcgtcag tcggcccccg ccctcatcgg gctcggcctc
     5401 tcgatccccg ccgtcaacgg cgtctactac ctcgcgatcc agaggctcgc ggtcgccgtc
     5461 gcgctcgtgc tcgagtactc ggcccccgtg ctcgtggtcg cgtggaccgc ggtccgcacg
     5521 cgcaagttcc cgcggccgtt gctgctggtg acgctcggtg cggcgatcgt cggagtcgtg
     5581 ttcgtcgccg agctccccgc cggcaacgtc gggagcctgg acggcatcgg gctgctcctc
     5641 gggctcgcgg ccgcggtgtt cttctccgcc tacacgttgc tgaacgaggc gaccggctcc
     5701 cacttcgggc cgatcccggc gatggcgcgc gcgttcgcga tcgcggccct cttctggctc
     5761 gtgtaccaga tcccccgcgg cttccccgaa gacctcgtgc accccgacaa catcctgcgc
     5821 gtcctgctca tcggcgtcgg cgggacgctc gtgccgttcc tgttgttcat ctggggagtg
     5881 gcgaaggtga agtccgagaa ggccgcgatc gcggcgacga tcgagccccc gctggccggc
     5941 gtgatcgcgt ggctgtggct gggcgagagg ctctcggcga tgcaggtagg cggcggagtg
     6001 ctgatcctcg cggcggtggt agcgctacag ctggaacgaa agcagccggt cctcgcgccg
     6061 gagccctagc cggcagcgag ctccgcgagg cgctcgccta tcagcggcgc gaacttgaag
     6121 ccgtggccgc tgcacggcga gccgatcacc acgtcaccgt gccgttcgag cacgaaccgg
     6181 tcgtccggcg tgttcgtgta gatgcacgtc tccgcgtgga tgggcttcgg gttcgccccg
     6241 gggaaccgct tcgccaccca ttcgctcagc cgcgccaccg acgcttcgtc gacgcggccc
     6301 tcttcgtcgg ggtccgtggt ggcgccggcc tggtgctcgc cggccttcag cacgcccggc
     6361 ccgctcggca gacagtagat cgacgggtct ccccactcga cgagggtcgt gagccgcggc
     6421 tcgtcgaact cgaagtaggc gacggtctca cgcgtcggcc gggccgggac ttcgatcccg
     6481 agcggctcga cgagctcctt cacccagcct cccgccgtga ccacgaccac gtcgctgtcc
     6541 aggtcctcga tcgacgccac cttggtctcg gggcgcagcg tcgccccgtg ctcgctcgcg
     6601 ctgcgaagga acgcctccac cgcgcggtcc gcgaacgcga tgccaccgtc gccctggaag
     6661 agcagcggcc cgtcgcccgg cgtgagccac gggaaacgcg ccgcggcctc cgcgcgatcg
     6721 agcacctcgc actccgcgcc gcactcctgt aacgcggcga cgtggtcgtc gagggccttg
     6781 cccgcgtcga gccctcccgt cgtccgcagc aggtcctcgc ccgtgtcgcg ctcggcctcg
     6841 cgccacagct cgagggcctc catcgccatc tcgacgtact cgacgacggg ataggagaaa
     6901 cggaacacgc gcgtggcgcc gtggctagag ccccgcgtgt gggcgaagcc gaactgttcg
     6961 aataccgtca cgtcgtggcc cgaacgggca agtgagcgcg cggtggccgc gcccatgact
     7021 ccggcaccta cgatcgctac gcgcatcgcc cgattctagg gagggacgta cgtgaagcgg
     7081 gcactcctgt cgctctcgtt gctcgtcgcg ctgcccgcgc ctcacgcggc ggcggccccg
     7141 acgtgcttcg ggaagaaggc gacgatcacc gacggcaagc gcgaggtcgt gcgcgggacg
     7201 ccgggcgacg acgtgatcgt gacgcgcggg atcatcaggc ttgtcagggc cggggcgggc
     7261 gacgacctcg tatgcgcgcg gggttacaac gacacgatct acggcggcaa gggcgacgac
     7321 cgcgtcaggc tcgtccgcga cgacgacggc tcctacctct acggcggtga cgggaacgac
     7381 gacctcggcg gcccgggcgc gttctcgtac ggcgggcgcg gcgacgacgt catcgcgggc
     7441 gagacgctcg ccggtggccc cggcgacgac gacatcacgg cggccgaata cgccgtgctc
     7501 tggtactcgg gcgcgccgcg cgccgtgcac gtagacctga aacgcgggat cgctacaggc
     7561 ggggccggac gcgaccggtt gcacggcgag ttcgcccgcg tcgtcgggtc gaagcgcacc
     7621 gacgtcctga ggggcgacga ccactcggat tggctgtcgg gtggaggggg tgacgacctg
     7681 ctcgtcggcc gcggcggaga cgacctgctg gagggggacg aggggatcgg cgggcgtccg
     7741 ggcggggacc tgatccgggg cggcgacggc gacgacgagg tgttcggcgg gccgggcaag
     7801 gacgacctgt acggcggacc ggggcgcgac gaggccgacc ccggcccggg agacgacgcc
     7861 gtctggctcg gccccggcac cgccttcgtg agcggcgacc acgaggggga cgacgccctc
     7921 ttggccgggg gcggtcgcga ctacctcgtc ttttcgttgt ggggcggtga cgtgcaggtg
     7981 gacctcgccg cgggaacggt cgagggggcc ggcagcgacg tcgtgcgcgg gttcgagcac
     8041 gtcttcgggg gccccggtga cgacgtcatc cggggcgacg agtgggtcaa caggctgcgc
     8101 ggcaacgacg ggaacgacac gctcgacggg gccggtggcg gcgatgtgct gcgcgggggc
     8161 cgggacgtcg accacgccga cggaggagac ggcacggacg agtgcgaggc cgagacgacg
     8221 ctcgcgtgcg aggacgcgcc ctagcggcgc gcggccgtgt acttcgtcag gtccatgtcg
     8281 tagtggtgcg ccgtgccggc gcgcgtcatc ccgcacttct cgagcacccg ggccgacgcg
     8341 atgttctccg gcgcgacgag gccgatgatc gtgtcgagcc ccgcgacgtc gaagccgtag
     8401 tcgagacacg ccttggcggc ttcggtcgcg tagcccctcc cccaccggtc cttgcggaag
     8461 tggtaggcca cctcgacgtc ggggccgtcg ccttcgaccg ggaagagccc ggcgctgccg
     8521 atgatcgtgc cggtctcgac ctccacgacg gtccacagcg agaagccgtg ccgttgttgg
     8581 atcgagcgcc tcagcccgat gcgctcgcgc acctgctcga cggttttcga gatcggcccg
     8641 cctagccatc tcgtgacctc ggggtccgag tagatgtcca ggaacgcctc ggcgtcctcc
     8701 acgacggagg aacggatcag caggcgttcg gtctcgatcg gtagcgcgtc taccaaggct
     8761 tgaccgcgac gggcttgggc gtcgcgttgc gggggccgag ccatagcgcg aagacgatcg
     8821 cgaacgcggc cagcttcatg cgccgcccgg agaccgcgtt aaccgacgga gccctccatc
     8881 tggagctcga cgagcctctg cagctcgacc gcgtactcca tcggcagctc cttcgtgatc
     8941 ggctcgatga agccgcgcac gatcatctgc atcgcctcgg tctccgagag gccgcgggac
     9001 atcaggtaga agagctgctc gtcggagacc ttgctgaccg tcgcctcgtg gctgaccgcc
     9061 gcggtctcct cttcgatctc gatgtacggg tacgtgtcgg agcgcgagtg gtcgtcgagg
     9121 atcagcgcgt cgcagatcac gtggctcttg gcgcgctcgg ccccctcctc gatccggacg
     9181 agcccgcggt agccgccccg gcctccgtcc ttgctgatcg acttggacgt gatggcgctc
     9241 tgcgtgtcgg gggccgcgtg gatcatcttg gcgcccgcgt cctggtgctg gccgtcgccc
     9301 gcgtaggcga tcgagaggac ctcacccttg gcgtgttcgc cgaggaggta gaccgccggg
     9361 tacttcatcg tgaccttcga gcccaggttg ccgtcgaccc actccatgat ggcgcccttg
     9421 tgcgcggcgg ctcgcttggt cacgaggttg tagacgttgt tcgaccagtt ctggatcgtc
     9481 gtgtagcggc agcggccgtg gtccttgacg acgatctcga cgaccgcgga gtgcagcgag
     9541 tcgctcgagt agatgggggc cgagcagccc tcgacgtagt gcacgtacgc gttctcgtcg
     9601 acgatgatca gcgtccgctc gaactggccc atgttctcct ggttgatgcg gaagtaggcc
     9661 tggagcggga tctcgacctc gacgcccggc gggacgtaga tgaacgaccc gcccgaccag
     9721 accgcggagt tgagggccgc gaacttgttg tcggccggcg ggatgatcgt gccgaagtac
     9781 tgcttgacga ggtccgggtg ctcgcgcacc gcggtgtcca tgtccgagaa gaggatgccc
     9841 tggcgctcga ggtgctcctg ggtcttgtgg tagacgacct cgctctcgta ctgagcggtg
     9901 acgccgccga ggtacttctt ctcggcctcg gggatcccga gcttgtccca cgtcgacttg
     9961 atgtcctcgg gcatgtcgtc ccaggacttc acctggttca tgggcttgat gtagtagtag
    10021 atgtcgtcga agtcgatgcc gctcaggtcg gcgccccagc ccggcatggg cttgcggtag
    10081 aagacgtcga gggccttgtg gcggaacttc cgcatccagt cgggctcgtt cttgcggtcg
    10141 ctcatcatgt cgaggacctg gtggttgatg cccttggtgg gcgccacgtc gtacgaggtg
    10201 gcgtcgtgcc agccgtactt gtactcgccc tttagggccc ggacctgctc gttctcctgc
    10261 tggaccttga ggtcctcggc ttcggtagcc actgcgttgc ctcctggggt ggatttccgg
    10321 ggcgagtcta gcagttaggt atgcctaacg tccctgctca gagggcacaa cgagggggcc
    10381 gaggggtttg ttccgggggc cgtgc
//
LOCUS       DASQDO010000154         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_76938, whole genome shotgun sequence.
ACCESSION   DASQDO010000154 DASQDO010000000
VERSION     DASQDO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76938"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1249)
                     /gene="coxB"
                     /locus_tag="VEV43_04555"
     CDS             complement(197..1249)
                     /gene="coxB"
                     /locus_tag="VEV43_04555"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYP22822.1"
                     /translation="MEPTRGGPDKAAARRARVAALLPVFALLLASCANQSPQDSLSPE
                     GPIAREQDKLWDLTFLIAVVIFFIVELVLVYAIVRFRERPGRQAAQFHGNTKLEVILT
                     VIPSLILAGIAVPTVRTIFDQAETPEGALNVKVVGKQFWWEYEYVDAGVVTANELHIP
                     TDQPVHLTLDGVATEVNGERGVIHSFWVPRLAGAQDIIPGRTLSMNIQADEPGEYEGQ
                     CKEFCGLSHANMRLRVIAHEPSEFEAWLADQQEEAAEPADSLARKGQEIFNETCIACH
                     SIQGVTEAPRNVGPDLTHFASRETFAGAMFVVTEAHVRAWMEDPPAVKPGALMPDYNL
                     SSEELDAVVAYLMGLE"
     gene            1294..>1685
                     /locus_tag="VEV43_04560"
     CDS             1294..>1685
                     /locus_tag="VEV43_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYP22823.1"
                     /translation="MVPVDELEEYPRTNRRVRLIAFSIALAMVVAALSTLLLRPGSRD
                     AADRLPSFSLAHLSGSGSLSSDDLAGSPVVLNFWASWCGPCRNEAPAFQAAWERYESE
                     GVRFVGVNVCDLEPEAKAFVREFGVTYDV"
BASE COUNT          250 a          558 c          579 g          298 t
ORIGIN      
        1 tggaggcgga tcgcgatcgc ctcgaggccc ccgaggatga agaagatgaa ggacgtgacc
       61 ccgtagagga tcccgatctt cttgtggtcg accgtcgtga tccagcccca cacccccgtg
      121 gtcgcggtgg gacggctcag aagtcctcgc cgcggggcgg ctacggttgc catctactcc
      181 actccttctg actgcttcat tcgagcccca tcagatacgc gacgacggcg tcgagctcct
      241 ccgagctgag gttgtagtcc ggcatcaacg cccccggctt cacggccggc gggtcctcca
      301 tccatgcccg tacgtgggcc tcggtcacca cgaacatcgc gccggcgaac gtctcgcggc
      361 tcgcgaagtg cgtcaggtcc gggccgacgt tgcggggggc ttccgtgacg ccctggatcg
      421 agtggcacgc gatgcaggtc tcgttgaaga tctcctggcc cttcctcgcc agcgagtcgg
      481 cgggctcggc ggcctcctcc tgctggtcgg cgagccacgc ctcgaactcg gacggctcgt
      541 gcgcgatcac gcgcaggcgc atgttggcgt ggctgagccc gcagaactcc ttgcactggc
      601 cctcgtactc gccgggctcg tcggcctgga tgttcatgct cagggtgcgg cccgggatga
      661 tgtcctgcgc ccccgccagg cgcgggaccc agaagctgtg gatcacgccg cgctcgccgt
      721 tgacctcggt cgcgaccccg tcgagcgtga ggtggacggg ctggtcggtc gggatgtgca
      781 gctcgttcgc ggtgaccacg ccggcgtcga cgtactcgta ctcccaccag aactgcttcc
      841 cgacgacctt gacgttgagc gccccctcgg gggtctcggc ctggtcgaag atcgtccgga
      901 cggtcggaac ggcgatcccc gccaggatca gcgacgggat caccgtgagg atgacctcga
      961 gcttcgtgtt gccgtggaac tgcgcggcct gccggcccgg acgctcgcgg aagcggacga
     1021 tcgcgtacac gagcacgagc tcgacgatga agaagatgac gaccgcgatg aggaacgtca
     1081 ggtcccagag cttgtcctgc tcgcgcgcga tcggtccttc gggagagagc gagtcctgag
     1141 ggctctggtt cgcgcagctc gccaggagca aagcgaatac ggggagcagg gcggcgacac
     1201 gagcgcgtct cgccgctgct ttgtcggggc ccccgcgagt cggttccaag acgagcgcaa
     1261 tatatatgaa ccccccgata ccctgaggcc gtcgtggtgc ccgtagacga gctcgaggaa
     1321 tacccgcgca cgaaccggcg agtgcggctg attgccttct caatcgcact tgcaatggtg
     1381 gtcgccgcgc tcagcactct gttgttgagg ccggggtctc gcgacgctgc ggatcgactg
     1441 ccttcgttct cgctcgcaca cctgtcgggc tcgggctcgc tgtcgtccga cgacctcgcc
     1501 gggtcgccgg tcgtcctcaa cttctgggct tcgtggtgcg ggccgtgccg caacgaggcg
     1561 ccggcgttcc aggccgcgtg ggagcggtac gagagcgagg gcgtccggtt cgtcggcgtg
     1621 aacgtgtgcg acttggagcc cgaggcgaag gccttcgtgc gggagttcgg cgtcacctac
     1681 gacgt
//
LOCUS       DASQDO010000155         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1178349, whole genome shotgun sequence.
ACCESSION   DASQDO010000155 DASQDO010000000
VERSION     DASQDO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178349"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..2157
                     /gene="glmS"
                     /locus_tag="VEV43_04565"
     CDS             322..2157
                     /gene="glmS"
                     /locus_tag="VEV43_04565"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HYP22824.1"
                     /translation="MCGIVGCVGSDDALQIMLDGLRKLEYRGYDSAGVAVVSDGDMAV
                     ERRAGRLGVLEGHLNRNWLEGSMGIGHTRWATHGAPTDANAHPHRDCTGRIAIVHNGI
                     IENHDVLRAKLEDEGHRFRSQTDTEVIAHLLESLLASGVTDLRSAVHEAVTILEGAFA
                     LVAISLDEPDKIVVARQEPPLIVGATETMGLVASDIPALLAHTRDVIPLDNRQIAEVT
                     AGRVRVFDFAGSEVTPETVHVEWDEEAAEKGGYEDFMLKEIFEQPAAVRNTMRGRFDE
                     EGRVRLDEVTWEPDVIEAIDKIVVVACGTSFHAGMAAKHSIEHWTRIPVELDLASEFR
                     YRDPVLDERSLVIGIAQSGETADTLAAVRFAKEMGASVVAVTNVVGSSMTREADAVLF
                     THAGPEIGVAATKTFTAQLVALNILALYLAQERGTMSADKRAETIAAMQRLPEQVEEV
                     LAVQGEVHRAAKCYADAKDFIFIGRGVGMAVAMEGALKLKEISYLHAEGYAAGELKHG
                     AIALIEPGVPVVAVLTGHLLYDKMLANVEEVRARGADTILIAPQGDDRARSLANELFE
                     VPATKPLLTPILDTIPTQLFAYFVCKERGLPPDKPRNLAKSVTVE"
     gene            2154..>2857
                     /locus_tag="VEV43_04570"
     CDS             2154..>2857
                     /locus_tag="VEV43_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HYP22825.1"
                     /translation="MRPLLTPEEMRRADEAAISSGVAASVLMERAGRALARSAIELAG
                     GRYGKRVLVLCGKGNNGGDGLVAARVLRGEGLGVTCVRLFEGEEPPSPERFDVVVDAI
                     FGTGFRGAPEGPVAKVIETIADHPRVVSADIPSGVDGATGAVAGAAVRAAVTVAMAAE
                     KLGTALSPGAAHAGRVVVADIGIPIPPVSTHLAEEADVARVLPSRPPGSHKKSGGSVA
                     VLAGSEAIRGAPLLTCR"
BASE COUNT          461 a          971 c         1011 g          414 t
ORIGIN      
        1 ccaggtaatt ccttccaccc tcctttcccg gcgggggctc gcgcgctaac ctggacgggc
       61 agagaggaag caccaccagt ccggatctag cgccaggtct cgccgtagga gggggcgaga
      121 cgacgaggca ggggatctcg aaaggcgttc acggaacgcc cttttcggcg ggtgaccctc
      181 ggccggccac ggtcgtgacg gtgccgacaa agcccgggag tgatcccggg ggacaaaccg
      241 gccccggcgt ggctcctctt tcgctgacca ctcatgggcg cgccctgtcg cgcccgttat
      301 accgaagagg aggagacgcc tatgtgcggc atcgtcggat gcgtaggcag cgacgacgct
      361 ctccagatca tgctcgacgg ccttcgcaag ctcgagtacc gcggttacga ctccgctggg
      421 gtcgccgtcg tctcggacgg tgacatggcc gtcgagcgca gggccggacg cctcggcgtc
      481 ctcgaaggac acctcaaccg caactggctc gaaggctcga tgggcatcgg gcacacgcgt
      541 tgggcgacgc acggcgcgcc gaccgacgcg aacgcccacc cccaccgtga ctgcacgggc
      601 cggattgcga tcgtgcacaa cggcatcatc gagaaccacg acgtcctgcg ggcgaagctc
      661 gaagacgagg ggcacaggtt ccggtcgcag accgacaccg aggtcatcgc ccacctcctc
      721 gagagcctgc tcgcgtcggg cgtaacggac ttgcggagcg cggtccacga ggccgtgacg
      781 atcctcgagg gcgcgttcgc gctcgtagcg atctccttgg acgagcccga caagatcgtc
      841 gtcgctcgcc aggagccgcc gttgatcgtc ggcgccaccg agacgatggg gcttgtcgcg
      901 tcggacatcc ccgcgctgct cgcgcacacg cgcgacgtca tcccgctcga caaccggcag
      961 atcgccgagg tgacggcggg ccgcgtccgt gtcttcgact tcgccggcag cgaggtcacg
     1021 ccggagaccg tgcacgtcga atgggacgag gaggccgccg agaagggcgg ctacgaggac
     1081 ttcatgctca aggagatctt cgagcagccc gccgcggtgc gtaacacgat gcgcgggcgc
     1141 ttcgacgaag agggccgcgt caggctcgac gaggtgacgt gggagccgga cgtcatcgag
     1201 gcgatcgaca agatcgtcgt cgtcgcctgc ggcacgtcgt tccacgcggg catggcggcc
     1261 aagcactcga tcgagcactg gacccggatc cccgtcgagc tcgacctcgc gtcggagttc
     1321 cgctaccggg accccgtcct cgacgaacgg tcgctcgtca tcggcatcgc gcagtcgggc
     1381 gagaccgccg acacgcttgc cgccgtccgc ttcgctaagg agatgggcgc gagcgtcgtc
     1441 gccgtcacga acgtcgtcgg ctcgtcgatg acgcgcgagg ccgacgcggt gctgttcacg
     1501 catgccggtc ccgagatcgg ggtcgcggcg acgaagacgt tcacggcaca actggtcgcg
     1561 ctcaacatcc tcgctcttta cctcgcgcag gagcgcggga cgatgagcgc ggacaagcgg
     1621 gccgagacga tcgccgcgat gcagcggctt cccgagcagg tcgaggaggt cctcgccgtc
     1681 caaggcgagg tgcaccgggc tgcgaagtgc tacgccgatg cgaaggactt catcttcatc
     1741 ggccgcggcg tcgggatggc ggtcgcgatg gaaggcgcgc tgaagctcaa ggagatcagc
     1801 tacctccacg ccgagggata cgcggcgggc gagctaaagc atggtgcgat cgccctgatc
     1861 gagccgggcg tgcccgtggt cgcggttctg accggtcatc ttctctacga caagatgctg
     1921 gcgaacgtcg aggaggtgcg tgcccgcggc gcggacacga tccttatcgc gccgcaagga
     1981 gacgaccgcg cgcggagcct cgccaacgag ctcttcgagg tcccggcgac gaagccgttg
     2041 ctgacgccga tcctcgacac gatcccgacg cagctgttcg cgtacttcgt gtgcaaggag
     2101 cgggggcttc cgccggacaa gccgaggaac ctcgccaaga gcgtcaccgt ggagtgagac
     2161 cgctactcac gccggaggag atgcgccgtg ccgacgaggc cgccatctcg tccggcgtag
     2221 cggcttcggt cctgatggag cgggcggggc gcgccctcgc ccgctccgcg atcgagctcg
     2281 ccggcggccg ctacggcaag cgcgtcctcg tgctgtgcgg caagggcaac aacggcggag
     2341 acggcctcgt cgcggcacgc gtcctgcgcg gagaaggcct cggcgtcacg tgcgtgcggc
     2401 tgttcgaggg cgaggagccg ccgtctcctg aacgcttcga cgtcgtcgtc gacgcgatct
     2461 tcgggacggg gttccgaggc gcgcccgaag gaccggtcgc aaaggtgatc gagaccatcg
     2521 cggaccatcc ccgggtcgtc tccgcggaca tcccctcggg agtggacggc gcgaccggag
     2581 ccgtagcggg ggccgcggtc cgtgctgccg tcaccgtcgc gatggcagcc gagaagctgg
     2641 ggaccgcgct gtcgccgggg gccgctcacg ccggacgcgt cgtcgtcgcg gacataggca
     2701 tcccgatccc gccggtgtcg acgcatctcg ccgaggaggc cgacgtcgcg cgggtgctgc
     2761 cgtcccggcc ccccggctcc cacaagaaga gcgggggttc ggtcgccgtc ctcgccggct
     2821 ccgaggccat ccgcggtgcg ccgttgctca cgtgccg
//
LOCUS       DASQDO010000156         6146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_811411, whole genome shotgun sequence.
ACCESSION   DASQDO010000156 DASQDO010000000
VERSION     DASQDO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811411"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VEV43_04575"
     CDS             <1..324
                     /locus_tag="VEV43_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HYP22826.1"
                     /translation="GEGAEWAPEGCVGYSKICTHAGCPVGLYRAQAHELLCPCHQSTF
                     DVLRGAVPTFGPADRPLPQLPRGVDDEGVLIALGDFPEPVGPGFWNLRDGRSSGRSAS
                     AKEDR"
     gene            324..1685
                     /locus_tag="VEV43_04580"
     CDS             324..1685
                     /locus_tag="VEV43_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HYP22827.1"
                     /translation="MLRWLDSRLGAASWARKALRKVFPDHWSFLLGEIALFCFLILLA
                     TGIFLTLFYRADATRVIYDGPYVPLQGREVSAAFESVMRISYEVRAGLVMRQIHHWAA
                     LVFVASIVVHTMRVFFTGAFRKPREVNWLVGIGLLILAIGMGFTGYSLPDDLLSGTGL
                     RIAYSVLISIPLVGPYLAFLVFGGEFPTEGIISRLFVLHVMLLPAAIIGLITAHLGIL
                     VRQKHAQHRGPGRTERNVVGKALWPSQVFKSVGLLALTASVLAFMGGLFQINPVWDFG
                     PFDATTVSAASQPDWYVGWLEGALRLFPPFDLEVFGVLIPSPFIPGVVIPGLGFTVLA
                     LWPFIEARITGDTDEHHLLDRPRDAPLRTAVGVAGLLYFGVLTIAGGNDVLALLFNVP
                     VETMTVLLRYATFVLPVFGGLLAYRIASDLGSSGSHPFRRSRGVKLRRTESGGFEETE
                     AGG"
     gene            1772..2437
                     /locus_tag="VEV43_04585"
     CDS             1772..2437
                     /locus_tag="VEV43_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP22828.1"
                     /translation="MHGIGASGRYFMPLARRLAERNRVYAPDFPGFGRSAKPRRALTL
                     QDLAGVLDEWMGHLSLGSAVMVGNSFGCQIVAELALRHPARLEAAAFLGPTIDRRARN
                     AWSQAGRVVATAVREPAGLVRPLLADYLQAGIVRPLQTLRYALDDPLEAKLPHVDVPV
                     LVVRGDGDAIVPQSWAEEVARLLPLGELVVVPGGAHVLNYDAAGAIADLLERAWNPAP
                     DSG"
     gene            2680..3966
                     /locus_tag="VEV43_04590"
     CDS             2680..3966
                     /locus_tag="VEV43_04590"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22829.1"
                     /translation="MWASRSPRRPRPSPESTELGPETPGLANLVAHRSNPTRDAVPAT
                     LVDLAARGLIEIEDRGIGRYYCRLTGKAAELTPYERMLVDHLRSLAADGVIPAEALTT
                     GPQDRSKAWWDEFRKKLVAHAQSAGLCTNLWNATAISLLVGGGVVLLVLYALATGFND
                     PDDVADSPLLNAVTLGALPAIFALGAVVKSTRQTSTAEGLRCGSRWLGVRRALEESPS
                     FEQLPPSGVVVWERHLAYAAALGVAPRSVRALPMGAESDTEVWARAGTEWRKVEVRYP
                     RFRPGWGRHPLHALGVGFLGTLFARWLLRLATGDLGGDDTWLKAIALVLFGIAVLVLT
                     RSLPQLVLGFLDLLASRPVEGTILRARTRWSPFPYSFQGSDQQYLRYFVAIDDGRSPK
                     LTAYRVKPSLYGALSQGTEAKLKVTPNLGYVRRLSA"
     gene            complement(3947..4297)
                     /locus_tag="VEV43_04595"
     CDS             complement(3947..4297)
                     /locus_tag="VEV43_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22830.1"
                     /translation="MPEELRGPAGVALYVAVATVVYWLVSDEIAGTLLLVFLFVAAVA
                     LVLLGLALRRPGVDPALEIEEEPVVTVSPWPLLGAAAAMLVGLGLLYGPWLWIPGAAL
                     GAGSAYGWITQTDA"
     gene            complement(4290..5909)
                     /gene="ctaD"
                     /locus_tag="VEV43_04600"
     CDS             complement(4290..5909)
                     /gene="ctaD"
                     /locus_tag="VEV43_04600"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HYP22831.1"
                     /translation="MPASEAAAVAAHPEPRGLVAWLTTTDHKRIGLLYLWSALIFFVA
                     GGVMSLLMRAELAQPGLQLLERETYNQLFTMHGTAMIFFFATPVAVGLANYLVPLQIG
                     APDVAYPRLNALTFWLFLAGALTAFSGFLTASGAAAVGWTGYAPLSESTYSPGAGVDL
                     WIIGLLLSGMASILGAVNFLATIFGMRAPGMTMFRVPIFTWNIVAISVLILFSFPVLT
                     AALAALFVDRNFGGHFFDPSQGGNPVLWQHLFWFFGHPEVYIVILPFFGVVSEIVPVF
                     SRKPLFGYKGFVFATLLITAYSFSVWAHHMFTTGAVSSLFFATTSFLIAIPTGVKFFN
                     WIATMWRGKLTFEAPMLFCAGFLFMFLIGGISGVFLASPPIDYAVHDTYYVVAHMHYV
                     LFGGTTFAAFAALYFWIPKFSGRLMGEGLARVHFVLMLVGMNLAFFPMHQLGLDGMQR
                     RVPDYPESSGYGDLNMLSTVGAFLIAVSVVVFAVNFAVSMRRGRSAGGDPWGGYSLEW
                     ATTSPPPHHNFESLPPVRSERPVYDARTGDA"
     gene            complement(5896..>6146)
                     /locus_tag="VEV43_04605"
     CDS             complement(5896..>6146)
                     /locus_tag="VEV43_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYP22832.1"
                     /translation="LRSRDVVHSFYVPRFLVKRDTIPGETNRIDYLIREEGTYDGRCA
                     EFCGVLHDSMPFTIEAVSMDEFEGWLAEQRTENEDAGI"
BASE COUNT          953 a         2129 c         2145 g          919 t
ORIGIN      
        1 ggcgaggggg ccgagtgggc tcccgaaggg tgcgtcggct attcgaagat ctgcacgcac
       61 gcgggctgtc cggtcggcct ctaccgggcc caggcgcacg agctcctctg cccgtgccac
      121 cagtcgacgt tcgacgtgct ccgcggcgcg gtgcccacgt tcgggcccgc cgaccggccg
      181 ctgccgcagc tcccgagggg agtcgacgac gaaggggtcc tgatcgcgct cggcgacttc
      241 cccgagccgg tggggccggg cttctggaac ctccgggacg gccgatcgtc tggacgatcg
      301 gccagcgcaa aggaagaccg ctgatgctgc gctggctcga ctcccgcctc ggtgcggcct
      361 cgtgggcccg caaggccctg cgcaaggtct tcccggacca ctggtcgttc ctgctcggcg
      421 agatcgcgct gttctgcttc ttgatcctgc tcgcgacggg gatcttcctg accctgttct
      481 accgggccga cgcgacgcgg gtgatctacg acgggccgta cgtcccgctg cagggccgcg
      541 aggtctcggc cgcgttcgag tccgtcatgc gcatctcgta cgaggtgcgg gccggcctcg
      601 tcatgcggca gatccaccac tgggccgcgc tcgtgttcgt cgcgtccatc gtcgtgcaca
      661 cgatgcgcgt gttcttcacc ggggcgttcc gcaagccgcg cgaggtcaac tggctcgtcg
      721 gcataggcct cttgatcctc gcgatcggga tggggttcac cggctactcg ctgccggacg
      781 acctgctgtc gggaacgggg ttgcggatcg cgtactcggt gctgatctcg atcccgctcg
      841 tcgggccgta cctcgcgttc ctcgtgttcg gcggcgagtt cccgaccgag gggatcatct
      901 cgcgcctgtt cgtgctgcac gtgatgcttc tccccgcggc gatcatcggt ctcatcaccg
      961 cgcacctcgg catcctcgtg cggcagaagc acgcgcagca tcgggggccc ggccggaccg
     1021 agcgcaacgt cgtcggcaag gcgctgtggc cgtcgcaggt cttcaagtcg gtaggtctgc
     1081 tcgcgctcac cgcgtcggtc ctcgcgttca tgggcgggct gttccagatc aaccccgtct
     1141 gggacttcgg cccgttcgac gcgacgacgg tcagcgcggc gtcgcagccg gactggtacg
     1201 tcggctggct cgagggcgcg ctccgcctgt tcccgccgtt cgacttggag gtgttcgggg
     1261 tgctgatccc gtcgccgttc atcccgggcg tcgtgatccc ggggctgggc ttcaccgtgc
     1321 tcgcgctgtg gccgttcatc gaggcgcgga tcacgggcga caccgacgag caccacctgc
     1381 tcgaccggcc gcgcgacgcg ccgctgcgca ccgcggtcgg cgtcgcgggg ctcctctact
     1441 tcggcgtgct gacgatcgcc gggggcaacg acgtgctggc gctgctgttc aacgtcccgg
     1501 tcgagacgat gacggtgctg cttcgctacg cgacgttcgt gctcccggtg ttcgggggcc
     1561 tgctcgcgta ccggatagcg agcgacctcg ggagctcggg ctcgcacccg ttccggcgct
     1621 cgcgcggcgt gaagctgcgc cggacggaga gcgggggctt cgaagagacc gaggccggcg
     1681 gctgaggttc gactcggtct acacgcagac gtcgcgcggg ctcgtccatg cgcgcgtctc
     1741 ggatcgtcgc gggggccgga cgttcgtcct cgtgcacggc atcggcgcgt ccggccgtta
     1801 cttcatgccg ctcgcccggc ggctggccga gcgcaaccgc gtctacgccc ccgacttccc
     1861 cggcttcggg cgcagcgcca agccccggcg cgcgctgacg ttgcaggatc tcgccggcgt
     1921 cctcgacgag tggatggggc acctctcgct cgggagcgcc gtgatggtcg gcaactcgtt
     1981 cggctgccag atcgtcgccg agctcgcgct gcgtcacccg gcacgcctcg aggcagcggc
     2041 cttcctgggc ccgaccatcg accgccgggc ccgcaacgcg tggtcgcagg cgggccgggt
     2101 cgtcgcgact gccgtgcggg aaccggccgg gctcgtgcgg ccgctgctcg cggactacct
     2161 gcaggccggc atcgtgcgcc cgctccagac gctgcggtac gcgctggacg acccgctcga
     2221 ggccaagctc ccgcacgtag acgtccccgt cctcgtcgtg cgcggcgacg gggacgcgat
     2281 cgtgccgcag agctgggccg aggaggtcgc ccggctgctg cccttgggcg agctcgtggt
     2341 cgtccccggc ggggcccacg tcctcaacta cgacgcggca ggggcgatcg ccgatctgct
     2401 cgaacgagct tggaatccgg cgcccgacag cggatgatgg gggccccacc cgaggcgcag
     2461 gaggtcccgt ggcgaacaag gacaagggcg gcggcaaggc cgacaagaag cagtcgcaga
     2521 agagcctgaa ggagaagcgg gcggcgaaga agcagaagaa gggcaagacg ccgcccatga
     2581 cgccctgacc aacctctgaa cgagctctac gtcccccacg agggacttct ccgcgcgacc
     2641 gcggccggcc tcgtcgcgtg gttcgcgctg ctcggggcct tgtgggcgtc gcgttcgccg
     2701 cggcggccgc ggccgagccc ggagtcgacc gagctcgggc ccgagacccc cgggctggcg
     2761 aacctcgtcg cccaccgctc gaacccgacg cgcgacgcgg tcccggcgac gctcgtcgac
     2821 ctcgccgcgc gcgggctgat cgagatcgag gaccggggca tcggcaggta ctactgccgg
     2881 ctcacaggca aggcggccga gctgacgccc tacgagcgga tgctcgtcga ccatctgcgg
     2941 tcgctggcgg cggacggcgt gatcccggcc gaggcgctga cgaccggccc gcaggaccgt
     3001 tcgaaggcgt ggtgggacga gttccgcaag aagctcgtcg cgcacgcgca gtccgcgggg
     3061 ctgtgcacca acctgtggaa cgcaacggcc atctcgttac tggtcggagg cggtgtggtg
     3121 ctcctcgtgc tgtacgcgct cgctaccggc ttcaacgacc ccgatgacgt ggcggactcg
     3181 cctctgctga acgcggtgac gctgggagcg ctgccggcga tcttcgccct gggcgccgtg
     3241 gtcaagtcga cgcgccagac ctcgaccgca gagggattgc gctgcggctc tcgctggctg
     3301 ggagtccggc gcgcgctgga ggagagcccg tcgttcgagc agctgccgcc gagcggcgtc
     3361 gtcgtttggg aacgccacct tgcgtacgcg gccgcgctgg gtgtggcgcc ccgctccgtc
     3421 agggcgctac ccatgggggc cgagagcgac accgaggtct gggcccgcgc cggcaccgag
     3481 tggcggaagg tggaggtgag gtacccgagg ttccggccgg gctggggccg tcatcccctt
     3541 cacgcgctgg gagtcgggtt cctggggacg ctgttcgccc ggtggctgct gaggctggcg
     3601 acgggcgacc tgggaggcga cgacacgtgg ctgaaggcca tcgctctcgt tcttttcggg
     3661 atagcggtgc tggtgctgac gcgctcgctc ccgcaactcg tgttgggctt cctggacctg
     3721 ctcgcgtccc ggccggtgga gggaacgatc ctgcgggcga ggacgaggtg gtcgccgttc
     3781 ccgtactcgt tccagggctc ggaccagcaa tacctgcgct acttcgtggc gatagacgac
     3841 ggccgcagcc cgaagctgac ggcctaccgg gtcaagccgt cgctatacgg agcgctgagc
     3901 caaggaacgg aggcgaagct gaaggtgacg ccgaacctcg gctacgtcag gcgtctgtct
     3961 gcgtgatcca gccgtaggcc gagccggccc ccagcgcggc tccgggtatc catagccacg
     4021 gcccgtacag caggcccagc cccaccagca tcgccgcggc cgctcccagg aggggccacg
     4081 ggctcaccgt cacgaccggt tcctcttcta tctccaaagc cgggtccacc cccgggcggc
     4141 gcagcgccag gccgaggagg accagagcca cggccgctac gaagaggaag accagtaaca
     4201 acgtcccggc tatctcgtcc gacacaagcc agtacaccac cgtcgcgacg gcgacgtaga
     4261 gcgcgacgcc ggctgggccc ctcagctcct caggcatcgc cggtcctcgc gtcgtatacc
     4321 gggcgctccg aacggacggg agggagcgat tcgaagttgt ggtgcggcgg cggcgatgtc
     4381 gtcgcccatt ccaacgagta gccgccccag gggtcgcccc cggcagagcg gccccgtctc
     4441 atcgagacgg cgaagttcac ggcgaacacg acgacggaca ccgcgatcag gaacgccccg
     4501 accgtgctca gcatgttcag gtcgccgtag cccgacgact cgggatagtc gggcacgcgg
     4561 cgctgcatcc cgtcgaggcc cagctggtgc atcgggaaga acgccaggtt catccccacg
     4621 agcatcagca cgaagtggac ccgagcgagg ccctcgccca tcagccgccc actgaacttc
     4681 gggatccaga agtagagggc cgcgaacgcg gcgaacgtcg tcccaccgaa cagcacgtag
     4741 tgcatgtgcg cgaccacgta gtacgtgtcg tgcaccgcgt agtcgatcgg cggcgacgcg
     4801 aggaacacgc cggagatccc cccgatcagg aacatgaaca ggaaccccgc gcagaacagc
     4861 atgggggcct cgaacgtgag cttcccgcgc cacatcgtcg cgatccagtt gaagaacttc
     4921 accccggtcg ggatagcgat gaggaacgac gtcgtggcaa agaacaggct cgatacggct
     4981 cccgtcgtga acatgtggtg cgcccacacc gagaagctgt acgcggtgat cagcagcgtc
     5041 gcgaagacga agcccttgta gccgaacagc ggcttgcgcg agaacaccgg cacgatctcc
     5101 gagaccacgc cgaagaacgg aaggatcacg atgtagacct cgggatggcc gaagaaccag
     5161 aacaggtgct gccacaggac cggattccca ccctgggacg ggtcgaagaa gtgcccgccg
     5221 aagttccggt cgacgaacag cgcggccagc gcggcggtga gaacggggaa cgagaacagg
     5281 atcaggaccg agatcgccac gatgttccac gtgaagatcg gcacccggaa catcgtcatc
     5341 cccggcgccc gcatcccgaa gatcgtcgcg aggaagttca ccgcgccgag gatcgaggcc
     5401 atccccgaca ggagcagccc gatgatccac aggtcgaccc cggcccccgg cgagtacgtc
     5461 gactcgctca acggcgcata gccggtccac ccgacggccg cggccccgct cgcggtcagg
     5521 aagcccgaga acgccgtcag ggccccggcc aggaagagcc agaacgtgag cgcgttgagc
     5581 cgcgggtacg cgacgtcggg cgccccgatc tgcaacggca cgaggtagtt cgcgagcccg
     5641 accgcaaccg gcgtcgcgaa gaagaagatc atcgcggtgc cgtgcatcgt gaacagctgg
     5701 ttgtaggtct cgcgctcgag gagctggagg ccgggctgcg cgagctccgc ccgcatcagc
     5761 aaggacatca ccccgccggc gacgaagaag atcagcgcgc tccacaggta gaggagcccg
     5821 atcctcttgt ggtcggtcgt cgtcagccac gcgacgagcc cccgtggctc ggggtgggcc
     5881 gcgaccgccg ccgcctcaga tgccggcatc ttcgttctcc gtcctctgct ccgcgagcca
     5941 tccctcgaac tcgtccatcg agaccgcctc gatcgtgaaa ggcatcgagt cgtggagcac
     6001 gccgcagaac tcggcgcacc gcccgtcgta cgtcccctcc tcgcggatga ggtagtcgat
     6061 cctgttcgtc tcgccgggga tcgtgtcgcg cttcacgagg aagcgcggca cgtagaacga
     6121 gtgcacgacg tcgcgcgagc gcaggt
//
LOCUS       DASQDO010000157         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_750736, whole genome shotgun sequence.
ACCESSION   DASQDO010000157 DASQDO010000000
VERSION     DASQDO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750736"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1932
                     /locus_tag="VEV43_04610"
     CDS             <1..1932
                     /locus_tag="VEV43_04610"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HYP22833.1"
                     /translation="LAEVEAELGFEVAQLIDGVTKLDRMRLRSAEHGRAENLRKMIVA
                     TAKDVRVLLVKIADRLHNMQTLAPLAPHKRELIAMETLEIYAPLAHRLGMYAVKWQLE
                     DLAFKTLHPKRYAEIEGLVQQRQPERERVLDEVVTQILDKLRDMKIKAEVFGRPKNLY
                     AIYEKVVLRGKQFEEIFDLVGIRVVVESVRDCYAALGALHTLFTPVPGRFKDYIAKPK
                     FNMYQSLHTTVVGPGGRAMEIQIRTQEMHRAAEIGIAAHWLYKEQRRGKVSEEDLAWL
                     QRMMDWQKESEDPKDFMESLKMDLYADEVFAFTPKGDVLELPRGATPIDFAYSIHTEV
                     GHRTVGAHVNGRIVPLGYELNSGDTVQIITTKGPAAPSRDWLQLVKTPRARNKIRQHF
                     ARERRDDALAQGRDALVSAMRKQGLPVDKVTKGDLLRQISEELKYPDLDGMFVALGAG
                     HLSPQTVTTRVIRLFQPEPETEPAEIEEPPVRRRSRVPRGKGVIVEGVEDLMVRLARC
                     CTPVPGDPIVGFLTRGRGVSVHRDDCPNAKALQVTDEGRIAKVWWDDRQQGTFIVGIQ
                     IEAMDRAKLLRDVTTAISDQGLHIVASSTRTGRDGIATLTFTFELADPSHLEHVIQSV
                     RRVDSVFDAYRTVPASART"
     gene            1943..>2840
                     /locus_tag="VEV43_04615"
     CDS             1943..>2840
                     /locus_tag="VEV43_04615"
                     /inference="COORDINATES: protein motif:HMM:NF017073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HYP22834.1"
                     /translation="MKRALASLVVVALLAPACTPEDEGPELPPAGAAFESFVDAWNER
                     DASAMSELMTDPSGGLGRFFGEDLGDEVEFEVTPGDVATQTTTPEDASPTATSTAQYS
                     IAYSVDGSEGEPLDGELEMRLQGGEWLVDWEEASMWPGFEDADGLRVVRKWPPRAPIR
                     DRKGRVLATGPAPGRRYPFAATAGSTVGHIGSLTRKEAAEGDVYEEGDLAGASGMELA
                     FDERLTGTPGLRLDVVDGKKRLETLARAAPDPGRPVRTTLDVRIQQAAAGAFGGTTGG
                     AVVLDPANGDLLAVVSSAELNPA"
BASE COUNT          480 a          997 c          984 g          379 t
ORIGIN      
        1 ctggccgagg tcgaggccga gctcggcttc gaggtcgcgc agctgatcga cggggtcacg
       61 aagctcgaca ggatgcgcct gcgttccgcg gagcacgggc gggccgagaa cctccgcaag
      121 atgatcgtgg cgacggccaa ggacgtccgc gtcctgctcg tgaagatcgc cgaccgcctc
      181 cacaacatgc agacgctcgc gccgctcgca ccgcacaaac gagagctgat cgcgatggag
      241 acgctcgaga tctacgcgcc gctcgcgcac cggctcggca tgtacgcggt gaagtggcag
      301 ctcgaggacc tcgcgttcaa gacgctgcac cccaagcgct acgccgagat cgaggggctc
      361 gtccagcagc gccagcccga gcgagagcgg gtgctcgacg aggtcgtcac gcagatcctc
      421 gacaagttga gggacatgaa gatcaaggcg gaggtcttcg ggcgtcccaa gaacctctac
      481 gcgatctacg agaaggtcgt gctgcgcggc aagcagttcg aggagatctt cgacctcgtc
      541 ggcatccgcg tcgtcgtcga gtcggtcagg gactgctacg cggccctcgg cgcgctccac
      601 acgctgttca cgccggtccc cggccgcttc aaggactaca tcgcgaagcc gaagttcaac
      661 atgtaccagt cgctgcacac cacggtcgtg ggcccggggg gccgcgcgat ggagatccag
      721 atccgcacgc aggagatgca ccgcgccgcc gagatcggga tcgccgcgca ctggctctac
      781 aaggagcagc gccgcgggaa ggtctccgag gaggacctcg cctggctcca gcggatgatg
      841 gactggcaga aggagtccga ggaccccaag gacttcatgg agtccttgaa gatggacctc
      901 tacgcggacg aggtcttcgc cttcacgccg aagggcgacg tcctcgagct cccgcgcggc
      961 gcgacgccga tcgacttcgc ctactcgatc cacaccgagg tcggccaccg cacggtggga
     1021 gcacacgtca acgggcgcat cgtcccgctc ggctacgagc tcaactccgg cgacaccgtc
     1081 cagatcatca ccacgaaggg cccggccgcg ccctcgcgcg actggctcca gctcgtcaag
     1141 acgccgcgtg cccgcaacaa gatcagacag cacttcgccc gtgagcgccg cgacgacgcg
     1201 ctcgcccagg gacgcgacgc gctcgtcagc gcgatgcgca agcagggcct gcccgtcgac
     1261 aaggtgacga agggcgacct gctccggcag atctccgaag agctgaagta cccggacctc
     1321 gacgggatgt tcgtcgcgct gggggccggg cacctctcgc cgcagacggt caccacgcgc
     1381 gtgatccgtc tgttccagcc ggagccggag accgagcccg cagagatcga ggagcctccg
     1441 gtacgccgcc gctcgcgcgt cccgcgcggc aagggcgtga tcgtcgaggg cgtggaagac
     1501 ctgatggtgc gcctcgcgcg ttgctgcacg ccggtgccgg gcgaccccat cgtgggtttc
     1561 ctcaccaggg ggcggggggt atcggtccac cgggacgact gccccaacgc gaaggcgctg
     1621 caggtgaccg acgagggaag gatcgcaaag gtttggtggg acgaccggca gcagggcacg
     1681 ttcatcgtcg gcatccagat cgaggccatg gaccgcgcga agctgctgcg cgacgtcacg
     1741 acggcgatct ccgaccaggg gctgcacatc gtcgcgtcgt cgacgcgtac gggccgggac
     1801 gggatcgcga cgctgacgtt cacgttcgag ctcgccgacc cgtcgcacct cgaacacgtc
     1861 atccagagcg tccgacgggt ggactcggtc ttcgacgcgt accgcacggt cccggcctcg
     1921 gcgcgtacct gacccacccg tagtgaagcg cgccctcgca tcgctcgtcg tcgtcgcgct
     1981 gctcgcgcct gcgtgcaccc cggaggacga ggggccggag ctcccacccg ccggcgcggc
     2041 gttcgagtcg ttcgtcgacg cctggaacga gcgggacgcc agcgccatga gcgagctgat
     2101 gaccgatccg agcggcggtc tcggccggtt cttcggcgaa gacctcggag acgaggtcga
     2161 gttcgaggtc acccccggtg acgtcgccac gcagaccacg acgcccgagg acgcgtcgcc
     2221 gaccgcgacc agcaccgcgc agtattcgat cgcctactcg gtcgacggct ccgaaggcga
     2281 gccgctcgac ggcgagctgg agatgcggct gcagggcggc gagtggctcg tcgactggga
     2341 ggaagcgtcg atgtggcccg ggttcgaaga cgccgacggg ctgcgcgtcg tccgcaagtg
     2401 gcctccgcgt gcgcccatcc gcgaccggaa gggcagggtc cttgccaccg gcccggcccc
     2461 cgggcgccgt tacccgttcg ccgccaccgc cggcagcacc gtgggacaca tcggctcgct
     2521 cacccgcaag gaggccgcgg agggcgacgt gtacgaagag ggcgacctcg ccggcgcgtc
     2581 cggcatggag ctcgcgttcg acgaacgcct caccgggacg ccgggtctcc ggctcgacgt
     2641 cgtcgacggg aagaagcgcc tggagacgct ggcgcgcgcg gcccccgacc ccgggcgccc
     2701 cgtccgcacg acgctcgacg tccgcatcca gcaggccgct gcgggagcat tcggcgggac
     2761 cacgggagga gccgtcgtcc tcgacccggc gaacggtgac ctcctcgcgg tcgtctcgtc
     2821 ggcggagctc aaccccgcga
//
LOCUS       DASQDO010000158         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_628410, whole genome shotgun sequence.
ACCESSION   DASQDO010000158 DASQDO010000000
VERSION     DASQDO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628410"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VEV43_04620"
     CDS             complement(<1..946)
                     /locus_tag="VEV43_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22835.1"
                     /translation="MPASGRPSGDPAPAPTFDDYLKGHLTHPAVQQAERFVEKQEALG
                     TRPTALQGDTYGRPGCETPAEYEVGAERTFWVAQNQAGNQQIQAVLAAKSKHGYMWVQ
                     REFYLPTAGEAPEGGFVTQGEAEAGLVDWESIYEIDRKYFGIEPNPKSPAQNLPPGLP
                     SNWRDADCDVHVHILNFPIDAGNGVGYTAGYYSSEHEYPNGDGEHQSPFSNEAEMFFM
                     NSLFLNVGDDTYAGVLAHEFFHMIQFSNDYNEETWVNEGMADVAAVVNGFGDIVQGHI
                     DAYQDNPDQHLFDWTGVVDDYGQAFLFFDYLFNHYGAPE"
     gene            complement(1070..2563)
                     /locus_tag="VEV43_04625"
     CDS             complement(1070..2563)
                     /locus_tag="VEV43_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYP22836.1"
                     /translation="MDERPERAPATSLLATALVPCAVIVGIVFRVWMLASPIGMLDSD
                     EAVVGLMARHMLEGEFPVFFWGQAYGGPHEAALAAAVFSVAGSSVVALKLVPAALAAV
                     AAALTWRVGRRTVGEPAASIGATLMWVWPMAFVWWSIKAVGFYQAGLVAALGVVLCAL
                     RLRERNSWIDAALLGLCAGTAWWATAQSVYLMLPTIAWLVAVRPRVLKVAPVALAGAL
                     VAAGPWLVHNARNAWVGLDAPDPGIPGNTYFDHLRVFFTEALPRALGLRLPDGWLPPG
                     GAHVAYALLLALFVYAAVRLRGNARLLVLIAVAYPFLFALSPFSWYVEHPRYLYFLAP
                     VPALLIARGLTKIPVAAAVAAFATMLGLTVVALVSVNRDARMIPPAPEIRSPLPVAPV
                     VEHMEEQGVEAAWATYWLAYRLTFESGEDVIVTPYSGIMRNAEYADYVRNHPEAAFVF
                     LDESSMDDHFSSALEGMGIAYDRTAVEGFVVYRPHADVDPEAVPGLL"
     gene            complement(2573..3847)
                     /locus_tag="VEV43_04630"
     CDS             complement(2573..3847)
                     /locus_tag="VEV43_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP22837.1"
                     /translation="MSAPVSGRTKVRQLLAQRDLRTLLSAQWTAQAADGLAQVAFAEA
                     LILGETPGRILVAFAITLLPYSLLAPFLGVFVDRWRRRELMAATNAARAVVLVTLPLW
                     AGLMPDDAALYVGVLVVLGLGRLFLVTKGAVLPVVLEEHHLLRGNALSSGGGMISALL
                     GGVVGVGVVGWLGPRPSFAAAGLFYGGAAWITRRISRSFAHPHAPDETFGAAARRVVR
                     ELAEGVVEILRRPRARIPLAAIFVLRTIGMIVAIAAILVITSEFPDAGDRFGRLSSGA
                     LALGSAGAGAFLGAVTAPAIGRRLAKPGLVVAGFVVSGVGIAALGGLRSIAAVLALTA
                     VGGYGGFLTKVAVDAQVQEALPDEYRGRAFSLYDILYNLASVAAAGVMVLGQEVSLRP
                     MFAGTGAVTLVLAAAFAAVMARHGMRLVRVRR"
     gene            complement(3873..5018)
                     /locus_tag="VEV43_04635"
     CDS             complement(3873..5018)
                     /locus_tag="VEV43_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HYP22838.1"
                     /translation="MAKVRVGIVGVGNCASSLVQGIEYYAKAKEDEFVPGLMHVQLGG
                     YHVSDVEFTCAFDVDSNKVGLDLADAIWAEPNNTIKFSDVPQTGIEVLRGHTYDGLGH
                     YYREVVTESELPPVDVARVLRETQTDVLVSYLPVGSEQAARFYAEQALEAGVGFVNCM
                     PVFIATNEEWAQRFVKKGVPIIGDDIKSQLGATISHRVLSKLFENRGVTIDHTYQLNF
                     GGNMDFMNMLERSRLQSKKISKTQSVTSQIENGIDKEDIHIGPSDHVPWLKDRKWAYI
                     RIEGREFGDIPVNIELKLEVWDSPNSAGVVIDAVRCVKLALDRGIGGPLLAPSAYFMK
                     SPPIQYTDSEAHELVEEFIRGDLGGAAGSNGVASFSLDADPALKRRA"
     gene            complement(5051..>5288)
                     /locus_tag="VEV43_04640"
     CDS             complement(5051..>5288)
                     /locus_tag="VEV43_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYP22839.1"
                     /translation="YLQPETRLALLERRRAYLQEKLAQVKANLRDYRERIDSYTLSLQ
                     NHGMAATENDIAWIDELIREERSLEAGRANPSGI"
BASE COUNT          848 a         1835 c         1850 g          755 t
ORIGIN      
        1 cctcgggcgc cccgtagtgg ttgaagaggt agtcgaagaa caggaacgcc tggccgtagt
       61 cgtcgaccac gccggtccag tcgaacaggt gctggtccgg gttgtcctgg taggcgtcga
      121 tgtggccctg cacgatgtcg ccgaagccgt tcacgacggc ggcgacgtcg gccatgccct
      181 cgttgaccca ggtctcctcg ttgtagtcgt tcgagaactg gatcatgtgg aagaactcgt
      241 gcgcgagcac ccctgcatac gtgtcgtcgc cgacgttgag gaacagcgag ttcatgaaga
      301 acatctcggc ctcgttgctg aacggggact ggtgctcgcc gtcaccgttc gggtactcgt
      361 gctcggacga gtagtagccc gcggtgtacc cgacgccgtt ccccgcgtcg atcgggaagt
      421 tcaggatgtg gacgtggacg tcgcagtccg cgtcgcgcca gttcgacgga aggcccggcg
      481 ggaggttctg cgcggggctc ttcgggttcg gctcgatccc gaagtacttg cggtcgatct
      541 cgtagatgct ctcccagtcg acgagcccgg cctcggcctc gccctgcgtg acgaaccctc
      601 cctcgggggc ctcgcccgcc gtcgggaggt agaactcgcg ctgcacccac atgtagccgt
      661 gcttggactt cgccgcgagc actgcctgga tctgctggtt gccggcctgg ttctgcgcga
      721 cccagaacgt gcgctcggcc cccacctcgt actcggcggg agtctcgcag ccgggacgcc
      781 cgtacgtgtc gccctgcagc gctgtcggcc gcgtcccgag ggcctcctgc ttctcgacga
      841 agcgctccgc ctgctggacg gcggggtgag tgaggtgacc tttcaggtag tcgtcgaacg
      901 tcggggccgg cgcaggatcg ccggaaggac gtccggacgc gggcacggcg ccgagcagac
      961 acagcgcgag aacgaggaca gagacgcgtc gcagcaaaag aagacccccc agtggacgat
     1021 tccggcatta cgtacaacct cccccgggcg ggggatgttc ctccgaggac tagaggaggc
     1081 cgggaacggc ctcggggtcg acgtcggcgt ggggccggta gacgacgaag ccctcgacgg
     1141 ccgtgcggtc gtacgcgatc cccatccctt ccagcgcgct ggagaagtgg tcgtccatcg
     1201 aggactcgtc gaggaacacg aacgctgcct ccggatggtt gcgcacgtag tccgcgtact
     1261 ccgcgttgcg catgatcccg gagtacggcg tgacgatgac gtcctcgccg ctctcgaacg
     1321 tcaggcggta cgcgagccag tacgtggccc acgcggcctc caccccttgc tcctccatgt
     1381 gctcgaccac gggcgcgacc ggcagcggcg agcggatctc cggcgcgggc gggatcatgc
     1441 gcgcgtcccg gttcaccgag acgagcgcga ccaccgtgag gccgagcatc gtcgcgaacg
     1501 cagcgacggc cgccgccacg gggatcttcg tcagccctcg cgcgatcagg agcgcgggca
     1561 cgggcgccag gaagtagagg tagcgcgggt gctcgacgta ccaggagaac ggcgacagcg
     1621 cgaacaggaa cggatacgcg acggcgatca gcacgagcaa tcgggcgttg ccgcgcagcc
     1681 ggacggcggc gtacacgaac agtgcgagga ggagcgcgta ggcgacgtgt gctccacccg
     1741 gcggcagcca cccgtccggc agccgcagcc ccagcgcccg cgggagcgcc tcggtgaaga
     1801 acacccgcag gtggtcgaag taggtgttcc cggggatccc ggggtcgggc gcgtcgagcc
     1861 ccacccaggc gttgcgcgcg ttgtggacga gccacggccc cgcggcgacg agcgctccgg
     1921 cgagggcgac gggcgcgacc ttgagcacgc gcggccgcac cgcgaccagc cacgcgatcg
     1981 tcggaagcat caggtagacg ctctgcgcgg tagcccacca cgcggtcccc gcgcagaggc
     2041 cgaggagcgc cgcgtcgatc cacgagttgc gctcgcgcag ccgcaacgcg cagaggacga
     2101 cgcccagggc cgcgacgagg cccgcctggt agaagccgac tgccttgatc gaccaccaca
     2161 cgaacgccat cggccagacc cacatgaggg tcgcgccgat cgacgccgcg ggctcgccga
     2221 ccgtccggcg cccgaccctc caggtgagcg cggccgcgac cgcggccagc gccgcgggca
     2281 cgagcttgag cgcgacgacg gacgagccgg cgaccgagaa gaccgccgcg gcgagcgcag
     2341 cctcgtgggg ccctccgtac gcctgacccc agaagaagac cgggaactcg ccttcgagca
     2401 tgtgccgggc catcagcccg acgaccgcct cgtcggagtc gagcatgcct atgggcgacg
     2461 cgagcatcca gacgcggaac acgatgccga cgatgaccgc gcacggcacg agggccgtcg
     2521 cgagcaggga ggtggcgggg gcgcgctccg gccgttcgtc cacgccggga gcctagcggc
     2581 ggaccctgac gaggcgcatc ccgtggcgcg ccatgaccgc cgcgaaggcc gcggcgagca
     2641 ccagcgtgac ggcccccgtc ccggcgaaca tcggccgcag cgacacctcc tgaccgagca
     2701 ccatgacgcc cgcggcggcg acgctcgcga ggttgtagag gatgtcgtag agcgagaacg
     2761 cgcggcccct gtactcgtcg gggagcgcct cctgcacctg ggcgtcgacc gcgaccttcg
     2821 tgaggaaccc gccgtagccg ccgaccgcag tcagcgcgag gaccgcggcg atgctccgca
     2881 gcccgccgag cgcggcgatg cccacgccgg acacgacgaa gccggcgacg accaagccgg
     2941 gcttcgcgag ccgccgtccg atcgcggggg ccgtcaccgc tccgaggaat gcgccggccc
     3001 ctgcgctgcc gagcgcgagc gcgccggacg acaaccgtcc gaacctgtcg ccggcgtccg
     3061 ggaactcgct cgttatgacg aggatggcag cgatcgcgac gatcatgccg atcgtgcgca
     3121 ggacgaagat cgcggcgagc gggatccgtg cccggggccg tcgcaggatc tcgacgactc
     3181 cctcggccag ctcgcgcacg acccgccggg ccgccgcgcc gaaggtctcg tccggggcgt
     3241 gcgggtgcgc gaacgacctc gagatccgcc gcgtgatcca cgccgcgccc ccgtagaaga
     3301 gccccgcggc cgcgaacgag ggacgggggc cgagccagcc gactacgccg acgccgacca
     3361 cgccgccgag gagcgcggag atcatcccgc ctccactcga cagcgcgttg ccgcgcagca
     3421 gatggtgctc ctccagcacg acgggaagca cggccccctt cgtgacgagg aacagacgtc
     3481 ccagcccgag cacgacgagg acgccgacgt acagcgcggc gtcgtcgggc atcagcccgg
     3541 cccacaacgg cagcgtgacg agcacgaccg cgcgcgccgc gttcgtcgcg gccatcagct
     3601 cgcggcgtct ccaccggtcg acgaacacgc cgaggaacgg cgcgagcagc gaatacggga
     3661 ggagcgtgat cgcgaacgcg acgaggatcc gtcccggggt ctcgcccagg atcagcgctt
     3721 cggcgaacgc gacctgcgcg agcccgtcgg ccgcctgcgc ggtccattgc gcgctcagca
     3781 gcgtcctgag gtcgcgctgc gcgagcagct gccggacctt cgtccggccg gagaccgggg
     3841 cgctcaccgg cgccccggca ccagaggtgc gtctacgccc ggcgcttgag cgcggggtcg
     3901 gcgtcgagcg agaacgacgc gacgccgttc gagcctgccg ccccacccag gtcgccgcgg
     3961 atgaactcct cgacgagctc gtgggcctcg gagtccgtgt actggatcgg aggcgacttc
     4021 atgaagtacg cgctgggggc gagcagcggg ccgccgatgc cgcggtcgag ggcgagcttg
     4081 acgcagcgga cggcgtcgat cacgacaccc gcagagttgg gtgagtccca tacctccagc
     4141 ttcagctcga tgttcacggg gatgtccccg aactcgcgtc cctcgatacg aatgtaggcc
     4201 cacttgcggt ccttcagcca cgggacgtgg tccgacgggc cgatgtggat gtcctccttg
     4261 tcgatgccgt tctcgatctg cgacgtcacc gactgggtct tcgagatctt cttcgactgg
     4321 agccgcgaac gctcgagcat gttcatgaag tccatgttcc cgccgaagtt cagctggtag
     4381 gtgtggtcga tcgtcacgcc gcggttctcg aacagcttcg agagcacgcg gtggctgatc
     4441 gtggcgccga gctggctctt gatgtcgtcg ccgatgatcg gcacgccctt cttcacgaag
     4501 cgctgtgccc actcctcgtt cgtcgcgatg aacaccggca tgcagttgac gaagccgacg
     4561 ccggcctcga gcgcctgctc cgcgtagaag cgagcggcct gctcggagcc gaccggcagg
     4621 tacgacacga gcacgtcggt ctgcgtctcg cgcaggacgc gggcgacgtc gaccggcggg
     4681 agctcggact cggtgacgac ctcgcggtag tagtgcccga ggccgtcgta ggtgtggccc
     4741 cggaggacct cgatgcccgt ctgcggcacg tcggagaact tgatcgtgtt gttcggctcg
     4801 gcccagatgg cgtcggcgag atcgaggccg accttgttcg agtcgacgtc gaacgcgcac
     4861 gtgaactcga cgtcggagac gtggtacccg ccgagctgga cgtgcatgag cccgggcacg
     4921 aactcgtctt ccttcgcctt ggcgtagtac tcgatgccct ggacgagtga cgacgcgcag
     4981 ttcccgactc cgacgattcc gactcgtacc tttgccatgt gagctccccc ttcgttcccg
     5041 ttcccgggtg tcagatcccc gagggattcg cgcggccggc ctccagcgag cgctcctccc
     5101 tgatcaactc gtcgatccat gcgatgtcgt tctccgttgc agccatcccg tggttctgca
     5161 gcgagagcgt gtagctgtcg atccgctcgc ggtagtcgcg caggttggcc ttgacctgcg
     5221 cgagcttttc ctggaggtac gctcgccggc gctcgaggag cgcgagccgg gtctcgggct
     5281 ggaggtac
//
LOCUS       DASQDO010000159         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_811755, whole genome shotgun sequence.
ACCESSION   DASQDO010000159 DASQDO010000000
VERSION     DASQDO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811755"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="hflX"
                     /locus_tag="VEV43_04645"
     CDS             complement(<1..1077)
                     /gene="hflX"
                     /locus_tag="VEV43_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYP22840.1"
                     /translation="MHLSGSHATRPPREKAILVAAVFGEGSAEEAEWSLEELEALADT
                     AGADALDTVIQRRDNPHPATYIGKGKVQEVVATANALDADMLIFDDELTPAQGRNLEE
                     IAGVNPLAERGLKIIDRTGLILDIFAQHARSAEGKLQVELAQLNYRLPRLRGWGDVLS
                     RLGGGIGTRGPGETALEAERRAILRRIQRVKKDLDDLERTRLVKRKQRTRTGITVIAL
                     VGYTNAGKSTLLRRLTSADVLVQNQLFSTLDPTTRRLSLPGEREALLTDTVGFVRKLP
                     HQLVEAFRSTLEEVREASMLLHVVDASRDPERQIAAVDTVLREIDAAGKPTVLALNKS
                     DLLTEPERDKIRGRFPEAVLCSAAK"
     gene            complement(1068..>1899)
                     /locus_tag="VEV43_04650"
     CDS             complement(1068..>1899)
                     /locus_tag="VEV43_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYP22841.1"
                     /translation="AFVDPGDVALIPDPGYPVYETGTILSHGEPLLLPLTPENGYVPD
                     LDAIPAEKLERAKVLWLNYPSNPTAACVDVDVFQRAVDFCRANDLLLAHDAAYTEITF
                     DDYVAPSALEADGAEDCVVEFHSLSKTYNMTGWRIGWVAGAPVAIEAMKRLKTNVDSG
                     IFDAVQHAGIAAIEGPQDYLHECVAIYRRRRDLLCDGLKSMGVVVEPPRGSIYVWVPV
                     PDGHTSESFTELLLNEAAIVVAPGNGYGPSGQGFVRFSLTIADDRLEEGVERLRKAFT
                     "
BASE COUNT          257 a          667 c          662 g          313 t
ORIGIN      
        1 cttcgcggcc gagcacagca ccgcctcggg gaaccggccc cggatcttgt cccgttcggg
       61 ctcggtcaac aggtcgctct tgttcaatgc cagcaccgtc ggcttgccgg ccgcgtcgat
      121 ctcgcgcagc acggtgtcca cggccgcgat ctgtcgctcc ggatcccggc tcgcgtccac
      181 gacgtgcagg agcatgctcg cctcgcgcac ctcctcgagc gtcgagcgga acgcctcgac
      241 gagctggtgc gggagcttgc ggacgaagcc cacggtgtcg gtcagcagcg cctcgcgctc
      301 acccggcagc gacagcctgc gcgtggtcgg gtccagcgtc gagaacagct ggttctgcac
      361 gagcacgtcc gcagaggtca gccggcgcag cagcgtcgac ttgccggcgt tcgtgtagcc
      421 gacgagcgcg atgacggtga tgccggtgcg cgtccgctgc ttgcgcttga ccaggcgagt
      481 ccgttcgagg tcgtccaggt ccttcttcac gcgctggatc cgccgcagga tcgcgcggcg
      541 ttcggcctcg agcgcggtct cgcccggccc cctcgtcccg atgcctcctc cgaggcggga
      601 cagcacgtcg ccccagccgc gcaggcgcgg gaggcggtag ttgagctgcg cgagctcgac
      661 ctggagcttc ccctccgcgc tgcgcgcgtg ctgggcgaag atgtcgagga tcaaccccgt
      721 gcggtcgatg atcttcaggc cgcgctccgc cagcgggttc acgcctgcga tctcttcgag
      781 gttgcggcct tgcgccggcg tcagctcgtc gtcgaatatc agcatgtccg cgtcgagcgc
      841 gttggcggtc gcgacgacct cctgcacctt gcccttgccg atgtacgtcg cggggtgcgg
      901 gttgtcgcgc cgctggatca cggtgtcgag cgcgtcggcc cccgccgtgt cggcgagcgc
      961 ctcgagctcc tcgagcgacc actccgcctc ttcggccgag ccctcgccga agacggccgc
     1021 gacgaggatc gccttctcgc gtggtggccg ggtggcgtgc gaaccgctca ggtgaatgcc
     1081 ttccgcagcc gctcgacgcc ttcctcgagg cggtcgtccg cgatcgtcag cgagaaccgc
     1141 acgaagccct ggccggaggg gccgtagccg ttgcccgggg ccacgacgat cgcggcctcg
     1201 ttcagcagca gctccgtgaa gctctccgag gtgtggccgt cggggacagg cacccacacg
     1261 tagatcgagc cgcgcggggg ctcgacgacg acccccatcg acttcagccc gtcgcacagc
     1321 agatcgcgcc tgcgccggta gatcgcgacg cactcgtgca ggtagtcctg cgggccctcg
     1381 atcgcggcga tgccggcgtg ctggaccgcg tcgaagatgc ccgagtcgac gttcgtcttg
     1441 agccgcttca tcgcctcgat cgcaaccggg gcgccggcga cccagccgat ccgccagccg
     1501 gtcatgttgt aggtcttgct cagcgagtgg aactcgacga cgcagtcctc cgcaccgtcg
     1561 gcctcgagcg cgctcggcgc gacgtagtcg tcgaacgtga tctccgtgta cgcggcgtcg
     1621 tgcgcgagca agaggtcgtt ggcccggcag aagtcgacag cgcgctggaa cacgtcgacg
     1681 tccacgcacg cggcggtcgg gttcgacggg tagttgagcc acaagacctt ggcgcgctcg
     1741 agcttctcgg ccgggatcgc gtcgaggtcc ggcacgtatc cgttctcggg cgtcagtggg
     1801 agcagcagcg gctcgccgtg cgacaggatc gtgccggtct cgtagacggg atagccgggg
     1861 tcggggatca gcgcgacgtc acccgggtcg acgaacgcg
//
LOCUS       DASQDO010000160         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_200027, whole genome shotgun sequence.
ACCESSION   DASQDO010000160 DASQDO010000000
VERSION     DASQDO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200027"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VEV43_04655"
     CDS             <1..775
                     /locus_tag="VEV43_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYP22842.1"
                     /translation="VELFGVTSTFVVPTHLERIFSLEPKVLKRHYSSSMRMLIHAGAP
                     IREATKRRAIDFFPEGSVWEFYGSTEGGATRISPEEWLRKPGSVGTPRPDAAILIRDE
                     SGAPVPRGEVGVVWVRDPHAERFSYWGDEEKTRGAWDGDAFTVGDLGRLDEDGYLFLE
                     GRAHDTIISGGVNVYPQEVEAVLSEHPSVLECAVYGREDEEWGQEVCALVVAADGFDA
                     DEVRAWARERLASFKVPRHYEVVDELPRTATGKVLRRGP"
     gene            1265..1555
                     /locus_tag="VEV43_04660"
     CDS             1265..1555
                     /locus_tag="VEV43_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22843.1"
                     /translation="MAKARIDMGPTTALAFEALTTRRDSLIVARHTLREIAASPVLEG
                     AESFLHGARQLALIFGRDPREFLDRVPCHPERRAWMQSQRYLERTPDVPGPT"
     gene            complement(1685..1861)
                     /locus_tag="VEV43_04665"
     CDS             complement(1685..1861)
                     /locus_tag="VEV43_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22844.1"
                     /translation="MNRFATLAMALTAATVLVPAPPAGATGCVIDDIVEDLPRCVIYG
                     SPPVYQTCQKLGLC"
     gene            complement(1882..2064)
                     /locus_tag="VEV43_04670"
     CDS             complement(1882..2064)
                     /locus_tag="VEV43_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22845.1"
                     /translation="MRKRLASVVLPVTAAAVLLPAAPASATTCILLPDPDVGDVYCLV
                     FSEPAVQAVCAKLVVC"
     gene            complement(2138..2881)
                     /gene="trxA"
                     /locus_tag="VEV43_04675"
     CDS             complement(2138..2881)
                     /gene="trxA"
                     /locus_tag="VEV43_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYP22846.1"
                     /translation="MSAYEVTDATFEDDVLRKSFQQTVVVDFWAEWCGPCRQLTPVLE
                     RLADESPDWVLAKVDVDANPRLSATFGIQSIPAVKAFRDGKQVGEFMGALPESQVRKW
                     IEQLGPSEGELALVVAEEAEADGRLAEARSGYERALQEEPGLFAARSGLARIDLIMRV
                     QNLDEATLKGRAETNPSDLDAAVGLADLDAAAGRHAQAFGRLIEAIRTSSDDDRERLR
                     KHLLGLFDTLPADDPRVLSSRRDLSMALF"
     gene            complement(2886..3527)
                     /locus_tag="VEV43_04680"
     CDS             complement(2886..3527)
                     /locus_tag="VEV43_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYP22847.1"
                     /translation="MSLDGFVAGPNPSVEEPLGEGGEALHEWAFALEAWRRPHGLEGG
                     DVNASTAVMEDALSNAGATIMGRKMFGGGPGPWGDEPWDGWWGDDPPFHHPVFVLTTH
                     PRGPLAKGDTAFTFVTDGIESALDQARAAANDGDVRLAGGADVAQQYLSAGLLDEVQI
                     DLVPILLHGGTRLFEGLAPGIRFEQVQAIEAPGVTHLKYRVTYGEGSDGSGRS"
     gene            complement(3560..4507)
                     /gene="mdh"
                     /locus_tag="VEV43_04685"
     CDS             complement(3560..4507)
                     /gene="mdh"
                     /locus_tag="VEV43_04685"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018791947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HYP22848.1"
                     /translation="MAGATKKVTVVGAGNVGSNTARRIAERNLADEVVMIDIVEGLPQ
                     GLALDINQSAPTEGFETLVTGTNDYADTAGSDVVVITAGLPRKPGMSRMDLLDANSEI
                     VGGVTKQIAEHSPDTVIIVVSNPLDEMTYLAALVSEFPKERVMGMAGVLDSSRLRFFI
                     AEKLNVAPTSVEAITLGSHGDQMVALPRHATVEGKPLPELVDEPALQELFTRTRDAGA
                     EIVGYLKKGSAFYAPSSSSATMVESILRDSDDVHPTCAWVSGEYGISDTYLGVPAKLG
                     AGGVTGVVELELNDDETSQLEEAANAIRAKCEELAARRK"
     gene            complement(4558..5655)
                     /locus_tag="VEV43_04690"
     CDS             complement(4558..5655)
                     /locus_tag="VEV43_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HYP22849.1"
                     /translation="MSSKVHRVTLIPGDGTGPEISEATRRVLEATGVSFDWDVQHAGV
                     DVMDEYGTPLPEKVLDSIRENKVAIKGPITTPVGTGFRSVNVALRKELDLYMCLRPCK
                     SYKGVRSRYEDIDLVVVRENHEDLYAGIEFEQGSPDARKLIDFMADLGARRVREDSGI
                     SIKPISVTGTRRIVKAAFDYAIKHGRKKVTAVHKANIMKFTDGLFLKTAREVGEEYAD
                     SGVEFEDRIVDNMCMQLVQKPELYDVLVLPNLYGDIVSDLGAGLVGGLGVAPGGNIGD
                     EAAVFEPTHGSAPKYAGQNKVNPMAMMLSGVLMLDYLEETDAAKRLEAAISAVIEEGE
                     SVTYDMKPSRDDPSAVGTSEVADAIIEKLNA"
BASE COUNT          928 a         1970 c         1979 g          968 t
ORIGIN      
        1 ggtcgagctc ttcggcgtca cctcgacgtt cgtcgtgccg acgcacctcg agcggatctt
       61 ctcgctcgag ccgaaggtgc tgaagcgcca ctactcgtcg agcatgcgga tgctgatcca
      121 cgcgggggcc ccgatccgcg aggcgacgaa gcggcgcgcg atcgacttct tccccgaggg
      181 ttcggtgtgg gagttctacg ggtcgacgga gggaggcgcg acgcggatct caccggagga
      241 gtggctgcgc aagcccggga gcgtcggcac gccccggccg gacgcggcga tcctgatccg
      301 ggacgagtcg ggtgcgcccg tgccgcgggg cgaggtcggc gtggtctggg tgcgcgaccc
      361 ccatgccgag cggttctcgt actgggggga cgaggagaag acccggggcg cgtgggacgg
      421 cgacgcgttc acggtcggcg acctcgggcg gctggacgaa gacggttatc tgttcctgga
      481 gggccgcgcc cacgacacga tcatcagcgg gggcgtgaac gtgtacccgc aggaggtcga
      541 ggcggtgctg tcggagcacc cgtcggtgct ggaatgtgcg gtgtacgggc gggaggacga
      601 ggagtggggg caggaggtct gcgcgctggt ggtcgcggcc gacggcttcg acgcggacga
      661 ggtgcgcgcc tgggcacgcg aacgtctggc gagcttcaag gtgccgcgtc actacgaggt
      721 ggtggacgag ctgccgagga cggcgacggg caaggtgctg cgccgggggc cgtgacgcgt
      781 gttaggaacg atcgtccttg acgcatacag atcggatgag cggcatgtgg ttgcagccgc
      841 tctggacgag ctctgctccc cgggcgatct gtacgggtgg gcatccacag gggtttattg
      901 cttttggaac agaaccgaga gaacgattct ttacatcggc ctctctgttg acctcccgct
      961 tcgttttcgc gagcacaacg ggttgcgaaa gactcgtgca agagggaaca aagcacgtca
     1021 ggtcgacgcg tacttccacg aagctgagta tttaggtttc tccgtcttcc ttcagtcgcc
     1081 catggcgcaa tcactttcag ccagggcctt ggtcccgttc ggcgtcgaag gccatcgcgt
     1141 ccgggagctg tacggccaca gcgtggccca cgggacggaa gcaatccgcc gggctgaagg
     1201 aatctggctc gaagcccatc gtcaaaccta cgggcacctg ccgccctgga atcgaatcgg
     1261 gggtatggcg aaggcgagga ttgacatggg acccacgacc gccctggcgt tcgaagccct
     1321 cactacgcgt cgcgacagct tgatcgtggc tcgccacact ctccgagaga tcgcagcaag
     1381 cccggtgttg gagggcgccg agtctttcct ccatggcgcc cgccagctcg cgttgatctt
     1441 cggtcgcgac ccccgcgagt ttctcgaccg ggtgccttgt catcccgagc gacgggcgtg
     1501 gatgcagtca cagaggtacc tcgagcggac cccggacgta cccggcccca catgaaggca
     1561 tggcgattct gcagtcccgg tcgaggtcta cgtcggagaa ccacgccgcg tccagaggca
     1621 cgagcctcaa ggcgaaaggc gcgccctgac cgagtccgga cggacctctc cttcgagaag
     1681 gtcgctagca gagaccgagc ttctgacagg tctggtagac gggaggcgac ccgtagatca
     1741 cgcagcgggg caggtcttcg acgatgtcgt cgatgacgca gccggtcgcg cccgccggag
     1801 gggccggcac cagcacggtc gctgccgtaa gggccattgc caacgtcgca aaacgattca
     1861 tcgaacgatc ctcctcgaat gtcagcagac gacgagcttg gcgcacacgg cctgcaccgc
     1921 aggctcgctg aagaccaggc agtaaacgtc gccgacgtcc gggtccggca agaggatgca
     1981 ggtcgtggcc gacgccggtg cagcgggcag caacacggcg gccgcggtca ccggcaacac
     2041 gacactggcg agacgcttgc gcaaaccgtc ctccttcgtc cgggagcccc aacggcccca
     2101 ccggcgaagg acttccacag ccggagatcg atctcctcta gaacagggcc atcgagaggt
     2161 cgcggcgcga cgacagcacc cgggggtcgt cggcggggag cgtgtcgaac aggcccagca
     2221 ggtgcttcct cagtcgctcc cggtcgtcgt ccgacgaggt gcggatcgcc tctatcaggc
     2281 ggccgaaggc ttgtgcgtga cggcccgccg ctgcgtccag gtcggccagg cccaccgccg
     2341 cgtcgaggtc cgacgggttc gtctcggccc ggcccttcag cgtcgcctcg tccaggttct
     2401 gcacccgcat gatcaggtcg atcctcgcca ggccgctgcg ggccgcgaac aggccgggct
     2461 cctcctgcag cgcgcgctcg tagcccgaac gggcctcggc gagcctcccg tcggcctcgg
     2521 cctcctcggc cacgaccagt gccagctcgc cctcggacgg gcccagctgc tcgatccatt
     2581 tgcggacctg cgactcgggc agcgcgccca tgaactcgcc gacctgcttg ccgtcgcgga
     2641 acgccttcac cgccgggatg ctctggatgc cgaacgtcgc cgacagacgc gggttcgcgt
     2701 cgacgtcgac cttcgcgagg acccagtccg gcgactcgtc cgccagccgc tcgaggaccg
     2761 gcgtcagctg acggcagggc ccgcaccact cggcccagaa gtcgacgacc acggtctgtt
     2821 ggaacgactt cctgagtacg tcgtcttcga acgtcgcgtc ggtgacctcg tatgcgctca
     2881 tgtattcagg aacgcccgga gccgtcggag ccttccccgt acgtcacgcg gtacttcagg
     2941 tgcgtcacgc cgggggcctc gatcgcctgc acctgttcga aacggatgcc cggagcgagg
     3001 ccctcgaaca agcgggtccc tccgtggagg aggatcggta cgagatcgat ctggacctcg
     3061 tcgagcagcc ctgcggagag atactgctgc gcgacgtctg cgccgcccgc gagccggacg
     3121 tccccgtcgt tcgcggcagc ccgggcctgg tcgagcgccg actcgatccc gtcggtgacg
     3181 aacgtgaacg ccgtgtcgcc cttcgcgagc gggccgcgtg ggtgagtcgt cagcacgaag
     3241 accggatggt gaaacggcgg gtcgtcgccc caccagccgt cccagggctc gtccccccag
     3301 gggcccgggc cacccccgaa catcttccgc cccatgatcg tcgcgccggc gttcgagagc
     3361 gcgtcctcca tgaccgcggt gctcgcgttg acatcgccgc cttccagccc gtgcggccgg
     3421 cgccaggcct cgagcgcgaa cgcccactcg tgcagcgcct cgccgccttc gccgagcggc
     3481 tcttcgacgc tcgggttcgg ccccgcgacg aagccgtcga gcgacatggc gatcgcgaac
     3541 ttgaagtcgc ccgctgccgt cacttgcggc gcgccgcgag ctcctcgcac ttcgctcgga
     3601 tcgcattggc ggcctcttcg agctgcgacg tctcgtcgtc gttgagctcg agctcgacga
     3661 cgcccgtcac gccaccggcc cctagcttgg cgggcacgcc gaggtaggtg tcggagatgc
     3721 cgtactcgcc gctcacccac gcgcacgtcg ggtggacgtc gtccgagtcc cgcaggatcg
     3781 actcgaccat cgtcgccgac gacgaggacg gcgcgtagaa cgcgctcccc ttcttcaggt
     3841 agccgacgat ctcggccccc gcgtcgcgcg tccgcgtgaa gagctcctgc aacgcgggct
     3901 cgtcgacgag ctcgggcagc ggcttgccct cgaccgtcgc atgccgcggg agcgcgacca
     3961 tctggtcgcc gtgggagccc aacgtgatcg cctcgaccga ggtaggcgcg acgttcagct
     4021 tctcggcgat gaagaagcgc agccgggagg agtcgagcac accggccatc cccatcacgc
     4081 gctccttcgg gaactccgag accagcgccg cgaggtaagt catctcgtcg agcgggttcg
     4141 agacgacgat gatcacggtg tccggcgaat gctcggcgat ctgcttcgtg acgccgccga
     4201 cgatctcgct gttcgcgtcg agcaggtcca tgcggctcat gcccggcttg cgcgggagac
     4261 ctgccgtgat cacgaccacg tcggagccgg ccgtgtcggc gtagtcgttc gtcccggtga
     4321 cgagggtctc gaacccctcc gtgggggccg actggttgat gtccagcgcg aggccctgcg
     4381 gcagtccctc gacgatgtcg atcatcacga cctcgtcggc gaggttgcgc tcggcgatcc
     4441 ttcgagcggt gttcgagccg acgttgcccg cgccgacgac ggttaccttt ttcgttgccc
     4501 cagccacgtt gccgtgcctc cttctagttc gtggccgatc gccgaggcga tcggtcgcta
     4561 agcgttcagc ttctcgatga tcgcgtcggc gacctcggac gtacccaccg ccgaagggtc
     4621 gtcgcgcgac ggcttcatgt cgtaggtgac ggactcgccc tcctcgatca ccgccgagat
     4681 cgcggcctcc agccgcttcg ccgcgtcggt ctcctcgagg tagtcgagca tcagcacgcc
     4741 cgagagcatc atcgccatcg ggttgacctt gttctggccc gcgtacttgg gggccgagcc
     4801 gtgcgtgggc tcgaagaccg ccgcctcgtc accgatgttc ccgccgggcg cgacgcccag
     4861 gcccccgacg agccccgcgc cgaggtcgga cacgatgtcg ccgtagaggt tcggcaggac
     4921 gaggacgtcg tagagctcgg gcttctgcac gagctgcatg cacatgttgt cgacgatgcg
     4981 gtcctcgaac tcgacgcccg aatccgcgta ctcctcgccg acctcccggg cggtcttgag
     5041 gaacagcccg tcggtgaact tcatgatgtt ggccttgtgc acggccgtga ccttcttgcg
     5101 cccgtgcttg atcgcgtagt cgaacgccgc cttcacgatc cggcgcgtgc cggtgaccga
     5161 gatcggcttg atcgagatgc cggagtcctc gcggacccgg cgcgccccga ggtcggccat
     5221 gaagtcgatg agcttgcgtg cgtccggcga cccctgctcg aactcgatgc cggcgtagag
     5281 gtcctcgtgg ttctcacgca cgacgacgag gtcgatgtcc tcgtagcgcg accgcacgcc
     5341 cttgtaggac ttgcagggac gcaggcacat gtagaggtcg agctccttgc gcagcgcgac
     5401 gttgacgctg cggaagcccg tacccaccgg cgtcgtgatc ggtcccttga tcgcgacctt
     5461 gttctcgcgg atcgagtcga ggaccttctc gggcagaggc gtgccgtact cgtccatcac
     5521 gtcgacgccc gcatgctgga cgtcccagtc gaacgagacg ccggtggctt cgagcacgcg
     5581 ccgggtggcc tcggagattt cgggaccggt cccgtcgccg gggatcagcg ttacgcgatg
     5641 aactttggat gacattggaa ctcccgtcgt aaaggggtca ccgaccagga aaaagtgggg
     5701 ataaacctac ctgatagcct tctgacggag agatgaggaa gcgcctgtga gcgcccggag
     5761 agaaaggcca cgccccggat ggcagcaccc ggcaagggac tcgaaggtgt cgtagcggcc
     5821 gagaccgcgc tcagcgacat cgacg
//
LOCUS       DASQDO010000161         8391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_751141, whole genome shotgun sequence.
ACCESSION   DASQDO010000161 DASQDO010000000
VERSION     DASQDO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751141"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VEV43_04695"
     CDS             <1..754
                     /locus_tag="VEV43_04695"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYP22850.1"
                     /translation="AFQFSALKTVDPSPFDLAGRTLESVGRRGKYLVFSFGGLNVLVH
                     LSQGGRFDFEDPPKSTKPRGSVARFVFESGPSLLVKEFGTERKAAWWVLRPDDPGPLQ
                     GLGPEPFDDAFDAIVLETSDGRQLHNWLRDQKVVAGIGRGFADDVLHEAMLSPVKKTA
                     SMTAEERERLLGAVRKVLTEATDRERARTGGLPAKMGDRFTIHNHYGQPCPRCRDALR
                     HVVYESREVVYCPRCQTGGKVLADRVLSQFLK"
     gene            complement(747..1061)
                     /locus_tag="VEV43_04700"
     CDS             complement(747..1061)
                     /locus_tag="VEV43_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22851.1"
                     /translation="MATRRHEDAHDVMRMLEGHLPAMTEILEHTRPAPTAGATTPDGR
                     KIKECVAVMLVEEPDGSETVMIVGDAEAEHLALKGILHDGIYAFAHKGEPGFGLPSQT
                     TT"
     gene            complement(1161..2522)
                     /gene="lysA"
                     /locus_tag="VEV43_04705"
     CDS             complement(1161..2522)
                     /gene="lysA"
                     /locus_tag="VEV43_04705"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HYP22852.1"
                     /translation="MRPAPHREPRKAALNRALLQVFPDTASVAPSGHLSVGGCDVTQL
                     AEEHGTPVIVFDRATLEARAAAYTREVPPANVLYAGKAFCCIAVCEILRDLGLGLDVC
                     TGGELATALAAGFPPERIVFHGNNKSPEELVMARDAGVGRIVADSFDELHRLQDVGWP
                     HKVLIRVTPGVEAHTHEYVQTGHEDSKFGFTLADGIALSALRTAAGLSLDLAGLHAHI
                     GSQIFEEDAFELAAARLAGLAGEATEKLGLVMRELDLGGGLGIAYTPDDDPPDPAELV
                     AAITRVARSAFAERGLAVPEILFEPGRALAGPAGVTVYRVGTVKRIPRARGHADQPDR
                     VYVSVDGGMSDNPRHALYGAVYEAALANRMGDEAALWCSVAGKHCESGDVLIRDVRLP
                     ADVAPGDLLCIPATGAYTYSMASNYNRLPRPPVVLVHEGEASVIVRRESYEDVLRLDV
                     PLG"
     gene            complement(2480..3346)
                     /locus_tag="VEV43_04710"
     CDS             complement(2480..3346)
                     /locus_tag="VEV43_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HYP22853.1"
                     /translation="MDFLTVSVFHAGDPFRGGNPLAVFPEAGGLEPGQMQAIAQTLNL
                     SETTFVTGGDSSSYDVRIFTPGDELPFAGHPTLGTAWVLRHLGRLTADAVVQRSPAGE
                     TKVSFEGETLSFERTGKAWPDLEATNERAHLDVARALRIDERDVTLEGYELGRPTVLR
                     PALSDAGLQHFVVPLRDVDALERVDVDGARLAELSPFGAYCFTGVGAGKVRARGLFPA
                     VGVDEDAATGSAAACLGVYLADRVGDIELTIDQGVEMGRPSRMTVSATADGVRVGGRC
                     DLLLTGNLERLP"
     gene            complement(3364..5007)
                     /gene="argS"
                     /locus_tag="VEV43_04715"
     CDS             complement(3364..5007)
                     /gene="argS"
                     /locus_tag="VEV43_04715"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYP22854.1"
                     /translation="MIADRLADLLRAALADAGAATATPIHFERPRRREHGDWSTNVAL
                     VAAKEQGKNPRELAATIVEHLPESALVEKVEVAGPGFLNFHLSAEWLHDVVRRAADPA
                     ARFGRSDYGKGKKVNVEFVSANPTGPINVVSGRHAAVGDTIASLLEATGHQVTREFYV
                     NDAGRQAQLFGESIATHYLRHFGVEAELPEDGYKGDYVADLARDLAGELGPRLLDASP
                     EERSAALRDLGLLRMQQEMRISLERFRTRYDVWFSERTLHESGSIDGTLGYLKEQGLA
                     EERDGALWFLSTKFGDDKDRVLVRDNGVPTYLAGDAAYLRDKFGRGFEHLIYLWGADH
                     HGTVARLKAMAQALGHDREGVEIRLVQVVTLKNEGQTLKGSKRAGVFVSLDELVEQVG
                     VDAARYTFLTRGIDAPLDFDIEAAKEQAPENPVYYVQYAHARICSILRKAAESGHTAD
                     VPSAPLDRLGHPSEDAVMRKLASFEEVVVAAAEARSPQKITRYVEELASQFSGFYRDC
                     KVVTDDSDLTAARLALCVATKCVIAEALGLLSVDAPDRM"
     gene            complement(5012..5386)
                     /locus_tag="VEV43_04720"
     CDS             complement(5012..5386)
                     /locus_tag="VEV43_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP22855.1"
                     /translation="MADPQKRVLICDDDPVILRLLEVNLELEGYDVLTAHHGEEAFEI
                     ASRELPDLVILDIMMPRLDGYQTCEKLKAQPSTEPIPVVFLSAKAQQSDIEKGKSYGV
                     SEYLTKPFDPNDLLDVVERLVG"
     gene            5526..5600
                     /locus_tag="VEV43_04725"
     tRNA            5526..5600
                     /locus_tag="VEV43_04725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5559..5561,aa:Arg,seq:ccg)
     gene            complement(5710..6372)
                     /locus_tag="VEV43_04730"
     CDS             complement(5710..6372)
                     /locus_tag="VEV43_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYP22856.1"
                     /translation="MAKRRDQDVLVAGLGRFGTSVAETLLSLGRDVLGVDADAEVVQE
                     HAGRFAHVVEADTTDEEALRQLGAAEFRRAVVGIGTDVEASFLTTAALVDLGVEEVWA
                     KAVTTAHGRILERVGAHCVVYPERESGERIAHRVTGRMIDYIGLDENFALVETRAPRE
                     LAGKSLRDSEVRRRFGITVVCIKSEGQAFTYATAETVVRPGDILVVAGERKRAEEFAE
                     RD"
     gene            complement(6365..7759)
                     /locus_tag="VEV43_04735"
     CDS             complement(6365..7759)
                     /locus_tag="VEV43_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HYP22857.1"
                     /translation="MPSHAASGRTVAGGGASRRLLARFRLRHPSQFVVAAVFAAIVVG
                     TILLSLPVATEGPAAASFTTAMFTATSAVCVTGLAVVDTGAYWSGFGEGTILVLIQLG
                     GFGFMTLASMLALVVYRNLGVRARLLAQAETKTLDLGDVRRIVVGVAVVSFLFEAVSA
                     VVLALRFGTAYGEPAGRALYLGVFHAVSAFNNAGFSVFAGNLKEFVGDAWIICVIAVG
                     LIVGGIGFPVLFEVKRSWRRPSHWSLHTKLTLVMTGALIVAGTAAVLAFEWGNSATLG
                     EMSVPGKGLAGFFQGVSPRTAGFDAVDIGALTEPTLLVTMVLMFIGGGSAGTAGGVKV
                     TTVALLGAMVRAEVRGEPAVELMDKRVPGAAQRQALAVVLAGFFAVFTGTLVLMAAEG
                     IAMKGALFEAFSAFGTVGLTTGVTPDLSSVGRLVLVVLMFVGRVGPITFGAALAMREH
                     RRRFRYPEERPIVG"
     gene            complement(8109..8315)
                     /locus_tag="VEV43_04740"
     CDS             complement(8109..8315)
                     /locus_tag="VEV43_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22858.1"
                     /translation="MKRSVKALLVSGVMAASVWGTAAPAGAATCVGVEFRNGFQNAGA
                     WTCTCPSDPKHGPIDGQFWLILCV"
BASE COUNT         1286 a         2930 c         2921 g         1254 t
ORIGIN      
        1 cgcgttccag ttctcggccc tcaagaccgt ggacccgtcc ccgttcgacc tcgccgggcg
       61 cacgctggag agcgtcgggc gccgcggcaa gtacctcgtc ttttcgttcg ggggcttgaa
      121 cgtcttggtg cacctctcgc agggagggcg gttcgacttc gaggacccgc cgaagtcgac
      181 gaagccgcgc ggctcggtgg cgcgcttcgt cttcgagtcc gggccgtcgt tgctcgtgaa
      241 ggagttcggc accgagcgga aggcggcatg gtgggtgctg cgcccggacg atcccggccc
      301 gctccagggc ctgggcccgg agccgttcga cgacgccttc gacgcgatcg tgctcgagac
      361 gagcgacggg cggcagctgc acaactggct gcgcgaccag aaggtcgtcg ccggcatcgg
      421 ccgcggcttc gcggacgacg tgctgcacga ggcgatgctg tcgccggtga agaagacggc
      481 gtcgatgacg gccgaggagc gggaacggct gctcggcgcg gtgcgcaagg ttctgacgga
      541 ggcgaccgac cgcgagcggg cgcggaccgg agggctcccg gcgaagatgg gcgaccgctt
      601 cacgatccac aaccactacg gacagccgtg cccgcgctgc cgcgacgcgc tacggcacgt
      661 cgtgtacgag tcgcgtgagg tcgtctactg cccgcgctgt cagaccggcg gcaaggtcct
      721 tgcagaccgc gtgctgtcgc agttcctcaa gtagtcgtct gcgacgggag gccgaacccc
      781 ggctctccct tgtgggcgaa cgcgtagatg ccgtcgtgga ggatgccctt gagcgcgagg
      841 tgctcggcct cggcgtcccc gacgatcatc acggtctcgg aaccgtcggg ctcctcgacg
      901 agcatcaccg cgacgcactc cttgatcttg cgaccgtccg gggtcgtcgc gccggcggtt
      961 ggcgccggcc tggtgtgctc gaggatctcg gtcatcgcgg ggaggtgccc ctccagcatc
     1021 cgcatcacgt cgtgcgcgtc ctcgtgccgc cgcgtcgcca tctgtgtcgc tccctccgtc
     1081 gagtccgccc gtccgaaaga tgaccgtgtg cgccattcgc gacaaggggg aacaccgata
     1141 taggcggatt gtccggaaga tcagccgagc gggacgtcga ggcgcagcac gtcttcgtac
     1201 gactcgcgcc ggacgatcac gctcgcctcg ccttcgtgga cgagcaccac cgggggccgc
     1261 ggcagacggt tgtagttcga cgccatcgag taggtgtacg cgcccgtcgc ggggatgcac
     1321 agcaggtcgc cgggcgcgac gtcggcggga aggcgcacgt cccggatcaa gacgtcgccg
     1381 gactcgcagt gcttcccggc gacggagcac cacagcgccg cctcgtcgcc catccggttc
     1441 gccagcgcgg cctcgtagac cgctccgtag agagcgtggc gagggttgtc gctcatgccc
     1501 ccgtcgaccg agacgtagac gcggtccggc tggtcggcgt ggccgcgcgc ccgcgggatc
     1561 cgcttgacgg tgccgacgcg gtagacggtg acgcccgccg gtccggcgag cgcccggccg
     1621 ggctcgaaga ggatctcggg gaccgcgaga ccgcgctcgg caaacgcgct gcgcgcgacc
     1681 cgggtgatcg cggcgacgag ctcggccggg tcgggagggt cgtcgtcggg cgtgtaggcg
     1741 atcccgagcc ctccgccgag gtcgagctcg cgcatcacga gacccagctt ctccgtcgcc
     1801 tcgccggcga gccccgcgag gcgcgccgcg gcgagctcga acgcgtcctc ctcgaagatc
     1861 tgcgacccga tgtgggcgtg gagccccgcg aggtcgagcg agagcccggc ggcggttcgc
     1921 agcgcgctca gcgcgatccc gtccgccagc gtgaacccga acttcgagtc ctcgtggccg
     1981 gtctgcacgt actcgtgggt gtgcgcctcg acgccgggcg tcactctgat caggaccttg
     2041 tggggccatc cgacgtcctg gagccggtgg agctcgtcga acgagtcggc gacgatcctg
     2101 cccacgccgg cgtccctcgc catcacgagc tcttccggcg acttgttgtt gccgtggaag
     2161 acgatgcgct ccggcgggaa gcccgcagcc agcgcggtcg cgagctcacc gcccgtgcag
     2221 acgtcgaggc cgagccccag gtcgcgcagg atctcgcaca ccgcgatgca gcagaacgcc
     2281 ttgcccgcgt agagcacgtt cgcgggcggg acctcgcgtg tgtatgccgc ggcccgcgcc
     2341 tccagcgtcg cgcggtcgaa gacgatcacc ggcgtgccgt gctcctccgc gagctgcgtc
     2401 acgtcacacc cgccgaccga cagatgaccg gacggcgcga ccgacgcggt gtcggggaag
     2461 acctgcagca gcgcgcggtt cagggcagcc tttcgaggtt cccggtgagg agcaggtcgc
     2521 agcggcctcc gacgcggacg ccgtcggcgg tcgccgagac ggtcatgcgc gacggcctgc
     2581 ccatctcgac gccctggtcg atcgtcagct cgatgtcgcc gacccgatcg gccaggtaga
     2641 cgccgagaca tgccgcggcc gatccggtcg ccgcgtcctc gtcgacgccg accgccggga
     2701 agagcccgcg ggcccgcacc ttgccggccc ccacgcccgt gaagcagtag gcgccgaagg
     2761 ggctcagctc cgcgagccgg gccccgtcga cgtcgacgcg ttcgagggcg tcgacgtcgc
     2821 gcagcggtac gacgaagtgc tgcaggcccg cgtccgagag tgcggggcgc aggacggtgg
     2881 gacgcccgag ctcgtatccc tccagcgtga cgtcgcgctc gtcgatccgc agagcgcgtg
     2941 cgacgtcgag gtgcgcgcgc tcgttcgtcg cctccaggtc aggccacgcc ttccccgtcc
     3001 tctcgaacga cagcgtctct ccttcgaacg agaccttcgt ctcaccggcg ggcgaccgct
     3061 gcacgactgc gtccgcggtg aggcggccga ggtgacggag gacccacgcg gtgccgagcg
     3121 tcgggtggcc ggcgaacggg agctcgtctc cgggcgtgaa gatccgcacg tcgtaagagg
     3181 acgagtcgcc gccggtgacg aacgtcgtct cggagaggtt gagggtctgg gcgatcgcct
     3241 gcatctgccc gggctcgagc ccgccggcct cggggaagac ggccaggggg ttcccgccgc
     3301 ggaacgggtc gcccgcgtgg aagaccgaga ccgtgaggaa gtccatcggc ccagtatcgc
     3361 ggctcacatg cgatcggggg cgtcgacgct cagcaggccc agggcctcgg cgatgacgca
     3421 cttggtcgcg acgcacagcg caagccgggc cgcggtgagg tcggagtcat cggtcactac
     3481 cttgcagtcc cggtagaacc cggagaactg cgacgcgagc tcctcgacgt agcgcgtgat
     3541 cttctgcggc gagcgggctt cggccgcggc gacgacgacc tcctcgaacg acgcgagctt
     3601 gcgcatcacc gcgtcctcgc tcgggtggcc gagccggtcc agcggcgcgc tcgggacgtc
     3661 ggcggtgtgc ccggactcgg ccgccttgcg caggatcgag cagatgcgcg cgtgcgcgta
     3721 ctggacgtaa tagacggggt tctcgggagc ttgctccttg gccgcttcga tgtcgaagtc
     3781 gagcggggcg tcgatgcccc gcgtcaggaa cgtgtagcgg gccgcgtcga cgccgacctg
     3841 ctcgacgagc tcgtcgagcg agacgaacac cccggccctc ttcgagccct tgagcgtctg
     3901 gccctcgttc ttcagcgtga cgacctggac gagccggatc tcgacgcctt cgcgatcgtg
     3961 tccgagcgcc tgcgccatgg ccttcaggcg cgcgacggtc ccgtggtggt cggctcccca
     4021 gagatagatc aggtgctcga agccgcggcc gaacttgtcg cggagatatg ccgcgtcgcc
     4081 ggcgaggtag gtcgggacgc cgttgtcacg aacgagcacg cggtccttgt cgtcgccgaa
     4141 tttcgtcgag aggaaccaga gcgcgccgtc gcgctcctcc gcgaggccct gctccttcag
     4201 gtagccgagc gtgccgtcga tgctgcccga ctcgtgcagc gtcctctccg aaaaccacac
     4261 gtcgtagcgg gtgcggaagc gttcgagcga gatccgcatc tcttgctgca tccgaaggag
     4321 gccgaggtcg cgcagggccg cgctgcgttc ttcgggactt gcgtcgagga gccgggggcc
     4381 gagctcgcca gccaggtcgc gcgcgaggtc ggccacgtag tcacccttgt atccgtcctc
     4441 gggcagctcc gcctcgacgc cgaagtggcg caggtagtgc gtcgcgatcg actctccgaa
     4501 caactgggcc tgccgtcctg cgtcgttgac gtagaactcg cgcgtgacct ggtgcccggt
     4561 cgcctcgagc aggcttgcga tcgtgtcccc gacggcggcg tgacgtccgc tgacgacgtt
     4621 gatcggcccg gtcgggttcg ccgacacgaa ctcgacgttg accttcttgc ccttgccgta
     4681 gtcgctgcgg ccgaaccgtg ctgcggggtc ggcggcccgg cgtacgacgt cgtggagcca
     4741 ctcggcggag aggtggaagt tcaggaagcc gggccctgcg acctcgacct tctcgacgag
     4801 tgcggactcg ggcaggtgct cgacgatcgt ggcggcgagc tcgcgggggt tcttgccctg
     4861 ctccttcgcc gcgaccaacg cgacgttcgt cgaccagtca ccgtgctcgc gccggcgggg
     4921 ccgctcgaag tggatcggcg tcgccgttgc agcgccggcg tccgccagtg ccgcccgcag
     4981 gaggtcggcg agccggtcgg cgatcaaggg ctcagcccac cagccgctcg acgacgtcca
     5041 agaggtcgtt gggatcgaac ggcttcgtca gatattcgga gacgccgtag ctcttgccct
     5101 tctcgatgtc ggactgctgc gccttcgcgg agaggaacac gacggggatg ggctcggtcg
     5161 agggctgggc cttgagcttc tcgcaggtct gatagccgtc gagcctcggc atcatgatgt
     5221 cgaggatgac gaggtcgggg agctcgcggc tggcgatctc gaacgcctcc tcgccgtgat
     5281 gggcggtgag gacgtcgtag ccctcgagct cgaggttgac ctcgaggagc cgcaagatga
     5341 cggggtcgtc gtcgcagatg aggacgcgtt tctgtgggtc ggccatggga cgagggagcc
     5401 taaagcaaga aaacttgcac ccttcttccc aggtcaggca aggtttccgc gccgtaaatg
     5461 caagattcgt ggcgcatcca agggcgccag caagatttct tgcatgctct agggtgacga
     5521 gccgggcccc cgtagctcag gggatagagc actcgcctcc ggagcgaggt gcgcaggttc
     5581 gaatcctgcc gggggcacca acacatctcg tcttggaacg ccgcgccgtg gccggtgcgc
     5641 ctcgcctact acgccgccat cagcgaccgc tggtaatgcg agacgagggg gccgcgacct
     5701 ccgcgcccct cagtcgcgtt cggcgaactc ctcggcccgt ttgcgctccc ccgccaccac
     5761 gaggatgtcg ccgggccgta cgacggtctc ggccgtcgcg taggtgaagg cctgaccttc
     5821 ggacttgatg cacacgacgg tgatgccgaa ccggcggcgg acctcgctgt cgcgcagcga
     5881 cttgcccgcc agctcgcggg gggcgcgcgt ctcgaccagc gcgaagttct cgtccaggcc
     5941 tatgtagtcg atcatccggc ccgtgacgcg gtgggctatg cgctcgcccg actcccgctc
     6001 ggggtagacc acgcagtggg ccccgacgcg ctcgaggatc cggccatgcg cggtcgtcac
     6061 ggccttggcc cagacctcct cgacgccgag gtcgacgagc gcggcagtcg tgaggaagct
     6121 cgcctccacg tccgttccga tcccgacgac ggcgcggcgg aactcggccg cgccgagctg
     6181 tctcagcgct tcctcgtcgg tggtgtccgc ctcgacgacg tgcgcgaacc ggccggcgtg
     6241 ctcctggacg acctctgcgt ccgcgtccac gccgaggacg tcgcgcccga gggacagaag
     6301 cgtctcggcg acgctcgtcc cgaaccggcc gagccccgcg acgaggacgt cttgatccct
     6361 tcgcttagcc aacgatcggt cgctcctccg gatagcggaa acggcggcgg tgctccctca
     6421 tcgccagcgc cgctccgaac gtgatcgggc cgacgcggcc gacgaacatg aggaccacca
     6481 acacgaggcg tcccaccgag ctgaggtcgg gggtgacccc cgtggtcagc cccaccgtac
     6541 cgaaggccga gaacgcctcg aacaaagcgc ccttcatcgc gatcccctcg gccgccatga
     6601 ggacgagcgt ccccgtgaac acggcaaaga atcccgccaa cacgaccgcc agcgcctgtc
     6661 gttgggccgc ccccgggacg cgcttgtcca tcagctcgac ggcgggctcg ccccgcacct
     6721 ccgcccggac catcgcgccc agcaacgcga ccgtcgtgac cttgacgcct ccggcagtgc
     6781 ccgcgctacc acctccgatg aacatcagga ccatcgtcac cagcagggtc ggctcggtca
     6841 gcgcgcctat gtcgaccgcg tcgaatccgg cggtgcgggg gctgaccccc tggaagaacc
     6901 ccgcgaggcc cttcccgggg accgacatct cgccgagcgt cgcggagttg ccccactcga
     6961 acgcgaggac ggctgcggtt ccggccacta tgagcgcgcc ggtcatcacc agcgtgagct
     7021 tcgtgtgcag cgaccagtgc gacgggcgcc gccacgacct cttgacctcg aagagcacag
     7081 ggaagccgat cccgccgacg atgagcccga cggcgatcac gcagatgatc cacgcgtccc
     7141 ccacgaactc cttgaggttg cccgcgaaca ccgagaagcc cgcgttgttg aaggccgaga
     7201 cggcatggaa gacgccgagg tagagagcgc ggcctgcagg ctcgccgtag gcggtcccga
     7261 accgcagagc gaggaccacc gcgctcacgg cctcgaacaa gaagctgacg accgcgacgc
     7321 cgacgacgat ccggcgcacg tccccgaggt cgagcgtctt cgtctcggcc tgcgcgagga
     7381 ggcgcgccct gacgcccagg ttgcggtaga cgacgagagc gagcatcgac gcgagggtca
     7441 tgaacccgaa cccgccgagc tggatcagca cgaggatcgt cccctccccg aagcccgacc
     7501 agtaggcccc ggtgtccacc acggcgaggc ccgtgacgca gaccgccgac gtcgccgtga
     7561 acatcgcggt cgtgaaagac gccgcggcgg gaccctcggt cgcgaccggc aacgagagca
     7621 ggatcgtgcc cacgacgatc gccgcgaaga cggccgcgac gacgaactgc gacgggtgac
     7681 ggagacggaa gcgcgcgagc agccggcgcg aggccccccc gcccgcgacg gttcgaccgg
     7741 acgcggcatg ggagggcatc gcccgacccg atcgaaggac cctccgcccg ccttggtgcg
     7801 tgagcgtgca gccggcgcag tcccgaagca atcccttgcc gggttggaga gactcgcttg
     7861 cccgccgcag actcgggctt acggcttcag tggggaagat gatcgtgaca aaagccctcg
     7921 ccggttcgcg ctcctgggcg ctgctcctgc cgggcgtccc cgtgtgacgt cgccgagggc
     7981 ttcctaagcc cgtaactgac gaggtggctg agctatccaa cagtctcttc gggaggagaa
     8041 aatggcccca caacgaaaca cgcccccggg tgggggcgtg tcgttggacc gacgtatcta
     8101 gcgcgtcgtc agacgcagag gatgagccag aactggccgt cgatcgggcc gtgcttgggg
     8161 tcggacggac acgtgcaggt ccacgcacct gcgttctgga agccgttcct gaactcgacg
     8221 ccgacgcacg tggccgcgcc ggccggcgct gccgttcccc acactgacgc cgccatcaca
     8281 cccgacacga gcaacgcctt gaccgatcgc ttcatggtcc ctcccgccta agggctcctc
     8341 cgcagagccg ccgcgccgga cttcctacac ctccgggtta tcagacgcag a
//
LOCUS       DASQDO010000162        11401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_873262, whole genome shotgun sequence.
ACCESSION   DASQDO010000162 DASQDO010000000
VERSION     DASQDO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873262"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VEV43_04745"
     CDS             <1..940
                     /locus_tag="VEV43_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22859.1"
                     /translation="DGTQGAEGFRIDGDFRPFAGTVGDTDGDHLSDVFVAGYGRVFVV
                     RGKADTLPVDVATFGLGVTRGYEVQHPRRRRDGWSSAAFAGDANGDGLGDLAISAGTT
                     TAQALWVVFGKPDDTKVRVRELGDAGYTIDGAVSLGGLGTGGDVNGDDLDDLVVGDSY
                     ANRAYVVFGQADTDTVPLDDLGTRGFRILGPPTRTRQLAHAAAGRFVAGDVDVDADGL
                     SDFVVSAPEADHRRRRDSGSAYLLFGRRGTNTIRLGRLGDKGIRLDGEDAGDRLGTGL
                     ALAGDVTGDGLPDLLLGEYGERGAGAVHVVSYRLLF"
     gene            complement(944..3232)
                     /locus_tag="VEV43_04750"
     CDS             complement(944..3232)
                     /locus_tag="VEV43_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYP22860.1"
                     /translation="MSKLPAALKRVVLGRPMSSGELEHTLLPKTIALPVFSSDPLSSN
                     AYATQEALIVLVAAGAAGMQFIFPVAIAVATLLAIVVASYRQTVKAYPTGGGAYRVSH
                     ENIGKTAGLLAGSALLFDYTLTVAVSVSAGVEAITSVSATLAEYRVPLAFGFIAFVTV
                     MNLRGVRESGTLFAIPTYGFVLSIFMMIGTGVAQCLGGCPQAESAELVDELHITAGVG
                     AVLLMRAFAAGTTALTGVEAIADGVPAFRYPQSKNAATTLAIMGGLSISMFLGISWLA
                     DHLNVVYVHDPTSAKHQPSVLAQIADTVFSGGPLFYFVQAMSASILILAANTAYQDFP
                     RLASILAADRYLPRQFMNRGDRLVFSNGVILLAAVASVLVYVFEGSLTRLIQLYLIGC
                     FTSFTLSQTGMVIRGRKLKEPGWKKRAVMSGFGAVITGGVLVVISASKFLSGAWIVFT
                     AVPILMFLMRSVNKHYVSVEGELAHPARRPVDRRPGDQHMVIYVTTMDAAVERALGYA
                     RAMRPAHVSAVTFDSSHFRLWKRLAPDIPIELAPQGARRNKAMRAFLTQKRAELGLDQ
                     DDFLTLLIPEILESTSLFEIFRRPALHRLKGSLLTQLGVQVLDVPVVRQDIDPHLDQS
                     HEPGRNYTIVLVSGVHNATLQAFEYAETLQSIDVRAVSFGLDEHQTEKLVEQWASIGI
                     PHPLEIEASPFRDIGTSLVHYLRQFKADGLERTVTVVLPEFVVKKARHQILHNQTALI
                     VKRRLLFERGTVVVSVPYHLER"
     gene            complement(3237..3614)
                     /locus_tag="VEV43_04755"
     CDS             complement(3237..3614)
                     /locus_tag="VEV43_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HYP22861.1"
                     /translation="MAFFKNAVERLARPPEEIRAENLRRWAATVVGVTSIAHLEPRRR
                     CRAAGVIQNIRIDPRPGSGSIEATIIDGTGQMVARWLGRHTISGIRLGMGLIIEGIPG
                     EQTGGELMVLNPEYELVPGPEHG"
     gene            3700..4362
                     /locus_tag="VEV43_04760"
     CDS             3700..4362
                     /locus_tag="VEV43_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYP22862.1"
                     /translation="MQVIIAGCGRVGSELASNLERLGHSVVVVDKSAKAFERLKPGFA
                     GRKVVGFAFDREILEDAGIKEAAAFASVTNGDNSNILSARVAKEHYRVPQVVARIYDP
                     RRAQIYQRLGIQTVATVRWTTDQILRTLLPEEVSVEYTIDNGEVVVTALPAPPEVVGQ
                     KVLSVDVPGRRRVVAVSRFGVPRVPDKDLTVQDGDILHISIVRADAAELGEEIKTVGR
                     PE"
     gene            4359..5039
                     /locus_tag="VEV43_04765"
     CDS             4359..5039
                     /locus_tag="VEV43_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYP22863.1"
                     /translation="MSGERRIRIAIAGAGNVGSYVASDLKRKGHDIVMIEQNKEVIQT
                     TREDLEVSWVNGDACELHTLDAAVLSSCEVVVAATGDDEDNLVISLLAKQEFAVPRVV
                     ARVNHPDNEWLFTESWGVDVAVSTPHLLASLVEEAVSVGDLVRLLNLEQGKVALLEVT
                     LASASPAVGKTVADLNMPPDCTIVAVVRERHVIAPRSETPLQVGDEVLALASPAAEGD
                     LKKALTGE"
     gene            5036..5596
                     /locus_tag="VEV43_04770"
     CDS             5036..5596
                     /locus_tag="VEV43_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYP22864.1"
                     /translation="MIERDAAVALVRKYVHTDVTFRHLVSVEGVMRALARHFGEDAHR
                     WGLAGLFHDLDMDETHGDEERHTQLTIEWLRAEGFDDEQILNAILGHKYETNQTDTMS
                     KAIVHADGVAGLLVACALVRPEKAEGMKVSSVKKKLKEKSFAPGVERDKIRGVEEKIG
                     IPMDEFIAISIGGLQEVAPEIGLTAP"
     gene            complement(5612..6265)
                     /locus_tag="VEV43_04775"
     CDS             complement(5612..6265)
                     /locus_tag="VEV43_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HYP22865.1"
                     /translation="MDAILEPVIHFMEDFVTDYGYLAIFLLMSAESALIPFPSEVTML
                     VGGWYSADGTLSFFWVVTAGVLGNLVGSWLAYALGRKTGRTLLDKYGKYVLIRSHDVD
                     KAEIWWAKHGDAATFFSRLLPVIRTFISLPAGIAKMPFGKFTIYTFLGVVPWTAGLTW
                     LGVVVEDNWEKVLGYFDLPTLIIGALLVAAALWWYLRRRKARAAVAGAAGDGVPPEG"
     gene            6458..8563
                     /gene="topA"
                     /locus_tag="VEV43_04780"
     CDS             6458..8563
                     /gene="topA"
                     /locus_tag="VEV43_04780"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HYP22866.1"
                     /translation="MAKNLVIVESPAKAKTIEKYMGKDYKVLASMGHVRDLPKSDFGI
                     EVNGGVKIAYEPISRATAKKAMTAIRKAAKEAETVWLAPDPDREGEAIAWHVAELAKL
                     KPETTRRVTFNEITKKAVTEAFENPRSIDMALVDAQQARRVVDRIVGYKLSPLLWRNV
                     GPNLSAGRVQSAALFLVVEREREIRAFNPVEYWDLTAKLATEGGDELTALYPVGEKEK
                     FALGDEATATSLKDRVEPGPWTVTAIRKTERRRKPPAPFKTSTLQQAGSSKLGFPTWK
                     TMKLAQGLYEAGHITYMRTDSTNLSNDALGEIGRVVETQFGKEFHEARKFTAKSKGAQ
                     EAHEAIRPASAEVSPTDLAGAVDADALKLYAIIWQRTMASQMAEAVYDATSVDVESNG
                     TTFRATGQILKFKGYMSVYLETGDDETEEAEGLLPELEEGQVLDLRALDAAQHFTQPP
                     PRFTEATLVRRMEEVGIGRPSTYAPTVRLLVDREYVRTESRRLFPTPRGEVVVELLEA
                     HFPDVVDVEFTARMEEDLDQVAVGSREMDPLVREFFESFTAHVAEQSDKMSRPERPTD
                     KTCPNCGRPVVQKFGKHGLWFLSCSGWPECKWSQQLDEHGEPLPDPEGTGEKCPNCGN
                     ELVAKSGRFGPFVGCSNYPECKFIKKEPPKETGEKCPDCGSGLVEKRGRFGPFTGCSN
                     YPECKYIKKKPRKKEEAAS"
     gene            8707..10686
                     /gene="tmk"
                     /locus_tag="VEV43_04785"
     CDS             8707..10686
                     /gene="tmk"
                     /locus_tag="VEV43_04785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYP22867.1"
                     /translation="MLRNGNFFRFFLAQFVSSLGDWLGVVALVVLAQDIGGSIGVGAV
                     MTARVLPGFFVGPIAGVFADRFDRKKTMVISDLMRAGIIFSLPFVENLLYLLLASAVL
                     ESLTLVWGPAKDASLPHFVKPQELTHANSLSLIAVYAPFPLGSVVFAFMATLAAFLAG
                     HFTFFAGLESRPEALALGVNSLSFLFSALMISSLTIPSTRTRIEHMDLGQVKRDLFEG
                     LKFVFEHKQVRPWLLGIALTFTAAGAVFSLGVAFVDDVLGGGDRGFAFLIGFLATGMI
                     IGLLAVGLIARRIQKDVLFSSSLLLVGVGLIGLASVGSLNAAIPLASALGFFGGAAYS
                     TGYSLMHETTTDELRGRTFSAAYTVIRIGTLVGLGLFPFIAGFLGHYQLTSPLGTLDL
                     PGSRITLWLAGGVAVVGGVVSMRAIRAREEAAPGEAAPSAPPRVERGYFVVFEGGDGS
                     GKSTQMAALVRWLEARGADVVTTREPGGTEIGRRVREVLLDPSLASMDARTEALLYAA
                     DRAQHVAEVIRPALEAGKIVVSDRFVDSSLAYQGVARELGLEEVYRISKWATKDLMPD
                     VVFFLSMESRLGLRRIDRDPDRIEQEASTFHDKVGDAYLELARRYPDRFVVLDANRPQ
                     SEVHQDVVEAFEARTSGEDEARLAGFLRDGGFPQR"
     gene            10683..>11401
                     /gene="holB"
                     /locus_tag="VEV43_04790"
     CDS             10683..>11401
                     /gene="holB"
                     /locus_tag="VEV43_04790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HYP22868.1"
                     /translation="MSAWDGVAGVAAAERLAEQLAAGQIAHAWLLTGPRGTGKRAVAF
                     ALAAALVCERGPGVACGECSGCARALRNRHPDVHHVEPEGVIIPVDVVREAVIPEAAR
                     SPFEASYKVFVIEDADRMNDAAQNALLKTLEEPQPDTVFVLVSDHEEEVLETITSRCR
                     VVRLDPVPLERVSEVLVAAGADPDEARRAATLAEGDVARARLLAFDDAVRARVRTWRE
                     LPARLASPVDALDAAAEVVAEA"
BASE COUNT         1826 a         3887 c         3981 g         1707 t
ORIGIN      
        1 cgacggcacg cagggagccg aaggcttccg catcgacggg gacttcaggc cgttcgccgg
       61 aacggtcggc gacaccgacg gcgaccacct gtccgacgtg ttcgtggcgg gctacgggcg
      121 ggtcttcgtc gtccgcggca aggccgacac ccttccggtc gacgtcgcga cgttcggcct
      181 cggcgtcacg cgtggatacg aggtccagca cccacgccgg cgccgcgacg ggtggtcgag
      241 cgctgcgttc gccggcgatg cgaacggaga cgggctgggc gacctcgcga tctcggccgg
      301 gaccacgacc gcacaggccc tgtgggtcgt cttcgggaag ccggacgaca cgaaggtccg
      361 tgtgcgcgag ctgggcgacg ccggctacac catcgacggg gccgtcagcc tcggcgggct
      421 cgggaccggc ggcgacgtca acggagacga cctggacgac ctcgtcgtcg gggactcgta
      481 cgcgaaccgc gcgtacgtcg tgttcgggca ggcggacacg gacacggtcc cgctggacga
      541 cctcgggacg cgcgggttcc ggatcctggg gccaccgacg cgcacgcggc agctggcgca
      601 cgcggctgcc gggcgcttcg tcgcgggcga cgtcgacgtg gacgccgacg ggctgtccga
      661 cttcgtcgtg agcgccccgg aggccgacca ccgccggcgg cgcgactccg gttccgccta
      721 tctcctgttc gggaggcgcg ggacgaacac gatcaggctg ggcaggctcg gcgacaaggg
      781 gatccgcctg gacggcgagg acgccgggga ccgcctcggc acggggctcg cgcttgcggg
      841 cgacgtgacc ggcgacgggc tgccggatct cctgcttggc gaatacgggg agaggggcgc
      901 gggggccgtg cacgtggtct cgtaccgcct gttgttctag cggctaccgc tccaagtggt
      961 acggcacgct caccactacc gtgccccgct cgaacagcag cctccgcttc acgatcagcg
     1021 ccgtctggtt gtgcaggatc tgatgcctcg ccttcttcac cacgaactcc ggcagcacga
     1081 cggtgaccgt ccgctccagc ccgtcggcct tgaactgccg caggtagtgc acgagcgacg
     1141 tcccgatgtc gcggaacgga gacgcctcga tctccagggg atgcgggatc ccgatcgacg
     1201 cccactgctc gacgagcttc tccgtctggt gctcgtcgag tccgaacgac accgcgcgta
     1261 cgtcgatcga ctgcagcgtc tccgcgtact cgaacgcctg cagcgtcgcg ttgtgcacgc
     1321 cggacaccag cacgatcgtg tagttgcggc ccggctcgtg gctctggtcg aggtgcgggt
     1381 cgatgtcctg gcgcacgacg ggcacgtcga gcacctggac ccccagctgc gtgagcaacg
     1441 accccttcag gcggtggagc gcgggccggc ggaagatctc gaacaggctc gtgctctcga
     1501 ggatttcggg gatcagcagc gtgaggaagt cgtcctggtc gaggccgagc tcggctctct
     1561 tctgcgtcag gaacgcgcgc atcgccttgt tccgtcgcgc cccctggggc gcgagctcga
     1621 tcgggatgtc cggcgcgagc cgcttccaca gacggaagtg tgagctgtcg aacgtgacgg
     1681 ccgagacgtg cgcgggccgc atcgctcgcg cgtaccccag cgctcgctcg accgccgcgt
     1741 ccatcgtcgt gacgtagatc accatgtgct ggtcgcctgg acggcggtcg accggccggc
     1801 gcgcggggtg cgcgagctcc ccctcgaccg agacgtagtg cttgttcacc gagcgcatca
     1861 ggaacatcag gatcgggacg gccgtgaaca cgatccaggc gcccgagagg aacttcgacg
     1921 cgctgatcac cacgaggacg cctccggtga tcaccgctcc gaagccgctc atcaccgccc
     1981 gcttcttcca ccccggttcc ttcagcttcc tcccgcggat gaccatcccg gtctgcgaga
     2041 gcgtgaacga cgtgaagcac ccgatgaggt agagctggat caggcgcgtc aagctcccct
     2101 cgaacacgta gacgaggaca cttgcgacgg cggcgagaag gatcacgccg ttcgagaaca
     2161 cgaggcggtc gccgcggttc atgaactgac gggggagata gcggtccgcc gccaggatcg
     2221 aagcgagcct cgggaagtcc tggtaagcgg tgttggcggc gaggatcagg atcgacgccg
     2281 acatcgcctg cacgaagtag aagagcggac cgccgctgaa gaccgtgtcc gcgatctggg
     2341 caaggacgga cggctggtgc ttcgccgacg tggggtcgtg cacgtagacg acgttcaggt
     2401 ggtcggcgag ccacgagatc ccgaggaaca tcgagatcga gagcccgccc atgatcgcca
     2461 gcgtcgtcgc ggcattcttc gactgcgggt accggaaagc cggaacgccg tccgcgatgg
     2521 cctcgacgcc cgtcagagcg gtggtcccgg cggcgaacgc cctcatcagc aacaccgctc
     2581 cgacgccggc ggttatgtgc agctcgtcga ccaattctgc gctctctgcc tgagggcatc
     2641 ccccgaggca ttgcgcgacg cccgtgccga tcatcatgaa gatcgacagg acgaacccgt
     2701 acgtcggtat ggcgaagagc gtcccggact cccggacgcc gcgcaggttc atcaccgtca
     2761 cgaaggcgat gaatccgaac gccagcggca cgcggtactc cgccagtgtc gcggacacgg
     2821 acgtgatcgc ctcgactccg gccgagaccg acaccgcgac cgtcagtgtg tagtcgaaca
     2881 gcagagcgga accggccagc aggcctgcgg tcttcccgat gttctcgtgc gacacgcggt
     2941 aggcgccccc gccggtcgga taggccttga cggtctggcg gtagctcgcg accacgatcg
     3001 cgagcagcgt ggcgacggcg atcgcgaccg ggaagatgaa ctgcatcccg gccgcccctg
     3061 cggccacgag gacgatcagc gcctcctgcg tcgcgtacgc gttggacgac agcgggtcgc
     3121 tggagaacac cgggagcgcg atggtcttcg ggagcagcgt gtgctcgagc tcgccggagg
     3181 acatcgggcg gccgaggacc acgcgcttga gcgcggcggg gagcttgctc atcgcgtcag
     3241 ccgtgctccg gtcccggcac gagctcgtac tccgggttga gcaccatcag ctcgcctccg
     3301 gtctgctcgc ccgggatccc ttcgatgatc aagcccatcc cgagcctgat acccgagatc
     3361 gtgtgacgcc cgagccagcg cgccaccatc tgcccggtcc cgtcgatgat cgtcgcttcg
     3421 atcgatccgc ttcccggacg cgggtcgatc ctgatgttct ggatgacgcc cgcggcgcgg
     3481 cagcggcggc gtggctcgag gtgcgcaatg gaggtgactc cgaccaccgt cgcggcccat
     3541 cgccggaggt tctccgccct gatctcctcg ggcggccggg cgagtcgctc gaccgcgttc
     3601 ttgaagaagg ccacggagcg caagtatggc agtatcgccc gccggggggc tgacgccggc
     3661 ccgcgtattt tcctaacagg agaggagccc gcgctcgagg tgcaggtgat catcgccggc
     3721 tgcggccgcg tcggatcgga gcttgcgtcg aacctcgaac ggctcgggca ctccgtcgtc
     3781 gtcgtcgaca agtcggccaa ggctttcgag cgactcaagc ccggtttcgc gggaaggaag
     3841 gtcgtcgggt tcgcgttcga ccgggagatc ctcgaagacg ccggtatcaa ggaggccgcc
     3901 gcgttcgcgt cggtgaccaa cggcgacaac tcgaacatcc tgtccgcacg ggtcgcgaag
     3961 gagcactacc gcgtcccgca ggtcgtcgcc cgtatctacg acccgcgccg cgcgcagatc
     4021 taccagcggc tcggcatcca gaccgtggcg accgtgcgat ggacgaccga ccagatcctc
     4081 cggacgctcc tgcccgagga ggtctcggtc gagtacacga tcgacaacgg cgaggtcgtc
     4141 gtgacggcgc tgcccgcacc gcccgaggtc gtcgggcaga aggtgctgtc ggtggacgtg
     4201 cccggccgcc gccgcgtggt cgcggtcagc cgcttcggcg tcccccgcgt gcccgacaag
     4261 gacctgacgg tccaggacgg cgacatcctc cacatctcga tcgtccgcgc cgacgcggcc
     4321 gagctcggcg aggagatcaa gaccgtgggg cgccccgagt gagcggcgag cgcaggatcc
     4381 gtatcgcgat cgcgggggcc ggcaacgtcg gcagctacgt ggccagcgac ctcaagcgca
     4441 agggccacga catcgtgatg atcgagcaga acaaggaggt catccagacc acccgcgagg
     4501 acctcgaggt cagctgggtc aacggcgacg cgtgcgagct gcacacgctc gacgcggccg
     4561 tgctctcttc ttgcgaggtc gtcgtcgcgg cgacgggaga cgacgaggac aacctcgtga
     4621 tctcgctgct ggccaagcag gagttcgcgg tgccgcgggt cgtcgcccgc gtgaaccacc
     4681 ccgacaacga gtggctgttc accgagtcgt ggggcgtcga cgtcgcggtc agcacgccgc
     4741 acctgctcgc gtctctcgtc gaggaggccg tctccgtcgg cgacctcgta cgtctgctca
     4801 atctcgaaca gggcaaggtc gcgctgctcg aggtcacgct cgcgtcggcg tcccccgcgg
     4861 tgggcaagac cgtcgccgac ctgaacatgc ccccggactg cacgatcgtc gcagtcgtgc
     4921 gcgagcgtca cgtaatcgcg ccgaggtccg agacgccgct gcaggtgggt gacgaggtgc
     4981 tcgcgctcgc gtcgccggcc gccgagggcg acctcaagaa ggcgctgacg ggcgaatgat
     5041 tgagcgcgac gccgcggtcg cgctcgtgcg caagtacgtg cacaccgacg tcaccttccg
     5101 ccacctcgtc tcggtcgaag gcgtgatgcg cgcgctggcc cggcacttcg gcgaggacgc
     5161 gcaccgctgg ggtctggccg ggctcttcca cgacctcgac atggacgaga cgcacggcga
     5221 cgaggaacgg cacacgcagc tcacgatcga gtggctgagg gccgagggct tcgacgacga
     5281 gcagatcctg aacgcgatcc tggggcacaa gtacgagacc aaccagacgg acacgatgtc
     5341 gaaggcgatc gtgcacgcgg acggagtggc ggggctgctg gtcgcgtgcg cgttggtgag
     5401 gccagagaaa gccgagggga tgaaggtctc gtcggtgaag aagaagctga aggagaagtc
     5461 gttcgcgccc ggcgtcgagc gggacaagat ccgcggcgtc gaggagaaga tcgggatccc
     5521 gatggacgag ttcatagcca tctcgatcgg gggcctgcaa gaggtagcgc cggagatagg
     5581 cctcaccgcg ccctgaccac gtgggtgtcc gctagccctc gggcgggacc ccgtcgccag
     5641 cggcccccgc taccgcggcc ctcgccttgc gacgccgtag gtaccaccac agggcggccg
     5701 cgaccagcag cgcgccgatg atcagcgtcg gcaggtcgaa gtagcccagg accttctccc
     5761 agttgtcctc gaccacgacg ccgagccacg tcaggcccgc cgtccacggc acgacgccca
     5821 ggaacgtgta gatcgtgaac ttgccgaacg gcatcttcgc gatccccgcc gggagcgaga
     5881 tgaacgtccg gatcaccggc aggagccgcg agaagaacgt cgcggcgtcg ccgtgcttgg
     5941 cccaccagat ctcggctttg tcgacgtcgt ggctccggat cagcacgtac ttcccgtact
     6001 tgtcgaggag cgtccggccc gtcttgcgcc cgagggcgta tgcgagccac gaacccacga
     6061 ggttcccgag caccccggcc gtcacgaccc agaagaagga gagggtcccg tcggcggagt
     6121 accagccccc gacgagcatc gtcacctcgc tcgggaacgg gatcagtgcc gactcggcgc
     6181 tcatcaggag gaagatcgcc aggtacccgt agtccgtgac gaagtcctcc atgaagtgga
     6241 tgacgggttc caggatggcg tccacgggcg ccgatggtag tcggatttcg cgagcgcgaa
     6301 agccctggtc agggggccag ggaagccccg gggggccgga ggcgttggtt gacaacgact
     6361 gccgagcaat ggcataagcg gcccccggac cgagtccgga acgagagcgg gccgcctcac
     6421 cggcggaatt ttccgccgca gcagaccaag gagccgtgtg gccaagaacc tcgtaatagt
     6481 cgagtcgcct gcgaaggcga agacgatcga gaagtacatg ggcaaggact acaaggtgct
     6541 tgcctcgatg gggcacgtcc gcgacctgcc gaagtccgac ttcgggatcg aggtcaacgg
     6601 cggcgtcaag atcgcgtacg agccgatctc ccgcgccacc gcgaagaagg cgatgacggc
     6661 gatccgcaag gccgccaagg aggccgagac cgtctggctc gcccccgacc ccgaccgcga
     6721 gggcgaagcg atcgcctggc acgtagccga gctcgccaag ctgaagcccg agacgacgcg
     6781 tcgcgtcacg ttcaacgaga tcacgaagaa ggccgtgacc gaggccttcg agaaccctcg
     6841 ttcgatcgac atggcgctcg tggacgcgca gcaggcccgt cgcgtcgtcg accgcatcgt
     6901 cggctacaag ctctcgccgc tgctgtggcg caacgtcggc ccgaacctgt cggcgggccg
     6961 cgtgcagagc gcggccctct tcctcgtggt cgagcgtgag cgcgagatca gggcgttcaa
     7021 ccccgtcgag tactgggacc tgaccgcgaa gctggcgacg gagggcggcg acgagctgac
     7081 cgcgctctat cccgtcggcg agaaggagaa gttcgctctg ggcgacgagg cgacggcgac
     7141 gtcgctcaag gatcgcgtcg agcccggccc ctggaccgtc accgcgatcc gcaagaccga
     7201 gcgtcgccgc aagccgccgg cgccgttcaa gacgtcgacg ctccaacagg cagggtccag
     7261 caagctcggc ttcccgacgt ggaagacgat gaagctggcg cagggcctgt acgaggccgg
     7321 ccacatcacc tacatgcgaa cggactcgac gaacctctcg aacgacgcgc tcggcgagat
     7381 cggccgcgtc gtcgagaccc agttcggcaa ggagttccac gaggcccgca agttcaccgc
     7441 taagtccaag ggggcccagg aggcacacga ggcgatcagg cccgcgtcgg ccgaggtctc
     7501 cccgaccgac ctggcggggg ccgtcgacgc ggacgcgctg aagctgtacg cgatcatctg
     7561 gcagcgcacg atggcctcgc agatggccga agccgtttac gacgcgacgt ccgtggacgt
     7621 cgagtccaac gggacgacgt tccgcgccac cgggcagatc ctcaagttca aggggtacat
     7681 gagcgtctac ctcgagaccg gcgacgacga gaccgaggag gccgagggcc ttctgccgga
     7741 gctcgaggag ggccaggtgc tcgatctccg cgccctcgac gcggcgcagc acttcaccca
     7801 gcccccgccg cgcttcaccg aggcgacgct cgtccgccgc atggaggagg tcggcatcgg
     7861 ccgcccgtcg acgtacgcgc cgacggtccg cttgctcgtc gaccgcgagt acgtccgcac
     7921 cgagtcgcgg cgactgttcc cgacgccgcg cggcgaggtc gtggtcgagc tcctcgaggc
     7981 ccacttcccg gacgtcgtcg acgtcgagtt caccgcgcgc atggaggagg acctcgacca
     8041 ggtcgcggtc ggctcgcgcg agatggaccc gctcgtgcgc gagttcttcg agtcgttcac
     8101 cgcgcacgtc gccgagcaga gcgacaagat gtcgcggccc gagcgcccga ccgacaagac
     8161 ctgtcccaac tgcggccgtc cggtcgtcca gaagttcggc aagcacgggc tgtggttcct
     8221 gtcgtgctcg ggctggcccg agtgcaagtg gtcgcagcag ctcgacgagc acggcgagcc
     8281 gctcccggac cccgagggca cgggcgagaa gtgcccgaac tgcggcaacg agctcgtggc
     8341 gaagtccggc cgcttcggcc ccttcgtcgg gtgctccaac taccccgagt gcaagttcat
     8401 caagaaggag ccgccgaagg agacgggcga gaagtgcccg gactgcggat ccggcctcgt
     8461 cgagaagcgg ggacggttcg gccccttcac cggctgctcg aactaccccg agtgcaagta
     8521 catcaagaag aagccgcgca agaaggaaga agccgcctcc tgaggctacg ctgccccccg
     8581 gctacccggc cctagactca gggcgtcgac tgggcggcgc ggagcatctg gaggctgacg
     8641 atccaccaac ccgaggcaga cctgcacgtc gcagagcccg ccgaagaagg cgtctcgtac
     8701 ctctcgctgc tccgcaacgg gaacttcttc cggttcttcc tcgcgcagtt cgtgtcgagc
     8761 ctcggcgact ggctcggcgt cgtcgccctg gtcgtgctgg cccaggacat cgggggctcg
     8821 atcggcgtgg gggcggtcat gaccgcccgc gtcctccccg ggttcttcgt cggcccgata
     8881 gcgggtgtgt tcgcggaccg tttcgaccgc aagaagacga tggtgatctc ggacctgatg
     8941 cgcgccggga tcatcttctc gcttccgttc gtcgagaacc tgctctacct gctgctcgcc
     9001 tccgcggtcc tcgagtcgct gacgctggtg tgggggccgg cgaaggacgc gtcgctgcct
     9061 cacttcgtca agcctcagga gctgacgcat gcgaactcgc tgtcgttgat cgcggtctac
     9121 gcgccgttcc cgctcggctc cgtcgtcttc gccttcatgg cgacgctggc cgcgttcctg
     9181 gccgggcatt tcacgttctt cgccgggctc gagagcaggc ccgaagcgct cgccctaggc
     9241 gtcaactcgc tctcgttcct gttctcggcc ctcatgatct cgagcctgac gatcccgtcg
     9301 acgcgaacgc ggatcgagca catggacctg ggccaggtga agcgcgacct gttcgagggc
     9361 ctgaagttcg tgttcgagca caagcaggtg cggccgtggt tgctcgggat cgcgctgacg
     9421 ttcaccgctg cgggggccgt gttctcgctc ggcgtcgcgt tcgtcgacga cgtcctcggc
     9481 ggcggcgacc gtggcttcgc gttcctgatc gggttcctcg cgaccggcat gatcatcggt
     9541 ctgctcgcgg tgggcctgat cgcgcgccgg atccagaagg acgtcctgtt ctcgtcgtcg
     9601 ctgttgctcg tgggggtagg gctgatcgga cttgccagcg tcggcagcct caacgcggcg
     9661 atcccgctgg cgagcgcgct cggcttcttc ggcggcgcgg cgtactccac ggggtactcg
     9721 ctcatgcacg agacgacgac ggacgagctg cggggccgga cgttcagcgc cgcctatacc
     9781 gtgatccgca tagggacgct ggtcgggctc gggctcttcc ccttcatcgc cgggttcctg
     9841 ggtcactacc agctgacgtc gcctctgggg acgctcgacc tcccgggcag ccgcataacg
     9901 ctgtggctcg ccggcggcgt cgccgtcgtc ggtggcgtcg tgtccatgcg cgccatccgg
     9961 gcgcgcgagg aagccgcccc gggggaagcc gccccttcgg cgccacctcg ggtcgagcgc
    10021 ggctacttcg tcgtcttcga gggcggcgac ggctccggca agagcacgca gatggcggcg
    10081 ctcgtccggt ggctcgaggc acgcggcgcc gacgtcgtga ccacgaggga gccgggcggc
    10141 accgagatcg gccgcagggt acgcgaggtc ctcctcgacc cctcgctcgc gtcgatggac
    10201 gcgcgcaccg aggccctgct gtacgcggcc gaccgagccc agcatgtggc ggaggtcatc
    10261 cgtcctgcgc tcgaggcggg gaagatcgtc gtgtcggatc gtttcgtgga ctcttcgctc
    10321 gcgtaccagg gcgtggcgcg cgagctcggg ctcgaggagg tgtaccggat cagcaagtgg
    10381 gcgacgaagg acctcatgcc ggacgtcgtc ttcttcttga gcatggagtc gcgcctcggc
    10441 ctgcggcgca tcgaccgcga ccccgaccgc atcgagcagg aggcgtcgac gttccacgac
    10501 aaggtcggtg acgcgtacct cgagctcgcc cgccgctatc ccgaccggtt cgtcgtgctc
    10561 gacgcgaacc gcccgcagtc cgaggtccac caggacgtcg tcgaggcgtt cgaggcgcgg
    10621 acctcgggcg aggacgaggc gcggctcgcg ggcttcctgc gcgacggcgg gttcccccag
    10681 cggtgagcgc ctgggacggc gtcgccggcg tcgcggcggc cgagcgactc gccgagcagc
    10741 tggccgccgg ccagatcgca cacgcgtggc tcctgacggg gccgcgcggc accggcaagc
    10801 gcgcggtcgc gttcgcgctc gccgcggctc tcgtgtgcga gcgggggccg ggcgtcgcct
    10861 gcggggagtg ctccggatgc gcccgcgctc tgcgcaaccg gcatcccgac gtgcaccacg
    10921 tcgagcccga gggcgtgatc atcccggtcg acgtcgtgcg cgaggccgtc ataccggagg
    10981 ccgcgcgttc gccgttcgag gcctcgtaca aggtcttcgt catcgaggac gccgaccgca
    11041 tgaacgacgc cgcgcagaac gccctgctga agacgctcga ggagccgcag cccgacaccg
    11101 tgttcgtgct cgtctcggac cacgaggagg aggtgctcga gacgatcact tcccgttgcc
    11161 gggtcgtgcg gctcgacccc gtcccgctcg agcgcgtctc cgaggtgctg gtcgccgccg
    11221 gagcggaccc cgacgaggcg cggcgcgcgg cgacgctggc cgaaggcgac gtagcgcggg
    11281 ctcgtctcct cgcgttcgac gacgccgtgc gggcgcgcgt gcggacgtgg cgagagcttc
    11341 ccgcccggct ggcctcgccc gtcgacgcgc tcgacgcggc cgccgaggtc gtcgccgagg
    11401 c
//
LOCUS       DASQDO010000163         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_322522, whole genome shotgun sequence.
ACCESSION   DASQDO010000163 DASQDO010000000
VERSION     DASQDO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322522"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VEV43_04795"
     CDS             complement(<1..1204)
                     /locus_tag="VEV43_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYP22869.1"
                     /translation="MAAPSDRTATTPAPAPPESPPPPERAPAREPTPAPQPPGRGAPR
                     GVKAVLKKVRPAKSPATPALDAIVKEVKARRAKADLKIVERANEIAEAYHSGQMRKSG
                     DPFITHPLGVALSLAKLGLDETTVAAGLLHDAVEDTELTLAEVEAELGFEVAQLIDGV
                     TKLDKMRLRSAEHGRAENLRKMIVATAKDVRVLLVKIADRLHNMQTLAPLAPEKRKVI
                     ANETLEIYAPLAHRLGMYAVKWQLEDLAFMTMHPKRYAEIEALVQQRQPERERVLDEV
                     VTQILDKMKDMKIKAEVFGRPKNLYAIYEKIVLRGKQFEEIFDLVGIRVVVESVKDCY
                     GALGALHTLFTPVPGRFKDYIAMPKFNLYQALHTTVMGPGGRVMEIQIRTEAMHRAAE
                     TGIAAHWLY"
     gene            complement(1238..1534)
                     /locus_tag="VEV43_04800"
     CDS             complement(1238..1534)
                     /locus_tag="VEV43_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22870.1"
                     /translation="MSRTEDAPKRRSLSYIRLAAFLASQPANRDAIELGVDEIADLVD
                     EELPANARFPSWWRNDPKRMHSRAWLTAGWEVKHMGGGRPSVLFVRRKDAEAAP"
     gene            complement(1600..2130)
                     /locus_tag="VEV43_04805"
     CDS             complement(1600..2130)
                     /locus_tag="VEV43_04805"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HYP22871.1"
                     /translation="MSPGTHDWLKEHIRDIPDFPKPGVVFKDITPLLADKKAFAYTID
                     AISHEFDRDEIDKVVGIEARGFMVAAPLAYRFTAGLIPVRKKGKLPWEVASQEYSLEY
                     GVDHLEMHRDAIEPGERVLVVDDVLATGGTAAATAALIERCGGKVSALATIMELTFLG
                     GRARLEGYEFLSLISY"
     gene            complement(2127..3275)
                     /gene="secF"
                     /locus_tag="VEV43_04810"
     CDS             complement(2127..3275)
                     /gene="secF"
                     /locus_tag="VEV43_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYP22872.1"
                     /translation="MMDALRRLYRGETKIDFIGKRNIWFAISGAILVICLGSLILRGF
                     DYGIEFEGGVAIQAPIAADGPVADLSSAEVIGEVRGALAEFDADDAQIQVEEATDGGD
                     RDVIVQTAEIADPERQQEVVNAVSEAVGATIEETDSQRIGSKWGAEITDKAVRALVIF
                     VFVILAFISWRFDWKMGVAAIIALLHDLLITAGVYSLVGFEVTPSTVIAILTILGYSL
                     YDTVVVFDKVEEDADAYAGTGRMTYQGAANLAMNQVFARSLNTSMTTLIPVAALLFVG
                     AGLAGAATLKDLALALFVGILGGTYSSMYIATPVLSVMKEHEPRYRNVREKMQRAAER
                     AEARGTDVVSPVEAAAGVGSAPVRPAAPAAGPARARAGSKKAKRRRQR"
     gene            complement(3272..>3608)
                     /locus_tag="VEV43_04815"
     CDS             complement(3272..>3608)
                     /locus_tag="VEV43_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYP22873.1"
                     /translation="GIAADSFIVAFERLKDEVRSGKSLRAAVQRGSDRAIKTILVADF
                     VTGIAAVILFFLAVGPVRGFALTLGLSTLVDVLLAFFLIRPAMVLLGRTKHLGGAAVG
                     ARDTAEAPA"
BASE COUNT          530 a         1231 c         1241 g          606 t
ORIGIN      
        1 tgtagagcca gtgggccgcg atgcccgtct cggccgcgcg gtgcatcgcc tcggtccgga
       61 tctggatctc catcacgcgg ccccccggcc ccatcacggt ggtgtgcagg gcctggtaca
      121 ggttgaactt gggcatcgcg atgtagtcct tgaaccgccc cggcacgggg gtgaacagcg
      181 tgtgcagcgc gccgagcgcc ccgtagcagt ccttcaccga ctcgacgacg acgcggatgc
      241 cgaccaggtc gaagatctcc tcgaactgct tgccgcggag cacgatcttc tcgtagatcg
      301 cgtaaaggtt cttggggcgg ccgaagacct ccgccttgat cttcatgtcc ttcatcttgt
      361 cgaggatctg cgtgacgacc tcgtcgagca cacgctcgcg ctccggctgc cgctgctgca
      421 cgagcgcctc gatctccgcg tagcgcttcg ggtgcatggt catgaacgcg aggtcctcga
      481 gctgccactt caccgcgtac atcccgagcc ggtgcgcgag gggggcgtag atctcgaggg
      541 tctcgttcgc gatcaccttg cgcttctccg gggccagcgg cgcgagcgtc tgcatgttgt
      601 gcagccggtc ggcgatcttc acgaggagca cgcggacgtc cttggccgtc gcgacgatca
      661 tcttgcggag gttctcggct cgcccgtgct cggcggagcg cagccgcatc ttgtcgagct
      721 tcgtgacccc gtcgatcagc tgcgccacct cgaagccgag ctcggcctcg acctcggcca
      781 gggtgagctc cgtgtcctcg acggcgtcgt gtagcagccc tgccgcgacc gtggtctcgt
      841 cgaggcccag cttcgcgagc gagagcgcga cgccgagagg gtgcgtgatg aacgggtcgc
      901 cggacttgcg catctggccc gagtggtacg cctcggcgat ctcgttggcg cgctcgacga
      961 tcttcaagtc ggccttggcg cggcgggctt tgacctcctt gacgatcgcg tcgagggcag
     1021 gcgtagccgg agacttcgcc ggccgcacct tcttgaggac ggccttgacg ccgcgcggtg
     1081 cgccacggcc gggaggctgc ggggccgggg tgggctcgcg cgccggcgcc cgttcggggg
     1141 gcggcgggct ctcaggcggc gcgggggccg gggtcgtcgc ggtgcggtcg gacggtgctg
     1201 ccatacccca ttgtcgcgtc taagggcggg ctttgggcta aggcgcggct tcggcgtcct
     1261 tacggcgcac gaagaggacg ctcggtctgc cgccgcccat gtgcttgacc tcccagcccg
     1321 cggtcagcca ggcgcggctg tgcatgcgct tggggtcgtt gcgccaccac gacgggaagc
     1381 gcgcgttcgc ggggagctcc tcgtcgacca ggtccgcgat ctcgtcgacg ccgagctcga
     1441 tcgcgtcccg gttagccggc tgggacgcga ggaacgcggc gagacggatg tagctgagcg
     1501 agcggcgttt gggcgcgtct tcggtccggg acatgtcgaa acagagcgta aggattcgag
     1561 tcgacacaag acagggggaa ggctcttatg tccgttatgt cagtacgaaa tgaggctcag
     1621 gaactcgtag ccctccagcc gcgcccggcc cccgagaaac gtcagctcca tgatcgtcgc
     1681 aagggccgag accttgcccc cgcagcgttc gatcagtgcc gccgtcgccg ctgcagtccc
     1741 tccggtcgcg agcacgtcgt ccacgaccag cacgcgctcg cccggctcga tcgcgtcccg
     1801 gtgcatctcg agatggtcga cgccgtactc gagcgagtac tcctgcgacg cgacctccca
     1861 ggggagcttg cccttcttgc ggacggggat cagcccggcg gtgaagcggt atgccagggg
     1921 ggccgccacc atgaacccgc gcgcctcgat cccgacgacc ttgtcgatct cgtcgcggtc
     1981 gaactcgtgc gagatcgcgt cgatcgtgta ggcgaacgcc ttcttgtcgg cgagcagcgg
     2041 cgtgatgtcc ttgaagacga cccccggctt cgggaagtcc gggatgtccc ggatgtgctc
     2101 cttcagccag tcgtgcgtcc ccgggctcat ctctgtctcc ggcgcttcgc tttcttcgaa
     2161 ccggcgcgcg cacgggccgg gcccgccgcc ggcgcggccg gccgcaccgg ggcgctgccc
     2221 actcctgccg cggcctcgac cggggagacc acgtcggtcc cgcgggcctc cgctcgctcg
     2281 gcggcgcgct gcatcttctc gcgcacgttg cggtaccggg gctcgtgctc cttcatcacc
     2341 gagagcacgg gggtcgcgat gtacatggac gagtaggtgc cgcccaggat cccgacgaag
     2401 agcgcgagcg cgaggtcctt gagggtcgcg gcccccgcca ggccggcacc gacgaacagc
     2461 agcgccgcga ccgggatcaa cgtcgtcatc gacgtgttca gcgaccgcgc gaacacctgg
     2521 ttcatcgcga ggttggccgc gccctggtac gtcatccgtc cggtccccgc gtacgcgtct
     2581 gcgtcctcct cgaccttgtc gaagacgacc acggtgtcgt agagcgagta accgaggatg
     2641 gtcaggatcg cgatcacggt ggacggcgtg acctcgaaac cgacgaggga gtagaccccc
     2701 gcggtgatca gaaggtcgtg cagcagcgca atgatcgcgg cgacccccat cttccagtcg
     2761 aagcgccacg agatgaaagc gaggatcacg aacacgaaga tcacgagggc gcggacggcc
     2821 ttgtccgtga tctcggctcc ccatttgctg ccgatgcgct gactgtcggt ctcctcgatc
     2881 gtcgcgccga cggcctcgct gaccgcgttg acgacctcct gctgtcgctc cgggtcggcg
     2941 atctccgccg tctgcacgat gacgtcgcga tccccgccgt cggtggcctc ctcgacctgg
     3001 atctgcgcgt cgtcggcgtc gaactccgcc agcgcgccgc ggacctcccc gatgacctcg
     3061 gcgctggaga ggtctgccac gggcccgtct gcggcgatgg gggcctggat ggcgacgcca
     3121 ccttcgaact cgatcccgta gtcgaagccg cggaggatca gcgaccccag gcagatcacg
     3181 aggatggcgc cggagatcgc gaaccagatg ttgcgcttgc cgatgaagtc gatcttggtc
     3241 tcgcccctgt agaggcgccg gagtgcgtcc atcatgcggg tgcctccgcg gtgtcccggg
     3301 ccccgacggc cgcgccgccc aagtgcttgg tgcggccgag caggaccatc gccggccgga
     3361 tcaggaagaa ggcgagtagg acgtcgacga gggtggacag cccgagcgtc aaggcgaacc
     3421 cgcgcaccgg ccccaccgcg aggaagaaca ggatcaccgc ggctatgccg gtgacgaaat
     3481 cggcgacgag gatcgtcttg atcgcgcggt ccgacccccg ttgcaccgcg gcccggaggc
     3541 tcttgccgga acggacctcg tccttcagcc gctcgaacgc gacgatgaac gagtcggcgg
     3601 cgatccct
//
LOCUS       DASQDO010000164         6894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_751374, whole genome shotgun sequence.
ACCESSION   DASQDO010000164 DASQDO010000000
VERSION     DASQDO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751374"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VEV43_04820"
     CDS             complement(<1..759)
                     /locus_tag="VEV43_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22874.1"
                     /translation="MGLRSRALHLYVAALAAALVVVLLGSTTDPAPGREVAAFAVLTI
                     LFALTELTDLTFHGKGGDWGLSASEALLLPMVVVLPAGELVWAVTLAIAAARVYRRKA
                     GWLKGIFNVAQYGCGAAAAAGVWYALSDPAPALTLRNAAVAVLAVLTFTVLTHVFVAI
                     VMAIAGNARLTSALDGVWTPALINLAGSVVLGLLFASAILSSRWTVALFPLPLLGLYL
                     GYRAVLRQRDEAERLRPLHAASRALAGRPERDEAR"
     gene            complement(761..2491)
                     /locus_tag="VEV43_04825"
     CDS             complement(761..2491)
                     /locus_tag="VEV43_04825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYP22875.1"
                     /translation="MHSKTMPPPTASVRRGTLAGALLLLVATLPVSGPAGSRSMLEVV
                     VQGAGSALFAAEAVTSSGGAVDAVLPIVDGVSARIPAERLDELRADPRVRSVTADDPI
                     AFDGAPDTGTAHRIQKIVRSNDLWARGIDGAGTTVALLDTGVYAAHPDLAGRVVHCED
                     LSHEAGTEAHCADTFGHGTFMAGLIAGDGSASHGKHTGAAPGARIVSVKAAGFDGATD
                     VSTVLAGIQWIVAHKDVYGIRVMNLSLGTDSAQDDRLSPLNYAVERAWASGIVVVVSA
                     GNSGPDAGTVMKPGDDPYVITVGASDDEGSMATGDDRVPVFSSRGPTRANGLAKPDLV
                     SPGVHTVSLRSPGSAIDQRYGSTAAIGGHYFKGTGTSMATATVSGIVAQLLQDEPGLT
                     PDQVKARLTSTARGIAESDRMRAGAGLVDAFAAATSTSMAAANQGLGQATGLGLLEGD
                     RGSAGVEVVTPLGQIFLQGEYVAQTDPDEVSVTNPAGLVPWAGATWKADGWDAESWAG
                     ATWKSGDWAGATWKGATWKATVWDGATWKGATWKNADWDGATWKGATWKGATWKATSW
                     QTRRYAAAWE"
     gene            2666..3652
                     /gene="speB"
                     /locus_tag="VEV43_04830"
     CDS             2666..3652
                     /gene="speB"
                     /locus_tag="VEV43_04830"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HYP22876.1"
                     /translation="MRTGRDWEPAFAGATTFQKVPLALDEESLQGADVAIVGAPMDEY
                     VTHRPGARFGPRAIRTAIDGGGPPKTWHMDLGVDPFAELRVVDHGDAEVVPGDPAASH
                     VAISAAVGRVVAAGAVPVVLGGDHSIAYPDVAAVAASLPKGSLAVVQFDTHTDTATEN
                     WGVARAHGTPFRHLVDEGVLPGERLVQVGLRGYWPNPDEWAWARAAGVGWHRMEDVID
                     RGIDAVIDDVLERIAPAEHLFLSVDVDVLDPAFAPGTGTPEPGGMSTRVLLRAIRKLT
                     VAKGLAGMDVVEVSPPYDHADITAMAAHRIVVDALAGLAVHRRGGDVRPERA"
     gene            3654..4436
                     /locus_tag="VEV43_04835"
     CDS             3654..4436
                     /locus_tag="VEV43_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HYP22877.1"
                     /translation="MPAPGPVPGEDDLLERVAGLVRSAGRVVALTGAGISTESGIPDF
                     RGPQGVWTKDPKAERLSTLKAYMEDPELRVFAWRSRMEHPAWTATPNAGHLALAQLER
                     KGKLHTLVTQNIDGLHQKAGTSPDRLVEIHGTLREVMCMSCDERAPMEAALERVRAGE
                     ADPACRTCGGILKSATISFGQALNEADMRRAHEAAESCDLFLAIGTSLTVYPVAYLPA
                     VAAGAGATVVVMNAEPTRYDAAADVVVRTPVGRALSEIAARV"
     gene            complement(4433..5755)
                     /locus_tag="VEV43_04840"
     CDS             complement(4433..5755)
                     /locus_tag="VEV43_04840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP22878.1"
                     /translation="MKDYRSYSFWLDTCGDDLTPRPSLRSPETVDVAIVGAGYTGLLT
                     ADYHKQADPSLRVAIVEKEIAGFGASGRNGGWCSALFAGARSAKDMHGPLAATVDEVG
                     RVVDAEGIDAHWHKGGTLTLATSPAHVPRVRDEAASSDARWLGEAEVAERIRVPGCLG
                     AAFTPHCARVHPARLVRGLARVVEGMGVSICERTPALGIERGRIDVPGASLTADVVVR
                     ATEGYSVTLPRMRRRVVPLYSLMIVTEPLSESFWEHVGWAGAETITDGRHLLVYAQRT
                     ADDRIAFGGRGAPYHYGSRISDSFDDEPKVFAELERVLHELWPDARGARITHRWGGAL
                     GVPRDWYSSVGFDRAAGLAWAGGYVGDGVSTSNLAGRTLRDLILGHDTELTRLPWVGH
                     RSRSWEPEPLRWLGANAALWTMGSADRAEARTGKPSRRAQVVGKLIGL"
     gene            complement(5794..5875)
                     /locus_tag="VEV43_04845"
     tRNA            complement(5794..5875)
                     /locus_tag="VEV43_04845"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5839..5841),aa:Tyr,seq:gta)
     gene            complement(5917..>6894)
                     /locus_tag="VEV43_04850"
     CDS             complement(5917..>6894)
                     /locus_tag="VEV43_04850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYP22879.1"
                     /translation="DKKKDRKPGEDDRKGADDKKKRKNKKNKDDEILPTPSIPGSFNT
                     EELLATASRLRSLGLSEHEIASKVFAPFIIGGYATWIDTWGAPRYGPGPLVRTHEGQD
                     VFCDMGTPLLASEAGTIEFDDGGLGGLVARLHRSDGSYWYYAHLSDWNTEEFSSGDRV
                     EVGDVIGYCGNTGNALTTPAHVHFGWYQPNGEAKNPMRMLVFWLRQAQGRALGLEART
                     VDRRVRIADRLTFARQFGDAFAPDRSELTVSGGSLWASGSSPATGAFALAESALQEAL
                     ARTTFALDETTTTAFTDLGVGNAVEGEAAPDSRLTDLLSGHDHATESSD"
BASE COUNT          971 a         2455 c         2486 g          982 t
ORIGIN      
        1 gcgcgcctcg tcgcgctcgg ggcggcccgc gagcgcgcgg ctcgcggcgt ggaggggtcg
       61 caggcgctcg gcttcgtcgc gctggcgcag caccgcgcgg tagccgaggt ataggccgag
      121 cagcgggagc gggaacaacg cgacggtcca gcgactcgac aggatcgcgg aggcgaagag
      181 gagcccgagc acgacgctgc cggcgaggtt gatcaacgct ggcgtccaga ctccgtcgag
      241 cgcgctcgtc agccgcgcgt tcccggcgat cgccatcacg atcgcgacga agacgtgggt
      301 caggacggtg aacgtgagca cggcgagcac ggcgactgcc gcgttgcgca gcgtcagcgc
      361 gggcgccggg tcggagagcg cgtaccagac gcccgccgca gccgccgctc cacagccgta
      421 ctgcgcgacg ttgaagatcc ccttgagcca ccccgccttg cggcggtaga cgcgggccgc
      481 ggcgatcgcg agagtgaccg cccagacgag ctcgcccgcg gggagcacga ccaccatcgg
      541 gagcagcaag gcttccgacg cgctcagccc ccagtcgccg cccttgccgt ggaacgtgag
      601 gtcggtgagc tcggtgagag cgaacaggat cgtgaggacc gcgaaggccg cgacctcccg
      661 tcccggcgcc gggtccgtcg tcgagccgag gagcacgacg acgagggccg ctgcgagtgc
      721 ggcgacgtag aggtgaaggg cgcgggacct tagccccacg tcattcccat gccgccgcat
      781 acctcctggt ctgccacgac gtggccttcc aggtcgcgcc cttccacgtc gcgcccttcc
      841 acgtcgcacc gtcccagtcg gcgttcttcc aggtcgcgcc cttccacgtc gcgccgtccc
      901 agaccgtcgc cttccacgtc gcgcccttcc aggtcgcgcc cgcccagtcg ccggacttcc
      961 aggtcgcgcc ggcccacgac tcggcgtccc agccgtcggc cttccaggtc gcgccggccc
     1021 acgggacgag accggccggg ttcgtcacgg agacttcgtc ggggtcggtc tgcgcgacgt
     1081 actcgccctg gaggaagatc tgcccgagag gcgtcacgac ctcgacgccc gcgcttcccc
     1141 ggtcgccctc gaggaggccg agccctgtcg cctgaccgag tccctggttc gccgcggcca
     1201 tgctcgtgct ggtcgcggcg gcgaacgcgt cgaccaggcc cgcgcccgca cgcatgcggt
     1261 cgctctcggc gatgccgcgc gccgtgctcg tgagccgtgc cttcacctgg tcgggcgtca
     1321 ggcccggctc gtcctggagg agctgcgcga cgatcccgct cacggtcgcg gtcgccatgg
     1381 acgtcccggt ccccttgaag tagtggccgc cgatcgcggc ggtcgagccg tagcgctggt
     1441 cgatcgcgct cccgggcgag cgcagcgaca ccgtgtgcac gccgggcgag acgaggtccg
     1501 gcttcgccag tccgttcgca cgcgtggggc cgcgcgacga gaagacgggc acgcggtcgt
     1561 cacccgtcgc catcgatccc tcgtcgtccg acgcgccgac ggtgatgacg taggggtcgt
     1621 cgccaggctt catcacggtc ccggcatccg ggccggagtt gccggcggac acgacgacga
     1681 cgatgcccga cgcccacgcc cgttcgacgg cgtagttgag cggcgagagc cggtcgtcct
     1741 gcgccgagtc cgtgccgagc gagaggttca tcacgcggat gccgtacacg tccttgtggg
     1801 ccacgatcca ctggatgccg gcgaggacag tcgatacgtc ggtcgcaccg tcgaaccccg
     1861 cggccttgac ggagacgatg cgcgcgccgg gggccgcacc ggtgtgcttg ccgtgcgacg
     1921 cgctgccgtc gcctgcgatg agacccgcca tgaacgtgcc gtgaccgaac gtgtccgcgc
     1981 agtgggcctc ggtgccggct tcgtgcgaga ggtcctcgca gtgcacgacc ctcccggcga
     2041 ggtcgggatg cgcggcgtag acgccggtgt ctagcagcgc gactgtcgtg ccggctccgt
     2101 cgatgccgcg cgcccacagg tcgttcgagc gcacgatctt ctggatgcgg tgggcggtcc
     2161 ccgtgtccgg ggcaccgtcg aacgcgatcg ggtcgtcggc ggtgaccgaa cgtacgcgcg
     2221 ggtcggcccg cagctcgtcg aggcgctccg cggggatcct cgccgagacg ccgtcgacga
     2281 tcggcaagac cgcgtcgacg gcgcctcccg aggaggtcac cgcttcggct gcgaagaggg
     2341 ccgatcccgc gccttggacg acgacctcga gcatggagcg tgagccggcg gggccggaga
     2401 cggggagcgt cgcgacgagc agcaacagcg ccccggcgag tgtcccgcgg cggacgctag
     2461 cggtgggcgg cggcatcgtc ttcgagtgca tctgctcctc ctggcgtgcc gccggccccc
     2521 ctagccgcta cgtactatgg ccgacggtct cggcgctgga catagcccct ttggaccaaa
     2581 aagcgcacag cccatgtgga ctagtcccgc agcgcaaaca ggtgccttcg aggaccacgc
     2641 catgcacgcc tgagacaatg cctcgatgag aaccggccgc gactgggagc ccgcattcgc
     2701 gggcgcgacg acgttccaga aggtcccgct cgccttggac gaggaatcgc tgcagggggc
     2761 cgacgtagcg atcgtcggcg cgccgatgga cgagtacgtc acgcaccgtc ccggcgcacg
     2821 cttcgggccg cgtgcgatcc gcaccgcgat cgacgggggc ggccctccga agacgtggca
     2881 catggacctc ggggtcgacc cgttcgcgga gctgcgggtc gtcgaccacg gtgacgccga
     2941 ggtcgttccg ggcgaccccg ccgcgagcca cgtcgccatc tctgctgcgg tcggccgcgt
     3001 cgtggccgcc ggcgcggtgc cggtcgtgct gggaggcgac cactcgatcg cgtaccccga
     3061 cgtagccgcg gttgccgcgt cgctgccgaa gggctcgctc gcggtcgtgc agttcgacac
     3121 gcacacggac acggcgaccg agaactgggg cgtggcgcgt gcgcacggga cgccgttccg
     3181 acacctcgtc gacgaagggg tcctccccgg ggaacggctc gtgcaggtcg gcctgcgcgg
     3241 gtactggccg aaccccgacg agtgggcgtg ggcgcgcgcc gcgggcgtgg gctggcatcg
     3301 gatggaagac gtgatcgatc gcggcataga cgcggtgatc gacgacgtgc tcgagcggat
     3361 cgcgccggcg gagcacctgt tcctgtcggt cgacgtcgac gtgctcgatc ccgccttcgc
     3421 gccggggacg gggacgccgg agccgggcgg catgtcgacg cgcgtgctgc tgcgggcgat
     3481 acggaagctg acggtggcga agggcctggc cgggatggac gtggtcgagg tctcgccgcc
     3541 gtacgaccac gccgacatca cggcgatggc cgcgcaccgc atagtcgtag acgcgctggc
     3601 gggcctggcc gttcaccgcc ggggcggaga cgtgaggccc gagcgtgcct agcgtgcccg
     3661 cgccggggcc ggttcccggc gaggacgacc ttctggaacg cgtcgccggg ctggtccgct
     3721 ctgccggccg cgtcgtcgcg ctcacggggg ccggaatctc taccgagtcc ggcatccccg
     3781 acttccgcgg cccccagggg gtctggacca aggacccgaa ggccgaacgg ctctcgaccc
     3841 tgaaggccta catggaggac ccggagctga gggtcttcgc gtggcggtcc cggatggagc
     3901 acccggcgtg gacggcaacg ccgaacgcgg gccaccttgc gctcgcccag ctcgagcgca
     3961 agggcaagct gcatacgctc gtcactcaga acatcgacgg gctgcatcag aaggcgggca
     4021 cgtcgcccga ccggctcgtc gagatccacg gcacgctgcg cgaggtcatg tgcatgtcct
     4081 gcgacgagcg tgccccgatg gaggccgcgc tggaaagggt gagggcgggt gaggcggatc
     4141 cggcgtgccg cacgtgtgga ggaatcctca agtcggcgac gatctcgttc ggccaggcgc
     4201 tgaacgaagc cgacatgcga cgcgcgcacg aggcggccga gtcgtgcgac ctcttcctcg
     4261 ccatcgggac gtcgctgacc gtctatcccg tcgcgtactt gcccgccgtc gcggcgggcg
     4321 cgggagcgac ggtcgtcgtg atgaacgcgg agccgacgcg ctacgacgcg gcggccgacg
     4381 tcgtggtgcg gacgcccgtg ggtcgcgcgc tgtccgagat agctgcgcgc gtttagaggc
     4441 cgatcagctt cccgactacc tgcgcgcgcc gcgacggctt gcccgtgcgg gcctcggccc
     4501 ggtcggcgct gcccatcgtc cacaaggccg cgttcgcgcc gagccagcgc agcggctccg
     4561 gctcccacga cctcgaacgg tggccgaccc acgggagccg cgtgagctcg gtgtcgtgcc
     4621 cgaggatcag gtcgcgcaac gtcctgccgg cgaggttcga cgtggacacg ccgtcgccga
     4681 cgtagccgcc cgcccacgcg aggccggcgg cgcgatcgaa gccgaccgac gagtaccagt
     4741 cgcgcgggac gccgagcgcg cccccccagc gatgcgtgat gcgcgcgccg cgggcgtcgg
     4801 gccagagctc gtggaggaca cgttcgagct cggcgaacac cttcggctcg tcgtcgaagc
     4861 tgtcggagat gcgcgacccg tagtggtagg gcgcgccgcg gcccccgaac gcgatccggt
     4921 cgtccgcggt gcgttgtgcg tagacgagca ggtgacgccc gtcggtgatc gtctcggcac
     4981 ccgcccaccc gacgtgttcc cagaacgact cggacagagg ctcggtgacg atcatcagcg
     5041 agtagagcgg gacgacgcgc cgccgcatcc gcggcagcgt caccgagtac ccctcggtag
     5101 cgcgcacgac gacgtctgcg gtcagtgacg ctccggggac gtcgatccgg ccccgctcga
     5161 tccccagcgc gggggtcctc tcgcagatcg acacacccat gccctcgacg acgcgggcaa
     5221 ggccgcgcac gagccgcgcc gggtgcacgc gcgcacagtg cggtgtgaac gcggccccta
     5281 agcagcccgg gacccggatc cgctccgcca cctcggcctc tcccagccag cgcgcgtcgg
     5341 acgacgcggc ctcgtcgcgc acgcgaggca cgtgagccgg cgaggtcgcg agcgtcagcg
     5401 tcccgccctt gtgccagtgc gcgtcgatgc cttccgcgtc cacgacccgg ccgacctcgt
     5461 cgaccgtcgc cgcgaggggg ccgtgcatgt ccttcgcgct ccgggctcct gcgaacagag
     5521 cggagcacca cccgccgttg cggcccgacg cgccgaagcc tgcgatctcc ttctcgacga
     5581 tcgccacgcg cagcgagggg tccgcctgct tgtggtagtc cgcggtcaac aaccccgtgt
     5641 accccgcgcc gacgatcgct acgtcgaccg tctcggggct tcgcaacgac gggcgcggcg
     5701 tgaggtcgtc gccgcaggtg tcgagccaga acgagtacga gcgatagtcc ttcacgtctc
     5761 tcctcgaccg ctcgcccagc cgacccgtgg cagtggcggg accaggattc gaacctggga
     5821 aggacaagcc aaccggttta cagccggtcc tctttggcca cttgagtatc ccgccgagtt
     5881 gctgcgttgc gaagatatcg caacggacgg tgcgccctag tcgctcgact ccgtcgcgtg
     5941 gtcgtggccg gagagcaggt cggtcagacg agagtccggc gcggcctctc cctcgacggc
     6001 gttcccgacg ccgaggtcgg tgaacgcggt ggtcgtcgtc tcgtcgagcg cgaacgtcgt
     6061 gcgtgccagc gcctcctgca gcgccgactc ggccagcgcg aacgcgccgg tcgccggcga
     6121 agagcccgac gcccacaacg agcctccgga caccgtcagc tccgagcggt ccggtgcgaa
     6181 cgcgtcgccg aactggcggg cgaacgtgag gcggtcggcg atcctgaccc gcctgtcgac
     6241 cgtgcgggcc tcgagaccga gtgcccgccc ctgcgcctgg cgcagccaga agacgagcat
     6301 gcgcatcggg ttcttcgcct cgccgttggg ctggtaccag ccgaagtgga cgtgtgccgg
     6361 cgtcgtcagg gcgttgcccg tgttgccgca gtagccgatg acgtcgccga cctcgacccg
     6421 gtcgccggag gagaactcct cggtgttcca gtcggagagg tgcgcgtagt accagtagga
     6481 gccgtcggaa cggtggagcc gcgcgaccag gcccccgaga ccgccgtcgt cgaactcgat
     6541 cgtcccggcc tcggacgcga gcagcggcgt gcccatgtcg cagaagacgt cctggccctc
     6601 gtgcgtccgc acgagcgggc ccgggccgta ccggggcgcg ccccaggtgt cgatccacgt
     6661 cgcgtagccg ccgatgatga acggggcgaa gaccttgctc gcgatctcgt gctccgagag
     6721 gccgaggctg cgcagccgcg aggccgtcgc gaggagctcc tcggtgttga aggagcccgg
     6781 gatcgacggg gtcgggagga tctcgtcgtc tttgttcttc ttgttcttgc gcttcttctt
     6841 gtcgtcggcg cccttgcggt cgtcctcgcc gggcttgcgg tccttcttct tgtc
//
LOCUS       DASQDO010000165         5110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_629095, whole genome shotgun sequence.
ACCESSION   DASQDO010000165 DASQDO010000000
VERSION     DASQDO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629095"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VEV43_04855"
     CDS             complement(<1..333)
                     /locus_tag="VEV43_04855"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP22880.1"
                     /translation="MRRAGVAVLAVAVLATAVLGVMLLARERGGPRHRDVTLAGDVPA
                     TFYLPDPPLDGAPAVVVAHGYSGDRATMSSLARSIALAGYGVLAIDFRGHGANPNAVE
                     EGARTDDIE"
     gene            348..719
                     /locus_tag="VEV43_04860"
     CDS             348..719
                     /locus_tag="VEV43_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22881.1"
                     /translation="MARRKKSELRSQARLARAQGLFNVVGGAWPLVWLRSFEWIFGRK
                     RDVYLQKTSGLFFLASGIALLLAEETEDGMAAARRIGVGAASVYLVIDLIYVPKRDIP
                     STYMLDALMEAGWLYGWARRS"
     gene            complement(728..1876)
                     /locus_tag="VEV43_04865"
     CDS             complement(728..1876)
                     /locus_tag="VEV43_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYP22882.1"
                     /translation="MRARRLDEVPGFGIDKVAAAAGDDPEVLRLENLDTDLPPPPGAV
                     EATQAAVVTPEGNSWLPFSGRADLKEAVADHVERRSGVRYDPASEVVITATDGSGMLD
                     ALLATTDPGDEVVLTDPTYAGMINRVRLAGAEPRFARCEVVDGEWRLDLDHLRSSVTD
                     RTKALFLQNATFPSGMVFTRAEWEAIAELVIERSLHLVYWGFMESVLFDGRDLVHPVS
                     LPGMRERTIVLGGVAIEYRMIGWRVGWALAPREAADAVGVCHVYNGLVASGFAQTGAV
                     AALRSDPSDLVSSTAELQRRRDTVMAQLEGLPAVSASGGWALLLDTEAIGIPPAEASA
                     RLLEQKVAATPMTVWGETVAPRYVRFVFSREPVARLELLGERVRAALT"
     gene            complement(1976..2776)
                     /locus_tag="VEV43_04870"
     CDS             complement(1976..2776)
                     /locus_tag="VEV43_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP22883.1"
                     /translation="MSQLALALGDGAMVAKRNLIKIKRVPEVLIWTTMSPIMFVLLFA
                     YVFGSAIDVQGVSYREFLIAGVFAQTIVFGSTFTGAGLAEDMTKGIIDRFRSLPMARS
                     AVLVGRTASDIFYNSTSLVVMSLAGLIVGWRVRTSIVEAIGGFLLLLLFSYAISWIMA
                     YVGLKVSSVEVVNNAAFMFIMPMTFISNAFVPVDGLPGPLKAFADWNPVSSVAQGARE
                     AFGNTGGMAMPDAWSLQNPVLYTLIWVALILAVFVPLSVRQYKKAAAR"
     gene            complement(2773..3783)
                     /locus_tag="VEV43_04875"
     CDS             complement(2773..3783)
                     /locus_tag="VEV43_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP22884.1"
                     /translation="MADMIRTEGLVKRYGDVVALNGLDLSVPAGTVLGLLGPNGAGKT
                     TAVRILTTLLEPDEGKVEVAGLDVRAEPGKVRERIGLSGQNAAVDEHLTGFENLEMVG
                     RFYHLGKQRSRERANQLLEEFDLVDAGKRPAKTYSGGMRRRLDLAAALVATPDVIFLD
                     EPTTGLDPRSRTQMWETIQGLVRGGATVLLTTQYMEEADRLADDIVVIDRGRKIAHGT
                     ADHLKTEIGGERIEVVLADAADIPAGRSVLAGLAIGEIQVDEQARRLTAAVRGGVDNL
                     KRVLDEITERQVAVVDVGLRRPTLDDVFLSLTGHSAEEVADGGESTADGTPEIREKEA
                     AR"
     gene            complement(3800..>5110)
                     /locus_tag="VEV43_04880"
     CDS             complement(3800..>5110)
                     /locus_tag="VEV43_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP22885.1"
                     /translation="SSVIAAFSTSAEMLIAARALLGIAGATVAPSTLSLIRNMFHDPH
                     QRMTAIGVWGTSFAAGGAVGPLVGGVLLEAFWWGSVFLINVPVMALLLVLGPRLLPEF
                     KDPTAGRIDLASALLSLAAVLPVIYGLKHVAQDGASWGGFAPVIAGLAFGALFVRRQR
                     RLDDPLIDLKLFRFPAFSVSVASNTLTGFIAFGTFLFIAQYLQLVVGLSPLEAGMWSL
                     PSSVAVVFGSMMAPRVSRVVRPAFGVAGGLLLAAAGLALLTQVDGTSGLPIVVTGGVL
                     FSLGLGPVFILATDLIVGTAPAERAGAASAISETGAELGGALGIALLGSIGTAVYRTG
                     VTDALGTGVPDAARDTLGGALAVAERMPGDSARELVAVAHDAFTQGLHLSATVGAAVA
                     VGVALLVATLLRNVRGSGHEDVTPPTEDREADGLVALQPVCAEA"
BASE COUNT          812 a         1863 c         1729 g          706 t
ORIGIN      
        1 ctcgatgtcg tcggtccggg ccccttcctc gaccgcgttc gggttcgcgc cgtggccgcg
       61 gaagtcgatg gcgaggaccc cgtatcccgc gagggcgatg ctgcgcgcga gcgagctcat
      121 cgtcgcgcgg tcacccgagt agccgtgggc gacgacaacg gccggcgcgc cgtccagtgg
      181 tgggtcgggg aggtagaagg tcgccgggac gtcacccgcc agcgtgacgt cgcgatgacg
      241 agggccccca cgttcccgtg cgaggagcat cacccccagc accgccgtcg cgaggaccgc
      301 gaccgccagc accgccacgc ccgcccgccg cattacgctg acgctacatg gcgcgccgga
      361 agaagagcga gctcaggtcc caggcccggc tcgcgcgggc gcagggcctg ttcaacgtcg
      421 tcgggggcgc gtggccgctc gtgtggctgc gctcgttcga gtggatcttc gggcgcaagc
      481 gcgacgtgta tctccagaag acgtccgggc tcttcttcct cgcgtcgggc atcgcgctgc
      541 tgctggccga ggagaccgag gacggcatgg cggccgcacg ccgcataggt gtcggagcgg
      601 ccagcgtgta tctcgtgatc gacctgatct acgtcccgaa gcgcgacatc ccgtcgacgt
      661 acatgctcga cgcgttgatg gaggccggct ggctctacgg atgggcccgc cggagctgat
      721 cagccgctca cgtcagggca gcgcgcacac gctcgcccag cagctccagc cgggcgacgg
      781 gctcgcggct gaacacgaac cggacgtagc gcggcgccac cgtctcgccc cacaccgtca
      841 tcggcgtcgc cgcgaccttc tgctcgagca gccgcgccga cgcctccgcc ggcgggatcc
      901 ctatggcttc cgtgtcgagc aggagcgccc acccgccgga cgcgctcacc gcagggagcc
      961 cttcgagctg cgccatcacg gtgtcccggc gccgttgtag ctcggcggtc gaggagacga
     1021 ggtccgaggg gtccgaccgc aacgcggcca cggcccccgt ctgcgcgaac ccactcgcga
     1081 ccagcccgtt gtagacgtgg cacacgccga cggcgtcggc ggcctcgcgc ggggcgagcg
     1141 cccacccgac gcgccagccg atcatccggt actcgatcgc gacgcccccg agcacgatcg
     1201 tccgctcgcg catgcccggc aacgagaccg ggtggacgag gtcgcggccg tcgaacagca
     1261 cgctctccat gaagccccag tacacgaggt gcagagaacg ttcgatcacc agctcggcga
     1321 tcgcctccca ctccgcccgc gtgaacacca tccccgacgg gaacgtcgcg ttctgcagga
     1381 acagcgcctt cgtgcggtcc gtcacgctcg accgcagatg gtcgaggtcg agccgccact
     1441 cgccgtcgac gacctcgcac cgggcgaacc tcggctcggc ccccgcgagc cggacccggt
     1501 tgatcatccc cgcgtaagtc ggatcggtca gcacgacctc gtcgccgggg tcggtcgtcg
     1561 cgagcagggc gtcgagcatc ccgctcccgt cggtcgcggt gatcacgacc tcggaagccg
     1621 ggtcgtaccg gactccgctg cgccgctcga cgtggtcggc cacggcctcc ttcaggtcgg
     1681 cccggccgct gaacgggagc cagctgttgc cctcgggcgt caccacggcc gcctgcgtgg
     1741 cctcgacggc accgggcggc ggcgggagat cggtgtcgag gttctcgagc cgcaagacct
     1801 cgggatcgtc tccggcagcc gcggcgacct tgtcgatgcc gaagccgggc acctcgtcga
     1861 gccgtcgagc gcgcatgggg ctcctttcgg gagtgggggc cccatgctcg cgccgggccg
     1921 ggcgcgggcg cgacgcggcc cccgggatgc aaccccgggg gccgcgaagg atcggctagc
     1981 gggctgcggc cttcttgtac tggcgcaccg acagtgggac gaataccgcc aggatcagcg
     2041 ccacccagat cagcgtgtac aggaccgggt tctgtaacga ccacgcgtcc ggcatcgcca
     2101 tgcccccggt attcccgaag gcttctcggg ccccctgcgc gaccgacgac accgggttcc
     2161 agtctgcgaa agccttcagc gggcccggaa ggccgtcgac gggcacgaac gcgttcgaga
     2221 tgaaggtcat cggcatgatg aacatgaacg ccgcgttgtt gacgacctcg acgctcgaca
     2281 ccttcaggcc gacgtacgcc atgatccacg agatcgcgta cgagaacagc agcaggagga
     2341 ggaacccgcc gatcgcctcg acgatcgagg tccgcacccg ccacccgacg atgagcccgg
     2401 ccagcgacat gacgaccagc gacgtcgagt tgtagaagat gtcgctcgcc gtgcgcccca
     2461 cgagcactgc ggaccgcgcc atcggcagag aacggaaccg gtcgatgatc cccttcgtca
     2521 tgtcctcggc gagaccggcc cccgtgaacg tcgagccgaa cacgatcgtc tgggcaaaga
     2581 ctcccgcgat gaggaactcg cggtagctca cgccctggac gtcgatcgcg cttccgaaca
     2641 cgtacgcgaa cagcagcacg aacatgatcg gcgacatcgt cgtccagatc aggacttcgg
     2701 gcacgcgctt gatcttgatc aggttgcgct tcgcgaccat cgcgccgtcg cccagcgcga
     2761 gagcaagctg gctcatcgtg cggcctcctt ctcgcggatc tcgggtgtgc cgtccgcggt
     2821 ggactcgcca ccgtcggcca cctcttcggc ggagtgtccc gtcaacgaca ggaagacgtc
     2881 gtccagcgtc gggcgcctca ggcccacgtc gacgaccgcg acctggcgct cggtgatctc
     2941 gtcgaggacg cgcttgaggt tgtcgacgcc tccacggacc gcggccgtca gcctgcgggc
     3001 ctgctcgtcg acctggatct cgccgatcgc caggcccgcg agcaccgaac gtcccgccgg
     3061 tatgtcggcg gcgtcggcga gcacgacctc gatccgctcg ccgccgatct cggtcttgag
     3121 atggtccgcc gtcccgtgcg cgatcttgcg gccgcggtcg atgacgacga tgtcgtcggc
     3181 gagtcggtcg gcctcctcca tgtactgcgt cgtcaggagg acggtcgcgc caccccggac
     3241 gaggccctgg atcgtctccc acatctgcgt ccggctacgc gggtcgagcc ccgtcgtcgg
     3301 ctcgtcgagg aagatcacgt ccggggtcgc gaccagcgcg gccgcgaggt cgagcctgcg
     3361 ccgcatcccg cccgagtacg tcttcgcagg acgcttgccc gcgtcgacga ggtcgaactc
     3421 ttcgagcagc tggttcgcgc gctccctaga gcgctgcttc cccaggtggt agaagcgacc
     3481 gaccatctcg aggttctcga agcccgtgag gtgctcgtcg accgccgcgt tctggccgga
     3541 gagcccgatg cgctcgcgca ccttgccggg ttccgcccgc acgtccagcc ccgcgacctc
     3601 cacctttccc tcgtcgggct cgagcagtgt cgtgaggatc cgcaccgccg tcgtcttgcc
     3661 ggcgccgttc gggccgagaa gcccgaggac ggtcccggcg ggaacggaga ggtcgagccc
     3721 gttcagagcc acgacgtcgc cgtagcgctt gacgaggcct tccgtcctga tcatgtctgc
     3781 catccgtttc acctttcctt caagcttccg cgcagacggg ctggagggcg acgagcccgt
     3841 cggcttccct atcttccgtg gggggtgtga catcttcgtg gccggagccc cgcacgttgc
     3901 ggagcagcgt cgccactagg agagcgacgc ccacggcaac ggctgccccg accgtagccg
     3961 agagatgcaa accctgtgtg aacgcgtcgt gggcgacggc gacgagctcg cgggccgagt
     4021 cgcccggcat ccgctccgcg accgccaggg cccctccgag cgtgtcgcgc gccgcgtcgg
     4081 gcacgcccgt cccgagcgcg tccgtcaccc cggtccggta gacggccgtg ccgatgctcc
     4141 cgagcagcgc gatcccgagg gcgccgccga gctcggcccc cgtctccgag atcgccgaag
     4201 cggcccccgc ccgctcggcc ggagccgtcc cgacgatgag atccgtggcg aggatgaata
     4261 ccgggccgag cccgagcgag aagaggactc cgccggtcac cacgatcgga agacccgagg
     4321 tcccgtcgac ctgcgtgagc aacgcgaggc cggccgccgc cagcaggagc ccaccggcca
     4381 cgccgaacgc gggccggacg acccgtgaga cccgcggcgc catcatcgag ccgaacacga
     4441 ccgccaccga cgagggcagc gaccacatcc cggcctccag cggcgagagc ccgacgacga
     4501 gctgcaggta ctgggcgatg aacaggaagg tcccgaaggc tatgaacccg gtgagcgtgt
     4561 tgctcgctac cgagacgctg aaggcgggga agcggaacag cttcaggtcg atcaaggggt
     4621 cgtcgagccg cctctgccgg cgcacgaaca gcgcaccgaa cgcgagcccg gcgatcaccg
     4681 gcgcgaaccc gccccaactc gccccgtcct gagcgacgtg cttcaggccg tagatcacgg
     4741 gaagcaccgc cgcgagcgag agcagcgcgc tcgccaggtc gatgcggccc gcggtcgggt
     4801 ccttgaactc ggggaggaga cgggggccga gcacgagcag caacgccatc accggcacgt
     4861 tgatcaggaa cacggagccc caccagaacg cctccagcag gacaccgccg acgagggggc
     4921 cgacggcgcc gccggcggcg aagctcgtcc cccacacgcc gatcgcagtc atgcgctggt
     4981 gcgggtcgtg gaacatgttc ctgatgagcg aaagcgtcga cggggcaacg gtcgcgcccg
     5041 ctatgcccag aagagcccgc gccgcgatca gcatctccgc gctcgtggag aacgccgcga
     5101 tgacggacga
//
LOCUS       DASQDO010000166         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_751480, whole genome shotgun sequence.
ACCESSION   DASQDO010000166 DASQDO010000000
VERSION     DASQDO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751480"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VEV43_04885"
     CDS             complement(<1..763)
                     /locus_tag="VEV43_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HYP22886.1"
                     /translation="MTITPDDLISKTFNMTLRRGYDKEEVDAFLIELAEDYRTALQRA
                     GSGGGAAAPSGADPFSAMGEEVTAVLRTAKESAEALRRKTEEEAEGIRRRAADKAIEV
                     RKQAQDQATSMVDSAKKKAEQLTQDADRYAQETRSKADREASEARNQAERYAQDTRNK
                     ADGEASYLRTEADRYAKETRAKADQEYTEAKTKAQAEQKAILDEATRRHEALLAHERE
                     LRDRIAGVEKALQAVQAELGGAAPAAAAPKRSGGKG"
     gene            complement(867..>1530)
                     /locus_tag="VEV43_04890"
     CDS             complement(867..>1530)
                     /locus_tag="VEV43_04890"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HYP22887.1"
                     /translation="AMADETPTSKKLLEKQQRRAAEEERRREQQKAARKRNLVTLVIA
                     LVVIVLVATLVIQNRDESGGNLPSVGRGEAGCTEIEEHDIEGQEHVDVTPQYQTNPPT
                     SGNHLQTPASPGFFDEAVDPGAVVHNMEHGQIIFWYDPEAPPDVIDAIEAAVDQQFDA
                     SLAIPWDEIESPYNFAMTAWGASQHCREVSQSVVDAFREEYQGRGPEVIPGIDTYEAP
                     EA"
BASE COUNT          168 a          535 c          523 g          304 t
ORIGIN      
        1 tgcccttccc cccggaacgc ttcggagcgg cagcggcggg tgcggccccg ccgagctcgg
       61 cctggaccgc ctggagcgcc ttctcgacgc cggcgatcct gtcgcgcagc tcgcgctcgt
      121 gggcgagcag cgcctcgtga cgcctcgtcg cctcgtcgag gatcgccttc tgctcggcct
      181 gggccttcgt cttggcctcg gtgtactcct ggtcggcctt ggcccgcgtc tccttcgcgt
      241 agcggtcggc ctcggtccgg aggtaggacg cctcgccgtc ggccttgttg cgggtgtcct
      301 gcgcgtagcg ttcggcctgg ttgcgcgcct cggacgcctc gcggtcggcc ttcgagcggg
      361 tctcctgcgc gtagcggtcc gcgtcctgcg tgagctgctc ggccttcttc ttcgcggagt
      421 cgaccatcga ggtggcctgg tcctgggcct gcttgcgcac ctcgatcgcc ttgtcggcgg
      481 cgcggcggcg gatgccctcg gcctcttcct cggtcttgcg acgcagcgcc tcggcggact
      541 ccttcgcggt ccggaggacg gccgtgacct cctcgcccat cgcggagaaa ggatccgcgc
      601 cggacggagc cgcagccccg ccgcccgaac ccgcgcgctg gagggcggtg cggtagtcct
      661 cggccagctc gatcaggaaa gcgtcgacct cctctttgtc ataacccctg cgcagggtca
      721 tgttgaaggt cttgcttatc aagtcgtccg gcgtgatcgt catgaaccgc tacccccgct
      781 gcgttggtcg aagcgacgat tttcagtata tgtggcgggg tcttcggacc cccggctgaa
      841 acgtcgcgat ctacgcgccg gccgcgctat gcctcgggcg cttcgtacgt gtcgatgccc
      901 gggatgacct ccggccccct gccctggtac tcctcgcgga aggcgtccac gaccgactgc
      961 gagacctcgc ggcaatgctg cgaggctccc caggcggtca tcgcgaagtt gtacggggac
     1021 tcgatctcgt cccacgggat cgcgagggat gcgtcgaact gctggtcgac ggccgcctcg
     1081 atcgcgtcga tcacgtccgg gggcgcctcc gggtcgtacc agaagatgat ctggccgtgc
     1141 tccatgttgt gcacgacggc acccgggtcg accgcctcgt cgaagaagcc gggcgacgcg
     1201 ggggtctgta ggtggttgcc ggaggtcggc gggttggtct ggtactgcgg cgtgacgtcg
     1261 acgtgctcct gcccctcgat gtcgtgctcc tcgatctcgg tgcacccggc ctcgccacga
     1321 ccgacgctcg gcaggttgcc gccactctcg tcgcggttct ggatgacgag cgtggcgacg
     1381 agcacgatga ctacgagcgc gatcacgagc gtcacgaggt tgcgcttgcg cgcggccttc
     1441 tgctgctcgc ggcgccgctc ttcctcggcg gccctccgct gctgtttttc gagaagcttc
     1501 ttgctggtgg gggtctcgtc agccatcgcc
//
LOCUS       DASQDO010000167         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_322820, whole genome shotgun sequence.
ACCESSION   DASQDO010000167 DASQDO010000000
VERSION     DASQDO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322820"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VEV43_04895"
     CDS             complement(<1..783)
                     /locus_tag="VEV43_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYP22888.1"
                     /translation="MEFSVPEVRLGGIAPEVVLCATALFMLVVHAVLGARLPRWTLAA
                     TSTIALGAAGWFAYDAWDVNELQLEGMVATDGFAAFVKISLAVFAVLTVWLGRAHISS
                     DELDESEFYALVLFATAGMMLMAGAADLIMMFLALETFSIALYVLVAFRRRSLASQES
                     SMKYFLLGAFSSAFFLLGIALVYGAVGSTNLYGTGSQPGIVEFLRTTPTSDVTLLFLA
                     AGLLIVGLGFKVAAVPFHMWTPDAYQGAPSTVTGFMAAASKLA"
     gene            complement(783..2294)
                     /locus_tag="VEV43_04900"
     CDS             complement(783..2294)
                     /locus_tag="VEV43_04900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYP22889.1"
                     /translation="MTDLNLLDLVIFLPVIGAALLGLIPKGRDDLFRFAALAVTLAAF
                     VLSLGVLAQFDAANADFQLGSSLVWIEDWGVGYETGIDGVSLWMVMLTTFLMPLAVLA
                     SWTVTRQVRAYFVFLLALETGMLGVFCALDMFLFYLFWEATLVPMYFLIGMWGYGRRI
                     YAAMKFFLFTLVGSLLMLAAILFLYFQGAEPTFDYRALLGTPLSLDVQRLLFLGFFAS
                     FAVKVPLVPLHTWLPDAHTEAPTAGSVLLAGVLLKMGAYGLIRFGIPLFPDAARELVP
                     LLIALAIAGIVYGAVVAAVQRDLKRLIAYSSVAHLGFVVLGIFVGTVEGMSGGIIQMV
                     SHGISTGALFMLVGILYERRHTRLIADFGGLAKTVPVLAGVFLLVTMSSIGLPGLNGF
                     VGEFSVLVGTFLSYRWWVVPAAFGVVLAAVYLLWAYQRVFYGPVTSEENEKISDLRPR
                     ELVMLAPLLVLIVAMGVYPEPFLERINPSAEKVVRQLNDSSVLPPGDDVAGGD"
     gene            complement(2291..4207)
                     /gene="nuoL"
                     /locus_tag="VEV43_04905"
     CDS             complement(2291..4207)
                     /gene="nuoL"
                     /locus_tag="VEV43_04905"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYP22890.1"
                     /translation="MTHEPTFGDLAPFVIAAPLAVSVLLLFFGKRIGRLAGPLASAAI
                     GLSFAMGCVMFFGLLGEEAHGEGHNAREQIVHLFDWIRTGSLNVGADLLVDQLSVIMV
                     LVVSGVGTLIHVYSIGYMHDDPRYPRFFAYLNLFAASMLLLVLADNLVLMFVGWVGVG
                     LCSYLLIGFWFEKRSAAAAANKAFIVNRIGDFSFLLGGFLLFSVTGAFTFGAINHGAG
                     EMSAGIATIAALLLFGGAAGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMV
                     ARLSPVFAAGDALVVVGWIGALTALWAAVMAAAEYDIKRVLAYSTISQLGYMFLAHGV
                     GAYSAGVFHLVTHAFFKALMFLGAGAVMHALGGETDMRKMGGLRKRMPIVGLTFLAGW
                     LAISGIVPFAGFFSKDAILASAWAEEQYVLWAIGLGTALLTAFYMSRLYFRVFEGKDR
                     VPEGAHPHDAPPTMAAALVPLAVLSLLGGAINLPGQLTLEHFLEPVVGASHVPEGLTA
                     YLLAGIALLGSVVGIATARVLYLSRAGSLVRRKLEARLGPLVTAARNKFYVDEIYDRT
                     VVWPGKGIALLSANVVDRRVFDGIVNGTGGTVARFSEGLRRLQTGYVRNYAVLFLLGV
                     VAVFTVLVLRAVGA"
     gene            complement(4212..4520)
                     /gene="nuoK"
                     /locus_tag="VEV43_04910"
     CDS             complement(4212..4520)
                     /gene="nuoK"
                     /locus_tag="VEV43_04910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYP22891.1"
                     /translation="MNGVDPGYYLVLAALMFTIGVVGVLVRRNAIVIFMFVELMLNAV
                     NLVFVTFARMHGEVAGQVLVFFVLVVAAAEVVVGLAIILLIARRRGTANVDEANLLRW
                     "
     gene            complement(4517..>5017)
                     /locus_tag="VEV43_04915"
     CDS             complement(4517..>5017)
                     /locus_tag="VEV43_04915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYP22892.1"
                     /translation="MLFAPNAVHVALFLVGSQVALAVAFLLQGAFFVAIVQIIVYAGA
                     IMVLFLFVIMLLGVDKREALMEPLPGQRPLALGLGALLAAQVLYVVLRGVDFVAGGGA
                     GGPEALEAVNRDPGNVKALAQILFSRYLLPFEVTSILLVVAIVGVMVLARRMGETGSR
                     RVEEPA"
BASE COUNT          988 a         1729 c         1716 g          584 t
ORIGIN      
        1 ggcgagcttg gacgccgccg ccatgaagcc cgtgacggtc gacggggctc cctggtacgc
       61 gtccggcgtc cacatgtgga acggcaccgc cgcgaccttg aagccgagcc ccacgatcag
      121 caggcctgcc gccaggaaca gcagcgtgac gtcggacgtc ggcgtcgtcc gcaggaactc
      181 gacgatgccg ggctggctgc cggtcccgta caggttcgtc gagccgaccg cgccgtagac
      241 gagagcgatc ccgagcagga agaacgccga cgagaacgcg ccgagcagga agtacttcat
      301 cgacgactcc tgcgacgcca gcgaccgccg ccggaacgca acgagcacgt acagcgcgat
      361 cgagaacgtc tcgagcgcga ggaacatcat gatgaggtcg gcggcccccg ccatcagcat
      421 catccccgcg gtggcgaaga gcacgagcgc gtagaactcg gactcgtcca gctcgtcgct
      481 ggagatgtgg gcccgcccga gccacaccgt cagcaccgcg aacaccgcga gcgagatctt
      541 gacgaaggcg gcgaagccgt cggtggcgac catgccctcg agctggagct cgttcacgtc
      601 ccacgcgtcg tacgcgaacc agcctgcggc ccccagcgcg atcgtcgagg tcgcggcgag
      661 cgtccacctc ggcagccgcg ctcccaggac cgcgtgaacg acgagcatga acagagccgt
      721 ggcgcacagc acgacctcgg gcgcgatccc gccgagcctg acctcgggca ccgagaactc
      781 catcagtcac cccccgcgac gtcgtcaccg ggcgggagga ccgaggagtc gttcagttgg
      841 cgcacgacct tctcggcaga agggttgatc cgctcgagga acggctccgg gtagacgccc
      901 atcgccacga tcagcacgag caggggcgcg agcatcacga gctcgcgcgg gcgcaggtcg
      961 ctgatcttct cgttctcctc cgacgtcacc gggccgtaga agacacgctg gtacgcccac
     1021 agcaggtaga cggcagcaag gacgacgccg aacgccgccg ggacgaccca ccaccggtac
     1081 gagaggaacg tcccgacgag gaccgagaac tcgccgacga agccgttgag gcccggcagc
     1141 ccgatcgacg acatcgtcac tagcaggaag accccggcga gcaccggcac ggtcttggcc
     1201 aggcccccga agtccgcgat cagcctcgtg tgccgccgct cgtagaggat gccgacgagc
     1261 atgaacaggg cgccggtcga gatgccgtgg ctgaccatct ggatgatccc gccgctcatc
     1321 ccctcgacgg tcccgacgaa gatcccgagc acgacgaagc cgaggtgcgc gaccgaggag
     1381 tacgcgatca gccgcttcag gtcgcgttgc accgccgcga cgacggcccc gtagacgatc
     1441 ccggcgatcg cgagcgcgat caagagcggc acgagctcgc gcgctgcgtc cgggaacagc
     1501 gggatgccga agcggatgag cccgtaggcc cccatcttca gcagcactcc ggccagcaat
     1561 accgatccgg cggtgggggc ctcggtgtgg gcgtcgggca gccacgtgtg cagcggcacg
     1621 agcgggacct tgaccgcgaa cgaggcgaag aagccgagga acaacagccg ctggacgtcg
     1681 agcgacagcg gcgtcccgag gagcgcgcgg tagtcgaacg tcggctcggc cccctggaag
     1741 tacaggaaca ggatcgccgc gagcatcagc aacgagccga cgagcgtgaa caggaagaac
     1801 ttcatcgccg cgtagatccg gcggccgtag ccccacatcc cgatgaggaa gtacatcggg
     1861 acgagcgtcg cctcccagaa cagatagaag aggaacatgt cgagcgcgca gaacacgccg
     1921 agcatcccgg tctcgagcgc gagcaggaag acgaagtacg cgcgcacctg ccgggtgaca
     1981 gtccacgacg ccagcaccgc gagcggcatc aggaacgtcg tcagcatgac catccacagg
     2041 ctcacgccgt cgatgccggt ctcgtagccc acgccccagt cctcgatcca cacgagcgac
     2101 gacccgagct ggaagtcggc gttcgccgcg tcgaactggg cgaggacccc cagcgacagc
     2161 acgaacgcgg cgagcgtgac ggcgagagcg gcgaaccgga agaggtcgtc gcgtcccttc
     2221 gggatgagcc cgagcagcgc tgcgccgatc accggaagga agatgacgag gtcgagcagg
     2281 ttcaggtcgg tcacgcgccc accgcccgca ggacgagcac ggtgaacacc gcgacgacgc
     2341 cgagcaggaa caagaccgcg tagttgcgga cgtaacccgt ctggaggcgc cggagcccct
     2401 cggagaagcg cgcgacggtc ccaccggtcc cgttgacaat cccgtcgaag acgcggcggt
     2461 cgacgacgtt cgcggacagc agcgcgatcc ccttgccggg ccacaccacc gtgcggtcgt
     2521 agatctcgtc gacgtagaac ttgttgcggg ccgccgtgac gagggggccg agacgcgcct
     2581 cgagcttgcg gcgcaccagc gacccggcgc ggctcaggta gagcacgcga gcggtcgcga
     2641 tgccgaccac gctgccgagc agcgcgatcc ccgccagcag gtaggcggtg aggccttcgg
     2701 gcacgtggct cgcgccgacg accggctcga ggaagtgctc gagcgtcagc tggcccggca
     2761 ggttgatggc tccgccgagc agagacagga ccgccagggg gacgagggcc gcggccatcg
     2821 tcgggggcgc gtcgtgcggg tgcgcgcctt ccggaaccct gtccttgccc tcgaagaccc
     2881 tgaagtacag gcgggacatg tagaacgcgg tcagcagcgc ggtgccgagg ccgatcgccc
     2941 acagcacgta ctgctcctcg gcccacgccg acgcgaggat cgcgtccttc gagaagaacc
     3001 cggcgaacgg cacgatgcct gagatcgcga gccacccggc gaggaacgtg agcccgacga
     3061 tcggcatgcg cttgcgcagc ccgcccatct tccgcatgtc ggtctcgccg ccgagggcgt
     3121 gcatgaccgc tccggccccc aggaacatca gagccttgaa gaacgcgtgg gtgacgaggt
     3181 ggaagacgcc ggcggaatac gcgccgacgc cgtgcgcgag gaacatgtag ccgagctggc
     3241 tgatcgtcga gtaggccagc acgcgtttga tgtcgtactc ggccgcggcc atcacggccg
     3301 cccacagggc ggtgagcgcg ccgatccagc cgacgacgac gagcgcgtcg ccggccgcga
     3361 agacggggct caagcgggcg accatgtaca cgccggcggt gaccatcgtc gccgcgtgga
     3421 tcagcgcgga gaccggagtg ggcccctcca tggcgtcggg gagccacacg tacagcggga
     3481 tctgagcgct cttgcccgct gccccgccga acagcagcag cgcggcgatc gtggcgatcc
     3541 cggcgctcat ctcacccgcg ccgtggttga tcgcgccgaa cgtgaacgcc ccggtgaccg
     3601 agaacagcag gaacccgccg agcaggaagg agaagtcgcc gatccggttg acgatgaagg
     3661 ccttgttcgc ggccgccgcg gccgagcgct tctcgaacca gaagccgatc agcaggtacg
     3721 agcacagtcc gacgcccacc cagccgacga acatcagcac gaggttgtcg gcgagcacga
     3781 gcaggagcat cgacgcggcg aacaggttga ggtaggcgaa gaacctcggg tagcgcgggt
     3841 cgtcgtgcat gtagccgatc gagtagacgt ggatcagcgt cccgacgccg ctcacgacga
     3901 gcaccatgat caccgagagc tggtcgacga gcaggtccgc gccgacgttc aggctgccgg
     3961 tccggatcca gtcgaagagg tggacgatct gctcgcgggc gttgtggccc tcgccgtgcg
     4021 cctcctcgcc gagcagcccg aagaacatca cgcagcccat cgcgaacgag aggccgatgg
     4081 ccgccgacgc gagcggtcct gctagccggc cgatccgctt gccgaagaag agcaacagaa
     4141 ccgacacggc caggggggcc gcgatcacga agggggccag gtcgccgaac gtgggctcgt
     4201 gcgtcacggc gtcaccaccg cagcaggttc gcttcgtcga cgttcgccgt gccccgccgc
     4261 cgcgctatca gcaggatgat cgccaggccg acgaccacct ccgccgccgc gacgacgagg
     4321 acgaagaaca cgagcacctg gcccgccacc tcgccgtgca tccgcgcgaa cgtcacgaac
     4381 acgaggttga ccgcgttgag catgagctcg acgaacatga agatcacgat cgcgttgcgg
     4441 cgcacgagca ccccgacgac gccgatcgtg aacatcagcg cggcgaggac caggtagtag
     4501 ccggggtcga cgccgttcat gcaggctcct cgactcggcg gctccccgtc tcgcccatgc
     4561 ggcgggcgag gaccatgacg ccgacgatcg cgaccacgag caggatcgac gtgacctcga
     4621 acgggagcag gtagcgggag aacaggatct gtgccagggc cttcacgttg cccgggtcgc
     4681 ggttgacggc ctcgagcgcc tccggccccc cggcgccgcc tccggcgacg aagtcgaccc
     4741 cccgcagcac gacgtagagc acctgcgccg cgagcagcgc gccgagcccc agcgcgagcg
     4801 gacgctgccc gggcagcggc tccatcaagg cctcgcgctt gtcgacgccg agcagcatga
     4861 tcacgaacag gaacagcacc atgatcgccc cggcgtagac gatgatctgg acgatcgcga
     4921 cgaagaacgc tccctggagc aggaacgcga cggcgagcgc gacctgcgac cccacgagga
     4981 acagcgcgac gtgcaccgcg ttcggagcga agagcat
//
LOCUS       DASQDO010000168        12226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_690156, whole genome shotgun sequence.
ACCESSION   DASQDO010000168 DASQDO010000000
VERSION     DASQDO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690156"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2953)
                     /gene="dnaE"
                     /locus_tag="VEV43_04920"
     CDS             complement(<1..2953)
                     /gene="dnaE"
                     /locus_tag="VEV43_04920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYP22893.1"
                     /translation="MSFVHLHAHTEYSMLDGASRIGEMFEAAGNAGMPACAITDHGVM
                     YGALEFYKSGAKSGVKPILGMEGYLFLGDRREKPPQRDDTAKTCHLTLLAMDNTGYKN
                     LVKLSSRAWLEGFHYRPRADHEILAEHSEGLICLSGCLSAEIPKLIVAGQLAEARKRV
                     AYYREVFGDRFYLELQEHGIDVQKPLNDELVKIGKEMGIPWVCTNDSHYTHRDDAAGH
                     DVLLCINTGTELSDPNRFKFDSDEFYLKTPEEMAKKFERWPGACETTLEVAERCNVDI
                     DMGNLLLPRYEVPKGHSLETYLEELVLKGVRDRYDEITDEVTSRVRYELDVINKMKYP
                     GYFLVVQDFVNWAKEQGIRVGPGRGSAAGSVVSYALGITELDPLRYDLMFERFLNPER
                     IAMPDIDIDFDERRRGDVIRYVSDKYGEDHVAQIVTYGTIKGKQAIKDAARVLGFPYA
                     EGDRLTKMYPPLIMGRDSGLQSALETSPELREAYAAGGAAKEIIDTALKVENLKRSAG
                     IHAAGVVIGRDPLVEHVPLKRGDHGEVVTQFDMNGVEELGLLKMDFLGLRNLTVIELA
                     LEYIRTNKGEEVDVDSLDLTDPLVYEMLQKGDSDGVFQLESEGMRRLLCQLRPDRFEQ
                     IMALIALYRPGPMEQIPAYIECKNDPSKVRYLHSELEEITRETYGVLVYQEQIVLLLQ
                     KLAGYTPGQADLVRKAIGKKKRDIMAAEEPRFIEGCGKVGLGKSDAEKIWKLIQPFAD
                     YSFNRAHSACYAYVAYQTAWLKAHHPVEYMAALLTSVKDRKDDKPKYLHMARKMGIPV
                     LIPDVNESEVDFTPAGGSIRFGLSAVRHVGEGVVAKIIEARTRKGRFESFFDFCRKVD
                     YICLNKKTIESLIKAGAFESLGHTRRGLLDVYEDVTAEICSQRKQEEAGQFSLFGGGG
                     APDANGVTHADRAIPLDEDPKSVLLAQEKEMLGLFVSDHPLLGIEGLLARLCDCSITT
                     LENKAPGEVLTV"
     gene            complement(3100..4020)
                     /locus_tag="VEV43_04925"
     CDS             complement(3100..4020)
                     /locus_tag="VEV43_04925"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYP22894.1"
                     /translation="MKDVSFSAGPDEAGTRLDVAVAKRASVTRGLAQAAIKEGTVTVG
                     GRAVRPSHRLEEGEEVSGTVSVPTVELPQGEDIDLVLRYEDERLLVVSKPAGLVTHPA
                     RGHDTGTLVNALLGLGVPLAAATSIRPGIVHRLDKDTSGLLLVAKDDDAQAFLVDALR
                     ERRIERRYVALVRGRPPADSGTIEAPIGRHPTKRRLFAVVGDGRHSVTHYEIAQVGDR
                     LALLDLKLETGRTHQIRVHLAHLGNPVLGDRVYGGVSELSRELGLTRPFLHARSLRFP
                     HPDDGREIHVEDELPQDLAEALERAGLAAV"
     gene            complement(4047..4994)
                     /locus_tag="VEV43_04930"
     CDS             complement(4047..4994)
                     /locus_tag="VEV43_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP22895.1"
                     /translation="MNLARLCSVLGALMLFLPVSPAHAAAARDCFGKEPTIVGTAGDD
                     FLEGTSKADVIQGLEGDDTIYGYAQNDLLCGDEGNDVLEGVIGHDKLSGGSGDDRLHP
                     GAGNDLLRGGSGRDRLLSDSGDQTYDGGPGRDQVSFEIGYRPVTIDLARGTAESSRGA
                     YTLRRVEHVHGSGAADVIRGDGGRNQLVGGGGRDRIFGRGGRDHITGDGSEHGRPAGD
                     YLSGGGGDDTIAGDESNLDGGSDVIRGGDGDDVLYGDVMSGDAVGGPDRMFGGGGADR
                     LYGEDGDDELDGGEGADTGDGGPDADSCVAVENETSCER"
     gene            complement(5075..6010)
                     /locus_tag="VEV43_04935"
     CDS             complement(5075..6010)
                     /locus_tag="VEV43_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP22896.1"
                     /translation="MRVLTTAALLVALSLPAAPVAAQIPDCFGREPTIVGTEGDDTLE
                     GTRKADVIQGLGGDDSINGFAGDDLLCGDSGDDGVIGLMGDDKLSGGLGNDDLRPGRD
                     DDLLRGGDGRDRMLSDDGRDRFHGGPKSDLLTFEIRHSPVTIDLAKRSATIDGVTFPV
                     KDVERVFGTSGDDVILGSSRGEELTGALGDDRIEGRGGGDLIHGDPEYLTGGDDYLSG
                     GPGGDIIAGDGGEGGGRDTIRGGDGPDTIHGDLLIDGTGHGAADRIFGGDDHDDLYGE
                     DGNDRIDGGAGNDVADGGAGNDTCKATEENTSCER"
     gene            complement(5998..6702)
                     /locus_tag="VEV43_04940"
     CDS             complement(5998..6702)
                     /locus_tag="VEV43_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22897.1"
                     /translation="MQPLRPVSITCTLALAFAALPATPAAAAAKCFGKNPTIVGTGDD
                     DRLRGTSGRDVIQARGGDDVILGGGGKDLLCGDAGDDRVSGGSGGDRISGGLDNDLLD
                     GDRGDHLIRGVCGLDTLKPSGGTDDLDGGPGRDTLTFEGRPGPVRVDFARGSATYVGA
                     VLEVRRTENAIGSGADDVLRGSKRTERLFGEGGDDRLEGRGGNDRLEGDSGNDRADGG
                     AGDDRCDAETEQRCES"
     gene            complement(6784..7497)
                     /locus_tag="VEV43_04945"
     CDS             complement(6784..7497)
                     /locus_tag="VEV43_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP22898.1"
                     /translation="MRTRLCSVAAVLTLLVPVSPARAATEACFGKEPTIVGTDGNDEL
                     RGTYGPDVIQGLGGTDDITGLSGADLICGDEGNDFLQGGTGRDKVSGGKGEDVLSPGD
                     GADLLRGGEGGDRLMPSWGSDQTIDGGPGHDLVVLTSFPKRLRIDLERGFARGGSSRY
                     VLRRMESAHSGSGDDVILGSPGPNALDGGPGDDRIRARGGDDYVYGSHGDDHGHGGSG
                     DDWCIAETAEACEPQGEEP"
     gene            complement(7535..7993)
                     /gene="lspA"
                     /locus_tag="VEV43_04950"
     CDS             complement(7535..7993)
                     /gene="lspA"
                     /locus_tag="VEV43_04950"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYP22899.1"
                     /translation="MSGYPLLGVVGALVVAADQATKTLALETLDRPVDVLGGAVTLRI
                     TINSGGAFGVLQGVPGFFLVATIGIVVGILLWARNVEHRGWLTALGLVLGGGIGNVID
                     RVIRDHGGVVDFVDLHWWPVFNVADACIVTGVGLLLLLGTRNEGDPSAKN"
     gene            complement(8001..8837)
                     /locus_tag="VEV43_04955"
     CDS             complement(8001..8837)
                     /locus_tag="VEV43_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HYP22900.1"
                     /translation="MSPAKKPAKKSAARSKTAGARAAKTSAAKSTAKKKTAAKKTVAG
                     KKAAAKKTVAPKKTAAKKPVAKKTVAKKTAAKAPAKKTAAKKPVAKKTVAKKPVAKKT
                     VAPKTTAKKPVAKKTVAPKTTAKPAAPKKTAAAKPAARPQPSRGGAPAPRPARPAKLD
                     KKQLAAIRERLLEEKSQLEHQQNELDAEGTSGESLAEAGLDDDFADAGTATFDRERDL
                     SIRNNITDLIDQVTRAIARIDEGSYGTCERCGRPIDAARLKALPHALLCLDCKRREER
                     GR"
     gene            complement(8812..9069)
                     /locus_tag="VEV43_04960"
     CDS             complement(8812..9069)
                     /locus_tag="VEV43_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HYP22901.1"
                     /translation="MWAGVHGTGLRVKVKAPPVEGRANRAVEELFAGLLGLPRSAVRV
                     VTGELSRHKTIHVSGMSPEDVTIRLDGVLSSRAHEPGQEAR"
     gene            9243..>12226
                     /gene="ileS"
                     /locus_tag="VEV43_04965"
     CDS             9243..>12226
                     /gene="ileS"
                     /locus_tag="VEV43_04965"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HYP22902.1"
                     /translation="MPERSYRSVDPHVSWPDMEAAVAARWRELDVFHRTLEVREGTPE
                     WVFYDGPPTANNKPGIHHVEPRAFKDVYCRFQVMRGHYVARKAGWDCHGLPVEIEVEK
                     SLGITQKREIEEKVGIEEFTRLCRESVQRYVDDHARLTDRIGYWVDQDDAYWTMSRDY
                     VESVWWILKQIWDKGLLEEDFKVVPYCPRCETSLSSHEQHQTDTYRDVVDPSVFVRFP
                     LAADPSTALLVWTTTPWTLLSNMAAAVGPDITYVRVADPGKEGSFLLLARERVEARLG
                     EGAEVLEEIHGGDLVGRIYSPPFGFVEPGETGHRVRPGDFVTTEDGTGIVHLAPYGEA
                     DMEVAKRDDLPIVGMIDPSGKVVPRGEPFAGLWVKDADPKIIEDLESRGLLFKAEDYE
                     HAYPHCWRCKTPLLYYPRKDWYVRTSQIRGDLQASNEDTNWQPPTIKHGRFGDWLANN
                     VDWSLSRDRYWGTPLPVWRCEANHATCVGSIAELAEHAGRDLSDLDPHRPYVDEVTIT
                     CPECHAEARRVKSVIDAWFDSGSMPFAQWHYPFENEDVFEARFPADFICEAIDQTRGW
                     FYSLLAVATLVRGGNSYRNVVVLGHIVDAQGRKMSKSLGNVIDPWLVLDAQGADALRW
                     YLLTSGSPWSARRVSVELIEETLRKYLLTLWNTYSFWVTYASLEGFDPSEHQPVTDRP
                     RIDRWILAELDDTVREVTESLETFDCTRGGRRLDRFVDDLSNWYVRRSRRRFWRSASD
                     EDTKTAFLTLHECLVTVAKLAAPYTPFVADEIYTNLTGEDSVHLSDWPEPDGFRADDD
                     LRRRMQLVRRLVTAGRSARTEAKVKVRQPLAKAIVVLPAAEAGDLAELEGLLAEELNV
                     KEIEVARGLAELVTYTVKPNFKALGPRFGKQVKDVAAALSRVDAYELVGALERDGSAT
                     VEVGGEHVALTRDELDVRVAGREGFSLAQDGPYGVALDLDVTPELAAEGAAREVVRAV
                     QDLRKSSGLAVEDRIELWIAGGT"
BASE COUNT         1685 a         4302 c         4208 g         2031 t
ORIGIN      
        1 cgacggtcag cacctcgccc ggagccttgt tctcgagcgt cgtgatcgag cagtcacaga
       61 gacgcgccag caggccctcg atcccgagca acgggtggtc ggagacgaag aggccgagca
      121 tctccttctc ctgcgcgagc agcacgctct tggggtcctc gtcgagaggg atggcgcggt
      181 ccgcgtgcgt cacgccgttg gcatccggcg ccccgccccc gccgaacaac gagaactgcc
      241 cggcctcctc ctgcttgcgc tgggaacaga tctcggcggt gacgtcctcg tagacgtcga
      301 gcaggccgcg gcgggtgtgc cccagcgact cgaacgcacc ggccttgatc aaggactcga
      361 tcgtcttctt gttcaggcag atgtagtcga ccttgcggca gaagtcgaag aacgactcga
      421 agcgaccttt gcgcgtgcgc gcctcgatga tcttggcgac gacgccctca cctacgtgac
      481 ggaccgcgga gagcccgaag cggatcgaac ctccggccgg cgtgaagtcg acctcggact
      541 cgttcacgtc cgggatgagg acggggatcc ccatcttgcg cgccatgtgc aggtacttgg
      601 gtttgtcgtc cttgcggtcc ttcacggacg tcagcagcgc ggccatgtac tcgaccgggt
      661 ggtgggcctt cagccacgcg gtctggtacg cgacgtacgc gtagcaggcg gagtgcgcgc
      721 ggttgaacga gtagtcggcg aacggctgga tgagcttcca gatcttctcc gcgtcggact
      781 tgccgagacc gaccttgccg cagccctcga tgaagcgcgg ctcctcggcg gccatgatgt
      841 cccgcttctt cttgccgatg gccttccgga ccaggtcggc ctggccgggc gtgtagccgg
      901 cgagcttctg cagcaggagg acgatctgct cctggtagac gagcacgcca taggtctcgc
      961 gcgtgatctc ttcgagctcg gagtggagat agcggacctt cgacgggtcg ttcttgcact
     1021 cgatgtacgc cgggatctgc tccatgggcc cggggcggta gagcgcgatg agggccatga
     1081 tctgctcgaa gcggtcggga cgcagctggc acagcagcct gcgcatccct tccgactcga
     1141 gctggaagac gccgtcggag tcgcccttct gcaacatctc gtagacgagc gggtcggtca
     1201 ggtcgaggct gtcgacgtcg acctcttcgc ccttgttcgt gcggatgtac tcgagcgcga
     1261 gctcgatcac ggtgaggttc cggaggccga ggaagtccat cttgaggagc ccgagctcct
     1321 cgaccccgtt catgtcgaac tgcgtgacga cctcgccgtg gtcgccgcgt ttgagcggca
     1381 cgtgctcgac gagagggtcg cggccgatca cgacgcccgc cgcgtggatg ccggcggagc
     1441 gcttgaggtt ctcgaccttc agcgcggtgt cgatgatctc cttcgcggcg cccccggccg
     1501 cgtacgcctc gcgcagctcg ggcgacgtct cgagcgcgct ctgcagcccc gagtcgcggc
     1561 ccatgatcag cggcgggtac atcttcgtca ggcggtcgcc ttccgcgtag gggaagccga
     1621 gcacgcgggc cgcgtccttg atcgcctgct ttcccttgat cgtgccgtac gtgacgatct
     1681 gcgcgacgtg gtcctcgccg tacttgtcgc tcacgtagcg gatgacgtcg ccgcgccggc
     1741 gctcgtcgaa gtcgatgtcg atgtcgggca tcgcgatgcg ttccgggttc aggaaccgct
     1801 cgaacatgag gtcgtagcgc agcgggtcga gctcggtgat gccgagcgcg tagctgacga
     1861 ccgatcccgc cgcgctcccg cggcccgggc ccaccctgat cccctgctcc ttcgcccagt
     1921 tcacgaagtc ctggaccacg aggaagtagc ccgggtactt catcttgttg atgacgtcga
     1981 gctcgtagcg gaccctgctc gtgacctcgt ccgtgatctc gtcgtagcgg tcgcgcacgc
     2041 ccttcagcac gagctcttcg aggtaggtct cgagcgagtg ccccttcggg acctcgtagc
     2101 gagggagcag gaggttgccc atgtcgatgt cgacgttgca gcgctcggcc acctcgagcg
     2161 tcgtctcgca cgcgcccggc caccgctcga acttcttcgc catctcctcc ggcgtcttca
     2221 ggtagaactc gtcggagtcg aacttgaagc ggttcgggtc ggacagctcg gtccccgtgt
     2281 tgatgcacag cagcacgtcg tggccggcgg cgtcgtccct gtgggtgtag tgcgagtcgt
     2341 tggtgcagac ccacgggatc cccatctcct tgccgatctt cacgagctcg tcgttcagcg
     2401 gcttctgcac gtcgatgccg tgctcttgga gctcgaggta gaagcggtcg ccgaagacct
     2461 cgcggtagta ggcgacgcgc ttcctcgcct cggcgagctg tcccgcgacg atcagcttgg
     2521 ggatctcggc cgacaggcag ccggagaggc agatgagccc ttcggagtgc tcggcgagga
     2581 tctcgtggtc ggcgcggggc cggtagtgga agccttcgag ccacgcccgc gacgagagct
     2641 tgacgaggtt cttgtagccc gtgttgtcca tcgcgagcag cgtgagatgg cacgtcttcg
     2701 cggtgtcgtc gcgctggggg ggcttctcgc ggcggtcacc gaggaagagg tagccctcca
     2761 tgccgaggat gggcttgacg ccggacttgg cgccgctctt gtagaactcg agggcgccgt
     2821 acatgacgcc atggtcggtg atcgcgcagg ccggcatccc tgcgttgccg gcggcttcga
     2881 acatctcgcc gatacggctg gccccgtcga gcatggagta ctcggtgtgg gcatggagat
     2941 gcacgaagct cactgctaca ggtctcccct cgagatccag gcggcggaat cacccgagtg
     3001 taattcccgg cgcggcacgg gccgcggact tctgcccagc caaccggatc gggcctgggg
     3061 gacgtaagta cgacttcctg tgagccggct gtggagaagc tagacggcgg ccaatccggc
     3121 ccgctccagg gcctccgcca ggtcctgcgg cagctcgtct tccacgtgga tctcccggcc
     3181 gtcgtcgggg tgcgggaagc ggagcgagcg ggcgtggagg aacggcctcg tcaggcccag
     3241 ctcccgggac agctccgaga cgccgccgta gacccggtcg cccaggaccg ggttgccgag
     3301 gtgcgctagg tggaccctga tctggtgcgt gcggccggtc tcgagcttga ggtcgagcag
     3361 cgccaaccgg tcccccacct gagctatttc gtagtgcgtg accgagtggc gtccatcgcc
     3421 gacgacggcg aacagccggc gcttcgtggg atggcggccg atcggggctt cgatcgtgcc
     3481 cgagtccgcc ggcgggcggc cccgcacgag cgctacgtag cgccgctcga tccgccgctc
     3541 gcggagcgcg tcgacgagga aggcctgggc gtcgtcgtcc ttggccacga gcagcagccc
     3601 cgacgtgtcc ttgtcgagcc ggtggacgat cccgggccgg atcgaggtgg ccgcggcgag
     3661 cgggacgccg aggccgagca gcgcgttcac cagcgtgccg gtgtcgtgac cgcgcgccgg
     3721 gtgggtgacg aggcccgccg gcttcgagac gacgagcagc cgctcgtcct cgtaccgcag
     3781 gacgagatcg atgtcttcgc cctgcgggag ctcgaccgtc ggcaccgaca cggtgccgga
     3841 cacttcctcg ccctcttcga gccggtggct cggcctgacg gcgcgacccc cgaccgtcac
     3901 ggtcccctct ttgatcgcgg cctgggcgag gccccgcgtg accgaagcgc gcttggcgac
     3961 cgcgacgtcg agccgggtcc ctgcttcgtc cggccccgcg gagaaagaaa cgtccttcac
     4021 ggccgggacg ctaccggctc accggctcac cgctcgcagc tcgtctcgtt ctcgacggcc
     4081 acgcacgagt ccgcgtccgg gccgccgtcg ccggtgtcgg caccctcgcc gccgtcgagc
     4141 tcgtcgtccc cgtcctcccc gtagagcctg tcggcgccgc cgcctccgaa catgcggtcg
     4201 gggccgccga cggcgtcgcc ggacatgacg tcgccgtaca ggacgtcgtc gccgtcgcct
     4261 ccgcggatca cgtcggatcc accgtcgagg ttgctctcgt cgccggcgat cgtgtcgtcg
     4321 ccccctccgc ccgacaggta gtcgccggcg ggcctcccgt gctcggagcc gtcacctgtg
     4381 atgtggtccc tgccgccccg gccgaagatc cggtcgcggc ccccgccccc gacgagctgg
     4441 ttgcgacccc cgtcaccccg gatgacgtcg gcagcgcccg acccgtggac gtgctcgacg
     4501 cgccgcaagg tgtacgcgcc gcgcgagctc tccgcggtgc cgcgcgccag gtcgatcgtc
     4561 accgggcggt acccgatctc gaacgagacc tggtccctgc cggggcctcc gtcgtatgtc
     4621 tggtcgccgc tgtcgctcag caggcgatcg cgaccgctac ccccgcgcag gaggtcgttc
     4681 cccgcaccgg ggtgcagcct gtcgtcaccg ctccccccgc tcagcttgtc gtggccgatc
     4741 acgccctcga ggacgtcgtt gccctcgtcg ccgcagagca agtcgttctg cgcgtagccg
     4801 taaatggtgt cgtcgccctc gagaccctgg atgacgtcgg ccttcgacgt cccctcgagg
     4861 aagtcgtcgc cggcggtccc gacgatcgtc ggctccttgc cgaagcagtc gcgggcggcg
     4921 gccgcgtgcg caggcgacac gggaaggaag agcatcagcg ctccgagcac ggagcagagg
     4981 cgggcgaggt tcatcttggg actcctgacg aagtgtgggg ttcctcccac gatgaaccgg
     5041 cgcggcgccg cgctccactc aagtctttac agagtcaccg ctcgcacgag gtgttctcct
     5101 ccgtcgcctt gcaggtgtcg ttgccggcgc ctccgtccgc gacgtcgttg cccgcgccgc
     5161 cgtcgatccg gtcgttgccg tcctccccgt acagatcgtc gtggtcgtcc ccaccgaaga
     5221 tgcggtcggc cgcgccgtgg ccggtcccgt cgatcagcag gtcgccgtgg atcgtgtccg
     5281 gtccgtctcc gccgcggatc gtgtcgcggc ccccgccctc gccgccgtca cccgcgatga
     5341 tgtcaccgcc gggaccgccg gagaggtagt cgtcgcctcc cgtgaggtac tcggggtccc
     5401 cgtggatgag atcccctccg cctctgccct cgatgcggtc gtctcccagg gccccggtca
     5461 gctcctcgcc ccgagagctc ccgaggatga cgtcgtctcc cgacgtgccg aagacgcgct
     5521 cgacgtcctt caccgggaac gtgacgccgt cgatcgtcgc ggagcgcttg gcgaggtcga
     5581 tcgtgaccgg gctgtgccgg atctcgaacg tcaggaggtc gctcttcggg ccgccgtgga
     5641 agcggtcgcg cccgtcgtcg ctcagcatcc ggtcgcggcc gtcacccccg cgcagcaagt
     5701 cgtcgtcgcg gccgggcctc aagtcgtcgt tgcccagacc gccgctcagc ttgtcgtcac
     5761 ccatcagccc gatcaccccg tcgtcgccgg agtcgccgca caggaggtcg tcgccggcga
     5821 acccgttgat cgagtcgtcg ccaccgaggc cctggattac gtcggccttt cgtgtgccct
     5881 ccaaggtgtc gtcgccttcg gtcccgacga tcgtcggctc gcggccgaag cagtcgggga
     5941 tctgcgccgc gacgggggcc gcgggaagcg agagcgcgac cagcagcgcg gccgtggtca
     6001 ggactcgcat cgctgctcgg tctcggcgtc gcagcgatcg tctcccgcgc ctccgtccgc
     6061 cctgtcgttg ccggagtcgc cctcgagacg gtcgttcccg ccccggccct cgaggcggtc
     6121 gtcgccaccc tcgccgaaga gcctttcggt gcgcttgctg ccccggagca cgtcgtccgc
     6181 ccccgagccg atcgcgttct ccgtccggcg gacctccagg acggcgccca cgtacgtcgc
     6241 actgccccgg gcaaagtcga cacggaccgg gccgggacgg ccttcgaacg tcagggtgtc
     6301 gcggcccggg cctccgtcga ggtcgtcggt gccgccgctg ggcttcagcg tgtcgagccc
     6361 gcagacccca cggattaggt ggtcaccgcg gtcgccgtcg agcaagtcgt tgtcgagacc
     6421 cccgctgatc ctgtcgccgc cgctgccccc cgagacccgg tcgtcacccg cgtcaccgca
     6481 caggaggtct ttgcccccgc ctccgaggat gacgtcgtca ccgccgcggg cctggatgac
     6541 gtcgcggccc gaggtcccgc gcagcctgtc gtcgtcaccg gtgccgacga tcgtcgggtt
     6601 cttgccgaag cacttggccg cggcggccgc cggcgtggcc gggagcgcgg cgaacgcgag
     6661 cgcgagcgtg cacgtgatcg aaacggggcg aagcggctgc atgggatccc tcctctggtc
     6721 ggggccttgc accgcgatcg ttcccggggc ggcccgctct atgcgtaagt agccggtccc
     6781 gtcctagggc tcctcgccct gcggctcgca cgcctcggcc gtctcggcga tgcaccagtc
     6841 gtcgccggaa ccgccgtgcc cgtggtcgtc gccgtgggac ccgtagacgt agtcgtcccc
     6901 gccgcgggcc ctgatgcggt cgtcgccggg gccgccgtcg agcgcgttcg ggcccggcga
     6961 gccgaggatc acgtcgtcgc cggaaccgct gtgcgcggac tccatccgcc ggaggacata
     7021 ccggctggac cctccgcgag cgaatccacg ctctagatcg atgcgcagtc gcttggggaa
     7081 gctggtcaag acgacgaggt cgtgaccggg gccgccgtcg atcgtctggt ccgaccccca
     7141 gctcggcatc aagcggtcgc ccccctcgcc cccgcgcagc aggtcggcgc cgtccccggg
     7201 cgacaggacg tcctcgccct tgccgccgct caccttgtcg cgccccgtgc cgccctggag
     7261 gaagtcgttg ccctcgtcgc cacagatgag gtcggcgccc gacaggcccg tgatgtcgtc
     7321 ggtgcctccg aggccctgga tgacgtcggg accgtacgtg ccgcggagct cgtcgttccc
     7381 gtcggttccg acgatcgtcg gctctttccc gaagcacgcc tctgtggcgg cccgtgccgg
     7441 cgacaccggc acgaggaggg tcaggacggc ggcgacggag cagaggcgtg tgcgcatgac
     7501 ggccacgctg gcaccgagct cgtgctcgta ctactcagtt ctttgcagac ggatcgcctt
     7561 cgttgcgcgt cccgaggagg agcagcaggc cgacgccggt cacgatgcac gcgtcggcga
     7621 cgttgaagac cggccaccag tggaggtcga cgaagtcgac gaccccgccg tggtcacgga
     7681 tcacccggtc gatcacgttc ccgatgcccc cgccgagcac gaggccgagc gcggtcagcc
     7741 acccgcggtg ctcgacgttg cgcgcccaca agaggatccc gacgacgatc ccgatcgtgg
     7801 cgacgaggaa gaagccgggc acgccctgca ggacgccgaa cgcgccgccg gagttgatcg
     7861 tgatcctcag cgtcaccgcg cccccgagca cgtccacggg ccggtcgagg gtctcgagcg
     7921 cgagcgtctt cgtcgcctgg tccgcggcta cgacgagcgc gccgacgacg ccgaggaggg
     7981 ggtagccgga caagcggaag ctagcggccg cgttcttcgc ggcgcttgca gtcgaggcag
     8041 aggagcgcgt gcggcagggc cttcaggcgg gccgcgtcga tcgggcgccc gcaccgttcg
     8101 caggtcccgt aggacccctc gtcgatgcgc gctatcgcgc gcgtgacctg gtcgatgagg
     8161 tcggtgatgt tgttgcggat cgagaggtcg cgctcgcggt cgaacgtggc ggtcccggca
     8221 tcggcgaagt cgtcgtcgag cccggcttcc gcgagcgact cgcccgaggt cccttcggcg
     8281 tcgagctcgt tctgctggtg ttcgagctga gacttctcct cgagcagccg ttcgcggatc
     8341 gccgcgagct gcttcttgtc gagcttcgcc ggacgggcgg gccggggggc cggggccccg
     8401 ccccgggagg gttgcggccg cgccgcaggc ttggccgcgg cggtcttctt cggagcggcg
     8461 ggcttcgcgg tcgtcttcgg cgcgaccgtc ttcttcgcga ccggcttctt ggcggtggtc
     8521 ttcggggcga ccgtcttctt ggctacgggc ttcttcgcaa ccgtcttctt ggctacgggc
     8581 ttcttcgcgg cggtcttctt ggccggggcc ttcgctgcgg tcttcttggc gaccgtcttc
     8641 ttcgccaccg gcttcttggc cgccgtcttc ttcggagcga cggtcttctt cgccgcggcc
     8701 ttcttccccg ccacggtctt cttggccgcg gtcttcttct tggccgtgga cttggcggcg
     8761 gaggtcttcg cggcgcgcgc tcccgcggtc ttcgagcgag ccgcgctctt cttagcgggc
     8821 ttcttggccg ggctcatggg cgcgggacga tagcacaccg tcaaggcgga tagtcacgtc
     8881 ttccggcgac atcccggaga cgtggatcgt cttgtggcgg ctcaactcgc cggtcaccac
     8941 gcggaccgcg ctgcggggca acccgaggag gccggcgaac agctcctcga ccgcgcgatt
     9001 ggcccggccc tcgaccggcg gtgccttcac cttgaccctc agcccggtgc cgtggacgcc
     9061 cgcccacaaa ccgacgtttg aaccatccca atgacggcag gtttcggtac cacctcaccg
     9121 ttcaaacgtc ggtttggcat ccttcgcagc ccttggacgg acgaagacct ctatccccgc
     9181 gccgccgcgg acgggccgta tccaggcggg aagcgacatc gccgcaagct aacctgtgcg
     9241 ccatgccgga gcgcagctac cgatccgtcg acccgcacgt ctcgtggccc gacatggagg
     9301 ccgcggtcgc cgcacgctgg cgggagctcg acgtcttcca ccgcacgctc gaggtccgcg
     9361 aagggacccc ggagtgggtc ttctacgacg gtcccccgac cgccaacaac aagcccggca
     9421 tccaccacgt cgagccccgt gcgttcaagg acgtgtactg ccgcttccag gtcatgcgcg
     9481 ggcactacgt cgcccgcaag gccgggtggg actgccacgg cctccccgtc gagatcgagg
     9541 tcgagaagag cctcggcatc acccagaagc gcgagatcga ggagaaggtc gggatcgagg
     9601 agttcacgcg gctgtgccgc gagtcggtcc agcgctacgt cgacgaccac gctcgcctca
     9661 cggatcgcat cggctactgg gtcgaccagg acgacgcgta ctggacgatg tcacgggact
     9721 acgtcgagag cgtgtggtgg atcctcaagc agatctggga caagggcctc ctcgaagagg
     9781 acttcaaggt cgtcccctac tgcccgcgtt gcgagacgtc gctgtcgtcg cacgagcagc
     9841 accagaccga cacctacaga gacgtcgtcg acccgtcggt gttcgtgcgg ttcccgctcg
     9901 cggccgaccc gtcgacggcg ctgctcgtgt ggacgacgac gccgtggacg ctgctgtcga
     9961 acatggccgc cgcggtgggc ccggacatca cgtacgtacg ggtcgccgac cccgggaaag
    10021 aggggtcctt cttgctcctc gcccgcgagc gcgtcgaggc ccgtctcggc gagggggccg
    10081 aggtgctcga ggagatccac ggcggggacc tcgtcggacg catctactcg ccgccgttcg
    10141 ggttcgtcga gccgggcgag accggacacc gcgtccggcc cggggacttc gtcacgaccg
    10201 aggacgggac ggggatcgtc cacctcgctc cctacggcga ggccgacatg gaggtggcca
    10261 agcgcgacga cctcccgatc gtcgggatga tcgacccctc gggcaaggtc gtgccgcgcg
    10321 gcgagccgtt cgccggcctg tgggtgaagg acgccgaccc gaagatcatc gaggacctcg
    10381 agtcgcgggg cttgctgttc aaggccgagg actacgagca cgcgtatccg cactgctggc
    10441 gctgcaagac gccgctcctc tactacccgc gcaaggactg gtacgtgcgg acgtcgcaga
    10501 tccgcggcga cctccaggcg agcaacgagg acacgaactg gcaaccgccc acgatcaagc
    10561 acggccgctt cggcgactgg ctcgcgaaca acgtcgactg gtcgctgtcg cgcgaccgct
    10621 actggggcac gcctctgccg gtgtggcgct gcgaggcgaa ccacgcgacg tgcgtgggct
    10681 cgatcgcgga gctcgccgag cacgccggac gcgacctctc cgacctcgac ccgcaccgtc
    10741 cctacgtaga cgaggtcacg atcacttgcc ccgagtgcca tgcggaggcg cgccgcgtga
    10801 agagtgtgat cgacgcgtgg ttcgactccg gctcgatgcc gttcgcccag tggcactacc
    10861 cgttcgagaa cgaagacgtg ttcgaggcgc gcttccccgc ggacttcatc tgcgaggcga
    10921 tcgaccagac gcgcgggtgg ttctactcgc tgctcgcggt cgcgacgctc gtgcgcggcg
    10981 ggaactcgta ccgcaacgtc gtcgtcctcg gccacatcgt cgacgcgcag gggcgcaaga
    11041 tgtcgaagtc tctcggcaac gtcatcgacc cgtggctggt gctcgacgcg cagggggccg
    11101 acgcgctgcg ctggtacctg ctgacgtcgg gctcgccgtg gtcggcacgc cgcgtctcgg
    11161 tcgagctgat cgaggagacg ctgcgcaagt acctgctgac gctgtggaac acgtactcgt
    11221 tctgggtgac gtacgcgtcc ctggagggct tcgacccgtc cgagcaccag ccggtgaccg
    11281 accggccccg catcgaccgc tggatcctcg ccgagctcga cgacaccgtt cgcgaggtga
    11341 ccgagtcgct cgagactttc gactgcacgc gcgggggccg gcgcctcgac cggttcgtcg
    11401 acgacctctc gaactggtac gtgcgccgga gccggcgtcg cttctggcgg tcggcgtcgg
    11461 acgaggacac gaagaccgcg ttcctgacgc tgcacgagtg cctcgtcacc gtcgcgaagc
    11521 tcgcggcccc ctacacgccg ttcgtcgccg acgagatcta cacgaacctg accggtgagg
    11581 actcggtcca cctctccgac tggcccgagc ccgacggctt ccgcgccgac gacgacctcc
    11641 gccggaggat gcagctcgtg cgccggctcg tgaccgcggg gaggtcagcg cggaccgagg
    11701 cgaaggtcaa ggtgcgccag ccgctcgcga aggcgatcgt cgtcctgccc gcggccgagg
    11761 ccggcgacct cgccgagctc gaggggctgc tcgccgagga gctcaacgtg aaggagatcg
    11821 aggtcgcccg cgggctcgcc gagctcgtca cctacacggt caagccgaac ttcaaggcgc
    11881 tcggcccccg cttcggcaag caggtgaagg acgtcgcggc cgctctctcc cgcgtcgacg
    11941 cgtacgagct cgtcggcgcg ctcgagaggg acggctccgc gacggtcgag gtgggcggcg
    12001 agcacgtggc gctgacgcgc gacgagctcg acgtcagggt cgccgggcgc gaggggttct
    12061 cgctcgcgca ggacgggccc tacggggtcg cgctggacct cgacgtgacg ccggagctcg
    12121 cggccgaggg ggccgcgcgt gaggtcgtgc gcgcggtgca ggacctgcgg aagtcgagcg
    12181 ggctcgcggt cgaggaccgc atcgagctgt ggatcgccgg cggcac
//
LOCUS       DASQDO010000169         4037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_78676, whole genome shotgun sequence.
ACCESSION   DASQDO010000169 DASQDO010000000
VERSION     DASQDO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78676"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VEV43_04970"
     CDS             complement(<1..902)
                     /locus_tag="VEV43_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP22903.1"
                     /translation="MHPQPELVAEADPVTAAASVLLCDDAADIRALLDAAIADHPDME
                     VVGEAATGLEAIEQARLLQPDVVVLDLSMPVMDGFEALPRILSVAPQAKVVAFSGRTA
                     AEAEASAVAAGATSYIPKGARVEEIVAALRDAAGLPPHAAVRSVTRDQELRLFSFSLD
                     LLCIADQDGYFRHLSPSWERTLGWSVEELKTRPFLEFVHPHDVASTQSEMARLLEGQD
                     TVGFENRYASRNGPYRWLSWRATPTPEGLIYASARDVTEEKRAAASRDTLATIVEWSF
                     DALVGVVLEVTSTIGNAAAETLYGR"
     gene            complement(1036..1692)
                     /locus_tag="VEV43_04975"
     CDS             complement(1036..1692)
                     /locus_tag="VEV43_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP22904.1"
                     /translation="MSGHDAAPVRVLLVDDHDVVLHGLAEFFDHEPGVAVAGRARTGE
                     DALALLASTAADVALIDLRLPSMDGIALCREIRQAFPATRCLILTSFGDEDSVIEAMA
                     CGASGFVLKESPLEEVARAVKKVAGGESPIDPSLTAQVFARLRSGPKAHKLELLTAQE
                     RRILELVSEGLSNKQIAERLFLADQTVKNYVSSILSKLEVQGRTQAAVFLLQRGAPPP
                     "
     gene            complement(1679..3013)
                     /locus_tag="VEV43_04980"
     CDS             complement(1679..3013)
                     /locus_tag="VEV43_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYP22905.1"
                     /translation="MAARGNTTTAPSLKVVGTYLVVSVSWIALSDQLVTRLASGRDQL
                     TFLQTVKGWAFVGLSALGLYVLVSRALRSAADAASDILQREAQIDLVLGQIPANIWTT
                     DERLRITSLRGRGVLGPLRGTRTQPLEASQVVGTTVYERFGTEDPAAPPVGAHLRALE
                     GHETEYTVDWEGRTSGSVVTPLRDPEGNSIGCLGFSIDVTDQVEIERERLRSLSRLER
                     ANTRAGRLLQHLVRAEGEERKRIAVGIHDDSIQAMTSAGMALDLLVARLPASSDADLA
                     RRARDLVRDSMRRLRSLVFELRPMELDRSGLAPSILLLLQQTAAESRVRFDFSDETSA
                     PLDPDVAFLVYRIVQEALANVRKHARATLVTVTLSEADGGILTVVKDDGVGFDSSGDL
                     PPDHFGLRDMSERAEIAEGWCRIESTPSEGTVVEAWVPRAVAVTEEVGRVRS"
     gene            complement(3117..>4037)
                     /locus_tag="VEV43_04985"
     CDS             complement(3117..>4037)
                     /locus_tag="VEV43_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYP22906.1"
                     /translation="DFDMPGGSGLDLLQHVQARCPDTGTIMLTGKGSREVGEAAVRVG
                     VYGYLRKPLDVEEMLVCVFNALTRRQLTIDNRNHHLRLEELVAARTSELWQSTLQLER
                     SHQALRESRRETIHRLVLASELHDDETGAHVRRMSLYCRLLVAHATGDDERAEDVELA
                     SMMHDVGKIGIPDRILRKNGKLTADELEEMKRHAVIGHNLLAASESPLLDLGATIALT
                     HHEWFDGSGYPHGLAGAAIPLEGRVAAVADVFDALTTHRVYRKAFDLQDAVDIMRAER
                     GTHFDPDLLDLFLDLLPDVLRIKGAAAEPA"
BASE COUNT          604 a         1386 c         1430 g          617 t
ORIGIN      
        1 cgcccgtaga gggtctctgc cgcggcgttc ccgatggtgc tcgtcacttc caggacgacg
       61 ccgacgagcg cgtcgaacga ccactccacg atcgtcgcga gcgtgtcgcg gctggcggcc
      121 gcgcgcttct cctcggtcac gtcacgggcg gaggcgtaga tgaggccttc cggcgtcggc
      181 gtcgcgcgcc acgagagcca gcgatagggc ccgttgcggg acgcgtagcg gttctcgaag
      241 cccacggtgt cctggccctc gagcagccgc gccatctcgg attgggtcga cgcgacgtcg
      301 tgggggtgga cgaactccag gaacggtcgc gtcttcagct cctcgacgct ccaccccagc
      361 gttcgctccc agctcgggct gaggtgccgg aagtagccgt cctggtccgc gatgcacagg
      421 aggtccagcg agaacgagaa gagtcgcagc tcctggtcgc gggtgaccga gcggacggcg
      481 gcgtgcggcg ggaggccggc cgcgtcccgg agcgccgcga cgatctcctc gacgcgggcc
      541 cccttcggga tgtacgacgt cgccccggct gcgaccgccg acgcctcggc ctctgcggcg
      601 gtgcggccgg agaacgcgac taccttcgct tggggggcca ccgacaggat gcgggggagt
      661 gcttcgaaac cgtccatgac cggcatcgac aggtcgagca ccaccacgtc cggctgcagc
      721 agcctcgcct gctctatggc ctcgagcccg gtggcggcct cgccgacgac ctccatgtcg
      781 ggatggtccg cgatggcggc atcgaggagg gcgcggatgt cggcggcatc gtcgcagaga
      841 aggacggaag ccgcggcggt cactgggtcc gcctcggcga cgagctcggg ttggggatgc
      901 acttgcgctc ctttgatcgg gactgcggtc tacggtgacg gaaggagcgg cgcggtgaca
      961 gagcgtgaag tcccgaccgc ggaggacgac tcgtaccgag ttcgcgcggt accgaggtac
     1021 tgcgcgagac cccgatcagg ggggtggggc cccccgctgg agcaggaaca ccgcggcctg
     1081 ggtccggccc tgcacctcga gcttcgacag gatgctcgag acgtagttct tcaccgtctg
     1141 gtcggccagg aacaggcgct ccgcgatctg cttgttgctg agcccctccg agacgagctc
     1201 gaggatcctg cgctcctggg cggtgagcag ctcgagcttg tgggccttcg ggccggaccg
     1261 cagccgtgcg aacacctgcg cggtcaggga ggggtcgatc ggggactcgc cgccggccac
     1321 cttcttcacg gcgcgcgcca cctcttcgag gggtgactcc ttcaacacga acccggacgc
     1381 gccgcaagcc atcgcttcga tgaccgagtc ctcgtcgccg aacgacgtga ggatgaggca
     1441 gcgggtcgcc gggaaggcct ggcggatctc gcggcacagc gctatcccgt ccatcgaggg
     1501 gagccggagg tcgatcaacg cgacgtctgc ggccgtcgac gccaggaggg ccagcgcgtc
     1561 ttcgcccgtc cgggcgcggc cggcgacggc gacgcccggc tcgtggtcga agaactctgc
     1621 gaggccgtgc agcacgacgt cgtggtcgtc gaccagcagg acgcggaccg gagcggcgtc
     1681 atgaccggac acggcccacc tcctcggtga cggcgaccgc gcgcgggacc catgcctcga
     1741 ccacggtccc ctccgaaggg gtgctctcga tgcggcacca cccctccgcg atctcggcgc
     1801 gctccgacat gtcgcgcagg ccgaagtggt cgggaggcag gtcgccggag gagtcgaaac
     1861 cgacgccgtc gtccttgacg accgtcagga tccccccgtc cgcctccgac agcgtgaccg
     1921 tcacgagcgt cgcgcgagcg tgtttgcgga cgttcgccag ggcctcctgc acgatccggt
     1981 agacgaggaa cgcgacgtcg gggtcgagcg gcgcggaggt ctcgtccgag aagtcgaacc
     2041 gcactcgtga ctccgccgcg gtctgctgca gcaggaggag gatcgagggg gccaggccgg
     2101 agcggtcgag ctccatcggc cggagctcga acaccaggct ccgcaggcgc cgcatcgagt
     2161 cgcgcaccag gtcccgggcg cgccgggcga ggtcggcgtc cgagctggcc ggaaggcgtg
     2221 ctaccaacag gtcgagcgcc atgcccgccg acgtcatggc ctggatggag tcgtcgtgga
     2281 tgccgaccgc gatgcgcttc cgttcctcgc cttcggcccg tacgagatgc tgcagcagac
     2341 ggcctgcacg ggtgttcgcg cgttcgaggc gggagagcga gcgaagccgc tcgcgctcta
     2401 tctcgacctg atcggtcacg tcgatcgaga agccgaggca gccgatcgag ttcccctcgg
     2461 ggtcgcgcag aggcgtgacc accgagccgc tggtccggcc ctcccagtcg acggtgtact
     2521 cggtctcgtg cccttcgaga gcgcggaggt gagcgccgac cggcggcgcc gccgggtctt
     2581 ccgtaccgaa ccgctcgtac acggtcgtgc cgacgacttg actcgcctcc agcggttgcg
     2641 tcctcgtccc tctcaggggc ccgaggacac cgcggcctcg gagggacgtg atccgtagcc
     2701 gctcgtccgt cgtccagatg ttcgcgggga tctgtcccag gacgaggtcg atctgcgcct
     2761 cacgctgcag gatgtcggac gccgcgtcgg ctgccgaccg cagcgctcgc gacacgagga
     2821 cgtagaggcc gagcgcgctc aggccgacga acgcccagcc cttgaccgtc tgcaggaacg
     2881 tcagctggtc ccgccccgac gcgagccgtg tgacgagctg gtccgacaat gcgatccagc
     2941 tcaccgagac caccaggtac gtgccgacga ccttgagaga aggcgccgtc gtcgtgttgc
     3001 cgcgtgccgc caaagccagc ctccttcgcc agtaggcttg ctcgaaccgc cgcaggggtc
     3061 acttgaggac cgagtgacgt gacgataata cctcggttaa gccaggatat gtcaggtcac
     3121 gcgggctccg ctgcggctcc tttgatgcgg agcacgtccg gcagcagatc gaggaagagg
     3181 tcgagcaggt cggggtcgaa gtgcgtcccc cgctcggccc gcattatgtc gaccgcgtcc
     3241 tggaggtcga aagccttccg gtacacccgg tgagtggtca gggcgtcgaa cacgtcggcc
     3301 accgccgcga cgcggccctc gagcgggatc gcagccccgg cgagaccgtg tgggtatccc
     3361 gagccgtcga accactcgtg gtgggtcagc gcaatcgtcg cccccaggtc gaggagcggg
     3421 gactccgagg ccgcgaggag gttgtgcccg atgacggcgt ggcgcttcat ctcctcgagc
     3481 tcgtcggccg tcagcttccc gttcttccgc aggatccggt ccgggatgcc gatcttgccg
     3541 acgtcgtgca tcatgctcgc cagctcgacg tcctcggcac gctcgtcgtc gccggtcgcg
     3601 tgcgcgacca gaagccggca gtacaggctc atcctgcgga cgtgcgcccc ggtctcgtcg
     3661 tcgtgcagct cactggcgag caccaaccgg tggatcgtct cgcgtcgcga ctcgcgcagg
     3721 gcctgatggc tgcgctcgag ctggagcgtc gactgccaca gctcggaggt gcgcgccgcg
     3781 acgagctcct cgaggcgcag gtggtgattc ctgttgtcga tcgtgagctg ccggcgggtc
     3841 agcgcgttga acacgcagac gagcatctcc tcgacgtcga gcggcttcct caggtacccg
     3901 tagaccccca cgcggacggc ggcctcgccg acctcgcggc tccccttgcc ggtgagcatg
     3961 atcgtcccgg tgtccggaca cctcgcctgg acgtgctgca ggaggtcgag tcccgacccg
     4021 cccggcatgt cgaaatc
//
LOCUS       DASQDO010000170         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_201333, whole genome shotgun sequence.
ACCESSION   DASQDO010000170 DASQDO010000000
VERSION     DASQDO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201333"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="ribD"
                     /locus_tag="VEV43_04990"
     CDS             complement(<1..913)
                     /gene="ribD"
                     /locus_tag="VEV43_04990"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYP22907.1"
                     /translation="MRDDERYMARALELASTPAFASPNPRVGAVVVRDGAVLGEGAHE
                     GPGTPHAEALALSGIDARGATVYVSLEPCNHHGRMPPCAPALAEAGVARVVAAMEDPD
                     PRVAGSGFEVLRAAGIDVEVGLLRDRAERLNRAYLHHRRTGRAFVSLKLALTLDGRMT
                     AADGSSQWITGPEARRIVHARRVECDAVMVGAGTVLADDPQLTARDVPAPRQPLRVIV
                     DAAGRVPASAAALGDGSVVATTAAAPHETQIAWKETGAEVLVLPEESGGVDLGALLDA
                     LGSRNVVEVLCEGGPRLASSLVRDDLVG"
     regulatory      complement(995..1133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1176..2168)
                     /locus_tag="VEV43_04995"
     CDS             complement(1176..2168)
                     /locus_tag="VEV43_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP22908.1"
                     /translation="MIARRELVLVADDDQDIVRFVEVNLRLEGYEVATAHDGEQALQG
                     AYDLMPDLVLLDVMMPKIDGFEVCQRLRGDARTKHMSVIMLTAKSLSADKVVGLTAGA
                     DDYMIKPFDPMELVARVKSALRRSREMRAVNPLTQLPGNVQVQEEVAKRVALGAPFAL
                     MYIDLDNFKAFNDYYGFLRGDEAIKLLTKCASDALAKHGGPGCFLGHVGGDDFVAITI
                     PDVAELAAKEIIECWDDKIAELYDPDDLGRGFIEVKDRQDQMHRYGPTTVSIGIATNT
                     RRPINSHWEASEIAAEMKQFAKRDPRSSYAIDRRRDEDGDPTPEAARTNTGSRA"
     gene            complement(2165..2845)
                     /gene="rpe"
                     /locus_tag="VEV43_05000"
     CDS             complement(2165..2845)
                     /gene="rpe"
                     /locus_tag="VEV43_05000"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYP22909.1"
                     /translation="MKRVQIAPSILTADFGRITEEVSSVAPVVDWFHLDVMDGHYVDN
                     LTFGASTVAAIDRAVDTPLHVHLMIENPAKFAPVFAEAGADRISFHPEVVDDPAAVVD
                     LIREAGAEAGVAVHPDSDLGWAKRLLDGVAVVLMMTVRPGFGGQKFLAEVVPKIAEAR
                     KLVDQAGARADIEVDGGVNLETIAQPVGAGADVLVTGSAVYDGRDPVAAAIRIRERLD
                     MLAKEGDR"
     gene            complement(2842..>3316)
                     /locus_tag="VEV43_05005"
     CDS             complement(2842..>3316)
                     /locus_tag="VEV43_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYP22910.1"
                     /translation="TEELAVEPSDTAGSLGERMAELGAPLLVDTITRYASGELQPVEQ
                     DDALATYAPKITTEEARVDWKQPAERISNLVRGLNPAPGAWTELDGKRLKVWEVRHAP
                     TALEPGALEMLGSALLAGTSHTAVELADVQLAGKKRMSGGELARGLRLTPESRLS"
BASE COUNT          466 a         1158 c         1155 g          537 t
ORIGIN      
        1 gtccgacgag gtcgtcccgg acgaggctcg acgcgaggcg cggcccccct tcgcatagga
       61 cctcgacgac gttgcgggag ccgagggcgt cgaggagggc gccgaggtcg acgcctccgc
      121 tctcttccgg aagcaccagg acctcggccc ccgtctcctt ccacgcgatc tgggtctcgt
      181 ggggggcggc cgctgtcgtg gcgacgacgc tcccgtcgcc gagcgcagcc gcgctcgcgg
      241 gcactcgacc cgcggcgtcg acgatcacac gcaagggctg gcggggggcg gggacgtcgc
      301 gagcagtgag ctgcgggtca tccgcgagca cggtgcctgc gccgaccatg acggcgtcgc
      361 actcgacgcg gcgggcgtgg acgatgcgac gcgcctcggg gccggtgatc cactgcgacg
      421 acccgtccgc cgcggtcatg cgaccgtcga gcgtgagcgc gagcttcaac gagacgaagg
      481 ctcggcccgt gcggcggtgg tggaggtagg cgcggttcag gcgttcggcg cggtccctta
      541 gaaggccgac ttccacgtcg atgcctgcgg ctcgcagcac ctcgaagccg gacccggcga
      601 cgcggggatc ggggtcctcc atcgcggcga cgacccgcgc caccccggcc tccgccagcg
      661 ccggtgcaca cggcggcatg cggccgtgat ggttgcaggg ctcgagcgag acgtagacgg
      721 tcgcgccgcg ggcgtcgatg ccgctcagcg caagggcttc cgcgtgcgga gtgccggggc
      781 cctcgtgtgc gccctcgccg aggacggctc cgtcccggac gacgacggcc cctacgcggg
      841 ggttgggcga cgcgaaggcg ggcgtcgacg cgagctcgag ggcccgggcc atgtacctct
      901 cgtcgtcgcg cacgcgtctc ccttcatcct ccggggacga cggcgcagcg acgacggccc
      961 cgcacgtagg gtgcgggagc gcgcgaacgc tccgtcgcct cttctcccat ccggactatt
     1021 accgtcggtc ccggaattcc accgggtcaa cccggagcgc cgttcatgcg gctgcgccgg
     1081 ggtcgcggac tctcaccgcc ggtcgggaat cacaccctgc cccgaagagg ttcgacctaa
     1141 cgaaccgagt atagggccgg cccccgcaaa gggcgctagg ccctcgagcc tgtgttcgtc
     1201 cgagctgcct cgggcgtcgg gtcgccgtct tcgtcgcggc ggcggtctat cgcgtagctc
     1261 gagcgcgggt cccgcttcgc gaactgcttc atctcggccg cgatctcgga tgcttcccag
     1321 tggctgttga tcggccgccg cgtgttcgtc gctatgccga tcgagaccgt cgtcgggccg
     1381 tagcggtgca tctggtcctg gcggtccttc acctcgatga agccgcggcc gaggtcgtcg
     1441 gggtcgtaga gctcggcgat cttgtcgtcc cagcactcga tgatctcctt cgccgcaagc
     1501 tctgcgacgt cggggatagt gatcgccacg aagtcgtcac cgccgacgtg gccgaggaag
     1561 cacccgggac cgccgtgctt ggcgagcgcg tcgctcgcgc acttcgtcag gagcttgatc
     1621 gcctcgtccc cgcgcaggaa gccgtagtag tcgttgaacg ccttgaagtt gtcgaggtcg
     1681 atgtacatga gcgcgaacgg cgcaccgagc gcgacacgct tcgcgacctc ctcctggacc
     1741 tggacgtttc cggggagctg cgtcagtggg ttcaccgcgc gcatctcgcg cgaacgacga
     1801 agcgcagact tcacgcgcgc tacgagctcc atcgggtcga acggcttgat catgtagtcg
     1861 tcggcgccgg ccgtgaggcc gacgaccttg tcggccgaca gcgacttcgc cgtcagcatg
     1921 atcacggaca tgtgcttggt ccgggcgtcg ccgcgcagcc gctggcacac ctcgaagccg
     1981 tcgatcttcg gcatcatgac gtcgagcagg acgaggtcgg gcatgaggtc gtacgcgccc
     2041 tggagagcct gctcgccgtc gtgagcggtc gcgacctcgt aaccctccag ccggaggttg
     2101 acctcgacga accggacgat gtcctggtcg tcgtcggcca cgagcactag ctcgcgccgg
     2161 gcgatcatcg gtccccttcc ttcgccagca tgtccaatct ctctctaata cgtatggcgg
     2221 ccgcaacggg atcgcggccg tcgtagaccg cgctgcccgt gacgagcacg tctgccccgg
     2281 cccccacggg ctgcgcgatg gtctcgaggt tgacgccccc gtccacctct atatcggcac
     2341 gtgcgcccgc ctggtccacc agctttcgcg cttcggcgat cttcggaacc acctcagcga
     2401 ggaacttctg acctccgaaa ccgggacgta ccgtcatcat aaggacaact gcgacaccat
     2461 ccaagagtcg ttttgcccag ccgaggtcgc tgtcggggtg gacggccacg cccgcctcgg
     2521 cccccgcctc ccggatcagg tcgaccacgg cggccgggtc gtccacgacc tcgggatgga
     2581 aggagatccg gtccgccccg gcttcggcga acacgggcgc gaacttcgcg gggttctcga
     2641 tcatcaggtg gacgtgcagc ggtgtgtcga cggcccggtc gatggcggcc acggtcgagg
     2701 ctccgaacgt gaggttgtcg acgtagtggc cgtccatgac gtccaggtgg aaccagtcga
     2761 ccaccggcgc gaccgaggag acctcctcgg tgatccgccc gaagtccgcg gtgaggatcg
     2821 agggcgctat ctggacgcgc ttcacgagag cctcgactcg ggggtgaggc ggaggccgcg
     2881 ggcgagctct ccgccgctca tcctcttctt gccggcgagc tgcacgtcgg cgagctcgac
     2941 cgccgtgtgg gaggtgccgg ccagcagcgc ggacccgagc atctccaggg caccgggttc
     3001 gagggccgtg ggggcgtgcc gtacctccca gaccttcaat cgcttcccgt cgagctcggt
     3061 ccacgcgccg ggggcagggt tgaggccgcg cacgaggttc gagatccgtt ccgcaggctg
     3121 cttccagtcg acgcgcgcct cttccgtcgt gatcttcggc gcgtaggtcg cgagcgcgtc
     3181 gtcctgctcg acgggctgga gctcgcccga ggcgtagcgc gtgatcgtgt ccacgagcag
     3241 aggagcgccg agctccgcca tccgttcgcc gaggctcccc gcggtgtcgg acggctcgac
     3301 ggccaactct tcggtc
//
LOCUS       DASQDO010000171        14123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1058021, whole genome shotgun sequence.
ACCESSION   DASQDO010000171 DASQDO010000000
VERSION     DASQDO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14123
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058021"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VEV43_05010"
     CDS             <1..181
                     /locus_tag="VEV43_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYP22911.1"
                     /translation="KLSEVQREAIVLAYFGGRTYREVAQELEIPEGTAKTRLRDGLIR
                     LREALAPTQESGGWS"
     gene            172..897
                     /locus_tag="VEV43_05015"
     CDS             172..897
                     /locus_tag="VEV43_05015"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYP22912.1"
                     /translation="MVVTHDEMKSLIAPYVLGAVTPDEEREVRSHIMGCDECMREAEG
                     FSAPAAALALAVEEAPLPAGFADAVVARVASERPERAAAPSRVDGWFRWRKVLAGVAA
                     ASVVATIVLAGAFLAQRAQVRRYEDAVPPLVHGEGMRLAGAGGAVARMVPTADGATLF
                     ATGLDEAPERHTYQLWLMECTDPEVIETCDPTSAGTFDVSGGIAVLETRTSLEGYDRA
                     AVTVEPDGGSDGPTTLPVIDSAA"
     gene            932..2446
                     /locus_tag="VEV43_05020"
     CDS             932..2446
                     /locus_tag="VEV43_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP22913.1"
                     /translation="MRAGKDPYMPVKRRVVYVVVDGMSTTAFEQAIASGRAPAFSFLR
                     DRARYVRDSVAVFPTITPCATASLVTGETPARHRIPGMCWYDRDAQRFVNYGQSPRAA
                     VVEGMSQIVRDVIENLNSHHLSPEVRTIHEQLHDRGLTTASINFMLFRGPFEHELETN
                     LWQKLLFRRHLPETIPGPKEHYFADVVSGPAGGCQKFPKGRKIEHRIHATDGWAACVT
                     RELLEKNAADMILFYLHENDHMSHKHGPSSQIDSLEAADEHLAYVLESLGSWERTAEE
                     VGFVVTSDHSQSPIADADDHVCDLGEILDGFSQVTPGRGKERFGDSDVATAGNGRVGF
                     VYLNEARKDALRRPVVTALLEHPGTDQVMWVDGETCHVETNRGSLTFREAQGYGAVVD
                     ERGNKWEYEGDLEAVGGVVEEDGIRTPEYPLALWRIMCALRLDRMGDVVVTPKLTYEY
                     NDLAGGNHRGGGDHASLHAQDSLVPFLSTLDDPPLRPSTVDVVPHIVKHFESLR"
     gene            2446..3294
                     /locus_tag="VEV43_05025"
     CDS             2446..3294
                     /locus_tag="VEV43_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HYP22914.1"
                     /translation="MRYLVIGNPASSGFSEDVRAAVDAEIPDAQWFEPGKGDLVEAAA
                     GADVVVVAGGDGSLNHAVNELEARLGELRFGLVPVGTGNDFARTAGIPADPAEAARLV
                     VSGSEREIDVARASGPGVNRLFVNACMGGFPVQVNEAIDEDTKERLGPLAFWVGGAKA
                     AADLRRSKVVVNGIELDGCVAAGVGNGRTCGGGIPVWPDADPSDGSLDACALAAPNVV
                     SAVKVLAKVRSGEHGDLDEVRTTRGPRVTIEADPPIELNVDGELLGLKTPATFEICGT
                     LRLIVR"
     gene            complement(4046..4444)
                     /locus_tag="VEV43_05030"
     CDS             complement(4046..4444)
                     /locus_tag="VEV43_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22915.1"
                     /translation="MDEREFATLLQLIHDRLREEGFADLSAGLLAQTALDEDRPSGHV
                     LRHLRLLRDEWSLRSRTSVMAVMGYMNEVVRPASREVIRSPHIALGELEAGFYENESV
                     DMMEAPDLGDVIAALDRLIGDLAEDRETWD"
     gene            4522..5307
                     /locus_tag="VEV43_05035"
     CDS             4522..5307
                     /locus_tag="VEV43_05035"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYP22916.1"
                     /translation="MKVRDALIAALRRDPDLVPKVKGSLRERIDDEGRVATVLQAAEA
                     LEPIIADALRKRPSRIKRLGLKSAQLLAGLAAEDDESARRLANIATGSTVGIVFADVA
                     GFTTMTERLGDRGAIEVLTRLERIVERELVRTKGEIVKQLGDGFLLAFPSASQAVRGA
                     LALRDAVGRERANDPAFEVKLRIAVHAGEPLITGDDLLGHDVNLAARLLDHCKPDRVV
                     VSEAAKELAERRLKSVDFTSRRAVKIRGLSGRTTLYTARPAPS"
     gene            complement(5304..5903)
                     /locus_tag="VEV43_05040"
     CDS             complement(5304..5903)
                     /locus_tag="VEV43_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HYP22917.1"
                     /translation="MRRFRRVLLLVSGVLLVLLAYPVWLAWRIWDQSHNDEVRAADAI
                     VVLGAAQYDGRPSPVLKARLDHALYLYEEDLSPTLIVTGGKREGDRFAEAEVGAMYLE
                     DEGVPPEAILAEEQGTTTLESLEQVHELSREEGIDTVLLVSDPLHSERVKTMAQDLGF
                     EEAWASPASYVELNRTRRTKAQELLHEIASLAAYELLNR"
     gene            complement(6022..6726)
                     /locus_tag="VEV43_05045"
     CDS             complement(6022..6726)
                     /locus_tag="VEV43_05045"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HYP22918.1"
                     /translation="MDVRLKILRYNPETDSAPHFEEYTVKAEPMDRLLDCLHAVKWYH
                     DSSLALRRSCAHGICGSDAMVINGVNALACKLLVKNLKQPITVEPIRGLPVKRDLIVD
                     MEPFFKGYRAVKPWLITDDEEKDEARERIQSPEDRARYDDTTKCILCAACTTSCPIFW
                     SDEEYVGPAAIVNAHRFIFDSRDRGGRERLEILSRKDGAFKCRTAFNCTEACPRGIKV
                     TKAIQEVKRASLFQSL"
     gene            complement(6726..8462)
                     /gene="sdhA"
                     /locus_tag="VEV43_05050"
     CDS             complement(6726..8462)
                     /gene="sdhA"
                     /locus_tag="VEV43_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HYP22919.1"
                     /translation="MARFHKYDAVIVGAGGAGLRAALEAGKRCKTAVVSKLYPTRSHT
                     GAAQGGMCAALANVEEDSWEWHAFDTVKGSDFLADQDAVDIMCKEAVEAVLELERMGL
                     PFNRTPDGKIDQRRFGGHTRNHGEAAVKRACYAADRTGHMILQTLYQQCNKEGVEFYD
                     EFFVLDLIMVDGSCAGIVAYEIATGEIHVFHAKSVLFATGGFGKMFKITSNAHTLTGD
                     GPGVVFEQGLPLEDMEFFQFHPTGLYGLGILLSEAARGEGGILRNSDGERFMERYAPT
                     IKDLAPRDMVSRAEFFEIQEGRGAGPKKDYILLDLTHLDAEVIEAKLPDITEFARTYL
                     NVEPLTEGVPIVPTAHYAMGGIPTNVNAEVLSNHWGDVVPGFYAAGECACVSVHGANR
                     LGTNSLLDIVVFGRRGGLNMAEYAAAHKHLDIPDEPHLRAERKLASFYETGGVESTAH
                     IRKTLQDDMMDNASVVRTDESLGKALANIQALKKRYAQAPVRDRAKVYNTEVVEHFEL
                     GFLLDMAEALVVSARARTESRGGHYREDHKMREDDHWMKHTFATRASDGAIRLDYKDV
                     TLGRYEPVERKY"
     gene            complement(8467..8886)
                     /locus_tag="VEV43_05055"
     CDS             complement(8467..8886)
                     /locus_tag="VEV43_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HYP22920.1"
                     /translation="MATVEEVYKKHKPKAFSADRRPSSFEVWSWFFMRLSGIVLLFLV
                     LIHLFVMHIVDEGVERVNFAFVAERWDNVGWKTFDWTMLFLSLLHGVNGLRIIIEDYV
                     HNSAWRISIKGVLYVTTVILMVMGTAVIVTFDPNVGG"
     gene            8931..10499
                     /locus_tag="VEV43_05060"
     CDS             8931..10499
                     /locus_tag="VEV43_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYP22921.1"
                     /translation="MSQERRTDSGIEIQPLYGPGGPEPELGAPGEWPFTRGVYKGMYR
                     DRLWTMRQYAGFGSAEETNERFRYLLRAGQTGLSVAFDLPTQMGYDSDHPKAQAEVGR
                     VGVAIDSLADMELLFREIPLGEVSTSMTINATASILLLLYELVAEKQGADPARITGTT
                     QNDILKEYAARGTYIYPPRPSMRIITDLFAYCRDRLPRWNTISISGYHMREAGCTAAQ
                     EIAFTIADGIAYVKAATEAGLDVDDFAPRLSFFFACHMNFFEEVAKFRAARRLWAKVM
                     KERFGATNPKSLMLRFHTQTGGATLTAQQPENNIVRTALEALAAVLGGTQSLHTNSFD
                     EALALPSEKAATIALRTQQVIGYEAGVADTVDPLAGGWFVESLTDELEGLATEYLERI
                     DAMGGAVEAIESGWMKNEIEDAAYRVTQGIESGDRVVVGVNKFVAESEEDVEIHSLDP
                     ELERRQVERTQKVRAERDQAAVDAALKSLGEAARGDDNLLYPMREALAAYATLGEVSD
                     VLREVFGEYEPKSL"
     gene            10496..11164
                     /gene="npdG"
                     /locus_tag="VEV43_05065"
     CDS             10496..11164
                     /gene="npdG"
                     /locus_tag="VEV43_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HYP22922.1"
                     /translation="MTVDKVAVIGGTGDEGFGLAFRWARAGIDVVIGSRDAGRAEEAA
                     GRVKELVPDANVSGAENSAAVASSELVMVTVPFGGQAAIYRSIAESVRPDSVVVDGTV
                     PVAASVGAKVTHTLGVWEGSAAQQAAAFLPKGTRTCAAFHSLAAVALNDPDYEMEGDV
                     LVCGPKSAKPVVQELVEAIPGLRFVDAGPLENARLVEPITALLIGINHRYETDRSGIR
                     ITGI"
     gene            complement(11161..11751)
                     /locus_tag="VEV43_05070"
     CDS             complement(11161..11751)
                     /locus_tag="VEV43_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22923.1"
                     /translation="MDGRKAVAAGLFVLATAGGCSPWGPLFVAGGLGGFASEVRSVGA
                     PETERVPRPKLDEVFRDGRYSFRPAPAAVRRATVRTMEKDPLAETSIQKVVVKAASEA
                     SGGTEPLVIGIVWTPAAARSPNGWEDFLTGYTGDGDVELARGRLSGTNVAYADQRAAR
                     VVLVDYSTRVTLMIMGPPRDPLTPLKDVARYLLRSR"
     gene            11785..13236
                     /gene="pyk"
                     /locus_tag="VEV43_05075"
     CDS             11785..13236
                     /gene="pyk"
                     /locus_tag="VEV43_05075"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYP22924.1"
                     /translation="MMRRAKIVATAGPASRSREVLRELVGAGVDVLRLNLAHPSPEAH
                     AQAARIAREEASAVGRVVAVLADLPGPKIRTGPIAGGEVVLEPGATLYLTTEQIEGDA
                     RRGSTTLAALPEIVTRGDQVFLADGEVILEVVSADGTDVRTDVVRGGILRSRKGMHLP
                     QHEGRIEAFTEKDRGSLRDAVAMRADLVGLSFVRDADDLRRVRAELPDEDPPLLVAKI
                     ETRSAVENLAAIVDEADAVMVARGDLGIQTSLSELPLLQKRIIEACNCAGKPVITATQ
                     MLESMTRAPLPTRAEVADVANAVLDGTDALMLSEETAVGDRPVEVVKVMDGIITAAER
                     SHREHQSPASDDLHDDDPVSWATAHAGVQAATDLKAAAILCPTRTGSTALKVAAFRPP
                     MPVVGISPHSHPRAMLALAWGVVPLGSADYATASAEGAAGIPGERRKNDIDVGVRVAL
                     AAGTVVAGDVVVVVAGSAAARPGGTDLVRVMTC"
     gene            complement(13241..>14123)
                     /locus_tag="VEV43_05080"
     CDS             complement(13241..>14123)
                     /locus_tag="VEV43_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP22925.1"
                     /translation="GDPCLVETSGGNVSADHVVVASHYRFLYRSMMFPRVHPSRSYAM
                     VGPVSGTVPEGMYLSTEPAWSVRPVRVGDETLVLTGGAGHTVGQHYDTEARYEELERW
                     SREKLGMTEVRYRWSTQDGTSVDTLPYIGRLTPVSRRAYTATAFRKWGMTNGTLAGMI
                     LADLITETPNEWASLYDPHRATLKASAPKFAKENVKVAGHWLRDRLSHPQSGVPGDLA
                     PGEAAVVGTAPGQVAAYRDDAGELHKVSAVCTHLGCIVSWNNAETTWDCPCHGSRFDV
                     DGKVIQGPAVKDLEPKD"
BASE COUNT         2255 a         4848 c         4847 g         2173 t
ORIGIN      
        1 gaagctcagc gaagtgcaac gggaagcgat cgtgctcgcg tatttcgggg gccgaacgta
       61 ccgcgaggtc gcgcaggagc tcgagatccc cgaaggcacc gcgaagaccc ggctgcgtga
      121 cggcttgatc cgtttgcgcg aggctctggc cccgacacag gagagcgggg gatggtcgtg
      181 acgcacgacg agatgaagtc gctgatcgcg ccctacgtgc tcggtgcggt gacgcccgac
      241 gaggagcgcg aggtccgctc gcacatcatg ggctgcgacg agtgcatgcg cgaggcggag
      301 ggcttctcgg cccccgccgc ggccctggct ctcgcggtcg aagaggctcc ccttcccgcc
      361 ggtttcgcgg acgccgtcgt cgcccgggtc gcgagcgaac ggccggagcg cgccgccgcc
      421 ccctcccgcg tcgacggatg gttccgctgg aggaaggtct tggccggcgt ggccgcggcc
      481 tccgtcgtcg ccacgatcgt cctggcgggc gcgttcctcg cgcagcgtgc gcaggtgcgc
      541 cgctacgaag acgcggtgcc accgctcgtc cacggcgagg gcatgaggct cgccggagcg
      601 ggcggggccg tcgcgcggat ggtccccacg gccgacggag cgacgctctt cgcgaccggc
      661 ctcgacgagg cccccgagcg gcacacgtat cagctctggt tgatggagtg caccgatccc
      721 gaggtcatcg agacgtgcga cccgacgagc gcggggacgt tcgacgtctc gggcgggatc
      781 gcggtgctcg agacgcggac gagcctcgag ggctacgacc gggccgcggt caccgtcgag
      841 ccggacggtg gctccgacgg cccgacgacc ctgccggtga tcgactcggc tgcctgagca
      901 agcaatcacg ctccgtaagg gattagatgc ggtgcgtgcg gggaaagacc cgtacatgcc
      961 ggtcaagaga cgagtcgtct acgtcgtcgt cgacgggatg agcacgactg cgttcgagca
     1021 ggcgatcgcg tccggccggg cccccgcttt ctcgttcctg agagaccgcg cccggtacgt
     1081 ccgcgactcg gtcgcggtct tcccgacgat cacgccgtgt gcgaccgctt cgctcgtgac
     1141 gggcgagacg ccggcccgtc accgcatccc gggcatgtgc tggtacgacc gggacgcgca
     1201 gcgattcgtg aactacgggc agagccctcg cgcggcggtg gtcgagggca tgtcgcagat
     1261 cgtgcgagac gtcatcgaga acctcaactc gcaccatctg agccccgagg tccggacgat
     1321 ccacgagcag ctccacgacc gaggcctcac gaccgcgtcg atcaacttca tgctgttccg
     1381 ggggccgttc gagcacgagc tcgagacgaa cctgtggcag aagctgttgt tccgccgcca
     1441 cctccccgag acgatcccgg gcccgaagga gcattacttc gccgacgtcg tgagcggtcc
     1501 cgcgggaggc tgtcagaagt tcccgaaggg ccgcaagatc gagcaccgca tccacgcgac
     1561 cgacgggtgg gccgcatgcg tcacgcgcga gctcctggag aagaacgcgg ccgacatgat
     1621 cctgttctac ctgcacgaga acgaccacat gtcgcacaaa cacggccctt cgagccagat
     1681 cgacagcctc gaggccgcgg acgagcatct cgcgtacgtg ctggagtcgc tcggctcgtg
     1741 ggaacggacc gcggaggagg tcggcttcgt cgtgacgtcg gaccactcgc agagcccgat
     1801 cgccgacgcc gacgaccacg tctgcgacct cggcgagatc ctcgacgggt tcagccaggt
     1861 cacgccgggc cgcggcaagg agcgtttcgg cgactccgac gtcgcgaccg ccggcaacgg
     1921 ccgcgtcggt ttcgtgtatc tgaacgaggc gcgcaaggac gcgcttcgcc ggcccgtcgt
     1981 aaccgctctt ctcgagcatc cgggcaccga ccaggtcatg tgggtcgacg gcgagacctg
     2041 ccacgtcgag accaaccgcg ggagcctgac gttccgcgag gcgcagggct acggcgcggt
     2101 cgtcgacgag cgcgggaaca agtgggagta cgagggcgac ctcgaggccg tcggcggcgt
     2161 cgtcgaggaa gacggcatcc ggacgccgga gtaccccctc gcgctgtggc ggatcatgtg
     2221 cgccctccgg ctcgaccgca tgggcgacgt cgtcgtcacg ccgaagctga cctacgagta
     2281 caacgacctc gccggcggga accatcgcgg cggtggggac cacgcttcgc tccacgcgca
     2341 ggactcgctc gtgccgttcc tgtcgacgct ggacgacccg ccgctaaggc cgtcgacggt
     2401 cgacgtcgtg ccgcacatcg tcaaacactt cgagtcgctg cgttagtgcg ctacctcgta
     2461 atcggcaacc ccgcgtcgag cggcttctcc gaagacgtcc gggctgccgt cgatgccgag
     2521 atccccgacg cgcagtggtt cgagcccggc aagggtgacc tggtggaggc agcggccggc
     2581 gcggacgtcg tcgtcgtagc cggtggggac ggctcgctga accacgcggt gaacgagctg
     2641 gaggcgcggc tcggcgagct ccggttcggc ctggtcccgg tggggaccgg caacgacttc
     2701 gcacgcacgg cggggatccc ggccgacccc gcggaggccg cacgcctcgt cgtctccggg
     2761 tcggagcggg agatcgacgt ggcccgcgcg agcgggcccg gagtcaatcg cttgttcgtc
     2821 aacgcgtgca tgggtggctt cccggtccag gtgaacgagg cgatcgacga ggacacgaaa
     2881 gagcgtctgg gccccctggc gttctgggtc ggtggggcaa aggccgcagc ggacctccgt
     2941 cgctcgaagg tcgtggtcaa cgggatcgag ctggacggct gcgtagccgc cggcgtcggc
     3001 aacggaagga cgtgcggggg cgggatcccg gtgtggcccg acgccgaccc gtccgacggg
     3061 tcgctcgacg cctgcgccct ggcggcgccc aacgtcgtgt cggccgtcaa ggtcctggcg
     3121 aaggtccggt ccggtgagca cggggacctg gacgaggtgc gcacgacccg cggcccccgc
     3181 gtcacgatcg aggcggaccc gccgatagag ctgaacgtcg acggcgagct gctgggcctg
     3241 aagaccccgg cgaccttcga gatctgcggg acgctgcgcc tgatcgtgcg ctaggcgcgg
     3301 tcagcacctt tctgtgcccc atccggccgc atggcgagcg tcgtgtgaca aggaagcccc
     3361 tctaggatcg aacccttcta cggtgcggag tggtacacga agtcgatgcc gaagacatcg
     3421 tgaggcggtt ctagcgaaac tatgttgtcg tcgcttgcgt acaccaacag gcacgacgga
     3481 gggttggccg gcacatctcg ccgaggccat cggcgaaagc ttgtcggaat tgggggaacc
     3541 aagaggctca tctctcgacg cactcccaac acctgtcgaa gtacgtcctt tccaagaaga
     3601 ccactcttgc actcgacgcc tatcaatacc tggtcgtgcg gaggtcggcc gtgttcgccc
     3661 tccggaacga cgacgatgtc gagctcgtgg aagtccgctc gagcaggcgg acccgacgct
     3721 ccgcggacgt cgtgactcag agtgaggaat tccacgtccg tccaaacttc gagagtccga
     3781 ccatgcgggt gccgtgcctc gaaatacgga tagctccgat tgatcggtcc cggcgaggtc
     3841 tttagaaccg ctcgctgact gctaacgagc cgaaccaaat atccttcccg ccgaagtcga
     3901 gccagcacgt cgacgagaac ccacgcctcg tagaccttgc ccgcgccaaa tgacgaagcc
     3961 atgaggggat gcccagtccg gaccccccgc ctgtagccgt cgaagattcg ttctatgcgc
     4021 tggcgcgctt ccgcgaggtc caccgctaat cccaggtttc tcggtcttcg gcgagatctc
     4081 caatgaggcg gtccagagct gcaatgacgt cgcccaagtc tggcgcctcc atcatgtcga
     4141 cactctcgtt ctcgtagaag ccggcctcga gctcgccgag cgcgatgtgg gggctgcgga
     4201 tgacttcccg cgaagccggg cggacgactt cgttcatgta gcccatgacg gccatcaccg
     4261 acgtccgcga tcgtagcgac cactcgtccc ggagcaaccg cagatgcctc aggacatgcc
     4321 cggaggggcg atcctcgtcg agagcggtct gagcgagtag cccagcgctg aggtcggcga
     4381 aaccttcctc gcgcagacgg tcgtggatga gctggaggag tgtcgcgaac tctctttcgt
     4441 ccacgacaag agtgtgacac gcggtggtct cggtcgttac tcgaaacctt cccgttacat
     4501 cccgtgagta acgtgtcctc cgtgaaggtc cgcgacgctc tgatcgccgc gctgcggcgg
     4561 gaccccgacc tcgtccccaa ggtcaagggc tcgctacgcg agcgcatcga cgacgagggc
     4621 cgcgtcgcga ccgtcctgca ggccgccgag gccctcgagc cgatcatcgc cgacgccctc
     4681 cgcaagcggc cctctcggat caagcggctc ggcctcaaga gcgcgcagct cctcgccggc
     4741 ctcgcggccg aggacgacga gtcggcccgc cgcctggcga acatcgcgac ggggtcgacc
     4801 gtcgggatcg tcttcgccga cgtcgcgggt ttcacgacga tgaccgagag gctcggcgac
     4861 aggggagcga tcgaagtcct cacgcgactg gagcgcatcg tcgaacgcga gctcgtccgg
     4921 acgaagggcg agatcgtgaa gcagctcggc gacggcttcc tgctggcgtt cccctcggct
     4981 tcgcaggcgg tccggggtgc gctggcgctg cgcgacgccg tcggccggga gcgcgcgaac
     5041 gacccggcct tcgaagtgaa gctgaggatt gccgtccacg cgggcgagcc gttgatcacc
     5101 ggcgacgacc tgctgggcca cgacgtcaac ctggcggcgc gtctcctcga ccactgcaag
     5161 ccggaccggg tcgtggtctc ggaggcagcg aaggagctcg ccgaacgccg cctgaagtcc
     5221 gtcgacttca cgagccgccg ggcggtgaag atccgcggcc tcagcggccg gacgacgctc
     5281 tacaccgccc ggccggcccc ctcctagcgg ttcagcagct cgtacgccgc cagcgacgcg
     5341 atctcgtgca gcagctcctg cgccttcgtg cgacgcgtgc ggttcagctc gacgtacgac
     5401 gcagggctcg cccacgcctc ctcgaagccc aggtcctgcg ccatcgtctt cacgcgctcc
     5461 gagtgcaggg ggtccgacac gaggagcacg gtgtcgatgc cctcctcccg cgacagctcg
     5521 tggacctgct cgaggctctc gagcgtcgtc gtcccctgtt cttcggccag gatcgcctcg
     5581 ggcggcacgc cctcgtcctc gagatacatc gcgccgacct cggcctcggc gaagcgatcg
     5641 ccctcccgct tgcccccggt gacgatgagg gtcggcgaga ggtcctcctc gtagaggtag
     5701 agcgcatggt cgagccgcgc cttgagcacc ggcgacggcc gcccgtcgta ctgcgcggcc
     5761 cccaggacga cgatcgcgtc ggcggcccgg acctcgtcgt tgtgggactg gtcccagatg
     5821 cgccacgcga gccagaccgg ataggcgagc agcacgagaa gcacgcccga gacgaggagc
     5881 aggacgcggc ggaagcggcg catggcccga ggttagatca ggtagcgacc caacggtcgg
     5941 agcggatacc caccttcccg agaggtgtca gacatgtcag acacggctgt catgtctgcc
     6001 gtgtctgccg caccccggcc cctacaggga ctggaacagc gatgcccgct tcacctcttg
     6061 gatcgccttc gtcaccttga tcccgcgagg gcaggcctcc gtgcagttga acgccgtccg
     6121 gcacttgaag gcgccgtcct tgcggctcag gatctcgagc cgctcccggc ccccgcgatc
     6181 gcgcgagtcg aatatgaaac ggtgcgcgtt cactatcgcc gccgggccca cgtactcctc
     6241 gtccgaccag aagatcgggc acgacgtcgt gcacgccgcg cagaggatgc acttcgtcgt
     6301 gtcgtcgtag cgggcgcggt cctcgggcga ctggatgcgc tcgcgcgcct cgtccttctc
     6361 ctcgtcgtcg gtgatcagcc acggcttgac cgcccggtat cccttgaaga acggctccat
     6421 gtcgacgatg aggtcgcgct tgaccgggag gccgcgaatc ggctcgaccg tgatcggctg
     6481 cttgaggttc ttcacgagca gcttgcacgc cagcgcgttc acgccgttga tcaccatcgc
     6541 gtcggagccg cagatcccgt gcgcgcacga gcgccgcagc gccagagacg agtcgtggta
     6601 ccacttcacc gcgtgcaggc agtcgagcag ccggtccatc ggctcggcct tcaccgtgta
     6661 ctcctcgaag tgcggagccg agtcggtctc ggggttgtag cgcaggatct tcagccggac
     6721 gtccatcagt acttcctctc gacgggctcg tagcgaccga gcgtgacgtc cttgtagtcc
     6781 agacggatcg cgccgtcgga cgcacgtgtc gcgaacgtgt gcttcatcca gtggtcgtcc
     6841 tcgcgcatct tgtggtcctc gcggtagtgg ccgccgcggc tctcggtccg ggcccgcgcc
     6901 gacaccacga gcgcctcggc catgtccagc aggaagccca gctcgaagtg ctcgacgacc
     6961 tcggtgttgt agaccttcgc gcggtcccgt acgggggctt gggcgtaacg cttcttgagc
     7021 gcctggatgt tcgcgagcgc cttgccgagc gactcgtccg tccgcacgac cgaggcgttg
     7081 tccatcatgt cgtcctgcag cgtcttgcgg atgtgcgccg tcgactcgac gccgccggtc
     7141 tcgtagaacg acgcgagctt gcgctcggct cgcagatgag gctcgtcggg gatgtcgagg
     7201 tgcttgtgcg ccgcggcgta ctcggccatg ttcaggcccc cgcgccgccc gaacacgacg
     7261 atgtcgagca gcgagttcgt ccccagccgg ttcgcgccgt gcacggacac gcacgcacat
     7321 tcaccggccg cgtagaagcc cgggacaacg tcgccccagt ggttcgagag cacctcggcg
     7381 ttgacgttcg tcgggatccc gcccatcgcg tagtgagcgg tgggcacgat cgggacgcct
     7441 tcggtcagcg gctcgacgtt caggtacgta cgggcgaact cggtgatgtc cggcagcttg
     7501 gcctcgatga cctccgcgtc gaggtgcgtg aggtcgagga ggatgtagtc cttctttggc
     7561 ccggcccccc gcccctcctg gatctcgaag aactcggccc gcgacaccat gtcgcgcggc
     7621 gcgaggtcct tgatcgtcgg cgcatagcgc tccatgaagc gctcgccgtc ggagttgcgc
     7681 aggatgccgc cctcgccgcg ggccgcctcg gacaaaagga tcccgagccc gtacaggccc
     7741 gtgggatgga actggaagaa ctccatgtcc tcgagcggca ggccctgctc gaagaccacg
     7801 ccgggcccgt cgccggtcag cgtgtgcgcg ttcgacgtga tcttgaacat cttgccgaag
     7861 ccgccggtgg cgaacaggac ggacttggcg tggaagacgt ggatctcgcc ggtggcgatc
     7921 tcgtacgcga cgatccccgc gcacgaaccg tcgaccatga tgaggtcgag gacgaagaac
     7981 tcgtcgtaga actcgacgcc ctccttgttg cactgctggt agagcgtctg gaggatcatg
     8041 tgaccggtgc ggtcggcggc gtagcaggcc cgcttgacgg cggcctcgcc gtgattgcgg
     8101 gtgtgcccgc cgaaccggcg ctggtcgatc ttgccgtcgg gggtgcggtt gaaggggagg
     8161 cccatgcgct cgagctcgag caccgcctcg acggcctcct tgcacatgat gtcgacggcg
     8221 tcctggtcgg cgaggaagtc cgaacccttg acggtgtcga acgcgtgcca ctcccaggag
     8281 tcctcctcga cgttcgcgag ggccgcgcac atcccgccct gagcggcccc cgtgtgcgaa
     8341 cgcgtcgggt agagcttcga gaccacggcg gtcttgcacc tcttgccggc ctcgagggcg
     8401 gccctcagcc cggccccccc ggctccgact atgacggcgt cgtacttgtg gaagcgggcc
     8461 acccgctcag ccgcccacgt tcgggtcgaa cgtcacgatc acggcggttc ccatgaccat
     8521 gaggatcacg gtggtcacgt acaggacgcc cttgatcgag atcctccacg ccgagttgtg
     8581 gacgtagtcc tcgatgatga tccggaggcc gttcacgccg tggagcaggc tcaggaacag
     8641 catcgtccag tcgaaggtct tccagccgac gttgtcccag cgctcggcga cgaatgcgaa
     8701 gttcacccgc tcgacgccct cgtcgacgat gtgcatcacg aagaggtgga tcagcacgag
     8761 gaacaggagc acgatcccgg acagccgcat gaagaaccag gaccacactt cgaacgacga
     8821 aggccggcgg tcggcggaga aagccttcgg cttgtgcttc ttgtagacct cttccacggt
     8881 cgccatgggg ccgcatccta accgtccgaa tgaggctcta gcattgcaac gtgagccaag
     8941 aacgccgtac cgattccggc atcgagatac agccgctgta cggccccggc ggccccgagc
     9001 ccgagctcgg cgcgcccggc gaatggccct tcacccgggg cgtctacaag ggcatgtacc
     9061 gcgaccgcct ctggacgatg cggcagtacg cgggcttcgg gagcgccgag gagaccaacg
     9121 agcgcttccg ctacctcctg cgcgccggcc agaccgggct ctcggtggcg ttcgacctcc
     9181 cgacgcagat gggctacgac tccgaccacc cgaaggcgca ggccgaggtg ggccgggtcg
     9241 gggtcgcgat cgactcgctg gcggacatgg agctgctgtt ccgggagatc ccgctcggcg
     9301 aggtctcgac ctcgatgacg atcaacgcga cggcgtcgat cctgctgctc ctctacgagc
     9361 tcgtcgccga gaagcagggc gccgaccctg cgcgtatcac ggggacgacc cagaacgaca
     9421 tcctcaagga gtacgcggca cgcggcacct acatctatcc gccccggccc tcgatgcgga
     9481 tcatcaccga cctgttcgcc tattgccggg accggctccc gcgctggaac acgatctcga
     9541 tctccggcta ccacatgcgc gaggcaggat gcacggccgc gcaggagatc gccttcacga
     9601 tcgccgacgg gatcgcgtac gtgaaggcgg cgaccgaggc cgggctcgac gtcgacgact
     9661 tcgccccgcg gctctcattc ttcttcgcgt gccacatgaa cttcttcgag gaggtggcga
     9721 agttccgagc cgcccggcgg ctgtgggcga aggtcatgaa ggagcgtttc ggcgcgacga
     9781 acccgaagtc gttgatgctg cgcttccaca cgcagaccgg gggcgcaacg ctcaccgcgc
     9841 agcagcccga gaacaacatc gtgcggacgg ctttagaggc cctcgccgcg gtgctcggcg
     9901 ggactcagtc gctgcacacg aactccttcg acgaggcgct ggcgctcccg agcgagaagg
     9961 ccgcgacgat cgccctgcgg acccagcagg tcatcggcta cgaggccggg gtggcggaca
    10021 ccgtcgaccc gctcgcgggc gggtggttcg tcgagtcgct gacagacgag ctcgagggcc
    10081 tggcgaccga gtacctcgaa cggatcgacg cgatgggcgg ggccgtcgag gcgatcgagt
    10141 cgggctggat gaagaacgag atcgaggacg cggcctaccg cgttacgcag gggatcgagt
    10201 ccggagaccg cgtggtcgtc ggcgtgaaca agttcgtagc ggagtccgag gaggacgtcg
    10261 agatccactc gctcgacccc gagctcgaac gccggcaggt cgagcggacg cagaaggtgc
    10321 gcgccgagcg cgaccaggcc gccgtcgacg cggcgctgaa gtcactcggc gaagccgcac
    10381 ggggcgacga caacctcctg tacccgatga gggaggcgct ggcggcgtac gcgacgctcg
    10441 gcgaggtctc ggacgtgctg agggaagtct tcggcgagta cgagcccaaa tcgctgtgac
    10501 ggtcgacaag gtcgccgtca tcggcggcac cggcgacgaa ggcttcggcc tcgctttccg
    10561 ctgggcccgc gccggcatcg acgtcgtgat cggctcgcgc gacgcgggcc gggccgagga
    10621 ggcggcggga cgtgtgaagg agctcgtgcc ggacgcgaac gtgtcggggg ccgagaactc
    10681 cgcggcggtc gcgtcgtccg agctcgtgat ggtcacggtc ccgttcggcg ggcaggccgc
    10741 catctaccgc tcgatcgccg agagcgtgcg ccccgactcc gtggtggtcg acgggacggt
    10801 ccccgtggcg gcctcggtag gcgcgaaggt cacgcacacc ctcggtgtgt gggagggctc
    10861 ggctgcgcaa caggccgcgg ccttcctccc gaagggcacg cgcacgtgcg cggcgttcca
    10921 ctccttggcg gcggtagcgc tgaacgaccc cgactacgag atggagggtg acgtgctcgt
    10981 gtgcgggccg aagtcggcga agccggtagt gcaggagctg gtggaggcga tccccgggct
    11041 gaggttcgtg gacgcgggcc cgctcgagaa cgcccgcctg gtcgagccga tcaccgcgct
    11101 gctgataggg atcaaccacc gctacgagac cgaccgcagc ggcatccgca tcaccggtat
    11161 ctagcggctg cggagcaggt aacgcgccac gtctttcagg ggggtcagtg gatcgcgcgg
    11221 cggccccatg atcatcaacg tgacccgggt ggagtaatcc acgagcacga cgcgggcagc
    11281 acgttgatcg gcgtaagcga cgttcgtccc gctcagtctc ccgcgcgcca gctccacgtc
    11341 gccgtcgcct gtgtatccgg tcaaaaagtc ctcccacccg ttgggagacc gcgcggctgc
    11401 gggggtccag acgatcccga tcaccagggg ctcggttcca ccggacgcct ccgaggcggc
    11461 tttgacgacg accttctgga tcgaggtctc ggcgagaggg tccttctcca tggttctgac
    11521 cgtcgctcgt ctcacggcgg ccggcgcagg cctgaaggaa tagcggccgt cgcggaagac
    11581 ctcgtcgagc ttggggcgcg ggacacgttc ggtttccgga gccccgacgg agcggacctc
    11641 ggacgcgaac cccccgagcc ctccggcgac gaagagcggc ccccacggtg agcacccccc
    11701 ggccgtcgcc aacacgaaca gccccgccgc caccgccttc cgcccgtcca cggcgcgagc
    11761 gtacctccgc ctaggcttcc tcccatgatg cgaagggcga agatcgtcgc taccgccggc
    11821 cccgccagcc gcagccgcga ggtcctgcgc gagctcgtcg gtgccggcgt cgacgtcctc
    11881 cggctcaacc tcgcgcaccc cagccccgag gcccatgccc aggctgcccg gatcgcccgc
    11941 gaggaggcgt ccgccgtcgg gcgggtcgtc gccgtcctcg ccgacctccc cggccccaag
    12001 atcaggaccg ggccgatcgc cggcggcgag gtcgtgctcg agcccggcgc gacgctctac
    12061 ctcacgacgg agcagatcga aggcgacgcc cgccgcgggt cgacgacgct ggccgccctc
    12121 cccgagatcg tgacgcgcgg cgaccaggtc ttcctcgccg acggcgaggt catcctcgag
    12181 gtcgtctccg ccgacgggac ggacgtccgc acggacgtcg tgcgcggagg gattctgcga
    12241 tcgcgcaagg gcatgcacct cccgcagcac gagggacgga tcgaggcatt caccgagaag
    12301 gaccgcgggt ccttgcgcga cgcggtggcg atgcgcgccg acctggtcgg gctctcgttc
    12361 gtgcgcgacg cagacgacct gcgacgcgtc cgcgcggagc tgcccgacga agacccgccg
    12421 ctgctcgtgg cgaagatcga gacccgctcc gcagtcgaga acctcgcggc gatcgtcgac
    12481 gaggccgacg cggtgatggt cgcccgcggt gacctgggca tccagacgtc gctctccgag
    12541 ctgccgttgc tccagaagcg gatcatcgaa gcctgcaact gcgccgggaa gcccgtgatc
    12601 acggcgaccc agatgctcga gtcgatgacg cgcgcgcccc tgccgacgcg tgcggaggtg
    12661 gccgacgtgg cgaacgcggt gctcgacggt accgacgcgc tgatgttgtc ggaggagacc
    12721 gcggtggggg accggccggt cgaggtcgtg aaggtgatgg acggcatcat cacggccgcc
    12781 gagcgatcgc atcgggaaca tcagtcccct gccagtgacg acctccacga cgacgatccc
    12841 gtctcgtggg ctaccgccca tgccggcgtc caggcggcga cggacctgaa ggctgcagcg
    12901 atcctgtgcc ccacgaggac cggctcgacg gccctgaagg tagcggcgtt ccgaccccct
    12961 atgcccgtcg tcgggatctc gccgcattca caccccaggg cgatgctggc actcgcctgg
    13021 ggggtcgtcc cgctgggctc ggcggattac gcgaccgcgt ctgcggaagg ggccgccggc
    13081 atccccggcg agcggcgcaa gaacgacatc gacgtcggcg tgcgggtcgc gctcgcggcc
    13141 gggacggtcg tggcgggcga cgtcgtcgtg gtcgtcgcgg gaagcgcggc ggcacgcccc
    13201 ggagggaccg acctcgtccg cgtgatgacc tgctagacgg ttagtccttg ggctccaggt
    13261 ccttgaccgc agggccctgg atcaccttcc cgtcgacgtc gaagcgcgag ccgtggcagg
    13321 ggcagtccca ggtcgtttcg gcgttgttcc acgacacgat gcacccgagg tgcgtgcaca
    13381 ccgccgacac cttgtggagc tccccggcat cgtcgcggta ggccgcgacc tgacccggcg
    13441 cggtcccgac gacggcggct tcacccggag cgaggtcgcc cggcacgccg gactgcggat
    13501 ggctcagccg gtcgcgcagc cagtgccccg cgaccttcac gttctccttc gcgaacttcg
    13561 gtgccgacgc cttgagcgtc gcgcggtgcg ggtcgtacag agacgcccac tcgttcggtg
    13621 tctcggtgat caggtcggcg aggatcatcc cggcaagcgt cccgttcgtc atgccccact
    13681 tccggaacgc ggtggctgtg taagcgcggc gcgacaccgg cgtgagccgc ccgatgtagg
    13741 gcagcgtgtc gacggacgtg ccgtcctgcg tcgaccaccg gtagcgcact tcggtcatcc
    13801 cgagcttctc gcgcgaccac cgctcgagct cctcgtaccg cgcctcggtg tcgtaatgct
    13861 gccccacggt gtgacctgca ccgcccgtca gcacgagggt ctcgtcgccg acgcgcaccg
    13921 gccggaccga ccacgccggc tcggtgctca gatacatccc ctccggcacc gtgccggaga
    13981 cgggtccgac catcgcgtac gacctcgacg ggtggacgcg cgggaacatc atcgagcggt
    14041 agaggaacct gtagtgggac gcgacgacga cgtggtccgc gctcacattg ccgccgctcg
    14101 tctcgacgag gcacgggtcc ccg
//
LOCUS       DASQDO010000172         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_935382, whole genome shotgun sequence.
ACCESSION   DASQDO010000172 DASQDO010000000
VERSION     DASQDO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935382"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VEV43_05085"
     CDS             <1..1047
                     /locus_tag="VEV43_05085"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HYP22926.1"
                     /translation="DVGASIGVSMFPDHARGVDALLQRADVAMYAAKETQTGYEFYNP
                     DRDQNSLRRLALGSELSNAIQNKEVVLYYQPKADMRTGKIIGVEALARWQHAYRGVVG
                     PDEFIPLAEQTQLIRPLTMNLLDEAIRQISVWDKRGFKLKVAVNLSSRSLLDLQLPDQ
                     VTRLLQKWGVEATSLELEITESTIMADARRALAVLSTLNAMGVGLAIDDFGTGFSSLS
                     YLKRLPVKEMKMDKSFVMKMASDENDAAIVRSTIELAHNLGLRVVAEGVENEGIWKQL
                     STLGCDLAQGYYLTRPVPANELEEWLQDYNAARFPAATRRQPPPPDEWERQLLGDHPA
                     GYFAGPSLGSASSE"
     gene            complement(993..1799)
                     /locus_tag="VEV43_05090"
     CDS             complement(993..1799)
                     /locus_tag="VEV43_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22927.1"
                     /translation="MTGTRRKTRLAALLLTLVVAAACSSPQGTEQGDGSRLGKEKPRG
                     TAAAGKGDKDGGAKPGGKKTDGGSQVPPSAVPGGVAMPSPGVAEKPGGFSGASRTKTT
                     TTSNGVDPRTASVVVTEPDPDADKSGVAPEFADVLSTRVEGDGPNLRVTITFRGQLPQ
                     KMPDDKTYMVTGFGLTSPKGQSQGYAFGASADVKGWTAYGGGKDKKDFPGEFSISGDT
                     AVFLIPWSAIQGPRPFEWYAQASCFRSLAGTTHYSLDALPNEGPAKYPAG"
     gene            complement(1796..1954)
                     /locus_tag="VEV43_05095"
     CDS             complement(1796..1954)
                     /locus_tag="VEV43_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22928.1"
                     /translation="MTPTPLPSPSASFVFDLPQAAPGEAIGAVVAIVLALAAGILGYR
                     IIRGGRGL"
     gene            complement(1951..2568)
                     /locus_tag="VEV43_05100"
     CDS             complement(1951..2568)
                     /locus_tag="VEV43_05100"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYP22929.1"
                     /translation="MPTEFEQSLRAALAAHSPARVRVSDARAAAVLIPVVAEEEPSVL
                     FTLRTETVRSHKGQISFPGGSIDPTDRSARAAALREAQEEIGLDPSLVEIVGELDTTP
                     TFVSGFVISPFVGLLSETPPLTPNPDEVAEVLQVPLADLNDEIRAEPGFAHAGRTYPT
                     EAWIWRDYVIWGVTARLIRLFLGVLAEGGLVAAPSETTSWSGFSA"
     gene            2621..>3014
                     /locus_tag="VEV43_05105"
     CDS             2621..>3014
                     /locus_tag="VEV43_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYP22930.1"
                     /translation="MGEFVNLEVAASGVATLRLDRPKMNALNQQVAREIGEAVDAAAE
                     NADVRALVVWGGERIFAAGADIKEMAELDAVSMYRYIGRFQDVFTRLEQLPVVTIAAV
                     NGYALGGGCELALACDLRICAEDARLGQP"
BASE COUNT          455 a         1047 c         1054 g          458 t
ORIGIN      
        1 gacgtgggag cgagcatcgg agtttcgatg ttcccggacc acgcgcgagg cgtcgacgcg
       61 ttgctgcagc gggccgacgt cgccatgtac gcggcgaagg agactcagac cggctacgag
      121 ttctacaacc ccgaccgcga ccagaacagc ctccggcggc tcgcgctcgg cagcgagctg
      181 agcaacgcga tccagaacaa ggaggtcgtc ctctactacc agccgaaggc cgacatgcgc
      241 acgggcaaga tcatcggcgt cgaggcgctg gcccggtggc agcacgcgta ccgcggtgtg
      301 gtcggtccgg acgagttcat accgctggcc gagcagacgc agctgatccg cccgctgacg
      361 atgaacctgc tcgacgaggc gatccgtcag atctcggtgt gggacaagcg cgggttcaag
      421 ctgaaggtcg cggtgaacct gtcctcgcgc agcctgctgg acctgcagct cccggaccag
      481 gtcacgcgcc tgctccagaa gtggggcgtc gaggcgacct ccctcgagct cgagatcacg
      541 gagagcacga tcatggcgga cgctcggcgc gccctcgcgg tgctgtcgac cctgaacgcg
      601 atgggcgtcg gcctcgcgat cgacgacttc ggcaccgggt tctcgtcgct gtcgtacctc
      661 aagcgtctcc cggtcaagga gatgaagatg gacaagtcct tcgtcatgaa gatggcgtcc
      721 gacgagaacg acgcggcgat cgtccgttcg acgatcgagc tggcgcacaa cctcgggctg
      781 cgcgtcgtcg ccgagggggt cgagaacgag gggatctgga agcagctctc gacgctcggc
      841 tgcgacctcg cgcagggcta ctacctcacg cggccggtcc cggcgaacga gctggaggag
      901 tggctacagg actacaacgc agcgcgcttc cccgccgcga cgcggcgcca gccgccgcct
      961 cccgacgagt gggagcgcca gctcctcgga gatcaccccg ccgggtactt cgccggcccc
     1021 tcgttgggga gcgcgtccag cgagtagtgc gtggtccctg cgagggacct gaaacacgag
     1081 gcctgcgcgt accactcgaa cgggcgcggc ccctgtatcg cggaccacgg gatcaggaac
     1141 acggccgtgt cgcccgagat cgagaactct cccgggaagt ccttcttgtc cttgccgccg
     1201 ccgtaggccg tccagccctt cacgtcggcg gacgcgccga acgcgtagcc ctgcgactgt
     1261 cccttcgggc tcgtgaggcc gaaccccgtg accatgtacg tcttgtcgtc gggcatcttc
     1321 tgcgggagct ggccgcggaa cgtgatcgtc acccggaggt tcgggccgtc cccctcgacc
     1381 cgcgtcgaca agacgtcggc gaactccggc gcgacgccgc tcttgtccgc gtcggggtcg
     1441 ggctcggtca cgacgaccga cgccgtccgc gggtcgacgc cgttcgaggt cgtcgtggtc
     1501 ttggtcctgg atgctccgct gaaccctccg ggcttctcgg ccacgccggg ggatggcatc
     1561 gcgacgccgc cggggacggc cgacggcggg acctgcgagc cgccgtccgt cttcttccct
     1621 ccgggcttcg cgccgccgtc cttgtcgcct tttccggcgg cggcggtgcc gcgcggcttc
     1681 tccttgccga gccgcgagcc gtcgccttgc tcggtgccct gcgggctgga gcaggccgcg
     1741 gccacgacca gcgtcagcaa cagcgccgcc agcctcgtct tcctccgggt ccccgtcaca
     1801 gtcctcgacc ccctctgatg atcctgtagc ccagtatccc cgcggccagc gccaacacga
     1861 tggcgacgac ggcgccgatc gcctcgccgg gagccgcctg gggcaggtcg aacacgaacg
     1921 acgccgacgg ggacggcagg ggtgtcgggg tcatgccgag aacccgctcc acgacgtggt
     1981 ctccgagggg gccgctacga gaccgccttc cgcgagcacc cccaggaaga gccggatgag
     2041 ccgggcggtc acgccccaga tgacgtagtc gcgccagatc cacgcctcgg tgggataggt
     2101 gcggccggcg tgtgcgaacc cgggctcggc ccggatctcg tcgttcaggt cggccagcgg
     2161 gacctgcaac acctcggcga cctcgtccgg gttcggcgtc agcggaggcg tctcggacag
     2221 cagccccacg aacgggctga tcacgaagcc gctgacgaac gtcggcgtcg tgtcgagctc
     2281 gccgacgatc tcgacgagcg aagggtcgag cccgatctcc tcctgtgcct ctctcagcgc
     2341 cgcggcccgc gccgatcggt cggtgggatc gatcgatccg ccggggaacg agatctgtcc
     2401 cttgtggctg cgcacggtct cggtcctgag cgtgaagagc accgacggct cctcctccgc
     2461 gacgaccggt atcaggaccg ccgccgctcg tgcgtcggag acgcgtaccc gcgcggggct
     2521 gtgcgccgcc agcgcggcgc gtaaggactg ctcgaactcg gtcggcacgc ggagatcgta
     2581 gccttgcgat cctgcaatcg atcctggcgc gggaggcatc gtgggagagt tcgtgaacct
     2641 cgaggtagcg gcctctggcg tggcgacgct gaggcttgac cggccgaaga tgaacgcgct
     2701 caatcagcag gtcgcgcgcg agatcggcga ggcggtcgac gccgccgcgg agaacgcgga
     2761 cgtgcgcgcc ctggtggtct ggggcggcga gcggatcttc gccgccggtg ccgacatcaa
     2821 ggagatggcc gagctcgacg cggtctcgat gtaccggtac atcggccgct tccaggacgt
     2881 cttcacgcgt ctcgagcagc tgccggtggt gacgatcgcc gcggtcaacg gctacgcgct
     2941 cggcggtgga tgcgagctcg cgctggcgtg cgacctccgc atctgcgccg aggacgcgcg
     3001 gctgggccag ccgg
//
LOCUS       DASQDO010000173         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_507211, whole genome shotgun sequence.
ACCESSION   DASQDO010000173 DASQDO010000000
VERSION     DASQDO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507211"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2105
                     /locus_tag="VEV43_05110"
     CDS             <1..2105
                     /locus_tag="VEV43_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HYP22931.1"
                     /translation="SGYQGGKGEPAAGPGEGTPTTSLVLDQFGRNLTQAARESKLDPV
                     IGRAKEIERVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQNIVKGEVPETIAGKQLY
                     TLDLGALVAGSRYRGDFEERLKKVLKEINTRGDIILFIDELHTLVGAGAAEGAIDAAS
                     ILKPMLARGELQTIGATTLDEYRKHLEKVAALERRFQPIKVPEPTVAHTIDILKGLRD
                     RYEAHHRVHITDQGLVAAANLADRYISDRFLPDKAIDLIDEAGSRLRIRRMSAPPDYR
                     ELEERIAEVRRAKEDAIEAQDFERAAAFRDDEKRLIAERAEKETTWRAAEGEAFAEVN
                     EEEIAEVLSSWTGIPVMSLTEEETAKLLRMEEELHQRIVGQHDAIAAVSRSIRRTRAG
                     LKDPKRPAGSFIFLGPSGVGKTETAKTLAEFLFGDEDALIQLDMSEYMEKHTVSRLVG
                     SPPGYVGYEEGGQLTEAVRRRPYSVVLLDEIEKAHPDVFNTLLQILEDGRLTDAQGKT
                     VDFKNTVIIMTSNLGTQNLRKPAMGFGQASEELTYEKMKERVNEELKKSFRPEFLNRI
                     DEVIVFHELSQDEVKSIVDLMIRRVKEQLESQDVGIELTEAAKVLLSKRGYDPTRGAR
                     PLRRSIQRLVEDPISEKILWKEYEAGDTIIVDVGPGEDGEDSIVFKKAERTPDHPPVE
                     LAGAGLGDGEPDAALPDA"
     gene            2277..>2963
                     /locus_tag="VEV43_05115"
     CDS             2277..>2963
                     /locus_tag="VEV43_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22932.1"
                     /translation="MIRGNRIFAGALLALLVVGALSGAAALAEERVDEAVQETEAPLD
                     VVATLPSEDVGDQPRTSPAEEPAAEEPGDQVFEETWWKRRLLGKSERRPEPEPEPEPE
                     PLPEEPEPEPEPAAEVAEVAEVAYEEPETVAAYEDPEVAEVTYDEPAAYVEDEELVYA
                     DDEALVYADDDDELLEDEPLPDLTDVGDDAPASSLSANVPGPRRARLEGERRVKRRRE
                     LTRGAIAAAVA"
BASE COUNT          534 a         1025 c         1032 g          372 t
ORIGIN      
        1 tctccggcta ccagggcggc aagggcgagc ccgccgcggg cccgggcgag ggcacgccga
       61 cgaccagcct ggtgctcgac cagttcggca ggaacctcac ccaggcggcc cgcgagtcca
      121 agctcgaccc ggtcatcggc cgggcgaagg agatcgagcg cgtgatgcag gtcctgtcgc
      181 gccgcaccaa gaacaacccc gtcctcatcg gcgagcccgg cgtcggcaag accgcgatcg
      241 tcgagggcct cgcgcagaac atcgtcaagg gcgaggtgcc cgagaccatc gcgggcaagc
      301 agctgtacac gctcgacctc ggcgctctcg tcgccggctc ccgctaccgc ggcgacttcg
      361 aggagcggct gaagaaggtc ctcaaggaga tcaatacccg cggcgacatc atcctgttca
      421 tcgacgagct ccacacgctc gtcggcgcgg gtgcggccga gggcgcgatc gacgcggcga
      481 gcatcttgaa gccgatgctg gcccgcggcg agctccagac catcggcgcc acgacgctcg
      541 acgagtaccg caagcacctc gagaaagtcg ccgccctcga gcgccggttc cagccgatca
      601 aggtgcccga gccgaccgtc gcccacacga tcgacatcct caaggggctg cgcgaccgct
      661 acgaggcgca ccaccgggtc cacatcacgg accagggcct cgtcgccgcg gcgaacctcg
      721 ccgaccgcta catctccgac cgcttcctgc ccgacaaggc gatcgacctg atcgacgagg
      781 ccgggtcgcg gctgcgcatc cgcaggatga gcgccccgcc cgactaccgc gagctcgagg
      841 agcgcatcgc cgaggtccgc cgcgccaagg aggacgcgat cgaggcgcag gacttcgagc
      901 gtgccgccgc cttccgcgac gacgagaagc ggctgatcgc cgagcgcgcc gagaaggaga
      961 ccacctggcg cgctgccgag ggcgaagcct tcgccgaggt caacgaggag gagatcgccg
     1021 aggtcctgtc gtcgtggacc ggcatccccg tgatgtcgct gaccgaggaa gagaccgcga
     1081 agctgctccg gatggaagag gagctccacc agcggatcgt cggccagcac gacgcgatcg
     1141 ccgcggtgtc ccgctcgatc cgccgcacca gggcgggcct caaggacccc aagcgtcccg
     1201 ccggttcgtt catcttcctc ggcccctcgg gcgtcgggaa gaccgagaca gcgaagacgc
     1261 tcgccgagtt cctgttcggc gacgaggacg cgctgatcca gctcgacatg tccgagtaca
     1321 tggagaagca cacggtgtcc cgcctcgtcg gctcgcctcc gggctacgtc ggctacgagg
     1381 agggcggcca gctcaccgag gccgtgcggc gcaggccgta ctcggtcgtg ctgctcgacg
     1441 agatcgagaa ggcccacccg gacgtcttca acacgctgct gcagatcctc gaggacggcc
     1501 gtctgacgga tgcccagggc aagacggtgg acttcaagaa caccgtgatc atcatgacgt
     1561 cgaacctcgg tacgcagaac ctgcgcaagc ctgcgatggg cttcggccag gcgtcggagg
     1621 agctgaccta cgagaagatg aaggagcggg tcaacgagga gctgaagaag agcttccgtc
     1681 ccgagttcct gaaccgcatc gacgaggtca tcgtgttcca cgagctttcg caggacgagg
     1741 tcaagtcgat cgtggacctg atgatccggc gggtgaagga gcagctcgag agccaggacg
     1801 tcggcatcga gctcaccgag gccgccaagg tcctgctctc gaagcgggga tacgacccga
     1861 cgcggggggc ccggccgctg cgccgctcga tccagcggct ggtcgaggac ccgatctccg
     1921 agaagatcct ctggaaggag tacgaggccg gcgacacgat catcgtcgac gtcgggccgg
     1981 gcgaggacgg cgaggactcg atcgtcttca agaaggccga gcggacgccg gaccacccgc
     2041 ccgtcgagct ggccggcgcc ggtctcggcg acggcgagcc ggacgcagcg ctgccggacg
     2101 cctagctcct cgaagtagtc caaggcacag ggccccgcgg gccgtcccgc ccggggggtc
     2161 ttgtcgcgtc cgggccggga cttccggaac ttgtccccga tgcgtccagt gaggggtacc
     2221 gggtgccgac aactagtcat acggcgacca tcgtcgccag actgggggca cacgacatga
     2281 tcaggggaaa ccggatcttc gcgggcgcgt tgctcgcgct gttggtcgta ggggcgctct
     2341 ccggcgcggc cgcacttgcg gaggagcgcg tggacgaggc ggtccaggag accgaggccc
     2401 ccttggatgt cgtcgccacg ctgccttcgg aggacgtcgg cgaccagcca cggacctccc
     2461 ctgcggagga gccggctgcg gaggagccgg gcgaccaggt cttcgaggag acgtggtgga
     2521 agcgccgtct gctgggcaag tccgagcgtc gccccgagcc tgagcccgag cccgaacccg
     2581 aacccctccc cgaggagccg gaacctgaac ccgagcccgc cgccgaagtc gccgaggtcg
     2641 cggaggtcgc ctacgaggag ccggagacag tcgccgccta cgaggacccc gaggtcgcgg
     2701 aggtcacgta cgacgagccg gcggcctacg tcgaggacga ggagctcgtc tacgcggacg
     2761 atgaggcgct cgtctacgcg gacgacgacg acgagctgct cgaggacgag ccgctgcccg
     2821 acctcaccga cgtaggtgac gacgcccccg cctcctctct cagcgcaaac gtccccggcc
     2881 cccggagagc gcgcctcgaa ggcgaacgcc gggtcaagcg ccgcagggag ctgacacgcg
     2941 gggcgatagc cgcggcagtg gcc
//
LOCUS       DASQDO010000174         9206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_630167, whole genome shotgun sequence.
ACCESSION   DASQDO010000174 DASQDO010000000
VERSION     DASQDO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630167"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..929)
                     /locus_tag="VEV43_05120"
     CDS             complement(156..929)
                     /locus_tag="VEV43_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYP22933.1"
                     /translation="MTVRAVTGAPVAEVVLDRPDARNALSIEMCRGIVAALDEVRATS
                     ARCVLLRGEGKVFCSGADFAAVSGPGGLEFLPAFEEMLTAVESFPLPVVAMIQGAALG
                     GGLQLATVCDFRIAADDAKVGIPAARIGIVVNFENVERLVRLAGVAAAKEILMTASTM
                     TGGEAEARGLVTKAVPALDLEATARAFAQEIADLSPQSVQGAKRSIGLVERALVEARR
                     THPDEVAEIDGLVAAAYGSTDLQEGLAAMAERRPPRWEG"
     gene            complement(926..1147)
                     /locus_tag="VEV43_05125"
     CDS             complement(926..1147)
                     /locus_tag="VEV43_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HYP22934.1"
                     /translation="MSEKVLAEMVANVWKVVASAGDAIEEGGTICILESMKMEIPVEA
                     PVSGTLTEIRVDEGGVVQEGDVIAVIEPR"
     gene            complement(1144..2031)
                     /locus_tag="VEV43_05130"
     CDS             complement(1144..2031)
                     /locus_tag="VEV43_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007258381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HYP22935.1"
                     /translation="MDSCIVTCALSGVAANRDQCPAIPYTPEEYAAEARRARDAGAAV
                     VHIHARYPDGRPSYRIEDYRAITQAILDAAPDLVVNFSTGAVGIRIEERVQQVTALRP
                     ELAALNMGSMNYAKYSRKRKSFVFEFVFANPFADIVYLLEQMRSAGVKPECECFDTGH
                     VASLEPLIDMGLLEPPFQLSLIVGVLGGAPADARTLAHLATLVPQPSTWEVVAISRAQ
                     WTLVSAAASLGGNVRVGLEDNFYLPSGEMASSNGDLVEAAVRLVELSGREVADPATAR
                     TMLALPDPPDRSALMARAS"
     gene            complement(2024..3007)
                     /locus_tag="VEV43_05135"
     CDS             complement(2024..3007)
                     /locus_tag="VEV43_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HYP22936.1"
                     /translation="MKPDPLNPWRRLVWPGLLLVGMAVYGVAGYMLLGWSFVDAIYMV
                     VLILSSVGFREVRPLETGGQVFTISLMLLGVTLLLITVALLAQAVAEGGLGARGRRRR
                     MQRAVERMSGHFIVCGYGRVGRTIARELEGEGAPFVVVDRNDELEEELMRDGVVNLIG
                     DATSDDTLKAAGIDRAKALVCALDSDANNIFVTIVARALSADVYIVARASQETAADRM
                     YKAGADRVVSPYVSSGRHMALLALRPRVVDYMDVTTDEGPGLRLEEVVVEARSALEGR
                     LLSEIAGDAVPLVVKRADGELIHTPDSTMRLGDGDVLVLLSDLPAQDSARG"
     gene            complement(3004..4602)
                     /locus_tag="VEV43_05140"
     CDS             complement(3004..4602)
                     /locus_tag="VEV43_05140"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HYP22937.1"
                     /translation="MSDTRSSITYSYDGPEMRDHVAALAERKAKHLGMGGPDRVQRQH
                     DAGKLTVRERLDRLFDGGSFQEHGLLAHHQSTSPAMQGKFTPADGVVTGVGKVDGRPV
                     AVIAYDFTVMAGSMGMVTEMKATRMRELALRERIPLVWLIDSAGARIQEATGSMFART
                     GDLFREQVVMSGVVPQVAAMMGPGAAGTAYIPGLADFVPMVKGTSNMALAGPHLVKAA
                     VGEDVTAEEMGGSQVHCKVSGVADLEVADDEACLTAVRKYLSYFPSSNLERPPASDTE
                     DPAGRRTEELYDKVPANPRRAYDVHDVIRALTDDGDFFPMKPDWARNVVTGFARFGGR
                     PGGIVANPPKFLGGALDVNAADKAARFVWLCDAFSIPLVFLMDCPGFLVGSAVEKQGI
                     IRHGAKMLFAVAEATVPKITVVMRKGYGAGYYVMNGRAYEPDLIVAWPTAEISVMGPE
                     GAVNIIFRKQIEALPEEERDEARNGMVAMIREQISPYVAAGWSFVDDVIDPADTRATI
                     CAGLEAAQDKKVERPWRKHGVLPV"
     gene            complement(4681..5460)
                     /locus_tag="VEV43_05145"
     CDS             complement(4681..5460)
                     /locus_tag="VEV43_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYP22938.1"
                     /translation="MTAGPVAYAVAGGVATLTIDRPERRNAINPEVTRALRAALARAE
                     ADDSVRVAVLTGAGDKAFCAGGDLGGMTADSKVGQHRERAEVGELFAAMRASRLPIVA
                     RVNGHALAGGFGQMLACDLVVAVDDAEMGMPEIDVGLWPFMVTAIVQRDVPRKLALEL
                     MLTGRGIPASEGARWGFVNRVVSRNDLDAAVAELTGSIASKSPLIAALGKSSFYRAED
                     MSFEAQLDYLAGMLTVCLESEDTVEGVTAFLEKRAPEWKGR"
     gene            complement(5457..6089)
                     /locus_tag="VEV43_05150"
     CDS             complement(5457..6089)
                     /locus_tag="VEV43_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22939.1"
                     /translation="MARTNLHELANRLLETLASSAEGDTFEGKLRAVAAEAGLNSVRS
                     AEVIKLLEELGRIEVVQRGRRGRNTIIKLASTDPVKLEDAEAMLPSRAAKRTTRLNYD
                     DLGKAVVARLLELARDDGLRAAQVEAFAAESAAHRKRVEELEEQLEEAKLRETDVRVR
                     LKAAEEALERAEENLRKAFGSSPRPVDGGATSIEDEDARAVLDILRSGRG"
     gene            complement(6183..6380)
                     /locus_tag="VEV43_05155"
     CDS             complement(6183..6380)
                     /locus_tag="VEV43_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP22940.1"
                     /translation="MLELIDREDERYLKTAEAARILRVSPKTVSRWAKDGKLPHMVTL
                     GGHRRFPASRIEAIARGLEAI"
     gene            complement(6466..6627)
                     /locus_tag="VEV43_05160"
     CDS             complement(6466..6627)
                     /locus_tag="VEV43_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22941.1"
                     /translation="MARRYRFRYYPGVRDALSTAPPEARHAFGETNLLLFQVMADYPL
                     IRLIDVIIA"
     gene            complement(6642..6944)
                     /locus_tag="VEV43_05165"
     CDS             complement(6642..6944)
                     /locus_tag="VEV43_05165"
                     /inference="COORDINATES: protein motif:HMM:NF039700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6247 family protein"
                     /protein_id="HYP22942.1"
                     /translation="MTSIPDRIVRPEELGLPSEAREVFDRLTTEGARAFSRDIVQALL
                     EAQARGNLRPVRDVVDAWYRTLQFVQQPHHDEAVAWAGRDDGPDLDREDLERQLGL"
     gene            complement(7072..7305)
                     /gene="rsrA"
                     /locus_tag="VEV43_05170"
     CDS             complement(7072..7305)
                     /gene="rsrA"
                     /locus_tag="VEV43_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HYP22943.1"
                     /translation="MKEICRETLERAYLLIDGEPIAEAESLSIRAHLEECSPCYERYG
                     LEVEVKSLISRLRGAHPCPEALKHRIAGLIEEA"
     gene            complement(7324..7938)
                     /locus_tag="VEV43_05175"
     CDS             complement(7324..7938)
                     /locus_tag="VEV43_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP22944.1"
                     /translation="MAEAKRVELSPADKARFTRDTLPLLDSLYASALRMTRNPADAED
                     LVQETMLRAYRSFDRFEEGTNLKAWLFRIMTNAYINTYRKKQREPKKVSADEVEDFDL
                     YQELKNHDDQFSQTPERIVLDSLVDTDIIEAIDDLPEQFRMAVVLSDVEGFSYAEMAE
                     IMDVPLGTVMSRLHRGRKALQKRLWDLARDRGIVRSSSDGSRGA"
     gene            complement(7931..9106)
                     /locus_tag="VEV43_05180"
     CDS             complement(7931..9106)
                     /locus_tag="VEV43_05180"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HYP22945.1"
                     /translation="MAGNLTVGVIFGGRSVEHDVSIVTAHQVMAAARDRYDVVPIYVA
                     RDGRWFSSPALDDLDVYKKGRHEEAGEEVALALDGGGLQAPGGRLKGPRRIPLDVVIP
                     AIHGTYGEDGTLQGLLEMAGLPYAGSDVLASAIGMNKVEMKTVFAAAGLPVVPHEVVA
                     VAELDADLDAVVDRVEGSIGYPAFVKPSRLGSSVGIGRAPDRAALADALDVARRYDDR
                     ILVEKAMEGCMEVNCSVLGGAGLPPRASVCEQPVSWREFLSFEDKYMRGGKGSGSKGE
                     GMASQDRRIPAPIPDELTKQVQDNALAAFAAVGAAGVARIDSFVNRETGDTWVMEINT
                     VPGSFAFYLWEASGVTFRDLVGSLVDSALARHAAKGKLMFSFESGLLAGFGGGKAGG"
BASE COUNT         1350 a         3325 c         3094 g         1437 t
ORIGIN      
        1 caggcagggc cgcggtcgga cgagccgtcg cccgcggagc acgcgacggt caggatcccc
       61 cacagagcga cgccggcgac gagcgccggc gtgagtcgtt tacccacaca cccatcatcg
      121 cgccgccggg aatcccccac ccgggggagg cccggctagc cctcccagcg gggagggcgg
      181 cgctcggcca tcgcggccag gccctcttgc aggtcggtgc tcccgtaggc cgctgcgacc
      241 aggccgtcga tctcggccac ctcgtccggg tgcgtccgcc gggcttcgac gagcgcccgc
      301 tcgaccaggc cgatggagcg cttcgctccc tggaccgact gcggcgacag gtccgcgatc
      361 tcttgcgcga aggcgcgggc agtcgcttcc aggtcgaggg ccgggacggc cttcgtcacc
      421 aggccgcgcg cctcggcttc gccccccgtc atcgtgctcg ccgtcatcag gatctccttc
      481 gcggcggcga cgccggcaag gcgcacgagc cgctcgacgt tctcgaagtt cacgacgatg
      541 cctatgcggg ccgccgggat gccgaccttc gcgtcgtccg ccgcgatcct gaagtcgcac
      601 accgtcgcga gctggagccc gccgccgagg gccgcgccct ggatcatcgc cacgaccggc
      661 agtgggaacg actcgacggc cgtcagcatc tcctcgaacg ccgggaggaa ctcgagcccg
      721 cccggccccg acaccgccgc gaagtccgcc cccgagcaga agactttgcc ctccccgcgg
      781 agcaggacgc agcgcgcgct cgtcgcgcgg acctcgtcca gggccgcgac gatcccgcgg
      841 cacatctcga tcgatagagc gttgcgcgcg tccggacgat cgagcacgac ctcggccacc
      901 ggcgcaccgg tcacggcacg gaccgtcacc gcggctcgat caccgcgatc acgtcgccct
      961 cctggaccac gccgccttcg tccactctga tctcggtcag cgtgccggag acgggggcct
     1021 cgacggggat ctccatcttc atcgactcga ggatgcagat cgtcccgcct tcctcgatcg
     1081 cgtcacccgc cgacgcgacg accttccaaa cgttcgcgac catctccgca aggactttct
     1141 cgctcacgac gccctcgcca tcagcgcact gcggtccggt gggtccggca gagccagcat
     1201 cgtccgcgca gtcgccgggt cggcgacctc gcgccccgag agctctacga gccgcaccgc
     1261 ggcctcgacg agatcgccgt tcgacgacgc catctcgccc gacggcaggt agaagttgtc
     1321 ctcgaggccg actctcacgt tgccgcccag cgacgcggcc gccgagacga gcgtccactg
     1381 ggcccggctg atcgcgacga cctcccacgt gctcggctgc gggaccagcg tcgccaggtg
     1441 cgcgagcgtg cgagcgtcag ccggcgcgcc gccgaggacg ccgacgatca gcgacagctg
     1501 gaacggaggc tcgagcagtc ccatgtcgat caacggctcg aggctagcga cgtggccggt
     1561 gtcgaagcat tcgcactccg gcttgacgcc ggccgaccgc atctgctcga gcaggtagac
     1621 gatgtcggcg aacgggttgg cgaacacgaa ctcgaagacg aacgacttgc gcttgcgcga
     1681 gtacttcgcg tagttcatcg agcccatgtt cagcgccgcg agctcgggcc gcagcgcggt
     1741 gacctgctgg acgcgctcct cgatgcggat gccgacggcg ccggtcgaga agttcacgac
     1801 gaggtcgggg gccgcgtcca ggatcgcctg cgtgatcgcg cggtagtcct cgatgcggta
     1861 gctcggacgt ccgtccggat agcgggcgtg gatgtggacg accgcggccc ccgcatcgcg
     1921 cgcccgcctc gcctccgccg cgtactcctc cggcgtgtag gggatcgccg ggcactggtc
     1981 ccggttcgcc gcgaccccgc tcaacgcgca ggtcacgatg cagctatcca cgcgccgagt
     2041 cctgggccgg gaggtcggac aagagcacca gcacgtctcc gtcgccgagc ctcatcgtcg
     2101 agtcgggcgt gtggatcagc tctccgtcgg cgcgcttgac gaccagcggg accgcgtcac
     2161 ccgcgatctc cgacagcagg cgcccttcga gggccgagcg ggcctcgacg acgacctcct
     2221 cgagccgaag gcccggtccc tcgtccgtcg tcacgtccat gtagtcgacg acccgcgggc
     2281 ggagggcgag caaggccatg tgccgcccgc tgctcacgta cggggacacg acccggtcgg
     2341 caccggcctt gtacatgcgg tcggccgcgg tctcctggga cgcgcgcgcc acgatgtaga
     2401 cgtccgcgct gagagcgcgg gcgacgatcg tcacgaagat gttgttggca tcggagtcga
     2461 gcgcgcagac cagagccttc gcccggtcga tgcccgccgc cttcaacgtg tcgtccgagg
     2521 tcgcgtcgcc gatcaggttc acgaccccgt cgcgcatcag ctcctcttcg agctcgtcgt
     2581 tgcggtcgac gacgacgaac ggagcgccct ccccttcgag ctcgcgcgcg atcgtgcgcc
     2641 cgacgcggcc gtagccgcag acgatgaagt gcccgctcat ccgttcgacc gcgcgctgca
     2701 tgcgccttct cctaccacgg gcgccgaggc ccccttccgc cacggcttgg gcgaggagcg
     2761 ccaccgtgat gagcagcaag gtgacgccga ggagcatcag gctgatcgtg aagacctggc
     2821 cccccgtctc gagcgggcgc acctcgcgga acccgacgct gctcaggatc agcacgacca
     2881 tgtagatcgc gtcgacgaac gaccatccca gcagcatgta gccggcgacg ccgtagacgg
     2941 ccatcccgac gaggaggagg cccggccaca cgagccggcg ccacgggttc agcgggtccg
     3001 gcttcacacc ggcagcaccc cgtgcttgcg ccagggccgc tcgaccttct tgtcctgcgc
     3061 cgcctccagc cccgcgcaga tcgtcgcgcg cgtgtcggcg gggtcgatga cgtcgtcgac
     3121 gaacgaccag cccgcggcga cgtacggcga gatctgctcg cggatcatcg cgaccatgcc
     3181 gttgcgcgcc tcgtcacgct cctcctccgg caacgcttcg atctgcttgc ggaagatgat
     3241 gttcaccgcg ccctccggcc ccatcaccga gatctccgcc gtcggccatg cgacgatcag
     3301 gtcgggctcg taggcgcggc cgttcatcac gtagtagccc gcgccgtagc ccttgcgcat
     3361 cacgacggtg atcttcggga ccgtggcctc cgcgaccgcg aacagcatct ttgctccgtg
     3421 gcggatgatg ccctgcttct cgaccgcgct cccgacgagg aatccggggc agtccatcag
     3481 gaacacgagc gggatcgaga acgcgtcgca cagccacacg aagcgcgcgg ccttgtcggc
     3541 cgcgttgacg tcgagggccc cgccgaggaa cttgggcggg ttggcgacga tccccccggg
     3601 gcggccgccg aagcgggcga acccggtgac gacgttgcgg gcccagtcgg gcttcatcgg
     3661 gaagaagtcg ccgtcgtcgg tgagggcccg gatgacgtcg tggacgtcgt aggcgcggcg
     3721 agggttcgcc gggaccttgt cgtagagctc ctcggttcgg cggccggcgg ggtcctccgt
     3781 gtccgacgcg ggcggtcgct ctaggttcga cgaagggaag tacgacaggt atttgcggac
     3841 cgcggttagg caggcctcgt cgtcggccac ctcgaggtcg gcgacgccgg agaccttgca
     3901 gtgcacctgc gacccgccca tctcctcggc cgtcacgtcc tcgccgaccg cggccttgac
     3961 gaggtggggg ccggcgagcg ccatgttcga cgtccccttg accatcggga cgaagtccgc
     4021 gaggcccggg atgtaggcgg tccccgctgc gcccggcccc atcatcgcgg cgacctgcgg
     4081 gaccacgccg ctcatcacga cctgctcgcg gaagaggtcg ccggtgcgcg cgaacatcga
     4141 gccggtcgcc tcctggatgc gcgcgcccgc ggagtcgatc agccacacga gcgggatgcg
     4201 ctcgcgcagc gctagctcgc gcatccgcgt cgccttcatc tcggtgacca tccccatcga
     4261 gccggccatc acggtgaagt cgtaagcgat caccgcgacc ggccgtccgt cgaccttgcc
     4321 gacgccggtc acgacgccgt ccgcgggcgt gaacttgccc tgcatcgcgg gtgacgtgga
     4381 ctggtggtgg gcgagcaggc cgtgctcctg gaacgagcct ccgtcgaaga gccggtcgag
     4441 ccgctcgcgc acggtcagct tgccggcgtc gtgctggcgc tggacccggt cgggccctcc
     4501 catgccgagg tgcttcgcct tgcgctcggc cagggcggcg acgtggtcgc gcatctcggg
     4561 gccgtcgtac gagtacgtga tggagctccg cgtgtcggac acggttgcaa cacctgcctt
     4621 tgggcccttc gccggcgggt ctcgccgaag tcttcccggg ctcgcgggag ggagcctagt
     4681 tcagcggccc ttccattcgg gggcgcgctt ctccaggaag gccgtcacgc cctccacggt
     4741 gtcctcggat tcgaggcaca ccgtgagcat ccccgccagg tagtcgagct gggcctcgaa
     4801 cgacatgtcc tcggcccggt agaacgacga cttgccgagg gccgcgatca acgggctctt
     4861 cgacgcgatc gatccggtca gctccgcgac cgcggcgtcc aggtcgttcc tcgagacgac
     4921 acggttcacg aacccccagc gagcgccctc ggaggccggg atgccgcggc cggtgagcat
     4981 gagctcgagc gcgagcttgc gcggcacgtc cctctgcacg atcgccgtga ccatgaacgg
     5041 ccacaggccg acgtcgatct cgggcatccc catctccgcg tcgtcgacgg cgacgacgag
     5101 gtcacacgcg agcatctggc cgaacccgcc ggcgagcgcg tgcccgttga cccgggcgac
     5161 gatcgggagc cgcgacgccc tcatcgcggc gaagagctcg ccgacctcgg ccctctcgcg
     5221 gtgctggccg accttcgagt cggcggtcat cccgcccagg tctcccccgg cgcagaacgc
     5281 cttgtcaccg gctccggtca ggacggcgac ccggacggag tcgtcggcct cggcccgggc
     5341 gagggccgcc ctcagggcgc gggtgacctc ggggttgatc gcgttgcgcc gctcggggcg
     5401 gtcgatcgtg agggtggcga cgccgccggc gacggcgtac gcgacgggac ctgcggtcaa
     5461 ccccgtccgg atctcaggat gtcgagcacg gcccgggcgt cttcgtcctc gatcgaggtc
     5521 gcgccgccgt cgaccggcct cggcgaggag ccgaacgcct tgcggaggtt ctcctcggcc
     5581 cgttcgaggg cctcctcggc cgccttcagc ctcacccgga cgtcggtctc gcggagcttc
     5641 gcctcttcga gctgctcctc gagctcctcg acccgcttgc ggtgggccgc gctctcggcg
     5701 gcgaaggcct cgacctgggc ggccctcagg ccgtcgtccc gggcgagctc gagcagccgg
     5761 gcgacgaccg ccttgccgag gtcgtcgtag ttgagccggg tcgtgcgctt cgccgcgcgg
     5821 ctcgggagca tggcctcggc gtcctcgagc ttgacgggat cggtcgacgc cagcttgatg
     5881 atcgtgttcc ggccccggcg gccccgctgg acgacctcga tccggcccag ctcctcgagg
     5941 agcttgatca cctcggcgct gcggacggag ttcagcccgg cctcggcggc gacggccctc
     6001 agcttgcctt cgaaagtgtc accctccgcg gaagaggcca gggtttcgag gagcctgttc
     6061 gccagctcgt ggaggttcgt tcgggccatg gggggctagc cgcttcctga gaggtaatag
     6121 ggacgcctaa tcttatccgg gcccccggaa taggcaaatg aatgaagcga agcctgaacg
     6181 ggctagatgg cctcgaggcc gcgggcgatg gcctcgatgc ggctggccgg gaagcgccgg
     6241 tggccgccga gggtgaccat gtgcgggagc ttgccgtcct tggcccagcg ggagacggtc
     6301 ttgggcgaga cccgcaagat gcgagccgcc tcggcggtct tcaagtaccg ctcgtcttct
     6361 ctgtcgatca actcaagcat tccgatcgct ccccaaaggg tccgtattgc ccgttgaggc
     6421 tctatcggcg gattggaggc attccttgaa gacctggcag gggggtcagg cgatgatcac
     6481 gtcgatcaac cggatgagtg ggtagtcggc catcacctgg aacaagagga gattggtctc
     6541 gccgaaggcg tgcctggctt cgggaggcgc cgtcgacaag gcgtcgcgga cgccggggta
     6601 gtaccgaaat ctgtatcgcc gcgccatggc gacgactcta ctcagagccc tagttgcctt
     6661 tcgagatcct cacggtcgag atcaggcccg tcgtcgcggc ccgcccaagc tacggcctcg
     6721 tcgtgatggg gctgctgtac gaactggagc gtgcgatacc aggcatccac cacgtccctg
     6781 actggcctta ggttgcccct tgcttgggcc tccaacagcg cctggacgat gtcccgggaa
     6841 aaggctcgcg ccccctcggt cgtcaaacga tcgaagacct cccgcgcctc ggatggaagg
     6901 cccagctcct cgggacgaac gatccggtcg ggtatggaag tcacagcgag cgctcctcat
     6961 gggcccatcc acgggtcgga cctgtaggca ccgaaccagt gtacggcccc acatgtgggg
     7021 cgcaaccttc acctggggat atggacgtcc gcgcgtgggc cggaccggcg gctacgcctc
     7081 ttctatgagc ccggcgattc ggtgcttcag cgcctcgggg cacgggtggg ccccccggag
     7141 gcgtgagatc agcgacttga cctcgacctc gaggccgtag cgctcgtagc aggggctgca
     7201 ctcctcgagg tgggcccgga tggacaggct ctccgcctcg gcgatcggct ccccgtcgat
     7261 cagcaggtag gcccgttcca gggtctccct gcagatctcc ttcatctcgt gcctaccatc
     7321 ggctcaggcc ccccggctcc cgtcactgga ggatcggacg atccccctgt ctcttgctag
     7381 gtcccacaac ctcttctgga gcgcctttct tccccggtgc agccgcgaca tgacggtccc
     7441 cagcgggacg tccatgatct cggccatctc ggcgtacgag aacccttcga cgtcggacag
     7501 gacgacggcc atccggaact gctcgggcag gtcgtcgatc gcctcgatga tgtccgtgtc
     7561 gaccagggag tcgaggacga tccgctccgg cgtctgcgag aactggtcgt cgtggttctt
     7621 gagctcctgg tacaggtcga agtcctcgac ctcgtcggcc gagaccttct tgggctcgcg
     7681 ctgcttcttc cggtaggtgt tgatgtacgc gttcgtcatg atccggaaca gccacgcctt
     7741 gaggttcgtc ccctcctcga agcggtcgaa ggagcggtag gcgcgcagca tggtctcctg
     7801 cactaggtcc tcggcgtcgg ccgggttccg ggtcatccgc agcgccgacg cgtagagcga
     7861 gtcgagcagc ggcagcgtgt cgcgcgtgaa ccgcgccttg tcggccggcg agagctcgac
     7921 ccttttcgcc tcagccaccg gccttccccc caccgaaccc tgcgagcagg ccggactcga
     7981 acgagaacat cagcttgccc ttcgcggcgt ggcgcgccag ggccgagtcg acgagcgacc
     8041 cgacgaggtc ccggaacgtg accccggacg cctcccacag gtagaacgcg aacgagcccg
     8101 gcacggtgtt gatctccatg acccatgtgt cgcccgtttc ccggttcacg aaactgtcga
     8161 tccgggcgac cccggcggcc cccacggcgg cgaacgcggc gagcgcgttg tcctggacct
     8221 gcttcgtcag ctcgtcgggg atgggggccg ggatgcggcg gtcctggctc gccatgccct
     8281 cgcccttcga cccgctcccc ttcccgccgc gcatgtactt gtcctcgaac gagaggaact
     8341 cgcgccacga cacgggctgc tcgcagaccg aagcccgagg agggagcccg gccccgccga
     8401 gcaccgagca gttcacctcc atgcagccct ccatcgcctt ctcgacgagg atccggtcgt
     8461 cgtagcgacg tgcgacgtcg agggcgtcgg cgagagcggc ccggtccggc gcacgcccga
     8521 tcccgacgct cgagccgagc cgcgacggct tgacgaacgc cgggtacccg atcgagccct
     8581 cgacccggtc gacgaccgcg tcgaggtccg cgtcgagctc ggcgacggcg acgacctcgt
     8641 gcggcacgac cggaagcccc gccgccgcga acaccgtctt catctcgacc ttgttcatcc
     8701 cgatcgccga cgcgagcacg tcgctccccg cgtacggcag gcccgccatc tcgagcaggc
     8761 cctgcagcgt gccgtcctcg ccgtacgtgc cgtggatcgc cgggatcacg acgtcgagcg
     8821 gtatccggcg cgggcccttg agcctcccgc cgggagcctg cagacccccg ccgtcgagcg
     8881 ccagcgcgac ctcctcgccg gcctcctcgt ggcggccctt cttgtagacg tcgaggtcgt
     8941 cgagggccgg ggacgagaac cagcggccgt ccctcgcgac gtagatcggg acgacgtcgt
     9001 agcggtcgcg ggccgcggcc atgacctggt gtgccgtgac gatcgagacg tcgtgctcga
     9061 ccgaccggcc cccgaagatc acgccgaccg tcagattccc tgccatcgcg cagagtctat
     9121 ggcgggaggt gcgtcatccc tcgtactggt cggggaggtc gttctcgaac agcacgacgt
     9181 cgccggggcc gacgaccttc gccagc
//
LOCUS       DASQDO010000175         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_690931, whole genome shotgun sequence.
ACCESSION   DASQDO010000175 DASQDO010000000
VERSION     DASQDO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690931"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VEV43_05185"
     CDS             complement(<1..304)
                     /locus_tag="VEV43_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HYP22946.1"
                     /translation="MAETIDFSVEGMTCGSCAARVQRSLEKVPGVVQADVNLATHKAR
                     VTGEPGLDPAPLVAAVDAAGYTLERIEPAQPPPEGPGTEAEHARAWRRRVIAVTPPA"
     gene            362..1804
                     /locus_tag="VEV43_05190"
     CDS             362..1804
                     /locus_tag="VEV43_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HYP22947.1"
                     /translation="MTPTFEERQRRLVEMLGSRSASDMTSGTLVVVPSITFPEAELRK
                     IVGIQLYEERLLFLLLLLRHPDLQLVFVTSLRVEEPIVDYYLRFVPADAEPGRRLWLL
                     ALWDPSPRALTGKLLEDPGAVARLRSLAEGESCLLTFNVTSLEARLADEVDAPLYGCP
                     PDLVGLGSKSGSRRVAREAGVAVPPGAEDVHSPHEVEKALNELKLRSPAARSAVVKLN
                     DGFSGQGNAIVDLGGDAPLEERPTVFCAQEESWPSFTEKLREQGGVVEELMKVPGMAS
                     PSVQMRIAPDGSFEVVSTHDQILGGPDDQVYLGCRFPADPRYRSAIADAGRRVARVLA
                     ERGVIGSFGIDFVIAPGEEAEPFLTEINLRMGGTTHPFLMTEFATGGTYDEATGELVV
                     DRRAKHYVATDNLKSDAYVGLLPEALIAAIDRAGLAFDATTNTGVLLHLLGALKRYGK
                     VGMTCVADSASDADALFEMATGVLERLGTR"
     gene            complement(1814..>2449)
                     /locus_tag="VEV43_05195"
     CDS             complement(1814..>2449)
                     /locus_tag="VEV43_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22948.1"
                     /translation="ECGVEATGKFCWSCGAALRAGASPAAAPPAPAVAWDPNEPSYAA
                     VVARPGVRELLAAEADAADNPMTAEEFLSTCQKVLPQPVPLVPLYEIVGPLYGRLGIK
                     TGRTEKRSVAVPAGVALAGVLASLARHGNDIRRVEQATDGCVVHAELSSSMWHGKGDI
                     VVTVEQDGAGASVEAATKIPGQVFDWGRSKVRLDALFEDLLDDRVRAATGV"
BASE COUNT          330 a          837 c          919 g          363 t
ORIGIN      
        1 cggccggggg cgtgacggcg atcacgcggc ggcgccaggc gcgggcgtgc tcggcttcgg
       61 tcccgggccc ctccggcggg ggctgcgcgg gctcgatgcg ctcgagcgtg tatccggcgg
      121 cgtcgacggc cgcgaccagg ggggccgggt cgagccccgg ctcgccggtg acgcgcgcct
      181 tgtgcgtcgc gaggttgacg tctgcctgca cgacgccggg caccttctcg agggaccgct
      241 gcacgcgggc cgcgcaggac ccgcaggtca taccttcgac gctgaaatcg atcgtctcgg
      301 ccatggccct acgatacccc cccagggtat ctccggtcaa cccccggcgt agtctgccgc
      361 gatgacgccg acgttcgagg agcggcagcg ccgcctcgtc gagatgctgg ggagccgcag
      421 cgcgtccgac atgacgtcgg gcacgctcgt ggtcgtcccg tcgatcacgt tccccgaggc
      481 cgagctgcgc aagatcgtcg gcatccagct ctacgaggag cgcctgctct tcctcttgct
      541 gcttctgcgc catccggacc tgcagctcgt cttcgtgacg tcgctgcggg tcgaggaacc
      601 gatcgtcgac tactacctgc ggttcgtgcc ggccgacgcg gaaccgggcc ggaggctgtg
      661 gctgctcgcg ttgtgggacc cgtcgccgcg cgcgctgacg gggaagctcc tggaggaccc
      721 cggcgcggtg gcgcgcctcc gttcgctcgc cgagggcgag tcgtgcctgc tgacgttcaa
      781 cgtcacgtcg ctcgaggccc ggctcgcgga cgaggtcgac gcccccctct acgggtgccc
      841 tccggacctc gtcgggctcg gctccaagtc cgggtcgcgg cgggtcgcac gggaggcggg
      901 cgtcgcggtc ccgccggggg ccgaggacgt ccactcgccg cacgaggtgg agaaggcgct
      961 gaacgagctg aagctgcgct cccccgcggc gcggtcggcc gtcgtgaagc tgaacgacgg
     1021 cttctcgggg cagggcaacg cgatcgtgga cctcggaggc gacgcaccgc tcgaggagcg
     1081 accgacggtc ttctgcgcgc aggaggagtc gtggccgtcg ttcaccgaga agctgcggga
     1141 gcaaggtggc gtcgtcgagg agctgatgaa ggtgcccggg atggcgtcgc cgagcgtgca
     1201 gatgcggatc gcaccggacg ggagcttcga ggtcgtgtcg acgcacgacc agatcctcgg
     1261 gggccccgac gaccaggttt atctggggtg ccggttcccc gccgatccgc gctaccgctc
     1321 ggcgatcgcg gacgcggggc gacgcgtcgc tcgcgtcctg gcggagcgcg gcgtgatcgg
     1381 ctccttcggg atcgacttcg tcatcgctcc gggcgaggag gccgagccgt tccttacgga
     1441 gatcaacctg cgtatgggag ggacgaccca tccattcctg atgacggagt tcgcgaccgg
     1501 gggtacctac gacgaggcta cgggtgagct cgtcgtagac cgccgggcga agcactacgt
     1561 ggcgacggac aacctgaagt ccgacgcgta cgtggggctg ctgccggagg cgttgatcgc
     1621 ggcgatcgac cgcgctgggc tcgcgttcga cgcgacgacg aacaccggcg tcctcttgca
     1681 tctgctcggc gcgctgaaga gatacggcaa ggtcgggatg acgtgcgtcg cggactcggc
     1741 gtccgacgcg gatgcgctgt tcgagatggc gaccggcgtg ctcgaacggc tcggcacgcg
     1801 gtagcgcggg ccgtcagacg cccgtcgcgg ccctcacgcg atcgtcgagc agatcctcga
     1861 acagcgcgtc gaggcggacc ttgctgcgcc cccagtcgaa gacctgtccc gggatcttcg
     1921 tcgccgcctc gaccgacgca cccgcgccgt cctgctcgac cgtgacgacg atgtcgccct
     1981 tcccgtgcca catcgacgag ctcagctccg cgtgcacgac gcatccgtcc gtcgcctgct
     2041 cgacgcggcg gatgtcgttg ccgtggcgag cgagcgacgc gaggacgccg gcgagagcga
     2101 cgccggcggg gaccgccaca gaccgtttct cggtgcggcc cgtcttgatc ccgaggcggc
     2161 cgtagagggg gccgacgatc tcgtagagcg ggacgagcgg caccggttgc gggaggacct
     2221 tctggcaggt cgagaggaac tcctcggcgg tcatggggtt gtcggccgcg tcggcctcgg
     2281 cggcgaggag ctcgcggacg ccgggccgag cgacgacggc ggcgtagctg ggctcgttgg
     2341 ggtcccaagc cacggcgggg gcgggtggcg cggcggcggg cgaggccccg gcacgcaagg
     2401 ccgcgccgca gctccagcag aacttgccgg tcgcctcgac cccacactc
//
LOCUS       DASQDO010000176         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_17758, whole genome shotgun sequence.
ACCESSION   DASQDO010000176 DASQDO010000000
VERSION     DASQDO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17758"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..854)
                     /locus_tag="VEV43_05200"
     CDS             complement(42..854)
                     /locus_tag="VEV43_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HYP22949.1"
                     /translation="MHPLVVTVAAVGAELTLDDQPNLPVTPELLAQDAAECAAAGASI
                     YHLHVRDDEGRPTMSVDRFRAARAAIEDASDLIVQFTTGGAVTDPIEARLAPLDLAPE
                     MATLTTGTVNFGDEVFSNPVPLVSRLYRRMIEQGVTPEYEIFDAGMIATATRLREELG
                     AGHHPHFDFVLGVPGALPAWDDALPFLVSHLPEGATWSATGIGRAHLPVTEQTIALGG
                     HVRTGFEDVLYYAKGVKARSNAQLVERVTDMARSAGREVATPAITREMLGLR"
     gene            complement(860..1879)
                     /locus_tag="VEV43_05205"
     CDS             complement(860..1879)
                     /locus_tag="VEV43_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYP22950.1"
                     /translation="MRLSAAALLLAATAVPVLAGAAASEETPEDLKARMTNIQAELDA
                     TTQELEDLHAQEESLATELEDIESKVAVLEKQEAKLAVVVARRADELYRAGSTYLVEV
                     LIDSESIAELSTKAEMLSHISMDDSNAFLDMALTRAELEHYEEQLRVKRAALADTEED
                     YLAKSEELQAEFDSVSAELERIQRALAAVQPSAPSAPAPVTSTVSSVPSTNGKACPVG
                     QPHSFIDSWGYPRSGGRSHEGVDMMAGYGTPVYAIVSGSITYAGYGDSAGNWQILSGS
                     DGNAYWYMHNQSNIVTGGSVKAGQQIATVGDTGNATGVPHLHFEYHPGGGGPVNPYPL
                     VAAIC"
     gene            1969..2604
                     /locus_tag="VEV43_05210"
     CDS             1969..2604
                     /locus_tag="VEV43_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYP22951.1"
                     /translation="MRGVEDYLEEIKRLEEDGDRITATVLARTLEVSLPSASEMLKRL
                     AGEGYLVREKGGSIVLTPDGRRLAHTILRRHRLVECLLVQKLGMEWYEVHGEAHRIEH
                     AISARVEEAMAKALDYPNYCPHGHPICVVDYRDLRRLSETEAGGTGVVAQISEVKEEV
                     LPYFDQNGIRPGATLHVKDIAPMDGPITVETPDGVTSIGRELARLVRLSIP"
     gene            complement(2601..>3389)
                     /locus_tag="VEV43_05215"
     CDS             complement(2601..>3389)
                     /locus_tag="VEV43_05215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYP22952.1"
                     /translation="GGTTGPGAILGAIVLVVGAPVIEEAYFRGMLYERFARWGLASAV
                     IGTSLLFTVVHGVGIWDPPRLLLGFALGFARRSKSLWFTIAGHAAWNGAIVFIALFMM
                     TGGGHNFTSVDGSFSLRHPAEWERLEQAEIQTPQGNMEVLLTTPEGSMMGVMSMQAPH
                     ALNRWNLMQVAQQAQGSFPMPPGATMSPLQETHLIRDPLVTAYESKIRISDPMMGTGN
                     GRVVLVVRDGSKSVLMLIMGCPDVDCAEAEPEFDSMLKSLSFTA"
BASE COUNT          527 a         1141 c         1203 g          518 t
ORIGIN      
        1 tcgacgaggc ggcgcttctg atcggtcatg aggtcagggt atcagcggag tcccagcatc
       61 tcgcgggtga tcgcaggcgt cgcgacctcg cgcccggcgc tccgcgccat gtccgtcacc
      121 cgctcgacca gctgcgcgtt cgaccgcgcc ttgacgccct tcgcgtagta gaggacgtcc
      181 tcgaagcccg tgcgcacgtg gccgccgagg gcgatcgtct gctccgtcac cggcaggtgc
      241 gctcggccga tgccggtagc gctccacgtg gcgccctcgg gcagatggga gacgaggaac
      301 gggagcgcgt cgtcccacgc cgggagcgcc cccgggacgc cgagcacgaa gtcgaagtgc
      361 ggatggtgac cggcccccag ctcctcccgc agccgggtcg cggtcgcgat catcccggcg
      421 tcgaagatct cgtactcggg cgtcacacct tgctcgatca tccggcggta gagacgcgac
      481 accagcggga ccgggttcga gaacacctcg tcgccgaagt tcacggtccc ggtcgtcagc
      541 gtcgccatct cgggagcgag gtccaagggc gcgagccggg cctcgatcgg gtcggtcacc
      601 gcgcctccgg tcgtgaactg cacgatcagg tcgctcgcgt cttcgatcgc cgcacgggcg
      661 gcccggaagc ggtcgaccga catcgtcgga cggccctcgt cgtcgcgcac gtggaggtgg
      721 tagatcgagg cgccggcggc ggcgcactcg gccgcgtctt gcgcgagcag ctcgggcgtg
      781 acggggaggt tcggctggtc gtcgagcgtc agctccgcgc cgacggcggc gacggtgacg
      841 acgagcggat gcacggtcgc tagcagatgg cggctacgag cgggtacggg ttcaccggcc
      901 cgccgccgcc cgggtggtac tcgaagtgca ggtgcgggac gccggtcgcg ttgccggtgt
      961 cgccgacggt tgcgatctgc tggccggcct tcacggaccc gccggtcacg atgttgctct
     1021 ggttgtgcat gtaccagtac gcgttgccgt cgctgcccga gaggatctgc cagttcccgg
     1081 cgctgtcgcc gtacccggcg taggtgatcg agccggagac gatcgcgtat accggggtgc
     1141 cgtagcccgc catcatgtcg acgccctcgt ggctccgccc gcccgaccgg gggtagcccc
     1201 agctgtcgat gaacgagtgg ggctggccga cggggcacgc cttgccgttg gtcgagggta
     1261 ccgacgaaac cgtcgaggtc acgggggccg gcgccgacgg tgcgctcggc tgcaccgcgg
     1321 cgagcgcccg ctggatccgc tcgagctccg cggagaccga gtcgaactcg gcctgcagct
     1381 cctctgactt cgcgaggtag tcctcttcgg tgtccgcgag ggcggcccgt ttgacgcgca
     1441 gctgctcctc gtagtgctcg agctcggccc tcgtgagcgc catgtccaag aacgcgttcg
     1501 agtcgtccat ggagatatgg gacaacatct cggccttggt agagagctcg gcgatgctct
     1561 cggagtcgat caggacctcg acgaggtagg tcgacccggc ccggtagagc tcgtctgccc
     1621 ggcgggcgac gacgaccgcg agcttggcct cctgcttctc gagcacggcg accttcgact
     1681 cgatgtcctc gagctcggtg gcgaggctct cctcctgagc gtggaggtcc tcgagctcct
     1741 gtgtggtcgc gtcgagctcg gcctggatgt tggtcatccg ggccttcaag tcctcgggcg
     1801 tctcctcgga ggcggccgca ccggcgagaa cgggcacggc ggtcgcagcg aggaggaggg
     1861 cggcggcgga cagacgcacc gctcgggcaa tccttcggtt tcgcaccagc cggggaccct
     1921 agcagatggg gcaaaagtgc aggtcaagcc gctagtacac tcgtgcaaat gaggggcgtc
     1981 gaggactacc tggaggagat caaacggctc gaggaagacg gcgaccgcat caccgccacc
     2041 gtcctcgctc ggaccctcga ggtctcgctc ccgtcggcct ccgagatgct caagcggctc
     2101 gccggcgagg gctacctcgt ccgggagaag ggcggctcga tcgtcctcac gcccgacgga
     2161 cgacggctcg cccacacgat cctgcgacgc caccggctcg tcgagtgcct gctcgtccag
     2221 aagctcggca tggagtggta cgaggtccac ggcgaggccc accgcatcga gcacgcgatc
     2281 tcggcccggg tcgaggaggc gatggccaag gccctcgact acccgaacta ctgcccgcac
     2341 ggccacccca tctgcgtcgt cgactaccgc gaccttcgaa ggctgagcga gaccgaggcc
     2401 gggggcacgg gcgtcgtggc gcagatctcc gaggtgaagg aagaggtcct cccctacttc
     2461 gaccagaacg ggatcaggcc gggggcaacc ctgcacgtga aggacatcgc gccgatggac
     2521 ggaccgatca cggtcgagac gccggacggc gtgacgtcga tagggcgtga gctcgcgcgg
     2581 ctggtgaggc tgtcgatccc ctaggccgtg aagctcagcg acttgagcat cgagtcgaac
     2641 tcgggctccg cctccgcgca gtcgacgtcg gggcagccca tgatcagcat caacacggac
     2701 ttcgagccgt cgcgcaccac gagcacgacc cggccgttgc ccgtgcccat catcgggtcc
     2761 gagatccgga tcttcgactc gtaggccgtc acgagggggt cccgaatgag gtgggtctcc
     2821 tgcagagggc tcatcgtcgc gcccggaggc atcgggaagg agccctgcgc ctgctgcgcg
     2881 acctgcatga ggttccagcg gttgagcgcg tgcggcgcct gcatcgacat cacgcccatc
     2941 atcgagccct ccggcgtcgt cagcaggacc tccatgttcc cctgaggcgt ctggatctcg
     3001 gcctgctcga ggcgttccca ctcggccggg tggcggagag agaacgaccc gtcgacagag
     3061 gtgaagttgt ggcccccgcc ggtcatcatg aacagggcga tgaagacgat cgcgccgttc
     3121 cacgcggcgt gtcccgcgat cgtgaaccac agcgacttgc tccggcgggc gaacccgagg
     3181 gcgaagccga ggaggagccg gggcgggtcc cagatgccga cgccgtgcac gaccgtgaag
     3241 agcagcgacg tgccgatgac tgcggaggcg aggccccagc gagcgaagcg ctcgtagagc
     3301 atcccgcgga agtaggcctc ttctattacc ggggcgccga cgacgaggac gatcgccccg
     3361 aggatggctc ccgggcccgt ggtgccgcc
//
LOCUS       DASQDO010000177         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_446359, whole genome shotgun sequence.
ACCESSION   DASQDO010000177 DASQDO010000000
VERSION     DASQDO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446359"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VEV43_05220"
     CDS             complement(<1..165)
                     /locus_tag="VEV43_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HYP22953.1"
                     /translation="MAKIKDDDIDALRERADLVEVVSAHSQLKRAGSHTFKGLCPFHS
                     EKTPSFQVDAA"
     gene            complement(243..1208)
                     /locus_tag="VEV43_05225"
     CDS             complement(243..1208)
                     /locus_tag="VEV43_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HYP22954.1"
                     /translation="MKGTWRERIEAAERERLSERAQVASESKGRDRFEPEDDLRTCYQ
                     RDRDRIIHSKAFRRLAHKTQVFLAPEGDHYRVRLTHTLEVTQVARTIARALALNEDLV
                     EAVCLGHDLGHTPFGHLGEEVVAAFHPGFRHNLQSVRIVEVLEDLNLTWEVRDGIANH
                     TWSMPMPATLEGQIARYADRIAYLNHDIDDSLRAGILTEADLPEVTTAVLGATHSDRI
                     ETMVTDLVAVSEGAEAISMSPPVFEAMSATRKFLFERVYVGRVGAATREAVERILQTL
                     LAHYGSGADDPQAAVDYVAGMTDRFALRAFERLVEGPAPGLGVLQ"
     gene            1258..1923
                     /locus_tag="VEV43_05230"
     CDS             1258..1923
                     /locus_tag="VEV43_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22955.1"
                     /translation="MGTRLRGPLAALLSLVLVAGLAAPAAGQLDARSEPAVTGPLTAF
                     GDGKRCRATKYEVEGEIVARAKICLFLYALDPDQEDDEEDDYGVMWAQTNVDTSGGWC
                     AFRVHTEVYIPSAMPMYASVPKAQKITKRKTVRWKLTVDAANQAAEAATVKKGLTLYP
                     DSLRRSRRASDDEGVDIVRLTWRGETARKLGFVSGFEVSWKEDDGPPDAVPFRVEYPI
                     REC"
     gene            complement(1937..2839)
                     /locus_tag="VEV43_05235"
     CDS             complement(1937..2839)
                     /locus_tag="VEV43_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl endopeptidase"
                     /protein_id="HYP22956.1"
                     /translation="MNSGLTIGIYGGGPLYSGGPGVIDDLRASGFTTVVAWSVHVETN
                     GDLVFNDPPIVQGGKYVGNPGWPAQLAALKQPPTSVNRLIFSVGAGGVQDFTNVAKLI
                     ASQGTGPSSVLYRNFAALRQTIPEIDAIDFDDEDLYDLPTIVKFAQMLHEIGFSVTFC
                     PWTDVGFWTKCLKALNTPTDHVVTQFNLQCYSGGAGNDPSTWIKAVGAAMGPSFDAKG
                     FVFPGLGAALWDSKAKTCTMPNRDGYYCPADLGGRLAGWRTTGIQGGWVWLLDAVLNC
                     QTSGICRGAMGTAAYAAAVRAALT"
BASE COUNT          460 a          991 c         1015 g          465 t
ORIGIN      
        1 cgccgcgtcg acctggaacg acggcgtctt ctcggagtgg aaggggcaga gccccttgaa
       61 cgtgtgcgag ccggcgcgct tgagctggga atgggccgag acgacctcga cgaggtcagc
      121 gcgctcgcgc agagcgtcta tgtcgtcgtc tttgatcttg gccatcccac ccccttgggc
      181 gtctcgccga ggcgacccgc gcctccttac gggcgcggct tacgaaggaa ggggaagtgg
      241 actcattgta ggacccccag cccgggggcc ggcccctcga cgaggcgctc gaacgcccgc
      301 agggcgaaac ggtcggtcat gcccgcgacg tagtccacgg ccgcctgagg gtcgtcggcc
      361 ccggagccgt agtgcgccag caaggtctgg aggatccgct cgacggcctc gcgggtcgcc
      421 gcgcccacgc gcccgacgta gacccgctcg aacaggaact tgcgcgtggc gctcatggcc
      481 tcgaagaccg gggggctcat cgagatcgcc tcggccccct cggacacggc cacgagatcg
      541 gtgaccatcg tctcgatccg gtcggagtgc gtcgcgccga gcaccgccgt cgtcacctcc
      601 ggcaggtcgg cctcggtcag gatgccggcg cgcagtgagt cgtcgatgtc gtggttgagg
      661 taggcgatgc ggtcggcgta gcgcgcgatc tggccctcga gcgtcgccgg catcggcatc
      721 gaccacgtgt ggttggcgat cccgtcccgc acctcccacg tcaggttgag gtcctcgagc
      781 acctcgacga tccgcaccga ctggaggttg tggcggaagc cgggatggaa cgccgcgacg
      841 acctcctcgc cgagatggcc gaacggagtg tggccgaggt cgtgcccgag acagacggcc
      901 tcgaccaggt cctcgttcag cgccagcgcc cgggcgatcg tgcgcgctac ctgcgtgacc
      961 tcgagcgtgt gcgtcagccg gacccggtag tggtccccct ccggcgcgag gaacacctgg
     1021 gtcttgtggg cgagccgccg gaaggccttg ctgtggatga tccggtcgcg gtcgcgctgg
     1081 tagcaggtgc gcaggtcgtc ctcgggctcg aaccggtcgc gccccttgga ctcggaagcg
     1141 acctgggcgc gctcggacag ccgttcgcgt tcggctgcct cgattcgttc acgccaggtg
     1201 cctttcatgc gaaggacgct agcattgggg tacgacggca tatcgagggg gcgaactgtg
     1261 ggcacgaggt tgcgcggccc cctggcagcg ttactgagtc tggtcctggt cgcggggctc
     1321 gcagcccccg ccgccgggca gctcgacgct cggtccgagc ccgccgtcac gggccccctg
     1381 actgcgttcg gcgacggcaa gaggtgccgt gcgacgaagt acgaggtcga gggcgagatc
     1441 gtcgcccggg cgaagatctg cctgttcctc tatgcactcg atcccgacca ggaggacgac
     1501 gaagaagacg actacggcgt gatgtgggcg cagacgaacg tcgacacgtc gggcgggtgg
     1561 tgcgccttcc gcgtccacac cgaggtctac atcccgtcgg caatgcccat gtacgcgagc
     1621 gtcccgaagg cgcagaagat aacgaagcgg aaaacggtca ggtggaagct cacggtcgat
     1681 gcggcgaacc aagccgctga ggccgccacg gtgaagaaag gcctgacgct gtaccccgat
     1741 tccctcaggc gttcgcgacg ggcgagcgac gacgaagggg tggacatcgt caggctgacg
     1801 tggagaggag agaccgccag gaagctcggc ttcgtgtcgg gattcgaggt gtcgtggaaa
     1861 gaagacgacg gccccccgga cgcggtgccg ttccgggtgg agtacccgat ccgggagtgc
     1921 tgagcgttcg cgggcgctat gtcagcgcgg ccctcaccgc tgcggcgtag gccgccgtcc
     1981 ccatcgcccc ccggcagatg cccgaggtct ggcagttgag caccgcgtcg agcagccaca
     2041 cccagccgcc ctggatcccg gtggtccgcc agccggccag cctcccgccc aggtcggccg
     2101 ggcagtagta gccgtcccgg ttgggcatcg tgcacgtctt cgctttggag tcccatagcg
     2161 ccgcgcccag gcccgggaag acgaacccct tcgcgtcgaa cgacggtccc atcgcggccc
     2221 ccacggcctt gatccacgtc gagggatcgt tgccggcgcc gccggaatag cactgcaggt
     2281 tgaactgcgt cacgacatgg tccgtcgggg tgttcagcgc cttgaggcat ttggtccaga
     2341 accccacgtc cgtccaagga cagaacgtca ccgagaaacc gatctcgtgc agcatctgcg
     2401 cgaacttcac gatcgtcggc aggtcgtaga ggtcttcgtc gtcgaagtcg atcgcgtcga
     2461 tctccgggat cgtctgcctc agagccgcga agttccggta gaggacgctg gacggccccg
     2521 tcccctggct cgcgatcagc ttcgccacgt tcgtgaagtc ctggacgccg cccgctccca
     2581 ccgagaagat caaccggttc acgctcgtcg gcggctgctt cagcgccgcg agctgcgccg
     2641 gccaaccggg gttcccgacg tacttcccac cctggacgat cggggggtcg ttgaagacga
     2701 ggtctccgtt ggtctcgacg tggaccgacc aggcgaccac ggtcgtgaac ccggaggcgc
     2761 gcaagtcgtc gatgaccccg gggccgcccg aatacagcgg gccgccaccg tagatgccta
     2821 tcgtcaggcc gctgttcatc cgtccgccct ccccccggag ttgcctactt cgacgaggtc
     2881 gtcgcctctc ccatcactgc ttgggcggcc gcgaggcggg ccgtcgggac c
//
LOCUS       DASQDO010000178         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_263073, whole genome shotgun sequence.
ACCESSION   DASQDO010000178 DASQDO010000000
VERSION     DASQDO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263073"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEV43_05240"
     CDS             complement(<1..374)
                     /locus_tag="VEV43_05240"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HYP22957.1"
                     /translation="MQPIDPARFREVPTLTGPEVSRRAGVEHAFAKRVWLTLGLPDIP
                     DDEVEFDESDVDVLGGLKLILDQGYPREDVLDVARAYGQAMSRLAQAEVRLFNKVFLA
                     ALREQSADDDEMAGRLEAVVPA"
     gene            595..1716
                     /locus_tag="VEV43_05245"
     CDS             595..1716
                     /locus_tag="VEV43_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22958.1"
                     /translation="MRKCVRIASIVAVIGGVLFTNHAQAVAPKLELTEFTVDEKALCE
                     LRDQWIDDLLKTHEHGTWATMEMELTDAQLEKVGLPPADVLRAGDYSQPTLVTNDGEY
                     VDVPLEELETEVDASVATFAGTGCLGIRPGSLLLSVTNDSIGWCTLAHAYGSPGAYDI
                     STAGHCGNTGDTATVIAGMGNRADATGVVLLDFGRYARSTGDGGLGNDHALIDIDPAY
                     QSLVTPTMCFWGGPRGRFDSTGEVAKVGFESNNLLRPQVTTDPNPALAQTIVHYGHGT
                     GVGAGGTPRVGEAIAWRADHFMFTGAINLGDSGSGANTLLGDAVGDNMEAAGILTHIW
                     VDPLMRDGIGIMGGTRASLVGTPANGQIVPWPAPVPGLP"
BASE COUNT          301 a          578 c          602 g          248 t
ORIGIN      
        1 aacgcgggga cgacggcttc gagccggccc gccatctcgt cgtcgtccgc ggactgttct
       61 cgcagcgcgg cgaggaacac cttgttgaag aggcgcacct cggcctgagc gaggcgcgac
      121 atcgcttggc cgtacgcccg cgcgacgtcg agtacgtcct cgcgcgggta gccctggtcg
      181 aggatcagct tcaggccccc cagcacgtct acgtcgctct cgtcgaactc gacctcgtcg
      241 tcggggatgt cggggagacc gagcgtgagc cacacgcgct tcgcgaacgc gtgctcgacg
      301 cccgcgcggc gcgagacctc ggggcccgtg agcgtcggga cctcgcggaa gcgcgccgga
      361 tcgatgggct gcatcgccgc gacgctaaca acgcagcgtt gacgaccgcg cgcagagagc
      421 atcgagctct atctccatgt cggcgcgcga ctccggcgtg cagatgcttg tttaccttaa
      481 ccggacaaat cgtaaagaaa cgtgacaaat cgtgcaggag gctccgtacg aggggcgaag
      541 gtcgggggag cgctgtttac cggcagccgc gcaagccgac cgagaggaga gcccatgcgg
      601 aagtgtgtcc ggatcgcttc gatcgttgcg gtgatcggtg gtgtcttgtt caccaatcac
      661 gcgcaagcgg tggcaccgaa gctcgagctg acggagttca ccgtcgacga gaaagctctg
      721 tgcgagctac gcgaccagtg gatcgacgac ttgctgaaga cccacgagca cggtacgtgg
      781 gcgacgatgg agatggagct caccgacgct cagctcgaga aggtcggcct tccccccgcg
      841 gacgtgctcc gcgccggcga ctactcgcag ccgacgctcg tcacgaacga cggcgaatac
      901 gtcgacgtcc cgctcgaaga gctcgagacc gaggtcgacg cgtcggtggc gacgttcgcc
      961 gggaccgggt gcctcgggat ccgtccaggc tcgctgctcc tgtcggtcac gaacgacagc
     1021 atcggctggt gcacgctcgc acacgcgtac ggatcgcccg gcgcctacga catcagcacg
     1081 gccggacact gcggcaacac cggagacacc gcgaccgtga tcgccgggat gggcaaccgc
     1141 gcggacgcca ccggcgtagt gctgctcgac ttcggccgct acgcacgcag cacgggtgac
     1201 ggggggttgg gcaacgacca cgcgttgatc gacatcgatc ccgcgtacca gagcctcgtc
     1261 acgccgacga tgtgcttctg gggcgggccg cgcggccgct tcgactcgac cggcgaggtg
     1321 gcgaaggtcg gcttcgagag caacaacctg ctccggccgc aggtcaccac cgaccccaac
     1381 cccgcgctgg cgcagacgat cgtccactac gggcacggca cgggtgtggg cgcgggcggc
     1441 acgccgcgtg tcggcgaggc gatcgcgtgg agagcggacc acttcatgtt caccggcgcg
     1501 atcaacctcg gcgactccgg ctcgggcgcg aacacgctgc tcggcgacgc ggtcggcgac
     1561 aacatggagg cggccgggat cctcacgcac atctgggtcg accctctgat gcgcgacggc
     1621 atcggcatca tgggaggcac ccgagcgtcc ctggtgggga cgccggcgaa cggacagatc
     1681 gtcccgtggc cggcgccggt gccgggactg ccgtaaccgg aagagtccg
//
LOCUS       DASQDO010000179         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1180749, whole genome shotgun sequence.
ACCESSION   DASQDO010000179 DASQDO010000000
VERSION     DASQDO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180749"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VEV43_05250"
     CDS             complement(<1..227)
                     /locus_tag="VEV43_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HYP22959.1"
                     /translation="MHRSLEPRGVLPQQARVLDTSLPLRRGEALIDVEYLNVDSASWR
                     QLREEAGGDAAVMKARIESIVASAGKMHNPVT"
     gene            complement(247..1644)
                     /locus_tag="VEV43_05255"
     CDS             complement(247..1644)
                     /locus_tag="VEV43_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HYP22960.1"
                     /translation="MTKRLERDKEILRRGGRAHAAEQPYVYPPAREWAEPDWTRIPGF
                     REVTPEQWESARWQRQHTIKNLGQLKAALGDLLPADLEESIARDLAERATMSMLVPPQ
                     MLNTMDTSDLWRDPVRRYMIPALADRDPEWPSHPLAERDSLHEADMWAVEGLTHRYPT
                     KVLAELLSTCPQYCGHCTRMDLVGNSVPQVLKYRFELKQKERYGAILDYLRVTPSVRD
                     VVVSGGDVANLPIAHLEEFVSALLDIENIRDVRLATKGLMGLPQHFLQDDVLAGMERM
                     ATKALDRGVDLAIHTHVNHAASLTPLVAKAVAKLRAMGFRDVRNQGVLLRGVNDSSHA
                     LLDLCFTLLDRAKITPYYFYMCDMIPNSEHWRVPLHEAQALQHSIMGYLPGFATPRIV
                     CDVPFVGKRWVHQVDSYDRVRGISYWTKNYRTGIEGGDADALSRTHAYYDPVPSLPAE
                     GRQYWRDHVLQSAPA"
     gene            1721..2179
                     /locus_tag="VEV43_05260"
     CDS             1721..2179
                     /locus_tag="VEV43_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYP22961.1"
                     /translation="MDSTDRRIVALLEKDARLTYGEVGKRVGLAASSVHDRVRKLEKK
                     GVLRGYRADVAFSSVGLPITAFVSLALRPSSPADVPARVAEFPLVESCYSVAGDNSYV
                     LVVRAPTTSALEELLDALRAKLEVVTRSTIVLSTPFERRPMTAEGNSSRP"
     gene            2286..>3379
                     /locus_tag="VEV43_05265"
     CDS             2286..>3379
                     /locus_tag="VEV43_05265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYP22962.1"
                     /translation="MTTFTELGVSARVVRALEHRGITQPFEIQALVIRDAIAGRDVLA
                     KSRTGSGKTLGFGIPVVERLEPGPKRPSALILVPTRELALQVATEVEAIATARSLRVT
                     AVYGGAGLQAQARAAGRADILVATPGRLQDLLDRRAISLRSIQILVLDEADRMLDMGF
                     QPQVDRILRDAPQQRQTMLFSATLDGVVGRLARQYTKDPATHEVHSSKPTVEEADHRF
                     VTAAKEARADVLASLLRDEDGATLVFVRTKRGADRLAKRLTRAGHDAVAMHGDLSQGQ
                     RERALAKFSSGRARVLVATDVAARGLDVDLISHVVNFDPPHDGDTYVHRVGRTARAGR
                     SGLGTTLVMPDQAAEGAKWAGELGLHDALA"
BASE COUNT          503 a         1174 c         1154 g          548 t
ORIGIN      
        1 gtcaccgggt tgtgcatctt gccggccgag gcgacgatcg actcgatgcg cgccttcatc
       61 acagccgcgt cgccgccggc ctcctcgcgc agctggcgcc acgacgccga gtcgacgttc
      121 aggtactcga catcgatcag agcttccccc cggcgcaacg gcagcgaggt gtcgagaacg
      181 cgcgcctgct gcggcaggac gccgcgcggc tccaacgaac ggtgcacgcc gtaacgctcc
      241 atgggcttaa gccggcgcgg actgaaggac gtggtcccgc cagtactggc gcccctctgc
      301 gggaaggctc ggcaccgggt cgtagtaggc gtgcgtccgg gacagcgcgt ccgcgtctcc
      361 gccctcgatg cccgtgcggt agttcttcgt ccagtacgag atcccgcgca cccggtcgta
      421 agagtcgacc tggtggaccc agcgcttgcc gacgaacggg acgtcgcaga cgatccgcgg
      481 cgtggcgaac ccgggcaggt agcccatgat cgagtgctgc agcgcctggg cttcgtggag
      541 cgggacccgc cagtgctcgg agttcgggat catgtcgcac atgtagaagt agtacggcgt
      601 gatcttcgcc cggtcgagca aggtgaagca caggtcgagc agcgcgtgcg acgagtcgtt
      661 gaccccgcgc agcaacacgc cctggttgcg gacgtcgcgg aaccccatcg cccggagctt
      721 cgcgacggcc ttcgcgacca gaggagtaag cgacgccgca tggttcacgt gcgtgtggat
      781 cgcgaggtcc actcccctgt cgagcgcctt cgtcgccatc cgctccatcc ccgcgagcac
      841 gtcgtcctgg aggaagtgct gcggcagtcc catcagaccc ttggtcgcga gcctgacgtc
      901 gcggatgttc tcgatgtcga gcagcgcgct cacgaactcc tcgaggtgcg cgatcggcag
      961 gttcgcgacg tcaccgccgg acacgacgac gtcgcgcacg ctcggcgtga cccggaggta
     1021 gtcgaggatc gctccgtagc gctccttctg cttgagctcg aagcggtact tgagcacctg
     1081 cgggacgctg ttgccgacga ggtccatccg cgtgcagtgc ccgcagtact gggggcaggt
     1141 cgagagcagc tcggcgagga ccttcgtcgg gtagcggtgg gtgaggccct cgaccgccca
     1201 catgtcggcc tcgtgcaggc tgtcgcgctc ggcgaggggg tggctcggcc actcgggatc
     1261 ccggtcggcc agggccggga tcatgtagcg ccggaccggg tcgcgccaca ggtccgacgt
     1321 gtccatcgtg ttgagcatct gcggcgggac gagcatcgac atcgtcgccc gctcggccag
     1381 gtcccgggcg atcgactcct cgaggtcggc cggcagcagg tcgccgagcg cggccttcag
     1441 ttgaccgagg ttcttgatcg tgtgctggcg ctgccagcgg gcgctctccc actgctcggg
     1501 agtgacttca cggaagccgg ggatgcgcgt ccagtccggc tcggcccatt cccgggcggg
     1561 cggatagacg tagggctgct cggccgcgtg ggcgcgcccc ccccggcgca agatctcctt
     1621 gtccctctcg agcctcttgg tcactccggg tcctccgctg tattctccaa atgacggaac
     1681 gatattaggc agaatagctg atctgcggaa ggatgttcgg atggattcga cagatcgcag
     1741 gatcgtggcc ctcctagaga aggacgcccg cctgacctac ggcgaggtcg gcaagcgggt
     1801 cggcctggcg gcgtcgtcgg tccacgaccg cgtccgcaag ctcgagaaga agggcgtcct
     1861 gcggggctac cgggcggacg tcgccttctc ctccgtcggg ctgccgatca cggctttcgt
     1921 gagcctcgcc ctccggccgt cgtcccctgc ggacgtcccg gcccgtgtgg cggagttccc
     1981 gctcgtcgag tcctgctact cggtcgccgg cgacaactcg tacgtgctgg tcgtccgggc
     2041 ccccaccacg agcgccctgg aggagctcct ggacgccctc agggccaagc tcgaggtcgt
     2101 cacccggtcg acgatcgtgc tgtccacccc cttcgaacgg aggccgatga cggccgaggg
     2161 gaattcttcg cggccctagc tgttatgttg gtccgagagc ggcaggtagt cgctcgtgag
     2221 attttcctct cgttcctcct cagagctaac gggttgcgag tctcccgtag tcgaaggagt
     2281 agagcttgac cacgttcacc gaactcggcg tgtccgcgcg cgtagtgcgg gcactggagc
     2341 accgaggcat cacacaaccg ttcgagattc aggcgctggt gatccgcgac gcgatcgcgg
     2401 gccgcgacgt cctggcgaag tcacgcaccg gctccggcaa gaccctcggc ttcggcatcc
     2461 ccgtcgtcga gcggctcgag cccgggccca agcgcccctc cgcgctgatc ctcgtcccga
     2521 cccgtgagct cgcattgcaa gtcgcgaccg aggtggaagc gatcgccacg gcacggagcc
     2581 ttcgggtcac cgccgtctac ggaggcgccg gcctacaggc gcaagcgcgc gccgccggcc
     2641 gggccgacat cctggtggcg acgcccggga ggctccagga cctgctcgac cggagagcca
     2701 tatcgctccg ttcgatccag atcctcgtcc tcgacgaggc cgaccggatg ctcgacatgg
     2761 ggttccagcc tcaggtcgac cgcatcctgc gcgacgcgcc gcagcagcgt cagacgatgc
     2821 tgttctccgc gacgctcgac ggcgtcgtcg gccgtctcgc gcgccagtac acgaaggacc
     2881 ccgcgacgca cgaggtgcac tcgtcgaagc cgaccgtcga agaggcggat catcgcttcg
     2941 tcacggcggc caaggaagca cgcgccgacg tgctggcgtc gttgctccgt gacgaggacg
     3001 gtgcgacgct cgtgttcgtc aggacgaagc gcggtgccga ccgtctcgcc aagcggctca
     3061 cccgggccgg gcacgacgcg gtcgcgatgc acggcgacct ctcgcagggt cagcgcgagc
     3121 gtgccctcgc caagttctcg tccggccggg cgcgcgtcct cgttgcgacg gacgtcgccg
     3181 cacgcggcct cgacgtcgac ctgatctcgc acgtcgtcaa cttcgacccc ccccacgacg
     3241 gtgacaccta cgtccaccgg gtcgggcgga cggcacgcgc cggtcgctcc ggtctcggca
     3301 ccacgctcgt gatgccggat caggccgccg agggggcgaa gtgggcgggc gagctgggcc
     3361 tccacgacgc gctcgccga
//
LOCUS       DASQDO010000180         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_752989, whole genome shotgun sequence.
ACCESSION   DASQDO010000180 DASQDO010000000
VERSION     DASQDO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752989"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VEV43_05270"
     CDS             complement(<1..355)
                     /locus_tag="VEV43_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP22963.1"
                     /translation="MTLDAGVSPSRSALKTLIAEDHTLVSQGLEVMLSMAEGVDLVGV
                     VATGEAAVERAEREQVDVILMDVNLGKAMNGIEATRLVKERAPSTRVLILTMFTDPGT
                     VAEAVKAGADGYLSKG"
     gene            complement(352..1722)
                     /locus_tag="VEV43_05275"
     CDS             complement(352..1722)
                     /locus_tag="VEV43_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYP22964.1"
                     /translation="MRQGKVFAWCLVVVTAAIFAWSLAETHGAGPAISTYIFWIALLA
                     AVELLPVSLGFGTEVTMSFPIYLALAILFRDQMWVPMLIAGIGSTDPREFTHEVPLWR
                     ALFNRAQSVLSVAAAGVILDAYGGSIFSFPTGTLVIAAAAIARLTLNFGLVVTMVRLD
                     IGQPFRDAVRALLPNPLAGFFISYVLLTGLGVVTAVAYEEIGAEAVAAILIPLFFARL
                     SIVGARTQQELSEKLSRQQEALLQATEKVFQERERERNRIAEDIHDSSLQMLAAAAYG
                     CGNAAELIDAGRTDQAQTTIANARDAIDGAIKELRGSLVDLRRSSVEEGGLMETIENY
                     VAHVSTLWGTEVRIEGGVAAEPPIPVSLAAVQILQEGLVNALKHAQGSTVVIRVEEAD
                     GFVHIVIEDDGTGFDPATSVDDDEHHGMRLMRERAERVGGRIELDTLPGRGTRVEAVL
                     PGGVAR"
     gene            2155..3162
                     /locus_tag="VEV43_05280"
     CDS             2155..3162
                     /locus_tag="VEV43_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYP22965.1"
                     /translation="MEAPEPVGPTVLIAERHLTVAESLARLVTESGAGRVAAKVQDPA
                     AALEMTRKIAPDVAIVALDLSPGCSLVRGLRDTCPDTRVIVLADRMDADDAQLLDALA
                     SGAVGAIYKESSSDDLARALSSSSSKTPVVVDEAAGLLLGSYVDAMTEKRLRDRATIE
                     ALAAAVEVRDAVTGQHLHRVTELATRCVDAIDSDLAKNEEVVFGFMLHDVGKIGVPDA
                     VLNKKGPLDAAEKEVMRRHPELGVKIVEPIGFSPMATDVIMCHHERWNGEGYPLGLRR
                     EEIPVVARAFAVADAFDAMTSDRPYRAALPAEDSLQVLKDAAYTEFDPDIVEVFVDLN
                     D"
BASE COUNT          530 a         1113 c         1076 g          541 t
ORIGIN      
        1 cgcccttcga caggtagccg tcggcgccgg ccttcaccgc ctcggccacc gttccggggt
       61 cggtgaacat cgtgaggatc agcacgcgcg tcgacggagc gcgctccttg acgagccgcg
      121 tcgcctcgat gccgttcatc gccttgccga ggttgacgtc catgaggatc acgtcgacct
      181 gctcgcgctc ggccctctcg acggcggctt cgccggtcgc caccacgccg acgaggtcga
      241 cgccctcggc catcgagagc atgacctcga ggccctgcga caccagcgtg tggtcctcgg
      301 cgatcagcgt cttgagcgcg gatcggctgg gcgatacgcc cgcatcgaga gtcatcgcgc
      361 taccccaccc gggagcacgg cctcgacgcg cgtgccgcgg cccggcaagg tgtccagctc
      421 gatgcgtcca ccgacgcgct ctgcccgctc tctcatcagc ctcatcccgt ggtgttcgtc
      481 gtcgtcgacc gaggtggcgg gatcgaaccc ggtcccgtcg tcctcgatga cgatgtgcac
      541 gaagccgtcg gcctcctcga cccggatcac gactgtactt ccctgcgcgt gcttcaacgc
      601 gtttacgagc ccttcctgga ggatctgcac cgcggcgagc gacacgggga tcgggggctc
      661 ggccgcgacg ccgccctcga tgcggacctc ggttccccac agcgtcgaga cgtgcgcgac
      721 gtagttctcg atcgtctcca tgaggccgcc ctcctcgacg ctcgaacgcc ggaggtcgac
      781 gagcgacccg cgcagctcct tgatcgcgcc gtcgatcgcg tcgcgggcgt tggcgatcgt
      841 ggtctgggcc tggtcggtgc ggccggcgtc gatgagctcg gcggcattgc cgcacccgta
      901 agccgcggcg gcgagcatct ggaggctcga gtcgtgtatg tcctcggcga tccgattccg
      961 ctcgcgctcg cgctcctgga agaccttctc cgtcgcttgg aggagggcct cctgctggcg
     1021 agagagcttt tctgagagct cctgctgggt ccgggccccg acgatggaga gccgtgcgaa
     1081 gaacaagggg atgaggatcg cagcgaccgc ctcggccccg atctcctcgt acgccacggc
     1141 ggtgacgaca ccgagcccgg tcagaaggac gtacgagatg aagaacccag ccaagggatt
     1201 gggcaacaac gcgcgtaccg cgtcccggaa cggctgaccg atgtccaggc ggaccatggt
     1261 cacgacgagc ccgaagttca acgtcaatcg agcaattgcc gcggccgcga tgacgagggt
     1321 tccggtcggg aacgaaaaga tgctcccgcc gtaggcgtcg aggatcaccc cggccgccgc
     1381 cacggacagc acgctttgcg cgcgattgaa gagcgctctc cacagaggga cctcgtgggt
     1441 gaactctcgc gggtccgtgg aaccgatgcc ggcgatgagc atggggaccc acatctggtc
     1501 ccggaacaag atcgcgaggg cgaggtaaat cgggaacgac atcgtgacct cggtcccgaa
     1561 cccgagcgat accgggagga gctcgacggc ggcgaggagc gcgatccaga agatgtacgt
     1621 cgagatcgcg ggccctgcac cgtgggtctc ggccagcgac caagcgaaga tcgcggcggt
     1681 gacgacgacg agacaccacg cgaagacctt cccctgcctc acgtctctat cgtaaggatc
     1741 acgggtcccg cgtcctgaat aagaaggaag ggagggcctc aggccctccc ttttcttcgt
     1801 tttcgcctta gtacttccag tgcgacgctc cggcggtctg gaacaacgcc gccagcacgc
     1861 cggcgaatgc aacaagcttg agtttcaaat gcggacctcc cctcacaaga aggtgtctgg
     1921 tcgaccggcc ctggggccgc cgctccgctc tcgctcacgc tttggccgat cgtcgagacg
     1981 atcggtcgct cacgctatgt cgcgcttgtc cgattcctcc gtttcttgcc ccttcgctga
     2041 cccgttcggg tacgcactcg acccgaacgg gggatacgtt gcccacgcta agggctgtta
     2101 actactggtg gcaatggcct gctcggccta tttcgtcatc ttttgaggga gttcttggaa
     2161 gctccagagc ccgttggccc cacggtcctc atcgcggaac ggcacctgac ggtcgccgag
     2221 tcgctggcgc ggctcgtcac cgaatccggt gccggccggg tcgctgcgaa ggtccaggac
     2281 cccgccgccg ccctcgagat gacccgcaag atcgcccccg acgtggcgat cgtcgccctc
     2341 gacctctcgc ccggatgcag cctcgtccgg gggctccgtg acacctgccc ggacacgcgg
     2401 gtgatcgtcc tcgcagaccg gatggacgcc gacgacgccc agcttctcga cgcgctcgct
     2461 tcgggggccg tcggcgcgat ctacaaggaa agctcgtcgg acgacctggc gcgcgccctc
     2521 tcgtcgtcgt cctcgaagac gccggtcgtc gtcgacgagg ccgcggggtt gttgctcggc
     2581 tcctacgtcg acgcgatgac ggagaagcgg ctgcgcgacc gggcgacgat cgaggccctg
     2641 gcggccgcgg tggaggtgcg tgacgcggtg accggccagc acctccatcg tgtgacggag
     2701 ctcgcgaccc gctgcgtcga cgcgatcgac tcggacctcg ccaagaacga ggaggtcgtc
     2761 ttcgggttca tgctccacga cgtcggaaag atcggggtgc ccgacgcggt gctcaacaag
     2821 aaggggccgc tcgacgcggc cgagaaggag gtcatgcggc gccatccgga gctcggcgtg
     2881 aagatcgtcg agccgatcgg gttctcgccg atggcgaccg acgtgatcat gtgccaccac
     2941 gaacggtgga acggcgaggg ctacccgctc ggcctgcgcc gcgaggagat cccggtggtc
     3001 gcgagggcct tcgccgtagc cgacgcgttc gacgcgatga cgtcggaccg cccctaccgg
     3061 gccgctctcc cggcggaaga ctcgctacag gtactgaagg acgcggcata cacggagttc
     3121 gaccccgaca ttgtcgaagt cttcgtcgac ctgaacgact aggccatccg cggcgtcgat
     3181 tcggtctgtc gatacgggcg ttcgctagag ttccgcccgt gcggactcaa gccaggtgga
     3241 tcgcctacgc ggccatcgcc
//
LOCUS       DASQDO010000181         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1119830, whole genome shotgun sequence.
ACCESSION   DASQDO010000181 DASQDO010000000
VERSION     DASQDO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119830"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="VEV43_05285"
     CDS             complement(<1..1758)
                     /locus_tag="VEV43_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYP22966.1"
                     /translation="MASSDALRTRRSFARLEHVLDVPNLIDIQKKSFDWLVDTESGGL
                     RETIDDISPIEDYTGNLAVQFGDFTFDDPVATIEECREKDLTYARPLTVTVGFINRET
                     GEIREQSVFMGDFPWMTERGTFIINGTERVVVTQLVRSPGAYLMEPKDAEKQVFVANL
                     MPARGSWLELEIDKKGKVFVRIDRKRKLPVTVLLRAMGYASDEEILNLFDNSLYIRNT
                     IESDTEVTKTEEGALIELFKKQRPGEPPSVDNARALLHQLFFDPKRYDLTRVGRYKLN
                     ARLHLDTDLDTRTLTHDDIIRLVAELVSLPKRLGMPEDIDGLEIKDYAAEAAIRPREP
                     IADHLDEYEHFGNRRLRTVGELIQEAFRIGLYRMERVVRERLTTEDADTITPQTIINI
                     RPVVAALKEFFGSSQLSQFMDQTNSLSGLTHRRRLSALGAGGLTRERAPIEVRDVHPT
                     HYGRMCPIETPEGPNIGLIGSLSSYAQVSEFGFVTTPYRVVENGVVTDNIVHLDANEE
                     DEKVIAQANAELDNNGRFVSDLVPCRSRDGEYALEPPSEVDYMDVSPEQIWSVTTALI
                     PFLEHDDANRALMGSNMQAQ"
     gene            complement(1987..>2265)
                     /locus_tag="VEV43_05290"
     CDS             complement(1987..>2265)
                     /locus_tag="VEV43_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22967.1"
                     /translation="RVPGRRGTKPRLLTVTISDARRRVVTKLRFRVRRGRTVKVRARG
                     AGEAGRYRYVIRRGDAGKALKRGSFTVKPGSTGAFKVPAGRTLVCRIA"
BASE COUNT          321 a          719 c          835 g          390 t
ORIGIN      
        1 ctgggcctgc atgttcgagc ccatcagcgc gcggttggcg tcgtcgtgct cgaggaacgg
       61 gatcagcgcg gtcgtgaccg accagatctg ctccggcgac acgtccatgt agtcgacttc
      121 cgacggcggc tcgagcgcgt actcgccgtc gcgcgagcgg cacgggacca ggtcggagac
      181 gaagcggccg ttgttgtcga gctcggcgtt cgcctgggcg atgaccttct cgtcctcctc
      241 gttcgcgtcg aggtggacga tgttgtcggt gaccacgccg ttctcgacga cccggtaagg
      301 cgtcgttacg aagccgaact ccgacacctg cgcgtagctc gacagcgagc cgatcaggcc
      361 gatgttcgga ccctccggcg tctcgatcgg gcacatgcgg ccgtagtggg tcgggtggac
      421 gtcgcggacc tcgatgggcg cgcgctcgcg ggtgagcccg cccgctccga gtgccgacag
      481 ccggcgacgg tgcgtcaggc ccgagagcga gttcgtctgg tccatgaact gcgacagctg
      541 cgaggagccg aagaactcct tgagcgccgc gacgaccggg cggatgttga tgatcgtctg
      601 cggggtgatc gtgtcggcgt cctcggtggt gaggcgctcg cgcacgacgc gctccatccg
      661 gtagaggccg atgcggaagg cctcctggat gagctcgccg acggtccgca ggcggcggtt
      721 gccgaagtgc tcgtactcgt cgaggtggtc ggcgatcggc tcgcgggggc ggatggccgc
      781 ctcggccgcg tagtccttga tctccagccc gtcgatgtcc tcgggcatgc cgaggcgctt
      841 gggcagcgac accagctcgg cgacgagccg gatgatgtcg tcgtgcgtga gcgtgcgcgt
      901 atcgaggtcg gtgtcgaggt gcagccgcgc gttgagcttg tagcggccga cccgcgtgag
      961 gtcgtagcgc ttgggatcga agaacagctg gtgcagcagg gcgcgcgcgt tgtcgaccga
     1021 cggcggctcg cccgggcgct gcttcttgaa cagctcgatc agcgcgccct cctcggtctt
     1081 ggtgacctcg gtgtcggact cgatcgtgtt gcggatgtag agcgagttgt cgaagaggtt
     1141 gaggatctcc tcgtccgacg cgtagcccat cgcgcgcagg agcaccgtga ccgggagctt
     1201 gcgcttgcgg tcgatgcgga cgaagacctt gcccttcttg tcgatctcga gctcgagcca
     1261 ggagccgcgg gccggcatca ggttcgcgac gaagacctgc ttctcggcgt ccttgggctc
     1321 catcaggtag gcgcccgggg agcggacgag ctgcgtgacg acgacgcgct cggtgccgtt
     1381 gatgatgaac gtgccgcgct cggtcatcca cgggaagtcg cccatgaaca ccgactgctc
     1441 gcggatctcg cccgtctcgc ggttgatgaa gccgaccgtg accgtgagcg gccgcgcgta
     1501 ggtcagatcc ttctcgcggc actcctcgat cgtggcgacc gggtcgtcga aggtgaagtc
     1561 gccgaactgg acggcgaggt tgcccgtgta gtcctcgatc ggcgagatgt cgtcgatcgt
     1621 ctccctcaag cccccgctct cggtgtcgac aagccagtcg aaggacttct tctggatgtc
     1681 gatcaggttc gggacgtcga gcacgtgctc gagacgcgcg aacgaacggc gggtgcgcag
     1741 ggcatcactg gaagccaaga cggcgactcc tcacttggcg gcggggattt tcttcgggcg
     1801 gaggtccgcg cgctctgccc tggagggctg aggcgcgacc gatcaatcta gcacgtgcgt
     1861 gtgcgtaccc ctcggagggg ctctcggcgc tcacggaccc cgcccggctc ggcccgtatg
     1921 tggcgggcga agcgtcgttg ggcggctgcg gctatcggcc gagcaaccag gagtgtacga
     1981 ggtcggtcaa gcgatgcggc agaccagcgt gcggccggcc gggaccttga aggcgccggt
     2041 cgatccgggc ttcaccgtga acgagccgcg cttgagcgcc ttgcccgcgt cgccgcggcg
     2101 gatcacgtag cggtagcggc cggcctcgcc ggcgccgcgc gcgcgcacct tgaccgtgcg
     2161 gccgcggcgc acgcggaagc gcagcttggt gaccacgcgg cgccgcgcgt cggagatggt
     2221 cacggtcagc aggcgcggct tggtcccgcg gcgcccgggc acgcg
//
LOCUS       DASQDO010000182         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_79962, whole genome shotgun sequence.
ACCESSION   DASQDO010000182 DASQDO010000000
VERSION     DASQDO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79962"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1514)
                     /locus_tag="VEV43_05295"
     CDS             complement(201..1514)
                     /locus_tag="VEV43_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HYP22968.1"
                     /translation="MDRHIVIEHRDALVYMLVEAAEIEHAICCQYLFAAFGLKQSTAE
                     GVTDRQLAAIERWRATILEIAAQEMLHFALVNNLLAALGAAPRVGRPNLPVQGRHYPP
                     GVQFALLPLGEPALRHFLYLERPEGISLVDAEGFESLREAEPVLDPEDIVPRPQDFST
                     VGELYRSIEEGFERLAERYGEEWVFIGDASSQATPETFQWPELVAVTDLKTAKQAIDV
                     IVEQGEGPRGHWKDAHYGRLLVVLGEYLTMKREDATFEPSRPVVAAHCRPPVDVESVC
                     LISDPLTSKVMDAFNVSYEILLYALARYFAHGHERQEQLDTLADVAVGLMIQVVRPLG
                     MALTELPLGEEWPDKTAGPAFELFHSSGYLLPHTWQAWVLLHERLTELEAFLLKTAAT
                     PGAPEVLTSVAAAVARLAGKLVEQMPRLAERPVGPAPSTLSGAGE"
     gene            complement(1524..2027)
                     /locus_tag="VEV43_05300"
     CDS             complement(1524..2027)
                     /locus_tag="VEV43_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22969.1"
                     /translation="MRLRGSTRAAAFVIAVAGFAGSMTGVAWAPKNLSTHVVEAACDV
                     KDQGAGAFLGSFTLTGFTTINDQLFGVGTASGTCTTRGGNVIVSGASLLVPVSVEELS
                     CHELDLLLGDTAIASSGMTVDTSGTHVYLSPGTRGAMARFCAAERLAATRPFAGMLTP
                     LSQLLFQ"
     gene            complement(2125..>2321)
                     /locus_tag="VEV43_05305"
     CDS             complement(2125..>2321)
                     /locus_tag="VEV43_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22970.1"
                     /translation="SLVRAYLPDWDQWLLSTNIVRVAERGADVAFDGVTAEAAALRLV
                     AYAVGLFLVALAVFDRREMA"
BASE COUNT          381 a          829 c          788 g          323 t
ORIGIN      
        1 agctgccgaa gcctccgcta ccgctccgcg tcgaccacaa gaacgccttc gattgaccct
       61 cctcggtgtc ccaggaaggg tccctccact tcaggagcca ccgaaggaag agaacgaagc
      121 cgaccacgcc tgcgccgaga atcccccaca cgaacatgcc gtcctcctga cgtcccctcc
      181 agagtacggc ggcggtgcga ttactcgccg gcccccgaga gcgtcgacgg cgccggaccg
      241 accggacgct cggccaggcg cggcatctgc tcgacgagct tgcccgcgag gcgcgctacc
      301 gcggccgcca cggacgtcag cacctcgggc gcaccggggg tggcagcggt cttcaggagg
      361 aacgcctcga gctcggtgag ccgctcgtga agcagcaccc atgcctgcca cgtgtggggc
      421 agcaggtagc cgctcgagtg gaacagctcg aaggccggcc ctgcggtctt gtccggccac
      481 tcctcgccca gcggcagctc ggtcagcgcc atccccaggg gccggacgac ctggatcatc
      541 agcccgacgg cgacgtccgc gagcgtgtcg agctgttcct ggcgctcgtg gccgtgggcg
      601 aagtagcgcg cgagcgcgta cagcaggatc tcgtacgaca cgttgaacgc gtccatgacc
      661 ttcgacgtca gcgggtcgga gatcaggcag accgactcga cgtcgaccgg cggccggcaa
      721 tgcgcggcga cgaccgggcg cgacggctcg aacgtcgcgt cctccctctt catcgtgagg
      781 tattcgccga gcacgacgag caggcgtccg tagtgcgcgt ccttccaatg cccccgcggc
      841 ccctcgccct gctcgacgat cacgtcgatc gcctgcttgg ccgtcttcag gtcggtgacc
      901 gcgacgagct ccggccactg gaacgtctcg ggcgtcgcct gcgacgacgc gtcgccgatg
      961 aagacccact cctcgccgta acgctcggcg agacgctcga aaccctcctc gatcgaccgg
     1021 tagagctcgc cgaccgtgct gaagtcctgc gggcgcggga cgatgtcctc gggatcgagg
     1081 accggctcgg cctcgcgcag cgactcgaac ccctccgcgt cgacgagcga gatcccctcg
     1141 ggccgttcta ggtacaggaa gtgacggagc gcgggctcgc ccagcgggag cagcgcgaac
     1201 tggactcccg gcgggtagtg gcggccctgg accggcaggt tcggccggcc cacgcgcggc
     1261 gcggccccca gagccgcgag caggttgttc acgagcgcga agtgcagcat ctcctgcgcc
     1321 gcgatctcga ggatcgtcgc gcgccaccgt tcgatggccg cgagctggcg gtcggtgacg
     1381 ccctctgccg tcgactgctt tagcccgaac gccgcgaaca ggtactgaca gcagatcgcg
     1441 tgctctatct ccgcggcctc gacgagcatg tagacgagcg cgtcacgatg ctcgatgacg
     1501 atgtgccggt ccacgccggc ggcctactgg aagagcagct ggctcagagg cgtgagcatg
     1561 ccggcgaagg gcctcgtcgc ggccagtcgc tccgcggcgc agaacctggc catggcgccg
     1621 cgcgtccccg gcgagaggta gacgtgcgtc cccgaggtgt ccaccgtcat gccggagctc
     1681 gcgatcgcgg tgtcgccgag caggaggtcg agctcgtggc aggacagctc ctcgaccgag
     1741 accgggacga ggaggctcgc cccgctcacg atgacgttcc cgccgcgggt cgtgcaggtg
     1801 cccgaagcag tcccgacgcc gaagagctgg tcgttgatcg tcgtgaaccc ggtcagcgtg
     1861 aaggagccca ggaacgcccc cgcgccctgg tccttgacgt cgcacgccgc ctcgacgacg
     1921 tgggtggaga ggttcttggg ggcccaggcg acacccgtca tggagccggc gaaacccgct
     1981 accgcgatga cgaacgccgc cgctcttgtc gatccgcgaa gcctcacaga agacaccctc
     2041 tctgacgtag aagaccctct ctgggacctt cctaccgggg cgaggcctgg aaataaatac
     2101 gtaagtccgc gggcgggggc cgcgtcaggc catctcgcgg cggtcgaaga ccgccagcgc
     2161 cacgaggaac agcccgaccg cgtaggccac cagccggagg gcggccgcct cggccgtcac
     2221 cccgtcgaac gccacgtcgg cgccgcgctc cgcgacgcgg acgatgttcg tgctgaggag
     2281 ccactggtcc cagtcgggca ggtaggcgcg gacgagcgac t
//
LOCUS       DASQDO010000183         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_631229, whole genome shotgun sequence.
ACCESSION   DASQDO010000183 DASQDO010000000
VERSION     DASQDO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631229"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="ruvX"
                     /locus_tag="VEV43_05310"
     CDS             complement(<1..327)
                     /gene="ruvX"
                     /locus_tag="VEV43_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYP22971.1"
                     /translation="MDPGERRVGLALADEETRFARPLEVVDVAATDPVERIGAVVAEH
                     GVTLVVVGKPVSLSGGDGVAVENAAPFVASLRDALPVDVVEHDERLTTVIAERGLRAG
                     GAGAAAR"
     gene            complement(336..>2903)
                     /gene="alaS"
                     /locus_tag="VEV43_05315"
     CDS             complement(336..>2903)
                     /gene="alaS"
                     /locus_tag="VEV43_05315"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYP22972.1"
                     /translation="VPSSSLVPNDPSLLLTNAGMNQFKPYFLGEQQSPWQRALSIQKC
                     FRTTDIDEVGKTVRHLTFFEMLGNFSFGDYYKEKACPWAWELVTESFGIDPELLWITI
                     YETDDEAHDIWRDAVGVRPERILRWDKKNNFWSMGVAGPCGPCSEIYVDLGERFGEPS
                     DQGPIGNDARYLEIWNLVFMQNDCNAAIEPVAELPRKNIDTGAGVERLAQFLQGAASI
                     YETDTIGTLVETASSLTGKSYGTDARTDRALRILADHGRSLTFLIADGVMPSNTERGY
                     VLRRVMRRAIREARLLGRSEPVLPSLIGTTIELMGVAYPDLVERRDFILEVAEREEER
                     FDATLRQGMGVLETEIGKARDLGAGLPGDAAFRLHDSFGFPVDLTVEIAGESGLEVDI
                     EAFEVLMAEQRSRARAARKTGEEAASVDAALALLEEHGGTEFIGNEHLRTDAKVIGIV
                     DDSEGVAVASEGDVVDLVLSRTTFYAEGGGQVGDRGRIRSSSGRADVLDTRRLVPGLT
                     GHRVKVSAGEIRVGDDVELDVDAEFRRGCERAHTATHILHWILRDRLGEHATQAGSLV
                     EPGRLRFDFNHFDALAGGQLEDVSGELQVRVVADDGVRAYETSFDYARSIGAMAIFGE
                     KYGDFVRVVEVGEYSKELCGGTHVAHTSQIGVVVVTGESSVGANLRRVEALVGHEGLR
                     FLEGKLAVLARAATVLKTNPDEVAERAERLVSSHKELERKLADAEQRSLEADAAGLAE
                     SAQELDGTRLVVARRDLGVDSLRKLAQQLKGRLGSAVVVLGASDGGKANLVAALTKDL
                     VSQGLSARELLGPGAGLLGGGAGGKPELSISGGPAADKIDEALEAVASAARESLAGR"
BASE COUNT          408 a         1098 c          953 g          444 t
ORIGIN      
        1 gcgcgccgcg gcccccgcgc ctccggcgcg cagaccgcgt tccgcgatca cggtcgtcaa
       61 tcgctcgtcg tgctcgacga cgtccacggg gagcgcgtcc cggagcgacg ctacgaaggg
      121 ggccgcgttc tcgaccgcca cgccgtcgcc gcccgacagg gacacgggct taccgacgac
      181 gaccagcgtc acgccgtgct ccgcgacgac cgcaccgatc cgctcgaccg ggtccgttgc
      241 ggcgacgtcg accacctcca gcggacgcgc gaagcgggtc tcctcgtccg ccaacgcgag
      301 cccgacgcgc cgctcccctg ggtccacgcc gaggatcatc gacccgctaa cgattccctt
      361 gccgcgctgg ccacggcttc gagcgcctcg tcgatcttgt ccgccgcagg ccctcccgag
      421 atcgagagct cgggcttgcc gcccgcgccc ccgccgagca ggccggcacc ggggcccagc
      481 agctcgcgcg cggacagccc ctgcgacacg aggtccttcg tcagcgcggc cacgaggttc
      541 gccttgcctc cgtccgacgc gccgagcacg accacggccg agccgagccg tccctttagc
      601 tgctgcgcga gcttgcgcaa ggaatcgacg ccgaggtccc gccgtgcgac gacgagccgc
      661 gtgccgtcga gctcctgcgc cgactccgcg aggcccgccg cgtcggcttc gagcgaccgc
      721 tgctcggcgt cggcgagctt gcgttcgagc tccttgtgcg acgacacgag gcgctcggcc
      781 cgctccgcga cctcgtcggg gttcgtcttg agcaccgtcg ccgcccgcgc gagcaccgcg
      841 agcttgccct cgaggaaacg aaggccctcg tggcccacca gcgcctcgac gcggcggagg
      901 ttcgcgccta cggagctctc gcccgtcacg actacgacgc cgatctggct cgtgtgcgcg
      961 acgtgcgttc cgccgcagag ctccttcgag tactcgccga cctcgacgac gcgcacgaag
     1021 tcgccgtact tctcgccgaa gatcgccatc gcgccgatcg agcgcgcgta gtcgaagctc
     1081 gtctcgtacg cgcgcacgcc gtcgtcggcg accacgcgga cctgcagctc gccggacacg
     1141 tcctcgagct gaccccccgc gagcgcgtcg aagtggttga agtcgaagcg cagacgcccg
     1201 ggctcgacga gcgatcccgc ctgcgtcgcg tgctctccca gccggtccct gaggatccaa
     1261 tggaggatgt gcgtcgcggt gtgggcgcgc tcgcacccgc gccggaactc cgcgtcgacg
     1321 tccagctcga cgtcgtcccc gacgcgtatc tcgcccgcgc tcaccttgac gcgatgaccg
     1381 gtgagacccg ggacgagccg gcgcgtgtcg agcacgtcgg cgcggcccga cgacgaccgg
     1441 atcctccccc ggtctccgac ctgccccccg ccttccgcgt agaacgtcgt tcggctgagc
     1501 acgaggtcga cgacgtctcc ttcgctggcg acggccacgc cctccgagtc gtccacgatc
     1561 ccgatcacct tcgcgtcggt gcgcaggtgc tcgttgccga tgaactcggt gccgccgtgc
     1621 tcctcgagca gcgcgagggc cgcgtcgacc gaagccgctt cctcaccggt cttgcgcgcc
     1681 gcgcgcgcac ggctccgctg ctcggccatc aacacctcga acgcctcgat gtcgacctcg
     1741 agcccggact cccccgcgat ctcgaccgtg aggtcgaccg ggaagccgaa gctgtcgtgc
     1801 agccggaacg ccgcgtcgcc gggaaggccg gcgccgaggt cgcgcgcctt gccgatctcg
     1861 gtctcgagca cgcccatccc ctggcgcagc gtcgcgtcga agcgttcctc ctcgcgttcc
     1921 gcgacctcga ggatgaagtc gcggcgctcg acgaggtcgg ggtaggcgac gcccatgagc
     1981 tcgatcgtgg tcccgatcag cgacggcagc accggctcgg agcgcccgag caggcgcgcc
     2041 tctcggatcg cacgccgcat gacgcggcgc agcacgtagc cgcgctcggt gttgctcggc
     2101 atcacgccgt ccgcaatcag gaacgtcagc gaccgtccgt ggtcggccag gatccgaagc
     2161 gccctgtcgg tgcgcgcgtc cgtcccgtac gacttgccgg tgaggctcga cgccgtctcg
     2221 acgagggtcc cgatcgtgtc ggtctcgtag atcgacgcgg ccccctgcag gaactgcgcg
     2281 agccgctcga ccccggcccc cgtgtcgatg ttcttgcgcg ggagctccgc gaccggctcg
     2341 atcgccgcgt tgcagtcgtt ctgcatgaag acgaggttcc agatctcgag gtagcgcgcg
     2401 tcgttgccga tcggcccctg gtccgacggc tcgccgaagc gctcgccgag gtcgacgtag
     2461 atctcggagc acggcccaca cggccccgcg acgcccatcg accagaagtt gttcttcttg
     2521 tcccacctga ggatgcgctc gggccggacg ccgacggcgt ccctccagat gtcgtgcgcc
     2581 tcgtcgtcgg tctcgtagat cgtgatccac agcagctcgg ggtcgatccc gaaggactcg
     2641 gtcacgagct cccacgccca ggggcacgcc ttctccttgt agtagtcgcc gaaagagaag
     2701 ttgccgagca tctcgaagaa cgtgaggtgg cggacggtct tgcccacctc gtcgatgtcg
     2761 gtggtgcgga agcacttctg gatgctgagc gcgcgctgcc acggcgactg ctgctcgccg
     2821 aggaaatacg gcttgaactg gttcatcccc gcgttcgtga gcagcagcga cgggtcgttg
     2881 gggactaggg acgaagacgg tac
//
LOCUS       DASQDO010000184         8909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_814610, whole genome shotgun sequence.
ACCESSION   DASQDO010000184 DASQDO010000000
VERSION     DASQDO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814610"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3036
                     /locus_tag="VEV43_05320"
     CDS             <1..3036
                     /locus_tag="VEV43_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HYP22973.1"
                     /translation="DYDDAHQLIMVEDPVGRKNHMFYDANGNVEKTRDALNEDTTMFY
                     DERNLLTKVIEPFDGTRSITTRYEYDEVGNLKKLISPRAHDAGQDSLTTKYVYDQIDQ
                     LKRIDLPYNTGDSSPASIYRKYDASGNVITTTVPVPTQDPALSEEQQWLAIPDDMKTQ
                     VTYFDTGLPRTIDDNVNALTTYDYNARGQQTQRKAGSELEEWSYYDDGMLALHYAPDE
                     AHVKYVYDENNNLKTATDGTGVERRTENAVKIEVTYDSVDRVAKVRHRKERGATEPFD
                     FTTYSYDLDGNVIERRENGVESADGTTVTTPAKRHTFTYDDAGWLQTQQDFLTTGCQK
                     IENTFTATGLESLRKVFKASQACDASPTFTVKQSSVWDYFKNGALKRLRTVNGNVDNT
                     TARDPIAPAVVIEKHEPGYLNTAGTVYFNGHKTSDSFFRKRSGTGHCDEAIDGCVAKY
                     TFDGRDRLIKEDRGHGAAGAVTDYTLNAAGSILTEITNGTTTNYTYDGLQLDYVTAGA
                     LTSNYIYDDEGKLECIVNSNSTTSCPTAQQANGLLKDYTWDGLNRLWDVESFKIETNG
                     SSTKTDDTTYTYDALDRVVEQTETHGIDTASPKDRKTTFSFLGLTSLVTKEVFEGASR
                     LSDNKTYTYDAYDTRISMVDDPKDAAPKTYTYGYDAHGSASTLLDDSDGTVKASYGYD
                     AYGGRDATLSSGDDENGRELDPLNPYRYTGRRTDTGSATLDMGARRFGPDSQTFLQPD
                     YYAGALANLGLSMDPLTGNRYALAAGNPLSYVEVDGHALQKSGPGGTSITDPDDEDLT
                     PGSSAHWRAFDEQQVADYKHEGGNRETLWDREVERWYGDNGLPVHAVPEECQTVPERQ
                     IGRTMSECFFHAIGSAPSGYSHGRTDRPSDSFAYEFAGRSWGQVQEDIKDVITGGGQV
                     CFWACVAVAFDSDGVSVTASGVSVGRGEGLNRGFGANVFLTSGEQAPGNGGAAQACVR
                     LCVGGYVNKSGTQGSLIGFGTFGFEVGPFIELLRFEFDE"
     gene            complement(3674..4225)
                     /gene="mug"
                     /locus_tag="VEV43_05325"
     CDS             complement(3674..4225)
                     /gene="mug"
                     /locus_tag="VEV43_05325"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HYP22974.1"
                     /translation="MARPTKEELAAAAGVTIRDVIAPGLDVLFCGINPGLYSGATGNH
                     FARPGNRFWPALHLSGFTPRQLHPSEKEELLSLGLGITNVVARTTARADELTDEEIVA
                     GGRALRRKVKRYGPRYLAVLGLGAYRTAFGTKTATVGPQPETIGDTKIWLLPNPSGLN
                     AHYQLPALAEEFARLRKAASVDS"
     gene            4241..4690
                     /locus_tag="VEV43_05330"
     CDS             4241..4690
                     /locus_tag="VEV43_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22975.1"
                     /translation="MARRTGACMNVDRLAGTDERYPWVRAVKASVVILCGVVPAIVLA
                     MMVVQSMTLDLFAGESVKYALILSAFFLAPAALYAALIRTKFPIVVVGLGLIVIEVWS
                     IWNVVTSTSSTGALAILTIPVAGIPFVLLGWAVDVWIRAMWPVSPQS"
     gene            complement(4674..5477)
                     /locus_tag="VEV43_05335"
     CDS             complement(4674..5477)
                     /locus_tag="VEV43_05335"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYP22976.1"
                     /translation="MPADDLSTDAIRAALRGSFGAELRTFDAIGSTNDEALAWAEAGA
                     PEGAVVTADQQTAGRGRWNRSWLSPPGRSISLSLVLRPASIEQAGLVTTLLGVAAAEA
                     VEAAAGIGCGIKWPNDVVAPGGKVAGILVESRSSGGSLGFVVAGIGVNVSWGPDEMPA
                     EIREGASSLVAEGAGPLPRAAVIAELLWAIEHWYGRCASASGRAEVVQAATQRSTILG
                     HPVLLRRSDGTLQEARAVALLEDGALQVEVDGSLVGVTAGEVERIRTEG"
     gene            5496..7232
                     /locus_tag="VEV43_05340"
     CDS             5496..7232
                     /locus_tag="VEV43_05340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HYP22977.1"
                     /translation="MIKKLLVANRGEIAVRLIRACRDLDVASVAVYSDLDVDAVHTRL
                     ADEAYNVGPGPASESYLKIPAILDAAKRAGADAVHPGYGFLAENGDFARAVVDAGLTW
                     VGPAGDVIDGMGDKTAARRAAADAGVPTVPGTKEPVTSAEEVRAFVAQNGLPIAIKAS
                     AGGGGKGFRVVSSEDAIEDALAGAAREAQAYFSSDDVYLERYLERPRHIEIQVLGDAS
                     GAIISFPERDCSLQRRHQKLVEESPSPALDQSVREAMMEAAARVSKQVGYRNAGTIEM
                     LLDSDGKTFYFLEMNTRLQVEHPVTELVTGVDLVKAQLLIASGEPLEFGQEDVQLRGA
                     AIECRINAENPAKGFLPAPGHIGAYREPQGPGVRVDSGAEADYTIPQAYDPLIAKLIT
                     SGATRDEARRRMLRALDEYVVEGIKTTIPFHSFMLADPRFVAGDYHTGTVEKETDLSV
                     LVDQSTSTVTDGARVAERSFGVELDGKRFDVRLREVLGDALPEKPRPPESAGSIGGGG
                     TEALAAPMQGTIVKVLVAQGDEVEAGQAVCVLEAMKMENSILATHAGTVEELRVEPGQ
                     SVETGATLAIIR"
     gene            complement(7242..7709)
                     /locus_tag="VEV43_05345"
     CDS             complement(7242..7709)
                     /locus_tag="VEV43_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22978.1"
                     /translation="MKMRSNRRVFFRIALCAALVSGALVTGGATALADCQEQIYVDAY
                     DVQLEPRRDAYRVGDTALIDGTVTRKDTGAPVSGVDFFVYIPPSRHGFVYGWTQTDSS
                     GNALLRMKLKRGEVATGATKIVGRAQEERADATCAQIIEYGELHLDRAFSIRR"
     gene            complement(7795..8184)
                     /locus_tag="VEV43_05350"
     CDS             complement(7795..8184)
                     /locus_tag="VEV43_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22979.1"
                     /translation="MSTDLRLAVANRPGTLARACDVLIEAGVAVEGMSGDLRPGEKWG
                     YVHVLVDDSAKAHEALSSAGIEVIGEHPVEIVDCTPGASLSEIVTRYTGERNMEVFYP
                     TLDGRLAIGTEDMRPDRPGVRMGDASY"
     gene            8289..>8909
                     /locus_tag="VEV43_05355"
     CDS             8289..>8909
                     /locus_tag="VEV43_05355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP22980.1"
                     /translation="MESTPDRSFQPLTTDLRIECCVIGGGIAGLTTALLLQRAGMKVA
                     VLEMDGIARGVSGYTTAKVSSLQGEVYQQLESKHGREAAALYAASNEAALAQVRSFVS
                     DLSIDCDLETRSNYTYCEDPSDLSKVEQEAEAASRAGLPVEFVHDTDLPFEVAGAVRL
                     DDQAQFHPRKYLLGLVDELERGGCLFFDETRATGLDDGDPCVVETSG"
BASE COUNT         1561 a         3040 c         2950 g         1358 t
ORIGIN      
        1 gactacgacg acgcgcacca gctgatcatg gtcgaggacc cggtcggccg gaagaaccac
       61 atgttctacg acgccaacgg gaacgtcgag aagacgcggg acgcgctcaa cgaggacacg
      121 acgatgttct acgacgagcg caacctgctc acgaaggtga tcgagccttt cgacggcacg
      181 cgctcgatca cgacccgata cgagtacgac gaggtcggga acctcaagaa gctgatcagc
      241 ccgcgggcgc acgatgcggg tcaggacagc ctcacgacga agtacgtcta cgaccagata
      301 gaccagctga agaggatcga cctcccctac aacaccgggg attcgagccc tgcgtcgatc
      361 taccgcaagt acgacgcgag cgggaacgtc atcacgacga cggtccccgt cccgacccag
      421 gacccggcac tgtcggagga acagcaatgg ctggcgatcc cggacgacat gaagacccag
      481 gttacgtact tcgacaccgg tctcccgcgc acgatcgacg acaacgtcaa cgcgctcacc
      541 acgtacgact acaacgcgcg cggccagcag acccagcgca aggccggcag cgagctggag
      601 gagtggagct actacgacga cggcatgctt gccctccact acgcgcccga cgaagcgcac
      661 gtcaagtacg tctacgacga gaacaacaac ctgaagacgg ccacggacgg gaccggggtc
      721 gagcggcgca cggagaacgc ggtcaagatc gaggtcacct acgacagcgt cgaccgcgtc
      781 gcgaaggtcc gccaccgcaa ggagcggggg gcgaccgagc cgttcgactt cacgacctac
      841 tcgtacgacc tcgacggcaa cgtcatcgag cgcagggaga acggcgtcga gagcgccgac
      901 gggacgacgg ttaccacccc ggcgaagcgc cacacgttca cgtacgacga cgccggatgg
      961 ctccagacgc agcaggactt cctgacgacg ggctgtcaga agatcgagaa cacgttcacg
     1021 gctaccggtc tcgagtcgct caggaaggtg ttcaaagcct cacaggcctg cgacgcgtcg
     1081 ccgacgttca ccgtcaagca gagcagcgtg tgggactact tcaagaacgg cgcgctcaag
     1141 cggctccgca cggtcaacgg caacgtcgac aacacgacgg cgcgcgaccc gatagctccg
     1201 gccgtggtga tcgagaagca cgagcccggc tacctgaata cggccgggac cgtgtacttc
     1261 aacggtcaca agaccagcga cagcttcttc cgcaagcgtt cggggaccgg acattgcgac
     1321 gaggccatcg acgggtgcgt cgcgaagtac accttcgacg gccgtgaccg gctcatcaag
     1381 gaagaccggg gccacggcgc ggccggggca gtcaccgact acacgctgaa cgcggcggga
     1441 agcatcctca cggagatcac gaacggcacg acgacgaact acacatacga cggcctgcag
     1501 ctcgactacg tgaccgccgg agccctcacg tccaactaca tctacgacga cgagggcaag
     1561 ctcgagtgca tcgtcaactc gaactcgacc acgtcgtgcc ccacggcgca gcaggcgaac
     1621 ggcctgctca aggactacac gtgggatggc ctcaaccggc tctgggacgt cgagagcttc
     1681 aagatcgaga ccaacggatc gtcgacgaag accgacgaca cgacctacac gtacgacgcc
     1741 ctcgaccgcg tcgtggagca gaccgagacc cacgggatcg acacggcgtc gcccaaggat
     1801 cgaaagacca cgttcagctt cctcgggctc acgagcttgg tcacgaagga ggtcttcgag
     1861 ggcgcttcga ggctctccga caacaagacc tacacgtacg acgcgtacga cacgaggatt
     1921 tcgatggtcg acgatccgaa ggacgcggcc cccaagacgt atacgtacgg ctacgacgcc
     1981 cacggctcgg cctcgacgct gctcgacgac tcggacggga ccgtgaaggc gagctacggc
     2041 tacgacgcct acgggggcag ggacgcgacg ctgtcgtcgg gcgacgacga gaacggccgc
     2101 gagctcgacc ccctgaaccc ctaccgctac accgggcggc gcacggacac gggctcggcg
     2161 acgctggaca tgggcgcccg ccgcttcggg ccggactcgc agacgttcct gcagccggat
     2221 tactacgcgg gggccctggc gaacctgggc ctgtcgatgg acccgctgac ggggaatcgc
     2281 tacgccttgg cggccggaaa cccgctgagc tatgtcgagg tcgacggtca cgcgctccag
     2341 aagtcggggc cgggcggcac ctcgatcacg gaccccgacg acgaggacct aacgcccgga
     2401 agctcagcgc actggagggc tttcgacgaa cagcaggtcg cggattacaa gcacgagggc
     2461 ggtaaccgcg aaacgctttg ggatcgtgag gtggagcgtt ggtatggcga caacggccta
     2521 cccgttcatg ccgtacccga ggagtgtcag acggtccccg agaggcagat cggccgcacg
     2581 atgtcggagt gcttcttcca cgccatcgga agcgcgccta gtggctactc acatggccga
     2641 accgaccgcc cttctgactc gttcgcgtac gagtttgcgg gccggtcatg gggccaagtc
     2701 caagaggaca tcaaagacgt gattacggga gggggacagg tctgcttctg ggcgtgtgtt
     2761 gcggtcgcct tcgactctga cggcgtgtcg gtgacggcct ctggcgtgag cgtcggccgc
     2821 ggggagggac tcaacagggg ttttggcgca aacgtcttct tgacttcagg tgagcaggct
     2881 cctggaaacg gcggggccgc ccaggcgtgt gtccgcctct gcgtcggcgg ctatgtgaac
     2941 aagtcgggaa ctcaaggttc gctgatcggg ttcggcactt tcgggttcga ggtcgggccc
     3001 tttatcgagc tcttgcgctt tgagttcgat gagtgacacc atgagaaaga tccgagcgga
     3061 tagtcggaac gagccccacg taggcgaaag cagcgatggg atggcaggag aacgcgagct
     3121 gttcacgtct cctcgggaag ctcgtctgta cctggcctcc ttttacttcc tccatgcaac
     3181 cgtgccattc gttttcccga cgtttctcta ctcgtcggcc gatcaggttt ggcccctcgt
     3241 cgtcttgatg tccgtgtacg ggccaccttt cgcatgcgcg gttcttggcg ttcgctacct
     3301 acccagacgc cgtaggtggt tgatcggtcg cgaatggccc gtcttctccg aagcgacgga
     3361 taaagcacga atcccgcgac gcccctccac ggcggcgttc tatgctggcc gagcagggct
     3421 gtggataacg ctcgtggtca tcctgctgcg agcgcgcgac ctggggttgg cctgagggtg
     3481 tcgtagcccg cccacgacgt gccggttcgt ttcgtcgtga acgtcggacc cgcagggcac
     3541 gctgggtggc tactcctgcc tcctgtcgac ggctgaagaa ctcctgagga tcccaaccca
     3601 aaggacacgt gggtagcgat ctagcgggtc ccgtcaaggg cgacgtattc gtcaggcggg
     3661 gctccccgag gagctacgaa tcaacggatg ctgccttgcg cagccgtgcg aactcttccg
     3721 ctagggccgg gagttggtag tgcgcgttca ggccgctcgg gttcggtagt agccatatct
     3781 tcgtgtcgcc gatcgtttcc ggttgcgggc ctaccgtcgc cgtcttcgtc ccgaaggccg
     3841 tcctgtacgc ccctagcccc agcaccgcca ggtaacgggg gccgtatctc ttcaccttcc
     3901 ggcggagggc gcggcccccg gccacgatct cttcgtccgt cagctcgtcg gccctcgccg
     3961 tggtccgggc tactacgttc gttatcccta ggcccagcga cagcagctcc tctttctccg
     4021 aggggtgtag ctgacgcggc gtgaagccgg acaggtgcag ggccggccag aaacggttgc
     4081 ccggccgcgc gaagtggttc cccgtcgctc ccgagtacag gcccgggttg ataccgcaga
     4141 acagcacgtc caggcccggc gcgatcacgt cgcggatcgt cacgcccgcc gccgcggcca
     4201 gctcttcctt cgtcggcctg gccattacgc tggggaggtc atggcccggc ggacgggggc
     4261 gtgcatgaac gtggacaggc tcgcgggcac ggacgaacgc tacccgtggg tgcgcgcggt
     4321 caaggcctcg gtggtcatcc tgtgcggcgt cgtgccggcg atcgtcctcg ccatgatggt
     4381 cgtgcagtcg atgacgctgg acttgttcgc cggagagtcc gtcaagtacg ccttgatcct
     4441 gagcgccttc ttcctcgcgc cggccgcctt gtacgcggcc ctcatccgta cgaagttccc
     4501 gatcgtcgtc gtcgggctcg gcctgatcgt catcgaggtg tggagcatct ggaacgtcgt
     4561 gacctcgacg agctctacgg gagccctcgc gatcctcacg atcccggtcg ccggcatccc
     4621 gttcgtgctc ctcggctggg ccgtcgacgt ctggatccgg gcgatgtggc cggtcagccc
     4681 tcagtcctga tccgctcgac ctcgcctgcc gtcacgccga cgagcgaccc gtcgacctct
     4741 acctgcagcg cgccgtcctc gagcagcgcg accgcccgcg cttcctgcag cgtcccgtcc
     4801 gaacgcctca gcaacaccgg gtggccgagg atcgtcgacc gctgagtcgc ggcctggacg
     4861 acctcggccc gaccggaagc gctcgcgcac ctcccgtacc agtgctcgat cgcccacagc
     4921 agctccgcta tcaccgccgc tcgcggaagc ggtccggccc cctccgcgac gaggctcgat
     4981 gccccctccc gtatctccgc aggcatctcg tccggccccc acgagacgtt cacgccgatc
     5041 cccgcgacga cgaaccccaa agagccgccg gacgagcgcg actccacgag gatccccgct
     5101 accttcccgc cgggcgcgac gacgtcgtta ggccacttga tcccgcaacc gatcccggcc
     5161 gcggcctcga cggcctctgc ggccgcgacc ccgagcaggg tcgtgaccag accggcctgc
     5221 tcgatcgagg ccgggcgcag caccagggac agagagatgg aacgccccgg cggcgagagc
     5281 cacgagcggt tccagcgccc ccgtcccgcg gtctgctgat cggccgtgac gaccgcgccc
     5341 tcgggagcgc cggcttcggc ccacgccaga gcctcgtcgt tcgtcgagcc gatcgcgtcg
     5401 aacgtgcgga gctccgcgcc gaacgaacct ctcagcgcgg cccggatcgc gtcggtgctg
     5461 aggtcgtccg cgggcacgca cttaggatac ggaccgtgat caagaagctg ctcgtcgcca
     5521 accgcggcga gatcgcggtg cggctcatcc gggcgtgccg cgacctcgac gtcgcgtcgg
     5581 tcgcggtcta ttccgacctc gacgtcgacg cggtccacac gcgcctcgcg gacgaggcgt
     5641 acaacgtggg gccgggcccg gcgtcggagt cgtacctgaa gatcccggcg atcctcgacg
     5701 cggcgaagcg agcgggggcc gacgcggtcc accccggcta cggcttcctc gcggagaacg
     5761 gcgacttcgc gcgcgccgtc gtggacgcgg gccttacgtg ggtcggcccg gcgggcgacg
     5821 tcatcgacgg catgggcgac aagaccgcgg cccgtcgcgc ggccgccgac gcgggcgtcc
     5881 ccaccgtccc cggcacgaag gagcccgtca cttccgcaga agaggtcagg gcgttcgtgg
     5941 cgcagaacgg gctgccgatc gcgatcaagg cctcggccgg aggcggcggg aaggggttcc
     6001 gggtcgtgtc gagcgaggac gcgatcgagg acgccctggc tggagcggcc cgcgaggcgc
     6061 aggcctactt ctcgtccgac gacgtctacc tcgagcgcta ccttgagcgt ccccgtcaca
     6121 tcgagatcca ggtcctcggc gacgcgtcgg gcgccatcat ctcgttccca gagcgcgact
     6181 gctcgctgca gaggcgtcac cagaagctcg tcgaggagtc gccgtcgccg gcgctcgacc
     6241 agtccgtgcg cgaggcgatg atggaagcgg cggcccgggt cagcaagcag gtcggctacc
     6301 gcaacgcggg gacgatcgag atgctgctcg actccgacgg caagaccttc tacttcctcg
     6361 agatgaacac gcgcctccag gtcgagcacc cggtgaccga gctcgtcacc ggcgtcgacc
     6421 tcgtcaaggc ccagctgctg atcgcgtcgg gcgagccctt ggagttcggc caggaggacg
     6481 tccagctacg cggcgcggcg atcgagtgcc gcatcaacgc ggagaacccg gcgaagggat
     6541 tcctgccggc ccccggtcac atcggcgcgt accgggagcc gcaggggccg ggcgtgcgcg
     6601 tggactcggg ggccgaggcc gactacacga tcccgcaggc ctacgacccg ctgatcgcga
     6661 agctcataac gagcggtgcg acgcgcgacg aggcgcgccg ccgcatgctg cgtgccctcg
     6721 acgaatacgt cgtcgaaggg atcaaaacga cgatcccgtt ccactcgttc atgctcgccg
     6781 acccgcgctt cgtcgcgggc gactaccaca cgggcacggt cgagaaggag acggacctgt
     6841 ccgtgctcgt cgaccagtcg accagcacgg ttacggacgg tgcccgcgtc gcggagcgca
     6901 gcttcggcgt cgagctcgac gggaagcgct tcgacgtgcg gctgcgcgag gtgctcggcg
     6961 acgcgctccc ggagaagccg cggccccctg agagcgccgg ttcgatcggc gggggcggga
     7021 ccgaggctct cgcagcgccg atgcagggga cgatcgtcaa ggtcctcgtc gcgcagggtg
     7081 acgaggtcga agccggccag gccgtctgcg tcctcgaggc gatgaagatg gagaactcga
     7141 tcctcgcgac gcacgcggga acggtggagg agctgagggt cgagccgggt cagtccgtcg
     7201 agacgggcgc gaccctcgcg atcatccgct agagagcggc gctacctccg gatcgagaac
     7261 gcgcggtcca ggtgcagctc gccgtactcg atgatctgcg cgcaggtggc gtccgcccgc
     7321 tcctcctggg ccctcccgac gatcttcgtc gctccggtcg cgacctcgcc acgcttcagc
     7381 ttcatccgca gcagcgcgtt gccggacgag tcggtctggg tccacccgta cacgaacccg
     7441 tggcgagacg gcgggatgta gacgaagaag tcgacgcctg acaccggagc gcccgtgtcc
     7501 ttgcgggtga ccgtgccgtc gatcagcgcg gtgtcgccga cgcgataggc gtcgcgccgc
     7561 ggctcgagct ggacgtcgta ggcgtcgacg tagatctgtt cctggcaatc cgcgagggcg
     7621 gtcgcccccc cggtgacgag cgcgcccgag accagggccg cgcacagagc gatacggaag
     7681 aacacacgac ggttggaacg catcttcatg acctcacctg cccgctaggt ggatatgtcg
     7741 ctttcctgcc ccttccgcac gtaatccacc cgccgcggta acggccagaa ggggtcaata
     7801 gctggcgtcg cccatcctga cccccgggcg gtccggccgc atgtcctccg tcccgatcgc
     7861 cagccggccg tcgagcgtcg ggtagaagac ctccatgttc cgctcgcccg tgtagcgcgt
     7921 gacgatctcg ctgaggctcg cgccgggcgt gcagtcgacg atctcgaccg gatgctcgcc
     7981 gatcacctcg atgccggcgg acgagagggc ctcgtgcgcc ttcgccgagt cgtctaccag
     8041 gacgtgcacg tagccccact tctcgccggg gcgcaggtcc ccggacatcc cctccaccgc
     8101 cacgcccgcc tcgatgagga cgtcgcacgc cctcgcgagc gtgccggggc ggttcgccac
     8161 tgccagccgc agatccgtgc tcacgggcca cctccggttt gatcggctat tcccgaagac
     8221 tccgtatcct cgccgcgtcc gtcaaatcga ggggagcgag catggccggt cacaaccttt
     8281 cactctgggt ggagtccaca cccgacaggt cgttccagcc cctgacgacg gacctgcgca
     8341 tcgaatgctg cgtgatcggc ggcgggatcg cgggcctcac taccgccctg cttctccagc
     8401 gcgccgggat gaaggtagcg gtgctcgaga tggacgggat cgccaggggc gtaagcggat
     8461 acacgacggc gaaggtgagc tcgttgcagg gcgaggtcta ccagcagcta gagagcaagc
     8521 acgggcgcga ggcggccgcg ctctacgcgg cgtcgaacga agccgcgctc gcgcaggtgc
     8581 gctcgttcgt cagtgacctc tcgatcgact gcgatctcga gacgaggtcg aactacacgt
     8641 actgcgaaga cccgtcggac ctctcgaagg tcgagcaaga ggccgaagcg gcgtcgaggg
     8701 ccgggctccc cgtcgaattc gtccacgaca ccgacctccc gtttgaggtc gccggcgcgg
     8761 tacggctcga cgaccaggcc cagttccatc cgcgcaagta cctgctgggg ctcgtcgacg
     8821 agctcgagcg cggcggctgc ctcttcttcg acgagacgag ggcgaccggc ctcgacgacg
     8881 gggacccgtg cgtcgtcgag acgagcggc
//
LOCUS       DASQDO010000185         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1120639, whole genome shotgun sequence.
ACCESSION   DASQDO010000185 DASQDO010000000
VERSION     DASQDO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120639"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VEV43_05360"
     CDS             <1..280
                     /locus_tag="VEV43_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYP22981.1"
                     /translation="PGVIAVTGRGTRPCRNAGVEKPRRYTQKELQKRLEAAGWVKTTG
                     GKHVVKMVKPGRRAITIPICRGETLSVGLCRRILKQAGIDGRSHNGKH"
     gene            312..551
                     /locus_tag="VEV43_05365"
     CDS             312..551
                     /locus_tag="VEV43_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYP22982.1"
                     /translation="MDKQYEIQVLIHEDETGYWATVAELPGVFAAGEERQQLLECLDE
                     AIRLYLDDTGIPPELEGPPILVEKFNVTERGLVLA"
     gene            complement(719..877)
                     /locus_tag="VEV43_05370"
     CDS             complement(719..877)
                     /locus_tag="VEV43_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22983.1"
                     /translation="MKKVRLLASAAIAVGCLAVFAPAASAGEVCYSIHVAAQGGDVVN
                     ETACIPLP"
     gene            985..2139
                     /locus_tag="VEV43_05375"
     CDS             985..2139
                     /locus_tag="VEV43_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP22984.1"
                     /translation="MSVEPRPPAAIGDYQLATIGQLVERVAPRFDDIVETMMTAYTTR
                     IPSYAAASPELMEDFRQGAKASVFAGLAILQGSVSTESLGNPLADIGRRRARQGLPLQ
                     DVLLAFQIGARTVWDTLLDEVPDDATQRAEIVEALMVALLDLTQAATTTVSAAYVEES
                     EALLADEELDLQVIVEVLSGTRSYDANLAERAERRDIDLSSISWCVARCVPEDESGQW
                     VRDLRRAMPTAAVGRYADAVVAFVDGEGPPDVPEASELGVAGAADTTEGSRRARAAAK
                     VAAFFGQDVVRYDEVVPLAAVLDAPDEDRAAFVEAHLGALLRDPRGEDLVASLDAYYR
                     HGSIATAARALFVHRHTLEYRLKRIEDLVKTDLSDPMTRLLMQLALVLRR"
     gene            complement(2136..3278)
                     /locus_tag="VEV43_05380"
     CDS             complement(2136..3278)
                     /locus_tag="VEV43_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HYP22985.1"
                     /translation="MSEPWGDIPLFREIQRILASSKGPVNVEIATQVATALATQEGDA
                     RVDAARVRALAEAVRSAELVLAGYTRLPVDDPAGVRGVGRAEWAKETLASWGWLFEHV
                     AQKLTGGMGPEHGDEGDAPPGIAAAMAQVAPLLVGIQVGTLVGRLARDALGRYDHAIP
                     RDDDGRLLFVDPNVARVATEYDLDEAAFVRWLALGAAARHLVLRQAPWVSRYWRSRLV
                     ADVDALEIDVGELQTRMMDLQSQGPSALEDGMGAGAALPLVPTDRHRRALARLRSFLA
                     AVEGYARHSSAALGAAIVGPAGAIEEGVARHRLAGNEAEAMLKTILGVSFDRDLEAAG
                     ATFCAAVVDRKGIAALNVMWAAPDNLPSYEEIKDPFAWMERVLEAG"
     gene            3312..4364
                     /locus_tag="VEV43_05385"
     CDS             3312..4364
                     /locus_tag="VEV43_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP22986.1"
                     /translation="MTTVAITGSSGYLGARLVELLAADESVTRIVGFDVRPPKAPHPK
                     LVFDEVDVRSPALAARLEGVDCLFHLAFVMDPIRDETRMRDVNVNGSQNVFKTAGRAG
                     VGRIVYTSSAVVYGAHPDNDVPLTEDSPLRANLDFSYAAHKLEVEYVLRELADDLSDA
                     HVVVFRPAVVGGPDADNAWSHLVEQPLLFGVAGHTPPLQLVHEEDVARALHWALTTDA
                     SGTFNLAAEGWLEASDALALADRRVRYLPEPLAFTVAEKAWRLGLSEVPAGYLHYAMH
                     PWVVGTEKLTAAGFTCERGHAETLRETVAAAGGRVRIGSRRFERADLVKRAAAGLGLV
                     GAAGAALAVRSRRRTA"
BASE COUNT          677 a         1720 c         1579 g          648 t
ORIGIN      
        1 tcccggtgtg atcgccgtca cagggcgcgg cacgcgtccg tgccgcaatg ccggtgtgga
       61 gaagccgcgc cggtacacgc agaaggagct gcagaagcgc ctcgaagccg cggggtgggt
      121 gaagaccacc ggcggcaagc acgtcgtcaa gatggtcaag ccgggccgtc gcgctatcac
      181 catcccgatc tgccgggggg agaccctttc ggtcgggctt tgccgcagga ttctgaaaca
      241 ggccgggatt gacggccgga gtcacaacgg aaagcattga gggccgaacg gtcgaggtcg
      301 gaggcgcccg catggacaag cagtacgaga tccaagtcct gatccacgaa gacgagaccg
      361 gctactgggc caccgtcgcc gagctgcccg gcgtcttcgc cgccggtgag gaacgtcaac
      421 agctcctgga gtgcctcgac gaggcgatcc ggctgtatct cgacgacacg gggatccctc
      481 ccgagctgga aggcccgccg atcctcgtcg agaagttcaa cgtcaccgag cgcgggctcg
      541 tcctggcgta accccgggcg cgagcgaggc tcccccgtac ccgtggggag cctcgtcgct
      601 tcttgcgcgg ccctggggac gcctggcggc gaaccgcgca ttcagggggt gcaggccacc
      661 cacccggcct ggggattgcc tggcggcgaa ccgggcgggc ggcctgcgtg tcagacggtt
      721 acggcagggg gatgcaggcg gtctcgttga cgacgtcgcc gccctgggcg gccacgtgga
      781 tcgagtagca gacctcgccg gccgaagcgg cgggggcgaa cactgcgaga caaccgaccg
      841 cgatcgcagc cgaagcgagc agacgcacct tcttcattcc aagtccctcc ctctaggcga
      901 caccgttacg gggcagggtt ctgccacccg agaaagtccc ctttagacgg attgcgccag
      961 aactcgcgct tagtcttgga caacatgtcg gttgaacccc gcccgcccgc tgctataggc
     1021 gactaccagc tggctacgat tggccaactc gtagagcggg tcgcgccgcg cttcgacgac
     1081 atcgtcgaga cgatgatgac ggcctacacg acccgcatcc cgagctacgc cgccgcaagc
     1141 cccgagctca tggaggactt ccgccagggc gccaaggcca gcgtcttcgc cgggctcgcg
     1201 atcctccagg gctccgtatc gacggagtcg ctcgggaacc ccctcgcgga catcggccgc
     1261 cgccgtgccc gccaggggct cccgctccag gacgtgctgc tcgccttcca gatcggcgcg
     1321 cggacggtct gggacacgct gctcgacgag gtgccggacg acgccaccca gcgcgccgag
     1381 atcgtcgagg cgctgatggt cgcgctcctc gacctcacgc aggcggcgac gaccacggtc
     1441 tccgccgcgt acgtcgagga gtccgaggcg ctgctcgccg acgaggagct ggacctgcag
     1501 gtgatcgtcg aggtcctgtc ggggacccgg tcgtacgacg cgaacctcgc cgagcgggcc
     1561 gagcggcgcg acatcgacct ctcgtcgatc tcgtggtgcg tcgcccgctg tgtcccggag
     1621 gacgagtcgg ggcagtgggt gcgcgacctg cggcgagcga tgcctacggc ggccgtcggc
     1681 cgctacgcgg acgccgtggt cgcgttcgtc gacggcgagg gccccccgga cgtccccgag
     1741 gcctccgagc tcggcgtcgc aggggccgcc gacacgaccg agggctcacg ccgggcccgc
     1801 gccgccgcga aggtcgcggc cttcttcggg caggacgtcg tccgttacga cgaggtcgtc
     1861 ccgctggccg cggtcctcga cgcgcccgac gaagaccgcg ccgcgttcgt cgaagcccat
     1921 ctcggcgcgc tcctgcgcga cccccgcgga gaagacctcg tcgcgtcgct cgacgcgtat
     1981 taccgccacg gctcgatcgc gaccgccgcc cgcgctctgt tcgtccaccg ccacacgctc
     2041 gagtaccggc tcaagcggat cgaagacctc gtgaagaccg acctgtccga cccgatgacg
     2101 cggctgctca tgcagctcgc gctcgtgctc cgccgctagc cggcctccaa gaccctttcc
     2161 atccaggcga agggatcctt gatctcctcg tacgagggga ggttgtccgg agcggcccac
     2221 atgacgttca gcgccgcgat ccctttccgg tcgacgaccg ccgcgcagaa cgtcgccccc
     2281 gcagcttcca gatcgcggtc gaacgagacc ccgaggatcg tcttgagcat cgcctcggcc
     2341 tcgttgcccg cgagccggtg acgcgcgacc ccttcctcga tcgcgccggc cggcccgacg
     2401 atcgcggccc ccagcgcagc gctcgagtgc cgcgcgtagc cctccaccgc cgcgaggaac
     2461 gagcgcagtc gcgccagcgc gcgccggtgc ctgtcggtcg gcacgagcgg cagcgcggcc
     2521 cccgcgccca tcccgtcctc gagcgcgctc ggcccctgcg actgcaggtc catcatccgc
     2581 gtctgcagct cgcccacgtc gatctccagc gcgtcgacgt ccgcgacgag acggctgcgc
     2641 cagtaccggc tgacccacgg cgcctggcgg agcacgaggt ggcgcgctgc ggcccccagc
     2701 gcgagccagc ggacgaacgc ggcctcgtcg aggtcgtact cggtggcgac gcgggcgacg
     2761 ttggggtcca cgaagagcag gcggccgtcg tcgtcgcgcg ggatcgcgtg gtcgtagcgc
     2821 ccgagggcgt cccgcgcgag ccggccgacg agagtcccga cctggatccc gacgaggagc
     2881 ggcgccacct gcgccatcgc ggccgcgatc ccgggcggag cgtccccttc gtcgccgtgc
     2941 tccggcccca tgccgcccgt cagcttctgc gcgacgtgct cgaagagcca tccccacgaa
     3001 gccagcgtct ccttcgccca ttccgcgcgc ccgacgcctc tgacccccgc gggatcgtcg
     3061 acggggaggc gcgtgtaccc ggcgagcacc agctcggccg agcgcaccgc ctcggccaga
     3121 gcgcggacgc gcgccgcgtc cacccgcgcg tcgccctcct gggtcgccag cgcggtcgcc
     3181 acctgcgtcg cgatctcgac gttcacgggg cccttgctcg aagcgaggat ccgctggatc
     3241 tcgcggaaca acgggatgtc gccccagggc tcgctcatcg ccgcccattg tcgcccggta
     3301 cgctcgcaac gatgacgacc gtcgcgatca ccggctcgtc cggatacctg ggggcccggc
     3361 tcgtcgagct cctcgccgcc gacgagtcgg tcacgcggat cgtcggcttc gacgtccgcc
     3421 ccccgaaggc gccgcatccc aagctcgtct tcgacgaggt cgacgtgcgc agccctgcgc
     3481 tcgcggcccg gctcgaaggc gtcgactgcc tcttccacct cgcgttcgtg atggatccga
     3541 ttcgtgacga gacgaggatg cgcgacgtca acgtgaacgg cagccagaac gtcttcaaga
     3601 ccgcgggccg cgccggagtc gggcggatcg tctacacgtc ttccgcggtc gtgtacggcg
     3661 cgcaccccga caacgacgtc ccgctcaccg aggactcgcc gctgcgcgcg aacctggact
     3721 tcagctacgc ggcgcacaag ctcgaggtcg agtacgtcct ccgcgagctc gccgacgatc
     3781 tctccgacgc gcacgtcgtc gtgttccgcc ccgcggtcgt cgggggtccc gacgcggaca
     3841 acgcgtggtc gcacctcgtc gagcaaccgc tgctgttcgg ggtggccggc cacacgccgc
     3901 cgctccagct cgtgcacgaa gaagacgtcg cgcgtgcgct gcactgggcc ctgaccacgg
     3961 acgcgagcgg gacgttcaac ctcgccgccg agggatggct cgaagcgagc gacgcgctcg
     4021 ccctcgcaga ccgccgcgtc cgctacctgc cggagccgct ggcgttcacc gtcgccgaga
     4081 aggcgtggcg tctcggcctg tccgaggtcc ccgccggcta cctccactac gcgatgcacc
     4141 cctgggtcgt cgggacggag aagctcacgg ccgcgggctt cacctgcgag cggggccacg
     4201 ccgagaccct gcgcgagact gtcgccgccg cgggcgggcg ggtccggatc gggtcccggc
     4261 gcttcgaacg ggccgacctg gtcaagcgcg ccgccgccgg cctgggcctc gtgggggcgg
     4321 ccggggccgc cctggcggtc cggtcccgcc ggagaacggc ctgatagcct ggcagggagg
     4381 tccccgcggg cgcagaacaa tcaacgggga ccacgtgcca cgccccggcg cgccgtccgt
     4441 gaccatttcc ggacaacgcg ggatgccgcc ggtgtgtagc gcggctccgt ccgcttctcg
     4501 actctccgaa atggaggacg cgtgatctac ctggtcatca tgttcctggt cgcaggcacc
     4561 gggctcgcca gcctgtgggt gcagcagcga cggacgaggg cacacctcga caccgtcgac
     4621 ggct
//
LOCUS       DASQDO010000186         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_386560, whole genome shotgun sequence.
ACCESSION   DASQDO010000186 DASQDO010000000
VERSION     DASQDO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386560"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VEV43_05390"
     CDS             complement(<1..188)
                     /locus_tag="VEV43_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22987.1"
                     /translation="MYTVLRAGRGRKEDGVATPVVAAAAAAVLVVLTASAVLFSMTRE
                     DNASVASKSAPEQAGPEA"
     gene            complement(240..1265)
                     /locus_tag="VEV43_05395"
     CDS             complement(240..1265)
                     /locus_tag="VEV43_05395"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYP22988.1"
                     /translation="MRAVLVGATGAVGGEILKILSERPPFLDELIPVASPRSAGRKLP
                     YRDGEVEVRALAPEVFDGVDLALFDVPDEIAVEWAPVAAERGCVVVDNSAAWRMDADV
                     PLVVPEINPGAIANRPKGIIASPNCTTLAMVVPIAALHAEVPLRRLVLASYQAASGSG
                     RPGTDELWEQTQIAAKEPDAVQEGRARDVLERGETFTHPIAMNVIPQCGSLKEDGYTS
                     EELKLCRETRKIMDLPDLRVTATCVRVPVVVGHGVAVHAEFEGPITPERARELLQGAP
                     GVELRDDPANAVYPTPLEAAGEDPCYVGRIRQDLFDPNALELFCVADNLRKGAALNTV
                     QVAELLA"
     gene            complement(1262..2521)
                     /locus_tag="VEV43_05400"
     CDS             complement(1262..2521)
                     /locus_tag="VEV43_05400"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HYP22989.1"
                     /translation="MATIVQKYGGTSVGDAHRIKKVAERVVSCAEAGHRVCVVVSAMG
                     HSTDELLDLAAEVSTVPHPRELDMLLTAGERISMALLSMAINEQGRGAISFTGSQAGI
                     VTDTTHGKARILEVKAQRVREALDAGSIAIVAGFQGVSTAFDVTTLGRGGSDTTAVAL
                     AAALDADYCEIYTDVAGVFTADPRLVPSARKLHAVSYEEMLELSASGAKVLMLRSVEY
                     ARNYQVMLHVRSSFTDDEGTWVREEDERMERAIISGIAHDTSEAKVTILGVPDKPGIA
                     ARVFRPLADEGVNVDMIVQNASAEGRTDIFFTLPKDDLVRAEPILKSIEDEVGAEGVT
                     TDPDIAKVSLVGAGMKTHPGVAADMFDALADAGINIEIISTSSIRVSCVIRASEVDRA
                     VKVIHDRFDLSRESLLREHPDDQPGAA"
     gene            complement(2548..>3236)
                     /locus_tag="VEV43_05405"
     CDS             complement(2548..>3236)
                     /locus_tag="VEV43_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22990.1"
                     /translation="GRDKAVDVLWAEAGIQFDPMVVEAFVAALPTRATVAGLMALFAA
                     PSWLFRKAAVQAKRLGVGNVTPALGALGAAALMGGFIPGAGPADGPKIAIERADDPQE
                     SDVVDLSTQTDDLEPVEDVADSKPATKPKPVAQKEKSGPDTVVLGSRLTRGARPKTTP
                     SGTNTATPPTSPPARTGQPPRPAQPTSKPKPTPDPQDDEHVQGDPQPGRGRDCEVPKD
                     DSKGNSKHCG"
BASE COUNT          432 a         1149 c         1143 g          512 t
ORIGIN      
        1 ctcgcctccg ggccggcctg ctcgggcgcg ctcttcgacg cgaccgacgc gttgtcttcg
       61 cgagtcatcg agaagagcac cgccgaagcg gtgagaacca cgagaacggc cgccgcggcg
      121 gcggcgacta ccggggttgc gaccccgtct tccttgcgac cccgtcctgc acgaagcacc
      181 gtgtacatcc ggcatccccc ctttgggggt tcttcgggga ccagcgtgcc cggcttgagc
      241 taggcgagga gctcggccac ctgaacggtg ttgagcgcgg cccccttgcg gaggttgtcg
      301 gcgacacaga acagctctag cgcgttcggg tcgaagaggt cctggcggat gcggccgacg
      361 tagcaggggt cctcgcccgc ggcctccagc ggcgtcgggt agacggcgtt cgccgggtcg
      421 tcgcggagct cgacgcccgg ggcgccctgc agcagctcgc gagctcgctc gggcgtgatc
      481 ggtccctcga actccgcgtg gaccgcgaca ccgtggccga cgacgaccgg cacgcggaca
      541 cacgtcgcgg tgacgcgcag gtcgggcagg tccatgatct tgcgcgtctc gcggcagagc
      601 ttcagctcct cgctcgtgta gccgtcctcc ttgagcgagc cgcactgcgg gatcacgttc
      661 atcgcgatcg ggtgggtgaa cgtctcgccg cgctcgagca cgtcgcgggc gcggccctcc
      721 tgcacggcgt cgggctcctt cgcggcgatc tgcgtctgct cccagagctc gtcggtgccg
      781 gggcggcccg atcccgacgc ggcctgatac gaggcgagca cgagccggcg cagcgggacc
      841 tccgcgtgca gcgccgcgat cggcacgacc atagcgagcg tcgtgcagtt ggggctcgcg
      901 atgatcccct tgggacgatt tgcgatcgca ccggggttga tctccggcac gacgagcggg
      961 acgtcggcgt ccatccgcca cgcggcggag ttgtcgacga cgacgcagcc gcgctcggcc
     1021 gcgacggggg cccactcgac cgcgatctcg tcgggcacgt cgaacagcgc gaggtcgacg
     1081 ccgtcgaaga cctccggtgc cagcgcgcgc acctcgacct cgccgtcgcg atacgggagc
     1141 ttgcggcccg ccgaacgggg cgacgcgacc gggatcagct cgtccaaaaa cggcgggcgt
     1201 tcggacagga tcttcaggat ctctccgccc acggcgccgg tcgcgcctac gaggacggcc
     1261 ctcatgcggc acccggctgg tcgtcgggat gctcgcgcag cagcgactcg cgcgacaggt
     1321 cgaagcggtc gtggatgacc ttcaccgcac ggtcgacctc ggaggcccgg atcacgcacg
     1381 agacgcggat cgaagacgtc gagatgatct cgatgttgat gcccgcgtct gcgagcgcgt
     1441 cgaacatgtc ggcggccaca ccggggtgcg tcttcatccc ggcccccacg agcgagacct
     1501 tcgcgatgtc ggggtcggtc gtgacgccct ccgccccgac ctcgtcctcg atcgacttca
     1561 ggatcggctc ggcacggacg aggtcgtctt tcggcagcgt gaagaagatg tccgtgcggc
     1621 cttccgccga cgcgttctgc acgatcatgt cgacgttcac gccctcgtcc gcgagcgggc
     1681 ggaagacgcg cgcggcgatg cccggcttgt cgggaacgcc gaggatcgtg accttcgctt
     1741 cggaggtgtc gtgcgcgatc cccgagatga tcgctcgttc catccgttcg tcctcctctc
     1801 ggacccaggt gccctcgtcg tcggtgaacg aggagcgcac gtgcagcatc acctggtagt
     1861 tccgagcgta ttcgaccgac cgcagcatca gcaccttcgc gccgctggcc gacagctcga
     1921 gcatctcctc gtacgaaacc gcgtgcagct tgcgcgcgga cgggacgagc cgcgggtccg
     1981 cggtgaacac gccggccacg tccgtgtaga tctcgcagta gtccgcgtcg agcgcggccg
     2041 cgagcgccac cgccgtcgtg tcggacccgc cgcggcccag cgtcgtgacg tcgaacgccg
     2101 tcgagacccc ctggaagccc gcgacgatcg cgatgctccc cgcgtcgagg gcctcacgca
     2161 cgcgctgcgc cttgacctcg aggatgcgcg ccttgccgtg cgtcgtgtcc gtcacgatgc
     2221 cggcctgcga gccggtgaac gagatcgcgc cccggccctg ctcgttgatc gccatggaca
     2281 gcagcgccat cgagatgcgc tcgccggccg tcagcagcat gtcgagctcg cgcgggtgcg
     2341 ggaccgtgga gacctcggcg gcgaggtcga ggagctcgtc ggtgctgtgg cccatcgcgc
     2401 tgacgaccac gcacacgcgg tgcccggcct ccgcgcagga gacgacgcgc tcggcgacct
     2461 tctttatgcg atgggcgtcg ccgacggacg tgccgccgta cttctggacg atcgttgcca
     2521 tgcgttcacc ctagacgggg aacgcgtcta gccgcagtgc ttcgagttgc ccttgctgtc
     2581 gtctttcggc acctcgcagt cgcggccgcg gccgggctgc gggtcgccct ggacgtgctc
     2641 gtcgtcctgc gggtccggcg tcggcttcgg cttcgaggtc ggttgcgcgg gccggggcgg
     2701 ctgtcccgtc cgggccggag ggctcgtcgg cggcgtcgcc gtgttcgttc cgctcggcgt
     2761 cgtcttcggg cgtgctccgc gcgtgaggcg cgagccgagg acgaccgtgt cgggacccga
     2821 cttctccttc tgcgcgacgg gcttcggctt cgtcgccggc ttcgagtcgg cgacgtcctc
     2881 caccggctcg aggtcgtcgg tctgcgtcga gaggtcgacg acgtcggact cctgcgggtc
     2941 gtccgcacgc tcgatcgcga tcttcggtcc gtcggccgga cccgcgccgg ggatgaagcc
     3001 gcccatcaac gcggccgcgc ccagcgcgcc gagcgcgggg gtgacgttgc cgacgccgag
     3061 gcgcttcgcc tggacggcgg ccttgcggaa gagccacgag ggggccgcga acagcgccat
     3121 gagccccgcc accgttgcac gggtcggcag cgcggcgacg aacgcctcga cgaccatcgg
     3181 gtcgaactgg ataccggcct cggcccacag cacgtcgacg gccttgtcgc gtccga
//
LOCUS       DASQDO010000187         6706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_386786, whole genome shotgun sequence.
ACCESSION   DASQDO010000187 DASQDO010000000
VERSION     DASQDO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386786"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VEV43_05410"
     CDS             <1..904
                     /locus_tag="VEV43_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HYP22991.1"
                     /translation="LDRFAIDYALCMYCGICVEVCPYDALFWSREFEYAEYDIRQLTH
                     EKERLSDWMATVLPPPPLEQGALLPGMPEPQAAPAQTVAATGARPPTTTSSAVQTRPE
                     QAEDAPKAGTFATEAGRTVDTVPDSKTPEPATGASKSDAAQMESPPEQPAKMSGETVA
                     EGAPASPKAESVPAEEKVDPKTPAPTGASVDLTDLERSRFQAQPGPNVDVPHEEPKPV
                     ALERLEDPEEVYRQVLEEQRAKGSSPQVAEARAKVARVKAERGIRRAGTPEARPTPPV
                     QGGAPKAEEPQPPQGPVAPEDGES"
     gene            904..1413
                     /locus_tag="VEV43_05415"
     CDS             904..1413
                     /locus_tag="VEV43_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYP22992.1"
                     /translation="MEVYEILFWLFAAVMGVSAIRVVTTSNVVHAALYLVATLLGAAI
                     MYVLLFAEFVAWVQVLVYVGAIVVLMLFGLMLTRAPIGKGSFDNDQRPLAAICALAVF
                     VVSSVIMVDAFEGQEIDFTREAGTTSEQVGNAIFSDFVLPFEVVSVLLLAALVGSVVI
                     ARRDEEAAD"
     gene            1414..1719
                     /gene="nuoK"
                     /locus_tag="VEV43_05420"
     CDS             1414..1719
                     /gene="nuoK"
                     /locus_tag="VEV43_05420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYP22993.1"
                     /translation="MPVSYFLVLSALLFSIGIYGVLARQNAVLILLSIELMLNAVNIN
                     LVAFSSMLADAGLDGQVFALFVIAVAAAEVGIGLAIVILMFRNRQTVDVDEMSLMKW"
     gene            1716..3731
                     /gene="nuoL"
                     /locus_tag="VEV43_05425"
     CDS             1716..3731
                     /gene="nuoL"
                     /locus_tag="VEV43_05425"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYP22994.1"
                     /translation="MTDFLLLAAEEAGHEVHKGTFTEYMWIVPLLPFLSAIVIGFFGK
                     KMKNRGHAVGIAAVAIGFALSLVGFAELAAGNAHVEKAWTWFEFPGGFELEFGMNYDF
                     LAGVMFVVVTFISMLVQIYSTGYMHDDERYTWFYAVLSLFTGSMLFLVIANNLTQMLV
                     GWEGVGVCSYFLIGHYWEEKENSSAAMKAFITTRVGDIGFMFGIFALFVLAGTFNVGE
                     INHLAEEGEIGGTALTVAALLLFCGAVGKSAQFPLHVWLPDAMAGPTPVSALIHAATM
                     VVAGVYLVARMFPLFEHAGAALDVVAIIASITMLMAAVLALVQDDIKRVLAYSTISQL
                     AYMMAALGVGAYTAGVFHLFTHAMFKALLFLGSGSLIHAVHSNNMSDMGGLRKYMPHT
                     FRTFIIGSLALAGIFPLAGFFSKDEIIGGALRAASEGHRATAWIVLVSAVVTAFLTAL
                     YMTRACVKTFWGEYRGHGHPHESAGSMVTPLWILAIASVTVGFLGVPVIGPVHDWLHL
                     PGHELHGFDPFYNLILPAGSVLLALSAIFIGWQVFHKGRLKVDVLGGPFAWAYRFVAN
                     KYYLDELYIRGIVRPIQYSWAKVAYWINQKVIDGIVNGAGTATLGGARVAYTIDQDVV
                     DFAVNGAAGITGASGGLLKYIQTGNVQRYAAVLFAAVAVFVAAIALT"
     gene            3750..5273
                     /locus_tag="VEV43_05430"
     CDS             3750..5273
                     /locus_tag="VEV43_05430"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYP22995.1"
                     /translation="MDWFDDWGLSLTTFLPLVGAIVLMFVPQAKEAAVKMVGSIFAGL
                     AHIAGIVVAMRFDYGESGRLQQVVNLEWIPTIDVNYHVGVDGMSLPLVVLSVLVSFLC
                     MIYMHFHIPEPGKSKAFFALVLLLETGMTGTFIALDLILFFIFFEVVLVPMYFMIGIW
                     GGPRREYAAVKFFLFTLFGSIFMLLGFLALYFQTGTFDMVELRELGAAGIGSELFQSL
                     VFLGLFLGFAIKVPMWPFHTWLPDAHTEAPTVGSVLLAAILLKMGTYGFIRISLPILP
                     EAAIDYAPIIGVLAVISIIYGSLCCLAQTDLKRLIAFSSVGHMGFVMLGIATLTDAGI
                     NGAIFGMVAHGLITGMLFFLAGSIHERYHTREIAALGGILKQIPRFGATFSYAAIASL
                     GLPGLAGFWGEFLALAGSYDPAAGLDETLFRVLMVGGGIGTVLTAGYLLWTIQRVNLG
                     RVPENFADGHGIHDVSKLEWIAWAPLLLLIVAAGIYPKMLLGVTNEAVDVLLGAFGA"
     gene            5270..>6706
                     /locus_tag="VEV43_05435"
     CDS             5270..>6706
                     /locus_tag="VEV43_05435"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYP22996.1"
                     /translation="MNVAVDWHALAPETVLTVFACAVLMVDLFLPRDAKWLAMPLSAA
                     GLVATLAAVVSLIGQGDRLTLGGAFEVDTFALAFKGLFCVIGLIVLALSYDYFREGEY
                     AQGEYYFLLLCGLLGTLVMSSSRDLVSIFIAIELISIPGFVMTGLRKRDVKSNEAALK
                     FFLFGVLSSAVMLYGMSLVYGATGGEIQLDAIREALAGRGDDPIVVMSVFFVVVGFAF
                     KISAVPFHFWAPDTYEGAPSPVAAFLSTASKIGGFVGLLVLMFRAFPDVADAWRPAFV
                     ALAVLTMTVGNLIALRQRHIIRLLAYSGIAQSGYVLVAFALITPNGEANQQAFQSAMF
                     YLAIYGIMDAGAFAAAISFARRGGSYFIDDYAGLWQRSPALALLLAGFLVSLAGTPPM
                     AGAWAKLFVFLAAIDADAVWLAVVMGLNAVIAAWYYLAVVKRMFFESPAPEETREIEV
                     PYLLKAAMGVAALLLLVAFVIPRPIADMA"
BASE COUNT          935 a         2264 c         2250 g         1257 t
ORIGIN      
        1 cctcgaccgg ttcgcgatcg actacgcgct ctgcatgtac tgcggaatct gcgtcgaggt
       61 gtgcccctac gacgcgctgt tctggagccg cgagttcgag tacgccgagt acgacatccg
      121 ccagctcacg cacgagaagg agcggctgtc ggactggatg gcgacggtgc tgccgccgcc
      181 cccgctcgag cagggcgcgc ttctgccggg catgccggag ccgcaggccg cgcccgcgca
      241 gaccgtcgcc gccaccggcg cgcggccccc tacgacgacg tcttctgcgg tgcagacccg
      301 tccggagcag gcggaggacg cgccgaaggc cggcacgttc gccaccgagg ccggccgcac
      361 cgtcgacacg gttcccgaca gcaagacgcc ggagcccgcg accggcgcgt cgaagtccga
      421 cgcggcgcag atggagagcc cgcccgagca gcccgccaag atgagcggcg agaccgtcgc
      481 cgagggcgcg ccggcctcac cgaaggccga gagcgtcccg gccgaggaga aggtcgaccc
      541 caagacgccc gcgcccacgg gggcctcggt cgacctcacc gacctcgaga ggtcacgctt
      601 ccaggcccag ccgggaccga acgtcgacgt cccgcacgag gagccgaagc ccgtcgccct
      661 cgagcgcctc gaggaccccg aggaggtcta ccgccaggtg ctcgaggagc agcgtgcgaa
      721 ggggtcgtcg ccgcaggtcg ccgaggcccg ggcgaaggtc gcgcgcgtga aggccgagcg
      781 cgggatccgc agggccggga cgccggaggc acgcccgacg ccccccgtcc agggcggcgc
      841 cccgaaggcc gaggagcccc agcccccgca ggggccggta gcgcctgaag acggggagag
      901 ctgatggagg tctacgagat cctcttctgg ctcttcgccg ccgtcatggg cgtctctgcg
      961 atccgcgtcg tgacgacgag caacgtcgtc cacgcggccc tctacctcgt cgccaccctg
     1021 ctcggcgccg cgatcatgta cgtgctgctg ttcgccgagt tcgtcgcctg ggtccaggtc
     1081 ctcgtctacg tcggcgcgat cgtcgtcctg atgctgttcg ggctcatgct cacgcgggcg
     1141 ccgatcggca agggcagctt cgacaacgac cagcggccgc tcgcggcgat ctgcgcgctc
     1201 gcggtcttcg tggtgtcgag cgtgatcatg gtcgacgcgt tcgagggcca ggagatcgac
     1261 ttcacgcgcg aggccggcac gacctcggag caggtcggca acgccatatt ctccgacttc
     1321 gtcctgccgt tcgaggtggt ctcggtgctg ctgctggccg ccctcgtggg ctcggtcgtg
     1381 atcgctcgac gtgacgagga ggctgcggac tagatgccgg tctcgtactt cctggtgctc
     1441 tcggcactgc tgttctcgat agggatctac ggggtgctcg cgcgccagaa cgcggtgctg
     1501 atcctgctct cgatcgagct gatgctgaac gccgtgaaca tcaacctcgt cgcgttctcg
     1561 tcgatgctgg ccgacgccgg cctcgacggt caggtgttcg cgctgttcgt catcgcagtg
     1621 gccgcggcgg aggtcggcat cggcctcgcg atcgtcatcc tcatgttccg taaccgccag
     1681 accgtggacg tggacgagat gtccctgatg aagtggtgac cgacttcctg ctcctggccg
     1741 ccgaggaggc cggccacgag gtccacaagg ggacgttcac ggagtacatg tggatcgtcc
     1801 ccctgctgcc gttcctctcg gcgatcgtca tcgggttctt cggcaagaag atgaagaacc
     1861 ggggccacgc cgtcgggatc gctgccgttg ccatcggctt cgccctctcg ctggtcggct
     1921 tcgccgagct cgccgccggc aacgcacacg tcgagaaggc gtggacgtgg ttcgagttcc
     1981 cgggcggctt cgagctcgag ttcgggatga actacgactt cctcgccggc gtgatgttcg
     2041 tcgtcgtgac gttcatctcg atgctcgtgc agatctactc gacgggctac atgcacgacg
     2101 acgagcgcta cacgtggttc tacgcagtcc tgtcgctgtt taccggctcg atgctgttcc
     2161 tcgtgatcgc gaacaacctc acccagatgc tcgtcgggtg ggagggcgtc ggcgtgtgct
     2221 cctacttcct catcggccac tactgggagg agaaggagaa ctcgtcggcc gcgatgaagg
     2281 cgttcatcac cacccgcgtc ggcgacatcg ggttcatgtt cgggatcttc gccctgttcg
     2341 tgctcgcggg gacgttcaac gtcggcgaga tcaaccacct cgccgaggag ggcgagatcg
     2401 gcggcaccgc gctgacggtc gcggccctcc tgctgttctg cggggcggtc ggcaagtcgg
     2461 cccagttccc gctgcacgtc tggctgcccg acgccatggc gggccccacc ccggtctccg
     2521 ctctgatcca cgcggcgaca atggtcgtcg ccggcgtcta cctcgtcgcg cgcatgttcc
     2581 cgctgttcga gcacgcgggg gccgctctcg acgtcgtcgc gatcatcgcc tcgatcacga
     2641 tgctgatggc ggccgtgctc gcgctcgtgc aagacgacat caagcgcgtg ctcgcgtact
     2701 cgacgatcag ccagctcgcc tacatgatgg cggcgctcgg cgtcggcgcc tacaccgccg
     2761 gcgtcttcca cctgttcacg cacgcgatgt tcaaggcgct gctcttcctt ggctccggct
     2821 ccctgatcca cgcggtgcac tcgaacaaca tgagcgacat gggcggcttg cgtaagtaca
     2881 tgccgcacac gttcaggacg ttcatcatcg ggtcgctcgc tctcgccggg atcttcccgc
     2941 tggccgggtt cttctcgaag gacgagatca tcggcggcgc gctgagggcc gccagcgaag
     3001 gacatcgcgc cacggcatgg atcgtgctgg tctccgccgt cgtcaccgcg ttcctgacgg
     3061 cgctgtacat gacgcgagcg tgcgtgaaga cgttctgggg cgagtaccgg gggcacggcc
     3121 atccgcacga gagcgcgggt tcgatggtca cgccgctgtg gatcctcgcg atcgcgtcgg
     3181 taacggtcgg tttcctaggg gtccccgtga tcggtccggt ccacgactgg ctccacctcc
     3241 ccggccacga gctccacggg ttcgatccct tctacaacct catcctgccg gcgggctcgg
     3301 tcctgctcgc gctgagcgcg atcttcatcg gctggcaggt cttccacaag ggccgcttga
     3361 aggtcgacgt cctcggcgga ccgttcgcgt gggcgtaccg cttcgtggcg aacaagtact
     3421 acctcgacga gctctacatc agggggatcg tccggcccat ccagtactcg tgggccaagg
     3481 tcgcctactg gatcaaccag aaggtcatcg acgggatcgt caacggagcg ggtaccgcga
     3541 ccctcggcgg cgccagggtc gcctacacga tcgaccagga cgtcgtcgac ttcgccgtca
     3601 acggcgcggc tggcatcacc ggcgcgtccg gcggcttgtt gaagtacata cagaccggga
     3661 acgtccagcg atacgcggcg gtgctgttcg cggcggtcgc ggtgttcgta gccgcgatag
     3721 cgcttaccta gggagggttc gagagggcta tggactggtt cgacgactgg gggctgagcc
     3781 tcacgacgtt cctgccgctc gtcggcgcga tcgtgctcat gttcgtgccg caggcgaagg
     3841 aagcggcggt caagatggtc ggctcgatct tcgccggcct cgcccacatc gcgggaatcg
     3901 tcgtcgcgat gagattcgac tacggcgagt ccggcaggct gcagcaggtc gtcaacctcg
     3961 agtggatccc gacgatcgac gtcaactacc acgtaggcgt cgacgggatg tcgctgccgc
     4021 tcgtcgtgct gtcggtcctc gtctcgttcc tctgcatgat ctacatgcac ttccacatcc
     4081 ccgagccggg gaagtcgaag gcgttcttcg cgttggtgct gctgctcgag accgggatga
     4141 cggggacgtt catcgccctc gacctcatcc tgttcttcat cttcttcgag gtcgtgctcg
     4201 tgccgatgta cttcatgatc ggcatctggg gcgggccgcg gcgtgagtac gccgccgtca
     4261 agttcttcct cttcacgctg ttcggctcga tcttcatgct gctgggcttc ctggcgctgt
     4321 acttccagac cggcacgttc gacatggtcg agctgcgaga gctgggggcc gcaggcatcg
     4381 ggagcgagct gttccagtcg ctcgtgttcc tcgggttgtt cctcgggttc gcgatcaagg
     4441 tccccatgtg gccgttccac acgtggctcc ccgacgcaca taccgaggct ccgacggtcg
     4501 gctcggtcct gctggccgcg atcctgctga agatggggac ctacggcttc atccggatct
     4561 cgctgccgat cctgcccgag gccgcgatcg actacgcgcc gatcatcggg gtgctggcgg
     4621 tgatctcgat catctacggg tcgctgtgct gtctcgccca gaccgacctc aagcggctga
     4681 tcgcgttctc gtccgtcgga cacatgggct tcgtgatgct cggcatcgcg acgctcaccg
     4741 acgcgggcat caacggcgcg atcttcggga tggtcgccca cggcctcatc accggcatgc
     4801 tgttcttcct cgccggctcg atccacgagc gctaccacac gagggagatc gcggccctcg
     4861 gcgggatcct caagcagata ccgcgcttcg gcgcgacgtt cagctacgcg gcgatcgcgt
     4921 cgctcggcct tccggggctc gccggcttct ggggtgagtt cctcgcgctc gccggctcgt
     4981 acgacccggc cgccgggctc gacgagacgc tgttccgcgt cctgatggtc ggcggcggca
     5041 tcggcaccgt gctgaccgcc ggatacctcc tgtggacgat ccagcgcgtg aacctcggcc
     5101 gggtgccgga gaacttcgcc gacgggcacg gcatccacga cgtgtcgaag ctcgagtgga
     5161 tcgcatgggc accgctgctc ctcctcatcg tggcggcagg catctatccg aagatgctcc
     5221 tcggcgtcac caacgaggcc gtggacgtgc tcctgggggc cttcggcgcg tgaacgtagc
     5281 cgtcgactgg cacgcgctcg cgccggagac ggtgctcacc gtcttcgcgt gcgccgtcct
     5341 gatggtcgac ctcttcctgc cgcgcgacgc gaagtggctc gcgatgccgc tgtcggcggc
     5401 gggcctggtg gcgacgctcg ctgcggtcgt gtcgctgatc gggcagggcg accgcctcac
     5461 gctcggcggc gcgttcgagg tcgacacgtt cgcgctcgcg ttcaagggcc tgttctgcgt
     5521 catcggcttg atcgtcctcg cgctgtcgta cgactacttc cgcgagggtg agtacgcgca
     5581 gggcgagtac tacttcctgc tgctgtgcgg gctgctcggg acgctcgtca tgtcgagctc
     5641 ccgtgacctc gtgtccatct tcatcgcgat cgagctgatc agcatccccg gcttcgtgat
     5701 gacggggctg cggaagcggg acgtgaagtc gaacgaggcc gcgctgaagt tcttcttgtt
     5761 cggcgtcctg tcctcggccg tgatgctcta cgggatgtcg ctcgtgtacg gcgcgacggg
     5821 cggcgagatc cagctcgacg ccatccggga ggcactcgcc gggcgtggcg acgaccccat
     5881 cgtggtcatg agcgtgttct tcgtcgtcgt ggggttcgct ttcaagatct cggcggtgcc
     5941 gtttcacttc tgggcccccg acacctacga gggggcaccg tcgcccgtcg ccgcgttcct
     6001 ctcgaccgcg tccaagatcg gcggcttcgt cgggctgctc gtgctgatgt tccgggcctt
     6061 ccccgacgtc gccgacgcgt ggcggccggc cttcgtcgcg ctcgccgtgc tgacgatgac
     6121 ggtcggcaac ctcatcgcgc tgcggcagcg gcacatcatc cggctgctcg cgtactccgg
     6181 gatcgcgcag tccggctacg tgctcgtcgc gttcgcgctg atcacgccga acggcgaagc
     6241 gaaccagcag gcgttccagt cggcgatgtt ctacctggcg atctacggga tcatggacgc
     6301 aggtgcgttc gcggcggcga tctcgttcgc gcggcgcggc ggttcgtact tcatcgacga
     6361 ctacgcgggg ctgtggcagc gctcgcccgc gctcgcgctc ttgctcgcgg gcttcctggt
     6421 ctcgcttgcc gggacgccgc cgatggcggg ggcctgggcg aagctgttcg tcttcctcgc
     6481 cgccatcgac gccgacgcgg tctggctggc ggtggtcatg ggcctgaacg cggtgatagc
     6541 ggcctggtac tacctggcgg tcgtgaagag gatgttcttc gagtccccgg cgccggaaga
     6601 gacgcgcgag atcgaggtcc cgtacctgct gaaggccgcg atgggcgtag ccgcgctatt
     6661 gctgctggtg gccttcgtga taccgcgccc gatagccgac atggcg
//
LOCUS       DASQDO010000188         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_203327, whole genome shotgun sequence.
ACCESSION   DASQDO010000188 DASQDO010000000
VERSION     DASQDO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203327"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VEV43_05440"
     CDS             <1..290
                     /locus_tag="VEV43_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009113530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HYP22997.1"
                     /translation="ALPAWDDALPFLVSHLPEAATWSATGIGRAHLPVAEQTIALGGH
                     VRTGFEDVVYYAKGVKARSNAQLVERVAEMARSAGREVATPAIAREMLGLR"
     gene            303..848
                     /locus_tag="VEV43_05445"
     CDS             303..848
                     /locus_tag="VEV43_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HYP22998.1"
                     /translation="MTDQKRRLVERILDTAYVEGLGDRPLEELRSMRDECKEAETEIS
                     FERRLAQARIDILSAELDRRSGTGDNSDLMSRLPQILATEGRSDTGLPLPSRAPDFSI
                     PRNADIPRRRIEEIVGEQTLSRLPSLPTEELRGILGSLGDHEKTLSTRRKVVQEVMDT
                     VQAEIVRRYVSGEADPTAALG"
     gene            complement(858..1634)
                     /locus_tag="VEV43_05450"
     CDS             complement(858..1634)
                     /locus_tag="VEV43_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP22999.1"
                     /translation="MSAERTPRRANLATMVGSSLAVVAAGSLLAFSTLAEQAGLEGLA
                     TRGIEPAQPGRVRSGEHAITIPAPATAPPTDLRVEVSDFGREGGRARVRTVAPVEPPA
                     PAPLPPREPDEPEVRRIDHRARVAVDGPDVDDKVEKRDGPPYGHAYGHRKNKHGKSPK
                     HERKNESPEAPVYARTANDEAPPKKSKAPKLQKVKKVKKAKGAAHPGNGKAKGHSKHA
                     AGPVAAHSNGKAKGHSNGKAKGHAKHAHKGKGKGNSKHGG"
     gene            1837..2202
                     /locus_tag="VEV43_05455"
     CDS             1837..2202
                     /locus_tag="VEV43_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP23000.1"
                     /translation="MKLGELGAFIREQRRASRLSLRKLSDLAGISNPYLSQIERGLRK
                     PSAEILQAIAKALRISAETLYVQAGILDDEREHDLVTEILKDTSINERQKQVLIDIYR
                     SFLEQGSGGETDPEEVTSR"
     gene            2199..2492
                     /locus_tag="VEV43_05460"
     CDS             2199..2492
                     /locus_tag="VEV43_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23001.1"
                     /translation="MTRTKEMLYAAIGAGDLAVEKVKDVRRIVDPSVYGDLVGRGRDL
                     TGRIAKAAPTKRAVDRTKTARSQAKAAATSVRKAVRANATAARSAAEKVAKAS"
     gene            2602..3639
                     /locus_tag="VEV43_05465"
     CDS             2602..3639
                     /locus_tag="VEV43_05465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP23002.1"
                     /translation="MSQTIAPEETAIDVRVHTTPAAFDLAAWPGLLKRDPDRHVFATP
                     EWSRVWWEEFAGDKELLVLEMTRGDETVAIVPLYRKAGAGRRILQFVGGIDLTDYLGP
                     ICAAEDRRAVAGALVAWLRSEEAGWDELDAHNMPVPLGFAEYLVERADTCDFRFALDQ
                     EETAAVLPLPGDWETYLMRLDSKERHELKRKRRRLTRDHPDAVFRTATPETLAADMKT
                     FVDMHRGAEGHKGHFMRPEIATFFERMAGAFMPLGWLRLDFLEVGGRAVAATFAFELD
                     GVFYLYNSAYEPDAARVSPGLMLVSWLVEGCIERGFEKFDFLRGPERYKYQLGSQAVP
                     LNNVRVIRSDG"
BASE COUNT          539 a         1348 c         1304 g          594 t
ORIGIN      
        1 gcgcgctccc ggcgtgggac gacgccctcc cgttcctcgt ctcccacctg cccgaggccg
       61 ccacgtggag cgcgaccggc atcggccgcg cccacctccc ggtcgcggag cagacgatcg
      121 cgctcggcgg ccacgtccgg acgggcttcg aggacgtcgt ctactacgcg aagggcgtca
      181 aggcgcggtc gaacgcgcag ctcgtcgaac gggtcgcgga gatggcgcgg agcgcagggc
      241 gcgaggtcgc gacgcctgcg atcgcccgcg agatgctggg actccgctga taccctgacc
      301 tcatgaccga tcagaagcgc cgcctcgtcg agcgcatcct cgacaccgcc tacgtcgagg
      361 gcctgggcga caggccgctc gaggagctgc ggtcgatgcg cgacgagtgc aaggaggccg
      421 agaccgagat ctcgttcgag cggcgcctcg ctcaggctcg catcgacatc ctctcggccg
      481 agctcgaccg caggtccggt accggggaca actcggacct gatgtcgcgg ctgccgcaga
      541 tcctcgcgac cgaggggcgc agcgacacgg ggctcccgtt gccgtcgagg gccccggact
      601 tctcgatccc gcgcaacgcc gacatcccgc ggcgccgcat cgaggagatc gtcggcgagc
      661 agacgctgtc ccggctcccg tcgctgccga cggaggagct gcgggggatc ctgggctcgc
      721 tgggcgacca cgagaagacg ctgtctacgc ggcgaaaggt ggtccaggag gtaatggaca
      781 ccgtgcaggc cgagatagtg cgccgctacg tgagcggcga agcggacccg accgcggccc
      841 tgggctaacc cgcggcgcta gccgccgtgc ttcgagttgc ccttgccctt gcccttgtgg
      901 gcgtgcttgg cgtggccctt ggccttgccg ttcgagtggc ccttggcctt gccgttcgag
      961 tgcgcggcga cggggccggc ggcgtgcttc gagtggccct tcgccttccc gttgcccggg
     1021 tgcgccgcgc ccttggcctt cttgaccttc ttcaccttct gcagcttcgg cgccttcgac
     1081 ttcttcggcg gagcctcgtc gttcgccgtg cgcgcgtaga ccggcgcttc gggtgactcg
     1141 ttcttccgct cgtgcttcgg cgatttgccg tgcttgttct tgcggtgacc gtaggcgtgg
     1201 ccgtagggcg ggccgtcccg cttctcgacc ttgtcgtcga cgtcgggccc gtcgaccgct
     1261 acgcgtgcgc ggtggtcgat gcgccgtacc tcgggctcgt ccggctcgcg cggcggcagc
     1321 ggcgcggggg ccgggggctc gaccggcgca accgttcgca ctcgtgcgcg acccccttcc
     1381 cgtccgaagt ccgacacctc gacgcgcagg tccgtcgggg gcgcggtcgc gggggccggg
     1441 atcgtgatcg cgtgctcgcc ggatcggacc cggcccggct gcgcgggctc gatgcctcgc
     1501 gtcgcgagtc cctccaagcc ggcctgctcc gcgagcgtcg agaacgcgag caggctcccc
     1561 gcggcaacta ccgcgaggct gctacccacc atcgtcgcca ggttcgcccg gcgaggcgtt
     1621 ctctctgcac tcatctgagt tgggttctcc actcgggacc gcatgcctcc cgatgcgtcg
     1681 tcgaacggtc actcaccgtg attggacgca cggtacagcg actacgttcc gatagaagac
     1741 atatcggaca gttagggatt cttcttaaca cggggatcaa gggcgtgctt gcaactgtta
     1801 tgcacgcgtg ctatagctgt tagcgtgtct tcgaggatga agctgggcga gctgggcgcc
     1861 ttcatccgcg agcagcggcg ggcgagccgc ctgtcgctcc gcaagctctc cgacctcgca
     1921 ggcatctcga acccgtacct gtcccagatc gagcgggggc tgcggaagcc ctcggccgag
     1981 atcctccagg cgatcgccaa ggcgttgcgg atctcggcgg agacgctgta cgtgcaggcc
     2041 gggatcctcg acgacgagag ggagcacgac ctcgtcaccg agatcctcaa ggacacctcg
     2101 atcaacgagc gccagaagca ggtgctcatc gacatctacc gctcgttcct cgagcagggg
     2161 agcggcggcg agaccgaccc cgaggaggtg acaagcagat gaccaggacc aaggagatgc
     2221 tctacgccgc gatcggcgcc ggcgacctcg ccgtcgagaa ggtcaaggac gtgcggcgga
     2281 tcgtcgaccc gagcgtgtac ggcgacctcg tcggccgcgg ccgtgacctc accgggcgga
     2341 tcgcgaaggc cgccccgacc aagagggcgg tcgaccggac gaagacggct cgctctcagg
     2401 ccaaggcggc cgctacgagc gtccgcaagg ccgtccgggc caacgccacg gcggcccgct
     2461 cggctgccga gaaggtggcc aaggcctcct gacccgatct cggacgaggg gcggctccgg
     2521 ccgccccttt tcgcgctctc ttgcgcccgc atgggtatag gggacggaac aacgccgaga
     2581 cctgtggggt tgacgcacga catgagccag acgatcgctc ccgaggagac ggctatcgac
     2641 gtccgcgtgc acacgacgcc ggccgcgttc gaccttgcgg cctggcccgg cctcctgaag
     2701 cgcgaccccg accggcacgt cttcgccacg ccggagtgga gccgcgtctg gtgggaggag
     2761 ttcgccggcg acaaggagct actcgtcctc gagatgaccc gcggcgacga gacggtcgcg
     2821 atcgtccccc tgtaccggaa ggctggggcg ggccgccgga tcctccagtt cgtcggcggg
     2881 atcgacctga ccgactacct cggcccgatc tgcgccgccg aggaccgccg cgcggtcgcc
     2941 ggcgccctcg tcgcctggct gcgctcggag gaggcgggct gggacgagct cgacgcccac
     3001 aacatgccgg tcccgctcgg gttcgccgag tacctcgtcg agcgtgccga cacctgcgac
     3061 ttccgcttcg ctctcgacca ggaggagacc gccgcggttc tgccgctccc cggcgactgg
     3121 gagacgtacc tcatgcgcct cgactcgaag gagcgccacg agctcaagcg caagcggcgc
     3181 cgcctgaccc gcgaccatcc cgacgcggtc ttccgcacgg cgacgcccga gaccctcgcc
     3241 gccgacatga agacgttcgt cgacatgcac cggggggccg agggccacaa gggccatttc
     3301 atgcgccccg agatcgcgac gttcttcgag cggatggccg gcgcgttcat gccgctgggc
     3361 tggctgcggc tggacttcct cgaggtcggg ggccgggcag tcgccgccac cttcgccttc
     3421 gagctcgacg gcgtcttcta cctgtacaac tcggcctacg agccggatgc ggcccgcgtg
     3481 tcgcccggct tgatgctcgt gtcctggctc gtcgagggct gcatcgagcg tggcttcgag
     3541 aagttcgact tcctgagggg gccggagcgc tacaagtacc agctcggctc ccaggccgtg
     3601 ccgctcaaca acgtccgcgt gatccgttcg gacgggtgaa gcggctagcg gttctcgcgt
     3661 accactcctc gccgctcggc ccccccggcg ccggcgactc cggcggcatg accgtctacg
     3721 tccgcgacct ggcgtcggcg ttcgcacgcc gcggcatccg gaccgacgtc tacacgcgcg
     3781 ccgac
//
LOCUS       DASQDO010000189         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_937361, whole genome shotgun sequence.
ACCESSION   DASQDO010000189 DASQDO010000000
VERSION     DASQDO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937361"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1235
                     /locus_tag="VEV43_05470"
     CDS             111..1235
                     /locus_tag="VEV43_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HYP23003.1"
                     /translation="MAMPTVEQISAVLGEIQDPELHRGLNELNMVRDVTVSGTEVRTL
                     IALTIPGCPLKDYFYTVIPARLKEEFPEISHVQVDLTSMTEEERAALVGGLRKETPAP
                     FARSDSSTQVIAIGSGKGGVGKSTTTVNIAASLAKLGHSVGLLDADVWGFSIPRMLGI
                     HERPTVVDDMIMPPTMFGVKVVSIGLFTTEDNPVVWRGPMLHKALQQFLSDVHWDEPD
                     YLLVDLPPGTGDVSISIAQFLPGASMLVVTTPQAVAEKVAQRAGFMAEKTGLDVVGVV
                     ENMSYFRGDDGKVYEIFGSGGGATLAQTLEVPLLGQIPIDPALREYADDGAPIVLQAP
                     DSEVAQALEQTAKELVNLLPPKPKPAKRINLPLMAGPHRH"
     gene            complement(1232..1987)
                     /locus_tag="VEV43_05475"
     CDS             complement(1232..1987)
                     /locus_tag="VEV43_05475"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HYP23004.1"
                     /translation="MSFLDAVGRRDYRVAIVLGSGLAPVAGALTGAEPVPYSSFDGWP
                     RTRVPGHEGALYAGEIEGIPALVLAGRVHLYEGHPSSAVTHAVDEVVAAGCRTVVLTN
                     AAGGIAESFGVGEPVLISDHLNLTGSSPLIGAPTFVDLSAVYDPELRTLARTVDPSLQ
                     EGVYAGVVGPAYETPAEIRMLRMLGADLVGMSTVHEAIAAHALGARVLGISVVTNRAA
                     GLGTRLDHAEVQRAGAEAAGRMESLLRGVVAQL"
     gene            complement(1984..2391)
                     /locus_tag="VEV43_05480"
     CDS             complement(1984..2391)
                     /locus_tag="VEV43_05480"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HYP23005.1"
                     /translation="MTERVDWDELRAEASEAAGRAYAPYSHTPMGAAALLDDGSILTG
                     CSVENASFGLSLCAECGLVSALYDSGGGRFVGITLVDLDGNLLTPCGRCRQLLFEAGG
                     PELLVDSADGPRRLDQLLPDGFSADQLAETTGI"
BASE COUNT          373 a          878 c          810 g          355 t
ORIGIN      
        1 gacgtggcct cgatccgttt gatcccacag gggctcgacg tcctggtgta gttgcgcggc
       61 ccccttcgct tagggttttc ggcagtcgaa tcggacagga gagccttcac atggcaatgc
      121 ccaccgtcga acagatcagc gccgtcctcg gcgagatcca ggaccccgag ctccaccggg
      181 gcctcaacga gctgaacatg gtgcgggacg tcactgtctc cggcaccgag gtccggacgc
      241 tgatcgcgct gacgatcccc ggctgtcccc tcaaggacta cttctacacg gtgatcccgg
      301 cgcgcctgaa agaggagttc cccgagatct cgcacgtcca ggtcgacctc acgtcgatga
      361 ccgaggagga gcgtgcggcc ctcgtcggcg ggctccgcaa ggagaccccg gcccccttcg
      421 cgcgcagcga ctcgtccacg caggtgatcg cgatcggctc gggcaagggc ggcgtcggca
      481 agtcgacgac gacggtgaac atcgcggcgt cgctcgcgaa gctcgggcac tccgtcgggc
      541 tcctcgacgc cgacgtgtgg ggcttctcga tcccgcgcat gctcggcatc cacgagcgtc
      601 cgacggtcgt cgacgacatg atcatgccgc cgacgatgtt cggcgtgaag gtcgtgtcga
      661 tcgggctgtt cacgaccgag gacaacccgg tcgtgtggcg gggaccgatg ctgcacaagg
      721 cactccagca gttcctctcc gacgtccact gggacgagcc cgactacctg ctcgtcgacc
      781 tccccccggg gaccggtgac gtgtcgatct cgatcgcgca gttcctgccg ggggcgagca
      841 tgctcgtcgt cacgacgccg caggccgtcg ccgagaaggt cgcgcagcgc gccggcttca
      901 tggccgagaa gaccgggctc gacgtcgtcg gcgtcgtcga gaacatgtcg tacttccgcg
      961 gcgacgacgg gaaggtctac gagatcttcg gctcgggcgg gggcgcgacg ctcgcgcaga
     1021 cgctcgaggt gccgttgctc ggccagatcc ccatcgaccc ggccctgcgc gagtacgcag
     1081 acgacggcgc gccgatcgtg ttgcaggcac ctgacagcga ggtggcccag gctctcgagc
     1141 agacggcgaa ggagttggtg aacctgctcc cgccgaagcc caagccggcg aagaggatca
     1201 acctgccgct gatggcgggc ccgcaccgcc actagagctg cgccaccact cctctcagca
     1261 gggactccat ccggcccgct gcttcggccc ccgctcgttg cacttccgcg tggtccaggc
     1321 gcgtccccag gccggccgct cggttcgtca ccaccgagat ccccaggacc cgggccccca
     1381 gggcgtgggc tgctatcgcc tcgtgcaccg tcgacatccc gaccaggtcc gcacctagca
     1441 tcctcagcat gcggatctct gccggcgtct cgtaggccgg gcccaccacg cccgcgtaga
     1501 cgccttcttg caggctcggg tccacggtcc tcgccagcgt gcgcagctcg gggtcgtaga
     1561 cggcgctcag gtccacgaac gtcggcgccc cgatcaacgg gctcgatccc gtcaggttga
     1621 ggtggtccga gatcagcacg ggctcaccga cgccgaacga ctcggcgatc ccaccggcgg
     1681 cattcgtcag cacgaccgtc cggcaccccg ctgcgacgac ctcgtcgacc gcatgcgtga
     1741 ccgcgctgga cggatgcccc tcgtagaggt ggacccggcc cgcgagcacc agcgcgggga
     1801 tgccttcgat ctcgccggca tagagcgcgc cctcgtgccc cggcacccgc gtgcgcggcc
     1861 aaccgtcgaa gctcgagtag gggaccggct cggctcccgt caacgcgccc gcgacgggcg
     1921 cgagcccgga gcccagcacg atcgcaacgc ggtagtcgcg gcgaccgacg gcgtcgagga
     1981 agctcagatg cccgtcgtct ccgcgagctg gtcggcggag aacccgtcgg gaaggagctg
     2041 gtcgagccgg cgcgggccgt cggccgaatc gacgagcagc tccggccccc ccgcctcgaa
     2101 cagcagctgc cggcaacgcc cgcacggcgt cagcaggttc ccgtcgaggt cgaccagcgt
     2161 gatgccgacg aaccgcccgc cgccggagtc gtagagggcc gagaccagcc cgcactcggc
     2221 gcagaggctc agcccgaacg acgcgttctc gacgctgcag ccggtgagga tgctgccgtc
     2281 gtcgaggagc gcggccgctc ccatcggcgt gtgcgaatag ggggcatagg cgcggccggc
     2341 ggcttcgctc gcctcggccc ggagctcgtc ccagtccacg cgctcggtca tcgggccatt
     2401 gtgttcgctc ggcttc
//
LOCUS       DASQDO010000190         9176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_754668, whole genome shotgun sequence.
ACCESSION   DASQDO010000190 DASQDO010000000
VERSION     DASQDO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754668"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..447
                     /locus_tag="VEV43_05485"
     CDS             217..447
                     /locus_tag="VEV43_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYP23006.1"
                     /translation="MEIFPGITVDPDVRFGKPCVTGTRVDVATIVGAIGAGEPTGAVA
                     EEFGLTLEQVQAALLYAAHVADHAPPAVREVS"
     gene            complement(713..1411)
                     /locus_tag="VEV43_05490"
     CDS             complement(713..1411)
                     /locus_tag="VEV43_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23007.1"
                     /translation="MKKLLTVMVGACLAGSIVGTASAGRPTTVFEDPAGDAGIEGGTA
                     VGENSIPGFDQAGFDLVSGRIARAGRNLEFTVTSAAMPATGSLPEGATFFWNFMSNGT
                     RYRFTIKSQDVGKPDALTQEGTDRVGRVDLEGHFRLETCEEGSEIAGFGWSHCKLVAF
                     EDGSFDPASKSFSVTIPLGDIGASTGSLISPSASGSTRCQPCWIMHTAERTSPPGFIL
                     DGTDWPISYKVPAK"
     gene            complement(1575..1841)
                     /locus_tag="VEV43_05495"
     CDS             complement(1575..1841)
                     /locus_tag="VEV43_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23008.1"
                     /translation="MGDTNTRVVVDEILTTIGSFGGGDSDGGGFDLRPSGRVIKIPPR
                     QDLQVIPAERRDELIVHAIQVMAQDISDEQLREQMTRAAEAALQ"
     gene            1955..3022
                     /locus_tag="VEV43_05500"
     CDS             1955..3022
                     /locus_tag="VEV43_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HYP23009.1"
                     /translation="MSKFVPGVELSRALYVEHVAGLLGGVSHAAALLGEGSEVLGFDT
                     ERSTDHAWGPRLHVFVAAEDVDETRSRIDGGLPDDVLGWPTRVYRWQTDEVAHHVEVE
                     PLGPWLERQLGFDPRNGVTTAQWLATPQQSLLHVTAGPVFADADGELSAVRERLSWYP
                     KDVWLWAMAAAWQRVQDNEHVVGRTAETGDDLGNRLVVAKLVGHLTSLCFLQEKRYAP
                     YVKWVGAAFARLDAAAEIGPSFDRALSSPDGVEREDAVASALEAVARRHNDLGLTEHL
                     DPATGPFNVKINDAVRPFRVLNANRFVNACSAAIEDDQVRDVAPVGAIDQLLDPNDLI
                     TNFTDWPRELEAVYARKLGES"
     gene            3025..3738
                     /locus_tag="VEV43_05505"
     CDS             3025..3738
                     /locus_tag="VEV43_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23010.1"
                     /translation="MRLRIAGIVLSVLLGGAAVAVIVLDGEEEPFGRGDSLLPSFSAG
                     YQCTDPRDELAAEELSYVLGYSREQWSARVALSVAGGETRGPCSVQLGVPTGSEDLES
                     EDANVLLERHGSPSSGIASVPLRDRARDYVMNVTLPRDFELFHALGFGKYVFELDFFA
                     PGEGESFKQATVDLRLPEGYSIDEALPPGDNPGSERSRKWTFKADRDQQAVVTFEHDR
                     LRTAVDLAPEVAFGALLLV"
     gene            3745..4215
                     /locus_tag="VEV43_05510"
     CDS             3745..4215
                     /locus_tag="VEV43_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23011.1"
                     /translation="MTLPRRSDERAEPEPAVASQPMPIEATQPEPEPELVAEPEPTPE
                     PEPEPLPEPEPTPEPEPEPLPEPEPLPEPEPQPVAAAIPAPEPQPALPAPVPPAPPAL
                     APPAPAPRPLSPAPRPPAPLPERVPSRTARAAGAGAGLLAAALFFRLVRRLRNR"
     gene            4388..7819
                     /locus_tag="VEV43_05515"
     CDS             4388..7819
                     /locus_tag="VEV43_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HYP23012.1"
                     /translation="MSARARLSAAVLVLSLVATLVAAIVPREAMAVPNPVATDEPTYQ
                     LFGRVFPDPQGCLAHDVPDEDVNGIKDTPKGVSPWAKGRVCAGQFLSYEEVIEGAKFM
                     SRRFPDFVEVVRLDQAFDNPNYRSAGIPRSISLENGTAKVMGRDRRPLYMFRVTDRTS
                     DIPPRKREHFAFSLSIHGPERAGLEGGIRAMEDLVTWAACERSDYAATTPACEAEGAF
                     PKKIVESATDRPVPTAGEALKRSVLYFTLPNPDGWGRGQTAPVEIEDGGPNVSYLPGM
                     AFQRFNGNGVDLNRDWPTIGYTLKSYSPGSEPETKAFADALSRVKARTEAGRFAGGLD
                     LHGMLTAKAFSYTLIGAGQRDYRKNAVTVETSLRTWEDQTNRLSWSPYVADHNANGEK
                     EPEASPPEICRDTNGTDATPCVADQWGTVIDTIGYQVTGSFGDWFDSPLGLDAVGVNN
                     EMFVSHVAPNSVFEPALEQTHIDGNKGLIFSQIAALLRTSETTFHPGGTTGYVFDPNR
                     LQVPAQPRVPNPGLPAQNDIEVVLPCQDQVAQNADGFCGDGEFVVSGTQTSYEFDVEG
                     PRHEIWNGGITATTTHANANGVSPGALPVIHLDYFDEGQWQPVARSVVGEGHFYHQAG
                     RIVTANDPEPGRWRVRFGTPLDSVTRLQVDFNPYTAEASPGQAAIDASSMDFFSELNE
                     FVPEGEKVAPVDPKVVYRSVLALRQFDSLVLVNELGSRSYLIEEMGLTEDEATGFYRN
                     LEEYVRSGGNLILTDKALDALTEMGIVPQSAVSHGTFAPAPSFNFNVPNNGLTYEQAE
                     QYPLAAGLDLPGAAERNVGQRQGVEPAPLGYTPDSGDDATPAMPISVVDRAAWEVACG
                     KATPARCTTALGTTRTRTGTQTRNVTGVSLGELDLGEGRIRIAGNLFPAPAYQPDATN
                     DHRFGLASYALTYTGYIVFENLLKWTNPAKVDPAQLRATTLTFLSSDSSGYHSDDAPV
                     KLALTSQDGTPVAGAPVRIELTTAGEILESHRATTNGEGIAEIVLDLDEAPGEYGLVA
                     TFEEQEPFLGSVATASFLVRKELTRMVVGVSGTGADRLITARLTDDEGVPLAGRKVTF
                     WIDGVVVDQPAETNDSGVAKIAAPPGYQEGAHTYTAVFDADTTHEGSSGSAKSKG"
     gene            complement(7881..8102)
                     /locus_tag="VEV43_05520"
     CDS             complement(7881..8102)
                     /locus_tag="VEV43_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23013.1"
                     /translation="MLSLVALIGGAVSTAPAASADFKVCEVVGNVAPPAEDDCWGTTG
                     FIGDTWDRQADNVFFVFCEAFPGHEACQA"
     gene            complement(8217..8921)
                     /locus_tag="VEV43_05525"
     CDS             complement(8217..8921)
                     /locus_tag="VEV43_05525"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HYP23014.1"
                     /translation="MVQRLDGGRTADPYRDLDVVDARAPRFNQAAVALTTAASLLTGQ
                     WWIAAVMGVQLAVGLVFGRRYCLPCVFYFEVVQPRAGEGPVEDARPPRFANVLGAAFL
                     LGATAAHLAGLDTIGWTLIAMVAALATLAVVTGFCMGCSMYRLMSRLRGIRPGHHDRV
                     DTRDFGISGEAVVQFTHPLCSDCSELARSLERTGADVVLVDVTKSPALARKYHVDVVP
                     LALRVSVEGRVLERLA"
     gene            8948..>9176
                     /locus_tag="VEV43_05530"
     CDS             8948..>9176
                     /locus_tag="VEV43_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HYP23015.1"
                     /translation="MRRRVVVLGAVGLLVAIAAAWWFWWVPGWRPSLEPGERYGIDVS
                     AHQGPIDWERVAADGIEFAYVKATEGGDFVDE"
BASE COUNT         1451 a         3272 c         3103 g         1350 t
ORIGIN      
        1 tgacggctga cgtttcgtac ttcgcctgag caatcaggga tccgctatcc gtcccgatcg
       61 tgaggggaca gccggcgcgc gaggcggcgg ccctgcaaga gcgctggctc taagcgcagg
      121 tgctcaccgg tttcaatttc agaacgcccc aaccttggag cccctgcgag agcggggctc
      181 ccttgtttcg tggtgatgcg tccttacact gagtgcatgg agatttttcc ggggatcacg
      241 gtcgaccccg acgtgcggtt cggcaagccc tgcgtcacgg ggacgcgcgt cgacgtggcc
      301 acgatcgttg gagcgatcgg ggccggtgag cccaccggcg cggtcgccga ggagttcggg
      361 ctcaccctcg agcaggtcca ggccgccctt ctgtacgccg cccatgtcgc ggaccacgcg
      421 cccccggccg tccgcgaggt gtcttgaggg tcctcctcga cgagaacttc ccactccagc
      481 tccaaaggga gctgcgcttt cggggcatcg agtgcgacca catcatcgag ctcggcctca
      541 ggggtctgcc cgactcggaa atcctcgccc gcgtctcccg ggcaggacgg gatcgaacgc
      601 gtaccgctcg acctcatgtt ccgctccggc ttggccgcgg ccgtccccgt ctcgctcggc
      661 ctgtgggccg tctggcgaga gctcgaccgg caccgccggc caaggcactc ggctacttag
      721 ccgggacctt gtacgagatc ggccagtccg tgccgtccag gatgaacccc ggcggcgacg
      781 tgcgctcggc cgtgtgcatg atccagcacg gctgacacct cgtcgacccc gacgcgctcg
      841 gcgagatcag cgaccccgtc gacgctccga tgtccccgag cgggatcgtc acgctgaacg
      901 acttgctcgc cgggtcgaag gagccgtcct cgaacgcgac gagcttgcag tggctccacc
      961 cgaagcccgc gatctcggac ccctcctcac acgtctccag ccggaagtgg ccctcgaggt
     1021 cgacacggcc gacccggtcg gtgccctcct gcgtcagggc gtcgggcttg ccgacgtcct
     1081 ggctcttgat cgtgaagcgg tacctcgtgc cgttcgacat gaagttccag aagaacgtgg
     1141 cgccctcggg cagcgacccg gtcgcgggca tcgccgcgct cgtgaccgtg aactcgaggt
     1201 tgcgaccggc ccgggcgatc cggcccgaga cgaggtcgaa gcccgcctgg tcgaacccgg
     1261 ggatggagtt ctctcctacc gcggtcccgc cctcgatgcc ggcgtcgccg gcggggtctt
     1321 cgaagaccgt cgtcgggcgg ccggcactcg cggtgccgac gatgctgccg gcgaggcagg
     1381 cgccgaccat caccgtaaga agcttcttca cgagagtccc tttccgtccg gatcgtccag
     1441 cgactgagtt cgaccgggcg cccgggattc ctggcccgct cctcgtggga tggcgaagac
     1501 ctgtcagcgg cacggtccgt gaaaggagaa gaggccccac gagggggccc cttcactcca
     1561 tctgccgggc gcggctactg caaggctgcc tcggcggcgc gggtcatctg ctcccgtagc
     1621 tgctcgtcgc tgatgtcctg cgccatgacc tggatcgcat ggacgatcag ctcgtcgcgt
     1681 cgctcggccg ggatcacctg caggtcctgg cgaggtggga tcttgatgac cctcccactg
     1741 ggccgaaggt cgaagccccc gccatcgctg tcgccgccgc cgaacgagcc gatcgtggtc
     1801 aggatctcgt ccacgacgac cctcgtattc gtgtctccca tgtcgtcctc cttccgtccg
     1861 ctcgacgtgt cgatcgtgag ggagcccggc ggcgagcgct gtcagctagc taacgctccg
     1921 cctcgggacg gggttgggtc agtagcatga ccgggtgtcg aagttcgtcc cgggggtcga
     1981 gctctctcgt gcgttgtacg tcgaacacgt cgccgggctc ctcggcggcg tgtcacacgc
     2041 ggccgccctc ctcggtgagg gctcggaggt cctcggcttc gacaccgaac gatcgaccga
     2101 ccacgcctgg gggccgcggc tgcacgtctt cgtggcggcc gaggacgtag acgagacacg
     2161 ctcacgcata gacgggggac tgcccgacga cgtcctcgga tggcccactc gtgtctaccg
     2221 atggcagacg gacgaggtcg ctcatcacgt cgaggtcgag ccgctgggcc cctggctcga
     2281 gcgccagctc ggcttcgatc cccgcaacgg cgtcaccacg gcgcagtggc tcgcgacgcc
     2341 gcaacagagc ctcctccacg tcaccgcggg cccggtgttc gccgacgccg acggcgagct
     2401 gagcgcggtc agagaacggc tgagctggta tccgaaggac gtctggctat gggcgatggc
     2461 cgcggcgtgg cagcgggtgc aggacaacga gcacgtcgtc ggacgcaccg ccgagaccgg
     2521 cgacgacctc ggcaaccgcc tcgtcgtcgc gaagctcgtc gggcacctga cgtcgttgtg
     2581 cttcctccag gagaagcgct acgcgcctta cgtgaagtgg gtaggcgcgg cgttcgcgcg
     2641 cctcgacgcc gcagccgaga tcgggccgtc gttcgaccgg gccctctctt cgccggacgg
     2701 ggtggaacgg gaagatgcgg tcgcgagcgc gctcgaagcc gtagcgcgcc gacacaacga
     2761 cctgggcctc accgagcacc tcgatccggc gacgggaccc ttcaacgtga agatcaacga
     2821 cgcggtgcgg ccgttccgag tcctcaacgc gaaccgtttc gtgaacgcat gctcggccgc
     2881 gatcgaagac gaccaggtac gtgacgtagc gcccgtcggc gcgatcgacc agctcctcga
     2941 cccgaacgac ctgatcacca acttcacgga ttggccgcgc gagctggagg ccgtctacgc
     3001 gcgcaagctg ggggagtcct gaccgtgcgc cttcgcatcg ccggcatcgt cttgtccgtg
     3061 ctcctcggcg gtgcagccgt cgcggtgatc gtgctcgacg gcgaggagga gccgttcggg
     3121 cgcggggaca gcctgctgcc gagcttctcc gcgggctacc agtgcacgga cccgcgcgac
     3181 gagctcgcgg cggaggagct gtcgtacgtg ctcggctact cgcgggagca gtggtcggcg
     3241 cgcgtcgccc tgtcggtcgc cggaggcgag acgcggggac cgtgcagcgt ccagctcggc
     3301 gtgccaacgg gctcggaaga cctcgagtcc gaggacgcga acgtcttgtt ggagcgccac
     3361 ggctcgccgt cttccgggat cgcatcggtc ccactccggg accgtgcccg cgactacgtg
     3421 atgaacgtga cgctgccgcg cgacttcgag ctgttccacg cgctcggctt cggcaagtac
     3481 gtcttcgagc tcgacttctt cgcgccgggg gagggcgagt cgttcaagca ggcgacggtc
     3541 gacctgcgtc tccccgaggg ctactcgatc gacgaggcgc tcccgccagg cgacaacccc
     3601 ggctcggagc ggtcgaggaa gtggacgttc aaggcggacc gcgaccagca agcggtcgtc
     3661 acgttcgagc acgacaggtt gagaactgcc gtcgacctgg cccccgaggt tgccttcggc
     3721 gcccttctgt tggtgtagac gctcgtgacg ctgccgcggc gcagcgacga gagggccgag
     3781 ccggaaccgg cggtcgcgtc ccaacccatg ccgatcgaag cgacgcaacc ggagccggag
     3841 ccggaattgg ttgccgagcc cgaaccgact cccgagcccg agccggagcc gcttcccgag
     3901 cccgaaccga ctccggagcc cgagccggag ccgcttcccg agccggagcc gcttcccgag
     3961 cccgaacccc agccggtcgc cgctgcgatt ccggcgcccg agccgcagcc cgcgctcccg
     4021 gcccccgtcc cgccggcacc tccggccctt gccccgccgg cccccgcacc gcggccgctc
     4081 tcgcccgctc cgcggccccc ggcccccctt cccgaacggg tcccgagccg cacggcccgg
     4141 gccgccggag ccggggccgg gctcctcgcc gcggccctgt tcttccggct cgtccggcgg
     4201 ctccgcaacc gctgacccga cctccaaccc gggcaaatct ggacgaactc cgcgagcggc
     4261 catgtcccgt tccggccact ttgcggacac tttgcttggt ttgcaggaac cgcccccggc
     4321 gcgggcgaac ctccccccgc cgcaaccgac cgcggcgacc gaagaccggc acaaacagga
     4381 ggcacccatg tctgcccgcg cccgcctttc cgcggccgtc ctcgtcctct cgctcgtcgc
     4441 cacgctcgtc gcggcgatcg tcccgcgcga ggcgatggcc gtccccaacc ccgtcgccac
     4501 cgacgagccc acataccagc tgttcggccg ggtgttcccg gacccgcagg gctgcctggc
     4561 gcacgacgtc cccgacgaag acgtgaacgg catcaaggac acaccgaagg gcgtctcgcc
     4621 gtgggcgaaa ggccgcgtgt gcgccggtca gttcctctcg tacgaagagg tcatcgaggg
     4681 agcgaagttc atgtcgcgcc gcttcccgga cttcgtcgag gtcgtccgtc tcgaccaggc
     4741 gttcgacaac ccgaactatc gctcggccgg catcccgcgt tcgatctcgc tcgagaacgg
     4801 aaccgccaag gtcatggggc gcgaccgccg cccgctctac atgttcaggg tcacggaccg
     4861 cacgtcggac atccctccgc gcaagcgcga gcacttcgcc ttctcgctct cgatccacgg
     4921 ccccgagcgc gccgggctcg agggtggcat ccgcgcgatg gaggatctcg tgacgtgggc
     4981 cgcgtgcgag cggtccgact acgccgcgac gactcccgcc tgcgaagccg aaggggcgtt
     5041 tcccaagaag atcgtcgagt ccgcgaccga caggcccgtt cccacggccg gcgaggcgtt
     5101 gaagcggtcg gtcctgtact tcacgctgcc caaccccgac gggtggggcc gcggccagac
     5161 ggcaccggta gagatcgaag acggcggccc gaacgtcagc tacctcccgg gcatggcgtt
     5221 ccagcgcttc aacggcaacg gagtcgacct caaccgcgac tggccgacga tcggttacac
     5281 gctgaagtcg tactcgccgg gctccgagcc cgagacgaag gcgttcgcgg acgcgctgag
     5341 ccgcgtcaag gcgcgcacgg aggccgggag gttcgcgggc gggctcgacc tccacggcat
     5401 gctcaccgcg aaggccttca gctacacgct catcggcgcg ggccagcgcg actaccgcaa
     5461 gaacgcggtg acggtcgaga cgtcgctccg gacgtgggag gaccagacga acaggctgtc
     5521 gtggtcgccg tacgtcgcgg accacaacgc gaacggcgag aaggagcccg aggcaagccc
     5581 gcccgagatc tgccgtgaca cgaacggcac cgatgccacg ccgtgcgtcg cggaccagtg
     5641 gggcaccgtc atcgacacca tcggctacca agtcaccggc tcgttcggcg actggttcga
     5701 ctcaccgctc gggctcgacg cggtgggcgt gaacaacgag atgttcgtct cgcacgtcgc
     5761 accgaactcc gtgttcgagc ccgcgctcga gcagacccac atcgacggca acaagggcct
     5821 gatcttctcg cagatcgccg cgctgctccg gacgagcgag acgacgttcc accccggcgg
     5881 cacgaccgga tacgtcttcg acccgaaccg ccttcaggtc cccgcgcagc cacgcgtccc
     5941 gaaccccggc ctccccgcgc agaacgacat cgaggtcgtg ctgccgtgtc aggaccaggt
     6001 cgcgcagaac gcggacggct tctgcggcga cggagagttc gtcgtctccg gcacgcagac
     6061 gtcgtacgag ttcgacgtcg agggaccgcg gcacgagatc tggaacggcg ggatcacggc
     6121 gactacgacg cacgcgaacg ccaacggggt ctcgcccggg gcgctcccgg tcatccacct
     6181 cgattacttc gacgaaggac agtggcagcc ggtcgcacgc agcgtcgtcg gcgaggggca
     6241 cttctatcac caggccggca ggatcgtgac ggcgaacgac cccgagccgg gccggtggag
     6301 ggtccggttc ggcacgccgc tcgactcggt cacgcggctg caggtcgact tcaacccgta
     6361 caccgcggag gcttctccgg gccaggccgc gatcgacgcg tcgtcgatgg acttcttcag
     6421 cgagctgaac gagttcgtgc cggagggcga gaaggtcgcg ccggtcgacc cgaaggtcgt
     6481 gtaccggagc gtcctcgcgc tgcgccagtt cgattcgctc gtgctcgtca acgagctcgg
     6541 ctcccgctcg tacctgatcg aggagatggg actcacagag gacgaggcga cgggcttcta
     6601 ccgcaacctc gaggagtacg tccggtcggg cgggaacctg atcctcaccg acaaggcgct
     6661 ggacgccctc accgagatgg ggatcgtgcc gcagagcgcg gtgagccacg ggacgttcgc
     6721 tcccgctccg agcttcaact tcaacgtccc gaacaacggc ctcacgtacg agcaggcgga
     6781 gcagtatccg cttgccgcgg gactcgacct tccgggtgcc gcggagcgca acgtcggaca
     6841 gcggcagggt gtcgagccgg cgccgctcgg ctacacgcct gactccggtg acgacgcgac
     6901 gcccgcgatg ccgatctcgg tcgtggaccg cgccgcgtgg gaggtcgcgt gcggcaaggc
     6961 gacgccggcc cgctgcacga ctgcgctggg caccacacgc acgcgcaccg ggacccagac
     7021 caggaacgtc accggcgtga gcctcggcga gctcgacctc ggcgaaggcc gcatccgcat
     7081 cgccggcaac ctcttccccg ccccggcgta ccagccggac gcgaccaacg accatcgctt
     7141 cgggctcgcg tcgtacgctc tcacctacac cggctacatc gtgttcgaga acctgctgaa
     7201 gtggaccaac ccggcgaagg tcgacccggc gcagctacgc gccaccacgc tcacgttcct
     7261 ttcttcggac tcgtcgggct accactccga cgacgcgccg gtgaagctgg cgctgacgtc
     7321 gcaggacggg accccggtcg cgggcgcccc ggtccggatc gagctgacga ccgccggcga
     7381 gatcctcgag agccaccgcg cgacgacgaa cggagagggg atcgcggaga tcgtcctcga
     7441 cctcgacgag gcccccggtg agtacgggct ggtcgcgacc ttcgaggaac aggagccctt
     7501 cctagggtcg gtcgcgacgg cgtcgttcct ggtccgcaag gagctgaccc ggatggtcgt
     7561 cggcgtgtcc ggcacgggcg ccgaccgcct gatcactgcc cgcttgacgg acgacgaggg
     7621 tgttccgctg gcggggagga aggtgacgtt ctggatcgac ggggtcgtgg tcgaccaacc
     7681 cgccgagacg aacgacagcg gggtagcgaa gatcgccgcg ccgccggggt atcaggaagg
     7741 tgcgcacacg tacaccgcag tcttcgacgc ggacacgacg cacgaaggca gcagcggaag
     7801 cgcgaagagc aagggctgag gccaaagaga ggggcccgct cgggcccctc tctctttttc
     7861 cgtccgcctc tcggctcgag ctaggcctga caggcctcgt ggcccgggaa cgcctcacag
     7921 aacacgaaga agacgttgtc tgcctgccgg tcccaggtgt cacctatgaa tccggttgtc
     7981 ccccagcagt cgtcctcggc gggcggcgcc acgttgccca cgacctcgca caccttgaag
     8041 tctgcggacg cggccggcgc ggtcgatacc gcacctccga tgagagcgac aagagacaac
     8101 acgaccgtgg atactcggcg catccggtac ctccccaggt ttgggcttcc tcgctgaagc
     8161 cgtcgagggg acggtttcgg gaggcgggcg agcctctcct ccccgcggcc gagaatctag
     8221 gcgagacgct ccagcacgcg gccctcgacc gagacccgca gagccagcgg cacgacgtcc
     8281 acgtggtact tgcgggcgag cgccgggctc ttcgtgacgt cgacgaggac tacgtcggcg
     8341 ccggtccgtt cgagcgagcg cgccagctcc gagcagtcgc tgcacagcgg atgcgtgaac
     8401 tgcacgaccg cctctccgga gatcccgaag tcgcgcgtgt cgacgcggtc gtggtggccg
     8461 ggacggatgc cgcgcagccg ggacatcagc cggtacatgc tgcagcccat gcagaagccg
     8521 gtgacgacgg cgagcgtcgc aagcgccgcg accatcgcga tcaacgtcca cccgatcgtg
     8581 tcgagccccg cgagatgagc cgccgtcgcc ccgagcagga acgccgcccc caggacgttc
     8641 gcgaagcgcg ggggccgcgc gtcctcgacc gggccctcgc cggcgcgcgg ttgcacgacc
     8701 tcgaagtaga agacacacgg caggcagtac ctgcggccga agacgagccc gaccgcgagc
     8761 tgcactccca tgacggcggc gatccaccac tgcccggtga gaagcgaggc cgcggtcgtg
     8821 agggcgacgg ccgcctggtt gaaccgcggc gcccgggcgt cgacgacgtc gagatccctg
     8881 taggggtcgg cggtccggcc cccgtccagc ctttgcacca tttgcacggg tgaaatagta
     8941 tcaccgcgtg cggcgtcggg tcgtggtctt gggagccgtg gggctcctcg tggccatcgc
     9001 cgcggcgtgg tggttctggt gggtccctgg ctggcggccg tcgctggagc ccggcgagcg
     9061 atacggcatc gacgtctccg cgcaccaggg cccgatcgac tgggaacgag tggccgcgga
     9121 cgggatcgag ttcgcgtacg tcaaggcgac cgaaggcggc gacttcgtcg acgaac
//
LOCUS       DASQDO010000191         5962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1060547, whole genome shotgun sequence.
ACCESSION   DASQDO010000191 DASQDO010000000
VERSION     DASQDO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060547"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VEV43_05535"
     CDS             <1..899
                     /locus_tag="VEV43_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYP23016.1"
                     /translation="ATGMPYLRPPKGARDLSVLRVAGSMGYRSVRWTQYPKGGLAKKI
                     AYKVVSRVQPGSIISLDVWRESHRKALPKIIDRLRKRNFNLRQIDALANTHAVRWDVT
                     LNAGDSGAEVSYLQKTLDEISYPAGRRDGSFGYETQQAVYAFEKVKGLTRDGVVTPQQ
                     MTQIALSTRPVVPSKGKPKTFVDVDISRQVLFEVKNGRVTHTLPISSGNEEYYTVDGE
                     TYKAHTPRGSFTIERKIEGVRVSRLGKLYHPSYFVGGYAIHGSQSVPVYPASHGCVRI
                     PMYVRKAFFRRTPVGKAVFVHN"
     gene            complement(939..1967)
                     /locus_tag="VEV43_05540"
     CDS             complement(939..1967)
                     /locus_tag="VEV43_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYP23017.1"
                     /translation="MNKRLAPLFVLLSLAGSLHAGPAQAALKNVPDWAVPAVRYLAGN
                     GALDAGDFKPNEPLKRSAFKKVMAKTFGGGYTRTKGYVTAAEVSAALVRALGKAPIAS
                     RLRNAKSPDGWQPEVDRWFGSEIVAREMGLRRDRATSEEAREASAGEPMRGADVVYAV
                     WKAKTSPSTWGADALAGFSLSSYGPVRREVVEYALSLVGTPYVWGGEWVRKTPAGYPY
                     GAQTHGGVDCSGFVWYVLRKKAPGWAPTGRPYAGWSFPERSSAHMAAATEKRLGYRQL
                     QAGDVVFFAPNGRRSKASDVYHAGLYLGRGWMIHSSGSRAGVSLASIAPGAWWHDQIA
                     WGRRVIRS"
     gene            2102..3547
                     /locus_tag="VEV43_05545"
     CDS             2102..3547
                     /locus_tag="VEV43_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23018.1"
                     /translation="MRRTKLLLVPALLASLVPLTGAAVSAPVRCPSVKPAPLDFGKPI
                     FIDKTRAGGEPVSIIADDGSIIVSAHAGTTHVYKDPNALPGAGDFAGSYYNQTLNWRS
                     TDGGKTWQYVGLAGAPAGPHSPTSTGFSDPDLTKDAAGNIYNVEINLANVSVFGSPDD
                     GQSWPTANPVAASGDRPWVTGQEEDEVFLYVNLPKSLWRSTDGGLTFTLVGQGGDVGP
                     DSKMITDPINPKSGLLGPINAGGVSISKDDGVTWTDYPAPLGDSTQFFGAIAADRAGW
                     IYAANAGGYTGSSDTTPDGTVTFNYFNRKTKRWAAQPIEVPTPKGDALWPWIIAGDDG
                     RVAITWYQTLAGKPDAFRAYVAYTTNGHGSRVKCSDGSTKFIPPQFQVADAAGRPIHK
                     GKICLSGTTCNANPSFEGGDRRLGDFFTVNFDHKGTLFIVSGDTTLPSPTGGPKPVAN
                     PIFVKQTSGDKMLRKPDRTRPTRCLFPLPTC"
     gene            3565..4284
                     /locus_tag="VEV43_05550"
     CDS             3565..4284
                     /locus_tag="VEV43_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23019.1"
                     /translation="MRGCGLAGIGVALLASIAAVVPLSSLARGQDAAPEVRMEASAAV
                     VTWGDAIRFTGTVSSPEAPECHAGVEVMLEMDAHDDRTHWTEVARARTDENGAFVADV
                     RAEEPANYRMTTVPEEGSGCDAGVAAGGEVMVRFKVTLERSSLVVDKGEEVRLTVRVE
                     PFCPVGGPSFDVRKIPLYELRGERFVRVAAKRDANDCTVTFTRKVRGLSVFMSKVRDN
                     EELNARHLGGRSAEVAVTVRN"
     gene            complement(4289..5338)
                     /locus_tag="VEV43_05555"
     CDS             complement(4289..5338)
                     /locus_tag="VEV43_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HYP23020.1"
                     /translation="MPRLRKADCSGPGLTRRRRGKGFEYLDEHGRRITDPEVLSRVRN
                     LVIPPAWSDVWICPLPNGHIQAIGTDAAGRKQYMYHEDWRRRRDAEKFEHMLDFAAAL
                     PGMRRRCMKDLRRDGYPKERVLACAVRLLDRGFFRIGTEAYAEQNESYGLATMQKRHV
                     TVSGDVITFDYVAKSGKHRIQSLVDTEVLEVVKALKRRRAGGPELLAYKDGAGWCDVK
                     SADINAYVKRITGGDFTAKDFRTWSATVLAAVALAVSGEAAKSRTARNRAMSRAVQEV
                     SHYLGNTPAVCRASYIDPRVFDRYLSGWTISGALEQLGEDQDFGEPSTQGAVEAAVLE
                     LLANNKSSDALEKIA"
     gene            5400..>5962
                     /locus_tag="VEV43_05560"
     CDS             5400..>5962
                     /locus_tag="VEV43_05560"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HYP23021.1"
                     /translation="MSQAARTGEAPDRNLALELVRVTEAAALSAARWMGRGDKEAADQ
                     AAVDAMRLLMDTVPMDGVVVIGEGEKDNAPMLYNGERIGNGEPPVVDIAVDPIDGTTL
                     TSKGMNGALAVVALAERGTMFDPGPCVYMEKIAAGEEAADAIDIEAPVAENLKRVAKA
                     KHADVSDVTVTILERSRHEQLVKEVREA"
BASE COUNT          953 a         2121 c         2022 g          866 t
ORIGIN      
        1 gcgccaccgg catgccttac ctccggcccc cgaagggcgc acgcgacctg agcgtcctgc
       61 gcgtcgccgg ctcgatgggc taccgctccg tccggtggac gcagtacccg aagggcgggc
      121 tcgccaagaa gatcgcctac aaggtcgtgt cgcgtgtcca gcccggctcg atcatctccc
      181 tcgacgtgtg gcgtgagagc caccgcaagg cgctcccgaa gatcatcgac cggctgcgca
      241 agcgcaactt caacctgcgg cagatcgacg cgctcgcgaa cactcacgcc gtgcggtggg
      301 acgtgacgct gaacgcgggg gactcggggg ccgaggtctc gtacctgcag aagaccctcg
      361 acgagatctc gtacccggcc ggccgccgcg acgggtcgtt cggctacgag acacagcagg
      421 ccgtctacgc gttcgagaag gtcaaggggc tcacgcgcga cggcgtcgtc acgccgcagc
      481 agatgacgca gatcgcgctg tcgacccggc ccgtggtgcc gagcaagggc aagccgaaga
      541 cgttcgtcga cgtcgacatc tcacggcagg tgctgttcga ggtgaagaac ggccgcgtca
      601 cgcacacgct gccgatctcg agcgggaacg aggagtacta cacggtcgac ggagagacct
      661 acaaggcgca cacgccgcgc ggcagcttca cgatcgagcg caagatcgaa ggcgtacgcg
      721 tgagccggct cggcaagctc taccacccga gctacttcgt cgggggctac gcgatccacg
      781 ggagccagtc ggtgcccgtt tatccggcga gccacggctg cgtccggatc ccgatgtacg
      841 tgcgcaaggc cttcttccgc cgcacgccgg tcggcaaggc ggtcttcgta cacaactagc
      901 ggcggcctcg gacggcgcgc cgaacggaag tgctcaggct agctgcggat gaccctccgg
      961 ccccacgcga tctggtcgtg ccaccaggcg cccggggcga tcgacgcgag gctgactccg
     1021 gcgcgcgatc ccgacgagtg gatcatccag ccgcggccga ggtagagacc tgcgtggtac
     1081 acgtccgacg ccttcgacct gcgtccgttc ggcgcgaaga agaccacgtc gcccgcctgt
     1141 agctgccggt agccgagcct cttctcggtc gcggccgcca tgtgcgcgga cgagcgctcg
     1201 gggaacgacc accccgcgta ggggcgaccg gtgggtgccc atcccggcgc cttcttgcgc
     1261 aggacgtacc agacgaagcc ggagcagtcg acgcctccgt gcgtctgcgc gccgtacggg
     1321 tatcccgcgg gggtcttgcg gacccactcg ccgccccaga cgtacggcgt cccgacgagc
     1381 gagagcgcgt actcgacgac ctcgcgcctc acggggccgt agctcgacag cgagaagccc
     1441 gcgagcgcgt cggcgcccca ggtcgacggt gacgtcttgg ccttccagac cgcgtagacg
     1501 acgtccgccc cgcgcatggg ctcgcccgcg gacgcctcgc gcgcctcttc ggacgtggcc
     1561 cggtcccgcc ggagccccat ctcgcgggcg acgatctcgg acccgaacca cctgtcgacc
     1621 tcgggctgcc agccgtcggg cgacttcgcg ttcctaaggc gagaggcgat cggtgccttc
     1681 ccgagcgccc gcacgagcgc ggccgacacc tccgccgcgg tgacataccc cttcgtgcgc
     1741 gtgtacccgc cgccgaaggt cttcgccatg accttcttga aggcgctgcg cttcagcggc
     1801 tcgttcggct tgaagtcacc ggcgtcgagg gccccgttgc cggcgaggta gcggacggcc
     1861 ggcacggccc agtccgggac gttcttcagc gccgcctgtg cggggcccgc gtggagggag
     1921 ccggccagcg acaacaggac gaaaaggggc gcaagtctct tgttcacccc ccacgtatcg
     1981 gaagaaaatg ggcaagatct gaatccccgg ccggggtggg cctcggcccc cgggtggggg
     2041 aggaaccagg gctagcgggg cgaatctccc aacgaccagt cctagaccgg gagtcctcgt
     2101 catgcgccga acgaagttgt tgttggtccc cgctctgctc gcgagcctgg ttccgctcac
     2161 cggggcggcc gtgtcggcgc ccgtccggtg cccctcggtc aagcccgccc cgctggactt
     2221 cggcaagccg atcttcatcg acaagacccg ggccggaggc gagccggtca gcatcatcgc
     2281 cgacgacggc tcgatcatcg tgtcggctca cgccgggacg acccacgtct acaaggaccc
     2341 gaacgcgctg cccggcgcgg gtgacttcgc cggcagctac tacaaccaga ctctgaactg
     2401 gcgctcgacc gacggcggca agacgtggca gtacgtcgga ctcgccggcg ctcccgccgg
     2461 gccgcacagc ccgacctcga ccgggttctc ggaccccgac ctcaccaagg acgcggccgg
     2521 gaacatctac aacgtcgaga tcaacctcgc caacgtctcg gtcttcggga gccccgacga
     2581 cggccagtcg tggccgaccg cgaaccccgt cgccgcgtcc ggcgaccgtc cctgggtgac
     2641 cggccaggag gaggacgagg tcttcctcta cgtcaacctg ccgaagtcgc tgtggcgctc
     2701 gaccgacggc gggctcacgt tcacgctcgt cggccagggc ggcgacgtcg gcccggacag
     2761 caagatgatc accgacccga tcaacccgaa gtccggtctg ctcggcccga tcaacgccgg
     2821 cggcgtctcg atctcgaagg acgacggcgt cacgtggacc gactacccgg ccccgctcgg
     2881 cgactcgacc cagttcttcg gcgcgatcgc cgccgaccgc gccgggtgga tctacgcggc
     2941 gaacgccggc gggtacaccg gctcgagcga cacgacgccg gacggcacgg tcacgttcaa
     3001 ctacttcaac cggaagacga agaggtgggc cgcgcagccg atcgaggtgc cgacgccgaa
     3061 gggcgacgcg ctgtggccgt ggatcatcgc cggtgacgac ggccgggtcg cgatcacgtg
     3121 gtaccagacg ctcgcgggca agcccgacgc gttccgcgcc tacgtcgcct acacgacgaa
     3181 cggccacggc tcccgcgtga agtgctccga cggctcgacg aagttcatcc cgccgcagtt
     3241 ccaggtcgcg gacgcggccg gccgcccgat ccacaagggc aagatctgcc tgagcgggac
     3301 gacctgcaac gcgaacccga gcttcgaggg cggcgaccgc cgcctcggcg acttcttcac
     3361 ggtgaacttc gaccacaagg gcacgctgtt catcgtgtcg ggcgacacga cgctgccgag
     3421 cccgacgggg gggccgaagc cggtcgcgaa cccgatcttc gtgaagcaga cgtcggggga
     3481 caagatgctg cgcaagccgg accgcacccg cccgacgcgc tgcctcttcc cgctgccgac
     3541 ctgctagccg tactccgaag caccgtgcgt ggttgcggcc tagccggcat cggcgttgcc
     3601 ctgctcgctt cgatcgccgc cgtggtgcct ctctcgtcgt tggcgcgcgg gcaggacgcg
     3661 gcacccgagg tgcggatgga agcgtccgct gcggtcgtta cgtggggcga cgcgatccgg
     3721 ttcaccggaa cggtgtcctc gcccgaagcc cccgagtgcc acgcgggcgt cgaggtgatg
     3781 ctcgagatgg acgcccatga cgaccgtacg cactggaccg aggtcgcccg agccaggact
     3841 gacgagaacg gggcttttgt cgcggacgtg agggcggagg agccggctaa ctaccgcatg
     3901 accaccgtcc ctgaggaggg cagcggctgc gatgccgggg tggcggccgg cggtgaggtg
     3961 atggtcagat tcaaggtcac actcgagcgc agctccctag tcgtcgacaa aggcgaggag
     4021 gtccgtctga cggtccgcgt cgagcccttc tgtcccgtcg ggggcccgtc cttcgacgtt
     4081 cggaagattc cgctgtacga gttgcgcggc gaaaggttcg tccgggttgc cgccaagcgg
     4141 gacgcgaacg actgcaccgt cacgttcact cggaaggtcc gcggactgag cgtcttcatg
     4201 tcgaaggtgc gtgacaacga ggagctgaat gcgaggcacc tcggcggccg ctcggccgag
     4261 gtcgccgtca cggtccgcaa ctgacctgct aggcgatctt ctccagcgcg tccgagctct
     4321 tgttgttcgc cagcagctcc aggaccgcgg cctctaccgc gccctgcgtc gaaggctccc
     4381 cgaagtcctg gtcctcgccg agctgctcga gcgcgccgct gatcgtccag cccgacagat
     4441 agcggtcgaa cacgcgcgga tcgatgtagg acgcgcggca gaccgccggc gtgttgccga
     4501 ggtagtggga cacctcctgg acggctcggg acatcgcgcg gttgcgtgcc gtgcgtgact
     4561 tcgcagcctc gcccgacacc gcgagcgcca cggcggcgag cacggtcgcg ctccaggtgc
     4621 ggaagtcctt ggccgtgaag tcgccgccgg ttatccgctt cacgtaggcg ttgatgtcgg
     4681 cggacttcac gtcgcaccag ccggcgccgt ccttgtaggc gagcagctcc ggcccccccg
     4741 cccgcctccg cttgagcgcc ttcacgacct cgagcacctc ggtgtcgacg agcgactgga
     4801 tccggtgctt gccgctcttg gcgacgtagt cgaacgtgat cacgtcgccg gagaccgtga
     4861 cgtggcgctt ctgcatcgtc gcgagcccgt acgactcgtt ctgctccgcg tacgcctccg
     4921 tcccgatccg gaagaaaccg cggtcgagca accggaccgc gcacgcgagc acgcgctcct
     4981 tcgggtagcc gtcgcggcgg agatccttca tgcagcgcct ccgcatcccc ggcagtgccg
     5041 cggcgaagtc cagcatgtgc tcgaacttct cggcgtcccg ccgcctgcgc cagtcctcgt
     5101 ggtacatgta ctgcttccgc ccggctgcgt cggtgccgat ggcctggatg tggccgttcg
     5161 gcagcggaca gatccagacg tcgctccacg ccggcgggat gacgaggttc cgcacgcgcg
     5221 acaggacctc gggatcggtg atccgtctcc cgtgttcgtc gaggtactcg aaccccttgc
     5281 cccggcgccg tctcgtgagc ccggggcccg aacagtctgc tttcctcagg cgcggcaccc
     5341 cgggatgttc cccggtaatc tcgacgccca acccccgccc ccccgacgag gaggaccgca
     5401 tgtcccaagc cgcccgtacg ggcgaggctc ccgaccggaa cctggcgctg gagctcgtcc
     5461 gcgtgaccga ggccgcagcg ctctcggccg cccggtggat ggggcgcggc gacaaggagg
     5521 cggcggacca ggcggcggtc gacgcgatgc gcctcctcat ggacacggtc ccgatggacg
     5581 gcgtcgtcgt catcggcgag ggcgagaagg acaacgcgcc gatgctctac aacggcgagc
     5641 gcatcggcaa cggcgagcct cccgtcgtcg acatcgcagt cgacccgatc gacggcacga
     5701 cgctcacgtc caaggggatg aacggagcac tcgcggtcgt cgcgctcgcc gagcgcggga
     5761 cgatgttcga ccccggcccc tgcgtctaca tggagaagat cgcggccggc gaggaggctg
     5821 ccgacgcgat cgacatcgag gcccccgtcg ccgagaacct gaagcgcgtg gcgaaggcca
     5881 agcacgcgga cgtgtcggac gtgaccgtca cgatcctcga gcgctcgcgc cacgagcagc
     5941 tcgtgaagga ggtccgcgag gc
//
LOCUS       DASQDO010000192         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_448301, whole genome shotgun sequence.
ACCESSION   DASQDO010000192 DASQDO010000000
VERSION     DASQDO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448301"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VEV43_05565"
     CDS             <1..200
                     /locus_tag="VEV43_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HYP23022.1"
                     /translation="MLSGDRWVHLWLCLECGHVGCCDSSPNRHATKHHHATDHPLIQS
                     FEPGEDWIYCYVDDVAMRSSP"
     gene            214..1107
                     /locus_tag="VEV43_05570"
     CDS             214..1107
                     /locus_tag="VEV43_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HYP23023.1"
                     /translation="MFRLSEYRDLVELDRPFVPAAAPAPPEELPSLTRRALGLLSEGP
                     LPATVDPRAALRDLLTLRQPSPLPPDAAPVLDALLGGERELRPRVDPKRLPSVAEDFP
                     GTSHAAAATTALWRGDVTTLAADAVVNAANSALLGCFLPNHPCIDNAIHAAAGPHLRA
                     DCAEIVRAQGAAEPTGSAKVTRGYHLPARYVLHTVGPIVHGEPGDDDAAALASCYRSC
                     LDLAASLEDVRTVAFCSISTGVFGYPKGAAARVALATVAEWFDAHPGRLERVVFDVFT
                     ADDLDVYTRAFSEDDRDESGT"
     gene            1288..2580
                     /locus_tag="VEV43_05575"
     CDS             1288..2580
                     /locus_tag="VEV43_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP23024.1"
                     /translation="MPRSLFTLVPQLPSRAWKVLAGDSIAAIGTGLVLPFMVVYLRDV
                     RGFHVQTAALVLSLLAVTGLVFGAPFGALIDKLGPRKTLIASLLLCAVSSATFSQVTE
                     PWQAFAAAFFLGIGFAGMWPSTHSLLSSVVGAEHRSSVFSVHYATLNLGIGIGGIAGG
                     LLANVSNEWTFELLYGLDALSWLAFALVLLKMSDIGNRVERSDEDKEIAAGGYRAVLR
                     DRNFIRLFVLMTLLVTIGYAQLESGFPAYATGEAGISTTALGMTFAANTFSIVLAQLV
                     VLKLMDGKRRTRGLMGICVLWAIAWSLVVVAGQLDDGLLKNAGFALVMVVFGLGECLV
                     SPTVPAMLNDLAPDALRGRYNAAYSFTFSAGHIVGPALAGAFLGAGRGEAWFAVLAVA
                     SIAAIALVRNLERHVPERANRVTEEAPPAPAEPELAPA"
     gene            2675..>3713
                     /locus_tag="VEV43_05580"
     CDS             2675..>3713
                     /locus_tag="VEV43_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23025.1"
                     /translation="MRWRGRASARATNVAMAAALLLGLLVAVAPVAAGAEPNVPRCPS
                     RMTSQPLFQGDVPPTDCVGSRLLEFDPPTIPAIAFDEVLRFTWPEMCTNEQTQTSPSK
                     CIWRWKVQLIFFEERPDGSLQQLYPQSFRFEFATDDPGCDDIRNYCEFIVRPDATVPD
                     DVVVVAGVYATSVFCNPPGGFAGCDAEDRPWITVAQNVGEIVEPPGGLDVSLTALSPS
                     GDHPLDGVISVAETTTVRLTLTNEGDRPLTDFTFAGGSPLVVDARSTGGLEIVSGPAP
                     PVDPALALAPGEAKTFTYEVEATAEGVAAAHSKVTAFDPDLVQHSEAHSLRFDIADGI
                     RMTREVGEYLRL"
BASE COUNT          501 a         1333 c         1266 g          613 t
ORIGIN      
        1 gcatgctgtc gggcgaccgc tgggtccacc tctggctgtg cctcgagtgc ggccacgtgg
       61 gttgctgtga cagctcgccg aaccgccacg cgacgaagca ccaccacgcg acggatcacc
      121 cgctgatcca gtcgttcgag cccggcgagg actggatcta ctgctacgtc gacgacgtcg
      181 cgatgcggtc ttctccctag cgtcgaaccc agcgtgttcc ggctgtccga gtaccgcgac
      241 ctggtcgagc tcgaccgccc gttcgttccg gcagcggctc ccgcgccgcc ggaggagctg
      301 ccgtccctca cgcggcgcgc tctcgggctc ctgtccgaag gtcccttgcc ggcgacggtc
      361 gatccccgtg ccgcgctgcg ggacctgctg acgctgcggc agccttcgcc cctgcccccg
      421 gacgcggccc ccgtcctcga cgcgctgctc ggcggcgagc gcgagctccg gccgcgcgtc
      481 gatccgaagc ggcttccgtc cgttgccgag gacttccccg ggacttctca tgcggcggcg
      541 gccacgaccg ccctctggcg cggggacgtc acgacgctcg cagcggacgc cgtcgtcaac
      601 gccgccaaca gcgcgctgct cggctgcttc ctcccgaacc acccctgtat cgacaacgcc
      661 atccacgcgg cggccgggcc gcaccttcga gccgactgcg ccgagatcgt gcgcgcgcag
      721 ggcgcggccg agccgacggg gtcggcgaag gtcacccgcg gctaccacct ccccgcgcgc
      781 tacgtcctgc acacggtcgg cccgatcgtg cacggcgagc ccggcgacga cgacgccgcg
      841 gcgctggcgt cctgctaccg ctcgtgcctg gacctggccg cttcgctcga agacgtccgc
      901 acggttgcgt tctgctcgat cagcacgggt gtcttcggat accccaaggg ggccgcggcc
      961 cgcgtcgcgc tcgcgacggt ggcggaatgg ttcgacgcgc accccggccg cctcgagcgc
     1021 gtcgtgttcg acgtcttcac ggccgacgac ctcgacgtct acacacgcgc cttctcagag
     1081 gatgaccggg acgaatcggg cacgtagttg acgaggcctc gccggttcgg gtttcatagc
     1141 gacttcatga agccgaccgg acgcaacttc cacacgggtg tccccggggc cgttccagcg
     1201 gccacggcct gctgcgctac ccgatagcgc ccccgtcgcc gtagggcgac gacccgtccg
     1261 tccgagcttc gaccaaaggt ccacgtcatg cccagatccc ttttcaccct cgttccgcaa
     1321 ctcccgtccc gcgcctggaa ggtgctcgcc ggcgactcca tcgcggcgat cggcaccggg
     1381 ctcgtcctgc cgttcatggt cgtgtacctg cgcgacgtca ggggcttcca cgtccagacc
     1441 gcggcgctcg tcctgtcgct cctcgcggta acagggctgg tcttcggcgc gcccttcggg
     1501 gcgctgatcg acaagctcgg cccccgcaag accctcatcg cgtcgctgct gctgtgtgcg
     1561 gtgtcgtcgg cgacgttctc tcaggtcacc gagccgtggc aggcgttcgc cgcggcgttc
     1621 ttcctcggca tcgggttcgc ggggatgtgg ccgtcgaccc actcgctgct ctcgtcggtc
     1681 gtgggggccg agcaccggtc gagcgtcttc tcggtccact acgcgacgtt gaacctcggc
     1741 atcgggatcg gcggcatcgc cggaggtctg ctcgcgaacg tctcgaacga gtggacgttc
     1801 gagctcctct acgggctcga cgcgctgtcg tggctcgcgt tcgcgctcgt gctcttgaag
     1861 atgagcgaca tcgggaacag ggtcgagcgc tccgacgagg acaaagagat cgccgccggc
     1921 ggctaccgcg cggtgttgcg cgaccggaac ttcatccggc tgttcgtcct gatgacgctg
     1981 ctcgtcacga tcgggtacgc gcagctcgag tcgggcttcc ccgcgtacgc cacgggcgag
     2041 gccggcatct cgacgaccgc gctcggcatg acgttcgcag ccaacacgtt ctcgatcgtg
     2101 ctggcgcagc tcgtggtgct gaagctcatg gacggaaagc gccgcacgcg cgggctcatg
     2161 gggatctgcg tcctgtgggc gatcgcgtgg agcctcgtgg tcgtcgcggg gcaactcgac
     2221 gacggactgc tgaagaacgc agggttcgcg ctggtgatgg tcgtcttcgg cctcggcgaa
     2281 tgcctcgtct cgccgacggt cccggcgatg ctgaacgacc tcgcgccgga tgcgttgcgg
     2341 ggccgctaca acgcggcgta ctcgttcacg ttctcggccg ggcacatcgt cgggcccgcg
     2401 ctggccggag cgttcctggg cgccggccgg ggcgaggcgt ggttcgccgt gctggcggtg
     2461 gcgagcatcg ccgcgatcgc gctggtgcgg aacctcgagc ggcacgtgcc ggagagggcc
     2521 aatcgggtga ccgaggaagc ccctccggca cccgccgagc ccgagctcgc gcccgcctga
     2581 ccatcccgca gcaccacccg gccggactat tcacacccgg cggcgactag cccaggatgt
     2641 gctcgaggcg gcgacacgaa cggaagggtg ctgcatgagg tggaggggcc gggcgtcggc
     2701 gcgcgcaacg aacgtggcga tggcggctgc cctgttgctc ggattgctcg tcgccgtcgc
     2761 cccggttgcg gccggcgcag agccgaacgt cccgcgctgt ccctcgcgca tgacgagcca
     2821 gcccctgttc caaggtgacg taccgccgac cgattgcgtc gggtcgcgcc tgttggagtt
     2881 cgaccccccg acgattccgg cgatcgcgtt cgacgaggtc ctgcggttca cgtggcccga
     2941 gatgtgcacg aacgagcaga cgcagaccag cccgtccaag tgcatctggc gctggaaggt
     3001 gcagctgatc ttcttcgaag agaggcccga cgggtcgttg cagcagctgt acccgcagtc
     3061 gttcaggttc gagttcgcaa ccgacgaccc cgggtgtgac gacatccgga actactgcga
     3121 gttcatcgtg cgccccgacg ccacggttcc cgacgacgtc gtcgtggtcg ccggcgtgta
     3181 cgcgacctcg gtcttctgca acccgccggg cgggttcgcc ggctgtgacg cggaggatcg
     3241 tccgtggatc accgtcgcgc agaacgtcgg tgagatcgtc gagccccccg gtgggctgga
     3301 cgtctcgcta accgcgctgt cgccgtccgg cgaccatccg ctcgacggcg tcatctcggt
     3361 cgcggagacg acgacggtca ggctcacgct cacgaacgag ggtgatcgcc cgctgacgga
     3421 cttcacgttc gccggcgggt cgccgctggt cgtcgacgcg cgcagcaccg gcgggttgga
     3481 gatcgtctcg gggcctgctc cgcccgtcga tccggcgctg gcgctcgcgc cgggcgaggc
     3541 gaagacgttc acgtacgagg tcgaggcgac ggcggaagga gtcgccgccg cgcactcgaa
     3601 ggtcacggcg ttcgaccccg acctcgtcca gcactccgaa gcacattcgc tgcggttcga
     3661 catcgcggac ggcatccgga tgacgcgcga ggtcggcgag tatctccggc tcc
//
LOCUS       DASQDO010000193         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_142749, whole genome shotgun sequence.
ACCESSION   DASQDO010000193 DASQDO010000000
VERSION     DASQDO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142749"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VEV43_05585"
     CDS             <1..811
                     /locus_tag="VEV43_05585"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HYP23026.1"
                     /translation="LAAFYPSAGGRAAEGAEEVFLATVAEHEEALRVDTARPLQTNEP
                     GRAGALVGGFLTVAERFGLPLRVLEVGASAGLNLRWDRFLYEARGATWGDPGSPVRLC
                     DFNSESPPPFGVDATVAERRGCDPSPIDPATDDGATLLLSFVWPDQLSRIRLLRGAIE
                     VARKVPAAVDEEPASSWLRSLARPRDGLATVVFHSIVHQYLGDEEAVAVTDALEAAAG
                     AATATAPFAYLTMEPGDDEKVVEVRLTTWPGAAEELLAHSGYHGTAVRWLG"
     gene            complement(808..1332)
                     /locus_tag="VEV43_05590"
     CDS             complement(808..1332)
                     /locus_tag="VEV43_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23027.1"
                     /translation="MRNRLLRVSAVAVMLSCLWAAPALAVEQQDPADTGLKLDISLIK
                     FVEDPEDVATLTIKTHSGWKCGALRPAAKTSLKWFFDGSGNNDFDLVGSFVCRGGTLV
                     FNLRSTDGGSQYEPLVAKKPNRRTVRVTMTLDLPQLDSNNLDLIAKSKDTSGETCIED
                     CVDRAPDEGRMKAY"
     gene            1381..>1611
                     /locus_tag="VEV43_05595"
     CDS             1381..>1611
                     /locus_tag="VEV43_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23028.1"
                     /translation="MTGGSRPYAPLGVAIGVFVVLVVVLLLVGGLGAQSPPNEHREPA
                     TDGRGDVSIGVSWSPPFEKFDTCVISPFEPGAE"
BASE COUNT          218 a          566 c          584 g          243 t
ORIGIN      
        1 gcttgcggcc ttctatccgt cggccggggg ccgggccgcc gagggggccg aggaggtctt
       61 cctcgcgacg gtcgccgagc acgaggaggc gctgcgcgtc gacaccgccc ggccgctgca
      121 gacgaacgag ccgggccggg cgggggcgct cgtcggcggc ttcctcacgg tcgcggagcg
      181 cttcgggctg ccgctccggg tgctcgaggt cggcgccagc gcggggctca acctgaggtg
      241 ggaccggttc ctctacgagg cgcggggcgc gacctggggc gacccgggct cgcccgtgcg
      301 gctgtgcgac ttcaacagcg aatcgccgcc tccgttcggc gtcgacgcga cggtggcgga
      361 gcggcgcggc tgcgacccct cccccatcga cccggcgaca gacgacggcg cgacgctgct
      421 gctgtcgttc gtgtggccgg accagctctc acggatccgc ctgctgcgcg gcgcgatcga
      481 agtagcccgg aaggtcccgg cggcggtcga cgaggagccg gcgtcgtcgt ggctgcggag
      541 cctcgcgcgg ccgcgggacg gcctcgcgac cgtcgtgttc cactcgatag tccaccagta
      601 cctcggcgac gaagaagctg tcgcggtcac ggacgcgctc gaggcggcgg ccggagccgc
      661 gacggcgacc gcgccgttcg cctatctcac gatggagccc ggcgacgacg agaaggtcgt
      721 cgaggtgcgg ctcaccacgt ggcccggcgc tgccgaggag ctactcgcgc acagcggcta
      781 ccacggcacc gccgtgaggt ggctcggcta gtaggccttc atccggccct cgtcgggcgc
      841 acggtctacg cagtcctcga tgcacgtctc gccgctcgtg tccttgctct tcgctatcag
      901 gtcgaggttg ttcgaatcga gctgcgggag gtcgagcgtc atcgtcacgc ggaccgtgcg
      961 tcggttcggc ttcttcgcca cgaggggctc gtactggctg cccccgtcgg tcgagcggag
     1021 gttgaacacg agcgtccctc cgcggcacac gaacgacccg acgaggtcga agtcgttgtt
     1081 gcccgagccg tcgaagaacc acttcagcga cgtcttcgcg gccggccgca gcgccccgca
     1141 cttccacccg gagtgcgtct tgatcgtgag cgtcgcgacg tcctcgggat cctcgacgaa
     1201 cttgatcagc gagatgtcca gcttcagccc ggtgtcggcc gggtcctgct gttcgaccgc
     1261 caacgcgggc gccgcccaga ggcacgacag catcacggcg acggcagaaa cacggagcaa
     1321 acggttgcgc atcggaggac ctccctgtcc ggcgggccag cgagggtcat aatgccgcaa
     1381 gtgaccggcg gatcacgtcc ctacgcaccc ctcggcgtcg cgatcggcgt cttcgtcgtg
     1441 ctggtcgtgg tgctgctgct cgtcgggggc ctcggcgcgc agagcccgcc gaacgagcac
     1501 cgcgagccgg ccaccgacgg gcgcggcgac gtctcgatcg gcgtgagctg gagccccccg
     1561 ttcgagaagt tcgacacctg cgtgatctcg ccgttcgagc cgggggccga g
//
LOCUS       DASQDO010000194         5992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_81397, whole genome shotgun sequence.
ACCESSION   DASQDO010000194 DASQDO010000000
VERSION     DASQDO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81397"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VEV43_05600"
     CDS             <1..798
                     /locus_tag="VEV43_05600"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYP23029.1"
                     /translation="RPVFVIGAPRSGTSLLYAVLRAAPAFAHWPGEAHEVWEADYHPA
                     LRGWSSNALTAADVDPKAAQRIKRAFFLVAGSRRRLIDKTPRNALRVSFVDALFPDAR
                     YVHLVRDGRENVNSLINAWRTPRYRTYRLPHAIEGVDPEWWKFVLYPDWQRDASGPLE
                     VVCARQWASSNERALRDLQAVGDRVVRVRYEDLVDAPEAEVARVLEFLEVPFGESLRA
                     KAAAVRTTPVNVVTPPERGKWRKENPREIEAIEPLIAPAMEALGYEI"
     gene            788..1192
                     /locus_tag="VEV43_05605"
     CDS             788..1192
                     /locus_tag="VEV43_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYP23030.1"
                     /translation="MRSEVTTHDTSSMRILDLTDELHRFARSAGADGLVHVFVPHATA
                     GLAIMETGSGSEGDLLDALERLLPRDDRYRHSHGSRGHGGDHVVPAFVSPSVSIPVVD
                     GAPALGTWQSLVLVDTNVDNPQRKVRWSFLPA"
     gene            1189..1707
                     /locus_tag="VEV43_05610"
     CDS             1189..1707
                     /locus_tag="VEV43_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYP23031.1"
                     /translation="MRDEFDGGRASARYDAAEKEALADTLDFHRATIKWKLEGLSLED
                     AARPMTPSETKLLGIVKHLAYVEVWWFQDNFLGRDCKYPWDETGDMEDDFRIFPGETI
                     ESIVALYDEKCEESRRIVAAASLDDRAARLRRETGYPNLRWIMLHMIEEVARHNGQAD
                     ILRVLLDGTTGA"
     gene            complement(1773..2981)
                     /locus_tag="VEV43_05615"
     CDS             complement(1773..2981)
                     /locus_tag="VEV43_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23032.1"
                     /translation="MALPNVTITRDDLTSLLQPQEGLTVSLYHPTVQAAVEPEESSLH
                     LKNLLARAKNELVERGVGRQEIDDLLAPAYDLLGDRTFWQHQLQGLAVFLSPGGLRHF
                     RLPFEVGEMVSIGDSVHLKPLLPVLNLQGHFFVLALAQDRVQMYRATQHSIEEIDLTR
                     WSIPTSLDEALRYDDLQKPESQHPNVTGPGRGQANDAVVGDAPNQRTHAFHGHGEDGD
                     DPKERIRRFFNVLDDGLSKPLAAETAPLVLAGVEYLRPIFKSATSYDHVLDEGVDGNP
                     EGFRPDEIHAKAWNVVQPYFRERIRDSKDRYGAGVVRGTASDDLPDILAAAYEGRVGV
                     LFALRGVEAWGIFDLAGRRADVHAERGPGDVDLTDLACRQTLATGGEVFVVEEADMPS
                     ATPVAAVYRY"
     gene            complement(3040..3942)
                     /locus_tag="VEV43_05620"
     CDS             complement(3040..3942)
                     /locus_tag="VEV43_05620"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYP23033.1"
                     /translation="MTAPPFFIVGSARSGTTLLRLMLNAHPEVAVPPESRFVAELWTG
                     AERVDVRELLAKLAAHPRFQAWDLPIEMVEHELDGASEAPYPVAIEAAYRAYARVNGK
                     ARWGDKTPRYVESIPLLAKLWPESRFVHLVRDGRNVALSYADVPVGPKNVVKAAQLWA
                     TRVRMGIDAGRTLAHGRYIEIRYEDLVDDAAGEAKDLCDFLDLDFDTGMLDYTERARG
                     AVLPRAKKYNPHVAERPISKVRSWEESMPPAHVEVFEAVAGDVLSELGYERRHPKPGP
                     RARALAAAGKAGLPVTALKGTKRA"
     gene            complement(3944..5173)
                     /locus_tag="VEV43_05625"
     CDS             complement(3944..5173)
                     /locus_tag="VEV43_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYP23034.1"
                     /translation="MTAPNVLLLAIDSLRADAVFGDRIPTPRIDAFAAKGAAFTQCVS
                     TTTSTTPSFSSILTGCYPPKHGVRGLQGYRLSETVTTMAEAFSGAGYKTHAEVTGPLL
                     PQTGVLRGFEDARHRQGYKQPFFGWRDEVVERMHSYVDPWFMLLHIWEVHRPYRAPPD
                     FTKRWDKAGYEAAVAATDEWLAPVYEAAGENTIVVITGDHGEDYPDTPLEQKMIRAAR
                     KARSKLALGKWFPYADKKIQALAVGHGFALFEHLVRVPLIVSGPGVPAAQVGEQVRHV
                     DLFPTLADVCGVAIPDGIDGRSLRPLIEGGSLTPEPAYMEAVGVKLEGNRISGARTPD
                     FKLLRRGDSKPALYKLNGGDGANEKRNVIGRYPEVGRSLEDFIDRVLKTEVVAESGMT
                     EDEEATVEQHLRDLGYL"
     gene            5257..>5992
                     /locus_tag="VEV43_05630"
     CDS             5257..>5992
                     /locus_tag="VEV43_05630"
                     /inference="COORDINATES: protein motif:HMM:NF021997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYP23035.1"
                     /translation="MSHRAALSYRAANPDPEASEIDRRTFLKAAGGSAAAALLAACEN
                     ETTPSPTPPPPDRVVEADFAGTHRGWGPDWVNVRYEGPWSVEEGRGVIEVEPAVEKAL
                     QEGKEVAEYMARPVISRLVDADEVEVAATVELDGPVEAGVVARVSYEEGYALLVRDGE
                     ARLYRYDVVDRKLLAKESLDTAGPVELKLVVRGADITATAGSVRLTATDDDPLERGFA
                     GVVVNPASTSEGGRALFDEVAIVAAPG"
BASE COUNT          857 a         2056 c         2184 g          895 t
ORIGIN      
        1 cgcccggtgt tcgtcatcgg agcgccgcgg tcggggacgt cgctgctcta cgcggtgctg
       61 cgggcggccc ccgccttcgc tcactggccc ggcgaggccc acgaggtctg ggaggccgac
      121 taccacccgg cgctgcgggg atggagctcg aacgccctca cggccgcgga cgtcgacccg
      181 aaggccgcgc agaggatcaa gcgggcgttc ttcctggtcg cggggtcgcg gcggaggctg
      241 atcgacaaga caccgcgcaa cgcgctgcgg gtctcgttcg tcgacgcgct gttccccgac
      301 gcgcgctacg tccacctcgt gcgcgacggc cgcgagaacg tgaactcgct gatcaacgcg
      361 tggcgcacgc cgcgctaccg cacgtaccgt ctgccgcacg cgatcgaggg cgtcgacccc
      421 gaatggtgga agttcgtgct gtacccggac tggcagcggg acgcttcggg gccgctcgag
      481 gtcgtgtgcg cccgccagtg ggcgtcgtcc aacgagcgcg ctctgcgcga cctccaggcg
      541 gtgggggacc gggtcgtgcg ggttcgctac gaggacctcg tcgacgcgcc ggaggccgag
      601 gtcgcgcgcg tgctggagtt cctcgaggtc ccgttcggcg aaagcttgcg ggccaaggcc
      661 gcggccgtgc gtacgacgcc ggtcaacgtc gtgacgccgc ccgagcgggg gaagtggcgt
      721 aaggagaacc cgcgcgagat cgaggcgatc gagccgttga tcgcacctgc gatggaggcg
      781 ctcggctatg agatctgagg taacgaccca cgacacctcg tcgatgcgga tcctggacct
      841 gacggacgag ctccacaggt tcgcgcgctc ggctggcgcg gacgggctcg tgcacgtctt
      901 cgtccctcac gccaccgcgg ggctggcgat catggagacc gggtcgggct ccgagggcga
      961 cctgctcgac gcgctcgaac ggctgttgcc gcgcgacgac cgctaccgtc acagccacgg
     1021 ttcgcgcggc cacggcggcg accacgtcgt gccggcgttc gtgtcgccgt cggtgtcgat
     1081 cccggtcgtc gacggagcac cggcgctcgg aacgtggcag agtctcgtcc tcgtggacac
     1141 caacgtcgac aacccgcagc gcaaggtgcg ctggtcgttc ctgccggcat gagggacgag
     1201 ttcgacgggg gccgcgcctc ggctcgctac gacgcggccg agaaggaggc cctcgccgac
     1261 acgctcgact tccaccgggc gacgatcaag tggaagctcg agggcctgtc gctggaggac
     1321 gccgcccgtc cgatgacgcc gagcgagacg aagctgctcg ggatcgtgaa gcacctcgcc
     1381 tacgtcgagg tgtggtggtt ccaggacaac ttcctcggcc gcgactgcaa gtacccgtgg
     1441 gacgagaccg gcgacatgga ggacgacttc cggatcttcc ccggcgagac gatcgagtcg
     1501 atcgtcgccc tctacgacga gaagtgcgag gagtcgcgcc ggatcgtcgc cgccgcgtcg
     1561 ctggacgacc gggcggcgcg gctccgccgg gagacgggct acccgaacct gcgctggatc
     1621 atgctgcaca tgatcgagga ggtggcccgg cacaacggcc aggccgacat cctgcgggtg
     1681 ctgctggacg gtacgacggg ggcctgaggc ccccgtccgc acgagtgagg aaaaacggac
     1741 gctatgcgtc cagttttcct cagaggtctt ggctagtagc ggtagaccgc ggctaccggg
     1801 gtcgccgagg gcatgtcggc ctcctcgacc acgaagacct cgccccccgt cgccagcgtc
     1861 tggcggcacg cgaggtcggt caggtccacg tcgcccggcc cccgctccgc gtgcacgtcc
     1921 gctctccggc ccgccaggtc gaagatcccc caggcctcga ccccccgcag cgcgaacagg
     1981 acgcctacgc ggccctcgta tgcggcggcc aggatgtccg gcaggtcgtc cgacgccgtc
     2041 ccccggacga cgcctgcgcc gtagcggtcc ttcgagtcgc ggatgcgctc gcggaagtac
     2101 ggctgcacga cgttccaggc cttcgcgtgg atctcgtcgg gccggaagcc ctcggggttg
     2161 ccgtcgacgc cctcgtcgag gacgtggtcg tagctcgtcg cgctcttgaa gatcggccgc
     2221 aggtactcga cgccagcgag gacgagcgga gcggtctcgg ccgcgagggg cttcgacagg
     2281 ccgtcgtcga ggacgttgaa gaaccggcgg atcctctcct tcgggtcgtc gccgtcctcg
     2341 ccgtgaccgt ggaacgcgtg cgtgcgctgg ttcggcgcgt cgcccacgac cgcgtcgttc
     2401 gcctgaccgc ggcccggccc ggtcacgttc gggtgctgcg actcgggctt ctgcaggtcg
     2461 tcgtaacgca gcgcctcgtc gaggctcgtc gggatcgacc acctcgtcag gtcgatctcc
     2521 tcgatcgaat gctgcgtcgc ccggtacatc tggaccctgt cctgcgcgag cgcgagcacg
     2581 aagaagtggc cctgcaggtt gagcaccggc agcagcggct tgaggtggac ggagtcgccg
     2641 atcgacacca tctcgccgac ctcgaacggg agccggaagt ggcgcagccc gccgggggag
     2701 aggaacaccg cgaggccctg cagctggtgc tgccagaacg tccggtcgcc gagcaggtcg
     2761 tacgccggcg cgaggaggtc gtcgatctcc tgacgaccga cgccgcgctc gacgagctcg
     2821 ttcttcgccc gcgcgagcag gttcttgaga tggagcgagc tctcttcggg ctcgaccgcc
     2881 gcctggaccg tcgggtggta cagcgacacc gtgaggcctt cctgcggctg cagcagcgag
     2941 gtgaggtcgt cgcgcgtgat cgtcacgttc ggcagggcca tcgttccccc ttggcttcgt
     3001 gagttcggtg gcccggtacc taccccggag cggggggcgc tatgcccgct tcgtcccctt
     3061 cagagcggtg acggggaggc cggccttgcc cgccgccgcc agggcgcgcg cacgggggcc
     3121 gggcttggga tggcgccgtt cgtatccgag ctccgacagg acgtcgccgg ccacggcctc
     3181 gaacacctcg acgtgcgcgg gcggcatcga ctcctcccac gaccgcacct tcgatatcgg
     3241 gcgctcggcg acgtgcgggt tgtacttctt cgcccggggt agcacggcgc cgcgcgccct
     3301 ctcggtgtag tcgagcatgc cggtgtcgaa gtcgaggtcg aggaagtcgc agaggtcctt
     3361 cgcctcgccg gccgcgtcgt ccacgaggtc ctcgtagcgg atctcgatgt agcgcccgtg
     3421 cgcgagcgtg cgccccgcgt cgatccccat gcgcacgcgg gtcgcccaca gctgcgcggc
     3481 cttgacgacg ttcttcggac cgaccgggac gtccgcataa gagagcgcga cgttgcgccc
     3541 gtcccgcacg aggtggacga agcggctctc cggccacagc ttggcgagca gcgggatgct
     3601 ctcgacgtag cgaggggtct tgtcgcccca gcgcgccttg ccgttcacgc gcgcgtaggc
     3661 acggtacgcc gcctcgatag cgacggggta gggggcctcg gacgcgccgt cgagctcgtg
     3721 ctcgaccatc tcgatcggga ggtcccacgc ctggaagcgc gggtgcgcgg cgagcttcgc
     3781 gagcagctcg cgcacgtcga cgcgctcggc ccccgtccac agctcggcca cgaagcgcga
     3841 ctccggcggc accgcgacct cgggatgcgc gttgagcatc agccggagca gcgtcgtccc
     3901 cgagcgcgcc gacccgacga tgaagaacgg aggtgcggtc acgtcagaga tagccgaggt
     3961 ccctcaggtg ctgctcgacc gtcgcctcct cgtcctcggt catgccggac tcggctacga
     4021 cctcggtctt caggacgcga tcgatgaagt cctccagcga ccgccccacc tccgggtagc
     4081 ggccgatcac gttgcgcttc tcgttcgccc cgtcgccgcc gttgagcttg tagagcgcgg
     4141 gcttcgagtc gccccggcgc agcagcttga agtcgggcgt gcgcgcgccg gagatgcggt
     4201 tgccctcgag cttgacgccg accgcctcca tgtacgcggg ctccggtgtc agcgagccgc
     4261 cctcgatcag tggccgcagg ctgcgtccgt cgatcccgtc cgggatcgcg actccgcaga
     4321 cgtcggcgag cgtcgggaac aggtcgacgt ggcgcacctg ctcgcccacc tgagccgcgg
     4381 ggacgccggg gcccgagacg atcagcggga cgcgcacgag gtgctcgaac agagcgaagc
     4441 cgtggccgac cgcgagggcc tggatcttct tgtcggcgta cgggaaccac ttgccgagcg
     4501 cgagcttgga gcgcgccttg cgggccgcgc ggatcatctt ctgctcgagc ggggtgtccg
     4561 ggtagtcctc gccgtggtcg ccggtgatga ccacgatcgt gttctcgccg gcggcctcgt
     4621 agaccggcgc gagccactcg tccgtcgcgg cgacggccgc ctcgtacccc gccttgtccc
     4681 accgcttggt gaagtcgggc ggagcgcgat acggacgatg gacctcccag atgtgcagca
     4741 gcatgaacca ggggtcgacg tacgagtgca tgcgctcgac gacctcgtcg cgccagccga
     4801 agaacggctg cttgtagccc tggcggtgac gggcgtcctc gaagccgcgc agcacgccgg
     4861 tctgcgggag cagcggcccg gtcacctcgg cgtgggtctt gtagccggcg cccgagaacg
     4921 cctcggccat cgtcgtgacg gtctcggaga ggcggtagcc ctggaggccg cgcacgccgt
     4981 gcttgggcgg gtagcagccc gtgaggatcg acgagaagga cggcgtcgtc gacgtggtgg
     5041 tcgagacgca ctgtgtgaac gcggccccct tggcagcgaa ggcgtcgatg cgaggggtcg
     5101 ggatccggtc gccgaacacg gcgtcggctc ggagcgagtc gatcgccagc agaaggacgt
     5161 tcggcgcggt caagcgggac ctccgtgact tcgttggaac gacgatgttg cgtggaccgc
     5221 tcgccgaggc gaccggtgcc agaggcgccg gggcgtgtgt cccaccgggc ggccctatcc
     5281 taccgggcgg ccaaccccga cccggaggcg agcgagatcg acagaaggac gttcctgaag
     5341 gccgcaggag gctccgcggc cgcggctctc ctggccgcgt gcgagaacga gacgacgccc
     5401 tcgccgacgc ccccgccccc cgaccgagtc gtggaggcgg acttcgcagg cacccatcgc
     5461 ggatgggggc cggattgggt caacgtgcgc tacgaggggc cgtggtcggt cgaagagggc
     5521 cgcggcgtca tcgaggtcga gcccgccgtc gagaaggcgc tgcaggaggg caaggaggtc
     5581 gccgagtaca tggcgcggcc cgtgatctcg cgcctggtcg acgcggacga ggtcgaggtc
     5641 gcggcgacgg tcgagctcga cggcccggtc gaagccggcg tcgtcgcgcg cgtctcttac
     5701 gaggagggct acgccctgct cgtgcgcgac ggagaggccc gcctgtaccg ctacgacgtg
     5761 gtcgaccgga agctcctggc gaaggagagc ttggacactg cgggcccggt ggagctgaaa
     5821 ctcgtcgtgc ggggagccga catcaccgcg acggccgggt ccgtcaggtt gacggcgacg
     5881 gacgacgacc ctctcgagcg cggcttcgcc ggcgtcgtcg tgaacccggc ctctacgtcg
     5941 gaggggggcc gcgcgctctt cgacgaggtc gcgatcgtgg cggccccggg cg
//
LOCUS       DASQDO010000195         4108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_876795, whole genome shotgun sequence.
ACCESSION   DASQDO010000195 DASQDO010000000
VERSION     DASQDO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876795"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VEV43_05635"
     CDS             complement(<1..737)
                     /locus_tag="VEV43_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005257808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP23036.1"
                     /translation="MNVSGKVALVTGGASGLGGATVKALHDAGASVVILDLPSSEGEV
                     RAKELGDRARFAPADVTNESEVGAAVELAANDFGGLHVLVNCAGVGWPGRVLTREGKA
                     TDLERFEAVIRINLIGTFNVIRLAAQQMASQEPDGEERGVVVNTASIAAFDGQIGQAA
                     YAASKGGVAGLTLPIARDLASKLIRCATIAPGIFDTPMLAGLPEEARQKLAENVPHPH
                     RLGNPAEYAALVRHIVENPMINGEVLRL"
     gene            complement(734..2680)
                     /locus_tag="VEV43_05640"
     CDS             complement(734..2680)
                     /locus_tag="VEV43_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP23037.1"
                     /translation="MPRRGHGLVPPDAPEKPARPGTFKRIARTFRPYRPQVILVAILI
                     LVTSGLGLVNPLMIKVVFNEVFIFEGTDSEKLRTLLIVVGIMVVTPIVSGALGLWQTY
                     TNNLVGQHVMQDLRNSLYAHLQHMPLRFFTSTRTGEIQSRLSNDVGGVQGVLTDTASS
                     ILANISTVLSTVVAMFLLSWELTVLSLAILPLFMILSKKVGQVRREVSGRTQESLADL
                     TAHMQETLSVSGILLSKAFGRQAYEIDRFRKENQRLSRLEIKRTMVGRGFFALVQIFF
                     SITPAFVYLVAGIMFLNGSVGPEIVGTIVAFTTLQARLFFPMGQLLNVNVEIQSSLAL
                     FDRIFEYLDLEREIVDRDGAVAVAPQGVRGEVAFQDVWFKYDKTDHDVTHDRDWTIAG
                     VDFTMQPGELVALVGPSGAGKTTLTYLLPRLYDVDRGAVRIDGRDVRDVKLESLGEII
                     GMVTQETYLFNSSVRDNLRYGNPDATDGEIEAAARAAHIWERIAELPEGFDTVVGERG
                     YKMSGGEKQRLAIARVILKDPRILVLDEATSALDTHSERLVQEALSPLMQGRTTLAIA
                     HRLSTILAADTILYVDRGRIVERGTHSELLEKGGAYAALYHEQFSDGRVETRCEDGFV
                     LTSGEVIQEDGSPGRAAQIQEEAV"
     gene            complement(2682..>4108)
                     /locus_tag="VEV43_05645"
     CDS             complement(2682..>4108)
                     /locus_tag="VEV43_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYP23038.1"
                     /translation="ARRAGALLLERFAAAPTGVRTKSTSTDLVSDADTASERLIFETV
                     SAARPGDGWIAEEGTSAASTTGITWVADPLDATVNYLFRIPVWCVSIAAEDGDGPLAG
                     VVHDPNRNETFTATRGGGAFRNGEPIRVSDRADLATALIATGFEYDRDLRAFQARVVA
                     EVLPQVRDVRRMGSAALDLASVACGRVDGYYESSTERWDRAAGALLVKEAGGVVTELT
                     NPSGRTPGVVAAGPGIHAALEALVGRARPEFPAACSVRFRRMDIVNPAVERYLRELAA
                     GDDEPVLLEMEKLAEDEGFPIVGRLCGRYLEVLARSISARRIFEMGSGYGYSGYWFSR
                     ATGPEGEIHLTDGDPENERKALDFLKRAGLDAPIRYHVGDALEELGKVDGEFDVVYCD
                     IDKLDYLKAWEAGKERVRVGGYYVCDNMLWSGRVTGEAGANDDRPHWTEAIMATNRAI
                     AADPGFRETINPTRDGVVAALRIA"
BASE COUNT          613 a         1464 c         1391 g          640 t
ORIGIN      
        1 aggcggagca cttccccgtt gatcatcggg ttctccacta tgtgtcgcac cagggccgcg
       61 tactccgccg ggttccccag gcggtgcggg tgcggcacgt tctcggccag cttctgcctc
      121 gcctcttccg gcaggcccgc cagcatcggc gtgtcgaaga tccccggcgc gatcgtggcg
      181 cagcggatca gcttcgaggc caggtctcgg gcgatcggga gcgtcaggcc cgccacgccg
      241 cccttcgacg ccgcgtaggc cgcttggccg atctggccgt cgaacgcggc gatgctcgcg
      301 gtgttcacga cgacgccgcg ctcctcgccg tcgggctcct gcgacgccat ctgctgggcc
      361 gccagccgga tcacgttgaa cgtcccgatc aggttgatgc ggatcacggc ctcgaagcgt
      421 tcgaggtctg tcgccttgcc ctcgcgggtg agcactcgcc ccggccaccc gacgccggca
      481 cagttcacga gcacgtgcag gccgccgaag tcgttcgccg cgagctcgac cgcggccccc
      541 acctcggact cgttcgtcac gtcggcggga gcgaagcgcg cgcggtcgcc gagctccttc
      601 gcacgtacct cgccctcgga cgagggaagg tcgaggatca cgacggacgc gccggcgtcg
      661 tgcagcgcct tcacggtcgc gccgccgagc cccgacgctc ctcccgttac gagcgcgacc
      721 ttccccgaga cgttcacacg gcctcctctt ggatctgggc ggctcgccca ggcgacccgt
      781 cctcttgaat gacctctccg ctggtcagga cgaacccatc ctcgcatctg gtctcgacgc
      841 gcccgtcgga gaactgctcg tggtacagcg ccgcgtacgc gccgcccttc tccagaagct
      901 ccgagtgcgt cccgcgctcg acgatccggc cccggtcgac gtagaggatc gtgtcggccg
      961 cgaggatcgt cgacagacgg tgcgcgatcg ccagcgtcgt ccgtccctgc atcaacggcg
     1021 acagggcctc ctgcacgagc cgttccgagt gggtgtcgag cgcgctcgtc gcctcgtcga
     1081 gcacgaggat gcgcgggtcc ttgaggatca cgcgggcgat cgcgagcctc tgcttctcgc
     1141 cgcccgacat cttgtagccg cgttcgccga cgacggtgtc gaagccctcg gggagctcgg
     1201 cgatgcgctc ccagatgtgg gccgcgcgcg cggcggcctc gatctcgccg tcggtagcgt
     1261 cggggttccc gtagcgcagg ttgtcgcgca cgctcgagtt gaacaggtac gtctcctgcg
     1321 tgaccatgcc gatgatctcg ccgagcgact cgagcttcac gtcgcggacg tccctgccgt
     1381 cgatccggac cgcgccgcgg tcgacgtcgt agagccgggg gagcaggtag gtcagcgtgg
     1441 tcttgccggc ccccgagggc ccgacgagcg cgaccagctc gcccggctgc atcgtgaagt
     1501 cgaccccggc gatcgtccag tcgcggtcgt gggtgacgtc gtggtcggtc ttgtcgtact
     1561 tgaaccagac gtcttggaac gcgacctcgc cgcgcacgcc ctggggcgcg acggctaccg
     1621 cgccgtcgcg gtcgacgatc tcgcgctcga ggtcgaggta ctcgaagatc cggtcgaaca
     1681 gcgcgagcga cgactggatc tcgacgttca cgttgagaag ctggcccatc gggaagaaca
     1741 gccgcgcctg caaggtcgtg aacgcgacga tcgtcccgac gatctcgggc cccaccgaac
     1801 cgttgaggaa catgatcccg gccaccaggt agacgaacgc cggcgtgatc gagaagaaga
     1861 tctgcaccag ggcgaagaag ccgcggccca ccatcgtccg cttgatctcg agccgcgaca
     1921 gccgctggtt ctccttgcgg aaccggtcga tctcgtaggc ctggcggccg aacgccttag
     1981 acagcaggat cccagagacc gacagcgtct cctgcatgtg cgcggtgagg tcggcgagcg
     2041 actcctgcgt ccggcccgac acctcacgcc gcacctggcc gaccttcttc gacaggatca
     2101 tgaacagcgg gaggatcgcg agcgacagga ccgtgagctc ccacgagaga aggaacatcg
     2161 cgacgaccgt ggagagcacc gtcgagatgt tcgccaggat gctcgacgcg gtgtcggtga
     2221 gcacgccctg gacgccgccg acgtcgttgg acaggcgcga ctggatctcg cccgtgcgcg
     2281 tcgacgtgaa gaagcgcagc ggcatgtgct ggaggtgcgc gtagagcgag ttgcgcaggt
     2341 cctgcatcac gtgctggccg acgaggttgt tcgtgtacgt ctgccacagc ccgagcgcgc
     2401 cggagacgat cggcgtcacg accatgatcc cgacgacgat caacagcgtg cgcagcttct
     2461 cggagtccgt cccctcgaag atgaagacct cgttgaagac gaccttgatc atcaacgggt
     2521 tgacgaggcc gagccccgac gtcacgagga tcaggatcgc gacgaggatc acctgggggc
     2581 gataggggcg gaacgtcctc gctattcgct tgaaggtccc gggccgggcc ggcttctcgg
     2641 gtgcatccgg cgggacgagc ccgtgaccgc gccgcggcaa ctcaggcgat cctgagcgcg
     2701 gccaccacgc cgtcgcgcgt ggggttgatc gtctcgcgga agcccgggtc ggcggcgatc
     2761 gcgcggttcg tggccatgat cgcctcggtc cagtgcgggc ggtcgtcgtt ggcgccggcc
     2821 tcgccggtga cccgccccga ccacagcatg ttgtcgcaga cgtagtagcc gccgacgcgc
     2881 acgcgctcct tcccggcctc ccacgccttc aggtagtcga gcttgtcgat gtcgcagtag
     2941 acgacgtcga actcgccgtc gaccttcccc agctcttcga gcgcgtcgcc gacgtggtag
     3001 cggatcggcg cgtcgagccc ggccctcttc aggaagtcca gcgccttgcg ctcgttctcg
     3061 gggtcgccgt ccgtgaggtg gatctcacct tccggcccgg tcgcgcgcga gaaccagtag
     3121 cccgagtagc cgtagcccga gcccatctcg aagatgcgcc gggcggagat cgatcgcgcg
     3181 agcacctcca ggtagcggcc gcagagccgg ccgacgatcg ggaagccctc gtcctcggcg
     3241 agcttctcca tctcgagcag aacgggctcg tcgtcgcctg cggcaagctc gcgcaggtag
     3301 cgctcgactg cggggttcac gatgtccata cgcctgaacc taacggagca ggccgctgga
     3361 aattccgggc gcgcccgccc gaccagggcc tccagcgccg cgtggatgcc cggccccgcg
     3421 gcgacgacgc cgggcgtccg gcccgagggg ttcgtcagct cggtgacgac gccgccggcc
     3481 tccttcacga gcagggcgcc cgcggcacga tcccagcgct cggtgctcga ctcgtagtag
     3541 ccgtcgacgc ggccgcaggc gacgctcgcc agatccagcg cggccgagcc catccggcgg
     3601 acgtcccgca cctgcggaag gacctccgcc accacccggg cttggaacgc gcgcaggtcg
     3661 cggtcgtact cgaacccggt cgcgatcagg gccgtggcga ggtcggctcg gtcgctcaca
     3721 cggatcggct cgccgttccg gaacgcgccc ccgccgcgcg tcgcagtgaa cgtctcgttc
     3781 cggttcgggt cgtggacgac gccggcgagc gggccgtcgc cgtcctcggc cgcgatgctc
     3841 acgcaccaca ccgggatacg gaagagatag ttgacggtcg cgtccagcgg gtccgcgacc
     3901 cacgtgatgc cggtcgtcga cgcggcactc gtcccctcct cagcgatcca gccgtcaccc
     3961 ggccgggccg cggagaccgt ctcgaagatc agccgttccg acgcggtgtc cgcgtcgctc
     4021 acgaggtcgg tcgacgtcga cttcgtccgc acgcccgtcg gagcggcggc gaacctctcc
     4081 agaaggaggg cccccgctcg gcgcgcgg
//
LOCUS       DASQDO010000196         9372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_81500, whole genome shotgun sequence.
ACCESSION   DASQDO010000196 DASQDO010000000
VERSION     DASQDO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81500"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEV43_05650"
     CDS             <1..424
                     /locus_tag="VEV43_05650"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYP23039.1"
                     /translation="WIFVARAVRAVLIELRPAKAAAPARGAPVPSQPPPRKSKGKPKL
                     PGKAVVIEGTTLKGKSFPLTGELTIGRSDQCKVHVEGDTYVSTMHARIFQRDGSYMVE
                     DLGSTNGTYLNRRRITAPAELQRGDRVKVGKTVLEMRK"
     gene            421..1620
                     /locus_tag="VEV43_05655"
     CDS             421..1620
                     /locus_tag="VEV43_05655"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HYP23040.1"
                     /translation="MNVAVGVQTDVGRVRKRNEDSYLLEAPIYGVADGMGGHIAGDVA
                     SATAVASITEGIGSGRPEDATALAELVSRANGAVWEKARSDPSLRGMGTTCTVMMIDD
                     GVAHVAHVGDSRAYLLRDGSFRQITEDHTLVERMVREGKLSAEEAANHPQRNIITRTL
                     GVDANVQVDAFDERLADGDRILITSDGLTSMVEGREIATVLDQEEDPQAAADRLVEMA
                     NDAGGEDNITVVVLHVGKPGPNDGSPAPPPQAPARHDTRSGAEPEVAAGPGGRRWVGP
                     LLKTLTVLAVLAVAGVFTVRWVLSNSWYVRLSDEGVVTIYEGIPEEIAGMSFSDVREE
                     SDVAEADIPESFRDELEDGKKFESLEDAQGYVAAIEHRIEEIEESTEQEPDRDRNRDR
                     DGKKGSS"
     gene            1617..3014
                     /locus_tag="VEV43_05660"
     CDS             1617..3014
                     /locus_tag="VEV43_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HYP23041.1"
                     /translation="MSAQPALRRNRELSLLVLALIVGAGALTLVAVTRNSNKPAVAVP
                     LIVIVALAYAGAHLITRRFAKQADSLVLPLVAGLNAVGLAAIYRLSPKGFGATQVTWT
                     ALGLACFAGTLIVVRDYRALSRYKYILGFAGIALLLLPMTPVGAVVHGARLWVRFGGF
                     QFQPAELSKICLVAFFAAYLAERKELLAVATKRIAGFHVPDFKHFGPLLVMWGVSLAV
                     MFYERDLGSSLLFFSVFLLMLYVATSRVVYTAFGALLFVGGAIVGYQVFTHVQSRVQV
                     WLDVFDPELIQGASYQLAQSLFALATGGLFGTGLGQGRPDLIPVAETDFIFAVVGEEL
                     GLMGTVAVLLTFLLLVGRGLRVALHARDDFGQLLAAGLTFILGLQTIIILGGVTRLMP
                     LTGITLPFMSYGGSSILSNFILVALLLRISDQSEGAPPPAHTAEILIGGRLGGTSEQS
                     GARLGGTPEQSGGRP"
     gene            3011..4483
                     /locus_tag="VEV43_05665"
     CDS             3011..4483
                     /locus_tag="VEV43_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYP23042.1"
                     /translation="MMSAQIRKVGIGLLLAFTALFLQLNYIQIWAAEDIASNPNNIRA
                     LIAEYSVKRGDIETHDGKLVATSRESKGRYKYERVYPEGDLYAHIVGYKSIVYGETRL
                     EAAFDEQLQGKTAVQSMQDLEDTLSGGEEQGDDLRLTIHHQLQEKARDLLDGRDGAIV
                     ALNPQTGEVRAMWSNPSYDPNPLASHDTKEVRRYRLTLDPNSNTSPLISRATSRSFAP
                     GSTFKVVTTAAALESGQYRANSTFPDPVALDLPQTDDTLQNFTRTACTSAGRIDLFTA
                     LEVSCDTTYGILGMEIYDQLRETSEAMGFNEAIPFDVGTEASTFPDVGDDNIPFRAFA
                     GIGQGDVSATPLQMALVAATVANGGEVPRPRLVRQVIDPSGGIVEPFTPETIGRAMSQ
                     ETAARVNEMMQAVVLSGTGTSARIEGVPVAGKTGTAQTVPGESPHAWFIAFAPADDPQ
                     LAVAVIVESSSAGSEATGGAIAAPLAKQILETDRAISAAW"
     gene            4477..5889
                     /locus_tag="VEV43_05670"
     CDS             4477..5889
                     /locus_tag="VEV43_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYP23043.1"
                     /translation="MVAGRVLGDRYRIEERIAAGGMGTVYAATDDRLHRRVAVKLLKE
                     GLSGDAKFVARFEREARAAAALSHPNVAGVFDYGEDGETPFIVMELAQGRDLARLLRD
                     EGPLEPDRARGLGAQICTALAHAHAAGLVHRDVKPANVIVDESDRVKVTDFGIARAAG
                     EATLTATGSVLGSAQYMAPEQASGAPVTAAADVYAAGIVLYEMLTNAVPFTGDSPVSV
                     AMRHVSDEVPRPSSVNPQVPESLDAIVARATAKDPADRFPSAAAMGAALEGDEVTAPV
                     AAGEATQVITSSGAAAETSVWPIPGNRYDPRRLGTRVLAVAAILAIAAVVTWGWLLSR
                     SGDAVRDRRRAPAAARPTQQPAQAGATEPTEPASDGYELPSFTGMPFKEAEKVLVEQG
                     IVVVPLLQPSSEERDEVVGSEPVAGTVVAAGDTVTLLISDGAGGDEGEDSDEEGPGNS
                     EDAPGHDKDDKDKEKDKDEE"
     gene            5886..7796
                     /gene="pknB"
                     /locus_tag="VEV43_05675"
     CDS             5886..7796
                     /gene="pknB"
                     /locus_tag="VEV43_05675"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HYP23044.1"
                     /translation="MSATNRTYGGRYAVLEPVGSGGMAEVYRARDELLGRDVAVKVLS
                     ARLSSDKSFVERFRREAQSAANLNHPNVVSLYDYGADDGAYYIVMEYIEGKSLGDIVA
                     ESGALLPERAAEIASDVAAALERAHSSGLVHRDIKPTNIMVTNTGQTKVTDFGIARAL
                     GQSTEQTQMTQTGMVIGTAAYLSPEQAQGNPVDARSDVYSLGCVLYEMLTGRPPFAGD
                     APLAIAYKHVREDPVPPSTVNPDVPRELDAVTLKALSKNPGNRYTTAAEMREDLQRFL
                     NGQKVLATPLMAAETAVAAPVSTGTQVLRTTETEYAPPPPEPRRGVWYVLAALLILAL
                     FGLGAWLLANNFLGGGDEVDVPNVVGLTEEDAVDRLERRGFEVDVEERPSDKPEGDVF
                     RQDPDRGEQAEEGSTVTIFVSTGPEAFEVPDLRGEELRDARRELKQADLTVGTITRQP
                     SEDVPPDTVLDQFPRPPAEVRPGDAVDLVVSSGPEAIPAPSVVGQTEDDAIAEIEALG
                     LFADPIQVPDDAPAGEVIGQDPAPGTPMEEGDVVAITVSTGPEGEPMPDVRGDDADTA
                     EAFLENDFGLSVTQEEGACAAPPGAVCDQDPPPDTLVAPGDSAILYVQPGGAALPGDD
                     LFAFLSLLALFA"
     gene            complement(7793..8371)
                     /locus_tag="VEV43_05680"
     CDS             complement(7793..8371)
                     /locus_tag="VEV43_05680"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HYP23045.1"
                     /translation="MKLLLVDNYDSFVYNLAQAFGSLGAEPVVVRNDAALDDLVAVEP
                     DAVVISPGPGTPAEAGVSIAAIEAFAGRVPVLGVCLGHQCIGAAFGGTVNRAPVGPMH
                     GKVSPIEHDGTGVFAGLPSPFEATRYHSLAIDDDGWPDALEVTARATDGVVMGVRHRE
                     LPVEGVQFHPESILTDRGPQLLANFVSAAAPG"
     gene            8394..8852
                     /locus_tag="VEV43_05685"
     CDS             8394..8852
                     /locus_tag="VEV43_05685"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HYP23046.1"
                     /translation="MDDFVEEITRGNVSGVKIVLATVVLALAVYQVLLMAVGYGKLKL
                     PFLQAKAASFAHRSIGDAILLVTLVVAFMCIAYFGYEDGGEHAAGGETGRATLHVIGG
                     TAVVAVLAVKVLAVRWWHALGRFLPVIGMALFGLLALTWATSAGDYFFGS"
     gene            8855..9055
                     /locus_tag="VEV43_05690"
     CDS             8855..9055
                     /locus_tag="VEV43_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23047.1"
                     /translation="MAERKYTYQLIGSLLVGLLIVIVTISIVTGKLGPRLDRREELRE
                     LEDVREERQEQREERREELNDD"
     gene            complement(9165..>9372)
                     /locus_tag="VEV43_05695"
     CDS             complement(9165..>9372)
                     /locus_tag="VEV43_05695"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HYP23048.1"
                     /translation="EMGDPIHFETRWGDFTYRVTDKRIVPADSDAIVIPSKKYEVVLT
                     TCNPKYSAAERLIVYAELEREVAR"
BASE COUNT         1485 a         3257 c         3300 g         1330 t
ORIGIN      
        1 gtggatcttc gtcgcgcgcg cggtgcgcgc agtcctgatc gagctacgcc ccgcgaaggc
       61 cgcggccccc gcacgcgggg cccccgtccc gagccagccg ccgccgcgca agagcaaggg
      121 caagcccaag ttgcccggca aagccgtggt gatcgagggc acgacgttga agggcaagtc
      181 gttcccgctc acgggcgagc tcacgatcgg ccgctccgac cagtgcaagg tccacgtcga
      241 aggcgacacc tacgtgtcga cgatgcacgc gcgcatcttc cagagagacg gctcctatat
      301 ggtcgaggac ctcgggtcta caaacgggac gtacctgaac cggcggcgga tcacggcccc
      361 ggccgagctc caacgggggg atcgcgtcaa agtcggcaag accgtgctgg agatgcgcaa
      421 gtgaacgtcg ccgtcggagt ccagaccgac gtcggacgcg tgcgcaagcg caacgaggac
      481 tcctacctcc tcgaggcgcc gatctacggc gtagccgacg ggatgggcgg ccacatcgca
      541 ggcgacgtcg cgtccgcgac cgcggtcgct tcgatcacgg aagggatcgg atcgggccgg
      601 ccggaggacg ctaccgcgct cgccgagctc gtcagccgcg cgaacggcgc cgtgtgggag
      661 aaggcgcgct cggacccctc cttgcgcggg atgggcacga cgtgcacggt gatgatgatc
      721 gacgacggcg tcgcgcacgt cgcgcacgta ggagattcgc gcgcgtatct gctgcgcgac
      781 gggtcgttcc gtcagatcac cgaggaccac acgctggtcg agcgcatggt gcgcgagggc
      841 aagctctcag ccgaggaagc cgctaaccac ccgcagcgca acatcatcac gcggacgctc
      901 ggcgtcgacg cgaacgtgca ggtcgacgcg ttcgacgagc ggctcgccga cggcgaccgg
      961 atcctcatca cgtcggacgg cctcacctcg atggtcgagg gccgcgagat cgcgaccgtc
     1021 ctcgaccagg aagaagaccc gcaggccgcg gcggaccggc tcgtcgagat ggcgaacgac
     1081 gccggcggcg aggacaacat cacggtcgtc gtcctgcacg tcggcaaacc ggggccaaac
     1141 gacgggagcc cggccccccc tccccaggcc ccggctaggc acgacacccg gtccggggcg
     1201 gaaccagagg tcgccgcggg gcccgggggc cggaggtggg tcgggcctct cctgaagacc
     1261 ctcacggtgc tcgcggtgct cgccgtcgcc ggcgtcttca ccgtccggtg ggtcctgtcg
     1321 aactcctggt acgtccggct ctccgacgaa ggcgtcgtga cgatctacga ggggatcccg
     1381 gaggagatcg ccggcatgag cttctccgac gtccgtgaag agagcgacgt cgccgaggcg
     1441 gacatccccg agtccttccg cgacgagctg gaagacggca agaagttcga gtcgctcgag
     1501 gacgcccagg gatacgtagc cgcgatcgag catcgcatcg aggagatcga ggagtcgacc
     1561 gagcaggagc cggaccgcga ccgcaaccgt gatcgcgacg gcaagaaggg cagctcgtga
     1621 gcgcccagcc agcgctgcgg cgcaaccgcg agctgtctct gctcgtcctc gcgttgatcg
     1681 tgggggccgg ggcgctgacg ctcgtcgccg tcacgcgcaa cagcaacaag cccgcggtcg
     1741 ccgtgcccct gatcgtcatc gtcgcgctcg cctacgcggg agcgcacctg atcacgcggc
     1801 ggttcgcgaa gcaggccgac tcgctcgtgc tcccgctggt cgcgggtctg aacgcggtcg
     1861 gcctggccgc gatctaccgg ctgtcgccga agggcttcgg cgcgacccag gtgacgtgga
     1921 ccgcgctcgg gctcgcgtgc ttcgcgggca cgctgatcgt cgtccgcgac taccgggccc
     1981 tgtcgcgcta caagtacatc ctcggcttcg ccgggatcgc gctgctgctg cttcccatga
     2041 cgcccgtcgg cgcggtcgtc cacggagcgc ggttgtgggt gaggttcggc gggttccagt
     2101 tccagccggc cgagctgtcg aagatctgcc tcgtcgcgtt cttcgccgcg tacctggccg
     2161 agcgcaagga gctgctggcg gtcgctacca agcgcatcgc cggcttccac gtgccggact
     2221 tcaagcactt cggccccctg ctcgtcatgt ggggcgtctc gctcgccgtg atgttctacg
     2281 agcgtgacct cggctcgagc ctgctgttct tctcggtctt cctgctgatg ctctacgtcg
     2341 ctacctcgcg cgtggtgtac accgccttcg gcgcgctgct gttcgtgggc ggggcgatcg
     2401 tcggttacca ggtgttcacg cacgtgcaga gccgcgtgca ggtgtggctc gacgtcttcg
     2461 accccgagct gatccagggg gcgagctacc agctcgcgca gtcgctgttc gcgctggcga
     2521 ccggcggcct gttcggcacc ggcctcggcc agggccggcc ggacctgata ccggtagccg
     2581 agaccgactt catcttcgcg gtcgtcggcg aggagctggg cctgatgggt accgtcgcgg
     2641 tgctcttgac gttcctgctg ctcgtcggac gcgggctgcg ggtcgccctg cacgcacggg
     2701 acgacttcgg gcagctgctc gcagccgggc tcacgttcat cctcggcctg cagacgatca
     2761 tcatcctcgg cggcgtcaca cggctgatgc cgctgaccgg gatcacgctg ccgttcatgt
     2821 cgtacggcgg gtcgagcatc ctctcgaact tcatcctggt cgcgttgctc ttacggatct
     2881 ccgaccagtc cgagggcgcg cctccacccg cgcacaccgc agagatcttg atcgggggcc
     2941 gcctcggcgg gacctcagag cagagcgggg cccgcctcgg cgggaccccg gagcagagcg
     3001 ggggccgccc atgatgtcgg cgcagatccg caaggtcggg atcgggctcc tccttgcgtt
     3061 caccgcgttg ttcctccagc tcaactacat ccagatctgg gccgccgagg acatcgcgtc
     3121 gaacccgaac aacatccgcg cgctgatcgc ggagtactcg gtcaagcgcg gcgacatcga
     3181 gacccacgac ggcaagctcg tcgcgacgtc ccgggagtcg aagggccgct acaagtacga
     3241 gcgcgtgtat cccgagggcg acctctacgc gcacatcgtc ggctacaagt cgatcgtcta
     3301 cggcgagacg cggctagagg ctgcgttcga cgagcagctg caaggaaaga ccgcggtcca
     3361 gtcgatgcag gacctcgagg acacgctcag cggcggcgag gagcagggcg acgacctccg
     3421 tctgacgatc catcaccagc tgcaggagaa ggcgcgcgac ctgctcgacg ggagagacgg
     3481 cgcgatcgtc gccctcaacc cgcagacggg cgaggtccgc gcgatgtgga gcaacccgtc
     3541 gtacgacccg aacccgctcg cgtcgcacga cacgaaggag gtccgccgct accggctgac
     3601 gctcgacccg aactcgaaca cgagcccgct gatcagccgc gcgacgtcgc gcagcttcgc
     3661 gccggggtcg acgttcaagg tcgtgacgac ggccgctgcg ctcgagtccg ggcagtaccg
     3721 tgcgaactcg acgttcccgg acccggtcgc gctcgatctt ccccagacgg acgacacgtt
     3781 gcagaacttc acgcgcacgg cctgcacgag cgccggacgg atcgacctgt tcacggccct
     3841 agaggtgtcc tgcgacacga cgtacgggat cctcggcatg gagatctacg accagctgcg
     3901 cgagacctcg gaggcgatgg gcttcaacga ggccatcccg ttcgacgtcg gcaccgaggc
     3961 cagcacgttc cccgacgtcg gcgacgacaa catcccgttc cgcgcgttcg ccgggatcgg
     4021 gcagggtgac gtgtccgcga ccccgctgca gatggcgctc gtcgcggcga cggtagcgaa
     4081 cggaggcgaa gtcccgcggc cccgtctcgt gcgtcaggtg atcgacccca gcggcggcat
     4141 cgtcgagccg ttcacgcccg agacgatcgg ccgcgcgatg tcgcaggaga ccgcggcgcg
     4201 cgtgaacgag atgatgcagg cggtcgtcct cagcgggacc gggacgagcg cgcggatcga
     4261 aggcgttccc gtcgcgggca agaccgggac cgcccagacc gtccccggcg agagcccgca
     4321 tgcgtggttc atcgcgttcg ctcccgcaga cgaccctcag ctcgccgtcg cggtgatcgt
     4381 cgagagcagc tcggcgggat cggaggcgac cggtggcgcg atcgcggccc ccctggccaa
     4441 gcagatcctg gagacggacc gggcgatcag cgcggcatgg tagccggacg cgtcctcggc
     4501 gaccgctacc ggatcgagga acggatcgcg gccggcggga tgggcacggt ctacgccgcc
     4561 accgacgacc gactgcaccg gcgcgtcgcg gtgaagctcc tgaaggaggg cctgtcgggg
     4621 gacgccaagt tcgtcgcgcg cttcgagcgc gaggcgcgtg cggccgccgc tctgtcgcac
     4681 cccaacgtcg cgggcgtctt cgactacggc gaggacggcg agacgccgtt catcgtcatg
     4741 gagctcgcgc agggacgcga cctcgcgcgg ctgttgcgcg acgaaggacc gctcgagccc
     4801 gaccgcgccc gggggctggg ggcccagata tgtaccgctc tggcacacgc ccatgccgcc
     4861 gggctcgtgc accgcgacgt caagcccgcg aacgtcatcg tcgacgagtc cgaccgcgtg
     4921 aaggtgaccg acttcgggat cgcgcgggcc gcgggggaag cgacgctcac cgcgaccggg
     4981 tccgtgctcg gcagcgcgca gtacatggcg cccgagcagg cttcgggcgc gcccgtgact
     5041 gcggcggccg acgtctacgc ggccgggatc gtgctgtacg agatgctcac gaacgcggtc
     5101 ccgttcaccg gcgactcgcc ggtctcggtc gcgatgaggc acgtgtccga cgaggtgccc
     5161 cggccgagct cggtcaaccc gcaggtgccc gagtcgctcg acgcgatcgt cgctcgcgcc
     5221 acggcgaagg accccgcgga tcgcttcccg agcgcggccg cgatgggggc cgcgctcgaa
     5281 ggagacgagg tgacggcgcc ggtcgccgcg ggcgaggcga cccaggtcat cacctcgtcc
     5341 ggcgctgccg cggagacttc cgtgtggcct atccccggca acaggtacga ccctcggcgg
     5401 ctcgggaccc gcgtcctcgc cgttgccgcg atcctggcta tcgccgctgt cgtcacctgg
     5461 gggtggctgc tctcacgaag cggtgacgcc gtcagggacc ggcgccgggc ccccgccgcg
     5521 gcgcggccaa cccagcagcc cgcgcaagcc ggcgcaaccg aaccgaccga accggcgtct
     5581 gacggatacg agctgccgag cttcaccggg atgccgttca aggaggcgga gaaggtcctc
     5641 gtcgagcagg ggatcgtggt cgtgcccctg ctgcagccga gcagcgagga gagggacgag
     5701 gtggtgggct ccgaacccgt ggccggaacc gtcgtcgcgg cgggtgacac cgttacgctg
     5761 ctcatcagcg acggtgccgg tggggacgag ggcgaggact ccgacgagga gggcccgggc
     5821 aactccgagg acgcgcccgg gcacgacaag gacgacaagg acaaggaaaa ggacaaggac
     5881 gaggagtgag cgccaccaac cgcacgtacg gaggtcgcta cgcggtcctc gagcccgtcg
     5941 ggtccggcgg gatggccgag gtgtaccgag cgcgcgacga gctcctcggc cgcgacgtcg
     6001 ccgtcaaggt cctgagcgcc cggctctcga gcgacaagtc gttcgtcgag cgcttccggc
     6061 gcgaggcgca gtcggccgcg aacctcaacc acccgaacgt cgtgtcgctg tacgactacg
     6121 gcgcggacga cggcgcctac tacatcgtca tggagtacat cgagggcaag tccctcggcg
     6181 acatcgtcgc cgagagcggg gccctgctcc ccgagcgcgc cgccgagatc gcatccgacg
     6241 tcgccgccgc tctcgagcgc gcccactcgt ccgggctcgt ccaccgcgac atcaagccga
     6301 cgaacatcat ggtgacgaac acgggccaga cgaaggtcac ggacttcggc atcgcccgcg
     6361 cgctcggcca gagcaccgag cagacccaga tgacccagac cgggatggtc ataggcacgg
     6421 ccgcgtacct ctctcccgag caggcacagg ggaaccccgt cgacgcacgc tcggacgtgt
     6481 actcgctcgg gtgtgtgctg tacgagatgc tcaccggccg gccgccgttc gccggcgacg
     6541 caccgcttgc gatcgcgtac aagcacgtgc gcgaggaccc cgtcccgccg tcgacggtca
     6601 accccgacgt gccgcgagag ctcgacgcgg tgacgttgaa ggcactctcg aagaaccccg
     6661 gcaatcgcta cacgaccgcg gccgagatgc gtgaggacct gcagcggttc ctgaacggtc
     6721 agaaggtcct cgcgaccccg ctgatggccg ccgagaccgc cgtcgccgcg ccggtctcga
     6781 ccgggaccca ggtgctgcgg acgaccgaga ccgagtacgc gccgccgcca cccgagccgc
     6841 gccgcggggt ctggtacgtg ctggcggccc tgttgatcct cgccttgttc gggctgggag
     6901 cgtggttgct cgcgaacaac ttcctcgggg gcggtgacga ggtcgacgtc ccgaacgtcg
     6961 tcggcctcac cgaggaggac gcggtcgaca ggctcgagcg acgcgggttc gaggtcgacg
     7021 tcgaggagcg gccgagcgac aagcccgaag gcgacgtctt ccggcaggac cccgaccgcg
     7081 gcgagcaggc ggaagagggt tcgacggtca cgatcttcgt ctcgacgggc cccgaggctt
     7141 tcgaggtccc ggacctgcgc ggtgaggagc tccgcgacgc tcgccgcgag ctgaagcagg
     7201 ccgacctcac cgttggcacg atcacccggc aaccgagcga ggacgtaccg cccgacaccg
     7261 tcctcgacca gttcccgcgg cccccggccg aggtcaggcc gggagacgcg gtcgacctcg
     7321 tcgtgtcctc cgggcccgag gcgatccccg caccttcggt cgtcggccag accgaagacg
     7381 acgcgatcgc cgagatcgag gcgctggggc tgttcgcgga tccgatccag gtaccggacg
     7441 acgcgccggc gggcgaggtc atcggccagg atcccgctcc gggcacgccg atggaggagg
     7501 gcgacgtcgt cgcgatcacg gtctcgacgg ggcctgaggg cgagcccatg ccggacgtgc
     7561 gcggcgacga cgccgacacg gcggaagcct ttctcgagaa cgacttcggg ctgagcgtca
     7621 cgcaggagga gggcgcctgc gcggcgccgc cgggggcggt ctgcgaccag gacccgccgc
     7681 cggacacgct cgtggcgccg ggcgactcgg cgatcctcta cgtgcagccg ggcggggccg
     7741 ccctgccggg cgacgacctg ttcgcgttcc tgagcttgct cgcgctgttc gcctagccgg
     7801 gagcggcggc ggacacgaag ttcgcgagca gctgggggcc gcggtccgtg aggatcgact
     7861 cggggtggaa ctggacgccc tcgaccggca gctcccggtg gcgcacgccc atcacgacgc
     7921 cgtcggtcgc gcgggcggtg acctccagcg cgtcgggcca gccgtcgtcg tcgatcgcga
     7981 gcgagtggta gcgcgtcgcc tcgaacggcg acgggagccc cgcgaacacg ccggtgccgt
     8041 cgtgctcgat cggcgagacc ttgccgtgca tggggccgac gggggcccgg ttcaccgttc
     8101 cgccgaaggc cgcgccgatg cactggtggc cgaggcagac gccgaggacc gggacgcggc
     8161 ccgcgaacgc ctcgatggcg gcgatcgaca cgccggcttc ggccggcgtg ccggggccgg
     8221 gcgagatcac gacggcgtcg ggctcgacgg cgacgaggtc gtcgagggcg gcgtcgttgc
     8281 ggacgacgac cggctcggcg ccgagggagc cgaaagcctg ggcgaggttg tagacgaacg
     8341 agtcgtagtt atcgacgaga aggagcttca tcggctctat cctcgcgtct ctgatggacg
     8401 atttcgtcga ggagatcact cgcggcaacg tctcgggcgt caagatcgtg ctcgccaccg
     8461 tcgtcctcgc actcgccgtc tatcaggtcc tcctcatggc cgtcgggtac ggcaagctca
     8521 agctcccgtt cctccaggcc aaggccgcct cgttcgcgca ccggtcgatc ggcgacgcga
     8581 tcctcctcgt cacgctcgtc gtcgcgttca tgtgcatcgc ctacttcggc tacgaggacg
     8641 gcggcgagca cgccgccggc ggggagaccg gccgcgcgac cttgcacgtc atcggcggga
     8701 ccgcggtcgt ggcggtgctc gcggtcaagg tgctcgccgt gcgctggtgg catgcgctcg
     8761 gccgcttcct ccccgtgatc gggatggccc tgttcggcct cctcgccctg acctgggcta
     8821 cgtccgccgg cgactacttc ttcgggagct gacgatggcc gaacgcaagt acacgtacca
     8881 gctgatcggc tcgctgctag tcggcctgct gatcgtgatc gtcacgatct cgatcgtcac
     8941 gggaaagctc ggcccccgtc tcgaccggcg ggaagagctg cgcgagctcg aagacgtgcg
     9001 cgaagaacgc caggagcaga gggaagagcg gcgcgaagag ctaaacgacg actagaagtt
     9061 cgccggcagc agcttctccg acagcatcac gaggaccact gccgtcccga cggctaccgc
     9121 ggcccacagc aacagctcga gggtcctcga cttccacgag cgcttcatcg ggcaacctcg
     9181 cgctcgagct ccgcgtagac gatcagccgt tcggccgcgg agtacttcgg gttgcacgtc
     9241 gtcagaacga cctcgtactt cttcgacggg atcacgatcg cgtcggagtc cgccggcacg
     9301 atcctcttgt cggtgacccg gtacgtgaag tcaccccagc gggtctcgaa gtggatgggg
     9361 tcgcccatct cc
//
LOCUS       DASQDO010000197         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_20222, whole genome shotgun sequence.
ACCESSION   DASQDO010000197 DASQDO010000000
VERSION     DASQDO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20222"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VEV43_05700"
     CDS             <1..346
                     /locus_tag="VEV43_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23049.1"
                     /translation="RIRRMNAVRGSEHDDTITGGDGRNALSGESGDDHLEGAGNKDVL
                     YGSYGDDVLRGGSGRDDLSDDYGSDRFFGGAGDDVLTDSFRNEQGQDTMDGGRGHDRC
                     VKGEKTTRCEEP"
     gene            complement(343..1296)
                     /locus_tag="VEV43_05705"
     CDS             complement(343..1296)
                     /locus_tag="VEV43_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYP23050.1"
                     /translation="MAPRAIWKGSITFGLITIPVGLYSAIGRQDEKFDFHLLHEKDGT
                     RIHYERTCDKGHKDIDWDEIVKGYEYQKGKWVTITDEDLEALELESLRTIDVVTFAPH
                     EQIDPIFFDKSYYMAPEESAVKAYRLIVDALEDEKLVGVCKVAIREREHLAAMRVKDR
                     MLVLQTMHWPEEVRDTKFEQLKKRPRIDDRERKMARQLIQHLSSDFDPKQFKDEYHKA
                     LKKLIDKKIAGEEIVVEPEPEPTGEVVDLMEALRASVEAARQGKRPTPPKKKTPKASS
                     GDEDLSGLSKQELLDRAKKLDISGRSKMGKDELVKAIAKAS"
     gene            1389..1655
                     /locus_tag="VEV43_05710"
     CDS             1389..1655
                     /locus_tag="VEV43_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HYP23051.1"
                     /translation="MAKNHGPTVKNDRQYEALRKDGASKEKAARIANASANEGKSKVG
                     RRGGKSGSYEDWSKKDLYEKAKEVGIEGRSSMDKKELMSALRNH"
     gene            2035..2475
                     /locus_tag="VEV43_05715"
     CDS             2035..2475
                     /locus_tag="VEV43_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23052.1"
                     /translation="MNEGSRSIWTFRVAGTDQNGTSIGPVQIKLRGISIEGNVSEGDS
                     VRVSGRWRRGSIRADQVHNLTTGAEVRAKNYTGLKVAVAALMVVVLGGIAYFGITSAR
                     EADERREEIQRQQQQQQQEFQGGIPEGYCEAAEEAGLQPPQCAD"
     gene            complement(2460..>3408)
                     /locus_tag="VEV43_05720"
     CDS             complement(2460..>3408)
                     /locus_tag="VEV43_05720"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP23053.1"
                     /translation="FAGTPALGAIYGLTALASVFNAIDRPARSAMLPAMVAPGKIAAA
                     MALRQVVFQVTQIVGPLVGGLLIAGVGIGWAYVIDALTFVAALVALRWVPPAPPEGVV
                     EQSPLEAVREGLRFSFRTPFILSIFVIDLVAMIFGMPRAVFPALAERTFDGGAATLSL
                     LYASVSAGALAGALTSGWVTRVRAQGAAVLLAVTVWGVAITLAGLTLFSLALTMVFLA
                     VAGAADVFSAVWRGTMLQENTPDHLRGRVTAVNLMIVTGGPRLGDVEAGVAASLVGAP
                     ASVALGGIACLLGTAAVAAGFPALRRYRQRLPEEGQSAH"
BASE COUNT          603 a         1165 c         1161 g          479 t
ORIGIN      
        1 ccgcatccgg cggatgaacg cggtccgcgg ctccgagcac gacgacacga tcaccggcgg
       61 cgacggccgg aacgccctgt caggcgaatc gggagacgac cacctcgagg gagccgggaa
      121 caaggacgtc ctctacggca gctacggcga cgacgtgctc cggggtggga gcggacgcga
      181 cgatctgagc gacgactacg gctccgaccg cttcttcggg ggtgccggcg acgacgtgct
      241 gacggactcc ttccggaacg agcaaggcca agacacgatg gacgggggcc gcgggcacga
      301 ccgctgcgtg aagggcgaga agacgacgcg ctgcgaggag ccctagctag ccttcgcgat
      361 cgccttcacg agctcgtcct tgcccatctt cgagcggccc gagatgtcga gcttcttggc
      421 ccggtccagc agctcctgct tcgacagccc gctcaggtcc tcgtcgcccg acgacgcctt
      481 cggcgtcttc ttcttcggcg gggtcggcct cttgccctgg cgcgccgcct cgacggacgc
      541 gcgtagcgcc tccatcaggt cgacgacctc cccggtcggc tccggctcgg gctcgacgac
      601 gatctcctcg ccggcgatct tcttgtcgat cagcttcttg agcgccttgt ggtattcgtc
      661 cttgaactgt ttcgggtcga agtcggacga gaggtgctgg atcagctgac gcgccatctt
      721 ccgttcgcgg tcgtcgatgc gcggcctctt cttcagctgc tcgaacttcg tgtcgcgcac
      781 ctcctccggc cagtgcatcg tctggagcac gagcatccgg tccttcaccc gcatggcggc
      841 gaggtgctcg cgctcgcgta tggcgacctt gcacacgccg acgagctttt cgtcctcgag
      901 cgcgtcgacg atcagccggt aggccttcac cgccgactcc tcgggggcca tgtagtagct
      961 cttgtcgaag aagatcgggt cgatctgctc gtgcggcgcg aacgtcacga cgtcgatcgt
     1021 gcggagggac tcgagctcga gggcttctag gtcctcgtcc gtgatcgtca cccacttgcc
     1081 cttctggtac tcgtagccct tgacgatctc gtcccagtcg atgtccttgt ggcccttgtc
     1141 gcacgtgcgc tcgtagtgga tgcgagtgcc gtccttctcg tgcaggaggt ggaagtcgaa
     1201 cttctcgtcc tggcggccga tcgcggagta gagcccgacc gggatcgtga tcaggccgaa
     1261 cgtgatcgaa cccttccaga tagctctcgg ggccatgccc gagaagtacc cgcggcgatc
     1321 ccgcgaaaac ctcgtgtcca ccaggccggc ccggggaagg attccggcat cgagcggaag
     1381 gaggagagat ggcgaagaac cacggcccca cggtgaagaa cgaccggcag tacgaagcgc
     1441 tgcgcaagga cggcgcgtcg aaggagaagg ccgcgcgcat cgcgaacgcg tcggcgaacg
     1501 aaggcaagtc gaaggtcggg cgccgcggcg gcaagtccgg gagctacgag gactggtcga
     1561 agaaggacct ctacgagaag gcgaaggagg tcggcatcga gggccgttcg agcatggaca
     1621 agaaagagct catgtcggcc ctacgcaacc actgaggaac cggcagggcg cgtccgccac
     1681 tccaccacac cgaggggtcg cactccgtct ccaggacgta gcggcccatg acacgaggcc
     1741 gtcagcgagc cggagggcta ctacgacacc caacaggaag ccgtcgacgg gggccgcgag
     1801 ctggcgcgca agaacggcgt gaagctgtac gtgcacggcg aggacggact ggtccgggac
     1861 gagacgggcc ccgtgtaagg aacggaacac ccgcgtcgtc tttacttttc ggcccccgtc
     1921 gcggatgctc gaccggctgt ggcgacgaac caaccctcca tgtccggcgt cggccggtcc
     1981 gggcccgtga cggtcacggg tcaggtcagc tccgtccagc aacggacgga gtggatgaac
     2041 gaaggatccc gcagcatctg gacgttccgc gtggcgggca ccgaccagaa cggcacctcg
     2101 atcggccccg tccagatcaa gctccgcggg atctcgatcg agggcaacgt ctccgagggc
     2161 gactcggtga gggtcagcgg acgctggcgc cgaggctcga tccgggccga ccaggtccac
     2221 aacctcacga ccggcgccga ggtccgcgcg aagaactaca ccgggctcaa ggtcgcggtc
     2281 gcggctctga tggtggtcgt cctcggcggg atcgcgtact tcgggatcac gtccgcgcgg
     2341 gaagcggacg aacggcgcga agagatccag cgtcagcagc agcaacagca gcaggagttc
     2401 caaggaggga tccccgaggg gtactgcgag gctgccgagg aggccgggct ccagccccct
     2461 cagtgcgccg actgaccttc ttcgggcagg cgctgccggt accgccgtag cgcggggaat
     2521 cccgcagcca cggccgcagt cccgagcagg catgcgatac cccccagcgc gaccgacgcc
     2581 ggggctccga cgagcgacgc ggcgacgccg gcttcgacgt cgcccaggcg cggccccccc
     2641 gtgacgatca tcaggttcac cgcggtcacg cgaccgcgca ggtggtccgg ggtgttctcc
     2701 tgcagcatcg tgccgcgcca caccgcgctg aacacgtcgg ccgcgccggc gaccgctagg
     2761 aacaccatcg tcagcgcgag cgagaacagc gtgagtcccg cgagcgttat cgcaaccccc
     2821 cacaccgtca ccgcgagcag gacggccgcg ccctgagcgc gcaccctcgt cacccaaccg
     2881 gacgtcagcg cgccggcgag cgccccggcg gagaccgagg cgtacaggag gctcagcgtg
     2941 gccgctcccc cgtcgaacgt ccgctccgcg agggccggga acaccgcgcg cggcatcccg
     3001 aagatcatcg cgacgaggtc gatcacgaag atcgacagga tgaacggcgt gcggaacgag
     3061 aagcgcaggc cctcgcgcac cgcctcgaga gggctctgct cgacgactcc ctccggaggc
     3121 gccggcggaa cccaccgcag agcgacgagc gcggcgacga acgtcagcgc gtcgatcacg
     3181 taagcccagc cgatcccgac tccggcgatg aggaggccgc cgacgagagg gccgacgatc
     3241 tgcgtcacct ggaagaccac ctgacgcagc gccatggccg cggcgatctt gccgggcgcg
     3301 accatcgccg gcagcatcgc cgaacgagcc gggcggtcga tcgcgttgaa caccgacgcg
     3361 agcgcggtga gaccgtagat cgcgccgagc gccggcgtgc ccgcgaac
//
LOCUS       DASQDO010000198         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1121901, whole genome shotgun sequence.
ACCESSION   DASQDO010000198 DASQDO010000000
VERSION     DASQDO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121901"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1273)
                     /locus_tag="VEV43_05725"
     CDS             complement(152..1273)
                     /locus_tag="VEV43_05725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYP23054.1"
                     /translation="MIPVNSVSLTPEDLANVSEAVETGWISSSGRFIEEFETSWAAYC
                     GRRFGVAVSNGTTALQLAVAALRPEPGAEIVMPSFTIMSCALAALYNDCVPVLVDCDP
                     DTWCLDVDQVADKLSERTFAVMPVHMYGHPADMDPLTRMSAERGVHVIEDAAQAHGAQ
                     YLASGSEWTRCGGFGTVSSFSFYANKPVTTGEGGMLLTDDDEVAERARGLRNLAFRKD
                     RRFVHAELGFNFRLTNVQAALGVGQVARIEQIVARKREVARAYAARLGALETVQLPVE
                     REWARSIYWMFGLVVSEETGLDAAEVARRLAERGVETRPFFVGLHEQPVLTGRGLFAG
                     ETYPVTHRLARQGLYLPSGLGLSDEEVGRVCDAVEEVLG"
     gene            complement(1279..>1528)
                     /locus_tag="VEV43_05730"
     CDS             complement(1279..>1528)
                     /locus_tag="VEV43_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23055.1"
                     /translation="RHVHLPLERELVGTSEVVIVKQGRCEVDFYSDDKELLTTLEMTE
                     GDVMLLLRGGHGFRMTEDTVLFEVKQGPYTGLAEKERF"
BASE COUNT          238 a          570 c          487 g          233 t
ORIGIN      
        1 cctccggtcc cgcaccccag atcgagcacg ctcttcaccg cggcgccgga ccgggaccag
       61 acgtcctcca ggaggtcgca ctcggccgag tagtccttcg tccggtacac cgagtcgtag
      121 ccctgtgcgt agagggggcc gaacgcctcg ctcaaccgag gacctcctcg acggcgtcgc
      181 agacgcgtcc gacctcttcg tcgctcaggc ccaacccgct cgggaggtac aggccctgcc
      241 gcgcgagccg gtgcgtaacg gggtaggtct cgccggcgaa gaggcctctg ccggtcagca
      301 ccggctgctc gtgaagaccg acgaagaacg gccgggtctc gacgccccgc tcggcgagcc
      361 ggcgcgctac ctcggctgcg tccagcccgg tctcctcgga gacgaccagg ccgaacatcc
      421 agtagatcga ccgggcccac tcgcgctcta cggggagctg cacggtctcc agggcaccga
      481 gccgcgccgc gtacgcgcgg gcgacctccc tcttgcgcgc gacgatctgt tcgatgcgcg
      541 caacctgccc gactcccagc gccgcctgaa cgttcgtcag cctgaagttg aagccgagct
      601 ccgcgtgcac gaagcggcgg tccttccgga aggcgaggtt gcggaggccc cgggccctct
      661 ccgcgacctc gtcgtcgtcg gtgagcagca tgcctccctc accggtcgtg accggcttgt
      721 tcgcgtagaa gctgaagctg ctcacggtcc cgaagcctcc gcaacgcgtc cactcggagc
      781 cgctcgccag gtactgcgcg ccgtgggcct gcgccgcgtc ctcgatcacg tggacgccgc
      841 gctccgcgct catccgcgtc agagggtcca tgtcggcggg atggccgtac atgtggacgg
      901 gcatcaccgc gaacgtccgc tccgagagct tgtccgcgac ctggtccacg tcgaggcacc
      961 acgtgtcggg gtcgcagtcg acgagcaccg gcacgcagtc gttgtagagc gcggccagcg
     1021 cgcacgacat gatcgtgaac gacggcatga cgatctcggc ccccggctcg ggacgcagcg
     1081 ccgcgacggc gagctgcagc gccgtggtcc cgttcgagac cgccacaccg aagcgccgcc
     1141 cgcagtacgc cgcccacgac gtctcgaact cctcgatgaa gcgcccggac gacgagatcc
     1201 atccggtctc gaccgcttcc gagacgttgg ccaggtcctc cggcgtcaaa gacaccgagt
     1261 tgaccgggat caccgaggtc agaaccgctc cttctcggcg aggcccgtgt aggggccctg
     1321 cttcacctcg aacagcacgg tgtcctcggt catccggaag ccgtgccctc ccctcaggag
     1381 cagcatcacg tccccttcgg tcatctcgag cgtcgtcagg agttccttgt cgtcgctgta
     1441 gaagtccacc tcgcaacggc cctgcttgac gatcacgacc tcggaggtcc cgacgagctc
     1501 gcgctcgagc ggcaggtgca cgtgcctc
//
LOCUS       DASQDO010000199         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_755348, whole genome shotgun sequence.
ACCESSION   DASQDO010000199 DASQDO010000000
VERSION     DASQDO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755348"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VEV43_05735"
     CDS             <1..607
                     /locus_tag="VEV43_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HYP23056.1"
                     /translation="AMLYIATGRWPYIAVGVALFAAGSYVLYHLRDHIANRISIWLDP
                     WQDPYDSGFQLVQSIYAVGGGGLFGQGLGRGVLLSESGEPYIPFLETDFIFSAITHEL
                     GLAGAAAVVLLYLVFTYRGFRIAMLADDGFSKLLAAGLTATLAIQAFIIVGGVIGLIP
                     LTGITLPFVSYGGSSIVANFLLLGLLLIVSNHVNQAVERDR"
     gene            604..2085
                     /locus_tag="VEV43_05740"
     CDS             604..2085
                     /locus_tag="VEV43_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYP23057.1"
                     /translation="MNPQIRRLFFVFAALFTAVIAMGTYWLWRAPDLEARQGNPNEVV
                     RQLTTKRGLVYASDGKTILAENRRRKVQGRTWFLRRYPQRGLAAHVVGYSTIERSRIG
                     IEESLNDYLTGSNANLSTLVDRVENTLRGLTQEGNHVVTTLDARAQRVAMQALAGQCG
                     SVVAIQPSTGRVLAMASTPTYDPNLIERNFRRATQVAAPCEPTSPLLNRATQGPVFIP
                     GSTFKIVTAAAALDSPRWSPESTFEDPGYCIEYGKRVFNYADQAGPHVYGTVDFVEAV
                     ENSVNSVFCDLGKAMGARKLIDYAKRFGFYDNPPLETPEEERSPSGLYSKGKLFDPKD
                     ANQVDPGRFAFGQERLLVTPLQMAMVAATVANGGVLMEPHVVDRIVSPERDVITKTKP
                     DEIRRVMRAETAAALTPMMVQVVESGTGTAAQISGIEVAGKTGTAETGRSDENDTSFI
                     AFAPADDPDVAIAVFLQNQDGTGGSTAAPIAKTVLETLLRRTT"
     gene            2099..>2738
                     /locus_tag="VEV43_05745"
     CDS             2099..>2738
                     /locus_tag="VEV43_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYP23058.1"
                     /translation="MAVSDTLINTLFDGRYRILRKLGSGGMANVYLAEDEDLGRRVAI
                     KILNERYASDDSFTERFRREAKSAAGLSHPNIVSIYDRGEADGRPYIAMEVIEGRSLK
                     ELIIASGSLPIAQSLDYAKQILHALRFAHRNGIIHRDIKPHNILLGAEDRLKVADFGI
                     ARAGASQMTEVGSIMGTAQYLSPEQARGAPVTGASDLYSFWIVLYVMLTGKTP"
BASE COUNT          449 a          991 c          877 g          421 t
ORIGIN      
        1 cgcgatgctc tacatcgcga ccggccgctg gccgtacatc gccgtcggcg tcgcgctctt
       61 cgccgccggg tcgtacgtcc tctaccacct gcgcgaccac atcgcgaacc ggatctcgat
      121 ctggctcgac ccgtggcagg acccctacga ctcgggcttc cagctcgtcc agtcgatcta
      181 tgcggtcggg ggcggcgggc tcttcggcca gggcctcggg cgcggcgtgc tcctgagcga
      241 gagcggcgag ccgtacatcc cgttcctgga gacggacttc atcttctccg cgatcacgca
      301 cgagctcggc ctcgcgggcg ccgctgccgt cgtcctcctc tacctcgtct tcacctaccg
      361 gggcttccgg atcgcgatgc tcgccgacga cggcttctcg aagctcctcg cagccggcct
      421 gacggcgacg cttgcgatcc aggcgttcat catcgtcggc ggcgtcatcg gcctgatccc
      481 gctgaccggg atcacgctgc cgttcgtctc ctacggaggc tcgagcatcg tcgcgaactt
      541 cctcttgctc gggctgctcc tgatcgtctc gaaccacgtc aaccaggcag tggagcgaga
      601 tcggtgaacc cccagatccg ccgcctcttc ttcgtcttcg ccgcgctctt cacggctgtg
      661 atcgcgatgg ggacctactg gctctggcgg gcacccgacc tcgaggcgcg ccagggaaat
      721 ccgaacgagg tggtgcgcca gctgacgacc aagcgcggcc tcgtgtacgc ctccgacggg
      781 aagacgatcc tcgccgagaa ccggcggcgg aaggtgcagg ggcgcacctg gttcctgcgc
      841 cgctatccgc agcgcggcct cgccgcgcac gtcgtcggct actccacgat cgagcgttcg
      901 cgcatcggga tcgaggagtc cctgaacgac tacctgaccg gctcgaacgc gaacctctcg
      961 acgctcgtcg accgcgtcga aaacacgctg cgcggcctga cgcaggaggg aaaccacgtc
     1021 gtcacgacgc tcgacgcgag ggcgcagcgg gtcgcgatgc aggcgctcgc ggggcagtgc
     1081 ggctcagttg tggcgatcca gccctcgacc ggtcgggtcc tcgcgatggc ctcgacgccg
     1141 acctacgacc cgaacctgat cgagcgcaac ttccgccgcg cgacccaggt agccgcgccc
     1201 tgcgagccga cttccccgct cctgaaccgc gcgacgcaag ggcccgtctt catccccggg
     1261 tcgacgttca agatcgtcac ggccgcggcc gcgctcgact cgccgcgctg gagccccgag
     1321 agcacgttcg aggatccggg ctactgcatc gagtacggca agcgcgtctt caactatgcc
     1381 gaccaggcgg gcccccacgt gtacgggacg gtcgacttcg tcgaggcggt cgagaactcg
     1441 gtcaactcgg tcttctgcga cctcggcaag gcgatgggcg cccgcaagct gatcgactac
     1501 gccaagcgct tcgggttcta cgacaacccg ccgctcgaga cgccggagga ggaacgctcc
     1561 ccgagcggcc tctactcgaa gggaaagctc ttcgatccca aggacgcgaa ccaggtcgac
     1621 ccgggccggt tcgccttcgg gcaggagcgg ctactcgtga cgccactcca gatggcgatg
     1681 gtcgccgcga cggtcgcaaa cggcggcgtc ctgatggagc cgcacgtggt cgaccgcatc
     1741 gtcagccctg agcgcgacgt gatcacgaag acgaaaccgg acgagatccg tcgcgtcatg
     1801 cgcgccgaga cggcagccgc cctgacgccc atgatggtgc aggtcgtgga gagcggcacc
     1861 gggaccgccg cgcagatctc cggcatcgag gtcgccggca agacggggac cgccgaaacg
     1921 gggcgctccg acgagaacga cacgagcttc atcgccttcg cccccgcgga cgaccccgac
     1981 gtcgcgatcg cggtcttcct ccagaaccag gacggaaccg gcgggtcgac ggccgcgccg
     2041 atcgcgaaga ccgtcctcga gacgctcctg aggcggacca cctagtctgg atcagcgcat
     2101 ggccgtctcc gacaccctga tcaacacgct cttcgacggg cgctaccgca tcctgcgcaa
     2161 gctgggctcg ggcgggatgg cgaacgtcta cctggccgag gacgaggacc tcggccgtcg
     2221 cgtcgcgatc aagatcctga acgagcgcta cgcctcggac gactccttca ccgagcgctt
     2281 ccggcgcgag gcgaagtcgg cggccgggct ctcgcacccg aacatcgtct cgatctacga
     2341 ccgcggcgag gccgacggac ggccctacat cgccatggag gtgatcgagg gccggagcct
     2401 gaaggagctg atcatcgcga gcggttcgct cccgatcgcc cagtcgctcg actacgcgaa
     2461 gcagatcctg cacgccctcc gctttgccca ccgaaacggg atcatccacc gcgacatcaa
     2521 gccccacaac atcctcctcg gcgccgagga ccgcctgaag gtggccgact tcgggatcgc
     2581 ccgcgccggt gcgtcccaga tgacggaggt cggctcgatc atggggacgg cgcagtacct
     2641 ctcccccgag caggcgcgag gcgcgccggt cacgggcgcg tccgacctct actcgttctg
     2701 gattgtactc tacgtgatgc tcacgggcaa gacgccgt
//
LOCUS       DASQDO010000200         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_877348, whole genome shotgun sequence.
ACCESSION   DASQDO010000200 DASQDO010000000
VERSION     DASQDO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877348"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VEV43_05750"
     CDS             complement(<1..1198)
                     /locus_tag="VEV43_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23059.1"
                     /translation="MRSLAWFEARKVARNPLLWLGALLLFALAVLNAWGYWPTVPQDA
                     EYVHEGLVVLAPFALLAGAALGLRDETSGAANVVSSTPLAGRSRIVPARLLAVATVAL
                     LSCVVAFAATWTTSIVRGGHGVPDAFLVLDASLYIALAGAAGFGIGRLTGSRIMSLLA
                     APLLPAFAYFLQGRQTGRVTDPSWLLPSPVVPGYFGPLGYLPDVFAIHSVYLLGGLLV
                     LGGGIWIAVARREGAGSVHVPVAVTIAGSLTVVAAGAWLVTQPFEVHVMSAARSTWVE
                     IHSPRDYDLLPRTGASSSLTRFSGPADRALATECAEAEGIVACVFPEYGTEFARAVVR
                     DAAPLAEVASLEGIPRKLRMVPVTEESGVNQCAGGDDELLLTSTRWSREFEVFESTGE
                     AAFYCAI"
     gene            complement(1195..>1508)
                     /locus_tag="VEV43_05755"
     CDS             complement(1195..>1508)
                     /locus_tag="VEV43_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP23060.1"
                     /translation="GRQVVLSTHLVEDVAAICTSVVVLWGGRVAFHGTPQELRERAAG
                     RVWSTPEPAPGALTSWRTETGAYRVVAERSPGPGATELAPTVEDGYLLLTSGLGEAVS
                     A"
BASE COUNT          252 a          566 c          527 g          163 t
ORIGIN      
        1 agatcgcgca gtagaacgcg gcctcgcccg tcgactcgaa cacttcgaac tccctcgacc
       61 atcgggtcga cgtgagcagg agctcgtcgt cgcctcctgc gcactgattg acgccgctct
      121 cctcggtgac cgggaccatg cggagcttgc gcgggatgcc ttccagcgac gcgacctccg
      181 cgagcggagc ggcgtctcgc acgacggccc ttgcgaactc ggtcccgtac tcggggaaga
      241 cgcacgcgac gatcccctcg gcctcggcgc actcggtcgc cagcgctcga tcggccggcc
      301 ccgagaaacg cgtcagcgac gagctcgcgc cggtgcgcgg gaggaggtcg tagtcgcgcg
      361 gggaatggat ctcgacccac gtcgaccgag cggcggacat cacatgcacc tcgaagggct
      421 gggtgacgag ccacgccccg gccgcgacga ccgtgagaga ccccgcgatc gtgaccgcga
      481 ccggcacgtg caccgacccg gctccttccc gccgcgcgac ggcgatccat atgccgcccc
      541 cgaggacgag caggcccccg agcaggtaga ccgagtggat cgcgaacacg tcggggagat
      601 agccgagggg gccgaaatag ccgggcacga ccgggctcgg cagcagccac gacgggtccg
      661 tcaccctgcc cgtctgacgg ccctgcagga agtacgcgaa cgccgggagc aggggggccg
      721 cgagcaggga catgatgcgc gaccccgtca gccggccgat gccgaacccc gccgcccctg
      781 cgagcgcgat gtacaagctc gcgtcgagga cgaggaacgc gtccgggaca ccgtggcccc
      841 cgcggacgat cgacgtcgtc cacgtagccg cgaacgcgac gacgcacgac agcagcgcga
      901 cggtcgccac ggcgaggagc cgggcgggga cgatccggct ccggccggcc agcggcgtcg
      961 acgagacgac gttcgcggcg ccgctcgtct cgtcccgaag gcccaacgcg gcccccgcga
     1021 ggagagcgaa gggagcgagc acgacgagtc cttcgtgcac gtactcggcg tcctgaggga
     1081 ccgtcggcca gtagccccag gcgttgagga ccgcgagcgc gaacagcaac agcgcgccga
     1141 gccacagcag tgggttccgc gcgaccttgc gggcctcgaa ccaggccagt gacctcacgc
     1201 ggacactgcc tcgccgagcc cggacgtcag caacaggtag ccgtcctcga cggtcggggc
     1261 gagctcggtc gccccgggcc ccggcgaccg ttcggcgacg acgcggtacg cgcccgtctc
     1321 cgtccgccac gacgtgagcg cccccggcgc gggctccggc gtcgaccaca cgcgcccggc
     1381 cgcgcgttcg cgcagctcct gcggcgtccc gtggaacgcg acccggcccc cccacagcac
     1441 gacgaccgag gtgcatatcg ccgcgacgtc ctccacgagg tgcgtcgaca ggacgacctg
     1501 gcgaccgt
//
LOCUS       DASQDO010000201         9454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_999852, whole genome shotgun sequence.
ACCESSION   DASQDO010000201 DASQDO010000000
VERSION     DASQDO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999852"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VEV43_05760"
     CDS             complement(<1..655)
                     /locus_tag="VEV43_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP23061.1"
                     /translation="MPDALMVSSSFLPGRGGIETYLAELCTALAPRLAVLAPARRGRE
                     RIPEDLPHETRGYEGRLFVPGPRAARATVEAARSLGTDRVLFGTPWPQVLTAPRLSRA
                     GLRYASIVHGAELLVPAAIPAVRGRLLRALAGADLLLPVSLFTAQRLRDLLEGAGMRV
                     PPMAVLRARVDLERFHPGAGGDAARARYGIAPGAPMIVCLGRMVARKGLDRLIAALPE
                     "
     gene            complement(683..1372)
                     /locus_tag="VEV43_05765"
     CDS             complement(683..1372)
                     /locus_tag="VEV43_05765"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYP23062.1"
                     /translation="MKLIQPDSTIPSIGSLRSAATPAQRLHQTLQSLVKTVNDYDTYT
                     GGHSCRVATMSGDVGRVLGYAKSDIAFLQQAGLVHDIGKIGIPDKVLNKATPLTADER
                     YLVRLHPILGASILSRMPGMERLVPIVLHHHECWDGSGYPSGVAGVRVPLEARIILVV
                     DAFDAMTSNRPYGRVLTTEEALAELRAHSATQFDPRVVDAMHEAYRGGLLDERRRSSA
                     TVYLDNVAKAS"
     gene            complement(1479..4076)
                     /locus_tag="VEV43_05770"
     CDS             complement(1479..4076)
                     /locus_tag="VEV43_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23063.1"
                     /translation="MMRTRAVVALMALLTTLAPFARASAAQGCEGVAASRFYEMIQAP
                     QWAAGGGAITAYAVDPRNPERLLATNGASVMETTDGGCSWEQTFAVGLLPSLEVPVSS
                     LNATVEHIAIPQRNSNLTYLVVEEKAGPISRPHVIVSRDRGKTWAASDEGLPPLSGEA
                     VTIEPAPSDPDVAYLVVRELATGTDSVYATNDAGATWEQRATGEGASAGMAIDPLDVT
                     NVWFYGAGGLYHSVDGGRTRALQNRLSPPIPFFDVHHVPGKPARLMAYQGETQDFMRS
                     DDGGATWYPEGTPQGSAISLTHAPATGQMAMSVHNAVFRFEEPSFWIEITPAGIEEID
                     LQDLQADATSDPSVFGNAGTYIARYTGLGFEVKLPDVEVDPLPPIDTTGANLTPPKTR
                     LEMRPGSTRKVPYELTLPEHPTGLDVFFLVDVSDSMNPTIAGLRHGMNEIVEELGRSK
                     IDVQFGLGSYKDYPIPGYGSPVAGDYPYRLDRAIGPPDAEFTAALETLTSSGGGDLPE
                     SQLTGVYQAATGAGEPGFVDPGRGAGFRSDALQVIVHMTDAAFNNSPAHPSPSMDVVA
                     AELENKGIYHIGLAVWGKHGNFNAVRDLTSLSDASESNAPTPVDCNGDGTVDIVAGAP
                     LVCDIREEARAGVLNLAPAIVATVKAVSREVPVELEATGGGRILGSVTPEVYPRIDVI
                     RSASLGFDVTFNCPKLRKKTTEQVTLQAKVLEVPVASAEVDVVCKAAPAAALAPKPKE
                     EKPPPPIVPPPPAPAPAVVLVPPAPPAPIPEPIPGTQPNAQAQGAMADQEQEQTQLAF
                     VHAAAWKAELEEAVAEELALSDQSQRPRRGVPPAAVYATASLMALAYAFMSVSRTRTQ
                     AALQRVRRY"
     gene            complement(4164..5387)
                     /locus_tag="VEV43_05775"
     CDS             complement(4164..5387)
                     /locus_tag="VEV43_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23064.1"
                     /translation="MKKTRVTTAAATALSALLVLSVNAGARPSRAPLEGEGAGLKLVA
                     NLNTGSGTDMEFTTIKGHEYGFVASRTVANGVTGGLNVINVDKPEKPKLVAKLPCNLN
                     QGDVQISHDNKTVILAADAAGGPDSCLMVGQAGFMTIDISNPKKPKAIGTAVIPRGSH
                     NTTAHPTKPYVYNSDSDGGASRGEVQIWSIKNPAKPELVTTLQNLPHSPHDLSFNKDG
                     TRMITAARTAVQYYDTTDPENPQLISVTPCPGCQLAHDAKITPDGKWAIIGDEANGGG
                     TYPCPGGALYFFEIVNETVLVLRGVYEPNEVVFATEGQTAPGSCTSHVFDISPDSTKV
                     AISWYSAGTRYLDISNPAGATFGENTSPAGGVTELGWFIPSGASSWSSKFHKGPYIYS
                     NDINRGFDVYKIDSK"
     gene            complement(5509..6732)
                     /locus_tag="VEV43_05780"
     CDS             complement(5509..6732)
                     /locus_tag="VEV43_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23065.1"
                     /translation="MKVLATAAVAGLLTMVATGSAGANPNPNAGVQPAPLSGEGEGLK
                     LIKNIPTASGTDMEFATIKGREYGFVAARTTANSPNGGLYVIDVTKPEKAKVAGYLPC
                     NMTQMDIQLSHDKKTVIMAADGAGGPDSCLMVGQLGFMTVDISNPKKPKAIGSAINPR
                     GSHNTTAHPTKPYVYNSDSDLENPRGEIQIWSIKNPAKPELVNTVTSVGLSPHDISFN
                     KKGTQAVTAGIESVELFDTTDPENPTLQSVFQCPGCSITHDAKFTPDDAGLVIGDEGG
                     GGGTYPCPGGALYFYELQQGIVPVLTGVYEPNEVVLAQEGQTNVGPCTSHVFDFDQDG
                     SHLAISWYTAGTRYLDITNPVGPTFGAQGTGVIEAGWFIPDGGNSWSSKIHKGPYIYS
                     NDIARGFDIYKIVGK"
     gene            complement(6968..7663)
                     /locus_tag="VEV43_05785"
     CDS             complement(6968..7663)
                     /locus_tag="VEV43_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23066.1"
                     /translation="MNKKLLVSLTAAVAVFATLMPLGAAQAAKKGGPVVVGEDPAGDW
                     GDGSNTAAGDALGQDLVSATIGMADAKTVNFVIGLNSLPPTGGTPEATRYTWDFSVDG
                     TGLELDGKFTNYSRGACDPTAGSCPPPRDPGLQPFLLRGECTTEATPAMTLTLCQELA
                     KIQAIFDADAATITIPVPVEALGAKPGSKIVGMAGTFGGTISAAPAAFFTRSDFPMDQ
                     LFTTKTFTLPKKK"
     gene            7841..7984
                     /locus_tag="VEV43_05790"
     CDS             7841..7984
                     /locus_tag="VEV43_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23067.1"
                     /translation="MIRNVRKLAAVAVAGALLLPLASAPQSFAQKASKTPKFVDLKKV
                     PKP"
     gene            8087..8890
                     /locus_tag="VEV43_05795"
     CDS             8087..8890
                     /locus_tag="VEV43_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYP23068.1"
                     /translation="MSEFLAEEAKGYGFKTRILELQGGTPARTVRVVEAVKKGTKNPD
                     EWITFIAHYDTIASAGLTIQGAYDDGSGTNILRYFGKAFSKIKTNKSIALLWFDAEES
                     GLVASQLYADMLEKKDQQIAAAFGFDMVGIGYPAPYCICIYHGPAPEDALKALPIIDY
                     VNFDFLKFPEGDGGAANTEKWPFGTDPHVCSCGPNIRNSDESNFAEKGWFSMRWTGMR
                     TASDYPGYHQPWDTVPFMEVVAGSRENLEKGTENTFLSAYYSTFVVDNL"
     gene            8944..>9454
                     /locus_tag="VEV43_05800"
     CDS             8944..>9454
                     /locus_tag="VEV43_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HYP23069.1"
                     /translation="MRLRLIPREESFFDLFEEMAGKVQQGAEGLLDLLRDYRDLDIKA
                     GRVVDIEHEGDEITHEMIRRLNTTFVTPFDREDIHKLASNLDDVLDHVEAAAEYLQLH
                     KIERPLPQMLALAEILQKAAATTAVAMPNLRKMSNLDEYWVEINRLENDGDRAYRRTI
                     AELFSGDYKA"
BASE COUNT         1390 a         3111 c         3375 g         1578 t
ORIGIN      
        1 tctcgggcag agcggcgatc aggcggtcga ggcccttgcg cgccaccatg cggccgaggc
       61 agacgatcat cggcgcaccc ggtgcgatcc cgtagcgagc ccgggccgcg tcgcctccgg
      121 cccccggatg gaaccgctcg aggtccacgc gcgctcgcag gaccgccatc ggcgggacgc
      181 gcatcccggc cccctcgagc aggtcgcgga gacgctgcgc cgtgaacagg ctgacgggca
      241 ggagcaggtc ggcgccggcc agcgcgcgca gcagccgccc ccggacggcg ggtatcgccg
      301 cggggacgag gagctcggcc ccgtggacta tggaggcgta gcggagcccg gcgcggctca
      361 gccggggcgc cgtcagcacc tgcggccacg gtgtcccgaa cagcacccgg tcggtgccga
      421 gggaccgtgc ggcctcgacg gtcgcgcgag cggcccgggg gccgggcacg aacagccggc
      481 cctcgtagcc gcgggtctcg tgggggaggt cttcggggat gcgctcgcgc ccgcgtctcg
      541 ccggcgcgag gacggcgaga cgcggggcca gcgcggtgca cagctccgca aggtaggtct
      601 cgatcccccc gcgacccggc aggaaggaag acgagaccat caacgcatcc ggcacccgcc
      661 gattatcggg ggccggacgg cgtcagctgg ccttggctac gttgtcgagg tagaccgtcg
      721 ccgacgagcg gcggcgctcg tccagcaggc cccctcggta cgcctcgtgc atcgcgtcga
      781 cgaccctcgg gtcgaactgc gtcgcggagt gcgcccgaag ctcggccagg gcctcctccg
      841 tcgtcagcac ccggccgtag gggcggttgc tcgtcatcgc gtcgaaggcg tcgacgacga
      901 ggatgatccg cgcctccagg gggacccgga cgcccgcgac gccgctcggg tagccgctgc
      961 cgtcccagca ctcgtggtgg tggagcacga tcgggacgag ccgctccatc cccggcatcc
     1021 gcgacaggat gctcgcgccg aggatcgggt ggaggcggac gaggtagcgc tcgtcggccg
     1081 tgagcggggt cgccttgttc agcaccttgt cggggatccc gatcttgccg atgtcgtgga
     1141 cgagcccggc ctgctggagg aacgcgatgt cgctcttggc gtagccgagg acgcggccga
     1201 cgtcgccgct catggtggcg acccggcacg agtggccgcc cgtgtaggtg tcgtagtcgt
     1261 tgacggtctt gacgagcgac tggagggtct ggtggaggcg ctgggccggg gtggcggcgc
     1321 tcctgaggct cccgatcgac gggatggtgc tatcgggctg gatcaacttc acggaagccc
     1381 ttcctttcgc atagcgcctc cggggtgcga tccggaggaa ctgactagtt agaaggagct
     1441 atcggcagaa ttttccagta gctaaaatgc gcgctcgtct agtacctgcg gaccctctgc
     1501 agggcggcct gggtcctggt ccgggacacc gacatgaagg cgtaggcgag cgccatcagc
     1561 gacgcagtcg cgtagaccgc ggccggcggg acgccccgcc ggggcctctg cgactggtcg
     1621 gacagcgcca gctcctcggc cacggcctcc tcgagctcgg ccttccacgc cgcggcgtgg
     1681 acgaaggcca gctgggtctg ctcctgctcc tggtcggcca tggccccctg agcctgggcg
     1741 ttgggctgtg tccccgggat cggctcgggg atcggagccg gcggggcggg cggcaccagg
     1801 accaccgcgg gggcgggggc cggaggcggc gggacgatcg gaggcggcgg cttctcctcc
     1861 ttgggcttcg gcgcgagagc cgctgccgga gcggccttgc agacgacgtc gacctcggcc
     1921 gacgcgaccg gcacctcgag gaccttcgcc tggagcgtga cctgctcggt cgtcttcttg
     1981 cggagcttcg ggcagttgaa cgtgacgtcg aagccgagcg aggccgagcg gatcacgtcg
     2041 atcctcgggt agacctccgg ggtcaccgag ccgaggatgc gtcctccgcc ggtcgcctcg
     2101 agctcgaccg ggacctcgcg gctcacggcc ttgaccgtcg cgacgatcgc gggggcgagg
     2161 ttgagcacgc cggccctcgc ctcctcacgg atgtcgcaca cgagcggcgc tccggcgacg
     2221 atgtcgacgg tcccgtcgcc gttgcagtcc accggcgtcg gtgcgttcga ctcgctcgcg
     2281 tccgacagcg acgtgaggtc cctgaccgcg ttgaagttcc cgtgcttgcc ccagaccgcg
     2341 aggccgatgt gataaatccc cttgttctcg agctcggccg cgacgacgtc catcgacggc
     2401 gacgggtgcg cgggagagtt gttgaacgcc gcgtccgtca tgtggacgat cacctggagc
     2461 gcgtcggaac ggaaccccgc gccccggccg gggtcgacga agccgggctc acccgcgccg
     2521 gtcgcggcct ggtacacgcc ggtcagctgt gactccggca ggtcgccgcc gccgctggac
     2581 gtgagggtct cgagggccgc ggtgaactcg gcgtccggcg gtccgatggc gcggtcgagc
     2641 cggtaggggt agtcgccggc gaccgggctc ccgtaccccg ggatcggata gtccttgtag
     2701 ctgccgaggc cgaactggac gtcgatcttc gaccgtccga gctcctcgac gatctcgttc
     2761 atcccgtggc gcagcccggc gatcgtcggg ttcatgctgt cggagacgtc gacgaggaag
     2821 aagacgtcga ggccggtcgg gtgctccgga agcgtcagct cgtacggcac cttgcgcgtc
     2881 gagcccggtc gcatctcgag gcgcgtcttg ggcggcgtca ggttcgcgcc cgtcgtgtcg
     2941 atcggcggga gggggtcgac ctcgacgtcg gggagcttca cctcgaagcc gaggcccgtg
     3001 tagcgcgcga tgtaggtgcc ggcgttgccg aagaccgacg ggtccgaggt cgcgtcggcc
     3061 tggaggtcct ggaggtcgat ctcctcgatc cccgccggcg tgatctcgat ccagaagctc
     3121 ggctcttcga aacggaacac cgcgttgtgc acggacatcg ccatctggcc ggtcgcgggg
     3181 gcatgggtca gcgagatcgc gctgccctgt ggcgtcccct ccgggtacca ggtcgcgccg
     3241 ccgtcgtcgc tgcgcatgaa gtcctgggtc tcgccctggt acgccatcag gcgcgccggc
     3301 ttgccgggga cgtgatggac gtcgaagaag gggatcggcg gcgagagcct gttctggagc
     3361 gcccgcgtcc ggcccccgtc gaccgagtga tagaggccgc cggctccgta gaaccacacg
     3421 ttcgtgacgt cgagcgggtc gatcgccatc cccgcggaag cgccctcgcc ggtcgccctc
     3481 tgctcccagg tcgcgcccgc gtcgttcgtc gcgtagacgg agtccgtccc ggtcgctagc
     3541 tcgcgcacga cgaggtacgc gacgtccggg tcgctcggcg cgggctcgat cgtcacggcc
     3601 tcgccggaca gcggaggcag gccctcgtcc gatgcggccc acgtcttgcc gcggtcgcgg
     3661 ctcacgatca cgtgcggccg gcttatgggc ccggccttct cctcgacgac gaggtacgtg
     3721 aggttgctgt tccgctgggg gatcgcgatg tgctcgacgg tcgcgttgag cgacgagacc
     3781 gggacctcga gggacggcag cagcccgacc gcgaacgtct gctcccacga gcagccgccg
     3841 tcggtggtct ccatgaccga ggccccgttc gtggcgagaa gcctctcggg gttccggggg
     3901 tcgaccgcgt aggcggtgat cgccccgccg ccggccgccc actggggggc ctggatcatc
     3961 tcgtagaacc gggacgcggc gacgccctcg cacccttgtg cggcgcttgc tcgggcgaag
     4021 ggtgcgagcg tcgtgagcag ggccatcaag gccaccacgg cccgcgttct catcacctgg
     4081 aaagggtacg acgcaggccc ggccgggcct gcgtcgtgaa gttggccgct ttgcccgtcg
     4141 ccggaggcga cgggctgcgc tgcttacttc gagtcgatct tgtagacgtc gaagccccgg
     4201 ttgatgtcgt tcgagtagat gtacggcccc ttgtggaact tcgacgacca gctgctggcg
     4261 cccgagggga tgaaccagcc gagctccgtg acgccgccgg ccgggctcgt gttctcgccg
     4321 aacgtggccc ccgccgggtt gctgatgtcg aggtagcggg tgccggccga gtaccaggag
     4381 atcgcgacct tcgtcgagtc cggcgagatg tcgaacacgt ggctcgtgca cgaccccggc
     4441 gccgtctggc cctccgtggc gaacacgacc tcgttcggct cgtacacgcc ccgcaggacg
     4501 agaacggtct cgttcacgat ctcgaagaag tagagggcgc cgccggggca cgggtaggtg
     4561 ccgccgccgt tggcctcgtc accgatgatc gcccacttgc cgtcgggcgt gatcttcgcg
     4621 tcgtgcgcca gctggcagcc ggggcacggc gtgacggaga tcagctgcgg gttctcgggg
     4681 tccgtcgtgt cgtagtactg caccgccgtg cgagccgccg tgatcatccg cgtgccgtcc
     4741 ttgttgaacg agaggtcgtg cggcgagtgc gggaggttct ggagagtcgt cacgagctcg
     4801 ggcttggccg ggttcttgat cgaccagatc tggacctcac cccgggacgc accgccgtcg
     4861 gagtccgagt tgtagacgta cggcttggtc gggtgtgccg tcgtgttgtg cgagccgcgg
     4921 gggatgacgg cggtgccgat cgccttcggc ttcttcgggt tcgagatgtc gatcgtcatg
     4981 aagcccgcct ggccgaccat caggcacgag tccgggccgc ccgccgcgtc cgcggccagg
     5041 atgacggtct tgttgtcgtg gctgatctgg acgtcgccct ggttgaggtt gcacgggagc
     5101 ttcgcgacga gcttcggctt ctcgggcttg tcgacgttga tgacgttgag cccgccggtc
     5161 acgccgttgg cgaccgtgcg cgaggcgacg aagccgtact cgtggccctt gatcgtcgtg
     5221 aactccatgt cggtgccgga gcccgtgttg aggttcgcga cgagcttgag gcccgcgcct
     5281 tcaccctcga gcggagcgcg gctcgggcgt gcgcccgcgt tcacggacag gacgaggagt
     5341 gcggagagag cggttgctgc tgcggtcgtt acacgtgtct ttttcatgta ctggttgttc
     5401 gacggtcttg caacgcttcc tgccgccgcg cgcggccaaa cgttgtcggg gcccggacgc
     5461 gcaagagccc cggggcgtgc ccggggctct tggtcggaac cgtgtccgct acttgccgac
     5521 gatcttgtag atgtcgaagc cgcgggcgat gtcgttcgag tagatgtacg ggcccttgtg
     5581 gatcttcgag gaccacgagt tgccaccgtc cgggatgaac cagccggcct cgatcacgcc
     5641 ggtcccctgg gcaccgaagg tcgggccgac ggggttcgtg atgtcgaggt agcgggtgcc
     5701 ggcggtgtac caggagatgg cgaggtgcga gccgtcctgg tcgaagtcga acacgtggct
     5761 cgtgcacggg ccgacgttcg tctggccctc ctgggcgagc acgacctcgt tcggctcgta
     5821 gacaccggtc aggaccggga cgatcccctg ctgcagctcg tagaagtaca gggcgccacc
     5881 ggggcacggg tacgtgccgc cgccgccacc ctcgtcaccg atcacgaggc cagcgtcgtc
     5941 cggggtgaac ttcgcgtcgt gggtgatcga gcagccgggg cactggaaca ccgactggag
     6001 cgtcgggttc tcggggtcgg tcgtgtcgaa gagctcgacg ctctcgatgc cggcggtcac
     6061 ggcctgcgtg cccttcttgt tgaacgagat gtcgtgcggg ctgaggccga cggacgtcac
     6121 ggtgttgacg agctcgggct tggcagggtt cttgatcgac cagatctgga tctcaccgcg
     6181 agggttctcg aggtcggagt ccgagttgta gacgtacggc ttggtcgggt gcgccgtggt
     6241 gttgtgcgag ccgcgggggt tgatcgcaga gccgatcgcc ttgggcttct tcgggttcga
     6301 gatgtcgacg gtcatgaagc cgagctggcc gaccatgagg cacgagtcgg ggccaccggc
     6361 accgtccgcc gccatgatga ccgtcttctt gtcgtggctg agctggatgt ccatctgcgt
     6421 catgttgcag gggaggtaac cggcgacctt cgccttctcg ggcttggtca cgtcgatcac
     6481 gtagaggcca ccgttcggcg agttcgccgt ggtacgggcg gcgacgaagc cgtactcgcg
     6541 gcccttgatc gtcgcgaact ccatgtcggt gccgctcgcg gtcgggatgt tcttgatcag
     6601 cttgaggcct tcgccctccc cggagagggg agcaggctgg accccggcgt tcgggttcgg
     6661 gtttgcaccc gcagagccgg tggcgaccat ggtgagcagg ccggcgacag ctgcggttgc
     6721 gaggactttc atcgacaacc ttctcatcgt tctccctttc ggaaatgggc ggtttcgttc
     6781 cgcactatct gttcggtggt gggtgattcg acggtgtggg ggccgttcct gcgcttgttt
     6841 gcgaggagct gacacggaga tgacgcgagc cgggtgtttt gtcctatttg aatccgacgg
     6901 ttcactcgaa cgcgggaaaa gaaaaacggc ccgtccgatc gacgggccgt ttttcgtact
     6961 ggtttgtcta cttcttcttc ggcagcgtga aggtcttcgt cgtgaacagc tggtccatcg
     7021 ggaagtcgct gcgggtgaag aacgccgcgg gggccgcgga gatcgtcccg ccgaaggtgc
     7081 cggccatgcc gacgatcttc gagcccggct tcgcaccgag cgcctcgacc gggacgggga
     7141 tcgtgatcgt cgcagcgtcg gcgtcgaaga tcgcctggat cttcgccagc tcctggcaca
     7201 gcgtgagcgt catcgcaggg gtcgcctcgg tcgtgcactc gccccggaga aggaacggct
     7261 gcaggcccgg gtcgcgcggg ggcgggcagc tgccggcggt cgggtcacat gcgccacgcg
     7321 agtagttcgt gaacttgccg tcgagctcga ggcccgtgcc gtcgaccgag aagtcccacg
     7381 tgtaacgggt ggcctcgggc gttccgccgg tcggcgggag cgagttcagg ccgatgacga
     7441 agttcaccgt cttggcgtcg gccatcccga tcgtcgcgga cacgaggtcc tgaccgagcg
     7501 cgtcgccggc ggccgtgttg ctcccgtcgc cccagtcgcc cgcggggtcc tccccgacca
     7561 cgaccggacc gcccttcttg gcggcctgcg cggcccccag cggcatcaac gtcgcgaaga
     7621 ccgccacggc ggccgtcagc gagaccagca gcttcttgtt catgtctttt ctcctgctct
     7681 cgttcgggtt tcgtccttga cgcggaagtt cgacggttcg gcgtgtggtc ctgcaaaccg
     7741 gccacgctct cccgcggcag gaacgacgcc ctcgcgaggc gaacaaggtc tcggaaggac
     7801 acgacaggac acgacccgac cgatttgggg gacccacacg atgatccgca acgtcaggaa
     7861 gctcgccgcc gtcgccgtgg ccggcgcgct gctgctgccg ctcgcgtcgg cgccgcagtc
     7921 gttcgcccag aaggcgtcga agaccccgaa gttcgtcgac ctgaagaagg tccccaagcc
     7981 gtagatcacg tgggccgaga tcatcaccgg gctcgaggag ctcgtcgacc agtacccggc
     8041 gcgctacaac ctgccgggcg tcggtcctgc cccccacaac atcgcggtgt ccgagttcct
     8101 cgccgaggag gccaagggct acggcttcaa gacccgcatc ctcgagctgc agggcgggac
     8161 gcccgcgcgt accgtgcgcg tcgtcgaggc cgtcaagaag ggcacgaaga acccggacga
     8221 gtggatcacc ttcatcgccc actacgacac gatcgcgagc gccggcctga cgatccaggg
     8281 cgcgtacgac gacggctccg gcacgaacat cctgcgctac ttcggcaagg cgttctcgaa
     8341 gatcaagacg aacaagtcga tcgcgctgct gtggttcgac gcggaggaga gcggcctcgt
     8401 cgcctcgcag ctctacgccg acatgctcga gaagaaggac cagcagatcg ccgcggcgtt
     8461 cggcttcgac atggtgggca tcggctaccc ggccccctac tgcatctgca tctatcacgg
     8521 ccccgccccc gaggacgcgc tgaaggcgct cccgatcatc gactacgtga acttcgactt
     8581 cctgaagttc ccggagggcg acggcggcgc ggcgaacacc gagaagtggc cgttcgggac
     8641 cgacccgcac gtctgctcct gcggcccgaa catccgcaac tcggacgagt cgaacttcgc
     8701 cgagaagggc tggttctcga tgcgctggac cgggatgcgg accgcgagcg actacccggg
     8761 ctaccaccag ccgtgggaca cggtcccgtt catggaggtc gtcgccggga gccgcgagaa
     8821 cctcgagaag ggcaccgaga acacgttcct ctcggcgtac tactcgacgt tcgtggtcga
     8881 caacctgtag gggttagcgc gctatcctcc ccggcgtagg cagagccgac cggggagagt
     8941 caattgcgtc tgcgcctcat tccgcgcgaa gaatcgttct tcgacctgtt cgaagagatg
     9001 gcggggaagg tccagcaggg ggccgaaggc ctgctggacc tcctgcgcga ctacagggac
     9061 ctcgacatca aggccggacg cgtcgtcgac atcgagcacg agggcgacga gatcacgcac
     9121 gagatgatcc ggcgcctcaa cacgacgttc gtgacgccgt tcgaccgcga ggacatccac
     9181 aagctcgcgt ccaacctcga cgacgtgctc gaccacgtcg aggccgcggc cgagtacctg
     9241 cagctccaca agatcgagcg cccgctgccc cagatgctcg cgctcgccga gatcctgcag
     9301 aaggccgccg cgacgaccgc ggtcgcgatg ccgaacctgc gcaagatgtc gaacctcgac
     9361 gagtactggg tcgagatcaa ccggctggag aacgacgggg accgcgcgta ccggcggacc
     9421 atcgccgagc tcttctcggg cgactacaag gcga
//
LOCUS       DASQDO010000202         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_755626, whole genome shotgun sequence.
ACCESSION   DASQDO010000202 DASQDO010000000
VERSION     DASQDO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755626"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1548)
                     /locus_tag="VEV43_05805"
     CDS             complement(163..1548)
                     /locus_tag="VEV43_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYP23070.1"
                     /translation="MKGQRVAAIFAATALIALGLSMGLLRAEDDPAPKTRRDEPEPLP
                     NVLIVITDDQRGDAMWVLPRTKRQFEQGGVRFTQAFATTPLCCPSRASIFTGMYAHNH
                     RVKTNADAHEMDPEKTLQFHLQRRGYRTAIFGKYLNGWRTAPPHFDEWAVIGTHRYRG
                     GWNVNGERTTIGSYSTTYIRRQALRFLEEAEADDDRPWLLILTPQAPHPPATPEERYA
                     DATLPVPRPNPAQQEQDLSDKPPYFRSIADPSDDAVAEARERQLRTLMSVDDLVAATF
                     ARVAGLGEERNTLAFYLSDNGFTWGEHNLFGNAESKNSPYEQSIRIPLFVKWPERMNG
                     SRVDDRLAANIDVAPTVFDAAGVEVDIPRDGRSLLRDWDRRYLLLERWRTQHAPMIPD
                     WAALWTGTEQYVEYSDGGTITARAYYDLVEDPWQLDNLLTDENPVNDARARHLHQELR
                     KVRFCRGPACP"
     gene            1993..3987
                     /locus_tag="VEV43_05810"
     CDS             1993..3987
                     /locus_tag="VEV43_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HYP23071.1"
                     /translation="MKLDTKRGHVVVENVRPQVDCGLFRAKAIVGDAVEVYAEIFRDG
                     PAVLKAVVRYRGPNDANEGSPRRSSSSGWLEAPLEHLGNDEWTGTFRPTEIGRWQYQV
                     EAWTDHFATWRSGFMKKVEAGQDVALEVEEGARLLEARLKKVPAKERGPLNTAITAAR
                     DGDGPATGFGDPRVDALLDEGVGVLMARYPDRTGSTQSKPVFELQVDRERARFGAWYE
                     FFPRSTGKPGKHGTFKTAAKHLATVADMGFDVVYLPPIHPIGERFRKGKNNALEAQEG
                     DVGSPWAIGSKDGGHTDVNPQLGTIDDFDAFMKAADRAGIEVALDFAIQCSPDHPWVK
                     EHPEWFTIRPDGSIQYAENPPKKYQDIYPINFDTPDKDGLWTELKRVVDFWISHGVKI
                     FRVDNPHTKAFAFWHWLIDSVQEENPDVIFLAEAFTRPKVMRRLAKLGFTQSYTYFTW
                     RNTKLELQEYLTELTETELADYFRPNFFANTPDILHEYLQQGGPPAFKIRLVLASMLS
                     PTYGIYSGYELFENKPVRQGSEEYLHSEKYELRHRDLNVEGSLVPYVKRINDIRRTHT
                     ALSEMTNLTFHFVDNDNVMAFSKTSRDGDTILVVVNLNPVHWAEATVHLDLDALGIDP
                     AHPFRVHDLISDDTYLWHGSHNYVRLDPFHEPAHVFRVER"
     gene            4024..>4205
                     /locus_tag="VEV43_05815"
     CDS             4024..>4205
                     /locus_tag="VEV43_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23072.1"
                     /translation="MKTELGQPLLGGAAGAPSRVLANPQWYKTAVFYELYVRSFYDSN
                     ADGMGDFKGLLQKLDYL"
BASE COUNT          725 a         1461 c         1369 g          650 t
ORIGIN      
        1 ggtacggggg gcaggggccg cgtcgaccgg agcggccagg gcgagcgtcg agcacagcaa
       61 gaaccagacc ttgcgccgca tgggagcctc ctcgtcgacg taccgtcgac gggaggtgtt
      121 gtgtgtaaca agctcattac ctctgagtag atgtcttcaa cttcacgggc acgccggccc
      181 ccggcagaac cgaaccttcc gcagctcctg gtgcagatgc cgggcccgcg cgtcgttgac
      241 cgggttctcg tccgtcagga ggttgtccag ctgccagggg tcttccacca ggtcgtagta
      301 cgcccgcgcc gtgatcgtgc cgccgtcgga gtattcgacg tactgctcgg tccccgtcca
      361 cagcgcagcc cagtccggga tcatcggggc gtgttgcgtc cgccatctct cgagcagcag
      421 gtaccggcgg tcccagtcgc gtagcagcga ccggccgtcc cgcgggatgt cgacctcgac
      481 ccccgccgcg tcgaacaccg tcggagcgac gtcgatgttc gccgccagcc ggtcgtcgac
      541 gcggctcccg ttcatccgct cgggccactt cacgaacagc gggatccgga tcgattgctc
      601 gtacgggctg ttcttcgact cggcgttgcc gaacaggttg tgctcacccc aggtgaaccc
      661 gttgtccgac aggtagaagg ccagcgtgtt ccgctcctcg ccgagccccg ccaccctggc
      721 gaacgtcgcg gcgacgagat cgtccaccga catcagagtc cggagctgcc gttcccgcgc
      781 ctcggctacc gcgtcgtcgg acgggtcggc gatcgaccgg aagtagggcg gcttgtcgga
      841 caggtcctgc tcctgctgcg caggattggg acgcgggacg ggaagcgtcg cgtccgcgta
      901 tctctcctcc ggggtagccg gcggatgcgg tgcctgcggc gtgaggatca acagccaggg
      961 ccggtcgtcg tcggcttccg cctcctcgag gaaacgcagc gcctggcggc ggatgtaggt
     1021 cgtcgagtag gagccgatcg tcgtccgctc gccgttcacg ttccacccac ctcggtagcg
     1081 atgggtcccg atcaccgccc actcgtcgaa gtgcggcggt gccgtgcgcc agccgttgag
     1141 gtacttgccg aagatcgccg tgcggtaccc ccggcgctgt aggtggaact ggagcgtctt
     1201 ctccgggtcc atctcgtgcg cgtcggcatt ggtcttcacg cggtggttgt gggcgtacat
     1261 gccggtgaag atcgacgccc gcgacgggca gcagagcggt gtggtggcga aagcctgggt
     1321 gaaccggacg ccgccttgct cgaactgacg cttggtcctc ggcaggaccc acatcgcgtc
     1381 cccgcgctgg tcgtccgtta tgacgatcaa cacgttcggc aacggctcgg gctcgtctcg
     1441 ccgcgtcttc ggcgccgggt cgtcctcggc gcgcagcagt cccatcgaca ggccgagcgc
     1501 gatgagggcc gttgcagcga agatcgccgc gaccctttgc cccttcaccc ccacccccga
     1561 ttgctggctc cgtcgtccct aggcgaccgt atgggggccg gggggtgggt taccgctcag
     1621 ttctgagcac ttggacggat gcggcccgcg ctcatcggct caatcgacgg aggagcccgc
     1681 gccttccgac gcaagaagag atgcgttgcg accagcgcga cgactgcaca ccccgcgacg
     1741 gctccgaacc tcagggccgc ctcgaacggg cccatcgagg gattacgcac gtccgccggc
     1801 ggcgtcgagc aggacgacga ccgggaagag gaacccgagg aggctgagtc ccgctgcgat
     1861 ccccgtgctc gccactgccc tccgatcggt acgcccgaga cgccgcccgt cgagcagacg
     1921 ttagcctggc cggggcggcg ggcaaggtcg taaaaacgaa caaacgcggg caaccaatgg
     1981 agggattcgg tcgtgaagct ggacaccaag agaggtcacg tcgtcgtcga gaacgtccgc
     2041 ccccaggtcg attgcggcct cttccgggcg aaggcgatcg tcggcgacgc ggtcgaggtg
     2101 tacgccgaga tcttccgtga cgggcccgcc gtgctcaaag ccgtcgtgcg ataccggggg
     2161 cccaacgacg cgaacgaagg atcgcctcgg cgatcctcca gcagtgggtg gctggaggcc
     2221 ccgctcgaac acctcggcaa cgacgagtgg accggcacgt tccgcccgac cgagatcggc
     2281 cgctggcagt accaggtcga ggcctggacg gatcacttcg ccacgtggcg cagcgggttc
     2341 atgaagaagg tcgaggcggg gcaggacgtc gccctcgagg tggaagaagg cgcacggctg
     2401 ctcgaggccc ggttgaagaa ggtgccggca aaggagcgcg gccccctcaa caccgcgatc
     2461 acggccgcac gggacggtga cggacccgcg acagggttcg gcgatcctcg ggtcgacgcc
     2521 ctgctcgacg agggcgtcgg cgtcctcatg gcccgctacc ccgaccgcac cggctcgacc
     2581 cagtcgaagc cggttttcga gctccaggtc gaccgcgaac gtgcccgctt cggcgcctgg
     2641 tacgagttct tccctcgctc gaccggcaag cccgggaagc acggcacgtt caagaccgcg
     2701 gccaagcacc tcgcgacggt cgccgacatg ggcttcgacg tcgtctacct gccgcccatc
     2761 caccccatcg gtgagaggtt ccgcaagggc aagaacaacg cgctcgaagc ccaggagggc
     2821 gacgtcggca gcccctgggc catcggcagc aaggacggcg gccacacaga cgtcaacccg
     2881 cagctcggca cgatcgacga cttcgacgcg ttcatgaagg cggccgaccg ggccggcatc
     2941 gaggtcgccc tcgacttcgc catccagtgc tcgcccgacc acccgtgggt gaaggaacac
     3001 cccgagtggt tcacgatccg ccccgacggc tcgatccagt acgcggagaa cccgccgaag
     3061 aagtaccagg acatctaccc gatcaacttc gacacgcccg acaaggacgg gctgtggacg
     3121 gagctcaagc gcgtcgtcga cttctggatc tcgcacggcg tgaagatctt ccgcgtcgac
     3181 aacccgcaca cgaaggcgtt cgcgttctgg cactggctga tcgactcggt ccaggaggag
     3241 aaccccgacg tgatcttcct cgccgaggcg ttcacccggc cgaaggtcat gcgccggctc
     3301 gccaagctcg gcttcacgca gtcgtacacg tacttcacgt ggcgcaacac gaagctcgag
     3361 ctgcaggagt acctgaccga gctgacggag accgagctcg ccgactactt ccgcccgaac
     3421 ttcttcgcca acacgcccga catcctgcac gagtacctcc agcagggagg gcccccggcc
     3481 ttcaagatcc ggctcgtcct agcgtcgatg ttgtcgccga cgtacggcat ctactccggc
     3541 tacgagctct tcgagaacaa gccggtgcgg cagggctcgg aggagtacct gcactccgag
     3601 aagtacgagc tgcgccaccg ggacctgaac gtcgagggca gcctcgtccc gtacgtcaag
     3661 cggatcaacg acatccggcg cacgcacacc gcgctgtcgg agatgacgaa cctcacgttc
     3721 cacttcgtcg acaacgacaa cgtcatggcg ttcagcaaga cgtcgcgcga cggcgacacg
     3781 atcctcgtcg tggtgaacct caaccccgtc cactgggccg aggcgaccgt gcacctcgac
     3841 ctcgacgccc tcggcatcga cccggcgcac ccgttccggg tccacgacct catctcggac
     3901 gacacctatc tgtggcacgg gtcgcacaac tacgtgcggc tcgacccctt ccacgagccc
     3961 gcccacgtct tccgcgtgga gcgctaggtg ggagggaacc aaccggtacc tccgaagaca
     4021 gaggtgaaga ccgagctggg ccagccgctg ctcggcggcg ccgccggcgc gcccagccgc
     4081 gtgctcgcga acccgcagtg gtacaagacc gccgtcttct acgagctcta cgtgcgcagc
     4141 ttctacgact cgaacgccga cggcatgggc gacttcaagg gcctgctgca gaagctcgac
     4201 tacct
//
LOCUS       DASQDO010000203        13252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1122401, whole genome shotgun sequence.
ACCESSION   DASQDO010000203 DASQDO010000000
VERSION     DASQDO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122401"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VEV43_05820"
     CDS             complement(<1..1018)
                     /locus_tag="VEV43_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HYP23073.1"
                     /translation="MRVIRPGRHPDDRAILALAVPALGALAADPLYSVVDTILVGHLG
                     PRPLGALAIGTAAVPVSFWVFSFLAFGVTPKVAMLLAAGDRDRAGIVGVQALLLALSM
                     GAVVTVAGVLLAGPVVRGLGAGPEIAGLAEDYLRIRVLASMPVLLVTVGHGWLRGAQD
                     TKTPMLIVTAGAVANAVLDYLFIYPFGWGVAGAAWATVVGQAGAALAFVVVLRRRMRP
                     PSWRWHGPSLRELLRVGVALVIRTGVLLGALTVATSAAARMGVVPLAAWQIAMQVFSL
                     LAFTVDSVAIAAQALVGNRLGTGRPEEATAVGRRLVWWGLALGALLLVVLVPLARPLA
                     GAFTD"
     gene            complement(1116..3119)
                     /gene="uvrB"
                     /locus_tag="VEV43_05825"
     CDS             complement(1116..3119)
                     /gene="uvrB"
                     /locus_tag="VEV43_05825"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HYP23074.1"
                     /translation="MPPFKVVADFAPAGDQPTAIRGIAERVQRGERYTTLLGATGTGK
                     TATIAWALEQIQKPTLVLAPNKSLAAQLCNEFKQFFPHNAVEYFVSYYDYYQPEAYVP
                     SSDTYIEKDSSINDEIERLRHSATSALLQRNDVLIVASVSCIFGLGSPEEYLNQVVSV
                     ARGGTADRDFILQRLVDIQYERNDVNFVRGKFRVRGDTIEVFPAYEELALRIELFGDE
                     VDRIVQLDALTGEIVRELDHVTVYPASHYVASKSRMERAISAIERELADRLYVLENER
                     KLLEAQRLRMRTNYDLEMMREVGFCNGIENYSRHIEGREPGSRPNTLLDYFPDDFLVV
                     LDESHVAVPQLNGMYEGDQSRKRVLVEHGFRLPSALDNRPLRFDEFLEKVDQMVFMSA
                     TPGSFELRHGGEPVEQLVRPTGLIDPEVVIRPTKGQIDDLMEEIRKRTEAGARVLVTT
                     LTKKMAENLTDYLLESGIKVRYLHADIDTVERIEILRDLRLGEFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGYLRSQTSLIQTIGRAARNVEGQVIMYADEVTDSMRHAINETQ
                     RRRGIQMAYNAEHGIDPQSIRKAVSDILISGFGRKREGDTVPSKSKSRRRDPIGGTPD
                     DLKRLILQLQEEMHTAAEDLRFEYAARIRDEIADLKRELRDVG"
     gene            complement(3163..4548)
                     /locus_tag="VEV43_05830"
     CDS             complement(3163..4548)
                     /locus_tag="VEV43_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYP23075.1"
                     /translation="MRRTVRLLVTLAVLSTVLGAAPAPARGSCDDFEYDDKCEARSLL
                     VRSRVGIRVLPFLVPASDTLLAAGADYVEEESSGDIVLKAFDASTGRRRWSRSYGGPR
                     SEALTAGALGRKGRTLFVAGTKMRAGRTDWIVAAFSVRTGRVLWERTYGGGGHDAPGQ
                     ILFDGRNDAIYVAGGVSRRRPTAAVVAYDSSSGRRLWVRERRPYRREWIVLGVDLRAR
                     DLYVLERSSCRTSGCRKDGLTLTRAMPTRSGFHLEWSGPLSERDGSIVFGPSPLEATA
                     GGVIVGGVQYDEATYEVSFFERAWGRRRWTANPDPAGEPSVTYPILAADPRRRQVVVA
                     DASLGTPSKVRAIGLPDGRFQWERPFPGGDFPGTVYDVAVGRAGVYLAGMAEGRNETI
                     SPTTVHLGPDGGRRRWVARYAVGHGWEEGVGSYLRAGRHGVTVVLGTPTNDLGGFRDD
                     WVGHATYLNRE"
     gene            complement(4557..6305)
                     /locus_tag="VEV43_05835"
     CDS             complement(4557..6305)
                     /locus_tag="VEV43_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYP23076.1"
                     /translation="MEDTGSIRSQGGYDELSALYRIATLSTAYGNPAAVVEEILRIIE
                     QVVHCDRSVLFLYDEDADVLRLQDARDRGFVLPLSEPTILRRVLHSGLGEVVNDVDAD
                     SESNPIFADSLNARQLVAAPLVLTDKTLGVVGAVNSRRGAFTHEDLRLLTVLADRVAL
                     TIENGELVARLQRQVQELEGLHRLSRLLLSSETMERIVGESIRIVAEMLDCDKMALLL
                     NDDSGTCLVAMQSAVGLNEHQVEGLRITLDAPSLAATVFRTKTPLVSNDAPNDAWVNR
                     HMVELLGIESLLAVPLTTGPKPIGVLKAMNARKGYFSDDDVRFAMLLGGQVGALVESS
                     MGRQRERQLVQKLREADRTKSEFVSMLAHELKGPMTTILGFSETLKQQWDRVPDEKKL
                     SILDIVAKETGRLSRLVNDLLDVSRMEAGTLRYELNPVSLPELIDSIITVHTSLSAQH
                     LVVNDIPADLPKALADNDRLRQVFINLLTNATRYSPEGTAIRVRARHLPDEGTIEVSV
                     TDEGIGISDQDRDRIFSKFSMLPKPGWVKKGTGLGLFITKGIVEAHGGRIWVESEQGK
                     GSTFAFTLRAANGAPE"
     gene            complement(6309..7022)
                     /locus_tag="VEV43_05840"
     CDS             complement(6309..7022)
                     /locus_tag="VEV43_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYP23077.1"
                     /translation="MSESFEIVFLDAGETILYPHPSFSELFATVVTEAGVRVSAGDVE
                     DVRRRLAPHLVDMAEDSGADGGVSLSEESSRTFWTFLYRRFLQELGHEDEALVERLFS
                     VFSSTSTYRLYDDVLPALRELSERGYRLGLISNFERWLEEMLVELEVGHVFDVAVISG
                     IEGVEKPDASIYERALDKAGVDAGRAVHVGDSPGLDVAPARSVGIETVLLDRLGRYAD
                     HDGHRIASLKELPDLLSKL"
     gene            complement(7019..7618)
                     /gene="coaE"
                     /locus_tag="VEV43_05845"
     CDS             complement(7019..7618)
                     /gene="coaE"
                     /locus_tag="VEV43_05845"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004932549.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYP23078.1"
                     /translation="MLAVGLTGGIGAGKSTFAALLAERGAQVIDADQIGRDALAPGEP
                     AWHSVVSQFGDEILVPGAMQVDRKRLAGIVFEEPRKLAALNAIVHPVILQRIADDLEK
                     LSRTDAVVVVDAALITELGLRDALDAVVVVTTSRQERVDRLVRTRGMSLDDVNARMAS
                     QASPGELEANADYVVRNDGTLEDLAAEADRVWALLEERA"
     gene            complement(7627..9213)
                     /gene="rpsA"
                     /locus_tag="VEV43_05850"
     CDS             complement(7627..9213)
                     /gene="rpsA"
                     /locus_tag="VEV43_05850"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HYP23079.1"
                     /translation="MAEEQGGAAQASVDTIPDGSGGTIARTAPSTSGDTFAGGTIVEN
                     DVGESFEELMAAIEETIKPFDEGDLVSGTVVKIDKDEVLLDIGYKSEGVIPSRELSIR
                     NDVDPSEVVSTGDQIEALVLQKEDKEGRLILSKKRAQYERAWGKIEEIKNADGTVTGP
                     VIEVVKGGLIIDIGLRGFLPASLVEMRRVRDLQPYVGKELECRIIELDKNRNNVVLSR
                     RKFLEESQAEQRQDFLTSLAKGERRKGVVSSIVNFGAFVDLGGVDGLVHVSELSWKHV
                     DHPSEVVEVGQEVDVEVLDVDLERERVSLSLKATQEDPWRQFARQHEVGDVIEGRVTK
                     LVPFGAFVEVDDAIEGLVHISELADHHVERAEDEVGVHDRVPVKIIDIDLDRRRISLS
                     RKQAMRSDEAIGIAPETTRETAAPPEDTAEEAAPEAEMEMELEGGEGMDSSAAAVAVP
                     AGDGPTLEPDAVDAAPAQQEDDEPRLDPGIDEDASPTPAADGAAPGTDAPVASEDEGE
                     GGDASIESIVQDLKRERGQS"
     gene            complement(9627..9965)
                     /locus_tag="VEV43_05855"
     CDS             complement(9627..9965)
                     /locus_tag="VEV43_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23080.1"
                     /translation="MANLVISSTGAVPVVAAMMVSVAFAGGVWNVLTNSLRQAVTPDR
                     LLGRVQSAHRLLSWGAVPFGTLLGGLAAHTLGLRAPFLISSFLLCALAIGAFLNSEHL
                     KPEYMAAPAR"
     gene            complement(10014..11006)
                     /locus_tag="VEV43_05860"
     CDS             complement(10014..11006)
                     /locus_tag="VEV43_05860"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP23081.1"
                     /translation="MRAAEEALTQIEEVSVSRPPGPPPPPPPPRVDDPRVESPGRVIH
                     LPEAEPARLGPDFMRVWAAAAISNAGDGVRITALPLLAAAVTRNPIAISGILLAGNLP
                     WLLCSLPAGAIVDRIDRRKLVSGVSAARALVMALLCAAVVFGEATLPALYAVAFLQGL
                     GEVFSDNAVFALVPSVVPRERLEDANGRLESVVQVTNQFAGPAAGGFLFAHAAGLPLF
                     LDAVSFLVMALLVRGVKLRSRPSVHREEEAPREKTSIRADIAEGLRWLRARPALRNLS
                     YIAALTNLALNGTFGIFVLFALEELSMSPTAFGILLSVEALGALGGSLLAARIR"
     gene            complement(11249..11680)
                     /locus_tag="VEV43_05865"
     CDS             complement(11249..11680)
                     /locus_tag="VEV43_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HYP23082.1"
                     /translation="MRHKTTIGAVAAALCLALWGTTVATAGTTMSTALVGSEERPGPG
                     DPDGHGFIELTLNQGKGEICFELTLSGIDAATAGHIHKAPAGEAGSVFVPLFGSEGPA
                     AKCVTATKAQVKEIRRNPEMFYVNIHTAAYPAGAVRGQLGD"
     gene            complement(11760..>13252)
                     /gene="polA"
                     /locus_tag="VEV43_05870"
     CDS             complement(11760..>13252)
                     /gene="polA"
                     /locus_tag="VEV43_05870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HYP23083.1"
                     /translation="MGVELAGLDLDTHIASYLLDPGAPGYAIEERARKYTGRELKAVD
                     GVDRSEESQGAFDLGVGEEAEAEDACLRALAIAEIAEAVRPELARLGMEDLYRTIEHP
                     LIPVLAHMEKTGIAIDLDYLEEMARDLDKRIGVLEAECYDLAGERVNLGSPSQLRVLL
                     YDKLGLKTTRRTKTGLSTDARALQQLVDQHPFVQKLLDYRELAKLKSTYVDALPPLTD
                     PADGRVHTTFDQTVAATGRLSSTNPNLMNIPVRTDLGKQIRRAFVAAPGYTLMSVDYS
                     QIELRVMAHLSKDPVLVEVFANDEDVHTATAMRVYDLDPSELKTMHRSVAKMVNYGLA
                     YGMGAPGLAERLNVPVEEAREIMDSYFEVFEGVAKFLDTVVTQAYADGFTTTMFGRRR
                     YLPELGSGNPRVRSIGERQALNAPIQGSAADIMKLAMIKVDAALAESRTRMILTVHDE
                     LVFEVPEGDEDAAAELVRREMTGVADMEVPLKVDVSFGTNWSETKD"
BASE COUNT         1980 a         4706 c         4516 g         2050 t
ORIGIN      
        1 cgtcggtgaa cgcgccggcg agcggccgcg cgagcggcac cagcacgacg agcagcaggg
       61 cccccagcgc gagcccccac cacaccagcc tgcggcccac ggcggtcgcc tcctccggcc
      121 gcccggtccc caggcggttg ccgaccagcg cctgcgccgc gatcgcgacg gagtccaccg
      181 tgaacgccag cagagagaag acctgcatcg cgatctgcca cgcggcgagc ggcaccacac
      241 ccatgcgcgc ggccgccgac gtagcgacgg tcagcgcgcc gagcagcacg ccggtccgga
      301 tcacgagcgc gacgccgacc ctcaacagct cgcgcaacga cggcccgtgc catcgccacg
      361 acggcggccg catccgccgc cgcagcacga ccacgaacgc gagcgcggcc cccgcctgcc
      421 cgaccaccgt cgcccacgcg gcccccgcga cgccccagcc gaagggatag atgaacaggt
      481 agtcgaggac cgcgttcgcg acggccccgg cggtcacgat cagcatcggc gtcttcgtgt
      541 cctgcgcacc ccggagccac ccgtgcccca cggtcacgag cagcaccggc atggacgcca
      601 gcacccggat cctcaggtag tcctcggcga gccccgcgat ctcgggcccg gcccccagcc
      661 cccgcacgac cggcccggcg agcagcaccc ccgccaccgt cacgacggcc cccatcgaca
      721 gcgcgagcaa cagcgcctgc acgccgacga tcccggcgcg gtctcggtcc cccgcggcga
      781 gcagcatcgc caccttcggc gtcaccccga acgcgaggaa cgagaagacc cagaacgaga
      841 ccgggacggc ggcggtcccg atcgccagcg cgccgagcgg cctgggcccg aggtgcccga
      901 cgaggatcgt gtcgaccacc gagtacagcg ggtcggccgc cagggccccg agcgccggga
      961 ccgcgagggc gaggatcgcc cggtcgtcgg gatgccgccc gggccggatc acgcgcatcc
     1021 ctcctccccc ggggcgtcag acatgtctga catgtcagac accctcccgc ggagacgtgc
     1081 gcgcgcggcc gggatcggag ccgggggcct accgctcagc cgacgtcccg cagctcgcgc
     1141 ttcagatcgg cgatctcgtc gcggatccga gccgcgtact cgaagcgcag gtcctcggcc
     1201 gccgtgtgca tctcctcctg cagctggagg atcagcctct tgaggtcgtc gggcgtcccg
     1261 ccgatcgggt cgcgccgacg gctcttggac ttcgacggca ccgtgtcgcc ctcgcgcttg
     1321 cgcccgaagc ccgagatcag gatgtccgac accgccttgc ggatcgactg ggggtcgatg
     1381 ccgtgctcgg cgttgtacgc catctggatc ccccgccggc gctgggtctc gttgatcgcg
     1441 tggcgcatcg agtccgtcac ctcgtccgcg tacatgatca cctggccctc gacgttgcgg
     1501 gccgcgcggc cgatcgtctg gatcagagac gtctgcgagc gcaggtagcc ctccttgtcg
     1561 gcgtcgagga tcgcgaccag cgacacctcg ggaaggtcca gaccctcgcg gagcaggttg
     1621 atcccgacga gcacgtcgaa ctcgccgagc cgcaggtcgc gcaggatctc gatgcgctcg
     1681 accgtgtcga tgtcggcgtg gagataacgg accttgatgc cggactcgag caggtagtcc
     1741 gtcaggttct cggccatctt cttcgtcagc gtcgtgacga gcacgcgcgc cccggcctcg
     1801 gtgcgcttgc ggatctcctc catgaggtcg tcgatctgtc ccttcgtcgg gcggatcacg
     1861 acctcggggt cgatcaggcc cgtcggccgg acgagctgct cgacgggctc gccgccgtgg
     1921 cggagctcga acgaccccgg cgtcgcgctc atgaacacca tctggtcgac cttctcgagg
     1981 aactcgtcga acctcagagg ccggttgtcg agcgcgctgg gaagacggaa gccgtgctcg
     2041 accaggaccc gcttgcggct ctggtccccc tcgtacatcc cgttcagctg cgggaccgcg
     2101 acgtgcgact cgtcgaggac gacgaggaag tcgtcgggga agtagtcgag cagcgtgttc
     2161 gggcgggacc cgggctcgcg gccttcgatg tggcgcgagt agttctcgat cccgttgcag
     2221 aagccgacct cgcgcatcat ctcgaggtcg tagttcgtgc gcatccgcag ccgctgggcc
     2281 tcgaggagct tgcgctcgtt ctcgagcacg tacagccggt cggcgagctc gcgctcgatg
     2341 gccgagatcg cgcgctccat acggctcttc gacgcgacgt agtgcgacgc ggggtacacc
     2401 gtgacgtggt cgagctcgcg cacgatctcg ccggtgagcg cgtcgagctg gacgatccgg
     2461 tcgacctcgt cgccgaacag ctcgatccgc agcgcgagct cctcgtaggc ggggaagacc
     2521 tcgatcgtgt cgccgcggac ccggaacttg ccgcgcacga agttgacgtc gttccgttcg
     2581 tactggatgt cgacgagcct ctgcaggatg aagtcgcggt ccgcggtccc cccgcgggcg
     2641 acgctcacga cctggttcag gtactcctcc ggcgagccga ggccgaagat gcacgacacg
     2701 ctggcgacga tcagcacgtc gttgcgctgg agcagcgcgc tggtggcgga gtgccggagc
     2761 cgctcgatct cgtcgttgat cgacgagtcc ttctcgatgt aggtgtcgga cgacgggacg
     2821 tacgcctcgg gctggtagta gtcgtagtac gagacgaagt actcgaccgc gttgtgcggg
     2881 aagaactgct tgaactcgtt gcagagctgg gccgccagcg acttgttcgg tgcgagcacg
     2941 agcgtcggct tctggatctg ctcgagggcc cacgcgatcg tcgccgtctt gcccgtcccg
     3001 gtggcgccga gcagcgtcgt gtagcgctcg ccgcgctgga cgcgctcggc gatcccccgg
     3061 atcgcggtgg gctggtcccc ggcgggggcg aagtcggcta cgaccttgaa cgggggcatg
     3121 ggggcagtct aagaccgccc tgcgacaaga atccccgggc tgttattcgc ggttgaggta
     3181 cgtcgcgtgg ccgacccagt cgtcccggaa gccgcccagg tcgttcgtcg gcgtgcccaa
     3241 cacgaccgtg accccgtggc gcccggcgcg caggtacgag ccgacgccct cttcccaacc
     3301 gtgtccgacg gcgtagcgcg cgacccaccg gcgacggccc ccgtcggggc cgaggtgcac
     3361 cgtggtcggc gagatcgtct cgttccggcc ctcggccatc ccggccaggt acacacccgc
     3421 gcgcccgacg gcgacgtcgt agaccgttcc cgggaagtct cctcccggga aggggcgctc
     3481 ccattggaag cgtccgtcgg gcaacccgat ggcgcgcacc ttggagggcg tccccaggga
     3541 cgcgtccgcg acgacgacct gccgtctcct ggggtcggcg gcgaggatcg gatacgtgac
     3601 ggagggctcg cccgcgggat ccggattagc cgtccacctg cgtcgccccc acgccctctc
     3661 gaagaacgag acctcgtacg tcgcctcgtc gtactggaca ccaccgacga tcactccccc
     3721 ggcggtcgcc tcgagcggcg agggcccgaa cacgatcgag ccgtcccgct cgctcagggg
     3781 gccgctccac tcgaggtgga agcccgacct ggtcggcatc gcacgtgtca gcgtcaggcc
     3841 gtctttccgg cagccggagg tcctgcacga gctgcgctcg aggacgtaga gatcgcgagc
     3901 gcgcaggtcg actccgagaa cgatccactc gcgccggtag ggccggcgct cgcggaccca
     3961 caggcgacgg cccgacgacg agtcgtatgc cacgactgcc gccgtcggcc tccgcctcga
     4021 taccccccct gccacgtaga tcgcgtcgtt gcgtccgtcg aagaggatct gccccggagc
     4081 gtcgtgcccc cctcccccgt aggtccgctc ccacagaacg cggccggtgc ggaccgagaa
     4141 cgcggcgacg atccagtcgg tacgcccggc gcgcatcttg gtcccggcca cgaacagcgt
     4201 ccggcccttc cgtcccagcg cgccggcggt gagagcctcg ctacgcggcc ccccgtacga
     4261 gcgcgaccag cggcggcgac cggtgctcgc gtcgaacgcc ttcaagacga tgtcgccgga
     4321 gctctcctcc tcgacgtagt cggcacccgc ggcgagcagg gtgtccgagg ccggcacgag
     4381 gaacgggagc acccggatac ccaccctgct cctcaccagc agcgaacggg cctcgcactt
     4441 gtcgtcgtac tcgaagtcgt cgcacgagcc gcgggccggc gcgggagcgg cgccgagcac
     4501 cgtcgaaagg accgcgagcg tcactaggag ccgcaccgtg cgtcgcatcg gcgttgctac
     4561 tccggggccc cgttcgctgc acgcagcgtg aacgcgaacg tcgagccttt gccctgctcg
     4621 gactcgaccc agatccggcc cccgtgtgcc tccacgatcc ccttcgtgat gaacagcccc
     4681 agcccggtcc ccttcttgac ccagccgggc ttcggcagca tcgagaactt ggagaagatg
     4741 cggtcgcggt cctggtcgga gatcccgatg ccctcgtccg tgacggacac ctcgatcgtc
     4801 ccttcgtccg ggaggtgacg agcgcgcacc ctgatcgcgg tcccctcggg cgagtagcgc
     4861 gtggcgttcg tgaggaggtt gatgaagacc tgacgcagcc ggtcgttgtc cgcgagggcc
     4921 ttcggaaggt cggcggggat gtcgttcacg acgaggtgct gcgcgctcag cgacgtgtgg
     4981 acggtgatga tgctgtcgat cagctcgggc agcgagacgg ggttgagctc gtagcgcagc
     5041 gtcccggcct ccatgcgcga cacgtcgagg aggtcgttga ccaggcggga cagccggccg
     5101 gtctccttcg cgacgatgtc gaggatcgag agcttcttct cgtcggggac cctgtcccac
     5161 tgctgcttga gcgtctcgct gaagccgagg atcgtcgtca tcgggccctt gagctcgtgc
     5221 gccagcatcg acacgaactc ggacttcgtc cggtcggcct cgcggagctt ctggacgagc
     5281 tgccgctcgc gctggcgccc catgctcgac tcgacgagcg cacccacctg gcccccgagg
     5341 agcatcgcga agcggacgtc gtcgtcgctg aagtagccct tgcgcgcgtt catggccttg
     5401 aggaccccga tgggcttcgg ccccgtcgtc agaggcaccg ccagcagcga ctcgatcccg
     5461 agcagctcga ccatgtggcg gttgacccac gcgtcgttcg gcgcgtcgtt cgagaccagc
     5521 ggggtcttcg tccggaacac cgtcgcggcg agagacggcg cgtcgagcgt gatgcgcagc
     5581 ccctcgacct ggtgctcgtt cagcccgacc gcggactgca tcgcgacgag acacgtcccg
     5641 ctgtcgtcgt tgagcagcag cgccatcttg tcgcagtcga gcatctcggc gacgatccgg
     5701 atcgactcgc cgacgatgcg ctccatcgtc tcgctcgaca gcagcagacg cgacagccgg
     5761 tggaggccct cgagctcctg cacctgacgc tggaggcgcg cgacgagctc gccgttctcg
     5821 atcgtcagcg cgacgcggtc ggcgagcacg gtcagcagcc tcaaatcctc gtgcgtgaag
     5881 gcaccgcgcc gcgagttgac cgccccgacg accccgagcg tcttgtcggt caggaccagg
     5941 ggggcagcga cgagctggcg ggcgttgagg gagtcggcga atatcgggtt gctctcggag
     6001 tccgcgtcga cgtcgttcac gacctcgccg agccccgagt gcagcacccg gcgcaggatc
     6061 gtcggctcgg acagcggcag cacgaagccg cggtcgcgtg cgtcctgcag cctcagcacg
     6121 tcggcgtcct cgtcgtagag gaacagcacc gaccggtcgc agtgcacgac ctgctcgatg
     6181 atccgcagga tctcctcgac gacggcggca gggttgccgt acgcggtgct gagcgtcgcg
     6241 atccggtaca gcgccgagag ctcgtcgtat ccgccctgcg accggatcga gccagtgtct
     6301 tccatcccct agagcttcga gaggaggtcc ggcaactcct tcaaggaggc gatgcgatgg
     6361 ccgtcgtggt ccgcgtagcg acccaagcga tcgagcagca ccgtctcgat gccgaccgac
     6421 cgcgccggcg cgacgtcgag cccgggcgag tccccgacgt ggaccgcccg tcccgcgtcc
     6481 acgcccgcct tgtccagcgc gcgctcgtag atcgaggcgt cgggcttctc gaccccctcg
     6541 atccccgaga tgacggcgac atcgaagacg tggccgacct cgagctcgac gagcatctcc
     6601 tcgagccacc gttcgaagtt cgagatcagc cccaggcgat agccgcgctc cgagagctcg
     6661 cgcagagcgg gcaggacgtc gtcgtagagc cggtaggtgg aagtgctcga gaagaccgag
     6721 aagagccgtt cgacgagcgc ctcgtcctcg tggccgagct cctgcaggaa gcggcggtac
     6781 aggaacgtcc agaacgtgcg ggagctctcc tcggacagcg acactccccc gtcggccccc
     6841 gagtcctcgg ccatgtcgac gaggtgcggg gcgagacgcc ggcgcacgtc ctcgacgtcg
     6901 cccgccgaca cccggacgcc ggcctcggtc acgaccgtgg cgaacagctc ggagaacgac
     6961 gggtgcgggt acaggatcgt ctcgccggcg tcgaggaaaa cgatttcaaa ggactcgctc
     7021 acgcgcgctc ctccagcagg gcccagaccc ggtcggcctc cgcggcgagg tcctctagcg
     7081 tcccgtcatt gcggacgacg tagtcggcgt tcgcctcgag ctcccccggc gaagcctgtg
     7141 acgccatacg cgcgttgacg tcgtcgaggc tcatcccgcg cgtgcgcacc aggcggtcga
     7201 cgcgctcctg ccgcgaggtc gtcacgacca ccaccgcgtc gagggcgtcg cgcagtccca
     7261 gctcggtgat gagcgcggcg tcgacgacga ccaccgcgtc cgtccgcgac agcttctcga
     7321 ggtcgtcggc gatcctctgc aggatcaccg gatgcacgat cgcgttgagg gccgcgagct
     7381 tgcgcggctc ttcgaacacg atcccggcga gcctcttgcg gtcgacctgc atggccccgg
     7441 ggacgaggat ctcgtcgccg aactggctca cgacggaatg ccaggccggc tcgccgggcg
     7501 cgagcgcgtc ccgtccgatc tggtcggcgt cgatcacctg ggccccccgc tcggcgagga
     7561 gcgcggcgaa cgtcgacttg ccggccccga tcccgccggt gaggcctact gccagcatct
     7621 agggagctag gactgcccgc gctcgcgctt gaggtcctgg acgatcgact cgatcgacgc
     7681 gtcgccgccc tcgccttcgt cctcggacgc gaccggagcg tcggtcccgg gcgcggcacc
     7741 gtcggcggcc ggcgtcggcg aggcgtcctc gtcgatcccc gggtcgagcc ggggctcgtc
     7801 gtcctcctgc tgggccggtg cggcgtccac ggcatccggc tcgagtgtcg ggccgtctcc
     7861 ggccgggacc gcgacggccg cggcagacga gtccatgccc tcgccaccct cgagctccat
     7921 ctccatctcg gcctcgggcg cggcctcctc ggccgtgtcc tctggcggcg ccgcggtctc
     7981 acgcgtcgtc tcgggcgcga tgccgatggc ctcgtcgctg cgcatcgcct gcttgcgcga
     8041 caggctgatc cggcggcggt cgaggtcgat gtcgatgatc ttcacgggca cgcggtcgtg
     8101 cacgccgacc tcgtcctcgg cgcgctcgac gtgatggtcg gcgagctcgg agatgtggac
     8161 caggccctcg atcgcgtcgt cgacctcgac gaaggcgccg aacggcacga gcttcgtgac
     8221 ccggccctcg atgacgtcgc cgacctcgtg ctggcgcgcg aactggcgcc acgggtcctc
     8281 ctgcgtcgcc ttgagcgaca gcgacacgcg ctcgcgctcg aggtcgacgt ccagcacctc
     8341 gacgtcgacc tcctggccca cctcgacgac ctcgctcgga tggtcgacgt gcttccacga
     8401 cagctcggag acgtgcacga ggccgtcgac accgccgagg tccacgaacg caccgaagtt
     8461 gacgatcgat gagacgacgc ccttgcggcg ctcgcccttc gcgagggagg tgaggaagtc
     8521 ctggcgctgc tcggcctgcg actcctcgag gaacttgcgg cgggacagca cgacgttgtt
     8581 gcggttcttg tcgagctcga tgatgcggca ctcgagctct ttgccgacgt acggctggag
     8641 gtcgcggacg cggcgcatct cgacgagcga cgcgggaagg aagccgcgca ggccgatgtc
     8701 gatgatcagc ccgcccttca cgacctcgat gaccgggccg gtgacggtgc cgtccgcgtt
     8761 cttgatctct tcgatcttcc cccatgcccg ctcgtactgg gctcgcttct tcgagaggat
     8821 gaggcgccct tccttgtcct ccttctggag gacgagggcc tcgatctggt ccccggtcga
     8881 gacgacctcg gacggatcga cgtcgttgcg gatggacagc tcgcgcgacg ggatgacgcc
     8941 ctcggacttg tagccgatgt cgaggagcac ctcgtccttg tcgatcttga cgacggtgcc
     9001 cgacacgagg tcgccctcgt cgaagggctt gatcgtctcc tcgatcgcag ccatcagctc
     9061 ttcgaacgac tcgcctacgt cgttctcgac gatggtgccg ccggcgaacg tgtcgccgga
     9121 ggtggaaggt gcggtccggg cgatcgtgcc gccggagccg tcggggatcg tgtccacgga
     9181 agcttgcgca gctccgcctt gctcttcagc catacggtcg acttcctctt gttcgggtcc
     9241 cttacatgac aaacgacccc cggaacgggg gccacaggag tataggggct gagccctgcg
     9301 cggtgccggg acgcgcttag ggccgggagg cacacacgcc gtactgcgcc tcccggccct
     9361 aagaagtgcg gtagtgctag ctccagctac gaccgagctt cccggtctcg acacggatgt
     9421 cgatcactgc gcacctcctc ctcgagcctc ccaattgctc agtgacagca gcctgtcaaa
     9481 tgcaacttgc ctcggcaagg tacgtgcggg ccattcttga gtacgcagtt atgactatct
     9541 tccgtgtggc ccgaaccggt ggtcggcgcg cgtttgggcg cgagtgcgcg cgcaagacgt
     9601 gtggcggttg gggtaaacca atcgagttaa cgcgcgggcg cggccatgta ctcgggcttg
     9661 aggtgctccg agttcagaaa cgcaccgatc gcgagcgcgc acagcaggaa cgacgagatc
     9721 aggaacggcg cgcgcagtcc gagagtgtgt gcggccaacc caccgagcaa cgtcccgaac
     9781 ggcaccgcgc cccacgacag cagcctgtgc gcgctctgca cgcggcccag cagtcggtcg
     9841 ggcgtgaccg cctggcgcag cgagttcgtc aggacgttcc acacgccacc ggcgaacgcg
     9901 accgacacca tcatcgcggc gacgaccggg accgcgcccg tcgacgagat caccaggttc
     9961 gccacgccgg cgactgcgag cgcgcccgcg atcgcgagcg ggatcccgac gagctagcgg
    10021 atgcgcgccg ccagcagcga ccctccgagc gcgccgagcg cttcgacgct gagcaatatc
    10081 ccgaacgcgg tcgggctcat cgagagctcc tccagcgcga acaggacgaa gatgccgaag
    10141 gtcccgttga gcgcgaggtt cgtgagcgcg gcgatgtagc tcaggttgcg cagggccgga
    10201 cgcgcgcgca gccacctgag gccctccgcg atgtccgcgc ggatgctcgt cttctcacgg
    10261 ggcgcctcct cttcccgatg gacgctcggt cgcgagcgca gcttgacccc gcgcacgagc
    10321 agggccatga ccaggaagga cacggcgtcc aggaagagcg ggaggcccgc cgcgtgggcg
    10381 aacaggaacc ctccggcggc cgggcccgcg aactggttcg tcacctggac gaccgactcg
    10441 agccgtccgt tcgcgtcctc gagacgctcg cgcgggacga ccgacggcac gagtgcgaac
    10501 accgcgttgt cggagaagac ctcgccgagc ccctgcagga acgcgaccgc gtagagcgcc
    10561 ggcagcgtcg cctcgccgaa cacgaccgcc gcgcacaaca gcgccatcac gagagcgcgc
    10621 gccgcgctga cgccggagac gagcttgcgc cggtcgatcc ggtcgacgat cgccccggcc
    10681 gggagcgagc acaggagcca gggcaggttg ccggcgagga ggatcccgga gatcgcgatc
    10741 gggttgcgcg tcacagcggc tgcgagcaaa ggcagcgcgg tgatgcggac gccgtcaccg
    10801 gcgttggaga tcgcggccgc ggcccacacg cgcatgaagt ccgggccgag ccgtgcaggc
    10861 tcggcctcgg gcaggtggat gacccggccg ggtgactcga cgcgcgggtc gtcgacgcgc
    10921 ggtggaggtg gtggcggcgg ggggccgggc gggcgcgaga ccgacacctc ttcgatctgc
    10981 gtgagcgcct cttcggctgc ccgcatccac tcgcctccgt cccggatccc ggttacgtcc
    11041 gtctggcaag acgctgtcac gccgcgcccg gacgggacaa ggtggatatg ggtggtttac
    11101 gaggtgatcc caaaggacta cacgcccgcc cggtgaggcc atgccgattc gtgccgcgcg
    11161 cgagtgcgat gacctagacc gggcttgcac gcggaaaggg ccgcccggtc gggcggccct
    11221 tttgctcttg cgtggtggct gtggccggtc agtccccgag ctggcctctc acggctccgg
    11281 ccgggtatgc cgccgtgtgg atgttcacgt agaacatctc ggggttccgg cggatctcct
    11341 tcacctgggc tttcgtggcg gtgacgcact tcgcagcggg gccctcgctg ccgaacaggg
    11401 ggacgaagac gctgccggcc tcgcccgccg gggccttgtg gatgtggccg gcggtcgctg
    11461 cgtcgatgcc ggagagcgtc agctcgaagc agatctcccc cttgccctgg ttgagcgtga
    11521 gctcgatgaa gccgtggccg tcggggtcgc ccgggccggg ccgctcctcg gagccgacca
    11581 gcgcggtcga catcgtggtc cccgccgtgg cgacggtggt gccccacagg gcgaggcaga
    11641 gtgccgcagc tacggctccg atggtggtct tgtgacgcat ggtccctccc agtccgcgaa
    11701 tccgacctaa ctctgggagt ttccgacatt ttgtggcaga tcgccagggg gttcttcggc
    11761 tagtccttgg tctccgacca attcgtcccg aaggagacgt cgaccttcag cgggacctcc
    11821 atgtcggcga cgccggtcat ctcccgccgc accagctcgg cggcggcgtc ctcgtcgccc
    11881 tcgggcacct cgaagaccag ctcgtcgtgc acggtcagga tcatccgcgt ccgggactcc
    11941 gccagcgccg cgtcgacctt gatcatcgcc agcttcatga tgtccgccgc cgagccctgg
    12001 atgggcgcgt tcagcgcctg gcgctcccct atcgaacgca cccgcgggtt gccgctgccg
    12061 agctccggca ggtagcggcg gcggccgaac atcgtcgtcg tgaacccgtc cgcgtacgcc
    12121 tgcgtcacga cggtgtcgag gaacttggcg acgccctcga agacctcgaa gtacgagtcc
    12181 atgatctcgc gcgcctcttc gaccggcacg ttcagcctct cggcgagacc cggcgcgccc
    12241 atcccgtagg cgagcccgta gttcaccatc ttcgccaccg accggtgcat ggtcttcagc
    12301 tcggacgggt cgaggtcgta cacgcgcatc gccgtcgcgg tgtgcacgtc ctcgtcgttc
    12361 gcgaacacct cgacgagcac cgggtccttc gagaggtgcg ccatcacgcg cagctcgatc
    12421 tgcgagtagt cgaccgacat gagcgtgtaa ccgggcgcgg ccacgaacgc gcgccggatc
    12481 tgcttgccga gatcggtgcg caccgggatg ttcatgaggt tcgggttcgt gctcgacagc
    12541 cgcccggtcg cggcgacggt ctggtcgaac gtcgtgtgca cccgtccgtc ggcggggtcg
    12601 gtcagaggcg gcagcgcgtc gacgtacgtg ctcttgagct tcgcgagctc gcggtagtcg
    12661 aggagcttct gcacgaacgg gtgctggtcg acgagctgct ggagcgctct ggcgtcggtc
    12721 gagagcccgg tcttcgtgcg ccgcgtggtc ttgagcccga gcttgtcgta gagcagcacg
    12781 cgcagctgcg acggcgagcc gaggttcacg cgctcccccg cgaggtcgta gcactcggcc
    12841 tcgagcacgc cgatgcgctt gtcgaggtcg cgcgccatct cctccaggta gtcgaggtcg
    12901 atcgcgatcc cggtcttctc catgtgcgcg aggacgggga tcagcggatg ctcgatcgtc
    12961 cggtagaggt cctccatgcc gagccgcgcg agctcgggcc gcacggcttc ggcgatctcc
    13021 gcgatcgcga gcgcgcgcag gcacgcgtcc tccgcttccg cctcctcgcc cacgccgagg
    13081 tcgaacgcgc cctgcgactc ctcggaacgg tcgacgccgt cgactgcctt cagctctcgc
    13141 ccggtgtact tgcgcgccct ctcctcgatc gcgtagccgg gggcgcccgg gtcgaggagg
    13201 tacgacgcga tgtgggtgtc caggtcgagc ccggcgagct cgacgcccat cg
//
LOCUS       DASQDO010000204         1966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1122477, whole genome shotgun sequence.
ACCESSION   DASQDO010000204 DASQDO010000000
VERSION     DASQDO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122477"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VEV43_05875"
     CDS             complement(<1..319)
                     /locus_tag="VEV43_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23084.1"
                     /translation="MRRHPVHLAFAAAALLTLGLAPPAAAGSAATRCLGAAATIIGTA
                     GRDKLQGTPGRDVIAGLGSKDEIHGLGGDDLLCGSGEGANAGVRDKLDTIYGGPGDDR
                     LDGG"
     gene            complement(357..1529)
                     /gene="thiI"
                     /locus_tag="VEV43_05880"
     CDS             complement(357..1529)
                     /gene="thiI"
                     /locus_tag="VEV43_05880"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HYP23085.1"
                     /translation="MDALVVHYHELGLKGRNRDFFERTLAQNLKRALRGTGYSKLRRG
                     FGRIVVDFEPEARVGLAAERAAKVFGVAYVGAGRRVEPELDAIGDTALELMLAEPFES
                     FAVRSRRTHSTCTHKSSEIQEVVGARIKEASGARVDLRNAEATAWVELFAGAGIVYRK
                     RLRGPGGLPAGTSGRMLALLSGGIDSPVAAWRMALRGADVQFLHFHGQPYSDPSSVRQ
                     AADLAQVLARYQMRAVLHLVPLGDVQREIVTECPANLRVILYRRMMMRIAERLATDLG
                     AQALVTGDSLGQVASQTIENIRTVDAAVPGAEVLRPLVGMDKQEIVDLATRIGTYEIS
                     TRKHQDCCVLFEPRSPATKTHPGQADAAEAGLDVETLIGKALSGIETQVYELEPPD"
     gene            complement(1537..1827)
                     /locus_tag="VEV43_05885"
     CDS             complement(1537..1827)
                     /locus_tag="VEV43_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYP23086.1"
                     /translation="MVNTVVLAICDVHQIPEAAQAIADLPEVSEVYSVAGDYDLVIMV
                     RVRRHEDLAKVVTEGILRVPGVERTQTLVAFKVFSRHDVESLFSLGFDEETV"
     gene            complement(1837..>1966)
                     /locus_tag="VEV43_05890"
     CDS             complement(1837..>1966)
                     /locus_tag="VEV43_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYP23087.1"
                     /translation="TLEEVGKEFNLTRERIRQIEAKALSKLRHPSTPGKLRDMVTI"
BASE COUNT          288 a          714 c          664 g          300 t
ORIGIN      
        1 tgccgccgtc gagacggtcg tcgccggggc cgccgtagat cgtgtccaac ttgtcgcgca
       61 cccctgcatt cgcaccttcg cccgacccgc agaggaggtc gtcgcccccg aggccgtgga
      121 tctcgtcctt cgagcccagg cccgcgatga cgtcgcgacc cggcgtgccc tgcagcttgt
      181 cgcggcccgc cgtcccgatg atcgtcgccg cggctccgag gcagcgcgtg gccgcactcc
      241 cggcggcggc agggggcgcc aacccgagtg tgagcaacgc tgcagcggcg aacgcgaggt
      301 gtaccgggtg ccgtctcatg tgcgtacgac ccccggcggc gtcgagtcgt tccctgtcag
      361 tccggcggct cgagctcgta gacctgcgtc tcgatccccg acagcgcctt cccgatcagc
      421 gtctcgacgt ccagccccgc ttccgccgcg tcggcctggc cgggatgcgt cttcgtcgcc
      481 ggcgagcgcg gctcgaacag cacgcagcag tcctggtgct tgcgggtcga gatctcgtac
      541 gtcccgatcc tcgtcgcgag gtcgacgatc tcctgcttgt ccatgccgac gagcggccgc
      601 aggacctcgg ctcccggaac cgccgcgtcg accgtccgga tgttctcgat cgtctgcgac
      661 gcgacctgac cgagcgagtc gcccgtgacg agtgcctgcg ccccgaggtc ggtcgcgagc
      721 ctctccgcga tacgcatcat catccggcgg tacaggatca cgcgcaggtt cgccggacac
      781 tccgtaacga tctcgcgctg gacgtcgccg agcggcacga ggtggagcac ggccctcatc
      841 tgatagcggg cgagcacctg cgcgaggtcc gcggcttgcc ggaccgagga cgggtccgag
      901 tacggctgcc cgtggaagtg gaggaactgg acgtcggctc cacgcagcgc catccgccac
      961 gcggcgacgg gggagtcgat cccgcccgac agcagcgcca gcatccggcc gctcgtaccg
     1021 gccgggagcc cgcccggccc ccgcaaccgc ttgcggtaga cgatccccgc gccggcgaac
     1081 agctcgaccc acgcggtcgc ctcggcgttg cgaaggtcga cccgcgctcc cgacgcctcc
     1141 ttgatgcggg cgccgacgac ctcctggatc tcgctcgact tgtgcgtgca cgtggaatgc
     1201 gtgcggcgcg accggacggc gaacgactcg aagggctcgg cgagcatcag ctcgagcgca
     1261 gtgtcgccga tcgcgtccag ctcgggctcg acacgccggc ccgcgccgac gtaggcgacg
     1321 ccgaacacct tcgcagctcg ctcggcggcg agcccgaccc gcgcctcggg ctcgaagtcg
     1381 acgacgatcc ggccgaaccc gcggcgcagc ttcgagtagc cggtgccgcg cagggcgcgc
     1441 ttgaggttct gtgcgagcgt gcgctcgaag aagtcgcggt tgcgtccctt cagaccgagc
     1501 tcgtgatagt gaacgacgag cgcgtccacc gaaacgtcag accgtctctt cgtcgaaccc
     1561 cagcgagaac agcgactcga cgtcgtggcg ggagaacacc ttgaacgcga cgagcgtctg
     1621 cgtccgctcg acgcccggca cgcgcaggat cccctcggtc acgaccttcg ccaggtcctc
     1681 gtgccggcgc acgcgcacca tgatcacgag gtcgtagtcg cccgcgaccg aatagacctc
     1741 gctgacctcg ggaaggtcgg cgatcgcctg agcggcctcg gggatctggt ggacgtcgca
     1801 gatcgcgagg acgacggtgt tgaccatcga gaagggctag atcgtgacca tgtcgcggag
     1861 cttgccgggc gtgctcgggt ggcgcagctt ggacagagcc ttcgcctcga tctgccggat
     1921 gcgctcacgc gtcaggttga actccttgcc gacctcttcg agcgtc
//
LOCUS       DASQDO010000205         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1122587, whole genome shotgun sequence.
ACCESSION   DASQDO010000205 DASQDO010000000
VERSION     DASQDO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122587"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VEV43_05895"
     CDS             complement(<1..250)
                     /locus_tag="VEV43_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23088.1"
                     /translation="MTTLDDLAAVALCGTEKLARGRQLSKALLKDWNVSSGSVWSTVH
                     AAVGPHDGLDFVQGGKLADVRERGLEEPEPLLAEFIQER"
     gene            complement(493..565)
                     /locus_tag="VEV43_05900"
     tRNA            complement(493..565)
                     /locus_tag="VEV43_05900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(530..532),aa:Lys,seq:ctt)
     gene            complement(601..3306)
                     /locus_tag="VEV43_05905"
     CDS             complement(601..3306)
                     /locus_tag="VEV43_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP23089.1"
                     /translation="MIRLDVPDELALSFVEEPVLDLYSWGPWRIPDGFLDSLGRAVQQ
                     AVADERLSGWAAEGGEAYRRPTNGVAETLDLMLGFLFGGCAIRSGYWGDMIWRVFDPF
                     LAQPFERRPSWNYLRTQSGAWRPPGWLLPFTAGDDPGRRRTAFEVLRSLVGVFDGLEP
                     VAARSAALARLYDERAGSEHDLHAPADALADEWAKAADERTVAALPELGGPVGYLAWA
                     LDGFLDVHPYLRAAVGDGDDEDVAIAQLVLAAGIQQVPAEIAVVASEDLLTRIEGHHR
                     RLRAGFAPETWRDTTARWLVRATIRGELAACRAWLDMAMRLTGALNGLPGRAMLPGGT
                     VWVPVSTFEWNVRALLANRPLPNALGTKFERPERATTDEAPATPPPVGEILGQPELAE
                     AVADAVASAGPVRMLVAGPAGSGKGLAVDLLADALKVRGFAQPPVWLPAAVVNERNAL
                     GAVELVRQEIDRCDGIRLLVIDGLDEVLAGGEAAEAVGSELLRALEARSGLNVVALCD
                     PGRASEVFTANPILARAFRAVRTNDFDRDTFHRVFLRKVERLGGAVDEATAAEAARRL
                     VDMRPFRNLRNGHLAAAFAAEAVARARARATASRPEVAVEDLPEDVTGREAYEDPFAE
                     LDSLVGLHQVKQEVGLLVAEAKAERLRRDAGIKVPPPTRHFAFTGNPGTAKTTVARLL
                     ARIYQSLDLLSSGHLVEVSRVDLVARYIGQTAPLVRAAVERALGGILFIDEAYALTPE
                     GSEEDFGHEAVATLVKLMEDHRGDLVVIVAGYEEDMDRFLASNPGLSSRFARRIGFPD
                     YTDDELVKIFTSIAERTGVSLGPGVDERVRDLLRTTPRGPGFGNGRHVRTMFERALGR
                     QALRLTAGEGPIDVAAVKTLLPEDLPEPEQAGPDLESTASGGQYL"
     gene            complement(3348..3427)
                     /locus_tag="VEV43_05910"
     tRNA            complement(3348..3427)
                     /locus_tag="VEV43_05910"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3389..3391),aa:His,seq:gtg)
     gene            complement(3467..>3669)
                     /locus_tag="VEV43_05915"
     CDS             complement(3467..>3669)
                     /locus_tag="VEV43_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP23090.1"
                     /translation="TFTAFFDLAFGLGAVSLGAVASAFGYAGAFRVAALVAVGGLALL
                     YGYARRDARMVPTPPAPMGGGA"
BASE COUNT          578 a         1341 c         1217 g          533 t
ORIGIN      
        1 gacgctcctg gatgaactcc gctaggaggg gttctggctc ctctagcccg cgctctctga
       61 cgtcggccag cttccctccc tgaacgaagt cgagaccgtc atgcggtccg acagcggcat
      121 gcacggttga ccacaccgat ccggaggaga cgttccagtc cttcaggagc gctttcgaga
      181 gctgtcttcc acgcgcgagc ttctccgtcc cgcacaacgc gacggcggcg agatcgtcca
      241 gcgtcgtcat tcggctcatg gcgccccctc ggcgcgcgtt tcggtcagcc cctcacccac
      301 acgatgtgcg cctcctcgtc gtacgcgccc acgatgagag tgctagtggc ctgcctcgtg
      361 tcttcctcgg cacctcttgg atatgcgcca tgtctaatcc ttcgcgcgca tgcggcctat
      421 ttgcggccta cattgtcttt tcggcaatgc gacgagaagc cagatatggc tgttgaccag
      481 ggcttttgag ggtgggtcgc cagggaatcg aaccctgaac ctacggatta agagtccgtt
      541 gctctgccaa ttgagctagc gacccgccgg gcaggatacc aacggcccgg gcccccgccg
      601 tcagaggtat tgccccccgc tcgccgtcga ctctaggtcc ggccctgcct gctccggctc
      661 gggcaggtcc tccggcagca gcgtcttcac cgccgctacg tctatcggcc cctcgccggc
      721 cgtcaaacgt agcgcctgcc ggcccagcgc ccgctcgaac atcgtcctca cgtgacgacc
      781 gttgccgaag ccggggccgc ggggcgtcgt ccggagcagg tcgcggactc gttcgtctac
      841 ccccggccct agcgacactc cggtgcgttc tgctatcgac gtgaagatct tcacgagctc
      901 gtcgtcggtg tagtcgggga agccgatcct gcgcgcgaag cgagacgaca ggccggggtt
      961 cgaagcgagg aagcgatcca tgtcctcctc gtagcccgcg acgatgacga ctaggtcgcc
     1021 gcgatggtcc tccatgagct tgacgagcgt cgcgacggct tcgtggccga agtcctcctc
     1081 gctcccctcc ggagtgagcg cgtacgcctc gtctatgaag aggatcccgc cgagcgcgcg
     1141 ctcgaccgcc gcacggacca gcggcgcggt ctggccgatg taccgcgcca cgaggtcgac
     1201 ccgggacacc tcgaccagat ggcccgacga caggaggtcg agcgactggt agatccgcgc
     1261 gaggagacgc gccaccgtcg tcttcgcggt tccggggttc ccagtgaacg cgaagtggcg
     1321 cgtcggcggc ggcaccttga tcccggcgtc gcggcgtaac cgttcggcct tggcttcggc
     1381 gacgagcagc ccgacctcct gcttcacctg gtgaagcccc acgagtgagt cgagctccgc
     1441 gaacgggtcc tcgtaggcct cccgtccggt gacgtcctcg ggaaggtcct ccacggccac
     1501 ctccggacgc gaggcggtcg cgcgggcacg agcgcgcgct accgcctcgg cagcgaacgc
     1561 cgccgcgaga tgaccgttgc ggaggttgcg gaacgggcgc atgtcgacga gcctccgcgc
     1621 cgcctccgcg gccgtcgcct cgtcgacggc tccaccgaga cgttcgacct tgcgcaggaa
     1681 gacccggtgg aaggtgtcgc ggtcgaagtc gttcgtgcgg acggcacgga aggcccgcgc
     1741 caggatcggg ttcgcggtga agacctccga agcgcggccc gggtcgcaca gcgcgacgac
     1801 gttgaggcct gagcgggcct cgagggcgcg caggagctcg ctcccgaccg cctcggcggc
     1861 ctcgccgccc gcgagaactt cgtccaggcc gtcgatgacg agaagacgga tgccgtcaca
     1921 gcggtcgatc tcctgacgca ccagctcgac cgcgcctagg gcgttcctct cgttgaccac
     1981 cgccgccggc aaccagacgg gaggttgcgc gaagccgcgc accttcagcg cgtccgcgag
     2041 caggtccacc gcgaggccct tgccgctccc ggcgggccct gccacgagca tcctcaccgg
     2101 cccggcggac gcgacggcgt ccgctacggc ctctgcgagc tccggctgac ccaagatctc
     2161 ccccacgggc ggcggcgtcg ccggagcctc gtcggttgtc gcgcgctccg gacgctcgaa
     2221 cttggtgccg agcgcgttcg ggagcgggcg gttcgcaagg agggcgcgga cgttccactc
     2281 gaaggtcgag accggcaccc acaccgtccc cccggggagc atcgccctgc ccggcaggcc
     2341 gttgagagca cccgtcaggc gcatcgccat gtcgagccac gccctgcagg ctgcaagctc
     2401 gccgcggatc gtcgcgcgga ccagccacct ggccgtcgta tccctccacg tctccggcgc
     2461 gaaccccgcg cgcagccggc ggtggtgtcc ttcgatccgg gtgagcaggt cctccgaggc
     2521 gacgactgcg atctccgccg ggacctgctg gatgcccgcc gcgaggacga gctgcgcgat
     2581 cgcaacgtcc tcgtcgtcgc cgtcgccgac cgcagcgcgc agataaggat ggacgtcgag
     2641 gaacccgtcg agggcccaag cgagatagcc gaccggtccg ccgagctccg ggagcgcggc
     2701 gaccgtgcgc tcgtccgccg ccttcgccca ctcgtcggcg agcgcgtcgg cgggagcgtg
     2761 gaggtcgtgc tcgctccccg cgcgttcgtc gtacaaccga gcgagcgccg cggaccgtgc
     2821 cgccacgggc tcgagaccgt cgaagacgcc gacgagcgaa cgcagcacct cgaacgcggt
     2881 cctccggcgc ccgggatcgt cgcccgcggt gaacggcagc agccaccccg gggggcgcca
     2941 ggcaccgctc tgtgtacgca ggtagttcca cgacggtctc cgctcgaaag gctgagccag
     3001 gaacgggtcg aagacccgcc agatcatgtc gccccagtac ccggacctga tcgcgcatcc
     3061 gccgaagagg aaacccaaca tgaggtcgag ggtctccgcg accccgttcg tcggccggcg
     3121 gtaggcctcg cccccttccg ccgcccaacc ggaaaggcgt tcgtcggcca ctgcctgctg
     3181 gaccgcgcgc ccgagggagt cgaggaaccc gtccgggatc ctccacggcc cccacgagta
     3241 gaggtcgagc acgggctcct cgacgaagga gagcgcgagc tcgtcgggaa cgtcgagccg
     3301 gatcacgacc gaacgataga ccggccgata gcgaagcgcc cggggactgg gggcgaccga
     3361 ccggattcga accggcgaca tcctggacca caaccaggtg ctctaaccat gctgagctac
     3421 ggccgccatg aggctccgca agcgtacagc cgcacgactg tgggggctac gcgcctccgc
     3481 ccatcggggc cggcggggtc gggaccatgc gggcgtcccg ccgcgcgtat ccgtacagga
     3541 gagccaaacc gccgaccgct accaatgccg ctacccggaa cgcacccgcg tagccgaacg
     3601 ccgacgccac tgccccgagc gagaccgctc ccaggccgaa cgccaggtcg aagaacgccg
     3661 tgaacgttc
//
LOCUS       DASQDO010000206         6228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_82644, whole genome shotgun sequence.
ACCESSION   DASQDO010000206 DASQDO010000000
VERSION     DASQDO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82644"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /gene="murJ"
                     /locus_tag="VEV43_05920"
     CDS             complement(<1..1446)
                     /gene="murJ"
                     /locus_tag="VEV43_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYP23091.1"
                     /translation="MTEETAAEPKLGRATALMTLGTILSRVTGVARLGLLTWTIGVAE
                     TRLTDTYNLANSAPNVLYELVLGGVVTAVFVPIFVEVMDARTEGERDVSAIVNVCLAG
                     LTILALLGVLAAPAIARFYSTSLSGAEAEAQQHAITTLLRFFLPQIPLYGLYFLGSAF
                     MNVRKRFVLPMWTPIVNNFVLIGVLVAFATLYESVDLRSVTTGQLALLGLGTTASVAP
                     MGLLLLPSLRKLGYRWEWRFDRELWRRVARLSGYAIGFVAANQIGFIAVQKIANESQG
                     AFTAYTAAFTFFLLPVGLFVWSITTALAPSLSRAALEPGRAGMADVLSIALRSTLFLI
                     VPCAAGFLVLARPVVEVMLGHGVVTEASTDLVVDILSFLVLGLVQFSLFQVLMRASYA
                     MQDARSPFVVNAVVIAVNVALNLAMFRWIGPEGLAAGHALAYTIAVLLQGRVVAREVP
                     GLDLRRVATSTAKITVAAGAMAAVVAGGYRLM"
     gene            complement(1443..2078)
                     /locus_tag="VEV43_05925"
     CDS             complement(1443..2078)
                     /locus_tag="VEV43_05925"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HYP23092.1"
                     /translation="MSERQTWPPGLRGDLARLFSSQGTHEGKPSLTGALVRAVTRPGP
                     LAILLYRLGHRLWERGFHTLAEVLWRLNFFLSGADIHPGAKIGGGLRLTHTAGLVVGK
                     GAVIGSDVTLLHGVTLGGSSRGFFDPEFTDGFPTIGDGSKIAAGAKLLGPITIGKGCF
                     IGANAVVAKDLPDGTIYTPGRKVSELSKRIDELEAELADLRRAVAESEAAR"
     gene            complement(2207..2386)
                     /locus_tag="VEV43_05930"
                     /pseudo
     CDS             complement(2207..2386)
                     /locus_tag="VEV43_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
     gene            2554..3006
                     /locus_tag="VEV43_05935"
     CDS             2554..3006
                     /locus_tag="VEV43_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23093.1"
                     /translation="MRLRRAVRLLVFSALVGMITGTVYSATAHTECTPSGFDPVVHEC
                     DIHHNDWGAGGGYDSLFGFGGGDTLRAASGRDRNRGATGTDFLKDEEGSSDQDEFCDG
                     GDFDYIYMGDGDGQDVLYYTGGGYTMSSEDFEEGDDVTNDYLSSGCPV"
     gene            3062..3595
                     /locus_tag="VEV43_05940"
     CDS             3062..3595
                     /locus_tag="VEV43_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HYP23094.1"
                     /translation="MHGSRAVRPVAVSHKPPDVLLQLYERAAPGAYRLALLLSGDRDE
                     ADDVVQEAFVRIMRRSSGSIRPESLDAYLRRTVVNLTRSRWRRIRSQRRYLETHRPRP
                     EEPTTQLDVESRHEMWTTLRTLPVRQRAVLFLRYYEDMSETQVAEVLGCSTNAVKCLA
                     ARALTSLRRLPRGVMDE"
     gene            3931..4596
                     /locus_tag="VEV43_05945"
     CDS             3931..4596
                     /locus_tag="VEV43_05945"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23095.1"
                     /translation="MASVPGSGGVEEKEALCLSMGFGSSRRAEATCTVGLQEPFVGND
                     AVYAVPSSAPGWNRAFFGIVDKKVERVELRLDDGTSYAAEIHNGGPELRAPFNFFVGF
                     VPEWRDVTIVAVDRSGATAETDHLPAPPRLTVTKTGDGAGTVTGTRTEEIGCSDCEPH
                     RWIDCGEECLALINDASVTLEAHPDEGSVFVRWDGACSGVLPCVLQVDSDKTVDAVFQ
                     KVP"
     gene            complement(4635..5060)
                     /locus_tag="VEV43_05950"
     CDS             complement(4635..5060)
                     /locus_tag="VEV43_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HYP23096.1"
                     /translation="MRVVLGSDMKTPLTDAVARDLEERGHDVALVGPPGGEEIEWAEV
                     GHRVGAAVAGSEADTGVLFCWTGTGASIAANKVPGARAALCTDASTAEGARKYNDANV
                     LVMGLRLTSPEVAKEMLDAWFTAEPDPSEAANVARLEPS"
     gene            complement(5071..5763)
                     /locus_tag="VEV43_05955"
     CDS             complement(5071..5763)
                     /locus_tag="VEV43_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYP23097.1"
                     /translation="MELRQNGLEQAAPDSAALAAEVDRLQMTEKELRLAQEAVVERLS
                     IAAEFRDDETARHVQRMSRYTALLASRAGANTERAEQVRTAAVMHDVGKIGIPDSILL
                     KPGKLTPEERRIMEQHSEIGYRILSGSRSELLTLAAQIAWTHHERVDGNGYPRRLAGD
                     DIPLEGRMAAIADVFDALTSNKVYKKASPLPQAVEIMREGRGTHFDADLLDMFLDSID
                     EVVRIKERFADR"
     gene            complement(5880..>6228)
                     /locus_tag="VEV43_05960"
     CDS             complement(5880..>6228)
                     /locus_tag="VEV43_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23098.1"
                     /translation="ADVDEAVGPLRDDPSVGEPARAVAESAARIRALAPGAGEEAARE
                     IALSVARTVACAELCKQGAWATRRGSGRTAAIAARLAARGLGPGVVVDEAGLELAGWG
                     AEEAPAQPSDVST"
BASE COUNT         1011 a         2095 c         2179 g          943 t
ORIGIN      
        1 catgaggcgg tagccgcccg cgacgaccgc ggccatggcc ccggctgcaa ccgtgatctt
       61 cgcagtgctc gtggcgacgc ggcgcaggtc gagcccgggc acctcgcgcg cgacgacccg
      121 gccctgcagc agcaccgcga tcgtgtacgc aagcgcgtgc ccggccgcca gtccctcggg
      181 cccgatccat cggaacatcg ccaggttcag cgcgacgttc acggcgatca ccaccgcgtt
      241 gacgacgaag ggcgagcgcg catcctgcat cgcgtaggac gcgcgcatca gcacctggaa
      301 gagcgagaac tggacgaggc cgagcacgag gaacgacagg atgtcgacga ctaggtcggt
      361 cgacgcctcg gtgacgacgc cgtggccgag catcacctcg acgactgggc gggcgaggac
      421 gaggaacccc gccgcgcacg ggacgatcaa gaacaacgtc gaccgcagcg cgatcgagag
      481 cacgtccgcc atccccgctc gccccggttc gagagcggcg cggctcagag acggcgcgag
      541 ggccgtcgtg atcgaccaca cgaacaggcc gacggggagc aggaagaacg tgaacgcagc
      601 ggtgtaggcg gtgaacgctc cctggctctc gttggcgatc ttctggaccg cgatgaaccc
      661 gatctgattg gccgcgacga acccgatcgc gtagccggag agccgcgcga cgcggcgcca
      721 cagctcgcga tcgaaccgcc actcccagcg gtagccgagc ttgcgcagcg acgggaggag
      781 caacaggccc atcggcgcga cgctcgcggt cgtgccgagg ccgagcagcg ccagctgtcc
      841 cgtcgtgacc gaccgcaggt cgacgctctc gtagagggtc gcgaacgcga cgagcacgcc
      901 gatcagcacg aagttgttga cgatcggcgt ccacatcggg agcacgaacc tcttgcggac
      961 gttcatgaac gcgctgccga ggaagtagag cccgtagaga gggatctgcg ggaggaagaa
     1021 ccggagcagg gtcgtgatcg cgtgctgctg cgcctcggcc tcggcccccg acaggctcgt
     1081 cgagtagaag cgagcgatcg ccggcgccgc gagcacgccc agcagggcga ggatcgtcag
     1141 cccggcgagg cacacgttga cgatcgcgct gacgtcgcgc tccccttctg tccgcgcgtc
     1201 catgacctcg acgaagatcg gcacgaacac cgccgtgacg acgccgccga ggacgagctc
     1261 gtagaggacg ttgggggccg agttcgccag gttgtacgtg tcggtcagac gcgtctctgc
     1321 gacgccgatc gtccacgtca acaggccgag gcgcgccacg ccggtgacgc gcgacaggat
     1381 cgtaccgagc gtcatgagcg cggtcgcgcg ccccagcttc ggctccgctg cggtctcttc
     1441 cgtcacctcg ccgcttcgga ttcggccacg gcccgccgca ggtcggcgag ctccgcctcg
     1501 agctcgtcga tgcgcttcga cagctccgag accttgcggc ccggcgtgta gatcgtgccg
     1561 tcggggaggt ccttagcgac gaccgcgttc gcgccgatga agcagccctt gccgatcgtg
     1621 atcgggccga ggagcttcgc cccggccgcg atcttcgagc cgtcgccgat cgtcgggaac
     1681 ccgtcggtga actcgggatc gaagaagccc cgcgacgacc ctccgagcgt gacgccgtgc
     1741 aacagcgtga cgtccgagcc gatcacggcc cccttgccga ccacgagccc ggcggtgtgc
     1801 gtgagccgga ggccgccgcc gatcttcgcc ccggggtgga tgtccgcgcc gctgaggaag
     1861 aagttgagcc gccataaaac ctcggcaagc gtgtggaagc cgcgctccca caagcggtgg
     1921 cccaggcggt agagcaggat cgcgaggggg ccgggccgcg tgacggcgcg cacgagcgcc
     1981 ccggtcagcg acggtttgcc ctcatgggtg ccctgcgagg agaacaggcg cgcgaggtcg
     2041 ccgcgcaggc ccgggggcca ggtctggcgc tcgctcacga ggtctcgctc agcgtgctct
     2101 tgatgtcgcg cagctcctcg gcggtgatct cgccgcgggc gtagcgctcc tcggcgatcg
     2161 cgatcgcggg gttgcggcgc ccgacgagcg agatgccgtc gggttcttcg gggagcacga
     2221 cccacaggac gaggtagagg aggccgcccg cgccggcgag gaggacggag aggacccagg
     2281 cgacgcgcac gaggctgggc tcgaggccga agtagtgcgc gaccccggcg gcgacgccgg
     2341 cgatcttgcg gtcggacgcg gaacgggcga gcctcttgtc ggtcacgggc ccaatggtgc
     2401 cacgggggcc ggaaccgggg cgtatcggcc gacgaccgca ggccccccac atcgccggtc
     2461 gacatccgtc ggacccgcgg cgtccttact atcttgggtt ctcaccgagt taagcttcta
     2521 attcaatggc cggagagcca aacggaggtg tgcatgcggc taaggagagc ggttcgactc
     2581 ttggtcttca gtgcactggt ggggatgatc acaggcacgg tgtactcggc cacggcccac
     2641 acggaatgca ccccgtccgg gttcgaccca gtcgtccacg agtgcgacat ccaccacaac
     2701 gactgggggg ccggaggggg ctatgacagc ttgttcggct tcggaggcgg cgacaccctt
     2761 agagctgcct cgggacgcga tcgcaaccga ggggcgaccg ggacagattt cctgaaggac
     2821 gaggagggaa gcagcgatca ggacgagttc tgcgacggcg gcgacttcga ctacatctat
     2881 atgggcgacg gcgacggcca ggacgtcttg tattacacgg gtggcggcta cacgatgagt
     2941 agcgaggact tcgaagaggg cgacgacgtg accaacgact acctgagctc agggtgtccg
     3001 gtttgacaat ggccgccgac ctcttttggt gccttcggac gtctaggggt atgtgaagga
     3061 aatgcacgga tcgcgcgcgg tacgcccggt cgccgtgtcg cacaagcccc cggacgtgct
     3121 cctccagctc tacgagaggg ccgcccctgg cgcttatcgc ctcgcgctgc tgctcagcgg
     3181 cgaccgcgac gaggccgacg acgtcgtcca ggaggccttc gtgcggatca tgcggcgatc
     3241 gtcggggtcg atacggcccg agtccctgga cgcttacctt cgacgcacgg tcgtaaacct
     3301 cacgcgtagc cggtggcgcc ggattcgatc acagcgacgc tatctcgaga cgcatcggcc
     3361 gcgtcccgag gagccgacga cgcaactaga cgtggaatcg cggcacgaga tgtggacgac
     3421 gctgcgcacc ctgcccgtcc gccaacgcgc cgtgctcttt ctccgctact acgaggacat
     3481 gtccgagaca caggttgccg aagtcctcgg atgttcgacc aacgcggtga aatgcctcgc
     3541 agcccgcgcg ctgaccagcc tccggcggct tccgagagga gtgatggacg aatgacgccc
     3601 gatgagaaag agcttcgtcg cctgctcaag gcagagctgg atcaaagcca tccgagcccg
     3661 ctcggagcgc gggctcgacg aagaatcgtc acgggccgcg caattttcgt agccgccacg
     3721 agtctcgcca tggccgctac ggcctacgcc gggtttgcga tcactcaggt agcggcggac
     3781 ctgaccggat cgcccgatca gcagtctgtt tcgggggcgc agggagaaga aaagttcgag
     3841 ccccgcgagc ctcgcccgat cacaccgaag gtcgaggtcg ccacaggtcg tcagcaggga
     3901 tccgcttggt cggtcttctc gtaccgcgcg atggcgtcgg ttcccgggag cggcggcgtc
     3961 gaggagaaag aggctctctg cctttcgatg gggttcgggt cgtcgaggcg cgcggaggcg
     4021 acttgcacgg tgggcctgca ggagcccttc gtcggcaacg atgcggtgta tgcggtgccg
     4081 agctcggcgc cggggtggaa ccgcgcgttc ttcgggatcg tcgacaagaa ggtggagcgc
     4141 gtcgagctac gtctcgatga cgggacctcg tacgccgcgg agattcacaa cggtgggccc
     4201 gagttgcgcg ctcctttcaa cttcttcgtc gggttcgttc cggagtggcg agacgtcacc
     4261 atcgtcgccg ttgaccggtc cggcgcaact gccgagacgg accaccttcc agcacccccg
     4321 cggctgacgg tgacgaagac gggcgacggc gccgggacgg tgacgggaac ccggacagag
     4381 gaaatcggct gcagcgattg tgagccgcac cgttggatcg actgcggaga ggaatgtctg
     4441 gccctgataa acgatgcgag cgtcacgctg gaggcccacc cggacgaggg ttcggtattc
     4501 gtacgatggg acggagcatg ctccggagtt ctgccgtgcg tgctgcaggt cgactccgac
     4561 aagactgtcg acgcggtctt tcagaaggtt ccgtagagcg atacgggcgg ggacgccttg
     4621 ctttaccgaa cccgctagct cggttcgagc ctcgccacgt tggcggcttc cgacgggtcc
     4681 ggctccgccg tgaaccacgc gtccagcatc tccttcgcca cctcgggcga ggtcaggcgc
     4741 aggcccatca ccaggacgtt cgcgtcgttg tacttgcggg ccccctcggc cgtggacgcg
     4801 tcggtgcaca gcgcggccct tgcgcccggg accttgttcg ccgcgatcga cgcacccgtg
     4861 ccggtccagc agaacagcac gccggtgtcc gcctcgctac cggcgaccgc ggctccaacc
     4921 ctgtgaccga cctcggccca ctcgatctcc tcgccgccgg gaggcccgac gagcgcgacg
     4981 tcgtggccgc gctcttcgag atcgcgcgcg accgcgtcgg tgaggggggt cttcatgtcc
     5041 gagcccagga cgactcgcat gagcacgatg ttagcggtcg gcgaaccgct ccttgatgcg
     5101 gacgacctcg tcgatcgagt ccaggaacat gtcgagcagg tccgcgtcga agtgcgtccc
     5161 gcggccctca cgcatgatct cgaccgcctg cggaagcggc gacgccttct tgtagacctt
     5221 gttcgaggtc agcgcgtcga atacgtcggc gatcgccgcc atccggccct cgagcgggat
     5281 gtcgtcgccc gccagccggc gcgggtagcc gttgccgtcg acacgctcgt gatgcgtcca
     5341 cgcgatctgg gcggcgagcg tcagcagctc ggaacgggag ccggacagga tccggtagcc
     5401 gatctcgctg tgctgctcca tgatccggcg ctcctccggc gtgagcttgc cgggcttcag
     5461 aaggatgctg tcggggatgc cgatcttgcc gacgtcgtgc atgaccgcgg cggtccgcac
     5521 ctgctcggcg cgctcggtgt tcgccccggc gcggctggcc agcaacgcgg tgtagcggct
     5581 catcctctgg acgtggcgag cggtctcgtc gtcacggaac tccgcagcga tcgagaggcg
     5641 ctcgacgacg gcctcctgcg cgagccgcag ctccttctcc gtcatctgga gacggtcgac
     5701 ctcggccgcg agcgcggcgc tgtcgggagc agcctgctct agaccgttct ggcggagctc
     5761 catctcggaa ctgttgatga cgcctctcct ctacgtcgac ggcgcggttg caccggtggc
     5821 ctcttccttc catcgggacg agcgggccgg gaattgaggt cggggggcgg aaagtttgct
     5881 caggtcgaga cgtcgctcgg ctgcgcggga gcctcctcgg ccccccagcc ggcgagctcg
     5941 agccccgctt cgtcgacgac gacgccgggc ccgaggcccc gggccgcgag gcgggcggcg
     6001 atcgcggccg ttcgtccgct cccccgccgg gtcgcccagg ccccctgctt gcacagctcg
     6061 gcacaggcga cggtgcgggc gacggacagc gcgatctctc gcgccgcctc ctccccggcc
     6121 cccggagcca gcgctcggat gcgggccgcg ctctcggcga cggcccgggc aggctctccc
     6181 accgaggggt cgtcgcgcag cggcccgacc gcctcgtcga cgtccgcg
//
LOCUS       DASQDO010000207         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_878032, whole genome shotgun sequence.
ACCESSION   DASQDO010000207 DASQDO010000000
VERSION     DASQDO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878032"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1705
                     /locus_tag="VEV43_05965"
     CDS             50..1705
                     /locus_tag="VEV43_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYP23099.1"
                     /translation="MADDMISRWRERYEQARLRDDVDFDTLSSVELEPLYGPPDGSVP
                     ETIGVPGEYPFTRGIYPSGYRGRLWTMRQFAGFGTADETNARFKFLTESGQTGLSVAF
                     DMPTLMGRDSDDPHSMGEVGRCGVAVDSLKDMEVLFEGIDLEGITTSMTISGPAPMVF
                     AMYIAAAMKQGATVRNLGGTCQTDILKEYIAQKEWLFPPEPHLRLTADMMEYCLSDVP
                     KYYPLSISGYHIREAGSTAAQELAFTIASGFAHVELGTRRGMAVDDVAPRLSFFFNSH
                     IDFFEEIAKFRAARRIWARWMKDRYGATTPRAQMLKFHTQTAGCSLTAQQPYNNVVRT
                     ATEALAAVLGGTQSLHTNSLDEVLALPTDEAVEIALRTQQVIAYETGVTNVADPLGGS
                     WFVEALTDKIEDAAEAYFARIDEMGEGSILDGMLRGIEQGYFQKEMADAAFKYQQLLE
                     QERKVIVGVNRFTKTTQEMPEVLLIGLETEERQKKAVAEVRANRDQQKADDAIAALKR
                     MADEPTVNSIPVLVDAALAYVTLGEMVEALKDVWGVYVEPPMF"
     gene            complement(1702..2127)
                     /locus_tag="VEV43_05970"
     CDS             complement(1702..2127)
                     /locus_tag="VEV43_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23100.1"
                     /translation="MRRVVVLVCLAAVTVLPQPGRAACAAPSVRAMPAERRAGQLVLV
                     TGEDWASGCDDVVGDECTGACAGCPDVVEPEKIEQVVVELRREGAEPARLYTADVSVQ
                     SSFAEEVRLPRDLEPGRYRIVAITEQDGYRARARLVIVD"
     gene            complement(2132..2890)
                     /locus_tag="VEV43_05975"
     CDS             complement(2132..2890)
                     /locus_tag="VEV43_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYP23101.1"
                     /translation="MLEVERRGAVDLLWLDRPPVNAADLESLAALADAVYQVEASSDR
                     PLVLSGRGPAFSAGADLYGVLDGDESYAARFVAALVRAFTTLFSFPRPLVGAVNGHAI
                     AGGAILACCCDHVVCADGRLKVGVSELAVGVPYPLAALEVVRHAVAPERFEEVVFLAG
                     TYSPAEARTIGFVDEVVPAESVLDRAVSVAETLGRVPAATYELIKKAVRAPALERIAQ
                     QGPAHDEAATRGWQSTEVRESIRAFLDRTVRKDG"
     gene            3057..>3878
                     /locus_tag="VEV43_05980"
     CDS             3057..>3878
                     /locus_tag="VEV43_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYP23102.1"
                     /translation="MRRLRARTSLLAEFAVVSFVALVVLGVVLAQTFLRTTKQEERDQ
                     ALQVASLTARADIRPRIPAEDVATTLGHAERDLLDDKLDATADRSGVEGVTIWDRGAE
                     VVYSTTGAPSTRVEDSPHVKKALAGQTETVVGHETALGTSSPKELVQVYLPLKTDNGV
                     TAGAVEVDFVYDHLVAGHKEDQKTILGVLAGSLGVIYLLMLGTVARSSRTHHRQARET
                     EHQAVHDALTGLHNRAYFRERVEQAISEGKVSGERACLMLIDLDRFKEVNDTLGHH"
BASE COUNT          647 a         1412 c         1296 g          523 t
ORIGIN      
        1 tcagtggacg ctaggccggg ccccacgccg ccccctgtag aatccgtcca tggctgatga
       61 catgatctcg cgctggcgcg agcggtacga gcaggcgcgg ctccgcgacg acgtcgactt
      121 cgacacgctg tcgagcgtcg agctcgagcc cctctacggc ccccccgacg gaagcgtccc
      181 cgagaccatc ggcgtccccg gtgagtatcc gttcacccgc ggcatctacc cgagcgggta
      241 ccgcggccgg ctgtggacga tgcgccagtt cgccgggttc ggcaccgcgg acgagaccaa
      301 cgcacgcttc aagttcctga ccgagtcggg ccagaccggg ctcagcgtcg cgttcgacat
      361 gccgacgctc atgggccggg actcggacga cccgcactcg atgggcgagg tcgggcgctg
      421 cggcgtcgcg gtggactccc tgaaggacat ggaggtcctc ttcgagggca tcgatctcga
      481 gggcatcacg acctcgatga cgatctcggg cccggccccc atggtcttcg cgatgtacat
      541 cgccgcggcg atgaagcagg gcgcgaccgt ccgcaacctc ggcggcacgt gccagaccga
      601 catcctcaag gagtacatcg cccagaagga gtggctgttc ccgcccgagc cccacctgcg
      661 gctgacggcg gacatgatgg agtactgcct gagcgacgtg ccgaagtact acccgctgtc
      721 gatctccggc taccacatca gggaggccgg gagcacggcg gcgcaggagc tcgcgttcac
      781 gatcgccagc ggcttcgcgc acgtcgagct cggcacccgc aggggcatgg ccgtcgacga
      841 cgtagccccg cgcctctcgt tcttcttcaa ctcccacatc gacttcttcg aggagatcgc
      901 caagttccgg gccgctcgcc ggatctgggc ccgctggatg aaggaccgct acggcgcgac
      961 gacgccgagg gcccagatgc tgaagttcca cacgcagacg gcgggctgct cgctcacggc
     1021 acagcagccg tacaacaacg tcgtacgcac cgcgaccgag gcactggccg cggtcctcgg
     1081 cgggacgcag agcctccaca cgaactcgct cgacgaggtg ctcgcgctcc cgaccgacga
     1141 agccgtcgag atcgcgctcc ggacccagca ggtcatcgcc tacgagaccg gcgtgacgaa
     1201 cgtcgccgac ccgctcggcg gctcgtggtt cgtcgaggcg ctgaccgaca agatcgaaga
     1261 cgcggccgag gcctacttcg cccgcatcga cgagatgggc gagggctcga tcctcgacgg
     1321 catgctccgc ggcatcgagc agggctactt ccagaaggag atggccgacg ccgcgttcaa
     1381 gtaccagcag ctcctcgagc aggagcgcaa ggtcatcgtc ggcgtcaacc gcttcacgaa
     1441 gacgacgcag gagatgcccg aggtcctgtt gatcggcctc gagaccgagg agcgtcagaa
     1501 gaaggccgtc gccgaggtcc gggcgaaccg cgaccagcag aaggccgacg acgcgatcgc
     1561 ggccctgaag cggatggcgg acgagccgac ggtgaactcg atcccggtgc tggtcgacgc
     1621 ggcgctggcg tacgtgacgc tgggcgagat ggtcgaggcg ctgaaggacg tgtggggggt
     1681 ctacgtagag cccccgatgt tctagtcgac gatcaccagg cgggcccggg cccgatagcc
     1741 gtcctgctcg gtgatggcca cgatcctgta acgacccggc tcgagatcgc gcggtagccg
     1801 cacctcttcg gcgaacgacg actgaaccga gacgtcggcg gtgtagagcc ttgccggctc
     1861 ggccccctcg cgtctcagct ccacgaccac ctgctcgatc ttctcgggct cgaccacgtc
     1921 gggacaaccg gcacatgccc ccgtgcactc gtcgccgacg acgtcgtcgc acccgctcgc
     1981 ccagtcctct ccggtgacga ggacgagctg cccggcccgg cgctccgccg gcatcgcccg
     2041 tacggagggc gctgcgcacg ccgcccttcc cggctgcggc aggaccgtca cggccgcgag
     2101 gcaaacgagc acgaccaccc gtcgcacgcc gctatccgtc cttccgcacg gtccggtcca
     2161 ggaacgctcg gatcgactcc ctgacctcgg tcgattgcca gcccctcgtc gcggcctcgt
     2221 cgtgggccgg accctgctgc gcgatccgct ccagcgccgg cgcgcgcacc gccttcttga
     2281 tcagctcgta agtcgccgcc gggacccgcc cgagcgtctc ggcgaccgac acggcccggt
     2341 ccaggaccga ctccgcgggc acgacctcgt cgacgaaccc gatcgtgcgc gcctcggccg
     2401 gcgagtaggt gcccgccagg aagacgacct cctcgaacct ctccggtgcc accgcgtgcc
     2461 ggacgacctc gagcgccgcc agcggatagg ggactccgac ggcgagctcg gagacgccga
     2521 ccttcagccg cccgtccgcg cacaccacgt ggtcgcagca gcaggcgagg atcgccccgc
     2581 ccgcgatcgc gtgcccgttc accgcaccga ccaggggccg ggggaacgag aacagcgtcg
     2641 tgaacgcccg gaccagcgcg gccacgaacc gagcggcgta cgactcgtct ccgtccagga
     2701 cgccgtagag gtcggccccc gcggagaagg caggaccgcg gccgctcagc accagcggcc
     2761 ggtccgagga cgcctcgacc tggtagacgg cgtcggcgag ggccgcgagc gattcgaggt
     2821 ccgccgcgtt caccgggggc cggtccagcc acagcaggtc gacggcgccg cgccgctcca
     2881 cctcgagcac gttcgggagg ctatcccagc gcgccggacg gccctacgcg gtgagcgctt
     2941 tttccccctt tcggggggtc gcccctgctc aaggcccccc tcggcgctgc cgatgcccac
     3001 ggggaacggt cggagagccg gaaggctccg gctgcctggg agggacggaa taacggatgc
     3061 gtaggctaag agcgaggacc agcctgctgg cggagttcgc cgtcgtcagc ttcgtcgcgc
     3121 tcgtcgtgtt gggggtcgtc ctcgcacaga ccttcctgcg cactacgaag caggaagagc
     3181 gggatcaggc cctccaggtc gcgagcctca cggcccgcgc ggacatccgc ccccggatcc
     3241 ccgccgaaga cgtcgccacg acgctcggcc acgcggagcg cgatctcctg gacgacaagc
     3301 tcgacgcgac cgcggacagg agcggcgtcg agggcgtcac gatctgggac aggggggccg
     3361 aggtcgtcta ttcgacgacg ggagcgccct ccacccgggt cgaggactcg ccgcacgtca
     3421 agaaggcgct cgccggccag accgagaccg tcgtcggcca cgagaccgcg ctcggcacgt
     3481 cgagccccaa ggagctcgtc caggtctacc tgccgctcaa gaccgacaac ggggtcaccg
     3541 cgggtgcggt cgaggtcgac ttcgtctacg accacctcgt cgcgggccac aaggaggatc
     3601 agaagacgat cctcggcgtg ctcgccgggt cgctcggtgt gatctacctc ctgatgctcg
     3661 ggaccgtcgc acgctcgtcg cgcacgcacc accgccaggc tcgcgagacc gagcaccagg
     3721 ccgtgcacga cgcgctcacc gggctccaca accgcgccta cttccgggag cgcgtcgagc
     3781 aggcgatctc cgagggcaag gtcagcggcg agagggcatg tctcatgctc atcgacctcg
     3841 accgcttcaa ggaggtcaac gacacgctcg gccatcac
//
LOCUS       DASQDO010000208         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_21292, whole genome shotgun sequence.
ACCESSION   DASQDO010000208 DASQDO010000000
VERSION     DASQDO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21292"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1893)
                     /locus_tag="VEV43_05985"
     CDS             complement(247..1893)
                     /locus_tag="VEV43_05985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYP23103.1"
                     /translation="MRTHKAGAGRAAEADRGGQASARSESPEIVSLAERMGYLQVLRA
                     GFVVVVLASAIFASRFVGASLRDLVVVSAGYFVLTGVTEALRRLGKARGLYVVAGMLL
                     VDGVYLAGTMYATGFAQSPLRFLLYLHLIAVTLLASYRTGLKIALWHSLLFFVVFHGQ
                     LAGILAPQGVDVTGDIGALTYRPSVFNVMAFWLVALGTAVFSSVNEKELRRRKIDLEA
                     LNEMGVEFENVSDPVTVAATFLDRVCDAFDFKRGVVLASPAGDVSVVAARGAGGDDDP
                     SLGLDDVVAKTWRKRETSLVRKFDAEANPRLARLLPGARNVLIVPLFADAAPLGVLAV
                     EHGGRSSKIERRVLTTVGQFSSHAALAMRNAWLLEEIRRMADTDGLTGIANRRFFESA
                     LEKEISRAKRSGEQLTLVMLDIDHFKLLNDTHGHQVGDQILQEVGSALDKTCRDFDVP
                     ARYGGEEFAVLLPGCTTQESFSAAGRLRKMISEMEGAVPVTASAGVATFPLHATDAAG
                     LVKAADEALYESKRMGRDRVTRSRRKGVAVVEQKVAEPVV"
     gene            complement(1890..2768)
                     /locus_tag="VEV43_05990"
     CDS             complement(1890..2768)
                     /locus_tag="VEV43_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23104.1"
                     /translation="MNRGGSVTTDSRRLFAALVVSFALLQAFTFLPTAVGAPGDSCDK
                     PGNGNDKKCETPAPTPPPPTPTPSASPSASPSPSLPPSSSPKPSEPPVTTPPATDPAT
                     PPPSMEPTPSATPPPATEPPVITPPPIATEPPPTAPPGPTPIGTQPPVPVGPQPPGVD
                     TDSPEPLGPTETDDPRGSAGFPSLRDGGGPGGEILEALSDLDALAVSVADPKPGSVTD
                     EEPAGHAFGLRVPLGSSIGNLAATAGKAVQEFAFPLTLAIAVLLFLLFQGELDRRDPK
                     LAYAPVDSTKDMVSFE"
     gene            complement(2869..4458)
                     /locus_tag="VEV43_05995"
     CDS             complement(2869..4458)
                     /locus_tag="VEV43_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYP23105.1"
                     /translation="MRGRGSSTGRSAEIVSLAERMGYLQGLRAGFVAVAMASALLFPG
                     FVGASLRDVAVVSFAYFVLTGVIEAVRRIGGGRHLYVVAGMLLADGVYLAWIMYLTGF
                     VQSPLRFMLYLHLIAVTLLASYRTGLKIALWHSLLFFVVFHAQLAGLLDPRGPSLHMP
                     GMEGLVEYRPSIFNVTAFWVVALGTALFSFLNERELRRRKNDLEALNEMGVEFESVSD
                     PRNVAESCLGRVCDALGFRRGLVVAGPPGALTVLATKGAEGVGTPVPEDCGLLLEVAQ
                     GRETTLVTDLDPGTDGVIAELLPGARDLLVTPLIADGNQLGVLVVEQRRRGHRLERRV
                     LSTIGQFSSHAALALRNAWLLEEVRKMADTDGLTGLANRRSFEDALDGEVARALRTGE
                     QLTLVLLDIDYFKDLNDRYGHQVGDGVLRATGAALRDASREFDTAARYGGEEFVVLLP
                     GCSSDQAIAAAERLRAAVAAVDEDVAITASAGVATLPLNASDAAGLVRAADQALYDSK
                     RRGRDVTTRSSAVGDFVPSAL"
     gene            complement(4455..>5138)
                     /locus_tag="VEV43_06000"
     CDS             complement(4455..>5138)
                     /locus_tag="VEV43_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23106.1"
                     /translation="EPTPTPIATVEPTEPPPPPPPPTPEPTETPTPEPTPTPTPDPTP
                     TPEVVPSFRPPVWPPELPPAPVEIGSTPPPEQTLPPEPPLPTPDFTTPPPGPGPLAPL
                     ATPNPRPDFGGEDAGEGTGERKDDPTGPGNGTGEDEPPRRLIDLTGDGSATSGGVGTP
                     GGTRNGFTASIADLARTAGEAVKTFAFPLSLTILVVIFLLIQGEIDRRDPKLAFAPVD
                     SSKDMVYFE"
BASE COUNT          777 a         1800 c         1864 g          697 t
ORIGIN      
        1 gcgggatcgt gaggtcgagg tcgcgcaacg cctccacgac gccggccccg gtctcgtaga
       61 ccttgcgcac gtgcgacgcg acgaggatcg cgccggtcgc gacgtcggga gtcgtggggg
      121 cctcggcttt caccgggggc aggatagctg cgcgtctccg ctacgaaccg accgaatccg
      181 gcataacgac tacgcaatcg acgcgccttc gtcccaaggc cgtcaccccc cgcggggcaa
      241 cctcggctag acgaccggct cggctacctt ctgctcgacc acggcaacgc ccttgcgccg
      301 cgagcgcgtg accctgtcgc ggcccatgcg cttgctctcg tacagcgctt cgtcggccgc
      361 cttgacgagc ccggctgcgt cggtcgcgtg cagcgggaac gtcgccacgc cggcgctggc
      421 cgtcaccggc accgcgccct ccatctcgga gatcatcttg cggaggcggc ccgcggcgct
      481 gaacgactcc tgcgtcgtgc agcccgggag cagcaccgcg aactcctcac cgccgtaacg
      541 tgcgggcacg tcgaagtccc ggcaggtctt gtcgagggcg cttccgacct cctgcaggat
      601 ctggtcgccg acctggtggc cgtgcgtgtc gttcagcagc ttgaagtggt cgatgtcgag
      661 catcacgagc gtcagctgct cgccgctgcg cttcgcccgg gagatctcct tctcgagagc
      721 gctctcgaag aaccggcggt tcgcaatccc ggtcaggccg tcggtgtcgg ccatgcggcg
      781 gatctcttcg agcagccacg cgttgcgcat cgcgagggcc gcgtgcgacg agaactggcc
      841 gacggtcgtg aggacccggc gctcgatctt gctggagcgt ccaccgtgct cgaccgcgag
      901 caccccgagc ggagcggcgt ccgcgaacag cgggacgatg aggacgttgc gggcacccgg
      961 cagcagtcgc gccaggcgcg ggttcgcctc cgcgtcgaac ttgcggacga gcgaggtctc
     1021 acgcttgcgc cacgtcttcg cgacgacgtc gtcgaggccc agcgagggat cgtcgtcacc
     1081 cccggccccc ctggccgcaa ccacggacac gtcaccggcg gggctcgcca gcactacgcc
     1141 gcgcttgaag tcgaacgcgt cgcacacacg gtcgaggaac gtcgccgcga ccgtcaccgg
     1201 gtcgctcacg ttctcgaact cgacacccat ctcgttcagc gcctcgaggt cgatcttgcg
     1261 gcggcggagc tccttctcgt tcaccgagga gaagaccgcc gtgccgagtg cgacgagcca
     1321 gaaagccatg acgttgaaca ccgaggggcg gtaggtgagg gcacctatgt cgccggtgac
     1381 gtcgacgccc tgcggcgcca ggatcccggc gagctgtccg tggaagacca cgaagaacaa
     1441 cagcgagtgc cacagcgcga tcttcaaccc ggtccggtac gaggcgagga gcgtcaccgc
     1501 gatcaggtgc aggtacagca ggaagcgaag ggggctctgc gcgaagccgg tcgcgtacat
     1561 cgtccctgcg aggtagacac cgtcgacgag gagcatcccg gccacgacgt acaggccgcg
     1621 cgccttcccg agccgccgga gcgcctcggt tacgccggtg aggacgaagt aacccgccga
     1681 gaccacgacg aggtcgcgca gcgacgcccc gacgaacctc gaggcgaaga tcgccgacgc
     1741 gagcacgacg acgacgaagc cggcgcgaag cacctgcagg taccccatcc gctcggcgag
     1801 agagacgatc tccggggact ccgaacgcgc gctcgcctgc cccccacggt ccgcctcggc
     1861 ggcccgccca gccccagcct tgtgcgtcct cattcgaagc tcaccatgtc ctttgtcgag
     1921 tcgacgggcg cgtaggcgag cttcggatcc ctccgatcca gctcaccttg gaacaacagg
     1981 aacagcagca ctgcgattgc cagagtcaac gggaacgcga actcctggac ggccttgccg
     2041 gcggtagccg cgaggttccc tatcgacgag ccgagcggga cgcgcagccc gaaggcgtga
     2101 ccggccggct cttcgtccgt gacggagccg ggcttcgggt cggcgacgct cacggcgagc
     2161 gcgtccaggt ccgacagcgc ctcgaggatc tcgccaccag gtccgccgcc gtcacggagc
     2221 gacgggaagc cggcgcttcc gcgagggtcg tcggtctcgg tcggcccgag cggctcggga
     2281 gaatccgtgt ccacgcccgg cggctgcggg cccaccggca cagggggctg ggtccctatc
     2341 ggggtcgggc ccggcggcgc ggtcggaggc ggctccgtcg cgatcggagg cggcgtgatg
     2401 accggcggct cggtcgcggg cggaggcgta gcgctcgggg tcggctccat cgacggcggg
     2461 ggcgtcgccg ggtcggtcgc cggcggcgtg gtgaccggcg gctcggacgg cttcggcgac
     2521 gagctgggcg gcagagacgg cgacgggctg gcagacggag aagccgacgg ggtcggggtg
     2581 gggggaggag gcgtcggcgc cggcgtctcg cacttcttgt cgttcccgtt gcccggcttg
     2641 tcgcaggagt ctccgggggc ccccacggcc gtcgggagga acgtgaacgc ctggaggagg
     2701 gcgaacgaga ccacgagcgc cgcgaacagg cgcctcgagt cggtcgtcac agacccccca
     2761 cggttcaact gcagtccttt cccctgctcc cggccgccct cggccgggga tgctctggta
     2821 aatatcggca caccccggcg ggcattgagg aggaaaaggg ggcgctgctc agagtgcgga
     2881 gggaacgaag tcacccactg cggacgaacg ggtcgtgacg tcgcgcccgc gtcgcttcga
     2941 gtcatagagc gcctggtcgg ccgcgcggac cagcccggcg gcgtcggagg cattgagggg
     3001 gagggtggcc acgcccgcgc tcgcggtgat cgccacgtcc tcgtcgaccg ccgcaacggc
     3061 tgcgcgcagc ctctcggccg ccgctatcgc ctggtcggaa gagcagcccg gcaggaggac
     3121 gacgaactcc tcgcccccgt agcgggccgc ggtgtcgaac tcgcgggagg cgtcgcgcag
     3181 cgcggccccc gtcgcccgga ggacgccgtc gcctacctgg tggccgtacc tgtcgttgag
     3241 gtccttgaag tagtcgatgt ccagcaacac gagcgtgagc tgctcgcccg tgcgcagcgc
     3301 ccgggcgacc tcaccgtcga gagcgtcctc gaacgaccgc cggttcgcga ggcccgtcag
     3361 cccgtcggtg tccgccatct tccggacctc ctcgagcagc caggcgttgc gcagtgccag
     3421 cgccgcgtgc gagctgaact ggccgatcgt cgacaggacc cggcgctcga gccggtgccc
     3481 tcgccgccgc tgctcgacga cgagcacgcc gagctggttg ccgtccgcga tcagcggcgt
     3541 cacgagcagg tcccgcgctc cgggcaggag ctccgcgatc acgccgtcgg tcccggggtc
     3601 gaggtcggtg acgagagtcg tctcgcggcc ttgcgcgacc tccagcagga ggccgcagtc
     3661 ctcgggcacc ggcgttccca caccctcggc ccccttcgtg gcgagcaccg tgagcgctcc
     3721 gggggggccg gcgacgacga ggccgcggcg gaacccgagc gcgtcgcaca cccggccgag
     3781 gcaggactcc gcgacgttcc gcgggtcgga gacgctctcg aactcgacac ccatctcgtt
     3841 cagggcttcg aggtcgttct tgcggcgccg gagctcgcgc tcgttgagga acgagaacag
     3901 cgcggtgccg agggcgacga cccagaacgc cgtgacgttg aagatcgagg gccggtactc
     3961 gacgagcccc tccatccccg gcatgtggag gctcggccct cgggggtcga gcagccccgc
     4021 gagctgcgcg tggaacacca cgaagaacag cagcgagtgc cacagcgcga tcttcaggcc
     4081 ggtccggtac gacgcgagca gcgtcaccgc gatcaggtgc aggtagagca tgaagcgcag
     4141 cgggctctgc acgaagcccg tcagatacat gatccacgcg aggtacacgc cgtcggcgag
     4201 gagcatgccg gcgacgacgt agagatggcg tccgccgccg atcctgcgga ccgcctcgat
     4261 gacgccggtc agcacgaagt acgcgaacga cacgaccgcg acgtcgcgga gcgacgcgcc
     4321 cacgaagccg gggaacaaga gcgcggaggc catcgcgacc gcgacgaacc ccgcgcgcag
     4381 accctggaga tatcccatcc gctccgcgag cgagacgatc tcggccgagc gcccggtgct
     4441 gctccctctg cccctcattc gaaatacacc atgtccttcg acgagtcgac cggggcgaac
     4501 gcgagcttcg ggtcgcgccg gtcgatctcg ccctggatca acaggaagat cacgacgagg
     4561 atcgtcagcg agagcgggaa ggcgaacgtc ttgacggcct cgcccgcggt gcgggcgaga
     4621 tccgcgatcg aggcggtgaa cccgttgcgg gtgccgcccg gggtccccac gccaccggac
     4681 gtcgcgcttc cgtcgccggt caagtcgatc agccggcgcg gcggctcgtc ctcgccggtg
     4741 ccgttgccgg ggccggtcgg gtcgtccttg cgctcgccgg ttccctcgcc cgcgtcctcg
     4801 ccaccgaagt ccgggcgggg attcggtgtg gcgagcggcg ccagcgggcc ggggcccggg
     4861 ggcggcgtcg tgaagtccgg ggtcggaagc ggcggctccg gcggcagcgt ctgctcggga
     4921 ggcggcgtag acccgatctc gaccggcgcc ggcggcagct cgggaggcca cacgggcggc
     4981 ctgaagctcg gcacgacctc gggggtcggc gtcgggtccg gggtgggggt aggcgtcggc
     5041 tccggcgtcg gggtctcggt cggctccggg gtggggggcg gaggcggcgg agggggctcc
     5101 gtcggctcca cggtcgcgat cggcgtcggg gtcggctc
//
LOCUS       DASQDO010000209         5751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_694470, whole genome shotgun sequence.
ACCESSION   DASQDO010000209 DASQDO010000000
VERSION     DASQDO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694470"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /gene="selA"
                     /locus_tag="VEV43_06005"
     CDS             complement(<1..1105)
                     /gene="selA"
                     /locus_tag="VEV43_06005"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYP23107.1"
                     /translation="MSGELNERMRRLPSVDRVMGALSGVSPRVAAAAARRALDDARAR
                     VAAGGDAPGVEEVADMARASIERSRLRGLVPVVNATGVLIHTNLGRAPLGPEQLDAVV
                     EVSRGYSSLEYDVVEGRRGTRYGHARASIAELTGAEDALVVNNCAAAVLLVLSTFCAG
                     REVLISRGELIEIGGEFRIPDVMTASGAHLVEVGTTNRTHLADYERAITPETAAIFKV
                     HPSNYRVVGFTASVPGRELARLARGRGVPFFHDLGSGLVAAPETEWDAREGAEDEPLV
                     SSALADGADLVTFSGDKLLGGPQAGVVAGRADLVARLAKSPLLRAMRVDKMTLAALEV
                     TLRLHLEGRGRDVPVGAMAAAAPEDLERRAHSLC"
     gene            complement(1108..1773)
                     /locus_tag="VEV43_06010"
     CDS             complement(1108..1773)
                     /locus_tag="VEV43_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP23108.1"
                     /translation="MSSPQPIRVVLVDDHDLLRRGIKTMLETEADIEVVGEGSDGTQA
                     LPLVEEHLPDVVLVDVIMPTKDGIEATRDVKDAFPNVGVVVLSGHDAQRFIFVALKAG
                     ASGYLLKTADLEQVILTVRAVAKGEALIDPNVATQVLTQFQSYQEEEVADVYQPLTPR
                     EREILQLMSEGLPNKTIASRLSISERTVTTHVANIYAKLHVNNRVSAIQEAMRRRILD
                     YNP"
     gene            1897..2868
                     /locus_tag="VEV43_06015"
     CDS             1897..2868
                     /locus_tag="VEV43_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYP23109.1"
                     /translation="MTSPALRRVLAVVAVVAALTAFAAPAFAYDFERPLKRGDEGSDV
                     RALQTRIAGWFPRDDKTPMGIDGAFGRKTARAVKAFQRFYGMEVNGVAGAAVFEKLAS
                     LEDDDGSTAHFDWSEFVQNKNAGCSAKANAYAGTFGGGPIAPSVVKTNVRRLMWRLEA
                     LRAKTGGRPIGINSGFRSVAYNDCIGGARASQHMYGTAADNRIAEVDNRTGREVAMTS
                     QFSGVGCYSSLSHNHLDIRVENAGTGTTQFWWWPETDGKGRHLADDGLPCWGETVRKK
                     LGVARTTTTVLRSVAAALPGAGSLVPLPAEVEAFEDAGEPEDLLGLD"
     gene            2941..4065
                     /locus_tag="VEV43_06020"
     CDS             2941..4065
                     /locus_tag="VEV43_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23110.1"
                     /translation="MNERSLVLLIGGGLVAAIALAVVAYLLLDGGDDEESTGGSTATG
                     GNAASSLEGGPDEVLYLSGTSLIRRNLENGEERPVGMIESPSVYPSRGSQWIAYVTSK
                     KVRDDDFAAEPVLHLYDVETEEESTHGAGVAPVWNPSGTRVAFLRPVEPRECQGETCS
                     GDVQIGVIDTASGSESLLLEPGKYSILGWAGDWVLVSDFTNPATVISVSLDDEQKVLD
                     LPASQFWDASPDGAWVVKTNAKKTELIAIEDGELGDERVEVDLGEYQLFEGGWAHDSS
                     RVAAVVAEAPGEGRGAGAAPTSRVVTFSPDDPGLAEVGDSFGATGTILWSVDNDSIVF
                     ATLLDPKRALFQAKHCPLGNESGCEIVTSWTEGVMLLRAE"
     gene            4237..>5751
                     /locus_tag="VEV43_06025"
     CDS             4237..>5751
                     /locus_tag="VEV43_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23111.1"
                     /translation="MTAPFVDDTIAEDLPPASPDDKEMALAFQRGEKGAYQLIYERYE
                     DRVQRVCRRMLGKPEDAQEAAQEAFLRVYTALGKFNGRYHLGAWITRIATNVCLDHIR
                     ARSRRPVEPTPIEELELDFVASGESDPETVTVRNAESRRVRKVLLGLPPLHRAAIVLR
                     DFEGLSYAEVAVALGMTEGQVKALIHRARKGFRRSWTPLAEMLVPARLLQRWRETDVT
                     IKEHVVNAAASQSAPVASICSGVLQQCGTFVVHKMVPLGTTLILGGGSALAGIGSPPV
                     PPVEDAVVRAAQGETGDSPGELRAAPRRDAVAEDTPQTAPPEPSATPPTVTASPEPTE
                     PPAPSESPSALPATASPPPPSGTVTPRETTATPAPFAPELYFQQGQIPSRSAPAAHTV
                     RVDCATGAIDQTMSAHVSDHEGSYPIELRLKTGSTFEITFTVQKKGFAVNYTGGGVLI
                     SRSNSGNESYLTFNGRYGSGGTAADSAGLPVNGSIRIELALDCSAQSLITESVVL"
BASE COUNT          877 a         1982 c         2030 g          862 t
ORIGIN      
        1 cgcacagcga gtgcgcccgc cgctcgaggt cctcgggggc cgccgccgcc atcgccccca
       61 ccgggacgtc gcggccgcgg ccctcgaggt gcagcctgag cgtcacttcc agggcggcga
      121 gggtcatctt gtcgacgcgc atggcacgga gcagcgggct cttcgcgagc cgtgcgacga
      181 ggtcggcgcg gccggcgacc acgcccgcct gggggccgcc caggagcttg tcgccggaga
      241 acgtcaccag gtcggcgccg tcggcgagcg ccgacgagac caggggctcg tcctcggccc
      301 cctcccgcgc gtcccactcc gtctcggggg ccgcgacgag gccggagccg aggtcgtgga
      361 agaaagggac cccgcgcccg cgcgccaggc gggcgagctc acgtccgggg accgacgccg
      421 tgaaccccac gacgcggtag ttcgacgggt ggaccttgaa aatcgcggcg gtctcggggg
      481 tgatcgcccg ttcgtagtcg gcgagatgcg tccggttcgt cgtcccgacc tcgacgaggt
      541 gagcgcccga ggcggtcatg acgtcgggga tccggaactc gccgccgatc tcgatcagct
      601 cgccgcggct gatcaggacc tcgcggccgg cgcagaaagt cgagagcacg aggagcacgg
      661 ccgccgcgca gttgttgacc acgagtgcgt cctcggcccc cgtcagctcc gcgatcgacg
      721 cgcgcgcatg cccgtaacgc gtcccccgcc gtccttcgac gacgtcgtac tcgaggctcg
      781 agtagccgcg cgagacctcc acgaccgcgt cgagctgctc ggggcccaac ggggcgcggc
      841 ccaggttcgt gtggatcagc acgccggtcg cgttgacgac ggggacgagc ccgcgcagcc
      901 gcgaccgctc gatcgacgcg cgcgccatgt cggctacctc ctcgacgccg ggagcgtcgc
      961 cgccggcggc gacgcgcgca cgggcgtcgt ccagcgcgcg gcgcgctgcg gccgcagcga
     1021 cgcgcgggga gacgccggac agcgcgccca tgacgcggtc gaccgacggg aggcggcgca
     1081 tccgctcgtt gagctcgccg ctcatctcta cgggttgtag tcgaggatgc gccggcgcat
     1141 ggcttcctgg atcgccgaga cgcggttgtt cacgtggagc ttggcgtaga tgttcgcgac
     1201 gtgggtcgtg accgtccgct cggagatcga gagacggctc gcgatcgtct tgttggggag
     1261 gccctcgctc atcagctgga ggatctcgcg ctcgcgcggc gtcagtggtt ggtagacgtc
     1321 ggcgacctct tcctcctggt agctctggaa ctgcgtgagc acctgcgtcg cgacgttcgg
     1381 gtcgatgagc gcttcgccct tcgccaccgc gcgcaccgtg aggatgacct gctcgagatc
     1441 ggcggtcttc agcaggtagc cggacgcgcc ggccttcagc gcgacgaaga tgaagcgctg
     1501 cgcgtcgtgg ccggagagga cgacgacgcc gacgttcggg aacgcgtcct tgacgtcgcg
     1561 ggtggcctcg atcccgtcct tggtcggcat gatcacgtcg acgagcacga cgtcggggag
     1621 gtgctcctcg accagcggca gcgcctgggt gccgtcggat ccttcgccga cgacctcgat
     1681 gtcggcctcg gtctcgagca tggtcttgat cccgcggcgc aacaggtcgt ggtcgtcgac
     1741 gagaacgacg cgtatcggct gcggggatga catgccaagg atgataagcg ccccctcacg
     1801 gacgcggctt tacaccccgg ccgagggttt ttcccgaccc ggccgggacg gttttgtgat
     1861 tcaaggtcat cggcgtcttc cgccgattcc tctcctatga ctagtcctgc actccggcgc
     1921 gtcctggccg tcgtcgccgt ggtcgcggcc ctgacggcgt tcgcggcgcc agcgttcgcg
     1981 tacgacttcg agcgcccgct gaagcgtggc gacgagggct ccgacgtccg tgctctccag
     2041 acgcggatcg cagggtggtt cccgcgcgac gacaagacgc ccatggggat cgacggggcc
     2101 ttcggccgca agaccgctcg cgccgtcaag gcgttccagc gcttctacgg catggaggtc
     2161 aacggcgtcg cgggcgcggc ggttttcgag aagctcgcct cgttggagga cgacgacggc
     2221 tcgaccgccc acttcgactg gtccgagttc gtgcagaaca agaacgccgg ctgctccgcc
     2281 aaggcgaacg cctacgcggg cacgttcgga ggggggccca tcgcgccctc ggtggtgaag
     2341 acgaacgtcc gccgtctcat gtggcgcctc gaggctctgc gtgcgaagac cggcgggcgt
     2401 cccatcggga tcaactccgg tttccgcagc gtggcctaca acgactgcat cgggggagcg
     2461 cgggcgagcc agcacatgta cgggaccgcc gccgacaacc gcatcgccga ggtcgacaac
     2521 cggacggggc gcgaggtcgc catgacgagc cagttctccg gtgtcggctg ctacagctcg
     2581 ctgagccaca accacctcga catcagggtc gagaacgcgg gcacggggac cacgcagttc
     2641 tggtggtggc ccgagaccga cgggaagggc cggcatctcg ccgacgacgg cttgccgtgc
     2701 tggggcgaga ccgtcaggaa gaagctcggc gtcgcacgca cgacgacgac ggtgcttcgc
     2761 agtgtggccg cggcgctgcc gggtgccggg tccctcgtgc cgctcccggc ggaggtagag
     2821 gcgttcgagg acgcgggcga gcccgaggac ctgctggggc tcgactagct atccgtttcg
     2881 tccggcaccc ccttatggtt gctgactcgc ttaccaaagg tggtgtcccg agacgtgcgt
     2941 atgaacgaga ggtcgctcgt cctcctcatc ggtggagggc tcgtggccgc gatcgccctg
     3001 gcggtcgtcg cgtacctgct gctggacggc ggggacgacg aagagagcac cggcgggtcg
     3061 acggcgacgg gcggcaacgc cgcctcctcg ctggaagggg ggccggacga ggtcctctac
     3121 ctcagcggca cctcgctgat ccggcgcaac ctcgagaacg gcgaggagcg tcccgtcgga
     3181 atgatcgaga gccccagcgt ctacccgtcg cgcggctccc agtggatcgc gtacgtgacc
     3241 tcgaagaagg tccgggacga cgacttcgcg gccgagccgg tcctccacct gtacgacgtc
     3301 gagaccgaag aggagagcac gcacggcgcc ggagtcgcgc ccgtgtggaa cccgagcggc
     3361 acccgcgtgg cgttcctgcg gcccgtcgag ccgcgcgaat gccagggcga gacctgctcg
     3421 ggtgacgtgc agatcggcgt gatcgacacg gcctccggga gcgagtcctt gctcctcgag
     3481 cccgggaagt actcgatcct cggctgggcc ggggactggg tcctcgtcag cgacttcacg
     3541 aaccctgcga cggtcatctc ggtctcgctc gacgacgagc agaaggtcct cgacctgccg
     3601 gcgagccagt tctgggacgc ttcgccggac ggggcctggg tcgtcaagac gaacgccaag
     3661 aagaccgagc tgatcgcgat cgaggacggc gagctgggcg acgagcgggt cgaggtcgac
     3721 ctcggggagt accagctctt cgaagggggg tgggcccacg actcctctcg cgtcgcggcc
     3781 gtcgtggcgg aggcgcccgg cgagggccgc ggggccggcg ccgccccgac gtcgcgcgtc
     3841 gtgacgttct cgcccgacga ccccgggctc gccgaggtcg gggactcgtt cggggcgacc
     3901 gggacgatcc tgtggtccgt cgacaacgac tcgatcgtgt tcgcgaccct gctcgacccg
     3961 aagagggcgc ttttccaggc gaagcactgc ccgctcggga acgaatccgg ctgcgagatc
     4021 gtcacctcgt ggaccgaggg cgtcatgctc cttcgagccg agtagcgcgc acgacggaat
     4081 atcccgccgg cgcgtaacga atcccggccc gcggcgttat cggttgacga ggaaaggcag
     4141 gtccgcgggc gacgctgccc ggcccggatt tggtcctttc ggactattca ggcgagcgcc
     4201 cccgggtgtc agtctgggac cgtagatgag gactccatga ccgctccctt cgtggacgac
     4261 acgatcgcag aagaccttcc tcccgcctcc ccggacgaca aggagatggc gctcgcgttc
     4321 cagcgcggcg agaagggcgc gtaccagctc atctacgagc ggtacgagga ccgcgtccag
     4381 cgcgtatgcc gccggatgct cggaaagccc gaggacgccc aagaggccgc ccaggaggcc
     4441 ttcctgcgcg tctacaccgc gctcggcaag ttcaacggcc gctatcacct cggtgcgtgg
     4501 atcacgcgga tcgccacgaa cgtctgtctc gaccacatcc gtgcgcggtc gcggcgaccc
     4561 gtcgagccga cgccgatcga agagctcgag ctcgacttcg tcgcatccgg agagagcgac
     4621 cccgagaccg tcaccgtccg caacgccgag agccggcgcg tgcgcaaggt gttgctcggg
     4681 ttgcctccgt tgcaccgcgc agcgatcgtg ctgcgcgact tcgaagggct ctcgtacgcg
     4741 gaggtcgcgg tcgcgctcgg tatgaccgag ggccaggtga aggcgctcat ccaccgagcg
     4801 cgcaagggct tccgccgctc gtggacgccg ctggccgaga tgctggtgcc ggcgcggctg
     4861 ctccagcggt ggcgtgagac cgacgtcacg atcaaggagc acgtcgtgaa cgcggccgcg
     4921 tcacagagcg cgcccgtagc gtcgatctgc agcggcgtcc tgcagcagtg cgggacgttc
     4981 gtcgtgcaca agatggtgcc gttgggaacc accctcatcc tcggaggtgg gtctgcgctg
     5041 gccggcatcg gttcgccgcc ggtgccgccc gtcgaggacg ccgtcgtccg cgccgctcag
     5101 ggagagaccg gcgactcgcc cggcgagctc cgcgcggcgc cccgccgcga cgcagtcgcc
     5161 gaggacacgc cccagacggc cccccccgaa ccctccgcga cgccgcccac ggtgactgcg
     5221 tcacccgagc cgacggagcc gccggccccc agcgaatcgc cgtccgcgct tcccgcgacc
     5281 gcgagcccgc cacctccttc cggcactgtc acgccgcgcg agacgaccgc aacgcctgcg
     5341 ccgttcgcgc cggagctgta cttccagcag gggcagatcc cgtcccgttc ggctcccgcc
     5401 gcgcacaccg tgcgcgtcga ctgcgcaacc ggcgcgatcg accagacgat gagcgcgcac
     5461 gtctcggatc acgagggctc gtatccgatc gagctccggc tgaagaccgg ctcgacgttc
     5521 gagattacgt tcacggtgca aaagaagggc tttgcggtca actacaccgg tggtggtgtc
     5581 ttgatctcgc gaagcaattc aggcaacgag agctacctta cttttaatgg aaggtatggg
     5641 agtggcggca ccgccgcgga ctccgcagga ctgccggtca acggttccat ccggatcgaa
     5701 ttggccctag actgctcggc gcagtcgctg ataacagaga gcgtcgttct c
//
LOCUS       DASQDO010000210         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_756390, whole genome shotgun sequence.
ACCESSION   DASQDO010000210 DASQDO010000000
VERSION     DASQDO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756390"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VEV43_06030"
     CDS             complement(<1..271)
                     /locus_tag="VEV43_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23112.1"
                     /translation="MSSRGPYRDAVAWMRSRVPSAQVRGAVAGGALAAALIDGLLLMS
                     VIGLVSPSSFGVSGGRAFGSTSRTVTLVAVAGACAVWLVLHLRTHR"
     gene            412..1398
                     /locus_tag="VEV43_06035"
     CDS             412..1398
                     /locus_tag="VEV43_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYP23113.1"
                     /translation="MSIQVKDYEGGAPTWMVWGALWVIYIVWGSTYLAIRVTVETLPP
                     FLSAGARFFLAGAIVYAVLALRKGVAAIRITKRELGASTLVGAALLFGGNGLVSVAEQ
                     KVSSSLAALIIASVPLWVILLRTLTGEKVSRSTLVGVAIGFVGVAMLVLPGGDQSANV
                     AGLLMLVVASLSWASGSFFSSKLPLPRDPFVSTAAQMVTGGAVLLVAGLVTGEASGLD
                     PAEFSGASLSAFVYLVTVGSLFAFTCYVWLLQNAPISKVATYAYVNPVIAIFLGWLIL
                     SESITTSILVGAAVIVSSVAFIVSRESAKPRPESDDADAPAEAALAGASSSS"
     gene            complement(1395..2570)
                     /locus_tag="VEV43_06040"
     CDS             complement(1395..2570)
                     /locus_tag="VEV43_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23114.1"
                     /translation="MKSLRALLAGIALAVTAAVPSSAHGCHVPAPAGSPSECGLATVA
                     SVEVSEAAGAGRVEVHGKIAAVLRRDEGIVSLVDVSKPGRPKVLGSYDGGLDGQRAGK
                     SLDGDLAFSSDGQWLFYARQSTQMSADGLHVVDVSDPSAPTLRFYQPEGGTFRVDVYE
                     TETATYVITLDAIAGLVVNRFDPATGALTTVFSDPEPASRRVGGPASAGIVIDRKDPK
                     LGIPVLYTSSGTTGLQVYDLSTPDQPVLLGSWAETGLAEIELVRTKKSRTVYAATEYW
                     FDETLEPEVVVLDAKDLGAIKEKKRFSLNVPAAEEFRLQGMAINKGRLYAAHSSLGLV
                     GFDLKGHPVAYLSDLGERNQSSTELSATYAYDVEASKGLLYVTDAASGTLTLARRTR"
     gene            2694..2852
                     /locus_tag="VEV43_06045"
     CDS             2694..2852
                     /locus_tag="VEV43_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23115.1"
                     /translation="MPRKVFPLLMAAGLMLGATGVACDKEDRKDVQEVGNDVDKEVDQ
                     LDSDGKDD"
     gene            complement(2849..>3619)
                     /locus_tag="VEV43_06050"
     CDS             complement(2849..>3619)
                     /locus_tag="VEV43_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HYP23116.1"
                     /translation="AAVDVARELYMGGSNDAIEAVLIAEDFSDVQSRLAYLRTSQQAQ
                     SEVFERLAADRSALESKLTQLEKDRAAAMAAEERLLALRTEIEGKLVDQQDEIAELNA
                     AIERAERREARAAAAAEEEAAAATPVVAAPPAPAPAPNGNAQTAVEAALSQVGKPYQW
                     AAAGPDSYDCSGLTMWAWAQGGVALPHNSGMQYAATPRVAQSDWQPGDLLFFGSPIHH
                     VGMYIGNGQMVEAPYTGEFVRVVSASPSDYVGAGRPGS"
BASE COUNT          505 a         1254 c         1273 g          587 t
ORIGIN      
        1 agcgatgcgt ccgcaggtgc aggacgagcc atacggcgca cgcgcccgcg accgcgacca
       61 gcgtgaccgt ccgagacgtg ctgccgaagg cgcggccccc ggacacgccg aagctcgacg
      121 gcgacaccag accgatcacc gacatcagca ggagcccgtc gatcagagcg gcggcaagcg
      181 ccccgcccgc caccgccccc cgcacctgcg cggaaggaac ccgcgacctc atccacgcca
      241 cggcgtcccg gtacggccct cgtgaagaca tcgtccggag cgtagcgggg gcggccttcg
      301 tttcttaggc tcgagcgccc gatcgtccga gttcgcgcca ccccgttttg cgattcttcg
      361 aagttcccgg gggcccggaa tacgcccccg cggcgcccgg ttgccgccga cgtgagcatc
      421 caggtgaagg actacgaggg cggcgcgccc acctggatgg tctggggcgc cctctgggtc
      481 atctacatcg tctgggggtc gacgtacctc gcgatccgcg tcacggtcga gaccctcccg
      541 ccgttcctca gcgccggcgc gcgcttcttc ctcgccgggg ccatcgtcta cgcggtgctg
      601 gccctgcgaa aaggagtcgc cgcgatccgc atcactaagc gggagctcgg cgcctcgact
      661 ctcgtggggg ccgcgttgct gttcgggggc aacgggctcg tctcggtcgc cgagcagaag
      721 gtctcgtcga gcctcgccgc gttgatcatc gcctcggtcc cgctgtgggt gatcctgctc
      781 cgcacgctga ccggcgagaa ggtgtcccgc tcgacgctcg tcggcgtcgc gatcggtttc
      841 gtgggcgtcg cgatgctcgt gctgcccggc ggcgaccaga gcgcgaacgt cgccggcctg
      901 ctgatgctcg tcgtggcttc gctctcgtgg gcctcgggct cgttcttctc gagcaagctc
      961 ccgctgccgc gcgacccgtt cgtgtcgact gccgcgcaga tggtgaccgg cggggcggtg
     1021 ctgctcgtcg cgggcctggt caccggcgag gcgtcggggc tcgacccggc ggagttctcc
     1081 ggcgcgtccc tgagcgcgtt cgtctacctc gtcaccgtcg ggtcgctgtt cgccttcacg
     1141 tgctacgtgt ggctgctcca gaacgcgccg atctcgaagg tcgcgaccta tgcgtacgtg
     1201 aaccccgtga tcgcgatctt cctcggctgg ctgatcctgt cggagtcgat cacgacctcg
     1261 atcctcgtgg gggccgcggt catcgtgtcg tcggtagcgt tcatcgtgag cagggagtcg
     1321 gcgaagcccc ggcccgagtc cgacgacgcg gacgcgccgg cggaggccgc tctagcgggg
     1381 gcttcttctt cgagctagcg agtgcggcgc gcgagcgtca gcgttccgct cgccgcgtcg
     1441 gtcacgtaca gcaggccctt cgaggcttct acgtcgtacg cataggtggc gctcagctcg
     1501 gtcgacgact ggttgcgctc gccgaggtcc gacaggtatg cgacggggtg gcccttgagg
     1561 tcgaacccga cgagcccgag gctcgagtgc gcggcgtaga gacggccctt gttgatcgcc
     1621 atgccctgca ggcggaactc ctccgccgcg gggacgttga gcgagaaccg cttcttctcc
     1681 ttgatggcgc cgaggtcctt cgcgtcgagc acgacgacct cgggctccag cgtctcgtcg
     1741 aaccagtact cggtcgcggc gtagacggtg cgtgacttct tcgtccggac gagctcgatc
     1801 tcggcgagcc cggtctcggc ccacgagccg agcaacaccg gctggtcggg cgtcgacagg
     1861 tcgtacacct gcaggccggt cgtgccgctg gacgtgtaga gcaccgggat gccgagcttg
     1921 gggtccttgc ggtcgatgac gatgcccgcg gatgccgggc ccccgacgcg gcgactcgcc
     1981 ggctccgggt cggagaacac cgtcgtgagc gccccggtcg ccgggtcgaa gcggttgacg
     2041 acgagaccgg cgatcgcatc gagcgtgatg acgtaggtag cggtttcggt ctcgtacacg
     2101 tcgacgcgga acgtgccgcc ctccggctgg tagaagcgga gcgtcggcgc ggacggatcc
     2161 gagacgtcga ccacgtgaag gccgtcggcc gacatctgcg tggactgtcg cgcgtagaag
     2221 agccactggc cgtcggaaga gaaggcgaga tcgccgtcga gcgacttccc ggccctctgc
     2281 ccgtcgagcc cgccgtcgta ggagccgagc accttcggcc gtccgggctt gctcacgtcg
     2341 acgagcgaga cgatgccttc gtcacgccgg aggaccgcgg cgatcttccc gtggacctcg
     2401 acgcgcccgg cgccggcagc ttcggagacc tcgacggacg cgaccgtcgc gagcccgcac
     2461 tccgaggggg agccggcggg agcggggacg tggcacccgt gcgcgctcga agggacggcc
     2521 gccgttacgg cgagcgcgat tccggcgagc aatgcgcgta gggacttcat gcgagaggag
     2581 attcgccgcg ggccgggcct tccctccgga cgacgttacg tagcggattg aatgcttagc
     2641 gtggtgaaaa ccgggtatcc ccgggctagg accgaccacg gaaggaggtt cggatgcccc
     2701 gcaaggtgtt tccgctgctt atggccgccg gcctgatgct cggcgcgacc ggcgtcgcct
     2761 gcgacaagga agaccgtaag gacgtccagg aggtaggcaa cgacgtcgac aaggaggtcg
     2821 accagctcga ctccgacggc aaggacgact agctgcctgg tctaccggct cctacatagt
     2881 ccgaagggct cgccgagacc accctcacga actcacccgt gtagggggcc tcgaccatct
     2941 ggccgttgcc gatgtacatc ccgacgtggt ggatcgggct tccgaagaac agcaggtcgc
     3001 ccggctgcca gtcggactgc gcgacccgag gggtcgccgc gtactgcatc ccggagttgt
     3061 gggggagcgc cacgcccccc tgcgcccagg cccacatcgt caggcccgag cagtcgtacg
     3121 agtccgggcc cgcggccgcc cattgatacg gcttgccgac ctgtgacagc gcggcctcga
     3181 ccgcggtctg ggcgttgccg ttcggggcgg gggccggtgc cggcggagcc gcgaccaccg
     3241 gggtggcggc agcggcctct tcctctgccg cggcggcagc ccgggcttcg cgtcgctccg
     3301 cccgttcgat cgcggcgttc agctcggcga tctcgtcctg ctggtcgacg agcttcccct
     3361 cgatctcggt gcgaagggcc agcaggcgct cctcggcagc catggccgcg gcccggtcct
     3421 tctcgagctg cgtgagcttg ctctcgagcg cggaccggtc ggcggccagc cgctcgaaca
     3481 cctcggactg cgcctgctgt gaggtgcgga ggtaggcgag ccgcgactgg acgtcgctga
     3541 agtcctcggc gatcagcacc gcttcgatcg cgtcgttcga gccgcccatg tagagctcgc
     3601 gcgccacgtc gaccgcggc
//
LOCUS       DASQDO010000211         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1123270, whole genome shotgun sequence.
ACCESSION   DASQDO010000211 DASQDO010000000
VERSION     DASQDO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123270"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VEV43_06055"
     CDS             complement(<1..949)
                     /locus_tag="VEV43_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23117.1"
                     /translation="MKHLTSRRWLAGATAALIAASIAPAHAGTRNLHERLQKATRYLA
                     MNQEDDGSISGGFSRLGTTSDAILAFASEMRGKPQVRDAMGWLEEHVEDATTLGLKAK
                     LVLAAVAVGRDPRSFGGRDLVQEIMDTHDPETGQYGGESDTEVGYHALAMLAVMAGRE
                     DPPESASRWLRDAQCDDGGWQFDEPAGPNDDEHCFDSTAENDFSRSDTNTTAYADMAF
                     EGSPGPDPKVDPISFLRTARDDYKKGFVYDPHGKCTEETLGEQFCYLTDANSTGLVLQ
                     AMLGEVKAKEFVRYRDALMKLQYPRCGKWMGAFAFTWIYD"
     regulatory      complement(1060..1239)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1204..1704)
                     /locus_tag="VEV43_06060"
     CDS             complement(1204..1704)
                     /locus_tag="VEV43_06060"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HYP23118.1"
                     /translation="MGLAALIVYVTLGRRRIDYRFVLIGAVLPDLAAAWPGSHSLVVV
                     ALLALVAMVLPLREKRLALFGLPVGWLLHLVGDGMWDYPRVFLWPAFGADVPVHPEPY
                     SWDLVADPLAHLSTWGAELAGLLLLAWFWVAFGLGHQGRARLFLKDGYLRPWDGPGDT
                     VGADNP"
     gene            complement(1716..2168)
                     /locus_tag="VEV43_06065"
     CDS             complement(1716..2168)
                     /locus_tag="VEV43_06065"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HYP23119.1"
                     /translation="MRGFLHADGGARGNPGPAGIGVVLKDGDGNVIGEIARGIGVATN
                     NVAEYTALIEGLELALDKGVTDVGVYMDSKLVVFQVNGDWKIKNDQLRRLAVKAHSLL
                     RRFERKTLKHVPRERNSAADKLANQGMDAAALDAELDAEASVRHDPLF"
     gene            complement(2176..>2450)
                     /locus_tag="VEV43_06070"
     CDS             complement(2176..>2450)
                     /locus_tag="VEV43_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HYP23120.1"
                     /translation="GVVEGLTGEISGELETHTTARTTISAEIPADLLKLYEQLRAQKG
                     GVGAAGLEGGTCLGCHTKLPQRELERMRAEGGLQRCDNCRRILVIE"
BASE COUNT          368 a          859 c          831 g          392 t
ORIGIN      
        1 cgtcgtagat ccaggtgaac gcgaaggccc ccatccactt gccgcagcgc gggtactgga
       61 gcttcatcaa cgcgtcgcgg tagcggacga actccttggc cttgacctct cccagcatcg
      121 cctggagcac caggccggtc gagttcgcgt cggtcaggta gcagaactgc tcgccgagcg
      181 tctcctcggt gcacttgccg tgcgggtcgt acacgaaccc cttcttgtag tcgtccctgg
      241 cggtccgcag gaacgagatc gggtcgacct tcgggtcggg gccgggagat ccctcgaacg
      301 ccatgtccgc gtacgcggtc gtgttcgtgt ccgagcggga gaagtcgttc tcggcggtcg
      361 agtcgaagca gtgctcgtcg tcgttcggcc cggcgggctc gtcgaactgc caccccccgt
      421 cgtcgcattg agcatccctg agccaccggg aagcgctctc cggtgggtcc tctcttccgg
      481 ccatcaccgc gagcatcgcg agcgcgtggt atccgacctc ggtgtcggac tcgcctccgt
      541 actgaccggt ctcgggatcg tgcgtgtcca tgatctcctg cacgaggtcc cggcccccga
      601 acgacctcgg gtccctgccg acggcgaccg ccgcgagaac gagcttcgcc ttgagcccga
      661 gcgtggtcgc gtcctcgacg tgctcctcga gccatcccat ggcgtcccgc acctggggct
      721 tgccgcgcat ctcggaggcg aaggcgagga tcgcgtcgga ggtcgtcccc agccgcgaga
      781 agccgccgct gatcgagccg tcgtcctcct ggttcatcgc gaggtaccgg gtcgccttct
      841 ggagccgttc gtggaggttc cgtgttcccg cgtgtgcggg ggcgatcgag gcggcgatca
      901 aggccgcggt ggcgcctgcg agccacctcc tcgaggtgag gtgcttcatg cctgctcctt
      961 cccggccccc ggtcctcgcg gcggccacga aaaacgaccc ctccgaagaa ggggtcgcga
     1021 gcgtggcgtg aagccccggc tctccctttg cctcgaaggt cgtcgagccg cacgccgagc
     1081 aggttttctg gctcgcccgc cctacgaccg gccgcctcgg cgggcggtca acacgtaaag
     1141 ggcggaccta cagttgcggg acagcgccgg aatccgaccg gacttgccct gcgaagcgtg
     1201 cagttatggg ttgtcggcac ccactgtatc gcctgggccg tcccacggcc ggaggtaccc
     1261 gtctttcaag aacaaccggg cgcgcccctg gtggccgagc ccgaacgcga cccagaacca
     1321 cgcgagcaac aagaggccgg cgagctcggc cccccacgtc gagagatgtg ccaaggggtc
     1381 ggcgacgagg tcccacgagt acggctcggg gtggacgggg acgtccgcgc cgaacgcggg
     1441 ccacagaaag acgcgcgggt agtcccacat gccgtcgccg acgaggtgga ggagccaccc
     1501 gaccgggagc ccgaacagcg cgagcctctt ctcacggagc ggaaggacca tggcgacgag
     1561 cgcgagcagc gcgacgacca ccaacgagtg agagccgggc caggccgcgg cgagatcggg
     1621 aagcacggca ccgatcagca cgaagcggta gtcgatgcgg cgccggccca aggtgacgta
     1681 gacgatcagg gccgcgagcc ccatgtgcca caggatcaaa acaacgggtc gtgccggacc
     1741 gacgcttcgg cgtcgagctc cgcgtccagc gcggctgcgt ccatcccctg gttcgcgagc
     1801 ttgtcggcgg ccgagttccg ctcgcgcggc acgtgcttca gcgtcttccg ctcgaagcgg
     1861 cgcagcagcg agtgcgcctt caccgcgagc cgccgcagct gatcgttctt gatcttccag
     1921 tcgccgttca cctggaagac gacgagcttc gagtccatgt agacgccgac gtcggtgacg
     1981 cccttgtcga gcgcgagctc gaggccctcg atcagcgcgg tgtactcggc gacgttgttc
     2041 gtcgcgacgc cgatcccgcg cgcgatctcg ccgatgacgt tgccgtcacc gtctttgagg
     2101 acgacgccga tcccggcggg gcccgggttg ccccgcgctc caccgtcggc gtggaggaac
     2161 ccgcgcacgt cgtagctact cgatgacgag gatccggcgg cagttgtcgc aacgctggag
     2221 accgccctcc gcccgcatcc gttcgagctc gcgctgcggg agcttcgtgt ggcagccgag
     2281 acaggtcccg ccttcgaggc ccgcggcccc cacgccgccc ttctgcgccc gcagctgctc
     2341 gtagagcttc aggaggtcgg ccgggatctc cgccgagatc gtcgtgcggg ccgtcgtgtg
     2401 ggtctcgagc tcgccggaga tctcgccggt gaggccctcg acgacgccgt
//
LOCUS       DASQDO010000212         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_450130, whole genome shotgun sequence.
ACCESSION   DASQDO010000212 DASQDO010000000
VERSION     DASQDO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450130"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VEV43_06075"
     CDS             complement(<1..533)
                     /locus_tag="VEV43_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HYP23121.1"
                     /translation="MTTKSKVPYRCSFCGKSQEQVRKLIAGQGVYICDECINLCQEII
                     EEEMLEAPRASLAQATPLPSPRRIKDQLDTYVISQEQAKKVLSVAVYNHYKRVNAGHS
                     VDDVELGKSNVLLVGPTGSGKTLLAQTLARVLDVPFCIADATSLTEAGYVGEDVENIL
                     LRLIQAADFDVQRAQRG"
     gene            601..1107
                     /locus_tag="VEV43_06080"
     CDS             601..1107
                     /locus_tag="VEV43_06080"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYP23122.1"
                     /translation="MNVRLRFAEVDTELGPMLVARTSEGVAAMSRGLNVASFLAALEL
                     RYPGAEPAEDGRALASDAAWLDGYLAGDRRDLPRVDLRGLRAWDRAVYEAVRAIPYGA
                     TATYGEVAVTAGSPGGARAVGGSLSRCPLFPAVPCQRVVLAGDGISGWGGGDIALKRR
                     LLDMEARR"
     gene            complement(1104..>1751)
                     /locus_tag="VEV43_06085"
     CDS             complement(1104..>1751)
                     /locus_tag="VEV43_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HYP23123.1"
                     /translation="VAASDDPTGPFRDLGRALVADAFAIDAHPFRDDDGTWYLYYARD
                     FLDGERVGTALVVDRLVDMQSLAGDPTTVLRATADWQIYQRARPMYGAVYDWHTLEGP
                     FVVKRHGRYWCFYSGGAWREDNYGVSYVVASSPTGPFVEPPSDGPTVLRSVPGRVVGP
                     GHCSVVTDRAGDDWLVYHAWDPDRTARRMHIDRLEWTPDGPRSDAPTWTPQRPPG"
BASE COUNT          252 a          599 c          639 g          261 t
ORIGIN      
        1 atgccgcgct gcgcgcgctg gacgtcgaag tccgcggcct ggatcagccg caggaggatg
       61 ttctcgacgt cctcccccac gtacccggcc tccgtcagcg acgtcgcgtc cgcgatgcag
      121 aacgggacat cgagcacgcg agcgagcgtc tgcgcgagca gcgtcttccc ggagcccgtc
      181 gggccgacga ggaggacgtt cgacttcccc agctcgacgt cgtcgacgct gtggccggcg
      241 ttgacccgct tgtagtggtt gtagaccgcg accgaaagga ccttcttcgc ctgctcctgg
      301 ctgatgacgt acgtgtcgag ctggtccttg atgcggcgcg ggctggggag cggcgtggcc
      361 tgcgcgaggc tggcgcgcgg cgcctcgagc atctcctcct cgatgatctc ctggcagagg
      421 ttgatgcact cgtcgcagat gtacacgccc tggcccgcga tcagcttgcg gacctgctcc
      481 tggctcttgc cgcagaacga gcagcggtac gggaccttgg acttcgtcgt catcggacgc
      541 cgaggatagc acgggcgttc cgctgctccg ccaccgcacg atcgtcatga ttcgggtccc
      601 atgaacgtcc ggctgcgctt cgccgaggtc gataccgagc tcggcccgat gctcgtcgcg
      661 cgcacgtccg aaggcgtcgc ggcgatgtca cgcgggctga acgtggcgag cttcctcgcg
      721 gcgctcgagc tcaggtatcc cggcgcggag ccggcggagg acggacgcgc gctggcgagc
      781 gacgccgcgt ggctcgacgg gtacctcgcg ggtgatcgac gggacctgcc gcgcgtcgac
      841 cttcgcgggc tgcgtgcatg ggaccgcgcg gtgtacgaag cggtgcgcgc gatcccgtac
      901 ggcgccaccg cgacgtacgg cgaagtcgcc gtgaccgccg ggtcaccggg aggagcacgc
      961 gccgtcggag ggtcgctctc gcgctgccca ctcttcccgg ccgtgccgtg tcagcgggtc
     1021 gtccttgccg gggacgggat ctcgggatgg ggcggcggcg acatcgcgct caagcggcgt
     1081 ctcctcgaca tggaagcccg gcgctagccc ggcgggcgct gcggagtcca ggtcggcgcg
     1141 tcggagcgag gcccatccgg cgtccactcg aggcgatcga tgtgcatgcg tcgcgccgtg
     1201 cggtccggat cccaggcgtg gtacacgagc cagtcgtcgc cggcgcggtc ggtgacgacc
     1261 gagcagtgcc cagggccgac gacacggccg ggcacagagc gcagcacggt cggtccgtcc
     1321 gacggcggct cgacgaacgg cccggtcggc gacgacgcga cgacgtacga gacaccgtag
     1381 ttgtcctcgc gccaggcgcc gcccgaatag aagcaccagt agcggccgtg gcgcttgacg
     1441 acgaacgggc cctcgagcgt gtgccagtcg tagaccgccc cgtacatcgg ccgcgcgcgc
     1501 tggtagatct gccagtcggc agtggcacgc agcacggtcg tgggatctcc cgccagagac
     1561 tgcatgtcca cgagccgatc gacgaccagg gcggtaccca cgcgctcgcc gtcgaggaag
     1621 tcacgcgcgt agtacaggta ccaggtgccg tcgtcatccc ggaacgggtg ggcgtcgatc
     1681 gcgaaggcgt cggccacgag cgcgcggcca aggtcccgga acgggcctgt cgggtcgtcc
     1741 gaggccgcga c
//
LOCUS       DASQDO010000213         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_756602, whole genome shotgun sequence.
ACCESSION   DASQDO010000213 DASQDO010000000
VERSION     DASQDO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756602"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1106)
                     /locus_tag="VEV43_06090"
     CDS             complement(180..1106)
                     /locus_tag="VEV43_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HYP23124.1"
                     /translation="MAAELNVAALAARAGSRVLEWWLSPLPRRRVAWLRVLVYGFIFV
                     DVFLLRPWVGDNGLVPGDQYHPLMVGRLLPLPTPTPLLVEVVKYSMLACAAIAASGRF
                     VRLAGALVFGLYLEWMLIAFSYGKVDHDRVAFLVALAVLPTVGKVAWGDSTSDEKAAW
                     AIRCIQIAVVATYFLSAFAKFRFGGFDWVNGATLLRAVIRRGTDLAEPLMSMPWVLRM
                     GQWGIVLFELASPLMLERGRIGRVFLVSAFVFHTITFATIEIVFWPHVVCLLAFLPLE
                     RFVPAHAQGQRSGPGASRTNRPRKALLLTRAR"
     gene            complement(1091..1516)
                     /locus_tag="VEV43_06095"
     CDS             complement(1091..1516)
                     /locus_tag="VEV43_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23125.1"
                     /translation="MSIWMRTRIAAATAAFVLLCVGTFWGDDDNFPFGPFRMYSTKQE
                     LDGEVRAIEVYGLTRSGAWVGVPFDDFGLRRADIEGQLGMLAVPPEEVLASMAVAYDR
                     FDRGDQEVLAFRLVERTYDVEDGVPAGETVEFLATWRRS"
     gene            1723..>2445
                     /locus_tag="VEV43_06100"
     CDS             1723..>2445
                     /locus_tag="VEV43_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23126.1"
                     /translation="MAWGEPFGDTGTVATHVWLSPPPSGEDQGEIVYAGAFGDGDVIF
                     RIALDDITGPPVAVETDPEFTNIFCAGHCWLTDGRLLVAGGQLPGLADEEEDQHEHGG
                     MAGGGERACWIYHPRTRAWIRTGSLNLAPDGAPNSGGRWYPTCVTLPDGRVLAVGGHP
                     DIREEYHDPPNAPGHRHNNNIPEMFSPSTETWTYLPDVRSAPAGRVTDSYPRIHMLPN
                     GTVFFATDPGGTKRHFDPYGETF"
BASE COUNT          440 a          870 c          801 g          334 t
ORIGIN      
        1 actgcggtcg aagtgatacc ggaggaagtc cgcttcacga cgttccacgt gatccgcccc
       61 gacggcgaag gtttctcagg gggcgcggcc gtcgccgaga ccctgtccgc catagcgtgg
      121 acgtcggtcc tcggccgcgt cctcgggcct cggcccctgc gccccgtcgt cgagctcttc
      181 taccgggctc tcgtcaagag caaagccttc ctaggacggt tcgtgcggga cgcgccgggc
      241 ccggaacgct ggccctgagc gtgtgccggg acgaaccgct cgagcggtag gaacgccagc
      301 aggcacacca cgtgcggcca gaacacgatc tcgatcgtcg cgaacgtgat cgtgtggaac
      361 acgaaggccg acacgaggaa gacgcggccg atccgtcctc gttccagcat cagcggcgac
      421 gcgagctcga agagcacgat gccccactgg cccatgcgca ggacccacgg catcgacatg
      481 agcggctccg ccagatccgt cccgcgccgt atcaccgctc gcagaagcgt cgcgccgttg
      541 acccagtcga agcctccgaa gcggaacttc gcaaacgccg acaggaagta cgtcgccacg
      601 actgcgatct ggatgcacct gatcgcccac gccgccttct cgtcggacgt cgagtccccc
      661 cacgcgacct tgcccaccgt gggcagcacc gcgagcgcga cgaggaacgc gaccctgtcg
      721 tggtcgactt tgccgtacga gaaggcgatc agcatccatt cgaggtagag gccgaagacg
      781 agcgcgccgg ccagcctgac gaagcgcccc gaggccgcga tcgccgcaca cgccagcatc
      841 gagtacttga cgacctcgac gagcagcggc gtcggtgtcg ggagcgggag cagcctcccg
      901 accatcaacg ggtggtactg gtcgccgggg acgagcccgt tgtcgccgac ccagggacgg
      961 agcaggaaga cgtcgacgaa gatgaagccg tacacgagca cgcgcaacca cgctacgcgc
     1021 cggcggggga gaggcgacaa ccaccactcg aggacccgcg acccggctcg tgcagcgagc
     1081 gccgccacgt tcagctccgc cgccacgtgg cgaggaactc gacggtctcg ccggccggca
     1141 cgccgtcctc gacgtcgtac gtccgctcga cgagacggaa ggcgaggacc tcttggtcgc
     1201 cgcggtcgaa acggtcgtac gcgaccgcca tcgacgcgag gacctcttcc ggaggcacgg
     1261 ccagcatccc gagctggcct tcgatgtcgg cacggcgcag gccgaagtcg tcgaagggga
     1321 cgcccaccca cgcgcccgac cgggtaagcc cgtagacctc gatcgcgcgt acctcgccgt
     1381 cgagctcctg cttggtcgag tacatgcgga acggcccgaa cgggaagttg tcgtcgtcgc
     1441 cccagaacgt gccgacgcac aacaggacga aagcagccgt agccgccgct atgcgtgtcc
     1501 gcatccagat cgacaccgtc cgctcccccc gaccctccgg gcatcgacag gaaggaaagc
     1561 tacgacctcg ggcgatctag ttcgtgccgt gcctcgaccc cggcctccga acccacggtg
     1621 aggcgatggg acctcgaaaa aagacgtcgc cgtaagacct cgccttgtcc ggttgccgtc
     1681 cgggtgagaa ggttgtcctt cgggcgagca cgggggcgga ctatggcttg gggcgaaccg
     1741 ttcggcgaca ccggcaccgt cgcgacacac gtgtggcttt cgccgcctcc ctccggcgag
     1801 gatcagggag agatcgtcta cgcaggcgcc ttcggggacg gcgacgtcat cttccggatc
     1861 gctctcgacg acatcaccgg cccgccggtc gccgtggaga ccgaccccga gttcacgaac
     1921 atcttctgcg cgggccactg ctggctgacc gacggacggc tgctcgtcgc gggcggccag
     1981 ctccccggtc tcgccgacga ggaagaagat cagcacgagc acgggggcat ggccggcggg
     2041 ggcgagcgcg cgtgctggat ctatcaccca cgcacgcggg cgtggattcg cacgggctcg
     2101 ctgaacctgg ctcccgacgg tgccccgaat agcgggggcc gctggtaccc gacctgcgtg
     2161 acgctgcccg acgggcgggt cctcgcggtc ggcgggcacc ccgacatccg cgaggagtac
     2221 cacgatccgc cgaatgcacc gggtcaccgg cacaacaaca acatcccgga gatgttctcg
     2281 ccttccacgg agacctggac gtacctgccg gacgtccgaa gcgctcctgc cggacgggtg
     2341 acggactcct acccgcggat ccatatgctt ccgaacggca cggtcttctt cgccacggac
     2401 cccgggggca cgaagcgaca cttcgacccc tacggggaga ccttc
//
LOCUS       DASQDO010000214         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_511488, whole genome shotgun sequence.
ACCESSION   DASQDO010000214 DASQDO010000000
VERSION     DASQDO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511488"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VEV43_06105"
     CDS             <1..1274
                     /locus_tag="VEV43_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23127.1"
                     /translation="FAGGALAPLRAERRTPAPAPAGVDRAALVGPGGNETSDGAIAAL
                     TERTEANPARADVHAQLGLAYLQHARDEAEPASLPLAEAALERSLQLRPRRNLEAFLG
                     MAALANARHDFSGSIDWSRRAIATSPYDASAYGLLGDASFELGKVGAADAAYQEMVDR
                     RPDVASYVRASYALQYHGRSKAATSAMELALEAAGPSGETAAWVRHQMGDVYAGLGDH
                     REAARQNRIGIEIAPGYAPPMVGLAEAHIARGRLERALEIVEAAAAELPSIEYLVTLG
                     DLYRATGHEDEARTQYDAVTRKLAEYRRNGILPDADFILFYADHGVRPGAALREAFSI
                     YRDRPTPKTADALAWMLHSRGRDRAAWPYAAEALRAQPRDASTAFHAGSIARALGRDT
                     VARRLARRAITIDPYFSVVQAPTARRIAGRA"
     gene            1271..1642
                     /locus_tag="VEV43_06110"
     CDS             1271..1642
                     /locus_tag="VEV43_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23128.1"
                     /translation="MTPKRWRAGAVACVAAASLVGCSGEPPSFDDLSGLAEAVNGEGV
                     VCDGVTRKQGTDLVDEIGACRGSDVTLYVFESTAALDDWRKVAALVAPTAVGANWAVT
                     GDPAAVERVADALGAEMAPAE"
     gene            complement(1658..>2305)
                     /locus_tag="VEV43_06115"
     CDS             complement(1658..>2305)
                     /locus_tag="VEV43_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYP23129.1"
                     /translation="GLVSVIDYTFASPMNFKPLAIGFDVCFHSATKYLNGHSDVVAGC
                     AAGGRSAIESIRRSLNQLGGSLDPHAGFLLARSLKTLALRVRAQNENASKLAGFLEDD
                     PRVAEVHYPGLVSHPDHERGRRLMSGFGGMLSFRLAGTSPGEVDEVLARLGIAYVATS
                     LGSVETLVTRPAATSHAALTVEQRERVGITDDLVRVSCGVEDADDLVADFDQAMA"
BASE COUNT          345 a          833 c          812 g          315 t
ORIGIN      
        1 tgttcgccgg cggcgcgctc gcgcctctcc gggccgaacg ccgcaccccg gcgccggccc
       61 ccgccggcgt cgaccgggct gcgctcgtcg ggcccggagg caacgagact tcggacggcg
      121 cgatcgctgc gctgacggag cgaaccgaag cgaaccctgc tcgcgcagac gtgcacgcac
      181 agctcgggct cgcgtatctc cagcacgcgc gagacgaggc ggagccggca tccctgccgc
      241 tcgccgaggc cgcgctcgag cgatcgctgc agctgcgccc acgacggaac ctcgaagcgt
      301 tcctcggcat ggcagccctc gcgaacgccc gccacgactt ctcgggatcg atcgactggt
      361 cacgccgggc catcgccacc agtccctacg acgcctccgc ctacgggctt ctcggagacg
      421 cgtcgttcga gctcggcaag gtgggggctg ccgacgccgc gtaccaggag atggtcgacc
      481 gccgtcccga cgtcgcgtcg tacgtgcgtg cgtcgtacgc gttgcagtac cacggcaggt
      541 cgaaggcggc gacgagcgcg atggagctcg cgctcgaggc cgcggggccg agcggcgaga
      601 cggcggcgtg ggtccgtcac cagatgggcg acgtctacgc cgggctcggt gaccaccgcg
      661 aagccgcgcg acagaaccgc atcgggatcg agatcgcgcc cggctacgcg ccgcccatgg
      721 tggggctggc cgaggcgcac atcgcccgtg gccgtcttga gcgggcgctc gagatcgtcg
      781 aggccgccgc ggccgagctc ccctcgatcg agtacctcgt cacgctcggc gatctctacc
      841 gcgccaccgg ccacgaggac gaagcccgga cgcagtacga cgccgtcacc cggaagctcg
      901 ccgagtaccg ccgcaacggg atcctgcccg acgccgactt catcctcttc tacgcggacc
      961 acggcgtacg gccgggggcc gcgctacggg aggcgttctc gatctatcga gaccgtccca
     1021 ctcccaagac cgccgacgcc ctggcgtgga tgttgcactc acggggacgc gaccgcgcgg
     1081 cgtggccgta cgcggcagag gccctccgag cgcagccccg tgacgcatcc acggcgttcc
     1141 acgccggatc gatcgcgcga gccctgggcc gcgacaccgt tgcacgtcgt ctggcgagaa
     1201 gggcgatcac gatcgatccc tacttctcgg tcgtccaggc gccgacggcg cgccggatcg
     1261 cggggcgggc atgactccga agcgctggcg cgctggcgcg gtcgcttgcg tcgccgccgc
     1321 ctcgctcgtc ggttgctcgg gagagcctcc gagcttcgac gacctgtcgg gcctggcgga
     1381 agcggtgaac ggggaaggcg tggtgtgcga cggcgtcact cggaagcaag gaaccgacct
     1441 cgtggacgag atcggcgctt gcagagggag cgacgtgacc ctgtacgtgt tcgagagcac
     1501 cgcggcgctc gacgactgga ggaaggtcgc ggccctcgtg gcgcccacgg ccgtcggcgc
     1561 gaactgggcg gtgaccggtg accccgcagc cgtggagcgc gtggccgacg ctctgggtgc
     1621 ggagatggca cccgccgagt aatccggaaa ggcgccctta cgccattgcc tggtcgaagt
     1681 ccgcgacgag gtcgtcggca tcctctacgc cgcagctcac acgcacgagg tcgtccgtga
     1741 tcccgacccg ctcgcgttgc tccaccgtca gcgcggcgtg cgacgtggcg gcggggcgcg
     1801 tgaccagcgt ctcgacgctg ccgaggctcg tcgcgacgta cgcaatcccg aggcgcgcga
     1861 gcacttcgtc cacctctcca ggcgacgtcc cggcgaggcg gaagctcaac atgccgccga
     1921 accccgacat gagccttcgg ccgcgctcgt ggtccgggtg ggagacgagg cccggatagt
     1981 gcacctcggc gacccgggga tcgtcctcga ggaagccggc gagcttcgac gcgttctcgt
     2041 tctgggcccg caccctcaga gcgagcgtct tcagcgaacg cgccagcagg aagccggcat
     2101 gggggtcgag gctgccgccg agctggttca gcgacctccg tatcgactcg atcgcgctcc
     2161 ggcccccggc ggcgcacccc gcgacgacgt ccgagtgccc gttgaggtac ttggtcgcgc
     2221 tgtgaaagca gacgtcgaac ccgatcgcga gcggcttgaa gttcatcggg ctcgcgaacg
     2281 tgtagtcgat caccgacacc agccc
//
LOCUS       DASQDO010000215         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_756688, whole genome shotgun sequence.
ACCESSION   DASQDO010000215 DASQDO010000000
VERSION     DASQDO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756688"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VEV43_06120"
     CDS             complement(<1..649)
                     /locus_tag="VEV43_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYP23130.1"
                     /translation="MWFDSHCHLFDVEGDPSQVVGRAAEVGVTDMLVCGTDVPTSEQA
                     VALAELPGVWAAAGLHPTSAKEWDDGVAAALEALLGRDRVVAVGESGLDMYWDTSYLD
                     LQQEVFEVHVGWAKTHDKALVIHTRSSVDEALAALERLSPPDRLVFHCWSGDETQMRR
                     ALDLGSFVSFAGNVTFKSAGDLRELAALVPGDRLLVETDSPYLAPVPHRGKRNEPA"
     gene            902..1189
                     /locus_tag="VEV43_06125"
     CDS             902..1189
                     /locus_tag="VEV43_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYP23131.1"
                     /translation="MKSTGVVRKIDELGRIVLPSELRRVFGIREGDELEISVDGDKVI
                     LQKRQDLCLFCGASNPAVEFKERRVCESCAGELGTMGRREVRLPEDEVINR"
     gene            complement(1277..2095)
                     /gene="rsmI"
                     /locus_tag="VEV43_06130"
     CDS             complement(1277..2095)
                     /gene="rsmI"
                     /locus_tag="VEV43_06130"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYP23132.1"
                     /translation="MVLCGTPIGNLDDMSPRAVQALRDADVIACEDTRRTRKLLSHFA
                     IGARDLVVLNEQTERRRAPEVVAEIGRGRTVVLVSDAGMPGLSDPGYRLVQACIAAGV
                     TVDVVPGPTAVVTALAVSGLPPGRFVFEGFLPRKPGDRRKRIESLKDEPRTIVLFESP
                     HRIEATLEDLLAVLGDRPAALARELTKLYEEVLRGPLSEVAAALRVKPPRGEITLVVE
                     GAVHDHKPEPDAEELAARARSLMDAGVDRKEAMARVAAEAGVPRRKVFDALLDD"
     gene            complement(2170..2340)
                     /locus_tag="VEV43_06135"
     CDS             complement(2170..2340)
                     /locus_tag="VEV43_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23133.1"
                     /translation="MNVVAISDHVGAVNDSRAVSAARRFFAEQPVASDAASWRGALAA
                     ALRYAFEPWRPR"
     gene            complement(2371..>2968)
                     /locus_tag="VEV43_06140"
     CDS             complement(2371..>2968)
                     /locus_tag="VEV43_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23134.1"
                     /translation="GLGAATLYDRYVRETPPVARARSDATFALLGGADSTSRTGALSD
                     IDPRDFGFDARSGVVLSYDPRSAAYSAADTHRDLREVAGAVAIQLGELDQPVYVLGHS
                     QAAGVLDRLDGASPQAAVVISPPPKRVPPVEVPPPGVRGPGKPGGDATRALAGLLEAA
                     GLGGFDIDAPSAPVHLDPAGGSDSGTRRMAVWALGDSV"
BASE COUNT          437 a         1047 c         1067 g          417 t
ORIGIN      
        1 gcgcgggctc gttgcgcttg ccgcggtgcg gcaccggcgc gaggtagggc gagtcggtct
       61 cgacgagcag ccggtccccc ggcacgagcg cggccagctc acgcaggtcc cccgcgctct
      121 tgaacgtcac gttcccggcg aaggagacga acgagccgag gtccagtgcc ctccgcatct
      181 gggtctcgtc gcccgaccag cagtggaaga cgagccggtc cgggggcgac agccgttcga
      241 gggccgcgag ggcctcgtcg accgagctac gggtgtggat gacgagggcc ttgtcgtggg
      301 tcttcgccca cccgacgtgg acctcgaaga cctcctgctg caggtcgaga taggacgtgt
      361 cccagtacat gtcgagcccg ctctcgccca cggcgaccac ccggtcacgc cccagcaagg
      421 cctccagggc cgcggcgacc ccgtcgtccc attccttggc ggaggtcggg tggaggcccg
      481 ccgcggccca cacccccgga agctcggcca gggcgacggc ctgctccgaa gtcgggacgt
      541 cggtcccgca gacgagcatg tcggtgacgc cgacctcggc cgcccgtccg acgacctggg
      601 acgggtcgcc ctcgacgtcg aagaggtggc agtgggagtc gaaccacacg gctcagacct
      661 tccccgcgga agaaaacgtt tcgcgagtca tccaggaatc tccaattcga gcgcttccaa
      721 aagctcccaa atcagggcgc ttcggccaaa ttgctgaccg gattgaccgg tttgatgttt
      781 gacttccgcc ctacagccgt tcaagatatc cattacttac cagtaactgc ctgcatcgtt
      841 gggagcggtg caagctgtgg cggcgctgct gggagatggg gaagcaaggg gtctgactgg
      901 gatgaagtct acgggagtag tgcgcaagat cgacgagctg ggccgaatcg tcctcccgtc
      961 cgagctgcgc cgcgtcttcg ggatccgaga gggtgacgag ctcgagatca gcgtcgacgg
     1021 cgacaaggtc atcctgcaga aacggcagga cctctgcttg ttctgcggcg cctccaaccc
     1081 ggcggtcgag ttcaaggagc gccgggtgtg cgagtcgtgc gccggcgagc tcgggacgat
     1141 gggacgccgc gaggtgcggc ttccggagga cgaggtcatc aaccggtaac gcttccggcg
     1201 gtaccggagc cgcatccagt accaggtttt cgaacggggg tcgcctcagg gcggcccctt
     1261 gtcgcgcccc ggccggtcag tcgtcgagga gcgcgtcgaa caccttccgg cgcggcaccc
     1321 cggcctccgc ggcgacccgg gccatcgcct ccttcctgtc gacgccggcg tccatcagag
     1381 agcgcgcccg ggcggccagc tcctcggcgt ccggctccgg cttgtggtcg tgcacggccc
     1441 cctcgacgac gagcgtgatc tcgccgcgcg gcggcttcac cctcagcgcc gcggcgacct
     1501 cggagagggg gccgcgcagc acctcctcgt agagcttcgt cagctcccgg gcgagggccg
     1561 cggggcggtc gcccaggacg gcgaggaggt cttccagcgt cgcctcgatg cggtgcggcg
     1621 actcgaacag gacgatcgtg cgcggctcgt ccttcagcga ctcgatccgc ttgcggcggt
     1681 cgcccggctt gcgaggcagg aacccctcga acacgaagcg gcccggcggc aggccgctga
     1741 cggcgagagc cgtgacgacc gcagtcgggc cggggacgac gtcgacggtg acgcccgcgg
     1801 cgatgcaggc ttggacgagc cggtagccgg ggtccgacag gcccggcatc ccggcatcgg
     1861 acacgagcac gaccgtgcgg ccgcggccga tctcggcgac gacctcgggg gccctgcggc
     1921 gttcggtctg ctcgttgagc acgacgaggt cgcgggcccc gatggcgaag tgcgacagca
     1981 gcttgcgggt ccggcgagtg tcctcgcagg cgatcacgtc ggcgtcgcgc agcgcctgga
     2041 ccgcgcgcgg cgacatgtcg tcgaggttcc cgatgggagt cccgcagagg accaacctcc
     2101 cttttccttt gggcgtcccg ccgaggcggc ccgttgcttt tggcgtcccg ccgaggcggc
     2161 ccgcgccggt catcttggcc tccacggctc gaaggcataa cggagcgcgg ccgccagcgc
     2221 accgcgccac gacgccgcgt cggaggccac cggctgttcg gcgaagaacc gcctcgccgc
     2281 ggacactgca cgcgagtcgt tcaccgcgcc gacgtggtcg gagatcgcga cgacgttcac
     2341 ctcgcccggg cgacgccagt ccccctgcag tcacaccgag tccccgagtg cccacacggc
     2401 catccggcgg gtcccggagt cgctcccgcc ggcggggtcg aggtggaccg gcgcggaggg
     2461 ggcgtctatg tcgaagccgc cgaggccggc cgcctcgagc agccccgcca gggccctcgt
     2521 cgcgtcgcct cccggcttcc ccggcccccg cacccccggt ggagggacct ccacgggcgg
     2581 gacgcgcttc ggcggcggtg agatgacgac ggccgcttgg ggcgacgcac cgtcgaggcg
     2641 gtcgagcacg ccggcggcct gggagtggcc gaggacgtac accggctggt cgagctcgcc
     2701 gagctggatc gccacggcac cggcgacctc tctcaggtcg cggtgcgtgt cggccgcgga
     2761 gtaagcggcg gaacgtgggt cgtacgagag aacgactccg gagcgcgcgt cgaagccgaa
     2821 gtcacgcggg tctatgtccg aaagagcgcc ggtccggctc gtcgagtcgg caccgcccag
     2881 cagcgcgaac gtcgcgtcgg accgcgcccg cgccacgggc ggcgtctccc tgacgtagcg
     2941 gtcgtacaag gtcgcggccc ccagcccg
//
LOCUS       DASQDO010000216        21638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_327773, whole genome shotgun sequence.
ACCESSION   DASQDO010000216 DASQDO010000000
VERSION     DASQDO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327773"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VEV43_06145"
     CDS             complement(<1..139)
                     /locus_tag="VEV43_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYP23135.1"
                     /translation="MSTQPSDRGITEASGGVATLTRSDFVSDQEVRWCPGCGDYAILA
                     AV"
     gene            complement(136..1989)
                     /locus_tag="VEV43_06150"
     CDS             complement(136..1989)
                     /locus_tag="VEV43_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYP23136.1"
                     /translation="MSSQVAPPKAGERREVDRVVIRFAGDSGDGMQLTGDRFTSVSAS
                     FGNDLATMPDFPAEIRAPAGTIAGVSAFQIHFSDHDILTPGDQPGVLVAMNPAALKAN
                     LKDLTRGGLLIVNSDAFEDRALDKVGYSANPLTDGSLEGYQVVQIPMTSMTVEAVKEA
                     GVSKKEAERSKNMLALGVICWMYSRPLEPTVAWLEKKFAKKPQIAQANVAALKAGHAF
                     GETAELVAYEVRPATLAPGRYRRITGNQAIAFGLIAASVHAKLPLFLGSYPITPASDI
                     LHELSRHKNFGVRTVQAEDEIAAISMAIGASFGGHLGVTTTSGPGLDLKSEALGLALS
                     LEIPLIVIDIQRGGPSTGLPTKTEQSDLLHAMYGRHGEAPLPIVASYSPPSCFWAAIE
                     SARLALKYMTPVILLSDGYLANGAEPWRIPAVEKLPDISVPFASQPNAGEDFWPYLRD
                     EITLARPWAIPGTPGLEHRVGGIEKADGTGNISYDPENHQLMTLLRQAKIKAIEEDIE
                     PLEWDGDDEAKVLVLGWGSTYGAIGAACRRLRLRGKKIARAHLKHLNPMPKNTEEVLR
                     RFETVVIPEMNMGQLSRLIRADFLIDTISINQVKGLPFRAGDLEERLLELV"
     gene            complement(2272..2571)
                     /gene="fdxA"
                     /locus_tag="VEV43_06155"
     CDS             complement(2272..2571)
                     /gene="fdxA"
                     /locus_tag="VEV43_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009409155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HYP23137.1"
                     /translation="MTYVIAEPCIGVKDRACVDECPVDCIYEGEEQLYIHPEECVDCD
                     ACLPACPVDAIFPADNIPAEWANFTQVNAQWFQDNPDASGGAMESPFAPSEERTE"
     gene            complement(2686..3156)
                     /locus_tag="VEV43_06160"
     CDS             complement(2686..3156)
                     /locus_tag="VEV43_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23138.1"
                     /translation="MRTDRGPLVAIALLLLAGVSCNRGGGAPAVPADCGVPSPDPAAR
                     PELIPEPFLLDGDAGVASAGRRKGGVTGVLSVELGVQEALPVYRKAVRAAGFDIVSED
                     NEGFEAEVYLKQGKRLGAIQIRRTLCDDASIVFVNIVEGNFAMPIVSTPSPSPP"
     gene            complement(3146..3958)
                     /locus_tag="VEV43_06165"
     CDS             complement(3146..3958)
                     /locus_tag="VEV43_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP23139.1"
                     /translation="MELRGRTGILTGASRGLGVELGRALASRGVHLALAARDEEGLRK
                     TAGQLEGSGVRTIVVPTDVTKVADLETLVARTTDELGPPDLLVNNAGIETIAPFATFD
                     MDRIDAIVETNLTAVMKLTRLVVPGMIERRRGHVLNVSSASGKSGVPYYGVYGATKHA
                     LVGFSWSLRAEMAQHGVGVSVLCPSFVSDIGMYADRSNNAKPPRSIATVTPPEVSAAA
                     LRAIEKNRAEVVVARGLAKVADVFYALSPDVSMKVARRMGVDAFLATTISED"
     gene            complement(3949..4806)
                     /locus_tag="VEV43_06170"
     CDS             complement(3949..4806)
                     /locus_tag="VEV43_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYP23140.1"
                     /translation="MRTGRFALPYVAGLTLLVGIPVAGAIALAFTDFSGVQDPEWIGF
                     DNFERLWDQPLFWKSVGNTAAFVAIAVPLRIAAAVALALLLHRRTAGSGAARAVAYLP
                     TVVPDVAYALLWLWLLNPLYGPIAAGASSLGLTAPGWLTEPWAARVSIAVMGAFQIGE
                     AFVVALAARRTVPEPLYEAARVEGARPWFTLRNLTLPVMAPVLGLLALRDVVLALQVN
                     FVPAFLVTEGGPAYATTFLPLYVYEAGFRYFRFGYASAVSVVMFVFTAAILALQYWIY
                     RRWRERRWS"
     gene            complement(4806..5810)
                     /locus_tag="VEV43_06175"
     CDS             complement(4806..5810)
                     /locus_tag="VEV43_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP23141.1"
                     /translation="MSIAVAGATKTYRSRAGSVVALEDVDLTAADGELLVVVGPSGSG
                     KTTLLRSIAGLEQLDEGTVQVAGRDVTKSPPGDRGVAMVFQEYALYPHMTVAANISFG
                     MRARKEGRGEIERKLESAARHLDLEDVLDRRPNELSGGERQRVALARAMVREPDAYLM
                     DEPLSNLDAKLRAQMRAEIRNLQRSVGATMLYVTHDQVEAMTMADRVAVLRDGLVEQV
                     APPVELYDQPATAFVARFVGNPPMNLIDAPPWDPDVTIGVRPEHVALVPPGAGRLDGR
                     IAEVEFTGNESVVHVDCGPTWVLVKVPRGFSPRLGAETGISFDESRIRRFSGRDGAAL
                     "
     gene            complement(5807..7081)
                     /locus_tag="VEV43_06180"
     CDS             complement(5807..7081)
                     /locus_tag="VEV43_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HYP23142.1"
                     /translation="MSRKLVALALLLTLVTAACSDSPDAAGEEDEGGVAHIELQVSGE
                     PEETAVYSSMAEQFMEANDGTHVEVVEIPEKDEHLARLATSFAGGNPPDVFLVNFREY
                     SQFVVRDAIEPIENHLDGVDLGAYYEPPLEAFTYDGALQCFPQNISSLVVYYNTALFE
                     TAGLKRPKAGWTWEDFRDTAATLTKGDVRGAGIEPTIIRLAPFAWSAGGDIVDDPDNP
                     TRLDLDNDGARTALGFFVSMVKDGLIPTEEEVAAQDLDTRFTTGKLGMLLSSRREVPA
                     FREVQGLEFDVAPLPVDQEPAGILHSDGFCMAAGSDQLDVAADFVEFATGEEGQTLGA
                     LSGRTVPSLSAVAESGAFLDPVQPPAHSKVFLDAIPTIRRTPVLSTWPEIEDVAEQIL
                     IRAFYEDGYSVDEAISDLDTETRGLFEEALQE"
     gene            complement(7093..7965)
                     /locus_tag="VEV43_06185"
     CDS             complement(7093..7965)
                     /locus_tag="VEV43_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYP23143.1"
                     /translation="MSRATGRLGERPRRTTGRVGERPGHRWARRIAIVAVSALFLAPL
                     AFMVAGSLRRPGLPPPDGFEFVPEVVQTGNYRDVTLFVPMWTYVRNSAFVVAVAVPVT
                     VLVASWAGFAIATAGRRTRRLLIGLSLVCLMIPVSSLWVPRFVMFEWAGILDTFVPLM
                     LPALMATSPFYVLLFALVYARIPRELFEAAALERLSPLATWRKIGWPLARPATFAVAM
                     LAFVFHWSSFVEPLLYLSEQSLYTVPLGLRSLHALEPTLHPILLAASVVVTLPGVVAF
                     FLAQRTFLTKTVGP"
     gene            complement(7962..8378)
                     /locus_tag="VEV43_06190"
     CDS             complement(7962..8378)
                     /locus_tag="VEV43_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23144.1"
                     /translation="MYRRGIALVAVPLFLSGCGGGDPAARPTPCPSRPAVEPPRDVPA
                     VLDQTHRGDVVEVGRRRGFSAVTVITQETVVELYPQLARGLVEGGHEITSGDNEGFEA
                     EIYFALRDGRAGRYVLREGPCRDDVTLHILYEERRR"
     gene            complement(8462..11251)
                     /locus_tag="VEV43_06195"
     CDS             complement(8462..11251)
                     /locus_tag="VEV43_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23145.1"
                     /translation="MKSLSTRALAGVLVLVVCASITVGALASPGPGTPAAALQCPPGS
                     RAVPVGDELREMTGEAAEIGGVVCSTPPKVPEPVAEMALAEGMRATIHGAPHGRVPAN
                     ALAKAVRRADALAARAARVPGTRGRWSPYGKGPLIANDPRFDEVNGAGFVHLQGRIDS
                     LGHDPRTNRLFASVGTGGVWMSRNLGESWKSIGDKLPTQIVGAIGWSKAKGGTLIAGS
                     GEPLMGGNTYTGLGAFYTSNLGKTWRRAKGVPNGAMAFKVAIDQAKQNIVYVATSKGL
                     YRSTNAGRSYRNVKLPTGPCKGKHTGKCLLANFVTDVVVQAPDNFENKGGEVLAAVGY
                     RAGARPFPQDPDTLESPNNGLYWSKNGKPGSFDKLEAPGFTPQHRIGRIELGAAVGPE
                     QNHDYVYAIVQDAELYNGAPPSSIDVPEEGGESPLPPNTNFNGIYVTADFGQTWTQMA
                     NTETIAENPATGSALVGVGQALFNGPGIQAWYNEWIAPDPTRQVNGIPTRLTFGLEEV
                     WQNTVTRQPLIGPSDFKVIGRYYAAETCIIGVGGIPTCPPENPSGITTVHPDQHDAIW
                     VSHGEGDLSLVIGNDGGAYVQTIQEDLELNQAGWGDGANTGFRTLLPYGAAMAKDGTV
                     WYGMQDNGSGKIEPDQKQYMTYGADGFYAAVDPDNSDVAYTESQGGGLRVTTDGGQTW
                     TDIGTGDSGHMFGNPFVMDPLDAKHLMTGGYALYERKEGPEGDWVNVFELGTEKNDVP
                     RQMSAVALRGKTAYAGFCGVCDIISSAEPFKNGIATNVGGKWHIAPAKGLANRYITDL
                     AIDPKNPKTVYVTLGGYANRQWRPPGSYGDENKQLGRGNVYRSTNGGRTFRSISRGLP
                     KAPAFTIIVRGKQLMIGTQIGAFLSKDKRGSAWAPAMRGLVRVPVNELQQAPQDKKLI
                     VAATFGRGVYLYRFP"
     gene            complement(11507..11914)
                     /locus_tag="VEV43_06200"
     CDS             complement(11507..11914)
                     /locus_tag="VEV43_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23146.1"
                     /translation="MVATTKRPDLVGLLVKSPAYGDDRVYLVDLDGVRRWVPSNDVLH
                     ALFRPGTPIISDIDAICIAPGDQLSTGAHLAQDESDKKVYLVDGVKRWVTSAAAMNRY
                     SFNYETVDPTKHETLAQLDDGPDIEGPSSEGAV"
     gene            complement(11979..13010)
                     /gene="lipA"
                     /locus_tag="VEV43_06205"
     CDS             complement(11979..13010)
                     /gene="lipA"
                     /locus_tag="VEV43_06205"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HYP23147.1"
                     /translation="MAPRTVIPTIRTRESIERDLRRGTPLHAAPGPEAPRPQRRPEWL
                     KVRVKTGENFSELRSIMRERGLHTVCEEAACPNIYECWEAREATFLLCGDLCTRRCSF
                     CDVMTAKPSALDPEEPAKIAEAVRLMGLRFAVVTGVARDDLDDAASAHWAATVRAIRA
                     AMPDCGIEILIPDFKGRRESPRASLERVIEARPDVLAHNLETVRRLHPAIRPGFGYEA
                     TLDLLRWAKEIRPDQVTKSNLIVGMGEREEEVYESMRDLRATGCDVLTIGQYLQPSVS
                     WHLPVDRWVEPAEFARYKAFGEDELDFAWVESGPLVRSSYHAGKQYARAAERLGALRP
                     TVTGNSATY"
     gene            complement(13012..13995)
                     /locus_tag="VEV43_06210"
     CDS             complement(13012..13995)
                     /locus_tag="VEV43_06210"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYP23148.1"
                     /translation="MPEKNVVTAIHDTLQEEMRRDDRVVVLGEDVGARGGVFRVTMGF
                     LDEFGEGRVIDTPLDEALIAGVGIGLAIDGLRPVAEMQFADFSFPGFNQIIQEAARIR
                     YRSNGDFSVPMVVRAPYGGGVHGALYHSQSVEAFYAHVPGLKVVVPSTPYDAKGMLIQ
                     AIEDEDPVIYLEHKKTYRLIKGDVPDERYTIPLGKAVVRREGTDLTCVSYGLMHHYCI
                     EAAERLSGEGLEVEVIDLRSIFPLDMDTVLESVGKTSKAMVVYEDNKFLGVGAEVAAQ
                     IAEKAMFELDGPIVRIGGPHVPAMPFAPPLEAWFMPDVDKIEQEMRKLAEF"
     gene            complement(14000..15007)
                     /locus_tag="VEV43_06215"
     CDS             complement(14000..15007)
                     /locus_tag="VEV43_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYP23149.1"
                     /translation="MAKGKDLHTDLGLKEEDLLEMYRYMLMARFCDERQWGLNRQGKA
                     PFVVPVSGHEAAQVGSGWAFERGKDVFCPYYRDMALVLVAGFTPKDVFLGLFGKKDDP
                     SSGGRQMPAHWGSRERNIITGSSPIATQCLHAAGIAYSKKLKKEDVVVGTWCGEGGTS
                     EGDWHGAMNFAGVHKLPLVFVVENNQYAISVHESKQLGGRVYKRAEGYGFPGFEVDGN
                     DVLECYKLSKEAVDRARAGEGPTLIEMRTYRHYSHTSDDDDRTYRKKEEVEEWKRKDP
                     IPRFEAYLKSVEMIDDGRIAELREKTKAEVAEGAKEAEQSDFPDPLSAGTNVYADLEV
                     V"
     gene            complement(15021..15731)
                     /gene="lipB"
                     /locus_tag="VEV43_06220"
     CDS             complement(15021..15731)
                     /gene="lipB"
                     /locus_tag="VEV43_06220"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HYP23150.1"
                     /translation="MSKTPPTALTASWLGRVEYPAAWSWQQELFLARLDGEIGDSLML
                     LEHPPTYTLGRRGTKEDLVYGDRERATRGISMYEVDRGGRATYHGPGQLVGYPILSLG
                     ERYDVISYLRKLEDVLIKVAGALGVEAHRDDDHTGVWVGRNKIGAIGVKITRGISMHG
                     FAFNVATDLSMFEGIVPCGIRDRWVTSVAAETGKIRPLKDVASLAALYLSETFDRTLV
                     WTHPRSLHRAGHLTAGAL"
     gene            complement(15728..17260)
                     /locus_tag="VEV43_06225"
     CDS             complement(15728..17260)
                     /locus_tag="VEV43_06225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYP23151.1"
                     /translation="MATEIKMPQLGETVVEGTITRWLKQEGDTIEEDELLVEISTDKV
                     DSEVPSSASGTIQKILVQEGDTVKVGTALAIVGEGAVEGGGGDAAEADQAETQAASSA
                     GVPEGDEGASEEVGDRAEQVAQQRQESGAAGDQGTQGSGGSQERMESEKADASATRKQ
                     EKKDLAPSSRPSTGGPSSRPGDSSKRGIISPLVRRLADEHDVDLDEIEGTGTGGRIRK
                     QDVLKYVQEGKARREARPSEQAEPAPRQEEPARRDAPPPPPPRREEAPAASGGAREEV
                     VPWSNIRRRTAEHMVAASHEAARAWNAVEADWTRVVALRTKAKERFRELEGFNLTYLP
                     FLARAVCDTLLEMPEVNATFDEEQQANVLKRYVNLGIAVALDGGGLIVPVVKGADEKN
                     LAGIARAINDVATRARSKKLTPDDLSGGTFTITNPGPFGSIMSVPIIPRGQTAILAFE
                     AIQKRVVVTDDDAIAIRSMGFLPMSWDHRTIDGAEAAKFLARLRERIETTDFAPELSP
                     YL"
     gene            complement(17498..18295)
                     /locus_tag="VEV43_06230"
     CDS             complement(17498..18295)
                     /locus_tag="VEV43_06230"
                     /inference="COORDINATES: protein motif:HMM:NF042666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HYP23152.1"
                     /translation="MRKLAAGLFAMALAITGVACGEEGGGGGGDSVDALALAADKATE
                     AGTATMTMTMEMSAAGQTVSVDAEGELDFANELAEMTMTMAVPGTPGEQTIKMVMEGN
                     YIYMELPPELGGTGDWVRMDASAIPGAATNPQMSQDPSQYLEFLRGASEDGLEEVGTE
                     KIDGDETTHYKAELSFEKILDQAPDEEAADEIRAQLEQFGGEVDSIPADVWIDEEGLP
                     RQMKLDMSMEAQGQSIEMLITMGFSDYGAEVDIEPPADYEEIEAPTG"
     gene            complement(18354..19778)
                     /gene="lpdA"
                     /locus_tag="VEV43_06235"
     CDS             complement(18354..19778)
                     /gene="lpdA"
                     /locus_tag="VEV43_06235"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HYP23153.1"
                     /translation="MANGNNAFDVVILGGGNAGYAAAFRAEALGLTVAMVEKDKVGGT
                     CLHRGCIPTKALLHAGELTDEIREAGNFGVIVQEPQVDWGKVLNFKDSVVGRMYKGLS
                     GLVSRKKNLELVEGEGRLTDAKTVTVTTKDGDRKLTANKGVVLATGSFPRDLPFIAAD
                     GDKVQNSTHGLSAQDIPSSVVVVGGNYIGLEFASVYRSFGAEVQVVEMLPKIAPAEDA
                     DISEALHKLLQKRGIKFHLGASVAGATKTDSGVEVEIEKNGERKTLAADRMFVAIGRG
                     PNTEGMGFEEAGIKTDKGFIVVDQQFRTSVDGVFAIGDAIWVPDANWPHPQLAHVAYA
                     EGMRVAELLAGESPTAVDYRNVPHVIYCQPEVAAVGLTEDKAKELGMDVEIKRYPFSG
                     NARALMLGGGQGFVKTVAEKGGAVVGVHIVGPRASDLIAEAMLVTSWEAYPSELAEII
                     HPHPTLTEVVGETFLDLAGKPLHG"
     gene            19903..20121
                     /locus_tag="VEV43_06240"
     CDS             19903..20121
                     /locus_tag="VEV43_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23154.1"
                     /translation="MRKMRVLVATMGAALAMTLFPASAQAAHHCAYPPIDDTEGVVYM
                     VWLTCEYGYHDPVGVALYAACWLSPTCP"
     gene            complement(20058..20990)
                     /locus_tag="VEV43_06245"
     CDS             complement(20058..20990)
                     /locus_tag="VEV43_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HYP23155.1"
                     /translation="MRDGNGTRRADLAGLAARQHGAVSLRQLEALGYSRSAVSRRARD
                     GSLRRVFPGVYRIGGARVTPELRAVAALLWAGPDAVASHETAAALWGMTGAAPELHVT
                     APRRLTSPPCGVQAHVATLAKRDRGKLRAVAVTSPARTLLDVASRCGGDSLLTLVERS
                     VIDGIVSPAQLRDVVGRNPGRRGSRRLIQALGPATSSALERRVEAILRGSRLPPFVRE
                     HEVGPYRLDFAWPDRRVGLEADGRRWHSSAPDFERDRAKHNFLVDRGWQVWTVTWRDL
                     DDPARCLSSIEKGLYGQVGDSQHAAYSATPTGSW"
     gene            complement(21054..21551)
                     /locus_tag="VEV43_06250"
     CDS             complement(21054..21551)
                     /locus_tag="VEV43_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HYP23156.1"
                     /translation="MLSKSLGAAMNEHLSKDLYAAHLYLAMSSNFESNNLPGSAACMR
                     AQADEERTHGMKFYEYIYDRGGSVELMSLDKPPATFKSPLDAFQQAHEHERHVSEAID
                     GLYRQALDEGDYASQVFLQWFIQEQVEEEKTSGTLVETLKMVGDSTASLLLFDRELGS
                     RGAGS"
BASE COUNT         3157 a         7521 c         7384 g         3576 t
ORIGIN      
        1 ggaccgctgc gaggatcgcg tagtccccgc agcccgggca ccagcggacc tcctggtcgg
       61 atacgaagtc ggagcgcgtc agcgtcgcga cgccgccgct cgcctcggtg atgccgcggt
      121 cggaaggttg tgtgctcata ccagctccag tagtcgctct tcgaggtcgc ctgcacggaa
      181 cggcaggccc ttcacctggt tgatcgagat cgtgtcgatg aggaagtccg cgcggatgag
      241 cctcgacagc tgccccatgt tcatctccgg gatcacgacc gtctcgaagc ggcgcaggac
      301 ttcctcggtg ttcttgggca tggggttcag gtgcttgagg tgggcccggg cgatcttctt
      361 gccgcgcaac cgcagccgcc tgcacgccgc gccgatcgcg ccgtaggtcg agccccagcc
      421 gagcacgagc accttcgcct cgtcgtcgcc gtcccactcg aggggctcga tgtcttcctc
      481 gatcgccttg atcttggcct ggcgcagcaa cgtcatgagc tggtggttct cggggtcgta
      541 ggagatgttg ccggtgccgt cggccttctc gatgccgccc acgcggtgct ccagcccggg
      601 ggtgccgggg atcgcccacg gccgggccag cgtgatctcg tcacggaggt acggccagaa
      661 gtcctcgccg gcgttgggct gcgacgcgaa cgggaccgag atgtcgggga gcttctcgac
      721 cgccgggatg cgccacggct cggcaccgtt cgcgagatag ccgtcggaca gcaggatcac
      781 cggcgtcatg tacttgagcg cgaggcgtgc cgactcgatc gccgcccaga agcacgacgg
      841 cggcgagtac gacgcgacga tcggcagcgg ggcctcgccg tggcggccgt acatcgcgtg
      901 gaggaggtcg gactgctcgg tcttcgtcgg gagcccggtc gaaggccccc cgcgctggat
      961 gtcgatcacg atcagcggga tctccaacga gagcgcgagc ccgagcgcct cggacttgag
     1021 gtcgaggccc ggcccggacg tcgtcgtgac gccgaggtgg cctccgaacg acgcgccgat
     1081 ggccatcgag atcgccgcga tctcgtcctc ggcctggacc gtgcgtactc cgaagttctt
     1141 gtggcgggag agctcgtgga ggatgtcgga ggccggcgtg atcgggtacg aaccgaggaa
     1201 cagcggaagc ttcgcgtgca cgctcgccgc gatcagcccg aacgcgatcg cctggttgcc
     1261 ggtgatccgc cggtagcgcc ccggcgcgag ggtcgcgggc cggacctcgt aggcgacgag
     1321 ctcggccgtc tcgccgaacg cgtgcccggc cttcaacgcc gctacgttcg cctgcgcgat
     1381 ctgcggcttc ttggcgaact tcttctcgag ccacgcgacg gtcggctcga ggggacgcga
     1441 gtacatccag cagatgacgc cgagggcgag catgttcttg ctgcgctcgg cctccttctt
     1501 gctgacgccg gcctccttga cggcctcgac cgtcatcgag gtcatcggga tctggacgac
     1561 ctggtagccc tcgaggctgc cgtcggtgag ggggttggcg gagtagccga ccttgtccag
     1621 ggcccggtcc tcgaacgcgt ccgagttcac gatcagcagc ccgccgcggg ttaggtcctt
     1681 gaggttcgcc ttgagggcgg ccgggttcat cgcgacgagc acgcccggct ggtcgccggg
     1741 ggtcaggatg tcgtggtcgg agaagtggat ctggaaggcg gagacgccgg cgatcgtgcc
     1801 cgcgggggcc cggatctcgg ccgggaagtc cggcatcgtc gcgaggtcgt ttccgaagga
     1861 cgcgctgacg ctcgtgaagc ggtcgccggt gagttgcatc ccgtccccgg agtcgcccgc
     1921 gaagcggatc acgacccggt ccacctcacg gcgctcgccg gctttgggcg gcgccacctg
     1981 ggagctcatc gattctcctc tgggttcaca actggcagtg aatggagaac cagtctaagt
     2041 cacccgacta ttacgaaggg aagaccttga aagcctcgat cgacgcgatc gtgacctcgc
     2101 gcgcgttgcc gcggcggtcc atgaccgtta tgtgcccgtt tgcgtcgacg gacatgagca
     2161 cgccgacgag ctcgctgctc gagaaacccg gctcgtcgga cagcttgtag cgcagcgaga
     2221 ccttgcgacc gaggagcttg cgcagctcag ctgggccggt gcggggcggc tctactcggt
     2281 gcgctcctcc gaaggagcga acgggctctc catcgcaccg cccgaggcgt cggggttgtc
     2341 ctgaaaccac tgcgcgttga cctgcgtgaa gttcgcccac tcggccggga tgttgtccgc
     2401 cgggaagatc gcgtcgacgg ggcacgctgg caggcaggcg tcgcagtcga cgcactcctc
     2461 ggggtggatg tagagctgct cctcgccctc gtagatgcag tcgaccgggc actcgtccac
     2521 gcacgcccgg tccttgacgc cgatgcacgg ctcagcgatg acgtacgtca tggtccggtt
     2581 ccctcctagg ggatggttga gcttctagcg cccgatgata cccacggggc agcgccggaa
     2641 aaggccgttt accaggaaaa acattgtgcc cttgtgtagc cggggctacg gcggcgaggg
     2701 cgagggggtc gacacgatcg gcatggcgaa gttgccctcc acgatgttca cgaacacgat
     2761 cgaggcgtcg tcgcacaacg tcctgcggat ctggatcgca cccaggcgct tcccctgctt
     2821 caggtagacc tcggcctcga acccctcgtt gtcctcgctc actatgtcga agcccgcggc
     2881 gcgtaccgcc tttcgataca cgggcagggc ctcctggacc cccagctcga cgctcaggac
     2941 tccggtcacg cctcccttgc ggcggccggc cgacgcgacg ccggcgtcac cgtcgaggag
     3001 gaagggctcg gggatcagct cggggcgggc ggccgggtcc gggctcggga cgccacagtc
     3061 cgccggcacc gcaggcgcgc cgcccccgcg gttgcacgag acgccggcga ggagcaagag
     3121 cgcgatcgcc acgagggggc cgcggtcagt cctcactgat cgtcgtcgcg aggaacgcgt
     3181 ccacccccat ccgccgcgcg accttcatcg agacgtccgg cgacagcgcg tagaacacgt
     3241 ccgcgacctt cgccagcccg cgcgcgacga cgacctcggc cctgttcttc tcgatcgccc
     3301 ggagggccgc ggccgagacc tccggcggcg tgaccgtcgc gatcgagcgc ggcggcttcg
     3361 cgttgttgct gcggtccgcg tacatcccga tgtcgctgac gaagctcggg cagaggaccg
     3421 agacgcccac gccgtgctgc gccatctccg cacggagcga ccacgagaag ccgacgagcg
     3481 cgtgcttggt cgcgccgtat accccgtagt acgggacgcc gctcttgccg gacgccgacg
     3541 acacgttcag cacgtgcccg cggcggcgct cgatcatccc gggcaccacg aggcgcgtga
     3601 gcttcatcac cgcggtcagg ttcgtctcga cgatcgcgtc gatccggtcc atgtcgaacg
     3661 tcgcgaacgg agcgatggtc tcgatccccg cgttgttgac caggaggtcg ggggggccga
     3721 gctcgtccgt cgtacgtgcg accagcgtct cgaggtccgc gaccttcgtg acgtcggtcg
     3781 ggacgacgat cgtgcggacg ccggagcctt cgagctgccc ggcggtcttg cgcaagccct
     3841 cctcgtcgcg ggccgcgagc gcgaggtgga ctcccctgga ggcgagggcg cggccgagct
     3901 cgacgccgag gccgcgcgac gcgccggtga ggatgcccgt cctgcccctc agctccacct
     3961 tcgctccctc caccggcggt agatccagta ctgcagcgcc aggatcgcag ccgtgaagac
     4021 gaacatgacc acgctgaccg cggacgcgta cccgaaccgg aagtagcgga agccggcctc
     4081 gtacacgtac agcggaagga acgtcgtcgc gtaggcgggc cccccttccg tgacgaggaa
     4141 ggccgggacg aagttgacct gtagcgcgag caccacgtcg cgcaacgcga gcaacccgag
     4201 gacgggcgcc atcaccggca gcgtcaggtt ccgcaacgtg aaccagggcc gcgcgccctc
     4261 cacccgcgcc gcctcgtaga gcggctccgg caccgtccga cgcgcggcca gcgcgacgac
     4321 gaacgcctcc ccgatctgga acgcgcccat caccgcgatc gagacccgcg ccgcccacgg
     4381 ctcggtcagc cagcccggag ccgtcagccc gagcgacgag gcccccgcgg cgatcggccc
     4441 gtacagcggg ttcagcaacc acagccacag cagcgcgtac gcgacgtcgg gcacgacggt
     4501 cgggaggtac gcgaccgcac gagctgcgcc cgatcccgcc gtcctgcgat gcaacagcag
     4561 cgccagcgcc accgccgccg cgatccgtag cgggacggcg atcgcgacga acgcggcggt
     4621 gttgccgacg gacttccaga acagcggctg gtcccagagc cgttcgaagt tgtcgaaccc
     4681 gatccactcc gggtcctgca ctccggagaa gtccgtgaac gcgagggcaa tggcccccgc
     4741 gacggggatg ccgacgagca gcgtcaggcc cgcgacgtac ggcagcgcga agcgccccgt
     4801 ccgcatcaca gcgcggcgcc gtccctgccc gagaagcgcc ggatcctgct ctcgtcgaac
     4861 gagatgccgg tctcggcgcc gaggcgcggc gagaaccctc tcgggacctt gacgagtacc
     4921 cacgtcggac cgcagtcgac gtggacgacg gactcgttcc cggtgaactc gacctcggct
     4981 atgcgcccgt cgaggcggcc ggcgccgggc gggacgagtg cgacgtgctc cggccggacg
     5041 ccgatcgtca cgtccgggtc ccacggcggt gcgtcaatca ggttcatcgg cgggttgccg
     5101 acgaaccgtg cgacgaacgc ggtcgccggt tggtcgtaga gctcgacggg tggagccacc
     5161 tgctcgacga gcccgtcgcg cagcaccgcg acgcgatcgg ccatcgtcat cgcctcgacc
     5221 tggtcgtgcg tgacgtagag catcgtcgcc ccgaccgagc gctggaggtt gcggatctcg
     5281 gcccgcatct gggcccgcag cttcgcgtcg aggttcgaga gaggctcgtc catcaggtag
     5341 gcgtcgggct ctcgcaccat cgcccgcgcg agcgcgacgc gctggcgctc gccgccggac
     5401 agctcgttcg ggcgccgatc gaggacgtct tcgaggtcga ggtgccgggc ggcagactcg
     5461 agcttgcgct cgatctcgcc gcggccctcc ttgcgcgccc gcataccgaa cgagatgttc
     5521 gcggcgacgg tcatgtgcgg gtacagcgcg tactcctgga agaccattgc gacgccgcga
     5581 tcgccgggcg gcgacttcgt cacgtcgcgg cccgctacct ggaccgtgcc ctcgtccagc
     5641 tgctcgagac cggcgatcga ccgcagcaac gtcgtcttgc cggaccccga ggggccgacg
     5701 accacgagca gctctccgtc ggccgcggtg aggtcgacgt cctcgagcgc gacgacggac
     5761 ccggcgcgcg agcgataggt cttcgtcgcg ccggcgaccg cgatgctcac tcttggagcg
     5821 cctcctcgaa gaggccccgg gtctccgtgt cgaggtcgct tatcgcttcg tcgacggagt
     5881 agccgtcctc gtagaacgcc cggatcagga tctgctcggc gacgtcttcg atctccggcc
     5941 acgtcgacag caccggcgtc cggcggatcg tcgggatcgc gtccaggaag accttgctgt
     6001 gcgccggggg ctggaccggg tcgaggaacg cgccggactc cgcgacggcg ctgagcgacg
     6061 ggaccgtgcg gccgctcagg gctccgagcg tctgcccctc ctcgccggta gcgaactcga
     6121 cgaagtcggc cgcgacgtcg agctggtcgg agcccgccgc catgcagaag ccgtcggaat
     6181 gcaggatccc ggcgggctcc tggtcgaccg ggagcggcgc cacgtcgaac tcgagaccct
     6241 gcacctcgcg gaacgcgggg acctctcgcc gcgacgagag cagcatcccg agcttgccgg
     6301 tcgtgaagcg ggtatcgagg tcctgcgcgg cgacctcctc ttcggtcggg atcagcccgt
     6361 ccttcaccat cgagacgaag aagccgagcg cggtgcgcgc gccgtcgttg tcgaggtcga
     6421 gccgggtcgg gttgtccggg tcgtcgacga tgtcgccgcc ggccgaccac gcgaacggcg
     6481 cgagccggat gatcgtgggc tcgatgccgg cgccgcggac gtcccccttc gtcagggtcg
     6541 cagcggtgtc gcggaagtcc tcccacgtcc acccggcctt cggccgcttc agcccggccg
     6601 tctcgaacag cgcggtgttg tagtagacga cgagcgacga gatgttctgc gggaagcact
     6661 gcagcgcgcc gtcgtacgtg aaggcttcga gcggcggctc gtagtacgcg ccgaggtcga
     6721 cgccgtcgag gtggttctcg atcggctcga tcgcgtcgcg cacgacgaac tgcgagtact
     6781 cacggaagtt cacgaggaag acgtccggcg ggttcccgcc ggcgaagctc gtcgcgagcc
     6841 gggccagatg ctcgtccttc tcggggatct cgacgacctc gacgtgcgtc ccgtcgttcg
     6901 cctccatgaa ctgctcggcc atcgacgagt agaccgcggt ctcctcgggc tcgcccgaga
     6961 cctggagctc gatgtgggcc acgccgccct cgtcttcctc cccggccgcg tcgggcgagt
     7021 cggagcacgc cgcggtcacg agcgtcagga ggagagcgag ggcgacgagc ttcctgctca
     7081 tgcggggcct cctcagggtc cgacggtctt ggtcaggaac gtcctctgag cgaggaagaa
     7141 cgcgaccacg ccgggaagcg taacgaccac cgacgcagcc agcaggatcg ggtgcagcgt
     7201 gggctcgagg gcatgcaagg accgcagtcc gagcgggacc gtgtagaggc tctgctccga
     7261 caggtacagg agcggctcga cgaagctcga ccaatggaag acgaacgcga gcatcgcgac
     7321 ggcgaacgtc gcaggccgtg cgagcggcca gccgatcttg cgccacgtcg cgagcgggga
     7381 gagacgctcg agggcggcgg cctcgaagag ctcgcgcggg atccgtgcgt agacgagcgc
     7441 gaacagcagc acgtaaaagg gcgaggtcgc catcagcgcg gggagcatca gcggaacgaa
     7501 cgtgtcgagg atccctgccc actcgaacat cacgaagcgc gggacccaca gcgagctcac
     7561 gggaatcatc aggcagacga gcgacagtcc gatcagcagg cggcgcgtcc ggcggccggc
     7621 cgtcgctatc gcgaaccccg cccaggacgc caccaagacc gtgaccggca ccgccaccgc
     7681 gacgacgaac gccgagttgc ggacgtaggt ccacatcggc acgaacagcg tgacgtcgcg
     7741 gtagttgccg gtctggacga cctcgggcac gaactcgaag ccgtccggtg gagggagccc
     7801 cggccggcgc aacgagcctg cgaccatgaa cgcgagcggc gcgaggaaca gcgccgagac
     7861 ggcgacgatg gcgatcctgc gcgcccatct gtgtccgggc cgctcaccga cgcgtccggt
     7921 cgtacgtctg ggccgctcgc cgaggcgacc ggtcgcgcgg ctcatctgcg gcgctcctcg
     7981 tagaggatgt ggagcgtcac gtcgtcgcga caaggacctt cccgcagcac gtacctcccc
     8041 gcccttccgt cgcggagcgc gaaatatatc tccgcctcga agccctcgtt gtcgccactg
     8101 gtgatctcgt gaccgccttc gaccaggccg cgcgcgagct gcggatagag ctcgacgacg
     8161 gtctcctgtg tgatcacggt cacggcgctg aagccgcgcc ggcgcccgac ctcgacgacg
     8221 tcaccgcgat gcgtctggtc gagcaccgcc gggacgtcac gcgggggctc gacggcgggg
     8281 cgggagggac acggcgtggg ccgcgccgcg ggatcgcccc cgccgcaccc ggaaaggaaa
     8341 agggggaccg cgacgagcgc gatccccctt cgatacatgg cccgagacta cgaccgtcgc
     8401 cgcaggcgac gcagccgcgc gccgtggacg ttcccatcgg acggggccgc tcgcggcccc
     8461 gttaggggaa acggtatagg tacaccccac ggccgaacgt ggccgccact atcagcttct
     8521 tgtcctgcgg cgcctgctgg agctcgttca ccggcactcg gaccaggccg cgcatcgccg
     8581 gggcccacgc gctcccccgc ttgtccttcg acaggaacgc cccgatctgc gtcccgatca
     8641 tcagctgctt accgcggacg atgatcgtga acgccggggc cttcggcagc ccgcgcgaga
     8701 tgctgcggaa ggtccggccc ccgttggtcg accggtagac gttgccgcgt cccagctgct
     8761 tgttctcgtc gccgtaggag cccggcgggc gccactgccg gttcgcgtag ccgccgagcg
     8821 tgacgtagac ggtcttcggg ttcttcgggt cgatcgcgag gtcggtgatg tagcggttcg
     8881 cgaggccctt ggccggcgcg atgtgccact tgccgccgac gttcgtggct atcccgttct
     8941 tgaacggctc cgcgctcgag atgatgtcgc agacgccgca gaagccggcg taggcggtct
     9001 tgccgcgcag cgcgacggcg ctcatctgcc ggggtacgtc gttcttctcg gtgccgagct
     9061 cgaagacgtt gacccagtcg ccctccgggc cctccttgcg ctcgtagagc gcgtacccgc
     9121 cggtcatcag gtgcttcgcg tcgagcgggt ccatcacgaa ggggttgccg aacatgtggc
     9181 cggagtcccc cgtcccgatg tcggtccagg tctggccgcc gtcggtcgtg acgcgcaggc
     9241 cgccgccctg cgactcggtg tacgcgacgt cgctgttgtc ggggtcgacc gcggcgtaga
     9301 agccgtcggc gccgtacgtc atgtactgct tctggtccgg ctcgatcttg cccgagccgt
     9361 tgtcctgcat cccgtaccag acggtcccgt ccttggccat cgccgctccg tagggaagca
     9421 gcgtccggaa gccggtgttc gccccgtcgc cccagccggc ctggttgagc tcgaggtcct
     9481 cctggatcgt ctggacgtag gcgccaccgt cgttgccgat gaccaggctc aggtcgcctt
     9541 cgccgtggct gacccagatc gcgtcgtgct ggtcggggtg gacggtcgtg atcccgctcg
     9601 ggttctccgg cggacacgtc gggatgccgc cgacgccgat gatgcaggtc tccgccgcgt
     9661 agtaccggcc gatgaccttg aagtcggacg gcccgatcag cggctggcgc gtcacggtgt
     9721 tctgccacac ctcttcgaga ccgaacgtga gccgcgtggg gatcccgttg acctggcgcg
     9781 tgggatcggg cgcgatccac tcgttgtacc aagcctggat gcccggcccg ttgaagaggg
     9841 cctggccgac gccgacgagg gccgagcccg tcgcggggtt ctccgcgatc gtctcggtgt
     9901 tcgccatctg cgtccacgtc tgcccgaagt cggcggtgac gtagatgccg ttgaagttcg
     9961 tgttcggcgg caacggcgac tcgccgccct cctcggggac gtcgatcgac gacggcggcg
    10021 cgccgttgta gagctcggcg tcctgcacga tcgcgtagac gtagtcgtgg ttctgctccg
    10081 gcccgacggc ggcgccgagc tcgatccggc cgatccggtg ctgcggagtg aagccggggg
    10141 cttcgagctt gtcgaacgag cccggcttgc cgttcttcga ccagtagagg ccgttgttgg
    10201 gggactcgag cgtgtcgggg tcctgcggga acggacgtgc gccggcgcgg tagccgactg
    10261 cggcgaggac ctcgcctccc ttgttctcga agttgtccgg tgcctgcacg acgacgtcgg
    10321 tcacgaagtt cgcgagcagg cacttgccgg tgtgcttccc cttgcagggc ccggtcggga
    10381 gcttgacgtt gcggtagcta cgcccggcgt tggtcgaccg gtagaggccc ttgctcgtcg
    10441 cgacgtagac gatgttctgc ttcgcctggt cgatcgcgac cttgaacgcc atcgccccgt
    10501 tcgggacgcc cttcgcgcgt ctccacgtct tgccgaggtt ggacgtgtag aaggcgccga
    10561 ggcccgtgta agtgttcccg cccatgagcg gctcgccgct cccggcgatg agggtcccgc
    10621 ccttggcctt cgaccagccg atcgcaccga cgatctgcgt cggcagcttg tcgccgatcg
    10681 acttccagct ctcgccgagg ttgcgtgaca tccagacccc gccggtcccg accgacgcga
    10741 acaaccggtt ggtcctgggg tcgtggccga ggctgtcgat gcggccctgc aggtgcacga
    10801 agcccgcgcc gttgacctcg tcgaagcgcg ggtcgttcgc gatgaggggg cccttgccgt
    10861 acggagacca gcggccgcgg gtgccgggga cgcgcgcggc cctcgctgcg agcgcatccg
    10921 ccctcctcac cgctttcgcg agagcgttcg ccggcacgcg gccgtgcggt gccccgtgga
    10981 tcgtcgcccg catgccctcg gccagcgcca tctcggcgac cggctcgggg accttcggcg
    11041 gggtcgagca gacgacgcct ccgatctcgg cggcctctcc ggtcatctcg cggagctcgt
    11101 ctccgacggg gacggcgcgg ctaccgggag gacattgcag ggccgcggca ggggtaccag
    11161 ggccgggact ggcgagcgcg ccgacggtta tcgacgcgca gacgacgaga acgaggaccc
    11221 ccgcaagggc cctcgtggag agagacttca cctgcaccac ccacctcaac taggacgtga
    11281 cggcacccta ggccatcgcc gcgcctgccc tacccgttat ccggggcctg gcaggaactg
    11341 cgaagtacct cgcaggcacc ggcgctggcg ggattcactg gcgaccccgc gaggagacgc
    11401 gaaatatcca aatcgccgcg acgagaccag cccgtcgaga gggatcacag gtccttgggg
    11461 ccggagaggc gcttgagcga ggccccggca tctctgcgtc ggcgagctac acagcgccct
    11521 ctgagctagg accctctata tcggggccat cgtccagctg cgcgagcgtc tcatgcttcg
    11581 tcgggtcgac cgtctcgtag ttgaacgaat atcgattcat agccgccgcg ctggtcaccc
    11641 aacgcttcac gccgtctacg agatagacct tcttgtcgct ttcgtcttgc gcgaggtggg
    11701 ccccggtgct cagctgatct ccgggggcta tgcaaatcgc gtcgatgtcg ctgattatgg
    11761 gggtacccgg acggaacagc gcatgcaaca cgtcattcga cggtacccac cggcggacgc
    11821 cgtccaagtc gaccaggtac acgcggtcgt cgccgtatgc cgggctcttg accagaagcc
    11881 caacgaggtc aggccgtttg gtagtagcaa ccatctctcc acccccgtgg tattactcct
    11941 aacttaacgt taggttatcc agaggtgagg gcgccgcgct agtaggtcgc ggagtttccc
    12001 gtcacggttg gtcgaagagc acccaggcgt tcggcggccc gcgcgtattg cttgcccgcg
    12061 tggtagctgc tgcggaccag ggggccgctc tcgacccacg cgaagtccag ctcgtcttcg
    12121 ccgaacgcct tgtagcgcgc gaactcagcc ggctctaccc agcggtccac cgggaggtgc
    12181 cacgacacgc tcggctgtag gtactggccg atcgtcagca cgtcgcagcc cgtcgcgcgc
    12241 aggtcccgca tcgactcgta gacctcttcc tcgcgctcgc ccatgcccac gatcagattg
    12301 ctcttcgtca cctggtccgg ccggatctcc ttcgcccagc gcagcaggtc cagcgtcgcc
    12361 tcgtagccga agcccggacg gatcgccggg tgcaagcgcc ggaccgtctc caggttgtgc
    12421 gccagcacgt cgggccgcgc ctcgatcacc cgctccagcg aagcgcgcgg cgactcacga
    12481 cgccccttga agtccgggat caggatctct atcccgcagt ccggcatcgc cgcgcggatc
    12541 gcacgcaccg tcgccgccca gtgagcggac gccgcgtcgt cgaggtcgtc gcgagccact
    12601 cccgtcacga ccgcgaaccg cagtcccatg agccggaccg cctccgcgat cttcgccggc
    12661 tcttcggggt ccaacgcaga cggcttcgca gtcatcacgt cgcagaagct gcagcgccga
    12721 gtgcacaggt ccccgcacag caggaacgtc gcctcccgtg cctcccagca ttcgtagatg
    12781 ttggggcacg ccgcttcctc gcagaccgtg tgcagccccc gctcgcgcat gatcgagcgc
    12841 agctccgaga agttctcgcc ggtcttgacc cggaccttga gccactccgg ccgtcgctgc
    12901 ggccgcggcg cttccggccc cggcgcggcg tgcaacggag tgccgcgacg gaggtcgcgc
    12961 tcgatcgact cgcgcgtccg gatcgtgggg atgacggtgc gaggcgccaa gctagaactc
    13021 cgccagcttc cgcatctcct gctcgatctt gtcgacgtcg ggcatgaacc acgcctcgag
    13081 cggcggggcg aacggcatcg ccgggacgtg cggccctccg atgcggacga tcggcccgtc
    13141 gagctcgaac atcgccttct cggcgatctg cgcggcgacc tcggccccca cgcccaggaa
    13201 cttgttgtcc tcgtagacga ccatcgcctt gctggtcttg ccgacggact cgaggacggt
    13261 gtccatgtcg agcgggaaga tcgaccggag gtcgatgacc tcgacctcga gcccctcgcc
    13321 cgacagccgc tcggcggcct cgatgcagta gtggtgcatg agcccgtacg acacgcacgt
    13381 aaggtccgtg ccctcgcgtc gtacgacggc cttgccgagc gggatcgtgt agcgctcgtc
    13441 gggcacgtcg cccttgatga ggcgatacgt cttcttgtgc tcgaggtaga tgaccgggtc
    13501 ttcgtcctcg atggcctgga tcagcatccc cttcgcgtcg tacggagtcg acgggacgac
    13561 gaccttgagc cccggaacgt gcgcgtagaa cgcctcgacc gactgcgagt ggtacagcgc
    13621 cccgtgcacg ccgccgccgt agggagcgcg gacgaccatc ggcaccgaga agtcgccgtt
    13681 cgagcggtac cggatgcgcg cggcctcctg gatgatctgg ttgaagcccg ggaagctgaa
    13741 gtcggcgaac tgcatctcgg cgaccgggcg cagcccgtcg atggcgaggc cgatgccgac
    13801 gccggcgatc agggcctcgt cgagcggcgt gtcgatcacg cggccctcgc cgaactcgtc
    13861 gaggaatccc atcgtcacgc ggaagacacc gccgcgcgcg ccgacgtctt caccgaggac
    13921 caccacgcgg tcgtcccggc gcatctcctc ctgcagcgtg tcgtggatcg cggtgactac
    13981 gttcttctcg ggcatggcgt cacaccacct ccagatccgc gtacacgttc gtccctgcgc
    14041 tcagcgggtc cgggaagtcg gactgctccg cctccttggc gccctcggcg acctcggcct
    14101 tcgtcttctc gcgtagctcg gcgatcctgc cgtcgtcgat catctcgacc gacttgagat
    14161 aggcctcgaa gcgaggtatc ggatccttgc gcttccactc ctcgacctct tccttctttc
    14221 gatacgtgcg gtcgtcgtcg tcggaggtgt gcgagtagtg gcggtaggtg cgcatctcga
    14281 tcagagtcgg gccctcgccg gcgcgggcgc ggtcgacggc ctccttcgac agcttgtagc
    14341 actcgagcac gtcgttgccg tcgacctcga agcccgggaa gccgtagccc tcggccctct
    14401 tgtagacgcg gccgccgagc tgcttcgact cgtgcaccga gatcgcgtac tggttgttct
    14461 cgacgacgaa cacgagcggg agcttgtgga cgccggcgaa gttcatcgcg ccgtgccagt
    14521 cgccctcgga cgtcccgccc tcaccgcacc aggtgccgac gaccacgtct tccttcttca
    14581 gcttcttcga gtaggcgata cccgcggcgt gcaggcactg cgtcgcgatc ggcgaggagc
    14641 ccgtgatgat gttgcgctcg cgactgcccc agtgcgccgg catctgacgg ccgccggagg
    14701 aagggtcgtc cttcttgccg aagaggccga ggaagacgtc cttcggcgtg aagccggcga
    14761 cgagcacgag cgccatgtcg cggtagtagg ggcagaagac gtccttgccg cgctcgaacg
    14821 cccatcccga gcccacctgc gcggcctcgt gcccggatac cggcacgacg aagggggcct
    14881 tgccctgacg gttgagtccc cactggcgct cgtcgcagaa ccgcgccatg agcatgtagc
    14941 ggtacatctc gagcaggtct tcctccttca gcccgagatc ggtgtgcagg tcctttcctt
    15001 tcgccatggg tgctccttgt ctacagggcg ccggccgtca ggtgcccggc gcggtgcagc
    15061 gatcgtggat gggtccatac gagagtgcgg tcgaaggtct cggagaggta cagggccgcg
    15121 agcgacgcga cgtccttgag aggccggatc ttcccggtct ccgctgcgac cgacgtgacc
    15181 caccggtcgc ggatgccgca cggcacgatc ccctcgaaca tcgagaggtc ggtcgcgacg
    15241 ttgaacgcga acccgtgcat gctgatcccg cgcgtgatct tgacgccgat ggcgccgatc
    15301 ttgttgcggc cgacccatac gccggtgtgg tcgtcgtcgc ggtgtgcctc gacgcccaga
    15361 gcgccggcga ccttgatcag gacgtcctcc agcttgcgga gatacgagat cacgtcgtag
    15421 cgctcgccca gcgagaggat cgggtagccg acgagctggc cggggccgtg gtacgtcgcg
    15481 cggcccccgc gatcgacctc gtacatcgag atcccgcgcg tcgcccgttc ccggtcgccg
    15541 tagacgaggt cttccttcgt cccgcggcgc ccgagcgtgt acgtcggcgg gtgctccagc
    15601 agcatcaggc tgtcgccgat ctcgccgtcg agccgcgcga ggaacagctc ctgctgccac
    15661 gaccaggccg cggggtactc gacccggccc agccaggatg cggtcagcgc ggtcggcggc
    15721 gtcttgctca caggtacggg gacagctccg gcgcgaagtc cgtcgtctcg atccgctcgc
    15781 ggagccgcgc gaggaacttc gcagcctccg cgccgtcgat cgtccggtgg tcccacgaca
    15841 tcggcaagaa gcccatggac ctgatggcga tggcgtcgtc gtcggtgacg accacgcgct
    15901 tctggatcgc ctcgaacgcg aggatcgcgg tctgccccct cgggatgatc gggaccgaca
    15961 tgatcgagcc gaacgggccg gggttcgtga tcgtgaacgt cccgcctgag aggtcgtccg
    16021 gcgtgagctt cttgctccgg gccctcgtcg ctacgtcgtt gatcgcgcgc gcgatgccgg
    16081 cgaggttctt ctcgtcggca cccttgacga cggggacgat gaggccgccg ccgtcgagcg
    16141 ccaccgcgat gccgaggttc acgtagcgct tcagcacgtt cgcctgctgc tcctcgtcga
    16201 aggtcgcgtt gacctcgggc atctcgagga gcgtgtcgca caccgcccgg gcgaggaacg
    16261 gcagataggt gaggttgaag ccctcgagct cgcggaagcg ctccttcgcc ttcgtccgca
    16321 gggcgacgac gcgggtccag tcggcctcga ctgcgttcca cgcgcgcgcc gcctcgtgcg
    16381 acgcggcgac catgtgctcg gccgtgcgcc ggcggatgtt cgaccacggg acgacctctt
    16441 cgcgcgcgcc gccggacgct gcgggagcct cttcgcggcg cgggggcggg ggtggcgggg
    16501 cgtcgcgtcg cgccggctct tcctgtcgcg gcgcgggctc ggcctgctcg gacggacgcg
    16561 cctcgcggcg agccttgccc tcctgcacgt atttgaggac gtcctgcttg cggatgcggc
    16621 caccggtgcc ggtcccctcg atctcgtcga ggtcgacgtc gtgctcgtcg gcgagcctgc
    16681 ggacgagcgg cgagatgatc ccgcgcttgc tcgagtcacc cggtcgtgag gagggtcctc
    16741 ctgtggacgg acggctcgac ggagcgaggt ccttcttctc ctgcttgcgc gtcgccgacg
    16801 cgtcggcctt ctcggactcc atccgttcct gcgacccgcc cgagccctga gtcccttggt
    16861 cccccgcggc ccccgactcc tgtcgctgct gcgcgacctg ctcggcgcgg tcccctacct
    16921 cttcggacgc gccttcgtca ccttcgggga cgcccgcgct gctcgcggct tgcgtctcgg
    16981 cctggtcggc ttccgcggca tcgccgccac cgccctcgac ggcgccttcg ccgacgatcg
    17041 caagcgcggt ccctaccttc accgtgtcgc cctcttgtac gagaatcttc tggatcgtgc
    17101 ccgacgcgga cgacggcacc tcggagtcga ccttgtcggt cgagatctct acgaggagct
    17161 cgtcctcttc gatcgtgtcg ccctcttgct tgagccaccg ggtgatcgtc ccctcgacga
    17221 cggtctcgcc gagctgcggc atcttgatct cggttgccat gtaaggccga cccctccctg
    17281 gtttagagaa cccctcggtc cgcgggcgct tgcgcgctcg ttcgaggacc ggtgtgtgtg
    17341 ctccccaggg tacccgcgcc cccgccccct ctgctcggga gccgtgagtt gggaagctcc
    17401 tcgaacttcg gcgggggcct cccgtcgacg tcacgcagga gctcgaggta tcgagccggt
    17461 cctggctgaa ctggccgggc ccggtgaacc tcgccggtta gcccgtcggg gcctcgatct
    17521 cctcgtagtc cgccggcggc tcgatgtcga cctcggcgcc gtagtccgag aagcccatcg
    17581 ttatgagcat ctcgatggac tggccctgcg cctccatcga catgtcgagc ttcatctggc
    17641 gcgggaggcc ctcttcgtcg atccacacgt cggcaggtat cgagtcgacc tcgccgccga
    17701 actgctcgag ctgggcgcgg atctcgtccg cggcctcctc gtcgggggcc tggtcgagga
    17761 tcttctcgaa cgacagctcg gccttgtagt gggtcgtctc gtcgccgtcg atcttctcgg
    17821 tcccgacctc ttcgagaccg tcctcgctcg ccccgcgcag gaactcgagg tactggctcg
    17881 ggtcctggct catctgcggg ttggtcgccg cacccgggat cgccgacgcg tccatccgga
    17941 cccagtcacc ggtcccgccg agctccggcg gaagctccat gtagatgtag ttgccctcca
    18001 tcaccatctt gatggtctgt tctccggggg tgccgggcac cgccatcgtc atcgtcatct
    18061 cggcgagctc gttggcgaag tcgagctcac cctcggcgtc gacggagacg gtctggccgg
    18121 cggcgctcat ctccatcgtc atcgtcatcg tggcagtgcc ggcctcggtg gccttgtcgg
    18181 ccgcgagcgc gagcgcgtcg accgagtcgc cgccgccgcc cccaccttcc tcaccgcacg
    18241 ccacgccggt gatcgcgaga gccatcgcga atagccccgc tgcaagcttc ctcatcgtcg
    18301 tccgcctttc gtcgtccgtg cctggagcat cgccgaggcg atgcttcgtg ggcttagccg
    18361 tggagcggct tgcccgccag atccaggaaa gtctcgccga ccacctcggt aagggtcggg
    18421 tgcgggtgga ttatctccgc gagctccgac ggatatgcct cccacgacgt gaccagcatc
    18481 gcctccgcga tcaggtccga cgcccggggg cccacgatgt ggacgcccac gacggccccg
    18541 cccttctcgg ccacggtctt cacgaagccc tgcccaccgc cgagcatgag ggcccgggcg
    18601 ttgcccgaga aggggtagcg cttgatctcg acgtccatgc cgagctcctt tgccttgtcc
    18661 tcggtcaggc cgacggcagc gacctcgggc tggcaataga tgacgtgcgg gacgttgcgg
    18721 tagtcgacgg cggtcgggct ctcgccggcg aggagctcgg cgacgcgcat gccctccgcg
    18781 taggcgacgt gggcgagctg cgggtggggc cagttcgcgt cggggaccca gatcgcgtcg
    18841 ccgatcgcga agacgccgtc gacgctcgtg cggaactgct ggtcgacgac gatgaagccc
    18901 ttgtcggtct tgatgccggc ctcttcgaag cccatgccct cggtgttcgg gccgcggccg
    18961 atcgcgacga acatgcggtc cgcggccagc gtcttgcgct cgccgttctt ctcgatctcg
    19021 acctcgacgc ctgagtcggt cttggtcgcg cccgctaccg aagccccgag gtggaacttg
    19081 atcccgcgct tctgcaagag cttgtggagg gcctcggaga tgtcggcgtc ctcggccggc
    19141 gcgatcttcg ggagcatctc gacgacctgg acctcggcgc cgaaggagcg gtagacgctc
    19201 gcgaactcga gcccgatgta gttgccgccg acgaccacga ccgagctcgg gatgtcctgc
    19261 gccgagagcc cgtgcgtcga gttctggacc ttgtcgccgt ccgcggcgat gaacgggagg
    19321 tcacggggga acgagcccgt cgcgaggacg acgcccttgt tcgccgtgag cttccgatcg
    19381 ccgtccttcg tcgtgaccgt gaccgtcttc gcgtcggtga gacggccctc gccctcgacg
    19441 agctcgaggt tcttcttgcg agagaccaag ccgctcagcc ccttgtacat gcggccgacg
    19501 accgagtcct tgaagttgag gaccttgccc cagtcgacct gaggctcctg gacgatcacg
    19561 ccgaagttgc cggcttcgcg gatctcgtcg gtcagctcgc cggcgtgcag taaggccttg
    19621 gtcgggatgc agccgcggtg caggcaggtc cccccgacct tgtccttctc gaccatcgcc
    19681 accgtcaggc cgagggcctc ggcgcggaag gctgcggcgt aacccgcgtt gcccccgccg
    19741 aggatgacta cgtcgaaggc gttgttcccg ttcgccactg cgctacctcc acgtcgagat
    19801 ctttgtccgg ccaggataag cggcggggcg gagggctcag gaggaacatc tcgccggggc
    19861 tcgaatagct cgcttacatc ggtctagggc ggaggtatag ccatgaggaa aatgcgggtc
    19921 ctcgtcgcga cgatgggtgc ggcgctcgcg atgacgctgt tcccggcatc ggcgcaggcg
    19981 gcgcaccact gtgcgtaccc gccgatcgac gacaccgagg gcgtcgtcta catggtgtgg
    20041 ctgacgtgcg agtacggcta ccacgacccg gtgggcgtgg cgctgtacgc ggcgtgctgg
    20101 ctgtcgccga cctgcccgta gagacctttc tctatagagc tcaggcacct ggcgggatcg
    20161 tcgaggtccc gccaggtgac cgtccacacc tgccacccgc ggtccaccag gaagttgtgc
    20221 ttggcgcggt cgcgctcgaa gtcgggggca gaggaatgcc agcggcggcc gtcggcctcg
    20281 agcccgaccc gccggtccgg ccaggcgaag tcgagccggt agggcccgac ctcgtgttcg
    20341 cggacgaacg gcgggagccg ggagccgcgg aggatggcct cgacccgccg ctcgagggcc
    20401 gaggacgttg cggggccgag ggcctggatg aggcggcggg agccgcgccg ccccgggttg
    20461 cgcccgacga cgtcccgaag ctgagcggga ctgacgatcc cgtcgatcac ggaccgctcg
    20521 accagcgtta acaggctgtc gccgccgcat cgggacgcga cgtcgagaag ggtccgggcc
    20581 gggctcgtga cagcgacggc gcgcaacttc ccgcggtcac gcttcgccaa ggtcgcgacg
    20641 tgcgcctgga ccccgcacgg gggtgaagtc agcctgcgcg gtgccgtgac gtgcagctcc
    20701 ggtgcggctc ctgtcatccc ccacagcgcc gctgcagtct cgtgcgacgc cacggcatcg
    20761 ggaccggccc acagcagcgc cgccaccgca cgcagctccg gcgtgacgcg agcaccgccg
    20821 atcctgtaga cgccgggaaa gacgcgtcga agcgaaccgt cgcgcgccct cctggagacg
    20881 gcgctgcgcg aataacccaa cgcttccagc tgcctcagcg agaccgctcc gtgctgccgc
    20941 gcggccaacc cggcgaggtc tgctcgtcga gttccgtttc cgtcgcgcat agcgcgacag
    21001 gatcggatct cgccgacgaa caagtggtgg cgggggccac cgccgggggg gggttagctg
    21061 ccggcgcccc ggctccccag ctctcggtcg aatagcagga ggctcgccgt cgagtcgccc
    21121 accatcttca gggtctccac cagcgtgccg gaggtcttct cttcctcgac ctgctcctgg
    21181 atgaaccatt ggaggaagac ctggctcgcg tagtcaccct cgtcgagggc ctggcggtag
    21241 aggccgtcga tggcctcgga gacgtggcgc tcgtgctcgt gggcctgctg gaacgcgtcc
    21301 agcggcgact tgaacgtcgc cggcggcttg tcgagcgaca tcagctcgac gctcccgcct
    21361 cggtcgtaga tgtactcgta gaacttcatg ccgtgcgtcc gctcctcgtc tgcctgggcc
    21421 cgcatgcacg ccgccgaccc ggggaggttg ttcgattcga agttggagga catcgcgagg
    21481 tagaggtggg cggcgtagag atccttgctg aggtgctcgt tcatggcggc gcccaggctc
    21541 ttgctaagca tcggcgggtt cctttctcgt gtgtgaggtc ccgagcatcc accgaggtgc
    21601 ggcggcgcgc aatcgagggc gggcgaacgg ccggggtt
//
LOCUS       DASQDO010000217         6148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1123546, whole genome shotgun sequence.
ACCESSION   DASQDO010000217 DASQDO010000000
VERSION     DASQDO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123546"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..80)
                     /locus_tag="VEV43_06255"
     tRNA            complement(7..80)
                     /locus_tag="VEV43_06255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Arg,seq:acg)
     gene            complement(103..191)
                     /locus_tag="VEV43_06260"
     tRNA            complement(103..191)
                     /locus_tag="VEV43_06260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Ser,seq:gct)
     gene            234..1292
                     /locus_tag="VEV43_06265"
     CDS             234..1292
                     /locus_tag="VEV43_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYP23157.1"
                     /translation="MRRLLPLLTIVALAATALPGPTAGAVPKGTRVQLYRGDLDFPID
                     MAWVPGTKKVFFTEKNTGGIRVMAGRRLLARPCADLDVEPSGERGALGIVLHPKFKRN
                     HFLYVYYTNREPLENRVARFRVRDNRCRDKRVIVKGLSTKGATNHNGGQLEFMGGKLF
                     VSTGEAADPAEAQDVDSRLGKILRYNPDGSIPDGNPFSRAGDRNPVWSYGHRNPFGLA
                     ARPGTNRLYSTENGPSCDDELNRIRRGANYGWGSGYECGTRGVGPSPRGPIRRWSDIV
                     VPTDVTWYEGRIKSLSGSLYAGDFDGNLHRFFMNAKGTRVREDRVIHSASAIVDVAKG
                     PGGWLYFVTPDAIYRIVS"
     gene            1303..2829
                     /locus_tag="VEV43_06270"
     CDS             1303..2829
                     /locus_tag="VEV43_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYP23158.1"
                     /translation="MARSWAERCASIVVLAGAGVLAPAAAHPPPAPGLFFSHHPHMRA
                     APVVFVVLDELPLVSLLDRRGTIDEKLCPGVARLARMSAWYRNATTPQTFTKEALPAL
                     LTGSYPIRRVGETFEYPRNLFSLLGATYEIRAANVPPNLCPPELCDAPIRPPPVQRFR
                     GFGGGEKAALFFSFLSGLERPDRPRLHFLHLVFPHGPWRYLPSGQRYYEVDPMPGEVD
                     RRGRGRSWRRDGWLVAQGYQRHLMQVRLADRLVDALLVRLKRTGLLHDAVVVVTADHG
                     IGWEPGLPKRLPHERTAATLAGVPLFVKAPGQRVGWVSRTPVESVDVLPTLADILGVE
                     TWPDVDGLSLWAHAPRRARPRAVVDVPIEGFGRQLKRAAREKHELVGRNLWRVAPGES
                     EELVGLTEAELSIAPPGKVTAHASAVETLVRADPQGDEFPAFFEGLLEGVPFRRRPRV
                     AVMVDGRIAAVTRSYESSGLKWFGAMLRPGLFGPHEERVELFLVDDVGGRALTELPLE
                     "
     gene            complement(2830..3315)
                     /locus_tag="VEV43_06275"
     CDS             complement(2830..3315)
                     /locus_tag="VEV43_06275"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HYP23159.1"
                     /translation="MGSRRDGGLRDKTESFFGHVEDVLYVAIATALALVGVGLFGYVV
                     YDFVSKMGDGDFTKKILSLLDGLLLVFIITELIHTVRAVIDERVLVAEPFLLVGIVAA
                     IRRLVVVGAEAKDLLGKPEFGDAMTEIGILTGTVVLLAFAIFLLRNTEKSEPTSEHEP
                     G"
     gene            3343..4779
                     /locus_tag="VEV43_06280"
     CDS             3343..4779
                     /locus_tag="VEV43_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HYP23160.1"
                     /translation="MKAEPLYRLDRALGEALERSLRAHHRRRLRKIGWDHVFDPPEEW
                     ATGGSPPRAGTSLEVLVDGEEAFGAMADAIAGARDHVHVAGWHIEPDFDLAEHGTLEE
                     TLAEAGRNADVRVLSWAGAPLPPPFRPRRAEAAAVAERLGRLNGVRVALDSKERLLHC
                     HHEKLVIVDGTTAFVGGFDFSTLGASRLDGSHHPPKEPMGWHDAAFRIEGPLVRDVAA
                     HFALRWAENAGEYPPVGSPPPSGDVEAQLVRTVPENVYDGLPRGDFTLLASYVAALRE
                     ARELVYLENQFLWSSEVVRILAEKLRNPPSDDFRLVLVLPSKPTTGKDDTLGQLAVLA
                     EADDGAGRFVASTLHSRDGDRTTPVYVHAKGGIVDDRWLTSGSGNLNNHSLFNDSEVN
                     VVTRDEGLARETRLRLWAEHLEVEVESLEGRAPAEIVDDVWIPTALEQLERREAGEPP
                     THRLARLPHVSKRSKRLLGPLQTFLVDG"
     gene            4815..5036
                     /locus_tag="VEV43_06285"
     CDS             4815..5036
                     /locus_tag="VEV43_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HYP23161.1"
                     /translation="MDRARITPYKDGPLLVRGPFELVDQDGNPIEVGRKTIALCRCGR
                     SRIKPFCDGTHKATKFTAESGRCAPPDGA"
     gene            complement(4993..5973)
                     /locus_tag="VEV43_06290"
     CDS             complement(4993..5973)
                     /locus_tag="VEV43_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYP23162.1"
                     /translation="MREKLTYVLPFKASGDEDLDDLTGYLQGLSLVADVIVVDGSEPE
                     TFARHDALWKDHVTHVPPRPGLAFANGKVDGVITGVELAHTEKVVVADEDVRYDVAAL
                     QRMSELLESYDLVRPQNYFDPMPWHALWDTGRILLNRALAADYPGTLGVRRSLFLDAG
                     GYDGDTMFENLEMIRTMEEAGATSVAPLDLYVRRIPPESRHFLAQRPRQAYDDLAQPW
                     RLVTFASIVPGAVVAARRRPSLIPLGALVIAGLAEVGRRRAGGTRVFPAAASMAAPVW
                     VTERAVCTWIALWHRALGGVPYRGSRLKVSANPRRVIRRRLAGRTGHSLR"
BASE COUNT          928 a         2159 c         2181 g          880 t
ORIGIN      
        1 aggtagtacg cccggaggga ttcgaacccc cgaccttccg gtccgtagcc ggacgctcta
       61 atccactgag ctacgggcgc atcagcctgc cacactagcg cgcggaggcg cggggatttg
      121 aaccccggaa ggggtgtaac ccccttaacg cattagcagt gcgctccctt cggccgctcg
      181 ggcacgcctc cgccaacgat catagcgggc ggcaccggcg agaatggctg gccatgagac
      241 gactattgcc gctcctgacc atcgtcgccc tcgccgccac cgcgctgccc ggaccgaccg
      301 ccggcgcggt cccgaagggg acgcgcgtcc agctctaccg cggtgacctc gacttcccca
      361 tcgacatggc gtgggtgccg ggcaccaaga aggtgttctt caccgagaag aacacggggg
      421 ggatccgcgt catggccgga cgccggctgc tcgcgcggcc ctgcgccgac ctcgacgtcg
      481 agccgtcggg tgagcgcggc gcgctcggga tcgtcctgca cccgaagttc aagaggaacc
      541 acttcctcta cgtctattac acgaaccgcg agccgctcga gaaccgcgtc gcgcgcttcc
      601 gcgtgcgcga caaccgctgc cgggacaagc gcgtgatcgt caaggggctg tcgacgaagg
      661 gcgcgaccaa ccacaacggc ggacagctcg agttcatggg cggcaagctg ttcgtctcga
      721 ccggcgaggc cgccgaccct gcggaggccc aggacgtcga cagccggctc ggcaagatcc
      781 tgcgctacaa ccccgacggc agcatccccg acggcaaccc gttctcgcgc gccggagatc
      841 gcaaccccgt gtggagctac gggcaccgca acccgttcgg gctcgcggcg cggcccggca
      901 cgaaccgtct gtactcgacc gagaacgggc cgagctgcga cgacgagctc aaccgcatac
      961 gccgtggcgc gaactacggc tggggtagcg gatacgaatg cgggacgcgt ggagtcgggc
     1021 cgagcccgcg ggggccgatc cggcgctggt ccgacatcgt cgtgccgacc gacgtcacct
     1081 ggtacgaggg ccggatcaag tcgctctccg gctcgctgta cgcgggtgac ttcgacggga
     1141 acctccatcg cttcttcatg aacgcgaagg ggacccgcgt gcgggaggac cgcgtgatcc
     1201 attccgcgtc ggcgatagtc gacgtcgcga aggggccggg cgggtggctg tacttcgtga
     1261 cgccggacgc gatctaccgg atcgtctcgt gaagaggtag ccgtggcgcg ctcgtgggcg
     1321 gagcgatgcg cgtcgatcgt ggtcctcgct ggggcgggcg tcctcgcccc cgcagccgcg
     1381 caccctccgc ccgcgccagg gctgttcttc tcgcaccacc cgcacatgcg cgccgccccg
     1441 gtggtgttcg tcgtgctcga cgagctcccg ctcgtgtcgc tcctcgaccg ccgcgggacg
     1501 atcgacgaga agctctgtcc cggggtcgcg cggctcgcgc gcatgtcggc ctggtatcgc
     1561 aacgcgacga cgccccagac gttcacgaag gaggccttgc ccgcgctgct caccggcagc
     1621 tatccgatcc ggcgcgtggg ggagacgttc gagtatccgc gcaacctctt ctcgctgctc
     1681 ggcgccacgt atgagatccg cgctgcgaac gtcccgccga acctgtgccc gccggagctc
     1741 tgcgacgcgc cgatcaggcc accgcccgtg cagaggttcc gcggcttcgg cggtggcgag
     1801 aaggcagcgc tgttcttctc gttcctgtcc gggctcgagc gtcccgaccg cccgcggctg
     1861 cacttcctcc acctcgtgtt cccgcacggg ccgtggcgat acctgccctc ggggcagcgt
     1921 tactacgagg tcgacccgat gcccggggag gtcgaccggc gggggcgcgg caggtcgtgg
     1981 cggcgtgacg ggtggctcgt ggcgcagggg taccagcggc atctcatgca ggtgcggctc
     2041 gcagaccggc tcgtcgacgc gttgctggtg aggctgaagc ggacgggcct gctgcacgac
     2101 gcggtcgtgg tcgtgacggc ggaccacggg atcggatggg agccggggct gccgaaaagg
     2161 ctgccgcacg agcggaccgc cgcgaccctc gcgggggtgc cgctgttcgt gaaggcaccg
     2221 gggcagcgcg tcgggtgggt ctcgcgtacc ccggtcgaga gcgtcgacgt cctcccgacg
     2281 ctcgccgaca tcctcggcgt agagacgtgg ccggacgtag acgggctctc gctgtgggcg
     2341 cacgcgccgc ggcgggcgag gccgcgcgcg gtggtcgacg tgccgatcga ggggttcggg
     2401 cggcagctga aacgcgcggc ccgcgagaag cacgagctcg tcggccggaa cctgtggcgc
     2461 gtggcgccgg gagagtcgga ggagctcgtc gggctgaccg aggcggagct gtcgatcgcg
     2521 ccgccgggca aggtgacggc gcatgcgagc gcggtggaga cgctggtccg agccgatccc
     2581 cagggagacg agttcccggc cttcttcgag ggactgctgg agggagtccc gttccggcgg
     2641 aggccgcgcg tggcggtcat ggtcgacggc cggatagcgg cggtgacgcg gtcgtacgag
     2701 tcgagcgggc tcaagtggtt cggggcgatg ctgcggccgg gcctcttcgg cccccacgag
     2761 gaacgcgtgg aactgttcct cgtagacgac gtcgggggcc gggcccttac ggagctgccg
     2821 ctcgaatgac taccccggct cgtgctcgga ggtcggctcg ctcttttccg tgttgcgcag
     2881 gaggaagatc gcgaaggcga gcaggacgac ggttcccgtg aggatcccga tctcggtcat
     2941 cgcgtcgccg aactcgggct tgccgaggag gtccttcgcc tccgcgccga cgactacgag
     3001 ccggcggatc gccgcgacga tgccgacgag gaggaacggc tccgcgacta ggacccgctc
     3061 gtcgatcacg gcccgcacgg tgtggatgag ctcggtgatg atgaacacga gcagcaggcc
     3121 gtcgagcagc gacaggatct tcttcgtgaa gtcgccgtcg cccatcttcg agacgaagtc
     3181 gtagacgacg tagccgaaca aaccgacccc gaccagcgca agcgcggtgg cgatcgccac
     3241 gtagaggacg tcctcgacgt ggccgaagaa cgactcggtc ttgtcccgca gaccaccgtc
     3301 ccggcgtgac cccatgaggg caacctaata ggttccgacc ggatgaaggc cgaaccgctg
     3361 tatcggctgg accgcgcgct cggcgaggcc ctcgagcgga gcctccgcgc ccaccaccgc
     3421 cggcgtctgc gcaagatcgg ctgggaccac gtcttcgatc cgccggagga gtgggcgacg
     3481 ggcggctcgc cgccgcgggc cgggacctca ctcgaagtgc tcgtcgacgg cgaggaggcg
     3541 ttcggcgcga tggcggacgc gatcgccggc gcgcgcgacc acgtccacgt cgccggctgg
     3601 cacatcgagc cggacttcga cctcgcagag cacgggacgc tcgaggagac gctggccgag
     3661 gccggccgga acgcggacgt gcgcgtgctg tcgtgggccg gggccccgct gcccccgccg
     3721 ttccgtccgc gccgcgcgga agccgctgcc gtcgcggagc ggctcggccg gctcaacggg
     3781 gtgcgcgtcg cgctcgactc caaggagcgc ctcctccact gccatcacga gaagctcgtg
     3841 atcgtcgacg gcacgacggc gttcgtcgga ggcttcgact tctcgacgct cggcgccagc
     3901 cgtctcgacg ggtcgcacca tcctccgaag gagcccatgg gctggcacga cgccgcgttc
     3961 cgcatcgaag gtccgctcgt gcgcgacgtc gccgcgcact tcgcgctccg ctgggccgag
     4021 aacgccggcg agtatccgcc cgtcggatct ccgccgccgt cgggcgacgt agaggcccag
     4081 ctggtgcgca ccgtgcccga gaacgtctac gacggtctgc cgagaggcga cttcaccttg
     4141 ctggcgagct acgtcgccgc gctccgcgag gcgcgcgagc tcgtctatct cgagaaccag
     4201 ttcctgtggt cgtcggaggt cgtcaggatc ctcgcggaga agctgcgcaa ccctccgagc
     4261 gacgacttcc gcctggtcct cgtgctcccg tcgaagccga cgaccggcaa ggacgacacg
     4321 ttgggacagc tcgccgtcct cgcggaggcc gacgacggcg cgggacggtt cgtcgcgtcg
     4381 actctgcact cgcgcgacgg cgatcgcacg acgcccgtct acgtgcacgc caagggaggc
     4441 atcgtcgacg accggtggct cacgagcggc tcgggcaacc tcaacaacca ctcgctgttc
     4501 aacgactcgg aggtcaacgt cgtgacgcgc gacgaaggac tggcgcgcga gacgcgcctg
     4561 cggctgtggg cggagcacct ggaggtcgaa gtcgaatcgc tcgagggccg cgcccccgcc
     4621 gagatcgtcg acgacgtgtg gatcccgacg gccctcgagc agctcgaacg ccgcgaggcc
     4681 ggggagccgc cgacccaccg cctggcgcgc ctcccgcacg tgtcgaagcg ctcgaagcgc
     4741 ctgctgggcc ccctgcagac gttcctggtc gacggctgag gttgccgccc tcgcctcggg
     4801 ggcataacgc ggtcgtggac cgcgcccgga tcactcccta caaggacggg cccctgctcg
     4861 tgcgggggcc gttcgagctc gtcgaccagg acggcaaccc gatcgaggtc ggccgcaaga
     4921 cgatcgcgct ctgccgctgc ggccgctcgc gcatcaagcc cttttgcgac ggcacgcaca
     4981 aggcgacgaa gttcaccgca gagagtggcc ggtgcgcccc gccagacggc gcctgatcac
     5041 gcgccgcggg ttcgccgaga ccttcagcct gctgccgcgg taggggacgc cgccgagggc
     5101 ccggtgccat aacgctatcc aagtgcagac ggcgcgctcg gtgacccaca ccggcgccgc
     5161 catcgacgcc gccgcgggga acacccgcgt cccgcccgcc cgccggcgcc cgacctcggc
     5221 gagcccggcg atcacgagcg ccccgagcgg gatcagcgac ggacgtctgc gggccgcgac
     5281 cacggctccc gggacgatcg acgcgaacgt cacgagcctc cagggctggg cgaggtcgtc
     5341 gtaggcctgg cgcggccgct gcgcgaggaa gtgacgtgac tccggtggga tccgccgcac
     5401 gtacaggtcg agcggggcga ccgaggttgc gccggcctcc tccatcgtgc gaatcatctc
     5461 gaggttctcg aacatcgtgt ccccgtcgta tccgcccgcg tcgaggaaga gcgaacgcct
     5521 cactccgagc gtgccgggat agtcggcggc cagcgcacgg ttgagcagga tccgtcccgt
     5581 gtcccacagc gcgtgccacg gcatcgggtc gaagtagttc tgcggccgca cgaggtcgta
     5641 cgactcgagc agctcgctca tccgctggag cgcggctacg tcgtagcgga cgtcctcgtc
     5701 ggcgacgacg accttctccg tgtgcgctag ctcgacgccg gtgatcacgc cgtcgacctt
     5761 gccgttcgcg aaggccagac ccggccgcgg cggaacgtgc gtcacgtggt ccttccacag
     5821 cgcgtcgtga cgcgcgaacg tctccggctc cgatccgtcg acgacgatga cgtcggcgac
     5881 gagtgaaagg ccctgcaggt accccgtcag gtcgtcgagg tcctcgtcgc cggacgcctt
     5941 gaagggcagg acgtacgtca gcttctcgcg cacgagctac agcagcgagg atcggccggc
     6001 gtcccacgcc gacatcaggt gctcgcccca gaggccctcg agatgcatca ggcagcgcgc
     6061 gccgagctcg acgtccgggg cgaggttggg ctcgtcgcgc tcgatcgacc cggcgaggtc
     6121 gtacgccgcc acgttctcgt ggaccgcg
//
LOCUS       DASQDO010000218         7877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_327795, whole genome shotgun sequence.
ACCESSION   DASQDO010000218 DASQDO010000000
VERSION     DASQDO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327795"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..758)
                     /locus_tag="VEV43_06295"
     CDS             complement(78..758)
                     /locus_tag="VEV43_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23163.1"
                     /translation="MNDVHAPEKVRCQSCGAKNPPDAEWCGQCLKRFQAPPPPPPVAA
                     PASVTSGAPASAGSVKPGVERGNFRSTDEGILWRCTSCDHENPLEDPACAVCGTPFAQ
                     AVRPPEPERAQRDPGTVALISLFFPGAGHAYLGLWPQAIARAVVSAWVMLMAGVFAIG
                     VGVVSIAAVYGLAAFGLWGIAAHDAFREANGEPKRVLLQGRRFTYLVLGLLSVLLASI
                     FLMSFAGR"
     gene            complement(822..1619)
                     /gene="panB"
                     /locus_tag="VEV43_06300"
     CDS             complement(822..1619)
                     /gene="panB"
                     /locus_tag="VEV43_06300"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYP23164.1"
                     /translation="MPVTVNDVRAYKERGEKFVMLTAYDTPSARLLDEAGIPLILVGD
                     TMGMVLLGYGSTVPVTMDEVVHHTAAVARGTTNALVVGDMPFMSYQISVEDGMRNAGR
                     LLQEAGANAVKLEGAGRSLDLVERLVEAGIPVMGHLGLTPQSVNQLGGYKVQGRTEEA
                     HHKLIEDAKSLEAAGIFSLVLEAVPEPLAAEVTTSVAVPTIGIGAGRYCDAQVLVWHD
                     FLGITDGHLPRFVKRYANLGEEIKRAAQEFAAEVAEGAYPGPEHVYR"
     gene            complement(1719..2369)
                     /locus_tag="VEV43_06305"
     CDS             complement(1719..2369)
                     /locus_tag="VEV43_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23165.1"
                     /translation="MNKRTCSIVLALGLVVSGVIAGPATAGKKKKPKPKVCTAFQPGE
                     LGAEAETVVVTDAHTAEAPLLHAFSLDMAFDEGVNDVLNEEAGTPLPGTPHHYVNIQV
                     DSKAATAGLYVTWEFDSNRDYDLWAYNADGTEAASAHGFQPLIATEGQPEPADQSNTG
                     TNHAGHSTATTENIVGLITNDCGGYTIDTATYFGEGGDFELKIWLGEGTTAPGVPE"
     gene            complement(2495..2941)
                     /locus_tag="VEV43_06310"
     CDS             complement(2495..2941)
                     /locus_tag="VEV43_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23166.1"
                     /translation="MRRHLLVLAIGAIAIGATGVTPASADAPPWPINCVTDYGTVPPG
                     AQSVYVDSSGRLVVNPEAAPGDVDAFAAWAPDKLLAMYPCLVGPVFIDGVVCTYQKAF
                     EAVDSLDPTNSYLRYVYPNPNGPGYVVDHPRLLADVSHVVGCLATV"
     gene            complement(3032..3508)
                     /locus_tag="VEV43_06315"
     CDS             complement(3032..3508)
                     /locus_tag="VEV43_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23167.1"
                     /translation="MRVSRLLIACLVAACSAVPLASPALAQDPECPSDWAGQYLLSLI
                     PPIPAFEPVEFDEETITIRGDLVAGEAAALIDHYVSNTETFLTCLQEYVDGLVSPYAD
                     CVSERSAPVLTSPDPVGRYVEVGADLVVKVHYAQAMADVVAIFNCNDIVTYGDTQR"
     gene            complement(3621..4328)
                     /locus_tag="VEV43_06320"
     CDS             complement(3621..4328)
                     /locus_tag="VEV43_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23168.1"
                     /translation="MKRIVTVVAVAGLVASALHAAPAAAQTEVTVPEVVNIEDPFGDA
                     NSLGGDQQLPAGDASTVSDLGKVWFSHDATNFNVHILTEGPPAANTVGLQFVVTGGPE
                     GCAVFSGYFNGLTYLSDAFSRFVDNCNSLDPIDGTFTFVEGPEGRGLATLTIPRAGTP
                     FLADGAAVSAPVVQSWIFAGGEQLTPSGYRGVRQMVDDTKPGTDYAIAAEEPVDEPEP
                     TKPKKPKKPKKPKKPKP"
     gene            complement(4509..6380)
                     /locus_tag="VEV43_06325"
     CDS             complement(4509..6380)
                     /locus_tag="VEV43_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYP23169.1"
                     /translation="MPAETIIEKFQERIAEHPDAVALKYKSGGAWKDITWRDYGDRVR
                     RAAKGLIAQGLGHGDKMSLLSLNRPEWHVADVACLSIGAATAPIYVTNSPEQVAYIVA
                     HSESKVAVVENPDQLQKILKMRSELPALKKVVVIEGYGGDADPDFVMSWDDFLKAGDT
                     IADADFDAARAKVEPKDLATFVYTSGTTGPPKAVMLTHANVMWTARSSEAIIPLGEAT
                     KGKAVSYLPLSHIAERMLTHFLQIYYGTTTCFAESLDTLLVDLQAIKPTYFFGVPRVW
                     EKFYAGVQAKMAQADPNDRKTKLARKAIGLGKEITKLEQEAVERGGRTTDAKIPLGMK
                     LQHAALDKLVLHKIRAAFGLEECELALSSAAPLSPELIWFFHSIGLKIAEGYGQSEDN
                     GPTSWNPPDAILIGSVGKPMPGLEVKLAEDGEILARGGNVMAGYFKDDKATRETIDED
                     GWLHSGDVGEFNEHGFLKITDRKKDLIITAGGKNIAPQEIENKIKVHSLISQVVAIGD
                     RRPFMTCLITLDPEKAPVWAKEQGIDGDMAAIANHERTIKEIEGAINETNNGLAKVEQ
                     IKKFRILERDFLQEEDEITPTMKVKRKTINEIYGDAIEEMYSKESPPGAAIKAPLHK"
     gene            complement(6447..7712)
                     /locus_tag="VEV43_06330"
     CDS             complement(6447..7712)
                     /locus_tag="VEV43_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYP23170.1"
                     /translation="MTWARALLTIAAVSIAAAALVGLVSRVPAEVRGRQADPSATDPE
                     LGSSFTDEQVSRHAAYQRPGYVSFAAGLVVQVVTLVVLARGPWAKLIEGLEDRIGAWP
                     LRALVAGALAGLVLTAIALPLAFVRGYTIEHAWGLSTQDVGGWLADRAKGAVIGAVTS
                     AVAALVFFAVVRWQPRTWWVFGWAAFTGLTAVMFFLWPVVIAPLFNKFTPLEPGPLDD
                     RVRALAEEAGVPVDEVLVADASRRTTAENAYVAGLGKTKRLVVYDTLLKANDDDRTAF
                     VVAHELGHQAKSHVPKNLAIASAGLLAAFAFLGWLGGRGSFWGWAGADGPGDPRALPL
                     LVLLSIGLTLVSTPLESALSRRDETEADRFAIELTGDPDAAVAAFRRLALANLAYLDP
                     PPLAVAAFYSHPPIPDRIDAVLATAGKHH"
BASE COUNT         1139 a         2634 c         2783 g         1321 t
ORIGIN      
        1 cgtcacggtg cagcgttcag cggaggatcg tgggacgtcg aggggccgga cgggccggtg
       61 gaggctcggc gcctcggcta gcgacccgcg aaggacatca ggaagatcga cgctaggagc
      121 acgctcaaga ggccgaggac gaggtacgtg aagcggcggc cctggaggag gacccgcttg
      181 ggctcgccat tcgcctcgcg gaacgcgtcg tgcgcagcga tcccccacag cccgaacgcc
      241 gccagcccgt agacggccgc gatcgacacc accccgacgc ctatggcgaa gacgccggcc
      301 atcagcatca cccacgcgct gacgaccgcg cgcgcgatcg cctgcggcca cagccccagg
      361 tacgcgtgcc cggcccccgg gaagaacagc gagatcagcg cgaccgttcc ggggtcgcgc
      421 tgcgcgcgct cgggctccgg cggccgtacc gcctgcgcga acggagtccc gcacacggcg
      481 cacgcggggt cctcgagggg gttctcgtgg tcacaggacg tgcagcgcca caggatcccc
      541 tcgtcggtcg aacggaagtt cccgcgctcg accccgggct tcacggaacc ggcggacgcg
      601 ggcgcgcccg acgtaacgga cgcaggagcg gcgaccggcg ggggcggcgg aggggcctgg
      661 aaccgcttca ggcactggcc gcaccactcg gcgtccggcg ggttcttcgc tccgcaggac
      721 tggcaccgca ccttctcggg cgcgtggacg tcgttcatgc tcatcgggta cttcatcggc
      781 gtccgccggg ggtgactcaa gccccgtaac ggacaggccg gtcagcggta tacgtgctcg
      841 ggtcccgggt aggcgccctc ggcgacctcg gcggcgaact cctgcgcggc ccgcttgatc
      901 tcctcgccca ggttcgcgta gcgcttgacg aagcgcggga ggtggccgtc ggtgatcccg
      961 aggaagtcgt gccacacgag gacctgggcg tcgcagtagc ggcccgctcc gatgccgatc
     1021 gtcgggaccg ccaccgaggt cgtgacctcg gccgctagtg gctcgggcac ggcttcgagg
     1081 acgagcgaga agatgcccgc ggcttcgagg ctcttggcgt cctcgatcag cttgtggtga
     1141 gcttcctcgg tgcggccctg caccttgtag ccaccgagct ggttgacgga ctgcggggtg
     1201 aggccgaggt gccccatcac ggggataccc gcctcgacga gccgttcgac gaggtcgagc
     1261 gaccggccgg ccccctcgag cttgacggcg ttcgcgccgg cctcctggag aagtcgtccg
     1321 gcgttgcgca tgccgtcttc gaccgagatc tggtagctca tgaacggcat gtcgccgacg
     1381 accagggcgt tggtcgtccc gcgcgcgacg gcggcggtgt ggtgcacgac ctcgtccatc
     1441 gtcacgggga ccgtggagcc gtagcccagc aggaccatcc ccatcgtgtc gccgacgagg
     1501 atcaagggga tcccggcctc gtcgaggagc cgggcggacg gcgtgtcgta ggccgtcagc
     1561 atcacgaact tctcgccgcg ctctttgtac gcgcggacgt cgttcaccgt tacgggcact
     1621 gtcgcctcct tgcacaggta gccgcagctc cgtccagtgt acgggcaaaa caaaagaggg
     1681 gcccctcgag ggcccctctt tggagtcttg cttggtggtt actcggggac gccgggagcg
     1741 gtcgtgccct cgccgagcca gatcttcagc tcgaagtcgc cgccctcacc gaagtaggtg
     1801 gccgtgtcga tcgtgtagcc accgcagtcg ttggtgatga gcccaacgat gttctcggtc
     1861 gtcgccgtgg agtgacccgc gtggttcgtc ccggtgttcg actggtccgc cggctcgggc
     1921 tggccctcgg tcgcgatcag cggctggaag ccgtgcgccg aggcagcctc ggtgccgtcg
     1981 gcgttgtagg cccacaggtc gtagtcacgg ttggagtcga actcccacgt gacgtacagg
     2041 ccggcggtgg ccgccttcga gtcgacctgg atgttcacgt agtggtgcgg ggttcccggc
     2101 agcggcgttc cggcctcctc gttgaggacg tcgttgacgc cctcgtcgaa ggccatgtcg
     2161 agcgagaacg cgtggagcag gggggcctcg gcggtgtggg cgtcggtgac gacgaccgtc
     2221 tcggcctccg cgcccagctc gcccggctgg aatgccgtgc ataccttggg cttgggcttc
     2281 ttcttcttgc cggcggtggc ggggccggcg atgacgccgg agacgaccag gccgagggcc
     2341 aggacgatcg agcaggtgcg cttgttcatg acgagattcc ccttttcgca gttcggctac
     2401 gaggtcgttt cagggctttc tgggttattc gccgccgcgg gatggcctcc tgcggccggt
     2461 tttcgggggg cgccctgtcc tcgccgacgg ccactcacac cgtggccagg caccccacga
     2521 catgcgagac gtcggccaag aggcgcgggt ggtcgacgac gtatccgggg ccattcgggt
     2581 tggggtacac gtagcggaga tacgagttgg tcgggtcgag actgtccact gcctcgaatg
     2641 ctttctggta ggtgcaaacg acgccgtcga tgaataccgg cccgacgagg cacggataca
     2701 tggcgagcaa cttgtccggc gcccaggccg cgaaggcatc gacgtcgccg ggcgcggctt
     2761 cgggattcac gaccagtcgt cccgaggagt ccacgtacac ggactgagcg ccgggcggca
     2821 ccgttccgta gtcggtcacg cagttgatcg gccacggagg agcgtctgcg ctcgccggag
     2881 taactccggt cgcaccgatc gcgattgcgc ctatcgccag cacgagaagg tgtcgtctca
     2941 ccgcaaggct cctttgcctc gtccacacgg ctttcgccgt cccccctgat gagtcttctc
     3001 ggaactccgt cgtatccctt tcggcccccg gctacctctg ggtgtcgccg taggtgacga
     3061 tgtcgttgca gttgaagatc gccacgacgt ccgccatcgc ctgcgcgtag tgcaccttca
     3121 cgacgaggtc cgcccccacc tcgacgtagc ggccgacggg gtccgggctc gtgaggacgg
     3181 gggccgacct ctcggaaacg cagtcggcgt aggggctgac gaggccgtcc acgtactcct
     3241 gcagacacgt caggaacgtc tcggtgttcg agacgtagtg gtcgatcagg gccgctgcct
     3301 cgccggcgac gaggtcgccc cggatcgtga tggtctcctc gtcgaactcg accggctcga
     3361 aggccgggat cggcgggatc aaagagagca ggtactggcc cgcccagtcc gaggggcact
     3421 cggggtcctg tgcgagggcc ggtgacgcga gaggcacggc cgagcaggcc gcgactaagc
     3481 acgcgatgag aaggcgcgag acgcgcatga ggggaacctc cgattgttcg tccgccgtga
     3541 ctccccgaag gttcgacggc tcgcttcgct tgtcctgccg ggaacgcacg aggggccccc
     3601 gttcgggggc cctctcgagg ttacggcttc ggcttcttcg gcttcttggg cttcttcggc
     3661 ttcttgggct tggtcggctc gggctcgtcg acgggctcct ccgcggcgat cgcgtagtcc
     3721 gtgcccggct tcgtgtcgtc gaccatctgc cggacgcccc ggtagccgga cggcgtcagc
     3781 tgctccccgc cggcgaagat ccacgactgc accaccgggg ccgacaccgc ggcgccgtcg
     3841 gcgaggaacg gcgtgcccgc gcgcgggatc gtcagagtcg ccagcccgcg gccctcaggg
     3901 ccttcgacga acgtgaacgt tccgtcgatc gggtcgaggc tgttgcagtt gtcgacgaac
     3961 cggctgaacg cgtcggatag gtacgtcagg ccgttgaagt agccgctgaa aacggcgcaa
     4021 ccctcgggcc ccccggtgac gacgaactgg aggccgaccg tgttcgcagc cggcggcccc
     4081 tcagtgagga tgtggacgtt gaagttcgtg gcgtcgtggc tgaaccacac cttgcccagg
     4141 tcggacacgg tgctcgcgtc accggccggc agctgctggt cgccgcccag cgagttcgcg
     4201 tcaccgaacg ggtcctcgat gttgacgacc tcgggcacgg tcacctcggt ctgggccgct
     4261 gcgggtgcag cgtgcaaggc cgacgcgacc aaccccgcga cggctaccac tgtgacgatc
     4321 ctcttcatgc tcgactcccc agtcgtcggg ccgaaagcgc ccgctccacc atccgaccgc
     4381 caagacttcg tcggaccgac gcacgactcc ttctccggcc cgggctcggc ttgggctatc
     4441 acctgcgagc atcggaaggc caacgaccaa gggccccctt tcgggggccc ttgtcttcgg
     4501 tccggtcgtt acttgtgcag cggcgccttg atcgcggccc ccgggggtga ctccttcgag
     4561 tacatctcct cgatcgcgtc gccgtagatc tcgttgatgg tcttgcgctt caccttcatc
     4621 gtcggcgtga tctcgtcctc ctcctggagg aagtcacgct cgaggatgcg gaacttcttg
     4681 atctgctcga ccttggcgag gccgttgttg gtctcgttga tcgcgccctc gatctccttg
     4741 atcgtccgct cgtggttcgc gatcgcggcc atgtcgccgt cgatcccctg ctccttcgcc
     4801 cacaccggcg ccttctcggg gtcgagcgtg atcaggcacg tcatgaacgg acggcggtcg
     4861 ccgatcgcga cgacctggct gatgagcgag tggaccttga tcttgttctc gatctcctgc
     4921 ggggcgatgt tcttgccgcc ggcggtgatg atcaggtcct tcttgcggtc ggtgatcttg
     4981 aggaagccgt gctcgttgaa ctcgccgacg tcgccggagt ggagccatcc gtcctcgtcg
     5041 atggtctcgc gcgtcgcctt gtcgtccttg aagtagccgg ccatcacgtt gccgccgcgg
     5101 gcgaggatct cgccgtcctc cgcgagcttg acctcgaggc ccggcatcgg cttcccgacc
     5161 gaaccgatga ggatcgcgtc gggcgggttc cacgaagtcg ggccgttgtc ttccgactgg
     5221 ccgtagccct cggcgatctt gaggccgatc gagtggaaga accagatgag ctcgggcgac
     5281 agcggggccg ccgacgacag cgcgagctcg cactcctcga gcccgaacgc ggcgcggatc
     5341 ttgtgcagca cgagcttgtc gagggccgcg tgctgcagct tcatgccgag cgggatcttc
     5401 gcgtcggtcg tccgtccccc gcgctcgacg gcctcctgct cgagcttggt gatctccttg
     5461 ccgagcccga tggccttgcg cgcgagcttc gtcttgcggt cgttcgggtc ggcctgtgcc
     5521 atcttcgcct gcacgcccgc gtagaacttc tcccacacgc gcgggacgcc gaagaagtac
     5581 gtcggcttga tcgcctggag gtcgacgagc agcgtgtcga gcgactccgc gaagcacgtc
     5641 gtcgtgccgt agtagatctg gaggaagtgc gtgagcatgc gctctgcgat gtgcgacaga
     5701 ggcaggtagc tgacggcctt gcccttcgtc gcctcgccga gcgggatgat cgcctcggag
     5761 ctgcgcgcgg tccacatgac gttcgcgtgc gtgagcatga cggccttggg cgggccggtc
     5821 gtgcccgacg tgtagacgaa cgtcgcgagg tccttcggct cgaccttggc gcgtgcggcg
     5881 tcgaagtccg cgtccgcgat cgtgtcgccc gctttgagga agtcgtccca gctcatgacg
     5941 aagtcggggt ccgcgtcgcc gccgtagccc tcgatcacga cgaccttctt cagcgccggg
     6001 agctccgagc gcatcttgag gatcttctgg agctggtcgg ggttttcgac gaccgcgacc
     6061 ttggactcgg agtgggccac gatgtacgcg acctgctcgg gcgagttcgt cacgtagatc
     6121 ggcgcggtgg cggcaccgat cgacaggcac gcgacgtcgg cgacgtgcca ctcgggacgg
     6181 ttcagggaga gcagcgacat cttgtcgccg tggccgaggc cctgggcgat gagccccttg
     6241 gcggcgcggc ggacccggtc gccgtagtcg cgccacgtga tgtccttcca ggcgccgccc
     6301 gacttgtact tgagggcgac cgcgtcgggg tgctcggcga tgcgctcctg gaatttctcg
     6361 atgattgtct ctgcgggcat gggtcccccc tgatcttggg cgcggtcggg agcagcgttg
     6421 ctccagcaag acgagtgggt cccagtttag tggtgttttc ccgctgtggc caagacggcg
     6481 tcgatccggt ccgggatggg cggatggctg tagaacgccg ccacggctag cggcgggggg
     6541 tcgaggtagg cgaggttcgc cagcgcgagg cggcggaacg ccgccaccgc cgcgtcgggg
     6601 tcgcccgtga gctcgatcgc gaaccggtcg gcctcggtct cgtcgcgcct cgacagcgcg
     6661 gactccagcg gggtcgagac gagcgtgaga ccgatcgaca ggaggacgag cagcgggaga
     6721 gcccgcgggt cgcccggccc gtccgctccg gcccagcccc agaacgagcc ccggcccccg
     6781 agccagccga ggaaggcgaa cgcggcgagg agcccggcgc tcgcgatcgc gaggttcttc
     6841 ggcacgtggc tcttcgcctg gtggccgagc tcgtgcgcga cgacgaacgc ggtccggtcg
     6901 tcgtcgttcg ccttcaggag cgtgtcgtag acgacgaggc gcttcgtctt gccgagcccg
     6961 gcgacgtagg cgttctccgc ggtcgtacgc cggctcgcgt cggcgacgag gacctcgtcg
     7021 acggggaccc cggcctcctc ggcgagagcc ctcacgcggt cgtcgagggg gccgggctcg
     7081 agcggcgtga acttgttgaa cagcggcgcg atgacgacgg gccagaggaa gaacatgacc
     7141 gcggtgagcc ccgtgaacgc cgcccacccg aagacccacc acgtgcgcgg ctgccacctg
     7201 acgacggcga agaagacgag ggccgcgacg gccgacgtca ccgccccgat cacggccccc
     7261 ttggcgcggt cggcgagcca cccaccgacg tcctgcgtcg agaggcccca cgcgtgctcg
     7321 atcgtgtagc cgcgcacgaa ggcgagcggg agtgcgatcg cggtcaggac cagcccggcg
     7381 agggccccgg cgacgagggc ccggagcggc catgcgccga tgcgatcttc caggccctcg
     7441 atcagcttcg cccacggccc ccgggcgagg acgacgaggg tcacgacctg cacgacgagc
     7501 ccggccgcga acgagacgta gcccggccgc tggtaggccg catgtcgaga gacctgctcg
     7561 tcggtgaagc tggagccgag ctcgggatcg gtagcgctgg gatcggcctg gcgcccccgg
     7621 acctcggcgg gaacccgaga gacgagcccc acgagggccg cggcggcgat ggagacggcg
     7681 gcgatggtca gcagagcccg ggcccaggtc atgggccgag cgtagctaga tcagggccgg
     7741 taccggtcgt cgtcgccgtc ggagccgaag tcgtggaggg cgatcgtgtc gatcacctgc
     7801 gagagcgcct cgtcggggtt gccggcgacg ccgacgtcga aggcaccgtc gggcccgtcg
     7861 accaaggccg tccacgt
//
LOCUS       DASQDO010000219         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_634354, whole genome shotgun sequence.
ACCESSION   DASQDO010000219 DASQDO010000000
VERSION     DASQDO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634354"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1957)
                     /locus_tag="VEV43_06335"
     CDS             complement(<1..>1957)
                     /locus_tag="VEV43_06335"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYP23171.1"
                     /translation="GAHGVTGWLARSLRGAPGVPAEFQLSLQGTALRIAGNHRALLEE
                     TALALSLLDRAGVEAVVLKGPALVERYHRDPTLRSYGDVDLLVRPDRFGAALAAFQEA
                     GYLLADRNWDFLVDDLRGQVHLTTPRDEVIELHWHVVNGSRQRRTLRMSPEEMWDAVE
                     ETTLGGAPARVLAREDELAHLTLHAAMHGCNRLVWLLDVAALMSAGDLDWDRVAARLR
                     RWRFGTGGGLVLSLARHWASAPVPGEVLRALVGAPTRALARRAVDGWDLGAAGDGSQA
                     REAFFATAGDDVGTRAGLALDAVVPARGQQVEAPEGVLYRASVGTFHRIRGKVFHRDH
                     RDAMAEYVSVGDQDEGRRRFVDAVAATAAPPGARRVLLVSPSQSIGMSHYTRALADSM
                     SGATAVEVVDAATGDPLSEVASRWRAADEGVRATTRVLVTSPHWATPALLQATGWRGG
                     FVWHDPILDAATPRTKPLHELYYRLLTRKLGVVVLHGSVFEGRVAELGLPAREVVVVP
                     HGFVPNQLVADDPYDPAGPFVFAGRLHPYKGLDVLLRALASIPGDGAPPVVVAGAGVT
                     RELVPASLASVEVRPGEIPDDELRGLIGAASAVLLPYERANQSGVLATAFRSGRPVIA
                     SDVGSFAEYVRDGHNGLLVPPGDPAALA"
BASE COUNT          265 a          740 c          730 g          222 t
ORIGIN      
        1 cggcgagggc cgccgggtca cccggcggga ccaggagccc gttgtggccg tcgcggacgt
       61 actcggcgaa cgacccgacg tccgacgcga tcacggggcg gcccgatcgg aaggccgtcg
      121 ccagcacgcc ggactggttc gcgcgctcgt aggggagcag caccgcgctc gccgcgccga
      181 tcagcccccg cagctcgtcg tccgggatct ctcccggcct gacctcgacc gacgccaggc
      241 tcgcggggac gagctcgcgc gtcacgcccg ctccggcgac cacgaccggc ggtgctccgt
      301 caccggggat cgacgcgagg gcgcgcagga gcacgtccag gcctttgtag ggatggagcc
      361 gcccggcgaa gacgaagggg ccggccgggt cgtaggggtc gtcggcgacg agctggttcg
      421 gcacgaaccc gtgcgggacg acgacgacct cgcgagcggg caggccgagc tcggcgacgc
      481 ggccctcgaa caccgacccg tgcagcacca cgacgccgag cttgcgcgtc agtagccggt
      541 agtagagctc gtgcagcggc ttcgtacggg gcgtggcggc gtcgaggatc gggtcgtgcc
      601 acacgaaccc gcccctccag ccggtcgcct gcaagagcgc cggcgtcgcc cagtgcggag
      661 acgtgacgag caccctagtc gtcgcccgaa cgccctcgtc cgccgcgcgc caccgcgacg
      721 ccacctcgga cagcgggtcg ccggtcgcgg cgtccacgac ctcgacggcg gtcgcgccgc
      781 tcatcgagtc ggcgagcgca cgcgtgtagt gggacatgcc gatggactgc gacggcgaga
      841 cgaggagcac ccgccgcgcg cccggtggcg cggccgtggc ggcgaccgcg tcgacgaacc
      901 ggcgtcgccc ctcgtcctgg tcgccgacgg agacgtactc ggccatggcg tcgcggtggt
      961 cgcggtggaa caccttgccg cggatccggt ggaaggtccc gacgctcgcc cggtagagca
     1021 cgccctcggg cgcttcgacc tgctggccgc gtgcagggac gaccgcgtcg agggcgagac
     1081 cggcgcgcgt cccgacgtcg tcgcccgcgg tcgcgaagaa cgcctcgcgg gcttgcgacc
     1141 cgtcacccgc ggcccccagg tcccacccgt cgaccgcgcg ccgggccagg gcccgcgtgg
     1201 gcgcgccgac gagcgcccgc aggacctccc ccgggacggg ggccgaggcc cagtgccgtg
     1261 cgagcgagag gacgaggccc cctccggtcc cgaagcgcca ccggcgcagc cgcgcggcga
     1321 cgcggtccca gtcgaggtct ccggcggaca tgagggccgc tacgtcgagc aaccagacga
     1381 gccggttgca gccgtgcatc gcggcgtgca gcgtgaggtg cgcgagctcg tcctcgcggg
     1441 cgagcacacg cgccggtgct ccgccgagcg tcgtctcctc gacggcgtcc cacatctcct
     1501 cgggcgacat gcgcagcgtg cgccgctgcc tcgagccgtt gaccacgtgc cagtggagct
     1561 cgatgacctc gtcccgcggg gtcgtgaggt gcacctgccc ccgcaggtcg tcgacgagga
     1621 agtcccagtt gcggtccgcg agcaggtagc ccgcctcctg gaacgcggcc agcgccgccc
     1681 cgaagcggtc cgggcggacg agcaggtcga cgtcgccgta cgacctcagc gtcgggtcgc
     1741 ggtggtaccg ctcgaccagc gctggcccct tgaggacgac tgcctcgacc ccggcacggt
     1801 cgagcaacga cagcgccagt gccgtctcct cgagcagcgc ccggtggttg ccggcgatcc
     1861 ggagcgccgt cccctggagg gagagctgga actcggccgg gaccccgggg gcgccccgca
     1921 ggctccgggc gagccacccc gtcacgccgt gggcgcc
//
LOCUS       DASQDO010000220        10089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1062676, whole genome shotgun sequence.
ACCESSION   DASQDO010000220 DASQDO010000000
VERSION     DASQDO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062676"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VEV43_06340"
     CDS             <1..779
                     /locus_tag="VEV43_06340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HYP23172.1"
                     /translation="SPLGERLPEAVWIEPIPDAQLMPAGSDPAAAAELRESIRLAFVA
                     ALQHLAPKQRAVLILRDVLGWRASEVAELLGTTVAGVNSALQRARATLANAEVHSGSA
                     PDALDDRTETLLALYVDAFERYDMDRLTSLLHDDATWTMPPYELWLNTHEDIRRWCLG
                     RGIGCRGSRLVPVRANASPAFAQYKPAADGGFDAWSLQVLDVAGRKIRGICFFLDAEW
                     LFPMFGLPPRLPAEPLTMRPRLVRTDELSRDGRSNPTTTR"
     gene            796..1527
                     /locus_tag="VEV43_06345"
     CDS             796..1527
                     /locus_tag="VEV43_06345"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYP23173.1"
                     /translation="MRTILGIWAHPDDEVFTSGGLMADAVRRGDRVVCLHLTRGEAGL
                     YHRQVWPARDLGAVRTAELEASLARLGVTEQRFLDLPDGGLCLVAWDEVVARLHDELV
                     DVDPDVIVTFGSDGFTGHPDHRALSSWVSTAVRLWEKPSARVLQAVVPRAWERTAVHR
                     LNEFDFFWPGYPDFAGPADAGIRLDDELVEAKVDAVKAHASQMDCLFEAYGDDFFRSI
                     LSTEVYRTGVRPAFRSRTLQDARRP"
     gene            1537..1869
                     /locus_tag="VEV43_06350"
     CDS             1537..1869
                     /locus_tag="VEV43_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HYP23174.1"
                     /translation="MAYDEDLADRIRELAAGERALTEKKMFGGLAFLVRGHMAVAASG
                     QGGILVRVDPAESEALLAKTDAYPMEMRGREMRGWLRLDAEHVRTKAQLSRWVRRGLD
                     YARSLPPK"
     gene            1988..3571
                     /locus_tag="VEV43_06355"
     CDS             1988..3571
                     /locus_tag="VEV43_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23175.1"
                     /translation="MAPVTAKAGDETCMVFNTIKGAPQGRVVRFVVGEDKQPREMTQA
                     ETEAELGDPFATLLLNKGVFPSTAAELLDAVDARVGANHPLGPSSHMSFVLGEGSQIP
                     LADATPSTNAGLRFLVSRGSGPKGPDLIISTPGPDIAFVEVMAWDKKRKGFNYYRSAG
                     PKRWAFAGNSRHALTPPTKGKGPFESHPSGNLIMKELRLPWVHWHSFEVDIFEDAFPK
                     GDSRRKHPWFTQKLGAEICETAVVMPSITRWTEARLNQVVAANGTVTDPARVVEQIVT
                     TPTVNLASSSRESKGAGPLDLPPGFFVDFDGLSRIGLPGPPGFSVSRAIYAKSLKTFD
                     FRLDDGDGFVQPGDTHFAFVVPERAFEDTETLAQAVDRGLITRRLAAALLMVDFPNPV
                     FSARRASLLKHAPKTATIKSGKSTYSDDMANAIIAAAAATPANSPEKEFVDRWNKAAT
                     LPGPLGDELEAYYDAIAKKLKTQAGFDDFVRLAESQRKRVRDQTRLVESLLLFPTTDV
                     DDDERRAMKRDATVVLVSA"
     gene            3585..4598
                     /locus_tag="VEV43_06360"
     CDS             3585..4598
                     /locus_tag="VEV43_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23176.1"
                     /translation="MPLKRFAVPGNLKELSKEGLKAWSARLSELFDAVAEPPGRHFYN
                     PTKSDTPPDASVAAVTWPAAPGRLLSQRLAPERRWEIADGDRNQQDEYCEWSVLRDGN
                     KVVRVTFTSETPDYYDHLMDVDQKLLVDLYESATGQDVSLDDLRSGSHGIFEAANAFN
                     SRTDGPIVHLMQGSNNLRAAVILAAEATVLRKGKDGKSVSHPQTLVVCGGLGDERRHS
                     DPRIAAAVNNLVAKRFEITLQDPPGLYLDRFVTTGMTTPDGTDARTFWTVERGDPGHV
                     VRARFAAPVALGYAVGAIKLGGRRIVAGAQLAERLSVRIEAVARAASFQPKRQPCVAG
                     SGL"
     gene            4601..5545
                     /locus_tag="VEV43_06365"
     CDS             4601..5545
                     /locus_tag="VEV43_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23177.1"
                     /translation="MPFVTRFDTPGSLRDAPDGSPFYDDWHAYVAGLTARVTPGSGGG
                     EFFDPSEADFLPAAKRALVWMGFPRDLMVTTHRDSRAAAFAAGEVRAVQEEYLEWHVT
                     RSRGKITKVVFVTETPDYWRALAVRHPDVVLRLYRELVSPSVTMADLFPGGGPYDPGN
                     QWNTTRGIVHFVQSINRLDAAIGLAQNSVNSAPALDNYDVPPGDQTSADPRVQLDVGT
                     LARKGLSLTLAEPIGLYILGWDTTGWSKPDGSPVGDYWTIRRGVPGAALRVELAVPKS
                     EGFVLGDIRIGGRPIRTGGEIAEHVTVMALGIAGKRRG"
     gene            5542..7227
                     /locus_tag="VEV43_06370"
     CDS             5542..7227
                     /locus_tag="VEV43_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HYP23178.1"
                     /translation="MKKRAGEVLQGLDATGRVLLKGVKIPSTGSDFGVELAGAPAEPV
                     SGPKARDDIQGNVIPGFNKDHQHFLFLRIGKVPPAKEWLRSLGPRISSMDDVLGFVRA
                     HRALRLKLGVKEPGLKATWINVAFSNVAVAKLAGKEQAAAFGETSFRQGLAERSTYLG
                     DPTDPSAPGHRDNWVVGGPDNEADILLIVASDEPADLEEAVDSLREETAAAGLELLFE
                     QRGDTLPAPLRGHEHFGFKDGISQPGVRGKVSAAPGDFITPRWVDAADPHAAIYAKPG
                     QLLVWPGQFLLGEPRQNTESMTASAPPATNFPKWARRGSYVVCRRLLQDVPAFWEFVA
                     DAAAEVGLPEVRVASMLVGRWPSGAPVMRVPDADDPMLAGDEFANNHFVVDDDTRPSS
                     LRPIPDYPGDVHPPAQADALGAVCPHFAHIRKVNPRDDATDLGKPADGLLRMILRRGI
                     PFGEPIAGVKKPSSKLVAQERGLMFLCYASTIEDQFEFLTRRWLNSPVQPNLGGHDPV
                     VGQEGREPRARFVDFPGGKKPIRLDLKREWVTVTGGGYFFAPPISAIAGVLGA"
     gene            7291..7488
                     /locus_tag="VEV43_06375"
     CDS             7291..7488
                     /locus_tag="VEV43_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23179.1"
                     /translation="MRLRVGIVAALVAACSSFAVAPAQASTCQPDPLGPDFENEEELC
                     YAAMRAFCTPFVIVRKECPFR"
     gene            complement(7485..7967)
                     /locus_tag="VEV43_06380"
     CDS             complement(7485..7967)
                     /locus_tag="VEV43_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23180.1"
                     /translation="MTDYYFESKDVGEEGRTRAFSALLDVLGDQLVWAHVDGYTDYRE
                     LPTEVELAFERLKAVALDQYLERWRGPLLNRLFARLGDPLMAVRLDLAIEKHREILRV
                     YGPYSIHAEVYRKGDKNSVITIHDCDGIGFDADVQIAAQVEALAGLPPGSVREVRPGS
                     "
     gene            complement(7964..8152)
                     /locus_tag="VEV43_06385"
     CDS             complement(7964..8152)
                     /locus_tag="VEV43_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23181.1"
                     /translation="MGLEGFERPFESAGRAAAFVFSAVATSTAVPLDAWLVGTDAIAR
                     DEVFCRPVGTVTVAIARS"
     gene            complement(8143..8382)
                     /locus_tag="VEV43_06390"
     CDS             complement(8143..8382)
                     /locus_tag="VEV43_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23182.1"
                     /translation="MLKHVAAAVLAAVTTIVVVTGVFRILPASVPTAVPFVGAVLAGA
                     ALWWMPDVPRSVPAGIVAGAVVYAGFVYYVLLSWG"
     gene            8482..9018
                     /locus_tag="VEV43_06395"
     CDS             8482..9018
                     /locus_tag="VEV43_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23183.1"
                     /translation="MQRRIAIVGSFSTGKTTLAEILARRLELPLLPEVARAIVDLGFQ
                     LDKHATVEAESLIFLRQYNNELATDSFVGDRSLVDVMAYAGWVLENQPWRKEMELWEE
                     CKKLAERRLRTNYTHVYYLPIEFPIVLDDLRPDDPDFQKEIDDRILMLLRHHGVAFRT
                     ITGSVEERLDQIAKDLAG"
     gene            9258..>10089
                     /locus_tag="VEV43_06400"
     CDS             9258..>10089
                     /locus_tag="VEV43_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23184.1"
                     /translation="MLLCVTVGASLVQAAPGDAAGAAPRITGITKLSSTGGSKSAVVE
                     VPNKVWIDSSTVDARKDGPNSDVSISGKGAFVGVAFVNRSTDEAVTMRNLLIVGRWDV
                     CDGGGCPREHVYYVQGNASARRVVLLPGTYDVHLIGGPGPIEITLRFHGGTGGATKVV
                     ADEPSPVDLKAPPAEVTRTDQGAYVASSGSEFDTGRVGFSVSLMYVRAERPLDSLSGG
                     VCQINGPTAPPRHLFGPQCFALTRAGFGPGHYSIAESVRGREYTLMPLFSYAENDMGQ
                     M"
BASE COUNT         1607 a         3498 c         3512 g         1472 t
ORIGIN      
        1 actcgcctct cggcgagcgc ctgccggagg cggtgtggat cgagccgatc cccgacgcgc
       61 agctgatgcc ggcgggaagc gaccccgccg cggccgccga gctccgtgag tcgatccggc
      121 tcgcgttcgt cgccgcgctg cagcacctcg ctcccaagca gcgcgcggtg ctgatcctca
      181 gggacgtcct cggctggagg gcgagcgagg tcgccgagct cctcggcacg accgtcgccg
      241 gcgtgaacag cgcgctccag cgtgcgcgcg cgacgctcgc gaacgcggag gtgcactcgg
      301 gcagcgcgcc ggacgctctc gacgaccgca ccgagaccct gctcgcgctc tacgtcgacg
      361 cgttcgagcg ctacgacatg gaccggctga cgtcgctgct gcacgacgac gcgacgtgga
      421 cgatgccgcc ctacgagctg tggctcaaca cgcacgagga catccggagg tggtgcctcg
      481 ggcgaggcat cgggtgccgc gggtcccgcc tcgtgccggt gagggcgaac gcctcgccgg
      541 ccttcgccca gtacaagccc gccgcggacg gcggcttcga cgcgtggtcg ctgcaggtgc
      601 tcgacgtcgc cggccgcaag atccgcggca tctgcttctt ccttgacgcc gagtggctct
      661 tcccgatgtt cgggctgccc ccgcggcttc ccgcggaacc gctgacgatg cgtccccgtc
      721 tcgtgcggac agatgagctt tcgcgcgacg gacggtccaa ccccacgacg acccgctgaa
      781 ggaaggacta cggagatgag gacgatcctt gggatctggg cgcaccccga cgacgaggtg
      841 ttcacgtccg gcgggctgat ggcggacgcg gtccggcgcg gtgatcgcgt cgtgtgcctg
      901 cacctcacgc gcggcgaggc gggcctctac caccgccagg tgtggccggc gcgcgaccta
      961 ggagccgttc ggacggccga gctcgaggcc tcgctcgccc gcctcggcgt caccgagcag
     1021 cggttcctcg acctccccga cggcgggctc tgcctggtcg cgtgggacga ggtcgtcgcc
     1081 cggctgcacg acgagctcgt cgacgtcgac cccgacgtga tcgtcacgtt cgggagcgac
     1141 gggttcaccg gccaccccga ccaccgcgcg ctgtcgagct gggtctcgac ggccgtccgt
     1201 ttgtgggaga agccgagcgc gcgcgtgctg caggcagtcg tgccgcgggc gtgggagcgg
     1261 accgccgtcc accgcctcaa cgagttcgac ttcttctggc cgggctatcc cgacttcgcc
     1321 gggccggcgg acgcgggcat ccggctcgac gacgagctcg tcgaagcgaa ggtcgacgcg
     1381 gtgaaggcgc acgcgagcca gatggattgt ctgttcgagg cgtacggcga cgacttcttc
     1441 cgttcgatcc tgtcgacgga ggtctaccgg accggcgtgc ggcccgcgtt ccgctcgagg
     1501 acgctgcagg acgcgaggcg tccgtaagat cggcgagtgg cttacgacga ggacctggcc
     1561 gaccgcatcc gggagctcgc tgccggcgag cgcgcgctga ccgagaagaa gatgttcggc
     1621 gggctcgcgt tcctcgtccg cgggcacatg gcggtcgcgg cgagcgggca gggcgggatc
     1681 ctcgtgcgcg tcgacccggc cgagtccgag gccctgctgg cgaagaccga cgcctatccg
     1741 atggagatgc gcgggcgcga gatgcggggg tggctgcgcc tcgacgccga gcacgtccgg
     1801 acgaaggcgc agctgtcgag gtgggtgcgc cgcgggctcg attacgcccg ctcgctgcct
     1861 ccgaagtgac atcgcggaca cctggccgaa cctttcgtcc cgattcgttg caactgtctt
     1921 tacgcctcgc cagcgcgcgc gttacgttga gcccggacct ggtcgggtag agcgctagga
     1981 ggcggcgatg gcccctgtga ccgcgaaggc aggcgacgag acctgcatgg tcttcaacac
     2041 gatcaagggg gcgccgcagg gccgcgtcgt caggttcgtc gtcggcgagg acaagcagcc
     2101 gcgcgagatg acccaggccg agacggaggc cgagctcggc gaccccttcg ccacgctgct
     2161 gctcaacaag ggcgtcttcc cgagcaccgc cgccgagctc ctcgacgccg tcgacgcccg
     2221 ggtcggcgcg aaccaccccc tgggcccgag cagccacatg tcgttcgtcc tcggcgaggg
     2281 gagccagatc ccgctcgccg acgcgacgcc ttcgaccaac gcgggcctgc gcttcctcgt
     2341 gagccgcggc tccggcccga aggggcccga cctgatcatc agcacccccg ggcccgacat
     2401 cgcgttcgtc gaggtcatgg cgtgggacaa gaagcgcaag ggcttcaact actaccggtc
     2461 ggccggcccc aagcggtggg cgttcgccgg caactcgcgc cacgcgctca ctcccccgac
     2521 gaagggcaag ggcccgttcg agtcgcatcc cagcggcaac ctgatcatga aggagctccg
     2581 gctcccctgg gtgcactggc actcgttcga ggtcgacatc ttcgaagacg ctttcccgaa
     2641 gggcgactcg cggcgcaagc acccgtggtt cacgcagaag ctcggcgcgg agatctgcga
     2701 gaccgcggtc gtcatgccga gcatcacgcg ctggaccgag gcgcgactca accaggtcgt
     2761 cgccgccaac gggaccgtga ccgatcccgc tcgcgtagtc gagcagatcg tgacgacccc
     2821 gaccgtgaac ctcgcgagca gcagccggga gagcaagggt gcgggcccgc tcgacctccc
     2881 tcccggcttc ttcgtcgact tcgacgggct cagtcgcatc gggcttcccg ggccacccgg
     2941 cttctccgtc tcccgagcga tctacgccaa gagcctgaag acgttcgact tcaggctcga
     3001 cgacggcgac gggttcgtcc agcccggtga cacgcacttc gctttcgtcg tgccggagag
     3061 ggccttcgag gacaccgaga cgctcgctca ggcggtcgac cgggggctga taacgaggcg
     3121 gctcgctgcc gcgctgctga tggtcgactt cccgaacccg gtgttctcgg cgcggcgtgc
     3181 ttcgttgctc aagcacgcgc cgaagaccgc cacgatcaag agcggcaaga gcacctactc
     3241 cgacgatatg gcgaacgcga tcatcgcggc ggccgcggcc acaccggcca atagccccga
     3301 gaaggagttc gtcgaccgct ggaacaaggc cgcgacgctg cccggccccc tcggtgacga
     3361 gctcgaggcg tactacgacg cgatcgccaa gaagctgaag acgcaggcgg ggttcgacga
     3421 cttcgtccgg ctcgccgagt cgcagcggaa acgcgtgcgg gaccagacgc ggctggtcga
     3481 gtccctgctg ctgttcccga cgacggacgt cgacgacgac gagcggcgcg cgatgaagcg
     3541 ggacgcgacc gtcgtgctcg tgagcgccta gaggggagga ccccgtgccg ctgaagaggt
     3601 tcgccgtccc gggcaacctg aaggagctgt cgaaggaggg gctgaaggcg tggagcgcgc
     3661 gcctctcgga gctgttcgac gccgtcgcgg aaccgcccgg acggcacttc tacaacccga
     3721 cgaagtccga cacgccgccc gacgcgagcg tggcggcggt gacgtggcca gccgcccccg
     3781 gaaggctgct gagccagcgc ctcgcccccg agcgccgctg ggagatcgcc gacggggacc
     3841 gcaatcagca ggacgagtac tgcgagtgga gcgtcctgcg cgacgggaac aaggtcgtgc
     3901 gcgtcacgtt cacgagcgag acccccgact actacgacca cctgatggac gtcgaccaga
     3961 agctcctcgt cgacctctac gagagcgcga ccggccagga cgtgtcgctc gacgacctgc
     4021 ggtcggggtc gcacggcatc ttcgaggccg cgaacgcgtt caactcgcgg accgacggcc
     4081 cgatcgtcca tctcatgcag ggcagcaaca acctccgtgc ggccgtgatc ctcgccgcgg
     4141 aggccaccgt cttgcgcaag ggcaaggacg gcaagtcggt ctcgcatccg cagacgctgg
     4201 tcgtgtgcgg cgggctcggc gacgagcgca ggcacagcga cccgcgcatc gcggccgccg
     4261 tgaacaacct cgtcgcgaag cggttcgaga tcacgttgca ggacccgccg gggctctacc
     4321 tcgaccgctt cgtgacgacc ggcatgacga ccccggacgg caccgatgcg cggacgttct
     4381 ggacggtcga gcgcggcgac cccgggcacg tcgtgcgagc ccgtttcgcg gccccggtgg
     4441 cgctcggcta cgccgtcggc gccatcaagc tcggcgggag gcggatcgtc gcgggggccc
     4501 agctcgccga gcgcctcagc gtccgcatcg aggcggtcgc tcgtgccgcg agcttccagc
     4561 cgaagcgcca gccctgcgta gcgggatcgg gtctctagcg atgccgttcg tcacgcgctt
     4621 cgacaccccg ggctcgttgc gcgacgctcc cgacgggtcg cccttctacg acgactggca
     4681 cgcgtacgtg gccgggctga cggcgcgggt gacccccggg agcggcggcg gcgagttctt
     4741 cgaccccagc gaggccgact tcctgccggc ggcgaagcgc gcgctcgtgt ggatgggctt
     4801 tccccgcgac ctcatggtca cgacgcatag ggactcgcgg gcggcggcgt tcgccgccgg
     4861 cgaggtgcgc gcggtccagg aggagtacct ggaatggcac gtcaccagga gccgcggcaa
     4921 gatcacgaaa gtcgtcttcg tcaccgagac gcccgactac tggcgggccc tcgcggtccg
     4981 gcaccctgac gtcgtcctcc ggctctaccg cgagctcgtg agcccgtcgg tgacgatggc
     5041 cgacctcttc cccggcggcg gcccctacga ccccggcaac cagtggaaca ccacccgcgg
     5101 catcgtccac ttcgtccagt cgatcaaccg gctcgacgcc gcgatcgggc tcgcacagaa
     5161 cagcgtgaac agcgcgcccg cattggacaa ctacgacgtc ccgcccggcg accagacgtc
     5221 cgccgacccc cgcgtccagc tcgacgtcgg cacgctcgcg cgcaaggggt tgtccttgac
     5281 gctcgcagag cccatcggcc tctacatcct cgggtgggac acgaccggct ggagcaagcc
     5341 cgacggctcg ccggtcggcg actactggac aatcaggcgc ggggttccgg gggccgcgct
     5401 gcgcgtcgag ctcgccgtcc cgaagtccga gggcttcgtc ctcggcgaca tccgcatcgg
     5461 cgggcggccc atccgcacgg ggggcgagat cgccgagcac gtaacggtga tggcactcgg
     5521 gatcgcggga aagaggcgcg gatgaagaag cgggcgggag aggttctgca agggctcgac
     5581 gcgaccggtc gcgtgctgct gaagggcgtc aagatcccga gcacgggaag cgacttcggc
     5641 gtcgagctcg ccggtgcgcc ggccgagccc gtgtccgggc cgaaggcgcg cgacgacatc
     5701 cagggcaacg tcatcccggg attcaacaag gaccatcagc acttcctctt cctgaggatc
     5761 ggcaaggtcc cgcccgcgaa ggaatggctg cggtcgctgg ggccgcgcat ctcgtcgatg
     5821 gacgacgtgc tcggtttcgt cagggcccac cgggcgctgc gcttgaagct cggcgtgaag
     5881 gagccgggcc tcaaggcgac gtggatcaac gtcgcgttct cgaatgtggc ggtcgcgaag
     5941 ctcgccggca aggagcaagc ggccgcgttc ggcgagacga gcttccggca gggcctggcc
     6001 gagcgttcga cctatctcgg cgatccgacg gacccgagtg ctcccggcca tcgggacaac
     6061 tgggtcgtag gggggccgga caacgaggcc gacatcctgt tgatcgtcgc gagcgacgag
     6121 cccgcggacc tcgaggaggc cgtcgactcg ttgagggaag agacggcggc cgcgggactc
     6181 gagctcctgt tcgagcagcg aggcgacacg ctccccgccc ccttgcgcgg ccacgagcac
     6241 ttcgggttca aggacggcat ctcccagccc ggcgtgcgcg gcaaggtgtc ggcggcaccg
     6301 ggcgacttca tcacgccgcg ctgggtcgac gcggccgacc cgcatgcggc gatctacgcc
     6361 aagccggggc agctcctcgt gtggccgggt cagttcctgc tgggtgagcc gcgccagaac
     6421 accgagagca tgacggcgtc cgcgcctccc gcgacgaact tcccgaagtg ggcgcggcgc
     6481 gggtcgtacg tcgtgtgccg gcggctgctc caggacgtgc ccgcgttctg ggagttcgtc
     6541 gccgacgccg ccgccgaagt gggtcttccg gaggtacggg tcgcttcgat gctcgtcggc
     6601 cggtggccga gcggtgcgcc ggtgatgagg gtgccggacg cggacgaccc gatgctggcc
     6661 ggagacgagt tcgccaacaa ccacttcgtg gtcgacgacg acacgcggcc ctcgagcctc
     6721 cgcccgatcc cggactaccc cggcgacgtc catccgcccg cccaggcgga cgcgctcggc
     6781 gcggtctgcc cgcacttcgc gcacatcagg aaggtcaacc cgcgtgacga cgcgaccgac
     6841 ctcggcaagc ccgccgacgg cctgctgcgg atgatcctgc ggcgcgggat cccgttcggc
     6901 gagccgatcg cgggcgtgaa gaagccgtcg tccaagctcg tcgcgcagga gcgcgggctg
     6961 atgttcctct gctacgcgag cacgatcgag gaccagttcg agttcctcac gcgccgctgg
     7021 ctcaactcgc cggtccagcc gaacctcggc gggcacgacc ccgtcgtcgg gcaggagggc
     7081 cgcgagccgc gggcccggtt cgtcgacttc ccggggggga agaagccgat ccgcctcgac
     7141 ctgaagcgcg agtgggtgac ggtcaccggc ggcggctact tcttcgctcc gccgatcagc
     7201 gcgatcgccg gggtcctggg ggcctaggag gattctccgg accccacagc caagagtccg
     7261 ccgacaatcg gatcgacgga ggtgggatgg atgcgtctca gagtcgggat cgtcgcggcc
     7321 ctcgtggcgg catgcagctc gttcgcggtg gcgccggcgc aggcttcgac gtgccagccc
     7381 gacccgctgg gcccggactt cgagaacgag gaagagctct gctacgcggc gatgcgcgcc
     7441 ttctgcacgc cgttcgtgat cgtgcggaag gagtgcccgt tccgttagga gcctgggcgg
     7501 acctcccgga ccgagccggg cgggagcccc gccagagcct cgacctgcgc agcgatctgg
     7561 acgtccgcgt cgaacccgat cccgtcgcaa tcgtggatcg tgatcacgga gttcttgtcg
     7621 cccttgcggt agacctcggc atggatcgag taggggccgt agacgcggag gatctcgcga
     7681 tgtttctcga tcgcgagatc gagccggacg gccatcagag gatcgcccag ccttgcgaac
     7741 agacggttga gcagaggacc gcgccagcgt tccaggtatt gatcgagggc gacggccttc
     7801 aggcgttcga acgcgagctc gacttccgtc ggaagctcgc gatagtccgt gtacccgtcg
     7861 acgtgggccc agacgagttg gtcgccgaga acgtcgagga gggccgagaa cgcgcgcgtc
     7921 ctgccctcct cgccgacgtc cttggactcg aagtagtaat ccgtcacgac cgcgcgatcg
     7981 ctacggtcac ggtccccact ggccggcaga agacctcgtc ccgtgcgatc gcgtcggtcc
     8041 cgacgagcca agcgtcgagc ggcactgctg tcgaggtcgc cacggcggag aacacgaacg
     8101 ccgcggcccg ccccgccgac tcgaacggac gctcgaatcc ctctagcccc acgagaggag
     8161 aacgtagtag acgaaaccgg cgtagacgac ggcgccggcg acgatcccgg ccggtaccga
     8221 gcgggggacg tcgggcatcc accacagcgc cgcgccggcg agcacggccc ccacgaacgg
     8281 gacggccgtg ggaaccgatg cgggcaggat gcggaacacg cccgtgacga cgacgatggt
     8341 agtgacggcg gcgaggacgg ccgccgcgac gtgcttcagc accgagcgct ccgatcgacg
     8401 ccgtggatca tcgcaccatg atcctctctt cttccgtccg tccggcgcgc gtgccggccc
     8461 ggtcgtggga gagttgcggc cgtgcagcgg cggatcgcga tcgtcggcag cttctccacc
     8521 ggcaagacca cacttgccga gatcctcgcg cgccggctcg agctcccgct cctccccgag
     8581 gtcgcccgcg ccatcgtcga cctcggcttc cagctcgaca agcacgctac cgtcgaagcc
     8641 gagagcctca tcttcctccg ccagtacaac aacgagctgg cgacggactc gttcgtcggc
     8701 gaccgctcgc tcgtcgacgt catggcctac gcgggctggg tcctcgagaa ccagccgtgg
     8761 cgcaaggaga tggagctctg ggaggagtgc aagaagctcg ccgagcgccg gctccgcacg
     8821 aactacaccc acgtctacta cctgccgatc gagttcccga tcgtgctcga cgacctccgg
     8881 cccgacgacc cagacttcca gaaggagatc gacgaccgga tcctgatgct cctacggcat
     8941 cacggcgtcg cgttccggac gatcacgggc tcggtcgaag aacgcctcga ccagatcgcg
     9001 aaggacctcg cgggctaggg gccgcagggc tccccgcggg cccaagaccg tgccgccggc
     9061 cggatatgag cgggcgagca ggagccatac gacttctgga gaaggtgcat ctcaaggcgt
     9121 ggagagggaa gcgcgtgcat ccttcaaagg gcctgacggc agccgtgacg caactgcgtt
     9181 gttgggttct gcgtcattgc ggacgacccc gagccctgag ctgcagaagc gagaaggcat
     9241 tgtcccgatt taggtcgttg ctgctctgtg tcaccgttgg cgcgtcgtta gtccaggcgg
     9301 cgccgggcga cgccgcgggc gcggcccctc gcataacggg catcaccaag ctctcgtcca
     9361 ccggcgggtc gaagtccgca gtcgtcgagg tcccgaacaa ggtgtggatc gactcgtcta
     9421 cggtcgacgc gcgcaaggat gggccgaact cagacgtcag catctcaggg aaaggcgcct
     9481 ttgtaggggt cgcgttcgtc aaccggtcca ccgacgaagc ggtgaccatg cgaaacctct
     9541 tgatcgtcgg tcgttgggac gtgtgcgacg gaggcgggtg tccccgagag cacgtgtact
     9601 acgtgcaagg gaatgcttcc gctcgccgcg tcgtgctcct ccctgggacg tacgacgtgc
     9661 atctcatagg tggcccaggg ccgatcgaga taaccctcag gttccatggc ggcaccggcg
     9721 gggccacgaa ggtcgtggcg gacgaaccat cccccgtcga tctcaaagct ccgcccgccg
     9781 aggtgaccag aaccgaccag ggtgcttacg tcgccagctc gggatcggag ttcgacacgg
     9841 ggcgggtcgg gttctccgtc tcgttgatgt acgtgcgcgc cgaacgcccg ctcgactcac
     9901 ttagcggcgg agtgtgccag atcaacgggc cgactgcccc gccgcgtcat ctcttcgggc
     9961 cacagtgctt cgcgttgacc cgcgctggct tcgggccggg gcactacagc attgccgaat
    10021 cggtccgtgg ccgcgagtac acgttgatgc cgctcttctc ctacgccgag aacgatatgg
    10081 gacagatgc
//
LOCUS       DASQDO010000221         8064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_450565, whole genome shotgun sequence.
ACCESSION   DASQDO010000221 DASQDO010000000
VERSION     DASQDO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450565"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VEV43_06405"
     CDS             <1..1324
                     /locus_tag="VEV43_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP23185.1"
                     /translation="VPGGAVATADADPRWAGAIVPPGDPGMQTACVCALREAFEEVGL
                     LLADGPAIPRESSHDGARFLEACLAAGTRLRADRLVPGGRWVTPSGSPVRFDALFFLT
                     EAPPGWVPEPDPGEVESVRWATPAGALADLASGAALMAPPTIEMLQRLEGLPDVAAAL
                     GALTDEPVGNQRILSTRVSPLVHVVLAPNPGLMTGPGTNTYVVGSSPAIVVDPAVADD
                     DYIGEVARVAGEVAAIVVTHRHSDHTGGVGALREALRAEVPVRAWGDDPVGGAAPEPL
                     VDGEVLTAGGSSLRVVHTPGHASDHACLYAERTATLFSGDTILGEGTAVIAPPDGDMG
                     DYLRSLGRLRELHVERIFPGHFRALDGGRDVIDGYVAHRRARERRILDALTPGPSTIE
                     EIVERAYDDTPRELHPAAAMSALAHLELLEDEGRARSQDDRWRLGGVD"
     gene            1383..3461
                     /locus_tag="VEV43_06410"
     CDS             1383..3461
                     /locus_tag="VEV43_06410"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYP23186.1"
                     /translation="MRKGSWQWAVRVAQLAAWGTLALVTVGLFAAGLDTPDFRLGLCL
                     LAALGAWVFFFFHEALPRWGSHRPTAWIGTLVTVGFTCAFYALLRGEIPSAQLIFVPV
                     IVVTSLLGFVPEAVLTGVLATAGYWAVSNAAGPAPRPLALAFNSGVFLLSGVVAGLLA
                     RELRAHYRGEQQEHRLATAVRYRLLAVLNSIDEAIVFSDRQGTVRVVNKRAAELFQIE
                     PDAQLGEPVVQLLRTIARKTEEPEDFMELFQELRDDPELELREELEQILPVRRQLKLY
                     SGPAFDDAGDLVGRIDVYTDISENVRRAAEVKELYEQARNTAESYQRGLLPKAVPKLP
                     RLSMVAHYVAAAGKRAVCGDFYDFMSLPDGRVAVVMGDVCGVGPAAANDAALARYTLR
                     SYAPENADPGELLRRVNERTSAHLPSDRFIRLMYGALDPERAVLDYANAGHVPPIVYR
                     HKSGEVEWLGEGGLVLGIEEDARYKSGHVELEPGDMLVMYTDGVTEAPRFGQPFGQGR
                     FMDLIKDWGMGTPGELVQAIRRAVEAWTQEGELRDDLALLVCQVVPDTAVDELVRELV
                     LPNEPSRIGEIRSFVSAFLVDLRAPVEASSEILLAVGEAVANATRHGRRAEGRSEVRI
                     RCSAENRSVSVTIADDGGGFDMSRLERTELPDPFASGGRGLFLMRELMDEVKFESSDR
                     GTNVVLTRRV"
     gene            3489..3686
                     /locus_tag="VEV43_06415"
     CDS             3489..3686
                     /locus_tag="VEV43_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23187.1"
                     /translation="MDLRTWTLATRIRYAVVMTATNALVVTGIAALAGSLSRSSLGAA
                     IPFGAIAFVVHGWFWYPRAKR"
     gene            3738..4907
                     /locus_tag="VEV43_06420"
     CDS             3738..4907
                     /locus_tag="VEV43_06420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYP23188.1"
                     /translation="MAREAVIVEAVRTPLGKKNGALKDWHPVDLLAEVLRETVDRSGV
                     DAAKVDDVICGCVSQVGEQSLNVGRNGWLAAGLPEEVPATTIDRQCGSSQQAAHFAAQ
                     GVISGAYDVAIACGVESMTRVPMGSSAQTGGWPFSEKMMARYEGGLVPQGISSELIAE
                     KWGLAREQLDAFGARSHVKAGEATEAGAFKNEILPIKVTTDDGQVLFDRDQGIRTPNM
                     EKIAALTPSFKPDGVTTAANSSQITDGAAALLITTPETAKELGLRPRARFVSFALAGT
                     DPITMLTGPIPATRKALDKAGLSLEDVDLVEINEAFASVVLAWKKELGIADDWFDERV
                     NVHGGAIALGHPLGASGARLMTTLVNALEQRDARYGLQTMCEGGGLANATVIERL"
     gene            4918..6201
                     /gene="serS"
                     /locus_tag="VEV43_06425"
     CDS             4918..6201
                     /gene="serS"
                     /locus_tag="VEV43_06425"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYP23189.1"
                     /translation="MLDIQLIRTEHDAVLDGLAKRMERVEAEAALSQILSLDERRRAL
                     IADVEAERKRRNEEAQRFAAAKRAGEDVPPPTNAGAAKQRLAEMEGELRGVENDLDDL
                     MAGLPTVPAPEVVAGGKEANEVVKTWGEPPEIERPRDHVELATALGLVDFERGVKLGG
                     SGFWLYRGLGARLEWALVTWFVRQHLDAGYHFMLPPHLLLGSAGFASGQFPKFYDDVY
                     HLANADSKEGQFLLPTSETAILGAFQNETIDAADLPMKLFAYTPCYRKEKAGTHSDER
                     GTVRGHQFNKVEIFQLVGEDGAAAALDEMVGHAERLVEGLGLHYRTSLLAAGDASAAM
                     RKTLDVEVWMPSTGKYKEVSSVSWAGDYQARRARIRYKPKGEKGRFVHTLNGSALATS
                     RIFPAILEQYQQEDGSVRVPEVLQDVVGTDVLGGP"
     gene            complement(6198..6527)
                     /locus_tag="VEV43_06430"
     CDS             complement(6198..6527)
                     /locus_tag="VEV43_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23190.1"
                     /translation="MRRIRPVPALVVLALIFVLGRPFGYVRAAVALAMAAAIFWLGFS
                     YFRSAGEVPPDPETEDVSDGSLRYVCTMCGLELKVEVAASDKAPSHCREKMVLVEGRG
                     RPPLKPV"
     gene            complement(6586..6816)
                     /locus_tag="VEV43_06435"
     CDS             complement(6586..6816)
                     /locus_tag="VEV43_06435"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HYP23191.1"
                     /translation="MPKSKSKRVTRQPPPKTKPKHSPLWVGALFFTLLLTGVAIIICN
                     YVGAFPPENQAWVLWYGLGLISGGFVVATQWH"
     gene            6855..7517
                     /locus_tag="VEV43_06440"
     CDS             6855..7517
                     /locus_tag="VEV43_06440"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HYP23192.1"
                     /translation="MRWLHVAGKFLISVGVGFLLFVLWTLYGTGIYTAQQQRQLAEEF
                     DRLPAFERQSPEGNPLPPGPPPGYDPAPGAPVFRMHLPTIEDKPMMVVEGVDTEELKL
                     GPGHYPSCRAGFERPLCTAWDEIFPGEKGRVIVSGHRTTYGQPFWALDEMEKGDPIHF
                     ETRWGDFTYRVTDKQIVPADSQAIVVSSKKYEVVLTTCNPKYSAAERLIVFAELETEA
                     AR"
     gene            complement(7627..7827)
                     /locus_tag="VEV43_06445"
     CDS             complement(7627..7827)
                     /locus_tag="VEV43_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23193.1"
                     /translation="MAERKYTYQLIGSLIVGLLIVIVTISIVTGKLGPRLDRREELRE
                     LEDVREERREQREEQREERFDN"
     gene            complement(7830..>8064)
                     /locus_tag="VEV43_06450"
     CDS             complement(7830..>8064)
                     /locus_tag="VEV43_06450"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HYP23194.1"
                     /translation="SYFGYEDGVEHAAGEETGRATLHVIGGTLVVAVLAVKVVAVRWW
                     RDLGRYLPLIGMTLFGFLALTWATSAGDYVFGS"
BASE COUNT         1211 a         2877 c         2805 g         1171 t
ORIGIN      
        1 ggtccccggc ggcgcggtcg cgaccgcgga cgcggacccg cgctgggccg gggctatcgt
       61 cccgccgggc gaccccggga tgcagaccgc ctgcgtctgc gcgctgcggg aggcgttcga
      121 ggaggtcggc ctcctgctcg ccgacggacc ggcgatcccg cgcgagagct cgcacgacgg
      181 agcccgcttc ctcgaggcgt gcctcgcggc cgggacccgg ctccgtgcgg accgcctcgt
      241 ccccggcggc cgctgggtga cgccgtccgg ctccccggtc cgcttcgacg cgctgttctt
      301 cctgaccgag gccccgcccg gctgggtccc cgagcccgac cccggcgagg tggaatccgt
      361 gcggtgggcg acgccggccg gggccctcgc ggacctcgcc tcgggggccg cgctgatggc
      421 cccgccgacg atcgagatgc tccagcggct cgagggcctg cccgacgtcg cggccgcgct
      481 cggcgcgctc acggacgagc ccgtcggcaa ccagcggatc ctgtcgaccc gggtctcgcc
      541 gctcgttcac gtagtcctcg cgccgaaccc cggcttgatg accggcccgg gcaccaacac
      601 ctacgtcgtc ggctcgagcc cggcgatcgt cgtcgacccc gccgtcgcgg acgacgacta
      661 cataggagag gtcgcccggg tcgcgggaga ggtcgctgcg atcgtcgtca cccaccgcca
      721 ttcggaccac accggcggcg tgggggcgct gcgcgaagcc ctccgggccg aggtcccggt
      781 ccgggcctgg ggcgacgacc cggtcggagg agccgctccc gagccgctcg tcgacggcga
      841 ggtcctcacg gccggaggct cgtctctgcg ggtcgtccac acgccgggcc acgcttcgga
      901 ccacgcctgc ctctacgcgg aacgaacggc gacgctgttc tccggcgaca cgatcctcgg
      961 cgaggggacc gcggtcatcg cgccgccgga cggtgacatg ggcgactacc tgcggtcgct
     1021 cggccggctg cgcgagctgc acgtcgagcg gatcttcccg ggccacttcc gcgcgctgga
     1081 cgggggtcgc gacgtgatcg acggctacgt cgcgcaccgc cgggcccgcg aacgacggat
     1141 tctcgacgcc ctgacgcccg gcccctcgac gatcgaggag atcgtggaac gcgcctacga
     1201 cgacacgccc cgcgagctcc atcccgccgc ggcgatgtcg gcgctggcgc acctggagct
     1261 gctcgaggac gagggccgcg cgagatcgca ggatgaccgc tggcgcctgg gcggcgttga
     1321 ttagggtagg aaccgagccc ccactcgagt cgaggcagtt gatctccacg gattcccgag
     1381 acgtgcggaa gggcagctgg cagtgggccg tgcgcgtcgc gcagcttgcc gcctggggca
     1441 cgctcgccct cgtcacggtc gggttgttcg ccgccggcct cgacaccccc gacttccggc
     1501 tcggcctctg cctcctcgcc gccctcgggg cgtgggtctt cttcttcttc cacgaggcgc
     1561 ttccccggtg gggatcccac cggcccaccg cgtggatcgg gacgctggtc accgtcgggt
     1621 tcacgtgcgc gttctacgcg ttgctgcgcg gcgagatccc gtccgcgcag ctgatcttcg
     1681 taccggtgat cgtcgtcacg tcccttctgg gattcgtccc cgaggccgtg ctcacgggcg
     1741 tcctcgcgac cgcggggtac tgggcggtct cgaacgcagc gggcccggcc cctcgccccc
     1801 tggcgctcgc cttcaactcc ggcgtgttcc tgctctcggg tgtcgtcgcc ggcctgctcg
     1861 cccgcgagct ccgggcccac taccgcggcg agcagcagga gcaccgcctc gcgaccgcgg
     1921 tccggtaccg gctgctcgcg gtcctgaact cgatcgacga ggccatcgtg ttctcggacc
     1981 gccaggggac cgtgcgcgtc gtgaacaaga gggcggccga gctcttccag atcgagcccg
     2041 acgctcagct cggcgagccc gtcgtccagc tgctgcgcac gatcgcgcgc aagaccgagg
     2101 agcccgagga cttcatggag ctcttccagg agctgcgtga cgaccccgag ctcgagctgc
     2161 gcgaggagct cgagcagatc ctcccggtgc gacgccagct caagctctac tcgggcccgg
     2221 ccttcgacga cgcgggcgac ctcgtcgggc ggatcgacgt ctacacggac atctccgaga
     2281 acgtccgccg cgcggccgag gtcaaggagc tctacgagca ggcgcgcaac acggccgaga
     2341 gctaccagcg cggcctcctg ccgaaggcgg tgccgaagct cccccggctc agcatggtgg
     2401 cgcactacgt cgccgcggcc ggcaagcgcg cggtgtgcgg ggacttctac gacttcatgt
     2461 cgttgcccga cgggcgcgtc gccgtggtga tgggagacgt gtgcggcgtg ggccccgcgg
     2521 ccgcgaacga cgcggcgctg gcgcgctaca cgctgcgctc ctacgcgccg gagaacgcgg
     2581 acccgggcga gctcctgcgg cgcgtcaacg agcgcacgag cgcgcacctc ccgtcggatc
     2641 gcttcatccg cctgatgtac ggcgcgctcg atcccgagcg tgcggtgctc gactacgcga
     2701 acgcagggca cgtcccgccg atcgtctacc ggcacaagtc cggcgaggtc gagtggctcg
     2761 gagaaggggg cctggtcctc ggcatcgagg aggacgcgcg ctacaagtcg ggccacgtcg
     2821 agctcgagcc cggcgacatg ctcgtgatgt acaccgacgg cgtgaccgag gcgccgcgtt
     2881 tcgggcagcc gttcgggcag ggtcgcttca tggacctgat caaggactgg ggcatgggga
     2941 cgccgggcga gctcgtccag gcgatccggc gcgcggtcga ggcgtggacg caggagggag
     3001 agctgcgcga cgacctcgcg ctgctcgtgt gccaggtcgt gcccgacacc gcggtcgatg
     3061 agctcgtgcg cgagctcgtg ctcccgaacg agccgtcgcg catcggcgag atccgctcgt
     3121 tcgtgtcggc gttcctcgtc gacctccggg cccccgtgga agcgtcgtcc gagatcctgc
     3181 tcgccgtcgg agaggccgtc gcgaacgcga cccgccacgg ccgccgcgcc gagggccgca
     3241 gcgaggtccg catccgctgc tcggccgaga accgctcggt gtcggtgacg atcgcggacg
     3301 acggcggggg cttcgacatg tcgcgactgg aacggacgga gctgccggac ccgttcgcct
     3361 cggggggccg cgggcttttc ctgatgcgcg agctgatgga cgaggtgaag ttcgagtcct
     3421 cggaccgggg gacgaacgtg gtgctgaccc gccgggtcta acgtcgctcg tacagcgaag
     3481 gaggaccgat ggacctgcgg acttggacac ttgcgacacg gatccggtac gcggtggtca
     3541 tgaccgcgac caacgctctc gtcgtcacgg gcatagctgc cctcgccggt tcgctctcgc
     3601 ggagctctct cggggcggcg atcccgttcg gggccatcgc gttcgtggtc catggctggt
     3661 tctggtaccc gcgcgccaag aggtagctca tagcgcgata cttgacctcc cggtcaagaa
     3721 aaaaaaagga ggtccccgtg gcccgcgaag ccgtcatcgt cgaagccgtc cgcacgcccc
     3781 tcggcaagaa gaacggcgcc ctcaaggact ggcatcccgt cgacctcctc gccgaggtcc
     3841 tccgggagac cgtcgaccgc tcgggcgtcg acgccgccaa ggtcgacgac gtcatctgcg
     3901 gctgcgtctc gcaggtcggc gagcagtccc tgaacgtcgg gcgcaacggc tggctcgccg
     3961 ccggcctccc cgaggaggtc cccgccacca ccatcgaccg ccagtgcggc tcgtcccagc
     4021 aggccgcgca cttcgccgcg cagggcgtca tcagcggcgc gtacgacgtc gcgatcgcgt
     4081 gcggcgtcga gagcatgacc cgcgtcccga tggggagcag cgcgcagacc ggcgggtggc
     4141 ccttctccga gaagatgatg gcgcgctacg aggggggtct cgtcccccag ggcatctcgt
     4201 ccgagctcat cgccgagaag tggggcctcg cgcgcgagca gctcgacgcg ttcggcgcgc
     4261 gctcgcacgt caaggccggc gaggccaccg aggccggggc cttcaagaac gagatcctcc
     4321 cgatcaaggt gaccaccgac gacggccagg tcctgttcga ccgcgaccag ggcatccgca
     4381 ctccgaacat ggagaagatc gcggccctca cgccgtcgtt caagcccgac ggcgtgacga
     4441 ccgccgcgaa ctcgtcgcag ataaccgacg gcgctgccgc cctgctgatc acgaccccgg
     4501 agacggcgaa ggagctcggg ctccgtcccc gggcgcgctt cgtctcgttc gcgctcgccg
     4561 gcaccgaccc gatcacgatg ctgaccgggc cgatccccgc gacgcgcaag gcgctcgaca
     4621 aggccggcct ctccctggag gacgtcgacc tcgtcgagat caacgaggcg ttcgccagcg
     4681 tcgtcctcgc gtggaagaag gagctcggca tcgcggacga ctggttcgac gagcgcgtca
     4741 acgtccacgg cggcgcgatc gccctaggcc acccgctcgg cgcgtcgggg gcgcggctga
     4801 tgacgacgct cgtcaacgcc ctcgagcagc gcgacgcgcg ctacggcctc cagacgatgt
     4861 gcgaaggcgg gggcctcgcg aacgcgaccg tgatcgagag gctctagcct tagcgacttg
     4921 ctcgacatac aactgatccg aaccgaacac gacgccgtcc tcgacggcct cgcgaagcgc
     4981 atggagcgcg tcgaggccga agctgcgctg tcgcagatcc tgtcgctcga cgagcgccgg
     5041 cgcgcgctga tcgcagacgt cgaggccgag cgcaagcggc gcaacgagga agcccagcgg
     5101 ttcgccgcgg ccaagcgcgc gggcgaggac gtgccgcctc cgacgaacgc gggggctgcg
     5161 aagcagcggc tcgccgagat ggagggcgag ctccgcggcg tcgagaacga cctcgacgac
     5221 ctcatggccg ggctcccgac cgtcccggcc cccgaggtcg tcgccggcgg caaggaggcg
     5281 aacgaggtcg tcaagacctg gggcgagcct ccggagatcg agagaccccg cgaccacgtc
     5341 gagctcgcga ccgcgctggg cctcgtcgac ttcgagcgcg gcgtgaagct cggcggctcc
     5401 ggcttctggc tctacagagg tctcggcgcg cggctcgagt gggcgctcgt cacctggttc
     5461 gtccggcagc acctcgacgc gggctaccac ttcatgctcc cgccgcacct gctgctcggc
     5521 tcggccggct tcgcgtcggg acagttcccg aagttctacg acgacgtcta ccacctcgcg
     5581 aacgcggact cgaaggaagg ccagttcctg ctgccgacgt cggagaccgc gatcctgggc
     5641 gcgttccaga acgagacgat cgacgcggcg gaccttccga tgaagctctt cgcgtacacg
     5701 ccgtgctacc gcaaggagaa ggccgggacg cactccgacg agcgggggac cgtgcgcggc
     5761 caccagttca acaaggtcga gatcttccag ctcgtcggag aggacggcgc ggcggcggct
     5821 ctagacgaga tggtcgggca cgccgagcga ctcgtcgagg ggctcggcct ccactaccgc
     5881 acgtcgctgc tcgccgccgg ggacgcgagc gcggcgatgc gcaagacgct cgacgtcgag
     5941 gtgtggatgc cgagcaccgg caagtacaag gaggtctcgt cggtgtcgtg ggccggcgac
     6001 taccaggccc gccgggcgag gatccgttac aagcccaagg gcgagaaggg ccgctttgtc
     6061 cacacgctca acggttccgc gctcgcgacc agccggatct tcccggcgat cctcgagcag
     6121 taccagcagg aggacgggtc cgtcagggtc ccggaggtcc tgcaggacgt cgtcgggacc
     6181 gacgtcctcg gcggtcccta gaccggcttc agagggggcc gcccgcggcc ctcgaccaga
     6241 accatcttct cccggcagtg cgacggcgcc ttgtccgacg ccgcaacctc taccttcagc
     6301 tcgaggccgc acatggtgca cacgtagcgg agcgagccgt cgctcacgtc ctcggtctcg
     6361 gggtccgggg ggacctcacc ggcgctgcgg aagtagctga acccgagcca gaagatggca
     6421 gccgccatcg cgagcgccac ggccgcccgg acgtagccga acggccgccc caggacgaat
     6481 atcagggcga gcaccacgag ggccgggacc gggcggatcc gcctcatctc ggttctccac
     6541 agcggaatcc acagacgtgg ataaacgaca tccttgtgat tcgggtcagt gccactgcgt
     6601 tgctacgacg aacccacccg agatcagccc gagcccgtac cacagcaccc acgcctggtt
     6661 ctccgggggg aaagccccga cgtagttgca gatgatgatg gccacgccgg tcagcagcag
     6721 cgtgaagaac agcgccccca cccacagcgg cgagtgcttc ggcttggtct tcggcggcgg
     6781 ctggcgcgtg acgcgcttcg acttgctctt cggcatagat cgagaatggt aatcggttga
     6841 ccgatacttc cttcatgagg tggttgcacg tcgccggcaa gttcctgatc tcggtggggg
     6901 tcgggttcct cctgttcgtc ctctggacgc tctacgggac cgggatctac acggcccagc
     6961 agcagcgtca gctcgcggaa gagttcgacc gcctcccggc cttcgagcgc cagagccccg
     7021 aggggaaccc gctgccgccc ggccctccgc cgggctacga cccggccccc ggcgcgcccg
     7081 tgttccgcat gcacctgccg acgatcgagg acaagccgat gatggtcgtc gagggcgtgg
     7141 acaccgagga gctcaagctc ggccccggcc actacccctc gtgtcgggcc ggcttcgagc
     7201 gccccctgtg cactgcatgg gacgagatct tcccgggcga gaagggccgc gtgatcgtgt
     7261 ccgggcaccg gacgacgtac ggccagccgt tctgggcgct cgacgagatg gagaaaggcg
     7321 accccatcca cttcgagacc cgctggggcg acttcaccta ccgcgtgacc gacaagcaga
     7381 tcgtgccagc ggactcccag gcgatcgtcg tctcgtcgaa gaagtacgag gtcgtgctca
     7441 cgacgtgcaa cccgaagtac tccgcggccg aacggctgat cgtcttcgcg gagctcgaga
     7501 ccgaggcggc ccgatgaagc gttcgtggaa gtcgaagctg ctcgagctgt tggtgtgggc
     7561 cgcggtggcg gtcgggaccg cggtcgtcct cgtgatgctg tcggagaagc tgctgcccgc
     7621 gaacttctag ttgtcgaacc gttcttcgcg ttgctcttcc cgttgctcgc ggcgttcctc
     7681 tcgcacgtct tcgagctcgc gcagctcttc ccggcggtcg aggcgggggc cgagcttgcc
     7741 cgtgacgatc gagatcgtga cgatcacgat cagcaggccg acgatcagcg agccgatcag
     7801 ctggtacgtg tacttgcgtt cggccatcgt cagctcccga agacgtagtc gccggccgac
     7861 gtcgcccacg tgagcgcgag gaagccgaac agcgtcatcc cgatcagcgg gaggtagcgg
     7921 ccgaggtcgc gccaccaccg aaccgcgacg accttcacgg cgagcacggc gacgacgagc
     7981 gtgccgccga tcacgtggag ggtggcccgg cccgtctcct cccccgcggc gtgctcgacc
     8041 ccgtcctcgt agccgaagta cgag
//
LOCUS       DASQDO010000222         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_634857, whole genome shotgun sequence.
ACCESSION   DASQDO010000222 DASQDO010000000
VERSION     DASQDO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634857"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VEV43_06455"
     CDS             <1..1293
                     /locus_tag="VEV43_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008748566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HYP23195.1"
                     /translation="VVDIGPGAGEHGGDVVYTGPVSGLVRNRKSVTGDYLAGRKSIEV
                     PERRRAAKGEIVVRGAHQHNLRDIDVTFPLGCFVAVTGVSGSGKSTLVNEVLYRAVHQ
                     KLNRNARLVAGRHKAIEGLDQVDKVIDIDQSPIGRTPRSNAATYTGVFDHIRKLFAKT
                     QDAKRRGYQPGRFSFNVRGGRCEACAGDGTIKIEMHFLPDVYVPCEVCKGKRYNRETL
                     EVRFKGKNIAEILEMSVAEAAEFFSAIPPIARHMETLRDVGLGYVRLGQPAPTLSGGE
                     AQRVKLSTELAKRSTGKTLYILDEPTTGLHYDDVAKLLGVLHRLVDAGNSVVVIEHNL
                     DVIKTADHIVDLGPEGGAGGGSIVVAGTPEEVAACPDSYTGRFLQPLLEPRPAPAKKA
                     AARKTAAASKEGRRVGTARRAGRPPAPAQTTYFSVMSE"
     gene            1308..2129
                     /locus_tag="VEV43_06460"
     CDS             1308..2129
                     /locus_tag="VEV43_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23196.1"
                     /translation="MRAEGVLQAFPRARAALPKGRALPARLWATRHRAVLGLLWLHVP
                     ALAELAAVRGYSPLHSLLEISIVAAAALLATWHGLDRPARAAVATFGLFTCSALLVHF
                     SGGLTEMHFHFFVMVVVVALYQDWFPFLVGIAFVLLHHGVIGTLAPQDVYSSATAVEN
                     PWGWAAVHAGFILAESVACLAAWKKSEQYAAQIAEQAVRRREALELNDQVVQGLVVAK
                     ASFELGDVEGGRKALDATLGRAKDFVSDLLGQDHTTGTAQPGSLVREKPATVRPK"
     gene            2126..2509
                     /locus_tag="VEV43_06465"
     CDS             2126..2509
                     /locus_tag="VEV43_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP23197.1"
                     /translation="MKTEKCTVVLADDTPEIRTLLRLTLEDQGDIEVVGEAGDGAEAV
                     KIVSELQPNALVLDLAMPVMDGFEAIPEVRRRAPETKIIVLSGFDTGLMSERALSLGA
                     DMYFEKGISFFDLAEKVVDTCPGRA"
BASE COUNT          403 a          879 c          902 g          370 t
ORIGIN      
        1 gtcgtcgaca tcgggcccgg cgcgggcgag cacggcggcg acgtcgtcta cacgggcccg
       61 gtctccggcc tcgtccgcaa ccgcaagtcg gtgaccggcg actacctcgc cgggcgcaag
      121 tcgatcgagg tgcccgaacg acggcgcgcc gcgaagggcg agatcgtcgt gcgcggcgcg
      181 caccagcaca acctccggga catcgacgtg acgttcccgc tcggctgctt cgtcgccgtc
      241 acgggcgtgt cgggatcggg caagtcgacg ctcgtgaacg aggtgctcta ccgggcggtg
      301 caccagaagc tcaaccggaa cgcccgcctc gtcgcgggac ggcacaaggc gatcgagggc
      361 ctcgaccagg tcgacaaggt catcgacatc gaccagtcgc cgatcggccg gaccccccgc
      421 tcgaacgccg cgacgtacac cggtgtcttc gaccacatcc gcaagctgtt cgccaagacg
      481 caggacgcga agcggcgcgg ctaccagccc gggaggttct cgttcaacgt ccgcggcggg
      541 cgctgcgagg cgtgcgccgg cgacgggacg atcaagatcg agatgcactt cctgcccgac
      601 gtctacgtcc cgtgcgaggt gtgcaagggc aagcgctaca accgcgagac gctcgaggtg
      661 aggttcaagg gcaagaacat cgccgagatc ctcgagatga gcgtcgccga ggcggccgag
      721 ttcttctccg ccatcccgcc catcgcccgc cacatggaga cgctgcgcga cgtcggcctc
      781 ggctacgtcc ggctcgggca gccggcgccg acgctgtcgg gcggcgaggc gcagcgggtg
      841 aagctctcga ccgagctcgc gaagcgctcg acgggcaaga cgctgtacat cctcgacgag
      901 ccgacgaccg gtctccacta cgacgacgtt gcgaagctcc tcggcgtgct gcatcgcctc
      961 gtcgacgcgg ggaactccgt cgtcgtgatc gagcacaacc tcgacgtgat caagaccgcc
     1021 gaccacatcg tggacctggg ccccgaggga ggggccggcg ggggatcgat cgtcgtcgct
     1081 gggacgcccg aggaggtggc ggcgtgcccg gactcgtaca cggggcgctt cctgcagccg
     1141 ctgctcgagc cgcgcccggc ccccgcgaag aaggccgccg cgaggaagac cgcggctgcc
     1201 tccaaggagg gccgccgcgt cggcacggcc cgccgcgccg gcagaccgcc ggcacctgcg
     1261 caaacaacgt atttctccgt aatgtccgaa taacagatag tcagtccatg agggctgagg
     1321 gggttctcca ggcgtttcca cgcgcgaggg ccgcgctccc gaaagggcgc gccctgccgg
     1381 cgcggctgtg ggcgacgcgc catcgcgccg tcctcggcct gctgtggctg cacgtcccgg
     1441 cactggccga gctcgcggcc gtccggggct actcgccgct ccattccttg ctcgagatct
     1501 cgatcgtcgc ggcggccgct ctgctcgcga cgtggcacgg cctcgaccgg ccggcgcgcg
     1561 ccgcggtggc cacgttcggc ttgttcacct gctcggcgct gctcgtgcac ttctccggtg
     1621 ggctcaccga gatgcacttc cacttcttcg tgatggtggt cgtcgtcgcg ctctaccagg
     1681 actggttccc gttcctcgta ggtatcgcgt tcgtgctgct ccaccacggc gtgatcggga
     1741 cgctcgcgcc gcaggacgtc tacagcagcg ccaccgcggt ggagaacccg tgggggtggg
     1801 ccgccgtgca cgcgggattc atcctggccg agagcgtggc ttgcctggcc gcctggaaga
     1861 agagcgagca gtacgccgcg cagatcgcgg agcaggccgt ccggcgccgg gaggcgctcg
     1921 agctgaacga ccaggtcgtc caggggctcg tcgtggcgaa ggcctcgttc gagctcggcg
     1981 acgtggaagg gggccggaag gcgctcgacg ccacgctcgg ccgtgcgaag gacttcgtct
     2041 ccgacctgct cggccaggac cacacgacgg gcaccgcaca gccgggctcc ttggtgcgcg
     2101 agaagcctgc gacggtgaga ccgaagtgaa gaccgagaag tgcaccgtcg tgctcgcgga
     2161 cgacacgccg gagatcagga cgctgctgcg cctcacgctc gaggaccaag gagacatcga
     2221 ggtggtcggc gaggccggtg acggcgcgga ggccgtgaag atcgtgagcg agctccagcc
     2281 caatgcgctc gtgctcgacc tcgcgatgcc ggtgatggac ggcttcgagg ccatccccga
     2341 ggtacgccgg cgcgctcccg agacgaagat catcgtgctg tccggcttcg acacgggcct
     2401 gatgtccgag cgcgccctgt cgctgggggc cgacatgtac ttcgagaagg gcatcagctt
     2461 cttcgacctg gcggagaagg tagtggacac ctgccccggc cgggcctgac gcggagcggt
     2521 ggggaaccca ggcttcgaat tcagtgcgca tgcc
//
LOCUS       DASQDO010000223         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_940342, whole genome shotgun sequence.
ACCESSION   DASQDO010000223 DASQDO010000000
VERSION     DASQDO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940342"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VEV43_06470"
     CDS             <1..1385
                     /locus_tag="VEV43_06470"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP23198.1"
                     /translation="FYWSEEMSQAGAFTREGTIDNPSPLGVGVTRTEFTATCPATPST
                     QGVDGYLFTLPDVPLRDAVADFTATNGAALRVATWTGDCTLVDEATGPTHVLGAGVRY
                     VLVSTDSGFDTAVTMEVTPAPSRIVNLGQDLTRTGTGTTRDVNNGGLAVGVWRRAAFT
                     WSRAGGYAELPFLPGAYWDQAEAMAVNDAGVIVGWGTDDEGFKKPVRWTGSGIEELPV
                     LPGGRYGVANDVSSSGVIVGEADGVTDRGLRTRPVIWHPDGTIEEIPLHPLDPPLDMG
                     FAEGVNDRGTVIGWDMSSIQGDGRQVAWVRSADGVKTNLNDLIDPASGWDLRVPLDVN
                     EREQIVGIGILTIGGVTYEGRAFLLTPTTPPVDPEKLETLLDVAVEGRGQTTTLTARL
                     TEAASGAPVGDRSIAFYSGGELLGSRATDATGVARIELPPGHRGASRTFEAVFAGDDL
                     YGASSARSGR"
     gene            1416..1583
                     /locus_tag="VEV43_06475"
     CDS             1416..1583
                     /locus_tag="VEV43_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23199.1"
                     /translation="MLLGSRGADTLYGRGGPDVLDGGLGDDRLVAGKGRDKCRAGGGR
                     DDFSSCERVER"
     gene            complement(1598..>2063)
                     /locus_tag="VEV43_06480"
     CDS             complement(1598..>2063)
                     /locus_tag="VEV43_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23200.1"
                     /translation="VPPLNSLHVAAPLPWDAMVGRRVYGADNREWFVGRRWLPWKPRR
                     RRGSADALDVADGMHVGIDISDGPVSIILGILVVGVVILLFPVIITALEVVLVLVLLP
                     LFVVARLILRKPWIIVARTKGPHPEERTAVVSGWRASREAMEAMIQDIRSNG"
BASE COUNT          318 a          706 c          757 g          282 t
ORIGIN      
        1 cgttctactg gtcggaggag atgtcgcagg ccggggcttt cacacgcgag ggcacgatcg
       61 acaacccgag cccgctcggc gtcggagtca cccgcacgga gttcaccgcg acctgcccgg
      121 cgaccccgtc gacccagggc gtcgacggct acctgttcac gctgcccgac gtcccgctga
      181 gagacgcggt cgcggacttc accgctacga acggcgcggc gctgcgcgtc gcgacgtgga
      241 cgggggactg cactctcgtg gacgaggcga cggggccgac gcacgtcctc ggcgccggcg
      301 tccgctacgt cctcgtctcc accgactccg gcttcgacac cgcggtcacg atggaggtga
      361 cccccgcgcc gtcgcggatc gtgaacctcg gccaggacct cacgcggacc gggaccggca
      421 cgacgaggga cgtcaacaac ggcggccttg cggtcggcgt gtggcgcagg gccgcgttca
      481 cctggagtcg cgccggcggg tatgcggagc ttccgttcct ccccggcgcg tactgggacc
      541 aggccgaggc gatggcggtc aacgacgccg gcgtgatcgt cgggtggggg accgacgacg
      601 aaggcttcaa gaagcccgtg cgctggaccg gctccggcat cgaggagctt cccgtcctgc
      661 ccggcggtcg ctacggcgtc gcgaatgacg tgagctcgag cggggtgatc gtcggtgagg
      721 ccgacggcgt cacggatcgc ggcctccgca cgcgtccggt gatctggcat cccgacggga
      781 cgatcgagga gatcccgctg catccgctcg acccgccgct cgacatgggc ttcgcggagg
      841 gcgtcaacga ccgcggcacg gtcatcggct gggacatgag cagcatccag ggtgacggtc
      901 gtcaggtcgc gtgggtccgg agcgcggacg gcgtgaagac gaacctcaac gacttgatcg
      961 accccgcgtc ggggtgggac ctgcgcgtgc ccctcgacgt gaacgagcgc gagcagatcg
     1021 tcggcatcgg catcctcacg ataggtggcg tgacctacga gggccgcgcg ttccttctga
     1081 ctccgacgac acctccggtc gacccggaga agctcgagac gctgctcgac gtcgccgtcg
     1141 agggccgggg gcagacgacg acgctgaccg cgcgtctgac ggaggccgcg tccggcgccc
     1201 cggtcgggga ccgctcgatc gcgttctact cgggcggcga gctgctgggc tcccgtgcta
     1261 ccgacgcgac cggggttgcg cggatcgagc tcccgcccgg ccaccgcggc gcgagccgaa
     1321 cgttcgaggc ggtgttcgcc ggcgacgatc tctacggagc atcgtcggcg cgatcggggc
     1381 ggtagccgca ggagtaagca agcagcttct tcgacctgtt gctggggagc cgcggggccg
     1441 acacgctgta cgggcgcggc gggcccgacg tcctggacgg agggttaggc gacgatcgcc
     1501 tggtcgcggg aaagggccgg gacaagtgcc gggccggtgg gggccgcgac gacttcagca
     1561 gctgcgaacg ggtggagcgg tagccggccc ccgcctctca gccgttcgag cggatgtcct
     1621 ggatcatcgc ctccatggcc tccctgctgg cgcgccagcc cgacacgacc gccgtgcgtt
     1681 cctccggatg cggccccttg gtgcgggcga cgatgatcca cggcttgcgc aggatgagcc
     1741 gggcgacgac gaacagcggg aggaggacga ggacgagcac gacctcgagg gccgtgatga
     1801 tgaccgggaa cagaaggatg acgacgccga cgacgaggat ccccaggatg atgctcaccg
     1861 gcccgtccga gatgtcgatg ccgacgtgca tgccgtcggc gacgtcgagg gcatcggccg
     1921 agccgcgcct ccgccggggc ttccacggca gccagcgccg cccgacgaac cactcccggt
     1981 tgtcggcccc gtagaccctc cgccccacca tcgcgtccca gggtaacggc gcggccacat
     2041 gtaaagaatt gagtggaggc acc
//
LOCUS       DASQDO010000224         6402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_695757, whole genome shotgun sequence.
ACCESSION   DASQDO010000224 DASQDO010000000
VERSION     DASQDO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695757"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..876
                     /locus_tag="VEV43_06485"
     CDS             190..876
                     /locus_tag="VEV43_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HYP23201.1"
                     /translation="MFGRTRDEARHEDRRVTGHEEVAARGGVSPGASLTGVVVAFGAM
                     FVLTAVISGILVGLGVEDELTPGQATDVGIATGIGLVVAQFLSYMWGGYAAGRMSRGA
                     GSVNGALVALTALLVGVAIGAIATWVGATEGLRTPFNSSQLPADGEVLRWGAGLAIAA
                     VAAMFFGGVVGGAFGSRWHTKLERRTVTEADDRDRHRVNEAVDTRRRNDAETIDLRED
                     RTVTTRSSRE"
     gene            1074..1520
                     /locus_tag="VEV43_06490"
     CDS             1074..1520
                     /locus_tag="VEV43_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23202.1"
                     /translation="MRKLPLLLAAASLALLPSAEAAAPGTLPQLPPLAAEEKPLFAGG
                     GTPLTNGIFFPGTALCVGDDCQGVPYEIARGTDIRFYNLDNSAVANNHRVVSKKTTKK
                     GRPLFQSDTIGGPESTLIKTSHLKPGVYPFYCSVHFGMEGILEITK"
     gene            complement(1527..2441)
                     /gene="thrB"
                     /locus_tag="VEV43_06495"
     CDS             complement(1527..2441)
                     /gene="thrB"
                     /locus_tag="VEV43_06495"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HYP23203.1"
                     /translation="MPASVKVRVPATVANLGPGYDSLGAAIRMHLQIEIEPRRDSVEI
                     VVEGEGADQLPADDTNLVIRSMNTFFDHVGRRPSGFAVKVTNPIPLGAGLGSSAAAIV
                     GGLFAARAVTGRTVPQTEMIQLATDLEGHADNVMPALIGGLVVCYRGEDEDDIRYFRV
                     EPSDRLVPMLAVPREGFPTKTARKALPAEVKFADAQFTASRAALLVAAMSAGAGADVL
                     ADAMNDRLHEPHRLKLMPETAAVYEEIQEAGLPVALAGAGPSLLVVVPRPESATRAEQ
                     VRRICRERRSGWRVFVSEWEPEGAKAHP"
     gene            complement(2525..3595)
                     /gene="thrC"
                     /locus_tag="VEV43_06500"
     CDS             complement(2525..3595)
                     /gene="thrC"
                     /locus_tag="VEV43_06500"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYP23204.1"
                     /translation="MTAEAAVADRRGIIRRWFDHLPVDESTPIVTLGEGATPLIHSER
                     LSEETGAEVWLKYEGLNPTGSFKDRGMTLAISKALEEGSSAVVCASTGNTSASAAAYA
                     GRAGMTCAVLIPSGNVALGKLAQALVHGARVLEVDGNFDQALTLTREMAERWPITLVN
                     SVNPFRIEGQKTGAFEVVESLGRAPDLHLVPVGNAGNITAYWRGYREARDAGWSESLP
                     RMHGFQAEGANPIVRGEPVADPVTIATAIRIGNPASWKEAEDAAGTSGGAIASVRDKE
                     ILEAYRDLAREGVFVELASAASVAGLRSMHSRGEVPKGAVVTCVLTGHGLKDPDWAIA
                     GAPVPKKVPPRIEPILAELDLA"
     gene            complement(3625..4935)
                     /locus_tag="VEV43_06505"
     CDS             complement(3625..4935)
                     /locus_tag="VEV43_06505"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HYP23205.1"
                     /translation="MAPAPLERPVRIGLLGCGTVGGATASILLRHASDLEQRTGVPVE
                     LRRVAVRDASRERGVELPEGSITMDPWAVVKDPAIDVVVETIGGLDPARELVLAALAA
                     GKHVVTANKELIATHGRELLDAAASGGVDVLFEAAVGGGIPVIRSIKESLAGDRLVRV
                     MGIVNGTTNFILTRMSEDAMSFEAALAEAQGLGYAEADPTADVEGHDAASKLAILATL
                     AFGGRVVASDVRRQGIANVTAEDVAAAHDLGYEVKLLAVAERDEGQVAVRVHPAMVPK
                     THPLASVREAFNAVFVEGEEVGELMLLGRGAGGAATASAVVGDVVELARNLAQGAPGA
                     PTDAFSRDATIRPPEEARVRYYLVLSVLDQSGVLAAVAGVFARHDVSIASVRQEGSGD
                     EATLVLITHTSTEGQHQATFDDLAGLDAVKDVRSTIRVEGAPER"
     gene            5033..>6402
                     /locus_tag="VEV43_06510"
     CDS             5033..>6402
                     /locus_tag="VEV43_06510"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYP23206.1"
                     /translation="MRTRTTRRSKVAAALVALAAVAATQLAVDAAAPQDGQKGSGGRA
                     VAHRAGGRVVTASKAPDGRIYRVADAAIEPTLGITKEGAIFYTAAASVTGVDVLRSTD
                     DGGTWENTSPRLPTGDKSHPITLDPYIYVAEATGRIFNIDLTVACSNLSFSDDDGETW
                     TSNPLACGRPVNDHQTLFSGPPAISPTVTYPEVVDYCWNDFGAGSSCSKSLDGGITWV
                     STGSPAFSGVAAGGEETGADVCGGFHGHGVVGPDGTVYLPKEYCGQPWIGISKDEGAT
                     WTRVQVANNTTERLGSDTSVAVDAKGNLYYTYESADQRLWLVTSKDGGEHWTNPKMVG
                     SPGVEEVIYPTIDVGDPGKIAIAYMGTEESPFAKCDPDCTNDDYAGVRWNGYITMTTN
                     ALAKDPLFYSGTVNAKNDPLYIGRCDFSNRCDPILDFIDVEIAPDGTPWGAFVDPCGE
                     ACKASGV"
BASE COUNT          962 a         2338 c         2191 g          911 t
ORIGIN      
        1 ctcccccggc cgccagctcg tcgagcgccg actcgaccac cggcggatag tgctcgccgt
       61 cgaccacggc taggtaacgg atggctgcct cctcgatggc tccggcggcg gggttggaag
      121 gaaaaggtta cggagagtct ccaagtggga accctatgtg acacaacccc cgcgactttg
      181 gaggtcacga tgttcggtcg cactagagac gaagcccgcc acgaggaccg ccgagtcacc
      241 ggtcacgagg aggtcgccgc ccgcgggggt gtctcccccg gcgcgagtct gacgggggtc
      301 gtcgtcgcgt tcggcgcgat gttcgtgctc accgccgtga tctccgggat cctcgtcggc
      361 ctcggcgtcg aagacgagct gacgcccggc caggccaccg acgtcgggat cgccaccggg
      421 atcggcctcg tcgtcgccca gttcctctct tacatgtggg gcggctacgc agcagggcgc
      481 atgagcaggg gggccggctc cgtcaacggg gcgctcgtcg ccctcacggc cctgctggtc
      541 ggcgtcgcca taggtgccat cgccacgtgg gtgggggcta cggaaggcct gaggacgccg
      601 ttcaactcga gccagctccc ggcggacggc gaagtgctgc gctggggtgc gggcctcgcc
      661 atcgccgccg tcgccgcgat gttcttcggc ggcgtggtcg ggggtgcgtt cggctcccgc
      721 tggcacacga agctcgagcg ccgaacggtc accgaggcgg acgatcgcga ccgccaccgg
      781 gtgaacgagg cggtcgacac ccgccggcgg aacgacgccg agacgatcga cctccgcgag
      841 gaccggacgg ttacgacccg gtcgtcgagg gaataacgag cagcagcgat tccgaacggg
      901 tacggagacg caaccgcacc ggggacgcca gtgccgccgg ccctcgcagg gggcagcgcc
      961 ggcagaggga ggcgtccccg ctgcgcgccc gggccctgtc ggggcgcccg ccgggatttt
     1021 gcctatttgg gcacttcgca ggattgcgtc ccgggtcgcc taacctagag atcgtgagaa
     1081 agcttccgct cctcctggcc gcagcatccc tcgccttgct cccgtccgcc gaggcggcgg
     1141 ctccgggcac gctgccccag ctcccgcccc tcgccgccga ggagaagcct ctgttcgccg
     1201 gcggcggaac gccgcttacg aacggcatct tcttcccggg caccgcgctg tgcgtcggcg
     1261 acgactgcca gggggtcccc tacgagatcg cccgaggcac ggacatccgc ttctacaacc
     1321 tcgacaactc cgccgtggcg aacaaccacc gcgtcgtctc gaagaagacc accaagaagg
     1381 gccgccccct gttccagtcg gacaccatcg ggggccccga gagcacgctg atcaagacgt
     1441 cgcacctgaa gccgggcgtc taccccttct actgcagcgt gcacttcggt atggaaggca
     1501 tcctcgagat caccaagtag cagcccttac gggtgcgctt tcgccccttc cggctcccac
     1561 tcgctgacga acacgcgcca ccccgaacgt ctctccctgc agatccgccg cacctgctcc
     1621 gcgcgcgtcg cggactcggg ccggggcacg accacgagca gcgacggccc cgcgcccgcc
     1681 agagcgaccg gcaggccagc ctcctggatc tcctcgtaga cggccgcggt ctcgggcatg
     1741 agcttgaggc ggtgcggctc gtggagcctg tcgttcatcg cgtcggctaa gacgtccgca
     1801 ccggcgcccg ccgacatcgc ggcgaccagg agggccgctc gcgaagccgt gaactgcgcg
     1861 tcggcgaact tcacctcggc cggcagcgcc ttgcgcgcgg tcttcgtcgg gaagccctcg
     1921 cgcgggaccg cgagcatcgg cacgaggcga tcgctcggct cgacgcggaa gtacctgatg
     1981 tcgtcctcgt cctcgccccg gtagcagacg acgaggcccc cgatcagcgc gggcatgacg
     2041 ttgtcggcgt ggccctcgag gtcggtggcg agctgaatca tctcggtctg cgggaccgtc
     2101 cggccggtca cggcgcgagc ggcgaagagc ccacccacga tcgccgcggc cgacgagccc
     2161 agcccggccc ccagcgggat cgggttcgtc accttcaccg cgaagcccga cggacgccgg
     2221 ccgacgtggt cgaagaacgt gttcatcgag cggatgacga ggttcgtgtc gtcggcaggg
     2281 agctggtcgg cgccctctcc ttcgacgacg atctcgacgg agtcgcgccg cggctcgatc
     2341 tcgatctgga ggtgcatcct gatcgcggcg ccgagggagt cgtagcccgg gccgaggttc
     2401 gcgacggtcg cgggtacccg gaccttcacg gaagcaggca tctgctcagc ctttcgtttc
     2461 ggtggaggcc ggccgaggcc gcccttcgtc gccgtcgccg gttcgcctta caggacgccc
     2521 gggatcatgc cagatcgagc tcggcgagaa tcggctcgat cctcggcggg accttcttgg
     2581 ggacgggcgc gccggcgatg gcccagtccg ggtccttcag cccgtgcccc gtcagcacgc
     2641 acgtgacgac cgcgcccttc ggcacctcgc cgcgcgagtg catggaccgc aggccggcga
     2701 ccgacgccgc ggaggcgagc tcgacgaaca cgccctcgcg cgccaggtcg cggtacgcct
     2761 cgaggatctc cttgtcgcgc accgacgcga tcgcgccgcc ggacgtcccc gcggcatcct
     2821 cggcctcctt ccacgacgcc gggttgccta tgcggatcgc cgtcgcgatc gtgaccgggt
     2881 cggcgacggg ctcgccccgc acgatcgggt tcgcgccctc tgcctggaag ccgtgcatcc
     2941 ggggcagcga ctcggaccag cccgcgtcgc gcgcctcgcg gtagccgcgc cagtaggcgg
     3001 tgatgttccc ggcgttgccg accggcacca ggtggaggtc cggggcgcgg ccgagcgact
     3061 cgacgacctc gaacgcaccc gtcttctgac cctcgatccg gaacgggttg acggagttga
     3121 cgagcgttat cggccagcgt tccgccatct cgcgggtcag cgtgagcgcc tgatcgaagt
     3181 tgccgtcgac ctcgaggaca cgggcgccgt ggacgagcgc ctgcgcgagc ttgcccaggg
     3241 cgacgttgcc gctggggatc agcaccgcgc acgtcatccc ggctcggccc gcatacgcgg
     3301 ccgccgacgc gctggtgttc ccggtcgacg cgcacacgac ggcggacgag ccctcctcga
     3361 gggccttcga gatcgcgagc gtcatcccgc ggtccttgaa cgaccccgtc gggttcagcc
     3421 cctcgtactt cagccagacc tcggcccccg tctcctccga caatcgctcg gagtggatca
     3481 gaggcgtcgc gccctcgccg agcgtcacga tcggcgtcga ctcgtcgacc gggaggtggt
     3541 cgaaccaccg gcggatgatc ccgcggcggt ccgcgacggc tgcttcagcg gtcatctgtc
     3601 ctggccgctc gccgaggcga ccggtcatct ttcgggcgct ccttcgaccc ggatcgtcga
     3661 gcggacgtcc ttcacggcgt cgaggccggc gaggtcgtcg aacgtcgcct ggtgctgccc
     3721 ttcggtggag gtgtgcgtga tcagcacgag cgtcgcctcg tcaccggaac cctcctggcg
     3781 cacgctcgcg atcgagacgt cgtgccgggc gaacaccccg gcgaccgcgg cgaggacgcc
     3841 ggactggtcg agcacggaca ggacgaggta gtagcggacg cgcgcttcct cgggcgggcg
     3901 gatcgtcgcg tctctcgaga acgcgtcggt cggagcgccg ggcgcgccct gcgcgaggtt
     3961 ccgggcgagc tcgacgacgt ctccgacgac cgcggacgcg gtagccgctc ccccggcccc
     4021 cctcccgagc agcatcagct cgccgacttc ctcaccctcg acgaagacgg cgttgaacgc
     4081 ctcacgcaca gacgccagcg ggtgggtctt cggcaccatc gccggatgca cccggaccgc
     4141 gacctgtccc tcgtcgcgct cggcgacggc gagtagcttg acctcgtagc cgaggtcgtg
     4201 ggccgcggct acgtcctcgg ccgtcacgtt cgcgatcccc tgccgccgga cgtccgacgc
     4261 gaccacgcgg cccccgaacg cgagagtggc caggatcgcg agcttggagg ccgcgtcgtg
     4321 cccctcgacg tccgcggtcg ggtcggcctc cgcgtacccg aggccctgcg cctccgcgag
     4381 cgcggcctcg aacgacatcg cgtcctcgct catccgcgtg aggatgaagt tcgtcgtccc
     4441 gttcacgatg cccatgacgc ggacgagccg gtcgcccgcc agcgactcct tgatcgagcg
     4501 gatgaccggg atgccgccgc cgaccgcggc ttcgaacagg acgtccacgc ccccgctcgc
     4561 ggccgcgtcg agcagctcgc gcccgtgcgt cgcgatcagc tccttgttcg cggtgaccac
     4621 gtgcttgccg gcggcgagcg cggcgagaac gagctcgcgg gccgggtcga gcccgccgat
     4681 ggtctcgacg acgacgtcga tagccgggtc cttcacgacc gcccagggat ccatagtgat
     4741 tgagccctcc ggcagctcga cgccgcgctc tctcgacgcg tcgcgcaccg ccacccggcg
     4801 cagctcgacg gggacgccgg tccgctgctc gaggtccgac gcgtgccgca gcaggatcga
     4861 cgcggtcgcg ccgccgacgg ttccgcaacc gagcaacccg atccggacgg gacgctcgag
     4921 gggtgcgggg gccatggacg caagttatcg cccctgcgcc gctgagcagg taatcggaca
     4981 cagggggcga aacgggacgg agaaccgatc cgacccccca ggaggccacg tcgtgcgcac
     5041 caggaccacc cgccgttcga aggttgcagc cgcgctggtc gcgctggccg ccgtagccgc
     5101 gacacagctg gcagtcgacg cggccgcgcc gcaggacgga cagaagggat ccgggggccg
     5161 cgccgtcgcg caccgggccg ggggccgcgt cgtcacggcg tcgaaggcac ccgacggccg
     5221 catctaccgc gtggccgacg ccgcgatcga gcccaccctc ggcatcacca aggaaggcgc
     5281 catcttctac acggcggcgg ccagcgtgac cggcgtggac gtcctgcggt ccacggacga
     5341 cggcggcacg tgggagaaca cctcgccgcg cctgccgacg ggcgacaagt cgcacccgat
     5401 cacgctcgat ccctacatct acgtcgccga ggcgaccggc cgcatcttca acatcgacct
     5461 gacggtcgcg tgctccaacc tgtcgttctc cgacgacgac ggcgagacgt ggacgtcgaa
     5521 ccccctcgcg tgcggccgtc ccgtcaacga ccaccagacg ctgttctccg gcccgcccgc
     5581 gatcagcccg accgtcacct accccgaggt cgtcgactac tgctggaacg acttcggcgc
     5641 cggctcgtcg tgctccaagt cgctcgacgg agggatcacg tgggtgtcca ccgggtcgcc
     5701 cgcgttctcg ggcgtggccg ccggcggcga ggagaccggc gccgacgtct gcggcgggtt
     5761 ccacggtcac ggcgtggtcg gtcccgacgg cacggtctac cttccgaagg agtactgcgg
     5821 tcagccgtgg atcggcatca gcaaggacga gggcgcgact tggacccggg tccaggtcgc
     5881 caacaacacg accgagcgcc tcggctccga cacctccgtc gccgtcgacg cgaagggcaa
     5941 cctctactac acgtacgaga gcgccgacca gaggctgtgg ctcgtcacgt cgaaggacgg
     6001 aggcgagcac tggacgaacc cgaagatggt gggctcgccg ggcgtcgaag aggtcatcta
     6061 cccgacgatc gacgtgggcg acccgggcaa gatcgccatc gcctacatgg gcaccgagga
     6121 atcgccgttc gcgaagtgcg acccggactg caccaacgac gactacgccg gcgtgaggtg
     6181 gaacggctac atcacgatga cgacgaacgc gctggcgaag gacccgctct tctacagcgg
     6241 caccgtcaac gcgaagaacg acccgctgta catcggccgc tgcgacttct cgaaccggtg
     6301 cgacccgatc ctcgacttca tcgacgtcga gatcgcgccg gacggcacgc cgtggggcgc
     6361 cttcgtcgac ccgtgcggcg aggcgtgcaa ggcgagtggt gt
//
LOCUS       DASQDO010000225         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_22638, whole genome shotgun sequence.
ACCESSION   DASQDO010000225 DASQDO010000000
VERSION     DASQDO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22638"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VEV43_06515"
     CDS             complement(<1..601)
                     /locus_tag="VEV43_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23207.1"
                     /translation="MRRNHVTILLVSLLATSFPVPHAAAAPSCFGRAATIVGTDDDDE
                     ITGTPGRDVIYAGKGRDEVHAGGGKDLVCGAGAIDLLYGEGGDDRLEGGPGYVDDWLF
                     GGAGADRLTPGCNGPEGDLMEGGAGPDRLIGDPCGSASMVPGPGDDFVRATRGEVTFP
                     HASGPIDADLTEGVVTGEGQDVIEVVRRDPGSDDRLVTVV"
     gene            complement(638..>1651)
                     /locus_tag="VEV43_06520"
     CDS             complement(638..>1651)
                     /locus_tag="VEV43_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP23208.1"
                     /translation="TAGSRFPTAPNDPFFEKQWGMVKIQAPEAWQLATGRGIKIAFVD
                     SGIDLDHEDFDCPGKIEVLPGSNLGGKRPDNPNDTYGHGTFVGAIAGACTNNGVGVVG
                     VAPEATLVPISVMPYFRANPIPMDKAMAHGIRFATKAGAHVINLALGENVTSHFGAQR
                     FPLTEAALTKARSKGVVIASAAGNYAQPTCEYPSMSRHVICVVATDEDDLRAAYSDAP
                     VNMDPRSDEPRMEPVVAAPGGRGATCDTNVYSAWADEGSTCAGGKYTSLFGTSASAPF
                     VAGIAALLYERLGGVRSDENADLITQTILDSADDLYGPGWDPVVGFGRVNALKAVQAL
                     PSG"
BASE COUNT          217 a          594 c          584 g          256 t
ORIGIN      
        1 cgacgacggt gacgagacgg tcgtcggagc cggggtcgcg ccggacgacc tcgatgacgt
       61 cctggccctc gccggtcacg acgccctcgg tgaggtccgc gtcgatcgga ccggacgcat
      121 gcgggaaggt gacctccccg cgggtggcgc gcacgaagtc gtcgcccggc cccgggacca
      181 tcgacgcgct cccgcacggg tccccgatca ggcggtccgg ccccgcccct ccctccatca
      241 ggtctccctc cgggccgttg cagcccggcg tcagccggtc ggctccggcc ccgccgaaga
      301 gccagtcgtc gacgtagccg ggcccccctt cgagacggtc gtcgccgccc tcgccgtaga
      361 ggaggtcgat cgcgcccgcg ccacagacga ggtctttgcc gccgccggca tggacctcgt
      421 cgcggccctt gcccgcatag atgacgtcgc gacccggggt cccggtgatc tcgtcgtcgt
      481 cgtcggtccc gacgatcgtg gcggctcggc cgaagcagct cggcgccgcg gccgcgtgcg
      541 ggacgggaaa gctcgtcgcg agcagcgata cgagcaggat ggtgacgtgg tttcgtctca
      601 tgggcttaga acgcccggcc cccgtgaatc gttccgctca gcccgagggc agcgcctgca
      661 cggccttcaa cgcgttgacg cgcccgaacc cgacgacggg atcccagccc ggcccgtaga
      721 ggtcgtcggc actgtcgagg atcgtctggg tgatcaggtc cgcgttctcg tctgagcgca
      781 ccccgccgag acgctcgtag agcagcgcgg ctatgccggc gacgaacggc gccgacgccg
      841 acgtcccgaa caggctcgtg tacttgccgc cggcgcaggt ggagccttcg tccgcccacg
      901 cggagtagac gttcgtgtcg caggtggccc cgcgtccgcc cggtgccgcg accacgggct
      961 ccatgcgcgg ctcgtccgag cgcgggtcca tgttcaccgg cgcgtcgctg taggccgcgc
     1021 gcaggtcgtc ctcgtccgtg gcgacgacgc agatgacgtg acgcgacatc gaggggtact
     1081 cacaggtggg ctgcgcgtag ttccccgcgg cggacgcgat cacgacgccc ttggaccgcg
     1141 ccttcgtcaa cgcggcctcg gtcaggggga accgctgggc gccgaagtgg ctggtcacgt
     1201 tctcgccgag ggcgaggttg atcacgtgcg cgcccgcctt cgtcgcgaac ctgatcccgt
     1261 gggccatcgc cttgtccatc gggatggggt tggcgcggaa gtacggcatg acgctgatcg
     1321 ggaccagcgt cgcctcgggc gcgacgccga ccacgcccac cccgttgttc gtgcacgcgc
     1381 ccgcgatggc accgacgaag gtcccgtggc cgtaggtgtc gttcgggttg tccggccgct
     1441 tcccgccgag gttcgagccc ggcaggacct cgatcttgcc cgggcagtcg aagtcctcgt
     1501 ggtcgaggtc gatcccggag tcgacgaacg cgatcttgat cccccggccg gttgccagct
     1561 gccacgcctc gggagcctgg atcttgacca tgccccactg cttctcgaag aaggggtcgt
     1621 tcggcgcggt ggggaagcgg gagccggccg t
//
LOCUS       DASQDO010000226         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1124405, whole genome shotgun sequence.
ACCESSION   DASQDO010000226 DASQDO010000000
VERSION     DASQDO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124405"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VEV43_06525"
     CDS             <1..255
                     /locus_tag="VEV43_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23209.1"
                     /translation="TERVRLALRSLQHRLPDGLRTRLKKVEAGVERWGAEVEELRADA
                     ARPAEEPPPDEESSALPPLLAFVVPAAVAGILLGRRSRSA"
     gene            283..975
                     /locus_tag="VEV43_06530"
     CDS             283..975
                     /locus_tag="VEV43_06530"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HYP23210.1"
                     /translation="MKWLLRALVVLVVLLVAVDFGAKLLVENLASRALSNRRGVNGSV
                     DVSFGGFPFLLALKDRRFGNVTIEADDVRSGGFMTASAPSPQSEVRMDSVRLELEDVA
                     VSGQVWRDDGDGRVSATSGGGSAVLSQSALNKMVPPEYSSKLTLRDGSVRVVANTAQF
                     GTHEAEVAEDQVRLEGDAGSGTLVVEAAAPVGAITIPLPTLIDGVVFEDIDVRRGELE
                     LMFSVRDVSLEL"
     gene            complement(970..1674)
                     /locus_tag="VEV43_06535"
     CDS             complement(970..1674)
                     /locus_tag="VEV43_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP23211.1"
                     /translation="MSALGGAVLLALALVTCEAEPRLRDDGVVPVVLVHGYGGDSSMM
                     SAIAERLREEGHEVVPVDLPLAGIGDIEGSVEVVAAAVDETRAPEVDLVGFSAGGVVA
                     RAYAGSVDEEDRPRVVVTLASPHHGTKLAAAAGELCTGACEQLAPGSAFLEELNEPDE
                     TPPGTEFVSVWSASDRVVTPPRSAVLDGAVNVRLQDLCPGSRAGHADLLRDPVVLEVL
                     LRALAGELGSVDCAAL"
     gene            complement(1685..2344)
                     /locus_tag="VEV43_06540"
     CDS             complement(1685..2344)
                     /locus_tag="VEV43_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP23212.1"
                     /translation="MTLDASASPGRPALKTLIAEDHTLVSQGLEVMLSMADGVDLVGV
                     VATGEAAVERAEREEVDVILMDVNLGKAMNGIEATRLVKERAPSTRVLILTMFTDPGT
                     VAEAVKAGADGYLSKGASKEVVVQAIKDVAEGKSVLDPNVTEGIFGRIGGRDPQALTD
                     RELTVLQELTHGKSTREVAEHIHVSEETVKTYLKQIFRKLGVHDRTEAVAEAFRRGLV
                     H"
     gene            complement(2341..>2558)
                     /locus_tag="VEV43_06545"
     CDS             complement(2341..>2558)
                     /locus_tag="VEV43_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23213.1"
                     /translation="IRVEEADGFVHIVIEDDGKGYDPATSVDDDEHHGMWLMRERAER
                     VGGRIELDTLPGRGTRVEAVLPGGVAR"
BASE COUNT          343 a          935 c          876 g          404 t
ORIGIN      
        1 accgagcgcg tccggctcgc cctccggtcg ctgcagcacc gtctgcccga cgggctccgc
       61 acccggctga agaaggtcga ggctggggtc gagcgctggg gggccgaggt cgaggagctg
      121 cgcgcggacg ccgcccgccc ggccgaagag cctcctcccg acgaggaatc gtccgcgctc
      181 ccgcctctgc tcgcgttcgt cgtccccgca gccgtggccg ggatcctcct ggggcgccgg
      241 tcgcgcagcg cttagctccc gcggcgcgta tcgtcggcag ccatgaagtg gctgctgcgc
      301 gcgctggtcg tcctcgtcgt ccttctggtc gccgtcgact tcggcgcgaa gctcctcgtc
      361 gagaacctcg cctcgcgcgc gctctcgaac cgtcgcggcg tcaacggctc ggtcgacgtg
      421 tccttcggcg ggttcccgtt cctgctcgcg ttgaaggacc gccggttcgg caacgtcacg
      481 atcgaagcgg acgacgtccg cagcggcggc ttcatgaccg cgtcggcccc ctctcctcag
      541 tccgaggtcc ggatggactc cgtgcgtctc gagctcgaag acgtcgcggt gtccggccag
      601 gtgtggcgcg acgacggcga cggacgcgtc tcggcgacgt cgggcggggg ctccgcggtc
      661 ttgtcgcaga gcgcgctgaa caagatggtt ccccctgagt actcgtcgaa gctcacgttg
      721 cgcgacggct ccgtgcgggt cgtcgcgaac acggcacagt tcggcacgca cgaggccgag
      781 gtcgccgagg accaggtccg cctcgaagga gacgcgggct cgggcacgct cgtcgtcgag
      841 gccgcggccc ccgtgggcgc cataacgatc ccgctcccga cgctgatcga cggcgtcgtg
      901 ttcgaggaca tagacgtgcg ccggggcgag ctcgagctca tgttctcggt gcgcgacgtg
      961 agcctggagc tatagcgccg cgcagtccac gctgccgagc tcgcctgcga gagcgcgcag
     1021 caacacctct aggacgacgg ggtcgcgcag caggtctgcg tgtccggccc gggagcccgg
     1081 gcagaggtcc tggagccgga cgttgaccgc cccgtcgagt accgcggacc gcggaggggt
     1141 gaccacgcga tccgatgcgc tccacaccga cacgaactcg gtccccggcg gggtctcgtc
     1201 gggctcgttg agctcttcga ggaacgccga cccgggcgcg agctgctcgc acgcacccgt
     1261 gcacagctct cccgcagccg ccgcgagctt ggtgccgtga tgcggcgacg cgagcgtcac
     1321 gaccacgcgc ggacgatctt cttcgtcgac ggagcccgcg taggcacggg cgacgacgcc
     1381 tcctgccgag aacccgacga ggtcgacctc gggggcgcgg gtctcgtcga ccgcggcggc
     1441 gacgacctcg accgagcctt cgatgtcacc gatgccggcc agaggaaggt cgaccgggac
     1501 gacctcgtgc ccctcctcgc gcagacgctc cgcgatggcc gacatcatcg acgagtcgcc
     1561 gccgtatccg tggacgagca ctaccgggac gacaccgtcg tcgcggagtc gtggctccgc
     1621 ttcgcacgtg acgagcgcga gcgcgaggag cacggcgccg cctagcgcag acacggtctc
     1681 tcgtctagtg gacgagcccg cggcggaacg cttcggcgac cgcctcggtg cggtcgtgca
     1741 cgccgagctt gcggaagatc tgcttcaggt acgtcttgac ggtctcttcc gagacgtgga
     1801 tgtgctcggc gacctcgcgc gtcgacttgc cgtgcgtgag ctcctgcagg acggtgagct
     1861 cgcggtccgt cagcgcctgt gggtcgcggc ccccgatccg cccgaagatc ccttcggtca
     1921 cgttcgggtc gagcaccgac ttgccctcgg ccacgtcctt gatcgcctgc acgacgacct
     1981 ccttggacgc gcccttcgac aggtagccgt cggcgccggc cttcaccgcc tcggccaccg
     2041 ttccggggtc ggtgaacatc gtgaggatca gcacgcgcgt cgacggcgcg cgctccttga
     2101 cgagccgcgt cgcctcgatg ccgttcatcg ccttgccgag gttgacgtcc atgaggatca
     2161 cgtcgacctc ctcgcgttcg gctctttcca ccgccgcttc gccggtcgcc acgacgccta
     2221 cgaggtcgac gccgtcggcc atcgagagca tgacctcgag gccctgcgac acgagcgtgt
     2281 ggtcctcggc gatcaacgtc ttgagcgcgg gccggccggg ggacgcgctc gcatcgagcg
     2341 tcatcgcgcg accccgcccg gcagcacggc ctcgacgcgc gtgccgcggc ccggcagcgt
     2401 gtccagctcg atgcgcccac cgacgcgttc agcccgctct ctcatcagcc acatcccgtg
     2461 atgctcgtcg tcgtcgaccg aggtggcggg gtcgtagccc ttcccgtcgt cctcgatcac
     2521 gatgtgcacg aagccgtcgg cctcctcgac ccggatca
//
LOCUS       DASQDO010000227         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_206696, whole genome shotgun sequence.
ACCESSION   DASQDO010000227 DASQDO010000000
VERSION     DASQDO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206696"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEV43_06550"
     CDS             <1..227
                     /locus_tag="VEV43_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23214.1"
                     /translation="IAGAYRGPNTAVDVALTCGVVRNGGTVARVADPLTGPVAVLGDV
                     QAVYGAGLTSCHEIKIKYLDGTVSYNDTCP"
     gene            242..622
                     /locus_tag="VEV43_06555"
     CDS             242..622
                     /locus_tag="VEV43_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23215.1"
                     /translation="MIRTSKVALVATVAAIGISVVPAAPAGAALGPCRAVKTGVGTFL
                     RPGWEEVAIAGAYTAPAGAIGVELTCSVVANDRVYASVSDEVPGPVAVLAGTGSVPTN
                     AAITTCYTLRVAYVDRSYTTYTCP"
     gene            complement(673..1947)
                     /locus_tag="VEV43_06560"
     CDS             complement(673..1947)
                     /locus_tag="VEV43_06560"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYP23216.1"
                     /translation="MEGTTSPLETPGDTAWETALAQLDEAAELMGLAPGVHVTLRNPK
                     RALIVSVPFRMDDGSTRVYQGYRVHHNVTRGPAKGGIRYHPDVGLGEVKALAMWMTWK
                     CAIAGIPFGGAKGGVAVDPRTLSRGELERMTRRYASEILPLIGPERDIPAPDMNTNEE
                     IMSWIMDTYSQNKGYSVPGVVTGKPVAIGGSKGRGGATSRGVMFMIFSTLKALGIAPE
                     EVTVAIQGYGKVGGFAAQLLHDAGCRVVAVSDVDGGLYRERGLDPEAINRHKQEAGTV
                     AGFPGADAITNDELLEIDCDVLVPAAIEGVITVKNADQVRAKIVCEAANGPVTFEGDK
                     ILNDKGIFVIPDILANSGGVTVSYFEWVQDIQAYFWSEEDVNDRLRQIMEKSFDEVYA
                     LAQAKDLTMRQAAHWIGVGRVAEAHLTRGLFP"
     gene            complement(2064..>2167)
                     /locus_tag="VEV43_06565"
     CDS             complement(2064..>2167)
                     /locus_tag="VEV43_06565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514120.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HYP23217.1"
                     /translation="EIADMLGRHVKSIDNALQRIKRKLEHHLAEAQV"
BASE COUNT          313 a          759 c          737 g          358 t
ORIGIN      
        1 ccatcgccgg cgcctacagg gggccgaaca ccgcggtcga cgtcgcgctc acgtgcggag
       61 tcgtccgcaa cggcggcacg gtcgcccggg tcgccgaccc gctcaccggc ccggtagccg
      121 tgctgggcga cgtgcaggcc gtctacggcg ccgggctgac ctcgtgccac gagatcaaga
      181 tcaagtacct ggacgggacc gtgtcctaca acgacacctg cccgtgagga gaggagcgag
      241 catgatccgc acatccaagg tcgcgctggt cgccaccgtg gcggcgatcg ggatctcggt
      301 cgtcccggcg gcgccagcgg gggccgcgct cggcccgtgc agagccgtca agaccggggt
      361 cgggaccttc ctcaggcccg gctgggagga agtcgccatc gcgggcgcat acacggcccc
      421 cgccggggcg atcggcgtcg agctgacctg cagcgtcgtc gcgaacgacc gcgtctacgc
      481 gagcgtctcg gacgaggtgc ccgggccggt cgcggtgctc gcaggtaccg ggtccgtccc
      541 cacgaacgcc gcgatcacga cgtgctacac gctgcgcgtg gcctacgtgg accgtagcta
      601 cacgacctac acctgtccgt agcgcgcgaa cgacgagatg ggccctcccg aatcgggagg
      661 gtcttttctt ttctagggga agagaccgcg cgtcaggtgc gcctcggcca cgcggccgac
      721 gccgatccag tgcgccgcct gacgcatcgt caggtccttc gcctgggcga gcgcgtagac
      781 ctcgtcgaac gacttctcca tgatctggcg gagacggtcg ttcacgtcct cttcgctcca
      841 gaagtacgcc tggatgtcct ggacccactc gaagtacgag accgtcacgc cgccggagtt
      901 cgccaggatg tcggggatca cgaagatgcc cttgtcgttc aggatcttgt cgccctcgaa
      961 cgtgacgggg ccgttggccg cctcgcacac gatcttggcc cgcacctggt ccgcgttctt
     1021 caccgtgatc acgccctcga tcgcggcggg cacgagcacg tcgcagtcga tctcgagcag
     1081 ctcgtcgttc gtgatggcgt cggccccggg gaagcccgcc accgtcccgg cctcctgctt
     1141 gtggcggttg atcgcctcgg ggtcgaggcc gcgctcgcgg tagagcccgc cgtcgacgtc
     1201 cgacacggcg acgacgcggc agcccgcgtc gtgcagcagc tgggccgcga acccgccgac
     1261 cttcccgtat ccctggatcg cgaccgtgac ctcctccggc gcgatgccca gggccttcag
     1321 cgtcgagaag atcatgaaca tcacgccgcg cgacgtcgcg ccgccgcggc ccttcgaacc
     1381 gccgatcgct accggcttgc ccgtgaccac gccggggacc gagtacccct tgttctggga
     1441 gtacgtgtcc atgatccacg acatgatctc ttcgttcgtg ttcatgtcgg gggccgggat
     1501 gtcgcgctcg gggccgatca gcgggaggat ctccgacgcg tagcggcgcg tcatgcgctc
     1561 gagctcgccg cggctcagcg tgcgcgggtc gaccgcgacg ccgcccttcg cgccgccgaa
     1621 cgggatgccg gcgatcgcgc acttccacgt catccacatc gcgagcgcct tgacctcgcc
     1681 gaggcctacg tccgggtgat agcggatccc gcccttggcg gggccgcgcg ttacgttgtg
     1741 gtggacgcgg tagccctggt agacgcgggt cgagccgtcg tccatgcgga acgggactga
     1801 cacgatcagc gcgcgcttcg ggttccggag agtgacgtgg acgcccggcg cgaggcccat
     1861 gagctccgcg gcctcgtcga gctgcgcgag cgcggtctcc caggcggtgt cgccgggagt
     1921 ctcgagcggg ctagtcgtcc cttccatgac cacgagctgg agacaggagt cgaacctgcg
     1981 acctgccgct tacaaggcgg ctgctctacc aactgagcta ctccatcgca acgctttgtc
     2041 gccgagggta ccccgcgggg agttcagact tgggcttcgg cgaggtggtg ctcgagcttg
     2101 cgcttgatcc tctggagcgc gttgtcgatc gacttcacgt ggcggccgag catgtcggcg
     2161 atctcct
//
LOCUS       DASQDO010000228         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_390481, whole genome shotgun sequence.
ACCESSION   DASQDO010000228 DASQDO010000000
VERSION     DASQDO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390481"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VEV43_06570"
     CDS             complement(<1..698)
                     /locus_tag="VEV43_06570"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HYP23218.1"
                     /translation="MRRACVVSLVAGLVWLLGGGAAQAKEFTLPAAEVHVKVRADGSV
                     RVTEHITYSFDGSFRGGFREIPLRPGEQLVDAQVSEGGEAYAPGAPASIGSSGSPGTF
                     GTADTGTGVRIVWHYDATDEERTFTIEYTLVGLAEAWDDVVDVYLQVWGDEWDVELER
                     LSGRVIVPGAQEDSVRVWGHAAGVDGATALLDGGRGASFVAEHVPPGQFVEVRVVFPR
                     SRLASTDGAKLRSG"
     gene            799..>1880
                     /locus_tag="VEV43_06575"
     CDS             799..>1880
                     /locus_tag="VEV43_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP23219.1"
                     /translation="MPKRVLIIGWDGVDWKILQPMLDSGELPNLKALIDRGAYGDCLS
                     TVPSHSWCAWPSFMTGLNPAGHAVFDILEHKPGATKRLPVTYKSIKNRTIFEDFTDAD
                     KTSLAINIPLTFPPPMIKGKVIGGGVLPASRSYTHPVELQQELDRNAPFPVNGMSWTT
                     YRNRPEAFLEECAEITAKRQKSFEYLMDTTDWDFGVLVYVSTDRIQHCLMEYIHPEHP
                     AYPEMKDTGVAKQCRAVYQQLDDGLAKLLERTNDDDLVIFMSDHGHQACTRAVTMDRV
                     LQHLGYLEFGRGSFAFNHIRWGPGRRIARRIYDILKLHGRISIPASPIEWSKTKAYTS
                     VVSTGEGVSVNLKGREPGGTVDPKDF"
BASE COUNT          322 a          692 c          602 g          264 t
ORIGIN      
        1 tgtccggacc ggagcttcgc cccgtccgtc gacgcaagcc ggctgcgcgg gaacacgacg
       61 cgcacctcga cgaactgccc gggcggcacg tgctccgcca cgaacgacgc gccgcggccc
      121 ccgtcgagca gtgcggtcgc accgtcgacc ccggcggcgt gcccccatac gcgcacgctg
      181 tcctcctgag cgcccggcac gatcacgcgg ccgctcaagc gctcgagctc gacgtcccac
      241 tcgtcgcccc acacctgcag gtagacgtcg acgacgtcgt cccacgcctc ggcgagcccg
      301 accagcgtgt actcgatcgt gaacgtgcgc tcctcgtcgg tggcgtcgta gtgccacacg
      361 atccgcacgc ccgtcccggt gtcagcggtg ccgaacgtcc ccggtgagcc cgagctcccg
      421 atcgatgccg gcgcgcccgg cgcgtacgcc tcgccgcctt ccgagacctg cgcgtcgacg
      481 agctgctcgc ccggccgcag cgggatctcg cggaagcccc cccggaacga gccgtcgaac
      541 gagtaggtga tgtgctcggt gacccggacg gacccgtcgg cacggacctt cacgtgcact
      601 tcggctgcgg gaagggtgaa ctccttcgcc tgcgcggccc cgccgccgag cagccacacg
      661 aggcccgcga cgagagagac gacgcaggct cgacgcattc ggctgctaac ctcccaggcc
      721 taggggtccc acaccctaat catccggacg ggccgcgggt tcggcccgcc ccacccgact
      781 ccacaggaag tgagacgagt gcccaagcga gttctgatca tcggatggga cggcgtcgac
      841 tggaagatcc tccagccgat gctcgactcc ggcgagctgc cgaacctgaa ggccctgatc
      901 gacagggggg cctacggcga ctgcctgtcg acggtgccgt cgcactcgtg gtgcgcgtgg
      961 ccgtcgttca tgaccggcct caaccccgcg ggccacgcgg tgttcgacat cctcgagcac
     1021 aagccggggg cgaccaagcg ccttcccgtc acgtacaagt ccatcaagaa ccggacgatc
     1081 ttcgaggact tcaccgacgc ggacaagacg tcgctcgcga tcaacatccc gctgacgttc
     1141 ccgcccccga tgatcaaggg gaaggtgatc ggcggcggcg tgttgccggc gagccgttcg
     1201 tacacgcatc ccgtcgagct gcagcaggag ctcgaccgca acgccccgtt cccggtcaac
     1261 gggatgagct ggacgaccta ccgcaaccgg cccgaggcgt tcctcgagga gtgcgccgag
     1321 atcacggcca agcggcagaa gtcgttcgag tacctgatgg acacgaccga ctgggacttc
     1381 ggcgtgctcg tctacgtctc gaccgaccgc atccagcact gcttgatgga gtacatccac
     1441 ccggagcacc ccgcgtaccc cgagatgaag gacacgggcg tcgcgaagca gtgccgcgcg
     1501 gtgtaccagc agctcgacga cggtctcgcg aagctgctcg agcggacgaa cgacgacgac
     1561 ctcgtgatct tcatgtcgga ccacggccac caggcgtgca cgcgcgcggt gacgatggac
     1621 cgcgtgctcc agcacctcgg ctacctcgag ttcggccgcg gctcgttcgc gttcaaccac
     1681 atccgctggg ggccgggccg ccgcatcgcg cgccggatct acgacatctt gaagctgcac
     1741 ggccgcatct cgatcccggc gtcgccgatc gagtggagca agaccaaggc ctacacgagc
     1801 gtcgtctcga ccggcgaggg ggtctcggtc aacctcaagg gccgagagcc cggtggcacc
     1861 gtcgacccga aggacttcga
//
LOCUS       DASQDO010000229         6880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_23066, whole genome shotgun sequence.
ACCESSION   DASQDO010000229 DASQDO010000000
VERSION     DASQDO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23066"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VEV43_06580"
     CDS             complement(<1..432)
                     /locus_tag="VEV43_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HYP23220.1"
                     /translation="MVHRPDRAEFARLLEDNVIVPVWREVLADALTPVGAFLRLDPRP
                     HGFLLESVEGGERWARYSFIGGDAFGVVTAKDGSVSLEGHAPVAPEQGEAPLAYVGRL
                     LEACRAPALEGLPPLHGGAVGFVGSDCVRELERLPDVPEDDL"
     gene            complement(440..751)
                     /gene="hisI"
                     /locus_tag="VEV43_06585"
                     /pseudo
     CDS             complement(440..751)
                     /gene="hisI"
                     /locus_tag="VEV43_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            complement(754..1896)
                     /locus_tag="VEV43_06590"
     CDS             complement(754..1896)
                     /locus_tag="VEV43_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYP23221.1"
                     /translation="MTTYLIYDDTLRSPELRHEIAEPLGDPVVFIEHEGKRIVVAGVF
                     EVPMLSRREDVVDEVWDFEELGRRDLISDKDFPEIHIGPEIVARAVKRVGADKVCVPA
                     TFWALAADYLRDQGIDVVVDPDEWARRRRRKTPWELEGIERAQRATETAMLTAARMLR
                     DAEPAPENRLRFEGEILTAEWIREVMSAELLAQGAESEEILVHSGDACLSGHDPGTGP
                     ILQDQTCIIDCFPRDRRSGAYTDMTRTFVPGRPSDDVVKLHGHVRKALEVAYEHVKPG
                     SAVAHAKVVEYFHSKGFPTLDHHQGPGHPERGFNHGLGHGVGLQVHERPWLSRRPDTL
                     AVGDVVAIEPGLYFEGIGGVRLEDTVLVTESGFEHFTDPFPYDLQP"
     gene            complement(1893..2672)
                     /gene="hisF"
                     /locus_tag="VEV43_06595"
     CDS             complement(1893..2672)
                     /gene="hisF"
                     /locus_tag="VEV43_06595"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HYP23222.1"
                     /translation="MLAKRVVPCLDVDHGRVVKGVRFRQLRDAGDPVELAARYDAEGA
                     DELVYLDITASSEGRNLILDIAARTAETLFIPLTIGGGVRSVDDVRKLLRAGADKVAI
                     NTAALDDPLLLRRAADEFGSQAVVVAIDARRDPKNKSWTVFAEGGRRDTGKDAVAWAE
                     QATTEYGAGEILLTSMDADGTTNGYDLDLTRAVASATPVPVIASGGAGQPGHLADALD
                     PAKGNADAALAASVFHFGTFSVDDAKRAIAERGLPVRIAAR"
     gene            2830..3438
                     /locus_tag="VEV43_06600"
     CDS             2830..3438
                     /locus_tag="VEV43_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23223.1"
                     /translation="MLVIAMPAAPAVASEDASDPPTVSGPLFGPEPSCHEDEFRGMRT
                     QECSWWLNLAPAETNLTEDYRAEWVQLEMTPPPGSCATNMVFGLGLPDSARLVSAAPA
                     QSARVRRAAPAVTRLDVDAEGAALVPGTVENEFVSAPGKVRVAADDGAYVYEWTGRWP
                     DKVVVALGLQMAGERIPPEVASTWFGVQGFEFGPCGSPRSGR"
     gene            complement(3512..4282)
                     /gene="hisA"
                     /locus_tag="VEV43_06605"
     CDS             complement(3512..4282)
                     /gene="hisA"
                     /locus_tag="VEV43_06605"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HYP23224.1"
                     /translation="MSFTVFPAVDISQGKCVRLLQGRFGTETVYSDDPVKVALGFCAA
                     GARWLHIVDLDGAKTGVRENRELVLEVVRRAACPVQAGGGMRTLDDVEEVIAAGANRA
                     VLGTVALEDPEAAAKACKTYGERIAVSLDARGGELATHGWTVGTGVPVLEAVKAFQDA
                     GVSLFVYTDVTRDGTMTGPDVEGLRRLTEATSLPVVASGGVSSLEDLRTVARLKPDGV
                     AGAVVGRALYEGKFGVGEANYAADEAAAGRSEPPLRER"
     gene            complement(4279..4830)
                     /gene="hisH"
                     /locus_tag="VEV43_06610"
     CDS             complement(4279..4830)
                     /gene="hisH"
                     /locus_tag="VEV43_06610"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYP23225.1"
                     /translation="MDHGMGNLRSVAKALEAAGAEVRLTASPSDAASADALCVPGQGI
                     FGRCMTALGSNGFDDLVREWTGAGRPFLGICLGMQVLFESSEEGGVPGLGLLPGEVRR
                     LPDRVTVPHIGWNTVGTTYYYFDHSFAVFPGDEAIVAGWAEHGDRFAATVRAGSILGV
                     QFHPEKSGRDGIALLREWVEEAA"
     gene            complement(4842..5426)
                     /gene="hisB"
                     /locus_tag="VEV43_06615"
     CDS             complement(4842..5426)
                     /gene="hisB"
                     /locus_tag="VEV43_06615"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HYP23226.1"
                     /translation="MTRSGSVKRETNETSISVELDLDGGEVSVSTGVPFIDHRLDQVG
                     RHARFGLHIEARGDLEVDAHHTVEDTGICIGEALAQALADKAGIARYGDATVPMEEAL
                     AQIALDLSGRPLLVYDAPMPAETIGQYESVLTEEFLQALCRSGGINMHVRLLAGRNAH
                     HATEAIFKCLARALASAVTVDPRGGIPSTKGTLA"
     gene            complement(5423..6478)
                     /gene="hisC"
                     /locus_tag="VEV43_06620"
     CDS             complement(5423..6478)
                     /gene="hisC"
                     /locus_tag="VEV43_06620"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015401261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYP23227.1"
                     /translation="MLPLRPDLEDVEPYVSPQLPARVRLNTNESPYAPPRTLTDALLR
                     EVEGMSLNRYPDRDARRLVDALAAKWDWPAEGIWVANGSNEVFMHLFLAYGGPGRTSL
                     TFEPTYSLHSLIPRLALTEVAQLPRDDSFAVDVAAASDWIEENRPAVAIVCSPNNPTG
                     NSTPLDVVAALCDAAPGLVVVDEAYGEFAPDGTSAIALLREHDNLVVVRTFSKAWRLA
                     GVRIGYLLGAPELVSAMAVARLPYHLSTLSQAVGIAALEAEDETLEAVGEIVAERDRI
                     AAGLADLGLRVMPSDASFVLFEVPDTAATWRALYERGVLVRSYERSAVLGSFLRVTVG
                     LSDENDDFLRTLEEVMA"
     gene            complement(6481..>6880)
                     /locus_tag="VEV43_06625"
     CDS             complement(6481..>6880)
                     /locus_tag="VEV43_06625"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYP23228.1"
                     /translation="EGGVAVLVRAVAHAMETVNAHAPEHLQLCFTGALDALDDVRNAG
                     SVFVGAYSPVAIGDYVGGTNHVLPSGGAARWASGLGAADFVKRIYVSALEPGALERLA
                     PHVEALAEAEGLSGHARSVRVRLDQRRGLG"
BASE COUNT          965 a         2451 c         2457 g         1007 t
ORIGIN      
        1 gaggtcgtcc tcgggcacgt ccggcagccg ctctagctcg cgtacgcagt cggagccgac
       61 gaagccgact gctccgccgt gcagaggcgg gaggccttcc aacgccggag cgcggcacgc
      121 ttcgagcagg cgaccgacgt acgcgagcgg tgcctctcct tgctccggcg cgaccggcgc
      181 gtggccttcc agcgagacgg atccgtcctt cgccgtcacc acgccgaacg cgtcgcctcc
      241 gatgaaggag tagcgggccc agcgctcgcc gccctcgacg ctctccaaca ggaagccgtg
      301 ggggcgcggg tcgagccgca ggaacgcgcc gaccggcgtc agcgcgtcgg ccagcacctc
      361 ccgccacacg gggacgatga cgttgtcttc gagcagccgc gcgaactccg cccggtcggg
      421 cctgtgcacc atcgcgtcag aagctccggt agaagcaggt gtcctcgccg gtgtggcacg
      481 cgggcccggc gggctcgacg agcatcagca cggcgtccgc gtcgcagtcg tagcgcacct
      541 cgacgacctt ctgcgtgttc ccggaggtgg cccccttgtt ccagtactcg cggcgcgacc
      601 tgctccagaa ccacgtcgtg ccggtgtcga gcgtgcggcg cacggcctcg gcgtcgaccc
      661 acgcgagcat cagcacgtcc ttgctggtgg cgtcctggac gataaccggc gcgaggcccc
      721 ggtcgtcgaa cacgacgtcc tcgggcctca ccgtcagggc tggaggtcgt aggggaacgg
      781 gtccgtgaag tgctcgaagc cggactcggt gacgaggacc gtgtcctcga gacggacgcc
      841 gccgatgccc tcgaagtaga ggccgggctc gatcgcgacg acgtcaccga cggcgagagt
      901 gtcgggccgg cggctcagcc acggccgttc gtggacctgg agcccgacgc cgtggccgag
      961 tccgtggttg aagccgcgct cggggtggcc ggggccctgg tggtggtcga gcgtcgggaa
     1021 ccccttcgag tggaagtact cgacgacctt cgcgtgcgcg acggccgagc ccggcttgac
     1081 gtgctcgtac gcgacctcga gggccttgcg gacgtggccg tgcagcttga cgacgtcgtc
     1141 cgaggggcgc ccgggcacga acgtgcgcgt catgtccgtg tacgcgccgg acctgcggtc
     1201 gcgcgggaag cagtcgatga tgcacgtctg gtcctggagg atcgggccgg tgccggggtc
     1261 gtgtccgctc aggcaggcgt cgcccgagtg cacgaggatc tcctcgctct cggccccctg
     1321 cgcgagcagc tccgcgctca tgacctcgcg gatccactcc gcggtgagga tctcgccctc
     1381 gaagcggagc cggttctccg gcgcaggctc ggcgtcgcgc agcatgcggg ccgcggtcag
     1441 catcgcggtc tcggtcgctc tctgagcgcg ctcgatccct tcgagctccc acggcgtctt
     1501 gcgacgccgg cggcgggccc actcgtccgg gtcgacgacg acgtcgatgc cctggtcgcg
     1561 cagatagtcg gccgcgaggg cccagaacgt ggccgggacg cacaccttgt cggcgccgac
     1621 gcgcttgacg gcgcgggcga cgatctcggg cccgatgtgg atctcgggga agtccttgtc
     1681 cgagatgagg tccctgcgcc cgagctcctc gaagtcccag acctcgtcga ccacgtcctc
     1741 gcgccgcgac agcatgggga cctcgaacac cccggctacg acgatgcgtt tgccctcgtg
     1801 ctcgatgaag acgacggggt cgccgagggg ctcggcgatc tcgtgccgga gctccgggct
     1861 ccgcagggtg tcgtcgtaga tcaggtaggt cgtcatcggg cggcgatcct aacggggagc
     1921 cctcgctcag ctatcgcccg cttggcgtcg tcgacggaga acgtgccgaa gtggaagacc
     1981 gaggcggcga gggccgcgtc ggcgttcccc ttggccgggt cgagcgcgtc ggcgaggtgg
     2041 cccggttggc cggcgccgcc ggatgcgatg acggggacgg gggtcgcgct cgccacggct
     2101 ctcgtgaggt cgaggtcgta gccgttcgtg gtgccgtcgg cgtccatcga ggtgaggagg
     2161 atctcgccgg ccccgtactc ggtcgtggcc tgctcggccc aggcgacggc gtccttgccg
     2221 gtgtcccggc ggccgccttc cgcgaagacc gtccagctct tgttctttgg gtcacgccgg
     2281 gcgtcgatcg cgacgacgac ggcctgggag ccgaactcgt ccgcagcccg gcggagcagc
     2341 agcggatcgt cgagggccgc ggtgttgatc gcgaccttgt cggccccggc gcgcaggagc
     2401 ttgcggacgt cgtcgaccga gcgcacgccg ccaccgatcg taagcgggat gaagagggtc
     2461 tcggcggtgc gggcggcgat gtcgaggatg aggttccggc cctcgctgga agcggtgatg
     2521 tcgaggtaga cgagctcgtc ggcgccctcg gcgtcgtagc gggccgccag ctcgacggga
     2581 tcgccggcgt cacggagctg gcggaaccgg acgcccttga cgacgcggcc atggtcgacg
     2641 tcgagacagg gaacgacccg cttggcgagc atcgcgggag cctacgggag cgacctgtga
     2701 ggaaaaacgg acgctatgcg gccatttctc ctcacaggtc cgtcggcgtc ggcccacggc
     2761 tccgggaagg tcccggggcg ctgcatggcg aatgactccc ccgtgcggac gcgttttctc
     2821 gttgtgctct tgctggtcat cgccatgccc gcggcccccg cggtcgcgag cgaggacgcc
     2881 tcggaccccc cgacggtatc ggggccgctg ttcgggccgg agccgagctg ccacgaagac
     2941 gagttccgag gcatgcgcac gcaggagtgc tcgtggtggt tgaacctcgc cccggccgag
     3001 accaacctca ccgaggacta ccgggctgag tgggtccaac tcgagatgac cccgccgccc
     3061 ggctcgtgcg ccaccaacat ggtcttcggc ctgggcctcc ccgacagcgc gcgcctcgtt
     3121 tcggccgcac cggcgcagag cgctcgcgtc cgccgcgccg cgcccgccgt cacccggctg
     3181 gacgtcgacg cggagggggc agcgctcgtg cccgggacgg tcgagaacga gttcgtgtcc
     3241 gcgcccggga aggtccgggt cgcggccgac gacggcgcct acgtgtacga gtggacgggc
     3301 aggtggccgg acaaggtcgt cgtcgccctg ggcctccaga tggcgggaga gcggatcccg
     3361 cccgaggtgg cgtcgacgtg gttcggcgtc caggggttcg agttcggccc gtgcgggagc
     3421 ccgcgcagcg gccgctgagc cctccgcccg ccgcgtgcgc accaggttgc gcattccgca
     3481 actgactgcg cacgcgaggg ggccgcgtcg gtcagcgttc gcggagcggg ggctcggagc
     3541 ggcctgccgc ggcttcgtcg gctgcgtagt tcgcttcgcc caccccgaac ttgccctcgt
     3601 atagggcccg gcccactact gcgccggcta ctccgtccgg cttcaggcgg gccaccgtgc
     3661 gcaggtcttc gagggaagac acgccgcccg aggcgaccac cggcagtgac gtggcttcgg
     3721 tcaggcggcg gaggccctct acgtccgggc ccgtcatcgt cccgtccctc gtcacgtccg
     3781 tgtagacgaa cagcgacacg ccggcgtcct ggaaggcctt caccgcttcg agcaccggca
     3841 cgccggttcc gacggtccag ccgtgcgtcg caagctcgcc cccgcgggcg tcgagcgaca
     3901 ccgctatgcg ctcgccgtac gtcttgcacg ccttggcggc ggcctcggga tcctccagcg
     3961 ccaccgtccc gagcaccgca cggttcgcgc cggccgcgat cacctcctcg acgtcgtcga
     4021 gcgtgcgcat gccccctccg gcctggaccg ggcaggccgc ccggcggacg acctcgagca
     4081 cgagctcccg gttttcgcgc acgcccgtct tggctccgtc gaggtcgacg atgtgcagcc
     4141 acctcgcccc cgccgcgcag aagcccagcg cgaccttgac cgggtcgtcg gagtagacgg
     4201 tctcggtccc gaaccgcccc tgcaggagcc ggacacactt gccctgcgat atgtcgaccg
     4261 cggggaacac cgtgaagctc acgccgcctc ctctacccac tcgcgcagca acgcgatccc
     4321 gtcgcggccg gacttctccg ggtggaactg gacgccgagg atcgatccgg cccggaccgt
     4381 cgccgcgaag cggtcgccgt gctcggccca tccggcgacg atcgcctcgt cgccggggaa
     4441 cactgcaaag gagtggtcga agtagtaata ggtggtcccc accgtgttcc agccgatgtg
     4501 cgggaccgtg acccggtccg ggagcctcct cacctcgccg gggaggaggc cgaggccggg
     4561 gacgccgccc tcctcgctcg actcgaacag cacctgcatg ccgaggcaga tcccgaggaa
     4621 cggtcgtccg gcccccgtcc actcccgcac gaggtcgtcg aacccgttcg agccgagcgc
     4681 ggtcatgcag cggccgaaga ttccctgccc gggcacgcac aaggcgtcgg cagaagcagc
     4741 gtcggacggg gacgcggtga gccgtacctc cgcgcccgca gcttccagcg ccttggcgac
     4801 gctgcggagg ttccccatcc cgtggtccac cagggcgacg ttcaggcgag cgtccccttg
     4861 gtcgacggga tgccgccgcg cgggtcgacc gtgacggcgg aggcgagggc ccgggccagg
     4921 cacttgaaga tcgcctcggt cgcgtggtgc gcgttgcgtc ccgcgagcaa acggacgtgc
     4981 atgttgatcc cgccgctgcg gcacagggcc tggaggaact cctcggtgag aaccgactcg
     5041 tactgcccga tcgtctccgc gggcatcggc gcgtcgtaga cgagcaacgg cctcccggag
     5101 aggtcgagcg cgatctgcgc gagcgcctcc tccatcggca ccgtggcgtc gccgtagcgc
     5161 gcgatcccgg ccttgtcggc gagggcctga gcgagggcct cgccgatgca gatgccggtg
     5221 tcctcgacgg tgtgatgcgc gtccacctct aggtccccgc gtgcctcgat gtgcagacca
     5281 aagcgtgcgt gacgtccgac ctggtcgagc ctgtggtcga tgaacgggac gcccgtcgag
     5341 acggagacct cgccgccgtc gaggtcgagc tcgactgaga tcgacgtctc gttcgtctcg
     5401 cgcttcacgc tccccgacct cgtcacgcca tgacctcctc gagggtacgg aggaagtcgt
     5461 cgttctcgtc cgacagcccg accgtgacgc gcaggaacga gccgagcacc gccgaccgtt
     5521 cgtacgaccg cacgagcacg cctcgctcgt acaaggccct ccaggtggcg gccgtgtccg
     5581 ggacctcgaa cagcacgaag ctcgcgtccg acggcatcac gcgcaggccg aggtcggcca
     5641 ggcccgctgc gatgcgatcc cgctccgcca cgatctcgcc cacggcctcc agcgtctcgt
     5701 cctcggcttc gagcgccgcg atccccaccg cctgcgacag cgtcgacagg tggtacggga
     5761 gccgcgctac cgccatcgcc gacacgagct ccggcgctcc gaggaggtag ccgatccgca
     5821 cccccgcgag gcgccacgcc ttcgagaacg tccggacgac gacgaggttg tcgtgctcgc
     5881 gcagcagcgc gatggcgctc gtgccgtccg gcgcgaactc gccgtaggcc tcgtcgacga
     5941 cgaccagtcc cggcgcggca tcgcagagcg cggcgacgac gtccaggggg gtcgagttgc
     6001 ccgtcgggtt gttcggcgag cagacgatcg cgaccgccgg ccggttctcc tcgatccaat
     6061 ccgaagcggc agcaacgtcg actgcgaacg agtcgtcgcg cggaagctgc gccacctccg
     6121 tcagcgcgag gcgcgggatc agcgagtgga ggctgtacgt cggctcgaac gtcagcgacg
     6181 tccggcccgg ccccccgtac gcgaggaaca gatgcatgaa gacctcgttc gagccattcg
     6241 cgacccagat cccttcggcc ggccagtccc acttcgcggc cagcgcgtcg accagccggc
     6301 gcgcgtcgcg gtcggggtag cggttgaggc tcatgccctc gacctcgcgc aacagcgcgt
     6361 cggtcagcgt gcggggtggc gcgtacggcg actcgttcgt gttgagacgg acgcgcgcgg
     6421 ggagctgcgg ggagacgtag ggctcgacgt cttcgaggtc cggtctgaga gggagcacgt
     6481 ctagccgagg ccccggcgct ggtcgaggcg gacccggacg gacctcgcgt gaccgctgag
     6541 accttctgcc tccgcgagcg cctcgacgtg cggtgcgagg cgttcgagcg cacccggttc
     6601 gagcgccgac acgtagatgc gcttgacgaa gtcggcggcg cccaggcccg acgcccagcg
     6661 cgccgcgccg ccactcggaa ggacgtggtt cgtcccgccg acgtagtcgc cgatcgcgac
     6721 cggcgagtag gcgccgacga agaccgagcc cgcgttgcgc acgtcgtcga gggcgtcgag
     6781 cgctcccgtg aagcacagct ggaggtgctc gggtgcgtgt gcgttgacgg tctccatcgc
     6841 atgcgcgacg gcgcgcacga gcaccgcgac gccgccctcg
//
LOCUS       DASQDO010000230         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_879953, whole genome shotgun sequence.
ACCESSION   DASQDO010000230 DASQDO010000000
VERSION     DASQDO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879953"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VEV43_06630"
     CDS             <1..477
                     /locus_tag="VEV43_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HYP23229.1"
                     /translation="AVEAVSGARGDSTPAGAVATATPEPTDKPEPTPEPETAKPEEPS
                     ETPKPESTPKPTPEPKEETEPLITEGITVQVLNATDVADADDRMADKLSKLGFTVVAV
                     ESASSRYEATTVFWSTEEAKEAAQRLADRFGWTAQVKPENLSTTVSLHVVVGAVEG"
     gene            complement(474..1262)
                     /locus_tag="VEV43_06635"
     CDS             complement(474..1262)
                     /locus_tag="VEV43_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23230.1"
                     /translation="MPRRLRALPLLLLSLAACRPDTVDLAYRFPEDERQYRLEAEISS
                     EWDVGQPGGGSCRVVLDVSENVREQDGDTAVVEVEMSPVDVQEDGLGCPRGGGFALRI
                     DTNGRVLDVLEVDGVDAADLAQEEEAFIGTYRPTLPGEPVGLGDTWESELEPEVGSLA
                     QLATEGALESLYMDDGGPVAELSYSGSGPLEWQTQLPQGTAGLGGTATTRATAKFDVD
                     RGVLVAASSTLRGDFDVRVLPPAGGGTPVTGTLSLDLELTVSAR"
     gene            1298..2074
                     /gene="otsB"
                     /locus_tag="VEV43_06640"
     CDS             1298..2074
                     /gene="otsB"
                     /locus_tag="VEV43_06640"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HYP23231.1"
                     /translation="MSDDLLRPFRERPERAGIFLDFDGTLSEIVHVPSDARPAEGAAD
                     VLRALAAAYAVVAIVSGRSAHQLVEWLGPEIEIWGVHGAQRATAGDVRLSPLAAPFAE
                     LMGEVHEAAAKALADVDLEGVILEDKEVMLGLHFRNARDVERARVVLDEIADELAARY
                     GLTRAGGRLAYELRPPAEFTKAAVVRERAAGLEAVAFAGDDRVDLPGFDALDELAGAG
                     AATLRIAVDSDEAPPELLERADVVVDGPPGATELLGKLLP"
     gene            complement(2071..>2306)
                     /locus_tag="VEV43_06645"
     CDS             complement(2071..>2306)
                     /locus_tag="VEV43_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23232.1"
                     /translation="GDHSVAIEDQMDVESTAAALERAIDMPRAERRERAAALRELSTA
                     RKPEDWINAQLDDLEAVQAGREPLSPPAPAPGR"
BASE COUNT          326 a          837 c          845 g          298 t
ORIGIN      
        1 gcggtcgagg ccgtcagcgg ggcgaggggc gactcgacgc ccgcgggggc cgtggcgacg
       61 gcgacgccgg agcccaccga caagcccgag ccgacgcccg agcccgagac cgcgaagccc
      121 gaagagccct ccgagacccc caagcccgag tcgacgccga agccgacgcc cgagccgaag
      181 gaagagaccg agccgctgat caccgagggc atcacggtcc aggtcctgaa cgcgaccgac
      241 gtggccgacg ccgacgaccg gatggcggac aagctctcga agctgggctt caccgtcgtc
      301 gcggtcgagt ccgcgtcgtc ccgttacgag gcgacgaccg ttttctggtc gaccgaggag
      361 gcaaaggagg cggcgcagcg cctggccgac cggttcggct ggacggcaca ggtcaagccc
      421 gagaacctct cgacgacggt cagtctgcac gtcgtcgtcg gcgccgtcga gggctagcgc
      481 gccgagaccg tcagctccag gtccaggctc agcgtcccgg tgaccggcgt ccctccgccc
      541 gccggaggca ggacccgcac gtcgaagtcc ccacgcaggg ttgacgacgc ggccaccagg
      601 accccgcggt cgacgtcgaa cttcgccgtg gcccgggtcg tggccgtgcc gccgagccct
      661 gcggtcccct gcggcagctg cgtctgccat tcgagggggc cggagcccga gtacgacagc
      721 tccgcaacgg ggcccccgtc gtccatgtag agcgattcga gcgcgccctc cgtcgcgagc
      781 tgcgcgagcg agcccacctc cggctccagc tccgactccc aggtgtcgcc gagaccgacc
      841 ggctcgcccg gaagggtcgg ccggtaggtc ccgatgaagg cctcctcttc ctgcgcgagg
      901 tcggccgcgt cgacgccgtc cacctcgagg acgtcgagca cgcggccgtt cgtgtcgatc
      961 cggagcgcga agccaccccc ccgcgggcag ccgagcccgt cttcctggac gtcgacgggc
     1021 gacatctcga cctcgacgac cgcggtgtcg ccgtcctgct ctcgcacgtt ctccgacacg
     1081 tcgagcacga cgcggcacga gcctccgccg ggctggccga cgtcccactc gctcgagatc
     1141 tcggcctcga ggcggtactg ccgctcgtcc tccgggaagc ggtacgcgag gtcgacggtg
     1201 tcgggccggc aggccgcgag ggaaaggagc agcaacggca gcgcgcggag ccggcggggc
     1261 atgggcggca gtctagtggc cggtacgctg caaagcggtg tcagacgacc tcttgcgtcc
     1321 cttccgcgag cgaccggaac gggccggcat cttcctcgac ttcgacggca cgttgtccga
     1381 gatagtccac gtgccctccg acgcgcgccc ggcggaaggt gccgccgacg tcctgcgggc
     1441 gctggccgcg gcctatgcgg tcgtcgcgat cgtgtcggga cgctcggccc accagctcgt
     1501 cgagtggctg ggcccggaga tcgagatctg gggcgtccac ggggcacagc gggcgacggc
     1561 cggcgacgtg cggctgtcac cgctcgccgc tccgttcgcc gagctgatgg gggaggtgca
     1621 cgaagccgcg gcgaaggcgc tggccgacgt cgaccttgaa ggggtcatcc tcgaggacaa
     1681 ggaagtcatg ctcggcctcc acttccgcaa cgcccgtgac gtcgagcgcg cccgcgtggt
     1741 cctcgacgag atcgcggacg agctggcggc tcggtacggc ttgacccgcg cgggagggcg
     1801 gctcgcttac gagctccgtc cgccggcgga gttcacgaag gccgcggtcg tgcgcgaacg
     1861 cgcggccggg ctggaggccg tcgcgttcgc aggagacgac cgcgtcgacc ttcccggctt
     1921 cgacgcgctg gacgagctcg ccggggcggg ggccgcgacg ctgcggatag ccgtggactc
     1981 ggacgaggcc cccccggagc tgctggagcg agcggacgtg gtcgtcgacg gcccgcccgg
     2041 ggcgaccgag ctcctcggaa agctcctgcc ctagcggccg ggggccgggg ccggcggcga
     2101 cagcggctcg cggcctgcct gcacggcttc caggtcgtcg agctgcgcgt tgatccagtc
     2161 ctcgggcttg cgtgcggtcg agagctcgcg caaggccgcg gcgcgttcgc gccgctcggc
     2221 acgcggcatg tcgatggcac gctcgagcgc ggcggccgtc gactccacgt ccatctggtc
     2281 ctcgatcgcc accgaatggt cgccga
//
LOCUS       DASQDO010000231         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1124914, whole genome shotgun sequence.
ACCESSION   DASQDO010000231 DASQDO010000000
VERSION     DASQDO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124914"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEV43_06650"
     CDS             <1..800
                     /locus_tag="VEV43_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYP23233.1"
                     /translation="GVAQGSAQPPRFIQLHYRPDNATAKVALVGKGITYDSGGLSLKD
                     AKSMETMKTDMSGAAAVIGAMGAVGRIRPNVEVLGLVPATENMPGGGAIKPGDVIRHY
                     GGTTVEVLNTDAEGRLVLADALAFASEQEPDAIVDVATLTGAIMVALGTRAAGCFSND
                     DDLAQRLIDAGAQEGERLWRMPLFADYAGDIESDVADLKNISGARWGGAIVAAVFLQQ
                     FVGKGIKWAHLDIAGAARAEGPSAEISKGGTGTAARTLVSWIEGFAR"
     gene            776..1087
                     /locus_tag="VEV43_06655"
     CDS             776..1087
                     /locus_tag="VEV43_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23234.1"
                     /translation="MDRRVRALSDDQVVPLGVEPPPDPPPARPSGLHGLAVTGMSLSA
                     LVGIVVLFLSIGIGLPSGPRRAAIAVVVGAAVVFLACASTAVFTAARETYPSRGRGDA
                     E"
     gene            1097..1297
                     /locus_tag="VEV43_06660"
     CDS             1097..1297
                     /locus_tag="VEV43_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23235.1"
                     /translation="MEIEGHPTQDLIDELERRGAVRVAGSSGGPRVEALRFLTERLGE
                     SPGYWMFLPYETFDTGFDEIPR"
     gene            1299..1775
                     /locus_tag="VEV43_06665"
     CDS             1299..1775
                     /locus_tag="VEV43_06665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYP23236.1"
                     /translation="MPVSPDAFRSALRRFASGVTIVTVASGDELHGMTASSFASVSLV
                     PPLVLVCLDKSSRTLELVSETKTFAVNVLRGDQQQVSVAFARPGLKPFESVSHRRGAN
                     GAPVLDDAIAVLECATYRVFEAGDHEVVLGEVTAVSAPGGDPLVYYDGAYRSLSES"
     gene            complement(1751..2095)
                     /locus_tag="VEV43_06670"
     CDS             complement(1751..2095)
                     /locus_tag="VEV43_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HYP23237.1"
                     /translation="MPVGLVVPRTGLVIAESVRWARSPRQRAKGLLATELGPGEALVI
                     EPARQVHTFGMAYPIDVCFCDAGWNVLHVVTPLRPRRVTRWVFRARYAVEAPAGVLEG
                     VRPGDQLSLSER"
     gene            2167..2328
                     /locus_tag="VEV43_06675"
     CDS             2167..2328
                     /locus_tag="VEV43_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HYP23238.1"
                     /translation="MQKWEYATVPLISHALQEILNQWGEEGWELVQVLESNATGTTAY
                     LKRPKNGAA"
     gene            complement(2383..3264)
                     /locus_tag="VEV43_06680"
     CDS             complement(2383..3264)
                     /locus_tag="VEV43_06680"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HYP23239.1"
                     /translation="MRVRKAVIPAAGLGTRLLPASKSLPKEMIPLVDRPGIQYVVEEC
                     ARAGIRDVLVITSRGKATMEDHFDRSLELEERLEKSGKRDELEEVRGVADLANVHYVR
                     QKEALGLGHAVAAARSFVGDEAFAVLLPDEIVPAPLGDEPPLLERMIDVHERDGVSVI
                     AVRRVPPDRVSAYGIVAPEGEMKDDFVPISDVIEKPAAADAPSDLGAVGRYLLAPEVF
                     DALDAAAPSVGGEIQLADGISALMGSPGVYAYLHRGPIYDVGKKLDYVKGTIQLALRR
                     DDVGPALKDWLRELLAE"
     gene            3323..4000
                     /locus_tag="VEV43_06685"
     CDS             3323..4000
                     /locus_tag="VEV43_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP23240.1"
                     /translation="MPQTILVIEDEETIAAAVAARLEKEGFDVVVAPDGLAGIERFRK
                     ETPDLVVLDLMLPGMDGLEVCRRIQAERYVPVLMLTARNDETDILVGLGVGADDYVTK
                     PFSPRELVARVKTLLRRVERSAAAPPATLRAGDIEIDTGRRLVHKAGALLHLTPIEFE
                     LLHRFAGKPGFVFTRDQLLNDVWGYDDPAGGRTVDTHVASLRRKLGSEVIRTVHGVGY
                     ALEVSTP"
     gene            3997..>4316
                     /locus_tag="VEV43_06690"
     CDS             3997..>4316
                     /locus_tag="VEV43_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HYP23241.1"
                     /translation="MRPLDRFSSIKTKLGIVIVAAVFVTVVTIIVGVRAGVPLRVCGV
                     IAAFLSLGMVQLLARGMTSPLREMAAAATAMSRGDYDRRVVATSRDEVGDLARAFNTM
                     AEEL"
BASE COUNT          631 a         1533 c         1513 g          639 t
ORIGIN      
        1 tcggcgtcgc gcagggctcg gcgcagcctc cgcgcttcat ccagctccac taccgccccg
       61 acaacgcgac cgcgaaggtc gcgctcgtcg gcaaggggat cacgtacgac tccggcgggc
      121 tctcgctgaa ggacgcgaag agcatggaga cgatgaagac cgacatgtcg ggggccgccg
      181 ccgtgatcgg tgcgatgggc gcggtcggcc gcatccgtcc gaacgtcgag gtgctcgggc
      241 tcgtgccggc gaccgagaac atgcccggcg gcggagcgat caagccgggc gacgtcatcc
      301 gccactacgg gggcacgacc gtcgaggtgc tcaacaccga tgcggagggg cggctcgtgc
      361 tcgccgacgc actcgcgttc gcgagcgagc aagaacccga cgcgatcgtc gacgtcgcta
      421 ccttgacggg ggcgatcatg gtcgcgctcg ggacgcgcgc cgcgggctgt ttctcgaacg
      481 acgacgacct cgcgcaacgg ctgatcgacg ccggcgccca ggagggcgaa cggctgtggc
      541 gcatgccgtt gttcgccgac tatgccggcg acatcgagtc cgacgtagcc gacctcaaga
      601 acatcagcgg cgcgcgctgg ggcggcgcga tcgtcgccgc ggtgttcctc cagcagttcg
      661 tcggcaaagg gatcaagtgg gcgcacctcg acatcgccgg tgccgcccgc gccgaggggc
      721 cgtcggccga gatctcgaag ggcggcacgg ggaccgcggc ccggacgctc gtgtcgtgga
      781 tcgaagggtt cgcgcgctga gcgacgacca ggtggtcccg ctcggggtcg agcccccgcc
      841 cgacccaccg cccgcgcgcc cttcgggtct ccacggcctc gcggtgaccg gcatgtcgct
      901 gtccgcgctc gtcgggatcg tcgtgctgtt cctgtcgatc gggatcgggc tcccgtccgg
      961 cccccgccgc gccgcgatcg cagtcgtcgt gggggccgct gtcgtgttcc tcgcatgcgc
     1021 gtcgaccgcc gtgttcaccg ccgcgcgcga gacgtatccg tcgagaggcc gcggggacgc
     1081 cgaatagact cggcgcgtgg agatcgaggg gcacccgacc caggacctga tcgacgagct
     1141 cgagcggcgg ggcgcggtgc gggtcgcggg ttcgtcgggg gggccgcgcg tcgaggccct
     1201 gcgcttcctc accgaacgcc tgggcgagag ccccggctac tggatgttcc tcccgtacga
     1261 gaccttcgac accgggttcg acgagatacc ccgctgacat gccggtctcg cccgacgcgt
     1321 tccgaagcgc gctgaggagg ttcgcgtccg gcgtcacgat cgtcaccgtc gcatccggcg
     1381 acgagctcca cgggatgacc gcgtcgtcgt tcgcgtcggt ctcgctcgtt cccccgctgg
     1441 tgctcgtatg cctcgacaag tcgtcgcgca cgctggagct cgtgtccgag acgaagacgt
     1501 tcgccgtcaa cgtcctgcgc ggcgaccagc agcaggtatc ggttgcgttc gcgcgtccgg
     1561 gcctcaagcc gttcgagtcg gtgtcgcacc gcaggggcgc aaacggggcc cccgtgctcg
     1621 acgatgcgat cgcggtcctc gagtgcgcga cctaccgcgt gttcgaggcc ggcgaccacg
     1681 aggtggtgct cggagaggtc accgcggtct cggcgcccgg cggcgacccg ctcgtctact
     1741 acgacggcgc ctaccgctcg ctgagcgaga gctgatcgcc gggccgcacc ccttcgagga
     1801 cgcccgccgg cgcctcgacc gcgtagcgcg cccggaagac ccaccgtgtg acgcgccgcg
     1861 gcctcagcgg cgtcacgacg tggaggacgt tccatcccgc gtcgcagaag cagacgtcga
     1921 tcgggtaggc catcccgaac gtgtggacct ggcgggcggg ctcgatcacc agcgcctcac
     1981 cgggccccag ctcggtggcg agaagaccct tcgctcgctg ccgcggagac cgcgcccagc
     2041 gcacgctctc ggcgatgacg agcccggtcc tgggtacaac gaggccgacc ggcactgaag
     2101 taggttaagc cgccgcctcg aagcgggctg cggcgcacac cacacctata tcggaggaga
     2161 cggcgaatgc agaagtggga gtacgcgacg gtgcccctca tctctcatgc gctccaggag
     2221 atcctcaacc agtggggcga ggagggctgg gagctcgtcc aggtgctcga gagcaacgca
     2281 acggggacga cggcctacct caagcggccg aagaacggcg cggcctagct tcgaaccgcg
     2341 ccggcccccc ggcgtattcc taacgaccgg ctcggcgaac cgctactcgg ccagcagctc
     2401 gcgcagccag tccttcagcg ccgggcccac gtcgtcgcgg cgtagcgcta gctggatcgt
     2461 ccccttgacg tagtcgagct tcttgccgac gtcgtagatc ggcccccggt ggaggtacgc
     2521 gtacacgccg ggcgagccca tcagcgcact gatcccgtcg gcgagctgga tctcgccgcc
     2581 gacgctcgga gcggccgcgt cgagcgcgtc gaacacctcg ggcgcgagca ggtagcgacc
     2641 gaccgcgccg aggtccgagg gcgcgtcggc cgcggcgggc ttctcgatca cgtcggagat
     2701 cggcacgaag tcgtccttca tctcgccctc gggggccacg atcccgtagg ccgagacacg
     2761 gtcgggcggc acccgccgca ccgcgatcac gctgacgccg tcgcgctcgt ggacgtcgat
     2821 catccgctcg aggagtgggg gctcgtcgcc cagcggcgcc gggacgatct cgtcggggag
     2881 cagcactgcg aacgcctcgt cgccgacgaa tgaccgcgcg gccgcgaccg cgtgcccgag
     2941 cccgagggcc tccttctgcc ggacgtagtg gacgttcgcg aggtccgcga ccccgcgcac
     3001 ctcttcgagc tcgtcacgct tgcccgactt ctcgagccgt tcctcgagct cgagcgagcg
     3061 gtcgaagtgg tcctccatcg tggccttgcc ccggctggtg atcacgagca cgtcgcgtat
     3121 cccggcgcgg gcgcattcct cgacgacgta ctggatgccc ggccggtcga cgagcgggat
     3181 catctccttc ggcagcgact tgctcgccgg aaggagcctc gtcccgagcc cggcggcggg
     3241 gatgactgcc tttcgaaccc tcacacgatc tccatatcgt ctcggtcact cccgaacaac
     3301 atcttcctac actggcccca ccgtgcccca gaccatcctc gtcatagaag acgaagagac
     3361 gatcgccgcc gccgtcgcag cccgcctcga gaaggagggt ttcgacgtcg tcgtcgcgcc
     3421 cgacgggctc gccgggatcg agcgcttccg caaggagacc ccggacctcg tcgtcctcga
     3481 cctgatgctg cccggcatgg acgggctcga ggtgtgccgg cgcatccagg ccgagcggta
     3541 cgtgccggtg ctgatgctga cggcgcgcaa cgacgagacc gacatcctcg tggggctcgg
     3601 tgtcggtgcg gacgactacg tgacgaagcc gttctcgccg cgcgagctcg tagcgcgggt
     3661 gaagacgctg ctgcggcgcg tcgagcgctc tgctgcggca ccgcctgcga ccctgcgcgc
     3721 cggtgacatc gagatcgaca ccggccggcg gctcgtccac aaggccgggg cgctgctgca
     3781 cctgacgccg atcgagttcg agctgctcca ccgcttcgcc ggcaagccgg ggttcgtgtt
     3841 cacgcgcgac cagctcctca acgacgtgtg ggggtacgac gacccggccg ggggccggac
     3901 ggtggacacg cacgtcgcgt ccctgcggcg caagctcggc agcgaggtca tccgcaccgt
     3961 ccacggcgtc ggctacgcgc tcgaggtctc aacgccgtga ggccgctcga ccggttctcg
     4021 tcgatcaaga cgaagcttgg catcgtgatc gtggcggccg tattcgtgac cgtcgtcacg
     4081 atcatcgtgg gagtgcgggc cggggtcccg ctcagggtgt gcggcgtgat cgccgcgttc
     4141 ctgtccctgg gcatggtcca gctgctcgcc cgcgggatga cctcgccgct gcgggagatg
     4201 gccgcagccg ccaccgcgat gtcgcgcggc gactacgaca ggcgcgtcgt ggcgacgtcc
     4261 cgcgacgagg tcggcgacct cgcgcgggcg ttcaacacga tggccgagga gctgag
//
LOCUS       DASQDO010000232         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_23164, whole genome shotgun sequence.
ACCESSION   DASQDO010000232 DASQDO010000000
VERSION     DASQDO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23164"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VEV43_06695"
     CDS             complement(<1..499)
                     /locus_tag="VEV43_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23242.1"
                     /translation="MRKAARTALAIVVLLGGLALLPARGFEAIAQPLSQPTPPLPPIL
                     PGGEPEPSPSPTESEEPGGGGGGNEPGGGGGRPGGGGEEDPKGEGGDGKDEPGDGKKK
                     DRKPGEDDRKGLDDKKKRKNKKKKDDDEVLPTPSIPGSFNTEELLATASRLRSLGLSE
                     HELASK"
     gene            complement(708..3026)
                     /gene="ftsH"
                     /locus_tag="VEV43_06700"
     CDS             complement(708..3026)
                     /gene="ftsH"
                     /locus_tag="VEV43_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYP23243.1"
                     /translation="MSSEAQGGFAAWRRKFAREFKQYRVRRRRKRQAGKKASGRRSTI
                     VLGLVLILLFGAFAWAQTYLEPQSPGTELSFDELDALAKENRIATAEFRDEDAQLVGT
                     FQAKPLEGYEDEPKKTEAAKGEDAKGENAQGDDAAGDDANAGGTGPANQGENAEGDAQ
                     GEGAAGGAGKDATKKPPAKPEPPSKAPEGEGTFWVQLPQNGAAFGPLVDSLTAAGASV
                     SVDPQTAKAIVRVVSQFLLPLLILAAFFGLLFTAGRGGGSGIGDVMQFGSIGKGKSKK
                     GASGMVTYKDVGGAEEAVLELSEVVDYLKNPDRYEDIGAVPPKGVLLFGPPGCGKTLI
                     ARATAGEAGVPFFSVAGAEFVESLVGVGAARVRDLFARVRAVAPAIVFIDELDAAGRK
                     RGAGGSGGGGSDEREQTLNQMLVEMDGFDVAAGIVVIGATNRPDILDPALLRPGRFDR
                     HITVDQPDHDGRKEILMLHAQGKPLAPDVDMDYIAKRTPGFSGADLANVINEAALLSL
                     RNAKSAIETTDLEEAIQRVLHGPKRRGKVLSDEERGRASYHEAAHALVSAALGRGDDV
                     HRVTILARSKNVAATGIETEDDSALLTRYQLTRQLVTSMAGLAAEEMIFGEPSTGSEN
                     DLERATEIARDMVARFGMSPRLGRARLLASDAEVYLGGDTGMSQLSDPTHTALDAEIG
                     RILAAAEHEAARLIEANLDVLHRMAEQLKELETLEGVPLHRILKEVKEGKLEMNELFS
                     TASTNGSKNGGAARRPRKAPAKTTSPGDTTEA"
     gene            complement(3063..>3843)
                     /locus_tag="VEV43_06705"
     CDS             complement(3063..>3843)
                     /locus_tag="VEV43_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23244.1"
                     /translation="EGGVDCDDDGDVEIPHGDPVVCKISPLRARQASLMAPAIIGMVE
                     SQIDAPRVCWEAPADSPVVEEGPTGCVEADGENPLGFDATFSCPQLESRKRFTLELGA
                     NVDGQKDDVSTVSVLCKVPPKPDPAKEPPPLPFIALPVAFIPPPPVPPEIPPVPNPNP
                     NPQPNPQSQAQAQAQAAMAHQEQQQPQLAYVYAQHQHAAAAAQESAGGKEAEYAMSSY
                     SKDSDGVPPEAIFMIGAMTMTAAFGCVSLAREKVRVQHVRR"
BASE COUNT          474 a         1347 c         1348 g          674 t
ORIGIN      
        1 ccttgctcgc gagctcgtgc tccgagaggc cgaggctgcg cagcctcgag gccgtcgcga
       61 ggagctcctc ggtgttgaag gagccgggga tcgacggggt cgggaggacc tcgtcgtcgt
      121 ccttcttctt cttgttcttg cgcttcttct tgtcgtcgag acccttgcgg tcgtcctcgc
      181 ccggcttgcg gtccttcttc ttcccgtcgc cgggctcgtc cttgccgtcc ccgccctcgc
      241 ccttcgggtc ctcttcgccg ccgccaccgg gacggccgcc gccaccgccg ggctcgttgc
      301 cgccaccgcc accgccgggc tcctcggact ccgtggggct cggggacggc tcgggctcgc
      361 cgcccggcag gatcgggggc agcggagggg tcggctgcga caggggctgt gcgatcgcct
      421 cgaacccgcg ggcgggcagc agcgctagtc cgcccaggag gacgacgatg gcgagagctg
      481 tgcgagctgc ttttctcact cgggtacggg tcctttggct tcgaagcgtg gtggaacgat
      541 cactgtaagg gacttggatt cgtagttctc cggcccccgc acctctcctg ttccaaccgg
      601 tcgcctgggc gagcggttat taggaagaac ggcaggcacg ggcgccgggt tgaccttatt
      661 tgtcccgctt ctccgggcca atggcccgtt tgggtggttc ctggacgcta ggcctcggtc
      721 gtgtcgcccg gggaggtcgt cttcgccggc gccttgcgcg gccgccgggc ggcccctccg
      781 ttcttggacc cgttcgtcga ggccgtcgag aacagctcgt tcatctcgag cttgccctcc
      841 ttgacctcct tcagtatccg gtggagcggg acgccttcga gcgtctcgag ctccttcagc
      901 tgctcggcca tcctgtggag cacgtcgagg ttggcttcga tgagccttgc cgcctcgtgc
      961 tccgccgcgg cgaggatccg gccgatctcc gcgtccaacg cggtgtgcgt cgggtcggag
     1021 agctgcgaca tcccggtgtc gccgccgagg tagacctccg cgtcggacgc cagcaggcgc
     1081 gcccggccga gccgcgggct catcccgaag cgcgcgacca tgtcgcgtgc gatctccgtc
     1141 gcgcgctcga ggtcgttctc ggaccccgtg gacggctcgc cgaagatcat ctcctcggcc
     1201 gcgaggccgg ccatcgacgt cacgagctgg cgcgtcagct ggtagcgggt gagtagcgcg
     1261 gagtcgtcct cggtctcgat cccggtcgcc gccacgttct tgctacgggc caggatcgtg
     1321 acgcggtgca cgtcgtcgcc gcggccgagg gcagccgaca cgagcgcgtg cgcggcctcg
     1381 tggtacgagg cgcggccccg ctcctcgtcg ctgagcacct tgccgcggcg cttggggccg
     1441 tggaggactc gctggatcgc ctcttctagg tcggtcgtct cgatggccga cttcgcgtta
     1501 cgcagcgaca gcagcgcggc ctcgttgatc acgttcgcca ggtcggcgcc cgagaagccg
     1561 ggcgtccgct tcgcgatgta gtccatgtcg acgtcggggg cgagcggctt gccctgcgcg
     1621 tggagcatga ggatctcctt gcgcccgtcg tggtccggct ggtcgaccgt gatgtggcgg
     1681 tcgaaacggc cggggcggag caaagcgggg tcgaggatgt cggggcggtt cgtcgccccg
     1741 atgacgacga tgccggccgc gacgtcgaag ccgtccatct cgacgagcat ctggttcagc
     1801 gtctgctcgc gttcgtcgct gccgccgccg ccggagccgc cggccccacg cttgcggccg
     1861 gccgcgtcga gctcgtcgat gaagacgatg gccggtgcga ccgcgcggac gcgggcgaac
     1921 aggtcacgca cacgagcggc gccgacgccg acgagggact cgacgaactc ggcaccggcg
     1981 accgagaaga acggcacgcc ggcctcgccc gcggtagcgc gggcgatcag cgtcttgcca
     2041 cagcccggag gaccgaacag cagcacgccc ttgggcggca ccgcaccgat gtcttcgtaa
     2101 cggtcggggt tcttcaggta gtcgacgacc tcggagagct cgaggacggc ttcctccgcg
     2161 cccccgacgt ccttgtaggt gaccatcccg gaagcgccct tcttggactt gcccttgccg
     2221 atcgagccga actgcatgac gtcaccgatg ccggagccgc caccgcggcc ggccgtgaag
     2281 agcaggccga agaaggcggc gaggatcagc agcggaagca ggaactggga gacgacacgg
     2341 acgatggcct tggccgtctg ggggtcgacc gacacgctcg cgccggccgc cgtcagcgag
     2401 tcgacgaggg ggccgaacgc cgcaccgttc tgcgggagct ggacccagaa cgtgccctcg
     2461 ccttcggggg ccttcgacgg gggctcgggc ttggccgggg gcttcttcgt ggcgtccttg
     2521 ccggcgcccc ccgcggcgcc ctcgccctgt gcgtcgccct cggcgttctc accctggttc
     2581 gcgggccccg tgccaccggc gttcgcatcg tcgcccgcgg cgtcgtcgcc ctgcgcgttc
     2641 tcgcccttcg cgtcctcgcc cttggcagcc tctgtcttct tgggctcgtc ctcgtaaccc
     2701 tcgagcggct tggcctggaa cgtccccacg agctgcgcgt cctcgtcgcg gaactcggcg
     2761 gtcgcgatcc ggttctcctt cgcgagcgcg tcgagctcgt cgaaggagag ctcggtgccc
     2821 ggcgactggg gctcgaggta ggtctgggcc cacgcgaacg cgccgaagag aaggatcagg
     2881 acgaggccga ggacgatcgt gctccgacgg ccgctggcct tcttgcccgc ctgccgcttc
     2941 ctacggcgcc ggacccggta ctgcttgaac tcgcgggcga acttccggcg ccacgcggca
     3001 aatcccccct gtgcctcact gctcactgtc gacgggcctc cttcgagtgt tccgagtcgt
     3061 cgttaccggc ggacgtgctg gactcgcacc ttctcccggg cgagggacac gcagccgaaa
     3121 gccgccgtca tggtcatcgc accgatcatg aagattgctt ctggtggtac gccatcggag
     3181 tccttggagt aactggacat cgcgtactcg gcctctttcc cacccgcgga ttcctgcgcg
     3241 gccgccgcag cgtgctgatg ctgagcgtac acgtaggcca gttgcggctg ctgctgctcc
     3301 tgatgcgcca tcgccgcctg ggcctgggcc tgggcctggc tctgcgggtt cggctgcggg
     3361 ttcgggttgg ggttcgggac cggcgggatc tcgggcggca cgggcggcgg cgggatgaac
     3421 gcaaccggca gcgcgatgaa gggcagcggc ggcggttcct tggccggatc cggcttcggc
     3481 gggaccttgc acaggaccga cacggtcgag acgtcgtctt tctgcccgtc gacgttcgcg
     3541 ccgagctcga gcgtgaaccg cttgcgcgac tcgagctgcg ggcacgagaa cgtcgcgtcg
     3601 aagcccaacg ggttctcgcc gtcggcctcg acgcagccgg tcgggccctc ttcgaccacg
     3661 ggcgagtccg cgggggcctc ccagcagacc ctcggcgcgt cgatctgcga ctcgaccatg
     3721 ccgatgatcg ccggcgccat cagcgacgcc tgacgcgcgc gcagcgggct gatcttgcat
     3781 acgacggggt cgccgtgcgg gatctcgacg tcgccgtcgt cgtcacagtc gacgccgccc
     3841 tcg
//
LOCUS       DASQDO010000233         6763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_451854, whole genome shotgun sequence.
ACCESSION   DASQDO010000233 DASQDO010000000
VERSION     DASQDO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451854"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VEV43_06710"
     CDS             <1..1052
                     /locus_tag="VEV43_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23245.1"
                     /translation="AAGVDPGEADETTLLGVTVRNEGDEPLDSVQVRGLTTKVITSRC
                     KGNPLTPGRYGPLGTPTTLQPGQEQLGFYKFTERCPGEVKATVRATAAGAEEETADTR
                     IKVPDHAYIVGQLTVAYTMSGPLEGFEYQRLTENLRVRAVGERKTLTGLIDEDRRFSI
                     RVPNRALGRYELSVTGNKVTEAVPGRDVVKAVRGKSVQAKGFELGYDCDVPPLGLDFV
                     PESADYGVAPVAPGRPSRLIFTYECRTQRIEFVQLDIFDESPEPDAFEFDECESSDGS
                     TYSAYYVFPLAESRLPLDRGEFELSILTGSGSGSAGSDLRAATVRGVFTDPGRGTLTV
                     THPECDFLNFEADFS"
     gene            1134..2081
                     /locus_tag="VEV43_06715"
     CDS             1134..2081
                     /locus_tag="VEV43_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYP23246.1"
                     /translation="MDADVILEGQPRRRFLESASGTRVEAFGRFEWLLLTSIALIWGS
                     SFVLIDVGLDSFRPGVIAAARVLLGAAALAAVPAARKPVDRSDLPRIAFLGVIWIGIP
                     LSLFPIAQLWIDSSVAGMINGAMPLMTAAWSIALLRRLPGRIQLAGIAIGFLGIVAIS
                     WEQVAGSTATLLGTVLVLLAVTLYGLAANVAVPLQQRYGALPVLLRAQVAALAIVVPF
                     GLTHLPGSSWSWRAALAMVPLGALGTGLAFVLMATLVGRVGGPRGSVAIYFVPVVAMV
                     LGVVVLDETIARSALAGAALVVAGAWLTSRRQKPTPTPV"
     gene            complement(2110..2469)
                     /locus_tag="VEV43_06720"
     CDS             complement(2110..2469)
                     /locus_tag="VEV43_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23247.1"
                     /translation="MSIGKVAIAALVLCLAVGGGLAALGGRDDGKIVEPIDLDDGARR
                     DDDAAELASVEDDGDDDRTRGDDDRTRGNDGTRGGDNTGDGDATDGDDGTGGGSNTDD
                     VVTYDAPAGTDTGGGTT"
     gene            complement(2497..3375)
                     /locus_tag="VEV43_06725"
     CDS             complement(2497..3375)
                     /locus_tag="VEV43_06725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYP23248.1"
                     /translation="MTTAPSWELAEGDEIAPERFALRLLGGGHKYEAYLAYDEDLQAT
                     VVVKLLRPHVVEDASALDVMRAEAAALSRLNHPVLPRCFGIVEDPPRPHLVLEHLEGP
                     RLSTLLRRQRKLALEQVVMLGLQVSSALHYMHRREMVHLDVKPKNIVMSGPPRLIDLS
                     VARSVERARRATEPIGTDAYMAPEQCEPRLMGGMGPEADVWGWGVTMYEAITGVHAFP
                     KVEDATARDERFPQLHLDPLPLPPDTPPLLAALVMGCLEKRPGNRPQQSDIAGILEPL
                     VAALPRRVVLSRLRPR"
     gene            complement(3396..3746)
                     /locus_tag="VEV43_06730"
     CDS             complement(3396..3746)
                     /locus_tag="VEV43_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23249.1"
                     /translation="MLGRAAVAVLAVSVMTGPSAAFASNDDVMLRREEDAAEVVVMES
                     DDDGDTGNTDGNDGDSGTGDSNDATGSGHTSVSRDRDRSRDDKTRDWTRDGGDGTRDR
                     SAGHTNDRTRNDTR"
     gene            3883..5322
                     /locus_tag="VEV43_06735"
     CDS             3883..5322
                     /locus_tag="VEV43_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYP23250.1"
                     /translation="MRTTTSNRALSGIRVRLLGTFILVLLVATAASVVVVRVILVNRA
                     EQGIDAELVQEAREMRRLSTGSDPETGEPFAGRVRRIFEVFLERNIPAPHEAQLTFVG
                     GEPYLRSRAVVPYRLDRDPDLVARWGSLEESDRGVVETPAGPVEYLAVPVRHEGRTEG
                     VFVVAIFHDLRLQDIDPAIAGAAGVGAIVVVIGSLLAWRLADRILGPVKKVTETARGI
                     SESDLRRRIEVPGSDEIGHLAQTFNEMLDRLEHAFTSQRRFLDDASHELRTPITVIQG
                     QLEVLDDDPDERRKTLEIVMDELDRMSRFVNDLLLLARADRPDFLALDTVDVAALSDE
                     VFSKVRSLGDRDWRLEATGRGVIVADRQRVTQALIQLAQNAVQHTAEGSEIALGSSVR
                     DWEARFWVRDSGPGIAVSEQAHVFDRFRRGHGRRGASEGAGLGLSIVKAIAEAHHGRV
                     ELESEPGAGATFTVSIPVDQPESPESENV"
     gene            5319..5981
                     /locus_tag="VEV43_06740"
     CDS             5319..5981
                     /locus_tag="VEV43_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP23251.1"
                     /translation="MKRILLVEDEERITSFLEKGLAANGFTTSVAATGADGLALARTG
                     EFDLVVLDIGLPDKDGFAVLEELRRHDRAMPVVILTARDAVSDTVRGLEGGADDYVTK
                     PFRFEELLARIKARLREDRGPEAGVLTAGDVTLNLKTRRVARGDSTVELSAREFALAE
                     LFFRHPGQVMTREQLLSNVWGYDYDPGSNIVDVYVGYLRKKVGDDVIKTVRGMGYRLE
                     SS"
     gene            6330..>6763
                     /locus_tag="VEV43_06745"
     CDS             6330..>6763
                     /locus_tag="VEV43_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23252.1"
                     /translation="MALAAMAVPSAPTALANHPANTCLDLEPEVDSNPTGTAHTITAT
                     VRTIAGAGQGSFGTCTGPAPTFSGGTVNIDFEVSGPGDPNEDGPTPETPDLTCNVGAQ
                     LGASCTVSYTSTRASRTATDVPEQEAGADEIRGWIDHDGGDPA"
BASE COUNT          994 a         2369 c         2308 g         1092 t
ORIGIN      
        1 ccgcggcggg ggtcgatccc ggcgaagccg acgagaccac gctcctcggg gtcacggtcc
       61 gcaacgaagg ggacgagcca ctcgacagcg tccaggtgcg tgggctgacc accaaggtga
      121 tcacgtcgag atgcaagggc aatccgttga cgcccggacg ctacggcccc ctgggtacac
      181 cgacgacgct tcagcccggc caggagcagc tcgggttcta caagttcacg gaaaggtgtc
      241 ccggcgaggt caaggcgacc gttcgcgcca cagcggccgg tgccgaagaa gagaccgccg
      301 acacacgcat caaggttccg gaccacgctt acatcgtcgg tcagctcacc gttgcgtaca
      361 cgatgagcgg gcccctggag gggttcgaat accaacgact caccgagaac ctgcgggtcc
      421 gcgccgtagg tgagaggaag acgctcaccg gcctcatcga cgaggaccgc aggttcagca
      481 tcagggtgcc gaaccgcgcc ctgggccgct acgagctgag cgtgacggga aacaaggtga
      541 ccgaggcggt tccggggcgc gacgtcgtga aggcagttcg cgggaagtcg gtccaagcga
      601 agggcttcga gctcggctac gactgcgacg tgccgccgct cggcctggat ttcgtcccgg
      661 agagcgccga ctacggagtc gccccggtcg cgccgggaag accctcccgt ttgatattca
      721 cttacgaatg ccggacgcag cggatcgagt tcgtccagct cgacatcttc gacgagagtc
      781 ctgagcctga tgctttcgag ttcgacgaat gtgagagcag cgacggctcg acgtattcgg
      841 cttattacgt cttcccgctg gccgagtcgc gcttgccctt ggatcgcggg gagttcgagc
      901 tctccatcct caccggctcc ggttcgggtt cggcgggctc ggacctacga gcggcgaccg
      961 tgaggggcgt cttcaccgac ccggggagag gcaccttgac ggtgacgcat cccgagtgcg
     1021 acttcctcaa cttcgaggcg gacttcagct gacggcgacg gtaccggttc gtcccgctcg
     1081 tcagatcgcc ggtgtgcatc tcacggaatc ccgtgcccta acgttctaag ggcatggacg
     1141 cagacgtcat cctcgaagga cagccccgca gacgctttct cgaaagcgcc tccgggacgc
     1201 gcgtcgaggc gttcgggcgc ttcgagtggc tgctgctcac gtcgatcgct ctcatctggg
     1261 gctcttcgtt cgtcctgatc gacgtcggcc tcgactcgtt ccgcccgggc gtcatcgccg
     1321 ccgcccgggt gctgctgggc gccgcggcac tggcggcggt ccccgcggcc cgcaaacccg
     1381 tcgacaggag tgacctcccg cgcatcgcct tcctgggcgt gatctggatc gggataccgc
     1441 tgtcgctgtt cccgatcgcg cagctgtgga tcgactcgtc ggtcgccggg atgatcaacg
     1501 gggcgatgcc actgatgacc gcggcgtggt cgatcgctct cttgcgccgg ctccccgggc
     1561 gcatccagct cgcgggcatc gcgatcgggt tcctggggat cgtcgccatc tcgtgggagc
     1621 aggtcgcagg ttcgaccgcg accttgctgg ggaccgtgct cgtgctcctg gcggtcaccc
     1681 tctacggcct ggcggcgaac gtcgccgtgc cgctgcagca gcgctacggg gccctgccgg
     1741 tgctgttgcg cgcgcaggtc gccgcgctcg cgatcgtcgt cccgttcggc ctcacccatc
     1801 tgccggggtc gagctggtcg tggcgagcgg cgctcgcgat ggtcccgctg ggagcgctcg
     1861 ggaccgggct cgcgttcgtg ctgatggcca cgctggtggg ccgggtcggc ggcccccgcg
     1921 gctcggtcgc catctacttc gtgccggtcg tcgcgatggt gctcggcgtc gtggtgctcg
     1981 acgagacgat cgcgcgcagc gccctggccg gggcggcgct cgtagtcgcg ggggcctggc
     2041 tcacgtcgcg gagacagaag ccaacgccca cgccggtcta ggaagctccc gggactccgt
     2101 ccgagggaat caggtcgtcc ctccgccggt gtcggtcccc gccggagcgt cgtaggtgac
     2161 gacgtcgtcg gtgttgctac cgccgccggt cccgtcgtct ccgtccgtcg cgtcgccgtc
     2221 gccggtgttg tcgccgccgc gagtcccgtc gttgccccgc gtgcggtcgt cgtcgccgcg
     2281 cgtgcggtcg tcgtcaccgt cgtcttcgac gctcgccagc tcggccgcgt cgtcgtcgcg
     2341 ccgggccccg tcgtcgaggt ctatcggctc gacgatcttc ccgtcgtccc tgccgccgag
     2401 ggcggccaga ccgccgccga cggcgagaca caggacgagt gcagcaatcg caaccttccc
     2461 aatgctcatg gcgtctcgtc tcctcgatct agtcggtcac cgcgggcgca gcctgctgag
     2521 cacgacacgt cgcggcagcg ccgctacgag tggctcgagg atccccgcga tgtcggattg
     2581 ctgcgggcgg ttgcccgggc gcttctccag gcagcccatc acgagagcgg cgagaagtgg
     2641 aggcgtgtcc gggggcaggg gcagcgggtc gagatgcagc tgggggaaac gctcgtcccg
     2701 cgcggtcgcg tcctcgacct tcgggaacgc gtggacgccc gtgatcgcct cgtacatcgt
     2761 cacgccccaa ccccacacgt cggcctccgg acccatgccc cccatcagcc gcggctcgca
     2821 ttgctccggc gccatgtacg cgtcggtacc gatcggttcc gtggcgcgcc gcgcgcgctc
     2881 gacgctgcgc gccaccgaca ggtcgatcag ccgcggcgga ccgctcatga cgatgttctt
     2941 cggcttgacg tcgaggtgca ccatctcgcg ccggtgcatg tagtgcagcg cggagctcac
     3001 ctgcagcccg agcatcacga cctgctcgag cgccagcttc ctctgcctgc ggaggagggt
     3061 cgagagccgc ggcccctcga ggtgctccag gacgaggtgg ggtcgcggag ggtcctcgac
     3121 gatgccgaag caccgcggca gcaccgggtg gttgagacgc gacagcgcgg cggcctcggc
     3181 ccgcatcacg tcgagcgcgg aggcgtcttc gactacgtgc ggtctcagca gcttgaccac
     3241 caccgtggcc tgcaggtctt cgtcgtaagc gagatacgcc tcgtacttgt gccctcctcc
     3301 gagcagccgg agggcgaacc gctcgggcgc gatctcgtct ccttcggcga gctcccagct
     3361 tggtgccgtc gtcaccgtgg tcctccgtct tctcgtcagc gggtgtcgtt gcgcgtgcgg
     3421 tcgttcgtgt gccccgcgct gcggtcgcgc gtgccgtcac cgccgtcgcg ggtccaatcc
     3481 cgggtcttgt cgtcgcggct ccggtcgcga tcgcggctca ccgatgtgtg gccgctgccg
     3541 gtcgcgtcgt tgctgtctcc ggtgccgctg tcgccgtcgt tcccgtcggt gttgcccgtg
     3601 tcgccgtcgt cgtcgctctc catcacgacg acctccgctg cgtcttcctc acgccgcagc
     3661 atcacgtcgt cgttcgacgc gaacgccgcc gacggacccg tcatcacgct cactgccagc
     3721 actgcaaccg ctgcacgtcc gagcatcacg tacctcctgt tgtcgtcgtc tgccctcgac
     3781 ttcgaggatg ctctccgcgc atgagaggcg gatgaggcgg acgtgagaga actctcatct
     3841 tcgtctcacg tacgtcccac gcgttcggac gacaatggcg acgtgagaac gaccacctct
     3901 aacagggcgc tctcgggcat ccgcgtgcgt ctgctcggga ccttcatcct cgtgctgctc
     3961 gtcgccacgg cggcgtcggt ggtggtcgtg cgcgtgatcc tcgtcaaccg cgccgagcag
     4021 ggcatcgacg cggagctcgt ccaggaagcc cgtgagatgc gacgtctctc gaccgggagc
     4081 gaccccgaga ccggggagcc gttcgccgga cgcgtgcgcc ggatcttcga ggtcttcctc
     4141 gagcgcaaca tcccggcccc ccacgaagcg cagctgacgt tcgtcggcgg cgagccgtac
     4201 ctgcgcagcc gcgccgtcgt cccctaccgg ctcgaccggg accccgatct cgtcgcccgc
     4261 tggggctccc tcgaggagag cgaccgcggc gtcgtcgaga cgccggccgg gcccgtcgag
     4321 tacctggcgg tccccgtccg gcacgaaggg cggaccgaag gcgtcttcgt cgtcgcgatc
     4381 ttccacgacc tgaggctcca ggacatcgac ccggcgatcg cgggggcagc cggcgtaggc
     4441 gcgatcgtgg tggtgatcgg gtcgctgctg gcatggaggc tcgccgaccg catcctcggg
     4501 ccggtcaaga aggtgaccga gaccgcgcgc ggcatctccg aatccgacct caggcgccgg
     4561 atcgaggtcc ccggaagcga cgagatcggt catctcgcgc agaccttcaa cgagatgctc
     4621 gaccgcctgg agcacgcctt cacgagtcag aggcgcttcc tcgacgacgc cagccacgag
     4681 ctgcgcacgc cgataaccgt gatccaggga cagctcgagg tcctcgacga cgaccccgac
     4741 gaacggcgca agaccctcga gatcgtgatg gacgagctcg atcgcatgag ccgcttcgtc
     4801 aacgacctgc tgctgctcgc cagggccgac cgtcccgact tcctcgcgct ggacaccgtc
     4861 gacgtcgccg ctctctccga cgaggtcttc tccaaggtcc ggtcgctggg cgaccgggac
     4921 tggaggctcg aggcgacggg cagaggcgtg atcgtcgccg accgccagcg cgtgacccag
     4981 gccctcatcc agctcgctca gaacgcggtc cagcacaccg ccgagggaag tgagatcgcg
     5041 ctcgggtcct cggtccgcga ctgggaggcc cgcttctggg tgcgtgactc cggccccggg
     5101 atagcggtga gcgagcaggc tcacgtcttc gaccgcttcc gaagggggca cggacgacgg
     5161 ggcgcgtccg agggggccgg gctcggcctc tcgatcgtca aggcgatcgc ggaggcgcac
     5221 cacggaaggg tcgagctgga gagcgagccc ggggcgggcg cgacctttac cgtgtcgatc
     5281 cccgtggacc agcccgagtc accggaaagt gagaacgtat gaagcggatc ctcttggtcg
     5341 aggacgaaga gcgcatcacc tcgttcctcg agaaggggct ggccgcgaac ggtttcacca
     5401 cctcggtcgc ggcgaccggc gccgacgggc tggcgcttgc acgcaccggc gagttcgacc
     5461 tcgtcgtcct cgacataggc ctcccggaca aggacgggtt cgcggtgctg gaggagctgc
     5521 gccggcacga ccgcgcgatg cctgtcgtca tcctgaccgc ccgcgacgcg gtgtcggaca
     5581 cggtccgcgg cctcgagggc ggggcggacg actacgtgac gaagccgttc cggttcgaag
     5641 agctcctcgc ccgcatcaag gcgcggctgc gcgaggaccg gggcccggag gccggcgtcc
     5701 tcaccgccgg cgacgtcacg ctgaacctca agacgagaag ggtcgcgcgc ggcgactcga
     5761 ccgtcgagct cagcgcgcgc gagttcgcgc tggccgagct gttcttccgg caccccgggc
     5821 aggtcatgac gcgcgagcag ctcctcagca acgtctgggg ctacgactac gaccccggct
     5881 cgaacatcgt cgacgtctac gtcggctacc tgcgcaagaa ggtgggggac gacgtcatca
     5941 agacggtccg gggcatgggc tatcgcttgg aaagcagcta gacgcccttg atcgccgggg
     6001 tgcccccgaa gcggtctgcc cctgaagcga acggcgcggc ccgcccgtga cagagggatg
     6061 acaggagccc gtcactcact agtcatccaa gcccgcatct gggggaaatt tcccgtcaaa
     6121 ccccatatcc acggggctaa cccgtcgcta tcgtgactac tcgcatggcg gccgtcatcg
     6181 gcccgccgga gctacggggg ccgatccgtc tttccgtccc tcgtcgctgt tccaggtgcc
     6241 agtggggagt cacgagtggg ggcaggagga cgatgacgtt ccgtagaggt attcgcgcgc
     6301 gcagacttca cgcgacggcg gtagccgtcg tggcattggc ggcaatggcg gtcccgtccg
     6361 ccccgaccgc tctggcgaac cacccggcga acacgtgcct cgacctcgag cccgaggtcg
     6421 acagcaaccc gaccgggacc gcccacacga tcaccgcgac cgttcggact atcgccgggg
     6481 cgggacaggg ttcgttcggc acctgcaccg gaccggcgcc gacgttcagc ggcgggaccg
     6541 tcaacatcga cttcgaggtc tccggccccg gcgaccccaa cgaggacggg ccgacgcccg
     6601 agacccccga cctgacctgc aacgtcgggg cccagctcgg ggcgtcctgc acggtctctt
     6661 acacgagcac gcgggcgagc cgcacggcga ccgacgttcc cgagcaggaa gccggcgccg
     6721 acgagatccg cgggtggatc gaccacgacg ggggagaccc ggc
//
LOCUS       DASQDO010000234         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_758237, whole genome shotgun sequence.
ACCESSION   DASQDO010000234 DASQDO010000000
VERSION     DASQDO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758237"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VEV43_06750"
     CDS             <1..287
                     /locus_tag="VEV43_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23253.1"
                     /translation="QGGGGYLVVHDHPGNRWASYGPWELTLGPRRRDEREERRTKTFL
                     KGKILLDLRAAATLRSYFSIDELLHVIDKSVGQRDVVEAVAAKTLDLPQV"
     gene            334..>1846
                     /locus_tag="VEV43_06755"
     CDS             334..>1846
                     /locus_tag="VEV43_06755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HYP23254.1"
                     /translation="MSEMPEESADRMEASEPPPRRKGTSTSNFGVGRRESHDASGFYA
                     RFGPLPEVSTDRQIAKLDEVDRIYLGDAKDMSHVASSSVALVVTSPPYFAGKEYEEAL
                     GEGHVPATYVDYLKDLEMVFAECVRVLEPGGRIAVNVANLGRRPYRSLSADVIGLLQD
                     RLGLLLRGEIIWQKGKGAAGSCAWGSFQSAQNPVLRDISERVIIASKGRFDRALTRRE
                     RQRRDLPYEASMSKEAFMEATLDVWEFPSERATRVGHPAPFPVELPKRLIDLYTYYGD
                     VVLDPFMGSGTTAVAALRADRHYLGYEIDSSYIEIATKRIREEQGELDRLKRAGLTRE
                     VRLGVDRNEPEDDEPFQARAVRQGKRAKELAREVLEHCGFRDIVQDHRFMSGVEINFS
                     AIDRRGRTWYFDVSGAFTSSRPGLRRTDTLWKALGKAAVLKEAGLASHRLVFLSTDLP
                     PKGSSGDAALRAVRGKAFHDAIEMLSDAGQDRLRRYGLGEGYEEPLGDLLSPEE"
BASE COUNT          330 a          587 c          634 g          295 t
ORIGIN      
        1 ggcagggcgg cggcggctac ctggtggtgc acgaccatcc gggcaaccgc tgggcgagct
       61 acggcccgtg ggagctgacg ctcggccccc gccgccgcga cgaacgcgag gaacgacgga
      121 cgaagacgtt cctgaagggg aagatcctgc tcgacctgag agcggccgcg acgctgcgca
      181 gctacttctc gatagacgag ctgctgcacg tgatcgacaa gtcggtgggg cagcgagacg
      241 tggtcgaagc cgtagcggcg aagaccctgg acctgcccca ggtctgaggc cttcgcgaaa
      301 ttgtcacagc ccgccgatag cgtcctacct aggatgagcg agatgccgga ggagagcgcg
      361 gaccgcatgg aggcatccga gccacccccg cggcggaagg ggacgagcac ctccaacttc
      421 ggcgtcggcc ggcgggaaag ccacgatgct tccggcttct acgctcggtt cggaccgctc
      481 cccgaggtct cgaccgaccg gcagatcgcc aagctcgacg aggtcgaccg gatctatctc
      541 ggggatgcga aagacatgtc gcacgtcgcc tcgtcgtcgg tcgcgctcgt cgtgacgtcg
      601 ccgccctatt tcgccggcaa ggaatacgag gaggccctcg gcgagggcca cgttccggcc
      661 acctacgtcg actacctcaa ggacctcgag atggtcttcg ccgaatgtgt gcgagtgctc
      721 gagcccggtg gacgcatcgc ggtgaacgtg gcaaacctcg gccgccggcc ctacaggtct
      781 ctttcggcag acgtcatcgg cctgctccaa gaccgtctcg gtctcctgtt gcggggcgag
      841 atcatctggc agaaggggaa gggggccgcg gggtcgtgtg cctggggctc tttccagagc
      901 gcgcagaacc cggtgctccg cgacatctcg gagcgcgtga tcatcgcgtc caagggacga
      961 ttcgacaggg ctctgacgcg tcgtgagcgg cagcggcgcg acctcccgta cgaggcctcg
     1021 atgtcgaaag aggctttcat ggaggcgaca ctcgacgtct gggagttccc gtccgagcgc
     1081 gccacacggg taggacaccc ggcgcctttc ccggtcgagt tgcccaagcg tctcatcgac
     1141 ctctacacgt actacggaga cgtcgtgctc gacccgttca tggggtccgg gacgacggcg
     1201 gtcgcagcgc tgcgcgcaga caggcactac ctcggttacg agatcgacag ctcctatatc
     1261 gagatcgcca ccaagcggat tcgtgaggag cagggcgagc tcgaccgctt gaagcgcgcc
     1321 gggctcaccc gcgaggtgag gctgggcgtc gatcgcaacg agcctgaaga cgacgagccg
     1381 ttccaagccc gcgccgtccg tcagggaaag cgcgcgaagg agctagcccg cgaggtgctc
     1441 gagcactgtg ggttccggga catcgtgcag gatcaccggt tcatgagtgg cgtcgagatc
     1501 aacttctcgg cgatcgaccg cagagggcgt acctggtact tcgacgtgtc cggcgcgttc
     1561 acgagctctc gtcctgggct tcggagaacg gacacattgt ggaaggccct ggggaaggcc
     1621 gcggtcctga aagaggccgg cctcgcctca caccggctgg tcttcctctc gaccgatctt
     1681 cctccgaagg ggtcgtcagg agacgcggca ctccgggccg tacgcggaaa ggctttccac
     1741 gatgcgatcg agatgctctc ggacgcggga caagatcgac tgcgccggta cggtctgggt
     1801 gaaggctacg aggaacccct cggggatctc ctgtccccgg aggagt
//
LOCUS       DASQDO010000235         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_635720, whole genome shotgun sequence.
ACCESSION   DASQDO010000235 DASQDO010000000
VERSION     DASQDO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635720"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3191
                     /locus_tag="VEV43_06760"
     CDS             <1..>3191
                     /locus_tag="VEV43_06760"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase early set domain-containing
                     protein"
                     /protein_id="HYP23255.1"
                     /translation="EGTSVLLPLLPPDYEPRVLICGGKTAWRIHLGQGPQVWKKLDRG
                     ELEGIERIHSCAVLLPTGRVFVSGGITRRDEPGPPGAALIPEVYDPGIDWDENEYDHG
                     PFSSDSTGSWEALSGEPAEVARGYHSVALLLPDGRVWTAGSTDTAGLIAGSGVGSIAE
                     GGSTLTLSSGEFGASAEGKEILVLGAGTPDDETNEPTDLRAVISDVVSATEATLDRAA
                     GAGVSDAGVWWGWIDESRVEIYSPGYVGAANRPVVSTISSATYEETFTVTLENPQAIE
                     RVALIRCGTVTHAFDSDQRYVALEFVQSGDDETLTVTAPPSPQIAPPGNYMLWVVGGN
                     ERVAARAPFIRIADQEAVFGANKSNFSLPEVTAMAEAGDGEARFADALLLDFRGFLLH
                     EIGDPLPSPEFELSFEAPLAGMPPGLTLEAKAPVTQTDDPDRAQHVVYPIDVVFSSDD
                     AFESFTDEQIGVVQVRARVGERSFDLRLVLMRHSTPFMTDVRPDLQNPYWLSTDLRVF
                     KMHPGDEKPSGDPSAPVAHGSDPFAFLDDLLGAYRQNPSAGSHPFDDLSENESLEMFP
                     TDEEGPVFNYAVARVRYFAPDDHPAPGVRVFFRIFTTVGTAVEYDTATSYRREGLRES
                     ARPLLGKEGASIVSVPFFAEERVTPGGAMGSQPDDRNVQGLEGQAPQQYVGYFGCWLD
                     NNQPDPQFPRFPLDDGPFTDVFFPVLSIQELMAGLHQCLVAEIHYWPVGLEADPILAH
                     ASPATSDKLAQRNLAFGDVANPGVVDATRTFHHSFELKAPAFAQPLDLSPRVNAGPEA
                     VRTSPDASRGRLDPDELILWAPGLPDGSTVELYLPTVDAGRLLGLVERSPLGAARFEV
                     RDLHTVAVELRPVVFVPVPGGANENLEGLISVTLPKGIVKEEVFEVVVQQWSGKSRRV
                     VGSFELSIHVDSSDALLPRDMKRLSLLRHIAKSMPPKDRWYRVFDRWIDRAAERVRAF
                     GGDPDDALPSPGGPGGTRPGSPDGGGRWVEGKIARLVYDCFGDFAGFVLDLCPGERRF
                     ASSEAAVESLARRACADRSRVRVYPHGDRSDRF"
BASE COUNT          484 a         1091 c         1096 g          520 t
ORIGIN      
        1 gaggggacgt cggtccttct cccgctactc ccgcccgact acgagccgcg cgtcctcata
       61 tgcggtggga agaccgcatg gaggatccac ctcggacagg gcccacaggt gtggaagaag
      121 ctcgatcgcg gcgagctcga ggggatcgag cggatccact cgtgtgcggt cctgttgccc
      181 accgggcgcg tgttcgtgtc ggggggcatc acgcggcgcg acgaaccggg ccctccgggc
      241 gcggcgttga tcccggaggt ctacgacccc ggcatcgact gggacgagaa cgaatacgac
      301 cacgggccgt tcagctcgga ctcgacgggg tcgtgggagg ccttgtcggg cgagcccgcg
      361 gaggtcgcgc gcgggtacca ctccgtggcg ctgttgctac ccgacgggag ggtgtggacg
      421 gcgggctcga ccgataccgc cgggctgatc gcgggatccg gcgtggggag catcgccgag
      481 ggcgggtcca cgctgaccct ctcttccggg gagttcggtg cgtcggccga gggcaaagag
      541 atcctggtgc tcggcgcggg cacgccggac gacgagacga acgagccgac ggacctccgg
      601 gcggtgatct ccgacgtcgt ctcggcgacc gaggccacgc tcgaccgggc cgccggcgcg
      661 ggcgtgagcg acgccggcgt ctggtgggga tggatcgacg agtcgcgggt cgagatctac
      721 agcccgggct acgtcggcgc cgccaaccgc ccggtcgtga gcacgatctc gtcggcgacg
      781 tacgaagaga cgttcaccgt gacgctcgag aatccacagg cgatcgagcg agtagcgctg
      841 atccggtgcg ggacggtcac tcacgccttc gacagcgacc agcgctacgt cgccttggag
      901 ttcgtccaga gcggcgacga cgagacgttg acggtgacgg cccccccgag cccgcagatc
      961 gctccgcccg gcaactacat gctgtgggtg gtcggcggga acgaacgggt tgctgcgcgc
     1021 gcgccgttca tccgcatcgc cgaccaagaa gccgtcttcg gcgcgaacaa gagcaacttc
     1081 tccctccccg aggtgacggc gatggcggag gccggcgacg gggaagctcg cttcgccgac
     1141 gcgctcctgc tcgacttccg gggcttcttg ctgcacgaga tcggcgaccc gcttccctcg
     1201 cccgagttcg agctctcgtt cgaagcgccg ctcgcgggga tgccgcccgg gctaacgctg
     1261 gaagccaaag cgccggtcac gcagaccgac gaccccgacc gcgcccagca cgtcgtctac
     1321 ccgatagacg tcgtcttctc gtcggacgac gcgttcgaga gcttcaccga cgaacagata
     1381 ggcgtcgtcc aggtccgggc ccgggtcggc gagcggtcct tcgatctccg tctcgtgttg
     1441 atgcggcatt cgacgccgtt catgaccgac gtccgcccgg acctccagaa cccttactgg
     1501 ctgagcaccg acttgcgcgt cttcaagatg cacccgggcg acgagaagcc gtccggcgac
     1561 ccgagcgctc cggtcgccca cgggtcggac cccttcgcct tcctggacga tctgctgggt
     1621 gcctatcggc agaatccgtc cgccggatcc catcccttcg acgacctgtc ggagaacgag
     1681 tccctggaga tgttcccgac cgacgaagaa gggcccgtct tcaactacgc ggtcgcgagg
     1741 gtccgctact tcgctccgga cgaccacccc gcgccggggg taagggtctt cttcaggata
     1801 ttcacgacgg tcggcactgc cgtcgagtac gacacggcta cgtcgtacag acgcgaaggg
     1861 ctgcgcgaat ctgcaagacc actgttgggc aaggagggcg cctcgatcgt ctccgtgccc
     1921 ttcttcgccg aagaacgcgt tacgcccggc ggcgcgatgg ggtctcagcc ggacgatcga
     1981 aacgtgcagg gcctcgaggg ccaggccccg cagcagtacg tcggctactt cggatgctgg
     2041 ctcgacaaca accagccgga tccgcagttc cctcgtttcc ctctggacga cgggcccttc
     2101 acggacgtct tcttccccgt cttgtcgatc caggagctga tggccggact gcaccagtgt
     2161 ctcgtcgcgg agatccacta ctggccggtg gggttggagg ccgatccgat actcgcccac
     2221 gcgagccctg cgacgagcga caagctggct caacgcaacc tcgccttcgg cgacgtcgcc
     2281 aaccccgggg tggtcgacgc gacccgtacg ttccaccaca gcttcgagct gaaggctccg
     2341 gcgttcgccc agccgctcga tctctcgccg cgcgtcaacg ccggcccgga ggccgttcgg
     2401 acgtcgcccg acgcgtcgcg cgggcgcctt gatcccgacg agctcatcct gtgggcaccc
     2461 ggcctccccg acggctcgac cgtcgagctc tacctcccga cggtcgacgc cggacgcctt
     2521 ctggggctgg tcgaacgctc gccgctcggc gcggcgcggt tcgaggtgcg cgaccttcac
     2581 accgtcgccg tcgagctccg ccccgtggtc ttcgtgccgg ttccgggcgg cgcgaacgag
     2641 aacctcgagg ggttgatctc ggtgaccctc cccaagggga tcgtcaaaga ggaggtcttc
     2701 gaggtagtcg tccagcagtg gtcggggaag tcccggaggg tcgtcggctc gttcgagctg
     2761 tccatccacg tcgattcttc ggacgcgctg ctgccgcgag acatgaagcg cctgtcgctc
     2821 ctccgtcaca tcgcgaagtc gatgcctccg aaggatcggt ggtaccgggt cttcgaccgc
     2881 tggatcgacc gggcggccga gcgcgtaagg gctttcggag gagatcccga cgacgcgctt
     2941 ccttctccgg gggggcccgg tgggacgcgc ccgggatcgc ccgacggcgg gggccgctgg
     3001 gtcgagggga agatcgcgag gctcgtgtac gactgcttcg gagacttcgc cgggttcgtc
     3061 ttggacctct gtccgggcga acgccggttc gcgagcagcg aggcggcggt cgagtccctg
     3121 gcccgacgag cgtgtgccga tcgttcccga gtgcgcgtct atccgcacgg cgaccgctcg
     3181 gatcgattct g
//
LOCUS       DASQDO010000236         4853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_574234, whole genome shotgun sequence.
ACCESSION   DASQDO010000236 DASQDO010000000
VERSION     DASQDO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574234"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEV43_06765"
     CDS             <1..213
                     /locus_tag="VEV43_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23256.1"
                     /translation="DSIQEFERAVLFQLLLTSGDVDENATADVIAAVEKAQAERPKRV
                     SEDAPRRRKNPKARQHRRSRRPPRRR"
     gene            249..2168
                     /locus_tag="VEV43_06770"
     CDS             249..2168
                     /locus_tag="VEV43_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYP23257.1"
                     /translation="MTAPDRYPASSRAVLVALARGVVGREYEPGLLPRMLEVADLLPT
                     TSQRRQLVTALATLDSRAGALALTGRPVPVSWLTATEAEALLQRWKSSSLPPLRDLAN
                     VVISLVLSTFYGTVPRQWERIGYSGPLTPAPDVPKRLDPIAIEEDTVTACDVVVVGSG
                     AGGGCTAAQLAAAGLDVVVLEKGSYVSESDFHHREYEATRQMYLYGLTLATSDLGCRI
                     IAGATLGGGTVVNYTTSFKTPPHVLEQWRRTSGIDAFVSGEFVESLDQVSERLGVNTD
                     SSAAGRRDEVLEEGLKKLGWHVDALPRAVRGCTQDEACGYCGFGCRVGAKQSTMVTYL
                     QDAADAGARIFTGADVRRVSITDGRATGIVAVCGRHRLTIEARAVVVAGGAIETPALL
                     LRSGLGGQVGRNLRLHPGTAAFGVFDDDVNMWEGTLQSRYSAELRGADGGYGPIFETV
                     PVHPGAGSAALPWVSSRHHLDLMSRFGKLSLCAVLARDETAGRVRIARDGGPRVDYKL
                     TPSDERRIADGVVAAGKVMEAAGATEVFSQHHSWVSYRPGPPGAHERWADETRRAGYR
                     AGLTFFSYHQMGSCRMGVDPAASAVDADNESHEVRNLFVTDASAFPTASGVNPMLSIY
                     GIANRAGRRLAERLS"
     gene            2332..3348
                     /locus_tag="VEV43_06775"
     CDS             2332..3348
                     /locus_tag="VEV43_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23258.1"
                     /translation="MRQALYDLVEAADLNGLLRAVDGMCAAREWDELLDLADACDDAL
                     DRGKQLWPISAHVDYRIALEAPGEYAASVLTPEIGRFAHGPLTEVAASTHSWDELVAH
                     LEDPVTASFVAQERVLRGETLDTDERAHAGLVDVPLRIAPWEPPYALATFRSNLVEVQ
                     EPWEPQAPWEEADPGAPVKLDEDEIVDALLELVTPWTNESNGAARAVVVDGDAAGAIG
                     SLTYGPVRLASIGVDEAFRQMAWAAASGGAHGRRRGAALGRFSAWYTTALLADLPWPV
                     DAAELGAAAERLRWFAWDEEAVGGAPQEGWVLRIAVEDPDEGWAAALGATDVLTEAGE
                     DADG"
     gene            3341..3889
                     /locus_tag="VEV43_06780"
     CDS             3341..3889
                     /locus_tag="VEV43_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23259.1"
                     /translation="MDESPELSVLVHELGGMLSSVQGFAHIASENPGHPDRERFIKLA
                     AQEARKAAQVVKDIHLVRSFDRGRIEPAPPPADLGELVPGGAGTAVRADVAKVRGLLQ
                     RCVDAAPAPPEVVDAGERVEVRIAIAPAADLARRIESLDAPYPDMVTYAILRRLLRYW
                     DGDVTLATAGDWTYAVLTFVRA"
     gene            complement(3886..4602)
                     /locus_tag="VEV43_06785"
     CDS             complement(3886..4602)
                     /locus_tag="VEV43_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HYP23260.1"
                     /translation="MPQLPDGTTITWLGHSTFLVETAGARILIDPWVQGNPACPDEMK
                     DVGDLDAMLITHGHFDHISDAVEIAKSKGPQTVCIAETAHWLGSKGVENVIDMNKGGT
                     VEAAGCRVHMVHAVHSCGITDGDTIVYGGEAAGYVLELPGGFSIYHAGDTAVFSDMAL
                     IGRLLAPDLALLPIGDHYTMGPRSAAEAVRLLGVKAVVPMHFGTFPVLTGTPEDLRRE
                     AADVKGLEVVDLRPGGSLGG"
     gene            4672..>4853
                     /locus_tag="VEV43_06790"
     CDS             4672..>4853
                     /locus_tag="VEV43_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23261.1"
                     /translation="MLVHVLGALLAASVGGGPHALRPDEICLTERLCARQPVYCLMDE
                     TRCDDPIDVCLIEKCD"
BASE COUNT          664 a         1680 c         1799 g          710 t
ORIGIN      
        1 gactcgatcc aggagttcga gcgggccgtg ctgttccagc tcctgctcac gagcggcgac
       61 gtcgacgaga acgcgaccgc cgacgtgatc gccgcggtcg agaaagctca ggccgagcgg
      121 ccgaagcgcg tctccgagga cgccccgcgc cgccgcaaga accccaaggc ccgccagcat
      181 cgccgttcgc gccgcccgcc gcgccgccgg tagcagccac ccccgcgcgg gtgtaggaac
      241 tcctactagt gaccgcgcca gaccgctatc ccgcatcgag ccgcgccgtg ctggttgcgc
      301 tggcgcgcgg ggtcgtcggg cgtgagtacg agcccggcct cctcccgcgg atgctcgagg
      361 tcgccgacct gttgccgacc acgtcgcaac ggcgccagct cgtgacggcg ctggcgacgc
      421 tcgactcgcg ggccggagcg ctcgcgctga ccgggcgtcc tgtcccggtc tcgtggctga
      481 cggcgacgga agccgaagcc ctgctgcaga ggtggaagtc gagctcgctc ccgccgctgc
      541 gggacctcgc caacgtcgtg atctccctcg tcctctcgac gttctacggg accgtgccgc
      601 gccagtggga gcggatcggc tactcgggcc cgctgacccc ggcccccgac gtgccgaagc
      661 ggctcgaccc gatcgcgatc gaagaagaca ccgtgaccgc gtgcgacgtg gtcgtcgtgg
      721 gctcgggagc cggcggtggc tgcacggccg cgcagctcgc ggcagccggg ctcgacgtcg
      781 tcgtcctgga gaaggggtcg tacgtctcag agtccgactt ccaccaccgc gagtacgagg
      841 cgacgcgcca gatgtacctc tacggcctca cgctcgcgac cagcgacctc ggctgccgca
      901 tcatcgccgg cgcgacgctc ggcggcggca ccgtcgtcaa ctacacgacg tcgttcaaga
      961 cgcccccgca cgtgctcgag caatggcgac ggacctcggg catcgatgcg ttcgtctccg
     1021 gggagttcgt cgagtcgctg gaccaggtga gcgaacggct cggcgtcaac accgactcga
     1081 gcgccgcggg ccgccgggac gaggtgctcg aggagggcct gaagaagctg ggttggcacg
     1141 tcgacgcgct gccgcgtgcg gtgcgagggt gcacgcagga cgaggcatgt gggtactgcg
     1201 ggttcggctg ccgcgtcggc gccaagcagt cgacgatggt gacctacctg caggacgccg
     1261 ccgatgcggg ggcgcgcata ttcaccggag ccgacgtgcg ccgggtgagc atcaccgacg
     1321 gccgcgcgac ggggatcgtc gccgtctgcg gccgacaccg tctgacgatc gaggcacgcg
     1381 cggtcgtcgt cgccggcggt gccatcgaga ccccggccct gttgctgcgg tcgggtctag
     1441 gggggcaggt ggggcgaaac ctgcgcctgc acccgggcac ggctgcgttc ggcgtcttcg
     1501 acgacgacgt gaacatgtgg gaggggacgc tgcagtcgcg gtactcggcc gagctacgcg
     1561 gcgccgacgg ggggtacggg ccgatcttcg agaccgtgcc cgttcatccc ggagcggggt
     1621 cggccgcgct cccgtgggtg tcgtctcggc atcacctcga cctcatgagc cgcttcggca
     1681 agctgtcgct gtgcgcggtg ctggcgcgcg acgagacggc cggacgggtc cggatcgcgc
     1741 gcgacggggg gccgcgcgtc gactacaagc tcacgccctc cgacgagcgc aggatcgccg
     1801 acggcgtagt cgcggcaggg aaggtgatgg aggcggccgg cgcgaccgag gtgttctcgc
     1861 agcaccactc gtgggtttcc taccgccccg gcccccccgg cgcgcacgaa cggtgggcgg
     1921 acgagacgcg gcgcgccggc tatcgcgcag ggctcacgtt cttctcgtac caccagatgg
     1981 ggtcgtgccg gatgggtgtg gacccggcgg cctccgcggt cgacgcggac aacgagtcgc
     2041 acgaggtccg caacctcttc gtcaccgacg cgtccgcgtt cccgacggcg tcgggcgtga
     2101 accccatgct gtcgatctac gggatcgcga accgggccgg gcgccggctc gccgagcggc
     2161 tctcctagcc ggctgagtcc ttgtcctctc tcgggtggtc gaacgcgatg tgctggtcca
     2221 ggtccccacg tgacgcgaac ttgaggtcgc agtgagggca ttgcaggacg tcgtccacgg
     2281 tcggctcctc tcgctcggcg atcaccccca tgctcgcacg gcggggccga tgtgagacag
     2341 gccctctacg acctcgtcga ggcggcggac ctcaacgggc tcctgcgggc cgtcgacggg
     2401 atgtgcgcgg cccgagagtg ggacgagctg ctcgacctcg ccgacgcctg tgacgacgcg
     2461 ctcgaccgcg gcaagcagct gtggccgatc agcgcgcacg tcgactaccg catcgctctc
     2521 gaggcgccgg gggagtacgc ggcttccgtc ctcacgccgg agatcgggag gttcgcgcac
     2581 ggccccctga cggaggtggc ggcgtcgacg cattcgtggg acgagctggt ggcacatctc
     2641 gaggatcccg tgacagcgtc gttcgtcgcg caggagcgcg tgctgcgcgg cgagacgctc
     2701 gacaccgacg agcgcgcgca tgccgggctc gtggacgtgc cgctcaggat cgcgccgtgg
     2761 gagccgccgt acgcgctcgc gacgttccgt tcgaacctcg tcgaggtcca ggagccgtgg
     2821 gagccgcagg caccgtggga ggaggccgac ccgggggccc ccgtgaagct cgacgaggac
     2881 gagatcgtgg acgcgctgct cgagctcgtc accccgtgga cgaacgagtc gaacggagcg
     2941 gcccgcgcgg tcgtcgtcga cggggacgcg gccggggcga tcggctcgct cacgtacggg
     3001 ccggtgcgtc tcgcgtccat cggcgtggac gaggcgttcc ggcagatggc ctgggccgcg
     3061 gccagcggcg gggctcacgg tcgccgcagg ggggccgcgc tcggccgttt ctccgcctgg
     3121 tacaccaccg cgctcctcgc cgacctgccg tggccggtgg acgcggccga gctcggcgcg
     3181 gctgcggaac gcctgcgctg gttcgcgtgg gacgaagagg ccgtcggcgg cgcgccacag
     3241 gagggctggg tgctgcggat cgcggtcgag gaccccgacg aaggctgggc tgcggccctc
     3301 ggcgcgaccg acgtgctgac ggaggcgggg gaggacgccg atggatgaga gccccgagct
     3361 gtcggtgctc gtccacgagc tcggcgggat gctgtcgagc gtccagggct tcgcccacat
     3421 cgcgtccgag aaccccggcc atcccgaccg cgagaggttc atcaagctcg cggcgcagga
     3481 ggcccgcaag gccgcgcagg tcgtcaagga catccatctc gtcaggtcgt tcgaccgggg
     3541 ccggatcgag cctgcgccgc cgccggccga cctcggcgag ctggtcccgg gcggcgccgg
     3601 cacggcggtc cgcgccgacg tggcgaaggt acgcggcctg ctgcagcgtt gcgtcgacgc
     3661 ggcccccgcg ccacccgagg tcgtcgacgc cggggagcgc gtagaggtgc ggatcgcgat
     3721 cgcgcccgcg gcggacctcg cccggaggat cgagtcgcta gacgcgccct atccggacat
     3781 ggtgacttac gcgatcctgc gccggctctt gcggtactgg gacggcgacg tgacgcttgc
     3841 gaccgcgggc gactggacgt acgccgtcct gacgttcgtc cgggcctaac cccccagcga
     3901 gccgccaggc cgcaggtcga cgacctcgag ccctttcacg tccgcggcct cgcgccggag
     3961 gtcctccggg gtcccggtca ggaccgggaa cgtcccgaag tgcatcggca cgacggcctt
     4021 cacgccgagc aggcgcactg cctccgcggc cgagcgcggc cccatcgtgt agtggtcgcc
     4081 gatggggagc agcgcgaggt cgggcgcgag caggcggccg atcaacgcca tgtcggagaa
     4141 caccgcggtg tcgcctgcgt ggtagatgct gaagccgccg gggagctcga ggacgtagcc
     4201 cgcggcctcg ccgccgtaga cgatcgtgtc gccgtcggtg atgccgcacg agtgcaccgc
     4261 gtggaccatg tggacgcggc agccggcggc ctcgaccgtg ccgcccttgt tcatgtcgat
     4321 cacgttctcg acgcccttcg agccgagcca gtgtgcggtc tcggcgatgc agacggtctg
     4381 ggggcccttg ctcttggcga tctcgacggc gtcggagatg tggtcgaagt ggccgtgtgt
     4441 gatcagcatc gcgtcgaggt cgccgacgtc cttcatctcg tccgggcagg ccgggttccc
     4501 ctgtacccac gggtcgatca ggatgcgggc gccggccgtc tcgacgagga acgtcgagtg
     4561 gcccagccag gtgatcgtgg tcccgtccgg aagctgtggc atccctgacc ctcctcttgt
     4621 cggtttttcg ggggccgata ctaggagccg ccgggggcgt agcctcgttg catgctcgtg
     4681 cacgtgctgg gggctctgct ggcggcttct gtgggaggcg gtccacacgc gctccgcccg
     4741 gacgagatct gtctgacgga acgcctctgc gcgcggcagc ccgtctactg cctgatggac
     4801 gagacgcgct gcgacgaccc tatcgatgtc tgcctgatcg agaagtgcga cga
//
LOCUS       DASQDO010000237         7483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_85064, whole genome shotgun sequence.
ACCESSION   DASQDO010000237 DASQDO010000000
VERSION     DASQDO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85064"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VEV43_06795"
     CDS             complement(<1..942)
                     /locus_tag="VEV43_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HYP23262.1"
                     /translation="MPKSSSSASGNLVIRGAREHNLKNVHLELPRDALIVFTGISGSG
                     KSSLAFDTIYAEGQRRYVESLSSYARQFLGQMDKPEVDFIEGLSPAISIDQKSASRNP
                     RSTVGTITEIYDYLRLLFARIGRPHCHNCGRAVARQTPEQIVDQILELPEGTRFQVLA
                     PIVRSRKGEFTNLFEDLARRGYARARVDGEVRALTEKIRLDRYFQHDVDVIVDRLVLK
                     EGIVSRLTDSIETALQLAEGTAAIDLVDDAGEDILFSQHFACTFCGLSFDELAPRNFS
                     FNSPYGACERCDGLGTHLEVDADLVIPNPDLSIEEGAI"
     gene            1174..2322
                     /locus_tag="VEV43_06800"
     CDS             1174..2322
                     /locus_tag="VEV43_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYP23263.1"
                     /translation="MTDLAAYRSEFPVAGERTYLISASLGPLSNRARAAALEHLDLWS
                     RLGPEELWFDHGMPKLEQCRALFAQLIGADPDETAIVPSVSAGLSQIGTCLDFDKRPK
                     VVLTDMDFPTNHYVWRAAEKAGAKVEVVRSPDGVTVPAEDVIARIDEQTAVVNLNRVL
                     FESSWIMDAPAIVAAAHDNGALVVVDDFHGSGIVPIDVHELGADVLLTGALKWLCGGQ
                     GIAFLYCRRDLIERMEPRVVGWFGTKDFFGFDRTGLRLRDDARRFETGTLTLPQAWTA
                     AAGLEIVLEVGVDEIRRRNQELTEGIVARADDAGIEVTSPRDKARRGGLVRLGVPGGP
                     ERAKEVLHALFERDVVLDKRGAALRVSPHFFNDERDLDRCFAALRELL"
     gene            2540..4621
                     /locus_tag="VEV43_06805"
     CDS             2540..4621
                     /locus_tag="VEV43_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP23264.1"
                     /translation="MKIAFGISWNDSKKGQFLAAVGLSLGLLFASVPGMAQQMALATS
                     EMVDVIVRELPGAGSEPEQMVEDLGGEVGRHIGLIDGFTATVPKAGLPALESVRKVHS
                     VTRDQRVHLLHAVDGYDGATAPGSMYQARLALKADDTWHDGFTGKGVDVALIDSGVLP
                     VEGLTYPGKVVNGPDLSLESGSEPHRYLDTFGHGTHMAGIIAGRDAAVTETGKRSKTD
                     HDNFQGIAPDARILNVKVAASDGATDVSQVIAAIDWVVQHRNTGGLNVRVLNLSFGTD
                     GTQSYLVDPLTYAAEVAWHKGIVVVVAAGNSQFGNNALNNPAYDPYVIAVGAADTKGT
                     PYDNTDDVVPDWSARGTATRGPDVVAPGKSVASLRAPGSNLDAAHPEGRVNARLFKGS
                     GTSQAAAMVSGAAAVLLQHRPNLTPDQVKALLTSTATKLPAADAQEQGAGLVNVRAAQ
                     KAATPLLATQTWPRATGTGSLEKARGTVHLVMKGPDKACLQNYEETKAAAATAAAATP
                     APQPVVETVESTVTDTVTDVTETSGAVVEATEPATDDSAEPATAPAEETAGTAPAEET
                     AGTAPAESSAPAVTTEPAPATTTEPAPVTPAQTAEEACPPIDHVLAGEQDIFGNAWNG
                     ASWSGASWSGQTWSGGQWNGASWSGASWSGASWSGQTWTGQTWSGQTWCGPTWSGQTW
                     SGQTWSGQTWS"
     gene            4690..6747
                     /locus_tag="VEV43_06810"
     CDS             4690..6747
                     /locus_tag="VEV43_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23265.1"
                     /translation="MKAITGAVSVEQERGVLPPLLRLSGTTRVWLLNIAIAGLAALLV
                     AALGAAGVDSVDNPFEIPWWAIAGLFFLAEVYVVHLQFRREAHSFSLSEIPLVLGLYF
                     LAPPVLVLAQLVGSGTALVLHRRQSPLKLVFNLAHLSLEATFASLLFHWVVGRGDPAG
                     LTGWIGTVVAVALTTVLAAVMITIAISLSEGKLHLETLTEGLGLGVMVGMTNTCIALV
                     AATLMWERPEAAWLLLVPVATVFFAYRAYTSERQKHKSLESLYESSRVVQGSLQIEAT
                     ISELLSQARKMFQAEVAQITFFPTPGEAAFRSSMGPGEEGYLMRELELDPRDGVWARV
                     ASEGEALLIPKPILNENIRKHFEARGMRDAIVAPLHGGDGVIGTFAVGSRLGDVSTFD
                     EADLKLFETLANHASVSLENARLVGRLEESLAHLTEMNRLKDDFVAAVSHELRTPLTS
                     IQGYIKTLLRPNVEFSPDDQRSFIEAVDRQSERLRRLIEDLLVVSRIESDSDKLLVTP
                     VLLQKLAHHVVDEFRARTGNRSFGFTFDSLLEVDTDETKVHQVVSNFVDNALKYAPDG
                     TTITIGGRVEGEHIVVSVADEGPGIPDIEQARIFDRFYQIDQSSTRAAGGTGLGLYIC
                     KKLAEAIGGDVMLEKSDETGSTFALWIPLSPPRDAVRRQSFPSLTIGRPPATGTEPAF
                     SIR"
     gene            6774..7193
                     /locus_tag="VEV43_06815"
     CDS             6774..7193
                     /locus_tag="VEV43_06815"
                     /inference="COORDINATES: protein motif:HMM:NF042533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HYP23266.1"
                     /translation="MRALAIVAAALLLTGCDDPGVIEAKGGNVRLEVIPAEVAAGPFP
                     EHVLVNDSKQTVVYGHSYDLERWVAGKWKDVGFRCAFSGEAISLDPGDRSPAESLGGC
                     ADEMPLEPGIYRVSKDVDVAEGDATDDRLLVETFTVS"
     gene            complement(7186..7467)
                     /locus_tag="VEV43_06820"
     CDS             complement(7186..7467)
                     /locus_tag="VEV43_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23267.1"
                     /translation="MLLWIGLLQPVSAVAFTLDGILMGASDMRYLAGAMAACSACFVP
                     VALVSVGAGWGTAGLTAGLAVWLALRSLLTGWRFANGRWLRAGIARAVS"
BASE COUNT         1176 a         2652 c         2559 g         1096 t
ORIGIN      
        1 gatcgcgccc tcctcgattg acaggtccgg gttcgggatc acgaggtcgg cgtcgacctc
       61 gaggtgcgtc ccgagcccgt cgcagcgctc gcacgcgccg tagggcgagt tgaacgagaa
      121 gttcctcggc gccagctcgt cgaaggagag cccgcagaac gtgcacgcga agtgctggct
      181 aaacaggatg tcctcgccgg cgtcgtcgac gaggtcgatc gccgcggtcc cctcggccag
      241 ctgcagagcg gtctcgatcg agtcggtcag ccggctgacg atcccctcct tcagcacgag
      301 gcggtcgacg atcacgtcga cgtcgtgctg gaagtagcgg tcgagccgga tcttctcggt
      361 gagcgcgcgc acctcgccgt cgacccgggc gcgggcgtag ccccgccggg cgaggtcctc
      421 gaacaggttc gtgaactcgc ccttgcggct ccggacgatc ggggcgagga cctggaagcg
      481 cgtcccctcc ggcagctcga ggatctggtc gacgatctgc tcgggggtct gccgggcgac
      541 cgcacggccg cagttgtggc agtgcggacg gccgatccgg gcgaacagca ggcggaggta
      601 gtcgtagatc tcggtgatcg tcccgacggt cgaacggggg ttccgggacg ccgacttctg
      661 gtcgatcgag atcgccggcg agaggccctc gatgaagtcg acctcgggct tgtccatctg
      721 gccgaggaac tggcgggcat aggacgacag cgactcgacg taccggcgct ggccctcggc
      781 gtagatggtg tcgaaggcca acgacgactt ccccgagccg gagatccccg tgaagacgat
      841 cagcgcgtcc cggggcagct cgaggtgaac gttcttcagg ttgtgctcgc gggccccccg
      901 gatgacgagg ttccccgagg ccgacgagga tgatttcggc acgatgggat cgtaaacgac
      961 gagcgtgtaa ctctaatttc cgttttcgcg ggccggcccc ctgacgaccc tcctcgcgga
     1021 ggccgtggta cgcgtggtcg cgctggtacg cgtggttggg aacccaacct ctcgtaccac
     1081 gccaaccaca cgtaccagac gaaccacggc cctccggaag aagaggtgtt cgtcgacgcc
     1141 ggggccgccc ggcccggcga taccctggcg ggcgtgaccg acctcgccgc ctatcgctcc
     1201 gagttccccg tcgccgggga gaggacgtat ctcatctccg cgtcgctcgg ccccctttcc
     1261 aaccgcgccc gggccgccgc cctcgagcac ctcgacctgt ggtcccggct cgggcccgag
     1321 gagctgtggt tcgaccacgg catgcccaag ctcgagcagt gccgggcgct gttcgcgcag
     1381 ctgatcgggg cggatcccga cgagacggcg atcgtccctt cggtctcggc cgggctctcg
     1441 cagatcggga cgtgcctcga cttcgacaag aggccgaagg tcgtcctcac cgacatggac
     1501 ttcccgacga accactacgt gtggcgtgcg gctgagaagg cgggggcaaa ggtcgaggtc
     1561 gtgcgctccc ccgacggcgt gaccgtgccg gccgaggacg tgatcgcacg catcgacgag
     1621 cagaccgccg tcgtcaacct caaccgcgtc ctgttcgagt cgtcctggat catggacgcg
     1681 cccgcgatcg tcgcggccgc ccacgacaac ggcgcgctcg tcgtggtcga cgacttccac
     1741 ggctcgggca tcgtcccgat cgacgtgcac gagctgggcg ccgacgtgct gctcaccggc
     1801 gcgttgaagt ggctgtgcgg cggtcagggc atcgcgttcc tctactgccg tcgcgacctg
     1861 atcgagcgca tggagccccg ggtcgtcggc tggttcggca cgaaggactt cttcgggttc
     1921 gaccgcaccg ggctgaggct gcgggacgac gcccgccgtt tcgagaccgg gacgttgacg
     1981 ctcccgcagg cgtggaccgc ggccgcgggc ctcgagatcg tgctcgaggt aggcgtggac
     2041 gagatccgcc gccgcaacca ggagctgacg gagggcatcg tcgcccgcgc cgacgacgcg
     2101 gggatcgagg tgacgtctcc gcgcgacaag gcgaggcgag gcggcctcgt gcggctcggg
     2161 gtccccggag ggcccgagcg cgcgaaggag gtcctccacg ccctgttcga gcgcgatgtc
     2221 gtgctcgaca agaggggggc cgcgctgcgc gtctcgccgc acttcttcaa cgacgaacgc
     2281 gacctcgatc gctgcttcgc cgcgctgcgc gaactgctct aaaccgccga tcccgtcgcg
     2341 gtctagtccc ccatcccggc gcgaaaccgg acacatcgca gcccctgact agtccgttcg
     2401 aagtgcgcgc gagatgacgt gaaaggacca tggggatttc ccgatcgggt gccgatgaag
     2461 ggactgcggg ggcaggagtt tcaccgggag ggtggatttt cgtgttttgc cggtgcagtg
     2521 cccaggaggg ggttcgcgta tgaagatagc gttcgggatc agctggaacg attccaagaa
     2581 gggccaattc ctagccgccg taggcctgtc gttgggtctg cttttcgcgt cggtgcccgg
     2641 gatggctcag cagatggcgc tggcgacgtc ggagatggtc gacgtcatcg tccgcgagct
     2701 ccccggtgcg ggctccgagc ccgagcagat ggtcgaggac ctcggtggcg aggtcggccg
     2761 ccatatcggc ttgatcgacg gattcactgc gacggtcccc aaggccggtc tccccgcact
     2821 cgagtcggtc cgcaaggtcc actcggtcac gcgcgaccag cgcgtccacc tgctgcacgc
     2881 ggtcgacggg tacgacggcg ccaccgcgcc gggctcgatg taccaggccc gcctcgcgct
     2941 caaggccgac gacacctggc acgacgggtt caccggcaag ggcgtcgacg tcgcgctcat
     3001 cgactcgggc gtcctgcccg tcgagggcct gacgtacccg ggcaaggtcg tgaacggccc
     3061 ggacctctcc ctcgagtccg gctccgagcc gcaccgctac ctcgacacgt tcgggcacgg
     3121 cacgcacatg gccggcatca tcgccgggcg cgacgcggcc gtcaccgaga ccggcaagcg
     3181 gtccaagacc gaccacgaca acttccaggg catcgcgccc gacgctcgca tcctgaacgt
     3241 caaggtcgcg gcctcggacg gcgccacgga cgtgtcgcag gtcatcgcgg cgatcgactg
     3301 ggtcgtccag caccgcaaca ccggggggct gaacgtccgg gtgctgaacc tctccttcgg
     3361 cacggacggc acgcagagct acctggtcga cccgctgacg tacgcggccg aggtcgcgtg
     3421 gcacaagggg atcgtcgtcg tcgtcgccgc cgggaactcg cagttcggca acaacgcgct
     3481 gaacaacccg gcctacgacc cgtacgtgat cgccgtcggc gccgccgaca ccaaggggac
     3541 gccgtacgac aacaccgacg acgtcgtgcc cgactggtcg gcccgtggca ccgccacgcg
     3601 cggccccgac gtcgtggccc ccggcaagtc cgtcgcaagc ctccgggccc ccgggtccaa
     3661 cctcgacgca gcgcaccccg agggccgcgt gaacgcccgc ctgttcaagg gcagcggcac
     3721 gtcccaggcg gccgcgatgg tgtcgggcgc ggctgcggtc ctcctgcagc accgtccgaa
     3781 cctcacgccg gaccaggtca aggcgctcct gacgagcacc gccacgaagc tacctgccgc
     3841 cgacgctcag gagcagggcg cgggcctcgt gaacgtgagg gccgcgcaga aggcggcgac
     3901 gcctctcctg gcgacccaga cgtggccccg cgccaccggg accggctctc tcgagaaggc
     3961 ccgcggcacc gtccacctcg tcatgaaggg cccggacaag gcctgtctcc agaattacga
     4021 ggagacgaag gctgccgcgg ccaccgcggc tgccgcgacc cccgctccgc agccggtcgt
     4081 cgagacggtc gagagcacgg tcacggacac ggtcaccgac gtgaccgaga cctcgggcgc
     4141 cgtcgtcgag gcgacggagc cggcgacgga cgactctgcc gagccggcga cggctccggc
     4201 cgaggagacc gcgggtacgg ctccggccga ggagaccgcg ggtacggctc cggccgaaag
     4261 ctcggctccg gccgtcacga ccgagccggc accggccacc acgaccgagc cggcacctgt
     4321 gaccccggcc cagaccgccg aggaggcgtg tcccccgatc gaccacgtgc tcgcaggcga
     4381 gcaggacatc ttcggcaacg cctggaacgg cgccagctgg tcgggtgcga gctggagcgg
     4441 ccagacctgg agcggtggcc agtggaacgg cgccagctgg tccggcgcaa gctggtcggg
     4501 tgcctcctgg agcggccaga cctggaccgg acagacgtgg tccggccaga cgtggtgcgg
     4561 cccgacctgg tccggtcaga cgtggtcggg acagacctgg tccggtcaga cctggtccta
     4621 ggcaagaccc gcagaaaaca ccgcacgagt tgaaggcaga caccgcaacg tccgactaga
     4681 gaaagacgaa tgaaggcgat caccggggca gtttcggtcg agcaggagcg gggggtcctc
     4741 cccccgctcc ttcgcctgtc cggcaccacc cgcgtctggc tgctgaacat cgccatcgcg
     4801 ggcctggccg cgctgctcgt cgccgcactg ggagcggccg gcgtcgactc ggtcgacaac
     4861 ccgttcgaga tcccgtggtg ggcgatcgca gggctcttct tcctcgcgga ggtctacgtc
     4921 gttcacttgc agttccgccg cgaggcgcac tcgttctcgc tcagcgagat cccgctcgtc
     4981 ctcgggctct acttcctcgc tcccccggtc ctcgtgctcg cgcagctcgt cggatccggc
     5041 accgcgctgg tgctccaccg ccggcagtcg ccgctgaagc tcgtcttcaa cctcgcgcac
     5101 ctgtcgctcg aggcgacctt cgcctctctc ctgttccact gggtggtcgg acgcggcgac
     5161 cccgccgggc tcaccggatg gatcgggacg gtggtcgcgg tcgcgctgac gaccgtgctg
     5221 gccgccgtga tgatcacgat cgcgatctcg ctcagcgagg gcaagctgca cctcgagacg
     5281 ctcaccgagg gtctcggcct cggggtcatg gtcggtatga cgaacacgtg catcgctctc
     5341 gtcgccgcga cgctcatgtg ggagcggccc gaggcggcgt ggctcctgct ggtccccgtc
     5401 gccaccgtct tcttcgccta ccgcgcctac acgtccgagc gccagaagca caagagcctc
     5461 gagtccctct acgagtcgag ccgcgtcgtc cagggctcgt tgcagatcga ggcgaccatc
     5521 tccgagctcc tgtcacaggc gcgcaagatg ttccaggcgg aggtcgcgca gatcacgttc
     5581 ttccccacgc ccggcgaggc cgcgttccgc agctcgatgg ggccgggaga agagggctac
     5641 ctgatgcgcg agctcgagct cgacccgcgc gacggcgtct gggcccgggt cgcgtccgag
     5701 ggcgaggccc tgctgatccc gaagccgatc ctcaacgaga acatccgcaa gcacttcgag
     5761 gcccgcggca tgcgggacgc gatcgtcgcc ccgctccacg gcggggacgg ggtcatcggg
     5821 accttcgccg tcgggagccg gctcggcgac gtcagcacgt tcgacgaggc cgacctgaag
     5881 ctcttcgaga ccctcgcgaa ccacgccagc gtctcgctcg agaacgcccg cctcgtcggc
     5941 cggctcgagg agtcgctcgc gcacctcacg gagatgaacc gcctcaagga cgacttcgtc
     6001 gccgcggtgt cgcacgagct gcgtaccccg ctcacgtcca tccagggcta catcaagacg
     6061 ctgctccggc cgaacgtcga gttctcaccc gacgaccagc gctcgttcat cgaggcggtc
     6121 gaccgtcaga gcgagagact gcgacggctg atcgaggatc tgctcgtggt ctcgcgcatc
     6181 gagtccgaca gcgacaagct cctcgtcacc cccgtgctgc tccagaagct cgcgcaccac
     6241 gtcgtcgacg agttccgggc ccgcacgggc aaccgttcgt tcggcttcac cttcgacagc
     6301 ctgctcgagg tcgacaccga cgagacgaag gtccatcagg tggtctcgaa cttcgtcgac
     6361 aacgcgctca agtacgcgcc ggacgggacc acgatcacga tcgggggccg cgtcgagggc
     6421 gagcacatcg tggtgtcggt cgcggacgag gggccgggga tcccggacat cgagcaggcg
     6481 cggatcttcg accgcttcta ccagatcgac cagtcgtcga cccgggccgc aggcggcacc
     6541 gggctgggcc tctacatctg caagaagctc gccgaggcca tcggcggcga cgtcatgctc
     6601 gagaagtcgg acgagaccgg ctcgacgttc gcgttgtgga tcccgctcag cccgccgcgc
     6661 gacgcggtgc gccgccagag cttcccgtct ctgacgatcg ggcgtccgcc ggcgacgggc
     6721 accgagcccg ctttctccat ccgttagccg cgcgggggcg tagcctctcg gttatgcggg
     6781 cgctagcgat cgtggccgcg gcgttgctgc tgaccggctg cgacgacccc ggtgtcatcg
     6841 aggcgaaggg cggcaacgtc cggctcgaag tgatcccggc cgaggtcgcc gccgggccgt
     6901 tcccggagca cgtcctcgtg aacgactcga agcagaccgt cgtctacggg cactcttacg
     6961 acctcgagcg atgggtcgcc ggcaagtgga aggacgtcgg cttccgatgc gcgttctccg
     7021 gcgaggcgat ctcgctcgac cccggcgatc gcagcccggc cgagtcgctc ggcggctgcg
     7081 cggacgagat gccgctggag cccggcatct accgggtctc gaaggacgtg gacgtcgccg
     7141 aaggcgacgc gacggacgac cggctgctcg tcgagacgtt caccgtcagc tgacggcgcg
     7201 ggcaattccg gcccgcagcc agcgcccgtt ggcgaagcgc caccccgtca gcagcgagcg
     7261 cagcgccagc cacaccgcca ggcccgcggt caatccggcg gtcccccacc ccgccccgac
     7321 cgagaccagt gcgacgggga cgaagcacgc gctgcacgcg gccatcgccc ccgccagata
     7381 gcgcatgtcc gacgctccca tcaggatgcc gtcgagcgtg aaagcgaccg ccgacaccgg
     7441 ctgcagcagc ccgatccaca gcagcacgcc ggcggccgcg tcg
//
LOCUS       DASQDO010000238         6925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_146480, whole genome shotgun sequence.
ACCESSION   DASQDO010000238 DASQDO010000000
VERSION     DASQDO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146480"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..687)
                     /locus_tag="VEV43_06825"
     CDS             complement(130..687)
                     /locus_tag="VEV43_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23268.1"
                     /translation="MTEWSVAITVGRPAGGDVEAEAGEVVAALGEFAPAVSVGRGDAT
                     IRLALAGNTVRDALSRALVEVRGAVEAVGWPPGVRHVEATEWGEFEKRLEAPALPELV
                     GVTEIAELLGTSRQRASELARSPKFPAPLADLAAGPVWPKPVVARFAEEWERKPGRPR
                     SKTSERLDQIIEDRRAREVEGTRER"
     gene            complement(684..905)
                     /locus_tag="VEV43_06830"
     CDS             complement(684..905)
                     /locus_tag="VEV43_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23269.1"
                     /translation="MGSDWNKLLKEAERQGWTIEPGRKGQSKLVPPDPAKQIVFVHST
                     PSDHRALKNAVAEMRRQGLQWPPRGKGER"
     gene            complement(989..1210)
                     /locus_tag="VEV43_06835"
     CDS             complement(989..1210)
                     /locus_tag="VEV43_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23270.1"
                     /translation="MRIDCSDCQMYQSEHCDDCLVTALLRPQSTSVELEDELDPPLQT
                     LAGAGLIPVLRFRPREAQAPPGPNVSQAG"
     gene            complement(1275..2474)
                     /locus_tag="VEV43_06840"
     CDS             complement(1275..2474)
                     /locus_tag="VEV43_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYP23271.1"
                     /translation="MAIPDAAVPLLVKGLGAYMKGAPALDLPPDLRRLRNFHQKMLMA
                     HSAEIVGLLEDETARGLVLQWLDDAQGLPKKQERAVRLAAERGDGWEEALAELAGGER
                     APAGPDPEQRLRDALQRETDKTAAAKEETKRVKESSRKSLDDERATSKELRRELEAAR
                     RELAANAAAAAAAREEAERALADAHKLERRARKDVAAAEAREKEATQQLKAARKDLAA
                     ARRRISELELALEKAAASKRPRAAAATVPSGPRRPLPVPQGRFEDDPLTLDAWLDAPG
                     VHLLVDGYNVTKSPAGFPDLDLPSQRERLIEQLEKLALRKNVPTTVVFDGSDVAPGRS
                     RLARTRVRVEYSRPGEIADDHLMAKLASLPPFPVVLVTGDKELQERAGGEGATIAVAE
                     QLLELAR"
     gene            complement(2477..3658)
                     /locus_tag="VEV43_06845"
     CDS             complement(2477..3658)
                     /locus_tag="VEV43_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYP23272.1"
                     /translation="MSAGPLEGLRVLDLTRLLPGGYATCLLADLGADVVKVEEPGRGD
                     YIRLMPPMAGDVSAGHVALNRNKRSITLNLKTPEGRDVFEDLVPSFDVVVESFRPGVM
                     DRLGIGWDALSRVHPRLVYCAITGYGQDGPRARVAGHDVDYAAYSGVLGITGEPGGRP
                     VVPGVQIGDLAGGGMAAVIAILAALRKRDATGEGDFCDVSMTDGLVSWLSIHAASFFA
                     TGEVPRRGEMHLSGAYPCYRVYPASDGWLAVGALEPQFWAALCSAIDCPDLADDGFAV
                     GERRDAVVAQLEELFSTRSRAEWMEHFGDADVCVAPVNDLAETFADEQVLHREMVVDG
                     SLPDGSAWRHVGIPVKLRGAPGDVSRRPPPDLGEHTDEVLGEAGVDADRLAELRALGA
                     L"
     gene            complement(3655..3867)
                     /locus_tag="VEV43_06850"
     CDS             complement(3655..3867)
                     /locus_tag="VEV43_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23273.1"
                     /translation="MSYRCDACGNKTRFDVVETKRLKSFVHFTLAGEPRVEDEEVLEH
                     RVEKVVCRWCGSTDQIAEVPAAGESA"
     gene            complement(3864..5654)
                     /locus_tag="VEV43_06855"
     CDS             complement(3864..5654)
                     /locus_tag="VEV43_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HYP23274.1"
                     /translation="MVREMAERGPDLPADQRVIEQEQTLQTVREESEVAHALLGLSGA
                     LADVKSVEETMDLAVRIVPALLGASRCFAATWDADSGRFAIHAHYGYDEDEVADRQNL
                     SATSDGLPLLRRALEQRAPIVIADTSAEDPVVAERAAERGVGAYIGLPLVRWGEDLGG
                     LGIEFAEPRDFTSKDHALARGIARQLATAFASARRFNLLADLRTLGLRIGSKLRLAAV
                     VEEVAAGAVQLLSAESAAMYFLDSTQSSLVAAGSYGRKAPVLSDGMARLDLTRDPWRG
                     LLAGEIVVVKDLGEHLGEGAARISAVAAPIQRAEPPMTGAVVLFFSWDPPVGSDEREA
                     LQVLAAQAATALENAQRFERQRSVARSLQEGLLATELPEMPGYESAAVYEPASGEADI
                     GGDFFDVFDLGDGSYAIVVGDVSGKGAEAAAYTAMAKYMLRAFAIRNAQPASVLYHLN
                     NALVTGFGEDRFATLMYALFDPEARKLTVAVGGHPPPLVYRASTDEVEPVALSGSIVG
                     AFVDQAFDQSSFTLGPRDVMLAFTDGLVEARRGDELYGRERVVESLGRHAPGASAETI
                     TQRVFSDAQAYGEINDDTVVFALGCLEERP"
     gene            5732..>6925
                     /locus_tag="VEV43_06860"
     CDS             5732..>6925
                     /locus_tag="VEV43_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HYP23275.1"
                     /translation="MAVAQRTFDQLGRPLAGVTFCVVDLETTGGSPAQCGITEIGALK
                     VRMGERQGSFHTLVQPDEMVPAFIRLLTGITDEMLVDAPPIGTVLPSFLEFVRDSVLV
                     AHNARFDVGFLNAALARDGYPPLANDVVDTATLARKILAGEVRNNKLETLARHLRCAN
                     QPCHRAFADVLATNEVLHHLIERAAGYGVTTLEDLLAMSSTRIDGTFSKIRLAEGLPA
                     SPGVYRFLGSSGQTLYVGKATDLRARVRSYFYGDPRRKMRDLLRETQDVSFETHATTL
                     EAEVAEARAIARETPPYNRRGKGSASWYVKVSLRKRSVAPARSPAADGSAYLGPLPSL
                     KHARTLIDTVRDATGVHRCSSPSSCRGCAFADLGTCAGLDRSAHEDEVRAVVEAFTRD
                     PGIVVR"
BASE COUNT          945 a         2560 c         2381 g         1039 t
ORIGIN      
        1 gcggacctga tctctgcgag gaacgccttg agcggtggct ggtagtcgcc acaggtgcag
       61 gtgtcgcggc cgagcgcggt ggcgatgtcg gcaggcaagc tcagcccgca gtcggggcac
      121 cgttcttcgc tagcgctcac gcgtgccctc cacctctcgc gcgcgccggt cttcgatgat
      181 ctggtcgagg cgttccgacg tcttcgagcg cgggcggccg ggcttccgct cccactcctc
      241 cgcaaaccgc gccacgacgg gcttcggcca gaccgggccg gccgccaggt ctgcgagcgg
      301 tgccgggaac ttcggcgacc gtgcgagctc cgaggcgcgc tggcgcgacg tcccgagcag
      361 ctcggctatc tcggtgacgc cgacgagctc cgggagggcc ggcgcctcga gccttttttc
      421 gaactcgccc cactcggttg cctcgacgtg tctcaccccc ggcggccacc ccacggcttc
      481 cactgcaccg cggacctcca cgagggcacg cgacaacgcg tcgcgaacgg tgtttccggc
      541 gagcgcgaga cggatcgtcg cgtcaccgcg gccgacgctg actgcgggag cgaactcgcc
      601 cagcgccgcg actacttcgc cggcctcggc ctcgacgtcc ccgcccgccg gccggcccac
      661 cgtgatggcg acgctccatt ccgtcatcgt tcgccctttc ctcgcggcgg ccactggagc
      721 ccctgccgtc gcatctcggc gaccgcgttc ttcagtgcgc ggtggtcgga cggcgtcgag
      781 tggacgaaga cgatctgctt ggccgggtcg ggggggacca gcttcgactg ccccttccgt
      841 cccggctcga tcgtccagcc ctgtctctcc gcttccttca ggagcttgtt ccagtcggag
      901 cccaatcgtc cgccttttcc ttgtttctgt caacaattta ccgcagagcg gacgatgtca
      961 agggtttcgt gggcctaaac cctcgcggct aaccggcttg ggacacgttc ggccccggcg
     1021 gggcttgggc ttccctcggc cggaacctca gcaccgggat cagcccggcc cccgccagcg
     1081 tctgtagcgg cgggtccagc tcgtcctcga gctcgaccga ggtcgactgc gggcggagca
     1141 gggccgtcac caggcagtcg tcgcagtgct cgctctggta catctggcaa tcggaacagt
     1201 cgatcctcat ctgcgtgctc ctcccgtgcg ggccaacgtc ctccacacgc taaagagcgg
     1261 gtacgacact tttcctagcg ggccagctcc agcagctgct cggccaccgc gatcgtggcc
     1321 ccctcgcccc ccgcacgctc ctggagctcc ttgtcccccg tcacgaggac gaccgggaac
     1381 gggggcaggg acgcgagctt cgccatcaga tggtcgtcgg cgatctcgcc gggccgggag
     1441 tactcgaccc gcaccctcgt ccgggcgagc cgggaccgcc cgggcgccac gtcggacccg
     1501 tcgaagacga cggtcgtcgg cacgttcttt cggagcgcga gcttctcgag ctgctcgatc
     1561 agccgctcgc gctgggacgg aaggtcgagg tcgggaaacc ccgcgggcga cttcgtcacg
     1621 ttgtagccgt cgacgagcag gtgcacgccg ggagcgtcca gccacgcgtc gagggtcagc
     1681 ggatcgtcct cgaacctccc ttgcggtacc gggagcggcc ggcgcgggcc cgacggaacg
     1741 gtggcggccg cggcacgggg ccgcttcgaa gcagcagcct tctccagcgc gagctcgagc
     1801 tctgagatcc tgcggcgcgc tgcggcgagg tccttgcgcg cggccttcag ctgctgcgtc
     1861 gcctccttct cgcgcgcctc cgccgcggcg acgtccttgc gagcgcgccg ttcgagcttg
     1921 tgcgcgtcgg ccagggcgcg ctccgcctcc tcccgtgccg cggctgccgc cgccgcgttc
     1981 gccgcgagct ccctgcgcgc ggcctccagc tccctgcgca gctccttgct cgtcgcgcgc
     2041 tcgtcgtcga gcgacttgcg cgacgactcc ttgacgcgct tcgtctcctc cttcgcggct
     2101 gcggtcttgt cggtctcgcg ctgcagcgcg tcgcgcagcc gttgctcggg gtcgggcccc
     2161 gcgggcgcgc gctcaccgcc ggcgagctcg gcgagcgcct cctcccaccc gtcgccgcgt
     2221 tcggcagcga gacgcacggc gcgctcctgc ttcttcggaa gcccctgcgc gtcgtccagc
     2281 cactgcagca cgagcccccg cgcggtctcg tcttcgagaa gccccacgat ctcggccgag
     2341 tgcgccatca gcatcttctg gtggaagttg cgcagacgcc tcaggtccgg ggggaggtcg
     2401 agcgcgggcg ctcccttcat gtacgcgccg agccctttca cgagaagggg gaccgctgcg
     2461 tcggggatcg ccacggtcag agcgcgccca gagcgcgcag ctccgcgagc cggtccgcgt
     2521 cgacgccggc ctctccgagc acctcgtcgg tgtgctcgcc gagatcgggc gggggccggc
     2581 gggacacgtc tcccggcgcg ccccgcagct tgaccgggat cccgacgtga cgccacgcgc
     2641 tcccgtccgg cagcgagccg tccacgacca tctcgcgatg caggacctgc tcgtccgcga
     2701 acgtctccgc gagatcgttc accggcgcga cgcagacgtc ggcgtcgccg aagtgttcca
     2761 tccattcggc gcggctgcgc gtcgagaaca gctcttccag ctgcgcgacc acggcgtcgc
     2821 gccgttcccc caccgcgaac ccgtcgtcgg cgaggtcggg acagtcgatg gccgagcaca
     2881 gcgcggccca gaactgcggc tcgagcgctc cgaccgcgag ccacccgtcg gaagccggat
     2941 agacgcggta gcagggatac gcccccgaga ggtgcatctc gccgcggcgc ggcacctcgc
     3001 ccgtggcgaa gaacgaggcc gcgtggatcg agagccacga cacgagcccg tctgtcatcg
     3061 acacgtcgca gaagtcgcct tcgccggtcg cgtcgcgctt gcgcagcgcg gcgaggatcg
     3121 cgatgaccgc ggccatgccc ccgccggcga ggtcgccgat ctgcacgccg gggacgaccg
     3181 gccgcccgcc gggctcgccc gtgatcccca gcacgccgga gtacgcggcg tagtccacgt
     3241 cgtgacctgc gacacgcgct cgcggcccgt cctgtccgta gccggtgatc gcgcagtaga
     3301 cgaggcgggg gtgcacgcgc gacagcgcgt cccagccgat gccgagccgg tccatcaccc
     3361 ccggacggaa ggactcgacg acgacgtcga acgacggcac gaggtcttcg aacacgtcgc
     3421 gcccttccgg cgtcttcagg ttcagcgtga tcgagcgctt gttgcggttc agcgcgacgt
     3481 ggcccgccga gacgtcaccg gccatcgggg gcatcagccg gatgtagtcg ccgcggcccg
     3541 gctcctcgac cttgacgacg tcggcaccga ggtcggcgag gagacacgtg gcgtagcccc
     3601 ccggcaggag ccgcgtgaga tcgagcacgc gcaggccctc gagcgggccc gcgctcacgc
     3661 gctctccccg gcggccggga cctccgctat ctggtcggtc gagccacacc accggcacac
     3721 gaccttctcg acgcggtgct cgaggacctc ttcgtcttcc acgcggggct cgcccgcgag
     3781 cgtgaagtgc acgaagctct tgagcctctt cgtctcgacg acgtcgaaac gcgtcttgtt
     3841 gccgcacgcg tcgcagcggt agctcacggc cgctcctcga ggcagccgag cgcgaacacg
     3901 accgtgtcgt cgttgatctc gccgtacgcc tgcgcgtcgg agaagacccg ctgcgtgatc
     3961 gtctcggcag acgcgccggg cgcgtgccgg cccagcgact cgacgacccg ctcgcgtccg
     4021 tagagctcgt caccgcgccg ggcctcgacg aggccgtcgg tgaacgcgag catgacgtcg
     4081 cgggggccga gcgtgaacga gctctggtcg aacgcctggt cgacgaacgc gccgacgatg
     4141 ctcccggaca gagccacggg ctcgacctcg tcggtcgagg ctcggtacac gagcgggggt
     4201 ggatgcccgc cgacggcgac ggtcagcttg cgggcctcgg ggtcgaacag cgcatacatc
     4261 agcgtcgcga agcgatcctc gccgaagccc gtcaccaggg cgttgttcag gtgatagagc
     4321 accgacgccg gctgggcgtt gcggatcgcg aaggcgcgca gcatgtactt cgccatcgcc
     4381 gtgtacgcag cggcctcggc ccccttcccc gacacgtccc cgacgacgat cgcgtacgac
     4441 ccgtctccga ggtcgaagac gtcgaagaag tcaccaccga tgtcggcctc gccgctcgcg
     4501 ggctcgtaga cggcggccga ctcgtagccg ggcatctcgg ggagctcggt cgcgagcaga
     4561 ccctcttgca ggctgcgcgc gacgctgcgc tgacgctcga agcgttgcgc gttctccagc
     4621 gcggtcgcgg cctgcgccgc gaggacctgc agggcctccc gctcgtcgct ccctaccggc
     4681 ggatcccacg agaagaacag gacgaccgca ccggtcatcg ggggctcggc gcgctggatc
     4741 ggagccgcga ccgccgagat gcgggcggcg ccctcgccga ggtgctcgcc gaggtctttc
     4801 acgacgacga tctctcccgc gagcagcccg cgccacgggt cgcgcgtgag gtccaggcgc
     4861 gccatcccgt ccgagagcac gggggccttg cgaccgtagc tgccggccgc gacgaggctg
     4921 ctctgcgtcg agtccaggaa gtacatcgcc gcggactcgg ccgacaacag ctgcaccgct
     4981 ccggccgcga cctcctccac gaccgccgcg agccggagct tggagccgat gcgcagaccc
     5041 aaggtccgca ggtccgcgag aagattgaag cgccgggcgg acgcgaacgc ggtcgcgagc
     5101 tggcgggcga tcccgcgggc cagcgcgtgg tccttcgagg tgaagtcgcg cggctccgcg
     5161 aactcgatcc cgagcccgcc gaggtcctcg ccccagcgca cgagtgggag gccgatgtac
     5221 gcgcctacgc cgcgctccgc agcacgctcg gcgacgaccg gatcctcggc ggacgtgtcg
     5281 gcgatcacga tcggggcgcg ctgctcgagc gcgcgccgca gcaacgggag cccgtcggac
     5341 gtcgccgaga ggttctggcg gtcggcgacc tcgtcctcgt cgtagccgta gtgcgcgtgg
     5401 atcgcgaagc ggccgctgtc ggcgtcccac gtggccgcga agcacctgct ggcgccgagc
     5461 agcgcaggga cgatccggac ggcgagatcc atggtctcct cgaccgactt cacgtcggcg
     5521 agagcgcccg agagacccag cagcgcgtgc gcgacctcgg actcctcgcg aacggtctgg
     5581 agcgtctgct cctgctcgat cacccgctgg tcggcgggga ggtccggccc cctctcggcc
     5641 atctcgcgca ccatgggcgc cactctagcc gtgggaggca cgggggccgg ggggcttgcc
     5701 atatcgaacg tatgttcgct atcgtgcccc gatggcggtg gcccagagga ccttcgacca
     5761 gctcgggcgg ccgctcgccg gcgtcacgtt ctgcgtggtc gacctcgaga cgacgggcgg
     5821 gtccccggcc cagtgcggga tcaccgagat cggcgccctc aaggtgcgca tgggcgagcg
     5881 ccaggggtcg ttccacacgc tcgtccagcc cgacgagatg gtgcccgcgt tcatccggct
     5941 cctcaccggg atcacggacg agatgctcgt cgacgcgccg ccgatcggga ccgtgctgcc
     6001 gtcgttcctc gagttcgtgc gcgactccgt cctggtcgct cacaacgctc gcttcgacgt
     6061 cgggttcctc aacgccgcgc tcgcgcgcga cggctatccc ccgctggcga acgacgtcgt
     6121 cgacaccgcg acgctcgcgc gcaagatcct cgccggcgag gtccgcaaca acaagctcga
     6181 gacgctggcg cgtcacctgc gttgtgcgaa ccagccgtgt caccgcgcgt tcgccgacgt
     6241 gctcgcgacg aacgaagtgc tgcaccacct catcgagcgc gccgcgggtt acggcgtcac
     6301 tacgctcgag gacctcctcg cgatgtcctc gacgcgcatc gacgggacct tctcgaagat
     6361 ccggctggcc gagggcctgc ccgcgtcgcc gggtgtgtac cggttcctcg ggtcgtccgg
     6421 gcagacgctc tacgtcggca aggcgacgga tctcagggcc cgcgtgcggt cgtacttcta
     6481 cggagacccg cggcgcaaga tgcgtgacct gctgcgcgag acgcaggacg tgtcgttcga
     6541 gacgcatgcg acgacgctcg aggcggaggt ggccgaggcc cgggcgatcg cgcgcgagac
     6601 gccgccgtac aaccggcgcg gcaagggctc ggcgagctgg tacgtcaagg tgtcgttgcg
     6661 gaagcgctcg gtcgcgcctg cccgctcccc cgccgccgac ggaagcgcct atctcggccc
     6721 cctgccgtcg ctcaagcatg cgcgcacact gatcgacacg gtgcgggacg ccacgggtgt
     6781 gcaccggtgc tcgtcgccgt cgtcgtgccg cggctgcgcg ttcgccgacc tgggtacgtg
     6841 cgccgggctc gaccggtccg cgcacgaaga cgaggtgcgt gcggtcgtcg aagcgttcac
     6901 gcgtgacccc gggatcgtcg tgcgt
//
LOCUS       DASQDO010000239         5195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_85164, whole genome shotgun sequence.
ACCESSION   DASQDO010000239 DASQDO010000000
VERSION     DASQDO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85164"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1518)
                     /locus_tag="VEV43_06865"
     CDS             complement(67..1518)
                     /locus_tag="VEV43_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23276.1"
                     /translation="MRRIVLAATAATLAASFAPATAAAAVHLCFGERATIVGTEKKDK
                     LNGTGRRDVIVALGGSDVVHGGEGNDLICLGASDKGGWGEKGFGDRGRDRISGGSGEE
                     TLYGGKGRDVLELDSGGGRAIGGPAGDEMTGGPGRDYFGGERGPGSGQSSSGGGYGYE
                     YAAGEPGSDTFSGRGGNDRFFIGSGNKTVVGGKGIDRLVYWSHERGDFRADLGTGRAR
                     GPGRSRLKGIDEVHAWTPGDVVLRGDGGANELLSVPSLSGANAQLYGGGGDDLLVGQE
                     DWNRRQRGDTDFYGGLGDDTIVGQWTENDERIDELFGGPGDDLIDPRVGARQVSGGPG
                     DDVIEPYWEAPEESSDSSYDGGPGFDTIGWPNSAGVAADLETDHATVSFGEVGTWTYQ
                     ILGIEGVYGSESGRDVLFGDDGPNLLVGGRKGVGYLGTPDGDELDGRGGDDELFGDDG
                     SDELDGGEGVDLLNGGDDVDVCRNGEELEECEG"
     gene            1594..1776
                     /locus_tag="VEV43_06870"
     CDS             1594..1776
                     /locus_tag="VEV43_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23277.1"
                     /translation="MRIKKLFLGAALAMGAVGFMPSPAQAVTCYDAVEPFIGRTCTDC
                     GWVMIGGKPYILFYCD"
     gene            complement(1764..2510)
                     /locus_tag="VEV43_06875"
     CDS             complement(1764..2510)
                     /locus_tag="VEV43_06875"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYP23278.1"
                     /translation="MGVGLDMGPHPLWLLEDLMGDLEITSGMRVLDLGSGLGATSVFL
                     ARELGAEVFAVDLWVPAEDAARTFAEAGVQDRVHALHADARKLPFAPSFFDAVVSIDA
                     FEYFGTDDTYLPYLLDFVKSGGQVGIAVPSTTRELRELGRIPDHVRRCVGWEGVAWHT
                     PEWWRDHWALTGLVEVTSARPQPDAWAKWLLWSKVCRQHNAESDYDVASVIEMLEADG
                     GELLSFALVTARKGAAVAGSPSDVAVDQSQ"
     gene            2692..3165
                     /locus_tag="VEV43_06880"
     CDS             2692..3165
                     /locus_tag="VEV43_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23279.1"
                     /translation="MRGAPLDYEAFYRREFDDVFRGCILMTGSREVALDATQEAFTRA
                     YVRWRRLRRHAWAGGWVMTTALNLCRRQGRVPVPVGKVDSSAAIPEAGPDRVDLQRAL
                     ATLPARQRQATILHYLGDLPVGAVAELMQISDSAVKSHLHAARGALRRTLEVTDA"
     gene            3158..5074
                     /locus_tag="VEV43_06885"
     CDS             3158..5074
                     /locus_tag="VEV43_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23280.1"
                     /translation="MPEDLRELFRREASEIPSAQHDARAVTAAGRRRLRWRRVVTASA
                     LIVAVTAGAGVVRLASREAHPPAAQERLSVLARVPLSSSWSSHSRGVGDLAYGLGKVW
                     ALQGREPTLQRIDPGTNRVEEVLTLANGDNGNFWHVEAGFGRLWLTNPHRDGLLVLDP
                     RSGDVIETVEGVPRALDLVATNEHLWVSVATRDAGIYRVDPETFEVAGPISMGAECCA
                     SGMVEHGGFLWVAHSDVGDQARNPGAPHDDLVFDLTNELRKFDAETLELVDSIRLPGD
                     TYRPGDTVLGGLVAAAGHLWVAHPDGGFVDRVDPASGTVESVDLNGVRPSALTYFRGA
                     VWAWHINGSRVVPIDPSGLEVGPTSDLGEDVSGWPVEGGGSLWTAVQPGNSGDYLLRV
                     GSADGPAPVETPQPPPEQTPPPRGEVVEPVSEREQAQIFVFRALAETGLMKPFSARSY
                     NFASAEDTSRVGDAWRIGLAASDCEPRGEPGSLSFTCTGISGEDPQSGIPMNDTFLTV
                     ELSDGAWTVTGVEGNMPEDDRSRIVGYTAEQRAEPSHWEFPAAEAWPMRSGYMVEMWA
                     LWIGPYPTDARGSVCVVTLLDRAGEPLGEPIRSFEEPPNRAFETTGTARGLDLETIDG
                     DVARVDVDCYQQGS"
     gene            complement(5082..5155)
                     /locus_tag="VEV43_06890"
     tRNA            complement(5082..5155)
                     /locus_tag="VEV43_06890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5119..5121),aa:Leu,seq:taa)
BASE COUNT          779 a         1858 c         1774 g          784 t
ORIGIN      
        1 gtgtcacatg aggaccgcta ttggtgcgcc atgtgacaga gaaacccgaa aacgacaacg
       61 gcccggctag ccctcgcact cctccagctc ttcgccgttc cggcatacgt ccacgtcgtc
      121 gccaccgttc agcaggtcga cgccctcgcc cccgtcgagc tcgtcgctcc cgtcgtctcc
      181 gaacagctcg tcgtcgcccc cgcggccgtc cagctcgtcc ccgtccggcg tccccaggta
      241 cccaacgccc ttcctcccgc cgaccaggag gttcggcccg tcgtcaccga acaagacgtc
      301 ccggccggac tcgctcccgt acacgccctc gatcccgagg atctggtacg tccacgtacc
      361 gacctcgccg aagctcaccg tcgcgtggtc cgtctcgaga tcggccgcaa cgcctgccga
      421 gttgggccac ccgatcgtgt cgaaacccgg ccccccgtcg tacgacgagt ccgacgactc
      481 ctcgggcgcc tcccagtacg gctcgatcac gtcgtccccg ggcccgcccg agacctggcg
      541 cgcccccaca cgcgggtcga tcaggtcgtc gcccggccca ccgaagagct cgtcgatccg
      601 ctcgtcgttc tcggtccact gcccgacgat cgtgtcgtcg ccgaggccgc cgtagaagtc
      661 ggtgtcgccg cgttgccgcc ggttccagtc ctcctgaccc accagcaggt cgtcgccgcc
      721 accgccgtag agctgcgcgt tcgcgccgga gagcgacggc accgacagca gctcgttcgc
      781 tcccccgtca ccgcgcagga cgacgtcgcc gggcgtccat gcgtgcacct cgtcgatccc
      841 cttcagtctg ctccgccccg gccccctcgc gcggcccgtg ccgaggtcgg cacggaagtc
      901 accccgttcg tgcgaccagt agacgagccg gtcgatgccc ttgcctccaa ccaccgtctt
      961 gttgccggaa ccgatgaaga agcggtcgtt cccaccgcgg ccggagaagg tgtcggatcc
     1021 cggctcgccc gcggcgtact cgtagccgta cccgccgccg gagctcgact gcccggaccc
     1081 gggccccctc tcaccaccga agtagtcgcg ccccggcccc ccggtcatct cgtcgcccgc
     1141 gggcccgccg atcgcccggc caccaccgga gtcgagctcg agcacgtccc ggcccttgcc
     1201 gccgtagaga gtctcttcac ccgagccgcc cgagatccgg tcgcgcccac ggtcgccgaa
     1261 ccccttctcg ccccatccgc ccttgtcgct cgcgccgagg cagatgaggt cgttgccctc
     1321 tccgccgtgg acgacgtcgg accccccgag cgccacgatc acatcgcgtc ggccggtgcc
     1381 gttcaacttg tccttcttct cggtcccgac gatcgtcgcc cgctcgccga agcacaggtg
     1441 caccgccgcc gccgccgtcg cgggagcgaa agacgcagcg agcgtcgccg cagtagccgc
     1501 cagcacgatc cgtctcatcc cacgtggtta cccatgatcg cgcctcgcta tcatcccgag
     1561 cggcagcagc aggcgacagg gaaggtggcg aagatgagga tcaaaaagct ctttctcggc
     1621 gcggccctcg cgatgggcgc ggtcggcttc atgccctcgc cggcgcaggc ggtgacgtgc
     1681 tacgacgcgg tcgagccgtt catcgggcgc acctgcacgg attgcggctg ggtgatgatc
     1741 ggaggcaagc cgtacatcct gttctactgc gactgatcca ccgcgacgtc cgaggggctg
     1801 ccggccacgg cggccccttt gcgcgccgtc accagcgcga acgacagcag ctcgccaccg
     1861 tctgcttcga gcatctcgat cacgctcgcg acgtcgtagt ccgattccgc attgtgctgc
     1921 cggcacacct tcgaccacag cagccatttc gcccacgcgt ccggctgcgg gcgcgccgac
     1981 gtcacctcga ccaaccccgt gagggcccag tggtcgcgcc accactccgg ggtgtgccac
     2041 gcgacgcctt cccagccgac gcaacggcga acgtggtccg gaatccgccc gagctcgcgc
     2101 agctcgcgcg tcgtcgacgg aacggctatc ccgacctgcc cgccggactt cacgaagtcc
     2161 agtagatagg ggaggtacgt gtcgtcggtg ccgaagtact cgaacgcgtc gatgctcacg
     2221 accgcgtcga agaacgacgg tgcgaacggg agcttgcgcg cgtccgcgtg caacgcgtgc
     2281 acgcgatctt gaacgcccgc ttctgcgaac gtccgggccg cgtcctccgc cggcacccac
     2341 aggtcgactg cgaagacctc ggcgccgagc tcgcgcgcga ggaagacgga ggtcgcgccc
     2401 aggcccgagc cgaggtcgag cacgcgcatc cccgacgtga tctcgaggtc ccccatcagg
     2461 tcttcgagca gccacagcgg gtgcggcccc atgtcgaggc cgacacccac gcggggtcgt
     2521 agccggacga gcgcgggtac tcggcgggcc ggacgaggtc ttcgaggccg aacatgcacg
     2581 gaactcaacc acagcccccg aatgcgctcg gtccggggat gagagcgccc acgtcctaga
     2641 agggcctgga cctttcggcg agcccggggc ttgttcgagt agaccggcga gatgcgagga
     2701 gccccgctgg actacgaggc cttctaccga agagagttcg acgacgtgtt ccgcggctgc
     2761 atcctcatga ccggttcgag agaggtcgcg ctggatgcga cccaggaggc gttcacccgc
     2821 gcgtacgtgc ggtggcggcg gttgcggcgc catgcgtggg cgggaggatg ggtgatgacg
     2881 accgcgctca acctgtgccg ccgtcagggg cgggtgcccg tcccggtggg gaaggtcgac
     2941 agcagcgcgg cgatcccgga agcaggcccc gatcgcgtcg acctgcagcg cgctctcgct
     3001 acccttccgg cgcggcagcg gcaggcgacg atcctccatt acctcggcga cctccccgta
     3061 ggggcggtgg cggagctcat gcagatcagc gacagcgcgg tgaagtcgca cctccacgcg
     3121 gcacgcggcg cgttgaggcg aacgctcgag gtgaccgatg cctgaggact tgcgcgagct
     3181 cttccgcagg gaggcgtcgg agatcccctc cgcgcagcac gacgcccggg ccgtcacggc
     3241 ggcgggccgg cggcggttgc ggtggcgtcg tgtcgtcacg gcgtccgcgc tgatcgtcgc
     3301 ggtcacggcg ggcgcgggcg tcgtgcggct cgcgagccgt gaagctcacc cgcccgcggc
     3361 gcaggaacgg ctctccgtcc tcgcccgcgt cccgttgtcg tcgtcgtggt cgtcgcactc
     3421 gcgcggcgtc ggtgacctcg cgtacgggct cgggaaggta tgggcactgc aaggacgcga
     3481 gccgacgctc cagcgcatcg acccggggac gaaccgcgtg gaggaggtgc tgacgctggc
     3541 gaacggcgac aacgggaact tctggcacgt cgaggccggc ttcggccggc tatggctgac
     3601 gaacccccat agagacgggc tgctcgtcct cgatccccgg tcgggcgatg tgatcgagac
     3661 agtggaggga gtgccgcgag cgctcgatct cgtcgcgacg aacgagcact tatgggtgag
     3721 cgtcgccacc cgcgacgcgg ggatctaccg agtcgacccg gagacgttcg aggtcgccgg
     3781 gccgatctcg atgggcgccg aatgctgcgc ctccgggatg gtggagcacg gtgggttcct
     3841 ctgggtcgcg cacagtgacg tcggcgacca ggcgcgcaac ccgggggcgc ctcacgacga
     3901 tctggtcttc gacctcacga acgagctcag gaagttcgac gcagagacgc tcgagctcgt
     3961 cgactcgatc cgcctgcccg gcgacacgta ccggccgggc gacacggtcc tgggggggct
     4021 cgtcgccgcg gcgggacacc tgtgggtcgc gcacccggac ggtggcttcg tcgaccgtgt
     4081 cgacccggcg agcgggaccg tcgaatcggt cgacctcaac ggcgtgcgcc cgagcgcgtt
     4141 gacgtatttc cgcggcgcgg tgtgggcgtg gcacatcaac ggttcgaggg tcgtgccgat
     4201 cgatccgtcc gggctggagg tcggcccgac gtcggacctc ggcgaagacg tgagcgggtg
     4261 gcccgtcgag ggcgggggct cgttgtggac cgcagtgcag ccggggaact cgggcgatta
     4321 cctcttgcgc gtcggctctg cggacggacc ggcaccggtc gagacgccgc agcccccgcc
     4381 ggagcagact ccgcctccgc gcggcgaagt cgtggagccc gtgagcgagc gcgagcaagc
     4441 gcagatcttc gttttccgcg cgctcgcaga gacggggttg atgaagccgt tctcggcgcg
     4501 cagctacaac tttgcgtctg ccgaggacac gagccgcgtc ggcgacgcgt ggcggatcgg
     4561 gctcgcggcg agcgactgtg agccgcgagg cgagccgggc agtctctcgt tcacgtgcac
     4621 ggggatctcc ggcgaggacc cgcagtcggg catcccgatg aacgacacgt tcctgacggt
     4681 cgagctgagc gacggtgcgt ggaccgtgac gggggtcgag ggcaacatgc cggaggacga
     4741 tcggtctcgg atcgtgggct acaccgcgga gcagcgcgcg gagccgtcgc attgggagtt
     4801 ccccgcagcc gaggcgtggc cgatgcgatc cggctacatg gtcgagatgt gggcgctatg
     4861 gatagggccg tacccgacgg acgcccgcgg gagcgtgtgc gtggtgacgc tgctggatcg
     4921 ggcgggggag cccctaggcg agccgatccg gtccttcgag gaacccccaa accgagcctt
     4981 cgagacgacg ggcacggccc ggggcctgga cctggagacg atcgacggag acgtagcccg
     5041 ggtggacgtg gactgctacc agcagggctc gtagctcaca gtgcccccgg tgggattcga
     5101 acccacactg tggggttttt aagacccttc tctctgccgg ttggagtacg ggggctccgc
     5161 tatcttgctc gacttaccgt tcgatgacgc ccgag
//
LOCUS       DASQDO010000240         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_513241, whole genome shotgun sequence.
ACCESSION   DASQDO010000240 DASQDO010000000
VERSION     DASQDO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513241"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VEV43_06895"
     CDS             <1..621
                     /locus_tag="VEV43_06895"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HYP23281.1"
                     /translation="SLERAHDKAEAVEAEIEKVAPGADVVVHVEPVAETSGLVERVQA
                     AASRTAGVHEVHNVSVHAFDEGGRRKLHATLHAKVDSGTSVSEAHDLSDRVEAGVRDE
                     LGPDVRVDTHIEPLQPTSFGRDVTAERSDVVDAVRRAAAEEADVVDCHEVLVTQSRDQ
                     LAVVAHVRGRGDLPLRHMHEASERIEKAVHAAHDDVGDVLIHFEPA"
     gene            complement(618..827)
                     /locus_tag="VEV43_06900"
     CDS             complement(618..827)
                     /locus_tag="VEV43_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23282.1"
                     /translation="MTGGILIVLALALVCGRIGLAIGNWWVHSSTSGELESAFPPFIG
                     AVLGAAAGLGIGLGIAYLLGRREQR"
     gene            complement(824..>1578)
                     /locus_tag="VEV43_06905"
     CDS             complement(824..>1578)
                     /locus_tag="VEV43_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013128211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYP23283.1"
                     /translation="RAGLVFELKRQVGTDEIQRREESFIRRAIDSWATNPNIHVLGSH
                     DARRLSIVSFTVKHGKRYLHHNYVVALLNDLFGIQARGGCSCAGPYGHRLLGIDIERS
                     HEFEREIARGCEGIKPGWVRLNFNYFISEAVFEFILEAVHMVANDGWRLLPHYDFEPD
                     TGLWTNQEGVAEPPMSLRSLSYEDGEPVVTLHRAQEPESVLADYLKYAKHIFAEPEVR
                     GPVPGHPRNVSDDFEHLRWFPLPEDVVEPAGS"
BASE COUNT          245 a          538 c          567 g          228 t
ORIGIN      
        1 tccttggagc gcgcgcacga caaagccgag gcggtcgagg cagagatcga gaaggtcgcg
       61 ccgggcgccg acgtcgtcgt gcacgtcgag ccggtcgccg agacgtcggg actggtggag
      121 cgcgtgcagg cggcggcgag ccgtactgcc ggcgtgcacg aggtccacaa cgtgtcggtg
      181 cacgcgttcg acgagggcgg tcgccgcaag ctgcacgcga ccctgcacgc gaaggtcgac
      241 tccggcacgt cggtctcgga ggcccacgac ctgtcggaca gggtcgaagc cggcgtgcgc
      301 gacgagctcg ggccggacgt gcgcgtcgac acgcacatcg agccgctcca gccgacgtcc
      361 ttcggccgcg acgtcacggc ggagcggtcg gacgtggtgg acgcagtgag gcgcgctgcc
      421 gccgaggagg cggacgtcgt ggactgccac gaggtgctcg tgacgcagtc gcgcgaccag
      481 ctcgcagtcg tcgcgcacgt gcgcgggcgc ggcgacctgc ctctgcggca tatgcacgag
      541 gcgtccgagc ggatcgagaa ggcggtccac gcggcacacg acgacgtggg cgacgttctg
      601 atccacttcg agcccgccta gcgctgctcc cgccggccga ggaggtacgc gatcccgagg
      661 ccgatcccga ggcctgcggc ggctcccagc acggccccga tgaacggggg gaacgcgctc
      721 tcgagctcgc cgctcgtgct gctgtgcacc caccagttgc cgatcgccag accgatccgc
      781 ccgcacacga gcgcgagggc gaggacgatc aggatccctc cggtcacgat cccgccggct
      841 ccaccacgtc ttccgggagc gggaaccagc ggaggtgctc gaagtcgtca ctcacgttgc
      901 gggggtggcc cggcaccggc ccccgcacct ccggctcggc gaagatgtgc ttcgcgtact
      961 tcaggtagtc ggcgaggacg gactccggct cctgcgcgcg gtgcagcgtc acgacgggct
     1021 cgccgtcttc gtacgacagc gagcgcaggc tcatcggcgg ctcggcgacg ccctcctggt
     1081 tcgtccacag gccggtgtcc ggctcgaagt cgtagtgcgg cagcagccgc cagccgtcgt
     1141 tcgccaccat gtggacggct tcgaggatga actcgaacac ggcttccgag atgaagtagt
     1201 tgaagttgag ccgcacccac ccgggcttga tcccttcgca cccgcgcgcg atctcgcgct
     1261 cgaactcgtg cgaccgctcg atgtcgatgc cgaggagacg gtgcccgtac ggacccgcgc
     1321 aggagcagcc gccgcgcgcc tgtatcccga agaggtcgtt cagcaacgcg acgacgtagt
     1381 tgtggtggag gtagcgcttg ccgtgcttga cggtgaacga gacgatcgac aatcgccgcg
     1441 cgtcgtggct cccgaggacg tggatgttcg ggttcgtggc ccacgagtcg atggcgcgcc
     1501 ggatgaagga ctcctcgcgc cgttggatct cgtccgtgcc gacctgtcgc ttcagctcga
     1561 agacgagccc ggcccgga
//
LOCUS       DASQDO010000241         4221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_391383, whole genome shotgun sequence.
ACCESSION   DASQDO010000241 DASQDO010000000
VERSION     DASQDO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391383"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..404
                     /gene="ndhC"
                     /locus_tag="VEV43_06910"
     CDS             36..404
                     /gene="ndhC"
                     /locus_tag="VEV43_06910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYP23284.1"
                     /translation="MPDGYFGQYVTIGIFAVVGFVLVFGTLGLARLVRPNVPTPEKYT
                     TYESGIDPIGTGWSQANVRYYVFALLFVIFDVEAVFLFPWAVVFERLGTQAFVEMLIF
                     IAILAFALVYAIKKKVLEWL"
     gene            395..865
                     /locus_tag="VEV43_06915"
                     /pseudo
     CDS             395..865
                     /locus_tag="VEV43_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            960..1541
                     /locus_tag="VEV43_06920"
     CDS             960..1541
                     /locus_tag="VEV43_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYP23285.1"
                     /translation="MPETRHGPSARPQAIGTEEALARVRQALGDRADRIDVNFGQIDL
                     TCKPDQLVQVITALAETPGLECKFLTFVSAIDWSEFETEEGETGRRGLEVLVHVYSPD
                     YVVHCNVHVPVDGEYPVCPSITSIYPGASWHERETYEMFGIVFEGHPRLVNLYLPEDF
                     EGRPLLKSFRLSTRVVKDWPGAKDPEEAAGGGR"
     gene            1545..2705
                     /locus_tag="VEV43_06925"
     CDS             1545..2705
                     /locus_tag="VEV43_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYP23286.1"
                     /translation="MTSTEQDIRQVGQLVEDVGSGTFATEEMILNVGPQHPATHGVFR
                     LLATIDGEVLVDAEPVIGYMHRGYEKLVEVRDYRQITNLVSRMDWLSNIQNELPLALA
                     VERLMEIEVPPRAQYLRVIMCETNRILNHLMFFASFGAELGAITPTFYAFREREDIQL
                     AMEMATGGRLHFGFFRPGGLKEDMPRGFFAQLMKGIRQTIERLPDYENLLIGNEIFKQ
                     RTVGVGVLEKEVAYRYGVSGPIARGSDIHFDVRKDEPYSSYDHFDFDVISAPNGDCFD
                     RFWVLLHEVAESARIIEQAVEALPPGPVMGKVPRVVQAPKGEVYVRCENSLGELGMYL
                     VSDGGGKPYRLKIRTPSFSNVSALPHVLRNTFVADMIAILGSFFFVLGDIDR"
     gene            2702..3718
                     /locus_tag="VEV43_06930"
     CDS             2702..3718
                     /locus_tag="VEV43_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HYP23287.1"
                     /translation="MIEITLTEWQLLVLKLVAILTIVPFAALAGGYAEHKVMAHLQHR
                     LGPMYPGGFHGWATTLADGLKFLYKEDIIPKAADKPVFSLAPAAIFVPVVMIYLVIPM
                     DRTLIVEDLDVGIFYLLAVGSISTIGVLMAGWSSANKYALIGALRAAAQLIAYELPIV
                     LAAASVAMLAGSLSIIQIVEAQDLPFAIWPPGIGLVAFLLFTVGSVAEMTRVPFDMPV
                     AESEIITGPFTEYSGMRYIFAHMFAEMGHMVAFAGIAATLFLGGYKPIVPWAPLEMIP
                     GIVWFLGKTFFMIFLFLWIRPTFPRAREDQLQKFAWKFLIPVGLALILATGAWVLYAP
                     ESIS"
     gene            3851..>4221
                     /locus_tag="VEV43_06935"
     CDS             3851..>4221
                     /locus_tag="VEV43_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HYP23288.1"
                     /translation="MRVTLKHLFKHNTVIEYPDEKQDLPARTRGVIALKEENCTVCML
                     CSRECPDWCIYIDSHKEELPPKREGGRKRKRNVLDRFAIDYALCMYCGICVEVCPYDA
                     LFWSREFEYAEYDIRQLTHEK"
BASE COUNT          680 a         1460 c         1354 g          727 t
ORIGIN      
        1 cgtccggaaa cgagcactag aggagaggct cagccttgcc cgacggctac ttcggtcagt
       61 acgtcaccat cgggatcttc gccgtcgtcg gcttcgttct ggtcttcggg acgctgggac
      121 tcgcgaggct cgtccggccc aacgtcccga cccccgagaa gtacacgacc tacgagtccg
      181 gcatcgaccc gatcggcacg gggtggagcc aggcgaacgt ccgctactac gtcttcgcgc
      241 tcctcttcgt gatcttcgac gtcgaggcgg tctttctgtt cccgtgggcc gtcgtgttcg
      301 agcggctcgg cacccaggcc ttcgtcgaga tgctgatctt catcgcgatc ctggccttcg
      361 ccctcgtcta cgccatcaag aagaaggtgc tcgaatggct gtgatcgaga aggtccccct
      421 cccgggaccg gtctcctggc tcctcaactg gagccggcgc tactcgctgt ggatgctcca
      481 gttcggcctc gcctgctgcg ggatcgagat gggcgccgct atcgccagca agttcgacat
      541 catccggttc ggcgtgatcc cgttcccggc gtcgccccgc caggccgacc tcatggtcgt
      601 cgccggcacg gtgacggaca agatggcggc cccgatcaag cggatctacg accagatgcc
      661 cgagcccaag tacgtgatct cgatgggctc gtgcgccaac tgcgggggcc cgtactggga
      721 ctcgtactcg gtcacgaagg gcgtcgacca gatcatcccc gtcgacgtct acgtgccggg
      781 atgcccgccg cggcccgagg ccctgctgca gggcgtcatc cggctccagc agctgatcgc
      841 ccaggaggac gtccgcgaga agtggaaggg ccacacgcag gagatcccgc acatccccga
      901 gcccggcctg gcgggtggga tctaggttgg cacggcccga cgtcggccgc ggggcggcca
      961 tgcccgagac gcgacacggg ccctccgccc ggccgcaggc catcggtacc gaggaggcgc
     1021 tagcgcgcgt ccgtcaggct ctcggcgacc gagcggatcg catcgacgtc aacttcggcc
     1081 agatcgacct gacctgcaag cccgaccagc tcgtacaggt gattaccgcg ctggccgaga
     1141 cgcccgggct ggagtgcaag ttcctcacgt tcgtctcggc gatcgactgg tccgagttcg
     1201 agaccgagga aggcgagacg ggccggcgcg gcctcgaggt cctcgtccac gtctactcgc
     1261 ccgactacgt cgtccactgc aacgtccacg tcccggtcga cggcgagtac ccggtgtgcc
     1321 cgtcgatcac gtcgatctat cccggcgcga gctggcacga gcgcgagacc tacgagatgt
     1381 tcggcatcgt cttcgaaggc catccgcggc tcgtgaacct ctaccttccc gaggacttcg
     1441 agggccggcc cctgctgaag tcgttccgcc tcagcacccg cgtcgtgaag gactggccgg
     1501 gcgcgaagga ccccgaggaa gccgcgggcg gtgggaggta ggcgatgacg tcgaccgagc
     1561 aggacatccg ccaggtgggg cagctcgtcg aggacgtcgg cagcggcacg ttcgcgaccg
     1621 aggagatgat cctcaacgtc gggccgcagc accccgcgac gcacggcgtc ttccgcttgc
     1681 tcgcgacgat cgacggtgag gtgctcgtcg acgccgagcc cgtcatcggt tacatgcacc
     1741 gcggctacga gaagttggtc gaggtccgcg actaccgcca gatcacgaac ctcgtgtcgc
     1801 ggatggactg gctctcgaac atccagaacg agctgccgct cgcgctcgcc gtcgagcgcc
     1861 tgatggagat cgaggtcccg ccccgcgccc agtacctgcg cgtgatcatg tgcgagacca
     1921 accggatcct gaaccacctg atgttcttcg cgtcgttcgg agccgagctc ggcgcgatca
     1981 cgccgacgtt ctacgcgttc cgcgagcgcg aggacatcca gctcgccatg gagatggcga
     2041 ccggtggccg gctccacttc gggttcttcc gtccgggcgg cctcaaggag gacatgcccc
     2101 gcgggttctt cgcgcagctc atgaagggca tccgccagac gatcgagcgc ctgcccgact
     2161 acgagaacct cttgatcggc aacgagatct tcaagcagcg cacggtcggc gtcggcgtcc
     2221 tcgagaagga ggtcgcgtac cgctacggcg tgtccggccc gatcgcccgc ggctccgaca
     2281 tccacttcga cgtccgcaag gacgagccgt actcgagcta cgaccacttc gacttcgacg
     2341 tgatctcggc ccccaacggc gactgcttcg accgcttctg ggtcctgctc cacgaggtgg
     2401 ccgagtccgc gcggatcatc gagcaggccg tcgaggcgtt gccgcccggc cctgtcatgg
     2461 gcaaggtccc ccgtgtggtc caggccccca agggcgaggt ctacgtgcgc tgcgagaact
     2521 cgctcggcga gctcgggatg tacctcgtgt ccgacggcgg gggcaagccg taccggctca
     2581 agatccgcac gccgtcgttc tcgaacgtct cggcgctgcc gcacgtgctc cgcaacacgt
     2641 tcgtcgccga catgatcgcc atcctcgggt cgttcttctt cgtgctgggg gacatcgacc
     2701 ggtgatcgag atcacgctga ccgagtggca gctcctcgtc ctcaagctgg tggcgatcct
     2761 cacgatcgtt ccgttcgccg cgctggccgg ggggtatgcg gagcacaagg tgatggcgca
     2821 cctccagcac cgcctcggcc cgatgtaccc gggcgggttc cacggctggg cgaccacgct
     2881 cgcggacggg ctgaagttcc tctacaagga ggacatcatc ccgaaggccg ccgacaagcc
     2941 ggtcttctcg ctagcgccgg ccgcgatctt cgtgccggtc gtgatgatct acctcgtcat
     3001 cccgatggac cggacgctga tcgtcgaaga cctcgacgtc ggcatcttct acctcctcgc
     3061 cgtcggctcg atctcgacca tcggcgtgct catggcgggc tggtcgtcgg cgaacaagta
     3121 cgcgctgatc ggcgcgctgc gcgccgcagc ccagctgatc gcgtacgagc tcccgatcgt
     3181 gctcgcggcc gcctcggtcg cgatgctcgc gggatcgctc tcgatcatcc agatcgtcga
     3241 ggcccaggac ctgccgttcg cgatctggcc gccgggcatc gggctcgtcg cgttcctgct
     3301 gttcacggtc gggtcggtcg cggagatgac gcgcgtgccg ttcgacatgc cggtcgcgga
     3361 gtccgagatc atcacgggac cgttcaccga gtactcgggg atgcgctaca tcttcgccca
     3421 catgttcgcc gagatggggc acatggtcgc gtttgccggc atcgccgcga cgctgttcct
     3481 cggcggctac aagccgatcg tcccgtgggc gccgctcgag atgatcccgg ggatcgtgtg
     3541 gttcctcggc aagacgttct tcatgatctt cctgttcctg tggatccgcc cgacgttccc
     3601 gcgggcacgt gaggaccagc tccagaagtt cgcctggaag ttcctcatcc ccgtgggcct
     3661 cgccctgatc ctcgcgaccg gcgcatgggt cctctacgcc cccgagtcga tcagctagga
     3721 gaccttgatg gcgatcgacg agcaccagac gacgacgacg agcaactccg tccaccacga
     3781 ccacccgtcc tcgatcggga acctgaagcg ggcgcgcaag ccgggcttcg gcctcggcct
     3841 cctcaaggga atgcgcgtca cgctcaagca cctcttcaag cacaacaccg tcatcgagta
     3901 cccggacgag aagcaggacc tcccggcgcg cacgcgcggc gtgatcgcgc tgaaggagga
     3961 gaactgcacg gtctgcatgc tgtgctcgcg cgagtgcccc gactggtgca tctacatcga
     4021 ctcgcacaaa gaggagctcc cgccgaagcg tgaggggggc cgcaagcgca agcgcaacgt
     4081 cctcgaccgg ttcgcgatcg actacgcgct ctgcatgtac tgcggaatct gcgtcgaggt
     4141 gtgcccctac gacgcgctgt tctggagccg cgagttcgag tacgccgagt acgacatccg
     4201 ccagctcacg cacgagaagg a
//
LOCUS       DASQDO010000242         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1064708, whole genome shotgun sequence.
ACCESSION   DASQDO010000242 DASQDO010000000
VERSION     DASQDO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064708"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VEV43_06940"
     CDS             complement(<1..723)
                     /locus_tag="VEV43_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HYP23289.1"
                     /translation="MSRRIPYVQQLEISDCGAACLAMSLAFHGKHVSPEEVRNATGTG
                     RDGVDAASIVRAARAYDMRARGVKVEVDGVKFLPKGAILHWEFNHFVVFERVTRAGVV
                     VVDPALGRQRISMEKFGQSFTGVALIIEPDEGFSKDRRRAESAWRYVRPMMKQSGLLG
                     RVGVVSLFIQLLALAVPIMTGVLVDRIVPAGDRDLLFVLVTGLLGMALFHMFASFVRA
                     HLLLNVRTQLDLRISMGFIHHLV"
     gene            complement(720..1241)
                     /locus_tag="VEV43_06945"
     CDS             complement(720..1241)
                     /locus_tag="VEV43_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23290.1"
                     /translation="MPEREPLFREEAVEHQRKKASAADLVRLSAGWAKLGFWLVLGAV
                     VVVVATASIARVERVALAVAMAEDDGRVTGLMPAGQGAAPVQGSTATYVPVDGGGEQD
                     VRVVEIEEDMPADEAREEFPSLAFAIAGPVTVVRAEGFEGGFAYGQLRVKTGSEPVIV
                     SFVPGLRELFGES"
     gene            complement(1253..>1603)
                     /locus_tag="VEV43_06950"
     CDS             complement(1253..>1603)
                     /locus_tag="VEV43_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HYP23291.1"
                     /translation="AADEARSLAELWQSVGVICPEALVGAAPAALAGAGVDFGTWEEG
                     LLEKAVILLPPLAAKGLEFDAVVVVEPALLMLEDKGPRRLYVALTRAVQQLTIVHDAP
                     LPDVLQGASSEPVP"
BASE COUNT          271 a          593 c          538 g          201 t
ORIGIN      
        1 cacgaggtgg tggatgaacc ccatcgagat cctgaggtcg agctgggtcc ggacgttcag
       61 caacaggtgc gcgcggacga acgacgcgaa catatggaac agcgccatcc cgagcaggcc
      121 ggtgacgagc acgaacaaga ggtccctgtc gcccgccggg acgatccggt cgacgagcac
      181 cccggtcatg atcggaacgg cgagcgcgag cagctggatg aacagcgaca cgacgccgac
      241 tcgcccgagc agccccgact gcttcatcat cggacgcacg tagcgccacg cgctctcggc
      301 gcggcggcga tccttgctga agccctcgtc cggctcgatg atcagcgcga cgcccgtgaa
      361 tgactggccg aacttctcca tcgagatccg ctggcggccg agcgcagggt cgacgaccac
      421 gacgcctgcg cgggtgacgc gctcgaagac gacgaagtgg ttgaactccc agtggaggat
      481 cgcgcccttg ggcaggaact tgactccgtc gacctcgacc ttcacgccgc gggcgcgcat
      541 gtcgtaggca cgggcagcgc gcacgatcga ggcagcgtcg acaccgtcgc gcccggtgcc
      601 ggtcgcgttc cgcacctcct cgggtgagac gtgcttgccg tggaacgcaa gggacatcgc
      661 gaggcacgcg gccccgcaat cggagatctc gagctgctgc acgtacggga tacgccggct
      721 cacgactctc cgaacagctc ccgcagcccc ggcacgaacg agacgatcac gggctcggac
      781 cccgtcttga cgcgcagctg cccgtacgcg aagccgccct cgaagccctc ggcgcgcacg
      841 acggtgaccg gccctgcgat agcgaaggcg aggctgggga actcctcgcg tgcctcgtcg
      901 gccggcatgt cctcttcgat ctcgacgacg cgcacgtcct gctcgccgcc cccgtcgacg
      961 gggacgtagg tcgcagtcga cccctgcacc ggcgcggccc cctgaccggc gggcatgagc
     1021 ccggtgacac gtccgtcgtc ctcggccatc gcgacggcca gagcgacgcg ctcgacgcgg
     1081 gcgatcgaag ccgtggcgac taccacgacg accgcgccca ggacgagcca gaagcccagc
     1141 ttcgcccacc ccgccgacag ccgcacgagg tcggccgccg acgccttctt cctctggtgc
     1201 tcgaccgcct cctcgcggaa cagcggctca cgctccggca tcgcccgcca tgctacggga
     1261 ccggctcgga cgaagcgccc tggagcacgt ccgggagcgg cgcgtcgtgg acgatcgtga
     1321 gctgctggac cgcgcgcgtg agcgcgacgt agagccggcg cggccccttg tcctcgagca
     1381 tcagcagcgc gggctcgacc accacgaccg cgtcgaactc gaggcccttc gcggcgagcg
     1441 gcggcagcag gatcacggcc ttctcgagca acccctcctc ccaggtaccg aagtcgacgc
     1501 cggcaccggc gagcgcggcg ggggccgcgc cgacgagggc ctcgggacag atgacgccga
     1561 cgctctgcca cagctccgcc agggaacgcg cctcgtctgc ggc
//
LOCUS       DASQDO010000243         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_819743, whole genome shotgun sequence.
ACCESSION   DASQDO010000243 DASQDO010000000
VERSION     DASQDO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819743"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VEV43_06955"
     CDS             <1..787
                     /locus_tag="VEV43_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP23292.1"
                     /translation="LPLLVANGIGANLAVWRRTLYELAAEREIVTWDHRGLFESKPPA
                     SDRLDAAAHAEDAIAAADHAGLDRFALVSWSSGGPIALQVANRHPERIAALVLVNAGF
                     GHPPARLLRHLELTSALPMLAGVAKHFSARLQGPFRSFVSRPTIAGLVRQSGLVGPTA
                     DIPAMVDFLQGMASCDLRSLLEAYEEVAGDNSASLLGKVQAPVLLVAGSKDPFTPAST
                     MEEMERAIPDARLEVYEKATHYLPMEHPARLREDLKQFVDSRA"
     gene            complement(784..1872)
                     /locus_tag="VEV43_06960"
     CDS             complement(784..1872)
                     /locus_tag="VEV43_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYP23293.1"
                     /translation="MNRSNESRPGEVPRLFALTASYSWRFLVVLAAVCVFVYALVTLR
                     LIFIPVVVALLISTLLVPLAERLRKRGLPSLLATWIVIAGALGLVAGIVSLIAPAIAG
                     ELDNLGRDVRKGTEDVITWLTEGPLDLTRAQIDGYVDQLSEQLSAQRSSIVSGAFKGA
                     YLLVEVVVGALLTLVLTFFFVKDGNRLATAILGLFPERRHDDIRTVSKRSWDALGAYI
                     RGTAIVGLVDAVAIGGTMLVLGVPLVGPIAVITFFGAFFPLVGAVVAGVIAALVALVT
                     TGFLPALILAAATIVVQQVEGDVLQPLVLGRAVHLHPLVILLSLTTGAIVAGIAGAFL
                     AVPVAAVAAVVVAHLRGRDPGTVAEAED"
     gene            complement(1910..2428)
                     /locus_tag="VEV43_06965"
     CDS             complement(1910..2428)
                     /locus_tag="VEV43_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23294.1"
                     /translation="MTRRYALLLTAAATAAVALLVVAVWPGPPAHTLPRRDPDRIYRM
                     LLDSDFSDLGLQHGLEFTGVRGMLPPDEAERERHWLLYAESDGPGGRHRIAFYIPLGE
                     RLEEMEEHSRRSRRCAPYFDVGWECLGSIRGTYVRGDVVCLRPRCEAHKAQAETLLYL
                     GMRHLQRVLAST"
BASE COUNT          441 a          904 c          987 g          349 t
ORIGIN      
        1 actgccgctg ctggtcgcga acgggatcgg ggcgaacctc gcggtgtggc ggaggacgct
       61 ctacgagctg gcggcggagc gcgagatcgt gacgtgggac caccgcgggc tgttcgaatc
      121 gaagccgccc gcgtcggatc gcctcgacgc ggccgcgcac gcggaggacg cgatcgcggc
      181 ggccgaccac gcgggactcg accggttcgc gctggtgtcg tggagctctg gggggccgat
      241 cgcgctccag gtcgcgaacc gccacccgga acggatcgct gcgctcgtgc tcgtcaacgc
      301 ggggttcggg catcctccgg ctcggctgct gagacacctc gagctgacgt ccgcgctgcc
      361 gatgctcgcc ggcgtcgcga agcacttctc ggcgcggttg caggggccgt tccggtcgtt
      421 cgtgtcgcgg cccacgattg ccgggctcgt gcggcagtcg ggcctcgtcg ggcccaccgc
      481 ggacatcccc gcgatggtcg acttccttca gggcatggcg tcgtgcgacc tgcggagcct
      541 gctggaggcg tacgaggagg tcgcgggcga caacagcgcg agcctgctcg ggaaggtgca
      601 ggcgccggtg ctgctggtcg ccgggagcaa ggacccgttc acgccggcct cgacgatgga
      661 ggagatggag cgggcgatcc ccgacgcgcg cctggaggtg tacgagaagg cgacgcacta
      721 cctgccgatg gagcacccgg cgagactgcg ggaagacctg aagcagttcg tggactcgcg
      781 ggcctagtcc tcggcctcgg ccaccgtgcc ggggtcccgg cccctcaggt gcgcgaccac
      841 gactgccgcg accgcggcta ccggcacggc caggaacgct cctgctatgc cggcgactat
      901 cgctcccgtc gtcagcgaca gcaggatcac gagcggatgc aggtggacgg cgcggccgag
      961 caccagcggc tggagtacgt cgccctcgac ctgctgcacg acgatcgtcg ccgccgcgag
     1021 gatcagcgcg gggaggaagc cggtcgtgac gagggcgacg agggccgcga tcactccggc
     1081 gacgactgcc cccacgagcg ggaagaacgc accgaagaac gtgatgacgg cgatcgggcc
     1141 caccagcggg acgccgagca cgagcatcgt ccccccgatc gcgacggcgt cgacgaggcc
     1201 gacgatcgcg gtgcccctga tgtaggcgcc tagtgcgtcc cacgacctct tcgacacggt
     1261 ccggatgtcg tcgtgacgtc gctcggggaa caacccgagg atcgcggtgg cgaggcggtt
     1321 gccgtccttc acgaagaaga acgtgagcac gagggtcagc agcgcgccta cgaccacctc
     1381 gaccaggaga tacgccccct tgaaggcgcc gctgacgatc gagctgcgct gcgcgctcag
     1441 ctgctcggag agctggtcga cgtagccgtc gatctgcgcc ctcgtcaggt cgagcggccc
     1501 ttcggtcagc cacgtgatga cgtcctcggt gcccttgcgc acgtcccggc cgaggttgtc
     1561 gagctcgccc gcgatcgccg gagctatcag ggacacgatc ccggcgacca gcccgagtgc
     1621 ccccgctatg acgatccacg tcgcgagcag cgagggaagc cccctctttc gcagccgctc
     1681 cgcgagcggg accagcagcg tcgagatgag cagcgcgacg accacgggga tgaagatcag
     1741 gcgcagcgtc acgagggcgt agacgaagac gcataccgcg gcgaggacga cgaggaaccg
     1801 ccagctgtag gacgcggtca gggcgaacag ccgtgggacc tcgccggggc gggactcgtt
     1861 cgaccggttc acgggctgca tcttgcccga gagccgccgc ggcgaaccac tacgtggacg
     1921 cgagcacccg ctggagatgc ctcatgccga gatacaggag ggtctcggcc tgggccttgt
     1981 gggcctcgca gcgcggtcgc aagcagacga cgtcgcccct tacgtaggtc ccgcggatcg
     2041 agcccaggca ctcccaaccg acgtcgaagt acggggcgca ccgccgggac ctgcgcgaat
     2101 gctcctccat ctcttcgagc cgttcgccga gcgggatgta gaacgcgatc cggtggcggc
     2161 cgccggggcc gtcggactcc gcgtagagga gccagtggcg ttcgcgctcg gcctcgtccg
     2221 gcgggagcat cccgcgcacg ccggtgaact cgaggccgtg ctggaggccg aggtccgaga
     2281 agtcgctgtc gagcagcatc cggtagatgc ggtcggggtc gcgccggggc aaggtgtgtg
     2341 caggggggcc gggccacact gcgacgacga gaagtgcgac cgccgcagtc gcggccgccg
     2401 tcaggaggag tgcgtaccgc cgcgtcattg ctcgatccat cggcgcgtcg ggctcgcgag
     2461 gagcagcatc agggcgaagg cgccggccgc gacgccggcc cacgcggtgg gctcgtcgaa
     2521 ggccgcggtc acgccggcga cgatccctcc cgcagaggcg atcacgaaca gcaaccagat
     2581 cgcgcgctca cggacccaga gctaccacag gagcgcgagg aacacgagcg tgaagacgat
     2641 cgccgggaag ccgaacgtgc tgggatcgcg gatggccgag a
//
LOCUS       DASQDO010000244         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_330344, whole genome shotgun sequence.
ACCESSION   DASQDO010000244 DASQDO010000000
VERSION     DASQDO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330344"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VEV43_06970"
     CDS             complement(<1..696)
                     /locus_tag="VEV43_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYP23295.1"
                     /translation="MKDVRARVPAEIAPSLKKRRQPEWVEPMFATLTKDYFSDPAWVF
                     EPKLDGIRCIAYKRGSSVKLLSRNRLELTPTYPDVADALASEKPDFVVDGEVAAVKGE
                     ITSFSLLQQVHRQQVPVVYFCFDLLHLDGYDLTGLPLTARKDLLRESLAFDDPLRYVS
                     HVEEEGEAYYREACKRGWEGLIAKRADGRYARGRSKDWLKFKCSFEQELVIGGYTNPE
                     GARSHFGALLLGYY"
     gene            complement(719..1087)
                     /locus_tag="VEV43_06975"
     CDS             complement(719..1087)
                     /locus_tag="VEV43_06975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYP23296.1"
                     /translation="MAFRELLGIEIEERGDGRAKLVFRASDDHLNDGGIVHGGAIATL
                     ADCAMGSALASTLEEGEQPVTVEAKVNYLEPGERGTIAAEATVRRKGNRFTVLEAEVV
                     QQESGEHIAYATATFTILQT"
     gene            1191..3290
                     /locus_tag="VEV43_06980"
     CDS             1191..3290
                     /locus_tag="VEV43_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYP23297.1"
                     /translation="MERLHDILGYVQLVLYVGLGLIAFMQWRKRGGKPAAWVAATFAA
                     LASVVVISQFLPEDDVSGTVDVVQRFVIAILVLFPYFLFRFMASFESPGKVLHVVAAL
                     LTGATVVATFLLDHFPEPNEQRTDAFQAYIYLLLGQWVVLLGAVTFRLWHAGRGQPVV
                     ARRRMRTMSIGSAGLAITLVIAGTASASEDVTGVQIATSLLALATGPFFLLGFAPPAF
                     VRNAWRQQGSSRLQEAEFALMEALSPSDITDVLLPEVTELAAGRGSLVIDRGGLITGV
                     HGLTAPEAARLASQFKNGGADGVTRDAGTITVPMRSGKLVVVTSPYTPFFGQEEMDEL
                     RRLALLADLALARTEHSRRERELTDQLLEAQRLARIGSWEWDIPIDRVTWSDQMYRML
                     GYEPGEVEATGELYNEHVHPDDREVVAAKSQETANGNTAMDYEHRMVRRDGTPLVIHV
                     RGRISATEDGQPIRMIGTVQDVTEQREQEALRERFIANAAHELRTPLTSLLGFVEMLS
                     RARHRLDEAKLQTMFDAMTRSGDRLAVLIENLLDLSKLQAGQVQVDIEPVSIDTLAHE
                     VVASTPPPDGRRVEVEVTDTPVALADKHRLDQVVTNLLTNAYRYGGPNISLSGARRNG
                     DVILSVTDDGPGVEDQLVGELFQPFSRGSTSSTIGGSGLGLAIVKMLVEACGGEIWHE
                     ALQPTGARFCVKLKSAE"
     gene            3287..>3574
                     /locus_tag="VEV43_06985"
     CDS             3287..>3574
                     /locus_tag="VEV43_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP23298.1"
                     /translation="MTGKILVVDDEPDLRLLVRMTLEGNGYEVEEASTAAEAITQALV
                     VRPRLILLDIRLPDAEGFEVLKTLKRTPELEQTPVVMMSAHSSPPTLRKAEV"
BASE COUNT          543 a         1227 c         1233 g          571 t
ORIGIN      
        1 gtagtagccg aggagcagcg cgccgaagtg cgaccgcgcc ccctcgggat tggtgtagcc
       61 gccgatcacg agctcttgct cgaaggagca cttgaacttg agccagtcct tcgaccggcc
      121 gcgggcgtag cggccgtcgg cgcgcttggc gatcagcccc tcccagccgc gcttgcaggc
      181 ttcgcggtag tacgcctcgc cctcctcctc gacgtggctc acgtaacgca gcgggtcgtc
      241 gaacgcgagc gactcacgca agaggtcctt gcgcgccgtc agcgggagcc cggtgaggtc
      301 gtagccgtcg aggtggagca ggtcgaagca gaagtacacg acgggcacct gctggcggtg
      361 cacctgctgg agcagcgaga agctcgtgat ctcgcccttt accgccgcga cctcgccgtc
      421 gacgacgaag tcgggcttct ccgacgcgag agcgtccgcg acgtcggggt acgtcggcgt
      481 gagttcgagc cggttgcgcg acaggagctt caccgaggag ccgcgcttgt acgcgatgca
      541 cctgatgccg tcgagcttcg gctcgaagac ccacgcgggg tccgagaaat agtccttcgt
      601 cagcgtcgcg aacatgggct cgacccactc gggctgacgt cgtttcttca gcgagggcgc
      661 gatctcggcg gggacgcgcg cgcgtacgtc tttcacggcg ccaaggtacc cgccgcgctc
      721 aggtctgcag gatcgtgaac gtcgccgtcg cgtaggcgat gtgctcgccc gactcctgct
      781 ggacgacctc ggcttcgagg accgtgaacc ggttcccctt gcgccggacg gtcgcctcgg
      841 ccgcgatcgt cccgcgctct ccgggctcga ggtagttcac cttcgcctcg acggtcacgg
      901 gctgctcgcc ttcttccaga gtggacgcaa gcgcggaccc catcgcgcag tcggccagcg
      961 tcgcgatcgc gccgccgtgg acgatgcccc cgtcgttgag gtggtcgtcg ctcgcccgaa
     1021 atacgagctt tgcccgcccg tcgccgcgct cttcgatctc tatcccgagc aactccctga
     1081 aggccatgcc ggtcttcttg cccgtggcgc ggcggacgaa tcctcccgcg ccctccaatt
     1141 ggaacctaga attggacggc ccctggcttc gccgcgatcg gagagctctt ttggagaggc
     1201 ttcacgacat cctcggctac gtacagctcg tcctgtacgt ggggctggga ctgatcgcct
     1261 tcatgcagtg gcgcaagcgc gggggcaagc cggcggcttg ggtggccgct acgttcgccg
     1321 cgctggcaag cgtcgtcgtc atcagccagt tccttcccga ggacgacgtc agcggcacgg
     1381 tcgacgtcgt ccagcgtttc gtgatcgcga tcctcgtcct gttcccctac ttcctgttcc
     1441 ggttcatggc gtcgttcgag tcgccgggga aggtgctgca cgtcgtcgcg gcgttgctga
     1501 ccggcgcgac cgtcgtcgcg acgttcctcc tcgaccactt ccccgagccg aacgaacagc
     1561 gcacggacgc gttccaggct tacatctact tgctgctcgg ccagtgggtc gtcctgctgg
     1621 gcgccgtgac gttccgcctg tggcacgcgg gacgcggcca gccggtggtc gcccggcggc
     1681 gcatgcggac gatgagcatc gggtcggccg gcctggccat cacgctcgtg atcgccggga
     1741 cggcgtcggc gtccgaagac gtcaccggcg tccagatcgc gacctcgctg ctcgcgcttg
     1801 cgaccgggcc gttcttcctg cttgggttcg cgcctccggc gttcgtacgc aacgcgtggc
     1861 gccagcaggg gagcagccgt ctgcaggagg cggagttcgc gctcatggag gcgctgtcgc
     1921 cgtcggacat caccgacgtc ctgctgcccg aggtgacgga gctggcggcg ggacgagggt
     1981 cgctcgtgat cgaccgcggc ggcttgatca ccggcgtcca cggcctgacg gcccccgagg
     2041 cggcccggct cgcgtcccag ttcaagaacg ggggcgccga cggcgtcacg cgcgatgccg
     2101 gcacgatcac ggtcccgatg cggtcgggga agctcgtcgt cgtcacgagc ccctacacgc
     2161 cgttcttcgg acaggaggag atggacgagc tgcggaggct cgcgctcctc gccgacctcg
     2221 cgctcgcgcg caccgagcat tcccggcggg agcgtgagct gacggaccag ctcctcgagg
     2281 cgcagaggct ggcgcgcatc ggcagctggg agtgggacat acccatcgac cgcgtcacct
     2341 ggtcggacca gatgtaccgc atgctcggat acgagcccgg cgaggtcgag gcgaccggcg
     2401 agctctacaa cgagcacgtc catccggacg accgcgaggt cgtcgccgct aagtcgcagg
     2461 agacggcgaa cggcaacacg gctatggact acgagcaccg aatggtccgc cgcgacggga
     2521 cgcccctcgt catccacgtg cgcggccgga tcagcgccac ggaggacggg cagcccatcc
     2581 gcatgatcgg gaccgtccag gacgtcaccg aacagcgcga gcaggaagcg ctgcgcgagc
     2641 ggttcatcgc gaacgccgcg cacgagctcc gcaccccgct tacgtcgctg ctcggcttcg
     2701 tcgagatgct gtcgcgggcg cggcaccgcc tcgacgaggc caagctccag acgatgttcg
     2761 acgcgatgac gcgttcgggc gaccggctcg cggtgctcat cgagaacctg ctcgacctgt
     2821 cgaagctcca ggcgggccag gtgcaggtcg acatcgagcc ggtgagcatc gacacgctcg
     2881 cgcacgaggt cgtggcgagc acaccgccgc ccgacggaag gcgcgtcgag gtcgaggtga
     2941 ccgacacgcc ggtagccctc gcggacaagc accgcctgga ccaggtcgtg acgaacctgc
     3001 tcaccaacgc ctaccggtac ggggggccga acatctccct gagcggggcg cgccgcaacg
     3061 gcgacgtgat cctgtcggtg acggacgacg gcccgggcgt cgaggaccag ctcgtagggg
     3121 agctgttcca gccgttctcg cgcggctcca cgtcgtcgac gatcggcggc tccgggctcg
     3181 ggctggcgat cgtgaagatg ctcgtcgagg cctgcggggg cgagatctgg cacgaggctc
     3241 tccagcccac cggcgcccgc ttctgcgtga agctcaagag cgcggagtga cgggcaagat
     3301 cctcgtcgtc gacgacgagc ccgatctccg cttgctcgtg cggatgacgc tcgagggcaa
     3361 cggctacgag gtcgaggagg cgtcgacggc ggccgaggcg atcacgcaag ccctcgtggt
     3421 caggccgcgg ctgatcctgc tcgacatcag gctgccggat gccgaggggt tcgaggtcct
     3481 gaagaccttg aagaggactc cggagctgga gcagaccccc gtcgtgatga tgtccgccca
     3541 ctcgagcccg ccgaccctgc gcaaggccga ggtc
//
LOCUS       DASQDO010000245        10886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_820142, whole genome shotgun sequence.
ACCESSION   DASQDO010000245 DASQDO010000000
VERSION     DASQDO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820142"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VEV43_06990"
     CDS             complement(<1..318)
                     /locus_tag="VEV43_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYP23299.1"
                     /translation="MPASRRVGDATRSVHGGERRPGPEGSIVFPIYQSTVYATKPGTD
                     DPDDIDYIRLSNTPSQAYLHDKLAALEGAEAAVATASGMSAMTTILATYLQSGDHMIA
                     AEGL"
     gene            complement(412..621)
                     /locus_tag="VEV43_06995"
     CDS             complement(412..621)
                     /locus_tag="VEV43_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23300.1"
                     /translation="MRPFRKRQERVGILARRHHRGQSHDRDERALAGPLFVMYELAIL
                     ALRLVREGPREECRRAGRACVRGTA"
     gene            complement(747..932)
                     /locus_tag="VEV43_07000"
     CDS             complement(747..932)
                     /locus_tag="VEV43_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23301.1"
                     /translation="MTRTRLARTIAAVALVLGAGSVVAPAAQAAPECKGATAVIQCWD
                     QRVMECLRNLSLPPGRC"
     gene            1126..2406
                     /locus_tag="VEV43_07005"
     CDS             1126..2406
                     /locus_tag="VEV43_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23302.1"
                     /translation="MASVVVLAALAVSLVAQDRARGWHTEAWVAPLLDRDGYTLNVVR
                     GDGYLAWTEAGICCASAMQYANGAGIGPEHARTFKVMLRTPGGKTVQLSRDGVWAMAG
                     GIDRGVLAYATGKPRADIRLLDLKTRKHRRLPRGVNTNLVEYHPTLSGKWLLFGRIRP
                     SPRPNDAIRNRTSVILTNLETGRSRVLAEGKNYRSYTYPGQVNGDWLTYTACKFNKYG
                     PNCGVTRYRISSGKRASADLDGFCCKHGDPHTENHMSAPGSVRASAVTDDGLVYMAMT
                     SGQNTSWCGYTEVWAWDREARLAWMFGEYEGRSADNPYGGLVVTSLYVDGSRVLFDGY
                     TTWMGEDPVHHYPTCYVETSALFVMGHGSPPPSAATPPPTPTPSPSATEPAETPTPTP
                     TPTPEPTPTPTPTPTPSPSPSPSPAPSCPLPVCV"
     gene            2425..2901
                     /locus_tag="VEV43_07010"
     CDS             2425..2901
                     /locus_tag="VEV43_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HYP23303.1"
                     /translation="MLENVVVGIVMVVGLAGVVVPGLPGTLLILAAGVGWAFFRDGGA
                     GPWVVVGFMAALFVLGTILKYVLPGRRLSGELPRSTIFSGVVGAIVGFVVLPPLGLLV
                     GGVVGVYLAETRRVGPGAEARRSTVAVLKAIGVGILAELTAGVLMIVTWLVGLAAT"
     gene            complement(2898..4319)
                     /locus_tag="VEV43_07015"
     CDS             complement(2898..4319)
                     /locus_tag="VEV43_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HYP23304.1"
                     /translation="MRAGRGPLVIGLVAIAAATLAGGVPAGGVGAAQEDPATRSVMVV
                     SNNWAGTATIVDARSREVLTTINVVPDKDEELQHVNTPSPSHPAGPAFYVAIQQGVGE
                     GNDQYVDDSFTTIDGKYLAVSRPSLRDVVWIDLAKAAAAGEAGDPASIVAEAEMDGYR
                     TDHMGVSPDGRRLVVSDSTARQVVEFSMVDETLPDGTEVTMGERLRSFESGDTPHENN
                     YSADGRRIFHASIGRVYTPGDEGDSDSSGGDTSKGDRWLQVVDNDSFEVKRRWDMGQE
                     LAEAGHPDMSSAVRPVALTPDEKIMYAQVSFFHGFVEFHLNKKDETGGGDYELGGLAE
                     PKTGVVTRIVDLPIAEDVKDMPREQYVLDSAHHGIAMNARGNKLCVAGTMSDYAASVD
                     RKTFTPKVFDGAARFVEDVEYSKPYWAVEGPGNTCWMSMSGSDLVTIISFGKEKVVAE
                     VPVGDHPQRVRHGNILESIVAGF"
     gene            4458..5048
                     /locus_tag="VEV43_07020"
     CDS             4458..5048
                     /locus_tag="VEV43_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HYP23305.1"
                     /translation="MPTIEERRFQDRELTNEEVQGSEFRRCRFSGVVFSDDEYTDATF
                     EHCKFLSCDFSSTRHRNASFRNCDFAGSSLASTRFDTCRLMGSDLSEARLWNVECDGG
                     NWSYVNLRMQELAGWRAPGIVLRGADLYQADLTGADLTGADLTEANLDKVKLKDADLR
                     GANLTRTNLAELVLDKTRLDFEQCVLFAVAHGARVD"
     gene            complement(5052..5213)
                     /locus_tag="VEV43_07025"
     CDS             complement(5052..5213)
                     /locus_tag="VEV43_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23306.1"
                     /translation="MRELFIDELAQVTGGMQCPVDCVTTDGCCEEGPYELCCVLQDVR
                     EAVSKLLPG"
     gene            5297..6208
                     /locus_tag="VEV43_07030"
     CDS             5297..6208
                     /locus_tag="VEV43_07030"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HYP23307.1"
                     /translation="MVPAAKMHVDEVDVDEALVRRLLGEQLPQWAELPLERVPSDGTD
                     NAIYRLGDDMAVRMPRYPSAAGQVTKEAQWVPRLAPHLPLDVPVPLALGEPGDGYPWR
                     WSVVTWLPGGRASFEALSDPGDAAARLAGFVRALERIDTEGGPPPGDHNFGRGAPLAT
                     RDGYVRDALAQLGSELDVGAAEAAWERALSAPEYSAPGVWIHGDLHGGNLLTNGGRLT
                     GVIDFGGLGVGDPACDVMPAWTFFRGPARATFRERLAVDDATWERGRGWALSMGLIAL
                     PYYRDTNPSLAGTARRWIEEVLTDRDG"
     gene            6234..6620
                     /locus_tag="VEV43_07035"
     CDS             6234..6620
                     /locus_tag="VEV43_07035"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYP23308.1"
                     /translation="MAARPDAVGAALVGSHARGQARADSDVDLMIVTTSPGGYLSDTS
                     WTRAFGETESVEVEEWGAVTSVRVRYVMGAEVEFGITTPEWTKTSPIDPGTKRVVQAG
                     FRILLDRDGSLRALHEECADHASYKD"
     gene            complement(6608..7333)
                     /locus_tag="VEV43_07040"
     CDS             complement(6608..7333)
                     /locus_tag="VEV43_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23309.1"
                     /translation="MNYEEFVPVMPEDDAMPKGLGRFPDEIDAVLSPLKERVRGLPTT
                     VAGERRKFAHYLLLDAMSSAHAIELVVKSSRVLGTPPLADAIEPVVRHVVEKAIVIAY
                     VARSNDERVVDRFLKTSAREWERSFGSAESHPHLDLPVKELPKYKPMAEAACPELVEV
                     FAKLSWISHPRSALPFSMQAYASADVTQYAQLRVAKALGWLEEALAVVLDAHDVINEE
                     EVDEPDTRPYFLSKNEFTRDQSL"
     gene            complement(7362..7799)
                     /locus_tag="VEV43_07045"
     CDS             complement(7362..7799)
                     /locus_tag="VEV43_07045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYP23310.1"
                     /translation="MKTIRKSIVIETARSAVFDLVDDPGCYPEFFVGITKWEPRSKKR
                     RGVGAKYRVLMQVGSIEAGGTVRVTERTVNRVIAWTWEQGIHQEGRWTLEDAGGATEL
                     SLEVRYDLSGGPLGRVVEHLTGRIVERNLWATLLAARRLLETA"
     gene            complement(7796..8632)
                     /locus_tag="VEV43_07050"
     CDS             complement(7796..8632)
                     /locus_tag="VEV43_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYP23311.1"
                     /translation="MSSIEAERKALIAGAAAELDARDPEFIRYQLPGMWLFSTMYFRA
                     EVTGFDRVPDGPVLFVGNHSGGNMTPDSMVFMLAFNTYFGVERPVYALAHSLVTSWPV
                     LGKLAQRWGIVTAGPKAARAALERGASVLVYPGGDVDTHRPWTARHEIRFDGRKGFLR
                     IARDAGVPIVPVVSVGGQDTYLPLTDGRKVAELLRLDKIARLKVLPVSLALPWGLNVG
                     DFLGHVPLPAKIRMDVLEPIDVAERFGDEADSDEAYDYVVSRMQESLTALAAGRLLPP
                     FL"
     gene            complement(8629..9789)
                     /locus_tag="VEV43_07055"
     CDS             complement(8629..9789)
                     /locus_tag="VEV43_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP23312.1"
                     /translation="MSSLEAALLDRLADPLDVGSRKRAKVALEVGPGDGWTFHADRGE
                     LLLSHGADDRADARITTDARTMVEILRGERSGIEAFIGGDLRVRGDLALTLRLSGSLP
                     GAVAPDRFPRGREAAAGGGTFYLEAGRGPDVLLLHGLGATNASMLTTLWDLAQDHHVI
                     APDLPGFGDSAKPLRGYDAPFYGKWVLDLTDELGIERAHVVGNSLGGRVAIEAALQDP
                     DRVDRLALLTPSPAFIRRREFARLVKLLRPEAAFVPLPLSHRRVVGSIKALFARPHRL
                     PDQWYEAAADEFLRVFSSPRGRIAFFSAARQIYLEEPYGDRGFWDRLQSLTRPSLFVW
                     GARDWLVPPRFARHVERAVPDASSVVLADCGHVPQFELPDRTHGIVREFLAA"
     gene            complement(9786..10535)
                     /locus_tag="VEV43_07060"
     CDS             complement(9786..10535)
                     /locus_tag="VEV43_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYP23313.1"
                     /translation="MEDGWMTIEELAHRTGMTVRNIRAHQTRGLLPAPAVRARTGYYG
                     PEHVARLEVVKEMQASGFNLAAIRHVLEMAPAGSGEELLRLERLLMAPWEEEEPEAVS
                     LEDLAERFGPIDPDTLRRTVALGVLRELGDGTFEVPSPALLRAGEEVVALGIPLDHAL
                     AVVEKVQRHAKGVAREFVRLFVQDVWRPFKAAGMPQEQLPEIRKSLEKLRSLASDVVL
                     TTFRLKMADETESAFGKELERPLTRRGRRAS"
     gene            10588..>10886
                     /locus_tag="VEV43_07065"
     CDS             10588..>10886
                     /locus_tag="VEV43_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23314.1"
                     /translation="MDTISTRPPLTPIEHFGAPGLDETPAAQLRAIREQAPAFKEWFR
                     STGTVDLFAARSLVTLPYPKRFALWEACNVPVPYVWMTNRMFVVQWLAEGRTKTLL"
BASE COUNT         1660 a         3834 c         3652 g         1740 t
ORIGIN      
        1 gaggccctcc gctgcgatca tgtggtcgcc gctctgcaag tacgtcgcca ggatcgtcgt
       61 catggcgctc atcccggacg cggtggccac ggcagcctcg gccccctcga gcgcggcgag
      121 cttgtcgtgg aggtaggcct gcgaaggcgt gttgctgaga cggatgtagt cgatgtcatc
      181 ggggtcgtcg gtgccgggct tcgtcgcgta gacggtgctc tggtagatcg ggaagacgat
      241 cgacccttcc gggcccggac gtcgttcgcc gccatggacg gacctggtgg cgtcacccac
      301 ccttcggctt gccggcatcg ttcgacggta cccagacggg gacctcggga accggcgacc
      361 tctcggaaac gaaccggtcc gagagccccg gcgcgcgcgg caccgcgacg gtcacgcggt
      421 gcctctcacg caggcgcgtc cggcacgccg gcattcttcg cgcggcccct ctcggacgag
      481 cctcagcgca aggatcgcca gctcgtacat gacgaagagc gggcccgcca gcgcgcgctc
      541 gtcgcgatcg tggctctggc cgcggtggtg acgccgagcc aggatcccta cacgctcctg
      601 gcgcttgcgg aagggtcgca ccgggggttc ggaagccggg ggcctcgggg attgcgcggc
      661 ggacctatcg ctgcagctcg gtgctggatc gtagggcgtc cgtcccctcg agcgcccaat
      721 ccgtcagcct gtcctgcggc tcccggctag cagcggccgg gcggcagact caggttccgc
      781 aggcactcca tgaccctctg gtcccagcac tggatcactg ccgtcgcgcc cttgcactcc
      841 ggcgcggctt gggccgccgg tgcgacgacc gatccggctc ccagcaccaa cgctactgcc
      901 gctatcgtcc gcgccaacct cgtccgcgtc atgatcttgc cctccccttg gtcgtggtcg
      961 attcgccgag gcgagcaaat cacacctgcc gcccgtgggc caattcgggc tcaaagggcc
     1021 cagacgggct attgccgggg cttcaccccg gcccccatcc tgaggaacca ggactcgccg
     1081 agggggcctt tcttgactgg ttggtgttct ccgcgcgctt tgcgcttggc gtcggtcgtc
     1141 gtgctcgcgg ccctcgccgt gtctctcgtc gcccaggacc gtgcccgcgg gtggcacacc
     1201 gaggcgtggg tcgcgccgct cctcgacagg gacggctaca ccctcaacgt cgtaagggga
     1261 gacgggtacc tcgcgtggac cgaggccggc atctgttgcg cgtcggcgat gcaatatgcg
     1321 aacggagccg ggatcggccc cgagcacgcg cggacgttca aagtgatgct gcgcaccccc
     1381 ggcggcaaga ccgtccagct gagccgggac ggcgtatggg cgatggccgg agggatcgac
     1441 cgaggcgtcc tcgcgtatgc gaccgggaag ccgcgcgcgg acatccgtct gctcgacctg
     1501 aagacgcgga agcaccggcg gttgccgcgc ggcgtgaaca cgaacctcgt cgaataccac
     1561 cccaccctct cgggaaagtg gctcctgttc ggacgcatac gtccctctcc gaggccgaac
     1621 gacgccatcc gcaaccgcac ttcggtgatc ctcacgaacc tcgagaccgg ccgttcccga
     1681 gtgctcgcag agggcaagaa ctaccgcagc tacacctatc ccggccaggt caacggggac
     1741 tggctcacgt acaccgcatg caagttcaac aagtacgggc ccaactgcgg cgtgactcgc
     1801 taccggatct cttccggcaa acgagcgagc gccgacctgg acggattctg ctgcaagcac
     1861 ggcgaccctc atacggagaa ccacatgtcc gcgccgggga gcgtccgggc ctccgccgtc
     1921 accgacgacg gtctggtcta catggcgatg acgagcgggc agaacacgtc gtggtgcggt
     1981 tacacggagg tctgggcctg ggaccgcgag gcccgcctcg cgtggatgtt cggcgagtac
     2041 gagggccggt cggccgacaa cccctacggg ggcctagtgg tcacgtcgct ctacgtcgac
     2101 gggagccggg tcctgttcga cggctacacg acttggatgg gcgaggatcc ggttcaccat
     2161 taccccacct gctacgtgga gacctcggcc ctcttcgtga tgggccacgg ctcgcctccg
     2221 ccgtcggctg cgacgccacc gcccaccccg acaccgtcgc cgtccgcgac cgagcctgcc
     2281 gagaccccca cccctacacc cacgccgact ccggaaccga ccccgacccc gaccccgacc
     2341 cccacgccgt ccccgtcacc atctccgagc cccgctccga gctgtccgct cccggtgtgc
     2401 gtctgagcgc ttagcctcgg ctccgtgctc gagaacgtcg tcgtagggat cgtcatggtc
     2461 gtcgggctgg ccggggtagt cgtgcccggc ctcccgggga ccctcctcat cctcgccgcg
     2521 ggtgtcggat gggcgttctt ccgcgacggc ggggccgggc cgtgggtcgt cgtcggcttc
     2581 atggcagcgc tcttcgtgct cggcactatc ctcaagtacg tcctgccggg acggcggctc
     2641 tcgggagagc tcccgcgctc gacgatcttc tccggagtcg tcggcgcgat cgtgggcttc
     2701 gtcgtgctgc ccccgctcgg ccttctcgtc ggcggtgtgg tcggcgtcta cctcgcggag
     2761 acccgtcgag tcgggccggg ggccgaggcg cggcgctcga ccgtcgcggt gctcaaggcc
     2821 attggcgtcg gcatcctcgc cgagctgacc gcgggcgtgc tgatgatcgt cacgtggctc
     2881 gtgggcctgg ccgcgaccta gaacccggcg actatcgact cgaggatgtt cccgtggcgc
     2941 acgcgctgcg ggtggtctcc gaccgggacc tcggccacga ccttctcctt gccgaacgag
     3001 atgatcgtga cgaggtcgct gccgctcatc gacatccagc acgtgttgcc cgggccctcg
     3061 accgcccaat agggcttcga gtactcgacg tcctcgacga agcgggccgc tccgtcgaag
     3121 accttcggcg tgaacgtctt gcgatcgacg ctcgcggcgt agtcggacat ggtcccggcc
     3181 acacagagct tgttcccgcg cgcgttcatc gcgatcccgt ggtgcgcgga gtcgagcacg
     3241 tactgctcgc gtggcatgtc cttcacgtcc tcagcgatcg ggaggtcgac gatgcgcgtc
     3301 acgacaccgg tcttcggctc cgccaggccg ccgagctcgt agtcgccgcc tcccgtctcg
     3361 tccttcttgt tcaagtggaa ctcgacgaac ccgtggaaga acgacacctg cgcgtacatg
     3421 atcttctcgt cgggggtgag ggcgaccgga cggaccgcgc tgctcatgtc cgggtggccg
     3481 gcctcggcga gctcctggcc catgtcccag cgtcgcttca cctcgaagct gtcgttgtcg
     3541 actacctgga gccagcggtc gcccttgctc gtatcccctc cgctggagtc ggagtcgcct
     3601 tcgtcgcccg gggtgtagac gcgtccgatc gaggcgtgga agatccgtct cccgtcggcc
     3661 gagtagttgt tctcgtgcgg agtgtcgccg gactcgaacg accgcaggcg ttcgcccatc
     3721 gtcacctcgg tcccgtcggg aagcgtctcg tccaccatgc tgaactcgac gacctgtcgc
     3781 gcggtcgagt ccgagacgac caggcgccgg ccgtcgggcg agacccccat gtggtcggtg
     3841 cggtagccgt ccatctcggc ctccgcgacg atgctggcgg gatcgccggc ctctcccgcc
     3901 gcggccgcct tggcgaggtc gatccacacg acgtcgcgta ggctcgggcg cgagaccgcg
     3961 aggtacttcc cgtcgatcgt tgtgaaggag tcgtcgacgt actggtcgtt gccctcaccg
     4021 acgccctgct ggatcgcgac gtagaacgcg ggcccggcgg ggtggctcgg cgagggtgtg
     4081 tttacgtgtt gcagctcttc gtccttgtcg gggacgacgt tgatcgtcgt gaggacctcc
     4141 cgggaacggg cgtcgacgat cgtcgccgtg ccggcccagt tgttgctgac gaccatgacg
     4201 cttcgggtgg cggggtcctc ctgcgcggcc cccacgcccc cggccggtac gcctcctgcc
     4261 agggtggccg cggcgatcgc cacgagacct atgacgagcg gacctctccc tgcgcgcacg
     4321 acgacctccg atgcccgtga acccggttca ccggagacgt tacttggagc ggccgggctg
     4381 cggacgacgg cgtggccgcg cctctgactg ggtcgacaca gggcgaccca gcgagcggac
     4441 tagtgtggcg acgactcatg ccgacgatcg aagagcgccg cttccaagac cgtgagctga
     4501 ccaacgaaga agtacagggc tccgagttcc gcaggtgccg gttctccgga gtcgtcttct
     4561 cggacgacga gtacacggac gcgaccttcg agcactgcaa gttcctgagc tgcgacttct
     4621 cctcgacccg tcaccgcaac gcctcgttcc gcaactgtga cttcgccggg tcctcgctcg
     4681 cctcgacccg tttcgacact tgtcgcctca tggggtccga cctgagcgag gcgcggctgt
     4741 ggaacgtcga gtgcgacgga ggcaactggt cgtacgtgaa cctgcgcatg caggagctgg
     4801 ccggctggcg ggcccccggg atcgttctcc gcggcgccga cctctaccag gccgacctga
     4861 ccggcgccga cctcacgggc gcggatctca ccgaggcgaa cctcgacaag gtcaagctga
     4921 aggacgccga cctacgcggc gcgaacctga cgcgcacgaa cctcgcggag ctcgtcctgg
     4981 acaagacccg gctcgacttc gagcaatgcg tcctcttcgc ggtcgcgcac ggcgcccgcg
     5041 tggactgacg gctagcccgg aaggagcttc gagaccgcct cgcggacgtc ttgcaggacg
     5101 caacagagct cgtaggggcc ctcttcgcag catccgtcgg tcgttacaca gtcgacgggg
     5161 cactgcatgc cgccggtcac ctgcgcgagc tcgtcgatga acagctccct catatccgcc
     5221 tccttcgtga tcgtctgccg gggggatcgt accgacgcga gcccggtcgc gcccgagccg
     5281 ggtacggtct cgcgtgatgg tgcccgcggc gaagatgcac gtcgacgagg tcgacgtcga
     5341 cgaggcgctc gtccgccggc tgctcggcga acagcttccg cagtgggcgg agctccctct
     5401 cgagcgcgtg ccgtcggacg ggaccgacaa cgcgatctac cggctcggcg acgacatggc
     5461 ggtgcggatg ccccggtacc cgagcgcagc cggtcaggtg acgaaggagg cgcagtgggt
     5521 gccacgtctc gccccgcacc ttccgctcga cgttcccgtt ccgctcgcgc tcggcgagcc
     5581 gggcgacggc tacccgtgga ggtggtcggt cgtcacgtgg ctgccgggcg ggcgagcgtc
     5641 gttcgaagcg ctctcggacc ccggcgacgc ggcagcacgg ctggcgggct tcgtccgtgc
     5701 ccttgagcgg atcgacacgg agggcggccc tccgcccggc gaccacaact tcggacgagg
     5761 agcgccgctg gcgacgcgcg acggttacgt gcgcgacgcg ctcgcgcagc tgggaagcga
     5821 gctcgacgtc ggcgccgcgg aggcggcatg ggaacgcgct ttgtccgcgc ccgagtactc
     5881 ggcccccggg gtgtggatcc acggagacct tcacggcggc aacttgttga cgaacggcgg
     5941 gcggctcacg ggcgtgatcg acttcggggg cctcggagtc ggcgacccgg cctgcgacgt
     6001 catgccggcg tggacgttct tccgtggtcc ggcccgggct acgtttcgcg agcgcctcgc
     6061 ggtggacgac gcgacgtggg agcgcggtag gggctgggcg ctatcgatgg ggctgatcgc
     6121 gcttccgtac taccgggaca cgaacccgtc gctggccggg acggcgcggc gctggatcga
     6181 agaggtgctg acggatcgag acggctgagc tgatcgaacg cttcaccgaa tgggtggccg
     6241 cgcggcccga cgcagtgggg gccgcgctgg tcgggtcgca tgcgcgcgga caggctcgtg
     6301 cggactccga cgtcgacctg atgatcgtga cgacctcgcc gggtgggtac ctgagcgaca
     6361 cgtcgtggac ccgcgccttc ggcgaaacgg agtcggtcga ggtcgaagaa tggggagcgg
     6421 tgacgtcggt gagggtccgc tacgtcatgg gggccgaggt cgagttcggg attacgaccc
     6481 cggaatggac gaagacgagc ccgatcgacc ccgggacgaa gcgcgtcgtg caagccggct
     6541 tccgcatcct gctcgaccgc gacggctcgc tacgtgcatt gcacgaggaa tgtgcggacc
     6601 acgctagtta caaagattga tcccgggtga actcgttctt gctgaggaag tacggacgcg
     6661 tatccggctc atctacctcc tcctcgttaa tgacatcgtg tgcgtcgagt acgaccgcaa
     6721 gagcctcctc caaccatccc agagccttcg caactcgaag ctgcgcgtac tgcgtcacgt
     6781 ccgcacttgc atatgcctgc atggagaaag gtagggcaga cctgggatgt gaaatccacg
     6841 atagcttcgc gaacacctca acgagctcag ggcatgcggc ctcggccatc ggcttgtact
     6901 tagggagctc cttcacaggc aggtcgaggt gtgggtgcga ctcagccgat ccgaacgagc
     6961 gctcccactc tcgggcactt gtcttaagaa accggtctac gacccgttcg tcattagatc
     7021 gtgcgacata cgcgatgaca atcgctttct cgactacgtg gcgtacaacg ggctcgatcg
     7081 cgtcagctaa gggtggagtg cctaggactc gcgacgattt gacaactagc tcgatcgcat
     7141 gagccgagct catcgcgtcc aaaagcaggt aatgggcgaa cttccggcgc tcacccgcca
     7201 cagtcgtagg aagacctcgg acgcgctctt tgagcgggct caaaacggcg tcaatctcgt
     7261 ctgggaagcg ccccaatcct ttcggcatgg cgtcatcttc cggcatgact gggacgaact
     7321 cctcatagtt cactcagccg gagcgccccc gcgacggctc gttacgcggt ctccagcagc
     7381 ctgcgcgccg ccaggagcgt cgcccatagg ttccgctcga ctatgcgacc cgtcaggtgt
     7441 tcgaccaccc ggcccagcgg gcccccgctc aggtcgtagc gcacctccaa cgacagctcg
     7501 gttgcgcccc cggcgtcctc gagcgtccag cggccctcct ggtggatccc ctgctcccac
     7561 gtccacgcga tgacgcggtt caccgtccgc tccgtcacgc gcaccgtccc gccggcctcg
     7621 atcgacccga cctgcatcag cacccggtac ttcgcaccga cgccgcgccg cttcttcgaa
     7681 cgcggctccc acttcgtgat gccgacgaag aactcggggt agcagccggg atcgtcgacg
     7741 aggtcgaaca ccgcactgcg cgcggtctcg atcacgatgc tcttgcggat cgtcttcaca
     7801 ggaacggggg caggagccgt ccggccgcga gcgcggtcag gctctcctgc atgcgcgaca
     7861 cgacgtagtc gtaggcctcg tcggagtccg cctcgtctcc gaagcgttcc gcgacgtcga
     7921 tcggctcgag gacatccatc cggatcttcg ccggcaacgg gacatggccg aggaagtctc
     7981 cgacgttgag cccccatggc agcgcgagag agaccggcag caccttgagc cgcgcgatct
     8041 tgtcgagccg caggagctcg gcgaccttcc gcccgtcggt caagggaagg tacgtgtcct
     8101 gcccgcccac ggacacgacc ggcacgatgg gaacgccggc gtcgcgcgcg atgcgcagga
     8161 accccttgcg cccgtcgaag cggatctcgt gacgcgcggt ccagggccgg tgcgtgtcga
     8221 cgtcaccgcc cggatagacg agcacggacg ctccgcgctc caaggccgca cgcgcggcct
     8281 tggggcccgc cgtgacgatc ccccaccgct gcgcgagctt cccgagcacg ggccacgacg
     8341 tcacgagcga gtgcgcgagc gcgtacaccg gccgctcgac gccgaagtag gtgttgaacg
     8401 cgagcatgaa caccatgctg tcgggcgtca tgttcccgcc ggagtggttt ccgacgaaca
     8461 gcacaggtcc gtccggcacg cggtcgaagc cggtgacctc ggcccggaag tacatcgtcg
     8521 agaacagcca catccccggg agctggtagc ggatgaactc tgggtcgcgg gcatcgagct
     8581 cggcggccgc ccctgcgatg agcgccttgc gctccgcttc gatcgagctc acgccgcgag
     8641 gaactcccgg acgatgccgt gggtgcgatc cggcagctcg aactgcggga cgtgcccaca
     8701 gtcggcgagc acgaccgacg acgcgtcggg caccgcgcgc tcgacgtggc gcgcgaaacg
     8761 cgggggcacg agccagtcac gagcgcccca cacgaacagc gagggccgcg tcaggctctg
     8821 cagacggtcc cagaacccgc ggtcgccgta gggctcttcg aggtagatct gccgggccgc
     8881 cgagaagaac gcgatccgcc cgcgcggcga cgagaacacg cggaggaact cgtccgctgc
     8941 ggcctcgtac cactggtccg ggagccggtg cgggcgtgcg aacagcgcct tgatcgaacc
     9001 gacgacgcgc cggtgggaca gcggcagtgg gacgaacgcc gcctccggcc gcagcagctt
     9061 cacgagccgt gcgaactcgc gccggcggat gaatgccggc gagggagtca ggagcgcgag
     9121 cctgtcgact cgatcggggt cctgcagtgc cgcttcgatc gcgacgcggc ccccgagcga
     9181 gttcccgacg acgtgggcgc gctcgatccc cagctcgtcc gtcaggtcga ggacccactt
     9241 cccgtagaac ggcgcgtcgt aaccgcgcaa cggcttggcc gagtcgccga atcccgggag
     9301 gtccggggcg atcacgtggt gatcctgagc gaggtcccac aacgtcgtca gcatcgacgc
     9361 gttcgtcgcg ccgagcccgt gcagcaggag cacgtccggc ccccgccccg cttcgagata
     9421 gaacgtcccg ccgccggctg cggcctcgcg cccgcggggg aaccggtccg gcgcgaccgc
     9481 gccgggaagg ctcccgctga gacgcaacgt cagcgcgagg tcgccgcgca cgcgcaggtc
     9541 cccgccgatg aacgcctcga tgcccgaccg ctcgccgcgc aggatctcga ccatcgtccg
     9601 cgcgtcggtc gtgatccgtg cgtccgcgcg gtcgtcggcg ccgtgcgaca gcagcagctc
     9661 gccgcggtcg gcgtggaacg tccagccgtc gcccggcccg acctcgagag cgaccttcgc
     9721 ccgcttgcgc gacccgacgt cgagcggatc ggcgagccgg tcgaggagcg cggcctccag
     9781 cgagctcacg aagcgcgcct gccgcgccgc gtcagcgggc gctcgagctc cttgccgaac
     9841 gcgctctcgg tctcgtccgc catcttcaga cggaacgtcg tcagcacgac gtccgacgcg
     9901 agcgaacgga gcttctccaa cgacttcctg atctcgggca gctgctcctg cggcatcccc
     9961 gcggccttga acgggcgcca cacgtcctgc acgaacaacc tgacgaactc gcgtgcgacg
    10021 cccttggcgt gacgctgcac cttctcgacg accgcgagcg cgtggtcgag cgggatcccg
    10081 agcgcgacca cctcttcccc cgcgcggagc aacgcggggg aagggacttc gaacgtcccg
    10141 tcgcccagct cgcgcagcac cccgagcgcg accgtccgcc gcaacgtgtc ggggtcgatc
    10201 gggccgaagc gctcggcgag gtcttcgagc gacaccgcct ccggctcctc ttcctcccag
    10261 ggcgccatca gtaggcgttc gagccggagc agctcctcgc ccgagcccgc gggggccatc
    10321 tcgaggacgt ggcggatcgc ggcaaggttg aagcccgacg cctgcatctc cttgacgacc
    10381 tcgaggcggg cgacgtgctc gggcccgtag tagccggtcc gggcgcgcac ggcgggtgcg
    10441 ggcagcaacc cgcgggtctg gtgggccctg atgttcctca ccgtcatgcc ggtccggtgg
    10501 gcgagctctt cgatcgtcat ccagccgtct tccacgggag gaatattacg ccgtttaggg
    10561 taacatcaaa cggtgtaaca taggctcatg gacacgatct ccacgcgccc cccgctgaca
    10621 ccgatcgagc acttcggggc ccccggtctc gacgagaccc cggcggcgca gctccgggcc
    10681 atccgcgagc aggcccccgc gttcaaggag tggttccgct cgaccggcac cgtcgacctc
    10741 ttcgcagcgc gctcgctcgt cacgctcccg tacccgaagc ggttcgccct gtgggaggcc
    10801 tgcaacgtcc ccgtccccta cgtgtggatg acgaaccgca tgttcgtcgt ccagtggctg
    10861 gcagagggcc ggacgaagac gctgct
//
LOCUS       DASQDO010000246         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1187221, whole genome shotgun sequence.
ACCESSION   DASQDO010000246 DASQDO010000000
VERSION     DASQDO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187221"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..716)
                     /locus_tag="VEV43_07070"
     CDS             complement(54..716)
                     /locus_tag="VEV43_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP23315.1"
                     /translation="MPKILVVEDQQSVVAIVRYHLESAGMEGFFASDVGDGWRLLTAE
                     APDAAVIDIGLPGADGWTLIEKLRGDDRFARLPVVVLTGLIEPDVVRRAASLGCEYLS
                     KPFAASALLGKIRAGLQPGLVTARPEPRPDPNKVELVVVGVVMLLDHYQVEGRVHLPP
                     ELARFSDAWESLIRDNREYVPVTDVRVTLRDGHTVVASPSFMEVRKTDVRAVFPQDAP
                     VG"
     gene            859..1482
                     /locus_tag="VEV43_07075"
     CDS             859..1482
                     /locus_tag="VEV43_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23316.1"
                     /translation="MVQRKPTAAVAVIVVLLLSAVVAAIALATTSQDAVAQQGCTGGG
                     SSPSPSVSPSPSESEEDPIPSILPSLLPDQAPKKAEGKAADPAPLPAPDKDGSEAADA
                     ADQQQRSCRSTITLAYKSGRNPKFTGTVDSDEPMCKRARNVTIKKVKRGADPRVAKTT
                     TNTKGKYTAPARKANGRFYAKVSKATTENRDGETVSCGAARSRAIKP"
     gene            complement(1535..>2721)
                     /locus_tag="VEV43_07080"
     CDS             complement(1535..>2721)
                     /locus_tag="VEV43_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYP23317.1"
                     /translation="CSEGAPIEPAAAPEPPALDAAAPAPAPRALAPEDVTSRLRQVAA
                     GLSSPLGVTGAGDGRRWLYVVEQEGRIRIFREGKLRDTPFLDVSDRTEGGGEQGLLGL
                     AFHPDYETNRRFFVNYTDLNGDTVIAEYRRARRNPARALRGSERVLLRVDQPFSNHNG
                     GALAFGPDGMLYIALGDGGSAGDPQNNGQRKDTLLGKILRIDVSSRATEYEIPDDNPF
                     TGDPTARGEIWDYGLRNPWRMSFDRDTGALWIADVGQGEWEEVNREPAGSGGLNYGWR
                     VKEGRDCYPAGEECEIVGGALEDMTDPLAVYSHDEGCSVTGGFVYRGRAFAELTGNYF
                     FGDFCSGRIWAVAAGGPDRQRPVELLDTDLSISSFGEDDRGELYVTDLAGGGLYRLRP
                     AS"
BASE COUNT          427 a          994 c          926 g          374 t
ORIGIN      
        1 gatgccgagc gactcgtgga tcgtgttctc gtagacgatc ccgccgccgt cgctcaaccg
       61 accggcgcgt cctgcgggaa gaccgcgcgc acgtcggtct tgcggacctc catgaacgac
      121 ggagacgcca cgaccgtgtg gccgtcgcgc agcgtcaccc tcacgtcggt cacgggcacg
      181 tactcgcggt tgtcccggat cagcgactcc cacgcgtcgg agaaacgcgc cagctcgggc
      241 ggcaggtgca ccctgccctc gacctggtag tggtccagca gcatcacgac gcccaccacg
      301 acgagctcga ccttgttcgg gtccgggcgc ggctcgggcc gcgccgtcac gagccccggc
      361 tgcaacccgg cgcggatctt gccgagcagc gcggaggcgg cgaacggctt gctgaggtac
      421 tcgcatccga gcgaggcggc ccgccggacg acgtccggct cgatcagccc ggtgagcacg
      481 acgaccggga gccgggcgaa gcggtcgtcg ccgcgcagct tctcgatcag cgtccagccg
      541 tctgcccccg gcagcccgat gtcgattacg gcggcatccg gcgcctcggc cgtcagcagg
      601 cgccacccgt cccccacgtc cgaggcgaag aacccctcca tcccggcgct ctcgaggtgg
      661 tagcgcacga tcgccacgac ggattgctgg tcctcgacga ccaggatctt cggcatcccg
      721 acatcatcct ccaagccgcg tatgccagac cttgccagta cggtgtggtt gaaccctgct
      781 acacagggaa accacatagt cacgagttcg ccactaagat caggtcaaac gagacaaaat
      841 caccctaggg ggtgcccgat ggtccaaaga aaacccacgg cggccgtagc ggtcatagtc
      901 gtcctgctgt tgtcggcggt cgtagccgcc atcgcactgg cgacgacgag ccaggacgcc
      961 gttgcccagc aggggtgcac cggaggcggc agctcgccgt ccccgtccgt ctcgccgtcg
     1021 ccgtccgagt ccgaggaaga cccgatcccg tcgatcctcc cgtcgctgct cccggaccag
     1081 gcgccgaaga aggccgaggg gaaggccgcc gaccctgctc cgctcccggc ccccgacaag
     1141 gacgggtccg aggcggcgga cgcggcggac cagcagcagc ggagctgccg ctcgacgatc
     1201 acgctggcct acaagtccgg caggaacccg aagttcaccg gcacggtcga ctcggacgag
     1261 cccatgtgca agcgggcccg caacgtgacg atcaagaagg tcaagcgggg tgccgacccg
     1321 agggtcgcca agacgacgac gaacaccaag ggcaagtaca cggccccggc ccgcaaggcg
     1381 aacggccgct tctacgccaa ggtgtcgaag gcgacgaccg agaaccgcga cggcgagacc
     1441 gtctcctgcg gcgccgcccg gtcgagggcc atcaagccgt aacgaggacc agtatccggc
     1501 gagaggggca cggcgacgtg cccctcttcc gcgtctagga agcggggcgc agccggtaga
     1561 gaccgccgcc ggcgaggtcg gtgacgtaga gctcgccgcg gtcgtcctcg ccgaacgagc
     1621 tgatcgacag gtcggtgtcg agcagctcga ccggccgctg ccggtccggc cctcccgccg
     1681 ccacggccca gatccgcccc gagcagaagt cgccgaagaa gtagttgccg gtgagctcgg
     1741 cgaacgcccg gccccggtac acgaagccgc cggtcaccga gcagccctcg tcgtgcgagt
     1801 acaccgccag cgggtccgtc atgtcctcga gcgcgcctcc gacgatctcg cactcctcgc
     1861 ccgccgggta gcagtcgcgg ccctccttga cccgccatcc gtagttcagc ccgcccgagc
     1921 cggcgggctc gcggttgacc tcctcccact cgccctggcc gacgtcggcg atccacagcg
     1981 cgccggtgtc gcggtcgaac gacatgcgcc aggggttgcg cagcccgtag tcccagatct
     2041 cgccccgggc ggtcgggtcg ccggtgaacg ggttgtcgtc cgggatctcg tactcggtgg
     2101 cgcggctcga gacgtcgatc cgcaggatct tgccgagcag cgtgtccttg cgctgtccgt
     2161 tgttctgcgg gtctccggcg gagccgccgt cgccgagcgc tatgtagagc atcccgtccg
     2221 gcccgaaggc cagcgcaccc ccgttgtggt tcgagaacgg ctggtcgacc cgcaggagca
     2281 cgcgctcgct cccgcgcagc gcccgggcgg ggttgcggcg cgctcgccgg tactccgcta
     2341 tcaccgtgtc cccgttcagg tcggtgtagt tgacgaagaa cctgcggttc gtctcgtagt
     2401 ccgggtggaa ggccagcccg agcagcccct gctcgccccc gccctcggtc cggtcggaga
     2461 cgtcgaggaa cggcgtgtcc cggagcttgc cctcgcggaa gatccggatc cgtccttcct
     2521 gctcgacgac gtagagccag cgccgtccgt cgcccgcgcc ggtgacgccg agaggcgaag
     2581 acaggcccgc ggcgacctgc cggagacggc tcgtgacgtc ctccggggca agggcccgcg
     2641 gcgccggggc gggggccgcg gcatcgagtg cgggcggctc gggggccgcg gcgggttcga
     2701 tcggtgctcc ctcgctgcac g
//
LOCUS       DASQDO010000247         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_820221, whole genome shotgun sequence.
ACCESSION   DASQDO010000247 DASQDO010000000
VERSION     DASQDO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820221"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VEV43_07085"
     CDS             complement(<1..645)
                     /locus_tag="VEV43_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP23318.1"
                     /translation="MNVVVTGAAGGIGTAAVRALREHGARVAGIDLLDEGDLVRGDVT
                     DRDSIAAAVETCAASLGGVDVLVNNAGIGVPGDAGDFPDAAAHAVREVNFFGAWNATA
                     AALPDLLESRGHVVNVASGLAIVDVPFAAAYSASKRALAAYSAALRVEYRGRIGVTTV
                     YPGYIRTSIHDAAAHAVMEVNFFGAWNATAAALPYLLESRGHVVNVASGLAIFDV"
     gene            complement(642..2408)
                     /locus_tag="VEV43_07090"
     CDS             complement(642..2408)
                     /locus_tag="VEV43_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23319.1"
                     /translation="MILAHGNEVGFGTFGTDWLYLLVPVAALLAIVGWLYATGERSGG
                     GFVTATVTRIGSSLERVTGLPSWAAAGLGVAAWSLVVAVIGFLWDVAWHIDFGRDEFL
                     FTPSHMMILVGLGAIIAAAVVSILFATVLRAETGWRLRSLRVPYGAAALGVLGTGAIL
                     GFPLDELWHANYGIDVTMWGPTHLLMISGASFTPLGMWLLLREALPQARNEAFVRMLR
                     FLLAGVVVVGLSTFQAEFDFGVPQFQQLYHPVLIALATGVGLVIARTTLGPGGAIAGA
                     LGFLAVRGALVVLLGPVFNETVARPPLYLGIAVVVEVAAFAGRRLSPLKSALVTGAAV
                     GTIGLATEWGWTQVFGVHPWSTNLFPGIAVAVVVAVAAAIVGTAMGRILACERPGVGR
                     VPLALAGLAILVGLILPLPRRAAPYRGTIEMTPAGDGRVDLTVRMDPAEAADRVDFFE
                     VMSWQGGSLVVTELERRGPGVYESATPVPADHHWKSLVRLADKDLMAGLPVFLPADPA
                     IGAKEIPVVAERTAAFNRDTELLMREAHAGAAWPATVAYSSILGIALVWIVTLTLGFV
                     RLGGSPQRQERTSRATSRPVTT"
     gene            complement(2405..3106)
                     /locus_tag="VEV43_07095"
     CDS             complement(2405..3106)
                     /locus_tag="VEV43_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23320.1"
                     /translation="MRHAHVDEHLDQLGISPADVDYLAFDHLHTQDIRRLIGTTAPAP
                     DLGYAEGPVPATFPNAKLIVQPSELEHVRDVHPFQARFHQSWTYGDVNEDALLVVDGD
                     VLLGPGVALLRTPGHTLGNHTLVVNTANGIFTSSENGVSVECYAPHHSKIPGVAKWAR
                     EWGYEVVMNFNTPEYASWQYNSMVKEKLVADPIAGRPELPQVFPSSELTRHRLAPGIR
                     TTFEHGDVTIGAVRQ"
BASE COUNT          532 a         1175 c         1118 g          437 t
ORIGIN      
        1 gacgtcgaat atagccaggc ccgacgcgac gttgacgacg tgtccgcggc tctcgagcag
       61 gtacggaagc gcggcggccg tcgcgttcca cgcgccgaag aagttgacct ccatcaccgc
      121 gtgggccgcg gcgtcgtgga tcgacgtcct gatgtagccg gggtagaccg tcgtcactcc
      181 gatgcggccc cggtactcca cccgcagcgc cgccgagtac gccgcgagcg cgcgcttgct
      241 cgcgctgtac gccgccgcga acgggacgtc gactatcgcc aggcccgacg cgacgttgac
      301 cacgtggccg cggctctcga gcaggtccgg gagcgccgcg gccgtcgcgt tccacgcgcc
      361 gaagaagttg acctccctca ccgcgtgggc cgcggcgtcg gggaagtcgc ccgcgtcgcc
      421 ggggactcct atccccgcgt tgttgacgag gacgtctacc ccgccgaggc tcgcggcgca
      481 ggtttcgacc gcagccgcga tcgagtcgcg gtccgtcacg tctccccgga cgaggtcgcc
      541 ttcgtcgagc aggtcgatgc cggccactcg ggcgccgtgc tcgcgcaggg ccctcacggc
      601 agcggtgcct atgcccccgg ccgcgccggt gactacgacg ttcaagtcgt gacgggacgc
      661 gacgtcgcgc ggctcgtgcg ctcctgccgc tgcggcgagc caccgagccg cacgaacccc
      721 agtgtcaacg tcacgatcca cacgagcgcg atcccgagga tcgacgagta cgcgaccgtc
      781 gccggccagg ccgcgcctgc gtgggcctcg cgcatcagca gctcggtgtc gcggttgaac
      841 gccgcggtgc gctcggctac gaccgggatc tccttcgccc cgatcgccgg gtcggccggg
      901 aggaacaccg gcagccccgc catcaggtcc ttgtcggcca gccggacgag cgacttccag
      961 tggtggtccg ccgggacggg ggtggccgac tcgtagacgc ccggcccccg tcgctcgagc
     1021 tcggtcacga ccagcgatcc gccctgccac gacatgacct cgaagaagtc gacgcggtcc
     1081 gccgcctcgg cgggatccat ccgaacggtg aggtcgaccc gcccgtctcc ggccggggtc
     1141 atctcgatcg tcccccggta gggggccgcg cgccgcggca acggcaggat cagcccgacg
     1201 aggatcgcga gcccggcgag cgcgagcgga acccgcccga ccccgggccg ttcgcaggcg
     1261 aggatccgtc ccatcgcggt cccgacgatc gcagcggcga ccgcgaccac caccgcgacg
     1321 gcgatgcccg ggaagaggtt cgtgctccac gggtgtactc cgaagacctg ggtccagccc
     1381 cactcggtcg cgagcccgat cgttccgacc gcggcccccg tgacgagcgc gctcttcagc
     1441 ggcgacagcc tccgtccggc gaaggcggcg acctcgacga cgactgcgat ccccaggtag
     1501 agcgggggcc gcgcgacggt ctcgttgaag accggcccga gcagcacgac gagcgcgccg
     1561 cgcacggcga ggaaacccaa agctccggcg atcgcaccgc cgggcccgag cgtcgtgcgc
     1621 gcgatcacca agccaactcc ggtcgctagc gcgatcagca ccgggtggta gagctgctgg
     1681 aactgcggga caccgaagtc gaactcggcc tggaacgtcg acaggccgac gacgaccacg
     1741 cccgcgagca ggaacctcag catccgcacg aacgcctcgt tgcgcgcttg cggcaacgcc
     1801 tctcgcagca gcagccacat ccccaagggt gtgaacgacg ctcccgagat catcagcagg
     1861 tgcgtcggcc cccacatcgt cacgtcgatc ccgtagttcg cgtgccagag ctcgtccaga
     1921 gggaacccga ggatcgctcc ggtaccgagc acgccgagcg cggccgcccc gtacgggacg
     1981 cgcaacgaac gcagccgcca cccggtctcg gcgcgcagca cggtcgcgaa caagatcgac
     2041 acgacggccg cggcgatgat ggccccgagg ccgacgagga tcatcatgtg cgagggcgtg
     2101 aacaggaact cgtcgcgtcc gaagtcgatg tgccaagcga cgtcccacag gaacccgatg
     2161 accgcgacga ccagggacca cgccgcgaca ccgaggccgg ccgccgccca cgaggggagc
     2221 cccgtgacgc gctcgagaga cgagccgatg cgcgtgaccg tcgcggtgac gaaccctccg
     2281 cccgaccgtt ctccggtcgc gtacagccag ccgacgatcg cgagcaacgc ggcgaccggg
     2341 accagcaggt acagccagtc cgtgccgaac gtgccgaagc cgacctcgtt gccgtgagcg
     2401 aggatcattg gcgcaccgcc ccgatcgtga cgtcgccgtg ctcgaaggtt gtcctgatgc
     2461 cgggcgcgag acggtgacga gtcagctcgg aggaagggaa gacctgcggg agctcaggcc
     2521 ggcccgcgat cgggtcggcg accagcttct ccttgaccat cgagttgtac tgccagctcg
     2581 cgtactcggg cgtgttgaag ttcatgacga cctcgtagcc ccactcgcgc gcccacttcg
     2641 cgacgccggg gatcttcgaa tgatgcggcg cgtagcactc gacggagacg ccgttctccg
     2701 acgacgtgaa gatgccgttc gcagtgttga cgacgagcgt gtggttgccg agcgtgtggc
     2761 cgggcgtccg caacagcgcg acccccggac cgagcagcac gtcgccgtcg acgaccagca
     2821 gcgcgtcctc gttcacgtct ccatacgtcc agctctgatg gaaacgcgcc tggaacgggt
     2881 ggacgtcgcg cacgtgctcg agctccgacg gctggacgat cagcttcgcg ttcgggaacg
     2941 tcgccggcac cggaccttcc gcgtagccga ggtcgggggc cggggccgtc gtgccgatca
     3001 gccgccggat gtcctgggtg tgcaagtggt cgaacgcgag gtagtcgacg tccgcaggcg
     3061 agatcccgag ctggtcgagg tgctcgtcga cgtgagcgtg ccgcacgaag aacgtgtcga
     3121 gagcgcgctc gctcgtcacg ctcccgtacc cgaagcggtt cgccctgtgg gaggcctgca
     3181 acgtccccgt cccctacgtg tggatgacga accgcatgtt cgtcgtccag tggctggcag
     3241 agggccggac gaagacgctg ct
//
LOCUS       DASQDO010000248         5687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_942376, whole genome shotgun sequence.
ACCESSION   DASQDO010000248 DASQDO010000000
VERSION     DASQDO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942376"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="dxr"
                     /locus_tag="VEV43_07100"
     CDS             <1..915
                     /gene="dxr"
                     /locus_tag="VEV43_07100"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HYP23321.1"
                     /translation="AVAAELATLDEADVVLNAIVGCAGLDASVAALRSGKVLALANKE
                     SLVAGGRACLDAAAEGGGSLVPVDSEHAALAQCLARADRAEVARIVLTASGGPFRVRR
                     DLSSVTKDEALAHPTWSMGPKITVDSATLMNKGLEVIEAHWLFGFDYDDLGVLVHPQS
                     IVHGMVELRDSTVLLQAAPTDMRIPIQAALLGDERIAGGYERLDLAKVGTLEFEEVDH
                     DRFPAVGLAYRAGRAGGTAPAVLNAANEVAVGAFLEGTLRFTAIAEVVARVLDRHEPG
                     DDSTVEQVVAADRWARQEASETIAGVAA"
     gene            912..2105
                     /locus_tag="VEV43_07105"
     CDS             912..2105
                     /locus_tag="VEV43_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease"
                     /protein_id="HYP23322.1"
                     /translation="MNAGVLIVIVAILVVIMIHEAGHFFAAKAFGFKATKFFLGFGPR
                     IWSFKRGETEYGIAAIPAGGYVKIVGMNPYEEVPPEDQPRAYPNKPKWQRALVVLAGP
                     ATHWVVALVLLTVATMTIGIQTDEPTNQVNAISVRTEGSTERTAAALAGIKPDDRIVA
                     IDGEPTPEWDQISGYIRSHPDERATLTIERDGTETTVDVHFGKAIFNEEGDVVAYAGP
                     GDEIREPQPGEELTGFLGVEPATRFDKKGLGGAIGSAGSQVWDATTSSFEGIGTIFGT
                     VFNGDLLREVTGSGERTIDEESAVGIVGFVRIAGDIFALGRLYDLILLIAQFTILVGL
                     MNLLPLPPLDGGHLAVVGYEAITGKTVDIRKMIPVAAAVIAFFLVLFFAVLYLDIFRP
                     LSTGT"
     gene            2108..3277
                     /gene="ispG"
                     /locus_tag="VEV43_07110"
                     /gene_synonym="gcpE"
     CDS             2108..3277
                     /gene="ispG"
                     /locus_tag="VEV43_07110"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYP23323.1"
                     /translation="MYERRKSRQIHVGRVAVGGDAPVSIQSMTTTKTDDVNATLIQVH
                     ELATQGADIVRVAVPHEGDARALPMIVKEGGVPIVADIHFSVRLALMALEAGVHGLRL
                     NPGNMRNPKHVEEVALAAKERGIPIRIGVNAGSLDKRLVEKYGRPTAEALVESALYEI
                     GLLEQHGFTDIKVSVKHQNVAEMIQAYRLLAAQTDVPLHLGVTEAGTPKQGIVKSAIG
                     IGTLLAEGIGDTIRVSLTADPVEEVKVGKQILQSLGIRQDSFDFVACPSCWRAELDVF
                     ALANEVEAAIEKMDLPPMQIAVMGCEVNGPGEAKDADIGIAAGPGRGLLFSKGKQIGW
                     VPHDKLVEALLEETRRVADEIRAAGATGEGEAVVLKGAARRAGAPVIPIGMGPPR"
     gene            complement(3335..3487)
                     /locus_tag="VEV43_07115"
     CDS             complement(3335..3487)
                     /locus_tag="VEV43_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23324.1"
                     /translation="MRELFVEELAGVRGGATSAMDTTTACCEEGPLGCCDRYDWIFEA
                     VEDLLP"
     gene            complement(3552..3758)
                     /locus_tag="VEV43_07120"
     CDS             complement(3552..3758)
                     /locus_tag="VEV43_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23325.1"
                     /translation="MNKLVFAPLYCPPSRGKPRSAAVFDFTIGAFFTIERNTEASMAK
                     KGRKRRTRRKNKANHGKRPNAGRK"
     gene            3810..4301
                     /gene="rimP"
                     /locus_tag="VEV43_07125"
     CDS             3810..4301
                     /gene="rimP"
                     /locus_tag="VEV43_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HYP23326.1"
                     /translation="MGALEEVRDLAEAVARRRSLRLWDVEVGGQPGRAIVRVFVDGDD
                     GVDLDTVAQVSEEISRGLDLRDPIPGRYTLEVSSPGLERSLKEPAHWSASVGKKVIVK
                     TKDKLVGGSHRLDATVEGTTDGAVVLRPDGAEGAVEVAFDAIRSARTVFEWDTADKAK
                     KGG"
     gene            4307..5386
                     /gene="nusA"
                     /locus_tag="VEV43_07130"
     CDS             4307..5386
                     /gene="nusA"
                     /locus_tag="VEV43_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYP23327.1"
                     /translation="MQIPMDSLRELERERGIAFDTLVEAIEKALASAYLKVINADETR
                     GARAIVDPQTGDVTVFAQDIEIDPDTEEVTVLKEWEDTPADFGRIAAQTAKQVILQRL
                     REAERDITFGEYAGREGDIVTGIVQQQDHRYTILDLGKTEALMPPSEQIPHERYEHGS
                     RLKAYIVEVRRSARGPQIITSRTHPGLIKRLFELEVPEILDGIVEIKNVAREPGHRSK
                     IAVVSNDRNVDPVGACVGAKGSRVRMVMNELRGEKIDIIKWTDKTEEFVANALSPAKV
                     KEVRLQPDGETALVIVPDYQLSLAIGKEGQNARLAARLTGLRVDIKSESQLRKEAEEA
                     AAKQEEEAAAPAAEAEPEPAQQSED"
BASE COUNT          967 a         1857 c         2035 g          828 t
ORIGIN      
        1 gcagtcgccg cggagctcgc gaccctcgac gaggccgacg tcgtcctgaa cgcgatcgtc
       61 ggctgcgcgg ggctcgatgc ttccgtcgcc gcgctgcgct ccggcaaggt cctcgcgctc
      121 gcgaacaagg agagcctcgt cgccgggggc cgggcctgtc tcgacgcggc cgccgagggc
      181 ggaggctccc tggtgccggt cgactccgag cacgctgcgc tcgcgcagtg cctcgcgcgc
      241 gccgaccggg ccgaggtcgc gcggatcgtc ctgacggcgt cgggggggcc gttccgcgtg
      301 cggcgcgacc tctcgtcggt gacgaaggac gaggcgctcg cgcatcccac gtggtcgatg
      361 ggcccgaaga tcaccgtcga cagcgcgacg ctgatgaaca agggcctcga ggtgatcgag
      421 gcccactggc tgttcggctt cgactacgac gacctcggcg tgctcgtgca cccgcagagc
      481 atcgtccacg ggatggtcga gctgcgcgac tcgacggtgc tgctccaggc cgcgccgacc
      541 gacatgagga tcccgataca agcggccctg ctcggcgacg aacggatcgc gggcggatac
      601 gagcggctgg acctcgccaa ggtcgggacg ctcgagttcg aggaggtcga ccacgaccgg
      661 ttcccggccg tagggcttgc ctaccgggcg ggacgtgcgg gcggcaccgc ccccgccgtg
      721 ctcaacgcgg cgaacgaggt cgcggtgggc gccttcctcg aaggaacgtt gcggttcacg
      781 gcgatcgccg aggtggtcgc tcgcgtcctc gaccgccacg agcccgggga cgactccacg
      841 gtggaacaag tggtcgccgc ggatcgttgg gcaagacagg aagcttccga gacgatcgca
      901 ggagtcgctg cttgaacgcc ggcgttctca tagtcatcgt ggcgatcctc gtcgtgatca
      961 tgatccacga ggccggccac ttcttcgccg cgaaggcgtt cgggttcaag gccacgaagt
     1021 tcttcctcgg cttcggcccc cgcatctggt cgttcaagcg cggcgagacc gaatacggca
     1081 tcgcagcgat cccggcgggc ggctacgtga agatcgtcgg gatgaacccg tacgaggagg
     1141 tccctcccga agaccagccc cgtgcctacc cgaacaagcc gaagtggcag cgcgctctcg
     1201 tcgtgctcgc cgggccggcg acgcactggg tggtcgccct cgtccttctt acggtggcga
     1261 cgatgacgat cggcatccag acggacgagc cgaccaacca ggtcaacgcg atctcggtgc
     1321 gcaccgaggg gagcaccgaa cggaccgccg cggccctcgc gggcatcaag ccggacgacc
     1381 gcatcgtagc gatcgacggc gagccgacgc ccgagtggga tcagatctcc ggctacatcc
     1441 ggtcgcaccc cgacgagcgg gcgacgctga cgatcgagcg cgacgggacc gagacgaccg
     1501 tcgacgtcca cttcggcaag gcgatcttca acgaggaagg cgacgtcgtc gcgtacgccg
     1561 ggccgggcga cgagatccgt gagccccaac ccggcgagga gctgacgggg tttctcggcg
     1621 tcgagcccgc gacgaggttc gacaagaagg ggctcggcgg cgcgatcggc tcggccggat
     1681 ctcaggtgtg ggacgcgacc acgagctcgt tcgagggcat cgggacgatc ttcgggacgg
     1741 tcttcaacgg cgacctcctt agggaggtga ccggctcggg cgagcggacg atcgacgagg
     1801 agagcgcggt aggcatcgtc ggcttcgtgc ggatcgccgg cgacatcttc gccctcggcc
     1861 ggctctacga cctgatcctt ctgatcgccc agttcacgat cctggtcggg ctgatgaacc
     1921 tgctgccgct gccgccgctc gacggcgggc acctcgcggt cgtcggctac gaggcgatca
     1981 cgggcaagac cgtcgacatc cgcaagatga tcccggtcgc ggccgcggtg atcgcgttct
     2041 tcctcgtgct gttcttcgcg gtgctgtacc tcgacatctt ccggccgctg tcgacgggga
     2101 cctagcgatg tacgagcgga ggaagtcccg gcagatccac gtgggccgcg tcgcggtcgg
     2161 gggcgacgcg ccggtctcga tccagtcgat gacgacgacg aagaccgacg acgtcaacgc
     2221 gacgctgatc caggtgcacg agctcgccac gcagggagcg gacatcgttc gcgtcgcggt
     2281 gccgcacgaa ggcgacgcac gcgcgctgcc gatgatcgtc aaggagggcg gtgtcccgat
     2341 cgtcgcggac atccacttct ccgtacggct cgcgctgatg gcgctcgagg ccggcgtcca
     2401 cgggctgagg ctcaaccccg ggaacatgcg caacccgaag cacgtagagg aggtcgccct
     2461 cgcggcgaag gagcgcggta tcccgatacg catcggcgtg aacgcgggct cgctcgacaa
     2521 gcggctcgtc gagaagtacg ggaggccgac ggcggaggcc ctggtcgaga gcgcgctcta
     2581 cgagatcggg ctgctcgagc agcacgggtt caccgacatc aaggtctcgg tcaagcacca
     2641 gaacgtcgcc gagatgatcc aggcgtaccg gctgctcgcc gcgcagaccg acgtgccgct
     2701 gcacctcggc gtgaccgagg ccggcacgcc caagcagggg atcgtgaagt cggcgatcgg
     2761 gatcgggacg ctattggccg agggcatcgg cgacacgatc cgtgtctcgc tgacggccga
     2821 cccggtcgag gaggtcaagg tcggcaagca gatcctccag tccctcggga tccggcagga
     2881 ctcgttcgac ttcgtcgcct gtccgtcgtg ctggcgtgcc gagctcgacg tcttcgcgct
     2941 cgcgaacgag gtcgaggccg ccatcgagaa gatggacctg cccccgatgc agatcgcagt
     3001 catgggctgc gaggtgaacg gccccggcga ggcgaaggac gccgacatcg gcatcgcggc
     3061 agggccgggc cgcggtctgc tgttctcgaa gggcaagcag atcggatggg tcccgcacga
     3121 caagctcgtc gaggccctgc tcgaggagac gcgccgggta gccgacgaga tccgagcggc
     3181 cggcgcgacg ggcgaaggcg aagccgtggt cctgaagggg gccgcgcgcc gtgccggtgc
     3241 gccggtcatc ccgataggca tgggaccgcc ccgctagtcg gacaaagaaa aacgccgccc
     3301 cgtttcgggg cggcgtttgc agtcttacgc ggtgctacgg aaggaggtct tcgaccgcct
     3361 cgaagatcca gtcgtagcgg tcgcagcagc ccagcgggcc ttcttcgcaa caggccgtcg
     3421 tcgtgtccat cgcgctggtc gcaccgccgc gtacgcctgc gagctcctct acgaacaact
     3481 cacgcatcgt cgccaccttc cttcttgacc gggttccccg gggcttttct gcgcagccgg
     3541 tggcgattcc tctatttgcg gccggcgttg ggcctcttgc cgtggttcgc cttgttcttg
     3601 cgccgggtgc ggcgcttgcg ccccttctta gccatgctcg cctccgtgtt ccgttcgatt
     3661 gtgaaaaacg cgcctatggt aaaatcgaaa accgccgctg acctgggctt tcccctggac
     3721 ggcgggcagt atagaggcgc gaagactagc ttgttcaagg gcccctccca cgaggggctt
     3781 tgtcgtgggg gcctggaaaa ggaggcgttg tgggtgcgct cgaagaggtc agggacctcg
     3841 ccgaggcggt ggcccgacgc cggtcgctgc gcctctggga cgtcgaagta ggcggacagc
     3901 cgggccgcgc gatcgtccgg gtcttcgtcg acggcgacga cggggtcgac ctggacacgg
     3961 tcgcgcaggt ctcggaggaa atctcccggg ggctcgacct gagggacccg atccccggcc
     4021 gctacacgct ggaggtctct tcgcccggcc tcgagaggtc gctgaaggag ccggcccact
     4081 ggagcgcctc ggtcggcaag aaagtgatcg tgaagacgaa ggacaagctg gtggggggca
     4141 gtcaccggct cgacgcgacg gtggaaggca ccacggacgg cgcggtcgtc ctgcgccccg
     4201 acggggccga aggggcggtc gaggtggcgt tcgacgccat ccgctcggcc cgcacggtgt
     4261 tcgagtggga caccgccgac aaggctaaga agggaggctg aggcacatgc agataccgat
     4321 ggattcgctg cgcgagctgg aacgcgagcg cggcatcgcg ttcgacacgc tggtcgaagc
     4381 gatcgagaag gccttggcgt ccgcatacct gaaggtcatc aacgccgacg agacgcgcgg
     4441 tgcccgggcg atcgtcgacc ctcagaccgg cgacgtcacg gtgttcgcgc aggacatcga
     4501 gatcgacccc gacaccgagg aggtgacggt cctcaaggag tgggaggaca cgcccgccga
     4561 cttcggccgc atcgcggcgc agactgcgaa gcaggtgatc ctccaaaggc tgcgcgaggc
     4621 cgagcgcgac atcacgttcg gcgagtacgc cggccgcgag ggcgacatcg tcactgggat
     4681 cgtccagcag caggaccacc gctacacgat cctcgacctc ggcaagaccg aggccctcat
     4741 gccgccgtcg gagcagatcc cgcacgagcg ctacgagcac ggctcgcgcc tcaaggccta
     4801 catcgtcgag gtacgccggt cggcgcgcgg cccccagatc atcacgtcgc gcacgcaccc
     4861 ggggctcatc aagcgtctgt tcgagctcga ggtgcccgag atcctcgacg gcatcgtcga
     4921 gatcaagaac gtggcgcgcg agccgggcca ccgctcgaag atcgccgtgg tctccaacga
     4981 ccgcaacgtc gaccccgtcg gcgcctgcgt cggcgcgaag gggtcgaggg tgcgcatggt
     5041 catgaacgag ctccgcggcg agaagatcga catcatcaag tggaccgaca agaccgagga
     5101 gttcgtcgcg aacgcgctgt cgccggcgaa ggtcaaggag gtccggctcc agcccgacgg
     5161 cgagaccgcc ctggtgatcg tcccggacta ccagctctcg ctggcgatcg ggaaggaggg
     5221 ccagaacgcc cggctcgcgg cgaggctcac ggggctgagg gtggacatca agtcggagtc
     5281 gcagctccgc aaggaggccg aggaggccgc tgccaaacag gaggaagaag cggcggcgcc
     5341 ggcagccgag gcggagcccg agcccgctca gcagagcgaa gactaagatg gcgtctcccg
     5401 tgcgaacctg cgcggggtgc cgagcccgcc ggccgaagga ggacctgacc agggtcgtgc
     5461 gggggccgga cgggatcgtg cgtctgtcga cgtccgggct cggcggtagg ggcgcctaca
     5521 cgtgccccga cgagaggtgc atacggcagg cgttcgagac cggacgactc cgccgggtgc
     5581 tccggattga aggagatgga cttcccggct cactgccggg cgagatgctt gaagaagtga
     5641 aggggcgcgt atggcaaagc cgagggttca cgaagtagcg aaagagc
//
LOCUS       DASQDO010000249         5646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_942562, whole genome shotgun sequence.
ACCESSION   DASQDO010000249 DASQDO010000000
VERSION     DASQDO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942562"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VEV43_07135"
     CDS             <1..284
                     /locus_tag="VEV43_07135"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYP23328.1"
                     /translation="AHQANGRILAAVADRLGLPPGKMFTNVARYGNTTAGSIPIALAE
                     AHAQSALSDGDLVVLAAFGSGFVWGGGLVRWGVPAPVEDELAGAGAARV"
     gene            277..1008
                     /gene="fabG"
                     /locus_tag="VEV43_07140"
     CDS             277..1008
                     /gene="fabG"
                     /locus_tag="VEV43_07140"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HYP23329.1"
                     /translation="MSSVALVTGASRGIGRAIAVALAPGRAVAVNYRANAEEAKETLR
                     LVEAAGGEGIVVEGDVRDAASVERLFGEVEDALGAVSVLVANAGVRADGLAARLSDDA
                     WADVVDTNLYGTFACARRALRPMLRAGWGRIVNVASVAGLAGNAGQANYCAAKAGVVG
                     LTRSLAREVARKGITVNAVAPGLIDTDLVASLDDQQRGDLLGSIPAGRSGTPEEVAAA
                     VAFLCSEQAAYVTGTVLTIDGGMTA"
     gene            1031..1279
                     /locus_tag="VEV43_07145"
     CDS             1031..1279
                     /locus_tag="VEV43_07145"
                     /inference="COORDINATES: protein motif:HMM:NF002148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYP23330.1"
                     /translation="MTQTDVLEAIREHLGGRGLDTSKVVPEADLLKDLDLDSLDTMEL
                     TLGLEERFGIEIPDQELEGLATVADAVGLVERKLSVGA"
     gene            1276..2505
                     /gene="fabF"
                     /locus_tag="VEV43_07150"
     CDS             1276..2505
                     /gene="fabF"
                     /locus_tag="VEV43_07150"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYP23331.1"
                     /translation="MTHAVVTGIGAVTPIGTGAEDFWAALVAGRSGAAPIEGFDASPY
                     STRIACQVRGFDPADHMDGRTAARADRFTQLAIAAGGQAWADAGLGDDIDRDRAGVIV
                     GSGIGGLYTIESQSAALIDGGVRRVSPFTVPKLMPNAAAAAIAMQLGLRGINYCVTSA
                     CATGAHAIGEALLAIRSGRADVVLAGGTEAALTPLGLAAFDRMGALSRRNDEPERASR
                     PFDAGRDGFVFGEGAAVLVLESDDHARRRGARAIARVAGYGATADAFHLTQPDPEGDG
                     ATRAMTAALEDARVYPQDVDYVNAHGTSTPYNDRVETIAIKRALGPESKRIPVTSTKS
                     QTGHLLGAAGAVEAAATALIVDRGLVPRTLNRDVPDPECDLDYVPDEPREQRVSVALS
                     NSFGFGGQNACLVLTAP"
     gene            2502..3260
                     /locus_tag="VEV43_07155"
     CDS             2502..3260
                     /locus_tag="VEV43_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP23332.1"
                     /translation="MSERDLDGRVALVTGGGRGIGRAIATSLAARGANLVVAYLRNRA
                     AAEETCAAAEDHAVKALAVRANVGDEKHLDALFDRVAETFGRLDVFVSNAATGVIKGL
                     DELDARAWAWTMDANARAFFLGAKRAAPLMAEGGSIVALSSQGSTRVLPGYMVVGASK
                     AALEAVARYLAVELGPRGIRVNVVSPGVVDTDALKHFPAREEMLEAARSKTPLGRLSA
                     PADVAEVVGFLTSERAAMITAQTLVVDGGAGVLA"
     gene            3257..3607
                     /locus_tag="VEV43_07160"
     CDS             3257..3607
                     /locus_tag="VEV43_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HYP23333.1"
                     /translation="MSLGVDVVEIERFRGVLDRNPGFLARFFTNEERAHCGTSLAGLA
                     GTFAAKEAVMKAAGLTPAPAWARRIRVCRDASGKPVATVAGRTIAVSISHDGPVAVAV
                     ALDAQEAGAATPAS"
     gene            complement(3618..4319)
                     /locus_tag="VEV43_07165"
     CDS             complement(3618..4319)
                     /locus_tag="VEV43_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23334.1"
                     /translation="MRKHMRVAAVVAGAALLVGATIGGSAQAAKKKKGPVVVGTDAAD
                     DWGSNVDATIAPVGNQLGQELVEASIEKVDAATLSFNIKVAGLPPSGGVPELTRYVWT
                     MTVDGTLLQLDGKFTNYSRGACDPTAGTCPPPRDPGQAPFMVRGNCTDNGGTAVTCEE
                     IGLVNGTFDAASGTITIPVPLELLGAKPGSVIAHGMQAGSNFAGVWAIPSAWASQGQM
                     PLDEMIITKPYKVTR"
     gene            4446..4634
                     /locus_tag="VEV43_07170"
     CDS             4446..4634
                     /locus_tag="VEV43_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23335.1"
                     /translation="MRKTRMLLAATILAVPMGLVTASPAMACKQQPCPPACKLVSPVQ
                     VSGTDVVFTGRGPIECYY"
     gene            complement(4690..5433)
                     /locus_tag="VEV43_07175"
     CDS             complement(4690..5433)
                     /locus_tag="VEV43_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP23336.1"
                     /translation="MEIVRDAAPGIHQVTDAYTNWFLVEDGGRVTIVDAGVPASWRSL
                     HAAAPELGLNVSDVEAIVLTHAHFDHLGFAERARRELGVDVWVHANDAPLTRHPLQYT
                     HERARSYYFVTKPRALPIVAALTARRAWWPRAVQEVRRYEGGTLDVPGKPSVVPTPGH
                     TVGHCALYFPERDCVIAGDAVVTLDPYTGSTGPRLVARGATADSTRALQSLDRLAETG
                     AGTVLVGHGPAWTAGAADAVERARAAGVA"
BASE COUNT          779 a         1977 c         2100 g          790 t
ORIGIN      
        1 tcgcgcacca ggccaacggg aggatcctcg ccgcggtcgc cgaccgcctc gggcttccgc
       61 ccggcaagat gttcacgaac gtggcccgtt acggcaacac gacggcgggc tcgatcccga
      121 tagcgctcgc cgaagcgcac gcgcagagcg ctctcagcga cggcgacctc gtcgtgctcg
      181 ccgcgttcgg gtccgggttc gtgtggggag ggggcctcgt gcgctggggt gtcccggccc
      241 ccgtcgaaga cgagctcgcg ggagcggggg ccgctcgtgt ctagcgtcgc cctcgtcacg
      301 ggtgcctcgc gcgggatcgg gcgggcgatc gcggtcgcgc tggcgccggg acgtgccgtc
      361 gccgtgaact accgggcgaa cgccgaggag gccaaggaga cgctgcgcct cgtcgaggcc
      421 gcgggcggcg aagggatcgt cgtcgagggc gacgtgcgcg acgccgcctc ggtcgagagg
      481 ctgttcggcg aggtggagga cgcactcggg gccgtctccg tcctcgtcgc caacgcgggg
      541 gttcgcgccg acggcctggc cgcacggctg agcgacgacg cgtgggcgga cgtggtggac
      601 accaacctct acgggacgtt cgcctgcgcg cggcgcgcgc ttcgtccgat gctgcgcgcc
      661 gggtggggac gcatcgtcaa cgtcgcgtcg gtggccggac tggccggcaa cgcgggacag
      721 gcgaactact gcgccgcgaa ggccggagtc gtcggcttga cgcgctcgct ggcgcgggag
      781 gtcgcgcgca agggcatcac ggtgaacgcg gtcgcgccgg ggctgatcga cacggacctc
      841 gtcgcgagcc tcgacgatca gcagagaggc gacctgctcg gctcgatccc ggcgggccgc
      901 tcggggactc cggaggaggt agcggccgcg gtcgcgttcc tgtgctcgga gcaggccgcg
      961 tacgtgactg gaacggtcct cacgatcgac ggggggatga cggcatgacg accaggaagg
     1021 agatcgggag atgacacaga ccgacgtact ggaggcgata cgcgagcacc tcgggggccg
     1081 cgggctcgac acgagcaagg tggtgcccga ggcggacctg ctgaaggacc tggacctcga
     1141 ctcgctcgac acgatggagc tgacgctcgg gctcgaggag cggttcggca tcgagatccc
     1201 cgaccaggag ctcgagggcc tcgcgacggt cgcggacgcc gtggggctgg tggagcgcaa
     1261 gctgtcggtg ggcgcgtgac gcacgccgtc gtaaccggca tcggcgccgt cacgccgatc
     1321 ggcacggggg ccgaggactt ctgggccgcg ctggtcgccg ggcgttcggg ggccgctcct
     1381 atcgagggct tcgacgcgtc gccgtactcg actcggatcg cgtgtcaggt ccgcggcttc
     1441 gacccggccg accacatgga cgggcgtacc gcagcgcggg ccgaccggtt cacgcagctc
     1501 gcgatcgcgg cggggggcca ggcgtgggcg gatgccggtc tcggggacga catcgaccgc
     1561 gaccgcgccg gcgtgatcgt cggctcgggc atcggcggcc tctacacgat cgagtcacag
     1621 tccgccgcgc tcatagatgg cggcgtccgg cgcgtgagcc cgttcacggt gcccaagctg
     1681 atgccgaacg cggccgcggc ggcgatcgcc atgcagctcg gactgcgcgg gatcaactat
     1741 tgcgtgacct ctgcctgcgc gacgggagcg cacgccatag gcgaggcgct tcttgcgatc
     1801 cggtcgggcc gcgcggacgt cgtgctcgcc ggcgggacgg aggccgcgct gacgcctctg
     1861 ggcctcgccg cgttcgaccg gatgggggcg ctgtcgcgcc gcaacgacga gcccgagcgt
     1921 gcgagccgtc ccttcgacgc gggccgtgac ggcttcgtgt tcggggaagg ggcggccgtc
     1981 ctggtgctcg agagcgacga ccatgcacgg cggcgggggg cgcgggcgat cgcacgggtg
     2041 gccggctacg gcgcgaccgc cgacgccttc cacctcacgc agcccgatcc cgaaggcgac
     2101 ggcgcgaccc gggcgatgac cgccgcgctc gaggacgccc gcgtgtatcc gcaggacgtc
     2161 gactacgtga acgcgcacgg cacctcgacg ccgtacaacg atcgcgtcga gacgatcgcg
     2221 atcaagcggg cgctcggccc ggagtccaag cgcatcccgg tgacctcgac taagtcgcag
     2281 accggccacc tgctcggcgc ggcgggcgcg gtcgaggccg ccgctaccgc cttgatcgtc
     2341 gaccgcgggc tcgtgccgcg cacgctcaac cgcgacgtgc ccgaccccga gtgcgacctc
     2401 gactacgtcc ccgacgagcc gcgcgagcag cgcgtgtcgg tcgcgctgtc gaactcgttc
     2461 ggcttcggcg ggcagaacgc atgcctcgtc ctgacggcgc catgagcgag cgcgacctgg
     2521 acgggcgcgt cgccctcgtc acgggtgggg gccgcgggat cggccgcgcg atagcgacga
     2581 gcctcgccgc gcgcggagcg aacctcgtcg tcgcgtacct gcgcaaccgc gccgccgccg
     2641 aagagacgtg cgcggcggcc gaggaccacg ctgtcaaggc cctggcggtg cgagccaacg
     2701 tgggcgacga gaagcatctc gacgctctgt tcgaccgcgt cgccgagacc ttcgggcggc
     2761 tcgacgtctt cgtctcgaac gctgcaacgg gcgtgatcaa gggactggac gagctcgacg
     2821 cgcgcgcctg ggcgtggacg atggacgcca acgcccgggc attcttcctc ggcgcgaagc
     2881 gcgcggcccc cctgatggcc gaaggaggct cgatcgtcgc gctgtcgagc cagggctcga
     2941 cgagggtgct cccgggctac atggtcgtcg gcgcctcgaa ggccgccctc gaagccgtcg
     3001 ctcgctacct cgcggtcgag ctcggccccc gcggcatccg cgtcaacgtc gtctcccccg
     3061 gcgtcgtcga caccgacgcg ttgaagcact tccccgcccg cgaggagatg ctcgaggcgg
     3121 cacggtccaa gaccccgctc gggcgcctga gcgcgccggc ggacgtagcc gaggtcgtgg
     3181 gcttcctcac ctcggagcgg gccgcgatga tcaccgcgca gacgctcgtc gtcgacggcg
     3241 gtgccggagt gctcgcatga gcctcggcgt agacgtcgta gagatcgagc gcttccgcgg
     3301 cgtcctcgac cgcaaccccg gcttcctggc gcggttcttc acgaacgagg agcgcgcgca
     3361 ttgcgggacg tcgctcgccg gccttgccgg gacgttcgct gcgaaggaag cggtgatgaa
     3421 ggcggccggg ctcacacccg cgccggcatg ggcccgccgg atccgggtgt gccgggacgc
     3481 ttcgggaaag cccgtcgcca cggtcgccgg gcggacgatc gcggtgtcga tctcgcacga
     3541 cggaccggtc gccgtcgcgg tcgcgctgga cgcgcaagag gcgggggccg caacccccgc
     3601 ctcctagttg ctgcgagcta ccgcgtgacc ttgtaaggct tcgtgatgat catctcgtcg
     3661 agcggcatct ggccctgcga ggcccacgcc gacgggatcg cccacacgcc ggcgaagttt
     3721 gagccggcct gcatgccgtg agcgatgacg ctgcccggct tggcgccgag gagctcgagc
     3781 gggaccggga tcgtgatggt gccgctcgcc gcgtcgaagg tcccgttgac gaggccgatc
     3841 tcctcgcacg tcacggccgt gccgccgttg tcggtgcagt tgccgcggac catgaacggg
     3901 gcctggcccg ggtcgcgcgg aggcgggcac gtcccggcgg tcggatcgca cgcgccgcgg
     3961 gagtagttcg tgaacttgcc gtccagctgg agcagggtcc cgtccacggt catcgtccag
     4021 acgtagcggg tgagctcggg cacgccaccc gacggaggca gcccggcgac ctttatgttg
     4081 aagctcagcg tcgcggcgtc gaccttctcg atcgaggcct cgacgagctc ctgaccgagc
     4141 tggttcccta ccggcgcgat cgtcgcgtcg acgttcgagc cccagtcgtc cgccgcgtcc
     4201 gtgccgacca cgaccgggcc cttcttcttc ttcgccgcct gggcggagcc gccgatcgtc
     4261 gccccgacga gcagggcagc gccggccacg acggccgcca ctcgcatgtg ctttctcatg
     4321 tcgtctcccc agtgagttgt cggttgatct cctccctcag ggttcgccgg ggggacgggg
     4381 catccttcac ctttgcccgc tatgctcccc ggccgaaagc gaacatttcg gacaaggggg
     4441 atgagatgcg caagacgagg atgctcctcg cagcgacgat cctggccgtg cccatggggc
     4501 tcgtgacggc ctcgcctgcg atggcgtgca agcagcagcc gtgcccgccg gcctgcaagc
     4561 tggtctcgcc cgtgcaggtg agcgggaccg acgtggtgtt caccgggcgc ggcccgatcg
     4621 agtgctacta ctagaccgac cgtcgaagtc gaaggccccc ggttcgccgg gggccttttc
     4681 ctttgcgcgt caggcgaccc cggccgccct cgctctctcg acggcgtccg cggccccggc
     4741 ggtccaggcg gggccgtgcc cgacgagcac ggtccccgct ccggtctccg ctagccggtc
     4801 cagcgactgc agcgcgcgcg tcgagtccgc ggtggcgccg cgggcgacca ggcgggggcc
     4861 ggtcgatccc gtgtacgggt cgagcgtgac gaccgcgtcg ccggcgatca cgcagtccct
     4921 ctccgggaag tacagcgcgc agtgcccgac ggtgtggccg ggcgtcggga cgaccgacgg
     4981 cttgccgggg acgtcgaggg tcccgccttc gtagcggcgg acctcctgca cggcccgcgg
     5041 ccaccacgcg cgtctcgccg tcagcgccgc cacgatgggc aacgcccgcg gcttcgtcac
     5101 gaagtaatag gagcgggcgc gctcgtgcgt gtactgcaac gggtgccggg tcagcggcgc
     5161 gtcgttcgcg tgcacccaga cgtcgacgcc gagctcgcgc cgggctctct ccgcgaagcc
     5221 gaggtggtcg aagtgcgcgt gcgtgaggac gatcgcctcg acgtccgaga cgttcaggcc
     5281 gagctcgggg gccgcggcgt gcagcgagcg ccacgacgcc ggaacgcccg cgtcgacgat
     5341 cgtgacgcgg cccccgtcct cgacgaggaa ccagttcgtg tacgcgtcgg tgacctgatg
     5401 gatccccggg gcggcgtccc tgacgatctc catccaccgg cgatacccgg atggcggttt
     5461 ccgaagcccg gtcgcgggga tatcgccgcg acgaccactt gctcgaggga ggtcccccat
     5521 ggagcgcgag aacgaagaag cccagtccgg ccccgtgacg cacggcggcg cgggtgatcc
     5581 tctgggcgaa ggcgtcgccg agccgggcga gaacgacgcc tcgacggccg acgagacgga
     5641 ccagaa
//
LOCUS       DASQDO010000250         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_942634, whole genome shotgun sequence.
ACCESSION   DASQDO010000250 DASQDO010000000
VERSION     DASQDO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942634"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VEV43_07180"
     CDS             complement(<1..1494)
                     /locus_tag="VEV43_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP23337.1"
                     /translation="MHFRRGVDTSRIRETLRQHGAGLEERDGSSVLVETNGRPVRQLL
                     SDLEENPLVADAEPNRIFTVAGAPNDPLYEHQRPYLETAGLPDAWATTKGTTGTTVAI
                     LDTGIDLDHPEFAGRILPGRDFVNNDADPTDDEGHGTMVAGVAAAAADNGIGIAGAAG
                     GVSILPVKVLDRNGQGDTWQISEGIKWAVAQGADVLNFSFTGGGSDRFDDAVRDAVRR
                     NVVIVAAAGNDGEAWPYLPRSYENVLTVAATDHAGTFAWFSNHGPLVELAAPGVDITT
                     TARAPGAQDAYAVASGTSFSTPLVSGAAALVRSVHPDWTVDQVARRLERTARDAGPPG
                     VDDSYGFGIVDAASALADRRRTALPSVTGAEEPNNVLARATTFTGSAGGTISPEGDVD
                     WFATTLAAPGWVTVRLTGPAVDPEGRRPEELDPVLKVYDPAGDPLRTSDGGSIGDPEG
                     VLVPVATSGRYLIRVENYVGSRSPGPYTIEVAPAAAPARFAPATEQTL"
BASE COUNT          206 a          561 c          547 g          246 t
ORIGIN      
        1 tagggtctgc tccgtcgccg gcgcgaatcg cgccggcgca gcggcgggcg cgacctcgat
       61 cgtgtacggc cccggactgc gcgagccgac gtagttctcg acgcggatca ggtacctacc
      121 gcttgtcgcg accgggacga gcactccctc gggatccccg atcgagcctc cgtcactcgt
      181 ccgcaaggga tcgccggcgg ggtcgtacac cttcagcacc ggatcgagct cctcgggccg
      241 tcgcccctcg gggtcaacag cgggccccgt caatcgcaca gtgacccacc cgggcgccgc
      301 gagcgtcgtc gcgaaccagt cgacgtctcc ctcgggcgag atcgtgcccc ctgcggaacc
      361 ggtgaacgtc gtcgcgcgtg cgaggacgtt gttcggctcc tcggcgcctg taacggacgg
      421 aagcgcggtg cggcgcctgt ccgccagcgc ggaggctgcg tcgacgatcc cgaacccgta
      481 ggaatcgtcg acgcccggtg gacctgcgtc ccgcgccgtc ctctcgagcc gccgggccac
      541 ctggtcgacc gtccaatcgg gatggacaga cctgacgagc gcggcggcgc ccgagacgag
      601 aggcgtcgag aaggacgtcc cgctcgcgac ggcgtacgca tcctgcgcac ccggcgcgcg
      661 agcggtcgtc gtgatgtcga cgccgggcgc cgcgagctcg acgagggggc cgtggttcga
      721 aaaccaggcg aacgtgccgg cgtgatccgt tgccgcgacc gtgagcacgt tctcgtacga
      781 gcggggcagg tagggccagg cctcgccgtc gttgccggcc gccgcgacga tcacgacgtt
      841 ccgacgcacg gcgtctcgca ccgcgtcgtc gaacctgtcg ctgccgccgc ccgtgaacga
      901 aaagttgagg acgtccgcgc cctgtgcgac cgcccacttg attccctcgc tgatctgcca
      961 ggtgtccccc tgcccgttcc tgtcgaggac cttgaccggc aggatcgaga cgccgccagc
     1021 cgcgcccgcg atgccgattc cgttgtcggc cgccgcggcc gcgacgcccg cgaccatcgt
     1081 tccgtggcct tcgtcgtctg tggggtccgc gtcgttgttg acgaaatcgc gtccgggcag
     1141 gatgcgcccg gcgaactcgg ggtggtcgag atcgatgccg gtgtcgagga tcgcgaccgt
     1201 cgtcccggtc gtccccttcg tggtcgccca cgcgtcgggt agccccgccg tctcgaggta
     1261 cggccgctgg tgttcgtaga ggggatcgtt cggggcgccc gcgaccgtga agatccggtt
     1321 cggctcggcg tcggcgacga ggggattctc ctccaggtcg ctcagcagtt ggcgcacggg
     1381 acgtccgttc gtctccacga ggacggagct cccgtcgcgc tcctcgagac ccgcgccgtg
     1441 ctgccgcagc gtctcgcgga tccgcgacgt gtcgacgccc ctgcggaagt gcacgatcac
     1501 cgtgctgcga tcgatctccg acgtctgctg cttcgagggg acgggcgcgg gcagccggac
//
LOCUS       DASQDO010000251         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_270233, whole genome shotgun sequence.
ACCESSION   DASQDO010000251 DASQDO010000000
VERSION     DASQDO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270233"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VEV43_07185"
     CDS             complement(<1..1283)
                     /locus_tag="VEV43_07185"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23338.1"
                     /translation="MSGFLQSPPRLPNAWSSDATLREALRWRLGDDLFEAAEPELDSM
                     GAAAVDDATIELALRAEQEPPIHVPYTAWGERVDEIRVSDAYLALGKLGVRAGVTALP
                     YEDGPYGPAARVVWGALIALWGPSSALYSCPIAMTDAAARTLLVHGGPSDVPVAERLV
                     SRDPERAWTSGQWMTETTGGSDVGGTETVARRDGDAWRLYGTKWFTSATTSEIALTLA
                     RPEGAPEGSKGLVLFRVHRRTDDGRPNGIVVRRLKDKLGTRALPTAELELDGAVAWPV
                     GDVEGGGLRRIATMLNLTRVHNALNAAGALARGLAWARAFAEVRQVGGRRLISLPAHR
                     ATLADLAVDHAAALALVLKCCELTGRAEHGRATPDEERMLRGLTPVTKLATARWAVAG
                     VAEAMECLGGVGYCEDSGLPALVRNTHVMPIWEGTT"
     gene            1308..2237
                     /locus_tag="VEV43_07190"
     CDS             1308..2237
                     /locus_tag="VEV43_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP23339.1"
                     /translation="MPDPAIEVRNLTKSYGDVQAVKGVDLRVEQGEIFALLGPNGAGK
                     TTTVEILEGHRKRSGGEVSVLGHDPSRGERAFKERIGIVLQETGVEPYLTVAEVIDLF
                     RGYYPNPLPVDEIVELVGLGDKRDTRVSKLSGGQQRRLDVAIGLAGNPDLLFLDEPTT
                     GFDPSARRGAWKIVKDLAAGGTTVFLTTHYMDEAQFLADRVAIIVGGKIIAEGPPSTL
                     ATHDLASTAITFALPEGRSLPDSVGAATVDGELVELRTSEPMQTLHELTGWALREGVE
                     LQGITVSRPSLEDVYLDLTSDGLGRRPEQGGQP"
     gene            2234..>2509
                     /locus_tag="VEV43_07195"
     CDS             2234..>2509
                     /locus_tag="VEV43_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP23340.1"
                     /translation="MSTASLTLRQVGFENRSFWRNPPAAFFTFVFPLMFLVIFNLIFG
                     GNHIPRGGGRTSSGSTFYVPAIAAFSVIRAAYTNIAGSVATARDLGIR"
BASE COUNT          368 a          927 c          862 g          352 t
ORIGIN      
        1 gtcgtgccct cccagatcgg catgacgtgg gtgtttcgga cgagcgccgg gagccccgag
       61 tcctcgcagt agccgacgcc gccgaggcac tccatcgcct ctgccacccc tgcgacggcc
      121 cagcgagcgg tcgcgagctt ggtcaccggc gtcaacccgc ggagcatccg ctcctcgtcc
      181 ggggtcgccc gtccgtgctc cgcccgcccg gtgagctcgc agcacttcag gaccagggcc
      241 agcgccgccg catggtcgac cgcgaggtcg gccagcgtgg cccggtgcgc gggcagggag
      301 atcagacggc ggccgccgac ctgcctgacc tcggcgaagg cacgggccca ggcgagcccc
      361 cgcgcgagcg cgcccgccgc gttcagcgcg ttgtggacgc gcgtcaggtt gagcatggtc
      421 gctatccggc ggagcccgcc gccttcgacg tcgcccaccg gccaggcgac ggcgccgtcg
      481 agctcgagct cggccgtcgg caacgcccgc gtccccagct tgtccttgag gcggcgcacg
      541 acgatcccgt tcggacgccc gtcgtcggtc ctgcggtgca cgcggaacag caccagccct
      601 ttggacccct cgggcgcgcc ctcgggccgc gcgagcgtca acgcgatctc ggaggtcgtt
      661 gcgctcgtga accacttcgt gccgtagagc cgccacgcgt cgccgtcgcg ccgggcgacg
      721 gtctcggtcc cgccgacgtc ggagcctccg gtcgtctcgg tcatccactg cccgctcgtc
      781 caggcgcgct cggggtcgcg cgacaccagg cgctcggcga ccggcacgtc ggaaggcccg
      841 ccgtgcacga gcagcgtgcg cgcggctgcg tccgtcatcg cgatcggaca gctgtagagc
      901 gcggacgacg gtccccacag cgctatcaga gcgccccaca cgacccgcgc agccgggccg
      961 tacggtccgt cttcgtacgg cagcgcggtg acgccggcgc gcaccccgag cttcccgagc
     1021 gcgaggtagg cgtcggagac ccggatctcg tcgacacgct ctccccaggc tgtataggga
     1081 acgtgaatcg gtggctcctg ctcggcccgc agcgccagct cgatcgtcgc gtcgtcgacg
     1141 gcggccgctc ccatcgaatc gagctcgggc tccgccgcct cgaagaggtc gtcgccgagc
     1201 ctccagcgga gcgcctcgcg cagcgtcgcg tccgaggacc acgcgttcgg cagacggggc
     1261 ggactctgga ggaagccgct catggcccca gcttatgctc ggcgccgatg cccgacccag
     1321 ccatagaagt gcgcaacctg acgaagtcct acggcgacgt gcaagccgtg aagggcgtag
     1381 acctgcgcgt cgagcagggc gagatcttcg ccctcctggg gccgaacggg gccggcaaga
     1441 cgacgacggt cgagatcctc gaaggccacc gcaagcgctc cgggggcgag gtctccgtcc
     1501 tcggccacga cccgagccgc ggcgaacgcg ccttcaagga gcggatcggg atcgtcctcc
     1561 aggagaccgg ggtcgagccc tacctgaccg tcgccgaagt gatcgatctg ttccgcggct
     1621 actacccgaa cccgctgccc gtggacgaga tcgtcgagct cgtgggattg ggcgacaagc
     1681 gggacacccg ggtgagcaag ctctcgggcg gccagcagcg acggctcgac gtggcgatcg
     1741 gcctcgccgg caaccccgac ctgctgttcc tcgacgagcc gaccacgggc ttcgacccgt
     1801 cggcgcgcag gggcgcgtgg aagatcgtga aggacctcgc cgccggcggg acgacggtct
     1861 tcctgacgac gcactacatg gacgaggcgc agttcctcgc cgaccgcgtc gcgatcatcg
     1921 tcggcgggaa gatcatcgcg gagggcccgc cgtccaccct cgcgacccac gacctcgcgt
     1981 cgacggcgat caccttcgcc ctccccgagg gccgctcgct tccggactcg gtaggcgctg
     2041 cgacggtcga cggagagctc gtcgagctga ggacgtccga gcccatgcag acgctgcacg
     2101 agctgacggg ctgggcgctg agagaaggag tcgagctcca agggatcacg gtgtcccgcc
     2161 cgtcgctcga agacgtctac ctcgacctca cgagcgacgg gctcggccgt cgcccggagc
     2221 agggcgggca gccgtgagca ctgcatcgct caccttgcga caggtcggct tcgagaaccg
     2281 gagcttctgg cgcaacccgc ccgccgcgtt cttcacgttc gtgttcccgc tgatgttcct
     2341 cgtgatcttc aacctgatct tcggcggcaa ccacatcccg agaggagggg gccgcacgag
     2401 cagtgggtcg accttctacg tacctgccat cgcggcgttc tcggtgatcc gcgcggcgta
     2461 cacgaacatc gcggggtcgg tcgcgaccgc gcgcgacctc ggcatccgc
//
LOCUS       DASQDO010000252         6654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_209059, whole genome shotgun sequence.
ACCESSION   DASQDO010000252 DASQDO010000000
VERSION     DASQDO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209059"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VEV43_07200"
     CDS             complement(<1..819)
                     /locus_tag="VEV43_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYP23341.1"
                     /translation="MTTSWQRNGSMRSASRLLEVVQRRKQLTVKEMTEATGLSRGTVT
                     NLVDQLVNLNVLTKSAGPGDHVGRRPQVISLGRAAGVLVGVELGFYAIAIAVEPPDGP
                     MLEIRVPYDHRIVENPFSKTLGEAVDLAADALRDAGIGGTEIRGIGLAIPAPVDHTTG
                     TVLSRDALRGWPPIAPHDEFASQLASALGIRDAIPALLGNDAQLGAWGTYCSRTAPEP
                     AELVPGRGTAGRNKLMFIKASEAIRVGFVNNGAPDWGCSFPSGDFSHQAVQLGAA"
     gene            complement(2065..2964)
                     /gene="ligD"
                     /locus_tag="VEV43_07205"
     CDS             complement(2065..2964)
                     /gene="ligD"
                     /locus_tag="VEV43_07205"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYP23342.1"
                     /translation="MEILAGRRKVSVSNPDKVFYPETGFTKLDVARYYQDVAPVLLPH
                     VKNRLVTLKRYPNGVEGDFFYEKQCPKHRPDWIGTAPMVRKDGKTINYCLLNDRAALA
                     WASNLANLELHTSLARADRLDRPRALVFDLDPGEGTDLLDCARVALWIRALFSDLGLE
                     SFAKTSGSKGIQMFVPVSLSATFDDTRAWAHSVALRLESEHPDAIVTKQSKEARRGKV
                     LIDWSQNDDHKTTVAVYSLRAKTRPTVSTPVTWDEVEAALSSDDPAALVFESDQVLAR
                     IEEHGDLFEPVLKLRQKLPAPPS"
     gene            complement(2964..4037)
                     /locus_tag="VEV43_07210"
     CDS             complement(2964..4037)
                     /locus_tag="VEV43_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP23343.1"
                     /translation="MVGTHGLKVAITGATGNVGTSLIRSLQNEPAVGSILGIARREPA
                     MRVPKVTWRRADIVTDDLVRLFSGCDVVVHLAWLIQPSRDLRILHATNVDGSRRVFEA
                     AAAAGVPSLVYASSIGAYSPGPKDDSGVDESWPVEGVESSFYSRHKAATESILDEVER
                     ANPRMRVVRLRPGLIFKRESAAEVRRLFFGPLLPGTLIRRSLIPFLPDIDALRFQVVH
                     SYDVGDAYRLAIVKGVSGAFNVATQPVMDMKRLAELLGARPVKIPASVLKIGADVTWK
                     LRLQPTPSGWLDMALQVPIMDTTRARTELGWKPRHDAADAILDLMEGLREGAGMDTPP
                     LDPKAGGRGRVKELLTRVGGASR"
     gene            complement(4043..4819)
                     /locus_tag="VEV43_07215"
     CDS             complement(4043..4819)
                     /locus_tag="VEV43_07215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP23344.1"
                     /translation="MDRRIAIVTASDSGIGKATAVALARAGCDVGITWNSDEAGAKET
                     ANEVGALGRRAEVRHLDLTNLPGAADVVDELAAELGGLDVFVNNAGTGDSTPFLEQDY
                     ADWLKVLDVDLNGAFLCAQRAARIMVANKTEGRIVNVTSVHEHTPKEGSAAYCTAKGG
                     LGLLTKVMALELAEHGITVNAVAPGEIATAMTGNEDVDPAEVERPGIPMGRPGDAWEV
                     AHVIAHLASPEASYTTGASYVVDGGLMLMPAMANVIAKPE"
     gene            4891..6066
                     /gene="dinB"
                     /locus_tag="VEV43_07220"
     CDS             4891..6066
                     /gene="dinB"
                     /locus_tag="VEV43_07220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYP23345.1"
                     /translation="MKVATPILHVDMDAFYASVEVIKDPSLKGKPVIVGGQGGRGVVT
                     SASYEAREFGVRSAMPTIQARRLCPRGIFLPNDFAAYQDLSAKIREVFLSFTPLVEPL
                     SLDEAFLDVSGSVKLFGDPVTIAGTVKERIAELGLTCTVGVAPNKFLAKLASTRGKPD
                     GLLVIPPDRIEAFLHPLPVRALWGVGEATGETLRRLGIKTVGELAGIPRRTLERAVGD
                     SLGAHLAHLAAGLDDRPVTPHEPAKSVGSEETFAADLDAHDDILRELLRLSDRTAGRL
                     RSKGLCGRTVTIKVRWSSFQTITRSKTLPAEVDTAAEIYAVARGLYEKLAPATPRIRL
                     LGVSVTSLAAGPPRRQMDLLGGRPGRPGWHAASAAIDSIRERFGSSSVGPATLVDRG"
     gene            6118..6492
                     /locus_tag="VEV43_07225"
     CDS             6118..6492
                     /locus_tag="VEV43_07225"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HYP23346.1"
                     /translation="MPLSEREQRILDEIEKSLYQEDTGFARERSEKPRPAGKAAATVR
                     KGVAMFLAGLLVLFAFFMTKQVWVGALAFGSMVWGIVVVAGALRGEASSSPGRTVSDV
                     GDRIGRAAAEFERKLKERYKRQ"
BASE COUNT         1003 a         2178 c         2322 g         1151 t
ORIGIN      
        1 ggctgcaccg agttgaacgg cctggtgcga aaaatcaccg gaggggaagc tgcaccccca
       61 gtcgggagcg ccgttgttga cgaacccaac cctgattgct tcgctggcct ttatgaacat
      121 caacttgttc cgccccgcgg tgccccggcc aggaacgagt tcggcgggtt cgggagctgt
      181 gcgtgagcaa tacgtccccc aggcgccgag ttgagcgtcg tttccgagca atgctgggat
      241 cgcgtcgcgg atgccgaggg cgctcgccaa ctgagacgcg aactcgtcgt gcggcgcgat
      301 cggcggccac cccctgaggg cgtcgcgcga gaggaccgtc cccgtcgtgt ggtccacggg
      361 ggcggggatt gcaagtccga ttccgcggat ttcggtcccc cctatgcccg cgtcgcgtag
      421 agcatccgcc gccaggtcca ctgcctcgcc gagggtcttg gagaaagggt tctccacgat
      481 acggtggtcg tacgggactc ggatttcgag cattggcccg tcaggtggct caacggcgat
      541 ggcgatcgcg tagaaaccga gttccacgcc cacgagaacg cccgcggccc tgcccaagga
      601 gatcacctgt ggtctgcgtc cgacgtggtc gccgggacca gccgacttgg tgaggacgtt
      661 caagttcacc aactgatcga cgaggttcgt cacggtccct ctcgagagac cggtggcctc
      721 ggtcatctcc ttgacggtca actgctttcg gcgctggact acctcgagca gccgcgaggc
      781 gcttctcatc gagccgttcc gttgccacga ggtggtcact accacgcccc ctactgcgct
      841 catcgggcag ctttgccgcg tctttcgggt gttcacggta aggcgctgcg gttcgatccg
      901 taaaggtctt cgacgaaatc tgtcgaaatg ttgtacctac cgggcgaagc gttcggcgga
      961 agtcccgcaa aaagaataca tagggttggg gaaaatcttg accatatgga gcaaaactcc
     1021 gcactcttac ctaatgagca cggacggcga cgtcctcgaa cgacccagcg cttctagcgg
     1081 gcccccgacg agtggggacg ggccgcccgg tccgactccc ggtgggacgg ggccggggcg
     1141 tccgagttca ggcaggcctg ggcctgatgg gccctgggtc gtcgtgcttg cagccctgtt
     1201 ctctgcggct gcttcggttg tcatgacgga gctagacgtt ccgatctggt cctacttctt
     1261 gattctcgtc ttgaccggag gcggactgtt cctcttcttc cggacgaagc cgctccgggt
     1321 gcgagggctc gtattagctg tcctggtaac ggtcgccgcg atcgtcgctg gtatcgccgg
     1381 aacgacgtgg cttcagaact ggcggtgctc gcgtggggtt ggacgagggt tggaaccggc
     1441 atccaaggaa atgttccaac taacttacgc acggggcggg cgagaccgct tcggttgtcc
     1501 ggatggcgca gcgttcgcgc gtgagggcgg gatcttccaa ctcttcagcg gaggggactc
     1561 ggggctggga gcgatagcgg gggaacatcg ggagccgccg gtccctgttt tcggtccgct
     1621 ctggaggatc tacgagtcca tcggaggagg tctcgcagac gctgcatctc tgggaggtat
     1681 tccggctgag cgagatcgcg agggaaagtc gactgtcgtg tatttgaccg atggtgactg
     1741 cggcgagggg gcgctctaca aaccgcgtcc cggaaggccc cattgggtta gggccgaggt
     1801 ttacgaaaaa tacgctcgcc tcgctgggca cctagggttt ctcggtttcc cagtttcggg
     1861 gcctcggata gagggtcaga atgtacgtca gaagtttgag ggaggcgtca ttcgcgtgcg
     1921 agcggaggac gtgacttgtt acttggtgga ctagttagat cgcctccgac gggtctaagc
     1981 ccgaaccccg cgcaacgacc gcttttagca ccgcaacgcg cctccttcgg tgcctcggaa
     2041 attgtcagac gtttgtctga caaatcacga gggtggtgcg gggagcttct gtcggagctt
     2101 cagcaccggc tcgaagaggt ccccgtgctc ctctatccgg gccaggacct gatccgactc
     2161 gaagacgagg gccgccgggt cgtccgaaga gagagcggcc tcgacctcgt cccaggtgac
     2221 cggggtcgag accgtcgggc gggttttcgc ccgcagcgag tacaccgcca ccgtcgtctt
     2281 gtggtcgtcg ttctggctcc agtcgatcag gaccttgccg cggcgggcct ccttcgactg
     2341 cttggtcacg atcgcgtccg ggtgctcgga ctcgagccgg agcgcgaccg agtgggccca
     2401 ggccctcgtg tcgtcgaacg tcgcagaaag cgagaccggg acgaacatct ggatcccctt
     2461 cgagcccgag gtcttggcga agctctccag gccgaggtcg gagaacagag cccggatcca
     2521 cagcgcgacc cgggcgcagt cgagcaggtc ggtgccttcg ccggggtcga ggtcgaacac
     2581 gagggcccgc gggcggtcga ggcggtccgc gcgggcaagg gacgtgtgga gctcgaggtt
     2641 ggcgaggttc gaggcccacg cgagggccgc gcggtcgttc agcaggcagt agttgatcgt
     2701 cttgccgtcc ttgcggacca tcggggcggt cccgatccag tccgggcggt gcttcgggca
     2761 ctgcttctcg tagaagaagt caccctcgac gccgttcggg tagcgcttca aggtgacgag
     2821 ccggttcttg acgtgcggga ggaggaccgg ggcgacgtcc tggtagtagc gcgcgacgtc
     2881 gagcttcgtg aagcccgtct cgggatagaa gaccttgtcg gggttgctga cggagacctt
     2941 ccggcgcccc gcgagtattt ccactatcga gaggcgccgc ccacccgagt gagcagctcc
     3001 ttcacgcggc ccctgcctcc ggcctttggg tcgagcggtg gcgtgtccat ccccgcacct
     3061 tcgcgcaggc cctccatcag gtcgaggatc gcgtccgccg cgtcgtggcg cggcttccac
     3121 ccgagctcgg tccgtgcccg ggtggtgtcc atgatcggaa cctggagagc catgtcgagc
     3181 catcccgagg gggtgggctg gagcctcagc ttccacgtca cgtcggcgcc gatcttcagc
     3241 acgctcgccg ggatcttgac cggccgggcg ccgaggagct cggcgagccg cttcatgtcc
     3301 atgaccggct gggtcgcgac gttgaaggcc ccgctgacgc ccttcacgat cgcgagccgg
     3361 taggcgtccc cgacgtcgta ggagtggacg acctggaacc gcagcgcgtc tatgtcgggg
     3421 aggaagggga tgaggctccg tcggatcaag gtcccgggca acagggggcc gaagaacagc
     3481 cggcggacct ccgccgcgga ctcgcgcttg aagatcagcc cgggccgcag ccgtacgacg
     3541 cgcatgcggg ggttcgcccg ctcgacctcg tcgaggatcg actcggtcgc ggccttgtgg
     3601 cgggagtaga acgacgactc gacgccctcg acgggccagc tctcgtcgac gccggagtcg
     3661 tccttcggcc cgggggagta cgccccgatc gaggacgcgt agacgagcga cgggaccccc
     3721 gcggccgcag ccgcctcgaa gacccgcctg ctcccgtcga cgttcgtcgc gtggaggatc
     3781 cgcaggtcgc gggacggctg gatcagccag gcgaggtgga cgacgacgtc gcagcccgag
     3841 aacagccgga cgaggtcgtc cgtgacgatg tcggcccgcc gccacgtgac cttgggcacg
     3901 cgcatggccg gctcgcgccg ggcgatcccg aggatcgacc cgaccgccgg ctcgttctgc
     3961 agcgaccgga tgaggctcgt cccgacgttg cccgtcgctc cggtgatcgc gaccttaagg
     4021 ccgtgcgtgc cgaccacccc ggctactcgg gcttcgcgat cacgttcgcc atcgcgggca
     4081 tgagcatcag gcccccgtcc acgacgtacg aggcgccggt cgtgtacgag gcctcgggcg
     4141 aggcgaggtg cgcgatcacg tgcgcgacct cccaggcgtc gccgggccgc cccatcggga
     4201 tgcccgggcg ctcgacctcg gccgggtcga cgtcttcgtt gccggtcatc gccgtggcga
     4261 tctcgccggg cgcgacggcg ttgaccgtga tgccgtgctc ggcgagctcg agcgccatga
     4321 ccttcgtcag caggccgagc ccgcccttcg cggtgcagta cgcggccgag ccctccttcg
     4381 gagtgtgctc gtggaccgag gtcacgttga cgatccggcc ctcggtcttg ttcgcgacca
     4441 tgatccgggc ggccctctgg gcgcagagga acgctccgtt gaggtcgacg tccaggacct
     4501 tgagccagtc cgcgtagtcc tgctcgagga acggggtgct gtcaccggtg ccggcgttgt
     4561 tcacgaacac gtcgagcccg cccagctccg cggccagctc gtcgacgacg tccgccgccc
     4621 cgggcaggtt cgtgaggtcc aggtgccgga cctcggcccg ccggccgagc gcgccgacct
     4681 cgttcgccgt ctccttggcc cccgcctcgt ccgagttcca cgtgatgccc acgtcgcacc
     4741 cggcgcgggc gagggcgacg gcggtcgcct tgccgatgcc ggagtccgag gccgtgacga
     4801 tcgcgatcct tctgtccatt ggaagcggct ccttggttgg tcggtgtccg tgacgactac
     4861 ccgctgaagc cgcggcgtac gctcgtcggg atgaaggtcg ccaccccgat cctgcacgtc
     4921 gacatggacg cgttctacgc gtcggtcgag gtgatcaagg acccgtcctt gaagggcaag
     4981 cccgtgatcg tgggggggca ggggggccgc ggcgtcgtca cgtcggcctc ctacgaggcg
     5041 cgcgagttcg gcgtccgctc cgcgatgccg acgatccagg cccggaggct ctgcccccgc
     5101 gggatcttcc tgccgaacga cttcgcggcg taccaggacc tgtcggcgaa gatccgcgag
     5161 gtcttcctgt cgttcacgcc gctggtcgag ccgctgtcgc tcgacgaggc cttcctcgac
     5221 gtctcgggct cggtgaagct cttcggcgac cccgtgacga tcgccggcac cgtcaaggag
     5281 cggatagcgg agctcggcct cacctgcacc gtcggcgtcg cgcccaacaa gttcctggcg
     5341 aagctcgcct cgacgagggg caagcccgac ggcctgctcg tgatcccgcc ggaccgcatc
     5401 gaagcgttcc tccacccgct ccccgtgcgc gccctgtggg gggtgggcga ggcgaccggc
     5461 gagacgctgc gccggctcgg catcaagacg gtcggcgagc tcgccgggat cccgcggcgg
     5521 acgctcgagc gcgcggtggg ggactcgctc ggggcccacc tcgcccacct ggcggccggg
     5581 ctcgacgacc gcccggtcac cccgcacgag ccggcgaaat cggtcggctc cgaggagacc
     5641 ttcgccgccg acctcgacgc gcacgacgac atcctgcgcg agctcctgag gctgtccgac
     5701 cggaccgcgg ggcgcctgcg gagcaagggg ctctgcgggc ggaccgtgac gatcaaggtc
     5761 cgctggtcga gcttccagac gatcactcgg tcgaagaccc ttccggccga ggtcgacacc
     5821 gcagccgaga tctacgcagt tgcgcgcggg ctgtacgaga agttggctcc ggctacgccc
     5881 cgcattcggc tcctcggcgt cagcgtgacc tctctggccg ccggcccccc tcgccgccag
     5941 atggacctgc tgggcgggcg tccggggcgc cccgggtggc acgcggcgtc ggccgcgatc
     6001 gactcgatcc gggaacgctt cggatcctcc tcggtcggtc cggcgacgct cgtcgatcgc
     6061 ggatgacgcg ccggaacctg gctccgtata cggagccgaa cctataatcg gcagacagtg
     6121 ccgctttcgg agcgcgagca gcgcatcctc gacgagatcg agaagagcct ctatcaggag
     6181 gacacgggct tcgcgcgcga gcgctcggag aagcctcggc ccgccggcaa ggcggccgcg
     6241 accgtacgca aaggcgtggc gatgttcctc gccgggttac tggtgttgtt tgcgttcttc
     6301 atgacgaagc aggtgtgggt gggggcgctc gcgttcggct cgatggtctg ggggatcgtc
     6361 gtcgtcgcag gcgcgctgcg cggcgaggcg tcgtcttcgc cggggcgcac ggtgagcgac
     6421 gtcggggacc gtatcgggcg tgccgccgcc gagttcgagc gcaagctcaa ggaacgctac
     6481 aagcgccaat agtcacagcg cgaaaacttt tcgaagttcc tcggccattg cggcgtgctg
     6541 ggggttgccg cgtcgcgaga gggcgcgcta gattccacaa gtcgttcaga aagtcacgaa
     6601 tcaaacgggc tcacttcgcg gtgcatcgtt gcccgtcgcg ggttgggatc tcct
//
LOCUS       DASQDO010000253         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_25475, whole genome shotgun sequence.
ACCESSION   DASQDO010000253 DASQDO010000000
VERSION     DASQDO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25475"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VEV43_07230"
     CDS             complement(<1..1305)
                     /locus_tag="VEV43_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYP23347.1"
                     /translation="MRVATAGGGPGGLYFSLLLAKARPGARIEVFEKNPRGATYGWGV
                     VFSDRTLASFREADAETYEQIVDAFALWDVIEVRYKDDTVRCGGQVFSGMARRRLLDI
                     LQARCEELGVTLRFETEVGPEDVGDYDLVVAADGIHSRFRTAHAETLGPRIVEGRARY
                     VWFGTTCPFDSFTFVFRPTEHGLFQAHVYPFDGTTSTFIVECDEETWTAAGLDRAGER
                     ESIELCERVFAADLRGHSLLSNQSRWLSFPTLRTRRWWHGRTAFLGDAVHTAHFSIGS
                     GTKLAMEDAIALAREVARKPSLPDAFGAYELERKGVVERFQQAARESQQYFESTSRYM
                     DLDTEQFVFHLLTRSGRVGYDDLRLKDAALIDRVDSWFARRAGREVARFAPPPAFAPL
                     RLGASELTNRVAAVASSTDSCEDGMPDELHGAALARAAGEGAS"
     gene            complement(1302..2120)
                     /locus_tag="VEV43_07235"
     CDS             complement(1302..2120)
                     /locus_tag="VEV43_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYP23348.1"
                     /translation="MRVGVGLPNAVPGTAAETLVEWAERADAGPFSSVGVVDRVRYEG
                     FDALSALATAAAVTTRVTLTTMILIAPLRKGAVLAQEAATLHAVSGGRLVLGLGLGAR
                     RDDYEQVGAPFRGRASRFGRQLETLRSWWDDDASPGLLVGGGSDAAFARVARFADGYV
                     HGGGPPRAFARAAEKARAAWFDSGRPGEPRLWGQGYFALGDAAERGRAYMRDYYAFTG
                     SFAERIAEGMLSTPQAVVSFVKGYADAGCDEVVLFPAVSEPRQLDLLADALAAL"
     gene            2179..2610
                     /locus_tag="VEV43_07240"
     CDS             2179..2610
                     /locus_tag="VEV43_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HYP23349.1"
                     /translation="MPESFASLTIQRRIEWSDTDASGRWHNTAAFRMVEWAETALLER
                     LGILDDVYAYLPRAHVEADFLCALDFRDLVDATITVTNVGTTSLAYDFTIDRRGERCV
                     TMKVVAVLIDRDRNARPWPDRHRALLTQSGPQPPELFVVES"
     gene            2624..>3010
                     /locus_tag="VEV43_07245"
     CDS             2624..>3010
                     /locus_tag="VEV43_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23350.1"
                     /translation="MNALDFLGLDALLSDEEKMVRDSVRRFVSDRVLPGIEKWFEEGE
                     FPHEVAPELGRLGLLGMHLDGYGCAGTNAVSYGLACLELEAGDSGFRSFVSVQGSLAM
                     FPIWRYGSEEQKQEWLPRMAAGEAIGC"
BASE COUNT          466 a         1127 c         1003 g          414 t
ORIGIN      
        1 cgaggccccc tcccccgccg ccctcgcgag cgcggcgccg tgcagctcgt ccggcatccc
       61 gtcctcgcag gaatccgtcg acgaagcgac tgccgcgacc cggttcgtta gctccgaggc
      121 ccccagccgg agcggagcga aggccggggg cggtgcgaac cgagccacct cccgtccggc
      181 ccggcgggcg aaccacgagt cgacccggtc gatcagcgcc gcgtccttca gacgcaggtc
      241 gtcgtacccg acgcgcccgc tgcgcgtgag caggtggaag acgaactgct cggtgtcgag
      301 gtccatgtac ctcgacgtgg actcgaagta ctgctggctc tcgcgcgctg cttgctggaa
      361 ccgctcgacc acgcccttcc gttcgagctc gtacgcgccg aaggcatccg gcaggctcgg
      421 cttgcgggcg acctcccgcg cgagcgcgat cgcgtcctcc atcgcgagct tcgtcccgga
      481 cccgatcgag aagtgcgccg tgtgcacggc gtcgccgagg aacgcagtcc gcccgtgcca
      541 ccaccggcgc gtccgcagcg tcgggaacga gagccagcgc gactggttcg acagcaacga
      601 gtggccccgc aagtcggccg cgaacacgcg ctcgcagagc tcgatcgact cacgctctcc
      661 ggcccggtcg aggcctgcgg ccgtccacgt ctcctcgtcg cactcgacga tgaacgtgct
      721 cgtcgtcccg tcgaacggat agacgtgggc ctggaacagc ccgtgctcgg tcggtcggaa
      781 cacgaacgtg aacgagtcga acgggcacgt cgtgccgaac cacacgtaac gggcccggcc
      841 ctcgacgatg cgcggcccca gcgtttccgc gtgcgcggtc cggaaccggc tgtggatccc
      901 gtcggccgcg acgacgaggt cgtagtcacc gacgtcttcc ggcccgacct cggtctcgaa
      961 ccgcagcgtg acgccgagct cctcgcaccg tgcctggagg atgtcgagca gccgccggcg
     1021 cgccatcccc gagaacacct ggcccccgca cctgaccgtg tcgtccttgt agcggacctc
     1081 gatcacgtcc cacagcgcga acgcgtcgac gatctgctcg taggtctccg cgtcggcctc
     1141 gcggaacgaa gcgagagttc ggtcggagaa cacgacgccc cacccatacg tcgcgccgcg
     1201 cgggttcttc tcgaacacct cgatgcgtgc gccgggccgc gcctttgcca gcaggaggct
     1261 gaagtacagg cctccggggc ctcccccggc tgtcgcgacc ctcacagcgc ggccagcgcg
     1321 tccgcgagga ggtcgagctg ccgcggctcg gacaccgccg ggaacagcac gacctcgtcg
     1381 cacccggcgt cggcgtatcc cttgacgaac gacacgacgg cctgcggggt cgacagcatc
     1441 ccctcggcga tccgctcggc gaacgagccc gtgaacgcgt agtagtcgcg catgtaggcg
     1501 cgtcccctct cggcggcgtc gccgagcgcg aagtacccct gcccccacaa acgcggctcg
     1561 ccgggtcgtc cggagtcgaa ccacgcggcg cgcgccttct cggccgcgcg tgcgaacgcc
     1621 cgcggcggcc cgccgccgtg cacgtagccg tcggcgaacc gggcgacccg agcgaacgcc
     1681 gcatcgctcc ctcctccgac gagcagcccc ggcgaggcgt cgtcgtccca ccacgaccgc
     1741 agcgtctcga gctggcggcc gaacctcgac gcgcgccccc ggaacggtgc gcctacctgc
     1801 tcgtagtcgt cgcggcgagc gccgaggccg agcccgagga cgaggcgtcc gcccgatacg
     1861 gcgtgcaagg tcgcggcctc ctgggcgagg acggcacctt tgcgcagcgg agcgatcagg
     1921 atcatcgtcg tcagcgtcac ccgcgtcgtc accgcggccg cggtcgcgag cgcggacaac
     1981 gcgtcgaacc cttcgtagcg gacgcggtcg acgactccga cgctcgagaa agggcctgcg
     2041 tcggcgcgct cggcccactc gacgagcgtc tccgccgcgg tcccggggac cgcgttcgga
     2101 agaccgacgc cgacgcgcaa gtagagtcac ctcctagaag acgagcgacc aacctaaacc
     2161 cacgaaagcg gccgccccat gcccgagagc ttcgcgtcgc tgaccatcca gcgccggatc
     2221 gaatggtccg acaccgacgc gtcggggcgc tggcacaaca ccgcggcgtt ccgcatggtc
     2281 gagtgggccg agaccgcact gctcgaacgc ctcggcatcc tcgacgacgt ctacgcgtac
     2341 ctgccgaggg cccacgtcga ggccgacttc ctgtgcgcgc tcgacttccg cgacctcgtc
     2401 gacgcgacga tcacggtcac gaacgtcggg acgacgtcgc tcgcctacga cttcacgatc
     2461 gaccgtaggg gcgagcgctg cgtgacgatg aaggtcgtcg cggtcctgat cgaccgcgac
     2521 cgaaatgctc gcccgtggcc cgaccggcac cgggcgctgc tcacgcagtc cggcccccag
     2581 cctcccgagc tgttcgtagt cgaaagctag tagcgtcgcg gcgatgaacg cactcgactt
     2641 cctcggcctc gacgctctct tgagcgacga ggagaagatg gtgcgcgaca gcgtgcgccg
     2701 cttcgtgtcc gaccgggtgc tgcccggcat cgagaagtgg ttcgaggagg gcgagttccc
     2761 gcacgaggtc gcccccgagc tcgggcgtct cggcctgctc gggatgcacc tcgacggtta
     2821 cggctgcgcc gggacgaacg cggtcagcta cggcctcgcg tgcctcgagc tcgaagccgg
     2881 cgacagcggc ttccgcagct tcgtctccgt ccagggctcg ctcgcgatgt tcccaatctg
     2941 gaggtacggg tcggaggagc agaagcagga gtggctcccg cgcatggccg ccggcgaggc
     3001 catcggctgc
//
LOCUS       DASQDO010000254         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_698610, whole genome shotgun sequence.
ACCESSION   DASQDO010000254 DASQDO010000000
VERSION     DASQDO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698610"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VEV43_07250"
     CDS             complement(<1..989)
                     /locus_tag="VEV43_07250"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYP23351.1"
                     /translation="MLIGGETVEGAGAPVAVVNPATEEAFDEVRSATPGQVMDATKAA
                     RAAFRGWKRTPAPERGEMLHGLASWINEHSDDLATTLTREGGKPRVENRDEMGWSAAC
                     LDFYAEIGRLERGRVVPSGEADQLSLVIKEPFGVVAAIVPWNYPILLLMWKLAPALAA
                     GNTVVLKPSPYTPLSTLQMSEAFASLFPPGVVNVVTGEAEAGAALVDSPDVALVAFTG
                     SVETGKNVMRSAAESLKKVHLELGGKDAFIVCDDVDLEVAARGAVWAAYLNSGQVCTS
                     AERFYVLPQVYDDFVDAFVAEAQKVVVGDPMEDATDMGPLVRGIQRDRVEAQLA"
     gene            complement(990..2318)
                     /gene="gabT"
                     /locus_tag="VEV43_07255"
     CDS             complement(990..2318)
                     /gene="gabT"
                     /locus_tag="VEV43_07255"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HYP23352.1"
                     /translation="MDEPIPGPSSRRLIERREAAVPRAVFNTLPVFVASGDGARLVDV
                     DGNEFVDFAGGLGVLNVGRANPRVLEAVHKQADAFLHECFHVAMYEGYVELAEALNRI
                     TPGDHPKKTMLANSGAEAVENAVKIARLSTGRDSVLGFSNAFHGRTLMGMTLTAKEMP
                     YKRGFGPFAPEVHRVPFAYCYRCPFGLEHPSCGMACAEHTADVIDSELGRENVACIVV
                     EPVQGEGGFVVAPDDFLPALRAICTERDILLVDDEIQSGMGRTGKMFAADHYGVVPDI
                     VTTAKSLGAGLPISGVTGNADVMDAPHVGGLGGTYGGNPVACAAALAVIEELTTTDLL
                     ERAERQTQIVRSRLDPLEDALGLVGEVRGLGPMIGIELVTDEITKEPAKVATAAVVKR
                     CHDNGVLVLKAGTYDNVVRLLAPLVISDDDLHKGLDVLVEALEWANRGVG"
     gene            2408..3955
                     /locus_tag="VEV43_07260"
     CDS             2408..3955
                     /locus_tag="VEV43_07260"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HYP23353.1"
                     /translation="MTVRDLLALPGLGLTVVAGEAGLDRPIRWVHTSELQDPTPWLSG
                     GELLLTTGMGLTGSAALQRSYIRRLISADLAGLGFGVGFGADEVPAAIKRAADSGGFP
                     VIEVPYPVPFIAIAEAVSSALAEDRLREARMSVEVHERLAALVSRGGGPADVLEEAVA
                     IAGGWALLFDVRGGVLARAAARAVAVPDEAKLWKTLPPPGDSTSTAADQGPGSSFVAL
                     AVDAGGRREGWAVFGKDRRIEPRDRIVARHAVTVLGLLLTSRRAVVDAERRIAGDVLV
                     EAIAGRVDGSELTRRLELVGFPRGSAVVALVVDVGSVPEPALDEAAWLLDGALGVRQV
                     PGRTALVEGRVIALVASQEPERLAGLLLEEARSWPVVAGDVRIGVGGACPAADLRRSY
                     LEAVFALRAAGPDVPIASPRDLGSYAVLLGGQPREALEGFVRSVLGPLIDRDRARSSD
                     LVASVRAFIESGGRWEPGAESLAIHRHTLRYRVRQAEELLGRDLASAEDRMEIWLALK
                     AMEVLER"
     gene            complement(3976..>4359)
                     /locus_tag="VEV43_07265"
     CDS             complement(3976..>4359)
                     /locus_tag="VEV43_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23354.1"
                     /translation="PVAPALVLARSICEAHGLEFHLASERSISDNLWENVAGHMWGSR
                     YGIGFFEDRKNEGLNPNLAIEIGSMLMTGRRCALLKDATVTRMPTDLVGKIYKDIDLD
                     APATVESALHVWIREDLALGPCSDC"
BASE COUNT          626 a         1458 c         1571 g          704 t
ORIGIN      
        1 gccagctgag cttcgacgcg gtcgcgctgg atccctcgaa cgagcgggcc catgtccgtc
       61 gcgtcctcca tggggtcgcc gacgacgacc ttctgcgcct ccgcgacgaa tgcgtccacg
      121 aagtcgtcgt acacctgcgg caggacgtag aagcgctcgg ccgacgtgca gacctgcccg
      181 ctgttgaggt aggcggccca gaccgcgccg cgcgcggcga cctcgaggtc gacgtcgtcg
      241 cacacgatga acgcgtcctt gcctccgagc tcgaggtgga ccttcttgag gctctcggcc
      301 gcggaccgca tgacgttctt gcccgtctcg accgagccgg tgaacgccac gagcgcgacg
      361 tcgggcgagt cgacgagtgc cgctccggcc tcggcctcgc ccgtgacgac gttgaccact
      421 ccgggaggga acagcgacgc gaacgcctcc gacatctgga gcgtcgagag cggcgtgtac
      481 ggcgacggct tgagcacgac ggtgttgccg gcggcgagcg ccggggcgag cttccacatc
      541 agcagcagga tcgggtagtt ccacggcacg atcgccgcga cgacgccgaa cggctccttg
      601 atcacgagcg agagctggtc ggcctctccc gaggggacta cgcgtccgcg ctcgagcctc
      661 ccgatctcgg cgtagaagtc gaggcacgcg gcgctccagc ccatctcgtc acggttctcg
      721 acccggggct tgccgccctc gcgggtgagc gtcgtcgcga ggtcgtcgga gtgctcgttg
      781 atccacgacg cgaggccgtg gagcatctcg ccccgctcgg gggccggggt gcgtttccag
      841 cctctgaagg cggcgcgtgc ggctttggtc gcgtccatga cctgcccggg agttgccgag
      901 cggacctcgt cgaacgcctc ttcggttgcc gggttcacga ccgcgacggg tgcgcccgcg
      961 ccctcgaccg tctctccgcc tatcagcatc tagcccacgc cccggttggc ccattcgaga
     1021 gcctcgacga gcacgtcgag gcccttgtgg aggtcgtcgt cggagatcac cagcggcgcg
     1081 agcagccgca cgacgttgtc gtaggtcccg gccttcagca cgagcacacc gttgtcgtgg
     1141 cagcgcttga cgacggcggc cgtcgcgacc ttggccggct ccttcgtgat ctcgtcggtg
     1201 acgagctcga tcccgatcat cgggccgagc ccccggacct cgccgacgag gccgagagcg
     1261 tcctcgaggg gatcgagccg cgacctgacg atctgcgtct gacgctcggc tcgttcgagg
     1321 aggtcggtcg tcgtgagctc ctcgatcacc gcgagcgcgg ccgcgcacgc gaccgggttg
     1381 ccgccgtagg tcccgccgag cccgccgacg tgcggcgcat ccatcacgtc ggcgttcccg
     1441 gtgacgcccg agatcggaag tccggccccc agtgacttcg ccgtcgtcac gatgtcgggg
     1501 accactccgt agtggtcggc ggcgaacatc tttcccgtgc ggcccatgcc gctctggatc
     1561 tcgtcgtcga ccagcaggat gtcgcgctcg gtgcagatcg cccgcagggc cgggaggaag
     1621 tcgtccggcg cgacgacgaa ccctccttcc ccctgcacgg gctcgacgac gatgcacgcg
     1681 acgttctcgc ggccaagctc cgagtcgatg acgtcggccg tgtgctccgc gcacgccatc
     1741 ccgcacgacg ggtgctcgag cccgaagggg caccggtagc agtacgcgaa cgggacgcgg
     1801 tgaacctccg gagcaaaggg gccgaagccg cgcttgtagg gcatctcctt cgcggtgagc
     1861 gtcatgccca tcagcgtccg tccgtggaac gcgttcgaga acccgagcac actgtctctg
     1921 ccggtggaga gccgcgcgat cttcaccgcg ttctcgacgg cctcggcccc cgagttcgcg
     1981 agcatggtct tcttcgggtg gtcgcccggc gtgatgcggt tgagggcctc ggcgagctcg
     2041 acgtagccct cgtacatcgc gacgtggaag cactcgtgca ggaacgcgtc cgcttgcttg
     2101 tgcacggctt cgagaacgcg cgggttcgcg cggccgacgt tgaggacgcc gagcccaccc
     2161 gcgaagtcga cgaactcgtt gccgtcgacg tccacgagcc gcgcgccgtc gcccgacgcg
     2221 acgaagaccg ggagcgtgtt gaagaccgcc cgcgggaccg cggcctcccg gcgctcgatc
     2281 aaccgccgcg agctcgggcc cgggatcggc tcgtccatcc accgacggta tcgccgcctg
     2341 cgccgtgccg agtcgctgcg ccgaggcgag ttcgggacgc ccggtttgta cactctggaa
     2401 ggtgccgatg acagtgcggg atctcctcgc cctccccggc ctcggcttga cggtcgtagc
     2461 gggcgaggcg gggctcgatc gccccatccg ctgggtccac acgtccgagc tgcaggaccc
     2521 gacgccgtgg ctgtcgggcg gcgagctgct gctgacgacc gggatgggcc tgaccggctc
     2581 cgccgcgctc caacgctcct acatccggcg gctgatctcg gccgacctcg ccgggctcgg
     2641 cttcggtgtc ggcttcggtg ccgacgaggt ccccgcggcg atcaagaggg ccgcggactc
     2701 aggcgggttc ccggtgatcg aggtgccgta cccggtcccg ttcatagcga tcgcggaagc
     2761 cgtgtcgagc gcgctcgcgg aggaccggct gcgcgaggcg cggatgtcgg tggaggtgca
     2821 tgaacgcctt gcggctctgg tgtcgcgcgg cggcggtccc gcggacgtgc tcgaggaagc
     2881 ggttgcgatc gccggcgggt gggccttgct gttcgacgtg cgcgggggag tgctggcgcg
     2941 cgccgcagca cgggccgtcg ccgttcccga cgaggcgaag ttgtggaaga cgctgccgcc
     3001 gccgggtgac tcgacgtcga cggcggcaga ccagggaccc gggtcgtcgt tcgtcgcgct
     3061 ggcggtcgac gccggaggcc ggcgcgaggg ctgggcggtc ttcgggaagg accgccgtat
     3121 cgagccgcgc gaccgcatcg tggcgcgcca cgcggtgacg gtgctgggct tgttgctgac
     3181 gtcgcggcgc gcggtcgtcg acgcggagag acggatcgcc ggagacgtgc tggtcgaagc
     3241 catagccggg cgggtcgacg ggtcggagct gacgaggcga ctggagctcg tcgggttccc
     3301 gcgcggctcg gccgtcgtgg cgttggtcgt ggacgtggga tcggtgccgg agcccgcgct
     3361 cgacgaagcg gcgtggttgc tcgacggtgc gctgggagtg cgccaggtcc cgggccggac
     3421 tgcgctggtc gagggcaggg tgattgcgct cgtcgcgtcg caggagcccg agcgcctggc
     3481 gggtctccta ttggaggagg cgcgctcgtg gcccgtcgtg gccggggacg tccggatagg
     3541 agtaggaggc gcgtgcccgg cagcagacct gcgccgttcc tatctggagg cggtcttcgc
     3601 gctacgcgcc gcggggccgg acgtgccgat cgcgtcgccg cgcgacctgg gctcgtacgc
     3661 ggtcttgttg ggcgggcagc cgcgtgaagc gctggaaggc ttcgtgcgct cggtgctggg
     3721 cccgttgata gaccgggacc gggcccgttc gtcggacctg gtggcgtcgg tgcgagcatt
     3781 catagaatcg ggcggacgct gggagccggg ggccgagagc ctggcgatcc accgccacac
     3841 gctgcgctac cgggtccgcc aggcggagga actactgggc cgggacctag cgagcgccga
     3901 agaccggatg gagatctggc tagccctgaa agcaatggaa gtcctggaga ggtaagaggg
     3961 cagagagaag ggaactcagc agtccgagca cgggccgagt gcgagatcct ctcgtatcca
     4021 cacgtgaaga gccgactcca ccgtggcggg tgcatcgaga tcgatgtcct tatagatttt
     4081 gccgacgagg tctgtgggca tcctggtgac ggtcgcgtcc ttcaacagcg cacatctccg
     4141 accggtcatg agcatgctcc ctatctctat cgcgaggttg gggtttaaac cttcgttctt
     4201 cctgtcctcg aaaaagccga tgccgtaacg actaccccac atatggccgg ccacgttctc
     4261 ccagaggttg tcggatattg acctctccga cgcaagatga aactcaagac catgagcttc
     4321 acaaatcgag cgcgctagaa caagagccgg cgccactgg
//
LOCUS       DASQDO010000255         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1188253, whole genome shotgun sequence.
ACCESSION   DASQDO010000255 DASQDO010000000
VERSION     DASQDO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188253"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1134)
                     /locus_tag="VEV43_07270"
     CDS             complement(31..1134)
                     /locus_tag="VEV43_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23355.1"
                     /translation="MLTSAETARRDEFRSFARAEIAPRAAAFDRAGEVDEDLIVKLAG
                     AGYLGAADMDEVTFGLLCEEIGAACSSVRSLLTVQDMVARTIARWGSAEQKDQWLPRL
                     ASGDAIAGFCLTEPGTGSDAAAVTTSFEDAGDDWVVTGHKLWVTFGRRADVFLVVGRA
                     AAGPVAALVPRGVARLDLRPVDGQLGLRAAMLAEVVLDGCRVPKANTVAKPGFGFSHV
                     AGTTLDHGRYSVAWGCVGIARGCVEASAAYATERVQFGSPLRDHQLVRRMLADMQVST
                     DAARLMCIEAGRLRAVSDPRSLEATMAAKYFASTILPRLTNDAVQIHGANGVDPRFAV
                     ERHFRDAKIMQIIEGTDQMHQLNLAGVALRGSS"
     gene            complement(1154..1420)
                     /locus_tag="VEV43_07275"
     CDS             complement(1154..1420)
                     /locus_tag="VEV43_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYP23356.1"
                     /translation="MKDTVSSVKSAIHTFVDRFFGDTTLEDGQDMFETGYVNSMFALQ
                     LVQFVEGEFGITVDSDDLELDNFRTVSAIATFVERKKASSAVAG"
     gene            1555..>2536
                     /locus_tag="VEV43_07280"
     CDS             1555..>2536
                     /locus_tag="VEV43_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYP23357.1"
                     /translation="MVNEISSTESSSEDAGAVAQELAFPASFGQERLWVLEHLRADVP
                     LYMHSFAVRCRGPLDVDALRAAVATVVARHETLRTRFEIRAGSLVQVVVPHLDVDVEV
                     VDLTHLGAGAEDEARRLAESLDDEPVDLARPPLVRFRLLRVAADDHVLVVAIHHVVSD
                     GWSIDVLLRDLGTCYDARLAGDQPSLPDLPIQYADYAVWQREWLSGAELDKQVAYWSD
                     ALAGAPALLELPTDRPRPPVQSFDGATAPVSIPPDVVAALNEVAATAGATPFMAVTAA
                     FAAVLHRYASQTDVLLATPVAGRRRSEVEALIGFFVNTLVLRTDCSGNPSF"
BASE COUNT          369 a          916 c          876 g          375 t
ORIGIN      
        1 ccagggagag gcccaccgcc gcgatgccgc tcacgacgac ccccgcagcg cgacgccggc
       61 caggttgagc tgatgcatct ggtccgtgcc ctcgatgatc tgcatgatct tcgcgtcgcg
      121 gaaatggcgc tcgacggcga aacgcgggtc gaccccgttg gcgccgtgga tctgcaccgc
      181 gtcgttcgtc aggcgcggca ggatcgtcga cgcgaagtac ttcgccgcca tcgtcgcctc
      241 gagcgaacgg gggtcggaga ccgcgcgcaa ccggcctgcc tcgatgcaca tcagccgggc
      301 cgcgtcggtc gacacctgca tgtcggcgag catccttcgc acgagctggt gatcgcgcag
      361 cggcgagccg aactggaccc gctccgtggc atacgccgcg ctcgcctcga cgcagccgcg
      421 cgctatcccg acgcagcccc acgcgacgct gtagcgacca tggtcgagcg tcgtcccggc
      481 cacgtgggag aagccgaagc caggcttggc gaccgtgttc gccttcggca cgcggcatcc
      541 gtcgaggacg acctcggcca gcatcgccgc cctgagaccc agctggccgt cgaccggccg
      601 gaggtccaag cgggcgacgc cgcgggggac gagggccgcg accgggccgg ccgccgcgcg
      661 gccgacgacg aggaacacgt ccgcgcgccg gccgaacgtc acccacagct tgtggccggt
      721 gaccacccag tcgtcgccgg cgtcctcgaa cgacgtggtc acggccgccg cgtcgctccc
      781 ggtgcccggc tcggtgaggc agaacccggc gatcgcgtcg cccgacgcga gccgagggag
      841 ccactggtcc ttctgctcgg cgctgcccca gcgcgcaatc gtgcgcgcga ccatgtcctg
      901 gacggtcaga aggctccgca ccgacgagca ggccgcgccg atctcctcgc acagcaggcc
      961 gaacgttacc tcgtccatgt ccgcggcgcc gaggtatccc gcgccggcga gcttcacgat
     1021 caggtcctcg tccacctcac cggcgcggtc gaaggccgcg gcgcgcggag cgatctcggc
     1081 gcgcgcgaac gaacggaact cgtctcgccg cgcggtctcg gcggaggtca gcactgggat
     1141 tgaagcggcg cggctagccc gccaccgcgg acgaagcctt cttgcgctcg acgaaggtgg
     1201 cgatcgcgga gaccgtccgg aagttgtcga gctcgaggtc gtcgctgtcg accgtgatgc
     1261 cgaactcgcc ctcgacgaac tgcacgagct gcagcgcgaa catcgagttg acgtagccgg
     1321 tctcgaacat gtcctggccg tcttcgagcg tcgtgtcgcc gaagaaccgg tctacgaacg
     1381 tgtgtatagc ggacttaacg gatgaaaccg tgtccttcac tagcgtccgc cccttccggt
     1441 cacggcggga ttatagactc ctccctctgc tgggcctccg gaggaaacac cgccggtcgc
     1501 cggtgtggga tgcgggcggc ggggcccgca ttggggggaa ctattcgaga cccggtggtg
     1561 aacgagatca gctcaacaga gagctcctcc gaagacgcgg gcgcggtcgc gcaggagctc
     1621 gcgttccccg cgtcgttcgg acaagagcgc ctgtgggtgc tcgagcatct gcgtgccgac
     1681 gtcccgctct acatgcactc gttcgcggtg cgctgccgcg gccccctcga cgtcgacgcg
     1741 ttgcgtgccg cggtggcgac cgtcgtagcg cgacacgaga ccctgcgcac gcgcttcgag
     1801 atccgcgccg gctccctcgt gcaggtcgtc gtaccgcacc tagacgtcga cgtcgaagtg
     1861 gtcgacctca cgcatctcgg ggcgggcgcg gaggacgagg cgcggcggct cgcggagtcg
     1921 ctcgacgacg agccggtcga cctcgcgcgg cccccgctgg tgcgcttccg cctgctgcgc
     1981 gtcgccgcgg acgaccacgt gctcgtcgtg gcgatccacc acgtcgtctc ggacgggtgg
     2041 tcgatcgacg tcctcttgcg cgacctgggc acctgctacg acgcccggct ggcgggcgat
     2101 cagccgtcgc tgccggacct tcccatccag tacgccgact acgccgtctg gcagagggag
     2161 tggctgtcgg gggccgagct cgacaagcag gtcgcgtact ggagcgacgc gctcgccggt
     2221 gcaccggccc tgctggagct cccgacggac cggccgcggc cccccgtgca gagcttcgac
     2281 ggcgcaaccg caccggtgtc gatcccgccc gacgtcgtag ccgcgttgaa cgaggtcgcc
     2341 gcgacggccg gcgcgacgcc gttcatggca gtgaccgccg cgttcgccgc ggtcctgcac
     2401 cgctacgcgt cgcagaccga cgtgctgctt gcgacgcccg tcgcgggacg ccggcgttcg
     2461 gaggtcgaag ccctgatcgg cttcttcgtc aacacgctgg tgctacggac ggattgctcg
     2521 ggcaacccgt cgttcg
//
LOCUS       DASQDO010000256         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_576720, whole genome shotgun sequence.
ACCESSION   DASQDO010000256 DASQDO010000000
VERSION     DASQDO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576720"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VEV43_07285"
     CDS             complement(<1..1087)
                     /locus_tag="VEV43_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYP23358.1"
                     /translation="MGSSEGRVRSRLVGVAAGGVAVVFAAPFLYLLVRNVRDPGTAWS
                     ALSSGDAWGPLGRSVLLAVLVSASATVVGAGAAWLVARTDLPGARVWRVLLPLPLVMP
                     SFIGAFVLVAALAPGGLVEAALRPFGVDRLPGIGGLAGSFLVLTLFTYPYVYLPARAR
                     FRRLAPSLEESARLLGRGSWSTFWRVVLPQSRGAILAGTLLVFLYSVSEFGVVQLMRY
                     DTLTRSIYATRFLDPSRSFALSLQLGLLAVAVVVAERVVARGGGLERTESQRDHRVMV
                     LSLGRWRAAAVASLTALVGMALALPVGVLTWWAVRGAARGATSATGGFGDVAGLGRSI
                     LNTSFVSLAAAVVAVAAVLPVAYLIVRR"
     gene            complement(1074..2075)
                     /locus_tag="VEV43_07290"
     CDS             complement(1074..2075)
                     /locus_tag="VEV43_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYP23359.1"
                     /translation="MGTSRRILTALALLSAVAASACSGGRDALTIYSGRDEALVGPVL
                     EDFVDETGIDVDIRYGDSGELALLLNQEGDKTRADVFLSQSPGPTAAVAAEGGLAELP
                     SGTLELVDERWRGAGGRWVGVTARQRVLVYNSEMVAEDELPESVFDLVEPEYEGRVAV
                     APTNASFQDFVTAMRETEGDGATQEWLEGMAANGAQTYADNSSIVAAVGRGEIPMGLV
                     NHYYNYRALEEDPSLPTRNYVFPPDDLGSLLIDSTVGILDTTDHRDDAELFVEYLLDR
                     EAQEFFSRETFEYPLADGAAASEELPPLEELEGPDVDLTALDDLEATARLIEDSGLL"
     gene            2117..>3679
                     /locus_tag="VEV43_07295"
     CDS             2117..>3679
                     /locus_tag="VEV43_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HYP23360.1"
                     /translation="MAEQALDLRIRGLKWWWGGTLLALVVALLALRSVDASVFGETWR
                     AAVDTPLAVLAVTSVYGTAFLVRAWVWTRLAPGLRFGTAWAAIHVSLGANHVLPLRLG
                     EVFRVTTAVKRGGIPARVATSSTLFLRAADVVAVAGLAALLGPRLVDDLLGGLAWLPL
                     AGAAAVGVAGFVWLRRTASRTVAGTTGAVLGGGAAVAGWVLESAVIWQAARWAGIDLS
                     VADAVLVTAVTIAAQVFAVAPAGIGTYEAAATAAFVALGAGADAGLAAAIAAHALKTA
                     YSLVTGAVALFVPAPGAFGRLRLPAVAERPPVAPGEGPVVLFLPAHDEVESVADVVRR
                     IPPRVCGRDVIALVVDDGSADGTGEAALAAGAEVLTAPRNEGLGAAVRRGLAEGVARG
                     AAAVAFCDADGEYAPEELGALVEPILTGEADYVVGSRFAGRIDRMLPHRRFGNVVLTK
                     LLSFVARRRISDGQSGYRTLSARAAAEAELVHDYNYAQVLTLDLLGKGFRYAEVPISY
                     GFRTTGKTFVKLG"
BASE COUNT          496 a         1359 c         1359 g          465 t
ORIGIN      
        1 ggcgccgcac gatcaggtac gcgaccggca ggaccgccgc cactgcgacg accgcggcgg
       61 cgaggctcac gaacgacgtg ttcaggatgc tgcggccgag cccggcgacg tcgccgaagc
      121 cacccgtggc gctcgtcgcc ccgcgcgctg cgcctcgcac cgcccaccac gtgaggaccc
      181 cgaccggcaa cgccagcgcc atgcccacga gcgcggtcaa ggacgcgacc gccgccgccc
      241 gccaccgtcc gagcgacaac accatcacgc ggtggtcgcg ctgcgactcg gtccgctcga
      301 ggcccccgcc gcgcgcgacg acgcgctccg cgacgacgac cgctaccgcg agcaggccca
      361 gctgcaacga cagcgcgaac gaccgcgacg ggtcgaggaa cctcgtcgcg tagatcgacc
      421 gcgtcagcgt gtcgtagcgc atgagctgga cgacgccgaa ctccgacacg ctgtagagga
      481 agacgaggag cgtgccggcc aggatcgcgc cgcgggactg cggcaggacg acgcgccaga
      541 acgtgctcca cgagccgcgg ccgaggagcc gcgcggactc ctccagcgac ggcgcgaggc
      601 ggcggaagcg cgcccgggcc ggcaggtaca cgtacgggta ggtgaacagc gtgaggacga
      661 ggaacgaccc cgcgagcccg ccgatgccgg ggaggcggtc gacgccgaac gggcgcagcg
      721 ccgcctcgac gaggcccccg ggcgcgagcg cggccacgag cacgaacgcg ccgatgaacg
      781 acggcatcac gagcgggaga gggagcagca cgcgccagac gcgcgctccg ggcaagtccg
      841 tgcgcgccac cagccacgcg gcccccgcac ccacgacggt cgccgaggcc gacacgagca
      901 cggcgagcag cacgctccgt cccagaggcc cccacgcgtc acccgacgag agcgcggacc
      961 atgcggtccc ggggtcccgg acgttgcgaa ccagcaggta gaggaagggg gccgcgaata
     1021 cgacggcgac cccgcccgca gcgaccccta cgaggcggga acggacgcgg ccctcagagg
     1081 agcccactgt cttcgatcag ccgcgccgtc gcctccaggt cgtcgagcgc ggtcaggtcg
     1141 acgtccggcc cctcgagctc ttctagcggc gggagctctt ccgaagccgc ggcgccgtcg
     1201 gcgagcgggt actcgaaggt ctcgcggctg aagaactcct gggcctcgcg gtcgagcagg
     1261 tactcgacga agagctccgc gtcgtcgcgg tggtcggtcg tgtcgaggat ccctacggtc
     1321 gagtcgatca gcagcgatcc caggtcgtcg ggcgggaaga cgtagttgcg cgtcggcaga
     1381 gacgggtcct cctcgagcgc gcggtagttg tagtagtggt tcacgagccc catcgggatc
     1441 tcgccgcgcc ccacggccgc gacgatcgac gagttgtccg cgtacgtctg cgcgccgttg
     1501 gcggccatcc cctcgagcca ctcttgcgtc gcgccgtcgc cttcggtctc gcgcatcgcc
     1561 gtcacgaagt cctggaacga cgcgttcgtc ggcgcgaccg cgacccggcc ctcgtactcg
     1621 ggctcgacca ggtcgaacac cgactccggc agctcgtcct ccgcgaccat ctccgagttg
     1681 tagacgagga ccctctggcg cgccgtgacg ccgacccacc tcccgccggc gccgcgccac
     1741 cgttcgtcga cgagctcgag cgtgccggac gggagctcgg cgaggccccc ctccgcggcc
     1801 acggcggcgg tggggcccgg gctctgcgag aggaagacgt cggcgcgggt cttgtccccc
     1861 tcctggttga gcagcagcgc gagctcgccg gagtccccgt agcggatgtc gacgtcgatc
     1921 ccagtctcgt cgacgaagtc ctcgaggacg gggccgacga gcgcctcgtc gcgcccggag
     1981 tagatcgtga gggcgtcgcg gccccccgag caggcgctcg cggcgaccgc cgacaggagc
     2041 gcgagggcgg tgaggatgcg cctggaagtg cccatgtaag gtgaccctaa caagggaagg
     2101 acggtgaggc gaagaagtgg ccgagcaggc cctcgacctg cggatccgcg gtctcaagtg
     2161 gtggtggggc gggacgctac tcgccctcgt ggtcgccctg ctggcgctgc ggtcggtcga
     2221 cgcctcggtc ttcggggaga cctggcgggc ggcggtggac accccccttg ccgtgctcgc
     2281 cgtcacctcc gtgtacggga ccgccttcct ggtgagggcc tgggtatgga cccgcctcgc
     2341 ccccgggctg cgcttcggga ccgcctgggc ggcgatccac gtctcgctcg gcgcgaacca
     2401 cgtcctcccg ctccggctgg gtgaggtctt ccgcgtcacg acggccgtga agcgcggcgg
     2461 gatccccgcg cgggtcgcca cctcctcgac gctcttcctg cgggcggccg acgtcgtggc
     2521 ggtcgcgggg ctcgccgctc tgctcggccc ccgtctggtc gacgacctcc tcggcggcct
     2581 ggcctggctg ccgctcgccg gcgcggccgc cgtgggagtc gccgggttcg tctggctgcg
     2641 ccggaccgcg tcccggaccg tcgccggcac cacgggcgcg gtcctcggcg gcggggcggc
     2701 ggtcgcgggg tgggtgctcg agagcgccgt gatctggcag gccgcgcggt gggccgggat
     2761 cgacctctcc gtcgccgacg ccgtcctcgt cacagccgtg acgatcgccg cgcaggtctt
     2821 cgccgtcgcg cccgccggca tcggcacgta cgaggccgct gcgaccgcgg ccttcgtcgc
     2881 gctcggcgcc ggggcggacg ccgggctcgc cgccgcgatc gcggcgcacg cgctcaagac
     2941 cgcctactcg ctggtcaccg gggcggtcgc gctgttcgtc cccgcaccgg gcgcgttcgg
     3001 gcgcctgcgt cttccggccg tcgcggagcg cccgccggtc gctcccggcg aggggcccgt
     3061 ggtgctgttc ctccccgcgc acgacgaggt cgagtcggtc gcggacgtcg tgcggcgtat
     3121 cccgccgcgg gtctgcggcc gggacgtgat cgcgctcgtg gtggacgacg ggtcggccga
     3181 cgggaccggc gaggccgcac tcgcggcggg ggccgaggtc ctgaccgcac cccggaacga
     3241 gggcctgggg gccgcggtgc ggcgaggtct cgcggaaggc gtggcgcgcg gcgcggccgc
     3301 ggtcgccttc tgcgacgcgg acggcgagta cgcgccggag gagctggggg cgctcgtcga
     3361 gccgatcctc accggcgagg ccgactacgt cgtcggctcg cggttcgccg gacggatcga
     3421 ccgcatgctc ccccaccgcc gcttcggcaa cgtcgtcctg acgaagctgc tctcgttcgt
     3481 cgcgcgtcgc cggatctccg acggacagag cggataccgg acgctgtccg cacgtgccgc
     3541 agccgaggcc gagctcgtgc acgactacaa ctacgcgcag gtgctcacgc tggacctgct
     3601 gggcaagggc tttcgctacg ccgaggtccc gatcagctac ggcttccgga cgacgggcaa
     3661 gacgttcgtg aagctcggg
//
LOCUS       DASQDO010000257         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_454535, whole genome shotgun sequence.
ACCESSION   DASQDO010000257 DASQDO010000000
VERSION     DASQDO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454535"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VEV43_07300"
     CDS             complement(<1..752)
                     /locus_tag="VEV43_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23361.1"
                     /translation="MRTIVAGLRVLREAPLAMAPFAAEGLIAGVLVLTGAFPGDGAAA
                     ASAAVFPLDVFFDVKQALAHGRGWPWFAAAVALSIVVRSGALALTLWLKDARRAPLAV
                     AWVRCLRLAAGAAIALLPSAALFFAGVAIRYAPFVWVGAIAGFVPSLWFVRRAASLDA
                     GAGTPRGKGVPEIGTFLGYAFVVTMLGAAMSVLGGGMGRWAAAVLLACSAPLHALYLL
                     GWRTHLEEETYPAGGSIAVTVTVVGVVGLGAAT"
     gene            783..2372
                     /locus_tag="VEV43_07305"
     CDS             783..2372
                     /locus_tag="VEV43_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HYP23362.1"
                     /translation="MAAAEPELPVIELPARSRRAGWSGRDWLALSAVTIGSALLRLTR
                     LADPKHLIFDEVYYARDACWYVYAAPSVCGIDHESTPVHPPMSKWLIAVGIRLFGYDS
                     FGWRFTAAIAGVVTVALLYLLARKVLGSTLGATLAAGLLGVDFLHFVQSRTSMLDIFI
                     PMFGVAAMLFVAYDRDRMLALLDRPPDERPHGLLDRPWRLAAGAAGAGAVASKWSGGM
                     VLMIVILLTFAWEIGTRRRDGHPRPLRRFAREELPSIAVWLVILPVALYGLTYVGRIG
                     GSYTALPWTADAWVNNVWNQQFEMANFHDDLDATHSYQSPWWSWILLKRPVSYAYCGG
                     DSCDPPAPNGEVKEVLATGSPFVWGASILAVLFALWRWVRKWEWWKPKRDARTLVGGT
                     EGFVVAGFLLTYLPWIALTWNREAVFIFYILPTIPFMCLALAWVVTRIGWTWEAKAAV
                     ALFSLGAVGLFVFFYPILANVSIPEHHWRSRIQAFDDCEAPEPEESTSTVTSTVNGKK
                     VERIVTSTGDGGGPPAGWCWI"
     gene            complement(2369..2836)
                     /locus_tag="VEV43_07310"
     CDS             complement(2369..2836)
                     /locus_tag="VEV43_07310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYP23363.1"
                     /translation="MKTFRTLLIALLLLLGAACGDDTPSGSDAGGGGSDGCEEGAVVE
                     LDSGLEYEELECGDGTEVARGDVIEVFYTGTLDDGTEFDSNVGADPVKFALESGNLIE
                     GWVEGIPGMKEGGRRKLTIPSELGYGESGYPPDIPPNATLVFEVEIVSVEEAP"
     gene            complement(2846..3853)
                     /locus_tag="VEV43_07315"
     CDS             complement(2846..3853)
                     /locus_tag="VEV43_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23364.1"
                     /translation="MRRAVVAIAAVVVATGLTPPASATPLPRSIDEARALFEQPRDGG
                     SNQMFIQLVPPGYKGYRWTVLAYKKNPRAKTSLVLGFIRTGAQDTQSQRSEFRWELPR
                     GAMRMASDLRPVSLDTRRGMGNNGFVDMKLTHTERYMRFPAQKGCRGAVSFRVGRFGG
                     RFRFNARDEYFKRISMQGVQVLVYREHDYRCGSPPQQPPPCEYDLELEAPDPDAGLVV
                     QAKKTPEGRVDQRAVAFRALDTGLAVHVINASIAVPEAFEASEDLTSASVDGDAAGPW
                     LSGDLSYVGPPATEGEVDDCGANQASSGIATGDYTAHFDSIGDVTVAATGLSATVRRR
                     I"
     gene            3996..4145
                     /locus_tag="VEV43_07320"
     CDS             3996..4145
                     /locus_tag="VEV43_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23365.1"
                     /translation="MATKAICVSCERTVFLEANDTGSCPVCSSPVIPALADTSPASLE
                     GYYLG"
     gene            complement(4147..>4500)
                     /locus_tag="VEV43_07325"
     CDS             complement(4147..>4500)
                     /locus_tag="VEV43_07325"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HYP23366.1"
                     /translation="FLLVVPFSQGFVKVTSSQKCVFFAAFLCTTAAALLLIAPTTYHR
                     LRWRQHDKEQMLATANKMVIAGMGLLALAMCCVVYFITSFLYGGPAPWLTAVAGGAFA
                     FFWFGLPLYRKAKEA"
BASE COUNT          669 a         1603 c         1534 g          694 t
ORIGIN      
        1 gtcgcggccc ccagcccgac gacgccgacg accgtcaccg tcactgcgat cgacccgccg
       61 gcgggatacg tctcctcttc gaggtgcgtc cgccatccga ggaggtagag ggcgtgcagc
      121 ggcgccgagc acgcgagcag gaccgcagcc gcccaccggc ccatcccgcc gccgagcacc
      181 gacatcgccg cccccagcat cgtgacgacg aaggcgtagc cgaggaaggt cccgatctcg
      241 gggaccccct tgccgcgggg agtcccggct ccggcgtcca gcgacgcggc ccgccgcacg
      301 aaccacagcg agggcacgaa cccggcgatc gcacccaccc acacgaacgg cgcgtacctg
      361 atcgcaaccc cggcgaagaa caacgcggcg ctcggcagga gcgcgatagc ggcaccggcg
      421 gccagccgga gacacctcac ccatgcgacc gcgagggggg cgcggcgtgc gtccttcagc
      481 cacagcgtca acgcgagcgc cccgctgcgg accactatcg acagcgcgac ggcggccgcg
      541 aaccacggcc acccgcgccc gtgcgcgagg gcttgcttga cgtcgaagaa gacgtcgagg
      601 gggaagacgg cggcgctggc ggcggccgca ccgtctccgg ggaacgcacc ggttaggacg
      661 aggacgccgg cgatcaagcc ctcggccgcg aacggtgcca tcgcgagcgg agcctctcgc
      721 agcacgcgca gccccgccac gatcgttcgc acaggcgtgg agactaacta tgctcccgtt
      781 ccgtggcagc ggccgaaccc gaactccccg tgatcgagct tcccgctcgg tcgcggcgcg
      841 ctggctggtc gggacgggac tggctcgcgc tgagcgcggt gacgatcggg agcgcgctgc
      901 tgcgcttgac gcggctcgcc gaccccaagc acctgatctt cgacgaggtc tactacgccc
      961 gcgacgcatg ctggtacgtg tacgcggccc cctcggtgtg cggcatcgac cacgagagca
     1021 cgcccgtcca ccccccgatg tccaagtggc tgatcgcagt cgggatcagg ctcttcggct
     1081 acgactcgtt cggctggcgg ttcaccgcgg cgatcgccgg ggtcgtcacc gtggcgctcc
     1141 tctacctgct ggcccgcaag gtcctgggct cgaccctcgg ggcgacgctg gccgccgggc
     1201 tcctcggcgt cgacttcctg cacttcgtcc agtcgcgcac gtcgatgctg gacatcttca
     1261 tcccgatgtt cggcgtcgcg gcgatgctgt tcgtcgcgta cgaccgcgac cggatgctcg
     1321 cgctgctcga ccgccccccc gacgagcggc cgcacggact gctcgaccgg ccctggaggc
     1381 tcgccgcggg cgcggccggg gcgggtgctg tcgcgtcgaa gtggtccggg ggcatggtct
     1441 tgatgatcgt gatcctgctc acgttcgcgt gggagatcgg gacgcgccgg cgcgacggcc
     1501 acccgcgtcc gttgcgccgg ttcgcgcgcg aggagctccc ctcgatcgcg gtctggctcg
     1561 tgatccttcc ggtcgcgctc tacggcctca cgtacgtcgg ccgcatcggc gggtcgtaca
     1621 ccgcgctccc ctggaccgcc gacgcgtggg tgaacaacgt ctggaaccag cagttcgaga
     1681 tggcgaactt ccacgacgac ctcgacgcga cccactcgta ccagtcgccg tggtggtcgt
     1741 ggatcctgct gaagcggccg gtctcgtacg cctattgcgg gggcgactcg tgcgatcccc
     1801 ccgcgccgaa cggcgaggtg aaggaggtgc tcgcaaccgg cagcccgttc gtgtggggcg
     1861 cgtcgatcct cgccgtgctg ttcgcgctgt ggcggtgggt gaggaaatgg gagtggtgga
     1921 agcccaagcg tgacgcccgg acgctggtcg gagggaccga gggcttcgtc gtcgcggggt
     1981 tcctgctgac ctacctgccg tggatcgcgc tcacgtggaa ccgtgaggcg gtcttcatct
     2041 tctacatcct gccgacgatc ccgttcatgt gcctggcgct cgcgtgggtc gtcacgcgga
     2101 tcgggtggac gtgggaggcg aaggcggccg tcgcgctgtt ctcgctcggc gcggtcgggc
     2161 tcttcgtctt cttctacccg atcctcgcga acgtctccat ccccgagcac cactggcgct
     2221 cccgcatcca ggcgttcgac gactgcgagg cccccgagcc cgaggagtcg acgtcgaccg
     2281 tgacgtcgac ggtcaacggc aagaaggtcg agaggatcgt cacgagcacc ggcgacggcg
     2341 gcggcccgcc ggccgggtgg tgctggatct agggcgcctc ttcgacggac acgatctcga
     2401 cctcgaagac cagcgtcgcg ttcggcggga tgtccggcgg gtacccgctt tcgccgtagc
     2461 cgagctcgga cgggatcgtc agcttgcgac gcccgccctc cttcatgccg gggatgccct
     2521 cgacccagcc ttcgatgagg ttcccggact cgagcgcgaa cttcacgggg tcggcgccga
     2581 cgttcgagtc gaactcggtc ccgtcgtcca gcgtgcccgt gtagaagacc tcgatgacgt
     2641 cgccgcgggc gacctcggtg ccgtcgccgc actcgagctc ctcgtactcg aggcccgagt
     2701 cgagctcgac gaccgctccc tcctcgcatc cgtcggagcc gccccctccc gcgtcggatc
     2761 ccgacggcgt gtcgtcgccg cacgcggcgc cgaggagcaa caggagcgcg atgagtaacg
     2821 tccggaaggt cttcatgggc gcaccttaaa tccgccgccg cacggtcgcg ctcaggccgg
     2881 tggccgcgac cgtgacgtcg ccgatcgagt cgaagtgcgc ggtgtagtca ccggtcgcga
     2941 tccccgagga cgcctggttc gcgccgcagt cgtcgacctc gccctcggtc gccggcggcc
     3001 ccacgtagct gaggtcaccg ctcagccacg ggccggccgc gtcgccgtcg acgctcgcgg
     3061 acgtgaggtc ctcggatgct tcgaacgcct cggggacggc gatcgacgcg ttgatcacgt
     3121 ggacggccaa gcccgtgtcg agcgcccgga aggcgacggc cctctggtcg accctgccct
     3181 cgggagtctt cttggcctgc acgacgaggc cggcgtcggg gtccggggcc tccagctcga
     3241 ggtcgtattc gcacggcggt ggctgctgcg gaggcgaccc gcaccggtag tcgtgctcgc
     3301 ggtacacgag cacctggacg ccctgcatcg agatccgctt gaagtactcg tcgcgcgcgt
     3361 tgaagcggaa ccggcccccg aacctcccga cgcggaagga cactgcgccg cggcatccct
     3421 tctgcgcggg gaagcgcatg tacctctcgg tgtgcgtcag cttcatgtcg acgaagccgt
     3481 tgttccccat ccccctgcgg gtgtcgagcg acacgggccg gaggtccgag gccatcctca
     3541 tcgcgccgcg ggggagctcc cagcggaact cgctcctctg ggactgcgtg tcctgggccc
     3601 ccgtccggat gaagccgagc accagcgacg tcttcgcccg agggttcttc ttgtaggcga
     3661 ggacggtcca gcggtagccc ttgtagcctg gagggacgag ctggatgaac atctggttcg
     3721 agccgccatc tctcggctgt tcgaacaggg cccgggcttc gtcgatcgac cggggcagcg
     3781 gcgtcgccga tgcagggggc gtcaggccgg tcgcaaccac tacggccgcg atcgcgacca
     3841 ccgctcgtct catctccacc tctctcgtcc gtcgagcccc gatcagtatg ccttttcgga
     3901 tgaattcggg ctcgacacgg cggcgcgaaa cgggcacaat cgccgaacgg gttgagacgc
     3961 agcctcgacc gaccgtcacg tttccggagg gggccatggc gaccaaagcc atctgcgtga
     4021 gctgcgagcg aaccgtcttc ctggaggcga acgacaccgg gtcctgcccc gtgtgctcct
     4081 cgcccgtgat ccccgcgctg gccgacacct ctccggcgag cctcgagggc tactacctgg
     4141 gctaggtcag gcctctttcg ccttccggta cagcgggagc ccgaaccaga agaacgcgaa
     4201 cgcgcccccc gccacggcgg tgagccaggg ggccggtccc ccgtacaaga agctcgtgat
     4261 gaagtagacg acgcagcaca tcgcgagcgc cagaagcccc atcccggcga tgaccatctt
     4321 gttggccgtc gcgagcatct gctccttgtc gtgctgtcgc cagcgcaggc ggtgataggt
     4381 cgtcggggcg atcagcagga gcgccgcggc cgtcgtgcag aggaacgccg cgaagaagac
     4441 gcacttctgt gaggacgtca ccttcacgaa accctgactg aacgggacga cgagcaggaa
//
LOCUS       DASQDO010000258         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_882818, whole genome shotgun sequence.
ACCESSION   DASQDO010000258 DASQDO010000000
VERSION     DASQDO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882818"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..2158
                     /locus_tag="VEV43_07330"
     CDS             221..2158
                     /locus_tag="VEV43_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYP23367.1"
                     /translation="MSDFVWEPTAEHLERAAVTRLMRRHGIETYQELVRRSSDDLEWF
                     WNAVVEDVGIDFFRPYDTLLETPKGPQWPRWFTGGLVNLAYNCVDRHAASARAGDRAL
                     VWEGEDGDVRTLTYADLGEAVNRAANGLAELGIGEGDAVGVYMPMVPEAVVAMYACAK
                     LGAIYLPIFSGFGAPAIATRLQDAGATALVTADAFYRRGSKVPMKATADEAVAASPTV
                     EKVVVYRRFPDDEIASGDKDVDWAELCGRQSPERASAELDPEAPLMIAYTSGTTGRPK
                     GSLHVHGGFLVKIASEAAYQFDMAPGEVLYWVTDMGWIMGPFEMVGGHSNGACVLMYE
                     GAPNYPDAGRVWQMCERHGVTVLGISPTLVRALMPAGEEPVKAADLSKLRLLGSTGEP
                     WNPEPYRWFSDVVGGGRCPIINISGGTEIGACFLGQAPVIPTKACSLGAPWLGMAIDV
                     FGPDGKPVRGEVGELVCTQPWPAQTRGFWNNPQRYLDAYWSRWPDVWVHGDWASVDDD
                     GYWFLHGRSDDTLNIAGKRIGPAEFESAAVDHPAVVECAAVGVPDEVKGETVWCFCIL
                     RPGEDPSDELAAEVKATIANELGKAFAPAAIRFVEELPKTRSAKILRRAIRAQVLGED
                     PGDLSSLENPSALEAVKKSLA"
     gene            2155..>2395
                     /locus_tag="VEV43_07335"
     CDS             2155..>2395
                     /locus_tag="VEV43_07335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYP23368.1"
                     /translation="MTTLWLVRHGVTSHTGHKLSGWMPDVHLTDEGREQADAAAELLS
                     SMNLEAVYSSPIDRTRETAAAMAARHGLPVRTRRTI"
BASE COUNT          366 a          809 c          863 g          357 t
ORIGIN      
        1 tccgcggcca gcaggctgct tcgctcacgc ctcccgcggg agggctcgag tccttcgacg
       61 tgaggttcgg cgagaagagc ggcaagaagc cgttcgtcta cttctgcttc ctgcacgaga
      121 tggacgggct cataacggtc aactgacccc tgaccagtgg gaagaggcgg cccttcgggg
      181 ccgcctcttc gcgtggtggc cccgcgatag cctgcgggtg atgagcgact tcgtgtggga
      241 accgactgcc gagcacttgg agcgcgcggc cgtgacgcgc ttgatgcggc gtcacgggat
      301 cgagacgtat caggagctcg tccgccggtc ctccgacgac ctcgagtggt tctggaacgc
      361 ggtcgtcgag gacgtcggca tcgacttctt ccgtccctac gacaccctgc tcgagacacc
      421 gaaaggcccg cagtggccgc gctggttcac cggcgggctc gtcaacctcg cctacaactg
      481 cgtcgaccgt cacgcggcct ctgcgcgcgc cggcgaccgc gctctcgtgt gggagggcga
      541 ggacggagac gtccggacgc tgacgtacgc ggacctcggc gaggccgtga accgcgccgc
      601 caacggtctt gccgagctcg ggatcggcga gggcgatgcc gtcggggtct acatgccgat
      661 ggtccccgaa gccgtcgtgg cgatgtacgc gtgcgcgaag ctcggcgcga tctacctgcc
      721 gatcttctcg ggcttcgggg cccccgcgat cgcgacccgg ctgcaggatg cgggggccac
      781 ggcgctcgtg accgccgacg cgttctaccg gcgaggctcg aaggtcccga tgaaggcgac
      841 ggcggacgag gccgtggccg cgtcgccgac ggtcgagaag gtcgtcgtct accgccgctt
      901 cccggacgac gagatcgcgt ccggggacaa agacgtcgac tgggccgagc tgtgcgggcg
      961 gcaatcgccc gagcgagcgt ccgccgagct cgaccccgag gccccgctga tgatcgccta
     1021 cacctccggc acgaccggcc gcccgaaggg gagtctccac gtgcacggcg gcttcctcgt
     1081 gaagatcgcg tccgaggccg cgtaccagtt cgacatggct cccggcgagg tcctgtattg
     1141 ggtgaccgac atgggctgga tcatgggccc gttcgagatg gtcggcgggc actcgaacgg
     1201 cgcgtgcgtg ctgatgtacg agggcgcgcc gaactacccc gacgccgggc gcgtatggca
     1261 gatgtgcgaa cgccacggcg tcaccgtgct cgggatctcg ccgacgctgg tgcgcgctct
     1321 catgccggcg ggcgaggagc ccgtgaaggc agcggacctc tcgaagctgc gcctgctggg
     1381 ctcgaccggc gagccgtgga accccgagcc gtaccgctgg ttctccgacg tcgtcggtgg
     1441 gggccgctgc ccgatcatca acatctcagg cggcaccgag atcggtgcgt gcttcctggg
     1501 ccaggccccc gtgatcccga cgaaggcgtg ctctctcgga gcgccgtggc tcgggatggc
     1561 gatcgacgtc ttcgggccgg acgggaaacc cgtgcgcggc gaggtcggcg agctcgtgtg
     1621 cacccagccg tggcccgcgc agacgcgcgg cttctggaac aacccgcagc ggtacctcga
     1681 cgcgtactgg tcgcgatggc cggacgtgtg ggtccacgga gactgggctt ccgtcgacga
     1741 cgacgggtac tggttcctgc acggccggtc ggacgacacc ttgaacatcg cgggcaagag
     1801 gatcggcccg gccgagttcg agtccgccgc ggtcgaccat ccggcggtcg tcgagtgcgc
     1861 ggccgtggga gtccccgacg aggtcaaggg cgagaccgtg tggtgcttct gcatcctgcg
     1921 acccggcgag gacccgagcg acgagcttgc ggccgaggtc aaggcgacga tcgcgaacga
     1981 gctcggcaag gcgttcgcgc cggcggcgat ccgcttcgtc gaggagctgc ccaagacacg
     2041 ctccgccaag atcctgcggc gcgcgatccg cgcgcaggta ttgggagagg accccggcga
     2101 cctctcgtcg ctcgagaacc cgagcgctct cgaagcggtg aagaagtcgc tcgcatgacg
     2161 acactctggc tcgtcaggca cggcgtcacc tcgcacaccg ggcacaagct gtcggggtgg
     2221 atgccggacg tccacctgac cgacgagggc cgcgaacagg cggacgctgc ggcggagctc
     2281 ctgtcgtcga tgaacctcga agccgtgtac tcgagcccga tcgacaggac gcgcgagacc
     2341 gcggccgcga tggccgcacg ccacggcctt ccggtgcgca cgcggcggac catcg
//
LOCUS       DASQDO010000259         5402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1127662, whole genome shotgun sequence.
ACCESSION   DASQDO010000259 DASQDO010000000
VERSION     DASQDO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127662"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VEV43_07340"
     CDS             <1..474
                     /locus_tag="VEV43_07340"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23369.1"
                     /translation="VVLDQLGITAYPKTSGATGMQIMVPLDGTVDYDHVRGFVGTVSD
                     LVHAADPETTTIEWEVSKRSDKVFLDVNMNREGANIAAAYSARPEPGATVSTPFEWDE
                     LDEVEPSLFSIETIFDRIASAGDPFLPVARGPGQSLDEAIEQLDAKPRRARMIKR"
     gene            471..1199
                     /locus_tag="VEV43_07345"
     CDS             471..1199
                     /locus_tag="VEV43_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYP23370.1"
                     /translation="MTLFVGTSGWAYREWKPDFYPADVKQKEWLRFYSSTLGACEINA
                     TFYRLQSTETMERWRDETSDGFLFSLKMHRRITHSRSIAPDEPRIDLLKTFFDHAARL
                     GPKLGVVLLQFPPHRVRDDAALDALIAALPPDRRYAFEFRNETWDHDEVRGRVAAARG
                     TICLSNTDGKAPDSLPPGELAYVRLRTDEYEESERKAWTELLLAEGERRDVFAFAKHE
                     GIPAGNPYGGVGLAKWLAETAAGT"
     gene            complement(1236..2963)
                     /locus_tag="VEV43_07350"
     CDS             complement(1236..2963)
                     /locus_tag="VEV43_07350"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HYP23371.1"
                     /translation="MKRIAVVLALLALAAAAVAPASAGKRGSSLRRGLDLSLDAPVHI
                     PDHGAHLDEDTAALSSVTTDRLSRSLTSGRISPAKFALERAEATLDGDFDGHLSLVLT
                     DLALRVRELSGADRERAQAILARPTDPDTDANINRVSYRDADVNASCSAHFCIHWTST
                     GRHAVAQTDANSNTTPDYVETVQVEMENVWAAIVDGHGFKAPKSDLTSENTVSGPDAG
                     KLDVYLVEAGSDGVYGYCTTDDPNLLDPSLGYEYFDGSAYCALDDDFAPSQFGGAPAE
                     ESLRVTAAHEFFHAVQLSYAAGHDTWMSEGTATLMEDVVHDDVDDNYQYLSVSAMRTL
                     NGRTTVPLDAGIAPYHYGAWLWWRFLVELEETGGLPIIRETWEYAALDGPGADGLVST
                     FAIRKALADHNVSFAGAKAFFSVVNYLPEGFYEEGAAYRAEMNGKRPTPMATFTLGGT
                     KKGTGLRVAKLDHLSAGYVVFKPGAGVTKLRAGVDLPAGATSPSAAVLVLYTDGTVER
                     ASIPVNGTGVGALKGIPFRKGEVASVVLVLDNASTRFKACFQRSTPWTCSGIPRDDAL
                     PFQLSGTAS"
     gene            3130..4623
                     /gene="ligD"
                     /locus_tag="VEV43_07355"
     CDS             3130..4623
                     /gene="ligD"
                     /locus_tag="VEV43_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYP23372.1"
                     /translation="MPETRDYARKRRFDETPEPPPAVSGDVDVAKALPGGTFVIHQHH
                     ARRLHFDLRLEMYNGDVPVLVSWAVPKNLPLRKGKPHLAVHVEDHPFEYGSFSGTIPA
                     GNYGAGEVRIFDHGEFELLEQERGKLTFRLSGERMKGVWHMALTDKTEAKKDWLVFLS
                     KDERPEPDPLPELTPMLATLVEEPFDREGWIFELKWDGVRALGVCSDETALISRNRND
                     ITVCYPELHDLHTRLVCLDAIVDGEIVAMSAGRPSFEKLQSRINLQNPRDIERMSKQI
                     PVTYVVYDLLFLDGRSTMDLPLERRKELLDELVVPSEKVQVSHCTPGEGALLFEFAKT
                     NKIEGIVAKKGSSTYRPGKRSRDWLKIKTVWEADVVVGGWSKGEGNRSSTFGSLIVGA
                     YDSDGLHFLGSVGTGFSDKALESLVPSLQSLASPECPFVEDPVGLSSGRWGKPLKDPQ
                     WVRPELVAKVEFRELTSGNRLRAASFKGLRADKDASDCRLEDLVPEG"
     gene            4632..5258
                     /locus_tag="VEV43_07360"
     CDS             4632..5258
                     /locus_tag="VEV43_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYP23373.1"
                     /translation="MAVGRGQKLIAGIYSRAAHRLYDPLVVHGSFKLLSGGRLHDLVA
                     EQGRRAVSAADGGPILDVPVGTAYFTKAVARAHPGLVVGVDIAEGMVQESRDLAAREG
                     LSNLSVVQASIHELPFPDGTFPAILCTNGLQVMPNLEGAVGELARVLAPGGTLFVSVV
                     GVPLRSKSLPTAFATRNVSDALRGVGLRRTRFLRLRLATIICARKVMP"
BASE COUNT          875 a         1756 c         1892 g          879 t
ORIGIN      
        1 gtcgtcctcg accagctcgg gatcaccgcg tatccgaaga cctccggtgc gaccgggatg
       61 cagatcatgg tcccgctcga cgggacggtc gactacgacc acgtgcgcgg cttcgtcggc
      121 acggtctccg acctcgtgca cgcggccgac cccgagacga cgacgatcga gtgggaggtc
      181 agcaagcgct cggacaaggt ctttctggac gtgaacatga accgcgaggg ggcgaacatc
      241 gcggccgcgt actcggcccg gccggagccg ggcgcgacgg tgtcgacgcc gttcgagtgg
      301 gacgagctcg acgaggtaga gccgtcgctg ttctcgatcg agacgatctt cgaccggata
      361 gcgagcgccg gggacccgtt cctgccggta gcacgggggc cgggccagtc gctggacgag
      421 gcgatagagc agctcgacgc gaagccgcgc agggcccgga tgatcaagcg atgacactgt
      481 tcgtcgggac ttcgggctgg gcctaccgcg agtggaagcc cgacttctat cccgccgacg
      541 tcaagcagaa ggagtggctc cgcttctact cgtcgacgct cggcgcctgc gagatcaacg
      601 cgacgttcta ccggctccag tcgaccgaga cgatggagcg gtggcgcgac gagacgtcgg
      661 acggcttctt gttctcgctc aagatgcacc gcaggatcac gcactcgcgg tcgatcgcac
      721 ccgacgagcc gaggatcgac cttctcaaga cgttcttcga ccacgcggcg cggctcggcc
      781 ccaagctcgg cgtcgtgctc ttgcagttcc cgccgcatcg cgtccgcgac gacgccgcgc
      841 tcgacgcgtt gatcgccgcg ctcccgccgg accgccgcta cgcgttcgag ttccgcaacg
      901 agacgtggga ccacgacgag gtcaggggcc gggtcgcggc cgcccgcggc acgatctgtc
      961 tgtcgaacac cgacgggaag gcgccggact cgctgcctcc gggcgagctc gcctacgtcc
     1021 ggctgcggac cgacgagtac gaggaatcgg agcgcaaggc gtggaccgag ctgctgctgg
     1081 ccgagggcga gcgccgggac gtcttcgcct tcgcgaagca cgagggcatc ccggccggga
     1141 atccctacgg cggcgtcggc cttgcgaagt ggttggcgga aaccgccgca gggacctagc
     1201 cctagatccc tgcggcggct ccttcagact cttggctagc tcgccgtccc cgagagctgg
     1261 aacggcagcg cgtcgtccct cgggatgccc gagcaagtcc acggcgtgct gcgctggaag
     1321 caggccttga agcgcgtgct cgcgttgtcg aggacgagca cgacgctggc gacctcaccc
     1381 ttgcggaacg ggatcccctt cagcgccccc accccggtgc cgttcacggg gatcgacgcc
     1441 ctctcgaccg tcccgtcggt gtagaggacg aggacggcag cgctcggcga cgtcgcgccg
     1501 gccggcaggt cgacgcccgc ccgcagcttg gtcacgccgg cacccggctt gaagaccacg
     1561 tagccggcgc tgagatggtc gagcttcgcg acgcgcagcc ccgtgccctt cttggtcccg
     1621 ccgagcgtga acgtcgccat gggcgtcggc cgtttcccgt tcatctccgc gcggtacgcc
     1681 gcgccctcct cgtagaagcc ctcgggcagg tagttgacga ccgagaagaa cgccttggca
     1741 ccggcgaacg acacgttgtg gtcggcaagc gccttcctga tcgcgaacgt cgagacgagg
     1801 ccgtcggcac ccgggccgtc gagcgccgcg tactcccacg tctcgcggat gatcgggagc
     1861 ccgccggtct cctcgagctc gacgaggaac ctccaccaca accaggcgcc gtagtgatac
     1921 ggcgcgatcc cggcgtcgag cggcaccgtc gtccgtccgt tgagcgtgcg catcgcgctg
     1981 acgctcaggt actggtagtt gtcgtcgacg tcgtcgtgaa cgacgtcctc catcagcgtc
     2041 gccgtgccct cggacatcca cgtgtcgtgg ccggcggcgt aggagagctg gacggcgtgg
     2101 aagaactcgt gggcagcggt gaccctcagc gactcctcgg cgggcgcgcc gccgaactgg
     2161 ctcggcgcga agtcgtcgtc gagcgcgcag tacgccgatc cgtcgaagta ctcgtagccg
     2221 agcgacgggt ccaagaggtt cgggtcgtcg gtcgtgcagt atccgtagac gccgtcgctg
     2281 ccggcctcca cgagatagac gtcgagcttg ccggcgtccg gcccgctgac cgtgttctcg
     2341 gacgtcaggt cggacttcgg agccttgaac ccatggccgt cgacgatcgc cgcccacacg
     2401 ttctccatct cgacctgcac ggtctcgacg tagtccggcg tggtgttcga gttggcgtcc
     2461 gtctgggcga cggcatggcg ccccgtgctc gtccagtgga tgcagaagtg cgcgctgcac
     2521 gacgcattga cgtcggcgtc ccggtacgag acgcggttga tgttggcgtc ggtgtccggg
     2581 tcggtaggcc gggcgaggat cgcctgcgca cgctcgcggt cggcgccgct cagctcacgg
     2641 acacgcagcg ctaggtcggt gaggacgagg ctgaggtggc cgtcgaagtc cccgtcgagg
     2701 gttgcctccg cgcgctccag agcgaacttc gccggcgaga tccggcccga cgtcaacgag
     2761 cgggaaaggc ggtcggtcgt gaccgagctg agggccgcgg tgtcctcgtc gaggtgcgcc
     2821 ccgtggtccg ggatgtggac cggcgcgtcg agcgacaggt cgagccccct gcggagggag
     2881 gaacccctct tgcccgcgga cgccggcgcg accgccgccg cagcgagggc gaggagtgca
     2941 agtacgacgg cgattctctt catgcgcgga ggatcctccc ggtcggatgg gcagccacga
     3001 ggagtcaatc ggaaaggggg gccggaatct gtacgtaact agtgaagtag gccgtcttcg
     3061 gctgatccga gcaaaacggg accaaccggg cgcgcgcccg ccggcgtcgt gctgtgacaa
     3121 ccttggacga tgcccgagac ccgtgactac gcgcgcaagc gccgcttcga cgagacgccc
     3181 gagccgcctc ccgccgtgtc gggcgacgtc gacgtcgcaa aggcccttcc gggcgggacg
     3241 ttcgtgatcc atcagcacca cgcgcgccgg ctccacttcg acctcaggct cgagatgtac
     3301 aacggcgacg tgcccgtgct cgtgtcgtgg gccgtgccga agaacctgcc gctgcgcaag
     3361 ggcaagccgc acctggccgt ccacgtcgag gaccacccgt tcgagtacgg ctcgttctcc
     3421 gggacgatcc cggcgggcaa ctacggagcc ggcgaggtcc gcatcttcga ccacggcgag
     3481 ttcgagctcc tcgagcagga gcgcggcaag ctcacgttcc ggctgtcggg cgagcggatg
     3541 aagggcgtct ggcacatggc cctcaccgac aagaccgagg cgaagaagga ctggctggtg
     3601 ttcctgagca aggacgagcg gccggagccc gacccgctgc ccgagctcac gccgatgctc
     3661 gcgacgctgg tcgaggagcc gttcgaccgc gaggggtgga tcttcgagct gaagtgggac
     3721 ggggtccgcg ctctcggtgt gtgctccgac gagaccgcgc tgatctcgcg caaccgcaac
     3781 gacatcaccg tctgctatcc ggagctgcac gacctgcaca cgcggctcgt gtgcctcgac
     3841 gcgatcgtcg acggcgagat cgtcgcgatg tcggcgggcc ggccctcgtt cgagaagctc
     3901 cagtcccgca tcaacctgca gaacccgcgc gacatcgagc ggatgtcgaa gcagatcccg
     3961 gtgacatacg tcgtgtacga cctgctgttc ctcgacggtc gctcgacgat ggacctgccg
     4021 ttggagcggc gcaaggagct gctggacgag ctcgtcgtcc cgtcggagaa ggtgcaggtc
     4081 tcgcactgca cgcccggtga gggagcgctt ctgttcgagt tcgccaagac gaacaagatc
     4141 gagggcatcg tcgcgaagaa ggggtcgagc acgtaccgcc ccggcaagcg gagccgcgac
     4201 tggctgaaga tcaagacggt gtgggaagcc gacgtggtgg tcgggggctg gtcgaagggc
     4261 gagggcaacc gttcgtcgac gttcggatcg ctgatagtcg gggcctacga ctcggacgga
     4321 ttgcacttcc tgggctcggt ggggactggt ttctcggaca aggcgctgga gtcactggtg
     4381 ccgtcgttgc agtcgttggc ctcgccggag tgcccgttcg tggaggaccc ggtcgggctg
     4441 tcgtcgggcc ggtgggggaa gccgctgaag gacccgcagt gggttcggcc cgagctggtg
     4501 gcgaaggtcg agttccgtga gctgacgtcg ggcaaccgcc ttcgtgcggc gtcgttcaag
     4561 ggcctgcgtg cagacaagga cgcgtcggac tgccggctag aggacctggt cccggagggc
     4621 taggttcgga cgtggcggtg ggtcgcggtc agaagttgat agccggaatc tattcgcgcg
     4681 ccgcgcacag gttgtacgac ccgttggttg tgcacggatc gttcaagcta ttgagcggtg
     4741 gccgtctgca cgatctggta gctgaacagg gccgccgtgc cgtttcggct gcggacggcg
     4801 gcccgatcct ggacgtaccg gtcgggacgg cctacttcac gaaggcggta gcccgtgccc
     4861 acccaggctt ggtggtcggt gtggacatag ccgagggcat ggtgcaggag tctcgtgacc
     4921 tagctgctcg cgagggtctc tcgaacctgt cagttgtcca ggcgagcatc cacgagttgc
     4981 ccttccccga cgggaccttc ccagcaatcc tgtgcacgaa cgggctgcag gtgatgccga
     5041 acctagaggg ggccgtgggg gagctggccc gtgtgctcgc tccgggcggc acgctgttcg
     5101 tctcggtagt cggggtaccc ttgcggagca agtcgcttcc gacggctttc gcgacgagga
     5161 atgtctcgga cgctctcagg ggggtcgggc tgcgacgaac aagattcctc cggttgcgac
     5221 tcgcaacgat catttgcgcc aggaaagtca tgccataagg tcatacagcc caaagtcaga
     5281 gtctttacag atcgccgctg tcacgcaccg ggaatcattg gccaatccgt acacgcttca
     5341 cgctcgccac agagccgatt gcgcgctcac gagtctcggc atctaaaaca ttcaacacca
     5401 tg
//
LOCUS       DASQDO010000260         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_577292, whole genome shotgun sequence.
ACCESSION   DASQDO010000260 DASQDO010000000
VERSION     DASQDO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577292"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VEV43_07365"
     CDS             <1..307
                     /locus_tag="VEV43_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYP23374.1"
                     /translation="NRRRSSLRLRALRDKLALQVATPDQDRDAVDEGLLAAITALPDA
                     EREAFVLVAWDGLDPARAAVAAGCAPGTFRMRLHRARARLKRALGEDEATKTLEDSR"
     gene            304..1344
                     /locus_tag="VEV43_07370"
     CDS             304..1344
                     /locus_tag="VEV43_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23375.1"
                     /translation="MNDRTLTEMAAGVDPVSPGAWEGVAETQWGREMLEAIISSRPGR
                     ARWVMRTATAVAAVAVVTGIVVMTRSATQAPPPEREPAPIQVIAARQAPRLLVTAPGW
                     RLERAEDYGDDGGETNFRKGRLWVSINWYRAKFYDQYVNDRRRGAERSAEITIAGHDG
                     FLFRHDGNAPIGVTFYALWFDGSFAVELRTDVIPEESEFREIARSIERVDVDTWLAAM
                     PASVVTPDERQQAIERIVADMDVPPNVDLDALASRQVVTESLEYEVATAVACGWIERW
                     IDADRAGDEAGRREAAEAMAKAHDWDALASNGMWAQYVAGVAEEMADGAPAEGSGSVG
                     DRFRRDIGCGGG"
     gene            1590..2105
                     /locus_tag="VEV43_07375"
     CDS             1590..2105
                     /locus_tag="VEV43_07375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYP23376.1"
                     /translation="MPSTARFVTSEGTFTVRLMPDHAPTTVANFVDLATGKREWKDPR
                     DGQRKSEPLYDGTIFHRVIPDFMIQGGDPQGTGTGGPGYRFEDEVPPGGPKFDKPGLL
                     AMANAGPNTNGSQFFVTVAPTPWLTGKHTIFGEVTEGYDVVEKISKVQTGAQDKPVQD
                     VVLEKVEIEEQ"
     gene            2117..2881
                     /locus_tag="VEV43_07380"
     CDS             2117..2881
                     /locus_tag="VEV43_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP23377.1"
                     /translation="MAYEDSSRTVSVDGQSARLVELGSGEPIVVLHGWGGRVESMAPV
                     ISCFARDRRVLALDLPGFGESPAPASAWGTADYASFVRRVLGDAGVEEADFVGHSFGA
                     KTSLYLAATTGVVRRLVLAGSSGLRTPPSFQARLKRGVSRAGRAAKKLGPPGRALSAA
                     IYRRIASEDYRNAGPLRPTFVKVVNEDLTELLPRVRASTLLVWGTEDDAVPVAHARTM
                     EERIPDAGLVLFEGAGHFAYLDEPDRFCRVVRHFLR"
     gene            2878..4407
                     /gene="murF"
                     /locus_tag="VEV43_07385"
     CDS             2878..4407
                     /gene="murF"
                     /locus_tag="VEV43_07385"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HYP23378.1"
                     /translation="MNRADPFTGYSFVLRTIVYVVVPLFTAFQFVRLRRALHVFQLES
                     YKRPNYRRWLRANPGKARFLKPSTAKKPLVMTGRAWRILVTATLLSLFGVLVPSAVAH
                     LWLGGAPADLITWALVTVALFVWGPLLIPAADRLLAPVQRAINAGYVRRARRKLKDVA
                     PVVVGVTGSYGKTSTKFAVGGLLGPPGEVLVTPGSYNTTMGVVRAINENLESRHRFFV
                     VEMGARQRGDIAEIGRLVDHEIAVLTAIGTAHLESFGSREAIKAGKLEIVGTLRARGV
                     TVVNSDDATIRGLRDELAGADVVRYGLDPAGNPDVTATDVEVTRAGTEMTILDRRTGA
                     TLRAKTKLLGRHSIGHVLAGVAVALRAGRDLASLADAIAALTPVEHRLQLIEGAGGVT
                     VIDDAFNSNPEGAAAALDVLGSMPARQRIVVTPGIVELGDLQAAANEELGRAAAAVAD
                     VLIVVARLNRDALLAGAAGGRARVVAVASLDEATAELSKVVGPGDVVLFENDLPDQYE
                     G"
BASE COUNT          670 a         1493 c         1616 g          660 t
ORIGIN      
        1 gaacaggcgc aggtcctcgc tccggctgcg cgctctccgc gacaagctcg ccctacaggt
       61 ggcgacgccc gaccaggatc gggacgcggt cgacgagggc ttgctcgcgg cgataaccgc
      121 gctccccgac gcggagagag aggcgttcgt gctcgtggcg tgggacggcc tcgatcccgc
      181 ccgggccgcg gtcgccgccg gctgcgctcc ggggacgttc cggatgcgtc tccaccgtgc
      241 acgcgcgcgc ttgaagcgtg cgctcggcga agacgaagcg acgaagacct tggaggactc
      301 gagatgaacg accggacgtt gacggagatg gcagcagggg tcgaccccgt gagccccggc
      361 gcctgggagg gcgtcgccga aacgcagtgg gggcgcgaga tgctcgaagc gatcatctcg
      421 tcccggcccg gacgcgctcg ctgggtgatg cggaccgcta cggcagtcgc cgcggtagcc
      481 gtcgtcacag ggatcgtggt catgacccgt tccgcgacgc aggcgccgcc tcccgagcgg
      541 gagccggccc cgattcaggt catcgccgcc cgtcaggccc cacgccttct ggtcacggcc
      601 cccgggtgga ggttggagag agcagaggat tacggagacg acgggggcga gacgaacttc
      661 cggaaggggc ggctgtgggt ctcgatcaac tggtaccggg ccaagttcta cgaccagtac
      721 gtgaatgacc gccgacgagg ggcggaacgg tcggcggaga taacgatcgc cggtcacgac
      781 gggttcctct ttcgtcacga cggcaacgcg ccgatcggcg tgaccttcta cgcgctgtgg
      841 ttcgacgggt cgttcgccgt cgagctgcgg acggacgtga tccccgagga gtccgagttc
      901 cgggagatcg ctcggagcat cgaacgcgtc gacgtcgata cttggctcgc cgcgatgccg
      961 gcgagcgtcg tgacgcccga cgagcggcag caggcgatcg agaggatagt cgccgacatg
     1021 gacgtcccgc cgaacgtcga cctggacgcg ctcgcgtctc gccaggtcgt cacggagtca
     1081 ctcgagtacg aggtcgcgac cgccgtcgca tgcgggtgga tcgaaaggtg gatcgacgcc
     1141 gaccgggccg gtgacgaggc cgggcgccgt gaggcggccg aggcgatggc caaggcgcac
     1201 gactgggacg cgctcgcctc gaacgggatg tgggcccagt acgtcgccgg agtcgcggag
     1261 gagatggcgg acggagcgcc ggccgaggga tcggggtcgg tcggcgatcg tttccgccgc
     1321 gacatcgggt gcggcggggg gtagggctga ctagcggtgg tcggtcacca gggcgactgc
     1381 catgaggccc aggaagatcg ccgtcatcgc aaggatcatc ggcgcgtcca tctccttgtc
     1441 cctcccgtcg agcccatcgc gtagcctcct aagaacagtt tcggccgctc gcggataagt
     1501 ctctatagtc cgtttgggtc gtttcccctg tcgggatgac tatttcgtgg cgcttcgcgc
     1561 cgtttgcttg aaaacaacga ggaggaagca tgccgagcac agccaggttc gtcacgtccg
     1621 aggggacgtt caccgtccgc ctcatgcccg accacgcgcc gacgaccgtc gcgaacttcg
     1681 tagacctcgc gacgggcaag cgcgaatgga aggacccccg tgacgggcag cgcaagagcg
     1741 agcccctgta cgacgggacg atcttccacc gggtcatccc ggacttcatg atccagggtg
     1801 gcgacccgca ggggacgggc accggaggcc ccggctaccg cttcgaggac gaggtccctc
     1861 cgggcgggcc gaagttcgac aagcccggtc tgctcgcgat ggcgaacgcg gggccgaaca
     1921 cgaacgggtc gcagttcttc gtgacggtcg cgccgactcc ctggttgacc ggcaagcaca
     1981 cgatcttcgg cgaggtcacc gagggctacg acgtggtcga gaagatctcg aaggtccaga
     2041 cgggcgcgca ggacaagccg gtgcaggacg tcgtgctcga gaaggtcgag atcgaggagc
     2101 aatagcctgc acggccgtgg cctacgagga ttcttcccgg accgtctccg tcgacgggca
     2161 gagcgcgcgg ctggtcgagc tcggtagcgg cgagcccatc gtcgtcctgc acggatgggg
     2221 gggccgcgtc gaatcgatgg caccggtgat ctcctgcttc gcacgggatc gccgggtcct
     2281 cgcgctcgac ctccccgggt tcggcgagtc gcccgcgcct gcgagcgcgt gggggaccgc
     2341 ggactacgcg tcgttcgtgc ggcgggtgct cggcgacgcc ggtgtcgagg aggccgactt
     2401 cgtcgggcac tcgttcggcg cgaagacgtc gctgtatctc gcggccacga ccggtgtcgt
     2461 ccggcgcctc gtgctggcgg ggtcgtcggg gctgcgcacg ccgccgtcgt tccaggcgag
     2521 gctcaagcgt ggcgtctcgc gtgcggggcg cgcggcgaag aagctcggcc cccccggcag
     2581 ggcgttgagc gccgcgatct accgccggat cgcgtccgag gactaccgga acgcagggcc
     2641 gctgcgcccg acgttcgtga aggtcgtcaa cgaagacctg accgagctgc tgccgagggt
     2701 tcgtgcgtcg accctgctcg tgtgggggac cgaggacgac gcggtccccg tcgcgcacgc
     2761 gcggacgatg gaggagagga tcccggacgc ggggctcgtg ctgttcgagg gggccgggca
     2821 cttcgcgtac ctcgacgagc ccgaccggtt ctgccgggtc gtgcggcact ttctccgatg
     2881 aaccgggccg atccgttcac cggctactcg ttcgtccttc ggacgatcgt gtacgtcgtc
     2941 gtcccgctgt tcaccgcgtt ccagttcgtg cggcttcgcc gcgcgctcca cgtcttccag
     3001 ctcgagtcct acaagcggcc gaactacagg cggtggctgc gcgcgaaccc cggcaaggcg
     3061 aggttcctga agccgtcgac ggcgaagaag ccgctcgtga tgacgggccg cgcctggcgg
     3121 atcctcgtca ccgcgacctt gctgtcgttg ttcggcgtcc tcgtcccgtc ggcggtggcg
     3181 cacctctggc tcggaggcgc gccggcggac ctgatcacgt gggcactcgt gaccgtcgcg
     3241 ctgttcgtgt gggggccgtt gctgatcccc gccgccgacc ggctcctggc gccggtgcag
     3301 cgagcgatca acgcgggata cgtacggagg gctcgtcgca agctgaagga cgtcgcgccg
     3361 gtggtcgtcg gggtcaccgg ctcctacggc aagacctcta cgaagttcgc cgtcggcggc
     3421 ctcctcggcc cccccggcga ggtcctcgtg acgcccggct cctacaacac gacgatggga
     3481 gtcgtacggg cgatcaacga gaacctcgag tcccggcacc gcttcttcgt cgtagagatg
     3541 ggagcgcgtc agcgcggcga catcgccgag atcggccggc tcgtggacca cgagatcgca
     3601 gtgctgaccg cgatcgggac cgcgcacctc gagagcttcg gctcgcgcga ggcgatcaag
     3661 gccggcaagc tcgagatcgt cggcacgctc cgcgcccggg gagtgacggt cgtcaactcc
     3721 gacgacgcga cgatccgcgg cctgagggac gagctcgccg gagcagacgt cgtccgctac
     3781 ggcctcgacc cggcgggaaa ccccgacgtc accgccaccg acgtggaggt cacgcgggcc
     3841 gggaccgaga tgacgatcct cgaccggcgc accggcgcga ccctgcgggc gaagacgaaa
     3901 ctgctcggcc ggcactccat cgggcacgtg ctcgccgggg tcgccgtcgc gctccgcgcc
     3961 ggccgtgacc tcgcgtcgct cgccgacgcg atcgccgcgc tgacgccggt cgagcaccgc
     4021 ctccagctga tcgagggggc cgggggagtg accgtgatcg acgacgcgtt caacagcaac
     4081 ccggagggcg ccgccgcggc cctcgacgtg ctcggctcga tgccggcgcg ccagcggatc
     4141 gtcgtgacgc ccgggatcgt cgagctcggc gacctgcaag ccgccgcgaa cgaggagctg
     4201 ggtcgcgccg cggccgctgt cgccgacgtg ctgatcgtgg tcgcgcggct caatcgcgac
     4261 gcgctcctcg ccggagccgc agggggccgg gcccgtgtcg tcgcggtcgc ctccctcgac
     4321 gaggcgaccg cggagctctc gaaggtcgtc ggccccggcg acgtcgtgct gttcgagaac
     4381 gacctccccg accagtacga gggatgacgc acctcccgcc atagactctg cgcgatggc
//
LOCUS       DASQDO010000261         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_393903, whole genome shotgun sequence.
ACCESSION   DASQDO010000261 DASQDO010000000
VERSION     DASQDO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393903"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VEV43_07390"
     CDS             complement(<1..622)
                     /locus_tag="VEV43_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYP23379.1"
                     /translation="MPTPRRRALSDLAGAAGDLLVRSAGSAEVTGLAYDSSKVAPGDL
                     FFCVPGTRADGHDFARAAADAGAAALCVERDTCVDVPQVTVTDARRAMGRMASAFYGN
                     PADRLLLLGVTGTNGKTTTAFLVDAILRADGRTTGLVGTIETRVAGVARPGVRTTPES
                     LDLHALFAEMIDAGVDSVAMEVTSHALALHRVEGLCFAAAAFTNLTQ"
     gene            complement(632..2461)
                     /locus_tag="VEV43_07395"
     CDS             complement(632..2461)
                     /locus_tag="VEV43_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYP23380.1"
                     /translation="MAPRKSSSSSRGSNRRRSAPSSNKKSASRSPKRLGLTFILLTIT
                     FLAMGARLFMLQIVDAPAYAKLAAQQRDRTIEFPARRGAIFDRNGETLAISVDLKTIY
                     TDPVMVTDPARAAADLAPVLDQPYELLLQKLSGPMPGDDAPRFEYLMRQAPPEISKKV
                     AALGIDGVFMNDEAKRYYPGERLASHVLGFAGTDGVGLEGIEAQYDEILQGKAGRMTV
                     EQDLDGTVLPGGKIDMDRAEPGRALFLTIDKELQYFTQLTLADAAEKYHAEAGTAIVM
                     KPDTGEVLALANFPDFDPNEPGDYEPEMLQNRALNYVYEPGSAFKIVTAAAALDEKEV
                     TPRSQFVVADRMYYAGEEFNDSHPHPTETMTTTEIIEQSSNVGTIQIGLQLGGEKLDE
                     YVRRFGFGDPTGLDFPGESSGIVIDRDDWTAPTIATIPIGQGIAVTPLQMASAYSTLA
                     NGGVWVEPKLLASTMDGDGKVRPSPPAATRRVVSEKTARQMAKILTGVTRDGTGIEAQ
                     VPGYAVAGKTGTAQKPLPTGGYGNSYVASFAGFVPAQRPEIVVLVVLDEPSPIWGGST
                     AAPTFKVIAEFALRHLGVSPTGDAEKAAEAIEADQAGEEATYD"
     gene            complement(2462..>2908)
                     /locus_tag="VEV43_07400"
     CDS             complement(2462..>2908)
                     /locus_tag="VEV43_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HYP23381.1"
                     /translation="RAQEIRQAVRERAALRVVGDQRPMLKPHRRVSPLVVLAIVCVLG
                     TVALVLVERVMLAQSAFELTRLMERVEAADERRSELAFEVARRENPGRIEFYARTKLG
                     MVDPDPTKIQYIVADVAPRRSDSGGGSMRARMSGPASASGAWEDAP"
BASE COUNT          396 a         1024 c         1029 g          459 t
ORIGIN      
        1 cttgggtgag gttcgtgaac gcggcggccg cgaagcacag gccctcgacg cggtggagcg
       61 cgagcgcgtg gctcgtcacc tccatcgcga cggagtcgac gcccgcgtcg atcatctcgg
      121 cgaacagcgc gtgcaggtcc agggactcgg gggtcgtgcg gactccgggc cgggcgacgc
      181 ccgcgacgcg cgtctcgatc gtcccgacga gcccggtcgt gcgcccgtcg gcgcgcagga
      241 tcgcgtcgac gaggaacgcc gtcgtcgtct tgccgttcgt ccccgtcacg ccgagcagca
      301 acagccggtc cgcggggttc ccgtagaacg ccgacgccat ccggcccatc gcccggcgcg
      361 cgtcggtgac cgtcacctgc ggcacgtcca cgcacgtgtc gcgctccacg cacagggcgg
      421 ccgccccggc gtcggccgct gcccgtgcga agtcgtgccc gtcggcccgg gtcccgggga
      481 cgcagaagaa gaggtcaccc ggagcgacct tcgacgagtc gtaggcgagt cccgtcacct
      541 cggccgagcc ggcgcttcgg acgagcaggt cgcccgcggc gccggcgagg tcggacagcg
      601 ccctgcggcg gggggtgggc atcatcggat gctagtcgta ggtcgcttct tcgcccgcct
      661 ggtccgcctc gatggcctcc gccgccttct ccgcgtcgcc ggtgggcgac acgccgaggt
      721 gcctcagcgc gaactccgcg atgaccttga acgtgggggc cgccgtcgag ccaccccaga
      781 tggggctcgg ctcgtccagc acgacgagca cgacgatctc gggacgctgc gcgggcacga
      841 accctgcgaa cgacgcgacg tacgagttgc cgtatccgcc ggtcggcagt ggcttctgcg
      901 cggtcccggt cttgcctgcg accgcgtacc cggggacctg ggcctcgatc cccgtgccgt
      961 cgcgggtgac cccggtgagg atcttcgcca tctgccgcgc ggtcttctcg gagacgaccc
     1021 tgcgcgtcgc ggccgggggc gagggccgga ccttgccgtc gccgtccatc gtcgaggcga
     1081 gcagcttggg ctcgacccac acgccgccgt tcgcgagcgt cgagtacgcg gacgccatct
     1141 gcagcggcgt gaccgcgatc ccctgcccga tcgggatcgt cgcgatcgtc ggcgccgtcc
     1201 agtcgtcgcg gtcgatgacg atgcccgacg actcgcccgg gaagtcgagg ccggtggggt
     1261 cgccgaagcc gaacctgcgg acgtactcgt cgagcttctc gccgccgagc tgcaacccga
     1321 tctggatcgt cccgacgttc gacgactgct cgatgatctc ggtcgtcgtc atcgtctcgg
     1381 tggggtgcgg gtgcgagtcg ttgaactcct cgcccgcgta gtacatgcgg tcggcgacga
     1441 cgaactgaga cctcggcgtc acctccttct catcgagcgc ggcggcggcg gtgacgatct
     1501 tgaaggccga ccccggctcg tagacgtagt tcaaggcgcg gttctggagc atctcgggct
     1561 cgtagtcgcc cggctcgttc gggtcgaagt cggggaagtt cgccagcgcg agcacctctc
     1621 ccgtgtccgg cttcatcacg atcgcggtcc cggcttcggc gtggtacttc tcggccgcgt
     1681 ccgcgagcgt cagctgcgtg aagtactgca gctccttgtc gatcgtcagg aacagcgcgc
     1741 ggcccggctc ggctcggtcc atgtcgatct tgccgccggg gaggaccgtc ccgtcgaggt
     1801 cctgctcgac cgtcatacgg ccggccttgc cctggaggat ctcgtcgtac tgcgcctcga
     1861 tcccctcgag cccgacaccg tcggtcccgg cgaagcccag cacgtgcgac gcgagccgct
     1921 cgcccgggta gtagcgcttg gcctcgtcgt tcatgaatac gccgtcgatg ccgagcgcgg
     1981 cgaccttctt cgatatctcc ggcggcgcct gccgcatcag gtattcgaag cggggcgcgt
     2041 cgtcaccggg catggggccg ctgagcttct gcagcagcag ctcgtagggc tggtccagca
     2101 ccggcgcgag gtcggcggcg gcgcgtgccg gatcggtgac catgaccggg tcggtgtaga
     2161 tcgtcttcag gtcgaccgag atcgcgagcg tctcgccgtt gcggtcgaag atcgcgccgc
     2221 gccgcgcggg gaactcgatc gtccggtcgc gctgctgcgc ggcgagcttc gcgtacgcgg
     2281 gggcgtcgac gatctgcagc atgaacagcc gggcccccat ggcgaggaac gtgatcgtca
     2341 gcaggatgaa cgtgaggccg agccgcttcg gcgagcggga agcggatttc ttattggaag
     2401 aaggggccga ccggcgacgg ttcgaccccc ttgacgacga cgacgatttg cgcggagcca
     2461 tctagggcgc gtcctcccag gcccccgacg ccgacgccgg tcccgacatg cgagcgcgca
     2521 tcgagcctcc gccggagtcc gaccgccgcg gcgcgacgtc ggcgacgatg tactggatct
     2581 tcgtgggatc ggggtcgacc atgccgagct tcgtgcgcgc gtagaactcg atgcggccgg
     2641 ggttctcgcg ccgggcgacc tcgaaagcca gctcgctccg gcgctcgtcc gcggcctcga
     2701 cgcgctccat cagccgcgtg agctcgaacg cggactgggc gagcatcacg cgctcgacca
     2761 gcacgagcgc gacggtgccg agcacgcaca cgatcgcgag gacgacgagc ggcgacacgc
     2821 gccggtgcgg cttgagcatc ggccgctgat ccccgacgac tcgtagcgcg gcacgttcac
     2881 gtacggcttg cctgatctcc tgggcacg
//
LOCUS       DASQDO010000262         7305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_822107, whole genome shotgun sequence.
ACCESSION   DASQDO010000262 DASQDO010000000
VERSION     DASQDO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822107"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEV43_07405"
     CDS             <1..207
                     /locus_tag="VEV43_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP23382.1"
                     /translation="VETEFSLVRFGGDAEKAKQPYAGLDPLTAEDIADCIAWAVTRPP
                     HVDIDEIVVRPVAQARATEVARKP"
     gene            complement(204..1205)
                     /locus_tag="VEV43_07410"
     CDS             complement(204..1205)
                     /locus_tag="VEV43_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HYP23383.1"
                     /translation="MSPWLYIGFIAFVIAMLLVDLKLFHTEAHTPTTKESGTWVAIWV
                     GLALAFGVGLWFVQGSKDAGEYFAGYLIEYSLSVDNMFVFVVIFAYFKIPLSHQHQVL
                     FYGILGAMIFRGIFIALGTALVQNFDWVIPLFGLFLLYTAYKIAKGSAEHVNPEDNPV
                     LKFARKRFRTTHNLDGQKLFTMENGKRVATPLFIVLLFIETTDIFFAIDSIPAIFAVT
                     KEPFIVLTSNVFAILGLRALYFLLAGGMDRLHLLKYGLAIILGFVGVKMLLEGTEIVG
                     LTDHAIHVPIWLSLTVIIGVLTATAILSFKIPPKEQTDIEHRVSPDSDADEADHQRR"
     gene            1304..1741
                     /locus_tag="VEV43_07415"
     CDS             1304..1741
                     /locus_tag="VEV43_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23384.1"
                     /translation="MAVAAFAATLLAACSGDDSKGEFVAAADEACREADERISEIGRP
                     RVEEGIHDYVEQAQEISADLAADLRDLDPPPEDVARVEGMIDDLEAATDLLEPLAQAS
                     IDRDAEALEAIQQEVQRITDDVSEFAESYGFEVCGAKVLDPMR"
     gene            1738..2601
                     /locus_tag="VEV43_07420"
     CDS             1738..2601
                     /locus_tag="VEV43_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYP23385.1"
                     /translation="MSGIVHASAAAVAFGTLAVFAKLAYEDGAELLPLLATRFVVAGA
                     LLVLYHVVTRQRLWPGRRDAVRLLALGGLGYAAESALFFMGVERAPAAVVSLVFFTYP
                     MWTALLAFATRMERYSNRVAVSLVLGSAGVGLIFSVRLGSLLGPLFALLAAMSLAVYL
                     LVAQVVARDVAPSVAATWVSLGAAVAFGGGTLVSGDWLASDALDEAAALGIATSIAFV
                     LLYRAIALIGSARTAVASMLEPVATIVLAALLLDEAITARVALGAVLVVAALPILATA
                     PTVEPAPPAGP"
     gene            2880..4613
                     /locus_tag="VEV43_07425"
     CDS             2880..4613
                     /locus_tag="VEV43_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP23386.1"
                     /translation="MSRGGRLLRTVVVLAVLVAGGFGAAAALRGDPDQPEGPGGLPPP
                     DERTFETDDPVERACALDKPLLARVWRGYHPKHSEDVTFVPLEPNYSGAFDVTSHSGP
                     WDYLQNVPLVFYGPGVIAPEGRVRGPATLADVFPTVGAMAGVPLPEREGRVLHDALGE
                     TESAPRLVVTIMWDGVGRNVLERWPGRWPNLARMEREGTSYLTATVGSSPSITPATHS
                     TLGAGVYPRTHGVTAINIRTKNGDVRTAFNQRDPGDLKQTTFADEIDLALGNEPLVGM
                     LAWKSWHIGMMGHGTQTPGGDADQLAIIGGDERITGNPLYDSAPEYLENFGGLEEHAD
                     ELDLEDGEADGKWRGHGILELHDNPAWVEWETDAILAMLENEGYGDDEVTDFFFTNYK
                     PTDIVGHQYTMDSPEMGDVLEAQDAALGRLLDWLDANVSDYAVILSSDHGHTPSPERS
                     GAWPLLQGQLQEDIDAAFDVPKGKSLVEQANPVGPFLDKEVMNELGVTGAEVAEFLNG
                     YRIRDNWGGGELPEGFEDRGDENVIAAAWSRRQYGEVMRCAFGSEKPPASAGLRAEPE
                     AASAGPATDTE"
     gene            4629..6281
                     /locus_tag="VEV43_07430"
     CDS             4629..6281
                     /locus_tag="VEV43_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYP23387.1"
                     /translation="MGATDGLKTLSQFFQPRNLQQLKKANALGPKNLLGLGYAFPWLL
                     LRGPSLGITSQMHSMSLGDKPALYDRDGMLTWRELDRRSNQAAHLLEARGIGPNDRVA
                     MLLRNGREWAEVALATQKLGVAAVPLNTWAKPKELKATLGGADPKLLVYDTAHAEQVK
                     ECARPGLPLMYVGSAADALEGSEPYHQELGRQPSSPPAPFTRDRGSPKVIIHTSGTTG
                     TPKGATRNASTTGISALAGLLAVVPYSRDDVIYCPAPLFHSFGLMTFTFAMVLGATLV
                     LPEKFDPEESLRLIDEHRATAASRVPVMMRRIVSLDDEVKAKYDMSALRIVLASGSVL
                     SEDLRRAATKVFGEVLYDLYGSTEIGWVAIATPEDMKTRPKTVGKPVEGNDVAIFSPE
                     GDKLPPGETGELYIKSDMLFEGYTSGDAKAERDGYTSIGDLGRLDDEGYLYVESRSDD
                     MVVVGGENVYPIEVEQVIEDVPGVNEVTVFGLDDDEYGQVLVAFVVGKVSEEDVRAAC
                     KRELASYKVPRRIEVVDELPRTSTGKVLKRELIEQLTNAAKE"
     gene            6290..7099
                     /locus_tag="VEV43_07435"
     CDS             6290..7099
                     /locus_tag="VEV43_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP23388.1"
                     /translation="MPSFGYEGFDIAFEQRGRKQGAAERPIVLIHGLLLPRKHNYLLA
                     DALADRGNRVILLDLLGHGESDQPTHSRHHSMELYARQVVALLDHLDVPEAVVAGTSL
                     GANVTLEVAAHAPDRTRGMFVEMPVLERAAPAAAAIFLPLTIAYAQAERPFSWFASAM
                     RRVPRGVSLYWDVVLDTLSRDPIPSAAVLHGLLTGRLAPHPSVREKIDAPALIVAHPH
                     DILHPFSDAAALARELPNSELVAARSFFELRFPPNRLSNRIADFLDEVWAG"
     gene            7096..>7305
                     /locus_tag="VEV43_07440"
     CDS             7096..>7305
                     /locus_tag="VEV43_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYP23389.1"
                     /translation="MTPPRREPDPCVVVIFGASGDLTARKLLPALFNLRREGLAPGAT
                     SVLGTARTEMSDAEFATRMRRAVTDH"
BASE COUNT         1142 a         2517 c         2572 g         1074 t
ORIGIN      
        1 gtcgagacag agttctcgct cgtccgcttc gggggcgacg ccgagaaggc gaagcagccc
       61 tacgccgggc tcgatcccct gacggccgag gacatagcgg actgcatcgc gtgggccgtc
      121 acgcggcccc cgcacgtcga catagacgag atagtcgtgc gcccggtggc acaagcccgg
      181 gcgaccgagg tagcgcgcaa gccctagcgg cgctggtggt cggcctcgtc ggcgtcagag
      241 tccgggctga cgcggtgctc tatgtccgtc tgctccttcg gcgggatctt gaagctcagg
      301 atcgccgtcg ccgtcagcac cccgatgatc accgtcagcg acagccagat cgggacgtgg
      361 atcgcgtggt cggtcaggcc gacgatctcg gtcccctcga gcagcatctt cacgcccacg
      421 aagccgagga tgatcgcgag gccgtacttc agcaggtgca acctgtccat gcccccggcg
      481 agcaggaagt acagcgcccg cagcccgagg atcgcgaaca cgttcgacgt cagcacgatg
      541 aacggctcct tcgtcaccgc gaagatcgcc gggatcgagt cgatggcgaa gaagatgtcg
      601 gtcgtctcga tgaacagcag gacgatgaac agcggcgtcg cgacccgctt gccgttctcc
      661 atcgtgaaca gcttctgccc gtcgaggttg tgcgtcgtgc ggaatcgctt gcgcgcgaac
      721 ttgagaaccg ggttgtcctc gggattgacg tgctcggcgg accccttcgc gatcttgtag
      781 gccgtgtaga gcaggaagag cccgaagagc gggatcaccc agtcgaagtt ctggacgagc
      841 gcagtcccca gggcgatgaa gatgcccctg aagatcatcg ctccgaggat cccgtagaag
      901 aggacctgat gctggtgcga caacgggatc ttgaagtagg cgaagatcac gacgaacacg
      961 aacatgttgt cgaccgacag cgagtactcg atcaggtagc ccgcgaagta ctcccccgcg
     1021 tccttcgagc cctggacgaa ccacagcccg acgccgaacg cgagcgcgag accgacccag
     1081 atcgcgaccc acgtgccgga ctccttcgtc gtcggcgtgt gggcctcggt gtggaacagc
     1141 ttgagatcga cgagcagcat cgcgatgacg aacgcgatga agccgatgta aagccagggc
     1201 gacacttcca acaggtgtcc tccggttctc gggcgggctg gactatggtc ggggccgcag
     1261 acctcgacag ggagcacggg tgcgcagccg gacagcaagg atagtggcgg tcgccgcgtt
     1321 cgccgcgacg ctgctcgcgg cttgctcggg tgacgattcg aagggggagt tcgtcgcggc
     1381 cgcggacgag gcgtgccgtg aggccgacga gcgcatctcc gagatcggcc ggccccgggt
     1441 cgaggagggg atccacgact acgtcgagca ggcgcaggag atctcggccg acctcgccgc
     1501 cgacctccgc gatctcgacc ctccgccgga ggacgtcgcc agggtggagg gcatgatcga
     1561 cgacctggag gcggcgacgg acctgctcga gcccctagcc caggcctcga tcgaccggga
     1621 cgccgaagcc ctcgaggcga tccagcagga agtccagaga atcaccgacg acgtgagcga
     1681 gttcgccgag tcgtacggct tcgaggtctg cggcgcgaag gtcctcgacc ccatgcgttg
     1741 agcgggatcg tccacgcctc cgccgcggcg gtggcgttcg ggacgctcgc cgtcttcgcg
     1801 aagctcgcgt acgaggacgg ggccgagctc ctccccctgc tcgcgacgcg cttcgtcgtc
     1861 gccggggcgc tgctcgtcct gtaccacgtg gtgacgcggc agcggctgtg gccgggccgg
     1921 cgggacgccg tgcgcttgct ggccctcggc gggctcgggt acgcggccga gtcggcgctg
     1981 ttcttcatgg gcgtcgagcg cgcccccgca gcggtcgtga gcctcgtctt cttcacgtat
     2041 ccgatgtgga ccgcgttgct tgcgttcgcg acgcggatgg agcgctacag caaccgcgtc
     2101 gcggtgtcgc tggtgctcgg gagcgccggg gtcgggctga tcttctccgt ccggctgggg
     2161 tcgttgctcg gccccctctt cgcgctgctc gccgcgatgt ctcttgccgt ctaccttctc
     2221 gtcgcccagg tcgtggcgcg cgacgtggcc ccgtcggtgg cggcgacgtg ggtctccctg
     2281 ggggccgcgg tcgcgttcgg tggcggaacg ctcgtatcgg gggactggct cgcgtcggac
     2341 gcgctggacg aggcggccgc gctcgggatc gcgacgtcga tcgcgttcgt gctgctgtac
     2401 cgggcgatag cgctgatcgg ctcggcgcgg acggccgtgg cgagcatgct cgagccggtc
     2461 gcgacgatcg tgctcgccgc gctgctgctg gacgaggcga tcacggcgcg ggtggcgctg
     2521 ggggccgtgc tggtggtggc tgcgctgccg atcctggcga cggcgccgac ggtcgagccg
     2581 gccccaccgg cggggccgtg accgaggcgc ggcgggccga gtcggggtgg gggggccggg
     2641 cgagtccgca gaagcccgag acctcgcctt cggccgggcg tggcatccgt tcatcgaact
     2701 gcacgaggcg gcgcgacgcc tggcgagcga ctagtcgggg ggccgcgacc gagcggtgca
     2761 gccggtgcag ccggtgcaac gtgcaccgca tgcaccgcat gcaccgcatg caccgcatgg
     2821 gacccgcatg gaccgcatgc accgctcgct ttcggggcaa cggcggtagc ctcgccgcca
     2881 tgtctcgggg aggccgcctt ctacgcaccg tcgtcgttct cgccgtcctc gtcgcgggag
     2941 ggttcggcgc ggctgcggcc ttgcggggtg acccggacca acccgagggg ccgggcgggc
     3001 tcccgccgcc cgacgagcgc acgttcgaga ccgacgaccc cgtcgagagg gcgtgcgcgc
     3061 tcgacaagcc gctgctcgcc cgggtgtggc gcggctacca cccgaagcac tccgaggacg
     3121 tgacgttcgt gccgctcgag ccgaactact ccggcgcctt cgacgtcacc agccattccg
     3181 ggccgtggga ctacctgcag aacgtcccgc tggtgttcta cggccccggc gtcatcgcgc
     3241 ccgagggccg ggtgcgcggc cccgccacgc tcgccgacgt cttcccgacg gtgggcgcca
     3301 tggccggcgt gccgctgccc gagcgcgagg gccgcgtgct ccacgacgcg ctcggcgaga
     3361 ccgagtcggc cccccggctc gtcgtcacga tcatgtggga cggcgtgggc cggaacgtgc
     3421 tggaacggtg gccgggccgc tggccgaacc tcgctcgcat ggagcgcgag ggcacgtcgt
     3481 atctgaccgc gaccgtcggg tcttcaccgt cgatcacgcc ggcgacgcac tcgacgctgg
     3541 gggccggtgt ctacccgcgc acgcacgggg tcacggcgat caacatccgc acgaagaacg
     3601 gcgacgtccg caccgcgttc aaccagcgcg accccggcga cctgaagcag acgacgttcg
     3661 cggacgagat cgacctcgcg ctcgggaacg agccgctcgt cggcatgctc gcctggaagt
     3721 cctggcacat cgggatgatg ggtcacggga cgcagacgcc gggcggcgac gcggaccagc
     3781 tcgcgatcat cggcggcgac gagcgcatca ccgggaaccc gctctacgac tcggcccccg
     3841 agtacctcga gaacttcggc gggctcgagg agcacgccga cgagctcgac ctcgaagacg
     3901 gcgaggccga cggcaagtgg cgcggccacg ggatcctgga gctccacgac aaccccgcgt
     3961 gggtcgagtg ggagaccgac gcgatcctcg ccatgctcga gaacgagggc tacggcgacg
     4021 acgaggtcac cgacttcttc ttcacgaact acaagccgac cgacatcgtc gggcaccagt
     4081 acacgatgga ctctccggag atgggcgacg tgctcgaggc gcaggacgcc gcgctcggcc
     4141 gtctgctcga ctggctcgac gccaacgtca gcgactacgc ggtgatcctc agctccgacc
     4201 acggccacac cccctcgccc gagcgcagcg gcgcgtggcc gctcctccag ggacagctcc
     4261 aggaggacat agacgcggcg ttcgacgtcc cgaaggggaa gtcgctcgtc gagcaggcga
     4321 accccgtcgg tccgttcctg gacaaggaag tgatgaacga gctcggcgtc acgggggccg
     4381 aggtcgccga gttcctgaac ggctaccgga tccgcgacaa ctggggcggg ggtgagctgc
     4441 ccgagggatt cgaagaccgg ggggacgaga acgtgatcgc cgcggcctgg tcgcgccggc
     4501 agtacggaga ggtgatgcgc tgcgcgttcg ggtcggagaa gccgccggct tcggcgggtc
     4561 tgagggccga gccggaggcc gcctcggcgg gacccgcgac cgatacggag taatacgctt
     4621 gctcccccat gggagctacc gacggcctga agacgctcag ccagttcttc cagccgcgta
     4681 acctccagca gctcaagaaa gccaacgccc tcggccccaa gaacctcctc ggcctcggct
     4741 acgcgttccc ttggctgctg ctgcgcgggc cctcgctggg tatcacctca cagatgcact
     4801 cgatgtcgct cggcgacaag ccggctctct acgaccggga cgggatgctg acgtggcgcg
     4861 agctcgaccg gcgctcgaac caggccgcgc acctgctgga ggcccgcggg atcggcccga
     4921 acgaccgcgt tgcaatgctt cttcgcaacg ggcgtgagtg ggccgaggtc gccttggcga
     4981 cgcagaagct gggcgtcgcc gcggtcccgc tcaacacgtg ggcgaagccg aaagagctga
     5041 aggcgacgct cggcggtgcg gacccgaagc tcctcgtcta cgacaccgcg cacgccgagc
     5101 aggtgaagga gtgcgcccgg cccggacttc cgctgatgta cgtcgggagc gcggccgacg
     5161 ccctcgaagg gtcggagccg taccaccagg agctcgggcg ccagccgtcg tcgcccccgg
     5221 caccgttcac gcgcgatcgc ggcagcccca aggtcatcat ccacacctcc ggaacgacgg
     5281 ggacgcctaa gggggccacg cgcaacgcgt cgacgaccgg catctcggca ctcgccgggc
     5341 tgctcgcggt cgtcccgtat agccgcgacg acgtcatcta ctgcccggcc cccctgttcc
     5401 actcgttcgg cctgatgacg ttcacgttcg cgatggtcct cggcgcgacg ctggtcctcc
     5461 cggagaagtt cgatccggag gagtcgctcc gcctgatcga cgagcaccgc gcgacggccg
     5521 cgtctcgagt gccggtgatg atgcgccgga tcgtctcgct cgacgacgag gtcaaggcga
     5581 agtacgacat gtcggcgctg cggatcgtgc tcgcgagcgg gtcggtgctg tccgaggacc
     5641 tgcggcgcgc cgccacgaag gtgttcggag aggtgctcta cgacctctac gggtccaccg
     5701 agatcgggtg ggtcgcgatc gcgacgccgg aggacatgaa gacgcggccg aagacggtgg
     5761 gcaagccggt cgagggcaac gacgtcgcga tcttctcccc cgagggtgac aagctgccgc
     5821 ccggcgagac cggcgagctg tacatcaaga gcgacatgct gttcgagggc tacacgtccg
     5881 gcgacgcgaa ggccgagcgc gacggataca cgtcgatcgg agacctcggc cgcctcgacg
     5941 acgagggcta cctctacgtc gagtcgcgct cggacgacat ggtcgtcgtc ggcggcgaga
     6001 acgtctaccc gatcgaggta gagcaggtga tcgaggacgt tcccggtgtg aacgaggtga
     6061 cggtgttcgg cctcgacgac gacgagtacg ggcaggtcct agtcgccttc gtcgtcggga
     6121 aggtctcgga ggaagacgtg cgcgcggcct gcaagagaga gctcgcgtcc tacaaggtcc
     6181 cgcgccgcat cgaggtcgtg gacgagctgc cgcgcacctc gaccggcaag gtcctcaagc
     6241 gcgagctcat cgagcagctc accaacgcgg cgaaggagta agccgcccga tgccctcctt
     6301 cggctacgaa ggcttcgaca tcgccttcga gcagaggggg cgcaagcagg gggccgccga
     6361 gcgtccgatc gtcctgatcc acggcctgct cctgccccgc aagcacaact atctcctggc
     6421 cgacgcgctg gccgaccgcg gcaaccgcgt gatcctgctc gacctcctcg gccacggcga
     6481 gagcgaccag cccacgcact cgcgccatca ctcgatggag ctgtacgcgc gccaggtcgt
     6541 cgcgttgctc gaccacctcg acgtgcccga ggcggtcgtg gccgggacgt cgctgggcgc
     6601 gaacgtgacg ctcgaggtcg ccgcacacgc ccccgaccgg acgcgcggga tgttcgtcga
     6661 gatgcccgtg ctcgagcgcg cggcccccgc agccgccgcg atcttcctcc cgctgacgat
     6721 cgcgtacgcg caggcggaac gaccgttcag ctggttcgcg tcggcgatgc gacgggtccc
     6781 gcgcggcgtg tcgctgtact gggacgtcgt ccttgacacc ctgtcgcgcg acccgatccc
     6841 ctcggcagcc gtgctccacg gcctgctgac gggacgcctc gctccgcacc cgagtgtgcg
     6901 cgagaagatc gacgcccccg cgctgatcgt cgcccacccg cacgacatcc tgcatccgtt
     6961 ctccgacgcc gccgcgctcg cgcgtgagct cccgaacagc gagctcgtcg cggcccggtc
     7021 gttcttcgag ctccgcttcc ctccgaaccg cctctcgaac cggatagccg acttcctcga
     7081 cgaggtctgg gccgggtgac gcctccgcgg agagagcccg acccctgcgt cgtcgtgatc
     7141 ttcggcgcct cgggcgacct cacggcgcgg aagctgctgc cggcgttgtt caacctgcgg
     7201 cgcgagggcc ttgcgcccgg ggcgacgtcc gtcctgggga ccgcccggac ggagatgtcc
     7261 gacgcggagt tcgccacgag gatgcgccgc gcggtgaccg accac
//
LOCUS       DASQDO010000263         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_639190, whole genome shotgun sequence.
ACCESSION   DASQDO010000263 DASQDO010000000
VERSION     DASQDO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639190"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1501
                     /gene="trpE"
                     /locus_tag="VEV43_07445"
     CDS             26..1501
                     /gene="trpE"
                     /locus_tag="VEV43_07445"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HYP23390.1"
                     /translation="MAHRPGRVEFARLLEDNVIVPVWREVLADALTPVGAFLRLDPRP
                     HGFLLESVEGGERWARYSFIGGDAFGVVTAKDGAVSLEGDAPVSPEEGEAPLAYVGRL
                     LDACRAPALEGLPPLHGGAVGFIGYDCVRELERLPDIPDDDLGLPDLALVLTRTFVVF
                     DHLLQKAFAITNVMRSEASPDELYDDAVARCDAVVDAFSAPLASPASELDFGAEPGAA
                     STVDDGEYERWVEVSREHIYAGDVFQVVPSRRFEAPLKGSSFNAYRALRALNPSPYMY
                     FLRFGEDFEIAGSSPEPLVRVTGDRVETRPIAGTRGRGATRAEDEELECDLLSDEKER
                     AEHVMLVDLARNDVGRVARYGTVRVEELMVVERYSHVMHIVSSVTGVLREDRTPFDAL
                     LACFPAGTVSGAPKVRAMEVIDSLEKTKRGPYAGVVGYFDFSGNLDTCITIRTIVAAG
                     GRAYVQAGAGIVADSDPAAEAEETRRKAEALLRAVAAAERF"
     gene            complement(1498..1755)
                     /locus_tag="VEV43_07450"
     CDS             complement(1498..1755)
                     /locus_tag="VEV43_07450"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HYP23391.1"
                     /translation="MSSDTPIAWLALEDGTPILASDGEEVGRVSTVVADEQKDIFSGV
                     TFKPGLLDGQRFVPASAIDHLTESAVHLNITAAQAAELDAY"
     gene            1797..2567
                     /locus_tag="VEV43_07455"
     CDS             1797..2567
                     /locus_tag="VEV43_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYP23392.1"
                     /translation="MTRPFFLLLLALGAACSTLPEGQKATKPATGPPEIRQATIDEHA
                     RQFDEEEPDRPAGSQDELAASVYIVAHLTDAGYVSRLDAVPVEDLVRSTNVVALPPSG
                     EEPAALVVVAYDTGEGSGTGGHSIATWLEVARALRAIEPEHDVQFVALGAEHATVGGG
                     QLGSRRLAQQLEDQGKSPPVFVVQDVEPGDGPLGAGGAAGAGLADEAKELGLPAATGT
                     TLAPGPGDVFEAAGFETTVVGGGAAAAGPALLAYLATL"
BASE COUNT          371 a          970 c         1011 g          391 t
ORIGIN      
        1 tgcttctacc ggagcttctg acgcgatggc gcacaggccg ggccgggtgg agttcgcgcg
       61 gctgctcgag gacaacgtca tcgtccccgt ctggcgggag gtgctggccg acgcgctgac
      121 gccggtcggc gccttcctac ggctcgaccc gcgcccccac ggcttcctgc tggagagcgt
      181 cgagggcggt gagcgctggg cccgctactc gttcatcggc ggtgacgcgt tcggcgtcgt
      241 gacggcgaag gacggagccg tctcgctgga aggcgacgcg cccgtttcac cggaagaggg
      301 agaggcgccg ctcgcgtacg tcggccgcct gctcgacgcc tgccgcgccc cggccctcga
      361 agggctcccg ccactgcacg gcggcgccgt cggcttcatc ggatacgact gcgtgcgcga
      421 gctcgagcgc ctgccggaca tccccgacga cgacctcggc ctgccggacc tggcgctcgt
      481 gctcacgcgc acgttcgtcg tcttcgacca cctcctgcag aaggcgttcg cgatcacgaa
      541 cgtcatgcgt tcggaggcgt cgccggacga gctctacgac gacgcggtcg ctcgctgcga
      601 cgcggtcgtc gacgcgttct cggcccccct ggcctcgccc gcctcggagc tcgacttcgg
      661 ggccgagccc ggagccgcct ccaccgtcga cgacggcgag tacgaacggt gggtcgaggt
      721 ctcgcgggag cacatctacg cgggcgacgt cttccaggtc gtgccctcgc gccgcttcga
      781 agcgcccctc aagggatcgt cgttcaacgc gtaccgcgcg ctccgcgctc tcaacccgag
      841 cccgtacatg tacttcctgc gcttcggcga ggacttcgag atagccggct cgtcgccgga
      901 gccgctggtg cgcgtcaccg gtgaccgcgt cgagacccgg ccgatcgcag gcacgcgcgg
      961 caggggagcg acccgcgccg aggacgagga gctcgaatgc gatcttctct cggacgagaa
     1021 ggagcgcgcc gagcacgtca tgctcgtcga cctcgcgcgc aacgacgtcg gacgggtcgc
     1081 ccgttacggc accgtccggg tcgaggagct gatggtcgtc gagcggtact cgcacgtcat
     1141 gcacatcgtg tcgagcgtca ccggggtcct gcgcgaggac cgcactccgt tcgacgcgct
     1201 gctcgcgtgc ttccccgccg gcacggtctc cggggcgccg aaggtccgcg ccatggaggt
     1261 catcgactcg ctggagaaga ccaaacgcgg cccctacgca ggcgtcgtcg gctacttcga
     1321 cttctccggc aacctcgaca cctgcatcac gatccgcacg atcgtggccg ccggagggcg
     1381 tgcctacgtg caggcgggag cgggcatcgt cgccgactcg gacccggctg cggaggcgga
     1441 ggagacgcgg cgcaaggccg aggcgctgct gcgagcggta gcggcggccg agcgcttcta
     1501 gtaggcgtcg agctccgcgg cctgcgccgc ggtgatgttc aggtgcacgg ccgactcggt
     1561 gaggtggtcg atcgcactgg ccgggacgaa acgctggccg tccaggaggc ccggcttgaa
     1621 cgtgaccccg gagaagatgt ccttctgctc gtcggcgacg acggtggaga cgcggccgac
     1681 ctcctcgccg tcgctcgcga ggatcggcgt gccgtcctcg agggcgagcc aggcgatcgg
     1741 ggtatccgat gacatgtggc gagtgtacgg gcgcgggctc gcctagattc cccttcgtga
     1801 cccggccctt cttcctgctg ttgctcgcgc tcggcgcggc gtgctcgacg ctgcccgagg
     1861 gacagaaggc gaccaagccg gcgacaggcc ccccagagat acgacaggcc accatcgacg
     1921 agcacgcgcg ccagttcgac gaagaggagc cggaccgtcc cgccgggtcg caggacgagc
     1981 tcgcggcgtc ggtctacatc gtcgcccacc tcaccgacgc gggctatgtg tcgcgcctcg
     2041 acgcagtgcc ggtcgaggac ctcgtccggt cgacgaacgt cgtcgcgctg ccgccgagcg
     2101 gagaggagcc cgccgctctc gtcgtcgtgg catacgacac cggcgagggc tccggcacgg
     2161 ggggccactc gatcgcgacg tggctcgagg tggcgcgggc gctgcgggca atcgagccgg
     2221 agcacgacgt ccagttcgtc gcgctggggg ccgagcacgc caccgtcggg ggcgggcagc
     2281 tcgggtcgcg gcggctcgcc cagcagctgg aggaccaggg gaagtcgccg ccggtcttcg
     2341 tcgtgcagga cgtggagccg ggcgacggcc cgctcggggc cgggggagcc gcgggggccg
     2401 ggctcgcgga cgaggcgaag gagctcgggc tcccggcggc cacgggaacg acgctggcgc
     2461 ccggccccgg cgacgtcttc gaggcggcgg ggttcgagac gacggtcgtc ggtggcgggg
     2521 ccgccgccgc ggggccggcg ctgctcgcct acttggccac cctttagggt cgaacgggtg
     2581 tccttcctgg aagacctcgt agccggcgcg cacgcgcgcg tcgcggcggg caagcagacg
     2641 ctgaccgacg acgtgctcga gcagcggctc gcgtcggcgg cccccgcccg ctcgctcgaa
     2701 gccgcgctcg cggcccccgg gatgtcgctg atcgcggaga tca
//
LOCUS       DASQDO010000264         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_150064, whole genome shotgun sequence.
ACCESSION   DASQDO010000264 DASQDO010000000
VERSION     DASQDO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150064"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VEV43_07460"
     CDS             complement(<1..320)
                     /locus_tag="VEV43_07460"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HYP23393.1"
                     /translation="MIAAVVAGVALGVYSRVVDYFAPDWAGNSLALWLLVACVVGLGS
                     RSWGAGAARGCLALVVASSSYYAWRLFVMDDIGIRFATRAFTFWTALAVTAGFVAGGF
                     AHRGR"
     gene            complement(343..1635)
                     /locus_tag="VEV43_07465"
     CDS             complement(343..1635)
                     /locus_tag="VEV43_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23394.1"
                     /translation="MESLTLDQLRQPDERSLVFGPFVGTVPNDRAAAILEHRQRTIGG
                     TDLVPNVPEPIRQNFDRLRLAYVYGVLCYEIFTVVEQAVFFLLEHALGERLLEHYDHT
                     LPLVNWKTRDEKPLRVHNFPQVHEALRGKGGSHRKGEWALESPHGELQHFTGSVTNLL
                     KWARAQGFLRGQRSRWSEGFLVDRRNHAAHPAWHEIHSPADAAEAIRDVAELVNQLWG
                     SATPGGRLFPAPIERTPIAVGWNTGGDEWAVTLCQNLRTYGKDDWLYVLVRGVFRDDD
                     LTSYSTDYELTTFPTEWLWGPGSHHEAIDWLDREQPHPDTVDYLDRKLLVRVVGDNVE
                     LPRRPEVAAAFDDAREEGIWHLVLADFPLVAYGHVRASAAAKGECVQEGPCPACQAHT
                     LARGELPDVLDFLEARFGKIEREPPRGVAVPGYRSPMA"
     gene            complement(1638..2825)
                     /locus_tag="VEV43_07470"
     CDS             complement(1638..2825)
                     /locus_tag="VEV43_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23395.1"
                     /translation="MDFAHSERSLRLQDELGSFMDEHVYPAERTYYDQVFSQEDPHVL
                     PPVMEELKDEARRRGLWNLFLDLSNLDYAPLAEIMGRSPIAPEATNCAAPDTGNMEIL
                     AEFGTPEQKERWLEPLLAGEIRSCFGMTEPGVASSDPTNLSATIERDGDEYVITGRKW
                     WTSGAADPRCKVCLFLGRTDPGAEKYMQHSLVLVPMDAPGVDIVRSLPVFGYDAGHGH
                     CEIAFEGVRVPRSSLLGEEGGGFLIGQARLGPGRIHHCMRAIGMAERALELMCKRAHS
                     RVAFGIPLAKQGVIRDWIAEARIRIEQARLLTLKAAWIMDTAGKDKARTEISAIKVAA
                     AETATWVIDRAIQVHGGAGVSDDFPLAAMYAHARTLHIVDGADEVHKMVLARNELHRW
                     KPS"
     gene            2877..>3367
                     /locus_tag="VEV43_07475"
     CDS             2877..>3367
                     /locus_tag="VEV43_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYP23396.1"
                     /translation="MRAHRCFVAAALVAGLLYPSAALAVSAPDKGKAWSPKVKSEALY
                     GSKPPSEDPDVQQHFVEGHDGTQLYVETWLPEELNGKVPPKKVPTILIMTPYVSQGVE
                     EYPGNATTPNFVEYFNARGYAVAQHHVRGTGESGGCLEQTSTNQFKDGAAVVEYLGKE
                     APWS"
BASE COUNT          556 a         1162 c         1093 g          556 t
ORIGIN      
        1 cgcccgcggt gcgcgaaacc cccggcgacg aaccccgccg tcacagcgag cgccgtccag
       61 aacgtgaacg cgcgcgtggc gaagcggatc ccgatgtcgt ccatgacgaa caggcgccac
      121 gcgtaatagg aggagcttgc gacgacgagc gcaaggcagc cgcgggccgc tccggccccc
      181 cacgagcggg acccgagccc gacgacgcag gcgacgagca gccacaaggc gagcgagttg
      241 cccgcccaat ccggtgcgaa gtagtcgacg accctgctgt agacgccgag cgcgaccccg
      301 gcgacgaccg cggcgatcat cgcctggatc ggggcacggc cgtcacgcca taggagatcg
      361 gtagccggga accgcgacgc ccctcggggg ttcgcgctcg atcttcccga accgggcttc
      421 gagaaagtcg aggacgtcgg gaagctcacc tctggcgagg gtatgcgcct ggcatgctgg
      481 acatgggcct tcttgaacgc actcgccctt cgctgctgcg ctggcgcgga cgtgtccgta
      541 cgcgacaagg gggaaatccg ctaggactag atgccagatg ccttcctcgc gggcgtcatc
      601 gaatgcggcg gctacctccg gccgccgtgg cagttcgacg ttgtctccaa cgacccgcac
      661 gagcagcttg cggtcgaggt agtcgactgt gtccgggtga ggctgttctc ggtcgagcca
      721 atcgatcgct tcgtggtgtg atcccggccc ccatagccac tccgtaggaa aggtcgtaag
      781 ttcgtaatcc gtggaatacg acgtgaggtc atcgtcacgg aatacgcctc ggacgaggac
      841 gtaaagccag tcgtctttgc catacgtccg gaggttttga cataacgtca ccgcccactc
      901 gtcgccgccc gtattccatc ccactgcgat cggcgttctc tcgatgggtg cagggaagag
      961 ccgtcctccc ggcgtcgccg atccccagag ctgattcacg agctcagcga cgtccctaat
     1021 ggcttcggca gcgtctgccg gcgaatggat ttcgtgccat gcggggtgcg cggcgtgatt
     1081 gcgccggtcg actaggaaac cttctgacca cctgcttcgc tgtccccgaa gaaatccctg
     1141 agcccgcgcc cacttgagca agttcgtgac ggagccagtg aagtgctgta gctcgccgtg
     1201 cggactttcg agagcccact cgcctttcct gtgcgatcca cccttgcctc gaagagcctc
     1261 atgcacctga gggaagttgt gcactcggag cggtttctca tcgcgcgtct tccagttcac
     1321 gagcggcaag gtgtggtcgt agtgctccaa caaccgctcg ccgagagcgt gctccaggag
     1381 gaagaagact gcctgctcga ccacagtgaa gatttcgtag cagaggaccc cgtagacgta
     1441 agcgagacgc agccggtcga agttctggcg gatcggttcg ggaacgttgg gaacgaggtc
     1501 tgttccgccg atcgtcctct gtcggtgctc caggatggcc gccgcgcggt cgttaggcac
     1561 ggtcccgacg aacggtccga acacaagaga tctctcgtcg ggctgcctca gttggtcgag
     1621 cgtcagcgac tccacggcta ggacggcttc cagcggtgta gctcgtttct cgccagcacc
     1681 atcttgtgca cctcgtcggc gccgtcgact atgtgcagcg tccgcgcgtg cgcgtacatc
     1741 gccgcgagcg ggaagtcgtc cgacacaccg gcccccccgt gcacctggat cgccctgtcg
     1801 atcacccacg tcgcggtctc ggccgcggcg accttgatcg ccgagatctc cgtgcgcgcc
     1861 ttgtccttgc cggccgtgtc catgatccac gcggccttca gcgtcagcag ccgggcctgc
     1921 tcgatccgga tgcgggcttc cgcgatccag tcgcggatca ccccctgctt cgccagcggg
     1981 atcccgaacg cgacgcggga gtgcgctcgc ttgcacatca gctcgagcgc ccgctcggcc
     2041 atcccgatcg cgcgcatgca gtggtggatg cggcccggcc ccagcctcgc ctggccgatc
     2101 aggaacccgc cgccctcctc gccgagcagg ctcgaccgcg ggacccgcac gccctcgaac
     2161 gcgatctcgc agtggccgtg cccggcgtcg tacccgaaga ccggcagcga gcggacgatg
     2221 tcgacgcccg gcgcgtccat cgggacgagc acgaggctgt gctgcatgta cttctcggcc
     2281 cccgggtcag tacgtccgag gaacaggcag accttgcagc gcgggtccgc ggcgcccgag
     2341 gtccaccact tgcgcccggt gatcacgtac tcgtcgccgt cccgttcgat cgtcgcggag
     2401 aggttggtgg ggtcggagct cgctaccccc ggctcggtca tcccgaagca cgacctgatc
     2461 tcgccggcca gcagcggctc gagccagcgc tccttctgct cgggcgtgcc gaactcggcg
     2521 aggatctcca tgttgccggt gtcgggggcg gcacagttcg tcgcctcggg ggcgatgggg
     2581 ctgcggccca tgatctcggc cagcggcgcg tagtcgaggt tcgagaggtc gaggaagagg
     2641 ttccacagcc cccggcgcct cgcctcgtcc ttcagctcct ccatcaccgg gggcaggacg
     2701 tgggggtcct cctgcgagaa gacctggtcg tagtaggtgc gctcggccgg gtagacgtgc
     2761 tcgtccatga acgacccgag ctcgtcctgc aaccggagcg accgctcgct gtgcgcgaaa
     2821 tccatcggca gaggctactt gcaggacccg ctcgcccagg gccgaaccaa tcttccatgc
     2881 gtgcacatcg ttgcttcgtc gcggccgcgc tcgtcgccgg cctcctgtac ccgtccgccg
     2941 ccctcgccgt ctcggccccc gacaagggca aggcctggtc gcccaaggtg aagtccgagg
     3001 cgctctacgg ctcgaagccg ccgagcgagg accccgacgt ccagcagcac ttcgtcgagg
     3061 gccacgacgg gacgcagctc tacgtcgaga cgtggctgcc ggaggagctc aacggcaagg
     3121 tcccgccgaa gaaggtcccg acgatcctga tcatgacgcc gtacgtcagc cagggcgtcg
     3181 aggagtaccc gggcaacgcg accacgccga acttcgtcga gtacttcaac gcgcgcgggt
     3241 acgccgtcgc gcagcaccac gtccggggca ccggcgagtc cggcggttgc ctcgagcaga
     3301 cctcgaccaa ccagttcaaa gacggcgcgg cagtcgtcga gtacctaggc aaggaggccc
     3361 cctggtc
//
LOCUS       DASQDO010000265         6149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_272361, whole genome shotgun sequence.
ACCESSION   DASQDO010000265 DASQDO010000000
VERSION     DASQDO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272361"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VEV43_07480"
     CDS             complement(<1..832)
                     /locus_tag="VEV43_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23397.1"
                     /translation="MATTYTGVDVARLRGWLSNLARPERLADAHMAALLRAHGRAVDG
                     PALDVGRAGARLLTDAIDGLTATGLPHRVLDTCFVEGVKSRAAAARLGLSERQLSRER
                     SRAIALLAAQLTPAHERVPCGAPPALPVPFLARPPLAEALAAALRMHRRVHVTGPPGC
                     GKTSLIAAHAAASPARVFWYRPGPQAAVALPALLFDLGEHLAPDDPSLASYVRGALSD
                     LDVGLATRVALAGLARRERLLVFDDARTIDAAAGSFRAEALARLPRLGLVEVGGPAGA
                     G"
     gene            complement(837..1973)
                     /locus_tag="VEV43_07485"
     CDS             complement(837..1973)
                     /locus_tag="VEV43_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HYP23398.1"
                     /translation="MSRAPNRPPAHSTTREVPVHRTATRGGTPLVVGVVEARDLRKTF
                     TIPRRDARTKSGYQRELSQSRVNRLVKDLRADRVDLPTSILVNLRDYDPERHLVERRG
                     RFFFRHGGASLYVVDGQHRVEALARLVDEDEDRWGGFEIPFVCMLGASEREEIRQFYV
                     VNSTAKSVRTDLALDLLKQRAESEPALMDALVETGEAWKVRGQQIVEDLAKTRLWRGR
                     TRFPGEPKGETTIGSAGLVGSLRPLLNTPYFGSLTTENQVRILDAYWEGLARILPECF
                     VRPTEYTLQKTTGVNVLHGLLVSVLEYVRSVGRSVVEPDVYGDVLGGVLPELEGDTTS
                     GEIVRGPDFWLGSGEGAAGSYSSNAGRRVLTAKLRGMLPPIEVE"
     gene            complement(2126..5506)
                     /locus_tag="VEV43_07490"
     CDS             complement(2126..5506)
                     /locus_tag="VEV43_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23399.1"
                     /translation="MQPSPRAPRSSIAFVIALLIACVGAVVAPTASGQPSPAPSPSGS
                     ASPGPAGDALSVRLLNPSTGYDDPPKVSDRFDGVDTHYTIVAQTTGAAQSAILEASIA
                     PQNSDGGFDNEILIGELERVSSTSDVWQYDWDIPASLSDGTALVTVRAFVDTPAGFAE
                     TGSDAVEVSLGFEDTSPQPIGAWETVDVSWPEQEGVLGWYKPRVGAWRTLVDGTTSPG
                     ANYVQVLLSTTPAGEPPVFVACGSGPVTVQAGVPRFSARCTLDASTLPSAVTTIAVVA
                     EFRETNTSTRLPQAADVRVVDSYTLSPEDMSVRLTPVARRVLAPATACQAFTVHVRDE
                     HGRLVLGANVDVHASGPADELILAGTQMYVPDMAHATEQTLTCPGSETEVPMNPLPPA
                     TRNQGEHNVPGGLDVKHMENRNGTGLDGTSQAAGVAAFLLASENPGKTHVTVWVDDEE
                     IARENDQRPLDNDRLDPGEPTGTALMQWLTANPTLSLDPLGGTVPTDACFPFVVKARA
                     GTAALPELNVDVHATGPDAELDFCDPAGASKRRSPAPGTGANTHTAEDERESHHLSST
                     APPTQHTEGETDEAGNLVVGLTSPSPGDTTVVAGIDGEPGADDDVQGGAEASASGTVS
                     WATSAEGAELGFVNPSPYGGGTEGAGTGLQLPDDGGVAEVLVRVDLADSAPTVEIMLS
                     RDNRRTFSTIGEARRVAKTDLFRFEWPISLPDGTYVLRARIPGTEIVEDVTVTVGAGE
                     RLPMIPNPAFETLEIRKPEAGEGAPFTRRSTTVTGRASAGAEGVDVYYTKVPAKDTPR
                     LQDWIFCGYADLAGTGTARQDFSTPCTLTGSDQAGQVTGIAAITYDCTVDGCDANPFP
                     DPPADGAPAMREQGQKDTGQALRVFGYEAHPRLALEPAEVQATTGDCRPLEVVLRDQT
                     GQPIAGANADLHLDGAPAAYFCRPADAPSGFRAPDAGGHTPGELEAGHADGSPGVHAE
                     GETLPDGKLVFGVASAEPGDVQVTAWLDRNDDDLRDGDDLSDTSVVHWVAPAGRCSIT
                     GSEGPDVLRGTAGADVFCGLEGNDVIRARGGDDTVFAGEGADRVFGAGGRDSIRGGRG
                     RDFLNGGPGRDTCRGGLGRDRIVRCESTSPGPVRRSGV"
     gene            complement(5566..6081)
                     /locus_tag="VEV43_07495"
     CDS             complement(5566..6081)
                     /locus_tag="VEV43_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23400.1"
                     /translation="MKRRWSIPLAGALSVLVLASAAAQGCTPVEPKKWEGTITYGGGG
                     WLAANVFEHAGCNWNGADALNGTDGIVWDVSGYGGVTATLTQSSAGGTHHELQGFFLN
                     ENCERGGAWGFTEPGVPFTIGIPEGAKWIVVYQTYGGAQTNVTLETPGRKCEPVDAPK
                     PPKKKKPKNRN"
BASE COUNT          801 a         2204 c         2247 g          897 t
ORIGIN      
        1 gccccgcccc ggcggggccg ccgacctcga ccaggccgag ccgcggcagg cgcgcgaggg
       61 cctcggcgcg gaacgagccg gccgcggcgt cgatcgtccg ggcatcgtcg aacaccagca
      121 accgctcgcg ccgcgcgaga cccgcgaggg caacccgcgt cgcaagcccg acgtcgaggt
      181 cggagagcgc gccgcggacg tacgacgcga gcgacgggtc gtcgggcgcc aggtgctcgc
      241 cgaggtcgaa cagcagcgcc gggagagcga cggccgcctg ggggccgggc cggtaccaga
      301 acacgcgtgc cggggacgca gcggcatgcg ccgcgatcag agaggtcttg ccgcacccgg
      361 gcggccccgt cacgtgcacg cgccggtgca tccggagcgc cgcggcgagc gcctccgcga
      421 gtgggggccg cgcgaggaac ggcaccggga gcgcgggagg cgcaccgcac gggacgcgct
      481 cgtgggccgg cgtgagctgc gccgccagca gcgcgatcgc gcgcgaccgc tcgcggctca
      541 gctggcgctc ggacagaccg agccgtgccg cggcggcgcg gctcttcact ccctcgacga
      601 agcacgtgtc gagcacgcgg tggggcagcc ccgtcgccgt gagaccgtcg atcgcgtccg
      661 tcaggagacg cgcgcctgca cgtccgacgt cgagcgccgg gccgtcgacg gcgcgcccgt
      721 gcgcgcgcaa cagcgccgcc atgtgcgcgt ccgcgaggcg ttcggggcgc gcgaggttcg
      781 acagccaccc gcgcaaccga gcgacgtcga cgcccgtgta cgtcgtcgcc atgcgactac
      841 tcgacctcga tcgggggcag catcccccgc agcttcgccg tgagcacgcg ccggccggcg
      901 ttgctcgagt acgaccccgc cgcgccttcg ccggagccca gccagaagtc cggcccccgc
      961 acgatctcgc ccgacgtcgt gtcgccctcg agctcgggca ggaccccgcc gagcacgtcg
     1021 ccgtagacgt ccggctcgac caccgagcgc ccgaccgacc gcacgtactc gaggaccgac
     1081 acgagcaggc cgtgcagcac gttcacaccg gtggtcttct gcaacgtgta ctcggtcggg
     1141 cgcacgaagc actcgggcaa gatgcgcgcg aggccctccc agtacgcgtc gaggatccgc
     1201 acctggttct cggtcgtgag cgacccgaag tacggcgtgt tgagtagcgg gcgcagcgac
     1261 ccgacgagcc ccgcggaccc gatcgtggtc tcgcccttcg gctcgccggg gaaacgcgtg
     1321 cggccgcgcc acagccgggt cttggcgagg tcctcgacga tctgctgccc ccgcaccttc
     1381 catgcctcgc cggtctcgac gagcgcgtcc atcagggcgg gctcggactc ggcgcgctgc
     1441 ttgaggaggt cgagcgcgag gtcggtgcgg acgctcttgg ccgtcgagtt cacgacgtag
     1501 aactggcgga tctcttcccg ttcgctcgcg ccgagcatgc atacgaacgg aatctcgaac
     1561 ccgccccagc ggtcctcgtc ctcgtcgacc agccgcgcca gtgcctcgac gcggtgctgc
     1621 ccgtcgacca cgtagaggct ggcgccgccg tgacggaaga agaaccggcc acggcgctcg
     1681 acgagatggc gctcggggtc gtagtcgcgc aggttgacga ggatcgacgt cggcaggtcc
     1741 acgcggtcgg cgcggaggtc cttcacgagg cggttcacgc gcgactgcga gagctcgcgc
     1801 tggtagccgc tcttcgtgcg cgcgtcgcgc cgggggatcg tgaacgtctt gcggaggtcc
     1861 cgggcctcta cgacgccgac gacgagcggg gtaccgcctc gcgtcgcggt tcgatggacg
     1921 gggacctcac gggtggtgct gtgggcgggc ggtcgattcg gggcgcgcga cacgagggct
     1981 ccttgctccc tccccagtat gggtcgcgcc ctcgcgggtg cccggcctag ccggggcggc
     2041 acggccgccg acggcggcgt tgaaaaagtt agcgggggat gaggtccggg tcaaggatcc
     2101 gcgcaaggga cccgctttgc tcggctcaga cgccgctgcg gcggacgggg ccggggctcg
     2161 tcgattcgca gcgcacgatg cggtcgcgcc cgagcccgcc ccggcaggtg tcgcgccccg
     2221 ggcccccgtt gaggaagtcg cgcccgcggc ccccgcggat cgagtcgcgg ccgccggctc
     2281 cgaagacgcg gtctgcgccc tctcccgcga acaccgtgtc gtcgccccct cgcgcccgga
     2341 tcacgtcgtt gccctcgagc ccgcagaaga cgtcggcgcc tgccgtgccg cgcagcacgt
     2401 cgggaccctc gctgccggtg atcgagcagc ggcccgcggg cgccacccag tggaccacgg
     2461 acgtgtccga gaggtcgtcg ccgtcgcgca ggtcgtcgtc gttgcggtcg agccatgccg
     2521 tgacctggac gtcgcccggc tcggccgacg cgacgccgaa cacgagcttc ccgtccggca
     2581 gcgtctcgcc ctcggcgtgg acgcccgggg agccgtcggc atgtccggcc tcgagctcac
     2641 cgggcgtgtg gcccccggcg tcgggagcgc ggaaccccga cggggcatcg gcgggacggc
     2701 agaagtacgc cgccggcgcg ccgtcgagat ggaggtcggc gttcgcgccg gcgatcggct
     2761 ggcccgtctg gtcgcgcaag acgacctcga gcggccggca gtctcccgtg gtggcctgga
     2821 cctcggccgg ctcgagcgcg agccgcgggt gtgcctcgta gccgaacacg cgcagagctt
     2881 gccccgtgtc cttctggccc tgctcgcgca tcgccggagc gccgtcggca ggggggtcgg
     2941 ggaacgggtt cgcgtcgcag ccgtcgaccg tgcagtcgta ggtgatcgcg gcgatgcccg
     3001 tcacctgccc cgcctggtcg gagcccgtca gcgtgcacgg ggtgctgaag tcctggcgcg
     3061 ccgtcccggt ccccgcgagg tcggcgtagc cgcagaagat ccagtcctgc agacgcggag
     3121 tgtccttcgc cgggaccttc gtgtagtaca cgtcgacgcc ctcggccccc gcgctggcac
     3181 ggcctgtgac ggtggtgctg cgccgggtga acggcgcgcc ctcaccggcc tcgggcttgc
     3241 ggatctcgag cgtctcgaac gcggggttcg ggatcatcgg gaggcgctcg cccgcaccga
     3301 ccgtcaccgt cacgtcctcg actatctccg tgcccgggat ccgcgcgcgc agcacgtacg
     3361 tcccgtcggg gagcgagatc ggccactcga accggaacag gtccgtcttg gcgacgcgcc
     3421 gggcctcgcc gatcgtcgag aacgtccggc ggttgtcgcg cgagagcatg atctcgactg
     3481 tgggagcgga gtcggcgagg tcgaccctca cgaggacctc ggcgactcct ccgtcgtcgg
     3541 gcagctgcag gcccgtcccg gcaccttcgg tccctccccc gtaaggcgag gggttcacga
     3601 agccgagctc ggcaccctcg gccgacgtcg cccagctcac ggttcccgag gccgacgcct
     3661 cggcgccgcc ctggacgtcg tcgtcggctc cgggctcacc gtcgatccct gcgaccacgg
     3721 tcgtgtcgcc cggggaagga gacgtgaggc cgacgacgag gttccccgcc tcgtcggtct
     3781 cgccctcggt gtgctgtgtc ggtggtgcgg tcgagctgag gtggtgcgac tcgcgctcgt
     3841 cctcggccgt gtgggtgttc gcgccggtgc cgggggccgg agagcgtcgc ttcgaggcac
     3901 cggccgggtc gcagaagtcc agctcggcgt cgggtcccgt cgcgtgcacg tctacgttca
     3961 gctcgggcag ggcagccgtc cccgcgcgcg ccttgacgac gaacgggaag cacgcgtccg
     4021 tggggacggt gccgcccagc ggatcgaggg acagcgtcgg gttcgccgtc agccactgca
     4081 tcagcgcggt gcccgtcggc tcgccggggt cgagccggtc gttgtccagc ggccgctggt
     4141 cgttctcccg cgcgatctcc tcgtcgtcca cccacaccgt gacgtgggtc ttccccgggt
     4201 tctcggacgc cagcaggaac gccgccacgc cggccgcctg gctcgtcccg tcgaggccgg
     4261 tcccgttgcg gttctccatg tgcttcacgt cgagcccgcc ggggacgttg tgctcaccct
     4321 ggttccgggt tgcggggggc agagggttca tcgggacttc cgtctcggac ccgggacagg
     4381 taagtgtctg ctcggtcgcg tgcgccatgt cggggacgta catctgcgtc ccggcgagga
     4441 tgagctcgtc ggcgggcccc gaggcgtgga cgtcgacgtt cgcgccgagc acgaggcggc
     4501 cgtgttcgtc gcgcacgtgc accgtgaacg cctgacacgc ggtcgccggc gcgaggaccc
     4561 gccgcgcgac gggcgtcagc cgcaccgaca tgtcctcggg gctcaacgtg tacgagtcga
     4621 cgacgcggac gtccgcggct tgggggaggc gcgtgctggt gttggtctcg cggaactccg
     4681 cgacgacagc gatcgtcgtg accgcggacg gaagcgtcga cgcgtccaac gtgcaccgcg
     4741 cggagaagcg ggggactccg gcctgaaccg tgacgggtcc cgagccgcac gcgacgaaca
     4801 cgggcggttc gcccgccggc gtcgtcgaca gcaagacctg gacgtagttc gcgcccgggc
     4861 tggtggtccc gtcgacgagc gtgcgccagg cgccgacgcg cggcttgtac cagccgagga
     4921 cgccctcctg ctcgggccag gagacgtcca cggtctccca tgcgccgatc ggttgggggc
     4981 tcgtgtcctc gaacccgagg ctgacctcga ccgcgtcgct gccggtctcg gcgaaccccg
     5041 cgggcgtgtc gacgaaggcc ctgacggtga cgagggccgt cccgtcggac agcgacgccg
     5101 ggatgtccca gtcgtactgc cacacgtcgg acgtggacga gacccgctcg agctcgccga
     5161 tcagtatctc gttgtcgaac ccgccgtcgc tgttctgagg cgctatcgac gcctcgagga
     5221 tcgccgactg ggccgccccg gtcgtctgcg cgacgatcgt gtagtgggtg tcgaccccgt
     5281 cgaaccggtc gctgaccttg ggagggtcgt cgtagcccgt gctcgggttg agcagcctca
     5341 ccgagagcgc gtcacccgcc gggccgggcg aggccgagcc ggacggcgag ggggccggag
     5401 agggttgccc cgaggccgtc ggcgcgacga ccgcgccgac gcacgcaatc agaagagcga
     5461 tcacgaaggc gatcgacgag cgcggcgcgc ggggggaagg ctgcacggat gacatcctga
     5521 gtgggttcgc taagccgtcg aggtacttgt caagacattt cgtgtctagt tacggttctt
     5581 cggcttcttc ttcttcggcg gcttcggggc gtcgacgggc tcgcacttgc ggcccggcgt
     5641 ctcgagcgtc acgttcgtct gggcgccgcc gtaggtctgg tagacgacga tccacttggc
     5701 cccctcgggg atcccgatcg tgaacggcac gcccggctcg gtgaagcccc aggccccgcc
     5761 gcgctcgcag ttctcgttga ggaagaaccc ctggagctcg tggtgcgtcc cgccggcgga
     5821 cgactgcgtc agcgtcgccg tgacgccgcc gtaacccgac acgtcccaga cgatcccgtc
     5881 ggtgccgttg agggcgtcgg cgccgttcca gttgcagccc gcgtgctcga acacgttcgc
     5941 cgcgagccac ccgccaccgc cgtacgtgat cgtcccctcc cacttcttgg gctcgaccgg
     6001 ggtgcagccc tgtgctgcgg cggacgccag taccaacacc gagagtgctc ctgcgagggg
     6061 tatggaccag cggcgcttca tggcgagtct ccttctattt ctacttgctg gatgttgggt
     6121 tcgcccggga gaagggtcat tccttcacc
//
LOCUS       DASQDO010000266         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1006336, whole genome shotgun sequence.
ACCESSION   DASQDO010000266 DASQDO010000000
VERSION     DASQDO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006336"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VEV43_07500"
     CDS             <1..222
                     /locus_tag="VEV43_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HYP23401.1"
                     /translation="VTEDLAAARRFTSEVDSGSIFVNASTRFSDGGEFGYGAEIGIST
                     QKLHARGPMGLEALTTTKYVVWGTGQVRT"
     gene            350..970
                     /gene="nadD"
                     /locus_tag="VEV43_07505"
     CDS             350..970
                     /gene="nadD"
                     /locus_tag="VEV43_07505"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HYP23402.1"
                     /translation="MTRRMDVRRLAVFGGTFDPIHNGHLAMATALLDSFSLDRVLFVP
                     AGRPWQKEGYSGAEDRWMMTVLAAATNPRFEASRVEIDRKGPTYTVDTLAILRDFYGP
                     EAELYFVAGIDAVLTLGTWHRADGLAALAEIIAVPRWGFDAGALRPGEGWPRIHFAEV
                     PEVDVSSTEVRRRVREGLPIDELVPPDVASYVREQDLYRQPSEKSA"
     gene            967..2757
                     /locus_tag="VEV43_07510"
     CDS             967..2757
                     /locus_tag="VEV43_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HYP23403.1"
                     /translation="MSLGNWWDERKRRQAERDAAEYGDWEDPTWADPDDPAPDAAPEP
                     VAEGPEAEPEPVVEPEPVVEEPEAEPEPVVDEPAVEEGRPLIPREAWWKRRLLGTPER
                     HQHEPVAEAAVAEEPEADLEPAAEVEPEPEILPDEIEAEPIAEEEEAFEEVHYDLGPL
                     DDEEMLAEPDPEIDLTDSGEEGLPPLGHDFPGPRRARLETQRRRKRRQELKGGMIAAA
                     VAAVVAVAGAVAILSRDEAPLPPKGAAAAGPERQDTITTTLLFGTKERRPEAGPTWMT
                     LLSYDSATGRGAVVYIPAHTATGVPGRGLLPVGEALSSGGVPLLLLATETLLGTPVDQ
                     SVELSDADAAVLLRETGPLTVDVPQDVRISAGPGQARLIFGAGPQRLSPDFLVDLLYV
                     KGLEGDDVELGSRHLAFWSSLFEAFAFDADGLAEAVRGSGAALGEADVPASEVAKLFE
                     LLAEAETSDRVLASLPVRQVGVGGSELYQVDDEEVEDFLRDTIGEQAVTGKEVRIQIL
                     NGNGAPGVGQYVAERLGGEGFRVILSGNARRLNYRETLVITYDRSSKGVGLAERTRDL
                     IGVGEVQVAAQSQGIVDLTIVVGKDFLETL"
     gene            2754..3176
                     /gene="rsfS"
                     /locus_tag="VEV43_07515"
     CDS             2754..3176
                     /gene="rsfS"
                     /locus_tag="VEV43_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HYP23404.1"
                     /translation="MTINDEGLVLEAAEAASAKKAERIVILDVSEQLVITDYFLICSG
                     NTQRQVRTIADEVQRRLSESHSMKPFRREGEREARWVLLDYVDFVVHVFHTDDREYYD
                     LERLWADARVVPFEEDWARASSDATVRGDDEPAAAEAT"
     gene            3148..3777
                     /locus_tag="VEV43_07520"
     CDS             3148..3777
                     /locus_tag="VEV43_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23405.1"
                     /translation="MSQPRRKRRRRRKPSGEGAQKPRQEGQQVREQASDLDASGRNGS
                     GRSRNRRRRRRGSGPGIASPKSSEDLVRALPKERPAQLTAPPDGQSLDKIIGELQSNY
                     GVPAYPQEYRITIKVAEEKPAAATQPAPPKTAPAPDAAPKREKAPAPPRVGPEGADEE
                     PAGERPAKRRRSRRRRGRGRSGGPSGGGGQGGSSSSPDAPPPPPPPPPP"
     gene            3821..3893
                     /locus_tag="VEV43_07525"
     tRNA            3821..3893
                     /locus_tag="VEV43_07525"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3854..3856,aa:Ala,seq:ggc)
     gene            4075..4311
                     /locus_tag="VEV43_07530"
     CDS             4075..4311
                     /locus_tag="VEV43_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYP23406.1"
                     /translation="MKRTTVKLPDEVDARLRHEAERRGMTVSELTREAIAAHLGQPER
                     RRLLAAKAGRSGTPDTAARVEEILAMELDRSRSS"
     gene            4296..4709
                     /locus_tag="VEV43_07535"
     CDS             4296..4709
                     /locus_tag="VEV43_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYP23407.1"
                     /translation="MALLVDSGPLYAYADADDRAHEPSLELLETHPGPLLVPVLCITE
                     VAYLIESRLGEAAQVRFLGDLAGGALIPEPVVPGDWLRIAELVWGYRDLPLGTVDASV
                     VAAAERLNVATVATLDHRHFGVVKPRHVEGFDLLP"
BASE COUNT          747 a         1655 c         1713 g          659 t
ORIGIN      
        1 gtgacggagg acctcgccgc cgcgcggcgc ttcacgtccg aggtcgactc cggttcgatc
       61 ttcgtcaacg cttcgacgag gttctccgac ggcggcgagt tcggctacgg cgccgagatc
      121 gggatctcga cgcagaagct ccacgcccgc ggcccgatgg ggctggaggc tctgacgacg
      181 acgaagtacg tcgtgtgggg gacgggccag gtccgcacgt aggtctctca cgggaccctc
      241 gggatttgtc agacaaatgt ctgacaattt ccgagggacc gttcgcggcc cccgcggccg
      301 ccatctcggg tcccgcccgc tttgacggca acttcccgcc ggtttcgggt tgactagacg
      361 catggacgtg cggcgactgg cggtcttcgg ggggacgttc gacccgatcc acaacggcca
      421 cctggcgatg gcgaccgcgt tgctcgactc tttctccctg gaccgggtgt tgttcgtccc
      481 ggcggggcgc ccgtggcaga aggagggcta ctcgggggcc gaggaccggt ggatgatgac
      541 cgtcctcgcc gcggcgacga acccacgctt cgaggcgtcg cgggtcgaga tcgaccgcaa
      601 aggccccacc tacacggtcg acacgctggc gatcctgcgc gacttctacg gtcccgaggc
      661 cgagctctac ttcgtagccg ggatcgacgc ggtgctgacg ctcgggacgt ggcaccgcgc
      721 cgacgggttg gcggcgctgg ccgagatcat cgccgtgccc cgatggggtt tcgacgccgg
      781 cgcgctgcgt ccgggcgagg ggtggccccg catccacttc gccgaggtcc cggaagtcga
      841 cgtctcgtcg acggaggtcc gccggcgggt gcgagaaggc ctcccgatcg acgagctggt
      901 cccgccggac gtggcgtcgt acgtccggga gcaggacctt taccggcagc cgtcggagaa
      961 gtcggcatga gcctcgggaa ctggtgggac gaacgcaagc gccgccaagc cgaacgggac
     1021 gcggccgaat acggcgactg ggaagacccg acctgggccg accccgacga cccggcaccg
     1081 gatgccgccc ccgaaccggt ggccgagggg ccggaagccg agcccgagcc ggtagtcgag
     1141 cccgagccgg tagtcgagga gccggaagcc gagcccgagc cggtagtcga cgaacccgcc
     1201 gtcgaggagg gccgcccgct gatcccgcgg gaggcctggt ggaagcggcg gctgctcggc
     1261 acgcccgagc gacaccaaca cgaacccgtc gccgaagctg ccgtcgcaga ggaacccgag
     1321 gccgacctcg aaccggccgc cgaggtcgag cccgagcccg agatcctccc cgacgagatc
     1381 gaagccgagc ccatcgccga ggaggaggag gccttcgagg aggtccacta cgacctcggc
     1441 cccctcgacg acgaggagat gctcgccgag cccgaccccg agatcgacct cacggactcg
     1501 ggcgaggagg ggctcccgcc gctcgggcac gacttccccg gcccccggcg ggcgcggctc
     1561 gagacccagc ggcgtcgcaa gcgccgccag gagctcaagg gcgggatgat cgccgccgcc
     1621 gtcgctgcgg tcgtcgcggt cgccggcgcc gtcgcgatcc tgtcccgtga cgaggcgccg
     1681 cttccgccga agggcgcggc ggcggccggg cccgagcggc aggacacgat cacgacgacg
     1741 ctgctgttcg ggacgaagga acgccgcccc gaggccggtc cgacgtggat gacgctgctc
     1801 tcgtatgact cggcgacggg ccggggggcc gtcgtctaca tccccgccca cacggctacc
     1861 ggggttccgg gccggggcct ccttcccgtg ggcgaggcgc tctcgtccgg cggggtgccg
     1921 ctgctgctcc ttgcgaccga gacgctcctc gggacccccg tcgaccagtc cgtcgagctc
     1981 tccgacgcgg acgccgccgt cctgctgcgc gagacgggcc cgctcaccgt cgacgttccc
     2041 caggacgtga ggatctcggc ggggccgggc caggcccggc tgatcttcgg ggccgggccc
     2101 cagcggctga gtcccgactt cctcgtcgac ctcctctacg tcaaagggct cgagggcgac
     2161 gacgtcgagc tcggctcgcg gcacctcgcg ttctggagct ctttgttcga ggcgttcgcg
     2221 ttcgacgccg acgggctcgc cgaggccgtc cggggctcgg gagcagcgct gggcgaggcc
     2281 gacgtcccgg cctccgaggt cgcgaagctg ttcgagctcc tcgccgaggc cgagacgtcc
     2341 gaccgggtgc tcgcctcgct tcccgtgcgc caggtcggcg tgggagggag cgagctctac
     2401 caggtcgacg atgaggaggt cgaggacttc ctgcgcgaca cgatcggtga gcaggcggtc
     2461 accggcaagg aggtgaggat ccagatcctc aacgggaacg gggcccccgg cgtcggccag
     2521 tacgtggcgg agaggctcgg cggcgagggc ttccgggtga tcctctcggg caacgcccgc
     2581 cgcttgaact accgggaaac gctggtcatc acgtacgacc ggtcgtcgaa gggagtcggg
     2641 ctcgcggagc gcacgaggga cctgatcggg gtaggagagg tgcaggtcgc agcgcagagc
     2701 caaggtatcg tggacttgac gatcgtcgtc ggaaaggact tcctcgaaac gctttgacta
     2761 tcaacgacga ggggctcgtc ctcgaggccg cagaggccgc ctcggccaag aaggccgaac
     2821 ggatcgtcat cctcgacgta tccgagcagt tggtgatcac cgactacttc ctgatctgca
     2881 gcgggaacac gcaacgccag gtgcggacga tcgcggacga ggtccagaga cgcctatccg
     2941 aatcccattc gatgaagccg ttccgccgcg agggtgagcg ggaggcgcgc tgggtgctgc
     3001 tcgactacgt cgacttcgtc gtgcacgtat tccacaccga cgaccgcgag tactacgacc
     3061 tcgagcggct gtgggcggac gcccgcgtcg tgccgttcga ggaggactgg gcccgggcat
     3121 cgagcgatgc aacggtaagg ggggacgatg agccagccgc ggcggaagcg acgtaggcgt
     3181 cgcaagccgt cgggcgaggg ggcccagaag ccgcgtcagg aaggccagca ggtcagggag
     3241 caggcgagcg acctcgacgc ctccgggcgt aacgggtccg gccgttcccg caaccgcagg
     3301 cgtcgccggc gggggagcgg gcccgggatc gcctcgccga agtcgtcgga agacctcgtc
     3361 cgagcactcc cgaaggaacg cccggcgcag ctgaccgcgc cgcccgacgg gcagtcgctc
     3421 gacaagatca tcggcgagct ccagtccaac tacggcgtcc ccgcgtatcc ccaggagtac
     3481 cggatcacga tcaaggtggc cgaggagaag ccggcggcag cgactcagcc ggcgccgccg
     3541 aagaccgcac ccgcgcccga cgcggccccc aagcgcgaga aggctccggc cccgcctcgg
     3601 gtcggccccg agggcgcgga cgaagagcct gccggcgagc gcccggcgaa gcggaggcgc
     3661 agcaggcgcc gccgcggccg gggccgttcc ggcgggccgt cgggcggagg gggccagggt
     3721 ggctcctcgt cgtccccgga cgcgcctcct ccacctccac caccaccccc gccttagctg
     3781 gtttagcatc gactgtccgg cggcccagag ggccgcgccc gggcccgtag ctcacttggt
     3841 agagcgccgc catggcatgg cggaggcaac cggttcgatc ccggtcgggt ccacccagaa
     3901 cagcgttcct tcagccgccc ttccggcagg ccgatgccct ccccgtgagg acggagcacc
     3961 gtgtcggcga cgcgcgctct gtggcggcgg gctacgtcga ggtcttcccg atccacatcc
     4021 ccacgagggg agaaaagaca ccagacagct tgcgataggt atgctggcat acctatgaag
     4081 cggaccacag taaagctccc ggacgaggtc gatgctcgtc ttcgccatga ggccgagcga
     4141 aggggcatga ccgtgtcgga acttacgaga gaggcgatcg cggcacacct cgggcaaccg
     4201 gagcgccgcc ggttgctcgc tgccaaggcc ggtcgcagcg gaacgcccga taccgccgcg
     4261 cgggtcgagg agatcctcgc catggagctg gaccgatcgc gctcctcgta gattccggcc
     4321 ccctgtacgc atacgcggac gcggacgaca gggctcacga accttcgctg gagctactcg
     4381 agacacaccc gggccctctc ctcgtccccg tgctctgcat caccgaggtc gcgtacctca
     4441 tcgaatcgcg gctcggggag gcggcgcagg ttcgattcct cggggatctc gcgggaggtg
     4501 ccctgatccc ggagcctgtc gtgccggggg actggctgcg catcgccgag ctcgtgtggg
     4561 gctatcgcga ccttccgttg ggaacggtgg acgcttccgt cgtcgccgcg gcggagcggc
     4621 tgaacgtcgc gaccgtcgcg acgctcgacc accgtcactt cggagtcgtc aagccgcgcc
     4681 acgtcgaggg cttcgacctc ttgccgtgac ggcggctacg gcatctacga tcctccgtat
     4741 gaaggctctg ctccgcgcgg tgtgccgtgc cctc
//
LOCUS       DASQDO010000267         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_700400, whole genome shotgun sequence.
ACCESSION   DASQDO010000267 DASQDO010000000
VERSION     DASQDO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700400"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VEV43_07540"
     CDS             <1..630
                     /locus_tag="VEV43_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYP23408.1"
                     /translation="ENPTGFEVDIADEIGKRLDLEVRWLEIPWTSIFAPGPKRFDMNI
                     NETTITAERDEVIDFSDPYFEANQAVLVPKGSEAEGATTIEALKGLRLAAQAQTTGLE
                     YIRSTIQPDEQPKVFPTTPAANQALLNQQIDAFVIDVPIAAGLVAENPDELVITGQFV
                     TNEEWGIVFEDGSGLVEHVNGALAEMKEDGTLAELQEKWLPGTDVPVIE"
     gene            733..1527
                     /locus_tag="VEV43_07545"
     CDS             733..1527
                     /locus_tag="VEV43_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HYP23409.1"
                     /translation="MRRNSALIASVSTFAVLGAIVFVFVTAPGAKKFQEAFLSPQEAL
                     DSVHPVWTGFVINLQLMVAGEALVLVFALLIAVVRGLPGPAAFPLRLLAIAYTDFFRG
                     VPLILVVFAIGLGIPALGIDILYTDNFFVYGLVSLVLVYSAYVAEVYRSGIESVHESQ
                     VAAARSLSLTHFQSLRHVVVPQAVRRVVPPLLNDFIGLQKDTALVAFVGVLDAFHQAQ
                     DRMAITYNFTALMVAALFFVALTIPLARVTDWLVERDRRKMRALGG"
     gene            1524..>2158
                     /locus_tag="VEV43_07550"
     CDS             1524..>2158
                     /locus_tag="VEV43_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HYP23410.1"
                     /translation="MSANGSALLLEDVCKRFGANEVLRGIDLDVGKHEVVCLIGASGS
                     GKSTLLKCVNLTEKVSSGRIVVEGHEITARGVDVNRIRRNIGIVFQAFNLFPHMTVLR
                     NVTLAPRKVLGKSRQEAEAEGLELLERFGLSDKRDEYPDRLSGGQQQRAAIVRALAMR
                     PKILLLDEVTSALDPELVAEVLSVIRELAESGMTMVIATHEMGFARDIADRV"
BASE COUNT          334 a          767 c          721 g          336 t
ORIGIN      
        1 gagaacccga ccgggttcga ggtggacatc gccgacgaga tcggcaagcg gctcgacctc
       61 gaggtgcgct ggctcgagat cccgtggacg agcatcttcg cgcccggccc gaagcgcttc
      121 gacatgaaca tcaacgagac gacgatcacg gccgagcggg acgaggtgat cgacttcagc
      181 gacccgtact tcgaggcgaa ccaggcggtc ctcgtgccca aggggagcga ggcggagggc
      241 gcgacgacga tcgaggcgct gaaggggctc cggctcgctg cgcaggccca gaccacgggc
      301 ctcgagtaca tccgctcgac catccagccc gacgagcagc cgaaggtctt cccgacgacc
      361 ccggccgcga accaggcgct cctgaaccag cagatcgacg cgttcgtcat cgacgtgccg
      421 atcgccgccg gcctcgtggc cgagaacccc gacgagctcg tgatcacagg gcagttcgtc
      481 acgaacgagg aatggggcat cgtcttcgag gacggaagcg gcctcgtcga gcacgtgaac
      541 ggcgccctcg cggagatgaa ggaggacggc acgctcgccg agctccagga gaagtggctc
      601 cccggcaccg acgtgccggt catcgagtag cggagccgag cgccgacggc cacgccccgg
      661 gaccccgggc ccgtccacgc gaccgtgggc ccgcacgcgg cgatgacgcc gcccccgccg
      721 cgcctcggac ggatgcggcg gaactcggcc ctgatcgcct cggtcagcac gttcgccgtg
      781 ctcggtgcga tcgtgttcgt gttcgtcacg gcgccagggg ccaagaagtt ccaggaggcg
      841 ttcctgagcc cgcaggaggc gctcgacagc gtccaccccg tctggacggg cttcgtgatc
      901 aacctccagc tcatggttgc cggcgaggcg ctcgtcctcg tgttcgcgct tctgatcgcg
      961 gtcgtgcgcg gcctgccggg cccggcggcg ttcccgctgc ggctgctcgc gatcgcctac
     1021 acggacttct tccgcggcgt gccgctgatc ctcgtcgtct tcgcgatcgg cctcgggatc
     1081 ccggcgctcg gcatcgacat cctctacacc gacaacttct tcgtctacgg gctcgtctcg
     1141 ctcgtgctcg tctactccgc gtacgtggcc gaggtctacc gctccgggat cgagtccgtg
     1201 cacgagagcc aggtcgcggc agcgcgctcg ctcagcctca cgcacttcca gtcccttcgc
     1261 cacgtcgtcg tcccgcaggc ggtccggcgc gtcgtccccc cgctcctgaa cgacttcatc
     1321 ggcctgcaga aggacaccgc gctcgtcgcg ttcgtcggcg tcctcgacgc attccaccag
     1381 gcccaggacc ggatggcgat cacctacaac ttcacggccc tgatggtggc cgcgctcttc
     1441 ttcgtcgccc tcacgatccc gctcgcccgc gtcaccgact ggctcgtcga gcgcgaccgc
     1501 cgcaagatga gggcgctcgg gggatgagcg ccaacggctc ggccctcctc ctcgaggacg
     1561 tctgcaagcg cttcggcgcg aacgaggtgt tgcgcggaat cgacctcgac gtcgggaagc
     1621 acgaggtcgt ctgcctgatc ggcgcgtcgg ggtccggcaa gtccacgctc ctcaagtgcg
     1681 tgaacctgac cgagaaggtg tcgtcgggtc ggatcgtggt cgagggtcac gagatcaccg
     1741 cccgcggcgt ggacgtgaac aggatccgcc ggaacatcgg gatcgtcttc caggcgttca
     1801 acctcttccc gcacatgacc gtgctccgga acgtgacgct cgcgccgcgc aaggtgctcg
     1861 gaaagtcgcg tcaggaggcc gaggctgagg ggctcgagct cctcgagcgg ttcggcctct
     1921 cggacaagcg tgacgagtat cccgaccggc tctcgggcgg tcagcagcag cgggcggcga
     1981 tcgtccgcgc gctcgcgatg cggccgaaga tcctgctcct cgacgaggtg acgagcgccc
     2041 tcgaccccga gctcgtcgcc gaggtcctct ccgtgatccg cgagctggcg gagtccggga
     2101 tgaccatggt gatcgcgacg cacgagatgg gcttcgcccg cgacatcgcc gaccgcgt
//
LOCUS       DASQDO010000268         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_395109, whole genome shotgun sequence.
ACCESSION   DASQDO010000268 DASQDO010000000
VERSION     DASQDO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395109"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VEV43_07555"
     CDS             <1..842
                     /locus_tag="VEV43_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP23411.1"
                     /translation="VQDVHPDVAIEVGAIRGPLIPVFKRLERWIYDHADAVTVLSEDL
                     CANVGAKTRDPSKVKVIPNFVDTGAIVPADRENAYRRRHGLTGKTVVMYAGNVGLSQS
                     LDLVVTAAAALAHEQDLVFVINGSGTDRKELEQKARGLPNVVFVDPQGDLAEVLAAAD
                     VHLVPLKKGLARASVPSKTYSILAAGRPLLASVDVGSEVARVVEQSGAGVAVPPDDAE
                     ALTKAVRRLVENPAEARAMGEAGRRWIEGWPSPTQIAEAYEALFEELLSSGGRGSSGT
                     RGW"
     gene            910..1386
                     /locus_tag="VEV43_07560"
     CDS             910..1386
                     /locus_tag="VEV43_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYP23412.1"
                     /translation="MIARRAALPPFQVLLDSYKDDVYRFLCGLLGPADADDCFQETFL
                     AALRAYPSLRNGDNLKSWLLTIAHRKVIDRARATRPTAPLDGAPHPAHDAPAEPDPGL
                     WAEVDALPGKQRAAVVLRFVNDMAYRDIGAVLDCSEAAARRSTHEGVKRLREVIER"
     gene            1383..1973
                     /locus_tag="VEV43_07565"
     CDS             1383..1973
                     /locus_tag="VEV43_07565"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010840264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYP23413.1"
                     /translation="MNEIKARLAARAAPAPATDGLIERAAEEGLVDVAYDAVDSPIGT
                     LLVATTDEGLVRISFHSEAEDLVLEELARRVSPRVVKAPRKVAQVARELDEYFAGRRK
                     DFDVPLDWRLVGPYARKVLTATAAIPFGQVSTYREVARKAGNPAASRAAGNALGSNPI
                     PVVVPCHRVLRTGGGLGGYGGGLDVKETLLRLEGVI"
     gene            2034..>2297
                     /locus_tag="VEV43_07570"
     CDS             2034..>2297
                     /locus_tag="VEV43_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP23414.1"
                     /translation="MSELVAEAESVGRTYDASGRFVQALVDVSASFHGGRVAAVSGPS
                     GSGKSTLLRILAGLDVPTEGEVTVCGTALSRLGPRARRKLRRTD"
BASE COUNT          347 a          829 c          804 g          317 t
ORIGIN      
        1 acgtgcagga cgtccacccg gacgtcgcta tcgaggtggg cgcgatacgg ggaccgctga
       61 tccccgtctt caagaggctc gaacgctgga tctacgacca cgccgacgcg gtgaccgtcc
      121 tgtccgagga cctgtgcgcg aacgtcggtg cgaagacgcg cgacccctcg aaggtgaagg
      181 tgatcccgaa cttcgtggac accggcgcga tcgtgccggc cgaccgcgag aacgcgtacc
      241 ggagacgaca cgggctcacc ggaaagaccg tcgtcatgta cgccggcaac gtcgggctct
      301 cgcagtcgct cgacctggtc gtcacggccg ccgcagcgct cgcgcacgag caggacctcg
      361 tcttcgtgat caacggctcg ggcaccgacc ggaaggagct cgaacagaag gcccgcggcc
      421 ttcccaacgt cgtgttcgtc gacccgcagg gggatctagc cgaggtgctc gccgcggccg
      481 acgtgcacct cgtcccgctg aagaagggcc tggcgcgcgc gagcgtcccg tcgaagacgt
      541 actcgatcct ggccgccggc cgccccctgc tggcgagcgt cgacgtgggc agcgaggtag
      601 cccgggtcgt cgagcagtcc ggcgcgggcg tggcggtccc cccggacgac gccgaggccc
      661 tcacgaaggc ggtgcggcgc ttggtcgaga acccggcgga ggcccgggcg atgggcgagg
      721 ccggccggcg ctggatcgag ggctggcctt caccgaccca gatcgccgag gcctacgaag
      781 ccctgttcga ggaactgctc tcgtccgggg gccgtggttc gtctggtacg cgtggttggt
      841 gaggttggac tcgcaacctc ggcaacctca ccaacctcac gaaccagagc ccccggacgt
      901 tcaatacacg tgatcgcccg ccgcgctgcg ctgcccccgt tccaggtcct cctcgactcc
      961 tacaaggacg acgtctaccg cttcctgtgc gggctcctcg gcccggccga cgccgacgac
     1021 tgcttccagg agaccttcct cgccgctcta cgggcgtatc cctcgttgcg caacggcgac
     1081 aacctcaagt cgtggttgct gacgatcgcc catcgcaagg tcatcgaccg ggcccgggcg
     1141 acccggccga cggctcccct cgacggcgcc ccccacccgg cccacgacgc tcccgccgag
     1201 cccgaccccg gcctgtgggc cgaggtcgac gctctgcccg gcaagcagcg agctgcggtc
     1261 gtcctccgtt tcgtcaacga catggcctac cgggacatcg gcgcggtcct cgactgcagc
     1321 gaagccgccg cccggcgcag cacgcacgag ggcgtgaaac gactacgaga ggtgatcgaa
     1381 cgatgaacga gatcaaggcg cggctcgcgg cacgagcggc cccggccccc gcgacggacg
     1441 ggctgatcga gcgagcggcc gaggagggcc tggtcgacgt cgcctacgac gcggtggact
     1501 cgccgatcgg gacgttgctg gtggcgacga cggacgaggg cctcgtgcgg atcagcttcc
     1561 attcggaggc cgaggacctc gtgctcgagg agctcgcccg cagggtgtcg ccgcgggtcg
     1621 tcaaggcgcc ccgcaaggtg gcgcaggtgg cccgggagct cgacgagtac ttcgccgggc
     1681 ggcgcaagga cttcgacgtc ccgctcgact ggaggctagt cggcccctac gcccgcaagg
     1741 tgctcacggc gaccgcggcc atcccgttcg gccaggtctc gacgtaccgc gaggtagcgc
     1801 gcaaggccgg gaaccccgcg gcgagccggg cggcaggcaa cgccctcggg tccaacccga
     1861 tcccggtggt ggtcccgtgc caccgggtcc tgcggacggg cgggggcctc ggcggttacg
     1921 gcggaggcct cgatgtcaaa gagacgctgc tccggctcga aggtgtcatc tgaacgccaa
     1981 tatcccccac ccgcgtccgc gggtagcccc ctcttacgga gtaagaagtc ccggtgagcg
     2041 agctcgtcgc cgaggcggag tccgtcggcc ggacgtacga cgcctcgggg cgcttcgtcc
     2101 aggccctcgt cgacgtgagc gcgtccttcc acgggggccg cgtcgcggcc gtctcggggc
     2161 cttcgggtag cggcaagtcg accctgctcc gcatcctcgc cgggctcgac gtcccgaccg
     2221 agggcgaggt caccgtgtgc gggaccgcgt tgtcgaggct cggcccccgc gcccggcgca
     2281 agctcaggcg caccgac
//
LOCUS       DASQDO010000269         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1068379, whole genome shotgun sequence.
ACCESSION   DASQDO010000269 DASQDO010000000
VERSION     DASQDO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068379"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /gene="der"
                     /locus_tag="VEV43_07575"
     CDS             complement(<1..1052)
                     /gene="der"
                     /locus_tag="VEV43_07575"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HYP23415.1"
                     /translation="MPPLPVVAIVGRPNVGKSSLLNALVGKRISIVQDMPGVTRDRIS
                     TPLKVGDRYVELMDTGGYGFVDPDQLTDHIKHQIELAMTSAQLVLFIVDCQDGLTSAD
                     ENIASLLRRKGITTVLVANKADGPKADVILGDFARLGLGTPVGVSAMNDRNLDQLTSA
                     IARNLDLSHAPTSIPEPQIMVAIVGKRNAGKSTLVNAFAEIYEGHGDRVIVSEVPGTT
                     RDSVDVRFEKDGKALVVIDTAGVRKKRHMVTNDVEFYSFHRAERSIRRADVVLMLVDA
                     TEPVSEPDKKLARYIAEEHKPVIVVVNKWDRVLENAKRQAEEKGTGFSPDLLMEEFRE
                     YLDQELKNLDYAPVAF"
     gene            1252..1692
                     /locus_tag="VEV43_07580"
     CDS             1252..1692
                     /locus_tag="VEV43_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYP23416.1"
                     /translation="MATVQDVLSTKSSKVLTTRRDATVLEAINKMNEHRVGALVVCDG
                     DHVVGMFTERDVLRKVAVCDRPPTAMLVEEVMTHDVVCTGPKEDLDEVRTVMKNRRFR
                     HLPVCDDLTGGLLGLISIGDLNAHDASHREATIHFLNEYIYGRV"
BASE COUNT          260 a          530 c          602 g          313 t
ORIGIN      
        1 atgaaggcga cgggcgcgta gtccagattc ttcagctcct gatcgaggta ctcgcggaac
       61 tcttccatca gcaggtccgg gctgaagccg gtccccttct cctccgcctg ccgcttcgcg
      121 ttctccagca cccggtccca cttgttcacg acgacgatga ccggcttgtg ctcctcggcg
      181 atgtagcggg cgagcttctt gtccggctcg ctcaccggct cggtcgcgtc gaccagcatc
      241 agtaccacgt cggcccggcg gatcgagcgc tcggcgcggt ggaagctgta gaactcgacg
      301 tcgttggtca ccatgtgccg cttcttgcgg acgccggccg tgtcgatgac gacgagggcc
      361 ttgccgtcct tctcgaagcg gacgtcgacg ctgtcgcgcg tggtgccggg cacctcggag
      421 acgatgacgc ggtctccgtg cccctcgtag atctcggcga acgcgttgac gagcgtgctc
      481 ttgccggcgt tgcgcttgcc gacgatggcg accatgatct gcggctcggg gatgctcgtc
      541 ggcgcgtgcg agaggtcgag gttgcgggcg atggcgctcg tgagctggtc gaggttgcgg
      601 tcgttcatgg ccgacacgcc taccggcgtg ccgaggccga gccgggcgaa gtcgcccagg
      661 atgacgtcgg ccttggggcc gtcggccttg ttggcgacga ggaccgtcgt gatgcccttg
      721 cgccggagga ggctggcgat gttctcgtcc gcgctcgtca ggccgtcctg gcagtccacg
      781 atgaacagca cgagctgggc gctcgtcatc gccagctcga tctgatgttt gatgtggtcg
      841 gtgagctggt cggggtcgac gaacccgtac ccgccggtgt ccatgagttc gacgtagcgg
      901 tcgccgacct tcaggggcgt ggagatgcgg tcgcgggtga cgccgggcat gtcctgcacg
      961 atgctgatcc gcttgccgac gagggcgttg agcaggctgg acttgccgac gttgggtcgg
     1021 ccgacgatcg cgacgacggg gagggggggc atggcgggcg attctagtcc gatagcgggc
     1081 aaatgcgact cgaccaaatc caagcggggc cttgctttgg gaatgttttc acgatgttgc
     1141 gtggagcagc tcaaatgtgg tactaaccgg cccgtcgggc tgccgaggcg cgttcacgtc
     1201 accgggttga ttcacctccc cgcacgtcgg agcgggcgaa aggaagcggt catggccacc
     1261 gtccaagacg tcctgtcgac caagtcgtcc aaggtcctca ccacccgtcg cgacgcgacg
     1321 gtgctcgagg cgatcaacaa gatgaacgag caccgcgtcg gcgcgctggt ggtgtgcgac
     1381 ggcgatcacg tcgtcggcat gttcaccgag cgtgacgtgc tgcggaaggt cgccgtctgc
     1441 gaccgcccgc cgacggcgat gctggtcgag gaggtcatga cgcacgacgt ggtctgcacg
     1501 ggccccaagg aagacctgga cgaggtgcgg acggtcatga agaaccgccg cttccgccac
     1561 ctgccggtct gcgatgacct gacgggcggc ctgctcgggc tgatctcgat cggcgacctg
     1621 aacgcccacg atgcctccca ccgcgaggcg acgatccact tcctcaacga atacatctac
     1681 gggcgggtgt aaggggcggg ggcgg
//
LOCUS       DASQDO010000270         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_517106, whole genome shotgun sequence.
ACCESSION   DASQDO010000270 DASQDO010000000
VERSION     DASQDO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517106"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VEV43_07585"
     CDS             <1..1773
                     /locus_tag="VEV43_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYP23417.1"
                     /translation="MRGALVPREASGRRAHHVLTLINAGNASLEPAVRVVDENNALVA
                     RVDPAFPAFPPGRSQKVDVYVRPRRRKWVGAAQTYRYRVVAHEDGVAPVELDASLTQR
                     AFVPGWAVRLAALAAPLAAAVIAFVMLWTVVPEVEGLPAAAAVEDLEEAGLESTVVRR
                     HSATVEEGTVLGSEPEGGIRVREGSKVKLFVSSGPRPVPIPNVVGLDAASARLELSSM
                     GLAIEEKAEESEEVEPGLVVATRPVANLLVPPGSKVQMIVAAPPEGKGGKGAEDEGSG
                     SGSDGGGAGSEEDEAGGEDEERGRGGRAPYWTSSVASNDFDGDGAADLALFRPSTGAW
                     HVRFGPGRVRTVDWARQRGNDVPVPGDYDGDGSTDFATWRPSDATWYVRYAGSKGHEV
                     AELGRPGDVPVPGDYDGDGRTDMAAFGADDGRWRIRPSSGDGSGVRDVEWGDRSLGDL
                     PVPGDYDGDGRTDLAYYRASTGRWRVAFGAGGTEAVRWGDPLEGDVPVPGDYDGDGTH
                     DYAVWRPPTGEWRIRPSGRGDSLFVTWGRRDLLDVPVPGDYTGDGTFDLAVWRESTRA
                     WRIRPAEGTKRRIHYWGRAGDVPL"
     gene            1917..2081
                     /locus_tag="VEV43_07590"
     CDS             1917..2081
                     /locus_tag="VEV43_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23418.1"
                     /translation="MEPKMRELFVEELADVRGGARGGVPGKPDTVTTMACCEEGPFGC
                     CGETVETQPI"
     gene            2218..2373
                     /locus_tag="VEV43_07595"
     CDS             2218..2373
                     /locus_tag="VEV43_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23419.1"
                     /translation="MRELFIDELTDVRGGFHGGLDYSTEAFCEEGPFDGCCGWWERIE
                     DLISITP"
BASE COUNT          380 a          834 c          977 g          329 t
ORIGIN      
        1 atgcgcggcg cgctcgtccc ccgcgaagcc tccggccgcc gcgcacacca cgtgctgacg
       61 ctgatcaatg ccggcaacgc gtcgctcgag cccgccgtgc gcgtcgtcga cgagaacaac
      121 gccctcgtcg cccgggtcga ccccgcgttc ccggccttcc cgccggggcg cagccagaag
      181 gtcgacgtgt acgtacggcc ccggcgccgc aaatgggtcg gggccgcgca gacgtatcgg
      241 taccgggtcg tcgcgcacga ggacggcgtc gcgccggtcg agctcgacgc gtcgctgacc
      301 cagcgtgcct tcgtgccggg ctgggcggtg cggctcgcgg cgctcgcggc ccccctggca
      361 gcggcggtga tcgcgttcgt gatgctgtgg acggtcgtcc ccgaggtcga agggctcccg
      421 gccgccgcgg ccgtcgagga cctcgaagag gcgggtctcg agagcacggt cgtacgccgc
      481 cacagcgcca ccgtcgaaga agggaccgtc ctcggcagcg agcccgaggg cggcatccgc
      541 gtgcgcgagg ggtcgaaggt caagctgttc gtctcgtccg gcccgcggcc ggtccccata
      601 ccgaacgtcg tcgggctcga cgcggccagc gcgcggctcg agctctcgtc gatggggctc
      661 gcgatcgagg agaaggccga agagagcgaa gaggtcgagc ccgggctcgt cgtcgcgacg
      721 cgcccggtcg ccaacctcct cgtcccgccg gggtcgaagg tgcagatgat cgtcgccgcg
      781 ccgccggagg gaaagggcgg caagggggcg gaagacgaag ggtccggctc cggctcggac
      841 ggcggcggag ccggctccga ggaagacgag gccggcggtg aggacgagga gcgggggcgc
      901 ggagggcgtg ctccgtactg gacctcgtcg gtcgcgtcga acgacttcga cggtgacggt
      961 gcggccgatc tcgcgctctt ccgtccgagc acgggggcgt ggcacgtcag gttcgggccg
     1021 ggccgggtgc gcaccgtgga ctgggcccgc cagcgcggga acgacgtccc ggtcccgggc
     1081 gactacgacg gggacggaag caccgacttc gcgacctggc gcccgagcga cgcgacctgg
     1141 tacgtccgct acgccgggag caagggacac gaggtcgcgg agctcggccg gcccggcgac
     1201 gtcccggtcc ccggcgacta cgacggcgac ggcaggaccg acatggcggc cttcggcgcg
     1261 gacgacggac gctggcgcat ccggccgagc agcggcgacg gctcgggcgt ccgcgacgtc
     1321 gagtggggcg atcggtccct cggcgacctg cccgtccccg gggactacga cggcgacggt
     1381 aggacggacc tcgcctacta ccgggcgtcg accggccgct ggagggtcgc gttcggtgcg
     1441 ggcgggaccg aagcggtccg gtggggcgac ccgctcgagg gcgacgtccc cgtgccgggc
     1501 gactacgacg gcgacgggac ccacgactac gccgtctggc gccccccgac cggcgagtgg
     1561 cggatccgcc cgagcggacg gggcgacagc ctgttcgtca cctgggggcg cagggacctg
     1621 ctcgacgtcc ccgtgcccgg cgactacacg ggcgacggca ccttcgacct cgccgtgtgg
     1681 cgggagtcga cgagggcctg gaggatcagg ccggccgagg ggacgaagcg tcgcatccac
     1741 tactggggcc gagcgggcga cgtccctctg tgaggccgcc gagcggacta gtccgcgccc
     1801 tcccccatcc gggggggccg ggtggtttac cgggtcattt ctcaactatc cccttgagcg
     1861 cgaggtgaac cttcctcgac aatccgccga gtcaaccgac tgggagggga ttcgagatgg
     1921 agcccaagat gcgtgagctg ttcgtagagg agctcgccga cgtgcgaggc ggggcccgcg
     1981 gcggcgtgcc ggggaagccc gacaccgtaa cgacgatggc ctgttgtgag gaaggcccgt
     2041 tcggctgctg cggcgagacc gtggagacgc agccgatcta gtagtcccgc cgtctcgacc
     2101 tggcgccgtc cctgaggggg cggcgccttt tcgcgcgttt accaatgact tgagcaatcc
     2161 gaggaggcgg gggacaatac cgcccgattc gtacgggatg ggtgcgaaag gagcgacatg
     2221 cgcgagctgt tcatcgacga gctcaccgac gtccgcggcg ggttccacgg cggcctcgac
     2281 tattcgaccg aggccttctg tgaagagggc ccgttcgacg gctgctgtgg ctggtgggag
     2341 cggatcgagg acctgatctc gatcacgccg tagaggggaa tcgggaggcg aactagaaac
     2401 aactcgcggg caaaccgaga ggaggaatcg atgggcaagg agatcgtgcg ggagctgttc
     2461 gtggacgagc tggcgagcgt gacgggcggc aaggagtgtc cggactgctg gcagaccacg
//
LOCUS       DASQDO010000271         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_333584, whole genome shotgun sequence.
ACCESSION   DASQDO010000271 DASQDO010000000
VERSION     DASQDO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333584"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1331)
                     /locus_tag="VEV43_07600"
     CDS             complement(9..1331)
                     /locus_tag="VEV43_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYP23420.1"
                     /translation="MLDRLLELTNALRDAGVPVSTSEAIDAARSLEHVPVESKAALKL
                     ALSSTLVKTPAHSAAFDALFTIYFEAGLQERPAEDVEDPADLRREIESALTSGDGAGL
                     GDLASRAVETFGRVENSPSDSLYFEYPVTRALDLDGLGQRGVDETDPLQRRLLQDAFD
                     ERVRRFRSAVTDEVRKRVVRRRGPEAVARYAVPPVLEDLELMTAAADEMAALRRAVRP
                     LARRLAARMAMKRRRAARGRLDVRRTVRHSLSSGGVPVETFFRARPPHRPELFVLCDV
                     SSSVASFSRFSLMLVHALSSEFQRVRSFAFVDTVDEVTRFFDDEDFARAAERMRAEAK
                     VVRIDSNSNYGASFEAFWERFGKELGPKSTVLVLGDARNNNRPPREHNLRKIAERARR
                     TIWLNPEALVYWDSGDSIASLYAAQVDEMVEVRNLRQLERFVEGKLTA"
     gene            complement(1331..2206)
                     /locus_tag="VEV43_07605"
     CDS             complement(1331..2206)
                     /locus_tag="VEV43_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYP23421.1"
                     /translation="MSSPRFTSIEDVSSRLEAGGYLAGEDVATVVYLAGRLGKPLLVE
                     GPAGAGKTELAKTVAAALGLRLVRLQCYEGVDETKVLYEWNYRKQLLRIQAGQGAGWD
                     ELSDDVFGEEFLLERPVLQALRSEEPVVLLIDEVDKLDVEAEALLLEVLSDWQVSIPE
                     MGTVVARGEPFVVLTSNAVRELSEALKRRCLYLYLDYPDLEREKAIVLRRVPGLGEAL
                     ADAVVRIVRSIRELELKKPPSIAETLDWARTLLELSITTLDGEALRRTLPVLVKFRSD
                     IEKVTGELKIAPPEG"
     gene            2353..>3108
                     /locus_tag="VEV43_07610"
     CDS             2353..>3108
                     /locus_tag="VEV43_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP23422.1"
                     /translation="MFKKALLVAMLPALIWAPPAASDDGGPGDPMLSQQWGLTRIRAN
                     EAWPLATGEGITIAVVDSGVDLTHPDLAANVDVERDMDFVDGKADTDGAQDTSGHGTH
                     VAGIAAAVTNNGVGVAGVAPKAVILPVRVLGAEQDRTARIAAGVRYAADEGAHVINLS
                     LSYSPPGHVDSITGNMKPVHDAISYALGKGAVVVASSGNESLPLCAEPAAVNGVLCVG
                     AVDRSERPAYYTNFESRRAKRYVVAPGGDQGLTC"
BASE COUNT          473 a         1097 c         1080 g          458 t
ORIGIN      
        1 tacgccggct aggccgtcag cttcccctcg acgaaccgtt ccagctggcg gaggttgcgc
       61 acctcgacca tctcgtcgac ctgggccgcg tagaggctcg cgatcgagtc gccgctgtcc
      121 cagtacacga gcgcttcggg attgagccag atcgtgcgcc gcgcccgctc ggcgatcttg
      181 cgcaggttgt gctcgcgcgg gggccggttg ttgttgcgcg cgtcgccgag cacgagcacg
      241 gtcgacttcg ggcccagctc cttgccgaag cgctcccaga acgcctcgaa cgaggcgccg
      301 tagttcgagt tcgagtcgat gcgtacgacc ttggcctcgg cgcgcatcct ctccgcggcc
      361 cgggcgaagt cctcgtcgtc gaagaacctc gtcacctcgt cgacggtgtc gacgaacgcg
      421 aacgagcgca cgcgctggaa ctccgacgag agcgcgtgga cgagcatgag cgagaagcgc
      481 gagaagctgg ccaccgagct cgacacgtcg cacagcacga agagctcggg gcggtgtggg
      541 ggccgcgcgc ggaagaacgt ctcgacggga acgccgccgg aggacagcga gtgccgcacg
      601 gtcctgcgta cgtcgaggcg accgcgggcc gcgcgcctgc gcttcatcgc catccgggcc
      661 gcgagccggc gggcgagcgg cctgacggca cggcgtagcg cggccatctc gtcggcggcc
      721 gcggtcatca gctcgaggtc ttccagcacg ggagggaccg cgtagcgagc gacggcctcc
      781 ggcccccgcc tccgcacgac cctcttgcgc acctcgtcgg tcaccgccga ccggaaccgc
      841 cgcaccctct cgtcgaacgc gtcctggagc aggcgccgct gtagggggtc ggtctcgtcc
      901 accccccgct gccccagccc gtcgaggtcg agcgcgcgcg tcaccgggta ctcgaaatag
      961 aggctgtccg aagggctgtt ctcgacgcgc ccgaaggtct cgacggcgcg gctcgcgagg
     1021 tcgccgaggc ccgctccgtc ccccgaggtg agcgcactct cgatctcgcg acgcagatcc
     1081 gccggatctt cgacgtcctc cgccggcctc tcctgcaacc ccgcttcgaa gtagatcgtg
     1141 aacagcgcgt cgaaggcggc gctatgcgcg ggagtcttca cgagcgtcga cgacagcgcg
     1201 agcttcagag cggccttgct ctcgaccggg acgtgctcca aggaccgggc cgcgtcgatc
     1261 gcctcgctcg tcgacaccgg cacgcccgcg tcgcgcaggg cattggtgag ctcgagcagg
     1321 cgatcgagca ctacccctcc ggcggtgcga tcttcagctc gccggtcacc ttctcgatgt
     1381 cgctgcggaa cttcacgagg accgggagcg tgcggcgcag cgcctcgccg tcaagcgtcg
     1441 tgatgctgag ttcgagcaac gtccgcgccc agtcgagcgt ctccgcgatc gaaggcggct
     1501 tcttgagctc gagctcgcgg atgctgcgca ctatccgcac gaccgcgtcg gcgagcgctt
     1561 cgccgaggcc gggcacgcgg cgcagcacga tcgccttctc gcgttcgagg tcgggatagt
     1621 cgaggtagag gtagaggcac cgtcgcttga gcgcctccga gagctcgcgc acggcgttcg
     1681 acgtcaggac gacgaagggc tcgccgcgcg ctacgacggt tcccatctcg gggatcgaga
     1741 cctgccagtc cgacaggacc tcgaggagca gcgcctcggc ctcgacgtcg agcttgtcga
     1801 cctcgtcgat cagcagcacg acgggctcct cggaccgcag cgcctggagg accggacgct
     1861 cgagcaggaa ctcctcgccg aagacgtcgt ccgagagctc gtcccagccc gcgccctggc
     1921 cggcctggat ccgcaagagc tgcttgcggt agttccactc gtagaggacc ttcgtctcgt
     1981 cgacgccctc gtagcactgg aggcgcacga ggcgcagccc gagggcggca gcgacggtct
     2041 tggccagctc ggtcttgccg gctccggcgg gcccctcgac gagcagcggc ttgccgaggc
     2101 ggccggcgag gtagacgacg gtcgcgacgt cctcgcccgc gaggtagccg cccgcttcga
     2161 gccgggagga gacgtcctcg atgctggtga accggggcga gctcaagatg gcctcagcct
     2221 aggcccccgg gggcgcgacc ctgggccggt cgtggccgtt aaggtccaac tggagggtct
     2281 ttccagcaag gcaggagagg ccccctccga ggcgaaaatc cactgaggaa cctttcgagc
     2341 aaaaggccct gcgtgttcaa gaaagctctt ttagtcgcga tgctgccggc cctgatctgg
     2401 gcccctcccg cggcgtccga cgacgggggg ccgggcgacc ccatgctgag ccagcagtgg
     2461 ggcctgaccc gcatccgggc aaacgaggcg tggccgctcg caaccggcga ggggatcacg
     2521 atcgcggtcg tggactccgg ggtcgacctg acccatccgg acctcgccgc caacgtcgac
     2581 gtcgagcgcg acatggactt cgtcgacggc aaggccgaca ccgacggggc tcaggacacc
     2641 tccggtcacg gcacgcacgt cgccggcatc gccgcggcgg tcactaacaa cggcgtcggt
     2701 gtcgcagggg tcgctccgaa ggccgtcatc ctcccggtgc gcgtgctggg ggccgagcag
     2761 gatcgcaccg cccgcatcgc tgccggtgtc cggtatgccg cggacgaggg cgcgcacgtg
     2821 atcaacctct cgctgtcgta ctcgccgccg ggacacgtgg actcgatcac cggcaacatg
     2881 aagccggtgc acgacgcgat ctcgtacgcg ctcggcaagg gagcggtcgt ggtcgcgtcg
     2941 tccggcaacg agtcgctgcc gctgtgcgcc gagcccgccg cggtcaacgg cgtcctgtgc
     3001 gtcggcgcgg tggatcgttc cgagcgcccc gcctactaca cgaacttcga gtcgcgccgt
     3061 gcgaagcggt acgtcgtcgc ccccggggga gatcaggggc tgacgtgc
//
LOCUS       DASQDO010000272         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_884551, whole genome shotgun sequence.
ACCESSION   DASQDO010000272 DASQDO010000000
VERSION     DASQDO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884551"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VEV43_07615"
     CDS             complement(<1..638)
                     /locus_tag="VEV43_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYP23423.1"
                     /translation="MQLGLALPHYDTSYRNEPVAWPSVRDCALAADRSGFSSVWVSDH
                     LFLDWGKYGGPATRQGSLECWTTMTALAAATSKVRVGSLTLCNDLRSPALVAKMAASL
                     DLLSGGRLDVGLGAGWYEPEYRAAGIDFDSAGTRIRRLGEAVEIVGRLLAGEELTFDG
                     RHYQVQEAICLPRPAQSPRPPVWVGGKGDLLLRTAARVADGWNFSWIGSFET"
     gene            662..1126
                     /locus_tag="VEV43_07620"
     CDS             662..1126
                     /locus_tag="VEV43_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYP23424.1"
                     /translation="MKVSDIMTQATVTDASDDTLAEASSKMWRQQTGSLLVMEGTRLV
                     GIVTERDVLRTIGEGHDPKAVSLRDVMTADVVTVKSDLSVREAAQIMFDKWFRHLPVV
                     DTDGTVVGIVSLRDLNGLVAQGMEEPDRLEHLTGHKLVRDKRLERVEAGDLD"
     gene            1154..1423
                     /locus_tag="VEV43_07625"
     CDS             1154..1423
                     /locus_tag="VEV43_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23425.1"
                     /translation="MGSIRRHPFLTLIFVVGSLPVVFLLVVEAAQHTSACRRWRAEVD
                     ERSTIREPPAWVEYDRGDMPLDEYYGGVRDHVAHELREGRPLFCK"
     gene            complement(1454..2377)
                     /locus_tag="VEV43_07630"
     CDS             complement(1454..2377)
                     /locus_tag="VEV43_07630"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYP23426.1"
                     /translation="MRWPDIDRYLRSLVAGLKEVFREDDWSLAQTIVDGLAERRARTG
                     ERLDQAVQRALVAGRYAVRSWFVNGDQELCDDLLLDALHECVFRFSESFQGIHLESED
                     QRVQSRIIKSLVMALEARDPYTKGHSISVALLSQKIAQLLDPTMDADRVYLAGLLHDV
                     GKVGIPDSILLKEGPLDDDEWEVMRSHPAKGADILSPIRLYPDVVAGVYSHHENYDGT
                     GYPTGSSGDEIPMIGRIIRVVDSFDAMTTTRVFRKSRSMDDALEELMRRRAVYYDPEI
                     VDSLVEIVETPGIMRELGLASLQIEMGEAAV"
     gene            complement(2626..>2822)
                     /locus_tag="VEV43_07635"
     CDS             complement(2626..>2822)
                     /locus_tag="VEV43_07635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23427.1"
                     /translation="KLFASEACHEITLKSLRVHGGYGYSKEFTVERLYRDAPFLLIGE
                     GTSEIQKTVIARRLLERYKI"
BASE COUNT          473 a          930 c          949 g          470 t
ORIGIN      
        1 taggtctcga aggagccgat ccacgagaag ttccacccgt ctgcgacgcg ggccgcggtc
       61 ctcaggagga ggtcgccctt gccaccgacc cagaccgggg gccgcgggct ctgcgccggc
      121 cgcgggaggc agatcgcctc ctgcacctga taatgccggc cgtcgaacgt cagctcctcg
      181 ccggcgagca ggcggccgac gatctcgacg gcctcgccga ggcgccggat cctcgtgcct
      241 gccgaatcga agtcgatccc cgccgcccgg tactccggct cgtaccagcc ggcgccgagg
      301 ccgacgtcga ggcgcccgcc ggagagcagg tcgaggctcg cggccatctt cgcgacgagg
      361 gccgggctgc ggaggtcgtt gcacagggtg agcgagccca ctcgcacctt cgaggtggcg
      421 gcggcgagcg cggtcatcgt cgtccagcac tcgagcgacc cctgccgcgt cgccgggccc
      481 ccgtacttgc cccagtcgag gaagagatgg tcggagaccc agacggacga gaaaccggag
      541 cggtcggcgg ccagtgcgca gtcacggacg gagggccacg ccacgggctc gttccggtag
      601 ctcgtgtcgt agtgcggaag cgctagcccg agttgcaccg gcgcagtcta gaatcagcgg
      661 cgtgaaggtc tcggacatca tgacgcaggc gactgtgacc gacgcttcgg acgacactct
      721 cgccgaggct tcgtcgaaga tgtggcgtca gcagacgggt tcgctgctcg tcatggaggg
      781 cacccggctc gtcggcatcg tgaccgagcg cgacgtcctg cgcacgatcg gcgagggcca
      841 cgacccgaag gcggtgagcc tgcgcgacgt gatgacggcg gacgtcgtga cggtgaagtc
      901 cgacctgtcg gtccgcgagg ccgcgcagat catgttcgac aagtggttcc gccacctccc
      961 ggtcgtcgac accgacggca cagtcgtcgg gatcgtgtcg ctgcgagacc tgaacggcct
     1021 ggtggcgcag ggcatggagg agccggacag gctcgagcac ctgacggggc acaagctggt
     1081 ccgcgacaag cgcctcgagc gcgtcgaagc cggagacctc gactagcgcc gcctccgctc
     1141 ctacgctgta cctgtgggga gcatccgcag gcatcctttc ctgacgctga tcttcgtcgt
     1201 cgggtcgctg ccggtcgttt tcctgctggt ggtggaggcc gcgcagcaca cctccgcctg
     1261 cagacgatgg cgagccgagg tcgacgaacg gtcgacgatc cgggagccgc cggcatgggt
     1321 cgagtacgac cgcggcgaca tgccgctcga cgagtactac ggaggcgtcc gggaccacgt
     1381 cgcgcacgag ctacgcgagg gccggcctct gttctgcaag tgagagaaga ccccccgcat
     1441 ccgtgaggtg tcgctagacg gccgcttcgc ccatctctat ctgtagcgac gccaggccca
     1501 gctcccgcat gatgcccggc gtctccacga tctccacgag cgagtccact atctcggggt
     1561 cgtagtagac cgcgcggcgg cgcatgagct cctccagcgc gtcgtccatc gagcggctct
     1621 tgcggaacac acgcgtggtc gtcatcgcgt cgaacgagtc gacgacgcgg atgatgcggc
     1681 cgatcatcgg gatctcgtcc ccggacgagc ccgtcgggta tcccgtcccg tcgtagttct
     1741 cgtggtgcga gtacacgccg gccacgacgt ccgggtacag ccggatcggg ctcaggatgt
     1801 ccgcgccctt cgccgggtgc gagcgcatga cctcccactc gtcgtcgtcc agagggccct
     1861 ctttgaggag gatgctgtcg gggatcccca ccttgcccac gtcgtggagc agccccgcca
     1921 ggtacacgcg gtcggcgtcc atcgtcgggt ccagtagctg cgcgatcttc tgcgacagca
     1981 gcgcgaccga gatcgagtgg cccttcgtgt aggggtcgcg ggcctcgagc gccatcacga
     2041 gcgacttgat gatccggctc tgcacccgct ggtcctcgga ctcgaggtgg atcccctgga
     2101 acgactccga gaagcggaac acgcactcgt ggagggcgtc gagcaggagg tcgtcgcaca
     2161 gctcctggtc gccgttgacg aaccaggacc gcacggcgta ccggccggct acgagggcgc
     2221 gctggacggc ctggtcgaga cgctcgccgg tccgggcccg gcgctcggcc agcccgtcca
     2281 cgatcgtctg cgccagggac cagtcgtctt cccggaagac ctctttgagc ccggcgacga
     2341 gcgaccggag ataccggtcg atgtcgggcc atcgcacccg agcgtccttg ccggcggtct
     2401 cgacccagga agcgacgatc tcgtcgcggt gcgtttcgag gacctcagca aggcgaaccc
     2461 gggccaatga cattcggggt tcggcagttg cgtggagggc tgcgtatcta ctggttcgca
     2521 atagctatgt cctgtcccca gaatggacta gtgcacagga cgtagttatc ccactacgct
     2581 atggcgctgt aaaccccgaa tttggcgttt tcctggaaat tcctgttaga ttttgtaccg
     2641 ttcgagaagt ctgcgagcaa tgaccgtttt ttggatttca gacgttccct caccaatcag
     2701 aagaaatggt gcatcacggt aaagacgctc aacagtgaac tctttggaat atccgtaccc
     2761 cccatggaca cggagggatt tgagcgtgat ctcgtggcaa gcctcggacg cgaacagctt
     2821 cg
//
LOCUS       DASQDO010000273         6022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1069245, whole genome shotgun sequence.
ACCESSION   DASQDO010000273 DASQDO010000000
VERSION     DASQDO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069245"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..836)
                     /locus_tag="VEV43_07640"
     CDS             complement(180..836)
                     /locus_tag="VEV43_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYP23428.1"
                     /translation="MTAVVAGITLLLGLALGSGAADVGSAATARARAQTAADAAALAA
                     VAESSPSGEGRPTEAAADYAERNGARLLRCWCDVGATAMQVLVSVDGVVADARAVMDP
                     ALLRPAAVGSTEGLHPGLAAAVDEIVTKSSGRVWVVSGFRLPARQAQLWAAALGKYGS
                     AEAADDWVAPPGRSNHEKGLAVDLGGDLEEAVRLVEELDLPLHRPLAHEPWHFELSRR
                     "
     gene            complement(833..1219)
                     /locus_tag="VEV43_07645"
     CDS             complement(833..1219)
                     /locus_tag="VEV43_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HYP23429.1"
                     /translation="MPVRRLQGQEGQATLELALCLPVLALVFAGVVEVALIGVDQVRV
                     WHAAREAARVSAVDSDAAAAREAAGSSGLDGIEVSVEPGAEERAPGRPTTVHVAYPYD
                     ARVPVVGEVFERFVLEADATTRIEQS"
     gene            complement(1219..1389)
                     /locus_tag="VEV43_07650"
     CDS             complement(1219..1389)
                     /locus_tag="VEV43_07650"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HYP23430.1"
                     /translation="MKAWLAEERGQTTAEYSLVLLVAGGVVGVFAAFVKSGALSGMFQ
                     TIVRSLVERAGG"
     gene            complement(1480..1941)
                     /locus_tag="VEV43_07655"
     CDS             complement(1480..1941)
                     /locus_tag="VEV43_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HYP23431.1"
                     /translation="MQRIVALALLLCLAACSNGGGDGARAEPSPSPSFPIATVLLDNG
                     DRSTLVIVEVAETAEQRERGLSERESLPEDEGMVFVFFEERDTGLSFGSDIPLSVAFF
                     DATGEIVGIVDAEECSDETCSTAPYMGALAVNQGAFDEWEISEGDHLQLTR"
     gene            complement(1951..2901)
                     /locus_tag="VEV43_07660"
     CDS             complement(1951..2901)
                     /locus_tag="VEV43_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYP23432.1"
                     /translation="MKLPWQRVRELPPDELARAIRSLAGSIRGGTPPHVALARWDAPG
                     VVAVVSRCVALAESPAAAFDRAEEYLGSAAPALARCFALQRATGASLPRLLERVAESL
                     ERHDATARSSRATTSGARLSGRLVAGLPLAFVPLTSGGNPVRGGGAGIALLVAGIALG
                     VAGLWWIGRLVPAPPGGDDGAAALADDLASALDAGAGLVPALEAAVTHPPRDLDDELA
                     RVARRVRLGSGWAEALAREGPHLRSLSEVLERSRTSGVPAARPLRDWANARRAEARTE
                     MQRALRRAPVLMVVPLTVCVLPSFALLAFGPFVLGAFGGA"
     gene            complement(2898..3884)
                     /locus_tag="VEV43_07665"
     CDS             complement(2898..3884)
                     /locus_tag="VEV43_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYP23433.1"
                     /translation="MLSRSLLDRVLDNDWLATLDPAERRLALREVVAPDKIAAVAEHV
                     DGYGPLAEVMARPGVTDVLVNGPREVWAEQDGTLTREAIEWPDADALRDFVDRLLARG
                     GGSVDASRPVADGRLPDGSRIHVVLPPIAPRGPLVSIRRFPSVRFTLDDLVRAEMLSA
                     ANAARLRDLVAARATVVVSGATGAGKTTLLNALLALVGTAERVVTIEETPELGPFSSH
                     VVSLVARPPNVEGSGAVGLDALVRASLRMRPDRIVVGEVRGREAAVALAAMATGHEGS
                     MLTLHARSAAEAPERLAELAETDVGRVRRAVDAFVHLSRRDGRRKLEEIEAR"
     gene            complement(3878..4729)
                     /locus_tag="VEV43_07670"
     CDS             complement(3878..4729)
                     /locus_tag="VEV43_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23434.1"
                     /translation="MTKVAIVSNDPAVRDAAATAFATAPPEWTVELRSDEPDDADAVV
                     YGSDVGAGDGIVFDRERPGEALRAVEARVGRGRVYAVVGAAGGVGVTSLAVHLAAAMA
                     PSGYAELAGASSRLGLPGDALTWLPRDDDRSLQGLPVAGGLRVLRAPDPCPSPEAFPL
                     REARASFGRLVLDLGSRRDLDAVLTSSSAAVLVTTPTRPAALAARSLTEAFPEARWIV
                     VVNRLGPGGQIMRGGLEQLLGRPIGIELPCCPALRDAEDEARLLAGSWRRWTRGVARL
                     AKALSSC"
     gene            4803..5609
                     /locus_tag="VEV43_07675"
     CDS             4803..5609
                     /locus_tag="VEV43_07675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYP23435.1"
                     /translation="MEAAFFDLDKTIIAKSSPLALGRSFFREGLIGRSFLLKSLYAQL
                     VFALMGADEAKMDRMRVEAAKLTAGWEQEKVRQVVAEVLEEVISPLIYAEALELIHDH
                     RAAGRLVCIVSSSPEEIVEPLANMLRANRFIATKPTIVDGKYTGDLDFYAYGPHKAAA
                     IEELADEMGIDLSGSFAYSDSVTDLPMLEVVGNPVAVNPDKALRKIALEKGWDVEAFR
                     NPVPLRSRLPQLRPPEVSVPGRFVAAAGVAGVAAVAWWWTRRSGSKAEIG"
     gene            5614..>6022
                     /locus_tag="VEV43_07680"
     CDS             5614..>6022
                     /locus_tag="VEV43_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23436.1"
                     /translation="MGRMRRVTWAALLAVALFAGLLSQSGSLAAPTEAELDAARERLM
                     ELEAEFETVVERYNLVHENLTSIQARIGVTELEVRRIERRMGVRREAAVDVARELYMG
                     GSNDALEAVLIADDIGDVESRLAYLRTSQQAQSE"
BASE COUNT          927 a         2274 c         2080 g          741 t
ORIGIN      
        1 cggtcagccg ctcgcgcagc ctcctctcgg atcgacacgc cacctctctc tctttcccgc
       61 cggtcaaaga gtgtcaagtt cgatcctagc tgagggtatc gacgagacat tgggcgtacc
      121 gcttacaact tccgaaacca tggtcaggcc agccgaggcg agcgcggcgc gtttctcact
      181 catcgacgcg acagctcgaa gtgccacggc tcgtgcgcga gcggccggtg cagcggcaag
      241 tcgagctcct cgaccagccg cacggcttcc tccaggtcgc cgcccaggtc gaccgcgaga
      301 cccttctcgt ggttcgacct gcccggcggc gcgacccagt cgtcggcggc ctcggcactt
      361 ccgtacttgc ccagcgcggc ggcccacaac tgcgcctgac gcgccggcaa cctgaagccg
      421 gagacgaccc agaccctgcc gctcgacttc gtcacgatct cgtcgaccgc ggcggcgagg
      481 ccggggtgga ggccttcggt cgaaccgacc gcggcaggcc ggagcagggc cgggtccatc
      541 accgcgcgtg cgtccgcgac cacgccgtcg acggacacga gcacctgcat cgccgtggcg
      601 ccgacgtcgc accagcacct gagcagccgt gccccgttgc gctcggcgta gtccgcggcg
      661 gcctccgtcg ggcggccctc gccggacggc gacgactccg ccacggcggc gagggccgcc
      721 gcgtctgccg ccgtctgggc gcgcgcccgc gcggtcgccg cgctccccac gtcggccgcg
      781 cccgatccga gcgcgaggcc gagcaacagc gtgatccctg cgaccacggc cgtcacgact
      841 gctcgatcct ggtcgtggcg tccgcctcca ggacgaaccg ctcgaacacc tcacccacca
      901 ccgggacgcg cgcgtcgtac ggatacgcga cgtgcaccgt cgtcggccgt cccggcgcgc
      961 gctcctcggc ccccggctcg accgacacct cgatcccgtc gagccccgaa gaccccgcag
     1021 cctcgcgcgc cgccgcggcg tcggagtcga cggccgagac gcgcgccgcc tcgcgggccg
     1081 cgtgccacac gcgtacctgg tcgacgccga tcaacgcgac ctcgacgacc ccggcgaaca
     1141 cgagcgcgag caccggcagg cacagcgcga gctcgagcgt cgcctggcct tcctgtccct
     1201 gcagcctccg caccggcatc agcccccggc gcgctcgacg agcgagcgga cgatcgtctg
     1261 gaacatgccg gacagtgcgc cggacttcac gaacgcggcg aacacgccga cgacgccccc
     1321 ggcgacgagc aacaccaacg agtactccgc cgtcgtctgc ccgcgctctt ccgccaacca
     1381 cgccttcata gccacctccg atccaggccg gcccgatgcc ggcgccgggt tcgatcaaag
     1441 gcgaggccga gggcgccgta ggtggcgcgc gccacaggct taccgagtta gctggaggtg
     1501 atccccttcg gatatctccc actcgtcgaa cgcgccctgg ttcacggcga gcgcgcccat
     1561 gtagggggcc gtcgaacacg tctcgtccga gcactcctcc gcgtcgacga tcccgacgat
     1621 ctcgcccgtc gcgtcgaaga acgcgaccga gagcgggatg tcgctcccga aggaaagccc
     1681 ggtgtcgcgc tcctcgaaga agacgaacac catgccctcg tcctcgggca gggactctcg
     1741 ttcggacagg ccgcgctctc gttgctcggc cgtctcggcg acctcgacta ttacgagagt
     1801 cgacctgtcg ccgttgtcga ggagcacggt agcgataggg aaagaaggac tgggcgaggg
     1861 ctcggcccgc gcgccgtcgc cgccgccgtt cgagcaggcc gcgagacaga gcaggagcgc
     1921 gagcgcaacg atcctttgca tcgcgcgagg ttacgcgccc ccgaacgctc ccaggacgaa
     1981 cggtccgaac gcgagcaacg cgaacgacgg gagcacgcac acggtcagcg ggacgaccat
     2041 cagcacggga gctcgccgaa gcgcacgttg catctcggtg cgggcctcgg cgcgccgggc
     2101 gttcgcccag tcccgcaggg gccgggctgc cgggacgccc gacgtccgcg agcgttcgag
     2161 cacctcgctc agcgagcgca agtgcggtcc ctcgcgcgcc agggcctcgg cccacccgga
     2221 gccgagccgg acccggcgcg caacccgcgc cagctcgtcg tccagatcgc gtggagggtg
     2281 cgtgaccgcg gcctccagcg cagggaccag accggccccc gcgtccagcg cggacgcgag
     2341 gtcgtcggcc agcgcggccg ccccgtcgtc gcctcccggc ggtgccggca cgagcctccc
     2401 gatccaccac aggccggcga cgcccagcgc gatgcccgcg acgagcaggg cgatccccgc
     2461 acccccgccg cgcaccgggt tgccgcccga cgtcaacggc acgaacgcga gcgggaggcc
     2521 cgcgacgagg cgtccggaga gccgagcgcc cgacgtcgtg gcccgtgagg agcgcgccgt
     2581 cgcatcgtgg cgttcgagag actccgcgac ccgttccagc aggcgcggca gcgacgcacc
     2641 cgtggctcgt tgcagcgcga agcatcgtgc gagggcgggg gcagcggagc cgaggtactc
     2701 ctcggcccga tcgaacgcgg cggcgggaga ctcggccagc gcgacacacc gagagacgac
     2761 ggcgaccact ccgggagcgt cccagcgcgc gagcgcgacg tgcggagggg tcccgccgcg
     2821 gatcgacccg gcgagagagc ggatcgcccg cgcgagctcg tccggcggga gctctcggac
     2881 gcgctgccac gggagcttca tcgcgcctcg atctcttcca gcttgcgtct gccgtcccgc
     2941 cgggacaggt ggacgaacgc gtcgacggcc cggcgcacgc ggcccacgtc cgtctccgcg
     3001 agctcggcga ggcgttccgg tgcctcggcg gccgaacgcg cgtgcagcgt cagcatcgag
     3061 ccctcgtgac cggtcgccat cgccgccaga gccacggccg cctcgcgccc ccgcacctcg
     3121 ccgacgacga tgcggtcggg acgcatgcga agagaagcgc gcacgagcgc gtcgagcccg
     3181 accgcgccgg agccttcgac gttcgggggc cgcgctacca acgacacgac gtgcgacgag
     3241 aaggggccca gctccggcgt ctcctcgatc gtgacgacgc gttcggcagt gccgacgaga
     3301 gcgaggagcg cgttcagcag cgtcgtcttc ccggcccccg tagcgcccga gaccacgacc
     3361 gtcgcccgcg cggcgacgag atcgcgcagc cgggcggcgt tggcggccga cagcatctcc
     3421 gcgcggacga ggtcgtccag cgtgaagcgc accgacggga acctgcggat cgagacgagg
     3481 gggccgcgcg gggcgatcgg cgggaggacg acgtggatcc tcgacccgtc ggggagccgc
     3541 ccgtcggcga ccggcctcga cgcgtcgacc gagccgccgc cgcgtgcgag caggcggtcg
     3601 acgaagtccc ggagcgcgtc ggcgtcgggc cactcgatcg cctccctcgt cagtgtcccg
     3661 tcctgctcgg cccacacctc gcgcggcccg ttgacgagca cgtccgtcac cccgggccgc
     3721 gccatgacct cggcgagcgg cccgtacccg tcgacgtgct cggcgaccgc ggcgatcttg
     3781 tccggagcca cgacctcgcg cagggccagc cgtcgctcgg ccggatcgag cgtcgcgagc
     3841 cagtcgttgt cgaggacgcg gtcgagtagc gagcgactca gcacgacgac agcgccttgg
     3901 ccaaccgcgc tacgccgcgc gtccaccggc gccacgagcc cgccagcagc cgggcctcgt
     3961 cctcggcgtc gcgcagagcc gggcagcacg ggagctcgat cccgatcggg cggccgagca
     4021 gctgctcgag gcccccgcgc atgatctgtc ccccggggcc gagccggttc acgacgacga
     4081 tccaccgggc ctccgggaag gcctccgtca gagagcgcgc ggccagcgcg gcgggacgcg
     4141 tcggcgtcgt caccaacacg gccgcgcttg aagaagtcag gaccgcgtcg aggtcgcggc
     4201 gcgagcccag gtcgaggacc agtcgtccga acgacgcacg cgcctcgcgc agcgggaacg
     4261 cctccggcga cgggcaagga tccggtgcgc gcaggacgcg caaccctccg gcgaccggaa
     4321 ggccctgcaa cgaacggtcg tcgtcgcgcg gaagccacgt aagcgcgtcg ccgggaagac
     4381 ccagtcgcga cgaagccccc gccagctccg cgtagcccga cggcgccatc gccgcagcca
     4441 gatgaaccgc cagggaagtg accccgacgc cgccggcagc cccgactacg gcgtacacac
     4501 gtccccgtcc cacgcgcgcc tcgaccgcac gcagagcttc gcccggcctc tcccggtcga
     4561 acacgatccc gtccccggcc cccacgtcgc tcccgtacac gaccgcgtcg gcgtcgtcgg
     4621 gctcgtccga gcgcagctcc accgtccatt ccggcggcgc cgtcgcgaac gcagtcgcgg
     4681 ccgcatcccg aaccgcgggg tcgttgctca cgatcgcgac cttcgtcatg gacacagacg
     4741 ctcgcggatg acgagacggc cccctgtggc gcacgtcaca catggagggg acaatcgacg
     4801 acgtggaagc cgccttcttc gacctcgaca agacgatcat cgccaagtcc agccctctcg
     4861 cgctcgggcg gtcgttcttc cgcgagggct tgatcggacg gtcgttcctg ctgaagtccc
     4921 tctacgcgca gctcgtgttc gcgctgatgg gcgcggacga ggccaagatg gatcgcatgc
     4981 gcgtcgaggc cgcgaagctg acggcgggct gggagcagga gaaggtccgg caggtcgtcg
     5041 ccgaggtcct cgaggaggtc atctcgccgc tcatctacgc cgaggcgctc gagctcatcc
     5101 acgaccaccg cgccgcgggc cggctcgtgt gcatcgtgtc gtcgtcgccg gaggagatcg
     5161 tcgagcctct cgcgaacatg ctgcgcgcga accgcttcat cgccacgaaa ccgacgatcg
     5221 tcgacggcaa gtacaccggc gacctcgact tctacgcgta cggcccccac aaggccgcgg
     5281 cgatcgagga gctggcggac gagatgggca tcgacctgtc cggctcgttc gcctactcag
     5341 actcggtcac ggacctgccg atgttggagg tggtgggcaa ccccgtcgcg gtcaacccgg
     5401 acaaggcgct gcggaagatc gccctcgaga agggctggga cgtcgaggcg ttccgcaacc
     5461 cggtcccgct ccgcagcagg ctcccgcagc tgcggccgcc ggaggtctcg gtcccgggga
     5521 ggttcgtcgc cgctgcggga gtggcaggtg tggctgcggt cgcctggtgg tggacgagga
     5581 gaagcggatc caaggccgaa atagggtgag ctcgtgggac gaatgagacg ggttacgtgg
     5641 gcggcgctgc tcgcggtggc gttgttcgcc gggctgctgt cgcagtcggg cagcctcgcc
     5701 gcgcccaccg aagccgagct cgacgccgct cgggagcgcc tgatggagct cgaggccgag
     5761 ttcgagaccg tcgtcgagcg ctacaacctc gtccacgaga acctcacgtc gatccaggcc
     5821 cggatcggcg tcaccgagct cgaggtccgc cggatcgagc gccgcatggg cgtacggcgg
     5881 gaggccgccg tagacgtcgc ccgcgagctc tacatgggcg gctcgaacga cgcgctcgag
     5941 gcggtgctga tcgccgacga catcggcgac gtcgagtccc gcctcgccta cctgcgtacg
     6001 tcgcagcagg cgcagtccga ga
//
LOCUS       DASQDO010000274         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_640904, whole genome shotgun sequence.
ACCESSION   DASQDO010000274 DASQDO010000000
VERSION     DASQDO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640904"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEV43_07685"
     CDS             <1..261
                     /locus_tag="VEV43_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HYP23437.1"
                     /translation="PARLILKDGGTFEGTTGCRSFTGGWAVAGDVVEVTTMVFEGDCK
                     TAAEQDSHVASVLGGGFRAERKADRLTLTSEKLDLGLVYRSR"
     gene            272..901
                     /locus_tag="VEV43_07690"
     CDS             272..901
                     /locus_tag="VEV43_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HYP23438.1"
                     /translation="MLIRRADKPHSDEEWRSFLRTHDFGQFIAPGAGRPLPVVVPTHF
                     VYEGGDLALFHLARPNPVWRALEESPRGMLTVVGAYTYVTAELNTGPEDEPGYGVPTS
                     YYAAVQIGGRAEIVDDPDEMAELLNAQMQHFEPGREGHCVEASNEYGKLLPAIRGVRF
                     HFEDVKAKFKFGGNRKPEHARAIAEALSARGRDLDVEASAIQTRRLDER"
     gene            complement(903..1424)
                     /locus_tag="VEV43_07695"
     CDS             complement(903..1424)
                     /locus_tag="VEV43_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23439.1"
                     /translation="MRWRAIQPAIFGLAALSFMLPFAHVSCSTDQPAQSGPLVEDFGR
                     GSEELKGYELVVGRDLQREVAAGFAIEEGDVHVGGEPFAVVAFGAAVAGVGAVFVPQA
                     RRRAVTGLVAGAIGVLSLIVLGLAPVQRTFGLLEVSWKLGYWVCLTLFALATTVAYLE
                     QRDARPPPYLRRE"
     gene            1484..2914
                     /locus_tag="VEV43_07700"
     CDS             1484..2914
                     /locus_tag="VEV43_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYP23440.1"
                     /translation="MDTESTATTTEFDSPGGSPSRSVAGALDAAHELALRYVESLPDR
                     HVGGTASRDALLDALGGLLPEGPDDPAGVVRRLADAADPGLVATSSGRFFGFVEGGVL
                     PAALAADWLTSVWDQNPGFSVLSPAAAAAEEVVAGWMLDLLGLPPSASVGFTTGAQMA
                     SLAGLAAARHHVLAAVGWDAEADGLIGAPRVKVVVGDERHATIGRALRFLGFGERTVT
                     VVPADGQGRMDAGALGAELARDDGPAIVCAQAGNVNTGAFDPLRAIGEATREHGAWLH
                     VDGAFGLWAAASPRLRHLADGIELADSWATDGHKWLNVPYDSGIVVCAHPDAHRTALA
                     LHAPYLIRNAGNPRDGSDWTAESSRRARVFALWAALRSLGRTGVAGLVERCCDHARRF
                     ADELGGKPGFEVLNEVVLNQVLVRVGDDDQRTRAVAHAVQREGVAWLGETTRHGVVAL
                     RISVSDHATTEADVDRAIDAIVRAAS"
     gene            2926..3618
                     /locus_tag="VEV43_07705"
     CDS             2926..3618
                     /locus_tag="VEV43_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HYP23441.1"
                     /translation="MSDLRALYVNCTLKRSPEPSHTQLLVDRSVAIMRNQGVEVDQIR
                     AVDHDVATGVWPDMTEHGWETDEWPTLFERVMAADILVIASPIWLGEKSSVCTHLIER
                     LYGNSSLLNEAGQYAYYGRTGGCLITGNEDGIKHCSMSVLYALQHLGYVIPPQADAGW
                     IGEAGPGPSYGDDGAGLDNDFTNRNTTFMTWNLMHTARMLKDAGGFPARGNQRSEWDA
                     GCRWDQPNPEHR"
     gene            3618..4121
                     /locus_tag="VEV43_07710"
     CDS             3618..4121
                     /locus_tag="VEV43_07710"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYP23442.1"
                     /translation="MTLRGERVELRPLEPGDLDAVRRIRASDAVVRWWGRPDDDFPPD
                     DGDPRFAVVIGGAVAGMVQYYEEPDPEARHADVDVFLDAGYHGRGYGTDAMRTIVRHL
                     AEERGHHRVTLSTDPGNAAAIRCYEKVGFRAVGITQASRWDDVAEQWVDELLMDLVTL
                     PGAPPSS"
     gene            4203..>5188
                     /locus_tag="VEV43_07715"
     CDS             4203..>5188
                     /locus_tag="VEV43_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23443.1"
                     /translation="MGFRRSPEGQRDGESPQPLAEELAGQYRARILLDGTHAGAVASV
                     DGPALERAGLRKLAWMAKAAVVAKIASDLPFGEIVSTSVVGRDGDLTLVLEEGVAAAV
                     LHEWATSPRGRRREVVVELYDDEGRHSSTLKLKRARVAEHRLEEDPARPGAFRVDRLV
                     LAPREAEWFVAAIEPLAEIDDLFAQPAAPAAPPAACRGAFGRGMAAGLAAALLLGAMV
                     LVPGPRRGFDPAGAESPLPAGCPPPPGVVPGVACVEPTMLVTSRPSFAPVPPTPTPPP
                     PPVSPSPVPPPPPPVSPSPVIVSPPPVSPSPVIVSPPPVSPSPVMVSPPPVSP"
BASE COUNT          736 a         1848 c         1914 g          690 t
ORIGIN      
        1 ccggcgcgcc tgatcctgaa ggacggcggg acgttcgagg gcacgacggg ctgccgctcg
       61 ttcaccggag gatgggccgt cgcgggcgac gtcgtcgagg tgacgacgat ggtcttcgag
      121 ggcgactgca agacggcggc cgagcaggac tcgcacgtcg cctcggtgct cggcggaggc
      181 ttccgcgccg agcgcaaggc agaccgtctc acgctgacct ccgagaagct cgacctcggc
      241 ctcgtctacc ggtcgcgtta gcctcgtccg gatgctgatc cggcgcgcgg acaagccgca
      301 ctccgacgaa gagtggaggt cgttcctccg cacgcacgac ttcggccagt tcatcgcgcc
      361 gggcgccggg cgtccgcttc ccgtcgtcgt ccccacccac ttcgtctacg agggcggcga
      421 cctcgcgctg ttccacctcg cgcgtccgaa cccggtgtgg cgggcgctcg aggagagccc
      481 gcggggcatg ctcacggtcg tcggcgcgta cacgtacgtc acggccgagc tgaacaccgg
      541 gcccgaggac gagcccggct acggcgtccc cacgagctac tacgcggcgg ttcagatcgg
      601 gggccgcgcg gagatcgtcg acgacccgga cgagatggcg gagctcctga acgcgcagat
      661 gcagcacttc gagccgggcc gcgagggaca ctgtgtcgaa gcctcgaacg agtacgggaa
      721 gctgctgccg gcgatccgcg gcgtccgctt ccacttcgaa gacgtcaagg cgaagttcaa
      781 gttcggcggc aacaggaagc cggagcacgc acgcgcgata gccgaggcgt tgtcggcgcg
      841 gggccgcgac ctggacgtcg aggcgagcgc gatccagacg cggcgcctgg acgagcgctg
      901 agctactcgc gccggaggta gggcgggggc cgagcgtccc gctgctcgag gtaggcgacg
      961 gtggtggcga gcgcgaacag cgtcaggcag acccagtagc cgagcttcca ggagacctcc
     1021 agcaagccga aggtccgctg gaccggagcg agcccgagca cgatcagcga cagcaccccg
     1081 atcgcgcccg caacgaggcc tgtgacggcg cgccgccgag cctgcgggac gaagaccgcc
     1141 cccacgcccg cgactgccgc gccgaaggcc acgacggcga aaggctcgcc gccgacatgc
     1201 acgtcgcctt cctcgatcgc gaacccggcc gcgacttcgc gctggagatc acggcccacg
     1261 acgagctcgt accccttgag ctcctccgac ccgcgcccga agtcctcgac gaggggcccg
     1321 ctctgcgccg gctgatcggt cgagcaggag acgtgcgcga acgggagcat gaacgagagc
     1381 gcggcgagac cgaagatcgc cggttggatc gcccgccacc gcacccgtcg atcctaggcc
     1441 ccccgggtgg acgaacccgg agttcgcggc gactatcgcc ctcatggaca ccgaaagcac
     1501 cgccacgact accgagttcg acagccctgg cggatcgccg agccgatccg tcgccggggc
     1561 cctcgacgcg gcccacgagc tggcactgcg ctatgtcgag tcgctccccg accgccacgt
     1621 cggcggcacc gcgtcgcgcg acgcgctcct cgacgcgctc ggcggattgt tgcccgaagg
     1681 cccggacgat cccgcggggg tcgtgcgccg gctcgcggac gcggccgacc ccgggctcgt
     1741 cgcgacgagc agcgggcgct tcttcggctt cgtcgaggga ggcgtgctgc ccgcggccct
     1801 cgcggccgac tggctcacgt cggtgtggga ccagaacccg ggcttcagcg tcctgtcccc
     1861 cgcggccgcc gccgcggagg aggtcgtcgc gggctggatg ctggacctgc tcgggctccc
     1921 gccatcggct tcggtcgggt tcacgacggg tgcacagatg gcaagcctcg ccggcctcgc
     1981 ggcggcgcgc catcacgtgc tcgcggccgt gggctgggat gccgaggccg acgggctgat
     2041 cggcgcgccg cgcgtgaagg tcgtcgtggg cgacgaacgg cacgcgacga tcggacgggc
     2101 gctgcgcttc ctcggcttcg gcgagcggac ggtcacggtc gttcccgcgg acgggcaggg
     2161 ccggatggac gccggcgcgc tgggggccga gctcgcccgc gacgacgggc cggcgatcgt
     2221 gtgcgctcag gccgggaacg tgaacaccgg cgctttcgac cccctgcgcg cgatcggaga
     2281 ggcgacgcgt gagcacgggg cgtggcttca cgtcgacggc gccttcgggc tgtgggccgc
     2341 ggcgagcccg cggcttcgtc acctcgccga cgggatcgag cttgcggact cgtgggcgac
     2401 cgacgggcac aagtggctca acgtcccgta cgactcgggc atcgtcgtct gcgcccatcc
     2461 cgacgcgcac cgcacggccc tcgcgctgca cgcgccctat ctcatccgga acgccggcaa
     2521 cccccgtgac ggctcggact ggaccgccga gagcagccgc agggcgcgcg tgttcgcgct
     2581 gtgggctgcg ctccgcagcc tcgggcgcac cggcgtggcc gggctcgtgg agcgttgctg
     2641 cgaccatgcc cggcgcttcg ccgacgagct gggtgggaag cccgggttcg aggtgctgaa
     2701 cgaggtcgtg ctgaaccagg tcctcgtccg agtgggcgac gacgaccaaa ggacgcgtgc
     2761 ggtagctcac gcggtgcagc gcgaaggggt cgcgtggctc ggcgagacga cgcggcacgg
     2821 cgtcgtggcg ctgcggatca gcgtctcgga ccacgcgacg accgaggccg acgtcgaccg
     2881 cgcgatcgac gcgatcgtga gggcggcgtc gtgaccgaac tcgacatgag cgacctccgc
     2941 gcgctctacg tgaactgcac gctgaagcgc tcgccggagc cgtcgcacac gcagctcctc
     3001 gtcgatcgtt ccgtcgcgat catgcgcaac cagggggtcg aggtcgatca gatacgcgcg
     3061 gtcgaccacg acgtcgctac cggggtgtgg cccgacatga ccgagcacgg ctgggagacc
     3121 gacgagtggc cgaccctgtt cgagcgggtg atggcggccg acatcctcgt gatcgcgtcg
     3181 cccatctggc tcggcgagaa gtcgtcggtg tgcacgcact tgatcgagcg tctctacggg
     3241 aactcgagcc tgttgaacga agccggccag tacgcctact acggccgcac gggaggctgt
     3301 ctcatcaccg gcaacgagga cggcatcaag cactgctcga tgagcgtcct gtacgcgttg
     3361 cagcacctcg gctacgtgat cccgccacag gcagacgcgg gatggatcgg cgaggccggg
     3421 cccggcccct cctacggcga cgacggtgcg gggctcgaca acgacttcac gaaccgcaac
     3481 acgacgttca tgacctggaa cctcatgcac acggcgcgga tgctcaagga cgcgggcggc
     3541 ttccccgcgc gcggcaacca gagatcggag tgggacgcgg gctgccggtg ggaccagccc
     3601 aacccggagc accggtagtg accctgcgcg gcgagcgcgt cgagctgcgg ccgctcgagc
     3661 ccggcgacct cgacgcggtg cggcgcatcc gcgcgtccga cgccgtcgtg cggtggtggg
     3721 gacgccccga cgacgacttc ccgcccgacg acggcgaccc gcgcttcgcc gtcgtgatcg
     3781 gcggcgcggt cgcggggatg gtccagtact acgaggagcc cgaccccgag gcccgtcacg
     3841 ccgacgtcga cgtgttcctg gacgcggggt accacggccg gggctacggg accgatgcga
     3901 tgcggacgat cgtgcggcac ctggccgaag agcgcggcca ccatcgtgtg acgctgtcga
     3961 ccgaccccgg caacgcggcg gcgatccgct gctacgagaa ggtcgggttc cgcgccgtcg
     4021 ggatcacgca ggcgagcagg tgggacgacg tcgcggagca gtgggtcgac gagctgctga
     4081 tggacctcgt cacgcttccg ggggcgccac cctcctcctg accgggaacc cccttgcggg
     4141 agctctcgcg cgcggagatg cttttaccgg tcggaccgcg tgagttaagt gggaggcgcg
     4201 gagtggggtt ccgcaggtca ccggaaggcc agcgcgacgg ggagtcgccg cagccgctcg
     4261 cggaggagct ggccggccag taccgcgccc ggatcctctt ggacggcacg cacgccggcg
     4321 ccgtcgcctc cgtcgacggt cccgccctcg agcgcgcggg gctgcgcaag ctcgcgtgga
     4381 tggccaaggc cgcggtcgtg gcgaagatcg cttcggacct cccgttcggc gagatcgtgt
     4441 cgacgagcgt cgtcgggcgc gacggagacc tgaccctggt cctggaggaa ggcgtcgccg
     4501 cggcggtgct ccacgagtgg gcgacctctc ctcggggacg acggcgcgag gtcgtcgtcg
     4561 agctctacga cgacgagggc cgccactcga gcacgctgaa gctgaagagg gcgcgggtcg
     4621 ccgagcaccg cctcgaggag gacccggccc gccccggcgc cttccgcgtg gaccggctcg
     4681 tcctcgcgcc gagggaggcc gagtggttcg tggcggccat cgagccgctg gccgagatcg
     4741 acgacctgtt cgcgcagccg gcggctccgg ccgcgccccc ggctgcctgc cggggtgcgt
     4801 tcggccgcgg gatggcggcc gggctcgcag cggcgctcct gctgggggcg atggtcctgg
     4861 taccggggcc gcgacgaggg ttcgaccccg ccggggccga gtcgccgctg ccggcgggat
     4921 gcccgccgcc gccgggtgtg gtcccggggg tcgcctgcgt agagccgacg atgctggtga
     4981 cgtcgaggcc gtccttcgcc ccggtcccgc cgaccccgac tccaccgccg cctccggtga
     5041 gcccttcgcc ggtcccgccg ccgcctcctc cggtgagccc ctcgccggtg atcgtctcgc
     5101 cgcctccggt gtcgccctcg ccggtgatcg tctcgccgcc gccggtgagc ccgtcgcctg
     5161 tgatggtctc gccgccgccg gtgagccc
//
LOCUS       DASQDO010000275         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_151679, whole genome shotgun sequence.
ACCESSION   DASQDO010000275 DASQDO010000000
VERSION     DASQDO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151679"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VEV43_07720"
     CDS             <1..1179
                     /locus_tag="VEV43_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYP23444.1"
                     /translation="GGNFEDPLDRPLTAALLDEAVGDRAALLARADMHSCVVSSALLD
                     KLDVDRPSGYLREQDAAEAWKWFDSSLPRAQQRDAIRAAVDLAYSKGVTSVHEMYVVE
                     WRGWDSLEVMQEALEGAALEVVLYVATDEVERVREMGLPRIGGDYFLDGSFGSHTAWL
                     SHPYDTDPPEGSPPEGISYRDDGELLELFRAAQASGLQMGVHAIGDAAIEQAISTWEK
                     VAAEVGDEPVRSLGHRIEHFECAADDHIDRAARLGLRASVQPAFDHYWGGTDGLYADR
                     IGEERALQMNRFGSMVRKGLLLGAGSDSTVTPLDPFLQMGALRGHHVEAERLDPATAL
                     EMHTVGSRALAGDSSAGVIAAGRRADLVLVDRDPLAAEDVTQTQVLRTWIAGEKVWPA
                     "
     gene            1176..>2324
                     /locus_tag="VEV43_07725"
     CDS             1176..>2324
                     /locus_tag="VEV43_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HYP23445.1"
                     /translation="MRPFTRPVGRLGIDHETVAECRSLAAEITAPVEDLARSHTTVSI
                     ERACLRLCGVDGVLGEGTEAVPIPNAVVDAIRLSAGLQRGAMLPFFGAVASSGLGVQE
                     TAQAIANGELEVPLTDEGRGLAEDAARKAVDRIEAARTSRAEHIARAGEAEKPWMYLI
                     VATGNIHEDVPQAQAAARNGADVIAVIRSTGQSLLDYVPYGATTDGFAGTFATQENFR
                     LMRAALDEVGEELGRYVRLTNYASGLCMPEIAAMAAMERLDMMLNDCMYGIIFRDINM
                     QRTFVDQHFSRMVHALAGIVINTGEDNYLTTADAVDAAHTVLASQFLNERLARVAGLD
                     EEQMGLGHAFEIDPAVPDQIMMELAHAQLVRECFPRHPLKYMPPTKHMT"
BASE COUNT          360 a          774 c          854 g          336 t
ORIGIN      
        1 ggaggcaact tcgaggatcc gctggaccgg cccttgaccg cggcgctcct cgacgaagcc
       61 gtgggggacc gcgctgcgct cctggcccgg gcggacatgc actcgtgcgt ggtgtcgagc
      121 gcgctcctgg acaagctcga cgtcgaccgg ccctcgggtt acctgcgcga gcaggacgcc
      181 gcggaagcgt ggaagtggtt cgactcttcc ctgccgcggg cccagcagcg cgacgcgatc
      241 cgcgccgccg tcgacctcgc gtactcgaag ggcgtcacgt ctgtccacga gatgtacgtc
      301 gtggagtggc gcggctggga ctcgctcgag gtcatgcagg aggcgctcga gggcgcggcc
      361 ctcgaggtcg tgctgtacgt cgcgaccgac gaggtcgagc gcgtgcggga gatggggctg
      421 cctcggatcg gcggcgacta cttcctggac gggtcgttcg gctctcacac cgcgtggctg
      481 tcgcacccgt acgacacgga ccctccggaa gggtcgccgc ccgagggcat ctcctaccgc
      541 gacgacggcg agctgctcga gctgttccgg gctgcccagg cgtcgggcct ccagatgggc
      601 gtgcacgcga tcggtgacgc cgcgatcgag caggcgatct cgacgtggga gaaggtcgcg
      661 gccgaggtgg gcgacgagcc cgtgcggtcc ctcggccacc gcatcgaaca cttcgagtgc
      721 gcggcggacg accacatcga ccgcgctgcc cggctgggct tgcgagcgag cgtccagccc
      781 gcgttcgacc actactgggg cgggaccgac gggctgtacg cggacaggat cggcgaggag
      841 cgggcgttgc agatgaaccg cttcggctcg atggtccgca agggcctgct gctcggcgcg
      901 ggatcggact cgacggtaac gccgctcgac ccgttcctgc agatgggagc cctgaggggg
      961 catcacgtgg aagccgagcg actcgacccc gcgaccgcgc tggagatgca caccgtcggc
     1021 tcgcgcgctc tcgctggtga ctcgtcggcg ggggtcattg ccgccggacg gcgtgccgac
     1081 ctcgtcctcg tcgacaggga ccctctggct gcggaggacg tgacgcagac gcaggtcctc
     1141 cgcacgtgga tcgccggcga gaaggtgtgg ccggcatgag gccgttcacg cgcccggtgg
     1201 gccggctcgg gatcgaccac gagaccgtgg cggagtgccg ctcgctcgcg gccgagatca
     1261 ccgcgcccgt agaagacctc gcccgttccc acacgaccgt gtcgatcgaa cgggcgtgtc
     1321 tgcggttgtg cggggtcgac ggggtgctgg gcgaggggac cgaggcggta cccatcccga
     1381 acgcggtcgt cgacgcgatc cggctcagcg ccggtctcca gcgcggggcc atgcttccgt
     1441 tcttcggtgc cgtggcgtcg tcggggctcg gagtccagga gacggctcag gcgatagcga
     1501 acggcgagct cgaggtcccg ttaacggacg agggccgggg gcttgccgag gacgcggccc
     1561 ggaaggcagt cgaccggatc gaggcggcga ggacttcgcg cgcggagcac atcgcgcgcg
     1621 caggtgaagc cgagaagccg tggatgtacc tcatcgtcgc gaccggcaac atccacgagg
     1681 acgtgccgca ggcgcaggcc gcggcccgca acggcgccga cgtcatcgcg gtcatccgct
     1741 cgaccgggca gagcctcctc gactacgtgc cctacggcgc gacgaccgac ggtttcgccg
     1801 ggacgttcgc gacgcaggag aacttccgcc tgatgcgggc cgcgctcgac gaggtcggcg
     1861 aggagctcgg gcgctacgtc cggctcacga actacgcgag cgggctgtgc atgccggaga
     1921 tcgcggcgat ggccgcgatg gagcggctcg acatgatgct caacgactgc atgtacggga
     1981 tcatcttccg ggacatcaac atgcagcgga cgttcgtcga ccagcacttc tcgcgcatgg
     2041 tccacgcgct cgccggcatc gtgatcaaca cgggtgagga caactacctg acgacggcag
     2101 acgcggtgga cgcggcgcac acggtgctcg cgtcgcagtt cctcaacgag cggctggcgc
     2161 gcgtagcggg tctggacgaa gagcagatgg gtctcgggca cgccttcgag atcgacccgg
     2221 cggtgcccga ccagatcatg atggagctcg cgcacgcaca gctcgtccga gagtgcttcc
     2281 cgcgtcatcc gctcaagtac atgccgccga ccaagcacat gacc
//
LOCUS       DASQDO010000276         8012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1191470, whole genome shotgun sequence.
ACCESSION   DASQDO010000276 DASQDO010000000
VERSION     DASQDO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191470"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..864)
                     /locus_tag="VEV43_07730"
     CDS             complement(7..864)
                     /locus_tag="VEV43_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP23446.1"
                     /translation="MATQAGRITRTFGVPGRLLNRGIDGLANIGAQLSFMAKSLWDII
                     PGLRHGGETARLISEITLGSGALIVGAGTAGVIFFMAFFTGTQVGLEGFKGLQLIGAE
                     AFTGFVSAFANTREITPLIAGVTLAAQVGAGFTAELGAMRVNEEIDALEVMAVRPVPY
                     LVSTRIIAALTAITPLYLISLFSSYIATELVVTKLLGLSGGTYAHYFSLFLPPTDVLY
                     SLIKAAVFAITVALIHCYYGFNASGGPAGVGIAVGKAIRASIVAVNILNLMMSSFMWG
                     VEETVRIAG"
     gene            complement(872..1645)
                     /locus_tag="VEV43_07735"
     CDS             complement(872..1645)
                     /locus_tag="VEV43_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP23447.1"
                     /translation="MAQPMRTLVVETGNMFAITLEAFRIMWKPPYPWREFVRQSWFIA
                     SVSMIPAVLVSIAFGATIALQVGNVARQLGAESQTGAAMVLAVVREAAPIVAALLIAG
                     AGGSAMCADIGSRKIRDEISAMEVLAVNPIATLVVPRIWAAIFVAMLLDGFVSVAGIL
                     GGYFFNVIIQGGTPGAYLASFSLLAQTADLIVAILKAGVFGLVAAMVAVYFGFYAKGG
                     PKGVGDAVNRAVVVTFMLIFFVNFILTALYFAIVPQKFG"
     gene            complement(1648..2541)
                     /locus_tag="VEV43_07740"
     CDS             complement(1648..2541)
                     /locus_tag="VEV43_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP23448.1"
                     /translation="MLETEISIKNLTKSFGPQTVLEDITLDIPKGKITLMLGPSGTGK
                     SVFLKHLVGLLKPDRGEIWIDGKNVPALNEKELFEVRRKFGVLFQDGALFGSMNIFDN
                     VAFPLRQHTRKKESEIKQIVDEKLHMVGLTGAEKKLPGEISGGMKKRAGLARALVLDP
                     EIVLFDEPDSGLDPVRTAFLNELTMDLNRQLKATFIVVTHDIATARKVSDFIGMLYRR
                     NLVRFGPAREMFESEIPVVRQFLSGDTEGPIGMSEEKDSTKAVTSSSGMTADETEEME
                     RSAGAPPADAQEPATTRRKSF"
     gene            2698..3027
                     /locus_tag="VEV43_07745"
     CDS             2698..3027
                     /locus_tag="VEV43_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23449.1"
                     /translation="MDFRDTPEEAAFRSELRAWIEDNIPEGWSSRGPSSGRGEEETSR
                     EWARRLFEGGYAGLTWPKEYGGAGAPYTHQAIFLEEIARAEAPEHIGVIGIGMAGPTI
                     IVHGTDE"
     gene            3067..3879
                     /locus_tag="VEV43_07750"
     CDS             3067..3879
                     /locus_tag="VEV43_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23450.1"
                     /translation="MWCQGFSEPGSGSDLASLRTRAERDGDDFVVNGQKVWSSFAHIA
                     QHCILMVRTDASAPKHQGITYLLADMDAPGVEVRPLRPITGDPEFNEIFFSDVRVPAA
                     NVLGEVNGGWGVAMTTLMHERGTLGFALTARLEVAVKNLVALAKETRTNGRLASDDPV
                     FRDRIARQWIELQELRFTNYRALTGLVKTGVPGPEGSIAKLHWSESNQRLTKLALEIL
                     GPRAPMEGAEGPWNGYWQYQQLRSRGNTIEAGTSEVLRNIISERVLGLPRSR"
     gene            3879..4982
                     /locus_tag="VEV43_07755"
     CDS             3879..4982
                     /locus_tag="VEV43_07755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23451.1"
                     /translation="MDFAFSEEQQMLRDAVASRAGDRLPEGKVAELATSDAGWDPGSL
                     AEMAELGWVTLSWPEEAGGSGMGFLEETVVIEELGKALYPGPYLSSVALAAPALRRSP
                     ELLGRIAAGEGATLAWAEPEGPWTFEDLESVGTKAAQNGGGWTLTGVKDLVPDLGACS
                     FAVVVARSPEGLGLWAVDVAGAGVEASVLPTVDATRRLGRLTLTDAPAQLVVAPDAAG
                     DVLRGVRLRTLAALAIEAVGVASTAVDLARSYVSERKQFEKPIGAYQAVSHQVTDSYM
                     AVELARSLAYWAAWCVDQHDPQAPVAVAAAKASAGEAAVFACERAIQVHGGIGFTWEH
                     VLHRYYKRAQWIATFEGAGSHHREIVAQRLLDA"
     gene            5023..6213
                     /locus_tag="VEV43_07760"
     CDS             5023..6213
                     /locus_tag="VEV43_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP23452.1"
                     /translation="MPGVRTASLIESLPGRLRDVPLPAGSRYRLRIGRTVRDVVFSRN
                     SCVVGPAEGSPHVEIVTDAATWAEIDAGRMSGIEAFAQRRLVVRGSIDKSLLFEPLFD
                     RPSGGGLEYEVDDVRVGRVTFSRLVAGPEEAPPLLLLHGLGASKASWLTVVPRLARHH
                     RVIAIDLPGFGSTSKPRGRYDAPWFAKHVLSFMEVTGLHDTLVAGNSMGGRVAMEVAM
                     REPRMVRAIACLCPVAAFYERPFLWAARMARPEAGVFAARLPRAYLAEQLRRLFSDPT
                     RLHDDWYEAAIDDFLQTWRRPTARMAFLASLKNIYVEEPSGDAGFWARLAKLRVPALY
                     VYGRQDVLISSRFGGRVAKALPKAKVEIWDDCGHAPQLEHPDRTAETMLDFFAEAKPT
                     RRAV"
     gene            6250..6549
                     /locus_tag="VEV43_07765"
     CDS             6250..6549
                     /locus_tag="VEV43_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP23453.1"
                     /translation="MSTWAGDLIKIARLEAKLTQRELARRAGTSQAALSSYESGKKSP
                     TLETLHRIVLAAGQDLRIQVVPYDPHDDWVAAYEASLDPRELERWWEIAGSAKPA"
     gene            6546..7040
                     /locus_tag="VEV43_07770"
     CDS             6546..7040
                     /locus_tag="VEV43_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23454.1"
                     /translation="MTRLDPPSMFRILAVHDVEYVLIGAMAAVVQGAGIPATADVDIA
                     PAGDEENRARLAAALREMGARLRLAGDEDPVPIKLDARTFKGISVMTFETEHGPFDVL
                     FEPSGAPPYAELYARGATMSLSGVDVRVAAVEDVIAMKRAAGRRKDAPHIVKLLEYLQ
                     KRSS"
     gene            complement(7037..7279)
                     /locus_tag="VEV43_07775"
     CDS             complement(7037..7279)
                     /locus_tag="VEV43_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23455.1"
                     /translation="MRRLVTPAALAAVVSFGAAAPASADPLGCTEQAGENFQYGPGTS
                     PVVTIDGIDVTIHGSNASYFVRATGGVALQFAECVV"
     gene            complement(7344..>8012)
                     /locus_tag="VEV43_07780"
     CDS             complement(7344..>8012)
                     /locus_tag="VEV43_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23456.1"
                     /translation="ITNAPIADFMIVVAYTDPDAGIEGMALFVVDLPATGVAINRLSK
                     MGNLSSEIAEVVLDDVVVPADACLGGEWGAFKKVMQTLNVGRVLVSGGAIGVARAALE
                     GAVAYANDRHAFGRPIGSYQGVSFPLARSHATIEAARLATYKAAWLFDEGRNPVLETS
                     TAKLLAAEAALFVTDAAIRVFGGYGYMREYPIERYYRDARYFAIVEGTGEIHQRVIAR
                     ELGL"
BASE COUNT         1269 a         2717 c         2786 g         1240 t
ORIGIN      
        1 gccctgctat ccggcgattc ggaccgtctc ctccacgccc cacatgaacg acgacatcat
       61 caggttgagg atgttcacgg cgacgatcga cgccctgatg gccttgccga ccgcgatccc
      121 cacacccgcc ggcccgcccg acgcgttgaa cccgtagtag cagtggatga gcgcgaccgt
      181 gatcgcgaac accgccgctt ttatcaacga atagaggacg tcggtcggcg ggaggaacag
      241 cgagaagtag tgcgcgtacg tcccgcccga caggcccagc agcttcgtga cgacgagctc
      301 ggtcgcgatg tacgaggaga acagcgagat caggtacagg ggcgtgatcg cggtgagcgc
      361 cgcgatgatc ctggtcgaga cgaggtaggg gaccgggcgc acggccatga cctcgagcgc
      421 gtcgatctcc tcgttgacgc gcatcgcccc gagctcggcg gtgaacccgg cgccgacctg
      481 cgcggccagc gtgacgccgg cgatcagcgg cgtgatctcg cgggtgttcg cgaacgcgga
      541 gacgaagccg gtgaacgcct cggccccgat cagctggagt cccttgaagc cctcgagccc
      601 gacctgggtc cccgtgaaga acgccatgaa gaagatgacc ccggcggtcc cggccccgac
      661 gatgagcgcg cccgagccga gcgtgatctc cgagatcagc cgcgcggtct cgccgccgtg
      721 acgcaggccc gggatgatgt cccacagcga cttcgccatg aacgacagct gcgcgccgat
      781 gttcgcgagg ccgtcgatgc cgcggttgag gaggcgtccg gggaccccga acgtcctcgt
      841 tatgcggccg gcctgagttg ccatctagct cctatccgaa cttctgcggg acgatcgcga
      901 agtacagcgc cgtcaggatg aagttgacga agaagatcag catgaacgtc acgacgacgg
      961 cgcggttcac cgcgtcgccc acgcccttcg gcccgccctt cgcgtagaac ccgaagtaga
     1021 cggcgaccat ggccgcgacg aggccgaaca ccccggcttt caagatcgcg actatgaggt
     1081 ccgccgtttg cgcgagtaac gagaacgagg cgagatacgc accaggcgta cctccttgga
     1141 tgatcacgtt gaagaagtag ccgccgagga tcccggcgac cgagacgaat ccgtcgagca
     1201 gcatcgccac gaagatcgcg gcccagatgc ggggcacgac cagcgtcgcg atcgggttga
     1261 ccgccagcac ctccatcgcc gagatctcgt cgcggatctt gcggctgccg atgtcggcgc
     1321 acatagccga cccgccggcg ccggcgatca ggagcgcggc gacgatcggc gcggcctcgc
     1381 gcacgacggc gaggaccatc gcggcccccg tctgcgactc ggcccccagc tgccgggcga
     1441 cgttgccgac ctggagggcg atcgtggcgc cgaacgcgat cgagacgagg acggccggga
     1501 tcatcgagac gctcgcgatg aaccacgact ggcggacgaa ctcgcgccac ggataggggg
     1561 gcttccacat gatccggaag gcctccagcg tgatggcgaa catgttcccc gtctcgacga
     1621 cgagggtccg catcggctga gccatggcta gaagctcttc ctccgcgtcg tcgcaggttc
     1681 ctgggcgtcg gccggcgggg cgcccgcgga gcgctccatc tcctcggtct cgtcggccgt
     1741 catcccggag ctgctcgtga cggccttcgt cgagtccttc tcctccgaca tgccgatggg
     1801 gccctcggtg tcgccggaga ggaactgcct gacgaccggg atctcggact cgaacatctc
     1861 gcgtgcgggg ccgaaacgca cgaggttgcg ccggtagagc atcccgatga agtcggagac
     1921 cttccgggcg gtcgcgatgt cgtgggtcac gacgatgaac gtggccttca gctgccggtt
     1981 caggtccatc gtcagctcgt tgaggaacgc ggtgcggacc gggtcgaggc cggagtccgg
     2041 ctcgtcgaac aggacgatct cggggtcgag cacgagggcc cgggccaggc ccgcacgctt
     2101 cttcatcccg ccggagatct cgccggggag cttcttctcg gcccccgtga ggccgaccat
     2161 gtggagcttc tcgtcgacga tctgcttgat ctccgactcc ttcttgcgcg tgtgctggcg
     2221 cagcgggaag gcgacgttgt cgaagatgtt catcgacccg aacagggccc cgtcctggaa
     2281 gagcacgccg aacttgcgcc ggacctcgaa gagctccttc tcgttgagcg ccgggacgtt
     2341 cttgccgtcg atccagatct cgccgcggtc gggcttgagg aggcccacga ggtgcttgag
     2401 gaagaccgat ttgcccgtcc cggagggccc gagcatcagc gtgatcttgc ccttcgggat
     2461 gtcgagggtg atgtcctcga ggaccgtctg ggggccgaac gacttcgtca gattcttgat
     2521 gctgatctcg gtctcgagca tgtcgtctcc acccgggtct gtgtgcgcgc gggcgaccga
     2581 ggtccccccg actggtcgtg ctttcaggta cttcggccgc caccctgcgg ccgggggaga
     2641 agcctactcc cgaatggcaa cttttctcac cggaatcggg gcgcctaaga tgccgccgtg
     2701 gatttccgcg acacccccga ggaggccgca ttccggtcgg agctgcgtgc ctggatcgag
     2761 gacaacatcc cagaaggatg gtcgagccgc ggcccctcga gcgggcgcgg cgaggaagag
     2821 acgtcgcgtg agtgggcgag acggctcttc gagggcgggt acgcgggcct gacctggccg
     2881 aaggagtacg gcggcgcagg ggctccctac acccaccagg cgatcttcct cgaggagatc
     2941 gcgcgggccg aggcccccga gcacatcggc gtgatcggga tcggcatggc cggcccgacg
     3001 atcatcgtcc acgggaccga cgagtagaag gcccgctacc tccccaagat cctgtcgggc
     3061 gaggaggtgt ggtgccaggg gttctccgag ccgggctcgg gctccgatct cgcgtcgctg
     3121 cggacgaggg ccgagcgtga cggcgacgac ttcgtcgtga acggccagaa ggtgtggtcg
     3181 tcgttcgccc acatcgccca gcactgcatc ctcatggtcc ggaccgacgc gtcggcgccg
     3241 aagcaccagg gcatcacgta cctgctcgcc gacatggacg cgcccggcgt cgaggtgcgg
     3301 ccgctgcgcc cgatcacggg cgaccccgag ttcaacgaga tcttcttcag cgacgtgagg
     3361 gtcccggccg cgaacgtgct cggcgaggtg aacgggggct ggggcgtcgc gatgacgacg
     3421 ctgatgcacg agcgggggac gctcggtttc gcgctcaccg ctcgtctgga ggtcgcggtc
     3481 aagaacctcg tcgcgctcgc gaaggagaca cgcacgaacg gccggctcgc gtcggacgac
     3541 cccgtgttcc gcgaccgcat agcgcgtcag tggatcgagc tccaggagct gcggttcacg
     3601 aactaccgcg cgctgaccgg cctcgtgaag accggcgtcc ccggaccgga gggcagcatc
     3661 gcgaagctcc actggtccga gtccaaccag aggctgacga agctcgcgct cgagatcctc
     3721 ggcccccgcg cgccgatgga gggcgcggag gggccgtgga acggctactg gcagtaccag
     3781 cagctgcgca gccgcggcaa cacgatcgag gccggcacgt ccgaggtgct gcgcaacatc
     3841 atctccgagc gcgtcctcgg cctcccgagg agccgctagt ggacttcgcc ttctccgaag
     3901 agcagcagat gctgcgcgac gcggtcgctt ccagggccgg cgaccgtctc cccgagggga
     3961 aggtcgccga gctcgcgacg agcgacgcgg gatgggatcc cggctcgctc gcggagatgg
     4021 ccgagctcgg atgggtgacg ctgtcgtggc ccgaggaagc cggcgggtcg ggcatggggt
     4081 tcctcgagga gacggtcgtg atcgaggagc tgggcaaggc gctctacccc gggccgtacc
     4141 tgtcgtcggt cgcgctcgca gcgcccgcgc tgaggcggag cccggagctc ctcgggcgca
     4201 tcgcggcggg ggagggggcg acgctcgcat gggccgagcc cgaagggccg tggacgttcg
     4261 aagatctcga gtcggtcggg accaaggccg cgcagaacgg tggcggatgg acgctgacgg
     4321 gggtgaagga cctcgtcccg gacctgggcg cgtgctcgtt cgccgtcgtc gtggctcgtt
     4381 cgccggaggg tctcgggttg tgggcggtcg acgtcgcggg ggccggggtc gaggcctcgg
     4441 tgcttcccac cgtcgacgcg acgcggagac tcggccggct cacgttgacg gacgcgcccg
     4501 cgcagctggt cgtcgctccc gacgcggcgg gagacgtcct tcgaggagtg cgtcttcgaa
     4561 cgctcgcggc cctggcgatc gaagcagtcg gagtcgcgag cacggccgtc gatcttgccc
     4621 gctcctacgt ctcggagcgc aagcagttcg agaagcccat cggcgcgtac caggcggtgt
     4681 cgcatcaggt gacggactcg tacatggcgg tcgagctcgc acgctcgctc gcttactggg
     4741 cggcctggtg cgtcgaccag cacgatcctc aggccccggt cgcggtggcc gcggcgaagg
     4801 cttcggcggg cgaggccgcg gtgttcgcat gcgagcgcgc gatccaggtc cacggcggca
     4861 tcggcttcac gtgggagcac gtgctgcacc gctactacaa gagggcgcag tggatcgcga
     4921 cgttcgaggg ggccggatcg caccaccgcg agatcgtggc gcagcgcctg ctcgacgcgt
     4981 gaatgcggcg cgaacattcc gcgttgctct aatgtcgcgg gcgtgccggg ggttcgtacc
     5041 gcttcgctga tcgagtcgct gcccgggcgc ctgcgcgacg tcccactccc ggcgggctct
     5101 cgctatcgcc tccgcatcgg ccgcaccgtg cgcgacgtcg tcttctcgcg taactcatgt
     5161 gtcgtcggtc ccgccgaggg ctccccgcac gtcgagatcg tcaccgacgc ggcgacgtgg
     5221 gccgagatcg acgccggccg catgtcgggc atcgaggctt tcgcgcagcg ccgcctcgtc
     5281 gtgcgcggca gcatcgacaa gtcgctgttg ttcgagccgt tgttcgaccg cccttcgggg
     5341 ggtggcctcg agtacgaggt cgacgacgtc cgcgtcggcc gcgtgacgtt ctcacggttg
     5401 gtcgcggggc ccgaggaggc gccccctttg ttgctactgc acggcctcgg cgcgtcgaag
     5461 gcgtcgtggc tgacggtcgt cccgcggctc gcgcgtcacc accgcgtgat cgcgatcgac
     5521 ctgccgggct tcggctcgac gtccaagccg cgcggccgct acgacgcgcc gtggttcgcg
     5581 aagcacgtgc tctccttcat ggaggtgacc ggcctgcacg acacgctggt cgccgggaac
     5641 tcgatgggag gccgcgtagc gatggaggtc gccatgcgcg agccgcgcat ggtccgcgcc
     5701 atcgcctgcc tctgccccgt ggccgccttc tacgagcggc cttttctgtg ggctgcccgc
     5761 atggcgagac cggaggccgg ggtgttcgcg gcccggctcc cgcgggcgta cctcgccgag
     5821 cagctccgcc ggctgttctc ggatccgacc aggctccacg acgactggta cgaggcggcc
     5881 atcgacgact tcctccagac gtggcgccgt ccgaccgccc ggatggcgtt cctggcgtcg
     5941 ctcaagaaca tctacgtgga ggagccgtcg ggggacgccg ggttctgggc ccggctggcg
     6001 aagctccggg tcccggccct gtacgtctac gggcgccagg acgtgctgat ctcgtcccgc
     6061 ttcgggggcc gggtggcgaa ggctctacct aaggcgaagg tagagatatg ggacgactgc
     6121 ggccacgcgc cccagctgga gcaccccgac cgcacggccg agacgatgct ggacttcttc
     6181 gcggaagcca agccgacccg ccgggcggtc taggcccctt tcggacaccc gatcggatat
     6241 catccggcca tgagcacgtg ggccggagac ctgatcaaga tcgcccgcct ggaggcgaag
     6301 ctgacgcaac gcgagctggc gcgccgcgcc gggacctcgc aagcggcgct gtcgtcctac
     6361 gagtcgggca agaagtcgcc gacgctggag acgctccacc ggatcgtcct ggcggcgggc
     6421 caggacctgc ggatccaagt cgtcccgtac gacccgcacg acgactgggt cgcggcgtac
     6481 gaagcgtcac tcgacccgcg cgagctcgaa cgttggtggg agatcgcggg ctctgccaag
     6541 ccggcgtgac gcgtctcgat ccgccgtcga tgttccgcat cctcgcggtg cacgatgtcg
     6601 agtacgtctt gatcggagcg atggccgcgg tcgttcaggg tgcgggtatc cccgctaccg
     6661 cggacgtcga catcgcgcct gccggcgacg aggagaatcg ggcccgcctg gccgccgcgc
     6721 tgcgcgagat gggcgcgcgg ctgcgactcg ccggagacga ggacccggtc ccgatcaagc
     6781 tcgacgcgcg gacgttcaag ggaatctccg tcatgacgtt cgagaccgag cacgggccgt
     6841 tcgacgtgtt gttcgagccg tcaggagctc cgccatacgc ggagctttac gcccggggtg
     6901 cgacgatgtc gctgtccggc gtcgacgtcc gcgtcgcggc cgtcgaggac gtcatcgcga
     6961 tgaaacgggc cgccggtcga cgaaaagacg ccccgcacat cgtgaagctg ctcgagtacc
     7021 tccagaaacg aagcagctag acgacgcact ccgcgaactg gagggccacc ccgccggtcg
     7081 cgcggacgaa gtagctcgcg ttcgaaccgt ggatcgtcac gtctatgccg tcgatcgtga
     7141 cgaccggcga cgtcccgggg ccgtactgga agttctcgcc cgcctgctcc gtgcagccga
     7201 gaggatcggc ggatgccggc gcagcggctc cgaacgagac gactgcggcc agcgcggcgg
     7261 gcgtgacgag acggcgcatg gtccctcctc ttcggcgtgg tcgtgacgaa atcgggcgaa
     7321 ccataccaag cagggcagcg cggctaaagg cccagctccc ttgcaatgac acgttggtgg
     7381 atctcgcccg tgccctcgac gatcgcgaag tagcgcgcat ccctgtagta gcgctctatc
     7441 gggtactccc gcatgtagcc gtagcccccg aagaccctga tcgccgcatc cgtcacgaac
     7501 agggccgcct cggccgccag cagcttcgcc gtcgacgtct ccaataccgg gttccggccc
     7561 tcgtcgaaca gccacgccgc tttgtacgtc gccagcctcg ccgcttcgat cgtcgcgtgc
     7621 gaacgcgcca gcgggaacga cacaccctgg tacgacccga tcggccggcc gaacgcgtgc
     7681 ctgtcgttcg cgtacgcgac cgcgccctcg agggccgccc gcgcgacccc aatcgcacct
     7741 ccggagacca acacacgccc gacgttcaac gtctgcatca ccttcttgaa cgcgccccac
     7801 tcgccgccca ggcacgcgtc ggcgggcacg acgacgtcgt ccagcacgac ctccgcgatc
     7861 tccgacgaga ggttgcccat cttcgacagc cggttgatcg caaccccagt ggccgggagg
     7921 tcgaccacga acaaggccat gccctcgatc cccgcatcgg gatcggtgta cgcgacgacg
     7981 atcatgaagt cggctatcgg cgcgttcgtg at
//
LOCUS       DASQDO010000277         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_28810, whole genome shotgun sequence.
ACCESSION   DASQDO010000277 DASQDO010000000
VERSION     DASQDO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28810"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VEV43_07785"
     CDS             complement(<1..1081)
                     /locus_tag="VEV43_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYP23457.1"
                     /translation="MPPSKTPTKSRKRSKMLILDGYSLAFRAFYALPEDLQTTDGTKT
                     NAVYGFTAMLIKLLQEQKPEYITVCFDVGEPLERSAEYAEYKSNRSTAPDGFSGQVPL
                     IYEVLRVMQIPIFGVPGHEADDVIACFAKRAAAEGIDVRIVTGDRDFFQLVSKDIRVL
                     YNRRGITDIVEMDPKKVEERYGVPPAKYVDLKALEGDTSDNLPGVPGVGTKTAAKLVQ
                     KYGSAEEAVAHASEQTPKLAANLAAHAEQVAINKRLSTLAEVPLAAEIEDLKMGPWDL
                     EEVRELFISLEFRTLLERLMADLPDAAEGEGEPFELEVRVVEDPAGVAELVADLRAAS
                     AGVAVDLVPSSPRGVPQSLAFSWDEG"
     gene            1107..1793
                     /locus_tag="VEV43_07790"
     CDS             1107..1793
                     /locus_tag="VEV43_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYP23458.1"
                     /translation="MSHSSPDLRALNPASKEYLEAVFELGEEGQRILQARIGERLGLA
                     PATVSEGIKRLVGEGYVTVDSSRQISLTDEGRTVAEALVRRHRLAERMLVDILGIPWH
                     LCHQQAEDWEKVMTPEVEQAILAKLDGEATCPHGNPIPGEPSSVPWSDLKSVASMTVG
                     ERGRLKRLLEDVELDGEVLEYLEQHRLMPGYELELVEVAPDGTRVLEVDGDRVALGTR
                     LADNLWVLVA"
     gene            1858..2433
                     /locus_tag="VEV43_07795"
     CDS             1858..2433
                     /locus_tag="VEV43_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23459.1"
                     /translation="MTLRIAAALIVALLAFSACDSDDGASVRDLGGSASGSGSGSGSG
                     TGAASGSGSATEAEAEACEPGDEADPALVVRLDEYSVVPVEEKLTSGPTRFRAVNAGE
                     EVHELYLAEGKSLEGLPLDPKGSVDTEALEEDGRLLGEVEGIPSGESCDLEADLEIGT
                     YVLFCNIRERTDEGVVNHFLQGMRARVKVDA"
     gene            complement(2417..>3117)
                     /locus_tag="VEV43_07800"
     CDS             complement(2417..>3117)
                     /locus_tag="VEV43_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23460.1"
                     /translation="RRGERRDVAWLGALLTLSGMLFVAAHWAWYRGWTPYAVGDHFVT
                     GELGVVGFDPNLAGRSTRLIGLLVDREFGLAAWQPAWLFAVPAAAALAARRPLGWAFL
                     LVPAAAGWLNATFVALTMHGWWFPGRQVVVVAPLLVIAVAAWAARSRPRLLGAAGLGA
                     AGVVSWAWLAQEALARRLTLVVDFYETSNPLYRAWSWLLPEYRAPTGATWVLHALWIV
                     AALAALWWGATRRP"
BASE COUNT          460 a         1091 c         1127 g          439 t
ORIGIN      
        1 ggccctcgtc ccacgagaac gcaagcgact gggggacgcc ccggggagat gacggcacga
       61 ggtcgaccgc gaccccggcc gaggcggccc ggagatcggc gaccagctcg gcgacgccgg
      121 cggggtcctc gacgacgcgg acctccagct cgaagggctc gccctcgccc tcggcagcgt
      181 cgggcaggtc ggccatcagg cgctcgagca gcgtgcggaa ctcgagcgag atgaacagct
      241 cgcggacctc ctcgaggtcc cacggcccca tcttcaggtc ttcgatctcg gcggccagag
      301 gcacctcggc gagcgtcgac agccgcttgt tgatcgcgac ctgctcggcg tgggccgcga
      361 ggttcgccgc gagcttcggc gtctgctccg acgcgtgtgc gacggcctcc tcggccgagc
      421 cgtacttctg gacgagcttc gcggcggtct tcgtgccgac gcccgggacc ccggggaggt
      481 tgtcggaggt gtcgccctcg agggccttga ggtccacgta cttcgcaggc gggacgccgt
      541 agcgctcctc gaccttcttg gggtccatct cgacgatgtc ggtgatcccg cgccggttgt
      601 agaggaccct gatgtccttg ctcacgagct ggaagaagtc gcggtcgccc gtgacgatcc
      661 gcacgtcgat cccctcggcc gcggccctct tcgcgaagca cgcgatcacg tcgtcggcct
      721 cgtggcccgg cacgccgaag atcgggatct gcatcacgcg caggacctcg taaatcagcg
      781 ggacctgccc ggagaagccg tcgggggccg tgctgcggtt gctcttgtac tcggcgtact
      841 ccgcggagcg ttccagcggc tctccgacgt cgaagcacac ggtgatgtac tcgggcttct
      901 gctcctggag cagcttgatc agcatcgccg tgaacccgta gaccgcgttc gtcttggtcc
      961 cgtcggtcgt ctgcaggtcc tccggcagcg cgtagaaggc gcggaaggcc agcgaatagc
     1021 cgtcgaggat cagcatcttc gagcgcttgc gcgacttcgt cggggtcttc gaggggggca
     1081 tctggggcga gcttacaatc ggcccgatga gccactcctc gcccgacctg cgcgcgctga
     1141 accccgcgtc gaaggagtac ctcgaggccg tgttcgagct cggcgaggag ggccagcgga
     1201 tcctgcaggc ccggatcggc gagcgcctgg ggctcgctcc ggcgacggtc tcggagggga
     1261 tcaagcggct cgtcggcgag ggctacgtga cggtcgactc gtcgcgccag atctccctga
     1321 cggacgaggg ccggacggtc gccgaggcgc tcgtgcgccg gcaccgcctg gccgagcgga
     1381 tgctcgtcga catcctcggg atcccgtggc acctctgcca ccagcaggcg gaggactggg
     1441 agaaggtcat gacgcccgag gtcgagcagg cgatcctggc gaagctcgac ggcgaggcga
     1501 cttgccccca cggcaacccg atccccgggg agccctcgtc ggtcccctgg tcggacctga
     1561 agtcggtcgc gtcgatgacc gtcggcgagc ggggccgcct caagcggctg ctggaggacg
     1621 tcgagctcga cggcgaggtg ctcgaatacc tcgagcagca ccggctgatg ccgggctacg
     1681 agctcgagct ggtcgaggtc gcccccgacg ggacgcgcgt gctggaggtc gacggcgacc
     1741 gggtcgcgct cggcacgcgg ctggcggaca acctctgggt gctcgtcgcc tgacttcctt
     1801 cgacttgatc caggcggccg ttagggtcgc ccgaaagcca accaacggag gtgctcgatg
     1861 accctgcgaa tcgccgcggc gctgatcgtc gcgctgctcg ccttttccgc ctgtgactcc
     1921 gacgacggcg cgtcggtccg cgacctcggc ggttccgcgt cggggtccgg gtcgggctcg
     1981 ggctccggga ccggtgccgc ctcgggctcc ggctcggcga ccgaggccga ggccgaggcg
     2041 tgcgagcccg gcgacgaggc ggaccccgcg ctcgtcgtga ggctcgacga gtactcggtc
     2101 gtcccggtgg aggagaagct gacgtcgggg cccacgcgct tccgcgccgt caacgcgggc
     2161 gaggaggtcc acgagctcta cctcgccgag ggcaagtcgc tggagggctt gccgctcgac
     2221 cccaagggct cggtcgacac cgaggcgctc gaggaggacg ggcggctgct cggcgaggtc
     2281 gagggcatcc cgtcgggaga gtcgtgcgac ctcgaggccg atctcgagat cgggacctac
     2341 gtgttgttct gcaacatccg cgagaggacc gacgagggcg tcgtgaacca cttcctccag
     2401 gggatgcgcg cccgcgtcaa ggtcgacgcg tagcgcccca ccacagcgcc gcgagggccg
     2461 caacgatcca cagcgcgtgc agcacccacg tcgcgcccgt cggggcgcgg tactcgggca
     2521 ggagccacga ccacgcgcgg tacagcgggt tcgacgtctc gtagaagtcg acgacgagcg
     2581 tgagccggcg cgcgagcgcc tcttgcgcga gccacgccca cgacacgacg cctgcggccc
     2641 ccagaccggc ggcacctagg agccgcgggc gcgaccgtgc ggcccaggcg gcgaccgcta
     2701 tcacgagcag cggcgctacg accacgacct gccggccggg gaaccaccag ccgtgcatcg
     2761 tcagcgcgac gaaggtcgcg ttcagccagc cggccgcggc cgggacgagc aggaacgccc
     2821 agccgagcgg acgtctcgcc gcgagggccg cggccgcggg gaccgcgaac agccacgccg
     2881 gctgccatgc ggcgaggccg aactcgcggt cgaccaggag cccgatcaga cgcgtgcttc
     2941 gtccagctag gttcgggtcg aacccgacga cgccgagctc tccggtgacg aagtggtcgc
     3001 cgaccgcgta cggggtccag cccctgtacc aggcccagtg cgcggctacg aagagcattc
     3061 cgctcagcgt gaggagcgcg cccagccatg cgacgtcccg gcgctcgccg cgccgcc
//
LOCUS       DASQDO010000278         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_579833, whole genome shotgun sequence.
ACCESSION   DASQDO010000278 DASQDO010000000
VERSION     DASQDO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579833"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VEV43_07805"
     CDS             <1..1123
                     /locus_tag="VEV43_07805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP23461.1"
                     /translation="YARVLREVLPRLTPDLDVTLFALNTRDAAATSSLPGVRVVVNGL
                     LGDPYGREQLPRLVDETQPDVVLLHHDLPLYSVFHEGLAEYRAARPHFRVIAYCPIEW
                     EGTSEGNVRTLAGVDAAVTYTEFGARAVERAFAGRADAPRVAVIGHGIDTSTFRPVAG
                     ARRALWPDRPELEDAFVVLNANRNVPRKKIDLTMSAFAEFAAGKPDTYLYLHMGMLDN
                     GIDVPVHARELGITDRLLVTTDDQAPPKIGDEELNLVYNACDVGLNTATGEGWGLVAF
                     EHGATGAAQVMPDHSACAELWRDRALFAPVEPGREEDGLVSRDGVVAALERLYADGGL
                     RARLGEAAREHALDPSFDWDTIAQSWRALLLDGPPRRDA"
     gene            1165..1458
                     /locus_tag="VEV43_07810"
     CDS             1165..1458
                     /locus_tag="VEV43_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23462.1"
                     /translation="MPDRLELSQFQPYVGHAFGVETAAGPLELTLAEASAGPWQPEGE
                     TAFAFELIFTGPREPVLPQAIYRMTHPDAGDHDIFIVPLKTAEDGTTYQAVFS"
BASE COUNT          235 a          546 c          550 g          226 t
ORIGIN      
        1 ctacgcgcgc gtcctccggg aggtgctgcc ccggcttacg ccggatctcg acgtcacgct
       61 gttcgcgctc aacacccgcg acgccgcggc gacgtcgtcg ttgccgggcg tgcgggtcgt
      121 agtcaacggc ctcctcggcg atccctacgg acgcgagcag ctcccccgcc tggtcgacga
      181 gacgcagccc gacgtcgtgc tcctccacca cgacctcccc ctgtactcgg tgttccacga
      241 agggctcgcc gaataccgtg ccgcccgccc tcacttccgc gtgatcgcgt actgcccgat
      301 cgagtgggag ggcacgagcg agggcaacgt ccgcacgctg gcgggggtcg acgccgcggt
      361 cacgtacacg gagttcggtg cccgcgccgt cgagcgggcg ttcgccggcc gcgccgatgc
      421 gccccgggtc gcggtcatcg gccacgggat cgacacctcg acgttccgtc cggtcgcggg
      481 tgcgcggcgc gcgctgtggc cggacaggcc cgagctcgag gacgcgttcg tcgttctcaa
      541 cgcgaaccgc aacgtgcctc gcaagaagat cgacctgacg atgtcggcgt tcgccgagtt
      601 cgccgcgggc aagcccgaca cgtacctgta cctccacatg gggatgctcg acaacgggat
      661 cgacgtcccg gtgcacgcga gggagctcgg catcacggac aggctgctgg tgacgacgga
      721 cgaccaggcg cccccgaaga tcggcgacga ggagctgaac ctcgtctaca acgcctgcga
      781 cgtcggcctg aacaccgcga cgggagaggg gtggggcctg gtcgcgttcg agcacggcgc
      841 tacgggggcc gcccaggtga tgccggatca cagcgcctgt gccgagctgt ggcgggacag
      901 ggcgttgttc gcccccgtgg agccgggccg ggaggaagac gggctcgtgt cccgcgacgg
      961 cgtcgttgct gcgctcgagc ggctctatgc cgacgggggg ctgcgggccc ggctgggcga
     1021 ggccgcgcgg gagcacgcgc tcgacccgtc gttcgactgg gacacaattg cgcagtcgtg
     1081 gagagcgctc ttgctcgacg gaccgcctcg gcgggacgcc tgaaagaaga cgacggaacg
     1141 ccactgagag aacggaggcc gccgttgccc gatcgtctcg agctgtcgca gttccagcca
     1201 tacgtgggcc acgcgttcgg agtcgagacg gcggccggcc ccctcgagct gaccctcgcg
     1261 gaggcgtcgg cgggcccctg gcagccggag ggcgagaccg cgttcgcgtt cgagctgatc
     1321 ttcaccggcc cgcgggagcc ggtccttcct caggcgatct accgcatgac gcatccggac
     1381 gcgggcgacc acgacatctt catcgtcccg ctgaagaccg cggaggacgg cacgacctat
     1441 caggcggtct tcagctagga gcggtccagc gcatcagcct gtagacgccg tgctcgccga
     1501 cctcgacgaa cccgaggcgg cgatacagag acatcgcccg gttcgtctct tcgacgt
//
LOCUS       DASQDO010000279         7662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_701966, whole genome shotgun sequence.
ACCESSION   DASQDO010000279 DASQDO010000000
VERSION     DASQDO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701966"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VEV43_07815"
     CDS             <1..700
                     /locus_tag="VEV43_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HYP23463.1"
                     /translation="AGRMGAGYVTLVSTSSVTASATVVAPEILSRVVPGEVLGPEALD
                     GAEGAGCLALGPGIGTGGPQRALCERALLEVDRPLVLDADALNVLAENVRPLVARQSP
                     AVLTPHPAELARLLGWPTKDVVKDRLAAASRAASGLGCVVVAKGHRTVVTDGDRSVVV
                     PTGGAELATAGTGDVLPGAVAALLAAGVDPFDAAWCAAYVHGLAGSLAGPGALAPDVA
                     EALGKARARFTLGS"
     gene            697..1887
                     /gene="alr"
                     /locus_tag="VEV43_07820"
     CDS             697..1887
                     /gene="alr"
                     /locus_tag="VEV43_07820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYP23464.1"
                     /translation="MSFTKEEIAARGHPVWAEIDLDAIRHNVSVLRRMASSSELMGVV
                     KGYAYGHGNPESALAMIDAGATRIGVARVAEAIHLREAGVEVPIHVFTEPPPVAAPTM
                     VDLRLTATVYTAPFAAALSEAASEAGARLRVHVKLDTGMHRVGLPPDEALDAVTLISK
                     LPGLSIEGAWSHLAVADVPDHPFTRKQLALFSELTDRIERGGLRLRYRHIANSAATMS
                     LPEARLDLVRCGIACFGLWPGPAFADTSDLRPALALKARVNLVKAVPEGEAVSYGLEY
                     KVPRDVSVVTVPAGYADGYDRRLSGRAPIVIDGGRYVVSGTVCMDQFMVDVGSDEVEV
                     GDTATLIGEGITAEELADIIGTINYEVTTRIPSRVPRVFLNTSGGLGRSDPASGGLGR
                     SDPE"
     gene            1884..2933
                     /locus_tag="VEV43_07825"
     CDS             1884..2933
                     /locus_tag="VEV43_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP23465.1"
                     /translation="MKKQGVFALAGAAAGVAAGLATQRTLVKRRRRNDPEAGERFGTR
                     RGVRPRTISLSDGADAFIEETGPESRKGAVFVHGSALRTDLWHYQLPGLGDHRLVFYD
                     LRGHGVSREKGSARFSIAQLARDLVEVIDDCGLDEVVVVGHSIGGMVALDLAATRADL
                     LGARVKGIVLVNTTYRPGVETVAGGAALAHVERLLRRPLDVVGKRAPTIDRLRAVVKP
                     SDLLFLTVAYTGFGPGAPASQVDFVYDMLAETPSDVIFDLISSYRDFDVTEVLGEVTV
                     PALVIGGTNDRITVPAASEYLAEHLPKAELKMFDRCGHMPMLERHREFNAIVASFLDD
                     TLGRPIAQEGEPEPS"
     gene            2930..4036
                     /locus_tag="VEV43_07830"
     CDS             2930..4036
                     /locus_tag="VEV43_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HYP23466.1"
                     /translation="MRDTRVEKLADILVDYSCRVQKGDLVGITASYQAEPLMLALYRR
                     CLDRGAHVMVRPSLPHAAPIFYNHASDEQLEFVWETDKWLAENLDVRFTIIADNNTRQ
                     LSKVDPRKQALTSRSRKPLMDVFMERGASGDLRWNVTLFPTEAHAMDAEMSLEEYEDF
                     YYGACLVDAEDPVAEWKKVGERHARMIDWIKDHNEIHIEGEGTDLILETGGRTWLPAD
                     GTHNFPDGEIFTGPHEDKTRGRVSFTYPAIYGGQAVEGIQLVFDEGKVVEATARSNEE
                     FLLKTLDTDPGARVLGELGIGTNYGIPGFSGEILLDEKIGGTIHLAVGAAYPESGGVN
                     DSAVHWDMVCDLRKGGRITVDGEVFMEDGKLLIS"
     gene            4046..4774
                     /locus_tag="VEV43_07835"
     CDS             4046..4774
                     /locus_tag="VEV43_07835"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYP23467.1"
                     /translation="MDIRAVEQQALGCVKCRLSETRTQVVWADGNFDSKFLLIGEAPG
                     RNEDLQGRPFVGAAGQLLDRLLAEVGLDRGRVAIANVIKCRPPGNRDPLPDEIETCSP
                     WLQAQLAYMQPDVIVTLGNFATKFVLQSPVGITRARGQVYQRNGSAIVPTFHPAAALR
                     GGRFGGMDPTDAIRADLKVAAAELAAAAARATVPPEPEPGLEVVPRETASAGSQDQRE
                     TASAESQDHRETASAGSQDQMGLF"
     gene            4774..5346
                     /gene="tsaE"
                     /locus_tag="VEV43_07840"
     CDS             4774..5346
                     /gene="tsaE"
                     /locus_tag="VEV43_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYP23468.1"
                     /translation="MRRGIGVTSRSPEETRILGACLAPVLLPGDVVSLSGDLGAGKTV
                     FVQGVCSALGYSGRVTSPTFTLVNEYDARYPIVHLDVYRLDSFQEVLDLGFEELIGPD
                     SIVLVEWGEAVSPLLPKRSLDVVLTRSDDLDATEERDVVFRPGSPDWIGKLEALRTTA
                     EALLNAAAPDATGSPRFWDAGRAKAAGGEA"
     gene            5346..6056
                     /gene="tsaB"
                     /locus_tag="VEV43_07845"
     CDS             5346..6056
                     /gene="tsaB"
                     /locus_tag="VEV43_07845"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HYP23469.1"
                     /translation="MLVLGIECSTPQASVAIGSEQGVVASALVSRGATHNEFLLPAIR
                     FCLDEAGLGYRNLGGLAVSLGPGLFSGMRVGVATAKALAQTLSVPIVGMASLDLVAYE
                     VRHTSKTICAVLDARRNEVFYAFYRPSPGGIQRMTDYRIEQPGRLAIGIASRPEEVLL
                     VGNGALLYKELFESAGPVVEVAGMNQSFPNACALVELALPRLYREDFDSLYDLRPLYL
                     RKSAKRIEWDRIRQSRSA"
     gene            6056..6649
                     /gene="rimI"
                     /locus_tag="VEV43_07850"
     CDS             6056..6649
                     /gene="rimI"
                     /locus_tag="VEV43_07850"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HYP23470.1"
                     /translation="MVLARLPDRGSIPDVELTKMRRRHLRKVLAIEARVYPRPWSASL
                     FLSELAQKSSRSYLVARHEGEVVGYAGMMYTGLEAHITNIAVDPDFHGRKVGSRLMLR
                     LVTEAIARGARTLSLEVRVTNLPAQTMYGKFGFSVVGVRKGYYIETKEDALVMVVDDA
                     TTNEYRLRLQTLRDEIDMVTGDDGSSRRSARNDDARG"
     gene            6630..>7662
                     /gene="tsaD"
                     /locus_tag="VEV43_07855"
     CDS             6630..>7662
                     /gene="tsaD"
                     /locus_tag="VEV43_07855"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYP23471.1"
                     /translation="MTMRVGETLWASPEVATEKKPVGEWDEETLVLGIETSCDETSAS
                     VVKGGRQILSNVIVSQGELHGEYGGIVPEVAARAHVEALSPAIGEALLQADATFWDLD
                     AVAATRGPGLVGSLLVGVAEAKSIAAVLEVPFLAVNHLEAHLYSALLVEPDATFPAVA
                     LIISGGHTLIVHAVNHGAYEILGGTLDDAAGEAFDKVARFLGLDYPGGPEIDRLSLLG
                     DPAAIRFPRAMMNEPGFDVSYSGLKTSVINYVRKQEASGEAISVEDIAASFQQAVVDV
                     QVAKTMAAVEPAGVDRLFLVGGVAANSGLRSGMAAACEAANVKLTVPTMELCTDNAAM
                     IAACGRAMLR"
BASE COUNT         1122 a         2669 c         2732 g         1139 t
ORIGIN      
        1 cgccggacgc atgggggccg gttacgtgac gctcgtctcg acttcctccg tcaccgcgtc
       61 cgcgacggtc gtcgcgcccg agatcctgag ccgcgtggtt ccgggggagg tgttggggcc
      121 cgaggctctc gacggtgccg agggggccgg gtgcctcgct ctcgggcccg ggatcgggac
      181 gggtggtccc cagcgcgcgc tctgcgagcg ggcgctgctc gaggtcgacc gaccgctcgt
      241 tctcgatgcc gatgccttga acgtcctcgc cgagaacgtc cggcctctcg tcgcgcggca
      301 gtcgcccgcg gtcctcacgc ctcatcccgc cgagctcgct cggttgttgg ggtggcccac
      361 caaggacgtc gtcaaggatc gtcttgctgc cgcgtccagg gccgcctccg gcctcggctg
      421 cgtcgtcgtc gccaaaggcc atcgcaccgt cgtcaccgac ggtgaccgca gcgtcgtcgt
      481 gcccaccggg ggcgccgagc tcgcaaccgc cggcaccggc gacgtcctcc cgggtgcggt
      541 cgccgccctg ctcgcggcgg gggtcgaccc gttcgacgcc gcttggtgcg cggcctacgt
      601 ccacgggctg gccggatcgc tcgccggccc gggcgctctt gcccccgacg tcgccgaagc
      661 cctcggaaag gcgcgggccc ggtttacgtt ggggtcgtga gcttcaccaa ggaggagatc
      721 gcggcgcggg ggcatcccgt gtgggccgag atcgacctcg acgcgatccg gcacaacgtc
      781 tccgtgctcc gcaggatggc gtcgtcctcc gagctgatgg gcgtggtcaa gggctacgcc
      841 tacggtcacg ggaacccgga gagcgccctc gcgatgatcg acgccggcgc gacgcgcatc
      901 ggcgtcgccc gcgtcgccga ggcgatccac ctgcgcgaag ccggcgtcga ggtgcccatc
      961 cacgtcttca ccgagccccc gcccgtcgcc gcgccgacga tggtcgacct gcggctcacc
     1021 gcgaccgtct acaccgcgcc gttcgccgcg gccctctccg aggcggcatc cgaggccggc
     1081 gcccgcctcc gcgtgcacgt gaagctcgac accggcatgc accgggtggg gctcccgccg
     1141 gacgaggcgc tggacgccgt cacgctcatc tcgaagctgc ccggcctctc gatcgagggg
     1201 gcgtggagcc acctggcggt cgccgacgtg cccgaccatc cgttcacgcg caagcagctc
     1261 gcgctgttct ccgagctcac cgacaggatc gagcgaggcg ggctcaggct gcgctaccgg
     1321 cacatcgcga actcggccgc gacgatgtcg ctgccggagg cgcgcctcga cctcgtcagg
     1381 tgcggcatcg cctgcttcgg gctgtggccg ggccccgcgt tcgcggacac gtcggacctg
     1441 cgacccgcgc tcgcgttgaa ggcccgcgtg aacctcgtca aggccgtgcc cgagggtgag
     1501 gccgtctcgt acggcctcga gtacaaggtc ccgcgcgacg tgagcgtcgt gaccgtgccc
     1561 gctgggtatg ccgacgggta cgaccgccgg ctgtcggggc gggcgccgat cgtcatcgac
     1621 ggcggccgct acgtcgtgtc gggaacggtc tgcatggacc agttcatggt cgacgtcggt
     1681 tccgacgagg tcgaggtcgg cgacacggcg accttgatag gagaaggcat cactgcggag
     1741 gagttggcgg acatcatcgg gacgatcaac tacgaggtca cgacgcgcat accgtcccgg
     1801 gtcccgcgcg tgttcctgaa taccagcggc ggcctcggcc ggagcgatcc agccagcggc
     1861 ggcctcggcc ggagcgatcc agagtgaaga agcagggggt gttcgcgctg gcgggggccg
     1921 cggccggagt cgcggcgggg ctcgcgacgc agcgcacgct cgtcaagcga cgccggcgca
     1981 acgaccccga agcgggcgag cgcttcggca cccggcgagg ggtgcgcccg cggacgatct
     2041 cgttgtccga cggagccgac gccttcatcg aggagaccgg cccggagtcc cgcaaggggg
     2101 ccgtcttcgt ccacggctcc gcgctgagga ccgacctgtg gcactaccag ctgcccggcc
     2161 tcggcgacca ccgtctcgtc ttctacgacc tgcgcggcca cggcgtctcg cgcgagaagg
     2221 ggagcgcgcg cttctcgatc gcgcagctcg cccgcgacct cgtcgaggtc atcgacgact
     2281 gcgggctcga cgaggtcgtg gtcgtcgggc actcgatcgg gggcatggtg gcgctcgacc
     2341 tggccgccac gcgcgccgac ctgctcggtg cccgcgtcaa gggcatcgtc ctcgtcaaca
     2401 ccacctaccg tccgggcgtc gagaccgtcg ccggcggagc ggcgctcgcg cacgtcgagc
     2461 gtctcttgcg gcgccccctc gacgtcgtag gcaagagggc tccgaccatc gacaggctcc
     2521 gggccgtcgt caagccgtcg gacctgctgt tcctcaccgt ggcgtacacc gggttcggtc
     2581 ccggtgcgcc ggcgagccag gtcgacttcg tctacgacat gctggccgag acgccctcgg
     2641 acgtgatctt cgacctcatc agctcgtacc gcgacttcga cgtgaccgag gtgctgggcg
     2701 aggtgacggt cccggcgctg gttatcgggg ggacgaacga ccgcatcacg gttccggcgg
     2761 cctcggagta tcttgccgag cacctgccga aggcagagct gaagatgttc gaccgctgcg
     2821 ggcacatgcc gatgctggag cgccaccgcg agttcaacgc gatcgtcgcg tcgttcctcg
     2881 acgacacgtt aggacgacct attgcccaag aaggggagcc ggaaccatca tgagagacac
     2941 cagggtcgag aagctggccg acatactcgt cgactactcc tgccgcgtgc agaagggcga
     3001 cctcgtcggc atcaccgcga gctaccaggc cgagccgctg atgctcgcgc tctaccggcg
     3061 ttgcctcgac cgcggcgcgc acgtgatggt gcgtccgtcg ctaccgcacg cggcccccat
     3121 cttctacaac cacgcgagcg acgagcagct cgagttcgtg tgggagaccg acaagtggct
     3181 ggccgagaac ctcgacgtcc gcttcacgat catcgccgac aacaacacgc ggcagctctc
     3241 gaaggtggac ccgcgcaagc aggcgctgac gtcgcgctcg cgcaagccgc tgatggacgt
     3301 cttcatggag cggggggcgt ccggcgacct caggtggaac gtcacgctgt tcccgaccga
     3361 ggcccacgcc atggacgccg agatgagcct cgaggagtac gaggacttct actacggcgc
     3421 gtgcctggtg gacgcggagg accccgtcgc cgagtggaag aaggtcggcg agcgccacgc
     3481 ccgcatgatc gattggatca aggaccacaa cgagatccac atcgagggcg agggcaccga
     3541 cctcatcctc gagaccggcg ggcgcacgtg gctgccggcg gacggcaccc acaacttccc
     3601 cgacggcgag atcttcaccg gcccccacga ggacaagacg cgcgggcggg tctcgttcac
     3661 ctatccggcg atctacggcg gccaggcggt cgaggggatc cagctcgtgt tcgacgaggg
     3721 gaaggtcgtc gaggcgacgg cgcgctcgaa cgaggagttc ctcctgaaga ccctcgacac
     3781 cgaccccggc gcacgcgtgc tcggcgagct cggcatcggg accaactacg gcatcccggg
     3841 gttctcgggc gagatcctgc tcgacgagaa gatcggcggg acgatccacc tcgcagtcgg
     3901 cgccgcctac ccggagagcg gcggcgtcaa cgactcggcg gtgcactggg acatggtctg
     3961 tgacctgcgc aaaggggggc ggatcacggt cgacggcgag gtgttcatgg aggacggcaa
     4021 gctcctcatc tcgtaacctc ggcccgtgga catccgcgcg gtcgagcagc aggccctggg
     4081 gtgtgtcaag tgcaggctct ccgagacgcg gacccaggtc gtgtgggccg acggcaactt
     4141 cgactcgaag ttcctgctga tcggcgaggc ccccggccgc aacgaggacc tgcagggccg
     4201 ccccttcgtc ggcgccgccg ggcagctgct cgaccggctg ctcgccgagg tcgggctcga
     4261 ccggggccgg gtcgccatcg ccaacgtgat caagtgccgg ccgccgggca accgtgaccc
     4321 gctgccggac gagatcgaga cgtgctcgcc gtggctccag gcccagctcg cctacatgca
     4381 gcccgacgtg atcgtgaccc tcgggaactt cgccacgaag ttcgtgctcc agtcgccggt
     4441 cggcatcacc cgcgcccggg gacaggtgta ccagcgcaac gggtccgcga tcgtgccgac
     4501 cttccacccg gcggcggcgc tgcggggcgg gcggttcggg ggcatggacc cgaccgacgc
     4561 gatccgggcg gacctgaagg tggcggcggc cgagctggcg gcggccgcgg cccgggcgac
     4621 cgttccgccc gagccggagc cggggctcga ggtcgtaccg cgcgagaccg cctcggcggg
     4681 atcgcaagac cagcgcgaga ccgcctcggc ggaatcccaa gaccaccgcg agaccgcctc
     4741 ggcgggatcc caagatcaga tgggcctctt ctgatgcggc gggggatagg cgtcacgagc
     4801 cggtcgcccg aggagacgcg catcctcggc gcgtgcctgg cccccgtcct cctccccggc
     4861 gacgtcgtca gcctctcggg cgacctcggc gccggcaaga ccgtcttcgt ccagggggtc
     4921 tgctcggccc tcggctactc gggccgggtc acgagcccca cgttcacgct cgtcaacgag
     4981 tacgacgccc gctacccgat cgtgcacctc gacgtctacc ggctcgactc cttccaggag
     5041 gtgctcgacc tcggcttcga ggagctcatc gggcccgact cgatcgtgct ggtcgagtgg
     5101 ggtgaggccg tctcgccgct ccttcccaag aggtcgctcg acgtcgtgct cacgcgctcc
     5161 gacgacctgg acgcgaccga ggagcgcgac gtcgtgttcc gcccggggtc gcccgactgg
     5221 atcggcaagc tcgaggcgtt gcggacgacc gccgaggccc tcctcaacgc ggcggcgccg
     5281 gatgcgaccg ggagcccccg cttctgggat gctggacggg ccaaagctgc gggaggggaa
     5341 gcctgatgct cgtcttgggg atcgagtgct cgacgcctca ggccagcgtg gcgatcggct
     5401 ccgagcaagg tgtcgtcgcc agcgcgctgg tctcgcgcgg cgcgacccac aacgagttcc
     5461 tgctgcccgc gatccgcttc tgcctcgacg aggccgggct cggctatcgc aatcttggag
     5521 gtctcgcggt cagcctcggg ccggggctct tctcgggcat gcgcgtcggc gtcgcgaccg
     5581 cgaaggccct ggcgcagacg ctgtcggtcc cgatcgtcgg catggcgagc ctcgacctcg
     5641 tcgcgtacga ggtgcgccac acatccaaga cgatctgcgc ggtcctcgac gcgcggcgca
     5701 acgaggtctt ctacgcgttc taccgtccgt cgcccggcgg catccagagg atgaccgact
     5761 accgcatcga gcagccgggc cggctcgcga tcgggatcgc gtcgcggccc gaggaggtgc
     5821 tgctcgtcgg caacggcgcg ctgctctaca aggagctgtt cgagtcggcc gggccggtcg
     5881 tcgaggtggc cgggatgaac cagtcgttcc cgaacgcgtg tgcgctcgtc gagctcgcgc
     5941 ttccgcgcct gtaccgagag gacttcgact cgctctacga cctccgcccg ctgtacctgc
     6001 gcaagtccgc gaagcggatc gagtgggacc gcatccggca gagcagatcg gcctgatggt
     6061 gctcgcacgg ctcccggatc ggggttcgat ccccgacgtc gagctcacga agatgcgtcg
     6121 ccgtcacctc cgcaaggtgc tggcgatcga ggcgcgcgtg tacccgcgtc cgtggagcgc
     6181 gtcactgttc ctgtcggagc tcgcgcagaa gagctcgcgc tcgtacctcg tcgcgcgtca
     6241 cgagggcgag gtcgtcgggt acgccgggat gatgtacacg gggctcgagg cgcacatcac
     6301 gaacatcgcc gtcgacccgg acttccacgg ccgcaaggtc ggctcgcggc tgatgctgcg
     6361 gctcgtgacc gaggcgatcg cccggggggc gcgcacgctg tcgctcgagg tgagggtgac
     6421 gaacctcccc gcgcagacca tgtacgggaa gttcggcttc tcggtcgtcg gagtgcgcaa
     6481 gggctactac atcgagacga aggaggacgc gctggtgatg gtcgtcgacg acgcgacgac
     6541 gaacgagtac cggctgcgtc tccagaccct gagggacgag atcgacatgg tcacgggtga
     6601 cgacggatcg tctcgacgat ccgccaggaa tgacgatgcg cgtgggtgag accttgtggg
     6661 cctcgcccga ggtcgcgacc gagaagaagc ccgtcggcga atgggacgag gagacgctcg
     6721 tcctcggcat cgagacgtcg tgcgacgaga cgtccgcgtc ggtcgtcaag ggcgggcggc
     6781 agatcctctc gaacgtcatc gtgtcgcagg gcgagctgca cggcgagtac gggggcatcg
     6841 tcccggaggt cgcggcccgc gcacacgtcg aagccctgtc gcctgcgatc ggcgaggccc
     6901 tgctccaggc agacgcgacg ttctgggacc tcgacgcggt cgccgcgacg cgggggccgg
     6961 ggctcgtggg ctcgctgctc gtgggcgtcg cggaggcgaa gtcgatcgcc gcggtgctcg
     7021 aggtgccgtt cctcgcggtc aaccacctcg aggcccacct gtactcggcc ctgctggtcg
     7081 agcccgacgc gacgttcccg gcggtcgcgc tgatcatctc cggcggccac acgctgatcg
     7141 tgcacgcggt gaaccacggt gcgtacgaga tcctcggcgg gaccctcgac gacgccgcgg
     7201 gcgaggcgtt cgacaaggtc gcccggttcc tcgggctcga ctacccgggg gggccggaga
     7261 tcgaccggct ctcgttgctc ggcgaccccg cggcgatcag gttcccgcgg gcgatgatga
     7321 acgagccggg cttcgacgtc tcgtactcgg ggctcaagac ctcggtgatc aactacgtcc
     7381 gcaagcagga ggcctcgggc gaggcgatct cggtcgagga catcgcggcg tcgttccagc
     7441 aggccgtcgt cgacgtccag gtggcgaaga cgatggccgc ggtcgagccc gcgggcgtcg
     7501 accggctctt cttggtcggc ggggtcgcgg cgaactcggg gctgcgctcg gggatggcgg
     7561 ccgcctgcga ggcggcgaac gtgaagctca cggtcccgac gatggagctc tgcacggaca
     7621 acgcggcgat gatcgccgcc tgcgggaggg cgatgctgcg cc
//
LOCUS       DASQDO010000280         5623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_152263, whole genome shotgun sequence.
ACCESSION   DASQDO010000280 DASQDO010000000
VERSION     DASQDO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152263"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VEV43_07860"
     CDS             complement(<1..603)
                     /locus_tag="VEV43_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23472.1"
                     /translation="MDVKLDRLVVAVVIAVMGAALPAGASHDEDVHSDNMKLVGNAPL
                     EGATDVEFTKDGYAVVAVNGSGEGAGLWTVDVRNPKKPKPVGHLPCAGSGYDVGLWRD
                     IAVMSIDAASGNSSTKEDGCNLDGTENTEGIRLVDVSNRRKPVEVAFVETACGSHTNI
                     TFEHEGRGLVYVQSYPASTSGACPSAHGIISVVDITNPRAP"
     gene            633..1826
                     /gene="hemW"
                     /locus_tag="VEV43_07865"
     CDS             633..1826
                     /gene="hemW"
                     /locus_tag="VEV43_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HYP23473.1"
                     /translation="MPLHLSDSPDSSPALLDPGAWRADPGYGVYVHVPFCVHRCHYCD
                     FNTYEGLGELHEPYAAALEREIAGTQGERGPATSVFFGGGTPTLLSPATLRRLLDAVR
                     GSIGIAAGAEVTIEANPETVDEETFEALLDAGFNRFSVGVQSLVPNVLQRLGRTHSGE
                     TALAALAAAKRAGVEDVNADLIFGSPWETSDDWRRSLDGIVAAGTSHVSAYALTIEEG
                     TPLATLVSTGRVPDVDPDVQAERHATATEVLGAAGFVRYEVSNWARPGHASRHNVLYW
                     SAGDYAGFGAGAHGHEAGRRYWRTRLPRDYVSEAESGATTEAGEERLTPEERAREAMT
                     LGLRLVSGVEEDAFYARFPAAEPAVRATADELCREGCLVRAGGWLRLPEHATFVANDV
                     LCRFL"
     gene            1823..2320
                     /locus_tag="VEV43_07870"
     CDS             1823..2320
                     /locus_tag="VEV43_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HYP23474.1"
                     /translation="MKPYVAVVGAGRAPAALDRVAEDVGRGLGEQGAVVLCGGLSGVM
                     EAACRGCRAAGGTSVGVLPGDDRAEANEFVDVALATGMGEMRNALIVRAADVVVALGG
                     EYGTLSEVAFALKIGRPVVGYDTWELSKDGEPRDGIVRVGSAEEAVATALRLCSRRSD
                     GSSGR"
     gene            2325..2516
                     /locus_tag="VEV43_07875"
     CDS             2325..2516
                     /locus_tag="VEV43_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23475.1"
                     /translation="MKLRRAILATIAASTMALVPSASASPYNELCGDGTPHGCILHCM
                     QYHGGLKNLQNCFTYHYLV"
     gene            2675..2902
                     /locus_tag="VEV43_07880"
     CDS             2675..2902
                     /locus_tag="VEV43_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP23476.1"
                     /translation="MPFTTDELDVIELIANGHTDRAIARRLSVSVVTVRRRATSFRTK
                     IGASNRAEAIAIAAGRGWFEHPAVRAARDRG"
     gene            2976..>5623
                     /locus_tag="VEV43_07885"
     CDS             2976..>5623
                     /locus_tag="VEV43_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23477.1"
                     /translation="MRRVALVLLTGLVVAASTGAAPANPCPGVTAAGSWKTIETPMPT
                     AAFTVDPAGRIFVAGGRTIAVSIDGGCAWADALRINELVPGPVGERRVTSLATVGRSV
                     VGAVTGGPFVVVSNDGGETWRVSSIGLDVPGDPAGLYAAGGVVYLLVRREATDETVAG
                     PGVSAAVTVVYRSDDGGVTWTRGDAIATAYTGPQGSGVEGASAPGAVWDLAVDPGDPD
                     HVLAASRGGVFRSVDGGRSWASAMPEPGSDVRAVTIWRTGAVPAAAAVDPSSGTLYEG
                     DVVAGTWRRKVVPRLRTEMMTQAPNAAAWAWAAAKPSGELFLSGPNGVFRVAGRTLAD
                     VTPPGTAEGTVAGVRVFGGAVWGRLLDGSALVTQVGLSAGRPGGDSPAGAGTPGGDPI
                     ERAIARLPDPPVPGGRAHLEPGAKRVELAPGETRAVKFEARLGPRPVDLDLYFLVDTT
                     ASMSRTIRGLREGLGEIVLRLARSRIRLRVGVGAFRTYPRETDGASVEYAYRRMRALG
                     PLDSELLRVLYELDGAGSSGANLTALYQAVTGAGQDVLPPGPSNADVPAGGEAGFREG
                     AMKVVVHFADTWFGTPERGDPTNRYPPGTWPGPPMETAADALRDEGVMHLGVALQPAG
                     SRSVIDDADVVQDMRTMSRATSSFAGARGVDCDGDAEPDVPPGEALVCLLDRDADAGG
                     VASVVTGLVKAIERRGDVRLAEARESALVRAISPPVHGEVDLRVPQRLAFDVTVGCRR
                     SDAGTNTAGFDLLVAGARVAGSSLDVVCTEPPAPALSTRPDTPSPSALAAIPPIPVLP
                     PPHPVPGPGPGSVTAPAPVQAPAPAQAPGGQPSATVVAQRQQQPQVAFVTAVQQVREQ
                     TQMQHAFVRTRSRDPLAAARLWTAVGA"
BASE COUNT          741 a         2030 c         2067 g          785 t
ORIGIN      
        1 cggagcgcgg gggttcgtga tgtcgacgac cgagatgatc ccgtgcgcgc tcgggcacgc
       61 gccgctcgtc gacgccgggt acgactggac gtatacgagc ccgcggccct cgtgctcgaa
      121 cgtgatgttc gtgtggctcc cgcacgcggt ctcgacgaac gcgacctcga ccggcttgcg
      181 gcggttcgag acgtcgacca ggcggattcc ctcggtgttc tcggtgccgt cgaggttgca
      241 gccgtcctcc ttggtcgacg agttgccgga cgcggcgtcg atgctcatca ccgcgatgtc
      301 ccgccacagc ccgacgtcgt agccggagcc cgcgcaggga aggtgcccga ccggcttcgg
      361 cttcttcggg ttgcgcacgt cgacggtcca gagcccggcc ccctcgcccg agccgttgac
      421 cgcgacgacc gcgtagccgt ccttcgtgaa ctcgacgtcg gtcgcgccct cgagcggggc
      481 gttgccgacg agcttcatgt tgtccgagtg gacgtcctcg tcgtgcgacg ccccggcggg
      541 cagggcggcc cccatcacgg cgatcaccac ggcgaccacg agacgatcga gcttcacgtc
      601 cacgtcactc caatccctaa gatgggtccg ccgtgcccct ccacctgagt gattcgccgg
      661 attcgtcccc cgccctcctc gacccggggg cctggcgcgc cgaccccggc tacggcgtct
      721 acgtccacgt cccgttctgc gtccaccgct gccactactg cgacttcaac acctacgaag
      781 gtctcggcga gctccacgag ccgtacgccg ccgcgctgga gcgcgagatc gccggaacgc
      841 agggagagcg tggcccggca acctccgtct tcttcggcgg cgggacgccc acgctcctga
      901 gcccggcgac gttgcgccgg ctcctcgacg cggtccgggg ctcgatcggg atcgccgcgg
      961 gggcagaggt gacgatcgag gcgaacccgg agacggtcga cgaggagacg ttcgaagccc
     1021 ttctcgacgc cgggttcaac cgcttctccg tcggcgtgca gtcgctcgtc ccgaacgtcc
     1081 tgcagcgtct gggccggacg cactccggcg agaccgcgct ggccgccctc gccgcggcga
     1141 agcgcgcggg ggtcgaggac gtgaacgcgg acctcatctt cggatcgccg tgggagacct
     1201 cagacgactg gcgccgctcg ctcgacggga tcgtcgccgc gggaacctcc cacgtctccg
     1261 cgtacgcgct cacgatcgag gagggcacgc cgctcgcgac gctggtgtcg accggccgcg
     1321 tccccgacgt cgatcccgac gtgcaggccg agcggcacgc gaccgcgacc gaggttctcg
     1381 gcgccgcggg gttcgtccgg tacgaggtct cgaactgggc ccggcccggc catgcctccc
     1441 ggcacaacgt cctctactgg agcgcaggtg actacgcggg gttcggtgcc ggagcgcacg
     1501 gccacgaagc cggacgcagg tactggcgca cgcgcctgcc gcgcgactac gtctccgaag
     1561 ccgagagcgg cgccaccacc gaagcgggcg aggagaggct gacgccggag gagagggcgc
     1621 gcgaggcgat gaccctcggc ctgcgtctcg tctccggtgt cgaggaggac gccttctacg
     1681 ccaggttccc cgcagcggag cccgcggtgc gcgccacggc ggacgagctg tgccgcgagg
     1741 ggtgcctcgt ccgcgccggt ggctggttgc gcctgccgga gcacgcgacg ttcgtcgcca
     1801 acgacgtcct ctgccgcttc ctgtgaagcc ctacgtcgcg gtcgtcggcg ccggccgtgc
     1861 ccccgccgcc ctcgaccgcg tcgccgagga cgtggggcgc gggctcggcg agcagggcgc
     1921 agtagtgctc tgcggaggtc tgagcggcgt gatggaggca gcgtgtcgag gctgtcgcgc
     1981 cgccggaggc acgagcgtgg gggtccttcc cggcgacgac cgggccgagg cgaacgagtt
     2041 cgtcgacgtc gcgctcgcga cggggatggg cgagatgcgc aacgccctca tcgtgcgggc
     2101 ggccgacgtc gtcgtcgcgc tcggcggcga gtacgggacc ctgtccgagg tcgcgtttgc
     2161 gctgaagatc gggaggcccg tcgtcggcta cgacacgtgg gagctgtcga aggacggtga
     2221 gccacgcgac gggatcgtcc gcgtgggctc ggcggaagaa gcggtcgcga cggccctgcg
     2281 tctatgctcc cgccggagtg acggctcgag cgggaggtag gcggatgaag ctgagacggg
     2341 cgatcctcgc gacgatcgcg gcgagcacga tggcgctggt acccagcgcg tcggcgtcgc
     2401 cgtacaacga gctctgcggt gacggcacgc cgcatgggtg catcttgcac tgcatgcagt
     2461 accacggtgg cctcaagaac ctgcagaact gcttcacgta ccactacctc gtctgacgca
     2521 ccggtaagca tcgagacgcc tccttcggga ggcgtcttgc gcgtccgggt tggcgacccc
     2581 gtggccgccc gttggcgagc acttcacgcc gcgcgctccg catgctcgtc gagcacctcg
     2641 aggcgcgcga cgggaggcgg acatcgaaga ggacttgccg ttcacgacgg acgagctcga
     2701 cgtcatcgag ctcatcgcga acgggcacac ggaccgtgcg atcgcacgac ggttgtcggt
     2761 ctccgtggtc accgtgcgcc ggcgtgcgac gagcttccgc acgaagatcg gcgcgagcaa
     2821 ccgggcggag gcgatagcga tcgccgcggg ccgcggctgg ttcgagcacc cggcggtccg
     2881 cgccgcgcga gaccgaggtt gaagacatct actcagaggc gcggcccccg tccgagcgaa
     2941 ccctgagcga gacgccgacg aacgggggga ggcacgtgag gcgcgtcgcg ctggtgctgt
     3001 tgacagggct cgtcgtcgcc gcgtctaccg gcgcggcccc cgcgaaccca tgcccgggcg
     3061 tgactgccgc gggcagctgg aagacgatcg agacccccat gcccactgcg gccttcaccg
     3121 tcgaccccgc cggccggatc ttcgtcgcag gcggccggac gatcgccgtc tccatcgacg
     3181 gcgggtgtgc gtgggccgat gccctgcgga tcaacgagct cgtgccgggc cccgtcggcg
     3241 agaggagggt gacgagcctg gcgaccgtgg ggcgctcggt cgtcggcgcc gtcaccggcg
     3301 ggcccttcgt cgtcgtctcg aacgacggag gcgagacgtg gcgcgtgtcg tcgataggtc
     3361 tcgacgtccc gggagacccg gcgggcctct acgcggccgg cggcgtggtc tacctgctgg
     3421 tgcgccggga ggcaaccgac gagaccgtcg ccgggccggg cgtgagcgca gccgtgaccg
     3481 tcgtgtaccg cagcgacgac ggcggcgtga cgtggacgcg cggcgacgcg atcgcgacgg
     3541 cttacacggg cccgcagggc agcggcgtcg agggcgcgtc ggcgccggga gccgtgtggg
     3601 acctggcggt ggaccccggc gacccggacc acgtcctggc ggcgtcgcgc ggcggcgtct
     3661 tcaggtccgt cgacggtggc cgcagctggg cttcggcgat gccggagccg ggctccgacg
     3721 ttcgcgccgt gacgatctgg aggaccggcg cagtgccggc cgcggcggcg gtcgacccct
     3781 cgagcggcac gctctacgaa ggcgacgtcg tcgccgggac gtggcgccgc aaagttgttc
     3841 cccgcctccg caccgagatg atgacccagg ccccgaacgc tgctgcgtgg gcgtgggcag
     3901 cggcgaagcc gtcgggcgag ctcttcctgt cggggccgaa cggggtgttc cgcgtcgccg
     3961 gccgcacgct cgcggacgtc acgcctccgg gaaccgccga aggcaccgtc gccggcgtcc
     4021 gcgtcttcgg cggggccgtg tgggggcgtc tgctcgacgg gtccgcgctc gtcacccagg
     4081 tcggcctctc tgcgggccgt cccggcggcg actcgcccgc gggcgccggc acgcccggcg
     4141 gcgacccgat cgagcgggcg atcgcgcgcc tgccggaccc tcccgtaccc ggcggacgtg
     4201 cgcatctgga accgggcgcg aagcgcgtgg agctggcccc cggcgagacg cgcgcggtga
     4261 agttcgaagc gcggctcggg ccgcgcccgg tggacctcga cctctacttc ctcgtcgaca
     4321 ccacggccag catgagcagg acgatccgcg gcctccgcga gggcctgggc gagatcgtgc
     4381 tccgtctcgc acgttcgagg atccggctgc gagtgggcgt cggagcgttc cgcacctacc
     4441 cgcgcgagac cgacggcgcc tccgtcgagt acgcgtaccg acgcatgcgc gctctcggcc
     4501 ccctcgactc cgagctcttg cgcgtcctct acgagctcga cggggcgggg agctccggcg
     4561 cgaacctgac cgcgctgtac caggccgtca cgggggccgg gcaggacgtg ctcccgccgg
     4621 ggccgtcgaa cgccgacgtc ccggcgggcg gcgaggccgg cttccgcgag ggcgcgatga
     4681 aggtcgtcgt gcacttcgcc gacacgtggt tcgggacgcc cgaacgcggc gaccccacca
     4741 accgataccc gccggggacg tggccgggac ctcccatgga gaccgccgcc gacgcgctgc
     4801 gcgacgaagg ggtcatgcac ctcggcgtcg cgttgcagcc cgccggcagc cggagcgtca
     4861 tcgacgatgc ggacgtggtc caggacatgc ggacgatgtc gcgtgcaacc tcctcgttcg
     4921 ccggcgcccg aggtgtcgac tgcgacgggg acgcggagcc cgacgtaccg ccgggtgaag
     4981 ctctcgtgtg tctcctcgat cgcgacgcgg acgccggcgg ggtcgcgtcg gtggtcacgg
     5041 gcctggtcaa ggcgatcgag cgtcgcggcg acgtgcggct cgcggaggcg cgggagtctg
     5101 cgctcgttcg ggcgatctcc ccgcccgtcc acggcgaagt cgacctccgg gtgccgcaac
     5161 ggctggcgtt cgacgtcacc gtgggctgcc gcaggtctga cgcagggacg aataccgccg
     5221 gcttcgacct cctcgtcgcc ggagcccgcg tcgccggttc gagcctcgac gtcgtctgca
     5281 cggagcctcc cgcccccgcc ctctcgacac gccccgacac gccgtctccg tcagcgctcg
     5341 ccgccattcc tccgatcccg gtcctgccgc cgccccaccc ggttccaggt cccgggccgg
     5401 gatcggttac ggccccggcg cctgtgcagg cgcccgctcc tgcacaggcg cccggtggcc
     5461 aacccagcgc gacggtcgtc gcgcagcggc agcaacagcc acaggtcgcg ttcgtcaccg
     5521 cggtccagca ggtgcgcgag cagacgcaga tgcagcacgc tttcgtccgg acgcggtcgc
     5581 gcgacccgct tgccgcggcg cggctgtgga ccgcggtcgg cgc
//
LOCUS       DASQDO010000281         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_702575, whole genome shotgun sequence.
ACCESSION   DASQDO010000281 DASQDO010000000
VERSION     DASQDO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702575"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VEV43_07890"
     CDS             <1..430
                     /locus_tag="VEV43_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HYP23478.1"
                     /translation="DFGRPYVVGGLYNGRDVPNTGSVPLVDEASGTINVRRMETRRHH
                     SVDLVDKFGSEGIFVRTGDSKLAVELDVAATRITVTSQGEVSISGAKGVTVASPAGEI
                     ALQGNKVSIQGNEVAVEARMALTLKAGMATVIQGRPVKIN"
     gene            443..724
                     /locus_tag="VEV43_07895"
     CDS             443..724
                     /locus_tag="VEV43_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HYP23479.1"
                     /translation="MRPAARAGDATSHPGVLGGPGVPTVLIGGLPAATVGNPHTCAFP
                     PPAPPHPSTAVLRGSPTVLIGGRPAARVGDVVGCGAVIVSGAFDVLIGG"
     gene            726..1151
                     /locus_tag="VEV43_07900"
     CDS             726..1151
                     /locus_tag="VEV43_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HYP23480.1"
                     /translation="MSEEFVGTGWPFPLRADPNGAIALVSREREIEEAMRLILGTAYM
                     ERPMRPEFGCGIHDFVFVSADESALGRIAHEVRIALERWEPRVEVVHVVATIPEHRRD
                     TIDVEIGYSIKGSNDPRNLVFPFYVIPRDGETDAEGGPA"
     gene            1148..>1553
                     /locus_tag="VEV43_07905"
     CDS             1148..>1553
                     /locus_tag="VEV43_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HYP23481.1"
                     /translation="MTLPAPSLDDRRFQDLVDEAKRMVQQRCPEWTDHNVSDPGVTLI
                     ETFAFMVDQLLYRLNRVPDRLYVKLLDLVGVRRFPPTAATVDVTFRLSAPQPEPVVVP
                     AGTEVATPRTDVQEPVTFTVVEPLTIPPCSLDG"
BASE COUNT          233 a          551 c          545 g          224 t
ORIGIN      
        1 tgacttcggc cgcccctacg tcgtcggcgg cctctacaac ggcagggacg tgccgaacac
       61 gggatcggtc ccgctcgtag acgaagcgtc cgggacgatc aacgtgcgcc ggatggagac
      121 gcgcaggcac cactccgtcg acctcgtcga caagttcggc tccgagggga tcttcgtgcg
      181 gaccggtgac tcgaagctcg ccgtcgagct cgacgtcgcg gcgacgcgga tcaccgtgac
      241 ttcgcaggga gaggtgtcga tcagcggcgc gaagggcgtc acggtcgcga gccccgcggg
      301 tgagatcgcg ctgcagggca acaaggtctc gatccagggc aacgaggtcg ccgtcgaggc
      361 gcgcatggcg ctgacgctga aggccggcat ggcgacggtc atccagggac gtccggtcaa
      421 gatcaactga gaggaggagc acgtgcgtcc agccgcgcga gccggagacg cgacgagcca
      481 tcccggggtc ctgggggggc cgggggtgcc cacggtcctc atcggcggcc tgcccgcggc
      541 cacggtcggg aacccgcaca cgtgcgcgtt cccaccgccg gcgcctccgc atccctccac
      601 cgcggtccta cgcggcagcc cgaccgtcct gatcggcggg cgtcccgcgg cgcgcgtcgg
      661 cgacgtcgtc ggctgcggtg ccgtgatcgt ctccggcgcg ttcgacgtgc tgatcggcgg
      721 ctgacgtgag cgaggagttc gtcgggacgg ggtggccctt ccctctcagg gcggacccca
      781 acggcgcgat cgctctcgtg tcgcgcgagc gcgagatcga agaggcgatg cgcctgatcc
      841 tcgggaccgc ctacatggag cgccccatgc gcccggagtt cggatgcggg atacacgatt
      901 tcgtcttcgt gtcggccgac gagagcgcgc tcggccgcat cgcacacgag gtccgcatag
      961 cgctcgaacg ctgggagccg cgcgtcgagg tggtccacgt cgtcgcgacg atcccggagc
     1021 accgccgcga cacgatcgac gtcgagatcg gctactcgat caaggggtcg aacgacccgc
     1081 gcaacctcgt cttcccgttc tacgtgatcc ctcgcgacgg cgagacggac gcggagggag
     1141 ggccggcgtg acgctgcccg ctccctcgct cgacgaccgg cgcttccagg acctcgtgga
     1201 cgaggcgaag cggatggtcc agcagcgctg cccggaatgg accgaccaca acgtgtccga
     1261 cccgggcgtg acgctcatcg agacgttcgc gttcatggtc gaccagctcc tgtaccggct
     1321 caaccgcgtc cccgaccggc tctacgtcaa gctcctcgat ctcgtcgggg tgcgccgctt
     1381 cccgccgacg gccgcgaccg tcgacgtgac gttccggctg tcggcgcccc agcccgagcc
     1441 cgtcgtcgtc ccggcgggga ccgaggtcgc cactccgcgg accgacgtgc aggagcccgt
     1501 gacgttcacg gtcgtcgagc cgctcacgat cccaccgtgc tcgctcgacg gca
//
LOCUS       DASQDO010000282         7599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_886546, whole genome shotgun sequence.
ACCESSION   DASQDO010000282 DASQDO010000000
VERSION     DASQDO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886546"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VEV43_07910"
     CDS             <1..231
                     /locus_tag="VEV43_07910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP23482.1"
                     /translation="PVLVSDMGGLPELLSEGGGFSTPAGDTGALAEKIELLGADDELC
                     TELGAQAHEFARRHLTPERHVHAVDEAYRTLA"
     gene            complement(228..1097)
                     /locus_tag="VEV43_07915"
     CDS             complement(228..1097)
                     /locus_tag="VEV43_07915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP23483.1"
                     /translation="MTSSDIDVSVLTPSYGYGRYISNALDSAMGGRSLRIEHIVQDAC
                     SEDDTTTVLERARDRYGDAVKWAIEPDKGQSDALNKAFSVAQGRWIGWLNADEFYLPR
                     GLDELVAAGDRTGADVVYGDAVFVDAEGRLMRLKPQHRFNEFILRNYGVFISTCATIF
                     RRSALWPDPWDVHLRLLMDHDIYLKLAHKSAEFAYVPYPVAAFRVHSERVSAEPDAFG
                     GDYAIVQGRHGRGGTIAKGAAWLLHAVYKATSGGYRRQRQANSLVGEPMAWTDPGDDE
                     RGVERLLQTCYGR"
     gene            complement(1118..2302)
                     /locus_tag="VEV43_07920"
     CDS             complement(1118..2302)
                     /locus_tag="VEV43_07920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP23484.1"
                     /translation="MKILAFAYACEPGKGSEPGAGWMLVRNLATFGEVCVITRANNRE
                     AIEARSADDDDLAGVRFVYVDLPAWARSWKKGMRGMRLYYVLWQLAALRAARRLEASE
                     RFDCVWHLTIANVWLGSAAALAGPPFLYGPVGGGVRAPLGLVPSLGLRGAVYEALREV
                     ARQGSRYLNQLARVSWRNASVILVQNEETRRWLPTRHRRKAVVYQNAAVEGVDLESAV
                     GGPGRTAVFAARLIAWKGGALAIRAIARSPQWRLIVCGTGPDEDRLKRLAEKLRVGDR
                     VDFRGRVPQEQLLSLYATEADVFLFPSIHDDSPLAVAEAVAAGLPVVCLDVGGPHLIA
                     GDAGTVVSSRGRASKVVKALASALEEAAAGGPPDAATRRRVMMHDRKEGLLDIVTKAL
                     PR"
     gene            complement(2299..4500)
                     /locus_tag="VEV43_07925"
     CDS             complement(2299..4500)
                     /locus_tag="VEV43_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23485.1"
                     /translation="MKHEISEILALVGENVRVLHAPAGTAGAGTTGGGDIDCMVTGLD
                     PAWPLRLPPGWRLCQCLQYDVTGWSWIVEGEDDGPVAIDTLDDRAGLGKYGFPTSALR
                     ASGDGTGAGIDAAYLVAKRIYKRLFDPADWRAASELARSDPVSFERALHETFGPAVAE
                     ALGASVTRGVPPGEATARRARRAQFRRRVRTPARALSLLGMSAARLWRRVARPTGMYV
                     VLSGPDGTGKSSVADGLLRTTKPLFRRSYRAHWRPGILPRPGAVVGREERDATNPHAT
                     VARPAPVSVLFLLYYWLDYVLGSALSVLPRRIRTTFVLFERGWWDIAIDPLRYRLDVP
                     EWLVVALGRMLPRPDLIVTLEGDPSVLLARTQEISREEIVRQSRAWRDLSERVERVTF
                     VDASGSYEEVLRDVHLTITRWLEDRALRRLGHGWVSLPTREDPRWVLPRGPRRVAVRA
                     AALYQPVTRSGRLGWGLGRLLARAGGWRGLPRSAPPPRAALEALGPALVRGDTVASSR
                     SNHPGRFALMLVNERGIPDRVAKVVTDGPAAFLDREADVLERYAGFLPPPLAAPSVVA
                     RTPRAIVFASVDWKLRREPWRLAPDVARALGAFYRAAEGTEAGGPAHGDFAPWNLLQT
                     RTGWVLIDWEHFAEEDVPFYDLWHYFVQGHALLGRPSADEILRARSGVGDVGAALAAC
                     AEGARLPVDLAAASLEAYLSRTSAQMDLKREDGRAGLSARTTLLQRLRSDA"
     gene            4573..5640
                     /locus_tag="VEV43_07930"
     CDS             4573..5640
                     /locus_tag="VEV43_07930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP23486.1"
                     /translation="MKIALLYLDALRSGGYPRDVRWLASEFAGLGHEVVLIAREGEET
                     DGLRDVGVVRPERFRSLYGTVDLVHTWGLFLPAQIGVEWAGRRKAGAVVPTVVSPLAQ
                     LMPLHLGRSVWKKVPYLALLRPTLRGVNHVAHFFSEEEYWASVHYLQPTTHFEATLGL
                     FPPPGGATASDAVGDYLLFFGRNDITQKGIDVLVEGYRLARRQGLTLPLVVAGHPDKR
                     SVGYWKKVKRDLELSSHVELLGEVPDEERSRLLVDARCLVFPSRWDGPPRPIREAIAL
                     CTPVIVTNGTNMGHLVEAHSAGELVALEAKSISEGLLNAADEEKARAWREGTKKLRET
                     LAWDRVALEYLRGYEMTLAAV"
     gene            complement(5637..6749)
                     /locus_tag="VEV43_07935"
     CDS             complement(5637..6749)
                     /locus_tag="VEV43_07935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP23487.1"
                     /translation="MRLLVFAHALQGRGTERGLVRLLAELDRSVVTPELAIASARGEF
                     LASVPDDVVVHDLGIGDRRTSAALPALAKLVRSRGPDCAMGVHISAGRVVSALRLFRP
                     RLPVICMEADPFTRIEGDKGNLALRKTASRATYRLASHVVAASDVVADDLVTHLGVDR
                     RKITVIPLPSVDDAVFSLAREALDEPPFDSPDGPIVVTIGNMFAHKDQATLLRAFAGV
                     IAEIPAHLVLIGEGPMRDPLEELANELDIAERVRFAGFQDNPFKYLARSSVFVSPSEA
                     EGFDISQIEAMACGLPVVVTDAPRFKAVDDGRTGLLVPPRDPSALSDAVLRVLRSPEL
                     AARLGENARAAALEFTSAKIARRYEELIRTVVHGRS"
     gene            6798..>7599
                     /locus_tag="VEV43_07940"
     CDS             6798..>7599
                     /locus_tag="VEV43_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP23488.1"
                     /translation="MTTQTAPQSSAARTVHIVPKGGLFDLELAELWSYRQLLFFFVWR
                     DVNVRYKQTLIGSAWAVLQPFAAMILFTVFFGNLLDLSTEGIPGPVFYYSALLPWTYF
                     SLAVVTASNSVVGNQNLIHKIYFPRLLLPMSSVLPGVIDFAIAFVVLLVLMLVYGVGF
                     GLPFLLFPLFLFMAVLLALALGVWLSILNARFRDIRFVVPFLVQMLFFASPVVYSADQ
                     IPDEWRVLYGLNPLVGIIDGFRWSITGVGGAPGLSVLLSLAFVLVVLVG"
BASE COUNT         1143 a         2723 c         2621 g         1112 t
ORIGIN      
        1 ccggtcctcg tctcggacat gggcgggctg ccggagctgc tgtccgaggg cgggggcttc
       61 agcactcccg caggtgacac cggcgcgctg gcggaaaaga tcgagctgct gggcgcagac
      121 gacgagctgt gcacggagct aggcgcgcag gcccacgagt tcgcgcgccg gcacctgacg
      181 ccggaacgcc acgtgcacgc ggtcgacgag gcataccgga cgctggccta gcggccgtag
      241 caggtctgca gaagcctctc gacgccccgc tcgtcgtcac cggggtcggt ccacgccatc
      301 ggctcgccga cgagcgagtt ggcctgtctc tgccgccggt agccgcccga ggtcgccttg
      361 tacacggcat ggagcagcca ggcggccccc ttggcgatcg tcccgccgcg gccgtggcgg
      421 ccctggacta tggcgtagtc cccaccgaac gcgtcgggct cggcgctgac cctctccgag
      481 tgcacgcgga acgcggcgac cggatacggg acgtaggcga actccgccga cttgtgagcg
      541 agcttcagat agatgtcgtg gtccatcagc aggcggagat ggacgtccca cgggtcgggc
      601 cacaaggccg acctgcggaa gatcgtcgcg cacgtggaga tgaagacgcc gtagttgcgc
      661 aggatgaact cgttgaagcg atgctggggc ttcagccgca tgagccgccc ttcggcgtcg
      721 acgaagacgg cgtccccgta gacgacgtcg gcccccgtcc ggtcaccggc ggccacgagc
      781 tcgtccagcc ctctcggcag gtagaactcg tcggcgttca gccatccgat ccagcgcccc
      841 tgggcgacgc tgaaggcctt gttgagcgcg tccgactgtc ccttgtcggg ctcgatcgcc
      901 cacttgaccg cgtctccgta acggtcgcga gccctttcga ggaccgtcgt cgtgtcgtcc
      961 tcggagcagg cgtcctggac gatgtgctct atgcggagcg aacgcccgcc catcgcgctg
     1021 tccagagcgt tgctgatgta gcggccgtac ccgtacgacg gagtcaagac gctcacgtct
     1081 atgtcgctgg acgtcaccgg tcccgctgct cccctgttca tcgcggaagg gctttcgtca
     1141 cgatgtctaa cagaccctct ttacggtcgt gcatcatcac ccgccgccgc gtggccgcgt
     1201 ccggagggcc tcccgcggca gcttcctcca gcgcggacgc cagcgccttc acgaccttcg
     1261 acgcgcggcc cctgctgctc acgacggtcc cggcgtcccc cgcgatgagg tgcggtcccc
     1321 cgacgtcgag gcacaccacc ggcaggcccg cggccaccgc ctccgcgacg gcaagcggcg
     1381 aatcgtcgtg gatgctcggg aagaggaaga cgtcggcctc cgtcgcgtag agcgacaaga
     1441 gctgctcttg cgggacccgt ccgcggaagt cgacgcggtc accgacccgc agcttctcgg
     1501 ccagccgctt gagcctgtcc tcgtccggac cggtcccgca cacgatcaac ctccactgcg
     1561 gcgagcgagc gatcgcccgg atggcgaggg cgcctccctt ccaggcgatc aggcgcgcgg
     1621 caaagacggc ggtacgcccc ggccccccta cggccgactc gagatcgacg ccttcgaccg
     1681 cggcgttctg gtagacgacg gctttcctcc ggtgacgcgt gggcagccag cggcgcgtct
     1741 cttcgttctg gaccaggatc acggacgcgt tgcgccacga gacccgcgcg agctggttca
     1801 ggtacctcga cccctgccgc gccacttcac ggagcgcctc gtacaccgcg cccctgaggc
     1861 cgagcgacgg gacgaggccc agaggcgctc tcacgccgcc gccgacggga ccgtagagaa
     1921 acgggggccc ggcgagcgcg gccgccgagc cgagccacac gttggcgatc gtgagatgcc
     1981 acacgcagtc gaatcgttcg ctcgcctcga gacgccgggc cgcacgcagg gcggccagtt
     2041 gccagaggac gtagtagagg cgcatgccgc gcatgccctt cttccacgaa cgcgcccagg
     2101 ccgggaggtc gacgtagacg aagcgcacgc ccgcgaggtc gtcgtcgtcc gccgaccgcg
     2161 cctcgatcgc ctcacggttg ttggcgcggg ttatcacgca cacctcgccg aacgtcgcga
     2221 ggttccggac gagcatccac ccggcccccg gctccgatcc cttgccgggc tcgcaggcgt
     2281 acgcgaacgc caggatcttc aagcgtccga cctgagccgt tgcaacaacg tggtccgcgc
     2341 ggacagcccc gcgcgaccgt cctctctctt caagtccatc tgtgcggagg tccgtgacag
     2401 gtaggcctcg agcgacgccg cggccagatc gacggggagc cgcgctccct ccgcgcaagc
     2461 cgcgagcgca gcgccgacgt ctcctacgcc ggaccgtgca cgaaggatct cgtcggcgga
     2521 agggcggccg aggagagcgt gaccctgcac gaagtagtgc cacaggtcgt agaacgggac
     2581 gtcctcctcc gcgaagtgct cccagtcgat cagcacccat cccgtcctcg tctgcaggag
     2641 gttccacggg gcgaagtcgc cgtgcgccgg cccccccgcc tcggtgcctt cggccgcgcg
     2701 gtagaacgcg cccagagcgc gggcgacgtc cggcgcgagc cgccacggct cccggcgcag
     2761 cttccagtcg accgacgcga agacgatcgc tcggggcgtc cgcgcgacga ccgacggcgc
     2821 ggcgaggggc ggcggcagga accccgcgta cctctcgagc acgtccgcct ccctgtcgag
     2881 gaacgcggcc ggcccgtcgg tcacgacctt ggcgacacgg tcggggatgc cgcgctcgtt
     2941 gaccagcatc agcgcgaacc gcccgggatg gttggagcgg gacgaggcga ccgtgtcccc
     3001 gcggacgagg gccgggccca gggcctcgag ggcagcgcga ggagggggcg cggagcgggg
     3061 aaggccccgc cacccgccgg cccgcgcgag gaggcgcccc agcccccagc cgaggcgtcc
     3121 cgagcgcgtg acgggctggt acagcgcggc ggcgcgcacg gctacgcgcc gggggccgcg
     3181 gggcaacacc cagcgcgggt cctcgcgggt gggcagcgac acccagccgt ggccgagcct
     3241 ccgcagagcg cggtcctcca gccagcgcgt gatcgtgagg tggacgtcgc gcaggacctc
     3301 ttcgtaggac ccggacgcgt cgacgaacgt gactcgctcg acccgctcgg agaggtccct
     3361 ccacgcgcgc gactgccgga cgatctcttc acgagagatc tcctgcgtgc gcgccagcag
     3421 gacggacggg tcaccttcga gcgtgacgat caggtcgggc cgcgggagca tcctgccgag
     3481 cgcgacgacc agccactccg ggacgtcgag gcggtagcgc agcgggtcga tcgctatgtc
     3541 ccaccacccg cgctcgaaca ggacgaacgt ggtgcggatc cgccgcggaa ggacggacaa
     3601 cgccgacccg aggacgtagt cgagccagta gtacaagagg aacaggaccg agacgggggc
     3661 cggtcgcgcg acggtggcgt gcgggttcgt ggcgtcgcgt tcctctcgcc cgacgaccgc
     3721 ccccggccgc ggcaggattc cgggacgcca gtgcgcgcgg taggagcggc ggaacagggg
     3781 cttggtcgtt cgcaggaggc cgtcggccac cgacgacttg ccggtcccgt cggggccgct
     3841 caggaccacg tacatcccgg tcgggcgcgc gacgcggcgc cacaggcgcg ccgccgacat
     3901 gccgagcagg gacagggcac gcgccggcgt ccggacgcgg cgccggaatt gagcgcggcg
     3961 cgcgcggcgc gccgtcgcct cgccgggcgg aacgccgcga gtcacgctcg cgccgagagc
     4021 ctccgcaacc gcgggcccga acgtctcgtg cagagcgcgt tcgaaagaca cggggtccga
     4081 acgcgcgagc tcggacgccg cgcgccagtc cgccgggtcg aacagccgct tgtagatccg
     4141 tttggcgacc aggtaggccg cgtcgatccc ggcccccgtg ccgtcgcccg aggcacgcaa
     4201 cgccgacgtc gggaacccgt acttgcccag ccccgcgcgg tcgtcgaggg tgtcgatcgc
     4261 gacggggccg tcgtcttctc cttcgacgat ccacgaccac cccgtgacgt cgtactgcag
     4321 gcactggcac agccgccatc ccggcgggag acgcaggggc cacgccgggt cgagcccggt
     4381 gaccatgcag tcgatgtcgc cgccgcccgt cgtccccgct ccggccgtgc ccgcgggcgc
     4441 gtgcaggaca cgcacattct cgcccacgag agcgaggatc tccgagatct cgtgcttcat
     4501 cgagggtgaa ggttacggga gggcgaatgc tcgcactact atcgcgaaag tgctgccggc
     4561 cgcccccacg gtatgaagat cgccctcctc tatctggacg cgctccggtc cggggggtac
     4621 ccacgcgacg tccgctggct cgcctccgag ttcgccggcc tcggacacga agtcgtcctc
     4681 atcgcccgcg agggggagga gaccgacggg ctgcgcgacg tcggggtcgt ccggcccgaa
     4741 cgattccgct ccctgtacgg gaccgtcgat ctcgtccaca cctggggcct cttcctgccc
     4801 gcgcagatcg gcgtcgaatg ggccggccgg cgcaaggccg gcgccgtcgt ccccacggtc
     4861 gtctcgcccc tcgcgcagct gatgccgttg cacctcgggc gttccgtgtg gaagaaggtc
     4921 ccctacctgg cgctgctgag gccgacgctc aggggcgtca accacgtcgc gcacttcttc
     4981 tccgaagagg agtactgggc gtcggtgcac tacctgcaac cgacgacgca cttcgaagcg
     5041 acgctgggcc tcttcccgcc gcccggtggc gcgaccgcgt ccgacgcggt cggcgactac
     5101 ctgttgttct tcgggaggaa cgacatcacc cagaagggca tcgacgtgct cgtggagggc
     5161 taccgcctgg cgaggcggca ggggctgacg ctgccgctgg tggtcgccgg ccaccccgac
     5221 aagaggagcg tcgggtactg gaagaaggtc aagagggacc tggagctgtc cagccacgtc
     5281 gagctgctgg gcgaggtccc cgacgaagag cggtcgcggc tgctcgtcga cgcgcgctgc
     5341 ctcgtcttcc ccagccgctg ggacggccct ccgcggccga tccgcgaagc gatcgccctg
     5401 tgcacgccgg tgatcgtcac gaacggcacc aacatgggcc acctcgtgga agcccattcg
     5461 gccggcgagc tcgtggcgct cgaggccaag tcgatcagcg aagggctcct gaacgcggcg
     5521 gacgaggaga aggcgcgggc ctggcgggag gggacgaaga agctacgcga gaccctggcg
     5581 tgggatcgcg tcgcgctcga gtacctccgc ggctacgaga tgacgctcgc cgccgtctag
     5641 gagcgtccgt gcaccacggt gcggatcagc tcctcgtaac gtctcgcgat cttggcgctg
     5701 gtgaactcca gcgcggcggc gcgcgcgttc tcgcccagcc gggccgcgag ctccggcgaa
     5761 cggaggaccc gcaggacggc atccgacaga gccgagggat cgcgcggcgg caccagcaac
     5821 cccgtgcggc cgtcgtcgac ggccttgaag cgcggggcgt cggtgacgac taccggaagg
     5881 ccacacgcca tcgcctcgat ctgcgagatg tcgaacccct cggcctccga cggcgacacg
     5941 aagacggacg agcgcgcgag gtacttgaac gggttgtcct ggaagccggc gaaccgcacg
     6001 cgctccgcga tgtcgagctc gttcgccagc tcctcgagcg ggtcgcgcat cgggccctct
     6061 ccaatcagga cgagatgggc cgggatctcg gcgatcactc cggcgaacgc acgaagcaac
     6121 gtggcctggt ccttgtgggc gaacatgttc ccgatggtca cgacgatggg cccgtccggg
     6181 ctgtcgaacg ggggctcgtc cagggcttcg cgtgccagcg agaagaccgc gtcgtcgacg
     6241 gacggcagcg ggatgaccgt gatcttgcgg cggtcgacgc cgaggtgcgt cacgaggtcg
     6301 tcggcgacga cgtccgacgc cgccacgacg tggctcgcga gccggtacgt ggcgcgcgac
     6361 gcggtcttcc gcagcgcgag gttccccttg tctccttcga tccgcgtgaa cgggtcggcc
     6421 tccatgcaga tcaccgggag ccgcggacgg aacagccgca gcgccgacac gacccggccg
     6481 gccgagatgt gcacgcccat cgcgcagtcg ggaccgcgcg agcggacgag cttcgcgaga
     6541 gcgggcagcg cagccgaggt ccggcggtcc ccgatcccga gatcgtgcac gaccacgtcg
     6601 tccggcaccg acgcgaggaa ctccccgcgt gcggacgcga tcgcgagctc cggtgtgacg
     6661 accgaccgat ccagctcggc gagcaggcgc acgagccctc tctccgtccc ccggccctgg
     6721 agagcgtgag cgaaaacgag gagcctcacg ggatcactgg gcacgtcggt agtatccaac
     6781 gctaaaagga tggtgacttg accactcaga cggcccccca gtcgtcagcg gcgcgcacgg
     6841 tccacatcgt cccgaagggt ggcctgttcg acctcgagct ggcggagctc tggtcgtacc
     6901 ggcagctgct gttcttcttc gtctggcgcg acgtcaatgt ccgctacaag cagacgctga
     6961 tcgggtccgc gtgggccgtg ctccagccct tcgcggcgat gatcctgttc acggtcttct
     7021 tcgggaacct gctcgacctc tccaccgaag ggatccccgg cccggtcttc tattacagcg
     7081 cgctgctccc gtggacctac ttctcgctcg cggtcgtcac cgcgagcaac tcggtcgtcg
     7141 ggaaccagaa cctcatccac aagatctatt tcccgcggct gctcctgcct atgtcgtccg
     7201 tcctgccggg cgtgatcgac ttcgccatcg cgttcgtcgt cttactggtc ctgatgctcg
     7261 tctacggcgt cgggttcggc cttcccttcc tcctgttccc gctgttcctc ttcatggccg
     7321 tcctgctcgc gctcgcgttg ggggtctggc tctcgatact caacgcgcgc ttccgggaca
     7381 tccgcttcgt cgtcccgttc ctcgtgcaga tgctcttctt cgcgtcgccg gtcgtctact
     7441 cggccgacca gatcccggac gagtggaggg tgctctacgg gctcaacccg ctcgtcggca
     7501 tcatcgacgg gttccggtgg tcgatcaccg gggtgggagg ggcgccgggt ctctcggtcc
     7561 tgctgtcgct cgcgttcgtc ctcgtcgtcc tcgtcggcg
//
LOCUS       DASQDO010000283         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_275082, whole genome shotgun sequence.
ACCESSION   DASQDO010000283 DASQDO010000000
VERSION     DASQDO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275082"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VEV43_07945"
     CDS             <1..1409
                     /locus_tag="VEV43_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYP23489.1"
                     /translation="ADVDESMLTGVSRPVAKSAGDRVTAGTVATDSAIRVRVDAVGDE
                     TALAGIQRLVQEAQGSRSRAQALADRAAAFLFYAATLAGLVTYVAWTLFGEQRTAVER
                     TVTVLVIACPHALGLAIPLVVSISTALSARNGILVKDRLALERMRTVDVVLFDKTGTL
                     TAAQHRVTGVAAADGDAGGALALAGAVESDSEHPLARAIVRAAEERGTLPRATEFRSM
                     TARGVEARVDGRTLAVGGPKLLEDMGADVPGDLQDEVTAWKERGASVLFLLEGGKVHA
                     AFALEDAVRAESHEAVASLHARGVKVAMITGDAREVAASVAAELGVDEYFAEVLPEDK
                     DSKVAELQARGHRVALVGDGVTDAPALARADVGIAIGAGTDVAIESAGVILASDDPRS
                     VTGVVELSKASFRKMVQNLVWATGYNAFAIPLAAGVLSWAGVTLSPALGAVLMSVSTI
                     VVAINAQLLRRVDLRPGR"
     gene            complement(1406..2620)
                     /locus_tag="VEV43_07950"
     CDS             complement(1406..2620)
                     /locus_tag="VEV43_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYP23490.1"
                     /translation="MAGVGPDEVAAVFRAESARAVASLVRFFGDFDVAEEAVQDAIEV
                     ALQRWTDGGLPPSPAGWIITTARNRGIDRLRREASRDDRHRQAALLHAESQPTEVDPV
                     GDDRLRLIFTCCHPALAPHAQVALTLRLIAGLQTHEIARAFLVADETMAQRLVRAKNK
                     IRAARIPYRIPEDAELPNRLRPVLAVVYLIFNEGYVAAAGDRLVREDLCAEAIRLGRL
                     LVELMPDEPEAKGLLALMLLTEARRPARVAPDGSLVRLADQDRAKWDHDLVREGHGLV
                     RACLRRNAPGPYQVQAAIAAVHADAPSAEETDWSQIVRLYDLLLELAPTPVVALNRAV
                     AVAEAEGAAAGLAIVDELDLDGYHLFHSTRAELLRRLDRRDEAAAAYRRALDATENAT
                     ERAHLERRLASL"
     gene            complement(2629..2970)
                     /locus_tag="VEV43_07955"
     CDS             complement(2629..2970)
                     /locus_tag="VEV43_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYP23491.1"
                     /translation="MTQYLMAVHHDFDAPLPADMEQIYADVDAFNKKLTDAGAWVFGG
                     GLEAPSTATVLRAEGGEILTTDGPFAEVKEHLGGFWVIEAPDLDAALEWGSQAAVACR
                     NPIEVRPFQAE"
     gene            complement(3078..3500)
                     /locus_tag="VEV43_07960"
     CDS             complement(3078..3500)
                     /locus_tag="VEV43_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23492.1"
                     /translation="MGLSDRAAAFFDSYLKAFEAFDPEAIAAHFAYPFHMTSDGDEVG
                     LTAVPDEATWRAEIGRLVSFYGDVGVASARMLDASSTELSPRVEHAAIHWQLQDSAGT
                     DLYDFHGVYTLAEVAGETRIVALAHDEIPRALAFATRG"
     gene            3499..3648
                     /locus_tag="VEV43_07965"
     CDS             3499..3648
                     /locus_tag="VEV43_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23493.1"
                     /translation="MPPPYGDYGSRHDERRRRLLRAAPPPAERAGIALKGKAEPARLF
                     AVAAR"
     gene            3695..4177
                     /locus_tag="VEV43_07970"
     CDS             3695..4177
                     /locus_tag="VEV43_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23494.1"
                     /translation="MAGSTGRPDPELFRPTVTGTPAYDPAPWRPDSNFYVAFFGGIVP
                     ATIIAFLNALRLHAPEAARRVLVAGVAALALFALGAYLIGTNVEDAEAARRYSRLGVR
                     AGGVLVHFLFLSWLKGPWRRFEIVRGTEPAPMWGPGFGAWLVGILVQAAVTVALMEQV
                     "
     gene            4174..5061
                     /locus_tag="VEV43_07975"
     CDS             4174..5061
                     /locus_tag="VEV43_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYP23495.1"
                     /translation="MSETFERLDRSLAQARHWLSMGRPEKVLDALADAGPAATTSPDA
                     FLLRAAAHTQLRRWEEARQAAEDGLALDPRDHRLLEVLGIVHWNRGDRPAAEKAFLAG
                     LQSVPEDAHLLARYAMLVAEDGQLDKAGKLASKAAAVRPQDPLVAEVRSKIAYLAGDT
                     KAAREISAAGLAHAPEDPHLHALTGSYLLEDGSIGRATKHHLTSASIDPGDENLRDLG
                     RQAKYLRNPLLRPMWFIEKVGPGKIWLAWIAIFFGLRAAGLVTIAAMLSLCYLVVVVY
                     SWVVPPLLRRYYKLGKDDL"
     gene            5058..>5618
                     /locus_tag="VEV43_07980"
     CDS             5058..>5618
                     /locus_tag="VEV43_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23496.1"
                     /translation="MNDRIDSLRRAVEASPDDTTLRELLAEELVREGYVTEALNEFET
                     LLARGQLSDASALWAGHVAVDAERADLAKGFLEAARTAGVVEGLAALQARVDGLVAQR
                     APMVRADDVPDGPASPWEKEVVSTTFDRVGGLDDVKKAINRAIVLPFSRPDLYERYGR
                     KSGGGILLYGPPGCGKTLLARATAGEC"
BASE COUNT          771 a         2019 c         2030 g          798 t
ORIGIN      
        1 gggcggacgt cgacgagtcg atgctcaccg gcgtgtcgcg gccggtcgcg aagtctgcag
       61 gcgaccgcgt cacggcgggg acggtggcta ccgactccgc gatccgggtg cgcgtcgacg
      121 cggtcggcga cgagaccgcg ctcgcaggca tccagcggct cgtgcaggag gcgcagggat
      181 cgcgctcgag ggcccaggcc ctcgcggacc gcgcggccgc gttcctcttc tacgccgcga
      241 cgcttgcggg gctcgtcacg tacgtagcgt ggacgctctt cggcgagcag cgcactgcgg
      301 tcgagcgaac cgtgaccgtg ctcgtgatcg cgtgccctca cgctctcggc ctggcgatcc
      361 cgctcgtggt ctcgatctcg acggcgctgt cggcccgcaa cgggatcctc gtcaaggacc
      421 gcctcgcgct cgagcggatg cgtaccgtcg acgtagtgct gttcgacaag accggcacgc
      481 tgaccgccgc gcagcaccgc gtgaccggcg tcgccgcggc agacggagac gccggcggag
      541 ccctcgcgct cgccggagcg gtcgagtccg acagcgagca tccgctcgcc cgtgcgatcg
      601 tccgcgcagc ggaagaacgc ggcacgctcc cccgcgccac ggaattccgt tcgatgaccg
      661 cccgcggcgt cgaggcgagg gtcgacggcc ggacgctcgc ggtcggaggg ccgaagctgc
      721 tcgaggacat gggggccgac gtccccggcg acctgcaaga cgaggtcacc gcgtggaagg
      781 agcgcggggc atcggtcctg ttcctgctcg aaggaggcaa ggtccacgcc gcgttcgcgc
      841 tcgaggacgc cgtgcgcgcc gagtcgcacg aggcggtcgc gagcctgcac gcacgaggcg
      901 tcaaggtggc gatgatcacc ggcgacgccc gcgaggtcgc cgcgtcggtg gcggccgagc
      961 tcggcgtcga cgagtacttc gccgaggtgc ttccggagga caaggactcg aaggtcgccg
     1021 agctccaggc acgcggtcac cgcgtcgcgc tggtcggcga cggcgtgacc gacgcgccgg
     1081 cgctggcccg agccgacgtg ggcatcgcga tcggcgcggg gaccgacgtc gcgatcgagt
     1141 ccgcgggcgt gatcctggcg tccgacgacc cccgttctgt gaccggcgtc gtcgagctct
     1201 cgaaggcgtc gttccgcaag atggtccaga acctcgtgtg ggcgacgggc tacaacgcgt
     1261 tcgcgatccc gctggccgcc ggcgtcttgt cctgggccgg agtcacgctc tcccccgcgc
     1321 tcggagcggt gctgatgtcc gtgtccacga tcgtcgtggc gatcaacgcg cagctgctgc
     1381 gacgggtcga cctgcgtccc ggccgctaga ggctcgcgag ccgccgttcg aggtgggcgc
     1441 gctcggttgc gttctcggtc gcgtcgagcg cacgccggta agcggcagcc gcttcgtcac
     1501 ggcggtcgag acgccgcagc aactcggccc gcgtcgagtg gaagaggtgg tagccgtcga
     1561 ggtcgagctc gtcgacgatc gcgagcccgg ccgcggcccc ctcggcctcc gcgactgcga
     1621 ccgcccggtt cagcgcgacc accggcgtcg gcgccagctc gagcagcagg tcgtagagcc
     1681 gcacgatctg cgaccaatcg gtctcctcgg cggacggcgc gtccgcgtgg acggcggcga
     1741 tcgcggcctg cacctggtac gggcccggcg cgttgcggcg caggcaggcg cgcacgagcc
     1801 cgtgtccctc gcgcacgagg tcgtggtccc acttcgcccg gtcctggtcg gcgagccgca
     1861 cgagcgaccc gtccggtgcg acccgtgcgg gccggcgcgc ctcggtcagc agcatcagcg
     1921 cgagcagccc cttcgcctcc ggctcgtcgg gcatcagctc gacgagcagg cgcccgagcc
     1981 ggatcgcctc ggcgcacagg tcctcgcgca ccaaacggtc ccccgccgcc gcgacgtagc
     2041 cctcgttgaa gatcaggtag acgaccgcga ggaccgggcg cagccggttc ggcagctccg
     2101 cgtcttcggg gatccggtac gggatgcgcg cggcccggat cttgttcttc gcacgcacca
     2161 gccgctgcgc catggtctcg tccgcgacta ggaaagcgcg tgcgatctcg tgcgtctgta
     2221 gccccgcgat cagtcggagc gtgagcgcga cctgcgcgtg cggtgcgagc gcggggtgac
     2281 agcacgtgaa gatcagccgg agacggtcgt cgcccacggg gtccacctcc gtcggttggc
     2341 tctccgcgtg gagcaacgcg gcctgccggt gccggtcgtc gcgcgacgcc tcgcggcgaa
     2401 gccggtcgat gccgcggttg cgcgccgtcg tgatgatcca gccggccggg ctcggtggga
     2461 ggccgccgtc cgtccaccgc tggagcgcga cctcgatcgc gtcctggacc gcctcctcgg
     2521 cgacgtcgaa atcaccgaag aagcggacca ggctcgcaac cgctctggcg gactcggcac
     2581 ggaagaccgc cgctacctcg tcggggccga cccctgccat ccggcccgct actcggcctg
     2641 gaacggacgg acctcgatcg ggttgcggca cgcgaccgcc gcctgcgacc cccactcgag
     2701 cgccgcgtcg aggtcggggg cctcgatgac ccagaagccg ccgaggtgct ccttgacctc
     2761 cgcgaaggga ccgtcggtcg tgaggatctc gccgccctcg gcccgcagga ccgtcgcggt
     2821 cgacggcgcc tcgaggcctc cgccgaagac ccacgccccg gcatccgtga gcttcttgtt
     2881 gaaggcgtcg acgtcggcgt agatctgctc catgtccgcc gggagcgggg cgtcgaagtc
     2941 gtgatgcact gccatcaggt actgcgtcat ggtggtcctc ctgctctcgg tgccggggct
     3001 tctccccgtt ctcgtgcccg acacgaacgg cggcggccgg aatcgacacg ccgccggaaa
     3061 aaagctcgaa gaattgttca gccgcgggtg gcgaaggcca gcgcccgggg gatctcgtcg
     3121 tgcgcgagcg ccacgatccg cgtctccccg gccacctcgg cgagcgtgta cacgccgtgg
     3181 aagtcgtaca ggtcggtacc ggccgagtcc tggagctgcc agtggatcgc ggcgtgctcg
     3241 acccggggcg agagctcggt cgaggacgcg tccagcatcc gcgccgaggc gacgccgacg
     3301 tccccgtaga acgagacgag acggccgatc tcggcccgcc acgtggcctc gtcgggaacc
     3361 gccgtcagcc cgacctcgtc gccgtcgctg gtcatgtgga aggggtaggc gaagtgcgcc
     3421 gcgatcgcct ccgggtcgaa tgcctcgaac gccttcagat acgagtcgaa gaacgccgcg
     3481 gcgcggtccg agagccccat gccgccaccc tacggcgact atggttctcg gcatgacgag
     3541 cggcggcgcc ggctgctgcg tgcggctccc ccgccggccg agcgggccgg gatcgcgctg
     3601 aagggcaagg ccgagccggc acggctgttc gccgtcgccg ctcggtaaga ttccgcccgt
     3661 tcacaggcct agtagtggga gtgggaggcg cgagatggcg ggctcaacgg ggcggccgga
     3721 ccccgagctg ttccggccga ccgtcaccgg caccccggcc tacgaccccg cgccgtggag
     3781 gcccgactcg aacttctacg tcgcgttctt cggcgggatc gtgccggcga cgatcatcgc
     3841 cttcctcaac gcgctcaggc tgcacgcccc cgaggccgca cgccgcgtcc tcgtcgccgg
     3901 cgtcgccgcc ctcgcgctgt tcgccctcgg cgcctacctg atcggcacca acgtcgaaga
     3961 cgcggaggcg gcgcgccggt actcccggct cggagtgcgg gccggaggcg tcctcgtcca
     4021 cttcctcttc ctctcgtggc tgaagggccc gtggcgccgg ttcgagatcg tccgcggcac
     4081 cgagcccgcg ccgatgtggg ggccggggtt cggcgcgtgg ctggtaggca tcctcgtgca
     4141 ggccgcggtc accgtcgcct tgatggagca ggtgtgagcg agaccttcga acggctcgac
     4201 cgcagcctgg cgcaggcgcg ccactggctc tcgatgggac gccccgagaa ggtgctcgac
     4261 gcgctcgcgg acgcggggcc cgcagcgacc acgagtcccg acgccttcct gctccgggcc
     4321 gccgcccaca cccagctccg gcgatgggag gaggcgcggc aggcggccga ggacgggctc
     4381 gccctggacc ctcgcgacca ccgcctcctc gaggtgctcg ggatcgtcca ctggaatcga
     4441 ggtgaccggc cggcggccga gaaggcgttc ctcgcgggtc tgcagagcgt gcccgaggac
     4501 gctcatcttc tagcgcgcta cgccatgcta gtcgccgagg acggccagct cgacaaggcg
     4561 ggcaagctcg cgtcgaaggc cgccgcggtc aggccgcagg acccgctcgt cgccgaggtc
     4621 cggtcgaaga tcgcgtacct ggccggcgac acgaaggcgg cgcgggagat cagcgcggcg
     4681 ggcctcgccc acgcgcccga ggaccctcat ttgcacgcgc tgacgggctc gtacctcctg
     4741 gaagacggtt cgatcgggcg cgccacgaaa caccatctga cgtccgcgtc gatcgacccc
     4801 ggcgacgaga acctgcgcga cctcggccgg caggcgaagt acctgaggaa cccgctgctg
     4861 cgaccgatgt ggttcatcga gaaggtcggc ccgggcaaga tctggctcgc ctggatcgcc
     4921 atcttcttcg ggctccgggc agccggcctg gtgaccatcg ccgccatgct ctcgctgtgc
     4981 tacctcgtcg tcgtcgttta cagctgggtc gtgcccccgc tgctccggcg ctattacaag
     5041 ctcggcaagg acgacctgtg aacgaccgca tcgattcgct ccgccgcgcg gtcgaggcgt
     5101 cgcccgacga cacgacgctg cgagagctcc tcgccgaaga gctcgtgcgc gagggctacg
     5161 tgaccgaggc cctcaacgag ttcgagaccc tgctcgcccg ggggcagctc tccgacgcgt
     5221 cggcgttgtg ggccggccac gtcgcggtgg acgcggagcg cgccgacctc gccaaggggt
     5281 tcctcgaggc cgcgcgcacg gctggagtag tcgaggggct cgcggcgctc caggcacggg
     5341 tcgacgggct cgtggctcag cgcgcgccga tggtgcgtgc cgacgacgtg ccggacggcc
     5401 cagcctcccc gtgggagaag gaggtcgtct cgacgacgtt cgaccgggtc ggcgggctcg
     5461 acgacgtgaa gaaggcgatc aaccgcgcga tcgtgctgcc gttctcgcgt cccgatctct
     5521 acgagcgcta cgggcgcaag agcgggggcg ggatccttct ctacggcccc cccgggtgcg
     5581 gcaagacgct gctcgcgcgg gccacggccg gagagtgc
//
LOCUS       DASQDO010000284         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_213692, whole genome shotgun sequence.
ACCESSION   DASQDO010000284 DASQDO010000000
VERSION     DASQDO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213692"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2505)
                     /locus_tag="VEV43_07985"
     CDS             complement(<1..2505)
                     /locus_tag="VEV43_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HYP23497.1"
                     /translation="METDAAPATIRTPDQRLRVFVSSTLGELADERKAARRAVEQLRL
                     APIMFELGARPHPPRALYRSYLEQSDVFIGIYWQRYGWVAPDMDVSGLEDEYLLAQGM
                     PQLVYVKRPAPDMEPRLRDMLARLQEEDATSYKPFSDAAELERLLVDDLAILLTERFD
                     RSQRPSAPAAVSTNLPAPASDFVGRGDVLETLGSLLAADDVRLVTLTGAGGVGKTRLA
                     IESARAQTERFEDGVFFVDLAEQRTPDGAYAAIVHSVPMDRVTEDPPLQCLKGALRDR
                     HVLLVLDNFEQVLPAAAGIAQLLESCPRLHVLATSRAALNIRGERRFDVPPLSLPPDA
                     PASARAALEYEAVRLFRDRAAAVRPDFEVTDANAADVAAICAQLDALPLAIELAAARL
                     NLFAVDELRTRLEKHVPVLGGAAHDVPRRQQTLRDTIEWSYDLLKEDERTLFQLCSVF
                     SDASLTDVEATAARVESLTVDVVDALARLVDKSLVRTVTGAEGRPRFSMLQTIRDFAT
                     EQLDARQELADVIRRAHAEHYAEVADRLRAAARAQGRDAVLASLSSELGNLRTAWSFW
                     GEHADVDRLDALLEPLWGYYDARGNYRAAMELAEELLRVLAGQPETPERIRDQLAVET
                     ALARSLIAARGFSSESERKIAEIFGRAESAGADHRFPALRTLAWFHLLRAEFPETAEV
                     AVRLLALADKEGDAAFQSEAHLVFGVTRLWVEDMPAALHHVDEALRYAEGARPGLVRL
                     RVGPDPRVLSLVVSGLIKWSGGYPEQALARAASGVELAREIDHPYSLAYALFHAGLLD
                     LWSADLGVLTERANELLEVATKHDYPIWRALAHVLHG"
     gene            complement(2716..3111)
                     /locus_tag="VEV43_07990"
     CDS             complement(2716..3111)
                     /locus_tag="VEV43_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020170612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP23498.1"
                     /translation="MAEFPALGHVAVTVTDIDRGREWYSRLFGSEPALDEDAGSFYHV
                     VWALGGGTLFGIHTHPRENDQPDFSEFRTGLDHVAFAVSSRAELESWATRLNELGIQH
                     GGIVDAPYGSGLSFRDPDNLPLEFFAPPS"
     gene            complement(3075..4025)
                     /locus_tag="VEV43_07995"
     CDS             complement(3075..4025)
                     /locus_tag="VEV43_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HYP23499.1"
                     /translation="MPVFETVKVGAIQACPVILDADATIDKALGLLHRAADEGVQLAV
                     FPETFVSVYPSGAWGHRASRFGGFDEMWERLWASSVEVPGPAVDKLLAACAERGIHCA
                     IGVNERELERPGSLYNTLLLMGPGGLLAKHRKLVPTMHERLFHGTGDGTDLDVVETEL
                     GRVGGLICWENRMPLARYELYGKGVQIWVAPTADDSDGWLASMRHIALESGAFVVSAP
                     QYIDRDAFPADFPVRLPDDEVFGRGGAAIVEPRGGEVVAGPLYGEEGMVTAEIDLKVG
                     LHAKRWFDVVGHYKRHDSGISRPDPGGAGWQSSPLSDTSQ"
     gene            complement(4065..>4246)
                     /locus_tag="VEV43_08000"
     CDS             complement(4065..>4246)
                     /locus_tag="VEV43_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HYP23500.1"
                     /translation="STRSHAELDEAEDLDEGDPADLARRYAELRTSLPGLNVLGGCCG
                     TDHRHVAAIRDACAG"
BASE COUNT          655 a         1484 c         1489 g          618 t
ORIGIN      
        1 gccgtgcagc acgtgggcaa gcgctcgcca gatcgggtag tcgtgtttgg tcgccacttc
       61 caggagctcg ttcgcgcgtt ccgtcaggac acccaggtcc gcggaccaca ggtccaggag
      121 gcccgcgtgg aacagggcgt acgcgagcga gtacggatgg tcgatctccc tcgcgagctc
      181 cacgccgctc gccgcccggg caagcgcctg ctccgggtag ccgccggacc acttgatcag
      241 tcccgagacc acgagcgaca ggaccctcgg gtcgggcccc acgcgcaacc ggacgaggcc
      301 ggggcgcgcg ccctccgcgt atcgcagggc ttcgtcgacg tgatggagcg ctgccggcat
      361 gtcctccacc cacagcctcg tcacgccgaa caccaggtgc gcctcggact ggaacgccgc
      421 gtccccttcc ttgtcggcga gcgcaagcag gcgcaccgcg acttcggcag tctcgggaaa
      481 ctccgcacgc aacaggtgga accaggcgag agtgcggagc gcggggaagc gatggtcggc
      541 ccccgcagac tcagctcggc cgaagatctc ggcgatcttg cgctcggact cggaggagaa
      601 tccccgtgcc gcgatcagcg atcgcgccag cgcggtctcg accgcgagct ggtcccggat
      661 ccgctcgggc gtctcgggct ggccggccag gacacggagc agctcctctg cgagctccat
      721 cgccgcgcgg tagttcccgc gggcgtcgta gtaaccccac agcggctcga gcagggcgtc
      781 caggcgatcc acgtcggcgt gctcgcccca gaacgaccac gcggttcgca ggttgccgag
      841 ctcgctcgaa agagacgcga ggacggcgtc gcggccctgc gcgcgtgccg cggcgcgcaa
      901 tcggtccgcg acctccgcgt agtgctccgc gtgagcgcgc cggatcacgt ccgcgagctc
      961 ctgccgggcg tcgagctgct ccgtcgcgaa gtcgcggatc gtctggagca tcgagaaccg
     1021 gggtcgtcct tcggcccccg tgacggtgcg gacgaggctc ttgtcgacga ggcgcgccag
     1081 cgcgtcgacg acgtcgacgg tcagcgactc gacgcgggcc gcggtcgcct cgacgtccgt
     1141 gagcgaagca tcggagaaca ccgagcacag ctggaacagc gtccgctcgt cctccttcag
     1201 gaggtcgtag ctccactcga tcgtgtcgcg caacgtctgc tggcgtcggg gcacgtcgtg
     1261 ggcggcgccg ccgaggaccg ggacgtgctt ctcgagccgc gtgcgcagct cgtcgaccgc
     1321 gaagaggttc aggcgcgccg ccgcgagctc gatcgcgagc ggaagggcgt cgagctgcgc
     1381 gcagatggcc gcgacgtcgg ccgcgttggc gtcggtgacc tcgaagtcgg gtcgcaccgc
     1441 ggccgcgcgg tcgcggaaga gccggaccgc ctcgtactcg agcgcggccc tcgccgacgc
     1501 gggggcgtcg ggcggcagcg acaacggtgg gacgtcgaac ctgcgttcac cccggatgtt
     1561 gagcgccgcc cggctggtcg ccagcacgtg gagccgcggg cagctctcga gaagctgcgc
     1621 gatccctgcc gctgcgggca gcacctgctc gaagttgtcg agcaccaaga gcacgtggcg
     1681 gtcgcgcaag gcgcccttca ggcattggag gggcggatcc tcggtgacgc ggtccatcgg
     1741 gacggagtga acaatggcgg cgtaggcgcc gtcgggcgta cgttgctccg cgaggtcgac
     1801 gaagaacacg ccgtcttcga atcgttccgt ctgcgcccgc gcggactcga tggcgaggcg
     1861 cgtcttgcct actcctccgg cccccgtgag cgtgacgagt cgaacgtcgt cggccgcgag
     1921 caggctcccc aacgtctcca gcacgtctcc ccttcccacg aagtcggagg cgggcgccgg
     1981 caggttcgtc gagaccgccg ccggcgcgct gggtcgctgg ctgcggtcga aacgctccgt
     2041 caggaggatc gctaggtcgt cgacgagcag cctctcgagc tccgcggcgt ccgagaacgg
     2101 cttgtacgag gtcgcatcct cctcttggag ccgcgcgagc atgtcccgta gccgcggctc
     2161 catgtcgggg gccgggcgct tcacgtagac gagctgcggc atcccctgcg cgaggaggta
     2221 ctcgtcttcg agccccgaga cgtccatgtc gggcgcgacc cagccgtagc gctgccagta
     2281 gatcccgatg aacacgtcgg actgctccag atacgagcgg tagagcgcgc gcggggggtg
     2341 cggacgggcg ccgagctcga acatgatcgg cgccagacgc agctgctcga ccgccctgcg
     2401 cgcggccttc cgttcgtcgg cgagctcgcc tagggtcgag gagacgaaga ctcgaaggcg
     2461 ctggtcgggc gtacggatcg tcgccggggc tgcatccgtc tccatccgcc agaccctacg
     2521 cccgggcgtt acccggtcac atcgctacac gcaccgacga acgccacggc gaccaggccc
     2581 gcggcccacg cccggagaat cctcttcgtg cccacggggg cgaaggctga aggggaaggg
     2641 gccgggccgg cggatccgcg tgccgccgag cccgcgtcta ggagtcggcg agctccaggg
     2701 cgtagccgcg gatcactagg agggcggggc gaagaactcc agaggcaggt tgtcggggtc
     2761 gcggaaggac aagcccgacc cgtagggggc gtcgactatc ccaccgtgct ggatgcccag
     2821 ctcgttcaga cgcgtcgccc aggactcgag ctccgcacgg ctcgatacgg cgaacgccac
     2881 gtggtccagg cccgttcgga actcgctgaa gtcgggttgg tcgttctcgc gcggatgcgt
     2941 gtggataccg aacaaggtcc ccccgccgag cgcccagacc acgtgataga agctcccggc
     3001 gtcttcgtcc agcgccggct ccgacccgaa caaccgcgag taccactcgc gcccgcgatc
     3061 gatgtccgtg acggttactg cgacgtgtcc gagagcgggg aactctgcca tccagcacct
     3121 cctgggtccg gtcgagagat acctgagtcg tggcgcttgt agtggccgac tacgtcgaac
     3181 cacctcttcg cgtgcagtcc caccttcagg tcgatctccg ccgtgaccat gccctcctcg
     3241 ccgtagaggg ggccggcgac gacctcacca ccacgcggct cgacgatcgc cgctcccccg
     3301 cggccgaaga cctcgtcgtc gggcaggcga accgggaagt cggcggggaa agcgtcccga
     3361 tctatgtatt gcggagcgct gacgacgaac gcacccgact cgagtgcgat gtgacgcatg
     3421 ctcgccagcc atccgtccga gtcgtctgcc gtcggcgcga cccatatctg cacgcccttg
     3481 ccgtacagct cgtagcgcgc gaggggcatc cggttctccc aacagatcag acctccgacg
     3541 cgtccgagct cggtctcgac cacgtcgaga tcggtaccgt cccccgtccc gtggaagagg
     3601 cgctcgtgca tcgtggggac gagcttgcgg tgcttggcga gcaacccgcc ggggcccatc
     3661 agcaagagcg tgttgtagag cgagccgggg cgctcgagct ctcgctcgtt cactccgatc
     3721 gcgcagtgga tcccgcgttc cgcgcaggcg gcgagcagct tgtccacggc gggaccgggg
     3781 acctcgacgg acgaagccca gaggcgctcc cacatctcgt cgaagccgcc gaaccgggac
     3841 gcgcgatgcc cccacgcgcc gctgggataa accgagacga aggtctcggg gaagacggcc
     3901 agctggacgc cctcgtccgc cgcgcggtgc agaaggccga gcgctttgtc gatcgtcgcg
     3961 tcggcgtcga ggatcacggg gcaggcctgg atcgcgccga ccttgacggt ctcgaatacg
     4021 ggcatgcccg cgacgttagg cgacgttctc tcgacaggtc aagctcagcc ggcgcaggcg
     4081 tcccttatcg cggcgacgtg gcggtggtcg gtgccgcagc accctccgag cacgttcagc
     4141 ccgggaagcg acgtacgcag ctcggcgtag cggcgagcga ggtcggccgg gtccccttcg
     4201 tcgaggtcct cggcctcgtc gagctcggcg tggctccgcg tcgacg
//
LOCUS       DASQDO010000285         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_764942, whole genome shotgun sequence.
ACCESSION   DASQDO010000285 DASQDO010000000
VERSION     DASQDO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764942"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VEV43_08005"
     CDS             complement(<1..846)
                     /locus_tag="VEV43_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYP23501.1"
                     /translation="MPLVDAPRTRRSFARLNRVQDVPNLIDIQRRSFAWLTDPEKGGL
                     RETISDISPIEDYTGNLAVDFGEFHFDDPVASIEECREKDLTYARPLTVTVAFVNRET
                     GEIREQSVFMGDFPWMTERGTFIINGTERVVVTQLVRSPGAYLMEAKDREKQVFIANL
                     MPARGSWLELEIDKKGKVFVRIDRKRKLPVTVLLRAMGYATDEEIINLFDNSLYIRNT
                     IDADTEPTKTEEGALVELFTTQRPGEPPSVANARALLHPLFFDPKRSDLTRVGRYKLN
                     ARLGLD"
     gene            1081..1419
                     /locus_tag="VEV43_08010"
     CDS             1081..1419
                     /locus_tag="VEV43_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP23502.1"
                     /translation="MKFDHVAQPVPDIAAALDWWQRTVPDARVLYADETWGLLEAGGA
                     KLAFVLAEQHPQHLAFAVSNATLERLAGEHGAEIALHRDGSRSFYLEAPGDGRLVIVA
                     YPDALEEDFE"
BASE COUNT          257 a          538 c          590 g          257 t
ORIGIN      
        1 gtcgaggccc aggcgcgcgt tgagcttgta gcgcccgacg cgcgtgaggt cggagcgctt
       61 gggatcgaag aagagcgggt gcagcagcgc acgcgcgttg gcgaccgacg gcggctcgcc
      121 ggggcgctgc gtcgtgaaca gctcgacgag cgcgccctcc tcggtcttcg tcggctcggt
      181 gtcggcgtcg atcgtgttgc ggatgtagag gctgttgtcg aagaggttga tgatctcctc
      241 atcggtggcg tagcccatcg cgcgcagcag cacggtgacc ggcagcttgc gcttgcggtc
      301 gatgcggacg aagaccttgc ccttcttgtc gatctcgagc tcgagccacg agccgcgagc
      361 cggcatgagg ttcgcgatga agacctgctt ctcgcggtcc ttggcctcca tgaggtacgc
      421 gcccggcgaa cggacgagct gcgtgacgac gacgcgctcc gtgccgttga tgatgaacgt
      481 gccccgctcg gtcatccacg gaaagtcgcc catgaacacc gactgctcgc ggatctcacc
      541 ggtctcgcgg ttgacgaacg cgacggtgac ggtcagcggg cgggcgtagg tgaggtcctt
      601 ctcgcggcac tcctcgatgg aggcgacggg atcgtcgaag tgaaactcgc cgaagtcgac
      661 ggcgaggttg ccggtgtagt cctcgatcgg cgagatgtcc gagatggtct cgcgcagacc
      721 acccttctca gggtcggtca gccacgcaaa cgagcggcgc tggatgtcga tgaggttcgg
      781 gacatcttga actcggttca gacgcgcgaa gctccggcgc gtccgggggg cgtcaacaag
      841 gggcaaggcg gcgactcctc aaggggtcgg agagggcgat cattcggggg gcagcgccgc
      901 ggccgtcggc ctgcaaggcg cggactcgcc aggatagcac agcaaagtgc ggcccccgca
      961 ggggggctgt cgacgctcac ggaccccgcc tcaagtctgc gctatgtggg cgcaaaaccg
     1021 tgctgaaggc ggctacggct atcggtcgac ccgcgcaatg tagcgcgtat cgtgcggatc
     1081 gtgaaattcg accacgtcgc ccagcccgtg cccgacatcg ccgccgcgct ggactggtgg
     1141 cagcgcacgg tgccggacgc gcgcgtgctg tacgccgacg agacgtgggg cctgctcgag
     1201 gccggcggcg cgaagctcgc gttcgtgctc gccgagcagc acccccagca cctggcgttc
     1261 gccgtgtcga acgcgaccct cgagcgcctg gccggcgagc acggcgccga gatcgcgctg
     1321 caccgcgacg gcagccgctc gttctacctc gaggcaccgg gcgacggtcg gttggtgatc
     1381 gtcgcctacc ccgacgcgct ggaggaggac ttcgagtagg gcgtccccga gaagcgtccc
     1441 ggctatggat gcctctcgcg ccctcagaga tccgcccgcc ggaacatccg gtcggccagc
     1501 catcccgcgg ccgccaggta cgcgagcgcg aacgggtaca gcagcggccc ggcctcctgg
     1561 gaccccccga acgggccgag ctgcacgatg acgcccgtgt cgccctcgat gtcgaccgtc
     1621 agggcgtgca ggccgtcttg gt
//
LOCUS       DASQDO010000286         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_765010, whole genome shotgun sequence.
ACCESSION   DASQDO010000286 DASQDO010000000
VERSION     DASQDO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765010"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="proC"
                     /locus_tag="VEV43_08015"
     CDS             <1..720
                     /gene="proC"
                     /locus_tag="VEV43_08015"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HYP23503.1"
                     /translation="TARTQERLDALAGSYGVQTTLSNPEAVAGAQVVVLAVKPQDAEA
                     LLADVSPHVGEDHTLLSVVAAISTAFIESHLAGPVPVVRAMPNTPSIVHEGMAGIAGG
                     KHATDEHVALAREVLTHVGRVAVVPESYLDAVTAISGSGPAYFALLAEAMIEAGILLG
                     LAREMSTELVVQTMLGSAKLLRDEKMHPVELREMVTSPGGTTIRAIRVLEQSGVRAAF
                     LNAIQAAMERSQELAAGQPEA"
     gene            733..1215
                     /locus_tag="VEV43_08020"
     CDS             733..1215
                     /locus_tag="VEV43_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP23504.1"
                     /translation="MRVRRAVPDDLVELLDMYETVAAERIHIGGEAPVDRERRRTAWL
                     DTMDPETPGTSLVATVDGEIAGQLGLTGAGRMQLGMWVAQEWRGKGVGSALVRAALEH
                     ARANGAYKVSLEVWPHNAAAIALYEKFGFEREGYLRKHWRRRNGELWDSVVMGLVLDD
                     "
     gene            1208..2329
                     /locus_tag="VEV43_08025"
     CDS             1208..2329
                     /locus_tag="VEV43_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HYP23505.1"
                     /translation="MTEGSVALVTVEDIARAYDFGSSHPLRPERVLLTYDAVRELGLT
                     ALPNVSEVPSRSATDDEILAVHDEDFVAMVKAVDAGAADRREALEFGLGTPDNPIFDG
                     MHRASAAVCGASITAAEAVATGRVQHAFNPAGGLHHARRREASGFCIYDDPAAAIAKV
                     LELHPDWRVMYVDVDVHHGDGVQWIFYDDPRVLTVSFHQSGRYLYPGTGFEDEIGEGD
                     ARGTSANVPLMPLTGEDEYLWALEQVVPALAEAFSPHLVLTQLGADTHYGDPLANLGL
                     TMPAYPRMARILHETADRFAGGRWVATGGGGYQAETVVPKVWTIHFAEMCGRPDLVPP
                     EWLEDTDPAEVSRSYRGTVERSVAQVLEACVPRLSALAG"
     gene            2368..>3745
                     /locus_tag="VEV43_08030"
     CDS             2368..>3745
                     /locus_tag="VEV43_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYP23506.1"
                     /translation="MGRTKTWGVVGALVVAAASAGGAPGRAAQSDPPARPNVLIVITD
                     DQRASGTMGVMPLTQRWFSDGGTHYVNAIATTPTCCPSRASVMTGLYAHNHGVHTSQA
                     GEAENMDQLLTLQRYLRRDGYLTAIYGKYLNAWPREIDPPWFDDWAILSDSTARAYFN
                     GRWNVNGKIGKLKPYSTRIIERKALRFLAASEAADDQPWLMYLAPMSPHLPAKPHPLH
                     RGIDVPAFRKTPAMKEEDRSDKPQEVQQQEVDWKTVREWRRKQLRSLMSVDEMVGAVF
                     RRLRRTGEARDTLAIYLSDNGFLWGEHGLGQKIRPYEASAEVPFFVRWPGHVPAGEVD
                     ERLVANIDIAPTVMEAARSDAEHAYDGRSFLGPPHDRVLLEQWKRPDRSVPNWAATRA
                     DDYLYVEYYAEDRLIPAFREYYDLVEDPWELHNLLGDLDPTNDPPDAVDLSLQLHRDV
                     ICAGTSGPS"
BASE COUNT          572 a         1314 c         1350 g          509 t
ORIGIN      
        1 accgcacgca cgcaggagcg cctcgacgcc ctggccggct cgtacggcgt gcagacgacc
       61 ctcagcaacc ccgaggcggt ggcgggggcc caagtggtcg tgctggcggt caagccccag
      121 gacgccgaag ccctgctcgc cgacgtctcg ccccacgtgg gcgaggacca cacgctgctg
      181 tcggtggtcg ccgcgatctc gacggcgttc atcgagtcgc acctggccgg cccggtgccg
      241 gtcgtccgcg cgatgccgaa caccccctcg atcgtccacg agggcatggc cgggatcgcc
      301 gggggcaagc acgcgacgga cgagcacgtg gcgctcgcgc gggaggtgct cacgcatgtc
      361 gggcgagtag ccgtcgtccc cgagagctac ctcgacgccg tgaccgcgat ctccgggtcc
      421 ggccccgcgt acttcgcgct ccttgccgag gcgatgatcg aggcgggcat cctgctcggc
      481 cttgcccgcg agatgtcgac cgagctcgtc gtccagacga tgctcggctc ggcgaagctc
      541 ttgcgcgacg agaagatgca ccccgtcgag ctgcgcgaga tggtgacgtc gcccggcggg
      601 accacgatcc gggcgatccg tgtgctcgag cagtcgggtg tgcgggccgc gttcctcaac
      661 gcgatccagg cggcgatgga gcggtcgcag gagctcgcgg cgggccagcc cgaggcctag
      721 accgtggcgg atttgagggt caggcgggcg gtacccgacg acctggtgga gctcctcgac
      781 atgtacgaga ccgtcgcggc cgaacggatc cacatcgggg gcgaggcacc ggtcgaccgc
      841 gagcgccgca ggacggcctg gctcgacacg atggacccgg agacgccggg gacctcgctg
      901 gtggccacgg tggacgggga gatcgcgggc cagctcggcc tcacgggggc cgggcggatg
      961 cagctcggga tgtgggtcgc gcaggaatgg cggggcaagg gcgtcggctc ggcgctcgtg
     1021 agggcggcgc tcgaacacgc ccgggcgaac ggcgcctaca aggtctcgct cgaggtctgg
     1081 ccgcacaacg ccgcggcgat cgcgctctac gagaagttcg ggttcgagcg cgaggggtac
     1141 ctccgcaagc actggcggcg ccgcaacgga gagctgtggg actcggtagt gatggggctg
     1201 gtgctggatg actgaagggt ccgtcgccct cgtcaccgtc gaggacatcg cccgggccta
     1261 cgacttcggc tcgtcacacc cgctccggcc cgagcgggtc ctcctcacct acgacgccgt
     1321 ccgcgagctc ggcctgaccg cgctgccgaa cgtctcggag gtcccgtcgc gctccgcgac
     1381 cgacgacgag atcctcgcgg tgcacgacga ggacttcgtc gcgatggtca aggccgtcga
     1441 cgcgggggcc gcggaccggc gggaagcact cgagttcggc ctcggcaccc cggacaaccc
     1501 gatcttcgac ggcatgcacc gggcctcggc cgcggtctgc ggcgcttcga tcacggcggc
     1561 cgaggccgtg gcgaccggac gggtgcagca cgcgttcaac cccgccggcg ggctccacca
     1621 cgcgcggcgc cgcgaggcgt cggggttctg catctacgac gatcccgccg ctgcgatcgc
     1681 gaaggtcctc gagctccacc cggactggcg cgtcatgtac gtcgacgtcg acgtccatca
     1741 cggcgacggc gtgcagtgga tcttctacga cgacccgcgg gtgctgacgg tctcgttcca
     1801 ccagtcgggc cgctacctgt atccggggac cgggttcgag gacgagatcg gcgagggcga
     1861 cgcgcgcggc acgtcggcga acgtcccgct gatgccgctg accggcgagg acgaatacct
     1921 ctgggccctc gagcaggtcg tgccggcgct ggccgaggcc ttctcgcccc acctcgtcct
     1981 cacccagcta ggcgccgaca cgcactacgg agacccgctg gcgaacctcg gcctgaccat
     2041 gccggcctac ccccgcatgg ctcggatcct ccacgagacc gcggaccgct tcgccggggg
     2101 gcgctgggtg gcgaccggcg ggggcggcta ccaggccgag accgtcgtgc cgaaggtctg
     2161 gacgatccac ttcgccgaga tgtgcggccg tccggacctg gtgccgcccg agtggctcga
     2221 ggacaccgat ccggcggagg tctcccgcag ctaccgcggg acggtcgagc gctcggtggc
     2281 ccaggtgctc gaggcgtgcg tgccccggct gtcggcgctc gccgggtagc gcgttacgcc
     2341 tcctagtgcg ggagagtacc cacggtcatg gggcgaacga agacgtgggg tgtggtcggt
     2401 gcgctcgtgg ttgccgccgc gagcgccggg ggcgcgcccg gccgcgcggc gcagagcgac
     2461 ccgccggccc ggccgaacgt gctgatcgtg atcaccgacg accagcgtgc ctccgggacg
     2521 atgggggtga tgccgctcac gcagcggtgg ttctcggacg gcgggaccca ctacgtgaac
     2581 gcgatcgcga cgacgccgac gtgctgcccc tcgcgcgcct cggtcatgac cgggctctac
     2641 gcgcacaacc acggcgtcca cacgagccag gccggagagg ccgagaacat ggaccagctg
     2701 ctgacgctgc agcggtatct ccgcagggac ggctacctca cggcgatata cggcaagtac
     2761 ctcaacgcgt ggccccgcga gatcgacccg ccgtggttcg acgactgggc gatcctgtcc
     2821 gacagcaccg cccgcgccta cttcaacggg cgctggaacg tgaacggcaa aatcggcaag
     2881 ctgaagccct actcgacccg gatcatcgag cggaaggcct tgcgtttcct cgccgcgtcg
     2941 gaggcggcgg acgaccagcc gtggctcatg tacctcgcgc cgatgagccc gcacctcccc
     3001 gcgaagccgc acccgctcca ccgcgggatc gacgtccccg cgttccgcaa gacgccggcg
     3061 atgaaggagg aggaccgcag cgacaagccg caggaggtcc agcagcagga ggtggactgg
     3121 aagaccgtgc gtgagtggcg gcgcaagcag ctgcgcagcc tgatgtcggt cgacgagatg
     3181 gtcggggccg tgttccgccg gctgcgccga accggcgagg cccgcgacac gctcgcgatc
     3241 tacctgtcgg acaacggctt tctgtggggt gagcacggcc tcggtcagaa gatcaggccg
     3301 tacgaagcgt ccgccgaggt gccgttcttc gtgcggtggc cgggacacgt cccggcgggc
     3361 gaggtcgacg agcggctcgt cgccaacatc gacatcgcgc cgaccgtgat ggaagccgca
     3421 aggtccgacg ccgagcacgc ctacgacgga cggtcgttcc tcggcccccc acacgatcgg
     3481 gtcctcctcg aacagtggaa gaggccggac cggtcggtgc cgaactgggc cgcgacgcgt
     3541 gccgacgact acctgtacgt cgagtactac gccgaggacc ggctcatccc ggccttccgc
     3601 gagtactacg acctcgtcga ggacccgtgg gagctgcaca acctcctcgg cgacctcgac
     3661 ccgacgaacg acccgccgga cgcggtcgac ctgtcgctgc aattgcaccg cgacgtgatc
     3721 tgcgcgggca cgtcgggccc gtcgg
//
LOCUS       DASQDO010000287         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_947930, whole genome shotgun sequence.
ACCESSION   DASQDO010000287 DASQDO010000000
VERSION     DASQDO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947930"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1349
                     /gene="hisS"
                     /locus_tag="VEV43_08035"
     CDS             93..1349
                     /gene="hisS"
                     /locus_tag="VEV43_08035"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYP23507.1"
                     /translation="MPEDRSFTAPKGTYDVLPPESWRWVEGARRALDTFARSGYAPVE
                     TPAFEQTEVFERGVGATSEVVGKQMYTFLDKGGRSLTLRPEGTAPVMRAILEHRLERG
                     PLPVKLAYASFMFRQERPQKGRFRQFFQVGIENVGSDDPAVDAEVVEVGARYLRAMGV
                     RSTLLLNSIGHPSESCRGGYVKLLADWLRDRVDDLAPADRERVDTNPLRTFDSKEAAT
                     NAVMTDAPLITDHLCESCASHFGDVRALLDEVDVGYELEPRLVRGLDYYTRTAFEYVS
                     GDLGSQSAVGGGGRYDGLAESLGGDPLPGIGFALGLDRILLASEAAADAPEAVDAYVV
                     AATPEARRPAFVLATRLRGAGIGADFDATGRGMKGQMKDAARSGARWAAILGPAELEA
                     GAVTLRDLSSGDQEQVPLEDLERRLV"
     gene            1350..3125
                     /gene="aspS"
                     /locus_tag="VEV43_08040"
     CDS             1350..3125
                     /gene="aspS"
                     /locus_tag="VEV43_08040"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYP23508.1"
                     /translation="MLRSHTCGQLRATDEGATVTLCGWVHSRRDHGGVAFVDLRDTTG
                     LTQVVLGTPELVGSIRNEYCVKVTGTVVRRKEGTVNPKLATGEVEVTADSLEVLSAAE
                     TPPFQVDDHADVDETLRLKYRFLDLRRDRMQENLKLRHKIVSAIRRFYDSEGFVEVET
                     PMLTRATPEGARDYLVPSRVHPGKFYALPQSPQLFKQLLMVSGLDRYYQIVRCFRDED
                     LRADRQPDFTQLDVEMSFVDVEDVLDSTERMFRYVWREALGVDLPPFARLSYHESMER
                     FGVDKPDLRFGMELVDLADVFAATEVKVFRSVLDGGGAVKAIAVGGKGDLARKELDGI
                     VEEAKALGAGGLVWIAVQADGFKSPIDKFFTDDERAGLLKATSAGEGDLILIVADKRA
                     SIVYKVLGALRNRLAARFGFVPERDRTDPDAWKFAWITDFPWFERDEEEDRWTPIHHP
                     FTGVVEDTMQYLDSDPGKVISKSYDITLNGWELASGSIRIHDGELQQKMFDALGITPE
                     IAEEKFGFLLRAFRYGPPPHGGIAPGIDRIVALAAGETNIREVIAFPKTQTAYDPLTD
                     APSGVDDAQLKVLHLKSTAPPPQTG"
     gene            3132..>3641
                     /locus_tag="VEV43_08045"
     CDS             3132..>3641
                     /locus_tag="VEV43_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP23509.1"
                     /translation="MDLFDSALEQRFGEYAPLAARMRPRTIDEVVGQKHLLGPGKALR
                     ALVESGSSSSIVLWGPAGTGKTTIAHVIAGTADAHFEPMSAVTSGVADVRKAIEAARD
                     RLGQQGRRTVLFLDEIHRFNKAQQDALLPAVENGWIVLVGATTENPSFEVNSPLMSRS
                     LLFRLEALTR"
BASE COUNT          557 a         1220 c         1335 g          529 t
ORIGIN      
        1 gatcgtccgc acccttcgtg tactgggaac gccggccgca cgccggtatt ccgggaatcg
       61 ggccggggag gagcggcccg taggatggtt cgatgccgga agaccgttcc ttcaccgcgc
      121 ccaaggggac gtacgacgtc ctgccgcccg agtcgtggcg gtgggtcgag ggggcccggc
      181 gggcgctcga cacgttcgcc cgctccggct acgcgcccgt cgagactccc gccttcgaac
      241 agaccgaggt cttcgagcgc ggcgtcgggg cgaccagcga ggtcgtcggc aagcagatgt
      301 acacgttcct ggacaagggg gggcggtcgc tgacgctgcg gcccgagggg acggcgccgg
      361 tgatgcgggc gatcctcgag caccggctcg agcgcggccc cctgccggtc aagctcgcct
      421 acgccagctt catgttccgc caggagcgtc cccagaaggg gcggttccgg cagttcttcc
      481 aggtcgggat cgagaacgtc ggctcggacg acccggcggt cgacgccgag gtcgtcgagg
      541 tcggggcccg ctatctgcgc gcgatggggg tgcgctcgac gctgctgctg aactcgatcg
      601 ggcacccgtc ggagtcgtgc cggggcgggt acgtgaagct gctcgcggac tggctgcgcg
      661 accgggtcga cgacctggcg ccggccgacc gcgagagggt cgacacgaac ccgctgcgga
      721 cgttcgactc gaaggaggcc gcgacgaacg cggtcatgac cgatgccccc ttgatcaccg
      781 accacctctg cgagtcctgc gcctcgcact tcggcgacgt ccgggccctg ctcgacgagg
      841 tggacgtcgg ctacgagctc gagccccggc tcgtgcgggg gctcgactac tacacgagga
      901 cggcgttcga gtacgtgtcg ggtgacctgg gctcgcagag cgcggtcggg ggcgggggcc
      961 gctacgacgg tctcgccgag tcgctgggtg gcgacccgct gcccggcatc ggcttcgcgc
     1021 tggggctcga ccggatcctg ctcgcgagcg aggccgcagc ggacgcgccc gaagcggtgg
     1081 acgcatacgt cgtcgccgcg acgcccgagg cacgccgccc ggccttcgtc ctggcgacgc
     1141 ggctgcgcgg agccgggatc ggcgccgact tcgacgcgac cgggcggggc atgaaggggc
     1201 agatgaagga cgcggcgcga tcgggcgcac ggtgggcggc catcctcggc cccgccgagc
     1261 tcgaagccgg cgccgtgacg ctgagggacc tgagctccgg cgaccaggag caggtgcccc
     1321 tcgaggacct cgagaggaga ctcgtctaga tgttgcggtc acacacgtgc ggtcagctgc
     1381 gcgcgacgga cgaaggggcg actgtcacgt tgtgcggctg ggtccacagc cgacgcgacc
     1441 acggcggcgt tgcgttcgtc gacctgcgcg acacgaccgg gctgacgcag gtcgtgctcg
     1501 ggacccccga gctcgtcggc tcgatccgca acgagtactg cgtgaaggtg accgggaccg
     1561 tcgtccggcg caaggagggg acggtcaacc cgaagctcgc gaccggcgag gtcgaggtca
     1621 ccgccgactc cctcgaggtg ctgtcggccg cggagactcc gccgttccag gtcgacgacc
     1681 acgccgacgt cgacgagacc ctgcggctca agtaccggtt cctcgacctc cggcgagacc
     1741 ggatgcagga gaacctcaag ctgcgacaca agatcgtctc ggcgatccgg cggttctacg
     1801 actccgaggg cttcgtcgag gtcgagacgc cgatgctcac gcgggcgacg cccgagggcg
     1861 cacgcgacta tctcgtgccg tcgcgggtgc accccggcaa gttctacgcg ctcccgcagt
     1921 cgccgcagct cttcaagcag ctgctgatgg tgtcggggct cgaccgttac taccagatcg
     1981 ttcgctgctt ccgcgacgag gacctgcgcg cggaccggca gccggacttc acgcagctcg
     2041 acgtcgagat gtcgttcgtc gacgtcgagg acgtgctgga ctcgaccgag cggatgttcc
     2101 ggtacgtgtg gcgcgaggcg ctgggcgtcg acctgccccc gttcgcgcgg ctgagctacc
     2161 acgagtcgat ggagcgtttc ggggtcgaca agccagacct ccgcttcggc atggagctcg
     2221 tcgacctcgc ggacgtgttc gccgcgaccg aggtgaaggt gttccgctcg gtgctcgacg
     2281 gcggcggtgc ggtgaaggcg atcgccgtcg gcggcaaggg cgacctcgcg cgcaaggagc
     2341 tcgacgggat cgtcgaggag gcgaaggcgc tgggggccgg gggcctcgtg tggatcgccg
     2401 tccaggcgga cggcttcaag tcgccgatcg acaagttctt caccgacgac gagcgggccg
     2461 ggctgctgaa ggcgacgagc gcgggggagg gcgacctcat cctgatcgtc gccgacaagc
     2521 gcgcgtcgat cgtctacaag gtgctcggcg cgctgcgcaa ccggctggca gcgcgcttcg
     2581 ggttcgtccc cgagcgcgac cgcaccgatc ccgacgcttg gaagttcgcg tggatcaccg
     2641 acttcccgtg gttcgagcgt gacgaggaag aggaccggtg gacgccgatc caccacccgt
     2701 tcacaggcgt ggtcgaggac accatgcagt acctggactc ggaccccggc aaggtgatca
     2761 gcaagtcgta cgacatcacc ttgaacgggt gggagctcgc cagcgggagc atccgtatcc
     2821 acgacgggga gctccagcag aagatgttcg acgcgctcgg gatcactccg gagatcgccg
     2881 aggagaagtt cggcttcctg ctgcgagcgt tccggtacgg gcccccgccg cacgggggca
     2941 tcgcgccggg gatcgaccgg atcgtcgcgc tcgctgcggg cgagacgaac atccgggagg
     3001 tcatcgcgtt cccgaagacc cagaccgcct acgatccgct gacggacgcg ccctcgggtg
     3061 tcgacgacgc gcagctgaag gtcctgcatc tgaaatccac cgctccgcct ccgcagacgg
     3121 gatagagact catggacctc ttcgacagcg cgctggagca gaggttcggc gagtacgcgc
     3181 ccctggccgc ccggatgcgg ccccgcacga tcgacgaggt cgtgggacag aagcacctgc
     3241 tcgggccggg caaggcgctg cgtgccctgg tcgagtcggg ctcgtcgtcg tcgatcgtcc
     3301 tgtgggggcc ggcgggcacc ggcaagacga cgatcgccca cgtgatcgcg gggaccgccg
     3361 acgcgcactt cgagccgatg tccgcggtga cgtccggcgt cgccgacgtg cgcaaggcga
     3421 tcgaggcggc gcgcgacagg ctcggtcagc agggccggcg cacggtcctg ttcctcgacg
     3481 agatccaccg cttcaacaag gcgcagcagg acgcgctgct ccccgccgtg gagaacggct
     3541 ggatcgtgct cgtgggtgcc acgaccgaga acccctcgtt cgaggtgaac tcgccgctga
     3601 tgtcgcgcag cctgctgttc cggctcgagg ccctgacccg g
//
LOCUS       DASQDO010000288        15405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_213897, whole genome shotgun sequence.
ACCESSION   DASQDO010000288 DASQDO010000000
VERSION     DASQDO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213897"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..650)
                     /locus_tag="VEV43_08050"
     CDS             complement(129..650)
                     /locus_tag="VEV43_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HYP23510.1"
                     /translation="MKIVFVCTGNICRSPMGEALLRHELAQRGCEDVEVSSCGTWASA
                     PQPATAHAVTVLHGRGIDLTAHRSRALEAAELEEADLVLAMTSVHVREMEEMAPAALP
                     KVRLMKELVELDLPPGAGLAELLAATRPAPRRDLDVDDPMGLLPRSYERAAGEIEAGV
                     KVVASVLCGERDQ"
     gene            674..2164
                     /locus_tag="VEV43_08055"
     CDS             674..2164
                     /locus_tag="VEV43_08055"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HYP23511.1"
                     /translation="MSRAWDELLPRLHQIDHLASTVRLLHWDQAAMMPPAGAPARAGA
                     IATLQGLIHERLTEPEVGGLLDELAADDSLDDDQKAHVRVFRREYEKATKVPPELVKE
                     LAEVEGLAYQAWTQARPASDFSMLQPHLERMVQLKKDQADALGWEGERYDALLDTYEP
                     DMTTAEVEAMFAELGEGLRPLVEEVLEGAPHPPEFLTRTYDAAKQEAFSQWLVVQLGF
                     DTNAGRLDTSPHPFTASVAAGDVRQTTKADPRDLMMSIFATIHETGHALYEQGIPDRL
                     AGLPASRPASLGLHESQSRMWENQVGRGRAFTEFMLPNLKEHFPEELGMLAPEDFYRG
                     ANHPRRTMIRVTADELTYNLHVALRFEIELALFRDELAVADLPDAWDAGMEKHLGIRP
                     ESHSDGVLQDMHWSIGALGYFPTYTLGNLYAAAFYARAREDLPDLDEQLRAGDGSSLL
                     SWLREKVHVHAYLRPAKEIGEEIIGGPLTAQPLLDYLANKFRSLYA"
     gene            complement(2145..2717)
                     /locus_tag="VEV43_08060"
     CDS             complement(2145..2717)
                     /locus_tag="VEV43_08060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYP23512.1"
                     /translation="MAGLEEAVDAIRDGALVIVPTDTVYGIGARPEAAAAIFDVKQRP
                     RDKALPVLGSDVVQLGGIAELDDSARALADEHWPGPLTLVVRRKESFDADLGGNDPAT
                     VAVRVPAHPIARALLEQTGPLAVTSANVSGEPPATTYEEACALAPDLVCLDGGTCDGV
                     PSTVLTLVGEPRVLRQGGLDLTDWLRRTAT"
     gene            complement(2705..3454)
                     /gene="prmC"
                     /locus_tag="VEV43_08065"
     CDS             complement(2705..3454)
                     /gene="prmC"
                     /locus_tag="VEV43_08065"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYP23513.1"
                     /translation="MRAGGVESAEAEARWIEQGAGGRDVDELVRRRLAGEPLQYVLGT
                     AAFRRLELKVGPGVFIPRPETELVAERAMARLPREGILVDLCTGSGAIALSVADERPD
                     ARVFATELSPTASQWATSNRDSLGLRVEVVQGDLFAALPADLAGRVDVVVSNPPYVDH
                     GERSLLPRDVVDHEPPEALFAPEEGTSVIARVAGEAPAWLRPDGWLLLEIGETQGDQV
                     RALLAARGYEDVAIEPDLNGRDRIAEARWPG"
     gene            complement(3502..4578)
                     /gene="prfA"
                     /locus_tag="VEV43_08070"
     CDS             complement(3502..4578)
                     /gene="prfA"
                     /locus_tag="VEV43_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HYP23514.1"
                     /translation="MKLPEDRLNEMEKRFGEIERLLSDPGLADRPDELRALGKEHSDL
                     RPTVDAWHRYKAAADDLEQAKEMLSGASPDERAYLEEEISTQSDERDRLAEEIAAALI
                     PKDPNDDKDVIVEIRAGAGGDEAGLFAADLYRMYSRYAETRRWKTEVLTSNETGVNGF
                     KEVTFAVKGKGAYSRLKHEAGVHRVQRVPVTESSGRIHTSAAGVMVLPEAEEVDVEID
                     PKDLKIDVYRSSGPGGQSVNTTDSAVRITHLPTGVEVACQDEKSQLQNKAKAMRILRA
                     RLLQAKQEEQQKAAAEERKSQVRTGDRSERIRTYNFPDNRVTDHRIGYTTHRLGEILE
                     GDIDDLVDALLRSDRAESLGDDGS"
     gene            4657..6642
                     /locus_tag="VEV43_08075"
     CDS             4657..6642
                     /locus_tag="VEV43_08075"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYP23515.1"
                     /translation="MSTTRRTFIRGIAAATAVVAGRAAPVLARRRKAKRKKPEPQWLF
                     RDQFQRRDSAGWGWPWFNQRYGRRWAISDRRGVYRLPATENNVYYRPNPIFVLDHDVA
                     NADLRATISMSNEQARFGIVARGTGYAEYYAAHIGPGNHIRLVRCGHHDEKVLARKPF
                     YVEANRRYRIRFQIRGAAPVRLKVKAWPVTEPEPRTWNIDTADPSPGALTTPGPFGLF
                     AEHGVDGRGAALRVSDFVAWSQEKPSYTQASITYAMAGPIQGSRVKVVAKCAFPARVG
                     FDYGADATFSNVRRVWAGNSSDRAQTASAWLDLEGFDAGSVVHWRAWALRRGKRVFGP
                     PSSLRMPPPPGLPVKFAFGSCTRWKPSPRPSVDHARLQLPDFYLHQGDLGYVPHRVIT
                     HAPDTYQDHWTRMFMDPSFQAMTRSVPVGVSQDDADYGTNLADRGSLRRFTIRAHDEL
                     TANPRSAYYTFRCGDMAVFVIDSRRYSTGKNVSPARRTKLGAEQKQWLFDEMTKASED
                     DVGLCVVASPQAFGSDRSPASWRKGFENEWRELMDFFEELVTPVLIVSGDAHGHRLHE
                     YPQKNLDPNVPRIVELVSSGTEQNKFSDDVDPRIVLRQAKGSGFGFVELGAEETTGGQ
                     RVRTLTLSALRSTDGAPLWAPQRYLIVRGVGILPLIL"
     gene            6805..7746
                     /locus_tag="VEV43_08080"
     CDS             6805..7746
                     /locus_tag="VEV43_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23516.1"
                     /translation="MILGKNKTIAALAATAIVASGALLAPAAQAATNCLPVPGSTLGS
                     DVKVGPVEERVPAISDIVVCAGGGTVPVARVATSGGTCTTSCITVYVGGSAVDLDGAS
                     ISWSQDDVPQPEVGLNPGPVGTGGEVCALSVGFPDAPDTDCTIAIGPDDPTGHLEEAG
                     ELVDTVVGLVTTAGEITCNQIPDWYDPETGSYADFCSQPDLWATAVANQGGELACGAI
                     PVMYDDWGNSYEFCDDATGWTTAFIDDTYETCNYTLGPQYDEYWWDYVYFCDDPIRWT
                     QLALENLCRNLCDVQTMQRLITTIRELANENVQIRIG"
     gene            7956..8741
                     /locus_tag="VEV43_08085"
     CDS             7956..8741
                     /locus_tag="VEV43_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HYP23517.1"
                     /translation="MSIVALGALWAPPAQAATCQSVPGSTTGLDLSVAGQQRRVPAIS
                     GITVCVDSPVIPLVTVSTSGNGNCTVGCLSVLSSGGDFDTGAVTLSYREDGSTRSQTV
                     SGGSLGGPDGSCLLSVGAPDAPYPTCFVALGIDDLPVDPDEIQEDLDQLQEDLGQDVD
                     ELQEDLEDLQGDLGDQVDQLEEDLGNLEQTACDTIPPLQDPNSWEEYRFCDDPAAWAG
                     VITEGVLLFIRDTVRPIQDLIRDRPTPQEIAEIVCDALLEPSC"
     gene            complement(8807..9760)
                     /locus_tag="VEV43_08090"
     CDS             complement(8807..9760)
                     /locus_tag="VEV43_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HYP23518.1"
                     /translation="MSEENPTPRPSIGGQAVLEGVMMRGPASWSVACRRPDETISVEK
                     HPLPSVAERHPWLKWPLARGVLVLGESLAIGIKALMISANQALEEEEQLSDKQLGWTL
                     GTAMLLFSAIFIALPVFGTRLIENLTGNLSEDNPLVANLIEGGIRLSMFVGYLLVIGT
                     FKDVRRVFQYHGAEHKTIYAYENGDPLDPKLIDDKYPTLHVRCGTNFLFIVLFLTIIA
                     HFVIDLLFAHLGLLPRVGIRIAAIPVLAGISYEAIKLASKNENSLFFRISMLPGLALQ
                     RITTKPPSLDQIEVAIAAMNAVAEDVEKPAGAGTIPDSPVA"
     gene            complement(9780..10079)
                     /gene="rpmE"
                     /locus_tag="VEV43_08095"
     CDS             complement(9780..10079)
                     /gene="rpmE"
                     /locus_tag="VEV43_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HYP23519.1"
                     /translation="MKQGIHPDYIETKVTCSCGNTFTTRSVKAELHVELCNQCHPFYT
                     GKQKLVDTGGRVERFQKKYANRAKPGGAASAGPQNEKGAEPAADAPATADASATE"
     gene            complement(10244..11968)
                     /gene="rho"
                     /locus_tag="VEV43_08100"
     CDS             complement(10244..11968)
                     /gene="rho"
                     /locus_tag="VEV43_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HYP23520.1"
                     /translation="MAETMDRAALESKAVAELKQIAKSMDLKVAGLKKAEIVDRIAGG
                     GSNGGSAAPRPARKPREKAPVAAPIGDAPAPAPAGAQAAAPAQPSTPSGDQGSSSDGG
                     TSPAAVRDEGAVDDRDRRNDDRQQRRRPQGRENRSRPREKAEPRDGRDNRGGGNDRGG
                     NDRGGSGRRRRRGRGGGAPGVPHYMRDERWDELEAEDDPNAEVRTGVLDLLPEGYGFL
                     RTSGYLPGDKDVYVSVSQVRRFHLRKGDEVEGSVRSPKESEKYQALLKIKTINGRPPE
                     EVQNRVRFADLTPLYPQDRLTLEQPDNPRSITPRIVDLIAPIGKGQRGMIVSPPKAGK
                     TTVMKEIANAVRANNPETHLIVLLVDERPEEVTDMQRSVDGEVVYSTFDRPSDDHIQV
                     TELTLERAKRLVEMGKDVMILLDGITRLSRAYNLATPASGRILSGGVDSTALYPPKKF
                     FGAARNIEFGGSLTILATALVETGSRMDEVIFEEFKGTGNWELKLDRRMSEKRIFPAI
                     DIQASGTRKEELLLTPEELQLVWRLRRVLHALADGGALELLIDKMKATMGNAQFLREI
                     AKAPVKGE"
     gene            complement(11978..12658)
                     /gene="fsa"
                     /locus_tag="VEV43_08105"
     CDS             complement(11978..12658)
                     /gene="fsa"
                     /locus_tag="VEV43_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HYP23521.1"
                     /translation="MKLFLDTANLDEIREGVRWGVLRGVTTNPSLIAKEKQGFLPLVR
                     DILSLVDGDVSIETASDDTDLLVRQGHEIAKMADNAVVKIALTPNGLAATRQLANDGI
                     KVNVTLAFTPAQAILAAEAGAYIVSPFVGRLDDVATDGLRLIADICEIFIVQGYDTQV
                     LAASLRHPIHVTEAARLGADIATMPFKVFEQLVRHPLTDSGFKRFTDDWEQAKPHMGE
                     LPPSMQEG"
     gene            complement(12642..13670)
                     /locus_tag="VEV43_08110"
     CDS             complement(12642..13670)
                     /locus_tag="VEV43_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP23522.1"
                     /translation="MNEKRRKHIFITESERGLPYSKGLTASSLMATGLGPGRAYAVAN
                     KVEEELRGAGSSYVTEEALRDLTVRVLRREAGDSYAQAFVKWQSVAELDVPLIILLGG
                     ATGVGKSTLATQLASRLGITRVVSTDAVREVLRSALTEAMFPTLYASSFEADSAVRQP
                     IPHSGDKLIIGFREQAAAVAVGAQALIDRAIQEGTDMILEGAHLVPGFLQSVESDKAI
                     VVPLLITIDDEDLHRSHFFRRGRDTSSRGGERYLNAFRKIRKLQKYMVSSALMRGVPA
                     IAHYDLDATLSEVIDHIVNKALESVEKGVDVGGTDEKLIEKAGEAVGRGRPPATEDAE
                     GGTREVVS"
     gene            complement(13651..15000)
                     /locus_tag="VEV43_08115"
     CDS             complement(13651..15000)
                     /locus_tag="VEV43_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HYP23523.1"
                     /translation="MVDGEHYPPVVESALDELAAGGDEVVAALLVGGREKLPAEGVEA
                     YGDVRVVLGEDPRVALDEFLRDNDVDAVVDLSDEPVLDYRRRHVLASIALWHDTPYQG
                     ADFELRPPRRPPLASKPSLAVIGTGKRTGKTAVAGFTARVLSSSGRHPVIVAMGRGGP
                     AEPEVLRGDEIDLEPKDLLEMADAGKHAASDYVEDALLGRVPTVGCRRCGGGLAGGVE
                     ISNVPAGIELANELPGDLLLLEGSGSSIPPCHADVSLLVVPASIPEEYLAGYLGPYRL
                     LLADFVVVTMCEEPFGSPSQVSRTTSLVRDSWSPGPKQEIELVRTVFRPAPTRSVQGA
                     EVVVATTAPEAAGDSIKRHLEGEHGCRVVGISHSLSDRGKLKQELKDAGQADVLLCEI
                     KAAGIDVATREALSSGMDVVYMDNVPVPIDDGDLEAMLVTAADLAAGRHREGEREAT"
     gene            15122..>15405
                     /locus_tag="VEV43_08120"
     CDS             15122..>15405
                     /locus_tag="VEV43_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23524.1"
                     /translation="MFGRTRDEARHEDRRVTGHEVVAARGGVSPGAILTGVVVAVGAM
                     FVLTAVSAGILVGLGVEDELTPGQATDVGIATGIGLVVAQFLSYMWGGYAA"
BASE COUNT         2320 a         5382 c         5240 g         2463 t
ORIGIN      
        1 cgaggacctc gtggccgagc tccttcaggc ggtggccgag ggcggacttc agcgcgtacc
       61 cggcgtggtc ggacccgagg gcgatcttca ccaccgcatc ctagaagaac gagcccgcgg
      121 cctcccggct attgatcgcg ctctccacac aggacggacg cgaccacctt cacccccgcc
      181 tcgatctcgc cggcggcccg ctcgtaggac cgcggcagga gccccatcgg gtcgtcgacg
      241 tcgaggtccc ggcggggggc gggccgggtc gccgccagca gctctgcgag ccccgcgccc
      301 ggagggaggt cgagctcgac gagctccttc atgaggcgga ctttcgggag cgcggccggt
      361 gccatctcct ccatctctct cacgtggacg ctcgtcatcg cgaggacgag gtccgcttcc
      421 tcgagctccg cggcctcgag ggcgcgggac ctatgggccg tcaagtcgat gccgcgcccg
      481 tgcaggacgg tgaccgcgtg cgccgtcgcg ggttgggggg ccgaggccca agtgccgcag
      541 ctcgagacct cgacgtcctc gcagccgcgc tgcgcgagct cgtggcggag cagcgcctcg
      601 cccatcggcg agcgacagat gttcccggtg cacacgaaga cgatcttcac gaccgaaagg
      661 gtacggtggc cgcatgtcta gggcgtggga cgagctgctg ccgcggctcc atcagatcga
      721 ccacctcgcg tcgacggtgc ggctgctcca ttgggaccag gccgcgatga tgccgcccgc
      781 gggagccccg gcccgcgccg gcgcgatcgc cactctgcag ggcctgatcc acgaacggct
      841 gaccgagccg gaggtcggcg ggctcctcga cgagctggcg gccgacgact cgctcgacga
      901 cgaccagaag gcgcacgtgc gggtcttccg ccgcgagtac gagaaggcga cgaaggtccc
      961 gcccgagctc gtgaaggagc ttgcggaggt cgagggcctc gcctaccagg cgtggaccca
     1021 ggcccgtccc gcgtcggact tctcgatgct ccagccacac ctcgaacgga tggtccagct
     1081 caagaaggac caggccgacg cgctggggtg ggagggcgag cgctacgacg ctctcctcga
     1141 cacgtacgag cccgacatga cgacggccga ggtcgaggcg atgttcgcgg agctcggcga
     1201 agggctgcgc ccgctggtcg aggaagtgct ggagggcgca ccgcacccgc cggagttcct
     1261 cacgcgcacc tacgacgcgg cgaagcagga ggcgttctcg cagtggctcg tcgtgcagct
     1321 cgggttcgac acgaacgccg ggcgtctcga cacctcgccg catccgttca cggcgagcgt
     1381 cgcggccggt gacgtccgcc agacgaccaa ggccgacccg cgcgacctga tgatgtcgat
     1441 ctttgcgacg atccacgaga ccggccacgc gctgtacgag cagggcatcc ccgatcgtct
     1501 cgccggcctc cccgcgagcc gtcccgcgtc gctcggcctc cacgagtcgc agtcgaggat
     1561 gtgggagaac caggtgggcc ggggccgtgc gttcaccgag ttcatgctcc cgaacctcaa
     1621 agagcacttc cccgaggagc tggggatgct cgcacccgag gacttctacc gcggtgccaa
     1681 ccatccgcgc cggaccatga tccgcgtcac cgccgacgag ctcacgtaca acctgcacgt
     1741 cgcgctgcgc ttcgagatcg agctcgcgct gttccgcgac gagctcgcgg tcgccgacct
     1801 gcccgacgcg tgggacgcgg ggatggagaa gcacctcggc atccggcccg agagccactc
     1861 cgacggcgtg ctgcaggaca tgcactggtc gatcggcgcg ctcggctact tcccgacgta
     1921 cacgctcggc aacctgtacg cggcggcgtt ctacgcgcgg gcgcgcgagg acctgccgga
     1981 cctggacgaa cagctgaggg ccggcgacgg ttcgagcttg ctgtcgtggc tgcgcgagaa
     2041 ggtccacgtg cacgcgtacc tccggccggc gaaggagatc ggcgaggaga tcatcggggg
     2101 cccgctgacg gcgcagccgc tcctcgacta cctggcgaac aagttcaggt cgctgtacgc
     2161 ctgagccagt ccgtcaggtc gagcccgcct tgacgcagca cgcggggctc gccgacgagc
     2221 gttaacaccg tcgagggaac gccgtcgcaa gtgccgccgt ccaggcagac gaggtccggc
     2281 gcgagggcgc acgcttcctc gtaggtcgta gccgggggtt cgccggagac gttggcgctc
     2341 gtgactgcca gtggcccggt ctgctccagg agtgctctcg cgatcgggtg cgcggggacc
     2401 cttactgcga ccgtcgccgg gtcgttgccg ccgaggtctg cgtcgaacga ttccttgcgt
     2461 cggactacca gcgtgagggg gcccggccag tgttcgtcgg ctagagccct cgcgctgtcg
     2521 tcgagctcgg cgatgccgcc gagctggacg acgtcggagc ccaggaccgg caacgccttg
     2581 tcccgcggac gctgtttgac gtcaaatatc gccgctgcag cctcggggcg cgcgccgatc
     2641 ccgtacacgg tgtcagtcgg gacgatcacc aacgctccgt ctcggatcgc gtcgacggcc
     2701 tcctctagcc cggccatcgc gcctccgcga tcctgtcccg gccgttgagg tccggctcga
     2761 tcgctacgtc ttcgtagccg cgggcggcga gcaacgcccg aacctggtca ccttgggtct
     2821 cgccgatctc gagcaagagc caaccgtccg ggcgcaacca ggccggggct tcgccggcga
     2881 cccgggcgat gacgctcgtc ccctcctccg gcgcgaacaa ggcctcgggc ggctcgtggt
     2941 cgacgacgtc acgaggcagc aacgaacgtt cgccgtggtc gacgtaagga gggttggaga
     3001 cgacgacgtc gacgcgtccg gcgagatcag cgggaagggc ggcgaacaag tcaccctgga
     3061 cgacctcgac gcgcaacccc aacgagtcgc gattggaggt cgcccactgc gaagcagtcg
     3121 gcgacagctc ggtcgcgaaa actcgcgcgt cgggccgctc gtccgcgacc gacagcgcga
     3181 tcgcgcccga gccggtgcag agatcgacga ggatgccttc ccgcgggagc cgcgccatcg
     3241 cacgctcggc gacgagctcg gtctccggcc gcgggatgaa cacgcccggc cccaccttca
     3301 gctcgagcct tctgaacgcc gcagtcccga ggacgtattg gagcggctcg cccgcgagcc
     3361 tccgccgcac gagctcgtcg acgtcgcggc ccccggcccc ctgctcgatc cagcgagcct
     3421 cggcctccgc cgactcgaca ccgccggccc gcaaccggcc tgcgattgcg cttggccgct
     3481 cgccgaggcg accgggcgcg ctcaggagcc gtcgtctccc aacgactcgg cccggtcgct
     3541 gcgcagcagc gcgtcgacga ggtcgtcgat gtctccctcg aggatctcgc cgaggcggtg
     3601 cgtcgtgtag ccgatgcggt ggtccgtgac gcggttgtcc gggaagttgt aggtcctgat
     3661 ccgctcggag cggtcccccg tacgcacctg cgacttgcgc tcttcggcgg cggccttctg
     3721 ctgctcctcc tgcttcgcct gcaggaggcg ggcgcgcagg atccgcatcg ccttcgcctt
     3781 gttctggagc tgcgacttct cgtcctggca cgcgacctcg acgcccgtgg gcaggtgcgt
     3841 gatcctcacg gccgagtccg tggtgttgac ggactggccg cccgggccgc tcgaccggta
     3901 cacgtcgatc ttgaggtcct tcgggtcgat ctcgacgtcg acctcctcgg cctcgggcag
     3961 gaccatcacg cccgctgccg acgtgtggat gcgcccgctc gactccgtca cgggaacacg
     4021 ctgcacgcgg tgcacgccgg cctcgtgctt gaggcgcgag tacgccccct tgcccttgac
     4081 ggcgaacgtg acctccttga acccgttcac gcccgtctcg ttcgaagtca gcacctcggt
     4141 cttccagcga cgggtctcgg cgtagcgcga gtacatccgg tacaggtcgg ctgcgaagag
     4201 ccccgcctcg tccccgccgg cccctgccct gatctcgacg atcacgtcct tgtcgtcgtt
     4261 ggggtccttc gggatgagcg cggcggcgat ctcctcggcg agccggtccc gctcgtcgct
     4321 ctgggtcgag atctcctcct cgaggtaggc gcgctcgtcc ggcgaggcgc ccgacagcat
     4381 ctccttggcc tgctcgaggt cgtcggccgc cgccttgtac cggtgccagg cgtcgaccgt
     4441 cggccggaga tctgaatgct ccttgccgag ggcccgcagc tcgtcggggc ggtccgcgag
     4501 gccggggtcg gagaggaggc gctcgatctc gccgaagcgc ttctccatct cgttcagtct
     4561 gtcctcgggc aacttcatgg gcgcgaaaag atacacctgg cctcgcacgg ccaacctgta
     4621 gtaggcatgg tcgggcaagg accgaggggg cattgcttga gcaccacgag gcgcacgttc
     4681 atccgcggga tcgccgcggc gactgccgtc gtcgccggca gggccgcgcc cgtgctcgct
     4741 cgcaggcgta aggcgaagcg aaagaagccc gagccgcagt ggctcttccg ggaccagttc
     4801 cagcgccgcg actcggccgg ctggggatgg ccctggttca accagcgcta cggcaggcgc
     4861 tgggcgatct cggaccgccg cggcgtctac cggctcccgg ccaccgagaa caacgtgtac
     4921 taccggccga acccgatctt cgtcctcgac cacgacgtcg cgaacgccga cctccgcgcc
     4981 acgatctcaa tgtcgaacga gcaggcgagg ttcggcatcg tcgctcgcgg caccggctac
     5041 gccgagtact acgcggccca catcgggccc ggcaaccaca tccggctcgt gcgctgcggg
     5101 caccacgacg agaaggtcct cgcgcgcaag cccttctacg tcgaggccaa ccggcgctac
     5161 cgcatccgct tccagatccg cggcgcggcc cccgtccgac tgaaggttaa ggcgtggccg
     5221 gtgaccgagc cggagccgcg cacgtggaac atcgacaccg ccgatccctc tccgggcgct
     5281 ctgacgacgc cgggaccgtt cggcctcttc gcggagcacg gcgtcgacgg gcgaggcgcg
     5341 gcgctgaggg tctcggactt cgtagcgtgg tcgcaggaga agccctcgta cacgcaagct
     5401 tcgatcacgt acgcgatggc ggggccgatc caggggtcgc gggtgaaggt cgtggcgaag
     5461 tgcgcgttcc ccgctcgcgt cggcttcgac tacggggccg atgcgacgtt ctccaacgtc
     5521 cgccgggtgt gggccgggaa cagctcggac cgcgcgcaga cggcctcggc gtggctcgac
     5581 ctcgagggat tcgacgccgg gtccgtcgtc cactggcggg cgtgggcgct gcgccgcggc
     5641 aagcgggtct tcggcccccc gtcgagcctg cgcatgcctc ctccgcccgg actgccggtc
     5701 aagttcgcgt tcggctcctg cacacgctgg aagccgtccc cgcgcccgtc ggtcgaccac
     5761 gcgcgtctgc agctgccgga cttctacctc catcagggag acctcggcta cgtgccgcac
     5821 cgcgtgatca cccacgcgcc ggacacgtat caggaccact ggacgcggat gttcatggac
     5881 ccctcgttcc aggcgatgac gcgctcggtc cccgtcggcg tctcgcagga cgacgccgac
     5941 tacgggacta acctcgccga ccgcggctcg ctccggcggt tcacgatccg cgcgcacgac
     6001 gagctgacgg cgaacccgcg cagcgcgtac tacacgttcc gctgcgggga catggcggtg
     6061 ttcgtgatcg actcgcgcag gtactccacc ggcaagaacg tctcgccggc gcggcgcacg
     6121 aagctggggg ccgagcagaa gcagtggctg ttcgacgaga tgacgaaggc ctccgaggac
     6181 gacgtcggcc tgtgcgtcgt cgcctcgccg caggcgttcg gctccgaccg gagcccggcg
     6241 tcgtggcgca agggcttcga gaacgagtgg cgcgagctga tggacttctt cgaggagctc
     6301 gtgacgccgg tgctgatcgt gtcgggcgac gcgcacggcc accggctgca cgagtacccg
     6361 cagaagaacc tcgacccgaa cgtcccgagg atcgtcgagc tcgtgtcgtc ggggaccgag
     6421 cagaacaagt tctccgacga cgtcgacccg cgcatcgtgc tccgtcaggc gaaggggtct
     6481 ggcttcgggt tcgtcgagct gggggccgag gagacgacgg ggggccagcg ggtccggacg
     6541 ctgacgctgt cggcgctgag gagcacggac ggggcgccgc tctgggcccc gcagcgctac
     6601 ctgatcgtga gaggcgtggg gatcctcccc ctgatcctct agccggtccg ccccggtccg
     6661 ggggcgtcac tgacgggtgc gcgacagcgt tacgccattc tcaacaaatt cgcccgatcg
     6721 aagtaaggaa tccgagcaac gcgtcgtatc ccccgccgta ggcggacgtg aaaagcgcgt
     6781 ccatgggcgg aaaggatgct ggggatgatc cttggcaaga acaagacgat cgcggctctc
     6841 gctgcaaccg cgatcgtcgc gtcgggggcg ttgttggcgc ccgcggcaca ggcagcgacg
     6901 aactgcctcc cggtcccggg ctcgacgctc gggtccgacg tgaaggtagg gcccgtcgaa
     6961 gagcgggtgc cggcgatctc ggacatcgtc gtgtgcgcag gcgggggcac cgtcccggtg
     7021 gcacgcgtcg cgacgagcgg cggcacttgc acgacgagct gcatcacggt ctacgtcggg
     7081 ggatcggccg tagacctcga cggcgcttcg atctcgtggt cgcaggacga cgtcccgcag
     7141 cccgaggtcg ggctcaaccc gggtcccgtc ggcaccgggg gcgaggtctg cgctctcagc
     7201 gtcggcttcc cggacgctcc ggacaccgat tgcacgatcg ctatcggccc ggacgacccc
     7261 accggtcacc tcgaggaggc gggcgagctc gtcgacaccg tggtcggcct ggtgacgacc
     7321 gcaggcgaga tcacctgcaa ccagatcccg gactggtacg accccgagac gggaagctac
     7381 gccgacttct gctcgcagcc cgacctgtgg gccactgccg tggcgaacca gggcggcgag
     7441 ctcgcttgcg gagccatccc ggtgatgtac gacgactggg gcaactcgta cgagttctgc
     7501 gacgacgcga ccggctggac gaccgcgttc atcgacgaca cctacgagac gtgcaactac
     7561 acgctcggcc cccagtacga cgagtactgg tgggactacg tgtacttctg cgacgacccg
     7621 atcaggtgga cgcagctcgc gctcgagaac ctgtgccgga acctctgcga cgtccagacg
     7681 atgcagaggc tcatcacgac catcagggag ctggcgaacg agaacgtgca gatccgcatc
     7741 ggctgagaac cggtgacgcc cgggcccaca ccgggtgacg gcaactcgaa cggccccgcg
     7801 ctcagcgcgg ggccgtatcg cgcccgagca aaaaaggaat ccgagcaccc cagcccgtac
     7861 tactcccccg gcgggcgtga tcggcgcgcc ctttggcgga agggagcatc cacttggact
     7921 tcgctcaacg acgagcgtgc gccgtccttc tgacggtgag catcgtggcc ctgggggccc
     7981 tctgggcacc tccggcgcag gcagcgacct gccagtccgt acctggctcg accacgggcc
     8041 tcgacctctc ggtcgcaggc cagcaacggc gcgtgcccgc catctcgggg atcaccgtgt
     8101 gcgtcgactc tccggtgatc cccctggtca ccgtgagcac cagcggcaac gggaactgca
     8161 ccgtcggctg cctgtcggtc ctctcctcgg gcggcgactt cgacacgggc gccgtcacgc
     8221 tttcctaccg ggaggacggg tcgacgaggt cccagaccgt cagcggcggg agcctcggcg
     8281 gcccggacgg atcgtgcctg ctcagcgtcg gtgcgcccga cgcgccctat ccgacgtgct
     8341 tcgtagcgct ggggatcgac gaccttcccg tcgatcccga tgagatacag gaggacctcg
     8401 accagctgca ggaggacctg gggcaggacg tggacgagct ccaggaggac ctcgaagacc
     8461 tccagggtga cctgggcgac caggtcgacc agctcgagga ggacctcggg aacctggagc
     8521 agacggcgtg cgacacgatc cccccgctcc aggacccgaa cagctgggag gagtaccgct
     8581 tctgcgacga cccggcagcg tgggccggcg tcatcacgga gggcgtcctg ctcttcatcc
     8641 gggacacggt ccggccgatc caggacctga tccgggaccg gccgacgccg caagagatcg
     8701 ctgaaatcgt ctgcgacgca ctgctcgagc cgtcctgcta gcaacgcgtc gaagcggaaa
     8761 gggcgccgcc ctcctctgac gggggcggcg cctcttcttg cgggctctag gcgaccggcg
     8821 agtcggggat cgtcccggcc cccgcgggct tctctacgtc ctccgccacc gcgttcatcg
     8881 ccgctatcgc cacctcgatc tggtccagcg acggcggctt cgtcgtgatg cgctggagcg
     8941 ccaggccggg cagcatcgag atcctgaaga acagcgagtt ctcgttcttc gacgccagct
     9001 tgatggcttc gtaggagatg ccggcgagca ccgggatcgc cgcgatgcgg atgccgaccc
     9061 ggggcagcag gcccaggtgg gcgaacaaga ggtcgatcac gaagtgggcg atgatcgtca
     9121 ggaacaggac gatgaacagg aagttcgtcc cgcagcgcac gtggagcgtc gggtacttgt
     9181 cgtcgatcag cttcgggtcg agcgggtcac cgttctcgta cgcgtagatc gtcttgtgct
     9241 cggccccgtg gtactggaag acccggcgca cgtctttgaa cgtcccgatg acgagcaggt
     9301 agccgacgaa catcgacagc ctgatcccgc cttcgatgag gttcgctacc agcgggttgt
     9361 cctcggagag gttgccggtc aggttctcga tcagccgcgt cccgaagacc ggcagcgcga
     9421 tgaagatcgc cgagaacaac agcatcgcgg tcccgagcgt ccagccgagc tgcttgtcgg
     9481 agagctgctc ttcctcctcg agcgcctggt tggccgagat catcagcgcc ttgatcccga
     9541 tcgcgaggga ctcccccagc acgaggaccc cgcgggccag cggccacttc agccacgggt
     9601 ggcgctcggc gacgctcggg agcgggtgct tctcgacgga gatcgtctcg tcgggacggc
     9661 ggcatgcgac ggaccacgac gccggccccc gcatcatcac gccctcgagg accgcctgtc
     9721 cgccgatcga aggccgcggg gtcggattct cttcgctcat gcggggagtg tagtgactgc
     9781 tactccgtcg cgcttgcgtc cgccgtcgcc ggagcatcgg ccgcgggctc ggcccccttt
     9841 tcgttctgcg gtccggccga ggccgccccg cccggcttgg cgcggttcgc gtacttcttc
     9901 tggaagcgct cgacgcggcc cccggtgtcc acgagcttct gcttgcccgt gtagaagggg
     9961 tggcactggt tgcagagctc gacgtggagc tcggccttca cagaacgagt cgtgaaggtg
    10021 ttgccgcacg agcacgtgac cttggtctcg atgtaatcgg ggtggattcc ctgcttcacg
    10081 gtccctcatc ctctccggct tcagttgcct gcatcgcctc gcgtcgctgc ctggccttct
    10141 cgtatgcgac cgaggcacgg gccatgacct tgaaagtcag gagcaacccc ccgaccaggc
    10201 cgatgataag cagccattgc ggccaaccca agcccaggag caactattcc cccttgaccg
    10261 gggccttcgc tatctcccgc aagaactgag cgttgcccat cgtggccttc atcttgtcga
    10321 tgaggagctc gagagcgccg ccgtccgcga gtgcgtggag gaccctccgc agccgccaga
    10381 cgagctggag ctcctcgggc gtgagcagca gctcctcctt gcgggtgccg gacgcctgga
    10441 tgtcgatcgc cgggaagatc cgcttctcgg acatccttcg gtcgagcttc agctcccagt
    10501 tccccgtgcc cttgaactcc tcgaagatga cctcgtccat ccgggacccg gtctcgacga
    10561 gcgccgtggc gaggatcgtc agcgatccgc cgaactcgat gttgcgggcg gcgccgaaga
    10621 acttcttggg cgggtagaga gccgtcgagt cgacaccacc ggagaggatg cggccgctgg
    10681 ccggcgtcgc gaggttgtac gcacgcgaga gccgcgtgat gccgtcgagc aggatcatca
    10741 cgtccttgcc catctcgacg agccgcttgg cgcgctcgag cgtgagctcg gtgacctgga
    10801 tgtggtcgtc ggagggccgg tcgaacgtcg agtacacgac ctcgccgtcc accgagcgct
    10861 gcatgtccgt gacctcttcg gggcgctcgt cgacgagcag gacgatgagg tgcgtctcgg
    10921 ggttgttcgc ccggaccgcg ttcgcgatct ccttcatgac cgtcgtcttg ccggccttcg
    10981 gagggctcac gatcatcccg cgctggccct tgccgatcgg tgcgatcagg tcgacgatcc
    11041 gcggcgtgat cgagcgcggg ttgtcgggct gctcgagcgt caaccggtcc tgcggataca
    11101 gcggcgtgag gtcggcgaaa cggacccggt tctggacctc ctccggcggg cgaccgttga
    11161 tggtcttgat cttgaggagg gcctggtact tctcggactc cttcggggag cgcacggagc
    11221 cttcgacctc gtcgcccttg cgaaggtgga agcgccgcac ctgcgacacc gacacgtaga
    11281 cgtccttgtc gccgggcagg taaccgctcg tccgcaggaa gccgtagccc tcgggaagca
    11341 ggtccagcac gcccgtgcgc acctctgcat tcgggtcgtc ctcggcctcg agctcgtccc
    11401 agcgctcgtc gcgcatgtag tgcgggacgc cgggagcacc gccgccgcga ccgcgccgcc
    11461 tgcgccgtcc gctgccgccg cggtcgttgc cgccgcggtc gttgccgcca ccgcggttgt
    11521 cgcggccgtc gcgcggctcg gccttctcgc gcgggcgtga cctgttctcg cggccctggg
    11581 gacgccggcg ctgctggcgg tcgtcgttgc gacggtcgcg gtcgtcgacc gcgccctcgt
    11641 cgcgcaccgc ggccggagaa gtcccgccgt ccgacgacga accctggtcg cccgagggag
    11701 tcgaaggctg cgcgggtgcg gcggcctgcg cgcctgccgg cgcgggagcg ggcgcgtcac
    11761 caatcggtgc ggcgacggga gccttctcgc gcggcttgcg cgcaggtcgc ggcgcggccg
    11821 aaccaccgtt cgaccccccg cccgcgatcc ggtcgacgat ctcggccttc ttcaagccgg
    11881 cgaccttgag gtccatcgac ttggcgatct gcttgagctc ggcgaccgcc ttggactcga
    11941 gtgccgcgcg gtccatcgtc tctgccactt ttctttccta cccctcttgc atcgagggag
    12001 gaagctcacc catatggggt ttcgcctgct cccagtcgtc ggtgaatcgc ttgaaccctg
    12061 aatccgtcag cggatgcctc acgagttgtt cgaagacctt gaaaggcatc gtcgctatgt
    12121 ccgccccgag gcgagccgcc tccgttacgt ggatggggtg cctgaggctc gccgctagaa
    12181 cctgggtgtc gtagccctgg acgatgaaga tctcgcagat gtccgcgatc agccggagcc
    12241 cgtccgtggc gacgtcgtca agcctaccca caaacggcga cacgatgtaa gcccctgctt
    12301 cggcggccaa aatcgcctgg gccggcgtga acgcgagcgt gacgttgacc ttgatcccgt
    12361 cgttcgcgag ttgccgcgtc gcggccaggc cgttcggcgt gagcgcgatc ttcacgaccg
    12421 cgttgtccgc catcttggcg atctcgtgac cctgcctgac gagcaagtcg gtgtcgtccg
    12481 aggccgtctc gatcgagacg tcgccgtcca cgagcgacag gatgtcgcgc acgagcggga
    12541 ggaacccctg cttctccttc gcgatcaggc tggggttcgt cgtcacaccg cgcaggacgc
    12601 cccaccggac tccctcccgg atctcgtcca ggttcgcggt gtcaagaaac aacttcacgc
    12661 gtgcctcctt ctgcatcttc cgtcgccggc ggtctgccgc gtccgacggc ttcgccggcc
    12721 ttctcgatga gcttctcgtc cgttccgccg acgtccacgc ccttctcgac ggactcgagc
    12781 gccttgttca cgatgtggtc gatgacctcg gacagcgtcg cgtcgaggtc gtagtgcgcg
    12841 atcgcaggca cgccgcgcat caacgcggac gacaccatgt acttctgcag cttgcggatc
    12901 ttgcggaacg cgttgagata gcgctcgccg ccgcgcgagc tcgtgtcgcg tcctctgcgg
    12961 aagaagtggc tgcggtggag gtcctcgtcg tcgatcgtta tgagcagcgg cacgacgatg
    13021 gccttgtcgg actcgacgct ctgcaggaag ccggggacga ggtgcgcgcc ctcgaggatc
    13081 atgtcggtgc cttcctggat ggcgcggtcg atgagcgcct gcgcacccac cgcgaccgcg
    13141 gcagcctgct cgcggaagcc gatgatcagc ttgtcgccgg agtgcgggat cggctggcgt
    13201 acggcgctgt cggcttcgaa cgacgacgcg tagagcgtgg ggaacatcgc ctcggtcagc
    13261 gcgctgcgca gcacctcgcg caccgcgtcc gtggagacga cgcgggtgat gccgaggcgg
    13321 ctcgccagct gcgtcgcgag cgtcgacttg ccgacaccgg tcgccccgcc gagcaagatg
    13381 atcaacggga cgtcgagctc ggcgaccgac tgccacttca cgaaggcctg cgcgtaggag
    13441 tcgcccgcct cgcgccgcag cacgcgcacg gtgaggtcgc gcagcgcttc ctcggtcacg
    13501 taggacgacc ccgcgccgcg cagctcctcc tcgaccttgt tcgccaccgc gtaggcacgg
    13561 ccggggccga ggccggtcgc catgagggaa gacgccgtca gacccttgga gtacgggagg
    13621 ccgcgttcgc tctcggtgat gaagatgtgc ttacgtcgct tctcgttcac cttctcggtg
    13681 ccttcctgcc gcgaggtccg cagccgtcac gagcatggcc tcgaggtcgc cgtcgtcgat
    13741 cgggacgggg acgttgtcca tgtagacgac gtccatcccg gaggagaggg cttccctcgt
    13801 cgccacgtcg atgccggccg ccttgatctc gcacaacagg acgtcggcct gtccggcgtc
    13861 cttcagctct tgcttcaact tccccctgtc ggacagcgag tggctgatcc cgaccacccg
    13921 gcacccgtgc tcgccctcga gatgacgctt gatggagtcc cctgctgcct caggggccgt
    13981 agtcgcgacg accacctctg ctccttgcac cgagcgagtg ggggcgggac ggaagacggt
    14041 tcgaaccagc tcgatctctt gcttgggtcc cggcgaccac gaatctctta cgagagaggt
    14101 tgtacgggag acctgagagg gagaaccgaa cggctcttcg cacatagtaa cgaccacgaa
    14161 gtcggcaagc aacaaccggt aggggccgag gtatccggcg aggtactcct cggggatcga
    14221 cgccggaacg acgagcaagc tcacgtcggc gtggcacggc ggtatggaag accccgatcc
    14281 ctcgagcagc aggaggtcgc ccggcagctc gttggcgagc tcgatcccgg cgggcacgtt
    14341 cgagatctcg acgccgccgg cgaggccccc gccgcaacgc ctgcaaccca cggtcgggac
    14401 tcgccccagc agcgcgtcct cgacgtagtc ggacgccgcg tgcttgccgg cgtcggccat
    14461 ctcgaggagg tccttcggct cgaggtcgat ctcgtcgccg cggagcacct cgggctcggc
    14521 gggacccccg cgccccatgg cgacgatcac cggatgccgg ccgctcgagc tgagcacgcg
    14581 ggccgtgaac cccgcgaccg cggtcttgcc ggtccgcttg cccgtcccga tgacggcgag
    14641 cgacggcttg gaagcgagtg gcggacgccg ggggggccgc agctcgaagt ccgccccttg
    14701 gtagggcgtg tcgtgccaca gcgcgatcga ggccagcacg tgcctgcggc ggtagtcgag
    14761 gaccggctcg tccgagaggt ccacgaccgc gtcgacgtcg ttgtctcgca ggaactcgtc
    14821 cagggccacc cgggggtcct cgccgaggac gacccggacg tcgccgtacg cctcgacgcc
    14881 ctccgcgggg agcttctccc ggcccccgac gagcagcgcc gcgaccacct cgtctccccc
    14941 ggccgccagc tcgtcgagcg ccgactcgac caccggcgga tagtgctcgc cgtcgaccac
    15001 ggctaggtaa cggatggctg cctcctcgat ggctccggcg gcggggttgg aaggaaaagg
    15061 ttacggagcg tccccaagtg ggaaccctat gtgacataac cccgcgactt tggaggtcac
    15121 gatgttcggt cgcactagag acgaagcccg ccacgaggac cgccgcgtca ccggtcacga
    15181 ggtggtcgcc gcacgcgggg gcgtctcccc cggcgcgatc ctgacgggag tcgtcgtcgc
    15241 ggtcggcgcg atgttcgtgc tcaccgccgt gagcgccggc atcctcgtcg gcctcggcgt
    15301 cgaggacgag ctgacgcccg gccaggccac cgacgtcggg atcgccaccg ggatcgggct
    15361 ggtcgtcgcc caattcctct cttacatgtg gggcggctac gcagc
//
LOCUS       DASQDO010000289         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_153239, whole genome shotgun sequence.
ACCESSION   DASQDO010000289 DASQDO010000000
VERSION     DASQDO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153239"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VEV43_08125"
     CDS             <1..323
                     /locus_tag="VEV43_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005169380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYP23525.1"
                     /translation="TENAPADGVLEAAGLRLDPERFEVTVGGEVVHMPRKEFELLELL
                     MGNAGRVLTRETLIDRVWGSDYFGDTRTLDVHVKRLRSKCEPDPHNPEHLITVRGLGY
                     TFVP"
     gene            complement(433..1317)
                     /locus_tag="VEV43_08130"
     CDS             complement(433..1317)
                     /locus_tag="VEV43_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23526.1"
                     /translation="MRRTLAVTIAAALLVGGAVAGTGHAKARRVKVERHDSAGYVGAK
                     GSAGAEPTIYVDEGIFETERYERDVSVTVIDRTGQAVAGLVRQDADGDGTWEIEEAFC
                     GATAEPVAIEGGAPVVVKVQPGPCADGTPAVMTSGSIRVAFTGFTKAPAVPAPSLGCA
                     PTKAAREITEKYVLASGAGAATEAYPVGFFELADDEAIGGAQFVAGCGEGRLEVTLTD
                     QTGLPTRAAIAVDPDGVGGDAPQDFVAEVCGTTESPVAITPGAEVTIFVVEGPCSDGT
                     PATATTGTVTATLTAPAK"
     gene            complement(1409..1618)
                     /locus_tag="VEV43_08135"
     CDS             complement(1409..1618)
                     /locus_tag="VEV43_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP23527.1"
                     /translation="MTQAPWTDETDETIARLVVEGLPNKAIAARLGIPEGTVKWRLHR
                     MYGRAGVQSRTRFVLVLRDLATGDG"
BASE COUNT          267 a          675 c          643 g          273 t
ORIGIN      
        1 cgaccgagaa cgccccggcg gacggagtgc tcgaggccgc cgggctccgg ctcgaccccg
       61 agaggttcga ggtcacagtc ggcggcgagg tcgtgcacat gccgcgcaag gagttcgagc
      121 tgctcgagct cctgatgggg aacgcggggc gcgtgctcac acgggagacg ctgatcgacc
      181 gggtgtgggg cagcgactac ttcggcgaca cgcggacgct cgacgtgcac gtcaagaggc
      241 tccgctcgaa gtgcgagccg gacccgcaca accccgagca cctgatcacg gtgcgggggc
      301 tcggttacac gttcgtgccc taacctcccc caccgacggc ggggggccgt ccggtggcgt
      361 gaggacgcca gtgatccatg tggcgggacc acggcggagg gatgaaccga acgtgcacta
      421 cacggggtcg ccttacttcg ccggggcggt gagcgtcgcc gtcaccgtgc ccgtcgtcgc
      481 ggtcgccggc gtcccgtcgg agcaggggcc ctcgacgaca aagatcgtca cctcggcccc
      541 cggcgtgatc gcaaccgggc tctcggtcgt gccgcatacc tcggcgacga agtcctgcgg
      601 cgcgtcgccg ccgacgccgt cgggatcgac cgcgatcgcg gcccgcgtcg ggagccccgt
      661 ctggtcggtc agcgtcacct cgagccgccc ctcgccgcac cccgcgacga actgcgcgcc
      721 gccgatcgct tcgtcgtcgg caagctcgaa gaacccgacg gggtaggcct ccgtggcggc
      781 tccggcaccc gacgcgagca cgtacttctc ggtgatctca cgcgcggcct tggtaggtgc
      841 acaccccagc gacggcgccg ggaccgcggg ggccttcgtg aaccccgtga acgcgacccg
      901 gatggagccg gaggtcatca ccgcgggagt gccgtcggcg cagggtccgg gctgcacctt
      961 cacgacgacg ggggcaccgc cctcgatcgc gacgggctcg gcggtcgcgc cgcagaacgc
     1021 ctcctcgatc tcccaggtgc cgtcgccgtc cgcatcctgg cgaacgagac cggcgacggc
     1081 ctgtcccgtc cggtcgatca ccgtgaccga cacgtcgcgc tcgtagcgct ccgtctcgaa
     1141 gatgccctcg tcgacgtaga tcgtcggctc ggcccccgcc gaacccttcg cgccgacgta
     1201 gccggcggag tcatgccgct cgaccttcac gcggcgggcc ttcgcgtgtc cggtgccggc
     1261 gaccgcgccg ccgaccaaca acgccgctgc gatcgtcact gccagtgttc gcctcatcgt
     1321 ttcgttcctt tcgttcgatc cgtctgcttg cccgtcttgt ttgcgtcgcc gggtcaccga
     1381 cctgccccgg ccaacgaagg ttggcggctc agccgtcgcc ggtggcgagg tctcgcagga
     1441 cgaggacgaa gcgcgttcgc gactggactc ccgcgcggcc gtacatgcgg tggagccgcc
     1501 acttcaccgt gccctcgggg atcccgagcc gcgcggcgat ggccttgttc ggcagcccct
     1561 ccaccacgag gcgcgcgatc gtctcgtccg tttcgtccgt ccagggagcc tgcgtcatcg
     1621 ggtcttccac gtcgcggagg aaggaaaagt cacggcgtta ccgaacgacc ccttcggacg
     1681 tggagaggac atggagcgcg tgaacgaaac acccagcggg cggtccgccg ccttcggcga
     1741 ggcgtgggcc gagctcgagc ccgagctccg gcgcagcctc gcttcggccc ccgccaaccg
     1801 cggagacgac gccttgcagg agacgtcgag aaagctgcgg agggtctggg actcgatc
//
LOCUS       DASQDO010000290        22711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_887572, whole genome shotgun sequence.
ACCESSION   DASQDO010000290 DASQDO010000000
VERSION     DASQDO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22711
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887572"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VEV43_08140"
     CDS             <1..382
                     /locus_tag="VEV43_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23528.1"
                     /translation="RAEREFVMQRYIDKNIQARRLAPGARGAINDLAEHGATLVMDDA
                     PAKAAGKLLPMFGVPFLPEGVGTAYLKRIAPFVAAKNASPAQVDAMYRQMVELVHSEG
                     GFAQHAVVVTSDSRYLGEIPFASW"
     gene            complement(349..1392)
                     /locus_tag="VEV43_08145"
     CDS             complement(349..1392)
                     /locus_tag="VEV43_08145"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HYP23529.1"
                     /translation="MPTFDEDGSIPSLIRTFLSESPEISSGDVARTASVTRQAAHYHL
                     RRMVEAGELKAIGAGRGRRYALAAAWRKQFETAGLLEDAVWREVLLEVPSVGGMPERA
                     QDIARFAFTEMLNNAIEHSGADIVSISVLPGDAQGFVVSDEGIGAFENVRRAKRLEDH
                     LAAIQEIAKGKLTTDPSRHTGQGIFFTSKAVDLFTLTTNGWRWTVDNVRHDEAIARVG
                     ARRGTHVSFSVDPHTLRSLNDVFDDYTDPDTLDFDTTRTVVRLFEYGVSFVSRSEAKR
                     LTRNLDQFKEVFVDFRGVEWVGQGFADEVFRVWQSDHPDIRLVPVNMNEAVRRFVERA
                     RPPYQEAKGISPR"
     gene            complement(1624..2436)
                     /locus_tag="VEV43_08150"
     CDS             complement(1624..2436)
                     /locus_tag="VEV43_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYP23530.1"
                     /translation="MTRGKRAAAVVVLLLVVGGVGAGAVWQIGELEAQIDDIEVLLEE
                     DQSADASAAAAVARQVGELEDRFDPLYSRVNELSEGLPPNVPDIVARARESIVTIELT
                     NGVLGSGFAVNLQPDPGYTAVVATNHHVIKDALRPNGPEVYVNHDGTRYPAEVTAWDK
                     GNDLAVVDVAADIPALEWAIEHDHEPSVGDFVVAIGSPSGLAGTSTTGVVSQLYPNYV
                     QIDAAINPGNSGGPLLNKFGEVLGVNTFKLQGENLNFAVRVEVLCEEIVTDC"
     gene            complement(2447..3727)
                     /locus_tag="VEV43_08155"
     CDS             complement(2447..3727)
                     /locus_tag="VEV43_08155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYP23531.1"
                     /translation="MAVSAALVGSSLGAPKGPRPPKPAPGWGAPVLVHADQAHRETSL
                     AINPVNEKVMLACAPSGVPNTQNNQSYFHRSRNGGRTWEPLRVENDDAGEGRTYTFEG
                     GDCDVAFDDGGTMYSADTWLGNLSVGHSRDNGGTWEGTPVAASGLIVDRPWLVGGPEG
                     TIHVSYQDLQCCMPSAIWYTKSTDYGMTFQPTSMVAGAGAEGAFTWQGNLVVSEDGND
                     LYLPYTRRAGAALGSLDDQGPEKLFVAASHDAGATWTSNLVAAMPNPMSYLYPSLALD
                     RTGKLHVVFSSMTDKDRPIWYSTSTDDAATWTKSVPLTRGDAGFSPWIAVDDAGNAAV
                     VWYGSPDPATNETTESDWYFYWARIPAGKFSAKNVRSGTTTREPLFTGVSPIPEFENV
                     RLDSKGNIHIGASAYRAGENGAGPGWAIYYQRER"
     gene            complement(3880..5604)
                     /locus_tag="VEV43_08160"
     CDS             complement(3880..5604)
                     /locus_tag="VEV43_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23532.1"
                     /translation="MRRYLTGLLSGVLTGALALGAAWATTGLVSVETPDVTPSLVQPS
                     PSTSASPQATPAPAPSPAASPAGGRLAGGTFLVGAHNESIAPVPQSEGGPWNPHGGGY
                     DCGPMGYEYLPNSDPSCLRTFDRAWATGVDAASDLGIYARAMAIGNGEDTLVFVVLDT
                     VSWFYGYDPSICPDDADAAQPQDTCGTRAIVVDLSDELGIPVENFVIASTHTHASADT
                     TARGPSWYYEFVRDRIKDSVRGAVSKMEPATLQTGAIPAKAFNVDRRIVTRAVPDAEL
                     TWLRAVALPSTDPATKGKAARTIATLVSFSAHPTVTAGNEDMHSGFVGHLAERLGQLW
                     GGSTVFVPGGLGDQTVHRSFGRRGIGYGLAELVFESARYGYTLRSNDIAAEQRILTIP
                     ADNASLVALNKAGIFMRDTTIPGPHAAGPSQSVQQKGRSRTPSCAGAGPLSVKSPVGG
                     FRIGTPGPSQDDRGFVPGDRGDAVVLMQAPGEIFASISLTTKDYLSRARNVMVLGMAN
                     DHIGYIIPAEQYDVRGANAAGIAQPSLEMTNYEESLSTGRCTGDQIQNALVEIGANLD
                     VLGVGEGR"
     gene            5721..6974
                     /locus_tag="VEV43_08165"
     CDS             5721..6974
                     /locus_tag="VEV43_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HYP23533.1"
                     /translation="MTSKRVSTKVMLAALMVAGSLLPSIPAGAQDGSAAKCVDGMAGI
                     YPCKAMDLLSHRTFSAAEGGGVSDVWGWADPETGNEYAILAGGGGVRFLDVTDPAAPV
                     YLGLMPTKGDGQTLWQEIEILNDHAYVVCDAIPCGLQIFDLKRLTGVEAAVPVWRPDV
                     VLPIGTFHSIASNPETNHIFLNGIGAVVGSPTGTPLIFDVSQPLAPVPVGGMLDDGYT
                     HDSLCPIYKGPDKRFKGNEICFNFNEDTITTYDVTANPQQPVQLSKVTYDNADYVHSG
                     ALTKDHAYLISTDEGDEVEYLIPSTLYIWDVRKLDKPKLIGTFVGKTLSIDHNVYSEQ
                     DALYHANYNNGFRILDMANASKGKLKEVAWIDTMPAADSADYDGAWAAYPYLPSGNIL
                     VGDMNGGLFVVRPHAKIYRSLGVKR"
     gene            complement(7037..7648)
                     /locus_tag="VEV43_08170"
     CDS             complement(7037..7648)
                     /locus_tag="VEV43_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23534.1"
                     /translation="MNARFIRSRAARGVLCSLTALALMTTAATAQVAVETPEEEDVLD
                     TVFVPDPGPIFLIDPAIVDQIVMGNAGELPNDWPHIVYLFYVLSTLTNGNCDNGATAQ
                     VPFKQGAKIKARGGMVCPTIQKEIALKVCIDFRQAGEWALLGCKPKAVTDTDRAHKRI
                     TVACQPGRFFYRTRTVATATNQADQESFDTATAVTSGRIACAV"
     gene            7824..9248
                     /locus_tag="VEV43_08175"
     CDS             7824..9248
                     /locus_tag="VEV43_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23535.1"
                     /translation="MPRHTGHMLHICIAASIAALAPAHAQPLPGEEPACFDKEATLVG
                     TEDADRLKGTERADVIAGLGGDDTIRGLGGNDALCGGPGLDVVNGAGGADLIAGDEGS
                     DLLIGGDGDDRLRGGAGIDFAEPNAGDDFVEGGPGFDGISLGSARRGVYVNLRKGIAR
                     GDGRDRVAGVSDIEGSRFDDRLVGPYGEGQILALDGDDRLVGGSHDGIFIPGPGDDVV
                     IGGRGGGDLIAFPFAEAAVVVDLRAGRARGEGRDSISRLDDVVGTDRADRIFGDPGRN
                     RLFGAPGFDVLRGRGGRDEAGYLFDNTRVSADLERGRAVSSGTDELYSIEDLSGSLRP
                     DTLSGDDRRNRLVGLAGADVLEGRDGRDELDGGDGPDQAFGAGGADRVRGGAGEDVVG
                     AGPGDDMLFGGEGADELDGNAGSDTADGGPDLDSCVNVEEQQNCEVPIYARSGPLALG
                     LRPGIPAWIGSRPLRLLWWLRLTR"
     gene            complement(9279..9461)
                     /locus_tag="VEV43_08180"
     CDS             complement(9279..9461)
                     /locus_tag="VEV43_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23536.1"
                     /translation="MMRYAKREVGAGLLVIGVAGVLEYAEVFGDGGSAFQLIAGIVFA
                     IVGFVILVRNAVGRSD"
     gene            complement(9469..9813)
                     /locus_tag="VEV43_08185"
     CDS             complement(9469..9813)
                     /locus_tag="VEV43_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23537.1"
                     /translation="MAGNVGAMPAQERSTGWTLFLGSAAAAAGIAGAFVYRFLTKGES
                     PDPAVQRQNRTRNTGLAVTFGIAALFFVRRAADAPRLVAMGLAFGSLTVTMLGYAFHV
                     LRTRDDGPAGGG"
     gene            complement(9920..18877)
                     /locus_tag="VEV43_08190"
     CDS             complement(9920..18877)
                     /locus_tag="VEV43_08190"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYP23538.1"
                     /translation="MNKRWITVRSIGIFMVTALLAQLPFLPAGAQPDPGPAREQNADG
                     GREEIERLRTATSKTYRNDDGTKTVQMFSGAVHFLDARGNWREIDTALEDATHEGYAW
                     QNGANAYRASFRRHTTDGFMRFAIGEQVFEITADGASPSATAMSAREHREKERGGGAS
                     PEVTPAPATPEPATEAPATEPPATEAPAEEPRGEKPSEPGAPKEKGAEGDVAATSAEE
                     PAGPSPEPSAAPSPEASPTETATPEPTPEPEPGDDLPGKSSLIYDDVFPGVDVEYRLL
                     PDGVKETLILDDASAPARYRFYLDALRDVPVEVEELDNGGWAFYVAPKPEPAFVLAPP
                     YAYDSGPSSRSRDGHASLEVRKVGGRYAVDLSVDAQWLHDQERQFPVYLDPTITIQPP
                     SLDANFIANCSGCTPWDYETIWVGTDSDTWRGAYKFDLSSIPVGANVTDAQFKSYYTG
                     ECVYTGSYTCGSTSHLINAHKITSNWSLTTTTSGLTFDPTTLSSFTVPASTAAQWMNW
                     GVTQTVKDWLAGTSPNYGIMLKRATEPANASGPDMPSRQYFETTIRPKLEVTYASAAV
                     TLLEPETLHSNGAELEWTKFNGTGFTSYEVHRGTTATFAPSASTLLTSIGDVATTSFV
                     DATAAPSKSFSYKVVAGGATSNGVTVTLPADGQSKKILQPVPTDGVATSGAKYSSYAS
                     CANYGKEPSLYFGTTSTSVWRSALMFDMRDVPAHATVNSADLSLWHPTMVTGDHTINA
                     HRVTSPWKEGSGVSTCTGDGATWYETQGGVKWGAQGGDIDGTPVDSERDWDEPAETDV
                     FNLAGVAQKWVSGSVPNYGIILKRSDETLVDGRRFTYYADDQSVSPTLRPKLVMVYTD
                     GNKAKGPAISVASPLKDTQVSGSAVKIVANASDDRRVDKVEFLVDGALVSTDTAAPWE
                     ATWNSTTVVNGTRNLTVRATDDAGNVTTSAAVPVVVGNSAAPTTSISAPALNATVSGT
                     AVTVSANAADDLGVSKVEFFSDGLKFAEDTASPYSVAWNTLDPANPAYDGVHTLTSRV
                     TDSHGQTTTSAGRNVTVSNAGTTKYKAGFTSTAVPQAMSYDPAAVTQTSYPVDVTVKN
                     NSTVSWTGTDLQLRYRWYNPATPSTFVDGTAVAFGTNLAAAGTRTLRVQVQPPPLAEG
                     IDRAQYQLRFDLQEKSTSAYFTAKGNKPLEHPVLINKALKATALGLERYYHYTGESVG
                     AGMGNLVNVANGNSILRWTPFMAPGRGLATVMDITYNSLEDKSDSPIGNNFSLSISSL
                     SRFGLPLDIHPNNADTIAGRSNKWIEFTDGDGTTHRFTQNAGGGWDEPPGVHLFLRQY
                     STTDLTKKWALTKPDRTTFFYDSEGYPTSVEDRNGNKIVFTLETVPPGEDPGGFKKRI
                     TKVTDAAGALNAAPNRSFNIDYYSKAEAHKPHVRGKIQTITDHDGSALDFEYYDDGNL
                     LRLTQRGGTNADGTALPSRTFVFTYTTSDGSGPAIPLAADRVNPASKTPNQSNRLFSV
                     RDARGAETTFTYYGPGSSLDRWKLKSRTNRTGNTTSFAYDNTLTLTTVTAPLSRVSKY
                     DYDSNGRVVQLTNPKNEVTNVAWTPDFHVSKVTEPNGANRQYTYNANGYLTDVKVMDR
                     NNVQITATQLVYNNLAVKAEGGAADTRDALAYWKAGRTIPHVSQLERRIDGRGNAWVF
                     QSDANGNITKVIEPEPAGLPDGSRYSTTYAWNTGTVDPGTISSITDARSKVTSFPTYD
                     ANGLATKIVDAKGNTTKFGYTDDGLLEWVQDPEHLQYEVTGHTDAQARDYRTYFYYDS
                     FHRLGQQSSPKSTQHDRGNLVWSAAKFDPNDNLVTQFTPAFGAGNPGLGAPTTMTYNA
                     MDRRLSRTGPESDRTEWEYDAANRVTKVIRPNGTATATVANDFATIYAYDALDRVVTQ
                     TQHNVRPDGTVIESLHTHACYDNAGNLVSVTSPRANKATITCGATGSAEPFTTKYTYD
                     DAHRRLTVTDPLNHVRKMAYDANSNVISTTNESNHVSTRDYDARNLLTRVIQPYEEGV
                     GRKVKTAYRYDAVGNKVKVISPRAWDASPDAEPNKQNFSRYVTTIVYDDVNRVERVEL
                     PFSNVLPEETGFQQQYVHKKYDKNGRLVRTSLPVTSLAPPADAHESWTVVTHWDPGWV
                     KTSKTAPNPIVHFDYEAPGWQKSRIPEMKDSPDTKNMERAMSWTYYPDGTLKTRTDQD
                     GGGVSYEYDLDNNVKSIVDDSGISGNEKRVEMKATYDSLDRRIEIRHRKAVDVSDTTT
                     NYTATEYAYDLNGNLIRRVDDATAVPGSTTLTGGKTSTFTYDAANWLDVQIDKGKTPN
                     DCTDDRKIDNDFTVTGWEQQRANHKALGTCTTDPVNGWQLKQQTNWTYFQNGKLDTLI
                     TKQRDDATATMKVVESHDLSYLSDQGFYVNGHRTKDVYSMDGPDTTAKCRPASPCTRK
                     FVYDPRDRLVKEDDGHSGVTSYTLDPAGNVTREAKGTATIDYSYTANQLTQVQAGSNV
                     NKYFYDDYGNLDCVTGATGVDTDCSKSAQGSPATGVLSDYAYDDLNRMTNYRSFASDD
                     NTNTDYSYDGLDRVIKEVEDHGSGATTHDRTTAFSYQGLTGLVTQEQITGTTAETKSY
                     SYDAYGNRVSMQHQKGTTTDNYNYAYDVHGSVSMLLKEAGGARASYGYTAYGKSDAAV
                     SANDENAKAPLNPFRFTADRFDSGSGSIDMGARRFGPDVGRFLQFDVFAGSLSDLSLS
                     IDPLTQNRYSFAGGNPISFVEFDGHMVIADGGGGAASDPNPTGDSGDGSSDGDEGFRL
                     SKTDVLQGVGEVAEEATNPRNWGPLADQIRGFGDDADDWLDNYVPERYADGFENTTDA
                     ISDGMHRHSDLAGKASKYLGWAGTAGDVLEFGGKFKGYYDNSGGDWGEAATRTGLEFG
                     GSLAGSAAVTAACAAAGVATAGIGAVACVGGAWLASEAGEYLGKELGEWWYEEGGQEM
                     VSDAWNATTDAVGDAVDAGQEFLGDAADVASDVGDTLTFWD"
     gene            19211..19930
                     /locus_tag="VEV43_08195"
     CDS             19211..19930
                     /locus_tag="VEV43_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23539.1"
                     /translation="MKLNRKQFIAVALAGACIASTGSYAASAQDATLDPAVDPEVVAE
                     SLSGELATPPPVQIAVDPAPVVIEEPAVSSPEEENLTNVTVRDPGPVMPADPGAVDSA
                     VVGSVGAAPDATSLAAVSTNVADNAGSGECSATASVPYKQDIKIWGNGSVSCGSVQRS
                     LNVIVCLEVRQAGAWDELGCKPKTVSQAKSTSKKVSVACVPGRFLYRTVVWGAATSRS
                     GNRSSIPQDTAVTSGRIPCAS"
     gene            complement(20004..20696)
                     /locus_tag="VEV43_08200"
     CDS             complement(20004..20696)
                     /locus_tag="VEV43_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HYP23540.1"
                     /translation="MSRSYQQYCPLARAMDAVGDRWTMLILRELFFAPKRFTDVETRL
                     DGIAPNLLSRRLKELEARGLIARRRLDPPANATVYEITERARGLQTAMLELSRWGIQF
                     LGPYDGEDKFSIEWLVPMLEEMADREAARGVWDVYQFVIGGESLWVEVADGDVTVRAG
                     EPPRAPDLRGEMDEETFMAIGFGTLDPVDALAQGRAVAEGDLTKTPTALAILSPAMIF
                     GKAATEAGAPAL"
     gene            20758..>22711
                     /locus_tag="VEV43_08205"
     CDS             20758..>22711
                     /locus_tag="VEV43_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYP23541.1"
                     /translation="MERLGRFVARHPRRILIVTVAFVALGAVIGPTVSAHLSSGGFDD
                     PSSESADAARRIEEIFGVSDPDVVLLVRAREGSVDDAAVAREGAALTRSLAQEEGVLR
                     AESYWSLGGAPPLRSIDGTKAMILANLEGTQNDKHDLLEELGPRYSGTSSAIDVSVSG
                     FSEIYRQVTHQVEEDLVRAELVAIPILLVLLLFVFRSPVAASLPLGVGLVAVVGTLVV
                     LRAIAAITEVSIFALNLTTGMGLGLGIDYSLFIVSRFREELGAGASVEDSVTTTVATA
                     GRTVLFSAFAVAASLSALLLFPIVFLRSFAYAGIPVVALAAAGAIVTLPALLAVLGEN
                     IDKWAVRRAPEPDRVGVWFRIAQWVMRRPVPIAAAGTLVLLVLGAPFLGVKLGLPDDR
                     VLPPGAPGRVVTDELRRDFAGNEAAATLVLAPGVDPVADREQVRRFAQRLSSIEGIAR
                     VDAATGSYVAGELLFFSERVGQRFAADDATYFSAVLAPTVEAQSLEAEEVVHGIRALD
                     APFAFEVAGPSADLVDGKESAFARIPIAVLWIALVTFVTLFLLFGSVLVPLKAVVLNL
                     LSLTATFGAMVWVFQDGNLSGVLDFTATGTIVLSNIILMFCIAFGLSMDYEVFLLSRV
                     KEEYDATGDNDLAVARGLDRTGRVVSA"
BASE COUNT         3235 a         7674 c         7878 g         3924 t
ORIGIN      
        1 ccgcgccgag cgcgagttcg tgatgcagcg atacatcgac aagaacatcc aggcccgccg
       61 gctcgcgccg ggtgcccgcg gcgcgatcaa cgacctcgcc gagcacggcg cgacgctcgt
      121 gatggacgac gcgccagcga aggccgccgg gaagctgctg ccgatgttcg gggtgccgtt
      181 cctgccggag ggtgtcggga cggcgtacct gaagcggatc gcgccgttcg tggcggcgaa
      241 gaacgcgtcg cccgcgcaag tggacgcgat gtaccggcag atggtggagc tggtgcactc
      301 ggagggaggc ttcgcgcagc acgcggtggt agtgacctcg gacagccgct acctagggga
      361 gattcccttc gcctcctggt aggggggccg ggctcgctct acgaaccgcc tcacggcctc
      421 gttcatgttg accggaacga ggcggatgtc ggggtggtca ctctgccaga ccctgaagac
      481 ctcgtcggcg aagccctggc cgacccactc cactccacgg aagtcgacga agacctcctt
      541 gaactggtcg aggttcctag tcagacgctt ggcctccgac cgcgacacga aggagacccc
      601 gtactcgaac aggcggacga ccgtacgtgt cgtgtcgaag tccagggtgt ccgggtcggt
      661 gtagtcgtcg aagacgtcgt tcaacgaacg gagggtgtgc ggatccacgg aaaaggacac
      721 atgcgtcccc ctgcgtgcac cgactcgcgc aatcgcctcg tcatgccgga cgttgtcgac
      781 cgtccatctc caaccattgg tcgtgagggt gaagaggtcg acggccttcg acgtaaagaa
      841 gatgccctgc cccgtatggc gggacgggtc cgtcgtcagc ttgcctttgg ctatctcctg
      901 aatggccgca aggtggtctt cgagtcgctt ggctctcctg acgttctcga acgcgccgat
      961 cccctcgtcg gagaccacaa agccctgcgc gtccccaggt aggacggaaa tgctgacgat
     1021 gtcggccccg gaatgttcga tcgcgttgtt cagcatctcg gtgaacgcga acctggcgat
     1081 gtcctgagcc cgttccggca ttcctccgac cgacgggact tcgaggagta cctcgcgcca
     1141 gactgcgtcc tccagaagac cggcggtctc gaactgcttc ctccaggcgg ccgccagtgc
     1201 gtagcgacgt ccgcggcccg ccccgatcgc cttcaactcg ccggcctcga ccatccgacg
     1261 gaggtggtag tgcgccgcct gtctcgtgac gcttgctgta cgggccacgt cgccgctcga
     1321 aatctcgggc gactcggaca gaaaggtccg gatgagcgag gggatggagc catcctcatc
     1381 gaacgtcggc atctttacat ttttaccttt ataggcctga tagtcaaaag gtaaagccgt
     1441 ggccgggggg gaaacgggtc cctcggagct cccaaaggta aaaaggtaaa aaggattcat
     1501 ccggggttgc cccacaaagg taaagcggcc gaccgggccc gcgtgcgccg agatccgatt
     1561 ctgtagcggt atgcggacgg gaacggatct cgacgcgaac gggccgcagc ctcggccccc
     1621 ggctcagcag tcggtcacta tctcctcgca gaggacctct acccggaccg cgaagttcag
     1681 gttctcgccc tggagcttga acgtgttcac ccctaggacc tcgccgaact tgttcagtag
     1741 cggccccccc gagttgcccg ggttgatcgc cgcgtcgatc tggacgtagt tcgggtacag
     1801 ctgcgagacg acgccggtcg tcgacgtgcc ggccaggccc gatgggctcc cgatcgccac
     1861 cacgaagtcc cccaccgacg gctcgtggtc gtgctcgatc gcccactcga gcgcggggat
     1921 gtcggcggcg acgtccacga ccgcgaggtc gttgcccttg tcccacgcgg tgacctcggc
     1981 cgggtagcgg gtgccgtcgt ggttcacgta gacctccggc ccgttcggtc gcagcgcgtc
     2041 cttgatcacg tggtggttcg tcgcgacgac ggcggtgtag ccgggatcgg gctggaggtt
     2101 caccgcgaag cccgagccga ggacgccgtt cgtgagctcg atcgtgacga tggactcgcg
     2161 cgcgcgggcg actatgtccg ggacgttcgg cgggaggccc tcggagagct cgttgacgcg
     2221 agagtacaag gggtcgaaac ggtcctcgag ctcgccgacc tgccgggcca cggccgcggc
     2281 ggcggacgcg tcggcgctct ggtcctcttc cagcaagacc tcgatgtcgt cgatctgggc
     2341 ttcgagctcg ccgatctgcc agacggcacc cgcgcccacc ccgccgacga cgagcagcag
     2401 cacgactacg gcggcggccc tcttccctcg cgtcacgaag ggaacgctag cgctcccgct
     2461 ggtagtagat cgcccagccc gggcccgcgc cgttctcgcc cgcccggtac gcgctcgcac
     2521 cgatgtggat gttccccttg gagtcgaggc gcacgttctc gaactcgggg atcggcgaca
     2581 cgccggtgaa cagcggctcg cgggtcgtcg tccccgagcg gacgttcttg gccgagaact
     2641 tcccggccgg gatgcgggcc caatagaagt accagtcgga ctccgtcgtc tcgttcgtgg
     2701 cggggtcggg gctcccgtac cagacgaccg ccgcgttgcc cgcgtcgtcg acggcgatcc
     2761 acggcgagaa cccggcgtcg ccgcgggtca gcggcacgga cttcgtccac gtcgcggcgt
     2821 cgtcggtcga ggtcgagtac cagatcggcc ggtccttgtc cgtcatcgac gagaacacga
     2881 cgtgcaactt gccggtgcgg tcgagcgcga ggctcgggta gaggtagctc atcgggttcg
     2941 gcatcgcggc gacgaggttc gacgtccacg tcgcgccggc gtcgtggctc gcggcgacga
     3001 acagcttctc ggggccctgg tcgtcgaggc tcccgagcgc ggcccccgcg cgccgcgtgt
     3061 acggaaggta gaggtcgttg ccgtcctcgg agacgacgag gttcccctgc cacgtgaacg
     3121 caccctcggc ccccgcgccg gcgaccatcg acgtcggctg gaacgtcatg ccgtagtcgg
     3181 tggacttcgt gtaccagatc gccgacggca tgcagcactg gaggtcctgg tagctgacgt
     3241 ggatcgtgcc ctccggcccg ccgacgagcc agggccggtc gacgatgaga ccgcttgccg
     3301 cgaccggcgt cccctcccac gtcccgccgt tgtcgcggct gtgtccgacc gagaggttcc
     3361 cgagccacgt gtcggcggag tacatggtcc cgccgtcgtc gaacgcgacg tcgcagtcgc
     3421 cgccctcgaa cgtgtacgtg cggccctcgc ccgcgtcgtc gttctcgacg cgcagcggct
     3481 cccacgtccg gcccccgttg cggctgcggt ggaagtacga ctggttgttc tgggtgttcg
     3541 gcacgcccga gggcgcgcag gcgagcatga ccttctcgtt caccgggttg atcgcgaggc
     3601 tcgtctcgcg gtgcgcctgg tcggcgtgca cgagcacggg cgcgccccag ccgggcgcgg
     3661 gcttcggcgg acgcggcccc ttcggagcgc cgagagacga cccgacgagg gcggccgata
     3721 cggccacggc cgcggctatg ccaaagacct tcttacgcgc acgcacagag ggtgcctcca
     3781 gtcgctcttg ccagttcagc cggtagagga ttcggcggcg aagcccgatc tcccgcctgc
     3841 ggagcggcag tggtcacggc tgcgacgtac ggggagcggc tagcggccct cacctacgcc
     3901 cagaacgtcg aggttcgccc cgatctcgac cagcgcgttc tggatctggt cgcccgtgca
     3961 gcggccggtg ctcagcgact cctcgtagtt cgtcatctcg agcgacggct gcgctatgcc
     4021 cgccgcgttc gcgccgcgca cgtcgtactg ctcggccggg atgatgtagc cgatgtggtc
     4081 gttcgccatc ccgagcacca tcacgttgcg ggccctcgag aggtagtcct tcgtcgtgag
     4141 gctgatcgag gcgaagatct cgcccggggc ctgcatcagc acgaccgcgt cgccgcggtc
     4201 gccggggacg aagccgcggt cgtcctggct cggtcccggc gtgccgatgc ggaacccgcc
     4261 gaccggcgac ttgacgctga gcggccctgc accggcgcag ctcggcgtgc gtgaacgccc
     4321 tttctgctgg acgctctggg agggccctgc ggcgtggggg ccggggatcg tcgtgtcgcg
     4381 catgaagatg ccggccttgt tcagagctac gagcgaggcg ttgtcggcag ggatcgtgag
     4441 gatgcgctgc tcggcggcga tgtcgttcga gcgcagcgtg tacccgtagc gcgcggactc
     4501 gaagacgagc tcggcgaggc cgtagccgat cccgcggcgc ccgaagctcc ggtgcacggt
     4561 ctggtccccg aggccgcccg gcacgaatac cgtgctgccg ccccagagct gcccgaggcg
     4621 ctccgcgagg tgaccgacga acccgctgtg catgtcctcg ttgccggcgg tgacggtcgg
     4681 gtgcgccgag aagctgacga gcgtcgcgat cgtccgcgcg gccttgccct tcgtggccgg
     4741 gtcggtcgag ggcagcgcga ccgccctcag ccacgtcagc tccgcgtcgg gaacggcgcg
     4801 ggtgacgatc cggcggtcga cgttgaaggc cttcgcaggg atcgcacccg tctgcagcgt
     4861 cgcgggctcc atcttcgaga ccgcgccgcg gacggagtcc ttgatgcggt cacggacgaa
     4921 ctcgtagtac caggacggtc cccgcgcggt cgtgtcggcc gaagcatggg tgtgggtcga
     4981 cgcgatgacg aagttctcga ccgggatccc gagctcgtcc gagaggtcga cgacgatcgc
     5041 ccgcgtcccg caggtgtcct gcggctgggc cgcgtcggcg tcgtcggggc agatcgacgg
     5101 gtcgtacccg tagaaccacg agacggtgtc gagcacgacg aacaccagcg tgtcctcgcc
     5161 gttgccgatc gccatcgcgc gggcgtagat gccgaggtcg gaagccgcgt cgacgcccgt
     5221 cgcccaggca cggtcgaagg tccgcagaca cgaggggtcg gaattcggca ggtactcgta
     5281 gcccatggga ccgcagtcgt agccgccgcc gtgcgggttc cacggccccc cttcggactg
     5341 aggaacgggc gcgatcgact cgttgtgcgc gccgaccagg aaggtcccgc ccgcgagcct
     5401 cccgccggcc gggctcgcag ccgggctcgg cgcgggggcc ggcgtcgcct gcggcgaggc
     5461 cgaggtgctc ggggacggct ggacgagaga aggcgtcacg tcgggggtct cgacggagac
     5521 gaggcccgtt gtcgcccacg cggccccgag cgcgagcgcg cccgtgagga cgccgctcaa
     5581 caagccggtc aggtatcggc gcatggccgc tgagttcgcc ggggggcccc acgctcctgc
     5641 cgcgccctga cgaccgctcg tcggcgttcg cgccgcaagc gacggaggaa tggacacgtc
     5701 gcgaggcgaa ccaaccgccc atgacctcca aacgcgtttc gacgaaggtg atgctcgcgg
     5761 ccctcatggt cgcgggatcg ctgctcccgt ccatccctgc cggcgcccag gacggcagcg
     5821 ctgccaagtg cgtcgacggc atggccggca tctacccctg caaggccatg gacctcctgt
     5881 cgcaccggac gttctcggcg gccgagggcg gcggtgtctc cgacgtgtgg ggctgggccg
     5941 acccggagac cggcaacgag tacgcgatcc tcgccggtgg cggcggcgtg cgcttcctcg
     6001 acgtcaccga ccccgcggcg cctgtgtacc tcggcctcat gcccacgaag ggtgacggtc
     6061 agacgctgtg gcaggagatc gagatcctca acgaccacgc gtacgtcgtg tgcgacgcga
     6121 tcccctgcgg gctccagatc ttcgacctca agcggttgac cggtgtcgag gccgcggtcc
     6181 cggtgtggcg gcccgacgtg gtcctgccga tcggcacctt ccactcgatc gcgtcgaacc
     6241 ccgagacgaa ccacatcttc ctgaacggca taggcgccgt cgtcggatcg ccgacgggga
     6301 ctccgctgat cttcgacgtg agccagccgc tcgcaccggt gccggtcggc ggcatgctcg
     6361 acgacggcta cacgcacgac tcgctctgcc cgatctacaa gggcccggac aagcgcttca
     6421 agggcaacga gatctgcttc aacttcaacg aggacacgat cacgacctac gacgtgaccg
     6481 cgaacccgca gcagcccgtg cagctctcca aggtcacgta cgacaatgcg gactacgtgc
     6541 actcgggagc gctgacgaag gaccacgcgt acctgatctc gaccgacgag ggcgacgagg
     6601 tcgagtatct cattccctcg acgctctaca tctgggacgt ccgcaagcta gacaagccga
     6661 agctgatcgg gacgttcgtc gggaagacgc tgtcgatcga ccacaacgtg tactcggagc
     6721 aggacgcgct ctaccacgcc aactacaaca acggcttccg gatcctcgac atggcgaacg
     6781 cgagcaaggg caagctcaag gaggtcgcct ggatcgacac gatgccggcg gccgactccg
     6841 ccgactacga cggtgcctgg gcggcgtacc cgtacctgcc gtcgggcaac atcctcgtcg
     6901 gcgacatgaa cggcggcctg ttcgtggtgc ggccgcacgc caagatctac cgctcgctcg
     6961 gcgtaaagcg ctgacgagaa aggaggcgag gccgcccgcg cggccccgcc tccccgttcg
     7021 agtcgctcct atcgcgtcac acggcgcagg cgatcctgcc cgacgtcacc gcggtggccg
     7081 tatcgaagct ctcctggtcg gcctggttcg tcgcggtggc gacggtgcgc gtccggtaga
     7141 agaagcggcc gggctggcat gcgaccgtga tccgcttgtg ggcccggtcc gtgtcggtga
     7201 ccgccttcgg cttgcagccc aagagggccc attccccggc ctgccggaag tcgatgcaca
     7261 ccttgagtgc tatctccttc tggatcgtcg ggcagaccat gccgcccctc gccttgatct
     7321 tcgccccctg cttgaacggg acctgggccg tggcgccgtt gtcgcagttg ccgttggtca
     7381 gcgtcgacag cacgtagaac aggtacacga tgtgcggcca gtcgttgggc agctcacctg
     7441 cgttgcccat cacgatctgg tcgacgatcg cggggtcgat caggaagatc gggcccgggt
     7501 cgggtacgaa gaccgtgtcg agcacgtcct cttcttccgg ggtctccacc gcgacctgcg
     7561 cggtggcggc cgtagtcatc aatgccagtg ccgtcagcga gcagagcacg cctcgggccg
     7621 cgcgcgaacg aatgaatcga gcgttcatct aggttgtccc ctcccctgag gaattcccac
     7681 cgttctccca ggcgtccgcg gtgctgggag aagttcgtca gccggcgccg tcaacgtacg
     7741 caaaagagga agttcgggga atagacaaca aggacgattt ccgaggcgct acggcggact
     7801 agtcaaggag cgggttgagc tcgatgcccc gtcacaccgg gcacatgttg cacatctgca
     7861 tcgcagcgag catcgccgcg ctcgctcccg cgcacgcgca accactcccc ggcgaggagc
     7921 cggcgtgctt cgacaaggaa gcgacgctcg tcgggaccga agacgcggat cgcttgaagg
     7981 ggaccgagcg cgccgacgtc atcgccgggc tcggaggcga cgacacgatc cgcgggctcg
     8041 gtgggaacga cgcgctctgc ggagggccgg gcctcgacgt cgtcaacggg gccggggggg
     8101 cggacctgat cgccggagac gagggcagcg accttctcat cggcggtgac ggcgacgacc
     8161 ggctacgggg cggcgccggc atcgacttcg ccgaaccgaa cgcgggcgac gacttcgtcg
     8221 aaggggggcc gggcttcgac gggatcagcc tcggctccgc gcggcgcggc gtctacgtga
     8281 acctccgcaa ggggatcgcg cgcggcgacg gccgcgatcg cgtcgccggc gtgagcgaca
     8341 tcgaggggtc gcgcttcgac gaccggctgg tcggccctta cggcgaggga cagatcctgg
     8401 cgctcgacgg ggacgaccgc ctcgtcggag gcagccacga cgggatcttc atccccggcc
     8461 cgggcgacga cgtcgtgatc ggcggacgtg gcgggggcga cctgatcgcg ttcccgttcg
     8521 ccgaggcggc ggtggtcgtc gacctgagag ccgggagggc gcgcggtgag ggacgcgact
     8581 cgatctcgcg cctcgacgac gtcgtcggga ccgaccgcgc cgaccggatc ttcggcgacc
     8641 ccgggaggaa ccggctgttc ggcgccccgg gattcgacgt gctgcgcggc aggggcggcc
     8701 gcgacgaggc cggatatctg ttcgacaaca cgcgcgtgag cgcggacctc gagcgcgggc
     8761 gggccgtctc gtccgggacg gacgagctct actcgatcga ggacctgagc ggctcgctgc
     8821 gtcccgacac cctgagcggc gacgaccgtc gcaaccggct cgtggggctg gcgggtgcag
     8881 acgtcctcga ggggagggac ggacgcgacg agctcgacgg tggggacgga ccggaccagg
     8941 ccttcggggc ggggggcgcc gaccgggtgc gcggaggcgc gggcgaggac gtggtcggcg
     9001 cggggccggg tgacgacatg ctgttcggcg gcgagggcgc ggacgagctc gacgggaacg
     9061 cggggagcga caccgcggac ggggggccgg acctggacag ctgcgtcaac gtcgaagagc
     9121 agcagaactg cgaggtcccg atctacgcgc gctcggggcc gctcgcgctc ggcctccggc
     9181 cggggatccc ggcatggatc gggtcgcggc cactgaggct gctgtggtgg ttacggctga
     9241 cccgctgacg aaaggccctc ggggcgtctc ggtttcgatc agtcgctgcg ccctaccgcg
     9301 ttccgcacga ggatcacgaa tccgacgatc gcgaacacga tgccggcgat cagctggaac
     9361 gcggagccac cgtcgccgaa gacctccgcg tactcgagca cgcccgccac gccgatgacg
     9421 agcagcccgg ctccgacctc tcgcttcgca tatcgcatca tcctggtatc agcctccgcc
     9481 cgccgggccg tcgtcccgcg tccgcaagac gtggaaagcg tacccaagca tcgtcaccgt
     9541 cagactcccg aacgctagac ccatcgcgac gagacgcgga gcatctgccg cccgccggac
     9601 gaagaagaga gctgcgatcc cgaacgtgac ggcgaggccg gtgttgcgcg tccggttctg
     9661 tcgttgcacc gccggatcgg gtgactcgcc cttcgtcagg aaccggtaga cgaaagcgcc
     9721 ggctatcccg gcggccgcgg cggccgaacc caggaacagc gtccatccgg tcgagcgttc
     9781 ctgcgccggc atcgccccga cgtttcccgc cacgaccggg agcaccgcga gggggagagc
     9841 caggaggacc caatcacccc aggtccaacg cctcctgact ctcccctcgc cggtcatcgt
     9901 ctcgttacct cgttctccgt caatcccaga aggtcaacgt gtcacccacg tccgacgcta
     9961 cgtctgcggc gtcgccgagg aactcctggc ccgcgtcgac tgcgtcgccg acggcgtcgg
    10021 tcgtggcgtt ccaggcgtca ctgaccatct cctggccgcc ctcctcgtac caccactccc
    10081 cgagctcctt gccgaggtac tcgccggcct cgctcgccag ccacgcaccc cccacgcacg
    10141 cgactgcccc gattcccgca gtcgcgactc ccgcggccgc acacgccgcc gtgacggctg
    10201 cgctcccggc aaggcttccg ccgaactcca gcccggtgcg cgtggcggcc tcaccccagt
    10261 cgccgccgga gttgtcgtag taacccttga acttcccacc gaactcgagg acgtctcctg
    10321 ccgtgccggc ccagcccagg tacttgctgg ccttcccggc caggtcgctg tgcctgtgca
    10381 tcccgtcgga gatggcgtcg gtcgtgttct cgaacccgtc cgcgtagcgc tccggcacgt
    10441 agttgtccag ccagtcgtcg gcgtcgtcac cgaagcccct gatctggtcg gcgagaggac
    10501 cccagttccg ggggttcgtc gcctcttccg cgacttcacc gacaccctga aggacgtccg
    10561 tcttgctcaa cctgaaccct tcgtcgccgt cggaagagcc gtcgccgctg tcgccggtcg
    10621 ggttcgggtc cgaagcggcc ccgcctccgc cgtccgcgat caccatgtgg ccgtcgaact
    10681 cgacgaacga gatcgggttg ccaccggcga agctgtagcg gttctgcgtg agcgggtcga
    10741 tgctcaggct caggtcgctc aaagacccgg cgaagacgtc gaactgcagg aagcggccga
    10801 cgtcggggcc gaaacgccgt gcccccatgt cgatcgaccc ggaccccgag tcgaagcggt
    10861 ccgcagtgaa gcggaacggg ttgagcggcg ccttggcgtt ctcgtcgttg gcgctgaccg
    10921 cggcgtcgct cttgccgtag gcggtgtagc cgtaggaggc gcgcgctccg ccggcctcct
    10981 tgagcagcat cgagaccgac ccgtgcacgt cgtaggcgta gttgtagttg tcggtcgtcg
    11041 tccccttctg gtgctgcatc gagacgcggt tgccgtaggc gtcgtaggag tacgacttcg
    11101 tctcggccgt ggtgccggtg atctgttcct gagtcacgag gccggtgagg ccctggtagc
    11161 tgaaggccgt cgtgcggtcg tgcgtcgtcg caccgctgcc gtggtcctcg acctccttga
    11221 tcacgcggtc caggccgtcg tagctgtagt cggtgttcgt gttgtcgtcc gaggcgaacg
    11281 accggtagtt cgtcatgcgg ttcaggtcgt cgtaggcgta gtcgctcaac acccccgtcg
    11341 ccggcgagcc ctgcgcggac ttcgagcaat ccgtgtcgac acccgtcgcg cccgtcacgc
    11401 agtccaggtt cccgtagtcg tcgtagaagt acttgttgac gttcgacccg gcctggacct
    11461 gcgtgagctg gttcgccgtg tacgagtagt cgatcgtcgc cgtccccttc gcctcacggg
    11521 tgacgttgcc ggcggggtcg agcgtgtagc tcgtgacgcc gctgtggccg tcgtcctcct
    11581 tcacgaggcg atcgcgcggg tcgtagacga acttcctcgt gcacgggctg gcagggcggc
    11641 acttcgccgt cgtgtccggc ccgtccatcg agtacacgtc cttcgtgcgg tggccgttga
    11701 cgtagaagcc ctggtcgctc aggtacgaca ggtcgtgcga ctcgacgacc ttcatcgtcg
    11761 cggtggcgtc gtcacgctgc ttcgtgatga gcgtgtccag cttcccgttc tggaagtacg
    11821 tccagttggt ctgctgcttc agctgccacc cgttgacggg gtccgtggtg caggtgccga
    11881 gggccttgtg gttcgccctc tgctgctccc acccggtgac cgtgaagtcg ttgtcgatct
    11941 tgcggtcgtc cgtgcagtcg ttcggggtct tgcccttgtc gatctggacg tccagccagt
    12001 tcgctgcgtc gtacgtgaac gtgctggtct tcccgccggt caaggtcgtc gacccgggca
    12061 ccgccgttgc gtcgtcgacc cggcggatga ggttgccgtt gaggtcgtag gcgtattccg
    12121 tagcggtgta gttcgtggtc gtgtcgctga cgtcgaccgc cttgcggtgc cggatctcga
    12181 tgcggcggtc gagcgagtcg tacgtcgcct tcatctcgac gcgcttctcg ttgccgctga
    12241 tcccgctgtc gtcgacgatc gacttgacgt tgttgtcgag gtcgtactcg tacgacacgc
    12301 cgccgccgtc ctggtcggtc ctcgtcttga gggtgccgtc cgggtagtag gtccaggaca
    12361 tcgcccgctc catgttcttc gtgtccgggc tgtccttcat ctccgggatc ctcgacttct
    12421 gccagccggg ggcctcgtag tcgaagtgga cgatcgggtt cggggccgtc ttcgacgtct
    12481 tgacccaccc cgggtcccag tgcgtcacga ccgtccagct ctcgtgcgcg tctgccggag
    12541 gcgcgaggct cgtcacgggg agcgacgtgc gcacgagccg cccgttcttg tcgtacttct
    12601 tgtggacgta ctgctgctgg aagccggtct cctccggcag cacgttgctg aacgggagct
    12661 cgaccctctc cacccggttg acgtcgtcgt acacgatcgt cgtcacgtag cgggagaagt
    12721 tctgcttgtt cggctcggcg tccggcgacg cgtcccaggc ccgcgggctg atgaccttca
    12781 ccttgttccc gacggcgtcg tagcggtaag cggtcttcac tttgcggccg acgccttcct
    12841 cgtagggctg gatcacccgg gtgaggaggt tgcgggcgtc gtagtcacgc gtggagacgt
    12901 ggttcgactc gttcgtcgtg ctgatcacgt tgctgttcgc gtcgtacgcc atcttgcgca
    12961 cgtggttcag cgggtccgtg accgtgagac gccggtgagc gtcgtcgtac gtgtacttcg
    13021 tcgtgaacgg ctcggccgat cccgtcgcgc cgcacgtgat cgtcgccttg ttcgcccggg
    13081 gcgacgtgac cgagacgagg ttgcccgcgt tgtcgtagca ggcgtgggta tggagcgact
    13141 cgatgacggt cccgtcgggc ctgacgttgt gctgcgtctg cgtgacgacc cggtccagcg
    13201 cgtcgtaggc gtagatcgtc gcgaagtcgt tggcgacggt cgccgtcgcg gtgccgttcg
    13261 gcctgatcac cttggtgacg cggttcgccg cgtcgtactc ccactccgtg cgatcggact
    13321 ccggcccggt gcgcgagagc ctccggtcca tggcgttgta cgtcatggtc gtgggcgccc
    13381 ccaaccccgg gttgcctgca ccgaaggccg gcgtgaactg cgtcacgagg ttgtcgttcg
    13441 ggtcgaactt cgccgcgctc cagacgaggt tgccgcggtc gtgttgcgtc gacttcggcg
    13501 agctctgctg gccgagccgg tggaagctgt cgtagtagaa gtacgtccga tagtcgcgcg
    13561 cctgcgcgtc cgtgtgaccc gtcacctcgt actgcaggtg ctcggggtcc tggacccact
    13621 cgagcagccc gtcgtcggtg tagccgaact tcgtggtgtt ccccttggcg tcgacgatct
    13681 tcgtcgcgag cccgttggcg tcgtacgtcg ggaacgacgt caccttgctg cgggcgtccg
    13741 tgatcgacga gatcgtgccg gggtcgaccg tgcccgtgtt ccacgcgtag gtcgtggagt
    13801 agcggctgcc gtcgggcagg cccgcgggct ccggctcgat gaccttcgtg atgttgccgt
    13861 tggcgtcgga ctggaagacc caggcgttgc cgcgcccgtc gatccgtcgc tcgagctggc
    13921 tgacgtgcgg gatcgtccga cccgccttcc agtacgcgag cgcgtcgcgc gtgtcggccg
    13981 cgccaccctc cgccttcacg gcgaggttgt tgtagacgag ctgcgtcgcc gtgatctgga
    14041 cgttgttgcg gtccatcacc ttcacgtcgg tgagatagcc gttcgcgttg tacgtgtact
    14101 gcctgttcgc cccgttcggc tccgtcacct tgctgacgtg gaagtccggc gtccacgcca
    14161 cgttcgtgac ctcgttcttc gggttcgtca gctggacgac gcgcccgttc gagtcgtagt
    14221 cgtacttcga aaccctcgac agaggcgccg tgaccgtcgt cagagtcagc gtgttgtcgt
    14281 aggcgaacga cgtggtgttc ccggtgcggt tcgtgcggga cttgagcttc caccggtcca
    14341 gcgagctccc cgggccgtaa taggtgaacg tcgtctcggc gccgcgcgcg tcgcggacgc
    14401 tgaacaaccg gttggactgg ttcggcgtct tcgaggccgg gttcacccgg tccgccgcaa
    14461 gcgggatcgc cgggcccgag ccgtccgagg tcgtgtacgt gaacacgaac gtcctgctcg
    14521 gcagcgcggt cccgtctgcg ttcgtgccgc ctcgctgcgt caggcgcagc aggttgccgt
    14581 cgtcgtagta ctcgaagtcg agcgcgctcc cgtcgtggtc ggtgatcgtc tggatcttgc
    14641 ccctgacgtg gggcttgtgc gcctcggcct tcgagtagta gtcgatgttg aagctgcggt
    14701 tcggcgcggc gttcagggca ccggccgcgt ccgtgacctt cgtgatccgc ttcttgaagc
    14761 cacccgggtc ctcgcccgga ggcaccgtct ccagcgtgaa cacgatcttg ttgccgttgc
    14821 ggtcctcgac ggacgtcggg taaccctcgg agtcgtagaa gaacgtcgtc cggtcgggct
    14881 tcgtgagcgc ccacttcttc gtgaggtccg tcgtcgagta ctgacgcagg aacaggtgga
    14941 cgcccggcgg ctcgtcccac ccgccaccgg cgttctgcgt gaaccggtgc gtcgtgccgt
    15001 cgccgtcggt gaactcgatc cacttgttcg agcggccggc gatggtgtcg gcgttgttgg
    15061 ggtggatgtc gagcggcagg ccgaacctgc tgaggctcga gatcgagagc gagaagttgt
    15121 tcccgatcgg cgagtcggac ttgtcctcga ggctgttgta cgtgatgtcc atcacggtcg
    15181 ccagaccacg gcctggcgcc atgaacggcg tccaccgaag gatcgagttg ccgttggcga
    15241 cgttcacgag gttgcccatg ccggcgccga ccgactcgcc cgtgtagtgg tagtagcgct
    15301 cgaggcccag cgccgtcgcc ttcagtgcct tgttgatgag gacggggtgc tcgagcggct
    15361 tgttgccctt cgccgtgaag taggccgagg tcgacttttc ctgcaggtcg aagcggagct
    15421 ggtactgcgc ccggtcgatg ccctccgcca ggggcggcgg ctggacctgg acgcggagcg
    15481 tccgtgtgcc cgcggccgcc aggttcgtgc cgaacgccac ggcggtgccg tcgacgaacg
    15541 tcgaaggcgt ggcagggttg taccagcggt accgcagctg gaggtcggtc ccggtccacg
    15601 acaccgtgct gttgttcttg accgtgacgt cgaccgggta cgaggtctgc gtgactgctg
    15661 ccgggtcgta cgacatcgcc tgcgggacgg cggtcgacgt gaacccggcc ttgtacttgg
    15721 tcgtaccggc gttggagacg gtcacgttgc gcccggccga cgtcgtcgtc tggccgtgcg
    15781 agtcggtgac gcgcgaggtg agcgtgtgga ccccgtcata cgcggggttc gcagggtcga
    15841 gcgtgttcca ggcgacgctg tacggcgacg cggtgtcctc tgcgaacttg aggccgtcgg
    15901 agaagaactc gaccttcgag acgccgaggt cgtcggcggc gttcgccgag acggtgacgg
    15961 cggtcccgct gacggtcgcg ttcagggcgg gggccgagat cgaggtcgtg ggcgccgccg
    16021 agttgccgac gacgaccggg acggccgcgg acgtcgtgac gttgccggcg tcgtcggtgg
    16081 cgcggaccgt caggttcctg gtgccgttca cgaccgtcgt ggagttccag gtcgcctccc
    16141 acggtgccgc ggtgtccgtc gacacgagag cgccgtcgac gaggaactcg accttgtcca
    16201 cacgccggtc gtccgaggcg ttcgcgacga tcttgaccgc gctgccggac acctgcgtgt
    16261 ccttcaaggg cgaagcgacc gagatcgcag gccccttggc tttgttgccg tcggtgtaga
    16321 ccatgacgag cttcggccgg agcgtcggcg agaccgactg gtcgtccgcg tagtacgtga
    16381 agcgacggcc gtcgacgagc gtctcgtcgg agcgtttgag gatgatgccg tagttgggca
    16441 ccgaccccga gacccacttc tgggccacgc cggcgaggtt gaagacgtcg gtctccgcgg
    16501 gctcgtccca gtcacgctcg gagtcgaccg gcgtgccgtc gatgtcgccg ccctgggccc
    16561 cccacttcac gccgccctgc gtctcgtacc aggtcgcgcc gtcgccggtg caggtggaga
    16621 cgccgctgcc ttccttccac ggagaggtca cgcggtgggc gttgattgtg tggtcgcccg
    16681 tgaccatcgt cgggtgccac agcgacaggt cggcgctgtt gacggtcgcg tgagcgggca
    16741 cgtcgcgcat gtcgaacatg agcgcgctcc tccacaccga cgtcgaggtc gtcccgaagt
    16801 acagcgacgg ctccttgccg tagttcgcgc agctcgcgta cgacgagtac ttcgccccgg
    16861 aggtcgcgac gccgtccgtc gggacgggct ggaggatctt cttcgactgc ccgtcggccg
    16921 gcagcgtgac cgtcacgccg ttggaggtcg cgcctccggc gaccaccttg tagctgaagg
    16981 acttgctggg ggccgcggtc gcgtcgacga acgaggtcgt ggcgacgtcg ccgatgctcg
    17041 tcagcagcgt gctcgccgac ggcgcgaagg tcgcggtcgt cccgcggtgc acctcgtagc
    17101 tcgtgaagcc cgtgccgttg aacttcgtcc actcgagctc ggccccgttc gagtgcagcg
    17161 tctcgggctc gagcagggtc accgcggcgc tcgcgtaggt gacctcgagc ttgggccgga
    17221 tcgtcgtctc gaagtactgg cggctcggca tgtcggggcc gctcgcgttg gccggctcgg
    17281 tggcgcgctt cagcatgatc ccgtagttcg gcgacgtacc ggccagccag tccttgaccg
    17341 tctgcgtgac gccccagttc atccactgcg ccgcggtgct ggcggggacc gtgaagctcg
    17401 acagcgtggt cgggtcgaac gtcaggcccg acgtcgtggt cgtcagcgac cagttcgacg
    17461 tgatcttgtg cgcgttgatc aggtgggacg tcgacccgca ggtgtaggac ccggtgtaga
    17521 cgcactcgcc cgtgtagtag ctcttgaact gggcgtcggt gacgttcgcg ccgacgggga
    17581 tcgagctcag gtcgaacttg taggcgccgc gccaggtgtc cgagtcggtg ccgacccaga
    17641 tcgtctcgta gtcccagggc gtgcaaccgg aacagttcgc gatgaagttc gcgtcgagcg
    17701 acggcggctg gatcgtgatc gtcgggtcga ggtagaccgg gaactgccgc tcctggtcgt
    17761 gaagccactg cgcgtcgacc gagaggtcga ccgcgtagcg tccgccgacc ttcctgacct
    17821 cgagcgaggc gtgaccgtca cggctgcgcg aggacggccc ggagtcgtac gcgtaagggg
    17881 gcgcgagcac gaacgcaggc tcgggcttcg gtgcgacgta gaacgcccag cccccgttgt
    17941 ccagctcctc gacctcgacg gggacgtctc tcagcgcgtc gaggtagaag cggtagcgcg
    18001 cgggtgccga agcgtcgtcg aggatcagcg tctccttgac gccgtcgggc agcagccggt
    18061 actcgacgtc gacgccgggg aagacgtcgt cgtagatcag gctcgacttg ccgggaaggt
    18121 cgtcgccggg ctcgggctcg ggcgtgggtt ccggcgtggc ggtctccgtg ggggaggcct
    18181 cgggcgacgg tgcggcggaa ggctccggcg acgggccggc cggctcctcg gcggacgtcg
    18241 ccgccacgtc accttcggcg cccttctcct tgggcgcccc gggctcggac ggcttctcgc
    18301 cccggggctc ttcggccggg gcctcggtgg caggcggttc cgtggcgggg gcctcggtcg
    18361 ccggctccgg ggtcgccggg gcaggcgtca cttccgggga tgctccgccg cccctctcct
    18421 tctcgcggtg ctcgcgcgcg gacatcgcgg tcgccgaggg cgacgccccg tcggcggtga
    18481 tctcgaagac ctgctcgccg atcgcgaagc gcatgaaccc gtcggtcgta tgacggcgga
    18541 acgacgcgcg gtacgcgttc gcgccgttct gccacgcgta cccctcgtgc gtcgcgtcct
    18601 cgagcgcggt gtcgatctcg cgccagttgc cgcgcgcgtc caggaagtgc acggcgccgg
    18661 agaacatctg gaccgttttc gtgccgtcgt cgttgcgata cgtcttcgac gtggccgtgc
    18721 gcaggcgctc gatctcctcg cgcccgccgt ctgcgttctg ctcccgagcg gggcccgggt
    18781 cgggttgcgc ccccgccggg aggaaaggca attgcgctaa cagcgcagtc accatgaaga
    18841 tgccaataga tcgaacggta atccagcgtt tgttcacgcc cgcctcccag ctcgcggcgc
    18901 ggccctcact ccgcgcctcc ccctcaacag cgggcttcat cgctcgtttt aaggaactgt
    18961 aaaaacccgt gttgcgacgc taagaccgca gcgcctcggg tccaatagcc cgccctagcg
    19021 gtttcgcgac ccgcgcggga ctaggcattt cccgcgaaaa acggtccctg ccgtagcgcg
    19081 cgtcccgggc ctgccagttg aacgtcacct ttaacggagt ggttaccgcg ggaaggaaaa
    19141 cgagcacggg gacagaacca gcgactcaag cgcgcgcgac cgcagttgcc cgttcgaagg
    19201 agaagccaat atgaaactca atagaaagca gttcatcgcg gtcgcgctcg ccggtgcgtg
    19261 catcgcgtcg acggggtcgt acgccgcgtc cgcgcaggac gcaaccctgg accccgccgt
    19321 agacccggag gtcgtcgcgg agtcgctctc gggcgagctc gccacgcctc ctcccgttca
    19381 gatcgcggtc gacccggccc ccgtggtcat cgaagagccc gccgtctcct ctcccgagga
    19441 ggagaacctc acgaacgtga ccgtccggga tccggggccg gtcatgcccg ccgaccccgg
    19501 tgcggtcgac tcggcggtcg tcgggtccgt gggcgcggcg cccgacgcaa cgtcgctggc
    19561 ggccgtcagc accaacgtgg cggacaacgc gggcagcggc gagtgctcgg ctacggcgtc
    19621 cgtcccctac aagcaggaca tcaagatctg ggggaacggc agcgtctcgt gcggatccgt
    19681 ccagcgcagc ctgaacgtga tcgtctgcct cgaagtccgt caggcaggtg cgtgggacga
    19741 gctcggctgc aagccgaaga ccgtctcgca ggccaagtcg acgtcgaaga aggtgtcggt
    19801 ggcctgcgtc cccggccgct tcctctaccg cacggtggtg tggggagcag ccacgtcgag
    19861 gagcggcaac cgctcgagca tcccgcagga caccgccgtg accagcggcc gcatcccctg
    19921 cgcgagctga tcgcccggta acacgcctct agagctcgcg ccggtgccgg agcgctacgg
    19981 caccggcgca gctcgagggg cgttcagagg gccggcgctc cggcctccgt cgcggccttt
    20041 ccgaagatca tcgcgggcga caggatcgcc agcgccgtcg gagtcttcgt gagatcgccc
    20101 tcggccacgg cgcgcccctg tgccagcgcg tcgaccgggt cgagcgtccc gaacccgatc
    20161 gccatgaacg tctcctcgtc catctcgccg cgcaggtcgg gggcccgcgg cggctcgccc
    20221 gcccggacgg tcacgtcgcc gtcggcgacc tcgacccaca ggctctcgcc gccgatcacg
    20281 aactggtaga cgtcccatac gccgcgcgcg gcctcgcggt cggccatctc ctcgagcatc
    20341 ggcacgagcc actcgatcga gaacttgtcc tcgccgtcgt agggaccgag gaactggatg
    20401 ccccacctcg acagctcgag catcgcggtc tgcagccccc gggcgcgctc cgttatctcg
    20461 tacacggtcg cgttcgcagg cgggtcgaga cgccggcgcg cgatcagccc gcgggcctcg
    20521 agctctttca ggcggcggct gaggaggttc ggcgcgatgc cgtcgagacg ggtctcgacg
    20581 tcggtgaacc gcttcggcgc gaagaacagc tcgcgcagga tcagcatcgt ccagcggtcg
    20641 cctacggcgt ccatggcgcg cgcgagcggg cagtactgct ggtagctgcg ggacatgcga
    20701 ccttggtacc aggctcactt gactttttca aggacgagct atacgcaata tagccaaatg
    20761 gaacgcttgg gtcgcttcgt cgcccggcac ccccgccgga tcctgatcgt cacggttgca
    20821 ttcgtcgcgc tcggcgcggt catcggcccg acggtctcgg cacacctctc gagcgggggc
    20881 ttcgacgacc cgtcgagcga gtccgccgac gccgcccgcc gcatcgagga gatcttcggc
    20941 gtctcggacc ccgacgtcgt gctgctcgtg agggcgcgcg agggatccgt cgacgacgcc
    21001 gcggtcgcgc gcgagggggc cgcgctgacg cgctcgctcg cgcaggagga aggcgtgctg
    21061 cgcgccgaga gctactggag cctcggcggc gcgcctccgc tcaggagcat cgacgggacg
    21121 aaggcgatga tcctcgccaa cctcgaagga acgcagaacg acaagcacga cctgctcgag
    21181 gagctcggcc cccgctactc cggcacgagc agcgcgatcg acgtctcggt cagcggcttc
    21241 tccgagatct accgccaggt gacccaccag gtcgaagagg acctcgtgcg cgccgagctc
    21301 gtcgccatcc cgatcctcct cgtgctgttg ctgttcgtct tccggagccc ggtcgcggcg
    21361 tcgctcccct tgggcgtcgg cctcgtcgcg gtcgtcggga cgctcgtcgt gctacgcgcg
    21421 atcgccgcga tcacggaggt ctcgatcttc gccctgaacc tgacgacggg gatggggctc
    21481 ggcctcggca tcgactacag cctgttcatc gtgtcgcggt tccgcgagga gctgggggcc
    21541 ggcgcgagcg tcgaggactc cgtgacgacg acggtcgcga ccgcggggcg cacggtcttg
    21601 ttcagcgcgt tcgccgtggc cgcgtctctc tccgcgctgc tgctgttccc gatcgtgttc
    21661 ctgcgctcgt tcgcgtacgc gggcatcccc gtcgtcgcgc tcgccgcagc cggcgcgatc
    21721 gtgacgctgc cggcgctgct cgccgtgctg ggcgagaaca tcgacaagtg ggccgtacgg
    21781 cgcgcgcccg agccggaccg cgtcggcgtc tggttccgga tcgcgcagtg ggtcatgcgc
    21841 cgtccggtgc cgatcgccgc ggcggggacg ctggtgctgc tcgtgctcgg cgcgccgttc
    21901 ctcggcgtca agctggggct tccggacgac cgcgtgctgc ccccgggcgc gcccggtcgc
    21961 gtggtcaccg acgagctccg ccgggacttc gccggcaacg aggctgccgc gacgctcgta
    22021 ctcgcccccg gagtcgatcc ggtggccgac cgcgagcagg tgcggcgttt cgcacagagg
    22081 ctgtcctcga tcgaggggat agcgcgcgtc gacgcggcca cgggtagcta cgtcgctggc
    22141 gagctgctgt tcttctccga acgggtcggg cagcgtttcg cggccgacga cgcgacgtac
    22201 ttctcggcgg tcctcgcgcc cacggtcgag gcccagtcgc tagaggccga agaggtcgtc
    22261 cacggcatcc gggccctcga cgcgccgttc gcgttcgagg tcgccgggcc ctccgccgac
    22321 ctcgtcgacg gcaaggagag cgcgttcgcc cgcatcccga tcgcggtgct gtggatcgcg
    22381 ctcgtgacgt tcgtgaccct cttccttctg ttcgggagcg tgctcgtgcc tctcaaggcg
    22441 gtcgtgctca acctgttgag cctcaccgcc acgttcggcg cgatggtctg ggtcttccag
    22501 gacgggaacc tgtcgggcgt gctggacttc accgcgaccg ggacgatcgt gctgtcgaac
    22561 atcatcctga tgttctgcat agccttcggg ctctcgatgg actacgaggt cttccttctg
    22621 tcgcgcgtga aagaggagta cgacgcgacc ggggacaacg acctcgcggt cgcgcggggg
    22681 ctcgaccgca cgggacgcgt agtttccgcc g
//
LOCUS       DASQDO010000291         7729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_581801, whole genome shotgun sequence.
ACCESSION   DASQDO010000291 DASQDO010000000
VERSION     DASQDO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581801"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VEV43_08210"
     CDS             complement(<1..729)
                     /locus_tag="VEV43_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23542.1"
                     /translation="MIDPVLAHGVGRVYESPIPTYLYAVGAGLTVLLSFVLRALAGTK
                     DVTTRDKLIAGPRTARVLRGVLKVAALVALALTVAGAVLVDERGFTIAPLLFWVGLVV
                     GMTTLCALIGGAWEAADPWATLEGYYRIDDPERELMDPPWWAGPALIYALFWFELVSD
                     VGFEPAGILGALVVYSVLTFSLRHRLGERWGLVDPLSILFGFGSATSPLDLRDDGIHY
                     RGLGGIDRPDPMPRALVAAIVVLLA"
     gene            complement(726..1268)
                     /locus_tag="VEV43_08215"
     CDS             complement(726..1268)
                     /locus_tag="VEV43_08215"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HYP23543.1"
                     /translation="MFSKKLVVTCMVLGLAAGAAPAYAHGEIQGTDPAAKASVKRAPR
                     SVAITFTEAPTAQAVLDVTDGCKRDVGRGVDVTDATATVQVAGGQPGRWEVSYRVISA
                     LDGHLTRGAYTFTVAGRKDCTPDPKPTKEPDGPGEESAPPEAGDEDESEGSGVPVVPI
                     ALGAVALIAVAFVARRSGSG"
     gene            complement(1312..1878)
                     /gene="thpR"
                     /locus_tag="VEV43_08220"
     CDS             complement(1312..1878)
                     /gene="thpR"
                     /locus_tag="VEV43_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYP23544.1"
                     /translation="MGAEKLRLFTAVRVPREQLEWLDDAVGGLRPLSGARWTALDNQH
                     VTLNFLGWVESGLLGRVVAAMDEVANHHAPAEVSLTGLGAFPRERPARVLWAGIEDPA
                     GLLPSLAQDLGEGLRAVGYEPEERPYTAHLTLARFKTPRPMELPPIPAPPSPFTVDRV
                     TLYRSRLSPAGARYEVMHEAYLSAEGSQ"
     gene            complement(1868..3106)
                     /locus_tag="VEV43_08225"
     CDS             complement(1868..3106)
                     /locus_tag="VEV43_08225"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HYP23545.1"
                     /translation="MNAEVVAVGTELLLGQIANTNAQHISDALARVGVNVFFHTTVGD
                     NLVRMTDVIATALRRSDVVVITGGLGPTPDDVTREGVAAATGRALARDDRLVETIRGI
                     FAKLGRRSMPESNLRQADLPEGAKAIDPVGTAPGFELEHEGTLLFALPGVPWEMKAML
                     DSDVLPRLQAAAEGTIVSSEVLVVGVGESHVDEKISDLVATQSNPTIAYLAGGGRVKV
                     RLTAKAATEDAARTLIEPLEDEVRGRLGADAVGRGSASVAEDLGSMLRERGATVAVIE
                     SLTGGLIGAELTRLPGAGDFFTGGVVAYSNEVKDRVAGVDPDLLEEKGAVSEEVAAAL
                     ARSAAWTFGADLGLSATGVAGPGEQDGLPPGTVFVGASYRGRTEVRRPRAYGDRGNVR
                     AVTVTWAIDLGRRLLLDDGG"
     gene            complement(3103..3627)
                     /gene="pgsA"
                     /locus_tag="VEV43_08230"
     CDS             complement(3103..3627)
                     /gene="pgsA"
                     /locus_tag="VEV43_08230"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HYP23546.1"
                     /translation="MNLPNSITFARIALVPVFLVFAFGDSDAAQLTGLAVFVVASASD
                     SLDGYLARRHGSITTLGQFVDPLADKLLVGAALVVLVADEGFPLWAALVIAVREVAVQ
                     VLRIARTGGGRTRPASQFAKAKTATQLGMVSWWLLPWESTNAGHWAWIVAALLTTTVS
                     GFEYFARPPSKATA"
     gene            complement(3624..4970)
                     /gene="rimO"
                     /locus_tag="VEV43_08235"
     CDS             complement(3624..4970)
                     /gene="rimO"
                     /locus_tag="VEV43_08235"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HYP23547.1"
                     /translation="MSRVAIVTLGCPKNSIDSEGLGGMLDAAGHSVTDDPAAADVVVV
                     NTCGFIDPARRETVQEVLDLAELKHDGDLKGLVLTGCLVARSADDLAEALPEVDALVD
                     FAAYPRIDEIVRGAAGGTLLQRVFGDPGTKFDPAYWDATIARGPRIRFGRAPWAYVKI
                     AEGCDRGCTFCAIPLMRGKFRSRSTQTIVDEVRGLAAQGVSEISLVSQDSVMWGRDTG
                     EGGLLDLLAALERVDGLRRLRLMYLHPQGVTDELVDAMLASEVVAPYFDLSLQHVSPS
                     VLRAMGRWGGRDRFTRMIERIRSSEPLAAVRATFILGFPGEEDEDADEVEAFVGDTDL
                     DWIGTFTYSPEEGTRSFELDDRVPEAIARERAERVSAVAEQTMAERARSLVGTRLSVL
                     AERLDVATGTWTGRSHREAPEVDGEIAFTAAAPVSVGDYVDVEVTASDGADLVARA"
     gene            complement(5006..5776)
                     /locus_tag="VEV43_08240"
     CDS             complement(5006..5776)
                     /locus_tag="VEV43_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HYP23548.1"
                     /translation="MTEPTTIGAYLRAVRRRRRISIERAAEDTKIRAAFLMRMESDEF
                     DFLAPAYVRGFLRSYARYLEVDPGPLLEDFESRYGGGRVDSSQMIALQRREKAVPREH
                     RRLNSWVVAGGAAAVILVVLAFVGVMSAPEKPEPPGSALSQTTPTPGPAPSPSVSPSV
                     LPSESPGEIAATDGIELVVDAAGGASWLEVYADGQTVYYTTLPAGQTMTFDAEKRMHI
                     RLGYPAGVELTVNGRSLGSPGGEDPIELTFPDDLDGLI"
     gene            complement(5825..>7729)
                     /locus_tag="VEV43_08245"
     CDS             complement(5825..>7729)
                     /locus_tag="VEV43_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYP23549.1"
                     /translation="AGVTHLLQGNRSISAPLERLQRMGGIFGALIARPVSGLVGIWGT
                     WVLLLVLMFVGLLVVTRTPLSRVGGWIATGAAGAAGFVRGLASGHTGDDEEHDEDEDE
                     EEEEEEETYELEDEAGDEEGAEDDEAPAEVAAIKPAKPTQLAIPVGDTKSYRLPPLEV
                     LAKGGEREISTRSIEDTTRVLEGTLEQFNVDASVTGYTAGPTVTRFEIELGAGVKVNR
                     VLSLSNEIKYALASGELRFLAPIPGRSAIGVEVPNRSRQLVTLGDVLRSKEAQRDKHP
                     LAVALGQDISGETVLANVTEMPHVLIAGATNSGKSSCINSMITSILMRARPDQVRLIL
                     IDPKRVELTHFSGVPHLLTPVVTVPKKAASALGWVVREMEMRYETLAHSGMRNLEFYN
                     EAVARGAVVKRSDDDPDPEPLPYIMVVVDELSDLMMVAPRDVESAICRIAQMARAVGI
                     HLVVATQRPSVDVVTGVIKANIPSRIAFSVASQQDSRVILDQGGADKLIGRGDMLYLH
                     ANSSKPRRIQGSWVTEKEIAAVVGHARRQGDPDYQEGITAEEPGTGSAMGGGGGGDEL
                     LEEALQLVVRSQLGSTSMLQRKLQVGFARAGRLMDLLEQRGVVGPSQGSKPRDVLMTV
                     EELEARAESA"
BASE COUNT         1129 a         2837 c         2657 g         1106 t
ORIGIN      
        1 cgcaagtagc acgacgatcg ccgcgaccaa cgcgcgcggc atcgggtcgg gccggtcgat
       61 cccgccgagc ccccggtagt ggatgccgtc gtcgcgcagg tcgagcggag aggtcgccga
      121 cccgaacccg aacaggatcg acagggggtc gaccagcccc caccgctcgc ccagccggtg
      181 tcgcagcgag aacgtcagca cggagtagac gacgagcgcg ccgaggatgc cggcgggctc
      241 gaacccgacg tcggacacga gctcgaacca gaacaacgcg tagatgagag ccgggccggc
      301 ccaccacggc gggtccatca gctcgcgctc gggatcatcg atccggtagt agccctcgag
      361 cgtcgcccac gggtccgccg cttcccacgc gccgccgatc agcgcgcaca gcgtcgtcat
      421 cccgacgacg agcccgaccc agaacagcag cggcgcgatg gtgaacccgc gttcgtcgac
      481 gagcacggcg ccggcgaccg tgagggcgag cgccacgagg gccgcgacct tcaggacgcc
      541 tcgcagcacg cgtgcggttc gcggcccggc gatcagcttg tcccgggtgg tgacgtcctt
      601 ggtgcccgcc agggcacgca gcacgaacga cagcaggacg gtgagcccgg ccccgaccgc
      661 gtacaggtag gtcgggatcg gagattcgta gacgcgcccg acgccgtggg cgaggacggg
      721 gtcgatcatc ccgagccgga ccgccgtgcg acgaacgcga cggcgatcag ggcgacggca
      781 ccgagcgcga tcggcaccac cgggacgccg gacccctctg attcgtcctc gtcgcccgcc
      841 tccggcgggg cggactcttc gccgggaccg tccggctcct tcgtcggctt cgggtcgggg
      901 gtgcagtcct tccggccggc gaccgtgaac gtgtaggcac cgcgcgtcag gtggccgtcg
      961 agcgccgaga tcacgcgata cgagacctcc caccggccgg gctgtccccc cgcgacctgg
     1021 acggtcgcgg tcgcgtcggt gacgtcgacg ccgcgcccga cgtcgcgctt gcatccgtcc
     1081 gtcacgtcga gcacggcctg cgcggtcgga gcttcggtga acgtgatcgc gacggagcgg
     1141 ggcgcgcgct tcacgctcgc cttggcggcg gggtcggtgc cctggatctc gccgtgcgca
     1201 taggcaggcg cggcccccgc cgcgaggccc agcaccatgc acgtcacgac cagcttcttg
     1261 gagaacatcg tcgaccctct catcatcggc ctggataaca gaacgtcgtg gctactgcga
     1321 accttccgcc gagagatacg cctcgtgcat cacttcgtaa cgagcgccgg ccgggctcag
     1381 cctgctcctg tagagcgtga cgcggtcgac cgtgaacggc gacggcggcg ccgggatcgg
     1441 tgggagctcc atgggccgcg gcgtcttgaa gcgcgcaagc gtgaggtgcg cggtgtaagg
     1501 acgttcctcc ggctcgtaac cgacggcgcg gagcccttcg ccgaggtcct gcgcgagcga
     1561 gggcaggagc cccgcggggt cctcgatccc ggcccacagg acgcgcgccg ggcgctcgcg
     1621 cgggaacgcg cccaggcccg tcagcgagac ctccgcgggc gcgtggtggt tcgccacctc
     1681 gtccatcgcg gcaacgaccc ggccgaggag gcccgactcg acccagccga ggaagttcag
     1741 cgtgacgtgc tggttgtcga gcgccgtcca gcgcgcgccg ctcaggggcc ggagcccgcc
     1801 gaccgcgtcg tcgagccact cgagttgctc gcgcgggacg cgtaccgccg tgaagagccg
     1861 gagcttctca gcccccatcg tcgaggagca gccgccgccc gaggtcgatc gcccacgtca
     1921 cggtcacggc gcgcacgttg ccgcggtcgc cgtaggcgcg cggtcgccgc acctccgtcc
     1981 ggccgcggta cgacgcgccg acgaagacgg tgccgggggg aagcccgtcc tgctcgccgg
     2041 gccctgcgac accggtcgcc gacagaccga ggtccgcgcc gaaggtccac gcggccgagc
     2101 gggcgagggc cgcggccacc tcctcgctca ccgcgccctt ctcctccagc aggtcggggt
     2161 cgacgccggc gacgcggtcc ttcacctcgt tcgagtacgc gacgacgccg cccgtgaaga
     2221 agtcgcccgc gcccgggagc cgcgtcagct cggcgccgat cagccccccg gtgagcgact
     2281 cgatgaccgc gacggtcgcg ccgcgctcgc gcagcatcga gccgaggtcc tcggcgaccg
     2341 aggcgcttcc tcgtcctacg gcgtccgctc cgagccgtcc tcggacctcg tcctccagcg
     2401 gctcgatcaa cgtacgagcg gcgtcctcgg tcgccgcctt cgcggtgagc cgcaccttca
     2461 cgcggccccc tccggcgagg taggcgatgg tcgggttcga ctgggtcgcg acgaggtcgg
     2521 agatcttctc gtcgacgtgg gactccccca ccccgacgac caggacctcg ctgctgacga
     2581 tcgtcccctc ggccgcggct tgcaggcgcg ggaggacatc ggagtcgagc atcgccttca
     2641 tctcccaggg cacgccgggc agcgcgaaca gcagcgtccc ttcgtgctcg agctcgaagc
     2701 ccggcgcggt cccgaccggg tcgatcgctt tggccccctc ggggaggtcc gcctgccgca
     2761 ggttcgactc gggcatggac cggcggccca gcttggcgaa gatgccgcgg atcgtctcga
     2821 ccagacggtc gtcgcgggcg agcgcccgcc ccgttgccgc ggcgactccc tctcgcgtca
     2881 cgtcgtcggg cgtcggcccg agcccgcccg tgatcacgac cacgtccgac cgccgcaacg
     2941 cggtcgcgat cacatccgtc atccgcacga ggttgtcgcc gacggtcgtg tggaagaaga
     3001 cgttgactcc tacccgggcg agcgcgtccg agatgtgctg cgcgttcgtg ttggcgatct
     3061 ggccgaggag cagctcggtc ccgaccgcga cgacctccgc gttcaagcgg tcgccttcga
     3121 cgggggccgg gcgaagtact cgaaccccga caccgtcgtc gtcaggagcg cggcgactat
     3181 ccacgcccag tggccggcgt tcgtcgactc ccacgggagc agccaccacg acaccatccc
     3241 gagctgcgtc gcggtcttcg ccttcgcgaa ctgcgacgcg ggcctcgtcc ggcccccacc
     3301 ggtgcgggct atccggagca cctgaaccgc gacctcgcgc actgcgatca cgagcgcggc
     3361 ccacagcggg aacccctcgt cggcgacgag caccacgagc gccgccccga cgagcagctt
     3421 gtcggcgagg gggtcgacga actgacccag cgtcgtgatc gagccgtgtc tccgcgcgag
     3481 atagccgtcg agcgagtccg atgcggacgc gacgacgaac acggccagcc cggtcagctg
     3541 cgccgcgtcc gagtctccga acgcgaacac gaggaagacc ggtaccagcg ctatgcgcgc
     3601 gaacgtgatg gagttgggga ggttcacgcc ctggcgacga ggtccgcgcc gtcgctggcc
     3661 gtgacctcga cgtcgacgta gtcgccgacc gagacgggtg cggcagccgt gaacgcgatc
     3721 tcgccgtcga cctcgggggc ctcgcgatgc gacctcccgg tccacgtccc cgtggcgacg
     3781 tccagccgct cggcgaggac gctgagccgc gtcccgacga gcgaacgcgc ccgctcggcc
     3841 atcgtctgct ccgcgacggc cgagacacgt tcggccctct cgcgcgcgat cgcctcgggc
     3901 acgcggtcgt cgagctcgaa cgagcgggtc ccctcttcgg gcgagtacgt gaacgtcccg
     3961 atccagtcga ggtccgtgtc gccgacgaac gcctcgacct cgtccgcgtc ctcgtcctcc
     4021 tcgccgggga agccgaggat gaaggtcgcg cgcaccgcgg ccagaggctc cgacgaacgg
     4081 atgcgctcga tcatccgggt gaaccggtca cgccctcccc accggcccat cgcgcgcagc
     4141 acgctaggcg agacgtgctg cagcgagagg tcgaagtacg gcgcgaccac ctcgctggcg
     4201 agcatcgcgt cgacgagctc gtcggtgacg ccctgcgggt gcaggtacat gaggcgcagc
     4261 ctccggaggc cgtcgaccct ctcgagggcg gcgaggaggt cgagcagccc gccctcgccg
     4321 gtgtcgcgcc cccacatgac ggagtcctgg ctcacgagag agatctcgct cacgccctgc
     4381 gcggcaagcc cgcgcacctc gtcgacgatc gtctgcgtcg agcgcgaccg gaacttgccg
     4441 cgcatcagcg ggatcgcaca gaacgtgcag cctcggtcgc aaccctcggc gatcttcacg
     4501 taggcccacg gcgcgcggcc gaaccggatg cgcggccccc gtgcgatcgt ggcgtcccag
     4561 taggcggggt cgaacttcgt cccgggatcg ccgaagaccc tctgcaagag cgttcctccc
     4621 gcggcccccc ggacgatctc gtcgatccgc gggtatgcgg cgaagtccac gagagcgtcc
     4681 acctcgggca gcgcctccgc gaggtcgtct gcggaacggg cgacgaggca tcccgtcagc
     4741 acgaggccct tgaggtctcc gtcgtgcttc agctccgcga ggtcgaggac ctcctgcacg
     4801 gtctcacgcc gcgccgggtc gatgaagccg cacgtgttga cgacgacgac gtcggccgcg
     4861 gcgggatcgt ccgtgaccga gtgccccgcc gcgtcgagca tgcccccgag cccctccgag
     4921 tcgatggagt tcttcgggca gccgagcgtc acgattgcta cgcgggacac gtctatctcg
     4981 acgtcccgcc gaggcggccc gtcatctaga tcaggccgtc gaggtcgtcg gggaacgtga
     5041 gctcgatcgg gtcctcgccg ccgggcgacc cgaggctgcg gccgttcacg gtcagctcga
     5101 cgccggccgg gtagccgagc cggatgtgca tgcgcttctc ggcgtcgaac gtcatcgtct
     5161 ggccggcggg cagcgtcgtg tagtagacgg tctgtccgtc ggcgtagacc tcgagccacg
     5221 aagcacctcc ggccgcgtcg acgacgagct cgatgccgtc ggttgccgcg atctcgccgg
     5281 gagactccga cggcagcacg gacggcgaga cgctcggcga cggcgccggg ccgggcgtgg
     5341 gcgtcgtctg gctcagtgcg gagcccgggg gctcgggctt ctccggagcc gacatcacac
     5401 cgacgaacgc gaggaccacg aggatcaccg cggccgcacc gccggcgacg acccagctgt
     5461 tgaggcgacg gtgctccctc ggcacggcct tctcgcgccg ctgcaaggcg atcatctgcg
     5521 acgagtcgac gcggcccccg ccgtagcgcg actcgaagtc ctcgagaagg gggccggggt
     5581 cgacctcgag atagcgggcg tacgaccgga ggaacccgcg cacgtacgcg ggcgcgagga
     5641 agtcgaactc gtccgactcc atccgcatga ggaatgcggc tctgatcttc gtgtcctcgg
     5701 ccgctcgctc gatgctgatc cggcggcggc gccggacggc acggagataa gcgccgatgg
     5761 tggttggctc ggtcaccgcg ctcccgggtc gtgtgtgggg cgtcgattat aggagcgaac
     5821 ccccctacgc cgactcggcg cgcgcctcca gctcctcgac cgtcatcaac acgtcgcgcg
     5881 gcttggatcc ctgggagggc ccgaccactc cacgctgctc cagaaggtcc atcaggcggc
     5941 ccgctcgcgc gaacccgacc tggagcttgc gttggagcat cgaggtcgag ccgagctggg
     6001 agcggacgac gagctggagc gcttcctcca ggagctcgtc gccgcccccg ccgcctccca
     6061 tggcgctgcc cgttcccggc tcttcggcgg tgatgccctc ctggtagtcc gggtcgccct
     6121 gcctgcgagc gtggcccacg accgcggcga tctccttctc ggtcacccac gacccctgga
     6181 tgcgccgcgg cttcgacgag ttcgcgtgca ggtagagcat gtccccgcgc ccgatgagct
     6241 tgtccgctcc gccctggtcg aggatcacgc gcgagtcctg ctgcgacgcg acgctgaacg
     6301 cgatccggga cgggatgttc gccttgatca cgccggtgac gacgtcgacc gacgggcgct
     6361 gcgtagcgac cacgaggtgg atgccgacgg cgcgcgccat ctgggcgatg cggcagatcg
     6421 cgctctcgac gtcgcgcggc gcgaccatca tcaggtccga cagctcgtcg acgacgacca
     6481 tgatgtacgg caacggttcc ggatcggggt cgtcgtcgga acgcttcacg acggcccctc
     6541 gggcgacggc ctcgttgtag aactcgaggt tccgcatccc ggagtgcgcg agcgtctcgt
     6601 agcgcatctc catctcacgc acgacccagc cgagcgcgga cgccgccttc ttcggaaccg
     6661 tgacgaccgg cgtcaggagg tgcggcactc cggagaagtg cgtcagctcg accctcttgg
     6721 ggtcgatcag gatcagacgc acctgatcgg gccgcgcgcg catgaggatc gacgtgatca
     6781 tcgagttgat gcacgacgac ttgccggagt tcgtggcgcc ggcgatcagc acgtgcggca
     6841 tctcggtcac gttcgcgagc acggtctctc cggagatgtc ctgcccgagc gccacggcga
     6901 ggggatgctt gtcgcgctgc gcctccttgc tgcgcagcac gtcgcccagc gtgacgagct
     6961 gacggctccg gttcggcacc tcgacgccga tggccgaccg cccggggatg ggggccagga
     7021 aacgcagctc gcccgacgcg agggcgtact tgatctcgtt gctgaggctc agcacgcggt
     7081 tgaccttcac cccggcaccg agctcgatct cgaagcgcgt gaccgtcggc cccgcggtgt
     7141 agccggtgac ggacgcatcg acgttgaact gttcgagcgt cccctcgagc acccgcgtcg
     7201 tgtcctcgat cgaccgcgtc gatatctcgc gctcgccgcc cttcgccagc acctcgagcg
     7261 gcggcaggcg atacgacttc gtgtccccga ctgggatggc gagctgcgtc ggcttcgcag
     7321 gcttgatcgc ggcgacctcg gcgggagcct cgtcgtcctc ggccccctct tcgtctccgg
     7381 cctcgtcttc gagctcgtag gtctcttctt cttcttcttc ttcttcgtcc tcgtcctcgt
     7441 cgtgctcctc gtcgtcgccg gtgtgccccg acgcgagccc gcgcacgaac ccggccgccc
     7501 ccgcggcccc cgtggcgatc caccctccga cccggctgag cggcgtgcgc gtcaccacga
     7561 gcagcccgac gaacatcagg accagcagca ggacccacgt cccccagatc ccgacgagcc
     7621 ccgacaccgg acgggcgatc aacgcgccga agatcccgcc catgcgctgg agccgctcga
     7681 gcggcgccga gatcgagcgg ttgccctgaa ggaggtgtgt cacgccggc
//
LOCUS       DASQDO010000292        10440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1071575, whole genome shotgun sequence.
ACCESSION   DASQDO010000292 DASQDO010000000
VERSION     DASQDO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071575"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VEV43_08250"
     CDS             <1..692
                     /locus_tag="VEV43_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYP23550.1"
                     /translation="ATLSAIRRFWTEESVSIDSPFFTIREARSLPRPTQRPHPPLWGV
                     AARARQSFAWLGENGLGLMGALTSQGPDVLREQIDIYRDASRTAGHGDGEVVILVSLY
                     VAPDHSTAIREGDHYLEAYHRVWASAASAWEHMSSGAYPGYEGMARSIRSSTPAVMRK
                     ARAVAFGSPAAVAEYVQWVDEKLGVDEVLWNLDFGAMPGGLARRSLELFLEEVLPQVS
                     ADPVEQAAGLA"
     gene            complement(752..1909)
                     /locus_tag="VEV43_08255"
     CDS             complement(752..1909)
                     /locus_tag="VEV43_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23551.1"
                     /translation="MKKFLVALACAGLVASGLSATAGAGPSPGGYSSDNVEWVTHVPF
                     NGPTATGANFFKSGGDHYMIVTSWHNFAIYNINDPLAPELVGNPVPFGFKFENEDVST
                     NGKIMLFSETIPQGDLHVWDIEDKSNPVLLATVAGAGDHTTTCILKCKWAYGSEGTIT
                     DLRDPANPVLMTEKWSDNQPPMGSTHDVTEVAPGVVLTSTAPMLLLDARKNPVKPKFL
                     ALTEPYEGNGLPFHSNLWPRNMKDRWIIAGGETCCGAEQCDESTAAAFMVWDTKGWQK
                     SRTFKLVDEWWAYQGTVIDGGGIASAPFGCSPHWFTTQPGTWKNGGLVAVGWYNSGTR
                     LLEVSSKGKISEAGWFLPAGGGTSGAYFINEEIIYAVDYQRGIDVLRYTGK"
     gene            2416..3552
                     /locus_tag="VEV43_08260"
     CDS             2416..3552
                     /locus_tag="VEV43_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23552.1"
                     /translation="MRDKRARLLALLTGAVLVGAMATPATAQTPVTFEILTGAPVPGT
                     QAFDFHMFPSELTVHPGDTLHFDWGGVALLPVGTTDSPFGGFFAADPDDDGFKLDGRM
                     FFPTPAGTNCGLRAKKPCTYDGSALLYPGEQNFKNGFYVKIADSLDDATFTVTNTFHT
                     LTVTVSSAAQQSPTSDPNLDSHLAQSAQEAEDLLEMLNEVHYETTEDGKRIYTAYMGY
                     DTEHITFFEAFPQNLRIRKGDQIQWIFAQNVVEPHTATTPPAKAKRWANVAFNSPPVC
                     DPDGDEGPGPDQPADFENPTFCDDPSMAELDLPNKFAKEQGDGKVPTGGDRDKDYDTS
                     GLRGGPEGIPDDPYALRFTRKSGDTPFTVMCAIHWNMKTKVTVR"
     gene            3674..4975
                     /locus_tag="VEV43_08265"
     CDS             3674..4975
                     /locus_tag="VEV43_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HYP23553.1"
                     /translation="MELRKMLVGGLMAALVAGAIAPAGASGSEPPRTEFEEGEGAAWT
                     THEGELEFLAAVDEASDRVEIDVIGKTKQGRPLHLVRLGYPTPASRFAALKRPTTLFV
                     CSQHGNEPAGREACLQRLRDLAFTDDAELVTQLKEQTILFVPSANPDGREADARGNSD
                     GTDINRDHLNLEASESRAMAKVVRDWQPEAVVDLHEYGPSLPALYDDEVLVLWPRNLN
                     VDEDVHAQAKTLALDYITADEEEAGYSTDEYGQYELGDQDVHQSAGDGDEGIARNAMG
                     LRHSLGILVETRVDGDPRNGPQELDDPAVRMRRVAAHEVAVDATLRFMRERGAAVAGV
                     TKQARRRAVKEGRLRNEPVYFGGADNAEPSENQVQDPPPCGYLLSKKQYRQVRRTLSL
                     LGIKARHMYVSMAQAAEPLIPLLLDERGTRHSVPAKPEADC"
     gene            complement(4972..5523)
                     /locus_tag="VEV43_08270"
     CDS             complement(4972..5523)
                     /locus_tag="VEV43_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23554.1"
                     /translation="MRRPVPVPVWQLVAGAVACCVLGWFAFVRGTNVPVLALADLGFH
                     ELGHLLTYVFPDVVTAMMGSITQVAVPWGLAAYFFVRRGDFLGGTFCLAWAGTAAHNA
                     SVYVADAPWQTLELIGGSHDWAFEFGPYDAQDRAADVAGIVRGAGLAMLLTALAICLG
                     GLVLATGSRARSPLRKGRAVTGA"
     gene            5625..6377
                     /locus_tag="VEV43_08275"
     CDS             5625..6377
                     /locus_tag="VEV43_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HYP23555.1"
                     /translation="MGVWSRILTLFRIKANKALDRAEDPRETLDYSYEKQVDLLQEMR
                     RGIAEVATARKRIELQQRQLQQSAGRLTTQAQQALQTNREDLARQALTRRVAIESELG
                     ELQVQRDQLHEEEQKMIEASRVLENKLATMRTRKETMKASYSAAEAQVRVGEAATGIS
                     KEMGEVMRMMRRAEDRVAEMQARSGALEELTSSGALEDLSVSGDRIQAELDVAVAQAQ
                     VDAEMAKMKRQLPAAQPGLTAPTETEKSREQR"
     gene            complement(6425..6955)
                     /locus_tag="VEV43_08280"
     CDS             complement(6425..6955)
                     /locus_tag="VEV43_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23556.1"
                     /translation="MGEIDEAALVVLGAVLALLGAWLADRRKVEHQRATRWIERRREV
                     YSSFIHSAREIFHSLERFLPDGPIGDNVASVEQRWRELEETYSDFLLIASESTFQVAA
                     EVRAWCENAAKLLVASEPDEAAIKRHMQFFWAPAAAFLDRCRADVESPRVISIWHRNA
                     QDKSVSGSEPNPYVAP"
     gene            complement(7055..8512)
                     /locus_tag="VEV43_08285"
     CDS             complement(7055..8512)
                     /locus_tag="VEV43_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYP23557.1"
                     /translation="MLYVGAAVLGGLLLLIGIFKLMWRVAEPNEALIISGLREHEKPG
                     DVGESLGFKIVTGRGTLVIPGVQTVRRLSLDLREAELAIDCVTHQGIPLAIRGVVIFK
                     VGDDFVSIANAARRFLDQQDQMDARVHNVFAGHLRAIVGNMTVEEMIRDREKLTQLTR
                     GSSGTEMEKLGLIVDSLQIQEIGDPTGYIKNLGRPHAAAVERDARIAQAGADRAATEK
                     EQEADALKAHARRDSSIKQAGYQAEVDTASAQAAQAGPLADAGARQEVVVQETKVAEL
                     EAQREEQRLQATVRKPADARAYEQTTLAKAERDAQISGAEAEAQRVKLEAEAEAERVR
                     QAASAEAEHVRLESAARAESTQIIGEAEASATRAKGLAEAEAVRAKGMAEAETIAARA
                     DALAENQDAVIGQQLAENWPAIVEAAAKPFGGIDQLIVLNGAQGLSETLAQALSQGVA
                     GLELARNVLSKGKGGNGGNGTDEREPSAPARRSGS"
     gene            complement(8543..8737)
                     /locus_tag="VEV43_08290"
     CDS             complement(8543..8737)
                     /locus_tag="VEV43_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23558.1"
                     /translation="MVGKVGRVTSRIDGTHPGEVMVPVRGGSEAFNAYASDEEGAIPT
                     GTRVVVVEYFPPRTVIVSRA"
     gene            complement(8815..9459)
                     /gene="msrA"
                     /locus_tag="VEV43_08295"
     CDS             complement(8815..9459)
                     /gene="msrA"
                     /locus_tag="VEV43_08295"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYP23559.1"
                     /translation="MFSLRKKTQMPEPSDALKGRDERPFPIPSTHAVLGTPLEPPFPE
                     GMEVAVFGLGCFWGAERLFWTQDGVYSTAAGYAGGFTPHPTYEEVCSGMTGHTEVVQV
                     VFDPSKVSYADLLKVFFEEHDPTQGMRQGNDVGTQYRSAIYYTNDDQKRVAAEAIRSY
                     DEALRGRGFDPVTTEVAAAGPFYYAEDYHQQYLHKVPDGYCSLKGTGVACAIPR"
     gene            complement(9475..>10440)
                     /locus_tag="VEV43_08300"
     CDS             complement(9475..>10440)
                     /locus_tag="VEV43_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23560.1"
                     /translation="AGEGFTSEIENVSGTGYSDLLRGDDGPNVLFGGDVSAGFFGPTY
                     PPGGVSDTLEGRGGDDTIDGGTGDDTIDGGGDDDSIRDGRGRDVIDAGAGDDFVTTAF
                     RCGGGPDEDRCSRPQEAYLGDDVEGGDGFDILTGGFGDDLLSGGVGVDHLAGGDGVDD
                     LRGGEGSDTLRGGDGRDALDGGAGSDMADFADAPAGVTVDLGAQTVTGGSDDTMTSME
                     DAAGSAFDDVLLGTDEANGLDGRDGDDVLRGLGGDDYATGNDGEDEVYGGEGDDEIEG
                     GSGPHYLGHNEADGRPDLQDGGPGEDFCFGSNHDELVSCEIIAVE"
BASE COUNT         1633 a         3690 c         3519 g         1598 t
ORIGIN      
        1 aagcgacgct ctcggcgatc cgcaggttct ggacggaaga gagcgtctcg atcgactctc
       61 cgttcttcac catccgcgaa gcgcgcagcc tcccgcgtcc gacgcaacgg ccgcatccac
      121 ctctgtgggg ggtcgcggcg cgagctcgtc agtcgttcgc atggctcgga gagaacggcc
      181 tcggcctgat gggcgcgctg accagccagg gccccgacgt cctgcgcgag cagatagaca
      241 tctaccgcga tgcttctcgc actgccggcc acggtgacgg cgaggtggtg atcctcgtct
      301 cgttgtacgt cgcgcccgac cacagcacgg cgatccggga gggcgaccac tatctggagg
      361 cgtatcaccg ggtctgggcc tccgcggctt ccgcctggga gcacatgtcg tcgggcgcgt
      421 acccgggcta cgaagggatg gcccggagca tccgctccag cacgccggcg gtcatgcgca
      481 aggcccgggc cgtcgctttc gggagcccgg ccgcggtggc cgagtacgtc cagtgggtcg
      541 acgagaagct cggggtggac gaggtcttgt ggaacctcga cttcggcgcg atgcccggag
      601 gcctcgcgcg ccgctcgctg gagctgttcc tcgaggaggt gctgccgcag gtgagcgcag
      661 accccgtgga gcaagcggcc gggctggcct gacctcggct gaacgcgaag aggcccgccc
      721 cgaagggcgg gcctctcgac aaacgactga gctacttgcc ggtgtagcgc aggacgtcga
      781 tgccccgctg gtagtcgacg gcgtagatga tctcctcgtt gatgaagtag gcgcccgagg
      841 tgccaccgcc ggccgggagg aaccagccgg cctcgctgat cttgcccttc gagctcacct
      901 cgaggagccg ggtgccgctg ttgtaccaac cgactgcgac gaggccgccg ttcttccagg
      961 tgcccggctg cgtcgtgaac cagtgtggtg aacatccgaa tggagcgctg gcgatgcccc
     1021 caccgtcgat caccgtcccc tggtacgccc accactcgtc gacgagcttg aaggtgcggc
     1081 tcttctgcca gcccttcgtg tcccacacca tgaacgcggc ggccgtggac tcgtcgcact
     1141 gctcggcgcc gcagcaggtc tcgccgccgg cgatgatcca gcggtccttc atgttccgcg
     1201 gccacaggtt cgagtggaac gggagcccgt tgccctcgta cggctcggtc agggcgagga
     1261 acttcggctt gaccgggttc ttgcgggcgt cgaggagcaa catcggcgcg gtcgacgtca
     1321 gcacgacgcc cggcgccacc tcggtgacgt cgtgggtcga gcccatcgga ggctggttgt
     1381 cgctccactt ctccgtcatg aggaccgggt tcgcggggtc acgaaggtcg gtgatcgtcc
     1441 cttcgctgcc gtacgcccac ttgcacttga ggatgcacgt ggtggtgtgg tcgccggccc
     1501 cggcgaccgt cgcgagcagg accgggttgg acttgtcctc gatgtcccac acgtggaggt
     1561 cgccctgggg gatggtctcg gagaacagca tgatcttgcc gttcgtcgag acgtcttcgt
     1621 tctcgaactt gaagccgaac ggcaccgggt tgcccacgag ctcgggggcg agcgggtcgt
     1681 tgatgttgta gatcgcgaag ttgtgccagc tggtcacgat catgtagtgg tcgccgccgc
     1741 tcttgaagaa gttggcgccg gtcgcagtgg ggccgttgaa cggcacgtgg gtcacccact
     1801 cgacgttgtc cgacgagtag ccgccggggc tggggccggc accggcggtt gcgctcagcc
     1861 cggaggcgac cagccccgcg caggccaggg ccacgagaaa ctttttcacg atcttcctcc
     1921 tgttttggga cgtctgggag aggtttcgcc ctcctcggtc tggttcctcc cagacgttgt
     1981 ggggcggccg cgcggcgtcg gtgcgagtgg gtggagggcg cgtcggtcag gagctccggc
     2041 tcggcgtcgg ggtccgggtc gggctccgcc tcgggggtcg cctcgggcag cggctcggcc
     2101 acgggcgtcg cgaccggcga ggccgcctgc accgagttcg gggcgggccg cctcgcggac
     2161 gcctccctcg acgtcgtgct cgacgagtgg gaggtcgacc cgaggcggag agcagcgcgg
     2221 ccgggacgat cgagttcgag ctgacgaacg ccggcgagac cgagttcgtc gtcgagggct
     2281 gaggtagctc accgcgcgca gcgacaagat acgtacaaga cgcaggcatt cgtcctggtc
     2341 cgtcctcatg tgaattggtg tcatggccct cgtgccctcg gggcaccgga cgaacgaccc
     2401 caaggagggc gatacatgag ggacaagaga gcgcggctcc tcgcgctgtt gaccggtgcg
     2461 gtcctcgtcg gcgcgatggc gacgccggct accgcacaga caccggtcac cttcgagatc
     2521 ctgaccggcg ccccggttcc cggcacgcag gcgttcgact tccacatgtt cccgagcgag
     2581 ctcacggtcc acccgggcga caccctgcac ttcgactggg gtggcgtcgc gctgctgccc
     2641 gtcggcacga ccgactcgcc gttcggcggg ttcttcgcgg ccgacccgga cgacgacggc
     2701 ttcaagctcg acggcaggat gttcttcccg acaccggcgg ggacgaactg cggcctgcgg
     2761 gcgaagaagc cgtgcacgta cgacgggtcc gcgctgctgt atccgggcga gcagaacttc
     2821 aagaacgggt tctacgtgaa gatcgccgac tcgctcgacg acgcgacgtt caccgtcacg
     2881 aacacgttcc acacgctgac ggtgaccgtg agctccgccg cgcagcagtc ccccaccagc
     2941 gacccgaacc tcgactcgca cctcgcgcag tccgcacagg aggccgaaga ccttctcgag
     3001 atgctgaacg aggtccacta cgagaccacc gaggacggca agcggatcta cacggcctac
     3061 atgggctacg acaccgagca catcactttc ttcgaggcgt tcccgcagaa cctgcggatc
     3121 cgcaagggcg accagatcca gtggatcttc gcccagaacg tggtcgagcc tcacaccgcg
     3181 accacgccgc cggcgaaggc gaagaggtgg gccaacgtgg ccttcaacag cccgcccgtc
     3241 tgcgaccccg acggcgacga gggcccgggc ccggatcagc ctgcggactt cgagaacccg
     3301 acgttctgcg acgacccgtc gatggccgag ctcgacctgc ccaacaagtt cgccaaggag
     3361 cagggtgacg gcaaggtacc gacgggcggt gaccgggaca aggactacga cacgtcgggc
     3421 ctgcgcgggg ggccggaggg catccccgac gacccgtacg cgctgaggtt cacgcgcaag
     3481 tccggcgaca cgccgttcac cgtgatgtgt gcgatccact ggaacatgaa gacgaaggtg
     3541 acggtcaggt aaccactgtc cggaggagag cggccccccg aagggggccg ctttcgcgcc
     3601 cgggtgaaaa cagggtctcc ccggggccgc gtcgaagtcc tgccgtgaga ggggctcgag
     3661 gaggaggcag gcagtggaac tcaggaagat gctcgtaggc ggcctgatgg cggccctggt
     3721 ggcgggtgcg atcgcaccgg cgggcgccag cggatcggag ccgccccgga ccgagttcga
     3781 agagggcgaa ggcgccgcct ggaccaccca cgagggcgag ctcgagttcc tcgccgccgt
     3841 cgacgaggcc tcggaccgcg tcgagatcga cgtgatcgga aagaccaagc agggccggcc
     3901 gctgcacctc gtgcgcctcg gctaccccac gccggcgtca cgcttcgccg cgctgaagcg
     3961 ccccacgacg ttgttcgtct gctcccagca cggcaacgag cccgccggcc gggaagcctg
     4021 cctccagcgc ctgcgcgacc tcgcgttcac cgacgacgcc gagctcgtga cgcagcttaa
     4081 ggagcagacg atcctgttcg tgccgagcgc gaaccccgac gggcgcgagg ccgacgcgcg
     4141 cgggaactcc gacggcacgg acatcaaccg ggaccacctc aacctcgagg cctccgagag
     4201 ccgagcgatg gcgaaggtcg tgcgcgactg gcagccggag gcggtcgtcg acctccacga
     4261 gtacgggccc tctctgccgg ccctctacga cgacgaggtg ctcgtgctgt ggccgcgcaa
     4321 cctgaacgtc gacgaggacg tccacgctca ggcgaagacg ctcgcgctcg actacatcac
     4381 cgcggacgaa gaggaagcgg gttactcgac cgacgagtac ggccagtacg agctcggcga
     4441 ccaggacgtc caccagtcgg ccggcgacgg cgacgagggc atcgctcgca acgcgatggg
     4501 gctgcggcac tcgctgggca tcctcgtcga gacccgcgtc gacggcgacc ctcgcaacgg
     4561 cccgcaggag cttgacgacc cggccgtccg catgcgccgc gtggcggccc acgaggtcgc
     4621 ggtcgacgcg acgctccggt tcatgcgcga gcgcggagcc gcggtcgcgg gcgtgacgaa
     4681 gcaggcgcgc cggcgcgcgg tgaaggaggg acggctgcgc aacgagcccg tctacttcgg
     4741 cggcgccgac aacgccgagc cgtccgagaa ccaggtccag gacccgccgc cgtgcggcta
     4801 cctgctgtcg aagaagcagt accggcaggt gcgccggaca ctgtcactgc tggggatcaa
     4861 ggcgcggcac atgtacgtgt cgatggctca ggcggccgag ccgttgatcc cgctgctcct
     4921 cgacgagcgg gggacgcggc attcggtacc ggccaagccc gaggcggact gctaggcgcc
     4981 cgtcaccgcc cggccttttc ggagcgggct acgagctcgc gagccggtag ccagcacgag
     5041 tccccccagg cagatcgcga gtgcggtcag cagcatcgcg aggcccgcgc cgcgcacgat
     5101 ccccgcgacg tccgccgcgc ggtcctgcgc gtcgtagggc ccgaactcga acgcccagtc
     5161 gtggctaccg ccgatgagct cgagggtctg ccacggcgcg tcggcgacgt agacgctcgc
     5221 gttatgcgcc gccgtgccgg cccacgcgag gcagaaagtc cctcctaaga agtccccccg
     5281 ccggacgaag aagtacgcgg cgaggcccca cggcaccgcc acctgcgtga tcgaccccat
     5341 catcgccgtc acgacgtccg ggaagacgta cgtgagcagg tggccgagct cgtggaagcc
     5401 gaggtcggcc agagccagga ccgggacgtt cgtgccgcgt acgaacgcga accagccgag
     5461 gacgcagcac gcgacggcgc ccgcgacgag ctgccagacg ggtacgggga ccgggcgtct
     5521 catgcttgcc ttatcggcgc gtaacggggt tcggacgagc ccctgacgta tgctggcccc
     5581 gcagggacga gcccccagac gcaggtggac aaggagattt cggcatgggc gtgtggtcgc
     5641 ggattctgac cctcttcagg atcaaggcga acaaggccct cgacagggcc gaggatcctc
     5701 gcgagacgct cgactactcc tacgagaagc aggtcgacct gctccaggag atgcgccgcg
     5761 ggatcgcgga ggtcgcgacc gcgcgcaagc gcatcgagct ccagcagcga cagctccagc
     5821 aatcggcggg gcggctgacg acgcaggccc agcaggcgct ccagaccaac cgcgaggacc
     5881 tggcgcgcca ggcgctgacg cgcagggtcg cgatcgagtc cgagctcggt gagctccagg
     5941 tccagcgcga ccagctccac gaggaagagc agaagatgat cgaggcgtca cgcgtgctcg
     6001 agaacaagct cgcgaccatg cggacgcgca aggagacgat gaaggcgtcc tactccgcgg
     6061 cggaggccca ggttcgagtc ggtgaggccg ctaccggcat ctcgaaggag atgggcgagg
     6121 tcatgcggat gatgcgccgc gccgaggacc gcgtcgccga gatgcaggcc cggtccggcg
     6181 cgctcgagga gctgacgtcc tccggcgcgc tcgaagacct gtcggtgtcg ggcgaccgca
     6241 tccaggccga gctcgacgtc gcggtagcgc aggcccaggt cgacgccgag atggcgaaga
     6301 tgaagcgtca gctaccggcg gcccagccgg gcctgacggc gccgacggag accgagaagt
     6361 cgcgcgaaca gcgctgacgc gctaggaacc gctgggctcg cgcacacgcc tctgatcgac
     6421 cctgctacgg cgcgacgtat ggattcggct ctgacccaga cacacttttg tcttgagcgt
     6481 tccgatgcca gatggatatg acgcgaggac tctccacatc ggctcggcat ctgtctagaa
     6541 atgccgcagc gggcgcccag aagaactgca tatggcgttt gattgccgct tcgtcgggct
     6601 ccgaggcgac taggagtttc gctgcgttct cacaccaggc ccgaacctcc gcagccactt
     6661 ggaatgtgga ttcagaggct atgaggagaa agtccgagta ggtttcctcg agctcgcgcc
     6721 acctctgctc gacgctagcg acgttgtcgc ctatcggtcc atcagggagg aacctctcga
     6781 gactgtggaa gatctctcta gctgagtgga tgaacgagct atagacctcg cgtcgacgtt
     6841 ctatccacct ggttgctcgc tgatgttcga ccttccgccg atcagctagc cacgcgccga
     6901 ggagcgccaa gaccgccccc aggacaacca aagcggcttc gtcgatctct cccatgtacg
     6961 tcgcttaccc caacgcacga agaggatgct gagcccgacc cgcgtccccc cccagagcta
     7021 gtccgcaccg tcgtcacgcc caggcgccga cgcgctagga accgctcctc cgcgcggggg
     7081 ccgagggctc ccgctcgtcg gttccgttcc ctccgttccc gcccttcccc ttcgacagga
     7141 cgttgcgcgc cagctccagc ccggcgaccc cctggctcag cgcctgggcc agcgtctccg
     7201 acagcccctg cgccccgttc aggacgatca gctggtcgat gccgccgaac ggcttcgccg
     7261 cggcctcgac gatcgccggc cagttctccg caagctgctg cccgatgacc gcgtcctggt
     7321 tctccgccag cgcgtcggcg cgggccgcga tcgtctcggc ctcggccatg cccttggcgc
     7381 gtacggcctc cgcctcggcc aggcccttcg cgcgggttgc cgacgcctct gcctcgccga
     7441 tgatctgggt cgactcggcc cgcgccgccg actcgagccg gacgtgctcg gcttccgccg
     7501 acgccgcctg gcggacgcgc tcggcctccg cctcggcttc tagcttcacg cgctgcgcct
     7561 cggcctcggc gcccgagatc tgcgcgtcgc gctcggcctt cgccagcgtc gtctgctcgt
     7621 aggcgcgtgc gtcggcgggc ttgcggaccg tcgcctgcag acgctgctcc tcgcgctgcg
     7681 cctcgagctc ggcgaccttc gtctcctgga cgacgacctc ctggcgcgcg ccggcgtcgg
     7741 caagcggccc cgcctgcgcg gcctgcgcgc tggcagtgtc gacctcggcc tggtaacccg
     7801 cctgcttgat cgagctgtcg cggcgagcgt gcgccttgag cgcgtccgcc tcctgctcct
     7861 tctcggtagc ggcgcggtcg gccccggcct gggcgatcct cgcgtcgcgc tcgacggccg
     7921 cggcgtgcgg gcgcccgagg ttcttgatgt agcccgtggg gtcgccgatc tcctggatct
     7981 gcaacgagtc gacgatgagg ccgagcttct ccatctcggt gcccgacgat ccgcgcgtca
     8041 gctgcgtgag cttctcgcgg tcgcggatca tctcctcgac cgtcatgttc ccgacgatcg
     8101 cgcggaggtg accggcgaac acgttgtgga cgcgcgcgtc catctgatcc tgctggtcga
     8161 ggaaacgacg ggccgcgttc gcgatcgaga cgaagtcgtc gccgaccttg aagatgacga
     8221 cgccgcggat cgcgagcggg atgccctggt gcgtcacgca gtcgatcgcg agctcggcct
     8281 cgcgcaggtc gagcgacagc cggcggaccg tctgcacgcc cgggatcacg agcgtgcctc
     8341 gtccggtcac gatcttgaag ccgaggctct cgcccacgtc acccggcttc tcgtgctcgc
     8401 gcagacccga gatgatcagc gcctcgttcg gctcggccac ccgccacatc agcttgaaaa
     8461 tgccgatgag cagcaacagc ccacccagca ccgcggcccc cacgtacagc atcacgccac
     8521 cccctaacgg cacctctgcg ccctaagcac gactaacgat cacggttcgc ggagggaagt
     8581 actccacgac gaccacgcgg gtaccggtcg ggatcgcgcc ctcctcgtcg gaggcgtacg
     8641 cgttgaaggc ttcgctccca ccccggacgg ggaccatcac ctcgcccggg tgggtgccgt
     8701 cgatgcggct cgtgacacgg cccaccttcc ccaccatcct ctgatccgcc actcacacct
     8761 cgtgtgtcta gacgacctcg atcgcgattg tcacacggcc cccctgcggg cgggctagcg
     8821 cgggatcgcg caggcgacgc cggtgccctt caggctgcag tagccgtcgg gcactttgtg
     8881 caggtactgc tggtggtaat cctccgcgta gtagaagggg cccgcggcgg ccacctcggt
     8941 cgtgacgggg tcgaacccgc ggccgcgcag cgcctcgtcg taggaacgga tcgcttccgc
     9001 agcgacccgc ttctggtcgt cgttcgtgta gtagatcgcc gagcggtact gcgtgccgac
     9061 gtcgttgccc tgccgcatcc cctgcgtcgg gtcgtgctct tcgaagaaca ccttcagcag
     9121 gtcggcatac gagaccttcg agggatcgaa cacgacctgg acgacctcgg tgtgcccggt
     9181 catcccgctg cacacctctt cgtaggtcgg gtgcggcgtg aagccgcccg cgtacccggc
     9241 cgccgtcgag tagacgccgt cctgagtcca gaaaagacgc tcggcccccc agaagcagcc
     9301 gagcccgaac accgcgacct ccatcccctc cgggaagggg ggctcgagcg gcgtgccgag
     9361 caccgcgtgc gtcgacggga tcgggaaagg ccgctcgtcc cggcccttca gggcgtcgga
     9421 cggctcgggc atctgcgtct tcttcctgag agagaacatg tatccagtat cgctctactc
     9481 gaccgcaatg atctcgcagg agacgagttc gtcgtggttg ctgccgaagc agaagtcctc
     9541 gcccggcccc ccgtcctgca ggtcgggacg cccgtcggcc tcgttgtgcc cgaggtagtg
     9601 ggggccgctc ccgccctcga tctcgtcgtc gccctcgccg ccgtagacct cgtcctcgcc
     9661 gtcgttcccc gtcgcgtagt cgtcgccgcc gaggccccgc aggacgtcgt caccgtcgcg
     9721 cccgtcgagg ccgttcgcct cgtcggtgcc gagcagcacg tcgtcgaacg ccgagcccgc
     9781 tgcatcctcc atcgacgtca tcgtgtcgtc gctcccgccg gtgacggtct gcgccccgag
     9841 gtccaccgtc acacctgccg gagcgtccgc gaagtcggcc atgtcggacc ccgcgcctcc
     9901 gtcgagcgcg tcgcggccgt cacccccgcg cagggtgtcg gaaccctcgc cgccgcgcag
     9961 gtcgtcgacg ccgtcgccac ccgcgaggtg atccacgccc actccgcccg acagcaagtc
    10021 gtcgccgaag ccgccggtca agatgtcgaa cccgtcgccg ccctccacgt cgtcgccgag
    10081 gtacgcctcc tggggccgcg agcaccggtc ttcgtccggg ccacctccgc atcggaacgc
    10141 ggtcgtgacg aagtcgtctc ccgcacccgc atcgataacg tcgcggccgc gcccgtcgcg
    10201 gatcgagtcg tcgtctccgc cgccgtcgat cgtgtcgtcc ccggtgcctc cgtcgatcgt
    10261 gtcgtcgccg ccccggccct ccagcgtgtc ggacacgccg cccggcggat aggtcggccc
    10321 gaagaaccct gccgagacgt cgccgccgaa caggacgttc ggcccgtcgt cgccgcgcag
    10381 caggtcggag tagccggtgc ccgagacgtt ctcgatctcc gaggtgaagc cctctcccgc
//
LOCUS       DASQDO010000293         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_92375, whole genome shotgun sequence.
ACCESSION   DASQDO010000293 DASQDO010000000
VERSION     DASQDO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92375"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VEV43_08305"
     CDS             <1..866
                     /locus_tag="VEV43_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HYP23561.1"
                     /translation="GEYDVPLVLQDRTFRRDGSFKFRDSHDDVLGEVFLVNGRPMPVL
                     EVANRKYRFRILNASNSRGYQLALSTGEPLIQVASDQGLLSAPTPSAKIPLWTAERAD
                     VVVDFSKYPVGTRVVLGDRQDDADPAGAAPLMAFDVTREEADDSSLPAMFRPIQRILP
                     VPGTIEREFALEFDFDRQLWQINGKVFDPNRIDVKPRLGDTEIWTFRNLSNVTHPMHV
                     HLVRFQVLDRDGLPPSPNEMGWKDTVRVGQGEVVRVAMTFEGHTGRYMFHCHNLAHED
                     HSMMGQMKVVD"
     gene            complement(897..1025)
                     /locus_tag="VEV43_08310"
     CDS             complement(897..1025)
                     /locus_tag="VEV43_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23562.1"
                     /translation="MSRRVRLVVMATLYATILAAAPAEAEECVEVLQFKHCFPGGD"
     gene            1126..2427
                     /locus_tag="VEV43_08315"
     CDS             1126..2427
                     /locus_tag="VEV43_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYP23563.1"
                     /translation="MKRDDTSGLGERIRRARIDRGLSQRDVALPLLTPSYVSLIESGK
                     RVPSPDALEHIAGVLGMDPLELRTGRSPGAEAELELQLQEARRALHSGERDEATSIAE
                     EVRGIAREDGWPRVEAKSLCVLAVVKEHQGLPEEALDLYQAAEDLSRTDPLHVRYEAV
                     AGIARCNLELGNSRLAIHILNEYLERLDRSGEPDPVARMRSHAALVHCYMHVGLRDRA
                     AHEAEQALRYAPLMDDPAELACLHMNVASSMLGQGRHGDAVAAARQAEHYFSLLDWKL
                     GASWAQMNRGIVLAEKHDLAGAREAFEEALERLETVANATADRANVLNELAQIERMSG
                     RPARAQARAAEARALIGSDGPAMILATNHREMGRALVKKDVATAEKELRTAMRMFRDA
                     GAARDAATTARDLAKVLRDRKKQREALAVLEEGLDAALQAD"
BASE COUNT          436 a          928 c          908 g          396 t
ORIGIN      
        1 cgggcgagta cgacgtgccg ctcgtcttgc aggaccgcac gttccggcgc gacgggtcgt
       61 tcaagttccg cgacagccac gacgacgtcc tcggcgaggt cttcctggtc aacggccgcc
      121 cgatgccggt cctcgaggtg gccaaccgca agtaccggtt caggatcctc aacgcgtcga
      181 actcacgcgg ctaccagctc gcgctgtcga ccggcgagcc tctgatccag gtcgcgtcgg
      241 accaggggct gctcagcgcg ccgactccct cggcgaagat cccgctgtgg acggccgagc
      301 gcgccgacgt cgtcgtcgac ttctcgaagt acccggtcgg cactcgcgtc gtcctcggcg
      361 accgtcagga cgacgccgac cccgcggggg ccgcgccgct gatggcgttc gacgtcacgc
      421 gggaggaggc cgacgacagc tcgttgccgg cgatgttccg gccgatccag aggatcctgc
      481 ccgtcccggg cacgatcgag cgcgagttcg ctctcgagtt cgacttcgac cggcagctct
      541 ggcagatcaa cggcaaggtc ttcgacccga accgcatcga cgtcaagccg aggctcggcg
      601 acaccgagat atggacgttc cgcaacctct ccaacgtgac gcacccgatg cacgtgcacc
      661 tcgtccggtt ccaggtcctc gaccgcgacg gtctcccgcc gtcgccgaac gagatgggat
      721 ggaaggacac cgtccgcgtg gggcagggcg aggtcgtgcg cgtggcgatg acgttcgagg
      781 gccacacggg ccgctacatg ttccactgcc acaacctggc ccacgaggac cactcgatga
      841 tgggtcagat gaaggtggtc gactagcccg gatcgggcgc gttgctttcg ccgttcctag
      901 tcacccccgg ggaagcagtg cttgaactgc agcacctcca cgcactcctc ggcctccgcc
      961 ggagcggcgg cgaggatcgt cgcgtacaag gtcgccatca cgaccaaacg gactctgcga
     1021 gacatcaagg acctcccttc gcgcgacaga tgtgttgact tgaagtgtgc attgtagtaa
     1081 taactggacg cgaggcgcgc ccgaggcgcc gtacgctgtc gcgctatgaa gcgggacgac
     1141 acgtcgggcc tcggcgagcg cattcgtcgc gcgcggatcg atcggggact gtcacagcgc
     1201 gatgtcgcgc tcccgctgct gacgccctct tacgtctcgc tgatcgagtc cgggaagcgc
     1261 gttccgtcgc ccgacgccct cgagcacatc gccggcgtcc tcggcatgga cccgctcgag
     1321 ctccgcacgg gacggtcgcc cggtgccgag gccgagctcg agctgcagct gcaggaggcg
     1381 cggcgcgcgc tgcactccgg cgagcgggac gaggcgacct cgatagccga agaggtccgc
     1441 ggcatagcgc gggaggacgg ctggccacgc gtcgaggcga agtcactgtg cgtgctggcg
     1501 gtcgtgaagg agcaccaagg gctgcccgag gaggcgctgg acctctacca ggccgccgag
     1561 gacctgtcgc ggacggaccc cttgcacgtt cgctacgagg ccgtcgccgg gatcgcgcgc
     1621 tgcaacctcg agctgggcaa ctcgcggctc gccatccaca tcctcaacga gtacctggag
     1681 cgcctggacc gcagcggcga gcccgacccg gtcgcacgca tgcgctctca cgccgcgctc
     1741 gtgcactgct acatgcacgt ggggctgcgg gatcgcgccg ctcacgaggc ggagcaggct
     1801 ctgcgttacg cgccgctgat ggacgacccc gccgagctcg cgtgcctgca catgaacgtc
     1861 gccagctcga tgctcggcca gggccggcac ggcgacgccg tcgcggccgc ccgccaggcc
     1921 gagcactact tctcgctgct cgactggaag ctcggcgcct cgtgggccca gatgaaccgc
     1981 gggatcgtcc tcgccgagaa gcacgacctc gcgggggcca gggaagcgtt cgaagaggcg
     2041 ctcgagcggc tcgagaccgt ggcgaacgcg accgccgacc gtgcgaacgt cctcaacgag
     2101 ctcgcgcaga tcgaacggat gagcggcagg ccggcgcgcg cccaagctcg cgccgcggag
     2161 gcccgcgctc tgatcggctc cgacgggccg gccatgatcc tcgccaccaa ccatcgcgag
     2221 atgggccgcg ctctcgtgaa gaaggacgtg gcgaccgcgg agaaggagct gcgtaccgcg
     2281 atgaggatgt tccgcgacgc gggggccgcg cgcgacgccg ccacgacggc gcgggatctc
     2341 gcaaaggtgc tgcgggatcg caagaagcag cgggaggccc tggcggtgct cgaggagggc
     2401 ctcgacgccg cgctccaggc cgactaagcc ccgggtttac taccccggcg gttttagctg
     2461 acaaattgta aagatttcag tttgatcttt ccgccctttt tctttaggtt cttcgtcgaa
     2521 ggtgcccaag ggttccggct tccccgctcg ggcttgccaa ttgctcaggc aacaccggcg
     2581 gggagacggg cctcggggcc ccctcccggc tcagcaagcc gagccgtgga ggaaccgagg
     2641 gccctcccgg cgaacctccc ggcgacag
//
LOCUS       DASQDO010000294         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1193688, whole genome shotgun sequence.
ACCESSION   DASQDO010000294 DASQDO010000000
VERSION     DASQDO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193688"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2438
                     /locus_tag="VEV43_08320"
     CDS             <1..>2438
                     /locus_tag="VEV43_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HYP23564.1"
                     /translation="SAWFALFHQRYSTNTAPAWERAQPFRMLAHNGEINTIRGNLAAM
                     KARVAAEDSDDLAPPVDARSSDSGILDGALEMLVRGGRTVEHAAAMLVPPAWENDPSL
                     GRDERGFFRFHSALMEPWDGPAGLVFSDGTTVGAALDRNGLRPLRVALCEDGFVACAS
                     EAGAIDTSGHGAVTRTRLGPGQALVVDPEHGIRLDAHVKRSLARSGEYAAAAEGALQP
                     AGRGAPVGIPAHRVLPLQVASGMTKEEVTVVLRPMATGSGEPVSSMGDDTAQPPLARF
                     RRPVFSYLKQRFAQVTNPPLDHLRESHVMSQVTLLGPRPALDATAIPPGSLREYRSPL
                     VSPEAVAELVAEGAAVVDATWDASEGAAGLRRACERAARTACHAVREGATVVVVTDAG
                     MGPERVAVPSALVAGAVHHALAAAGLRPRAGLVVDAADVRESHHVACLVTNGADLVCP
                     RLALETIADLADHARLGGDVSSGAAQELFLDALEAGLYKVMSKMGISTVASYRGAQVV
                     EAIGLGPDVIELCFPGVASVLGGSSLDDLGEDALARHEAAWSEKPALAMPGAFKHKSG
                     GEYHALNPAVIEGLQSAVTTEMEAAHALRRAVAGNPRAYEDFAELVNGRPPSEPRDLL
                     GFAPATPVPLDEVEDAASIARRFSAGAMSLGALSPEAHETIALALDMIGGMANTGEGG
                     EDPARYGDSGRSSAIKQVASARFGVTPAYLADAREVQIKMAQGSKPGEGGQLPGVKVS
                     TEIARLRHTVPGVPLISPPPHHDIYSIEDLAQLIFDLKQANPEADVSVKLVSSEGVGT
                     VAAGVVKGLADGVH"
BASE COUNT          319 a          903 c          886 g          330 t
ORIGIN      
        1 cagcgcgtgg ttcgccctgt tccaccagcg gtactcgacg aacaccgctc cggcgtggga
       61 gcgcgcccag ccgttccgga tgctggcgca caacggcgag atcaacacga tccgcggcaa
      121 cctcgccgcg atgaaggccc gggtggcggc ggaggactcc gacgatctcg cgccgccggt
      181 cgacgcgcgc agctccgact ccgggatcct cgacggcgcg ctcgagatgc tggtgcgcgg
      241 gggccggacg gtcgagcacg cggccgcgat gctcgtgccg ccggcgtggg agaacgatcc
      301 ctctctcggc cgcgacgagc gcggcttctt ccggttccac tccgcgctga tggagccgtg
      361 ggacggcccc gcgggactgg tgttctcgga cgggacgacc gtgggggccg cgctcgaccg
      421 caacggcctg cgccctcttc gagtcgcgct gtgcgaggac ggcttcgtcg cgtgtgcgtc
      481 ggaggccggc gcgatcgaca cgagcggcca cggcgcggtc acgcggacgc ggctggggcc
      541 gggccaggct ctcgtcgtcg accccgagca cggcatccgt ctcgacgccc acgtgaagcg
      601 atccctagca cgctccggcg agtacgcggc cgcggcggaa ggcgcgttgc agcccgccgg
      661 gcgtggagct ccggtgggca ttccggcgca ccgcgtcctg cccctgcagg tcgcctccgg
      721 gatgacgaag gaggaggtca cggtcgtcct gcggccgatg gccacggggt cgggcgagcc
      781 cgtgtcgtcg atgggcgacg acaccgcgca gccgccgctc gcccgcttcc ggcggccggt
      841 cttctcgtac ttgaagcagc gtttcgctca ggtgacgaac ccgccgctcg accatctgcg
      901 cgagagccac gtgatgtcgc aggtgactct gctcggcccc cgccccgctc tcgatgccac
      961 ggcgatcccc ccggggagcc tgcgcgagta ccggtccccg ctcgtgtccc ccgaggcggt
     1021 cgccgagctc gtggccgagg gggcggccgt cgtcgacgcg acgtgggacg cgtccgaggg
     1081 ggccgccggc ctgcgccgcg cctgcgaacg cgccgcccgg accgcatgcc atgccgtgcg
     1141 agaaggggcg accgtcgtcg tggtgaccga cgcggggatg gggccggagc gcgtcgcggt
     1201 tccatctgcg ctcgtcgccg gcgccgtcca ccacgcgctg gccgcggcag gtctccgccc
     1261 gcgcgcgggc ctcgtcgtcg acgccgccga cgtgcgcgag agccaccacg tcgcgtgcct
     1321 ggtcacgaac ggcgccgacc tcgtctgccc ccggctcgcg ctcgagacca tcgcagacct
     1381 cgccgaccac gcgcgcctcg gcggcgacgt ctcgtcgggg gccgcgcagg agctgttcct
     1441 ggacgcgctc gaggccggcc tctacaaggt catgtcgaag atgggcatct cgaccgtcgc
     1501 gtcgtatcgc ggcgcccaag tggtggaggc gatcggcctc gggcccgacg tgatcgagct
     1561 gtgcttcccc ggcgtcgcct cggtcctcgg tgggtcgtcc ctcgacgacc tcggagagga
     1621 cgcgctcgcg cgccacgagg ccgcatggtc ggagaagccc gcgctcgcga tgccgggcgc
     1681 gttcaagcac aagtccggcg gcgagtacca cgctctcaac cctgccgtga tcgaggggct
     1741 ccagtccgcg gtgaccaccg agatggaggc tgcccatgcc ctgcggcgtg cggtcgccgg
     1801 caacccgcgt gcctacgagg acttcgccga gctcgtcaac ggacgcccgc cgtccgagcc
     1861 ccgcgacctg ctcggcttcg cgccggcgac accggtgccg ctcgacgagg tcgaggacgc
     1921 cgcttccata gcgcggcggt tctcggccgg ggcgatgtcg ctcggcgcgc tctctccgga
     1981 ggcccacgag accatcgcgc tggcgctcga catgatcggg gggatggcga acacgggcga
     2041 aggcggcgag gaccccgccc gctacgggga cagcggacgc agctccgcga tcaagcaggt
     2101 cgcgtccgcg cgcttcgggg tcacccccgc gtacctcgcc gacgcgcggg aggtccagat
     2161 caagatggcg cagggatcga agcccggtga gggcggccag ctccccggcg tgaaggtgtc
     2221 gaccgagatc gcccggctgc ggcacaccgt gcccggcgtc ccgctgatct cgccgccgcc
     2281 ccaccacgac atctactcga tagaggacct cgcacagctg atcttcgacc tcaagcaggc
     2341 gaaccccgaa gcggacgtct cggtgaagct cgtctcatcc gaaggcgtcg ggaccgtcgc
     2401 cgcgggcgtc gtcaagggcc tcgcggacgg cgtgcacg
//
LOCUS       DASQDO010000295         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_154009, whole genome shotgun sequence.
ACCESSION   DASQDO010000295 DASQDO010000000
VERSION     DASQDO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154009"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VEV43_08325"
     CDS             complement(<1..182)
                     /locus_tag="VEV43_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HYP23565.1"
                     /translation="MTRTKVTLAGAAAAAILVAAAPAVLAHAAYKSSNPADKSTVATP
                     PTQVTAEFTEPLTEASS"
     gene            complement(232..1122)
                     /locus_tag="VEV43_08330"
     CDS             complement(232..1122)
                     /locus_tag="VEV43_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HYP23566.1"
                     /translation="MKKTVTVAAVAAATALLAGAALAHTSIAESDPADNSTVEALPAE
                     VSIRFGNPDVPVPQPAQLTEATLLLLDPCGARVDNEDAAWDQTTSTISASTKASETAG
                     RYEMHWSGTSTDGDTQAGYVDFVVSGGEQCALVQRTDAADDIDLGFNPTKVTSKPTAA
                     GANVTVALAAAPACKAFRVEGQRLELAMDTNWDESVDYTGAFTCRAKKVRRNGVVKNV
                     AVYGLEVTKASDETPSLKFKVRKTGAKALTAAVPSTILEDQEAGSLDLYVSSTTESDE
                     CGEDKSCADRAPDLGWVRSL"
     gene            complement(1155..1709)
                     /locus_tag="VEV43_08335"
     CDS             complement(1155..1709)
                     /locus_tag="VEV43_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23567.1"
                     /translation="MTGKNLARALTALALAATALLPTGAHAAPMKVLGTDAALDGPPG
                     ADLVSLAAAKHGSDFHIQIGLAQALPQQGSYPGAGIEWAFEVRGRTFLAEAHPEPGAM
                     RYTLFEIQGDSLRQVEILEGSFDTESGLIDMYVPLASIGARKGTKISGAGDGSTPDVD
                     IHQHAGPASRELDALTTTKDFVVR"
     gene            complement(1774..2367)
                     /locus_tag="VEV43_08340"
     CDS             complement(1774..2367)
                     /locus_tag="VEV43_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23568.1"
                     /translation="MSTTVPAVKRAWLGGLACAGVAAAHWIAYLIAPPDGHSHAHGMA
                     ATGHKLWPYFVAVAVAALSGFVSDRFGEEQGASGAALWRQAAGRLVPAQVLAFACLEL
                     AERALFADPGHAGTLLTEPVLWVGIALQVVCALAGAGLLVLFARTVELIQELVGRRLA
                     TATAPRVLPHRLFAHLVVRPLVMATGGPTFRGPPVLL"
     gene            complement(2408..2908)
                     /locus_tag="VEV43_08345"
     CDS             complement(2408..2908)
                     /locus_tag="VEV43_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23569.1"
                     /translation="MPVLAHTATSSGPNTEILLLGVGMIVLALVFFFQKTASRQASLV
                     LAVLGAVAVTGAFTLSGGGGSGDDHHDVSISIAEPAEGATVEAGTVAVEVELTGAELA
                     GESSSEDAGHLHVVVDGEVVDMPSSLDLEVELEAGEHEIEVEFVDADHAQLDPPVRDS
                     VTVVAE"
     gene            complement(2924..>3333)
                     /locus_tag="VEV43_08350"
     CDS             complement(2924..>3333)
                     /locus_tag="VEV43_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23570.1"
                     /translation="ERARGARSLQEAARGFGWSLVPIGVAYVIAHNAPLLLTGVPELL
                     RVVSDPFEYGWNLFGTAELWSGFSPSPQLVWYIEIAVIVGGHVLGVLAAHRTAVRVAG
                     DHASAVRSQYALTALMSVYTIATLWLLAQPLVR"
BASE COUNT          474 a         1157 c         1213 g          489 t
ORIGIN      
        1 gaggaggcct ccgtcagcgg ctcggtgaac tcggccgtga cctgggtggg gggggtggcg
       61 accgtcgact tgtcggcggg gttcgagctc ttgtacgccg cgtgcgcgag gacggccggg
      121 gccgccgcga cgaggatcgc cgcggcggcc gctccggcca gcgtcacctt cgttcgagtc
      181 atcgtcatcg tccttcccac agtacgtgtg aagacactct tgggcacggg ctcacagcga
      241 acggacccag ccgaggtcgg gggcccgatc ggcgcaggac ttgtcctcgc cgcactcgtc
      301 cgactcggtc gtcgacgaca cgtagaggtc gagcgagccc gcctcctggt cctcgaggat
      361 cgtcgagggc accgcggcgg tgagcgcctt ggcccccgtc ttgcgcacct tgaacttgag
      421 cgacggagtc tcgtcgctcg ccttggtcac ctcgaggccg tagaccgcga cattcttgac
      481 gacgccgttg cggcgcacct tcttcgcgcg gcacgtgaac gcacccgtgt agtcgacgct
      541 ttcgtcccag ttcgtgtcca tggcgagctc gagcctctgg ccctcgaccc tgaacgcctt
      601 gcacgcggga gcggcggcga gcgcgacggt cacgttcgcg ccggcggccg tcggcttcga
      661 cgtgaccttc gtcgggttga agcctaggtc gatgtcgtcg gcggcgtcgg tccgctggac
      721 gagcgcgcac tgctcgccgc ccgagacgac gaagtcgacg tagccggcct gcgtgtcacc
      781 gtcggtagac gtgcccgacc agtgcatctc gtagcgtccg gccgtctccg atgccttggt
      841 cgacgcgctg atcgtgctcg tcgtctggtc ccacgcggcg tcctcgttgt cgacgcgcgc
      901 gccgcagggg tcgagcaaga gcagcgtcgc ttcggtgagc tgagccggct ggggcaccgg
      961 gacgtcgggg ttgccgaagc ggatcgagac ctcggccggg agagcttcga cggtcgagtt
     1021 gtccgcgggg tccgactccg cgatcgaggt gtgcgcgagc gccgctccgg cgagcagcgc
     1081 cgtcgccgcg gcaaccgccg cgaccgtgac ggtcttcttc atcgtggttc ctcctgatga
     1141 gtcgtggggg gggttcagcg gacgacgaag tccttcgtcg tcgtcaacgc gtcgagctcg
     1201 cgcgaggccg gccccgcgtg ctggtggatg tcgacgtcgg gcgtcgaacc gtcaccggca
     1261 ccggagatct tcgtgccctt gcgcgcgccg atcgacgcga gcgggacgta catgtcgatc
     1321 agacccgact cggtgtcgaa ggaaccttcg aggatctcga cctgacgcag cgagtcgccc
     1381 tggatctcga acagcgtgta gcgcatcgcg cccggctcgg ggtgtgcctc cgcgaggaac
     1441 gtgcggccgc gcacctcgaa tgcccactcg atgcccgcgc ccgggtagct cccctgctgg
     1501 ggcagcgcct gcgcgaggcc gatctggatg tggaagtcgc ttccgtgctt cgccgcggcg
     1561 agcgacacga ggtcggcccc gggcggaccg tcgagggcgg cgtcggtgcc gaggaccttc
     1621 atcggcgccg cgtgtgcccc cgtgggcagc aacgccgtcg ctgcgagtgc cagcgcggtc
     1681 agcgcgcggg cgaggttctt gccggtcatt tcttttcctc cgagtgatgg gttcgatcgg
     1741 gcggcgccgg acggcgccgc gcgcggaacg gactcagagg agtacgggtg gcccccggaa
     1801 ggtcggcccg ccggtagcca tgacgagcgg acggacgacg aggtgtgcga agagacggtg
     1861 cggcaggacc cgcggcgcag tcgccgtggc gaggcgccgc ccgacgagct cctggatgag
     1921 ctcgacggtc cgggcgaaca gcacgagcag cccggccccc gcaagggcgc agacgacctg
     1981 gagcgcgatg ccgacccaga ggacgggctc ggtcagcagc gtcccggcgt gcccggggtc
     2041 ggcgaacagt gcccgctcgg cgagctcgag gcatgcgaac gcgaggacct gggccgggac
     2101 gagccgcccc gccgcctggc gccacagagc cgcgcccgag gcgccctgct cctcgccgaa
     2161 ccggtcggag acgaacccgc tgagggccgc gacggccacc gcgacgaagt acggccagag
     2221 cttgtggccg gtggcggcca tcccgtgggc gtggctgtgt ccgtcgggag gggcgatcag
     2281 gtatgcgatc cagtgcgcgg ctgcgacgcc ggcgcaggca aggccaccca gccaggctcg
     2341 tttgaccgca ggcacggtgg tgctcatgcc gctctggtac gccgtcgggg gcccaaaacc
     2401 tgcctcctca ctcggccaca acggtcacgg agtccctgac cggggggtcg agctgggcgt
     2461 ggtcggcgtc gacgaactcg acctcgatct cgtgctcgcc ggcctcgagc tcgacctcga
     2521 ggtccaggct cgagggcatg tcgacgacct cgccgtccac gacgacgtgg aggtggccgg
     2581 cgtcctcgct cgagctctcg ccggcgagct ccgcccccgt cagctcgacc tcgaccgcaa
     2641 cggtcccggc ctcgaccgtc gccccctcgg ccggctcggc tatggagatc gagacgtcgt
     2701 ggtggtcgtc gcccgatccc ccgccgccgc tgagagtgaa cgcccccgtc acggcgacgg
     2761 cgcccagaac ggcgaggacg aggctcgcct ggcgggaggc ggtcttctgg aagaagaaga
     2821 cgagggccag cacgatcatg cccaccccga gcaacaggat ctcggtgttc gggccgctcg
     2881 acgtggcggt gtgggctagc acgggcacgg cttcctccct cctctaacga acgagcggct
     2941 gcgcgagcag ccacagggtc gcgatcgtgt agacgctcat cagcgcggtg agcgcgtact
     3001 ggctccgcac ggcggacgcg tggtcgcccg cgacgcgcac cgccgtccgg tgggccgcga
     3061 ggacgccgag cacgtgcccc ccgacgatca ccgcgatctc gatgtaccag acgagctggg
     3121 gcgaaggcga gaaccccgac cacagctcgg cggtcccgaa gaggttccac ccgtactcga
     3181 acgggtccga caccacgcgc aatagctcgg ggacccccgt gaggagcaac ggtgcgttgt
     3241 gcgcgatcac gtaggcgacg ccgatcggga cgagcgacca gccgaagccc cgcgcggcct
     3301 cctgaagtga gcgcgcgccg cgggcgcgct ccc
//
LOCUS       DASQDO010000296         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_643277, whole genome shotgun sequence.
ACCESSION   DASQDO010000296 DASQDO010000000
VERSION     DASQDO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643277"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1237)
                     /locus_tag="VEV43_08355"
     CDS             complement(176..1237)
                     /locus_tag="VEV43_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HYP23571.1"
                     /translation="MLVVEDNPADVRLLRVALATDRRSRYEVDSVDSVADALRLIDAY
                     DYDAALLDLTLPDSTGLASFDRVHAAAPDLPVVILTQTEDDSLAASAVAAGAQDYLVK
                     GQTDAVLLGRTVRHAVERQRLVNQLRQMDRARREFIAHAAHELRTPLTTIAGMSALIE
                     ERRGDLGEERFERMLQAVLSQSRRAADLARALLDLSQVESGDLALDVKPTDVGSVVRD
                     AVRASPAGDAVDVTVEEGIDALVDRARLEQILVNLVSNAGRYGRRVWVTARQREDRVL
                     VAVEDDGPGVPDELVPHLFEPFVRGPAARGEGTGLGLTITRRLVEALKGTIVYEPREP
                     TGAKFAVWLPAAERAATER"
     gene            complement(1263..2786)
                     /locus_tag="VEV43_08360"
     CDS             complement(1263..2786)
                     /locus_tag="VEV43_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYP23572.1"
                     /translation="MIEGLPDEAAFFAAIVESTDDAVLSKTLDGTITSWNAAAERMYG
                     YTRAEAVGNNVRMLLPPGRPGEIDQILDRIRSGERIEHFDTYRRRKDGRILDVSLTIS
                     PVRDADGVVVGASTIARDVTERREAQEALERYTREIEQSNRDLERFAYVVSHDLAEPL
                     RMISSFVQLLGEEFGDRLDDRGREYVDFVLEGSARMRALIDDLLAYSRLGSEAVAVTA
                     VDLGVVAGRVVDVLAVAIHEAGAEVTVDDLPEVAADAIKIEQLLRNLVANALKFRRED
                     EAPRVHIDAGRVAGGCRICVSDNGIGIEPRHSERVFEMFQRLHTRDEFPGTGVGLTIC
                     KRIVDLHEGRIWFEPNEPHGTRFCFTIPDRDRVEQEAVHVLLVEDNPGDVLLMRQGLA
                     TWNHPVEVHVVSNGEEALDFLRREGPHAGAAAPDLVLLDVNLPRLKGDEVLARMRADP
                     SLERIPVAVMSSSSAEARIAAAYDSTTTTFVLKPMDVPEYRAAVRDVERFWTIAARR"
     gene            complement(2791..>3339)
                     /locus_tag="VEV43_08365"
     CDS             complement(2791..>3339)
                     /locus_tag="VEV43_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HYP23573.1"
                     /translation="RASVGCRTVPNLTRDELTLAVAVLAGVLLVAVLLLLIFALRLKK
                     IRREYVLLRGDGDEKDVIAAVARAMRRVEAIDKRVDGVVSSLQEHAATGRYALQRFGL
                     VRYDAFEEMGGRLSFSAALLDDHGDGLLITSINGRTETRTYSKEIKQLTSEHNLSEEE
                     REAIAAAMAGYGRGESRAELSR"
BASE COUNT          495 a         1203 c         1127 g          514 t
ORIGIN      
        1 cgtcggtgaa ggacggcccg ttcttgaagt ggacgttggc ggccacggct accgacgtga
       61 cggcgacggc ggccaccacc cccagcaaag cacccctcag aattcgcatg tcgctcctcg
      121 ctcaatggat ttcctggcaa attctgcctg gaagaaccac ccttggcaag agggttcagc
      181 gctcggtcgc ggcccgctcg gccgcgggga gccacaccgc gaacttggca ccggtcggct
      241 ccctcggctc gtagacgatc gtccccttca gagcctccac gagacggcgc gtgatcgtga
      301 ggcccagccc ggtgccctcg ccgcgcgccg ccgggccgcg cacgaacggc tcgaagagat
      361 gcggcacgag ctcgtcgggg acgcccggcc cgtcgtcctc gaccgcgacg agcacgcggt
      421 cctcccgctg cctcgccgtc acccacaccc gccggccgta ccgccccgcg ttcgagacga
      481 ggttcacgag gatctgctcg agccgggcgc ggtccacgag cgcgtcgatc ccctcctcga
      541 ccgtgacgtc caccgcgtca ccggccggcg acgcacgtac cgcgtcgcgc accaccgaac
      601 cgacgtcggt cggcttcacg tcgagggcga ggtcgcccga ctcgacctgc gagagatcga
      661 gcagcgccct cgcgaggtcc gcggcgcggc gcgattggga gagcacggcc tggagcatcc
      721 tctcgaaccg ctcttcgccg aggtcaccgc gccgttcctc gatcaaggcc gacatgcccg
      781 cgatcgtcgt aagaggggtc cggagctcgt gtgccgcatg cgcgatgaac tcgcggcgcg
      841 cgcggtccat ctggcgcagc tggttcacga ggcgctggcg ctcgacggcg tgcctcaccg
      901 tgcgtccgag gaggacggcg tcggtctgtc ctttcaccag gtagtcctgc gctcccgcgg
      961 ctacggccga cgccgcgagt gagtcgtcct cggtctgcgt gaggatcacg accggcagat
     1021 cgggggcggc cgcgtgcacg cggtcgaacg acgcgaggcc ggtcgagtcc ggcagcgtca
     1081 ggtccagcag cgcggcgtcg tagtcgtaag cgtcgatcag acgaagcgcg tccgcgaccg
     1141 aatcaacgga gtccacctcg taacgcgacc gccggtcggt cgcgagcgcg acccgcagga
     1201 gacgcacgtc ggcggggttg tcctcgacga ccagcactct caacgcgacg tccagctcgc
     1261 cctcacctcc tcgcggcgat cgtccagaac cgttcgacgt cacgcaccgc ggcgcggtac
     1321 tcgggcacgt ccatcggttt gaggacgaag gtcgtagtcg tcgaatcgta cgccgcggct
     1381 atccgggctt cggccgagga gctgctcatc accgcgacgg ggatccgctc gagcgaggga
     1441 tcggcccgca tgcgcgcgag gacctcgtca cccttcagcc gggggaggtt cacgtcgagc
     1501 agcacgaggt cgggggccgc ggcccccgcg tgcggccctt cacgtcgcag gaagtcgagt
     1561 gcctcctcgc cgttcgagac gacgtggacc tcgacggggt ggttccacgt cgcgagcccc
     1621 tgccgcatca gcaggacgtc gccggggttg tcctcgacca ggaggacgtg gactgcttcc
     1681 tgctccacgc ggtcccggtc ggggatcgtg aagcagaagc gagtcccgtg cggctcgttc
     1741 ggttcgaacc agatacgtcc ttcgtggagg tcgacgatcc gcttgcatat ggtcaggccg
     1801 acaccggtcc cggggaactc gtcgcgcgtg tggagccgct ggaacatctc gaacacgcgc
     1861 tccgagtgcc ggggttcgat cccgatgccg ttgtccgaca cgcagatccg gcaaccgcct
     1921 gcgacgcgcc cggcgtcgat gtgcacgcgc ggagcctcgt cctcgcgccg gaacttcaga
     1981 gcgttcgcga cgaggttgcg cagcagctgc tcgatcttga tcgcgtccgc cgctacctcg
     2041 ggcaggtcgt ccacggtgac ctcggccccc gcctcgtgga tcgccaccgc gaggacgtcg
     2101 acgacgcggc ccgcgacgac accgaggtcg acggccgtga ccgctaccgc ctccgacccc
     2161 agacgcgagt aggcgagcag gtcgtcgatc agcgcgcgca tccgcgccga cccctcgagg
     2221 acgaagtcga cgtactcgcg tcccctgtcg tccaggcggt cgccgaactc ctcgccgagg
     2281 agctggacga agctggagat catgcgcagc ggctccgcga ggtcgtgcga gacgacgtaa
     2341 gcgaaccgtt cgaggtcgcg gttcgactgc tcgatctcgc gcgtgtagcg ctcgagcgcc
     2401 tcctgcgctt cgcggcgctc ggtcacgtct cgggcgatcg tgctcgcgcc gacgacgacc
     2461 ccgtccgcgt ccctgaccgg cgagatcgtc agcgacacgt cgaggatccg gccgtccttc
     2521 cgcctgcggt acgtgtcgaa gtgctcgatc ctctcgccgc tgcggatccg gtcgaggatc
     2581 tggtcgatct cgccgggccg gcccggcggc aggagcatcc ggacgttgtt gcctacggcc
     2641 tcggcgcgcg tgtacccgta catccgctcg gccgctgcgt tccaggaggt gatcgtgccg
     2701 tcgagcgtct tcgacaggac ggcgtcgtcg gtggactcga cgatggcagc gaagaaggcc
     2761 gcctcgtccg gcagcccctc gatcaccggg tcagcgggag agctccgctc gcgactcgcc
     2821 acgaccgtag cccgccatcg ccgccgcgat cgcctcgcgc tcctcctccg agaggttgtg
     2881 ctccgaggtg agctgcttta tctccttcga gtacgtgcgc gtctcggtcc gcccgttgat
     2941 cgacgtgatc aggagaccgt cgccgtggtc gtcgaggagc gcggcgctga acgagagccg
     3001 gccccccatc tcctcgaacg cgtcgtagcg gacgagcccg aagcgctgta gcgcgtagcg
     3061 gccggtcgcg gcgtgctcct gcagcgacga gacgacgccg tccacgcgct tgtcgatcgc
     3121 ctctacgcgc cgcatcgcac gagcgaccgc cgcgatcacg tccttctcgt cgccgtcgcc
     3181 gcgcagcagc acgtactcgc gccggatctt cttcaggcgc agggcgaaga tcaggaggag
     3241 gagcaccgcc acgagaagga ccccggcgag gacggccacc gccagcgtga gctcgtctct
     3301 cgtgaggttc ggcacggtgc ggcatcctac actggcgcg
//
LOCUS       DASQDO010000297         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_582183, whole genome shotgun sequence.
ACCESSION   DASQDO010000297 DASQDO010000000
VERSION     DASQDO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582183"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..577
                     /locus_tag="VEV43_08370"
     CDS             95..577
                     /locus_tag="VEV43_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23574.1"
                     /translation="MFDELRRMALFGSGIAELTRYRAEQMVKDLVKAGDIRREQAQGA
                     VRDMLEMARANRKELLGLVRAEISDQIANLGVANGRDVERLERRVARLEDELRKLKND
                     AREAAKPPAGKKTTAKKPTAKKTTAKKTTARKTSATSKKTPAGRSPGAGKPAETGAGE
                     "
     gene            588..758
                     /locus_tag="VEV43_08375"
     CDS             588..758
                     /locus_tag="VEV43_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23575.1"
                     /translation="MTTPLPDEDVVAGATSRLDAAQDASDQERLRVLDELYAGLEAEL
                     ERDLEQAGPAGR"
     gene            730..1518
                     /locus_tag="VEV43_08380"
     CDS             730..1518
                     /locus_tag="VEV43_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HYP23576.1"
                     /translation="MSKPARLDAELVRRGLAGSRAEAKHAIEQGKVAVRGVPASRATT
                     LVSPADPITLTAPAARFVSRGGDKLDGALARLDVTVAGRRWLDAGASTGGFTDRLLQG
                     GAAAVVAADVGYGQLDWRLRNDARVVVIERLNLRELKPEHLPWRPEGVVADLSFISLR
                     LVLPALAAAAETDADFVVMVKPQFEVGRDVVGKGGVVRDPALWVDSMKGVAETARELG
                     LGLAGAAPSEVPGPAGNREFFLHLRAGGFGGDEMLRAVADEVAP"
     gene            1515..2297
                     /locus_tag="VEV43_08385"
     CDS             1515..2297
                     /locus_tag="VEV43_08385"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYP23577.1"
                     /translation="MTIERVAIVLNDGKPAAKERARDLAKLLHEGGIAVALEEPDLVV
                     SLGGDGTMLRAAQHAHAADVPLLGVNLGTLGYLTEVDASHEVEAVRRVLAGDFETQQR
                     MMLSCEAAGRTFVALNEVLVERASRRRLVRLAVRVGGENLGDFNADGIIVATPTGSTA
                     YALSAGGPIVSPRAECLVVVPVSPHMVFARPFVLAADEVVEITVRDSGQDASLSLDGH
                     LGSDLGPGSTAVVRRHERPLKLVRLGGPRFIERLRTKMNLPG"
     gene            2331..3128
                     /locus_tag="VEV43_08390"
     CDS             2331..3128
                     /locus_tag="VEV43_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23578.1"
                     /translation="MKVALAAALCLLLGAAPVAAKPPAGEMVLRGDSSASVELRLAQP
                     ADVFCCRMVFEEVDGRSYFRIAGFEVETEGTFAGFAIERPSDGRILRGAVRLPAMDLD
                     DGIVPGFASFGRTRRLPAGRYRIHLLTDGPSTVRFTVPGLQRDVHLRPEGASDVTASL
                     VELTPEDVQERVPVEVPGDALVVLVSKTEGDFTQGHYLSHCLAAPGAPCTSSEDYEVW
                     TSPGSGGGGGTRVDSLYDPPAGSLEAVFTSRSLGVPAGTYGFVLVLG"
     gene            3171..>3756
                     /locus_tag="VEV43_08395"
     CDS             3171..>3756
                     /locus_tag="VEV43_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP23579.1"
                     /translation="MNPMLHDLVVEGLGVIGRAEIALGPGSTALTGETGAGKTLLVAA
                     LGLLTGGRADRALVRAGAAEARVEGRWVLSAEHPAVEALADAGLIEEAAGEVEVVLTR
                     TVSADGRGKARVNTRVVPIATLADVGRHLVEIAGQHEHQRLSQPAWQRGLLDAFAGDD
                     AVALAETVAAEVRAAGAAARRLAGASEDERTRERT"
BASE COUNT          533 a         1331 c         1397 g          495 t
ORIGIN      
        1 cgagatcggg aagagcgaca gcaccgacgg agtatagatt cgccgccctg caacaccgca
       61 gacgaagacg aacgaccgga gggatcgagg gacgatgttc gacgagttgc gccgcatggc
      121 tctgttcggc tccggcatcg ccgagctcac ccgctaccgg gccgagcaga tggtcaagga
      181 cctcgtgaag gccggggaca tcagacgaga gcaggcccag ggcgcggtgc gcgacatgct
      241 cgagatggcg cgtgccaacc gcaaggagct cctcggcttg gtccgcgccg agatctccga
      301 ccagatcgcg aacctcggcg tcgcgaacgg ccgcgacgtc gagcgtctcg agcgccgggt
      361 cgcccggctc gaggacgagc tacgcaagct gaagaacgac gcgcgggaag ccgccaagcc
      421 gccggcgggc aagaagacga ccgcgaagaa gcccaccgcg aagaagacga ccgcgaagaa
      481 gacgaccgcg cgcaagacct cggcgacctc gaagaagacg ccggccggac ggtcccccgg
      541 cgcgggcaaa cccgccgaga cgggcgcggg ggagtgataa tcgagccatg acgacgccgc
      601 ttcccgacga agacgtagtc gccggcgcga cgagccgcct cgacgccgcg caggacgcta
      661 gcgaccagga gcgcctgcgc gtcctcgacg agctctacgc cggcctcgag gccgagctcg
      721 aaagggatct tgagcaagcc ggcccggctg gacgctgagc tggttcgacg cgggctcgct
      781 ggctcgaggg ccgaggccaa gcacgcgata gagcagggga aggtcgccgt acgcggagtc
      841 cccgcgtccc gcgcgacgac gctcgtctct cccgccgacc cgataacgct gacggccccc
      901 gcggcccgct tcgtctcgcg cggtggtgac aagctcgacg gtgccctcgc ccgcctcgac
      961 gtgacggtgg ccgggcgacg ctggctcgac gcgggagcgt ccacgggcgg gttcacagac
     1021 cggctcctcc agggcggagc ggcggcggtc gtcgcggccg acgtcgggta cgggcagctc
     1081 gactggaggc tccgcaacga cgcgcgcgtc gtggtgatcg aacgcctcaa cctccgcgag
     1141 ctgaagcccg agcacctccc gtggcggccc gagggcgtgg tcgcggacct ctcgttcatc
     1201 tcgctgcgtc tggtgctgcc cgcgctcgcc gcggccgccg agacggacgc ggacttcgtc
     1261 gtgatggtca agccgcagtt cgaggtcggg cgcgacgtgg tcggcaaggg cggcgtcgta
     1321 cgcgacccgg ccctgtgggt cgactcgatg aagggggttg ccgagaccgc ccgcgagctc
     1381 ggcctcggcc tcgccggcgc ggccccctcc gaggtgccgg gcccggccgg caaccgcgag
     1441 ttcttcctgc acctccgcgc cggaggcttc ggcggcgacg agatgctgcg cgcggtcgcg
     1501 gacgaggtcg cgccgtgacg atcgagcgcg tcgccatcgt cctcaacgac gggaagcccg
     1561 cggcgaagga gcgggcgcgc gacctagcga agctgctgca cgagggaggg atcgcggtcg
     1621 cgctggaaga gcccgacctc gtcgtctcgc tcgggggtga cgggacgatg ttgcgcgccg
     1681 ctcagcacgc gcacgccgca gacgtccctc tgctcggcgt caacctcggc acgctcggct
     1741 atctgaccga ggtcgacgcg tcgcacgagg tcgaggcggt ccgccgcgtg ctcgccggag
     1801 acttcgagac gcagcagcgg atgatgttgt cgtgcgaggc cgcgggccgg acgttcgtcg
     1861 cgctcaacga ggtgctcgtc gagcgcgcgt cgcgccggcg gctcgtgcgc ctggcggtga
     1921 gggtcggcgg cgagaacctc ggcgacttca acgccgacgg gatcatcgtc gcgactccca
     1981 cgggctcgac cgcgtacgcg ctgagcgcgg gaggcccgat cgtgtcgccc cgcgccgaat
     2041 gcctcgtggt cgtgccggtc agcccgcaca tggtcttcgc gcggccgttc gtgctcgccg
     2101 cggacgaggt cgtggagata acggtgcgcg actcgggcca ggacgcgtcg ctgtcgctgg
     2161 acgggcacct cgggtctgac ctcggccccg gctcgaccgc ggtcgtccgg cggcacgaac
     2221 ggccgctcaa gctggtgcgt ctgggggggc cgcgcttcat cgagcgcctg cgcacgaaga
     2281 tgaacctccc gggctgagga agccgccggc ccgcggcgaa cccatctgcc atgaaggtcg
     2341 cgctggcggc cgcgctgtgc ctgctgctcg gcgcggcccc cgtagccgca aagccgcccg
     2401 cgggcgagat ggtcctcagg ggtgattcca gcgcgtcggt cgagctgcgt ctcgcacagc
     2461 ccgccgacgt cttctgctgc cggatggtct tcgaggaggt cgacggccgc tcctacttcc
     2521 gcatcgcggg gttcgaggtc gagaccgaag gcacgttcgc cggcttcgcg atcgagcggc
     2581 cgagcgacgg ccgcatcctc cggggagccg tccgcctgcc ggcgatggac ctcgacgacg
     2641 ggatcgtccc gggcttcgcg tcgttcggac ggacacggcg gctgcccgcc ggccggtacc
     2701 ggatccacct gctgaccgac ggaccttcga cggtccgttt caccgtcccg ggcctgcagc
     2761 gggacgtgca cctccggccg gagggagcct cggacgtcac tgcgtccctg gtcgagctca
     2821 cgcccgagga cgtccaggag cgggtgccgg tcgaggtgcc cggggacgcg ctggtcgtgc
     2881 tggtcagcaa gaccgaaggc gacttcaccc aggggcacta cctcagccac tgcctcgcgg
     2941 cccccggcgc gccctgcacg agctccgagg actacgaggt ctggacctcg ccggggtccg
     3001 gaggtggagg cgggaccagg gtcgactccc tgtacgaccc gccggcaggc tcgttggaag
     3061 cggtcttcac gtcgcgcagc ctcggcgtcc ccgccggcac ctacgggttc gtcctcgtcc
     3121 tcggctagcc cggcccccgg ctccggtttt cgtcggagcc tgaacttagg atgaacccga
     3181 tgcttcacga cctcgtggtc gagggcctcg gcgtcatcgg gcgggccgag atcgccctcg
     3241 ggcccggctc caccgccctc acgggcgaga cgggggccgg gaagaccctc ctcgtcgccg
     3301 ctctcggcct gctcaccggc gggcgggccg accgcgctct ggtgcgcgcc ggtgccgccg
     3361 aggcgcgcgt cgagggccgg tgggtcctgt cggccgagca tccggcggtc gaggccctgg
     3421 ccgatgcggg acttatcgaa gaagcggccg gcgaggtcga ggtcgtgctc acgcggaccg
     3481 tctctgcgga cgggcggggc aaggcgcgcg tcaacacccg cgtcgtcccg atcgcgactc
     3541 tcgccgacgt cggtcggcac ctcgtcgaga tcgcgggcca gcacgagcac cagcggctgt
     3601 cgcagccggc gtggcagcgc ggtctcctcg acgcgttcgc cggtgacgac gcggtcgcgc
     3661 tggccgagac cgtggcggcc gaggtccggg cggcgggggc cgcggcacgg cgtctcgccg
     3721 gcgcgtccga ggacgagcgg acgcgcgagc gaactg
//
LOCUS       DASQDO010000298         8005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_276486, whole genome shotgun sequence.
ACCESSION   DASQDO010000298 DASQDO010000000
VERSION     DASQDO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276486"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /locus_tag="VEV43_08400"
     CDS             complement(<1..1981)
                     /locus_tag="VEV43_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23580.1"
                     /translation="MTATRSRTPWYSRALRIVLTLMVGGTVFAPFAVAQHGHGTSAPP
                     LCGADRGGWESIDVPFPEGSQAIKSWSISKTTTLMVATNGLVVMRSVDRGCSWNVAWR
                     LDAVPANGTTATSTESVIEQVHVSESPLAPNKVLVTVDQGAPVSRPRVFVSNDGAQTF
                     EPADRGLEASVGRPEEIATAESNPGRAFLLTDTSGIQRSLLPDGLPPELQGAKGVAQV
                     LYESVDGGKSWTPMTPVYSGDPWDGIEVDPRDADVVWLHGDKGIERASTVPPPAPSDP
                     PRPAPISDVDVWHQAGQPARVIATTTDGRVYRSADGGSVFADATLPGAAQSLVIMSPI
                     QEVYSTSTGVWLLTSGQPAFDLTPTDHRPILDLHVTDGAGTELWGRSETTIERKVFAG
                     PTRQTFDLCKTAAKNGIDLPGCPHTEQDFEVPCVDLDDAGEPALQPANVDLTLKPGQS
                     RTVHFDFDLPAKGQQVDVFFLIDVSGSMQDAIDGTARAMNEIVTHLTNRGHDVWFGIG
                     EYRSYDQPPAYRRVLDVTNNCGAVEEALESLIAFGGGEETQLEALYQVARGESRPGVG
                     AVVPAGQAATFRPDARRIIIHTTDEEITEGPPNPSYDEVEEALDSAEIDQVGIAIEDG
                     FTTSGNGPLENDAPPSEGLKIVARRTETLAPEGGVD"
     gene            2113..3036
                     /locus_tag="VEV43_08405"
     CDS             2113..3036
                     /locus_tag="VEV43_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYP23581.1"
                     /translation="MSIAAVLFAAGRGHRLKPLTDVVAKPALPLLDVPLAAFGLRRLF
                     EAAPAVVVNVRHLPESVTAAVAPYVPGECRLDALFEAPEAYGTAGTLVALRERLGSPV
                     LTWNADVLTDLSAVSLLAAHRASGAPATVAVAEVSSGADFVLEGGRAAGFVDRRSRDS
                     AAGGLFLGVGVFERPVLSSLPETRPLGLGETLLASLSRSGDLAVFRHDGYWRDVGTPA
                     AYLAASLELLDGSGPEPPDGSWPGEIVAGSYLGRGAVAAGRVERSVLLGGSRVEAGAR
                     VERSLVWRDEVVPAGTVLQDGVWFGGRALPL"
     gene            3065..3505
                     /locus_tag="VEV43_08410"
     CDS             3065..3505
                     /locus_tag="VEV43_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23582.1"
                     /translation="MKVRAALVEALVRLSLLVVAGALLRWATVTLRDWLEDFNATLER
                     TWGGWFASLALVAAAGFLVGLAALAGRPRGYRILIPLVIALPPLVLLGHYYLVIENAV
                     SNGDDLPWILGHYFFYMEPAAQYVLALVVGFGVASGLQPAARQS"
     gene            complement(3496..4056)
                     /locus_tag="VEV43_08415"
     CDS             complement(3496..4056)
                     /locus_tag="VEV43_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23583.1"
                     /translation="MGRVLLCAVFLFGLAACDGGSDERPPGSSSGLVRAQARLAVLED
                     RLAEARARVDELELEAAVQPNRYVDLWRGGPTMRGPLLALPRLGLLRWTCDRDYGFRV
                     VFEGYGASVYADYHVPGFNSARMIHPGQSLGATVTPGESVVWTITHRHKPGFIRARID
                     VTTARSKHGNCLLPEVRLRETGRLYD"
     gene            complement(4106..5395)
                     /locus_tag="VEV43_08420"
     CDS             complement(4106..5395)
                     /locus_tag="VEV43_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23584.1"
                     /translation="MTRQVTRLTASTVVLALTLLSAPAWGVHARQAPTSPQCISADFS
                     PEFVTDRKGFCVAFDTSDGDASTFRFFLTSDGGRTWARRAATGFAPGTDEIMGSVIAS
                     PGYAKDRTVFVQTNRGVFSTVDDGASFALVDNFAATGPSGPNLSAFVDDAVGLPGSPE
                     GERPVLALAAGDASVKIDPPHRIPVTGAPLDERRFSFPGYRDGERLAYTMTVEVDNTQ
                     GITSRSRNVLFRCNEQLVCAEPLFAFPWGYQFEPRGGLWFAPDFSESERIYATTSKLM
                     RGLSTLKAFRSKDGGATFRPWASVNRMLRPIRRLDGSTPTIGLGLHPTRPRTIYMRLG
                     YRLPADAEDRYAPVERFFVSRDRGRTWDLLSFARDAEQRGPRGTIPWDGTWGYPVNNF
                     VSVTGDGTIFVMARDLGGHSGLYCSTDGGRTWAATCR"
     gene            complement(5500..6075)
                     /locus_tag="VEV43_08425"
     CDS             complement(5500..6075)
                     /locus_tag="VEV43_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23585.1"
                     /translation="MNVMSKLVVTSAAAVTLLFGTSASPVPASEDVRAPISKGSWLGS
                     SWADCSDETGTTGDYWCSFTGEAPADFPPGSYCAETPAAVAVSLECGASLSATTSGKL
                     KKAACFNTPLPNQLTTTNGTVTVFSSATQQSYAVPVKVWVSRGVGKFAGVGNFLLTVV
                     EVSGNFTTTCSILQSRGTFEGEFSLVTAART"
     gene            complement(6098..7216)
                     /locus_tag="VEV43_08430"
     CDS             complement(6098..7216)
                     /locus_tag="VEV43_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23586.1"
                     /translation="MHFSGDFGFCAYVSVPEQGPPHLGLYRTDDAGTSWGSRAAAGVQ
                     LVDGDVLGAVVVSPWFEEDETVYVQVGRGLFASEDGGETFSLADPLAGSGLSWRNLSP
                     FVEELGGNPSLAFAFANDDVSALVRPPLHQIVPGSPFSDRFFLVSPEARSAEGARAVA
                     VEVDSSDPQRPRARTLVFGCDANLVCQQQLHAFPWGPIPIRAWRLPSAGGDDVVVLVL
                     SEPAKDGPAVWWSHDGGATFAEWTAVDRIVRRVPVGAQVEVGVTGTVGERLYLRVSRS
                     LGLDPEDRREQAVPRDRIFSSRDGSAWRLTARSGGGNGVGTIPWTRFPISFDPGYVSL
                     HSSGRLFAIAGTSGTQGEYDGIYCSADGGRSWSKTCSE"
     gene            complement(7343..7876)
                     /locus_tag="VEV43_08435"
     CDS             complement(7343..7876)
                     /locus_tag="VEV43_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23587.1"
                     /translation="MSPGRGVTLAGPVAAGDASRATTVIQGQWFNGTGTTGCSGPLSA
                     LVCDHSADSGEPFAGASVCFEAVGGTGIGSLGCSASLSGSSVGIGKKDATCATRPANP
                     LSTNGTASYYSPTLGQGFSIPVKIFSDNGTTYFHGVGNFPLGTASIEGTYQAGCDVEG
                     VLWRGPFSGTFSIAIAV"
BASE COUNT         1198 a         2720 c         2836 g         1251 t
ORIGIN      
        1 agtcgacgcc gccctcgggg gccaacgtct cggtccggcg ggcgacgatc ttgagaccct
       61 cactgggggg cgcgtcgttc tcgagagggc cgttgccgct cgtcgtgaag ccgtcctcga
      121 tcgcgatgcc gacctggtcg atctctgcgc tgtcgagcgc ctcctcgacc tcgtcgtagc
      181 tcgggttcgg cggcccttcg gtgatctcct cgtcggtcgt gtggatgatg atgcgcctcg
      241 cgtcgggtcg gaacgtggcc gcctgtccgg ccgggacgac cgctccgacg ccgggtctgc
      301 tctcgccgcg ggcaacctgg tacagggcct cgagctgggt ctcctcgccg ccgccgaacg
      361 cgatcaggct ctcgagcgcc tcctcgaccg cgccgcagtt gttcgtgacg tctaggacgc
      421 gacggtacgc gggcggctgg tcgtagctgc ggtactcgcc gatgccgaac cagacgtcgt
      481 ggccgcggtt cgtcaggtgg gtgacgatct cgttcatggc gcgggcggtc ccgtcgatcg
      541 cgtcctgcat gctgccggac acgtcgatca agaagaagac gtcgacctgc tgccccttcg
      601 cggggaggtc gaagtcgaag tggacggtcc tgctctgacc gggcttgagc gtgaggtcga
      661 cgttcgccgg ctggagcgcg ggctcgccgg cgtcgtccag gtcgacgcac gggacctcga
      721 agtcctgctc ggtgtgggga cagccgggaa ggtctatgcc gttcttggcc gcggtcttgc
      781 agaggtcgaa cgtctgacgc gtcggcccgg cgaacacctt tctctcgatc gtggtctcgc
      841 tgcgacccca cagctcggtc cccgcgccgt ccgtgacgtg gaggtcgagg atcgggcggt
      901 ggtcggtggg cgtcaggtcg aacgcgggct gccccgacgt gagcagccac acgccggtgc
      961 tcgtcgagta cacctcctgg atcggcgaca tgatcacgag cgactgcgcg gccccgggaa
     1021 gggtggcgtc ggcgaagacg ctgcccccgt cggcggagcg atacacacgg ccgtcggtgg
     1081 tcgtcgcgat cacgcgcgcc ggttggcccg cctgatgcca gacgtccacg tccgagatcg
     1141 gcgcggggcg gggcgggtcg gaaggcgccg gtggcggaac ggtggacgct cgttcgatcc
     1201 ccttgtcgcc gtgcagccag acgacgtcgg cgtcgcgcgg gtcgacctct atgccgtccc
     1261 aggggtcgcc ggagtagaca ggcgtcatcg gcgtccacga cttgccgccg tcgacgctct
     1321 cgtacaggac ctgagcgacg cccttcgcgc cctggagctc gggaggaagg ccgtccggca
     1381 gcagcgatcg ttggatcccc gaggtgtcgg tcagcagaaa ggcccggccg gggttgctct
     1441 cggccgtggc gatctcctcc ggacggccga cggacgcctc gaggccgcgg tcggcgggct
     1501 cgaacgtctg cgctccgtcg ttgctgacga agacgcgggg gcgggagacc ggggcgccct
     1561 ggtcgaccgt gaccaggacc ttgttgggag cgagcgggga ctcggagacg tggacctgct
     1621 cgatgacgga ctcggtggag gtcgcggtcg tgccgttggc cgggacggcg tcgagccgcc
     1681 aggcaacgtt ccagctgcac ccccggtcga cggagcgcat gacgaccagg ccgttcgtcg
     1741 ccaccatcaa cgtcgtcgtc ttcgatatcg accaggactt gatcgcctgg gagccctcgg
     1801 ggaacgggac gtcgatgctc tcccacccac cgcggtcggc gccgcacaga ggtggagccg
     1861 aggtgccgtg cccgtgctgc gctacggcga agggggcgaa gaccgtcccg ccgaccatga
     1921 gcgtgaggac gatccggagc gcgcggctgt accagggcgt gcgggaccta gtcgcagtca
     1981 tgcgctcgta cactcactga cccttgtcgt gcggctccgc cggggcggaa tggcggctgt
     2041 tgctggcggt tccacggggg caaaacggtc gttcgttccc tcgtctgggt gacccatccg
     2101 aaggtaggtt cgttgtcgat tgcggcggtc ctgtttgcgg ccggacgagg ccaccggctg
     2161 aagcctctga cggacgtcgt ggcgaagccc gcgctgccgc tgttggacgt ccccctggcc
     2221 gccttcggcc tccggcgcct gttcgaagct gcccctgccg tcgtcgtgaa cgtacgccac
     2281 ctacccgagt cggtaacggc tgccgtagcc ccgtacgtac ccggggagtg ccgcctggat
     2341 gcgctcttcg aagcccccga agcgtacggg acggcaggca cgctcgtcgc cctccgggaa
     2401 cgattgggct cgccggtcct cacgtggaac gcggacgtgc tgacggacct gtcggcggta
     2461 tccctcctcg ccgcgcaccg ggcgtcgggg gccccggcga ccgtggcggt ggcggaggtg
     2521 tcctcggggg cggacttcgt cctcgagggg ggccgggcgg ccgggttcgt ggaccggcgt
     2581 tctcgggact ccgcggccgg gggcttgttc ctgggggtcg gcgtgttcga gcgaccggtc
     2641 ctgtcgtcgt tgcccgagac gcggccgctc ggcctcgggg agacgctgct ggcttctctc
     2701 tcgcggtccg gggacctggc ggtgttccgt cacgacgggt actggcgtga cgtcgggacg
     2761 ccggcggcgt atctggcggc gtcgctggag ctgctggacg gttcggggcc ggagcccccg
     2821 gacgggtcgt ggccgggaga aatcgtggcg ggctcctatc tcggtcgcgg tgcggtcgcc
     2881 gcgggtcgcg tcgagagatc ggtcctgctc ggcgggagcc gcgtcgaggc cggcgcccgg
     2941 gtcgagcggt ctctggtgtg gcgcgacgag gtggtgccgg cggggacggt gctccaagat
     3001 ggggtgtggt tcgggggccg ggcccttccc ttgtaggggc cggcaggacg aagggagcgt
     3061 ctccatgaag gtgcgcgcgg ctctggtcga ggcgctggtg cggttgtcgc tgctggtggt
     3121 cgcgggggcg ctgctgcggt gggcgacggt gacgctgagg gactggctcg aggacttcaa
     3181 cgcaactctc gagcgcacct ggggagggtg gttcgcgtcg ctggcgctgg ttgcagccgc
     3241 cggtttcctg gtgggcctcg ccgccctggc gggaagaccg cgcggctaca ggatcctgat
     3301 cccgctcgtg atcgcgttgc cgcctctggt tctgctcggc cactactacc tcgtgatcga
     3361 gaacgcggtg tcgaacggtg acgacctgcc ctggatcctc gggcactact tcttctacat
     3421 ggagcccgcg gcgcagtacg tgctggcctt ggtcgtggga ttcggggtcg catcggggtt
     3481 gcaacccgcc gcgcgtcagt cgtagaggcg gccggtctcc cgaaggcgta cctcgggcag
     3541 caggcagttc ccgtgcttcg acctagcggt cgtgacgtcg atgcgcgccc tgatgaaccc
     3601 cggcttgtgc cggtgggtga tcgtccagac gacggactcg cccggggtga ccgtcgcgcc
     3661 gagtgactgc ccggggtgga tcatccgggc gctgttgaat ccgggcacgt ggtagtcggc
     3721 gtagacgctc gcgccgtagc cctcgaagac gacgcggaag ccgtagtcgc ggtcgcaggt
     3781 ccagcgcaaa agcccgagcc gcggcagcgc gaggagaggg ccgcgcatcg tcggcccccc
     3841 gcgccacagg tcgacgtatc ggttcggctg gacggcggcc tcgagctcga gctcgtcgac
     3901 acgggcccgc gcctcggcga gccggtcctc gagaacggcg agccgcgcct gggcccgaac
     3961 gagcccggac gacgagcccg gcggacgctc gtcggagccg ccatcgcacg cggcgaggcc
     4021 gaacaagaag acggcgcaca acaggacgcg gcccattccc tgtagacgag aaggggggcc
     4081 gcgaagttcg gctggggagt cgctgctacc ggcaggtcgc ggcccaggtg cgcccgccgt
     4141 ccgtcgagca gtagagcccg ctgtggccac cgaggtcgcg cgccatgacg aagatcgtcc
     4201 cgtcccccgt caccgagacg aagttgttga cggggtagcc ccatgtcccg tcccacggga
     4261 tcgtcccccg ggggccgcgc tgttcggcat cgcgcgcgaa cgacagcagg tcccacgtcc
     4321 tgccgcgatc gcggctgacg aagaaccgct cgaccggtgc gtagcgatcc tccgcgtcgg
     4381 ccgggaggcg gtacccgagt cgcatgtaga tcgtgcgcgg tcgggtcgga tgaaggccga
     4441 gcccgatggt cggagtcgac ccgtcgagac gccggatcgg ccggagcatg cggttgacgc
     4501 tggcccacgg acggaaggtc gcgccgccgt ccttggacct gaacgccttg agggtcgaca
     4561 acccccgcat caacttcgac gtcgtcgcat agatacgctc gctctccgag aagtcgggcg
     4621 cgaaccagag cccgccgcgc ggctcgaact gataccccca cgggaatgcg aagagcggct
     4681 cggcacagac gagttgctcg ttgcaccgga acaacacgtt tcggctgcgc gacgtgatgc
     4741 cctgtgtgtt gtcgacctcg acggtcatcg tgtacgcgag ccgttcgccg tcgcgataac
     4801 cggggaacga gaacctccgc tcgtcgagag gcgctccggt gacagggatg cggtgtggcg
     4861 ggtcgatctt caccgacgcg tcgccggcgg cgagcgccag cacgggccgt tcaccctccg
     4921 gcgatcccgg caacccgacg gcgtcatcga cgaacgcgct gaggttgggg cccgaaggcc
     4981 cggtcgccgc gaagttgtcg acgagcgcga aggacgcccc gtcgtcgacg gtcgagaaca
     5041 cgccgcggtt ggtctggacg aagaccgtcc ggtccttcgc gtaaccgggc gaggcgatga
     5101 cgctgcccat gatctcgtcc gtcccagggg cgaaacccgt cgctgcgcgc cgagcccacg
     5161 tccggccgcc gtcagatgtc aggaagaacc tgaacgtcga cgcgtcgccg tcggaagtgt
     5221 cgaacgcgac gcagaagccc ttccggtcgg tgacgaactc aggcgagaaa tccgcgctga
     5281 tgcattgggg cgatgttgga gcctgccgag catggacgcc ccacgcggga gcggacaaaa
     5341 gcgtcagggc gagcacgacg gtcgaagccg tcaaacgtgt cacctgccgc gtcatccccc
     5401 cacctcgtcc gacgcccccg gcatgccgac gcttacgcag agttgagcac attgtgtatt
     5461 catcgtccat cgggatcaac gattgatcgt cgtcaaaggt cacgtgcgcg ccgcggttac
     5521 gaggctgaac tcgccttcga acgtgcctcg cgactgcagg atgctgcacg tcgtcgtgaa
     5581 gttgccgctc acctcgacga ccgtcagcaa gaagttgccg actccggcga acttccccac
     5641 gccccgcgac acccagacct ttacgggcac ggcgtaggac tgctgcgttg ccgagctgaa
     5701 gacggtcacc gttccgttgg tagtcgtgag ctggttcggc aacggcgtat tgaagcaggc
     5761 cgccttcttc agcttcccgg aggtcgtcgc gctcagcgag gccccgcact cgagcgacac
     5821 ggccaccgcc gccggcgtct ccgcgcaata agacccgggg gggaagtccg cgggggcttc
     5881 gccggtgaac gagcaccagt aatcgcccgt ggtcccggtc tcgtccgaac agtccgccca
     5941 cgagctgccg agccaggacc ccttgctgat cggcgcgcgc acgtcctcgg acgcgggcac
     6001 cggcgacgcg gacgtaccga agaggagcgt caccgccgcc gcgctcgtca cgaccagctt
     6061 cgacatcacg ttcatcccca cttcctttcg aaagccctta ttccgagcac gtcttggacc
     6121 acgatctccc accgtccgcc gagcagtaga tgccgtcgta ctcaccttgc gtcccagacg
     6181 tcccggcgat cgcgaacaac cgcccgctcg agtggaggga cacgtatccg gggtcgaaag
     6241 agatcgggaa cctggtccac gggatggtcc cgacgccgtt ccctccgccg gaccgtgcag
     6301 tcagccgcca cgcgctcccg tcgcggctgc tgaagatgcg gtcgcgcggc accgcctgtt
     6361 cgcgccggtc ttccgggtcg agtcccagcg aacgcgacac cctgagatag agacgttcgc
     6421 cgacggtccc cgtgacgccg acctcgacct gcgcgcccac cggaacgcgg cgaacgatgc
     6481 gatcgaccgc cgtccactcc gcgaacgtcg cgccgccgtc atgcgaccac cagaccgcgg
     6541 gtccatcttt ggccggctcg ctcagcacca ggacgacgac gtcgtcacca ccggcggatg
     6601 gaagccgcca ggcgcggatc gggatcggcc cccacgggaa ggcgtgcagc tgctgctgac
     6661 agacgaggtt cgcgtcacat ccgaagacga gcgtgcgagc gcgaggccgc tgcggatccg
     6721 acgaatccac ctcgaccgcg acggcgcgtg cgccctcggc ggatcgagcc tccggggaaa
     6781 caaggaagaa ccggtccgag aacggagacc cggggacgat ctgatggagc ggtggccgta
     6841 cgagtgcgga gacgtcgtcg ttcgcgaaag cgaacgccag cgacggattt ccgccgagct
     6901 cctccacgaa aggtgagagg ttccgccacg acaggcccga acccgcgagc gggtccgcga
     6961 gagagaacgt ctcacccccg tcttcgctgg cgaagagtcc ccggcccacc tggacgtaca
     7021 ccgtctcgtc ctcttcgaac cagggagaca ccacgaccgc gccgagcacg tctccgtcga
     7081 cgagctggac tccggccgcc gcgcggctgc cccacgacgt ccccgcgtcg tccgtccggt
     7141 agaggccgag atgcggcggg ccctgctcgg gaacggagac gtacgcgcag aacccgaagt
     7201 cgcccgagaa atgcaccgac tggcacgcga gtggcggctc cggggatgcc gcccgagtgg
     7261 gcgcagcgcc cgacagcagt aacaccgccg ccgccgaggc aatccgaacc ctgttcacga
     7321 tcgcccttgg cccccgacgt acctagacgg cgatcgcgat cgagaaggtg ccactgaagg
     7381 gcccgcgcca caggacgccc tcgacgtcac accccgcctg gtacgtcccc tcgatgctgg
     7441 ccgtccccaa ggggaagttg cccacgccgt gaaagtacgt cgtcccgttg tcggagaaga
     7501 tcttgacggg gatcgagaag ccctggccca gggtggggct gtagtagctc gccgttccgt
     7561 tggtcgagag ggggttggcc ggccgggtcg cgcaggtggc gtccttcttg ccgatcccga
     7621 cggagctccc ggagaggctc gcgctgcatc ccagcgatcc gatccccgtc ccgccgaccg
     7681 cttcgaagca gacggaagct cccgcgaacg gttcaccgga gtcggccgag tggtcgcaca
     7741 cgagtgcgct cagcggtccc gagcatccgg tggtgccggt cccgttgaac cactgcccct
     7801 ggatgaccgt cgtagcccgg cttgcgtccc cggctgcaac cggccccgcg agcgtcacgc
     7861 cccgcccagg tgacaccgtc cgcccctttc gcccattcgc ctcgggaaaa cgggagaaag
     7921 ttaacagcga gtcacctata tggcaaaccg tcggaaacgc tggggctagc cggcccccag
     7981 gaagaccgcg tcggccgcca tgtcg
//
LOCUS       DASQDO010000299        19378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_154193, whole genome shotgun sequence.
ACCESSION   DASQDO010000299 DASQDO010000000
VERSION     DASQDO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19378
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154193"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VEV43_08440"
     CDS             <1..1321
                     /locus_tag="VEV43_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYP23588.1"
                     /translation="IEYMREQRRRLGGFVPERRSKPRPVALPKQDLYEEFAAGSKQPV
                     STTMAFVRLLRKLMRDEKVGERVVPIIPDEARTFGMESLFPEFKIYAAHGQLYEPVDA
                     EHLLAYRESHQGQILEEGITEAGSMGSFTAAGTSYATHGESMIPFFIFYSMFGFQRIA
                     DSVWSFADQRGKGFLLGATYGRTTLNGEGLQHQDGHTLLMASTNPACLSYDPAYAYET
                     AVIVREGLRRMIDKDEDVFYYLTLYNENYLMPPMPEGVEEGIIRGIYKFKEAPGERKF
                     KAQIFGSGSIMSQALEAQNMLAKDHDVAADVWSVTSYQQLRADALETERWNRLHPEEA
                     ARQPYISKVLEQAEGPVIAVSDSMKAVPDQVSRWIPSPFIPLGTDGFGRSDHREALRR
                     FFEVDPEHVVVATLSALAQAGDVKPEAVTEALKKYGINPDAAPPNTV"
     gene            1627..3375
                     /locus_tag="VEV43_08445"
     CDS             1627..3375
                     /locus_tag="VEV43_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23589.1"
                     /translation="MHARRRSIEVAVSLAIALLAAAAPAASAPAVPPPTIEFLNPSSF
                     ALAADRGYVVSDQATDVGPDCCAASDEAFRFSAWVTGAPAESSVFFTVVQGALDFELP
                     AVRPSDSAPSSDTWEASWDIPPDVLNGPATATAYVVVDNEPIAQKSIEVTILRVSPGT
                     DLAYPQSGGSFGTFAPLATALPEDGPAEPTNPAGTVDALYSGTADMSYVRTFYTTSAP
                     GTEPVWHVCGTEPVGSNSNGEADNGVRCMTADAADAGRITAVAAVANNSPGDYDDRFN
                     QSGDAVRVVPYVQRPAALALDTPAQRVDKHAASERFFCSSTVTATVLDQLGRVVPGAN
                     VDAEAVGPTDALRFHTSSVLGGNVPPDRGAHSVETAFDCTGRAEVPPPNARPGKQGEH
                     QIFGLPDVKHIESAAGTSDRGTAGFRLYSSAPGLTSLNVWVDETDDGCSANDDRFTTG
                     EPSAAGSIGWGEDPPAPTPATPAALTSCHPDTRSARSVGIGVSDGAVAAGSEVRLSGR
                     VRAADGGCRSRQQVSLQARRPGRRFRSVARVVTTATGRYVFEITARQTRDYRAVVPAT
                     SACLGARSRSVRVATS"
     gene            complement(3450..4511)
                     /locus_tag="VEV43_08450"
     CDS             complement(3450..4511)
                     /locus_tag="VEV43_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23590.1"
                     /translation="MRKLIGLLALVTGATFLPATPAVAFHGSTAGLEAHCAGDTTASH
                     VVRNIYAYDMYDSALPEETYWNLIHEEITWADNGLRDSHSTYKQHWRFACERMVSFGA
                     LVRPRIEKVGIPTSEDVNGNLTIDCNEAQGHLTELGYYKTDQRFLFWFDGSISNCDTD
                     SVDEAWRFDEQTPSISPAEPGVDNPHNFVEGRAEFEWNDWDTLGVLDADHVAHEFYHS
                     IGAVEKDEHGDGHNCDAFWSGDGHPCGYYTGGHTRDYPDMMNTGYCVPKAADWDCHDH
                     DGPGDQTDDHAEIRLNCSNDAIVSGLQDPDMLMDCGRDSYWAVQFTSNNPWLCRHFNL
                     ATDSLYLWPRPTRPVDCPG"
     gene            4716..5072
                     /locus_tag="VEV43_08455"
     CDS             4716..5072
                     /locus_tag="VEV43_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP23591.1"
                     /translation="MAHRPVVLLVDDDPSMRELLATMFSMHGGFRIGGVACDGFEGAM
                     LAAAIEPDLVVLDFFMPRWDGSKAAEFIRQRCPRSKIIAFSAVLQEPPTWADDFLVKN
                     DVMELIPLAEQMATAA"
     gene            complement(5078..7729)
                     /locus_tag="VEV43_08460"
     CDS             complement(5078..7729)
                     /locus_tag="VEV43_08460"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYP23592.1"
                     /translation="MMRTLTKSLAALALSATVLVSPVGAADQSDPPVQPYGEGDGAIT
                     ALNILPPGQGEYQNGPEQAAGTQSPHNTDQIEPYERMVQGAPDIRKADLGQYFKDATF
                     GVKPDDIEREDSPRDGVVVQRDAGFGVPHVYGTTRADVMFGAGYVSAEDRLFMMDTLR
                     HVARGRMSEFLGASEANLASDRAVYRTSAYTEEELAAMIERVEGLHPELGPIAAADLT
                     AYVEGVNQYIDEARTDPRKLPAEYQALQIVPEDWLPTDTVAVASLIGSQLGVGGGDEL
                     GNAAFVGALQAKGFSFKQARRILADFNFPNDPESPVTTKKRFVWNTDLGKLDKRSRAF
                     PDDPAAVQEQLNAAVMPDRVDAPWGPIPLRFPDASSNALLVGSKKSSSGRPIAVFGPQ
                     VAYWSPQILMELDLHGPGIDARGVGFPGISQYVLLGRGADYAWSATSAGGDQVDTFVE
                     ELCDPEGGEATVDSSGYVADDGKCVEIYSRTDTWTAKPSAGGVPPENVVVEMTTERTD
                     SGIIQARGTVGDKPVAFTHLRSSFGKEIDSALTYVEISDPDRIDGAADFRRAFARFTF
                     TFNWFYLDDEQIAFQLGGLHPLRARRTDPDLPVWGTGKWDWRGRLSFAGTPYDVDPKK
                     GWMTSWNNKQAPGFRAHDGNFSYGPVQRVQMLDDGIRKYLKRKAKLSLVDLVNIMGDA
                     ATTDLRGTRVLPFMLKVVGKPSEPRLKEAVKLLKEWQRSGAHRRDKDADGSYEHSPAV
                     ALMDAWWEPAAQAIFKPVLGDAYDVLPMRHHDEPRAQGSAFQSGFYSHVNKDLRTVLG
                     LRVRGKASRVYCGEGSLKKCREALRASLEAAVAALEEEFGADPATWDADETGDQIDFT
                     PVGLQDQPNMQWQNRPTFQQVLEFGGR"
     gene            complement(7763..9712)
                     /locus_tag="VEV43_08465"
     CDS             complement(7763..9712)
                     /locus_tag="VEV43_08465"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYP23593.1"
                     /translation="MERREFLGRGLAASAAAIGATLFRLSPAQARAVQVVFADTFRRG
                     SSSKGWGKPWFNQRHGSPWGIAKKKGFYDLPEPQAMALEFAPNPVLVLDHDILDADVT
                     ATLSSTNANARFGLVARAVGYGDYYGAYLGPEGLTISRFMVNREEVLRRVPFAVSPKT
                     SYVVRLSVEGSSPVTLKAKAWKKGSQEPSRWTATASDNERDKRIEKAGAFGFVFMHDP
                     KTERAARVKVSKLKVESPQATKETPPRVAFSFAGRPQNGQIRLVAKTDTPAKEIRFQV
                     GTDPQLRRYKSIEPDEVLGKPGVAKAWAPAAAGGETMYWRVATKTKSGDRTRTRVHSV
                     RTPGAADEVSFGFGSCTHFWPVSRSFETAAGMNPNFFVHLGDLGYPQDAEGGAMAFRT
                     DAFQDRWTRMLARRGMARLHEKAHWIMLQDDHDYGADNAYRRDVRPFTVSAFDQISGN
                     LGSRHFDFRQGDVHAFFVDVHIDADHPLLDGPNPSLLGASQKSWLKQAMRASDASLLV
                     VFASMPLWGTGLGEGSWKEGFARERLELTEFFFEMQRSQRRVLICAGNAHAQYVNRHS
                     SPTGGKDLIEFVSSGTDRIDSTEATQLPINAADPVIDRTRSKKLIDAFGHVSLTGSGA
                     SRKVVLRAVSSKTGQNVWAPLELDL"
     gene            9976..10335
                     /locus_tag="VEV43_08470"
     CDS             9976..10335
                     /locus_tag="VEV43_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23594.1"
                     /translation="MAENAKVPARFGELVSELLRLTQEDRIHWSATDDPSTLMILRDA
                     GHVLLSSDAGAQPYRLALYAPSGEEVQSYTTPSTPQDHNEARLAHFIDQLWTVAGSSA
                     RNNDGLVTQFLEDLRNG"
     gene            complement(10368..11531)
                     /locus_tag="VEV43_08475"
     CDS             complement(10368..11531)
                     /locus_tag="VEV43_08475"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018852810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYP23595.1"
                     /translation="MPEAVIVSAARTPIGRAKKGSMLDVRPDDLGAFAVSAALKKVPE
                     VDPGEVVDVMIGCGFPEQKQGMNLGRRVALLAGLPEHVPGVTVNRFCASSLQTIRMAF
                     HAIRAGEGDVYVAGGVESISQVDGYPKDMDELHPKLFGDEAPIANVYIPMGMTAENVA
                     EQYDVSRDDMDRFAQRSQERAVAAQQSGFFEREISPYVKEDGTEVTTDDGPRASSTLE
                     KLKSLPPAFKPDGSVTAGNSCPLNDGGAAVVVMSDERASQLGVTPLARIIASSVSGVA
                     PEIMGVGPIEAVRKVLKQAGMSIDDVDVMELNEAFAAQVLPVCREVGIDPFGEKLNPN
                     GGAIALGHPYGMTGARIMCTLLNDLQSRDETIGLETMCVGGGQGMAMLIERLA"
     gene            complement(11582..14026)
                     /locus_tag="VEV43_08480"
     CDS             complement(11582..14026)
                     /locus_tag="VEV43_08480"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP23596.1"
                     /translation="MNKQLLRVLVASVSILAVVPTAAFAASPADAPDRSVEPIILTGA
                     QLPGWSGAPAEGVAEPYPSGAVVGERDAHNGQLVVPPAVGVNPDDVVAYAFDGDRFRE
                     IPVQVDERFPYFLANPNSDFGMYSGTDKELTYAWDVESWKKTGGRCFAEYPEEDTSKL
                     KGFPTTDPVPTLDADDEVVFMADDAGVQADLSQPGPPGTGDQRQEVRLVDPRDPAAER
                     FVYLFTKPTGSKFDADNGYVDYQRDANADEWIDRNSFAANDPEKLGTSNAGYGPNLSG
                     TVCNPDGTVRNSTDRFPRDGVTVTTDTYQWKATGRWMVREMHVTKPGTTRQFGPDLID
                     RWKGRAFQQSPESTISVVGFEDEQVNWEANSSLLGELEGPVRAIREVWGADSGTNVTK
                     TETFYRDAITYRYKVRVHPIPPDGLYTSWDYNHDQVATYYNETKTEGVAIDGVNDDIL
                     NVDSLPNGQPAFFDAPDPTFTKPLAFLNWEQVSGRGDNGSLVYLFEMKNAQALENPTV
                     VPYYRDDACLDDGTGDNPSPRLNPGDPDPRNLPCYTEAPAGYDGPYMQGAFGSHGVHY
                     FFTGDTDNAVTGAPVTEIDGQQWQWAVPTEAPKNVGDRYANTVKTPLAPVVTLQPSTA
                     GQAEPTSLEITGATSGQTTDEVVLEATLRSGDAGVEGQTISFTFDGAEVGDAVTDAAG
                     RASLPVTLEGPARTTEVSAAFAGSDDYAESAGQSPFEVQREDTSLQVTFDTTSAPARA
                     VALLTDADSGAPLAGKQVSFTVNGLSFGTHTTAADGTATILLPKPKARALQVTASFAG
                     DDSFNSASDTETLQKS"
     gene            complement(14107..14280)
                     /locus_tag="VEV43_08485"
     CDS             complement(14107..14280)
                     /locus_tag="VEV43_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23597.1"
                     /translation="MRRIEDFANQGVVGAADPQLGYVAPDVLVWPEGLHPAARDLQDS
                     AADLVLSTNGLTR"
     gene            complement(14360..14791)
                     /locus_tag="VEV43_08490"
     CDS             complement(14360..14791)
                     /locus_tag="VEV43_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYP23598.1"
                     /translation="MADTPDPAVLAEAIKDLVDAALAEQIRGIGTEEVLRAIFSGMED
                     AFVPERAAGVDSTIQYDISTEDGTKTWSVSFAGGKCTTSEGPATNPRLTLQIGLVDFI
                     RLIFGQAQGPQLFMTGKIKLQGDMMFAMQMQGFFNAPGAAK"
     gene            14898..16805
                     /locus_tag="VEV43_08495"
     CDS             14898..16805
                     /locus_tag="VEV43_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HYP23599.1"
                     /translation="MGESHVCKSILEGLNPEQTEAVRAVRGPVCILAGAGSGKTTTIT
                     RRIAYQVATGTFRGGEVLAVTFTDKAAGEMRSRLAALGADGVRARTFHAAALAQLRHL
                     APTEPPRILPSKAVMLRQIGNTLPKPYRFRPAGDLATEIEWAKTRRLSPEDYARSLGG
                     HVPPIPADLMTGVYREYERRKTREGAMDFEDLLEATIRMFAEDEWALERFHQRYRAFT
                     VDEYQDVNLLQQTLLETWVGDRDDLCVVGDDYQSIYSFTGASPRYLLEMPRKWPRVKV
                     VRLEENFRSTPQVLTMANRLVPKLGGAEKTLRPVREDGPAPVVAPFVDRGSETKFLVD
                     KIAELHAAGVPYEEMAVLYRVNFRSEDYEDALGAARIPFQVRDGAFLTRIAARRLLST
                     LRRSKVNDVADEVLRVAKRDGFQERPPEGLGDQETTRQNDLARLVRLAREFDGSTAAE
                     FVAHVEGRFASDGEGHGVHLLTLHRAKGLEFEAVFLPRLEEGELPGKRSKTDEAIAEE
                     RRLLYVGLPRAKRHLYLTWTTGGRQSASRFVEELGGCLVPHPLRQTKRRPEAAHDPVY
                     QALKAWRLERAKRDGVPAYVIFHDTTLQEIAEASPRSIVQLAHISGVGPAKLERYGNE
                     VIVTLHEAVAS"
     gene            16867..17418
                     /locus_tag="VEV43_08500"
     CDS             16867..17418
                     /locus_tag="VEV43_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HYP23600.1"
                     /translation="MRLAPALLLLFAACSGHRVDLTVVAPATLHLEPPGYEEAVVSRA
                     VDGDTIEVEVTGRVEGPGAGEAVVGETYVVRLLGIDTPESVSPREPVECFGREASAAT
                     AALLQGTTVKLVKDVEERDSFDRLLRYVYMGAELANARLVTNGYAHAYTYPPNVRHAQ
                     LLSEVERAARDGGRGLWAPEACP"
     gene            complement(17415..17834)
                     /locus_tag="VEV43_08505"
     CDS             complement(17415..17834)
                     /locus_tag="VEV43_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23601.1"
                     /translation="MRPKVAPLRLTVALVLAGAALSLGSQAAADCAAPSVHVSPRRVV
                     SGDELSVTGDSWGDACNDTPGPVCNPPPLGHPIEDVRLGLWAAGEGSVHLRTLDADDE
                     YSFDATVEVPDVPPGTYSLRATGDEHVADARVVVVPD"
     gene            complement(17831..18640)
                     /locus_tag="VEV43_08510"
     CDS             complement(17831..18640)
                     /locus_tag="VEV43_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HYP23602.1"
                     /translation="MRWILAALTSLLVGACGGPPSDAGSASSPSHDDPAARSVSYDGT
                     WELVEGYAPEGPVEISERFRITLTINGGRWGGLSACNYYGASATVDGTSISVGGIGGT
                     EMGCRPEAAEREARYQAALMAAETIERSGERLTLTGPDAALVFEFVPPPPTAKLTGVR
                     WELESIVHGRGPDAATSEARPAFLYFDKDGKFEGSTGCRGLKGEWAEEADRIMFLSLS
                     ADDGQCSDAMTAQNDAVFTVTDGFTFEIEGDTLTAYGRFSDTALVYRARSS"
     gene            complement(18778..19338)
                     /locus_tag="VEV43_08515"
     CDS             complement(18778..19338)
                     /locus_tag="VEV43_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HYP23603.1"
                     /translation="MDGLEFPTQAAFERWLAEDHAKSDGIWLKIAKKGARKKTVTYAE
                     AVEVALCYGWIDGQMKRLDDEYFVQRFTPRRARSKWSKINVGKVEDLIARGKMKPPGL
                     AEVERAKADGRWAAAYDSPSTATVPDDLAAALEAKPAAKELFEKLTSSQRYSILYQLQ
                     DAKRAETRARRIAKFVDMLDRGDRPG"
BASE COUNT         2881 a         6637 c         6670 g         3190 t
ORIGIN      
        1 gatcgagtac atgcgcgagc agcgccggcg tctcggcggg ttcgtcccgg agcggcggtc
       61 gaagccgcgg ccggtagcgc tcccgaagca ggacctgtac gaggagttcg cggcggggtc
      121 gaagcagccg gtgtcgacga cgatggcgtt cgtgcggctg ctgcgcaagc tcatgcgcga
      181 cgagaaggtg ggggagcgcg tcgtcccgat catcccggac gaggcccgca cgttcggcat
      241 ggagtcgctg ttccccgagt tcaagatcta cgcggcgcac ggccagctct acgagccggt
      301 cgacgccgag cacctgctcg cgtaccgcga gtcgcaccag ggtcagatcc tcgaagaggg
      361 gatcaccgag gccggctcga tgggctcgtt caccgcggcc ggcacgtctt acgctacgca
      421 cggcgagtcg atgatcccgt tcttcatctt ctattcgatg ttcggcttcc agcggatcgc
      481 cgactcggtc tggtcgttcg cggaccagcg cggcaagggc ttcctgctcg gcgcgacgta
      541 cggccgcacg acgctcaacg gcgaggggct ccagcaccag gacggccaca cgctgctgat
      601 ggcgtcgacg aaccccgcgt gcctgtccta cgaccccgcg tacgcgtacg agaccgcggt
      661 gatcgtgcgc gagggactgc gccggatgat cgacaaggac gaggacgtct tctactacct
      721 gacgctctac aacgagaact acctcatgcc gcccatgccg gaaggcgtcg aggaagggat
      781 catccgcggc atctacaagt tcaaggaggc gccgggcgag cgcaagttca aggcgcagat
      841 cttcgggagc ggttcgatca tgagccaggc cctcgaggcc cagaacatgc tggcgaagga
      901 ccacgacgtg gccgcggacg tgtggagcgt gacgtcgtac cagcagctgc gtgccgacgc
      961 gctcgagacc gagcgctgga accgtctgca ccccgaagag gcggcccggc agccgtacat
     1021 ctcgaaggtc ctagagcagg ccgagggccc ggtgatagcg gtgtcggact cgatgaaggc
     1081 ggttccggac caggtctcgc gctggatccc gtcgcccttc attccgttgg gcacggacgg
     1141 ctttgggaga tcggaccacc gcgaagcgct gcgccgtttc ttcgaggtag acccggagca
     1201 cgtggtggtg gcgacgctct cggccctggc ccaagcgggc gacgtgaagc cggaagccgt
     1261 gacggaagcg ctgaagaagt acgggatcaa ccccgacgca gccccgccga acaccgtctg
     1321 atcccgacaa cgcactagca gcgcggtggc acgatcccga caccgcgctc gcctaagccg
     1381 atggtgtgac gacgacctcg cgtcgacgcg cgtgacaggc gtcgaccttt gcgccccgcg
     1441 cgttttgatc cgctttcgac ctagttcctt cgtctactag agacagtcgc cggacattag
     1501 gcggccagtc tcttgatcgc gcttgcggtt ggttgatgaa tgcatcgcac agggccaacg
     1561 gatgcgcgcg gctgcacgac agtgccgaca gtcgcgccgt ccgagcgaga aagacgaggg
     1621 actgacatgc acgcacgccg acgttcgatc gaagtagccg tctcgctggc gatcgcgctc
     1681 cttgcggccg ccgctcctgc tgcgtccgcg cccgccgtgc cacccccgac gatcgagttc
     1741 ctcaacccgt cgagcttcgc cctcgccgcg gatcgtggct acgtcgtgtc cgatcaggcg
     1801 acggacgtcg ggccggactg ttgcgcggcg tccgacgagg ccttccgctt cagcgcgtgg
     1861 gtcacgggcg cgcccgcgga gtcgagcgtc ttcttcacgg tcgtgcaagg cgcgctcgac
     1921 ttcgagcttc ccgcagtgcg gccttccgac tccgcgccgt cgtccgacac gtgggaggcg
     1981 tcgtgggaca tcccgcccga cgtgctcaac ggaccggcta ccgcgacggc atacgtcgtc
     2041 gtcgacaacg aaccgattgc gcagaagagc atcgaggtca ccatcctgcg cgtgagcccc
     2101 gggaccgatc tggcgtaccc gcagagcgga ggctcgttcg gcacgttcgc gccgcttgcc
     2161 accgccttgc ccgaggatgg gccggccgag ccgaccaacc cggccggaac cgtggacgcc
     2221 ctctacagcg gcaccgccga catgtcgtac gtgcggacct tctacacgac gtcggctccg
     2281 gggaccgagc cggtctggca cgtgtgcggg acggaacccg tcggaagcaa ttcgaacggc
     2341 gaggccgaca acggcgtccg gtgcatgacc gccgatgcgg cggacgccgg ccggataacg
     2401 gcagtggccg cggtcgccaa taactccccg ggcgactacg acgaccgctt caaccagtcg
     2461 ggcgacgccg tacgcgtggt gccgtatgta cagcggcccg cggcgttggc cctcgacacg
     2521 cccgcacagc gcgtcgacaa gcacgcggcc tcggaacgct tcttctgcag ctcgacggtg
     2581 accgcgacgg tcctcgatca gctcggacgg gtcgtcccgg gcgcgaacgt cgacgccgaa
     2641 gccgtcggac cgacggacgc gctcaggttc cacacgtcgt cggtcctcgg cggcaacgtc
     2701 ccaccggacc ggggcgcaca ttccgtcgag acggccttcg actgcacggg cagagccgag
     2761 gtccctccgc ccaacgccag gccgggtaag cagggagagc atcagatctt cggcctgccc
     2821 gacgtgaagc acatcgagtc ggccgccggc acgagcgacc gtgggacggc ggggttcagg
     2881 ctctactcga gcgcacccgg cctcacgagc ctcaacgtgt gggtggacga gaccgacgac
     2941 ggttgttccg ccaacgacga ccgtttcacg accggcgagc cgtcggcggc cgggtcgatc
     3001 ggatggggtg aggatccgcc ggcaccaact ccagcgaccc cggctgcatt gacgtcgtgt
     3061 caccccgaca ctcggtcggc gaggtctgtg ggcatcggcg tctcggatgg cgcagtcgcc
     3121 gcaggctcgg aggtgaggct ctccggacgc gtgcgcgcgg cggacggcgg ctgccgttcg
     3181 cggcagcagg tgtcgctgca ggcacggcgg ccgggccggc ggttccgtag cgtcgcgcgc
     3241 gtggtgacga cggcgacggg ccggtacgtc ttcgagatca cggcccggca gacgagggac
     3301 taccgcgcgg tcgtcccggc cacctccgcg tgcctcggcg ctcgatcccg gtccgtcagg
     3361 gtcgcgacct cgtaagggcc cgaagcagta gtcgaatcaa gagaggcccc cgcagtgcgg
     3421 gggcctctct tgatagcgcg tcttgccggt tagccggggc agtcgacggg ccgcgtcgga
     3481 cgcggccaca ggtagagcga atcggtggcg aggttgaagt gccggcagag ccacgggttg
     3541 ttcgacgtga actgcacggc ccagtagctg tcgcgcccgc agtccatcag catgtccgga
     3601 tcttggaggc ccgacacgat ggcgtcattc gagcaattga gccttatctc cgcgtggtcg
     3661 tcggtctggt cgcccggccc gtcgtgatcg tggcagtccc agtccgcggc cttcgggacg
     3721 cagtaccccg tgttcatcat gtcggggtag tcgcgggtgt gtcccccggt gtagtagccg
     3781 cacgggtgcc cgtcgcctga ccaaaacgca tcgcagttgt gaccgtcgcc gtgctcgtcc
     3841 ttttctacgg ccccgatcga gtggtagaac tcgtgtgcga cgtggtcggc gtcgaggact
     3901 cctaaggtgt cccagtcgtt ccattcgaac tcggcgcgtc cctcgacgaa gttgtgcggg
     3961 ttgtcgactc cgggctcggc gggagagatg gacggggtct gctcgtcaaa cctccacgcc
     4021 tcgtcgactg agtcggtgtc gcagttcgag atcgagccgt cgaaccagaa gaggaaccgc
     4081 tggtcggtct tgtaataccc gagctctgtg aggtggccct gggcctcatt gcagtcgatc
     4141 gtcaagttgc cgttgacgtc ctccgaggtc gggatcccga ctttctcgat ccgcggacga
     4201 accagtgctc cgaaagagac cattcgctcg cacgcgaagc gccagtgctg cttgtacgtc
     4261 gaatgggagt cgcgcagccc gttgtccgcc cacgtgatct cctcgtggat caaattccaa
     4321 tacgtctcct cgggcagggc gctgtcgtac atgtcgtacg cgtagatgtt cctgacgacg
     4381 tgtgaggcgg tcgtgtcacc ggcgcagtgc gcctcgagcc ccgccgtcga gccgtggaaa
     4441 gcgacggccg gcgtcgccgg caggaatgtc gcgcccgtga cgagcgcgag caggccgatc
     4501 agtttcctca tgaaggtccc cccactgtgt agacgtccgc acctccatcg gccctacccg
     4561 gttctcccgg aggtacacag gtcgatcaag cagtccaatc ccgagggctc gatctgctca
     4621 ccatccggtg tcatgcccga ttgatcccgc aacgaccgac atatagctag tttgggctat
     4681 tgccgctgga cgcggccggg cgcagcattt gtcctatggc gcacagaccg gtggttctgc
     4741 tcgtagacga cgatccctcg atgcgagagc tcctcgcgac gatgttcagc atgcacggcg
     4801 gcttccgcat cggcggcgtc gcttgcgacg gcttcgaggg cgcgatgctc gccgccgcca
     4861 tcgagcccga cctcgtcgtg ctcgacttct tcatgccccg ctgggacggg agcaaggcgg
     4921 ccgagttcat ccgccagcgc tgcccccgca gcaagatcat cgccttctcg gccgtgctcc
     4981 aggagccccc gacgtgggcg gatgacttcc tcgtgaagaa cgacgtgatg gagctgatcc
     5041 cgctggccga gcagatggcg accgcggcct aaccggctta acggcccccg aactcgagca
     5101 cctgctggaa cgtcggccgg ttctgccact gcatgttcgg ctggtcctgt agccccaccg
     5161 gcgtgaagtc gatctgatcg ccggtctcgt ccgcgtccca cgtcgcgggg tcggcgccga
     5221 actcctcctc cagcgccgca accgccgctt cgaggctcgc ccgcagcgcc tcccggcact
     5281 tcttcagcga accctccccg cagtagaccc tcgacgcctt gccgcgcacg cgcagaccca
     5341 gcaccgtccg caggtccttg ttcacgtggc tgtagaagcc gctctggaac gcgctgccct
     5401 gggccctcgg ctcgtcgtga tggcgcatcg gcaagacgtc gtacgcgtcg ccgaggacgg
     5461 gcttgaagat tgcctgcgca gccggctccc accacgcgtc catcagcgcg acggcagggg
     5521 agtgctcgta cgaaccgtcg gcgtccttgt cccgccggtg cgcgcccgaa cgctgccact
     5581 ccttcagcag cttcaccgcc tccttgaggc gcggctcgga cggcttgccg acgaccttca
     5641 gcatgaacgg gaggacgcgg gtcccccgca ggtcggtagt ggcggcgtcg cccatgatgt
     5701 tgacgaggtc gacgaggctc agcttcgcct tgcgcttcag gtatttgcgg atcccgtcgt
     5761 cgagcatctg cacgcgctgg acggggccgt agctgaagtt gccgtcgtgc gcacggaagc
     5821 ccggtgcctg cttgttgttc caggacgtca tccaaccctt cttcgggtcg acgtcgtacg
     5881 gcgtcccggc gaacgagagc cgcccgcgcc agtcccactt gcccgtcccc cacaccggca
     5941 ggtccggatc ggtacggcgg gcacgcaggg gatggagccc cccgagctgg aacgcgatct
     6001 gctcgtcgtc caggtagaac cagttgaacg tgaacgtgaa gcgggcgaac gctcgccgga
     6061 agtccgcggc cccgtcgatc ctgtcggggt ccgagatctc gacgtacgtg agcgcggagt
     6121 cgatctcctt gccgaacgac gaccgcaggt gcgtgaacgc gaccggcttg tcgccgacgg
     6181 tcccgcgggc ctggatgatc ccggagtcgg tcctctcggt cgtcatctcg accacgacgt
     6241 tctcgggcgg gaccccgccg gccgacggct tcgcagtcca ggtgtccgtc cgcgagtaga
     6301 tctcgacgca cttcccgtcg tccgcgacgt agccggagct gtcgacggtg gcctcaccgc
     6361 cctcggggtc gcacagctcc tccacgaacg tgtcgacctg gtcgcctccg gcagaggtcg
     6421 cgctccacgc gtagtccgcg ccgcggccca acaagacgta ctgcgagatg ccggggaagc
     6481 ccacgccgcg cgcgtcgatc cccggcccgt ggaggtcgag ctccatcagg atctgcggag
     6541 accagtacgc gacctggggg ccgaagaccg cgatggggcg gcccgacgag gacttcttcg
     6601 atccgacgag gagcgcgttg ctcgacgcgt ccgggaaccg cagcgggatc ggcccccacg
     6661 gcgcgtccac gcggtcgggc atgaccgcgg cgttcagctg ctcctgcacc gcggcggggt
     6721 cgtcggggaa cgcccgtgag cgcttgtcga gcttgccgag atcggtgttc cacacgaatc
     6781 gcttcttcgt ggtgacgggc gactcggggt cgttcgggaa gttgaagtcg gcgagtatcc
     6841 gccgcgcctg cttgaacgag aaccccttgg cctgcagcgc tccgacgaac gccgcgttgc
     6901 ctagctcatc gcccccgccg acgccgagct gagacccgat gaggctcgcg acggcgacgg
     6961 tgtccgtcgg gagccagtcc tcggggacga tctgcagcgc ctggtactcg gccggcagct
     7021 tcctaggatc ggtccgcgcc tcgtcgatgt actggttgac cccttccacg tatgcggtca
     7081 ggtcggcggc tgcgatcggg ccgagctccg ggtgcagccc ttcgactcgc tcgatcatcg
     7141 cggcgagctc ttcttcggtg taggccgagg tccggtagac agcacggtcg ctcgcgaggt
     7201 tcgcctcgga cgcgccgagg aactcgctca tccgtccgcg cgcgacgtgc cgcagcgtgt
     7261 ccatcatgaa cagacggtct tcggccgaga cgtatcccgc gccgaacatg acatccgcgc
     7321 gcgtggtccc gtagacgtgc ggtactccga agccagcgtc gcgctggacg acgactccgt
     7381 ctcgcggcga gtcctcgcgc tcgatgtcgt ccggcttcac gccgaaggtc gcgtccttga
     7441 agtactggcc taggtcggcc ttgcggatgt ccggcgcgcc ctggaccatc cgctcgtagg
     7501 gctcgatctg gtcggtgttg tgcgggctct gcgtcccggc cgcctgctcg ggcccgttct
     7561 ggtactcgcc ctgacccgga gggaggatgt tgagcgcggt gatcgccccg tcgccctcgc
     7621 cgtaaggctg gaccggggga tcggattggt cggcggcgcc gacgggtgag acgaggactg
     7681 tcgcggacaa ggcgagcgcc gcgaggctct tcgtaagggt tcgcatcatc ccaccctacg
     7741 gcgctaccga acgaaggacg gtctagaggt cgagctccag gggggcccag acgttctgcc
     7801 ccgtcttcga ggacaccgcc cgcagcacga ccttgcgcga ggcccccgag ccggtgaggc
     7861 tcacgtgccc gaacgcgtcg atcagcttct tgctgcgcgt ccggtcgatc acggggtccg
     7921 cggcattgat cgggagctgc gtcgcctcgg tcgagtcgat ccggtcggtc cccgaagaca
     7981 cgaactcgat caggtccttg cccccggtcg ggcttgagtg ccggttcacg tactgcgcgt
     8041 gcgcgttccc cgcgcagatc agcacccggc gctgcgacct ctgcatctcg aagaagaact
     8101 cggtgagctc aaggcgctcg cgcgcgaagc cctccttcca cgagccttcg ccgagacccg
     8161 ttccccacag cggcatcgac gcgaagacga cgaggagcga cgcgtcggac gcgcgcatcg
     8221 cttgcttcag ccacgacttc tggctcgcgc ccagcaggct cgggttcggc ccgtcgagca
     8281 gtgggtggtc ggcgtcgatg tgcacgtcga cgaagaacgc gtggacgtca ccctggcgga
     8341 agtcgaagtg gcggctccct aggttgcccg agatctggtc gaacgcgctc acggtgaacg
     8401 gccgcacgtc gcgccggtag gcgttgtcgg ccccgtagtc gtggtcgtcc tggagcatga
     8461 tccagtgcgc cttctcgtgc aggcgcgcca tgccgcggcg ggcgagcatg cgcgtccacc
     8521 ggtcctggaa cgcgtcggtg cggaacgcca tcgcgccgcc ctccgcgtcc tgcgggtagc
     8581 cgaggtcgcc gaggtgcacg aagaagttcg ggttcatgcc cgctgcggtc tcgaacgacc
     8641 ggctcaccgg ccagaagtgc gtgcacgagc cgaagccgaa cgagacctcg tcggccgcgc
     8701 cgggcgtgcg gacggagtgc acccgggtgc gcgtgcggtc gcccgacttc gtcttggtcg
     8761 ctacgcgcca gtacatcgtc tcgccgccgg cggcggccgg ggcccatgcc ttcgcgacgc
     8821 cgggtttacc gaggacctcg tcgggctcga tcgacttgta gcgccggagc tgcgggtcgg
     8881 tcccgacctg gaagcggatc tctttggccg gcgtgtcggt cttggcgacg aggcgtatct
     8941 ggccgttctg cggacggcct gcgaacgaga acgcgacgcg cggcggggtc tccttcgtgg
     9001 cctggggcga ctcgaccttg agcttagaga ccttgacgcg ggcagctcgc tcggtcttgg
     9061 gatcgtgcat gaagacgaag ccgaaggccc cggccttctc gatccgcttg tcgcgctcgt
     9121 tgtccgatgc agtcgcggtc cagcgggacg gctcctgcga gcccttcttc cacgccttgg
     9181 ccttcagggt gaccgggctg cttccctcga cggagagccg gacgacgtaa gacgtcttgg
     9241 gggagacggc gaacgggacg cgacgcagga cctcttcgcg gttgaccatg aagcgcgaga
     9301 tcgtcagccc ctcggggccg aggtatgcgc cgtagtagtc cccgtagccg acggctcggg
     9361 cgacgagccc gaaccgtgcg ttggcattgg tcgagctcag cgtcgccgtg acgtcggcgt
     9421 cgaggatgtc gtggtcgagg acgaggacgg ggttcggggc gaactcgagc gccatggcct
     9481 ggggctcggg gaggtcgtag aagcccttct tcttggcgat cccccagggg gagccgtggc
     9541 gctggttgaa ccagggcttg ccccagccct tcgagctcga tccgcgccgg aaggtgtcgg
     9601 cgaagacgac ctggacggcc cgcgcctggg ccgggctgag ccggaagagg gtggccccga
     9661 tagctgccgc agaagccgcg agcccgcggc cgaggaattc acgccgttcc acacagtcct
     9721 atcggcggtt ccttccccca cctcaagcaa acgacggcag gatcgtgtga cacgcaccac
     9781 cttcgcgcga cggacgatcg ctatcttgtt gcgatgttcg gctcgatcag atccggagcg
     9841 acctcacact ggatctttgg cggtgcgggc gtcctcgtcc tgtcgtgggg gctcatcgcg
     9901 gccgtagaag cacgggcgcc atatgtgacg acgttccgga gctcaaggct cgtctagcca
     9961 ggtcaggagg cgcagttggc tgagaacgcg aaagtccctg cacgattcgg cgagctcgta
    10021 tccgaactgc tcaggctgac ccaagaggac cggattcact ggagcgcgac cgacgacccc
    10081 tcgacgctca tgatcttgcg cgacgcgggc catgtcttgc tctccagcga cgccggcgcc
    10141 cagccctacc ggctcgccct gtacgcaccg tcgggtgagg aggttcagag ctacacgacg
    10201 ccgtcgacgc cccaggatca caacgaggcg cggttggcgc acttcatcga ccagctatgg
    10261 accgttgctg ggtcgtctgc gcggaacaac gacggactgg tcacgcagtt tctggaggac
    10321 ttgcgtaacg gatgagggcg gtggcctagg ccagccccgc ggatctccta ggccagcctc
    10381 tcgatcagca tcgccatgcc ctggcccccg cctacgcaca tcgtctccag acctatcgtc
    10441 tcgtccctcg actgcaggtc gttcaggagc gtgcacatga tcctggcccc cgtcatgccg
    10501 taggggtggc ccagcgcgat cgcaccgccg ttcgggttca gcttctcgcc gaacgggtcg
    10561 atcccgacct cccggcacac cggcaggacc tgcgcggcga acgcctcgtt cagctccatc
    10621 acgtcgacgt cgtcgatcga catcccggcc tgcttcagga ccttgcggac ggcttcgatc
    10681 ggcccgacgc ccatgatctc cggagcgacg cccgacaccg acgaggcgat gatccgggcc
    10741 aggggagtga cgcccagctg agacgcacgc tcgtcgctca tcaccacgac cgcggccccc
    10801 ccgtcgttca gcgggcacga gttcccagcc gtcaccgagc cgtccggctt gaacgcgggc
    10861 gggagcgact tgagcttctc gagagtcgag ctcgcccgcg gcccgtcgtc cgtcgtcacc
    10921 tcggtcccgt cctctttgac gtagggcgag atctcgcgct cgaagaagcc cgactgctga
    10981 gccgcgacgg cgcgctcctg cgaacgctgc gcgaaccggt ccatgtcgtc gcgcgacacg
    11041 tcgtactgct cggcgacgtt ctcggccgtc atccccatcg ggatgtacac gttcgcgatg
    11101 ggggcctcgt cgccgaacag cttcgggtgc agctcgtcca tgtccttcgg gtagccgtcg
    11161 acctgagaga tcgactcgac gccgccagcg acgtagacgt caccctcgcc cgcacggatc
    11221 gcgtggaacg ccatgcggat cgtctggagg ctcgacgcgc agaagcggtt aacggtcacg
    11281 cccggcacgt gctcgggcag ccctgcgagc agcgcgacgc ggcggcccag gttcatgccc
    11341 tgcttctgct ccgggaagcc gcagccgatc atcacgtcga cgacctcgcc ggggtcgacc
    11401 tccgggacct tcttcagcgc ggccgagacc gcgaacgcac cgaggtcgtc ggggcggacg
    11461 tcgagcatcg accccttctt ggcgcggccg atgggggtcc gggcggcgga gacgatgact
    11521 gcttcgggca tgggggctcc tcgggataga cgcttctccg agaaacccta atccggggcc
    11581 gtcagctctt ctgcaaggtc tccgtgtcgc tggccgagtt gaacgagtcg tctccggcga
    11641 acgaggcggt gacctgcagg gccctcgcct tcggcttcgg caggaggatc gtcgccgtcc
    11701 cgtcggcagc ggtcgtgtgc gtcccgaacg agagcccgtt gacggtgaac gacacctgct
    11761 tcccggcgag gggagctccg ctgtccgcat ccgtaaggag cgccacggcc ctcgccggcg
    11821 cggaagtcgt gtcgaacgtc acctggagcg aggtgtcctc acgctggacc tcgaacgggc
    11881 tctgacccgc ggactcggcg tagtcgtcgg agccggcgaa agcggccgag acctcggtgg
    11941 tgcgggccgg gccctcgagc gtcaccggca gggaagcgcg gcctgccgca tccgtgacgg
    12001 cgtcgccgac ctcggctccg tcgaacgtga acgagatcgt ctgcccctcg accccggcgt
    12061 cgccggagcg cagcgtcgcc tcgagcacga cctcgtcggt cgtctgaccg gaggtggcgc
    12121 cggtgatctc gagcgacgtc ggctcggcct ggcccgccgt gctgggctgc aaggtcacga
    12181 cgggcgcgag cggcgtcttc acggtgttcg cgtaacggtc gccgacgttc ttgggggcct
    12241 ccgtcgggac ggcccactgc cactgctgac cgtcgatctc ggtgaccggc gcgccggtga
    12301 ctgcgttgtc ggtgtcaccg gtgaagaagt agtggacgcc gtggctgccg aacgcgccct
    12361 gcatgtaggg accgtcgtag cccgcgggag cctccgtgta gcaggggagg ttgcgggggt
    12421 cggggtcgcc ggggttgagg cggggcgacg ggttgtcgcc ggtgccgtcg tcgagacacg
    12481 cgtcgtcacg gtagtagggc acgacggtcg ggttctcgag cgcctgcgcg ttcttcatct
    12541 cgaagaggta gacgagcgag ccgttgtcgc cgcggcccga cacctgctcc cagttcagga
    12601 acgcgagcgg cttcgtgaac gtcgggtccg gagcgtcgaa gaacgccggc tgaccgttcg
    12661 ggagcgagtc gacgttgagg atgtcgtcgt tgacgccgtc gatcgccacg ccttcggtct
    12721 tcgtctcgtt gtagtaggtc gcgacctggt cgtggttgta gtcccacgac gtgtagaggc
    12781 cgtcgggcgg gatcgggtgc acgcggacct tgtaccggta ggtgatcgcg tcgcggtaga
    12841 aggtctcggt cttcgtcacg ttcgtgccgg agtcggcgcc ccacacttcg cggatggcgc
    12901 gcaccggccc ctcgagctca cccagcagag acgagttcgc ctcccagttc acctgctcgt
    12961 cctcgaagcc gacgaccgag atcgtcgact cggggctctg ctggaacgca cggcccttcc
    13021 acctatcgat gaggtcgggg ccgaactggc gcgtcgtccc gggcttggtc acgtgcatct
    13081 cgcggaccat ccaccggccg gtcgccttcc actggtaggt gtcggtcgtg accgtgacgc
    13141 cgtcgcgcgg gaagcggtcg gtcgagttgc ggaccgtgcc gtcggggttg cacacggtgc
    13201 cggagaggtt cgggccgtag ccggcgttcg acgtgccgag cttctcgggg tcgttcgccg
    13261 cgaagctgtt gcggtcgatc cactcgtcgg cgttggcgtc gcgctggtag tcgacgtatc
    13321 cgttgtcggc atcgaacttc gagccggtcg gcttcgtgaa caggtagacg aagcgctcgg
    13381 cagccgggtc gcgcgggtcc acgagcctga cctcctgacg ctggtcgccg gtcccggggg
    13441 ggccgggctg gctcaggtcc gcctgcacgc ccgcgtcgtc ggccatgaag acgacctcgt
    13501 cgtcggcgtc gagcgtcggg accgggtcgg tcgtcgggaa gcccttcagc ttgctcgtgt
    13561 cctcctcggg gtactcggcg aagcaccgcc cgccggtctt cttccacgac tcgacgtccc
    13621 acgcgtaggt gagttccttg tcggtgccgg agtacatgcc gaagtccgag ttcgggttcg
    13681 cgaggaagta cgggaagcgc tcgtcgacct ggaccgggat ctcgcggaag cggtccccgt
    13741 cgaaggcgta ggcgacgacg tcgtcggggt tcacgccgac ggcgggcggg acgacgagct
    13801 gcccgttgtg ggcgtcgcgc tcgccgacga ccgcgccgct ggggtagggc tcggcgacgc
    13861 cctcggcggg cgcgccggac cagcccggga gctgggcccc cgtgaggatg atcggctcga
    13921 cgctgcggtc cggggcgtcg gccggggacg cggcgaaggc cgcggtaggt acgacggcga
    13981 ggatcgaaac gctcgccact agcacgcgca gcagttgctt gttcaagaaa gtccccctgg
    14041 gtgacctagg cggaaggggt caaccggggg ttcgccgcgc tgcggccagt ctccttttcg
    14101 gggtgactag cgggtcaggc cgttcgtcga gagcacgagg tcggcggccg agtcctgcag
    14161 gtcgcgggca gcggggtgga gcccctccgg ccacacgagc acgtcgggcg cgacgtagcc
    14221 gagctgcggg tcggcagccc cgaccacgcc ctggttcgcg aagtcctcga tcctgcgcaa
    14281 gagccctcct ctcgtctgtt cgccgccggg tgttccaccc aaggtagcga acaggccctt
    14341 gacccgcagg tctagtgcgc tatttggcgg cccccggggc gttgaagaag ccctgcatct
    14401 gcatcgcgaa catcatgtcg ccctgcagct tgatcttccc cgtcatgaac agctgcggcc
    14461 cctgcgcctg gccgaagatc agccggatga agtcgaccaa cccgatctga agggtgagcc
    14521 gggggttcgt agccggccct tcagacgtcg tgcacttccc gccggcgaac gagacgctcc
    14581 acgtcttcgt cccgtcctcg gtcgagatgt cgtactggat cgtcgagtcg acgccggcgg
    14641 cccgctcggg gacgaatgcg tcctccatcc cactgaagat cgcccggagg acctcctcgg
    14701 tcccgatgcc gcggatctgc tcggcgagcg cggcgtcgac gaggtccttg atcgcttctg
    14761 ccaggactgc ggggtcgggc gtgtcggcca tggtgctcct cctgagtcgt gggcgtggtc
    14821 cgcaagacta gatgggcagg ggcctttggc gcccggaaac ccgtgcttct gtcacacccg
    14881 ccccctacgc tgggcggatg ggtgaatcac acgtttgtaa gtcgatcctc gagggcctga
    14941 accccgagca gaccgaggcc gtccgggccg tgcgagggcc ggtctgcatc ctggcggggg
    15001 ccgggtccgg caagacgacg acgatcacac gccggatcgc ctaccaggtc gccacgggga
    15061 ccttccgcgg cggcgaggtc ctcgcggtca ccttcaccga caaggccgcc ggcgagatgc
    15121 ggtcgcggct cgcggccctc ggcgccgacg gggtccgggc ccgcacgttc catgcggccg
    15181 cgctcgccca gctgcggcac ctcgccccga ccgagccccc gcggatcctc ccgtcgaagg
    15241 cggtcatgct ccgccagatc ggcaacaccc ttccgaagcc gtaccggttc cggcccgccg
    15301 gggacctcgc gaccgagatc gagtgggcga agacccgccg gctctccccg gaggactacg
    15361 cgcgcagcct cgggggccac gtcccgccga tccccgcgga cctcatgacc ggcgtctacc
    15421 gcgagtacga acgtcgcaag acacgggaag gcgcgatgga cttcgaggac ctgctcgagg
    15481 ccacgatccg gatgttcgcc gaggacgagt gggcgctcga acgcttccac cagcgctacc
    15541 gggcgttcac cgtcgacgag taccaggacg tgaacctcct ccagcagacg ctgctcgaga
    15601 cgtgggtcgg cgaccgcgac gacctctgcg tcgtcggcga cgactaccag tcgatctact
    15661 ccttcacggg agcgtcgccg cgctacctgc tcgagatgcc tcgcaagtgg ccgcgcgtca
    15721 aggtcgtgcg gctcgaggag aacttccgct cgacgccgca ggtgctgacg atggcgaacc
    15781 ggctcgtgcc gaagctcggg ggggccgaga agacgctgcg ccccgtgcgc gaggacggcc
    15841 cggcccccgt ggtcgcgccg ttcgtcgacc gggggtccga gaccaagttc ctcgtcgaca
    15901 agatcgccga gctccacgcg gccggcgtgc cctacgagga gatggccgtc ctgtaccggg
    15961 tgaacttccg ctcggaggac tacgaggacg cgctgggggc cgcgcgcatc ccgttccagg
    16021 tccgggacgg ggcgttcctc acacgcatcg ccgcgcgccg gctgctgtcg acgctgcgcc
    16081 ggtcgaaggt gaacgacgtc gcggacgaag tgctgcgtgt ggcgaagcgc gacggcttcc
    16141 aggaacgtcc gccggagggc ctgggcgacc aggagacgac gcgccagaac gacctcgcac
    16201 ggctcgtccg gctggcccgc gagttcgacg gctcgaccgc ggccgagttc gtcgcgcacg
    16261 tcgaggggcg cttcgcctcc gacggcgagg gccacggcgt gcacctgctg acgctccacc
    16321 gcgcgaaggg gctcgagttc gaagcggtgt tcctaccgcg gctcgaagag ggcgagctgc
    16381 ccggcaagcg atccaagacc gacgaagcga tcgcggagga gcggcggttg ctctacgtgg
    16441 ggctaccgcg ggcgaagcgc cacctctacc tgacctggac gaccgggggg cgccagtcgg
    16501 cgagccgttt cgtcgaggag ctcggcgggt gcctggttcc gcacccgctg cgccagacga
    16561 agcggcggcc ggaggccgcg cacgaccccg tgtaccaggc gctgaaggcg tggcggctcg
    16621 agcgcgcgaa gcgcgacggc gtccctgcct acgtgatctt ccacgacacg acgttgcagg
    16681 agatcgccga ggcgtcgccc cgatcgatcg tgcagctggc gcacatctcc ggcgtcggcc
    16741 cggccaagct cgagcgctac ggcaacgagg tgatcgtcac cctccacgag gccgtggcct
    16801 cgtaagtccg gttggggtga ggccgtcgcc acaccccatg ctgcgcccgg ctcgtagcaa
    16861 tagcgggtgc gcctggctcc cgccctcctg ctgctgttcg ctgcgtgctc gggtcatcgt
    16921 gtcgacctca cggtcgtcgc cccggcgacg cttcatctcg agccccccgg ctacgaggag
    16981 gccgtcgtgt cgcgagcggt cgacggcgac acgatcgagg tcgaggtgac cgggcgcgtc
    17041 gaggggccgg gcgcgggcga agcggtggtg ggggagacct acgtcgtacg gctcctcggc
    17101 atcgacacgc cggaatcggt gagcccgcgc gagcccgtcg agtgcttcgg gcgcgaagcc
    17161 tcggccgcta cggccgcgct cttgcagggg acgacggtga aactggtcaa ggacgtggag
    17221 gagcgtgact cgttcgaccg gctgctgcgg tacgtgtaca tgggggccga gctcgcgaac
    17281 gcaaggctgg tgacgaacgg ctacgcccac gcgtacacct accccccgaa cgtccggcac
    17341 gcacagctcc tctcggaggt cgaacgggcc gcgcgggacg ggggccgcgg gctctgggcc
    17401 cccgaggctt gtccctagtc gggcacgacc acgacgcggg cgtcggcgac gtgctcgtcc
    17461 ccggtcgccc tgagcgagta cgtccccggc gggacgtccg ggacctccac cgtcgcatcg
    17521 aacgagtact cgtcgtccgc gtcaagggtg cggaggtgga cgcttccttc gccggcggcc
    17581 cacagcccca ggcggacgtc ctcgatcgga tgccccaacg gcggagggtt gcacaccggt
    17641 cccggcgtgt cgttgcacgc gtcgccccag ctgtcgccgg tcaccgacag ctcgtcgccg
    17701 gacacgaccc ggcgcggcga gacgtggacg ctgggcgccg cgcaatccgc cgcggcttgg
    17761 gagccgagcg acagcgccgc ccccgcgagc acgagcgcca ccgtcaatcg cagcggtgcg
    17821 accttcggcc tcaagaggag cgcgctcggt agaccagcgc agtgtcgctg aaacgtccgt
    17881 aggcggtcag cgtgtcgcct tcgatctcga acgtgaagcc gtcggtcacg gtgaagaccg
    17941 cgtcgttctg cgctgtcatc gcgtcggaac actggccgtc gtctgcgctc aggctgagga
    18001 acatgatcct gtcggcctcc tcggcccact cgcccttcag accgcgacag ccggtcgacc
    18061 cctcgaactt cccgtccttg tcgaagtaaa ggaaggccgg gcgagcttcg gaggtcgccg
    18121 cgtcggggcc gcgtccgtgg acgatcgact cgagctccca ccggacgccg gtcagcttcg
    18181 cagtcggcgg gggcggcacg aactcgaaga cgagggctgc gtcgggtccc gtgagcgtga
    18241 gccgttcgcc cgaacgctcg atggtctcgg ccgccatcag cgcggcctgg tagcgggcct
    18301 ccctctcggc ggcctcgggg cggcagccca tctcggtccc gccgatgccg cccaccgaga
    18361 tcgacgttcc gtcgacggtc gccgaggccc cgtagtagtt gcaggccgag aggccgcccc
    18421 accgaccgcc gttgatcgtg agagtgatcc tgaaccgctc gctgatctcg acgggtcctt
    18481 cgggggcgta tccctccacg agctcccacg tcccgtcgta agagacgctc cgcgcggcgg
    18541 ggtcgtcgtg actagggctc gaggcggacc cggcgtccga aggcgggcct ccgcacgcgc
    18601 cgacgaggag agacgtcaga gccgccagga tccacctcat cgccgcctcc ccgtcttcct
    18661 tccgacgacc gtcgcgtccg atatcccttc gacgcgctgc cgcccaacga tgttcccacg
    18721 gccggattcg tagatcgggc ggcgtgccac tgctgtggct gagcttgggc acgccggcta
    18781 gccgggccgg tctcctcggt cgagcatgtc cacgaacttc gctatgcggc gggctcgggt
    18841 ctcggcccgt ttcgcgtcct gcaattgata gaggatcgag tagcgctgcg agctcgtgag
    18901 cttctcgaac agctccttcg cggccggctt cgcctcgagc gcggcggcta ggtcgtccgg
    18961 caccgttgcc gtagagggac tgtcgtatgc ggccgcccag cgcccgtcgg ccttggcccg
    19021 ctcgacctct gcgagccccg gcggcttcat cttgccgcgg gcgatcaggt cctcgacctt
    19081 tccgacgttg atcttcgacc acttgctccg ggcgcggcgg ggcgtgaacc gctggacgaa
    19141 gtactcgtcg tccagacgct tcatctggcc gtcgatccag ccgtagcaga gagcgacctc
    19201 gacggcctcg gcgtacgtca cggtcttctt gcgtgcgccc ttcttggcga tcttgagcca
    19261 gatgccgtcg cttttcgcgt gatcctcggc gagccatcgc tcgaaggccg cctgcgtcgg
    19321 gaactcgagt ccgtccatgg ctcgactcta gatgcgacct gcagcggctc ggcgcacc
//
LOCUS       DASQDO010000300         7687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_31183, whole genome shotgun sequence.
ACCESSION   DASQDO010000300 DASQDO010000000
VERSION     DASQDO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31183"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VEV43_08520"
     CDS             complement(<1..408)
                     /locus_tag="VEV43_08520"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HYP23604.1"
                     /translation="MEARKLRRRSHRDAQRAYLLEGTVPVREALARRAAIRHVFVSTD
                     HPEAGALATEATEAGAEAVAATPAVLEAVSDATTPQGVVAVVEMDDVALADVDEDADL
                     ILVLDQIRDPGNAGTLVRCATAAGAGAVVFTKGC"
     gene            complement(447..812)
                     /gene="rplT"
                     /locus_tag="VEV43_08525"
     CDS             complement(447..812)
                     /gene="rplT"
                     /locus_tag="VEV43_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HYP23605.1"
                     /translation="MPRVKRSVAGRKRRKKVLDQAKGYYSARGKHYRAAHEQVLHSGV
                     YAYRDRRARKGDFRRLWIIRINAAARENGLSYSRMMAGLKLAEVEVDRKMLAELAVNE
                     PATFTALADVARQSLEQAS"
     gene            complement(841..1038)
                     /gene="rpmI"
                     /locus_tag="VEV43_08530"
     CDS             complement(841..1038)
                     /gene="rpmI"
                     /locus_tag="VEV43_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HYP23606.1"
                     /translation="MPKQKSHRGAAKRFKVTSTGKIRRRRAFLNHMLEKKSPKRKRNL
                     SAPDAVVSGEDAKRIRRLLGK"
     gene            complement(1042..1692)
                     /gene="infC"
                     /locus_tag="VEV43_08535"
     CDS             complement(1042..1692)
                     /gene="infC"
                     /locus_tag="VEV43_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013138712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HYP23607.1"
                     /translation="MATDLRINDRIRVPRVRVVGADGSQIGILETKDALDMAASQDLD
                     LVEVASQADPPVCRIMDYGKFKYEQDQRQKEARKKQSQVVVKEMKMRPKIDRHDYATK
                     RGHVIRFLRQGAKVKITIMFRGREMAHQELGRRLLDQLATDVADIAKVDMPPRVDGRN
                     MTMVLNPLKEAVTAKAPAAPMKPRPPREEQPPAEEAPAPPPPARVPPRKVTRAEGG"
     gene            1899..2387
                     /locus_tag="VEV43_08540"
     CDS             1899..2387
                     /locus_tag="VEV43_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23608.1"
                     /translation="MHDDQEEQQGERPRPKVVDKRISARSAEPAPAPEPAPAGASDAT
                     ADVTVGTVPEAPASEPPRPEPGERVWTPEQEAEARRMAEEIARTPSIEWVINTAATLA
                     NVAGTKLDLGAAPDAQLAIDALAGIVNSVGTRLGGAEAPLRQLLADLQLAYAQRVTGP
                     GA"
     gene            complement(2384..2905)
                     /locus_tag="VEV43_08545"
     CDS             complement(2384..2905)
                     /locus_tag="VEV43_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23609.1"
                     /translation="MRTRSWVVLGIAVVLGVSLAAAAGVAQDVRRARGRVDAQSFAAV
                     PSSVRASPAEWRPVEGLKLRLSCPSSRVATATASAVLGAGPAARLRVEALDPGVSRPS
                     GAPDRVASPGAIPVPASDGRRAVSFTFGMERLPGSHGAVVRLAWRSPSGRALSMTKGS
                     LRVLWDRSADPCR"
     gene            complement(2972..3823)
                     /locus_tag="VEV43_08550"
     CDS             complement(2972..3823)
                     /locus_tag="VEV43_08550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYP23610.1"
                     /translation="MLLTAAVAVVGLSTVASASKGDCKAKDHDHDGVYTEGETQECGG
                     QEYGQGGHDGEPHHVPSDGSHQWGPFHWSKPSGEERKIIIRDSTTGSWATKLPNAISD
                     WGASSKFRFVKQQAATDTTTRSNCAFPDSYGRVHVCNYDYPWGDAGRADIRVNSANHI
                     QRGWAKLNNDTPDADRRSTICHELGHTLGLDHRGATSSCMYGEADAFPVNPDQHDYEQ
                     LVNQTHSHGGESTTGGINSDLDVGGGVQDGCTQFLCVDHERARNGYNIRIRWFASPID
                     ARLLRLL"
     gene            complement(3978..4505)
                     /locus_tag="VEV43_08555"
     CDS             complement(3978..4505)
                     /locus_tag="VEV43_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23611.1"
                     /translation="MTVGCLIGISMVPVPAAAHDHRPPRATLASAADQQRGRPLHSAW
                     LARADSRFCDFMTRRALPSFPRALPHATGEEVRILLPKAAAPLELSVEAWRAVGSDGR
                     PEGARETVPAALVPRLEGTQVAAWEIRFVPPPVTPHLYLAVEAYWADEESCSRPPDLG
                     SQSASWAFHLAGTGR"
     gene            complement(4570..5445)
                     /gene="hisG"
                     /locus_tag="VEV43_08560"
     CDS             complement(4570..5445)
                     /gene="hisG"
                     /locus_tag="VEV43_08560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYP23612.1"
                     /translation="MLKLVLPKGSLERATFELFEAADLKVRRGSDRDYRGTIEDPRVT
                     SVAILRPQEIPTYVEEGLFDVGVTGEDWIAETGADVVKVTALEYSKQSDRPVKVVLAA
                     PRDAGIHRPEDLPPGSKISTELPNLTKAYFERLGIPVKIFLSYGATEAKVPEIVDAIV
                     DLTETGSTLRRNGMEIVDVLLESRTQLIANAATYADPAKRKAIDELTILLRGTIDARG
                     RVLVKLNVGRDQLDAVVDALPAMRAPTVSELAETGFYAVETVVPKATINVLIPELKAL
                     GAEDIVELPITKIVP"
     gene            complement(5445..5927)
                     /gene="ribE"
                     /locus_tag="VEV43_08565"
     CDS             complement(5445..5927)
                     /gene="ribE"
                     /locus_tag="VEV43_08565"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYP23613.1"
                     /translation="MHTYEGNFEGRGLRVAIVASRFNETIVKSLVDGALDCLRRHGVS
                     DDDVSLAWVPGAFEIPVVARRFATSGDVHAVVCLGVVIKGETAHFEFVAGGAAQGISA
                     TAQETGVPVTFGVLTTETAEQAADRAGGKMGNKGFEAALAAIEMASLLQSLPKPPQGL
                     "
     gene            complement(5930..7165)
                     /locus_tag="VEV43_08570"
     CDS             complement(5930..7165)
                     /locus_tag="VEV43_08570"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HYP23614.1"
                     /translation="MPLARVEEAVEDIRAGKMVVVVDDEDRENEGDLIFAAEKATPEH
                     ISFMVRYCSGIICVPMLGERLEELNLPLMSPENSESMGTAFTISVDAREGTTTGISAA
                     DRAETIRTLIDPDKKASDLARPGHIFPLRYTPGGVLRRAGHTEASVDLARLAGLYPAG
                     VLCEVVNEDGTMARLPDLEAFAERHGLKVVSIADIIKYRRRNEKLVQRVVEARIPTAY
                     GTWSAIGYESHDGRTHVAFVKGEPAGKDDVLVRVHSECFTGDVLGSIRCDCGSQLNDA
                     MRKIDEEGQGIVVYIRGHEGRGIGLRHKLEAYALQDGGLDTVEANVELGFSPDSRDYG
                     VGAQILVDLGISTMRLLTNNPTKRAGLEGYGLEIVDRVPLESSPNPENLRYLQTKREK
                     LGHLLDTLAETIEPGESEG"
     gene            complement(7172..7342)
                     /locus_tag="VEV43_08575"
                     /pseudo
     CDS             complement(7172..7342)
                     /locus_tag="VEV43_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
     gene            complement(7355..>7687)
                     /locus_tag="VEV43_08580"
     CDS             complement(7355..>7687)
                     /locus_tag="VEV43_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HYP23615.1"
                     /translation="VAAPEIARELHKGDSVAVNGVCLTAVTTSRRRFRAEVMGETLAR
                     STFDGVRKGAAVNLELPARLSDRLGGHLVQGHVDGTARVIRAEDGDGARRLWLEAPDE
                     VLRYLVAK"
BASE COUNT         1066 a         2726 c         2601 g         1294 t
ORIGIN      
        1 gcagcccttc gtgaagacga cggcgcctgc ccccgcggcc gtcgcacagc gcacgagcgt
       61 tcccgcgttc cccggatcac ggatctggtc gaggacgagg atcagatccg cgtcctcgtc
      121 gacgtcggcc agcgccacgt cgtccatctc gacgacggcg accacgccct gcggcgtcgt
      181 cgcgtccgag accgcttcga gcacggccgg agtagccgcg acggcctcgg cccccgcctc
      241 cgtcgcctcg gtcgcgagcg cgccggcctc gggatggtcc gtcgagacga acacgtgcct
      301 gatcgcggcg cggcgcgcga gcgcttcgcg cacgggcacg gtcccctcca gcagataggc
      361 gcgctgcgcg tcgcggtgcg accggcggcg gagcttgcgg gcctccatga ccttcgggtt
      421 ctggaggctc gagatcggac ccgtcgttac gacgcctgct ccagggactg gcgggctacg
      481 tcggcgagcg ccgtgaacgt cgccggctcg ttgaccgcga gctcggcgag catcttgcgg
      541 tcgacctcga cctccgcgag cttcaggccc gccatcatcc ggctgtagct gaggccgttc
      601 tcgcgcgccg cggcgttgat gcggatgatc cagagacggc ggaagtcacc cttgcgggcg
      661 cggcggtccc gataggcata gacgcccgag tgcaggacct gctcgtgcgc ggcgcggtag
      721 tgcttgccgc gggcggagta atagcccttg gcctgatcga ggaccttctt gcgcctcttg
      781 cggccggcga cgcttctctt gacgcgtggc atgtgcccct ccttcgaaaa cgttcgtcgg
      841 ctacttgccc agcagcctgc ggatgcgctt cgcgtcctcg ccggacacga cggcatccgg
      901 ggcgctcaga ttccgcttgc gcttgggcga cttcttctcg agcatgtggt tcaggaacgc
      961 gcgccggcga cggatcttgc ccgtcgacgt gaccttgaac ctcttggccg cgccccggtg
     1021 ggacttctgc ttcggcattg ctcagcctcc ctcggctctc gttaccttcc gcggcggcac
     1081 cctcgcagga ggcggcggcg cgggtgcttc ctcggccggc ggttgctcct cgcgaggtgg
     1141 tctcggcttc atcggcgccg cgggcgcctt ggccgtaacc gcctccttca atgggttcag
     1201 gaccatcgtc atgttcctgc cgtcgacccg tggcggcatg tcgaccttgg cgatgtccgc
     1261 cacgtcggtc gcgagctggt cgagcagacg gcgccccagc tcctgatgtg ccatctcgcg
     1321 gcctcggaac atgatcgtga tcttgacctt cgctccctgt cgcaggaacc ggatcacgtg
     1381 accccgtttg gtcgcatagt cgtggcggtc gatcttcggc cgcatcttca tctctttgac
     1441 gacgacttgc gactgcttct tacgagcttc cttctgccgc tggtcctgct cgtacttgaa
     1501 cttgccgtag tccatgatgc ggcagacggg ggggtcggcc tggctcgcca cctcgactag
     1561 gtcgaggtcc tgcgaggcgg ccatgtcgag cgcatccttg gtctccagaa tcccgatctg
     1621 gctgccgtcg gcccccacca ccctgacgcg ggggacgcgg atgcggtcgt tgatcctcag
     1681 atccgttgcg atgcttctct cctttcgacg actacaagaa aaaagccgga gacgccaaga
     1741 acgacgtcac cggcgaacgg ctcaatgggc gcgccgtgcg cgcgaccctg accgtgtgcc
     1801 ttgcggcggc gacccccgcg acgtttcggc cgggaggtga gggacggtgg ggctgtccct
     1861 tcttggcgaa gctagcgggt gaagtataac gaacgcacat gcacgatgac caggaagaac
     1921 agcagggcga gcggccgcgg ccgaaggtag tcgacaagcg gatctcggcg cgttcggccg
     1981 agccggcgcc ggcgcccgag cctgcgcccg cgggcgcctc cgacgccacc gccgacgtga
     2041 cggtcgggac ggtccccgag gctccggcct ccgagccgcc gcgtcccgag cccggcgagc
     2101 gggtctggac gccggagcag gaggccgagg cgaggcggat ggccgaggag atcgcccgca
     2161 cgccgagcat cgaatgggtg atcaacacgg cggcgacgct ggcgaacgtc gcggggacca
     2221 agctggacct cggagcggcc cccgacgcgc agctggcgat cgacgcgttg gcgggcatcg
     2281 tcaactcggt agggacacgc ctcggggggg ccgaggcgcc cctgcggcag ctactggcgg
     2341 acctgcagct tgcgtacgcg cagcgggtga cggggcccgg ggcctagcga cacggatcgg
     2401 cggatcggtc ccacagcacg cgcagcgatc ccttcgtcat cgacagcgcc ctcccggaag
     2461 gagagcgcca cgccagccgc acgacggccc cgtggcttcc cggaagacgc tccatcccga
     2521 acgtgaacga cacggcgcgc ctgccgtccg acgccgggac ggggatcgct ccgggagatg
     2581 cgacgcgatc gggcgcgccc gacggcctcg acacaccggg gtcgagcgcc tcgacgcgta
     2641 gacgtgccgc aggcccggcg ccgaggaccg cgctcgcggt tgccgtcgcg accctcgagc
     2701 tcgggcacga cagtcgcagc ttcagcccct cgaccgggcg ccactcggcc gggctcgcgc
     2761 gtacggagct ggggacggcg gcgaagctct gcgcgtcgac gcggcctcgg gctcgccgca
     2821 cgtcctgggc tacgcccgcc gcggcggcaa gcgatactcc gaggacgacc gctatcccga
     2881 gcaccaccca cgatctcgta cgcatgtccc ctcccccgaa aaggaaagcg gccggggagg
     2941 cccggccgct tccgctggtc acgtcgctag gtcacagaag ccgcagtaga cgcgcgtcga
     3001 tagggctcgc aaaccatctg atccggatgt tgtagccgtt gcgtgcgcgt tcgtggtcga
     3061 cgcacaggaa ctgagtgcag ccgtcctgca cgccgcctcc gacgtcgagg tcgctgttga
     3121 tcccaccggt cgtgctctcg cctccgtggg aatgcgtctg gttcacgagc tgctcgtagt
     3181 cgtgctggtc ggggttgacc gggaaggcgt cggcctcccc gtacatgcac gagctcgtcg
     3241 cgccgcggtg gtcgagcccg agcgtatgac cgagctcgtg gcagatcgtg ctgcgccggt
     3301 ccgcgtcggg agtgtcgttg ttgagcttcg cccaccccct ctggatgtgg ttcgcggagt
     3361 tgacgcggat gtcggcgcgg cccgcgtcac cccacgggta gtcgtagttg cagacgtgga
     3421 cgcgaccgta gctgtcgggg aacgcgcagt tcgaccgcgt cgtggtgtcg gtcgcggcct
     3481 gttgcttgac gaacctgaac ttgctgctcg caccccagtc gctgatcgcg ttcgggagct
     3541 tcgtcgccca gctgcccgtc gtgctgtcgc ggatgatgat cttgcgttcc tcgccggagg
     3601 gcttgctcca gtggaaggga ccccactggt ggctgccgtc gctggggacg tggtgcggct
     3661 cgccgtcatg gccgccctgg ccgtactcct gccctccgca ctcctgcgtc tctccctcgg
     3721 tgtagacgcc gtcgtggtcg tgatccttgg ccttgcagtc gcccttcgac gccgaggcga
     3781 ccgtggacag gccgaccacg gccaccgccg ccgtcagcaa caccaagccc actccgccgc
     3841 taaaaaggcg cgtgagaacg cgcggtcgtc cgcccatggt cccctccaat cccggtgtgc
     3901 gcccaccgcg caccatcggt cgagtagacc cagtgaacta cgtgcttgat ttgccgtcta
     3961 cgcccttcgg ccgcggacta gcggccggtg ccggccagat ggaacgccca ggaggcggac
     4021 tggctgccga ggtccggcgg ccggctgcag ctctcctcgt cggcccagta cgcctcgact
     4081 gcgagataca ggtggggcgt caccgggggc ggaacgaacc tgatctccca ggccgcgacc
     4141 tgcgtgccct cgagacgagg cacgagggct gccgggacgg tctcgcgcgc cccttcggga
     4201 cgtccgtcgg atcccacggc gcgccaagct tcgacgctga gctcgagtgg agcggcggcc
     4261 ttggggagaa ggatcctgac ttcctcgccg gtcgcgtgcg ggagcgcccg ggggaacgag
     4321 ggcagggccc ggcgcgtcat gaagtcgcag aacctcgagt cggcccgggc cagccaggcg
     4381 gaatggaggg ggcgtccccg ctgctggtcc gctgccgaag cgagcgtggc gcgcggcggg
     4441 cgatggtcgt gggccgccgc cggaaccggc accatgctga tcccgatcag gcatcccact
     4501 gtcacggata cgaccttgcc catggttagg gggttcgtga cggggagcgg cgttcctgtt
     4561 gcggggacct caggggacga tcttcgttat cggcagctcg actatgtctt cggcccccag
     4621 ggccttgagc tccgggatca gcacgttgat cgtcgccttc gggaccaccg tctccaccgc
     4681 gtagaagccg gtctccgcca gctccgagac cgtcggggcc ctcatagccg gcagcgcgtc
     4741 caccaccgcg tcgagctggt cccggcccac gttcagcttc accagcacgc ggccccgcgc
     4801 gtcgatggtc cctcggagca ggatcgtcag ctcgtctatc gccttgcgct tggcgggatc
     4861 ggcgtaggtg gccgcgttgg ctatcagctg ggtgcgcgac tccaacagca cgtcgactat
     4921 ctccatgccg ttgcgcctca gcgtcgagcc cgtctcggtc aggtccacga tcgcgtcgac
     4981 tatctccggg accttcgcct cggtcgcccc gtacgacagg aagatcttca cggggatgcc
     5041 gagacgctcg aagtaggcct tcgtcaggtt cggcagctcg gtcgagatct tcgagcccgg
     5101 cggaaggtcc tccggccggt ggatgcccgc gtcacgcggc gccgcgagca cgaccttcac
     5161 cgggcggtcc gactgcttcg agtactcgag cgccgtcacc ttcacgacgt cggcccccgt
     5221 ctccgcgatc cagtcctcgc cggtcacgcc gacgtcgaac agcccctcct cgacgtacgt
     5281 cgggatctcc tgcgggcgca ggatcgcgac cgacgtgacg cgcgggtctt cgatcgtccc
     5341 gcggtagtcg cggtcgctgc cccgccgcac cttgaggtcc gctgcctcga acagctcgaa
     5401 cgtcgctcgc tccagcgatc ccttcggaag taccagcttc agcactacag gccttgtggg
     5461 ggcttcggca gcgactggag gaggctcgcc atctcgatcg cggcgagggc tgcctcgaac
     5521 cccttgttgc ccatcttgcc gccggcgcgg tccgcagcct gctcggcggt ttcggtcgtg
     5581 agcacgccga acgtcaccgg cacgccggtc tcctgcgcgg ttgcggagat gccctgagcc
     5641 gcgccgccgg cgacgaactc gaagtgcgcg gtctcgccct tgatcacgac gccgaggcat
     5701 accaccgcgt gcacgtcgcc cgaggtcgcg aagcggcgcg ccacgaccgg gatctcgaac
     5761 gcccccggga cccacgccag ggagacgtcg tcgtcgctca cgccgtggcg tcgcaggcag
     5821 tcgagcgcgc cgtcgaccag gctcttgacg atcgtctcgt tgaaacggct cgcgacgatc
     5881 gcaacgcgca ggccgcgccc ttcgaagttg ccctcgtacg tgtgcatgct catccctcgc
     5941 tttcgccggg ctcgatcgtc tccgccagcg tgtcgagcag gtggccgagc ttctcgcgct
     6001 tcgtctggag ataccggagg ttctcggggt tcgggctgct ctcgagcggc acccggtcga
     6061 cgatctcgag cccgtacccc tccagccccg ccctcttcgt cgggttgttc gtcagcaggc
     6121 gcatcgtcga gatgccgagg tcgacgagta tctgcgcgcc gacgccgtag tcacgggagt
     6181 ccggcgagaa gccgagctcg acgttcgcct cgacggtgtc caggccgccg tcctgcagcg
     6241 cgtaagcctc gagcttgtgg cgcagtccga tcccgcggcc ctcgtgcccg cggatgtaca
     6301 cgacgatgcc ctgcccctcc tcgtcgatct tgcgcatcgc gtcgttgagc tgcgagccgc
     6361 agtcgcagcg gatgctcccg agcacgtcgc cggtgaagca ctctgagtgg acgcgcacga
     6421 gcacgtcgtc cttgcccgcc ggctcgccct tgacgaacgc gacgtgcgta cggccgtcgt
     6481 gcgactcgta gccgatcgcg ctccaggtcc cgtatgccgt cgggatgcgg gcctcgacca
     6541 cacgctggac gagcttctcg ttgcggcggc ggtacttgat gatgtcggcg atcgagacga
     6601 ccttcaggcc gtggcgctcg gcgaacgcct ccaggtccgg gagccgcgcc atcgtcccgt
     6661 cctcgttcac gacctcgcag aggacgccgg ccgggtagag cccggcgagc cgcgcgaggt
     6721 cgaccgacgc ctcggtgtgg ccggccctgc ggagaacgcc gcccggcgtg taccgcagcg
     6781 ggaagatgtg gccgggccgc gccaggtcgg acgccttctt gtccgggtcg atcagggtcc
     6841 ggatcgtctc ggcccggtcg gcggcggaga tgccggtcgt cgtgccctcc cgggcgtcga
     6901 cgctgatcgt gaacgcggtg cccatcgact cggagttctc gggcgacatc agcgggaggt
     6961 tcagctcctc gagacgttcg cccagcatcg ggacgcagat gatccccgag cagtaccgga
     7021 ccatgaacga gatgtgctcc ggcgtcgcct tctccgcggc gaagatcagg tcgccttcgt
     7081 tctcgcggtc ctcgtcgtcg acgacgacca ccatcttgcc ggccctgatg tcctctacgg
     7141 cttcctctac gcgtgccagc ggcatgacgt cctacttcct ctctaggagt ctctcgacgt
     7201 acttcgccag cacgtcgact tccacgttga ccttcgatcc gaccttcagc gctccgatcg
     7261 tcgtcgcctg cagcgtgtgc gggatgatcg ccacctggaa cgacgtgcgg ccgacctcga
     7321 cgatcgtcag cgagacgccg tcgagcgcga ccgatcactt cgctacgaga taacgaagca
     7381 cctcgtcggg cgcttccagc cacagccggc gcgctccgtc gccgtcctcg gcccggatca
     7441 cgcgcgcggt cccgtcgacg tggccctgga ccaggtgccc gccgagccgg tcgctgagtc
     7501 gcgcggggag ctcgaggttc accgcggccc ccttgcgcac gccatcgaag gtcgagcgcg
     7561 cgagcgtctc gcccatgacc tcggcgcgga agcggcggcg cgaggtagtg accgcggtca
     7621 gacagacgcc gttgacggcg accgagtcgc ccttgtggag ctctctcgct atctcggggg
     7681 ccgcgac
//
LOCUS       DASQDO010000301         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_582369, whole genome shotgun sequence.
ACCESSION   DASQDO010000301 DASQDO010000000
VERSION     DASQDO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582369"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VEV43_08585"
     CDS             complement(<1..339)
                     /locus_tag="VEV43_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23616.1"
                     /translation="METAARMAGRDRRFEDLYAAHGRDAVRLGYLLTGSVADAEDLAQ
                     EAFVRLLGKFGDLRKPEVFRTYLMRTVANLAKSRYRHNEVERRHRASLATRGSVDPPD
                     VGDRDQLLSAL"
     gene            472..675
                     /locus_tag="VEV43_08590"
     CDS             472..675
                     /locus_tag="VEV43_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HYP23617.1"
                     /translation="MAVIKTIDLVGVSSESWRDAANQALAEASKTIRGITGMEVLDTS
                     CNVSDGTISEYLTHVRIKFRIER"
     gene            680..>1717
                     /locus_tag="VEV43_08595"
     CDS             680..>1717
                     /locus_tag="VEV43_08595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HYP23618.1"
                     /translation="MSGIALVGIQWGDEGKGKVTDLLAERTDVVVRYQGGNNAGHTIV
                     VEGERYALHLVPTGILYPHCTPVIGPGVVVNPQVLLEEMDNLTSRGVDTGRLLLSGNA
                     HLIMPYHLELDTVTERRLGKNRLGTTKRGIGPTYADKASRIGLRVQDLLDPKIFSEKL
                     TVALKEKNLLLTRVYGRLPMEQATIEEEYLAYAERLAPHITDTSLFVQRALDSGRAVM
                     FEGAQATMLDLDHGTYPFVTSSNPIAGGACAGAGVGPRDIERVIGIAKAYCTRVGSGP
                     FPSEADPADAEILVEAGAEYGTTTGRKRRCGWFDAVAARYAARLNTLTELVVTKLDVL
                     SEFESVKVCVAY"
BASE COUNT          280 a          590 c          581 g          266 t
ORIGIN      
        1 gagggccgac agcagctggt cgcggtcgcc gacgtcgggg gggtcgaccg agcctcgcgt
       61 cgccagagag gctctgtgcc gccgctccac ctcgttgtgc cggtaccggc tcttcgcgag
      121 gttcgcgacc gtcctcatca gataggtcct gaagacctcg ggcttgcgca ggtcgccgaa
      181 cttcccgagg agccgcacga acgcctcctg cgccaggtcc tcggcgtccg ccacgctgcc
      241 ggtgaggaga taccccaggc ggaccgcgtc gcgtccatgc gccgcgtaca ggtcttcgaa
      301 ccgcctgtcc cgtcccgcca tccgagccgc cgtctccatt gccgtccctg tcgtcgcgtt
      361 cttatacgtc catagacgcc ggcccccccg tcgaggtccc gcggctcctc gatagaaacg
      421 cccgcggctg gcaggaacca cccgtccgga accgagggac tgaggttgcc catggctgtg
      481 atcaagacga tcgacctggt cggcgtgtcc tccgaatcgt ggcgcgacgc ggcgaaccag
      541 gccctggccg aggcctcgaa gacgatccgc ggcatcaccg ggatggaagt cctcgacacg
      601 tcctgcaacg tgtccgacgg cacgatcagc gaatacctga cccacgtccg gatcaagttc
      661 cggatcgagc gttagagcag tgtccggtat cgccctcgtc ggcatccaat ggggcgacga
      721 gggcaagggg aaggtgacgg acctcctcgc ggagcgcacc gacgtcgtcg tccgctacca
      781 gggcggtaac aacgcggggc acacgatcgt cgtcgagggc gagcggtacg cgctgcatct
      841 cgtgcctacg gggatcctgt atccgcactg caccccggtg atcgggccgg gcgtcgtggt
      901 caacccgcag gtgctcctgg aagagatgga caacctgacg agccgcggcg tcgacacggg
      961 gcgcttgctg ctgtccggga acgcgcacct gatcatgccg taccacctcg agctggacac
     1021 cgtcacggaa cgacgtctcg gcaagaaccg gctcggcacc acgaagcgcg ggatcggccc
     1081 gacgtacgcg gacaaggcgt cgcggatcgg gctgcgcgtg caggacctcc tcgacccgaa
     1141 gatcttctcc gagaagctga cggtcgcgct gaaggagaag aacctcctgc tcacccgcgt
     1201 ctacgggcgg ctcccgatgg agcaggcgac gatcgaagag gagtacctcg cgtatgcgga
     1261 gcggctcgct cctcacatca cggacacgag cctgttcgtc cagcgcgctc tcgattccgg
     1321 gcgcgcggtg atgttcgagg gagcgcaggc gacgatgctc gacctcgacc acgggacgta
     1381 tccgttcgtg acgtcgtcca acccgatcgc aggcggcgcg tgcgcgggtg cgggcgtcgg
     1441 cccccgcgac atcgagcgcg tgatcggcat cgccaaggcc tactgcacgc gcgtcggctc
     1501 cggcccgttc ccgtcggagg cggacccggc cgacgcggag atcctcgtcg aggcgggggc
     1561 cgagtacggc acgacgacgg gacgcaagcg ccgttgcggt tggttcgacg ctgtcgccgc
     1621 ccgctatgcg gcgcggctca acaccttgac ggagctcgtg gtcacgaagc tcgacgtgct
     1681 ctcggagttc gagtcggtga aggtctgcgt cgcgtac
//
LOCUS       DASQDO010000302         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_643702, whole genome shotgun sequence.
ACCESSION   DASQDO010000302 DASQDO010000000
VERSION     DASQDO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643702"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="VEV43_08600"
     CDS             <1..1451
                     /locus_tag="VEV43_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYP23619.1"
                     /translation="QVEDGANVVEYLGKNAPWSNGRVGMYGASYDAETQISTAAFGDP
                     SKVKYLKAIVPTASVGGQYDWNFMDGVPWFGQPLIGNSSYLATTSLTPGQMPAPQHYP
                     EKLECQGEVMASSADQNGDFTEYWQEREYRPKAHKVKAATLMVHGLRDFNVQDITLAG
                     FFDRLSDKTPHKGLFGVWNHAFPSAHSAVEPEWARADWYPMVTAWFDRYLKGLDTKVE
                     RWPDVQVQASSGEWWSVPEYPTTGGPLGQLALGADGSLGTKSPKGESLFTEQVAPGPP
                     EDGQSVVFETPKLKKSLHVTGQPMLDLWLMSTRPDGHVGAELEVVGPDGEPLQHDGSY
                     SEYQATYGVRSLQHLDPMPNGWFQQEAAKEIATNEPVRVTIRLLPTDLVVPKGGSLRL
                     TIAGGVSYSKGDSLPSGAASEITILHNCERPSFLRFRMPDPKARLLNVREHDEAKLPK
                     LATSNARVGRRDGGGLATSRVCGLRPRALPFL"
     gene            complement(1473..2717)
                     /locus_tag="VEV43_08605"
     CDS             complement(1473..2717)
                     /locus_tag="VEV43_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP23620.1"
                     /translation="MRVRPLAAALLLLGGLPAGAAPAARGVSRPVATVIAVVEPGGLN
                     VLHAEFRAQRGATLPAGVPDDVEPIRLPRGGDFEERMRRASAGPLGRLEPGQLYHLAG
                     TRVLLYPSPGRGLQNVFDAPEHGTGTASSAAGRRSGTDPHAWLVYVLGGDGASWRWLA
                     EQSWIDVVSSSYYAVVPNGNGLCPEVTHIRTISDEGRLVFTAAGNQEQAGFAHSPAGA
                     PGAYQVGGVDESGRTYLRPDAAPTEPDWATATPTRPYETGDRFRYEAADPGSVDGAVT
                     FGGTSGAAPSTAGRATTLIRYARRVLGSSGTGVRRGALAEAPKGRDVPARGPLADGDL
                     TAAELTSLLHHVAVPAEPPSPARYMVEGYGALGDEAMARAVDVLRGAESEPERPAEDS
                     AHDIVETAREALFADAVRRCGS"
     gene            complement(2723..3187)
                     /locus_tag="VEV43_08610"
     CDS             complement(2723..3187)
                     /locus_tag="VEV43_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23621.1"
                     /translation="MQRISWRLAATATAAALATTGALAPASASRGARTTRVETATYDA
                     PGGVGALSVSVTGTNVGAVMLPAGPERKVSVEVTDATGLPVSAEIKQRGGDGVALESN
                     DTAVPFCGATEKPVTVRPNEPVYVFLYAGPCEDGTPAAATTGEVVATFVGRG"
     gene            complement(3332..3538)
                     /locus_tag="VEV43_08615"
     CDS             complement(3332..3538)
                     /locus_tag="VEV43_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23622.1"
                     /translation="MRKALVVLSIVGAMMLPMGAAHADDKPQIGGFGVPVPGTGCTVY
                     TPYVFVGSVPGGPFYVGPGYVSCP"
BASE COUNT          544 a         1269 c         1256 g          508 t
ORIGIN      
        1 accaggtcga agacggcgcg aacgtcgtcg agtacctcgg gaaaaatgcc ccgtggtcga
       61 acggcagggt cggcatgtac ggcgcgtcct acgacgccga gacgcagatc tcgaccgctg
      121 cgttcggcga cccctcgaag gtgaagtacc tgaaggcgat cgtcccgacc gcctcggtgg
      181 gcgggcaata cgactggaac ttcatggacg gcgtgccgtg gttcggccag ccgttgatcg
      241 gcaactcgag ctacctcgcg acgaccagcc tcaccccggg gcagatgccg gccccccagc
      301 actaccccga gaagctcgag tgccagggcg aggtcatggc ctcgtcggcg gaccagaacg
      361 gtgacttcac cgagtactgg caggagcgtg agtaccggcc gaaggcccac aaggtgaagg
      421 cggcgactct gatggtccac ggcctgcgcg acttcaacgt ccaggacatc acgctcgccg
      481 gcttcttcga ccggttgtcg gacaagaccc cgcacaaggg cctgttcggc gtctggaacc
      541 acgcgttccc gtcggcgcac tcggccgtcg agcccgagtg ggcacgcgcc gactggtacc
      601 cgatggtcac ggcgtggttc gaccgctacc tcaagggtct cgacacgaag gtcgagcggt
      661 ggccggacgt ccaggtccag gccagcagcg gcgagtggtg gagcgtcccc gagtacccga
      721 cgaccggggg ccccctcggt cagctcgcgc tgggcgcgga cggatccctc ggcacgaagt
      781 cgccgaaggg cgagagcctc ttcaccgagc aggtcgctcc cggcccccct gaggacggcc
      841 agtccgtcgt cttcgagacg ccgaagctga agaagtcgct ccacgtcacg gggcagccga
      901 tgctcgacct gtggttgatg tcgactcgtc cggacggcca cgtgggggcc gagctcgagg
      961 tcgtcggccc ggacggcgag ccgctccagc acgacgggag ctactccgag taccaggcga
     1021 cctacggcgt ccgttcgctc cagcacctcg acccgatgcc gaacggctgg ttccagcagg
     1081 aagccgccaa ggagatcgcc acgaacgagc ccgtccgtgt gacgatccgc ctcctcccga
     1141 ccgacctcgt cgtcccgaag ggcgggtcgc tccggctcac gatcgccggc ggcgtctcgt
     1201 actcgaaggg tgactcgctg ccctcggggg ccgcgtcgga gatcacgatc ctgcacaact
     1261 gcgaacgccc gagcttcctg cgcttccgca tgcccgaccc gaaggcccgc ctgctgaacg
     1321 tgcgcgagca cgacgaggcg aagctgccga agctggcgac gtcgaacgcg cgggtgggcc
     1381 ggcgcgacgg cgggggcctg gcgacgtcga gggtctgcgg cctgcggccc cgcgctctgc
     1441 cgttcctgta agaagagtcc ggtggtaggc ctctagctgc cgcaacgcct gacggcgtcg
     1501 gcaaagaggg cttcccgcgc cgtctcgacg atgtcgtgcg ccgagtcctc ggccgggcgc
     1561 tccggctccg actcggctcc ccgaagcacg tccaccgccc gagccatggc ctcgtcgccg
     1621 agagcgccgt agccctcgac catgtagcgg gcgggcgaag ggggctctgc gggaacggcc
     1681 acgtggtgga gcaatgacgt gagctcggcc gccgtcaggt cgccgtccgc gagcgggcca
     1741 cgggccggga cgtcgcggcc cttcggcgcc tcggcgagag ctccacgccg aacgcccgtc
     1801 cccgacgatc ccaggacgcg tcgcgcgtaa cggatcaacg tcgtggcgcg ccccgcggtg
     1861 ctgggcgcgg cccccgacgt gcccccgaac gtgacggccc cgtcgaccga gccggggtcg
     1921 gccgcctcgt acctgaaccg gtcaccggtc tcgtaaggcc gagtcggcgt ggctgttgcc
     1981 cagtccggct cggtcggggc agcatccggg cggaggtacg tgcggcccga ctcgtccacg
     2041 cctcccacct gataggcgcc cggtgcgccg gccggtgaat gcgcgaaacc ggcctgctcc
     2101 tggttccccg ctgcggtgaa cacgaggcgc ccctcgtcgg agatcgtccg gatgtgcgtc
     2161 acctccggac agagcccgtt gccgttcggg accacggcgt aatagctgct ggagacgacg
     2221 tcgatccacg actgctccgc gagccaccgc catgacgcgc catcgccgcc gagcacgtag
     2281 acgagccagg catgagggtc cgtgccggaa cgccggccgg cggcggagct ggcggtcccg
     2341 gtgccgtgct cgggcgcgtc gaagacgttc tgcaaacccc gtccgggcga cgggtacagc
     2401 aagacccgcg tgccggcgag gtgatagagc tgcccgggtt cgaggcgccc gaggggacct
     2461 gcggacgccc gtctcatgcg ctcttcgaag tcgcccccgc gcggcagccg gatcggttcg
     2521 acgtcgtccg ggacgcccgc gggcagcgtg gcgccgcgct gggctcggaa ctccgcgtgg
     2581 aggacgttga ggccgccggg ctctacgact gcgatgacgg tcgcgaccgg acgactgacg
     2641 ccacgtgcgg ccggcgccgc gcccgccggg agtccgccga ggagcaagag ggccgcggcc
     2701 agagggcgga cccgcatggt cgtcagcccc ggccgacgaa ggtggcgacc acttcgcccg
     2761 tcgtcgccgc ggcgggtgtc ccgtcctcgc agggacccgc gtacaagaag acgtatacgg
     2821 gttcgttcgg ccgcaccgtc acgggcttct ccgtcgcgcc gcagaacggg accgccgtgt
     2881 cgttgctctc gagcgcgacc ccgtcacctc cgcgctgctt gatctcggcc gagaccggaa
     2941 ggccggtcgc atccgtcacc tctacggaga cctttcgctc gggcccggcg ggcagcatca
     3001 ccgcgcccac gttcgtgccc gtcaccgaga ccgagagagc gcctactcca ccgggagcgt
     3061 cgtacgtggc cgtttcgacc ctcgtggtcc gggcgcctcg gctcgcggat gcgggggcaa
     3121 gggctccggt cgtcgccagc gcggcggcgg tagccgtagc ggcgagcctc cacgagatgc
     3181 gttgcatgta actcacctct cttcggtgtg acagcctcgg gaatccgttc ggcgtcgcga
     3241 cagaggttcc tgcgacggcc cctcgccgag atgacgaagg gcatgaggcg agtccgccgg
     3301 ggatcgcctc atgcccttcg gtgctgtctc gctaggggca ggagacgtaa ccggggccga
     3361 cgtagaacgg cccgccgggg acgctcccca cgaagacgta gggggtgtag acggtgcagc
     3421 ccgtcccggg gaccggaaca ccgaatccgc cgatctgcgg cttgtcgtcc gcgtgtgcgg
     3481 cgcccatcgg gagcatcatc gctccgacga tcgagagaac cacaagcgcc ttgcgcatgc
     3541 gcgtatcacc tccttccgct gagtcgagtt gatgtgc
//
LOCUS       DASQDO010000303         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_827269, whole genome shotgun sequence.
ACCESSION   DASQDO010000303 DASQDO010000000
VERSION     DASQDO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827269"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..928
                     /gene="rsmA"
                     /locus_tag="VEV43_08620"
     CDS             107..928
                     /gene="rsmA"
                     /locus_tag="VEV43_08620"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYP23623.1"
                     /translation="MSDLLGARRLRELLDAHGVRPSKSLGQNFVVDPTTIRKVVAVSG
                     VGRDDEVLEIGAGAGSLTLGLAAAAGRVVAVEFDRALHPVLAETLSGVSNVEVVHADA
                     TKLDWSSVEATALVANLPYNIATPLVLDALSSARSLRTMTVMTQREAGERLAAPPGSK
                     TYGHVSVVAAFFATATVAAPISRRVFYPEPNVDSVLVRLVRHETPRSDPSRLFALTRA
                     AFSQRRKTLRSSLAGAVPDPERLPDALRAVGVAPTARPEELGIDEWLALEGQLFA"
     gene            complement(899..1387)
                     /locus_tag="VEV43_08625"
     CDS             complement(899..1387)
                     /locus_tag="VEV43_08625"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HYP23624.1"
                     /translation="MNTGADLFKHELKDLYDAEYRQAQALTEMAEKVADGKLAEALRE
                     HREETQGQIKRLESVFEAIGEKPEKETCAGVIGLIKEFKDFTGEDPGEKVLNVFTAEA
                     ALKAEHYEIVSYSGLIKLAGQMGKTEVIDLLQQNLDEELATAQNLEILSGQLGEELAL
                     QS"
     gene            1468..>2088
                     /gene="ispE"
                     /locus_tag="VEV43_08630"
     CDS             1468..>2088
                     /gene="ispE"
                     /locus_tag="VEV43_08630"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYP23625.1"
                     /translation="MSAEQAPPHVRIKTHAKLNLFLRVLGRRSDGYHEVETILHGIEM
                     GDDIDVRLTDTGKVEVEMVLADLVSDLLPASEQNTAWHAAHYLIEHGARNEGVHIEIV
                     KRIPIGAGLGGGSGNAAGVLVALNELWKTELNRDGLLGVAGRVGSDVPYCISGGTALA
                     TARGESLTPLPSPATMWFVLGISHSPLLTRDVYGAWEPEHANDAIGS"
BASE COUNT          280 a          742 c          720 g          346 t
ORIGIN      
        1 cccgcgcacg tggcgatcgt cgggaccgcc gtcgccgaag cgcgggcgga atccgtcgag
       61 gcggtcgccg aggcgacgac gcgcaacgcg cagcgcctgc tcggccttgt ctgacctcct
      121 cggcgcgcgc cggctgcgcg agctgctcga cgcgcacggt gtccggccct cgaagtcgct
      181 cggccagaac ttcgtcgtcg accccaccac gatcaggaag gtcgtcgcgg tgtcgggggt
      241 cggccgggac gacgaggtgc tcgagatcgg tgcgggggcc gggtcgctca cgctcgggct
      301 cgcggccgca gccggccggg tcgtcgcggt cgagttcgac cgtgcgctcc accccgtcct
      361 ggcggagacg ctgagcggcg tgtcgaacgt cgaggtcgtc cacgccgacg cgacgaagct
      421 cgactggtcc tcggtcgaag ccacagcgct ggtcgcgaac ctcccgtaca acatcgcgac
      481 tccgctggtg ctcgacgcgc tctcgtccgc ccgctcgctg cgcacgatga cggtgatgac
      541 acagcgtgag gccggcgagc gcctcgcggc tccgccgggg tcgaagacgt acgggcacgt
      601 ctcggtcgtc gccgcgttct tcgcgacggc gacggtcgcg gcccccatct cgcgcagggt
      661 cttctacccg gagccgaacg tggactcggt cctggtccgg ctggtgcggc acgagacgcc
      721 gcgctccgac ccgagccgtc tgttcgcgct gacgcgcgcg gccttctcgc agcggcgcaa
      781 gacgctgcgg agctcgctgg cgggcgcggt ccccgatccc gaacggcttc cggacgcgct
      841 acgggcggtg ggagtcgcac ccaccgcccg tccggaagag ctcggtatag acgagtggct
      901 agctctggag ggccagctct tcgcctagct gcccggagag gatctcgagg ttctgcgcgg
      961 tcgcgagctc ctcgtccagg ttctgctgca ggaggtcgat gacctcggtc ttgcccatct
     1021 ggccggcgag cttgatcagc ccggagtacg acacgatctc gtagtgctcg gccttcaggg
     1081 cggcctcggc ggtgaagacg ttcagcacct tctcgcccgg gtcctcgccg gtgaagtcct
     1141 tgaactcctt gatgaggccg atcacgcccg cgcaggtctc cttctcgggc ttctcgccga
     1201 tcgcctcgaa gacgctctcg agcctcttga tctgcccctg ggtctcctcg cggtgctcgc
     1261 gcagggcttc cgcgagcttg ccgtcggcca ctttctcggc catctcggta agggcctggg
     1321 cctggcggta ctcggcgtcg tacaggtcct tcagctcgtg cttgaaaagg tccgctccgg
     1381 tgttcatggg gctccctcca taggtggacg tggtctctcc tcccgtaccc aggggcgggc
     1441 ctccgtgaac cggttacgtt gtgcgcgatg tctgctgagc aagcaccccc acacgtccgc
     1501 atcaagacgc acgcgaagct gaacctgttc ctgcgcgtgc tcggccgccg gtccgacggc
     1561 tatcacgagg tcgagacgat cctgcacggc atcgagatgg gcgacgacat cgacgtccgc
     1621 ctcaccgaca ccggcaaggt cgaggtcgag atggtcctcg ccgacctggt ctcggacctg
     1681 ctgcccgctt ccgagcagaa caccgcgtgg catgcggccc actacctcat cgagcacggc
     1741 gcgcgcaacg agggcgttca catcgagatc gtcaagcgga tcccgatcgg tgcgggcctc
     1801 gggggcggga gcggcaatgc cgcgggcgtg ctcgtcgcgt tgaacgagct gtggaagacc
     1861 gagctcaacc gcgacggcct gctcggcgtc gcggggcgcg tgggctcgga cgtcccgtac
     1921 tgcatctcgg gcgggaccgc gctcgcgacc gcccgcgggg agagcctcac gccgctgccc
     1981 tcgccggcca cgatgtggtt cgtgctcggg atctcgcact ccccgctgct cacccgcgac
     2041 gtctacggcg cgtgggagcc ggagcacgcc aacgacgcga tcggcagc
//
LOCUS       DASQDO010000304         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1194578, whole genome shotgun sequence.
ACCESSION   DASQDO010000304 DASQDO010000000
VERSION     DASQDO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194578"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VEV43_08635"
     CDS             <1..381
                     /locus_tag="VEV43_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYP23626.1"
                     /translation="LGIIPGAGGTQRLPRLEGAGRAKELIYSGRFVESSEALSIGLAN
                     EVVPAEEVYSRSVERAEAFAAGPTVALMAAKQAIQGGLQADIATGLMLERQGFAALFA
                     SEDQKTGMESFVEHGPGKAKFVGR"
     gene            390..737
                     /locus_tag="VEV43_08640"
     CDS             390..737
                     /locus_tag="VEV43_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP23627.1"
                     /translation="MASSVFCVTIDCADPKGLASFWEQVLDYRRTYDGDDEVAIAPRE
                     GRGDTLLFLEVPDEKKVKNRVHLDLNPTDQAAEVERVKALGATEVDIGQHDVTWVVLA
                     DPEGNEFCILTPR"
     gene            746..1330
                     /locus_tag="VEV43_08645"
     CDS             746..1330
                     /locus_tag="VEV43_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP23628.1"
                     /translation="MLVHGAGSGPWVFESWTHPSLDVVAVDLQEGLDPASASMDDYAE
                     VVAGPCRDAAPPVGLCGWSMGGLVAMTAAEPAGVEALVVLEPSPPAEVQGTTDAPDAT
                     GTFDPEEVYGSFPAGVRSRAESSRARADRKRGISVPRLPLRTLVVYGDEFEQDRGRDL
                     ARFYGVEEAHFPGLTHWDLVLDPRVRERVFAFVS"
     gene            complement(1386..>2406)
                     /locus_tag="VEV43_08650"
     CDS             complement(1386..>2406)
                     /locus_tag="VEV43_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23629.1"
                     /translation="GHSAGLLPEDVSAQDWLDDNWYSQNGPYSPVVQDDEAGEIIDNF
                     ISIDTPSDPACGNTGEAACEYNGNEFVYSGSIFGQPPPPDGQEPPESMTFATTIDAQQ
                     GTRIWVVNLVFPGERMKIEVVGNNEPATTQAEIDTTRAQQLAHDQEWADAMHAKMKKK
                     RTSHVAQDGTRVWDAWAGVDNEHASLNQFYPRKLKVKKGQRVRWHFSQNINQIHTVSM
                     PIPAVFNQDFEFFGTFECDSPNAPDAPAPPPASDQEEPECPAGTTLEFEFGDFLTGAG
                     NGTWSGNRDVEHSGFRGADLQHFTPPLQGKSPYTVRMGKRTGKQPMSYLCFIHESMFG
                     TVSVQ"
BASE COUNT          348 a          786 c          877 g          395 t
ORIGIN      
        1 ctgggcatca ttccgggcgc cggcggcacg cagaggctgc cccggctcga gggagcaggc
       61 cgcgcgaagg agctcatcta ctcgggcagg ttcgtcgagt cgagcgaggc cctgtcgatc
      121 gggcttgcga acgaggtggt cccggcagag gaggtgtact cgcgctcggt cgagcgggcc
      181 gaggcgttcg cagcgggccc cacggtcgcg ttgatggccg cgaagcaggc gatccagggc
      241 gggctccagg cagacatagc gacggggctg atgctcgagc gccaggggtt cgcggcgctg
      301 ttcgcgagcg aggaccagaa gaccggcatg gagtcgttcg tcgagcacgg cccgggcaag
      361 gcgaagttcg tcggacggta ggaggaaaga tggcaagcag cgtcttctgc gtgacgatcg
      421 actgtgcgga cccgaagggg ctcgcgtcgt tctgggagca ggtgctcgac taccgccgga
      481 cgtacgacgg ggacgacgag gtggcgatcg cgccgcgcga gggccgggga gacaccctgc
      541 tgttcctcga ggtccccgac gagaagaagg tgaagaaccg cgtccacctc gacctgaacc
      601 cgaccgacca ggcagccgag gtcgaacgcg tcaaggcgct cggcgcgacc gaggtggaca
      661 tcggtcagca cgacgtcacg tgggtcgtgc tggcggatcc cgagggcaac gagttctgca
      721 tcctgacccc gcgctgagca cgctcgtgct cgtccacggc gccggcagcg gcccgtgggt
      781 gttcgagtcg tggacgcacc cgtccctcga cgtcgtcgcg gtcgacctcc aggaaggcct
      841 cgacccggcg agcgcgtcga tggacgacta cgccgaagtg gtggccgggc cgtgccggga
      901 cgcagcgcca cccgtcggac tgtgtggctg gagcatgggc gggctggttg cgatgacggc
      961 cgccgaaccg gccggggttg aggccctggt agtgctcgag ccgagcccac ccgcggaggt
     1021 gcaggggacg accgacgctc ccgatgcgac cgggaccttc gatcccgagg aggtctacgg
     1081 cagcttcccg gccggcgtgc gatcgcgcgc cgagtcgtcg agggcgcggg ccgaccgcaa
     1141 gcgcggcatc tcggtcccgc ggctcccgct ccgcacgctc gtcgtctacg gggacgagtt
     1201 cgagcaagac aggggccgcg atctggcgcg cttctacggc gtcgaggaag cccacttccc
     1261 ggggctcacc cattgggacc tcgtcctcga cccacgcgtc cgcgagcgcg tgttcgcgtt
     1321 cgtctcctga cgcggaaagg cggcggcccc ggaggaccgc cgcccgcgtt ggctccgcgt
     1381 gctcgctact gcaccgagac ggtgccgaac atgctctcgt ggatgaagca cagatacgac
     1441 atcggctgct tgcccgtcct cttgcccatc ctcacggtgt agggcgactt gccctggagc
     1501 ggcggggtga agtgctggag gtccgcaccg cggaatcccg agtgctcgac gtcacggttg
     1561 ccggaccatg tgccgttgcc ggctcccgtc aggaagtcgc cgaactcgaa ctcgagcgtc
     1621 gttccggcgg ggcactccgg ctcctcctgg tcggacgccg gcgggggggc cggcgcgtcg
     1681 ggggcgttcg gcgagtcgca ctcgaacgtc ccgaagaact cgaagtcctg gttgaacacc
     1741 gccgggatcg gcatcgagac cgtgtggatc tggttgatgt tctgcgagaa gtgccaccgc
     1801 acacgctggc ccttcttcac cttcagcttg cgcgggtaga actggttcag cgacgcgtgc
     1861 tcgttgtcga caccggccca cgcgtcccag acgcgcgtcc cgtcctgcgc gacgtggctg
     1921 gtccgcttct tcttcatctt ggcgtgcatc gcgtccgccc attcctggtc gtgcgcgagc
     1981 tgctgcgcgc gggtggtgtc gatctcggcc tgtgtcgtcg cgggctcgtt gttgccgacg
     2041 acctcgatct tcatgcgctc gccggggaag acgaggttga cgacccagat gcgcgtgccc
     2101 tgctgcgcgt cgatcgtggt agcgaacgtc atcgactcgg gcggctcttg accgtcgggg
     2161 ggcgggggct ggccgaagat gctcccggag tagacgaact cgttgccgtt gtactcgcac
     2221 gcagcctcac ccgtgttgcc gcacgcgggg tcgctcggcg tgtcgatcga gatgaagttg
     2281 tcgatgatct cgccggcctc gtcgtcctgg acgacggggg agtacggccc gttctggctg
     2341 taccagttgt cgtcgagcca gtcctgcgcg ctgacgtcct ccggcagcaa gcccgccgag
     2401 tggccg
//
LOCUS       DASQDO010000305         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_93457, whole genome shotgun sequence.
ACCESSION   DASQDO010000305 DASQDO010000000
VERSION     DASQDO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93457"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VEV43_08655"
     CDS             complement(<1..280)
                     /locus_tag="VEV43_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYP23630.1"
                     /translation="MEFRRIGRFPPYVFSIVNDLKTQARRSGEDIIDLGMGNPDIPTP
                     DAVVDKLKEAAENARNHRYSASKGIPKLRRAICDLYERSWGGELDPATE"
     gene            complement(304..933)
                     /locus_tag="VEV43_08660"
     CDS             complement(304..933)
                     /locus_tag="VEV43_08660"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYP23631.1"
                     /translation="MTTLFLVRHGATKWNHSKRAQGQADIDLNETGHRQAIATAEELS
                     HVPVVAVYASDLMRAYKTAEPIARTHGLEVRTDPAFREIDQGEWEGLHVDEIRRRWPD
                     LWGPARHWSARPGGESPAQVRERALKGLEKVVREHPEGNVVVVSHGGTIRWVSAEALG
                     YDDRRSARIRGLGNGAVVRVTASIEGGDLMLRDLERLDGNTTDLDDPND"
     gene            complement(930..1799)
                     /locus_tag="VEV43_08665"
     CDS             complement(930..1799)
                     /locus_tag="VEV43_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HYP23632.1"
                     /translation="MTATFLLDAPSLVYRAYFALPTTLTDAQGRPVNAVRGFLEMVTR
                     ILIDHRPERLVSVFDGNWRPDFRVAAYPGYKAARPEDPPDLPRQFDVLEAVLDAAGIS
                     RAVSYELEADDAIATLVERLEPGERAIVVTGDRDLLALVEDPAVRVLFPVRGTKEMTE
                     FDEAAVVAKYKVAPKLYPVFATLRGDASDGLPGVAGIGPKRAADLLERFGSIDGIVAA
                     VDELPPRQASALKEANDYLEAMKTVVTLVRDADVETTPTGDPDEETLRKLGAEHNLGS
                     STVRLLQALRGER"
     gene            complement(1927..2814)
                     /locus_tag="VEV43_08670"
     CDS             complement(1927..2814)
                     /locus_tag="VEV43_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYP23633.1"
                     /translation="MRDVRLGVTLPQFTDDASVFTDGVRCAEGLGFDSLWVFDHLWPL
                     GAKRRPILEGWTALAWVAARTKEATVGTLVTRSSLRHPAVLAKMAATVSEIAPGRLCV
                     AIGSGDQASAAENLAFGLPYWTGADRMDQLASTVEVLHRSRDGEVTFADDFVALDGLP
                     AAPGPRDYSIWVAGRSVDALDITGRLADGWNAWGASPEEFVNAADALGKIAGDRLLEL
                     TWGGQVIAGATDAEAEELLGSRNPASYVVGGPDKVGRYLRSLVDAGVHHLILATPFAS
                     DPKSYELLAGPVRDAAGLA"
     gene            complement(2811..3596)
                     /locus_tag="VEV43_08675"
     CDS             complement(2811..3596)
                     /locus_tag="VEV43_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23634.1"
                     /translation="MSGAAGVVALIMLEWTVGLVGAAAWTQSWGVVRRGHFRITAYTG
                     VALAILAVLAMRSGADPVSGGAAVWIVATAAGVGLYLLVQWSNTDVPAVVVGVAATVA
                     GVGALGAAASLLEDWPVILAAVEVVAGAALLGAVTNGMLLGHWYLNQPGLKPWALARL
                     TTLSLVATIATGVLGLVAAGKLAGAETDGAVLGIPGFGESFGLAFFLVWIALVGFTGA
                     VVWAARRCVAIKSIQSATGLYYVALLTAGVSEFLVRYLMVSAS"
     gene            complement(3593..3979)
                     /locus_tag="VEV43_08680"
     CDS             complement(3593..3979)
                     /locus_tag="VEV43_08680"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HYP23635.1"
                     /translation="MSTPPVKPPPPPTSLKEYVLLVPRLGRLVWRLSRDPRVPPRIKA
                     ATFVLAGYLLSPIDFIPEFVPGLGHLDDLVILAFALDQILNRVPDEVVLDHWDGDEDV
                     LEVVRQILDISTAYMPGWLKKRFSSS"
     gene            complement(3976..>4804)
                     /locus_tag="VEV43_08685"
     CDS             complement(3976..>4804)
                     /locus_tag="VEV43_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYP23636.1"
                     /translation="VAVLEDLLPRLEGSGNEVRAIGVGAAGFVDFAAGAVTFSPNVVY
                     DDPQIATAVALRFGLPVFVDNDANAALWGERTFGCCRVAEHAVMLTLGTGIGSGIVAN
                     GELVRGATGTGAELGHIVVDPSGPPCPCGLRGCLEQLASGTAIARLAHIALGSNPDST
                     IPAFAGPGGEITGESVSKAAREYDQTARDVLRKAGTALGIGLSNVANVFDPEVIVLGG
                     SVVNAGEPYLGAARDQLALMTKAQRRRAMRLDVAALRGDAGLVGAAALAWTETVDAA"
BASE COUNT          716 a         1739 c         1681 g          668 t
ORIGIN      
        1 cctcggtggc ggggtcgagc tcgccccccc acgaccgttc gtagaggtcg cagatcgcgc
       61 gccggagctt cgggatcccc ttcgatgccg agtacctgtg gttgcgcgcg ttctcggccg
      121 cttccttgag cttgtccacg acggcgtcgg gcgtggggat gtcggggttg cccatcccga
      181 ggtcgatgat gtcctcgccc gaccgccgcg cctgcgtctt gagatcgttg acgatcgaga
      241 acacgtacgg gggaaagcgt ccgatacgcc tgaattccat gcccccgaac ctacaagagc
      301 ccgctagtcg ttcgggtcgt cgaggtcggt cgtgttgccg tcgagccgtt cgaggtcgcg
      361 cagcatcagg tccccgccct cgatcgaagc ggtcacccgc accaccgcgc cgttgccgag
      421 gccccggata cgcgcggacc tgcgatcgtc gtagccgagc gcctcggcgc tcacccaccg
      481 gatggtcccc ccgtggctca cgacgacgac gttgccttcg ggatgctcgc gcacgacctt
      541 ctccagcccc ttcagcgcgc gctcgcgcac ctgcgccgga gactcgccgc cgggccgcgc
      601 gctccagtga cgggcgggcc cccacaggtc cggccaccgc cgccggatct cgtcgacgtg
      661 cagcccctcc cactcgccct ggtcgatctc gcggaacgcg gggtcggtcc ggacctcgag
      721 gccgtgcgtg cgggcgatcg gctcggcggt cttgtaagcg cgcatgaggt cgctcgcgta
      781 gacggccacg acggggacgt gcgagagctc ctcggcggtg gcgatcgcct gacggtgccc
      841 ggtctcgttg aggtctatgt cggcctgtcc ctgcgcccgc ttggagtggt tccacttggt
      901 agcgccgtgc cgcacgagga agagcgtcgt catcgctcgc cccgcagggc ctggagcagt
      961 cgcaccgtcg agctcccgag gttgtgctcg gcccccagct tgcgcagggt ctcctcgtcg
     1021 ggatcgccgg tgggcgtcgt ctcgacgtcc gcgtcgcgca cgagcgtcac gacggtcttc
     1081 atcgcttcca gatagtcgtt cgcctctttc agggcactcg cctgacgcgg ggggagctcg
     1141 tccacggcgg cgacgatccc gtcgatggag ccgaagcgct cgagcaggtc cgcggccctc
     1201 ttcggcccga tccccgctac gcccgggagc ccgtccgacg cgtcgccccg aagcgtcgcg
     1261 aagacgggat acagcttcgg cgcgaccttg tacttggcga cgaccgcggc ctcgtcgaac
     1321 tcggtcatct ccttggtccc gcgcaccggg aacagcacgc gcaccgcagg gtcctcgacc
     1381 aacgcgagga ggtcgcgatc gcccgtcacc acgatcgcgc gctcgcccgg ctcgagccgt
     1441 tcgaccaggg tcgctatcgc gtcgtcggcc tcgagctcgt acgagaccgc ccggctgatc
     1501 cccgcggcgt cgaggaccgc ctcgagcacg tcgaactggc gagggaggtc cggcgggtcc
     1561 tcgggccgcg cggccttgta gccgggatag gccgccaccc ggaagtcggg gcgccagttc
     1621 ccgtcgaaca cggacacgag ccgttccggc cggtggtcga tgaggatgcg ggtgaccatc
     1681 tcgaggaagc cgcgcacggc gttgaccggc cgtccctgcg cgtcggtcaa ggtcgtggga
     1741 agcgcgaagt acgcgcgata gacgaggctc ggcgcgtcga gcaggaacgt ggcggtcatg
     1801 cggcgaccct atcggtcacc tacgacgcga accccgtggt tcgtgtggtg cgcgtggttg
     1861 gcgaggttgc actcccaacc accgcgacca cgcttaccac gcgaaccacg gcctccggaa
     1921 gaacacctac gcgaggcctg cggcgtcccg gaccggtccc gccagcagct cgtacgactt
     1981 cgggtccgag gcgaacggcg tcgccaggat caggtggtgg acgcccgcgt cgaccaggga
     2041 ccgcaggtag cggccgacct tgtccgggcc cccgacgacg tagctcgcgg ggttccgcga
     2101 cccgagaagc tcctctgcct cggcgtcggt ggcaccggcg atcacctggc cgccccacgt
     2161 gagctcgagc agcctgtcgc ccgcgatctt cccgagggcg tcggcggcgt tgacgaactc
     2221 ctcgggagac gccccccacg cgttccatcc gtcggcgagg cgtccggtga tgtcgagagc
     2281 gtcgaccgaa cgcccggcta cccagatcga gtagtcgcgg gggccggggg ccgcgggcag
     2341 gccgtccaga gcgacgaagt cgtcggcgaa cgtcacctcg ccgtcgcgcg aacggtggag
     2401 cacctccacg gtcgacgcga gctggtccat ccggtcggct ccggtccagt acgggaggcc
     2461 gaacgccagg ttctcggccg cgctcgcctg gtcaccgctg ccgatcgcca cgcagaggcg
     2521 gccgggcgct atctcggaga ccgtcgccgc catcttcgcc agcaccgcgg ggtgacgaag
     2581 cgacgagcgc gtgacgagcg tcccgaccgt cgcttccttc gtgcgcgcgg caacccacgc
     2641 cagcgccgtc cacccttcga ggatcggccg tcgcttcgcc ccgagcggcc agaggtggtc
     2701 gaagacccat agcgagtcga agccgaggcc ctccgcgcag cgcactccgt ctgtgaagac
     2761 cgacgcgtcg tcggtgaact gcgggagcgt gacgccgagc cggacgtccc tcacgacgcg
     2821 ctcaccatga gataccgcac caggaactcg gacacaccgg ccgtcaggag cgcgacgtag
     2881 tagaggccgg tggccgactg gatggacttg atcgcgacgc aacgccgggc cgcccacacg
     2941 actgctccgg tgaagccgac gagcgcgatc cagacgagga agaacgcgag cccgaaggac
     3001 tcgccgaagc cggggatgcc gaggaccgct ccgtccgtct cggcccccgc gagcttgccc
     3061 gcggcgacga gcccgagcac tcccgtcgcg atcgtcgcga cgagcgaaag agtggtcagg
     3121 cgcgcgaggg cccagggctt gaggcccggc tggttcaggt accagtggcc gaggagcatc
     3181 ccgttcgtca ccgcgccgag cagcgcggcc cccgcgacga cctcgaccgc cgccaggatc
     3241 acgggccagt cctcgagcaa cgacgcggcc gcccccaacg cgccgacgcc ggcaacggtc
     3301 gcggccacgc ccacgacgac cgcggggacg tcggtgttcg accactgcac gagcaggtag
     3361 aggcccacac cggccgcggt cgcgacgatc cacaccgccg ccccgccgct cacggggtcg
     3421 gcccccgagc gcatcgcgag gaccgcgagg atcgcgagcg cgacgccggt gtacgccgtg
     3481 atgcggaagt ggccacggcg cacgacgccc cacgactgcg tccacgcagc cgcgcccacg
     3541 agaccgacgg tccactcgag catgatcagc gctacgacgc ccgcggcgcc gctcaactcg
     3601 aagagaagcg tttcttgagc caccccggca tgtaggccgt ggagatgtcg aggatctggc
     3661 gaacgacctc gagcacgtcc tcgtcgccgt cccagtggtc gagcacgacc tcgtcgggga
     3721 cgcggttcag gatctggtcg agggcgaatg cgaggatcac gaggtcgtcg aggtggccga
     3781 gccccgggac gaactccggg atgaagtcga tcggagagag caggtagccc gcgaggacga
     3841 acgtagcggc cttgatacgc ggggggacgc gtgggtcgcg cgacaacctc cacacgagcc
     3901 gcccgagccg cggcacgagc aggacgtact ccttgaggct ggtgggcggg gggggcggct
     3961 tgaccggtgg ggtgctcatg ccgcgtcgac cgtctccgtc cacgcgagag cggccgcccc
     4021 cacgaggccc gcgtcaccgc gcaaagccgc gacgtcgagt cgcatcgcac gccgcctttg
     4081 tgccttcgtc atcagcgcga gctgatcccg cgcggccccc aggtacggct cgcccgcgtt
     4141 cacgacgctg ccgccgagga cgatgacctc cgggtcgaac acgttcgcta cgttcgacag
     4201 cccgatgccg agcgcggtgc cggccttgcg caggacatcg cgcgcggtct ggtcgtactc
     4261 gcgcgcggcc ttgctgacgg actcgcccgt gatctcgccg cccggcccgg cgaaggccgg
     4321 gatggtggag tccgggttgc tcccgagcgc gatgtgcgca agacgcgcga tcgcagtgcc
     4381 cgacgcgagc tgttcgagac agccgcgcag cccgcacggg cagggggggc cggaggggtc
     4441 gacgacgatg tggccgagct cggcccccgt gccggtcgcg ccgcgcacga gctctccgtt
     4501 ggcgacgatg ccgctcccga tcccggttcc cagcgtcagc atcacggcgt gctcggcgac
     4561 gcggcagcag ccgaacgtgc gctcgcccca cagcgccgcg ttcgcgtcgt tgtcgacgaa
     4621 tacggggagg ccgaaccgga gcgcgacggc cgtcgcgatc tgggggtcgt cgtagacgac
     4681 gttcggcgag aacgtgacgg cgccggcggc gaagtcgacg aacccggcgg cgccgacgcc
     4741 tatcgcccgg acctcgtttc cgcttccctc gagccgcggg agcaggtcct cgaggaccgc
     4801 gacg
//
LOCUS       DASQDO010000306         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1194714, whole genome shotgun sequence.
ACCESSION   DASQDO010000306 DASQDO010000000
VERSION     DASQDO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194714"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VEV43_08690"
     CDS             complement(<1..183)
                     /locus_tag="VEV43_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23637.1"
                     /translation="MTEADLVTREGMERRRFLKAAATVAWATPAILTLAANRAGAQSC
                     IPQGASCDACIGLNCCI"
     gene            complement(283..1095)
                     /locus_tag="VEV43_08695"
     CDS             complement(283..1095)
                     /locus_tag="VEV43_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23638.1"
                     /translation="MRIRVGLLDSVVEIRTDDDDAASLLSLLWGPMTTGDGPEPARTY
                     TLARGEDGGWIATAGDSVEAVHETLWGVTDALRYRMLEVCEERLERYVTLHAAAVARA
                     GDLVLLAGESGAGKTTLTLALLDAGWAYLSDDLAPVSVATGLVHPFPKPLGVKDPATW
                     GRVGDAFDGLSLGPPAGSFLVPATRWDVAPGPLPVRALLFPRFAAGAALEVERLSAAR
                     GAALASAHLRKLEPSTVSLLKRICAGAVCAGLTYGSSTDAIAAIEQLLNRYE"
     gene            complement(1092..>1590)
                     /locus_tag="VEV43_08700"
     CDS             complement(1092..>1590)
                     /locus_tag="VEV43_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23639.1"
                     /translation="GNAQLYADRDLGKVRSRLVRTIDAVLHAEETPAYLMTACRIDGR
                     PGLFARDVYNRAPFRIQVTRAGLELSDDTYVRMAPDGAFECEGWPPFRTEYIAVKNLP
                     GPRGRESENVNKGALLAFLFGILRVGDVGMVELHRLTSTIRTARVVAEDDPEQLVATL
                     RSLPR"
BASE COUNT          250 a          555 c          564 g          221 t
ORIGIN      
        1 gatgcaacag ttgaggccga tacaggcgtc gcacgaagcg ccctgcggga tgcacgactg
       61 ggcccccgca cggttcgcgg cgagcgtgag gatcgccgga gtagcccacg cgacggtggc
      121 cgcagccttg aggaacctgc gacgctccat gccttctcga gtgaccagat ccgcttccgt
      181 caacgtgagc tccttgggta cgtccgagtc cgccccggaa gcatagaggg agccccgcac
      241 ggcgcacaac cagaaaatag gaattacgta ggcgggttcg tcctattcgt accgattgag
      301 cagctgttcg atcgccgcga tcgcgtcggt gctcgatccg tacgtgaggc ctgcgcagac
      361 cgcgcccgcg cagatgcgct tcagcaacga gaccgtggac ggttcgagct tccggaggtg
      421 cgcgctcgca agcgctgcgc cgcgcgccgc cgacaggcgt tcgacctcga gggccgcccc
      481 cgctgcgaac ctcgggaaca gcagtgccct caccggcagc ggacccggcg cgacgtccca
      541 gcgcgtcgcg ggaacgagga acgagccggc cggggggccg agcgagagac cgtcgaacgc
      601 gtcgccgaca cgcccccacg tcgccgggtc cttcacgccg agtggcttgg ggaaggggtg
      661 cacgaggccg gtagcgaccg acaccggcgc gaggtcgtcg ctgagatagg cccatcccgc
      721 gtccagcaag gcgagcgtca gcgtcgtctt gccggcgccg gactcgccgg cgaggagcac
      781 gaggtcgccg gcgcgcgcca cggccgctgc gtggagggtg acgtagcgct ccaggcgctc
      841 ctcgcagacc tcgagcatcc ggtagcgcaa ggcgtcggtg acgccccaca gcgtctcgtg
      901 gacggcctcc acggagtcgc cggcggtcgc gatccagccg ccgtcctcac cgcgtgcgag
      961 cgtgtaggtg cgcgccggct cgggcccgtc gccggtcgtc atcggccccc acagcagcga
     1021 caggagcgag gccgcatcgt cgtcgtcggt gcggatctcg acgacgctgt ccagcagccc
     1081 gactctgatc ctcacctggg aagggagcgc agggtggcga cgagctgctc ggggtcgtcc
     1141 tcagcgacga cgcgggcggt gcggatcgtc gacgtgagcc ggtggagctc gaccatcccg
     1201 acgtcgccca ccctcaggat cccgaacagg aacgcgagca gcgccccctt gttcacgttc
     1261 tcggactcgc ggcctcgggg gccgggcagg ttcttcaccg cgatgtactc ggtgcggaag
     1321 gggggccagc cctcgcattc gaacgcgccg tccggtgcca tccgcacgta ggtgtcgtcg
     1381 gacagctcga gccccgcccg tgtcacctgg atacggaacg gcgcacggtt gtacacgtcg
     1441 cgcgcgaaca ggccgggccg tccgtcgata cggcacgcgg tcatcaggta cgcgggcgtc
     1501 tcctccgcgt gcaggaccgc gtcgatcgtg cgaacgagcc gcgagcggac cttgcccaag
     1561 tcgcggtccg cgtagagctg agcgttgccg
//
LOCUS       DASQDO010000307         5107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_827726, whole genome shotgun sequence.
ACCESSION   DASQDO010000307 DASQDO010000000
VERSION     DASQDO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827726"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VEV43_08705"
     CDS             complement(<1..773)
                     /locus_tag="VEV43_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23640.1"
                     /translation="MGAGGSTERRRTPGERVAVFLLRFVSALVAIAALAALTSDLVDK
                     ASAPEVPGWTGETPGEPEPEPEPEDDERRRRKAQLVHVNDPTVYDAQRKLDRCTRTRG
                     VMGASSRGPGLRDDIRKISGRVERLRDLDFRHRVETHLASQAEVGERFSRGFLNRYGE
                     REAERDQQILTALGLLPEGTDLRGLTAQLLNDGVAGFYNPRTGRLFAGSTGGGLTTFD
                     EVVLAHELDHALVDQALGLPGTLSRDPMLGDVMLAHQALA"
     gene            complement(823..2037)
                     /locus_tag="VEV43_08710"
     CDS             complement(823..2037)
                     /locus_tag="VEV43_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23641.1"
                     /translation="MRRSALIAVSIALVAAAACGDPAPPDRAAQEDLLYLEAAEGVAV
                     LAAGADIEPTPTAGLPSTDWTSVVTADANKLATSIEARDARTGKTQWTREIDGLKLDV
                     RVVSREAEMVALMPRNEPYLAGRTETTLVVTGRDVEPRTITLEGNYEPEAFSNDGGSV
                     FVLEYLPPKAPDSYRVRRLDLATEKVLGVYSVDAELQERMRGTARIQAMSPDGTYLYT
                     LYTTGGPGVGPRRAFVHVLNLDELWAHCIDLPPEFGNLRETEIAITVTPDSKRLYVND
                     VGSGAMAAVDTQSLEILDTASDVLTSGYEPLGVHDGRNTLYMARGPFLSGIDTRTFEE
                     GEQWTMPGRIKGVQVSSDLKKVYVALGREVITLDAATLDEIGRSDPPGVGRIQRMGRG
                     GRSLRQPILDKI"
     gene            complement(2102..4054)
                     /locus_tag="VEV43_08715"
     CDS             complement(2102..4054)
                     /locus_tag="VEV43_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HYP23642.1"
                     /translation="MRSKLISILAGLLLAASLTPGVAAGSQGPQKGKPERKAEKQPKA
                     VRIAAEHLEVDPDKVEVTDLYTSDHNDVTHVYLRQVVKGEEVLGAEATVNVQNGVVVF
                     AGEQFVDPKEATGDQVLDGPAALGAAIESLDEPALEVTRLPSLNYRALPDGTARLAWD
                     VEILTDDHWWNVSVDAETGEVVYRYDLVDSEQAEHIAARTARPEDGATASALLAQKPI
                     LPPEQTDDGSSYNVYAMPLESPNDGDRSIVENPADAVASPFGWHDTNGLPGPEFTITR
                     GNNANAYIDTIDGNGPDPASQPDGGRGLDFDHPIQTLDATPAAYRDAAVDNLFYWTNV
                     MHDVTFRYGFNEEAGNFQASNYSGAGRAGDEVQAEAQDGSGALNANFATPAEGVPGRM
                     QMYLWVDAFDSLSLQQDDVVRPYSHQVRDGDLDAGVIAHEYGHGISNRLVGGPRNVSC
                     LRVHDEREGEGWSDFWSYALTMRKGDDGRTPRGIGTYVVYHEDGRKSQGIRLAPYSTN
                     MKVNPATYDTIKTAAEPHGVGYVWASMLWDLYWNLVDEYGFNPNPYESWKTGGNNLAI
                     QLVVDGMKFAPCEPGFEDARDAIIAADQALTGNAAKGVPGRNECLIWRTFARRGLGVN
                     AKQGDVQLKSDGKQGFKVPAHCSGKG"
     gene            complement(4206..4568)
                     /gene="ssrA"
                     /locus_tag="VEV43_08720"
     tmRNA           complement(4206..4568)
                     /gene="ssrA"
                     /locus_tag="VEV43_08720"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4686..>5107)
                     /locus_tag="VEV43_08725"
     CDS             complement(4686..>5107)
                     /locus_tag="VEV43_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23643.1"
                     /translation="LQKKVADTLRWVAILIGLAASGLAVWTVLAAADTTSVARLVGKV
                     VAAIALYGVAGYVASQSAAHRRREEHAKRRELDLVAFGPFIADLEEPDRQLARKELVA
                     KLFGNDALDDEKKGGVLTQENVGLLGQLWEIFSKSRK"
BASE COUNT          699 a         1727 c         1803 g          878 t
ORIGIN      
        1 gcaagggcct ggtgcgcgag catcacgtcg ccgagcatcg ggtcgcgcga cagcgtcccc
       61 gggagcccga gggcctggtc gacgagcgcg tggtcgagct cgtgagccag gacgacctcg
      121 tcgaacgtcg tcagcccgcc accggtgctc cccgcgaaca gccggcccgt gcgcgggttg
      181 tagaagcccg cgactccgtc gttcaacagc tgcgcggtca acccacggag gtcggtgccc
      241 tcggggagga gccccagcgc ggtcaggatc tgctggtcgc gctccgcctc gcgctcgccg
      301 taccggttca ggaacccgcg cgagaaccgc tcccccacct cggcctggga cgcgaggtgg
      361 gtctcgaccc ggtgccggaa gtcgaggtcc ctcagccgct cgacgcggcc ggagatcttc
      421 cggatgtcgt cgcgcagtcc cggcccccgc gacgacgccc ccatcacccc gcgggtccgc
      481 gtgcagcggt cgagcttccg ttgcgcgtcg tagaccgtcg ggtcgttcac gtggacgagc
      541 tgggccttcc ggcgacgccg ctcgtcgtcc tcgggctcgg gctcgggctc gggctcgccg
      601 ggcgtctccc ccgtccaccc cgggacctcg ggcgccgacg ctttgtcgac gaggtcggac
      661 gtcagcgcag ccagggccgc gatcgccacg agggccgaca cgaacctgag gaggaagacc
      721 gcgacgcgct cgcccggcgt ccgccgccgc tcggtcgatc cgcccgcgcc cacgccggaa
      781 aggttcccac ggcgggggcc cgggcggggt cagcaggcgc agctagatct tgtcgagaat
      841 cggctgacgg aggctgcggc ccccgcggcc catccgctgg atgcggccga cgccgggcgg
      901 atccgaccgt ccgatctcgt cgagcgtggc cgcgtcgagc gtgatcacct cgcgcccgag
      961 ggcgacgtag accttcttga ggtccgaaga gacctgcacg cccttgatac ggccgggcat
     1021 cgtccactgc tcgccctctt cgaacgtccg cgtgtcgatc cccgagagga acgggccgcg
     1081 cgccatgtag agggtgttgc gaccgtcgtg gacgcccagc ggctcgtacc ccgacgtgag
     1141 gacgtcgctc gcggtatcga ggatctcgag cgactgcgtg tcgaccgcgg ccatcgcgcc
     1201 ggagccgacg tcgttgacgt acaggcgctt cgagtccggc gtgaccgtga tcgctatctc
     1261 ggtctcccgc aggttgccga actccggcgg gaggtcgatg cagtgcgccc acagctcgtc
     1321 gaggttgagg acgtggacga acgcgcggcg ggggccgacc cccgggccgc ccgtcgtgta
     1381 gagcgtgtag aggtacgtcc cgtcggggct catcgcctgg atgcgcgcgg tgccgcgcat
     1441 gcgctcttgc agctcggcgt cgacgctgta gacgccgagg accttctcgg tcgcaaggtc
     1501 gagccggcgc acgcggtagc tgtcgggtgc cttggggggc aggtactcga gcacgaagac
     1561 gctgccgccg tcgttcgaga acgcctcggg ctcgtagttg ccttcgagcg tgatcgtgcg
     1621 cggctcgacg tcgcggccgg tgacgacgag cgtcgtctcg gtgcggcccg cgaggtaggg
     1681 ctcgttgcga ggcatgagcg cgaccatctc ggcctcgcgc gagacgaccc tgacgtcgag
     1741 cttcaggccg tcgatctcgc gcgtccactg cgtcttgccg gtgcgggcgt cgcgcgcctc
     1801 gatgctcgtc gcgagcttgt tcgcgtccgc ggtcacgacg ctcgtccagt ccgtggacgg
     1861 gagcccggcg gtcggcgtgg gctcgatgtc cgcgcccgcg gcgagcacgg cgacgccctc
     1921 ggcggcctcc aggtacaaga ggtcctcctg ggcggcccgg tccggggggg ccgggtcccc
     1981 gcaggcggcg gctgcgacta gggctatcga aacggcgatg agcgcggatc ggcgcatggg
     2041 tcccctcctc gtcgggttcg tccgaagaag gagactgagc tgcggggcgg ttggttctcc
     2101 cttatccctt cccgctgcag tgggccggga ccttgaagcc ctgcttgccg tccgacttga
     2161 gctgcacgtc gccctgcttc gcgttcacgc cgagcccccg gcgggcgaac gtgcgccaga
     2221 tcaggcactc gttgcggccc gggacgccct tggcggcgtt cccggtgagg gcctggtcgg
     2281 ccgcgatgat cgcgtcgcgg gcgtcctcga agcccggctc gcacggtgcg aacttcatcc
     2341 cgtcgacgac gagctggatc gcgaggttgt tgccgcccgt cttccacgac tcgtacgggt
     2401 tcgggttgaa gccgtactcg tcgacgaggt tccagtagag gtcccacagc atcgacgccc
     2461 agacgtagcc gacgccgtgg ggctcggccg cggtcttgat cgtgtcgtag gtcgccgggt
     2521 tgaccttcat gttcgtcgag tacggagcga ggcggatgcc ctgcgacttg cggccgtcct
     2581 cgtggtacac gacgtacgta ccgatgccgc gcggcgtgcg cccgtcgtcg cccttgcgca
     2641 tcgtgagcgc gtacgaccag aagtcgctcc agccctcgcc ctctcgctcg tcgtgcactc
     2701 gaaggcacga gacgttgcgg gggccgccga cgagacggtt cgagatgccg tggccgtact
     2761 cgtgcgcgat gacgccggcg tcgaggtcgc cgtcgcggac ctggtggctg tacggacgga
     2821 cgacgtcgtc ctgctgcagg ctcaagctgt cgaacgcgtc gacccagagg tacatctgca
     2881 tgcggccggg gacgccttcc gccggcgttg cgaagttcgc gttgagggcc ccgctcccgt
     2941 cctgcgcctc ggcctggacc tcgtcgccgg cgcgcccggc gcccgagtag ttcgaggcct
     3001 ggaagttgcc ggcctcttcg ttgaagccgt agcggaacgt cacgtcgtgc atcacgttcg
     3061 tccagtagaa gaggttgtcg accgccgcgt cgcggtaggc cgcgggcgtc gcgtcgaggg
     3121 tctggatcgg gtggtcgaag tcgaggccgc ggccgccgtc cggctggctc gcggggtcgg
     3181 gcccgttgcc gtcgatcgtg tctatgtagg cgttggcgtt gttgccgcgg gtgatcgtga
     3241 actcgggccc ggggaggccg ttcgtgtcgt gccagccgaa cggcgacgcg accgcgtcgg
     3301 cggggttctc gacgatcgag cggtcgccgt cgttcgggct ctcgagcggc atcgcgtaga
     3361 cgttgtagga ggagccgtcg tcggtctgct ccggcgggag gatcggcttc tgggcgagga
     3421 gggccgaggc cgtggcgccg tcctcgggcc gcgccgtgcg ggccgcgatg tgctccgcct
     3481 gctcgctgtc gacgaggtcg tagcggtaga cgacctcgcc ggtctccgcg tcgacggaga
     3541 cgttccacca gtggtcgtcg gtgaggatct cgacgtccca ggcgagtcgg gcggtgccgt
     3601 ccgggagggc ccggtagttg agcgagggca gcctcgtgac ctcgagggcg ggctcgtcga
     3661 gcgactcgat ggccgcaccg agcgccgccg gcccgtcgag gacctggtcg ccggtggctt
     3721 ccttcgggtc gacgaactgc tcgccggcga agacgacgac gccgttctgg acgttgaccg
     3781 tggcctcggc ccccaggacc tcctcgccct tgacgacctg gcggaggtag acgtgggtga
     3841 cgtcgttgtg gtccgaggtg tagagatcgg tgacctcgac cttgtcgggg tcgacctcga
     3901 ggtgctcggc cgcgatgcgg acggccttgg gctgcttctc ggccttcctc tcgggcttgc
     3961 ccttctgggg cccttgggag ccggcggcga cgccgggggt gagggaggcc gctaggagca
     4021 gcccggccag gatcgagatc aacttggaac gcatcacgcc tccgcagctt gaagagcttt
     4081 gcccgctttg ccgcgctttg cggcctgggg gaggattcgg cagcgggggc tcatgccctg
     4141 cccggttctc acccgaagga gtgatcaggg cgcgggcaaa tccgcctggt ctccgaggcg
     4201 ggtcgtggtg gagctgccgg gaatcgaacc cgggtcctcc gaccccgtgg caggacttct
     4261 acgagcgtag tctgcggtca gttttcggcc ccggccggcc cgcagacggc ggcggccgtg
     4321 gcctagctca gactagattt cccgtccttc gctccgagcg gactcgggac ggtgagcctg
     4381 ctaggcgacg ccggatcccg ggggcgcagg cgatccccgg gccgacggcc attgctaagg
     4441 aatggcaacc gtgcggttag gcagcggcat ttattgttgt tccgagaata tttaacgagg
     4501 tcacacccgg agtcctcggc tcgcttcccc tgcctcggtc tgatcagagt cgataccagt
     4561 tcagccccga gctgggttct tttcaatgtt ccattagcca gtgtagcgat cccttgacgt
     4621 tcgacactca gaacatcaaa ccgacgctca cgtccaagcg cgcaggtggg agctgctaga
     4681 tcccgctact ttcgactttt cgagaaaatt tcccacaatt gacccaagag gccaacgttc
     4741 tcctgcgtaa gaacgccccc ctttttctca tcgtccagag cgtcgttgcc aaagagcttc
     4801 gcaaccagtt ccttccgcgc caattgacgg tcaggttcct ccaggtcagc aatgaagggt
     4861 ccaaacgcca caaggtcgag ttctcggcgc ttagcgtgct cctcgcgccg acgatgcgcc
     4921 gccgactgac tcgcaacgta cccggcgacc ccatagagag cgatcgccgc aacgaccttt
     4981 cccacaagtc gggccaccga cgtcgtatcc gctgccgcga gaacagtcca cactgcgaga
     5041 cccgacgcag cgagaccaat gagaatcgcc acccaccgga gcgtgtccgc gaccttcttc
     5101 tggagct
//
LOCUS       DASQDO010000308         1739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1195020, whole genome shotgun sequence.
ACCESSION   DASQDO010000308 DASQDO010000000
VERSION     DASQDO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195020"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VEV43_08730"
     CDS             complement(<1..685)
                     /locus_tag="VEV43_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP23644.1"
                     /translation="MTTDAKRLEGKVALVTGAGSGIGEAVAHRLARDGATVIVADLDD
                     VGAKRVAEDIGYAAHAEVADVSEPDSMRELIDRVVAEHGRLDVAVNNAGIGGVSAPVG
                     EYPLESWRQVLSVNLDGVFYSMRYEIPAMQRNGGGSIVNMGSVLSSVGFGQSGAYVAA
                     KHGVTGLTKAAAIEYSKDGIRVNAVGPGFIETPLLRKPEAAEIVKNVTALHPIGRLGR
                     PEEVANLVAF"
     gene            775..1257
                     /locus_tag="VEV43_08735"
     CDS             775..1257
                     /locus_tag="VEV43_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYP23645.1"
                     /translation="MSRVGKQAEPRRFRELVRGAGRDRAASRVVVSVLRADSKASQTL
                     ERALGAVGLSLPQFNVLMVLGASPDGALPIYELNARLVTSPPNMTWISDRMEERGFVH
                     KRRDETDRRVVVIELTEGGWAALEGAAPVVFEAEKELVAGYSRAELKDLGNLLGPFLD
                     "
     gene            complement(1277..>1739)
                     /locus_tag="VEV43_08740"
     CDS             complement(1277..>1739)
                     /locus_tag="VEV43_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23646.1"
                     /translation="GGLTELEDGVKLYEGLTAPPVFWPILLTIQGRANLLAGRVEAAA
                     ALFAEADVAATAGDPVVIDLAIARGDVLLAGASPDTAGAEESYERAAGMARTFGLRMA
                     ELEAATRLAQLRAGTAGDEDSLRRLRGLLDAFTEGHSTPQLTAAAALARGH"
BASE COUNT          255 a          608 c          594 g          282 t
ORIGIN      
        1 ggaaggcgac caggttcgcg acctcttcgg gccggcccag ccgcccgatg ggatgcaggg
       61 cagtcacgtt cttgacgatc tcggccgcct ccggcttcct cagcaacggg gtctcgatga
      121 acccggggcc gaccgcgttc acgcggatgc cgtccttcga gtattcgatc gccgcggctt
      181 tcgtgagacc cgtcacgccg tgtttggcgg cgacgtacgc gccggactga ccgaacccga
      241 ccgagctcag caccgagccc atgttcacga tcgacccgcc gccgttgcgc tgcatcgcgg
      301 ggatctcgta gcgcatcgag tagaagaccc cgtcgaggtt caccgacaag acctgcctcc
      361 agctctcgag cgggtactcg ccgaccgggg cggacacgcc accgatgccg gcgttgttga
      421 ctgcgacgtc gaggcggccg tgctctgcga cgacgcgatc gatcagctcg cgcatcgagt
      481 ccggctcgct gacgtctgcg acctccgcgt gggccgcgta gccgatgtct tcggcgaccc
      541 tctttgcccc gacgtcgtcg aggtcggcga cgatcacggt tgctccgtcc cgggccaggc
      601 ggtgggcgac tgcctcaccg atccccgagc cggcgcccgt cacgagcgcc accttgcctt
      661 cgagccgctt ggcatccgtc gtcatcgcgc tctccctcca tcgttcgttg ccgtcgtcat
      721 gcacaacaag gcccgctcgg atatatttcc aactcgaaat atctactaag aggggtgtcc
      781 agggtgggca agcaggccga gccgcgcagg ttccgggagc tcgtgcgggg ggcggggcgg
      841 gaccgggccg cgagccgcgt cgtggtgtcg gttctgcgcg ccgactcgaa agcgtcgcag
      901 acgttggagc gcgccctcgg cgcagtcgga ttgtcgttgc cgcagttcaa cgtgctgatg
      961 gtcctggggg cgtcgccgga cggggcgctg ccgatctacg agctgaacgc gcggctcgtg
     1021 acctcgcccc cgaacatgac gtggatctcg gaccggatgg aggagcgcgg gttcgtccat
     1081 aagcggcgcg acgagacgga ccgcagggtc gtggtgatcg agctcacgga agggggctgg
     1141 gccgcgctcg agggggccgc gcccgtggtg ttcgaggccg agaaggagct ggtcgcgggt
     1201 tactcacgcg ccgagctgaa ggacttgggc aacctgttgg gcccgttcct cgactgatag
     1261 cgaagggagc ggagcgtcag tgcccgcgcg ccaacgccgc ggctgcggtg agctgcggcg
     1321 tcgaatggcc ttccgtaaag gcgtcgagca ggccccggag tctccgcagc gagtcttcgt
     1381 ctcccgccgt gcccgcgcgg agctgcgcca gcctcgtcgc cgcctcgagc tcggccatcc
     1441 gcagcccgaa agtcctcgcc atgccggcag cccgctcgta cgattcctcg gctcccgccg
     1501 tgtcgggaga cgcaccagcg agcagcacgt cgccgcgcgc gatcgcgaga tcgatgacca
     1561 ccgggtcgcc cgcggtagcg gccacgtccg cttcggcgaa cagcgcggcg gctgcctcga
     1621 cccggcccgc caggaggttc gcccgtccct ggatggtcaa caatatcggc cagaagaccg
     1681 gcggagccgt caatccctcg tagagcttca caccgtcctc gagctccgtc agtcccccc
//
LOCUS       DASQDO010000309         9472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_889291, whole genome shotgun sequence.
ACCESSION   DASQDO010000309 DASQDO010000000
VERSION     DASQDO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889291"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VEV43_08745"
     CDS             complement(<1..531)
                     /locus_tag="VEV43_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23647.1"
                     /translation="MSHYAEPGQPYRTPGKAGLWAGGFWLSLGGISVALAIDDRALAD
                     DVEFASRQWRTAFGVVGAANALFGIRIALRALGGDRRQVTPESLRRSRILGGVLILVG
                     LWYQIPAWSDGAVRIGVGLDGWAGGVYGVGGLSLMLVGLALQVDPTRFLRHERVREGD
                     GRTVTATILRATDLGSL"
     gene            complement(539..1288)
                     /locus_tag="VEV43_08750"
     CDS             complement(539..1288)
                     /locus_tag="VEV43_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23648.1"
                     /translation="MNGPVQPGMVVDPVVRPSGLGGFVFALLDLAIGGVAAGLALNEG
                     FAAGKAAFATDAWRTTLAVTGIALSILAVVGLVRVAGGQPRTLTPGLRRRARLTGVVL
                     MLVGVWLLACATVDAVARETVSFDSWTSPLFAAGGLYLVVIGLSLQLNVTRVLRKQQL
                     EGGAGVPGTATIVGVSETGSSTETGPVMRLDLRIDVNGRVYEATEEKQADPSSLALFT
                     PGATLDVLVDYVDPRVFRLDWKTWTAPSAGG"
     gene            1394..2215
                     /locus_tag="VEV43_08755"
     CDS             1394..2215
                     /locus_tag="VEV43_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYP23649.1"
                     /translation="MTETKQDYIYDPSSESELTRLRKLEALNDPATIAHLEAVGVAPG
                     WRCLEVGGGAGSIARWLCERVGAEGRVVATDLDVRFLEEIDAPNLEVRRHDILEDELE
                     QGAYDLVHSRFLLEHLAGYREALAKMVDALAPGGWIVVEDVDFALAIMGDADERPGYP
                     PESIPVAAEMSQRLGGLAKVRGIQAELGRRLPALLAEAGLEEIGAEGRTNLMWTGTEE
                     AELGRLSLERVTRLAADAGVITEEDRDRYMKVVTEPGQASFSPLRFGAWGRKPRG"
     gene            complement(2212..3864)
                     /locus_tag="VEV43_08760"
     CDS             complement(2212..3864)
                     /locus_tag="VEV43_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23650.1"
                     /translation="MKRVTAWALLMLLAVPVAPAHAAPTCRGRRATIHDPRARWLRGT
                     NGDDVIVGSSANWFVDALKGDDLICQAGAHDADDIDGGRGNDRIIGTSRLTDLIGGPG
                     NDVLTSKEPLSMDGGPGDDELRGGTGLSGGPGDDTLIARRGKEWDTYFGPGAGRDFVD
                     STNGAGFVAYGGAPRGVRVRLDRGTARGWGHDTLVDVEHASGTRHDDVLVGTGDVDSG
                     GFDRNDLSGLSGDDVLIGLGGPDHLDGVAGADVARGGPGEDWLRPIYGEPPGAEGDRL
                     YGGEGYDRLEGSSGPDLLFGGPDDDVLAGRGHHNETYGGPGNDHLKFGGHIEKLSGGP
                     GSDTLDFTVFGPVHADLRDQTARLGNFVLELEGIESLWGSYYGDTLIGDDGPNVLFDG
                     GNYSADGQEDVLDALGGDDRVALSGDRSDQAAGDTFDARGGEGRDTLQLQWASTIDLA
                     AGTARVASSIGVVGGFEDVAGSREDDSLAGDDGPNHFIGYRGDDALFGRGGEDDLDGD
                     DGTDSADGGDGTDRCAAETTTACEQSLPLNARLLVSFLRVSS"
     gene            4034..5524
                     /locus_tag="VEV43_08765"
     CDS             4034..5524
                     /locus_tag="VEV43_08765"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HYP23651.1"
                     /translation="MYSNGMFIAGEFCEAASGRTDDVLNPATEEVCGSVPRAEEADVD
                     RAVQAAREAFEDGRWSELGPGERAAVLWKMGELVEERADELIPLEIAPSGKTMKLVAD
                     GDVPMAADQLKYFGGIARALAGAAAGEYLPGYTSWLRREPVGVIAQVTPWNYPLMMAV
                     WKIGPALAAGNTIVLKPAPWTPFTTLELARIAKDAGLPDGVLNVVTGRGQECGQPLVS
                     HPEVDMVSLTGSTDTATSIMEAAAPTIKRLHFELGGKAPFIVYDDADLEAAAQGCIVG
                     SFINTGQDCTAVTRIYVQRAVWDEFVARVSDLTAELRLGDPMERTADMGPLINEAQRE
                     RVEGFVERARSQGAKVLMGGGRPEGFDKGYWFEPTLISGADQQAEIVQHEVFGPAPTL
                     LPFETEDEVLAKANDVKYGLAASVWTRDSFKSMRAAKALRFGTVWINDHIPLTPEMPH
                     GGFKQSGFGKDMSLESLEEYTVTKHVMAELSGVPRKDWYYVVAGDE"
     gene            5527..6948
                     /locus_tag="VEV43_08770"
     CDS             5527..6948
                     /locus_tag="VEV43_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYP23652.1"
                     /translation="MKKFLVAGEWRGGDEAFEVNSPYDGEVVASVGSPSEKDVEDAVA
                     AAADAFDETRKLPLHARAEALAHISKRLAERADEVAELIAREGGKPLKWAKVEAARAV
                     STFRWAAEECRRDGGELMRFDSDAALGSRAGIVRRFPLGPVLAITPFNFPVNLVAHKM
                     APALAVGAPIVIKPATKTPLGALLLGELFCETDLPPGMLSVLPIGGSEAGELAKDTRF
                     AKLSFTGSSEVGWNLKTADPRKVVTLELGGNAGVIVHSDADLDKAAQRIAFGGYYQAG
                     QSCISVQRVLVQESVADDLSERLVAEVGKLKTGDPLDPETEVGPLIDHDALERVSAWV
                     DEAVQAGAEVLVGGKREDPFYVPTVLSRTRPEMKVRCEEIFGPVTTIGTYGDFEEAIA
                     EVNESKYGLQAGVFTSDVQRLFLAFRDIEVGGVIANDVSAFRADQMPYGGTKDSGSGR
                     EGLRWAMQEMTETRVLVLSDVPL"
     gene            7017..7289
                     /locus_tag="VEV43_08775"
     CDS             7017..7289
                     /locus_tag="VEV43_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23653.1"
                     /translation="MTQGVLVCEGSSWPRRLGWRYRAITFGHVVLSVGDLDDRTLAHE
                     LVHVRQYERWGILLWPAYGLASVWAKLRGGDAYWDNHFERQARGEA"
     gene            7286..8221
                     /locus_tag="VEV43_08780"
     CDS             7286..8221
                     /locus_tag="VEV43_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23654.1"
                     /translation="MRRLRLAAAAVVFATVAVPGTAAAPLPAAPDCRIFPESNVWNRR
                     VDDLPVHARSRAIIRSIGRSEGLHPDFGSGRWDGGPIGIPFTVVGDGQRKSRVRFQYA
                     DESDKGPYPIPKDVPIEGGRQSDGDRHALILQKDECRLYELYALRRRDGRWRAGSGAI
                     WDLDSNELRPKGWTSADAAGLPILPGLARWDEVKRGVIDHALRFTVSRTRAKYIYPAR
                     HYASDSDDANLPPMGLRLRLKKSFPVKGFPRQARIVLVALKRYGMIVADNGSDWFISG
                     APNPHWDNDALRTLGEVKGSDFVVVDTSELRPWKD"
     gene            complement(8266..>9472)
                     /locus_tag="VEV43_08785"
     CDS             complement(8266..>9472)
                     /locus_tag="VEV43_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23655.1"
                     /translation="STPAAASWPGSNGDIFMVENEGNETSIVRYNQAGEVVGSVAENL
                     TQFSNFSTDAAGERLVYSSNGELVLVDLESMEPTNLTESPAIETDPTFSFDGRYVVYV
                     SNGDGDQDIYMWDLTDPSMGPYNLTNNDVDDWAPAWGNDGTGHGDLIAYVSLQNSAAQ
                     GESDIWTMTPGGTDKTDLTNNDVPDFGPNWHPSFDMLAYSSQDEAGRDQIHTMSWDGS
                     GKDQLTSDDNYNYEPAWSPDGTKIAFTKQSPAVNSVGRIYIRSGSPGGTETPLTPAGP
                     GTDRADWQAAPGGCEPNCGGEPIRPTILFSLKRHVVVSGSVIGQGSDTEAGGTCGVGT
                     PVNIQRKIDGKFKTIASLATDVDGKFSRKVPDRTGQYRVVSTRYTDAETGTECLPSQA
                     AAKTHRHRR"
BASE COUNT         1396 a         3275 c         3350 g         1451 t
ORIGIN      
        1 caggctgccg aggtcggtgg cgcgcaggat cgtcgcggtg acggtcctgc cgtcgccctc
       61 ccgaacgcgc tcgtgcctca gaaatcgtgt gggatcgacc tggagcgcca gccctaccag
      121 catcagagag agccccccta ccccgtagac gccgccggcc cacccgtcga gccccacgcc
      181 gatccgcacg gctccgtcgg accaggcggg gatctggtac cagaggccga ccaggatcag
      241 gacgcccccg aggatgcggc tccggcgcaa cgactcgggt gtgacctgcc gccggtcgcc
      301 gcctagagcg cgcagcgcga tcctgatccc gaacaaggcg ttcgctgcac cgaccactcc
      361 gaacgcagtc ctccactgcc ggctcgcgaa ctccacgtcg tcggcgaggg ccctgtcgtc
      421 gatcgcgagc gcgaccgaga tacctcccaa cgaaagccag aagcccccgg cccagaggcc
      481 tgccttgccc ggtgtccggt acggctggcc cggttcggcg tagtggctca tcgcggcgtc
      541 agcctccggc actcggggcc gtccaggtct tccagtcgag ccggaagacc cgcggatcca
      601 cgtagtcgac gagcacgtcg agggttgcgc cgggcgtgaa cagcgcgagc gacgacgggt
      661 ccgcctgctt ctcctcggtc gcctcgtaga cgcgtccgtt gacgtcgatc cggagatcga
      721 gtcgcatcac gggccccgtc tcggtgctgg agccggtctc ggagacgccc acgatcgtcg
      781 ccgtccccgg gacgcccgcc ccgccctcga gctgctgctt cctcaagacc ctcgtgacgt
      841 tcagctggag cgacagcccg atgacgacga ggtacagccc tccggccgcg aacaacgggc
      901 tcgtccacga gtcgaacgac acggtctctc gggcgacggc gtcgacggtc gcgcacgcga
      961 gcagccacac tccgacgagc atcagcacga cgcccgtcag gcgtgcgcgc cgccgcagcc
     1021 cgggcgtcag ggttcgcggc tgtccgccgg cgacgcggac gaggcccacg acggcgagga
     1081 tcgacagagc gatccccgtt accgcgagcg tcgtcctcca tgcgtcggtg gcgaaggcgg
     1141 ccttccctgc cgcgaagccc tcgttcagcg cgagcccggc tgcgaccccg ccgatcgcta
     1201 gatcgagcag cgcgaagacg aacccgccga ggccggacgg gcgaacgacc gggtcgacga
     1261 ccatgcccgg ctggaccgga ccgttcacga acccgtcacg acgcgaagcc tcatccgccc
     1321 gccctttgct cgtacgccgt cgggagaaga ctgccagaaa tcgcagccgc tcgccttggt
     1381 gcactatgcg ggcatgaccg agacgaagca ggactacatc tacgacccga gctccgagag
     1441 cgagctgacg cgcctccgca agctcgaagc cctcaacgac cccgcgacga tcgcccacct
     1501 cgaggcggtg ggggtggcgc cgggctggcg ctgcctcgag gtcgggggtg gagccgggtc
     1561 gatcgcacgc tggctctgcg agcgcgtagg ggccgagggc cgcgttgtcg cgaccgacct
     1621 cgacgtgagg ttcctcgaag agatcgacgc gccgaacctc gaggtgcgcc gccacgacat
     1681 cctggaggac gagctggagc aaggtgcata cgacctcgtc cactcgcgct tcttgctcga
     1741 gcatctggcg gggtaccgcg aggcgttggc gaagatggtc gacgcgctcg cgcccggcgg
     1801 atggatcgtc gtcgaggacg tcgacttcgc gctcgcgatc atgggcgacg ccgacgaacg
     1861 tccgggctac ccgcccgaga gcatcccggt cgcggccgag atgtcgcaac gcctcggcgg
     1921 cttggcgaag gtgcgcggca tccaggccga gctcggtcgc cggctcccgg cgctgctcgc
     1981 ggaggcgggg ctcgaggaga tcggagcaga gggccgcact aacctgatgt ggacggggac
     2041 cgaggaggcc gagctggggc gcctgtcgct ggaacgcgtg accaggctcg cggcggacgc
     2101 aggcgtcatc accgaggaag accgcgaccg gtacatgaag gtcgtcacgg agccgggtca
     2161 ggcgtcgttc tcgccgctgc gtttcggggc gtgggggcgc aagccgcgcg gctagctcga
     2221 gacccgtaag aacgacacga gcaaacgcgc gtttagcggc aacgactgct cgcacgcggt
     2281 cgtcgtctcg gccgcgcatc ggtcggtccc gtcaccgccg tccgccgaat ccgtgccgtc
     2341 gtccccgtcg aggtcgtcct caccgccgcg gccgaacagc gcgtcgtcgc cccggtaccc
     2401 gatgaagtgg ttgggcccgt cgtccccggc cagcgagtcg tcctcgcggc tcccggccac
     2461 gtcctcgaag cccccgacga cgccgatcga agaagccacc cgcgcggtcc ccgcggcgag
     2521 gtcgatcgtg gacgcccatt gcagttgcag ggtgtcgcga ccttcgcccc cgcgcgcgtc
     2581 gaaggtgtcg ccggccgctt gatccgaccg atcgccgctc agcgcgacgc gatcgtcgcc
     2641 gccgagcgcg tcgaggacgt cttcctgacc gtccgcgctg tagttgccgc cgtcgaagag
     2701 gacgttcggc ccgtcgtcgc cgatcagcgt gtcgccgtag tagctgcccc acaggctctc
     2761 gatcccctcc agctcgagga cgaagttgcc gaggcgcgcg gtctggtcgc gcaggtcggc
     2821 gtggacgggg ccgaagaccg tgaagtcgag ggtgtcgctg cccggccccc cgctcagctt
     2881 ctcgatgtga ccgccgaact tcaggtggtc gttacccggg cccccgtacg tctcgttgtg
     2941 gtgcccccgg cccgcgagca cgtcgtcgtc cggaccgccg aagaggaggt cgggaccgct
     3001 gcttccctcg aggcggtcgt agccttctcc tccgtagagg cgatcgccct ccgcgccggg
     3061 cggttccccg tagatcgggc ggagccagtc ctccccggga ccgccacggg cgacgtcggc
     3121 gcccgcgacg ccgtccaggt ggtccggccc cccgagcccg atgaggacgt cgtcgccgct
     3181 cagcccgctc aggtcgtttc tgtcgaaccc cccggagtcg acgtctcccg tccctacgag
     3241 cacgtcgtcg tgcctcgttc cgcttgcgtg ctcgacgtcg accagggtgt cgtgccccca
     3301 gccgcgcgcg gtccctcggt cgagacgcac gcgcacgcct cgcggcgcgc ctccgtacgc
     3361 gacgaacccg gccccgttgg tgctgtcgac gaagtcgcgg ccggcgccgg gaccgaagta
     3421 ggtgtcccat tccttcccgc gccgcgctat gagcgtgtcg tcgcccggcc cccccgacag
     3481 gcccgttcct ccgcggagct cgtcgtcgcc ggggccgccg tccatcgaca gcggctcctt
     3541 cgaggtcaag acgtcgttcc ccgggccgcc gatcaggtcc gtgagccggc tggtgccgat
     3601 gatccggtcg tttccgcgac cgccgtctat gtcgtccgcg tcgtgcgccc cggcctggca
     3661 gatcagatcg tcgcctttca gggcgtcgac gaaccagttc gcagacgagc cgacgatcac
     3721 gtcgtcgccg ttcgtgccgc gcagccatcg ggcgcgggga tcgtggatcg tggcgcggcg
     3781 cccccggcac gtcggggcgg cgtgggcggg ggcgaccggg acggcgagga gcatgagcag
     3841 agcccaagcc gtgactcgct tcaactgctc cagcctccta ggtcggttcg ccaccgcgat
     3901 gactcaccgt ggcggtgagc cgttccatgc gccagtctgt acaaccagcc tcccgggctt
     3961 gggcccccga ggctcgcgcc tccccggccc ccgtcgctag catctctctg aggagaccca
     4021 cgaggaggcg tccatgtatt ccaacggcat gttcatcgcg ggggagttct gcgaggcggc
     4081 gtcggggcgc accgatgacg tgctgaaccc cgccaccgaa gaagtatgcg ggagcgtccc
     4141 gcgcgccgaa gaggccgacg tcgaccgcgc cgtgcaggcg gcgcgcgagg cgttcgagga
     4201 cgggcggtgg tccgagctcg gccccggtga gcgcgccgcg gtcctctgga agatgggcga
     4261 gctcgtcgag gagcgcgccg acgagctgat ccctctcgag atcgcgccgt cgggcaagac
     4321 gatgaagctc gtagcggacg gcgacgtgcc gatggccgcg gaccagctga agtacttcgg
     4381 cgggatcgca cgcgcgctcg cgggggccgc ggccggcgag tacctgcccg gctacacgtc
     4441 atggctgcgg cgcgagccgg tcggcgtcat cgcgcaggtc acgccgtgga actacccgct
     4501 gatgatggcg gtgtggaaga tcggcccggc cctcgcggcc ggcaacacga tcgtcctcaa
     4561 gcccgcgccg tggaccccgt tcaccacgct tgagctcgca cgcatcgcga aggacgcggg
     4621 gctccccgac ggagtgctca acgtcgtcac cggccgcggg caggagtgcg gacagccgct
     4681 ggtcagccac ccggaggtcg acatggtgtc gctcaccggc tcgaccgaca cggcgacgtc
     4741 gatcatggaa gcggccgcgc cgacgatcaa gcggctgcac ttcgagctcg ggggcaaggc
     4801 accgttcatc gtctacgacg acgccgacct cgaggccgcc gcgcagggtt gcatcgtcgg
     4861 ctcgttcatc aacaccggcc aggactgcac ggccgtgacg cgcatctacg tgcagcgcgc
     4921 ggtgtgggat gagttcgtcg cgcgggtctc ggacctgacc gcggagctcc ggctcggcga
     4981 cccgatggaa agaacggccg acatgggccc cctgatcaac gaggcgcagc gcgagcgcgt
     5041 cgagggcttc gtcgagcggg cgcgctcgca gggggcgaag gtgctgatgg gcggcggcag
     5101 gcccgagggc ttcgacaagg gctactggtt cgagccgacg ctgatctccg gcgccgacca
     5161 gcaggcggag atcgtgcagc acgaggtctt cggcccggcc cccacgttgc tcccgttcga
     5221 gaccgaggac gaggtgctgg ccaaggcgaa cgacgtgaag tacgggctgg ccgccagcgt
     5281 ctggacgcgt gactcgttca agtcgatgcg ggcggcgaag gctctgcgct tcggcaccgt
     5341 ctggatcaac gaccacatcc ccctgacccc cgagatgccg cacggtggct tcaagcagtc
     5401 cgggttcggc aaggacatga gcctggagtc gctcgaggag tacacggtga ccaagcacgt
     5461 gatggccgag ctctcgggcg tgccgcgcaa ggactggtac tacgtcgtgg cgggagacga
     5521 gtagccgtga agaagttcct cgtagcgggg gagtggcgcg gcggcgacga ggcattcgaa
     5581 gtaaatagcc cgtacgacgg cgaggtcgtc gcgtcggtcg ggtctccgtc ggagaaggac
     5641 gtcgaggacg cggtcgcagc ggcggcggac gccttcgacg agacgaggaa gctgccgctc
     5701 cacgcacggg cggaagccct cgcgcacatc tccaagcgtc tggccgagcg cgccgacgag
     5761 gtcgcggagc tgatcgcacg cgagggcggc aagccgctca agtgggcgaa ggtcgaagcg
     5821 gcacgtgcgg tctcgacgtt ccgctgggcc gcggaggagt gccggcgcga cggcggcgag
     5881 ctcatgcggt tcgactccga cgcagcgctc ggctcccgcg cgggcatcgt tcggaggttc
     5941 ccgctcggcc cagtgctagc gatcacgccg ttcaacttcc cggtgaacct cgtcgcgcac
     6001 aagatggctc cggcgctcgc cgtcggcgca ccgatcgtga tcaagccggc gacgaagacc
     6061 ccgctgggcg cgttgcttct cggcgagctg ttctgcgaga ccgacctccc gccggggatg
     6121 ctctcggtcc ttcccatagg aggaagcgag gccggtgagc tggcgaagga cacccgcttc
     6181 gcgaagctct ctttcaccgg ctcgtccgag gtcggctgga acctcaagac cgccgacccg
     6241 cgcaaggtcg tgacgctcga gctcggcggc aacgcgggcg tgatcgtcca ctccgacgcg
     6301 gacctcgaca aggccgcgca gcggatcgcg ttcggcggct actaccaggc ggggcagtcg
     6361 tgcatctcgg tccagcgcgt gctcgtgcaa gagagcgtgg cggacgacct ctccgagcgt
     6421 ctcgtagcgg aggtcggcaa gctgaagacc ggcgatccgc tcgaccccga gaccgaggtc
     6481 gggccgctga tcgaccacga cgcgctcgag cgcgtgagcg cgtgggtcga cgaggcggtg
     6541 caggcggggg ccgaggtcct ggtcggcggc aagcgagagg acccgttcta cgtaccgacg
     6601 gtgctgagcc gcacgcggcc cgagatgaag gtccggtgcg aggagatctt cggccccgtc
     6661 acgacgatcg gcacctacgg cgacttcgag gaggcgatcg ccgaggtcaa cgagtcgaag
     6721 tacgggctgc aggcaggcgt cttcacctcg gacgtgcaga ggctgttcct cgccttccgc
     6781 gacatagagg tcggcggcgt catcgcgaac gacgtctcgg ccttccgcgc cgaccagatg
     6841 ccgtacggag ggacgaagga ctcgggctcc gggagagagg ggctgcggtg ggcgatgcag
     6901 gagatgaccg agacccgcgt gctcgtgctc tccgacgttc ccctctgagg atcgtctggg
     6961 cgggcccggc ctcgcttctc gggctgctcc tcggggcgcc gttcgagagg cggcgcgtga
     7021 cgcagggcgt cttggtgtgc gaggggtcgt cgtggccgcg ccggctcggc tggaggtacc
     7081 gagcgatcac gttcggccac gtcgtcctgt cggtgggcga cctcgacgac cgcacgctcg
     7141 cgcacgagct cgtgcacgtc cgccagtacg aacggtgggg gatcctgctg tggccggcgt
     7201 acggcctggc ctctgtgtgg gcgaagctgc gcggcggcga cgcatactgg gacaaccact
     7261 tcgagagaca agcgaggggt gaggcatgag acgactgagg ctcgcagcgg cagccgttgt
     7321 ctttgcgacc gtcgccgtac cggggacggc ggccgctccg ttaccggcgg cccccgattg
     7381 ccggatcttc ccggagagca acgtgtggaa ccggcgcgtc gacgacctgc ccgtgcacgc
     7441 tcggtcgcgg gcgatcatcc gcagcatcgg ccgcagcgag ggcctccatc ccgacttcgg
     7501 ctccggccgc tgggacggcg gccccatcgg gatcccgttc acggtcgtcg gcgacggaca
     7561 gcgcaagtcg cgggtgcgct tccagtacgc ggacgagagc gacaaggggc cgtacccgat
     7621 ccccaaggac gtgccgatcg aagggggccg gcagtccgac ggcgaccgtc acgcgctgat
     7681 cctgcagaag gacgagtgcc gcttgtacga gctctacgcg ctgcggcgcc gtgacggccg
     7741 ctggcgcgcg ggctcgggtg cgatctggga cctcgactcg aacgagcttc gcccgaaggg
     7801 atggacctcc gcggacgcgg ccgggctccc gatcctgccc gggctggcgc gctgggacga
     7861 ggtgaagcgc ggcgtcatcg accacgctct gcgtttcacg gtcagccgca cgcgagcgaa
     7921 gtacatctat cccgcgaggc attacgcgag cgactccgac gacgcgaacc tgccgccgat
     7981 ggggctccgg ctgcgcctga agaagagctt ccccgtgaag ggcttcccgc gtcaggcgcg
     8041 catcgtgctc gtcgcgctca agcgctacgg gatgatcgtc gccgacaacg gatcggactg
     8101 gttcatcagc ggtgcgccga acccgcactg ggacaacgac gcgctccgca cgctgggcga
     8161 ggtgaagggc tccgacttcg tcgtcgtcga cacgagcgag ctgcggccct ggaaagactg
     8221 agggcggccc cgccggggag ccgccctcgt cctacgtgtc gttggttatc tgcggtgacg
     8281 gtgcgtcttg gccgctgcct gtgacggtag gcattccgtg ccggtctcgg cgtcggtgta
     8341 ccgagtcgac acgacccggt actggccggt ccggtccggg accttcctgc tgaacttgcc
     8401 gtcgacgtcg gtggccagcg aagcgatcgt cttgaacttg ccgtcgatct tgcgctggat
     8461 gttgaccggc gtcccgacgc cgcacgtgcc gccggcttcg gtgtcgctac cctggccgat
     8521 gacgctcccg ctcacgacga cgtgacgctt cagcgagaag aggatcgtcg gtctgatcgg
     8581 ctctccgccg cagttgggct cacagccacc cggcgccgcc tgccagtcgg cgcggtccgt
     8641 gcccggcccc gccggcgtga gcggagtctc ggtgccgccc ggcgagcccg accggatgta
     8701 gatgcgtccg acggagttca cggcagggct ctgcttcgtg aacgcgatct tcgtcccgtc
     8761 cggtgaccac gcgggctcgt agttgtagtt gtcgtcggac gtgagctggt ccttgcccga
     8821 cccgtcccag ctcatcgtgt ggatctggtc gcggccggcc tcgtcctggc tcgagtaggc
     8881 gagcatgtcg aagctcgggt gccagttcgg cccgaagtcc ggcacgtcgt tgttcgtgag
     8941 gtcggtcttg tccgtcccgc cgggcgtcat cgtccagatg tcggattcgc cctgggcggc
     9001 ggagttctgg aggctcacgt aggcgatcag gtctccgtgg cccgttccgt cgttgcccca
     9061 cgcgggggcc cagtcgtcga cgtcgttgtt cgtcaggttg tacgggccca tgctcgggtc
     9121 ggtgaggtcc cacatgtaga tgtcctggtc gccgtcaccg ttgctgacgt agacgacgta
     9181 cctcccgtcg aacgagaacg tcgggtccgt ctcgatcgcg ggactttccg tgaggttcgt
     9241 gggctccatg ctctcgaggt cgactagcac gagctcgccg ttgctcgagt agacgaggcg
     9301 ttcgcccgcg gcgtccgtgg agaagttcga gaactgcgtc aggttctcgg cgacgctgcc
     9361 cacgacttcg ccggcctggt tgtacctgac gatgctggtc tcgttcccct cgttctcgac
     9421 catgaatatg tcgccgttgc tgcccggcca ggatgccgcc gccggggtcg ag
//
LOCUS       DASQDO010000310         9203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_521786, whole genome shotgun sequence.
ACCESSION   DASQDO010000310 DASQDO010000000
VERSION     DASQDO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521786"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1867
                     /locus_tag="VEV43_08790"
     CDS             101..1867
                     /locus_tag="VEV43_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23656.1"
                     /translation="MPDMWRSAPGTRRAPGSVVKKLAGSFLVGAMSVGLAWAGQGLVP
                     VSDRGEILDRGMEVVQPGVRQAPESGTTLSAETPQLDLSSGQLLAGAAKVSLKPQPEA
                     YGGTWEQSYDKCANLAPNQEGVQEGLTHVADFRVRWAENSNCIFMGGYGIGPMNPIVK
                     WDDEYGLWSRSVAMSDGADTVVLTLIDAVYWEAYYNNMCEGCGFLELADKLAKETGLK
                     PESFVFASTHSHTSPDFIGGWGGVPEWYMTQATESLQASAKQAVASMEPAVLEAGEVI
                     ARQFNAERRDFYRSAEDDGFSWFRLVDADNQPSPEVCTTPAPAATPHPGTKPKPTPTP
                     TPVCTPPNPGKAIATVGAYAAHPVTADESSGTADADFPAVFAKSVEERFGGVGMFLQT
                     GLGNMSPRGNKVEMGNGLSALVPAIGGGQQVANPDVRVGRAFWDQPVTNVPLGSLGVA
                     GFFDRKFNQAPATVAEGKGSAENKKCRSASAVSVRTSVSAAKIGNLWITGGPGELFSN
                     LTNTIEERNPSGVTLALGLVNDGLGYIVQSFETDHVGRQGGGFAGSAGPVGLSEYEDA
                     YSIDHCFGDATLEQTIRLLGTL"
     gene            1871..3343
                     /locus_tag="VEV43_08795"
     CDS             1871..3343
                     /locus_tag="VEV43_08795"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HYP23657.1"
                     /translation="MRRAAPAVAAALVATALAGASGPASAAPTCDWLAGDLHVHTTFS
                     HDSYGGPSDDNTGPEEAYTLGHSVTSQFTVAAARGLDFTAITDHNDVRSQSDAGFGSA
                     GVIGVPGYEASYRGHAQMLGARRVYDGSSATAAGVQALADALRADGGAFQINHPAEGS
                     TDYPHDRDWSYGHSVVPDTVEVWNISRLWQPPLPSGSSNDDAIRFWEEFLDAGHRVAP
                     TGGSDNHYLATTPLQGVGQPTTWVCAENASVAGILGGLRAGRTFVSHQPPAHGGPRLF
                     VEGDADRDGVYESIAGDEIPPGSPLQVRVEGAPGSLLQVIGTGGEPLGDPVPVTGPSF
                     AHELTPDRSVTWVRAEIYEPDAGAERAAVCDGFFGGESTYCRNDLAVLAMTSAIFLAE
                     PFDPATTLTYEGPTSAHAGSKAIFSVALTGSDGPIAGAPVTFSYRDRAFPATTDAFGV
                     ARAATKVAGPPGAYELRADFAGDDRYEPSSWAGTFTVTTP"
     gene            3612..4571
                     /locus_tag="VEV43_08800"
     CDS             3612..4571
                     /locus_tag="VEV43_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23658.1"
                     /translation="MRGTLKNCVVVALVVTGAIGPGVSSAQPADPPGHATRSDLVDVS
                     NAPICKVKPWKGFETAEEAAAIPEDAPVCITPRDMAVTHRAGKGGPVPGPVEPDPNGT
                     NYRYSGVSTQDMYKGIEATIQVSNASVTHDGSGNEFVVSRVMGKSPVSGNWLEAGWAE
                     TSWEGDFPYVYTFTPYNNPNNNWVGYFQYRLSPSLYYKWRVDRCFVGATTVHTCAFIY
                     WGGSWHMLTSGDYACTEYSGGTDNQRCYMEAFTEVFSRDSTPTPSIATGNSLDWDAVK
                     IRRGTTWYLLTTEYPVNVSGSEYPYVVCFQARHYDFSVHRYSC"
     gene            4663..5877
                     /locus_tag="VEV43_08805"
     CDS             4663..5877
                     /locus_tag="VEV43_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23659.1"
                     /translation="MTKQKRVVVRAIVAALAAVGLVLSPAPVTAEIDQSENLKRTRSY
                     KYKGAVYPPVASDDYFKGGTDIAFDGNRIYAMQQGADGGVHIFKRTRTKRGHVKLGFV
                     PCPGEQNDVEVLKPGLVAIGYHSSTCGVTGGGVRLIDVSNPKKPDYLGSVTFEVGSHT
                     LTKYPGKQLIYSSPNGLGDVQGTETIIDVSNPDKPEIVASYANRANSCHDLTFYFDED
                     KKLAFCPGSSRTEIWDVSDPLAPNPIGEIVNPSISYHHSAVATYDGKYLVIGDENTLA
                     DECEGGTTGALWVYDIQDPTAPQPVSYFGIGRGQTPGSAGSDRNSYCTAHLYNFIPGT
                     YVMVASWYAAGMNVIDFSDPANPVEIGHFFGSGDDYRNYWSAYWHQGRIYANDRTRGL
                     DVFRPRKGLIPR"
     gene            6015..6188
                     /locus_tag="VEV43_08810"
     CDS             6015..6188
                     /locus_tag="VEV43_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23660.1"
                     /translation="MSRCFRAIVGAMCLGSMASFTTIAAFSPPPIVVGALLVIGIVGG
                     AVHGSAHDACKEV"
     gene            6190..6375
                     /locus_tag="VEV43_08815"
     CDS             6190..6375
                     /locus_tag="VEV43_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23661.1"
                     /translation="MHGTYISAGVRWSTWGVAFAGALLAHAGWKAAGLTVWLLAVAAQ
                     WVLGIRVLINRRKARGR"
     gene            6413..7384
                     /locus_tag="VEV43_08820"
     CDS             6413..7384
                     /locus_tag="VEV43_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP23662.1"
                     /translation="MSTPRGGIRVEGLRKSFGKVEALTGLDLEVQEGRVLGLLGPNGA
                     GKTTAVRILTTLLAPDAGRAEVAGLDVVTQAEELRSQIGLAGQYAAVDEYLTGFENLE
                     MVGRLYHLPKSIARRRAHELLERFDLVDAGSRVAKTSSGGMRRRLDLAASLVLSPPVM
                     FLDEPTTGLDPRSRLAMWDVIAELVAGGTTVLLTTQYLEEADRLADEIAVIDQGRSIA
                     EGTSDELKAQVGGERLEVTVRNPESFDAAVSILGPFGSEVRPERELRRVSVAITNGAG
                     LLPKVIRELDGAGVELEDLGLRKPTLDDVFLTLTGHTAEAEPGKEGA"
     gene            7381..8199
                     /locus_tag="VEV43_08825"
     CDS             7381..8199
                     /locus_tag="VEV43_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP23663.1"
                     /translation="MSTARLTAEELKPKAGSKLYWAFADAVVLARRHLVQIPRIPELL
                     VFATVQPVMFVILFRYVFGGSIDVGPISYVDYLMAGIFVQTVAFGAVATGIGLAGDLQ
                     MGLIDRFRSLPMSRSAVLTGRTIADLVRNMFVIVIMLVVGMLVGFRPEAGVADWLAAV
                     GLLLLLSFTFSWIGAAVGLLVRTVEAAQSGGFIWLFPLTFTSSAFVQPEKMPGWLQPF
                     AENQPFTVIVNAVRSFFLDAPLGNDGWLAFAWLLGLLFVFAPLSVRLYEKRTAG"
     gene            complement(8196..8891)
                     /locus_tag="VEV43_08830"
     CDS             complement(8196..8891)
                     /locus_tag="VEV43_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23664.1"
                     /translation="MDYSPAPDERVRLRPVNPLTVAVAVVLLSAGGAFGWRMKTGAPS
                     ARPTPEAAAAALALERVDGEPMCGPGGHDEVRDEMLRGGSTGLPERLAEYLPEKSPLP
                     GYDGTSSHAIDPADETEFVSGAVDGHVASFHPAGEGGFDVYAYRFLTRRAAADSVADN
                     VVRRVCEFGANPLAARGHAGMIVVEERGRGDWSSAWWLNESDVLVVRYGGWGDAKTSL
                     ANLAAVAGATASL"
     gene            8988..>9203
                     /locus_tag="VEV43_08835"
     CDS             8988..>9203
                     /locus_tag="VEV43_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23665.1"
                     /translation="MGTARRTTVALLSAALLIGGVAPAVARPTAQGPARKPKLPRPID
                     PQSWEVPEDMTWGDYRRIPGTDWMNPEY"
BASE COUNT         1432 a         3288 c         3140 g         1343 t
ORIGIN      
        1 cccggaaagg tttactccgc cgggcgggct cggcccgtgg aggaagtcgg gcgcccgggg
       61 cgaactggcc actagtcccc gcctaggagg tcctttctcg atgcccgaca tgtggaggag
      121 cgcccccgga acgcgccgcg ctcccggatc cgtcgtcaag aagctcgccg gctcgttcct
      181 cgtcggtgcg atgtccgtcg gcctcgcgtg ggccggccag ggcctcgtcc cggtctcgga
      241 ccgcggcgag atcctcgacc ggggcatgga ggtcgtccag cccggtgtcc gccaggcccc
      301 cgagagcggc acgaccctca gcgcagagac cccccagctc gacctctcgt ccgggcagct
      361 cctcgcgggg gccgcgaagg tctcgctgaa gcctcagccg gaggcctacg gcggcacctg
      421 ggagcagagc tacgacaagt gcgccaacct cgcgccgaac caggagggcg tgcaggaagg
      481 gctcacgcac gtcgccgact tccgcgtgcg ctgggccgag aactcgaact gcatcttcat
      541 gggcggctac ggcatcggcc cgatgaaccc gatcgtgaag tgggacgacg agtacggcct
      601 gtggtcgcgc tcggtcgcca tgagcgacgg cgccgacacc gtcgtcctga cgctgatcga
      661 cgccgtgtac tgggaggcct actacaacaa catgtgcgag ggctgcggct tcctcgagct
      721 ggccgacaag ctggcgaagg agaccggcct gaagcccgag agcttcgtct tcgcctcgac
      781 gcacagccac acctcgccgg acttcatcgg cggctggggc ggcgtccccg agtggtacat
      841 gacgcaggcg accgagtcgc tccaggcttc cgccaagcag gcggtcgcgt cgatggagcc
      901 ggcggtcctc gaagccggcg aggtcatcgc gcgtcagttc aacgccgagc gccgcgactt
      961 ctaccgctcg gccgaggacg acggcttctc gtggttccgc ctcgtcgacg cggacaacca
     1021 gccgagcccg gaggtctgca cgacgccggc tccggccgcg acgcctcacc ccggcacgaa
     1081 gcccaagccc acgccgacgc cgacgcccgt ctgcacgcct ccgaaccccg ggaaggcgat
     1141 cgcgaccgtc ggcgcctacg cggcgcaccc ggtcacggcc gacgagtcga gcggcaccgc
     1201 cgacgcggac ttccccgcgg tgttcgccaa gagcgtcgag gagcgcttcg gcggcgtcgg
     1261 gatgttcctc cagacgggtc tcggcaacat gtcgccccgc ggcaacaagg tcgagatggg
     1321 caacggcctc tcggccctcg tcccggcgat cggcggcggc cagcaggtag cgaaccccga
     1381 cgtccgcgtc gggcgcgcgt tctgggacca gccggtgacg aacgtcccgc tgggctcgct
     1441 cggcgtcgcc ggcttcttcg accgcaagtt caaccaggcc cctgcgacgg tggccgaggg
     1501 caagggcagc gccgagaaca agaagtgccg ctccgcctcg gcggtctccg tgcggacgtc
     1561 ggtcagcgcg gccaagatcg ggaacctgtg gatcaccggc ggtcccggtg agctgttctc
     1621 gaacctcacg aacacgatcg aggagcgcaa cccgagcggc gtgacgctgg ccctcgggct
     1681 cgtgaacgac ggcctcggct acatcgtcca gagcttcgag accgaccacg tcggacggca
     1741 gggcggcggc ttcgccggca gcgccggccc ggtcgggctc tccgagtacg aggacgccta
     1801 ctcgatcgac cactgcttcg gcgacgcgac gctcgagcaa acgatccggc tcctcggcac
     1861 gctctagacg atgcggcgcg cggcccccgc ggttgcagca gctctcgtag cgacggcgct
     1921 cgccggtgct tccggcccgg cgagcgccgc gcctacgtgc gactggctcg cgggcgacct
     1981 ccacgtccac acgacgttct cgcacgactc ctacggcggg ccgtcggacg acaacacggg
     2041 gccggaggag gcgtacacgc tcgggcactc ggtgacgagc cagttcaccg tcgcggctgc
     2101 gcgcggcctc gacttcaccg cgatcaccga ccacaacgac gtgcggtctc agtcggacgc
     2161 cgggttcggc agcgccggag tgatcggcgt ccccggctac gaggcctcgt accgcggcca
     2221 cgcgcagatg ctcggggcgc ggcgggtgta cgacggatcg agcgcgacgg ccgccggcgt
     2281 gcaggctctc gcggacgcgc tgcgtgccga cggcggagcg ttccagatca accatcccgc
     2341 cgagggctcg accgactacc cgcacgaccg cgattggtcc tacggacact cggtcgtccc
     2401 cgacaccgtc gaggtgtgga acatctcgcg gctgtggcag ccgccgctgc cgtccgggtc
     2461 gtcgaacgac gacgcgatcc ggttctggga ggagttcctc gacgcgggcc accgcgtcgc
     2521 cccgaccggc gggagcgaca accactacct cgcgaccacg cccttgcagg gagtcggtca
     2581 gccgacgacg tgggtgtgcg cggaaaacgc gagcgtcgcg gggatcctcg gaggtctgcg
     2641 cgcgggccgg acgttcgtgt cgcaccaacc tcccgcgcac ggggggccgc gcttgttcgt
     2701 cgagggcgac gcggaccgcg acggcgtcta cgagtcgatc gccggcgacg agatcccgcc
     2761 gggctcgccc ctgcaagtgc gcgtcgaggg cgcgccgggc tcgctcctcc aggtgatcgg
     2821 caccgggggc gaacccctcg gcgaccccgt ccccgtcacc ggaccgtcgt tcgcgcacga
     2881 gctgacgccg gaccgttcgg tcacgtgggt ccgggccgag atctacgagc ccgatgcggg
     2941 ggccgagcgc gccgcagtct gcgacggctt cttcgggggc gagtcgacgt actgccgcaa
     3001 cgacctcgcg gtacttgcga tgacgtcggc gatcttcctc gccgagccgt tcgacccggc
     3061 gacgacgctg acctacgagg gcccgaccag cgcgcacgct ggctcgaagg cgatcttctc
     3121 ggtggctctg accgggtccg acggaccgat agccggagcg ccggtgacgt tctcgtacag
     3181 ggaccgggcg ttcccggcga cgacggatgc gttcggggtg gcccgcgccg cgacgaaggt
     3241 ggcgggaccg ccgggggcct acgagctgag ggccgacttc gccggcgacg accgctacga
     3301 gccgtcgagc tgggccggga ccttcaccgt cacgacgccc tgaggcacgt cgccggggca
     3361 cgccacctct agtccgctcg cgctacgcgt caccgcttcg gggctcgacc tgacgcggtg
     3421 tcgccgcctg gggtgtgatc ccgcgcgcga gcctggtccc gcgctgacgc cggcgtgaca
     3481 ctcgaccgac cgatggcgcg atagcccgtt cgtgcggccg aaccatcccg cgtagccatg
     3541 gcttcgaagg cgcgccgtgc ctaccgtcga ctgacgcagc agcgcggtag agcgcggacg
     3601 aaaggaacgg gatgaggggg actctgaaga attgcgtcgt ggtggcattg gtcgtcaccg
     3661 gtgcgatcgg gcccggcgtg tccagtgccc aaccggcgga cccaccgggt catgccacga
     3721 ggtccgacct cgtcgacgtg tcgaacgcgc cgatctgcaa ggtcaaacct tggaaggggt
     3781 tcgaaacggc cgaggaagca gcggcgatac ccgaggacgc gccggtatgc atcacgccgc
     3841 gggacatggc ggtcacacac cgcgccggaa aaggcggccc ggtgccgggc ccggtcgagc
     3901 ccgacccgaa cggcacgaac taccgctaca gcggggtgtc gacacaggac atgtacaagg
     3961 gcatcgaggc gacgatccag gtcagcaacg cgtcggtcac ccacgacggc agcgggaacg
     4021 agttcgtcgt gtcgcgggtc atgggcaagt cgcccgtgtc cggcaactgg ctcgaggccg
     4081 gatgggccga gacgagctgg gagggcgact tcccgtacgt ctacacgttc accccgtaca
     4141 acaacccgaa caacaattgg gtcgggtact tccagtacag gctctctccc agcctctact
     4201 acaagtggcg ggtcgaccgc tgcttcgtgg gcgcgaccac ggtccacacg tgcgccttca
     4261 tctactgggg aggcagctgg cacatgctga cgtcgggtga ctacgcctgc acggagtatt
     4321 ccggcgggac cgacaaccag cggtgctaca tggaggcgtt caccgaggtc ttctcccggg
     4381 acagcacgcc gacgccgtcg atcgcgaccg gcaacagcct cgactgggac gcggtgaaga
     4441 tcaggagggg aacgacctgg tatctgctca cgaccgagta ccccgtcaac gtgagcgggt
     4501 cggagtatcc atacgtggtg tgcttccaag cccgccacta cgacttcagc gtccatcgat
     4561 atagctgctg agctgtcatc caagcgtgag aggcggccct cgacgggtcg cctctcactt
     4621 tttctagggt tgaaggagat cccggctggg gagcatgaag acgtgaccaa gcagaagaga
     4681 gtggtcgtcc gggcgatcgt ggcggctctg gcggccgtcg ggctcgtcct gtccccggcc
     4741 cccgtgacgg ccgagatcga ccagtccgag aacctcaagc gaacccggtc ctacaagtac
     4801 aagggcgccg tctacccgcc cgtcgcctcc gacgactact tcaagggcgg caccgacatc
     4861 gctttcgacg gcaaccgcat ctacgcgatg cagcaggggg ccgacggcgg cgtgcacatc
     4921 ttcaagagga cgcgcacgaa gcgcgggcac gtaaagctcg gcttcgtccc ctgcccgggc
     4981 gagcagaacg acgtcgaggt cctcaagccg gggctggtcg cgatcgggta ccactcgtcg
     5041 acctgcggcg tcaccggcgg cggggtcagg ctgatcgacg tctcgaaccc gaagaagccc
     5101 gactacctcg ggagcgtgac gttcgaggtc ggctcgcaca cgctcacgaa gtaccccggc
     5161 aagcagctga tctactcgtc gccgaacggg ctcggcgacg tccaggggac cgagacgatc
     5221 atcgacgtgt cgaacccgga caagcccgag atcgtggcga gctacgcgaa ccgcgcgaac
     5281 tcctgccacg acctcacgtt ctacttcgac gaggacaaga agctggcgtt ctgtccgggc
     5341 tcgtcgcgca ccgagatctg ggacgtctcc gatccgcttg cgcccaaccc catcggcgag
     5401 atcgtgaacc cgtcgatctc ctatcaccac tccgccgtgg cgacctacga cgggaagtac
     5461 ctcgtgatcg gggacgagaa caccttggcg gacgagtgcg agggcggcac gacgggcgcg
     5521 ctgtgggtct acgacatcca agacccgacg gcgccccagc ccgtcagcta cttcggcatc
     5581 ggccgcggcc agacccccgg gagcgcgggc tccgacagga acagctactg caccgcgcac
     5641 ctctacaact tcatccccgg cacgtacgtg atggtcgcgt cctggtacgc ggcgggcatg
     5701 aacgtcatcg acttctccga cccggcgaac ccggtcgaga tcggccactt cttcggctct
     5761 ggcgacgact accggaacta ctggtcggcg tactggcacc agggccggat ctacgcgaac
     5821 gaccgcacgc ggggcctgga cgtgttccgg ccgcgcaagg gcctcatccc ccgctaggcg
     5881 ttccccgccc cgcgtgcgca agtagacgcg gaaagcgcca cgcgttgcgc acgcgccggg
     5941 tgtgacgcgc gccattgtcc gacccccttc gcagacctac cttcgggacc catcccgaag
     6001 gagatcgcgc acaaatgagc aggtgtttcc gtgccatcgt gggggcgatg tgcttaggat
     6061 cgatggcgtc gttcacgacg atcgccgcat tctcgccgcc gccgatcgtt gtcggcgcgc
     6121 tgttggtcat cggcatcgta ggcggagcgg tgcacggttc cgcacacgac gcgtgcaagg
     6181 aggtgtgacg tgcacgggac gtacatctcg gccggtgtcc ggtggtcgac gtggggtgtc
     6241 gcgttcgcgg gcgcgcttct tgcgcacgcc ggctggaaag cggccggcct caccgtttgg
     6301 ctcctcgccg tcgccgccca gtgggtcctg gggatccgcg tcctgatcaa ccgcaggaag
     6361 gcgcgaggcc ggtaacgggg cccccgggct cgctctgtag agtgctccgg ccatgagcac
     6421 tccacgaggc ggtatccggg tcgaagggct ccgcaagtcg ttcggcaagg tcgaggccct
     6481 caccgggctc gacctcgagg tccaggaggg ccgcgtcctc ggcctcctcg ggcccaacgg
     6541 ggccggcaag acgaccgccg tccgtatcct caccacgctc ctcgcgcccg acgcgggccg
     6601 ggccgaagtc gccgggctcg acgtcgtcac gcaggccgag gagctccgct cgcagatcgg
     6661 cctcgccggc cagtacgccg ccgtcgacga gtacctcacc gggttcgaga acctcgagat
     6721 ggtcggccgg ctctaccacc tgccgaagtc gatcgcccgg cggcgcgcgc acgagctcct
     6781 ggaacgcttc gacctcgtcg acgccgggtc gcgcgtcgcc aagacgtcct cgggcggcat
     6841 gcggcggcgc ctcgacctcg cggcgagcct cgtgctctct cccccggtca tgttcctcga
     6901 cgagccgacc acgggcctcg acccgcgcag ccggctcgcg atgtgggacg tcatcgccga
     6961 gcttgtcgcc ggcggcacga cggtgctcct cacgacgcag tacctcgagg aggccgaccg
     7021 cctcgccgac gagatcgcgg tcatcgacca gggccgctcg atcgccgaag gcacgtcgga
     7081 cgagctgaag gcgcaggtcg gcggcgagcg gctcgaggtc accgtccgca accccgagag
     7141 cttcgacgcc gcggtctcga tcctcggccc cttcggctcc gaggtgcgcc cggaacgcga
     7201 gctccgccgc gtctccgtcg cgatcacgaa cggtgccggg ctgctcccga aggtgatccg
     7261 cgagctcgac ggggccggag tcgagctcga agacctcgga ctccgcaagc cgacgctcga
     7321 cgacgtcttc ctcacgctga ccgggcacac cgccgaagcc gagcccggga aggagggggc
     7381 atgagcacag cgcggctcac cgcggaggag ctgaagccca aagccgggag caagctctac
     7441 tgggccttcg ccgacgcagt cgtcctcgcg cgccggcacc tcgtccagat cccccggatc
     7501 cccgagctgc tcgtgttcgc gaccgtgcag ccggtcatgt tcgtgatcct gttccgctac
     7561 gtgttcggcg gatcgatcga cgtcggcccg atcagctacg tcgactacct catggcgggg
     7621 atcttcgtgc agaccgtcgc gttcggcgcc gtcgcgaccg ggatcggcct cgccggagac
     7681 ctgcagatgg gattgatcga ccggttccgc tcactgccga tgtcgcgttc tgccgtgctc
     7741 acgggccgga ccatcgccga cctcgtgcgg aacatgttcg tgatcgtgat catgctggta
     7801 gtcggcatgc tcgtcgggtt ccgtccggaa gcgggcgtcg ccgactggct cgcggcggtc
     7861 ggcctcctgc tgctgctcag cttcaccttc tcgtggatcg gtgcggccgt gggcctcctc
     7921 gtgcgcaccg tcgaggcggc ccagtccggc gggttcatct ggctgttccc cctgacgttc
     7981 accagcagcg cgttcgtcca gcccgagaag atgccgggct ggctgcagcc cttcgccgag
     8041 aaccagccgt tcaccgtgat cgtcaacgcg gtgcggagct tcttcctcga cgcgccgctc
     8101 ggcaacgacg gatggctcgc gttcgcgtgg ctgctgggcc tcctgttcgt cttcgcgccg
     8161 ctgtctgttc ggctgtacga gaagaggacc gcgggctaga gggacgcggt cgcgccggct
     8221 accgccgcca ggttcgccag gctcgtcttc gcgtcgcccc agcccccgta gcgcacgacg
     8281 agcacgtcgg actcgttgag ccaccacgca ctcgaccagt ctccccggcc ccgttcctcg
     8341 accacgatca tcccggcgtg gccgcgagcg gcgagcgggt tggccccgaa ctcacacacc
     8401 cttcggacga cgttgtcggc gacggagtcg gccgcggcgc ggcgcgtgag gaagcggtac
     8461 gcgtagacgt cgaagccgcc ctcccccgcc ggatggaacg acgcgacgtg tccgtcgacc
     8521 gcgccggaga cgaactcggt ctcgtccgcg gggtcgatcg catgcgacga cgtcccgtcg
     8581 taaccgggca gcggcgactt ctcggggagg tactcggcca gtcgctccgg gagcccggtg
     8641 gaccccccgc gcagcatctc gtcgcgcacc tcgtcgtggc caccgggccc gcacatcggc
     8701 tcgccgtcca cccgctcgag tgccagagcg gccgcggccg cctccggcgt gggccgcgcc
     8761 gacggagcac ccgtcttcat ccgccagccg aacgcgccgc cggccgacag cagcaccacg
     8821 gcgacagcga ccgtcaacgg gttcaccggc cggagccgga cgcgctcatc gggcgcgggc
     8881 gagtagtcca cctcctccca tcggacggcc gggcttgctt cctttagcag cccaggcgaa
     8941 gtgcgcatat cgtcgcgggg cgatcgtcga ctgcaccgga ggccgggatg ggaaccgcac
     9001 gccgtacgac cgtggcgctg ctcagcgccg cgttgctcat cggaggcgtg gcgcccgccg
     9061 tcgcccgtcc caccgcacag ggcccggccc ggaagccgaa gctcccccgc ccgatcgacc
     9121 cgcagtcgtg ggaggtcccg gaggacatga cctggggcga ctaccggcgg atccccggga
     9181 cggactggat gaaccccgag tac
//
LOCUS       DASQDO010000311         4437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_277648, whole genome shotgun sequence.
ACCESSION   DASQDO010000311 DASQDO010000000
VERSION     DASQDO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277648"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VEV43_08840"
     CDS             complement(<1..210)
                     /locus_tag="VEV43_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYP23666.1"
                     /translation="MAKRIRYVVVAAATTALLAAPATAHDRVWKGEAIYYSDEFAGET
                     MACGGKYRPRKMVAAHRKLPCGTTIR"
     gene            300..905
                     /locus_tag="VEV43_08845"
     CDS             300..905
                     /locus_tag="VEV43_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23667.1"
                     /translation="MKKTLVSLALCAAVAASAVPAHAAKGGLKTLGTDPSGDGLPGLD
                     VTYLQVGQTGKDLEVRIGLDMLPAAAGYPDLPGIEWVFDVQPPMPRCPRNHMCLAVMP
                     PARTFVAEAVKTADGSAYYLFEVHDDGSFEQISQPQGTYDAADGYASILVPLKDIGAT
                     KGATIKGTDGLEHGDVDAHVHLGTVTHYPDGMETDKGYTVR"
     gene            945..2525
                     /locus_tag="VEV43_08850"
     CDS             945..2525
                     /locus_tag="VEV43_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYP23668.1"
                     /translation="MRLLRLGLTLAVCAALLAPVAPATAQPAPRNYPQFTYERVRVPM
                     RDGVELSADVYRPVTPEGVKVPVILQLTPYHVLYKALDNNETNLPSGDAEFLVPKGYA
                     YVVADVRGTYNSGGCWDYGGIKEREDGYDIVEWLGTRPWSNGKVAMTGASYDGTTANA
                     AAVEQPPHLATIVPVSAISRWWGYAYQQGARSTYSGESADIDPPSDTPTDFMFAYGFV
                     PPPDPGALGHAEQIAMRWNLCDRVEKALKGYDTEPDYDEFWEERDYLRLAHKVKVPVL
                     VSHGQLDFNVKTWEGTSWFDAVKTEKAMVLGQWAHASPRGRYREWDTFLLKWFERWLY
                     GVRNGVEKEPAVRVQTNDGQWHLQNEWGPEKTAKLKLSGGEATYFDDGALTESEMTRG
                     LSADRYVRVEVPKSSGVRIEGRPVLHLEASSDQASTHFVAVLCDVGPSGGCGVISRAF
                     MNARYRETLKEGKDLEPGKRYGFDLEFIDKDYVVADDHHLELIIASSSNTWVAPDERR
                     ANNTLFLDGSYLKLPLSR"
     gene            2539..3408
                     /locus_tag="VEV43_08855"
     CDS             2539..3408
                     /locus_tag="VEV43_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP23669.1"
                     /translation="MTTPAWLPRELYPFESRYADLGDCTVHYVDEGSGLTILFLHGNP
                     TWSFLYRNVIRGLRDSFRCIALDYPGFGLSWVRPGSGYGYRVADHADAVERFVLELDL
                     RDVVLTAQDWGGPIGLTVATRHPDRFRGFVLGNTWAWPVNGTFHFEAFSRVMGGPVGR
                     AFIRHANAFVNVMIPLGTATKLPDEVMAAYRGPFQTPGERRPTWELPREILRARPFLQ
                     RLLDSMPVLADRPALFVWGGGDTALRSSVELPRLQELFPDHETVVLEGAKHYFQEDAP
                     EEVTAAIRAWMNR"
     gene            complement(3405..3644)
                     /locus_tag="VEV43_08860"
     CDS             complement(3405..3644)
                     /locus_tag="VEV43_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23670.1"
                     /translation="MVVALVGSLTWWIYGTPFDGWTRADERHVKEVCIERGGGTEKFC
                     NCFVEELQQEGVAFEDVNSQNGRKAGFVCGAFFQF"
     gene            complement(3740..>4437)
                     /locus_tag="VEV43_08865"
     CDS             complement(3740..>4437)
                     /locus_tag="VEV43_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23671.1"
                     /translation="GGFFMVDTSDFAKGVAKPKVKPLTPIQNRVSWGDPGVHSAVPFP
                     GRDYVWTTDEVYGEVPVLLAGHGCPWGWSRVVDVRNPKKPKVIAEYRLPQNTEEFCAS
                     QTDNDPSRNALSSWAAHNPTLTQNLAILTWHSGGLQAVDISKPGAPAQAAEFKPEPLP
                     AVVQEDPVLSSGRDKVVAWSYPVIKDGLIYFSDVRNGLYIVRYEGPFENEVSSLDFLE
                     GNSNLGDALGIFK"
BASE COUNT          703 a         1566 c         1505 g          663 t
ORIGIN      
        1 ccggatcgtg gtaccgcagg ggagcttgcg gtgcgcggcc accatcttgc ggggccggta
       61 cttcccgccg caggccatcg tctcgccggc gaactcgtcg gagtagtaga tcgcctcgcc
      121 cttccagacg cggtcgtgag cggtcgccgg cgcggcgagc agcgcggtcg ttgccgccgc
      181 gaccaccacg tatcggatcc tcttcgccac cgcgttcctc ccgcactaaa aagagcccgt
      241 ccaagggctc ggcggggacc ctagcaggag ccgcgggggc gccggagaac tacccgtcca
      301 tgaagaagac gctcgtctcg ctcgccctct gtgccgccgt ggcagcttcc gccgtgcccg
      361 cgcacgccgc caagggcggc ctcaagacgc tcggcaccga cccctccggg gacggtctgc
      421 ccggcctcga cgtcacctac ctccaggtcg gccagaccgg caaggacctc gaggtccgga
      481 tcggcctcga catgctgccg gcggccgccg gctaccccga cctgccgggg atcgagtggg
      541 tcttcgacgt ccagcctccg atgccgcgct gcccccgcaa ccacatgtgc ctcgccgtca
      601 tgccgccggc ccggacgttc gtcgccgagg ccgtgaagac cgccgacggc tccgcctact
      661 acctcttcga ggtgcacgac gacggcagct tcgagcagat ctcgcagccg cagggcacct
      721 acgacgcggc cgacggctac gcgagcatcc tcgtccccct gaaggacatc ggcgccacga
      781 agggcgccac gatcaagggg accgacgggc tcgagcacgg cgacgtggac gcccacgtgc
      841 acctcgggac cgtcacccac taccccgacg gcatggagac ggacaagggc tacacggtcc
      901 gctagaacag aggtcccgcg ccacccgcgt cgaaccccat cctcatgcgt ctcctccggc
      961 taggcctcac gctcgcggtc tgtgccgcgc tgctcgcgcc ggtggctccg gcgaccgccc
     1021 agccggcccc ccgcaactac ccgcagttca cgtacgagcg agtccgcgtg ccgatgcgcg
     1081 acggggtcga gctctccgcg gacgtctacc gcccggtcac gcccgagggc gtcaaggtgc
     1141 cggtcatcct ccagctcacg ccgtatcacg tgctctacaa ggccctcgac aacaacgaga
     1201 cgaacctgcc gtccggcgac gccgagttct tagtgccgaa gggctacgcg tacgtcgtcg
     1261 ccgacgtgcg tgggacctac aactcgggcg ggtgttggga ctacggcggc atcaaggagc
     1321 gcgaggacgg ctacgacatc gtcgagtggc tcggcacgcg gccgtggtcg aacggcaagg
     1381 tcgcgatgac cggagcgtcc tacgacggga cgacggcgaa cgctgccgcg gtcgaacagc
     1441 ccccgcacct cgcgacgatc gtgccggtct cggcgatcag ccggtggtgg ggctacgcct
     1501 accagcaggg ggcccggtcg acgtactccg gcgagtccgc ggacatcgac ccgccgtccg
     1561 acaccccgac cgacttcatg ttcgcctacg gcttcgtccc gccgcccgac ccgggcgcgc
     1621 tcggacacgc cgagcagatc gcgatgcgct ggaacctctg cgaccgcgtc gagaaggcgc
     1681 tgaagggcta cgacaccgag cccgactacg acgagttctg ggaggagcgg gactacctcc
     1741 ggctcgcgca caaggtgaag gtcccggtcc tcgtgtcgca cggccagctc gacttcaacg
     1801 tgaagacgtg ggagggcacg tcgtggttcg acgcggtgaa gaccgagaag gcgatggtgc
     1861 tcgggcagtg ggcgcacgcg tcgccccgcg gccgctaccg cgagtgggac acgttcctgc
     1921 tgaagtggtt cgagcgctgg ctctacggcg tccgcaacgg ggtcgagaag gagcccgccg
     1981 tgcgcgtgca gacgaacgat ggccaatggc acctgcagaa cgaatggggg ccggagaaga
     2041 ccgcgaagct caagctctcc gggggcgagg ccacttactt cgacgacggc gcgctgacgg
     2101 agtccgagat gacgcggggg ctgtcggcag accgctacgt acgggtcgag gtgccgaagt
     2161 cgtcgggggt ccggatcgag ggccgtccgg tgctccacct cgaggcgtcg agcgaccagg
     2221 cgagcacgca tttcgtcgcg gtgctctgcg acgtcggccc ttccggcggc tgcggggtca
     2281 tctcccgggc gttcatgaac gcgcgctacc gcgagacgct gaaggagggg aaggacctcg
     2341 agcccggcaa gcgctacggg ttcgacctcg agttcatcga caaggactac gtcgtcgccg
     2401 acgaccacca cctcgagctc atcatcgcct cgtcgagcaa cacctgggtg gcccccgacg
     2461 agcgccgcgc gaacaacacg ctgttcctcg acggtagcta cctgaagctg ccgctcagcc
     2521 gctagggact acggctcgat gaccacgccg gcgtggctgc cgcgggagct ctatcccttc
     2581 gagagccgct acgccgacct cggcgactgc accgtgcact acgtcgacga gggctcgggt
     2641 ctcacgatcc tcttcctgca cgggaacccg acgtggtcgt tcctctacag gaacgtgatc
     2701 cgcggcctga gggactcgtt ccgttgcatc gcgctcgact accccgggtt cggactgtcg
     2761 tgggtgcgac ccgggtcggg ctacggctac cgagtcgcgg accatgcgga cgcggtcgaa
     2821 cgcttcgtgc tcgagctcga cctccgagac gtcgtcctga cggcgcagga ttggggcggc
     2881 cccatcgggt tgaccgtcgc gacgcgacac ccggaccgct tccgcgggtt cgtcctcggc
     2941 aacacgtggg cgtggccggt caacggcacg ttccacttcg aggcgttctc gcgcgtgatg
     3001 gggggcccgg tcggaagggc cttcatccgg cacgcgaacg cgttcgtcaa cgtgatgatc
     3061 ccgctgggga cggcgacgaa gctgccggac gaggtcatgg cggcgtaccg ggggccgttc
     3121 cagacgccgg gcgagcgccg accgacgtgg gagctgccgc gcgagatcct gcgcgcgcgg
     3181 cccttcctcc agcgactgct cgacagcatg ccggttctcg cagaccgccc cgcgctgttc
     3241 gtgtggggag gcggcgacac cgcgctacgc agcagcgtgg agctgccgcg cttgcaggag
     3301 ctgttcccgg accacgagac ggtcgtgctg gagggcgcga agcactactt ccaggaggac
     3361 gcgccggagg aggtcaccgc cgcaatccgg gcctggatga accgctagaa ctggaagaac
     3421 gcgccgcaga cgaagccagc cttgcggccg ttctgggagt tgacgtcctc gaacgcgacg
     3481 ccctcttgct ggagctcttc cacgaagcag ttgcagaact tctcggtccc gcctccgcgc
     3541 tcgatgcaga cctctttcac gtgtcgctcg tcggcgcggg tccagccgtc gaacggcgtg
     3601 ccgtagatcc accacgtcag gctcccgacg agcgcgacga ccaccaggcc cgcgatcagc
     3661 gcgaccgatc cgcagcaccc gaagaagcgc cgccgccgcg gctgggactg ctcctgctcg
     3721 tccgccagag gaggctctcc tacttgaaga tgccgagcgc gtcgccgagg ttcgagttgc
     3781 cctcgaggaa gtcgaggctc gacacctcgt tctcgaacgg tccctcgtag cgcacgatgt
     3841 agaggccgtt gcggacgtcg gagaagtaga tcagcccgtc cttgatcacc gggtagctcc
     3901 aggcgacgac cttgtcacgg ccggacgaca ggaccgggtc ctcctgcacg accgccggca
     3961 gcggctcggg cttgaactcg gcggcctgcg ccggtgcgcc cggcttcgag atgtcgacgg
     4021 cctgcagccc tcccgagtgc cacgtgagga tcgcgaggtt ctgagtcagc gtcgggttgt
     4081 gggcggccca gctcgacaga gcgttgcggc tggggtcgtt gtcggtctgc gaggcacaga
     4141 actcctcggt gttctgcggg agccggtact ccgcgatgac cttgggcttc ttcgggttgc
     4201 gcacgtcgac gacgcgcgac cagccccacg ggcagccgtg ccccgctagc aggaccggga
     4261 cctcgccgta gacctcgtcg gtcgtccaga cgtagtcgcg gcccgggaac ggcaccgcgc
     4321 tgtggacgcc ggggtcaccc cagctcacgc ggttctggat cggggtgagc ggcttcacct
     4381 tcggcttcgc cacgcccttc gcgaagtccg acgtgtcgac catgaagaag ccgccgc
//
LOCUS       DASQDO010000312         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_950670, whole genome shotgun sequence.
ACCESSION   DASQDO010000312 DASQDO010000000
VERSION     DASQDO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950670"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2510)
                     /gene="glgP"
                     /locus_tag="VEV43_08870"
     CDS             complement(<1..2510)
                     /gene="glgP"
                     /locus_tag="VEV43_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYP23672.1"
                     /translation="MRALRSFAVRVSLPDKLGPLTELAMNLRWSWNRRARDLWRWVDS
                     EAWDASGHDPVRMLGLVSQERFEQLAEDGPFLSFLAGVSEDLQSYLEEDRWYQSRKKN
                     GPRTIAYFSPEFGVSEALPIYSGGLGVLAGDHLKAASDLGVPLVGVGLLYRQGYFRQY
                     LNADGWQQERYPSLDPHAMPLTLLPGPDGSPLKIEVDLAGARCVAQLWVARVGRVKLL
                     LMDSDVEENDAEERGVTDRLYAGGSEHRLRQEILLGIGGVRALQVAGYEPDVFHSNEG
                     HAGFLGLERIRQIVTAEGMSFDDALEGVRAGTIFTTHTPVPAGIDVYEYDLMQRYFAS
                     FAKECDITFEELMALGQGSSAGGTKSSENGTFNMAVMGLRLAARANAVSKLHGVVSRA
                     IFSDLWPGIPSEEGPITSITNGVHTATWLGPEISEVIDRRLAPGWTETGGAKWQRMEE
                     VPDAELWRARERARERLVYFVRERLRRQLLARGASEAEVGWTDEVFDPGILTIGFARR
                     FAQYKRATLILSDRERLRALLLSGDRPVQLVFAGKAHPQDDGGKEMIRELVHFSQDPE
                     IRGRFAFIEDYDVEVARVLCQGSDVWLNNPRRPLEASGTSGMKAALNGALNCSVLDGW
                     WDECYDGKNGWSIGTADLYEDHVYQDRVDSSSLYDLLEREIAPRFYDRPEGPVPRRWV
                     ERMKSSMSSLGGFVTADRMVKDYVEKLYIPAATHGRELMADRCARARALSEWKKRTRD
                     AWDSVSILNVEGDVTAADVGEQRSVSVTVRLGELSTDDVAVQHAHGRVGANGELVEPD
                     VVELAPADSTDGTCMYTGAFRTDDPGLYGYAVRVIPSH"
     gene            2585..2851
                     /locus_tag="VEV43_08875"
     CDS             2585..2851
                     /locus_tag="VEV43_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYP23673.1"
                     /translation="MAKIVPFTEARSNLTELLDELEARHEHVLITRNGRPSAVLLSAD
                     EFQALEETLDILQDDDLMRALARSERDVEEGDLVPLEELRRPRA"
     gene            2851..3129
                     /locus_tag="VEV43_08880"
     CDS             2851..3129
                     /locus_tag="VEV43_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYP23674.1"
                     /translation="MARVLLTSEARRHFDLLPATFQDAVLEALTAIEVDPEGAGKPLL
                     GRLKGLWSSRVGNYRILYTIEGGRRSPTVIVRAIRHRAVAYEGKRRPR"
     gene            complement(3132..>3732)
                     /locus_tag="VEV43_08885"
     CDS             complement(3132..>3732)
                     /locus_tag="VEV43_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23675.1"
                     /translation="AAPVDAAPAPRTAKLYVHIGETASGVAGEFDFYMDARDYPDAAN
                     RAGNLTNGLPNEAAGAAGGGWRNVFPAKELLPLRVDARRPVTGRIAVGTYEPNVFVAT
                     PAGVGQVVVDLELVGTPAGAGKSVVLGKQTAEFLVTPAAPLHVVEVVIEPEASLHGTT
                     FSEIALVTNFRGASLLTGFFELDDPASYLEIPTLRPHRT"
BASE COUNT          564 a         1308 c         1293 g          567 t
ORIGIN      
        1 tggtgcgacg ggatcaccct gaccgcgtag ccgtacaggc cggggtcgtc ggtgcggaag
       61 gcgcccgtgt acatgcacgt gccgtcggtc gagtcggcgg gcgccagctc gacgacgtcg
      121 ggctcgacca gctcgccgtt cgcgcccacc cggccgtgcg cgtgctggac cgcgacgtcg
      181 tcggtcgaca gctcgccgag ccggacggtg acggagaccg agcgttgctc gccgacgtcg
      241 gctgcggtga cgtcgccctc gacgttcagg atcgacacgg aatcccatgc gtcccgcgtg
      301 cgcttcttcc actccgacag agcgcgggcc cgggcacagc ggtcggccat gagctcgcgg
      361 ccgtgggtcg cggcggggat gtagagcttc tcgacgtagt ccttcaccat gcggtccgcg
      421 gtgacgaaac cgccgaggct cgacatcgac gacttcatcc gttcgaccca tcgccggggc
      481 accgggccct ccgggcggtc gtagaaccgc ggtgcgatct cgcgctcgag gaggtcgtag
      541 aggctgctcg agtccacgcg gtcctggtac acgtggtcct cgtacaggtc ggcggtgccg
      601 atcgaccagc cgttcttgcc gtcgtagcac tcgtcccacc agccgtcgag gaccgagcag
      661 ttcagcgcac cgttcagcgc ggccttcatc ccgctcgtac cgctcgcctc cagaggccgc
      721 cgcgggttgt tcagccacac gtccgagccc tggcacagca cgcgtgcgac ttcgacgtcg
      781 taatcctcga tgaacgcgaa ccggccgcgg atctcggggt cctggctgaa gtggacgagc
      841 tcgcggatca tctccttgcc gccgtcgtcc tgggggtgcg ccttgccggc gaatacgagc
      901 tgcacggggc ggtcgccgga gagcagcagc gctcgcagcc tctcgcggtc ggagaggatc
      961 agcgtcgccc tcttgtactg cgcgaaccgc cgggcgaacc cgatcgtgag gatgccgggg
     1021 tcgaagacct cgtcggtcca cccgacctcg gcctccgacg cgccgcgggc gagcagctga
     1081 cggcgcaggc gctcgcgcac gaagtagacg agacgctccc gggccctctc gcgcgcacgc
     1141 cacagctccg cgtccggtac ctcctccatc cgctgccact tcgcgccgcc ggtctcggtc
     1201 cagccgggag cgagcctgcg gtcgatcacc tcggagatct cggggccgag ccacgtagcg
     1261 gtgtgcacgc cgttcgtgat cgaggtgatc gggccctctt ccgaggggat gcccggccac
     1321 aggtccgaga agatcgcgcg cgacacgacg ccgtggagct tgctgaccgc gttggcgcgg
     1381 gccgcgaggc gcaggcccat cacggccatg ttgaacgtcc cgttctcgct cgacttggtg
     1441 ccgcccgcgg agctcccctg cccgagcgcc atcagctcct cgaacgtgat gtcgcactcc
     1501 ttcgcgaacg aggcgaaata gcgctgcatg aggtcgtact cgtagacgtc gatgccggcc
     1561 ggaacgggcg tgtgcgtcgt gaagatcgtc ccggctcgga cgccttcgag cgcgtcgtcg
     1621 aagctcatgc cctcggcggt cacgatctgg cggatgcgct cgagcccgag gaatcccgcg
     1681 tggccctcgt tcgaatggaa gacgtcgggc tcgtaacccg cgacctggag cgcgcgcact
     1741 ccgccgatgc cgagcaggat ctcctggcgc agccggtgct cgctgccgcc ggcgtagagg
     1801 cggtcggtga cccccctctc ctccgcgtcg ttctcctcga cgtcggagtc catcagcaag
     1861 agcttcacgc ggccgacgcg cgccacccac agctgcgcga cgcagcgggc cccggcgagg
     1921 tcgacctcga tcttcaacgg agacccgtcg ggaccgggca ggagcgtgag cggcatcgcg
     1981 tgcggatcga gcgacgggta gcgctcctgc tgccagccgt cggcgttcag gtactggcgg
     2041 aagtagccct ggcggtagag gagcccgacg ccgaccagcg gcacgccgag gtcgctcgcg
     2101 gccttgaggt ggtcgccggc gaggacgccg aggccgccgg agtagatcgg cagcgcctcg
     2161 ctcactccga actccggcga gaagtacgcg atcgtgcgcg gcccgttctt cttgcggctc
     2221 tggtaccagc ggtcctcttc gaggtagctc tgcaggtcct cggacacgcc ggcgaggaac
     2281 gacaggaacg ggccgtcctc ggcgagctgc tcgaaccgtt cctgcgagac gaggccgagc
     2341 atgcgcacgg ggtcgtgacc ggacgcatcc cacgcctcgg agtcgaccca gcgccagagg
     2401 tcccgggcac ggcggttcca cgaccagcgg aggttcatgg cgagctcggt gagggggccg
     2461 agcttgtcgg gcagcgacac ccgcactgcg aacgagcgaa gggctctcat gggtcaagag
     2521 tctcgcgcac cggcgcgcag cgggggccga ccacgttctg tacggaatga tgtacagtga
     2581 gcacatggcc aagatagtgc ccttcaccga ggctcggtcg aacctcaccg agctcctcga
     2641 cgagctcgag gctcggcacg agcacgtgct gatcacccgg aacggccggc cgtccgccgt
     2701 ccttctctcc gcagacgagt tccaggccct cgaagaaacc ctcgacatcc tgcaggacga
     2761 cgacctcatg cgcgccctgg cacgcagcga gcgggacgta gaggaaggag acctcgtgcc
     2821 gctcgaagag cttaggcgtc cacgcgcgta gtggcccgcg ttctactgac gagcgaagcc
     2881 aggcgccact tcgacctact tcccgccacg ttccaggacg ccgtcctcga ggcgctgacg
     2941 gcgatcgagg tggacccgga gggggccggg aagcccttgc tgggtcggct gaaggggctc
     3001 tggtcgtccc gggtcggcaa ctaccgcatc ctctacacga tcgaaggcgg gcgccgctcg
     3061 ccgacggtga tcgtgcgtgc catccggcac cgcgcggtgg cttacgaagg caagaggcgc
     3121 cccagatgac gctacgtgcg gtgggggcgc agcgtcggga tctctaggta cgacgccggg
     3181 tcgtccagct cgaagaagcc cgtcagcagg gacgctcccc ggaagttcgt caccagggct
     3241 atctccgaga acgtcgtgcc gtgcagcgac gcttccggct cgattactac ctcgactacg
     3301 tgcagtgggg cggccggcgt cactaggaac tccgccgtct gcttgccgag gacgacgctc
     3361 ttgccggccc ccgccggcgt ccccaccagc tcgaggtcga cgacgacctg accgacgcct
     3421 gcaggcgtcg ctacgaacac gttcggctcg tacgtcccca ccgcgatccg gcccgtcacc
     3481 gggcgacgag catcgacgcg gagcgggagg agctccttcg ccgggaagac gttgcgccat
     3541 ccacccccgg cggccccggc ggcctcgttc gggagcccgt tcgtcaggtt ccccgcgcgg
     3601 ttcgccgcgt ccgggtagtc gcgcgcgtcc atgtagaagt cgaactcgcc ggcgacgccg
     3661 gaagcggtct cgccgatgtg gacgtagagc ttggcggtgc ggggcgcggg ggcggcgtcg
     3721 accggggccg cc
//
LOCUS       DASQDO010000313         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1011854, whole genome shotgun sequence.
ACCESSION   DASQDO010000313 DASQDO010000000
VERSION     DASQDO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011854"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VEV43_08890"
     CDS             <1..316
                     /locus_tag="VEV43_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP23676.1"
                     /translation="GHLSREFRRAYGEPPYSYLMTRRIERAMALLRRGDLSVTEVCFA
                     VGCSSLGTFSTRFTELVGMPPSTYRREAAQGTAGIPACVEKQVTRPVRNREARAPEAQ
                     LA"
     gene            322..729
                     /locus_tag="VEV43_08895"
     CDS             322..729
                     /locus_tag="VEV43_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP23677.1"
                     /translation="MDIDIHYTFLPHDDPDASLAFYRDVLGFEVRNDVGDGTMRWITV
                     GPPGGATSIVLHPPAVDPGVTDDERRTIAEMMAKGTYASIVLATKDLGGAFERVEASG
                     AEIVQEPADQPYGVRDCAVRDPAGNLVRIMELA"
     gene            726..1178
                     /locus_tag="VEV43_08900"
     CDS             726..1178
                     /locus_tag="VEV43_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYP23678.1"
                     /translation="MSAAPRPGRRRRSDTERRLTEDVDLWVATASSGGVPHLVPLSFD
                     WDGEALLMATPADSPTGRNLAATRTARLGLGHTRDVSLIEGDVEVFELDGLPGDRAER
                     FAERTGFDPRAERPLYRWFRVSPRRIQAWREANELPGRELMRDGRWLV"
     gene            1190..>2399
                     /locus_tag="VEV43_08905"
     CDS             1190..>2399
                     /locus_tag="VEV43_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYP23679.1"
                     /translation="MTATKARAKRAAPHAADTHDFIRVHGARENNLKDVSIAIPKRRL
                     TVFTGVSGSGKSSLVFDTIAAESQRLINETYSAFVQGFMPALARPDVDVLEGLTTAII
                     VDQERMGSNPRSTVGTATDANAMLRILFSRLGKPHIGSPNAFSFNVPSVRASGAITIE
                     RGPGKTKTQKATFNRLGGMCPRCEGRGAVTDFDLTALYDETKSLDEGALTIPGYSMEG
                     WYGRIFRGSGFFDTGKPIRKYTKKELHDLLYKEPTKIKVDGINLTYQGLIPQIQKSFL
                     SKDVDALQPHVRAFVERAVTFTTCPECDGTRLTKEARSSKIKGINIADACALQITDLA
                     EWVRGLKEPSVAPLLAALGHTLDSFAEIGLGYLSLDRPAGTLSGGEAQRTKMIGHLGS
                     SLTDVTYVFDE"
BASE COUNT          396 a          857 c          818 g          328 t
ORIGIN      
        1 cggtcacctc agccgcgagt tccggcgcgc ctacggcgag ccgccctact cgtacctgat
       61 gacgcgccgg atcgagaggg ccatggcgtt gctccgccgg ggcgacctca gtgtcaccga
      121 ggtctgcttc gccgtcgggt gctcgtcgct cggcacgttc agtacgcgct tcaccgagct
      181 cgtcgggatg ccgcccagca cgtaccggcg cgaggcggcg caggggacgg cggggatccc
      241 cgcgtgcgtc gagaagcagg tgacgagacc ggtcaggaat cgagaagcgc gggcccccga
      301 agctcagcta gcgtgagcgg catggacatc gacatccact acacgttcct cccgcacgac
      361 gaccccgacg cctcgcttgc cttctaccgg gacgtcctgg gcttcgaggt ccgcaacgac
      421 gtcggcgacg gcacgatgcg ctggatcacc gtcggcccgc ccggcggcgc cacgtccatc
      481 gtcctgcatc cgcccgcagt cgaccccggg gtcaccgacg acgaacggcg cacgatcgcc
      541 gagatgatgg cgaagggcac ctacgcctcg atcgtgctcg cgacgaagga cctcggcggc
      601 gccttcgagc gtgtcgaggc gagcggcgcc gagatcgtcc aggagccggc cgaccagcct
      661 tacggcgtcc gcgactgcgc cgtccgcgac cccgccggga acctcgtccg catcatggag
      721 ctggcctgag cgccgcacct cggcccggac gccggcgtcg cagcgacacc gagcgccggc
      781 tgacggaaga cgtcgacctc tgggtcgcga ccgcgtcgtc cggcggcgtg cctcatctcg
      841 tgccgctgtc gttcgactgg gacggcgagg ctctgctcat ggccacgcct gcggacagcc
      901 cgaccggcag gaacctcgca gctacgcgca cggcgcggct ggggctcggc cacacgcgtg
      961 acgtctcgct gatcgagggt gacgtcgagg tcttcgagct cgacgggctg ccgggggacc
     1021 gagccgagcg gttcgccgaa cgcaccgggt tcgatccgcg agccgagagg ccgctctacc
     1081 gctggttccg ggtctcgccc cgtcgtatcc aggcgtggcg cgaggcgaac gagctgcccg
     1141 gccgcgagct catgcgcgac ggccgctggc tggtgtgaag ggagacgcga tgacggcaac
     1201 caaggcccgg gcgaagcgcg ccgcaccgca cgccgccgac acgcacgact tcatccgggt
     1261 gcacggcgcg cgcgagaaca acctcaagga cgtgagcatc gccatcccga agcggcggct
     1321 gacggtgttc accggcgtct ccggctcggg caagagctcg ctcgtgttcg acacgatcgc
     1381 agcggagtcg cagcggctga tcaacgagac ctacagcgcg ttcgtgcagg ggttcatgcc
     1441 ggcgctggcc cggcccgacg tcgacgtcct cgaagggctg acgacggcga tcatcgtcga
     1501 ccaggagcgc atgggctcga acccgcgctc gaccgtcggg accgcgacgg acgcgaacgc
     1561 gatgcttcgc atcctcttca gcaggctcgg gaagccgcac atcggctcgc ccaacgcgtt
     1621 ctcgttcaac gtcccgtcgg tccgcgccag cggcgcgatc acgatcgagc gcggccccgg
     1681 caagacgaag acccagaagg cgacgttcaa ccgcctggga ggcatgtgcc cgcgctgcga
     1741 gggccggggc gccgtcaccg acttcgacct gaccgcgctg tacgacgaaa cgaagtcgct
     1801 cgacgagggc gcgctgacga tccccggcta cagcatggag ggctggtacg gccgcatctt
     1861 ccgcggctcc gggttcttcg ataccggcaa gccgatccgc aagtacacga agaaggagct
     1921 gcacgacctc ctctacaagg agccgacgaa gatcaaggtc gacgggatca acctcacgta
     1981 ccaggggctg atcccgcaga tccagaagtc gttcctgtcg aaggacgtcg acgcgctgca
     2041 gccgcacgtc cgcgcgttcg tggagcgagc cgtgacgttc acgacctgcc cagagtgcga
     2101 cggaacgcgg ctcaccaagg aggcgcgctc gtcgaagatc aaagggatca acatcgccga
     2161 cgcgtgcgcg ctgcagatca ccgacctcgc cgagtgggtc cggggcctga aggagccgtc
     2221 cgtcgcgccg ctgctcgccg cgctgggcca caccctcgac tcgttcgccg agatcggcct
     2281 gggctacctc tcgctcgacc ggccggcggg cacgctgtcg ggcggcgagg cgcagcgcac
     2341 gaagatgatc ggccacctcg gatcctcgct caccgacgtc acctacgtct tcgacgagc
//
LOCUS       DASQDO010000314         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1074022, whole genome shotgun sequence.
ACCESSION   DASQDO010000314 DASQDO010000000
VERSION     DASQDO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074022"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..629
                     /locus_tag="VEV43_08910"
     CDS             114..629
                     /locus_tag="VEV43_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYP23680.1"
                     /translation="MEARNGSTRTAETPATEGQADDARGTDERDEQILRLAADFENFK
                     RQAARRETEARERAVRGVIEDLLPVLDNFERAIAHGEGGEGVELVFKELKATLESAGL
                     EEVSGEGSPFDPQVHEGVDSREDESVTEEVVRAVHRRGYTMNGKLLRAAMVGVSRPAA
                     PEPVEEEVTEE"
     gene            636..1556
                     /locus_tag="VEV43_08915"
     CDS             636..1556
                     /locus_tag="VEV43_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYP23681.1"
                     /translation="MAAQRDWLEKDYYKTLGVSSTATKDEIKKAYRKLAQQYHPDTNK
                     GDAAAEARFKEISEAHAVLGNDEKRKEYDELRRYGGGGFGFRPGSPGRGGRNVRVDIG
                     DIGDLFGGNGVDLGDLFGGFGFRGRQKGADTEAEAVLSFEQAVEGATIELSGAKVRIP
                     PAVTDGTRIRVAGKGTPGPHGGEAGDLYVVVRVRPHPVFAQGRDGDLVVEVPVTFPEA
                     ALGANVTVPTLDGHVTVKVPAGTQHGRTLRVRGKGGPRPRGGTGDLLVKIGIEVPRKL
                     SRKEREALERFAEVHEASPRAGLEHETTTT"
     gene            1569..2021
                     /locus_tag="VEV43_08920"
     CDS             1569..2021
                     /locus_tag="VEV43_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYP23682.1"
                     /translation="MSRRPDDDNQAIYIISVAAELAGVHPQTLRVYERKGLLSPSRTS
                     GNTRRYSRKDIELLRRIQDLTNEGVNLAGVMRILELEGEIERLRTRVARAGAQLEEKQ
                     RQIEQLRAARTTVALVPLKDVRRVRRAMKADEVDQAGRRRTLEAPPIS"
     gene            2023..2652
                     /locus_tag="VEV43_08925"
     CDS             2023..2652
                     /locus_tag="VEV43_08925"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP23683.1"
                     /translation="MAELLGDGPEDGRAVIVLAHGAGAGMRSDFMAFQAAALAEAGLL
                     CLRFEFPYMQAGRKAPDKAAVLEESWRAVAEDVRARTGERPLVLGGKSMGGRIASQAV
                     AAGAPADGLVFHGYPLHPPGRPDTGRTAHLPDVGAPMLFVEGTRDPFCPLDTLRDVIA
                     ETGIDATVHVVADGDHSFKVRASSGRSTKDAWAEVAERTVAWIDETLLP"
     gene            2653..>3206
                     /locus_tag="VEV43_08930"
     CDS             2653..>3206
                     /locus_tag="VEV43_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23684.1"
                     /translation="MRRPAASVYLTIEAVTGFAFYTMATLASVYRISSAGLDPLQLVL
                     VGTALEATVFLFEIPTGILADTVSRRRSVIVGTFLTGIGFMIEASFPRFLPILLGQVV
                     WGLGYTFVSGANVAWVTDEVGEAPAAGLYLRGAQLANFAALGGIVASVSLGSVALWMP
                     IFAGGIVFVLLSLFLMVAMPESGFT"
BASE COUNT          507 a         1101 c         1166 g          432 t
ORIGIN      
        1 cgggtggaaa tgcttccaat gcgtcggggc aggattcctc ggcgcgacat ggcggaacgc
       61 agggccattc acatggagcg cgggccaggg tgcgcagtgc tgaggcgcag ggcgtggaag
      121 cgcgcaatgg aagtacacga accgctgaga cgccggcaac agaaggacaa gccgacgacg
      181 ctcgcggcac cgacgagcga gacgagcaga ttttgcggtt agccgccgac tttgagaact
      241 tcaagcgcca ggcggcgcgg cgcgagaccg aggcccgcga gcgcgccgtc aggggggtca
      301 tcgaagattt gctgccggtg ctcgacaact tcgagcgcgc gatcgcgcac ggtgagggcg
      361 gcgagggcgt cgagctcgtg ttcaaggagc tcaaggcgac gctcgagagc gccggcctcg
      421 aggaggtctc cggcgagggc tctccgttcg acccccaggt ccacgaaggc gtcgactcgc
      481 gcgaggacga gtcggtgacc gaagaggtcg tgcgtgcagt gcatcgccgc ggctacacga
      541 tgaacggcaa gctgctgcgc gcggcgatgg tcggagtgtc gcgcccggcc gcgcccgagc
      601 ccgtcgaaga agaagtgacc gaggagtagg agacgatggc agcccagcgc gactggctcg
      661 agaaggacta ctacaagacg ctgggcgtct cctcgaccgc gaccaaggac gagatcaaga
      721 aggcgtaccg aaagctcgcc cagcagtacc accccgacac gaacaagggc gacgcggcgg
      781 ccgaggctcg cttcaaggag atctcggagg cgcacgcggt cctcggcaac gacgagaaac
      841 gcaaggagta cgacgagctg cgccgctacg gaggcggcgg gttcggcttc cgtccgggct
      901 cgccgggacg aggcggccgc aacgtgcgcg tggacatcgg ggacatcggc gatctcttcg
      961 gcggcaacgg cgtcgacctc ggcgacctct tcggagggtt cggcttccgc gggcgccaga
     1021 agggcgcgga caccgaagcc gaggccgtcc tgtcgttcga gcaggcggtc gagggagcga
     1081 cgatcgagct ctccggggcg aaggtccgga tcccgccggc cgtcaccgac ggcacgcgca
     1141 tccgcgtcgc gggcaaggga acgcccggcc cccacggggg cgaggccggc gatctctacg
     1201 tcgtcgtgcg cgtgcgcccg catccggtgt tcgcgcaggg ccgcgacggc gacctcgtcg
     1261 tcgaggtgcc ggtgacgttc cctgaggccg cgctcggcgc gaacgtcacc gtgccgacgc
     1321 tcgacggcca cgtcactgtg aaggtgccgg cgggcaccca gcacggccgc acgctgcgcg
     1381 tgcgcgggaa aggggggccg cggccccgcg gaggaaccgg cgacctcctc gtgaagatcg
     1441 gcatcgaggt cccgcgcaag ctgtcgcgca aagagcgcga ggcgctcgag cgcttcgcgg
     1501 aggtacacga ggcgtcgccg cgggcgggcc tcgaacacga gacgaccact acctaggggg
     1561 gaggcaccgt gtcccggcga ccggacgacg acaaccaggc gatctacata atctcggtcg
     1621 cggccgagct tgcgggcgtc cacccgcaga cactgcgcgt ctacgagcgc aagggcctgc
     1681 tctcaccgag ccgcacgagc ggcaacaccc gccgctactc gcgcaaggac atcgagctcc
     1741 tgcgccgcat ccaggacctg acgaacgagg gcgtcaacct cgcgggagtc atgcggatcc
     1801 tcgagctcga gggcgagatc gaacgtctcc gcacccgcgt cgcgcgggcc ggcgcgcagc
     1861 tcgaggagaa gcagcgccag atcgagcagc tccgggccgc gcgcacgacg gtcgcgctcg
     1921 tgccgttgaa ggacgtgcga cgggtccggc gcgcgatgaa ggccgacgag gtcgaccagg
     1981 ccggccgccg ccgcacgctc gaagccccgc cgatctcctg acgtggccga gctcctcggc
     2041 gacggtcccg aagacggccg cgcggtaatc gtcctcgcgc acggcgcggg ggccgggatg
     2101 cgcagcgact tcatggcgtt ccaggccgcc gccctcgcgg aagcgggcct cctgtgcctg
     2161 cgcttcgagt tcccgtacat gcaggccggg cgcaaggccc ccgacaaggc cgccgtgctc
     2221 gaagaatcgt ggcgcgccgt tgctgaggat gtgcgcgccc gcaccggcga gcggccgctc
     2281 gtgctcggtg gcaagtcgat gggcggccgc atcgcgtcgc aggctgtggc cgcgggtgca
     2341 ccggccgacg gcctcgtgtt ccacggctac ccgctccacc cgccgggccg gcccgacacg
     2401 ggccgcacgg cccacctccc cgacgtcggc gcgccgatgc tgttcgtgga agggacgcgc
     2461 gacccgttct gcccgctgga caccttgcgc gacgtgatcg ccgagacggg gatcgacgcg
     2521 accgtccacg tggtcgccga cggcgaccac tcgttcaagg tgcgtgcgtc gtcgggccgt
     2581 tcgacgaagg acgcgtgggc cgaggtggcc gaacgcaccg tcgcctggat cgacgagacc
     2641 ctgctcccgt aagtgcgccg tccggccgcg tcggtctatc tcacgatcga agccgtcacg
     2701 ggcttcgcct tctacacgat ggcgacgctc gcgtccgtgt accggatctc gtcggcaggc
     2761 ctcgacccgc tccagctcgt gctcgtcggc accgcgctcg aggcgaccgt gttcctgttc
     2821 gagatcccga ccgggatcct cgccgacacg gtcagccggc ggcgctccgt catcgtcggg
     2881 acgttcctca cgggcatcgg cttcatgatc gaggcgtcgt tcccgcgctt tctgccgatc
     2941 ctgctgggcc aggtcgtgtg gggcctcggc tacacgttcg tcagcggcgc caacgtcgcg
     3001 tgggtcaccg acgaggtcgg cgaggcaccg gcggccggcc tgtacctgcg cggcgcacag
     3061 ctggcgaact tcgccgcgct cggcggcatc gtcgcgagcg tctcgctcgg cagcgtcgcg
     3121 ctgtggatgc cgatcttcgc cggagggatc gtgttcgtgc tgctgtcgct cttcctgatg
     3181 gtcgcgatgc ccgagagcgg cttcac
//
LOCUS       DASQDO010000315         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1196509, whole genome shotgun sequence.
ACCESSION   DASQDO010000315 DASQDO010000000
VERSION     DASQDO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196509"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VEV43_08935"
     CDS             complement(<1..520)
                     /locus_tag="VEV43_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23685.1"
                     /translation="MSLQRRLTLFFVLIVILPLVAAGYVVQRVVVGEISRRAVVALDP
                     ALDAAVAVYNARVGDMVDEVRRTVERPRVARLMADRDADAVKKFLVGALERSGELDFL
                     ILLDEEGRVLGHARTPGEFVDGYERPDPGDVLAAEGASGPGFNRTRLIPLRIGGEGRV
                     GSVVGGFWLDRSI"
     gene            593..895
                     /locus_tag="VEV43_08940"
     CDS             593..895
                     /locus_tag="VEV43_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HYP23686.1"
                     /translation="MPIYEYACTSCGERTEAKQGFDDPPLEDCPHCGGKLRKLFSPVG
                     IVFKGSGFYSTDAKTGSKSSGSSDKSDSAKTESKSETKADKPATPKKDTSATEKSA"
     gene            892..1686
                     /locus_tag="VEV43_08945"
     CDS             892..1686
                     /locus_tag="VEV43_08945"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HYP23687.1"
                     /translation="MTAAAVGVFGGSGFYSLLDDVRHEEVATPYGDPSAPIAIGRVGD
                     IDVAFLPRHGRKHQLPPHRINYRANVWALKELGVTRIVGPCAAGSLQRHVEPGHFVVC
                     DQLVDRTTGRTDTFYDGPVTTHVSFADPYCPELRPLAIEAARSEGITVHERGTVVTIQ
                     GPRFSTRAESRWFQDAGWHVVNMTQYPEAYLARELEICYVNVSLITDYDVGVAGEDDP
                     VSHEAVLEVVQANNEKLRRLLFALVPRIPAERSCPCSTALRGARIE"
BASE COUNT          314 a          663 c          637 g          263 t
ORIGIN      
        1 ggatcgaccg gtcgagccag aagccgccga ccacggagcc gacgcggccc tcgccgccga
       61 tgcgcagcgg gatcagccgc gtccggttga agccggggcc ggacgctccc tcggcggcca
      121 gcacgtcgcc ggggtcggga cgctcgtatc cgtcgacgaa ctcaccgggg gtgcgggcgt
      181 gcccgaggac gcgcccctcc tcgtcgagca ggatcaggaa gtcgagctcg cccgacctct
      241 cgagcgcgcc gacgaggaac ttcttcacgg cgtctgcgtc gcggtccgcc atgagccgcg
      301 ccacccgggg ccgctcgacc gtgcggcgga cctcgtcgac catgtcgccc acccgcgcgt
      361 tgtacacggc gacggccgcg tcgagcgcag gatccagggc cacgacggca cggcgggaga
      421 tctcgcccac gacgacgcgc tgcactacgt acccggccgc gacgagcggc aggatgacga
      481 tgaggacgaa gaagagcgtt aggcgccgct ggagggacat cgggtggtca ctaccccctt
      541 tggagggttc gtcggcgctc tgagacctcg attttagtgc gatgatcccg ccatgcccat
      601 ctatgaatac gcctgcacga gctgcggtga gcgcaccgag gcgaaacagg gcttcgacga
      661 cccgcccctg gaggattgcc cgcactgcgg cggcaagctc aggaagctgt tctcgcccgt
      721 cgggatcgtg ttcaaagggt cgggcttcta ctcgaccgac gcgaagaccg gctcgaagtc
      781 gagcgggtcg agcgacaagt ccgattcggc gaagaccgag tcgaagtccg agaccaaggc
      841 cgacaaaccg gccacgccga agaaggacac ctcggccacg gagaagtcgg cctgacggca
      901 gccgccgtcg gagtcttcgg cggatccggc ttctacagcc tcctggacga cgtccgccac
      961 gaggaggtcg ccacaccgta cggagacccg tccgcgccga tcgcaatcgg acgcgtcggc
     1021 gacatcgacg tcgcgttcct gccgcgccac ggccgcaagc accagctccc gccgcaccgg
     1081 atcaactacc gcgcgaacgt ctgggccctc aaggagctcg gtgtcacccg gatcgtcggg
     1141 ccgtgcgccg cggggtcgct gcaacgccat gtcgagcccg gccacttcgt cgtctgcgac
     1201 cagctcgtcg acaggaccac cggccgtacc gacaccttct acgacggccc cgtcacgacg
     1261 cacgtctcgt tcgccgaccc gtactgcccg gagctgaggc cgctcgcgat cgaagctgcg
     1321 cgctcggagg gcatcacggt ccacgagcgc ggcaccgtcg tgacgatcca ggggccgcgc
     1381 ttctcgacga gggccgagtc ccgctggttc caggacgccg ggtggcacgt cgtgaacatg
     1441 acgcagtacc cggaggcgta tctcgcgcgg gagctcgaga tctgctacgt caacgtctcg
     1501 ctcatcaccg actacgacgt cggcgtcgcc ggcgaggacg accccgtgag ccacgaggcc
     1561 gtcctggagg tcgtgcaggc gaacaacgag aagctgcggc ggctcttgtt cgcgctggtg
     1621 ccgaggatcc cggccgagcg gagctgcccg tgcagcaccg cgctccgggg cgcgcggatc
     1681 gagtagacga gaacggcccc ccgtgtccgg agggccgttc tttacttcga cgatcgatca
     1741 gtcgtagcag ccgagcttct cgggcttgag gtcttccgcg atcatgcacg gctcctcgag
     1801 gatgcagaag ttcacgggcc cgtccaccga gcacgcgtgt gccggtgccg gcgcgactgc
     1861 gagcagcgcg cccgctc
//
LOCUS       DASQDO010000316         5941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_584825, whole genome shotgun sequence.
ACCESSION   DASQDO010000316 DASQDO010000000
VERSION     DASQDO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584825"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VEV43_08950"
     CDS             complement(<1..628)
                     /locus_tag="VEV43_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase"
                     /protein_id="HYP23688.1"
                     /translation="MPQHHVSHPRGSDLGPRSVMLEGRFGRMFRNLPSFLPPDEALKA
                     VADTMVEPAQAAPTDGWAPSGGDRDSGIPAGYTYLGQFVDHDITFDPVSSLQRQNDPD
                     ALHDFRTPRFDLDSLYGGGPNNSPYMYDPDVDGGRTTLLLGRNEVGEEDLPRNVLGPD
                     SLGEPRGRALIGDPRNDENVIVSQLHLTFVKFHNAVVERVRAEQPGLEH"
     gene            748..1605
                     /locus_tag="VEV43_08955"
     CDS             748..1605
                     /locus_tag="VEV43_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP23689.1"
                     /translation="MEHALPPGQPVTVGASTVNVVALGEGPSILLLHGWPETSRRWRD
                     VAGALARHHHVVAMDLPGFGDSGPSPGGYTTPALAATALDVMSQLGVATFDVAGHDWG
                     GSVAYALAATARDRVTRLVVEEELLPGFRVDPDGAALGTYPVWHGDFHRIPDLPEVLV
                     RGHEDAYYGYFWDLTAEPGAIGRAARDEYLRTYASHGALSAGLALYRAAEEDARHNRA
                     LAAAPLTIPVLAVGGSRAIGHGVARSLACVAGDVREHVVHACGHYPAEEHPGEWLRVV
                     EGFLSGDVP"
     gene            1747..1956
                     /locus_tag="VEV43_08960"
     CDS             1747..1956
                     /locus_tag="VEV43_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23690.1"
                     /translation="MLIGGASTALAPAASADIPTACEILGNVVEAVEDDCKATTGFVG
                     DTIDRQADNVFFVFCTAFPDHYACQ"
     gene            2097..2342
                     /locus_tag="VEV43_08965"
     CDS             2097..2342
                     /locus_tag="VEV43_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23691.1"
                     /translation="MHENLIELGYSEKKGMIEVTLPSGFTSQKFGRISDRLIADLINR
                     LPRGCQACLSGESLIIRERLENVLRVDLDSMEIVEKG"
     gene            2352..3266
                     /locus_tag="VEV43_08970"
     CDS             2352..3266
                     /locus_tag="VEV43_08970"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HYP23692.1"
                     /translation="MPLTDTSRAGTLRELPKLGVGLLYNPSLPEYLRNHLASYDYLSI
                     IPDMFWTDAGAAASPRFVELPSWMEMLEWVRARKPVIAHNIGMSLGSAGPFDTDYVAH
                     LREWHRRYDFEWHSDHLSFVRVTGAEGHDHNAGLAVPVPYDREVLEMIAARIESVQES
                     IEAPFLIENNVYFVDVPDQDMTEPQFLNELSARTGCGILLDLHNVYANARNHGFDARS
                     FVGELDLDRVGEIHIAGGSEIGDVYTDSHAGPCPEPVWELLEWTVPQAPNLAGITFEF
                     HDSYYPLLKAEGIDAEMTRARAVWGRSH"
     gene            3269..3868
                     /locus_tag="VEV43_08975"
     CDS             3269..3868
                     /locus_tag="VEV43_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23693.1"
                     /translation="MSLLGYQQALCDLIASPASCLQLRGGDTAVLDGYDLDDRDRRRL
                     RTVVSQRGMSTACTLYRVNRITPIFTLLPLTCFLLGDDLIGEAEAFWQETVATDLQFS
                     PEASRFAAFVRQRIEEGAVDNPYVEEVMELELALNRVNFAGRAERGETRVRFRHDPEA
                     LLGPLSRRQLPPEPPAEGEFWVVVDASGPEVELRAEGAG"
     gene            3938..5281
                     /locus_tag="VEV43_08980"
     CDS             3938..5281
                     /locus_tag="VEV43_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23694.1"
                     /translation="MTTSWRAGPLVRFVAIATVALVAATACGETSKVDPSAKVSISGR
                     VTTPSGEPLADRPVRLATGASGGDALLGVITLGLACTAGICDDTVRSAVTRDDGTYRF
                     VLRGRDTQTSFGNVRTQVLSASAAPAGDQVSGASMAAQFVVQTERVRLPPLELVDPAL
                     SVEAASGRAVASWEPSPGAPYTLAFETDSVVPVWQVTTAETGATVDSRVLEGSSGRVV
                     VSGGFDDTIEGSDIHLTWRSPGVGYAAAGAPLSRDAACRYGNARSASLPGTGGDCGLT
                     DGDLFFAETPVARCPGQPSPRRKRACPQPTTATIDMEGAAPVDLVVVRGCSSRCPVEV
                     SANGRRWRAAGAATSPFATVRLPEATVRSVRVGLGVDGLREVSVWGPEAQLEPGLQPV
                     PEAELEVLREPYEAVEPEESGGVPGIVWVGAGALLIALVAMAYRAGRRRGAASQP"
     gene            complement(5311..5382)
                     /locus_tag="VEV43_08985"
     tRNA            complement(5311..5382)
                     /locus_tag="VEV43_08985"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5348..5350),aa:Val,seq:cac)
     gene            5578..5784
                     /locus_tag="VEV43_08990"
     CDS             5578..5784
                     /locus_tag="VEV43_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23695.1"
                     /translation="MRKLVLTVMAASAIVTSGGVASAEEITIPPIGREGCEVSGGTVG
                     VLEPDGAVTYDNFYVDPYFCLPPP"
BASE COUNT          882 a         2065 c         2099 g          895 t
ORIGIN      
        1 cgtgctcgag ccccggctgc tcggcccgca cccgctcgac cacggcgttg tggaacttca
       61 cgaacgtcag gtggagctgc gacacgatga cgttctcgtc gttgcgaggg tcgccgatca
      121 gcgcgcgccc gcggggctcc ccgagcgagt ccggccccag gacgttccgc gggaggtctt
      181 cctccccgac ctcgttgcgc ccgaggagca acgtggtccg cccaccgtcg acgtcggggt
      241 cgtacatgta cggcgagttg ttcggtccgc ccccgtacag cgagtcgaga tcgaaccggg
      301 gcgtcctgaa gtcgtgcagc gcgtccgggt cgttctgccg ctggaggctc gacacggggt
      361 cgaacgtgat gtcgtggtcg acgaactggc cgaggtaggt gtagccggcg gggatgccgc
      421 tgtcgcggtc gccgccggag ggggcccatc cgtccgtagg cgcggcctgc gccggctcga
      481 ccatggtgtc cgcgaccgcc ttcagggcct cgtccggcgg gaggaacgac ggaaggttcc
      541 ggaacatccg gccgaagcgg ccttccagca tcaccgagcg agggccgagg tccgatcccc
      601 gagggtgact gacgtggtgc tgaggcatgc cgcctcccct ctgcaccgga aaggtgcatc
      661 cgcctgatcg tcaactccga cactatctag gagtcatctc tcgtgacaag aggcaggggg
      721 cgcccgatcg gttgcatcat ccaaggcgtg gaacacgcct tgccccccgg gcagccggtg
      781 acggtgggag cgtccaccgt caacgtcgtc gccctcggcg aagggccctc gatcctgttg
      841 ctacacggct ggcccgagac gtcgaggagg tggcgcgacg tcgcgggcgc gctcgcacgc
      901 caccatcacg tcgtcgcgat ggacctgccg gggttcggag attccggccc gagccccggg
      961 ggatacacca cccccgcact cgcggcgacc gctctggacg tgatgtcgca gctcggcgtc
     1021 gcgacgttcg acgtcgccgg gcacgactgg gggggttcgg tcgcatacgc gctcgcggcg
     1081 acggcgcgcg accgtgtgac gcgcctcgtc gtcgaagagg agctgctgcc cgggttccga
     1141 gtcgacccgg acggagccgc gctcggcacc taccccgtgt ggcacggcga cttccaccgc
     1201 atccccgacc tcccggaggt cctcgtccgc ggtcacgagg acgcgtacta cgggtacttc
     1261 tgggacctga cggccgagcc cggtgcgatc gggcgcgcgg cgcgcgacga gtacctgagg
     1321 acctacgcgt cccacggggc actcagcgcg gggctggcgc tgtaccgggc ggccgaggag
     1381 gacgcccgcc acaaccgcgc ccttgccgcg gcccccctca ccatccccgt cctcgccgtc
     1441 ggcgggagcc gggcgatcgg tcacggcgtg gcgcgctctc tggcatgcgt cgcaggagac
     1501 gtccgcgagc acgtggtgca cgcttgcggc cactatcccg cggaggaaca ccccggcgaa
     1561 tggctgcgcg tggtggaggg cttcctctcc ggagacgtcc cgtagcgtcg ctgcgcggac
     1621 gatttccgtc gccccggcag gagagcacta gtcgtccccc gaaaggtgcc ccagcggctt
     1681 cagcggcgaa gccctggact ggggaggtag aggatgcgtc gagcttcagc gatcctgtct
     1741 ttcgtcatgc tcatcggggg ggcgagcacg gccctggcgc cggcggcttc ggccgacatc
     1801 ccgaccgctt gcgagatcct cggcaacgtc gtcgaggcgg tcgaggacga ctgcaaggcg
     1861 acgacggggt tcgtcggcga cacgatcgac cgtcaggccg acaacgtgtt cttcgtcttc
     1921 tgcacggcgt tccccgacca ctacgcctgc cagtaacacc accgagcaac gaacgaagag
     1981 gggccttcgg gcccctcttt ctcgtagcgg cgcgggcatc gcgccccttc ttgacacgtc
     2041 aacctgacgg gtgtcacggt ggggggcaag atcgaaccgg acaggggagg gcgacgatgc
     2101 acgagaacct gatcgagctg gggtactccg agaagaaggg catgatcgag gtcacgctgc
     2161 cgagcggctt cacctcacag aagttcggac gcatcagcga ccgcctcatc gccgacctca
     2221 tcaacaggct gccgcgtggc tgccaggcgt gcctctcggg tgagtccctg atcatcagag
     2281 agcgcctcga gaacgtcctc cgcgtcgacc tcgactcgat ggagatcgtc gaaaagggct
     2341 gatccgggcc ggtgccgctg accgacacct cacgcgccgg gacgctccgg gagctgccga
     2401 agctgggagt cgggttgctc tacaacccgt ctcttccgga gtacctgcgc aaccatctcg
     2461 cgtcgtacga ctacctgtcg atcatccccg acatgttctg gacggacgcg ggcgccgcgg
     2521 cgtcgccgcg gttcgtcgag ctcccgtcgt ggatggagat gctcgagtgg gtccgcgctc
     2581 gcaagccggt gatcgcgcac aacatcggca tgtcgctcgg gtccgcgggc ccgttcgaca
     2641 ccgactacgt cgcgcacctc cgtgagtggc accggaggta cgacttcgaa tggcacagcg
     2701 accacctgtc gttcgtccgc gtgacgggcg ccgagggcca cgaccacaac gcggggctcg
     2761 cggtgccggt cccctacgac cgcgaggtac tcgagatgat cgcggctcgc atcgagagcg
     2821 tgcaggagtc gatcgaggct ccgttcctaa tcgagaacaa cgtctacttc gtggacgtcc
     2881 ccgaccagga catgaccgag ccgcagttcc tcaacgagct cagcgcgcgc accggatgcg
     2941 ggatcctcct cgacctgcac aacgtctacg cgaacgcccg caaccacggc ttcgacgcgc
     3001 ggtcgttcgt cggggagctc gacctcgacc gggtcggcga gatccacatc gccggcggca
     3061 gcgagatcgg cgacgtctac acggattcgc acgcgggccc gtgcccggag ccggtctggg
     3121 agctactcga gtggacggtc ccgcaggccc ccaacctggc ggggatcacg ttcgagttcc
     3181 acgactccta ctacccgctc ctcaaagccg agggcatcga cgccgagatg acgagagcgc
     3241 gggccgtctg gggtcgctcc cactgaccgt gtcgctcctc ggctaccagc aggccctgtg
     3301 cgatctgatc gcgtcgccgg cgtcgtgcct ccagctgcgc ggcggcgaca ccgcggtcct
     3361 cgacggatac gacctcgacg accgtgaccg gagacgtctg cggaccgtgg tgagtcaacg
     3421 cgggatgtcg acggcctgca ccctctaccg cgtcaaccgg ataacgccga tcttcacgct
     3481 gctcccgctg acgtgcttcc tcctcggtga cgacctgatc ggcgaagccg aggcgttctg
     3541 gcaggagacg gttgcgaccg acctccagtt ctcgcccgag gcctccaggt tcgcggcgtt
     3601 cgtcagacag cgcatagagg aaggggcggt cgacaacccg tacgtggagg aggtgatgga
     3661 gctcgagctc gcgctgaacc gggtcaactt cgccggccgg gcggaacgcg gggagacgag
     3721 ggtgcgtttc cgtcacgatc ccgaagcgct gctgggtccc ctgtcgcgcc ggcagctccc
     3781 gccggagccg ccggcggagg gagagttctg ggtcgtggtc gacgcatcgg gcccggaggt
     3841 cgagctgcgc gccgaggggg ccggctgacg gccgcatgtc ggtggtccgg ccgagcggcc
     3901 cctgtgcaag gcgccggacc tgcgccagga tgagtgtgtg acgacgtcgt ggcgcgccgg
     3961 ccccctcgtg cgcttcgtcg cgatcgcgac cgttgcgctc gtcgcggcca ccgcctgcgg
     4021 cgagaccagc aaggtcgacc cgagcgccaa ggtgtcgatc tcgggacggg tcacgacccc
     4081 gtccggggag ccgctcgccg atcgccccgt ccgcctggcg accggtgcat ccggcgggga
     4141 cgcgctcttg ggcgtgatca ccctgggact ggcctgcacc gccggcatct gcgacgacac
     4201 cgtccggtcg gccgtcacgc gggacgacgg cacgtatcgc ttcgtgctgc gcggccggga
     4261 cacccagacg tcgttcggca acgtcaggac gcaggtcctc tcggcatccg ccgctcccgc
     4321 tggagaccag gtgtccgggg catcgatggc ggcgcagttc gtcgtccaga cggagcgggt
     4381 ccggctcccg ccgttggagc tcgtggatcc cgcgctgtcg gtggaagccg cctccggccg
     4441 cgcggtcgcg tcgtgggaac cgtcaccggg cgcgccctac acgttggcat tcgagacgga
     4501 ctcggtcgtc ccggtgtggc aggtcaccac ggcagagacc ggggccaccg tcgacagccg
     4561 cgtcctcgaa ggctccagcg gacgcgtcgt cgtgtcgggc gggttcgacg acacgatcga
     4621 gggcagcgac atccacctca cctggcggtc gccgggcgtc ggctacgcag cggcgggcgc
     4681 gccgctgtcg agagacgctg cgtgccgcta cggcaacgcg agatcggcct ctcttcccgg
     4741 caccgggggc gattgcggcc tcaccgacgg agacctcttc ttcgcggaga cgccggtggc
     4801 acgctgcccg gggcagccgt ctccgcgccg gaagcgggcc tgcccccagc cgacgaccgc
     4861 gacgatcgac atggaggggg ccgcgcccgt cgacctcgta gtcgttcgtg gttgttcgag
     4921 cagatgtccc gtggaggtat ccgcgaacgg gcggagatgg agggccgcgg gtgcggccac
     4981 gtcccccttc gcgacggtgc ggctgccgga agccaccgtg cgttccgtcc gcgtcggtct
     5041 gggcgtcgac gggcttcgag aggtctcggt ctgggggccg gaggcgcaat tggagccggg
     5101 gctacagccg gtcccggaag cggagctgga agtcctgcgc gaaccctacg aagctgtcga
     5161 gcccgaggag tcagggggcg tcccggggat cgtctgggtg ggggccggtg cgctgctgat
     5221 tgcgctcgtg gcgatggcgt accgagcagg ccgccggcgc ggtgccgcat cgcaaccctg
     5281 aacgtggccc tgatgccgcg gaacacgggg tgggcggtac tgggatcgaa ccagtgacct
     5341 ctgccgtgtg aaggcagcgc tctcccgctg agctaaccgc ccgggagcac gcattctagc
     5401 ggcggtcgcg gaccccttcg cggcacaggt cggacgttcc ggcgcccgtc gcgggagcgc
     5461 gggccggccc ggcgaaccga cgatccagcg acccaaccag gcgggcgcaa tccgggcgct
     5521 cggcagcggc ggccggcttt ctcttacacg ctgccgccga aggaggtgaa acagaccatg
     5581 cgcaagctcg tgctcaccgt catggccgcc tctgcgatcg tgacgtcggg cggggtggcc
     5641 tccgccgagg agatcaccat cccccccatc ggccgcgaag gctgcgaggt cagcggaggg
     5701 acggtcggcg tgctggagcc ggacggcgcc gtcacgtacg acaacttcta cgtggacccg
     5761 tacttctgcc tgccgcctcc gtaagcaagc acccgggccc gcgcgggcca cgcgcccgcg
     5821 cgcggccccc gggccgcgac ccggttcagg cccggccggg gcaggtctcc actacctcct
     5881 cggcaaggtc gaagaagctg atccccttct cgaagtacat gtcggcgccg agagagagcg
     5941 c
//
LOCUS       DASQDO010000317         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_217390, whole genome shotgun sequence.
ACCESSION   DASQDO010000317 DASQDO010000000
VERSION     DASQDO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217390"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VEV43_08995"
     CDS             <1..591
                     /locus_tag="VEV43_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYP23696.1"
                     /translation="TFRGRAAHAGVEPEKGRSAVLEAAHKVVALHALNGRWPSVTVNA
                     GVVRGGTRPNVVAERCDLHVDVRASTVQEFEAARAEVERIVTTTTVDGVRAEMSPTAG
                     HRPMEKTPASEALVSLAIRIAGEMGFDLHDAATGGASDANTTSAEGLPTLDGLGPVGG
                     DDHSVDEWLDIESVVPRVTLLAALMDRAAEALPASG"
     gene            604..1068
                     /locus_tag="VEV43_09000"
     CDS             604..1068
                     /locus_tag="VEV43_09000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYP23697.1"
                     /translation="MSADAFRHRTALDVRFSDIDAFGHVNNAVFFSYAEQARIRYLLD
                     VLQPDAPFDRLPLILARIELDFRSPIFFGDALTVETRVHRIGRTSFEMSHRMTAGTEG
                     RLVGDVGSVLVTYDYDRSEPIPVPAPWRQAMAAHEGHSLDSDGPRRAVAAAS"
     gene            1082..>1836
                     /locus_tag="VEV43_09005"
     CDS             1082..>1836
                     /locus_tag="VEV43_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP23698.1"
                     /translation="MAEPQSFDVVILGGGMSYVGAIRAAQLGLSVGMVERDRLGGTCL
                     NRGCIPSKALLETADLLHRVTEQGAEFGLSGHDGIGLEYGALAKRRDAVIEKHVGGVE
                     FLMKKNRVTVIAGNGVVTGPRTVRVSGGPSGDVEMTGKNLVLATGSAPKSLPGLEIDG
                     ERIITSDEALARNDVPGRVTIVGAGAIGVEWASMYRDFGAEVTLVEFADRVVPLEDPE
                     ISKELARVFRKRGIGIHVSSTIDPESLDRTSDA"
BASE COUNT          274 a          625 c          670 g          267 t
ORIGIN      
        1 acgttccgcg gccgtgccgc gcacgcgggc gtcgagcccg agaaggggcg gagcgcggtg
       61 ctcgaggcgg cgcacaaggt cgttgcgctg cacgccctca acggccgctg gcccagcgtg
      121 acggtgaacg ccggtgtcgt ccggggcggg acgcgtccga acgtcgtcgc cgagcgctgc
      181 gacctccacg tcgacgtccg ggcgtccacg gtccaggagt tcgaggccgc gcgcgcggag
      241 gtcgagcgca tcgtgacgac caccacggtc gacggcgtcc gcgcggagat gtcacccacg
      301 gcggggcacc gtccgatgga gaagacgccc gcgtccgagg ctctcgtgtc gctggcgatc
      361 cggatcgcgg gtgagatggg tttcgacctg cacgatgccg ccaccggagg cgcgagcgac
      421 gcgaacacca cgtctgcgga gggcctcccg acgctcgacg gcctgggacc cgtcggcgga
      481 gacgaccatt cggtcgacga gtggctcgac atcgagagcg tcgttccccg cgtcacactg
      541 ctcgcggctc tcatggatcg cgccgcggag gcgctcccgg cgtccggcta gaatggcgct
      601 cccgtgagtg ccgacgcctt tcgccatcgc accgcgctcg atgtccgttt cagcgacatc
      661 gacgccttcg ggcacgtgaa caacgccgtc ttcttcagct atgcggagca ggcgcggatc
      721 cgctacctgc tcgacgtcct ccagcccgat gcgcccttcg accgtctgcc gctgatcctc
      781 gcccgcatcg agctcgactt ccgctcgccg atcttcttcg gtgacgcgtt gacggtcgag
      841 acgcgcgtcc accgcatcgg ccggacgagc ttcgagatga gccaccggat gacggccggc
      901 accgagggac gtctcgtcgg tgacgtcggc agcgtcctcg tgacgtacga ctacgatcgc
      961 tcggagccga tcccggttcc ggccccctgg cggcaggcga tggcagcgca cgagggccac
     1021 tcgctggaca gcgatggtcc gcgccgcgcg gtcgccgcgg cgagctgagc agcgtcgcca
     1081 catggccgag ccccagtcgt tcgacgtggt catcctcggc ggcggcatga gctacgtcgg
     1141 cgcgatccgc gcggcgcagc tggggctctc cgtcggaatg gtcgagcgtg accggctggg
     1201 cggcacgtgc ctcaaccgcg gctgcatccc gtcgaaggcc ctcctggaga ccgccgatct
     1261 gctgcaccgc gtgaccgagc agggcgcgga gttcggcctc agcggccacg acgggatcgg
     1321 gctcgagtac ggggcgctcg cgaagcgacg tgacgccgtc atcgagaagc acgtcggggg
     1381 cgtcgagttc ctcatgaaga agaatcgggt gacggtgatc gccggcaacg gcgtggtgac
     1441 gggcccgcgg acggtgcggg tgtcgggcgg gccatcgggc gacgtcgaga tgaccggcaa
     1501 gaacctcgtg ctcgccacgg ggtccgcgcc gaagtcactg ccggggctcg agatcgacgg
     1561 agagcggatc atcacctcgg acgaggcgct ggcgaggaac gacgtccccg ggcgggtgac
     1621 gatcgtgggt gccggtgcga tcggcgtgga gtgggccagc atgtaccggg acttcggtgc
     1681 cgaggtgacc ctcgtcgagt tcgccgaccg ggtggtgccg ctcgaggatc ccgagatcag
     1741 caaggagctc gcgagggtct tcaggaagcg cggcatcggg atccacgtct cgagcaccat
     1801 cgaccccgaa tccctggacc gcacgtcgga cgcgga
//
LOCUS       DASQDO010000318         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1074651, whole genome shotgun sequence.
ACCESSION   DASQDO010000318 DASQDO010000000
VERSION     DASQDO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074651"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /gene="aroA"
                     /locus_tag="VEV43_09010"
     CDS             <1..1145
                     /gene="aroA"
                     /locus_tag="VEV43_09010"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYP23699.1"
                     /translation="TAAAVAALGARVDLEDEDNVEVEGCGIGGLREPDGVLDLGNSGT
                     SMRTLLGVCAGIEGLSVLTGDASLRSRPMLRVAAPLRQMGAAVDGRAYGELPPMTVRG
                     GNLRGIEAELNVASAQVKTAILLAGLAATGTTSVTEPGPSRDHTERMLAAAGVEVARD
                     GTTCSVEGGSQPGPRAWEVPGDTSSALYLIVAALLVSGSELTVTDVLLNPTRTAALDV
                     LIRMGADLTVEETTESGGEPRGDVTARTSDLTATDVEPREVPGLIDELPLLAIAASQA
                     EGKTTVSGAAELRVKESDRIAAMTAGLRALGAEAEERPDGMVIEGPARLTGGAVDSFG
                     DHRIAMSFAIAGLLSAEGVRVSRWSCVDTSFPGFLDVLGRAQGRVA"
     gene            1147..>1635
                     /gene="cmk"
                     /locus_tag="VEV43_09015"
     CDS             1147..>1635
                     /gene="cmk"
                     /locus_tag="VEV43_09015"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYP23700.1"
                     /translation="MAALAIDGPAGAGKSTVARAVARALGWRYVDSGAMYRALALAAL
                     RAGVDPADEDAVGSLAETAEIDLDGTRVWLEGTDVSERIRASDVTKIVSTVAANPRVR
                     SALLGKQRALAETSDVVMEGRDIGSAVLPRAEFKVYLTAAPAERARRRALEQGLPQDD
                     STL"
BASE COUNT          217 a          579 c          628 g          211 t
ORIGIN      
        1 cgaccgccgc cgccgtcgcc gcactgggcg cccgggtcga cctcgaggac gaggataacg
       61 ttgaagtaga gggttgcgga atcggcgggc tgagggaacc ggacggcgtt ctcgacctcg
      121 gcaactccgg gacctcgatg cggacccttc tcggggtctg cgcggggatc gaaggcctga
      181 gcgttctcac cggagacgcc tcgttgcggt cccggccgat gctccgggtc gcggcccccc
      241 tgcgccagat gggcgccgcc gtcgacggcc gggcctacgg cgagcttccc ccgatgaccg
      301 ttcgcggcgg gaacctcagg gggatcgaag ccgagctcaa cgtggcgagc gcgcaggtga
      361 agaccgcgat cctcctcgcc ggcctggcgg ccacggggac gacgagcgtc accgagcccg
      421 gccccagccg cgaccacacc gaacggatgc tcgcggccgc cggcgtggag gtcgcccggg
      481 acgggacgac ctgctcggtc gaaggcgggt cgcagcccgg gccgcgcgcc tgggaagtgc
      541 cgggcgacac gtcctctgcc ctgtacctga tcgtcgcggc cctcttggtg tccgggtccg
      601 agctcacggt gacggacgtg ctgctcaacc cgacgcggac cgcggccctc gacgtcctca
      661 tccggatggg tgccgacctg accgtggagg agacgaccga gtccggcggc gagccccgcg
      721 gcgacgtcac ggcgcgcacg tcggacctga ccgcgaccga cgtcgagcct cgcgaggtcc
      781 cggggctgat cgacgagctc ccgctcctcg ccatagcggc gtcgcaggcc gagggcaaga
      841 cgacggtgtc cggagccgcc gagctgcgcg tcaaggagtc ggaccggatc gccgcgatga
      901 ctgcggggct gcgcgcgctc ggggccgagg ccgaggagcg gcccgacggg atggtgatcg
      961 agggcccggc ccggctcacc ggcggcgcgg tcgactcgtt cggcgaccac cgcatcgcga
     1021 tgtcgttcgc gatcgccggc ctgctgtcgg ccgagggcgt gagggtctcg cgctggtcgt
     1081 gcgtcgacac ctcgttcccc gggttcctcg acgtgctcgg ccgggcgcag ggacgggtgg
     1141 cctgacgtgg ctgccctggc gatcgacgga cctgctggcg cgggcaagag cacggtggcg
     1201 cgagcggtgg cgcgcgctct cggctggcgc tacgtcgact ccggcgcgat gtaccgcgca
     1261 ctcgcgctag cggccctgcg ggcgggcgtc gaccccgccg acgaggacgc ggtggggtcg
     1321 ctggccgaga ccgccgagat cgacctcgac gggacccgtg tgtggctcga ggggaccgac
     1381 gtctccgaac ggatccgggc ttccgacgtc acgaagatcg tctcgacggt cgcggcgaac
     1441 ccgcgggtgc gcagcgcgct cctcggcaag cagcgcgcgc tggcggagac gtccgacgtc
     1501 gtgatggaag gccgcgacat agggagcgcg gtcctcccgc gcgcggagtt caaggtctac
     1561 ctgacggccg ctccggccga gcgggcgcgc cggcgcgcgc tcgagcaggg ccttccgcaa
     1621 gacgactcga cgctc
//
LOCUS       DASQDO010000319         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_33798, whole genome shotgun sequence.
ACCESSION   DASQDO010000319 DASQDO010000000
VERSION     DASQDO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33798"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VEV43_09020"
     CDS             <1..349
                     /locus_tag="VEV43_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYP23701.1"
                     /translation="PAAVGAYSQAIVANGFVYCSGQVPLDPQSGELIGGSIADQTRRC
                     LENLSAVLRAAGAGLDDVVKVTAFLVDMNDFPEFNEAYGEFFGDEPPARATVAVAGLP
                     KGAEVEVECVATV"
     gene            complement(375..593)
                     /locus_tag="VEV43_09025"
     CDS             complement(375..593)
                     /locus_tag="VEV43_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23702.1"
                     /translation="MNSYQVFLAGVLVTWPLIIFGLLFLMSRMEDYVNRSDAATPEEA
                     GLEPVEGHSGEREVQIRFGDQVVGESSK"
     gene            623..1786
                     /locus_tag="VEV43_09030"
     CDS             623..1786
                     /locus_tag="VEV43_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HYP23703.1"
                     /translation="MNADFATFADRDRLVDRLQQLVRTPSENPPGEEAAAAALTAAFC
                     EELGLDTELHEVEPGRPSVVARWRGSGGPALCYCSHIDVVPAGDPSLWERDPYSGAIE
                     DGRMHGRGSSDAKGPVAAALEAVAILKRAGWEPQGTLELALVADEETMGFKGAGYLVE
                     EKILNPDVAMVGEPTSLRVVRAQRGASWFRLTTRGVAGHGSAPERGVNAIKHMAEVVL
                     HLEDTLPDVTHPLLGGPTVSVGTIRGGEKVNIIPAACVAEVDRRTVPGETRESVIAGI
                     QEAVERARKRFPDVDVYVDLAFFAESFEVAPDAPVVAAAVDALRTTTGRDVDVIGFRG
                     ASDARFLFETGADVIVCGPGDITLAHTAHESIDLAELEHGAVAYAHAFATLLS"
     gene            1783..2799
                     /gene="trpD"
                     /locus_tag="VEV43_09035"
     CDS             1783..2799
                     /gene="trpD"
                     /locus_tag="VEV43_09035"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYP23704.1"
                     /translation="MSFSWPQVLGRLLGGDSLSEDEAAACMEEIMEGAASPAQVAGFV
                     VALRAKGETIDEVAGLVQTMRRYAEKVEVEGDVLDTCGTGGDRSGTFNVSTAAAIVCA
                     GAGAKVAKHGNRAASSRCGSADVLEALGVKIDLPPAGVEACIAEAGIGFCFAPLFHPA
                     MRHAGPTRRDLGVPTIFNFLGPLTNPAGATRQALGVSDPKMIEKMVHTLGRLGSKHVV
                     AFHGDKGLDELSTSGPSDVYELVEGEVRRWRIDPEELGLPVSPLETVAGGTADENAAT
                     IRDVLEGEAGPRRDIVVLNAAAGLVAAGLAPDVNEGLRQAGEAVDSGEAARRLALLVE
                     TSSA"
     gene            complement(2801..3979)
                     /locus_tag="VEV43_09040"
     CDS             complement(2801..3979)
                     /locus_tag="VEV43_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HYP23705.1"
                     /translation="MSVRRALAGVVVLTQLAVPLAGSGSAQTPPCTWSIGLMGALSGD
                     AARYGRPIADGIRLAVDLANRERDLPCALAVHAKDSQGDPTQAPALARALVDDEQVVA
                     CVCGFFSGETLATGPIFDDGGLLMASTGSNRTIDEQGYDTWFRAVAADDRQGAAAARY
                     IVEVLRPRSVSVVHDNQAYSKSLAEDVADGVGRRLEGKFVINPEEADYSAVVARLEKI
                     RPDVVFYGGYSPQAGLLLRQMREAGVRSRFVTDEGAKNGWMRRYLRRSDGRARLTAVC
                     PCSDPTEIAAASTFVDEYVASYGKRPRGYAADGFDMTNIAIDALRGLSGTETVEQARA
                     HVVAHFDAAEDVEGTVKDYTWDESGELVTNRTHVFMWRWLDRRERFEYVGRVSELTQP
                     "
BASE COUNT          587 a         1463 c         1454 g          613 t
ORIGIN      
        1 ccccgccgcc gtcggcgctt attcacaggc gatcgtcgct aacggcttcg tctactgctc
       61 cggccaggtc cccctcgacc cccagagcgg cgagctgatc ggcggctcga tcgcagacca
      121 gacgcgccgg tgtctcgaga acctctcggc ggtactgcgc gcggcggggg cgggtctcga
      181 cgacgtcgtc aaggtcacgg cgttcctcgt cgacatgaac gacttcccgg agttcaacga
      241 ggcctacggc gagttcttcg gcgacgagcc ccccgcacgc gcgacggtcg ccgtcgcggg
      301 gctcccgaag ggcgccgagg tcgaggtcga gtgcgtcgcc accgtctgac ggcgcgtacg
      361 atcccgcgat gcagctactt cgaggactcc ccgacgacct ggtcgccgaa cctgatctgg
      421 acctcgcgct cgcccgagtg accctcgacg ggctcgagcc ccgcctcctc cggcgtcgcg
      481 gcgtcggagc ggttgacgta gtcctccatg cggctcatca ggaaaagcag gccgaagatg
      541 atcaacggcc acgtcacgag gacgcccgcg aggaagacct ggtagctgtt catcgcccac
      601 agtttggcag gtatcgatgc cggtgaacgc ggatttcgcg accttcgcag accgcgatcg
      661 gctcgtcgac cggttgcagc agctcgtccg cacgccgtcg gagaacccgc ccggagaaga
      721 ggcggccgcg gctgcgctga ccgccgcgtt ctgcgaggag ctcgggctcg acaccgagct
      781 gcacgaggtc gagccggggc ggccgagcgt cgtcgcgcgc tggcgcggtt ccggcggccc
      841 ggccctctgc tactgctcgc acatcgacgt cgtcccggcg ggcgacccct cgctgtggga
      901 gcgcgaccct tactcgggcg cgatcgagga cggacgcatg cacggacgcg ggtcgtcgga
      961 tgcgaagggc cccgtcgcgg ccgcgctcga agccgtggcg atcctgaagc gcgcgggatg
     1021 ggagccgcag ggcacgctcg agctcgcgct cgtcgccgac gaggagacga tgggcttcaa
     1081 gggcgccggg tacctcgtcg aggagaaaat cctgaacccc gacgtggcga tggtggggga
     1141 gcccacgtcg ttgcgggtcg tgcgcgctca gcggggcgcg agctggttcc gcctgacgac
     1201 gcgcggcgtc gccggtcacg ggtcggcccc cgagcgcggc gtcaacgcga tcaagcacat
     1261 ggccgaggtc gtcctgcacc tcgaggacac gctgccggac gtgacgcacc cgctgctcgg
     1321 cggcccgacc gtcagcgtcg gcacgatccg cgggggcgag aaggtcaaca tcatcccggc
     1381 ggcgtgcgtc gccgaggtcg accggcgcac ggttcccggc gagacgaggg agtccgtgat
     1441 agcggggata caagaggccg tcgagcgtgc gcgcaagcgt ttcccggacg tcgacgtcta
     1501 cgtggacctc gcgttcttcg ccgagtcctt cgaggtagcg cccgacgcgc ccgtcgtggc
     1561 cgcagcggtc gacgcgcttc gcaccaccac cggacgcgac gtcgacgtca tcgggttccg
     1621 cggcgcgtcc gacgcgcgct ttctcttcga gaccggcgcc gacgtcatcg tgtgcggtcc
     1681 cggcgacatc acgctcgcgc acaccgcgca cgagtcgatc gacctcgccg agctcgagca
     1741 cggcgcggtt gcctacgcgc acgcgttcgc gacgctgctg tcgtgagctt ctcctggccg
     1801 caggtcctcg ggcggctcct cggcggcgac tcgctctcgg aggacgaggc cgcagcgtgc
     1861 atggaggaga tcatggaggg cgccgcgagc cccgcgcagg tcgcgggctt cgtcgtcgcc
     1921 ctgcgcgcga agggcgagac catcgacgag gtcgccggcc tcgtgcagac gatgcgccgt
     1981 tacgccgaga aggtcgaggt cgagggcgac gtcttggaca cgtgcgggac cggcggcgat
     2041 cgcagcggca cgttcaacgt ctcgacggcg gccgcgatcg tgtgtgcggg ggccggggcg
     2101 aaggtcgcca agcacgggaa ccgtgcggcc tcgtcccgct gcggctcggc cgacgtgctc
     2161 gaggccctcg gcgtcaagat cgacctcccc ccggccggcg tcgaggcctg catagcggag
     2221 gcggggatcg ggttctgctt cgcccccctc tttcacccgg cgatgcgcca cgcaggcccc
     2281 acccggcggg acctcggcgt ccccacgatc ttcaacttcc tcggccccct cacgaacccg
     2341 gcgggtgcga cccgccaggc cctcggcgtc tcggacccga agatgatcga gaagatggtc
     2401 cacacgctcg gacgcctcgg cagcaagcac gtcgtggcgt tccacgggga taagggtcta
     2461 gacgagcttt cgacttcggg cccgtccgac gtctacgagc tggtcgaggg tgaggtccgc
     2521 aggtggcgga tcgacccgga ggagctgggg ctgccggtgt cgcccctgga gaccgtggcc
     2581 ggaggcaccg cggacgagaa cgccgccacg atccgagacg tcctcgaagg cgaggccggg
     2641 ccgcgacggg acatcgtcgt gctgaacgcc gccgcggggc tcgtcgccgc gggcctggcg
     2701 ccggacgtga acgaaggtct ccggcaggcg ggtgaggccg tggattcggg cgaggccgcc
     2761 cggcggctcg cgctgctcgt cgagacctcg tccgcctgac ctacggctgg gtcagctcgg
     2821 agaccctccc gacgtattcg aaacgctcgc gccggtctag ccacctccac atgaagacgt
     2881 gggtccggtt cgtgacgagc tcaccgctct cgtcccaggt gtagtccttg accgttccct
     2941 cgacgtcttc cgccgcgtcg aagtgcgcga ccacgtgagc tcgggcttgc tcgaccgtct
     3001 cggtacccga gagcccgcgc agcgcgtcga tcgcgatgtt cgtcatgtcg aacccgtcgg
     3061 cggcgtagcc cctcggccgt ttcccgtaac tcgccacgta ctcgtcgacg aacgtggacg
     3121 cggctgcgat ctcggtgggg tcggagcacg ggcagacggc ggtcaatcgc gcgcgcccgt
     3181 cggatcgccg gaggtacctg cgcatccagc cgttcttcgc tccctcgtcg gtgacgaacc
     3241 gggaccggac cccggcttct ctcatctgcc gcagcagcag gccggcctgg ggtgagtagc
     3301 ccccgtagaa gacgacgtcg ggcctgatct tctcgagccg ggctaccacc gcgctgtagt
     3361 ccgcctcttc cggattgatg acgaacttcc cttcgagacg gcgcccgact ccgtccgcca
     3421 cgtcctctgc aagcgacttc gaataggcct ggttgtcgtg gacgacgctc accgaccggg
     3481 gtctcaggac ctccacgatg tagcgcgcgg cggccgcacc ctgtctgtcg tcggccgcga
     3541 ccgcgcggaa ccacgtgtcg tagccctgct cgtcgatcgt gcggttgctc ccggtcgacg
     3601 ccatcagcag tccgccgtcg tcgaagatcg gtccggtagc cagggtctct ccggagaaga
     3661 agccacagac gcacgcaacg acctgctcgt cgtcgacgag cgcgcgggcg agggcggggg
     3721 cctgggtcgg gtcaccttgc gagtccttgg cgtgcacggc gagggcgcag gggaggtccc
     3781 gttcccggtt ggcgaggtcg acggccaagc ggatgccgtc cgcgatgggc cggccgtagc
     3841 gagcggcgtc gccgctgagc gcgcccatca acccgatcga ccacgtgcag ggcggggtct
     3901 gtgcgctccc ggaccctgcc agggggacgg cgagctgggt cagcaccacc acccccgcga
     3961 gcgcccggcg gaccgacatt gccccagcgt tacacaccgg ggcgccgagc gtcagatccc
     4021 ggccccctcg gcgcgggact cgctcggctg gacgtcgtcg agccgccgtc cctcgcgcag
     4081 cctcgacgcc ccgaacggcg acaggtcgat cgtctcg
//
LOCUS       DASQDO010000320         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_156922, whole genome shotgun sequence.
ACCESSION   DASQDO010000320 DASQDO010000000
VERSION     DASQDO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156922"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /gene="tig"
                     /locus_tag="VEV43_09045"
     CDS             <1..1259
                     /gene="tig"
                     /locus_tag="VEV43_09045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYP23706.1"
                     /translation="PALDAVYRRWAGEVKVSGFRKGKVPRQIIDARVGPEVIREEALR
                     DALPDLYRDALEAEGLEAIAPPEIEVLEFDAGSPILFEAVVDVRPEVRIPDLAAISID
                     APPSEVTDEEVSEQLDRLREGFASLEPVGREARRGDFVLIDLNGYRHDEPVEGASAPD
                     DLYEVGSRTGPPRLDEELEGARPGAILKFNDAIPSGELAGEEISFTVLVKEVKVKQLP
                     PLDDELAKTMGEFDTLAELEDDIRERLAPVKRGMVREEIANQALTALVDASDLVAPDK
                     LVEGELEHRLEHFEEDVKRAGLTMDEYTQRANLTDLEIRRDIRRQVERSVKAELLLEE
                     IARNERFDVTEEDIGREVALLAHRTGRDAKEVAEQVVNAGRLGSLAADIMRRKALEYV
                     VEAINVTGRPAEEPMPQEEEAEAHPS"
     gene            1256..>1779
                     /locus_tag="VEV43_09050"
     CDS             1256..>1779
                     /locus_tag="VEV43_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYP23707.1"
                     /translation="MTQQPHNYLVPMVVEQTSRGERAFDIYSRLLKERIIFLGTPVDD
                     QVANLIVAQMLHLESEDPDKDISLYINSPGGQVYAGMAIYDTMQYIKPDVSTICVGMA
                     MSMGAILLCGGANGKRFSLPNAKIMIHQGSAGYQGTAADIEIHAREILDVNQRMIEII
                     SEHTGQTTEQVRKDL"
BASE COUNT          337 a          613 c          593 g          236 t
ORIGIN      
        1 agcccgcgct ggacgcggtg tacagaaggt gggccggaga ggtgaaggtc tccggcttcc
       61 gaaagggcaa ggtcccgcgc cagatcatcg acgcccgcgt gggccccgag gtcatccgcg
      121 aggaggctct gcgggacgcc ctgcccgacc tctaccggga cgcgttggag gccgagggcc
      181 tcgaggccat cgccccgccg gagatcgagg tgctcgagtt cgacgccggc tcgccgatcc
      241 tgttcgaggc cgtcgtcgac gtgcggcccg aggtccggat ccccgacctc gccgcgatct
      301 cgatcgacgc gccgccgtcg gaggtcaccg acgaggaggt ctccgagcag ctggaccggc
      361 tccgggaggg gttcgcctcg ctcgagcccg tcggccgcga ggcccgccgc ggcgacttcg
      421 tcctcatcga cctgaacggc taccgccacg acgagcccgt cgaaggggcg agcgcccccg
      481 acgacctcta cgaggtcggc tctcgcaccg ggcccccccg actcgacgaa gagctcgagg
      541 gcgcccggcc cggcgccatc ctgaagttca acgacgcgat cccctccggc gagctggccg
      601 gcgaggagat ttccttcacc gtcttggtga aggaggtgaa ggtgaagcag ctcccgccgc
      661 tcgacgacga gctcgcgaag acgatgggcg agttcgacac gctcgccgag ctcgaggacg
      721 acatccgcga gcggctcgcc ccggtcaagc ggggcatggt ccgtgaggag atcgcgaacc
      781 aggcgctgac ggccctcgtc gacgcctcgg acctcgtcgc ccccgacaag ctggtcgaag
      841 gcgagctcga gcaccggctc gagcacttcg aggaggacgt gaagcgggcc ggcctcacga
      901 tggacgagta cacccagcgc gcgaacctga ccgacctcga gatccgccgc gacatccgcc
      961 ggcaggtgga acggtcggtc aaggccgagc tcctcctcga ggagatcgcc cgcaacgagc
     1021 gcttcgacgt caccgaggag gacatcggac gcgaggtcgc gctgctggcg caccgcacgg
     1081 gccgggacgc gaaagaggta gcggaacaag tcgtgaacgc agggcgtttg ggctctctgg
     1141 ccgccgatat catgcgtcga aaggccctcg aatacgtcgt agaggccata aacgtgaccg
     1201 gccggccggc cgaagaaccc atgccacaag aagaagaagc ggaggcgcac ccctcatgac
     1261 ccagcaaccg cacaactacc tcgtcccgat ggtcgtcgag cagacgagcc ggggcgagcg
     1321 tgcgttcgac atctactcgc ggctcctcaa ggagcggatc atcttcctcg ggacccccgt
     1381 cgacgaccag gtcgcaaacc tgatcgtggc gcagatgctc cacctcgagt ccgaggaccc
     1441 cgacaaggac atcagcctct acatcaactc gccggggggc caggtctacg ccggcatggc
     1501 gatctacgac acgatgcagt acatcaagcc cgacgtctcg accatctgcg tgggcatggc
     1561 gatgtcgatg ggagcaatcc ttttgtgcgg cggagccaac ggcaagcggt tctcgctccc
     1621 caacgcgaag atcatgatcc accagggctc ggcgggctat cagggcaccg cggccgacat
     1681 cgagatccac gcccgcgaga tcctggacgt caaccagcgc atgatcgaga tcatctcgga
     1741 gcacaccggg cagacgaccg agcaggtgcg caaggacct
//
LOCUS       DASQDO010000321         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1136033, whole genome shotgun sequence.
ACCESSION   DASQDO010000321 DASQDO010000000
VERSION     DASQDO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136033"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VEV43_09055"
     CDS             <1..457
                     /locus_tag="VEV43_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYP23708.1"
                     /translation="NKADWLTGMAAATGVLGIAFGRWWADAVAAAVISADILHDGVSN
                     VRNAVADLMDTSPSKVDGSGRDPLPARVANEVAKLPWVERARVRFREEGHVYYGEVFV
                     VPAGGQVDAARLDEATEMVRRLDWRIYDVVVVAVGGLNEHEGEGTDAAG"
     gene            468..1049
                     /locus_tag="VEV43_09060"
     CDS             468..1049
                     /locus_tag="VEV43_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23709.1"
                     /translation="MDRRLRASPLWLGLLALGGLLAGHLVSYFVVAPDAHERAELLAH
                     TGHAHGGLLGTLALAATFAAVIGLFMQRVRSSGERGSRAGVAALLWVVQTTGFVLLET
                     WERGHGIPGVAELLHEPAFLIGLVAQLAVALAATALVLLIRATADALRRLLAPRARSS
                     AAITFSFETVVRARDSVARAAWNLRGPPSRACP"
     gene            1079..1774
                     /locus_tag="VEV43_09065"
     CDS             1079..1774
                     /locus_tag="VEV43_09065"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HYP23710.1"
                     /translation="MRRVVLGALAGALVAAFAAPAAAHVTVQPSEAISGTFSRFVVRV
                     PNERPDADTTKVVVKMPPLTFVSFEPRDGWKRKVKMVELEEPIEAFGQEITEAVGKVT
                     WKGGRIGPGEFLEFGFSARMPDGEETLTFEALQTYSGGEVVEWTGAPDSETPAASLTT
                     YDLGLEEGQGQLAALAELRGGGTSEADDAADDDDEEEGTGTATVLSGAALLFAVIALV
                     LAMRRGGTGATTA"
BASE COUNT          245 a          648 c          668 g          258 t
ORIGIN      
        1 gaacaaggcc gactggctga cggggatggc cgcggcgacg ggggtcctcg ggatcgcgtt
       61 cgggcgctgg tgggccgacg cggtcgcggc ggccgttatc tccgcggaca tcctccacga
      121 cggggtctcg aacgtccgca acgcggtcgc ggacctcatg gacacctcgc cgtcgaaggt
      181 cgacggctcc gggcgcgacc cgctgccggc ccgcgtggcg aacgaagtcg cgaagctccc
      241 gtgggtcgaa cgggcgcggg tgcgcttccg cgaggaaggc cacgtctact acggcgaggt
      301 cttcgtcgtc ccggccggcg gacaggtcga cgcggcccga ctcgacgagg cgaccgagat
      361 ggtgaggcgg ctcgactggc ggatctacga cgtcgtggtc gttgccgtcg gtggcctgaa
      421 cgagcacgag ggggagggaa cggacgccgc cggatagcat cggctccgtg gaccggcgac
      481 tgcgcgcgtc accgctgtgg ctggggctcc tcgccctcgg cgggctgctc gcgggccacc
      541 tcgtctccta cttcgtcgtc gcccccgacg cgcacgagag ggccgagctg ctcgcccaca
      601 ccggtcacgc ccacggcggc ctcctcggaa cgctcgcgct cgccgccacc ttcgccgccg
      661 tcatcggcct gttcatgcag cgcgtgcgct cgagcggcga gcgcggctcc cgcgccggcg
      721 tcgccgcgct cctgtgggtc gtccagacga cgggattcgt gctcctcgag acgtgggagc
      781 gcggacacgg catccccggg gtcgcagagc tcctccacga gcccgcgttc ctgatcgggt
      841 tggtcgccca gctcgcggtc gcgctcgcgg cgaccgcgct cgtcctgttg atccgcgcca
      901 cggccgacgc gctgcggcgg ctcctggcac cgcgggcccg cagctccgcg gcgatcacgt
      961 tctcgttcga gaccgtcgtc cgcgcccgcg actcggtcgc gcgcgccgcg tggaacctgc
     1021 gcggcccccc ttctcgcgcc tgtccttagc cgttctcgaa aagacaggag cgttacggat
     1081 gagaagagtt gtcttgggcg cgctcgccgg cgcgctggtc gcagcattcg ccgccccggc
     1141 cgcggcccac gtcacggtcc agccctcgga ggccatatcg gggacgttct cgcgcttcgt
     1201 ggtgcgggtc ccgaacgagc ggcccgacgc cgacaccacg aaggtcgtcg tcaagatgcc
     1261 gccgctcacg ttcgtgagct tcgagccgag ggacggatgg aagcgcaagg tcaagatggt
     1321 cgagctcgag gagccgatcg aggcgttcgg ccaggagatc accgaggcgg tcgggaaggt
     1381 cacgtggaag ggcggccgca tcggcccggg tgagttcctc gagttcggct tctcggcacg
     1441 catgcccgac ggtgaggaga cgctgacgtt cgaggcgctc cagacctaca gcggcggcga
     1501 ggtcgtcgag tggaccggcg cgcccgactc cgagaccccg gcggcttcgc tgacgacgta
     1561 cgacctcggc ctcgaagagg gccagggcca gctcgctgcg ctcgccgagc tgcgcggcgg
     1621 cggaacgtcc gaggccgacg acgccgcgga cgacgacgac gaggaggaag ggaccggcac
     1681 cgccaccgtc ctgagcggag ccgcgttgct cttcgccgtg atcgcgctcg tgctcgcgat
     1741 gcgacgaggc gggacgggag cgaccaccgc gtgaggaagc cccgatgctt cgtcgccgcc
     1801 gtcacgatcg ccgcggccc
//
LOCUS       DASQDO010000322         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_462939, whole genome shotgun sequence.
ACCESSION   DASQDO010000322 DASQDO010000000
VERSION     DASQDO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462939"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VEV43_09070"
     CDS             complement(<1..352)
                     /locus_tag="VEV43_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23711.1"
                     /translation="MDGTQPAAEPIELQLERRRGELTAYCYRMLGSSFEAEDAVQETF
                     MRAWRSFDGFEGRAELSTWLYKIATNVCLDMLAADKRRVRPMDLQPARSADTPLPDPL
                     AEETWIEPAPDSLVV"
     gene            complement(436..2202)
                     /locus_tag="VEV43_09075"
     CDS             complement(436..2202)
                     /locus_tag="VEV43_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP23712.1"
                     /translation="MIRRLRLHNWRAYEDLDLELGPGATFVVASNGIGKTSLIMGAGW
                     GLFGDAAGVHAAREIRGDADSATVAIELRLPSGADATITRTVDRKGKVALEAEVDGRT
                     ITSQDELDELLIRELGADPKVLAQLTIMIHGAAETSQGQFELRDHLAEVFGVTPLFEG
                     ARAAKALADQTAKELRKTRDVQRTERRGRDELVAELEALVEELGEANEVREGKAAAIE
                     ETSVSIRLGDEWARYRAGLADRTGRLGALAQEATAVLDATPAPDDLVETLAGREAALA
                     AAVAAAEQDAAVARAKAGLVRGAIDDLAGAGATCPTCLRPLSEHDAEEAAAEHARHLE
                     ELDAQAAAADERAAEASAALGPLRDLLERARSMPVPLEPETEIDEAEVAMARARLETH
                     QRELQEIDGKIAVLSAKGAELQGALAAADEEDERFRELEALYRREGIGRAAHDALVAT
                     GNAITERYIEPLAKEVEGRWKSMFGSGGLSLSSEGHITRKLGGRTLEFGSLSGGEKVW
                     ALLLTRLLILGASTRAPFVWLDEPLEHLDPKLRKVVAGTLAKASSGAGIRQVIVTTYE
                     AELARQLMEDVPSASLLYVTSG"
     gene            complement(2199..3080)
                     /locus_tag="VEV43_09080"
     CDS             complement(2199..3080)
                     /locus_tag="VEV43_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23713.1"
                     /translation="MADSDRVQDALAAYLDHLEMGGAEPDFSHLTEEERAELQELVDA
                     LELTEGIAFGSGGEQSPAPVAATPEGERFLEALRDLLPPRVRLEADDNRLVARIGDVA
                     IVDRFVLGTFGGRVRAWVVDAGSAGAVEDDGEAFADLGRAFRMFPDTSAIALVGRDLS
                     CLVVEPEDTAPQIRVPAGSLVARRYKRAVGPVTDALPAYLDELIPYWTPMPAFDRDAG
                     VHIDVGEVAGDLVRAAVENQRGIGGRARKGNPKKDALSAFGDREVRAVDALVEALFDG
                     SLSPAGVEARIDKAAGR"
     gene            complement(3070..3624)
                     /locus_tag="VEV43_09085"
     CDS             complement(3070..3624)
                     /locus_tag="VEV43_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23714.1"
                     /translation="MRASVLRTWRSNSEVIAGVDPWDVVDEAWAAMAEQGFATKGDFL
                     PHALTVARNKAIDHQRRVEARRRDRSIDLPEAEEVPGSIGADVDYFRSREQIGAVGRL
                     ALWEEAIHEPGLLTELQRRVFVAVRIDGKSRAAVGRELDPPLTGQRVGQITAEAFIKL
                     QRYVKRNEDRTLGAGRRGGAGHGG"
     gene            3765..>3989
                     /locus_tag="VEV43_09090"
     CDS             3765..>3989
                     /locus_tag="VEV43_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYP23715.1"
                     /translation="MAATEETLEIAGREVRITSPEKVFFPERGETKLDLVRPYLRFEE
                     PVMRTMGGRPLLLQRFPNGATGSSFFQKRIP"
BASE COUNT          528 a         1484 c         1358 g          619 t
ORIGIN      
        1 cgacgaccaa agaatccggg gccggctcga tccacgtctc ctcggcgagc ggatcaggga
       61 gcggcgtatc cgcggacctt gcgggctgga gatccatcgg gcggacgcgc cgcttgtccg
      121 ctgccagcat gtcgaggcat acgttcgtgg cgatcttgta gagccacgtg ctcagctcgg
      181 cacggccctc gaaaccgtcg aagctccgcc acgcgcgcat gaacgtctcc tgcaccgcgt
      241 cctccgcctc gaatgaggag ccgagcatgc ggtagcagta cgccgtcagc tctccgcgcc
      301 ggcgttcgag ctggagctcg atcggctctg cagccggctg tgtcccgtcg atcacttctc
      361 acctccgagc aggtcgtctt ggtgagtaat cgagggggcc ggaaaaccga aagcgcctgg
      421 agcgcagaaa gatcgtcagc cggacgtgac gtacaggagc gaggccgacg ggacgtcctc
      481 catcagctgc cgtgcgagct cggcctcgta cgtcgtgacg atgacctgcc ggatgcccgc
      541 gccggacgac gccttcgcga gcgtgccggc cacgaccttg cgcagcttcg ggtcgaggtg
      601 ctccaacggc tcgtcgagcc acacgaacgg cgcacgcgtc gacgcgccga gtatcagcag
      661 ccgggtgagc aacagcgccc acaccttctc gccgccggag agcgagccga actcgagggt
      721 ccggcccccg agcttgcgcg tgatgtgccc ctccgacgac aggctcaggc cgccgctgcc
      781 gaacatcgac ttccaccggc cctcgacctc cttggcgagc ggctcgatgt agcgctcggt
      841 gatcgcgttg ccggtggcca cgagcgcgtc gtgcgccgcg cgaccgatgc cctcccggcg
      901 gtagagagcc tcgagctcgc ggaagcgctc gtcctcttcg tctgcggccg cgagcgcgcc
      961 ctgcagctcc gcgcccttcg ccgagagcac cgcgatcttc ccgtcgatct cctggagctc
     1021 ccgctggtgc gtctccagcc gggcgcgagc catggcgacc tcggcttcgt cgatctcggt
     1081 ctcgggctcg agcgggacgg gcatcgagcg cgcgcgttcg aggaggtcgc ggagggggcc
     1141 gagcgcggcg ctggcctcgg ccgcccgctc gtccgccgcg gccgcctgcg cgtcgagctc
     1201 ctcgaggtgc ctcgcgtgct cggccgcggc ctcttcggcg tcgtgctccg agagcgggcg
     1261 cagacaggtc ggacacgttg cccccgcacc cgcgagatcg tcgatcgccc cccttacgag
     1321 cccggccttg gcgcgggcga cggcggcgtc ctgctcggcg gccgccaccg ccgcggcgag
     1381 cgcggcctcg cgccccgcga gcgtctcgac gagatcgtcg ggggccggcg tcgcgtccaa
     1441 cacggccgtc gcttcctgcg cgagggctcc caagcggccc gtccgatcgg caagccccgc
     1501 gcggtagcgc gcccattcgt cgccgagccg gatcgacacg gaggtctctt cgatcgcggc
     1561 ggctttcccc tcgcgtacct cgttcgcctc gcccagctcc tcgacgagcg cttcgagctc
     1621 ggcgacgagc tcgtcgcgcc cgcgcctctc cgtgcgctgc acgtcgcgcg tcttgcgcag
     1681 ctccttggcg gtctggtcgg cgagggcctt cgcggcccgg gccccctcga acagcggcgt
     1741 cacgccgaag acctccgcga ggtggtcccg gagctcgaac tgtccctgcg acgtctccgc
     1801 cgcgccgtgg atcatgatcg tgagctgcgc cagcaccttc gggtcggcgc cgagctctcg
     1861 aatcaacagc tcgtcgagct cgtcctgcga cgtgatcgtg cggccgtcca cctccgcttc
     1921 gagcgcgacc ttgcccttgc ggtcgaccgt gcgcgtgatc gtcgcgtccg cgccggacgg
     1981 gagccggagc tcgatcgcca ccgtcgcgga gtcggcgtcg ccgcggatct cccgcgcggc
     2041 atgcaccccg gccgcatcgc cgaagagccc ccagccggcc cccatgatca gcgacgtctt
     2101 gccgatgccg ttcgacgcga cgacgaacgt cgcgccgggg ccgagctcga ggtcgaggtc
     2161 ctcgtacgcc cgccagttgt ggagccggag ccgccggatc atcggccggc cgccttgtcg
     2221 atgcgcgcct cgacgccggc gggggacagg ctcccgtcga acagggcttc tacgagcgcg
     2281 tcgacggcgc gcacctcgcg gtcgccgaac gcgctgagcg cgtccttctt cgggttgccc
     2341 ttgcgcgccc gcccgccgat cccgcgctgg ttctcgaccg cggcgcgtac gaggtcgccg
     2401 gcgacctcgc cgacgtcgat gtgcacgcct gcgtcgcgat cgaacgcggg catgggggtc
     2461 cagtagggga tcagctcgtc gaggtatgcc ggcagcgcgt cggtgacggg gccgacggct
     2521 ctcttgtagc ggcgggcgac gagcgagccg gcggggacgc ggatctgcgg agcggtgtcc
     2581 tcgggctcga cgacgaggca cgagaggtcg cggcccacga gcgcgatcgc tgacgtgtcc
     2641 gggaacatcc tgaaggcgcg gccgaggtcg gcgaacgcct cgccgtcgtc ttccaccgcg
     2701 ccggcagagc ccgcgtcgac gacccacgcc cggacccggc ccccgaatgt ccccaggacg
     2761 aagcggtcga cgatcgcgac gtcgccgatc cgggcgacga gccggttgtc gtcggcttcg
     2821 agccgcaccc gcggcgggag caggtctcgc agcgcctcca agaagcgctc gccctcgggc
     2881 gtcgccgcta cgggggccgg gctttgctcg ccgccgcttc cgaacgcgat cccctcggtg
     2941 agctcgagcg cgtcgaccag ctcctgcagc tcggcacgct cctcttcggt gaggtgagag
     3001 aagtccggct cggctccacc catctcgagg tggtcgaggt acgccgcgag cgcgtcctgt
     3061 acccggtcgc tatccgccat gtcccgcacc tcctctccgt cctgcaccga gcgtcctatc
     3121 ttcgttgcgc ttgacgtatc tctgcagctt gatgaacgcc tccgccgtta tctgcccgac
     3181 cctctggccg gtcagcggcg ggtcgagctc gcgtccgacc gccgcccggc tcttgccgtc
     3241 gatccgaacc gccacgaaca cgcggcgctg gagctcggtg agaagccccg gctcgtggat
     3301 cgcctcctcc cacagagcga ggcggccgac ggcgccgatc tgctcccggc tgcggaagta
     3361 gtcgacgtcc gcaccgatcg agcccggcac ctcctcggcc tccggcaggt cgatcgatcg
     3421 atccctgcgc cgcgcctcga cccgccgctg gtggtcgatg gccttgttgc gcgccaccgt
     3481 gagcgcatgg ggcaagaagt cgcccttcgt cgcgaacccc tgctcggcca tcgccgccca
     3541 ggcctcgtcg acgacgtccc acgggtcgac ccccgcgatc acctcgctgt tcgaccgcca
     3601 cgtcctcagc accgaagcgc gcatgagcct gcggatccgt ctgtatccgt catcgtcgat
     3661 cccgtcggga agcctcggtt gccgagggcg tgcctccacg tggtgtcttt atcgcagggg
     3721 gcggaaaacc gaaaggaccc gttgcggtgg atgatgctga cccgatggcc gccaccgaag
     3781 agacgctcga gatcgcgggg cgcgaggtcc ggatcacgag ccccgagaag gtcttcttcc
     3841 cggagcgagg ggagacgaag ctcgacctcg tccgccccta cctgcggttc gaggagccgg
     3901 tgatgcgcac gatggggggc cggcccctgc tgttgcagcg gttcccgaac ggggcgacgg
     3961 gatcgtcgtt cttccagaag cggattccc
//
LOCUS       DASQDO010000323         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1013338, whole genome shotgun sequence.
ACCESSION   DASQDO010000323 DASQDO010000000
VERSION     DASQDO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013338"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VEV43_09095"
     CDS             <1..773
                     /locus_tag="VEV43_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HYP23716.1"
                     /translation="MVDDPELLDLVELEVRELLTEYEYPGDDIPVTRVSALKALDGDA
                     DAAKGILELMQTVDDYIPEPERDVNKPFLMPIEDVFTITGRGTVVTGRVEQGVLRVGE
                     EIEIVGIEEKTSKTVVTGIEMFRKMLDAAQSGDNAGILLRGTKKDEVQRGQVLCKPGS
                     ITPHTKFQAQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSVQLPGGSEMVMPGDN
                     TEMEVTLIQPVAMDEGLRFAIREGGRTVGAGRVTKILE"
     gene            796..1095
                     /gene="rpsJ"
                     /locus_tag="VEV43_09100"
     CDS             796..1095
                     /gene="rpsJ"
                     /locus_tag="VEV43_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HYP23717.1"
                     /translation="MKIRIRLKAYDHEVIDQSAKKIVETVTRTGASVNGPIPLPTERS
                     VYTVIRSPHKDKDSREHFEMRTHKRLLDIVNPTPKTVDSLQRLDLPAGVDIELKL"
     gene            1118..1783
                     /gene="rplC"
                     /locus_tag="VEV43_09105"
     CDS             1118..1783
                     /gene="rplC"
                     /locus_tag="VEV43_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HYP23718.1"
                     /translation="MAEVKGILGRKIGMTQVFDENGRAVPVTVVEAGPCRVAQVKTPE
                     TDGYAAIQLAFGEPKRTNKPAAGHFAKAGVDPARHLVELRLDELGDMAPGVEIRADVF
                     EPGEVVDVVGVTKGKGFAGGMKRHNFAGLSGSHGTHKKHRSPGSIGGASTPARVFKGQ
                     RMAGHMGHVRVTTLNLVVIKADAERNLILIRGAVPGPKDGIVMIRSAIRLRRRGGTTR
                     KAS"
     gene            1783..2427
                     /gene="rplD"
                     /locus_tag="VEV43_09110"
     CDS             1783..2427
                     /gene="rplD"
                     /locus_tag="VEV43_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HYP23719.1"
                     /translation="MAFSAKVIDATGSTTGSRDLPDELFGGEVNVPLMHQAVTAQLAA
                     ARAGTASTKTRSEVRGGGKKPWRQKGTGRARHGSTRSPIWVGGGTVFGPKPRDHSVRM
                     PKKMRRAALRSALASKAGDGRVWILDGFTEAKTKAAAAALRAAGIEGRILVVLDAGAD
                     SSTVVDRAFRNLDKAAFSLGTALGTYDVLVADHVIFTSDAYDAYTKKLSEGATV"
     gene            2424..2723
                     /gene="rplW"
                     /locus_tag="VEV43_09115"
     CDS             2424..2723
                     /gene="rplW"
                     /locus_tag="VEV43_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HYP23720.1"
                     /translation="MSTKSDPRDVILEPVVSEKSYSLLDQNSYTFVVHPDANKTEIRQ
                     AVETIWGVRVLSVNTLNRKGKLKRFRFVQGRRPNSKRAIVKLAEGDKIEIFETQG"
     gene            2734..3564
                     /gene="rplB"
                     /locus_tag="VEV43_09120"
     CDS             2734..3564
                     /gene="rplB"
                     /locus_tag="VEV43_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HYP23721.1"
                     /translation="MPIRKAKPTSPGRRFQSHSDFSDVTRSEPEKSLLEPKPKKAGRN
                     SYGRVTARHQGGGNKVRYRRIDFRRDKDGVPAKVAHVEYDPNRNARIALLHYKDGEKR
                     YILMPNRVKVGDVIQSGPGADIRPGNALPLRNIPVGTVVHNVELRPGAGGKMARSAGA
                     SVQLVAKEGTNATLRLPSGEMRMVPISCRATVGEVGNAEAELISIGKAGRARWKNKRP
                     SVRGVAMNPVDHPLGGGEGKSSGGRHPVSPWGKKEGRTRKSKPSDAMIVRRRGKKRGR
                     "
     gene            3570..3854
                     /gene="rpsS"
                     /locus_tag="VEV43_09125"
     CDS             3570..3854
                     /gene="rpsS"
                     /locus_tag="VEV43_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HYP23722.1"
                     /translation="MPRSLKKGPFVDDHLLLKVDAMNKRNEKRVIKTWSRRSTVIPDM
                     VGHTIAVHDGRKHVPVYVTESMVGHKLGEFAPTRAFRTHGGSAERPTRVR"
     gene            3854..>4164
                     /gene="rplV"
                     /locus_tag="VEV43_09130"
     CDS             3854..>4164
                     /gene="rplV"
                     /locus_tag="VEV43_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HYP23723.1"
                     /translation="MARKIAPDAQEAIATARYIRISPTKARQVVDLIRGRHVDDARRV
                     LRFNERGASTTVGKVLASAVANAQHNDELDPEELFVKACFADEGPTLKRFTPRARGRA
                     GR"
BASE COUNT          774 a         1416 c         1430 g          544 t
ORIGIN      
        1 acatggtcga cgaccccgag ctcctcgacc tcgtcgagct cgaggtgcgc gagctcctca
       61 ccgagtacga gtacccgggc gacgacatcc ccgtcacgcg cgtctccgcg ctgaaggcgc
      121 tcgacggcga cgccgacgcg gccaagggga tcctcgagct catgcagacc gtggacgact
      181 acatccccga gcccgagcgt gacgtgaaca agccgttcct catgccgatc gaggacgtct
      241 tcacgatcac cggccgtggc acggtcgtca ccggccgcgt cgagcaaggc gtgctcaggg
      301 tcggcgagga gatcgagatc gtcggtatcg aggagaaaac ctcgaagacc gtcgtcaccg
      361 gcatcgagat gttccgaaag atgctcgacg cggcgcagtc cggcgacaac gcgggaatcc
      421 tgctgcgagg cacgaagaag gacgaggtcc agcgcggcca ggtcctctgc aagcccggct
      481 cgatcacgcc gcacacgaag ttccaggcgc aggtctacat cctctcgaag gacgagggtg
      541 gccgccacac gccgttcttc aacaactacc ggccgcagtt ctacttccgc accaccgacg
      601 tgacgggctc ggtccagctc ccgggcggct ccgagatggt catgccgggc gacaacaccg
      661 agatggaggt cacgctgatc cagcccgtgg ccatggacga gggacttcgc ttcgcgatcc
      721 gtgaaggtgg ccgcaccgtc ggcgccggcc gcgtgacgaa gatcctcgag taaggacgaa
      781 tacgtggcga acggaatgaa gatcaggatc aggctgaagg catacgacca cgaggtcatc
      841 gaccaatcgg ccaagaagat cgtcgagacg gtgacccgta ccggtgccag cgtcaacggc
      901 ccgatcccgc tgccgaccga gcgcagcgtc tacacggtca tccgctcgcc gcacaaggac
      961 aaggactcgc gcgagcactt cgagatgcgt acgcacaaga ggctgctcga catcgtcaac
     1021 ccgacgccga agacggtcga ctcgctccag cggctcgacc ttcccgccgg cgtcgacatc
     1081 gagctcaagc tctaacgaaa agaagaggag aacctggatg gcggaggtga aaggcatcct
     1141 cggtcgcaag atcgggatga cccaggtctt cgacgagaac gggcgcgccg tgcccgtgac
     1201 cgtcgtcgag gccgggccct gccgcgtcgc gcaggtgaag accccggaga ccgacgggta
     1261 cgccgcgatc cagctcgcct tcggcgagcc gaagcggacg aacaagccgg ccgcgggcca
     1321 cttcgccaag gcgggcgtcg accccgcgcg gcatctcgtc gagctgcggc tggacgagct
     1381 cggcgacatg gcgccgggcg tcgagatccg cgcggacgtg ttcgagccgg gcgaggtggt
     1441 cgacgtcgtc ggcgtcacca agggcaaggg gttcgccggc gggatgaagc gtcacaactt
     1501 cgccgggctc tccggcagcc acgggacgca caagaagcac cgttcccccg gctcgatcgg
     1561 cggcgcgtcg acgccggcgc gcgtcttcaa gggacagcgc atggccggcc acatgggcca
     1621 cgtccgggtc acgaccttga acctcgtcgt gatcaaggcc gacgcggagc ggaacctgat
     1681 cctgatccgc ggcgcagttc ccgggccgaa ggacggcatc gtgatgatcc gttcggcgat
     1741 ccggctccgc cgccgcggtg gcacgactag gaaggcgagc tgatggcgtt cagcgcgaag
     1801 gtcatcgacg cgaccggcag cacgaccggt tcgagggacc tgcccgacga gctcttcggc
     1861 ggcgaggtca acgtgccgct gatgcaccag gccgtcacgg cccagctggc cgcagcccgc
     1921 gccgggacgg cgtccaccaa gacgcggtcc gaggtgcgcg gcggcgggaa gaagccctgg
     1981 cgacagaagg gcacgggacg cgcccggcac ggctcgacgc gtagcccgat ctgggtcggc
     2041 ggtggcacgg tcttcgggcc gaagccgcgc gaccactcgg tgcggatgcc gaagaagatg
     2101 cgccgcgccg ctctgcggtc ggcgctcgcc tcgaaggccg gcgacggccg ggtctggatc
     2161 ctcgacgggt tcaccgaggc gaagacgaag gccgcggccg cggctctgag ggccgccggc
     2221 atcgaggggc gcatcctcgt ggtgctcgac gccggcgcgg acagctcgac ggtcgtcgac
     2281 cgtgccttcc gcaacctcga caaggcggcg ttctcgctcg ggaccgcgct cggcacctac
     2341 gacgtgctcg ttgcggacca cgtgatcttc acgtcggacg cctacgacgc ctacacgaag
     2401 aagctgagcg agggggccac ggtatgagca ccaagagcga tccccgggac gtgatcctcg
     2461 agcccgtcgt cagcgagaag agctattcgc tgctcgacca gaactcgtac acgttcgtcg
     2521 ttcaccccga cgcgaacaag accgagatcc gccaggccgt cgagacgatc tggggggtcc
     2581 gggtcctgtc cgtcaacacg ctcaaccgca agggcaagct caagcggttc cggttcgtcc
     2641 agggccgccg cccgaactcg aagcgagcga tcgtcaagct cgccgagggc gacaagatcg
     2701 agatcttcga gacgcagggt tagggagtca tagatgccga tccgtaaagc gaagccgaca
     2761 tccccggggc gcaggttcca gtcgcactcc gacttctcgg acgtcacgcg ctccgagccg
     2821 gagaagtcgc tgctcgagcc gaagccgaag aaggccggga ggaactccta cggccgcgtg
     2881 acggcgcgcc accagggtgg cggcaacaag gtccgctacc gccggatcga cttccgccgc
     2941 gacaaggacg gcgtgccggc gaaggtcgcg cacgtcgagt acgacccgaa ccgcaacgcc
     3001 cgcatcgcgc tgctccacta caaggacggc gagaagcgct acatcctcat gccgaaccgc
     3061 gtgaaggtgg gcgacgtcat ccagtcgggg cccggggcgg acatccggcc cggcaacgcg
     3121 ctgccgctgc gcaacatccc ggtcggtacc gtcgtgcaca acgtcgagct ccggcccggc
     3181 gccggcggca agatggcccg ctccgctggg gcgtcggtcc agctcgtcgc gaaggagggg
     3241 accaacgcca cgcttcgcct cccctccggc gagatgcgga tggtcccgat ctcgtgccgc
     3301 gcgacggtcg gcgaggtcgg caacgcggag gccgagctga tctccatcgg caaggccggg
     3361 cgcgcccgct ggaagaacaa gcgcccgtcg gtgcgcggcg tcgcgatgaa cccggtcgac
     3421 caccccctcg gtggtggcga gggcaagtcg tccggaggtc ggcaccccgt cagcccgtgg
     3481 ggcaagaagg aaggccgcac gcgtaagtcc aagccgtccg acgccatgat cgtccggcgg
     3541 cgagggaaga agagaggtcg gtagcagcca tgccgcgaag cttgaagaag ggcccgttcg
     3601 tcgacgacca cctgctcctc aaggtggacg cgatgaacaa gcgcaacgag aagcgcgtga
     3661 tcaagacttg gtcccgccgc tcgacggtga tccccgacat ggtcggccac acgatcgccg
     3721 tccacgacgg gcgcaagcac gtcccggtct acgtcaccga gtcgatggtc gggcacaagc
     3781 tcggcgagtt cgcgcccacg cgcgcgttcc gcacgcacgg tggctccgca gagcgcccga
     3841 cgagggtgag gtaatggcga ggaagatcgc tcccgacgca caggaggcca tcgcgacggc
     3901 gcggtacatc cgcatctcgc cgaccaaggc ccgccaggtg gtggacctca tccgcggccg
     3961 tcacgtggac gacgcccggc gcgtgctgcg cttcaacgag cgcggcgcct cgaccacggt
     4021 cggcaaggtc ctcgcgtccg cggtggcgaa cgcacagcac aacgacgagc tcgatcccga
     4081 ggagctcttt gtgaaggcgt gcttcgccga cgagggtccg acgctgaagc ggttcacccc
     4141 tcgcgctcgc ggccgtgcgg gtcg
//
LOCUS       DASQDO010000324         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_34486, whole genome shotgun sequence.
ACCESSION   DASQDO010000324 DASQDO010000000
VERSION     DASQDO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34486"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VEV43_09135"
     CDS             complement(<1..398)
                     /locus_tag="VEV43_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HYP23724.1"
                     /translation="MSISERYARVLDRVARAAQSAGRAPEDVTLVAVSKTWPADVLKD
                     AAGAGVEVFGENRAQELKEKFAVLGDRVRWHFVGPLQSNKVRNVVGVAELVHSVDRYG
                     LGEAIARRARSLGIVQDVLVEVNTGGEATK"
     gene            complement(395..1135)
                     /locus_tag="VEV43_09140"
     CDS             complement(395..1135)
                     /locus_tag="VEV43_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HYP23725.1"
                     /translation="MDFQPLLHEHEDDGVVWLADDGARELGVLVAFSARLGGVSRAPY
                     DTLNLAVRVGDDRGDVMENRRRAGAAAGFDAQRLALALQVHAADVIDVGASDAGVLGE
                     ADGLFTEARGPVLGILAADCAPVALWGSRGVALVHAGWRGLVAGVVAKGVEKVGEVHA
                     AWVGPSIHACCYEGGPEVVAGFTAAGLPVDDPSHVDPGRAAGAALNAAGVERVVVSDV
                     CTHCDERVISYRRDGVTGRHGAFVSLLA"
     gene            complement(1206..1652)
                     /locus_tag="VEV43_09145"
     CDS             complement(1206..1652)
                     /locus_tag="VEV43_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23726.1"
                     /translation="MKKLIVTSIVAALVAGAMVAPVEAKKKKAKPRVAESTYANPAVG
                     VPGVVGSPAAGGAFEFGTLSTENFVVVDITDDGGQAVTFTMSQDSDPANTGYEIMGTW
                     CGTTEEPVQITPGLPLRVTVYTTPGPDQPSCTGPATSGTMTATFTR"
     gene            complement(1782..>2732)
                     /gene="ftsZ"
                     /locus_tag="VEV43_09150"
     CDS             complement(1782..>2732)
                     /gene="ftsZ"
                     /locus_tag="VEV43_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYP23727.1"
                     /translation="VKLDIGRDLTRGLGAGADPEVGRQAAEEHREEIEEVLKGADMVF
                     ITAGKGGGTGTGGAPIVAEIAKSLGALTIGVVTRPFGFEGRQRATKAEHGIANLKDKV
                     DTLIVIPNDRLLDVGDATTSVLEAFRMADEVLLQGVQGITDLITTPGLINLDFADVKA
                     VMTDAGSSLMGIGQARGDNRAAEAARLAISSPLLEASIDGARGVLLNIAGGSDLGLFE
                     VNEAAGIISQAAHQDANIIFGAVVDDTLGDEVRVTVIAAGFDRWGHREAEDLVKPEEE
                     IFSIPAVDDSEPAFLGETERERLVFDAEDDLDIPSFLRNE"
BASE COUNT          366 a          986 c          956 g          424 t
ORIGIN      
        1 tgcttggtcg cctctccccc ggtgttcacc tcgacgagca cgtcctgcac gatccccagg
       61 gatcgtgcgc gccgggcgat cgcctcgccg agcccgtagc ggtcgacgga atgcacgagc
      121 tcggccacgc cgacgacgtt acggaccttg ttcgactgga ggggcccgac gaagtgccag
      181 cggaccctgt cgccgagaac ggcgaacttc tccttgagct cctgcgcgcg gttctcgccg
      241 aagacctcga cgccggcgcc ggccgcgtcc ttgagcacgt cggcaggcca cgtcttcgac
      301 acggcgacga gcgtgacgtc ctcgggagcg cggcccgcgc tctgcgcggc tcgggcgacg
      361 cggtccagca cgcgcgcgta cctctcggag atgctcacgc gagcaacgat acgaacgcgc
      421 cgtgcctgcc ggtgacgccg tcgcgccggt acgagatgac gcgctcgtcg caatgggtgc
      481 acacgtcgct cacgacgacg cgctctacac cggccgcgtt cagcgcggcc cccgcggcgc
      541 ggcccggatc cacgtgcgag gggtcgtcga ccgggagccc cgcagccgtg aacccggcca
      601 cgacctcggg cccgccctcg tagcagcacg cgtggatcga cgggccgacc cacgccgcgt
      661 gaacctcgcc caccttttcg actcccttgg cgacgacgcc ggccacgagc ccgcgccaac
      721 ccgcgtgaac gagcgcgacc ccgcggctgc cccacagcgc gacgggcgcg cagtcggcgg
      781 cgaggatccc cagcacgggg ccgcgcgcct cggtgaacag accgtcggcc tcgccgagca
      841 cgcccgcgtc gctcgcgccg acgtcgatca cgtcggccgc gtggacctgg agcgcgaggg
      901 ccagccgctg tgcgtcgaag ccggccgcgg cccccgcgcg gcggcggttc tccatgacgt
      961 cgccgcggtc gtcgccgacg cgcacggcca ggttcaatgt gtcgtacggg gcccggctga
     1021 cgcccccgag ccgcgcgctg aacgcgacga gcacgccgag ctcacgggcg ccgtcgtcgg
     1081 cgagccacac gacgccgtcg tcttcgtgct cgtgcaataa cggttggaag tccacggacc
     1141 cagtgtggcc cagacgcgca accgccccct actagagggg gcggttgtct tgagtccttc
     1201 gtcggttacc tggtgaacgt cgccgtcatg gtcccagagg tggcggggcc ggtgcacgac
     1261 ggctggtcgg ggccgggcgt cgtgtagacg gtcactcgca gcgggaggcc gggcgtgatc
     1321 tggaccggct cctcggtcgt cccgcaccac gtgcccatga tctcgtagcc ggtgttggcg
     1381 gggtcgctgt cctgcgacat cgtgaacgtg acggcctggc cgccgtcgtc ggtgatgtcg
     1441 acgacgacga agttctcggt gctgagcgtc ccgaactcga acgcaccgcc ggcggcgggg
     1501 ctgccgacga cgccggggac gccgactgcc gggttcgcgt aggtcgactc ggcgacacgc
     1561 ggcttcgcct tcttcttctt ggcctcgacg ggcgcaacca tggcgcctgc gacgagagca
     1621 gcaacgatgc tggtgacgat gagcttcttc atctcggtgg ccccccagtg gaaagtccgc
     1681 tgtgtcttgg ggtgtgtttt ctccggcgcg gggggttctc ctgcgggggc cggacgcgaa
     1741 ggaggcggcc cccgagggcc gcctcctggt ggtcgctctc cttattcgtt gcgcaggaac
     1801 gacgggatgt cgaggtcgtc ctcggcgtcg aagaccaggc gctcgcgctc ggtctcgccc
     1861 aggaacgcgg gctccgagtc gtcgaccgcc gggatcgaga agatctcctc ctccggcttg
     1921 accaggtcct cggcctcgcg gtggccccag cggtcgaacc ccgccgcgat caccgtcacg
     1981 cgcacctcgt cgccgagcgt gtcgtcgacg accgcaccga agatgatgtt ggcgtcctgg
     2041 tgggcggcct gcgagatgat ccccgccgcc tcgttgacct cgaacaggcc gaggtccgag
     2101 ccaccggcga tgttgagcag gaccccgcgg gccccgtcga tcgacgcctc gaggagcgga
     2161 gaagagatcg cgagccgtgc ggcctcggcg gccctgttgt caccgcgggc ctggccgatc
     2221 cccatgaggg acgagccggc gtcggtcatg acggccttca cgtcggcgaa gtcgaggttg
     2281 atcagccccg gggtcgtgat gaggtcggtg atcccctgga cgccctggag caggacctcg
     2341 tcggccatcc ggaacgcctc gagcaccgac gtcgtcgcgt cgccgacgtc gagcagccgg
     2401 tcgttcggga tcactatgag cgtgtcgacc ttgtccttga ggttcgcgat gccgtgctcg
     2461 gccttggtgg cgcgctgccg gccctcgaag ccgaacggcc gggtgacgac cccgatcgtg
     2521 agggcgccga gggacttcgc gatctcggcc acgatgggtg cgccgccggt gccggtgccg
     2581 ccgcccttgc cggcggtgat gaacaccatg tcggcgccct tcaggacctc ttcgatctcc
     2641 tcgcggtgct cctccgctgc ctgacgcccc acctcggggt cggctcctgc gccgagtccc
     2701 cgcgtgagat ctcgcccgat gtcgagcttg ac
//
LOCUS       DASQDO010000325         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_647047, whole genome shotgun sequence.
ACCESSION   DASQDO010000325 DASQDO010000000
VERSION     DASQDO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647047"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VEV43_09155"
     CDS             complement(<1..619)
                     /locus_tag="VEV43_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HYP23728.1"
                     /translation="MTPGDDRSPRRPVRRAAFFDLDKTLIPGSSLFLLARGAYARDMF
                     RVRDLLRFAWGQLVFRLVGERASNTEMSRTSTLEFVTGHKAEELVAWGRQIAEERIMP
                     RVYEDIVRVIEEHKARGDLTFLVTAAPVELASTIGEGLEMTGTIATVSEVDSAGYYTG
                     RLVGPVMHGPEKAKAVAEVAAEHGIDLAECAAYSDSMNDLPLLESV"
     gene            complement(616..1167)
                     /locus_tag="VEV43_09160"
     CDS             complement(616..1167)
                     /locus_tag="VEV43_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HYP23729.1"
                     /translation="MDAIEAIMTRRSVPRCDGDVGRDAIETILAAAVRAPNHHLTQPW
                     RFVVLAGEARTELARAWAAGLEREGKDASRIPEKVLRSPVIVCVLERPHLDNPKVVEI
                     DEHYAVGAAIQNMLLAAHALGLGAMHRTGAATTMPEVRDYLGAADGELIAGFVYVGRP
                     PEGDDSRPRSRRTDHSEITEWRG"
     gene            1200..1982
                     /locus_tag="VEV43_09165"
     CDS             1200..1982
                     /locus_tag="VEV43_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYP23730.1"
                     /translation="MLVGAMNFPGRSVLKEIHRIAEDRFDFVDLTLEPPAAWFPDGKE
                     VGRLLGDLGLSAVGHTPYYLPIASPFPELRTVARDLFRRACDVFADAGVELVNVHPDQ
                     RVPLQSADKVRSANADAIAALAEDAASRGIRLMVENLDNRFSRVEDFEEIFRAAPDAG
                     FHLDVGHANLRLGLGEPNRTPALLKAYGDRLAHVHLSDNPGGTEDRHLPLGAGNIDWK
                     TVVRWLKDAGYDGTVTLEVFSRTREHLRTSRKLWLGWWTERD"
     gene            2035..2205
                     /locus_tag="VEV43_09170"
     CDS             2035..2205
                     /locus_tag="VEV43_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23731.1"
                     /translation="MSLKRVMWFLLIGFGVFFLIESPGEAARLVKITGESAGEWLSTA
                     ADSLSKFVRSLV"
     gene            2210..2779
                     /locus_tag="VEV43_09175"
     CDS             2210..2779
                     /locus_tag="VEV43_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYP23732.1"
                     /translation="MVTGPGRYLASDEHVVLQTRRHVAVLARPAFTALGALALGLFVG
                     LSASPGDGGDLLDTIAGLFALWFLLRFMWRAWEWWVDRIFVTEKRIFEVSGLLTRKVA
                     SMPLGNLTDMTYQRSLWGRIFGFGELIVETAGQAQALSRIAYLSNPDDFYRAVTALVT
                     ASLLPSASAPEVMAEQDEDDTGPLPRVIV"
     gene            2853..3377
                     /locus_tag="VEV43_09180"
     CDS             2853..3377
                     /locus_tag="VEV43_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23733.1"
                     /translation="MKKIVTLGAAAVLAGSLLPGSATAAKPKQQQVSGSIALPAPFTD
                     DTGCFAGLQRRVAIVSQEQVNGVIGYHFDVDPATVGKPFVLEATGGQGDVDLDITFYY
                     EFGTIEDVTGDPQNAGSPVNYSFASREPGGESGVIPKGDYTKAIVCVYGGQQGAGAAA
                     TFDYVAGKGVKLPK"
     gene            3463..>3654
                     /locus_tag="VEV43_09185"
     CDS             3463..>3654
                     /locus_tag="VEV43_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23734.1"
                     /translation="MTTAIIASFAAVVALGFLEGVGRFYPARKTWWRLRRTRGRSAVV
                     LMRARCERAADPGLARRLAS"
BASE COUNT          520 a         1317 c         1249 g          568 t
ORIGIN      
        1 ctaccgactc gagcagcggg aggtcgttca tcgagtccga gtaggcggcg cactcggcga
       61 gatcgatccc gtgctctgcc gcgacctcgg cgacggcctt cgccttctcc ggcccgtgca
      121 tcacggggcc gacgagccgg cccgtgtagt agccggcgga gtcgacctcg gacaccgtcg
      181 cgatcgtgcc cgtcatctcg agcccctcgc cgatcgtcga cgcgagctcg acgggggccg
      241 cggtcacgag gaacgtgagg tcgccgcgcg ccttgtgctc ctcgatcacg cggacgatgt
      301 cctcgtagac gcgcggcatg atgcgctcct cggcgatctg ccggccccac gcgacgagct
      361 cctcggcctt gtgcccggtc acgaactcca gcgtcgacgt gcgcgacatc tccgtgttgc
      421 tcgcgcgctc gccgaccagc cggaagacca gctgtcccca cgcgaaccgc agcaggtcgc
      481 gtacccggaa catgtcccgc gcgtacgcac cgcgcgcgag caggaacagg ctcgaccccg
      541 ggatcagcgt cttgtctagg tcgaagaacg cggcccgccg gaccggtcgc ctcggcgagc
      601 ggtcgtcacc aggagtcatc cgcgccactc cgtgatctcg gagtggtccg tccggcgcga
      661 ccggggccgc gagtcgtcgc cctcgggagg acgcccgacg tagacgaatc ccgcgatcag
      721 ctcgccgtcc gcggccccca ggtagtcgcg cacctccggc atcgtcgtcg cggcccccgt
      781 ccgatgcatc gcgccgagcc cgagcgcgtg cgccgcgagc agcatgttct ggatcgccgc
      841 gccgaccgcg tagtgctcgt cgatctcgac gaccttcggg ttgtcgaggt gcgggcgttc
      901 cagcacgcag acgatcacgg gggagcgcag caccttctcg gggatgcggg aggcgtcctt
      961 gccctcccgt tcgagccccg ccgcccaggc gcgggcgagc tcggtgcggg cctcgccggc
     1021 cagcacgacg aagcgccagg gctgcgtcag atggtggttc ggtgccctca cggccgcggc
     1081 gaggatcgtc tcgatcgcgt cgcgcccgac gtcgccgtcg cagcggggga cggagcgccg
     1141 cgtcatgatc gcttcgatcg catccacggt ccgaggatag gggtccgtat catcggccgg
     1201 tgttagtcgg agccatgaac ttccccgggc gctcggtcct caaggagatc caccggatcg
     1261 cggaggatcg cttcgacttc gtcgacctca cgctcgagcc cccggcggcg tggttccccg
     1321 acggcaagga ggtcggccgg ctcctcggcg acctcggcct cagcgccgtc ggccacaccc
     1381 cctactacct gccgatagcg tcgccgttcc cggagctgcg gacggtcgca cgtgacctct
     1441 tccggcgtgc gtgcgacgtc ttcgccgacg ccggggtcga gctcgtcaac gtccatcccg
     1501 accagcgagt cccgttgcag tccgccgaca aggtgcggag cgcgaacgcc gacgcgatag
     1561 cggcgctcgc cgaggacgcg gcctcgcggg gcatccggct gatggtcgag aacctcgaca
     1621 accgcttctc ccgggtggag gacttcgagg agatcttccg cgcggccccc gacgcgggct
     1681 tccacctcga cgtgggccac gcgaacctgc ggctcggcct cggcgagccg aaccgcaccc
     1741 cggccctact gaaggcgtac ggggaccgcc tcgcgcacgt acacctctcg gacaaccccg
     1801 gcgggaccga ggaccggcac ctgcccctgg gggccgggaa catcgactgg aagacggtgg
     1861 tccgctggct gaaggacgcc ggctacgacg ggaccgtcac gctcgaggtc ttctcccgca
     1921 cccgggaaca cctccgcacc tcgcgcaagc tctggctcgg ctggtggacg gagcgcgact
     1981 gacgagaggc cgaatccgca cgaaccaccc ctagggccct atgatgtgtt gctaatgagc
     2041 ctaaagaggg tcatgtggtt cctgttaatc gggttcgggg tcttcttcct gatcgagtcc
     2101 cccggcgagg ccgcgcgcct cgtgaagatc accggtgaga gcgcggggga gtggctgtcc
     2161 acggccgccg acagcctctc gaagttcgtc aggtccctcg tctagggccg tggtcaccgg
     2221 ccccggccgc tacctcgcct cggacgagca cgtcgtcctc cagacgcggc gccacgtagc
     2281 ggtcctcgcc cgtccggctt tcacggcgct cggcgcgctc gcgctcggtc tgttcgtagg
     2341 cctgagcgcg agccccggcg acgggggcga cctcctggac acgattgccg gcctgtttgc
     2401 gctgtggttc ctcctccgct tcatgtggcg ggcctgggag tggtgggtcg accggatctt
     2461 cgtcaccgag aagcggatct tcgaggtctc cggtctcctg acccgcaagg tggcctcgat
     2521 gccgctcggc aacctcacgg acatgaccta ccagcggtct ctctgggggc ggatcttcgg
     2581 gttcggcgag ctgatcgtcg agaccgccgg gcaggcccag gccctcagcc ggatcgcata
     2641 cctgtcgaac cccgacgact tctaccgggc cgtcacggcg ctggtgacgg cgagcctcct
     2701 gccgtcggcc tcggccccgg aggtcatggc ggagcaggac gaggacgaca ccgggccgct
     2761 gccccgcgtg atcgtctgaa cctccgggca ggagcatggc ccctcggggc cgaacctccc
     2821 agtagtcgaa cttgctaccg gaggtaaatg ccatgaagaa gatagtcacg ctgggtgcag
     2881 ccgccgtgct ggcgggttcg ctcctcccgg gcagcgccac cgcggcgaag cccaagcagc
     2941 agcaggtgtc cggctcgatc gctctgccgg cgccgttcac ggacgacacc gggtgcttcg
     3001 cggggctcca gcgccgcgtg gcgatcgtca gccaggagca ggtcaacggg gtcatcggct
     3061 accacttcga cgtcgacccc gcgacggtcg gcaagccgtt cgtcctcgag gcgaccggtg
     3121 gccagggtga cgtcgacctg gacatcacct tctattacga gttcgggacc atcgaagacg
     3181 tcaccggcga cccgcagaac gcaggctccc cggtcaacta ctcgttcgct tcgcgggagc
     3241 cgggcggcga gtccggcgtc attcccaagg gcgactacac gaaggcgatc gtctgcgtgt
     3301 acggcggcca gcagggtgcg ggcgccgcgg cgacgttcga ctacgtggcc ggcaagggcg
     3361 tgaagctccc gaagtaacga tcgacccgaa gttcactcgg aggggcgggc gaccgcccct
     3421 ccggcgcgtc cgggatcttt cccgggtcgt agccttgggc gcgtgacgac cgcgatcatc
     3481 gcctcgttcg ccgccgtggt ggccctgggc ttcctcgaag gcgtcggccg cttctacccg
     3541 gcccgcaaga cgtggtggag gctgcgccgg acgcggggcc gcagtgccgt cgtcctcatg
     3601 cgggcgcgct gcgagcgagc cgcggacccc ggcctcgctc gccgtctcgc gtcc
//
LOCUS       DASQDO010000326         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_585816, whole genome shotgun sequence.
ACCESSION   DASQDO010000326 DASQDO010000000
VERSION     DASQDO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585816"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VEV43_09190"
     CDS             <1..1190
                     /locus_tag="VEV43_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HYP23735.1"
                     /translation="VEGRLRLGRFEHTVALRGPRPYERVAAPSDFRRRFSQYDADTTV
                     WTNTTFSPDVPDVALVAARAFRQATGEKVDGVVLIDPRGIAALMPDGSSVRIRRTGAT
                     VGPEGLADYTYSTVYAELGGAKESRRRALLRLGRKAFEAILDGGALAARATVERAGSA
                     VAAGHIRIVSFDPDEQRVLDGLDASGDLDAAPVDSLLVAAHNFSADKLDYWARRSVSH
                     SCAIDDGDGARCTTSVTIENRAPAGLTRYVAGKPYGLLESLVEVYVPEAAEVEGVVLD
                     GEPAEFLSDRQDGHDVVGVFVETARGDRHEVAVTYGLPLDGGYSLEVLPQPLAHDAQL
                     RVAIDAPASWRVEGDGERGAGWLRYAGPLDRTMRFVAEPAGKTGLTAAWDALARFWRE
                     PLF"
     gene            complement(1197..1505)
                     /locus_tag="VEV43_09195"
     CDS             complement(1197..1505)
                     /locus_tag="VEV43_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23736.1"
                     /translation="MRALRVPIVIGLLFATLWLVGSAAAQPKPKYPPPRPSVLPTLIP
                     SSPPERQASTPPFTMPPPASEPPNVPFTGADVTLYAVIGVGLTAAGTTLVRVARRRSS
                     "
BASE COUNT          254 a          667 c          709 g          252 t
ORIGIN      
        1 ccgtcgaggg ccgcctgagg ctcggacgtt tcgagcacac cgtcgcgttg agggggccgc
       61 ggccctacga acgcgtcgcc gccccgtccg acttccgcag gcgcttctcg cagtacgacg
      121 ccgacacgac ggtctggacc aacacgacgt tctcgcccga cgtgcccgac gtcgcgctcg
      181 tggccgcgcg ggcgttccgc caggcgaccg gagagaaggt cgacggcgtc gtgctgatcg
      241 acccgcgcgg gatcgcggcg ctgatgcccg acgggtcgtc ggtccggatc cgcaggaccg
      301 gcgccacggt cggcccggaa ggcctcgcgg actacacgta ctcgacggtc tacgccgagc
      361 tcgggggcgc gaaggagtcc cggcggcgcg ccctgctgcg gctcggccgc aaggcgttcg
      421 aggcgatcct cgacggtggc gcgctcgcgg cacgggcgac ggtcgagcgc gccgggagcg
      481 ccgtagccgc ggggcacatc cgcatcgtct cgttcgaccc cgacgagcaa agggtgctcg
      541 acggcctcga cgccagcgga gacctcgacg cggcccccgt ggactcgctg ctcgtcgcgg
      601 cgcataactt cagcgccgac aagctcgact actgggcccg gcgctcggtg agccactcgt
      661 gcgcgatcga cgacggcgac ggtgcccggt gtacgacgtc ggtcacgatc gagaaccgcg
      721 cgccggccgg gctgacccgt tacgtggccg gcaagcctta cgggttgctc gagagcctcg
      781 tcgaggtcta cgtgccggag gccgccgagg tcgagggcgt ggtgctcgac ggggagccgg
      841 ccgagttcct gagcgaccgt caggacggcc acgacgtcgt cggggtgttc gtcgagaccg
      901 cccgcggcga ccggcacgag gtggcggtca cgtacgggct cccgctcgac ggcggctact
      961 cgctggaggt cctgccgcag ccgctcgctc acgacgcgca gctacgcgtg gcgatcgacg
     1021 cgcccgcgtc gtggcgggtc gagggcgacg gggagcgcgg agccgggtgg ctgcgctatg
     1081 cggggccgct ggacaggacg atgcggttcg tcgccgagcc ggccgggaag acgggcctca
     1141 ccgccgcgtg ggacgcgctc gcgaggttct ggcgcgaacc gttgttctaa gcggtcttag
     1201 gaagagcgtc gccgtgcgac gcgcacgagc gtcgtccccg ccgccgtaag cccgacaccg
     1261 atcacggcgt agagcgtgac gtccgcaccg gtgaacggga cgttcggcgg ctcggacgca
     1321 ggcggcggca tggtgaacgg aggcgtcgag gcctggcgct cgggcggcga cgacggaatg
     1381 agcgtcggca gcacggaagg ccggggcggg gggtacttcg gcttgggctg cgcggcggcc
     1441 gagccgacga gccacagggt cgcgaagaga agaccgatca cgatcggcac cctgagggcc
     1501 cgcatgcctg tctccttcct gacgatcggc ccgatgccgg cgtcgtacgt ccccgccacc
     1561 cacttcgggt ggacggttca catgataggc cctcccgggc cgcacctacg atacggcccc
     1621 cggcgtatgt ggaccgggtc cgccgggccc gctcgtcgcc gcgtatgccc aggtcggggg
     1681 ctcgctccgt cggcgcctac gattgggagc gtagacgccc ctcggcgggg cgccgccgca
     1741 ttcgtcctgg cagggattgg taatcagcgt cggtcggacc ataatgtcgc aatggtcacg
     1801 cgcccggccc cacctcccgc agtccggctc cgccggcggc tcgtgcagcc cgcacggccc
     1861 cccgtcccgg gcgcggcccg cg
//
LOCUS       DASQDO010000327         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1136511, whole genome shotgun sequence.
ACCESSION   DASQDO010000327 DASQDO010000000
VERSION     DASQDO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136511"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VEV43_09200"
     CDS             complement(<1..657)
                     /locus_tag="VEV43_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYP23737.1"
                     /translation="MSSDRLIETIRRSVIGDDEVIVGPYGPRRVTYADYTASGRALTF
                     IEDFIRDAVLPLYANTHTETSGTGLQTTRFREEARAIVCDAVGGSDEHAVIFCGSGST
                     GAIDKLVTVLELRLPRLLDDRYGLSERIPAAERPVVFIGPYEHHSNELPWRESIADLV
                     VIGEDANGMIDQAQLRDELERHRDRPLVIGSFSAASNVTGIVSDTDAISALLHEFGAL
                     S"
     gene            complement(913..1419)
                     /locus_tag="VEV43_09205"
     CDS             complement(913..1419)
                     /locus_tag="VEV43_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23738.1"
                     /translation="MPNGSQTATAKSSSDPRLELAYKEASRAITQQQASLSGLRTRAG
                     ALFGAANIATAFLAGVALENQDLVPVSWIATLCFVGVCGLTVSILLPRRGWVFEQNSR
                     VLVEEWIDEKDLGIDAIHKHLALFMRTHYERNQTSIDKLFRQYVWASLLLGVEIVAWL
                     FDLGQGVQ"
     gene            complement(1491..1961)
                     /locus_tag="VEV43_09210"
     CDS             complement(1491..1961)
                     /locus_tag="VEV43_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23739.1"
                     /translation="MITTSTGGFSRDRRHMRWLRVGLLVLAAMALLPGIWGTLWPRSF
                     FDDFPGAGNAWVAAFPPYNEHLVRDVGAFYLGYGVLLLGAALLTRRRLVQVALASWLV
                     ATIPHGIFHVRHLDVLDTTQAVLQSVILGVMVLLPLWLLWLSARMPWRTWSQTR"
     gene            complement(1972..>2526)
                     /locus_tag="VEV43_09215"
     CDS             complement(1972..>2526)
                     /locus_tag="VEV43_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP23740.1"
                     /translation="GVGNMGNNVYVLECPETHDAFVVDGCFEADTIIRGTEGANVVGI
                     LQTHGHMDHVQALPELVERLSVPGYAHPGDSYPVTIDRELSDGMTLHLGNRRIDVLHT
                     PGHTPGGVCFHSGRHLVSGDTLFPGGPGNTWGNADAFAQIIEAIESKLFVLPDDTFVY
                     PGHGDDTTIGAERPHLQEWKDRGW"
BASE COUNT          409 a          828 c          865 g          424 t
ORIGIN      
        1 cgacagtgca ccgaactcgt gcagcagcgc ggagatcgcg tccgtgtcgg agacgatccc
       61 ggtcacgttc gacgcggccg agaagctgcc gatcaccagc ggacggtcgc ggtggcgctc
      121 gagctcgtcc cgcagctgcg cctggtcgat catcccgttc gcgtcctctc cgatcacgac
      181 gaggtcggcg atcgactcgc gccacgggag ctcgttcgag tggtgctcgt aggggccgat
      241 gaacacgacc ggccgctcgg cggcggggat gcgctcggac aggccgtagc ggtcgtcgag
      301 caggcgcggg aggcggagct cgaggaccgt gacgagcttg tcgatcgcgc cggtcgagcc
      361 ggagccgcag aagatcaccg cgtgctcgtc gctgccgccg accgcgtcgc acacgatcgc
      421 gcgcgcctcc tctcggaagc gggtggtctg gagaccggtg cccgacgtct cggtgtgggt
      481 gttcgcgtac agcggcagga cggcgtcgcg gatgaaatcc tcgatgaacg tgagcgcgcg
      541 gccggacgcg gtgtagtcgg cgtaggtcac gcgccggggg ccgtaggggc cgacgatgac
      601 ctcgtcgtcg ccgatgaccg acctgcggat ggtctcgatg agacggtcgc tgctcatcgg
      661 gccaggctag cggggatcgc cggttggagc cggcgcgtcc cccttagcca tagtcgcctt
      721 cgtctaccag gggtcggagg gcttcttctc ctggcgttcg gacccggggt ctgaaggctc
      781 ctccgaggac gacctcagcg attcggttcg ctcgctcgcg ggatccggga tctcaccttg
      841 actgtcgctt cctgcatccg tcaactcgtc gtcctgctct tcgcgatcct ccactgcttc
      901 ctcctttcgt cgtcattgca ctccctgtcc aagatcgaac agccaggcca cgatctcaac
      961 gccgaggagg aggctggccc atacgtactg acgaaacagc ttgtcgatcg aagtttggtt
     1021 gcgctcgtaa tgcgtgcgca tgaacagggc gaggtgcttg tggatggcgt cgatacccaa
     1081 gtccttctcg tcgatccact cttcgacgag gacccgggag ttctgctcga acacccaacc
     1141 tcgccgaggg aggaggatgc tcacggtcaa accgcagact cctacgaaac aaagtgtggc
     1201 gatccagcta accggaacga ggtcttggtt ctcgagcgcg acgccggcga ggaacgcggt
     1261 ggcgatgttc gcggccccga agagagcccc cgcacgggtc ctgagcccag acagcgaggc
     1321 ctgctgttgc gtaatagcgc ggctcgcttc cttgtaagcg agctcgagac gtgggtcgga
     1381 agatgacttt gccgttgccg tctgggaccc gttcggcacg ctccgctcca cctcgattcg
     1441 tatctatagg taagcgactt tgtcacatag agaacggccc ggcaaggcct ctaccgggtc
     1501 tggctccacg tccgccacgg catgcgcgcc gacagccaga gcagccacaa cggtaggagg
     1561 accatcaccc caaggatgac cgactgcagc acggcttgcg tcgtgtcgag cacgtcgagg
     1621 tgccgcacgt ggaagatccc gtgcgggatc gtcgcgacga gccacgatgc gagcgccacc
     1681 tgtacgagcc gccgcctcgt caggagcgcg gcccccagga gcagcacgcc gtagccgagg
     1741 tagaaggccc cgacgtcgcg cacgaggtgc tcgttgtacg gcgggaacgc ggcgacccag
     1801 gcgttgccgg cgccggggaa gtcgtcgaag aacgagcgcg gccacagcgt gccccagatg
     1861 cccggaagaa gtgccatcgc ggcaagcacg aggagcccta cgcgcagcca gcgcatgtgc
     1921 cggcggtccc ggctgaagcc tcccgtcgag gtggtgatca cctcgttcgt tctaccagcc
     1981 gcggtccttc cactcctgga ggtgcggccg ctcggccccg atcgtcgtgt cgtcgccgtg
     2041 gccggggtag acgaacgtgt cgtcgggcag gacgaagagc ttgctctcga tcgcctcgat
     2101 gatctgcgcg aacgcatccg cgttgcccca cgtgttgccc ggcccccccg ggaacagcgt
     2161 gtcgccggac acgagatggc ggccggagtg gaagcagacg ccccccggcg tgtggcccgg
     2221 cgtgtgcagg acgtcgatcc ggcggttgcc gaggtggagc gtcatcccgt cggacagctc
     2281 gcggtcgatc gtgaccggat aggagtcgcc gggatgcgcg tagcccggca cgctcaggcg
     2341 ctcgacgagc tcgggcagcg cctgcacgtg gtccatgtgc ccgtgcgtct ggaggatgcc
     2401 gacgacgttc gcgccctcgg tcccgcggat gatcgtgtcg gcttcgaagc agccgtcgac
     2461 gacgaaggcg tcgtgcgtct cgggacactc cagcacgtag acgttgttgc ccatgttccc
     2521 gacgcc
//
LOCUS       DASQDO010000328         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_585986, whole genome shotgun sequence.
ACCESSION   DASQDO010000328 DASQDO010000000
VERSION     DASQDO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585986"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VEV43_09220"
     CDS             <1..194
                     /locus_tag="VEV43_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23741.1"
                     /translation="TGPAVRGEWATVTSHVRALEAAAPELVEPYRQATRLILRVAEGA
                     GKVTPEARRAAAAALGEPA"
     gene            191..1030
                     /gene="panC"
                     /locus_tag="VEV43_09225"
     CDS             191..1030
                     /gene="panC"
                     /locus_tag="VEV43_09225"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYP23742.1"
                     /translation="MRVARTVAEVRAFYRNERPRGTTVAFVPTMGALHDGHLSLVRKA
                     GEAADLVVVSIFVNPLQFGPGEDFESYPRDETRDVGLVEQEGVNLVFLPAVDQMYPEG
                     AVTTVHLDGPLATTLEGEVRPGHFDGVATVVAKLFNAIDPDVAVFGQKDAQQVAVIRR
                     MVRDLSYRTEIVVAPTVRDADGLALSSRNVYLSDDDREAATALHRALLAGEEAWRGGG
                     AEAAEDAMRGVLDGADGVEPDYAAAVDPYSFGAPTGDGPVLLAVAARVGRTRLIDNVL
                     VRP"
     gene            1042..1824
                     /locus_tag="VEV43_09230"
     CDS             1042..1824
                     /locus_tag="VEV43_09230"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HYP23743.1"
                     /translation="MLLAVDVGNSQTHLGVFNGRALAHEWRAFTDPRRTADELALLFG
                     QFLELADLSFSRQITGVVMSSVVPRATKNLREMTYKYFGFAPVVVEPKTKSGIDVTAY
                     TNPVGADRLANAVGAHDRWPDEDVVVVDFGTAITVDAISSDGKFLGGAIAPGVEAAAT
                     ALYASTALIAPVELKGPEVALGTNTSKGVEAGVVFGVAALADGLVERIVAEVGGAAHV
                     VATGGSAGLVVEHCRRIQTVDPILTLNGLRVIYDRNAGRADG"
     gene            1817..>3221
                     /gene="lysS"
                     /locus_tag="VEV43_09235"
     CDS             1817..>3221
                     /gene="lysS"
                     /locus_tag="VEV43_09235"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HYP23744.1"
                     /translation="MADHAPYPYSFERTAFAGDLHEQFGELEAGAESGKDVRVAGRVM
                     TTRSHGKIAFADLVDPSGRIQLFAQHAVLGDDGMEGFAQLTVGDIAGAEGQVVMTRRG
                     ELSVRVTDVVRLAPCLRPMPEKWHGLTDVEARYRQRYLDLIVNPDARRALEARAAATG
                     AIRRFFDERGFLEVETPLLQPIAGGAIARPFVTHHNALDMDLFLRIAPELFLKRLLIG
                     GAERVYEVNRSFRNEGVSTRHNPEFTMLEAYEAYVDYEHTMTLVEDLVRAIAEAVNGN
                     LELPVGERVIDLAQPFARKSMFEVIEEHTGAALLDAWNAEDADAIRNEARRLDVRIEE
                     AWAPGKVVAEIFEELVEKKLFEPTFVMGYPKEVSPLAKDHRSLAGFTEQADLILAGVE
                     IAPIYSELNDPDEQRRRFRQQAAARAAGDQEGMVPDEDFLEALAYGMPPAGGFGLGID
                     RLLTLLLGAPSIREVILF"
BASE COUNT          477 a         1089 c         1195 g          460 t
ORIGIN      
        1 tcaccggacc cgcggtgcgc ggcgagtggg cgaccgtcac ctcgcacgtg cgcgcgctcg
       61 aggcggcggc ccccgagctc gtcgagccct atcgccaggc gacccggctg atcctgcgcg
      121 tcgccgaggg ggccgggaag gtgacgcccg aggcgcggcg ggcggctgcg gccgcgttgg
      181 gggagccggc gtgagggtcg cgaggacggt cgccgaggtg cgcgcgttct accgcaacga
      241 acggccgcgc ggcacgaccg tggcgttcgt cccgacgatg ggggcgttgc acgacggtca
      301 cctgtctctg gtccgcaagg ccggcgaggc cgccgacctc gtggtcgtga gcatcttcgt
      361 gaacccgctg cagttcgggc cgggcgagga cttcgagtcg tatccgcgcg acgagacgcg
      421 cgatgtcggg ctcgtcgagc aggagggcgt caacctcgtg ttcctcccgg cggtcgacca
      481 gatgtacccc gagggagccg tgacgaccgt gcacttggac gggccgctcg cgacgacgct
      541 cgagggcgag gtgcggccgg gtcacttcga cggcgtcgcg acggtcgtgg cgaagctctt
      601 caacgcgatc gaccccgacg tcgccgtgtt cgggcagaag gacgcgcagc aggtcgcggt
      661 catccgccgg atggtgcgcg acctcagcta ccgcaccgag atcgtcgtag cgccgacggt
      721 gcgagacgcc gacgggctcg cgttgagcag ccgcaacgtc tacctgtccg acgacgaccg
      781 ggaggccgcc accgcgctgc accgggcgct tctcgccggc gaggaggcct ggcgcggagg
      841 gggagcggag gccgccgagg acgcgatgcg cggagtcttg gacggcgccg acggggtcga
      901 gcccgattac gccgcggccg tcgacccgta tagctttggg gcccccaccg gagacggccc
      961 cgtcctgctc gccgtcgcgg cccgggtggg gcgcacccgc ctgatcgaca acgtcctggt
     1021 ccggccctag aggagaacgc gttgcttctt gcggtagacg tgggcaactc gcagacgcat
     1081 ctcggcgtct tcaacgggcg cgcgctcgcg cacgagtggc gggcgttcac ggacccgagg
     1141 cgcactgcgg acgagctggc gctgctgttc ggtcagttcc tcgagctcgc cgacctctcg
     1201 ttctctcgcc agatcaccgg cgtcgtgatg tcgtcggtcg tgccgcgcgc gacgaagaac
     1261 ctgcgcgaga tgacctacaa gtacttcggc ttcgcacccg tggtcgtcga gccgaagacg
     1321 aagagcggga ttgacgtcac cgcgtacacg aacccggtgg gcgcggaccg gctcgcgaac
     1381 gccgtcgggg cgcacgaccg gtggcccgac gaggacgtcg tcgtcgtcga cttcggcacc
     1441 gcgataaccg tcgacgcgat ctcgtccgac ggcaagttcc tcggcggtgc gatcgccccg
     1501 ggcgtcgagg ccgcggcgac ggcgctctac gcgtcgaccg cgttgatcgc gcccgtcgag
     1561 ctgaagggtc cggaggtcgc gctcgggacg aacacgagca agggcgtcga ggccggggtc
     1621 gtcttcggcg tcgccgcgct cgcggacgga ctcgtcgagc ggatcgtggc cgaggtcggc
     1681 ggggccgcgc acgtggtcgc gaccgggggc tcggccgggc tcgtcgtcga gcactgccgg
     1741 cgcatccaga ccgtcgaccc gatcctcacg ttgaacgggc tgcgggtcat ctacgaccgc
     1801 aacgcggggc gagccgatgg ctgaccacgc gccgtacccg tactcgttcg agcgcaccgc
     1861 gttcgccggc gacctgcacg agcagttcgg cgagctcgag gcgggggcag agtcgggcaa
     1921 ggacgtgcgc gtcgcgggcc gcgtgatgac gacgaggtcc cacggcaaga tcgcgttcgc
     1981 cgacctcgtc gaccccagcg ggaggatcca gctcttcgct cagcacgcgg tcctcggcga
     2041 cgatgggatg gagggcttcg cgcagctcac cgtcggtgac atcgcggggg ccgagggcca
     2101 ggtcgtgatg acgcggcgcg gagagctctc cgtccgggtg accgacgtcg tgcggctcgc
     2161 gccgtgcctg cgtccgatgc ctgagaagtg gcacgggctc acggacgtcg aggcccgcta
     2221 ccgccagcgc tacctcgacc tcatcgtcaa cccggacgcg cgccgggccc tcgaggcacg
     2281 ggccgcggca accggcgcga tcaggcgctt cttcgacgag cgcggcttcc tcgaggtaga
     2341 gaccccgctg ctccagccga tcgccggcgg tgcgatcgcg cgcccgttcg tgacgcatca
     2401 caacgcgctc gacatggacc tcttcttgcg gatcgcgccg gagctgttcc tcaagcggct
     2461 gttgatcggg ggagccgaac gcgtctacga ggtgaaccgc agcttccgca acgagggcgt
     2521 gtcgacgcgc cacaaccccg agttcacgat gctcgaggcg tacgaggcat acgtcgacta
     2581 cgagcacacg atgacgctgg tcgaggatct cgtgcgcgcg atcgccgaag cggtcaacgg
     2641 gaacctcgag ctgcccgtgg gagagcgcgt gatcgacctc gcgcagccgt tcgcgcgcaa
     2701 gtcgatgttc gaggtgatcg aggagcacac gggggccgct ctgctcgatg cgtggaacgc
     2761 agaagacgcg gacgcgatcc ggaacgaagc gcggcgtctc gacgtccgaa tcgaagaggc
     2821 gtgggccccc ggcaaggtgg tcgcggagat cttcgaggag ctcgtggaga agaagctgtt
     2881 cgagccgacg ttcgtgatgg gctacccgaa agaggtctcg cccctggcga aggaccaccg
     2941 gtcgctggcc gggttcaccg agcaggcgga cctcatcctc gccggcgtcg agatcgcgcc
     3001 gatctactcc gagctcaacg accccgacga gcagaggcgg cggttccggc agcaggccgc
     3061 ggcgcgcgct gccggcgacc aggagggcat ggtccccgac gaggacttcc tcgaggccct
     3121 cgcgtacggc atgccgccgg cgggggggtt cgggctcggg atcgaccgcc tgctgacgct
     3181 gttgctcggc gcgccgagca tccgggaggt catcttgttc c
//
LOCUS       DASQDO010000329         5113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1013844, whole genome shotgun sequence.
ACCESSION   DASQDO010000329 DASQDO010000000
VERSION     DASQDO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013844"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VEV43_09240"
     CDS             complement(<1..448)
                     /locus_tag="VEV43_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23745.1"
                     /translation="MEDWLGVGIAVVSLGMSGVAMWWSRRGLKAFLLSDLHERIRQLK
                     AAALDHLDVDAGEYPTERDVEALRDRVMQLHVPWKDVRDATEQLIEECNGVLVPRVRE
                     APYEQQEEIRRRIQQRFEAARTTINAHEAAVRSLRGLAGHGWRRARP"
     gene            645..1385
                     /locus_tag="VEV43_09245"
     CDS             645..1385
                     /locus_tag="VEV43_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HYP23746.1"
                     /translation="MTLRVNCWSGPRNVSTALMRSFAQRRDTRALDEPLYGHYLLTTG
                     APHPGRDELVEILETDAATIVSETVLGPCDREVLFMKQMVHHLTPDLDLGFLDECVNV
                     QLIRDPAEVISSLVHQLPEPTMRDVGLERQRVLFDDLRARGQEPPVLDAKELLLDPEG
                     VLRELCERLGIGWDPAMLSWPAGPHPEDGVWARYWYDKLWESTGFAPYRPKDATVPES
                     CRALLEGCRPHYEELRRHAITAAPAGAG"
     gene            1386..2315
                     /locus_tag="VEV43_09250"
     CDS             1386..2315
                     /locus_tag="VEV43_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HYP23747.1"
                     /translation="MPSRRYAPEDLPDPRNADILVHVDGALVPRAEARVSVFDSVVQG
                     GDAVWEGLRVYEGRVFALDRHLDRLFASAHALRFAGLPSREEVRHAVVDTLAANGMYD
                     DAHVRLTLTRGPKVTSGMSPELNRAGCCLIVLAEWKAPVYDSTGIRLVTSSIRRNSPA
                     TLDSKIHHNNLLNNILAKIEADVAGVDDALMLDLHGFVAETNATNVFLVRGGVLVTPR
                     ADACLPGITRAIVVEVAAAAGIVTEERNVSLSEVYTAAEAFTSGTMGELAPVLEVDGR
                     VIGEGEPGPVTRRLQELHRDRTREEGEPLPPRG"
     gene            complement(2298..2612)
                     /locus_tag="VEV43_09255"
     CDS             complement(2298..2612)
                     /locus_tag="VEV43_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYP23748.1"
                     /translation="MIIRIFDTSVDPGDVEQAVAVFGTDVSPAFEAFDGCHGIELLVG
                     TDEHSGDLVELCAISRWDSRDAIDAAIGSDDYRAALAEFRKLFQQTPIVRHFEGGLPA
                     GR"
     gene            complement(2638..3474)
                     /locus_tag="VEV43_09260"
     CDS             complement(2638..3474)
                     /locus_tag="VEV43_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYP23749.1"
                     /translation="MRGHHLTIAQISDLHCGHPMFDAELLDHAIEEILELKPNLVAVV
                     GDLTSEGYGPQFRQAKRYLDRLSDLEMLVIPGNHDLMNVGFLHFRDAFGKSDRVMRLP
                     MTPPDGGEPQRWATVVAVNSSKPDLAEGEVGHIRYDWIRAGYEWPDDYRIFVLHHHLV
                     SVPGTGRERNIVWDAGDVLALLTELDVSLVLAGHKHVPNVWQFGEMLLVNSGTASSHR
                     VRGYTRPSYNVIEIDEERVRIVQRYPGMGEQVAATYHHAERRLKLNPQLEGMFNRGAW
                     NV"
     gene            3529..4206
                     /locus_tag="VEV43_09265"
     CDS             3529..4206
                     /locus_tag="VEV43_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYP23750.1"
                     /translation="MEPIYLLTKGLMKPWLLAWFRWRVEGAENIPRHGPAIVAINHIS
                     YMDPFAGCYLVDHCKRRPRFLAKSELFQDKRISWILRGCGQIEVKRGTARAPVALDHA
                     LEALKRGEVVVVFPEGTITKDADLNPMTPKTGAARLALESGAPLIPCAVWGTQNIWPK
                     GFAKNWRPRQLILIRIGAPLDFSGDPQSRDAWAEVGDKLMSAIANLLSGLREELPDRR
                     KPKKKAA"
     gene            4208..4720
                     /locus_tag="VEV43_09270"
     CDS             4208..4720
                     /locus_tag="VEV43_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYP23751.1"
                     /translation="MKVAFVGAGSWGTATAALVANKQKYDTYLWARRAELAETINLYH
                     ENPEYLPDARLPESLRATNDLEEALTGAEIVVMGVPSHGFRGVLKDVASIVGSKPCYV
                     SLTKGIEVENRKRMSEVLREEVEGIGDSCISVLTGPNLAKEVVRGYPAASTVACSDEG
                     VATRLQEIFQ"
     gene            4721..>5113
                     /locus_tag="VEV43_09275"
     CDS             4721..>5113
                     /locus_tag="VEV43_09275"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYP23752.1"
                     /translation="MTFRCYRNTDLVGCELGGALKNVIAIAAGMADGLGFGDNTKATV
                     MTRGLAEMARFGVKFGAHPLTMLGLAGVGDLMATCASPQSRNHFVGIELGKGRKIDEI
                     IGSMNMVAEGVKSCKPIFELGQAAGVWMP"
BASE COUNT          813 a         1734 c         1773 g          793 t
ORIGIN      
        1 acgggcgggc gcgacgccac ccgtgcccgg cgaggccgcg caaggatcga accgcggcct
       61 cgtgcgcgtt gatcgtcgtc ctggcggctt cgaaccgttg ctggatgcgg cgccggatct
      121 cttcctgctg ctcgtagggc gcctcgcgca cgcgggggac cagcacgcca ttgcactcct
      181 cgatcaactg ctccgttgcg tcgcggacgt ccttccacgg cacgtggagc tgcatgaccc
      241 ggtcgcgcaa ggcctcgacg tcccgttcgg tgggatattc gccggcgtcc acgtcgaggt
      301 ggtcgagagc cgccgccttg agctgcctga tccgctcgtg caggtcggag agcaagaacg
      361 ccttcaggcc gcgtctactc caccacatcg cgacccccga catcccaagg ctcacgacgg
      421 cgatccccac gcccaaccag tcctccatcg agggccgagc ctaaccagag gctcgactgc
      481 atttgaagac aagacggaca agcgttcggt tgagccagcg cccggcacct ccttccgttg
      541 ggcttccggc ccgacgctag cgaacgtgcg ttccggtcgg gcggttatcg gcgagaggct
      601 ggcgacgtgt aggcgcctcg attcgtcggt aataaggtcc cgcgatgacc ctccgggtga
      661 attgctggtc cggcccccgc aacgtctcga ccgcgctgat gcggagcttc gcccagcgcc
      721 gcgacacgcg ggcgctcgac gaaccgttgt acgggcatta cctgctcacg acgggggccc
      781 ctcaccccgg ccgggacgag ctcgtcgaga tcctcgagac cgacgcggcg acgatcgttt
      841 cggagaccgt cctcgggccc tgcgaccgcg aggtcctctt catgaagcag atggtccatc
      901 acctcacgcc ggacctcgac ctcggcttcc tcgacgaatg cgtcaacgtg cagctgatcc
      961 gggaccccgc ggaggtcatc tcgtcgctcg tccaccagct tcccgaaccg acgatgcgcg
     1021 acgtcggcct ggaacgccag cgggtcctgt tcgacgacct ccgcgcccgg gggcaggagc
     1081 ccccggtgct cgacgcgaag gagcttctgc tcgaccccga gggcgtactg cgcgagctgt
     1141 gcgaacggct cgggatcggc tgggacccgg cgatgctgtc gtggccggcc ggccctcatc
     1201 ccgaggacgg ggtgtgggcg cgctattggt acgacaagct gtgggagtcg acgggcttcg
     1261 cgccgtaccg gccgaaggac gcgaccgtcc cggagtcgtg ccgcgccctg ctcgagggat
     1321 gccgcccgca ctacgaagag ctgagacgcc acgcgatcac cgcggcgccg gcgggggcgg
     1381 gttagatgcc gagccgccgc tacgcgcccg aggacctccc cgatccccgc aacgcggaca
     1441 tcctcgtcca cgtggacgga gcgctcgtcc cgcgtgccga ggcgcgcgtg tcggtcttcg
     1501 actccgtcgt ccagggcgga gacgccgtgt gggagggcct ccgggtctac gagggcaggg
     1561 tcttcgcgct cgaccgccac ctcgaccggc tgttcgcgtc ggcgcacgcg cttcgtttcg
     1621 ccgggctgcc ctcgcgggaa gaggtccggc acgcggtcgt cgacacgctg gccgcgaacg
     1681 ggatgtacga cgacgcgcac gtgcgcctga ccttgacgcg ggggccgaag gtcacgtccg
     1741 gcatgtcgcc ggagctcaac cgcgcgggct gctgcctcat cgtgctcgcc gagtggaaag
     1801 cgcccgtgta cgacagcacg ggcatccgcc tggtcacgtc ttcgattcgg cgtaattcac
     1861 cggcgacgct cgactccaag atccaccaca acaacctgct gaacaacatc ctcgccaaga
     1921 tcgaggcgga cgtggccggg gtcgacgacg cgctgatgct cgacctccac gggttcgtcg
     1981 ccgagaccaa cgcgacgaac gtgttcctcg tgagaggagg cgtgctcgtg actccccgtg
     2041 cggacgcgtg cctgcccggg atcacgcgcg caatcgtcgt cgaggtagcg gcggccgcgg
     2101 ggatcgtcac ggaggaacgg aacgtctcgc tctccgaggt ctacacggcc gcggaggcct
     2161 tcacgagcgg gacgatgggg gagctcgcgc cggtgctcga ggtcgacggc cgggtcatag
     2221 gcgaagggga gcccgggccg gtcacgcgcc gattgcagga gcttcaccgc gaccgcaccc
     2281 gcgaggaggg agagccgcta ccgccccgcg ggtagcccgc cctcgaagtg ccgcacgatc
     2341 ggggtctgct ggaagagctt gcggaactcg gcgagggcag cgcgatagtc gtcgctcccg
     2401 atggcggcgt cgatcgcgtc gcgcgagtcc cagcgcgata tcgcgcacag ctcgaccagg
     2461 tcgcccgagt gctcgtccgt gcccacgagc agctcgatgc cgtgacagcc gtcgaacgcc
     2521 tcgaacgcgg gagagacgtc ggtcccgaag accgcgaccg cctgctcgac gtcgcccggg
     2581 tcgaccgacg tgtcgaagat gcggatgatc acgaccgcca cctttcctcg cgttcattca
     2641 tacgttccag gcccccctgt tgaacatccc ctcgagctgg gggttcagct tcagccgccg
     2701 ctcggcgtgg tggtacgtcg cggccacctg ctcgcccatc ccgggatacc gctgcacgat
     2761 ccggaccctc tcctcgtcga tctcgatcac gttgtaggag ggccgcgtgt agccgcggac
     2821 gcggtggctc gacgccgtgc ccgagttgac gagcagcatc tcgccgaact gccacacgtt
     2881 cggtacgtgc ttgtggccgg cgagcacgag gctcacgtcc agctcggtga gcagcgcgag
     2941 gacgtcaccc gcgtcccaca cgatgttgcg ttcgcggccc gtcccgggga ccgagaccag
     3001 gtggtggtgg aggacgaaga tccggtaatc gtcgggccac tcgtagccgg cgcggatcca
     3061 gtcgtagcgg atgtgaccga cctctccctc ggcgaggtcg ggtttcgagg agttgaccgc
     3121 tacgacggtc gcccaccgtt gcggctcccc tccgtccggc ggggtcatcg gcaggcgcat
     3181 gacgcggtcg ctcttgccga acgcgtcgcg gaagtgcagg aagccgacgt tcatgaggtc
     3241 gtggttcccg gggatgacga gcatctccag atccgagagc cggtcgaggt agcgcttcgc
     3301 ctgacggaac tgcgggccgt agccctccga ggtcaggtcg ccgaccaccg cgacgaggtt
     3361 cggcttcagc tcgaggatct cctcgatggc gtggtcgagg agctccgcgt cgaacatggg
     3421 gtggccacag tgcaggtcgg agatctgagc gatcgtcaga tggtggcctc tcacggggtc
     3481 cgtatgatag gtcgagctgt ccgagcgcgg cactattacg ggggattctt ggagccgatc
     3541 tacctgctga cgaaggggct catgaagccg tggctcctcg cgtggttccg ctggcgcgtc
     3601 gagggggccg agaacatccc gcggcacggc cccgccatcg tcgcgatcaa ccacatctcg
     3661 tacatggacc cgttcgcggg ctgctacctc gtcgaccact gcaagcggcg cccccggttc
     3721 ctcgccaagt ccgagctctt ccaggacaag cgcatctcct ggatcctgcg cggctgcggc
     3781 cagatcgagg tcaaacgagg gacggcgcgc gctcccgtgg cgctcgacca cgcgctcgag
     3841 gctctcaaga ggggcgaggt cgtcgtcgtc ttcccggagg ggacgatcac gaaggacgcc
     3901 gacctgaacc cgatgacgcc gaagaccggt gccgccaggc tcgcgctcga gtccggagca
     3961 ccgctgatcc cgtgcgccgt gtgggggaca cagaacatct ggcccaaggg cttcgcgaag
     4021 aactggcggc cgcgccagct gatcctgatc cggatcggcg ctccgctcga cttctccggc
     4081 gacccgcagt cgcgcgacgc gtgggccgag gtcggcgaca agctgatgag cgcgatcgcg
     4141 aacctgctct cggggctgcg cgaggagctg cccgaccgcc gcaagcccaa gaagaaagcc
     4201 gcctgagatg aaggtcgcct tcgtaggcgc cggctcgtgg ggcacggcga ccgcggccct
     4261 cgtcgcgaac aagcagaagt acgacaccta cctgtgggcg cgccgggccg agctggccga
     4321 gacgatcaac ctgtaccacg agaaccccga gtacctgccc gacgcgcgcc tgcccgagag
     4381 cctccgcgcg acgaacgacc tcgaagaggc cctgaccggc gcggagatcg tcgtgatggg
     4441 cgtgccttcg cacggtttcc gcggcgtgct caaggacgtc gcttcgatcg tggggtcgaa
     4501 gccgtgctac gtgagcctga cgaagggcat cgaggtcgag aaccgcaagc gcatgtccga
     4561 ggtgctgcgg gaagaggtcg agggcatcgg ggactcgtgc atctcggtcc tgaccgggcc
     4621 gaacctcgcg aaggaggtcg tgcgcggcta tcccgcggcc tcgacggtcg cgtgcagcga
     4681 cgaaggtgtc gcgacccggc tgcaggagat cttccagtag ctgacgttcc gctgctaccg
     4741 caacaccgac ctcgtcggat gcgagctcgg cggggcgctg aagaacgtga tcgcgatcgc
     4801 cgcgggcatg gcggacgggc tcggcttcgg cgacaacacg aaggccacgg tgatgacgcg
     4861 ggggctcgcc gagatggcgc gcttcggggt gaagttcggg gcccacccgt tgacgatgct
     4921 cgggctggcg ggcgtggggg acctcatggc gacgtgcgcg agcccgcagt ctcgcaacca
     4981 cttcgtcggg atcgagctcg gcaagggccg caagatcgac gagatcatcg gctcgatgaa
     5041 catggtggcc gaaggtgtga agagctgtaa gccgatcttc gagctgggcc aggccgccgg
     5101 cgtgtggatg ccg
//
LOCUS       DASQDO010000330         8906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_341149, whole genome shotgun sequence.
ACCESSION   DASQDO010000330 DASQDO010000000
VERSION     DASQDO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341149"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VEV43_09280"
     CDS             complement(<1..644)
                     /locus_tag="VEV43_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HYP23753.1"
                     /translation="MAKESRLPKQEDDFPAWYQDVVKQSGMAEHGMARGSQVIKPHGY
                     AVWENIQRALDDRFKATGHENLMFPLLFPLSLLEKEAEHVEGFAPELAVVTIGGGEEL
                     EEPLVIRPTSEAVIWTTYGNWIQSYRDLPLLYNQWCNVLRWEKRTRLFLRTSEFWWQE
                     GHTAHATTQEAWEETVRMLEVYREVATDVLAIPVLAGRKSESERFPGADETLPI"
     gene            complement(647..1006)
                     /locus_tag="VEV43_09285"
     CDS             complement(647..1006)
                     /locus_tag="VEV43_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HYP23754.1"
                     /translation="MKTHQIECTATTKTALDFVDITDEVQGALAASGIRSGQVTVFAP
                     HRDCAILINERESGLHTDIRTTLERLGGKRTVVGSKSVVLPAMNGELRLGQWQRVLLL
                     ELDRAQERSIIVQIVGE"
     gene            1618..1989
                     /locus_tag="VEV43_09290"
     CDS             1618..1989
                     /locus_tag="VEV43_09290"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HYP23755.1"
                     /translation="MFADEEEIENEEELEDDELESDDEDDEDLALAAGDDEDEEDESS
                     LEELLAQRAAARRGTDDSDDDEDLLSLSSEPETIPPIEPIPARVVPLKDRQEFVCNRC
                     HLVKARVQLADAERGLCRDCV"
     gene            2012..2770
                     /locus_tag="VEV43_09295"
     CDS             2012..2770
                     /locus_tag="VEV43_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HYP23756.1"
                     /translation="MARALVIVPTYDERENVAEVARRLFDAAGDSVDLLVVDDGSPDG
                     TAALVKTLAAESSHGIHVIERSHKQGLGTAYVTGFKWGLERGYWALVEMDADLSHDPA
                     DVPRLLARLEAGADLAIGSRYVDGGGTENWGPLRRALSSGGNLYARMWLGYPVRDSTA
                     GFRAYRAEVLAREDLSTVRSEGYGFQIEMTRRVWKAGGTIAEVPITFTERVQGKSKMS
                     RRIVVEALAQVTRWGLRDLGDRRRVRRGGGGASP"
     gene            complement(2739..4658)
                     /locus_tag="VEV43_09300"
     CDS             complement(2739..4658)
                     /locus_tag="VEV43_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23757.1"
                     /translation="MWWTRTFGRGRAAAFAVALLLPAGVVVTAAAQSAGPMQITLVPV
                     QSSPRAPGCSELTARLTDANGDPVQGAVLDVRQTLRDPDEATIGFCNHPDPRGPNPTG
                     HGGTAFGDVSGDARSVHAEVGPTDVDGHVSFGVSTSSHVPATVDVLVWLDLSGDDDVF
                     QSHEPSRSAEVHWWPGEMWFNPSIDATPEAASVQNGTQHTVTVRIMAGQSPITGVVPT
                     SVIVASAAGGSGDVAASAAGGSGDVADPNAGPSPNYAPGTTTANAYSCTASNGQGFST
                     CTFQDPLSTGPGTDTVVFSFEDPRAPESAPEPRDTVQVTWTAPGSTPPPTPTPSSTPT
                     ATPTASPAPTASPSPAPGPEPRNVRLCHEDAGAGVACDASFRATEAGAELRVAVFVTD
                     RNGAPAQNVPVELRESGPAEFVATGASTILVYTGSDGVARATLVAEEGGDSTVVAEIS
                     PPGIPGSVRGPGASDDACEQPGGNCVSQTLTVHWEEEVPHACNDGTDNDGDGYVDHPE
                     DPGCSEPDDDSETPAPHPSAERHSRAAGLRFAHDDETGRLLVYGKVRLTDAGDDYEEC
                     MKRRPVEVQRRVDGDWETLKTPETDDRGRYTASVPDRPGRYRATALRTSHIDDAGLVH
                     VCKRAVRAKRHLHRA"
     gene            complement(4898..7045)
                     /locus_tag="VEV43_09305"
     CDS             complement(4898..7045)
                     /locus_tag="VEV43_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HYP23758.1"
                     /translation="MRFRSAAAAIAAGLIATTIVPAAAMATPVCTDGYQGGPPQAVCG
                     GRIFPEAAVARSYVQYLPDPATGFREYEHGVEYLAEAYPRWVSVFKLSDIYEKDAVSA
                     WEDGIRSYEKDDTEDGLEILVIKLTDHDVPDRGKETLFFSLSVHGNERGGLEGGLRAV
                     EDLARAAEDGGTIVDGIDNYETTTGRTPKFHEYDVKDVLRREAVYLVDFNPDGWAVGD
                     HYHDPPLPYERENQMGTDLNRQMPTVGRINEGRNPLEESEMFYGHKFMHQVASEGING
                     KMAYGADVHGELTSNAYVDIMYPAGQFDSVDHRRLMAIAERTKSVIDATLYQGIVDQI
                     EEGSGGNSSNPDSDVPTKPAAWGTVWDTLGYTDTGFIGDYLATDLGVTGMDYEIFLNH
                     TVPDKNWNVYLQENHINATRAIIKTAMAYALTQSKEFNARNVRLDPKGTAGYVFDPKP
                     VSSRDKNGPGTLAGPNKNGRGANGSKVRQRHYRVSNMKWFKDTSRLMSKPFVPMNTRK
                     IARQRGYLDKLDTLVLADRARPRDTKGRVASPKTYFANIAKWVRKGGNLVLTDRALRV
                     LHKIGVVGKDAVSNVKVYQPYADFVDFEHPMVAGLRPNARQLVEAPILGYCIGGDCSP
                     MTVVDETAFTDAGGHVIGTTGDGRVSVGELELGKGTIRILGGALPRPTEAFDHRYGLR
                     DYALTYSGLYIMENSMVHDAPGLGASKKLTRTL"
     gene            complement(7130..7951)
                     /locus_tag="VEV43_09310"
     CDS             complement(7130..7951)
                     /locus_tag="VEV43_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23759.1"
                     /translation="MIREQALEKGDPSASSSSGAGSASSAWMQQVKTQIKALKSFLQE
                     DNTYDAHIRDKKGWEDTAGIRWDRGSASEIVATLEKLDRELRKVPDEGRDRLETQITK
                     TLSVFLSARPYIMEYYAEDRREKWHAAAASPPVVAQPNARQAPVATANERARRRAGEG
                     HPGTAYCAAGYGDSFHSGSTGHGLRLNDLHATLSHILQSSEQLEKWGNKACAEIPAIN
                     RALNAGLDAKKLRISTARQDDRGSVSNVPPCENCRAWLELIGGTHDYRIRPSKLP"
     gene            complement(8074..>8906)
                     /locus_tag="VEV43_09315"
     CDS             complement(8074..>8906)
                     /locus_tag="VEV43_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23760.1"
                     /translation="LVDEFFQSDGKPYGHGLARAPEDPMHDSRAGYYSFPMGPKVRGI
                     SLDTISYDGVSEGHIPDPQFKWLRRELRKWSKTYYAGGERRRSRKGRDRLIVVFSHHS
                     SETLRNPGGDAEGHPFHCFRKTDQPECEAGEGLKNLLHRFPNVVAWVNGHEHNNAVRA
                     FPAPGKKDPARGFWEVNTAAHIDWPQQSRLIEIAWKPGRRADTVLIYGTVVDHGAAPD
                     PDRGGQSTTAYLSSVSRVEAFYDACTREGQADCAAPGRRKDRNVRLLIKAPFDLGRSS
                     "
BASE COUNT         1235 a         2993 c         3139 g         1539 t
ORIGIN      
        1 tcgatcggga gcgtctcgtc ggcgcccggg aaacgctcgg actcggactt gcgtccggcg
       61 agcacgggga tcgccagcac gtcagtcgcc acctcgcggt agacctcgag catgcggacg
      121 gtctcctccc acgcctcctg cgtggtggcg tgcgcggtgt ggccctcctg ccaccagaac
      181 tcgctcgtgc gcaggaacag ccgcgtccgc ttctcccagc gcagcacgtt gcaccactgg
      241 ttgtagagga gcgggagatc gcggtagctc tggatccagt tcccgtacgt cgtccagatc
      301 acggcctcgg acgtgggccg gatgaccagc ggctcctcca gctcctcgcc gccaccgatc
      361 gtcacgacgg cgagctcggg agcgaaccct tcgacgtgct cggcctcctt ctcgagcagg
      421 ctcagcggga acagcagcgg gaacatgagg ttctcgtgcc cggtcgcctt gaagcggtcg
      481 tcgagcgcgc gctggatgtt ctcccacacc gcgtagccgt ggggcttgat tacctgggag
      541 ccgcgcgcca tcccgtgctc ggccatgccc gactgcttga cgacgtcctg gtaccaggcg
      601 gggaagtcgt cttcctgctt ggggagcctg ctctctttcg ccatgtctat tcgcccacta
      661 tctgcacgat gatcgacctt tcctgtgccc ggtccagctc gaggagaagg accctctgcc
      721 attggccgag ccggagctcg ccgttcatgg ccgggagcac gacggacttc gacccgacga
      781 cggtccgctt gccgcccagc ctctccagcg tcgtccgtat gtcggtgtgc aaccccgact
      841 cgcgctcgtt gatgagtatg gcgcagtcac gatgaggcgc gaagaccgtg acctgtccgg
      901 agcggatgcc cgacgcggcg agcgccccct gtacctcgtc ggtgatgtct acgaaatcga
      961 gcgcggtctt cgtcgtcgcc gtgcactcga tctggtgcgt cttcatgacc tggcatggtc
     1021 gccgcaatcg ccgcattcgg caaaccgcca caacttgtgc agaacctgtg ggtcgagggg
     1081 gtggaaacct gcggagaaca cgaatgtttc gcggcctcgg aagttctcgt cgacgcgcta
     1141 ttgaccgggc cttcgagccc gcctatgttg cgacctgtcc caagcaatca aacggcacag
     1201 ccgccgccgg cgccaaccga aagggaggcg ccgacccggc cggaggaaag ctcctcggag
     1261 cggaccgccg gcgggggagc gggggaccgg ttcgccggtc gcccgcgacg gtgacgggcc
     1321 gggcggttgc ttgggacatt gagattggcc ccaaagccac ttgggttcgc cctagtcgct
     1381 ttgggacccg ggtgagaggc ccgagtggct cgaggacaac gctccaataa ccggtcacgg
     1441 ccggctaacg agcggcccct ccggtccctc gggcctttct ctttctcact tgtgcaggag
     1501 tcctgccgtc cccgcaaaac cttcgcgctt ggcgctgcgt cctaggggaa tagaactcgc
     1561 cggacggggt tccgtagggt ttggccgttg acggggattg ccgaaggagt tgcgcgggtg
     1621 ttcgccgacg aggaagagat agagaacgaa gaagagctcg aagacgacga gctcgaaagc
     1681 gacgacgagg acgacgagga ccttgccctc gccgccggtg acgacgaaga cgaagaggac
     1741 gagtccagcc tcgaggagct gctcgcgcag agggccgcgg cgcggcgggg gaccgacgac
     1801 tcggacgacg acgaggacct gctgagcctc tcgtcggagc ccgagacgat cccgccgatc
     1861 gagccgatcc cggcgcgggt ggtcccgctc aaggaccgcc aggagttcgt gtgcaaccgc
     1921 tgtcacctcg tgaaggcgag ggtccagctg gcggacgccg agcgcggtct ctgtcgcgac
     1981 tgcgtctagc gcagttcgtt acggtgccgt cgtggcccgt gccctcgtaa tcgtcccgac
     2041 ctacgacgag cgcgagaacg tcgcggaggt cgcgcggcgt ctgttcgacg cggcgggcga
     2101 ctcggtcgac ctgctcgtcg tcgacgacgg gtcgccggac gggacggccg cgctggtgaa
     2161 gacgctggca gccgagtcgt cgcacgggat ccacgtgatc gagcggtcgc acaagcaggg
     2221 cctcgggacg gcctacgtga ccggtttcaa gtgggggctc gagcgcggct actgggctct
     2281 ggtcgagatg gacgcggacc tgtcgcacga tcccgccgac gttcctcgtc tgctggcgcg
     2341 actggaggcc ggcgcggacc tcgcgatcgg ctcccgctac gtcgacggag gcgggaccga
     2401 gaactggggg ccgctgcgcc gggccctctc gtcgggcggg aacctgtacg cccggatgtg
     2461 gctcggctac ccggtccggg actcgacggc cggcttccgt gcgtaccggg ccgaggtctt
     2521 ggcgcgcgag gacctgtcga ccgtgaggtc ggagggttac ggcttccaga tcgagatgac
     2581 gcgccgggta tggaaggccg ggggcacgat cgccgaggtc ccgatcacgt ttacggagcg
     2641 ggtccagggc aagtcgaaga tgagccgccg gatagtcgtg gaggcactag cccaggtgac
     2701 ccgctggggg ctgcgggacc tcggcgatcg acgccgcgtc aggcgcggtg gaggtggcgc
     2761 ttcgccctga ccgccctctt gcagacgtgc acgagtccgg cgtcgtcgat gtgcgaggtg
     2821 cgtagagcgg tggcgcggta gcggccgggt cgatcgggga ccgatgccgt gtatctcccg
     2881 cggtcgtccg tctcgggcgt cttcagcgtt tcccagtcgc cgtcgacgcg gcgttggacc
     2941 tccaccggcc ggcgcttcat gcactcctcg tagtcgtcgc cggcgtctgt gaggcggacc
     3001 ttgccgtaga cgagcaagcg acctgtctcg tcgtcgtggg cgaagcgcaa tccggcggcc
     3061 cgcgagtgtc gctccgccga ggggtgaggc gccggtgtct cggagtcgtc gtccggctcc
     3121 gagcatccgg ggtcctcggg gtggtcgacg tacccgtcgc cgtcgttgtc ggtcccgtcg
     3181 ttgcaagcgt gcggaacctc ctcctcccag tggacggtca acgtctgcga gacgcagttc
     3241 ccgccgggct gctcgcaggc gtcgtccgac gcacccgggc cgcggacgct gccgggtatt
     3301 cccggggggc tgatctcggc gacgacggtc gagtccccgc cttcctcggc tacgagcgtc
     3361 gcgcgcgcca cgccgtcgct ccccgtgtag acgaggatcg tcgacgcacc ggtggcgacg
     3421 aactcggccg ggccgctctc gcggagctcg accgggacgt tctgcgcggg cgctccgttg
     3481 cggtcggtga cgaagactgc gacgcgcagc tcggcgcccg cttcggtggc ccggaacgac
     3541 gcgtcgcaag cgacgccggc gccggcatcc tcgtggcaaa ggcggacgtt gcgcggctcc
     3601 ggtccggggg ccggggacgg ggaagcagtg ggggccggtg aagcagtggg ggtcgctgtc
     3661 ggggtcgagg atggggtcgg ggtcggcggg ggagtcgacc cgggcgccgt ccacgtgacc
     3721 tgcaccgtgt cccgcggctc cggcgcggac tccggtgcgc gagggtcttc gaaggagaag
     3781 acgaccgtgt ccgtcccggg cccggtggac agggggtcct ggaacgtgca ggtcgagaat
     3841 ccctgcccgt tgctggcggt gcacgagtac gcgttcgcgg tggtggtccc cggggcgtag
     3901 ttggggctcg gcccggcgtt cgggtccgcg acgtcaccgg agccgccggc ggcgctggcg
     3961 gcgacgtcac cggagccgcc ggcggcgctg gcgacgatca cgctggtcgg gacgacgccc
     4021 gtgatcgggc tctggccggc cattatccgc accgtgacgg tgtgctgcgt cccgttctgg
     4081 acggaggcgg cctccggggt tgcgtcgatg ctcgggttga accacatctc gccgggccac
     4141 cagtgcacct cggcggagcg tgagggctcg tgcgactgga acacgtcgtc gtcgccgctc
     4201 aggtcgagcc atacgagcac gtccacggtg gcgggcacgt gcgacgacgt cgacacaccg
     4261 aagctgacgt gcccgtcgac gtccgtcggg cccacctcgg cgtgtaccga tcgggcgtcg
     4321 cccgacacgt cgccgaaagc ggtcccgccg tggcccgtcg ggttcggccc ccgggggtcg
     4381 ggatggttgc agaagccgat agtcgcttcg tcgggatccc gcagcgtctg acggacgtcg
     4441 aggaccgctc cttgaacggg gtctccgttg gcgtcggtca ggcgtgcggt gagctcgctg
     4501 cagccgggcg cccggggcga gctctggacc ggcacgagcg tgatctgcat gggcccggcg
     4561 ctctgcgcgg ccgcggtgac gacgacgccg gccggcagga gcagggcgac ggcgaacgca
     4621 gcggcccggc ctcgcccaaa cgtcctcgtc caccacatag cgacaccccc tcgtccgtcc
     4681 ctacatgatg atgcaacgac cccgttccgg acggccgcca ggccgccgct tgttggccaa
     4741 agcgagacgg cccggctcgg cgcccataat gcacgctatg taaacctcac gaggtggagc
     4801 gagcgggagg ggtcccccgc ggcgggcccg gcccccgccg cgtgcgcagg cagtggcgga
     4861 aaccgcaacc ggctgcgcac gcggggcagg ctctcggcta cagcgtgcgg gtcagtttct
     4921 tcgaggctcc caggcccggg gcgtcgtgga ccatcgagtt ctccatgatg tacagacccg
     4981 agtacgtcag cgcgtagtcc ctcagcccgt agcggtggtc gaaggcctcg gtcggcctcg
     5041 gcagggcccc gcccaggatc cggatcgtgc ccttgcccag ctcgagctcg cccacgctca
     5101 cccggccgtc gccggtcgtg ccgatcacgt ggcccccggc gtccgtgaag gcggtctcgt
     5161 cgacgaccgt catcggcgag cagtcaccgc cgatgcagta gccgaggatc ggcgcctcga
     5221 cgagctgacg ggcgttcggg cggagcccgg cgaccatcgg gtgctcgaag tccacgaagt
     5281 ccgcatacgg ctggtagacc ttgacgttgc tgacggcatc cttgccgacg acgccgatct
     5341 tgtggagcac ccgcagggcc cggtcggtca agacgaggtt cccgcccttg cggacccact
     5401 tcgcgatgtt ggcgaagtac gtcttcgggc tggcgaccct ccccttcgtg tcgcggggcc
     5461 gagcccggtc ggcgagcacg agcgtgtcga gcttgtcgag gtagccgcgc tggcgcgcga
     5521 tcttgcgcgt gttcatcggg acgaacggct tggacatcag acgagacgtg tccttgaacc
     5581 acttcatgtt gctcacgcgg tagtgacgct ggcggacctt gctcccgttc gcgccgcgcc
     5641 cgttcttgtt cggccccgcc agcgtccccg gaccgttctt gtcacgcgac gacacgggct
     5701 tcgggtcgaa gacgtagccg gcggtcccct tcgggtcgag ccggacgttg cgcgcgttga
     5761 actccttgct ctgcgtaagc gcgtacgcca tcgccgtctt gatgatggcg cgcgtggcgt
     5821 tgatgtggtt ctcctggagg tagacgttcc agttcttgtc cggcacggtg tggttcagga
     5881 agatctcgta gtccatgccg gtgacgccga ggtccgtggc gaggtagtcg ccgatgaacc
     5941 cggtgtcggt gtagccgagc gtgtcccaga cggtccccca cgctgcgggc ttggtcggga
     6001 cgtccgagtc ggggttgctg ctgttgccgc cgctaccctc ctcgatctgg tcgacgatcc
     6061 cctggtacag cgtcgcgtcg atgacggact tcgtccgctc agcgatcgcc atgaggcgcc
     6121 ggtggtcgac cgagtcaaac tggccggccg ggtacatgat gtcgacgtag gcattcgagg
     6181 tgagctcgcc gtgcacgtcg gcgccgtagg ccatcttgcc gttgatgccc tccgacgcca
     6241 cctggtgcat gaacttgtgg ccgtagaaca tctcgctctc ctcgagcggg ttccggccct
     6301 cgttgatgcg cccgaccgtc ggcatctggc ggttgaggtc ggtgcccatc tggttctcac
     6361 gctcgtacgg gagcggcggg tcgtggtagt ggtcccccac cgcccagccg tcggggttga
     6421 agtcgacgag gtagacggcc tcgcgccgca gcacgtcctt gacgtcgtat tcgtggaact
     6481 tcggagtgcg gcccgtggtc gtctcgtagt tgtcgatccc gtcgacgatc gtcccgccgt
     6541 cctcggcggc gcgggcgagg tcctcgacgg cgcgcaggcc gccctcgagc cctcccctct
     6601 cgttaccgtg gaccgacagc gagaagaaca gcgtctcctt gccgcggtcg ggaacgtcgt
     6661 ggtcggtgag cttgatgacg aggatctcga gcccgtcctc ggtgtcgtcc ttctcgtacg
     6721 accggatgcc gtcctcccag gcgctcacgg cgtccttctc gtagatgtcc gagagcttga
     6781 agaccgagac ccagcgcggg tacgcctcgg cgaggtactc gacgccgtgc tcgtactcgc
     6841 ggaagcccgt ggccgggtcc ggcaggtact ggacgtacga ccgcgcgacg gcggcctcgg
     6901 ggaagatgcg cccgccgcag acggcctgtg gaggcccgcc ctggtagccg tcggtgcaga
     6961 ccggcgtggc catggcggcc gccgggacga tggtcgttgc aatgagcccg gccgctatgg
     7021 cggccgctgc cgatcggaac ctcatgaagc ctcccgcgag aagactgacc ctgttctgtc
     7081 agggttcgcc ccttttgcgg cgttacctgc gggggccggg agccgggccc tagggcaact
     7141 tactcggcct gatccggtaa tcgtgcgtcc cgccgatcaa ctcgagccac gcccggcagt
     7201 tctcgcacgg cgggacattg ctgacactcc ctcggtcgtc ttgcctggcc gtagagatcc
     7261 gcagcttctt ggcgtccagc cccgcgttca aagcgcggtt gatcgcagga atctccgcgc
     7321 aggccttgtt cccccacttc tcgagctgct ccgagctctg gaggatatgg ctgagcgtgg
     7381 cgtggagatc gttgagcctg aggccgtgtc cggtacttcc cgagtggaag ctgtcgccgt
     7441 agcccgccgc gcagtacgcc gtaccgggat gaccctcccc ggcacgtcga cgggcccgtt
     7501 cgttcgccgt ggcgacgggg gcctgccggg cattcggctg cgctaccacc ggaggtgagg
     7561 cggcggctgc gtgccacttc tctctccggt cctcggcgta gtactccatg atgtacggac
     7621 gcgccgagag gaagacgctc agcgtcttcg tgatctgggt ctccaaccgg tcgcgtccct
     7681 cgtccggcac cttccggagc tcccgatcca gtttctcgag cgtcgcgacg atctcggatg
     7741 ccgagcctcg gtcccatcgg atgccggccg tgtcttccca gccttttttg tcccgaatgt
     7801 gcgcgtcgta ggtgttgtcc tcctgcagga agctcttcaa cgctttgatc tgcgtcttga
     7861 cctgctgcat ccacgcgctg gacgcggacc cggcgccgga cgaagaggag gcggaaggat
     7921 cgcccttctc caaggcctgc tcgcggatca tgacggtcgc cccccaggtc gtgtctcctg
     7981 acccatcctc gccccggctt ccgttggacg ccgcggccga aggtgcagac ttcggggcag
     8041 cgactcgggc cgaaaggtcc ctacgggcgt cgtctaggac gagcgtccca ggtcgaacgg
     8101 ggccttgatc aagaggcgga cattgcggtc cttgcggcgg ccgggggccg cgcagtccgc
     8161 ctggccctcg cgggtgcagg cgtcgtagaa cgcctcgacc ctcgataccg acgagaggta
     8221 cgcagtcgtc gactgcccgc cccggtccgg gtcgggcgcg gccccgtggt cgacgaccgt
     8281 cccgtagatc agcaccgtgt ccgcgcgccg gccgggcttc cacgcgatct cgatcagccg
     8341 cgactgctgc ggccagtcga tgtgcgccgc ggtgttgacc tcccagaacc cccttgccgg
     8401 gtccttcttg ccgggggccg ggaaggcgcg caccgcgttg ttgtgctcgt gcccgttgac
     8461 ccaggccacg acgttcggga agcggtggag caggttcttc aacccctcgc cggcctcgca
     8521 ctcgggctgg tcggtcttac ggaagcagtg gaacgggtgg ccctccgcgt cgccaccggg
     8581 gttcctcagc gtctcggagg aatggtgcga gaagacgacg atcagccggt ccctcccctt
     8641 ccggctccgc ctgcgctcgc cgccggcgta atacgtcttg ctccacttgc ggagctcgcg
     8701 ccgcagccac ttgaactgcg gatccgggat gtgcccctcg gacacgccgt cgtaggagat
     8761 cgtgtcgagc gagatccccc gcaccttcgg tcccatcggg aagctgtagt agccggcgcg
     8821 ggagtcgtgc atcgggtcct cgggcgcccg ggcgaggccg tggccgtacg gcttcccgtc
     8881 gctctggaag aactcgtcga cgagct
//
LOCUS       DASQDO010000331         5918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_97051, whole genome shotgun sequence.
ACCESSION   DASQDO010000331 DASQDO010000000
VERSION     DASQDO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97051"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VEV43_09320"
     CDS             complement(<1..878)
                     /locus_tag="VEV43_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYP23761.1"
                     /translation="MTAAGVSILVVDDDEALRLATARILEREGHDVTTASSVAEARAL
                     LDAYRFPIVLLDVSMPGESGLVLLEELSAAGPDTACIMVTGADSRELGERALGGGAYG
                     YVIKPFTPNEILITVSNGVRRRRLELDQRDQQERLEAAVVVRTRELWSSTLELERREE
                     QLRLANEETVRRLALAAELRDADTARHVARVGEYAGVIGRRIGFDADEIRKLELAAAL
                     HDVGKIGVPDEILRKRGRLSPEERQTMQTHTTIGHMMLSDTSIDLLDLAASVALTHHE
                     RWDGSGYPSGLAGEDIP"
     gene            complement(875..4369)
                     /locus_tag="VEV43_09325"
     CDS             complement(875..4369)
                     /locus_tag="VEV43_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYP23762.1"
                     /translation="MSIPAPIRVLLVEDNPFDAALVERELVAGGLSVHVTRAVTRDDF
                     TAGLEDADVVLVDYNLPSFDPYTAMEIAESRGHAVPFIVVTGNVDEEIAVECIKRGAG
                     DYVVKDRMARLPRAVLGVLEQEDLRRRQRAMEQSLRESEERFRSLVQNSRDVITVIDE
                     TGTCTYISPSVKALSGHDPEDVVGRSGFDFVHPEDRAGLVTHLADVVADPARTKTVEV
                     RAATVTGGWIWIEARAANRLDDPAIRGIILNYHDVSERKESERVLRESRRLLADAEAV
                     AHVGSFAWDVTGDAIVWSAEMFRIFGHEPGDVDLDYQSFLRRVHDEDRPVVAALVQDS
                     LVSGTAFEGEYRIVRADACVRWIHVRGVPGVTDAGPRIVGTAQDVTERRAADEERTRL
                     YRRQEALAEQLSLLLESTGEGIFGVDAEGRCTFVNGAALRMLGHDREEVMGTAMDELV
                     HHTAAAPGPEGASALARAFRSGQASSRDDEVYRRRDGSSFPVEFSAHPIMREGRPSGA
                     VVAFKDATHRQAMERELREREALFRGAFDAATTGIALSDAVTNAYVDVNEALCEIVGY
                     PKDELLRLDWVTLTHPDDRARSIEDVENFKSGREQVTRVSKRYVRRDGRVIQVEINDA
                     LIRDERGAPLYFVSHVSDVTEREHAAKALRESQALLQEVIDNSPAAIYIKDTDGRYVL
                     TNSRFAQIIGAHGDDLRGKTDHDLLPEDVAGTLSAMDREVLARGEPVEVEEIVPDATG
                     RRGTYLSVKFPLFDEDGTPDALVAISTDISDRVRAEEEKAMLEAQLRQAQKMEAVGRL
                     AGGVAHDFNNILAVILNYGDFVLEALPSDDASRADVEQIVDAGRRAAKLVHQLLAFSR
                     HDAVEHGPVGLNEVVEGVREILARAIGEHISLEVDCAPGLWDVKADAGQLEQVLLNLA
                     VNARDAMPDGGILTVATSSETLHERGGRGIAPGKYVKLTVADTGLGIDSATRERIFEP
                     FFTTKPRGEGTGLGLSTVYGIVAQAHGQVFVESEPGAGTSFEVYLPATDERAPEEAPS
                     PDKTSPAEVSGKILVVEDEDAVRELVARILRRSGFEVVAMPSAPVALDYCKSNLDDVD
                     LLLTDVVMPGMSGTTLAERVAALGTGVKTLYMSGYTGDAIPGRDWQGIEGNVIGKPFT
                     PEQLVSCVRAALACGAAS"
     gene            complement(4366..4824)
                     /locus_tag="VEV43_09330"
     CDS             complement(4366..4824)
                     /locus_tag="VEV43_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP23763.1"
                     /translation="MNGVQILLVEDNPSDLALALHAFKKHNLTNGVHVARDGEQALEY
                     LFKSGEHAGRESATDPQVILLDLKLPKVEGLEVLRAIKADERTRDIPVVVLTSSAAER
                     DIVETYSLGVNSYIVKPVDFDQFSEAVRVIGFYWLLLNQSPPRGQRAVAP"
     gene            complement(4821..>5918)
                     /locus_tag="VEV43_09335"
     CDS             complement(4821..>5918)
                     /locus_tag="VEV43_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23764.1"
                     /translation="VEWSFDALVGVDLDGTITSWNAAAETLYGRRRDEAVGDALATVV
                     TDEAELQRVLERALAGDVTRYETRHRTSGGAERQVWVTVSPIRDPAGEVVGSSHIVMD
                     ISDRKRAEAEAEALRRDLEERVVQRTLELQEKTRELEESMAELDSFAYSVSHDLRAPL
                     RALDGFSRILLEELGDSLSDDHAQYLSYIRANAQGMQQLVDGLLAFSRLGRSGLDLAP
                     VSPEVLVRQALSDLGAAVRPAVEISTGHLPGCVADATLLKQVFANLIDNALKFTRGVP
                     HPAVEIGSYRDGGEDVYFVRDNGVGFDPKYAEKIFGVFQRLHEPERFEGTGIGLANVQ
                     RIVEKHEGRIWCNGEPGRGAIFCFTIGTKQT"
BASE COUNT          822 a         2118 c         1999 g          979 t
ORIGIN      
        1 gggatgtcct caccggccag gccgctgggg tagccgcttc cgtcccacct ctcgtgatgc
       61 gtcagggcga cgctggccgc gaggtccaag aggtcgatcg acgtgtccga cagcatcatg
      121 tggccgatcg tcgtatgcgt ctgcatcgtc tggcgttcct cgggcgagag ccggccgcgc
      181 ttgcgcagga tttcgtccgg cacgccgatc ttcccgacgt cgtgcagcgc cgccgcgagc
      241 tcgagcttcc ggatctcgtc cgcgtcgaag ccgatcctgc gcccgatgac gccggcgtac
      301 tccccgacgc gcgcgacgtg ccgggcggtg tcggcatccc ggagctccgc ggcgagcgcg
      361 agccgccgca ccgtctcctc gttcgcgagc ctcagctgct cctcgcgccg ctccagctcc
      421 agcgtcgagc tccacagctc tcgggtgcgg acgacgacgg cggcctcgag cctctcctgc
      481 tgatcgcgct ggtcgagctc gagccgccgg cggcggacgc cgttcgagac ggtgatcagg
      541 atctcgttcg gagtgaacgg cttgatgacg tagccgtagg cgccgccccc gagggcccgt
      601 tcgccgagct cgcggctgtc ggcgcccgtt accatgatgc aggcggtgtc gggccctgca
      661 gcggacagct cctcgagcag cacgagcccg gactcgcccg gcatcgagac gtcgagcagg
      721 acgatcggga acctgtaggc gtcgagcaga gcgcgtgcct ccgcgacgct cgacgcggtc
      781 gtgacgtcgt ggccttccct ctcgaggatc cgcgccgtgg cgaggcggag cgcctcgtcg
      841 tcgtccacga cgaggatcga gacgccggcg gcggtcacga agcggctcca cacgccagcg
      901 ccgcccgcac gcacgatacg agttgctccg gcgtgaacgg cttcccgatg acgttgcctt
      961 cgatgccctg ccagtcgcga cccgggatgg catcgccggt gtagccggac atgtagagcg
     1021 tcttcacgcc ggtgccgagg gccgcgaccc gctcggcgag cgtggttccc gacatccccg
     1081 gcatgactac gtcggtcagg aggaggtcga cgtcgtcgag gttcgacttg cagtagtcga
     1141 gcgcgacggg ggccgacggc atcgcgacga cttcgaagcc gctacgcctc aggattcgag
     1201 cgacgagctc cctgacggcg tcctcgtcct cgacgacgag gatcttcccc gagacctccg
     1261 ccggcgacgt cttgtcgggg gatggggcct cctcgggagc gcgctcgtcg gtcgccggga
     1321 ggtagacctc gaacgaggtc ccggcgccgg gctccgattc gacgaagacc tggccgtgcg
     1381 cctgggcgac gatcccgtag acggtcgaca ggccgaggcc cgtcccctcc ccgcggggct
     1441 tcgtcgtgaa gaacggctcg aatatccgct cccgggtcgc cgagtcgatc ccgaggccgg
     1501 tgtccgccac cgtcagcttc acgtacttgc ccggcgcgat cccgcggccc ccgcgctcgt
     1561 gcagcgtctc gctcgacgtg gcgacggtga ggatccctcc gtccggcatc gcgtcgcgcg
     1621 cgttcacggc gaggttcaac aggacctgct cgagctggcc cgcgtccgcc ttcacgtccc
     1681 acaggccggg tgcgcagtcg acctcgaggc tgatgtgctc gccgatggca cgggcgagga
     1741 tctcccggac gccttctacg acctcgttga gcccgaccgg cccgtgctcc accgcgtcgt
     1801 ggcgggagaa cgcgaggagc tggtgcacga gcttggccgc tcgtcgtccg gcgtcgacga
     1861 tctgctcgac gtcggcgcgg gacgcgtcgt cggaggggag cgcctcgagc acgaagtcgc
     1921 cgtagttgag gatgaccgcg aggatgttgt tgaagtcgtg cgcgacgcct ccggcgagcc
     1981 gcccgaccgc ctccatcttc tgggcctgcc ggagctgggc ctcgagcatc gccttctcct
     2041 cctccgccct gaccctgtcg gagatgtcgg tggagatggc gacgagagca tccggcgtgc
     2101 cgtcctcgtc gaacaggggg aacttgaccg agaggtacgt cccccggcgc ccggtggcgt
     2161 cgggcacgat ctcctcgacc tcgacgggct ccccgcgcgc gaggacctcc cgatccatcg
     2221 ccgacagcgt ccccgcgacg tcttcgggca ggaggtcgtg atcggtcttc ccgcgcaagt
     2281 cgtcgccgtg ggcaccgatg atctgtgcga accgggagtt cgtcagcacg tagcggccgt
     2341 ccgtgtcctt gatgtagatc gccgcggggg agttgtcgat gacctcctgg aggagggcct
     2401 ggctctcccg cagcgccttc gccgcatgct cgcgctccgt cacgtcggag acgtggctga
     2461 cgaagtacag cggcgcgccc ctctcgtcgc ggatgagcgc gtcgttgatc tcgacctgga
     2521 tcacgcggcc gtcgcgacgc acgtagcgct tggacacgcg cgtgacctgc tcgcggcccg
     2581 acttgaagtt ctcgacgtcc tcgatgctgc gcgcccggtc gtccgggtgc gtcaacgtca
     2641 cccagtcgag cctgaggagc tcgtccttcg gatagccgac gatctcgcag agcgcctcgt
     2701 tgacgtcgac gtaggcgttg gtgaccgcgt cgctgagggc gatgccggtg gtggccgcgt
     2761 cgaacgcgcc gcggaacagg gcctcccgct cgcgcagctc acgctccatc gcctgccggt
     2821 gcgtcgcgtc cttgaacgcg acgaccgcgc cgctggggcg cccctctcgc atgatcgggt
     2881 gggccgagaa ctcgaccggg aagctgctcc cgtcacgacg ccggtagacc tcgtcgtccc
     2941 gcgacgacgc ctgtccggac ctgaaggccc gcgcgagagc ggacgcgccc tcgggccccg
     3001 gggccgcggc ggtgtggtgg acgagctcgt ccatcgccgt ccccatgacc tcctcgcggt
     3061 catggcccag catccgcaga gcggcgccgt tgacgaaggt gcaccgcccc tcggcgtcga
     3121 cgccgaagat cccctcgccg gtcgactcga gaaggaggct cagctgctcc gcgagagctt
     3181 cctgacgccg gtagagacga gtccgctcct cgtccgcagc gcgcctctcc gtcacgtcct
     3241 gcgcggtgcc gacgatcctc ggtccggcgt ccgtaacacc cgggacgccc cggacgtgta
     3301 tccaccgcac gcacgcgtcc gcccgcacga tgcggtattc gccctcgaac gcggtgccgc
     3361 tcacgaggga gtcctggacg agcgcggcga ccaccggacg gtcctcgtcg tggaccctgc
     3421 gaaggaagct ctggtagtcg aggtcgacgt cccccggctc gtgtccgaag atcctgaaca
     3481 tctcggccga ccacacgatc gcgtcgcccg tcacgtccca cgcgaaggac cccacgtgcg
     3541 ccaccgcttc ggcgtccgcc agcagccggc ggctctcgcg cagcacgcgc tcggactcct
     3601 tccgctccga tacgtcgtgg tagttcagga tgattccgcg gatcgccgga tcgtcgagcc
     3661 ggttggcggc gcgggcctcg atccatatcc acccgccggt gaccgtcgcg gcccgcacct
     3721 ccacggtctt ggtcctcgcc gggtcggcga cgacgtccgc caggtgcgtg acgaggccgg
     3781 cccggtcctc ggggtggacg aagtcgaacc cggaccggcc gacgacgtcc tccgggtcgt
     3841 gccccgacag cgctttcacg ctcggactga tgtaggtgca cgtccctgtc tcgtcgatca
     3901 ccgtgatcac gtcgcgcgag ttctgtacca gcgagcggaa ccgctcttcg ctctcgcgca
     3961 ggctctgctc catcgcgcgt tgccgccggc gcaggtcctc ctgctcgaga acgcccagca
     4021 ccgcccgcgg caagcgggcc atccggtcct tcacgacgta gtcgccggcc cctcgcttga
     4081 tgcactcgac ggcgatctcc tcgtcgacgt tgcccgtgac gacgatgaac gggaccgcgt
     4141 gcccgcggct ctcggcgatc tccatcgcgg tgtaggggtc gaagctcggg aggttgtagt
     4201 cgaccagcac cacgtctgcg tcctccaaac ccgcggtgaa gtcgtcgcgg gtcaccgccc
     4261 gcgtgacgtg gacggagagg ccaccggcta cgagctcgcg ttcgaccagc gcggcgtcga
     4321 acgggttgtc ctcgacgagt aggactcgga tcggcgcggg gatgctcacg gtgcgaccgc
     4381 cctctgcccg cgtgggggtg actggttcag caacagccag tagaagccga tcacccggac
     4441 tgcctcgctg aactggtcga agtcgacggg cttaacgatg tagctgttga cgccgagcga
     4501 gtaggtctcg acgatgtccc gctcggccgc ggacgacgtc aggacgacga cggggatgtc
     4561 gcgcgtcctc tcgtcggcct tgatggcacg caacacctcg aggccctcga ccttcggcag
     4621 cttcaggtcc agcaggatca cttgggggtc cgtggcgctt tcgcggccgg cgtgctcgcc
     4681 actcttgaag aggtattcga gcgcctgttc gccgtccctc gcgacgtgca cgccgttcgt
     4741 caggttgtgc ttcttgaagg cgtggagcgc gagcgcgaga tcgctggggt tgtcctcgac
     4801 caggaggatc tggacgccgt tcatgtctgc ttcgtcccga tcgtgaagca gaagatggca
     4861 cctcgcccgg gctcgccatt gcaccagatg cggccctcgt gcttctcgac gatccgctgg
     4921 acgttcgcga ggccgatgcc cgttccttcg aagcgctccg gctcgtggag ccgctggaac
     4981 accccgaaaa tcttctcggc gtacttcggg tcgaagccca cgccgttgtc gcgcacgaag
     5041 tagacgtcct cgccgccgtc gcggtacgag ccgatctcga ccgcggggtg gggcacgccg
     5101 cgcgtgaact tcaacgcgtt gtcgatcagg ttcgcgaaca cctgcttgag cagcgtcgcg
     5161 tccgccacgc accccggcag gtgtcccgtc gagatttcga cggcgggccg gactgctgca
     5221 ccgaggtcgg acagtgcctg ccgcaccagg acctcgggcg agaccggcgc caggtcgagg
     5281 ccgctccggc cgagccgtga gaacgcgagc agcccgtcga cgagttgctg catcccctgc
     5341 gcgttcgccc ggatgtacga gaggtactgc gcatggtcgt cggagagcga gtcgccgagc
     5401 tcctcgagga ggatcctgct gaacccgtcc agcgcgcgca ggggggcccg gaggtcgtgg
     5461 gacacggagt acgcgaagga gtccagctcg gccatcgact cctctagctc gcgcgtcttc
     5521 tcctggagct ccagcgtccg ctgcaccacg cgctcctcga ggtcgcgccg gagcgcctcc
     5581 gcctcggcct ctgctcgttt ccgatccgag atgtccatga cgatgtgcga ggagccgacg
     5641 acctcgccgg cagggtcgcg gataggcgac accgtcaccc acacctggcg ctcggcccct
     5701 ccggaggtgc ggtgcctcgt ctcgtacctc gtcacgtcgc ctgcgagggc gcgctcgagg
     5761 actcgttgca gctcggcctc gtccgtgacg accgtcgcca gagcgtcccc gaccgcctcg
     5821 tcccggcgac gcccgtagag ggtctctgcc gcggcgttcc agctggtgat cgtcccgtcc
     5881 aggtcgacgc cgacgagcgc gtcgaacgac cactccac
//
LOCUS       DASQDO010000332         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_281068, whole genome shotgun sequence.
ACCESSION   DASQDO010000332 DASQDO010000000
VERSION     DASQDO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281068"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /gene="greA"
                     /locus_tag="VEV43_09340"
     CDS             complement(<1..190)
                     /gene="greA"
                     /locus_tag="VEV43_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733678.1"
                     /note="necessary for efficient RNA polymerase
                     transcription elongation past template-encoded arresting
                     sites; arresting sites in DNA have the property of
                     trapping a certain fraction of elongating RNA polymerases
                     that pass through, resulting in locked ternary complexes.
                     Cleavage of the nascent transcript by cleavage factors
                     such as GreA or GreB allows the resumption of elongation
                     from the new 3'terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HYP23765.1"
                     /translation="MPDQKKVPLTQQALARLQEELDYLQGEGRTKIIEEIATARAHGD
                     LSENAEYHAAREQQGLQEA"
     gene            223..993
                     /locus_tag="VEV43_09345"
     CDS             223..993
                     /locus_tag="VEV43_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP23766.1"
                     /translation="MSGRLQDKVCIVTGSGRGIGRAIVLAFAAEGAHVVVNDLDADAA
                     KETANDVTSQGGRAEVNTDAIGTVEAAESLVATALDAFGRVDVLVNNAGILRDKMLHN
                     MTEEDFDLVIKVHLKGTWACGRAVVQHWRPLAKQEAEPPHRKIVNVTSPSGLLGAVGQ
                     SNYAAAKMGIVGLTKTWAKELGRLRINVNAVSPTALTAMTEPLLEDPVAAEKRLARFA
                     LGRYGAPEEIAPAFVYLASKESDYVTGQVLCVDGGLVI"
     gene            993..1409
                     /locus_tag="VEV43_09350"
     CDS             993..1409
                     /locus_tag="VEV43_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23767.1"
                     /translation="MERAWPFEGVPEGGQNLRSPEDFLAAYGEGTEAVVVRIGLHDAQ
                     LVLVDARGGWQRWVYHSVEEATEVAERLGVPVHAGEYPEELRVKMNAFQRPSEDFDRR
                     AYPEQGAVGPVIPYPENRPRRLEDPRREADRPGSKP"
     gene            complement(1330..1833)
                     /locus_tag="VEV43_09355"
     CDS             complement(1330..1833)
                     /locus_tag="VEV43_09355"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HYP23768.1"
                     /translation="MGPLIFLFLLAMPIVELAVIVRVAEQVGVLETLVLLVAISIAGA
                     WLLKQQGLATWKSLQQTMARGEMPNKEVTDGALILFGGALLLTPGFVTDAFGLVLLLP
                     PTRALVKSSFRKLFARWATRRAGRAGVVYSATVKNVRRTPTASNRAGRLPGEDPPGDE
                     GGSPGTG"
     gene            1875..2474
                     /locus_tag="VEV43_09360"
     CDS             1875..2474
                     /locus_tag="VEV43_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYP23769.1"
                     /translation="MTVTTTVKRLTFDEALEVVEMGGAFVDLRPTAAYLDAHVPGALD
                     VVYESGPGMSTRARDCLPLSIPYVLLDLGHGDLSLAAAALRGKGFSVVGSVQDGLNQW
                     AAKGRRIASTEIVTGETAPPGTVLHVADPGAKVPEEAVQIPADDLWPRCDELDRANPV
                     VVVAGWGVRAGLAIGILERNGFEDVVMWRPGGGHRGGSV"
     gene            complement(2471..>2851)
                     /locus_tag="VEV43_09365"
     CDS             complement(2471..>2851)
                     /locus_tag="VEV43_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYP23770.1"
                     /translation="EHEWVTLMARRMSERRRKDRLNSDGALARQAAQLADRYLDGVRP
                     ASISWVTNQQSRWGSCCPEDRTIRLSIALADYPAWVRDYVIVHELAHLVVPDHSPRFW
                     DLVNRYPLTERARGFLIAKGMAEG"
BASE COUNT          438 a          979 c         1024 g          410 t
ORIGIN      
        1 gcgcctcctg caacccctgc tgctcgcgcg cggcgtggta ctcggcgttc tcgctgaggt
       61 ccccgtgcgc acgcgcggtc gcgatctcct cgatgatctt cgtgcgcccc tcgccctgga
      121 ggtagtcgag ctcttcctgc agacgtgcga gagcctgctg cgtgagcggg accttcttct
      181 gatcgggcat cggtcgatcc tagtcgcata ggatcgccgc caatgtctgg gaggttgcag
      241 gacaaggtct gcatcgtcac ggggtccggc cgcgggatag ggcgcgcgat cgtgctcgcg
      301 ttcgcggccg agggcgcgca cgtcgtcgtc aacgacctcg atgcggacgc cgcgaaggag
      361 accgcgaacg acgtcacgtc gcaggggggc cgcgcggagg tcaacaccga cgcgatcggc
      421 acggtcgagg ccgccgagtc cctcgtcgcc accgcgctcg acgcgttcgg acgcgtcgac
      481 gtcctcgtca acaacgccgg gatcctgcgc gacaagatgc tgcacaacat gaccgaggag
      541 gatttcgacc tcgtgatcaa ggtccacctc aaggggacgt gggcgtgcgg tcgtgcggtc
      601 gtccagcact ggcgcccgct cgcgaagcag gaggccgagc ccccgcaccg caagatcgtc
      661 aacgtgacca gtccgtcggg gctcctcggc gcggtcggcc agtcgaacta cgccgccgcg
      721 aagatgggga tcgtcgggct gacgaagacg tgggccaagg agctcgggcg cctgcgcatc
      781 aacgtgaacg cggtctcgcc gacggcgctg acggcgatga ccgagccgct gctcgaggat
      841 cccgtggccg ccgagaagcg cctcgccagg ttcgcgctcg gccgctacgg cgcgcccgag
      901 gagatcgcgc cggcgttcgt ctatctcgcg tccaaggagt ccgactacgt cacgggccag
      961 gtcctgtgcg tcgacggggg cctggtcatc tgatggagcg cgcgtggccg ttcgaaggcg
     1021 tgcccgaggg cgggcagaac ctgcgctcgc cggaggattt cctcgccgcg tacggcgagg
     1081 ggacggaagc ggtcgtcgtc cgcatcggcc tccacgacgc gcagctcgtg ctcgtcgacg
     1141 cgcgcggcgg gtggcagcgg tgggtctatc actccgtcga agaggcgacc gaggtggccg
     1201 agcgcctcgg cgtccccgtg catgcgggcg agtatcccga ggagctgcgc gtgaagatga
     1261 acgcgttcca gcgcccgtcc gaggacttcg accggcgcgc ctatcccgag cagggcgccg
     1321 tcgggccggt catcccgtac ccggagaacc gccctcgtcg cctggaggat cctcgccggg
     1381 aagccgaccg gcccggttcg aagccgtagg cgttcgccgc acgttcttca ccgtcgccga
     1441 gtagacgacc ccggcccgcc ccgcacggcg cgtcgcccat ctcgcgaaca gcttgcggaa
     1501 cgaggacttg acgagcgcgc gcgtcggcgg gagcagcagc acgaggccga acgcgtcggt
     1561 cacgaacccg ggcgtgagca agagcgcgcc gccgaacagg atcagcgcgc cgtcggtcac
     1621 ctccttgttc ggcatctcgc cgcgcgccat cgtctgctgg aggctcttcc acgtcgcgag
     1681 accttgctgc ttcagcagcc acgcgccggc gatcgagatc gcgacgagaa ggacgagcgt
     1741 ctcgagcacg ccgacctgct ccgccacccg gacgatgacc gcgagctcga cgatcggcat
     1801 cgcgagcagg aagaggaaga tcagcggccc catcgagcca gtatggacgc gcgggtctgg
     1861 tatgagtacg cgccatgacg gtgacgacga cggtgaagcg cctgacgttc gacgaggccc
     1921 tcgaggtcgt cgagatgggt ggcgcgttcg tcgacctcag gccgaccgcc gcatacctcg
     1981 acgcgcacgt cccgggcgcg ctcgacgtcg tctacgagag cgggccgggc atgtcgacgc
     2041 gagcgcggga ctgcctgccg ctgtcgatcc cctacgtgtt gctcgacctc ggccacggtg
     2101 acctctcgct cgcggccgcg gcgctgcgcg gcaaggggtt cagcgtcgtc ggctccgtgc
     2161 aggacgggct caaccagtgg gccgcgaagg gacggcgcat cgcgtcgacc gagatcgtga
     2221 cgggagagac ggccccgccc ggcacggtgc tccacgtggc cgacccgggg gccaaggtcc
     2281 ccgaggaggc cgtgcagatc cccgccgacg acctgtggcc acggtgcgac gagctcgaca
     2341 gggcgaatcc ggtcgtcgtc gtcgcgggat ggggcgtccg ggccgggctg gcgatcggga
     2401 tcctcgaacg gaacggcttc gaggacgtcg tcatgtggcg accgggcggc ggccaccggg
     2461 gcggcagcgt ctaaccctcg gccataccct ttgcgatgag gaacccgcgc gccctctcgg
     2521 tcagcgggta gcggttgacg aggtcccaga accgcggcga gtggtcgggg acgaccagat
     2581 gcgccagctc gtggacgatc acgtagtcgc ggacccaggc ggggtaatcg gccagcgcga
     2641 tcgagagcct gatcgtccgg tcctccgggc agcacgaccc ccagcgggac tgctggttcg
     2701 taacccacga gatcgaagcc ggccggacgc cgtccagata gcggtcggct agctgagccg
     2761 cctgacgcgc cagggccccg tcggagttca gccggtcctt gcgacggcgc tcggacatcc
     2821 gccgggccat gagcgtgacc cattcgtgct c
//
LOCUS       DASQDO010000333         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_219428, whole genome shotgun sequence.
ACCESSION   DASQDO010000333 DASQDO010000000
VERSION     DASQDO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219428"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VEV43_09370"
     CDS             <1..106
                     /locus_tag="VEV43_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYP23771.1"
                     /translation="LLGHSSLATTQVYTLVSDRHMHEVYVTSHPRARA"
     gene            140..490
                     /locus_tag="VEV43_09375"
     CDS             140..490
                     /locus_tag="VEV43_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYP23772.1"
                     /translation="MDEQALKTIKARLEEERASVERQLHEHGAEVDGGGIEVGFDGGF
                     ADSGHRTAETAEVLALVEQLAAHRREVVDALDRIEAGTFGTCERCGNQIPDERLEARP
                     TAALCVACKQATGG"
     gene            581..1747
                     /locus_tag="VEV43_09380"
     CDS             581..1747
                     /locus_tag="VEV43_09380"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HYP23773.1"
                     /translation="MALVVLDSVGIGGAPDAAAFGDEGSSTLPHVAAAVGGIDLPNLG
                     ALGLGRVADIPGVPPAADPRGAFGAMVETSPGKDTTTGHWEIAGVVLERPFPTYPDGF
                     PPEVMDAFEAAIGFSTLGNVAASGTEIIERLGAEHLATGLPIVYTSADSVFQVAAHVD
                     VVPLETLYEMCRVAREILRGEHEVGRVIARPFEGSPGSFRRTPDRHDFSVLPPAPTVL
                     DAVAGAGQEVRGVGKISDIFAGRGVTGSRPTRSNDEGVTAVVDELRAIGRGLVFANLV
                     EFDSSFGHRNDPQGYAAALEAFDRRLPEIVDALGSRDVLIVTADHGNDPTTASTDHSR
                     ERVPLLAVGEPVRAGASLGERGSFADCGATVADLLGVPAPPAGESVAKELLHRA"
     gene            1919..2884
                     /locus_tag="VEV43_09385"
     CDS             1919..2884
                     /locus_tag="VEV43_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP23774.1"
                     /translation="MAPAGVAAGPLQDQAAANGAASVVDGGATAASDLPAPGDAEAAA
                     PGGPARAPWASDPRRSARVIAMANQKGGVGKTTTTISLGAALAELGQKVLVVDFDPQG
                     GCALGLGIEPGALELSVYNALLDRNCPSEDVVIQTNVPGMDLLPSNIDLSAAELMLVQ
                     EVAREQSLSRALAPLRRSYDFILVDCPPSLGLLTINALTAADGVIVPLECEYYALRGM
                     ALLMDSIERVRDRLNPQLEIDGILATMYDGRTLHAREVLERVRDAFGDTLFDSIIRKT
                     IRFAEAPVAGEPILSYAPTSTGSASYRALAKEVISRVSTRQPAGS"
     gene            2853..>3083
                     /locus_tag="VEV43_09390"
     CDS             2853..>3083
                     /locus_tag="VEV43_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HYP23775.1"
                     /translation="MSRRASLPGADELFRPTASPRRGTAAEREDKSPRSQGEKSTNLQ
                     VAEATSAGRKAPRHETKVTFYCTNGDLTALDQA"
BASE COUNT          455 a         1092 c         1119 g          417 t
ORIGIN      
        1 gcttctgggc cattcgagcc tcgcgacgac gcaggtctac acgctggtca gcgatcgtca
       61 tatgcacgag gtgtacgtga cgtcgcaccc gcgcgcccgc gcctgagaga cgcgttacgg
      121 tcacgcccga gggggagaca tggacgaaca agcactgaag acgatcaagg cccggctcga
      181 ggaagagcgc gcgagcgtcg aacgccagct ccacgagcac ggggccgagg tcgacggcgg
      241 cgggatcgag gtcgggttcg acgggggctt cgccgactcg ggtcaccgga cggccgagac
      301 cgccgaggtg ctggccctgg tcgagcagct ggcggcgcac cggcgcgagg tcgtggacgc
      361 cctggaccgg atcgaggccg ggaccttcgg cacctgtgag cgctgcggca accagatccc
      421 cgacgagcgc ctggaggccc gcccgacggc ggcactgtgc gtggcgtgca agcaggcgac
      481 ggggggctga gcccgccttg ccgtgaccgc tgatcacggc cgggtcggag ccgctccggc
      541 ggcgggcccg gacggcgatg gtgccgggct cgtccgggct gtggctctcg tcgtcctcga
      601 ttccgtcggc atcggcgggg cgccggacgc ggccgcgttc ggcgacgagg gttcctcgac
      661 gcttccgcac gtggccgctg cggtcggggg gatcgacctg ccgaacctcg gggcgctcgg
      721 gctcgggcgg gtcgcggaca tccctggggt cccgccggcg gccgacccga ggggcgcgtt
      781 cggggccatg gtcgagacgt cgcccggcaa ggacacgacg accgggcact gggagatcgc
      841 cggggtcgtg ctcgagcggc cgttcccgac gtacccggat gggttcccgc ccgaggtcat
      901 ggacgcgttc gaggccgcta tcgggttctc gacgctcggc aacgtcgccg cctccggcac
      961 cgagatcatc gagcggctgg gggccgagca cctcgccacc gggctcccga tcgtctacac
     1021 gtcggccgac agcgtgttcc aggtcgcggc ccacgtcgac gtcgtgcccc tggaaaccct
     1081 ctacgagatg tgtagggttg cgcgggagat cctgcggggc gagcacgagg tcggccgggt
     1141 gatcgcccgg cccttcgagg ggagccccgg ctcgttccgc cgcacgccgg atcgccacga
     1201 cttctcggtg ctgccgccgg ccccgacggt gctcgacgcg gtcgccgggg cggggcagga
     1261 ggtgcggggc gtcggcaaga tctcggacat cttcgccggg cggggggtga cgggctcacg
     1321 gccgacccgg tccaacgatg agggcgtcac cgcggtggtc gacgagctga gagcgatcgg
     1381 ccgcggcctc gtcttcgcga acctcgtcga gttcgactcg tcgttcggcc accgcaacga
     1441 cccgcagggc tacgccgcgg ccctcgaggc gtttgaccgg aggctcccgg agatcgtcga
     1501 cgccctcggc tcacgcgacg tcctgatcgt cacggccgac cacggcaacg acccgacgac
     1561 ggcgtcgacc gaccactcga gggagcgggt gccgctgctc gcggtggggg agccggtccg
     1621 ggctggagcg tccctggggg agcgagggtc gttcgccgac tgcggggcca cggtcgcaga
     1681 cctgctcggc gtcccggccc cgccggcggg cgagtcggtc gcgaaagaac tgttgcaccg
     1741 agcctgaagt gcctcttgtg ccccgttcgg gcgcgggata tcgtccacac ccctacaaga
     1801 cgacttgtcg acaagtcgcc acgacccggc ggcggagagg ggatcgatgg agggagacgt
     1861 ccgaacggcg gaccgctcgg ccccgtcgga agcaccggtc caggtaacac tccccgacgt
     1921 ggccccggcc ggcgtagcgg cgggcccgct ccaggaccaa gcggcggcga atggcgccgc
     1981 ttccgtcgtg gacggcggcg caacggccgc ctccgacctg cccgcccccg gtgacgccga
     2041 ggctgcggcc cccggcgggc cggcccgggc cccgtgggcg tccgaccccc gccgcagcgc
     2101 acgcgtcatc gccatggcga accagaaggg cggcgtcggg aagacgacca cgacgatcag
     2161 cctcggggcg gccctcgccg agctcggcca gaaggtcctc gtcgtcgact tcgaccccca
     2221 ggggggatgc gctctcggcc tcgggatcga gcccggcgcc ctcgagctct cggtctacaa
     2281 cgccctgctc gaccggaact gcccgtccga ggacgtggtg atccagacca acgtgccggg
     2341 gatggacctc ctcccgtcga acatcgacct ctcggcggcc gagctcatgc tcgtacaaga
     2401 ggtcgcccgc gagcagtcgc tctcccgggc cctcgctccg ctccgccgga gttacgactt
     2461 catcctcgtg gactgcccgc cgtcccttgg cctcctgacg atcaacgcgc tcaccgcggc
     2521 cgacggagtg atcgtcccgc tcgagtgcga gtactacgcg ctccggggga tggcgctcct
     2581 gatggactcg atcgagcggg tgcgggacag gcttaaccct caactggaga tagacggaat
     2641 ccttgccacg atgtacgacg gccgcaccct ccacgcgagg gaggtgctcg agcgggtccg
     2701 cgacgcgttc ggcgacacgt tgttcgactc gatcatcagg aagaccatcc gcttcgccga
     2761 ggcgccggtg gcgggggagc ccatcctcag ctacgcgccg acgtccaccg gctcggcctc
     2821 ctaccgggcc ctcgccaagg aggtgatcag ccgtgtctcg acgcgccagc ctgccgggag
     2881 ctgacgagct gttccggccg acggcgtccc ctcgcagggg gaccgccgcg gaaagagaag
     2941 acaagtcgcc tcgtagccaa ggcgagaagt cgacaaatct acaagtagcc gaagcgactt
     3001 ctgcgggccg caaggccccc aggcacgaga ccaaggtgac gttctactgc acgaacggcg
     3061 acctcacggc gctcgaccag gcc
//
LOCUS       DASQDO010000334        13621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_464298, whole genome shotgun sequence.
ACCESSION   DASQDO010000334 DASQDO010000000
VERSION     DASQDO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13621
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464298"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..544)
                     /locus_tag="VEV43_09395"
     CDS             complement(158..544)
                     /locus_tag="VEV43_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HYP23776.1"
                     /translation="MVEPRVDTPPAVTFEDLVSEAIDSLPLELAVRMDNVEVVVEDEP
                     PPEMIARLAPGSTLLGLYHGVPLTQRGINYDRMLPDKISIYRGPIVRMARTPDRIREQ
                     VRRTVIHEIAHHFGIDDDRLHELGWA"
     gene            662..1210
                     /locus_tag="VEV43_09400"
     CDS             662..1210
                     /locus_tag="VEV43_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23777.1"
                     /translation="MRRRRRSGWLILLVVLAVFSAAGSVQAQSVPGNAGNLDDGGPVD
                     PADVLALRGADASWIPTAGEMLQADCEASVDPPEFFEEDGVVRVRGWGHWSCNRALPG
                     SYLEVCLDAVVPSVACNEKFVPPPRTAFSVKVDFPCVPGVYVTMATGSNPFDTNRSND
                     TAHAPLALIVLPADCKYVQAGP"
     gene            1207..1800
                     /locus_tag="VEV43_09405"
     CDS             1207..1800
                     /locus_tag="VEV43_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23778.1"
                     /translation="MRRRGVVVAAALVVAASMAVPAARAGVYAAEHTRAVAYWYLPTA
                     DDDVYRLYRAEVVSVRNLLTGAVSSQASIITDRCTEKPIAPNQTLFACGDGRRERTTR
                     DAELEIAADMSSGTARIRLRGKTHVVYFEGPAEGGAYEMYDSCSETDHRVVAGTFTNM
                     DRAHGRVLGKRLDRPVGSDLDHAWLMRGFGTWCDEDG"
     gene            complement(1811..3532)
                     /gene="treZ"
                     /locus_tag="VEV43_09410"
     CDS             complement(1811..3532)
                     /gene="treZ"
                     /locus_tag="VEV43_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HYP23779.1"
                     /translation="MEFRVWAPAPERVELVLRGERHGMSRDDRGWWSADADASHGDGY
                     GFSLDGGDPLPDPRSPWQPGGVHGFSRVVDHSSYEWRDADWRGRPLASAVVYELHTGT
                     FTPEGTFESAIERLDHLVRLGVTHVELLPVAQFPGDRGWGYDGVDLFAPHESYGGPEG
                     LKRLVDACHERGLGVIMDVVYNHLGPAGNYLGSYGPYFTDRYATPWGQAVNFDGPGSV
                     EVRRFFVDNALMWLRDYHCDGLRLDAIHAILDASAIHVLEEMQLRVEELEAHLDRDLF
                     LIAESDLNDPRVVNRREAGGYGLDAQWSDDFHHALHSVLTGETDGYYADFGTIGHVAK
                     ALREAFVHAGTYAEYRGRIHGRPAVGLPGYRFLGYLQDHDQIGNRAVGERSSALMSPD
                     LLKVGAALVLTSPFVPMLFMGEEWGATAPFLYFTAHEDAELGKAVSEGRKREFAAFGW
                     DPDSIPDPQDRSTYERSKLDWGELDEQPHADLLDWHRSLIALRREWPELTDGRLDRVE
                     VDYDEDGRWLTTTRGRVTVECNLGPSEVEAPVGAEGRRILLTSGKEPAVSGGSVRLPA
                     ESVTIWG"
     gene            complement(3532..5997)
                     /gene="treY"
                     /locus_tag="VEV43_09415"
     CDS             complement(3532..5997)
                     /gene="treY"
                     /locus_tag="VEV43_09415"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HYP23780.1"
                     /translation="MPKDVRATYRLQLNAGFTFDDAAGVTGYLARLGVSHAYCSPYLQ
                     AAAGSAHGYDVTDHSKVNDELGGPDAHERFCAALSANGLGHVLDVVPNHMCISDASNA
                     WWWDVLKHGASSRYAGYFDIDWDPPEAMLHRTILVPILGDHYGRVLEAGELRLAAEDG
                     EVVLRYHEHVLPVSPGTAPDDLDAVNADPDALHEVLDKQHYRVAFWKSGDRDLNYRRF
                     FDIKTLAALRIEDERVFEDTHRLVLSMIEGGNLDGLRIDHVDGLRDPGAYLEKLRSRS
                     PEAYLVVEKILEAGEELPETWPVEGTTGYDFLNVLNGLFVDSNAEKAMSDVYLDFTRE
                     PADLAEMIRAKKLFIMREVLVSDIERLTALFVEVSESHRRYRDYTRHELREAIRETIA
                     AFPVYRTYLDTRTRTPSPQDVAHVQEAVEEAAARRPDLAPELFEFLCNVLLMRYSGPA
                     EDAFVLRFQQTTGPVMAKGVEDTVFYNFNRLVSLNEVGSDLGCFGVSLEAFHDRAVRA
                     QERWPLAMLATSTHDTKRSEDVRARIDLLSEIPAQWGDAVTRWKAHNDRHRSGGIPDR
                     NAEYLLYQTLVGAWPLPADRAVAYMEKASKEAKAHTAWTDPSPEYDEGLAAFVRAVLD
                     DREFVAELEDFAAPLVEPGRINSLAQTAIRLTYPGVPDVYQGSELWDLSLVDPDNRRP
                     VDYEVRARLLEEVLAGDAAGALARMDEGLPKLLLTQRVLALRERDPAAFGEGSAYEAL
                     WATGPRARHVVAFTRAGRVLTVAPRLVLTLEGAWRDTHLALPEGDWTDTLTGTTFSGV
                     VPLEELLGGFPVLVATRTEGGSV"
     gene            complement(5999..8125)
                     /gene="glgX"
                     /locus_tag="VEV43_09420"
     CDS             complement(5999..8125)
                     /gene="glgX"
                     /locus_tag="VEV43_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HYP23781.1"
                     /translation="METWPGGPFPPGATWDGGGSNFSLYSENADAVELCLFDDSGAET
                     RLGVTESTAHYWHAYVPGVGPGQRYGYRVHGPWKPQDGLRFNPTKLLLDPYALAIDGD
                     VDWGPEVFAYKMGDSAEDLSFDERDDGARVPKSVVVDGSFYWGDDKHPNTPWHESVIY
                     ETHVKGFTITHEGIPPEIRGTYAGLAHPAAIEHLQLLGVTAVELLPVHHFVHPQMLID
                     KGLRNYWGYDSIGYLAPYSGYSSSGTRGEQVTEFKQMVKSLHEAGIEVILDVVYNHTA
                     EGNHLGPTLSFKGVDNSVYYRLVPDDRRFYFDTTGTGNSLNVRHPEPLKVITDSLRYW
                     ATEMHVDGFRFDLAATLARQFHEVDRLSAFFDIIHQDPVLSRTKLIAEPWDVGEGGYQ
                     VGNFPALWSEWNGKYRDTVRDFWRGEDSRLGEFASRFTGSSDLYQDDGRHPNASVNFI
                     TAHDGFTLHDLVSYNDKHNEDNGEGNRDGDNHNRSWNHGEEGETDDPAIRSLRERQKR
                     NFLATLMLSQGVPMLLGGDEIGRTQRGNNNAYCQDNEISWFDWALRDENLALLGFTRR
                     LMDFRRRHPIFRRRKWFMGREIHGSGVRDIGWFSPTGEEMGDDEWTAGFAKSIAVFLN
                     GDEIPQRGPKGERITDDSFLLLFNAHHDTIEFTLPSEAWGPRWTAELDTAEALGTPAE
                     ATHEAGAKLPVTGRSLVILQRVDESA"
     gene            complement(8262..8894)
                     /locus_tag="VEV43_09425"
     CDS             complement(8262..8894)
                     /locus_tag="VEV43_09425"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HYP23782.1"
                     /translation="MNVRATFLETGRVAADLLARPEVEAAWDSPSALPEFTVRGLAGH
                     LLRATGSVAAYLDRPEPSGEPVSAAEYYAQAVDEPDITSEIHRAIRQRGEDEAAGGFA
                     SVRDRSYELLETLRTRLEAEPPDRKVEAYKGIVLTIDEYLVTRLIELVVHVDDLSVSV
                     GVEAPPLPPDATKLAIDALVDVARQKHGDLAVLRALTRRERDEAGALRVL"
     gene            complement(8891..10060)
                     /locus_tag="VEV43_09430"
     CDS             complement(8891..10060)
                     /locus_tag="VEV43_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23783.1"
                     /translation="MTGYRLSFARRDLAIVLILAIGVALRFWVSTLGSNFDLESYGIV
                     AEIVDRGGNVYAETERYNYGPIWFFVLGVLHDLGGLFSDPVGAFRILIIAVLTLADVG
                     IWAVLHRRYGRIAGFLFFLNPISIIITGYHNQFDNLAVLVGLLAMVVYAASFLPALLL
                     LGLSLTVKHVLLVLPLWLAIREKGLRRKLLAVAVPLSVLAVSFVPFLAGGRSGIVSNV
                     ALYRGHDNAPFWHGVLPAQLQPLISPIVLFGVVVLVLGFVWRREEPLDLALLYLVTIV
                     VFAPSIANQYLAIVVPALAAFPSPLFLPYQVLAAWMLAIHPDGLHNAWLRDRSPGQLV
                     AEQSWPRTYDPLIMALSLGLAGLLFPALHSLPRRAWEWTSREAKAQWRLLRGHDA"
     gene            complement(10107..11369)
                     /locus_tag="VEV43_09435"
     CDS             complement(10107..11369)
                     /locus_tag="VEV43_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYP23784.1"
                     /translation="MRVGLVGAGATGLSAGYTLANAGHDVTVLEKGPELGGLVASIEV
                     GGTPVERYYHHVFASDKALVGLVEELGLGDSLRFRSPTTGIYYDGRLHPFGTPREMLA
                     FPPLGPLDRVRFAASAAYLKALKRYEKLEGVEALAWTSKHAGRRATEVVWEPLLRGKF
                     GERAPEISMAWLWARIHHRTFRLGYLHGGFHRLYAALAAAIENRGGVIRFEKAVERIE
                     RSSGAVRVATSNGTAEEFDRVIVTTPPVELARAIGAPPAGRSPEYLGATCFLLELRRS
                     LVPYYWLNVNDPSFPFLAVVEHTRMEDPATYGGRHVVYVGNYVARDDPRYTAEPEKLL
                     DEYLPYLQRLSPGFGLDDVLDWHFSRAPFAQPVVTVGYRSTIPPHTTPVEGVYLASMA
                     QVYPQDRGQSYAIAMGRDLARRISAGTI"
     gene            complement(11380..12168)
                     /locus_tag="VEV43_09440"
     CDS             complement(11380..12168)
                     /locus_tag="VEV43_09440"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYP23785.1"
                     /translation="MTRCDDVRLDFHSYLMGELAPPAAQEVRRHVDGCDECRGELGRV
                     AGVHKLLVDAPLEHAPGPLLEEDVFAFVELDRVGRLASQVPLSVEPPDGLERAALERA
                     GIGTPRRSRATTVLVPALGVATVVLGVLGINWRSQADRMREDLGPAGQTMQLVELQGD
                     SVPPEDVELVRYGGAGYRVEMDGPIPPAPDGYYYELWMVGASGRVSCGTFVAGDPGGV
                     HFPVGVDPADYPYLQITLEPEDGDESMNGDVMWEGRLDSTASLP"
     gene            complement(12165..12743)
                     /locus_tag="VEV43_09445"
     CDS             complement(12165..12743)
                     /locus_tag="VEV43_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23786.1"
                     /translation="MATGVPGGQALRVDEDQLLQSVARGDVDALRALYRSFERPLYAL
                     GIRWLNDPKLAEELVQEVTLRVWRRAGRYDPERGASSSWIFGIARNVAADLARARSKA
                     PMPVAEPPEGTAPWDEDRAWTAWEVARAVARLPEDQRRVVELAYANQMTQSEIAAELG
                     IPLGTVKTRLYAGLRKLRAIFVERGIAPEGAA"
     gene            12810..13535
                     /locus_tag="VEV43_09450"
     CDS             12810..13535
                     /locus_tag="VEV43_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HYP23787.1"
                     /translation="MPTADPAALRRLLELQNEDTAIKRLEERRASLPEAQRLAEVNAA
                     LAELDADLAIARKQHDEVAREVARLEGEAGLLDQKIAREEQRMFSGGVSNPKELSALQ
                     AEVESLKRRKSGLEDELLEAMLGREQATATIDGLTKERDEAATEAADLNGVVEGITGE
                     ISGELETHTTARTAISGEIPADLLKLYEQLRAQKGGVGAAGLEGGTCLGCHTKLPQRE
                     LERMRAEGGLQRCDNCRRILVID"
BASE COUNT         2098 a         4778 c         4712 g         2033 t
ORIGIN      
        1 gacccgatcg aagagtgcat ctacgcgggg cccccgccct gcatcccggt cagcgtcccg
       61 actccggcaa tctggtgcgg cggctacctg tcgtgccagg tcgtcgaccc gtggcactga
      121 gaagtcgtcc ccactatcgg aaccgagctg ggctgggtta ggcccagccc agctcgtgca
      181 gcctgtcgtc gtctatcccg aagtggtgcg cgatctcgtg gatcaccgtg cggcggacct
      241 gctcgcggat ccggtcgggc gtccgcgcca tcctcacgat cggcccccgg tagatcgaga
      301 tcttgtccgg gagcatccgg tcgtagttga tgccccgctg cgtgagcggg acgccgtggt
      361 agaggcccaa cagggtcgag cccggggcga gccgcgcgat catctcgggc gggggctcgt
      421 cctcgacgac gacctcgacg ttgtccatcc ggacggcgag ctcgagcggg agcgagtcga
      481 tcgcttcgga gacgaggtcc tcgaacgtta cggccggcgg ggtgtccaca cgtggctcaa
      541 ccacgcgacc aagccgaaca atcccgccag aactttcagc gtgtttcggg agggtttgat
      601 ataacaaccg acgaaacaat gcgcaacatc cgaggcactc accggcgtgg gggagatggc
      661 ggtgcgcaga cggcggcgga gtgggtggct gatccttctg gttgtcctgg cggtgttctc
      721 cgcggcgggg tccgtacagg cgcagtccgt tcccgggaac gcgggcaacc tcgacgacgg
      781 aggtcccgtc gacccggccg acgtccttgc gctgcgcggc gcagacgcga gctggatccc
      841 aaccgcgggc gagatgcttc aggcggactg cgaagcgagc gtcgatcccc ctgagttctt
      901 cgaggaagac ggggtcgtcc gggtccgcgg atgggggcac tggtcctgta accgggccct
      961 gcccgggagc tatctcgagg tctgtctcga tgccgtcgtg ccctcggtgg cgtgcaacga
     1021 aaagttcgtc ccgccgccgc gcacggcgtt ctcggtgaag gtcgacttcc cgtgcgtccc
     1081 gggggtctac gtgacgatgg caaccgggag caacccgttc gataccaatc gctcgaacga
     1141 caccgcgcac gctcccctcg ccttgatcgt tcttccggcc gactgcaagt acgtgcaggc
     1201 ggggccgtga ggcgacgcgg ggtggtcgtt gcggccgctc tggtagtcgc ggcctcgatg
     1261 gccgtacccg cggcccgagc cggtgtctac gctgccgaac atacgagggc ggtcgcctac
     1321 tggtacctgc cgaccgcgga cgacgacgtc taccggctgt accgggcgga ggtcgtgagc
     1381 gtacggaacc tcttgaccgg cgcggtctcc tcgcaagctt cgatcatcac cgatcgatgc
     1441 accgagaagc cgatagcgcc gaaccagaca ctcttcgcgt gcggcgacgg ccggcgcgaa
     1501 aggaccacac gcgacgccga gcttgagatc gcggcagaca tgtcgtcggg gacagcgcgc
     1561 atccgcctgc gcggcaagac gcacgtcgtc tacttcgagg gccccgcgga gggaggagcg
     1621 tacgagatgt acgactcctg ctcggagacc gaccaccggg tcgtcgccgg caccttcacg
     1681 aacatggacc gggcccacgg acgcgtcctc ggcaagaggc tcgaccggcc cgtcggctcc
     1741 gacctcgacc atgcgtggct catgcgcggg ttcgggacgt ggtgcgacga ggatggatag
     1801 ggactaggcg ctagccccag atcgtgacgc tctcggccgg gagccggacc gagccccccg
     1861 agaccgccgg ctccttgccg gacgtcagca ggatccggcg accctcggcc cccacggggg
     1921 cctcgacctc cgacgggccg aggttgcact ccaccgtgac ccggccccgg gtcgtcgtca
     1981 gccaccgtcc gtcttcgtcg tagtcaacct cgacgcggtc gaggcggccg tcggtcagct
     2041 ccggccactc ccgtcgcagc gcgatcaacg aacggtgcca gtcgagcagg tccgcatgcg
     2101 gctgctcgtc gagctcgccc cagtccagct tcgaccgctc gtaggtcgag cggtcctggg
     2161 ggtcggggat cgagtccggg tcccagccga aagccgcgaa ctcgcgcttg cggccctcgc
     2221 tgacggcctt ccccagctcg gcgtcctcgt gggccgtgaa gtacaggaac ggcgcggtgg
     2281 cgccccactc ctcgcccatg aacagcatcg gcacgaacgg cgacgtgagg acgagcgcgg
     2341 cgccgacctt cagcaggtcc gggctcatca gcgcgctgct gcgctcgccg acggcgcggt
     2401 tgccgatctg gtcgtggtcc tgcaggtagc cgaggaagcg gtagccggga aggcccacag
     2461 cgggccgccc gtggatgcgc ccgcggtact ccgcgtacgt ccccgcgtgc acgaacgcct
     2521 cgcgcaacgc cttcgcgacg tggccgatcg tgccgaagtc cgcgtagtag ccatcggtct
     2581 ctccggtcag caccgagtgg agcgcgtggt ggaagtcgtc gctccactgc gcgtcgagcc
     2641 cgtagcctcc ggcctcgcgc cggttgacca cgcgcgggtc gttgaggtcg ctctccgcta
     2701 tcaggaacag gtcgcggtcg agatgggcct cgagctcctc gacccgcagc tgcatctcct
     2761 cgaggacgtg aatcgccgac gcgtcgagga tcgcgtggat cgcgtcgagc ctcagcccgt
     2821 cgcagtggta gtcgcgcagc cacatgagcg cgttgtcgac gaagaaccgc cgcacctcga
     2881 cgctgcccgg cccgtcgaaa ttcacggcct ggccccacgg cgtcgcgtag cggtcggtga
     2941 agtacgggcc gtacgagccg aggtagttcc cggccgggcc gaggtggttg tagacgacgt
     3001 ccatgatcac gccgagcccc cgctcgtggc acgcgtcgac gagacgcttg aggccctccg
     3061 gcccgccgta ggactcgtgc ggagcgaaca ggtcgacgcc gtcgtagccc cagccgcggt
     3121 cgccggggaa ctgggcgacg ggaaggagct cgacgtgcgt gacgcccaga cgcacgaggt
     3181 ggtcgagcct ctcgatcgcg gactcgaacg tcccctccgg ggtgaacgtc ccggtatgca
     3241 gctcgtagac gacggccgac gcgagggggc gcccgcgcca gtccgcgtcg cgccactcgt
     3301 acgaagagtg gtcgaccacc cgagagaacc catggacccc tccgggctgc cacggggagc
     3361 gggggtcggg gagcgggtcg ccgccgtcca gggagaaccc gtagccgtca ccgtgcgaag
     3421 cgtccgcgtc ggcagaccac catccccggt cgtcgcgcga catcccgtgg cgctcgccgc
     3481 gcagcacgag ctcgacccgc tcgggcgcgg gcgcccacac cctgaactcc atcagacgga
     3541 cccaccttct gttctggtag cgacgaggac ggggaagccg ccgagcagct cctccaacgg
     3601 cacgaccccc gagaacgtcg tgcccgtcaa cgtgtcggtc caatccccct cgggcagcgc
     3661 gaggtgcgtg tcgcgccacg ccccctccag tgtcagcacc aaccgcggcg cgacggtcag
     3721 gacccgcccg gccctcgtga acgcgacgac atggcgcgcc ctcggcccgg tcgcccacag
     3781 cgcctcgtag gccgagccct cgccgaacgc agccggatcc cgctcccgca gcgccagcac
     3841 acgttgcgtc aggagcagct tcgggagccc ctcgtccatg cgcgcgagcg cgcccgccgc
     3901 atcgccggcg agcacctctt cgaggagccg cgccctgacc tcgtagtcga ccgggcgccg
     3961 gttgtccggg tcgacgaggc taaggtccca cagctcgctg ccctggtaga cgtcggggac
     4021 gccgggatag gtgagcctta tcgccgtctg cgcgagcgag ttgatccgcc cgggctcgac
     4081 cagcggtgcg gcgaagtcct ctagctccgc cacgaactcc cggtcgtcga gcaccgctcg
     4141 gacgaacgcg gccaggccct cgtcgtactc cggcgacggg tccgtccacg cggtgtgcgc
     4201 cttcgcctcc ttcgacgcct tctccatgta cgcgacggcg cggtcggccg gcagcggcca
     4261 cgccccgacg agcgtctggt acagcaggta ctcggcgttg cggtccggga tgccgcccga
     4321 ccggtggcgg tcgttgtgtg ccttccagcg cgtgaccgcg tcgccccact gggccgggat
     4381 ctcggaaagg aggtcgatcc gcgcccgcac gtcctcggaa cgcttcgtgt cgtgcgtcga
     4441 ggtcgcgagc atcgcgagcg gccaccgctc ctgcgcccgg acagcgcggt cgtggaacgc
     4501 ctccagcgag acgccgaagc agccgaggtc acttcccacc tcgttcagcg agaccagccg
     4561 gttgaagttg tagaacaccg tgtcctcgac gcccttggcc atgaccggcc cggtcgtctg
     4621 ctggaagcgc agcacgaacg cgtcctcggc cgggcctgaa tatcgcatca gcaggacgtt
     4681 gcagaggaac tcgaacagct ccggcgcgag gtccggccgc cgcgctgccg cctcttcgac
     4741 cgcctcctgc acgtgcgcga cgtcctgcgg gctcggagtc ctcgtgcgcg tgtcgaggta
     4801 cgtccggtag acggggaacg cggcgatcgt ctcgcggatc gcctcgcgca gctcgtggcg
     4861 cgtgtagtcg cggtagcggc gatggctctc ggacacctcg acgaacaacg cagtcagccg
     4921 ttcgatgtcg ctgacgagca cctcgcgcat gatgaaaagc ttcttcgccc ggatcatctc
     4981 ggcgaggtcc gccggctcgc gcgtgaagtc gaggtacacg tccgacatcg ccttctccgc
     5041 gttcgagtcc acgaacagcc cgttgagcac gttgaggaag tcgtagcccg tcgtgccctc
     5101 gaccggccac gtctcgggga gctcctcgcc cgcctccagg atcttctcga cgaccaggta
     5161 cgcctcgggc gagcggctcc tgagcttctc gaggtacgcg ccggggtccc ggaggccgtc
     5221 cacgtggtcg atgcggaggc cgtcgaggtt gccgccctcg atcatcgaaa gcacgaggcg
     5281 gtgcgtgtcc tcgaacacgc gctcgtcttc tattcgcagc gcggcgagcg tcttgatgtc
     5341 gaagaaccgc cggtagttga ggtcgcggtc gcccgacttc cagaacgcga cgcggtagtg
     5401 ctgcttgtcc aggacctcgt gcagtgcgtc ggggtcggcg ttcacggcgt cgaggtcgtc
     5461 gggggcggtc ccgggcgaga ccggcagcac gtgctcgtgg tagcggagca cgacctctcc
     5521 gtcctcggcc gcgagccgca gctccccggc ttcgagcacc cggccgtagt ggtcgccgag
     5581 gatcggcacg aggatcgtgc ggtggagcat cgcctccggc gggtcccagt cgatgtcgaa
     5641 gtacccggcg taccggctcg acgcgccgtg cttgagaacg tcccaccacc acgcgttgct
     5701 cgcgtcggag atgcacatgt ggttcgggac cacgtcgagg acgtgtccca ggccgttcgc
     5761 cgagagggcc gcgcagaagc gctcgtgcgc gtccggcccc cccagctcgt cgttcacctt
     5821 cgagtggtcg gtgacgtcgt agccgtgcgc gctgccggcg gcggcctgga ggtacggcga
     5881 gcagtacgcg tgactgacgc cgagccgcgc gaggtagccg gtgacgccgg cggcatcgtc
     5941 gaaggtgaag cccgcgttga gctgcaggcg gtacgtggcc cggacgtcct tcggcacgct
     6001 aggcgctctc gtcgaccctc tgcaggatca cgagcgagcg tcccgtgacg gggagcttcg
     6061 cgccggcctc gtgcgtcgcc tctgcggggg tgccgagcgc ctcggcggtg tcgagctcgg
     6121 cggtccagcg cggcccccac gcctccgacg ggagcgtgaa ctcgatcgtg tcgtggtgcg
     6181 cgttgaacag cagcaggaag ctgtcgtcgg tgatgcgctc gcccttcggc ccccgctgcg
     6241 ggatctcgtc tccattgagg aagaccgcta tcgacttcgc gaagcccgcc gtccactcgt
     6301 cgtcgcccat ctcctcgccg gtgggggaga accagccgat atcgcgcacg ccggagccgt
     6361 ggatctcccg gcccatgaac cacttccgtc gccggaatat cgggtggcgc cgacggaagt
     6421 ccatcagccg ccgcgtgaac ccgagcagcg cgaggttctc gtcgcgcaac gcccagtcga
     6481 accacgagat ctcgttgtcc tggcagtagg cgttgttgtt cccgcgctga gtgcgtccga
     6541 tctcgtcgcc gccgagcagc atcggcactc cctgcgacag catcagcgtc gcgaggaagt
     6601 tgcgcttctg acgctcgcgc aggctgcgga tcgcggggtc gtcggtctcg ccctcctcgc
     6661 cgtggttcca cgaacggttg tggttgtcgc cgtcgcggtt gccctcgccg ttgtcctcgt
     6721 tgtgcttgtc gttgtacgag acgaggtcgt gcagcgtgaa cccgtcgtgc gccgtgatga
     6781 agttcacgct cgcgttcggg tggcgtccgt cgtcctggta gaggtccgag ctcccggtga
     6841 agcgcgaggc gaactcgccg agcctgctgt cctcaccgcg ccagaagtcg cgcacggtgt
     6901 cgcggtactt cccgttccac tccgaccaca aggcggggaa gttgccgacc tggtagccgc
     6961 cctcgcccac gtcccacggc tccgcgatca gcttcgtccg ggacagcacg gggtcctgat
     7021 ggatgatgtc gaagaacgcc gacagccggt cgacctcgtg gaactggcgg gcgagcgtcg
     7081 cggcgaggtc gaagcggaag ccgtcgacgt gcatctcggt agcccagtag cgcaggctgt
     7141 ccgtgatgac cttgagcggc tccgggtgcc ggacgttcag cgagttcccg gtccccgtcg
     7201 tgtcgaagta gaagcgccgg tcgtccggga cgagccggta gtagacggag ttgtcgacgc
     7261 ccttgaacga cagcgtgggg ccgaggtggt tgccctcggc cgtgtggttg tagacgacgt
     7321 cgaggatcac ctcgatcccg gcttcgtgca gcgacttcac catctgcttg aactccgtca
     7381 cctgctcgcc gcgcgtgccc gaggagctgt agccggagta gggcgcgagg tagccgatcg
     7441 agtcgtagcc ccagtagttg cgcagcccct tgtcgatcag catctgcggg tggacgaagt
     7501 ggtggacggg gagcagctcg accgcggtga cgccgaggag ctgcaggtgc tcgatcgcgg
     7561 cggggtgcgc caggcccgcg taggtcccgc ggatctcggg cgggatgccc tcgtgcgtga
     7621 tcgtgaagcc cttcacgtgc gtctcgtaga tcacggactc gtgccacggc gtgttcgggt
     7681 gcttgtcgtc gccccagtag aacgagccgt cgaccacgac cgacttcggc acgcgagcgc
     7741 cgtcgtcacg ctcgtcgaac gagaggtcct ccgcggagtc gcccatcttg taggcgaaca
     7801 cctccggccc ccagtccacg tcgccgtcga tcgcgagcgc gtaggggtcg agcaggagct
     7861 tggtcgggtt gaatcggagg ccgtcctggg gcttccaggg cccgtggacc cggtagccgt
     7921 agcgctggcc gggccctacg cccgggacgt aggcgtgcca gtaatgggcg gtgctctccg
     7981 tgacgccgag gcgcgtctcg gcccccgagt cgtcgaacaa gcagagctcg acggcatctg
     8041 cgttctccga atagagcgag aagttggagc cgcctccgtc ccaggtagcc ccaggtggga
     8101 agggcccccc gggccaggtc tccatagttc gagaagatat gccccgggcg gcagaggccg
     8161 aaccacgggg gccggtcggc cacaccccct cttccgagag gtgtcagaca tgacagacat
     8221 gactgccgtg tctgccgtgt ctgacacctc cggcgagagg cctacagcac gcgcagggct
     8281 ccggcctcgt ctcgctcccg acgcgtcagc gccctcagca ccgccaggtc cccgtgcttc
     8341 tgcctcgcca cgtccaccag cgcgtcgatc gccagcttcg tggcgtccgg cggcagcggc
     8401 ggggcctcga cgccgacgct caccgacagg tcgtcgacgt gcactacgag ctcgatcagt
     8461 cgcgtcacca ggtattcgtc gatcgtcagg acgatgccct tgtaggcctc gaccttgcgg
     8521 tccggcggct cggcttccag ccgggtccgc agcgtctcga gcagctcgta cgaacggtcc
     8581 cgcaccgacg cgaagccgcc cgcggcctcg tcttcgcccc gctgccggat cgcccggtgg
     8641 atctccgacg tgatgtcggg ctcgtcgacc gcctgcgcgt agtactcggc ggcgctcacg
     8701 ggttcacccg acggctcggg gcggtccaga taagcggcca ccgagccggt cgctcgcaac
     8761 aggtgcccgg cgaggccccg cacggtgaac tcgggcaacg cgctcggcga gtcccacgcg
     8821 gcctcgacct cgggccgggc cagcaggtcc gcggccaccc ggcccgtctc gaggaacgtc
     8881 gcccggacgt tcacgcgtcg tgtccccgta gtagccgcca ctgcgccttc gcctcgcgcg
     8941 atgtccactc ccacgctctc cgcggaagcg agtggagggc cgggaacaag agccccgcca
     9001 aacctagcga caacgccatg atcagaggat cgtacgtgcg cggccacgac tgttccgcga
     9061 cgagctgtcc cggcgagcgg tcgcgcaacc acgcgttgtg cagcccgtcg ggatggatcg
     9121 cgagcatcca cgccgccagc acctgatacg ggaggaagag cgggctgggg aacgccgcga
     9181 gggccgggac gacgatcgcc aggtactggt tcgcgatcga cggcgcgaac acgacgatcg
     9241 tgaccagata gaggagcgcg aggtccaacg gctcctcccg ccgccacacg aacccgagga
     9301 cgaggacgac cactccgaac agcacgatcg gcgagatcag cggctgcagc tgagccggca
     9361 gcacgccgtg ccagaacggc gcgttgtcgt gcccccggta gagggcgacg ttcgagacga
     9421 tcccgctccg tcccccggcg aggaacggga cgaagctgac ggcgaggacg gaaagcggca
     9481 ccgcgacggc gagcagcttc cgccgcagcc ccttctcgcg gatcgcgagc cagagcggga
     9541 ggaccagcag gacgtgcttg accgtcagcg agagcccgag caacaacagc gcgggcaaga
     9601 acgacgccgc gtacacgacc atcgcgagca accccaccag gaccgccagg ttgtcgaact
     9661 ggttgtggta gccggtgatg atgatcgaga tcgggttgag gaagaacagg aacccggcga
     9721 tgcggccgta gcgccggtgc agcaccgccc agatcccgac gtcggcgagc gtcagcacgg
     9781 cgatgatcag gatccggaag gcccccaccg gatccgagaa cagcccgccg aggtcgtgca
     9841 gcacgccgag cacgaagaac cagatcggcc cgtagttgta ccgctcggtc tcggcgtaga
     9901 cgttgccgcc gcggtcgacg atctccgcga cgatgccgta cgactcgagg tcgaagttgc
     9961 tgccgagcgt cgacacccag aagcgcagcg cgacgccgat cgcgaggatg agcacgatcg
    10021 ccagatcgcg ccgcgcgaac gagagcctgt agccggtcac cgcttgcttg tattcgccaa
    10081 gccgagcccg cgcaaacggc cgaagatcag atggttcccg ccgagatgcg gcgcgcgagg
    10141 tctcgcccca tcgcgatcgc gtagctctgc ccccggtcct gcgggtagac ctgcgccatg
    10201 ctcgccaggt agacgccttc gaccggggtc gtgtgcggcg ggatcgtgga gcggtagccg
    10261 acggtcacga ccggctgcgc gaacggggcg cgggagaagt gccagtcgag cacgtcgtcg
    10321 agtccgaacc cggggctcag ccgctgcagg tacggcaggt actcgtcgag gagcttctcc
    10381 ggctcggcgg tgtagcgcgg gtcgtcccgg gccacgtagt tgccgacgta gacgacgtgc
    10441 cgcccgccgt aggtggccgg gtcctccatg cgggtgtgct cgacgaccgc gaggaacggg
    10501 aacgacgggt cgttgacgtt cagccagtag tacgggacga ggctgcggcg gagctccagc
    10561 aggaagcacg tggcaccgag gtactccggc gagcgtcccg ccggcggcgc tccgatagcc
    10621 cgggcgagct cgaccggcgg cgtcgtgacg atcacgcgat cgaactcctc ggcggtcccg
    10681 ttggaggtag cgacgcgcac cgcgccggac gacctctcga tccgctcgac ggccttctcg
    10741 aaccggatga cgccgccgcg gttctcgatc gcagcggcga gcgcggcgta gagacggtgg
    10801 aagcccccgt gcagatagcc gagcctgaac gtgcggtggt ggatgcgcgc ccacagccac
    10861 gccatcgaga tctccggcgc gcgctccccg aacttcccgc gcagcagcgg ctcccagacg
    10921 acctcggtcg cccgccggcc cgcgtgcttc gacgtccacg cgagtgcctc gacgccttcg
    10981 agcttctcgt agcgcttcag cgccttgagg tacgcggcgc tggcggcgaa ccggacgcgg
    11041 tcgagggggc cgagcggcgg gaacgccagc atctcgcgcg gcgtcccgaa cgggtgcagg
    11101 cggccgtcgt agtagatgcc ggtcgtcggc gatcggaacc gcagggaatc tccgaggccc
    11161 agctcctcga cgaggccgac gagcgccttg tcgctcgcga agacgtggtg gtagtagcgc
    11221 tcgaccggag tgccgccgac ctcgatcgac gcgaccagcc cccccagctc cgggcccttc
    11281 tcgagcactg tcacgtcgtg cccggcgttc gctaacgtgt atccggccga cagccccgtc
    11341 gcgccggcac cgacgaggcc gaccctcaaa gcgaggggat cacggcagcg acgcggtgga
    11401 gtccagccgg ccttcccaca tgacgtcgcc gttcatggac tcgtcgccgt cctccggctc
    11461 gagcgtgatc tgcaggtacg ggtagtccgc ggggtccacg ccgacgggga agtggacgcc
    11521 ccccggatcg ccggcgacga acgtcccgca cgagacgcgc cccgacgccc cgaccatcca
    11581 gagctcgtag tagtagccgt cgggggccgg cgggatcggg ccgtccatct cgacgcggta
    11641 gccggcgccg ccgtagcgga cgagctcgac gtcctcgggc ggcaccgagt ccccctggag
    11701 ctcgacgagc tgcatcgtct ggcctgccgg gccgaggtcc tcgcgcatgc ggtcggcctg
    11761 cgatcgccag ttgatcccga gcacgccgag cacgacggtc gcgacgccga gagcggggac
    11821 gaggacggtc gtagcgcgcg accgcctcgg ggtcccgatc ccggcccgct cgagcgctgc
    11881 acgctcgagg ccgtcgggag gttcgacgga gagcggcacc tgggacgcga gcctgccgac
    11941 gcggtcgagc tcgacgaacg cgaacacgtc ttcttccagg agcggccccg gcgcgtgctc
    12001 cagcggagcg tcgacgagca gcttgtgcac cccggcgacc cgtcccagct cgccgcggca
    12061 ctcgtcgcac ccgtccacgt gtcgccggac ctcctgcgcg gccggcggtg cgagctcgcc
    12121 catcaggtac gagtggaagt cgagcctgac gtcgtcgcag cgcgtcacgc tgccccctcg
    12181 ggcgcgatcc cgcgctcgac gaagatcgcc cggagcttgc gcagccccgc gtacaggcgc
    12241 gtcttgacgg tcccgagcgg tatccccagc tcggccgcta tctcgctctg agtcatctgg
    12301 ttcgcatacg caagctcgac cacacggcgt tgatcttccg gcagacgggc gaccgcgcgc
    12361 gccacctccc atgccgtcca agcccggtcc tcgtcccacg gcgccgttcc ctcggggggc
    12421 tcggccaccg gcatgggtgc cttcgagcgg gcgcgggcga ggtccgcggc cacgttgcgg
    12481 gcgatcccga agatccacga gctggacgcc ccgcgctcgg ggtcgtaccg gcccgccctt
    12541 ctccacacgc gcaacgtcac ctcctgtacc agctcctccg ccagcttcgg gtcgttcagc
    12601 cacctgatcc cgagcgcgta cagcggccgt tcgaacgacc ggtacagcgc ccgcagcgcg
    12661 tcgacgtctc cgcgagcgac gctctggagc agctggtcct cgtcgacccg aagcgcttga
    12721 cctccgggga ctccagtcgc cattacccct ccgggatcgt gaaaggttct ccctcagtct
    12781 cgcagagggc cggtgttact ctgcgggcga tgccgaccgc cgatcccgcg gcgctccgtc
    12841 gtcttctgga gctccagaac gaggacaccg cgatcaaacg actcgaagag cggcgcgcat
    12901 cgcttcccga ggcccagcgg ctcgccgagg tgaacgccgc cctcgccgag ctcgacgcgg
    12961 acctcgcgat cgcgcgcaag cagcacgacg aggtcgcgcg cgaggtcgcg cggctagagg
    13021 gcgaggccgg cctcctcgac cagaagatcg cccgcgagga gcagcggatg ttctccggcg
    13081 gcgtctcgaa cccgaaggag ctgtccgctc tccaggccga ggtcgagtcg ctcaagcgca
    13141 ggaagtcggg gctcgaggac gagctgctcg aggcgatgct cggccgcgag caggcgactg
    13201 cgacgatcga cggcctcacg aaggagcggg acgaggcggc gaccgaggcg gcggacctga
    13261 acggcgtcgt cgaagggatc accggcgaga tctccggcga gctcgagacg cacacgacgg
    13321 cccgcacggc gatctccggg gagatcccgg cggaccttct gaagctctac gagcagctgc
    13381 gcgcgcagaa gggcggcgtg ggggccgcgg gcctcgaagg cgggacgtgc ctcggatgcc
    13441 acacgaagct cccgcagcgc gagctcgaac ggatgcgggc cgaaggcggt ctccagcgct
    13501 gcgacaactg ccgccgaatc ctcgtcatcg actagcgatg gcctcggcca tcgcgccaga
    13561 aggaagctga cgcatgaggg ggttcctcca cgccgacggc ggagcacggg gtaaccccgg
    13621 c
//
LOCUS       DASQDO010000335         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_648379, whole genome shotgun sequence.
ACCESSION   DASQDO010000335 DASQDO010000000
VERSION     DASQDO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648379"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1160
                     /locus_tag="VEV43_09455"
     CDS             219..1160
                     /locus_tag="VEV43_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP23788.1"
                     /translation="MAVVEAVGLVKTFRTGRGRSARTVEAVAGIGFSVEAGERLAYIG
                     PNGAGKSTSIKILTGILHPDEGSAQVLGLVPWEQRRRLALEIGTLFGQRSQLWLELPA
                     RESLSLLGAIYRLGKDRTERRIAELGELLEASDLFDTPVRHLSLGQRMRCELAASLLH
                     EPQVLFLDEPTIGLDLVAKQRFRDLVVRLNEETGTTLFLTSHDVSDIEQVARRVIVIN
                     HGRLIYDDKVSVMRRSLLRTKLLEVRFDDRVDDLAVEGAEVVKGSGTGYKLRVDTDRR
                     PIRHVLDDLLDRYHVADISVLDPPLEDVITEIYEARP"
     gene            1157..1933
                     /locus_tag="VEV43_09460"
     CDS             1157..1933
                     /locus_tag="VEV43_09460"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HYP23789.1"
                     /translation="MSAYAAGAVAGFRRLVASPGEMVARACFYGVIVGVLSSLWRAAA
                     DATGGSIVGYDVAAITWYIVFSEASVNGTKFRMIEHIGTEIGSGAFAVEMLRPVRAVT
                     FRMAGELGDGVVRLGAMVGVGTLFGTLIAGPPPRLETYVLAIVSALLAISCNIAAQHA
                     FAAMAFWQHEAKGAWFLYQKLVFVLGGMLLPLQIFPAWLESAAWASPFWTMSYAPARL
                     AAGFAEPWLLLGQAAWLVVLYGAAVAAFAAGERHLQEVGA"
     gene            1930..2697
                     /locus_tag="VEV43_09465"
     CDS             1930..2697
                     /locus_tag="VEV43_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HYP23790.1"
                     /translation="MIFAATMRKAFSEAWAKRSSFWFQVGVMVANDLFFVAFWFLFFN
                     EVESVRGWDVERTLLLLAMLATVSGAALGLFANARWLGQTISDGGMDAALALPANPLT
                     YLLGRSVDPSMLGDLVFGPLLFVALGDLSVERVALFVLGSAVGTVVFVSFLVLLASLT
                     FFVGGRGEHAELGFQAILMLSSYPPDIFAGATKLVLFTVVPAAFVSGLPTRLVDEFSP
                     ALASSMVGVAALFAVGAYAAFGAGVRRYRSGSAWTGA"
     gene            complement(2701..>3075)
                     /locus_tag="VEV43_09470"
     CDS             complement(2701..>3075)
                     /locus_tag="VEV43_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23791.1"
                     /translation="VPVEGGDPVRLTDDPVDDYAAEWSPDGSMLVFVRGRLEGNEPPD
                     EVFVMNADGSGQTSVSPAELQAFSPDWSPDGERIVFVGQVPANPRPPEWELFTVRPDG
                     SDLTRLTTSEPEEFDPEWGPQP"
BASE COUNT          381 a         1028 c         1127 g          539 t
ORIGIN      
        1 ggacgggggc cgccttcggg cggccccttc acgcgtccgc tggacgcgag acaaccgcct
       61 cgtctatctt ttccccatgc acctgttcgt cgcgaagcag aaccttatcg acgccccgac
      121 gtgggcgagg gggctgcgcg cgccgtaagc atgtgcactg cacgttacgg acgcagcccc
      181 gggtaaccgg ggcttttttg ttttccgggg agaacgcgat ggctgtcgtc gaagcggtcg
      241 gactcgtcaa gaccttccgg accggaaggg gcaggtccgc gcgcacggtg gaggccgtcg
      301 cgggcatcgg cttctcggtc gaggccggcg agcggctcgc ctacatcggg ccgaacggcg
      361 cgggcaagtc gacgtcgatc aagatcctga cggggatcct gcacccggac gagggcagcg
      421 ctcaggtgct cgggctcgtc ccgtgggagc aacggcgccg gctcgcgctc gagatcggga
      481 cgctgttcgg gcagcgttcg cagctgtggc tcgagctccc cgcgcgcgag tcgctgtcgc
      541 tgctcggcgc gatctaccgg ctcgggaagg accgtacgga gcgccgtatc gccgagctcg
      601 gcgagctgct ggaggcgtcg gatctgttcg acacgcccgt gcggcacctg tcgctcggac
      661 agcggatgcg gtgcgagctg gccgcgagcc tgctgcacga gccgcaggtg ctgttcctcg
      721 acgagccgac gatcgggctc gacctcgtcg cgaagcagcg cttccgcgac ctcgtcgtgc
      781 gcctgaacga ggagaccggg acgactttgt tcctcacgtc gcacgacgtc tccgacatcg
      841 agcaggtcgc gcgccgggtg atcgtcatca accacggccg gctcatctac gacgacaagg
      901 tctccgtgat gaggcggagc ctgctgcgca cgaagctcct cgaggtgagg ttcgacgacc
      961 gcgtcgacga cctcgcggtc gagggcgcgg aggtcgtcaa gggatcgggc acgggataca
     1021 agctgcgcgt cgacaccgac cggcggccga tcaggcacgt gctcgacgac ctgctcgacc
     1081 gctatcacgt cgccgacatc tcggtcctcg acccgccgct cgaggacgtg atcaccgaga
     1141 tctacgaggc gcggccgtga gcgcctacgc cgcgggggcc gtcgccgggt tccgccggct
     1201 cgtcgcgtcg ccgggcgaga tggtcgcgcg cgcctgcttc tacggcgtga tcgtcggggt
     1261 gctgtcgtcg ctgtggcgtg ctgcggcaga cgcgaccggc ggctcgatcg tcggatacga
     1321 cgtggcggcg atcacctggt acatcgtgtt ctcagaggcg tcggtgaacg ggacgaagtt
     1381 ccggatgatc gagcacatcg gcaccgagat cgggtccggc gccttcgccg tcgagatgct
     1441 gcggccggtg cgcgccgtga cgttccggat ggcgggcgag ctcggcgacg gcgtcgtgag
     1501 gctgggcgcg atggtcgggg tgggaacgct gttcgggacg ctgatcgcgg gccctccccc
     1561 gcggctcgag acctacgtcc ttgcgatcgt ctcggcgctg ctcgcgatct cctgcaacat
     1621 cgcggcgcag cacgcgttcg cggcaatggc gttctggcag cacgaggcga agggcgcgtg
     1681 gttcctgtac cagaagctcg tgttcgtgct cggcgggatg ctgctgccct tgcagatctt
     1741 ccccgcgtgg ctcgagtccg cggcgtgggc gtcaccgttc tggacgatgt cgtacgcgcc
     1801 ggcccgattg gctgcgggct tcgccgagcc gtggctgctg ctcgggcagg ccgcgtggct
     1861 ggtcgtgctg tacggcgcgg ccgtcgccgc gttcgccgcg ggtgagcggc acctccagga
     1921 ggtcggcgca tgatcttcgc cgcgacgatg cgcaaggcgt tctcggaggc gtgggcgaag
     1981 cgatcgtcgt tctggttcca agtcggggtg atggtcgcga acgacctgtt cttcgtcgcg
     2041 ttctggttcc tgttcttcaa cgaggtcgag agcgtgcgcg ggtgggacgt cgagcgcacc
     2101 ctgctgctgc tcgcgatgct ggcgaccgtc tcgggggccg cgctgggcct gttcgcgaac
     2161 gcgcgctggc tcggccagac gatctccgac ggagggatgg acgcggccct cgcgcttccc
     2221 gcgaacccgc tgacctatct gctcggccgg tcggtcgacc cgtcgatgct gggtgacctc
     2281 gtcttcggcc ccctgctgtt cgtcgccctc ggggacctgt cggtcgagag ggtcgcgctg
     2341 ttcgtgctcg gctcggcggt cgggacggtg gtgttcgtct ccttcctggt gctgctcgcg
     2401 tcgctgacgt tcttcgtggg gggccgcggc gagcacgcgg agctggggtt ccaggcgatc
     2461 ctgatgctgt cgtcgtaccc gccggacatc ttcgcggggg cgacgaagct ggtcctgttc
     2521 acggtggtcc ccgcggcgtt cgtgtccggc ctgccgacgc gccttgtcga cgagttctcg
     2581 ccggccctgg cgagctcgat ggtcggggtc gcggccctgt tcgcggtagg tgcgtacgcg
     2641 gccttcgggg ccggggtgcg tcgataccgc tcgggctccg cgtggacggg ggcctaggcg
     2701 ctagggctgc ggcccccatt cggggtcgaa ctcttccggc tccgacgtcg tcaagcgcgt
     2761 caggtccgag ccgtcgggcc gcaccgtgaa cagctcccat tccggcggcc gcgggttcgc
     2821 cgggacctgg cccacgaaca cgatccgctc cccgtccggc gaccagtccg gcgagaacgc
     2881 ctgcagctcc gccggcgaca cgctcgtctg cccggacccg tccgcgttca tcacgaagac
     2941 ctcgtcgggc ggctcgttgc cctcgaggcg gccgcgcacg aacacgagca tggacccgtc
     3001 gggcgaccac tcggccgcgt agtcgtcgac ggggtcgtcg gtcagacgga cggggtcacc
     3061 gccttcgacc ggcac
//
LOCUS       DASQDO010000336         6358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_892843, whole genome shotgun sequence.
ACCESSION   DASQDO010000336 DASQDO010000000
VERSION     DASQDO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892843"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..952
                     /locus_tag="VEV43_09475"
     CDS             146..952
                     /locus_tag="VEV43_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HYP23792.1"
                     /translation="MAIKHEIKGTVNQMAVCQLDPGQTMYCEAGKFLWKTANVGLETR
                     FTTPDQEEQNKGKGLLGKALSTAVEVGKRQLAGESLAFQYCTPTGGSGLVSFAGTLPG
                     EMREIALDGTTTWTAEKDAFVAAESTVKFDIQFAGFTTGRRGGEGFVLEKFTGTGSLF
                     IAGAGNFIDLNPADYGGKLQVDTGCVVAFDDGITYGVEHIGKLNAQGMKTAMFGGESF
                     SLATLEGNGTVILQSVSMVALARTLMGAATQASTEGTAGSIRGILGGGTD"
     gene            993..1307
                     /locus_tag="VEV43_09480"
     CDS             993..1307
                     /locus_tag="VEV43_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23793.1"
                     /translation="MDKMKAQAQNVAAQAKTAAGQAQDKMEQMQLRKKADEAAKQLGY
                     LIHKERTGGAAAGAEADKLGAEISDLERQMAEEDASQSGAPSAPATPTSSSDATPGDF
                     KL"
     gene            1414..2631
                     /locus_tag="VEV43_09485"
     CDS             1414..2631
                     /locus_tag="VEV43_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23794.1"
                     /translation="MEMRRSPALRADHSRLLLELSREIVSTLDPQEVLERGLAAFARI
                     LPFESALIRVLDEDSLLTAATTPAGSASLEVRIPAESGLAGAVMSSIEPIHIPVLGPQ
                     HAADPSVLALASGGELRSYLGVALADSEGFLGVLELGSTAERAFGPDGIASAVVLAPS
                     LAIGIRNSRVAAAGPVLDEDFMSLVAHELRTPLAVVLGCAETMASKARSLEPAVVEDL
                     SRRAVAAAHRLERLIADLFDMSRIRNGTLTVVPVPTSLRSIVSRAISDAPPGLSVVDD
                     LEEGLPLVDVDPNRLVQVLRILFDNAWKHAGEGAEVDVRACRRDGGVIVEIADRGPGI
                     DEAILHKVFDPYVQAEEPMTRRVGGLGVSLYLARGLCDLMGAGLDAHARSGGGTVFVL
                     RLKAATEELERVT"
     gene            2756..4882
                     /locus_tag="VEV43_09490"
     CDS             2756..4882
                     /locus_tag="VEV43_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HYP23795.1"
                     /translation="MGKIGNDVGKNDLGLRRDPAPQTIAVDLPPLVLGLGDKRALNPD
                     IAGAKAANLARAMQGGFTVLDGFVVTVEGTQVFSTQRARQTGRDLEKLLKKRWAALSA
                     EGTRPLVVRSSSSAEDGHDSAMAGMFTSVLDVVTWADFRRAMQTVVESARIIDLVDET
                     ETFAPMAVLVQPQLAPQWGGVLFGVDPVTGRADHLVVAAIEGRPDALVSGTVEGHRYV
                     LTHSGRVVERSDPGGLSKPVLKRLTKLAERAAGHFGGAQDVEWALLDDELIMLQSRPV
                     TAVADPRSAVGPVLGPGPVAETFPQPLSPLESDLWVEPLRTAIAEVLVLTGAASKKTL
                     SRSPVVTTVGGRVAADLKLLGAFPGKKTFFETIDPRPPSRRLAAAWRTGRLRAALPSI
                     AAAIVDKVDDDLAAVPELATLDNGELVEVLRRSRQALVALHAHEVMFGLLADEQYTVT
                     AASLGLRHLAGARTEGLADADIVAMRPEVLALVPPSIFGDGTLPETGSLLPRSQEDGA
                     LPQREALRLRARWVQELSALVVRLLADRLHEGGRVADRAAVGELTLDEVETAASLGAL
                     PSGICDRRHEPGPALPAAFRLTNAGDVVVFRPRKQKKSGSAGTGASAGRGEGIVHSGD
                     GVPEKGQVLVVSTLDPDLATLLPRLGGLVAETGSVLSHLAIMAREYGVPTVVGVPDAV
                     TRFPAGARVSVDGTTGEVTLVEEMTP"
     gene            4879..5754
                     /locus_tag="VEV43_09495"
     CDS             4879..5754
                     /locus_tag="VEV43_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23796.1"
                     /translation="MSSKRIAYAVLTVVLAASGTYLFVYLYRWEWNRTLIAGIFFVAA
                     EIALVGAAILERLKKMESRIESRVAEPPALAHIQETAPEPKAGFKWLTRTGGTNVFVP
                     VLMGAGFVVSGIAWLVERFAHATARPALERGLAWRLAPLSLPAGGFVGGVTVLAPRPS
                     RTKARVKHTLITIAVAAALSLTVDALGDLTQNRPDRALPGTSSTVVLEIHTRTADASS
                     VETATRLWAACAGTVPGYIEAPKIASLGSGKALIVTQPSLGEHTQRRLSGCLSDTTLD
                     EVVARVVSVESFGVE"
     gene            complement(5764..>6358)
                     /locus_tag="VEV43_09500"
     CDS             complement(5764..>6358)
                     /locus_tag="VEV43_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HYP23797.1"
                     /translation="FLALGNLAIFSAMVWAKTTAPAQNVPAPAGVMNFQVVDDTVWRG
                     SHPKEETFRELAKRGVTTIVDLRAEEWVDEDEKFLRRLGITRFNIPLRDGQVPTEAQV
                     QSFLNIIEHAPGKVYVHCMAGVGRTGAMVAAYLVATGQEDPAGALRRNLSVGPPSLEQ
                     LAFVADLTPTEADRPNAVVTGLSRVLDAPRRIWSNIN"
BASE COUNT          950 a         2192 c         2279 g          937 t
ORIGIN      
        1 catgacggtc ccgcctccga ccccgaggag cccgctcgcg acgcccgcgg cggctccgat
       61 tgcaatcagc ccaaccacct tgaccgcatc ttcgcaggct ctctagcgtg ggaagcgact
      121 cagctcatcc ccgaaaggcg acgtcgtggc gatcaagcac gagatcaagg gcacggtgaa
      181 ccagatggcc gtgtgccagc tggaccccgg tcagacgatg tactgcgaag caggcaagtt
      241 cctgtggaag acggcgaacg tcggtctcga gacccgcttc acgacacccg accaagaaga
      301 gcagaacaaa ggcaagggtc tcctcgggaa ggcgttgtcg accgcggtcg aggtcggcaa
      361 gcgccagctc gccggcgagt cgctcgcgtt ccagtactgc acgcccaccg gtgggtccgg
      421 tctcgtctcc ttcgcaggga cgcttccggg cgagatgcgc gagatcgcgc tcgacgggac
      481 gacgacgtgg acggccgaga aggacgcgtt cgtagcggcc gagagcacgg tgaagttcga
      541 catccagttc gcgggcttca cgacgggccg tcgcggcggc gagggcttcg tgctcgagaa
      601 gttcacgggg accgggtcgc tgttcatcgc gggggccggg aacttcatcg acctgaaccc
      661 cgccgactac ggcggcaagc tccaggtcga caccggctgc gtcgtcgcgt tcgacgacgg
      721 catcacatac ggcgtcgagc acatcggcaa gctcaacgcg cagggcatga agaccgcgat
      781 gttcggcggc gagagcttct cgctcgccac gctcgagggc aacgggaccg tgatcctcca
      841 gtcggtctcg atggtcgccc tggcccgtac cctgatgggg gccgcgacgc aggcttcgac
      901 ggaggggacg gccggttcga tcaggggcat cctgggcggc ggcacggact gacgatcccg
      961 aggacgatcc aaggaggacg acgatgggct tgatggacaa gatgaaggcg caggcgcaga
     1021 acgtggctgc gcaggcgaag accgccgccg ggcaggcgca ggacaagatg gagcagatgc
     1081 agctccgcaa gaaggccgac gaggccgcta agcagctcgg ctacctgatc cacaaggagc
     1141 gcaccggcgg ggccgccgcg ggggccgagg ccgacaagct cggcgccgag atctccgacc
     1201 tcgagcgcca gatggccgag gaggacgcct cgcagtcggg cgccccgtcg gcgccggcga
     1261 ccccgacgtc ttcgtccgac gcgaccccgg gggacttcaa gctctaaagc cccgcttttc
     1321 cgggcgtgcc ggacacgtcg gacatgcaat gacgaccgcc ggtacgacca aatacggatg
     1381 gacccgggga gtcgccgccg ccacgatttg gcgatggaga tgcgccggag cccggccctg
     1441 cgggccgacc actcgcgact gctcctggag ctgagccgag agatcgtctc gacgctcgac
     1501 ccccaggagg tgctcgagcg cgggctcgca gcgttcgcga ggatcctccc gttcgagagc
     1561 gcgttgatcc gcgtcctgga cgaggactcc ctgctgaccg cggcgacgac gcccgccggc
     1621 agcgcctcgc tcgaggtccg catacccgcg gagtcggggc tggccggcgc ggtcatgtcg
     1681 tccatcgagc ccatccacat cccggtgctc ggcccccagc acgcggccga cccgtcggtg
     1741 ctcgcgctgg cgtcgggcgg cgagctgcgc tcctacctgg gggttgcgct cgccgacagc
     1801 gagggattcc tcggcgtgct cgagctcggg tcgaccgcgg agcgcgcctt cgggccggac
     1861 gggatcgcgt ccgcggtcgt gctggccccg tcgcttgcga tcggcatccg gaactcgcgc
     1921 gtcgcagcgg ccggcccggt gctggacgag gacttcatgt cgctcgtcgc gcacgagctg
     1981 aggactcccc tcgcggtcgt cctcgggtgt gcggagacga tggcgtcgaa ggcccgcagc
     2041 ctcgagcctg cggtcgtgga agacctcagc cggcgcgcgg tggcggcggc tcatcgcctc
     2101 gagcgcctga tcgcggacct cttcgacatg tcgcgcatcc gcaacgggac tctgacggtc
     2161 gtgccggtgc cgacgtccct gcggagcatc gtctcgcggg cgatcagcga cgcgccgccg
     2221 ggcctgagcg tcgtcgacga cctcgaggag ggcctgccgt tggtcgacgt cgatccgaac
     2281 cgcctggtcc aggtgctgcg catcctgttc gacaacgcgt ggaagcacgc gggggagggc
     2341 gcggaggtcg acgtccgtgc ctgccgcagg gacgggggcg tgatcgtcga gatcgcggac
     2401 cgggggccgg ggatagacga agcgatcctc cacaaggtct tcgacccgta cgtgcaagcg
     2461 gaggaaccga tgacgcgacg ggtcggcggc ctcggcgtaa gcctctatct cgcgcgcggg
     2521 ctctgcgact tgatgggcgc cggcctcgac gcgcacgcgc gctccggtgg ggggaccgtg
     2581 ttcgtcctcc ggctgaaggc cgcgaccgaa gagctggagc gcgtcacgtg aaggccggca
     2641 atcgtccgtt actagtccac tcggctcatt tgtcggcaaa accggacatc gaccttgctt
     2701 cggtggaatc gagcgactta acgtgacctt cagagaaaca agcggagggc tgcacatggg
     2761 caagatcggg aacgacgtcg gcaagaacga cctcggactc cggcgcgatc ccgcccctca
     2821 gacgatcgcc gtcgacctcc ccccgttggt gctcggactc ggcgacaagc gcgcgctgaa
     2881 tccggacata gcgggagcaa aggccgcaaa cctcgcccgc gctatgcaag gcggcttcac
     2941 cgtcctcgac ggcttcgtcg tcacggtcga ggggacgcag gtcttctcga cacagcgcgc
     3001 gcgtcagacc ggacgcgacc tcgagaagct cctcaagaag aggtgggccg cgctgtcggc
     3061 cgaaggaacc cgtccgctcg tcgtccgctc gtcgtcgagc gccgaggacg ggcacgactc
     3121 cgcgatggcc ggcatgttca cgtccgtgct cgacgtcgtg acgtgggcgg acttccgcag
     3181 ggcgatgcag accgtcgtcg agtccgcgcg catcatcgac ctcgtcgacg agaccgagac
     3241 gttcgcgccg atggccgtcc tcgtgcagcc gcagctcgcg ccgcagtggg gcggggtgct
     3301 gttcggggtc gaccccgtga ccgggcgcgc cgaccatctc gtcgtcgctg cgatcgaggg
     3361 acgtcccgac gctctcgtca gcgggaccgt cgagggacac cgctacgtgc tcacgcactc
     3421 gggccgcgtc gtcgagcgat ccgatcccgg cggcctctcg aagccggtgc tgaaacgcct
     3481 gacgaagctc gcggagcgcg cggccggtca cttcggcggc gcgcaggacg tcgagtgggc
     3541 gctcctcgac gacgagctga tcatgctgca gagccggccc gtcaccgcgg tcgccgaccc
     3601 gcgcagcgcg gtggggccgg tgctcggccc gggaccggtc gccgagacgt tcccgcagcc
     3661 gctgagcccg ctcgagtccg acctgtgggt cgagccgttg cgcaccgcca tcgccgaggt
     3721 cctggtgctg acgggggctg cttcgaagaa gacgctctcg cgctcgccgg tcgtgaccac
     3781 cgttgggggc cgcgtcgccg cggacctgaa gctcctcggt gcgttccccg gcaagaagac
     3841 gttcttcgag acgatcgacc cgcggccccc gtccaggcgg ctggccgccg cgtggaggac
     3901 cggccggctt cgcgccgcgc tcccctccat cgccgccgcg atcgtcgaca aggtcgacga
     3961 cgacctcgcc gcggtccccg agctcgcgac gctcgacaac ggtgagctcg tcgaggtgct
     4021 gcgacggtcg cgccaggcac tcgtcgcgct gcacgcgcac gaggtgatgt tcggtctcct
     4081 cgccgacgag cagtacacgg tgaccgccgc ttcgctgggg ctgaggcatc tcgccggcgc
     4141 gcgcacggaa gggctcgccg acgccgacat cgtcgcgatg cggcccgagg tcctcgcgct
     4201 ggtcccgccc tcgatcttcg gcgacggcac gcttcccgag accgggagcc tgctgccgcg
     4261 ctcgcaggaa gacggggcgc tccctcagcg tgaggcgctg cgtctgcgcg ctcgatgggt
     4321 ccaggagctg tcggccctcg tcgttcgtct gctcgccgat cgtctgcacg aagggggccg
     4381 cgtcgccgac cgggcggccg tcggcgagct gacgctcgac gaggtcgaga ccgccgcgtc
     4441 gctcggtgcg cttccgtccg gcatctgcga ccgcagacac gagcccgggc ccgcgctgcc
     4501 ggccgccttc cgtctgacga acgccggcga cgtcgtcgtg ttccggccgc gcaagcagaa
     4561 gaagtccggc tcggccggta ccggcgcgag cgccgggcgc ggcgagggca tcgtccacag
     4621 cggcgacggc gtacccgaga aaggacaggt gctcgtcgtg tcgacgctcg acccggacct
     4681 cgcgacgctc ctgccgcggc tcggcgggct cgtggccgag accgggagcg tgctctccca
     4741 cctcgcgatc atggcgcgcg agtacggggt tccgaccgtc gtcggcgtcc ccgacgcggt
     4801 gactcggttc cccgcgggtg cgcgcgtgtc ggtcgacggc acgaccggcg aggtcacgct
     4861 ggtcgaggag atgacgccgt gagctccaag cgcatcgcct acgcagtcct caccgtcgtg
     4921 ctggccgcct ccggcactta cttgttcgtg tacctgtacc ggtgggagtg gaaccgcacg
     4981 ctgatcgccg ggatcttctt cgtcgccgcc gagatcgcgc tcgtcggcgc ggcgatcctc
     5041 gaacggctga agaagatgga gagccggata gagagccgcg tcgccgagcc ccccgctctc
     5101 gctcacatcc aggagaccgc ccccgagccg aaggcagggt tcaagtggct gacgcgcacg
     5161 ggcgggacca acgtgttcgt gccggtgctc atgggggccg ggttcgtggt gtcggggatc
     5221 gcgtggctcg tcgaacggtt tgcgcacgcg accgcccggc ctgcgctcga gcgcggcctc
     5281 gcgtggcggc tcgcgccgct gtcgcttccg gccggcggct tcgtcggcgg cgtcacggtg
     5341 ctcgcgccgc gtccgtcgcg cacgaaggcg cgcgtcaagc acacgctgat cacgatcgcc
     5401 gtcgcggcag cgctgtcgtt gaccgtcgac gcgctcggcg acctgaccca gaaccgcccc
     5461 gaccgcgcgc tgccgggcac gagcagcacg gtggtcctcg agatccacac gcgcaccgct
     5521 gacgcgtcgt cggtcgagac cgcgacgcgg ctgtgggccg cttgcgcggg aacggttccc
     5581 ggctacatcg aggcgccgaa gatcgcgtca ctcgggagcg gcaaggcttt gatcgtgacg
     5641 cagccgtcgc tcggcgagca cacgcagagg cgcctctcgg gatgcctgag cgacacgacc
     5701 ctcgacgagg tcgtggcgcg cgtggtgagc gtggagagct tcggggtcga gtagccgttc
     5761 cggctagttg atgttcgacc agatccggcg gggggcgtcg agcacccggc tcagtccggt
     5821 cacgaccgcg ttcggacgat cggcctcggt aggcgtcagg tcggcgacga aggcgagctg
     5881 ctcgagcgac ggcggaccga ccgacaggtt gcgccgcaag gctccggccg ggtcttcctg
     5941 accggtggcg acgaggtagg ccgcgaccat cgctcccgtg cggccgacgc ccgccatgca
     6001 gtgcacgtag accttgccgg gggcgtgctc gatgatgttc aggaagctct ggacctgcgc
     6061 ctcggtcggg acctggccgt cgcgcagcgg gatgttgaag cgcgtgatgc cgaggcggcg
     6121 caggaatttc tcgtcctcgt cgacccactc ttcggcacgg aggtcgacga tcgtcgtgac
     6181 gccgcgcttc gcaagctcgc ggaacgtctc ctccttcggg tgcgagcctc gccagacggt
     6241 gtcatcgacg acctggaagt tcatgacgcc tgcgggggcc gggacgttct gtgccggcgc
     6301 ggtcgtcttc gcccagacca tcgccgagaa gatggcgagg ttgccgaggg cgaggaag
//
LOCUS       DASQDO010000337         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_403766, whole genome shotgun sequence.
ACCESSION   DASQDO010000337 DASQDO010000000
VERSION     DASQDO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403766"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VEV43_09505"
     CDS             <1..438
                     /locus_tag="VEV43_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23798.1"
                     /translation="DQFDEPNDITVFVIHHHLVPIPGTGRERNIILDAGDVLAELTQL
                     DIDVALSGHKHVPYFWGLNGMLICNSSTATTKRLRGLTPPSWNELQIDASTIKCYTHY
                     RDRRELSLIFSLRTRTMTREAFYLTEKFLESNQLLMDQRSVVG"
     gene            534..1433
                     /locus_tag="VEV43_09510"
     CDS             534..1433
                     /locus_tag="VEV43_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HYP23799.1"
                     /translation="MPENRPRIRLALFGALLALPLVAVPTNVLSATAFCDPADDPHGV
                     TSAARGSSTAREPEGMTGEEVPPTHKRPKKFSATIPVYFHVISAGTTPEAGNVTDAQI
                     AAQMTALNKSFGGSYGGFKTPFTFRLEGVTRTVNAEWFNMGYGSKAERDAKAALRRGG
                     ANALNVYSTAGGGLLGWATFPSSYREHPERDGIVVHAGSLPGGHVTRFNLGFTATHEA
                     GHWLGLFHTFQGGCNANGDYVADTPAQRIATSGCPEGQDSCSEPGFDPIHNFMDYSDD
                     ACYTQFTSGQSARMVDQFTYYRS"
     gene            1477..2643
                     /locus_tag="VEV43_09515"
     CDS             1477..2643
                     /locus_tag="VEV43_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYP23800.1"
                     /translation="MRTSLRIALPLLVVASLVLPAAPAAVAQDAPGVTLHAGRKIMEF
                     GQSVILRGAVDPPAEGETVSIVDDTGHEWATTTTDEKGRFHVKVAPRRTRQFHAVWVA
                     VPSEPVTVKVRPRVNVSLRAVRLFGKARIVGTVAPVQESGRVAVTLRRDGKSAWTRRV
                     ELRKGRKFSTTFEIGKIGRFAAIASFTDEAGVRGGEWSDAKSTPLPYLSVGARGVYVR
                     LLEQRLCELRYKLLGCNRRFGIDTADAMRAFNKIEGRARLGTVDGPTWAALARANVAK
                     PRHGGKGFHIEIDQTKQVVLMVEDGKVTHVLHTSTGAGGATRDGTFHVYRKLEGYSPN
                     RLYYPSYFDGLRAFHGWPEVPTYNASHGCARVPMWSATWVYGKADIGTTVYVYH"
     gene            complement(2757..>3693)
                     /locus_tag="VEV43_09520"
     CDS             complement(2757..>3693)
                     /locus_tag="VEV43_09520"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP23801.1"
                     /translation="KAAHMAGFEYMTHDDPAPPVGRTWDQRIRDCGYSSGAGENIAYG
                     YRTPQAVVEGWLDSAGHRRNIENPSYKVIGVGAAVNADGTPYWAQIFGLRVEAGDVAQ
                     VPSPAPVPPPPLPSPTPTPTPTPTPTPPSVPGAPLVADDSVTVPEDTSARITPLSNDS
                     GIGDLLAVGEPAHGTVSLGSGDEVVYVPDPDFHGSDSFTYSAFGASAPGRVTVTVEPR
                     NDAPRAGDDSAATRARRAVRVDVLANDLDADGDRLRVKSIVRAPYYGSAKLEGGEIVY
                     RARRGSGGRADRVTYKVSDGNGGTALATLEVRVKR"
BASE COUNT          575 a         1313 c         1271 g          534 t
ORIGIN      
        1 gaccagttcg acgagccgaa cgacatcaca gtgttcgtca tccaccacca tctcgtcccc
       61 atcccgggga cggggcggga gcgcaacatc atcctggacg ccggcgacgt gctggccgag
      121 ctgacccagc tcgacatcga cgtcgcgctc tcggggcaca agcacgtgcc gtacttctgg
      181 gggctgaacg ggatgctgat ctgcaactcg agcacggcga cgacgaagcg gctgcgcggc
      241 ctcacgccac cgtcgtggaa cgagctgcag atagacgcgt cgacgatcaa gtgctacacg
      301 cactaccggg accgccgcga gctctcgttg atcttcagcc tccggacgcg cacgatgacg
      361 cgcgaggcct tctacctgac ggagaagttc ctggagtcga accagctgct gatggaccag
      421 cgcagcgtgg tgggctaggc ccggcctccg taagcggcgc aagccccttg tcccccggcc
      481 cccggccccc tagattgctt gggttgcggc ccccctagcg ccgaggagaa accgtgcccg
      541 agaatcgccc ccggatccgt ctcgccctgt tcggcgcgct cctcgcgctc ccgctcgtcg
      601 cggtcccgac caacgtcctc agcgctacgg cgttctgcga ccccgcggac gatccccacg
      661 gcgtcacgtc ggcggcgagg ggcagctcga ccgcccgcga gcccgagggg atgaccggcg
      721 aggaggtccc gccgacgcac aagcgcccga agaagttctc ggcgacgatc cccgtctact
      781 tccacgtgat cagcgcgggc acgactcccg aggccgggaa cgtcaccgac gcccagatcg
      841 ccgcacagat gaccgcgctc aacaagtcgt tcggcggctc ctacggcggg ttcaagaccc
      901 cgttcacgtt ccggctcgaa ggggtcaccc ggacggtcaa cgccgagtgg ttcaacatgg
      961 gctacggctc gaaggccgag cgcgacgcga aggcggcgct gcgacgcggt ggcgcgaacg
     1021 ccctcaacgt ctactcgacg gcaggcggcg ggctcctcgg atgggcgacg ttcccctcgt
     1081 cgtatcgcga gcaccccgag cgcgacggga tcgtcgtcca cgcgggttcg ctcccgggcg
     1141 gtcacgtcac gaggttcaac ctcgggttca ccgcgacgca cgaggcgggc cactggctgg
     1201 ggctcttcca caccttccag ggcggctgca acgccaacgg cgactacgtc gccgacacgc
     1261 cggcgcagcg catcgccacc agcggctgcc ccgagggcca ggactcgtgc tcggagccgg
     1321 gcttcgaccc gatccacaac ttcatggact actccgacga cgcctgctac acgcagttca
     1381 cgagcgggca gtcggcccgg atggtcgacc agttcacgta ctaccggagc tgatcgcgat
     1441 gcttgaaccg accgaccccg gggaaagaac ggcgccgtga gaacgagtct gcggatcgcg
     1501 ttgcctctgc tcgtcgtggc atccctcgtc cttccggcgg cccccgcggc cgtcgcgcag
     1561 gacgctcccg gggtgacgct gcacgccggg cgcaagatca tggagttcgg ccagagcgtg
     1621 atcctgcgcg gcgccgtcga cccccccgcc gagggcgaga ccgtgtcgat cgtcgacgac
     1681 accggtcacg aatgggcgac gaccaccacc gacgagaagg gccgcttcca cgtcaaggtc
     1741 gcgcctcgcc gcaccaggca gttccacgcg gtgtgggtcg cagtgcctag cgagcccgtg
     1801 accgtcaagg tccgcccccg cgtcaacgtt tcgcttcgtg cggtgaggct gttcggcaag
     1861 gcgcgcatcg tagggaccgt cgcgccggtg caggagtcgg gccgcgtggc cgtgacgctc
     1921 cgccgcgacg ggaagtccgc gtggacgcgc cgggtcgagc tgcgcaaggg ccgcaagttc
     1981 tcgacgacgt tcgagatcgg caagatcggc aggttcgccg cgatcgcgtc gttcaccgac
     2041 gaagcaggag tgcgcggcgg cgaatggtcc gacgcgaagt cgacgccgct cccctatctc
     2101 tctgtgggcg cccgcggcgt gtacgtgcgt ctgctcgagc agcgcctgtg cgagctgagg
     2161 tacaagctgc tcggttgcaa ccgccgcttc gggatcgaca cggccgacgc catgcgggcg
     2221 ttcaacaaga tcgagggccg ggcccggctc ggcaccgtcg acggcccgac ctgggcggcg
     2281 ctggcccggg cgaacgtggc gaagccgcgg cacgggggca agggcttcca catcgagatc
     2341 gaccagacga agcaggtcgt cctgatggtc gaggacggga aggtgaccca cgtcctccac
     2401 acctcgaccg gggccggcgg cgcgacccgg gacgggacgt tccacgtcta ccgcaagctc
     2461 gagggctaca gcccgaaccg cctctactac ccgagctact tcgacgggct gagggccttc
     2521 cacggatggc ccgaggtgcc gacgtacaac gcgagccacg ggtgcgcgcg ggtcccgatg
     2581 tggtcggcga cgtgggtgta cggcaaggcc gacatcggca cgaccgtgta cgtctaccac
     2641 tgacgctcta gacgccaccc tgctgcggcc cccgctaccg gtggaaccgg ggccacaggt
     2701 tcgccacgct gttcgaagag cgcgtgctca tgtcagaaga tgcgggccat tcgccgctaa
     2761 cgcttgacgc gtacctccag cgtcgccagc gcggtgccgc cgttgccgtc cgagaccttg
     2821 tacgtcacgc ggtcggcgcg tcctccgctg ccgcggcggg cgcggtagac gatctcgccg
     2881 ccctccaact tcgcggagcc gtagtagggc gcacggacga tcgacttcac gcgcaggcgg
     2941 tcgccgtcgg cgtcgaggtc gttcgccagc acgtcgaccc tgaccgctcg ccgcgcccgc
     3001 gtcgccgcgg agtcgtcccc cgcccgaggc gcgtcgttgc gtggttcgac cgtgaccgtc
     3061 acacgtccgg gcgcagaggc cccgaacgcg gagtacgtga acgagtcgga gccgtggaag
     3121 tcggggtccg ggacgtagac gacctcgtcg cccgagccca ggctgaccgt cccgtgggcc
     3181 ggctcgccga cggccaagag gtctccgata ccgctgtcgt tcgagagcgg cgttatgcgg
     3241 gcgctcgtgt cctcggggac ggtcacggag tcgtccgcga cgagcggcgc gcccgggacc
     3301 gacggaggag tcggagtcgg agtcggagtc ggagtcggag tcgggctcgg caggggaggg
     3361 ggcggaaccg gagccggtga cgggacctgc gcgacgtccc cggcctcgac cctcagaccg
     3421 aagatctggg cccagtacgg cgtcccgtcc gcgttcaccg cggcccccac gccgatgacc
     3481 ttgtacgacg ggttctcgat gttgcgccgg tgtccggcgg agtcgagcca cccctccacg
     3541 acggcctgcg gcgtccggta gccgtacgcg atgttctcgc cggccccgct ggagtagccg
     3601 cagtcgcgga tccgctggtc ccaggttcgc cccaccggag gggcggggtc gtcgtgcgtc
     3661 atgtactcga agcccgccat gtgcgcggcc ttc
//
LOCUS       DASQDO010000338         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_954193, whole genome shotgun sequence.
ACCESSION   DASQDO010000338 DASQDO010000000
VERSION     DASQDO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954193"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1683)
                     /locus_tag="VEV43_09525"
     CDS             complement(253..1683)
                     /locus_tag="VEV43_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYP23802.1"
                     /translation="MRLAFWRKEPDPFAPDAMDRTTRGRLRRRRRRRWQIAGITLSLL
                     LLAGTGTLKYLYDRLEDIVQEPVPEVKAEEDELDPFNALLVGSDSREGLTEEEQLAFG
                     AEEVGGQRADTIILAHVDPADNRVTMIQFPRDLYVPIDGAGSNKVNTALMNGPSPLVR
                     TIEDLTEIEINHYAQINIAGFRDIVDAIGGVEICIPDPIPFDPKTGIEVPEEETGMVK
                     FDGERALRFVRSRNYASGDFERIANQQKFMSAALRKVTSTSTFFRPNRIVALYRAVGD
                     NLVLDEGTKLSRLRSIAQRLRSFDPETYEAYIVPNLGTATNEAGSVVLPDDEAMQVMF
                     DAVAANESPADADGVPDVDPSTIRVGIYNGSGIEGAADSGAFALDEATAHGEKRIDIV
                     EIGNAPRTDFKKTVVRYDDSNLGSLEQAGLIAAALPGARVQERKVPDGVDVMVIVGAS
                     FETQRLVRIEPIELPAPADPPPECRR"
     gene            complement(1761..3152)
                     /locus_tag="VEV43_09530"
     CDS             complement(1761..3152)
                     /locus_tag="VEV43_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HYP23803.1"
                     /translation="MSDFRIEHDTMGEVRVPATALYGAQTQRARENFPISGFAPQPDY
                     VWGMVMIKKAAALANMDTGRLDKEIGEAIVAAADEALSGALAEHFVIDPWQAGAGTSH
                     NMNANEVLASRASQILGEDHRVHPNDHVNLAQSSNDTNPTAVRLATLRVLPKLLVALE
                     GLHRSLDTKAREFDGIVKTARTHLQDAVPIRMGQEFSGYAAAIEKAIDRIRDAGKSLE
                     EMNLGGTAAGTGINAEPEYIEGVVQHLRDLTGFDLRQGENFFRLTQSGGDMAHFSSAV
                     RNAAVEISRIASDFRLLASGPRSAIAEIILPPVQPGSSIMPGKVNPSMAEMMNMVCFQ
                     VIGCDEVCSLAAHGGQLDLNVFWPLFGFNLPFSMTILANGVTAFTERLVDGIKVDEDR
                     CRELMEQSLMLCTALTDYIGYEAAAGLAKEAYRDRKTIRQVALEHDVMPAEELDKVLD
                     GMPMTEPGVPGKS"
     gene            complement(3176..>3579)
                     /locus_tag="VEV43_09535"
     CDS             complement(3176..>3579)
                     /locus_tag="VEV43_09535"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HYP23804.1"
                     /translation="GGGGSGWVRLLRAPNDIEAHLLAGRLNEAGVEARIVRETGAPSA
                     WLYGASKPWAPVTVMVRQRQLDDARLVLAEVSLEAPDHVREVAVAPGWRVPVVWWATA
                     LTLGVLVTGAALAQVADATFARCHVPILCND"
BASE COUNT          512 a         1243 c         1222 g          602 t
ORIGIN      
        1 acgaacggct ggacatccac cccgacctgg agccgagccc gatcttcgtc gtgccggtca
       61 cggtcgcgat cctcgcgggc gtgacggtga cggtggtgac ggcatggacg gcccgccgcc
      121 tgcagaagcg catggaccgc acgccggtag gcgagatcat gcgcgtctga cgacggatcg
      181 tctcgacgat ccgccaggaa cgacacgacg gtcgtctcga cgatccgcca agaacgacac
      241 gacggatcgt ggctagcggc ggcactccgg gggtgggtcg gccggggccg gcagctcgat
      301 cggctcgatc cgcacgagcc tctgcgtctc gaacgacgcc cccacgatca ccatcacgtc
      361 gacgccgtcg gggaccttgc gctcctgcac acgggccccc gggagcgccg ccgcgatcag
      421 ccccgcctgt tccagcgagc cgaggttgga atcgtcgtaa cgaacgacgg tcttcttgaa
      481 gtcggtgcgg ggagcgttgc cgatctcgac gatgtcgatc cgcttctcgc cgtgcgcggt
      541 cgcctcgtcc agcgcgaacg cgcccgagtc cgcggccccc tcgatcccgc tgccgttgta
      601 gatgccgacg cggatcgtgg acgggtcgac gtccggcacg ccgtcggcgt ccgccggtga
      661 ctcgttcgcg gcgacggcgt cgaacatcac ctgcatcgcc tcgtcgtccg ggagcacgac
      721 ggaccctgcc tcgttcgtcg ccgtcccgag gttcggcacg atgtaggcct cgtacgtctc
      781 ggggtcgaac gaccgcaggc gctgcgcgat gctccgcagc cgcgacagct tcgtcccctc
      841 gtcgagcacg aggttgtcgc cgacggctct gtagagcgcg acgatccggt tcgggcggaa
      901 gaacgtgctc gtcgacgtca ccttccgcag cgccgcggac atgaacttct gctggttcgc
      961 gatgcgttcg aagtcaccgg acgcgtagtt gcgcgaccgg acgaaccgca gtgcccgctc
     1021 gccgtcgaac ttcaccatcc cggtctcttc ctcggggacc tcgatcccgg tcttcgggtc
     1081 gaacgggatc gggtcgggga tgcagatctc gacgccgccg atagcgtcga cgatgtcgcg
     1141 gaaccccgcg atgttgatct gcgcgtagtg gttgatctcg atctccgtca ggtcctcgat
     1201 cgtcctcacc agcggtgagg gcccgttcat cagcgcggtg ttgaccttgt tagagccggc
     1261 gccgtcgatc ggcacgtaga ggtcgcgcgg gaactggatc atcgtcacgc ggttgtcggc
     1321 cgggtcgacg tgggccagga tgatcgtgtc ggcgcgctgc cccccgacct cctcggcgcc
     1381 gaacgcgagc tgctcctctt ccgtgaggcc ctcgcgcgag tccgatccga cgagcagcgc
     1441 gttgaacggg tccagctcgt cctcttcggc cttcacctcg gggacgggct cctggacgat
     1501 gtcctcgagg cggtcgtaga ggtacttcag cgtccccgtg ccggcgagca acagcagcga
     1561 cagcgttatc cccgcgatct gccagcgccg gcgacggcgt ctcctgaggc gcccgcgcgt
     1621 cgtccggtcc atcgcgtcgg gcgcgaacgg atccggctct ttcctccaga aggcaagtcg
     1681 catgcgatcc cgattatcga gcgggggccg cgtctagaca cggacgccct gcgatgaagt
     1741 tgtgaggatt cggtgagcgc ctacgacttg ccggggaccc ccggctcggt catcggcatc
     1801 ccgtcgagca ccttgtcgag ctcctcggcc ggcattacgt cgtgctcgag cgcgacctga
     1861 cggatcgtct tgcgatcgcg gtacgcctcc ttggcgagac cggccgcggc ctcgtagccg
     1921 atgtagtcgg tcagcgcggt gcacagcatc agcgactgct ccatcagctc gcggcagcgg
     1981 tcctcgtcga ccttgatgcc gtcgaccaga cgctcggtga acgcggtgac gccgttcgcg
     2041 aggatcgtca tcgagaacgg gaggttgaag ccgaacagcg gccagaacac gttgaggtcg
     2101 agctggcccc cgtgcgccgc gagcgagcac acctcgtcgc agccgatgac ctggaagcac
     2161 accatgttca tcatctcggc catcgacggg ttgaccttcc ccggcatgat cgacgacccc
     2221 ggctgcaccg gcgggaggat gatctcggcg atcgcgctgc gggggcctga cgcgagcagc
     2281 cggaagtccg aggcgatgcg cgagatctcg accgcggcgt tgcggaccgc gctcgagaag
     2341 tgcgccatgt ccccgcccga ctgcgtcagc cggaagaagt tctcgccctg gcggaggtcg
     2401 aagcccgtca ggtccctcag gtgctggacg acgccctcga tgtactcggg ctcggcgttg
     2461 atgccggtgc cggcggcggt cccgccgagg ttcatctcct cgaggctctt gccggcgtcg
     2521 cggatgcggt cgatcgcctt ctcgatcgcg gcggcgtagc cggagaactc ctggcccatg
     2581 cggatcggga ccgcgtcctg gaggtgcgtg cgcgccgtct tcacgatccc gtcgaactcg
     2641 cgcgccttgg tgtcgaggct gcggtgcagc ccctcgagcg cgacgaggag cttcggcagc
     2701 acgcgcagcg tggcgagccg gaccgcggtg ggattcgtgt cgttgctcga ctgcgcgagg
     2761 ttgacgtggt cgttcgggtg gactcgatgg tcttcaccca agatctgcga tgcgcggctc
     2821 gcgagcacct cgttcgcgtt catgttgtgc gaggtcccgg cccccgcctg ccacgggtcg
     2881 atcacgaagt gctcggcgag cgccccggag agcgcttcgt ccgcggccgc gacgatcgcc
     2941 tcgccgatct ccttgtcgag gcggccggtg tccatgttcg cgagggccgc ggccttcttg
     3001 atcatgacca tgccccacac gtagtcgggc tgcggcgcga agcccgagat cgggaagttc
     3061 tcgcgcgccc gctgggtctg ggcgccgtag agggcggtgg cggggactcg cacctcgccc
     3121 atcgtgtcgt gctcgatcct gaagtcgctc acttggaaaa gagcctcctt gctctctagt
     3181 cgttacagag gatcggtacg tggcagcgcg cgaacgtcgc gtcggcaacc tgcgcgagcg
     3241 cggcccccgt gacgagcacg ccgagcgtca gcgcggtcgc ccaccacacg acggggacgc
     3301 gccatcccgg cgcgacggcg acctcgcgca cgtggtcggg ggcttccagc gagacctccg
     3361 cgagcacgag ccgcgcgtcg tcgagctggc gctgacggac catgacggtc accggcgccc
     3421 acggcttaga cgcgccgtag agccaggcgc tcggcgcgcc cgtctcgcgg acgatccgcg
     3481 cctcgacgcc ggcttcgttc agccgccccg cgagcaggtg cgcctcgatg tcgttcggcg
     3541 cgcgcaggag ccggacccat ccggagcctc cgccccctc
//
LOCUS       DASQDO010000339         5515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1199084, whole genome shotgun sequence.
ACCESSION   DASQDO010000339 DASQDO010000000
VERSION     DASQDO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199084"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1337)
                     /locus_tag="VEV43_09540"
     CDS             complement(9..1337)
                     /locus_tag="VEV43_09540"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HYP23805.1"
                     /translation="MRRSGTLFVALALVLLTAASATARPAWKRRIDKLVRGHSIGVSV
                     AEDGRSLYRYADKTKRIPASNQKMLLSMAALDRLGPDFEVITIVSAQGIRGRVVNGDL
                     WILGRGDPTLTGGGAHGRALPFRPARIAQLAAQIKGAGIRRVRGSVMGSTGYFARDWY
                     APGWKSSFAAYYVALPTALTFEGNVYQGRHVTNPEWRAARALTRRLQAKGVRVAGRPG
                     TGAPPAKTTTVAQVRSAPLQTLLAYTNRQSSNFFAEVTGKRLGASARKPPGTIAKGAA
                     VIAQWTRRHGVEAIARDSSGLSSENRVSPRGLVHLLEEAETKPWIGAFRRGLPVPGQG
                     TLEDRLQGVKMRAKTGTLDGVSALSGWVWMERTDSWGEFSILSSGMSKSQAVEIEDAI
                     VRTLARSARHGTRTTGTLTTWMGLPSWADDSNTRISASVSSGIWTRTFLL"
     gene            complement(1385..1621)
                     /locus_tag="VEV43_09545"
     CDS             complement(1385..1621)
                     /locus_tag="VEV43_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23806.1"
                     /translation="MSEAALPEWEPMLVDEAIEAAERRLRQEDERAHKRAVQLAREQG
                     YHRLAVEIDRPSRTKISPDQLSFFPKDPTLFDRD"
     gene            1758..2144
                     /locus_tag="VEV43_09550"
     CDS             1758..2144
                     /locus_tag="VEV43_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP23807.1"
                     /translation="MREGGNVSGRWTKRLGVGVAAAVAVVGLAAPSLGDTTRVRAVKK
                     DGKFVWNKANFSVAKGDKVVWKNPTDVNHTVTFYSGATKNTTINPGERTSKKFTTRGA
                     KYYRCTLHSTLNDGECNGMCGHIHVN"
     gene            complement(2152..2538)
                     /locus_tag="VEV43_09555"
     CDS             complement(2152..2538)
                     /locus_tag="VEV43_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23808.1"
                     /translation="MARFPKPKAKTEARNEWAASIRERLPEWLDENAPDNARGMISGN
                     GAVEVLIGASKVASFLPTATGLSAYLVGATDTEVKRIERLCERAGVPEEARPPRTSKY
                     VLVKVRDDATLATVAEALRLHCIGRR"
     gene            2598..4817
                     /locus_tag="VEV43_09560"
     CDS             2598..4817
                     /locus_tag="VEV43_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HYP23809.1"
                     /translation="MHSISRWALPLLLAHALALPLPGVPGAAQEPPERHPVIVASLES
                     LTGLVGACEDADAADGDASNGTDLPFRFCDDGAPGEGGGANGIPVPVAYHPTESGDDY
                     TGLPAPATEEEAAAADERDDLQPDEQGDRVTLDVDISLPPATRRVPRGGRPVMVLAHG
                     FGETDARWEAANVQGSTETWHQSNAWFAARGYVVVNYRARGHRNNQEGGASGTAQLDS
                     RRYELNDFQYLAGLLADYDADRVAAGDEPLFSINPKKIAINGGSYGGWLAWLAVTDHK
                     WRSPANAIPMRVGAIVPRFAPTDILESLVSSGHFYDRVPGTKKPFVAPVDPKQALSRS
                     PLGVMKQSIVSGFVASANNHGGDHVHLPQYVYDAYVRLQQGEPYDGDPVLEPLAERLV
                     TDSSAYYQEPFWKRVAKGLKIPIFSAAATTDPLFPAIETVRFYNKLKKLSPRYPIEMY
                     FGDYQHVYSQNKAKEWADVCGEDRHVCTLDDYRDAKGKLRLTRPPANLVHQGVNSRIN
                     RFLDHYLLGARKRPALNAVMSTTTCPANATESLPADEPGLTYAAPTWRALAPQDEVLG
                     WSGGGTTSTLAPDLHGVESDPVARSRNANVPKCYTTSEIESGPNVVQYESEPLKEPFT
                     LLGIPTLTLDYTTTATDYWIAARVYDRQPDGGDMTLVTRGLCRVNTAVNEERDCKVFD
                     LHGNGWRFEKDHTVVIEVTQADTPFLRRDNVISTITYESANIRLPIADESRVADPRA"
     gene            4892..5074
                     /locus_tag="VEV43_09565"
     CDS             4892..5074
                     /locus_tag="VEV43_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23810.1"
                     /translation="MKRLRLFVVLALAAAPLALGAAPASACQPDGPCPCAEDPTKTIN
                     NTWNKLTGDDFIRCTF"
     gene            5089..5262
                     /locus_tag="VEV43_09570"
     CDS             5089..5262
                     /locus_tag="VEV43_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23811.1"
                     /translation="MRKLKGLLVAGVAVGALVLPAAPASACQPENPCPPCDWTTPADG
                     VWTKVFGHKVFYC"
     gene            5274..5444
                     /locus_tag="VEV43_09575"
     CDS             5274..5444
                     /locus_tag="VEV43_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23812.1"
                     /translation="MTKVRMLLVGSLTAAALVAPAAPAHASCQTELGDMCKLVEVVCN
                     TSVGQKFIPRCR"
BASE COUNT          876 a         1915 c         1889 g          835 t
ORIGIN      
        1 cgggggacct acagcaagaa cgtccgggtc cagatgcccg aggagacaga ggcgctgatc
       61 ctggtgttcg agtcgtcggc ccaggacggc aggcccatcc acgtcgtgag cgtcccggtc
      121 gtcctggtgc cgtgacgcgc ggagcgtgcg agcgtccgga cgatcgcgtc ctcgatctcg
      181 acggcctgtg acttcgacat accgctagag aggatcgaga actcgcccca cgagtccgtc
      241 ctctccatcc acacccatcc ggagagggcg gagacgccgt cgagcgttcc ggtcttggca
      301 cgcatcttca cgccctgcag ccggtcctcg agcgttccct ggccgggcac ggggaggccg
      361 cggcggaagg cgccgatcca gggcttcgtc tccgcctctt ccaagaggtg tacgagtccc
      421 cgtggcgaca ctcggttctc ggaagagagc cccgaggaat cgcgcgcgat cgcctcgaca
      481 ccgtggcgcc tcgtccactg cgcgatgacc gcggccccct tggcgatcgt ccccgggggc
      541 ttgcgggcgg acgcgccgag tcgcttgccg gtcacctcgg cgaagaagtt cgacgactgc
      601 cggttcgtgt aagcgagcag cgtctgcagc ggcgccgacc ggacctgcgc gaccgtggtc
      661 gtcttcgcgg gcggtgcgcc ggtgccgggc cggccggcga cgcgcacgcc cttcgcctgg
      721 aggcgccgcg tcagcgcacg tgccgcgcgc cattcggggt tcgtgacgtg ccggccctgg
      781 tagacgttgc cctcgaacgt tagagccgtc gggagcgcga cgtagtacgc ggcgaacgag
      841 gacttccacc cgggcgcgta ccagtcgcgc gcgaagtagc cggtgctccc catgacgctc
      901 ccgcggacgc ggcggatccc ggcccccttt atctgtgcgg cgagctgcgc gatccgggcc
      961 ggccggaaag gcagcgcgcg gccgtgcgcg cctccgccgg tcagcgtggg gtcacctcgt
     1021 ccgagtatcc acaggtcccc gttgacgacg cggccgcgga ttccctgcgc gctcacgatc
     1081 gtgatgacct cgaagtcggg gccgaggcga tcgagcgccg ccatcgacag cagcatcttc
     1141 tggttcgacg cggggatgcg cttggtcttg tcggcgtacc ggtacaggga gcgtccgtcc
     1201 tcggcgaccg agacgccgat cgaatggccc cgtacgagct tgtcgatcct gcgcttccag
     1261 gcgggccggg cggtcgcgga cgcggccgtc agcaggacga gcgcgagcgc gacgaagagg
     1321 gtcccggagc gacgcatccg gggagtttga ccgcggacgg gctcctagtc cagagggggt
     1381 gccgtcagtc ccggtcgaag agcgtggggt cctttgggaa gaacgagagc tggtcggggc
     1441 tgatcttggt gcgggacggc cggtcgatct cgacggcgag gcggtggtag ccctgctccc
     1501 gggcgagctg gacggctcgc ttgtgagccc gctcgtcctc ctggcgcagc cggcgctcgg
     1561 cggcttcgat cgcctcgtcc accagcatcg gctcccattc aggcaacgct gcctcgctca
     1621 tccacccctc cgtttccgtt ctcacgcccg cttcgatcgt cgaagctggg ggagtggagt
     1681 atgtgccaga ggccttgtaa ttcgccggat ttccggacgc ttagaacccc gcgctgggta
     1741 ccttggaaga agcatccatg agggaaggag gcaacgtgtc gggaaggtgg accaagaggc
     1801 tcggagtcgg cgtggcggcc gctgtcgcgg tggtcgggct ggcggctccg tcgctgggcg
     1861 acacgacgcg cgtgagggcc gtgaagaagg acgggaagtt cgtgtggaac aaggccaact
     1921 tctcggtggc caagggcgac aaggtcgtat ggaagaaccc gacggacgtg aaccacacgg
     1981 tgacgttcta ctcgggggcg acgaagaaca cgacgatcaa cccgggtgaa cgcacgtcga
     2041 agaagttcac gacgaggggg gccaagtact accgctgcac gctgcactcg acgctgaacg
     2101 acggcgaatg caacggcatg tgcggccaca tccacgtgaa ctagcccgcg ctcagcggcg
     2161 gcctatgcag tgaaggcgta gcgcttcggc caccgtggca agcgtcgcgt cgtctctcac
     2221 cttcaccagc acgtacttgc tcgtgcgcgg gggccgggct tcctccggga cgcccgccct
     2281 ctcgcacagc cgctcgatcc tcttcacctc ggtgtcggtc gcgcccacga ggtacgcgct
     2341 cagcccggtc gccgtcggca ggaacgacgc gaccttgctg gcgccgatga gcacctcgac
     2401 cgcgccgttg cccgagatca tcccgcgggc gttgtccgga gcgttctcgt cgagccactc
     2461 cggcaggcgc tcgcggatcg acgcggccca ctcgttgcgc gcctcggtct tcgccttcgg
     2521 cttcgggaat ctcgccatgg gccgaggtta gccaaacagc cgcggtaccc ggccccctgg
     2581 gggagaattg agcgcctatg cactcgatct cccggtgggc cctccccttg ctgctcgcgc
     2641 acgcgctcgc ccttcctctc ccgggcgtgc cgggggccgc gcaggagccg cccgagcgcc
     2701 atcccgtgat cgtggcgagc ctcgagtctc tgacgggtct cgtcggcgca tgcgaggacg
     2761 cggacgcggc cgacggcgac gcgtccaacg gcaccgatct cccgttccgc ttctgcgacg
     2821 acggtgcccc gggcgagggc gggggcgcga acgggatccc ggtcccggtc gcctaccacc
     2881 cgacggagag cggcgacgac tacacggggt tgcccgcccc ggcgacggag gaggaagccg
     2941 cggcggccga cgagcgcgac gacctccagc ccgacgagca aggcgaccgc gtcacgctcg
     3001 acgtcgacat cagcctgccg cccgcgacga ggcgggtccc gcgcgggggc cgtcccgtca
     3061 tggtgctcgc gcacgggttc ggcgagaccg acgcacgctg ggaggccgcg aacgtccagg
     3121 ggtcgaccga gacgtggcac cagagcaacg cgtggttcgc cgcgcgcggc tacgtagtcg
     3181 tgaactaccg agcgcgcggc caccgcaaca accaggaggg cggggcgtcg gggacggcgc
     3241 agctcgactc gcgccgctac gagctcaacg acttccagta cctcgcgggt ctcctcgcgg
     3301 actacgacgc cgaccgggtc gccgcgggtg acgagcccct gttctcgatc aaccccaaga
     3361 agatcgcgat caacggaggg tcgtacgggg gctggctcgc gtggctggcg gtcaccgacc
     3421 acaagtggag gtcgccggcg aacgcgatcc cgatgcgcgt cggcgcgatc gtcccgcgct
     3481 tcgccccgac cgacatcctc gagtcgctcg tgtcgagtgg ccacttctac gaccgcgtgc
     3541 cggggacgaa gaagccgttc gtcgccccgg tcgacccgaa gcaggcgttg tcgcgttcac
     3601 cgctcggcgt gatgaagcag tcgatcgtgt ccggcttcgt cgcctccgcg aacaaccacg
     3661 gaggcgatca cgtccacctg ccgcagtacg tctacgacgc gtacgtgcga ctccagcagg
     3721 gagagccgta cgacggcgac cccgtgctcg agcctctcgc cgagcgcctc gtcaccgaca
     3781 gctccgcgta ctaccaggag ccgttctgga agcgcgtggc gaagggcctg aagatcccga
     3841 tcttctcggc ggccgcgacg accgacccgc tgttccccgc gatcgagaca gtgcgcttct
     3901 acaacaagct caagaagctc tcgccgcgct acccgatcga gatgtacttc ggcgactacc
     3961 agcacgtgta ctcgcagaac aaggccaagg aatgggcgga cgtctgtggt gaggaccgtc
     4021 acgtgtgcac gctcgacgac taccgcgacg cgaagggcaa gctgcgtctg acgcggcccc
     4081 ctgcgaacct cgtgcaccag ggcgtgaaca gccgcatcaa tcgcttcctc gaccactatc
     4141 tgctcggggc gcgcaagcgc ccggcgctca acgcggtgat gtcgacgacg acgtgccccg
     4201 cgaacgcgac cgagtcgctg ccggcagacg agccgggtct cacctacgcg gcaccgacgt
     4261 ggcgcgctct cgcgccgcag gacgaagtcc tcgggtggag cgggggcggg acgacgagca
     4321 cgctcgcccc ggacctgcac ggcgtcgagt ccgaccccgt cgcaaggtcc cgcaacgcga
     4381 acgtcccgaa gtgctacacg acgtccgaga tcgagtcggg gccgaacgtc gtccagtacg
     4441 agagcgagcc gttgaaggag ccgttcactc tgctgggcat cccgacgctg acgctcgact
     4501 acacgacgac ggcgaccgac tactggatcg ccgcccgcgt ctacgaccgg cagcccgacg
     4561 ggggcgacat gacgctcgtg acccgcgggt tgtgccgcgt gaacaccgcg gtgaacgagg
     4621 agcgcgactg caaagtcttc gacctgcacg gcaacggctg gcgcttcgag aaggaccaca
     4681 cggtcgtgat cgaggtgacc caggccgaca cgccgttcct gcgccgcgac aacgtgatct
     4741 ccacgatcac gtacgagtcg gcgaacatcc gcctaccaat agccgacgag agccgggtgg
     4801 ccgaccccag ggcctgacct tttcgggcag gagatcggcc cccttggtat aagtcctcca
     4861 cgtttgtccg aattcaacgt gggaggtcaa gttgaagcgt ctccgcctgt tcgtcgtcct
     4921 cgccctcgct gccgcgcccc tcgccctcgg cgccgcgccc gcatcggcgt gccagcccga
     4981 cggaccgtgt ccgtgcgccg aggatccgac caagaccatc aacaacacgt ggaacaagct
     5041 gaccggcgac gacttcatcc gctgcacctt ctaggaaagg actccgagat gcgcaagctg
     5101 aagggtctgc tcgtagccgg cgtcgccgtc ggtgcgctcg tcctgccggc cgcgccggcg
     5161 tccgcctgtc agcccgagaa cccgtgcccg ccgtgcgact ggacgacgcc ggccgacggt
     5221 gtctggacga aggtcttcgg tcacaaggtc ttctactgct aggaggaagc gtcatgacga
     5281 aggttcggat gctcctggtc ggctccctga ccgcggccgc gctggtggcc ccggccgcgc
     5341 cggcgcacgc gtcgtgccag accgagctgg gcgacatgtg caagctggtc gaggtcgtct
     5401 gcaacacgag cgtgggccag aagttcatcc cccgctgccg ttaggtcgcg aagtccgatc
     5461 cgccgggccc tgccattcgg gcccggcggt ttcgcgtccg ggtcgcgaag gtttc
//
LOCUS       DASQDO010000340         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_954251, whole genome shotgun sequence.
ACCESSION   DASQDO010000340 DASQDO010000000
VERSION     DASQDO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954251"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VEV43_09580"
     CDS             <1..222
                     /locus_tag="VEV43_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23813.1"
                     /translation="SDNACSGTTVYRDGLARLRPPGAHAPPEAPEEPSFPRTPADPDG
                     GEAVSWWIPAVAAAAVAGSALAWRRRATA"
     gene            315..782
                     /locus_tag="VEV43_09585"
     CDS             315..782
                     /locus_tag="VEV43_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23814.1"
                     /translation="MDRLSLGEKVLGVSALALFLLSFVGQWVKVEAEGGGLSQAVKLN
                     AWDGYGFLLKLALFLALVAAGLVIARAANANLSIPWANVYRGLAFATLALVALTLLIG
                     PDESGSVSNEIVSVEISRGIALFVGTLLAAAMAVGAWLHSEAPGTTAVDPATA"
     gene            1009..1878
                     /locus_tag="VEV43_09590"
     CDS             1009..1878
                     /locus_tag="VEV43_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23815.1"
                     /translation="MGGRKLLLVAVVCVLAASCAGGEPAGDPGQAAESAPPADVSDRL
                     EELCTPVAEAHDELYEIEDRRDYVEASRALSEAKRDLAHALRDVDAPGVDEYALALDA
                     YAQTLQEQAEATTGGGAASQAEALDAVVATAKAEVRLEEAAEAADLPDECPPPSSVDP
                     DNGVFVAKANRACFDLYEDAGGEPFDTPKTPEEVALTLDLGRRLSAGIARAVRDATSP
                     GVEGIPVKKIIRMNKERFQSIGDVAETFEGGDYGDFRTAARKLAKVSRKAGREMLSVG
                     LVYCAKASSIVPL"
     gene            complement(1882..>2365)
                     /locus_tag="VEV43_09595"
     CDS             complement(1882..>2365)
                     /locus_tag="VEV43_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYP23816.1"
                     /translation="VTIGASAYWFYRRGYWRLALFMVATPLIGGIISTAVKVLVDRPR
                     PELDDPITHAFGKSFPSGHAMTSTVAYGTLLLAFMPMLPPRWRPRAIAGYVALVLLIS
                     ASRLGLGLHYLSDVLGGIVLGAAWLCVSVATFRVWRREQRKRLPEVLEGVEPEVAAEV
                     "
BASE COUNT          356 a          808 c          865 g          336 t
ORIGIN      
        1 agcgacaacg cctgctcggg aacgaccgtc taccgggacg gcctcgcccg gctgcgccct
       61 ccgggtgcgc atgccccgcc ggaagccccc gaagagccgt ctttccctcg cactcccgcg
      121 gacccggatg gcggcgaggc ggtgtcgtgg tggatcccgg cagtcgccgc cgcagcggtc
      181 gcgggctcgg ccctcgcgtg gcgccggcgg gcgacggcct agctcgcgcc ggaaaaatcg
      241 cccaaaggtc gtaggaagcg gatggtcgct ttcctatagt ggcctctacg gacggtgacg
      301 cgaggaggtc tgcggtggac aggttgtcgc tcggcgagaa ggtgctcggg gtcagtgcgc
      361 tcgctctgtt ccttctctcg ttcgtcggac agtgggtcaa ggtcgaagcc gaaggcggcg
      421 gcctctcgca ggcggtgaag ctgaacgctt gggacggcta cgggttcctt ctcaagctgg
      481 ccctgttcct cgcgctggtg gcggccgggc tcgtgatcgc gcgggccgct aacgcgaacc
      541 tctcgatccc gtgggcgaac gtctaccgcg ggctcgcgtt cgcgacgctc gccctcgtcg
      601 ccctgacgct gctcatcggg ccggacgaga gcggcagcgt ctcgaacgag atcgtctccg
      661 tcgagatcag ccgcgggatc gcgctgttcg tcgggacgct gctcgccgct gcgatggcgg
      721 tgggggcctg gttgcactcc gaggcgccgg gcacgaccgc tgtggacccc gccaccgcgt
      781 agccccggca gcgggcccga gatcgcggtc gtcggcgact tcgacgcgag gaacgcgacg
      841 caccgcgcga cgaccgctgc gtcgaggcac tcgtcggtgc cggctccttc gaatggatcg
      901 acaccgacgc ggtgtcgtcg cgcatgtgcg taacgtcctc gggttccgag acgcggacca
      961 cgcggagacg tgggccgcgg cctaggccgc gggttatcgt ctcggcccat gggcgggcgg
     1021 aagctgctcc tcgtcgcagt ggtgtgcgtc ctcgccgcgt cgtgcgccgg aggcgagccc
     1081 gccggcgatc ccggccaggc tgcggagtcg gcgccccccg ccgacgtctc ggaccggctc
     1141 gaagagctgt gcacgccggt tgcggaggcg cacgacgagc tctacgagat cgaggaccgg
     1201 cgtgattacg tggaggcgtc gcgtgctctc tccgaagcca agcgggacct ggctcacgcg
     1261 ttacgggatg tagacgctcc cggggtggac gagtacgcgc tggctctgga cgcatacgcg
     1321 cagactctcc aggagcaagc agaggcgacg accggcggcg gcgcggcgag ccaggccgag
     1381 gcgctcgacg ccgtcgtcgc gacggcgaag gcggaggtcc ggctcgaaga ggcggccgag
     1441 gcggcggacc ttccggacga gtgcccacct ccttcgtccg tcgaccccga caacggcgtc
     1501 ttcgtcgcca aggcgaaccg cgcgtgcttc gacctctacg aggacgccgg cggcgagccc
     1561 ttcgacacgc cgaagacgcc ggaggaggtc gcgctgacgc tggacctcgg ccgccggctc
     1621 tccgctggga tagcccgagc ggtgagggac gcgacgtccc ccggcgtcga ggggatcccg
     1681 gtgaagaaga tcatccggat gaacaaggag cggttccagt cgatcggtga cgttgccgag
     1741 accttcgagg gtggtgacta cggggacttc cgcacggccg cgcggaagct cgcgaaggtg
     1801 agccggaaag ccggtcgcga gatgctgtcg gtcgggctcg tgtactgcgc caaggcgtcc
     1861 agcatcgtcc cgctgtaacc gtcagacctc ggccgcgacc tcgggctcga cgccctccaa
     1921 gacctccggc aagcgctttc gctgctcgcg gcgccagacc cggaacgtcg cgaccgacac
     1981 gcacagccac gcggccccca gcacgatccc gccgaggacg tcgctcaggt agtggaggcc
     2041 gagcccgagg cgcgacgccg agatcaacag gaccagcgcg acgtagccgg cgatcgcgcg
     2101 cgggcgccag cgcggcggga gcatcggcat gaacgcgagc agcagggtgc cgtacgcgac
     2161 cgtcgacgtc atggcatggc ccgacgggaa gctcttgccg aacgcgtgcg ttatcgggtc
     2221 gtccagctcg ggccgaggcc ggtcgacgag gaccttcacc gccgtgctga tgatgcctcc
     2281 gatcaacggc gtggccacca tgaacagcgc gagacgccag tagccgcgcc ggtagaacca
     2341 gtacgcggac gcgccgatgg tcacg
//
LOCUS       DASQDO010000341         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_771209, whole genome shotgun sequence.
ACCESSION   DASQDO010000341 DASQDO010000000
VERSION     DASQDO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771209"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VEV43_09600"
     CDS             <1..889
                     /locus_tag="VEV43_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYP23817.1"
                     /translation="CQQDPGVIGATDYEELLTDQSLQGVILATPVRTHAPLAAKALAA
                     GVNVLVEKPLATSYADAYSLIETAEQRGLLVMAGHTFLHSPPVQAVERLIAEGEVGKP
                     LYAQSSRVNLGIHQSDVSVIWDLAPHDLSILVYWLKETPVAVSAMGKSVHGFGPADVA
                     FVDLQFPSGCIANVHLSWLAPTKMRRTTLVGDKRMVVYEDTNVEEPVKVYDKGVNVPA
                     PEDFGQYKTSYRSGDVVSPRVGTWEPLRAELGEFLGRVGRGETPNGREETALSVVATI
                     EAAEESLRASGRVVEVRRA"
     gene            complement(973..1245)
                     /locus_tag="VEV43_09605"
     CDS             complement(973..1245)
                     /locus_tag="VEV43_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23818.1"
                     /translation="MIDKELELNEQGHARRRFLKQAATVAWASPFIVTMMSRGAQAQQ
                     TTVCGELDPNESGVCVETAPCGTAQFCVPSPVLGNPTRCACASTPG"
     gene            complement(1317..>1604)
                     /locus_tag="VEV43_09610"
     CDS             complement(1317..>1604)
                     /locus_tag="VEV43_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23819.1"
                     /translation="PDPVVVGLIPATAMPHSEAIQYRPSLLVFPRYTEGAEPSSRRLT
                     AAETVAWCGDNLHSRGPGEPGVLPAVARLGTSTPGYAVRYGSTADALELLE"
BASE COUNT          258 a          546 c          567 g          233 t
ORIGIN      
        1 ctgccagcag gacccaggcg tgatcggcgc gacggactac gaggagctcc tcaccgacca
       61 gtcgctccag ggcgtgatcc tcgcgacgcc ggtgcggacg cacgccccgc tggcggccaa
      121 ggcgctcgcc gctggcgtca acgtcctcgt cgagaagccg ctcgccacgt cgtacgccga
      181 cgcgtactcg ctgatcgaga ccgccgagca gcggggtctc ctcgtcatgg ccgggcacac
      241 gttcctccac agcccgccgg tccaggcggt tgaacggctc atcgccgagg gcgaggtggg
      301 caagccgctc tacgcgcaga gctcccgcgt caacctcggg atccaccagt ccgacgtcag
      361 cgtgatctgg gacctcgccc cgcacgacct gtcgatcctc gtctactggc tgaaggagac
      421 gccggtcgcg gtatcggcga tgggcaagtc ggtccacggg ttcggcccgg ccgacgtggc
      481 gttcgtcgac ctccagttcc cgtccggctg catcgcgaac gtccacctct cgtggctcgc
      541 gccgacgaag atgcgccgca cgacgctcgt cggcgacaag cgcatggtcg tctacgagga
      601 cacgaacgtc gaagagcccg tcaaggtcta cgacaagggc gtgaacgtgc cggcccccga
      661 ggacttcggc cagtacaaga cctcctaccg ctcgggcgac gtggtgtcgc cgcgggtggg
      721 gacgtgggag ccgctgaggg ccgagctcgg cgagttcctg ggccgcgtcg gccggggcga
      781 gaccccgaac ggacgcgagg agaccgcgct gtcggtcgtc gcgacgatcg aggccgccga
      841 ggagtcgctc cgggcgagcg gccgggtcgt ggaggtccgg cgggcctaga ccgagcgagg
      901 actacacaac ggcggtatac gccggtaatg aaaagagccc cctcgcgggg gctctttttc
      961 gtctacgaag gtctagccgg gggtcgacgc acaggcgcaa cgcgtcgggt tcccgagcac
     1021 cgggctcggg acgcagaact gagccgtccc gcaaggcgcg gtctcgacgc acacgccgga
     1081 ctcgttgggg tccagctcgc cgcacaccgt ggtctgctgc gcttgcgcgc cgcgactcat
     1141 catcgtcacg atgaaggggc tggcccatgc aaccgtcgca gcctgcttga ggaagcgccg
     1201 ccgcgcgtgg ccttgttcgt tgagctccag ttccttgtcg atcacaaacc gcccctctcg
     1261 gtgcccatgc tgaatagtga gggagattat tccatcgaac gggcgtccac aagacactac
     1321 tccaaaagtt ccagtgcatc tgcggtcgag ccgtagcgga cggcgtagcc gggggtcgac
     1381 gtgccgagcc gggcgacggc ggggagcacg cccggttccc ccggcccccg ggagtggagg
     1441 ttgtccccgc accaggcgac ggtctccgcc gccgtcaggc ggcgggaaga aggctcggcc
     1501 ccctcggtgt agcgggggaa cacgagcagc gagggccggt actggatcgc ctcggagtgc
     1561 ggcatggcgg tcgcagggat taggccgacc accacggggt cggg
//
LOCUS       DASQDO010000342         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_159499, whole genome shotgun sequence.
ACCESSION   DASQDO010000342 DASQDO010000000
VERSION     DASQDO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159499"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VEV43_09615"
     CDS             <1..1416
                     /locus_tag="VEV43_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HYP23820.1"
                     /translation="EADVSVKLVSSQGVGSVAAGVVMGLADVVHVSGGDGGTGASPLS
                     SIKTAGMPWELGLAEAHAALAANGLRGRARLRVDGGLKTGRDVVVAALLGADEFSFGT
                     ALLVAEGCILVRTCHRDTCPVGIATQNPALRAKFAGTPEMVARYLLLVAEEARTLLAG
                     LGLRSIAEAIGRTDLLEQKVTGDARADALDLSPLLERAPAAVSTGRHHAVRSELGDRL
                     HDDVVDDVLAGRVTQVSYPITTAERAGGARLSAALEKRPRHGRGLAVARFEGHAGQSF
                     GAFLAEGVELVLEGDANDYVCKGMAGGRVVVRPPDDDTGDPVLVGNTVLYGATGGTLF
                     VAGRAGERFAVRNSGAVAVVEGTGDHACEYMTAGAVVILGPTGRNVGAGMSGGEAYVF
                     DPDDLLAANLNTQLVAAYEPTLSQLDSLRRVIERHARATGSGRAAALLRHWEQAAPRF
                     RRVAPMAEVARLEALFEGSVA"
     gene            complement(1384..2100)
                     /locus_tag="VEV43_09620"
     CDS             complement(1384..2100)
                     /locus_tag="VEV43_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYP23821.1"
                     /translation="MSTKAALRLLSDEVAACRACPRLVAWREKTAREKRASFADEEYW
                     GRPIPGYGDPDARILVVGLAPAAHGGNRTGRIFTGDRSGDWLFASMYRQGLTNQPHSV
                     HRDDGLELVDAYVAAAVRCAPPDNKPAIDERDRCLPYLVREVQLLPALRVVVALGGFA
                     WDAWLRTARALGHTPPRPKPRFGHLAEAEVGPHTLLGSYHPSQQNTFTGRLTEEMLDA
                     VFARARALAGLGDAPFEQRL"
BASE COUNT          286 a          757 c          814 g          300 t
ORIGIN      
        1 gaagcggacg tctcggtgaa gctcgtctca tcccaaggcg tcgggtccgt cgccgcgggc
       61 gtcgtcatgg gtctcgccga cgtcgtgcac gtctccggcg gcgacggcgg caccggcgcg
      121 agccctcttt cgtcgatcaa gaccgcgggg atgccgtggg agctcgggct cgccgaggca
      181 cacgccgcgc tcgcggcgaa cggtctgcgg ggccgcgccc gcctgcgcgt ggacggaggg
      241 ctgaagaccg gacgcgacgt cgtcgtcgcc gctctcctcg gagcggacga gttctcgttc
      301 ggcacggcgc tgctcgtcgc ggagggatgc atcctcgtgc gcacctgtca ccgcgacacg
      361 tgcccggtcg gtatcgcgac gcagaacccc gcgctgcggg cgaagttcgc cggcacgccg
      421 gagatggtcg cccgctacct gctgctcgtg gcggaggagg cgaggacgct gctcgcgggc
      481 ctcggcctgc ggagcatcgc cgaggccatc gggcgcacgg acctgctgga gcagaaggtg
      541 accggcgacg cccgcgccga cgcgctggac ctgtcgccgc tgctcgagcg ggcccccgcc
      601 gccgtctcga cgggaaggca tcacgcggtc aggtccgagc tcggtgaccg gctccacgac
      661 gacgtcgtcg acgacgtgct cgcgggacgc gtgacgcagg tctcgtaccc gatcacgacc
      721 gccgagcggg cgggcggagc tcggctctcc gcggccctcg agaagcgccc ccgtcacggc
      781 cgcggcctcg ccgtcgctcg cttcgagggc cacgccggcc agtccttcgg cgcgttcctc
      841 gccgagggcg tcgagctcgt gctcgagggc gacgccaacg actacgtctg caaggggatg
      901 gccgggggcc gcgtggtcgt gcgtcccccg gacgacgaca ccggcgatcc ggtgctcgtc
      961 ggcaacacgg tcctgtacgg cgccaccgga gggacgctgt tcgtcgcggg gcgcgcgggc
     1021 gagcgcttcg ccgtgcggaa ctccggcgcc gtcgccgtcg tcgaggggac gggtgaccac
     1081 gcgtgcgagt acatgaccgc cggcgccgtc gtgatcctcg ggccgacggg ccgcaacgtc
     1141 ggggcgggga tgagcggcgg cgaggcctac gtcttcgatc ccgacgacct cctcgcggcg
     1201 aacctcaaca cacagctggt agcggcctac gaaccgacgc tctctcagct ggactcgctg
     1261 cggcgcgtga tcgagcggca tgcacgggcg acgggatcgg ggcgtgccgc tgcgctgctg
     1321 cggcactggg agcaggccgc gccgcgcttc cgcagggtcg cgccgatggc cgaggtcgcg
     1381 cggctagagg cgctgttcga agggagcgtc gcctagcccg gccagagccc gggcgcgcgc
     1441 gaagaccgcg tcgagcatct cctcggtcag ccggccggtg aaagtgttct gctggctcgg
     1501 gtggtacgag ccgaggagcg tgtgcggccc cacctcggcc tcggcaaggt ggccgaagcg
     1561 cggtttgggc cgcgggggcg tgtgcccgag ggcgcgcgcg gttcgcagcc acgcgtccca
     1621 ggcaaagcct ccgagcgcga ccacgactcg cagggcaggc agcagctgca cctctctcac
     1681 gaggtacgga aggcagcggt ccctctcgtc gatcgcgggc ttgttgtccg ggggcgcgca
     1741 gcggacggcc gcggcgacgt acgcgtcgac gagctcgagc ccgtcgtctc tgtggacgga
     1801 gtgcggctgg ttcgtgaggc cctgccggta catcgaggcg aacagccagt cgcccgaccg
     1861 gtcgccggtg aagatgcggc ccgttcggtt cccgccgtgg gccgccggcg cgagcccgac
     1921 gacgaggatc cgggcatcgg gatcgccgta gccggggatc gggcggcccc agtactcctc
     1981 gtccgcgaac gacgcccgct tctcccgggc ggtcttctcg cgccacgcga cgagcctcgg
     2041 acaggcccgg cacgccgcga cctcgtccga cagcaaccga agcgcggcct ttgtggacat
     2101 tccaaaagta tttcggttca atggcaggtg cggagggtag ttggtcgaac acacggt
//
LOCUS       DASQDO010000343         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_832092, whole genome shotgun sequence.
ACCESSION   DASQDO010000343 DASQDO010000000
VERSION     DASQDO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832092"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1352)
                     /locus_tag="VEV43_09625"
     CDS             complement(138..1352)
                     /locus_tag="VEV43_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HYP23822.1"
                     /translation="MAISPKTKVKVNVVAFVVLGVGLSYAMATQVLSVLQDRYSVVAT
                     FPDAGGVFTNQEVTYRGITVGQVGKMSVVEDGVDIELLIDEGTKIPEDDVEARVMFKS
                     AVGEQFVDLLPGSDDGPYLADGSRIPLEQTSIPVSTQDLLATLQSVLEGVPPEALAGA
                     IDSAGEGLAGRGDDIATILDSMARLAGLFGDRAPEVQGILRNGTKVGEAFVASREDFA
                     AAVRDLVIVSETLQGSTDELRRLLVGANVTSEELVGLIRESRPDLHQTIADLAEINDI
                     QVEKSNALKALFEYLPRGLGNVVKTFEPATGMIRFGLVTDTANHACSYGTERRQPEDR
                     GPHLPPRNARCSNPPDGEEQVPGTAAVPPVEPAAPDTTGILGLDEAAAGDSRLPNRMS
                     DWSWTLVYLNSL"
     gene            complement(1352..2428)
                     /locus_tag="VEV43_09630"
     CDS             complement(1352..2428)
                     /locus_tag="VEV43_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HYP23823.1"
                     /translation="MTARRMVLLLVACVAGTSCAALGYAGKCEGTSVIANFEQVGDLV
                     EAANVQSSDVVVGTVQKIELKGWEAAVSMCLDADEQIPADVEARIRTTSLLGEKFVDL
                     RPRTEGPPYLEDGAVLGIEDTGKASELEEVFAELAGILGSGNLEDLNRFTASQAEILR
                     DNAGELRTVLAELNEFTGTLATRKDDIAGAIDSLDRVARAVVDEQGVLKRFLDSFADS
                     SGVLADQKGSLNDLLFSLDRFTRISVQLLEATESGLDEQFRELRPVLRTVVRNSERVR
                     ETVQTLATFSEYFPESMPGDYLQLDVCQALPEDYEQGTTCPQSDNGDPGDGLSRGRDG
                     RNRADEMSDLERLLRKPLERRGRS"
BASE COUNT          353 a          874 c          866 g          388 t
ORIGIN      
        1 cgaaggctcc cgcggccgcg agcgcgaaca cgagggccgc gacgaggatc gggaccttgc
       61 gcgacggtcg ttcggacgcg gccggctgca cgccttccac gggggtgtcc atcgaacgat
      121 cctacgttcg cgggcggcta cagcgagttg aggtagacga gcgtccacga ccagtcgctc
      181 atccggttcg gcagacggga gtcgcctgcc gcggcctcgt cgaggcccag gatcccggtc
      241 gtgtcgggag ctgcgggctc caccgggggc accgcggcgg tccccggcac ctgctcctcg
      301 ccgtcgggcg ggttcgagca acgcgcgttg cgcggcggga ggtgggggcc gcggtcctcc
      361 ggctgacgcc gctcggtccc gtagctgcag gcgtggttcg cggtgtccgt gacgaggccg
      421 aagcggatca tccccgtggc cggctcgaac gtcttcacca cgttgccgag cccccgcggc
      481 aggtactcga acagcgcctt cagagcgttc gacttctcga cctggatgtc gttgatctcg
      541 gcgaggtccg cgatcgtctg gtggaggtcc ggccggctct cgcggatgag gcccacgagc
      601 tcctcggagg tgacgttcgc cccgacgagg aggcgacgca gctcgtccgt gctcccctgg
      661 agcgtctcgc tcacgatcac gaggtcgcgc accgccgcgg cgaagtcctc gcgcgacgcg
      721 acgaacgcct ctccgacctt cgtgccgttg cggaggatgc cctgcacctc gggggcgcgg
      781 tcgccgaaga gcccggcgag ccgggccatc gagtcgagga tcgtcgcgat gtcgtcgccc
      841 cgcccggcca ggccctcgcc cgccgagtcg atcgcgccgg cgagagcttc gggcggcacg
      901 ccttcgagca ccgactggag ggtcgcgagc aggtcctgcg tgctgacggg gatcgacgtc
      961 tgctcgagcg ggatgcgcga cccgtccgcg aggtacggcc cgtcgtcaga gccgggcagc
     1021 aggtcgacga actgctcgcc gaccgcgctc ttgaacatca cgcgtgcctc gacgtcgtcc
     1081 tcggggatct tcgtcccctc gtctatgagc agctcgatgt cgacgccgtc ctcgacgacg
     1141 gacatcttgc cgacctgccc gacggtgatg ccgcggtagg tcacctcctg gttcgtgaac
     1201 accccgccgg cgtcggggaa cgtcgcgacg acgctgtagc ggtcctggag caccgacagc
     1261 acctgcgtcg ccatcgcgta ggagaggccc acgccgagga cgacgaacgc gacgacgttg
     1321 accttgacct tggtcttcgg cgagatcgcc atcaggatcg ccctcgcctt tcgagcggct
     1381 tgcgcaggag gcgctcgagg tcgctcatct cgtcggcccg attgcggccg tcacgcccgc
     1441 gggagaggcc gtcgccgggg tcaccgttgt ccgactgcgg gcacgtcgtg ccctgctcgt
     1501 agtcctcggg cagcgcctgg cacacgtcga gctgcaggta gtcgcccggc atgctctccg
     1561 ggaagtactc cgagaacgtc gcgagcgtct ggacggtctc gcgcactcgc tcgctgttgc
     1621 gcacgaccgt ccgcagcacg ggccggagct cacggaactg ctcgtcgagg ccggactcgg
     1681 tcgcctcgag cagctgcacg ctgatgcgcg tgaagcggtc gagggagaac aggagatcgt
     1741 tcagcgagcc cttctggtcc gccagcacgc ccgacgagtc ggcgaacgag tcgaggaagc
     1801 gcttgagcac accctgctcg tcgacgaccg cgcgcgcgac gcggtccagc gagtcgatgg
     1861 caccggcgat gtcgtccttg cgtgtagcca gcgtgccggt gaactcgttc agctccgcga
     1921 ggaccgtgcg gagctcaccc gcgttgtcgc gcaggatctc ggcctgggac gcggtgaacc
     1981 ggttgaggtc ttcgaggttg cccgagccga ggatgcctgc gagctcggcg aagacctcct
     2041 cgagctccga cgccttgccc gtgtcctcta tgccgaggac ggccccgtcc tcgaggtagg
     2101 gaggcccttc cgtgcgcggg cggaggtcga cgaacttctc gccgaggagc gaggtcgtgc
     2161 ggatgcgtgc ctcgacgtcg gcggggatct gctcgtccgc gtcgaggcac atcgacaccg
     2221 ccgcctccca gcccttgagc tcgatcttct ggaccgtccc gacgacgacg tccgaggact
     2281 ggacgttcgc cgcctcgacg aggtcgccga cctgctcgaa gttcgcgatg acgctcgtgc
     2341 cctcgcactt gcccgcgtag ccgagggccg cgcaggacgt gcccgcgacg caggccacga
     2401 gcagcaacac catgcggcgc gcggtcacga cagccccacg aagtcgaaca gcccgtcgat
     2461 ccaaccggcc gcttcctcgc c
//
LOCUS       DASQDO010000344         5468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_587797, whole genome shotgun sequence.
ACCESSION   DASQDO010000344 DASQDO010000000
VERSION     DASQDO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587797"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VEV43_09635"
     CDS             complement(<1..522)
                     /locus_tag="VEV43_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HYP23824.1"
                     /translation="MPPELISSPSNPLLKRIRKLRQRKHRTAEGALFAEGIAPVWQAL
                     ESGAGIEVVVTAPELLTSDKARALVESAAAPVVEVTRGAFESIADRDNPSGLAAIVSI
                     PQVSLADVELGPAPLVVGLDEVGNPGNLGTVVRTADAAGAAAVVVAGESADPWHPGAV
                     KASMGTVFSTPVVT"
     gene            complement(527..928)
                     /locus_tag="VEV43_09640"
     CDS             complement(527..928)
                     /locus_tag="VEV43_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HYP23825.1"
                     /translation="MAIPISEPSKILLEEPAGSLEELGDAQSAERAAEVAAKHPACLT
                     AWAILGEEALAQQRFVDAYAYFRVGYHRGLDGVRKAGWKGEGVVPWSHVPNRGFLRSL
                     AGLGKAAEAVGEPDEATRCEEFLRQLSPDAP"
     gene            complement(946..2151)
                     /locus_tag="VEV43_09645"
     CDS             complement(946..2151)
                     /locus_tag="VEV43_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23826.1"
                     /translation="MLVRNTVIAMITVLACAWFPAGAAHAQNTTCYGRAATITGTKRA
                     DRLRGTPGPDVIVAAGGDDRITGLGGNDRICAGTGEDVVDAGSGRDRVLGGLGDDEIR
                     GGAGRDRLDGDDQDDELHGNGGNDRLILGRIGREVEYAYGGLGRDVIVSESDAVTRAY
                     GNKGSDRLVGHARGDFLDGGPGDDRIESGTATFETSGAGVTVDVEAGTATGAGTDVLV
                     DVLGVVGSPHDDTITGGRRAEWIQGMDGDDTLYGALGDDNVAGGKGSDVVWGTRGADV
                     LWDAESECHFGCDADESADEFDGGPGEDIVTYEGHRSGVTVDLQSPTGEDGDTITEVE
                     NLAGSWFDDHLLGTGGSNELYGLDGDDHVEARESDDLLDGGDGTDTLDGGLHTDACHN
                     GEVLIACEE"
     gene            complement(2214..2405)
                     /locus_tag="VEV43_09650"
     CDS             complement(2214..2405)
                     /locus_tag="VEV43_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23827.1"
                     /translation="MNGQTAPALPATPETNLMLVGPLGEALVALACKRFEEAVQSVLD
                     APLDVARVAEWTRLRALNV"
     gene            complement(2505..2587)
                     /locus_tag="VEV43_09655"
     tRNA            complement(2505..2587)
                     /locus_tag="VEV43_09655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2552..2554),aa:Leu,seq:gag)
     gene            complement(2646..4322)
                     /locus_tag="VEV43_09660"
     CDS             complement(2646..4322)
                     /locus_tag="VEV43_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HYP23828.1"
                     /translation="MKLRRATAALLLLVAPALLGACDDDDPAAANGGDATESGAPAPG
                     TEGTAASPSVLQPGGFTEEDDVLNVATRQPSTLDPMRIQDPGSVLVARQLYEGLTRWD
                     PVQQRVVPAAAQSWDVGGGGRVYTFHLRQGMTFHDGSPVRAADFRFAFDRIAKKSNAS
                     DLAYTLERVQGFPEVNSAGTSDHLTGIRASDDLTLRITLSEPFRGLPAVLTHPGLVPL
                     RAQAVASYDEFLAKPVGNGPFQMAEKWVPGEPVILQDFPGFVETPSLDGIRFVPAPDA
                     AASWPWFVDERIDVAEVPSGQFDAARETYGEDGYKPFLAGYYFGLNVKSGELDRIALR
                     KAISRAIDRREIAESVYKGNMQLPRGIVPAGMPGFQENVCITLCRYSPEIAEKMVADI
                     PPKERRISVQFNREDPHGRVARLVKRDLEAAGFTVRIVGYGFPRYLRLLRDGKQSAYR
                     LGWIAEYPEADVFLSGLFESGSPDNHSGFASPRVDALLAKARRTESEAVRAQTYVQAE
                     KLILAQMPVVPIGSFVTHWAAQPWVDEIEFDVMGGFDAVEVSLEGKPAES"
     gene            complement(4339..4569)
                     /gene="secG"
                     /locus_tag="VEV43_09665"
     CDS             complement(4339..4569)
                     /gene="secG"
                     /locus_tag="VEV43_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HYP23829.1"
                     /translation="MTPLLVIIHSVAAVTLIVFVLLHAGRGGGVSEMFGGGMQTQAMG
                     STVMEKNLDRITIITAIVFGGTTILLAMRLSD"
     gene            complement(4658..>5468)
                     /locus_tag="VEV43_09670"
     CDS             complement(4658..>5468)
                     /locus_tag="VEV43_09670"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYP23830.1"
                     /translation="ISEAVIFDLDREELEGVLAHEVAHLETHDVRVVFVAGLLRDLVA
                     WNPLAHVAFRRLASDREREADRRAATLTARPLAVASGLLKMYDLMKTQLRSVDQGAMA
                     FLRPGRRISRRVSDLIALADGRTLSPPAGRMPYLMAGLLVAGLGLQVGARVATQDMNG
                     FAIVLGDTTVPKAEYWSPAEMRHRIPQHEVAARRSGKAEAARTPRYPELTREFLVKEK
                     NLPEWFEWMAKRAERISTASVAWEGRREWQATPVFRQSSGGVTIYRLSPSS"
BASE COUNT          774 a         1946 c         1919 g          829 t
ORIGIN      
        1 cgtcaccacc ggggtcgaga agaccgtccc catcgacgcc ttgaccgccc ccggatgcca
       61 cgggtcggcc gattccccgg ccacgacgac cgccgcggcc ccggccgcgt ccgcggtccg
      121 gacgaccgtg ccgaggttcc cggggttccc gacctcgtcc aggccgacga ccagcggggc
      181 cgggccgagc tcgacgtcgg cgaggctcac ctgtgggatc gagacgatcg cggcgagccc
      241 cgacgggttg tcgcggtcgg cgatgctctc gaacgcgccc cgggtgacct ctacgacggg
      301 ggccgctgcc gactcgacca gagcgcgcgc cttgtcgctc gtcagcagct cgggtgccgt
      361 cacgacgacc tcgatgccgg cgccggactc caacgcctgc cacaccggtg cgatcccctc
      421 ggcgaacagc gcgccttcgg ccgtgcggtg cttgcgctga cggagcttgc ggattcgttt
      481 gaggagcggg ttcgacgggc tggagatcag ctccgggggc atgccgtcag ggcgcgtccg
      541 gcgagagctg tctaaggaac tcttcgcagc gcgtcgcctc gtccggctca ccgacggcct
      601 ccgcggcctt gccgagcccc gcgagcgaac gcaggaaccc acggttcggc acgtgtgacc
      661 acgggacgac gccctcgccc ttccagccgg ccttgcggac tccgtcgagg ccgcggtggt
      721 agccgacgcg gaagtacgcg tacgcgtcga cgaagcgctg ctgcgcgagg gcctcctcgc
      781 cgaggatcgc ccatgcggtg agacacgccg ggtgcttggc cgcgacctcg gccgcgcgct
      841 cggcactctg tgcgtcgccg agctcttcga gcgagcccgc cggctcctcg agaaggatct
      901 tcgacggttc gcttatcggt atggccatgg gggcatcgta gaacgtcact cctcgcaggc
      961 gatcaagacc tcgccgttgt ggcacgcgtc ggtgtgcaga ccgccgtcga gcgtgtcggt
     1021 cccgtcgccg ccgtcgagga ggtcgtcgct ttcgcgagcc tcgacgtggt cgtcgccgtc
     1081 gagcccgtac agctcgttgc tcccaccggt gccgagcaga tggtcgtcga accacgagcc
     1141 ggccaggttc tcgacttcgg tgatcgtgtc cccgtcctcg ccggtaggcg actggaggtc
     1201 gactgtcaca ccgctccggt ggccctcgta ggtgacgatg tcttccccgg gacccccgtc
     1261 gaactcgtcg gccgactcgt ctgcgtcgca cccgaagtgg cactcggact cggcgtccca
     1321 cagcacgtcg gcgccgcggg tgccccagac gacgtcggaa cccttgccgc cggccacgtt
     1381 gtcgtcgccc agcgcgccgt agagggtgtc gtcgccgtcc atgccctgga tccactcggc
     1441 ccggcggccg cccgtgatcg tgtcgtcgtg cggcgacccg acgaccccga gcacgtccac
     1501 gaggacgtcg gtgcccgcgc ccgtggcggt cccggcctcg acgtcgacgg tcactcccgc
     1561 gcccgacgtc tcgaacgtgg cagtcccact ttcgatccgg tcgtcgcccg gccccccgtc
     1621 gaggaagtcc ccgcgggcgt gcccgacgag acggtccgag cccttgttgc cataggcgcg
     1681 ggtgactgcg tcgctctccg agacgatcac gtcccggccc aggccgccgt aggcgtactc
     1741 gacctcacgg cctatccggc cgaggatcag ccggtcgttg ccgccgttgc cgtgcagctc
     1801 gtcgtcctga tcgtcgccgt cgaggcggtc gcgtccggcg ccgccgcgga tctcgtcgtc
     1861 gccgaggccc ccgagcacgc ggtccctgcc cgaccccgcg tcgacgacgt cctcgccggt
     1921 gccggcgcag atgcggtcgt tcccgccgag tcccgttatc cggtcgtctc ccccggcggc
     1981 gacgatcacg tcggggcccg gcgtgccgcg caggcggtcg gcccgcttcg tccccgtgat
     2041 cgtggcggcg cggccgtagc aggtggtgtt ctgagcgtgc gcggcgccgg ccgggaacca
     2101 ggcgcaggcg agcacggtga tcatcgctat aaccgtgttg cggacgagca tgtgaccccc
     2161 tcgatgagcc ggccgttacg acccactctg agggctacct caccggacgt ctgtcaaacg
     2221 ttgagcgctc ggagcctggt ccactcggct actcgcgcga cgtcgagggg tgcgtcgagc
     2281 acgctctgga ccgcttcctc gaaccgcttg cacgccagcg ccacgagcgc ctccccgagg
     2341 ggtccgacca gcatcaggtt cgtctcggga gttgccggga gagcaggggc cgtctgtccg
     2401 ttcatcgccg ttccccttcc agtcggagcg cgaaaagcgt cctcccgata ctggacggtc
     2461 cgggcgaaac cggacaatac gtaaaacagg acctaggcga agagtgcggt gggggggatt
     2521 tgaaccccca cacccttgcg ggcaccagcc cctcaagctg gcgtgtctac cgttccacca
     2581 ccaccgctcg aaacgaaggc cccgagatcg ctcagggccg tcgccgatca tacaagccgc
     2641 ggccctcagg actccgcggg cttgccctcc agcgacacct ctaccgcgtc gaagccgccc
     2701 atgacgtcga actcgatctc gtcgacccac ggctgagcgg cccagtgcgt cacgaaagac
     2761 ccgatgggca cgacgggcat ctgcgccagg atgagcttct cggcctggac gtaggtctgg
     2821 gcccggacgg cctcggactc ggtccggcgt gccttcgcca gcaacgcgtc gacgcgcggc
     2881 gacgcgaacc ccgagtggtt gtccggcgag cccgactcga acagccccga caggaacacg
     2941 tccgcctccg ggtactccgc gatccagccg agccggtagg cgctctgctt gccgtcgcgc
     3001 agcaggcgca ggtagcgcgg gaagccgtag cccacgatcc tgaccgtgaa gcccgcggcc
     3061 tcgaggtccc tcttcacgag cctcgccacc cggccgtgcg ggtcctcgcg gttgaactgg
     3121 acggagatgc gccgttcctt cggcgggatg tccgccacca tcttctcggc tatctccggc
     3181 gagtagcggc acagcgtgat gcagacgttc tcctggaacc ccggcatccc cgcggggacg
     3241 atcccgcgcg ggagctgcat gttgcccttg tagacggact cggcgatctc gcggcggtcg
     3301 atcgcccggc tgatcgcctt ccgcagggcg atgcggtcga gttcgcccga cttcacgttc
     3361 agcccgaagt agtagccggc gaggaacggc ttgtacccgt cctcgccgta ggtctcgcgg
     3421 gccgcgtcga actgccccga cggcacctcc gcgacgtcga tgcgctcgtc gacgaaccag
     3481 ggccacgagg ccgcggcgtc gggggccggg acgaagcgga tgccgtcgag gctcggcgtc
     3541 tcgacgaagc cggggaagtc ctggaggatc accggctcgc ccggcaccca cttctccgcc
     3601 atctggaacg ggccgttgcc gacgggcttc gcgaggaact cgtcgtaact ggcgaccgcc
     3661 tgggcccgca gcggcaccag gccgggatgc gtcaggacgg cggggaggcc gcggaagggc
     3721 tccgacagcg tgatccgcag cgtgaggtcg tccgaggccc ggatgccggt caggtggtcg
     3781 gaagtcccgg ccgagttcac ctcggggaag ccctggacgc gttcgagcgt gtacgcgagg
     3841 tcggacgcgt tcgacttctt ggcgatgcgg tcgaacgcga agcggaagtc ggcggccctg
     3901 acgggcgaac cgtcgtggaa cgtcatcccc tggcggagat ggaacgtgta gacgcggccc
     3961 ccaccgccga cgtcccagga ctgggccgcc gccgggacga cccgctgctg gacggggtcc
     4021 cagcgcgtca gcccctcgta gagctgccgg gcgacgagga cggagccggg gtcctggatc
     4081 cgcatcgggt cgagcgtgga cggctggcgc gtcgcgacgt tgaggacgtc gtcctcttcg
     4141 gtgaacccgc cgggctggag aaccgacggg ctcgcggcgg tgccctccgt tccgggagcg
     4201 ggtgcgccgg actcggtcgc gtcgccgccg ttcgcggccg ccgggtcgtc gtcgtcgcac
     4261 gcacccagga gcgcaggcgc gacgagcagc aggagggccg cggtggcccg gcggagcttc
     4321 atgtgaggag ggacgcggct agtcggacag ccgcatcgca agaaggatcg tcgtgccccc
     4381 gaagacgatc gcagtgatga tcgtgatccg gtcgaggttc ttctccatga ccgtcgaccc
     4441 catggcctgc gtctgcatgc cgccgccgaa catctcggag acaccgcccc cgcgccctgc
     4501 gtgcagcagc acgaagacga tcagggtcac cgcggcgacc gagtggatga tgacgagcag
     4561 cggggtcatg tgggcacctc ttggggttgg tcgtgatctc ggagacgacc aagggtagcc
     4621 gaatacgccg gtttccggcc gaggccggcg tccggggtca ggaagacggg ctgaggcggt
     4681 agatcgtgac gccgccggag gactggcgga agaccggcgt cgcctgccac tcgcggcggc
     4741 cctcccaggc gaccgaggcc gtcgagatcc gctcggcccg cttcgccatc cattcgaacc
     4801 actcgggcag gttcttctcc ttgacgagga actccctcgt cagctcggga tagcgggggg
     4861 tccgggcggc ctcggccttg cccgacctgc gcgcggcgac ctcgtgctga gggatgcggt
     4921 ggcgcatctc ggccgggctc cagtactcgg ccttgggcac cgtcgtgtcg ccgaggacga
     4981 tcgcgaagcc gttcatgtcc tgggtcgcga cccgggcgcc gacctggagg ccgagcccgg
     5041 cgacgagcag ccccgccatc aggtacggca tccgccccgc gggtgggctc agcgtccgcc
     5101 cgtcggcgag ggcgatgagg tcggacacac ggcggctgat ccgcctgccc ggtcgcagga
     5161 acgccatggc cccctggtcg acggatcgga gctgggtctt catcaggtcg tacatcttga
     5221 gcagcccgga ggcgacggcc agaggccggg ccgtcagcgt ggcggcccgg cggtcggctt
     5281 cgcgctcacg atcggacgcg agccgccgga aggcgacgtg ggcgagggga ttccaggcga
     5341 cgaggtcgcg caggagcccg gcgacgaaca cgaccctcac gtcgtgggtc tcgaggtgtg
     5401 cgacctcgtg ggcgagcact ccttcgagct cttctcggtc gaggtcgaag atcacggctt
     5461 cggagatc
//
LOCUS       DASQDO010000345         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1199803, whole genome shotgun sequence.
ACCESSION   DASQDO010000345 DASQDO010000000
VERSION     DASQDO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199803"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /gene="ccsA"
                     /locus_tag="VEV43_09675"
     CDS             complement(<1..1315)
                     /gene="ccsA"
                     /locus_tag="VEV43_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYP23831.1"
                     /translation="MRSLAGLGHPSLIAALAFAGIALVLCALGARRSRADLALAGTRA
                     LYATAGFTGLAAVALLNALVLHDFSLEYVVSYSSESLSGGYLVAALWGGMEGSLLFWT
                     VLLTTFSAIAVRSARRNGSRLAGWAGAVLAGITIFFLLLLVAPADPFTTMANVPPDGN
                     GLNPLLQSPGMVVHPPLLYTGFVGFSIPFAFAIAALLAGRSDDSWLRSTRRWTLFAWS
                     ALSIGIVLGGAWAYTELGWGGYWAWDPVENASFMPWLTATAFLHSVVIQEKRRMFKVW
                     NVSLIVLTYVLAVFGTFLTRSGILSSIHTFTEGPIGKWFLPFLALMLVGGLGIVAWRL
                     DSLRGENLFDSLLSRESAFLANNVLFLAAAFTVLWGTIYPIVAEIVSGTRLSVGPPFF
                     NSVFVPIGLALILLTGIGPLISWRRMSRGAFVRVVPVPLPAGAPTA"
     gene            complement(1342..1773)
                     /locus_tag="VEV43_09680"
     CDS             complement(1342..1773)
                     /locus_tag="VEV43_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HYP23832.1"
                     /translation="MQSPTAASPKRRAKFLVGGGIIVFALVGLVVWAMTRPNATSFYY
                     TTSELQAASLAAGAEEYRVNGNVIPESVVNEGLETTFDITDGKTEVTVVTDETLPDAF
                     WTAMDAGSNEVEVVAQGKYDGSHFTATQVLAKCPSKFKAKA"
     gene            1778..2446
                     /locus_tag="VEV43_09685"
     CDS             1778..2446
                     /locus_tag="VEV43_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYP23833.1"
                     /translation="MSKITERPPLRRGPAGSKPRTRAEALRHALGLEVVSLSYNVVEA
                     GIGVAAGIAAGSVALVGFGLDAVVESASASVLIWRLGTERRGRRTAEEAERKAIRLVA
                     VAFGVLALYVGARAVVDLIAGTRPEESTIGIVLAVESLAVMPALARAKRAAARELGSR
                     SLEGDSAQTMLCTYMSAVLLFGLATNALWGWWWADPIAGLVIAALAAREARELWVTED
                     VCCA"
     gene            2443..2904
                     /locus_tag="VEV43_09690"
     CDS             2443..2904
                     /locus_tag="VEV43_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP23834.1"
                     /translation="MSRFEIRPMSQAEAETVAAWHYEPPYDFYDFEADPEDHEEILDP
                     VRRAGDYHSVELDGELAGFFSFRPHGDELELGLGLRPDLSGGGLGLEFVEAGLAFARA
                     AFRPASFWLDVATWNERAKKVYERAGFEAGHVWTHESGQGRVDFLRMTRSA"
     gene            complement(2971..3501)
                     /locus_tag="VEV43_09695"
     CDS             complement(2971..3501)
                     /locus_tag="VEV43_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HYP23835.1"
                     /translation="MTEAAGAAARGRLPSSVLGMALFVSSEVMFFGGLFAAYFMLRGT
                     AAGPWPPEGSVEASLVLPGLLTVVLLASSLTVHAHRLGITIVLGLVFLGGQAYEYSQL
                     GAVGLGASSDVFATLFFTITGFHGLHVAIGVVMLATVWLRGGRMSPGQAEAAAYYWHF
                     VDAVWVLVFLTLYVLR"
     gene            complement(3498..>4103)
                     /locus_tag="VEV43_09700"
     CDS             complement(3498..>4103)
                     /locus_tag="VEV43_09700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYP23836.1"
                     /translation="DELEEYPRTNRRVRLIAFSIALAMVVAALSTLLLRPASRDAPDR
                     LPEFSLEYLSGQGSLTSGELEGSPVVLNFWASWCGPCRDEAPAFQDAWERYDPEGVRF
                     VGVNVCDLEPEAKKFVREFEIGYDVVRDPDQELADALGIECRLPQTFFVAPSGEFAAT
                     SEGEEVDGALGGGGGVVVLGGIEPEELEARIEELLSEEADA"
BASE COUNT          689 a         1428 c         1436 g          550 t
ORIGIN      
        1 gagcggtcgg ggcccccgcg gggagcggga cgggcacgac tctcacgaac gcgccgcggc
       61 tcatccggcg ccacgagatc aacggcccga tgcccgtcaa gaggatgagc gcgagcccga
      121 tcgggacgaa gaccgagttg aagaacgggg gcccgaccga gagccgcgtg ccgctcacga
      181 tctcggcgac gatcgggtag atcgtccccc acagcaccgt gaacgcagcc gcgaggaaca
      241 ggacgttgtt ggcgaggaac gcggactcgc gcgagagcaa cgagtcgaag aggttctcac
      301 cgcgcagaga gtcgagccgc cacgcgacga tccccaaccc cccgacgagc atcagcgcga
      361 ggaacgggag gaaccacttg ccgatggggc cctcggtgaa ggtgtggatg ctcgagagga
      421 tcccggaccg cgtcaggaac gtgccgaaga cggcgaggac gtaggtgagc acgatcaacg
      481 agacgttcca gaccttgaac atccgccgct tctcctggat cacgaccgag tgcaggaacg
      541 cggtcgcggt gagccacggc atgaacgacg cgttctcgac cgggtcccac gcccagtagc
      601 cgccccagcc gagctcggtg tacgcccacg cgcccccgag cacgatcccg atcgacagcg
      661 cgctccacgc gaacagagtc caccgtctcg tcgagcggag ccacgagtcg tccgaccggc
      721 ccgcgagcaa cgcggcgatc gcgaacgcga acgggatcga gaacccgacg aaccccgtgt
      781 agaggagtgg cgggtgcacg accatccccg gtgactgcag gagcgggttg agcccgttcc
      841 cgtccggcgg gacgttcgcc atcgtcgtga aggggtccgc cggcgcgacg agcagcagca
      901 ggaagaagat cgtgatcccg gcgaggacgg ctcccgccca tcccgccagc cgcgacccgt
      961 tgcgccgggc gctgcgcacg gcgatcgccg agaacgtcgt taggagcacg gtccagaaca
     1021 gcaaggaccc ctccatgccc ccccacagcg cggcgacgag ataaccgccg ctcagggact
     1081 cggaggagta gctcacgacg tactcgagcg agaagtcgtg cagcacgagc gcgttcagca
     1141 gcgcgacggc cgcgagcccg gtgaacccgg cggtcgcgta gagggcgcgg gtgccggcga
     1201 gcgcgaggtc ggcccggctc cgtctcgcgc cgagagcaca gaggacgagg gcgatccccg
     1261 cgaacgcgag ggccgcgatc agagaggggt ggccgagccc ggcgagagac cgcatgggtt
     1321 agtgccgctc cgggggagcg attacgcctt cgccttgaac ttcgaggggc acttcgcgag
     1381 gacctgggtg gccgtgaagt gactgccgtc gtacttgccc tgtgccacga cctcgacctc
     1441 gttcgacccg gcgtccatcg cggtccagaa cgcgtccggc agcgtctcgt ccgtgacgac
     1501 cgtgacctcg gtcttcccgt cggtgatgtc gaacgtcgtc tcgagccctt cgttcacgac
     1561 cgactcgggg atcacgttgc cgttcacccg gtactcctcg gcccccgcgg cgagcgaggc
     1621 cgcctgcagc tcggaggtcg tgtaatagaa ggaggtcgcg ttcgggcggg tcatcgccca
     1681 gacgacgaga cctaccaggg caaagacgat gatcccgccc ccgacgagga acttggcgcg
     1741 ccgtttcggc gacgccgcgg tgggagactg catttggatg agcaagatta ccgaacggcc
     1801 acccttgcgc cgggggccgg cgggatcgaa gccccggacc cgggcggagg cgctgcgcca
     1861 cgccctcggg ctcgaggtcg tgagcctctc ctacaacgtc gtcgaggccg ggatcggcgt
     1921 ggccgcgggg atcgccgcgg gctccgtcgc gctggtcggc ttcgggctgg acgccgtggt
     1981 cgagtcggcc tcggcgagcg ttctgatctg gcgcctcggg accgagcgcc ggggccgccg
     2041 gacggccgag gaggccgagc gcaaggcgat ccggctcgtg gcggtggcgt tcggggtgct
     2101 cgcgctctac gtcggcgccc gtgccgtcgt cgacctgata gccgggacga ggcccgagga
     2161 gagcacgatc gggatcgtgc ttgcggtcga gtcgctggcc gtgatgccgg cgctcgcccg
     2221 ggcgaagcgg gccgcggccc gggagctcgg gagccgttcg ctggagggcg actccgccca
     2281 gaccatgctc tgcacctaca tgtcggctgt cctgttgttc ggcctggcga cgaacgctct
     2341 gtggggctgg tggtgggccg acccgatcgc cggcctcgtg atcgcggcgc tggcggcccg
     2401 ggaggcccgt gagctctggg tgaccgagga cgtctgctgc gcttgagccg cttcgagatc
     2461 cggccgatgt cccaggccga ggcggagacg gtggcggcgt ggcactacga gcccccgtac
     2521 gacttctacg acttcgaggc cgacccggag gaccacgagg agatcctcga cccggtcagg
     2581 cgggccggtg actaccactc ggtggagctg gacggggagc tggccggctt cttctcgttc
     2641 cgcccgcacg gcgacgagct ggagctgggg ctcggccttc ggccggacct gtccggcggg
     2701 ggcctgggtc tcgagttcgt ggaggcgggc ctcgccttcg cccgagcggc gttccggccc
     2761 gcgtcgttct ggctggacgt ggcgacgtgg aacgagaggg cgaagaaggt ctacgaacga
     2821 gccggcttcg aggcgggtca cgtatggacg cacgagtcgg gccagggccg ggtggacttc
     2881 ctccgaatga cgcgctccgc gtgaatctgc cgcacccctc gagccccctc ccgttttgtc
     2941 agacaaatgt ctgacaaatt gcgaggctct ctaacggagg acgtacaggg tcagaaagac
     3001 gagcacccac accgcgtcta cgaagtgcca gtagtacgcc gccgcctcgg cttggcccgg
     3061 gctcatccgg ccccctcgga gccacaccgt cgccagcatc accaccccga tcgctacgtg
     3121 gaggccgtgg aagcccgtga tcgtgaagaa gagcgtcgcg aagacgtcgc tcgaggcccc
     3181 caggcccacg gcccccagct gcgaatactc gtaggcctgc ccgcccagga acacgaggcc
     3241 gagcacgatc gtgatgccca atcgatgtgc gtgcaccgtc agcgacgacg ccagcaggac
     3301 cacggtcagc aaacccggca gcaccaacga cgcctcgacc gagccctccg ggggccacgg
     3361 cccggccgcc gtcccgcgca gcatgaagta cgccgcgaac aaccctccga agaacatgac
     3421 ctcggacgac acgaacagcg ccatcccgag caccgacgac gggagccgtc cccgggcggc
     3481 ggcccccgcg gcctccgtca cgcgtcggcc tcctcggaca ggagctcctc tatccgggcc
     3541 tcgagctcct ccggctcgat cccgccgagc acgacgacgc cgccgcctcc gccgagagcg
     3601 ccgtcgacct cctcgccctc gctcgtcgcc gcgaactctc cggacggggc gacgaagaac
     3661 gtctgcggga gcctgcactc gatcccgagc gcgtcggcga gctcctggtc cgggtcgcgc
     3721 acgacgtcgt agccgatctc gaactcgcgc acgaacttct tcgcctccgg ttcgaggtcg
     3781 cacacgttca cgccgacgaa gcggactccc tccgggtcgt atcgctccca cgcgtcctgg
     3841 aacgccggtg cctcgtcgcg gcacggcccg caccacgacg cccagaagtt gaggacgacg
     3901 ggcgagccct ccagctcccc ggaggtgagg gaaccctgcc cggacaggta ctcgagcgag
     3961 aactccggca gccggtcggg cgcgtcgcgc gaggcgggcc tcaacaacag agtgctgagc
     4021 gcggcgacca ccattgcaag tgcgattgag aaggcaatca gccgcactcg ccggttcgtg
     4081 cgcgggtatt cctcgagctc gtc
//
LOCUS       DASQDO010000346         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_649585, whole genome shotgun sequence.
ACCESSION   DASQDO010000346 DASQDO010000000
VERSION     DASQDO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649585"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..640)
                     /locus_tag="VEV43_09705"
     CDS             complement(290..640)
                     /locus_tag="VEV43_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23837.1"
                     /translation="MPNDETEAAATPAPEPMADPGLSGLYGRGTKKKVSLTLDESLLL
                     ELRVLAGNRPLSTAVNDLLEKALAQHRLNELVDELIAEAGEPPPEVYEQILDEWDQGV
                     KQRSTGRHGGASSS"
     gene            704..1849
                     /locus_tag="VEV43_09710"
     CDS             704..1849
                     /locus_tag="VEV43_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HYP23838.1"
                     /translation="MHYLDHAATTPVLPEVADEILRVTTSDFGNPSSVHAAGRRAKAV
                     VEDARDRVAAAVGASPGEIVFTGGGTEADNLAIKGATHKRRGDGNHVVVTAFEHHAVL
                     DAARALTQSGFEVTKVPVEPDGSVDPHRVAEAVRPSTVLVSVMTVNNEIGTIQDLAAI
                     GEAVRAANPRALVHTDAVQALGNLPVDLHAWGVALAAFAAHKAGGPKGVGALFVRAPV
                     AVEPVLHGGGQERGLRSGTHNVAGIAGFGVAAEIAAKEVHEKSERVGALRDRLLDGIR
                     GIVPDVVVNGGLVDRVAGNLNVCIPGTDGETMLLLLDAAGIACSSGSACASGALDPSH
                     VLLALGVPKKLAKGSLRFSLGRTSTDEDVDAVLAALPEVVERARKVA"
     gene            1849..2931
                     /gene="mnmA"
                     /locus_tag="VEV43_09715"
     CDS             1849..2931
                     /gene="mnmA"
                     /locus_tag="VEV43_09715"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYP23839.1"
                     /translation="MPRRKALVAMSGGVDSSVAAALLVDAGYDVTGVMLKLWKGDGAN
                     LGSGCCNLGAAEDARRVADALSIPFYVLNFAEHFEATVRRDFHEEYLAGRTPNPCVRC
                     NHWVKFDALLDKADALGADALATGHYARVDHAGGRYRLRRGVDPTKDQSYVLWMLTQR
                     QLARCLFPVGVQDKATTRAIAEELGLRTAHKPDSQEICFVRDGDFDAYVGESLPGAAV
                     PGPILDVDGEVVGSHRGIGRYTIGQRKGLGIALGRPVFVTSIDAASNAITVGDRGDLA
                     TAEVTLTELSFVSAPPEGRVLVQYRAHGDAYPATIDGTSLRFDVPVEAAAPGQSAALY
                     SIHDPDELVGGGIIQSTVPAIKTPVG"
BASE COUNT          427 a         1135 c         1050 g          465 t
ORIGIN      
        1 ccggcgaggc tggcgacggc gaccacgagg gcgtcgatcg tctgctcgac gcccgacatc
       61 tgcgctgccg tcttgagccc ggcggcctcg cgtgcccccg cttcgtcgac gggaacgact
      121 tcgaaccctt tgataacgcg gttcaccgtg gcgtctctcg ggttcccttg cgtcgactcc
      181 gcgatgacta cggccgggag tacgacgcgt gcttgggctc gtcgcgatag gtcgaggatg
      241 gtgcgcgccc tcgtctcgtt ccgcgccaag gccgacagac cgctgctgtc tacgacgagg
      301 aggcgccacc gtgccggccg gtcgaccgct gcttcacccc ctggtcccac tcgtcgagga
      361 tctgttcgta gacctcgggt ggcggctcac cggcctccgc gatcagctcg tctaccagct
      421 cgttcagccg atgctgcgcg agcgcttttt ccaagaggtc gttgacggcg gtgctcaacg
      481 ggcggttgcc cgcgaggacc cgcagctcta gaagtagcga ttcgtcgagc gtcaatgaca
      541 ccttcttctt ggtcccacgg ccgtagagcc cactgaggcc agggtccgcc atcggttcgg
      601 gcgcgggtgt ggcggccgcc tcagtttcgt cgttcggcat gccgttatcc tacctttggt
      661 aggacagcca atccacggcc cccggccccc gtatcctggg cccatgcact acctcgacca
      721 cgccgccacg acccccgtcc tgcccgaggt cgccgacgag atcctccgcg tcacgacctc
      781 cgacttcggc aatccttcgt ccgtccatgc agccgggcgg cgggccaagg ccgtcgtcga
      841 ggacgcccgc gaccgcgtcg ccgcggccgt cggggcgtcg cccggcgaga tcgtcttcac
      901 cgggggcggc accgaggccg acaacctcgc gatcaagggc gccacccaca agcgcagggg
      961 agacgggaac cacgtcgtcg tcaccgcgtt cgagcaccac gccgttctcg acgccgcccg
     1021 ggccctcacc cagagcggct tcgaggtgac gaaggtcccg gtcgagcccg acggctcggt
     1081 cgatccccac agggtcgccg aggcggtccg gccctcgacc gtcctcgtgt ccgtcatgac
     1141 cgtcaacaac gagatcggca cgatccagga cctcgccgcc atcggcgagg ccgtccgcgc
     1201 cgcgaacccg agggccctcg ttcacaccga cgccgtccag gccctcggca accttcccgt
     1261 cgacctccac gcgtggggcg tcgccctcgc cgcgttcgcc gcgcacaagg ccggagggcc
     1321 gaagggcgtc ggcgcgctgt tcgtgcgcgc gccggtcgcg gtcgagcccg tcctgcacgg
     1381 cgggggacag gagcgcggcc tccgctcggg cacgcacaac gtcgccggca ttgcgggctt
     1441 cggcgtcgcg gccgagatcg cggcgaagga ggtccacgag aagtccgagc gcgtcggcgc
     1501 gttgcgcgac cggctgctcg acggcatccg agggatcgtc ccggacgtcg tcgtgaacgg
     1561 gggcctcgtc gaccgcgtcg ccgggaacct caacgtctgc atccccggca ccgacggcga
     1621 gacgatgctg ctgctgctcg acgcggccgg gatcgcgtgc tcgtccggat ccgcctgcgc
     1681 gtcgggggcg ctagatccct cgcacgtcct gctcgcgctc ggtgtcccca agaagctcgc
     1741 gaaggggagc ctccggttct cgctcgggcg gacgtcgacg gacgaggacg tcgacgcggt
     1801 gctggccgcc ctgccggagg tcgtcgagcg cgctcggaag gtcgcctgat gcctcgtcgc
     1861 aaggcactcg tcgctatgtc gggcggcgtc gactcctcgg tcgcggccgc gctcctcgtc
     1921 gacgccggct acgacgtcac cggcgtgatg ctcaagctct ggaagggcga cggcgcgaac
     1981 ctcggctccg gctgctgcaa cctgggggcc gcggaggacg cccggcgcgt cgccgacgcg
     2041 ctgtcgatcc cgttctacgt gctgaacttc gccgagcact tcgaggcgac ggtgcgccgc
     2101 gacttccacg aggagtacct cgcgggccgc accccgaacc cgtgcgtgcg ttgtaaccac
     2161 tgggtcaagt tcgacgcgct gctcgacaag gccgacgctc tcggagcgga cgccctcgcg
     2221 acgggccact acgcgcgcgt cgaccacgcc ggtggccgtt accggctgcg gcgcggcgtc
     2281 gatccgacca aggaccagtc ctacgtcctg tggatgctga cccagcggca gctcgcacgc
     2341 tgcctgttcc ccgtgggggt ccaggacaag gccacgactc gtgcgatcgc cgaagagctc
     2401 ggcctgagga ccgcgcacaa gcccgactcg caggagatct gcttcgtgcg cgacggggac
     2461 ttcgacgcgt acgtgggcga gagcctgccg ggcgccgcgg tgcccggccc gatactcgac
     2521 gtcgatgggg aggtcgtcgg gagccaccgc gggatcggtc gctacacgat cggccagcgc
     2581 aagggtctcg ggatagcgct cggccgcccc gtcttcgtga cgtcgatcga cgcggcctcg
     2641 aacgcgatca cggtcggcga ccgcggcgac ctcgcgacgg ccgaggtgac gctgaccgag
     2701 ctcagcttcg tctccgcgcc gccggaagga cgcgtgctcg tccagtaccg cgcgcacggc
     2761 gacgcatacc cggcgacgat cgacgggaca tcgctccggt tcgacgtgcc cgtcgaggcc
     2821 gcggcccccg ggcagtccgc cgcgctctac tcgattcacg atcccgacga gctcgtcggc
     2881 ggcgggatca tccagtccac cgttcccgcc atcaagacgc cagttggctg accacgacgc
     2941 gaccgtccgc gccgagttcg accgcgcggc cgcccacttc gccgagcgca ccaagggacg
     3001 gttcgaccac atggatccgg tcgcgttctc gcgcgtctcc gacggcgaca cggtcgtgga
     3061 ggtgggggcc gggacgg
//
LOCUS       DASQDO010000347         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_36986, whole genome shotgun sequence.
ACCESSION   DASQDO010000347 DASQDO010000000
VERSION     DASQDO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36986"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VEV43_09720"
     CDS             <1..462
                     /locus_tag="VEV43_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYP23840.1"
                     /translation="IGFWQFRHQIFYDIGETGSDPFELAIVAAAMFGACAGFLWWNTA
                     PAKIFMGDTGSLMLGGAMAVLAILLNTQLLLIVMGGLYVVETLSVIAQVAIFKRTGKR
                     LFLMAPVHHHFELAGWAEFTVIVRFWVLSGLSVAFGLGLFYADFLSKGGAL"
     gene            459..1814
                     /gene="murD"
                     /locus_tag="VEV43_09725"
     CDS             459..1814
                     /gene="murD"
                     /locus_tag="VEV43_09725"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HYP23841.1"
                     /translation="MKELHGRDVLVVGLGVSGHAAARALLRLGARVRVTEIADTLEMR
                     ERAAELESQGAEVELGAHSFARGDVPLAVVSPGIPPQSEALTALAEQGAEIWGEVELA
                     YRLGDCSFLAVTGTNGKTTTTSLLAQMLTEGGERAVAGGNIGVPLVDAVFEVGRGGVV
                     ALEVSSFQLMTIHEFRAEVAVLLNVAADHLDYHGDLESYAAAKARIVENQRSSDVFVT
                     NLDDPIAMKIAAQAPGRVVTFSASDPGADLTVTGESLTWRGDPFAPLSSLTLEGRGGI
                     EDALAAAGAALEHGVAPAAVARAVESFRPLAHRLQTVARIDGVDYVDDSKATNPHAAL
                     AALRGRRDVVLIAGGRSKGNDLAELRGGVPPVIAVVALGEAAEELRDVFDGVVPVEVV
                     ADMGAAVRAARAHAIPGGSVLLAPACASLDMYTSYAARGDDFARCVREILEVEAANGD
                     A"
BASE COUNT          247 a          665 c          725 g          308 t
ORIGIN      
        1 atcgggttct ggcagttccg gcaccagatc ttctacgaca tcggcgagac cgggtccgac
       61 ccgttcgagc tggcgatcgt cgcggcggcc atgttcggcg cgtgcgcggg ctttttgtgg
      121 tggaacaccg cgcctgcgaa gatcttcatg ggcgacacgg gctcactgat gctcggaggc
      181 gccatggccg tgctcgcgat cctgctgaac acgcagctgc tcctgatcgt gatgggcggc
      241 ctctacgtgg tcgagacgct gtcggtcatc gcacaggtcg cgatcttcaa gcggacgggc
      301 aagcggctgt tcctgatggc ccccgtgcac caccacttcg agctggcggg ctgggccgag
      361 ttcacggtga tcgtccggtt ctgggtgctg tccggtctct ccgtcgcgtt cgggctcggc
      421 ctgttctacg ccgacttcct gtcgaagggc ggggccctgt gaaggagttg cacggccgcg
      481 acgtgctcgt cgtcggcctc ggcgtcagcg gccacgcggc ggcccgggcg ctgttgcgtc
      541 tcggggcgcg cgtgcgcgtg accgagatcg cggacacgct cgagatgcgg gagcgcgcgg
      601 cggagctcga gtcgcagggg gccgaggtcg agctcggcgc tcattcgttc gcgcgcgggg
      661 acgtcccgct cgccgtcgtg agcccgggca tccctccgca gtccgaggcc ctgactgcac
      721 tggccgagca aggtgccgag atctggggcg aggtcgagct cgcttaccgg ctgggcgact
      781 gctcgttcct cgccgtcacg gggacgaacg gcaagacgac cacgacgagc ctcctcgcgc
      841 agatgctgac cgagggtggt gagcgcgccg tcgccggggg gaacatcggc gtcccgctcg
      901 tggatgcggt cttcgaggtg ggccgcggcg gcgtcgtggc gctcgaggtc tcgagcttcc
      961 agctgatgac gatccacgag ttccgggccg aggtcgcggt cctgctgaac gtcgccgccg
     1021 accacctcga ctatcacggc gacctcgaga gctacgcggc cgccaaggcg cgcatcgtcg
     1081 agaaccagag gtcgtcggac gtcttcgtga cgaacctcga cgacccgatc gcgatgaaga
     1141 tcgcggcgca ggcccccggc cgcgtcgtca cgttctcggc gtccgatccc ggcgccgacc
     1201 tgacggtgac gggggagagc ctcacgtggc gcggggaccc gttcgcgccg ctgtcgagcc
     1261 tcaccctgga aggccgcggc gggatcgagg acgcgctggc cgctgcgggg gccgcgctcg
     1321 agcacggcgt cgcccccgct gccgtcgcgc gcgcggtcga gtcgttccgc ccgctggcgc
     1381 accggctcca gaccgtcgcg cggatcgacg gcgtggacta cgtcgacgac tcgaaggcga
     1441 cgaatcccca cgcggccctg gccgcgctgc ggggacgccg cgacgtcgtg ctgatcgccg
     1501 gcggcaggag caaggggaac gacctcgccg agctccgcgg cggcgtccct ccggtgatcg
     1561 ccgtcgtcgc tctcggcgag gcggcggagg agctgcgcga cgtcttcgac ggcgtcgttc
     1621 ctgtcgaggt cgtcgcggac atgggggccg cggtgcgcgc cgctcgcgcc cacgcgatcc
     1681 ccggaggatc cgtcttactg gcgcctgcgt gtgcgagcct ggacatgtac acgagctatg
     1741 cagcgcgcgg ggacgacttc gcgcgctgtg tccgcgagat cctggaggtc gaggcagcaa
     1801 atggcgacgc gtagcggcac gatcggagtc gcccggcggg gctcccgcgc caagccgcgc
     1861 aacgccgccg cggtgcgtcc ggtgtcgtgg tccgacccgg cgggcctgct gatgacgacg
     1921 acgggcgtcc tcgtgctcgt cggcc
//
LOCUS       DASQDO010000348         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_465484, whole genome shotgun sequence.
ACCESSION   DASQDO010000348 DASQDO010000000
VERSION     DASQDO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465484"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VEV43_09730"
     CDS             <1..275
                     /locus_tag="VEV43_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine dehydrogenase"
                     /protein_id="HYP23842.1"
                     /translation="RDLGILYAPDFVINAGGLINVEDELRGYDRERAMKRVEGIYKQL
                     QLIFAMARERKLSTARAAEEHAQERIRKISRIRLVRTAEFGPSSHN"
     gene            280..1428
                     /locus_tag="VEV43_09735"
     CDS             280..1428
                     /locus_tag="VEV43_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYP23843.1"
                     /translation="MSEPLRIIGDGRGLADGAVPEGLGEKDLIELFRWLVLLRTFDER
                     AVALQRQGRIGTYALYWGEEGTQAGALYACEDGDWVYPSYRQNAIGILRGVSVATVLA
                     WWRGYGGVHGFWNPREHRVGPICVPIATHLPHAVGLAWAAKIRRDPIASLSWFGDGAT
                     SEGDFHEAMNFAGVLKAPTVFFCVNNQWAISTPYHKQTATPTIAEKAAAYGMPGIRVD
                     GFDPLACWKATRDALDRARNGGGPTLIEAYCYRLMPHGTADDPRLYRDEAEVERWKHL
                     EPVGRMSQFLARLGVLDSSKEEAIRAEAKETVAEGVREMEAIAQPGRDILFDGVYASG
                     RPYSFDEGLAELAAVERPPEVKPLNAAPKPGSTGDHVDPPANPDQERA"
     gene            1425..2414
                     /locus_tag="VEV43_09740"
     CDS             1425..2414
                     /locus_tag="VEV43_09740"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYP23844.1"
                     /translation="MSGLTMVDALNRALREAMSADDRVVVLGEDVAQTGGVFRVTSGL
                     LKEFGEERVVDTPLAEAGIVGSAIGMAMYGLLPVVEIQFDVFVYPGFEQIVVHAARLA
                     WRTKGEVPASIVVRIPYGGGVRAPELHSDSPEGLFTHVPGLKVVCPTTPSDARAMMLA
                     AIQDPDPVIFMEPKRIYRAGREDVPENGHGDYSEYLSKARVARDGDDLAIITYGATVP
                     LSLQAAERLESERGVRARVLDLRSLYPLDVDAIVGAASECGRALVVHEAPRMNGIGAE
                     VSSLIHERAMLDLEAPVGRVTGYDVPFPQFANEDDYLPSVDRIFLGATKVLDF"
     gene            2414..3598
                     /locus_tag="VEV43_09745"
     CDS             2414..3598
                     /locus_tag="VEV43_09745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYP23845.1"
                     /translation="MPDFKLPDLGEGVTEAEVDRWLVSEGDVVGEDDPLVELITDKAT
                     AEIPSPYEGVVSRIHVKAGEVVPVGTVLITIGDGSAPAAAPPPPPPPARAEAPARAEA
                     PAPEPRSSNGSVKAMPPVRKLARDLGVDLTSVRGSGPGGRIVREDVEAAAGRSAPVEA
                     APSGGRREPLRGVRRLIAERMSAAHRIVPPVTHVEECDVTELDATRKLVNERNPDELR
                     LTYMPFIVKAVVAGLKQYPALNASLDEDAGEIVFHDRYDIGIAVDTPAGLVVPVVRDA
                     DRKRLREVAADVDRLARGAREGTLRSDDLKGGTFTISSPGPFGGLMATPIVFHPQSAI
                     LGVHRASERPVVRDGQIVARQMMNLSITFDHRILDGMTAAKFVLDVAKLLEHPAALAL
                     EA"
     gene            complement(3827..4000)
                     /locus_tag="VEV43_09750"
     CDS             complement(3827..4000)
                     /locus_tag="VEV43_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23846.1"
                     /translation="MRRKMKLALVAGAMAAMAVPAAPAQASICTSDPAVICQVYYLAT
                     RTICKVTGGPCAT"
BASE COUNT          606 a         1391 c         1489 g          620 t
ORIGIN      
        1 tgcgcgacct cggcatcctc tacgcccccg acttcgtcat caacgccggt gggctcatca
       61 acgtcgagga cgagctgcgc ggctacgacc gcgaacgtgc gatgaagcgg gtggagggca
      121 tctacaagca actccagctg atcttcgcca tggcgcgcga gcgcaagctg tccaccgccc
      181 gcgccgccga ggagcacgcc caggagagga tccgcaagat cagccggatc cgcctcgtgc
      241 gcacggcgga gttcggcccc agctcgcaca actgatgccg tgagcgaacc cctacggatc
      301 atcggcgacg gccgcgggct ggcggacggc gccgtcccgg aaggcctggg cgagaaggac
      361 ctcatcgagc tcttccgctg gctcgtgctc ctgcgcacgt ttgacgagcg cgcggtcgcg
      421 ctgcagaggc agggacgcat cgggacgtac gcgctgtact ggggcgagga ggggacgcag
      481 gccggagcgc tgtacgcgtg cgaggacggc gactgggtct atccgtccta ccggcagaac
      541 gcgatcggga tcctccgcgg cgtctcggtc gcgaccgtgc ttgcgtggtg gcgcggctac
      601 ggcggggtgc acggtttctg gaacccgcgc gagcaccgcg tcggcccgat ctgcgtcccg
      661 atcgcgacgc acctgccgca cgccgtcggc ctcgcgtggg cggcgaagat ccgccgcgac
      721 ccgatcgcct cgctgtcgtg gttcggcgac ggcgcgacct ccgaaggcga cttccacgag
      781 gcgatgaact tcgccggcgt gctgaaggcg ccgacggtct tcttctgcgt gaacaaccag
      841 tgggcgatct cgacgccgta ccacaagcag accgcgacgc cgacgatcgc cgagaaggcc
      901 gccgcttacg ggatgccggg catccgcgtc gacgggttcg acccgctcgc gtgctggaag
      961 gcgacgcgcg acgcgctcga ccgggcgcgc aacggcgggg gcccgactct catcgaggcg
     1021 tactgctacc ggctgatgcc gcacgggacc gccgacgacc cgcgcctgta ccgcgacgag
     1081 gccgaggtcg agcggtggaa gcacctcgag cccgtcggtc gcatgtcgca gttcctcgcg
     1141 cggctcggcg tgctcgattc ctccaaggaa gaggccatca gggccgaggc caaggagacc
     1201 gttgccgagg gcgtgcgcga gatggaggca attgcgcagc ccggccgcga catcctgttc
     1261 gacggcgtct acgcgagcgg acggccgtac agcttcgacg agggcctggc cgagctcgcg
     1321 gccgtcgagc gaccgcccga ggtcaagcct ctcaacgcgg cgcccaagcc cggctccacc
     1381 ggtgaccacg tagacccacc ggcgaatccg gaccaggagc gagcatgagc gggctgacga
     1441 tggtcgacgc gctcaaccgg gcgctgcgcg aggcgatgtc tgcggacgac cgcgtggtcg
     1501 tgctcggcga ggacgtcgcg cagaccggcg gtgtcttccg ggtgacgtcg gggttgctga
     1561 aggagttcgg cgaggaacgc gtggtcgaca cgccgctcgc cgaggccggc atcgtcggct
     1621 ccgcgatcgg gatggcgatg tacgggttgc tgccggtcgt cgagatccag ttcgacgtgt
     1681 tcgtctaccc ggggttcgag cagatcgtgg tgcacgccgc gcggctcgcg tggcgcacca
     1741 agggtgaggt ccccgcgtcg atcgtcgttc gcatcccgta cggcggaggc gtgcgtgctc
     1801 cggagctgca ttcggactcg cccgagggct tgttcacgca cgtgccgggc ctcaaggtcg
     1861 tgtgcccgac gacgccgtcg gacgcgcggg cgatgatgct cgccgcgatc caggacccgg
     1921 accccgtgat cttcatggag cccaagcgca tctaccgtgc gggccgcgag gacgtccccg
     1981 agaacggcca cggcgactac tccgagtacc tgtcgaaggc gcgcgtcgct cgcgacggtg
     2041 acgacctggc gatcatcact tacggcgcga cggtgccgct gtcgctgcag gccgccgagc
     2101 ggttggagtc ggaacgcggt gtgcgcgccc gggtcctcga ccttcgctcg ctgtatccct
     2161 tggacgtcga cgcgatcgtc ggcgcggcat ccgagtgcgg acgcgctctg gtcgtgcacg
     2221 aggcgccgcg catgaacggg atcggggccg aggtgtcgtc gctgatccac gaacgggcga
     2281 tgctcgacct cgaggccccc gtgggccgcg tgaccggcta cgatgtcccg ttcccgcagt
     2341 tcgcgaacga ggacgactac ctcccgtcgg tcgaccggat cttcctcggc gcgaccaagg
     2401 tcctggactt ctagtgcccg acttcaagct ccccgacctc ggggagggcg tgaccgaggc
     2461 cgaggtcgac cgctggctcg tctccgaggg cgacgtcgtc ggcgaggacg acccgctcgt
     2521 cgagctgatc accgacaagg cgacggccga gatcccgtcg ccgtacgagg gtgtcgtgtc
     2581 gcgcatccac gtgaaggccg gcgaggtggt gcccgtcggg accgtgctga tcacgatcgg
     2641 cgacggctcg gcccccgctg cggcacctcc gcctccgcct ccgccggctc gtgccgaggc
     2701 accggctcgt gccgaggcgc cggcccccga gccccgctcg tcgaacggct cggtgaaggc
     2761 gatgccgccg gtacgcaagt tggctcggga cctgggcgtg gacctgacgt cggtgcgggg
     2821 atcggggccg ggtggacgga tcgtgcgcga ggacgtggag gccgcggcgg ggcgttcggc
     2881 ccctgtcgaa gcggcgccgt cgggtggtcg ccgcgagccg ttgcgcggcg tgcgtcggtt
     2941 gatcgccgag cgcatgtcgg cggcgcaccg gatcgtgccg cccgtcacgc acgtcgagga
     3001 gtgcgacgtc accgagctcg acgcgacgcg caagctcgtg aacgagcgga acccggacga
     3061 gctccggctc acctacatgc cgttcatcgt gaaggcggtc gtggcggggc tgaagcagta
     3121 cccggcgctc aacgcgtcgc tcgacgagga cgcgggcgag atcgtgttcc acgaccgcta
     3181 cgacataggg atcgcggtag acacgccggc gggcttggta gtgccggtgg tgcgggacgc
     3241 ggaccgcaag cgcctgcgcg aggtggctgc ggacgtcgac cgacttgctc ggggcgcacg
     3301 cgagggaacg ctgcggtcgg acgacctgaa gggcgggacg ttcacgatct cgagtcccgg
     3361 cccgttcggg ggcctgatgg cgacgccgat cgtgttccac ccgcagtcgg cgatcctggg
     3421 cgtgcaccgg gcctcggagc gtccggtggt tcgggacggc cagatagtcg cccggcagat
     3481 gatgaacctg tcgataacgt tcgaccaccg gatcctggac ggcatgacgg cggcgaagtt
     3541 cgtgctggac gtagcgaagc tgctggagca cccggccgcg ctggccctgg aggcctagga
     3601 gggctccggt tcgcctacga tgagccgatg ggcatcgcgt ccgcaattct gcagtcactc
     3661 ctgtacgtag ccgcggtggc ggctgcgggc gctgtggccg ggctcgctgc ggcgggggtg
     3721 cgcaagctca tcgacttccg tttccgtcgc gcatagcgcg acacaagcga cactcgacga
     3781 aaaggagccc ctcgcggggc tccttttcac tccttacggc cggctatcag gtggcgcagg
     3841 ggccgcccgt taccttgcag atcgtccgcg tcgccaggta gtagacctgg cagatcacgg
     3901 ccgggtcgct ggtgcagatc gatgcctgtg ccggggccgc cggaaccgcc atcgccgcca
     3961 ttgcccccgc taccagcgcg agcttcatct tccgccgcat cgtgcctcct cgttcgcccg
     4021 acttgccggg tggaatctag caagccgagc ggttctaggc caggcagccc aggtacttcc
     4081 cgaggacctt gcactccacg tagccg
//
LOCUS       DASQDO010000349         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_526404, whole genome shotgun sequence.
ACCESSION   DASQDO010000349 DASQDO010000000
VERSION     DASQDO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526404"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VEV43_09755"
     CDS             <1..384
                     /locus_tag="VEV43_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23847.1"
                     /translation="ERALLELGVLFALVISLAAGLVAGLDAIWERRREMVILLSAGIA
                     PKVLRRAQALFLSIPLTVSCFLGAGTGALVDAALDTGAQVPIGVAEDLGLSLVLVVAT
                     SFLFASVVARWAISMRTLRDAVRTP"
     gene            complement(397..870)
                     /locus_tag="VEV43_09760"
     CDS             complement(397..870)
                     /locus_tag="VEV43_09760"
                     /inference="COORDINATES: protein motif:HMM:NF022736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 family protein"
                     /protein_id="HYP23848.1"
                     /translation="MTPGAVFEEHQRPPRGYVVTGGVALAFAAGVALWFSALAGSLQW
                     DLLAFLALGAFVVGFTQMRTVVTTTQLRVSIVPFPRKTVALADVETCEPETYRPLLHY
                     GGWGWKWSPSRGWAYTMRGNRGVRIRLRNGKTFLIGSQRPDELMAAIRSSTRAVR"
     gene            complement(875..1792)
                     /locus_tag="VEV43_09765"
     CDS             complement(875..1792)
                     /locus_tag="VEV43_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYP23849.1"
                     /translation="MPRPFRFAIQLRSAGSRDEWLGRARRAEALGYDVVCVPDHAGTQ
                     LSPFVALTAVAAATDRIRVGTFVLDNDFRHPVLAAHEAATVHLLSGGRFDLGLGAGWL
                     RSDYDALGIEFAPPRTRVERLEEAVEIVDLYFRGETFSFSGRHYSVEEVEPLRLQPGM
                     TRPPLVIGGGGRRVLALAGRRADVASVFLKAKPEGSTFEPASPEEYATKVAHVRRSAG
                     DRDVEINVLLQAFDVTDDPGAAFARWADDFETTPEELRALPFCLAGTTDEIVDDLQRR
                     REEYGISYVTVFGEHLETFAPVVERLAGR"
     gene            complement(1812..3674)
                     /locus_tag="VEV43_09770"
     CDS             complement(1812..3674)
                     /locus_tag="VEV43_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23850.1"
                     /translation="MADNPTRPARLCAALLVLVALVAAPASSNATPQRTADPLAEWVE
                     EAPDRFFYWAQRAVRPELAEAFQGLDPGGRFILNFPWGDMTGDRSDDVLTIEIDSGPG
                     GLGLAGYSTTFRALNGGNGNPLWSRTFESEVVFPIEVTLGKKARTGVLVLSYDFEEDA
                     TTFLALDHRGRRAYRHTFVASTQTDEGYVTGREEVISVALQSSLRGRATDVLIGVADV
                     RRIPEVHPEVPSVAGVTSTLVIDGRTGRRVEHSEAELGIGRVPVPVRAPDLDGDGLED
                     HLMTYVLPEAGSDEESGLPTLPSFEGELVRARRGTDGTRLWTSPALELDDGWDAPPLV
                     THVTLGDQTRDGNDEVLLGFDRDPYITPSFDLSYPGDRKGVWSLNGRDGDVLWHRGVS
                     SAVVLDDLDGDRRRDVLLVDDVSSRKSGTRVTGASGLDARREWSRFFRVRTDDDQTVE
                     SSLGRAGDLQPDGVSDFVLVQMLRRDFENGTEWRWAGERLFSGASGKGLGEIRTVWPL
                     GASVDGRGDDLFRWADDGFEILDGATRRQRLAIGLEIPLTLPTDGSALLTFPARLGRD
                     RCADFVGTILSSTSTFAVGIDGGSGRLLWAKRQQGIDVGGPVTQTRRVDRNRAC"
     gene            3783..>4678
                     /locus_tag="VEV43_09775"
     CDS             3783..>4678
                     /locus_tag="VEV43_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23851.1"
                     /translation="MARRAGMLAAVAVVVLGGVVTASAQEGPACHGQAATHVGTRGPD
                     VITGTRGDDVVVARGGNDRIETLGGDDTICAGRGRDVVRAGKGFQDTVYAGGGDDVVH
                     GGPGRNFLVAGPGDDRMNGGSHFDSFESGRRGDRDDEDLFVGRARSDSFHGDPGDDVI
                     RGGDGTDRIWYFESPAGVTVDLLKGTATGDGRDTLASIEDVVGTRHDDFVRATNGDDS
                     VFTSTGDDVVYGLGGDDDITDSGGRDSLFGGPGRDLVASSGCVSSSDGPTRCQTDAPK
                     PDVLKGGAGPDTVGSGPGEDRV"
BASE COUNT          692 a         1658 c         1641 g          687 t
ORIGIN      
        1 gagagagccc tgctcgagct cggcgtccta ttcgcgctcg tgatctcgct cgccgcgggc
       61 ctcgtcgccg ggctcgacgc gatctgggaa cgacgtcgcg agatggtgat cctgctcagc
      121 gccgggatcg ctcccaaggt cctgcgacgc gcgcaggccc tctttctctc cataccgctg
      181 acggtgtcct gcttcctcgg ggcggggacg ggcgccctgg tcgacgcggc cctcgatacg
      241 ggcgcgcagg tgccgatcgg cgtcgcggaa gatctcgggc tgagcctcgt gttggtcgtc
      301 gcgacgagct tcctcttcgc ctcggtcgtc gcccggtggg caatctcgat gaggacgctt
      361 cgtgacgccg ttcggacgcc gtgaccggac gaacgtctag cgcaccgccc gcgtagagct
      421 ccggatagcc gccatgagct cgtcgggccg ctgcgagccg atcaggaagg tcttgccgtt
      481 gcgcaggcgg atcctgacgc ctcggttccc gcgcatcgtg tacgcccagc cccgcgacgg
      541 gctccacttc cagccccagc ccccgtagtg gagcaggggc cggtaggtct ccggctcgca
      601 cgtttcgacg tcggccagcg caaccgtctt gcgcgggaag gggacgatcg acacgcgtag
      661 ttgcgtcgtc gtcacgaccg tcctcatctg cgtgaacccg acgacgaacg cgccgagcgc
      721 gaggaacgcc aagaggtccc attggagcga cccggcgagc gccgagaacc acaacgccac
      781 gccggcggca aacgcgagcg cgaccccgcc cgtaaccacg tatccccgcg ggggcctctg
      841 gtgctcctcg aagacggcgc ccggtgtcat tggctcatct tcccgccagc cgctccacta
      901 cgggagcgaa cgtctcgagg tgctcgccga agaccgtgac gtagctgatc ccgtactcct
      961 cccgacggcg ctgcaggtcg tcgacgatct cgtccgtcgt ccccgcgagg cagaacggca
     1021 gcgcccgaag ctcctccggc gtcgtctcga agtcgtccgc ccaacgggcg aacgcggccc
     1081 ccggatcgtc cgtgacgtcg aacgcctgga gcaggacgtt gatctcgacg tcgcggtccc
     1141 ccgcgctccg gcgaacgtgc gcgaccttgg tcgcgtactc ctccggcgac gcgggctcga
     1201 acgtcgaccc ttcaggtttg gccttcagga acacgctcgc gacgtccgcc cggcggcccg
     1261 ccagcgccag cacccgccgt cccccaccgc cgatcaccag aggcggccgc gtcatcccgg
     1321 gctggaggcg caggggctcg acctcctcga ccgagtagtg gcgacccgag aaggagaagg
     1381 tttcaccacg gaagtacagg tcgacgatct cgactgcttc ctcgagccgc tcgacgcggg
     1441 tccgcggcgg cgcgaactcg atcccgagcg cgtcgtagtc gctccggagc caccctgcgc
     1501 cgaggccgag gtcgaacctt ccccccgaaa gcaggtggac ggtcgcggcc tcgtgcgcgg
     1561 cgagcaccgg gtgccggaag tcgttgtcga gaacgaacgt cccgacgcgg atccggtcgg
     1621 ttgcggccgc gaccgcggtg agcgcgacga acggagacag ctgtgtgccg gcgtggtcgg
     1681 gaacgcacac gacgtcgtag ccgagggcct cggcgcggcg cgcgcgtccg agccattcgt
     1741 cgcgcgagcc cgcggagcgg agctggatcg cgaatcggaa cgggcggggc atccgccgaa
     1801 catacgcggc gtcagcaggc tcggttgcgg tcgacccttc gggtctgcgt caccgggccc
     1861 ccgacgtcga tcccctgctg acgcttcgcc cacaggagcc ggccggaacc gccgtcgatg
     1921 ccgactgcga acgtgctagt cgagctcagg atcgtcccga cgaagtccgc gcagcgatcg
     1981 cggcccaaac gcgccgggaa cgtgagcagc gcgctgccgt cggtcgggag cgtgagcggg
     2041 atctcgagcc cgatcgcgag tcgctgccgg cgcgtcgccc cgtcgaggat ctcgaacccg
     2101 tcgtcggccc agcggaacag gtcgtctccg cgtccgtcga ccgaagcccc caggggccag
     2161 acggttcgga tctcgccgag tcccttgccg gacgctcccg agaagaggcg ctcgccggcc
     2221 cacctccact ccgtcccgtt ctcgaagtcg cgcctgagca tctggacgag cacgaagtcc
     2281 gagacgccgt cgggctgtag gtcaccggct ctgccgaggc tcgactcgac cgtctgatcg
     2341 tcgtccgtgc ggacacggaa gaagcgcgac cactcccggc gggcatccag cccggacgca
     2401 ccggtgaccc tcgtccccga cttgcgcgag ctcacgtcgt ccacgagcag gacgtctcgt
     2461 cgccggtctc cgtcgaggtc gtcgagcacg acggcggagc tgacgccgcg gtgccacagc
     2521 acgtcgccgt cccgaccgtt gagcgaccac acgcccttgc ggtcgccggg atacgagagg
     2581 tcgaagctgg gagtgatgta cgggtcacgg tcgaagccga gcaggacctc gtcgttgccg
     2641 tcgcgcgtct ggtcgcccaa cgtgacgtgc gtaacgagcg gcggcgcgtc ccatccgtcg
     2701 tcgagctcga gagccggcga ggtccacagc ctcgtcccgt ccgtgccgcg gcgtgcgcgc
     2761 acgagctctc cctcgaacga aggaagcgtc ggaagaccgc tctcctcgtc cgagcccgcc
     2821 tccggcagca cgtacgtcat caggtggtct tcgagcccgt caccgtcgag atcgggggcg
     2881 cgcaccggga cgggcacccg cccgatgccg agctcggcct ccgagtgctc gacgcgtctc
     2941 cccgtgcggc cgtcgatcac gagcgtgctc gtcacgcccg ctacggacgg gacctcgggg
     3001 tggacctccg ggatccgcct cacgtccgcg accccgatca acacgtcggt agcgcggccg
     3061 cgcagcgagc tctgtagcgc gaccgagatc acctcctcgc ggcccgtcac gtagccctcg
     3121 tctgtctgcg tcgacgctac gaacgtgtgc cggtacgcgc ggcggccccg gtgatccagc
     3181 gcgaggaacg tcgtcgcgtc ctcttcgaag tcgtacgaga ggacgagcac gcccgtccgc
     3241 gctttcttcc cgagggtgac ctcgatcggg aagacgacct cggactcgaa ggtccggctc
     3301 cacagcgggt tgccgttgcc gccgttcagg gccctgaacg tcgtcgagta gccggcgagc
     3361 ccaagccctc cgggcccgga gtcgatctcg atcgtcagaa cgtcatcgct acgatcgccc
     3421 gtcatgtcgc cccacgggaa gttgaggatg aacctcccgc cgggatcgag cccctggaag
     3481 gcctcggcga gctcgggccg gactgcccgt tgggcccagt agaagaaccg atcgggcgcc
     3541 tcttcgaccc attccgcgag gggatcggcc gtgcgttgcg gcgtcgcgtt cgagctggcg
     3601 ggcgcggcca cgagcgcgac gaggacgagc agcgccgcgc agagccgagc cggccgggtg
     3661 gggttgtccg ccatggagac ctcctagtcc gtcgctaagc tttcggggtc ccgggctcgg
     3721 tctcctgccg gaaccggtga cgagtcggtg tcagaggcgg ctccgtgggt acggtcgccg
     3781 caatggctcg acgtgccgga atgctcgccg cggtcgccgt ggtcgtcctc gggggcgtcg
     3841 tcaccgcttc cgcccaggaa ggtccggctt gtcacggaca agccgccacc cacgtcggga
     3901 cccgggggcc agacgtgatc acggggacgc ggggcgacga cgtcgtcgtc gcacgcggag
     3961 gcaacgacag gatcgagact ctcgggggcg acgacacgat ctgcgcgggc cgcggacgcg
     4021 acgtcgtgcg cgcgggcaag ggcttccagg acaccgtgta cgcaggtggg ggcgacgacg
     4081 tcgtccacgg ggggcccggc cggaatttcc tcgtcgcggg gcccggcgac gatcgcatga
     4141 acgggggctc gcacttcgac agcttcgagt cgggccgccg cggcgaccgc gacgacgagg
     4201 atctgttcgt cggcagggcg cggagcgact cgttccacgg cgacccgggg gacgacgtga
     4261 tccgtggtgg cgacggcacc gaccggatct ggtacttcga gtcgcccgcg ggtgtgacgg
     4321 tcgacctcct gaaggggacg gctaccggag acggacgcga cacgctcgcg tcgatcgagg
     4381 acgtcgtcgg cacgcggcat gacgacttcg tccgcgccac caacggggac gactcggtct
     4441 tcacgagcac gggtgacgac gtcgtgtacg gcctcggcgg agacgacgac atcaccgatt
     4501 cgggagggcg cgactcgctg ttcggggggc cgggtcgcga cctcgtcgcg tcgagtgggt
     4561 gcgtctcgtc gagcgacggg ccgacgcggt gtcagaccga cgcgccgaag cccgacgtgc
     4621 tgaagggtgg tgccggtccg gacacggtcg gctcggggcc cggtgaggac cgcgtctt
//
LOCUS       DASQDO010000350         4308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_588305, whole genome shotgun sequence.
ACCESSION   DASQDO010000350 DASQDO010000000
VERSION     DASQDO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588305"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..694)
                     /locus_tag="VEV43_09780"
     CDS             complement(14..694)
                     /locus_tag="VEV43_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HYP23852.1"
                     /translation="MAPTNPNLSWQPTLFDTVAPSFDARFSTLERIELDPTAWVDYAP
                     AWVEGSDELFAQVLEERKWGQRSRRMYENRVVEPRLTAPWNARSGEELRPPVLEEIRI
                     ALSEQYGIEFDSVGFNLYRDGSDSVAWHGDHIKKEIEEPLVALVSLGEPRRFLLRPRE
                     GGKSRAFMLGRGDLLVTGGKTQRTWEHSVPKVAQAGPRISLAYRHGLDPRAYAHKRNV
                     EDDEGRPS"
     gene            complement(705..1178)
                     /locus_tag="VEV43_09785"
     CDS             complement(705..1178)
                     /locus_tag="VEV43_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HYP23853.1"
                     /translation="MTNEKRHKHPPPADHAHAYEVVEGLFISGHPDNADDFMRRGVDA
                     VIDLEGDVDTAIPEEEGQRGTTLYVYWPIEDKEEMPEEDVVRSISSFVSTLLDRDKEV
                     LVHCRSGHNRSGLVCARTLIERGMEPDEAIEKVRSGRSDGHALTNQTFVDWLKRE"
     gene            1313..2125
                     /locus_tag="VEV43_09790"
     CDS             1313..2125
                     /locus_tag="VEV43_09790"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HYP23854.1"
                     /translation="MKTEHRDHPLGTPGLSARQMARSLEPSSRVLRFLATLFPGLRGF
                     GSSWEKGAKGEEAVAKKLAELPAERWNAVHDRPLGEGRRNVDHVVVGRGGVFSVNAKN
                     LSGKVVVKRNAFLVNNRADKCLHVARDEAVRVARLLTSATGEPVAVTPVIVVRCAALD
                     IQAQPEGVHVISVHDVPQWFEAHGEVLDAPRVSRIWRSVCRPGLWTSPVPPVVTGDLS
                     TKVWKRYGQDRVYVNDGAGKRLGHLNRKTGEIHVDVAARREDVRAVLAEYVQ"
     gene            complement(2138..2563)
                     /locus_tag="VEV43_09795"
     CDS             complement(2138..2563)
                     /locus_tag="VEV43_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYP23855.1"
                     /translation="MIDLIAESLDEQELAEMYATDDPSVRPRSSWPAWKDEGSDAAAC
                     YFEIPPGCSLGPHVHDAEESVVILAGRATATIGGERKALEGPALVVMPEGVTHDLTNE
                     GDDTLRAVGYFPRNSVTTTFEQEMQPNGSRTAGTPDSAA"
     gene            2659..2919
                     /locus_tag="VEV43_09800"
     CDS             2659..2919
                     /locus_tag="VEV43_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23856.1"
                     /translation="MGTRRRLACALLALVAALSTGVVPAGAHNKGKHCKKGYKPCLHP
                     ASDYDCAGGSGDGPKYVYGTVKVTGSDPYGLDSDNDGIGCED"
     gene            complement(2975..3946)
                     /locus_tag="VEV43_09805"
     CDS             complement(2975..3946)
                     /locus_tag="VEV43_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYP23857.1"
                     /translation="MDYRHLGRSGLEVSRLCLGTMNFGPQTSEDDSHAIMDRALELGF
                     NFFDTANVYGWKKGEGITEQIVGRWFAQGGGRREKTVIATKLYGSMSDWPNDTFLSAR
                     NIRHACEASLRRLQTDYIDLYQMHHVDRNTPWDEIWEAMEVLRAQGKILYVGSSNFAG
                     WHIAKAQGAAQRRNFTGLVSEQSIYNLATRAVELEVLPACLDFGVGVIPWSPLHGGLL
                     GGVLRKEREGKRRHTGRALDALEARRDQIQAYEDFCGEIGEDPATVALAWLLHQPAVT
                     APIVGPRTMEQLDDSMRALKLTLGADELGRLDEIWPGHKTAPEDYAW"
     gene            complement(3980..>4308)
                     /locus_tag="VEV43_09810"
     CDS             complement(3980..>4308)
                     /locus_tag="VEV43_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23858.1"
                     /translation="ALQALDAGELDPHKGEIVERTWDEDMLAGDVTWLLSTPFGRLDL
                     VFEPAGTGGYDDLARDGIDVDLGGIRARVASLRDVIRSKEALNRPRDREQLPTLRRLL
                     DLAEGE"
BASE COUNT          643 a         1452 c         1476 g          737 t
ORIGIN      
        1 agaaggccgc cccttacgag gggcggcctt cgtcgtcttc gacgttcctc ttgtgcgcgt
       61 aggcgcgggg gtcgaggccg tgccggtacg cgagcgagat gcgcggcccc gcctgcgcga
      121 ccttcggcac gctgtgctcc cacgttcgct gagtcttgcc gccggtgacg agaaggtcgc
      181 cccggccgag catgaacgcg cgcgacttgc cgccttccct gggccggagg aggaagcgac
      241 gcggctcccc gagcgacacc agcgcgacga gcggctcctc gatctccttc tttatgtggt
      301 ccccgtgcca ggcgacgctg tcgcttccgt cccggtagag gttgaacccg acggagtcga
      361 actcgatgcc gtactgctcg gagagcgcga tgcgtatctc ctcgaggacc gggggtctca
      421 gctcctcacc ggagcgcgcg ttccacggcg cggtaaggcg cggctcgacg acgcggttct
      481 cgtacatgcg acgtgaccgc tggccccact tgcgctcctc cagcacctgc gcgaacagct
      541 cgtcggagcc ctcgacccac gcgggcgcgt agtccaccca cgcggtcggg tcgagctcga
      601 tgcgttcgag cgtcgagaac cgcgcgtcga agctcggcgc gaccgtgtcg aagagcgtgg
      661 gctgccagga gaggttcggg ttcgtcggcg ccatgttcgt tcgattattc gcgcttcagc
      721 cagtcgacga acgtctggtt cgtgagggca tggccgtcgg agcgccccga ccggaccttc
      781 tcgatggcct cgtccggctc catgccgcgc tcgatcagcg tccgtgcgca gacgaggccg
      841 gagcggttgt ggcccgagcg gcagtggacg aggacctcct tgtcgcggtc gagcagcgtc
      901 gagacgaacg acgagatcga ccggacgacg tcttcctcgg gcatctcctc tttgtcctcg
      961 atcggccagt agacgtagag ggtggtgccg cgctggccct cctcctcggg gatggccgtg
     1021 tcgacgtcgc cctcgaggtc gatcacggcg tcgacgccgc ggcgcatgaa gtcgtcggcg
     1081 ttgtcggggt ggccggagat gaagaggccc tcgacgacct cgtaggcgtg ggcatgatcg
     1141 gcggggggcg ggtgcttgtg tcgcttctcg ttagtcatga tggatgttgt tctccgccgt
     1201 gcgagacggg caaacgggcg gaacggaggt gggccgggcg attagccgct tcggttacga
     1261 aaagtacatc cggcgctaaa gaccgcgggg ccgggagtcg acccaaagac ccatgaagac
     1321 cgagcacagg gatcaccccc taggcacgcc gggcctctcg gcccgccaga tggctcgttc
     1381 actcgagccg tcgtctcgcg tcttgcgctt cctagcgacc ctcttccccg gcctccgggg
     1441 gttcgggtct tcctgggaga agggggccaa aggcgaggag gccgtcgcca agaagctcgc
     1501 cgagctgccc gccgagcggt ggaacgcagt tcacgatcgt ccgctcgggg agggccggcg
     1561 caacgtggat cacgtcgtgg tcgggcgcgg gggcgtcttc agcgtcaacg cgaagaacct
     1621 cagcggcaag gtcgtcgtca agcgcaacgc cttcctcgtg aacaaccgcg ccgacaaatg
     1681 ccttcacgta gcgcgggacg aggcggtgcg cgtcgctcgg ctgctgacgt cggcaacggg
     1741 tgagcccgtg gccgtcacgc cggtgatcgt cgttcggtgc gcggccctcg acatacaggc
     1801 tcagcccgag ggtgtccacg tcatatccgt gcacgacgtc ccgcagtggt tcgaagcgca
     1861 cggcgaagtg ttggacgcgc cccgcgtgtc tcgcatctgg cgatctgtct gccggccggg
     1921 cctctggacg tcaccggtcc ctcccgtcgt gacgggcgac ctgtcgacga aagtctggaa
     1981 gcgctacggg caggaccgcg tctacgtcaa cgacggggcc gggaaaaggc tcggccacct
     2041 caacaggaag acgggcgaga tccacgtcga cgtcgcggcg cggcgcgagg acgtgcgggc
     2101 cgtgctcgcc gaatacgtcc agtagcgaag cgcccggcta ggccgcgctg tccggggtgc
     2161 ctgccgtccg gctcccgttc ggctgcatct cttgctcgaa cgtcgtcgtc accgagtttc
     2221 tcgggaagta gccgaccgct cgtagcgtgt cgtcgccttc gttcgtcagg tcgtgcgtga
     2281 caccttccgg catcactacc agcgccgggc cctccagggc cttgcgctcg cccccgatcg
     2341 tcgccgtcgc ccggcccgcc aggatcacga ccgactcttc cgcgtcgtgg acgtgggggc
     2401 cgaggctgca gcccggcggg atctcgaagt agcacgcggc ggcgtccgag ccttcgtcct
     2461 tccatgccgg ccagctgctg cgaggcctca ccgacggatc gtcggtcgcg tacatctcgg
     2521 cgagctcttg ttcgtccagc gactctgcga tcaagtcgat cacctccggc tcgtatgcct
     2581 tctttgcgta tgtgctcgcc gtgaccaccc gcctatcgtc gtgtcacatc acgaccgaca
     2641 aacgaggggg acgggattat ggggactagg agaaggcttg catgcgcgct gctcgcgctc
     2701 gtcgcggcgc tgtcgacggg ggtcgttccc gccggcgcgc acaacaaggg caagcattgc
     2761 aagaagggct acaagccgtg cctgcacccg gcgagcgact acgactgcgc gggcggcagc
     2821 ggcgacgggc ccaagtacgt ctacggcacc gtcaaggtga cgggctcgga cccctacggc
     2881 ctcgactccg acaacgacgg gatcggctgc gaggactaag ccctacggca ccctcgggat
     2941 ttgtcagaca tttgtctgac aaatccagag gccctcacca ggcatagtcc tcgggcgccg
     3001 tcttgtggcc cggccagatc tcgtcgagcc ggcccagctc gtcggccccc agcgtcagct
     3061 tcagcgctcg catcgagtcg tcgagctgct ccatcgtgcg cggcccgacg atcggtgcag
     3121 tgacggccgg ctggtgcagc agccacgcga gcgcgaccgt cgccgggtct tcgccgatct
     3181 ctccgcagaa gtcctcgtac gcctggatct ggtcgcggcg cgcctcgagc gcgtcgaggg
     3241 cgcgaccggt gtgccggcgc ttgccctcgc gctccttgcg caggacgccg ccgagcaggc
     3301 cgccgtgtag cgggctccag gggatcacgc cgacgccgaa gtcgagacac gcgggcagga
     3361 cctcgagctc gaccgcccgc gtcgcgaggt tgtagatcga ctgctcggag acgaggccgg
     3421 tgaagttgcg ccgttgcgcg gccccctgcg ccttggctat gtgccagccg gcgaagttgc
     3481 tcgacccgac gtagaggatc ttgccctgcg cacgcaggac ctccatggct tcccagatct
     3541 cgtcccacgg tgtgttccgg tcgacgtgat gcatctggta gaggtcgatg tagtcggtct
     3601 gcagacgcct gagcgatgcc tcgcacgcgt gccggatgtt gcgggccgac aggaacgtgt
     3661 cgttcggcca gtcgctcatc gagccgtaga gcttcgtcgc gatcacggtc ttctcgcgcc
     3721 gcccgccgcc ctgtgcgaac caacgcccga cgatctgctc ggtgatccct tcgcccttct
     3781 tccagccgta gacgttcgcg gtgtcgaaga agttgaagcc gagctcgagc gcgcgatcca
     3841 tgatggcgtg cgagtcgtct tccgacgtct gggggccgaa gttcatcgtg ccgaggcaga
     3901 gccgcgagac ctcgagcccg ctgcggccga gatgccggta gtccaagggg agcctcctag
     3961 caatggagct gaggagccct cattctccct cggccagatc gagcagcctc cgcagggtcg
     4021 ggagctgctc tcgatcgcgg ggacggttca gtgcctcctt cgaacggatc acgtcgcgca
     4081 gcgacgcgac gcgggcgcgg atgccaccga ggtcgacgtc gatcccgtcg cgagcgaggt
     4141 cgtcgtaacc gccggtcccg gcgggctcga acacgaggtc cagtcgtccg aacggcgtcg
     4201 agaggagcca cgtcacgtcg ccggcgagca tgtcttcgtc ccacgtccgt tcgacgatct
     4261 cgcccttgtg ggggtcgagc tcgcccgcgt cgagcgcctg gagagcgc
//
LOCUS       DASQDO010000351        17504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_894171, whole genome shotgun sequence.
ACCESSION   DASQDO010000351 DASQDO010000000
VERSION     DASQDO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894171"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VEV43_09815"
     CDS             complement(<1..501)
                     /locus_tag="VEV43_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23859.1"
                     /translation="MPKRVVVVGAIAVVVAIAAWFVLRPETGVPGDAPTENEMAEAVG
                     TEVMQHLYLGHVPGRSGEVLMVPKPHHFLISKWDMTQLAGPTLPLGSTHPNPWSYLTR
                     VPIVAHGAGVTPGVVEQPVDIAAIAPTYAEILGMTDFRAEADPQFLDAPTVSEIWDVS
                     NANRPIV"
     gene            complement(541..1242)
                     /locus_tag="VEV43_09820"
     CDS             complement(541..1242)
                     /locus_tag="VEV43_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HYP23860.1"
                     /translation="MRDRRRLARGLVLACWAAFFAWLHLSGEKTRYLGPRTYWVVVFG
                     AIVLGAAALAHLMTARNAPRRALEPRDALGIGALLVPLLAVAAVPSAELGALAASRKT
                     AGAGAASVGAIAPPVPTGDEAISFAEINYASESPEYAGEAGIAEGMEVELTGFVTHPD
                     GLPEGTLGLTRFHVSCCAADAVPYTVPLVADPGSRSDDVWLRVAGNLALRDGTYVLDP
                     REVTVVDAPSDPYLY"
     gene            complement(1239..2240)
                     /locus_tag="VEV43_09825"
     CDS             complement(1239..2240)
                     /locus_tag="VEV43_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HYP23861.1"
                     /translation="MAIGERAAPAARLPAQTPGWLLVPVAAVAAVLLVRLTALGGAAE
                     VETFVLVFTSIVVEALPFILLGALVSAAIEVYVPDRAFDRLARLPAAMQIPGAALGGL
                     AFPVCECGSVPVARRVIARGLHPGAGLAFMLAAPILNPIVIFSTVVAYGGRGLALEMA
                     AGRIVLGLVLAVVAGWALGNVTPEELLRPREGDASCDHAEPKRAAFVEHLAGDFFYMG
                     KFVIAGAGLAALLQTAIPQSLVSGVARTPVIGSLALMAIAFVLSLCSEADAFVAVSFT
                     PFPIGSQLAFLVFGPVVDAKLAFLYGATFRRRFVLRLLAVALPVTLAGSLWFEVMVG"
     gene            2264..3589
                     /locus_tag="VEV43_09830"
     CDS             2264..3589
                     /locus_tag="VEV43_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23862.1"
                     /translation="MLERHRKTPAEPSVTSLGAGRRTLVLLALVAVVGAPAAVLRGLC
                     AGHSCDEPANASSEVPFCSLPVEVRERVAAGFREGRSPDVLAVAAGEDLVAEGGVPWP
                     STSGRDETRVPLAFWGHGVNPAGEKIPPETGLDDVAPTIEAILGFDRPHPDVRSGSAI
                     EGVAGGGGPPRLVVEIVWIGRGSRDVGKGPVVLGFMDSGPATLHADTVSTPVDPAAVL
                     TTIGTGGRPSQHGITGEHVRDDAGVLRKAWSARSPVSVIATLGDDLDEELGQEPVIGM
                     VAARRSYLGAIGGNWYIDVDRDRFVKTEPGREPRDVRRLLDPAFGADEVPDLAVIAVE
                     GGRETLDEVFRVVLKRGAEISGQRLTVVFTATGDTGGTTVFEPPPGVAGLVERVVAGG
                     LFLDQDELARREVSEDEVLRSLVEARDASGDALFADAFPAIAVAFGRYC"
     gene            3589..4584
                     /locus_tag="VEV43_09835"
     CDS             3589..4584
                     /locus_tag="VEV43_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HYP23863.1"
                     /translation="MADVALRGPRDASWGNAAMVVVALGGAALLLQVLDVERIAEAQN
                     FLLVFSSLLIEAVPFVLLGAVVSAGIEVFVPGRFFERLTDLPRPLQLPAAALTGFAFP
                     VCECGSVPVARRLAAKGLSPAAAVTFMLAAPILNPVVLASTFVAYRGRDTLWTMMLGR
                     AGLGLVAAVAVGWVVANKSKGELLRPRPGDHDHHHEGETRPAAFFAHLSGDFLFMGRY
                     LVLGAVVAAALQTFVPQDVIGSVAGTPVVDLLAMMALAFVLSLCSESDAFVAASFVQF
                     GPGAQLAFLVFGPMVDTKLGFLYSATFTRGFFRTVVVSVAAVTLAGTLWIEVLVG"
     gene            4581..5321
                     /locus_tag="VEV43_09840"
     CDS             4581..5321
                     /locus_tag="VEV43_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HYP23864.1"
                     /translation="MTTAEATLERERARHWSAVKLASAAAMAAWAALFWWLLVSGRSF
                     LYLSDRTDWVVPMGAIILTVAVAGRLWSARGPHPEPLKRSDAWRLGAIVLPVVLTVAL
                     PPAALGSYATSRRSSFVSSGYTSSAADIESGELSLIDVAGAVRDEGAMKALVQRAGSE
                     VSFVGFVTRDSGMPADEFMLSRFLVSCCVADALSIQVRVVGAPPGKLAEDDWVRVTGA
                     MYPLGREVIVDASEVEPVDRPKRPYLNP"
     gene            complement(5318..6184)
                     /locus_tag="VEV43_09845"
     CDS             complement(5318..6184)
                     /locus_tag="VEV43_09845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYP23865.1"
                     /translation="MSMTIMEALDKRSPASVLALGLAAVGVIAAIDYATGAELAFSVF
                     YLAPIIAVAWVGPPAVAGTVAVAAGLAFPVSDLLAADETVAVWVPVWNFAVRSGTYLV
                     VAALTRALRRALDHERDLARIDPLTGALNARSFFELAEREVARSRRHGGPVTVVYLDL
                     DGFKEVNDEHGHSAGDEVLKEVASMLYTGMRPTDVVGRLGGDEFALLLPDTDAPAATN
                     PLNRMRAAVRTIADKRGYGVSVSVGAATFVRPPDDVNALLRAADGLMYEAKNAGKDRI
                     VQRTVEPAEARI"
     gene            6378..7415
                     /locus_tag="VEV43_09850"
     CDS             6378..7415
                     /locus_tag="VEV43_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HYP23866.1"
                     /translation="MGLRIFGALVAALLAAFAVMRYRKRQLRRGELLVVAIVAGALTV
                     AALAPSLLDPVLGALGFEPGGERRIIGLLVISNLFTLGLVFRSFSRDDLLSNELGDLV
                     DYMALKRLEESGFSPPIGACAVVVPAYNEADNLPAVLGQMPGEVAGLPIVPIVVADGC
                     TDATEATAVRYGAAVIRRDLRRGSGAAVRLGYQAALSAGARVVVTLDGDGQHDPKEME
                     LLVEPLLAGRADMVQGSRVLGSFEVESNVRSWGVKLFARLLTTLGRTRITDPSTGYRA
                     MTAEALRRLDLRQDQFYVSEVILDAARKGLAVVEVPITLRRRASGTTKKPTTFRYAWG
                     FSKAIVRTWLR"
     gene            7522..8664
                     /locus_tag="VEV43_09855"
     CDS             7522..8664
                     /locus_tag="VEV43_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYP23867.1"
                     /translation="MIFATPPVDAREAEVLQHVEDLRSRLRLWVAERRRWTGVLRRMS
                     FARQVQASNSIEGYNSTLDDAVAILEGEDPLDATEETTRALEGYRQAMTYVLQLADDP
                     HFAYSTDLVKSLHFMMMGYALDKSPGRYRLGHVYIRNDSTGEIVYEGPDAERVPSLVN
                     ELVDSLNEDSAAPAMVRAAMAHLNLVLVHPFRDGNGRMARGLQTLVLAREGILSAEFC
                     SIEEYLGRNTASYYDILARVGRGYWQPENDARPWVRYCLTAHYVQAQTLLRRIRESER
                     LWELLAAEADRHGLPERSVAALFEAARGFRVRNATYRPLSDVTEQTASRDLRQLVAVG
                     LLEPKGERRGRHYVASDRLRDLRARSLEVRIPVRDPFDESGAREAR"
     gene            complement(8671..9723)
                     /locus_tag="VEV43_09860"
     CDS             complement(8671..9723)
                     /locus_tag="VEV43_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYP23868.1"
                     /translation="MVMAADDERIALFLDFENLALGARDRGERLDMSVIMEALSERGR
                     VVVRRAYADWNLFSEHRVGLVGERIEMIEVPQRTGMVRKNAADIKLAVDALELAFQRE
                     FITTFVIGSGDSDFTPLVLKLRELNKKVIGVGVEGSTSELLPGACDEFLFYENLLGRG
                     SGSTPSRRRRRRKAEGVEPAGEADESISDIAELSRQVTRTLSGLQRTTDGSVLSSMIK
                     RALLRKDPTFAEGDYGFRTWGELMQHMASLGVVELRTGTAEGDPAVDFPAEGGGAERA
                     FRLLKETVEEIEQKNGSPPLSGLKDHLRKKKPGFSEKDYGYGGFLQFVKAARAKGVVD
                     MDWDDEAEDYFVSAGT"
     gene            complement(9729..10910)
                     /gene="rnd"
                     /locus_tag="VEV43_09865"
     CDS             complement(9729..10910)
                     /gene="rnd"
                     /locus_tag="VEV43_09865"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HYP23869.1"
                     /translation="MIFITKQDDLDDAVAAARTAGTVGLDTEFLREKTYRAKLCLVQM
                     AAEDDIYVIDVLEPLDLKGLADLIADPDVRVILHAGRQDLELFYEGWGVVPNNVYDVQ
                     LAAGFAGHGASLPYGRLVETLCGKRLVKGESYTDWCRRPLTEAQLSYAADDVRYLAQA
                     ARRLDAELQRLGRRDWVTQEMKAMESEEVYRTDPGEAWRKVGGRGSLSPKALGVLKEV
                     ARWREETAQRRDIPRGWVIKDPTMIEIARRAPSSVQALKGLRGFNSSEADRSGRDILA
                     AVDAGREGPSISLGAAPPKAAQARARMLSGLADAVVRARCEKAGMATELVATRSELEN
                     LLAEVVTGAVDEGRHRLMKGWRRELAGDAVVALAEGRVAVKAIPKSPFVEELDLEIEK
                     G"
     gene            complement(10907..12061)
                     /locus_tag="VEV43_09870"
     CDS             complement(10907..12061)
                     /locus_tag="VEV43_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23870.1"
                     /translation="MDFDLSPEQHEFQQVVRAFVEDAVRPNAERWDHEHELPMDAVKQ
                     MAELGLFGLPFPEEYGGSGADFLTLCLAIEELARVDSSLAITLEAAVGLGAMPIWHFG
                     TEEQKRKWLPPLATGESRAGFGLTEPGGGSDAGATRTTARLEGGEWVINGSKSFITNS
                     GTPITSFVTVTAVTGESQGHKEISAIVVPNDTPGFEVGKPYRKMGWWHSDTHELAYID
                     CRVPEENLLGQQGHGFRNFLKILDDGRIAIAALAVGLAQGCVDESVKYANERTAFGQA
                     IGSFQALQFKIADMATKTELARTAYHKAAWLKEQGRPYKKEAAMAKLYASTVAVDVAR
                     DAVQVHGGYGYIEEFPVCRHFRDSKILEIGEGTSEVMRILIARELGLPNA"
     gene            complement(12085..13356)
                     /locus_tag="VEV43_09875"
     CDS             complement(12085..13356)
                     /locus_tag="VEV43_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HYP23871.1"
                     /translation="MPAANPEKQEVISYERLYRLWEQNNWSATGIDFTEDAVHWQERL
                     TEKQREAALWNYAMFLVGEEAVARTLTPVLDAAPGFPESIFLTTQIVDEARHHVFFDR
                     FMREVAGIGSDTKSTLQGVERHLTWGFKQVFDELDRITDSLRKKPKDRELLTQTVALY
                     HLVVEGLLATPGQHFIQRYVDKLQILPGFREGIDNVSRDESRHVAFGVKLLGELVRSS
                     RDCRAAAIELFDRVLPWSVGVFIPPNLDREYTECFDFTLVEIYSFGLRGFETKLRRVG
                     IEPAELRVFSREDRSQSYEERSRRLWVLLEDGIMGDDRREPKVSAEAFEILFEGMARA
                     LDLEVARSLGGPIQWDFSDAEPWHIVVANGHAEAKPGTAGEPALRLETSAADFAKIAV
                     ERDDPRWSLLKRKLKVHGSLSAKAKLPKLFT"
     gene            13445..14635
                     /locus_tag="VEV43_09880"
     CDS             13445..14635
                     /locus_tag="VEV43_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP23872.1"
                     /translation="MDFTLTEEQQELQKWAHEFAEKEIRPVAAHYDETEEFPWPVLKK
                     AAEAGLYSVDIYLQAQQDPTGLTLPLLMEEVFWGCAGIGLAIFGTGLPLSALASVGTP
                     EQLFEWAPKMFGTPEDPKIAAFGVTEPQAGSDVRSLRTKAVRDGDEWVLNGTKVFITN
                     GQYADIYIIVATVDPELGHRGQASFIITKDDPGIRPGKKEKKLGIRASDTSEVILEDC
                     RIPVDRVLGGVDRLEGKLAKVRAGETSGRSSAALRTFEVTRPSVAIQAVGIARAALEF
                     ARDYAKDRVAFGVPIAQHQSIQNHLADMATAVEASRLLVHKAAWLAASGQPFTKAEGS
                     MSKLLAGETAVKVTETAVQILGGYGYIKDFPVEKWYRDSKIYTIFEGTSEIQRMVIAR
                     ALLA"
     gene            14770..15720
                     /locus_tag="VEV43_09885"
     CDS             14770..15720
                     /locus_tag="VEV43_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23873.1"
                     /translation="MLAHFEYGHLYKFLVSAGILLVVLGIALPWFLPTKNDLLLVPAA
                     DVVTLTPTAQRVLQREQERAEFLLQAWPWISLPSIGIGVALTLHGLKKWKERQRIADT
                     REEHERDLAAAQVQATASEVERKVDAEVALEEGPDLAPAHAADAPVTERTAAFRSKVV
                     EVEARLVSLLTEAVSDSFDVRPHVKAQDKNRISVLDAVLESHNARSEDIVVEVRYLRG
                     PTHVADYFLRTASLTELYTAATGRSAKPLLILVIGASRRPSDLAQHEGQVQRRLREAR
                     ALIARDVEAITIWEADLEEMTPADLRQKLGRRLLRLPQPT"
     gene            complement(15717..16574)
                     /locus_tag="VEV43_09890"
     CDS             complement(15717..16574)
                     /locus_tag="VEV43_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYP23874.1"
                     /translation="MTHHPNLGGNFTIGGDLPVHRLGFGAMQITGRGIWGEPSGVDGA
                     RALLRRVVESGVNLIDTADSYGPEVSERLIGETLHPYPEDVVIATKGGLLRPGPGSWQ
                     PHCTPSHLRSACEESLKRLKVERIDLYQLHTVDRKVPLEESIGALKELQDEGKIRHVG
                     VSNFSVSEIERARKIVEIVTVQNLYNLGDRSSEDVLSYCEREGIGFIPWYPLGSGDLL
                     SSSSLTEVAKMHESTPAQIALAWLLHKSPVMLPIPGTSSTEHFEENLGAGSIELSAEE
                     MSRIEKAGA"
     gene            complement(16597..>17504)
                     /locus_tag="VEV43_09895"
     CDS             complement(16597..>17504)
                     /locus_tag="VEV43_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYP23875.1"
                     /translation="IAERLAPSVTNLAVFGKVRGGRRMSGNGSGVVITSDGFVLTSAH
                     VVGRSDGGNASFSDGRETSFEIVGCDPLSDLAVVRARGDDFEPAELGDAARLKVGQLV
                     VAIGNPLGFGGTVTAGVVGALGRSFVTQANGTARFVENVIQTDAALHPGNSGGALVES
                     RGTVVGINTALVGPGMGQGLGLAVPVNETTLKIVSALMKEGRVRRAWFGVAGGTRPLP
                     PRLAREVGRERGIEVVQVVAGSPAEQAGLRPEDIILEVHGTPVAGMDDLQRLMDGTLA
                     GTTVSLLVYRDRLLELSITPAELAA"
BASE COUNT         2629 a         6114 c         6051 g         2710 t
ORIGIN      
        1 gactatcggg cggttcgcgt tcgacacgtc ccagatctcc gagaccgtcg gcgcgtcgag
       61 gaactgcggg tcggcctcgg cgcggaagtc cgtcatcccg aggatctcgg cgtacgtcgg
      121 ggcgatcgca gcgatgtcca ccggctgctc gacgactccc ggggtcacgc ccgcaccgtg
      181 ggcgacgatc gggacgcggg tgaggtagct ccacgggttg gggtgcgtgc tgcccagcgg
      241 cagcgtcggc ccggcgagct gcgtcatgtc ccacttgctg atcaggaagt ggtggggctt
      301 ggggaccatg aggacctcgc ccgaccggcc cgggacgtga ccgaggtaga ggtgctgcat
      361 gacctcggtc ccgacggcct cggccatctc gttctcggtc ggcgcgtcgc ccggaacgcc
      421 ggtctcgggg cgcagcacga accacgccgc gatcgcgacg acgacggcga tcgcgcccac
      481 tacgacgacg cgcttcggca agacatctcc ttgctggggg cccggctgga cgggcgcaga
      541 ctagtacagg tacggatccg agggagcgtc gacgaccgtc acttcccgcg ggtcgaggac
      601 gtaggtgccg tccctcagcg ccaggttacc ggcgacccgc agccatacgt cgtcggacct
      661 gctcccgggg tcggcgacga gcgggaccgt gtagggcacg gcgtcggcgg cacagcacga
      721 cacgtggaag cgcgtgagcc ccagcgtccc ttcggggagc ccgtccgggt gcgtgacgaa
      781 gccggtcagc tcgacctcca tcccctcagc gatgccggcc tcgcccgcgt actcgggaga
      841 ctcgctcgcg tagttgatct cggcgaacga gatcgcctcg tcgccggtcg ggaccggcgg
      901 ggcgatggca cccaccgacg cggcccccgc acctgcggtc ttgcgggacg cggcgagcgc
      961 gccgagctct gccgagggga ccgcggcgac cgccagcaag gggacgagca gggcgccgat
     1021 cccgagcgcg tcacgaggct cgagagcgcg cctcggcgcg ttgcgcgccg tcatcaggtg
     1081 cgcgagcgcg gccgcgccga ggacgatcgc gccgaagacc acgacccagt acgtgcgggg
     1141 gccgaggtag cgcgtcttct cgccgctcag gtgcagccac gcgaagaacg cggcccagca
     1201 cgcgaggacg aggccgcggg cgagcctccg gcggtccctc acccgaccat cacctcgaac
     1261 cacagcgacc cggcgagcgt caccggcagc gcgacggcga gcagccgcag cacgaaccgt
     1321 cgccggaacg tcgccccgta caagaacgcc agcttcgcgt cgacgaccgg gccgaacacg
     1381 aggaacgcga gctgcgagcc gatggggaac ggcgtgaacg acaccgcgac gaacgcgtcc
     1441 gcctccgagc acagcgacag cacgaacgcg atcgccatca acgcgagcga tccgatgacc
     1501 ggcgtgcgcg cgacgcccga gacgaggctc tgcgggatgg cggtctggag cagcgccgcc
     1561 agcccggccc ccgcgatcac gaacttgccc atgtagaaga agtcaccggc gaggtgctcg
     1621 acgaacgcgg cccgcttcgg ctcggcgtgg tcgcaggacg catcgccctc tcgtggccgc
     1681 agcagctcct ccggcgtgac gttgccgagg gcccacccgg ccacgacggc gagcacgagc
     1741 ccgaggacga tccgccccgc cgccatctcg agcgcgagcc cccggccccc gtaggcgacg
     1801 acggtcgaga agatgacgat cgggttcagg atcggcgccg cgagcatgaa cgcgagcccc
     1861 gctccgggat ggaggccgcg ggcgatcacg cggcgagcga ccgggacgct cccgcactcg
     1921 cacaccggga aggcgaggcc tccgagcgcg gcgccgggga tctgcatcgc cgcggggagc
     1981 cgggcgaggc ggtcgaaggc tcggtcgggg acgtagacct cgatggccgc ggacacgagc
     2041 gctccgagca ggatgaacgg gagcgcctcg acgacgatcg aggtgaacac gaggacgaac
     2101 gtctcgacct ccgcggcccc tccgagcgcc gtgagcctca cgagcaggac ggcggcgacg
     2161 gccgcgaccg gcaccagcaa ccaccccggc gtctgcgccg gaagccgagc ggcgggggcc
     2221 gcgcgctctc cgatagccat gggcttcacg gtagcttgag gccgtgctgg aacggcaccg
     2281 gaagacgccc gcggagccct cggtgacgag cctcggggcc gggcgccgga ccctcgtcct
     2341 cctcgcgctc gtcgccgtcg tgggtgcgcc cgcggcggtg ctgcgcggtc tctgcgcggg
     2401 gcattcctgc gacgagcccg cgaacgcctc ctccgaggtc cccttctgct cgctcccggt
     2461 cgaggtccgg gagcgggtcg ccgcaggctt ccgggagggc cggtcccccg acgtcctggc
     2521 ggtcgccgcc ggtgaggacc tcgtcgccga aggtggtgtc ccctggccgt cgacctccgg
     2581 ccgtgacgag acgcgggtcc cgctcgcctt ctggggccac ggggtgaacc ccgccggcga
     2641 gaagatcccg ccggaaaccg ggctcgacga cgttgccccc acgatcgagg ccatcctcgg
     2701 gttcgaccgt ccgcatccgg acgtgcgatc cggatccgcg atcgaaggcg tcgcgggggg
     2761 cggcgggcct ccccgcctcg tcgtcgagat cgtctggatc gggcgcggct cccgcgacgt
     2821 cggcaagggg ccggtcgtgc tcggcttcat ggactccggc ccggcgacgc tccatgccga
     2881 caccgtctcg acgcccgtcg accccgcagc cgtcctgacg acgatcggga ccgggggccg
     2941 gccctcgcag cacgggatca ccggggagca cgtgcgcgac gacgccggcg tcttacgcaa
     3001 ggcgtggtcg gctcgcagcc cggtatcggt catcgcgacg ctgggtgacg acttggacga
     3061 ggagctgggg caggaacccg tcataggcat ggtcgcggcc cgccgctcgt acctgggggc
     3121 gatcggcggc aactggtaca tcgacgtcga ccgggaccgc ttcgtgaaga ccgagccggg
     3181 aagggagccg cgcgacgtgc ggaggctgct cgatccggcg ttcggagcgg acgaggtgcc
     3241 ggacctcgcc gtgatcgcgg tcgagggcgg gcgcgagacg ctcgacgagg tcttccgcgt
     3301 cgtgctgaag cgcggcgcgg agatatctgg gcagcggctg accgtggtct tcacggcgac
     3361 cggcgacacc ggcgggacca cggtcttcga gcccccaccc ggcgtcgccg gtctcgtcga
     3421 acgggtcgtc gccggcgggc tgttcctcga ccaggacgag ctcgcgcgcc gcgaggtcag
     3481 cgaggacgag gtcctccgca gcctggtcga agcgcgggac gcgtccggtg acgcgctgtt
     3541 cgcggacgcg ttcccggcga tcgcggtcgc gttcgggagg tactgctgat ggccgacgtc
     3601 gcgctgcggg ggccgagaga cgcgtcgtgg gggaacgccg cgatggtcgt cgtcgccctg
     3661 ggcggcgccg cgctcctgct acaggtcctg gacgtcgagc gcatcgcgga ggcgcagaac
     3721 ttcctgctgg tgttctcgtc gctgttgatc gaggcggtgc cgttcgtgct gctcggcgcg
     3781 gtcgtctcgg ctgggatcga ggtcttcgtg ccgggccgct tcttcgaaag gctgaccgac
     3841 ctcccccggc ccctgcagct tccggccgcg gccctgaccg gcttcgcctt cccggtctgc
     3901 gagtgcggga gcgtcccggt ggcgcgccgc cttgcggcga aggggttgtc gcccgcggcc
     3961 gcggtcacgt tcatgctcgc cgcgccgatc ctgaaccccg tcgtgctcgc gtccacgttc
     4021 gtcgcctacc gcgggcgcga caccttgtgg acgatgatgc tcgggcgcgc cgggctcggc
     4081 ctcgtcgccg ccgtcgcggt cggctgggtc gtggcgaaca agtccaaggg cgagctcctg
     4141 cgcccccgtc ccggcgacca cgaccatcac cacgagggcg agacgcgtcc ggccgcgttc
     4201 ttcgcacacc tctccggcga cttcctgttc atgggccggt acctcgtgct cggcgccgtc
     4261 gtcgccgcgg ccctccagac gttcgtgccc caggacgtga tcggttccgt cgcggggacc
     4321 ccggtcgtcg acctcctggc gatgatggcg ctcgcgttcg ttctgtcgct gtgctcggag
     4381 tccgacgcgt tcgtcgcggc gtcgttcgtc cagttcgggc cgggggccca gctcgcgttc
     4441 ctcgtgttcg ggccgatggt cgacacgaag ctcgggttcc tgtactcggc gacgttcacg
     4501 cgcggcttct tccggacggt cgtcgtgtcg gtcgccgcgg tcacgctcgc cgggacgctg
     4561 tggatcgagg tgctagtcgg atgacgacgg ccgaggcgac gctcgagcgc gagagagcgc
     4621 ggcactggtc cgccgtgaag ctggcgtcgg ccgcggcgat ggcggcgtgg gctgcgttgt
     4681 tctggtggct gctcgtctcg ggtcggtcgt tcctgtacct gtcggaccgg accgactggg
     4741 tcgtgcccat gggggcgatc atcctgacgg tcgcggtcgc cggccggctg tggtcggcga
     4801 gggggccgca tcccgagccg ctgaagcgtt ccgacgcgtg gcgcctcggc gcgatcgttc
     4861 tccccgtcgt cctgacggtc gcgctgccgc cggcggccct cggctcctac gcgacgtcgc
     4921 ggcgttcgag cttcgtcagc tccggctata cgtcgtccgc agccgacatc gagtcgggcg
     4981 agctctcgct gatcgacgtc gccggggcgg tgcgcgacga gggcgcgatg aaggcactgg
     5041 tgcagcgcgc cggctccgag gtctcgttcg tgggcttcgt cacgcgcgac tccggcatgc
     5101 cggcggacga gttcatgctc tcgcgcttcc tggtctcgtg ctgcgtcgcc gacgcattgt
     5161 cgatccaggt gcgcgttgtc ggcgcgccgc cgggaaagct ggcggaagac gactgggtcc
     5221 gggtgaccgg tgcgatgtac ccgctgggcc gcgaggtgat cgtcgacgcg tcggaggtcg
     5281 agcccgtcga ccgcccgaag cggccctacc tgaatcccta gatacgcgcc tcggcgggct
     5341 cgacggtccg ctgcactatc cggtccttgc ccgcgttctt cgcttcgtac atgagcccgt
     5401 cagccgcgcg cagcagcgcg ttcacgtcgt ccgggggccg gacgaacgtc gcggccccca
     5461 cgctgaccga gaccccgtag cccctcttgt cggcgatcgt ccgcaccgcc gcgcgcatcc
     5521 ggttgagggg attcgtcgcg gccggcgcat cggtgtcggg gagcaggagc gcgaactcgt
     5581 cgccgccgag gcggcccacg acgtcggtcg ggcgcatgcc cgtgtacagc atcgacgcga
     5641 cctccttgag gacctcgtcg ccggcggagt ggccgtgctc gtcgttgacc tccttgaagc
     5701 cgtcgaggtc gaggtacacg accgtgaccg ggcccccgtg ccgccgcgac cgcgcgacct
     5761 cgcgctcggc cagctcgaag aacgagcgcg cgttgagcgc gcccgtcagg gggtcgatcc
     5821 gcgccaggtc ccgttcgtgg tcgagcgcac ggcgcagcgc gcgcgtgagc gccgcgacga
     5881 cgagatacgt gcccgaccgc actgcgaagt tccagaccgg gacccacacg gccacggtct
     5941 cgtctgcagc gagcaggtcc gaaaccggga acgcgagccc ggcggcgacc gcgaccgtcc
     6001 ccgcaaccgc gggcggcccg acccaagcga ccgcgatgat cggcgccaga tagaagaccg
     6061 agaacgccag ctcggccccg gtcgcgtagt cgatcgccgc gatcacgccc actgcggcca
     6121 gccccagcgc aaggacggat gccggcgagc gcttgtccag cgcctccatg atcgtcatgg
     6181 acatcccgag agctgccctc ctgctacgac ggaactttgc ccgaagggta aaggaggagg
     6241 tcgtattgat cccggcaccg cgcgacgctc cggtgcggac gtggcccgtt ctgctagcct
     6301 gctcgtggcc gcatccggcc tcacccgaac catctggacg tcttggcagg ctcgaagccg
     6361 ccgaagggac ctgacgcatg ggcctgagga tctttggtgc tctcgttgcc gcgctgctcg
     6421 cggccttcgc cgtcatgcgc taccgcaagc ggcagctgcg gcgcggcgag ctcctcgtcg
     6481 tcgcgatcgt cgccggcgcg ctcacggttg cggccctcgc gccgtcgctg ctcgaccccg
     6541 tcctcggtgc gctgggattc gagcccggcg gcgagcgcag gatcatcggc ctgctcgtca
     6601 tctcgaacct gttcacgctc gggctggtct tccgcagctt ctcgcgcgac gacctgctgt
     6661 cgaacgagct cggcgacctc gtcgactaca tggcgttgaa gcgcctcgag gagagcggct
     6721 tctcgccgcc gatcggtgcg tgcgccgtcg tcgtcccggc ttacaacgaa gcggacaacc
     6781 tccccgcggt cctgggtcag atgcccggcg aggtcgcggg gctcccgatc gtcccgatcg
     6841 tggtcgcaga cggctgcacg gacgccaccg aggcgacggc ggtcaggtac ggcgcggccg
     6901 tgatccgccg cgatctcagg cgcggctcgg gggccgcggt caggctcggc taccaggccg
     6961 cgctgagcgc cggggcaagg gtcgtcgtca ccctcgacgg tgacggccag cacgacccga
     7021 aggagatgga gctcctcgtc gagccgctgc tcgccgggcg ggccgacatg gtccagggct
     7081 cgagggtcct ggggtcgttc gaggtcgagt ccaacgtgcg gagctggggc gtcaagctgt
     7141 tcgcccggct cctgacgacg ctcggccgga cccggatcac ggacccgtcg acgggctacc
     7201 gggcgatgac ggccgaggcc ctgcgccggc tggacctgcg ccaggaccag ttctacgtgt
     7261 ccgaggtgat cctggacgcc gcccgcaagg gcctggcggt ggtagaggtg ccgatcacgc
     7321 tacgccgccg cgcgagcggg acgacgaaga agccgacgac gttccgctac gcctggggct
     7381 tcagcaaggc gatagtgagg acctggctcc gctagccggg ccgcaagcgt aagcccctgg
     7441 gcttgacaga ttgacggttg tcagccatct tcatgactga taatatacgt cataaagacg
     7501 gaaccaatcg gagttacagt catgatcttc gcgaccccac ccgtagatgc ccgcgaggcg
     7561 gaggtcctcc agcacgtgga ggacctcagg tcgcgcctac gcctctgggt cgcagagcgg
     7621 cgccgctgga cgggggtcct tcgccggatg tccttcgccc ggcaggtcca ggcgtcgaac
     7681 agcatcgagg gctacaactc gacgctcgac gacgccgtcg ccatcctcga aggcgaagat
     7741 cccctggacg ccaccgaaga gacgacgcgc gcgctcgagg gctaccggca agcgatgacc
     7801 tacgttctcc agctcgccga cgatccccac ttcgcgtact cgacggactt ggtaaagagc
     7861 ctccacttca tgatgatggg gtacgcgctc gacaagagtc ccggccgcta ccggctcggg
     7921 cacgtctaca tccgtaacga ctcgacgggc gagatcgtct acgagggacc cgacgccgaa
     7981 cgtgttcctt ctctggtgaa cgagctcgtc gactcgctga acgaagactc cgcggccccc
     8041 gcgatggtcc gcgcggcgat ggcgcacctg aacctcgtgc tggtgcaccc gttccgcgac
     8101 gggaacggac gcatggcgcg cgggctccag acactggtcc tcgcgagaga ggggatcctt
     8161 tcggcggagt tctgcagcat cgaggagtat ctggggcgga acactgcgag ctattacgac
     8221 atcctggcgc gggtcgggcg cgggtactgg cagccggaga acgacgcgcg cccgtgggtc
     8281 cgctactgcc tcacggcgca ctacgtccag gcgcagacgc tgcttcgcag gatccgtgaa
     8341 tccgaacggc tgtgggagct gctcgcggcc gaggcggacc ggcacgggtt gccggaacgc
     8401 tcggtcgcgg ccctcttcga ggccgcacga gggttccgcg tccgcaacgc gacctatcgt
     8461 ccgctcagcg acgtcacgga gcagaccgcg agccgcgacc tacggcagct ggtcgccgtc
     8521 ggactgctgg aaccgaaggg cgaacggcgc gggcggcatt acgtcgcgtc cgaccggctg
     8581 cgcgacctcc gcgctcggtc gctggaggtg aggatcccgg tccgcgaccc gttcgacgag
     8641 agcggcgcgc gcgaggcgcg ataacccggg ctaggtgccg gccgagacga agtagtcctc
     8701 ggcctcgtcg tcccagtcca tgtccaccac acccttcgcc cgggcggcct tcacgaactg
     8761 caggaagccg ccgtagccgt agtccttctc gctgaagccc ggcttcttct tgcgcaggtg
     8821 gtccttcagg ccggacaggg gcggcgaccc gttcttctgc tcgatctcct cgaccgtctc
     8881 cttgaggagc cggaacgccc gctcggcccc cccgccctcg gccgggaagt ccaccgccgg
     8941 gtcgccctcg gccgtgccgg tccgcagctc gacgacgccg agcgacgcca tgtgctgcat
     9001 cagctcgccc cacgtccgga agccgtagtc gccctcggcg aacgtcgggt ccttgcgcag
     9061 cagcgcgcgc ttgatcatcg aagagagcac cgagccgtcc gtcgtccgct ggaggccact
     9121 caacgtccgc gtgacctgac gggagagctc cgcgatgtcc gagatcgact cgtcggcctc
     9181 gcccgcgggc tcgaccccct cggccttgcg ccgccggcgc cgccgcgacg gcgtgctccc
     9241 cgagccgcgg ccgagcaggt tctcgtagaa gaggaactcg tcgcacgcgc cgggcaggag
     9301 ctcggaggtg gatccctcga cgccgacgcc gatcaccttc ttgttgagct cgcggagctt
     9361 cagcacgagc ggcgtgaagt cgctgtcgcc ggacccgatc acgaacgtcg tgatgaactc
     9421 tcgctggaac gcgagctcga gcgcgtcgac ggcgagcttg atgtcggccg cgttcttgcg
     9481 gaccatgccg gtgcgctgcg gcacctcgat catctcgatg cgctcgccga cgagcccgac
     9541 ccggtgttcc gagaacaggt tccagtcggc gtacgcgcgc cgcacgacga cgcggccgcg
     9601 ttccgacaac gcctccatga tcacggacat gtcgagtcgc tcgccgcggt cgcgcgcgcc
     9661 gagcgcgagg ttctcgaagt ccaggaagag agcgatcctc tcgtcgtccg ctgccatcac
     9721 catgtgatct accccttttc gatttcgagg tcgagctctt cgacgaacgg ggacttcggg
     9781 atcgccttca cggcgacgcg gccctcggcc agcgctacga cggcgtcacc cgcgagctcg
     9841 cgccgccaac ccttcatgag ccggtgccgg ccctcgtcga ccgcgccggt gacgacctcg
     9901 gcgagcaggt tctcgagctc cgagcgagta gccaccagct cggtcgccat gcccgccttc
     9961 tcgcagcgcg cccgcacgac cgcgtcggcc agccccgaca gcatccgcgc acgcgcttgt
    10021 gcagccttcg gcggcgcggc ccccagagag atcgacggtc cttcgcgccc cgcgtcgacc
    10081 gccgcgagga tgtcgcgccc cgagcgatcc gcctccgacg agttgaaccc ccggagcccc
    10141 ttgagggcct ggacggagga gggggcgcgg cgcgctatct cgatcatcgt cgggtccttg
    10201 atgacccagc cgcgcgggat gtcgcggcgc tgcgcggtct cctctcgcca gcgggcgacc
    10261 tccttgagca cgccgagggc cttcggggag agggagccgc ggcccccgac cttgcgccac
    10321 gcctcgcccg gatcggtccg gtagacctcc tcggactcca tcgccttcat ctcctgagtg
    10381 acccagtcgc ggcggccgag acgctggagc tccgcgtcca gtcgccgcgc ggcctgagcc
    10441 aggtagcgca cgtcgtcggc cgcatagctc agctgtgcct cggtcaacgg gcgccggcac
    10501 cagtcggtgt acgactcgcc cttgacgagc cgcttgccgc acagggtctc gacgagccgg
    10561 ccgtacggga gcgacgcgcc gtggccggcg aaccccgcgg cgagctggac gtcgtagacg
    10621 ttgttcggga ccacgcccca gccctcgtag aacagctcga ggtcctgtcg tcccgcgtgc
    10681 aggatcacgc gcacgtcggg gtcggcgatg aggtcggcga gccccttgag gtcgagcggc
    10741 tcgagcacgt cgatcacgta gatgtcgtcc tcggccgcca tctgcacgag gcacagcttg
    10801 gcgcggtagg tcttctcgcg gaggaactca gtatcgagcc cgacggtccc ggcggtgcgc
    10861 gccgcggcta cggcgtcgtc gaggtcgtcc tgcttcgtga tgaagatcaa gcgttcggta
    10921 gccccagctc gcgcgcgatc aggatccgca tgacctcgga cgtcccctct ccgatctcga
    10981 ggatcttcga atcgcggaag tgcctgcaca cggggaactc ctcgatgtag ccgtagccac
    11041 cgtggacctg gacggcgtcg cgcgcgacgt cgacggcgac cgtcgacgcg tacagtttcg
    11101 ccatcgcggc ctccttcttg taaggacgcc cctgctcctt cagccacgcg gccttgtggt
    11161 acgcggtgcg ggcgagctcg gtcttcgtcg ccatgtccgc gatcttgaat tgcagcgcct
    11221 ggaagctccc gatcgcctgg ccgaacgccg tgcgctcgtt cgcgtacttg acgctctcgt
    11281 ccacgcaccc ttgcgcaaga ccgacggcga gggccgcgat cgcgatccgt ccgtcgtcga
    11341 ggatcttgag gaagttacgg aagccgtggc cctgctggcc gagcaggttc tcctcgggca
    11401 cgcggcagtc gatgtacgcg agctcgtgcg tgtccgagtg ccaccacccc atcttccggt
    11461 acggcttgcc gacctcgaag ccgggcgtgt cgttcgggac gacgatcgcc gagatctcct
    11521 tgtgcccctg ggactccccg gtcaccgcgg taacggtgac gaacgacgtg atcggtgtgc
    11581 ccgagttcgt gatgaacgac ttcgagccgt tgatgaccca ctcgccgcct tcgagccggg
    11641 cggtcgtgcg cgtggcgccg gcgtcggaac cgccccccgg ctcggtcagc ccgaaccccg
    11701 cacgggactc acccgtcgcg agcggcggga gccacttgcg cttctgctcc tcggtcccga
    11761 agtgccagat cggcatcgcg ccgagcccga cggccgcctc cagcgtgatc gccagagacg
    11821 agtcgacacg agccagctcc tcgatcgcga ggcagagcgt caggaagtcg gcgccggagc
    11881 ccccgtactc ctcggggaac gggagcccga agaggccgag ctcggccatc tgcttgacgg
    11941 cgtccatcgg gagctcgtgc tcgtggtccc agcgctcggc gttgggccgc acggcgtcct
    12001 cgacgaaggc ccggacgacc tgctggaact cgtgttgttc gggagagaga tcgaagtcca
    12061 tcccggcaga ctatccgtct gcgtctacgt gaacagctta ggcagcttcg ccttcgcgga
    12121 cagcgatccg tggaccttca gcttgcgctt cagcagcgac cagcgcgggt cgtcgcgctc
    12181 gaccgcgatc ttggcgaagt cggccgccga ggtctcgagc cggagagccg gctcacccgc
    12241 cgtcccgggc ttggcctccg cgtgaccgtt cgcgacgacg atgtgccagg gctccgcgtc
    12301 gctgaagtcc cactggatcg ggccgccgag cgagcgcgcg acctcgaggt cgagagcgcg
    12361 ggccatccct tcgaacagga tctcgaacgc ctcggcgctc accttcggct cgcgcctgtc
    12421 gtcgcccatg atcccgtcct cgagcaggac ccacaggcgc cgcgaccgct cctcgtagga
    12481 ctggctgcgg tcctcgcgcg agaacacgcg caactcggcc ggctcgatcc cgacgcgccg
    12541 gagcttcgtc tcgaagccgc gcaacccgaa cgagtagatc tcgacgagcg tgaagtcgaa
    12601 gcactcggtg tactcgcggt cgaggttcgg cgggatgaag acgccgacgg accacgggag
    12661 cacgcggtcg aacagctcga tcgcggcggc gcggcagtcg cgggaggagc ggacgagctc
    12721 gccgagcagc ttcacgccga acgcgacgtg acgcgattcg tcgcgcgaca cgttgtcgat
    12781 cccctcgcgg aagccgggca ggatctggag cttgtcgacg tagcgctgga tgaagtgctg
    12841 tcccggcgtc gcgaggaggc cctcgacgac gaggtggtac agcgcgacgg tctgcgtcag
    12901 cagctcgcgg tccttcggct tcttgcgcag cgagtcggtg attcggtcga gctcgtcgaa
    12961 gacctgcttg aagccccatg tgaggtgtcg ttcgactccc tgcagggtcg acttcgtgtc
    13021 gctcccgatg cctgccacct cgcgcatgaa gcggtcgaag aagacgtggt gccgggcctc
    13081 gtcgacgatc tgggtggtga ggaagatgct ctcggggaag ccgggggccg cgtcgagcac
    13141 cggcgtcagc gtccgggcta cggcctcctc gccgacgagg aacatcgcgt agttccacag
    13201 ggcggcctcg cgctgcttct cggtgaggcg ttcctgccag tggacggcgt cctcggtgaa
    13261 gtcgatcccc gtcgccgacc agttgttctg ctcccagagg cggtacagcc gctcgtagga
    13321 gatgacttcc tgcttctcgg ggttggctgc cggcacggga cctccttatt gctccgccgt
    13381 gggctatcct gacaccatct ctaacttgac tggagagtca agttcaccac acccggagga
    13441 tgggatggac ttcacgctga ccgaagagca gcaggagctg cagaagtggg cccacgagtt
    13501 cgccgagaag gagatccggc ccgtcgccgc ccactacgac gagaccgagg agttcccctg
    13561 gccggtcctc aagaaggcgg ccgaggcggg gctctactcc gtcgacatct atctgcaggc
    13621 ccagcaggac ccgacggggc tgacgcttcc gttgctgatg gaggaagtgt tctgggggtg
    13681 tgccgggatc gggctcgcga tcttcgggac ggggctgccg ctgtcggctc tggcttcggt
    13741 cggcacaccc gagcagctgt tcgagtgggc gccgaagatg ttcgggacgc ccgaagaccc
    13801 gaagatcgcc gcgttcggcg tgacggagcc gcaggcgggg tcggacgtgc ggtcgctgcg
    13861 gaccaaggcc gtgcgtgacg gcgacgagtg ggtcctgaac ggcacgaagg tcttcatcac
    13921 gaacggccag tacgcggaca tctacatcat cgtggcgacg gtcgaccccg agctcggcca
    13981 ccgcggccag gcctcgttca tcatcacgaa ggacgacccc gggatccggc ccgggaagaa
    14041 ggagaagaag ctcggcatcc gcgcgtcgga cacgtccgag gtgatcctcg aggactgccg
    14101 catcccggtg gaccgggtcc tcggtggggt cgaccgtctc gaagggaagc tggcgaaggt
    14161 ccgagccggc gagacgagcg gccgttcctc ggcggcgctc cggacgttcg aggtgacgcg
    14221 tccgtcggtt gcgatccaag cggtcggcat tgcgcgcgcg gccctcgagt tcgcgaggga
    14281 ctacgcgaag gaccgggtcg cgttcggcgt gccgatcgcg cagcaccagt cgatccagaa
    14341 ccatttggcc gacatggcga cggccgtcga ggcgtcgagg ctgctcgtgc acaaggcggc
    14401 atggctggcg gcgagcggtc agccgttcac gaaggccgag ggctcgatga gcaagctgct
    14461 ggcgggcgag acggccgtga aggtgacgga gacggccgtg cagatcctcg ggggctacgg
    14521 ctacataaag gacttcccgg tagagaaatg gtaccgggac tcgaagatct acacgatctt
    14581 cgagggcacg tccgagatcc agcggatggt gatagcccgg gctttgctgg cctagcgttc
    14641 ccggccgtcc attgcgcaag gagatgcgct ttccgtacgt ttttgcgcaa tgaagcgtgc
    14701 cccggcgggg tgagctttct agaagagggg ctcttgcgcg gggtgggacc cgacgcttag
    14761 cttcgacgga tgctcgcgca cttcgagtac ggccatctct acaagttcct ggtctcggcg
    14821 gggatcttgc tcgtggtgct cggcatcgca ctcccttggt tccttccaac caagaacgat
    14881 ctcctgttag tccccgcggc ggacgtggtg acgttgacac caactgctca gcgcgttctg
    14941 caacgggaac aggaacgcgc cgagttcctc cttcaggcct ggccctggat aagccttccg
    15001 tcgatcggca tcggggttgc gttgaccctg cacgggttga agaagtggaa ggagcgtcag
    15061 aggatcgccg acacgagaga ggagcacgaa cgggacttgg cggctgcgca ggtccaggcc
    15121 accgccagcg aggtagagcg caaagtagac gccgaagtag ctctcgagga gggcccggac
    15181 ctggcccctg cccacgctgc ggacgcaccc gtgacggaaa ggacagccgc attcagaagc
    15241 aaggtcgtcg aagtagaagc tcgtctggtc tcgctactga ccgaagcggt gagtgactcg
    15301 ttcgacgttc gtcctcacgt caaggcccag gacaagaatc gaatctctgt actggacgcc
    15361 gtcctcgaat cacacaacgc gcgcagcgag gacatcgtcg tcgaagtgag gtatctgcgc
    15421 ggcccgactc acgttgcgga ctacttcttg cggacggcct cgctgaccga gctgtacaca
    15481 gccgcgacgg gccgctccgc caaaccgctg ctgatcctgg tcatcggagc atcacggcga
    15541 ccgtcagact tggcccagca tgagggtcag gttcagagac gtctccgcga agccagggcg
    15601 ctcatagccc gggatgtcga ggcgatcacg atttgggagg ccgacctcga ggagatgaca
    15661 cctgcggacc ttcggcagaa gctgggccgc cgcctcctac gcctacctca gccgacctag
    15721 gcccccgctt tctcgattcg gctcatttct tcggcgctca gctctatcga accggccccc
    15781 aggttctctt cgaagtgctc ggtcgaggac gtccccggga tcggaagcat caccggcgac
    15841 ttgtggagta gccacgccag cgctatctgg gccggggtcg attcgtgcat cttcgccacc
    15901 tcggtcagcg aagaggacga caagagatcg ccagagccaa gcgggtacca cgggatgaaa
    15961 ccgatcccct cgcgctcgca gtacgaaagc acgtcctccg acgaacggtc gcccaggttg
    16021 tagaggttct gcaccgtcac gatctccacg atcttccggg ccctctcgat ctccgagacg
    16081 ctgaagttcg acaccccgac gtggcggatc ttgccctcgt cctgcagctc cttcagcgca
    16141 ccgatcgact cctccagcgg caccttccgg tcgaccgtgt gcagctggta caggtcgatc
    16201 cgctcgacct tcaggcgttt caggctctct tcacacgccg aacgaagatg cgacggcgtg
    16261 cagtgcggct gccagctccc cggccccggt cgcagcagcc cgcccttcgt cgcgatgacg
    16321 acgtcttccg gatacggatg gagcgtctcg ccgatcagtc gctcgctcac ctcgggcccg
    16381 tacgagtcgg ccgtgtcgat caggttcacg cccgactcga cgacccgccg cagcagcgca
    16441 cgcgcgccgt cgacgccact cggctcgccc cagatcccgc ggccggtgat ctgcatcgcg
    16501 ccgaagccga gtcggtgcac cggaaggtcg ccgccgatcg tgaagttgcc gccgaggttc
    16561 gggtggtgag tcatcgaagc tccctccggg aagaagtcac gctgccagct ccgcgggcgt
    16621 gatcgacagc tcgagcagcc ggtctcggta tacgaggagc gagaccgtcg tgccggcgag
    16681 cgtgccgtcc atcagccgct gcaggtcgtc catgcccgcg acgggcgttc cgtgaacctc
    16741 caggatgatg tcctcggggc gcagcccggc ctgctcggcg ggacttcccg ccacgacctg
    16801 gacgacctcg atcccgcgct cgcgtccgac ctcgcgagcg agccgcggcg gcagcggccg
    16861 ggtccctccc gcaacgccga accaagcccg ccggacgcgc ccttccttca tcagggcgga
    16921 gacgatcttg agcgtcgtct cgttcaccgg gaccgcaagc ccgaggccct gccccatgcc
    16981 cggcccgacc agcgcggtgt tgatcccgac gacggtgccc cgcgactcga ccagcgcgcc
    17041 tccggagttc cccggatgaa gcgccgcgtc ggtctggatc acgttctcga cgaagcgcgc
    17101 agtgccgttc gcctgcgtga cgaacgaccg tccgagcgcg ccgacgacac cggcggtcac
    17161 ggtcccgccg aagccgagcg ggttgccgat cgcaaccacg agctggccga ccttcaagcg
    17221 agcggcgtct ccgagctccg ccggctcgaa gtcgtcaccg cgagcgcgca cgaccgcgag
    17281 gtcggacagc gggtcgcacc ccacgatctc gaacgacgtc tcccgcccgt ccgagaacga
    17341 cgcattgccc ccgtcggagc gaccgacgac gtgcgcggac gtgagcacga agccgtccga
    17401 cgtgatcacg acgccgctcc cgttgcccga catgcgccgg ccgcctcgca ccttgccgaa
    17461 gaccgcgagg ttcgtcaccg acggcgcgag cctctccgcg atgc
//
LOCUS       DASQDO010000352         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_343428, whole genome shotgun sequence.
ACCESSION   DASQDO010000352 DASQDO010000000
VERSION     DASQDO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343428"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="eno"
                     /locus_tag="VEV43_09900"
     CDS             complement(<1..841)
                     /gene="eno"
                     /locus_tag="VEV43_09900"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437673.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYP23876.1"
                     /translation="MSASIEFIQARQILDSRGNPTVEVDVVLEDGTLGRAAVPSGAST
                     GENEAVELRDDDKKVYLGKGVLKAVENVNSKIAPELIGLDPRDQESIDHLMIELDGTP
                     NKAKLGANATLAVSMAVAKASAELCNLPLYRYLGGNGAKTLPVPMLNILNGGKHADNT
                     VDFQEFMVQPWGFETYAEALRAGVEIYHALKGVLKKQKLSTAVGDEGGFAPDLKNNED
                     ALKIIEEAVGKAGYRWGEQVFVALDPAMSELWNEGEKLGKKGYCFFKSNPKEVLSSDD
                     VIGI"
     gene            complement(1140..1517)
                     /locus_tag="VEV43_09905"
     CDS             complement(1140..1517)
                     /locus_tag="VEV43_09905"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HYP23877.1"
                     /translation="MRHVYTGRDEMDAHFVKGLLEQEGIEAVVVGENLVSAVGTLPLS
                     AQSLPHVSVRDEDETRAAAVVEAYRQTDRANADDAVADAPRATWKCANCGEAVEEQFT
                     ECWKCGHARPASASTITPDDGPH"
     gene            complement(1564..>2454)
                     /locus_tag="VEV43_09910"
     CDS             complement(1564..>2454)
                     /locus_tag="VEV43_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23878.1"
                     /translation="WEVVRSEPILRGAADALGHTRRESFQVGPLLARHLEVTACPFPA
                     TDPQGLVLVVHDATESVRYQELRKEFVANVSHELRTPLTAIKGFAETLRDGAMTDPVR
                     GPQYLGTIEKHADQLTNLVADLLDLSRLDNHPGLPGSVAIDVGAVVRRTVDLLQPVAE
                     KKRHTLRVDVPPDLPPIQGDPDYLERAVANLLENAIKYTPEGGHVSIAASLAADEDVV
                     RVEVSDDGIGIPADDLPRIFERFYRVDRSRSREMGGTGLGLSIVKHVAQAHGGSIEVA
                     SDVGKGSTFTLTLPLTLPRP"
BASE COUNT          340 a          778 c          900 g          436 t
ORIGIN      
        1 agatgccgat cacgtcgtcg gacgagagga cttccttcgg gttgctcttg aagaagcagt
       61 agcccttctt gccgagcttc tcgccctcgt tccagagttc ggacatcgcg gggtcgaggg
      121 cgacgaagac ctgctcgccc caccggtagc cggccttgcc gacggcttcc tcgatgatct
      181 tgagcgcgtc ctcgttgttc ttgaggtccg gcgcgaagcc gccctcgtcg ccgacggcgg
      241 tggagagctt ctgcttcttc agcacgccct tgagcgcgtg gtagatctcc acgccggcgc
      301 ggagcgcctc ggcgtaggtc tcgaagcccc agggctggac catgaactcc tggaagtcga
      361 cggtgttgtc cgcgtgcttg ccgccgttga ggatgttgag catcggcacc ggcagcgtct
      421 tggcgccgtt gccgccgagg taccggtaga gcgggaggtt gcacagctcg gccgacgcct
      481 tggcgacggc cattgagacg gcgagggtgg cgttggcgcc gagcttggcc ttgttggggg
      541 ttccgtcgag ctcgatcatc aggtggtcga tcgactcctg gtcgcgcggg tcgaggccga
      601 tcagctcggg ggcgatcttc gagttcacgt tctcgacggc cttgagcacg cccttgccga
      661 ggtagacctt cttgtcgtcg tcgcgcagct cgacggcctc gttctcaccg gtgctcgcgc
      721 cgctcgggac ggccgcccgg ccgagggtgc cgtcttcgag gacgacgtcg acctcgacgg
      781 tcgggttgcc gcgggaatcg aggatctgcc gcgcctggat gaattcgatg ctcgcgctca
      841 tgtgtgatcc gacccggcgg aggcgctcgg gccgctcctt ctcggtgagg aaatcgtccg
      901 gactgaacag aacgtcagcc gcggcgcggc agtgtaagcg cgggggtggg atagtcaatt
      961 caggagacga tgtgacgcca agacgccaag gtgccaaggc aggagggagg agaagggcgg
     1021 aagacgtttg ccacgagcga acacgaatca gagggaactg cattcgtgtt tattcgtggc
     1081 tcgtctgctc ctcctctttc ctaactgggg cgcttggcgt cttggcgttc acatcgcctt
     1141 cagtgcggtc cgtcgtccgg ggtgatcgtc gaggccgacg cggggcgggc gtggccgcac
     1201 ttccagcact cggtgaactg ctcttcgacg gcttccccgc agttggcgca cttccaggtg
     1261 gcgcggggcg cgtcggcaac cgcatcgtcc gcattcgcgc ggtccgtctg gcggtaagcc
     1321 tcgacgacgg ccgcggcgcg cgtttcgtct tcgtcgcgga cggagacgtg gggcagggat
     1381 tgggcggaca gcggcaaggt gccgacggcg ctgaccaggt tctcgccgac gacgacggcc
     1441 tcgatgccct cctgctcgag caggcccttg acgaagtggg cgtccatctc gtcccgcccg
     1501 gtgtaaacgt gtcgcatggg gcccatggta cccgcagcga gcccgtgcgg ggaacgcacg
     1561 acgtcagggc cgcggcagcg tgagcggaag ggtcagcgtg aacgtggagc ccttgcccac
     1621 gtcgctggcg acctcgatcg atccgccgtg cgcctgcgcg acgtgcttga cgatggacag
     1681 gccgaggccc gtgccgccca tctcgcgcga gcgggagcgg tcgacgcggt agaagcgctc
     1741 gaagattcgc ggcaggtcgt cggcggggat gccgatgccg tcgtcgctca cctcgacgcg
     1801 cacgacgtcc tcgtccgccg ccaatgacgc cgcgatgctc acgtgcccgc cctcgggcgt
     1861 gtacttgatc gcgttctcca gcaggttggc gacggcgcgc tcgaggtagt ccgggtcgcc
     1921 ctggatcggc gggaggtcgg gcggcacgtc gacgcggagg gtgtgccgct tcttctccgc
     1981 gacgggctgg agcaggtcga cggtccgccg gacgacggcg ccgacgtcga tcgcgacgct
     2041 gccggggagg cccgggtggt tgtcgaggcg cgacaggtcg agcaggtcgg cgacgaggtt
     2101 ggtgagctgg tcggcgtgct tctcgatggt gccgaggtac tgggggcccc gcacggggtc
     2161 cgtcatcgcg ccgtcgcgga gcgtctcggc gaatcctttg atggcggtca gcggggtgcg
     2221 cagctcgtgg gagacgttgg cgacgaactc cttgcgtagc tcctggtagc gcacggattc
     2281 agtcgcatcg tgcacgacga gcacgagccc ctgcgggtcg gtggcgggaa acgggcacgc
     2341 cgtcacctcg aggtgccgcg cgagcagcgg cccgacctgg aagctctcgc gccgcgtgtg
     2401 gccgagcgcg tcggccgccc cgcgcaggat cggctcgctg cgcacgacct ccca
//
LOCUS       DASQDO010000353         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_833016, whole genome shotgun sequence.
ACCESSION   DASQDO010000353 DASQDO010000000
VERSION     DASQDO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833016"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..2907
                     /locus_tag="VEV43_09915"
     CDS             73..2907
                     /locus_tag="VEV43_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23879.1"
                     /translation="MTRRRILTWAIVGLAAASAVMLKDLGLLLGEIQTSGSRSYDASV
                     FTGPHLVFWDTGELQDAIEVWRLEREVVEGLLAWHFGADVIFALALGVVVWMALRASG
                     AQPEFARRAGRLYAALDVVENVVTLFVLRRLVGGTTPVGRSPGLVAIQFLALLKWAVL
                     LTAAVVAVVALFERLRDRPEGDRHRPVARARRGGVQAPAQALASLIVLVTLFVALVAL
                     PAGGPLDQIPDVIRQHLAEGGLGLWTLSTLGRMLFLAAVSVAGLAATSPAGRTAQDAT
                     IPDRVVLAVAAGASGLFALISVWRGSGLHKGLAAPVVVVGALWLAAAIARWAGVVPAA
                     PTPPREKPLTTWTDASILWVGGLAGAVAVAGSLGLVRAAFPPWVLGIDDGRWPWWAAA
                     TAGFLGSAVAGGATQAAVLFVARRSRRLHLVVTGVVVGSVVAVAAGLAVAPDWGASWG
                     STGVVGAGFGLWALVIGGLIWLSRTRPRWDVTRQVGFGARTPWFTLLLVVGLVGSSLG
                     KDAGYHDARLVAQAHVAQHPELDTVFSSWRGAVAEECAPQAGEPIPMVFVAAPGGGIR
                     AAYWTVSVLEKLVPGRCAATHLFAVSGVSGGSVGAATWVTARGLERSPTAAVTAMSED
                     VALPATLAGLLLRDVPQPLTGIADAWDDRAALLERGWADASGIFGSDDDPTRWSEAGD
                     GGGLAFVPLVMLNGSSVTDACRVVTGNVAGLPASTGPDCQGVPSPGNPVAGPETGTID
                     PFVGLRARGSDACGGGVDVPTTTAALLSARFPFVSPSGVIERCVETGSGKETISSNVV
                     DGGYYENSGLLTSLQLWAALDEEVADHNESNPANPVEPWFVLVDNHYRATAKKVPPGS
                     PMELLVPLEGFQQKVLTQTMLEQESVWEMTDAGGEPRFVYLAPTVRLGVEAPLGWVLS
                     SVSRDDLDTQLEEILEDPGPVLEELLDLLS"
     gene            2974..>3529
                     /locus_tag="VEV43_09920"
     CDS             2974..>3529
                     /locus_tag="VEV43_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HYP23880.1"
                     /translation="MLAKTLSVALIGADAHLVEVEVHTTQGVPNFTVVGLPAKSVREA
                     EQRTRSALLSSEQRWPPCRTVANLAPASLRKEGTHFDLAIAIGILAADKRLAPDQVEG
                     WVFLGELSLDGTLRPVRGALPAALAARKKGCRGIVCPAANAAEAALVDDLQVVAVPRL
                     VQAIAFLRGKWTPEPFEPIPRSEPT"
BASE COUNT          429 a         1234 c         1310 g          556 t
ORIGIN      
        1 gccgctaggt ccttgacttc catccgcacc ccggggttga gtgaacgcac ggttccgcgt
       61 gggggggtgg acatgaccag gcgcaggatt ctgacttggg cgatcgttgg cctcgccgcg
      121 gcgtccgcgg tgatgctgaa ggacctcggg ctcctcctgg gcgagatcca gacgagtgga
      181 agccgctcct acgacgcgag cgtcttcacc ggcccgcacc tcgtcttctg ggacacgggc
      241 gagctgcagg acgcgatcga ggtatggagg ctcgagcgag aggtcgtcga gggcctgctc
      301 gcatggcact tcggggccga cgtgatcttc gccctcgcgc tgggggtcgt cgtctggatg
      361 gccctgcgcg cgtccggcgc gcagccggag ttcgcgcggc gggcgggccg gctctacgcc
      421 gccctcgacg tcgtcgagaa cgtcgtgacg ctgttcgtcc tgcgacgtct cgtcggcggg
      481 acgaccccgg tcgggcggtc gccggggctg gtcgcgatcc agttcctcgc gctgctgaag
      541 tgggctgtcc tgctcacggc cgcggtcgtc gccgtcgtcg cgctgttcga gcgcctgcgc
      601 gaccgcccgg agggcgaccg gcaccgcccg gtcgcgcggg ctcggcgcgg gggcgtgcag
      661 gcgcccgctc aggccctcgc gtcgctcatc gtcctcgtga cgctgttcgt ggccctcgtc
      721 gcccttccgg ccggcgggcc gctcgaccag atccccgacg tcatcaggca gcacctcgcc
      781 gaggggggtc tcgggctctg gacgctctcg acgctcgggc ggatgctgtt cctcgcggcg
      841 gtgtcggtcg ccggactcgc agcgacgagc cccgcgggcc ggacggccca ggacgcgacc
      901 atccccgacc gtgtcgtgct cgcggtcgcg gccggagcgt cgggcttgtt cgctctgata
      961 tcggtgtggc gcggcagcgg tctgcacaaa ggcctggcgg cgccggtcgt agtcgtaggc
     1021 gctctttggc tcgcggccgc gatagcgcgc tgggccggcg ttgtgcccgc ggctccgacc
     1081 cctcctcggg agaagccgct gacgacctgg accgacgcgt cgatcctctg ggtcggcggc
     1141 ctggcggggg ccgtcgccgt cgcaggttcg ctcgggctgg tccgcgcggc gtttcccccg
     1201 tgggtcctcg gcatcgacga cgggcgctgg ccgtggtggg ccgccgccac ggcgggcttc
     1261 ctcggatcgg ccgtcgccgg aggagcgacg caggcggccg tgctcttcgt tgcccgccgg
     1321 tcccggcgtc tccatctcgt ggtcaccggc gtcgtcgtgg gctcggtcgt ggccgttgca
     1381 gccgggttgg ccgtcgcgcc cgactggggc gcgagctggg ggtccaccgg tgtcgtcggc
     1441 gcaggcttcg gcctgtgggc gctcgtgatc ggcggcctca tctggctctc tcgtaccagg
     1501 ccgcgctggg acgtcacgcg gcaggtcggc ttcggcgcgc gcacgccttg gttcactctg
     1561 ctgctcgtcg taggcctcgt cgggagctca ctcggcaagg acgcggggta tcacgacgcg
     1621 cgcctcgtgg cccaggcgca cgtcgcgcag cacccggagc tcgacacggt cttctcctcg
     1681 tggcgcggcg ccgtcgccga agagtgcgcg ccccaggccg gcgaaccgat cccgatggtg
     1741 ttcgtggctg cgccgggagg cgggatacgt gcggcgtact ggacggtgtc cgttctcgag
     1801 aagctggtcc cggggcgctg cgcggcgacg cacctgttcg ccgtgagcgg cgtctccggc
     1861 ggaagcgtcg gagccgcgac gtgggtgacg gcccgcggcc tcgagaggtc tccgaccgcg
     1921 gcggtcactg cgatgtccga ggacgtcgcg cttccggcga cgctggccgg cctcctgctg
     1981 cgcgacgtcc cgcagcctct gaccggcatc gcggacgcgt gggacgaccg ggccgccctg
     2041 ttggagcgcg ggtgggccga cgcgtccggc atcttcggct ccgacgacga cccgactcgg
     2101 tggagcgaag ccggtgacgg cggtgggctc gcgttcgtgc cgctcgtgat gctgaacggc
     2161 agctcggtca ccgacgcatg ccgggtggtg accgggaacg tcgcgggcct ccccgccagc
     2221 acgggccccg actgccaggg cgtcccctcg ccgggcaacc ccgtggccgg tcccgagacc
     2281 gggacgatcg acccgttcgt cgggctacgc gcccgcggct ccgacgcttg cgggggcgga
     2341 gtcgacgtcc cgacgaccac cgccgcgctg ctctccgccc ggttcccgtt cgtcagcccc
     2401 tcgggcgtca tcgagcgctg cgtcgagacc gggagcggga aggagacgat ctcgagcaac
     2461 gtggtcgacg gcggctacta cgagaacagc ggcctgctca cgtcgttgca gctgtgggcc
     2521 gcgctcgacg aggaggtcgc cgaccacaac gaaagcaatc cggcgaaccc ggtggagccg
     2581 tggttcgtcc tcgtcgacaa ccactaccgg gcgaccgcga agaaggtccc gccggggagc
     2641 ccgatggagc tcctcgtccc gctcgagggc ttccagcaga aggtcctgac gcagacgatg
     2701 ctcgagcagg agagcgtgtg ggagatgacc gacgccggtg gcgagccccg gttcgtctat
     2761 ctggcgccga cggtccggct aggggtcgag gcccccctcg gatgggtcct gtcgtccgtc
     2821 agccgagacg atctggatac gcagctcgaa gagatcctgg aggaccccgg ccccgtcctg
     2881 gaggagcttc tggacctcct ctcctgaagt tcttgtcaca gggggcagct agcttgtaat
     2941 cgggccgcga acccggggag agcgaggttt cgcgtgctgg cgaagacact gtccgtcgcg
     3001 ctcataggcg cggacgccca cctggtcgag gtcgaggtcc acacgaccca gggcgtcccg
     3061 aacttcacgg tagtcgggct cccggcgaaa tccgtccgcg aggccgagca acgaacccgc
     3121 tcggcgctgc tgtcgagcga acaacgctgg cccccctgcc ggacggtggc gaacctagcc
     3181 ccggcctccc tgcgcaaaga aggaacgcac ttcgacctgg cgatagcgat aggcatcctc
     3241 gccgccgaca agcgcctggc gcccgaccag gtggaggggt gggtcttcct cggcgagctc
     3301 agcctcgacg gcacgctgcg cccggttcgc ggcgcgcttc cggcggccct cgccgcgcgg
     3361 aagaaaggat gtagggggat cgtgtgcccg gctgcgaacg cggccgaggc agctctggtc
     3421 gacgatctcc aggtcgtcgc cgtgccgagg ctcgtgcagg cgatcgcctt cctgcggggc
     3481 aagtggacgc cggagccgtt cgaaccgata ccgcgctcgg agccgaccg
//
LOCUS       DASQDO010000354         9813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_833193, whole genome shotgun sequence.
ACCESSION   DASQDO010000354 DASQDO010000000
VERSION     DASQDO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833193"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VEV43_09925"
     CDS             complement(<1..274)
                     /locus_tag="VEV43_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23881.1"
                     /translation="MNKAGLGATLAFLTGVAGIFLWVSMLAGPFRLASGLLDARDHLQ
                     RAERALSNASTKAARYETLAAVAATERARTGLDSKSPLRSLAEKAPV"
     gene            complement(277..1311)
                     /locus_tag="VEV43_09930"
     CDS             complement(277..1311)
                     /locus_tag="VEV43_09930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYP23882.1"
                     /translation="MAFKIGVGLFTGQIPRGSDRSFHTDYRDAIDLVQLAEEEGFDSA
                     WVSEHHGSEDGYLPSLLPLLAAFAARTSRIELGTGVVLAPFHDPLRLAEDFAVVDQIS
                     GGRVVCGLGIGWREEEFRAFGIDVSTRVRRLVEIVEILRRAWTGERFDYDGRHYSYEG
                     VAVTPAPAGVPPIYVGGFADTAIRRAGRIGDGYISSRAQPDRVAEAFEMAAAARSKAG
                     REGKPLAALLQNAFVTSDPARDWPLVAAGIGHQLGVYAGWRAGTDVPGTPLEVVPPDE
                     GTIRRTTAFGTPDEVCAYLEPLVRVLAPHPDSHLILRFHYPGMDAAPAAESIRLFARE
                     VAPRLRAAAA"
     gene            complement(1384..2076)
                     /locus_tag="VEV43_09935"
     CDS             complement(1384..2076)
                     /locus_tag="VEV43_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23883.1"
                     /translation="MRKLGSTLIAGAFLFGLIGNAYAGKPVTVFEDPANDAGNTEQGV
                     ALPPATAAGLDLISGQVAKSGADLEFTVTNAAGLPQAGSAPEGFRLLWHINSGGEEFR
                     FTVKSLDVGKPDAIAQEGTDRIGQVYQGVARLETCADQALPAVLTLVNCKGVEYFQAT
                     FDPAAATVTWKVPLASIKAKTGTLIAGGTTAAASSNCQICWVPHYAERSLTPSTIIDN
                     ATMVTSYKVPKK"
     gene            complement(2143..3312)
                     /locus_tag="VEV43_09940"
     CDS             complement(2143..3312)
                     /locus_tag="VEV43_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23884.1"
                     /translation="MRRIVCLAAALLMAVAGVAQAAPEGAADLGPSAGGISSDNVEFI
                     SNIPISVDGVGGRVIGNYFYTNDQNKVMIFDISDPLNPEMTGFVPMPQEVLYSREDLD
                     GNGDILVVPNTVIPNEQEREAGKLIGATYIIDVEDKTNPTILAELPGSAQHTMSCILK
                     CKWAYGSDGNIIDLRDPAKPKLIEKKWGEGLPAQSGHDVTEVSNGRVLTSTQPMMLLD
                     VRKPTAPKLLASGMNLDGRFVHSNVWPQGGKDKFFLAGGETNNQVRCNERNGAFMTWD
                     ASKWKKTKTFTMIDEYRMVNGTYVDGSPAVNAVGCSSHWLEAHPTFKDGGVVAAAFFE
                     HGTRFLDISSKGQIEEIGWFMPHGGSTGASYWITDEIVYSVDYTRGIDILRFTDK"
     gene            complement(3387..4277)
                     /locus_tag="VEV43_09945"
     CDS             complement(3387..4277)
                     /locus_tag="VEV43_09945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYP23885.1"
                     /translation="MRILFLGGGRPAEELLGAAAADASVETTTGLGETLRRLDEAEYD
                     VVVVDLTSRGRLGGTAERDGSIDDPVPAIGGAAPRTPVVVLTGAGDEAEGLRAVRAGA
                     QDDVALDAPPEGQLARRLRYAIERKRREQALARHAHYDPLTDLANRALFRERLAQALA
                     RADRTGKVVAVLWLDLDGFKEATAGWGREGSDELIRQAGVRLHRSVRQVDTVGRPAGD
                     EFTILLEGLGGPEEAEVVARKVATALKEPLLKDPETTLSGTIGIAWYPQSPAGEVLQH
                     AFEALRTAKRTAKGSFRFHE"
     gene            complement(4283..6259)
                     /locus_tag="VEV43_09950"
     CDS             complement(4283..6259)
                     /locus_tag="VEV43_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HYP23886.1"
                     /translation="MERRERQRPWIFRTYAGYASPDATNERFKSNLAKGQTGLSVAFD
                     LPTQTGYDSDHPMARGEVGKVGVPISHLGDMKTVFDGIPLAEMNTSMTINAPAVWLLA
                     LYVATADDQGAGRGSLAGTTQNDVLKEYLSRGTHIFPPDASIRLTADLITWTLHEIPK
                     WNPINVCSYHLQEAGAGPVLEVAFALANAVAVLDAVRATGSVSDEDFPRLVGRISFFC
                     NAGIRFVEETCKMRAFGRMWGRIAEQRYGVEEERYRRFRYGVQVNSLGLTAAQPENNV
                     YRILLEMLSVTQSKDARARAVQLPAWNEALGLPRAWDQQWSLRAQQILALETDLLEYD
                     DLFAGSHVVESLTDEIEAGATEELERILDAGGSVEALGYMKERLVAAHAERRRAIESG
                     EETVVGVNAYTETEPSPLVEGLDVMAFEKVDPAAEAAQVERLQEWRERRDAGDVRGAL
                     DALRRAAENGDNLLPPSIEAAHAGVTTGEWADALRDVFGEYRAPTGVTANVTAGATGI
                     ERAQRAVAEAVARTGAAKLRMLVAKPGLDGHSNAAEQVAVRARDAGFEVVYQGIRLSP
                     EQIARAAAEEDVDVVAISILSGAHNLLVPDVIDRLRAEGVDPLAVPVIVGGVIPDDDA
                     AKLAELGVARVYTPRDHDMTRMIAEIADLIPARS"
     gene            6284..8050
                     /locus_tag="VEV43_09955"
     CDS             6284..8050
                     /locus_tag="VEV43_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HYP23887.1"
                     /translation="MSLQIKKVGVVGCGTMGSGICEVCARAGYEVVFTEITQEAVEAG
                     RDRIARSLRRGVERGKMDQAEADSILSRISSSTNNGDLADCDVVFEAVPEHLDLKKKV
                     FAELDEILGPDALLATNTSSLPVIDMAVATRRPSKVIGFHFFNPAQVMKLVELVHTVA
                     TEEDSLAAAREFAEGLGKTPVPCRDRAGFIANLLLFPYLNSAVRMFDQGYASREDIDA
                     AMQLGCGHPMGPLALLDLIGMDSGYEICEALWRQFRDDQDAPAPLLKQYVAAGYLGRK
                     TGRGFYKYASPESSKVVDGIPDEAGVATSDVTTVGIVGTGLMASGIAEVAAKAGNEVI
                     LRGRSEAAIKRAKAAVAKSLDRAVSKGKLDQAAADDVLGRITTTLEFSDLAEVDLVVE
                     SVAEDLPVKDAIFRELDAATKPEAILATTTSSLSVVDLAAATARPDRVVGLHFFNPAP
                     VMKLVEVMRTVATSGEAEKRAVAWARSLGKRPVRCRDRAGFIVNFLLFPYLNDAVRMH
                     EEGYGTPQDIDIAMKLGCGHPMGPFELMDIVGLDVTNAILNSLHEEFRERRHAPAPLL
                     EHMVRAGYLGRKAGRGFYDYSQ"
     gene            8150..8908
                     /locus_tag="VEV43_09960"
     CDS             8150..8908
                     /locus_tag="VEV43_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP23888.1"
                     /translation="MTERVVVVTGGGSGLGLAIAERVAAGGATVAIAEIDPARGKEAA
                     ERLGGEAFAVECDVADLDSVRSAVSAVVKRAGGIDALVNNAGFDLPGFFLQTDPGMWR
                     SLIAVNLEGVLNCIYAAAPVLIERAAQSGYGRIVNVASDAGRVGSSGEAVYSAAKGGV
                     IAFGKSMARELARHGITVNSVCPGPADTPMTAAIRQNEMGEKMFQKLMAATPLKRLVQ
                     PAEVAEAVAYFLSDEAAFTTGQTLSLSGGLTMSG"
     gene            8974..9351
                     /locus_tag="VEV43_09965"
     CDS             8974..9351
                     /locus_tag="VEV43_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23889.1"
                     /translation="MLRSRLIVPFALAAVVATAVPAGAVEVKPEAALPTCQSAFSMRV
                     TEYHETHYMTGTAAVPGATEVRLTCGLVRWGETVATISDRTTGPVAVVAGSVTIMGAL
                     HDICYVIQAWYPDGTYRIQDTCP"
     gene            complement(9359..9661)
                     /locus_tag="VEV43_09970"
     CDS             complement(9359..9661)
                     /locus_tag="VEV43_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23890.1"
                     /translation="MSSMYRPPRRERRFEIVSIDGNATEPERRAIEAALDHSLEAERA
                     SREPSLWLRSGRAQGRRLGMYDYRDRFAHEDAWRLSLRMPSGGREYPGLNGRGDAK"
     gene            complement(9658..>9813)
                     /locus_tag="VEV43_09975"
     CDS             complement(9658..>9813)
                     /locus_tag="VEV43_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYP23891.1"
                     /translation="RFGNPYIAAERGYIDDVIEPQDTRRRLVQSLEMLRSKRETIPQR
                     KHGNVPL"
BASE COUNT         1379 a         3433 c         3459 g         1542 t
ORIGIN      
        1 cgacgggcgc cttctccgcc agggaccgca gcgggctctt cgaatcgagt ccggtgcggg
       61 cccgctcggt cgcggcgacc gcggccagcg tctcgtagcg cgcggccttc gtcgacgcgt
      121 tcgagagcgc gcgctcggcc ctctggaggt ggtcgcgcgc gtcgagcaag ccacttgcga
      181 gacggaaagg cccggcgagc atcgagaccc acaggaagat cccggcgacc ccggtgagga
      241 acgcgagcgt cgcgccgagc ccggccttgt tcatcgtcag gccgccgcgg cgcgcaagcg
      301 cggggcgacc tcgcgcgcga acagacggat cgactccgcg gcgggggccg cgtccatccc
      361 ggggtagtgg aaccgcagga tcaggtgcga gtcgggatga ggggcgagca cccgcaccag
      421 cggctcgagg tacgcgcaga cctcgtcggg ggtcccgaac gcagtcgtgc ggcggatcgt
      481 gccttcgtcc ggcgggacga cctcgagggg cgtccccggc acgtccgtcc cggctcgcca
      541 ccccgcgtag acgccgagct ggtgcccgat gcccgcggcg acgagaggcc agtcgcgcgc
      601 ggggtcgctc gtgacgaacg cgttctggag caacgccgcc agcggcttcc cctcccgccc
      661 cgccttgctc cgggcggccg cggccatctc gaacgcctcc gcgacgcggt ccggctgcgc
      721 ccgggacgag atgtagccgt cgccgatgcg cccggccctc cggatagcgg tgtcggcgaa
      781 ccctccgacg tagatcgggg ggacgcccgc gggggccggg gtgacggcga ccccttcgta
      841 cgagtagtga cggccgtcgt agtcgaagcg ctcgccggtc caggcgcggc gcaggatctc
      901 gacgatctcg acgagcctgc ggacccgcgt cgacacgtcg atgccgaagg cgcggaactc
      961 ctcctcccgc cagccgatcc cgagcccgca cacgacgcgg ccaccggaga tctggtcgac
     1021 gacggcgaag tcctccgcca gccgcagcgg gtcgtggaac ggggcgagca cgacgccggt
     1081 gccgagctcg atgcgcgacg tgcgcgcggc gaacgcggcg agcagcggca gcagcgacgg
     1141 gaggtagccg tcctcggacc cgtggtgctc ggacacccac gccgagtcga agccctcttc
     1201 ctcggcgagc tgcacgaggt cgatcgcgtc gcggtagtcc gtgtggaacg acctgtccga
     1261 gccgcgcggg atctgccccg tgaacaggcc cacgccgatc ttgaaagcca cgcgcccctc
     1321 ccgccggaaa aaatcaggag cccccgaagg ggctcctgag gaagataccg gaagggtgtg
     1381 gctttacttc ttggggacct tgtagctggt caccatcgtc gcgttgtcga tgatcgtgct
     1441 cggggtgagc gagcgctcgg cgtagtgggg gacccagcag atctggcagt tcgacgaagc
     1501 tgcggccgtc gtgccaccgg cgatgagcgt gccggtcttg gccttgatcg acgccagggg
     1561 gaccttccac gtgaccgtcg cagcggccgg gtcgaacgtc gcctggaagt actcgacgcc
     1621 cttgcagttg acgagcgtca ggaccgccgg gagcgcctgg tcggcacacg tctcgagacg
     1681 ggcgacgccc tggtagacct ggccgatccg gtcggtgccc tcctgggcga tcgcgtcggg
     1741 cttgccgacg tcgaggctct tgaccgtgaa gcggaactcc tcgccacccg agttgatgtg
     1801 ccacaggaga cggaagccct cgggggcgct gccggcctgc gggaggcccg cggcgttcgt
     1861 gaccgtgaac tcgaggtcgg caccggactt ggcgacctga ccgctgatca ggtcgaggcc
     1921 tgccgccgtc gccggcggga gggctacgcc ctgctcggtg ttgcccgcgt cgttcgccgg
     1981 gtcctcgaac accgtcacgg gcttgcccgc gtaggcgttg ccgatcaggc cgaacaggaa
     2041 cgctcctgcg atgagggtgc taccgagctt gcgcattgtc gttggctcct ggttggaagt
     2101 tgtcgttgag ctctgggttc tccgcgcccg ggggcggtcc tgctacttgt cggtgaagcg
     2161 gaggatgtcg atgccccggg tgtagtcgac ggagtagacg atctcgtcgg tgatccagta
     2221 cgacgcaccg gtcgagccgc cgtgcggcat gaaccagccg atctcctcga tctgaccctt
     2281 cgacgagatg tcgaggaagc gcgtgccgtg ctcgaagaac gctgccgcga cgaccccgcc
     2341 gtccttgaag gtggggtgcg cctcgagcca gtgcgacgag cagccgactg cgttgaccgc
     2401 aggcgacccg tcgacgtacg tgccgttcac catgcggtac tcgtcgatca tcgtgaacgt
     2461 cttggtcttc ttccacttcg acgcgtccca cgtcatgaaa gcgccgttgc gctcgttgca
     2521 gcggacctgg ttgttggtct cgccgccggc gaggaagaac ttgtccttgc cgccctgcgg
     2581 ccagacgttc gagtgcacga agcggccgtc gaggttcatg cccgaggcga gcagcttcgg
     2641 cgccgtcggc ttgcggacgt cgaggagcat catcggctgg gtcgacgtga gcacacggcc
     2701 gttcgagacc tcggtgacgt cgtgaccgct ctgggcggga aggccctcgc cccacttctt
     2761 ctcgatgagc ttgggcttcg ccgggtcgcg caggtcgatg atgttgccgt ccgagccgta
     2821 cgcccacttg cacttgagga tgcagctcat cgtgtgctgg gcggagccgg gcagctcggc
     2881 gaggatcgtc gggttcgtct tgtcctcgac gtcgatgatg taggtggcgc cgatgagctt
     2941 gccggcctcc cgctcctgct cgttcgggat gaccgtgttg ggcacgacga ggatgtcgcc
     3001 gttcccgtcg aggtcctcac gcgagtacag gacttcctgc ggcatcggga cgaacccggt
     3061 catctcgggg ttcagcgggt ccgagatgtc gaagatcatg accttgttct ggtcgttcgt
     3121 gtagaagtag ttgccgatca cacggccccc gaccccgtcg acggagatcg ggatgttcga
     3181 gatgaactcg acgttgtccg acgagatgcc gccggccgac ggtccgaggt ccgcggcccc
     3241 ctcgggcgcc gcctgggcaa cgccggctac cgccatcagc aaggcggccg ctaggcacac
     3301 gattctccgc actgggatcc cctccgtcgg tctagttcgt tggttgtcgt gctctaggtt
     3361 cgaccgcgga ccgggaatcc ctctcgtcac tcgtggaagc ggaagctccc cttcgccgtc
     3421 cgcttcgccg tacgcagcgc ctcgaatgcg tgctggagga cctcgccggc cgggctctgc
     3481 ggataccagg cgatgccgat cgtccccgag agcgtcgtct cggggtcctt gaggagcggc
     3541 tccttcaggg ccgtcgcgac cttgcgggcc actacctcgg cctcctccgg gcccccgagc
     3601 ccttcgagca ggatcgtgaa ctcgtcgccc gcgggccgcc cgacggtgtc gacctggcgg
     3661 accgagcggt gcagccggac gccggcttga cggatcagct cgtcggaccc ctcgcggccc
     3721 cagccggccg tcgcctcctt gaacccgtcg aggtcgagcc acagcaccgc cacgaccttc
     3781 cccgtgcggt cggcgcgggc gagggcctgg gcgagccgct cgcggaacag cgcgcggttc
     3841 gctaggtccg tcagcgggtc gtagtgcgcg tggcgggcga gcgcctgctc gcgcctcttg
     3901 cgctcgatcg cgtagcggag cctccgggcg agctgccctt ccgggggggc gtcgagcgcg
     3961 acgtcgtcct gcgccccggc cctgaccgcc cgcagccctt cggcctcgtc gccggcgccg
     4021 gtgaggacga cgaccggggt cctgggggcc gcgccgccga tcgccgggac ggggtcgtcg
     4081 atcgagccgt ctcgttcggc ggtcccgccg aggcggcccc ggctggtcag gtccaccacg
     4141 acgacgtcgt actcggcctc gtcgagccgc cgcagggtct cgccgagccc ggtcgtggtc
     4201 tcgacgctcg cgtccgcggc tgcggccccg aggagctcct cggccgggcg gccgccgccg
     4261 aggaacagga tcctcatgtt cgtcaggagc gggccgggat gaggtccgcg atctcggcga
     4321 tcatccgcgt catgtcgtgg tcgcgcggcg tatagacgcg ggcgaccccg agctcggcga
     4381 gcttcgccgc gtcgtcgtcg gggatgaccc cgccgacgat caccgggacg gccagcgggt
     4441 cgacgccctc ggccctcagc cggtcgatca cgtcgggcac tagcaggttg tgcgcccccg
     4501 acaggatcga gatcgcgacg acgtcgacgt cctcctcggc ggcggcgcgc gcgatctgct
     4561 cggggctcag cctgatcccc tggtagacga cctcgaaccc cgcgtcccgg gcccgcacgg
     4621 cgacctgctc ggcggcgttc gagtggccgt cgagccccgg cttcgccacg agcatgcgga
     4681 gcttcgccgc tccggtgcgt gccaccgcct cggctacggc tcgctgcgct cgttcgatcc
     4741 cggtggcgcc cgcggtgacg ttcgcggtca cgccggtcgg ggcgcggtac tcgccgaaga
     4801 cgtcgcgcag ggcgtctgcc cactcgccgg tcgtcacgcc ggcgtgcgcg gcctcgatcg
     4861 acgggggcag gaggttgtca ccgttctccg cggcccgccg cagcgcgtcg agcgcgcccc
     4921 ggacgtctcc ggcgtcccgg cgttccctcc actcctgcag gcgctcgacc tgagcggcct
     4981 cggccgcggg gtcgaccttc tcgaacgcca tcacgtcgag cccctcgacg agcggcgacg
     5041 gctccgtctc cgtgtacgcg ttgacgccga ccacggtctc ctcgccgctc tcgatcgcgc
     5101 gcctccgctc ggcatgggcc gcgaccagcc gctccttcat gtagccgagg gcctcgaccg
     5161 agccgccggc gtcgaggatc cgctcgagct cctcggtcgc tccggcctcg atctcgtcgg
     5221 tgagcgactc gaccacgtgc gacccggcga acaggtcgtc gtattcgagg aggtcggtct
     5281 cgagcgccag gatctgctgc gcgcgcagcg accactgctg gtcccacgcc cgcgggagcc
     5341 cgagggcttc gttccacgcc ggcagctgga cggctcgtgc ccgggcgtcc ttcgactggg
     5401 tcacagagag catctcgagc aggatccgat acacgttgtt ctccggctgc gccgcggtga
     5461 gcccgagcga gttcacctgc acgccgtagc ggaaccggcg gtagcgctct tcctccacgc
     5521 cgtatcgctg ctcggcgatg cggccccaca tccggccgaa cgcgcgcatc ttgcacgtct
     5581 cctcgacgaa gcggatgccg gcgttgcaga agaacgagat gcgcccgacg agccgcggga
     5641 agtcctcgtc gctcaccgag ccggtcgccc gcaccgcgtc gagcacggcg acggcgttcg
     5701 cgagcgcgaa ggcgacctcg agcaccggcc cggcccccgc ctcctggagg tggtacgagc
     5761 acacgttgat cgggttccac ttcgggatct cgtgaagcgt ccacgtgatc aggtccgcgg
     5821 tcagccggat cgaagcgtcg ggcgggaaga tgtgcgtccc gcgcgagagg tactccttca
     5881 ggacgtcgtt ctgcgtcgtc ccggccagcg acccgcgccc ggccccctgg tcgtcggcgg
     5941 tcgcgacgta cagcgcgagc agccacaccg ccggcgcgtt gatcgtcata gaggtgttca
     6001 tctcggcgag aggtatgccg tcgaagaccg tcttcatgtc gccgaggtgc gagatcggga
     6061 cgccgacctt tccgacctcg cccctcgcca tcgggtggtc ggagtcgtag ccggtctggg
     6121 tcgggaggtc gaacgcgacc gagagcccgg tctggccctt ggcgaggttc gacttgaacc
     6181 gctcgttggt cgcgtcgggg ctcgcgtagc cggcgtaggt ccggaagatc caaggtctct
     6241 ggcgctcgcg tcgctccatg agcgcagttt aggctcgctc cctatgagtc tccagatcaa
     6301 gaaggtgggc gtcgtcggct gcgggaccat gggttccggc atctgcgagg tctgcgcccg
     6361 cgccggctac gaggtcgtct tcaccgagat cacgcaggag gccgtcgagg cgggccgcga
     6421 ccggatcgcc cgcagcctcc gccgcggcgt cgagcgcggc aagatggacc aggccgaggc
     6481 cgactcgatc ctgtcccgca tctcgtcgtc gacgaacaac ggggacctgg cggattgcga
     6541 cgtcgtgttc gaggcggtgc ccgagcacct cgacctcaag aagaaggtct tcgccgagct
     6601 cgacgagatc ctcggccccg acgcgctcct cgcgaccaac acgtcttcgc tcccggtgat
     6661 cgacatggcg gtagcgacga ggcgtccgtc gaaggtcatc ggcttccact tcttcaaccc
     6721 ggcgcaggtg atgaagctcg tcgagctcgt ccacaccgtc gcgacggagg aggactcgct
     6781 ggccgcggcc cgcgagttcg ccgagggcct cggcaagaca ccggtgccgt gccgcgaccg
     6841 cgccgggttc atcgccaacc tgctgctgtt cccgtacctc aactcggccg tccgcatgtt
     6901 cgaccagggc tacgcgtcgc gcgaggacat cgacgcggcg atgcagctcg gctgcggcca
     6961 cccgatgggc cccctcgcgc tgctcgacct catcgggatg gactccggct acgagatctg
     7021 cgaggccctg tggcgccagt tccgcgacga ccaggacgcc ccggcccccc tcctgaagca
     7081 gtacgtcgcc gcgggctacc tcggccgcaa gaccggccgc ggcttctaca agtacgcgtc
     7141 gcccgagtcg agcaaggtcg tcgacgggat accggacgaa gccggggtcg cgacgtccga
     7201 cgtcacgacg gtcggcatcg tcgggaccgg cctgatggct tccgggatag cggaggtcgc
     7261 cgccaaggcc ggcaacgagg tcatcctgcg cggccgcagc gaggcggcga tcaagcgcgc
     7321 caaggccgcc gtggcgaaga gcctcgaccg cgccgtcagc aagggcaagc tcgaccaagc
     7381 cgccgccgac gacgtcctcg gccgcatcac gacgacgctc gagttctccg acctcgccga
     7441 ggtcgacctc gtggtcgagt ccgtagccga ggacctgccg gtcaaggacg cgatcttccg
     7501 cgagctcgac gcggccacca agcccgaggc gatcctcgcc accacgacgt cgtcgctctc
     7561 ggtcgtcgac ctggccgccg ccacggcccg gcccgaccgg gtcgtcggcc tccacttctt
     7621 caacccggcc cccgtgatga agctcgtcga ggtcatgcgc accgtcgcca cctcgggcga
     7681 ggccgagaag cgggcggtcg cgtgggcacg ctcgctcggc aagcggccgg tgcgatgccg
     7741 cgaccgggcc ggcttcatcg tgaacttcct tctgttcccc tacctgaacg acgccgtgcg
     7801 catgcacgag gagggctacg ggacgccgca ggacatcgac atcgcgatga agctcggctg
     7861 cggacacccg atggggccgt tcgagctgat ggacatcgtc ggcctcgacg tgacgaacgc
     7921 gatcctgaac tcgctgcacg aagagttccg ggagcgccgg cacgccccgg cccccctgct
     7981 cgagcacatg gttcgcgccg ggtacctcgg ccggaaggcg ggccgcggct tctacgacta
     8041 ctcccagtag ccctggttcg tgtggttggc aaggttggtg gggttgggtt tccaaccaca
     8101 cgtaccagac gtaccagacg taccagagct ctcgagaggt aggtactagg tgaccgagcg
     8161 agtcgtcgtc gtaacggggg gcgggtcggg gctcggcctc gccatcgccg agcgggtcgc
     8221 agccggcggc gcgaccgtcg cgatcgccga gatcgacccc gcccgcggca aggaggcggc
     8281 cgagcgtctc ggcggcgagg cgttcgcggt cgagtgcgac gtcgccgacc tcgactcggt
     8341 ccggtccgcc gtctcggccg tggtcaagag ggccgggggg atcgacgccc tcgtgaacaa
     8401 cgcggggttc gacctgccgg ggttcttcct ccagaccgac ccggggatgt ggcggtcgtt
     8461 gatcgcggtg aacctcgagg gggtcctgaa ctgcatctac gcggccgcgc cggtgcttat
     8521 cgagagggcc gcgcagagcg gttacggacg gatcgtgaac gtcgcctcgg atgcgggccg
     8581 ggtggggtcg tccggcgaag cggtgtactc ggcggcaaag gggggcgtga tcgcgttcgg
     8641 gaagtcgatg gcgcgggagc tcgcccggca cgggatcacg gtgaactcgg tctgcccggg
     8701 cccggccgac acgccgatga ccgctgcgat ccggcagaac gagatgggcg agaagatgtt
     8761 ccagaagctg atggcggcga cgcccctgaa gcgcctggtg cagccggccg aggtagccga
     8821 ggccgtcgcc tacttcctgt cggacgaagc agctttcacg acgggtcaga cgctgagcct
     8881 gagcggtggc ctgacgatgt ccggctagga ggtatcgggg ggccgggatc gaagcctggc
     8941 gggaccgttt cccggatcca gaggaggagc ccagtgctgc gctctcgcct gatcgtcccg
     9001 ttcgccctcg cggccgtcgt cgcaaccgcc gttcccgccg gcgccgtcga ggtcaagccc
     9061 gaggccgcgc tcccgacgtg ccagtcggcg ttctcgatga gggtcaccga gtaccacgag
     9121 acccattaca tgacgggcac ggcggccgtc ccgggcgcca ccgaggtgcg tctgacgtgc
     9181 ggcctcgtgc gctggggcga gacggtggcg acgatctccg accgcaccac cggcccggtt
     9241 gcggtcgttg cgggctcggt cacgatcatg ggcgcgctgc acgacatctg ctacgtgatc
     9301 caggcctggt acccggacgg cacctaccgg atccaggaca cctgccccta agggctagct
     9361 acttcgcgtc gccccggccg ttgaggccgg ggtactcgcg gcccccggac ggcatccgca
     9421 gcgacagccg ccacgcgtct tcgtgggcga agcggtccct gtagtcgtac atgcccagac
     9481 gacggccctg cgcgcggccc gaccgcaacc acagcgacgg ctccctcgat gcccgctcgg
     9541 cctcgaggga gtgatccagc gcggcctcga tcgcccggcg ctcgggctcg gtcgcgttgc
     9601 cgtcgatcga gacgatctcg aagcggcgct cgcgacgggg ggggcggtac atcgagctca
     9661 cagcgggacg ttgccgtgct tgcgctgggg gatcgtctcg cgcttcgagc gcagcatctc
     9721 gagcgactgc accaaccgtc gcctggtgtc ctgcggctcg atgacgtcgt cgatgtagcc
     9781 gcgctcggcc gcgatgtacg ggtttccgaa ccg
//
LOCUS       DASQDO010000355         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_894762, whole genome shotgun sequence.
ACCESSION   DASQDO010000355 DASQDO010000000
VERSION     DASQDO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894762"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1762)
                     /locus_tag="VEV43_09980"
     CDS             complement(8..1762)
                     /locus_tag="VEV43_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP23892.1"
                     /translation="MRRTHVSVRGWRRRVAVVCVVAAACSTASQATGDFPGGTSGTAL
                     APTATEPPIALRDRPAWLESACSLPLEHLQRIKRGYYRGRSPDVFLVPRQPHFFGRFF
                     STTHSGPWEYVQEVPLIFYGPGFVEAQGTLDLRRKITLADLAPTMARLLRMGWEKGRA
                     GRAIKEALLPDAQRPRSPKLIVQIVWDGGGWNVLNRWPRAWPHLASLIEGGTSVAGAM
                     VGSSPSVTPAIHSTMGTGEFPRRHGVVDIYMRNAKGEVKGALNGQPPHELELTTVGDL
                     FDRRNGNRPKVGALTYRYFHLGMMGHGAMLPGADRDVVAIAAREAGDRVTGPGRLITN
                     PDLYKLPYYLDDVPGWEEDVRSVDVEDGKLDGKWMRHADLTDPTTVRHSPVWTLYQTR
                     LIKELLAREDFGANRVPDLFFTNFKQIDDVGHDYNMVEPQMREILEYSDAALEDLTKW
                     LDRKVGEEQWVMVFTADHGQQPSAQALDAWPIGITPLQKDIARRFGVSYERLFEGERP
                     VGVWVDRDVQRAEDITLAEISEFVLGYRIGDNMETTEGVPEQYFERRRERLFDAAFPA
                     KKMGKVWDCAVARNGGAA"
     gene            complement(1738..3108)
                     /locus_tag="VEV43_09985"
     CDS             complement(1738..3108)
                     /locus_tag="VEV43_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYP23893.1"
                     /translation="MQRVRLALLALCWVGVVVAGVYVAEPASSQDDRPNVLVIVTDDE
                     RERDQMLVMPETLEIFGRGGTQFTHAFASTPQCCPSRASIMTGRYAHNHGVRRNQDGP
                     KMNQMVTMQRYLHERAGYKTALVGRFLNSWDVTQSPRFFDRWALFRAGYYNNEFNVNG
                     EMVEVQEYATDYIGKQSMEFLEEFDAEDDAAPWLMYVTVSAPHFPFFSSDEYAEAPVP
                     SWPDNPAITEDRLGDKPGFVHNVGFSAEQGEAIYHKQLRTLMSADDMVGSLFDKLEEL
                     DEDQETLAFFISDNGMMLGEHGLYAKRLPYTESIQVPFFVRWPGRVEAGGVDDRLVGN
                     IDIAPSVLDVAGLLPEVQTEVDGRSLFGPLERSRMLTEQWGSWSANRPTWASIRTHDY
                     QYIQYYFKDGRTRYREFYDLAADPWQTSNLLGDANPDNDPSPFVLKQLETQLETDRAC
                     AGLTCP"
     gene            complement(3116..4360)
                     /gene="hutI"
                     /locus_tag="VEV43_09990"
     CDS             complement(3116..4360)
                     /gene="hutI"
                     /locus_tag="VEV43_09990"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HYP23894.1"
                     /translation="MTASLTIAGGTHVTWAGTGPVRAEALAAPDVTEDGVLSARDGRF
                     AGGETAAEVTVDASGCVVVPGFVDCHTHLPFFGWRADEDAARLSGVRYETLHGREGGI
                     FRSARLLGEASDDEVLGFSGELAGEMLASGTTTFETKSGYGLSVEAELRQLGLARALA
                     GRVPQKVVTTCLAAHAVPKGRSEGEWIGIAAGQLLPEAAAGGLATACDLYVETIAFAL
                     EHAARLHETARGLGMRMRVHADQLSDGQTAAFAARWGFDTADHLNHSAPDAVGDLAQS
                     ETVAVLLPGATFTLRQSKKPPARDLLSRGAIVALGSDLNPGTSPVRSMPFVIALACRV
                     YGFRPAEALAAATVNAAAALGLEDEVGRLAPGYRADAVVLDVPSLDHLAYRPDRNPVA
                     AVVCGGEVVHVADGFEGRVKKG"
     gene            complement(4357..>4864)
                     /locus_tag="VEV43_09995"
     CDS             complement(4357..>4864)
                     /locus_tag="VEV43_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP23895.1"
                     /translation="AVVHTPGHARSHVCLWNASDGLLISGDHLLPTITPHIDFERGGE
                     EDPLGEFLESLEKVEDLDPKMVLPGHGRPFLEGAERARVIARHHDRRLGNILQVIRRE
                     PRSATTIVDEVFGSQLLHFERRLALGEALAHLAYLRQRGEVERVEADDGTFLYKKVSR
                     RRVVEVDD"
BASE COUNT          691 a         1734 c         1692 g          747 t
ORIGIN      
        1 gttcctccta ggcggcgcct ccgttgcggg ccaccgcgca gtcccagacc ttccccatct
       61 tcttcgccgg gaaggcggcg tcgaagagcc tctcgcggcg ccgctcgaag tactgctccg
      121 gcacgccctc ggtcgtctcc atgttgtcgc cgatgcgata gccgagcacg aactccgaga
      181 tctccgcgag cgtgatgtcc tcggcccgct gcacgtcgcg gtcgacccac acgccgaccg
      241 ggcgctcgcc ctcgaacagc cgctcgtacg agacgccgaa gcgacgggcg atgtccttct
      301 gcagcggcgt gatgccgatg ggccacgcat cgagggcctg ggcgctcggc tgctggccgt
      361 ggtcggccgt gaacaccatc acccactgct cctcgccgac cttgcggtcg agccacttcg
      421 tcaggtcctc gagggccgcg tcggagtact cgaggatctc acgcatctgc ggctcgacca
      481 tgttgtagtc gtggccgacg tcgtcgatct gcttgaagtt cgtgaagaag aggtccggga
      541 cgcggttcgc gccgaagtcc tcgcgcgcca aaagctcctt gatcaggcgc gtctggtaca
      601 gcgtccacac cgggctgtgg cggaccgtcg tcgggtcggt caggtcggcg tggcgcatcc
      661 acttcccgtc gagcttgccg tcctcgacgt cgaccgaccg gacgtcctcc tcccagccgg
      721 gcacgtcgtc gaggtagtac gggagcttgt agaggtcggg gttcgtgatc aagcggcccg
      781 gcccggtcac gcggtcgcca gcctcgcgcg cggcgatcgc cacgacgtcc ctgtcggcac
      841 ccggcagcat cgcaccgtgt cccatcatcc cgaggtggaa gtagcgatag gtgagcgcgc
      901 cgaccttcgg ccggttgccg ttgcggcggt cgaagaggtc accgaccgtc gtgagctcga
      961 gctcgtgcgg gggctggccg ttgagcgccc ccttgacctc gcccttcgcg ttgcgcatgt
     1021 agatgtcgac gacgccgtga cggcgcggga actcgccggt ccccatcgtg gagtggatcg
     1081 ccggcgtcac cgaaggcgac gacccgacca tcgcgcccgc gaccgacgtg ccgccctcga
     1141 tcaacgacgc gaggtgcggc cacgcacgcg gccaccggtt cagcacgttc catccgccgc
     1201 cgtcccacac gatctggacg atcagcttgg gcgaacgcgg gcgctgcgcg tcggggagca
     1261 gcgcctcctt gatcgcccgt cccgcgcgcc ccttctccca tcccatgcgc agcaggcgcg
     1321 ccatcgtcgg cgcgaggtcg gcgagcgtga tcttgcgcct caggtcgagg gtcccctgcg
     1381 cttcgacgaa ccccggcccg tagaagatca gcgggacctc ctgcacgtac tcccacggcc
     1441 ccgagtgcgt ggtgctgaag aagcggccga agaagtgcgg ctggcgcggg accaggaaca
     1501 cgtcgggcga acgtccccgg taatagccgc gcttgatcct ctgcagatgc tccagcggca
     1561 aggagcatgc ggactcgagc caggcgggcc ggtcgcgcag cgctatgggg ggctcggtcg
     1621 ccgtgggcgc gagggcggtc cccgaggtgc cgccggggaa gtcgccggtc gcctgcgacg
     1681 cggtcgagca cgccgccgcg acgacgcaca cgacggccac gcgccggcgc caccccctca
     1741 cggacacgtg agtcctgcgc atgccctgtc cgtttcgagc tgcgtctcga gctgcttcag
     1801 cacgaagggg ctcgggtcgt tgtccgggtt cgcgtcgccg aggaggttgc tcgtctgcca
     1861 cgggtcggcg gcgaggtcgt agaactcgcg gtaacgggtt cgcccgtcct tgaagtagta
     1921 ctggatgtac tggtagtcgt gcgtccggat cgacgcccac gtggggcggt tcgcagacca
     1981 gctcccccac tgctccgtca gcatccgcga gcgttcgagg gggccgaaca gcgagcgtcc
     2041 gtcgacctcg gtctgcacct ccggcagaag ccccgcgacg tcgaggacgg acggcgcgat
     2101 gtcgatgttg ccgacgagac ggtcgtcgac cccgcctgcc tcgacgcggc ccggccaccg
     2161 cacgaagaac gggacctgga tcgactcggt gtacgggagc ctcttcgcgt agaggccgtg
     2221 ctcgccgagc atcatcccgt tgtcggagat gaagaacgcg agcgtctcct ggtcctcgtc
     2281 gagctcttcg agcttgtcga acagcgagcc gaccatgtcg tccgccgaca tcagagtgcg
     2341 cagctgcttg tggtagatcg cctcgccctg ctccgccgag aacccgacgt tgtggacgaa
     2401 gcccggcttg tcgccgagcc tgtcctcggt gatcgccggg ttgtcgggcc acgacggcac
     2461 cggcgcctcc gcgtactcgt ccgacgagaa gaacgggaag tggggcgcgc tgacggtcac
     2521 gtacatgagc cagggggccg cgtcgtcctc ggcgtcgaac tcctcgagga actccatgct
     2581 ctgcttgccg atgtagtcgg tcgcgtactc ctggacctcg accatctcgc cgttcacgtt
     2641 gaactcgttg ttgtagtacc cggcccggaa cagcgcccac cggtcgaaga acctcgggct
     2701 ctgggtcacg tcccacgagt tgaggaaccg ccccacgagc gcggtcttgt agccggcgcg
     2761 ctcgtggagg tagcgctgca tcgtcaccat ctggttcatc ttcggcccgt cctggttgcg
     2821 ccggacgccg tggttgtggg cgtagcgccc ggtcatgatc gaggcgcgcg acgggcagca
     2881 ctggggcgtg gaggcgaagg cgtgggtgaa ctgggtcccg ccgcgcccga agatctcgag
     2941 cgtctccggc atgaccagca tctggtcgcg ctcgcgctcg tcgtccgtga cgatgacgag
     3001 cacgttcggc cggtcgtcct gcgacgacgc cggctccgct acgtagaccc ccgcgaccac
     3061 gacgcccacc cagcacaggg ccaggagggc gagtctcact cgctgcaccg ccacatcatc
     3121 ccttctttac ccgcccctcg aagccgtcgg ccacgtggac cacttctccg ccacagacga
     3181 ccgccgcgac cgggttccgg tcgggcctgt aggcgaggtg gtccaggctc gggacgtcca
     3241 ggacgaccgc gtcggcccgg tagcccggag caaggcgacc gacttcgtct tcgaggccga
     3301 gcgcggccgc cgcgttcacg gtcgcggcgg cgagggcctc ggcggggcgg aacccgtaca
     3361 cccggcacgc gagcgcgatc acgaacggca tcgaccgcac cggcgaggtc ccggggttga
     3421 ggtcgctgcc gagcgccacg atcgccccgc gtgacaggag gtcgcgcgcc ggcggcttct
     3481 tcgactgcct cagcgtgaac gtcgcccccg gcagcaagac cgcgacggtc tcggattgcg
     3541 cgaggtcccc gaccgcgtcg ggggccgagt ggttgaggtg gtcggcggtg tcgaaccccc
     3601 agcgcgctgc gaacgctgcc gtctgaccgt cggagagctg gtctgcgtgg acccgcatcc
     3661 gcatcccgag gccccgggcc gtctcgtgga ggcgggccgc gtgctcgagg gcgaacgcga
     3721 tcgtctcgac gtacaggtcg catgcggtcg cgaggccccc ggccgccgcc tcgggcagga
     3781 gctggccggc ggcgatcccg atccactcgc cctcggaccg ccccttcggg acggcatggg
     3841 cggcgaggca ggtcgtgacg accttctggg ggacccggcc ggcaagcgcc cgggcgagcc
     3901 cgagctggcg gagctcggcc tcgaccgaca ggccgtagcc ggacttcgtc tcgaacgtcg
     3961 tcgtaccgga ggcgagcatc tcccccgcga gctcgcccga gaagcccagc acctcgtcgt
     4021 cggaggcctc ccccaggagc cgcgccgagc ggaagatccc gccctcgcgc ccgtggagcg
     4081 tctcgtacct gacgcccgac agccgggccg cgtcctcgtc ggccctccag ccgaagaacg
     4141 ggaggtgcgt gtggcagtcg acgaagcccg gcacgacgac gcagcccgag gcgtcgaccg
     4201 tgacctcggc ggccgtctca ccgccggcga agcggccgtc tcgcgcggag aggacgccgt
     4261 cctcggtcac gtcgggggcc gccagggcct cggcccgcac gggcccggtc ccggcccagg
     4321 tgacgtgcgt ccccccggcg atcgtcagcg aagccgtcaa tcgtcgacct cgacgacgcg
     4381 gcggcgcgag accttcttgt agaggaacgt cccgtcgtcc gcctcgacgc gttcgacctc
     4441 gcccctctgc cggaggtacg cgaggtgggc gagggcctcg ccgagggcga gccggcgctc
     4501 gaagtggagc agctgggacc cgaagacctc gtcgacgatc gtggtggcgc tccggggctc
     4561 gcgcctgatg acctggagga tgttgccgag ccgccggtcg tggtggcgcg cgattacgcg
     4621 ggcgcgttcg gccccctcga ggaacggccg gccgtgcccc ggcagcacca tcttcggatc
     4681 caggtcctcg accttctcga gcgactcgag gaactcgccg agggggtcct cctcgccgcc
     4741 gcgctcgaag tcgatgtgcg gggtgatcgt cggcagcagg tggtcgcccg agatcagcag
     4801 gccgtcactc gcgttccaca ggcacacgtg cgaccgggca tgtcccggcg tgtggacgac
     4861 ggcc
//
LOCUS       DASQDO010000356         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_99485, whole genome shotgun sequence.
ACCESSION   DASQDO010000356 DASQDO010000000
VERSION     DASQDO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99485"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VEV43_10000"
     CDS             <1..520
                     /locus_tag="VEV43_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYP23896.1"
                     /translation="MFEALRAVPQPVIASVFGFALGGGFELVLSCDLVVAAEGTKLGL
                     PEARVGLLPAGGGTQLLTRKAGLARAKELIYTGRRFDAAEAHAWGLVQRVVPRAELGY
                     QTQDLALDVCRSSPVAVRAAKAATDGALGAPIEDGIEIEHRAWETVIASEDRAEGIAA
                     FNEKRDPQWRNR"
     gene            517..1422
                     /locus_tag="VEV43_10005"
     CDS             517..1422
                     /locus_tag="VEV43_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYP23897.1"
                     /translation="MSVRIREVGPRDGIQSEKADVPTADKVALIDALSETGLRCIEAV
                     SFVSPKAIPQMADAREVWGGITRREGVVYSALVPNRKGAEIAAEVGVDSMQVFIAASE
                     SYNLKNVRKPVRDSIEDVQAVVDVARAAGIPVEGTISTAFGDPYEGDVPPERVVEVSR
                     WMADTGIDTISYGDTTGMATPRRVADVVSALREELPGLTLNMHFHDTRGTGLANVLTA
                     LDLGIDYFDASVGGMGGSPYAAGATGNVATEDLIHMLADMEIETGVDLDKLLDAARIA
                     QGFIQGELPGKVLKAGPRWAVAGRA"
     gene            1422..>1738
                     /locus_tag="VEV43_10010"
     CDS             1422..>1738
                     /locus_tag="VEV43_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYP23898.1"
                     /translation="MFRRILIANRGEIAVRIARTCSALGVEAVAVHSDVDAQARHVAA
                     ATRAVHLPGVAPTETYLNVEAILEAARSTGAEAIHPGYGFLSESASFADAVVGAGVVW
                     VGPP"
BASE COUNT          264 a          587 c          641 g          246 t
ORIGIN      
        1 catgttcgag gccctgcgtg cagtgccgca accggtgatc gcgtcggtct tcgggttcgc
       61 gctgggcgga ggcttcgagc tcgtgctgag ttgtgatctc gtcgtcgcgg cggaggggac
      121 gaagctcggg ctgcccgagg cacgtgtggg tctcctgcct gccggcgggg ggacccagtt
      181 gctcacgcgc aaggccgggc tcgctcgcgc gaaggagctg atctacacgg gccgccgctt
      241 cgatgccgcc gaggcgcacg cgtgggggct cgtgcagcgc gtcgtcccgc gcgcggagct
      301 cggataccag acccaggacc tggcgctgga cgtctgccgt tcgtccccgg tcgcggtgag
      361 agcggcgaag gcggcgaccg acggcgctct cggagcgccc atcgaagacg ggatcgagat
      421 cgagcaccgg gcgtgggaga ccgtgatcgc gagcgaggac cgggccgagg gcatcgcggc
      481 gttcaacgag aagcgggacc cgcagtggcg caaccggtga gcgtgcgcat ccgagaggtc
      541 ggcccccgcg acggcatcca gagcgagaag gccgacgtcc cgacggccga caaggtcgcg
      601 ctgatcgacg cgctgagcga gacgggtctc cgctgcatcg aagcggtctc gttcgtgtcg
      661 cccaaggcca tcccgcagat ggccgacgcg cgcgaggtgt ggggcgggat cacgcggcgc
      721 gagggcgtcg tctactccgc tctcgtcccc aaccgcaagg gggcggagat cgccgcggaa
      781 gtcggcgtcg attcgatgca ggtcttcatc gccgcgagcg agtcttacaa cctgaagaac
      841 gtgcgcaagc cggtgcgcga ctcgatcgaa gacgtgcaag ccgtcgtgga cgtcgcacga
      901 gcggcaggga tacccgtcga gggaacgatc tcgactgcgt tcggcgaccc ctacgagggc
      961 gacgtcccgc ccgagcgtgt cgtcgaggtc tcgcgctgga tggcggacac cggcatcgac
     1021 acgatctcgt acggcgacac gaccggcatg gcgacgcctc gacgcgtcgc cgacgtcgtg
     1081 agcgctctgc gcgaggagct gcccggcctc acgctcaaca tgcacttcca cgacacgagg
     1141 ggcacggggc tcgccaacgt gctcaccgct ctcgacctcg gcatcgacta cttcgacgcg
     1201 tcggtcggcg gcatgggcgg cagcccctac gcggccggcg ccaccggcaa cgtcgcgacc
     1261 gaggacctga tccatatgct tgcggacatg gagatcgaga ccggcgtcga cctcgacaag
     1321 ctgctcgacg cggcccgcat cgcccagggg ttcatccagg gcgagctgcc gggcaaggtc
     1381 ctgaaggccg ggccgcgctg ggcagtcgca gggcgggcct gatgttccgg cggatcctga
     1441 tcgcgaaccg cggcgagatc gcggtccgga tcgcgcggac gtgcagcgcg ctcggtgtcg
     1501 aagctgtcgc ggtgcactcc gacgtcgacg cgcaagcgcg ccacgtcgcc gcggcgaccc
     1561 gagcggtcca cctcccgggc gttgccccta ccgagaccta cttgaacgtc gaggcgatcc
     1621 tcgaagcggc tcgctccacg ggggccgagg cgatccaccc gggctacggc ttcctgtcgg
     1681 agagcgcctc gttcgcggac gccgtggtcg gcgcaggggt cgtctgggtc ggtccgcc
//
LOCUS       DASQDO010000357         7787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_894828, whole genome shotgun sequence.
ACCESSION   DASQDO010000357 DASQDO010000000
VERSION     DASQDO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894828"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..505
                     /locus_tag="VEV43_10015"
     CDS             65..505
                     /locus_tag="VEV43_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23899.1"
                     /translation="MRDAPEPKSIEVFLAEVHDQLAAQRSHVESLDAKAGVVLGFSGL
                     LAAVAPDGNALWMDLARGAAIFAGIFALFGLLPRGYAVVDLRAFQRDFLTAQPTTAQL
                     VLFDTQLNVLADTAGVIDHKSRRLRASIAALLVAIIFAYVGPVT"
     gene            736..963
                     /locus_tag="VEV43_10020"
     CDS             736..963
                     /locus_tag="VEV43_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP23900.1"
                     /translation="MDETGRQPTSSATSASLMGGFLTVAEVARQLRVSNMTVYRLVKA
                     GDLAAVRVGRGYRIRTEDVRKYLQQRYMDAG"
     gene            1039..1935
                     /locus_tag="VEV43_10025"
     CDS             1039..1935
                     /locus_tag="VEV43_10025"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HYP23901.1"
                     /translation="MRATLDETYEKLLSSPGVVFLLGGIDTGKTTFGIELVRRAVAAG
                     IPSAIVDADIVQSTVGPPTTAGLKVCRTEEDLTRDSLRKADSLGFVGSLIPKGHLLPL
                     VTNAAKLVNRAKEAGAKLVVVDTTSLVSGIYGQSLKFFKMDLIAPDFTVAFERGGELE
                     PLVGIAQRFVPGEVVEIEVSPDARIRTVDERIAFREEQFASYFAVGASRWRVKPTVFM
                     PTLPPEFDLALLDGLVVGMEDGRGSCVGIGVLEHDASEDLLRMVSPVTEGVRGLRLGS
                     QRIDTSGQSRGPVDLRQLFGSE"
     gene            1963..2064
                     /locus_tag="VEV43_10030"
     CDS             1963..2064
                     /locus_tag="VEV43_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HYP23902.1"
                     /translation="MPSLIKKRRKKMRKKKHRKMLKKTRWQRRQQGK"
     gene            complement(2143..2421)
                     /locus_tag="VEV43_10035"
     CDS             complement(2143..2421)
                     /locus_tag="VEV43_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYP23903.1"
                     /translation="MTPLQIWQDQAACKTLPLEVFFPPAEQEAEAAKAICSGCTVREP
                     CLEAALAAGERFGIWGGMSSDERQTVAARRRARTASARAAGLDVGLGV"
     gene            2517..3335
                     /locus_tag="VEV43_10040"
     CDS             2517..3335
                     /locus_tag="VEV43_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYP23904.1"
                     /translation="MKKRETLTFLSDYGLEDEFVGVCQGVILRIAPHVKIIHIHHNIL
                     RQSIRHGAIVLEQSIRFMPESVHLAVVDPSVGSERRAIVIESGWGEIFVGPDNGLLMP
                     AAEQCGGVKRAFEILDRSFLLTPISRTFQGRDVFSPAAAHIANGVDPSDLGPAIPVDD
                     LVRLEIPEAWVHDDHLHAEVLQVDRFGNLQLNVNRDQLETVHLVNGAKVEVRMEGHRI
                     HVPLASTFADVEDGEFVLVEDSYRHLSLAVNKGDAAARLRAHAGSTAIVGPTGG"
     gene            3366..4415
                     /locus_tag="VEV43_10045"
     CDS             3366..4415
                     /locus_tag="VEV43_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP23905.1"
                     /translation="MREVPQGKRILVTGVSRFLGLRLAKRLEEQDGVAAIVGVDLEEP
                     PVPVRGLEFIRADISSPLIARVIEATKIDTIVHTNIGSNPARLGGRSRMKENNVIGTM
                     QLLAAAQRSDRVKKVVMKSSTAVYGSDPAEPSLIPEGHAQRQVDLAGYGKDCAEAETY
                     ARDFGRRRPDVDLVILRTQNVIGPTVRTSMTDYLSLPVIPTALGFDPRLQLLHEEDAV
                     EALAVGALGDSRGIFNVAADGVVYLSKAIRLLDRVELPLLLPMAQTTAGLLRRFGLVD
                     FPTDQLKLIVYGRVVSTQRAKEALGFRPKRTTEETLMDFRDHRRADLVPSAEKHPAWE
                     RELFDYLRSRQPEKV"
     gene            4415..5482
                     /locus_tag="VEV43_10050"
     CDS             4415..5482
                     /locus_tag="VEV43_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYP23906.1"
                     /translation="MAEAEIIRLHDHRTKPDADGEAERKRCAATTATGRPCRNFALPD
                     AEYCSIHARGSTAAQRRHPAQGIETPSRPPEPEPTRTVLDGIGDFLRRRLDGAYPVDD
                     FGYDPELHREVLLPLQRALYERYFRVRTLGVDRIPHEGAALLVGNHSGTIPVDAVMVQ
                     HAVATEHPRHRTVRNVAADLAFNMPFVGHLARKTGNAVACDEDAHELLRRGEVVGVYP
                     EGYKGVGKGWKDRYKLQRFGRGGFIEVALRTRTPIVPVAIVGAEEAFPMIANAKLLAR
                     TFGFPYFPITPTFPLLGPLGLLPLPSRWLIEFGEPIPMDDYPEDAAEDAMLVFDLTDR
                     IRDTIQQMLYRNLSLRGRAFI"
     gene            5644..6156
                     /locus_tag="VEV43_10055"
     CDS             5644..6156
                     /locus_tag="VEV43_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23907.1"
                     /translation="MSEDAAGQAGRVGGLESLYQRHASDAVRLAYLLTGDASLAQDVA
                     HEAFVRVGRKLFGLRNDEHAKAYLYRTTVNLCRGRGRRQKSERAAVQRLQGAPPVQPP
                     DLAARDELWTALLTLPVRQRSALFFRYYEDLSEAGAAEALGCSVSALKALVNRGLKEL
                     RSQLEGARDD"
     gene            6149..7309
                     /locus_tag="VEV43_10060"
     CDS             6149..7309
                     /locus_tag="VEV43_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23908.1"
                     /translation="MTDVEARLRAALHDTLDPVGVDPARARPAIRAARRGRALRVVTA
                     VVATSVVAAAAVSTPWRDGRPRPAADSADVGAFVASFGRHGQATVVVDVVKSQLCVDL
                     TRVRARYGAAVRYDVQGPDDPRVGSFDSTIRARGAPACTTVDEAAARRLVREPRLHYL
                     EVDPGESGRASALAPLDVEPQYAPDRLEVVCSQTGAVALAPEVQPRLDGVHLRIHDQD
                     GAWDQVTIRPAGGGTIGMRLTPVGPLENATSLAPGTYSIVCFRDGAEPGFEPPDATRY
                     ATFSVVDPNDLWNDAELACDATRQLREIVTDEPIGIPSVPPDFDLLARDHVPGLRPGD
                     ELERPAYPESATRREARTVARDGVRIATLYFSRGHGRWSILPVVCDGSGIGG"
     gene            complement(7314..7724)
                     /locus_tag="VEV43_10065"
     CDS             complement(7314..7724)
                     /locus_tag="VEV43_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP23909.1"
                     /translation="MTARVLVVDDTEHVRSMLVDMLELDGFDVVGQAASGAEALGAAA
                     AYDPDVIVMDYKMPGMDGLSSARAIRATRPNQGIILYTAYLDQKLETEARDAGVALCV
                     GKVEGLSQLERHITELCRDFGDTGGHPKPGASRA"
BASE COUNT         1217 a         2793 c         2638 g         1139 t
ORIGIN      
        1 taggggatcg ttaggttggg ctggacaacc gagcccgcgc acgagccgaa gggaaagcct
       61 ttccgtgcgc gacgcgcccg agccgaagtc gatcgaggtg ttcctcgccg aggtccacga
      121 tcaactcgcc gcgcagcgaa gccacgtcga aagcctcgac gccaaggcag gcgtcgtact
      181 tggtttctcc ggcctgctcg cggcagtcgc gcctgacggg aacgcgctgt ggatggacct
      241 agcgcgcggc gccgccatct tcgccggcat cttcgcgctc ttcggtttgc tgccccgcgg
      301 ctacgcggtc gtcgaccttc gggcgttcca acgtgacttc ttgaccgcgc aaccgacgac
      361 ggcgcagctc gtcctcttcg acacgcagtt gaacgtcctc gccgatacgg cgggtgtgat
      421 tgatcacaag tcacgccggc tgagggcttc catcgcggcg cttctggtcg ccatcatctt
      481 tgcgtacgtc ggaccggtca catgacgacg agaaaggaag ggcgatgaag gacgagctgt
      541 cgacagcgaa cgatcgcgaa gacgacgccc gcgggattcc gtctcacgcg caccctgagt
      601 ggatcatgtc cctggacggc caccgcttga tcgatcgtcc gcggaatctc cgcgagtggt
      661 taaaacacat cttccgccgg ccccgaaggc ccacctaagc gggcaaaaag ccgtcggtat
      721 atgcttcccg ccgtcatgga cgagaccggg cggcagccga cctccagcgc aaccagcgct
      781 tccctcatgg gcggcttcct caccgtcgcc gaggtcgcca gacagctccg cgtctccaac
      841 atgaccgtct accggctcgt caaagcgggc gacctcgccg ccgtgcgcgt cggccgcggc
      901 tatcgcatcc gcaccgagga cgtccgcaag tacctgcagc agcgatatat ggatgcaggc
      961 taggcagccg gccctcggcg ccaccaagac cgtcctgcgg gacggtcttt ttgttgaccc
     1021 ctgcgcggga ggacgtcgat gagggcgacg ctcgacgaga cctacgagaa gctcctttcg
     1081 agccccggcg tcgtcttcct cctcggcggc atcgacaccg gtaagaccac cttcggcatc
     1141 gaactcgttc gccgggccgt cgccgccggc atccccagcg cgatcgtcga cgccgacatc
     1201 gtccagtcga cggtcggccc ccccaccacc gccggcctca aggtctgcag gaccgaggag
     1261 gacctcaccc gcgacagcct ccgcaaggcc gacagcctcg gcttcgtcgg gtccctgatc
     1321 cccaagggcc acctgctccc gctcgtcacc aacgccgcca agctcgtcaa ccgggcgaaa
     1381 gaggccggcg cgaagctcgt cgtcgtcgac accaccagcc tcgtgtccgg catctacggc
     1441 cagagcctca agttcttcaa gatggacctg atcgcccctg acttcaccgt cgcgttcgag
     1501 cggggcggcg agctcgagcc cctcgtcggc atcgcccaga ggttcgtccc cggcgaggtc
     1561 gtcgagatcg aggtctcgcc cgacgcccgc atcaggacgg tcgacgagcg gatcgccttc
     1621 cgcgaggagc agttcgcgtc ctacttcgcg gtcggggcct cccgctggag ggtcaagccg
     1681 acggtcttca tgccgacgct gccccccgag ttcgacctgg ccctgttgga cgggttggtc
     1741 gtgggcatgg aagatgggcg aggatcgtgc gtcggcatag gggtcctcga gcacgatgca
     1801 agcgaggacc tgctgcgcat ggtgtcgccc gtgaccgaag gcgtccgggg cctccggctc
     1861 ggctcccagc ggatcgacac ctccgggcag tcacgcgggc cggtggacct gaggcagctg
     1921 ttcgggtccg agtaggacca cgaaagagga aggaaagtcg caatgccgtc tttgatcaag
     1981 aagcgccgca agaagatgcg caagaagaag caccgcaaga tgctcaagaa gactcgctgg
     2041 cagcgccgac agcagggcaa gtaagggaag cgggccgggc ctgtgagctc ggcccgtttg
     2101 ttctgcggtc ccgccgaggc ggccccgccc gcggccgggc tgctagaccc ccagccccac
     2161 gtccaggccc gcggccctag cgctcgcggt cctcgcccgg cgccgggccg cgacggtctg
     2221 gcgttcgtcg ctcgacatcc cgccccagat cccgaagcgc tcgcccgcgg ccagggcggc
     2281 ttcgaggcac ggctcgcgca ccgtgcaccc cgagcagatc gccttcgcgg cctccgcttc
     2341 ctgctcggcc ggagggaaga acacctctag cggaagggtc ttgcacgcgg cctggtcctg
     2401 ccagatctga aggggagtca cgcgcggagt gtaagaacgc cgcggagcgg gagtcaatgg
     2461 gcttggtttc tttccgcgct gcccggggtc gcgggccggt cgttaggatg ccccgcgtga
     2521 agaagcggga gacgctgacg ttcctgtcgg actacggcct ggaggacgag ttcgtcggcg
     2581 tatgccaggg cgtgatcctc cggatcgccc cgcacgtcaa gatcatccac atccaccaca
     2641 acatccttcg gcagagcatc cgccacggcg cgatcgtgct cgagcagtcg atccgcttca
     2701 tgcccgagtc ggtccacctc gcggtcgtcg acccgagcgt cggctccgag cgccgggcga
     2761 tcgtcatcga gtcgggctgg ggcgagatct tcgtcggccc ggacaacggc cttttgatgc
     2821 cggcggcgga gcaatgcggc ggcgtcaagc gagcgttcga gatcctcgac cggagcttcc
     2881 tgctgacgcc gatctcgcgc acgttccagg gccgcgacgt cttctcgccc gcagccgctc
     2941 acatcgcgaa cggcgtcgac ccctcggacc tcggcccggc gatccccgtc gacgacctcg
     3001 tccgtctcga gatccccgag gcgtgggtcc acgacgacca cctccacgcc gaggtgctgc
     3061 aggtcgaccg cttcgggaac ctccagctca acgtgaaccg cgaccagctc gagacggtcc
     3121 acctcgtgaa cggcgcgaag gtcgaggtgc ggatggaggg ccaccgcatc cacgtcccgc
     3181 tggcgagcac gttcgccgac gtcgaagacg gcgagttcgt gctggtcgag gactcgtacc
     3241 gccatctatc gctggcggtg aacaaaggcg acgcggcagc acgcctacgc gcccacgcgg
     3301 gctcgaccgc gatcgtgggc ccgaccggcg gatagcccgg agggaccggc gggtactctg
     3361 cttccatgag ggaggtcccg caaggcaagc gcatcctcgt caccggtgtg tcgaggttcc
     3421 tcggcctccg gctggccaag cgactcgaag agcaggacgg cgtcgccgcg atcgtcggcg
     3481 tcgacctcga agagccgccg gtgccggtgc gggggctcga gttcatccgc gccgacatct
     3541 ccagccccct catcgcgcgc gtgatcgagg ccacgaagat cgacacgatc gtccacacga
     3601 acatcggctc gaacccggcg aggctcggcg gccgctcccg catgaaggag aacaacgtca
     3661 tcgggacgat gcagctcctc gccgcggcgc agcgctccga ccgcgtgaag aaggtcgtga
     3721 tgaagtcgtc gaccgccgtg tacggatcgg accccgcgga gccctcgctg atcccggagg
     3781 ggcacgccca gcgacaggta gacctcgccg gctacggcaa ggactgcgcc gaggccgaga
     3841 cgtacgcacg cgacttcggc cgtcgccggc ccgacgtcga cctcgtgatc cttcgcaccc
     3901 agaacgtgat cgggccgacg gtgagaacga gcatgaccga ctacctctcg ctcccggtga
     3961 tcccgaccgc gctcggtttc gacccgcgcc tccagctact ccacgaggag gacgcggtgg
     4021 aagcgctcgc ggtcggcgcg ctcggagact cccgcgggat cttcaacgtc gcggccgacg
     4081 gcgtcgtgta cctgtcgaag gcgatacgcc tgctggaccg cgtcgagctc ccgctgctgc
     4141 tcccgatggc ccagacgacg gcgggcctcc tgcggcggtt cgggctggtc gacttcccga
     4201 cggaccagtt gaagctcatc gtctacggcc gcgtcgtcag cacccagcgt gcgaaggagg
     4261 cgctggggtt ccggcccaag cgcacgacgg aggagacgct catggacttc cgcgaccacc
     4321 gccgtgcgga cctcgtgccc tcggcggaga agcaccccgc gtgggagcgc gagctgttcg
     4381 actacctcag atcccgacag ccggagaagg tgtgatggcg gaagcagaga tcatcaggct
     4441 gcacgaccac cggacgaagc ccgacgccga cggcgaggcc gagcgcaagc gctgcgcggc
     4501 gacgaccgcg accgggcgcc cgtgccgcaa cttcgccttg cccgacgccg agtactgcag
     4561 catccacgcg cgcggcagca ccgccgcgca gagacggcac cccgcgcagg ggatcgagac
     4621 gccgtcgcgg ccccccgagc ccgagcccac tcgaaccgtc ctcgacggca tcggcgactt
     4681 cctccgcagg aggctcgacg gcgcgtaccc ggtcgacgac ttcggctacg accccgagct
     4741 ccatcgcgag gtgctgctgc cgctgcaacg cgcgctctac gagcgctact tcagggtcag
     4801 gacgctcggt gtcgaccgga tcccgcacga gggggccgcg ctgctcgtcg gtaaccactc
     4861 gggcacgatc ccggtcgacg cggtgatggt gcagcacgcg gtcgctaccg agcacccgcg
     4921 ccaccgcacc gtccgcaacg tcgcggcgga cctcgcgttc aacatgccgt tcgtcggcca
     4981 cctcgcgcgc aagaccggca acgcggtcgc gtgcgacgag gacgcgcacg agctgctgcg
     5041 gcgcggcgag gtcgtcggcg tctaccccga gggctacaag ggcgtcggca agggatggaa
     5101 ggaccgctac aagctccagc gcttcggccg cggcggcttc atcgaggtcg cgctgcggac
     5161 gcgcacgccg atcgtcccgg tcgcgatcgt gggggccgag gaggcgttcc cgatgatcgc
     5221 caacgcgaag ctgctggccc gcacgttcgg cttcccgtac ttcccgatca cgccgacgtt
     5281 cccgctgctc ggccccctcg ggctcctgcc gctgccgtcg cggtggctga tcgagttcgg
     5341 cgagccgatc ccgatggacg actatcccga ggacgcggcc gaggacgcca tgctggtgtt
     5401 cgacctcacg gaccggatca gggacacgat ccagcaaatg ctctaccgca acctgtcgct
     5461 gcgaggccgg gcctttatct agcccccata aatgcgcatc acgccgagga cgcccgcgac
     5521 cgtgatcgcc agcagcaaca ggacgatggc gtagagggtg ttccttctca cgaccgaatt
     5581 ctaggcgccg gcgcgcaacc tcgagccccc ctgacgcgtg tatatggcgt gtacgtggtg
     5641 ggggtgtccg aagacgcggc gggccaggcg ggtcgtgtcg gcgggctgga atccctctac
     5701 cagcgtcacg cgtccgatgc ggtgaggctc gcgtacctgt tgacgggcga cgcgagcctc
     5761 gcccaggacg tcgcccacga ggcgttcgtc cgcgtcggcc gcaagctctt cggcctccgc
     5821 aacgacgagc acgccaaggc ctacctctac cggacgacgg tcaacctgtg ccgtggacgc
     5881 ggccgacggc agaagagcga acgggccgcg gtgcagcgcc tccagggagc gccgccggtg
     5941 cagccgcccg acctcgcggc ccgcgacgag ctctggacgg cgctgttgac gcttccggtc
     6001 cggcaacggt ccgcgctgtt cttccgctac tacgaggacc tgtccgaagc gggggccgcg
     6061 gaggcgctgg gctgctcggt gagcgcgttg aaggcattgg tcaaccgggg cctgaaggaa
     6121 ctgcgctcgc agctcgaagg agcgagagat gactgacgta gaggcgcgcc tacgagccgc
     6181 gctgcacgac acgctcgacc ccgtcggcgt cgaccccgcg cgcgctcgac cggcgatccg
     6241 cgccgcgcgc cgaggacggg cgctgagggt ggtgacggcg gtcgtggcga cgtccgtcgt
     6301 cgcggctgcc gcggtctcca ctccgtggcg tgacggccgg ccgcgaccgg cggcggactc
     6361 ggcggacgtc ggtgcgttcg tcgcttcgtt cggccgccac ggccaggcga cggtcgtcgt
     6421 cgacgtcgtg aagtcgcagc tctgcgtcga cctgacgcgc gtgcgggcac gttacggagc
     6481 ggcggtccgc tacgacgtgc agggcccgga cgatccccgc gtcggatcgt tcgactccac
     6541 gatccgggcc cgcggcgcgc cggcgtgcac gaccgtcgac gaggccgcag cgcgccgcct
     6601 cgtccgggag ccgcgcctcc actacctcga ggtcgacccg ggcgagagcg gacgtgcgtc
     6661 ggcgctcgcg ccgctcgacg tcgagcccca gtacgcgccg gaccgcctcg aggtcgtgtg
     6721 ctcgcagacg ggggccgtgg ccctggcccc cgaggtgcag ccgcggctcg acggggtgca
     6781 cctgcggatc cacgaccagg acggcgcgtg ggaccaggtc acgatcaggc cggccggcgg
     6841 gggcacgatc ggcatgaggc tcacgcccgt cggcccgctg gagaacgcca cgagcctcgc
     6901 ccccgggacg tactccatcg tttgcttccg cgacggcgcc gagcccggct tcgagccgcc
     6961 ggacgcgacg cgctacgcga cgttctcggt cgtcgacccg aacgacctgt ggaacgacgc
     7021 ggagctcgcc tgcgacgcga cgcgccagct ccgcgagatc gtcaccgacg agcccatcgg
     7081 gattccgtcc gtcccgccgg acttcgacct gctcgcccgc gaccacgtgc cgggcctgcg
     7141 tccgggcgac gagctagagc gcccggccta tccggagagc gcgacccggc gagaggcacg
     7201 cacggtagcg cgcgacggcg tcaggatcgc cacgttgtac ttcagccggg gccacgggcg
     7261 gtggtcgatc ctgccggtcg tgtgcgacgg aagcgggatc ggcggctagg ccgctaggcc
     7321 cggctcgctc ccggcttcgg gtgtccgccc gtgtccccga agtcgcggca cagctcggtg
     7381 atgtgccgct ccagctggct cagcccctcg accttgccca cgcacagcgc tacgccggcg
     7441 tcgcgggcct ccgtctcgag cttctggtcg aggtaggcgg tgtagaggat gatcccctgg
     7501 ttcggccggg tcgccctgat cgcccgggcc gacgagagcc cgtccatccc gggcatcttg
     7561 tagtccatca cgatgacgtc ggggtcgtat gccgcggcgg cgccgagggc ctcggccccc
     7621 gaggcggcct gcccgacgac gtcgaacccg tcgagctcga gcatgtccac gagcatcgac
     7681 cggacgtgct cggtgtcgtc cacgacgagc acccttgcgg tcacaggcgt ccctcccagt
     7741 gggtccgtcc tccggccaca gccgggaaac ggctcaacgc gtctgcc
//
LOCUS       DASQDO010000358         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_343947, whole genome shotgun sequence.
ACCESSION   DASQDO010000358 DASQDO010000000
VERSION     DASQDO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343947"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VEV43_10070"
     CDS             complement(<1..286)
                     /locus_tag="VEV43_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23910.1"
                     /translation="MTCLAAAAVVAALVPSPARAATIVCETLVESTPSVRADLDGDGY
                     PEYRVPSIQDVTLCSDAGASYVTNTPRTENCFVGWHPDCVAVYVTLMPAEA"
     gene            745..945
                     /locus_tag="VEV43_10075"
     CDS             745..945
                     /locus_tag="VEV43_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23911.1"
                     /translation="MRKFLAIVGVTAAVFVPMSSSFAYTVHESGSLHLGSCDVNYDSG
                     TYSYTPGGSFSATGPSASRTCS"
     gene            1054..1254
                     /locus_tag="VEV43_10080"
     CDS             1054..1254
                     /locus_tag="VEV43_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23912.1"
                     /translation="MRKALVVIGMAIGLVIPSSAALAYTVHESGSVGAGGCDLTYDSG
                     TYSWDPIYGPVSAQGPTAGYDC"
     gene            1354..2349
                     /locus_tag="VEV43_10085"
     CDS             1354..2349
                     /locus_tag="VEV43_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23913.1"
                     /translation="MRIRRLTLVVAAGCVALAPFASSTAAASDPAPRFEDAVTLPAPP
                     GTDFASAEASVPPSPAAGGCPTRIPRSNVDRPDLQRGPQVHVVYLVADDFGDERLDMD
                     GTIECSVRAWGQWFVEQTDGIRWRIDTFRGKGRKGPELVDVTFVRSTVPGAQLNSTAN
                     VEAELVKAGLVDQQKLYIAYVAADGAGTCGRGRYPLASTGDDGAFGGHGRFAYAYLFG
                     SDGCHGHEFGTPGNPRWLEATVMQEMLHSEGVVPLGAPHACTGIDPMPNHLCTPGLGL
                     TELAAVGLDPERIDVMFPFVSVPLVEKVLDRDRDDYFGHSLPLRDLSESPYLIQR"
     gene            2395..2682
                     /locus_tag="VEV43_10090"
     CDS             2395..2682
                     /locus_tag="VEV43_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23914.1"
                     /translation="MKTIRTLSALALVAGVLMIPAQAQADTVAVAPACEVHYDSGHAS
                     VNPSTGEVDFESPSAGATTSGCFTLHADLSAVWDLIPPLPCCIEDWISGDS"
     gene            2714..3022
                     /locus_tag="VEV43_10095"
     CDS             2714..3022
                     /locus_tag="VEV43_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23915.1"
                     /translation="MKTIRTLCAFALVAGVLMIPALAKADTVTVAPGCEVHYDSGHAS
                     VDPRTGAVDFESPSASASTGGCFTTHITIEDLLPSICCIIRDPLPPPCCIDDWLGISW
                     "
     gene            complement(3073..4983)
                     /locus_tag="VEV43_10100"
     CDS             complement(3073..4983)
                     /locus_tag="VEV43_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M6"
                     /protein_id="HYP23916.1"
                     /translation="MQTARRTTVALLSAALLIGGAAPAVARPTAQGPARKPKLPRPID
                     PQSWEVPEDMTWGDYRRIPGTDWKNPEYEAPKTLRAALILGDFQDQKFRVAETTVDPT
                     GQRGLGVEDPAAYWTNLLFKSTNPKNAQRGHTVGEYWLENSYGLIDVDAEGFGPYTME
                     GNMYEYGVTDFSGSEDCPDDSGCDGDFDTELVQASLADVTTSVQEHGQFDFRFLLHAG
                     YDESTVWLNYGQMLFAEPEDVTEKYGPPAFPDWRNWAGTRYVDWTSFFAAQQIWSHAL
                     PGALATEGESDGGSVYAHELSHVLGVLDNYNNPYAEDPIRSYSGPWDMMSRGTFNGPG
                     GPWERWTIPPTGGATMGAHHMIRNKIRLGFLPPAEVLTVNREALSAAPIRARILQRES
                     PPETADPTLFSGIRIVLGEDRSTCESSDPKCDRGGYDFYDVEVVNRQGFDSFLPDHGV
                     IIAKTKTADASPFIWVIDSHPKNMRKVDYVRADGEKFYYTVGDYRQLADAAFHAGTGR
                     KVVNHFVDEENELAFFVLAKEKDGGRLIYEVAVQSTAAPALAEASVAKRSGKVRKGRV
                     TKFEFEVTNDGAGPGIFRLKATRKGRVKVRLLNDLLWLDGGETKVVTVHALGRGGRAK
                     VGLKAAPAVPPA"
     gene            5079..>5558
                     /locus_tag="VEV43_10105"
     CDS             5079..>5558
                     /locus_tag="VEV43_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23917.1"
                     /translation="MDYSPAPEERARLGPVNPLTVAVAVVLLSAGGAFGWRMRTSAPS
                     ARPTPEAAAAALALERVDGEPMCGPRGRDDVRDEMLRGDSGQLPERLAEYLPKRSPLP
                     GYDETSSHAIDPASETEFVSGAVDGHVASFRPEGEGGFDVYAYRFLTRRAAADAVADN
                     "
BASE COUNT          795 a         1947 c         1895 g          921 t
ORIGIN      
        1 gggcctccgc cggcatcagc gtcacgtaga cggcgacgca gtccgggtgc cagccgacga
       61 agcagttctc ggtccgcggc gtgttcgtca cgtacgaggc tcccgcgtcc gagcacagcg
      121 tcacgtcctg gatgctcggc acccggtact cggggtagcc gtctccatcc aggtcggccc
      181 gcacgctggg cgtggactcg acgagcgtct cgcagacgat cgtggcggcc cgagccggag
      241 acggcacgag ggccgccacg acggccgcgg cggccaggca ggtcaaaagc ccccggcgac
      301 gccggggttc ggtgcatgac ggcgcgagtg cgtgtcgcgt cgttgagata cgcaatctcg
      361 ttcctttctg tgggtacgga gaggcaagga cggcgcgcgg ctggacccgg ctgggccccc
      421 caggtatccc acccctttct tgtcgtcggc ttgcccgcga gctcgctttg ccggtgacag
      481 cgttcgtcac aacgcggact agttcctcct gccgggtgaa gaaaaattct tcctgattga
      541 caggcagcgt ttagatttcc gccccctcga ccccctgaca ccgctgcttg ccttcgacaa
      601 attctttgat tgtggaggca ggttgcaagt caagctcggc gaatctgggg ccagcggcgg
      661 ttggtttgct tctccgcccg ctaggcaggc gaccggatcg ggcggaagcg cccggtccac
      721 gcacttgggg aagggggtga caacttgcgt aaattcctcg cgatcgtggg ggttacggcc
      781 gcggtcttcg tgccgatgag ctcgtccttc gcgtacacgg tccacgagtc cggctccctc
      841 cacctgggca gctgcgacgt caactacgac tcgggtacct actcgtacac gccgggtgga
      901 agcttctcgg ccaccgggcc ttcggcgagc cgcacctgct cgtagttcgg ccgtgcggcg
      961 cggcccgtcc gggcagtcgc cgcctctgaa cggaaaaaac ggaaacgcag ccttgcccga
     1021 tcgcgggcgg gctcatctcg aagggagctt caattgcgta aggcactcgt cgtcatcggc
     1081 atggccatcg gcctcgtcat cccctcctcg gcggctctcg cctacacggt ccacgagtcg
     1141 ggcagcgtag gcgccggcgg ttgtgacctg acctacgact ccggcacgta ctcctgggac
     1201 ccgatctacg gcccggtctc ggcccagggt ccgaccgccg gctacgactg ctagttccgt
     1261 tccatccgcc gggcggggtc tcacggcccc gcccggcgtt taaatccgct gtttccgcag
     1321 gagagtgagc agatgaagcg aaccgagcca ggtatgagaa tccgacggct gaccctcgtg
     1381 gtcgctgccg gctgtgtcgc cctggcgccg ttcgcctcgt cgaccgcggc ggcgtccgac
     1441 ccggccccca ggttcgagga cgcggtcacg ctgcccgcgc ccccgggtac cgacttcgcc
     1501 tcggccgagg cttcggtgcc cccctcgccg gctgccggcg gatgccccac gcgcatccct
     1561 cgctcgaacg tcgaccggcc cgacctccag cgggggccgc aggtccacgt cgtctatctg
     1621 gtggcggacg acttcgggga cgagcgcctc gacatggacg ggacgatcga gtgctcggtc
     1681 agggcctggg gccagtggtt cgtcgagcag accgacggca tccggtggcg catcgacacc
     1741 ttcaggggca aggggcgcaa gggccccgag ctcgtggacg tcaccttcgt ccgttcgacc
     1801 gtccccggcg ctcagctgaa cagcaccgcg aacgtcgagg ccgagctggt gaaggccggg
     1861 ctggtcgacc agcagaagct ctacatcgcc tacgtggcgg ccgacggcgc cgggacgtgc
     1921 ggacgggggc gctacccgct cgccagcacc ggtgacgacg gcgccttcgg gggtcacggc
     1981 cgcttcgcct acgcgtacct gttcgggtcg gacggctgcc acggacacga gttcggcacg
     2041 ccgggcaacc cgcgctggct cgaagcgacg gtgatgcagg agatgctcca cagcgagggc
     2101 gtcgtcccgc tgggtgcgcc ccacgcatgc accgggatcg acccgatgcc caaccacctc
     2161 tgcacgccgg ggctcggcct caccgagctc gcggccgtcg ggctcgaccc cgagcgcatc
     2221 gacgtgatgt tcccgttcgt gagcgtgccg ctcgtcgaga aggtcctcga ccgtgaccgg
     2281 gacgactact tcggtcactc cctgccgctg cgggacctga gcgagagccc gtacctgatc
     2341 cagcgttagc tgggtccttc acacgacccg aataaataga ttggagaaag tctcttgaag
     2401 acgatcagaa ccctttccgc gctcgcgctc gtcgcgggcg tcctgatgat cccggctcag
     2461 gcccaggccg acacggtcgc cgtcgcaccg gcctgcgagg tccactacga ctcgggacac
     2521 gcgtccgtga accccagcac cggcgaggtc gacttcgagt cgccgtcggc cggcgccacc
     2581 accagcgggt gcttcacgct ccacgccgac ctgagtgcgg tctgggacct gatcccgccg
     2641 ctgccgtgct gcatcgagga ctggatcagc ggcgactcgt aagcgtccgc tccgagacct
     2701 ggggagaact ttcttgaaga ccatcagaac cctttgcgcg ttcgcgctcg tcgcgggcgt
     2761 gctgatgatc ccggctctgg ccaaggccga caccgtcacg gtcgcgccgg gctgcgaggt
     2821 gcactacgac tcgggccacg cctcggtcga cccgcgtacc ggcgcggtgg acttcgagtc
     2881 gccgtcggcg agcgccagca cgggcgggtg cttcacgacc cacatcacga tcgaggacct
     2941 gcttccgtcg atctgctgca tcatccggga tccgctcccg ccaccgtgct gcatcgacga
     3001 ctggctcggc atcagctggt aggaccgtgc gagcgttcca ggggggcggc cacggccgcc
     3061 cccctgtcgc gttcaggcag gcgggactgc gggcgccgcc ttcagcccga ccttggcccg
     3121 gcccccgcgc cccagcgcgt ggacggtgac gaccttcgtc tcgccgccgt ccagccacag
     3181 caggtcgttc agcaggcgga ccttcactcg gcccttgcgc gtcgccttca gccggaagat
     3241 ccccggcccg gcgccgtcgt tcgttacctc gaactcgaac ttcgtgacgc ggcccttgcg
     3301 gaccttgccg gagcgcttcg cgaccgacgc ctccgctagg gcgggggccg cggtcgactg
     3361 caccgcgacc tcgtagatga gacggccgcc gtccttctcc ttcgccagca cgaagaacgc
     3421 gagctcgttc tcttcgtcga cgaagtggtt cacgaccttc cggccggtcc cggcgtggaa
     3481 cgccgcgtcg gcgagctgcc ggtagtcgcc gaccgtgtaa tagaacttct cgccgtcggc
     3541 gcgcacgtag tcgaccttgc gcatgttctt cgggtgcgag tcgatcaccc agatgaacgg
     3601 cgaggcgtcg gcggtcttgg tcttcgcgat gatcacgccg tggtccggga ggaacgagtc
     3661 gaacccctgc cggttcacga cctcgacgtc gtagaagtcg tacccgccgc ggtcgcactt
     3721 ggggtcgctg gactcacacg tcgagcggtc ctcgccgagg acgatccgga tgccgctgaa
     3781 cagagtgggg tccgcggtct ccggaggcga ctcccgctgc aggatgcggg cccggatcgg
     3841 ggcggcggac aacgcttcgc gattgaccgt cagcacctcg gccggcggca ggaagcccag
     3901 gcggatcttg ttgcggatca tgtggtgcgc gcccatcgtg gcgccgcccg tcggtgggat
     3961 cgtccaccgc tcccaggggc ccccgggtcc gttgaaggtg ccgcggctca tcatgtccca
     4021 gggcccggag tacgaccgga tcgggtcctc cgcgtacggg ttgttgtagt tgtcgagcac
     4081 gccgaggacg tggctgagct cgtgcgcgta gaccgagccg ccgtcgctct ctccctccgt
     4141 cgcgagcgcg ccggggagcg cgtgcgacca gatctgctgt gctgcgaaga acgacgtcca
     4201 gtcgacgtag cgcgtgcccg cccaattgcg ccagtcgggg aaggccggcg gcccgtactt
     4261 ctcggtcacg tcctccggct cggcgaagag catctggccg tagttgagcc acaccgtcga
     4321 ctcgtcgtac cccgcgtgga ggaggaagcg gaagtcgaac tgcccgtgct cctgcaccga
     4381 cgtcgtgacg tcggccagcg aagcctggac gagctccgtg tcgaagtccc cgtcgcaccc
     4441 gctgtcgtcg gggcagtcct cgctaccgga gaagtccgtg acgccgtact cgtacatgtt
     4501 gccttccatc gtgtaggggc cgaacccctc ggcgtcgacg tcgatcaggc cgtaggagtt
     4561 ctcgagccag tactcgccga cggtgtgccc gcgctgtgcg ttcttggggt tggtcgactt
     4621 gaacagcagg ttggtccagt acgccgcagg gtcttcgacg ccgagccccc gctggccggt
     4681 cgggtcgacc gtggtctccg cgacgcggaa cttctggtcc tggaagtcgc cgaggatcag
     4741 agcggcccgc agggtcttcg gcgcctcgta ctcgggattc ttccagtcgg tccccgggat
     4801 ccggcggtag tcgccccagg tcatgtcctc cgggacctcc cacgactgcg ggtcgatcgg
     4861 gcgggggagc ttcggcttcc gggcggggcc ctgtgcggtc ggtcgagcga cggcgggggc
     4921 cgcgcctccg atgagcaacg cggcgctgag cagcgccacg gtcgtacggc gtgcggtttg
     4981 catcccggcc tccgtgcagt cgacgatcgc cccgcgacga tatgcgcact tagcctgggt
     5041 tgctaaagga agcaagccca gccggccgaa gggatgaggt ggactactcg cccgcgcccg
     5101 aagagcgcgc ccggctcggg ccggtgaacc cgctgacggt agcggtcgcc gtcgtgctgc
     5161 tgtcggccgg cggcgccttc ggctggcgga tgaggacgag cgctccttcg gcgcgtccca
     5221 cgcccgaggc ggccgcggcg gcgctggcgc tcgagcgggt cgacggcgag ccgatgtgcg
     5281 gcccccgggg ccgcgacgac gtacgcgacg agatgctgcg cggagactcc ggccagctcc
     5341 cggagcgact cgccgagtac cttccgaaga ggtcgcccct gcccggttac gacgagacgt
     5401 cgtcgcatgc gatcgacccc gcctccgaga cggagttcgt ctcgggcgcc gtagacggcc
     5461 acgtggcgtc gttccgcccg gagggggagg ggggcttcga cgtatacgcg taccgcttcc
     5521 tcacgcgccg cgccgcggcc gacgccgtcg ccgacaac
//
LOCUS       DASQDO010000359         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_711188, whole genome shotgun sequence.
ACCESSION   DASQDO010000359 DASQDO010000000
VERSION     DASQDO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711188"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..828
                     /locus_tag="VEV43_10110"
     CDS             511..828
                     /locus_tag="VEV43_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23918.1"
                     /translation="MGVEEIREISKRVLGNRYRLEVAAAIGEQEPGVFHVRLLADKLR
                     LPDNKVRDELVKFSEAGLIQLLPKIRGQQDYRREESVFWAFAQEFVKELRTQGSTTSR
                     RRS"
     gene            complement(844..1257)
                     /locus_tag="VEV43_10115"
     CDS             complement(844..1257)
                     /locus_tag="VEV43_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23919.1"
                     /translation="MRVRVLAVALGVLAALGACDGGPGEPPVGVPSASPDEARAYEGL
                     EEVAEAADCEGLEDLGTANNAGLDEFGICHIGSANLDIYLTSQRGLWEHLADLYPSVL
                     GPNWIIVSPSGLEGARVVQERLGGELRVPPSPSPT"
     gene            complement(1307..1498)
                     /locus_tag="VEV43_10120"
     CDS             complement(1307..1498)
                     /locus_tag="VEV43_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018923527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23920.1"
                     /translation="MEETKKMSRAEAGRKGGRTTKERYGEEHFGRIGKIGGKKGGETT
                     KERYGSEFYQKIGRLGGSK"
     gene            1698..2780
                     /locus_tag="VEV43_10125"
     CDS             1698..2780
                     /locus_tag="VEV43_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYP23921.1"
                     /translation="MASVHDFLRYVVDKKGSDLHVKAGGPPYVRINGHLVRTEFPAMT
                     APDCERAAMELMNDDQARQFKAKGEVDFAYSEHGLGRFRVNVFRQRGSLGMACRRVLP
                     GSPAFETLGLPPVVKQLADEHRGMLLVTGPTSSGKTTTTGAIINHINATRSCHILTIE
                     DPIEILHPDRMAIVNQREIGHDTADFATALRAAMRQDPDVIFVGEIRDPETVKAALQA
                     AETGHFVISTLHTTDVSETVNRIIDFFQPHQQKQVRVSLSGSLKGIVSQRLIPRLDER
                     GRIPAVEVLVMNGRIHDLILNPEQTHMIHDIVAESSFYGMQTFDQALLALYRSGLVSL
                     DDAMRAATNSHDFTIALRQEGLQPVS"
     gene            2793..>4205
                     /locus_tag="VEV43_10130"
     CDS             2793..>4205
                     /locus_tag="VEV43_10130"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR02692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HYP23922.1"
                     /translation="MSVSFLSELLAEGSPVMDLAAHFEKAGHELYLVGGPVRDALLRR
                     AHTDLDFATDATPEQTIAIVKPLASNLWLQGREFGTVGAEISGLHMEITTFRTETYQP
                     SSRHPEVSFAADIETDLSRRDFTVNAMAIQLPGMNAVDPFGGKDDLAKKVLRTPIEPD
                     DSFSDDPLRMLRAFRFASQLDFQIAPEVLDSIERLREQLLTVSAERIRDELSKLLVGA
                     APGRALELADRTGLTDLFLPELSSLKLEQDPVHRHKDVFRHTLAVVERTAPIVVLRLA
                     ALLHDVGKPKTRRIGPEGVSFHHHEVVGAKMAEERLRALRFPNEIVEQVRTVIFLHHR
                     FHTYRLGWNDSAVRRYVRDAGPLLGTLNSLVRADCTTRDAAKAKRLAARMDELEERIA
                     ELAAREELEKIRPDLDGFQVMAYLGLPGGPTIKKALDHLLEVRLDEGPLSQDEAFARL
                     DAWARAEGISPAGERLPPREK"
BASE COUNT          693 a         1440 c         1390 g          682 t
ORIGIN      
        1 ctcgagccag cagcggggcg gcaagtgtgt actcggaacc cgaatcaacg agagcggcca
       61 ccttcgtcgg tgctaccgat tcaccagtca gtcgcaccgc gaccacgggc cgaagcgttt
      121 cccgacccct tcgaccggcg ggatcctcga cgtaaggaaa tagccaagct ttccgcgcgt
      181 cggagcgcgg accgttagcc aaggccgacg agttcagctt ctccttcggc gggagaacgg
      241 ataatcgttg cgtccgtgat gtctcgatac ttgagggcca ttacgagagc atcgggagtt
      301 tccttcgcgt cgacgatctc gccgttcttg agtgcgaccc acattccggg gaattcgcgg
      361 agagcatcgg ctatgcggac tggctcgatc gggcgcctcc ggcgtctgaa cagcttgaat
      421 ggtcctccca ttgtcttcct actgtcagca tccggaaacc gggttctgat aaacggttta
      481 cggtttccgt aaaccgtata cgctggaggg gtgggcgtgg aggaaatccg tgaaatctcc
      541 aagagagtct tgggtaatcg ctaccggctc gaagtggcag cggccatcgg ggagcaagaa
      601 cctggggtct tccacgtgcg gctgctcgcc gacaaattga ggcttccgga caacaaggtc
      661 agagacgagc tggtgaagtt ctccgaggcc gggctgatcc aactgctccc gaagattcgt
      721 gggcagcagg actaccggcg ggaggaatca gtcttttggg ccttcgccca ggagttcgtg
      781 aaggagctgc ggacgcaggg gtctacgacc agccggcggc ggtcgtgaag ggggcgctgt
      841 cacctaggtc gggctcgggc tcggcgggac cctcagctcg ccccccagcc tctcctgcac
      901 cacccgggcc ccctcgaggc ccgagggcga cacgatgatc cagttcggcc ccaggaccga
      961 cgggtacagg tcggcgaggt gctcccagag cccccgctgc gaggtcaggt agatgtcgag
     1021 gttcgccgag ccgatgtggc agatcccgaa ctcgtcgagc ccggcgttgt tcgcggtccc
     1081 taggtcctcg agcccctcgc agtcggccgc ctcggcgacc tcctcgaggc cctcgtaggc
     1141 ccgggcctcg tccggcgacg ccgacgggac gcccaccgga ggctctcccg gcccaccgtc
     1201 gcaggcgccg agggccgcga ggactcccag ggctaccgcc aggacccgga cgcgcaagag
     1261 acctcccccc ggcgtgaagg gaaggtctcg cgcgaagcct gggtggttac ttggagccgc
     1321 cgaggcggcc gatcttctgg tagaactccg agccgtagcg ctccttggtg gtctcgccgc
     1381 ccttcttgcc gccgatcttg ccgatgcggc cgaagtgctc ttccccgtag cgctctttcg
     1441 tcgtgcggcc acccttacgg ccggcctctg ccctgctcat cttcttggtc tcttccatcg
     1501 gatgaaccct tcccccggca ccttggtccg gaacgactgc gcctggcgga gcttgcttac
     1561 cctcgcccac acgccgcgaa acggagcaag ttcgacgaat cccgggccgg cggagactca
     1621 atacgtcgcc cggcgttgcc gaaggtgtac gtgacaggac ctcccgcccc gcgaccagag
     1681 gaacgactcc taccgagatg gccagcgtcc acgacttctt gcgctacgta gtcgacaaga
     1741 agggctccga cctgcacgtc aaggccggcg gccctcccta cgtcaggatc aacgggcacc
     1801 tcgtccggac ggagttcccg gcgatgacgg cccccgactg cgagcgggcc gcgatggagc
     1861 tcatgaacga cgaccaggcc cgccagttca aggccaaggg cgaggtcgac ttcgcgtact
     1921 cggagcacgg cctcggccgc ttccgagtca acgtcttccg tcagcggggg tcgctcggca
     1981 tggcctgccg gcgtgtcctc cccgggagcc cggccttcga gacgctcggc ctaccgccgg
     2041 tcgtcaagca gctcgccgac gagcaccgcg gcatgttgct cgtcacggga ccgacgtcgt
     2101 ccggcaagac gacgacgacc ggggcgatca tcaaccacat caacgccacg cgctcctgcc
     2161 acatcctcac catcgaggac ccgatcgaga tcctgcaccc ggaccggatg gcgatcgtga
     2221 accagcggga gatcggccac gacaccgcgg acttcgcgac cgcgctccgt gccgccatgc
     2281 gtcaggaccc ggacgtgatc ttcgtcggag agatccgcga ccccgagacc gtcaaggcgg
     2341 cgctgcaggc ggccgagacg ggccacttcg tcatctcgac gctccacacg acggacgtct
     2401 ccgagacggt caaccgcatc atcgacttct tccagccgca ccagcagaag caggtgcgtg
     2461 tctccctctc ggggtcgctc aaggggatcg tgtcgcagag gctcatcccg cggctggacg
     2521 agcggggccg gatcccggcg gtggaggtcc tcgtgatgaa cgggcgcatc cacgacctga
     2581 tcctcaaccc cgagcagacg cacatgatcc acgacatcgt tgcggagtcg tcgttctacg
     2641 ggatgcagac gttcgaccag gcgctgctgg cgctgtaccg gtcgggcctc gtctccctgg
     2701 acgacgcgat gcgtgccgcg acgaacagcc acgacttcac gatcgctctg cggcaggagg
     2761 gtctgcagcc cgtctcgtag ggtgaggccg acatgtcggt ctctttcctg agcgagctgc
     2821 tggcggaggg ttcgccggtg atggacctcg ccgcccactt cgagaaggcg gggcacgagc
     2881 tgtacctcgt cgggggtccc gtgcgcgacg cgctgctgcg ccgggcgcac accgacctcg
     2941 acttcgcgac ggacgcgaca cccgagcaga cgatcgcgat cgtgaagccg ctcgcgagca
     3001 acctgtggct ccagggccgc gagttcggga ccgtgggggc cgagatctcg ggtctccaca
     3061 tggagatcac gacgttcagg accgagacgt atcagccttc gtcgcgccat cccgaggtct
     3121 cgttcgccgc cgacatcgag accgatctct cgcgccgcga cttcaccgtc aacgcgatgg
     3181 cgatccagct cccggggatg aacgccgtcg acccgttcgg gggcaaggac gacctcgcga
     3241 agaaggtcct gcgcaccccg atcgagcccg acgactcgtt ctcggacgac ccgttgcgga
     3301 tgttgcgggc gttccgcttc gcatcgcagc tcgacttcca gatcgctccc gaggtgctcg
     3361 actcgatcga gcgcctgcgc gagcagctgc tcacggtgtc ggccgagcgg atccgcgacg
     3421 agctgtcgaa gctgctcgtg ggggccgcgc cggggcgtgc gctcgagctc gccgaccgca
     3481 ccgggctcac cgatctcttc ctcccggagc tctcgtcgct gaagctcgag caggatcccg
     3541 tgcaccgtca caaggacgtc ttccggcaca cgctcgcggt cgtggagcgg acggctccga
     3601 tcgtcgtgct gcgtctcgcc gcgttgctcc acgacgtcgg caagccgaag acgcgccgca
     3661 tcgggccgga gggagtcagc ttccaccacc acgaggtggt cggggcgaag atggccgagg
     3721 agcgccttcg cgccctgcgg ttcccgaacg agatcgtcga gcaggtgcgg accgtgatct
     3781 tcctgcacca ccgcttccac acttaccggc tggggtggaa cgactctgcc gtgcgccgct
     3841 acgtacgcga cgcggggccg ctgctgggga cgctcaactc gctggtgcgc gccgactgca
     3901 cgacgcgcga cgccgcgaag gcgaagcggc tcgcggcccg gatggacgag ctcgaggagc
     3961 ggatcgccga gctcgcggcc cgcgaggagc tcgagaagat ccggcccgac ctcgacgggt
     4021 tccaggtcat ggcgtacctg gggcttccgg gggggccgac gatcaagaag gcgctcgacc
     4081 atctgctgga ggtccgcctc gacgaggggc ctctgtcgca agacgaggcg ttcgctcgat
     4141 tggacgcatg ggcgcgggcg gagggcatct cgcctgccgg tgagcggctg ccgccccgcg
     4201 agaag
//
LOCUS       DASQDO010000360         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1200922, whole genome shotgun sequence.
ACCESSION   DASQDO010000360 DASQDO010000000
VERSION     DASQDO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200922"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /gene="murA"
                     /locus_tag="VEV43_10135"
     CDS             <1..1045
                     /gene="murA"
                     /locus_tag="VEV43_10135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYP23923.1"
                     /translation="VSSAASGELSEETPYELVTRMRASIQVLGPLLARLGRARVAMPG
                     GDAIGSRPIDLHLQGLERMGAKFTSQHGYVEGVAHRLRAADVLLEFPSVGATENLLMG
                     AVLAEGRTTIENAAREPEIQDLAAFLNACGARIEGAGTPFISIDGVDALGGVRHEIIP
                     DRVESGTFAIAAAATRGDVRLVDTRPEFFELPLQKLEQAGAEVDRGDGEVRVRMGGRP
                     QSLDLSTLPYPGFPTDLQPPMMVLLAGADGSSILTENVFEARFLFVDELNRMGCRIRV
                     EGHHAVIRGIERLSGAPVRAPDIRAGAALVLGGLCAEGRTEVYDTGHVERGYERFVEK
                     VNGLGGSLTLLDD"
     gene            1073..2710
                     /locus_tag="VEV43_10140"
     CDS             1073..2710
                     /locus_tag="VEV43_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HYP23924.1"
                     /translation="MSRRLVVALLAAFVALSLPAPVQAARTPGALVGPVRLVPRGSGP
                     IHVSGLHGYFGQVGIAPASDGLVVTNRLPLERYLLGLQEVPPSWPDEALRAQAVAART
                     YALWTLSRPRAGAAATYGFDICASVECQVFSGADVVATPDGQRWAAAVQSTADEAVLY
                     EGNPILARYHSTSGGATLDNPQAFPGEPDYPYLQGVPSRTEQGSPLYRWTVRFPLQHL
                     QAMLTSGGLWTGAHGQMRRAVTVRSSSGLHYPDVVLTGTRKNLRVDAESVRDIVRTQG
                     PALYPGVYPSRWFTTSGRLPETLPANRISIVTRKGVAVVTGRGWGHGTGMSQWGAHGL
                     AEQGASYREILAHYYTGTTVGEVDAPASISVGVAWARSSVVATGAFDIVDGRGDVAVR
                     DALGSWTFTPGGGGVVRVDPPEGHSLPLRVGIVAAPKRAEPGERVPITVALSKPARLQ
                     VGSGDASVHRAGRGRVFWKAPRDPGSYEVTLVASSGPGESQERRLDIRVVAQEGATSA
                     PIPPREGDGGVGGGLLRLLVGAVLIGLVIAGLGSRIK"
     gene            2710..2952
                     /locus_tag="VEV43_10145"
     CDS             2710..2952
                     /locus_tag="VEV43_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HYP23925.1"
                     /translation="MSGALTVEDRVREALEAIRPAIQMDGGDIELVGIHDGTVTVRLL
                     GTCEACPISPVTLRHGVERVLRERVPGITDVVAIEA"
     gene            2960..3892
                     /gene="meaB"
                     /locus_tag="VEV43_10150"
     CDS             2960..3892
                     /gene="meaB"
                     /locus_tag="VEV43_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HYP23926.1"
                     /translation="MELDELIRRALAGERRAIARTISLVEDGGADLGRVMQELYPHTG
                     SAYSIGITGAPGAGKSTLTERLVGRARAGSLTVGVLAIDPSSPFSGGALLGDRVRMQS
                     HATDPDVFIRSMATRGHLGGISLATPEAFRVLDACGKELVVIETVGVGQAEVEITDAC
                     DTTLVVVNPGWGDAVQAAKAGLMEIADVFVVNKADRNGTRQAVQELRQMLELSETSWK
                     PEIVETVATKGTGIDELWAAIEKHRAYQEEQGLLEARRRRRVQREIKEIVAERYRTRV
                     EHDEVALLNELTDEVAARRLDPYAAADRVVAALS"
     gene            4027..4212
                     /locus_tag="VEV43_10155"
     CDS             4027..4212
                     /locus_tag="VEV43_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23927.1"
                     /translation="MDRIKRRIALCLAASALASVGMAAPASAGDSCHPLPQAYCNAIQ
                     YLNDCVFWPPPAEYCDN"
     gene            4270..4467
                     /locus_tag="VEV43_10160"
                     /pseudo
     CDS             4270..4467
                     /locus_tag="VEV43_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571404.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
BASE COUNT          642 a         1569 c         1610 g          646 t
ORIGIN      
        1 cgtctcgagc gccgcgtcgg gcgaactgtc ggaggagacg ccctacgagc tcgtcacgcg
       61 gatgcgggcc tcgatccagg tcctcggccc cctcctcgcg cggctcggcc gtgcccgggt
      121 ggcgatgccc ggcggcgacg cgatcgggtc gaggccgatc gacctgcacc tgcaggggct
      181 cgaacggatg ggcgcgaagt tcacctcgca gcacggctac gtcgaaggcg tcgcgcaccg
      241 gctgcgcgcc gccgacgtcc tgctcgagtt cccgtccgtc ggcgcgaccg agaacctgct
      301 catgggcgcg gtgctcgcgg agggccggac gacgatcgag aacgcggcgc gcgaacccga
      361 gatccaggac ctcgccgcct tcctcaatgc ttgcggcgcg cgcatcgaag gggccgggac
      421 gccgttcata tcgatcgacg gcgtcgacgc gctcggtggc gtgaggcacg agatcatccc
      481 cgaccgcgtg gaatccggga cgttcgcgat cgccgccgcc gcgacgaggg gcgacgtccg
      541 tctagtcgac acgcgtccgg agttcttcga gctccccttg cagaagctcg agcaggcggg
      601 ggccgaggtc gaccgcggcg acggcgaggt gcgggtcagg atggggggcc ggccgcagtc
      661 gctcgacctc tcgacgctcc cgtatcccgg gttcccgacg gacctgcagc cgccgatgat
      721 ggtcctgctc gcgggggcgg acggctcgtc gatcctcacc gagaacgttt tcgaggcgcg
      781 ctttctgttc gtggacgagc tgaacaggat ggggtgccgg atcagggtcg aggggcacca
      841 cgcggtgatc cgcgggatcg agcgtctctc cggcgccccc gtccgagcgc ccgacatcag
      901 agcgggcgcg gccctcgtcc tcggcgggct ctgcgcggag gggcgcaccg aggtgtacga
      961 caccggccac gtagagcgcg gctacgagcg gttcgtcgag aaggtcaacg gcctcggggg
     1021 ctccctgacg ctgttagacg actagcccct tcctagacct atcgtgaacg gcgtgtcccg
     1081 ccgtctcgtc gttgccctgc tcgccgcctt cgtcgcgctc tcgcttcccg cgccggtgca
     1141 ggctgcgcgc acgcccggcg cgctcgtcgg tccggtgcgg ctcgttcccc gcgggtccgg
     1201 cccgatccac gtgtccggcc tccacggcta cttcggccaa gtcggcatcg cgcctgcgag
     1261 cgacgggctc gtcgtgacga accggctccc gctcgagcgc tacctcctcg gcttgcaaga
     1321 ggtcccgccg agctggcccg acgaggcgct ccgcgcgcag gcggtcgccg cgcggacgta
     1381 cgcgctgtgg accctgtcgc gtccgcgcgc gggggccgcg gcgacgtacg gcttcgacat
     1441 ctgcgcgtcg gtcgaatgtc aggtgttctc gggagcggac gtcgtcgcga cgccggacgg
     1501 acagagatgg gccgctgccg tccagtccac cgcggacgaa gcggtcctgt acgagggcaa
     1561 cccgatcctc gcccggtacc actcgacgtc cggcggcgcc acgctcgaca acccgcaggc
     1621 gttcccgggt gagcccgact acccgtacct acagggcgtg ccgagccgga ccgagcaagg
     1681 gtccccgctg taccggtgga ccgtcaggtt cccgctgcaa cacctccagg cgatgctgac
     1741 gagcgggggg ctgtggacgg gggcgcacgg gcagatgcgc cgcgccgtga ccgtgcgatc
     1801 gtccagcggc ctccactacc ccgacgtcgt gctcaccggc acccgcaaga acctgcgcgt
     1861 cgacgccgag agcgtgcgcg acatagtcag gacgcaggga cccgcgctct atcccggcgt
     1921 gtacccgtcg cgctggttca cgacgagcgg ccgtctgccc gagacgttgc ccgcgaaccg
     1981 gatctcgatc gtcacgcgca aaggcgtcgc ggtcgtcacc ggacgcgggt gggggcacgg
     2041 gaccgggatg tcgcagtggg gcgcgcacgg gctcgcggag cagggcgcga gctaccgcga
     2101 gatcctcgcc cactactaca cgggcacgac cgtcggggag gtcgacgcgc ccgcgtcgat
     2161 ctccgtcggc gtcgcgtggg cccgttcttc ggtggtcgcg accggcgcgt tcgacatagt
     2221 cgacggtcgc ggcgacgtgg cggtgcgcga cgcgctcggc tcgtggacgt tcacgcccgg
     2281 cgggggaggg gtcgtccgcg tcgatccccc ggaaggtcac tcgctgccgc tgcgggtcgg
     2341 gatcgtcgcc gcgcccaaga gggccgagcc cggcgagcgc gtgccgatca cggtcgcgct
     2401 gtcgaagccg gcgcggctgc aggtcggctc cggtgacgcg tcggttcacc gagcgggccg
     2461 gggcagggtg ttctggaagg cgccccgtga tcccggctcc tacgaggtca cgcttgtcgc
     2521 gtcgtccggg ccgggcgagt cccaggaacg tcgcctcgac atccgcgtgg tcgcccagga
     2581 gggggcgacc tcggccccca tccccccccg ggaaggagac ggcggcgtgg gcgggggcct
     2641 gctgaggctg ctcgtcggag ccgtgctgat aggcctcgtg atcgcggggc tgggaagtag
     2701 gatcaagtga tgagcggcgc acttacggta gaggaccgcg tccgcgaggc gctcgaggcg
     2761 atccggccgg cgattcagat ggacggcggc gacatcgagc tcgtcggcat tcacgacggg
     2821 accgtgaccg tgcgcctcct gggcacctgc gaggcgtgcc cgatctcgcc cgtcacgttg
     2881 cgccacggcg tcgagcgggt cctgcgcgag cgcgtccccg gcatcaccga cgtcgtcgcg
     2941 atcgaggcct agccccgcct tggagctcga cgagctgatc aggcgcgccc tcgcgggcga
     3001 gcggcgggcg atcgcccgca cgatctccct cgtcgaggac ggcggcgccg acctcggccg
     3061 cgtgatgcag gagctctacc cccacaccgg gtcggcctat tcgatcggta tcacgggcgc
     3121 accgggggcc ggcaagtcca cgctgacgga acgtttggtc ggccgggccc gggcgggttc
     3181 tttgaccgtg ggagtgctcg ccatcgaccc ttcgagcccc ttctcgggag gcgcgctcct
     3241 cggcgacagg gtccgcatgc agagccacgc caccgacccc gacgtgttca tccgctcgat
     3301 ggcgacccgg ggccacctcg gcgggatctc gctcgcgacg cccgaagcgt tccgcgtgct
     3361 cgacgcgtgc gggaaggagc tcgtcgtgat cgagaccgtc ggcgtcggcc aggccgaggt
     3421 cgagatcaca gacgcgtgcg acaccacgct cgtcgtggtc aacccgggtt ggggcgacgc
     3481 cgtgcaggcg gcaaaggccg ggctgatgga gatcgccgac gtgttcgtcg tgaacaaggc
     3541 ggatcgcaac gggacgcgcc aggccgtcca ggagctccgc cagatgctcg agctctccga
     3601 gacttcgtgg aagcccgaga tcgtcgagac ggtagcgacg aaggggaccg ggatcgacga
     3661 gctgtgggcc gcgatcgaga agcaccgggc ctatcaagag gagcaggggt tgctcgaggc
     3721 caggcgccgc cgccgggtgc agcgtgagat caaggagata gtggcggagc gctaccggac
     3781 gagggtcgag cacgacgagg tagcgctact taacgagctg acggacgaag tagcggcgag
     3841 gcgtctggac ccctacgcgg ccgcggaccg cgtggtcgcc gccctctcct gacctcttcg
     3901 tcgcgctatc gcgtacgcat accgacggtt ctgcgccaac gaacccgcgt gggcaccctg
     3961 aaggagaccc gcggccccgt cccgaatacc ccatatgtcc gagttgggga gaccaaggag
     4021 gctctggtgg accggatcaa gcgacggatc gcgttgtgtc ttgcagcgtc ggcgttggcg
     4081 agcgttggca tggccgcgcc ggcctcggcc ggcgacagct gccatcccct gccccaggcg
     4141 tactgcaacg cgatccaata tctgaacgat tgcgtcttct ggcccccgcc cgccgagtac
     4201 tgcgacaact aggcggcccg gctacctgcg tccgaaggcg cctgcacggg cgcaggtaga
     4261 atccgcccca tgacagacga ctccatggct cgttggcgcg agcagtacga ccaggccaag
     4321 ctccgcgacg acgccgactt cgacaccctc tccagcgtcg agctcgagcc cctctacggc
     4381 cccccgacgg aagcgtcccc gagaccatcg gcgtccccgg tgagtatccg ttcacccccg
     4441 gcatctaccc gagcgggtac cgcggcc
//
LOCUS       DASQDO010000361         1935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_466618, whole genome shotgun sequence.
ACCESSION   DASQDO010000361 DASQDO010000000
VERSION     DASQDO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466618"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..695
                     /locus_tag="VEV43_10165"
     CDS             129..695
                     /locus_tag="VEV43_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23928.1"
                     /translation="MSVALLALAGCGGGDEGGEGGGDATFDAGEAVIAEPGSTRALGE
                     RAFVEYEGIGPKFEPDAKTTLAVTVTKVDEGDNSDIDGLGESTVPFYVFLEYENHGEG
                     GIKVLGIDSHLSAEGSDGEEYNTEGVIEISGEFEQCPDADPDATLAPEQAINDCVVVM
                     LAEGVSLKQVRFMGNPISQDEPIGWTVG"
     gene            727..1290
                     /locus_tag="VEV43_10170"
     CDS             727..1290
                     /locus_tag="VEV43_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23929.1"
                     /translation="MSRRLILVLGGLVALTAAMIGIAQLTGGGDELHPLGTEVVVGLH
                     EPKEDGSEGERTKLGLTVVAVRRGTQEELEANGIEVEPEDRSATPYYVDARFTNRGPG
                     TVEHAPSVALDDSDGNSFRGLIIIGAGDEPFEPCPTRTDAQLEPGESDESCSLILVPD
                     GLDVARVSYLSDRGPSEEPEWVYWASE"
     gene            1427..>1935
                     /locus_tag="VEV43_10175"
     CDS             1427..>1935
                     /locus_tag="VEV43_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23930.1"
                     /translation="MFAAGVWVAAGGRGSRFERQEELWGGLLFATFGLLALYVVLGVW
                     GEDDDHPRLRGPTLSPVGEPIRRGGEIRVVLGGRIPADERLEVGLICAERVETVVHSN
                     AAIVRETAVTTVLEEWQSVAPRAGEHIATFRLPPDAPYSYEGECVSFAWRIAARAVRQ
                     GRPDAWLEEP"
BASE COUNT          292 a          638 c          713 g          292 t
ORIGIN      
        1 ccgaggtcgc gcgcgacctc gactagcggg ctatcagccc ctagcgcagc ccggtagccc
       61 gagccgtccc accgtagtat cgtcgcccca tgcggcggct cggtggacga cggggacgtg
      121 cgctcggggt gtctgtcgcg ctcctggctc tcgccggctg cggcggtggc gacgagggtg
      181 gcgagggcgg cggggacgcg accttcgacg caggtgaggc cgtcatcgcc gaaccgggaa
      241 gcacgcgggc cctgggcgag cgcgccttcg tcgagtacga gggcataggc ccgaagttcg
      301 agcccgacgc caagaccacg cttgccgtga ccgtgacgaa ggtcgacgag ggagacaact
      361 cggacatcga cggtctcggc gagagcacgg ttcccttcta cgtcttcctc gagtacgaaa
      421 accacggcga gggcggcatc aaggtgctcg ggatcgactc gcatctctcg gcggaaggct
      481 ccgacggcga ggagtacaac accgagggtg tgatcgagat cagcggcgag ttcgagcaat
      541 gcccggacgc cgatccggac gcgacgctcg cgcccgagca ggcgatcaac gactgcgtgg
      601 tcgtgatgct cgccgagggt gtgtctctca agcaggtccg cttcatgggg aacccgatct
      661 cccaggacga gccgatcggg tggacggtcg gatgatcgct tcctcttcct agggcgcgtt
      721 ccggacgtgt cgcgccggct catcctcgtg ctcgggggcc tggtggcctt gacggccgcg
      781 atgatcggga tcgcccagct cacgggcgga ggggacgagc tccacccgct cggcaccgag
      841 gtcgtggtcg gcctccacga gccgaaggag gacggatccg agggcgagcg aacgaagctt
      901 gggctcaccg tcgtcgccgt tcgtcgcggc acgcaggagg agctcgaggc caacgggatc
      961 gaggtcgagc ccgaagaccg gagcgcgacc ccgtactacg tcgacgctcg gttcacgaac
     1021 aggggcccgg gcacggtcga gcacgctccg agtgtcgctc tcgacgactc cgatggaaac
     1081 agctttcgcg gactgatcat catcggcgcg ggtgacgagc ccttcgagcc ttgccccaca
     1141 aggacagacg cccagctcga gcccggggag agcgacgaga gttgctcgct catcctcgtc
     1201 cccgacggtc tggacgtcgc gagggtctct tacctgagcg accgagggcc ctccgaggag
     1261 ccggagtggg tctactgggc gtctgagtga gacgcccgcc ggtcatcggt tgaggcgacg
     1321 cccgttcccg cacctgccgg ccggccggga gccgggcccg cagacgctgc gaacgcggcg
     1381 gaggcgagat gcgctctccc ccctcgtcgg cgtcgtgggc acggcgatgt tcgccgcggg
     1441 cgtgtgggtg gctgcagggg gccgcgggtc gcgtttcgag cgccaggagg agctctgggg
     1501 cgggcttctc ttcgcgacgt tcggcctgct cgccctctat gtcgtgctag gcgtctgggg
     1561 agaggacgac gaccatcccc gcctgcgtgg gccgacgcta tcccccgtgg gcgagccgat
     1621 ccggcgcggc ggcgagatcc gcgtcgtcct aggcggtcgc attcccgctg acgagcggct
     1681 ggaggtcggc ctcatctgcg ccgagcgggt ggagaccgtg gttcactcga acgccgcgat
     1741 cgttcgggag acggccgtga cgacggtgtt ggaggagtgg cagtccgtcg cgccccgagc
     1801 gggcgagcac atcgccacct tccgcctgcc gcccgacgcg ccctactcct acgagggtga
     1861 gtgcgtctcg ttcgcctgga ggatcgccgc acgggccgtc aggcaaggaa ggcccgacgc
     1921 ttggctcgag gagcc
//
LOCUS       DASQDO010000362         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_589593, whole genome shotgun sequence.
ACCESSION   DASQDO010000362 DASQDO010000000
VERSION     DASQDO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589593"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..880)
                     /locus_tag="VEV43_10180"
     CDS             complement(56..880)
                     /locus_tag="VEV43_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYP23931.1"
                     /translation="MRPRIQCSTGPFWAFSLEVAMDSLAEAGFEEIELMVTRDPRTQD
                     PEIPKRLADERGLKITSVHGPFLVVTKNVWGLDPIGKIRRGAEMCRALGAETMIVHPP
                     FVWEREYTRWAGEEAEAFEAETGVAVAVETMYPVWMAGRRLRAYRWLDPRELFAACHR
                     VALDTSHVTVSRGDIIDVYSLLRPKLVHVHLSDNAADGRDGHLSLGDGVLPIDRFLAE
                     LRRTDYSGAISLELSVSRFLETPKELVGMLRRNREYVEDRLFRKARVAKGLPRQTR"
     gene            1187..2656
                     /locus_tag="VEV43_10185"
     CDS             1187..2656
                     /locus_tag="VEV43_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYP23932.1"
                     /translation="MNVAELLDRSAAEYPDKPALLTPNGETVTFAELRERAGSVAAWV
                     AAAGIRPGGRVGIAMHNVPEFVSSYFGVLRVGGVVIPLNPTLTPPEVERVLDDSGAAI
                     VVGVSPAGDAASAACEETGVRYVDAANPPAPGSPVATHPAEDDELAVLAYTSGTTGEP
                     KGAMLTHGNLLANLEQQMSIPDARVEPDDVLLLSLPLFHIFGLNVTLGLLVRTAATGV
                     LTDRFEPVECLRLIQEHRVSVMFGAPTMYTALASTPGADQYDLSSVRLAISGAAPLQP
                     EVLARFKELFGVDVYEGYGLTETSPTLTSNRMAEAPRPGSIGKPLPGVEIRLVDEAGE
                     DVELGDPGEILARGPNVFKGYWNKEDETERAFAGEWFRTGDVAVQDEDGYLYIVDRKR
                     DMVIVSGFNVFPSEVEHALTQNPSVAEAAVVGVPHAYTGEAVKAFVVLEPGASVTPDE
                     IVADVQSRLARFKCPREVEIVDALPHLLTGKVLRRALRT"
     gene            complement(2619..>3154)
                     /locus_tag="VEV43_10190"
     CDS             complement(2619..>3154)
                     /locus_tag="VEV43_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYP23933.1"
                     /translation="LVGRDADGGETILGLCLPGEVAGAVAAVDARPHPYDYVAATAVA
                     ALGVDARLFVAVVSANPDACLALTRLFTRRVRWLCDATLERTSGGVPSRLAGRLLDLA
                     EMLGRQRGSGIDVELPFPQRDLGGLAGMCRESACKTLSRWKAEGTVDYEGRRLRILRP
                     DVLSRIRCAGRAARPSP"
BASE COUNT          465 a         1139 c         1093 g          457 t
ORIGIN      
        1 ggtcacaggc gtccctccca gtggttccgc ctcccggtct cggccgggaa acggctcaac
       61 gcgtctgccg cggcaggccc ttggccacgc gggccttgcg gaagagcctg tcctcaacgt
      121 attccctatt tcttcgaagc atcccaacca gctctttagg ggtctcgagg aatcggctca
      181 cggagagctc cagcgagatc gcccccgagt agtccgtgcg gcgcagctcg gccaggaatc
      241 ggtcgatcgg cagcaccccg tcgccgagcg agaggtgccc gtcccggccg tccgcggcgt
      301 tgtcggagag atggacgtgg acgagcttcg gccgcagcag cgagtacacg tcgatgatgt
      361 cgccccggga gaccgtgacg tggctcgtgt ccagggcgac ccggtggcag gcggcgaaga
      421 gctcgcgggg gtcgagccac cggtaggcac ggagccgccg ccccgccatc cacaccgggt
      481 acatggtctc gacggcgacg gccacgccgg tctcggcctc gaaggcctcg gcctcctcgc
      541 cggcccagcg ggtgtactca cgctcccaga cgaacggcgg gtgcacgatc atcgtctcgg
      601 cccccagcgc ccggcacatc tcggcgccgc ggcggatctt cccgatgggg tcgagccccc
      661 agacgttctt ggtgacgacg aggaaggggc cgtggaccga ggtgatcttg aggccgcgct
      721 cgtcggcgag ccgcttcggg atctcggggt cctgggtccg ggggtcccgg gtgaccatga
      781 gctcgatctc ctcgaagccg gcctcggcga gcgagtccat ggcgacctcg agcgagaacg
      841 cccagaaggg gccggtcgag cactggatcc ggggcctcat gggtccgatg gtcccacgcc
      901 cgacgcgaaa aggcccccgc cgaagcgggg gccctctcgt ggagccgaag ccggtgaggc
      961 ggcttggggc gggctgggag gctcgagaca cgacccggcg tgaggatcgt gcggtcctta
     1021 cgtcccaccg tctttcagct ccaaggtaat ggtcggccgt ccgacgactt tcttgagtgt
     1081 gcttccagaa aattccttcg cgtaccgagc ccgaatcttt gctgttcgct agctagtgct
     1141 tgctacagtt tgcactccac gcttcccggg gagacaatca gacgagatga acgttgccga
     1201 gctactcgac cgctccgccg ccgagtaccc ggacaagccc gcgctcctca cgccgaacgg
     1261 cgagaccgtg acgttcgcgg agctccggga gcgggccggg tccgtcgccg cctgggtcgc
     1321 cgcggccggc atccgtcccg ggggccgagt cgggatcgcg atgcacaacg tgcccgagtt
     1381 cgtgtcgtcg tacttcggag tgctgcgggt gggcggtgtg gtcatccctc tgaacccgac
     1441 gctcacgcca cccgaggtcg agcgggtgct ggacgactcc ggcgctgcga tcgtcgtcgg
     1501 cgtcagcccc gccggggacg ccgcgagcgc cgcgtgcgag gagaccggag tccgctacgt
     1561 cgacgcggcg aacccgcccg cccccggctc tcccgtcgcc acgcaccccg ctgaggacga
     1621 cgaactcgcc gtcctcgcgt acacgtcggg gacgaccggc gagcccaagg gagcgatgct
     1681 cacgcacggc aacctcctcg cgaacctcga acagcagatg tcgatccccg acgcccgcgt
     1741 cgagccggac gacgtgctgc tgctgtcgct cccgctcttc cacatcttcg ggctcaacgt
     1801 cacgctgggc ctcctcgtgc gcaccgccgc gaccggcgtg ctgaccgatc ggttcgagcc
     1861 ggtcgagtgc ctgcgcctga tccaggagca ccgcgtctcg gtcatgttcg gcgcgccgac
     1921 gatgtacacg gcgctcgcgt cgacgcccgg cgccgaccag tacgacttgt cgagcgtgcg
     1981 gctcgcgatc tcgggcgcgg cccccctgca acccgaagtg ctcgcccggt tcaaggagct
     2041 cttcggcgtc gacgtctacg agggctacgg cctcaccgag acctcgccta cgttgacctc
     2101 gaaccgcatg gcggaagcgc cgcgcccggg ctcgatcggc aagccgctgc ccggcgtcga
     2161 gatcaggctc gtcgacgagg cgggcgagga cgtcgagctc ggcgacccgg gcgagatcct
     2221 ggcgcggggg ccgaacgtct tcaagggcta ctggaacaag gaggacgaga ccgagcgcgc
     2281 cttcgccggc gagtggttcc gcaccggcga cgtcgcggtc caggacgagg acggctacct
     2341 ctacatcgtc gaccgaaagc gcgacatggt gatcgtgtcc gggttcaacg tcttcccttc
     2401 cgaggtcgag cacgcgctga cgcagaaccc gagcgtcgcg gaggccgcgg tcgtcggcgt
     2461 gccgcacgcg tacacgggcg aggcggtgaa ggcgttcgtg gtgctcgagc ccggagcctc
     2521 ggtgaccccc gacgagatcg tggccgacgt gcagagcagg ctggcgcgct tcaagtgccc
     2581 ccgcgaggtc gagatagtcg acgcgttgcc gcacctcctc acggggaagg tcttgcggcg
     2641 cgccctgcgc acctgatccg gctcagcacg tccggccgca ggatccgcag ccgccgtccc
     2701 tcgtagtcca cggtcccctc ggccttccac cggctcagcg tcttgcacgc gctctcccgg
     2761 cacatccccg cgaggccgcc gaggtcgcgc tgcgggaacg ggagctcgac gtcgatcccc
     2821 gagccgcgct gccgcccgag catctcggcg aggtcgagca gccggcccgc gagccgcgac
     2881 gggacgcccc cggacgtccg ctcgagcgtc gcgtcgcaca gccaccggac ccgccgcgtg
     2941 aacagcctcg tcagcgcgag gcacgcgtcg gggttcgccg acaccacggc gacgaacagc
     3001 cgcgcgtcga cgccgagcgc ggccaccgcc gtggcggcga cgtagtcgta cgggtgcggg
     3061 cgcgcgtcga cggcggcgac ggcacccgcg acctcgcccg gcaggcagag cccgaggatc
     3121 gtctcgccgc cgtcggcgtc acggcccacg agct
//
LOCUS       DASQDO010000363         7262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_773465, whole genome shotgun sequence.
ACCESSION   DASQDO010000363 DASQDO010000000
VERSION     DASQDO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773465"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /gene="gatB"
                     /locus_tag="VEV43_10195"
     CDS             <1..881
                     /gene="gatB"
                     /locus_tag="VEV43_10195"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYP23934.1"
                     /translation="MEEGSMRVDANVSVRRRGEKEFGAKVEVKNMNSIRSVGRALEYE
                     ERRQRDALDAGESLIQETRHFDEKSGTTSSLRTKEFAVDYGYFPEPDLVPFEPGDEWV
                     GKVRAELPELPAARRERFAQEYGLAEGDIAILTATKATADWFEEAARAYGGQAKKIVN
                     WIIADLFGLLNEAGIELGESKIAPRQLAGLVELVDSGKVSGKQAKLVLAGMFETGSDA
                     DAVAREKGLEQVSDTGAIEAAVDEVIAENADAADKVRGGQTNTIGFLVGQVMKKTRGQ
                     ANPGMVNDLLRKKLTS"
     gene            935..1366
                     /locus_tag="VEV43_10200"
     CDS             935..1366
                     /locus_tag="VEV43_10200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYP23935.1"
                     /translation="MTEVCSGPPESIWDLITDWEHQDDWMLEASDFVVVSDVREGVGV
                     EAEATIRIGGITTRDRVRVGEWDPPRRLVIEHLGWVTGRGIIELTPVAGRRTHVWWRE
                     ELSPPLGVLGAIGMTAFKPLMARVFRRDLRILAGLARAAAR"
     gene            1501..2670
                     /locus_tag="VEV43_10205"
     CDS             1501..2670
                     /locus_tag="VEV43_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23936.1"
                     /translation="MMALALTSAIPAGAAEQAEPVKSDNVTHVFNDQYFSGTELAASG
                     KYVYTGQMDGDESVDRGSAPEEGGIRIYDVSGKVPKKVGFLHCPGNDNDVEVVKPGLI
                     VVSFATNKCAPQAGNGLMTVDVKNPRKPKILGAVNTGKNHTHKPYPGTNYVYTAGGGL
                     SGGPGAGPAIVDVSDPKSPKVAGKLSTLTTDCHDISFRISKGSKLMFCAGAIGTGEVQ
                     IYDVSDPLAPALVSRIFNPAIQYSHYAVASSDGQFLAIDDEAFAAHECASGNSPTGRV
                     WIYNIENPQLPLLVGSFAPPRGGDDTVPVGHLQGWVPTWCLSHGLDWQPGTHNVAVTW
                     FTGGWSLINVDGQLPTEIAHYQAEDSATYSTLWHNGKLYTNDSFRGFDAFNLKLK"
     gene            complement(2836..4017)
                     /locus_tag="VEV43_10210"
     CDS             complement(2836..4017)
                     /locus_tag="VEV43_10210"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018842015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYP23937.1"
                     /translation="MSTRVGAITESSTLALDAKAKALKAAGENVIGFGAGEPDFPTPA
                     HIVEAAAEAARHTVYHRYTPAPGLPELRAAIARKTQRDSGYAVQPEQVLVSNGGKHAL
                     FNAFLTLLDEGDEVIVPAPYWVSYPEVIRLAGATPVEIPTTEATGFKVSLDDLERALT
                     PRTKMLLFVSPSTPTGAVYSPEEVRAIGEWAVERGIWVLTDEIYEHLVYGDAKFASMP
                     VEVPEIANHCVVVNGVAKTYAMTGWRVGWMIAPADVTKAAASLQSHATSNVSNVAQAA
                     ALAAVSGDLQAVYEMREAFDRRRVTIHKMLNEIPGVICPEPEGAFYVFPSFAEWIGRD
                     VGGVTIESAAHLAEVILERAKVAIVPGEGFGAPGYARLSYALGDDDIREGVGRIADLL
                     R"
     gene            4098..5213
                     /gene="serC"
                     /locus_tag="VEV43_10215"
     CDS             4098..5213
                     /gene="serC"
                     /locus_tag="VEV43_10215"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HYP23938.1"
                     /translation="MPSDILIPPALRPSDGRFGSGPSKVRPEAVAALAAAAPGYLGTS
                     HRQKGVKSVVGRLREGLATLFGLPNGYEVVLGNGGATAFWDALAFNLVEGRSRHYVFG
                     EFSAKCAAAVRAAPHLHEPEVLEAEPGSHPEPAPSTAADLYALTHNETSTGVAMPVRR
                     VAPQGLTVVDATSAAGGLPVDPAQFDVYYFAPQKCFASDGGLWIGLFSPAALERIESV
                     AKSGRYVPASLDLAIAVENSRKDQTYNTPALATLFLMAEQLDWMLANGGLEWTVGRCA
                     RSADLLYAWAEASDVAAPFVRDPAQRSAVVGTIDFADGVDAKAIASALRANGIVDTEP
                     YRKLGRNQLRVAMYPAIEPDDIEALTRCIDYVVGELA"
     gene            5210..5713
                     /locus_tag="VEV43_10220"
     CDS             5210..5713
                     /locus_tag="VEV43_10220"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP23939.1"
                     /translation="MKARPAAPDDAPAIARVHVASWNAAYRGVISDEALDELTEERLA
                     PEWRREIETGESEVAVVEVDAEVAGYSRYGPSRDDDLDPATTAEIYGFYLHPDTWGRG
                     AGRVLMTHVKDDLGTRGFRDAVLWVVQVNDRAQAFYRAVGFAPDGRDDKLCIGAPEYR
                     YGGELPS"
     gene            5727..>7262
                     /gene="serA"
                     /locus_tag="VEV43_10225"
     CDS             5727..>7262
                     /gene="serA"
                     /locus_tag="VEV43_10225"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HYP23940.1"
                     /translation="MRPVKVLVSEKLSAAGVERLREHVQVDVRPGLSPDELLGVIPDY
                     DALIVRSGTKVTAEVLEAGKSLKVVGRAGIGLDNVDVDAATRHGILVVNAPQSNVLSA
                     AEHTVALMLAQARNIPQAHSALVSGSWERERWQGVELHGKTLGILGLGRVGTLVAQRA
                     SAFGMRLVAYDPYVAPPRAAQMGVELLDDVREVCARADFLTVHLPKTPETVGIVGADE
                     LKACRPGVRVVNTARGGIIDESALVEAVRDGTVAGASVDVFDGEPVTEHPLFSLPNVV
                     VTPHLGASTAEAQDKAGMAIAEQVLLALRGDFVPFAVNIDIGPDIPEPVRPYVQLAER
                     LGRVAVALSGSGVESLRFEYHGGLGEQDTRALTLSGLKGAFSAVVHEPVTFVNAPLLA
                     KERGIVIEESKSSQSLDYVNLIEVRASSEDETVSVAGVLVGKRDTERLVRVYGYDLDM
                     AFAPVMAFFRYVDRPGVVGIVGTLLGEASINIANMQVGRQTVGGEALMGLAVDSPIPQ
                     DVLE"
BASE COUNT         1125 a         2554 c         2508 g         1075 t
ORIGIN      
        1 gcatggagga agggtcgatg cgcgtcgacg cgaacgtctc ggtccggcgt cgcggcgaga
       61 aggagttcgg ggcgaaggtc gaggtcaaga acatgaactc gatccgctcg gtgggccggg
      121 ccctcgagta cgaggagcga cgccagcgcg acgctctcga cgccggcgag tcgctgatcc
      181 aggagacgcg gcacttcgac gagaagtccg gcacgacgtc gtcgctgcgg accaaggagt
      241 tcgcggtcga ctacggctac ttccccgagc ccgacctcgt gccgttcgag cccggcgacg
      301 aatgggtcgg caaggtccgc gccgagctcc ccgagctccc ggccgcccgc cgggagcggt
      361 tcgcgcagga gtacgggctc gccgagggcg acatcgcgat cctcacggcg acgaaggcga
      421 ccgccgactg gttcgaggaa gccgcacgcg cctacggcgg ccaggccaag aagatcgtga
      481 actggatcat cgccgacctc ttcgggctcc tgaacgaggc cgggatcgag ctcggcgagt
      541 cgaagatcgc cccgcgccag ctcgcggggc tcgtcgagct cgtcgactcg ggcaaggtct
      601 cggggaagca ggcgaagctc gtcctcgccg gcatgttcga gaccggttcg gacgccgacg
      661 ccgtggcgcg cgagaagggc ctcgagcagg tgtcggacac cggcgcgatc gaagccgccg
      721 tcgacgaggt catcgccgag aacgccgacg cggccgacaa ggtgcggggc ggccagacga
      781 acacgatcgg cttcctcgtc gggcaggtca tgaagaagac ccgcggccag gcgaaccccg
      841 gcatggtcaa cgacctcctg agaaagaagc tcacctcctg accagaccgc gggcggcctc
      901 ggccggacgc cgggacaagg tcgtcgccct ggagatgacc gaggtctgct cggggccccc
      961 ggagtcgatc tgggacctga tcactgactg ggagcaccag gacgactgga tgctcgaggc
     1021 gagcgacttc gtcgtggtgt cggacgtccg ggaaggcgtc ggcgtcgagg cggaggccac
     1081 gatccggatc ggcgggataa cgacccgcga ccgggtccgg gtgggtgaat gggacccgcc
     1141 ccggcggctc gtgatcgagc acctcggctg ggtgaccggg cgggggatca tcgagctcac
     1201 gccggtggcc ggccgccgca cccacgtgtg gtggcgggag gagctgagcc cgcccctggg
     1261 ggtcctcgga gcgatcggga tgacggcgtt caagcccttg atggcccggg tgttccgcag
     1321 agacctgcgg atcctcgccg ggctcgcccg cgctgccgcc cgctagatcc cccgagaccg
     1381 ccgcgggcct cgacgcagga gcgcaggcgc tggcgtcgta ttgggtagcc agcagatccg
     1441 ccgacgcggc gcatccatac aaggaggaaa acggtgaggg gattccgtgt actcggggcg
     1501 atgatggccc tggcactcac ctccgctatc ccggcaggcg ccgcggagca ggcggagccg
     1561 gtgaagtccg acaacgtgac tcacgtgttc aacgaccagt acttctcggg gaccgagctc
     1621 gcggcctccg gcaagtacgt ctacacgggc cagatggacg gggacgagtc cgtcgatcgc
     1681 ggctcggcgc ccgaagaggg cggcatccgc atctacgacg tctccggcaa ggtgcccaag
     1741 aaggtcgggt tcctgcactg cccgggcaac gacaacgacg tcgaggtcgt caagccgggc
     1801 ctcatcgtcg tcagcttcgc gacgaacaag tgcgccccgc aggccggcaa cggcctcatg
     1861 acggtcgacg tcaagaaccc ccgcaagccc aagatcctcg gcgccgtcaa caccggcaag
     1921 aaccacaccc acaagccgta cccgggcacg aactacgtct acacggcggg tggcggcctc
     1981 tcgggtggcc ccggtgccgg ccctgcgatc gtggacgtgt ccgaccccaa gagcccgaag
     2041 gtcgcgggca agctctcgac gctgacgacc gactgccacg acatctcgtt ccgcatcagc
     2101 aagggttcga agctgatgtt ctgcgccggc gccatcggca ccggtgaggt ccagatctac
     2161 gacgtgtcgg acccgctggc cccggccctg gtctcccgca tcttcaaccc ggcgatccag
     2221 tactcgcact acgcggtcgc cagctctgac ggccagttcc tcgccatcga cgacgaggcg
     2281 ttcgcggccc acgagtgcgc cagcggcaac agcccgaccg gccgtgtgtg gatctacaac
     2341 atcgagaacc cgcagctccc gctcctcgtc ggcagcttcg ctcctcctcg tggcggcgac
     2401 gacacggttc ccgtcggcca cctgcagggc tgggtcccga cgtggtgcct ctcgcacggc
     2461 ctcgactggc agcccggcac gcacaacgtc gccgtgacgt ggttcaccgg cgggtggagc
     2521 ctgatcaacg tcgacggcca gctgccgacc gagatcgccc actaccaggc tgaggactcg
     2581 gcgacgtact cgacgctgtg gcacaacggc aagctgtaca cgaacgactc gttccgtggc
     2641 ttcgacgcct tcaacctgaa gctgaagtaa gagccgctac acaacgaacg caggtcttgc
     2701 gcccccgggt ctcccggggg cgcagccgcg tcgggggcga acacccctgc tctcgaggtg
     2761 tcagacatgt cagacacctc ttgcgacgca cataatgcgt cgatggacag agccgacgtc
     2821 cagctgggtc gggcgctagc gcagcaggtc cgctatgcgg cccacgcctt cgcggatgtc
     2881 gtcgtctccc agcgcgtagc tcagcctcgc gtagcccggg gccccgaagc cttcgcccgg
     2941 cactatcgcc accttcgccc tctccaggat cacctcggcg aggtgggccg cgctctcgat
     3001 cgtgacgccc ccgacgtcgc ggccgatcca ctccgcgaac gacgggaaga cgtagaacgc
     3061 tccctcgggc tcgggacaga tcacgcccgg gatctcgttc agcatcttgt ggatcgtcac
     3121 gcggcgccgg tcgaacgcct cccgcatctc gtagacggcc tggaggtcgc ccgacaccgc
     3181 cgcgagggct gcggcctgcg cgacgttcga cacgttcgac gtcgcatgcg actggagcga
     3241 cgcggcagcc ttcgtcacgt cggccggggc gatcatccag cccacccgcc agcccgtcat
     3301 cgcatacgtc ttcgcgacgc cgttgacgac cacgcagtgg ttcgcgatct cgggtacctc
     3361 gaccggcatc gacgcgaact tggcgtcgcc gtagacgagg tgctcgtaga tctcgtcggt
     3421 gaggacccag atcccgcgct cgactgccca ctcgccgatc gcacgcactt cctctggcga
     3481 gtacacggcg cccgtcgggg tggagggcga cacgaacagg agcatcttcg tgcgcggcgt
     3541 cagcgcgcgc tcgaggtcgt cgagcgagac cttgaacccc gtcgcctcgg tcgtcgggat
     3601 ctcgaccggc gtcgcgccgg cgagcctgat cacctccggg tagctgaccc agtagggggc
     3661 cgggacgatg acctcgtcgc cctcgtcgag cagcgtcagg aacgcgttga agagcgcgtg
     3721 cttcccgccg ttcgacacga ggacctgctc gggctggacg gcgtacccgg agtcgcgctg
     3781 ggtcttgcgc gcgatcgcgg cccggagctc gggcaggccc ggcgccggcg tgtagcggtg
     3841 gtagacggtg tggcgagcgg cctccgccgc cgcctcgacg atgtgagccg gggtcgggaa
     3901 gtcgggctcg ccggcgccga acccgatgac gttctcgccg gcggccttga gcgccttcgc
     3961 cttggcatcg agagcgagcg tcgacgactc ggttatcgcc ccgacgcgtg tgctgatgcg
     4021 ctcgatgatg ctcgcctcgt tccgtccagt aggtttcgcg cgaccctacc aacgcctacc
     4081 gtttggaggc cgcgtccttg ccgagcgaca tcctgatccc accggcgctg cgtccgtccg
     4141 acggccgctt cggctccggc ccttcgaagg tgcgacccga agccgtcgcg gcactggccg
     4201 cggccgcgcc cggctacctg ggcacgagcc accggcagaa gggcgtcaag tccgtcgtcg
     4261 ggcgcttgcg cgaaggtctc gcgaccttgt tcggcctgcc caacggatac gaggtcgtgc
     4321 tcggcaacgg tggtgcgacc gcgttctggg acgcgctcgc gttcaacctc gtcgaggggc
     4381 gcagtcgtca ctacgtgttc ggcgagttct ccgcgaagtg cgcggccgcg gtccgagcgg
     4441 cgcctcacct gcacgagccg gaggtattgg aagcggagcc tggctcccat cccgagcccg
     4501 ccccctcgac tgccgccgac ctctatgccc tcacgcacaa cgagacttcg accggcgtcg
     4561 cgatgcccgt ccggcgcgtc gccccgcagg gattgacggt cgtcgacgcg acgtcggccg
     4621 caggcggcct tccggtcgac ccggcgcagt tcgacgtcta ctacttcgcg ccgcagaagt
     4681 gcttcgcctc cgacggggga ctgtggatcg ggctgttctc gcccgcggcc ctcgagcgga
     4741 tcgagtccgt ggcgaagtcc ggccgctacg tccccgcgtc gctcgacctg gccattgcgg
     4801 tcgagaactc gcgcaaggac cagacgtaca acaccccggc cctcgcgacg ctcttcttga
     4861 tggccgagca gctcgactgg atgctcgcca acgggggcct cgagtggacc gtcggaaggt
     4921 gcgcgcgctc ggcggacctc ctgtacgcgt gggcggaggc gagcgacgtc gcggccccct
     4981 tcgtgcgcga cccggcacag cgcagcgcgg tcgtcggcac gatcgacttc gcagacggtg
     5041 tcgacgcgaa ggcgatcgcg agcgcgctcc gcgcgaacgg gatcgtcgac accgagccgt
     5101 accgcaagct cggccgcaac cagctacggg tcgccatgta tccggcgatc gagcccgacg
     5161 acatcgaggc gctcacgcgc tgcatcgact acgtggtggg ggagctcgcg tgaaggcacg
     5221 cccggcggcc cccgacgacg ctccggcgat agcgcgcgtc cacgtggcgt cgtggaacgc
     5281 ggcgtaccgg ggcgtcatct cggacgaagc gctcgacgag ctgaccgagg agcgactcgc
     5341 gcccgaatgg cgccgcgaga tcgagaccgg cgagtccgaa gtggccgtcg tggaagtcga
     5401 cgccgaggtc gccggctact cgcgctacgg gccgtcgcgc gacgacgacc tcgacccggc
     5461 tacgactgcc gagatctacg gcttctacct ccatcccgac acgtggggca ggggagccgg
     5521 ccgggtcctg atgacccacg tcaaggacga tctcggaacc cgcggttttc gcgacgccgt
     5581 cctgtgggtc gtccaggtga acgaccgggc ccaggccttc taccgggcgg tgggcttcgc
     5641 ccccgacggc cgcgacgaca agctctgcat aggggctccc gagtaccgct acggcgggga
     5701 gctgccgtcc tagaccgact tgtacgatgc ggcccgtgaa ggtcctcgtc tccgagaagc
     5761 tctccgccgc aggtgtcgaa cggctccgcg agcacgtcca ggtcgacgtc cggcccgggc
     5821 tgtcgcccga cgagctcctc ggcgtcatcc ccgactacga cgccttgatc gtccggtcgg
     5881 gcacgaaggt caccgcggag gtgctcgagg ccggcaagag cctcaaggtc gtcgggcgcg
     5941 ccgggatcgg gctcgacaac gtcgacgtcg acgcggcgac ccgccacggc atcctcgtcg
     6001 tgaacgcccc tcagtcgaat gtcctttcgg cggccgagca cacggtcgcg ctgatgctcg
     6061 cccaggcccg caacatccca caggcccact cggcgctcgt ctccggctcg tgggagcgcg
     6121 agcggtggca gggggtcgag ctccacggca agacgctcgg gatcctcggc ctcggcaggg
     6181 tcgggaccct cgtcgcgcag cgcgcgagcg ccttcggcat gcggctcgtc gcctacgacc
     6241 cgtacgtcgc tccgccgcgt gcggcccaga tgggcgtcga gctcctcgac gacgtccggg
     6301 aggtctgcgc ccgcgccgac ttcctgacgg tccacctccc gaagaccccc gagacggtcg
     6361 ggatcgtggg ggccgacgag ctgaaggcgt gccggcccgg cgtcagggtc gtgaacacgg
     6421 cgcgcggcgg gatcatcgac gagtccgcgc tcgtggaagc ggttcgcgac ggcacggtcg
     6481 cgggggcgtc ggtcgacgtc ttcgacggcg agccggtcac cgagcacccg ctgttctccc
     6541 tcccgaacgt cgtcgtgacg ccacacctcg gcgcgtcgac ggcggaggcc caggacaagg
     6601 ccggcatggc aatagccgag caggtcctgc tcgcgctgcg cggcgacttc gtcccgttcg
     6661 ccgtcaacat cgacatcggt cccgacatcc ccgagcccgt gcgtccctac gtccagctcg
     6721 cggagcggct gggccgcgtc gcggtcgcgc tctcgggctc gggcgtcgag tcgctgcgtt
     6781 tcgagtacca cgggggcctg ggcgagcagg acacgcgggc gctcacgctg tcgggtctca
     6841 aaggtgcgtt ctcggcagtc gtgcacgagc cggtgacgtt cgtcaacgcc cctctgctcg
     6901 cgaaggagcg ggggatcgtg atcgaggagt cgaagtcgtc gcagtcgctc gactacgtca
     6961 acctgatcga ggtgcgggcg tcgtccgagg acgagacggt ctcggtcgcc ggcgtcctcg
     7021 tcggcaaacg cgacaccgag cggctcgtgc gcgtctacgg ttacgacctc gacatggcgt
     7081 tcgccccggt gatggcgttc ttccgctacg tcgaccgccc cggcgtcgtg ggcatcgtcg
     7141 gcacgctcct gggcgaggcg tcgatcaaca tcgcgaacat gcaggtcgga cggcagaccg
     7201 tcgggggcga ggccctcatg ggtctggcgg tcgactcgcc catccctcag gatgtcctcg
     7261 ag
//
LOCUS       DASQDO010000364         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_956735, whole genome shotgun sequence.
ACCESSION   DASQDO010000364 DASQDO010000000
VERSION     DASQDO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956735"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VEV43_10230"
     CDS             complement(<1..159)
                     /locus_tag="VEV43_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYP23941.1"
                     /translation="MAPSDEPTASAMQADAVAAALAQAHRSEWAFVLSATVRVAGDLD
                     VAEECAQEA"
     gene            complement(159..515)
                     /locus_tag="VEV43_10235"
     CDS             complement(159..515)
                     /locus_tag="VEV43_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYP23942.1"
                     /translation="MAKYAILIYDDPAVYANMSPEAWGALVDAHSTFVKQVFELGGSL
                     AGGEALAPTTTATTIKGAGTVTDGPFVETKEALGGFYVVEARDLDHAVEIAKLCPAPF
                     GGVEVRPVVDPTTNPF"
     gene            complement(847..1545)
                     /locus_tag="VEV43_10240"
     CDS             complement(847..1545)
                     /locus_tag="VEV43_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23943.1"
                     /translation="MPPILVLRGTRNLKARYETFKARCHDVVEVGSPNGLFEDVLIGR
                     LDGHPIAYAAVYGPAMASEIAHVFGVMGTGVVIQTGLCGALGDGIGPGDLIVASNAGC
                     GEGGVACYTPDVRSVDASPELVDLACAVDVGGVPRHAGPIWTTAALLAEGEAEIAEWH
                     RDGWIAMDMETATTYGVAAWAGMRRVSVLSVFDNPRDGGHIALAEADKAEARAAGEAA
                     AQRVVEELVRNLAS"
BASE COUNT          268 a          624 c          574 g          270 t
ORIGIN      
        1 cgcctcctgc gcgcactcct ccgcgacgtc caggtcgccc gcgacgcgca ccgtggcgga
       61 cagcacgaag gcccactcgg agcggtgggc ctgcgccagc gcggcagcga ccgcgtccgc
      121 ctgcatggcg gacgcggtcg gttcgtcgct gggagcgatc agaacgggtt cgtcgtcggg
      181 tccacgaccg ggcgaacctc gacgccgccg aacggggccg ggcacagctt ggcgatctcg
      241 acggcgtggt cgaggtcgcg cgcctcgacg acgtagaagc cgcccaaggc ctccttggtc
      301 tcgacgaacg ggccgtccgt caccgtgccg gcacccttga tcgtggtggc cgtcgtcgtg
      361 ggcgcgagcg cctccccgcc cgcgagggac ccaccgagct cgaagacctg cttgacgaac
      421 gtgctgtgcg cgtcgaccag cgccccccac gcctcgggcg acatgttcgc gtagacggcc
      481 gggtcgtcgt agatgaggat cgcgtacttg gccatggagc tgcctcctgc actgggtcgc
      541 gggccggcct gtccggctcc gtcataagag acgacgaaca ccctgccgcc gaatcgacag
      601 ccaggcctgg atcttcttca agatttttcc ggcgccgccg ccgtgcgtgc cggagtccgc
      661 tttgcgtgct gaagtgcact ccagcggccc gacctggcgc tccgtctcgc cgacacctgg
      721 gccggattcc gactcagggt aagtccgagg acaaccccgg tcgtcttcga ccacgtcacc
      781 gctcctaccg tgatcagctc ggcccgctct ccaccggtca cgatcatctg gacggtaacc
      841 acggcgtcat gatgctaggt ttcggacgag ctcctcgacg acgcgctggg cagccgcctc
      901 gcccgctgcc cgagcctccg ccttatcggc ctcggccaag gcgatgtggc caccgtcccg
      961 cgggttgtcg aagacgctca gcacggagac gcggcgcatc ccggcccaag cggcgacacc
     1021 gtaggtggtg gccgtctcca tgtccatggc gatccaccca tcgcggtgcc attcggcgat
     1081 ctcggcctcc ccctccgcga ggagcgccgc ggtggtccaa attggtccgg cgtgacgcgg
     1141 cacaccgccg acgtcgactg cgcacgccag gtccacgagc tccggggagg cgtcgaccga
     1201 ccggacgtcg ggtgtgtagc acgcgacacc gccctctccg cagccagcgt tggaggcgac
     1261 gatcaggtcg ccgggtccta tgccgtcgcc gagcgcgccg cagaggccgg tctggatgac
     1321 gaccccggtg cccatcacgc cgaagacgtg ggcgatctcc gacgccatcg ccggcccgta
     1381 cacggcggcg tacgcgatcg gatggccgtc gagccgtccg atcagcacgt cttcgaacag
     1441 cccgttcgga gagccgacct cgaccacgtc gtggcagcgg gctttgaagg tctcgtagcg
     1501 cgccttgagg ttgcgtgtcc cccggaggac gaggatcggg ggcacctgct cctcccgcac
     1561 cccgaggaac cctagccagt ccgccttcgt cagctcccga agcactgtgc ctcccggttg
     1621 acctatgagt gtcgacgcaa gggatcttag caggggagct tacaagaacg gggggagcct
     1681 tcttagagac tgaatcaaaa ccggccttcg atccggcgga ggcagatgag cgagca
//
LOCUS       DASQDO010000365         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_406612, whole genome shotgun sequence.
ACCESSION   DASQDO010000365 DASQDO010000000
VERSION     DASQDO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406612"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VEV43_10245"
     CDS             complement(<1..815)
                     /locus_tag="VEV43_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HYP23944.1"
                     /translation="MAKAKFERTKPHVNIGTMGHIDHGKTSLTAAITKVLSENNPNVS
                     FTAFDQIDKAPEEKERGITIAIAHVEYETDNRHYAHVDMPGHADYIKNMITGAAQVDG
                     AILVVSAADGPMPQTREHVLLARQVNVPYIVVALNKVDMVDDPELLDLVELEVRELLT
                     EYEFPGDDTPVTRVSALKALDGDAEAAKGILELMAAADEYVPEPERDTAKPFLMPIED
                     VFSITGRVERGIIKVGEEVEIIGFRDTTKTVVTGVEMFRKLLDEGRAGDNIGAL"
     gene            complement(836..>1817)
                     /gene="fusA"
                     /locus_tag="VEV43_10250"
     CDS             complement(836..>1817)
                     /gene="fusA"
                     /locus_tag="VEV43_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572746.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYP23945.1"
                     /translation="GDIVAIVGLKQTLTGDTLCDPSDPVLLEEMVFPTPVIDVAIEPK
                     SKPDSDKLGNALQRLSEEDPTFRVHTDDETGQTIISGMGELHLEVLVDRLIREFKVGA
                     NVGKPQVAYRETVQDPVEKAEVRYVKQTGGKGQYGHVILAIEPTGPGGGYEFINKISG
                     GRIPTEYIPAVDQGVQEAMASGVLAGYPVVDVRVTLVDGSYHDVDSSEMAFKITGSMA
                     FKEAAKKAKPVLLEPVFDVEVVTPDEYMGDVIGDLSSRRGRIESMDQRGSDRIVKAQV
                     PLAEMFGYATELRSRTQGRATYTMQFDSYKETPKNIADEVVATVRGEPTS"
BASE COUNT          257 a          600 c          596 g          364 t
ORIGIN      
        1 agcgcgccga tgttgtcacc cgcgcggcct tcgtccagca gcttgcggaa catttccacg
       61 ccggtcacga cggtcttcgt cgtgtcgcgg aagccgatga tctcgacttc ctcgccgacc
      121 ttgatgatgc cgcgctcgac acgacccgtg atcgagaaga cgtcctcgat cggcatcagg
      181 aacggcttgg cggtgtcgcg ctcgggctcc ggtacgtact cgtcggcggc agccatcagc
      241 tcgaggatgc ccttggccgc ctcggcgtcg ccgtcgagag ccttcaaagc cgagacacgg
      301 gtgaccggcg tgtcgtcacc cgggaactcg tactcggtga gcagctcgcg cacctcgagc
      361 tcgacgaggt cgaggagctc ggggtcgtcg accatgtcga ccttgttgag cgccaccacg
      421 atgtagggca cgttcacctg acgggcgagc agcacgtgct cacgcgtctg gggcatcggg
      481 ccgtccgccg cggacaccac gaggatggcg ccgtccacct gggcggcccc cgtgatcatg
      541 ttcttgatgt agtcggcgtg gccgggcatg tcgacgtgcg cgtagtgacg gttgtccgtc
      601 tcgtactcga cgtgagcgat ggcgatcgtg atgcctcgct ccttctcctc cggcgccttg
      661 tcgatctggt cgaacgcggt gaaggacacg ttggggttgt tctcgctcag taccttcgtg
      721 atagcggccg tgagcgaggt cttcccatgg tcgatgtgac ccatggtccc gatgttcacg
      781 tgtggcttgg tgcgctcgaa cttggccttg gccatccctc ttcctttctt cctacctagc
      841 tcgtcggctc gccacgcacg gtggcaacta cttcgtcggc aatgttcttg ggcgtttcct
      901 tgtacgagtc gaactgcatg gtgtaggtcg cgcggccctg ggtgcggctg cggagctcgg
      961 tcgcgtagcc gaacatctcg gcgagcggga cctgggcctt cacgatgcgg tcgctgccgc
     1021 gctggtccat cgactcgatg cggccgcgac ggctcgagag gtcgccgatg acgtcgccca
     1081 tgtactcgtc cggggtgacg acctcgacgt cgaagaccgg ctcgaggagc acgggcttcg
     1141 ccttcttggc cgcctccttg aacgccatcg agccggtgat cttgaacgcc atctcggagg
     1201 agtccacgtc gtggtacgag ccgtcgacga gcgtcacgcg cacgtcgacg accgggtagc
     1261 cggcgaggac ccccgacgcc atggcctcct gtaccccctg gtccaccgcc gggatgtact
     1321 cggtcgggat gcggcctccg gagatcttgt tgatgaactc gtagccaccc ccgggccccg
     1381 tcggctcgat ggcgaggatg acgtgtccgt actggccctt accgccggtc tgcttgacgt
     1441 agcggacctc ggccttctcg accgggtcct ggacggtctc gcgatacgcg acctgtggct
     1501 tgccgacgtt ggcgccgacc ttgaactcgc ggatcagacg gtcgaccagc acctcgaggt
     1561 gcaactcgcc catccccgag atgatcgtct ggccggtctc gtcgtcggtg tgcacccgga
     1621 acgtcgggtc ctcctccgac agacgctgga gcgcgttgcc gagcttgtcc gagtccggct
     1681 tcgacttcgg ctcgatcgcg acgtcgatga ccggcgtcgg gaagaccatc tcctcgagca
     1741 gcacggggtc gctcgggtcg cacagcgtgt cgccggtgag ggtctgcttg aggccgacga
     1801 tcgccacgat gtcgccc
//
LOCUS       DASQDO010000366         5885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_161838, whole genome shotgun sequence.
ACCESSION   DASQDO010000366 DASQDO010000000
VERSION     DASQDO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161838"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VEV43_10255"
     CDS             complement(<1..520)
                     /locus_tag="VEV43_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23946.1"
                     /translation="MPSLTLTPETHLLLLTTVGLASVSPAALTERLSDVVPAPRGARR
                     ETNYVVVLAAVAGAGVLLADPVLVVDPPLWRAAVSILAGLPAGMLAFRADQAVLRSYR
                     RRRQLEALGRSGRLRQPSQVGTPVRGGPHVPSGWQLTAIGILEEVVFRGVLFSLALAS
                     GSPWVAAGLVVVV"
     gene            complement(495..1841)
                     /locus_tag="VEV43_10260"
     CDS             complement(495..1841)
                     /locus_tag="VEV43_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HYP23947.1"
                     /translation="MKLASAPLHYGDQRYTDGLTRRMLSPLCGLNTSIQSFLRGSADP
                     RIVVVGAEITGVHEILGRPPPTHSYHIGGGGVFPEEALIRTLGESLERYAQLMGGIRH
                     RGGLRFTSYDSLLAKGERVVDPEYLTFFEPHQLSQPGFLFEAFRRDAPVAWLHVRSLT
                     GGETTWAPAQILTLGYNVHQAAGEPWIVSAVTTGTAAHTDPTKALRNCILELIQLDTV
                     MGHWYTDRVARRIELDRRTAPISRILGKFFQRGLDAAFYTIENADLPGHHIACKITDA
                     DGIPSIAIGLGTDTNLVDAMYKAMLEGVGVVKLARLLAIQMQTRRGGAIEIDPTSLYD
                     LDNNVLYYALPENRHLLERRFGDDPAVHASDLDPDWGPDPAADVRGLVQAFADTGKDL
                     FFLDLTTPDIAALGFYAFRAWSPQTLALTLPSAPALAHARYREYGGCTHAEPHPYP"
     gene            complement(1838..2872)
                     /locus_tag="VEV43_10265"
     CDS             complement(1838..2872)
                     /locus_tag="VEV43_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HYP23948.1"
                     /translation="MRIRRDVSLIAHSPNDVELRHGIWNPISYTLRDEDKTEKLFAIL
                     SDLRSGVRPKEIARRHDSPRSDVEGLIDRLVELGVMESGPATAIDYHLEKVTPLASGV
                     DGDVRRVRMLGDSALAARVGALVGDTVGADSVVQVPAGDPGWARLCELNHEWLTDAVP
                     FAAAIETFEPWKDDLVVLAQDVVDPLVAQALNRVAREVGFSWLYGAMDGPFLFVGPLT
                     VPGEGGCFECFERRVTMNLRAAQSYQEYKSALADGRVSGGTMEVAPVIEHMLAAHLAF
                     EVTNFALTGRTFTVSKVLAIYLPTMEISFNEVLKLPGCAGCDSAPLRDEKELYFDLQA
                     MLGVPGTNTP"
     gene            complement(2885..3844)
                     /locus_tag="VEV43_10270"
     CDS             complement(2885..3844)
                     /locus_tag="VEV43_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HYP23949.1"
                     /translation="MPKMRDRGDSVLDVGVTEDEPDRDLLLAAIRENSFKPVVAMNQT
                     TGLQLAQLRHATGVASGTVGIAEEFLAGSRLAPGDFEVPGSILKYFDDSSQVMLAQGG
                     QRAVPAEGSVELPPPVRLPLDVGTALRKRRSRREYTGDPITLPQLSTLLWAAAGVTGK
                     VLSELTSGSVATISCRTAASGGGLFPIDVYVVGLRVTGLDPDVYLYSPPRHGLLPCDA
                     DVGEVLEGLHLPDDQISGRELAAAFLFVGSPWKSMRKYGARGVRYMFIEAGAMSQGLH
                     LARTALELGGVDYGGFYDSATNRALGLDGRYETVVHAVVCGVE"
     gene            complement(3936..4457)
                     /locus_tag="VEV43_10275"
     CDS             complement(3936..4457)
                     /locus_tag="VEV43_10275"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP23950.1"
                     /translation="MSIGADDPENLANFYRDAFGDELWGRNLTKYDVSFSTWIAAGVK
                     LSIGSRHHPDDRVMFHIIVDSLEETAAAVERAGGSMTAGPMDIPVAEDALDAFSDNYG
                     KYVKGGDKADVTSSMGQVAIFDDPEGNPFGAMQLEPWATKLFKGSVVGEFEIGESKVG
                     RKSSDAFHAANPA"
     gene            complement(4496..4690)
                     /locus_tag="VEV43_10280"
     CDS             complement(4496..4690)
                     /locus_tag="VEV43_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23951.1"
                     /translation="MDIRVRSLSEHRTATGQDVMLGGPQSEFVSLAPGGSTSCFWSSS
                     CTTCCGVVLVDVTDEIGGPR"
     gene            5469..>5885
                     /locus_tag="VEV43_10285"
     CDS             5469..>5885
                     /locus_tag="VEV43_10285"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HYP23952.1"
                     /translation="MVETHLAMQAQDFGPAKWSIGQRLSGSTDVEVERAFTRGSILRT
                     HVLRPTWHFVARRDLRWLLSLSGPRVQKGLQPRYRQLGLDGRTRSRAETVIARALLDG
                     DLTRREVNDVLRRSGIDTEGQRLPHLLIHCELESVIC"
BASE COUNT          940 a         2010 c         2007 g          928 t
ORIGIN      
        1 tcacgacgac caccagcccc gcggctaccc atgggctccc gctcgcgaga gcgagggaga
       61 agagcacccc tcgaaagacg acttcctcga ggatcccgat cgccgtcagc tgccagccgg
      121 acggcacgtg cggcccccca cggacgggcg tgcccacctg ggacggctgc cgcagccggc
      181 cgctcctgcc gagcgcttcc agctgccggc ggcggcggta ggacctgagc acggcctgat
      241 cggcgcggaa ggccagcatc cccgccggca acccggcgag gatcgacacc gcggcgcgcc
      301 acaggggcgg gtcgacgacc aatacgggat cggcgaggag gacgcccgcg ccggcgaccg
      361 ccgcgaggac gacgacgtag ttggtctccc tgcgagcgcc gcggggcgcc ggcacgacgt
      421 ccgacaggcg ctcggtcagc gctgccggac tgacgctcgc caacccgacg gtcgtcagca
      481 gcagtagatg cgtctcaggg gtaagggtga ggctcggcat gggtgcaccc tccgtactct
      541 cggtaccggg cgtgcgcgag ggcgggggcg ctgggcaacg tgagcgcgag tgtctgcggc
      601 gaccacgccc ggaacgcata gaaccccaac gccgcgatgt cgggcgtcgt caggtccagg
      661 aagaagaggt ccttgcccgt gtcggcgaaa gcctggacga gcccccgcac gtcggcggcg
      721 gggtccggcc cccagtccgg gtccaagtcg ctcgcgtgga ccgcggggtc gtcgccgaag
      781 cgccgctcca gcagatgccg gttctccggg agcgcgtagt agagcacgtt gttgtcgagg
      841 tcgtagaggg acgtcggatc gatctctatc gcgccgccgc ggcgggtctg catctggatc
      901 gccaggagac gcgcgagctt cacgacaccg accccttcga gcatcgcctt gtacatggcg
      961 tccacgaggt tcgtgtcggt cccgaggccg atcgctatcg aggggatgcc gtcggcgtcg
     1021 gtgatcttgc acgcgatgtg atgcccgggg aggtccgcgt tctcgatcgt gtagaacgcg
     1081 gcgtccagcc ctcgctggaa gaacttgccg aggatgcggg agatcggggc ggtccggcgg
     1141 tccagctcga tgcggcgggc tacgcggtcg gtgtaccagt ggcccatgac ggtgtcgagc
     1201 tggatcagct cgaggatgca gttccgcagt gccttcgtcg gatcggtgtg cgcagcggtg
     1261 ccggtggtca ccgccgacac gatccacggt tcccctgcgg cctggtggac gttgtagccg
     1321 agcgtcagga tctgcgcggg ggcccaggtc gtttcgccgc cggtaagcga cctcacgtgc
     1381 agccacgcca cgggcgcgtc ccggcggaag gcctcgaaca ggaacccggg ctgcgacagc
     1441 tggtgcggct cgaagaacgt gaggtactcc gggtcgacga cccgttcgcc ctttgccaga
     1501 agcgagtcgt acgacgtgaa gcgcaacccg ccccggtgac ggatgccccc catcagctgg
     1561 gcgtacctct cgagggactc gcccaaggtc cggatgaggg cctcttcggg gaagaccccg
     1621 ccaccaccga tgtgatacga gtgcgtcggc ggcggacgtc cgaggatctc gtgcacgccg
     1681 gttatctcgg cgccgaccac gacgatcctg gggtcggcgc tcccgcgaag gaacgactgg
     1741 atggaggtgt tcaatccgca cagcgggctg agcatgcgcc gcgtgagccc gtcggtgtat
     1801 ctctgatcgc cgtagtgcag cggtgccgag gcgagcttca cggcgtgttc gttccgggca
     1861 cgccgagcat ggcctggaga tcgaagtaca gctccttctc gtcgcgcagc ggcgcggagt
     1921 cgcatccggc gcacccgggc agcttcaaca cctcgttgaa cgagatctcc atcgtgggca
     1981 ggtagatcgc caggaccttc gacaccgtga acgtccggcc cgtgagggcg aagttggtga
     2041 cctcgaacgc gaggtgggcc gcgagcatgt gctcgatcac cggcgcgacc tccatggtcc
     2101 ccccgctgac ccggccgtcg gccagcgcgc tcttgtactc ctggtaggac tgagcggcgc
     2161 gcaggttcat cgtgacgcgt cgctcgaagc actcgaaaca accgccctcg cccggcacgg
     2221 tcaacgggcc cacgaacagg aacgggccgt ccatcgcccc gtagagccac gagaacccga
     2281 cctcgcgcgc gacgcggttc agcgcctggg ccacgagggg gtcgacgacg tcctgcgcga
     2341 ggaccacgag gtcgtccttc cacggctcga aggtctcgat ggcggccgcg aacgggacgg
     2401 cgtcggtcag ccattcgtga ttcaactcac acagccgggc ccacccgggg tcgccggcgg
     2461 gcacctggac gacggaatcc gcgcccacgg tgtctccgac tagagcgcct acccgcgccg
     2521 cgagggccga gtccccgagc atccgcaccc ggcggacgtc cccgtcgacg ccggacgcga
     2581 gcggcgtcac cttctcgagg tggtagtcga tcgccgtcgc gggacccgac tccatgaccc
     2641 cgagctcgac gagccggtcg atcagccctt cgacgtcgga gcgcggcgag tcgtgccgcc
     2701 gtgcgatctc cttcggacgc acgccactgc ggaggtccga gaggatcgcg aagagcttct
     2761 cggtcttgtc ctcgtcgcgg agggtgtaag agatcgggtt ccagatgccg tgacgcagct
     2821 cgacgtcgtt cgggctgtgg gcgatcaacg agacgtcccg ccggatcctc atccaggaga
     2881 cctcctattc gacgccgcag acgacggcat ggacgacggt ctcgtagcgg ccgtcgagcc
     2941 cgagcgcgcg attcgtggcg gagtcgtaga aacccccgta gtcgacaccg ccgagctcta
     3001 gtgccgtgcg ggccagatgc agaccctggg acatcgctcc ggcctcgatg aacatgtatc
     3061 gcaccccgcg tgctccgtac ttgcgcatgc tcttccacgg cgaccccacg aacaagaagg
     3121 ccgcggcgag ctcgcggccg gagatctgat cgtcgggaag gtgcagaccc tcgagcacct
     3181 cgccgacgtc cgcgtcgcac ggcagcagcc cgtggcgggg gggcgagtac agatacacgt
     3241 cggggtcgag cccggtgacc cggagaccga cgacgtagac gtcgatcggg aagagacccc
     3301 cgccactcgc ggccgttcgg cacgagatcg tcgcgacgga cccggacgtc agctcgctca
     3361 gcaccttccc ggtcacgccc gcggctgccc agaggagcgt ggacagctga gggagcgtga
     3421 tgggatcacc ggtgtactcg cgccgtgaac gccgcttccg cagagcggtg cccacgtcca
     3481 gcgggaggcg tacgggaggc ggcagctcca cggacccctc tgcgggaacc gcccgctgcc
     3541 caccttgggc gagcatgacc tgtgacgagt cgtcgaagta cttgaggatg gagccgggga
     3601 cctcgaagtc accgggggcg aggcgcgacc ccgcgaggaa ctcctcggcg ataccgaccg
     3661 tgcccgacgc gacgccggtt gcgtgccgca gctgcgcgag ctgaagcccg gtcgtctggt
     3721 tcatcgccac gaccggcttg aagctgttct cgcggatcgc cgcgagcaac aggtcgcggt
     3781 cgggctcgtc ctccgtgact ccgacgtcga ggacggagtc accgcggtcc cgcatcttgg
     3841 gcatgggcgt gtgtggtcct ttcccggcgc gctacgccaa aggggacggg cccgaacgtc
     3901 gcgctcgggc ccgcggacta gctagggatg acggatcagg ccgggttcgc ggcgtggaac
     3961 gcgtcgctcg atttgcggcc gaccttgctc tcgccgatct cgaactcgcc gacgaccgac
     4021 cccttgaaga gcttggtggc ccagggctcg agctgcatgg caccgaacgg gttcccttcg
     4081 ggatcgtcga agatcgccac ctgacccatc gacgacgtga cgtccgcctt gtccccgccc
     4141 ttgacgtact tcccgtagtt gtcgctgaac gcgtcgaggg cgtcctccgc gacggggatg
     4201 tccatcgggc ccgccgtcat ggacccgccg gcgcgctcga cggccgccgc cgtctcttcg
     4261 agcgagtcca cgatgatgtg gaacatgacc ctgtcgtccg ggtggtgtcg cgagccgatc
     4321 gagagcttca cgccggccgc gatccacgtg ctgaagctca cgtcgtactt cgtaaggttg
     4381 cgtccccaga gctcgtcgcc gaacgcatcg cggtagaagt tggcgaggtt ctcggggtcg
     4441 tccgccccga tgctcactat tccgaggtgg ggcccgtctc ctccgtttcc gttcgtcatc
     4501 gcggtccccc gatctcgtcc gtgacgtcga ccagcacgac tccgcagcac gtcgtgcagc
     4561 tactggacca gaagcacgag gtcgacccgc ccggcgcgag cgagacgaac tcgctttggg
     4621 gcccgcccag catcacgtcc tggcctgtgg cagtcctgtg ctccgacagc gatctcaccc
     4681 tgatgtccac cgcatccccc cgtcagcctc cggttctatc gattccgcgt cgtcactcgg
     4741 tgtcgaccac gactacgccg cacgtggtcg tgcaacagct cgaccagaag caggtcgtgc
     4801 aggcacccgg accgaacgag agagacgtcg cgccagggcc gagcaacagg tcttgaccgg
     4861 tggcagtccg gtactcggac aacgtttcga cgcttatgtc catgacaatc cctcccgtgc
     4921 cgcggccgga ttgacttcgc gaagcgcgcg ctggcttgtc agccgttcta tctcgtgaca
     4981 ccatcgactg tcaagcgtgg agttacagcc cggcggcggc tccgtctcgc gggtcgagct
     5041 tccatacgtt ccgggcgcac gggtccgcga cacccacact gcttcattcg tcgcaagctg
     5101 ttgggtgagg ccgttgccga gccgcaggcg ctcggtggat cgtcgcgtgg atcgatcctc
     5161 agcagctctt cgtcgtggag cgccacgaag acaccgccct gagcgcggtc gatcccgtac
     5221 gggctgtcgc ctgggagacg cgcagtgcgg acgacggttc ctgtctccgg atccagcacg
     5281 acgacctcgg cgacgggatg ccggtcttct gcgtcggacg ggcgtcggta gggtctcgac
     5341 gtgaaccctc ggatcgtccg tcaccgggtg gggaaacgtg actcgacgac atgagaccta
     5401 gagacgtcgc gacgacgcga ctgcaccgaa ccggcctcgc cgtcccgatc gacggtggtc
     5461 cggcccgcgt ggtcgagacc catctcgcca tgcaggcaca agatttcgga ccggcgaagt
     5521 ggtccatcgg tcagcgtctg agtgggtcca ccgacgtgga ggtcgagcgg gctttcacga
     5581 gagggtcgat cctgcggact catgtcttgc gtccgacctg gcacttcgtc gcccgacggg
     5641 acctccggtg gttgctctcc ctgagcggtc cgcgggttca aaaagggctg cagccgcgat
     5701 accgccagct cgggctcgac ggcaggacgc gctcccgcgc ggaaaccgtc atcgcccggg
     5761 cgctcctgga cggcgacctc acccgcaggg aagtcaacga cgtcctccgc aggtccggga
     5821 tcgacaccga gggtcagcga ctacctcatc tgctgatcca ttgcgagctg gagtcggtta
     5881 tctgc
//
LOCUS       DASQDO010000367         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_712046, whole genome shotgun sequence.
ACCESSION   DASQDO010000367 DASQDO010000000
VERSION     DASQDO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712046"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /gene="pafA"
                     /locus_tag="VEV43_10290"
     CDS             <1..1272
                     /gene="pafA"
                     /locus_tag="VEV43_10290"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HYP23953.1"
                     /translation="EVARYLFRRVVSWGRSSNVFLENGARLYLDVGSHPEYATPECDS
                     VYDLIVHDKAGERILEGLLKAAEARLREEGISGDIYLFKNNTDSAGNSYGSHENYLVS
                     RYGEFGRLAEVMIPFFVTRQIFAGAGKVLQTARGALFCISQRAEHIWEGVSSATTRSR
                     PIINTRDEPHADAEKYRRLHVIVGDSNMSEWASYLKVGATALLLRMVEEGVQIRDLTL
                     ENPIRAIREISHDTTCKRRVRLANGREASALDMQKEYLEKALRWVERRDDKVLTEIAD
                     MWEHAITSLEHDQDALVEQVDWIAKRRLIESYRDRHKISLAHPKVALMDLAYHDVNRK
                     RGLYYLLERRHKMERICDEADIETATQEPPQTTRAKLRGEFIRAAKRKRRDFTVDWVH
                     LKLNDQAQRTVLCKDPFRSQDERVAKLIASL"
     gene            1273..2346
                     /locus_tag="VEV43_10295"
     CDS             1273..2346
                     /locus_tag="VEV43_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HYP23954.1"
                     /translation="MATFREGVVRRVVEELPGLVRVEVDLSSGESCAADGFPSMLGRV
                     EQGHRVVVNTTGIELGLGTGGRGFLLWDLDAPGPEAAGPGHIVKMRYTPWQTNVLAAE
                     EEASEHYDALVEATSIDGMPVVACGLHSQVPAVAAGIRAELPGARVGYLMTDGAALPL
                     AWSDLVRAARSAGLLDVTATSGHAFGGDLETVNVFSGLVALRRAEAVDVAMVAMGPGV
                     VGTGTALGFTAIEQGQALDAAAALGGRGVACLRVSFADQRPRHRGVSHHTLTALRLAA
                     REPCDVVVPALPETEALLEQLRDLDDRHRVHVVDPAPGMELLRGTGLRLSSMGRSLDE
                     IPETFAAAVAAGTHAAGLVDSVG"
     gene            2346..3302
                     /locus_tag="VEV43_10300"
     CDS             2346..3302
                     /locus_tag="VEV43_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HYP23955.1"
                     /translation="MRRIERLINLIAALLETSQPMTADEIRREIAGYDSDGLDAFRRA
                     FERDKADLRAMGIPIEVRPVEPLSEVEGYVIPKSLYYLPQLDLEPDELAALRLAAESV
                     LGGGEVAESGLMKLSVDAATESWSGPRVVWGADLAAEQPALATIYPALLERRSVAFGY
                     RSGGAAEAREREIDPYGLVHRRGNWYVVGRDRSIDDVRVFKVARIEDAPRMLDHTYEV
                     PDGFRAEDHVAGEAWELGDAPATATVRFDPEVRWWAEQNHPDAPRRELPGGAVDVELQ
                     VSNVDALVAWAIGLGPGVEIVEPGPARAAVLEHLAPFLDGAS"
     gene            3299..4252
                     /locus_tag="VEV43_10305"
     CDS             3299..4252
                     /locus_tag="VEV43_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HYP23956.1"
                     /translation="MNGGRVGRRLQRILLLLPYAIHHPGVSVDELSRKFNVPKRELIG
                     DLELVFLCGLPGYGPGDLIDVSIDDDRVHVRMADYFAAPLRFTPAEALTLYAGAAALV
                     DSPGMEEADALRRALEKLGRALGVHGGDEIDVQLASTPGGHLETLEEALLSRRRVALE
                     YFSATRGELTHRTVDPWGLIAALGRWYLVGWDHLSEDERMFRTDRMKDVKILDAAADV
                     PDDFDPEAYRHAFRERGDEATLTMEISPEVSRWFADYYHPRKVETLADGWQKVELAAT
                     TRRWAATLLLQLGAGARRIEPADVLSEARRLAREIAAPYTA"
     gene            complement(4266..4481)
                     /locus_tag="VEV43_10310"
     CDS             complement(4266..4481)
                     /locus_tag="VEV43_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23957.1"
                     /translation="MRKRALTAVAAAAIGLSAMTGTAEAAEDVEVCRKLNAYSHICVR
                     VLEGESLVGVTYCNGFFCMHRDVIVPR"
     gene            complement(4558..>6381)
                     /locus_tag="VEV43_10315"
     CDS             complement(4558..>6381)
                     /locus_tag="VEV43_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP23958.1"
                     /translation="SSGLPAEVPLGHRVPVGESVAGRVLATGQALRLEEVDADESVNF
                     VPKSRSIASSLVVPLRVHGRSLGVLSLAISAGFPYHEDDLRVAQMFADQAAGLIYRAR
                     LHEQAEQRSSDLMALVESSRGLLGTLDVDDLLQHVLDGGSRLSGSRDGFACLFDENGA
                     ITRGVFRGLDKPAIKNLIEMPEVRDAVLRSDATYVKEGSASVAAVGFRTTRGTRGLVV
                     TVADPLILEGREDLLRAFGQQAASAVSSGELYAEVNGKESELSAIIQTVPHPIVLADA
                     HGRIIAINPAAEAVFGISTMFCAGVPVHGNLGNEDVERLISGTGGAHAEIELGSPPRT
                     YKASVNDVEVPGGPMGRVLIMDDVTAEREMIQKQRDFVAMIGHELRTPLTLVKGFART
                     LIRKVDKDRLTPEDAREGLATIDAKAAQLERLIEDLLYVSKIETREATLKVDTTEVSE
                     LLKRVAQEVITDHPGRRISVEAASSVKWPCDETKVALVLRHLLENALKYSDEDVVVRA
                     SEDGTDLTVEVVDRGAGILSSDIPQIFERFRQIDGSSTREHGGTGVGLYLCAQLVRMH
                     HGRIWVDSTWGKGSNFSFSLPRREPVSEIVHITSSPEARSA"
BASE COUNT          959 a         2229 c         2262 g          931 t
ORIGIN      
        1 gaggtggcgc gctacctctt ccgccgggtc gtgtcgtggg ggcgctcgtc gaacgtcttc
       61 ctcgagaacg gggcccggct ctacctcgac gtcggctcgc accccgagta cgcgacgccc
      121 gagtgcgact ccgtctacga cctcatcgtc cacgacaagg ccggcgagcg catcctcgaa
      181 gggctgctga aggccgccga ggcccggctg cgcgaggagg gcatctccgg cgacatctac
      241 ctgttcaaga acaacacgga ctcggccggg aactcctacg gcagccacga gaactacctc
      301 gtgtcgcgct acggcgagtt cggacgtctc gccgaggtca tgatcccgtt cttcgtgacg
      361 cggcagatct tcgccggcgc cggcaaggtg ctgcagaccg cacggggcgc gttgttctgc
      421 atctcgcaga gggccgagca catctgggag ggcgtgtcgt cggcgacgac gaggtcgcgc
      481 ccgatcatca acactcgcga cgagccccac gcggatgccg agaagtaccg caggctccac
      541 gtgatcgtcg gcgactcgaa catgagcgag tgggcgtcgt acctgaaggt cggtgcgact
      601 gcgctgctgc tgcggatggt cgaagagggc gtgcagatcc gcgacctgac gctggagaac
      661 cccatccggg cgatccgcga gatctcgcac gacacgacct gcaagcggag ggtgcgcctc
      721 gccaacgggc gcgaagcgtc ggcgctcgac atgcagaagg agtacctcga gaaggcgctg
      781 cggtgggtcg agcggcgcga cgacaaggtg ctcaccgaga tagcggacat gtgggagcac
      841 gcgatcacct cgctcgagca cgaccaggac gcgctcgtcg agcaggtcga ctggatcgcg
      901 aagcggaggc tgatcgagtc gtaccgggac cggcacaaga tctcgctcgc gcacccgaag
      961 gtcgcgctga tggacctcgc ctaccacgac gtcaaccgca agcgcggcct ctactacctg
     1021 ctcgaacgca ggcacaagat ggagcggatc tgcgacgagg ccgacatcga gaccgcgacg
     1081 caggagcctc cgcagacgac tcgggcgaag ctgcgcggcg agttcatccg ggccgcgaag
     1141 cgcaagcggc gcgacttcac cgttgactgg gtgcacctga agctgaacga ccaggcgcag
     1201 cggacggtgc tgtgcaagga ccccttccgg tcgcaggacg agagggtcgc caagctcatc
     1261 gcctcgctct agatggcgac ctttcgagag ggcgtcgtac ggcgcgtggt cgaggagctg
     1321 ccggggctgg tccgtgtgga ggtcgacctg tcgtccggcg agtcctgtgc cgccgacggg
     1381 ttcccgtcga tgctcggccg agtcgaacaa ggacaccgag tggtggtcaa cacgacgggg
     1441 atcgagctgg ggctcgggac cgggggccgc gggtttttgc tgtgggacct cgacgccccg
     1501 ggaccggagg ctgccgggcc cggccacatc gtgaagatgc gttacacgcc gtggcagacg
     1561 aacgtcctcg ccgcggagga agaggcgagc gagcactacg acgcgctggt cgaggcgacc
     1621 tcgatcgacg ggatgcccgt ggtcgcgtgc gggttgcact cccaggtccc cgcggtcgcc
     1681 gccggtatcc gcgcggagct tccgggcgcc cgcgtcggct acctgatgac cgacggggcc
     1741 gcgcttccgc tcgcgtggag cgacctcgtc cgggccgcgc gctcggccgg tctcctggac
     1801 gtcaccgcca cgtccggcca tgccttcggc ggcgacctcg agaccgtcaa cgtcttcagc
     1861 ggcctggtcg cgctgcgacg ggccgaggcg gtcgacgtcg cgatggtcgc gatggggccg
     1921 ggcgtggtcg gcaccgggac agcgctcggc ttcacggcga tcgagcaggg tcaggcgctg
     1981 gacgccgccg cggccctcgg gggccgggga gtggcctgcc tgcgcgtctc gttcgccgac
     2041 caacgcccgc ggcaccgcgg cgtcagccac cacacgctga ccgcgctgcg cctcgcggcc
     2101 cgcgagccgt gcgacgtcgt cgtgcccgcg ctcccggaga ccgaggccct gctagaacag
     2161 ctgcgcgacc tcgatgaccg ccaccgcgtc cacgtcgtcg acccggcgcc cgggatggag
     2221 ctgctccggg ggacgggcct gaggctttcg tcgatggggc ggtcgctgga cgagatcccc
     2281 gaaacctttg ccgccgcagt ggcggcgggc acacacgccg ccggcctggt cgatagcgtc
     2341 ggctgatgcg gcgcatcgaa cggctcatca acctcatcgc cgcgctgctc gagacgtcgc
     2401 agccgatgac ggccgacgag atccggcgcg agatcgcggg gtacgactcc gacggcctcg
     2461 acgcgttccg ccgggccttc gagcgcgaca aggccgacct ccgcgcgatg ggcatcccga
     2521 tcgaggtgcg cccggtcgag cccctgagcg aggtcgaggg ctacgtgatc ccgaagtcgc
     2581 tctactacct gccgcagctc gacctcgagc cggacgagct cgcggccctg cggctcgcgg
     2641 ccgagagcgt gctcggcggc ggcgaggtcg cggagagcgg gctgatgaag ctgtcggtcg
     2701 acgccgcgac cgagtcctgg agcggccccc gggtcgtatg gggcgcggac ctcgccgcgg
     2761 agcagccggc gctggccacg atctaccccg cgctgctaga acgccggtcg gtcgcgttcg
     2821 gctaccgcag tgggggagcc gcggaggcac gcgagcggga gatcgacccc tacgggctcg
     2881 tccaccggcg cggcaactgg tacgtggtgg ggcgcgatcg ctcgatcgac gacgtccgag
     2941 tcttcaaggt cgcgcgcatc gaggacgcgc ctcgcatgct cgaccacacc tacgaggtgc
     3001 ccgacgggtt ccgagcggag gaccacgtgg cgggggaggc gtgggagctc ggcgacgccc
     3061 ccgcgaccgc gaccgtgcgc ttcgatcccg aggtccgctg gtgggccgag cagaaccacc
     3121 ccgacgcacc gcggcgtgag cttcccggag gcgccgtcga cgtcgagctt caggtgtcca
     3181 acgtcgacgc tctggtcgcg tgggcgatcg ggctcggccc cggcgtcgag atcgtcgagc
     3241 cgggcccggc ccgggcggcg gtcctcgagc acctcgcgcc gttcctggac ggtgcctcgt
     3301 gaacgggggc cgcgtcggcc gccggctcca acgcatcctg ctgctgctcc cgtacgcgat
     3361 ccatcacccc ggcgtcagcg tcgacgagct ctcgcgcaag ttcaacgtgc ctaagcgcga
     3421 gctgatcggc gacctcgagc tcgtgttcct gtgcggcctc cccggctacg gtcccggcga
     3481 cctcatcgac gtctcgatcg acgacgaccg cgtgcacgtc cgcatggccg actacttcgc
     3541 ggcccccctg cgcttcacgc cggccgaggc cctcacgttg tacgcgggtg cggccgcgct
     3601 cgtggactcg cccgggatgg aggaggccga cgcccttcgc cgggccctcg agaagctggg
     3661 ccgtgccctc ggcgtgcacg gcggcgacga gatcgacgtg cagctcgcct cgacgccggg
     3721 cggccacctg gagacgctcg aggaggccct gctgtcgcgc cggagggtgg cgctcgagta
     3781 cttctcggcg acgcgcggcg agctcaccca caggacggtc gacccgtggg ggctgatcgc
     3841 cgcgctcggg cggtggtacc tggtgggttg ggaccatctc agcgaggacg agcgcatgtt
     3901 ccgcaccgac cgcatgaagg acgtgaagat cctcgacgcg gccgccgacg tgccggacga
     3961 cttcgacccg gaggcgtacc ggcacgcgtt ccgtgagcgc ggcgacgagg cgacattgac
     4021 gatggagatc tcgcccgagg tgtcccgctg gttcgcggac tactaccacc cccgcaaggt
     4081 cgagaccctg gccgacggct ggcagaaggt agagctggcg gcgacgacgc gccgctgggc
     4141 agcgaccctg ctgctgcaat tgggggccgg ggcccggcgg atcgagccag ccgacgtcct
     4201 ctccgaggcg agacggctgg cgcgagagat cgctgcgccc tacacggcct gacgctcggt
     4261 cgcggtcagc gcgggacgat cacgtcgcgg tgcatgcaga agaacccgtt gcagtaggtc
     4321 acgccgacga gcgactcccc ctcgaggacc cggacgcaga tatgggaata ggcgttcaac
     4381 ttccggcaga cctcgacgtc ctcggccgcc tccgcggtcc ccgtcatcgc gctcaggccg
     4441 atcgccgctg ccgccaccgc cgtaagagcc cttttcctca tgtcctgctc cctccgtcgc
     4501 ccagacaatg acgaggaagg attcgggacg gcaggcgccg gttcctcccg gtgagggtca
     4561 ggccgagcgg gcctccgggc tcgacgttat gtgcactatc tcgctcaccg gctcgcggcg
     4621 ggggagcgag aacgagaagt tcgaaccctt gccccacgtc gagtcgaccc agatccgccc
     4681 gtggtgcatc cgcacgagct gggcgcacag gtagagcccg acgcccgtgc ccccgtgctc
     4741 gcgggtcgac gagccgtcga tctgacggaa gcgctcgaag atctggggga tgtcgctcga
     4801 gaggatgccg gcccccctgt cgaccacctc gaccgtcagg tccgtcccgt cctcggaagc
     4861 cctgacgacg acgtcctcgt ccgagtactt cagggcgttc tccagcaggt gccgcagcac
     4921 gagcgcgacc ttcgtctcgt cgcacggcca cttcacggac gaggcggcct ccaccgagat
     4981 gcgacgtccc gggtggtcgg tgatgacctc ctgagcgacc cgcttgagca gctcggagac
     5041 ctcggtcgtg tccaccttca gcgtcgcttc gcgcgtctcg atcttcgaca cgtacaggag
     5101 gtcttcgatc agccgttcga gctgcgcggc cttggcgtcg atcgtcgcga ggccctcgcg
     5161 cgcgtcctcg ggagtcagcc ggtccttgtc gaccttgcgg atgagcgtgc gggcgaagcc
     5221 ctttaccagc gtgagcggcg tgcgtagctc gtggccgatc atcgccacga agtcgcgctg
     5281 cttctggatc atctcgcgct cggccgtcac gtcgtccatg atcagcaccc ggcccatggg
     5341 cccgccgggc acctcgacgt cgttcacgct cgccttgtag gtgcggggcg ggctgcccag
     5401 ctcgatctcc gcgtgcgctc cgccggtgcc cgagatcagc cgctcgacgt cctcgttgcc
     5461 gaggttcccg tggaccggca ctccggcgca gaacatcgtc gagatcccga agacggcttc
     5521 cgccgcgggg ttgatcgcga tgatgcgccc gtgggcgtcc gctaagacga tcgggtgagg
     5581 gacggtctgg atgatggccg agagctccga ctccttgccg ttgacctccg cgtagagctc
     5641 gcccgagctc acggccgaag ccgcctgctg gccgaacgca cgcaggaggt cctcacgccc
     5701 ttcgaggatg agggggtcgg caacggtcac gacgagcccg cgcgtgccgc gggtcgtccg
     5761 gaagccgacc gcggcgaccg acgccgagcc ttccttcacg tacgtcgcgt ccgagcggag
     5821 caccgcgtcg cgcacctcgg gcatctcgat gaggttcttg atcgcgggct tgtccagccc
     5881 ccggaacacc ccgcgcgtga tggccccgtt ctcgtcgaac agacaggcga acccgtcgcg
     5941 tgacccggag agacgcgagc ccccgtccag cacgtgctgc aagaggtcgt cgacgtcgag
     6001 cgtgccgagg aggccccggc tcgactcgac cagcgccatg aggtccgaag agcgctgctc
     6061 cgcctgctcg tggaggcgcg cccggtagat caggcccgcg gcctggtcgg cgaacatctg
     6121 ggcgacccgc aggtcgtcct cgtgatacgg gaagccggcc gagatcgcga ggctgagcac
     6181 gcccagcgag cgcccgtgga cgcgcagcgg gacgacgagg ctcgaagcga tggagcgcga
     6241 cttcggcacg aagttcacgg actcgtctgc gtcgacctcc tccaggcgca gggcctggcc
     6301 cgtcgccagc acgcggccgg cgacgctctc accgacgggt acgcggtggc ctagggggac
     6361 ctcggcgggg agcccgctgg a
//
LOCUS       DASQDO010000368        12240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1140706, whole genome shotgun sequence.
ACCESSION   DASQDO010000368 DASQDO010000000
VERSION     DASQDO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140706"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VEV43_10320"
     CDS             complement(<1..199)
                     /locus_tag="VEV43_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23959.1"
                     /translation="MRKSVSTGLAGVLAASALMIASPAQAQPEVCDSPDPVVAYVCHT
                     VESIPGWVRYYYDEVGQTAQEI"
     gene            complement(214..459)
                     /locus_tag="VEV43_10325"
     CDS             complement(214..459)
                     /locus_tag="VEV43_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23960.1"
                     /translation="MRRKLGVFLATALAGGMIALGPASPASAGPCDEIISTDDPIILF
                     ACRVAGSVPEPGPTVTHYYNLAFDTVHFAYCTVSPNC"
     gene            complement(485..724)
                     /locus_tag="VEV43_10330"
     CDS             complement(485..724)
                     /locus_tag="VEV43_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23961.1"
                     /translation="MRKKMSLVAVGLLAGATMSMTASPAHAQPDPNCAATDPVLAYVC
                     RIINSAPEPGPTINHYYYQVTGAVHTAYCQVSPYC"
     gene            878..1891
                     /locus_tag="VEV43_10335"
     CDS             878..1891
                     /locus_tag="VEV43_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23962.1"
                     /translation="MCASVLVATAVFGGEAAAARPLVCQDHEVTIAGTPGDDRLAGTK
                     GADVIAGLGGNDVVRGVGGNDLICGNAGDDMLRGGRGDDGLDGGRGDDGLRGGKGFDL
                     VTFERAAGGVIVDLTGGRASGDGIDTIESVWWVVGSPHADAVNGGPDMNVVIALGGHD
                     VVAGRGGGDLLLGGPGRDSLAGGKGWDRLLGEGGDDALRGGLGEDTVSYASARDPVSV
                     DLERGRARGEGRDSLSMVENVEGSPQRDVLEGDGGPNEILGAEAGDRIRGRGGNDRIL
                     GLGGDDRLWLGAGDDLGRGGRADDWIDGGLGIDRVYGGAGHDVCRNGEVLGCDEYELP
                     VPP"
     gene            complement(1976..2623)
                     /locus_tag="VEV43_10340"
     CDS             complement(1976..2623)
                     /locus_tag="VEV43_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23963.1"
                     /translation="MMTRGGRLALIVSAVVFVSGACGAPEGTSGRSSPGPSFWSLINR
                     LAVDYEYHDTLEALWGDSDVAVIGHMKNVREGRGLGGKKGQDVTTESVIVTFQVDEVV
                     RGQLNPDSRRIVDLELRRPSLVSVNELDDATPDDRMLLLIYDQAGTLPNVEIDESGAE
                     RDPGKTLYALTTHKALFIEHDGGLTTPLNTTPGPFEESFDARTIDELAQEIRGFE"
     gene            complement(2650..3195)
                     /locus_tag="VEV43_10345"
     CDS             complement(2650..3195)
                     /locus_tag="VEV43_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23964.1"
                     /translation="MRATRLLTVAVLAGVTLTSLLPRAEANYFGPVDGCCEYADNAEH
                     TVYYEIVGNQSYRDAFDHIVGHLDNATVMTTKKVDTKTSATDVRMIADYVGGSGFHGE
                     WDCTALNSAGECETAKLTLNRGSLDGAGQHNKDKVACHEMGHSVGLDHAYQISSCMEQ
                     GLDGPPRFFADHDKTHINNHY"
     gene            complement(3302..5044)
                     /locus_tag="VEV43_10350"
     CDS             complement(3302..5044)
                     /locus_tag="VEV43_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP23965.1"
                     /translation="MREARRRRRSRALPLLVVAVLLGGGGYVLTRSDGEPDREPPAPT
                     TEALKTEDGEPAVDLPTKAYLRDACKLPPKWVKRIHRGWAPGGIRDYDLVIVPKPPNY
                     MGTFVNTSHSAPYDFLQKVPLIFYGKGFVDPTGHVAVDREPTISDLAPTYADLMKFDE
                     WPDRTATSLADLLKETDDAPNLIVTAVIDGGGWNALEEWPDAWPFMKEMISEGLNVEE
                     AIVGSSPSITPATHTNLSTGTFPRYHGVTAIAVRALDGSIVGSFSEVPGNPGPAVMDA
                     RVTMEKNTLADVWDRENGNEPLIGMLAAGNLQLGMIGHGSALQGGDKDVAAIMSRGEW
                     ATNPEFYSLPEYVNTDVPGPETYQEEVDRADGQADGKWRGHDVEPLDGSPAFAPWEND
                     TMFEIMEREGFGKDEMTDLFYLNYKAPDKAGHTWNMLAPEQEDVIKSVDDALRELVGW
                     LDDNVGEEEYVLVVTADHGQTPLGGWAISRSEISGDLGRQFDGIANEKGVIGRTSASS
                     FFSNPDEMEANGVTPEQVSSFLSRYTIGDNIPEGAEIPDGFADRLDERIFDAVFPGRK
                     LPKIVECTNALAEG"
     gene            complement(5041..6744)
                     /locus_tag="VEV43_10355"
     CDS             complement(5041..6744)
                     /locus_tag="VEV43_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP23966.1"
                     /translation="MRKAVAAVVALLLAAACTNSDAEEPRAEATPRPSQAPNQGRAPI
                     ASPEPVTQAPDGWFEAACNLPLEQLKRVRRGYVPGRSPELLVVPREPNYFGSFASYTH
                     SGPWDYVQKIPLVFYGPGFIKEQGEITLDRETTIADLAPTLAELLDTPFPGGRPGNPV
                     TEALLPEEERNGIPKLIVTIVWDGGGWNVLEAWPDSWPYLKKMMEKGTSIGNTEVGSS
                     PSVTPAVHTNLGTGTFPKQHGIVDIPVREGVELFGAYDGFTPRFLQVPTLADLYDRRT
                     GNEAKIGMFAYKPWHLGMIGHGSYVPGGDKDIAFISEKFAGELKTNPEYYSLPPYLED
                     VEGFEEDVRKVDLDDGKIDDLWMGHEVLNDPNKLRHTPVWALYQTRLLKAVLEGEDFG
                     GDDVADLFFINYKQIDDVGHDWNMLNPETREILVYSDKELRKIVQFLDEQVGENEWVI
                     AFTADHGQGPDPKAVGAWPIGQALIEEDIAEEFGVDPAELFQDRRPVGWWIHPEVSAE
                     TGVTAADLADFLVQYRLKDNKRPDPIPEEYRGMEDSLLFSAAFPSEELPRIWHCAKNR
                     A"
     gene            6804..7553
                     /locus_tag="VEV43_10360"
     CDS             6804..7553
                     /locus_tag="VEV43_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HYP23967.1"
                     /translation="METVEVLGVPVARLTAAAARQELVRLYERDRPALVAYANAHTLN
                     LASRDSGYREVLRSADIVLNDGAGLAIAGRVFGRPFPENLNGSDFNPAILEEAAARGW
                     PVYFLGAKPGVAEEASRRLSERIPGLRVAGTRDGYFAPSESDEVARSIAEAGAGVLMV
                     AMGNPLQEMWLKRHLEATGARLGVGVGAFLDFTAGVVPRAPAWMNKLGIEWVYRLLQE
                     PRRMWRRYVLGNPLFLARVLAQRVARGKTDE"
     gene            complement(7567..9261)
                     /locus_tag="VEV43_10365"
     CDS             complement(7567..9261)
                     /locus_tag="VEV43_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP23968.1"
                     /translation="MPSRPSGRTSPTLSFRKRRKSRALPVALVLLVGLAGGGGYLLLR
                     SSGDEGPAAKEKLVRACDVDVEILERMWNGYVPNRSGDVIAVEHAPNQYSTRHSSPWP
                     YHQDVPLVLYGPGFIRSGRTVDRPVTVADLAPTYAKLLGFDEFPERDGRVLQEALKPE
                     RKRNGVPKLILTLVWDGGGDNVLEQWPDDWPELKALLSRATEYKDATVGSTPSITPSV
                     HATIGTGVFPHTHGITDTRMRVKGKMADPWEESSPQRLRSQTLGDLWDAANGNVPLVG
                     MMARDAWHLGMVGHGAYLPEGDHDHAVMDELGGIEFRTNEEYYSLPEYLLGLEGLEEA
                     VAEIDGRDGEADGEWLGNPILAHDAQVRFTPAWSIFQTQKMVELLETEGYGTDDVADL
                     FYVNYKPVDLAGHFWNMVEPEVQENLRETDAQLPLIIEALDRLVGPDSYVIALTADHG
                     MTPYAQDSGGWDIETRDMSADIEKRFDRVTPDKPLILSNRGYQIMLDRKEMERNDVTA
                     AEVAEYVRDYRISDNVTDTNKVGERFEGRENERLFLTALTPEALEEALACARGRDD"
     gene            complement(9274..10812)
                     /locus_tag="VEV43_10370"
     CDS             complement(9274..10812)
                     /locus_tag="VEV43_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYP23969.1"
                     /translation="MEGRTFRQRVLFPAGVLLLLVAFVVTATVALESDPSDDAEVAAA
                     PKGRYRFRDVTRSAGVADAGRTWGSSWIDFEDDGDPDLFVVRHWREPMLYLNDGGEFD
                     PWTVPAELRGTHMDRHQCTWGEGNHDGRPDLLCVQGADNGQGEGPNQLLVNTGDGLRN
                     RAAQLRIQYAKGRGRTANWVDYDGDGDLDAFEGTTERPRFPMQLFKRGRENFKKARAG
                     LSHELSVLSSTWADWDRDGDFDLLVNQHYPFVTVAYENLAGKEFRPVHLPRITGKKWI
                     SASWGDFDADGWIDLQLAGRKRLRIYRNKKGRLRPVYKTRLKQGRAGTWFDVDNDGDL
                     DSFVVQGARGNHPKDDSVNWRDFLVIRRKGGFKRVEEGSFRGPTEGNGEQATIADYDR
                     DGRLDVFVTNGLHYYQGPTTLLRNEMNAGSWGGVDLAGSRKNPMGYGTVVKWRIGKRV
                     FKRLLTEEVVFKGQSEVGYVHLGFGDATAADVKLKWPDGDVDCTRLPAGTVLSIEKGS
                     LGCP"
     gene            10932..11903
                     /locus_tag="VEV43_10375"
     CDS             10932..11903
                     /locus_tag="VEV43_10375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYP23970.1"
                     /translation="MPPKTDQAEAARAESSFVIATRNRPDDLLKTVRSLVAQTVLPAE
                     LVVVDSSDETPTRAEIEALCSSAGIKLDYHHPAPRGLTVQRNVGNDRSTGDPVFFVDD
                     DVWLDEDCHEEVLKEYAKWGPELGGVRATPKRPARPSLGTRIYRRIFGIGGWWPEASG
                     KVRPGFYAEGVSDSAGVRKLEYFNGWFMSYRREVFEHERFDEALAGYGYKEDIDFSYR
                     VSRRYTLVQTPAARCDHLKSDASRMNSHQLQRMNLANQFYLHRKLMPQTLFHKAALWW
                     AWIGLFILNAGKAAQTRDPGLVTGMVAGAWEQIRGRGLVDPAAEKRA"
     gene            11903..>12240
                     /locus_tag="VEV43_10380"
     CDS             11903..>12240
                     /locus_tag="VEV43_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYP23971.1"
                     /translation="MAAAAPPGAKKRDYWWTVLATDPVAVPLVGFLARRRWLTPDQVT
                     AFALVSGLAVGVAFATGTRLGLVVGGLLFYVAFLFDCVDGKLARALGVTSAKGEALDH
                     LADGGRRASAS"
BASE COUNT         1745 a         4137 c         4249 g         2109 t
ORIGIN      
        1 agatctcctg cgcggtctgg ccgacctcgt cgtagtagta gcgcacccat cccgggatgc
       61 tctcgacggt gtggcacacg taagccacga ccgggtccgg cgagtcgcac acctcgggct
      121 gggcctgcgc cggcgacgcg atcatgaggg ccgacgcggc cagcaccccg gccaggccgg
      181 tggagacgct cttccgcaaa ccgtcctcct ctgtcagcag ttcggcgaga ccgtgcagta
      241 cgcgaagtga actgtgtcga acgccaggtt gtagtagtgg gtgaccgtag ggccgggctc
      301 cggcacgctg cccgccacgc ggcacgcgaa caggatgatc gggtcgtccg tcgagatgat
      361 ctcgtcgcac gggcccgcgc ttgcgggcga cgccgggccc agcgcgatca tgccgccggc
      421 gagcgccgtc gcgaggaaaa cccctagctt ccttctcatc ttggcctcct gatcgggcgg
      481 cgttctagca gtacggcgag acctggcagt acgcggtgtg gacggcgccg gtgacctggt
      541 agtagtagtg gttgatcgtc gggcccggct cgggggccga gttgatgatc cggcagacgt
      601 aggccagcac ggggtccgtc gccgcgcagt tggggtctgg ctgtgcatgc gccggtgagg
      661 cagtcatgga catcgttgct ccggccagca acccgaccgc gacgagcgac atcttcttcc
      721 gcatgtcttt ccccctgctt gtcgggggcc ccagtgcccc cacccgcccc agctattcgg
      781 ggaacccccc ggttttcctc ccgatttgac cggattcgac ccggttctct accgaacccc
      841 gggtatggac ctcccgatgg cgaggatcgc tcgttcggtg tgcgcttccg ttctcgtggc
      901 cacggccgtc ttcggtggcg aggcggccgc cgcgcgcccc ctggtgtgcc aggaccacga
      961 ggtcacgatc gccgggacgc cgggcgacga ccgtctcgcg gggaccaagg gagcggacgt
     1021 catcgcaggg ttgggcggca acgacgtcgt gcgcggcgtc ggcggcaacg acctgatctg
     1081 cggcaacgcg ggcgacgaca tgctgcgcgg cggccgcggt gacgacgggc tggacggggg
     1141 ccgcggggac gacgggctca ggggcgggaa gggcttcgac ctcgtgacgt tcgagcgtgc
     1201 tgcgggcgga gtgatcgtcg acctcaccgg gggccgggca tccggcgacg ggatcgacac
     1261 gatcgagtcg gtgtggtggg tcgtgggatc gccgcatgca gacgcggtca acggcgggcc
     1321 cgacatgaac gtcgtgatcg cgctcggcgg gcacgacgtc gttgcgggcc gcggcggcgg
     1381 ggacctcctc ctcggcggcc ccggccgcga ctcgctggcc ggaggcaagg gctgggatcg
     1441 tctgctcggc gagggcgggg acgacgcgct gcggggaggt ctcggcgagg acaccgtcag
     1501 ctacgcgagt gcgcgcgacc ccgtctccgt cgacctcgag aggggccggg ctcggggtga
     1561 aggacgcgac tcgctgtcca tggtcgagaa cgtagagggc tcgccgcagc gcgacgtgct
     1621 cgaaggcgac ggaggcccga acgagatcct cggtgcggag gccggcgacc ggatccgggg
     1681 ccgcggcggg aacgaccgca tcctgggcct ggggggagac gaccggctat ggctgggcgc
     1741 gggtgacgac ttgggccgcg ggggccgggc cgacgactgg atagacgggg gcctggggat
     1801 agaccgcgtg tacgggggcg cgggccacga cgtatgccgc aacggcgagg tcctgggctg
     1861 cgacgagtac gagctgccgg tgccgcccta ggagcccgtc aaggcgggag cgggtaaggg
     1921 cacagtgctg ctcgtgacga cgcgtcgcgg cgccgagcgg gcctggtgcc tgcgtctact
     1981 cgaagcctct gatctcctgc gcgagctcat cgatggtccg tgcgtcgaaa gattcctcga
     2041 acggaccggg tgtggtgttg agcggcgtcg tgaggccacc gtcgtgctcg atgaacagcg
     2101 ccttgtgcgt agtcaacgca tagagggtct tgccggggtc tcgctcggcg cccgattcgt
     2161 cgatctcgac gttcggaagc gttcccgctt ggtcgtagat cagaagcaac atccggtcgt
     2221 cgggagtcgc gtcgtccagc tcgttgacgc tgaccaatga cggacgccgt agctcgaggt
     2281 cgacgatcct tcggctgtcg gggttcagct gtcctcgtac gacctcgtcg acctggaagg
     2341 tcacgatcac cgactcggtc gtgacatctt gccccttctt cccgccgagc ccccgcccct
     2401 cgcggacgtt cttcatgtgc ccgatcacgg cgacgtccga gtccccccac agcgcttcga
     2461 gggtgtcgtg atactcgtag tcgaccgcga gccggttgat caaagaccag aagctcgggc
     2521 cggggctcga acgtccggaa gtgccctcgg gggcgccgca agcgccggag acgaacacga
     2581 cggccgagac gatgagcgcg agtctgcctc ctcgagtcat catgacagtc ccctcactcg
     2641 cgacctttct cagtagtggt tgttgatgtg agtcttgtcg tggtcggcga agaatcgagg
     2701 agggccgtcc aacccctgtt ccatgcagct ggatatctga taagcgtggt cgaggccgac
     2761 cgaatgcccc atctcgtgac atgcaacttt gtctttgttg tgctggcccg ccccgtcgag
     2821 gctgcctcgg ttgagggtca gcttcgccgt ttcgcattcc cctgcgctgt tcagcgcggt
     2881 gcagtcccat tcaccgtgga agcctgaacc acccacgtag tccgcgatca tccggacatc
     2941 cgtggctgaa gtcttcgtat cgactttctt cgtcgtcatc acggtcgcgt tgtcgaggtg
     3001 gccgacgatg tgatcgaaag cgtcgcgata cgactggttc ccgacaatct cgtaatacac
     3061 agtgtgttcg gcgttatcgg cgtactcgca acaaccatca acagggccga aatagttcgc
     3121 ttcagccctc ggaaggagag aggtgagggt cacgcccgcc aggaccgcga ccgtcagaag
     3181 tcttgtggcc ctcacgactt ctcctccccc ttggctcttg ttacatcact gtaacaagag
     3241 ttcctaccaa gggacaaact tcgctgcgag ccgaggcgcg cgttgggggc ccggtgccgc
     3301 cttagccctc ggctagggcg ttggtgcact ccactatctt cggcagcttg cggcccggga
     3361 agaccgcgtc gaagatccgt tcgtccaggc ggtcggcgaa gccgtcgggg atctcggccc
     3421 cctcggggat gttgtcgccg atcgtgtagc gcgacaggaa cgagctgacc tgctccggcg
     3481 tcaccccgtt ggcttccatc tcgtcggggt tgctgaagaa cgagctcgcc gaggtccggc
     3541 cgatcacgcc cttctcgttc gcgatcccgt cgaactggcg gccgaggtcg ccggagatct
     3601 cggagcggct gatcgcccag ccccccagcg gcgtctgccc gtggtcggcc gtgaccacca
     3661 gcacgtactc ctcttcgcca acgttgtcgt cgagccaccc gacgagctcg cgcagcgcgt
     3721 cgtcgaccga cttgatcacg tcctcttgct cgggggcgag catgttccac gtgtgccccg
     3781 ccttgtcggg ggccttgtag ttcaggtaga agaggtcggt catctcgtct ttgccgaagc
     3841 cctcgcgctc catgatctcg aacatcgtgt cgttctccca cggcgcgaac gcgggcgacc
     3901 cgtcgagcgg ctcgacgtcg tgcccccgcc acttcccgtc ggcctggccg tcggcgcggt
     3961 cgacctcctc ctggtacgtc tcggggccgg gcacgtcggt gttgacgtac tcgggcagcg
     4021 agtagaactc ggggttcgtc gcccactcgc cgcgcgacat gatcgcggcg acgtccttgt
     4081 ccccaccctg gagggccgag ccgtgcccga tcatcccgag ctgcaggttg ccggcggcga
     4141 gcatcccgat cagcggctcg ttgccgttct ctctgtccca gacgtcggcg agcgtgttct
     4201 tctccatcgt cacgcgcgcg tccatcaccg cggggccggg gttgccgggc acctcggaga
     4261 acgagcccac gatcgagccg tcgagcgcac gaaccgcgat cgcagtgacg ccgtggtagc
     4321 gggggaacgt ccccgtcgag aggttcgtgt gcgtcgccgg cgtgatcgac ggcgacgagc
     4381 cgacgatcgc ctcttcgacg ttcagaccct ccgagatcat ctccttcatg aacggccacg
     4441 cgtcgggcca ctcctcgagc gcgttccacc cgccgccgtc gatcaccgcg gtgacgatca
     4501 ggttcggcgc gtcgtcggtc tccttcaaga ggtcggccag cgacgtcgcg gtgcggtccg
     4561 gccactcgtc gaacttcatc aggtcggcgt aggtcggcgc gagatcggag atcgtgggct
     4621 cgcggtccac cgctacgtga ccggtcgggt cgacgaagcc cttgccgtag aagatcaggg
     4681 ggaccttctg caggaagtcg tacggcgcgg agtgcgacgt gttcacgaac gtgcccatgt
     4741 agttcggggg cttcggcacg atcacgaggt cgtagtcgcg gattccgccg ggggcccagc
     4801 cccggtggat gcgcttgacc cacttgggcg ggagcttgca ggcgtcgcgt agataggcct
     4861 tcgtcgggag gtcgacggcc ggctcgccgt cctcggtctt gagcgcctcg gtagtcggcg
     4921 ccggcggctc gcgatcgggc tcgccgtccg agcgggtcag cacgtaccca ccgccgccga
     4981 gcaagaccgc gacgacgagc agcgggagcg cgcggctgcg cctcctcctg cgcgcctccc
     5041 tcatgcccgg ttcttggcgc agtgccagat gcgtggcagc tcctcggacg ggaacgccgc
     5101 ggagaacagc agcgagtcct ccatcccgcg gtactcctcg gggatcgggt cgggccgctt
     5161 gttgtccttc aggcggtatt ggacgaggaa gtccgcgagg tccgcggccg tgacgccggt
     5221 ctcggccgac acctccgggt ggatccacca gccgacgggc cggcggtcct ggaagagctc
     5281 ggccgggtcg actccgaact cctcggcgat gtcctcctcg atcagggcct ggccgatcgg
     5341 ccacgcgccg acggccttcg ggtccggccc ctgtccgtgg tcggcggtga acgcgatcac
     5401 ccactcgttc tcgccgacct gctcgtcgag gaactgcacg atcttgcgga gctccttgtc
     5461 cgagtagacg aggatctcgc gcgtctccgg gttcagcatg ttccagtcgt ggccgacgtc
     5521 gtcgatctgc ttgtagttga tgaagaagag gtccgcgacg tcgtcgccgc cgaagtcctc
     5581 accctcgagc actgccttca acagacgcgt ctggtacagc gcccacaccg gagtgtggcg
     5641 gagcttgttc gggtcgttga ggacctcgtg gcccatccag aggtcgtcga tcttgccgtc
     5701 gtcgaggtcg accttgcgca cgtcctcttc gaacccttcg acgtcttcta ggtaaggcgg
     5761 gagtgagtag tactccgggt tcgtcttgag ctcgccggcg aacttctcgg agatgaacgc
     5821 gatgtccttg tcgccgccgg ggacgtagct gccgtgtccg atcatgccga ggtgccacgg
     5881 cttgtaggcg aacatgccga tcttcgcctc gttgccggtc ctgcggtcgt agaggtcggc
     5941 gagcgtcggc acctgcagga accgcggcgt gaagccgtcg tacgcgccga acagctcgac
     6001 gccctcgcgg acggggatgt cgacgatccc gtgctgcttc gggaacgtgc cggtgccgag
     6061 gttcgtgtgg acggccggcg tgaccgaagg cgacgacccg acctcggtgt tcccgatcga
     6121 cgtccccttc tccatcatct tcttcaggta cggccacgag tccggccacg cctcgaggac
     6181 gttccatccg ccgccgtccc acacgatcgt cacgatgagc ttcgggatcc cgttgcgctc
     6241 ctcttcgggg agcagcgcct cggtcaccgg gttcccgggc cggcccccgg ggaacggcgt
     6301 gtcgaggagc tccgcgagcg tcggcgcgag gtcggcgatc gtcgtctcgc ggtcgagcgt
     6361 gatctcgccc tgctccttga tgaagccggg gccgtagaag acgagcggga tcttctgcac
     6421 gtagtcccac gggcccgagt gcgtgtacga cgcgaagctg ccgaagtagt tgggctcgcg
     6481 ggggacgacg aggagctcgg gcgagcggcc ggggacgtag ccgcggcgca cgcgcttcag
     6541 ctgctcgagc gggaggttgc acgcggcctc gaaccatccg tccggggcct gggtgacggg
     6601 ctcgggggag gcgatggggg cccggccctg gttgggggcc tggctgggcc gcggggtcgc
     6661 ctcggctcgg ggctcttcgg cgtcgctgtt cgtacacgct gccgcgagca acaacgcgac
     6721 aaccgccgca accgcttttc tcatcgccct gtccactccc gaggtagtcg atccgttccg
     6781 cgagccagtt acggtgcacc accgtggaga cggtcgaggt gctcggcgta ccagtggctc
     6841 gcctcacggc ggccgctgcg aggcaggagc tggtccgtct ctacgagcgg gaccgcccgg
     6901 ccctcgtcgc gtacgccaac gcccacacgc tcaacctggc cagcagggac tcagggtaca
     6961 gggaggtcct tcgttcggcg gacatcgtgc tgaacgacgg ggcggggctc gcgatagccg
     7021 gccgggtctt cggccgcccg ttcccggaga acctcaacgg ctccgacttc aacccggcga
     7081 tcctggaaga agctgccgcc cgcgggtggc cggtgtactt cctgggggcg aagcccggtg
     7141 tggccgagga ggcgtcccgg cgtctgtcgg agcggatccc cggcctgcgc gtcgcgggga
     7201 ctcgtgacgg ctatttcgcg ccgtcggaat cggacgaggt cgcgaggtcg atagccgagg
     7261 cgggggccgg ggtgctgatg gtggcgatgg gcaacccgct acaggaaatg tggctgaagc
     7321 gccacctgga ggcgacgggg gcgagactcg gcgtcggcgt aggtgcgttc ctggacttca
     7381 cggcgggggt ggtgccgagg gcccccgcat ggatgaacaa gctggggatc gaatgggtgt
     7441 accggctgct gcaggagccg cgccgcatgt ggcgccgcta cgtcctgggc aacccgctgt
     7501 tcctcgcccg cgtgctcgcg caacgcgtcg cgcgaggaaa aacggacgaa tagcgtccga
     7561 tttccctcag tcgtcgcggc cccgggcgca ggccaacgct tcctctagcg cttccggcgt
     7621 cagggccgtc aggaacagac gctcgttctc gcggccctcg aagcgctcgc ctaccttgtt
     7681 cgtgtccgtc acgttgtccg agatccggta gtcgcgcacg tactcggcta cctcggccgc
     7741 ggtcacgtcg ttgcgctcca tctccttgcg gtccagcatg atctggtagc cgcggttcga
     7801 caggatcagc ggcttgtccg gggtcacccg gtcgaacctc ttctcgatgt ccgcgctcat
     7861 gtcgcgcgtc tcgatgtccc agccccccga gtcctgcgcg tacggcgtca tcccgtggtc
     7921 ggccgtcagc gcgatcacgt acgagtccgg gcccacgaga cggtccaggg cctcgatgat
     7981 cagcgggagc tgggcgtccg tctcgcgcag gttctcctgg acctccggct cgaccatgtt
     8041 ccagaagtgc cccgccaggt cgaccggctt gtagttgacg tagaagaggt cggcgacgtc
     8101 gtcggtgccg tagccctcgg tctcgagcag ctcgaccatc ttctgcgtct ggaagatcga
     8161 ccacgccggc gtgaaccgga cctgcgcgtc gtgggcgagg atcgggttgc ccagccactc
     8221 accgtcggcc tcgccgtcgc gaccgtcgat ctcggcgacg gcctcttcga gcccttccag
     8281 ccccagcagg tactcgggca gcgagtagta ctcctcgttc gtgcggaact cgatcccgcc
     8341 gagctcgtcc atcaccgcgt ggtcgtggtc gccctcgggc aggtacgcgc cgtggccgac
     8401 catcccgaga tgccacgcgt cgcgcgccat catcccgacc agcgggacgt tcccgttcgc
     8461 cgcgtcccac aggtcgccca gggtctggga gcggaggcgc tgcggcgagc tctcctccca
     8521 ggggtccgcc atcttgccct tgacgcgcat cctcgtgtcg gtgatcccgt gcgtgtgcgg
     8581 gaacacgccg gtgccgatcg tcgcgtgcac cgacggcgtg atcgaaggag tcgagccgac
     8641 cgtcgcgtcc ttgtactcgg tcgctcgcga cagcagcgcc ttgagctccg gccagtcgtc
     8701 cggccattgc tcgagcacgt tgtcaccacc cccgtcccac acgagcgtga ggatcagctt
     8761 cggcacgccg ttgcgcttgc gctcgggctt caaggcctcc tgcagcacgc ggccgtcgcg
     8821 ctcggggaac tcgtcgaagc cgaggagctt ggcgtacgtg ggcgcgaggt ccgcgacggt
     8881 gaccggccgg tcgacggtcc ggcccgaacg gatgaagccc ggcccgtaca gcacgagcgg
     8941 gacgtcctgg tggtacggcc acggcgacga gtgccgggtg ctgtactggt tgggcgcgtg
     9001 ctcgaccgcg atgacgtcgc cggagcggtt ggggacgtag ccgttccaca tgcgctcgag
     9061 gatctctacg tcgacgtcgc aggcccggac gagcttctcc ttggccgcgg gcccctcgtc
     9121 tcccgacgag cggagcagca ggtacccgcc gccgccggcg aggccgacga ggaggacgag
     9181 cgcgaccggc agggcgcggc tcttccggcg cttgcggaac gacagcgtgg gagacgttct
     9241 tccagagggt cgagagggca tggcgggcct accctatggg cagccgaggc tccctttctc
     9301 gatcgagagc accgtcccgg cgggcagcct cgtgcagtcg acgtccccgt ccggccactt
     9361 cagcttgacg tccgcagccg tcgcgtcgcc gaagccgagg tggacgtagc cgacctcgct
     9421 ctggcccttg aacacgacct cctcggtcag gaggcgcttg aagacccgtt tgccgatcct
     9481 ccacttgacg accgtcccgt agcccatcgg gttcttgcgc gagccggcga ggtcgacgcc
     9541 gccccagctc cccgcgttca tctcgttgcg cagcagggtc gtgggcccct ggtaatagtg
     9601 caggccgttc gtgacgaaga cgtcgaggcg tccgtcgcgg tcgtagtcgg cgatcgtcgc
     9661 ctgctcgccg ttgccctcgg tcgggccgcg gaacgatcct tcctcgacgc gcttgaagcc
     9721 ccccttgcgc cggatgacga ggaagtcgcg ccagttcacc gagtcgtcct tggggtggtt
     9781 gccgcgcgcg ccctggacga cgaacgagtc gaggtccccg tcgttgtcga cgtcgaacca
     9841 ggtcccggcg cgcccctgct tcaggcgcgt cttgtagacg ggacgaagcc ggcccttctt
     9901 gttgcggtag atgcggagac gcttgcgccc ggcgagctgc aggtcgatcc acccgtccgc
     9961 gtcgaagtcc ccccagctcg cggagatcca cttcttgccc gtgatgcgcg gcaggtggac
    10021 cggccggaac tccttgccgg ccaggttctc gtaggcgacg gtgacgaacg ggtagtgctg
    10081 gttgacgagc aggtcgaagt cgccgtcgcg gtcccagtcc gcccacgtcg aagacagcac
    10141 cgagagctcg tgcgagagcc cggcgcgcgc cttcttgaag ttctcccggc cccttttgaa
    10201 cagctgcatc gggaaccgtg gccgctcggt cgtcccctcg aacgcgtcga ggtcgccgtc
    10261 gccgtcgtag tcgacccagt tcgcggtccg gccacggccc ttcgcgtact ggatccggag
    10321 ctgcgcggcg cggttccgca acccgtcacc ggtgttgacc aggagctggt tcgggccctc
    10381 gccctgcccg ttgtcggccc cctggacgca gaggaggtcc ggacgcccgt cgtggttgcc
    10441 ttcgccccag gtgcactggt gacggtccat gtgggtccct cgcagctcgg ccggcacggt
    10501 ccacgggtcg aactcgccgc cgtcgttgag gtagagcatc ggctcgcgcc agtggcgcac
    10561 gacgaagagg tcgggatcgc cgtcgtcctc gaagtcgatc cacgagctgc cccacgtacg
    10621 tcccgcgtcg gcgacgccgg cggagcgcgt gacgtcgcgg aagcggtacc tgcccttcgg
    10681 agcggcggcg acctcggcgt cgtccgatgg gtcgctctcg agcgcgaccg tcgccgtcac
    10741 gacgaacgcg acgagcagca ggaggacgcc cgcggggaac aggacgcgct ggcggaacgt
    10801 gcgtccttcc aagtcgccac ctgcccgggc tcggtcgtag tttccgtcgg gtcgtgccac
    10861 ctccggtgac acgaacgagc cgggaggatc gttcccacag tcccggcttc tctgacggta
    10921 ccctgcctcc cgtgccccca aagacagacc aagccgaggc ggctcgggcc gagtcgtcgt
    10981 tcgtcatcgc cacccggaac cggcccgacg acctgctgaa gacggtccgg agcctcgtcg
    11041 cgcagacggt gctgccggcc gagctcgtcg tcgtcgactc gagcgacgag acccccaccc
    11101 gggccgagat cgaggcgctg tgctcgtcgg ccgggatcaa gctcgactac caccatccgg
    11161 ccccccgcgg cctgaccgtc cagcgcaacg tcggcaacga ccgctcgacc ggcgaccccg
    11221 tgttcttcgt cgacgacgac gtctggctcg acgaggactg tcacgaagag gtgctgaagg
    11281 agtacgccaa gtggggaccc gagttgggtg gcgtgcgtgc gaccccgaag cggcccgccc
    11341 ggccctctct ggggacccgc atctaccggc ggatcttcgg gatcggcggc tggtggcccg
    11401 aggcgagtgg gaaggtgcgg cccggcttct acgcggaggg cgtctccgac tctgcgggcg
    11461 tccgcaagct cgagtacttc aacgggtggt tcatgtcgta ccgccgggag gtcttcgagc
    11521 acgaacgctt cgacgaggcc ctcgccggct acggctacaa ggaggacatc gacttctcct
    11581 accgggtgtc gcgccgctac acgctcgtcc agaccccggc ggcgcgctgc gaccacctca
    11641 agagcgatgc gtcccgcatg aactcgcacc agctccagcg catgaacctc gccaaccagt
    11701 tctatctgca ccgcaagctg atgccgcaga cgctgttcca caaggccgcg ctgtggtggg
    11761 catggatcgg gctgttcatc ctgaacgccg gcaaggccgc gcagacccgc gaccccggcc
    11821 tcgtcacggg gatggtcgcc ggcgcgtggg agcagatacg gggccgcggg ctcgtcgacc
    11881 cggctgcgga gaagcgcgcg tgatggcggc ggctgcgcct ccgggcgcga agaagcgcga
    11941 ctactggtgg acggtcctcg cgaccgaccc cgtcgcggtc ccgctcgtcg ggttcctcgc
    12001 gcggaggcgc tggctgacgc cggaccaggt gaccgcgttc gcgctcgtgt cggggctcgc
    12061 agtcggggtc gcgttcgcga ccggcacgcg cctcggcctc gtcgtcggag gtctgctgtt
    12121 ctacgtcgcg ttcctgttcg actgcgtaga cgggaagctc gcacgcgcgc tcggcgtcac
    12181 gagcgcgaag ggcgaggcgc tggaccatct cgcggacggg gggcggcgtg cgagcgcgtc
//
LOCUS       DASQDO010000369         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_957074, whole genome shotgun sequence.
ACCESSION   DASQDO010000369 DASQDO010000000
VERSION     DASQDO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957074"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..939)
                     /locus_tag="VEV43_10385"
     CDS             complement(262..939)
                     /locus_tag="VEV43_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYP23972.1"
                     /translation="MDLESVDHLLTTTRTVRKRLDLERAVERDVLLECLRIAVQAPTG
                     SNLQSWRWVVVDDEATRRQVGEIYSRAIGPYNSFWDQSIDPANKSLRRVVDSSIYLEQ
                     ILGRVPVLVIPCALGTPAQSETFFEGLGYGGDSLRNLVASGFYGSIWPAVWSFMLALR
                     SRGLGSALTTMHLALEREMGELLGIPDTVTQVGLIPVAYYTGDDFKQAKRRPPEEITY
                     FNGWKQR"
     gene            complement(939..1712)
                     /locus_tag="VEV43_10390"
     CDS             complement(939..1712)
                     /locus_tag="VEV43_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23973.1"
                     /translation="MAIVAGACADSPGRADLGTPEERATQAEAQREPEPGRLTARPSP
                     DVPKRARTGLFELSVGEGRQPLLFVPESYDPRGPAPFALTLHGANAGAPNGMKRLLPY
                     AEKEGLILLAVASRERTWDVIYGGFGPDVEHIDAALTEVFSKYAIDAQHLAAQGFSDG
                     ASYALSLGITNGDLFSHVIAFSAGFMAPGKAHGSPRIYTSHGTTDPILPIGSTSRKYV
                     PQLERAGYDVTYTEFEGGHRTPQYVATEAVEWFLREGGR"
     gene            complement(1745..1942)
                     /locus_tag="VEV43_10395"
     CDS             complement(1745..1942)
                     /locus_tag="VEV43_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23974.1"
                     /translation="MNTAELSPAQRELVDCYQRLVTILAEHRDELAPFEERNASKATA
                     ALWQVVNGLDLDPGHLYHVGV"
     gene            complement(1939..>2508)
                     /locus_tag="VEV43_10400"
     CDS             complement(1939..>2508)
                     /locus_tag="VEV43_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HYP23975.1"
                     /translation="GGTHIIEPHYIEEVIDPESGDPVPYGEQGERVVTSFGRGFIPVI
                     RYRTRDLVVKVPADRCTCGRTLDLYEGGIVGRVDDMKLVRGTNIYPRAIESIVREHEA
                     VDEFQIHLYTAEGIRDEIEVLIEVPDESVDAEGLVRELGTALSEAHEGLRFGVRTVEL
                     GSLPRFELKAKRLVDERVLAGSEGERKSM"
BASE COUNT          376 a          875 c          838 g          419 t
ORIGIN      
        1 acggggtgta gatcgtgcac ccggtgccgg ggatgggtac cgcggccgac ccgatctcgg
       61 ggctgtcggc cgcctgtgcc ggagccgcgc ccatgacgcc cgccgccatc gccgctgcaa
      121 gaaccagact tgacttacgc attgtccgcc tcccggttac tcgtactgct ttcgccaccg
      181 gggatatacc aaaacggcag aaatcgtgtc aagaagtcgg tcagaaaagt tgagtgcatc
      241 ttcacccgat taggtgacgc gtcagcgctg cttccagccg ttgaagtacg tgatctcttc
      301 gggcgggcgg cgtttggcct gcttgaagtc gtcccccgtg tagtacgcga ccgggatcag
      361 gccgacctgc gtcaccgtgt ccgggatccc cagcagctcg cccatctcgc gctccagcgc
      421 caggtgcatc gtcgtcagcg cgctgcccag ccccctgctc ctcagcgcga gcatgaagct
      481 ccacaccgcc ggccagatcg agccgtagaa gcccgacgcc acgaggttgc gcagcgagtc
      541 gccgccgtag ccgaggccct cgaagaacgt ctcgctctgc gcgggcgtgc cgagcgcgca
      601 cgggatcacg agtaccggga cgcggccgag gatctgctcg aggtagatcg acgagtcgac
      661 gacgcggcgc aggctcttgt tcgccgggtc gatcgactgg tcccagaacg agttgtaggg
      721 gccgatcgcc cggctgtaga tctcgccgac ctgacgccgt gtggcctcgt cgtccaccac
      781 gacccagcgc cagctctgga ggttcgaccc ggtcggggcc tggacggcga tgcgcaggca
      841 ctcgagcagc acgtcccgct cgaccgctcg ctcgaggtcg agtcgcttgc gcaccgtcct
      901 cgtagtcgtc agcaggtggt cgacgctctc gaggtccatc aacggcctcc ttctcgcagg
      961 aaccattcga ccgcttcggt cgcgacgtac tggggcgtgc ggtggccgcc ctcgaactcg
     1021 gtataggtga cgtcgtagcc ggcgcgctcc agctgcggga cgtatttgcg gctcgtcgac
     1081 ccgatcggca ggatcgggtc ggtcgttccg tgcgacgtgt agatgcgcgg cgagccgtgg
     1141 gccttgcccg gcgccatgaa cccggccgag aacgcgatca cgtggctgaa gaggtccccg
     1201 ttcgtgatcc cgagcgacag cgcgtacgac gcgccgtcgg agaagccctg cgcggcgagg
     1261 tgctgcgcgt cgatcgcgta cttcgagaag acctcggtca gagcggcgtc gatgtgctcg
     1321 acgtcggggc cgaagccccc gtagatcacg tcccaagttc gttcccgcga cgcgacggcc
     1381 agcagtatca agccctcttt ctccgcgtac gggagcaggc gtttcatccc gttcggagcg
     1441 cccgcgttcg cgccgtggag cgtgagcgcg aagggggccg ggccacgcgg gtcgtacgac
     1501 tccggcacga acagcagcgg ctgccggccc tcgccgaccg atagctcgaa caagccggtg
     1561 cgtgcgcgtt tcgggacgtc agggctcggg cgcgcggtca gccggcccgg ctcgggctcc
     1621 ctctgggcct cggcctgcgt cgcccgctcc tcaggcgttc cgaggtcggc gcggcccggc
     1681 gagtccgcgc aggcacccgc gacgatcgcc acggccgaca gtgcgatcgc gaacgctcgg
     1741 acggtcagac acctacgtga tagaggtggc cggggtcgag gtcgagcccg ttgacgacct
     1801 gccacagcgc ggcggtcgcc ttggacgcgt tgcgctcctc gaacggtgcc agctcgtcgc
     1861 ggtgctcggc caggatcgtc accaggcgct ggtagcaatc gacgagctcg cgctgcgccg
     1921 gagacagctc tgcggtgttc acatcgactt cctttctcct tcgctgccgg ccaggacccg
     1981 ctcgtctacc aagcgcttcg ccttcagctc gaagcgcggc agcgagccga gctcgaccgt
     2041 gcgcacgccg aacctcaggc cctcgtgggc ctcggacagt gcggtcccca gctcgcgcac
     2101 gagcccctcg gcgtccacgc tctcgtcggg cacctctatc agcacctcga tctcgtcgcg
     2161 gatgccctcg gcggtgtaga ggtggatctg gaactcgtcg accgcctcgt gctcgcggac
     2221 gatcgactcg atcgcacgcg gatagatgtt cgtcccgcgc acgagcttca tgtcgtcgac
     2281 gcggccgacg atcccgccct cgtagagatc gagggtccgc ccgcacgtgc agcgatcggc
     2341 cgggaccttc acgacgaggt cgcgcgtccg gtaccggatg accgggatga acccgcggcc
     2401 gaacgacgtg acgacgcgct ctccctgctc gccgtaaggg accgggtcac cgctctcggg
     2461 gtcgatgacc tcttcgatgt aatgcggctc gatgatgtgc gtcccgcc
//
LOCUS       DASQDO010000370         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1140861, whole genome shotgun sequence.
ACCESSION   DASQDO010000370 DASQDO010000000
VERSION     DASQDO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140861"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1241
                     /locus_tag="VEV43_10405"
     CDS             273..1241
                     /locus_tag="VEV43_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23976.1"
                     /translation="MTEATTAPAELEQELEAHRRELTAYAYRMLGSTFEAEDAVQDAL
                     LRAWRSYEGFEGRSAFRSWLYRIVTNVCLDMLNSRGRRALPMDLGPAQSADTPLPAPL
                     PEVTWLEPIPESHVSSGDPAEVAEMRESIRLAFVAALQHLPPRQRAVLILREVLRWKA
                     AEVAELLDTSVASVNSALQRARASMESVGATEPHQPEDEDQKALLARYVDAFERYDME
                     AFTALLREDATWSMPPFDLWLQTHDDIVKWCLGPGIACRGSKLIPTVANGMPAFGQYK
                     PSPDGGLAPWSLQVLSLSGGEVTGICFFLDTERLFPLFGLPGHLEA"
     gene            complement(1134..1553)
                     /locus_tag="VEV43_10410"
     CDS             complement(1134..1553)
                     /locus_tag="VEV43_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010984794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYP23977.1"
                     /translation="MSFGPHAGPIWWESRKETAVVLVINASITPRDLPELCELVRALL
                     AGAGSTVVCDVRALDRPDLCTVDALARLQLAVKRLGGSIRLCGATPQLQRLLELTGLG
                     AALGLEVAGQAEQREQALGVEEEADARDLAPREREDL"
     gene            complement(1645..1727)
                     /locus_tag="VEV43_10415"
     tRNA            complement(1645..1727)
                     /locus_tag="VEV43_10415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1692..1694),aa:Ser,seq:tga)
     gene            complement(1790..2263)
                     /locus_tag="VEV43_10420"
     CDS             complement(1790..2263)
                     /locus_tag="VEV43_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23978.1"
                     /translation="MKVFRGALLGVVAAVAVSSVALAANVHFKDDPVFTDQGTTLRAT
                     GSLAGLGNKDVTITLTATGTASDITCTNKGGKVAPGQNKPKVTTVGQTSIPVTEVKNG
                     NVSFAVETAQPFVTAKQAGCPNNNWTATVNDVDFTSATITVVQGGKVVLKETFAL"
BASE COUNT          351 a          788 c          786 g          381 t
ORIGIN      
        1 gtcggtgatc cacaacagct gcgagctgct cggcgcgagg tcccgcgtca gcgacggcac
       61 cgcgaggtac agcaccgaga ggtccatgac gtagagcagg caggcgagcg agatgacgcc
      121 cagcccgatc cattccttgc gtccggcttt gcccatctct ctcacctccg gttggggacg
      181 atctacgggc tacgacccgg ctgcgctcgg aaactcatcg cgcgggccgc gattggttcc
      241 gctcacttcc ggggtcgata gagttgatgg cgatgaccga ggccaccacc gcgcccgccg
      301 agctggagca ggagctcgaa gcacaccggc gcgagctcac cgcctacgcg taccggatgc
      361 tcggctcgac cttcgaggcc gaggacgccg tgcaggacgc gttgctccgc gcgtggcgga
      421 gctacgaagg gttcgaggga cgctctgcgt tccgttcgtg gctctatcgc atcgtcacga
      481 acgtatgcct ggacatgctg aacagcaggg ggcggcgcgc gctcccgatg gacctgggcc
      541 ccgcgcagag cgcggacact ccgctgcccg cgccgttgcc cgaggtgacg tggctcgagc
      601 cgatcccgga gtcgcacgtg tcgagcggcg atcctgccga ggttgccgag atgcgcgagt
      661 cgatccgcct cgcgttcgtc gcggcgctcc agcacctccc gccgcgtcag cgcgcggtgc
      721 tgatcctgcg cgaggtcctg cgatggaagg cggccgaggt cgccgagctc ctcgacacga
      781 gcgtcgcgtc ggtgaacagc gcgctgcaga gggcgcgtgc gtccatggag agcgtcggcg
      841 caaccgagcc gcaccagccc gaggacgaag accagaaggc tttgctcgcg cgctacgtcg
      901 acgcgttcga gcgctacgac atggaggcgt tcaccgcgct gctgcgcgag gacgcgacct
      961 ggtcgatgcc gccgttcgac ctatggctcc agacgcacga cgacatcgtg aagtggtgcc
     1021 tcggcccggg gatcgcttgc cgcggctcga agctgatccc gacggtcgcg aacgggatgc
     1081 cggcgttcgg tcagtacaag ccgtcgccgg acgggggcct ggcgccgtgg tcgctacagg
     1141 tcctctcgct ctcggggggc gaggtcacgg gcatctgctt cttcctcgac accgagcgcc
     1201 tgttcccgct gttcggcctg cccggccacc tcgaggccta gggccgctcc cagaccggtc
     1261 agctcgagga gcctctggag ctggggagtc gccccgcaca gcctgatcga gccgccgagc
     1321 cgcttcacgg cgagctggag acgggcgagc gcgtcgacgg tgcagaggtc cggccggtcg
     1381 agggcgcgga cgtcgcagac gacggtcgag ccggcgccgg cgaggagcgc ccgcacgagc
     1441 tcgcacagct cgggcaggtc ccgcggcgtg atcgaggcgt tgatgacgag tacgacggcc
     1501 gtctcctttc gcgactccca ccagatggga cccgcgtggg ggccgaaact catcgcgagt
     1561 gcacgagcgg cgcgtgcggc agagccttta ctaagggtcg gccttggtaa aggttagggc
     1621 gcagccttta ccaaggctag gcagcggagg gcgggggact cgaaccccca agtcctttcg
     1681 gacggcggtt ttcaagaccg ctcggttacc aattaccgta gccctccccg gcctagccta
     1741 cgcgcaaggg ccccggccgt agccggggcc ccgcagatct cccagcgcgc tagagcgcga
     1801 acgtctcctt cagaactacc ttgcccccct ggaccaccgt gatcgtcgca ctcgtgaagt
     1861 cgacgtcgtt cacggtcgcg gtccagttgt tgttcgggca gccggcctgc ttcgccgtca
     1921 cgaacggctg cgcggtctcg acggcgaacg acacgttccc gttcttgacc tcggtcaccg
     1981 ggatcgacgt ctggccgacc gtcgtgacct tcggcttgtt ctggcccggc gccaccttgc
     2041 cgcccttgtt cgtgcacgtg atgtcgctgg ccgtgcccgt cgccgtcagg gtgatcgtca
     2101 cgtccttgtt cccgagcccg gcgagcgagc cggtcgcccg cagcgtcgtg ccctggtcgg
     2161 tgaagaccgg atcgtccttg aagtggacgt tcgccgcgag ggcgacggac gagacggcta
     2221 ctgcggctac cactccgagc aatgctccgc ggaatacctt caagacttcc tcctcgtgat
     2281 ccgatgaata ttggcagtct ttacca
//
LOCUS       DASQDO010000371         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1201951, whole genome shotgun sequence.
ACCESSION   DASQDO010000371 DASQDO010000000
VERSION     DASQDO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201951"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /gene="gspM"
                     /locus_tag="VEV43_10425"
     CDS             complement(<1..288)
                     /gene="gspM"
                     /locus_tag="VEV43_10425"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HYP23979.1"
                     /translation="MNARTRLILTVVVSVIVLLLAYFLLIRPRQGELSDVQAQVETEE
                     NLTLQLRGQLEALEQLQRNAPQLQADLDRIRDLVPQDHEVPNFIFQVNAAAA"
     gene            complement(285..941)
                     /locus_tag="VEV43_10430"
     CDS             complement(285..941)
                     /locus_tag="VEV43_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23980.1"
                     /translation="MMRRIDLLPSAYQERRRERRNLGVVVVAAMLVLLLLIGWWFLLG
                     ADIRSEQDNLADAQSTNQRLQSEIAELSRFADLQAEVDAKRQALVTVMAGDVDWPAVM
                     TEIAMVIPGEVWLTAMNGSAGATEGATPVGTETAPVRVSGEAPFGRISFQGKSLTMPG
                     VAKWLVSLRNVREFSAIWLNTATASELEGTLTVDFDSTLELSDRAASRRYLDDVGEDQ
                     "
     gene            complement(938..1999)
                     /gene="pilM"
                     /locus_tag="VEV43_10435"
     CDS             complement(938..1999)
                     /gene="pilM"
                     /locus_tag="VEV43_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HYP23981.1"
                     /translation="MPFLSPPPIGLDIGTSAVRAAQVATGRGTPSLISFGQVALPQGA
                     VVDGEIRDAGAVSEAISQLWKRTKLRSKRAVLGVANQREVVSQVDLPYVAEKDFRASL
                     RFQVADHIPMPVESAELDFQVLEDFTTESGDHMMRVLLVAAATDMVESFVDAASAAGI
                     EPAGVDLAPFAVARAVSAAARRESGVAGAEAVIDVGAGVTNIVVHHNGEPRFVRILLV
                     GGDDASNALSEELGVSFDEAEATKLDLGRGVGSPEAQRVLNRQVAVLVEEIRGSLDYY
                     LSQEDSEPLSSVILTGGGSLTPGLSSHLEQTIRTQIQRGAVLSQVNAARSGLTEEQLA
                     QVEPVAAVAIGLALGATTR"
     gene            complement(1999..>2172)
                     /locus_tag="VEV43_10440"
     CDS             complement(1999..>2172)
                     /locus_tag="VEV43_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP23982.1"
                     /translation="LTVGEVAGIMRVSNMTVYRLIKSGQLSAIRVGKNYRIRRMDVDK
                     YLSDRAVRIEEDG"
BASE COUNT          330 a          752 c          763 g          327 t
ORIGIN      
        1 ggccgcggct gcgttgacct ggaagatgaa gttcggcacc tcgtggtcct gcggcacgag
       61 gtcgcgtatc cggtcgaggt cggcctggag ctgcggagcg ttgcgctgga gctgctcgag
      121 cgcctcgagc tgcccgcgca gctggagcgt caggttctcc tcggtctcga cctgcgcctg
      181 gacgtcggac agctcgccct ggcgcggcct gatcagcagg aagtacgcga ggaggagcac
      241 gatcacggag accacgaccg tcaggatgag ccgggtacgc gcgttcactg gtcctcaccc
      301 acgtcgtcga ggtaacgccg gctcgcggcc cgatcgctca gctcgagcgt cgagtcgaag
      361 tcgacggtca gcgttccctc gagctcgctc gccgtcgccg tgttcagcca gatcgcagag
      421 aactcgcgca cgttgcggag gctcacgagc cacttggcga cgcccggcat cgtcagcgac
      481 ttgccctgga acgagatccg cccgaacggt gcctcgccgg acacccggac cggagcggtc
      541 tcggtgccga ccggggtcgc gccctcggtc gcgccggccg agccgttcat cgcggtcagc
      601 cagacctcgc cggggatgac catcgcgatc tcggtcatca cggcgggcca gtcgacgtcg
      661 ccggccatga ccgtgacgag ggcctggcgc ttcgcgtcga cctctgcctg gaggtcggcg
      721 aagcgcgaca gctcggcgat ctcgctctgg agcctctggt tcgtgctctg cgcgtccgcg
      781 aggttgtcct gttcggacct gatgtccgcg ccgagcagga accaccaccc gatgagcagc
      841 agcagcacga gcatcgcagc gacgacgacg acgccgaggt tgcgccgttc gcgccgccgc
      901 tcctgataag cggagggaag aaggtcgatc cggcgcatca tcgggtcgtc gcccccagcg
      961 cgagcccgat cgcgacggcg gccacgggct cgacctgcgc aagctgctcc tcggtgagcc
     1021 ccgaccgcgc cgcgttcacc tgcgacagca cggcgccccg ctggatctgg gtccggatcg
     1081 tctgctcgag gtgactcgag agacccggcg tcagcgagcc gccgccggtg aggatgacgg
     1141 acgacagggg ctcggagtcc tcctgcgaca ggtagtagtc gagcgacccg cggatctcct
     1201 cgacgagcac cgcgacctga cggttcagca cgcgctgcgc ctccggggag ccgacgcccc
     1261 gcccgaggtc gagcttggtc gcctcggctt cgtcgaacga gaccccgagc tcttcggaca
     1321 gcgcgttcga cgcgtcgtcg ccgcccacga gcaggatgcg gacgaagcgc ggctcgccgt
     1381 tgtgatggac gacgatgttc gtgacgcccg cgcccacgtc gatgacggcc tcggcccccg
     1441 cgacgcccga ttcacgacga gcggccgcgc tcacggcgcg ggccacggcg aagggtgcca
     1501 ggtcgacgcc cgccggctcg atgccggcgg ccgaggccgc gtcgacgaaa gactcgacca
     1561 tgtccgtggc ggccgcgacg agcaggacgc gcatcatgtg gtcgccgctc tcggtcgtga
     1621 agtcctcgag gacctggaag tccagctcgg ccgactcgac cggcatgggg atgtggtcgg
     1681 cgacctggaa gcgcagggac gcgcggaagt ctttctccgc cacgtacggg aggtctacct
     1741 gactgacgac ctcgcgctgg ttggcgacgc cgagcacggc gcgcttggac ctcagcttcg
     1801 tgcgcttcca gagctgcgag atcgcctcgg agacggcacc ggcgtcccgg atctcgccgt
     1861 cgacgacggc gccctgtggc agggcgacct ggccgaacga gatcagcgac ggcgtgccgc
     1921 ggcccgtggc gacctgggcc gcgcggacgg cgctcgtccc gatgtccagg ccgatggggg
     1981 gcggggacag gaagggcatc agccgtcttc ctcgatcctg accgcccgat ccgagaggta
     2041 tttgtcaacg tccatgcgcc tgatccggta gttcttgccg acacggatag ccgagagctg
     2101 ccccgacttg atgaggcggt agacggtcat gttggagacc cgcatgatgc cggcgacttc
     2161 ccctaccgtg ag
//
LOCUS       DASQDO010000372         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_222890, whole genome shotgun sequence.
ACCESSION   DASQDO010000372 DASQDO010000000
VERSION     DASQDO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222890"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..1489)
                     /locus_tag="VEV43_10445"
     CDS             complement(359..1489)
                     /locus_tag="VEV43_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23983.1"
                     /translation="MASAGGEWRRSWWIAFTLVPFGWLAFVAFAYAGRRARRRGWISW
                     AILYGVVFWAATVVASVEELDYAVRQSGGLVMFLGTPVPFLHALAIRRAYLDATSGAH
                     VTAPAAVAAPTVRARPPRRWTGIPVFMVIFGVVGLLAGGIQLASAEPVGIAIVAFGGA
                     SILGATLRVPPRRYAAPVAGTVPEGDERGLVFPYAAGRARRTTAGVALFVVLGVSMAV
                     YADEFTSRRRYLARPAGILAAIGATATLAFGLTPRRGGAIALTPARVSIVNGRFRVAV
                     PWESIADVRPATGYGGQPLVAVDAVATRAVEKSAVARWATVFHGRSFGHVTIEPQAAD
                     ADPLALLRVVTHYWRHPDERGDLAAPGVVERFAESASGGSGS"
     gene            complement(1679..>3592)
                     /locus_tag="VEV43_10450"
     CDS             complement(1679..>3592)
                     /locus_tag="VEV43_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYP23984.1"
                     /translation="TFHSACVRILRREAPRLGLRSSFSIYDSADSARLLKLCYKELNV
                     DEKRMPPRAVAAAISDAKNKLVDSGFYQDFASNPWERTVAKLYVEYQRRLNDCSAMDF
                     DDLIFRTVEVFDKFPEALGHYQTRFEHVLVDEFQDTNHAQARLSTLLAGKHRNIFVVG
                     DADQGIYAFRGATIKNLLDFERDWPEAHVITLEQNYRSTQTILNAANAVIENNAMRKP
                     KSLWTESSGGDLITRYHAQNEHDEALWVAREVARIKDLGYALADMAVFYRTNAQSRVL
                     EEMFSTEDVSYRVVGGVRFYERKEVKDLLAWLRAAMNPSDAVSVARAAQAPRRGIGDT
                     TLARVGGFAQVAGIPLGEAFDRAEEVPGLNKRAIGGVLEAARLFERIRTTAESGVPVA
                     EIVETAWEVTGYMDELKSERTFESLSRQENLRELAGVAQEFDEGPNASDGLTGFLEQL
                     ALITDTDTVDGEEAGVTLMTLHNAKGLEFPVVFMIGMEDGVFPHLRSLGDPDQLEEER
                     RLCYVGITRARERLYLLNAWSRSLWGNLNYNPPSRFLSEIPVELVNQANERKPSATSK
                     TVTLPRSRQERELGDPDAYKVGQEVEHAKWGRGTIVEIARSPVGVEATVNFPAAGGFK
                     RLDLSLAPLKPAS"
BASE COUNT          567 a         1266 c         1225 g          534 t
ORIGIN      
        1 cgagagctac gagtacgcga agaagcacaa cccggagtcg aagaagatcc cgaagaagaa
       61 gccgaagaac ttcctgaagg acatgctcga cttctaggcg agacagagcg cggagagggg
      121 gacctcgcgg tcccccctct catccccacg tgctcgacgt aaagtcgaca gccccgcctg
      181 cgctgcagcc cacctagtcg actgcccgca ccgggccatg gttccgagag acggtccgcc
      241 gtcactcccc cgccgcctct tgtcacgtcc cgctgtccca cgctccgacc agggccgcgc
      301 tgacgtagag ggatacgaac ggggggccgc tccccttacg cgggggccgg aaaaatcttc
      361 aagagcccga acccccagag gcgctctcgg cgaagcgctc gacgaccccc ggcgcggcga
      421 ggtccccgcg ctcgtcggga tgccgccagt agtgggtcac gacccgcagc agcgccagcg
      481 ggtcggcgtc cgccgcctgc ggctcgatcg tgacgtgtcc gaacgatctc ccgtggaaca
      541 ccgtcgccca ccgcgcgacg gcggacttct cgacggcccg ggtggcgacc gcgtcgacgg
      601 cgaccagagg ctggcccccg tatccggtag cagggcgcac gtccgcgatc gactcccacg
      661 gcactgcgac tcggaaccgt ccgttgacga tgctgactcg cgccggcgtc agcgcgatcg
      721 cgccgccgcg ccgaggcgtc agaccgaagg cgagggtggc cgtcgcgccg atggcggcga
      781 gaattccggc cggccgtgca aggtaacggc gacgcgacgt gaactcgtcg gcgtagaccg
      841 ccatgctcac gccgaggacg acgaacaacg cgacgccggc cgtcgtacgg cgggctcgcc
      901 cggccgcgta ggggaagacg aggccgcgct cgtcgccctc gggaaccgtc cccgcgacgg
      961 gggcggcgta ccttcggggc gggacgcgca gcgtggcgcc gaggatcgac gcgcctccga
     1021 acgcgacgat cgcgatcccc accggctccg cggatgcgag ctggatgcct cccgccagca
     1081 gccctacgac tccgaagatc accatgaaca cggggatgcc cgtccaccgc cgcgggggcc
     1141 gggcgcgaac ggtgggggcg gccacggctg ccggcgccgt cacgtgagcg ccggacgtcg
     1201 cgtcgaggta cgcgcgccgt atcgcaagcg cgtggaggaa cgggaccggc gttccgagga
     1261 acatcacgag cccgccgctc tgccggacgg cgtagtcgag ctcctcgacg gacgcgacca
     1321 ccgtggccgc ccagaacacg acgccgtaca agatcgccca cgagatccag ccgcgccgtc
     1381 gcgctcgcct acccgcgtag gcgaacgcga cgaacgcgag ccacccgaag ggcacgagcg
     1441 tgaacgcgat ccaccacgac cttctccact ccccgcccgc actcgccacg cagggagctt
     1501 acgaactggc cgggctcagt cggggtctat gacgatcacg cagggatggt tgtaaagcgg
     1561 ctcgggttct ccgccggtca ccaagcgtcc attgctgcct cctctcggag agccatcccg
     1621 tgaggagcac ttagagagga gaggcccggc ccctccccgt cgagaggcct ttcgtgggct
     1681 acgaagcggg cttcaggggg gccagggaga ggtccagccg cttgaagcct ccggccgccg
     1741 ggaagttcac cgtcgcctcc acgcctacgg gcgagcgcgc gatctccacg atcgtcccgc
     1801 ggccccattt cgcgtgctcc acctcttgcc cgaccttgta cgcgtccggg tcgcccagct
     1861 cgcgttcctg gcgggagcgc ggcagcgtca cggtcttcga cgtcgccgag ggcttgcgct
     1921 cgttcgcctg gttgacgagc tcgaccggga tctcggacag gaaccgcgac ggcgggttgt
     1981 agttgaggtt cccccacagg ctgcgcgacc acgcgttcag aagatagaga cgctcgcgtg
     2041 cccgcgtgat gccgacgtag cagagccgcc gctcttcttc gagctggtcg gggtcgccca
     2101 gcgagcgcag gtgggggaac accccgtcct ccatcccgat catgaacacg acggggaact
     2161 cgagtccctt cgcgttgtgc agcgtcatca gcgtgacgcc ggcctcctcg ccgtcgaccg
     2221 tgtcggtgtc ggtgatcagc gcgagctgct cgaggaaccc cgtgagcccg tcgctcgcgt
     2281 tcgggccctc gtcgaactcc tgtgcgacgc cggcgagctc gcgcaggttc tcctggcggg
     2341 agagagactc gaacgtgcgc tcggacttga gctcgtccat gtagccggtg acctcccacg
     2401 ccgtctcgac gatctcggcg accgggacgc cgctctccgc cgtggtgcgg atgcgctcga
     2461 acaggcgagc ggcctcgagc acgccgccga tcgcgcgctt gttgagcccc ggcacctcct
     2521 cggcccggtc gaacgcctca ccgagcggga tccccgcgac ctgtgcgaat cccccgacgc
     2581 gcgcgagcgt cgtgtcgccg atcccgcggc ggggggcctg ggcggcccgc gcgacgctga
     2641 cggcgtccga cgggttcatc gcggcccgca gccacgcgag gaggtctttc acctccttgc
     2701 gctcgtagaa cctcacgccg ccgacgaccc ggtaggacac gtcctccgtg ctgaacatct
     2761 cctcgagcac gcgcgactgc gcgttcgtcc ggtagaacac ggccatgtcg gccagcgcgt
     2821 agccgaggtc cttgatgcgc gccacctcgc gcgcgaccca cagcgcctcg tcgtgctcgt
     2881 tctgcgcgtg gtagcgcgtg atgaggtcgc cgccggacga ctccgtccac agcgacttcg
     2941 gcttgcgcat cgcgttgttc tcgatcaccg cgttggcggc gttgaggatc gtctgcgtcg
     3001 agcggtagtt ctgctcgagc gtgatcacgt gggcctcggg ccagtcccgc tcgaagtcga
     3061 gcaggttctt gatcgtcgcc ccgcggaacg cgtagatgcc ctggtccgcg tcgccgacga
     3121 cgaagatgtt gcggtgcttg ccggcgagca gcgtcgagag ccgggcctgc gcgtggttcg
     3181 tgtcttggaa ctcgtcgacg aggacgtgct cgaagcgtgt ctggtagtgg ccgagggctt
     3241 cggggaactt gtcgaagacc tcgacggtcc tgaagatgag gtcgtcgaag tccatcgccg
     3301 agcagtcgtt cagcctccgc tggtactcga cgtacagctt cgcgaccgtg cgctcccacg
     3361 ggttcgaagc gaagtcctgg tagaaacccg agtcgacgag cttgttcttg gcgtcggaga
     3421 tcgcggccgc gaccgcgcgg ggcggcatcc gcttctcgtc gacgttcagc tccttgtagc
     3481 agagcttcag aaggcgggcg gagtcggcgg agtcgtagat cgagaacgac gagcgaaggc
     3541 cgagccgggg cgcctcacgc cggaggatgc gcacgcacgc ggagtggaac gt
//
LOCUS       DASQDO010000373         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1018347, whole genome shotgun sequence.
ACCESSION   DASQDO010000373 DASQDO010000000
VERSION     DASQDO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018347"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VEV43_10455"
     CDS             complement(<1..945)
                     /locus_tag="VEV43_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYP23985.1"
                     /translation="MTIYENLLDGLGNTPLMRLNRIGKGLACEFVGKLELLNPGGSVK
                     DRIGIRMIEEAERTGKLRPGGTIVEPTSGNTGVGLAIAAAIKGYRCIFTMPDKMSQEK
                     ISILRAYGAEVVITPTAVAPDSPESYYRVADRLTEEIPGAFQPNQYHNQSNPEAHYVT
                     TGPELWEQTEGRITHLVVSVGTGGTVTGSGRYLKEKNPDVQLVGADPDGSIFSGGEPH
                     PYLVEGIGKEAWPDTLDRSVVDRWIKVGDRESFLTARRITREEGILVGGSSGTAVWAA
                     LEVAKDLGPDDFVVVILPDSGKSYLSKIYNEGWMLDHGF"
     gene            1083..2300
                     /locus_tag="VEV43_10460"
     CDS             1083..2300
                     /locus_tag="VEV43_10460"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYP23986.1"
                     /translation="MIGAKKRFRMPHLGRRWTIALVTVLGLVFLGAAGAAYATYDYSN
                     EYDGRMLPGTMIAGVDVGGMDRDEALAAVRDAVRPEIRRTISVEWDDETWNVTPHKIG
                     AFTTAGRAIDQALAASAETSFFQKMRMRVLGAELEHTGDVAVRYPAKGARAFLEGIAS
                     SVNRDVEDAELDYSSGWVKFVDGKDGRRVNVADSHKAFMKALRSGDDSSELKVKVTKP
                     EKTTEDFDQVLLVRIGENKLYLYEDQKITHEWSVATGQPEYMTPTGLFEVELKRYMPT
                     WVNPAPDTWGANMPATIPPGPGNPLGVRAINWTAPAIRFHGTSATYSLGYNASHGCVR
                     MANEDVIQLYDMIDVGTPIVSVVAGPLKPMYSSSSFDEPEPVAPEAENEATQSNAGDA
                     SKPGSKKPSKGDG"
     gene            2373..>3079
                     /locus_tag="VEV43_10465"
     CDS             2373..>3079
                     /locus_tag="VEV43_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HYP23987.1"
                     /translation="MVPELLELQRLQSDPPAHDELLFITVHQVYELWFKQLLFELESI
                     RDLMAANKPSGARHLLERVMTIEEVAITQIRVLETMAPKDFLEFRQHLAPASGFQSVQ
                     FREIEFISGLKDARYLDRVSESPEERARLEKRLAEPTLWDAFCTMLDQRGLPMPPDDV
                     EARRTSLLEVARDRESYPDEYYLSEALLEHDQHFATWRSRHVLMVERQIGTKSGTGGS
                     TGAPYLRSTLNKRFYPEL"
BASE COUNT          507 a         1083 c         1023 g          466 t
ORIGIN      
        1 gaagccgtgg tcgagcatcc agccctcgtt gtagatcttc gacaggtagc tcttcccgga
       61 gtccgggagg atcacgacga cgaagtcgtc ggggccgagg tccttcgcga cctcgagcgc
      121 ggcccacacg gcggtccccg acgagccgcc gaccaggatg ccctcctccc gcgtgatccg
      181 ccgcgccgtc aggaacgact cgcggtcgcc gaccttgatc caccggtcga ccacggaccg
      241 gtcgagcgtg tccggccagg cctccttgcc gatcccctcg acgaggtacg ggtggggctc
      301 gccgcccgag aagatcgacc cgtccgggtc ggccccgacg agctggacgt ccgggttctt
      361 ctccttcagg tagcggccgc tccccgtcac ggtgccgccg gtcccgacgc tgacgacgag
      421 gtgcgtgatc cggccctcgg tctgctccca cagctccggc cccgtcgtca cgtagtgcgc
      481 ctccgggttc gactggttgt ggtactggtt cggctggaag gcgcccggga tctcctcggt
      541 gagccggtcg gcgacccggt agtaggactc cggcgaatcc ggcgccaccg cggtcggcgt
      601 gatgacgacc tcggccccgt aggcgcgcag gatcgagatc ttctcctggc tcatcttgtc
      661 gggcatcgtg aagatgcagc ggtagccctt gatcgccgcg gcgatcgcca gccccacgcc
      721 ggtgttgccc gaggtgggct cgacgatcgt cccgccgggc cggagctttc cggtcctctc
      781 ggcctcctcg atcatccgga tcccgatgcg gtccttcacg ctgccgccgg ggttgaggag
      841 ctcgagcttc ccgacgaact cgcaggccag gcccttgccg atgcggttga gccgcatcag
      901 cggcgtgttg ccgaggccgt cgaggaggtt ctcgtagatc gtcatgtgac ccatcgtgtc
      961 agcccggcgt cgaaaggtgc aagcacggaa cattgggcgg gtttgctcgg ttctctttcg
     1021 cgatctcgcg agacacctat acgatgggcg gccttgtgga aaacgtggaa aacgaaaaag
     1081 acgtgatagg ggccaagaag cgcttccgga tgccgcacct cggccgccgc tggacgatcg
     1141 cgctcgtgac cgtcctcggc ctcgtgttcc tcggggccgc cggcgccgcc tacgcgacct
     1201 acgactactc gaacgagtac gacggccgga tgctccccgg caccatgatc gccggcgtcg
     1261 acgtcggcgg gatggaccgc gacgaggcgc tggccgccgt ccgtgacgcc gtccggcccg
     1321 agatccgccg gacgatctcc gtcgagtggg acgacgagac ctggaacgtc acgccgcaca
     1381 agatcggggc cttcacgacc gccggccggg cgatcgacca ggccctcgcg gcgagcgcgg
     1441 agacctcctt cttccagaag atgcggatgc gcgtgctggg ggccgagctc gagcacaccg
     1501 gcgacgtcgc ggtccgctac ccggcgaagg gcgcccgcgc cttcctcgag ggcatcgcgt
     1561 cctcggtcaa ccgcgacgtc gaggacgccg agctcgacta ctcgagcggc tgggtgaagt
     1621 tcgtcgacgg caaggacggc cgccgcgtga acgtcgccga cagccacaag gccttcatga
     1681 aggcgctgcg ctccggcgac gactcgagcg agctgaaggt caaggtcacc aagccggaga
     1741 agacgaccga ggacttcgac caggtcctgc tcgtccgcat cggcgagaac aagctctacc
     1801 tctacgagga ccagaagatc acgcacgagt ggagcgtcgc gacgggccag cccgagtaca
     1861 tgacgccgac cggtctgttc gaggtcgagc tcaagcgcta catgccgacg tgggtcaacc
     1921 cggcccccga cacctggggc gcgaacatgc cggcgacgat cccacccggc ccgggcaacc
     1981 cgctgggcgt gcgcgccatc aactggaccg caccggcgat ccgcttccac gggacgagcg
     2041 cgacgtactc gctcggctac aacgcgagcc acggctgcgt ccgcatggcg aacgaggacg
     2101 tgatccagct ctacgacatg atcgacgtcg gcaccccgat cgtctcggtg gtggccgggc
     2161 cgctcaagcc gatgtactcg tcgtcgtcgt tcgacgagcc cgagcccgtc gcgccggagg
     2221 ccgagaacga ggccacgcag agcaacgccg gcgacgcgtc gaagcccggc tcgaagaagc
     2281 cctccaaggg agacgggtag ctgggggaca acccccgccg gcccccccgc ttcggcgagg
     2341 aggaccggcg cctctcctac ggcagctacc tgatggtccc ggagctcctc gagctccagc
     2401 gtctccagtc cgacccaccg gctcacgacg agctgctgtt catcaccgtc caccaggtct
     2461 acgagctctg gttcaagcag ctcctcttcg agctcgagtc catccgcgac ctgatggcgg
     2521 ccaacaagcc gtcgggggcc cggcacctcc tcgaacgcgt gatgacgatc gaagaggtcg
     2581 cgatcacgca gatcagggtg ctggagacga tggccccgaa ggacttcctc gagttccgcc
     2641 agcacctcgc ccccgccagc gggttccaat ccgtccagtt ccgtgagatc gagttcatct
     2701 ccggcctcaa ggacgcgcgc tacctcgacc gggtgtcgga gagcccggag gagcgggccc
     2761 gcctcgagaa gaggctcgcc gagccgactc tgtgggacgc gttctgcacg atgctcgacc
     2821 agcgcgggct gccgatgccg ccggacgacg tcgaggcccg gcgcacgtcc ctgctcgagg
     2881 tcgcccgcga ccgtgagagt tatcccgacg agtactacct gtccgaggcg ctgctcgagc
     2941 acgaccagca cttcgccacg tggcgaagcc ggcacgtgct gatggtcgag cgacagatag
     3001 ggacgaagag cgggacgggc ggctccacgg gggccccgta cctgcggtcg acgctgaaca
     3061 agcgcttcta tccggagct
//
LOCUS       DASQDO010000374         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_712753, whole genome shotgun sequence.
ACCESSION   DASQDO010000374 DASQDO010000000
VERSION     DASQDO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712753"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..614)
                     /locus_tag="VEV43_10470"
     CDS             complement(111..614)
                     /locus_tag="VEV43_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP23988.1"
                     /translation="MADDALVEFCRVQYPRLVGFLGLYCGNRAVAEELAQETLARVWR
                     KWPKVHHLDRPDAWAQRVAINLANSHFRRLVAERKARARAGEIAEPHNPAVEDALAVR
                     RAVAALPRRQRAAVVLHYFLDLPLAEVAERMETTVPAVKALLHRAVRRLRNEGEVVDP
                     KEVPDVV"
     gene            complement(674..1039)
                     /locus_tag="VEV43_10475"
     CDS             complement(674..1039)
                     /locus_tag="VEV43_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP23989.1"
                     /translation="MPEEPQTDAWVRKAFELELDATKTLITLSSAILALTITLGTDVK
                     QHIELLGATWIAFLISLLGGVWELRGLGKELERAATDAESPTLRAFGIIVPSRLQNAA
                     FGVGTALLVYYGLVTIRSA"
     gene            1119..1676
                     /locus_tag="VEV43_10480"
     CDS             1119..1676
                     /locus_tag="VEV43_10480"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYP23990.1"
                     /translation="MKIYTKKGDDGTTGLLYGGRVHKDDPRTEVYGTLDETVSALGVA
                     RAGGLTPRVEEIVVRVQREMFVAGAQLATAEENQPKLQDGVSRVTPAMTERAEHDIDA
                     LLDEHPLPQEFVLPGETPGSAGLDLARSVVRRAERQAVAMNGRGQVADPEVLRYLNRV
                     SDLLFALARYEEASRGLRAAPSRER"
     gene            1682..2212
                     /locus_tag="VEV43_10485"
     CDS             1682..2212
                     /locus_tag="VEV43_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYP23991.1"
                     /translation="MPSVSPEPEPFRFCPVDGTALDDPDKEGGSSCGVCGRSWYRNSS
                     PVVGCVIVRDGKALVTVRGVEPFKGKLDAPGGFLLNGEDPIEGLKREIEEELGIQIEV
                     GVEDCLLMAPHVYAHENDFNLALGFRARWVSGEPSPADDVADLRWVTRAEVETLEFAW
                     EHDRELVRKALDHEEG"
BASE COUNT          344 a          826 c          846 g          336 t
ORIGIN      
        1 ccccggcggc gacgcggacc agcgtgatcc gacgggcccg ccgctcgatc ctgcgctcgt
       61 cgaaccccgc cgcaggacgg ggagctgctt cgtgcaacag cgtgcggagg tcagacgaca
      121 tccggcacct ccttggggtc gacgacctcg ccctcgttcc gaaggcgccg gaccgcgcgg
      181 tgcagcaggg ctttgacggc gggcaccgtg gtctccatcc gttcggcgac ctccgcgagc
      241 ggcaggtcga ggaagtaatg gaggacgacc gcggccctct ggcggcgcgg gagggccgcg
      301 acggcccggc ggaccgcgag agcgtcctcg acggcggggt tgtgcggctc ggcgatctcc
      361 ccggctcgcg ctcgtgcctt gcgctccgcg acgaggcgcc ggaagtgact gttggcgagg
      421 ttgatcgcca cgcgctgcgc ccacgcgtcc gggcggtcca ggtgatggac cttcggccac
      481 ttgcgccaca cccgcgcgag ggtctcctgc gccagctcct cggccaccgc ccggttcccg
      541 cagtagagcc cgaggaagcc gacgagccgc gggtactgca cccggcagaa ctccaccagc
      601 gcgtcgtcag ccatgcgtcc gcctcatgcc cgtcacacgc acgagccggc gccgtggtcg
      661 cgcgcctcgg ggctcaggcc gatctgatcg tgaccaggcc gtagtagacg agcagcgcgg
      721 tcccgacccc gaacgcggcg ttctggagtc gcgacgggac gatgatcccg aaagcccgca
      781 gcgtcgggct ctcggcgtcc gtcgcggcgc gctcgagctc cttcccgagc ccgcgaagct
      841 cccagacgcc acccaggagc gagatcagga acgcgatcca cgtcgcgccc agcagctcga
      901 tgtgctgctt cacgtcggtg cccagcgtga tcgtcagcgc gaggatggcg gacgaaagag
      961 tgatcagcgt cttggtcgcg tcgagctcga gctcgaaagc ctttcggacc cacgcatccg
     1021 tctggggctc ctcgggcacc agtccccccc gtccgcgtcg cctggcgcgc tgaacaagac
     1081 gataccgccg tgggtcgcca cggataggct cgccccccgt gaagatctat acgaagaagg
     1141 gcgacgacgg caccaccggg ctcctctacg ggggccgcgt ccacaaggac gaccctcgga
     1201 ccgaggtgta cgggaccctc gacgagaccg tgtcggccct cggcgtcgcc cgagcgggtg
     1261 ggctgacgcc gcgggtcgag gagatcgtcg tccgggtgca gcgggagatg ttcgtcgccg
     1321 gcgctcagct cgccaccgcc gaggagaacc agccgaagct ccaggacggc gtgtcgcggg
     1381 tcacccccgc catgaccgag agggccgagc acgacatcga cgcgctcctc gacgagcacc
     1441 cgttgccgca ggagttcgtc cttcccggag agacccccgg gtcggccggt ctcgacctcg
     1501 cgcgctcggt cgtccggcgc gccgagcgcc aggccgtcgc gatgaacggc cggggccagg
     1561 tcgccgaccc cgaggtgctc cgctacctca accgcgtgtc cgacctgctg ttcgccctcg
     1621 cccgttacga ggaggcgtcg cgcgggctcc gcgcggcccc ctcgcgcgag cggtgacgta
     1681 gatgccgagc gtgtctcccg agccggagcc cttccgcttc tgccccgtcg acgggaccgc
     1741 cctcgacgac cccgacaagg agggcggctc gagttgcggc gtctgcgggc gctcctggta
     1801 ccggaactcg tcgccggtcg tcggctgcgt gatcgtgcgc gacggcaagg cgctcgttac
     1861 cgtgcgcggg gtcgagccgt tcaagggcaa gctcgacgct cccggtggct tcctcctgaa
     1921 cggcgaggac ccgatcgagg gactcaagcg ggagatcgag gaggagctcg gcatccagat
     1981 cgaggtgggg gtcgaggact gtctgctgat ggccccccat gtgtacgcgc acgagaacga
     2041 cttcaacctc gcgctcgggt tccgggcccg ctgggtgtcg ggcgagccgt cgccggcgga
     2101 cgacgtggcg gacctgcgct gggtgacgcg cgccgaggtg gagactctgg agttcgcgtg
     2161 ggagcacgac agggagctcg ttagaaaggc tttggaccat gaagaaggat gagatcagcg
     2221 tcttggggga cctggacgag gaggacctgg cgcgtctgta cggcgtgacc cgcctgacgc
     2281 tcggcatcgc gacgttcgtc gccccctcgc tcgttgcgaa ggtgtggatg gggcgtgacg
     2341 ccgcggacgc gg
//
LOCUS       DASQDO010000375         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1202416, whole genome shotgun sequence.
ACCESSION   DASQDO010000375 DASQDO010000000
VERSION     DASQDO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202416"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..715)
                     /locus_tag="VEV43_10490"
     CDS             complement(2..715)
                     /locus_tag="VEV43_10490"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HYP23992.1"
                     /translation="MTVQILTNSQINKAGKALREWWDDFEAPVEALDTYLDDAFHVLV
                     RFRAAHQRPMTKATRGLRMMVATEGAKVEVSQRLKRIPTIVDKLSRQPSMKLANMHDI
                     AGCRAVLQTVDEVRRVERRLSRNRPPVRVYDYIEGPRESGYRGVHVIVLYDNRKIEVQ
                     LRTRVMHDWAIAVERLGGRLQTDLKSGRGPAPLLAWLSAVSEAMALEEAGEVVDTQLV
                     ERIQVLRKGALPYLQEGRP"
     gene            complement(813..1289)
                     /locus_tag="VEV43_10495"
     CDS             complement(813..1289)
                     /locus_tag="VEV43_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HYP23993.1"
                     /translation="MRKALASLVALGLVASFAASPAVAAKGKKKKVTKTISETIDVTA
                     LPFPNYSSRTGTALPGCTAGEEGVHKHTHPFETPGAGTLTLAMSGFTGDWDLYVFNSD
                     GVAIARSDQEQAGPAMAPAEEKVVMPMGKGESYELVICNWAGAPQASASLEYVYTV"
     gene            complement(1380..2537)
                     /locus_tag="VEV43_10500"
     CDS             complement(1380..2537)
                     /locus_tag="VEV43_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP23994.1"
                     /translation="MKILVLGGDGYLGWPTAMHLSAAGHDVALVDNFLRRAMSTELGA
                     ESLTPIQSIGERIRAWKEVSGNDLEFFHGDLADPQFVDEVFTRFRPDGIVHYGEQPSA
                     PYSMIDRRHAVATQRNNVEGTLNVLYAMRDLAPEAHLVKLGTMGEYGTPNIDIEEGFI
                     EINHNGRTDTLPFPKLPGSMYHLTKVHDSHNIHFGCRIWKLRSTDLNQGVVYGIHTAE
                     SQLDERLCTRFDYDEVLGTALNRFCVQAVVGYPLTVYGKGGQTRGFLNIVDTIQCVRL
                     ALENPAAPGEYRVFNQFTEQFSVNDLASIVARVGASKGINVVVDPVADPRIEAEEHYY
                     NATHTKLVELGLKAHLLTDEVVGDLLDTIQRYKDRILLGPIAPKTKWDPRA"
     gene            2559..4520
                     /gene="acs"
                     /locus_tag="VEV43_10505"
     CDS             2559..4520
                     /gene="acs"
                     /locus_tag="VEV43_10505"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HYP23995.1"
                     /translation="MATEKTIEALLEERRTFEPPEDFAAAAVFGDAAVYSEAERDPDA
                     WWASQAERLDWFERWESVMEGGFPHHTWFRGGRLNASHNCLDRHLASHGDRVAYHWIG
                     EPGQTRTITYRDLHEEVCRLANVLKDLGVTKGDRVAIYLPMVPELPASMLACARIGAV
                     HSVVFGGFSAESLRDRINDSECKVLVTADGGYRRGAVVPLKANADEAVEGTPSISKVI
                     VVKRTDGDVPMVEGRDVVYADVVAAASADCPPESMDAEDPLYILYTSGTTGKPKGIVH
                     TTGGYLTGVSSTHRAIFDLKPETDVYWCAADIGWVTGHSYIVYGPLCNGTTSILYEGA
                     PDWPDKDRWWSIVEEYRATILYTAPTAIRTFMKWGTGYPAKHDLSSLRLLGSVGEPIN
                     PEAWVWYQEFVGGGRTPVVDTWWQTETGAIAISALPGITTLKPGSATRPYPGVVADVV
                     DEKGEPVGRGGGGYLVLKRPLPSMFRTIWGDEQRYLDTYFSRFGEDVYFPGDGAQLAD
                     DGYFWLLGRVDDVMNVSGHRISTYEVESALVDHQGVAEAAVVGRQDPREGETIVAFVT
                     PKGGVEGDEALLTELRDHVAKVIGKIARPQSIVFTDDLPKTRSGKIMRRLLRDVAEGR
                     ALGDVTTLQNAPILDEIRAKADSLKGDEA"
     gene            complement(4517..5374)
                     /locus_tag="VEV43_10510"
     CDS             complement(4517..5374)
                     /locus_tag="VEV43_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP23996.1"
                     /translation="MMRAGWRVVGAFLVAAGAAALPGHGLAAGVCSPDPTPGDDEISC
                     SEEADSLSGLAGDDVLFGGSGTDLLGGDAGNDQVYGEAGDDSLSGDPGSDYLEGETGR
                     DTLDGGEGSDDLRGGTGVDTIFGGPGDDAMDGGAHEDDVQGHAGNDLIFGWHGDDSLS
                     GGPGEDDLSGFIGADSIRGGSGLDVLYGGDGSDRILGGGGGDFVQGSAGSDHLDGGNG
                     RDFLVGGKSKDTIYALSGKDKVKAGDGDDEVFAADGVVDRISCGPGADVVHVDEFDVL
                     VDCEEVDAT"
     gene            5502..>6230
                     /locus_tag="VEV43_10515"
     CDS             5502..>6230
                     /locus_tag="VEV43_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HYP23997.1"
                     /translation="MSDVAIGSGAGTAPPAPAEEGQASLARRLFAEFTGTTVFVAIGT
                     GAATILGIGPIRSLAAIPAEALGGEIGEGLFGSLLGSTFSDVLGVAFAFATALAVLVY
                     AFGGVSGAHFNPAVTISLAVARRFRWKDVPAYVVVQCLGAIAGAFIVAGIYGQTGAAL
                     EGQDIFFGATTVSDDIDFVNGLLAEAFIGFILVTGIMAIAVDPRAPKGWSGLVIGLAL
                     AAGILVTADATGGSANFARSLGPFV"
BASE COUNT          962 a         2137 c         2113 g         1018 t
ORIGIN      
        1 gtcatgggcg gccctcctgc aaatacggta gcgcaccttt tcggagtacc tggattcgct
       61 cgactagctg agtatcgacc acttccccgg cctcctcaag agccatcgcc tcggagacgg
      121 cactcagcca agcgaggagc ggggccgggc ccctcccgct cttcaggtcc gtttgcaagc
      181 ggcccccgag acgttcgact gcaatagccc agtcgtgcat gacacgcgtc cgcaattgca
      241 cctcgatctt ccgattgtcg taaaggacga ttacatggac ccctctatat cccgactccc
      301 gcggtccctc aatgtagtcg tagacgcgaa ccgggggccg gttccgcgaa agccggcgct
      361 caactcggcg aacctcgtca acggtctgca ggaccgcccg acacccagcg atgtcgtgca
      421 tgttcgccag cttcatggac ggctgccgag agagcttgtc gacgatggtc gggatgcgct
      481 tgagtcgttg agaaacctcg accttcgctc cttcagtcgc gaccatcatc cgcaaacctc
      541 tcgtcgcctt cgtcatcggt cgctgatgag ccgcccgaaa ccggacgagg acgtggaatg
      601 cgtcatcgag gtaggtatcc agagcttcga caggggcctc gaagtcatcc caccactcgc
      661 gtagcgcctt gcccgccttg ttgatctgcg agttcgtcag aatctgaacg gtcaccacac
      721 gaccatatgg cgatcaatgg acaaccttcg gtcatttccc gggacgcgaa agaggggccc
      781 cggggggccc ctcttgttcg aagctgtcga agctagaccg tgtacacgta ctccagcgag
      841 gcggacgcct gcggggcgcc ggcccagttg cagatgacca gctcgtagct ctcacccttg
      901 cccatcggca tgacgacctt ctcctcggcc ggggccatcg ccgggccggc ctgctcctgg
      961 tccgagcgag cgatcgcgac gccgtcggag ttgaagacgt acaggtccca gtcaccggtg
     1021 aagccggaca tcgccagcgt cagggtgccg gcgccggggg tctcgaacgg gtgggtgtgc
     1081 ttgtggacgc cctcttcacc ggcggtgcag cccggaagcg cggtcccggt gcggctcgag
     1141 tagttcggga acggaagggc ggtcacgtcg atggtctcgg agatcgtctt cgtgaccttc
     1201 ttcttcttgc ccttggccgc gacggcgggg cttgccgcga agctggccac gagcccgaga
     1261 gctacgagcg aagcaagtgc cttgcgcatg acgtcatctc ctgggtttgg ggtctctgag
     1321 cccaggattc gccgccccgg cgagcactcc tgcggcggca agtacgcggt taaggccggt
     1381 caggcccggg ggtcccactt cgtcttcggc gcgatcggcc ccagcaggat ccggtccttg
     1441 taccgctgga tcgtgtcgag gaggtccccg acgacctcgt cggtgagcag gtgcgccttc
     1501 aggccgagct cgaccagctt cgtgtgggtc gcgttgtagt agtgctcctc ggcctcgatc
     1561 ctcgggtccg ccaccgggtc gacgacgacg ttgatgccct tcgaggcacc gacgcgggcg
     1621 acgatcgaag cgaggtcgtt gaccgagaac tgctccgtga actgattgaa cacgcggtac
     1681 tcgccggggg ccgcggggtt ctcgagcgcg agccggacgc actggatcgt gtcgacgatg
     1741 ttgaggaacc cgcgcgtctg gccccccttg ccgtagaccg tcagcgggta cccgacgacg
     1801 gcctgcacgc agaagcggtt gagcgcggtc cccaggacct cgtcgtagtc gaagcgcgtg
     1861 cacagacgtt cgtcgagctg cgactccgcc gtgtggatcc cgtagacgac gccctggttg
     1921 aggtcggtcg agcgcagctt ccagatgcgg cagccgaagt ggatgttgtg cgagtcgtgg
     1981 accttcgtca ggtggtacat cgagcccggc agcttcggga acggcagcgt gtcggtgcgc
     2041 ccgttgtggt ttatctcgat gaagccctct tcgatgtcga tgttcggcgt gccgtactcg
     2101 cccatcgtcc cgagcttcac gaggtgcgcc tcgggcgcga ggtcgcgcat cgcgtagagg
     2161 acgttgagcg tcccctctac gttgttgcgc tgcgtcgcga ccgcgtgacg ccggtcgatc
     2221 atcgagtagg gagcgctggg ctgctctccg tagtgaacga tcccgtcggg ccggaaccgt
     2281 gtgaagacct cgtcgacgaa ctgcgggtcc gcgaggtcgc cgtggaagaa ctcgaggtcg
     2341 ttgccggaga cctccttcca ggcgcggatg cgctcgccga tcgactggat cggcgtcagc
     2401 gactcggccc ccagctcggt cgacatcgcg cgtcgaagga agttgtcgac gagcgcgacg
     2461 tcgtggcccg cggccgagag atgcatggcg gtcggccacc cgaggtagcc gtccccgccc
     2521 aggacgagga tcttcacgcc cggcaggata atggcccgat ggcgaccgag aagacgatcg
     2581 aggcgctgct cgaggaacga cgtacgttcg agccccccga ggacttcgcc gccgctgcgg
     2641 tcttcggcga cgccgccgtc tactccgagg ccgagcgcga ccccgacgcg tggtgggcgt
     2701 cccaggccga gcgactcgac tggttcgagc gctgggaatc cgtgatggag ggcgggttcc
     2761 cccaccacac ctggttccgg ggcgggcggc tcaacgcgtc gcacaactgc ctcgaccgcc
     2821 acctcgcgtc gcacggggac cgggtcgcct accactggat cggcgagccg ggccagacgc
     2881 gcacaatcac ctatcgcgac cttcacgaag aggtgtgcag gcttgcgaac gtgctcaagg
     2941 acctcggcgt caccaagggc gaccgcgtcg cgatctacct gccgatggtc ccggagctcc
     3001 ccgcatccat gctcgcctgc gcgcgcatcg gcgccgtgca ctcggtcgtg ttcgggggct
     3061 tctccgccga gtcgttgcgc gaccggatca acgactccga gtgcaaggtc ctcgtgacgg
     3121 ccgacggcgg ttaccggcgt ggcgcggtcg tgccgttgaa ggcgaacgcg gacgaggccg
     3181 tagagggcac gccttcgatc tcgaaggtga tcgtcgtcaa gcgcaccgac ggggacgtcc
     3241 cgatggtcga gggccgcgac gtcgtgtacg cggacgtcgt ggcggcggct tcggccgact
     3301 gccctcccga gagtatggac gccgaggatc ccctctacat cctctacacg tccgggacga
     3361 cggggaagcc gaagggcatc gtgcacacga ccggcggata cctcaccggc gtctcgtcga
     3421 cgcaccgcgc gatcttcgac ctgaagcccg agacggacgt gtactggtgc gcggccgaca
     3481 tcggctgggt caccgggcac agctacatcg tgtacggccc cctctgcaac ggcactacct
     3541 cgatcctcta cgagggggcg ccggactggc cggacaagga ccgctggtgg tcgatcgtcg
     3601 aggagtaccg cgcgacgatc ctctacacgg cccccaccgc gatccgcacg ttcatgaagt
     3661 ggggcacggg ctatcccgcg aagcacgacc tctcctccct gcgcctgctg ggctcggtcg
     3721 gcgagccgat caatcccgag gcgtgggtct ggtaccagga gttcgtcggc gggggccgga
     3781 cgccggtcgt cgacacgtgg tggcagaccg agaccggcgc gatcgcgatc tcggcgctgc
     3841 cggggatcac gacgctcaag cccggctccg cgacgcggcc ctacccgggt gtcgtcgcgg
     3901 acgtcgtcga cgagaagggc gagccggtgg ggcggggcgg aggtggctac ctcgtgctga
     3961 agagaccgct gccgtcgatg ttccgcacga tctggggcga cgagcagcgt tacctcgaca
     4021 cgtacttctc gcgcttcgga gaagacgtct acttccccgg ggacggggcg cagctcgccg
     4081 acgacggcta cttctggctg ctgggccgcg tcgacgacgt gatgaacgtg tcggggcacc
     4141 gcatctcgac gtacgaggtc gagtccgcgc tcgtcgacca ccagggcgtc gccgaagccg
     4201 ccgtcgtcgg gagacaggac ccgcgcgagg gcgagacgat cgtcgcgttc gtcacgccga
     4261 aggggggcgt cgaaggtgac gaggcgctgc tgaccgagct gcgcgaccac gtggcgaagg
     4321 tcatcggcaa gatcgcgcgg ccccagtcga tcgtgttcac cgacgacctg ccgaagacgc
     4381 gctcgggcaa gatcatgcgc aggctgctgc gcgacgtagc cgagggccgc gccctgggcg
     4441 acgtgacgac gttgcagaac gcgccgatcc tcgacgagat ccgcgcgaag gccgactcgc
     4501 tgaaaggcga cgaagcctag gtcgcgtcga cctcttcgca gtcgaccagg acgtcgaact
     4561 cgtcgacgtg cacgacgtcg gcccccggcc cgcacgagat gcggtcgacc accccgtcgg
     4621 ccgcgaagac ctcgtcgtcg ccgtctccgg ccttgacctt gtccttgccc gacagcgcgt
     4681 agatcgtgtc cttcgacttg ccgccgacga ggaagtcgcg cccgttcccg ccgtccaggt
     4741 ggtcggaacc cgcggagccc tggacgaagt cgcctccgcc ccctccgagg atccggtccg
     4801 agccgtcgcc tccgtagagg acgtccagcc cggacccgcc gcggatcgag tcggctccga
     4861 tgaaccccga gaggtcgtcc tcgcccggcc cccccgacag cgagtcgtca ccgtgccatc
     4921 cgaagatcag gtcgttgccg gcgtgcccct gcacgtcgtc ttcgtgcgcg ccgccgtcca
     4981 tcgcgtcgtc gccgggaccg ccgaagatcg tgtcgacccc ggtgccgccc cggaggtcgt
     5041 ccgacccctc gccgccgtcc agcgtgtccc gcccggtctc gccctcgagg tagtccgagc
     5101 ccggatcgcc cgagagcgag tcgtcgccgg cctcgccgta gacctggtcg ttcccggcgt
     5161 cgcctcccag gaggtcggtc ccggaccctc cgaacaggac gtcgtcgcct gcgaggccgg
     5221 acagggagtc cgcttcctcc gagcacgaga tctcgtcgtc tccgggcgtc gggtcggggg
     5281 agcagacgcc tgcagcgagc ccgtgcccgg gcaaggcagc cgcgcccgcg gccacgagga
     5341 acgcgcccac gacacgccaa cccgcccgca tcatggcgac gatgatccca gaccgcccgg
     5401 cggcgcgacg gcaaaccgcc ggatcggcgc cgtcagggtg tttgattggc cgagcaccga
     5461 gcattactcg aacggccaac ggagacgacg aaaggagccg catgagcgac gttgcgatcg
     5521 gctccggcgc agggacagca cctcccgcgc cggcagagga aggccaggcg tccctggcgc
     5581 gacggctctt cgccgagttc accggcacga cggtgttcgt cgcgatcggg acgggggccg
     5641 cgacgatcct cgggatcggg cccatccgga gcctcgccgc gatccccgcg gaagccctcg
     5701 gcggcgagat cggcgagggg ctgttcgggt cgctgctggg ctccacgttc agcgacgtgc
     5761 tcggcgtcgc gttcgccttc gcgaccgcgc tcgcggtcct cgtctacgcg ttcggcggcg
     5821 tctcgggcgc tcacttcaac cccgcggtca cgatctcgct cgcggtggcg cggcgcttca
     5881 ggtggaagga cgtccccgcg tacgtcgtcg tccagtgcct cggcgcgatc gccggcgcgt
     5941 tcatcgtggc cgggatctac gggcagaccg gagcggccct cgaaggacag gacatcttct
     6001 tcggcgccac gacggtctcg gacgacatcg acttcgtgaa tggcttgctc gccgaggcct
     6061 tcatcggctt catcctggtg acggggatca tggcgatcgc cgtcgacccg cgcgccccga
     6121 aggggtggtc cggcctggtg atcgggctcg cgctcgcagc gggcatcctc gtcacggctg
     6181 acgcgaccgg tggctcggcg aacttcgcac ggagcctcgg ccccttcgtc
//
LOCUS       DASQDO010000376         6587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_345559, whole genome shotgun sequence.
ACCESSION   DASQDO010000376 DASQDO010000000
VERSION     DASQDO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345559"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VEV43_10520"
     CDS             <1..519
                     /locus_tag="VEV43_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP23998.1"
                     /translation="LSWGPLPVLVPLFVEQELGEGATALGFVFSGYGLGGLAGTVFAG
                     TKGMRFDSVVPAYLGWGLGTVAIGALAFAPSAVAAAALMAVVGFTGQIAEVTWATLLQ
                     KFVPRSLLGRVVSTDWLVSLSLQPLGVALAVPVAAAIGIPAAFAGGAALVLAAMALGL
                     WWRPVREIPEPV"
     gene            complement(516..1712)
                     /locus_tag="VEV43_10525"
     CDS             complement(516..1712)
                     /locus_tag="VEV43_10525"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HYP23999.1"
                     /translation="MASSVVVSAARTAIGKFGGALANVPAVDLGGRAIEAALQRAGSS
                     GDQVDYVIMGQVLQAGTGQITARQAAIKAGIPDTVPALTINKVCLSGLNAIALADQLI
                     RAGEVDVVVAGGMESMDQAPYLLPKARNGYRMGDGKLVDSMIFDGLWDAFTDKHMGDQ
                     SEDVNMEFEVSREAQDEWSLRSHQRAAAAISEGRFDEEIVPFEIPQRKGDPVQFATDE
                     GVRAQTTSETLAKLRPAFRTDGTITAGNASQISDGGAAVVVMSAERAEALGITPMAEI
                     VAHGMSADRPPYLHTVPALAVQAALKKAGLSANDLDLVEINEAFAAVAIHSTRMLFNG
                     DKSAEDKVNVNGGAVALGHPIGCTGARLTVTLLHELKRRGGGLGAAALCGGGGQGDAL
                     IFRVAG"
     gene            1772..2548
                     /locus_tag="VEV43_10530"
     CDS             1772..2548
                     /locus_tag="VEV43_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HYP24000.1"
                     /translation="MPTLRTERRDRTLVVTIDRPQVRNAVDRSTADALSAAFGAFEAD
                     DEADVAVLTGAGGHFCAGADLKAMGEDPDRANRVEPEGDGPMGPTRMLLRKPVIAAIE
                     GYAVAGGLELALWCDLRVAAEDAVLGVFSRRFGVPLIDGGNVRLPRVVGLGRALDMIL
                     TARAVDAPEALSIGLVNRVCAPGAALETALDLAQTIASWPQGPLRGDRLAAIEASSLP
                     LDDAMRNEFHRGVESLSAGQTLPNVRRFAAGEGRHGEGTR"
     gene            2568..3008
                     /gene="mce"
                     /locus_tag="VEV43_10535"
     CDS             2568..3008
                     /gene="mce"
                     /locus_tag="VEV43_10535"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HYP24001.1"
                     /translation="MLGKIEHVALAVADLDAAVAHYEKVWGLEVSHRERVEDQGVEEA
                     MLPLGESYLQLLGPTGPDTTVGKFVAKRGEGLHHIAYEVDDLEAALAELKDKGVPLID
                     EVPRRGGRGHMVAFVHPKGNHGLLVELIQRRRPRPAAQGGRPSH"
     gene            3010..4257
                     /locus_tag="VEV43_10540"
     CDS             3010..4257
                     /locus_tag="VEV43_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP24002.1"
                     /translation="MSQTDAAAVLSPWRRPDFVKLLAISITVALGFGMVVPVLPLFAR
                     SFGVGLGTLGLVNVVFGGTRFAFGIVGGLVVDRFGERACTIAGLLIVSASSYAAGLAG
                     SFPQLVLARGFGGAGSALFINGLMNRILRIIEPEAMGRATGAFRSSFLVGIGAGPVFG
                     GVIADQFGLAAPFHFYATGLLLSAAIAWFVMAGEQGRGEGERKTPIEALRAAGPLFRD
                     YRYTIALLATLVGWWTISGPAQFVGPIFAEDVLGLSEGQIGLAVTMLSVGEILILFVA
                     GRAADKYGRRAVLVPSLAVAAVSTALLGRIEETPLAYYPLMVALGASIAAGGTAAGGL
                     LADSIPRSGSGAAVGVNQMAGDLGYLTSPSAIGAVADAGSYRTAYLVGALPAAIAFVA
                     ALRLPKGRSTGREHPPAEPHPVG"
     gene            complement(4258..5193)
                     /locus_tag="VEV43_10545"
     CDS             complement(4258..5193)
                     /locus_tag="VEV43_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24003.1"
                     /translation="MQFIRDLREVTGALKELVADDDRHEEDAKLELAVSAQRFSQETR
                     EVVDDKLSFSATLMRAGEVDAATRLLAEVEAEVRDNEAALIESVNEVKVARAARRERI
                     TRMRLARSLAVAMLGAAVMATSAAGMAFASFLEDRAQAEAAAERRHDRRRGDGPARAA
                     AAAAARADRTRPAAKVKRVELRGVEAALTAKAFAAFEQLKSGDVEPAELEHLLSLLPA
                     ELAETMRQALATTKVAEKTVKDALPILSRPAAKKKADKADQAADGAEKQDPPAEEPKD
                     EEPDPEPDPEPTQSPGDEEPDDEDGTMLSGITGGD"
     gene            5337..>6587
                     /locus_tag="VEV43_10550"
     CDS             5337..>6587
                     /locus_tag="VEV43_10550"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HYP24004.1"
                     /translation="MSTQEVPQEQATRPIDEQVERLRSLRDEARSGGGGAAVERQHER
                     GKLTARERIEKLLDAGSFVETDMLVRHRSHGFGIEGKRPLSDAVVTGWGTIDGRKVFV
                     FAQDFTVFGGSLGEVMGEKICKIMDLALQTGAPVIGLNDSGGARIQEGAASLAGYGYI
                     FDRNVRSSGVIPQISAIMGPCAGGAVYSPAMTDFTFMVTETSHMFITGPEVIKAVTGE
                     TVTQEELGGAMSHASKSGVAHFVAGSDEDCLAQVRYLLSFIPSNNFESPPYFPPADDP
                     MRSCDDLLDLIPDSPNKPYDMHAVLSSVFDDGEFFEVHQHWAQSILCGFARLAGHPVG
                     IVANQPQVLAGTLDIASSTKAARFVRFCDAFNIPLVTFVDVPGFLPGTEQEYGGIIRH
                     GAKLLYAFSEATVPRMTVITRKAYG"
BASE COUNT          840 a         2350 c         2358 g         1039 t
ORIGIN      
        1 ttgagctggg ggccgctgcc ggtcctcgtc cccctgttcg tcgagcagga gctcggcgag
       61 ggcgcgaccg cgctcggctt cgtcttctcc ggctacggcc tcgggggcct ggcggggacc
      121 gtcttcgccg ggaccaaggg catgcgcttc gacagcgtgg tccccgcgta cctcggatgg
      181 ggcctcggca ccgtcgcgat cggcgcgctg gcgttcgcgc cgagcgccgt cgccgcagcc
      241 gcgctcatgg ccgtcgtcgg cttcaccggc cagatcgccg aggtcacctg ggcgacgctg
      301 ctgcagaagt tcgtgccgcg cagcctgctg ggacgcgtcg tctcgacgga ctggctcgtg
      361 tcgctgagcc tccagcccct gggcgtagcg ctggcggtcc cggtggcggc ggcgataggc
      421 attccggccg cgttcgcggg aggggccgcc ctggtgctcg cggcgatggc gctgggactg
      481 tggtggaggc cggtgcgaga gatcccggag ccggtctagc ccgcgacgcg gaagatgagc
      541 gcgtcgccct ggcccccgcc gccgcacagg gccgccgcac ccaggccccc gccgcggcgc
      601 ttcagctcgt gcagcagcgt caccgtcagg cgcgcgcccg tgcagccgat ggggtggccg
      661 agcgctaccg cgccgccgtt cacgttgacc ttgtcctccg ccgacttgtc gccgttgaag
      721 agcatccgcg tcgagtggat cgccaccgcc gcgaacgcct cgttgatctc gacgaggtcg
      781 aggtcgttcg cggagagccc ggccttcttc agcgcggcct gcaccgcgag cgccggcacc
      841 gtgtggaggt acggcgggcg gtcggccgac atgccgtggg cgacgatctc ggccatcggg
      901 gtgatgccga gggcctcggc gcgctccgcg ctcatcacga cgacggccgc gccgccgtcg
      961 gagatctgcg aggcgttccc cgcggtgatc gtgccgtcgg tgcggaacgc gggacggagc
     1021 ttcgcgagcg tctcggaagt cgtctgcgcg cgcacgccct cgtcggtcgc gaactggacc
     1081 gggtcgccct tgcgctgcgg gatctcgaac gggacgatct cctcgtcgaa gcgtccctcg
     1141 gagatcgcgg cggccgcgcg ctggtgcgac ctcagcgacc attcgtcctg ggcctcgcgg
     1201 gacacctcga actccatgtt cacgtcctcg gactggtcgc ccatgtgctt gtcggtgaac
     1261 gcgtcccaga gaccgtcgaa gatcatcgag tcgaccagct tcccgtcgcc catgcggtaa
     1321 ccgttgcgcg ccttcggcaa caggtagggg gcctggtcca tcgactccat gcccccggca
     1381 acgacgacgt cgacctcgcc cgcgcggatc agctggtcgg cgagcgcgat cgcgttcagc
     1441 cccgacaggc acaccttgtt gatcgtcagc gccggcaccg tgtccgggat ccccgctttg
     1501 atcgcggcct ggcgggcggt gatctgcccc gtccccgcct ggaggacctg gcccatgatc
     1561 acgtagtcga cctggtcgcc tgagctcccg gcgcgctgca gcgcggcctc gatcgcgcgg
     1621 cccccgaggt cgaccgccgg gacgttcgcg agggcgccgc cgaacttgcc gatcgcggtg
     1681 cgtgcggccg atacgactac ggacgatgcc atgaggaagc tcctttgcga cccgggttcg
     1741 tgaagagagc aaccattcta gagtcgcctc tatgcccacg ctgaggacgg aacggcggga
     1801 ccgcacgctc gtggtcacga tcgaccggcc ccaggtccgc aacgcggtcg accgctccac
     1861 cgccgacgct ctctccgccg cgttcggggc gttcgaggcc gacgacgagg ccgacgtcgc
     1921 ggtcctgacc ggagcgggcg gccacttctg cgccggggcc gacctcaagg cgatgggcga
     1981 ggacccggac cgggcgaacc gggtcgagcc cgagggcgac ggcccgatgg gcccgacgag
     2041 gatgctcctg cgcaagcccg tgatcgcggc gatcgagggc tacgcggtcg cgggagggct
     2101 cgagctcgcg ctgtggtgcg acctccgcgt cgccgccgag gacgccgtgc tcggagtctt
     2161 cagccgccgc ttcggagtcc cgctgatcga cgggggaaac gtgcggctcc cgcgggtcgt
     2221 ggggctcggc cgcgcgctcg acatgatcct caccgcccgg gcggtcgacg cacccgaggc
     2281 gctgtcgatc ggtctcgtca accgcgtctg cgcgccgggc gcggccctcg agaccgcgct
     2341 cgacctcgcg cagacgatcg cgtcgtggcc gcagggaccg ttgcgcggcg acaggctcgc
     2401 ggcgatagaa gcttcgtcgc tcccgctcga cgacgcgatg cggaacgagt tccaccgcgg
     2461 cgtcgaatcg ctgagcgccg gccagacgct tccgaacgtc cgccgattcg cggccggaga
     2521 ggggcggcac ggcgagggca cccgctagcc gctcttagac tctgccgatg ctcgggaaga
     2581 tcgaacacgt cgcgctcgcc gtcgccgacc tcgacgccgc cgtcgctcat tacgagaagg
     2641 tctggggtct cgaggtctcg catcgcgagc gcgtcgagga ccagggcgtc gaggaggcca
     2701 tgctcccgct cggcgagagc tacctccagc tcctcggccc caccggcccc gacaccaccg
     2761 tcggcaagtt cgtcgccaag cgcggagagg ggctgcacca catcgcgtac gaggtcgatg
     2821 acctcgaggc cgcgctcgcc gagctcaagg acaagggcgt cccgctgatc gacgaggtcc
     2881 ctcgccgcgg gggccgcggc cacatggtgg cgttcgtgca cccgaagggg aaccacggcc
     2941 tgctcgtaga gctcattcag cgccggcggc ctcggccggc ggcccagggc ggacggccct
     3001 cgcactaacg tgtcgcagac ggacgccgcg gcggtcctct cgccgtggcg gaggcccgac
     3061 ttcgtcaagc tcctggcgat ctcgatcacg gtcgcgctcg gcttcgggat ggtcgtgccc
     3121 gtgctgccgc tgttcgcccg gtcgttcgga gtcgggctcg gcaccctcgg cctcgtcaac
     3181 gtcgtgttcg gcggcacgag gttcgcgttc gggatcgtcg ggggcctcgt cgtcgaccgc
     3241 ttcggcgagc gcgcctgcac gatcgcgggc ttgctgatcg tctcggcctc gtcctacgcg
     3301 gccggcctcg ccggctcgtt cccgcagctc gtcctcgccc gcggcttcgg cggcgccggc
     3361 tcggccctgt tcatcaacgg cctcatgaac cggatcctca ggatcatcga gcccgaggcg
     3421 atgggccgcg cgaccggcgc gttccgctcg tccttcctcg tcggtatcgg cgcgggcccg
     3481 gtcttcggag gcgtgatcgc cgaccagttc ggcctcgcgg cccccttcca cttctacgcg
     3541 accgggctgc tgctgtcggc ggcgatcgcg tggttcgtga tggcgggcga gcagggccgc
     3601 ggcgagggcg agcgcaagac gccgatcgaa gcgctgcggg ccgccggccc cctgttccgc
     3661 gactaccgct acacgatcgc gttgctcgcg accctcgtgg gctggtggac gatctcgggc
     3721 ccggcccagt tcgtcggccc gatattcgcc gaggacgtgc tcggcctctc cgagggccag
     3781 atcggcctcg ccgtgacgat gctctcggtc ggcgaaatcc tgatcctgtt cgtcgccggg
     3841 agggccgccg acaagtacgg gcggcgggcg gtcctcgtgc cctctctcgc ggtcgccgcg
     3901 gtgtcgacgg cgctgctcgg gcgcatcgag gagacgccgc tcgcgtacta cccgctgatg
     3961 gtcgccctcg gcgcgtcgat cgccgcggga gggaccgcgg cgggcgggtt gcttgcggac
     4021 tcgatcccac ggtccggctc gggcgcggcg gtgggcgtca accagatggc gggagacctc
     4081 ggctacctga cctcgccgtc ggctatcgga gcagtggccg acgcagggtc gtaccggacg
     4141 gcctacctag tgggggccct gcccgcagcg attgcgttcg tggcggcgtt gcgactgccg
     4201 aaggggagat cgacgggccg cgagcatccg cccgcggagc cgcatccggt cggctagtca
     4261 gtccccgccg gtaatgccgg agagcatcgt gccgtcctcg tcgtccggct cctcgtcgcc
     4321 cggcgactga gtgggctcgg ggtcgggctc gggatccggc tcctcgtcct tgggctcctc
     4381 ggcgggcggg tcctgcttct cggccccgtc ggcggcctgg tcggccttgt cggccttctt
     4441 cttcgccgcg gggcgggaca ggatcggaag ggcgtccttg acggtcttct cggcgacctt
     4501 cgtggtcgcg agcgcctggc gcatcgtctc ggcgagctcg gccggcagca ggctcagcag
     4561 gtgctcgagc tcggccggct cgacgtcgcc gctcttgagc tgctcgaacg cggcgaacgc
     4621 cttggcggtc agtgcagcct cgacgccccg gagctcgacc ctcttgacct ttgcggccgg
     4681 tcgcgtccgg tccgcccggg cggcagcggc ggcggcagcg cgagccgggc cgtctccgcg
     4741 gcgccggtcg tgcctgcgct cggcagcggc ctcggcctgg gcgcggtcct cgaggaagct
     4801 cgcgaacgcc atgccggcgg ccgaggtggc catgacggcg gcgccgagca tcgcgacggc
     4861 gagcgaccgc gcgagacgca tccgcgtgat gcgctcgcgg cgggcggctc gggcgacctt
     4921 gacctcgttg acggactcga tgagcgcggc ctcgttgtcg cggacctcgg cctcgacctc
     4981 ggcgagaaga cgggtggccg cgtcgacctc accggcacgc atgagcgtgg cggagaagga
     5041 gagcttgtcg tcgacgacct cgcgcgtctc ctgcgagaag cgctgagccg acacggcgag
     5101 ctcgagcttg gcgtcttcct cgtggcggtc gtcgtcggcg acgagctctt tcagcgcgcc
     5161 ggtgacttcg cggaggtctc ggatgaactg catagaggtg gggttctccc cccggcgtca
     5221 ctcccctgcc accccaggat gacaatccgt ccgatttggc ccggatttca ggcgtgcgtc
     5281 cggggtcggc ctggtgtcgc cgggatgtca gtaggcgcgc cgctagactg ccccgcatgt
     5341 cgacccagga ggtaccccag gagcaggcca ccaggccgat agacgagcag gtcgagcgcc
     5401 ttcgctcgct ccgcgacgag gcccggtcgg gtggtggcgg ggccgccgtc gagcgtcagc
     5461 acgagcgagg caagctcacc gcacgcgagc gcatcgagaa gctcctcgac gccggctcgt
     5521 tcgtcgagac cgacatgctc gtgcggcacc ggtcgcacgg cttcggcatc gagggaaagc
     5581 gcccgctgag cgacgcggtc gtgaccggat ggggcacgat cgacggccgc aaggtcttcg
     5641 tgttcgcgca ggacttcacg gtcttcggag gctcgctcgg cgaggtcatg ggcgagaaga
     5701 tctgcaagat catggacctc gcgctccaga cgggggcgcc ggtgatcggg ctcaacgact
     5761 ccggcggcgc ccgcatccag gagggcgcgg cctcgctcgc cggctacggc tacatcttcg
     5821 accgcaacgt caggtcgtcc ggcgtgatcc cgcagatctc ggcgatcatg ggcccctgcg
     5881 cgggcggcgc cgtgtactcg ccggcgatga ccgacttcac gttcatggtc accgagacct
     5941 cgcacatgtt catcacgggc ccggaggtca tcaaggccgt gaccggcgag acggtgacgc
     6001 aggaggagct cggcggtgcg atgagccacg cgtccaagtc gggcgtcgcg cacttcgtgg
     6061 ccgggagcga cgaggactgc ctcgcgcagg tccgctacct gctgtcgttc atcccgtcga
     6121 acaacttcga gtcgccgccg tacttcccgc cggccgacga tccgatgcgg tcgtgcgacg
     6181 acctcctgga cctgatcccg gactcgccga acaagcccta cgacatgcac gcggtgctct
     6241 cgtccgtctt cgacgacggc gagttcttcg aggtccacca gcactgggcc cagagcatcc
     6301 tctgcggctt cgcacgcctg gcgggtcacc cggtcgggat cgtcgcgaac cagccgcagg
     6361 tgctcgccgg gaccctggac atcgcttcgt ccaccaaggc cgcgcgcttc gtgcgcttct
     6421 gcgacgcgtt caacatcccg ctcgtgacgt tcgtcgacgt accgggcttc ctgccgggga
     6481 ccgagcagga gtacggcggg atcatccgcc acggcgcgaa gctcctctac gcgttcagcg
     6541 aggcgacggt cccccgcatg acggtcatca cgcgcaaggc ctacggc
//
LOCUS       DASQDO010000377         5309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1141515, whole genome shotgun sequence.
ACCESSION   DASQDO010000377 DASQDO010000000
VERSION     DASQDO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141515"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VEV43_10555"
     CDS             complement(<1..394)
                     /locus_tag="VEV43_10555"
                     /inference="COORDINATES: protein motif:HMM:NF017709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I9 family protein"
                     /protein_id="HYP24005.1"
                     /translation="MGARKFVRRGAVLLAGALIAGLPIASGSAQESARERYIVVLERG
                     VPAHATAHEHARSENAEVGFVYEHALNGYSAMMSPQAAERIANRPNVEYVEPDIELHA
                     SAQTLPTGVNRIDGELSPTARINGIDERI"
     gene            complement(520..1881)
                     /locus_tag="VEV43_10560"
     CDS             complement(520..1881)
                     /locus_tag="VEV43_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24006.1"
                     /translation="MKTFARSTFAVGASAALLVTAAPPAAAVRITCGGRTPTITGTSG
                     PDTLRGTNGTDVILAKGGADVVRGLGGRDFICGGGGRDRIVAGAGNDLVKGGPGSDTV
                     TGGADRDQLLGNGGSDRLRGASGPDILLGGNGRDRLDAGDGADYLAGDTGNDVMDGGG
                     GLDQVSYRDARGGVRVNLGSGTSGGAAGNDRLAGIEALRGSTFDDSLTGGPNADAISG
                     LFGDDRINGGGGGDVIDPGPGNDRLDGGAGFDFVDYTSVKGGGVDVNLGSSAHAEGTD
                     RLRALEGVLGSEQADEITGSADNDSLFGGGGRDRLDAGAGDDTLEGGAGADVLQGGEG
                     FDFVLYLFSNRGVTVDLAAGSATGEGTDQLGGIEAANGSEHGDTISGDAGANALAGAG
                     GNDTVFGRDGNDELDGGSGNDTLDGGAGTDECTDGETLAGCEESEARRAAKTPVYARS
                     RRP"
     gene            2052..3068
                     /locus_tag="VEV43_10565"
     CDS             2052..3068
                     /locus_tag="VEV43_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24007.1"
                     /translation="MSDWDRQTTPGGVEGPQWVSAAEAADVAGVTPGTVRYWARNGLI
                     VSEVVEGPAGEKTLVRLDEVLQHARQEAPSRPAPPGPRVGPPPAPGAAGGDVVTDLPA
                     RTSELAPILKSIPEIMAQLTSATDRAARAETKVEFLSAQVTELRRRLAEVEAGAATDG
                     TPSAVMPEAWEQSEPLAATIAEETPDPAPEVTGWPAPPADALQDVPAAEASPDLRSVP
                     PAEAAEPGPEIVDWTTRDPSAGPEDAPAAEEVAVDETPALSAPADDAGDLSLDDIWNQ
                     PAAPGPAPLDEAPEPAPSEVAWHEPAGRAPAPPPPPRAANAPVPEGDDFYGPRRRRWF
                     KRRR"
     gene            complement(3082..3291)
                     /locus_tag="VEV43_10570"
     CDS             complement(3082..3291)
                     /locus_tag="VEV43_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24008.1"
                     /translation="MVTMAQVQPLEEFAQDPLLTVASVLLIVVSIGWIVVSRLVSRNL
                     RKAARARNEKKGAVKPARDIWAGPP"
     gene            3332..4156
                     /gene="larE"
                     /locus_tag="VEV43_10575"
     CDS             3332..4156
                     /gene="larE"
                     /locus_tag="VEV43_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HYP24009.1"
                     /translation="MSALAAKNDALEHALRGYGSVVVAFSGGVDSAFLTAVAHDVLRD
                     EVLAVTAVSPSLARREREGAAALARRYGWHHRMVETHEVERPEYTRNAADRCYWCKDE
                     LFEVLAPIAADRGAAIAVGTNTDDLGDHRPGLRAARERDVRSPLVDAGMSKADVRALA
                     AKRGLPVADKPASPCLASRVAYGVEVSPERLRRIDAAEEAVRALGFDVLRVRDHGDLA
                     RVEVPVEDFARAASLRDEMHARLVELGFRFVTLDLAGFRSGSLNAVLAPPALRRGE"
     gene            4185..5159
                     /locus_tag="VEV43_10580"
     CDS             4185..5159
                     /locus_tag="VEV43_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP24010.1"
                     /translation="MTTDTPVLAEGLVRSFGDVEAVRGIDLALSSGEIFGFLGPNGAG
                     KSTTVRMLTTLLKPSAGRARVAGYDVVAEAHQVRRSIGVALQDAAIDPLMTGRELLKL
                     QAVLHGIDKREGRRRSDELLDTVGLTAAADRRVGTYSGGMRRRLDLALALVHQPVVLF
                     LDEPTTGLDPNSRVALWDEVRRLNRDAGTTVFLTTQYMEEADQLAGRIAIIDNGHIVA
                     DGTPSVMKAKVGDPTLRVAIGPEEDRIAVEAALLEFGEPAPGSADYVAIRLKGGASRI
                     AAVARTLEDAGIAFAGLELLSPSLDDVFQQATGRRLEGAAEPEAAPAR"
BASE COUNT          700 a         2008 c         1864 g          737 t
ORIGIN      
        1 cgatgcgctc gtcgatcccg ttgatccggg ccgtcgggct gagctcgccg tcgatccggt
       61 tgacgcccgt gggcagggtc tgcgccgacg cgtgcagctc gatgtccggc tcgacgtact
      121 cgacgttcgg acggttcgcg atccgctcgg cagcctgcgg cgacatcatc gcggagtagc
      181 cgttcagagc gtgctcgtat acgaacccga cctcggcgtt ctcggaccga gcatgctcgt
      241 gcgcggtcgc gtgcgccggc acgccccgct cgagcacgac gatgtacctc tctcgagccg
      301 actcctgggc cgagcccgac gcgatcggca gaccggcgat cagagcgccc gcgagcagaa
      361 ccgcgccgcg gcgaacgaac ttccgtgctc ccatgcatcc cccctggatg acacgcgagg
      421 gatcgccgat gaccccccga ctggtgggcc agttttcgag ggaggaatgt ccgattcctc
      481 cctttcacag ggagattgtt aagaaaaaat taaagtgttt taaggccttc tcgaccgggc
      541 atagacgggg gttttcgccg ccctgcgggc ctcgctctcc tcgcaccccg ccagggtctc
      601 gccgtccgtg cactcgtcgg tgccggcgcc gccgtcgagg gtgtcgttgc ccgacccacc
      661 gtcgagctcg tcgttgccgt cccggccgaa caccgtgtcg ttgcccccgg cccccgccag
      721 cgcgttcgcg ccggcgtcgc ccgagatcgt gtcgccgtgc tccgagccgt tcgccgcctc
      781 gatgccgccg agctggtcgg tcccctcacc ggttgcggac cccgccgcga ggtcgaccgt
      841 gacgccgcgg ttcgagaaga gatagagcac gaagtcgaaa ccttctccgc cttggaggac
      901 gtcggcgccg gcaccaccct cgagcgtgtc gtcaccggcc cccgcgtcga gccggtcacg
      961 accgccgccg ccgaacaggg aatcgttgtc ggcggagccg gtgatctcgt ctgcctgctc
     1021 cgacccgagg acgccttcga gggcccggag gcggtcggtc ccctccgcat gggccgagga
     1081 accgaggttg acgtcgacgc ctcccccctt caccgaggtg tagtcgacga agtcgaagcc
     1141 ggcgccaccg tcgagtcggt cgttgccggg cccgggatcg atcacgtcgc cgcccccgcc
     1201 cccgttgatc cggtcgtcgc cgaagagccc ggagatcgcg tcggcattcg ggccgccggt
     1261 caggctgtcg tcgaaggtcg accctctcag cgcctcgatt cctgccaagc ggtcgttgcc
     1321 ggcggcaccg cccgacgtcc cgcttccgag gttcacccgc acgcctccgc gcgcgtcgcg
     1381 atacgagacc tggtcgaggc ccccgccccc gtccatcacg tcgttgccgg tgtcgcccgc
     1441 caggtagtcg gcaccatccc cggcatccag gcggtcgcgc ccgttgcccc ccaggaggat
     1501 gtcgggacca ctcgcgccgc gcagccggtc gctgcctccg ttgccgagga gctggtcccg
     1561 gtcggccccg ccggtgaccg tgtccgagcc cggaccgccc ttcacgaggt cgttcccggc
     1621 ccccgcgacg atcctgtccc ggccgccgcc gccacaaatg aagtcgcggc cgccgagccc
     1681 gcgcaccacg tctgctccgc ccttcgcgag gatcacgtcc gtcccgttcg tcccgcgcaa
     1741 ggtgtccggg ccggacgtcc ccgtgatcgt cggggtccgg ccgccgcaag ttatgcggac
     1801 ggccgccgcc ggcggtgccg ccgtcacgag aagggcggcg ctcgcgccca cggcgaaggt
     1861 ggagcgggcg aatgtcttca tggggctctc cttggatgga tggactcgtc cggtgcgcgc
     1921 ggttgctccc gaaaagctaa accgcgccgg ggctgcacgg tcaaccgggg tcccgctagg
     1981 ctctgggctg cttgtccggc ctcaggggcc ggtgaccacc acccgaggcc gactctgaag
     2041 ttgggggttc gatgagcgat tgggaccgtc agacgacgcc gggcggcgtc gagggcccgc
     2101 aatgggtctc ggcagccgag gcggccgacg tcgcgggcgt gaccccgggg accgtgcggt
     2161 actgggcgcg caacggcctg atcgtctccg aggtcgtcga ggggccggcg ggcgagaaga
     2221 cgctcgtgag gctcgacgag gtcctccagc acgcccgtca ggaggcaccc tctcgacccg
     2281 cgccgccggg cccccgggtg ggccctccgc cggctccggg cgctgcgggc ggggacgtcg
     2341 tcaccgacct gccggcacgg acgagcgagc tcgccccgat cctcaagtcg atccccgaga
     2401 tcatggccca gctcacgtcc gcgaccgacc gggccgcacg ggccgagacg aaggtcgagt
     2461 tcctctcagc gcaggtcacg gagctgcgcc gccgcctcgc cgaggtcgaa gccggagcgg
     2521 cgaccgacgg gaccccgtcc gcggtgatgc cggaagcatg ggagcagtcg gagcccctcg
     2581 cggcgacgat cgccgaggag accccggacc cggcgccgga ggtgaccggg tggcccgccc
     2641 ctcccgccga cgcgttgcag gacgtcccgg ccgcggaagc gtcgccggat ctccggagcg
     2701 tccctccggc cgaggcggcc gagcccggcc ccgagatcgt cgactggacc accagggacc
     2761 cgtccgccgg gccggaggac gcccccgccg ccgaggaggt cgcggtcgac gagacgccgg
     2821 ccctctcggc ccccgctgac gacgccgggg acctctcgct cgacgacatc tggaaccagc
     2881 ccgcggcccc cggacccgcg ccgctggacg aggcgcccga gccggcgccg tccgaggtcg
     2941 cctggcacga gcccgccggc cgcgccccgg caccaccgcc accgccccgc gccgcgaacg
     3001 cgcccgtgcc ggagggcgac gacttctacg gcccccgccg tcgccggtgg ttcaagcgcc
     3061 gccgctagcc gcggcgcccg cttaaggggg acccgcccag atgtcccggg ccggcttcac
     3121 ggcccccttc ttctcgttgc gagcccgagc cgccttgcgg aggttccggc tgacgagccg
     3181 ggacacgacg atccacccga tcgagacgac gatcagcagc acggacgcga ccgtgagcag
     3241 cgggtcctgc gcgaactcct cgagcggctg gacctgcgcc atcgtgacca tcaggccgac
     3301 aagctaaccc cctgctgaga ggatgggacg gatgtcagcc ctggccgcga agaacgacgc
     3361 cctcgagcac gcgctccggg ggtacggctc ggtcgtcgtc gcgttctccg gcggcgtcga
     3421 ctccgctttc ctgacggccg tcgcccacga cgtgctgcgc gacgaggtgc tcgccgtcac
     3481 cgccgtgtcg ccgtcgctcg cgcggcgcga gcgcgagggg gccgcggccc tcgcccgccg
     3541 ctacggctgg caccaccgga tggtcgagac gcacgaggtc gagcgaccgg agtacacgcg
     3601 caacgcggcc gaccgctgct actggtgcaa ggacgagctc ttcgaggtgc tcgcgccgat
     3661 cgccgcggac aggggggccg cgatcgcggt cggcaccaac accgacgacc tcggcgacca
     3721 ccggccggga ctgcgcgccg cccgagaacg ggacgtgcgg tctccgctgg tcgacgccgg
     3781 gatgagcaaa gccgacgtgc gcgcgctagc ggcgaagcgc ggccttcccg tcgccgacaa
     3841 gcccgcgtcg ccgtgcctcg cgtcccgcgt cgcctacggc gtcgaggtct cgcccgagcg
     3901 gctccggcgg atcgacgcgg ccgaggaagc cgtgcgcgcc ctcggcttcg acgtgctgcg
     3961 cgtgcgcgac cacggcgacc tcgcgcgcgt cgaggtcccg gtcgaggact tcgcccgggc
     4021 ggcctcgttg cgggacgaga tgcacgcccg gctcgtcgag ctcggcttcc gcttcgtcac
     4081 gctcgacctc gcggggttcc ggtcggggtc cctgaacgcg gtcctggcgc cgccggcgtt
     4141 gcgtcgcggg gaatagcggc tcgcccgctc tggttgcccg ccccgtgacc accgacaccc
     4201 ccgtcctcgc cgagggcctc gtccgcagct tcggcgacgt cgaggccgtc cggggcatcg
     4261 acctcgcgct ctcctcgggc gagatcttcg gattcctggg gcctaacggg gccggcaagt
     4321 ccacgacggt ccggatgctc acgacgttgc tcaagccttc cgcgggccgc gcccgcgtcg
     4381 cgggctacga cgtcgtcgca gaggcgcacc aggtgcgccg ctcgatcggc gtcgcgttgc
     4441 aggacgccgc gatcgacccg ctcatgacgg gacgcgagct gctgaagctc caggccgtcc
     4501 tgcacggcat cgacaagcgt gagggacggc ggcggtccga cgagctgctc gacaccgtcg
     4561 gtctcaccgc cgcggccgac cgcagggtcg ggacgtactc gggcggcatg cggcggcggc
     4621 tcgacctcgc gctcgcgctc gtgcaccagc ccgtcgtcct gttcctcgac gagccgacga
     4681 cggggctcga cccgaactcg cgcgtcgcac tgtgggacga ggtccggagg ttgaaccgcg
     4741 acgccgggac gacggtgttc ctcacgacgc agtacatgga ggaggccgac cagctcgcgg
     4801 gccgcatcgc gatcatcgac aacggccaca tcgtcgcgga cggcacgcct tcggtgatga
     4861 aggcgaaggt cggcgacccc acgctgcgcg tcgcgatcgg ccccgaggag gaccggatcg
     4921 cggtcgaggc cgcgctgctc gagttcggcg agcccgctcc gggatccgcc gactacgtcg
     4981 cgatcaggct gaagggcggg gcgagccgca tcgccgcggt ggctcggacg ctcgaggacg
     5041 cgggcatcgc gttcgccggc ctagagctcc tctcgccgtc gctcgacgac gtcttccagc
     5101 aggccacggg gcggaggctc gagggggccg cggagcccga ggctgcgccg gcgcggtgag
     5161 cgcgctcgac cagtacgtcg cgctcagccg gcgtgcgatc gtcaccacgt tccgccagcc
     5221 gacggcgatc atcccggcga tgacgttccc gctgttgttc atggcgctgt cgtcggcggc
     5281 gttcgagcgg tcgacgagcc tccccggct
//
LOCUS       DASQDO010000378         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_468219, whole genome shotgun sequence.
ACCESSION   DASQDO010000378 DASQDO010000000
VERSION     DASQDO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468219"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VEV43_10585"
     CDS             complement(<1..266)
                     /locus_tag="VEV43_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HYP24011.1"
                     /translation="MAELLDLAQSIAGRAGDGELVEAYVTHERDVYAKAFEGEIEATS
                     AAEPRGAGVRLISGGRVGFAYTTDLTHGGLSDVVAQARENSASAT"
     gene            complement(266..1648)
                     /locus_tag="VEV43_10590"
     CDS             complement(266..1648)
                     /locus_tag="VEV43_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HYP24012.1"
                     /translation="MLAEETIKKVLAAALTGGGDLAELFVEDRSSTGLRLEDRRVEDV
                     ASGRDAGAGVRVVAGDRSSYAYTNLLTEEGLVETANAARAGLAGTAGAVADLTRTQPP
                     VEHPVRTPPAEVAAADKVEALRAADDAARAAGGEVRQVVASYQDVRQKVLIAASDGLL
                     VEDDRTRVRFVCQVVAARDGQISTGFRAPGHSGGFELLEAFPAAEVGALAAEKAITML
                     DARPSPAGEFPVVLAPGAGGVLIHEACGHGLEADTLVKNASVYANRAGERFGSDDVTI
                     VDDGGDVGAWGSIGVDDEGTPAQRTVLFDRGILTGHLSDRLSAEKIGHPPTGNGRRQS
                     YAHLPIVRMTNTYLLPGPDDPEEIVRSVQRGVYAASFAGGEVNPATGNFVFGMSEAYM
                     NEDGQLTYPIKGAQLIGNGPKVLNVIEAIAADFDRWEGVCGKDGQHAPVTNGMPTVLL
                     GTMTVGGTEA"
     gene            1732..2100
                     /locus_tag="VEV43_10595"
     CDS             1732..2100
                     /locus_tag="VEV43_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24013.1"
                     /translation="MRRAPSGLSRSGRSLVLGVQPQGYTAEQFERLRQIHDKLTAARR
                     ELDALIGDAYLRPRASIVPVHFLDLASAVEKLRWAEKEWRELDRELAEAAPAQASDSS
                     EADDGEPHHAEREPVTAETR"
     gene            complement(2177..>2276)
                     /locus_tag="VEV43_10600"
     CDS             complement(2177..>2276)
                     /locus_tag="VEV43_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HYP24014.1"
                     /translation="AEYAALVRHVVENPMINGEVIRLDAALRMPPR"
BASE COUNT          305 a          830 c          801 g          340 t
ORIGIN      
        1 gtcgcgctgg cggagttctc gcgcgcctgc gcgacgacgt cgctcaaccc cccgtgggtg
       61 aggtcggtcg tgtaggcgaa cccgacgcgg cctccggaga tcagccggac cccggctccg
      121 cgcggctcgg ccgcggacgt cgcctcgatc tctccctcga aggccttcgc gtagacgtcc
      181 cgctcgtgcg tgacgtacgc ctcgacgagc tcaccgtcgc cggcgcgacc ggcgatcgac
      241 tgcgcgaggt cgaggagctc ggccactagg cctccgtccc cccgacggtc atcgtgccga
      301 gcaggaccgt cggcatcccg ttcgtgaccg gcgcgtgctg gccgtccttg ccgcagacgc
      361 cctcccagcg gtcgaagtcc gcggcgatgg cctcgatgac gttgaggacc ttggggccgt
      421 tcccgatgag ctgcgcgccc ttgatcgggt acgtgagctg cccgtcctcg ttcatgtacg
      481 cctctgacat gccgaagacg aagttgccgg tcgccgggtt cacctcgccg ccggcgaagc
      541 tcgccgcgta gacgccgcgc tgcactgagc ggacgatctc ctcggggtcg tcgggaccgg
      601 gcagcagata cgtgttcgtc atgcggacga tcggcaggtg ggcgtaggac tggcgccggc
      661 cgttgcccgt cggagggtgg ccgatcttct cggcggagag ccggtcgctg aggtgcccgg
      721 tcaggatgcc ccggtcgaac agcaccgtgc gctgcgcggg cgtgccctcg tcgtcgaccc
      781 cgatcgagcc ccaggcgccg acgtcgcccc catcgtcgac gatcgtgacg tcgtcggagc
      841 cgaagcgctc gccggcccgg ttcgcgtaga cgctcgcgtt cttgacgagc gtgtcggcct
      901 cgagcccgtg cccgcacgcc tcgtggatca gcacgccgcc ggcccccggc gcgagcacga
      961 cggggaactc cccggcgggc gagggccggg cgtcgagcat cgtgatcgcc ttctccgcgg
     1021 cgagcgcgcc gacctcggcg gccgggaacg cttcgagaag ctcgaacccg cccgagtggc
     1081 cgggggccct gaaccccgtc gagatctgac cgtcgcgcgc cgcgacgacc tggcacacga
     1141 agcggacgcg cgtccggtcg tcctcgacga ggagcccgtc cgacgcggcg atcagcacct
     1201 tctggcggac gtcctggtaa gaggcgacga cctggcggac ctcgcccccg gccgcgcgcg
     1261 ccgcgtcgtc ggccgcccgg agggcctcga ccttgtccgc tgccgctacc tcggcgggag
     1321 gcgtcctcac ggggtgctcc accggaggct gcgtgcgggt caggtcggcc actgcgccgg
     1381 cggtccccgc gagcccggcc cgggccgcgt tcgcggtctc gaccaggccc tcttccgtca
     1441 ggaggttcgt gtacgcatac gaggagcggt cgccggcgac caccctgacc ccggcccccg
     1501 cgtcgcgtcc cgaggcgacg tcctcgaccc ggcggtcctc gagccggagc cccgtcgagc
     1561 tgcgatcctc gacgaacagc tcggcgaggt cacccccgcc cgtgagggcg gcggccagaa
     1621 ccttcttgat ggtctcctcg gctagcatcc ggccgcagga tacgcgttga gggggcgcgg
     1681 agctgggctt cgtgcgcttg tgcccggaat ctgggccgat gtactctggc ggtgcgccgc
     1741 gcgccctcgg gtctgtctcg ttcaggaagg agcctggttc tgggagtaca gccgcagggc
     1801 tacaccgccg agcagttcga acggctccgg cagatccacg acaagttgac ggcggcaagg
     1861 agggagctcg acgctctcat aggcgatgcc tacctccgcc cccgggcctc gatcgtgccc
     1921 gtccacttcc tggacctggc atcagccgtc gagaagctcc gctgggccga gaaggaatgg
     1981 cgcgagctgg accgcgagct ggccgaggcc gccccggccc aggcatcgga ctcgtccgag
     2041 gccgacgacg gggagccgca ccatgccgag cgcgagccgg tgacggcgga gacgcgctag
     2101 gccccaaggc cgggctacct cttcccagga cccgtctgac atgtctgaca tgtcagacac
     2161 gctttccgaa agagggctaa cgggggggca tgcggagcgc cgcgtccagg cggatcacct
     2221 ctccgttgat catcgggttc tccactacgt gtcgcacgag cgccgcatat tcggcc
//
LOCUS       DASQDO010000379         5396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_713098, whole genome shotgun sequence.
ACCESSION   DASQDO010000379 DASQDO010000000
VERSION     DASQDO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713098"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1237)
                     /locus_tag="VEV43_10605"
     CDS             complement(74..1237)
                     /locus_tag="VEV43_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP24015.1"
                     /translation="MDFSIPAEYDELRRNLTEFVERELKPLEAAELDPESDDMPIELI
                     DRVRRRAAELGFYAADFPEDVGGSGLPQLGMVLLREAAPLTGCRLAGFTTYGPEGPTG
                     LLLAGTDEQKKKYLVPLVTAEKSMCFALTEPDAGSDAQSLRTTATKDGSDWVLNGTKH
                     FITNGKHADFALVFAANDRELKAQGGITAFLVDRGTPGFSVGRAQRGMLDVEGQYELV
                     FEDCRVPEEQVLGGPGNAGMGFYSAMQFLAMGRLSIAATCNGIAEYALGLGIDYAKQR
                     VAFDRPIGKNQYVQGHIVDSLVELKASKLMTYECAWKYDNGETVIQESSIVKLYAAEM
                     VNRVVDRMIQVHGGMGWMRELPLERLYRLVRIFPIVEGTSEIQKYVIAKTLGL"
     gene            1285..1416
                     /locus_tag="VEV43_10610"
     CDS             1285..1416
                     /locus_tag="VEV43_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24016.1"
                     /translation="MTRIRALALIPALLAAFTPAAEGAPRKCFGEKATITGRRLIRN"
     gene            1501..2520
                     /locus_tag="VEV43_10615"
     CDS             1501..2520
                     /locus_tag="VEV43_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24017.1"
                     /translation="MKTTVTLVAAALCAALLPATAQAAPVKCFGEPATIVGTNEDDQI
                     EGTGGRDVIVGLDGDDTINGLGGDDILCGSPGMDQLFGGYGDDRMNGNLGIDRVSGGI
                     EELEEEKASAPDDGDDLARGGPGRDNFIIGHTDGAAFVDLGKGIGKAPGTGRDRIQGI
                     EDTSGTDKGDKLFGSDAANIIYGWDGKDLITGRGGDDSLVGNYDGGTGDDDTIYGGSG
                     DDGIYGDTGEYHGGSDLLYGGPGADRIYGEILAYVDYAYPDEIHGGPGPDEIWGDYAS
                     DDEGDIFEDVGEPDVITGGGGNDTMHGMAGNDSLDGGAGNDFAEGGEDYDRCVDSEEV
                     VDCEE"
     gene            complement(2546..3202)
                     /locus_tag="VEV43_10620"
     CDS             complement(2546..3202)
                     /locus_tag="VEV43_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYP24018.1"
                     /translation="MAFPKNLLAEHEKLIFDLKPHWVALVPSILWTLVAIAALFLGYR
                     GVDAAFSDNEDTAKMVVGAVVTIGWYLLALLPAMRWRWTMFVLTSDRLITRSGIVAKH
                     SREIPLERINDVTFTQTVLERALGAGDLLVESAGERGQTRITDVRKPEQVQLMIYKQT
                     EDNSTRIVRGQMGEVAGHTAPVEPQVTEQLEQLGRLRKDGVLTEEEFQAKKAELLKRL
                     "
     gene            3311..3604
                     /gene="gatC"
                     /locus_tag="VEV43_10625"
     CDS             3311..3604
                     /gene="gatC"
                     /locus_tag="VEV43_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYP24019.1"
                     /translation="MPIDRAAVDHVARLARLDLTDDERERMRAELSQILEHAEKIQAL
                     DLDGVPPTSHSVSIRNALRPDEVTPSLAPDEVLANAPEVEDGRIKVPRIIEDA"
     gene            3605..5083
                     /gene="gatA"
                     /locus_tag="VEV43_10630"
     CDS             3605..5083
                     /gene="gatA"
                     /locus_tag="VEV43_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HYP24020.1"
                     /translation="MAELVWTSAVELADALASGDVSAVEVTQAFVDRSAAVEGSISAY
                     LEVTSERALADAKAADERRAAGEARSRYDGIPIAYKDLFVTKGVRSTSGSKILDNFVP
                     PYDATVVARCSDAGLPMLGKLNMDEFAMGSSTENSGYGPSRNPWDTNRVPGGSSGGST
                     ALVAAGGAPWTWGTDTGGSVRQPAALCGLVGVKPTYGLVSRYGMIAFASSLDQAGPIT
                     RTVKDSAALLGLAAGGDRMDSTCIPGGRADFLAGIEDGIDGLRIGIMPELESGEGTQP
                     GVLTRVQEAYQRLEKLGATLEEVSLPSFEYGLSAYYLLAPAEASSNLARYDGVRYGHR
                     AAGAEDIVRMTSRTREEGFGAEVKRRIMLGTYALSAGYYDAYYGKAQKVRTLITRDLE
                     RAYETVDLIACPTSPTTAFEIGSRTADPLAMYLSDVFTVPVNLAGNAAISVPCGTSPD
                     DGLPVGLQLIAKSLDEPTMFRAAHAFERDLGWIDSPEGRPSL"
     gene            5080..>5396
                     /locus_tag="VEV43_10635"
     CDS             5080..>5396
                     /locus_tag="VEV43_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HYP24021.1"
                     /translation="MSVTETDVYETTVGLEIHVELSTNSKMFCGCAVAFGGDPNTRTC
                     PVCLGLPGSLPVPNERAIDYTMRIALALNCRIAETSLFHRKNYFYPDMPKNYQISQYD
                     QPLG"
BASE COUNT          883 a         1867 c         1838 g          808 t
ORIGIN      
        1 cggagccagg gttcggcgac gagatctgcc ggtccgaaga aggagagccc ggctacgcgt
       61 taccgccgag gagctagagc cccagcgtct tggcgatcac gtacttctgg atctccgacg
      121 tcccctcgac gatggggaag atcctcacga gcctgtagag acgctcgagc gggagctcgc
      181 gcatccagcc catgcccccg tgcacctgga tcatcctgtc gacgacacgg ttgaccatct
      241 cggcggcgta gagcttcacg atcgacgact cctggatcac cgtctcgccg ttgtcgtact
      301 tccacgcgca ctcgtacgtc atcagcttcg acgccttgag ctcgaccaac gagtcgacga
      361 tgtggccctg gacgtactgg ttcttgccga tgggccggtc gaacgcgacg cgctgcttcg
      421 cgtagtcgat cccgagcccc agcgcgtact ccgcgatccc gttgcacgtc gccgcgatcg
      481 agagccgtcc catcgcgagg aactgcatcg ccgagtagaa gcccatgccg gcgttgcccg
      541 gccccccgag gacctgctcc tccggcaccc tgcagtcctc gaacacgagc tcgtactggc
      601 cctcgacgtc gagcatcccg cgctgcgcgc gcccgacgga gaagccgggc gtgcccctgt
      661 cgacgaggaa cgcggttatc ccgccctggg ccttcagctc gcggtcgttc gccgcgaaca
      721 cgagcgcgaa gtccgcgtgc ttgccgttcg tgatgaagtg cttcgtcccg ttgaggaccc
      781 agtccgaccc gtccttggtc gccgtcgtcc gcaggctctg cgcgtcggac cccgcgtccg
      841 gctcggtgag cgcgaagcac atcgacttct ccgcggtcac gagcggcacg aggtacttct
      901 tcttctgctc gtcggtcccg gcgagcaaca ggccggtcgg gccctcgggg ccgtaggtcg
      961 tgaaccccgc gaggcggcac cccgtcaacg gcgcggcttc gcgcagcagc accatcccga
     1021 gctgaggcaa gccgctgcct ccgacgtctt ccgggaagtc ggccgcgtag aacccgagct
     1081 cggccgcccg gcgacggacc ctgtcgatca gctcgatcgg catgtcgtcg gactccgggt
     1141 cgagctcggc ggcttccaga ggcttcagct cgcgctcgac gaactcggtg aggttgcgcc
     1201 gcagctcgtc gtactccgcg gggatcgaga agtccatgcc gccacgatac ggaactaacg
     1261 ggggtgcccg aggatcgtct tcgtatgacg agaatccgtg ccctggctct catcccggcc
     1321 ctgctcgccg cgttcacgcc cgcggccgag ggtgcgccgc gcaagtgctt cggcgagaag
     1381 gcgacgatca ccggccgtcg actcatccgg aactagcgcc cgaacgctca gaagtgagcg
     1441 gacgcggccc ccgttccagt cgatgcttgg cctatcccct atggaaggac cagcatcgtc
     1501 atgaagacca cagtgacact cgttgccgcg gcgctctgcg cggcactcct tcctgccacg
     1561 gcccaggccg cccccgtcaa gtgcttcggc gaacccgcga cgatcgtggg gacgaacgag
     1621 gacgaccaga tcgaaggcac tggaggccgg gacgtgatcg tcggcctcga cggcgacgac
     1681 acgatcaacg ggctcggcgg agacgacatc ctctgcggca gcccgggcat ggaccagctg
     1741 ttcgggggtt acggcgacga ccgcatgaac gggaacctcg gcatcgacag ggtctcgggc
     1801 ggcatcgagg agctcgagga agagaaggct tctgcgccgg acgacggcga cgaccttgcc
     1861 cggggcggcc cgggccgcga caacttcatc atcggccata cggacggcgc ggccttcgtg
     1921 gacctcggga aagggatcgg caaggcgcct gggaccgggc gagaccgcat ccaggggatc
     1981 gaggacacgt cggggaccga caagggcgac aagctgttcg gctcggacgc cgccaacatc
     2041 atctacggct gggacggcaa ggatttgatc acgggccggg gcggagacga cagcctcgtc
     2101 gggaactacg acgggggaac cggggacgac gacaccatct acggcggtag cggggacgac
     2161 gggatctacg gggacaccgg ggagtaccac ggcgggagcg acctcctcta cgggggcccg
     2221 ggtgccgaca ggatctacgg cgagatcctg gcctacgtcg actacgcgta ccccgacgag
     2281 atccacgggg gccccggccc ggacgagatc tggggtgact acgcctccga cgacgaaggc
     2341 gacatcttcg aagacgtcgg cgagcccgac gtcatcaccg gcggcggcgg gaacgacacg
     2401 atgcacggca tggcgggcaa cgactccttg gacgggggcg ccgggaacga cttcgccgaa
     2461 gggggcgagg actacgaccg gtgcgtcgac tccgaggagg tcgtggactg cgaggagtga
     2521 ctctccacgg ttgcgggccg ggctgctaga ggcgctttag cagctcggcc ttcttcgctt
     2581 ggaactcctc ttccgtcagg accccgtcct tgcgcaggcg gcccagttgc tctagctgct
     2641 ccgtgacctg gggctcgacc ggggcggtgt gcccggcgac ctcgcccatc tgcccccgga
     2701 cgatccgcgt cgagttgtct tccgtctgct tgtagatcat cagctggacc tgctcgggct
     2761 tgcgcacgtc ggtgatcctc gtctgaccgc gttcgccggc cgactcgacg agcaggtcgc
     2821 ccgcgccgag cgcgcgctcg agcaccgtct gcgtgaaggt gacgtcgttg atgcgctcga
     2881 gcgggatctc gcgtgagtgc ttggcgacga tcccgctgcg cgtgatcagc cggtcgctcg
     2941 tgagcacgaa catcgtccag cgccagcgca tcgcaggcag cagcgcgagg aggtaccagc
     3001 cgatcgtgac gaccgcgccg accaccatct tcgccgtgtc ctcgttgtcg ctgaaggccg
     3061 cgtcgacgcc gcggtagccg aggaagaggg cagcgatcgc gacgagcgtc cagaggatcg
     3121 acgggacgag cgccacccag tggggcttga ggtcgaagat gagcttctcg tgctcggcga
     3181 ggaggttctt gggaaaggcc atggacgaac cgtagcaacc gggacgggcc aaggaaagga
     3241 gccccgccga ggcggcccgc gcgtttccct cgggcgtccc gccgaggcgg cccgcgcgta
     3301 ggatgccccg atgcccatcg acagagcggc cgtcgaccac gtcgcgcgcc tcgcgcggct
     3361 cgacctgacc gacgacgagc gcgagcggat gagggccgag ctctcccaga tcctcgagca
     3421 cgccgagaag atccaggccc tcgacctcga cggcgtgccg cccacctcgc actccgtctc
     3481 gatccgcaac gcgctgagac ccgacgaggt gacgccgtcg ctggcccccg acgaggtcct
     3541 cgcgaacgcg cccgaggtcg aggacggccg catcaaggtc ccgcgcatca tcgaggacgc
     3601 gtaggtggcc gagctggtct ggacctccgc agtagagctc gcggacgcgc tcgcgtccgg
     3661 cgacgtctcc gccgtcgagg tgacgcaggc gttcgtcgac cggagcgcgg ccgtcgaagg
     3721 atcgatctcg gcgtatctcg aggtcacgtc cgagcgcgcc cttgcggatg cgaaggccgc
     3781 cgacgagcgg cgcgccgccg gcgaggcgag gtcccgctac gacgggatcc cgatcgccta
     3841 caaggacctc ttcgtcacga agggcgtgcg gagcacgtcc ggctcgaaga tcctcgacaa
     3901 cttcgtcccg ccctacgacg cgaccgtggt cgcccgctgc tcggacgcgg ggctcccgat
     3961 gctcggcaag ctcaacatgg acgagttcgc gatggggtcc tcgaccgaga actcgggcta
     4021 cggaccgtcg cgcaacccct gggacacgaa ccgcgtgccc ggaggatcga gcgggggctc
     4081 gaccgcgctc gtcgcggccg gcggtgcccc gtggacgtgg gggaccgaca cgggtggatc
     4141 cgtccgccag cccgccgcgc tgtgcggcct cgtcggcgtc aagcccacct acggcctcgt
     4201 gtcgcgctac gggatgatcg ccttcgcgtc gtcgctcgac caggccgggc cgatcacgag
     4261 gaccgtgaag gactcggcgg cgttgctggg gctcgccgcc ggcggcgacc ggatggattc
     4321 gacgtgcatt cccggtggcc gtgcggactt cctcgcgggg atcgaagacg ggatcgacgg
     4381 cctccgcatc gggatcatgc cggagctcga gtccggagag ggcacgcagc ccggagtcct
     4441 gacgcgggtc caggaggcat accagcggct cgagaagctc ggtgcgacgc tcgaagaggt
     4501 ctcgctgccg tcgttcgagt acggcctctc cgcctactac ctgctcgcac cggccgaggc
     4561 gtcgtcgaac ctcgcccggt acgacggcgt ccgctacggc caccgcgccg cgggggccga
     4621 ggacatcgtg aggatgacgt cgcggacgcg tgaggagggc ttcggcgccg aggtgaagcg
     4681 ccgcatcatg ctcgggacct acgcgctgtc cgccggctac tacgacgcct actacggcaa
     4741 ggcgcagaag gtcaggacgc tgatcacgcg cgacctcgag cgcgcctacg agaccgtcga
     4801 cctcattgcg tgcccgacgt cgccgacgac cgcgttcgag atcggctcgc gcaccgcaga
     4861 cccgctggcg atgtacctgt ccgacgtctt cacggttccg gtgaacctcg ccgggaacgc
     4921 cgcgatcagc gtgccgtgcg ggacgtcccc ggacgacggt ctgccggtgg ggctccagct
     4981 gatcgcgaag tcgctcgacg agcccacgat gttccgcgcc gcgcacgcgt tcgagcgaga
     5041 tctcggctgg atcgactctc cggaaggaag gccctcgctg tgagcgtcac cgagaccgac
     5101 gtctacgaga cgaccgtagg gctcgagatc cacgtcgagc tctcgaccaa ctcgaagatg
     5161 ttctgcggct gcgcggtcgc gttcggcggc gacccgaaca cacgcacctg cccggtctgc
     5221 ctcgggctgc ccggctccct tcccgtgccg aacgagcggg cgatcgacta cacgatgcgg
     5281 atcgcgctcg cgctcaattg ccgcatcgcc gagacctcgc tgttccaccg gaagaactac
     5341 ttctatccgg acatgccgaa gaactaccag atctcccagt acgaccagcc gctggg
//
LOCUS       DASQDO010000380         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1080963, whole genome shotgun sequence.
ACCESSION   DASQDO010000380 DASQDO010000000
VERSION     DASQDO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080963"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VEV43_10640"
     CDS             <1..637
                     /locus_tag="VEV43_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HYP24022.1"
                     /translation="VVPATEPGKGQALWESLTAAAGDLIVWLDSDTRELSAHFVTNLV
                     APLVGDDSIALCKGFYRRPLGDRGQGGGRVTELAARPLLALLFPELAAVVQPLSGEYA
                     GRAEVLRSLPMFTGYAVEAGILIDLVDRLGLDALAQANLGGRVHRNRDVLALGRMAAE
                     IMQAILTRAEDLGRLKTVDELGDVLVQFEGTGPPLPARVAIGERPPHDESR"
     gene            complement(634..771)
                     /locus_tag="VEV43_10645"
     CDS             complement(634..771)
                     /locus_tag="VEV43_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24023.1"
                     /translation="MDLAHGATILHGDDVVVFAITCAIAALVLGGMWFAGRRRSRKLG
                     R"
     gene            complement(805..2028)
                     /locus_tag="VEV43_10650"
     CDS             complement(805..2028)
                     /locus_tag="VEV43_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYP24024.1"
                     /translation="MSTPAPPRLPAGERLRRAGVAAWSIIGILIVVAIGLWLLYEIRV
                     IFPPLVLALLIIYLLNPLVTRLEERGVRRSVGAVLAYVVVLGGLTVLVVLIAPGISAQ
                     VSNFSESVPELRTDLVDTINDFAVDLDDRFGLEIDTEQISCLLGPEGTSVEGEFSTAE
                     CDRVTREFRESISHHAGQITEIGFSVLEGLLVFILAPLLALYILIDLPHLQRDALNLV
                     PETHRGEFADLGSKIGRAVGGFFRGQLLVALLVGVMSSIGFWIIGLPFWLVIGAIAGF
                     TNLIPLVGPFIGGGLGLIVGTITEGSGLGLLAALVALIVQQIDNHVISPNVMKRTVQI
                     HPATVMLSLLAGGTIAGFWGVLLGVPAVAVVKLLLAHFWTTRVLGEDVSPHARVGGTQ
                     EPPSVVPEETEPGPS"
     gene            complement(2057..3181)
                     /locus_tag="VEV43_10655"
     CDS             complement(2057..3181)
                     /locus_tag="VEV43_10655"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HYP24025.1"
                     /translation="MRILLAPDKFKTTLDATSVAEAMALGVRDTFPDADVRVHPLADG
                     GEGSLDCVAAAGTGSFVTVPAEDSVGAPVTARAFEDGSTVMVAAHETQLLAARPTPAV
                     ALRASSRGLGVVMLAALAAFPGRAVVVWVGGTASTDGGAGAAQAAGWRLLDARGRDVG
                     PGGAELRRLDRVVPPEEPFGARVTGACDVVNTLLGDRGAARVFSAQKGARPADMQLLE
                     DGLERLADCIRTDLGIDVASLPYGGAGGGIGAGLTAFFGADLEDGFMRIANETRLDAA
                     LAWADAVVTGEGRVDAGSLGGKVTSNVARLCKHAGKRCLVVTGEVALPAALESAEEMI
                     GADAVVSTVDRCGRERSFRDPAACVRAVTAELVRERIAPT"
BASE COUNT          519 a         1193 c         1236 g          427 t
ORIGIN      
        1 ggtggtcccg gcgaccgagc ccgggaaggg tcaggctctg tgggagagcc tgacggcggc
       61 ggcgggcgac ctgatcgtgt ggctcgactc ggacacgcgg gagctctcgg cccacttcgt
      121 gacgaacctc gtcgcgccgc tggtcgggga cgactcgatc gcgctgtgca aggggttcta
      181 ccggcgcccg ctgggagacc ggggccaagg cgggggccgc gtgaccgagc tggcggcgcg
      241 gccgctgctc gcgctgctgt tcccggagct cgcggcggtc gtccagcccc tgtcgggcga
      301 gtacgcgggc cgggccgagg tgttgcggtc gctgccgatg ttcaccggct acgccgtcga
      361 ggccggcatc ctgatagacc tcgtcgaccg cctcggcctc gacgcgctcg cgcaggccaa
      421 cctcgggggg cgcgtccacc gcaaccgcga cgtgctcgcg ctcggccgga tggcggccga
      481 gatcatgcag gcgatcctca cgcgggccga ggacctcggg cgtctgaaga ccgtcgacga
      541 gctgggcgac gtgctcgtcc agttcgaggg gacggggccg ccgctaccgg cgcgagtggc
      601 tatcggtgag cgcccgccgc acgacgagtc ccgctagcgg cccagcttcc ggctgcgccg
      661 tctgcccgcg aaccacatcc cgccgagtac cagcgccgcg atggcgcagg tgatcgcgaa
      721 gacgaccacg tcgtccccgt gaaggatcgt cgctccgtgt gcaaggtcca cgcgtgctcc
      781 tcctgtcgaa aaagagatcc cagcctacga cggccccggc tcggtctctt cgggaacgac
      841 cgacggcggt tcctgtgtgc cgccgacgcg ggcgtggggc gacacgtcct cgccgagcac
      901 gcgggtcgtc cagaagtgcg cgagcaggag cttcacgact gccaccgccg ggacgccgag
      961 cagcaccccc cagaagcctg cgatcgtccc gccggcgagc agcgacagca tcacggtcgc
     1021 gggatggatc tggaccgtgc gcttcatcac gttcgggctg atcacgtggt tgtcgatctg
     1081 ctggacgatc agcgcgacga gcgcggcgag aaggccgagg cccgagcctt cggtgatcgt
     1141 ccccacgatc aggccgagac cgccgccgat gaacgggccg acgagcggga tcaggttcgt
     1201 gaagcccgcg atcgccccga tgacgagcca gaacggcagc ccgatgatcc agaacccgat
     1261 gctcgacatc acgccgacga ggagcgcgac gaggagctgc ccgcggaaga agcccccgac
     1321 cgcgcggccg atcttcgagc cgaggtcggc gaactcgccg cgatgcgtct ccggcacgag
     1381 gttcagcgcg tcccgctgca ggtgcggcag gtcgatgagg atgtagagcg cgagtagcgg
     1441 ggcgaggatg aagacgagca gcccttcgag gaccgagaac ccgatctcgg tgatctgccc
     1501 cgcgtgatgg ctgatgctct cgcggaactc gcgcgtgacg cggtcgcact cggcggtcga
     1561 gaactcgcct tcgaccgacg tgccctcggg gccgaggaga cacgagatct gctcggtgtc
     1621 gatctcgagc ccgaagcgat cgtcgaggtc gacggcgaag tcgttgatcg tgtcgaccag
     1681 gtcggtacgg agctcaggga cgctctccga gaagttcgag acctgcgcgg agatgcccgg
     1741 cgcgatgagc acgacgagca ccgtcaggcc ccccaggacg acgacgtagg cgaggaccgc
     1801 cccgaccgag cgccggacgc cgcgctcctc caggcgcgtc acgagcgggt tcaggagata
     1861 gatgatcaag agggcgagca cgagcggcgg gaagatcacg cggatctcgt agagcagcca
     1921 cagcccgatc gcgacgacga tcaggatgcc gatgatcgac cacgcggcga cgcctgcgcg
     1981 gcgcagcctc tcaccggcgg gaaggcgcgg cggcgcgggc gtgctcacgg cgacagtatg
     2041 accggggggc gcgggtctac gtgggcgcta tgcgctcgcg cacgagctcc gcggtgaccg
     2101 ctcgcacaca agccgcgggg tcgcgaaacg acctctcgcg gccgcagcgg tcgaccgtcg
     2161 acacgacggc gtccgcgccg atcatctcct ccgcggactc gagcgcggcc gggagcgcga
     2221 cctcccccgt gacgacgagg cacctcttcc ccgcgtgctt gcagagccga gcgacattcg
     2281 acgtcacctt gccgccgagg ctgccggcgt ccacgcggcc ctcgcccgtc acgaccgcgt
     2341 cggcccatgc gagcgcggca tcgaggcggg tctcgttggc gatgcgcatg aagccgtcct
     2401 cgaggtccgc gccgaagaag gccgtgaggc cggcgccgat gccgccaccc gctccgccgt
     2461 acggcagcga ggcgacgtcg atgccgagat ccgtgcggat gcagtccgcc agtcgctcga
     2521 ggccgtcctc gagcaactgc atgtcggcgg gccgcgcacc cttctgcgcg gagaagacgc
     2581 gggccgcgcc gcggtcgccg agcagcgtgt tgacgacgtc gcacgcgccg gtgacgcgcg
     2641 cgccgaacgg ctcctcggga gggaccacgc ggtcgagccg gcgcagctcg gcgccaccgg
     2701 gaccgacgtc gcggccgcgc gcgtccagca gcctccaccc ggccgcctgc gccgcgccgg
     2761 cgccgccgtc cgtcgacgca gtcccgccga cccagacgac gacggcacgc ccggggaacg
     2821 cggccagcgc ggcgagcatg acgacgccca acccgcgcga cgacgcacgc agggcgacgg
     2881 caggggtcgg gcgagccgct agcagctggg tctcgtgcgc cgcgaccatg accgtcgagc
     2941 cgtcctcgaa ggcgcgcgcc gtgaccggcg ctccgacgga gtcctcggcc gggacggtga
     3001 cgaacgatcc cgtcccggcc gccgcgacgc agtcgagcga tccctcgccc ccgtcggcga
     3061 gcgggtgcac gcgcacgtcg gcatcgggga aggtgtcgcg cactccgagc gccatcgctt
     3121 ccgcgacgga cgtcgcgtcg agcgtcgtct tgaacttgtc gggcgccagg aggatcctca
     3181 agtccaccta ggatacggag catgcgagac gacacgacta tcgtgctcgt gtcgaatcgt
     3241 ggtccggtgt ccttcgtcga ggccggcggc gggttcgaga cgaaacgggg ggccggaggg
     3301 ctcgccggcg cgctcgaccc ggtcgcgcgc cgcctcggcg agcgcgcggt gtgggtcgcc
     3361 gcggcgacgt cggac
//
LOCUS       DASQDO010000381         9097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_40281, whole genome shotgun sequence.
ACCESSION   DASQDO010000381 DASQDO010000000
VERSION     DASQDO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9097
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40281"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1263)
                     /locus_tag="VEV43_10660"
     CDS             complement(136..1263)
                     /locus_tag="VEV43_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24026.1"
                     /translation="MKLVKLLPAVALLLGILPGPPAGALGAASPNVEYVTTVPLEAGT
                     WTTGKLHDGYFYASGMKSFTIYDVSDPASPQLVSHTPTGVQFINEDIDTNGEILLVTD
                     ERLRGILQVWDVSDKAKPVKLAELEGMVDHTFACVLDCTWAYGGGGDIVDLRDPANPK
                     PAGRWHGEMPPLWGFDTYEVSPGVVVTGSKVMYLLDGRKDPANPKVLAQGTTADQRTI
                     HSLKWPRAGKDKFMLVQSETSPKPQCDDRSGKFMTWDMTQWRKTKTFTMIDEYLPPAG
                     NWVDANPGASALGCSATWFHHHPSFRNGGLVAGAFFDHGTRFLWVDDAGQIEEVGHYM
                     PYGGSTTSTYWVTDEIVYSVDITHGIDVLRFTGLDDLKEER"
     gene            1419..1964
                     /locus_tag="VEV43_10665"
     CDS             1419..1964
                     /locus_tag="VEV43_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24027.1"
                     /translation="MTTGDQTMGGTGTLTAEEQRDLARCVSLVLEVGILERSSSAGSD
                     AAAERVASLVGESQELAPRLEEIFTSHAGTLRSAYEAAASGTDAAQAEGPALSPEARE
                     RLVQLVQAEGSGDVVAAAVGMARQAAAMPAPDAALRDDVGVPNVVHHVLQGLALASSL
                     TLGPEVGAVFEVIDIIFHLFG"
     gene            complement(1970..2923)
                     /locus_tag="VEV43_10670"
     CDS             complement(1970..2923)
                     /locus_tag="VEV43_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYP24028.1"
                     /translation="MRLYRYTSNRGGFQPRLAVAVGDGEPVDLLNAWDEVTGTRLERE
                     SAQFLVSTALLIEQGPELWKEVRDVARAAQSLPWLEVTATERNLRWLCPIDKVASLKD
                     FLAFEDHVKRGAQRRGASVPEYWYEAPVYYKGNHRAVIGPEATCPWPSYTNRLDFELE
                     LALIVGRRGRDVSREVAGDYVFGFTIFNDFSARDVQAKEMSAWLGPAKGKDFGNALGP
                     CIVTADEVGTEPDLEMICRVNGEEWGRARSSDAKWRWADILAHVSEGEDIWPADVYGS
                     GTPGGCCGLDQGRELSPGDVVELEIEKIGVLRNTVGKPGAR"
     gene            complement(2923..4041)
                     /locus_tag="VEV43_10675"
     CDS             complement(2923..4041)
                     /locus_tag="VEV43_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HYP24029.1"
                     /translation="MEFVFSKGVVTKQAHVSIPEGMYEEEHGREAFAGRASHLYRKHP
                     PTAWTRIDGPLRPRALNLNEMKPEDWVEANATWQLIAGNDDVRLYLSRRAEPMPYFLR
                     DADGDVCYFVHRGSGVIETDYGPLDYRQGDYVIVPKGTTHRVVPAGGESFFFVIEGEG
                     EYRIPERGLMGRHAQFDPGVLVVPEPDPHDEEGEFEVRVKRNGEFSSLWFQWHPLDVV
                     GWQGDLCPVKLNILDHRPVYSPSYHMPPSAHCTFENDAFVICSFVPRPLEEDPEVLKV
                     PFYHANIDADEVLFYHSGNYFSRAGIDMGYMTLHPQGVHHGPQPAAIEAAKTKERTDE
                     IAVMVETEHPLQLSPEAEQVVVTEYETSWARGMGLLDE"
     gene            complement(4041..5138)
                     /gene="hppD"
                     /locus_tag="VEV43_10680"
     CDS             complement(4041..5138)
                     /gene="hppD"
                     /locus_tag="VEV43_10680"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HYP24030.1"
                     /translation="MTERHDPFPILGFDYVQFFVGNAKQAAHFYRAMGFTPVAFVGLE
                     TGSRDRVSYAVRQGEVTFVFTGALHPDSPIADHVHRHGDGVHDVALRVPDAEEAFRVA
                     VERGAKAVSEPEVFEDENGKVVRAAIGTYGETIHSLVQRDNYSGTFFPGFKDLPEAGD
                     TKGFGLHYVDHVVGNVELGKRDEWVSFYEKVMGFVELKHFSDDDISTEYSALMSKVVW
                     DGEGKIKMPINEPATGKRKSQIEEYLEYYGGPGVQHVAMTSTDIVSTVESMRAAGIGF
                     LKIPDNYYDDAVKRVPEIADQVEALQRNGILVDKDEEGYLLQIFTHIAQDRPTVFYEV
                     IERHGARGFGEGNFKALFEAIEREQAARGNL"
     gene            complement(5193..5687)
                     /locus_tag="VEV43_10685"
     CDS             complement(5193..5687)
                     /locus_tag="VEV43_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24031.1"
                     /translation="MGLALTLLAVPSGADTTWVTDRDDTKGFLDVKAIAHGHGRGDRL
                     KHTVTTFAKWRSKDLWCGVVAMEFPGLDRELIVFYRKKLRAIVVNERTGEGIGNPRVT
                     RPTRRRVAVAFPRRWLRPGLDEYRWFVRTVRRGSDCPARDGDKSVVVRDRAPRRGRSI
                     LHRL"
     gene            complement(5756..6940)
                     /locus_tag="VEV43_10690"
     CDS             complement(5756..6940)
                     /locus_tag="VEV43_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24032.1"
                     /translation="MRLSNSRKVWPGLLVVAVTVACGAPSAQEPRSALAQRSTETHAA
                     YVLDANGESLTSRIVRVDPETQRVEGSYPAGYDPVMTVSPDGTRLYVASARNRGGKLR
                     DELAVIDTVGGKVLSTTPVEDRWEGAGVSPHPSLSLSSDGLWLYVLQVRLTGGAGAEY
                     SVATFDTTRREFLPESVALGSCGAGFLVPSAHDGGRRLQIVCPETHDVRVLTVASNGS
                     ADSAETIDLPRVDDGRTDANRNPLELWRLSSAVGSPSGDELYAITQNGRVFTVDLLTE
                     EVTASTDLELGPDTFVPIGQGAAGGDRLFLGAASLDSTAGIDRLRASHVLVVDAAEGV
                     AHAAATEGAFEALGASPLGDAIFVVDSEGQTVTVIDADDPSERAVVRNVGNTPSRIEV
                     VR"
     gene            complement(6964..7383)
                     /locus_tag="VEV43_10695"
     CDS             complement(6964..7383)
                     /locus_tag="VEV43_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24033.1"
                     /translation="MALLFAADEAGLLRVPHPQLHRQVSQRRRRHAPPTVTALRYGVE
                     LGAGLTTFVPVTTFYVLLTAVFFVGDPVMGAGVMAGFAGARAAPVAAIAIGSPDMATA
                     TRAGDSLLGWEPLVHLVNALVLAGASAYLLTSSASNP"
     gene            complement(7588..7770)
                     /locus_tag="VEV43_10700"
     CDS             complement(7588..7770)
                     /locus_tag="VEV43_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24034.1"
                     /translation="MQSVRTTVLPSRLRRILLLAVAGLGGSAVALGTSEGTGAGLTPV
                     AVVLATIAGFSALWSP"
     gene            complement(7773..8333)
                     /locus_tag="VEV43_10705"
     CDS             complement(7773..8333)
                     /locus_tag="VEV43_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HYP24035.1"
                     /translation="MDLDERQRREALEEGGSFDRFVSRGLADASQRLSRRGVLAKAGK
                     VVLGVLGVSTGLAALPLDRAGADSDTEIIGGCDDWKLCGLWGRLCNCCNGTAGIGTCP
                     GCADLNPGHFWTSCCCKPSANTMYWIRYYDCCNGCPSSKVDECKTCRTCWKNPVEQPE
                     WCYTTGVGTGQYVCTTVVKMNSCGMC"
     gene            complement(8364..8783)
                     /locus_tag="VEV43_10710"
     CDS             complement(8364..8783)
                     /locus_tag="VEV43_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24036.1"
                     /translation="MRGKQTLLVFLSTGCDSCDDVAPAVRSIARSESGQTETIVVTPG
                     EADSYDSDFFRRHKLDDLPIVVSQDLAAELGVMTTPYALLVDETGVLRTKGIVNNLDQ
                     LVSLFNAVEVGYPSMDAYMTARTRGSSDEKESVAADV"
     gene            complement(8795..8995)
                     /locus_tag="VEV43_10715"
     CDS             complement(8795..8995)
                     /locus_tag="VEV43_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24037.1"
                     /translation="MNGLWIASYVALWVVVILLAVAVVALGRQIGLLHYRLPPSVARM
                     GAPGPKVGDRVDPLEEILPGGP"
BASE COUNT         1474 a         3151 c         2994 g         1478 t
ORIGIN      
        1 cgacggcgaa gtacgcggcg cagacgacct cgccgcccgg cagctcggtc tggcacgtcc
       61 cggccttcgc cggcgccgcc ggcaacagcg tcgccgcggc taccgcaccc gccactgcca
      121 tcatcctgat cctcttcatc gctcctcctt caggtcgtcc agtccggtga aacgaagtac
      181 gtcgatcccg tgcgtgatgt cgaccgagta cacgatctcg tcggtgaccc agtacgtcga
      241 ggtggtcgag cccccgtacg gcatgtagtg gccgacctct tcgatctggc ccgcgtcgtc
      301 gacccacagg aacctcgttc cgtggtcaaa gaacgcgccg gcgacgaggc caccgttccg
      361 gaacgacggg tgatggtgga accaggtcgc cgagcagccg agcgcgctcg cccccgggtt
      421 cgcgtcgacc cagttccccg cgggcggcag gtactcgtcg atcatcgtga acgtcttggt
      481 cttgcgccac tgcgtcatgt cccacgtcat gaacttcccg gagcggtcgt cgcattgcgg
      541 cttcggcgac gtctcgctct gcacgagcat gaacttgtcc ttgcctgcgc gcggccactt
      601 gagggaatgg atcgttcgct ggtcggccgt cgtgccctgc gcgaggacct tcgggttcgc
      661 cgggtccttg cggccgtcga gcaggtacat gaccttcgag cccgtgacga cgacgccggg
      721 cgacacctcg taggtgtcga agccccacag cggcggcatc tcaccgtgcc agcgaccggc
      781 cggcttcggg ttcgcagggt cgcgcaggtc gacgatgtcg ccgccgccgc cgtacgccca
      841 cgtgcagtcg agcacgcacg cgaacgtgtg gtcgaccatg ccctcgagct cggcgagctt
      901 caccggcttc gccttgtcgg acacgtccca gacctggagg atcccgcgca ggcgctcgtc
      961 cgtgacgagc aggatctcgc cgttcgtgtc gatgtcctcg ttgatgaact ggacgccggt
     1021 cggagtgtgg ctgacgagct gcgggctggc ggggtcggag acgtcgtaga tcgtgaagct
     1081 cttcatcccc gacgcgtaga agtagccgtc gtgaagcttg ccggtggtcc aggtcccggc
     1141 ctcgaggggg accgtggtca cgtactcgac gttgggactg gcggcgccga gcgcgcccgc
     1201 aggggggccg gggaggatgc cgagcaggag ggcgacggcc gggaggagct tgacgagctt
     1261 catgcgacga gggttcgccg tgagtagagc cgcctccgtc ccccaggtgg gtgaaatcgg
     1321 tgaagtgttc gctacctaac ggacgccttt acgctctggc cccatcctgg ttgcaccagg
     1381 acggcggccg cgggagcggt cgcggggagg aggaagacat gaccaccggt gaccagacga
     1441 tgggcggaac cgggacgctg acggccgagg agcagcggga tctcgcgcgg tgcgtctcgc
     1501 tcgttctcga ggtcgggata ctcgagcgca gctccagcgc ggggtcggac gcggcggcgg
     1561 agcgcgtcgc ttccctcgtc ggcgagtcgc aggagctggc gccgcgcctc gaggagatct
     1621 tcacgtcaca cgccggcacg ctgcgctcgg cttacgaggc cgccgccagc ggaacggacg
     1681 ccgcgcaggc ggagggcccg gcgctgtcgc ccgaggcgcg ggagaggctc gtgcagctcg
     1741 tgcaagccga gggctcgggc gacgtcgtcg ccgccgcggt cggcatggcg cgtcaggccg
     1801 ctgcgatgcc ggcgccggac gccgccctac gcgacgacgt cggcgtcccg aacgtggtcc
     1861 accacgtgct gcagggtctc gcgctcgcgt cgtcgctgac gctcggtccc gaagtcggag
     1921 cggtgttcga ggtcatcgac atcatcttcc acctcttcgg ctgaggcggt cagcgggccc
     1981 ccggcttgcc gaccgtattt cgcaggaccc cgatcttctc tatctcgagc tccactacgt
     2041 cgcccgggct cagctcgcgg ccctggtcca ggccgcagca gccgcccggg gttcccgagc
     2101 cgtatacgtc ggccggccag atgtcctcgc cttcggagac gtgggcgagg atgtctgccc
     2161 agcgccactt cgcatccgaa gagcgcgccc ggccccattc ctcgccgttg acgcggcaga
     2221 tcatctcgag gtccggctcc gtgcccacct cgtccgccgt cacgatgcag gggccgagcg
     2281 cgttcccgaa gtccttgccc ttggccgggc cgagccacgc gctcatctcc ttcgcctgca
     2341 cgtcgcgggc cgagaagtcg ttgaagatcg tgaagccgaa gacgtagtca ccggcgacct
     2401 cacgcgacac gtcgcggccc cggcgtccga cgatcagcgc gagctccagc tcgaagtcga
     2461 gccggttcgt gtacgacggc cacgggcacg tcgcctccgg gccgataacc gcgcggtggt
     2521 tccccttgta gtagacgggt gcctcgtacc agtactcggg gaccgatgcc ccccgccgct
     2581 gcgcccctcg cttcacgtgg tcctcgaacg cgaggaagtc cttcagcgac gcgaccttgt
     2641 cgatcgggca gagccaccgg aggttccgtt cggtcgcggt gacctcgagc cacggtaagg
     2701 actgcgcggc gcgtgcgacg tcgcgcacct ctttccacag ctcggggccc tgctcgatca
     2761 agagcgcggt cgagacgagg aactgcgcgg actcccgctc gagacgcgtg ccggtcacct
     2821 cgtcccacgc gttgagcagg tcgacgggtt caccgtcgcc gaccgcgacc gcaagacggg
     2881 gctggaaacc tcccctgttc gacgtgtacc tgtagaggcg cactactcgt cgagcaaccc
     2941 catgccgcgc gcccacgacg tctcgtactc ggtgactacg acttgctcgg cctccggcga
     3001 gagctgcagc ggatgctcgg tctcgaccat gaccgcgatc tcgtccgtgc gctccttcgt
     3061 cttcgctgcc tcgatggccg cgggctgggg gccgtggtgg acgccctgcg gatgcagcgt
     3121 catgtagccc atgtcgatgc cggcccggct gaagtagttg cccgagtggt agaagagcac
     3181 ctcgtccgcg tcgatgttgg cgtgatagaa gggcaccttc aggacctcgg ggtcctcctc
     3241 gagcggccgc ggcacgaacg agcagatcac gaaagcgtcg ttctcgaacg tgcagtgagc
     3301 cgacggcggc atgtgatagc tcggcgagta caccggccgg tggtcgagga tgttgagctt
     3361 caccgggcac aggtcgccct gccacccgac gacgtcgagc gggtgccact ggaaccacag
     3421 cgacgagaac tcgccgttgc gcttcacgcg tacctcgaac tcgccctctt cgtcgtgcgg
     3481 gtcgggctcg ggaacgacga gcacgcccgg gtcgaactgc gcgtgccgtc ccatcaggcc
     3541 gcgctcgggg atgcggtact cgccttcgcc ttctatgacg aagaagaacg actcgccgcc
     3601 cgccggcaca acgcggtgcg tcgtcccctt cggcacgatc acgtagtcgc cctggcggta
     3661 gtcgaggggg ccgtagtcgg tctcgatcac gcccgagccg cggtgcacga agtagcagac
     3721 gtcaccgtcg gcgtcgcgca ggaagtacgg catcggctcg gccctgcggc ttaagtacag
     3781 cctgacgtcg tcgttgccgg cgatgagctg ccacgtcgcg ttcgcctcga cccagtcctc
     3841 gggcttcatc tcgttgaggt tcagagcgcg ggggcggagc gggccgtcga tcctcgtcca
     3901 cgccgtcgga ggatgcttgc ggtacaggtg ggacgctcgc cccgcgaacg cctcgcggcc
     3961 gtgctcctct tcgtacatcc cctccgggat cgatacgtgc gcctgcttgg tgacgacgcc
     4021 cttcgagaag acgaattcca ctagaggttc cccctcgctg cctgctcgcg ctcgatcgcc
     4081 tcgaacagcg ccttgaagtt gccttcgccg aagccgcgcg cgccgtggcg ctcgatgacc
     4141 tcgtagaaga ccgtcggccg gtcctgcgcg atgtgggtga agatctggag caggtagccc
     4201 tcctcgtcct tgtcgacgag gatcccgttg cgctgcaggg cctctacctg gtcggcgatc
     4261 tccgggacgc gcttgaccgc gtcgtcgtag tagttgtcgg ggatcttcaa aaagccgatg
     4321 ccggccgcgc gcatcgactc gacggtcgag acgatgtcgg tcgacgtcat cgcgacatgc
     4381 tggacgcccg gacccccgta gtactcgagg tactcctcga tctggctctt gcgcttgccg
     4441 gtcgcgggct cgttgatcgg catcttgatc ttgccctcgc cgtcccacac gaccttcgac
     4501 atgagagccg agtactccgt cgagatgtcg tcgtcggaga agtgcttgag ctcgacgaag
     4561 cccatgacct tctcgtagaa cgagacccac tcgtccctct tgccgagctc gacgttgccg
     4621 acgacgtggt cgacgtagtg gaggccgaag cccttcgtgt cgccggcctc gggcaggtcc
     4681 ttgaagcccg ggaagaacgt gcccgagtag ttgtcgcgct ggacgagcga gtggatcgtc
     4741 tcgccgtacg tcccgatcgc ggcccggacg accttgccgt tctcgtcctc gaagacctcg
     4801 ggctcggata cggccttcgc cccgcgctcg accgcgacgc ggaacgcctc ctcggcgtcg
     4861 gggacgcgca gcgcgacgtc gtggaccccg tcgccgtgac gatggacgtg gtccgcgatc
     4921 gggctgtcgg gatggagcgc cccggtgaag acgaacgtca cctcgccctg acgaacggcg
     4981 taagacacgc ggtcgcggga gccggtctcg aggcctacga aggccaccgg cgtgaagccc
     5041 atcgcccggt agaagtgagc ggcctgcttc gcgttgccga cgaagaactg gacgtagtcg
     5101 aacccgagga tggggaacgg gtcgtgtcgt tcggtcacct aaccacctcc tgagatcagg
     5161 gggtcaggat agcggcccgg cgacgcgggc ggtcagaggc gatggaggat gctgcggccc
     5221 ctgcggggag cgcgatcgcg gaccacgacg ctcttgtctc cgtcgcgcgc ggggcaatcg
     5281 cttcctcgcc gaaccgtgcg aacgaaccac cggtactcat cgagcccggg ccggagccat
     5341 ctccggggga aggcgacggc cacgcggcga cgggtggggc gggttacccg cggattcccg
     5401 atcccctcgc cggtccgctc gttgaccacg atcgctcgca gcttctttct gtagaagacg
     5461 atcagctctc gatcgaggcc cgggaactcc atcgcgacga cgccgcacca gaggtccttc
     5521 gatctccact tggcgaaagt cgtaacggtg tgcttgagcc gatcgccgcg accgtgaccg
     5581 tgggcgatgg ccttgacgtc gaggaacccc ttcgtatcgt ctcggtcggt cacccacgtg
     5641 gtgtccgcgc ccgaaggaac ggcgagaagc gtgagggcaa gacccacggc gagaaccgcc
     5701 gatgaccacc tggcgacagc gcgcattatg ctcgtcctct cccgatcgac cggagtcatc
     5761 gcactacctc gattctgctc ggggtattgc ctacgttccg aacgaccgcg cgctctgaag
     5821 ggtcatcggc gtcgatcacc gtcacggtct gtccctcgct gtccaccacg aagatggcgt
     5881 ccccgagcgg cgacgctccg agagcttcga aggcgccctc ggttgccgct gcatgcgcga
     5941 ctccctccgc ggcgtcgacg accagcacgt gcgacgccct gagacggtcg atcccggcag
     6001 tcgagtcgag cgaagcggcg cccaggaaca ggcggtcgcc accagcggcc ccttggccta
     6061 tcggaacgaa ggtgtccggc cccagctcga ggtccgtcga cgccgtcacc tcctcggtca
     6121 gcagatcaac cgtgaaaacc cggccgttct gcgtgatcgc atagagctcg tcacccgaag
     6181 gcgacccgac tgccgagctc agacgccaca gctcgagcgg atttcggttc gcgtccgtgc
     6241 gaccgtcgtc gacccggggc agatcgatcg tttctgccga atccgcagat ccgttagagg
     6301 cgaccgttag gacgcggaca tcgtgtgtct cgggacagac gatctgcagg cggcgaccgc
     6361 cgtcgtgggc ggacggaacg aggaaccccg cgccgcaaga gccgagagcg acggactccg
     6421 gcaggaactc acggcgcgtc gtgtcgaacg tggcgacgga atactcggcc cctgcgccgc
     6481 ctgtgaggcg aacctggagg acgtagagcc agagcccgtc agatgacagg gaaagagacg
     6541 gatgaggcga gacgccggca ccctcccacc gatcctcgac cggcgtcgtt gacaggactt
     6601 tgccgccgac ggtgtcgatc accgcgagct cgtcgcgcag cttcccgccg cggttcctcg
     6661 ccgaggctac gtacagcctc gttccatctg gtgacaccgt catgacgggg tcgtaccccg
     6721 cgggatacga accctccacc ctctgtgtct cgggatccac tcggacgatc cgcgacgtca
     6781 ggctctcgcc gttcgcgtcg agcacgtacg ccgcatgggt ttccgtcgaa cgttgggcga
     6841 gcgccgatcg cggctcttgt gcggacggcg cgccgcaggc cacggtgacg gcgacgacca
     6901 ggagccccgg ccagaccttc ctcgagttgg acaagcgcat ggcaatcgct cctctccggt
     6961 tgctcatggg ttcgacgcgc tagaggtcaa gagatacgcg ctcgctcccg caaggaccaa
     7021 ggcgttgacg agatggacga gcggctccca gcccagcaac gagtctcccg cccgagtagc
     7081 cgtcgccata tcgggggatc cgatcgcgat agccgccacg ggggccgcgc gagcaccggc
     7141 aaaacccgcc atcacgcccg cccccatcac cgggtcgccg acgaagaaga cagccgtcaa
     7201 caacacgtag aacgtcgtca ccggaacgaa cgtcgtgagc cccgcgccga gctcgacccc
     7261 gtaacgaagc gcggtgacgg tgggcggcgc gtgccgtctg cggcgctggc ttacctggcg
     7321 gtgcagctgc gggtgcggga cgcgcagcaa tcccgcctcg tcggcggcga agagcagcgc
     7381 cattgcgccc aggatcggca agccaacaga cggctggccg tcgccccaaa aggagtgttg
     7441 cgtccatcgt ccggcgaggg acaacgacgc tccaacgacg accccgcccg agacggcacc
     7501 caccgcgtgg gcgaccaccg gtccgagatg cttccgcgac gaacccccgt gcacgacggg
     7561 gaggatggtc ccgaccatcg aaaggcctca cggcgaccac agggccgaaa atcccgcgat
     7621 ggtcgcgagc acgaccgcga ccggcgtcag accggcgccc gtaccttctg acgtcccgag
     7681 cgctaccgcg gagccgccca ggccggcgac cgccagcagc aggatccggc gaagccggct
     7741 cggcagaacg gtcgtgcgga cagactgcac cgttagcaca tcccgcacga gttcatcttg
     7801 acgacggtcg tgcaaacgta ctgacccgtg cctactccgg tcgtgtaaca ccactccggt
     7861 tgctcgaccg ggttcttcca gcacgtgcgg caggtcttgc actcgtccac cttcgaagat
     7921 ggacacccgt tgcaacagtc gtaatagcgg atccagtaca tcgtgttagc cgacggtttg
     7981 cagcagcagg aggtccagaa gtgccccggg ttcaggtcgg cacagccggg acacgttccg
     8041 atgcccgcag taccgttgca gcagttgcac aggcggcccc acaacccgca tagcttccaa
     8101 tcgtcacagc cgccgatgat ctccgtgtcg ctgtccgcgc ccgcccgatc gagcggcagg
     8161 gccgcgagac ccgtggacac accgagcacg cccagcacga cctttccggc tttcgcgagc
     8221 acgccccggc gcgataggcg ctgcgacgca tccgcgagcc cgcggctgac gaaccggtcg
     8281 aacgatccgc cttcttcgag cgcttcccgg cgttgcctct cgtccagatc catgcatccc
     8341 tccacccggc gatgccgcgg gtgtcacacg tccgcggcga ctgattcctt ttcgtccgac
     8401 gatccgcgcg ttcgagccgt catgtacgca tccatgctgg ggtagcccac ttccaccgcg
     8461 ttgaacagac tcacgagctg gtcgaggttg ttgacgatcc ctttggtgcg gaggactccc
     8521 gtctcgtcga ccaacaacgc gtaaggagtc gtcatcacgc caagctccgc ggcgaggtcc
     8581 tgggagacca cgatcggcag gtcgtccagc ttgtgtcgcc ggaagaagtc tgagtcgtac
     8641 gagtcggcct cgccgggggt gacgacgatg gtctcggtct gcccgctctc gctccgggcg
     8701 atcgaccgca ccgcaggagc gacgtcgtcg cacgagtcac atcccgtcga caggaacacc
     8761 aggagggtct gtttccctcg cacggatcca agggtcacgg tccgcccggc aatatttcct
     8821 cgagcggatc cacgcggtcg cccaccttcg ggcccggggc ccccatccgc gcaaccgacg
     8881 gcggcaagcg ataatgcagg aggccgatct ggcgccccaa cgcgacgacg gcgaccgcga
     8941 gcagtatcac caccacccac agggccacgt acgaggcgat ccacaagccg ttcatccgtg
     9001 agtctccctt ccgaagccga tgcgcagaag tcgcggcgac gcctgaggat gggccgctcc
     9061 gtcgccgggc gtcgcagcca tcctcggaga cgggctc
//
LOCUS       DASQDO010000382        17273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_958441, whole genome shotgun sequence.
ACCESSION   DASQDO010000382 DASQDO010000000
VERSION     DASQDO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17273
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958441"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VEV43_10720"
     CDS             <1..292
                     /locus_tag="VEV43_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24038.1"
                     /translation="SRGVLAAALVTLDDAATRLGRVVSRNDSHIEAELDDLAVLLDAV
                     EDTREDLRGALRALPEMLVAVERANSYGQWTNIHLVHLCKDDTGECGRRWMP"
     gene            289..1383
                     /locus_tag="VEV43_10725"
     CDS             289..1383
                     /locus_tag="VEV43_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYP24039.1"
                     /translation="MRSFRERRAWLVGITCIVLIAAGVTAAFSINRFQGLRGVYTLSA
                     DVADAAGLQSGNEVRVAGVKVGTVKRVVLTPDAARVDMEIQDDVRIPVESSLEVKLKT
                     LLGQKFVDLQMPRAYVEASSAGTDPDDATEGYFEAGAVIPLDQTSVPFEIYEAANEGT
                     DVLAQIDKQALRELIEVLDETVGASKEELGRALDAVGRAGDVVARNSAPLKRLLRQSA
                     DLTGTLDAAGSDIDGILLRSADVLEVLAERRGTISSLLAATEDLSRNLGVLIRASKGS
                     IEIGAADLNSVLLLAQGELATIERSLAELGTAQEMFGQPLRFGRFVEGHICSITTADT
                     CIPDGSPEDPGLPVHGTEPGSTHTVDRRHQ"
     gene            1380..2660
                     /locus_tag="VEV43_10730"
     CDS             1380..2660
                     /locus_tag="VEV43_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HYP24040.1"
                     /translation="MRARLISLLTTLALVAAGVATYTLTRTEAATYTVTADVAQAPNL
                     FEGGRVAVRGIDVGEIVDVEPRGSAVRVTMEIDESVTVPADARLSIVPITVIADRYVQ
                     LDPPYESGPVLADGDHIDMDRTTIPAELDDVLTQLEGLLSALELEPGETNGPLARLVR
                     NLDTALEGRADSLQRGLKGSAAGLETLASSEQDIRRLIANLDSLFIVLAQRSSEIGLV
                     NERFRSVAESLADDQRALEGTIENLAFLSREGASLIETSGDELGVSFGRLRDVADAIL
                     RHRDQLEKGARWTNAIAQALGETDASGRGRWAYSGRQAPPGTERASYNYRLDSRDTIS
                     CERLNEIVRTVLILTPQAPMDDLVATVNSHLPDEYEDDLEFLVRQLIPVCTDWQAPAV
                     SARTERLVKRVAADVGEKRFVKMLSRWFWEGGPR"
     gene            2657..3667
                     /locus_tag="VEV43_10735"
     CDS             2657..3667
                     /locus_tag="VEV43_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYP24041.1"
                     /translation="MKLLRIAALLAVAVLGSSCALMTTDPSPEVVAYFEDVGDLVEKG
                     GVQVADVEVGSVQEIGLVLEDGRMLARVTMSIDEDERIPAEGLRAVVRQTSLLGEQFV
                     ELIPGPPGAPYIGGDEVTIPVEDTDRIVDVETFLGDLSAFVGGGGLEDLNSFTHAQAL
                     ILEERGDRLGRTIEELEKFTSVLAGRRFDVEAAIDHLASAGSTLAANRDTLGSFLDSL
                     EEANALLAEQGGSLRRLFTSLRRFGTVNSRFLVRHEDAIHRQFRALRPILGGLASAEG
                     ELRVDIAQLRTFLELFPKSIGGGPGGDGKGDYVQVEAVLCEALSACNTKGEKGDVPGE
                     GS"
     gene            3664..4983
                     /locus_tag="VEV43_10740"
     CDS             3664..4983
                     /locus_tag="VEV43_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYP24042.1"
                     /translation="MRLSPRLAINLGVVLLLFVVTVGWVITSVVGPQVTGGQFEVTAD
                     FAESGGVFTNQEVTYRGVLVGRVGDLELNEDGVDIELLIDGEWEGRIPADSIARIQSK
                     SPVGEQFVNLTPGSSAGEMLEDGDAIARANTELPVDFQRLLSALDRVLADVPPEASRR
                     VIQELAGGLRGRGDDLATILASLGTLADTFADVAPEQQRLLDNATGAGAEFLRTREAF
                     AAALRAADQVAAGIGDEPEELRALFASNDRVARAGIRLLARRGDDFESGIGALSDIVA
                     FQLEQRAQIEGMLEYLPGFLHAVEDASVPWRSPDGRKFYRIRVGLILDNQPSSWPCKY
                     ELPNDWERFAFQRDVRPVRTSMDCIPGEDEAAQRSVRALVRSLRSWAKSNPLPEPEAE
                     PKAETKSAEAGLDEVPGEAPVGEAEGAETTFEEATRYFPADWTEPNP"
     gene            5046..5315
                     /locus_tag="VEV43_10745"
     CDS             5046..5315
                     /locus_tag="VEV43_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HYP24043.1"
                     /translation="MRAKQNGTKPVDVVEVMPLGGYRVALVFSDGHRAERDLGPYLVG
                     PVFEPVRDPDYFRRVRVDAQSGTIVWPNGADLAPDSLRMQPAPRD"
     gene            5365..7236
                     /gene="uvrC"
                     /locus_tag="VEV43_10750"
     CDS             5365..7236
                     /gene="uvrC"
                     /locus_tag="VEV43_10750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HYP24044.1"
                     /translation="MAVKRTLQRPPAGTIPARPGAYLFRDAQGRVIYAGKAKSLRSRL
                     SNYFASDLHPRTQAMVEAAADLEWIVTESEVQALHLELNLIKQHRPRYNVRYRDDKTY
                     PYLAITLDEEYPRARVLRERKRKGVRYYGPFAHAYAIRETLDLLLRTFPMRTCSQGVF
                     DRCRRRGKPCLLFHIERCAGPCVEAVSEEEHRRIALELCDFLDGNTKPVTRRLETAMK
                     EAATTEEFELAAKYRDQLDNVRKAIAKQQMVSQQSENLDVIGFAEDELEAAFQVFFVR
                     GGRVTGRKGFVVDKVEDLDTPALVEGFLERLYSEAEPPPHVLVPVEPEDARLIERWLT
                     DLRGSNVKVRVPQRGGKRMLLETVTENAKQAFTQHRLKRASDFAARSQQLTELQEALG
                     MDDAPLRIECFDISNTGPTEAVGSMVVFEDGLPKRSDYRRFAIKWAQGQDDFANMGEV
                     IRRRFARFLEEQENVLAPEGKRAKFAYPPNLVVIDGGKGQLNRAVEVMGELGVDGVTT
                     VSLAKRMEEVFVPGEAEPILIPRGSEALYLLQRIRDEAHRFALTYHQLRRSKKMTQSA
                     LDGIPGLGEVRRKKVLKHFGSVKRVREASLEELRSVPGLPATVAEAIYDNLHGRKAS"
     gene            7233..8126
                     /gene="rapZ"
                     /locus_tag="VEV43_10755"
     CDS             7233..8126
                     /gene="rapZ"
                     /locus_tag="VEV43_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HYP24045.1"
                     /translation="MTAARDVEVTVISGLSGAGMSEAAKAFEDLGWFVIDNLPPALIG
                     KMLSLALGPGHEIKRMALVIDSRGGMFFSEIKDALAELRRDVGSFRLVFLEADDEALV
                     RRFDATRRRHPLALQDHLTVGIQRERKLMLSLRDGADLIIDTSDLSVRDLRRKLSAYF
                     DVTSAAEGLKTTIISFGYKFGLPLDADLVLDVRFLPNPHWVPGLREHTGLDEPVRDYV
                     LGKDVTGDFLARAKDLFAVLLPGYESEGRHYLTIAVGCTGGRHRSVVLAEELGAFVQE
                     KGYPAKVIHRDVERPPAVTAP"
     gene            8123..8998
                     /gene="yvcK"
                     /locus_tag="VEV43_10760"
     CDS             8123..8998
                     /gene="yvcK"
                     /locus_tag="VEV43_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HYP24046.1"
                     /translation="MKVTGIGGGHGLAATLMAARHYADEISAVVTVADDGGSSGQLTR
                     ELGIPPPGDIRNCLVALAGDDERARLYQHRFQSGALTGHTVGNLVIAAFTEMTGDFAE
                     AVAAAGRMLGCEGRVHPATNEMVTLMAQVRDGVVRGQVAVAQTKDPIQALFLEPRSPR
                     ANRDAVQAILDADQVVLGPGSLFTSLIATILVPGIRKALQETAARRVFVCNARMQKGE
                     TEGLDAAAHLESLLAHAGARSVDVVVVQSPVVPVDGVDVTSVKSLGVRVVEADVASES
                     GAHDAERLAAVLASL"
     gene            9066..10073
                     /gene="gap"
                     /locus_tag="VEV43_10765"
     CDS             9066..10073
                     /gene="gap"
                     /locus_tag="VEV43_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HYP24047.1"
                     /translation="MTTRIGINGFGRIGRNFFRALKAKGSSLELVAVNDLGDAETMAH
                     LLKYDSVHGRFGREVKVSNGGFTIDGQDIKLLSERDPAALPWGDLGVDIVIESTGFFT
                     KRGDAGKHVEGGAKKVIISAPAKDEDVTVVMGVNHDKYDPANHHIISNASCTTNCVVP
                     MTKVLQDNWGVDKGFMTTCHAYTNDQNTLDLARDDLRRARAAAVNIIPTTTGAAKAAS
                     LAMPELKGRLDGLALRVPVPDGSITDIVAVLNGEPTKDEVNQAYREAAASAGMQGILE
                     YTEDAIVSSDIVGNSHSCIIDGASTMVNGNMVKVLGWYDNEWGYSNRLVDLTELVASS
                     L"
     gene            10155..10682
                     /locus_tag="VEV43_10770"
     CDS             10155..10682
                     /locus_tag="VEV43_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24048.1"
                     /translation="MRLRTVTDEEDLIAFCRAQHGRLVGLLSLYCGDAAVAEELAQDA
                     LARVCRDWRRIRKMDHPPAWASRIAINLANSYFRRRSAERRARDRLASRPAPDLGALD
                     TAAALGVRQAVAALPPRQRTALLLHYFCDLPFARVAELMGAPEPTVKSLARRGIARLR
                     SPELLGNPEEAPNAV"
     gene            10672..12000
                     /locus_tag="VEV43_10775"
     CDS             10672..12000
                     /locus_tag="VEV43_10775"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYP24049.1"
                     /translation="MQFEDPRSILEAAAAKPTRGIDVNALTDRGRRMRRRRYAAVAAG
                     LAVTLAGATAAVGMLGGGRPRPGPAAAECDPQDLACLAQAWCLNPPPDEAAVFLREEA
                     SRAEVRALRDALLDRPGIESVEYVSKDEAYDEFQQLYEGEPYLWETINPGVLPASLRI
                     VVSDAAAGEALLDVTSPAIDEIRSGAGLYEQVCGEEGERPAETPAPEKRDPAAGTVTV
                     EIRWGVCRTGNARSEGGTAELTDGSKWCRFVLGVDNRATRQLRLEMEDVLLRTSDGEA
                     TVPWEDAMFGPLATRLFESPVRPGDRVLGQVTFLLPPGGVPATLEIEQTPGFAPASLL
                     VQYDCVADLREVPGRQCSFEPNSERASLRQTWARTEVSLWHCGVDPVVFGGREWVVPD
                     PPFDATNAPEGFIGRGTFKQRSSRDALFFDDGGEIIRFEAIGDWEPPVCN"
     gene            12032..13231
                     /locus_tag="VEV43_10780"
     CDS             12032..13231
                     /locus_tag="VEV43_10780"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYP24050.1"
                     /translation="MANDLPGIDSLDVEGKRVLVRCDLNVPLRDGAITDDLRIRSAVP
                     TLKALLDRGARLAVCSHLGRPKGKVVDELSLKPVGDRLAELLGTHVEVAGDVVGESAR
                     LACASNYDVVLLENLRFEPGVERNDPEMSQALARLAEAYVDDAFGAAHRAHASISGVA
                     ELLPAAAGYLLADEVTKLERLLHEPERPFVAVLGGAKVSDKLSVIGNLLERVDSICIG
                     GAMAFTLLVAQGHDVGRSLVEQDRVDEVRDVLASATERGVKVLLPSDVVAATSPDAGS
                     PCEPVPLDAIGDRLGVDIGPATAAEFSKTIEQAKTVLWNGPMGIFEVPEFAGGTRAVA
                     EAVASATRNGAYTVAGGGDSAAALKQLGMEDAVSHLSTGGGASLELLEGRELPGVAVL
                     RSKGRAL"
     gene            13228..13995
                     /gene="tpiA"
                     /locus_tag="VEV43_10785"
     CDS             13228..13995
                     /gene="tpiA"
                     /locus_tag="VEV43_10785"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYP24051.1"
                     /translation="MRTPLIAGNWKMHKTHLEAMRLVQALNHELAGHDYDKVEVVVCP
                     PFTALRTVQTLIDSDRYLIGLGAQNMHAAKEGAYTGEVTADMLKSLAVEYVILGHSER
                     REIFGETDEDVNAKVRAAIAAGLKPIMCCGETAIERENDRTERKVEGQVREGLKNVKP
                     DQARELVVAYEPIWAIGTGQTATPDDAQKTISFIRGVLRQMFPEEVADAMRILYGGSM
                     KPGNAKAIVGQPDVDGGLVGGASLDAGDFAAIVKAAV"
     gene            14014..15306
                     /locus_tag="VEV43_10790"
     CDS             14014..15306
                     /locus_tag="VEV43_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP24052.1"
                     /translation="MALKRICYVILDGLGDDPLPQLGGRTPLEAARTPLMDRLASEGR
                     NGYVTTVGEGIAPESDIAVFSILGYDPHEHHTGRGPLESVGAGLDVKDGDLAYRVNFA
                     TVEKEGDGWAILDRRVGRDLSSEEAHALAEEVQQKVRVPGASFEFRATIGHRGVLVLR
                     SDEGPLSAEVENSDPAYGRQGALGVALETFDNHVVHVTPVAGHENDPAAQRAARLTNE
                     WLRASYEVLNASETNATRTAARKLPGNFVLTRDGGDHLPDLVPFKEKFGPQMGCFVEM
                     PVELGIARLTGMGIVEAPTGIPPQEQYGKWAALALEAIEGYDGLYIHIKGPDVPAHDG
                     DYEAKIASIEEIDSLFFGPLLDELDLKKTIVAVTADHSTSCARKAHTDGPVPLLISGG
                     GVSPDGVETYGESASRKGSIGHLKGPEIMPNLVALAKG"
     gene            complement(15272..15838)
                     /locus_tag="VEV43_10795"
     CDS             complement(15272..15838)
                     /locus_tag="VEV43_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYP24053.1"
                     /translation="MYPDNLGLIASDEALAEVAGLIRDFHEASSSFDPSGYEWSDRGS
                     DGTPDGEIVCHCDLAPWNLVRRPDGSWAFIDWDLAAPGTRAWDLAWSLLTLVPLSPDF
                     TIPGADVEHRLRVFRDAYGGFAPEVVDVAVERCEREAGLIRTDPAYARLLEEGHDRIW
                     AGAGAHVARRALTWKALLSPSPGPPGSA"
     gene            16094..16498
                     /locus_tag="VEV43_10800"
     CDS             16094..16498
                     /locus_tag="VEV43_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HYP24054.1"
                     /translation="MAAARRKHTLADVLGPLESEIMDVVWDAGEVTVRDVHRALNESR
                     PIAYTTVMTTLGRLTDKGLLRRVEDQPAHRYSALVSREEYASSTVKSVVDWLVQHFPD
                     PAVAYFVDKVEKEDEEVIERLREAIDQRRDTD"
     gene            16501..>17273
                     /locus_tag="VEV43_10805"
     CDS             16501..>17273
                     /locus_tag="VEV43_10805"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYP24055.1"
                     /translation="MTSAAPAVLALETDSAWVVILAVSVVTLAAALVLRRIINRPGGV
                     LSAVLLCLPLVLPLVAALAYEHALLPEIAVLRPAGSALLDRSGDLLHLLLLQDQQTHA
                     VTPYALTGSAGPWILLAGALVTSFMLLRRVVGVLLVRRLVSRCRPLADDHLAVVGPAL
                     EDVVSLSSLPATPEVLVLPDGVQGAFACGGKRGRILISEAVIFDLDREELEGVLAHEV
                     AHLETHDVRVVFVAGLLRDLVAWNPLAHVAFRRLASDRER"
BASE COUNT         2706 a         6064 c         6069 g         2434 t
ORIGIN      
        1 gagccgcggt gtgctcgccg cggccctcgt gacgctggac gacgccgcga cgaggctcgg
       61 acgtgtcgtc tcccgcaacg actcgcacat cgaagccgag ctggacgacc tcgccgttct
      121 cctcgacgcg gtcgaggaca cgcgtgagga cctgcgcggt gcgctgcgcg ctctgccgga
      181 gatgctcgtc gcagtcgagc gagcgaactc ctacggccag tggacgaaca tccacctcgt
      241 ccacctctgc aaggacgaca ccggcgagtg cggccggagg tggatgccgt gaggtcgttc
      301 cgcgagcgca gggcctggct ggtcggcatc acctgcatcg tgctgatcgc ggccggggtg
      361 accgccgcct tctcgatcaa ccgcttccaa ggtctgcgcg gcgtgtacac gctgtcggcg
      421 gacgtggccg acgcggccgg gctgcagtcc ggcaacgagg tccgggtcgc cggggtcaag
      481 gtcgggaccg tgaagcgagt agtgctgacg ccggacgcgg cccgagtcga catggagatc
      541 caggacgacg tacgcatccc cgtcgagtcg tcgctcgaag tgaagctcaa gaccctgctc
      601 ggccagaagt tcgtcgacct gcagatgccc cgcgcctacg tcgaggcctc gtctgcggga
      661 accgatccgg acgacgcgac cgaaggctat ttcgaggccg gtgcggtgat cccgctcgac
      721 cagaccagcg tgccgttcga gatctacgag gccgcgaacg aggggacgga cgtcctcgcc
      781 cagatcgaca agcaggcgct gcgcgagctc atcgaggtgc tggacgagac cgtgggtgcg
      841 tcgaaggagg agctcggccg ggccctggac gcagtcgggc gcgccggcga cgtggtcgca
      901 cgcaactcgg ccccgctcaa gaggctgctc cgtcagtccg ccgacctcac cgggacgctc
      961 gacgccgcgg ggtccgacat cgacgggatc ctgctgcgct cggccgacgt tctggaagtc
     1021 ctcgcggaac gccgcgggac gatctcatcg cttctcgccg cgactgagga cctgtcgcgg
     1081 aacctcggcg tgctgatccg ggcgtcgaag ggctcgatcg agatcggagc cgcggacctt
     1141 aactcggtgc tgctgctcgc tcagggcgag ctcgcgacga tcgagcgcag cctggccgag
     1201 ctcgggaccg cgcaggagat gttcggccag ccgcttcggt tcggacggtt cgtcgaaggt
     1261 cacatctgct cgatcacgac cgccgacacg tgcatcccgg acgggtctcc cgaggacccg
     1321 gggctcccgg tccacgggac cgagccgggc tcgacccaca ccgtcgacag gaggcaccag
     1381 tgagggcgcg cctgatctcg ctcctcacga cgctggcgct cgtcgcggcc ggggtcgcga
     1441 cgtacacgct cacgcggacc gaggccgcca cctacaccgt gaccgcggac gtcgcacagg
     1501 caccgaacct cttcgaaggg ggccgcgtcg cagtccgggg catcgacgtg ggcgagatcg
     1561 tcgacgtaga gccgcgtggg tcggccgtgc gcgtgacgat ggagatcgac gagagcgtga
     1621 ccgtgcccgc cgacgcgcgc ctgtctatcg tcccgatcac ggtgatcgcc gaccggtacg
     1681 tgcagctcga tcccccgtac gagtcgggcc ccgtgttggc cgacggcgac cacatcgaca
     1741 tggaccgcac gacgatcccg gcggagctcg acgacgtgct gactcagctc gaggggctcc
     1801 tgtcggcgct cgagctcgag ccgggggaga ccaacggccc cctggcgcgt ctggttcgca
     1861 acctggacac cgcgctcgag ggccgggcag actcgctgca gagggggctg aagggctccg
     1921 ctgccgggct cgagaccctg gcctcgtccg agcaggacat ccggcgcctg atcgcgaacc
     1981 tggactcgtt gttcatcgtg ctcgcgcagc ggtcgagcga gatcgggctc gtcaacgagc
     2041 gcttccggag cgtcgccgag tcgctcgcgg acgaccagcg tgcgctcgag ggcacgatcg
     2101 agaacctcgc gttcctctcg cgggaggggg catcgctgat cgagacgagc ggcgacgagc
     2161 tcggcgtctc gttcggacgg ctgcgcgacg tcgcggacgc gatcctgcgc caccgggacc
     2221 agctcgagaa gggagcgcgc tggacgaacg cgatcgccca ggcactcggc gagaccgacg
     2281 cgtcgggccg cggccgatgg gcgtactcgg gacgtcaggc gccgccgggg accgagcggg
     2341 cctcgtacaa ctaccgcctc gactcccgcg acacgatctc gtgtgagcgt ctcaacgaga
     2401 tcgtgcggac ggtgctgatc ctgacgccgc aggcgccgat ggacgatctc gtcgcgaccg
     2461 tgaactcgca tctgccggac gagtacgagg acgacctcga gttcctcgtg cgccagctga
     2521 tcccggtctg caccgactgg caggcgccgg ccgtgagcgc acgcacggag cgcctcgtga
     2581 agcgcgtggc ggccgacgtc ggcgagaagc gtttcgtgaa gatgctcagc cgctggttct
     2641 gggaaggcgg gccgcgatga agctgctgcg gatcgccgcg ttgctcgcgg tcgccgtgct
     2701 cgggtcgtcg tgtgcgctca tgaccaccga tccctcgccc gaggtcgtcg cgtacttcga
     2761 agacgtcggg gacctagtcg agaagggggg cgtgcaggtc gccgacgtgg aggtcgggtc
     2821 ggtccaggag atcggcctcg tcctggagga cggccggatg ctcgcgcggg tgacgatgtc
     2881 gatcgacgag gacgagcgga tcccggccga gggcttgcgc gcggtcgtgc gccagacctc
     2941 gttgctcggc gagcagttcg tcgagctgat ccccggcccc cccggcgcgc cgtacatcgg
     3001 tggcgacgag gtcacgatcc cggtcgagga caccgaccgc atcgtcgacg tcgagacgtt
     3061 cctcggcgac ctctccgcgt tcgtgggcgg cgggggcctc gaagacctga actcgttcac
     3121 gcacgcgcag gccctgatcc tcgaagagcg cggggaccgt ctcggccgga cgatcgagga
     3181 gctcgagaag ttcacgtcgg tcctcgccgg ccgccggttc gacgtcgagg ccgcgatcga
     3241 ccacctcgcg tccgcgggtt cgacgcttgc cgccaaccgg gacacgctcg gctcgttcct
     3301 cgactccctg gaagaggcga acgcgctgct cgcggagcaa ggcgggagcc tgcgacgcct
     3361 gttcacgtcg ctgcgccgtt tcggcaccgt caactcccgc ttcctcgtcc ggcacgaaga
     3421 cgccatccac cggcagttcc gggcattgag gccgatcctc ggggggctgg cgagcgccga
     3481 aggcgagctc cgcgtcgaca tcgcgcagct gcggacgttc ctcgagctgt tcccgaagag
     3541 catcggcgga gggcccggcg gcgacggcaa gggcgactac gtccaggtcg aggccgtcct
     3601 gtgcgaggcg ctgtcggcgt gcaacacgaa gggtgagaag ggcgacgtgc cgggggaggg
     3661 atcgtgaggc tcagcccgcg tttggcgatc aacctcggcg tcgtcctcct gctgttcgtc
     3721 gtgaccgtcg gctgggtgat cacgagcgtc gtcggcccgc aggtgaccgg cgggcagttc
     3781 gaggtgaccg cggacttcgc cgagtccggc ggcgtcttca cgaaccagga ggtgacctac
     3841 cggggcgttc tcgtcgggcg cgtcggggac ctcgagctga acgaggacgg cgtcgacatc
     3901 gagctgctga tcgacggcga gtgggagggg cgcatccccg ccgactcgat cgcgcgcatc
     3961 cagagcaaga gccccgtcgg cgagcagttc gtgaacctga cgcccgggtc gtcggccggc
     4021 gagatgctag aggacggtga cgcgatcgcg cgagcgaaca cggagctgcc ggtggacttc
     4081 cagaggctgc tctcagcgtt ggaccgggtc ctcgcggacg ttccgcccga ggcgtcccgg
     4141 cgggtcatcc aggagctcgc cggcggcttg cgcggccgag gcgacgacct ggcgacgatc
     4201 ctggcctcgc tcggcacgct ggccgacacg ttcgcggacg tggcgcccga gcagcagagg
     4261 ttgctcgaca acgcgacggg agcgggggcc gagttcctgc gtacgcgcga ggcgttcgcc
     4321 gcggctctcc gcgccgcgga ccaggtcgcc gccgggatcg gcgacgagcc ggaggagctg
     4381 cgcgctctgt tcgcttctaa cgaccgcgtg gcgcgggcgg gcatccgcct gctcgcgcgc
     4441 cgcggcgacg acttcgagtc cggcatcgga gcgctgagcg acatcgtggc gttccagctc
     4501 gagcagcggg cccagatcga ggggatgctc gagtacctgc ccggcttcct gcacgcggtc
     4561 gaggacgcgt cggtgccgtg gcgctcgccg gacgggcgca agttctaccg gatccgggtc
     4621 gggctgatcc tggacaacca gccgtcgtcg tggccgtgca agtacgagct gccgaacgac
     4681 tgggagcgct tcgcgttcca gcgagacgtg cgcccggtcc ggacctcgat ggactgcatc
     4741 cccggcgagg acgaagcggc gcagcggagc gtccgggccc tggtcagatc cttgaggtcg
     4801 tgggcgaaat cgaacccgct gccggagccg gaggcggagc cgaaggccga gacgaagtcc
     4861 gccgaggccg ggctcgacga ggtcccgggc gaggcccccg taggcgaagc cgagggggcc
     4921 gagacgacct tcgaggaagc gacgcgctac ttcccggccg actggaccga gccgaacccc
     4981 tagcccggcg cgaccggcca cctggcagac ttgtgggaca aggccgccag cggagagcag
     5041 gttccatgag ggcgaagcag aacggaacca agccggtcga cgtcgtcgag gtcatgcctc
     5101 tcggcggcta tcgcgtcgcg ctcgtcttct ccgacggcca ccgggccgag cgggacctgg
     5161 gcccgtatct cgtcggcccg gtcttcgagc ccgtgcgcga ccctgactac ttccggaggg
     5221 tccgggtcga cgcacagtcg ggcacgatcg tgtggcccaa cggagcggac ctggcccccg
     5281 actcgttgcg gatgcagccg gcgccccggg actgaccgcg accaaacgcc cgggggaaca
     5341 tcacggccgg gcgagaatgg ggccgtggcc gtcaagcgga ccctccagcg ccccccggcc
     5401 gggaccatcc cggcccggcc cggcgcgtat ctcttccgcg acgcccaggg ccgcgtcatc
     5461 tacgccggca aggccaagtc gctgcgctcc cggctctcga actacttcgc ctccgacctc
     5521 cacccccgaa cgcaggccat ggtcgaggcc gccgccgacc tcgagtggat cgtcaccgag
     5581 agcgaggtcc aggccctcca cctcgagctc aacctcatca agcagcaccg cccccgctac
     5641 aacgtccgct accgggacga caagacctat ccctaccttg cgatcacgct cgacgaggag
     5701 tacccgaggg cccgcgtcct ccgcgagcgc aagagaaagg gcgtccgcta ctacggcccc
     5761 ttcgcacatg cctacgcgat ccgcgagact ctcgacctcc tgctgaggac gttccccatg
     5821 aggacgtgca gccagggcgt gttcgatcgc tgccgccgtc gcggcaagcc gtgcctcctc
     5881 ttccatatcg aacgctgcgc cggtccctgc gtcgaagccg tctccgaaga ggagcaccgc
     5941 cggatcgcgc tcgagctctg cgacttcctc gacggcaaca cgaagccggt cacccggcga
     6001 ctcgagaccg cgatgaagga ggccgcgacg acggaggagt tcgagctcgc cgcgaagtac
     6061 cgcgaccagc tcgacaacgt ccgcaaggcg atcgccaagc agcagatggt gtcgcagcaa
     6121 tcggagaacc tcgacgtgat cggcttcgcg gaggacgagc tcgaagccgc gttccaggtc
     6181 ttcttcgtgc ggggaggccg cgtgacggga cgcaaggggt tcgtcgtcga caaggtcgag
     6241 gacctcgaca cgcccgcgct cgtcgaaggc ttcctcgagc gcctctactc cgaggccgag
     6301 cccccgcccc acgtcctcgt gccggtcgag cccgaggacg cccgcctcat cgagcgatgg
     6361 ctcacggacc tgcgcggctc gaacgtcaag gtccgcgtcc cccagcgcgg cggcaagcgc
     6421 atgctgctcg agaccgtgac ggagaacgcg aagcaggcgt tcacgcagca ccgtctcaag
     6481 cgcgccagcg acttcgccgc gcggtcgcaa cagctgacgg agctccagga ggcgctcggc
     6541 atggacgacg cgccccttcg gatcgagtgc ttcgacatct cgaataccgg cccgaccgag
     6601 gccgtcggct cgatggtcgt gttcgaggac gggctcccga agaggtcgga ctatcgccgc
     6661 ttcgccatca agtgggccca gggacaggac gacttcgcga acatgggaga ggtgatccgc
     6721 cgccgcttcg cacgcttcct cgaggagcag gagaacgtcc tcgcacccga gggcaagaga
     6781 gccaagttcg cctacccccc gaacctcgtc gtgatcgacg gcgggaaagg ccagctcaac
     6841 cgggccgtcg aggtcatggg cgagctcggc gtagacggcg tgacgacggt ctcgctcgcc
     6901 aagcgcatgg aggaggtctt cgtcccgggc gaggcggaac cgattttgat cccgcggggt
     6961 tccgaagccc tctacctgct ccagcgcatc cgcgacgagg cccaccggtt cgcgctgacg
     7021 taccaccagc tacggcgcag caagaagatg acgcagtcgg cgctcgacgg gatccccggc
     7081 ctcggcgagg tgcggcgcaa gaaggtcctg aagcacttcg ggtccgtcaa acgtgtccgt
     7141 gaggcgtcac tcgaggagct gcgctccgtg cccgggctcc ctgcgacggt ggcagaggcg
     7201 atctacgaca acctccacgg gcggaaagcg tcgtgaccgc ggcccgcgac gtcgaggtca
     7261 cggtcatctc cggcctgtcg ggcgcgggga tgtccgaggc cgccaaggcc ttcgaggacc
     7321 tcgggtggtt cgtcatcgac aacctgcccc cggcattgat cggcaagatg ctgtcgctcg
     7381 cgctggggcc ggggcacgag atcaagagga tggcgctcgt catcgactcg cgcgggggca
     7441 tgttcttcag cgagatcaag gacgcgctcg cggagctgcg ccgcgacgtc gggagcttcc
     7501 ggctcgtgtt cctcgaagcc gacgacgaag ccctggtgcg gcgcttcgac gcgacgcggc
     7561 gccggcaccc gctcgcgctc caggaccacc tcacggtcgg catccagcgc gagcgaaagc
     7621 tcatgctgtc cttgcgcgac ggcgccgacc tgatcatcga cacgtcggac ctgtcggtgc
     7681 gcgacctgcg gcgcaagctc agcgcgtact tcgacgtcac ctcggcggcc gaagggctca
     7741 agaccacgat catctccttc ggctacaagt tcggcctccc gctcgacgcc gacctcgtgc
     7801 tcgacgtgag gttcctgccg aacccgcact gggtgccggg gctgcgcgag cacaccggcc
     7861 tggacgagcc cgtgcgcgac tacgtgctcg gcaaggacgt gacgggtgac ttcctcgccc
     7921 gggcgaagga cctgttcgcc gtgctgcttc ccggatacga gagcgagggc cgccactacc
     7981 tcacgatcgc ggtcgggtgc acgggcgggc ggcatcgttc ggtcgtgctc gcggaggagc
     8041 tcggggcgtt cgtccaggag aagggctacc ccgcgaaggt gatccaccgt gacgtcgaac
     8101 ggccgccggc ggtgaccgcg ccgtgaaggt gaccgggatc ggcggaggcc acggcttggc
     8161 agcgacgttg atggccgcac gtcactacgc cgacgagatc tccgcggtcg tgacggtcgc
     8221 cgacgacggg ggatcgtcgg gccagctcac gcgcgagctg ggcatacccc ctcccggcga
     8281 catccggaac tgtctggtcg cgctggcggg agacgacgag cgcgctcgcc tgtaccagca
     8341 ccgcttccag tccggagctc tcaccggcca caccgtcggc aacctcgtga tcgccgcgtt
     8401 caccgagatg accggcgact tcgcggaggc ggtcgcggca gccggacgga tgctcgggtg
     8461 cgagggccgc gtccatccgg cgacgaacga gatggtcacg ctgatggcgc aggtgcgcga
     8521 cggggtggtg cgcggtcagg tcgcggtagc gcagacgaag gacccgatcc aggcgctgtt
     8581 cctcgagccg cggtctccgc gcgctaaccg ggacgcggtg caggcgatcc tcgacgccga
     8641 ccaggtcgtc ctcgggcccg gcagcttgtt cacgagcctg atcgcgacga tcctcgttcc
     8701 cgggatccgc aaggcgctcc aggagacggc ggcccggcgc gtgttcgtgt gcaacgcgcg
     8761 gatgcagaag ggcgagaccg agggcctcga cgcggccgcg cacctcgagt ctctgctcgc
     8821 ccacgcgggc gcccggtcgg tcgacgtggt cgtggtccag tcgccggtgg tgccggtgga
     8881 cggcgtggac gtgacgtcgg tgaagtcgct cggggtccgg gtcgtcgaag cggacgtcgc
     8941 gagcgagagc ggggcgcacg acgccgaacg cctcgcggcc gtcctcgcct ctctctgagg
     9001 ccccggaggg gccgcccggt aggatggggg cctcacccat tccccaactc caggagggat
     9061 cgtcgatgac gacgcggatc gggatcaacg gcttcggccg tatcggacgg aacttcttcc
     9121 gggctctgaa ggcgaagggc tcgtcgctcg agctggtagc cgtcaacgac ctcggcgacg
     9181 ccgagacgat ggcgcacctg ctcaagtacg actcggtgca cgggcgcttc ggccgcgagg
     9241 tcaaggtctc gaacggcggc ttcacgatcg acggccagga catcaagctc ctttccgagc
     9301 gtgaccccgc ggccctcccg tggggcgatc tcggcgtcga catcgtgatc gagtcgaccg
     9361 gcttcttcac gaagcgcggc gacgcgggca agcacgtcga gggcggggcc aagaaggtca
     9421 tcatctcggc ccccgcgaag gacgaggacg tcacggtcgt catgggcgtc aaccacgaca
     9481 agtacgaccc ggcgaaccac cacatcatct cgaacgcgtc gtgcacgacg aactgcgtgg
     9541 tgccgatgac gaaggtcctc caggacaact ggggcgtcga caagggcttc atgacgacgt
     9601 gccacgcgta cacgaacgac cagaacacgc tcgacctcgc gcgcgacgac ctgcgccgcg
     9661 cccgtgccgc cgccgtgaac atcatcccga cgacgacggg ggccgcgaag gccgcgtcgc
     9721 tggcgatgcc ggagctgaag gggcgcctcg acggcctcgc gctgcgggtg ccggtgcccg
     9781 acggctcgat cacggacatc gtggcggttc tcaacgggga gccgacgaag gacgaggtga
     9841 accaggccta ccgcgaagcc gccgcgtcgg cggggatgca ggggatcctc gagtacaccg
     9901 aggacgcgat cgtctcgagt gacatcgtgg ggaactcgca cagctgcatc atcgacgggg
     9961 cctcgacgat ggtgaacgga aacatggtga aggtcctggg ctggtacgac aacgagtggg
    10021 gctactcgaa ccgtctggtc gacctgacgg agctggtggc gagctcgctg tagccgccgc
    10081 gtgcgcacgt agttgcgcaa tgcgccattg actgcgcaat cgaagggggg ccgcgcatcc
    10141 tgcggccccc cttcgtgcgt cttagaaccg tgaccgacga agaagacctg atcgctttct
    10201 gccgggccca gcacgggcgg ctcgtcgggc tcctcagcct ctactgcggc gacgccgccg
    10261 tggccgagga gctcgcccag gacgccctcg cccgggtgtg ccgtgactgg cgccggatcc
    10321 ggaagatgga ccacccgccg gcgtgggcga gccggatcgc gatcaacctc gcgaactcgt
    10381 acttcaggcg gcgatcggcc gagcggaggg cgcgcgatcg cctcgcctcg cgtccggccc
    10441 ccgacctcgg cgccctcgat accgccgccg cactgggcgt ccgccaagcg gtcgccgcgc
    10501 tgcccccgcg ccagcggacg gccctcctgc tccactactt ctgcgacctt ccgttcgccc
    10561 gcgtcgccga gctcatgggc gcacccgaac cgaccgtgaa gtcactcgca cgccgcggga
    10621 tcgcgcggct ccgctcgccc gagctcttgg gcaaccccga ggaggccccg aatgcagttt
    10681 gaggacccga ggagcatcct cgaagccgcc gcggcgaagc ccacccgggg gatcgacgtg
    10741 aacgccctga ccgacagggg ccgccggatg cgccggagac gctacgcagc ggtcgccgcg
    10801 ggcctggccg tgacgctggc cggcgcgacg gccgccgtcg gcatgttggg cgggggacgg
    10861 cctcgcccgg gcccggcggc cgccgaatgc gacccgcagg acctcgcgtg cctggcccaa
    10921 gcctggtgcc tcaacccacc gcccgacgag gcggcggtct tcctccgtga ggaagcctcc
    10981 cgagcggagg tgcgagcgct gcgtgacgcg ctcctcgacc ggcccggcat cgagagcgtc
    11041 gaatacgtct cgaaggacga ggcctacgac gagttccagc agctctacga gggcgagccg
    11101 tatctgtggg agacgatcaa ccccggtgtt ctgcccgcga gcctcaggat cgtcgtgtcg
    11161 gacgccgccg ccggagaagc gctccttgac gtcacctcgc cggcgatcga cgagatcagg
    11221 agcggcgccg gcctctacga gcaggtgtgc ggggaggagg gggagcgccc ggcggagacc
    11281 ccggcccccg agaaacgcga ccctgcggcc ggcacggtca ccgtcgagat ccgctggggc
    11341 gtgtgcagga ccggcaacgc ccggtccgag ggggggaccg ccgagctcac cgacggcagc
    11401 aagtggtgca ggttcgtcct cggcgtcgac aaccgtgcaa ccaggcagct gcgcctcgag
    11461 atggaagacg tcctgctgcg gacctccgac ggcgaggcga ccgtgccgtg ggaggacgcg
    11521 atgttcgggc ccctggcgac gaggctgttc gagtctcccg tccgccccgg cgatcgagtc
    11581 ctcggccagg tcaccttcct cctgcccccg ggcggcgtcc cggcgacgct cgagatcgag
    11641 cagacccccg gcttcgcccc ggcgtccctc ctcgtgcaat acgactgcgt agcggacctc
    11701 cgtgaggtcc cgggccggca gtgctcgttc gaaccgaact ccgaacgggc gtcgctgcgg
    11761 cagacgtggg cgaggaccga ggtctcgctg tggcactgcg gggtcgaccc ggtcgtcttc
    11821 gggggccgcg agtgggtcgt cccggatccc cccttcgacg cgacgaacgc ccccgagggc
    11881 ttcatcgggc gcggcacgtt caagcagagg tcgtcgaggg acgctctctt cttcgacgac
    11941 gggggcgaga tcatccgctt cgaagcgatc ggggactggg aaccaccggt ctgcaactga
    12001 cgcctcgcga ccttctacac tcgcccccgt catggccaac gacctgccgg ggatcgactc
    12061 gctcgacgtc gagggcaagc gcgtcctcgt ccgctgcgac ctgaacgtcc ccctgcgcga
    12121 cggcgccatc accgacgacc tccgcatccg gtcggccgtc ccgacactga aggccctgct
    12181 cgaccgcggc gcccggctcg ccgtctgctc gcacctcggc cgccccaagg gcaaggtcgt
    12241 cgacgagctg agcctgaagc ccgtggggga ccgcctcgcc gagctcctcg gcacccacgt
    12301 cgaggtcgcc ggagacgtcg tcggcgagag cgcacggctc gcgtgcgcga gcaactacga
    12361 cgtcgtcctg cttgagaacc tgcgcttcga gccgggcgtg gagcgcaacg acccggagat
    12421 gagccaggcc ctcgcgcgtc tagcggaggc ctacgtcgac gacgcgttcg gagcggcgca
    12481 ccgtgcgcac gcctcgatct cgggagtcgc cgagctcctc cccgcggccg ccggctacct
    12541 cctcgccgac gaggtgacga agctcgaacg gctcctccac gagcccgagc gcccgttcgt
    12601 ggcggtgctc ggcggcgcga aggtctccga caagctcagc gtcatcggga acctcctcga
    12661 acgcgtcgac tccatctgca tcggaggcgc gatggcgttc acgctgctgg tcgcgcaggg
    12721 gcacgacgtc ggccgatcgc tcgtcgagca ggaccgcgtc gacgaggtgc gcgacgtcct
    12781 cgcgagcgcg accgagcggg gcgtgaaggt cctgctcccg agcgacgtcg tggcggccac
    12841 ctcgccggac gcggggtcgc cctgcgagcc cgttccgctc gacgccatcg gcgaccggct
    12901 cggcgtcgac atcggcccgg cgaccgcagc cgaattctcg aagaccatcg agcaagcgaa
    12961 gaccgtgttg tggaacggcc cgatgggcat cttcgaggtc ccggagttcg ccggcggtac
    13021 gcgggcggtc gcggaggccg tcgcgagcgc gacccgcaac ggcgcctaca cggtcgccgg
    13081 aggaggcgac tcggccgcgg ccctcaagca gctcgggatg gaggacgcgg tctcgcacct
    13141 gtctaccggc ggaggggcgt ctctcgagct cctggagggc cgggagctac cgggggtcgc
    13201 ggtattgaga tcgaaaggac gcgctctatg aggactccgc tgatcgcggg caactggaag
    13261 atgcacaaga cgcacctcga ggcgatgcgc ctcgtccagg cgctgaacca cgagctcgcc
    13321 ggccacgact acgacaaggt cgaagtcgtc gtgtgcccgc cgttcaccgc gctgcggacc
    13381 gtgcagacac tcatcgactc cgaccgctac ctgatcgggc tgggcgcgca gaacatgcac
    13441 gccgcgaagg agggcgcgta cacgggcgag gtcacggccg acatgctcaa gtcgctcgcc
    13501 gtcgagtacg tgatcctcgg ccactcggag cgccgcgaga tcttcggcga gaccgacgag
    13561 gacgtgaacg cgaaggtgcg ggccgcgatc gccgcggggc tcaagcccat catgtgctgc
    13621 ggcgagaccg cgatcgagcg cgagaacgac cgtacggagc gcaaggtcga gggccaggtc
    13681 cgcgagggcc tcaagaacgt caagcccgac caggcgcgcg agctcgtcgt cgcgtacgag
    13741 ccgatctggg cgataggcac gggacagacc gcgacgcccg acgacgcgca gaagacgatc
    13801 tcgttcatcc gcggcgtcct gcgccagatg ttcccggagg aggtcgccga cgcgatgcgg
    13861 atcctctacg gcgggagcat gaagcccggc aacgcgaagg cgatcgtcgg ccagcccgac
    13921 gtcgacgggg gcctcgtcgg cggggcgtcc ctcgacgcgg gtgacttcgc cgcgatcgtc
    13981 aaagccgccg tctagacaac cgcaaggaga gaaatggccc tcaagcgcat ctgctacgtg
    14041 atcctcgacg gtctcgggga cgacccgttg ccgcagctcg gcggacgcac gccgctcgaa
    14101 gccgcgcgca cgccgttgat ggaccggctc gcgagcgagg gccgcaacgg ctacgtcacg
    14161 acggtcggcg agggcatcgc tcccgagtcc gacatcgcgg tgttctcgat cctcggatac
    14221 gacccgcacg agcaccacac cggccgcggc cccctcgagt ccgtgggcgc gggcctggac
    14281 gtcaaggacg gcgacctcgc ctaccgcgtg aacttcgcaa cggtcgagaa ggagggcgac
    14341 ggctgggcga tcctcgaccg gcgcgtcggc cgtgacctct cttcggagga ggcccacgcg
    14401 ctcgccgagg aggtccagca gaaggtccgc gtccccggcg cgtcgttcga gttccgcgcg
    14461 acgatcggcc accgcggcgt gctcgtgctg cgatcggacg aaggcccgct ctcggccgag
    14521 gtcgagaact ccgaccccgc gtacggtcgc cagggcgcgc tcggcgtcgc gctcgagacg
    14581 ttcgacaacc acgtcgtgca cgtgacgccc gtggcgggcc acgagaacga tcccgccgcg
    14641 caacgagccg cacgcctgac gaacgagtgg ctgcgcgcaa gctacgaggt cctgaacgcg
    14701 agcgagacca acgcgacgcg cacggcggcg cgcaagctcc cgggcaactt cgtgctcacc
    14761 cgggacggtg gcgaccacct cccggatctc gtcccgttca aggagaagtt cggcccccag
    14821 atgggctgct tcgtcgagat gccggtcgag ctcgggatcg cgcggctcac cggcatgggc
    14881 atagtcgagg cgccgaccgg gatcccgccg caagagcagt acggcaagtg ggccgcgctc
    14941 gcgctggagg cgatcgaggg ctacgacggc ctctacatcc acatcaaggg gcccgacgtc
    15001 ccggcgcacg acggcgacta cgaggcgaag atcgcgagca tcgaggagat cgactctctg
    15061 ttcttcgggc cgctgctcga cgagctcgac ctgaagaaga cgatcgtcgc ggtcacagcc
    15121 gaccactcga cgagctgcgc gcgcaaggcc cacaccgacg gccccgtgcc gctgctgatc
    15181 tcgggcgggg gagtgagccc cgacggcgtc gagacctacg gcgagagcgc gtcacgcaag
    15241 gggagcatcg gccacctcaa ggggccggag atcatgccga acctggtggc cctggcgaag
    15301 ggctgagcaa cgccttccac gtcagtgcgc ggcgcgccac gtgcgcgccg gcgccggccc
    15361 atatccggtc gtgcccttcc tcgaggagcc gggcgtacgc ggggtccgtg cggatgagcc
    15421 cggcctcgcg ttcgcaccgc tcgacggcga cgtcgacgac ctcgggcgcg aaccccccgt
    15481 aggcgtcccg gaagacccgg aggcggtgct cgacgtcggc gcccgggatc gtgaagtccg
    15541 gcgacagcgg gaccagcgtc agaagcgacc acgcgaggtc ccaggcccgc gtcccggggg
    15601 ccgcgaggtc ccagtcgatg aacgcccagc tcccgtccgg acgccggacg aggttccacg
    15661 gcgcgaggtc gcagtggcac acgatctcgc cgtccggcgt cccgtcggac ccgcggtcgg
    15721 accattcgta gccgctcggg tcgaacgaag aagacgcctc gtggaagtcg cggatcagcc
    15781 ccgccacctc cgccagcgcc tcgtcggacg cgatcaggcc gaggttgtcc ggatacacga
    15841 cgtccccctc gacgaacgtc aggacctcga cggcccccct cgtcggtccc gagcacccgg
    15901 ggggcgccgt cgaaccccgt cgcctccagg tggcgcagga acgcgtggac cgccggcgtc
    15961 cacggcccgg tcgggcggcg gacggtgtcg cccaccctca cgatcgactc gtgggtgttc
    16021 ccgccgggca gcacctcttc gtccacgctg ctctactatt gcagtaggtc aacgggaatg
    16081 gggtgtcgcc tagatggctg ctgctcgcag gaagcacacg ctcgccgacg tcctcggccc
    16141 cctcgagtcc gagatcatgg acgtcgtatg ggacgcgggt gaggtcaccg tccgcgacgt
    16201 ccaccgcgcg ctcaacgaga gccggccgat cgcctacacg accgtgatga cgacgctcgg
    16261 acgcctcacc gacaagggtc tcctccggcg tgtggaggac cagcccgccc accgttactc
    16321 ggccctcgtg tcgcgcgagg agtacgccag ctccaccgtg aagtcggtcg tcgactggct
    16381 cgtccagcac ttccccgacc ccgccgtcgc gtacttcgtc gacaaggtcg agaaggagga
    16441 cgaagaggtg atcgagaggc tccgagaggc catcgaccag cggagagaca cggactgacc
    16501 gtgacgagcg cggcccccgc ggtcctcgcg ctggagacgg actcggcctg ggtcgtgatc
    16561 ctggccgtct ccgtggtgac gctcgccgcg gccctcgtgc tgcggcggat catcaaccgg
    16621 cccggaggtg tcctctccgc cgtcctgctg tgccttcctc tcgtcctgcc gctcgtcgcg
    16681 gccctcgcct acgagcacgc gctgctcccc gagatcgcgg tcctccggcc ggccggctcg
    16741 gccctcctgg accgttcggg cgacctcctc catctgctcc ttctgcagga ccagcagacg
    16801 catgcagtca cgccctacgc gctgacgggc tcggccgggc cgtggatcct gctggccggc
    16861 gccctcgtca cgtcgttcat gctcctgcgc cgcgtcgtcg gcgtcctgct cgtgcgccgg
    16921 ctcgtctcgc gctgccgccc gctcgccgac gatcacctcg cggtcgtcgg cccggccctc
    16981 gaagacgtcg tgagcctgtc gtcgctcccg gcgactcccg aggtcctcgt gctccccgac
    17041 ggggtccagg gggcgttcgc ctgcggcggt aagcgcgggc gcatcctgat ctccgaagcc
    17101 gtgatcttcg acctcgaccg cgaagagctc gagggagtgc tcgcccacga ggtcgcacac
    17161 ctcgagaccc acgacgtaag ggtggtgttc gtcgccggcc tcctgcgcga cctcgtcgcg
    17221 tggaacccgc tggcgcacgt cgccttccgg cggctcgcgt ccgaccgcga gcg
//
LOCUS       DASQDO010000383         7424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1142199, whole genome shotgun sequence.
ACCESSION   DASQDO010000383 DASQDO010000000
VERSION     DASQDO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142199"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VEV43_10810"
     CDS             <1..444
                     /locus_tag="VEV43_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24056.1"
                     /translation="LCAITGSFLDWAVITPRQVAPDVDFGEDTDEIEPGQGEPFKGVD
                     DRDGRLTLVGGVALIVAAIALVVRKKSGVAWVAFWISLAIGGIAFADFRTVSANDDAA
                     PRGILLRHEVGAEASAGPGLAVVAAAGMLGVLSSVGGVAATPRET"
     gene            complement(449..3163)
                     /locus_tag="VEV43_10815"
     CDS             complement(449..3163)
                     /locus_tag="VEV43_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HYP24057.1"
                     /translation="MARVRRSIRPGQVLELPHIQEAIARGFIALDGDRIRYSLHKDRA
                     YAWKDPEEWVRAAIVAYLIVEKDYSPARMELEVPIPRRVPGDYADVVVFRDDRCKDPY
                     IVVEAKAAGISETARAQGIEQAVGGAHSLRAPFALYYDHVSSLLLDTSGAYPAGERVA
                     NRRGGHQSVPRQYSEDEPSFRFVAAGPDDITRADARRLENKVRRTHSIIWSGGKRDPL
                     TSFDEWSKLLFAKVHDERTTPNGVPRRFQVGSGETATAVANRIHHLFADAMKVDKSIF
                     RSEARLLLSDDKIRDIVEVLQDVSILNTEADTIGAAFERFFGSVFRGELGQYFTMREI
                     ARFVVGAVGVDHRHFVIDPTAGSGGFLLEALLQGWHSIENDFSGQSEIERKRIEFAMQ
                     QVFGIEIHETLARILKINLLLHHDGHTNIEGDRSCLDVDFGNQRLRTSWRGGFDRVIG
                     NPPFGDDVRAGDGDLLGTNELSAFAVARDRAQVPSEHVILERGIDMLRPGGRLAFVIP
                     DGLLNNQGEPSNCPRVRRLLARSGVIEAIVSLPHHAFRKSGAQNKTSILMFRTFFDSE
                     RAEWDAAYGAAEAAGADEDAAVLEGLRALDYDVFLAEATHVGYTPTGQKSDSNDLYVS
                     DERGYVADGQEGSILGELWRFRAAPGTYDGSRSPDCMSLSVVDMWKAHPSHRLDPKYF
                     LFKREERSPAPEGWITARVGEVMRRRRERQRPEDDPDREVVVLTVPQSGDLRQRPAGK
                     GRNPPEWRGMYFEGMPTKWYAVRSGDVVFSGIDLWKGCIAVVPDEFDGALVSGEYPVY
                     EVVDERLDADFLSILLRSRHYRRAFRAITTGHSNRLRTQPEDFEDLEISFPVSKDEQR
                     RLIAEAVRARRALREADVSLREAMLKFSDVIDGRGDEEYPVEEEDAPP"
     gene            complement(3244..4086)
                     /locus_tag="VEV43_10820"
     CDS             complement(3244..4086)
                     /locus_tag="VEV43_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYP24058.1"
                     /translation="MRIRRYRGRHLKPRPRGKAPAAVGAATVVWFAGGSAQAGTHVVR
                     AGETLSSIAARSRTTVDALVKANDLTNPDFVVAGQHLRVRTPATRHRVRPGETLSSIA
                     ARFGTSVDRIARANHLSNPNLIVPGQSLRVPGGVPVATTVSTPATPPAVPVAVVEDSL
                     ERQSAAHGVRTSLVKAVAMKESAWRQDAVSSAGALGVMQVMPDTADFVNEVLDPGPEL
                     NVRDADDNVHLGVMYLDHMLERMPSERDALAAYYSGPGNVGPRLDRGQRDYVADVKDL
                     ERDF"
     gene            4240..4437
                     /locus_tag="VEV43_10825"
     CDS             4240..4437
                     /locus_tag="VEV43_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24059.1"
                     /translation="MRKSLSLLVAAAAVAGSIATAAPAHAWTCAINDEYVPEEVGDAA
                     CFVVLTVAGTVCKVAKPACFA"
     gene            complement(4488..5738)
                     /locus_tag="VEV43_10830"
     CDS             complement(4488..5738)
                     /locus_tag="VEV43_10830"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HYP24060.1"
                     /translation="MVEVHRTVFLLALAATLTIAPGAIAQTETPSPVPSPTQVPPDTT
                     PPAITIGTRDARVVLGTLDKLAAIRASFSEPSSVTIRVTSRSGRRIVQVPIAERAVSR
                     VAAKWAGGGVRGRLVRPGRYSFVVRARDAAGNGSVKRVPVRAVASSYAGAAPLHYTYV
                     LEGRGVPRSAMRDFATVAAETLADMKGWSLRHNVSYRRVASGGNFRLILASPGSVAAA
                     SPACSSYWSCRVGSQVLINLDRWRYATPSWDQPRREYRSFVINHEVGHWLGLGHAGCG
                     GTGRPAPVMMQQSKGLYGCRHNAWPLESERARVAALRGGTAWPLMPLASPCTIVGTGR
                     RDVLRGTGRPDVVCGLGGDDVLHGLGGDDVLVGGGGRDELRGGPGSDRARGPHDRCTS
                     CGVHRRQLAEPVAPPVRRVRVDVE"
     gene            5818..6420
                     /locus_tag="VEV43_10835"
     CDS             5818..6420
                     /locus_tag="VEV43_10835"
                     /inference="COORDINATES: protein motif:HMM:NF042714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pYEATS domain-containing protein"
                     /protein_id="HYP24061.1"
                     /translation="MADIVPLLQTLSWVLLIAGLVWVFRDRLNPLFDAVAERLRYSRL
                     KGPGGFEVGEDLRTLPAVEPGGRVAETSTFSETATVTATDDLVGQRKALYKDSRRVFL
                     AHVLEPTKITGQKFEIFIYLVRHRGGDLEDVARAEFYLGKHWGDKVFEVEQSSGEPLG
                     FRTAAYGPFLCVCRVTFSDGGTTLISRYIDFEMGELLRGQ"
     gene            complement(6476..>7424)
                     /gene="nadA"
                     /locus_tag="VEV43_10840"
     CDS             complement(6476..>7424)
                     /gene="nadA"
                     /locus_tag="VEV43_10840"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HYP24062.1"
                     /translation="THTDQGWADEVRRLAAEQDAVILAHNYQVPAVQDVAHFVGDSLE
                     LSRIAAEVDESTIVFCGVHFMAETAKILAPEKRVLIPDAGAGCSLAASITADQLRAWK
                     AEHPGAVVVMYVNTTADVKAETDICCTSANAVKVVQSIPEDKEILFGPDMFLGAHVER
                     ESGRKMHVWMGECHVHAGIQPSDIQQMMASHPDAELHVHPECGCSSACMYMLSMGDLP
                     KDRTFVLGTGGMVRRAHESTASEFIVATETGMLHRLRKENPKGNYIPANEGAVCGYMK
                     MITASKLLRCLQTGVDEVTVPPDIAEKARRPIERMISII"
BASE COUNT         1151 a         2554 c         2503 g         1216 t
ORIGIN      
        1 ctctgtgcga tcacgggctc gttcctcgac tgggccgtga tcaccccgcg gcaagtcgcc
       61 ccggacgtcg acttcggcga ggacaccgac gagatcgagc ccggtcaggg cgagccgttc
      121 aagggcgtgg acgaccggga cggccgcctc acgctggtgg gcggcgtcgc gttgatagtg
      181 gcggcgatcg ccctcgtcgt gcgcaagaag agcggggtcg catgggtcgc gttctggatc
      241 tcgctcgcga tcggaggcat cgcgttcgca gacttccgga cggtgtcggc gaacgacgac
      301 gcggctccgc gcgggatcct gttgcgccac gaggtggggg ccgaggcgag tgcggggccg
      361 ggcctcgcgg tcgtcgcggc cgcggggatg ctcggtgtgc tctcgtcggt cgggggcgtc
      421 gccgctaccc cgcgcgagac ctagacgcct aggggggcgc gtcctcctcc tccaccggat
      481 attcctcgtc accccgtccg tcgatcacgt cgctgaactt caacatggcc tccctcaacg
      541 agacatcggc ttcacgaagc gcccgtctcg cgcgcaccgc ctcggcgatc agacggcgtt
      601 gttcgtcctt cgacaccggg aagctgatct ccaagtcctc gaagtcctcg ggctgtgttc
      661 gcaggcggtt gctgtggccg gtcgtgatag cgcggaacgc acgccggtag tgccggctcc
      721 ggagcaggat ggacaggaag tccgcgtcca gcctctcgtc gacgacctcg tagaccgggt
      781 actcgccgct gacgagcgcg ccgtcgaact cgtccgggac gaccgcgatg caacctttcc
      841 agaggtctat ccccgagaag acgacgtcac cgctgcgcac ggcgtaccac ttcgtcggca
      901 tgccctcgaa atacatcccg cgccactcgg gcgggttgcg acccttgccg gccggacgct
      961 gtcggaggtc gccgctttga gggaccgtga gcaccaccac ctcgcgatcc gggtcgtcct
     1021 ccggccgctg ccgttccctg cgccggcgca tgacctcgcc gacccgcgcg gtgatccaac
     1081 cctcgggcgc cggcgaccgt tcctcgcgct tgaacaggaa gtacttgggg tcgagccgat
     1141 ggctcgggtg cgccttccac atgtcgacga cgctcagcga catgcagtcg ggggaccgac
     1201 tgccgtcgta ggtgcccggg gcggcacgga accgccacag ctcaccgagg atcgacccct
     1261 cctggccgtc cgcgacgtac ccccgttcgt cgctcacata cagatcgttc gagtcggact
     1321 tctggccggt cggcgtatac ccgacgtgcg tcgcctccgc gaggaacacg tcgtagtcga
     1381 gcgcgcgcag cccctccagg acggcggcgt cctcgtccgc gccggcggcc tccgcggcac
     1441 cgtacgccgc gtcccactcc gcgcgctccg aatcgaagaa cgtcctgaac atcaggatcg
     1501 acgtcttgtt ctgagctccc gacttgcgga acgcgtgatg aggaagagac acgatcgcct
     1561 cgatgacgcc actccgggcg aggagcctcc ggacccgcgg gcagttcgac ggctcgccct
     1621 gattgttgag caagccgtcc gggatgacga acgccagccg gccgccggga cgcagcatgt
     1681 cgatgccgcg ttcgaggatc acgtgctcgg acgggacctg ggcccgatcg cgggcgactg
     1741 cgaacgcgga gagctcgttc gtcccgagaa gatcgccgtc accggccctg acgtcgtcgc
     1801 cgaacggtgg gttcccgatc acccggtcga acccgcccct ccacgacgtc cgcagacgtt
     1861 ggttgccgaa gtcgacgtcg aggcagctcc tgtccccctc gatgttcgtg tgcccgtcgt
     1921 ggtgcaggag gaggttgatc ttcaggatgc gcgccagcgt ttcgtggatc tcgatcccga
     1981 agacctgctg catcgcgaac tcgatcctct tgcgctcgat ctcggactgg ccgctgaagt
     2041 cgttctcgat ggagtgccat ccctggagga gggcctccag caggaacccg ccgctccccg
     2101 ccgtcgggtc gatcacgaag tggcggtggt caactcccac ggcgcccacg acgaaccggg
     2161 ctatctcgcg catcgtgaag tactgaccga gctcgccgcg gaagaccgac ccgaagaagc
     2221 gctcgaacgc cgcgccgatc gtgtccgcct ccgtattgag gatgctgacg tcctgcagga
     2281 cctcgacgat gtcgcggatc ttgtcgtcgg acagcaacaa gcgggcttcc gaccggaaga
     2341 tgctcttgtc caccttcatt gcgtcggcga acaagtggtg gatgcgattc gcaaccgccg
     2401 tcgcggtttc gccggatccc acctggaacc tgcgcgggac cccgttgggc gtcgtccgtt
     2461 cgtcgtggac cttcgcgaag agcagcttgc tccattcgtc gaacgacgtg agcggatcgc
     2521 gcttaccacc cgaccagatg atcgagtggg tccggcgcac cttgttctcc agccggcggg
     2581 catcggcccg ggtgatgtcg tcggggcccg ctgctacgaa gcggaacgac ggctcgtcct
     2641 cggaatactg tcgcggaacg ctctgatggc cgccgcgccg gtttgcaaca cgctctccgg
     2701 cggggtacgc gccgctcgtg tcgaggagca acgacgagac gtggtcgtaa tagagcgcga
     2761 agggcgcgcg gaggctgtgc gccccgccta ccgcctgttc gatgccttgc gctcgcgcgg
     2821 tctccgagat gcctgccgcc ttcgcctcga ccacgatgta cgggtccttg catctgtcgt
     2881 cccggaagac gacgacgtct gcgtagtctc cggggacgcg ccgcgggatc ggcacttcga
     2941 gctccatccg cgccggggag tagtccttct cgacgatcag gtacgcgacg atcgcggctc
     3001 tgacccattc ctcggggtcc ttccatgcgt acgcccggtc cttgtggagc gagtagcgga
     3061 tccggtcgcc gtcgagggcg atgaacccgc gcgcgatcgc ttcctgtatg tgaggaagct
     3121 ccaggacctg tcccggccgg atggaccggc gaacccgcgc cacgatcgcc cgggttcact
     3181 gcgcgcgatg ggcaaacctg ctatccgaaa gtgattcgtg cgattaagcg atgagacaac
     3241 gacctagaag tcgcgttcca gatccttcac gtctgcgacg tagtcgcgct gcccgcggtc
     3301 gagccgcggc ccgacgttgc ccggccccga gtagtacgcg gccagcgcgt ccctctcgga
     3361 cggcatccgt tcgagcatgt ggtcgaggta catcacgccg aggtgcacgt tgtcgtccgc
     3421 gtcgcggacg ttgagctccg gccccgggtc gaggacctcg ttgacgaagt cggccgtgtc
     3481 gggcatcacc tgcatgacgc cgagcgcacc ggcggacgac accgcgtcct gccgccacgc
     3541 gctctccttc atcgcgacgg ccttcacgag cgaggtccgg accccgtgcg ccgccgactg
     3601 gcgttcgagg gaatcctcga cgacggcgac ggggaccgcc ggcggagtcg ccggggtgga
     3661 gaccgtggtg gcgaccggaa cgccgccggg cacgcggagg ctctggccgg gcacgatgag
     3721 gttcgggttc gacaggtggt tcgctcgcgc gatccggtcc acgctggtcc cgaacctcgc
     3781 tgcgatgctc gacagcgtct cgcccggccg gacacgatgc cgggtcgccg gcgtccggac
     3841 gcgcagatgc tggccggcta cgacgaagtc cgggttcgtc aggtcgttcg ccttcactag
     3901 ggcgtcgacc gtcgtgcggg agcgcgccgc gatgctcgac agagtctcgc cggcgcgaac
     3961 gacgtgggtc ccggcctggg cggacccacc cgcgaaccag acgacggtcg cggcgccgac
     4021 ggcggcggga gccttccccc ggggccgggg cttcaagtgc ctgccccggt agcgcctgat
     4081 cctcatctct cacctcacgg cccaatagag aacagacctc acagaatgca catgcttcac
     4141 acataggtca aatgggtgat tccagccagg cgagcaggac ggcgggggtc tgcacccgaa
     4201 caatcagccg tcgcactccg ctagaggaag gacgagaacg tgcggaaatc gctgagcctg
     4261 ctggtcgccg ccgccgccgt tgccgggagc atcgccaccg cggcccccgc gcacgcatgg
     4321 acgtgtgcaa tcaacgacga gtacgtcccg gaggaggtag gcgacgccgc gtgcttcgtc
     4381 gtgctcacgg tggccgggac cgtctgcaag gtcgccaagc ccgcctgctt cgcctgatcc
     4441 ggccgtagtc gcgaaaggcc ccccgctgag cggggggtct ttcgcatcta ctccacgtcg
     4501 acccgcacgc gtcgaacagg cggagccacc ggctcggcga gctgccgccg gtgcacgccg
     4561 cagctcgtgc agcggtcgtg cggcccccgg gctcggtcgg agccgggccc cccgcgtagc
     4621 tcgtcccgtc cgccacctcc gacgaggacg tcgtccccgc cgagaccgtg caggacgtcg
     4681 tcgcccccga gcccacacac gacgtcgggg cggccggtac ccctcaacac gtcgcgtcgg
     4741 ccggtcccga cgatcgtgca cggcgacgcg agcggcatca aaggccacgc cgtcccgccg
     4801 cgcagagccg cgacccgggc tcgttcgctc tccaacggcc acgcgttgtg ccggcagccg
     4861 tacagaccct tggactgctg catcatcacg ggggccgggc gacccgttcc gccgcaaccg
     4921 gcgtgcccca gcccgagcca gtgcccgacc tcgtggttga tcacgaagct gcggtactcg
     4981 cgcctcggct ggtcccacga cggcgtcgcg tagcgccagc ggtcgaggtt gatcagcacc
     5041 tgggagccga cgcgacagct ccagtacgag ctgcacgccg ggcttgctgc ggcgaccgac
     5101 cccggcgagg cgaggatcag gcggaagttc ccgccggagg cgacgcggcg atagctcacg
     5161 ttgtggcgga gcgaccaccc cttcatgtcg gccagggtct cggccgccac cgtcgcgaaa
     5221 tcgcgcatcg ccgaccgcgg cacgccccgg ccctcgagca cgtacgtgta gtggaggggg
     5281 gccgcgccgg cgtacgagct ggcgacggcg cgcacgggga cccgcttgac cgagccgttg
     5341 cccgccgcgt cccgagcccg gacgacgaac gagtacctcc ccggccgcac gagccggcca
     5401 cggacaccgc cgcccgccca cttggcggcg acacgtgaca cggcacgctc tgcgatcggt
     5461 acctgcacga tgcgacgacc ggagcgcgag gtcacccgga ttgtgacgga agagggctcc
     5521 gagaacgacg cgcggatcgc ggcgagcttg tcgagcgtgc cgaggacgac gcgggcgtca
     5581 cgtgtcccga tcgtgatggc cggtggggtc gtgtcggggg gcacctgcgt gggagacggc
     5641 accggtgacg gagtctcggt ctgggcgatc gccccgggcg cgattgtcag cgtcgccgcc
     5701 agtgccagaa ggaacacggt ccggtgcacc tcaaccacgg tggcagcgtg ggggaggcgg
     5761 cgcaaggggg ataggtgggg ttcaatcgaa acctgcccaa caaaccgggg aggaatcgtg
     5821 gcggacatcg ttcccttgtt gcagacgttg tcgtgggttc tgctcatcgc gggactggta
     5881 tgggtcttcc gcgaccgatt gaatccgctg ttcgatgcag tagcagaacg tctccgctat
     5941 agccgcttga agggcccagg aggattcgag gtcggtgagg acttacgtac gctaccggcg
     6001 gtggaacccg gagggcgcgt cgccgagacg tctacttttt cagagacagc gaccgtcact
     6061 gccaccgatg acctcgtggg tcagaggaaa gccctctaca aagacagcag gagagtcttt
     6121 ctcgcccatg tcttggaacc gacgaagatc actggacaga agttcgagat cttcatctac
     6181 ctcgtccgtc accgaggggg tgacctggag gacgtggcgc gcgcagagtt ctacttgggc
     6241 aagcactggg gcgacaaggt gtttgaggtc gaacaatcct cgggggagcc tctaggcttc
     6301 agaaccgcgg cgtatgggcc gttcctctgt gtctgtcgtg tgaccttctc cgacggcggc
     6361 accacgctca tttcccgata tatcgacttc gagatgggcg aactgttgcg cgggcagtga
     6421 tcccgcacct ctcccgcagg cagattcgac gggccacatc cccgcgtcgc gaccgctaga
     6481 tgatcgagat cattcgttcg atcgggcggc gggctttctc ggctatgtcc ggcggcaccg
     6541 tcacctcgtc cacccccgtc tgcaggcagc gcagcagctt cgacgccgtg atcatcttca
     6601 tgtagccgca caccgcgccc tcgttcgcgg ggatgtagtt gcccttcggg ttctccttgc
     6661 ggagccggtg gagcattccc gtctcggtcg cgacgatgaa ctccgacgcg gtcgactcgt
     6721 gcgcgcggcg caccatgccc ccggtcccca gcacgaacgt gcggtccttc ggcaggtcac
     6781 ccatcgacag catgtacatg cacgccgacg agcatccgca ctcggggtgg acgtggagct
     6841 cggcgtcggg atgcgacgcc atcatctgct ggatgtcgga cggctggatg ccggcgtgca
     6901 cgtggcactc gcccatccag acgtgcatct tgcggccgct ctctcgctcg acgtgcgcgc
     6961 cgaggaacat gtcggggccg aacaggatct ccttgtcctc ggggatcgac tggaccacct
     7021 tcaccgcgtt cgccgaggtg cagcagatgt cggtctcggc cttcacgtcg gcggtcgtgt
     7081 tcacgtacat cacgacgacg gcgccggggt gctcggcctt ccacgcgcgc agctggtcgg
     7141 ctgtgatcga cgcggcgagc gagcagcccg cgcccgcgtc cgggatcagc acgcgcttct
     7201 cgggggcgag gatcttggcg gtctcggcca tgaagtgcac gccgcagaac acgatcgtgg
     7261 actcgtcgac ctcggccgcg atccgcgaca gctcgagcga gtcgcccacg aagtgcgcga
     7321 cgtcctgcac ggctgggacc tgatagttgt gggcgaggat gaccgcgtcc tgctcggccg
     7381 ccagccggcg aacctcgtcg gcccacccct ggtcggtgtg cgtc
//
LOCUS       DASQDO010000384         7887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1203352, whole genome shotgun sequence.
ACCESSION   DASQDO010000384 DASQDO010000000
VERSION     DASQDO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203352"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VEV43_10845"
     CDS             <1..937
                     /locus_tag="VEV43_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HYP24063.1"
                     /translation="QEAWEETVRMLEVYREVATDVLAIPVLAGRKSESERFPGADETL
                     PIEGLMRDRKALQCGTSHFLGQNFSKAYDVQFLNKEGEQEYAWGTSWGFSTRMIGGTI
                     MAHGDDRGLRLPPAIAPVQVVVVPIYRNDEEQSSVLGAAAKIRDALAGDGIKVRVDDR
                     DQHRPGFKFSEWELKGVPIRIEIGPRDLAADQVTLADRITGDKEQVPIDQTIAGMRGR
                     LDASQAALLADATAFRDANTHEATTFDQLKEGVESEGGFWIGPWCGDAACEEKVTAET
                     KATIRVLPLEREDPGAACLVCGSSGNERATWAKAY"
     gene            991..3618
                     /locus_tag="VEV43_10850"
     CDS             991..3618
                     /locus_tag="VEV43_10850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYP24064.1"
                     /translation="MARPWRLPTADVAFDEAQRKIEVLESQLEASLEGSLVVWMDGSI
                     VSFNRRFVEMWSLPPDALETESDQDITQTIFDQLVDPRGFEERVLDLYRHPERTPRDE
                     LRLKDGRVFEAYGTPLQDEGGRYHGWAWFFRDVSDRKRTEQRLAAQYEVVKVLAEAGS
                     LAEAAPRLLRAVADGFGWACGTLWRVDHDTEMLRCVDVYTQTGTDTQSFVEVSRALLV
                     PRGGGLPGIVWESGKPEWVTNVHENPDFVRSSAARQAGLHAAILFPVHCAESLTGVLE
                     FFSPEIREPDVELLRMMEAIGGQIGQFIERENAQEEVRTSEARKSAILEAALDCVVSM
                     DGNGRVVEFNPMAEKVFGYRREDVIGKEMAELIIPPALRDRHREGLARYLRTGEGPVV
                     GSRIEITAQRADGTEFPVELAITRVAVPGPPLFTGYLRDITDRAQAEEERRRLLASEQ
                     EARAAAEAAQQRLAFLAVASAQMSSSLDYRETLQTLVDLAVPRMADWCFVDVLQEDNA
                     HVRVALAYADPAQADLAQELQQPSPTGERVGVQTVLKGGRSILVDHYTDDVFDELGVT
                     EERRSLLRRLGPKSHMIVPLQARGRVLGALVLLSTTNVYTQDDLHLAEELARRAAIAI
                     DNARLFQERTHVARTLQKSLLPPTLPEIPGIDLAARYEPAGEANEVGGDFFDAFRWGR
                     RRWAIVIGDVSGKGADAAAVTGLARHTLRAAALAQRSPSAILGVLNEALLADGPKEKF
                     STVVFATLENTDDGVCLTAASGGHPPPLVLRRDGKLEELAVPGTLIGVFKDVELTDQF
                     TYLEPGDMVVFYTDGVTDIPGLEDESQPLLVETFLRAAASSDPRVVADEIVKAAVEVQ
                     NGAPSDDIALIVLRVKYPT"
     gene            complement(3602..5557)
                     /locus_tag="VEV43_10855"
     CDS             complement(3602..5557)
                     /locus_tag="VEV43_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24065.1"
                     /translation="MPGGWTFGARRAMCTVAALSLVIVPFDAARPARAAETPIPFATF
                     ADLHVDYARRHVFVSGGSGTNAVFVFGFDGRQVARIDGLAGAADLLPDGDLLYVALSN
                     ADEIAVVNVKSFDVTDRLEVSPYAQPVYLSKSRDTIYFTHSCSGYMEGEMASVDLSTR
                     VPVPHEDNRLIGCMRHAVVPTDENTMFAWDSSGYAPLRRFNVTARQPVFLDESPGDER
                     FDDLEFSSDGETFYVRNTMYGAGIDQRRLGDYSVVMNYPRAGAAYGLTPDEDRLFATT
                     NDYDTGDLAVYRTGSPAPLTTMELDGAYYSQGILPGALAAGPAGDRVFAVGPRDYYDS
                     NLAFRVIWPQYRVAAGGGSQTVPAAGNGYETWTNGGRRVRNETLLARGGGRTFRVNPL
                     HTSGYGGGIDGNRLVYQIVRGRRSDLRLYDLKKRRQISTLDRMNTRAWEWHPTISGGR
                     VLFSRQRGNLSQVILQPLGRGRARVLATERSRTSALIAGQVNGRYATWTWCGRTCEVV
                     RMNLATGNRLRAPRPARRINYGGSVKRDGTVYYIQSGFGCGTNAAIMRFAGGRVTEID
                     ELGTGKDAFFTYADGATDRVLFDEATCTYRGTGSRWDVLSFRDIPTSRDRGGKPSPEE
                     EAPAELPPLEGEPWFGGLLPGGPRSGT"
     gene            complement(5612..6337)
                     /locus_tag="VEV43_10860"
     CDS             complement(5612..6337)
                     /locus_tag="VEV43_10860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYP24066.1"
                     /translation="MVRRVEGIRAVTYDCWGTLLRDRDWEGAMTKRLGALSHFLDLPE
                     DEARALLDEAWARHDEAWKQVESFGPGRMAAYCLEEKGIFDDDKIAQLTKEFEEASLD
                     AGVVEVDDARETLKALKDKGLGVGLICDTGFTPGRVVRQLLDDAGLLEYFDTLCFSDE
                     VGVPKPGNEIFAKALAELGVRPPEALHVGDLRRTDIAGAHDVGMHAVRFKGVHDDKTD
                     APEAEVCIERHPQILEVLDLVEG"
     gene            complement(6330..6731)
                     /locus_tag="VEV43_10865"
     CDS             complement(6330..6731)
                     /locus_tag="VEV43_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HYP24067.1"
                     /translation="MDGIQAAHRLRLSQADAHYGGGLVAGARLLELFGDLATELCIRS
                     DGDEGLFAGYSDVTFLAPVHAGDFVEVRGAITRTGRTSREMQFEVVRYAVTRPDVSDS
                     AADILDEPEPVARATGTCVVKQDRQRGSGLG"
     gene            complement(6806..6988)
                     /locus_tag="VEV43_10870"
     CDS             complement(6806..6988)
                     /locus_tag="VEV43_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24068.1"
                     /translation="MRPDYAGRTVCTTTGKRRYDSYWEAKAASREIRDTMYEDHGHPY
                     RCESCKGWHLGRSQPF"
     gene            7037..7567
                     /locus_tag="VEV43_10875"
     CDS             7037..7567
                     /locus_tag="VEV43_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HYP24069.1"
                     /translation="MDIDAENVVTAAGGRARLEELYRVQVAPLQRLAFLLTSDHHLAE
                     DITQQAFVRFYRRFFALRDPAAAPAYLRRTVVNLARSHHRRGRRERDHLEVEQRTPET
                     TDDPSLEDHDMVVRAVRSLPYRQRAAIVLRFYEDLTEQQAAEALGCSTAAMKSLTARA
                     MSSLRGMDIRGGTDED"
     gene            7557..>7887
                     /locus_tag="VEV43_10880"
     CDS             7557..>7887
                     /locus_tag="VEV43_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24070.1"
                     /translation="MRTKETIREALQREAARHRVNPALPKATIRKARAARVATAALAL
                     AAVTGLVFGGTAVVGAVREDAVRPAGQGETPPERQVEGAPFLLVGHAGWDVSRADQYS
                     PTEGEMTF"
BASE COUNT         1224 a         2731 c         2776 g         1156 t
ORIGIN      
        1 gcaggaggcg tgggaggaga ccgtccgcat gctcgaggtc taccgcgagg tggcgactga
       61 cgtgctggcg atccccgtgc tcgccggacg caagtccgag tccgagcgtt tcccgggcgc
      121 cgacgagacg ctcccgatcg agggcctgat gcgcgaccgc aaggcgctcc agtgcgggac
      181 gtcgcacttc ctcggccaga acttcagcaa ggcgtacgac gtgcagttcc tgaacaaaga
      241 aggcgagcag gagtacgcgt ggggcacgtc gtggggcttc tcgacgcgca tgatcggggg
      301 gacgatcatg gcgcacggtg acgaccgcgg ccttcgtctc ccgccggcga tcgcgccagt
      361 ccaggtcgtc gtcgtcccga tctacaggaa cgacgaggag cagtcctccg tcctgggggc
      421 cgcggcgaag atccgcgacg ccctcgcggg cgacgggatc aaggtgcgcg tcgacgaccg
      481 cgaccagcac cgccccggct tcaagttctc cgagtgggag ctaaagggcg tgccgataag
      541 gatagagatc ggtccgcgcg acctcgcggc cgaccaggtg acacttgccg accggatcac
      601 cggagacaag gagcaggtac cgatcgacca gacgatcgcg ggtatgcgcg ggcggctgga
      661 cgcgagccag gcggcgttgc tggcggacgc caccgccttc cgcgacgcga acacgcacga
      721 ggccacgacg ttcgaccagc tgaaggaagg cgtcgagtcc gaaggcgggt tctggatagg
      781 gccctggtgc ggcgacgctg cgtgcgagga gaaggtgacc gcggagacaa aagccacgat
      841 ccgcgtgctc ccgctcgaac gcgaggaccc gggggccgca tgtctcgtgt gcggcagctc
      901 ggggaacgag cgcgctacct gggcaaaggc ctactagccg ccgccttcgg ttgctaggct
      961 gcgaatagcc agaatccacg tgagggggcc atggcaagac cctggcgtct gcccaccgcg
     1021 gacgttgcct ttgacgaggc gcaacgcaag atcgaagtcc tggagagcca gctcgaagcc
     1081 tctctcgagg gctctctcgt cgtgtggatg gacggcagca tcgtctcgtt caaccggcgc
     1141 ttcgtcgaga tgtggagcct gcccccggac gcgctcgaga ccgagtctga ccaggacata
     1201 actcagacga tcttcgacca gctcgtcgac ccccgcgggt tcgaggagcg ggtcctcgac
     1261 ctctaccgcc atcccgagag gacgccgcgc gacgagctcc ggctcaagga cggccgcgtc
     1321 ttcgaggcgt acggcacgcc gctccaggac gaggggggcc ggtaccacgg gtgggcgtgg
     1381 ttcttccggg atgtgtccga ccgaaagcgg accgagcagc gcctcgccgc gcagtacgag
     1441 gtcgtgaagg tgctggcgga ggccggctcg ctcgcggaag ccgcgcccag gctgttgcgg
     1501 gccgtcgcgg acgggttcgg gtgggcctgc gggacgctct ggcgggtcga ccacgacacc
     1561 gagatgctgc ggtgtgtcga cgtctacacg cagacgggca cggacacgca gtcgttcgtc
     1621 gaggtgagcc gggccttgct ggtcccgcgt ggcggcggcc tgcccgggat cgtatgggag
     1681 tcgggcaagc cggagtgggt gacgaacgtc cacgagaacc cggacttcgt gcgctcctcg
     1741 gcagcccggc aagccgggct ccacgccgcg atcctcttcc ccgtccattg cgcggagagc
     1801 ctcaccggcg tgctcgagtt cttcagcccc gagatccggg agcccgacgt cgagctcctg
     1861 cggatgatgg aggcgatcgg gggccagatc ggccagttca tcgagcgcga gaacgcccag
     1921 gaggaggtcc gcacgagcga ggcgcgcaag tcggcgatcc tcgaagcggc cctcgactgc
     1981 gtcgtgtcga tggacggcaa cggccgcgtg gtcgagttca acccgatggc ggagaaggtc
     2041 ttcgggtacc ggcgcgagga cgtgatcggc aaggagatgg ccgagctgat catcccgccg
     2101 gcgttgcgcg accggcaccg tgaagggctc gctcgctacc tcaggacggg cgaaggcccc
     2161 gtggtcggga gccgcatcga gatcaccgcg cagcgcgccg acggcacgga gtttccggtc
     2221 gagctcgcga tcacgcgggt agccgttccg ggtcctccgc tgttcaccgg gtacctccgg
     2281 gacatcaccg acagggcgca ggccgaagag gagcggcgcc gcctcctggc gagcgagcaa
     2341 gaggctcggg cggctgcgga ggccgcgcag cagaggctcg cgttcctcgc ggtcgccagc
     2401 gcccagatgt cctcgtcgct cgactaccgg gagaccctcc agacgctcgt cgacctggcg
     2461 gtcccgcgga tggcggactg gtgcttcgtc gacgtgctgc aggaagacaa cgctcacgtg
     2521 cgggtcgccc tcgcctacgc cgatcccgcg caggcggacc tggcacagga gctgcagcag
     2581 ccctccccca ccggcgagcg cgtcggcgtc cagaccgtgc tgaagggggg ccgctcgatc
     2641 ctcgtcgacc actacaccga cgacgtgttc gacgagctcg gcgtcacgga ggaacggcgc
     2701 agcctgctca ggcggctggg gccgaagtcg cacatgatcg tgccgctgca ggcgcgcggc
     2761 cgggtcctcg gggccctggt gctgctgtcg accaccaatg tctacacgca ggacgacctc
     2821 cacctcgccg aggagctggc gcgccgggcc gccatcgcga tcgacaacgc ccgcctcttc
     2881 caggagcgga cgcacgtggc gcgcaccttg cagaagagcc tgctgccccc gacgctcccg
     2941 gagatccccg ggatcgacct cgccgcgcgc tacgagcccg cgggcgaggc gaacgaggtc
     3001 ggcggcgact tcttcgacgc gttccgctgg ggtcgccgcc gctgggcgat cgtgatcgga
     3061 gacgtctccg gcaagggcgc ggacgcggcc gcggtgaccg gcctcgcgcg gcacacgctg
     3121 cgcgccgcgg ccctcgcgca gcgaagcccg agcgcgatcc tcggcgtcct caacgaggcc
     3181 ctgctggccg acggtccgaa ggagaagttc tcgacggtcg tgttcgcgac gctcgagaac
     3241 accgacgacg gcgtctgcct caccgcggcg agcgggggcc acccccctcc gctggtgctg
     3301 cgccgcgacg gcaagctcga ggagctcgcg gtcccgggca cgctgatcgg cgtcttcaag
     3361 gacgtcgagc tcaccgacca gttcacctac ctcgagcccg gcgacatggt cgtcttctac
     3421 acggacggag tgaccgacat cccggggctc gaggacgaga gccagccgtt gctcgtcgag
     3481 acgttcctgc gggcggcggc atcgtccgac ccgcgtgtgg tggcggacga gatcgtgaaa
     3541 gcggccgtcg aggtgcagaa cggagcgccc agcgacgaca ttgcgctgat cgtcctgcgc
     3601 gtcaagtacc cgacctaggc ccccccggga gcaggccccc gaaccacggc tcgccctcca
     3661 gcggcggcag ctcggccggc gcctcttcct ccggcgacgg cttgcctccg cggtcccggc
     3721 tcgtcgggat gtcccggaac gagaggacgt cccatctgct ccccgtcccg cgataggtgc
     3781 acgtcgcctc gtcgaacagg acccggtcgg tcgcgccgtc cgcgtacgtg aaaaacgcgt
     3841 ccttgccggt cccgagctcg tcgatctcgg tgacgcggcc cccggcgaag cgcatgatcg
     3901 cagcgttcgt cccgcagccg aagcccgact gtatgtagta gacggtcccg tccctcttga
     3961 ccgagcctcc gtagttgatg cggcgtgcgg gacggggcgc ccgcagtcga ttgcccgtcg
     4021 cgaggttcat ccggacgacc tcgcacgtgc ggccgcacca agtccacgtg gcgtagcgcc
     4081 cgttcacctg gccggcgatg agcgcgctcg tccggctccg ctccgtcgcg agcacccggg
     4141 cccgcccccg cccgaggggc tggaggatca cctgagacag gttcccccgc tggcgcgaga
     4201 agaggacgcg tccccccgag atcgtcgggt gccactccca cgcgcgcgtg ttcatgcggt
     4261 cgagcgtcga gatctgccgg cgcttcttca ggtcgtagag ccgtaggtcc gaccggcggc
     4321 cgcgcactat ctgatacacg agccggttcc cgtctatgcc gcccccgtac cccgaggtat
     4381 gcaacgggtt cacccggaag gtccgcccgc ccccgcgcgc gagcagcgtc tcgtttctca
     4441 ccctgcgccc gccgttcgtc caggtctcgt agccgttccc tgccgccggg accgtctgcg
     4501 agccgccgcc cgccgcgacg cggtactggg gccagatcac cctgaacgcg aggttggagt
     4561 cgtagtagtc cctgggcccg acggcgaaga cgcggtcgcc ggcgggcccc gcggcgagag
     4621 ctccggggag gatcccctgc gagtagtagg caccgtcgag ctccatcgtg gtcagcggag
     4681 cgggcgagcc cgtgcggtat acggcgaggt cccccgtgtc gtagtcgttc gtcgtcgcga
     4741 acaagcggtc ctcgtcgggg gtcagcccgt acgcggcgcc cgcgcgcggg tagttcatga
     4801 cgacgctgta gtcaccgagg cgccgctggt cgatccccgc cccgtacatg gtgttgcgga
     4861 cgtagaacgt ctccccgtcg gaggagaact cgaggtcgtc gaagcgctcg tccccgggtg
     4921 actcgtccag gaagaccggc tggcgcgccg tgacgttgaa cctacggagc ggtgcgtagc
     4981 cggaggagtc ccacgcgaac atcgtgttct cgtccgtcgg cacgacggcg tgcctcatgc
     5041 acccgatcag acggttgtcc tcgtgcggca cgggcactcg tgtcgagagg tcgaccgacg
     5101 ccatctcccc ctccatgtac ccgctgcacg aatgcgtgaa gtagatggtg tcccggctct
     5161 tgctcagata gaccggctgc gcgtacggcg acacctccag cctgtcggtg acgtcgaagg
     5221 acttcacgtt cacgaccgct atctcgtccg cgttcgagag agccacgtag agcaggtcgc
     5281 cgtcggggag gaggtcggct gcgccggcga ggccgtcgat ccgcgcgacc tggcggccgt
     5341 cgaagccgaa gacgaagacc gcgttggtcc cggagccccc cgacacgaag acgtgacgcc
     5401 gcgcgtagtc gacgtgcagg tcggcgaacg tggcgaacgg gatgggggtc tcggccgcgc
     5461 gcgccggacg ggccgcgtcg aacggcacga tcacgagcga gagcgccgcg acggtgcaca
     5521 tcgcccgccg cgcgccgaag gtccaccccc ctggcacgag gcaatgtaag caggccgggc
     5581 cgggtgtcgc tagggggttg gcccgaaaaa gctagccctc tacgaggtcc agcacctcga
     5641 ggatctgcgg gtggcgctcg atgcacacct cggcctcggg ggcgtcggtc ttgtcgtcgt
     5701 ggacgccctt gaaccggacc gcgtgcatgc cgacgtcgtg cgctccggcg atgtccgtgc
     5761 gcctcaggtc gccgacgtgc agcgcctccg gcggacggac cccgagctcc gccagagcct
     5821 tggcgaagat ctcgttgccc ggcttcggca cgccgacctc gtccgagaag cacagggtgt
     5881 cgaagtactc gagcaggccg gcgtcgtcga ggagctggcg gaccacccgg ccgggggtga
     5941 agccggtgtc gcagatgagg ccgaccccga ggcccttgtc cttcagcgcc ttcagggtct
     6001 cgcgtgcgtc gtcgacctcg acgactccgg cgtcgaggct cgcctcctcg aactccttcg
     6061 tgagctgggc gatcttgtcg tcgtcgaaga tccccttctc ctcgaggcag taggcggcca
     6121 tcctgccggg gccgaagctc tcgacctgct tccacgcctc gtcgtggcgc gcccaggctt
     6181 cgtcgaggag cgcacgggct tcgtcctcgg ggaggtccag gaaatgactg agggcgccca
     6241 gacgtttcgt catggcgccc tcccaatcgc ggtcccgcag caacgtgccc cagcagtcgt
     6301 aggtgactgc tcggatcccc tccacccttc taaccaaggc cactccccct ttgccggtcc
     6361 tgcttcacca cgcacgttcc cgtagctctg gccacgggct cgggctcgtc gaggatgtcg
     6421 gcggcggagt ccgagacgtc gggccgggtc acagcgtacc ggacgacctc gaactgcatc
     6481 tcccgggaag tgcggccggt tcgggtaatc gcccctctga cctcgacgaa gtcgccggcg
     6541 tggacggggg ccaggaaggt gacgtccgag tagcccgcga agagcccctc gtccccgtcc
     6601 gagcggatgc agagctcggt ggcgaggtcg ccgaagagct cgaggagccg ggccccggcg
     6661 acgaggccgc cgccgtaatg ggcgtcggcc tgcgagagcc ggagccgatg cgcagcctgg
     6721 atgccgtcca cgcgtggagc gtagcccggc cgggcgcagt cgagtgagga aaatcggacg
     6781 ctattcgtcc gtttttcctc aacggtcaga agggctggct ccggcccagg tgccagccct
     6841 tgcaggactc acatcggtac gggtggccgt ggtcctcgta catcgtgtcg cggatctcgc
     6901 gcgaggctgc cttcgcctcc cagtacgagt cgtagcggcg cttccccgtc gtcgtgcaga
     6961 cggtgcgtcc tgcgtagtcg ggcctcaccg ggcgacacta ccgacaccta accttcgaac
     7021 ggggcgtcta taggtcatgg acatcgacgc tgagaacgtc gtgacggcgg ccgggggccg
     7081 ggcccggctc gaggagctct atcgcgtgca ggtggcgccc ctgcagcggc tcgcgttcct
     7141 cctgacgagc gaccaccacc tcgccgagga catcacgcag caggccttcg tcaggttcta
     7201 ccggcgcttc ttcgcgctgc gagacccggc ggcggccccc gcgtacctgc ggaggacggt
     7261 cgtcaacctc gcccgctcgc accaccgacg ggggcggcgc gaacgcgatc acctcgaggt
     7321 cgagcagcgg acacccgaga ccacggacga tcccagcctc gaggaccacg acatggtcgt
     7381 ccgggcagtg aggtcgctcc cctaccggca gcgggccgcg atcgtcctgc gcttctacga
     7441 ggacctgacg gagcagcagg cggccgaggc actgggctgc tcgaccgccg cgatgaagtc
     7501 gctgaccgcg cgggcgatgt cgtcgctgcg gggcatggac atccgaggag gaaccgatga
     7561 ggactaaaga gacgatcagg gaggccctcc agcgagaggc ggcccggcac cgtgtgaacc
     7621 ccgccttgcc gaaggcaacg atccgcaagg cgcgggctgc ccgggtcgcg accgcagccc
     7681 tcgcgctcgc cgcggtgacc ggcctcgtct tcggcggcac ggcggtcgtc ggagcggtcc
     7741 gggaggacgc ggtgcggcct gcgggtcaag gcgagacccc gcccgaaaga caggtcgaag
     7801 gcgcgccgtt cctgctcgtc ggccacgcgg gctgggacgt ctcacgagcc gaccagtaca
     7861 gccccaccga gggcgagatg acgttca
//
LOCUS       DASQDO010000385         5726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_530068, whole genome shotgun sequence.
ACCESSION   DASQDO010000385 DASQDO010000000
VERSION     DASQDO010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530068"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VEV43_10885"
     CDS             complement(<1..881)
                     /locus_tag="VEV43_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24071.1"
                     /translation="MRRVLNLALAALLLAVPATAMARQKGPNVVIAKDPKGSFELVGH
                     EPLLNRGMNAAIAVHGDYAYVGSRTDGLHPRSGVAIVDVSDPANPTEVGQIGPPNEGN
                     VGETSREMRIWADKDLLIVLNLASNCSFIIHACSPTQAVGTDNYRFYDISGDKAAAPE
                     LVATYEPSKDPHEFYLWQDPKDPDRALLFQSTPGGGQTSLLVTDISNYAKGEFKEIGE
                     WNTLIPSPETDNRLHSLAVSPDGTKAYLSYLGGGFFMVDTSDFAKGVAKPKVKPLTPI
                     QNRVSWGDPGVHSAVPFP"
     gene            complement(985..1296)
                     /locus_tag="VEV43_10890"
     CDS             complement(985..1296)
                     /locus_tag="VEV43_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24072.1"
                     /translation="MDRDENLAILPFDDPNAAPFVVFLTEEQVKFSWQLIECDGTRLT
                     KGEAAVRLMEHLKPTRWLGKLCRPRLITKLVGAIDHLISISRPKLSHLVPQGPGPRRW
                     P"
     gene            1474..2790
                     /locus_tag="VEV43_10895"
     CDS             1474..2790
                     /locus_tag="VEV43_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018812287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24073.1"
                     /translation="MPKRLFVLLLACAALAAGALPAAAAPASPSFGPVIDPVGGYEGQ
                     TKCSPDAKPGVVAFQQMVLAEYPGTGAGGIGRDCSYGGQSEHKEGRAWDWGVSAADAS
                     HREAVADLMSWLSATDRYGNEHALARRLGIMYVIWNKRIWFPFSGWRTYCEMKKGACV
                     GDDGGARSPHTDHVHFSFTWAGARKETSFWHAERSLVGAIAGNPSGLGYWLSGRTGSV
                     FGFGTGDYGSLEGSYSQKPVEALEPTPSGYGYWILSRAGRVRAFGDAPYKGSRTEGSA
                     PVVDMAALPTGRGYWLVSRGGRVYSFGRARGFGGLQDSGFSGDVVAIEATPTGAGYWL
                     FGSNGAVFAFGDAKDFGGAANDGVSGAVVDAEPFGAEGYWMVTESGRVLAFGAAPDLG
                     GAAGKSFAGEVVGMAAAPGGTGYWLATDRGQVLSLGGVRSLGSLVR"
     gene            complement(3549..3809)
                     /locus_tag="VEV43_10900"
     CDS             complement(3549..3809)
                     /locus_tag="VEV43_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24074.1"
                     /translation="MADGESRQEQLNLRLSRAHYDILDAVAYLDETNRASVVRTVIEE
                     YLQDRLGEEDVQLLLAIRKRHRGLDAMRSPTREGASAKLNPD"
     gene            complement(4067..4774)
                     /locus_tag="VEV43_10905"
     CDS             complement(4067..4774)
                     /locus_tag="VEV43_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24075.1"
                     /translation="MEIQMKRAFAVSLMVAVVATSLLGPAEAAKKKKPKKPPAPAPVQ
                     VDATYFLRRDGETCEDASLFLSLVDAEDVDPGSCGNWTMGILAPALTAAEQQATPLHY
                     ATREADGVPVTLDATKDITGLIGVKSTTSIPFANVATPVRWGLGQTTLNVELLGTLAD
                     GTEKTIGSTTVDYDVTPGDAAQLYEVEFSMKPDAALDKMAFTKLTLRLLNSGNSIGHG
                     YYTTDNPASHFKMGTWK"
     gene            complement(4868..4940)
                     /locus_tag="VEV43_10910"
     tRNA            complement(4868..4940)
                     /locus_tag="VEV43_10910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4905..4907),aa:Ala,seq:tgc)
     gene            complement(5023..5634)
                     /locus_tag="VEV43_10915"
     CDS             complement(5023..5634)
                     /locus_tag="VEV43_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24076.1"
                     /translation="MRKIVIFMLVGLLGVAVLGPAAAKKKKPKKPAAPVPVELQYFLR
                     APESCTTDSFLSLTDGTDADCVYVDSATNAVATAAGLDAWMHYPAVDGLPLTLDTARK
                     VVATIATRGANGTGGGPTSFEFVLVGTVAGEEKELARHTESGTFGPGEIKTMEFEMTL
                     DPALAGAVLEGLRLDVYVEGPALVGRGIEHDEPVSSIKVPALK"
BASE COUNT          829 a         1864 c         2050 g          983 t
ORIGIN      
        1 gggaacggca ccgcgctgtg cacgccgggg tcgccccagc tgacgcggtt ctggatcggc
       61 gtgagcggct tcaccttcgg cttggcaacg cccttcgcga agtccgacgt gtcgaccatg
      121 aagaagccgc cgcccaggta ggagaggtac gccttcgtgc cgtcggggct gacagccagg
      181 gagtgcaggc ggttgtcggt ctcggggctc gggatcagcg tgttccattc gccgatctcc
      241 ttgaactcgc ccttggcgta gttcgagatg tcggtgacga ggagcgacgt ctggccgccg
      301 ccgggagtcg actggaacag cagcgcacgg tcggggtcct tcgggtcctg ccagaggtag
      361 aactcgtgcg ggtccttgct cggctcgtac gtagcgacga gctcgggggc cgccgccttg
      421 tcaccggaga tgtcgtagaa gcggtagttg tcggtgccga ctgcctgcgt cggcgagcac
      481 gcgtggatga tgaagctgca gttgctcgcg aggttcagga cgatcagcag gtccttgtcg
      541 gcccagatcc gcatctcacg cgaggtctcg ccgacgttgc cctcgttcgg cgggccgatc
      601 tggccgacct cggtcgggtt cgcggggtcc gacacgtcga cgatcgcgac gcccgagcgc
      661 gggtggaggc cgtcggtacg gctgcccacg tacgcgtagt ccccgtggac ggcgatcgcc
      721 gcgttcatcc cgcggttgag caggggctcg tgcccgacga gctcgaacga ccccttcggg
      781 tccttggcga ttacgacgtt ggggcccttc tggcgggcca tggccgtggc gggaaccgcc
      841 aggagcaggg ccgcgagggc gaggttgaga acgcgacgca cgtgaaccag actccttaga
      901 tcgccgaatc cgcaatgcca tggagcaccg ccgaggcgat gcttcgtggt ccaagggttc
      961 gacggtcccg gacgcttccc tccgtcaggg ccagcggcgg gggccgggtc cctgcggcac
     1021 gaggtggctc agcttcggcc ggctgatcga gatcaggtgg tcgatcgcgc cgacgagctt
     1081 ggtgatgagg cgggggcggc agagcttgcc gagccagcgg gtcggcttga ggtgctccat
     1141 gaggcgtacg gccgcctcgc ccttggtcag ccgggtcccg tcgcactcga tgagctgcca
     1201 cgagaacttg acctgctcct cggtgaggaa cacgacgaag ggggccgcgt tcgggtcgtc
     1261 gaacggcagg atcgccaggt tctcgtcccg gtccatcttg gagacgacac gcgcccccca
     1321 gcaacagaag cgtcagccgt cttcgtagag caggagcggg cgctggagct cgccggctct
     1381 cttgaccgct ccggcttcgc cgctcaatgc atcgatggac acgggcgagg cttaccccta
     1441 aggaggcgcg gtcactcggc cgatggacca atcatgccca aacgcctctt cgtcctcctg
     1501 ctcgcgtgcg ccgcgctcgc ggcgggggcg ctgccggcgg ccgcggcccc cgcgtccccg
     1561 agcttcggcc ccgtgatcga tccggtcggc ggctacgagg gtcagacgaa gtgctctccg
     1621 gacgccaagc ccggggtcgt cgcgttccag cagatggtgc tcgccgagta cccggggacg
     1681 ggggccggag gcatcggtcg cgactgttcc tacggtggcc agagcgagca caaggagggc
     1741 cgagcctggg attggggcgt gagcgcggca gacgcttccc accgcgaggc ggtcgccgac
     1801 ctcatgtcgt ggctgtccgc caccgaccgc tacgggaacg agcacgctct cgcccggcgg
     1861 ctcgggatca tgtacgtgat ctggaacaag cgcatctggt tcccgttctc gggatggcgc
     1921 acctactgcg agatgaagaa gggcgcgtgc gtcggcgacg acgggggagc gcgctcgccc
     1981 cacaccgacc acgtccactt cagcttcacg tgggccggag cgcgcaagga gacgtcgttc
     2041 tggcacgccg agcgctcgct cgtcggggcg atcgccggta atccgtcggg gctcggctac
     2101 tggctctcgg gccggaccgg ctcggtcttc ggcttcggca cgggcgacta cgggtcgctc
     2161 gagggttcgt actcgcagaa gccggtcgag gccctcgagc cgacgccctc gggatacggc
     2221 tactggatcc tctcccgggc cggacgcgtg cgcgccttcg gcgacgcgcc gtacaagggg
     2281 agcaggaccg agggctctgc gccggtcgtc gacatggcgg cgctcccgac gggacgcggc
     2341 tactggctcg tgtcgcgtgg gggccgcgtg tactcgttcg gccgggctcg cgggttcggg
     2401 ggcttgcagg actccggctt ctccggagac gtcgtggcga tcgaagcgac tccgacgggg
     2461 gccggctact ggctgttcgg ctcgaacggc gcggtgtttg cgttcgggga cgcgaaggac
     2521 ttcggcggag cggccaacga cggggtgtcg ggtgcggtcg tcgacgcgga gccgttcggg
     2581 gccgagggct actggatggt cacggagtcg ggccgggtcc tggccttcgg ggcggcgccc
     2641 gacctagggg gagccgcggg caagtcgttc gcgggcgagg tcgtcggcat ggcggccgcc
     2701 ccgggcggga cgggatactg gcttgcgacg gaccggggac aggtgctgag cctcggcggg
     2761 gtccggagcc tgggctcgct cgtcaggtag ccgttgccgc ggcctgcggg cctcgaattc
     2821 gatcttcccg gtgtccgagc agcagaggtc tccttcctcg tacggggggt cctcgtcgag
     2881 gtgagcgcgg aggtaggcca cgaacgcgac cgcctccttc tgcagggagc ggaggtcagg
     2941 ccgggggatg acaccgtctc ggctctccca ctgcgccgca tgctcaaaga agggctggtc
     3001 ctcgcagacg tcgagaaccg gcgcgcacgc cgtgaggact ttggtgagac cgtccgccag
     3061 ccgctcctgc tgggccggcg agggggccgg gccgtcgagg atctcgtcga tcacgtcggc
     3121 actagacgta gccgaggcca gcagaaggtc gagcttagcc gggcgatcgt cgggtgcggg
     3181 agggtcgtcg tcgaaatagt ccgtgaagaa tgccgtggcc gctgctgtga tgatcgccag
     3241 gatcgcagcg gcgatgaggc cgcggacgcc ccatctgtcc ttcctccggt tcctattgcg
     3301 ggaaatggtt tgcacctccg cgttgatcaa tcgagcgagg acacagtccg ggcgtcgaca
     3361 gatcacgggc gggagagccg gcggttccgg gctggttgga atgttcgccg cgacgccttc
     3421 cattcctccc tacaagcgcg tgattcccca ggtcagcggg ggtttgcgcc gtctacaaag
     3481 tagctcgcta ctcgtcggag gtcggcccca tacgggctcc agaatcctat tttggcggcg
     3541 ccgtcggatc agtcagggtt cagtttggcc gacgccccct cgcgcgtggg ggagcgcatc
     3601 gcatcgagtc cccggtggcg cttgcggatg gctaggagga gctggacgtc ttcttccccg
     3661 agacggtctt gcaggtactc ctcgatcacg gtgcggacga ccgacgcccg attcgtctcg
     3721 tcgagatagg cgacggcgtc gaggatgtcg tagtgggcgc gggacagccg aaggttgagc
     3781 tgctcttggc gtgactcccc gtcggccaaa tccgtatcct ctccgtcgct acatagtagc
     3841 gtgttactta gccgaattct acgaaaaggc cccttgtcct gccgggggct tgttcgtgcg
     3901 tcgaccgcgc tgcgtcccac cggaggaaaa ggagcgggtt agaagccccg gtccaagctt
     3961 cgggcaggca cgcgtggcgg ggtgggcagc ctgggctccc tcgtcggtag ccgcaaagaa
     4021 aagagccggc cccgaagggc cggcccctct cggaactctc cgcgatctac ttccaggtcc
     4081 ccatcttgaa atgcgaggcc gggttgtccg tcgtgtagta gccgtggccg atgctgttgc
     4141 cgctgttgag caggcgcagc gtcagcttcg tgaacgccat cttgtcgagg gccgcgtccg
     4201 gcttcatcga gaactcgacc tcgtagagct gcgccgcgtc gcccggcgtg acgtcgtagt
     4261 cgaccgtggt gctgccgatc gtcttctcgg tcccgtccgc aagcgtcccg aggagctcga
     4321 cgttcagggt cgtctggccc aggccccacc gcaccggggt cgcgacgttg gcgaagggga
     4381 tgctcgtcgt cgacttcacg ccgatcagcc cggtgatgtc cttggtggcg tcgagcgtga
     4441 ccgggacgcc gtcggcctca cgggtcgcgt agtgcagcgg cgtcgcctgc tgctcggccg
     4501 cggtcagcgc gggggccagg atgcccatcg tccagttccc gcacgagccg gggtcgacgt
     4561 cctcggcgtc gacgaggctg aggaacagtg acgcgtcctc gcaggtctcg ccgtcgcggc
     4621 gaaggaagta ggtggcgtcg acctggacgg gggccggggc cgggggcttc ttcggcttct
     4681 tcttcttggc cgcctcggcg gggccgagga gcgaggtggc gacgacggcc accatcaggg
     4741 atacggcgaa agcgcgcttc atctggatct ccattcctat gccgccttga cggaatggag
     4801 tgttcgcctt gctgtaagac ggtcctgcga ccggcggtga cgttgcgtag tcacgatctc
     4861 gactaagtgg agcggagcgg gctcgaaccg ccgaccccct gcttgcaaag caggtgctct
     4921 accaactgag ctaccgcccc tcgagcccgt aagtctaggc gaagcctccg acgcgaaccg
     4981 gccgcgggca gtccccgcgg ccggtctcga acgtcacagt ggctacttca gcgccgggac
     5041 cttgatcgac gacaccggct cgtcgtgctc gatcccgcgg ccgaccagcg ccgggccctc
     5101 gacgtagacg tcgagcctca gcccttcgag gaccgcgcct gcgagcgccg ggtccagcgt
     5161 catctcgaac tccatcgtct tgatctcacc cgggccgaac gtgcccgact cggtgtgcct
     5221 cgccagctcc ttctcttcgc cggcgaccgt gccgaccagc acgaactcga acgacgtcgg
     5281 ccccccgccg gtgccgttgg cgccgcgggt cgcgatcgtc gcgacgacct tcctcgccgt
     5341 gtcgagcgtc aacgggaggc cgtcgaccgc cgggtagtgc atccaggcgt cgagccccgc
     5401 cgcggtggcg acggcgttgg tggcgctgtc gacgtagacg cagtcggcgt cggtaccgtc
     5461 ggtcaggctg agaaaggagt cggtagtgca gctctcgggg gcccgcagaa agtactggag
     5521 ctcgaccggg accggcgccg caggcttctt cggcttcttc ttcttggccg cggccgggcc
     5581 gagcacggcc actcccaaca gccccacgag catgaagatc acgatctttc gcatggctcc
     5641 cccttggtcg acgtctcccc agacctaagc cttacgggac gcgtcgacga tctcctgccg
     5701 acgaatcgcc gcgaactccc aaaatc
//
LOCUS       DASQDO010000386         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_163784, whole genome shotgun sequence.
ACCESSION   DASQDO010000386 DASQDO010000000
VERSION     DASQDO010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163784"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1698)
                     /locus_tag="VEV43_10920"
     CDS             complement(43..1698)
                     /locus_tag="VEV43_10920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HYP24077.1"
                     /translation="MTQLGRRSTALERLSGGTFDLLVVGGGITGAGIALDAAARGMSV
                     ALVERGDFASGTSSKSSKLVHGGLRYLEQREFGLMREASVERDLLRRLAPHLVEPIPF
                     VLPVSSRWNRALFGAGLWTYDALASFKNLKVHRHLDGDETEHLVPAMPKGKARGGYLF
                     YDCRTDDVRLVLAVLGEARRYGAAVANYAAVESLDGGEDTCRAAVRDRVSGDLFEVSA
                     RRAVVAAGVWGDRLEQMSRTGAEARLRPSKGIHLVFRRDDLPVGEAAAFIPDAERKRM
                     LFVIPWHDAVLVGTTDTAYEGDLDAPAVEPEDRAYCLDAVNSTFGLDLGDGDIAGAYA
                     GLRPLIAARAGATADLSRRHAVYSIAPGVTGITGGKLTTWRRMAKDAVDGVAAELGVR
                     SKCRTQWIRLGSSDVSRLRAAVGRRAARLGIAQARVDNLVRLYGDSALAVLDVAERDG
                     LAGPLNGESPSLAAEAVYCAESEMVVHLDDFLARRTRHALTDPPAGTGAASVAPELVA
                     AALGWGEGQKASEVDRHRALVERERGTRLHEEHQPPSAHLGAG"
     gene            complement(1695..1994)
                     /locus_tag="VEV43_10925"
     CDS             complement(1695..1994)
                     /locus_tag="VEV43_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24078.1"
                     /translation="MKIEDRQGDSLSEATVTVDDEELIDLLQGLADVVEGKREHLHFS
                     QVGGPQLVVRRTAEADDDPLGRQLDWWMGPLILFGLVFVVIGAATVIRWAAGLIS"
     gene            2013..3158
                     /locus_tag="VEV43_10930"
     CDS             2013..3158
                     /locus_tag="VEV43_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP24079.1"
                     /translation="MPADLRLLDEEQRDLAAMVREFATKEAGPASIGYEERSEDPVAL
                     YQQLAELGLTGISFPEEYGGGGQPYGTYLLVIEELARSYLALAIGLSVHTLCAFAVNE
                     FGTDAQRKDVLSKLCSGEWYGAYSLSEPGSGSDAAALTSKAVRSDGGYLLNGSKVFCT
                     RGAEADAVLMMARTGDESARGISAFILRRGTSGFAGSKKEDKMGWRSSPTWMLQMEDV
                     HVDEGDRIGEEGQGFKIALASLDSGRLGIAACAVGVAQAALDEALTFTREREQFGKPV
                     ATNEGLQFMLADMATQIEAGRRLYRHAAALRDAKQDYSLQAAQAKLFCTDMAMKVTTD
                     AVQLHGGYGYITEYPVERFMREAKGLQIVEGTNQIQRYVIGRKLTGL"
BASE COUNT          538 a         1260 c         1229 g          503 t
ORIGIN      
        1 aggagcgaac gatgtctacc tcgtggtcga gatgcggccc tctcatccgg ccccgaggtg
       61 agcggacggc ggctggtgct cctcgtgcag gcgggtgccg cgctcgcgct cgacgagcgc
      121 ccgatgacgg tcgacctccg aggccttctg cccctcgccc cacccgagcg ccgccgcgac
      181 gagctcgggc gcgaccgatg cggcccccgt ccccgccggg ggatccgtca gcgcgtgccg
      241 cgtccgccgg gccaggaagt cgtccaggtg cacgaccatc tcggactcgg cgcagtagac
      301 ggcttccgct gccagcgagg gcgactcgcc gttcagaggg ccggccaggc cgtcgcgctc
      361 ggcgacgtcg agcaccgcga gcgccgaatc cccgtacaga cggacgaggt tgtcgacgcg
      421 tgcctgcgct atgccgagtc gcgccgccct ccggcccacc gccgcgcgca ggcgggagac
      481 gtcgctcgag ccgaggcgga tccactgcgt tcggcacttc gaccggacgc cgagctccgc
      541 ggcgacgccg tcgaccgcgt ccttcgccat ccgtcgccag gtcgtgagct tgccgccggt
      601 gatccccgtg acgcccggcg ctatggagta caccgcgtgg cgccgcgaca ggtctgcggt
      661 cgctccggcc cgggccgcga tcaacgggcg cagtcccgcg taggcgccgg ctatgtcgcc
      721 gtcgccgaga tcgaggccga acgtcgagtt gaccgcgtcg aggcagtagg cgcggtcctc
      781 gggctcgacc gcgggcgcgt cgaggtcgcc ctcgtacgcg gtgtcggtgg tcccgacgag
      841 cacggcgtcg tgccacggga tgacgaacag catcctcttc cgctccgcgt cggggatgaa
      901 agcggcggcc tcgcccacgg gaaggtcgtc cctgcggaag acgaggtgta tgcccttcga
      961 gggacgaagc cgcgcctcgg cccccgtccg cgacatctgt tcgagccggt cgccccacac
     1021 tccagccgcg acgaccgcgc ggcgcgccga gacctcgaac aggtcgccgg agacgcggtc
     1081 gcgcacggcc gcgcggcacg tgtcctcgcc gccgtcgagg gattcgaccg ccgcgtagtt
     1141 cgcgacggcc gcgccgtagc ggcgagcctc cccgagcacc gcgagcacga gccgcacgtc
     1201 gtcggtcctg cagtcgtaga agaggtagcc gccgcgcgcc ttgcccttcg gcatcgccgg
     1261 cacgagatgc tcggtctcgt cgccgtcgag gtgccggtgg accttgaggt tcttgaagct
     1321 cgccagggcg tcgtaggtcc acaggcccgc accgaacagc gcgcggttcc agcgactcga
     1381 caccggcagc acgaacggga tcggctcgac gaggtgcggg gcgagccggc gcagcaggtc
     1441 gcgctcgacc gacgcctcgc gcatgaggcc gaactcccgt tgctcgagat agcgcagacc
     1501 gccgtggacg agcttcgacg acttgctcga cgtccccgac gcgaagtcgc cccgctcgac
     1561 gagcgcgacc gacatcccgc gcgcggccgc gtcgagcgcg atccccgcgc cggtgatccc
     1621 gcccccgacg acgagcaggt cgaacgtccc gcccgacagc cgctcgagcg cggtgctccg
     1681 gcgcccgagc tgcgtcacga gatcagtccg gccgcccagc ggatgaccgt cgcggccccg
     1741 atgacgacga acacgaggcc gaacaggatc agcggtccca tccaccaatc gagctgacgg
     1801 cctagtggat cgtcgtcggc ctcggccgtc ctgcggacga cgagctgcgg ccctccgacc
     1861 tgcgagaagt gcaggtgctc gcgctttccc tcgaccacgt cggcgagccc ctggagcagg
     1921 tcgatgagct cctcgtcgtc gacggtcacg gtcgcttcgg agagcgaatc tccctggcgg
     1981 tcctcgatct tcatgcgtat atcctcctgc acatgcccgc cgacctgcgc ctgttggacg
     2041 aggaacagcg cgacctcgcg gcgatggtac gggagttcgc tacgaaggaa gccgggcccg
     2101 cgtcgatcgg gtacgaggag cgttcggagg acccggtcgc cctctaccag cagctcgccg
     2161 agctcggcct gaccggcatc tcgttccccg aggagtacgg cggtggggga cagccctacg
     2221 ggacgtacct gctcgtgatc gaggagctcg cgcgctcgta tctggccctc gcgatcggcc
     2281 tgtcggtgca cacgctgtgc gcgttcgccg tcaacgagtt cggcacggac gcgcagcgca
     2341 aggacgtcct ttcgaagctc tgcagcggcg agtggtacgg cgcgtactcg ctgtccgagc
     2401 cgggctccgg gtccgacgcc gccgcgttga cgagcaaggc cgtccgttcc gacggcgggt
     2461 atctcttgaa cgggagcaag gtcttctgca cccggggggc cgaggccgac gccgtgctga
     2521 tgatggcgcg taccggcgac gagtcggcgc ggggcatctc cgcgttcatc ctgcgcaggg
     2581 ggacttcggg cttcgccggc tcgaagaagg aagacaagat gggctggcgc tcgtcgccga
     2641 cgtggatgct ccagatggag gacgtgcacg tcgacgaggg cgaccgcatc ggcgaggagg
     2701 gccagggctt caagatcgcg ctcgcgtcgc tcgactccgg ccgtctgggg atcgcggcgt
     2761 gcgcggtcgg cgtcgcgcag gcggcgctcg acgaggcgct gacgttcacg agggagcgcg
     2821 agcagttcgg caagccggtc gccacgaacg agggtctcca gttcatgctc gccgacatgg
     2881 cgacccagat cgaggccggc cgacgtctct accgtcacgc cgcggccctg cgggacgcca
     2941 agcaggacta ctcgctgcaa gccgcccagg cgaagctgtt ctgcaccgac atggcgatga
     3001 aggtgacgac ggacgcggtg cagctccacg ggggctacgg gtacataacg gagtacccgg
     3061 tagagcgctt catgcgtgag gcgaagggcc tgcagatagt cgagggcacg aaccagatcc
     3121 agcgctacgt cataggccgc aagctcaccg gtctgtagcc cggcgccccc ctggcccgga
     3181 tgggcagcag agcgagttct ttgctataac ccgtccggca tatgtctgac atgtctgact
     3241 tgtccgacgc ggaccggacc agggggatgg atgaagcggg cgcccggcac ggcagctagg
     3301 ccgttcggcc cagatttacc gcttgtgacg caccgaggca ggattccgcc accgccgctc
     3361 gaaatcgtca ccgtccgaac cgccgccagc taggaggaca cccttgagac gcatccgact
     3421 gctagtcgtc gccgccctgg cgaccgcctc gctcggcatc gccgcgcccc ccgcgtccgc
     3481 gtgcccgaac gaggatcagc ctccctgcgc gtgcgaaaac gccaacgact
//
LOCUS       DASQDO010000387         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_469386, whole genome shotgun sequence.
ACCESSION   DASQDO010000387 DASQDO010000000
VERSION     DASQDO010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469386"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VEV43_10935"
     CDS             <1..662
                     /locus_tag="VEV43_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP24080.1"
                     /translation="EVDVTHGGKTETLGAEVLLLAAGRTPATADAGLEHAGVERDGAY
                     VVTRDDALETTSPGISAVGDLLGPPAPARANSAFAEGMLVAERIAGIDGPGIDYAQVP
                     RVTHGMIETACVGLSEERAAAEGLEPRAAAIQTTGVAMGLISGDPGLAKVVTDGDGTV
                     VGVHLVGPHATELIPGAAAVTSFEATASEAASIVHAHPTLAESMQELYLALSGRPLHH
                     R"
     gene            743..955
                     /locus_tag="VEV43_10940"
     CDS             743..955
                     /locus_tag="VEV43_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24081.1"
                     /translation="MKRSLKALLVTVAAAVALVVPAAPAQAANCVGVWYDTYGNDHYV
                     VWQCDYCPPLAGLGDGRAWLVYCFEI"
     gene            1081..1812
                     /locus_tag="VEV43_10945"
     CDS             1081..1812
                     /locus_tag="VEV43_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYP24082.1"
                     /translation="MATPEGVTPAAGTTWVKLLRWATIVSIVHVAVITIVVEPIPPLL
                     VFAAIWIGGLVWLGRATKGPAILLLVSFVAFLGLSAPFSIPSLVVPASGGDFIVNLAG
                     ILAALVGIVAAVAVIRGLLDTAPAARSLATVAGAVFVLASAYSVYATATYDNAAAQEG
                     DVELVAKDVKFEQTSLQASGGEISVFVDNEDPTFHTFTIEELDVDLDIPASKSARITF
                     EAEPGEYEFFCEPHKEDMKGTLTVD"
BASE COUNT          267 a          656 c          671 g          282 t
ORIGIN      
        1 tcgaggtcga cgtcacccac ggaggaaaga ccgagacgct gggggccgag gtgctgctgc
       61 tcgcggccgg acgcacgccc gcgacggcgg acgcaggcct cgagcacgcc ggcgtcgagc
      121 gtgacggcgc gtacgtggtg acccgcgacg acgcgctcga gaccaccagt cccggcatct
      181 ccgcggtagg tgacctgctc ggcccccctg cccccgcgcg ggcgaactcc gcgttcgcag
      241 agggcatgct cgtcgccgag cgcatcgccg gcatcgacgg ccccgggatc gactacgcgc
      301 aggtgccgcg cgtcacgcac gggatgatcg agacggcctg cgtcgggctg tccgaggagc
      361 gcgccgcggc cgaagggctc gagccgcgcg ccgcggcgat ccagacgacc ggcgtcgcga
      421 tgggcttgat ctccggcgac cccggcctcg ccaaggtcgt gaccgacggg gacgggaccg
      481 tcgtcggcgt ccacctcgtc ggtccgcacg cgaccgagct gatcccaggc gcggccgccg
      541 tgacgagctt cgaggcgacg gcgtcggagg cagcgtcgat cgtgcacgcg cacccgaccc
      601 tcgccgagtc gatgcaggag ctgtacctcg ccctttcggg ccgcccgctg caccatcgct
      661 gaagggggcg acgggggaca aaaggtgtag aacgtcttcg ggcgggttcc agcgggggag
      721 cccagggaca gggaggccgg acgtgaagcg atctttgaag gcactgctgg tgacggtcgc
      781 ggcggcggtg gcgttggtcg tcccggcggc accggcgcaa gccgcgaact gcgtgggcgt
      841 ctggtacgac acctacggca acgaccatta cgtcgtgtgg cagtgcgact actgcccgcc
      901 cctggccggg ctcggcgacg gccgggcgtg gctcgtgtac tgcttcgaga tctaagcagc
      961 tcgatccgac ggagaagggc ccccctggtg gggcccttct tctttgcccg aaggtcgttg
     1021 ccatcggggc gaggttccgc catcgtcggg gcgtcgaccc tcgaaaggag gcacgaagcc
     1081 gtggcgactc ccgaaggcgt aacgcctgcc gccggcacga cctgggtgaa gctcctgcgg
     1141 tgggcgacga tcgtgtcgat cgtccacgtc gcagtgatca cgatcgtcgt cgagcccatc
     1201 ccgccgctcc tggtcttcgc cgcgatctgg atcggcggcc tcgtctggct gggccgggcg
     1261 acgaaggggc cggcgatcct gctcctggtc tcgttcgtcg ccttcctggg cctgagcgcg
     1321 ccgttctcca tcccgtcgct cgtcgtgccc gcgtccggcg gtgacttcat agtgaacctc
     1381 gccgggatcc tcgccgcgct ggtcgggatc gtcgcggcgg tcgccgtgat ccgcggcttg
     1441 ctcgacaccg ctcccgcagc gcgttcgctc gcgacggtgg ccggggcggt gttcgtcctc
     1501 gcgtccgcgt actcggtcta cgccaccgcg acctacgaca acgcggcggc gcaggagggc
     1561 gacgtcgagc tcgtcgccaa ggacgtcaag ttcgagcaga cgtcgctcca agcctccggc
     1621 ggtgagatct cggtgttcgt cgacaacgag gacccgacgt tccacacgtt cacgatcgag
     1681 gagctcgacg tcgacctcga catccccgcg tccaagtcgg cgcggatcac gttcgaagcc
     1741 gagccgggcg agtacgagtt cttctgcgag ccccacaagg aggacatgaa gggcacgctg
     1801 acggtcgact aggcggccgc ccgcgcgccc ttcctgcggc ccggcagcag caggcagctg
     1861 tcgccgaact cgtgcc
//
LOCUS       DASQDO010000388         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_959008, whole genome shotgun sequence.
ACCESSION   DASQDO010000388 DASQDO010000000
VERSION     DASQDO010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959008"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VEV43_10950"
     CDS             <1..490
                     /locus_tag="VEV43_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24083.1"
                     /translation="GAGAGADAFPQAADGGGSIAWTVVAALFPVVAGAGVVLGGRAAR
                     TDASELVPEAVLATWLLLGTTVAVGVLPGAVGDAGVLGDSAGTIALQLIALAAGAAAG
                     VAARRTRTSVEPPYRPIALAGPAPIPDLPRAAAWSVLALALATAVATGWLTVAGLRVG
                     FL"
     gene            517..>2586
                     /locus_tag="VEV43_10955"
     CDS             517..>2586
                     /locus_tag="VEV43_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYP24084.1"
                     /translation="MRRRGGRAARRAAAGRTTGQRLLLRYGWILPLAAVFAGAGILVL
                     TYAFASIPLPEDVDYLNSSAEVFDRHGRVIGTFTDAERRFLVDTDELLEEKPFIGEAV
                     IAAEDRDFYEHNGVSIRGIARAAWENLTGGKILEGGSTITQQYVKNTVLTPERTVTRK
                     LKEAVLAIKLERQYSKDEILGFYLNTIYLGRGAYGIEAAARSYFDKHADELSLREAAY
                     LASIIPSPEALDPTSNPVGARQRRNQVLQEMAGEGFISQRQATKASRGKVKAIKRVSG
                     APKTQTAAYFMEWLRRDALGPELGPQLYTGGLKIHTTLDLEMQRAAEEAVATTLTEPE
                     EPQAALVSMTPGGQVRALVGGRAFQNVKKARGFNYATDVGRQPGSAFKPFTLLAAIEE
                     GISTSSRFAGHSPMTIPDEECYTDGGPWEVDNYGGSSYGSLDLKQATANSVNTVYAQL
                     IAEIGADKVAALLEDMEFGDGEVPAICSLSLGGALDATALEMARAYSAVSAGGVLPEV
                     TPVTYIEDSHGNCLIEFVQDRDRECETEAKDDGERVVDANSAHVLTQALETVVTSGTA
                     ADAVSLPRPAAGKTGTTQDNKDAWFAGYVPQLTTVVWMGYPLENRKGDDDIVPLMSYC
                     SDLDLCRPVKGIEVTGGSFPAQMWNAYMSVATAEMEVVDFPIPTDLPDEVLNTAPPAP
                     PASVAPTD"
BASE COUNT          398 a          895 c          933 g          360 t
ORIGIN      
        1 gggcgccggt gcgggcgcgg acgcgttccc tcaggccgcc gacggaggcg gctcgatcgc
       61 atggacggtc gtcgcggcgc tgttcccggt cgtcgccggg gccggcgtgg tgctgggggg
      121 cagggccgcg cgcacggatg cgtccgagct cgtcccggag gccgtcctgg ccacgtggct
      181 cctgctcggg accacggtcg cggtcggagt gttgccggga gccgtgggtg acgcaggcgt
      241 cctcggcgac tccgcgggga cgatcgcgct ccagctgatc gcgctcgcag caggcgcggc
      301 tgccggtgtc gcggcacgcc ggacccgcac gtccgtcgag cccccctacc gtccgatagc
      361 gctcgccggt cccgcaccga tccccgacct tccacgcgcc gcggcgtggt cggtgctagc
      421 gctcgcgctc gccaccgcgg tcgcgacggg atggctgacc gtcgcggggt tgcgcgtcgg
      481 cttcctctag gcgcggctcg tatctagact gatcgaatga ggaggagggg tgggcgcgcc
      541 gcccgccggg ccgcggccgg tcgcacgaca ggccagcgtc tgctgctgcg ctacgggtgg
      601 atcctgccgc tggccgcggt gttcgccggc gccggaatcc tcgtcctcac ctatgccttc
      661 gcctcgatcc cgctgcccga agacgtcgac tacctgaact cgtcggccga ggtgttcgac
      721 cggcacggca gggtcatcgg gacgttcacc gacgcggagc ggcgcttcct cgtcgacacc
      781 gacgagctgc tcgaggagaa gccgttcatc ggcgaagcgg tcattgccgc ggaggaccgc
      841 gacttctacg agcacaacgg cgtctcgatc cgcggcatcg cgcgcgcggc ctgggagaac
      901 ctcaccggcg gcaagatcct cgagggtggg tcgacgatca cccagcagta cgtgaagaac
      961 accgtgctga cgccggagcg gacggtgacg aggaagctca aggaggcggt gctcgcgatc
     1021 aagctcgagc gccagtactc gaaggacgag atcctcggct tctacctgaa cacgatctac
     1081 ctcggccgcg gcgcgtacgg gatcgaggcc gcggcccgct cgtacttcga caagcacgcc
     1141 gacgagctgt cgctgcgcga agccgcctat ctcgcgtcga tcatcccctc gccggaggcg
     1201 ctcgacccga cgtcgaaccc cgtcggcgcg cgccagcgcc gcaaccaggt ccttcaggag
     1261 atggccggcg aaggcttcat ctcgcagcgt caggcgacga aggcctctcg gggcaaggtc
     1321 aaggcgataa agcgtgtcag cggtgcaccg aagacccaga cggccgcgta cttcatggag
     1381 tggctgcggc gcgacgccct cggcccggag ctcggccccc agctctacac gggcgggttg
     1441 aagatccaca cgacgctcga cctcgagatg cagcgggccg cggaggaggc ggtcgcgacg
     1501 acgctgacgg aaccggagga gccccaggcc gcgctcgtct cgatgacgcc gggcggtcag
     1561 gtgcgcgcgc tggtcggggg ccgcgcgttc cagaacgtga agaaggcccg cgggttcaac
     1621 tacgcgaccg acgtcggccg gcagccgggg tcggcgttca agccgttcac gctgctcgcc
     1681 gcgatcgagg aggggatctc gacctcgtcg cgcttcgccg gccacagccc gatgacgatc
     1741 ccggacgagg agtgctacac ggacgggggg ccgtgggagg tcgacaacta cggcggcagc
     1801 tcctacggga gcctcgatct caagcaggcg acggcgaact cggtcaacac cgtctacgcg
     1861 cagctgatcg cggagatcgg cgcggacaag gtcgcggcgc tcctggagga catggagttc
     1921 ggggacggcg aggtcccggc gatctgcagc ctctcgctcg gcggcgccct cgacgcgacc
     1981 gcgctcgaga tggcgcgcgc gtacagcgcg gtcagcgcgg gcggcgtcct tccggaggtc
     2041 acgcccgtca cgtacatcga ggactcccac ggcaactgcc tgatcgagtt cgtccaggac
     2101 cgagaccgcg agtgcgagac cgaggcgaag gacgacggcg agcgcgtggt cgacgcgaac
     2161 tccgcgcacg tgctcactca ggccctcgag accgtcgtga cgtcggggac ggccgccgac
     2221 gcggtgtcgt tgccgcggcc ggccgccggt aagacgggga ccacgcagga caacaaggac
     2281 gcgtggttcg ccggctacgt cccgcagctc acgaccgtcg tctggatggg ctatccgctc
     2341 gagaaccgaa agggcgacga cgacatcgtc ccgctgatga gctattgctc ggacctcgac
     2401 ctctgccggc cggtgaaagg gatcgaggtg acgggcggct cgttccccgc gcagatgtgg
     2461 aacgcctaca tgtcggtcgc gaccgcggag atggaggtcg tcgacttccc gataccgacc
     2521 gaccttcccg acgaggtcct caacacggct ccgccggcgc cgcccgcgtc ggtcgcgccg
     2581 acggac
//
LOCUS       DASQDO010000389         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1142703, whole genome shotgun sequence.
ACCESSION   DASQDO010000389 DASQDO010000000
VERSION     DASQDO010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142703"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VEV43_10960"
     CDS             <1..1402
                     /locus_tag="VEV43_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24085.1"
                     /translation="PTFSRRRVKVPPRMGARLARQTSATERQVSGGFRQLPIPFGISG
                     LSDHRVSQLQQLIERERLPLIPYRGSAAQGGSTTQIDPATEPLTPGDSFAAALSYGDV
                     TAAGIGTATMVCNGKALAFGHPFSFEGTTFLGANHAEAITIVEDPVFGSYKLANVGPL
                     VGVVDQDRLAAIRADLGEGPVLIPVTSTVTAQNTNQTRDGKTEVVESEVVPFLAFEHL
                     VANIDSVFDQFTGGSATLSWTIQGVGETTGPFTLTRQNMYADEFDVAFGSAFEILGNL
                     FTLLGNDFEEIKFTSVDMDMTVKEDVELYRIHDVLVSLNGGDYLERRRLKARPGDLIG
                     MQVVLTDFEETEELVVDLAIRLPQKLRGESVIEITGGSAGGPGEECFFFGENCESDEE
                     VDSLAALITALEEGPHNNELMARLRGGRKQKVLSQDVQVLEQVVTGFKRVRIRTPRGG
                     GGGGGKGVVSEPKPAG"
     gene            1586..3325
                     /locus_tag="VEV43_10965"
     CDS             1586..3325
                     /locus_tag="VEV43_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYP24086.1"
                     /translation="MRYVAVVDNPSSGAVTLSLSFEVHPQGRPEDAVAFDRWTGSVAG
                     GSTLRIRGQVTTAQWFSERGRFRVAVRSAVEVPDLTFRVTDPPVVAPAFDDVTESVGL
                     ATEHRAEIECDGYSAGAAWGDVEGDGDLDLFLPHQDGPSQLWLNDAGHFRDVAGESGV
                     AAADDDAVAAVFADYDNDSDQDLYVVNDGPNRLYENDGSGNFADVAPAAGVAEPGPGS
                     SASWGDYDGDGLLDLFVVNWARCGRSSDYTYFDDALFHNEGDGTFTDRTELLESTGST
                     TGAGFQGAWFDYDRDGDVDLYLANDFGGPRPEGNYLWRNDGGTFTNVSVASGTALHMN
                     TMGIAVGDYDRDLDLDMALSNIEATALMRNRGDGTFRDVAARAGVGRPNQRVREKAIT
                     WGLAFNDLNLDGWEDLYVVGGSLAQENRPEPQPNATFVNVGGGRFADLSAAGGAADDG
                     VGRGLSLADYDRDGRMDLYVVNQGGRPILYRNVTPLGPRHWLEVDLVGTTSNRDACGA
                     TLVARVGRAKLLRQVFCGSVGLGGGSDPVVHFGLGRRRTVDSLRIEWPSGRVQTLRDV
                     AGDRLIAIAEPRG"
     gene            3322..>3975
                     /locus_tag="VEV43_10970"
     CDS             3322..>3975
                     /locus_tag="VEV43_10970"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYP24087.1"
                     /translation="MRRVALLTGLLVAAAALAPAGAAFEPEDDPWFVESYGQFRPGPP
                     PADDSAQTGAELRELLRLQRDRTPRQVRIARRWGEGPATIPWTRVALETIRSQRAGAF
                     PTRSARALAVLHLGLHDALEAAEDARRTYSRKRPRRLDDRIDQAVRAGGTSYPDERAV
                     TAGAAGALLRYLFPDAPEGRFERLTEQAAQSRLWAGVAYRSDVTEGLALGRRVAAVAI
                     "
BASE COUNT          560 a         1407 c         1451 g          557 t
ORIGIN      
        1 cccgacgttc tcgcgtcgca gggtcaaggt cccgccccgc atgggtgccc gcctcgcccg
       61 ccagacctcg gcgaccgagc gacaggtctc cggcggcttc cgccagctcc cgatcccctt
      121 cggcatctcg ggcctgtccg accaccgtgt gagccagctg cagcagctca tcgagaggga
      181 gcgtctcccg ctgatcccgt accgcggctc ggccgcgcag ggcggctcga cgacgcagat
      241 cgaccccgcc accgagcctc tcaccccggg tgactcgttc gcggcggcgc tgtcctacgg
      301 cgacgtcact gccgcgggga tcggcacggc gaccatggtc tgcaacggca aggcgctcgc
      361 gttcgggcac ccgttctcgt tcgagggcac gacgttcctc ggcgcgaacc acgccgaggc
      421 gatcacgatc gtcgaagacc cggtgttcgg ctcgtacaag ctcgccaacg tgggtcccct
      481 cgtcggcgtc gtcgaccagg accgcctcgc tgcgatccgc gccgacctcg gggagggccc
      541 ggtcctgatc ccggtgacgt cgacggtgac cgcgcagaac acgaaccaga ctcgcgacgg
      601 caagaccgag gtcgtcgagt ccgaggtcgt gccgttcctc gccttcgagc acctcgtcgc
      661 gaacatcgac tcggtgttcg accagttcac cggcggcagc gcgacgctgt cgtggacgat
      721 ccagggcgtc ggcgagacga ccgggccgtt cacgctgaca cgccagaaca tgtacgccga
      781 cgagttcgac gtcgcgttcg gctccgcgtt cgagatcctc ggcaacctct tcacgcttct
      841 cggcaacgac ttcgaggaga tcaagttcac gtcggtcgac atggacatga cggtgaagga
      901 ggacgtcgag ctctaccgga tccacgacgt cctcgtgtcg ctgaacggcg gcgactacct
      961 cgagcgcagg cgcttgaagg cacggcccgg cgacctcatc ggcatgcagg tcgtgctcac
     1021 ggacttcgag gagaccgagg agctcgtcgt cgatctcgcg atcaggctgc cccagaagct
     1081 gcgcggcgag tccgtgatcg agatcacggg cggctcggcc ggaggtcccg gcgaggagtg
     1141 cttcttcttc ggcgagaact gcgagagcga cgaagaggtc gactccctgg ccgcgctgat
     1201 cacggcgcta gaggaggggc cgcacaacaa cgagctgatg gcccgcctgc gcggtggacg
     1261 caagcagaag gtgctctcgc aggacgtcca ggtcctggag caggtcgtca cgggcttcaa
     1321 gcgggtccgc attcgcacgc ctcgcggcgg gggtggtggc ggcggcaagg gcgtcgtctc
     1381 cgagccgaag cccgcggggt agagcctcct tctcgtgatc agtttccttc cgggggggcc
     1441 gcctaggccc ccccggactc gttccggcgc tgtcctggcc gcggccggcc tgctcgcggc
     1501 cggggtcccc gccggggccc aaacggccgg ggtcgccgtc gcgtggagca cgcccgccgg
     1561 caggacggtg ccccaaggcc tcccgttgcg atacgtcgcc gtcgtcgaca acccgtcctc
     1621 gggggcggtc accttgtcgc tctccttcga ggtgcatccg cagggacggc ccgaggacgc
     1681 ggtcgcgttc gaccgctgga cgggctcggt cgccgggggt tcgacgctcc ggatacgcgg
     1741 gcaggtgacg acggcgcaat ggttctccga gcgcggtcgc ttccgcgtcg cagtgcgttc
     1801 cgcggtcgag gtcccggacc tgacgttccg cgtgacggac ccgcccgtcg tagcgccggc
     1861 gttcgacgac gtgaccgagt cggtcgggct cgcgacggag caccgcgccg agatcgaatg
     1921 cgacgggtac tccgcggggg ccgcctgggg cgacgtcgaa ggagacggcg acctcgacct
     1981 cttcctgccg caccaggacg ggccgtcgca gctgtggctg aacgacgccg gccacttccg
     2041 cgacgtggcg ggggagagcg gggtcgcggc cgccgacgac gacgcggtcg cggccgtgtt
     2101 cgccgactac gacaacgact cggaccagga cctgtacgtc gtcaacgacg gcccgaaccg
     2161 cctgtacgag aacgacgggt ccgggaactt cgccgacgtc gcacccgcag ccggggtggc
     2221 ggagccgggg ccggggtcga gcgcgtcgtg gggcgactac gacggcgacg gcctcctcga
     2281 cctgttcgtc gtgaactggg cgcgctgtgg gcgaagctcc gactacacgt acttcgacga
     2341 cgcgctgttc cacaacgagg gtgacgggac gttcaccgac cggacggagc tcctcgagtc
     2401 gaccggctcg acgacggggg cggggttcca gggcgcgtgg ttcgactacg accgcgacgg
     2461 agacgtcgac ctgtacctgg cgaacgactt cggcggcccg cgcccggagg gcaactacct
     2521 gtggcgcaac gacggcggga ccttcacgaa cgtgtcggtc gcgtccggaa ccgcgctcca
     2581 catgaacacg atgggcatcg ccgtcggcga ctacgaccgc gacctcgacc tcgacatggc
     2641 gctgtccaac atcgaggcga cggccctgat gcgcaaccgc ggtgacggga cgttccgcga
     2701 cgtggccgcg cgggccggcg tggggcggcc gaaccagcgg gtgagggaga aggcgatcac
     2761 gtgggggctc gcgttcaacg acctgaacct cgacggctgg gaggacctct acgtggtcgg
     2821 tggctcgctg gcgcaggaga accgtcccga gccgcagccg aacgcgacgt tcgtgaacgt
     2881 gggcggcggg cgcttcgcgg acctcagcgc ggccggcggg gccgcagacg acggcgtggg
     2941 ccgcgggctc tcgctcgcgg actacgaccg cgacggccgc atggacctct acgtcgtcaa
     3001 ccagggaggc cgtccgatcc tgtaccgcaa cgtgacgccg ctcggccccc ggcactggct
     3061 cgaggtcgat ctcgtgggca cgacgagcaa ccgcgacgcg tgcggagcga cgctcgtcgc
     3121 gcgggtcggt cgcgccaagc tgctccggca ggtcttctgc ggttcggtcg ggctcggcgg
     3181 cgggagcgac cccgtcgtcc acttcgggct cggccgccgc cgcacggtcg actcgctgcg
     3241 catcgagtgg ccgtcgggcc gcgtgcagac gttgcgcgac gtggcggggg accgattgat
     3301 cgcgatcgcg gagccgcgcg ggtgaggcgc gtcgctctcc tgacgggcct gctcgtcgcg
     3361 gcggccgcgc ttgctcccgc gggggccgcg ttcgagcccg aggacgaccc ctggttcgtc
     3421 gagagctacg gccagttccg ccccggcccc cctcccgcgg acgactccgc gcagacgggg
     3481 gccgagctgc gcgagctgct gcgtctccag cgcgaccgca cgccccggca ggtccggatc
     3541 gcacggcgct ggggcgaggg cccggccacg atcccgtgga cgcgcgtcgc cctcgagacg
     3601 atccggagcc agcgcgcggg cgctttcccg acgcgctcgg cgagggcgct cgcggtcctg
     3661 cacctcggtc tgcacgacgc gctcgaggcc gcggaggacg cgcggcgcac gtactcccgc
     3721 aagcggccca ggaggctgga cgaccggatc gaccaggcgg tgcgggcggg cgggacctcg
     3781 tatcccgacg agcgggccgt gaccgcgggc gcggcgggcg cgttgctccg ctacctgttc
     3841 cccgacgcgc cggaggggag gttcgagcgg ctcaccgagc aggccgcgca gtcacgcctc
     3901 tgggccggcg tcgcctaccg cagcgacgtc accgaggggc tcgcattggg ccgccgggtc
     3961 gcggcggtcg ccatc
//
LOCUS       DASQDO010000390         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1142746, whole genome shotgun sequence.
ACCESSION   DASQDO010000390 DASQDO010000000
VERSION     DASQDO010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142746"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /gene="hemG"
                     /locus_tag="VEV43_10975"
     CDS             <1..865
                     /gene="hemG"
                     /locus_tag="VEV43_10975"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HYP24088.1"
                     /translation="TRAGDADEMSLAAAAPELDTIARRHRSLVLGLRAWKPERPQTPV
                     FMAPVGGMTRLVQALKTELLHADVRVGSPVSSVEREGAQFRVVSASGELRADAVVLAA
                     PAHAAARQVESLDAGAAGAIASIPYASVAVVALAYPSSAGAPPKNGSGMLVPRAEART
                     IAACTWYSTKWPASSLPDGFILRAVVGRSGRHHALDLDDDDLVARVHDDLAAMLGLTG
                     SPRSHLVTRWENALPQYTVGHLDRVTGAELALAQKGPLFLAGAGYRGSGIPDCIRQAN
                     EAAAAVVNALD"
     gene            complement(862..1716)
                     /locus_tag="VEV43_10980"
     CDS             complement(862..1716)
                     /locus_tag="VEV43_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYP24089.1"
                     /translation="MTAVPYVDAAELRRLVPMEAAIDALERMFTASDVEVPQRQHLPV
                     GPGELLLMPAWGPEGVGVKLVTVNPSNPAAGRALLNGVYVLFAADSLEPVLMIDGAAV
                     TALRTPAVSALATKHLAPPEARRLLVFGDGAQGRGHVEAMRAVREIESVTVVTRVQGD
                     PGDVARADVVCTCTTSATPLFDGSLLRPGTHVNAVGAYKPAARELDDATIRRAAVYVE
                     DRDAALAEAGDVVQPIESGALDPASIRDLRELIAEGRPDADVTVFKSVGLASEDLAVA
                     VAALAALG"
     gene            complement(1713..>2301)
                     /locus_tag="VEV43_10985"
     CDS             complement(1713..>2301)
                     /locus_tag="VEV43_10985"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HYP24090.1"
                     /translation="AAVRFTNVPSFVWDTGVGVETRAGTIACDVSYGGAFYASVAAGD
                     VGLRVAKEDLPRLIEVGREIKQAIEGAHDVVHPDEPELRGVYGVIFFEEAPADGHALG
                     QRNVTIFADGEVDRSPCGSGTSARLALLDASGELRRGDTLRHESIVGSVFTARVSGET
                     DGGVITEVEGSAYRTGAHEFFLDPADPVRTGFLLR"
BASE COUNT          314 a          874 c          800 g          313 t
ORIGIN      
        1 gacgcgcgcg ggcgacgcgg acgagatgag cctggccgcg gcggcgcccg agctcgacac
       61 gatcgcccgt cgccaccgca gcctcgtgct cgggctccgg gcgtggaagc ccgagcggcc
      121 ccagaccccg gtcttcatgg cacctgtcgg agggatgacg cggctcgtgc aggcgctcaa
      181 gaccgagctc ttgcacgcgg acgtccgggt cgggagcccc gtgtccagcg tcgagcgcga
      241 gggcgcgcag ttccgcgtcg tctcggcgtc cggcgagcta agagccgacg cggtcgtcct
      301 cgccgcgccc gcgcatgcag cggcgcgcca ggtcgagtcg ctcgacgcgg gggccgccgg
      361 cgcgatcgct tcgatcccgt acgcgtcggt cgcggtcgtc gcgctcgcgt acccgtcgtc
      421 cgcgggggcg ccgccgaaga acggcagcgg gatgctcgtc ccccgcgccg aggctcgcac
      481 gatcgccgcg tgcacgtggt actcgacgaa gtggcccgcg tcgtcgctcc ccgacgggtt
      541 catcctgcgc gcagtcgtgg gccggagcgg gcgacaccac gcgctcgacc tcgacgacga
      601 cgacctggtg gcgcgggtgc acgacgacct cgccgcgatg ctcggcctca cgggatcgcc
      661 ccgctcccat ctcgtcacgc gctgggagaa cgctctgccg cagtacacgg tcggacacct
      721 cgaccgggtg acgggtgcgg agcttgcgct cgcccagaag gggccgctct tcctcgccgg
      781 tgccggctac cgcggctcgg ggatcccgga ctgcatccgg caggcgaacg aggccgcggc
      841 ggcggtcgtg aacgcgctgg actagccgag cgctgcgagg gccgccaccg cgacggcgag
      901 gtcttccgac gccaggccca ccgacttgaa caccgtgacg tccgcgtccg ggcggccttc
      961 ggcgatcagc tcccgcaggt cccggatcga agccgggtcg agcgcgcccg actcgatcgg
     1021 ctggacgacg tcgccagctt ccgcgagggc cgcatcccgg tcctccacat agaccgcggc
     1081 cctccggatg gtcgcgtcgt cgagctcacg cgccgccggc ttgtacgcgc cgacggcgtt
     1141 gacgtgcgtg cccgggcgca gcaggctccc gtcgaacagg ggcgtcgcgc tcgtcgtgca
     1201 cgtgcagacg acgtccgcgc gggccacgtc gccggggtct ccctggacac gcgtcacgac
     1261 cgtcacggac tcgatctccc tcactgcccg catcgcctcg acatgcccgc gtccctgggc
     1321 tccgtctccg aagacgagga gccggcgggc ctcgggtggc gccaagtgtt tcgtcgcgag
     1381 ggccgacacg gccggcgtcc tcagcgcggt caccgcggcg ccgtcgatca tcaggaccgg
     1441 ctccaaggag tccgccgcga acagcacgta gacgccgttg aggagcgccc ttccggctgc
     1501 cgggttcgac ggattgaccg tcacgagctt cacgcccacg ccctccggcc cccatgccgg
     1561 catcagtagc agctcgcccg gccccaccgg caggtgctgc cgctgcggca cctcgacgtc
     1621 gctcgcggtg aacatccgct ccagcgcgtc gatcgcggcc tccatcggga cgagacgccg
     1681 cagctccgcc gcgtcgacgt acggaacggc cgtcaacgga gcaggaagcc ggtccggacg
     1741 gggtccgcgg gatccaggaa gaactcgtgc gcacccgttc ggtacgcgct gccctcgacc
     1801 tcggtgatca cgccgccgtc ggtctcgcca gacacccgcg cggtgaagac gctgccgacg
     1861 atgctctcgt gacgaagggt gtcgccacgc cgaagctcgc ccgacgcgtc gaggagcgcg
     1921 aggcgcgcgc tcgtcccgct cccgcacgga gagcggtcga cctcaccgtc ggcgaagatc
     1981 gtgacgttgc gctgcccgag cgcgtggccg tccgcaggag cttcttcgaa gaagatcacg
     2041 ccgtagacgc cgcgcagctc gggctcgtcg ggatggacga cgtcgtgcgc gccttcgatc
     2101 gcttgcttga tctcgcgtcc cacctcgatc agccgcggca ggtcctcctt cgcgacgcgc
     2161 agccccacgt cgccggccgc gaccgacgcg tagaacgcgc cgccgtacga cacgtcacat
     2221 gcgatcgtcc cggctctcgt ctcgacgccg acgcccgtgt cccacacgaa cgacgggacg
     2281 ttcgtgaagc ggacggccgc t
//
LOCUS       DASQDO010000391         7141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_102826, whole genome shotgun sequence.
ACCESSION   DASQDO010000391 DASQDO010000000
VERSION     DASQDO010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102826"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VEV43_10990"
     CDS             <1..185
                     /locus_tag="VEV43_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24091.1"
                     /translation="ARRIYRKGDRMPSETRDRAMQFVDLMLERVEITKYPSKDLMDRI
                     ERTLVLFWEEEQAEGR"
     gene            complement(198..578)
                     /locus_tag="VEV43_10995"
     CDS             complement(198..578)
                     /locus_tag="VEV43_10995"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYP24092.1"
                     /translation="MLEEILVSVVDDRPGILAEIGELLGRAGVNIETLAATNIDGKGV
                     IHLVVDDGEDAGEVLASNGFEVAGSREVLTATLEDRPGELGRYCRRLAEAGVAISSAY
                     VAKRAGGESELILAVDDKEAAAGA"
     gene            complement(601..2061)
                     /locus_tag="VEV43_11000"
     CDS             complement(601..2061)
                     /locus_tag="VEV43_11000"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYP24093.1"
                     /translation="MAQTATRPERADHTPAPAPAPRTAGKRIALRVLADAFAVSAALF
                     LATAMRFEMFEGAPAARFDYTSITLVATPVWIVLFWLYGLYEPRQVLGPVNEFKQVFH
                     GVVAGTVAIFIADSVFTLNLARLWALLALACGMVVVGGERLAVRKVLHFLRRRGGDTT
                     RTIVLGANHEARTVARTLQREAWLGYSIVGFVDDSAPAGHELADGHVVLGGTSELKDL
                     VQRHRVRLVLVAATAFDTGRLNRLFWELQDCDVDLQITSGTIDLMASRMIVQSVAGVP
                     LIYVRKTGMDKIQRTLKRSLDVAGAIAGLVLLSPFLAVIALKIRRDSPGGPFFGQIRV
                     GRDGELFKCWKFRTMYEDAEARRAELEHLNEGPGLLFKLADDPRVTKVGKVLRRYSLD
                     ELPQLWNVLRGEMSLVGPRPALPSEVEQYDDWVRNRLKVKPGMTGLWQVSGRTETSFS
                     DYVRYDLFYIQNWSLSLDLWILWRTLRAVSTAEGAH"
     gene            complement(2075..3691)
                     /locus_tag="VEV43_11005"
     CDS             complement(2075..3691)
                     /locus_tag="VEV43_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP24094.1"
                     /translation="MPSRAAVIGLDGAAWHLLDPMIEAGAMPRLGALRDKGAWGTLLS
                     TVPTYTPPAWTSAATGVNPGRHGIYGFIAGNAQSDRQELVHSGMIKAPAIWEIANDQD
                     ARAGIYNLPLTYPPRPLDGWMVSGMMTPGYGEQLKGFASWSGTGTNAQLEQRILEWAP
                     GYVVDVSANYETDWRDDGLARRALASIRQREAVLKGLLELDPPDVTFTVQEAPDRLQH
                     VYYRYMDPRDPLYGSPDGGRIRDAVVECFAAMDRIVGLLEDYAGSVVVCSDHGFTAWE
                     VSVHVNALLEQWGYLKLKGSGKAMQSGLARKMVPLAKRVLPRKLARQAKGRTFAAIDW
                     ERTTAFASPIPQQGVFVNVAGRERFGIVPPEKLDALKDELTQRFLELRDHEGNPVTDH
                     VHRSEDVFHGDALEGAPDVLPVLRDHRYELDDELFHKEPFTDLRHLPRGVHHPDGIVV
                     VAGPGVRPGRIEGSVADVTPTVLYQAGLAVPEGLDGRVLEDAFEAGTLADRPVASTAA
                     LSSSDKDETSPYSEDEEALIEESLRGLGYL"
     gene            complement(3702..4745)
                     /locus_tag="VEV43_11010"
     CDS             complement(3702..4745)
                     /locus_tag="VEV43_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYP24095.1"
                     /translation="MDFPAIHVYDATPDEADAGRHLGRTLAELGIETPFVVASVHGKG
                     LAAELDAGDDAVVVPIAGADQAWALEVGARAQRMGADAVVAIGGGRCLDVGKLAAGRA
                     GVTIVSVPTQLSHDGICSPVAVVPDETGRAESIPAIVPRAVYLSLPTLVGAPPASVAA
                     GIGDLLANPLALRDWGLAVERGIDEADHRAWDMSAESFALVEPYLDADPSQSMGDPAF
                     MRTLADALVLSGMAMIQAGTSRPASGGEHEISHAIDELYGGRALHGAQVAFGCIVSVR
                     LYGEDHGRFRERLRRLGLPQHPRDLGLTLDDAVRVLLHAPETRPGRYTIVEAAGLDEA
                     AARTVIGDIWAES"
     gene            complement(4748..5488)
                     /locus_tag="VEV43_11015"
     CDS             complement(4748..5488)
                     /locus_tag="VEV43_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HYP24096.1"
                     /translation="MRAVVLAAGRGVRMGGGLVPKTLLPMGDEQPLLSYILRGLRSAG
                     ISDLMVVTGHGRSHLEEYVGEHWAGDKPAFVFNARYASWGNFHSVRVALDQSPGQDVL
                     VVNSDVVVAPDVYARVVAGDGDLVLAVQKKMRLDEEDMRVQLDRRDVKAIGKGIPMGL
                     SHGEYAGVSLLRPDAARAYLHAATNNEWRSVTDVYYEDVYAAMIPLVRVTAVTVREGE
                     YAEVDEPSNVPDAIEVIATHQSAWSATA"
     gene            complement(5485..>7141)
                     /locus_tag="VEV43_11020"
     CDS             complement(5485..>7141)
                     /locus_tag="VEV43_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HYP24097.1"
                     /translation="VAAAEHSAAAAEGTLDVAQNALRGPDKVIAKDPDDPEGGGRIRL
                     DRIEAIARTITQVRKEIRSARDELKAVDLDNLPKAGRRAVKDGIDKAHKTTELLADVE
                     DGFAILPAILGAEGRRTYVLGMQNSAELRGTGGALLQFAIFTIDDGKPEFSKKSSTVY
                     DVDKDRQQLSIPLPEDAWYVAGIPDAQRFGNANWSPDWPLSAEITIDYAQASAELNPE
                     AATIPVDDVDGFIAVDPVAMEKLMPGVGPYTAGKREGRTVYVTANRLVDFVLYKAYAK
                     YPNQKYRRARLRDIVDGFYENVFKPKHPTELIDGFGAALAEKHMQIWMTDPAEQAFVE
                     QMNWDGAIRAARGTDYLYVVEQNVGGNKFDYFAENSTSMDVTVEGDDAMVSTETRTRN
                     GVFLPQPRWSAGDSARDGLHWPMVNVYVPLEAELIGAAYEGERESTPPNLVFETDTPT
                     EHVEKGKKVWSATFRIPPGEEAAMRLDYRVPGVVRSREGRRVYRLIVQRQPKVRPEQL
                     TITLTLPEGARRVDAKGWKRKGDVLTWDKPLKTDLELEVSWQE"
BASE COUNT          996 a         2548 c         2477 g         1120 t
ORIGIN      
        1 atgcgcgacg catctacagg aagggggacc gaatgccgtc cgagacccga gaccgagcga
       61 tgcagttcgt cgacctcatg ctcgagcgcg tcgagatcac caagtacccg agcaaggacc
      121 tcatggaccg gatcgagcgc acgctcgtgc tgttctggga agaggagcag gccgaaggcc
      181 gttagcgggg cttcggatca ggcgccggca gcggcctctt tgtcgtcgac ggccaggatc
      241 agctcgctct cgccgcccgc gcgcttcgcg acgtaggccg acgagatagc gacgccggcc
      301 tcggcgaggc gccggcagta gcggccgagc tcgccgggac ggtcctcgag cgtcgcggtg
      361 aggacctcac gcgacccggc gacctcgaac ccgttcgagg ccagcacctc gcccgcgtcc
      421 tcgccgtcgt cgaccacgag gtggatgacg cccttgccgt cgatgttcgt ggcggcgagg
      481 gtctcgatgt tgacgccggc ccggccgagg agctcgccga tctcggcgag tatcccgggc
      541 cgatcgtcga cgacggagac caggatctct tcgagcatga ggtcagcgta tcccgtcgtt
      601 ctagtgcgcg ccttcggccg tcgagaccgc gcgtagcgtc cgccacagga tccacaggtc
      661 cagcgacagc gaccagttct ggatgtagaa gaggtcgtaa cggacgtagt cggagaagct
      721 ggtctcggta cggccgctca cctgccacag ccccgtcatc cccggcttga ccttcagcct
      781 gttgcgcacc cagtcgtcgt actgctcgac ctcgctcggg agcgcggggc gcggccctac
      841 gaggctcatc tcgccgcgca gcacgttcca cagctgcggg agctcgtcca gcgagtagcg
      901 ccgcagcacc ttgccgacct tcgtcacgcg cgggtcgtcg gcgagcttga agaggagccc
      961 cggcccctcg ttgaggtgct cgagctcggc ccggcgcgcc tccgcgtcct cgtacatcgt
     1021 gcggaacttc cagcacttga agagctcgcc gtcgcggccc acgcgtatct gtccgaagaa
     1081 cggcccgccg ggggagtcgc ggcggatctt gagcgcgatc acggcgagga acggcgacag
     1141 gagcacgagc ccggcgatgg cgccggcgac gtccagcgag cgcttgagcg tgcgctggat
     1201 cttgtccatc ccggtcttgc gcacgtagat cagcgggacg ccggcgacgg actgcacgat
     1261 catccgcgac gccatcaggt cgatcgtccc cgaggtgatc tgcaggtcga cgtcgcagtc
     1321 ctgcagctcc cagaacagcc ggttcaagcg ccccgtgtcg aacgcggtcg ccgccacgag
     1381 gaccaggcgc acccggtggc gctgcacgag gtccttcagc tcggacgtgc cgccgagcac
     1441 gacgtggccg tccgcgagct cgtggcccgc gggggccgag tcgtcgacga agccgacgat
     1501 cgagtagccg agccacgcct cgcgctggag cgtgcgcgcg accgtgcgcg cctcgtggtt
     1561 cgcgccgagc acgatcgtgc gcgtcgtgtc gccgccccgg cgcctcagga agtgcaggac
     1621 cttgcggacg gcgagccgct cgccgccgac gacgaccatg ccgcacgcga gcgcgagcag
     1681 cgcccacagc cgcgcgaggt tgagcgtgaa gaccgagtcc gcgatgaaga tcgcgaccgt
     1741 tccggcgacc acgccgtgaa agacctgctt gaactcgttg accgggccga ggacctggcg
     1801 cggctcgtac agcccgtaga gccagaacag cacgatccac accggcgtcg cgacgagtgt
     1861 gatcgacgtg tagtcgaagc gtgccgcggg cgcgccctcg aacatctcga accgcatggc
     1921 cgtcgcgagg aagagcgccg ccgagaccgc gaacgcgtcg gcgagcacgc gcagcgcgat
     1981 ccgcttgccg gccgtcctcg gtgcgggggc cggagcgggc gtgtggtccg cgcgctccgg
     2041 tctcgttgcg gtctgggcca tgcctctccg tcggttagag gtagccgagc ccccgcagcg
     2101 actcctcgat gagcgcttcc tcgtcctccg agtacggcga ggtctcgtcc ttgtccgacg
     2161 atgatagggc cgcggtgctc gcgacgggcc gatccgcgag cgtcccggcc tcgaacgcgt
     2221 cctccaggac ccgcccgtcg aggccctccg gcaccgccag gcccgcctgg tacagcacgg
     2281 tcggcgtcac gtccgccacc gagccctcga tccggcccgg ccgtacgccg ggcccggcga
     2341 cgacgacgat cccgtccggg tggtggacgc cgcgaggcag atgtctcagg tcggtgaacg
     2401 gctccttgtg gaacagctcg tcgtcgagct cgtagcggtg gtcgcgcagc acgggtagca
     2461 cgtcgggcgc gccctcgagg gcgtctccgt ggaagacgtc ctccgagcgg tgcacgtggt
     2521 cggtgacggg gttcccctcg tggtcgcgga gctcgaggaa cctctgcgtc agctcgtcct
     2581 tcagcgcgtc cagcttctcc ggcgggacga tcccgaagcg ctcgcggccc gcgacgttca
     2641 cgaacactcc ctgctggggg atcggcgacg cgaacgcggt cgtgcgctcc cagtcgatcg
     2701 cggcgaacgt gcggcccttc gcctggcggg cgagcttgcg cggcagcacg cgcttcgcca
     2761 gcgggaccat cttgcgcgcg agccccgact gcatcgcctt cccgcttccc ttgagcttca
     2821 ggtagcccca ttgctcgagc agcgcgttca cgtggaccga cacctcccac gcggtgaagc
     2881 cgtggtccga gcacacgacg acgcttccgg cgtagtcctc gaggaggccg acgatgcggt
     2941 ccatcgcggc gaagcactcg acgacggcgt cgcggatgcg gcccccgtcc ggcgacccgt
     3001 acagcgggtc gcgcgggtcc atgtaccggt agtagacgtg ctgcaggcgg tcgggtgcct
     3061 cctggaccgt gaacgtgacg tccggcgggt cgagttcgag gaggcccttc agcaccgcct
     3121 cgcgctggcg gatggaagcg agagccctcc gcgcgagccc gtcgtcgcgc cagtcggtct
     3181 cgtagttcgc cgagacgtcg acgacgtagc ccggggccca ctcgaggatc cgctgttcga
     3241 gctgcgcgtt cgtcccggtc ccgctccagg aggcgaagcc cttcagctgc tcgccgtacc
     3301 cgggcgtcat catcccggag accatccagc cgtcgagcgg ccgcggcggg tacgtcagcg
     3361 gcaggttgta gatcccggcg cgggcgtcct ggtcgttcgc tatctcccag atcgccgggg
     3421 ccttgatcat cccggagtgg acgagctcct ggcggtcgct ctgcgcgttg cccgcgatga
     3481 acccgtagat cccgtggcgg ccggggttga cgccggtcgc ggcggaggtc cacgccggcg
     3541 gcgtgtaggt cgggaccgtc gacaggagcg tcccccacgc ccccttgtcg cgcagcgcgc
     3601 cgaggcgcgg catcgcgccg gcttcgatca tggggtcgag gaggtgccat gcggccccgt
     3661 cgagaccgat gacggctgct cttgacggca tgacgcgcag tctaggactc ggcccagatg
     3721 tcgccgatga ccgtgcgcgc ggcggcctcg tccagccccg cggcctcgac gatcgtgtag
     3781 cggcccggcc gcgtctcggg cgcatggagc agcactcgga ccgcgtcgtc gagggtgaga
     3841 ccgaggtcgc gcgggtgctg cgggagcccg aggcgccgca gcctctcccg gaaccgtccg
     3901 tggtcctcgc cgtagagccg gaccgagacg atgcacccga acgcgacctg cgcgccgtgc
     3961 agcgcccggc ccccgtacag ctcgtcgatc gcgtggctga tctcgtgctc gcccccggaa
     4021 gcgggccgcg acgtccccgc ctggatcatc gccatgcccg acagcacgag cgcgtcggcg
     4081 agcgtccgca tgaaggccgg gtcgcccatg ctctgcgacg gatcggcgtc gaggtacggc
     4141 tcgacgagcg cgaacgactc ggcggacatg tcccaggcgc ggtggtcggc ctcgtcgatg
     4201 ccgcgctcga ccgcgaggcc ccagtcgcgc agtgccagtg ggttcgcgag caggtcgccg
     4261 atcccggccg ccaccgacgc gggcggcgcg ccgacgagcg tcggcaggga gaggtagacc
     4321 gcgcgcggga cgatcgcagg gatgctctcg gcgcgcccgg tctcgtccgg cacgacggcg
     4381 acgggcgagc agatcccgtc gtgcgagagc tgggtcggga cgctcacgat cgtcacgccc
     4441 gcgcgcccgg ctgcgagctt gcccacgtcg aggcaacgcc ctccgccgat ggcgacgacg
     4501 gcgtcggcgc ccatccgctg cgccctcgcg ccgacctcga gcgcccatgc ttggtccgcc
     4561 cccgcgatcg ggaccacgac cgcatcgtca cccgcgtcga gctcggccgc gagccccttg
     4621 ccgtggaccg aggcgacgac gaagggggtc tcgatcccga gctcggcgag cgtccgcccg
     4681 aggtgccggc ctgcgtcggc ctcgtcgggg gtcgcgtcgt agacgtggat cgcgggaaag
     4741 tccactttca ggccgtcgcg ctccaggcgc tctggtgcgt cgcgatgacc tcgatcgcgt
     4801 ccgggacgtt cgacggttcg tcgacctccg cgtactcgcc ttcgcgcacc gtgaccgcgg
     4861 tcacgcgcac gagcgggatc atcgccgcgt agacgtcttc gtagtagacg tcggtgacgc
     4921 tgcgccattc gttgttggtg gcggcgtgga ggtaggcgcg cgccgcgtcc ggacgcagca
     4981 gcgagacccc cgcgtactcg ccgtggctga ggcccatcgg gatgcccttg ccgatcgcct
     5041 tgacgtcgcg gcggtcgagc tggacgcgca tgtcctcttc gtcgagacgc atcttcttct
     5101 ggaccgcgag cacgaggtcg ccgtcgccgg cgacgacccg cgcgtacacg tccggggcga
     5161 cgacgacgtc ggagttcacg acgagcacgt cctggccggg cgactggtcg agcgcgacgc
     5221 gcacggagtg gaagttcccc cacgacgcgt agcgcgcgtt gaacacgaac gccggcttgt
     5281 caccggccca gtgctcgccg acgtattcct cgaggtggct gcggccgtga cccgtcacga
     5341 ccatgaggtc ggagatgccc gcgctgcgca agccccgcag gatgtacgac agcaggggct
     5401 gctcgtcgcc catgggcaac agcgtcttcg gcacgaggcc gccgcccatc cgcacgccgc
     5461 gtcctgcggc gaggactacg gctctcactc ctgccacgac acctccagct cgaggtcggt
     5521 cttcaacggc ttgtcccagg tgaggacgtc gcccttgcgc ttccatccct tcgcgtccac
     5581 gcgacgggcc ccctcgggaa gcgtcaacgt gatcgtcagc tgctccggcc gcaccttcgg
     5641 ctggcgctgc acgatcagcc tgtagaccct acggccctca cgtgaccgca cgacgccggg
     5701 gacccggtag tcgagacgca tggcggcttc ttcacccggc gggatccgga acgtcgccga
     5761 ccataccttc ttgcccttct cgacatgctc ggtcggggtg tcggtctcga agacgaggtt
     5821 gggcggggtc gactcccgct cgccctcgta cgcggccccg atcagctcgg cctccagcgg
     5881 cacgtagacg ttcaccatcg gccagtgcag cccgtcgcgc gccgagtcgc cggcggacca
     5941 ccggggctgg ggcaggaaca cgccgttgcg cgtgcgcgtc tcggtcgaca ccatcgcgtc
     6001 gtcgccctcg accgtcacgt ccatcgaggt cgagttctcc gcgaagtagt cgaacttgtt
     6061 cccgccgacg ttctgctcga cgacgtacag gtagtcggtg cctcgcgcgg cgcggatcgc
     6121 gccgtcccag ttcatctgct cgacgaacgc ctgctcggcg gggtcggtca tccagatctg
     6181 catgtgcttc tcggcgaggg ctgcaccgaa gccgtcgatc agctcggtcg ggtgcttcgg
     6241 cttgaagacg ttctcgtaga agccgtcgac gatgtcgcgc aggcgggccc tccggtactt
     6301 ctggttcggg tacttcgcgt acgccttgta gaggacgaag tcgacgagcc ggttcgccgt
     6361 cacgtagacc gtccggccct cgcgcttgcc cgcggtgtag ggcccgaccc ccggcatcag
     6421 cttctccatc gcgaccgggt ccaccgcgat gaacccgtcg acgtcgtcga ccgggatcgt
     6481 ggcggcctcc gggttcagct ccgcactggc ctgcgcgtag tcgatcgtga tctccgccga
     6541 caggggccag tccggcgacc agttcgcgtt gccgaaccgt tgcgcgtccg ggatccccgc
     6601 gacgtaccag gcgtcttcgg ggagcgggat cgagagctgc tgccggtcct tgtcgacgtc
     6661 gtacaccgtg ctgctcttct tcgagaactc gggcttgccg tcgtcgatcg tgaagatcgc
     6721 gaactggagc agcgcaccgc ccgtgccccg gagctcggcc gagttctgca tcccgagcac
     6781 gtacgtccgg cggccctcgg cgccgaggat cgccggcagg atcgcgaagc cgtcctcgac
     6841 gtcggcgagg agctccgtcg tcttatgcgc cttgtcgatc ccgtccttca cggcgcggcg
     6901 gccggccttg ggaaggttgt cgaggtcgac tgccttcagc tcgtcacgcg cgctgcggat
     6961 ctccttgcgc acctgggtga tcgtgcgcgc tatcgcttcg atgcggtcga gcctgatgcg
     7021 cccgccgcct tcggggtcgt cggggtcctt cgcgatgacc ttgtccggcc cccgcagcgc
     7081 gttctgtgcg acgtcgagag tcccctcggc ggcggcggcg gaatgctccg cggccgcgac
     7141 g
//
LOCUS       DASQDO010000392         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1142809, whole genome shotgun sequence.
ACCESSION   DASQDO010000392 DASQDO010000000
VERSION     DASQDO010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142809"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..713)
                     /locus_tag="VEV43_11025"
     CDS             complement(168..713)
                     /locus_tag="VEV43_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP24098.1"
                     /translation="MAEGAGRPGLRFLLDPPLDEDATRRLVEVWVAVSNAGGAVGFAP
                     PPPEVAYSDVEPVAAVALRSVDEGRDHLVVAFLGDVLVGLAFLEHRPGPLFRHWATVK
                     RLQVHPAEQGRGIGRELLRACSRFGRELGMEALHLTVRGGTGTESFYEAEGYELIATI
                     PQAIRLSRADTRDELYMVKRL"
     gene            complement(683..>1717)
                     /locus_tag="VEV43_11030"
     CDS             complement(683..>1717)
                     /locus_tag="VEV43_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYP24099.1"
                     /translation="YARGAADNKGDLVARLAGLEVYRAVHGELPVHVKFLVEGEEETG
                     SKSFQSIVTRYGDKLKADGCIWEGMGIDHVGRPEIVFGAKGLAYVELVYRGLAEDQHS
                     SLAVVAPSPVWHLVEALSTLRSPDGKVLVDGFYEHVVPPTAEDEEMLRSLPFEEEAER
                     QRLGVDSFVAGDTGIDLVRRYYFEPTCNIAGIVAGFTVPGASKTVLPKEAMAKLDFRL
                     VPDQDPDDVVAKLRRHLDERGFGDIEINRFSNEHPVRSRSDSDIGRAALAGARDVFEA
                     EPAVSPMMIGTGPMHPVASALGIPTVSPAGVCRPDSNIHAPNENVRVDDFLKIVEYTV
                     SWLKALGDQA"
BASE COUNT          276 a          627 c          562 g          252 t
ORIGIN      
        1 gagctggtct ggccggaact cggcgcgacc accggccatc tgcctcagca atccgagagc
       61 ttcatccgag aaagacaggg agcgacctcc gcggagctcg agtgacttgg cgttgactgg
      121 ataccacccg acgctaacag cgcgtgtggc gggcaccata ggctcggcta gaggcgcttg
      181 accatgtaga gctcgtctct cgtgtcggcc cgcgacagcc tgatcgcttg cgggatcgtc
      241 gctatcagct cgtagccctc ggcttcgtag aacgactccg tccccgtgcc cccgcggacc
      301 gtcaggtgca gggcctccat ccccagctcc cgcccgaacc tcgagcacgc acgcaacagc
      361 tcgcgcccga tcccgcggcc ctgctcggcc gggtggacct gcaacctctt gaccgtcgcc
      421 cagtgacgga acaacgggcc cgggcggtgc tccaggaacg cgagcccgac caggacgtca
      481 ccgaggaacg cgacgactag atggtcccgg ccctcgtcca cggaacgcaa cgccactgcc
      541 gccaccggct ctacgtccga gtacgcgacc tccggcggag gcggcgcgaa cccgaccgca
      601 ccgcccgcgt tcgacacggc gacccacacc tcgaccagcc gccgagtcgc gtcctcgtcg
      661 agcggcgggt cgagcaggaa cctcaggcct ggtcgcccag cgccttcagc cacgacaccg
      721 tgtactccac gatcttcagg aagtcgtcga cgcgcacgtt ctcgttcggc gcgtggatgt
      781 tcgagtcggg ccggcacacg cccgcgggcg acaccgtcgg gatcccgagc gcgctcgcaa
      841 ccgggtgcat cggtcccgtc ccgatcatca tgggcgacac cgcgggctcg gcctcgaaga
      901 cgtcccgcgc gccggcgagc gccgcccgcc cgatgtcgga gtcggaacgc gaccgcaccg
      961 ggtgctcgtt cgagaagcgg ttgatctcga tgtcgccgaa gccgcgctcg tcgagatgcc
     1021 ggcggagctt cgcgacgacg tcgtccgggt cctggtcggg gacgagacgg aagtcgagct
     1081 tcgccatcgc ctccttcggc agcacggtct tcgaggcacc cgggaccgtg aagcccgcga
     1141 cgatcccggc gatgttgcac gtcggctcga agtagtagcg ccgcacgagg tcgatcccgg
     1201 tgtcgcctgc aacgaaggag tcgacgccca gccgctggcg ctcggcctcc tcctcgaacg
     1261 ggagggaccg cagcatctct tcgtcctcgg cggtcggcgg gacgacgtgc tcgtagaacc
     1321 cgtcgacgag gaccttgccg tcggggctcc gcagggtcga gagcgcttcg acgagatgcc
     1381 acaccgggct cggcgcgacg acagcgagcg acgagtgctg gtcctcggcg agcccgcggt
     1441 agacgagctc gacgtatgcg agtcccttag cgccgaacac gatctccggg cgaccgacgt
     1501 ggtcgatgcc catgccctcc cagatgcagc cgtccgcctt cagcttgtcg ccgtagcggg
     1561 tgacgatcga ctggaacgac ttcgacccgg tctcctcctc gccctcgacg aggaacttca
     1621 cgtgaactgg aagctcgccg tgcaccgcgc ggtagacctc gagcccggcc aggcgcgcga
     1681 cgaggtcgcc cttgttgtcg gccgcgccgc gcgcgta
//
LOCUS       DASQDO010000393         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_41100, whole genome shotgun sequence.
ACCESSION   DASQDO010000393 DASQDO010000000
VERSION     DASQDO010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41100"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2501)
                     /gene="nuoG"
                     /locus_tag="VEV43_11035"
     CDS             complement(<1..2501)
                     /gene="nuoG"
                     /locus_tag="VEV43_11035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HYP24100.1"
                     /translation="MENVSLTINGNQVEAEKGRLLIDVAEEMGIFVPRFCYHPGMKSV
                     AVCRMCLVKVEGQNKLLPACATPVADGMNVNTVDEEAVDAQEGMLEFLLINHPLDCPI
                     CDRAGECPLQDQTYAHGPGSSRYVEPKRTYEKALEISELVVLDRERCVLCWRCVRFCE
                     EIAGDQFIQLVDRGAGTQILTFSDEPFDSYFSGNTVQICPVGALLSRPYRFVSRPWDL
                     DTSPSVCSYCSVGCPISNEERSDKLVRCQAMPNENVNDFWVCDKGRYGFHYVNSEDRL
                     DTPLVRNSSGEFEPAGWGQALEKVAGAVKDKKVGVIAGAHLTTEDAYAVARLARKELK
                     TKDIDSRVQDDGAPYELALRLHGAAGSSAEMRDLEAARSVVWIGPDPKETLPVLFLRL
                     RKAVLECNTKLTVIGPRRISLDDFAAHSVRTKAGCEADAIRAIGTDTAVANDLGGPMV
                     VCWGPSSPGRDETAIFDAAIELAQAKEAKLLVCPPHAGSQGMIDMGVHPGLEPGYKVV
                     KEPGRGTREMLEAAAAGELDALLIFGADLISDFPDGALARKALETVGFSAVVELFPTE
                     TALLADVVLPSTAYAEREGTLVNMERRVQKLEPLLAANGSSLEPWRICARLAAALGSD
                     WKIGGFEDLWKEIGKASKPHKGLDVGDLTKPVEAPAPQAESPWTTDPSRGTAMTHGPG
                     GRYPKGHRAGAPFHTGQNWPLSWELRAFEAKQRPGIVPGAAAKPGIKEGAERAVHDPR
                     PGEGFRLYTGRFIYDSGQMVSRTRALRSLAKKPFVEMNDRDVKELGLADGDEVVVSGN
                     GADLTLPLRIGDVAEVAVGSPPPIGDAVINDGPGGR"
     gene            complement(2501..>3554)
                     /locus_tag="VEV43_11040"
     CDS             complement(2501..>3554)
                     /locus_tag="VEV43_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYP24101.1"
                     /translation="EGIIIAAWANQAEKAFIYCRGEFALGARRMDQALNEAYDRGFLG
                     KGIFDTKFDLDIVLHRGAGAYICGEESALLDSLEGYRGQPRLRPPFPAVKGLYGQPTV
                     INNTETLSTLPAIVAKGPDWFKQWGSEKSPGTKMLSVSGHVQKPGNYEVPLGTSLTDV
                     LKLAGGLFPGRTLKAVIPGGASAPLLTTTDIPMDFEALKEAGTMLGSGAIVFMDDTTC
                     MVRNALVTTEFFAHESCGKCTPCREGTWWAVKVLERIEHGEGRPEDMDLMLDICNGID
                     GRSFCPLGDAASWALRSNVKLFREEFEEHVAQGGCPFGDDHRQLVGARQSGAGLGEAG
                     IGSQPAAGIDSDGWEK"
BASE COUNT          496 a         1267 c         1221 g          570 t
ORIGIN      
        1 cgcccgccgg ggccgtcgtt gatgacggcg tcgccgatcg gcggcggcga gccgaccgcg
       61 acctcggcca cgtcgccgat ccgcagcggg agcgtcaggt ccgcgccgtt gccggacacg
      121 acgacctcgt caccgtcggc gagccccagc tccttgacgt cgcggtcgtt catctcgacg
      181 aagggcttct tggcgagcga acgaagggcg cgcgtccgcg acaccatctg accggagtcg
      241 tagatgaaac ggccggtgta gaggcggaag ccctcacccg ggcgcgggtc gtgtacggcc
      301 cgctcggccc cctccttgat ccccggcttg gcggccgcgc cgggaacgat ccccgggcgc
      361 tgcttggcct cgaacgcgcg cagctcccac gacagcggcc agttctgccc cgtgtggaac
      421 ggcgccccgg cgcggtggcc cttcgggtag cggccgccgg gaccgtgcgt catcgcggtg
      481 ccccggctcg ggtccgtcgt ccaaggggac tcggcctgcg gtgccggagc ctcgaccggc
      541 ttcgtcaggt ctccgacgtc gaggcccttg tgcggcttgc tggccttgcc gatctccttc
      601 cacaggtcct cgaacccgcc gatcttccag tcggagccga gcgccgcagc gagccgcgcg
      661 cagatgcgcc acggctccag ggagcttccg ttcgccgcga gcaacggctc gagcttctgc
      721 acgcggcgct ccatgttcac gagcgtcccc tcgcgctcgg cgtacgcggt gctcggcaac
      781 accacgtcgg cgagcagcgc ggtctcggta gggaagagct cgaccaccgc ggagaagccg
      841 acggtctcga gggccttgcg cgcgagggcg ccgtcgggga agtccgagat caggtccgca
      901 ccgaagatca gcagggcgtc gagctcgccg gcggccgcgg cctcgagcat ctcgcgcgtg
      961 ccgcggcccg gctccttgac gaccttgtag cccggctcga gcccggggtg gacgcccatg
     1021 tcgatcatcc cctgcgaccc cgcgtgcggc gggcagacga gcagcttcgc ctccttcgcc
     1081 tgcgcgagct ctatcgcggc gtcgaagatc gccgtctcgt cacgccccgg ggacgacggc
     1141 ccccagcaca cgaccatcgg tccgccgagg tcgttggcca ccgctgtgtc ggtgccgatg
     1201 gcgcggatcg cgtcggcctc gcatccggcc ttcgtgcgga cggagtgcgc ggcgaagtcg
     1261 tcgagcgaga tccgccgcgg cccgatcacg gtcagcttcg tgttgcactc gagcactgcc
     1321 ttgcgtagcc ggaggaacag gaccggcagc gtctccttcg ggtcggggcc gatccagacg
     1381 acgctgcgcg cggcctcgag gtcgcgcatc tccgcggacg agcccgcggc tccgtggagt
     1441 cgcagcgcga gctcgtaggg cgcgccgtcg tcctggacgc gcgagtcgat gtccttcgtc
     1501 ttcagctcct tgcgcgcgag ccgcgcgacc gcgtacgcgt cctcggtcgt caggtgcgcg
     1561 ccggcgatga cgccgacctt cttgtccttc accgcgccgg cgaccttctc cagcgcctgc
     1621 ccccatccgg cgggctcgaa ctcgccggac gagttgcgca cgagcggcgt gtcgagacgg
     1681 tcctcggagt tgacgtagtg gaagccgtag cggcccttgt cgcacaccca gaagtcgttg
     1741 acgttctcgt tgggcatcgc ctggcagcgc acgagcttgt ccgaacgctc ctcgttgctg
     1801 atcgggcagc ccaccgagca gtagctgcac acgctcggcg acgtgtcgag gtcccagggc
     1861 cgcgacacga agcggtaggg acgcgagagc agcgcgccga cggggcagat ctggaccgtg
     1921 ttgccggaga aatagctgtc gaacggctcg tcggagaacg tgaggatctg cgtgccggcc
     1981 cccctgtcca cgagctggat gaactggtcg cccgcgatct cctcgcagaa ccgcacgcag
     2041 cgccagcaca gcacgcagcg ttcccggtcg agcacgacga gctcggagat ctcgagcgcc
     2101 ttctcgtagg tccgcttggg ctcgacatac cggctcgagc ccgggccgtg cgcgtaggtc
     2161 tggtcctgca gcgggcactc gccggcacgg tcgcagatcg ggcagtcgag cgggtggttg
     2221 atcaggagga actcgagcat gccctcctgc gcgtcgaccg cctcttcgtc gacggtgttc
     2281 acgttcatgc cgtcggccac cggcgtggcg catgcgggga gcagcttgtt ctgaccctcg
     2341 accttgacga ggcacatgcg gcacaccgcg acggacttca tgccggggtg gtagcagaag
     2401 cgcgggacga agatgcccat ctcctcggcg acgtcgatga gcaggcggcc cttctcggcc
     2461 tcgacctggt tcccgttgat cgtgagcgag acgttctcca tcacttctcc cacccgtcgc
     2521 tgtcgatccc ggccgcgggc tgcgacccga tgccggcctc gccgaggcct gcgccggatt
     2581 ggcgcgcgcc gacgagctgc cgatggtcgt cgccgaaggg acagccgcct tgcgcgacgt
     2641 gctcctcgaa ctcctcgcgg aacagcttca cgttgctgcg cagcgcccac gacgccgcgt
     2701 ccccgagggg gcagaagctc cgcccgtcga tgccgttgca gatgtcgagc atgaggtcca
     2761 tgtcctcggg gcggccctcg ccgtgctcga tgcgttcgag gaccttcacg gcccaccacg
     2821 tgccctcgcg gcacggcgtg cacttgccgc acgactcgtg ggcgaagaac tcggtcgtca
     2881 cgagcgcgtt gcgcaccatg cacgtcgtgt cgtccatgaa cacgatggcg ccggagccga
     2941 gcatcgtccc cgcttccttg agcgcctcga agtccatcgg gatgtcggtc gtcgtgagca
     3001 gcggggcact cgcgccgccg gggatcacgg ccttcaacgt gcgcccgggg aacaggcctc
     3061 cggcgagctt caagacgtcg gtcagagagg tgccgagcgg gacctcgtag ttgcccggct
     3121 tctgcacgtg acccgacacc gagagcatct tcgtgcccgg cgacttctcg ctcccccact
     3181 gcttgaacca gtcggggccc ttggcgacga tcgccggcag ggtcgacagc gtctcggtgt
     3241 tgttgatcac ggtcggctgc ccgtagaggc ccttgaccgc agggaacggg ggcctgagcc
     3301 gcggctggcc acggtagccc tcgagcgagt ccagcagcgc cgactcctcg ccgcagatgt
     3361 acgcgccggc gccgcggtgc agcacgatgt cgaggtcgaa cttggtgtcg aagatcccct
     3421 tgccgaggaa gccccggtcg tacgcctcgt tgagggcctg gtccatccgc cgcgcgccga
     3481 gcgcgaactc gccccggcag tagatgaatg ccttctcggc ctggttcgcc caggcggcga
     3541 tgatgatgcc ctcg
//
LOCUS       DASQDO010000394         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1143049, whole genome shotgun sequence.
ACCESSION   DASQDO010000394 DASQDO010000000
VERSION     DASQDO010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143049"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VEV43_11045"
     CDS             complement(<1..876)
                     /locus_tag="VEV43_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24102.1"
                     /translation="MPPQKPALRPLHALAAAGLAVGLVTSLIGGVGPGVVALWSFTVA
                     AVALAFALPLPWALVSPLYMGIAGWLVDMLPFVILAGWTAVTVRWGITLLRERRLPRG
                     GRWIWLPIFLVVWTGLGVLVITSLDFKHFLLLLGIQVVASAAVLTVVDALPSHEDRVR
                     LASGLVGFVVILSVAVLLQWVGVPIESLQDSSARRRVETAYGVDAFPNNIGMIKYARS
                     VNAGSLELRRAVTGVAEENPALPAFEVFRPKFQAYENSLVVRFEGSARAQEAELAGAG
                     VTLLYDNVGLAPANTV"
     gene            complement(883..2895)
                     /locus_tag="VEV43_11050"
     CDS             complement(883..2895)
                     /locus_tag="VEV43_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24103.1"
                     /translation="MAASTLTERVQPRPEPASGSRLDRWLGHPAAFLATAAVLLAIFG
                     WTFLENPGRPAPADDPAYYTWRTEALIDNDPVTLLEATGPLDMYSGGYRVTTPVIAAY
                     FRELGGAALLTPTVLLAVGLRVVIPLLLAGFAYRQRRDPLIFHSVAFGSASLLLTPPF
                     GGYLDNVLTLFFLGASLFFIEPSRRSWPARLVFAALLVLSGLTHPTTLVIFCVVLGVM
                     AGARWLFRGFDLKSTLRDDGPMLASAFAAAVVTYLIWKVGIWGKSAPLSDAALPPPAD
                     AEFFKTRLGGWLEAMRPILNGPLFLIGAAGLLAAGKRAAEDELTRVSILWLAPLAGIF
                     GFLAGMAYPYYRFFNTTLSWILLVGVGVYFAARWFLAVSERGGVATLALVGIVALAVV
                     VGTNFTAGLEQSHWNDETDAWLKPEQRDTLLPVRALLEKEDPDTPVVFVVDDEAPENV
                     RIYGFAKLAGNVTRYAVPAEMQDRTAYYLGSLGRLVEGRASERPDEDEYYEDLSEATL
                     DDVKDVLERAGENDPYIVVPAAFNQTGVNSHSFSFGYDEGTAAMVLPPNHAIPQPVAV
                     DDPLPPSETEDGSILRVPIMLVVLALLAIPGWLLMKSVFPEAGLSDALGLVPALAAAL
                     LAFSGIVVLAVARAPFGVGLEWAAYVLALVVSGVLFLRGRRPAAAA"
     gene            complement(2909..>3204)
                     /locus_tag="VEV43_11055"
     CDS             complement(2909..>3204)
                     /locus_tag="VEV43_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24104.1"
                     /translation="AAVDGFGIDPNEVERDIDAEFGPITDAVWPTEVFLDDEALEREG
                     VSVNEVASWLGDYRLEDNAQDADLAGRGVFAPTDRVFELAIPARMLPGIECGG"
BASE COUNT          562 a         1111 c         1142 g          389 t
ORIGIN      
        1 caccgtgttc gcgggagcga gccccacgtt gtcgtagagg agcgtgaccc cggccccggc
       61 cagctccgcc tcctgagcgc gcgccgagcc ctcgaagcgg accacgaggc tgttctcgta
      121 ggcctggaac ttcggccgga acacctcgaa tgccggcagg gccgggttct cctccgcgac
      181 accggtgacc gcccgccgca gctccagcga ccccgcgttc accgagcgcg cgtacttgat
      241 catcccgatg ttgttcggga acgcgtcgac cccgtaagcg gtctcgacgc gacggcgcgc
      301 gctcgagtcc tgcaggctct cgatcggcac gccgacccac tggagcagga ccgctaccga
      361 caggatcacg acgaagccga cgaggccgga agcgagccgg acgcggtcct cgtgcgacgg
      421 cagcgcgtcc acgaccgtca gcaccgcggc gctcgccacc acctggatgc cgagcagcaa
      481 caggaagtgc ttgaagtcga gcgacgtgat cacgaggacg ccgaggcccg tccagacgac
      541 gaggaagatc gggagccata tccaccgccc gccgcgcgga aggcggcgct cgcgcaacag
      601 cgtgatcccc caccggacgg tgaccgccgt ccatccggcg aggatcacga acggcagcat
      661 gtcgacgagc catcccgcga tgcccatgta gagcggcgac acgagcgccc acggcaacgg
      721 caacgcgaac gcgagcgcga ccgcggcgac cgtgaacgac cacagggcga cgacacccgg
      781 cccgacgccg ccgatcagcg acgtgacgag ccctacggcg aggcccgcgg cggcgagggc
      841 gtggagcggg cggagcgctg gcttctgggg gggcaccccg gcctacgcgg cggcggccgg
      901 acgccgccct cggaggaaca gcacccccga gacgacgagc gcgaggacgt acgcggccca
      961 ttcgagcccg acgccgaacg gagcccgcgc tacggcaagg acgacgatcc cggagaatgc
     1021 gagcagggcg gcggcaagcg ccggcacgag gcccagcgcg tcgctgagac cggcttcggg
     1081 gaacaccgac ttcatcagca accagccggg gatggcgagg agcgccagga cgacgagcat
     1141 gatcgggacc cgcaggatcg acccgtcctc ggtctccgac ggaggaagcg ggtcgtcgac
     1201 ggccaccggt tgcgggatcg catggttcgg cggaaggacc attgcggcgg tgccctcgtc
     1261 ataaccgaag ctgaacgaat gcgagttgac gccggtctgg ttgaacgccg cgggtacgac
     1321 gatgtagggg tcgttctcgc cggctcgttc caagacgtct ttgacgtcgt cgagcgtcgc
     1381 ctcggacagg tcttcgtagt actcgtcctc gtcgggccgc tccgacgccc ggccctcgac
     1441 gagcctcccg agggagccca ggtagtacgc cgtgcggtcc tgcatctcgg cagggactgc
     1501 gtacctcgtg acgttgcccg cgagcttcgc gaagccgtag atgcgaacgt tctcgggggc
     1561 ctcgtcgtcg accacgaaga ccaccggggt gtcggggtcc tccttctcga gcagagcgcg
     1621 taccggcagc agggtgtcgc gctgctcggg tttgagccac gcgtccgtct cgtcgttcca
     1681 gtgggactgc tcgaggcccg ccgtgaagtt cgtcccgacg acgaccgcca gggcgacgat
     1741 cccgacgagc gccagcgtcg ccactccacc gcgctccgac accgcgagga accagcgcgc
     1801 cgcgaagtag acgccgacgc cgacgagcag gatccacgac agcgtcgtgt tgaagaagcg
     1861 gtagtacgga tacgccatgc cggcgaggaa gccgaagatc ccggcgaggg gcgcgagcca
     1921 caggatcgag acccgcgtga gctcgtcctc ggcggcccgc ttgcccgcgg cgagcagccc
     1981 ggcggctccg atcaggaaca gggggccgtt gaggatcggc cgcatggcct cgagccatcc
     2041 cccgagacgc gtcttgaaga actcggcgtc ggcgggcggc gggagcgcgg cgtcgctaag
     2101 gggagcggac ttgccccaga tgccgacctt ccagatcagg taggtgacga ctgcggccgc
     2161 gaacgcggac gcgagcatcg gcccgtcgtc acgaagcgtc gacttcagat cgaaccctcg
     2221 gaacagccag cgcgctccgg ccatcacgcc gagcacgacg cagaagatca cgagcgtcgt
     2281 cgggtgcgtc aggcccgaca gcacgagcag cgcggcgaac acgaggcgcg cgggccacga
     2341 tcgccgcgac ggctcgatga agaacagcga cgcgccgagg aagaacagcg tcaggacgtt
     2401 gtcgaggtat ccgccgaacg ggggcgtgag cagcaggctc gcggagccga acgcgaccga
     2461 gtggaagatc agcgggtcgc gcctctggcg gtacgcgaag ccggcgagca gcaacgggat
     2521 cacgacccgc agccccaccg cgagcaggac cgtcggcgtc aggagcgcgg cccccccgag
     2581 ctcgcggaaa tacgccgcga tcacgggcgt cgtcacgcgg tacccgcccg agtacatgtc
     2641 gagcggcccg gtcgcctcga gcaacgtgac cgggtcgttg tcgatcaggg cctccgtccg
     2701 ccacgtgtag taggcggggt cgtccgcggg ggccggacga ccgggatttt ccaggaacgt
     2761 ccagccgaag atcgcgagca ggaccgcggc ggtcgcgagg aacgccgcgg ggtggccgag
     2821 ccagcggtcg agccgcgacc cagacgccgg ttcgggccgg ggctggacgc gctcggtcag
     2881 ggtcgaagca gccatggatg gcggcatcct acccgccgca ctcgatgccg gggagcattc
     2941 gcgccgggat cgcgagctcg aacacacggt cggtaggggc gaagactccg cggcccgcca
     3001 ggtcggcgtc ctgcgcgttg tcctcgagcc ggtagtcgcc cagccagctg gcgacctcgt
     3061 tgaccgagac gccttcgcgc tcgagggcct cgtcgtcgag gaagacctcc gtgggccaga
     3121 ccgcgtccgt gatcgggccg aactccgcgt ctatgtctcg ctcgacctcg ttcgggtcga
     3181 tgccgaaccc gtcgacggcg gccg
//
LOCUS       DASQDO010000395         4533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_469998, whole genome shotgun sequence.
ACCESSION   DASQDO010000395 DASQDO010000000
VERSION     DASQDO010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469998"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VEV43_11060"
     CDS             <1..911
                     /locus_tag="VEV43_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HYP24105.1"
                     /translation="SLLVQRLEGIDNRMARSQGAATDLAQGIFEALGDVRRATAVVAD
                     QAKEFTALQDLLRAPPARGGLGEAMLEELLRQVLPARAYSTQHRFRSGVIVDAVVRAG
                     GKLVCIDSKFPLSNFRAICDASSRPARAAAERAFAADVDRHVTAIAQRYVVPDEGTFD
                     FAVMYVPAEGVYAEMLRLSHGGRPLFERALDAKVVPMSPLTLYGYLQTVLMGLNCLQI
                     EENAARILGFCTRLQKDLELFAGEYETMGAHLTNARNRYEDGSRRLARFRESLDRVAD
                     LDDGTAPRPPDLAEPPGPLEVSARGG"
     gene            complement(908..1942)
                     /locus_tag="VEV43_11065"
     CDS             complement(908..1942)
                     /locus_tag="VEV43_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYP24106.1"
                     /translation="MRALTIDGYGFDNVAVRDLAAPEPGPGEVVVAVKAAAFNHLDLW
                     TLRGDLKIEHRFPHALGADGAGVVESVGEGVPAGIRPGDRVLVNPALSCGLCERCRAG
                     EQSECTAFRMLGEHEPGTVAELVKVPAANVFPVPEHLDFAEAAALGVTFITAYRMLFD
                     RSGMRPGEWVLVTGIGGGLALSLFQLARPAAGKLLVTSSSDEKLQRAADLGADGGVNY
                     RDADVGREVRRLTGKRGVDLVADSAGGPSLDGSLRALRKGGRVVIAGATAGAKAEIDV
                     RRVFWNQLSVIGSTMGSVANVSDMLRLVAGSKLRPIVDRVVPLDGAPAALRLLEEGGQ
                     FGKIVVSMEA"
     gene            2294..2488
                     /locus_tag="VEV43_11070"
     CDS             2294..2488
                     /locus_tag="VEV43_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24107.1"
                     /translation="MSETPAEEAAEPRIPDLGDLVVVLLAGTLAGLRDRLAEDGFEPA
                     AELVADLVDIADDYLTRVAA"
     gene            complement(2521..3423)
                     /locus_tag="VEV43_11075"
     CDS             complement(2521..3423)
                     /locus_tag="VEV43_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HYP24108.1"
                     /translation="MTLGLGAGWKVGHWTDPEARTGCTVVLPPPGNVCSCDIRGSSPS
                     SRELALLDVDRRLTEVHGVLLTGGSAFGLAAAEGVVSWLSEQGIGYETPVARVPIVPA
                     AVVFDLGAGRPDVRPGPAQGRSACESAIEGPPATGLVGAGTGSTVGKWAGREHGVPGG
                     FGFGEASDAGLRAAAVAVVNAVGDVLAGDGSVLAGTTAPRERRTPPAEESSPLPANTV
                     LALVAVEGTFEKREVRWLASRGSDGITIAVRPAHTRYDGDVVFALAAPSARKSEPADL
                     DVLGPLATAAVAAAVRDAVVRQGP"
     gene            complement(3588..3866)
                     /locus_tag="VEV43_11080"
     CDS             complement(3588..3866)
                     /locus_tag="VEV43_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24109.1"
                     /translation="MNDLVLTPDTVIARWSNASLVVHAAGHEGGVTDVGIHRLDANGG
                     SPEFRVTARLAPFAGLFPYGITSVFALPSRPDEIVVVTRDGTERVPVT"
     gene            4006..4524
                     /locus_tag="VEV43_11085"
     CDS             4006..4524
                     /locus_tag="VEV43_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP24110.1"
                     /translation="MRRRVLAAAAALALLGACGGGHDHDGEAASPDDGHAHSHEHSHG
                     GGDEAPFAFGAPGNPSAATRTIEVVAEDPYRFSPRDLEVEPGETVTFVVTNEGRHEHE
                     FVLGDEEYQASHAQEMAAGGMHHEGNAVTVAPGATEELTWTFPNGGDVLYGCHVAGHY
                     DGGMVGAIRVSA"
BASE COUNT          664 a         1666 c         1613 g          590 t
ORIGIN      
        1 cgtcgttgct cgtccagcgg ctcgaaggga tcgacaaccg aatggcccgc tcccagggcg
       61 cggcgaccga cctcgcgcag gggatcttcg aggcgctcgg ggacgtgcgc cgagcgactg
      121 ccgtcgtcgc cgaccaagcc aaggaattca ccgctctcca agacctgctg cgcgctccgc
      181 cggcacgggg tgggttgggg gaggcgatgc tcgaggagct gttgcgacag gtcctcccgg
      241 cgcgcgcgta ctcgacgcag caccgcttcc ggagcggcgt catcgtcgac gcggtcgtgc
      301 gggccggggg caagctcgtg tgcatcgact cgaagttccc gctgtcgaac ttccgggcga
      361 tctgcgacgc gtcgtcccgg cccgcccggg ccgcggccga gcgcgccttc gcagccgacg
      421 tcgaccgtca cgtcacggcg atcgcgcagc gatacgtcgt gcccgacgaa gggacgttcg
      481 acttcgcggt gatgtacgtg cccgccgagg gcgtctacgc cgagatgctg cggctgtcgc
      541 acgggggccg gccgctgttc gagcgcgctc tcgacgccaa ggtcgtgccg atgagcccgc
      601 tgacgctcta cggctacctg cagacggtgc tgatggggct gaactgtctg cagatcgagg
      661 agaacgccgc tcggatcctc ggcttctgca cgcgcctgca gaaagacctg gagctgttcg
      721 cgggcgagta cgagacgatg ggcgcgcacc tgacgaacgc gcgcaaccgc tacgaagacg
      781 gctcccggcg cctggcgcgc ttccgcgagt ccctggaccg agtagccgac ctggacgacg
      841 ggaccgctcc gcgtccgccg gatctagccg agccacccgg cccgctcgag gtcagcgccc
      901 ggggcggcta ggcctccatc gagacgacga tcttcccgaa ctgcccgcct tcttcgagga
      961 gccgcagagc cgccggggcg ccgtcgagcg gcacgacgcg gtcgacgatc ggccgaagct
     1021 tcgatcccgc gacgagccgg agcatgtccg acacgttcgc cacggagccc atcgtcgacc
     1081 cgatcaccga cagctggttc cagaacaccc ggcgcacgtc gatctcggcc ttcgcgccgg
     1141 ccgtcgcccc tgcgatcacg acgcggcccc ccttgcgcag cgcccgtaac gacccgtcta
     1201 gcgacggccc gcccgccgag tccgcgacga ggtcgacacc tcgcttcccc gtgagccggc
     1261 ggacctcccg gccgacgtcg gcgtcgcgat agttcacgcc tccgtcggcg ccgaggtctg
     1321 ccgcgcgctg cagcttctcg tccgaggacg acgtgacgag gagcttcccg gcggccggcc
     1381 gtgccagctg gaacagcgac agcgcgaggc cgcctccgat gcccgtgacg agcacccact
     1441 cgcccggcct catcccgctg cggtcgaaga gcatcctgta cgcggtgatg aacgtcaccc
     1501 ccagggccgc agcctcggcg aagtccaggt gctcggggac cgggaagacg ttcgccgcgg
     1561 ggaccttgac gagctcggcg acggtcccgg gctcgtgctc gccgagcatg cggaacgcag
     1621 tgcattccga ctgctcgccg gcgcggcaac gctcgcacag cccgcaggac agcgcggggt
     1681 tgacgaggac gcggtcgccc ggacggatcc cggccgggac gccctcgccg actgactcga
     1741 ctaccccggc cccgtcggcg ccgagggcat gtgggaagcg atgctcgatc ttgaggtcgc
     1801 cccggagcgt ccagaggtcc agatggttga aggccgcggc cttgacggcg acgacgacct
     1861 cgccgggccc cggctcgggc gcggcaaggt cccggaccgc gacgttgtcg aagccgtaac
     1921 cgtcgatcgt caacgccctc aagcgtccag ctcctctccc tcgactgcgg ccccggacgc
     1981 tttccccggc cccgtcaccg cgcccggccg ctctcgctcg ttcgtcacgg gcgtctggat
     2041 aacccacccg gccctttctg tcacaccccc tccctaggct gaccccagcc gcactcccgg
     2101 gccaggaggt gagcgttatg gctgctccga agcccgacag caaacccgcc gcaccacggc
     2161 gcgccccgcg ctgtcgtgcc tgcggcgacg agatcgaagt accggacggc tggagtcacg
     2221 gccccgccgt ccgccggcac tactggtccc gtcaccgtga cgtgatgacg cggaccgcaa
     2281 agaaggcgac gtcgtgagcg agaccccggc cgaagaggcc gccgagccac gcatccccga
     2341 cctgggggac ctcgtcgtgg tgctgctcgc agggacgctg gcggggctgc gcgaccgcct
     2401 cgccgaggac ggcttcgagc cggcggccga gctggtcgcc gacctcgtcg acatcgccga
     2461 cgactacctc acgagggtgg cggcatgaag gggctgcggt ggttccgggc cgcacgcagg
     2521 tcaggggcct tgccggacga cggcgtcccg gaccgccgcg gccaccgccg ccgtcgcgag
     2581 cgggccgagg acgtcgaggt ccgcgggctc cgacttgcgc gccgaggggg ccgcgagcgc
     2641 gaacacgacg tcgccgtcat agcgcgtgtg cgccgggcgc accgcgatcg tgatgccgtc
     2701 cgacccccgg ctcgcgagcc agcgaacctc gcgcttctcg aacgtcccct cgacggcgac
     2761 gagggcgagg acggtgttcg ccggcagcgg cgagctttcc tcggcgggag gcgtccgtcg
     2821 ttcgcgcggc gcggtcgtcc ccgcgagcac gctgccgtct ccggcgagca cgtcgcccac
     2881 ggcgttcacg actgcgaccg cggcggcacg gagcccggcg tcggaggcct cgccgaagcc
     2941 gaacccgccg gggacgccgt gctcgcggcc cgcccacttc ccgacggtcg atccggtccc
     3001 ggccccgacg agccccgtcg ccggcggtcc ctcgatcgcg gactcgcacg cgctccgccc
     3061 ctgtgcgggc cccgggcgca cgtccggccg tcctgcgccg aggtcgaaca cgaccgcggc
     3121 ggggacgatc ggcacgcgtg ccaccggggt ctcgtagccg atgccctgtt ccgacagcca
     3181 cgacacgacg ccctcggcgg cggcgagccc gaacgcgctg ccgcccgtga ggagcacacc
     3241 gtggacttcg gtcaggcgcc ggtcgacgtc gagcagcgcg agctcgcggc tgctcgggga
     3301 cgagccccgg atgtcacaag agcagacgtt gcccggcggg ggcaggacga ccgtgcaacc
     3361 ggtacgcgcc tcggggtcgg tccaatggcc gaccttccat ccggctccca gccccagcgt
     3421 catgccgtca tattgcgtca ccagcgtccg gcggaacgca ctgcggcccg gccgcggcga
     3481 gagtcgcgct gccgacgggg gcgaggcccg gggcggggcc caccacgagc ccccggcggg
     3541 ggcgggcgca cacgccgggg ggtcgtgggg gcttgccctg cgggggccta ggtgacgggt
     3601 actcgctcgg tgccgtcgcg cgtcacgacg acgatctcgt cgggacggct cggcagcgcg
     3661 aataccgacg tgatcccgta ggggaacagc ccggcgaagg gagccagccg ggccgtgacc
     3721 ctgaactcgg gcgaaccgcc gttggcgtcg agacggtgga tcccgacgtc ggtcaccccg
     3781 ccctcgtgcc cggccgcgtg cacgacgagg gacgcgttcg accaccgcgc tatgaccgtg
     3841 tccggggtga gaacgaggtc gttcaaacgg tcctccgaac ttcgttttta acgaccgacg
     3901 aaaatcacgt caaaaaaatt gtaaacgaag tcgtcgaatt ccgtaagggg cggtcccgcg
     3961 ggcggggcgc tggtaacttc gaacgggaga tcgggaggcg tgcaaatgcg tagaagggtt
     4021 ctggcggcgg ctgccgcgct ggcgctgctc ggcgcctgcg gcggtggcca cgatcacgac
     4081 ggcgaggcgg cgagcccgga cgacgggcat gcccactcgc acgagcactc gcacggcggc
     4141 ggcgacgagg cccccttcgc attcggggcc cccggcaacc cgtcggcggc gacccggacg
     4201 atcgaggtcg tggccgagga cccgtaccgg ttctcgccgc gagacctcga ggtcgagccc
     4261 ggcgagaccg tcacgttcgt cgtgacgaac gagggccgcc acgagcacga gttcgtgctc
     4321 ggagacgagg agtaccaggc gagccatgcc caggagatgg ctgccggcgg catgcaccac
     4381 gagggcaacg ccgtcaccgt ggcccccggg gcgaccgagg agctgacgtg gacgttcccc
     4441 aacgggggag acgtcttgta cggctgccac gtcgcggggc actacgacgg cgggatggtc
     4501 ggagcgatcc gcgtctcggc ctgacccccc ggc
//
LOCUS       DASQDO010000396         6042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_837334, whole genome shotgun sequence.
ACCESSION   DASQDO010000396 DASQDO010000000
VERSION     DASQDO010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837334"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VEV43_11090"
     CDS             <1..1275
                     /locus_tag="VEV43_11090"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYP24111.1"
                     /translation="RELYERAAEAMPFGVASSFQAGDPYPIYLTGGRGSRVTDVDGHE
                     YVDFHNGFGTMAVGHAHPRVRAAIEAAAANGTHFAATTETAILVAEELKRRFGIDKVR
                     FTNSGTEATMDAVRLGRAAAGKDVLLKIEGSYHGHHDAVMFSVVPTLDSSGPDERPWS
                     VPFSRGIPADTSNDTMVVPFNDAEALEETLRTSGDRIGALIMEPVMMNIGIVMPQPGY
                     LEKVRELCTRYGVVLIFDEVKTGCTIAPGGATGMFGVQPDLVCLAKAIGGGVPVGAFG
                     GRAEIMDEIMNGVAALGTFNGNPLSMAAALATLTEVLDDAAYEHLRSLGTMLAQGCQK
                     ALDEAGIEAVTMDLGCKGSITFRDRPLERYRDFQGVDDSLFEAYWWWLVNRGIYQTPG
                     KEEQWTLSVQHTEADIEQFLETFKGYCEAVAG"
     gene            complement(1288..2109)
                     /locus_tag="VEV43_11095"
     CDS             complement(1288..2109)
                     /locus_tag="VEV43_11095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24112.1"
                     /translation="MSRRSRFLPVYTGLVIAYLFVPIVVMILFGFNDPRGRFNFAWQG
                     FTLQWYRELFDIPELTTALKNSLLVAGLSTLIATVLGTMVALALSRYRMRGRSALNVF
                     VFLPMATPEIVLGVSLLSLFVTLGVARGFVTIVIAHILFSVSYVVVTVRARLAGLDHS
                     LEDAAQDLGATPWTTFWTVTFPLIFPGVLAAGLLAFVLSIDDYVITSFNAGGTVTFPL
                     WVFGASRIGVPPQVNVMGTLIFLIGVVYVIVSVVRGRERTGSVGAGALAAKTAKG"
     gene            complement(2106..2960)
                     /locus_tag="VEV43_11100"
     CDS             complement(2106..2960)
                     /locus_tag="VEV43_11100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24113.1"
                     /translation="MPASLKRRLTPYLLTLPGALWLLAFFLVPMVTMLSVSLQEGSLE
                     EGYRFTGHWQTYTEALGKFDTQLLRSIWYGAIVTVVTLVVSYPMAYWIALRGGRRKNL
                     YLLLILLPFFTPFIIRTLSWKFILADEGIFLGALKDWGVLPDAYQVLATSTAVVAGMI
                     YNFLPFMALPLYVALERLDPAMLDAASDLYSSRRQAFLKVTLPLSMPGVFAGCLLTFI
                     PAVGDFVNATFLGGVNTTMIGNVIQREFLTNNDYPEGAAISFLLMAAILVGVLRYVRK
                     MGAEELTV"
     gene            complement(2988..4217)
                     /locus_tag="VEV43_11105"
     CDS             complement(2988..4217)
                     /locus_tag="VEV43_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYP24114.1"
                     /translation="MTDRERELRRLLASAPRHNVSRRDVLRGAGALSLTAWLAACGVS
                     GTNEDGGGGNGGSEDDGLTTTEQNGQFSFANWPLYIDRAKGRRPTIDDFTKDTGIEVD
                     YKEVIDDNESFFGTIREPLASGQGAGWDMIVVTDWLIAKMARLGYLEELDHSLLPNFE
                     AHAGQVYKDPSYDPGNAHSVPWQSGITGIGYNPRLTGRKIKSFDDLFDPAFEGKVGMF
                     KEMRDTMNLTLLGIGIDPQQATVDDAALAQEKLIEQRDKGIVRNYYGNEYADALVRED
                     LWLTMAWSGDVFQLQFDKPELEFVVPEEGGILWVDNMAIPQGAENPIDAMAFMDYVYD
                     PKVAAQITAWVNYICPVPEAQDILKKADDSYTKSVAKSPLVFPTPEMESLLYHYKDLD
                     EEEEEAWNDLFGEVIQG"
     gene            complement(4214..5284)
                     /locus_tag="VEV43_11110"
     CDS             complement(4214..5284)
                     /locus_tag="VEV43_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP24115.1"
                     /translation="MGEVRLEGVTKTFKDTTAVDDVSITIAPGEFFSLLGPSGCGKTT
                     TLRMIGGFEEPTAGRVYLAGNDVTGVPAYKRDVNTVFQSYALFPHLDVFENVAFGLKR
                     RKTPKSEIAPRVEEMLELVDLAGLGTRRSNQLSGGQQQRVALARALINRPQVLLLDEP
                     LGALDLKLRKGMQLELKRIQGEVGITFVYVTHDQDEAMTMSDRIAVMNAGRMEQVGPP
                     EDVYELPATEFVAGFLGASNLLDGEVEEATGDMAKVRLGTGDVVLLPPERLPGSKQVR
                     VGVRPEKLQIEGQGGNSVRAQITASTYTGVSTSYECTTASGAKLVVYVQNAGGASSLG
                     PGTNVTLSWEPEHTFAVERRRS"
     gene            complement(5285..>6042)
                     /locus_tag="VEV43_11115"
     CDS             complement(5285..>6042)
                     /locus_tag="VEV43_11115"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYP24116.1"
                     /translation="KDAFIVCDDVDLEVAARGAVWAAYLNSGQVCTSAERFYVLPQVY
                     DDFVDAFVAEAQKVVVGDPMEDATDMGPLVRGVQRDRVETQLAGARSAGATVLYGGDR
                     GEFEKGYYLRPAVVVDVDEDMELMRAETFGPVAPIARVADLDEAIARTNASEYGLGAN
                     VYTRRLDYMMKAMEEIEAGTVWFNDPLTDNEAAPFGGMKASGNGRELGPEGLDDFRQS
                     KHVHIDSKMDAKTWWYPYAAYTAYQAEHGTAPG"
BASE COUNT          989 a         2066 c         2002 g          985 t
ORIGIN      
        1 cgcgagctct acgagcgcgc cgccgaggcg atgccgttcg gggtcgcgtc gtccttccag
       61 gccggcgacc cctacccgat ctacctgacg gggggccggg ggagcagggt caccgacgtc
      121 gacggacacg agtacgtcga cttccacaac gggttcggga cgatggcggt cgggcacgcg
      181 catccgcgcg tacgcgcggc gatcgaggcc gcggccgcga acggcacgca cttcgcggcg
      241 acgaccgaga ccgcgatcct cgtcgcggag gagctcaagc gacgcttcgg gatcgacaag
      301 gtccggttca cgaactcggg gaccgaggcg acgatggacg cggtcaggct cggtcgcgcc
      361 gcggccggca aggacgtcct gctgaagatc gagggcagct accacggcca tcacgacgcg
      421 gtgatgttct cggtcgtccc tactctcgac tcgagcgggc cggacgagcg gccctggtcg
      481 gtgcccttct cgcgcgggat ccccgccgac acctcgaacg acacgatggt cgtcccgttc
      541 aacgacgcag aggctttgga ggagacgctg cgcacgagcg gcgaccgcat cggcgccctg
      601 atcatggagc cggtgatgat gaacatcggc atcgtgatgc cgcagcccgg ctacctcgag
      661 aaggtacgcg agctgtgcac gcgttacggc gtcgtgctga tcttcgacga ggtcaagacc
      721 ggctgcacga tcgcgcccgg gggcgcgacc gggatgttcg gcgtccagcc cgacctggtg
      781 tgtctggcga aggcgatcgg cgggggagtg ccggtcggcg cgttcggggg ccgggccgag
      841 atcatggacg agatcatgaa cggcgtcgcc gcgctgggga cgttcaacgg gaacccgctg
      901 tcgatggcgg cggccctcgc gacgctgacc gaggtgctcg acgacgcggc gtacgagcac
      961 ctgcgctcgc tgggcacgat gctcgcgcag gggtgtcaga aggcactcga cgaggccggg
     1021 atcgaggccg tcacgatgga cctcggctgc aagggatcga tcacgttccg ggaccgcccg
     1081 ctcgagcgct accgcgactt ccagggggtc gacgactcgc tgttcgaggc gtactggtgg
     1141 tggctggtga accggggcat ctaccagacg ccgggcaagg aagagcagtg gacgctgtcg
     1201 gtccagcaca ccgaagccga catcgagcag ttcctggaga ccttcaaggg ctactgtgag
     1261 gcggtagcgg gctagcaggt ccccgggcta tcccttcgcc gttttggcag ctagggcccc
     1321 ggcccccacg gaccccgtcc tctcgcggcc ccgcaccacc gacacgatca cgtagaccac
     1381 accgatcagg aagatcagtg tccccatcac gttgacctgc ggcgggacgc cgatgcgcga
     1441 cgccccgaac acccacagcg ggaacgtcac cgtcccgccc gcgttgaagc tcgtgatcac
     1501 gtagtcgtcg atcgacagca cgaacgcgag cagccccgcg gccaggactc ccgggaagat
     1561 cagcgggaac gtcacggtcc agaacgtcgt ccacggcgtc gcgccgaggt cctgtgccgc
     1621 gtcctccagg ctgtggtcga gccccgccaa gcgcgccctc accgtcacga cgacgtagga
     1681 gaccgagaac aggatgtgcg cgatcacgat cgtgacgaac ccccgcgcca ccccgagcgt
     1741 cacgaacagc gacagcagcg agacgccgag cacgatctcc ggggttgcca tcggcaggaa
     1801 gacgaagacg ttcagcgcgc tccgccccct catccgatat cggctgagcg cgagcgcgac
     1861 catcgtcccg agcacggtcg ctatcagggt gctgagaccc gcgaccagca gcgagttctt
     1921 cagcgcggtc gtcagctcgg ggatgtcgaa cagctcccgg taccactgca gcgtgaagcc
     1981 ctgccaggcg aagttgaagc ggccccgcgg gtcgttgaag ccgaacagga tcatcacgac
     2041 gatcggaacg aacaggtacg cgatgacgag cccggtgtag accggcagga agcgcgagcg
     2101 gcgactcata ccgtcagctc ctcggccccc atcttgcgca cgtaccgcag cacgccgacg
     2161 aggatcgccg ccatcagcag gaacgagatc gcggcgcctt cgggatagtc gttgttcgtg
     2221 aggaactcac gctggatgac gttgccgatc atcgtcgtgt tcacgccgcc caggaacgtg
     2281 gcgttgacga agtcgccgac cgcggggatg aacgtcagca ggcagccggc gaagaccccc
     2341 ggcatcgaca gcggcagggt caccttgagg aacgcctgcc gtctgctcga gtacaggtcc
     2401 gaggcggcgt cgagcatcgc ggggtcgaga cgttcgagcg cgacgtacag cggcagcgcc
     2461 atgaaaggca gaaagttgta gatcatcccg gccacgaccg cagtcgacgt cgccagcacc
     2521 tgataggcgt cgggcagcac gccccagtcc ttcagcgcgc cgaggaagat gccctcgtcg
     2581 gccaggatga acttccacga cagcgtgcgg atgatgaacg gcgtgaagaa cgggagcagg
     2641 atcagcagca ggtagaggtt cttgcgcctg ccccctcgca gcgcgatcca gtacgccatc
     2701 ggatagctca cgacgagcgt gacgaccgtg acgatcgcgc cgtaccagat ggacctcagc
     2761 agctgagtgt cgaacttgcc gagggcttcc gtgtacgtct gccagtgccc ggtgaaccgg
     2821 tagccctcct cgagcgatcc ctcctgtagc gacaccgaca gcatcgtcac catcggcacg
     2881 aggaagaacg ccaggagcca cagggccccc ggcagcgtca ggaggtaagg cgtaaggcgg
     2941 cgcttcaagc tagccggcac cggtcgcctc ggcgagcggt ccagggatca cccttggatg
     3001 acctcgccga agaggtcgtt ccacgcctcc tcttcctctt cgtccaggtc cttgtagtgg
     3061 tagagcagcg actccatctc gggcgtcggg aagaccagcg ggctcttcgc gacggacttc
     3121 gtgtaggagt cgtcggcctt cttcaggatg tcctgggcct cggggaccgg gcagatgtag
     3181 ttgacccacg ccgtgatctg cgcggcgacc ttcgggtcgt agacgtagtc catgaacgcc
     3241 atcgcgtcga tcgggttctc ggccccctgc gggatcgcca tgttgtccac ccagaggatc
     3301 ccgccctctt cgggaacgac gaactcgagc tccggcttgt cgaactggag ctggaacacg
     3361 tcacccgacc acgccatcgt cagccacagg tcctcgcgca cgagcgcgtc ggcgtattcg
     3421 ttgccgtagt agttgcggac gatgcccttg tcgcgctgct cgatgagctt ctcctgcgcg
     3481 agagcggcgt cgtcgaccgt cgcttgctga gggtcgatgc cgatccccag cagcgtgagg
     3541 ttcatcgtgt cgcgcatctc cttgaacatc ccgaccttgc cctcgaacgc ggggtcaaag
     3601 aggtcgtcga agctcttgat cttgcggccg gtgagcctcg ggttgtagcc gatgccggtg
     3661 atgcccgact gccacgggac gctgtgcgcg tttccggggt cgtaggacgg gtccttgtag
     3721 acctggcccg cgtgcgcctc gaagttcggg agcagcgaat gatccagctc ctcgaggtag
     3781 ccgagccgcg ccatcttcgc gatcagccag tcggtgacga cgatcatgtc ccagccggcc
     3841 ccctggccgc tggcgagagg ctcccggatc gtcccgaaga agctctcgtt gtcgtcgatg
     3901 acctccttgt agtcgacctc gatcccggtg tccttcgtga agtcgtcgat cgtcggccgc
     3961 cgtcccttcg cgcggtcgat gtagaggggc cagttggcga agctgaactg cccgttctgc
     4021 tcggtcgtcg tgaggccgtc gtcctcggag ccgccgttgc cgcccccgcc gtcttcgttc
     4081 gtaccggaga cgccgcacgc ggccagccac gcggtcaacg acagcgcccc cgcgccgcgc
     4141 aggacgtccc tgcgcgacac gttgtggcgc ggcgcggacg cgagcagtct tctcaactcc
     4201 cgttcacgat ccgtcatgac cgtctcctct cgactgcgaa ggtgtgctcc ggctcccagc
     4261 tcaacgtcac gttcgtcccc ggtcccagcg acgaggcccc gcccgcgttc tgcacgtaca
     4321 cgacgagctt cgcgccggaa gcggtcgtgc actcgtagga cgtgctgacc ccggtatagg
     4381 tcgaggccgt tatctgcgcg cgcacggagt tgcctccctg cccctcgatc tgcagcttct
     4441 cgggccgtac gcccacgcgg acctgcttcg acccgggcag tcgctccgga ggcagcagga
     4501 cgacgtcgcc cgtgccgagc cgcaccttcg ccatgtcgcc ggtcgcctct tcgacctcgc
     4561 cgtcgaggag gttcgacgcg ccgaggaacc ccgcgacgaa ctcggtcgcg gggagctcgt
     4621 acacgtcctc gggggggccg acctgttcca tgcgtccggc gttcatcacc gcgatccgat
     4681 cggacatcgt catggcctcg tcctggtcgt gcgtgacgta gacgaacgtg atcccgacct
     4741 cgccctggat ccgcttgagc tcgagctgca tgcccttgcg gagcttgagg tcgagcgcgc
     4801 ccagcggctc gtccagcagg agcacctggg gacggttgat cagcgcgcgg gcgagcgcta
     4861 cccgctgttg ctgtcctccg gacagctggt tgctccggcg cgtcccgagc cccgccaggt
     4921 cgacgagctc gagcatctcc tccacgcgcg gagcgatctc gctcttcggg gtcttgcggc
     4981 gcttgagccc gaacgcgacg ttctcgaaga cgtcgaggtg cgggaacaac gcgtacgact
     5041 ggaagaccgt gttcacgtcg cgcttgtacg cggggacgcc ggtcacgtcg ttgccggcga
     5101 ggtagacgcg gcccgccgtg ggctcctcga acccgccgat catgcgaagg gtcgtggtct
     5161 tgccgcaccc ggaggggccg aggagcgaga agaactcacc cggcgcgatc gtgatcgaca
     5221 cgtcgtcgac cgcagtcgtg tccttgaaag tcttcgtgac gccctccagc cggacctcgc
     5281 ccatctagcc gggcgccgtc ccgtgctccg cctggtaggc ggtgtaggcc gcgtacggat
     5341 accaccacgt cttcgcgtcc atcttcgagt cgatgtgcac gtgcttcgac tggcggaagt
     5401 cgtcgagacc ctcgggcccc agctcgcgcc cgttgcccga cgccttcatg cccccgaacg
     5461 gcgcggcctc gttgtccgtc agcgggtcgt tgaaccacac ggtcccggcc tcgatctcct
     5521 ccatcgcctt catcatgtag tcgagccgcc tcgtatagac gttggcgccg agcccgtact
     5581 cgctcgcgtt cgtccgggcg atcgcctcgt cgaggtcggc cacgcgtgcg atcggcgcga
     5641 cggggccgaa cgtctcggcg cgcatcagct ccatgtcttc gtcgacgtcg acgactaccg
     5701 cgggtcgcag gtagtagccc ttctcgaact cgccgcggtc cccgccgtag aggacggtgg
     5761 cgcctgcgct gcgcgccccg gcgagctggg tctcgacgcg gtcgcgctgg accccccgca
     5821 cgagcgggcc catgtccgtc gcgtcctcca tgggatcgcc gacgacgacc ttctgcgcct
     5881 ccgctacgaa ggcgtccacg aagtcgtcgt aaacctgtgg gaggacgtag aaacgctcgg
     5941 cggacgtgca cacctggccg ctgttgaggt aggcggccca gaccgcgccg cgcgctgcga
     6001 cctccaggtc gacgtcgtcg cacacgatga acgcgtcctt gc
//
LOCUS       DASQDO010000397         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_164948, whole genome shotgun sequence.
ACCESSION   DASQDO010000397 DASQDO010000000
VERSION     DASQDO010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164948"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="thiL"
                     /locus_tag="VEV43_11120"
     CDS             complement(<1..756)
                     /gene="thiL"
                     /locus_tag="VEV43_11120"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HYP24117.1"
                     /translation="MIDLVASYLPSPPEGELWSGDDAAVVGGTIVTTDVMVEGEDFEL
                     SYCTGFDLGWKVVAVNVSDVAAMGGRPRHAVASLALPASSRVELVDGIARGLAAAAGR
                     WEVALVGGDLSSAPLVMIGVTVLGVPFGRVVTRSGARPGDRICVTGSLGGSWGGLRLL
                     RAGRGDESPGLVERHLRPRARVEEGRALAERGATAMMDVSDGFAVDLTRLMRASGTGC
                     AVDPVAIPLDPGLREDDALRGAILGGEDFELLAT"
     gene            790..1023
                     /locus_tag="VEV43_11125"
     CDS             790..1023
                     /locus_tag="VEV43_11125"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HYP24118.1"
                     /translation="MSAHDAKEAAVAVMNTIELTGVSAESWGDAAREALKEASRTIRG
                     INKLHVLGTSAVVEDGVIAEYHTEVRIFFEIET"
     gene            complement(1020..2597)
                     /gene="pckA"
                     /locus_tag="VEV43_11130"
     CDS             complement(1020..2597)
                     /gene="pckA"
                     /locus_tag="VEV43_11130"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HYP24119.1"
                     /translation="MPSASLAGIERPGRVHLNLSAPALVEMAVSRGEGTLAQSGALVT
                     RTGDRTGRSPKDRFFVSHGASKDKIEWGPVNQPFEPAQFDALQEKVRAHMEGRDLFIV
                     DGFVGADPEHSIRLRVIAEFAWHALFAKQLFRRPEPGELEGFEPEFVVLSAPSFDAVP
                     ERDGTNSNAFIGLDLERKQVLICGTHYAGEIKKSIFTSANYLLPEQGVLPMHCSANLG
                     AENDVALFFGLSGTGKTTLSADPERRLIGDDEHGWSDNGVFNFEGGCYAKCIRLSREN
                     EPQIWGAIRFGSVVENVVVDEATRRVDYDDASITENTRVAYPLEFIPGYVPEGRAGHA
                     ETIVFLTADAFGVLPPISKLSSEAAMYHFLSGFTAKLAGTEAGLGAEPEATFSTCFGA
                     PFLPLRPSVYAGMLGERIQKHGARVFLVNTGWTGGPFGTGKRMSLPATRAMVSAATSG
                     ALDGIETRNHPIFNLDVPVSCPDVPDEVLDPQSTWDDPEAYEEKARELARMFVKNFER
                     FVDSVPAEVVKAGPRAD"
     gene            2720..2959
                     /locus_tag="VEV43_11135"
     CDS             2720..2959
                     /locus_tag="VEV43_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYP24120.1"
                     /translation="MAAVQAYILIQTEVGKAAQVAREVREIEGVDAAEDVTGPYDVIV
                     RASAPNVDDLGKLVVAKIQAVEGITRTLTCPVVHL"
     gene            2970..4163
                     /locus_tag="VEV43_11140"
     CDS             2970..4163
                     /locus_tag="VEV43_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYP24121.1"
                     /translation="MSGPRDRRGFSTRAVHRPALPEQDGVPLAPPLDLSSTYSFEDTD
                     AFAKASEERVGAGYVYTRWANPTVDAFEAAVADLEGAEDAEAFSSGMAAITCTFMALA
                     RPGARIVAARQLYGNSYSILMTRMKDMGVTTDFFDVDDLDGIEAALPGAALLYCETIG
                     NPRIVVADLPRLGALAQAAGVPLVVDNTFASPALCRPVEHGATLVVHSATKFLGGHHD
                     LVGGIACGDPATVDKLRAVARDFGPTLAPFNAWLALRGLSTLPLRVARSSESALAVAQ
                     ALESSPHVERVDYPGLPSDPSYDLCRRLLGGAGGGTIGLVVAGGRARASRFQEALEVV
                     LPAASLGGTHSLIVHAASVTHTQLSPDELRAVGMDEGFCRLSVGLEDAEDLIEDLLGA
                     LAATA"
     gene            complement(4153..>4399)
                     /locus_tag="VEV43_11145"
     CDS             complement(4153..>4399)
                     /locus_tag="VEV43_11145"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYP24122.1"
                     /translation="IELGKGRKIDEIIGSMNMVAEGVKSCKPIFELGQAAGVWMPITE
                     NVVRICYEGSTVEELVDDLLSREIRSEFEGVEDYLKL"
BASE COUNT          684 a         1549 c         1519 g          647 t
ORIGIN      
        1 cgtcgccagc agctcgaagt cctcgccacc caggatcgcg cccctcaggg cgtcgtcttc
       61 ccgcaggccg gggtcgaggg ggatcgccac ggggtcgacg gcgcatccgg tccccgatgc
      121 cctcatcaaa cgcgtgaggt cgacggcgaa cccgtccgac acgtccatca tcgcggtcgc
      181 cccgcgctcc gccagggcgc ggccctcctc gacgcgcgcc cgagggcgca gatgacgctc
      241 gacgagtccc ggcgactcgt cgccgcggcc ggctcgcagc agcctcaagc cgccccacga
      301 ccccccgagc gagcccgtga cgcagatgcg atcgcccggc cgggccccgc tgcgcgtcac
      361 tacccgtccg aacgggacgc cgagcaccgt gacgccgatc atcacgaggg gggccgaact
      421 caggtcgccg ccgacgagcg ccacttccca ccggccggcc gcagccgcca gtccgcgggc
      481 gatcccgtcg accagctcga cccgcgaaga cgcagggaga gcgaggctcg cgaccgcatg
      541 gcgcggccgg ccacccatcg cggcgacgtc ggagacgttg accgccacca ccttccagcc
      601 gaggtcgaac ccggtgcagt aagagagctc gaagtcctcg ccctcgacca tgacgtcggt
      661 ggtgacgatc gtcccgccga ccacggccgc gtcgtccccg gaccagagct ctccttcggg
      721 gggtgagggc aggtacgagg cgacgagatc gatcacgccg gactcgccca actcggatac
      781 cgttcgcgct tgtccgctca cgacgcgaag gaggcggccg tggccgtgat gaacacgatc
      841 gagctgaccg gcgtctcggc cgagagctgg ggtgacgccg cacgcgaggc gctgaaggag
      901 gcgtcccgca cgatccgggg catcaacaag ctgcatgtgc tcgggacttc cgcggtggtc
      961 gaggacggtg tgatcgccga gtaccacacc gaggtgcgca tcttcttcga gatcgagacc
     1021 tagtcggccc tcgggccggc cttcacgacc tccgccggca cggagtcgac gaagcgctcg
     1081 aagttcttca cgaacatccg cgcgagctcg cgcgccttct cctcgtacgc ctcggggtcg
     1141 tcccacgtcg actgcgggtc gagcacctcg tcggggacgt cggggcagct gaccgggacg
     1201 tcgaggttga agatcgggtg gttgcgcgtc tcgatcccgt cgagggctcc cgaggtcgcg
     1261 gcgctcacca tcgcgcgcgt cgcgggaagg ctcatgcgct tgccggttcc gaacgggccc
     1321 ccggtccagc cggtgttgac gaggaagacg cgagccccgt gcttctggat ccgctcgccg
     1381 agcatcccgg cgtacacgct cgggcgcagc ggcaggaagg gtgccccgaa gcacgtcgag
     1441 aacgtcgcct cgggctcggc ccccaacccc gcctcggtcc ccgccagctt cgccgtgaac
     1501 cccgacagga agtggtacat cgccgcctcg gatgagagct tcgagatcgg gggcagcacg
     1561 ccgaaagcgt cggccgtgag gaagacgatc gtctcggcgt gaccggcccg tccctctggg
     1621 acgtagccgg ggatgaactc gagtggatag gcgacgcggg tgttctcggt gatagacgcg
     1681 tcgtcgtagt cgacccggcg cgtggcctcg tccactacga cgttctcgac gacggacccg
     1741 aaccggatcg caccccagat ctgcggctcg ttctcgcgcg agagacggat gcacttcgcg
     1801 tagcagccgc cttcgaagtt gaagacgccg ttgtccgacc agccgtgctc gtcgtcgccg
     1861 atgagccggc gctccgggtc tgccgacagg gtcgtcttgc ccgtccccga cagcccgaag
     1921 aacagcgcga cgtcgttctc ggccccgagg ttcgccgagc agtgcatcgg caggacgccc
     1981 tgctcgggaa ggaggtagtt cgccgacgtg aagatcgact tcttgatctc tcccgcgtag
     2041 tgggtcccgc agatcagcac ctgcttgcgc tcgaggtcga ggccgatgaa cgcgttcgag
     2101 ttcgtcccgt cgcgctcggg gacggcgtcg aagctcggcg cggacaggac cacgaactcg
     2161 ggctcgaacc cctcgagctc gccgggctcg ggccggcgga agagctgctt cgcgaacagc
     2221 gcgtgccacg cgaactcggc gatcacgcgc agccggatcg aatgctctgg gtcggcgcct
     2281 acgaagccgt cgacgatgaa gaggtcgcgg ccttccatgt gcgcgcgcac cttctcctgc
     2341 agcgcgtcga actgggccgg ctcgaagggc tggttcaccg gcccccactc gatcttgtcc
     2401 ttggacgccc cgtgcgagac gaagaagcgg tccttcggtg agcgtccggt ccggtccccg
     2461 gtgcgcgtga cgagggcgcc cgactgggcg agcgtccctt cgccgcggct gacggccatc
     2521 tcgacgagcg cgggcgcgct gaggttcagg tggacgcgtc cggggcgctc gatccccgca
     2581 agcgaggcgg atggcatcaa caagctcctt gaatggtgtg gatggcggca ccagagacta
     2641 ccggaaggcg tcggaggcgg ccctgctaca ctcgcccgca cccacgacca acaccacgta
     2701 ggcgaacgga aggagcggca tggcagccgt gcaggcgtac atcctcatcc agaccgaagt
     2761 cggcaaggcc gcccaggtcg cgcgcgaggt ccgtgagatc gaaggcgtcg acgccgccga
     2821 ggacgtgacc ggtccctacg acgtcatcgt gcgcgccagc gcgccgaacg tcgacgacct
     2881 cggcaagctc gtcgtcgcga agatccaggc cgtcgagggc atcacgcgca cgctgacctg
     2941 ccccgtcgtc cacctctaga acgagccacg tgagcggtcc ccgcgaccgc cgaggtttct
     3001 ccacccgcgc cgtccatcgt cccgcgctcc ccgagcagga cggggtgccg ctcgcgccgc
     3061 cgctcgacct ctcctcgacg tattcgttcg aggacaccga cgcgttcgcg aaggcctcag
     3121 aggagcgcgt cggcgcgggg tacgtctaca cgcgctgggc gaaccccacc gtcgacgcgt
     3181 tcgaggctgc ggtcgcggac ctcgaggggg ccgaggatgc ggaggcgttc tcgagcggga
     3241 tggccgcgat cacgtgcacg ttcatggccc tggcgcggcc gggcgcgcgg atcgtcgcgg
     3301 cccgccagct gtacggcaac agctactcga tcctgatgac gcgcatgaag gacatgggcg
     3361 tcacgacgga cttcttcgac gtggacgacc tcgacgggat cgaggccgcg cttccggggg
     3421 ccgcgctcct ctactgcgag acgatcggga acccgcggat agtcgtcgcg gacctgccga
     3481 ggctcggagc gctggcgcag gccgccggcg tcccgttggt cgtcgacaac acgtttgcga
     3541 gcccggcgct gtgccgcccg gtcgagcacg gcgcgacgct cgtcgtccac tcggcgacga
     3601 agttcctggg ggggcaccac gacctcgtcg ggggcattgc atgcggcgac ccggcgaccg
     3661 tggacaagct gcgagcggtg gcgcgcgact tcggcccgac gctggcgccg ttcaacgcgt
     3721 ggctcgcgct acggggcctg tcgacgctgc cgctgcgggt ggcgcgctcg tcggagtcgg
     3781 cgctggcggt cgcccaagcg ctggagtcga gcccgcacgt cgagcgggtc gactacccgg
     3841 gcctcccgag cgacccttcg tacgacctgt gccgtcgctt gctcggcggg gcgggcgggg
     3901 gaacgatcgg actggtggta gcagggggcc gcgcgagggc gagccggttc caagaggcct
     3961 tggaggtcgt cctgccggcg gcgtcgctgg gagggacgca ctcgctgata gtgcacgcgg
     4021 cgagcgtgac gcacacgcag ctaagcccgg acgagctaag ggcggtaggg atggacgaag
     4081 gcttctgccg cctgtcggta ggcctggaag acgccgagga cctgatcgaa gacctgctgg
     4141 gggccctcgc agctacagct tgaggtagtc ctcgacaccc tcgaactcgc tccggatctc
     4201 tcggctcagc aggtcgtcca ccagctcctc gaccgtcgaa ccttcgtagc agatccgcac
     4261 gacgttctcc gttatcggca tccacacgcc ggcggcctgg cccagctcga agatcggctt
     4321 acagctcttc acaccttcgg ccaccatgtt catcgagccg atgatctcgt cgatcttgcg
     4381 gcccttgccg agctcgatc
//
LOCUS       DASQDO010000398         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1143471, whole genome shotgun sequence.
ACCESSION   DASQDO010000398 DASQDO010000000
VERSION     DASQDO010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143471"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1480)
                     /locus_tag="VEV43_11150"
     CDS             complement(14..1480)
                     /locus_tag="VEV43_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HYP24123.1"
                     /translation="MRKLVKPLAIAVAVLLAAGGYVAFTALGKRDDSYTVTAYFTKAI
                     GLFENSDVTVLGVAVGKVTNVEPVGTRVRVDMEISTDHPVPVNATATIVPISVISDRY
                     VQLEPPYRAGRALPDGAVLDVDRTRIPAELDDVFLQLKKLLEAIEPGAEGEPGALGEL
                     VVQLDRTLSGHEQDLRGTLIQGARLTDTLADAEEDISGLLVNLDSMFTTLATRAGSFG
                     ELNSNFAAVMQALAESRDDLHGTLANLGTMTEEVGDLIRDNRSLLDRDLERVATLTQT
                     VLKNRKSVKESLAWLPVVGEGLMRAYNPNPVDATDVRDNATQRVECEILDTIPDSPIK
                     DVLEDICQEETGEPDETPVAAEPRTLPELKLDCDEGVRKVKRQIKRLGRMAIEDGVKD
                     DILKPLTKKLRQLAKKCRDLGQEIDEDLLDRLRELTGDVDLDDLDDLEDLDLDDLNVD
                     ELTGNAAGGAVVPDAEGDDAAEEASGWIDGLLDFVGLS"
     gene            complement(1489..2469)
                     /locus_tag="VEV43_11155"
     CDS             complement(1489..2469)
                     /locus_tag="VEV43_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYP24124.1"
                     /translation="MSIKNFRERSPFLIGILSILGIAGGTALAFSLDKIPFVKQSYEL
                     TAEFADAAGLNPENQVRVAGIKVGTVESVELDGDKVVVTLEIDNGTEIPKDARAEIKL
                     ATLLGTKFVEIDGVGGDPFLADGDVIPLERTEVPYEIYQAANQGTNVLEGLNGPRLNK
                     MLVQLTRLTEAAEDEVGEALAGLNDLGTGVNAREDELKELLSGAGELTALLADEGDEV
                     VRLIDASNDVLGSLASKREEIQSLLEVTRQMAGEVTGLVRDNRSKIDSIVGKLDRALA
                     VLESNVEHIDMALEWAGPSSRYFGNITRQGRWADIFSCVLVVSDGCENDE"
     gene            complement(2466..>2719)
                     /locus_tag="VEV43_11160"
     CDS             complement(2466..>2719)
                     /locus_tag="VEV43_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24125.1"
                     /translation="SGRFGGLVSRNRDNLDQELSDLNVLLTIVDENLRPLDKVAKNLK
                     EVLLATARSQSYGKWWTLYVVNLCPELPAQECAGLGALP"
BASE COUNT          370 a          923 c          936 g          490 t
ORIGIN      
        1 gacgcgcgcc gcttcacgac agccccacga agtcgagtag cccgtcgatc cagccgctcg
       61 cttcctcggc ggcgtcgtcg ccctcggcgt ccggcacgac cgcaccaccc gccgcgttcc
      121 cggtgagctc gtccacgttc aggtcgtcga ggtcgaggtc ttccaggtcg tcgaggtcgt
      181 cgaggtccac gtcaccggtc agctccctca gccggtcgag gaggtcttcg tcgatctcct
      241 ggccgaggtc gcggcacttc ttggcgagct ggcggagctt cttcgtcagc ggcttcagga
      301 tgtcgtcttt gacgccgtcc tctatcgcca tccggccgag ccgtttgatc tggcgcttga
      361 ccttgcgcac gccctcgtcg cagtcgagct tcagctccgg cagcgtccgg ggctcggcgg
      421 cgacaggggt ctcgtcgggc tcgcccgtct cctcctggca gatgtcctcg agcacgtcct
      481 tgatcgggct gtcggggatc gtgtcgagga tctcgcactc gacacgctgc gtcgcgttgt
      541 cgcgcacgtc ggtcgcgtcc accgggttcg ggttgtaggc acgcatgagg ccttcgccga
      601 cgaccggcag ccaggccagc gactccttca ccgacttccg gttcttcaga acggtctgcg
      661 tgagcgtcgc cacccgctcg aggtcgcggt cgaggagcga gcggttgtcc ctgatgaggt
      721 cgccgacctc ctcggtcatc gtgcctaggt tcgcgagcgt gccgtggagg tcgtcgcggc
      781 tctcggccag cgcctgcatc accgcggcga agttcgagtt gagctcgccg aacgacccgg
      841 ccctcgtcgc gagcgtcgtg aacatgctgt cgaggttgac gaggagaccg gagatgtcct
      901 cctcggcgtc ggcgagcgtg tcggtcagcc gcgcgccctg gatcagcgtc ccgcgcaggt
      961 cctgctcgtg gcccgagagc gtccggtcga gctggacgac gagctcaccc agcgcgcccg
     1021 gctcgccctc ggcccccggc tcgatcgctt cgagcagctt cttgagctgg aggaagacgt
     1081 cgtccagctc cgcgggtatc cgcgtccggt cgacgtcgag cacggcgccg tcggggaggg
     1141 cccgtcccgc acggtacggg ggctcgagct ggacgtagcg gtcggagatg acggagatcg
     1201 ggacgatggt cgcggtcgcg ttcacgggga cggggtggtc cgtcgagatc tccatgtcga
     1261 cgcggaccct cgtcccgacg ggctcgacgt tcgtgacctt gccgacggcg acgccgagca
     1321 cggtgacgtc ggagttctcg aagaggccga tggccttcgt gaagtaggcg gtgacggtgt
     1381 agctgtcgtc gcgcttgccg agggcggtga acgcgacgta accgcccgcg gcgaggagga
     1441 cggcgaccgc gatcgccagc ggcttcacga gcttcctcat cgccgcgttc attcgtcgtt
     1501 ctcgcagccg tcggacacga cgagcacgca ggagaagatg tccgcccagc gtccctgccg
     1561 cgtgatgttc ccgaagtaac gcgacgacgg cccggcccac tcgagggcca tgtcgatgtg
     1621 ctcgacgttg ctctcgagga ccgcgagcgc gcggtcgagc ttgccgacga tcgagtcgat
     1681 cttcgaccgg ttgtcgcgga ctagaccggt gacctcgccg gccatctgcc gggtgacctc
     1741 gagcagcgac tggatctcct cacgcttgct cgcgagcgaa ccgaggacgt cgttcgaggc
     1801 gtcgatcagc cgcaccacct cgtcgccctc gtccgcgagc aacgcggtga gctcgccggc
     1861 cccggacaag agctccttaa gctcgtcctc gcgtgcgttc accccggtgc cgaggtcgtt
     1921 cagccccgcg agggcctcgc cgacctcgtc ctccgcggcc tcggtcagcc tcgtgagctg
     1981 gaccagcatc ttgttgagcc gcgggccgtt cagcccttcg aggacgttcg tgccctggtt
     2041 cgcggcctgg tagatctcgt acgggacctc ggtgcgctcg agcgggatca cgtcgccgtc
     2101 ggccaggaag gggtcgccgc cgacgccgtc gatctcgacg aacttcgtcc cgagcagcgt
     2161 cgcgagcttg atctcggccc gcgcgtcctt cgggatctcg gtcccgttgt cgatctcgag
     2221 cgtcacgacg accttgtcgc cgtcgagctc gaccgactcg acggtgccga ccttgatccc
     2281 ggcgacgcgg acctggttct cggggttcag ccccgcggcg tcggcgaact cggccgtgag
     2341 ctcgtagctc tgcttgacga acgggatctt gtcgagcgag aacgcgagcg cggtccctcc
     2401 ggcgatcccg aggatcgaga ggatgccgat gaggaagggc gagcgctcgc ggaagttctt
     2461 gatgctcatg gcagcgcccc caggcccgcg cactcctgcg cggggagctc gggacagagg
     2521 ttgacgacgt agagcgtcca ccacttgccg taggactggc tgcgcgcggt cgcgagcagg
     2581 acctccttga ggttcttggc gaccttgtcg agcgggcgga ggttctcgtc gacgatcgtc
     2641 agcagcacgt tcaggtcgga gagctcctgg tcgaggttgt cgcggttgcg agagacgagg
     2701 cctccgaagc ggcccgacg
//
LOCUS       DASQDO010000399         5074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1143561, whole genome shotgun sequence.
ACCESSION   DASQDO010000399 DASQDO010000000
VERSION     DASQDO010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143561"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VEV43_11165"
     CDS             <1..558
                     /locus_tag="VEV43_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYP24126.1"
                     /translation="GGQQLGTGWIGAPDTVVTNAHVVAGGGDVEVQDQSGSHTGRVVL
                     FDPGTDVAIVHVTGLTGRPLDLETSELERGTPGATLGFPGSNAPEMDVDPAAVQDSYP
                     ARGRDIYGREDVTRQVYELRASIRQGESGGPFALQNGDVAGVVFAASTTDRNVGYALT
                     GAEISDELEAGAGRTEPVSTGDCAR"
     gene            complement(555..1667)
                     /locus_tag="VEV43_11170"
     CDS             complement(555..1667)
                     /locus_tag="VEV43_11170"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HYP24127.1"
                     /translation="MFNADRLRLARDVRGLKQSELAQRLGVKPSAVSQFEHGRSRPSE
                     STLKALALALEFPIDFFAPTEGHENPPSFFRSLRSTSAGSRKQAEATSDLLHVLTSTI
                     EQYVVFPNAEIPMVPLSDRVTPGEAAREVRRRWGLPGGPVRNMVRLLEQHGVVVARTI
                     RGSDDIDAFSRPFEPRPIVVLAADKKDRARSRFDAAHELGHLVMHPHEEPATKAVEDE
                     AQAFAAEFLMPEADIQRELPVRLDWDRLVKLKFRWGTSIKSLLMRSRTLNVMPESAYV
                     RAMKIYSSRGWNRGEPGDLGTPEIPRLLQRAVELLEGSGIGILRLAEQSRLHIAEVER
                     LLGEAGDVRPRVSLEDSRSSAVTRLHAPSGRARPQP"
     gene            complement(1706..2452)
                     /locus_tag="VEV43_11175"
     CDS             complement(1706..2452)
                     /locus_tag="VEV43_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24128.1"
                     /translation="MDDELRRILGTRFANEVLAVLVGCVRDSYRFRQSVYSEQGGDDS
                     TTFGIGVSRGALNLIERALDDRDDVQAHRPQGSFLIAAENNIQVRCWKIGLTEKDDVE
                     SIRWEGSEAKKAPGRDNDRQLRLELGDDVPPEPGHEKHLPNLVIGHLGNSIDGCCRIV
                     LGTPKDREGWHFHRDIWRIADEDIVRSTVDFGPEPIWTGDEPIGEGLQLPEQDDEDPE
                     VKLRGDEENEPDIALRHDDDGSGSAHPGSA"
     gene            complement(2584..3348)
                     /locus_tag="VEV43_11180"
     CDS             complement(2584..3348)
                     /locus_tag="VEV43_11180"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP24129.1"
                     /translation="MTIERFSLAGKIAVVTGASRGIGRAIALGFAEAGADVAVAARSE
                     DDLETLAKEIDSLGRRALAVRTDVTNREDVEALLDRTVTELGGIDVLVNNAGGSNFTS
                     PLIGLRPAGWDKLLKLNLDSVFHGTQLAAQKMLDHGGGAIIQISSVAGIEGAPGLSPY
                     SAAKGGVRLFSQAVAKELAGSGVRVNVIAPGWIETPLNDWMTKDEGILRSIEAMIPMG
                     RIGRADEIVGAAIYLASDASSFVTGSTVVIDGGQTA"
     gene            3387..4169
                     /locus_tag="VEV43_11185"
     CDS             3387..4169
                     /locus_tag="VEV43_11185"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HYP24130.1"
                     /translation="MERPDLTQVADALVAAGITGPHQSHSRRNNLSKINAMLEGDLDG
                     SFGISGLDRYSVTEVLGFLAELTGCSPDVEDECEFDTLDPQRTVAGIVAAAQRLREEA
                     GRGSALLAVTGHPTGMLEHHIRVVDAYRRAGGKVLQLREDENFSTRSGRHKEVRYNGG
                     VGCLADWGQLKHTHSSEAMEALLESEPWPDVVLGDHGFAGAAIERGIPTIAVMDINDP
                     ALAVASAEGRDVVIIPMDDNRPPRLYEPSWTVFEYVLGGGEL"
     gene            4166..>5074
                     /locus_tag="VEV43_11190"
     CDS             4166..>5074
                     /locus_tag="VEV43_11190"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HYP24131.1"
                     /translation="MSVDLSRAAASALDDCLALKAGESLVVVSDPGTRVIAEVLVSTG
                     RDRGAETVLVEMSERENNGTEPPPMVAAAMAACDVLIAPTTKSVSHTEARRAANAAGA
                     RAATMPGITEEMMARTMSADFASLRPRSREVARLLTEGSEVRITTAVGTDLTLGIEGR
                     TGISDDGDLSEPGAFGNLPPGEAFLAPVEGTTNGRLVIDGTMWPVGRLSEPLVFEIVD
                     GYVEEMSGVHADEVRSVLEKLGREAFAVAELGIGTNDAAQLTGNVLEDEKILGTIHVA
                     IGDNHTFGGTIRVSSHQDGIVLAPTLS"
BASE COUNT          841 a         1624 c         1743 g          866 t
ORIGIN      
        1 ggcgggcagc agctggggac cggatggatc ggcgcgcccg acaccgtcgt gacgaacgcg
       61 cacgtcgtcg cgggcggcgg cgacgtcgag gtccaggacc aatcgggatc gcataccgga
      121 cgggtggtgc tgttcgaccc cggcaccgac gtagcgatcg tccacgtcac cggcctcacc
      181 ggccggccgc tagacctcga gacgtccgag ctcgagcgcg ggacgccggg ggcgacgctc
      241 ggcttcccgg gctcgaacgc gccggagatg gacgtcgacc cggcggccgt gcaggacagc
      301 tacccggcga gaggccgcga catctacggc cgcgaggacg tgacgcgtca ggtctacgag
      361 ctgagggcct cgatccgcca gggcgagtcc ggggggccgt tcgcgctgca gaacggtgac
      421 gtggccgggg tcgtgttcgc ggcgtcgacg acggaccgga acgtgggcta cgcgcttacg
      481 ggggccgaga tatcggacga gctggaagcg ggggccggcc ggaccgagcc ggtctcgacc
      541 ggagattgcg cgcgctaagg ctgtggtctc gccctgcccg acggcgcgtg caatcgggtg
      601 actgccgatg atcgggagtc ctccaatgag actcgcggcc gtacgtcgcc tgcttccccg
      661 agaagacgct cgacttcggc aatgtggagg cgggattgtt ccgccagccg caagattccg
      721 attcctgacc cttccagtag ctcgacagcg cgttgcaaga gcctgggtat ctcgggcgtt
      781 cccagatcgc cgggctcgcc acggttccaa ccacgggacg aataaatctt catcgcccgg
      841 acgtaggcgc tttccggcat tacgttgagc gttcttgatc gcatcaagag tgacttgatc
      901 gaggttcccc aacggaactt caacttcacg aggcgatccc aatctaggcg taccggaagt
      961 tctctttgga tgtcggcctc gggcatcaag aactccgctg caaaagcctg ggcttcgtct
     1021 tcaaccgcct tagttgcggg ctcctcgtgc gggtgcatca ccaaatgacc gagctcgtgc
     1081 gccgcatcaa atcgggatcg cgcacggtct ttcttgtccg cagcgagaac gacgatgggc
     1141 cggggctcga acggccgtga aaaggcatcg atatcgtcac tgccgcgaat cgtcctggcg
     1201 actacgacgc catgttgttc caaaagtcga accatgtttc taacgggtcc accggggagt
     1261 ccccacctcc gccggacctc tcgcgcggcc tccccaggag tcacgcgatc cgaaagcgga
     1321 accatgggga tctcggcgtt gggaaagacg acgtattgct cgatagtgct tgtgagcacg
     1381 tggagcaggt cagacgtcgc ttctgcctgc ttgcggctcc ccgccgaggt cgacctgaga
     1441 cttctaaaga atgaaggcgg gttttcgtgt ccttcggtgg gtgcgaaaaa gtcgatcggg
     1501 aattcaagcg ccaaggccag ggccttgagt gttgattcgc ttggacgtga acgtccgtgc
     1561 tcgaactggg agacggctga cggtttgact cctagtctct gagcgagttc gctttgcttt
     1621 aggcctctaa cgtctcgagc aagtcttaaa cggtcggcgt tgaacatgcg cgcgatatcg
     1681 cgcgcaggca agttgtgact caaagctagg ccgagccagg atgtgcggag cctgaaccgt
     1741 catcgtcatg acgaagagcg atgtcaggct cgttttcctc gtcacctcgg agtttcacct
     1801 cgggatcctc gtcatcctgc tcgggtagct gcaggccttc cccgataggt tcgtcccctg
     1861 tccagatcgg ctccggcccg aagtcgactg tggagcgcac gatgtcctcg tcggcgatcc
     1921 gccaaatgtc acggtggaag tgccatccct cacggtcttt tggcgttccg aggacaattc
     1981 gacagcaccc gtcgattgag tttcctagat ggccgatgac caggttgggc aagtgcttct
     2041 cgtgacctgg ctctggaggg acgtcgtcac ctagctccag gcgaagctga cggtcgttgt
     2101 ctcggccagg agccttcttg gcctccgagc cctcccaccg gatgctctcg acgtcgtcct
     2161 tctccgtgag gccaatcttc cagcaccgca cctggatgtt gttctcggcg gcgatcagaa
     2221 atgagccttg aggtcggtgg gcctggacgt cgtcgcggtc gtccaaggcg cgttcgatga
     2281 ggttcagcgc cccccgcgac acgccgatgc cgaacgtcgt ggagtcgtcc ccgccttgct
     2341 ccgaataaac gctctggcgg aagcgatacg aatcccgaac acaccccacg agaacggcca
     2401 gaacctcgtt cgcaaagcgt gttcctagga ttcgccgaag ttcgtcgtcc atgggggtcc
     2461 ccttctgtag aacacgcgtc gaaaacttaa gtagaacgct accgacggtg tcaaggattt
     2521 aagtcggacg cccggatcgg tctgcccgga ataagtgacc gcaggcgggt atcgcgccta
     2581 ggcctacgcg gtttggcccc cgtcgatcac cacggtcgat cccgtcacga acgacgacgc
     2641 atccgacgcc aggtagatcg cggcccccac gatctcgtcg gcccggccga tcctccccat
     2701 cgggatcatc gcctcgatcg acctgaggat gccttcgtcc ttcgtcatcc agtcgttgag
     2761 cggcgtctcg atccaacccg gggcgatcac gttgacccgg acgcccgacc cggcgagctc
     2821 cttcgccacc gcctggctga acagacgcac gccccccttc gccgcggagt aaggcgacag
     2881 ccccggcgct ccttcgatcc ccgccaccga cgagatctgg atgatcgcgc cgccgccgtg
     2941 atcgagcatc ttctgcgcgg cgagctgcgt cccgtggaag accgagtcga ggttgagctt
     3001 cagcagcttg tcccaccccg ccgggcggag gccgatcagc ggagacgtga agttcgatcc
     3061 gccggcgttg ttcacgagga cgtcgatgcc gccgagctcc gtcaccgtcc ggtcgaggag
     3121 cgcctcgacg tcctcccggt tcgtgacgtc cgtgcggacc gcgagagcgc gccggccgag
     3181 cgagtcgatc tccttcgcga gcgtctcgag gtcgtcctcg ctccgtgcgg ctacggcgac
     3241 gtccgctccg gcctccgcga agccgagggc gatcgcgcgc ccgatcccgc gcgacgcgcc
     3301 ggtgacgacc gcgatcttgc ccgccagtga gaaccgctcg atggtcacgt gatcgcctcc
     3361 cgtcggatgt ttcctaggat gctgccatgg agcgaccgga cctgacgcag gttgccgacg
     3421 cgctggtggc tgcggggatc acgggcccgc accagagcca ctcgcggcgc aacaacctct
     3481 cgaagatcaa cgcgatgctc gaaggcgacc tcgacgggtc gttcgggatc agcggcctcg
     3541 accgctactc ggtcacggag gtgctggggt tcctcgccga gctcaccgga tgctcgcccg
     3601 acgtcgagga cgagtgcgag ttcgacacgc tcgaccccca gcggacggtc gctgggatcg
     3661 tcgcggcggc gcagcgcctg cgcgaggagg ccggccgggg ctccgccctg ctggccgtga
     3721 cggggcaccc gaccggcatg ctcgagcacc acatccgtgt ggtcgacgcc taccggcgcg
     3781 ccgggggcaa ggtgctgcag ctgcgcgagg acgagaactt ctcgacgagg tcggggaggc
     3841 acaaggaggt ccgctacaac ggcggtgtcg ggtgcctcgc cgactggggg cagctcaagc
     3901 acacgcattc gtcggaagcg atggaggcgt tgctcgagtc ggagccgtgg ccggacgtcg
     3961 tgctgggcga ccacgggttc gcgggggccg cgatcgagcg cgggatcccg acgatcgcgg
     4021 tgatggacat caacgacccg gcgctggcgg ttgcgtcggc ggagggccgg gacgtggtga
     4081 tcatcccgat ggacgacaac cggccaccgc gcctctacga accgtcgtgg acggtcttcg
     4141 agtacgtcct ggggggcggc gagctgtgag cgtcgatctg tcacgtgcgg ccgcgagcgc
     4201 gctcgacgac tgtctcgcgc tgaaggccgg cgagtctttg gtggtcgtgt ccgatccggg
     4261 aactcgggtc atcgccgagg tgctcgtctc gacggggcgc gaccgcgggg cggagaccgt
     4321 gctcgtcgag atgtccgagc gcgagaacaa cgggaccgag ccgccgccga tggtcgcggc
     4381 ggcgatggct gcgtgcgacg tgctgatcgc gccgacgacg aagtcggtgt cgcacaccga
     4441 ggctcgccgc gctgcgaacg ccgcgggggc gagggccgcg acgatgccgg ggatcaccga
     4501 ggagatgatg gcgcgcacga tgtcggcgga cttcgcgtcg ctgcggccgc gctcgcgcga
     4561 ggtcgcccgg ctcctcaccg agggctccga ggtccggatc acgactgctg tggggaccga
     4621 cctgacgctg ggtatcgagg gccggacggg gatctcggac gacggcgacc tcagcgagcc
     4681 gggagcgttc gggaacctgc cgccgggtga ggcgttcctc gcgccggtgg aggggacgac
     4741 gaacggacgg ttggtgatcg acgggacgat gtggccggtc ggccgcctgt ccgagccgct
     4801 ggtcttcgag atcgtcgacg ggtacgtcga ggagatgtcg ggcgtacatg cggacgaggt
     4861 gaggtccgtg ctcgagaagc tcggccggga ggcgttcgcg gtggcggagc tggggatcgg
     4921 gacgaacgac gccgcccagc tgaccggcaa cgtcctggag gacgagaaga tcctgggcac
     4981 gatccacgtg gcgatagggg acaaccacac cttcgggggc acgatccgtg tgtcgagcca
     5041 ccaggacggg atagtcctgg cgccgacgct gtcg
//
LOCUS       DASQDO010000400         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_347872, whole genome shotgun sequence.
ACCESSION   DASQDO010000400 DASQDO010000000
VERSION     DASQDO010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347872"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..2334)
                     /locus_tag="VEV43_11195"
     CDS             complement(43..2334)
                     /locus_tag="VEV43_11195"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HYP24132.1"
                     /translation="MSPEPTYVERKIGDEVFRIETGRLAGQAGGAVLASVGETTVFAA
                     GTSSAPRGDADFFPLTVDVEERMYAAGKIPGGFFRREGRASEKAILNARLIDRPLRPA
                     FPDGFRHEVHVVVNVMAVDGQNPVDILSINAASAALALSDIPFEGPVGAARVSHINGS
                     WVANPTWMQQEEATIELVVAGRVNDAGEVDIMMIEAGASDHAFELVEAGAQSPDEELM
                     ADGIEFSKQYIAEAISLQQELVAQAGKPKGTTTGDDPDYPLFVDYSDELFSRVEQLAG
                     DRLREAISITEKAKRRATIADVSAAIGADLAEEFPEHEKEIKAALRSLTKKLVRRRIV
                     DDGVRLDGRSIDQVRPIWGQVGVIPRVHGSGLFTRGETQALTITTLGMQRMEQMIDDL
                     SPEDRKRYMHHYNFPPYSTGEAGFMRGPKRREIGHGALAEKALLPLIPSKDDFPYAIR
                     VVSEIMSSNGSTSMASVCGSSLSLMDAGVPLRGGKHCGGVAMGLIAEGGKFLPLTDIL
                     GDEDAFGDMDFKVAGTEDMVTALQLDTKISGVPADVLREALMAAKKARLHIIGEMNKT
                     MSSPRSELAAHAPRVLALKVPVDKIGEVIGPKGKNINMITTTTGVDVDIDDDGTIRIG
                     STDQESAERAARLIEEIVNPRMPEVGERIMGTVVKTTTFGAFINIAPGRDGLVHISKL
                     GEGRIDRVEDAANVGDQIEVEITDIDRQGRINLTPIAWLERQVEAGKTIEEARAAAVA
                     GGGGGGGGDRGGRDRDRGGRDRD"
BASE COUNT          358 a          904 c          923 g          458 t
ORIGIN      
        1 gtccctcggc acccggtccc ggccgccgcg gtcgcggccg cctcagtcac ggtcgcggcc
       61 acctcggtcc cggtcccggc caccacgatc gccgccgccg ccgccgccgc cgcctgcgac
      121 ggctgcggca cgggcctctt cgatcgtctt gccggcctcg acctgacgct cgagccatgc
      181 gatcggcgtc aggttgatgc ggccctgacg gtcgatgtcg gtgatctcga cctcgatctg
      241 gtcgccgacg ttcgcggcgt cttcgacgcg atcgatacga ccctccccga gcttcgagat
      301 gtgcacgaga ccgtcacggc ccggtgcgat gttgatgaac gcaccgaacg tcgtcgtctt
      361 cacgaccgtg cccatgatgc gctcgccgac ctcgggcatg cgcgggttga cgatctcctc
      421 gatcaggcgg gccgcacgct cggccgactc ctggtcggtc gacccgatgc ggatcgtgcc
      481 gtcgtcgtcg atgtcgacgt cgacgccggt cgtcgtggtg atcatgttga tgttcttgcc
      541 cttggggccg atgacctcgc cgatcttgtc gaccggcacc ttgagcgcca gcacgcgggg
      601 agcgtgtgcg gcgagctcgg agcgcggcga cgacatcgtc ttgttcatct cgccgatgat
      661 gtgaaggcgg gccttcttgg cggccatgag ggcctcgcgc aggacgtctg ccgggacgcc
      721 cgagatcttc gtgtcgagct gcagggcggt gaccatgtcc tcggtcccgg cgaccttgaa
      781 gtccatgtca ccgaacgcgt cctcgtcacc gaggatgtcc gtgagcggga ggaacttccc
      841 tccctcggcg atgaggccca tcgccacgcc gccgcagtgc ttgccgccgc gcaacgggac
      901 gcccgcgtcc atcagcgaca gcgacgagcc gcacacgctg gccatcgacg tcgagccgtt
      961 ggacgacatg atctcggaca cgaccctgat cgcgtacggg aagtcgtcct tgctcgggat
     1021 gagcgggaga agggccttct cggcgagcgc gccgtggccg atctcgcgcc gcttcggccc
     1081 ccgcatgaac ccggcctcac cggtcgagta cggcgggaag ttgtagtggt gcatgtagcg
     1141 cttgcggtcc tcgggcgaca ggtcgtcgat catctgctcc atgcgctgca tgccgagcgt
     1201 ggtgatcgtc agcgcctgcg tctcgccgcg ggtgaacagg ccggagccgt gcacgcgggg
     1261 gatgacgccg acctggcccc agatcgggcg gacctggtcg atcgagcggc cgtcgaggcg
     1321 gacgccgtcg tcgacgatgc gcctgcgcac gagcttcttc gtcagcgagc ggagggccgc
     1381 cttgatctct ttctcgtgct cggggaactc ctcggcgagg tcggccccga tcgcggcgga
     1441 gacgtcggcg atcgtcgcgc gtcgcttcgc cttctcggtg atcgagatcg cctcgcgcag
     1501 gcggtcgccc gcgagctgct cgacgcggga gaagagctcg tcggagtagt cgacgaacag
     1561 ggggtagtcc gggtcgtcgc cggtggtcgt ccccttcggc ttgccggcct gggcgacgag
     1621 ctcctgctgg agcgagatcg cctcggcgat gtactgcttg ctgaactcga tgccgtcggc
     1681 catgagctct tcgtcgggcg actgcgcgcc ggcctcgacg agctcgaacg cgtggtccga
     1741 cgccccggcc tcgatcatca tgatgtcgac ctcgccggcg tcgttgacgc gcccggcgac
     1801 gacgagctcg atcgtcgctt cctcctgctg catccacgtc gggttcgcga cccagctgcc
     1861 gttgatgtgc gagacgcgtg cggcgccgac cgggccctcg aacgggatgt ccgacagcgc
     1921 gagggcagcg gacgccgcgt tgatcgacag gatgtcgacc gggttctggc cgtcgaccgc
     1981 catcacgttg acgacgacgt ggacctcgtg gcggaagccg tcggggaagg cggggcgcag
     2041 cgggcggtcg atcagtcggg cgttgaggat cgccttctcg ctcgcgcggc cctcacggcg
     2101 gaagaacccg ccggggatct tgccggccgc gtacatgcgc tcctcgacgt cgaccgtgag
     2161 cgggaagaag tccgcgtcac cgcggggggc cgaggacgtg ccggctgcga agaccgtggt
     2221 ctcgccgacc gacgcgagca ccgcgccccc ggcctggccg gccaggcggc cggtctcgat
     2281 gcggaacacc tcgtcgccga tcttgcgctc gacgtacgtg ggctccgggg acataccagt
     2341 agtacctgta gacatgcgtc tcctcctgat gagttgggag gagaggcgcc agcctggccg
     2401 attctcagta gggggcgcac gagcaggacg tctcgtggat cctcaactga taatcgaccg
     2461 agggggccgg ccctctccga ttgcttgcag cggcttgggg ctccggcgtc ctcctttcgt
     2521 ccaggagtcc cgccgaggcg gccctcccgc aaatcgtggc ctttgggcct acctgcgcag
     2581 gcccagcttg gcgatgagcg cccggtagcg ctcgacgtcc tggcgttgca ggtacgcgag
     2641 cag
//
LOCUS       DASQDO010000401         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_103693, whole genome shotgun sequence.
ACCESSION   DASQDO010000401 DASQDO010000000
VERSION     DASQDO010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103693"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VEV43_11200"
     CDS             <1..1278
                     /locus_tag="VEV43_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24133.1"
                     /translation="AQIAIGTVEGREAVVLAGDWGCAVHDASTGDLILSDAVPTLALT
                     NAASGVMLLRHAPVGYAVVPLGRTSSTEYRLPDPSELPDLPLMALGEGESSLLLAVVS
                     RLGSGSVWNLRTGRRVGDVQFDIEVRAIAVSAVDRRTLVVAINDTDQEGGFVMSREAT
                     PVSLRRAAVARRPVRGVVGVGLVGEALVALERSGDTLELRSVPDGRPLPYDDEAVRVV
                     AGESVEHQTAALPRVSRMGLGEVRVERPEEWPPTASAVGDLGGRKVIVRGSYGGVVVV
                     FDATTGELVAAVQTELPQTVHLGTKGGPSAVSDVAVGSDLIAAAYEGSVLLFDVGLER
                     LPSDWIDAVAVRAVAVGAIDARMAVVATGGDGGGLRLWDVATRTRVASITLDVAVRRV
                     WLCGGVVVASTADGMLHAFDVRDRDARRAQPRD"
     gene            complement(1300..1372)
                     /locus_tag="VEV43_11205"
     tRNA            complement(1300..1372)
                     /locus_tag="VEV43_11205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1337..1339),aa:Ala,seq:tgc)
     gene            1469..>1719
                     /locus_tag="VEV43_11210"
     CDS             1469..>1719
                     /locus_tag="VEV43_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24134.1"
                     /translation="MEEGATETVERAESSEEDESPRAANRGEDQGGSESTPIRAEVSN
                     YEIVSHYFELAADRLEIPDDIRAVLRSAYREVQVQIPVRL"
BASE COUNT          240 a          579 c          654 g          246 t
ORIGIN      
        1 gcgcagatcg ccatcgggac ggtcgaggga cgtgaggcgg tcgtcctggc cggcgactgg
       61 ggttgcgccg tccacgacgc ctcgaccgga gacctgatcc tctccgacgc ggtccccacg
      121 ctcgctctga cgaacgccgc gagcggcgtc atgctgctca ggcacgcgcc agtcggatac
      181 gccgtcgtgc cgctcgggcg aaccagctcg accgaatacc ggttgcccga tccgtcggag
      241 ctgcccgatc tcccgctcat ggcgctcggc gagggcgagt cctcgctgct gctggcggtg
      301 gtgtcgcggt tgggcagcgg ctccgtctgg aacctcagga cggggaggcg cgtcggggac
      361 gtccaattcg acatcgaggt ccgcgcgatc gccgtcagcg ccgtggaccg gcgcacgctc
      421 gtcgtcgcca tcaacgacac ggaccaggaa ggcggcttcg tgatgagccg ggaggccacg
      481 ccggtgagcc tgcggcgcgc cgcggttgcg agacggcccg tccgcggcgt cgtcggagtg
      541 ggcctcgtcg gggaagcgct cgtcgcgctc gagcgctctg gagacaccct cgagctgcgc
      601 tcggtgccag acgggaggcc gctgccttac gacgacgagg cggtgagggt cgtggccggc
      661 gagtctgtgg agcatcagac ggccgcgctg ccccgtgtct cgcgcatggg tctcggcgag
      721 gtccgcgtcg agcgacccga ggagtggcct cccacggcga gcgccgtcgg ggacctggga
      781 ggccggaagg tcatcgtccg cggcagctac ggcggcgtcg tggtggtctt cgacgcgaca
      841 accggagagc tggttgccgc cgtgcagacg gagctaccgc agacggtgca cctcggcacc
      901 aagggtggcc cgtccgccgt gagcgacgtg gcggtcggct cggacctgat cgccgcggcg
      961 tacgagggct ccgtcctcct cttcgatgtc gggctcgagc ggctgccctc cgactggatc
     1021 gacgccgtcg cagtccgcgc cgtcgccgtc ggcgcgatcg acgcccggat ggccgtcgtg
     1081 gcgacgggtg gcgacggtgg aggccttcgc ctgtgggacg tcgcgacccg gacccgggtc
     1141 gcaagcatca cactcgatgt cgccgtgcgg cgggtctggc tgtgcggagg cgtggtcgtc
     1201 gcctcgacgg ctgacggcat gcttcacgcc ttcgacgtca gggatcgcga cgcccgccga
     1261 gcgcagccgc gagactgagc cgaccgaggg tcccgccaat ggagccagcc gggatcgaac
     1321 cggcgacctc ctgcttgcaa agcaggcgct ctcccagctg agctatggcc ccgcgtcggc
     1381 cgagtctagc caggctttgg catgagggca ggcggggcgg ggaacgtggg ggagcgggtc
     1441 gcatcgcatc ctgaggagga ggagacgcat ggaggaaggc gccacggaga cggtcgagcg
     1501 ggcggagtcg tcggaggaag acgagtctcc gcgcgcagcg aaccgcggcg aggaccaggg
     1561 cgggtcggag tccacgccca tccgcgcgga ggtctcgaac tacgagatcg tctcgcacta
     1621 cttcgagctg gccgccgacc ggctcgagat ccccgacgac atccgcgccg tcctgcgctc
     1681 ggcgtaccgc gaggtgcagg tccagatccc cgtgcggct
//
LOCUS       DASQDO010000402         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_470522, whole genome shotgun sequence.
ACCESSION   DASQDO010000402 DASQDO010000000
VERSION     DASQDO010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470522"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..904)
                     /locus_tag="VEV43_11215"
     CDS             complement(164..904)
                     /locus_tag="VEV43_11215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HYP24135.1"
                     /translation="MDARKIVGLVVACGLLVGASAAPVTAKKARSKVPAWGGEDSPIR
                     PGSSLGGYCTFNFVFYTPGTATKAPKAYIGTAGHCTDEIGEETELPGVGVIGKVVYDS
                     DLVEGEYTGVDFSLIEIDPKLVGKTNPTVLGWGGPTRSATVEDFAIGDQVDVHGYGLV
                     LGQNDVTRSRYGALVSWTEKEYQADMPAVNGDSGSPLIHHETGAALGIISRYGIGVPP
                     STDLGPLMTWIFEALDAEGFDVRLATVK"
     gene            complement(998..1453)
                     /locus_tag="VEV43_11220"
     CDS             complement(998..1453)
                     /locus_tag="VEV43_11220"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HYP24136.1"
                     /translation="MTLYEFLKFVHVLLAIVAVGFNASYGIWIARAAKEPEHELHILR
                     GIKFLDDRIANPAYGLLLVIGIALVFVGNWEITTFWILTSLILYAVVTVLAITTYSPA
                     LRQQIALLEAGRGKSDEYAALSKRAGMVGGLMGALVVAIVFLMVTKPTL"
     gene            1457..1906
                     /locus_tag="VEV43_11225"
     CDS             1457..1906
                     /locus_tag="VEV43_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24137.1"
                     /translation="MSLASGARSLPDRRHITALFRVLLAGVVLTTIHYTDNYLYFEEY
                     PQPGSLQRWHVYAAWLLLTAAGVVGYALYERGLVAPAYVCLVVYSYTGLSSLGHYLYG
                     PWAEFSVKQHAFILVDGLTGSAVIAFVVWSALTRRRRDSTRALGGIA"
     gene            1979..2185
                     /locus_tag="VEV43_11230"
     CDS             1979..2185
                     /locus_tag="VEV43_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24138.1"
                     /translation="MPDEKEIKYRDDSGQEVTEKVDAETLDEKMNEADAVYGVPGEDK
                     RRAAYLAQSPDDKPDEEPTGERNS"
     gene            2208..>3064
                     /locus_tag="VEV43_11235"
     CDS             2208..>3064
                     /locus_tag="VEV43_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYP24139.1"
                     /translation="MRRVPAALRRLVLPEPRGPLSAHVIDALGGPVRRLEHHPDVSGD
                     ALAGDDFHLALYLCYEVHYADAGDPGWEWEPTLLEFRRTLEDAFEKDLVTSLSPGTLD
                     GDLPARLIALARSEDGRSLSRYLAGDPTFDRVTELVIHRSAYHLKEADPHSWAIPRLT
                     GAAKAALVEIQADEYGGGDPASMHSALFAQTMRALGLDDTYGAYLSRLPGITFANVNL
                     MSMFGLHRRWRGAAAGHLALFEMTSTEPNRRYGDALRSLWFGTKATAFYDEHVEADAV
                     HENVAAFDLA"
BASE COUNT          481 a         1058 c         1067 g          458 t
ORIGIN      
        1 ctcgacggag gaacggccga gcgggacgat gcggttccct gtgccgagcc cccggtggcg
       61 cccgccctcg agggacgcga ggccgtagtc gcgttcgaag cgggccgccg cggcggcgag
      121 gtcgccggcc gcgacgatga cgtggtctag ctcgaccaaa cggctacttg acggtggcga
      181 gccggacgtc gaagccctcg gcgtcgagcg cctcgaagat ccacgtcatg agggggccga
      241 ggtccgtcga cggggggacg ccgatgccgt agcggctgat gatcccgagc gccgcaccgg
      301 tctcgtggtg gatcagcgga gagccactgt ctccgttcac cgccggcatg tccgcctggt
      361 actccttctc ggtccacgag acgagcgcgc cgtagcggga gcgggtgacg tcgttctgcc
      421 ccagcacgag gccgtacccg tggacgtcga cctggtcgcc gatagcgaag tcctcgaccg
      481 ttgcggagcg ggtcgggcca ccccagccga gcaccgtcgg gttggtcttg ccgacgagct
      541 tggggtcgat ctcgatgagc gagaagtcga cgccggtgta ctcgccctcg acgaggtccg
      601 agtcgtagac gaccttgccg atgacgccga cgccggggag ctcggtctcc tcgccgatct
      661 cgtcggtgca gtggccggcc gtgccgatgt acgccttcgg cgccttggtc gcggtgccgg
      721 gcgtgtagaa gacgaagttg aacgtgcagt acccaccgag cgacgagccc gggcggatcg
      781 gagagtcctc gccgccccac gccggaacct tgctgcgggc cttcttcgcg gtgacggggg
      841 ctgccgaggc tcctacgagc aggccacatg ccactacgag gccgacgatc ttcctggcgt
      901 ccaacgcgtt ctccttgggt cggggggtcg cggggaaagg ttctccccgt gcgggtcttc
      961 ccctgccggg gggccgcgga cggccccgcc gcgggcgcta gagcgtcggc ttcgtgacca
     1021 tcaggaagac gatggccacg acgagggccc ccatgaggcc gccgaccatc ccggcccgct
     1081 tcgacagcgc cgcgtactcg tcggacttcc cgcgccccgc ttcgagcaga gcgatctgct
     1141 gccgcagcgc cggcgagtac gtcgtgatcg cgaggacggt gacgaccgcg tacaggatca
     1201 gcgacgtgag tatccagaaa gtggtgatct cccagttgcc cacgaacacg agcgcgatcc
     1261 cgatcacgag cagcaggccg taggccgggt tcgcgatgcg gtcgtcgagg aacttgatcc
     1321 cgcgcaggat gtggagctcg tgctccggct ccttggcggc gcgggcgatc cagatcccgt
     1381 aggacgcgtt gaagccgacg gcgacgatcg cgagcagcac gtggacgaac ttgaggaatt
     1441 cgtagagagt catgacgtga gcttagcgag cggagcgagg tcgttgccgg atcgccgcca
     1501 catcacggcg ctgttccgcg tgctgctcgc cggggtcgtg ctgacgacga tccactacac
     1561 cgacaactac ctctacttcg aggagtaccc gcagccgggg tcgctgcagc gctggcacgt
     1621 ctacgccgcg tggctcctgc tcacggccgc cggcgtcgtc ggatacgcgc tctacgagag
     1681 aggtctcgtc gcgcccgcgt acgtctgcct ggtcgtctac tcgtacaccg ggctgagcag
     1741 cctcggtcac tacctctacg ggccgtgggc cgagttcagc gtcaagcagc acgcgttcat
     1801 cctcgtcgac ggcctcacgg gctcggcggt gatcgcgttc gtcgtctggt cggcgctgac
     1861 ccgccgccgc cgggattcaa cccgggccct cgggggaatc gcgtagacga ccggccggcg
     1921 gtttgccccg tccgggcgtg gagagaaagc aagcaaagga ctagccgagg aggcagggat
     1981 gcccgacgag aaggagatca agtaccgcga cgactccggc caggaggtca cggagaaggt
     2041 cgacgccgag acgctcgacg agaagatgaa cgaggccgac gccgtctacg gcgtcccggg
     2101 cgaggacaag cgccgcgccg cgtacctcgc gcagagcccc gacgacaagc ccgacgaaga
     2161 gccgaccggt gagcgcaact cctgacgcgc cgccacacgt tccttccgtg agacgcgtac
     2221 cggcggccct ccgccgcctc gtcctgcccg agccgcgcgg ccccctcagc gcacacgtga
     2281 tcgacgcgct cgggggtccg gtgaggcggc tcgagcacca tccggacgtg agcggtgacg
     2341 cgctcgcggg cgacgacttc cacctcgcgc tgtacctctg ctacgaggtc cactacgccg
     2401 acgccggtga cccggggtgg gagtgggagc cgaccctgct cgagttccgc cgcacgctgg
     2461 aagacgcgtt cgagaaggac ctcgtcacgt cgctgtctcc ggggaccctc gacggagacc
     2521 tccctgcgcg cctgatcgcc ctggcgcgct ccgaagacgg acggtcgctc agccggtacc
     2581 tcgccggcga cccgacgttc gaccgcgtca ccgagctcgt gatccaccgg tccgcgtacc
     2641 acctgaagga ggcggacccc cattcgtggg ctatcccgcg cctcacgggt gcggcgaagg
     2701 ccgcgctcgt cgagatacaa gccgacgagt acggaggcgg cgatcccgcg tcgatgcact
     2761 cggccctgtt cgcgcagacg atgcgcgcgc tcggcctcga cgacacctac ggcgcgtacc
     2821 tgtcgcgtct gccgggaatc acgttcgcga acgtgaacct gatgtcgatg ttcggcctgc
     2881 accggcgctg gcgcggcgcg gcggccggcc acctcgcgct gttcgagatg acgtccaccg
     2941 agcccaaccg ccgctacggc gacgcgctgc ggagcctgtg gttcggtacc aaggccacgg
     3001 cgttctatga cgagcacgtc gaggccgacg cggtccacga gaacgtcgcc gcgttcgacc
     3061 tcgc
//
LOCUS       DASQDO010000403         6081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_103761, whole genome shotgun sequence.
ACCESSION   DASQDO010000403 DASQDO010000000
VERSION     DASQDO010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103761"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VEV43_11240"
     CDS             complement(<1..729)
                     /locus_tag="VEV43_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HYP24140.1"
                     /translation="MFDSLTGRFDDIFTRLRSRGRLSEKQVDEVLREIRLALLEADVS
                     LKVVKAFVADVRQRAEGAEIHKSLTPAQQVIKLVHGALIDVIGHEAVGLEPSPRPPRV
                     ILMAGLQGSGKTTASGKLARHLKKSGRRPVLAACDLRRPAAISQLRILGEQVGVPVFS
                     EEGTVDAPAVAARALAHARAEGYTDLIVDTAGRMHVDPDLMAELGSVSKEVSPTETLL
                     VCDAMTGQDAVNVAEAVLEEVEVTG"
     gene            complement(734..1066)
                     /locus_tag="VEV43_11245"
     CDS             complement(734..1066)
                     /locus_tag="VEV43_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24141.1"
                     /translation="MRLPWQVPDDPERRAMMPRLDLRERLRLALKVPVPFVAYIWFQT
                     DQTAASALVAFAGAVGTLSAIELVWLRTTTPAERIFTKTKRQAVFLHVAVLAACAAAL
                     VVAIPRLR"
     gene            complement(1063..1344)
                     /locus_tag="VEV43_11250"
     CDS             complement(1063..1344)
                     /locus_tag="VEV43_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24142.1"
                     /translation="MVRPEPNRPPRIDERYRMFLRIVVVLVAYGAYLLVQGERLMAGV
                     LVGAGVIALGFSTVDRLTRWSHDRSDMMLVATTLLGLGLTAIGLFLYLR"
     gene            complement(1354..2250)
                     /locus_tag="VEV43_11255"
     CDS             complement(1354..2250)
                     /locus_tag="VEV43_11255"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYP24143.1"
                     /translation="MNVVVAGGTGFIGRHVVRALIEAGHRVSVMSRNPASVGRIRELE
                     GASALQGDLTDPRTLTGKLNGAEAVVASVQFPNHPVEVPRKGLTYDRYDRRGTENLVA
                     EAVDAGVRRFFYLSGAGADPKSPVVWYRAKGRAEESLRASGLEWAALRPSWAYGPEDR
                     ALNKFAFMARYLPVVLQVGVKPQRVQPVWIGDLALAVRRTFERDAWNAVYEIGGPDVM
                     TMNEIIHTMLDVMGKRRAVIPVPTWLAKAGTMPLRLLPSPPMTPQGIEFATQPGLVDN
                     TLVRERLGVQTIGLREGLSHYL"
     gene            complement(2247..3416)
                     /gene="ftsY"
                     /locus_tag="VEV43_11260"
     CDS             complement(2247..3416)
                     /gene="ftsY"
                     /locus_tag="VEV43_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYP24144.1"
                     /translation="MPEGIVPELLIALALLGTIGGALFVSRRGRDDEPPGEPRGGVDL
                     KERPDDAVQTIAPPVEAPPVEEAPPVAAPPVEIVPEEPLPVEAPRPTLRDRLSKSRKF
                     FSDRLADAFGRAPDDETWEDVEAALIQADVGVDTATRITEALKDRARERKVTQPEEVK
                     ALLAEELVALFDADRDRGLTMADSGAGGGPTVWLVVGVNGTGKTTTIGKLSRDLSERG
                     RSVSLAAADTFRAAADEQLGVWAERTGAQLVKHQHGADPGAVAFDAFQHARARDVDVL
                     IVDTAGRLHTKTPLMDELGKVRRVIEKEGEVHETLLVIDATAGQNGLVQAREFAQAAG
                     VTGVVLTKLDGTAKGGIALAIEQDLGIPIKLVGVGEGVDDMEPFDPKTFVDALLA"
     gene            3531..4271
                     /locus_tag="VEV43_11265"
     CDS             3531..4271
                     /locus_tag="VEV43_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYP24145.1"
                     /translation="MVVVVLFGLVLAVEVWLALRREYLPTDEPLDLDRVFGASDERPL
                     RLVVLGDSTAAGIGVDDPADAYPALLARLLATTTGRPVKLTALGVAGARVEDVATSQA
                     PAAAAAEPDLVFVGIGANDVTHLTSLGDVRDDMERALRILDEAGAAVVVAGPPDMRVP
                     VWHQPLRQLSYLRGIQVAGAIEDVAAERGVGFVELAEETGPFFEADPDAHFSTDGFHP
                     GPLGYRRWADAIFPVLIEAAKEAGLVEN"
     gene            complement(4268..>6081)
                     /locus_tag="VEV43_11270"
     CDS             complement(4268..>6081)
                     /locus_tag="VEV43_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP24146.1"
                     /translation="RREVAERELGADATKAAAAEEEVRNLETELDRVTEAAAAAEMSL
                     EEARERADLLKASLGATHAERAAAAARLDTLREVDALFADSPVRERLDPLIEAAEASA
                     QMATAGEEEAAAILAEAETEAERRWGEVAKQDEELRRLDALLSAAADRLAGRRRRHEA
                     REVELAALDEELSRARDALATAERAATEERAALPERKAAVEVARDRREAAERRLATAR
                     EAASVSAAAATEQEVAARGAEERAAAARQRLAEAESGIAAAEAALEGLEDLRATLDAK
                     RRRAEQVAFAATDAAARAAAWARDADVRADRARERQRAGETQLESLRARERELGKSLE
                     EVARRRNAAEVRRAETRARAAAVAERSLEEWGLTADDLRALGRLEPDAEAEARTLVDE
                     LDRQMKRLGTVNPRAAEEYAELAERESFLESQMTDLRTSKRDLMAIVTEVDETIVEVF
                     GQAFADVAREFEGVIQRLFPGGSGELRLTNPDDVLTSGIEIEARPPGKNVRKLSLLSG
                     GERSLVALAFLFSIFRSRPSPFYLLDEVEAALDDVNLQRFLGLVSELEERAQILIVTH
                     QKRTMEAADILYGVSMAKDGVSHVVAQRMEDVAVAQP"
BASE COUNT          814 a         2249 c         2098 g          920 t
ORIGIN      
        1 gcccgtcacc tcgacctctt cgaggaccgc ctccgcgacg ttgaccgcgt cctgacccgt
       61 catcgcgtcg cacacgagca gcgtctcggt cggcgagacc tccttgctga cgctgccgag
      121 ctcggccatc aggtcggggt cgacgtgcat gcgtcccgcg gtgtcgacga tgaggtcggt
      181 gtagccctcg gcgcgtgcgt gagcgagagc ccgcgccgcg accgccggag cgtcgaccgt
      241 cccctcctcg ctgaacacgg ggacgcccac ctgctcgccg aggatcctca gctggctgat
      301 cgcggcaggc ctgcggaggt cgcacgccgc gaggaccggc cgccgcccgc tcttcttcag
      361 gtgacgggcg agctttcccg acgcggtcgt cttgccggag ccctggaggc ccgccatcag
      421 gatcacgcgc gggggccggg gcgacggctc gagccccacc gcctcgtgac cgatcacgtc
      481 gatgagggca ccgtgcacga gcttgatgac ctgctgcgcc ggcgtgaggc tcttgtggat
      541 ctcggccccc tcggcgcgct ggcgcacgtc ggcgacgaaa gccttcacga ccttcagcga
      601 gacgtcggcc tcgagcagcg cgaggcggat ctcgcgcagg acctcgtcga cctgcttctc
      661 ggacaggcgg ccgcgcgacc gcaggcgcgt gaagatgtcg tcgaagcgcc cggtcagtga
      721 gtcgaacatc gcctcagcgt aagcgaggta tcgcgactac cagggcggca gcgcacgccg
      781 cgaggaccgc gacgtgcagg aagacggcct gccgcttggt cttggtgaag atgcgctccg
      841 caggcgtcgt ggtgcgcaac cacacgagct cgatcgccga caacgtcccc actgcgcccg
      901 cgaacgcgac gagagccgaa gccgcggtct ggtctgtttg gaaccagatg tacgcgacga
      961 agggaacggg caccttgagc gcgagccgca gcctctcgcg caggtcgagc cgcggcatca
     1021 tcgcccgtcg ttcgggatcg tcggggacct gccacggaag cctcaccgca ggtacaggaa
     1081 cagccctatg gcggtgaggc cgagcccgag cagagtggtc gcgacgagca tcatgtcgga
     1141 gcggtcgtgg ctccagcgcg tcaagcggtc gaccgtcgag aacccgaggg cgatcacccc
     1201 cgcgccgacg aggacgcccg ccatcagcct ctcgccctgg accaacaggt acgcgccgta
     1261 cgcgacgagg accacgacga tccgaaggaa catccggtag cgctcgtcga tgcggggcgg
     1321 gcggttcggc tcgggacgga ccatcgcgct ctcctagagg tagtgcgaga ggccctcgcg
     1381 cagaccgatg gtctgcacgc cgagccgctc gcggacgagg gtgttgtcga cgaggccggg
     1441 ctgcgtcgcg aactcgatcc cctgcggggt catcggcggg ctcggcagca gccgcagcgg
     1501 catcgtgccg gccttcgcca gccaggtcgg gaccgggatc accgcacgcc gtttgcccat
     1561 cacgtcgagc atcgtgtgga tgatctcgtt catcgtcatc acgtccgggc cgccgatctc
     1621 gtagaccgcg ttccatgcgt cgcgctcgaa cgtccgtcgc acggcgagcg cgaggtcgcc
     1681 gatccatacg ggctggacgc gctggggctt cacgccgacc tggagcacga cggggagata
     1741 gcgcgccatg aaagcgaact tgttcagagc gcggtcctcg gggccgtagg cccacgaggg
     1801 cctgagcgcg gcccactcga gcccgctcgc tcgcagcgac tcctcggcgc ggcccttcgc
     1861 gcggtaccag accaccgggc tcttcgggtc ggcgcccgca ccgctcaggt agaagaaccg
     1921 gcgcacgccc gcgtcgacgg cctccgcgac gaggttctcg gtcccccgcc ggtcgtagcg
     1981 gtcgtaggtg agccccttgc gcgggacctc gacgggatgg ttcgggaact ggacggacgc
     2041 gacgacggct tcggccccgt tcagctttcc ggtcagcgtc ctgggatcgg tgaggtcacc
     2101 ctggagcgcg gacgcgcctt cgagctcccg gatccggcct accgaagcgg ggttgcgcga
     2161 catcaccgag acgcggtgcc ccgcttcgat cagtgcccgg acgacgtgcc ggccgatgaa
     2221 gcccgtcccg ccggcgacga cgacgttcac gccaggagcg cgtccacgaa ggtcttgggg
     2281 tcgaacggct ccatgtcgtc gacgccctct ccgacgccga cgagcttgat cgggatgccg
     2341 aggtcctgct cgatcgcgag cgcgatgccc cccttcgcgg tgccgtcgag cttcgtcagc
     2401 acgacaccgg tcaccccggc ggcctgcgcg aactcgcgcg cctggacgag cccgttctgg
     2461 cccgcggtcg cgtcgatcac gaggagggtc tcgtgcacct cgccctcttt ctcgatcacg
     2521 cggcggacct tgcccagctc atccatcaac ggggtcttgg tgtgcagccg tcccgcggtg
     2581 tcgacgatca ggacgtccac gtcgcgggcg cgcgcatgct ggaacgcgtc gaacgcgacc
     2641 gctccggggt ccgcgccgtg ctggtgcttg acgagctggg cccccgtgcg ctcggcccac
     2701 acgcccagct gctcgtcggc ggcggcgcgg aacgtgtcgg ccgcggccag cgagaccgag
     2761 cgtcctcgct ccgagaggtc gcgcgagagc ttcccgatcg tcgtcgtctt gcccgtgccg
     2821 ttcacgccga cgacgagcca cacggttggt cccccgcccg cccccgaatc ggccatcgtg
     2881 agcccgcggt cacggtcggc gtcgaacaac gcgacgagct cctcggcgag aagcgccttc
     2941 acttcctcgg gctgcgtcac cttgcgctcg cgagcgcggt ccttcagcgc ttcggttatc
     3001 cgcgtcgcgg tgtctacgcc gacgtcggcc tggatcagcg cggcctcgac gtcctcccac
     3061 gtctcgtcgt cgggcgctcg gccgaacgcg tcggcgagac ggtcggagaa gaacttgcgg
     3121 ctcttggaga ggcggtcgcg cagtgtcggc cgaggagcct cgacgggcag cggttcctcg
     3181 gggacgatct cgaccggagg ggccgcgacg ggtggcgcct cctcgacggg aggggcctcg
     3241 accgggggcg cgatcgtctg aacggcgtcg tcggggcgct ccttcaggtc gacgccgccg
     3301 cggggctcgc ccggcggctc gtcgtcgcgg ccgcgccgag acacgaacaa cgcgccgccg
     3361 atggtgccga gcagcgcgag cgcgatcagt agctcaggaa cgatcccctc aggcatggcg
     3421 gcaaggctaa acgacgtccc tcaccggttg agcccacgta cgctggcgac atcgcagagc
     3481 gtccgggggc ccgcagcagg agactcgtct tccgcgccct gctttcggtc gtggtcgtcg
     3541 tcgtcttgtt cgggctcgtt cttgcggtcg aggtctggct cgcgttgcgg cgcgaatacc
     3601 tgcctaccga cgagcccctc gacctcgacc gcgtcttcgg cgctagcgac gaacggccgc
     3661 tgcgcctcgt cgtcctcggc gattccaccg ctgccgggat cggcgtcgac gaccccgcgg
     3721 acgcgtaccc ggccttgctc gcacgcttgc tggccacgac gacgggccgg ccggtgaagc
     3781 tcacggccct gggtgttgcc ggcgctcggg tcgaagacgt cgcgacgtcg caggccccgg
     3841 cggccgccgc ggccgagccc gacctcgtct tcgtcgggat cggcgccaac gacgtcacgc
     3901 acctgacgtc actcggcgac gtacgcgacg acatggaacg cgcgctccgg atcctcgacg
     3961 aagcgggggc cgcggtcgtc gtcgcgggcc ctcccgacat gcgcgtgccg gtgtggcatc
     4021 agccgctacg gcagctttcg tatctccgcg gcatccaggt ggccggcgcc atcgaagacg
     4081 tcgccgccga acggggtgtc ggattcgtcg agctggccga ggagaccggc ccgttcttcg
     4141 aagccgaccc cgacgcccac ttcagcaccg acggcttcca tcccggcccc ctcggctacc
     4201 gccggtgggc ggacgcgatc ttcccggtgc tgatcgaggc tgcgaaggaa gcagggctcg
     4261 tagagaacta gggctgcgcg accgcgacgt cttccatgcg ctgcgcgacc acgtgcgaga
     4321 cgccgtcctt cgccatcgag acgccgtaca ggatgtcggc ggcctccatc gtgcgcttct
     4381 ggtgggtgac gatcaggatc tgcgcgcgct cctcgagctc gctcacgagg cccagaaacc
     4441 tctgcaggtt cacgtcgtcg agtgcggcct cgacctcgtc gagcagatag aagggcgacg
     4501 gcctcgaccg gaagatcgag aacaggaacg cgagcgccac caacgaccgc tcgccacccg
     4561 agagcagcga cagcttgcgg acgttcttgc ccgggggccg ggcctcgatc tcgatcccgc
     4621 tcgtcaggac gtcgtccggg ttcgtcagcc tgagctcgcc ggacccgccc gggaacagcc
     4681 tctggatgac gccttcgaac tcccgcgcga cgtctgcgaa cgcctggccg aacacctcga
     4741 cgatcgtctc gtccacttcc gtcacgatcg ccatcaggtc gcgcttgctc gtgcgcaggt
     4801 cggtcatctg cgactcgagg aacgactcgc gctccgcaag ctcggcgtac tcctcggcgg
     4861 cgcgcgggtt gacggtgccg agcctcttca tctggcggtc gagctcgtcg accagcgtcc
     4921 tggcctcggc ctctgcgtcc ggctcgagcc gtcccagcgc gcgcaggtcg tcggccgtta
     4981 gtccccactc ctcgagagag cgctcggcga cggcagcagc gcgggctcgc gtctcggccc
     5041 ggcgcacctc ggccgcgttg cggcggcgcg ccacctcttc gaggctcttg ccgagctcgc
     5101 gctcgcgcgc ccgcaacgac tcgagctgcg tctcccccgc ccgctggcgt tcccgcgccc
     5161 ggtcggcccg cacgtccgcg tcgcgcgccc acgcagccgc gcgtgccgcc gcgtcggtcg
     5221 cggcgaacgc gacctgctcg gcccgccggc gcttcgcgtc gagcgtcgcc cgcagatcct
     5281 cgagcccctc gagcgcggcc tccgcggccg cgatcccgga ctcggcctcg gcgaggcgct
     5341 ggcgggcggc ggcggctcgc tcctcggcgc cccgagccgc aacctcctgc tccgtcgcgg
     5401 ccgcagccga caccgacgcc gcttcgcgag cggtcgcaag tcgtcgttcg gccgcctcgc
     5461 ggcggtcgcg ggcaacctcg accgcggcct tgcgctcggg cagggctgcc ctctcctccg
     5521 tcgcggcacg ttcggcggta gcgagggcgt cgcgcgcccg ggacagctcc tcgtcgagag
     5581 cggcgagctc gacttcgcgt gcctcgtgcc ggcgacgccg ccccgcaagc ctgtccgcgg
     5641 cggcggagag cagagcgtcg agccttcgca gctcctcgtc ctgcttcgcg acctcgcccc
     5701 agcggcgctc ggcttcggtc tcggcctcgg cgaggatcgc cgccgcctcc tcttcgcctg
     5761 ccgtcgccat ctgcgcggag gcctcggccg cctcgatcag cgggtcgaga cgttcccgga
     5821 cgggcgagtc ggcgaacagg gcgtcgacct cacgaagcgt gtccagtcga gcggcggccg
     5881 cggcccgttc ggcgtgggtc gcaccgagcg acgccttgag caggtcggcg cgctcgcgcg
     5941 cctcctccaa cgacatctcg gcagcggccg cagcttcggt gacgcggtcg agctcggtct
     6001 cgaggttccg gacctcctcc tcggcggcag ccgccttggt ggcgtcggct ccgagctcgc
     6061 gctcggcgac ctctcgcctc t
//
LOCUS       DASQDO010000404         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_960315, whole genome shotgun sequence.
ACCESSION   DASQDO010000404 DASQDO010000000
VERSION     DASQDO010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960315"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..346)
                     /locus_tag="VEV43_11275"
     CDS             complement(44..346)
                     /locus_tag="VEV43_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HYP24147.1"
                     /translation="MFIGVARLELFIPDATSLKDKRHVLRSVTDGVRRKFNVSVAEVA
                     YQDLWQRTAIAVACTSETAGQCRKVLQEVEKAVGRAAIDGAEIVDRSIEVVSWEDL"
     gene            complement(360..>2413)
                     /gene="infB"
                     /locus_tag="VEV43_11280"
     CDS             complement(360..>2413)
                     /gene="infB"
                     /locus_tag="VEV43_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYP24148.1"
                     /translation="EVAKELGLSSKEVLAHLEQIGQPAKSHSSSIEEELAARVRTDLG
                     NGAAPAAEAPAPAEPPPVEAPPPPVAEPVADPEPEPEPEPEPEPEPEPAAVADDVIRV
                     HRGITVKDFAEALDRSPNEVVKVLIGMGEMVTVTQSLSDEAVLLVADELGASVSVIDP
                     AQEEAEADAAEGDDEEDGELITRPPVVTVMGHVDHGKTAILDAIRKTDVAGGEAGGIT
                     QHIGAYRVHYDGREVTFIDTPGHEAFTAMRARGAQVTDIAILVVAADDGVMPQTVEAI
                     DHAKAARVPIVVAVNKIDKPEADPMRVRTQLSDHGLQPEEWGGDTVFVDVSAKQRTNL
                     DGLIEMIMLTADVQLEGHLKANPTTHARGVVIEAHLDKGRGPLATVLVKKGTLYQGDA
                     IIAGASWGRVRAMLDERGNNVEEAAPGQPVQVLGWQSVPEAGDEFRSLDDEREARAIA
                     GEREQHRRAAELVSARGQSLQSLLAATREGEIPELNLLLKADAQGSVEALDDQLAKLD
                     QSVVKVNVLRKGVGGITENDITLAQASDAIVIGFNVVPNVQARTLAEEGGVDVRTYRV
                     IYQIVQDVENAARGLLGPEEREVALGQAEVRATFRVPRLGVVAGCMVLDGVIRRNAAA
                     RLVRDGTVVYETTIGSLRRFKDDQREVAAGFECGIGLEGFQDVKEGDIIQAFEVQEVA
                     R"
BASE COUNT          295 a          873 c          834 g          411 t
ORIGIN      
        1 agcacctggc gtgcgagctt ctgcaccctc tccgtccgct ggctcacagg tcctcccacg
       61 acacgacctc gatcgaacgg tcgacgatct cggcgccgtc gatcgcagcc ctcccgacgg
      121 ccttctcgac ctcctgcagc accttgcggc actgaccggc ggtctcggac gtgcacgcca
      181 cggctatcgc cgtgcgctgc cacaggtcct ggtacgcgac ctccgcgacg gagacgttga
      241 acttcctgcg gacgccgtcc gtgaccgacc gcagcacgtg tcgcttgtcc ttcagcgacg
      301 tggcgtccgg gatgaagagc tctagccgag cgacacctat gaacatctgg tcgaacgggc
      361 tagcgcgcta cctcttggac ctcgaacgcc tggatgatgt cgccctcctt gacgtcctgg
      421 aagccctcga ggccgatgcc gcactcgaag cccgcggcca cctcgcgctg gtcgtccttg
      481 aagcgcctga gcgagccgat cgtcgtctcg tacacgacgg tcccgtcgcg gacgagccgc
      541 gctgccgcgt tcctccggat gacgccgtcg agcaccatgc aaccggcgac cacgccgagc
      601 ctcggcaccc ggaacgtcgc gcgcacctcg gcctggccga gcgcgacctc gcgctcctcg
      661 gggccgagca gaccgcgagc cgcgttctcg acgtcctgca cgatctgata gatcacgcgg
      721 tacgtgcgga cgtcgacgcc gccctcctcg gccagcgtcc gcgcctgcac gttcggcacg
      781 acgttgaagc cgatgacgat cgcgtcggag gcctgcgcga gcgtgatgtc gttctcggtg
      841 atcccgccga cgcccttgcg cagcacgttg accttgacca ccgactggtc gagcttcgcg
      901 agctggtcgt cgagcgcctc gaccgagccc tgtgcgtcgg ccttcagcag caggttgagc
      961 tcggggatct cgccctcgcg cgtcgcggcg agcagggact ggagcgactg gccgcgggcg
     1021 gagacgagct cggccgcgcg ccggtgctgc tcgcgctcgc cggcgatcgc ccgggcctcg
     1081 cgctcgtcgt cgagcgaacg gaactcgtcg ccggcctcgg gcaccgactg ccacccgagc
     1141 acctggacgg gctggcccgg cgccgcctct tcgacgttgt tgccgcgctc gtcgagcatc
     1201 gcgcggacac ggccccacga cgccccggcg atgatcgcgt cgccctggta gagcgtcccc
     1261 ttcttgacga ggacggtcgc tagcgggccg cggcccttgt cgaggtgcgc ctcgatgacg
     1321 acgcctcgtg cgtgcgtggt cgggttcgcc ttcaggtggc cttcgagctg gacgtccgcg
     1381 gtgagcatga tcatctcgat cagcccgtcg aggttcgtcc gctgcttcgc gctgacgtcg
     1441 acgaacacgg tgtcgccgcc ccactcctcg ggttggaggc cgtggtcgga gagctgcgtg
     1501 cggacgcgca tcgggtcggc ctcgggcttg tcgatcttgt tcacggcgac gacgatcggg
     1561 accctcgccg ccttggcgtg gtcgatcgcc tcgaccgtct gcggcatgac gccgtcgtcg
     1621 gcggcgacga ccaggatcgc gatgtcggtc acctgggcgc cgcgggcgcg catcgcggtg
     1681 aacgcctcgt ggccgggggt gtcgatgaac gtgacctcgc ggccgtcgta gtggacgcgg
     1741 tacgcaccga tgtgctgcgt gatgccgccg gcctctccgc cggcgacgtc ggtcttgcgg
     1801 atcgcgtcga ggatcgcggt cttgccgtgg tcgacgtggc ccatgaccgt gacgaccggg
     1861 ggccgcgtga tgagctcgcc gtcctcctcg tcgtcgccct cggcggcgtc ggcctcggcc
     1921 tcctcctggg cggggtcgat gaccgagacg ctggcgccga gctcgtcggc gacgagcagc
     1981 acggcctcgt cgctcagcga ctgggtgacc gtgaccatct cgcccatccc gatgaggacc
     2041 ttcacgacct cgttgggtga gcggtcgagg gcctcggcga agtccttgac cgtgatgccc
     2101 cggtgcacgc ggatgacgtc gtccgccaca gcggcgggct cgggctccgg ctcgggctca
     2161 ggttccggct cgggttcggg ctccggatct gctacgggct ccgcgaccgg aggcggaggc
     2221 gcctcgacgg gggggggctc ggcaggcgcg ggggcctcgg ccgcgggcgc ggccccgttg
     2281 cccaggtcgg tccggacgcg cgcggccagc tcttcctcga tgctcgagct gtggctcttc
     2341 gcgggctgcc cgatctgctc gaggtgcgcg agcacctcct tcgagctgag ccccagctct
     2401 ttcgctactt cgt
//
LOCUS       DASQDO010000405         6824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_103920, whole genome shotgun sequence.
ACCESSION   DASQDO010000405 DASQDO010000000
VERSION     DASQDO010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103920"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VEV43_11285"
     CDS             <1..322
                     /locus_tag="VEV43_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24149.1"
                     /translation="VAALLVNAAAGLGYRVYRFRKGGPKSDVIGQAILGAVLVGLAAG
                     LAAGAGWPRWPALVYGLLFGIIVMPIWVLAVLIPLGPRAIDYAFTVVYWVLLAVVVVA
                     GLAL"
     gene            complement(328..816)
                     /locus_tag="VEV43_11290"
     CDS             complement(328..816)
                     /locus_tag="VEV43_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HYP24150.1"
                     /translation="MSNGDYAKSLGESLRRIRVQKGMSLQDVQQASDGKWKAAVVGAY
                     ERGDRNVTVARLSELAEFYGVPVSELLPDDGAPPAPGQVETRRRVVLDLEGLTKVPEA
                     DRDPLSRFTTAIQIQRGDFNGRVLTIREDDLMALALLYQTTSDELSRRLADWGLLSKN
                     GA"
     gene            955..1389
                     /locus_tag="VEV43_11295"
     CDS             955..1389
                     /locus_tag="VEV43_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYP24151.1"
                     /translation="MKTLWKGAITFGLISIPVRLYSAVQEKSLKFHLLHGEDKGRIKY
                     KRECAKCGKEVGWDDIIKGYEFAKDQYVTFSEEELDALDVDSIKAIDVVAFVPLEDID
                     PVYFNKTYYVGPEATGVKAYRLLADALEAEGQVGVAKVALRD"
     gene            1441..1782
                     /locus_tag="VEV43_11300"
     CDS             1441..1782
                     /locus_tag="VEV43_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24152.1"
                     /translation="MHWPDEIRQPDFEELDKKVEVRDTEVKMARQLIQQLSSSWDPEA
                     FRDEYRAALEELVEKKVEGQEIAIAAEPEEEPTKVVDLMEALKASVEQAKKAKESGAG
                     KKAAKPKASAG"
     gene            1816..2181
                     /locus_tag="VEV43_11305"
     CDS             1816..2181
                     /locus_tag="VEV43_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HYP24153.1"
                     /translation="MVVPATPSYLQVLRLVAAGLASRLAFSIDEIEDLKIAVDEMSAY
                     LTGSQGRDGTIEIAFEVDTSSITIRGTGRFEPGHKVRAGLTDFSKMILETVVDSASLE
                     QSDGIPTFTLVKSKQLETT"
     gene            2256..2948
                     /locus_tag="VEV43_11310"
                     /pseudo
     CDS             2256..2948
                     /locus_tag="VEV43_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
     gene            2948..5068
                     /locus_tag="VEV43_11315"
     CDS             2948..5068
                     /locus_tag="VEV43_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HYP24154.1"
                     /translation="MRALRIAALGTVVALVAGGCSLRPVDLDEQKPLALRSTFRAADG
                     SVLARFFKENRKLVELDDLPRSLIDAVVAAEDARFFEHPGFDVRAIARAALVNLAEGE
                     TVQGGSTITQQYVKNTFFRKPAKTLERKAKELRLAIEVEKRYDKREILELYLNTVYFG
                     RGAYGVATASESYFGHGVRDLTLSESALLAAVIRAPSRYDPREHPRRALGRRDYVLDR
                     METLGKVTPRRAAAARRRPLGVVPVSAPPEARYPYFVEAGRQELLADSRLGRSDDERA
                     RAIYEGGLVVKTTLDPQMQAAAERAVADVLDQPGDPSAALVAIRPSTGRVVAMVGGRD
                     WSQSQVNLALGRKGGGSGRQPGSAFKPIAAAAALEAGIPFDAMYESSSASFTFDDGST
                     WSPTNSEGGGRGLMPLREALVHSVNGVYARLAMQLGPAAIVSQAKRMGVRSKLPVYPS
                     IVLGTAQVSVLDMAAAYATLANGGTMIEPTTIESVETPDGGMLRPDQDVDLGALSPGT
                     AYLVSRALEDVIRRGTGELADIGRPAAGKTGTTNDYVDAWFVGYTPDLVAAVWVGYPE
                     GAIPMYDVHGIRVMGGTFPALIWRQFMGAALAGEPPKPFRIPRAELVTVEIDPKSGLL
                     ATDWCPGKPKTMLRQLAPTQTCPVPTPAPVPSPSLSPDAEKEPKAGAEKDSKDKEEPE
                     ATAKPEPKPGRGREEEKDEGKDRS"
     gene            complement(5299..5667)
                     /locus_tag="VEV43_11320"
     CDS             complement(5299..5667)
                     /locus_tag="VEV43_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24155.1"
                     /translation="MVAWVAAGFAFAGTALTTVVQWGQLTDPYTGAASEILVRPVVSV
                     LRIGIVFTVYCGIGFLGSMLVAGKLPQKVGRSEVEFDEGPTYEAGAAESLAVRIRRLE
                     EAHRQLAQWFDEHERSHFDD"
     gene            complement(5768..>6824)
                     /locus_tag="VEV43_11325"
     CDS             complement(5768..>6824)
                     /locus_tag="VEV43_11325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYP24156.1"
                     /translation="RTPSRLELSYLQTAGDLTTMISQGEFPLPEERSDLADNLRVGDG
                     FVRVGIRGEVTYASPNAMSAIRRLGRVGDVVGRSLTEVMRELVHSSTAPVDSSVNAML
                     AGTQPGSFELGNDAVSLVLRALPLRAGGVATGGLILLRDVTELRSRERELMTKEATIR
                     EIHHRVKNNLQTIASLLRLQARRLSSDEAKAALTESVLRVGSIALVHETLSEEPSDVA
                     EFGEVARRIGRMVGEGLVLPERCVRIDVKGAAGPVGADLATPLAVVLTELLQNAVEHA
                     FPEGTDGTVTVELARTDGEVKMVVQDDGVGLDVAALEGSRLGLQIVRSLIDELGGTFA
                     IESAAGTRAEVRVRTRA"
BASE COUNT         1095 a         2352 c         2308 g         1069 t
ORIGIN      
        1 ggtcgcggcc ctcctggtga acgccgcggc ggggctcggc tatcgcgtct accggttccg
       61 caaagggggg ccgaagtcgg atgtgatcgg ccaggcgatc ctgggcgcgg tgctggtcgg
      121 gctggctgcg gggctggcgg cgggggccgg ctggccgcgc tggccggcgc tggtgtacgg
      181 cctgctgttc gggataatcg tgatgccgat atgggtgctg gcggtgctga tcccgctagg
      241 gccgcgcgcg atcgactacg cgttcaccgt cgtgtactgg gtgctgctgg ccgtcgtcgt
      301 ggtggccggt ctggccctct agaccgctca ggccccgttc ttgctcagca ggccccagtc
      361 cgccagacgc ctcgacagct cgtccgacgt cgtctggtag agcagcgcca gcgccatcag
      421 gtcgtcctct cggatcgtca ggacccggcc gttgaagtcg ccacgctgga tctgaatcgc
      481 ggtcgtgaag cgggacaggg ggtcgcggtc cgcctcgggg accttcgtga gcccctcgag
      541 gtccaggacg accctccggc gggtctcgac ctgcccgggc gcgggcggcg cgccgtcgtc
      601 gggaaggagc tcggataccg gcacgccgta gaactcggcg agctcggaga ggcgggccac
      661 ggtcacgttg cggtcgcccc gctcgtaggc ccccaccacc gcggccttcc acttgccgtc
      721 cgacgcctgc tgtacgtcct gcagggacat gcccttctgg accctgatcc gccttaggct
      781 ctcgcccagc gacttagcgt agtcaccgtt gctcaacgtc gttcctccgt ctcgaagagt
      841 agtcggggca cctgtcccgg ttgcattgtt cgacgcggag agtcatgacc ctccctatac
      901 ccactacttt ccgcattcag tctcgcacca gacgacgtga cttaggattg gcttgtgaaa
      961 accctgtgga aaggcgccat caccttcgga ctgatctcga tccccgtccg gctctactcc
     1021 gccgtccagg agaagagcct caagttccac ctgctccacg gcgaggacaa gggccgcatc
     1081 aagtacaagc gcgaatgcgc caagtgcggg aaagaggtcg gctgggacga catcatcaag
     1141 ggctacgagt tcgcgaagga ccagtacgtc acgttctccg aggaggagct cgacgccctc
     1201 gacgtcgact cgatcaaggc gatcgacgtc gtcgcgttcg tcccgctcga ggacatcgac
     1261 cccgtctact tcaacaagac ctattacgtc gggcccgagg cgacgggcgt caaggcgtac
     1321 cgcctcctcg ccgacgcgct cgaggccgag ggccaggtcg gcgtcgcgaa ggtcgcgctc
     1381 cgagactagg agcacctcgc cacgatccgc ctcaaaggcg atctgttcgt cctcgagacc
     1441 atgcactggc ccgacgagat ccgccagccc gacttcgagg agctcgacaa gaaggtcgag
     1501 gtccgcgaca ccgaggtcaa gatggcgcgc cagctgatcc agcagctctc gagcagctgg
     1561 gatcccgagg cgttccgcga cgagtaccgg gccgcgctgg aagagctcgt cgagaagaag
     1621 gtcgaaggtc aggagatcgc gatcgccgcc gagcccgagg aggagccgac gaaggtcgtc
     1681 gatctcatgg aggccctcaa ggcttcggtc gagcaagcca agaaggcgaa ggagtcgggg
     1741 gccggcaaga aggccgccaa gcccaaggct tcggcgggct aagcattgga cgccgctccc
     1801 gtggagaccg tccggatggt ggtcccggcc acgccgtcgt acctccaggt cctgcgactc
     1861 gtcgcggccg ggctcgcgtc gcggctcgcg ttctcgatcg acgagatcga ggacctcaag
     1921 atcgcggtcg acgagatgtc tgcgtacctc accggctctc agggacgcga cgggacgatc
     1981 gagatcgcct tcgaggtcga cacgtcctcg atcacgatcc ggggcaccgg caggttcgag
     2041 cccggccaca aggtgagggc cggcctcacg gacttctcga agatgatcct cgagaccgtc
     2101 gtcgactcgg cttcgctcga gcaatccgac ggcatcccga cgttcactct cgtcaagagc
     2161 aagcagctcg agacgacatg accgaacgcg ccaccctggg caaggagcag gtccgcgagc
     2221 tcttccgcgc ctaccgcacc gccggctcgc ccgacgagca ggccgaggcg aaggaccagc
     2281 tcgtcaacca gcacatcggt ttagtggagt tcctggcgcg ccgattccgt aacagaggag
     2341 agcctctgga ggatctggtg caggtgggca cgatcggttt gctgaaggcc atcgaacgct
     2401 tcgagctcga ccgcgaggtc gagttctcga cgtacgcgac gccgacgatc gtcggcgagc
     2461 tcaagcgaca tttccgcgac aagggctggg ccgtccgcgt cccccgccgg ctgcaggagc
     2521 tccacctcga gctcaccaag gtcgtcgggc acctcggcca cgacctcgga cgctctccca
     2581 ccgtcgcgga gatcgcaaag gccgccggca ccaccgaaga ggcggtgctc gaagggctcg
     2641 aggtcgcgca ggcctacaac ttcacgtcgc tcgacgcgcc gatcgacacc gacgacgggg
     2701 gctcgacgac gttcgccgac cagctgggcg cggacgacga gcacctcgag aacctcgagt
     2761 accgcgcgtc gctggcgccc gagatggcga agctccccga acgcgagcgc cgcatcctgt
     2821 acctccgctt cttcaagggg atgacgcagt ccgagatcgc cgaccggctc ggcatctcgc
     2881 agatgcacgt ctcccgcctc ctcaaccgaa cgctcggaca gctgcgcgag gcgttagagg
     2941 acacctgatg cgcgcactgc gcatcgccgc gctcggcacc gtcgtcgcgc tcgtcgccgg
     3001 cggctgctcg ctgcggccgg tcgacctcga cgagcagaag ccgctggcgc ttcgctccac
     3061 gttccgcgcc gccgacggca gcgtgctcgc acgcttcttc aaggaaaacc gcaagctcgt
     3121 cgagctcgac gacctgccgc ggtcgttgat cgacgcggtc gtcgctgccg aggacgcgcg
     3181 cttcttcgag cacccggggt tcgacgtgcg ggcgatcgct cgcgctgcgc tcgtcaacct
     3241 cgcggagggc gagaccgttc agggcgggag cacgatcacg cagcagtacg tgaagaacac
     3301 gttcttccgg aagccggcga agacgctcga gcgcaaggcc aaggagctgc ggctcgcgat
     3361 cgaggtcgag aagcgctacg acaagcgcga gatcctcgag ctctacctca acacggtcta
     3421 cttcggccgc ggcgcgtacg gcgtggcgac cgcgtccgag agctacttcg ggcacggggt
     3481 gcgcgacctg acgctgtcgg agtccgcgct gctcgccgca gtcatccgcg caccgtctcg
     3541 ctacgacccg cgcgagcatc cgcggcgcgc tctggggcgg cgggactacg tgctcgaccg
     3601 catggagacg ctggggaagg tgactccccg gcgtgccgcg gcggcacggc gccggcctct
     3661 gggcgtggtc ccggtctccg ctccgcccga ggcccgctac ccgtacttcg tcgaggccgg
     3721 ccggcaggag ctgctggccg actcgcggct ggggaggtcc gacgacgagc gggctcgcgc
     3781 gatctacgag ggcgggctcg tagtgaagac gacgctcgac ccccagatgc aggccgccgc
     3841 cgagcgcgcc gtagcggacg tgctcgacca gcccggcgat ccttcggccg cccttgtagc
     3901 gatacggccg tcgacggggc gggtggtcgc gatggtcggg ggccgggact ggtcacagtc
     3961 gcaggtgaac ctcgcgctcg ggcgcaaggg cggcgggtcg gggcggcaac cgggctctgc
     4021 cttcaagccg atcgccgccg cagccgcgct cgaggccggg atcccgttcg acgcgatgta
     4081 cgagtcgtct tctgcttcgt tcacgttcga cgacgggtcg acgtggagcc cgacgaattc
     4141 cgaaggcggg ggccgcgggt tgatgccgct gcgcgaggcc ctcgtccatt cggtcaacgg
     4201 cgtgtacgcg cggcttgcga tgcagctcgg ccccgccgcg atcgtgtctc aggcgaagcg
     4261 gatgggggtc cggtcgaagc tgcccgtcta tccgtcgatc gtgctcggga ccgcgcaggt
     4321 gagcgtgctc gacatggcgg ccgcgtacgc gacgctcgcc aacggcggga cgatgatcga
     4381 gccgacgacg atcgagtcgg tcgagacgcc ggacggaggc atgctccggc ccgaccaaga
     4441 cgtggacctc ggcgcgctgt cgccggggac ggcgtatctc gtcagccgtg cgctggagga
     4501 cgtgatccgg cgcgggaccg gagagctcgc cgacatcggc cgtcccgcgg cgggcaagac
     4561 cgggaccacg aacgactacg tcgacgcgtg gttcgtcggt tacacgcctg atctcgtcgc
     4621 ggcggtatgg gtcggatacc ccgagggtgc gatcccgatg tacgacgtgc acggcatcag
     4681 ggtcatggga gggacgttcc cggcgctgat ctggcgtcag ttcatggggg ccgcgctcgc
     4741 gggcgagccc ccgaagccgt tccgcatccc gagggccgag ctggtcacgg tggagatcga
     4801 cccgaagtcg ggcctgctgg cgacggactg gtgccccggc aagccgaaga cgatgctgcg
     4861 ccagctggcg ccgacgcaga cctgcccggt cccgaccccg gcccccgtac cgtcgccgtc
     4921 actgtcgccg gacgccgaga aagagccgaa ggcgggggcc gagaaggact cgaaggataa
     4981 ggaggaaccc gaggcgacgg cgaagccgga gccgaagccc ggccggggtc gagaagaaga
     5041 gaaggacgaa gggaaagacc gaagctgagg tctctcggtt agcccaacct atttgcgagg
     5101 aggtcggccg agagccttct ccaagtacat ctcggtcgtc ttgcgctcgg cgatcgcctc
     5161 gtcgatcaac tcccgtgctc tccttagatg ttctgcggct ttgtcgctct tcttggggtt
     5221 gggtcgttca ggttggttgg tcacgacatc ctccgtagct cgcgaaccat taggtctcga
     5281 tgataagggt gggtcggatc agtcatcgaa atgtgatcgc tcgtgctcat cgaaccactg
     5341 ggctagttgc cggtgtgctt cttccaacct gcggatgcgg acggcgagcg actccgctgc
     5401 tcccgcctcg tatgtcggcc cctcgtcgaa ctcgacctcg ctccttccta ccttttgcgg
     5461 cagcttgccg gccacgagca tcgaaccgag gaagccgatg ccgcagtaca cggtgaacac
     5521 gatgccgatg cgaagaacgc tcaccacggg acggacgagg atctcggatg ccgccccggt
     5581 gtacgggtcc gtaagctggc cccactgcac gaccgtcgtc agggccgtcc ccgcgaaggc
     5641 gaaacctgcg gccacccacg cgaccaattg tctgaacgtc ccatccgatg cggagggccg
     5701 gcgaattgtc atcgccccat cgtgcggccc tatcgccacc gcacgtgtgg cgcggaccac
     5761 gctccggcta ggccctcgtt ctcacccgca cctcggctct cgttccggcg gccgattcga
     5821 tcgcgaacgt tccgcccagc tcgtctatca gcgagcgcac gatctgcagg cccaggcggg
     5881 agccctccag cgccgctacg tcgaggccca cgccgtcgtc ctgcacgacc atcttgacct
     5941 cgccgtcggt gcgcgccagc tcgacggtga ccgtcccgtc ggtcccctcc gggaacgcgt
     6001 gctcgaccgc gttctggagc agctccgtca gcacgaccgc cagcggcgtc gccaggtcgg
     6061 ctcccacggg ccccgccgcc cccttcacgt ctatccgcac gcaccgctcg ggcaacacca
     6121 agccctcgcc gaccatgcgt ccgatccggc gtgcgacctc gccgaactcg gcgacgtcgc
     6181 tgggctcctc cgacagcgtc tcgtgtacga gcgcgatcga cccgacgcgc agcacactct
     6241 cggtcagcgc ggccttcgcc tcgtccgagc tcagccgccg cgcctggaga cgcagcaagc
     6301 tcgcgatcgt ctggaggttg ttcttcacgc gatggtggat ctcgcggatc gtcgcctcct
     6361 tggtcatcag ctcccgctca cggctgcgca gttcggtgac gtcgcgcagc aggatcagcc
     6421 cgccggtggc cactcccccc gcgcgcagcg gcaacgcccg gagcaccaag gacaccgcgt
     6481 cgttgcccag ctcgaaggag ccgggctggg tgcccgccag catggcgttg accgagctgt
     6541 ccaccggcgc ggtcgaggag tggacgagct cgcgcatcac ctccgtcagg gaccggccga
     6601 ccacgtcgcc gacccggccg agccggcgaa tggccgacat ggcgttgggg ctggcgtacg
     6661 tgacctcgcc gcggatgccc acgcgtacga acccgtcgcc gacacgcagg ttgtcggcga
     6721 ggtcggagcg ctcctccggc agcgggaact cgccctggct gatcatggtg gtgaggtcgc
     6781 cggcggtctg caggtaggac agctccagcc ggctcggcgt gcgc
//
LOCUS       DASQDO010000406         5022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_104039, whole genome shotgun sequence.
ACCESSION   DASQDO010000406 DASQDO010000000
VERSION     DASQDO010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104039"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VEV43_11330"
     CDS             complement(<1..1170)
                     /locus_tag="VEV43_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP24157.1"
                     /translation="MTKDEQAIFMTATEGVDTDRISHHLHYEMPGTGERRGHVNWAVG
                     VNRTPDPEPEAREQSFRHVYLPPLRDARRELSSGATDRLRFLLEVLAEPGEVDQLQTE
                     ADEAFRELEKHPLIKRADSKVRKQLSELTHGTIRQRAQLGFVEAELRRLSAALRMKLG
                     EWGLDVQELVSSGHGYANLLFLATVIVELQAARDADLTLFLVEEPEAHLHPQLQGAVL
                     DFLREVSQPRDEGGQIQVVVVTHSAQLTASVPCEHVEVLRTVSRTITKEGEAPSTYRE
                     SSPVPVWELGIAGTQLAKINRYLDVTRAALLFAPRVILVEGIAEALLMPGIAKRLLSE
                     EDLARFRASSLIAIEGVDFEPYVRLLLTPFEGTRIADRVLVVTDADPEAPGDRAAT"
     gene            complement(1886..1959)
                     /locus_tag="VEV43_11335"
     tRNA            complement(1886..1959)
                     /locus_tag="VEV43_11335"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1922..1924),aa:Glu,seq:ttc)
     gene            2009..2782
                     /locus_tag="VEV43_11340"
     CDS             2009..2782
                     /locus_tag="VEV43_11340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP24158.1"
                     /translation="MTVDPAEAIQGNRQAWDRGSAEYQRQHSDFLTEKALAWGIWRVP
                     EERLRLLGDVDGKVVLEYGCGGAQFATALTGQGAHAIGLDISANQLRHAQEHMSRQGQ
                     TVPLVQASAHMTPFRDASFDVVFSDYGATTFVDPYLTVPEAARVLKPGGIFVFAATSP
                     VYIMCIDPKTDELTTSLQTPYFGMRTLEDDEGFRDYQVTHGEWITLFRKNRLVVEELI
                     ETQPDEGVTTTYAGRPPEWARRWPAENIWKLRKEPQREV"
     gene            2785..3090
                     /locus_tag="VEV43_11345"
     CDS             2785..3090
                     /locus_tag="VEV43_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24159.1"
                     /translation="MADEDQVTTPVDERDDVDHSEFTSDSNECPECGEPVDNLRKTCI
                     KCGYEYGKDDYDDTEAGNELRAGAALDDEGNEITDEDELDEETADDGETAEDGEQEG"
     gene            3087..3515
                     /locus_tag="VEV43_11350"
     CDS             3087..3515
                     /locus_tag="VEV43_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYP24160.1"
                     /translation="MTDPARKILETYKTFAVVGCSSDPWRASHGVAQFLQSRGYRVIP
                     VTPFENEILGERCYPDLASIPEEEGVEVADIFRRPDAAGAHVDEAIEVGLKAVWMQIG
                     VIDDAAAERARAAGLDVVMDRCPKIEYPRVFAGRASTPAS"
     gene            3549..3851
                     /locus_tag="VEV43_11355"
     CDS             3549..3851
                     /locus_tag="VEV43_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYP24161.1"
                     /translation="MEAYDLAFDALGDRTRRRTFELLKGRPQSVGALAEQLPVSRPAV
                     SQHLRVLKDAGLVRDEQVGTRRLYSVDPGSIEALRAYFDDMWTDALARFKERADSP"
     gene            3848..4285
                     /locus_tag="VEV43_11360"
     CDS             3848..4285
                     /locus_tag="VEV43_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYP24162.1"
                     /translation="MTPLVKELTVAAPVERAWEVFTDEITSWWPIPTHSVEPDRVSEI
                     VFEARPGGRILERRHDGTECSWGEVDLCEPPHRVRFSWQPNHDRPAATEVEVTFTATP
                     DGTRVRLEHRGWERLGAQEGAESRAEYDPGWDFVLARYESTIT"
     gene            complement(4278..>5022)
                     /locus_tag="VEV43_11365"
     CDS             complement(4278..>5022)
                     /locus_tag="VEV43_11365"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HYP24163.1"
                     /translation="GEPPPFCGGLHGHGVVGRDGAIFLPKESCEQPWLGISRDEGLTW
                     EIVRVSNLDAGPGLLDPSVDVDDKGNVYYAWTGGDRRVYLTTSKDGGRTWRPATVVSP
                     PGVHEVNLATLDASGVGKVAIAYMGSASSPWRRDCREKESCPETADYAKTTWNGYVTT
                     TTNALSGNPKLVSTAVNPPKDPIYRGRCGPGRCGLVFDFIDVVIGPSGDAFATFVDAC
                     VAQCTNKTSVANYGSDGVVARIVGGPTLK"
BASE COUNT          888 a         1670 c         1629 g          835 t
ORIGIN      
        1 cgtcgcggcc ctatctcctg gagcctctgg gtcggcgtcc gtgacgacca gaacgcggtc
       61 tgcgatccgg gtgccctcga agggcgtgag caggagacgg acatacggtt cgaagtcaac
      121 gccttcgatc gctatcaggg agctcgctct gaatcgcgca agatcttcct cggagagtag
      181 ccgctttgcg atacccggca tgaggagagc ttcagcgatt ccttctacga ggatcactct
      241 tggcgcgaag agaagcgcag cccgagtgac gtccaaataa cgattgatct ttgccaactg
      301 ggtgcccgcg attcccagct cccaaacggg cacggggctc gactcccgat aggtgctcgg
      361 tgcctcgccc tccttagtga tagtcctgct gacggttctc aggacctcaa catgttcgca
      421 aggaaccgag gctgtcaatt gagcggaatg cgtcacaaca acaacctgaa tctgtccgcc
      481 ttcatctcgc ggctgcgaga cctctcgtag gaaatcgaga actgctccct ggagttgggg
      541 atgaagatgt gcctctggct cctccacaag aaacaaggtg aggtcagcgt cgcgcgcggc
      601 ttgcagctcc actatgacgg tcgcgaggaa caataggttg gcgtaaccgt gaccggatga
      661 aacgagctct tggacatcaa gcccccactc gccgagtttc atccgtagcg ccgcggacag
      721 gcgccgtagc tccgcttcca cgaaacccag ctgagcgcgt tgacgaatcg ttccgtgggt
      781 gagttcgctc aactgcttcc ggaccttgct gtccgccctc ttgatcagcg gatgcttctc
      841 cagctcgcgg aacgcctcat cagcttcggt ttgcagttgg tcgacctcgc cgggctcggc
      901 gagcacttcc agcagaaagc gcaaccggtc ggtcgcccca gaactcaact cgcgccgcgc
      961 gtcgcgtagc ggaggtagat agacgtggcg gaagctttgc tctcttgctt cgggttcggg
     1021 gtcaggcgtt ctgttaaccc ctactgccca gttgacgtgg cctctgcgtt ctcccgtccc
     1081 gggcatctcg taatgaaggt gatgggagat gcgatccgtg tcaacgcctt ccgttgcggt
     1141 catgaagatt gcctgctcgt ccttcgtcaa ccctccgtag tacacgctca ggtcaaaact
     1201 ccgtctggag cgtcgcggtt gacatcctgg ggctcgcaat accgagttcg gcgaccatcc
     1261 gcaggaagag tgagaagacg aatggcttca atgaggttcg acttaccact gttgttttct
     1321 ccgactacga ccgtaagatc cttcttgaac gccacttccg cctcccgaaa tgaacggaaa
     1381 ttgcgggcga caaggtggcc gaggtacatc gttttagtac accacggcct tgggaagctg
     1441 gagagctagg cggcgatgca aggcgtgaga cggcgaatgc ctctgtcgtt agccctcgtt
     1501 gcaccggatg ccgggccggt ggtctggaac gttcccgttg cctgtaagcg cgcaatgcag
     1561 atctcacgat cccgtcgccg ctgatccacg tcacgtacaa gcgagagttc cgggcctcgg
     1621 cccgaaggtg ccctgcgacg cttcgttgtt aggcctttgc ctatacaggc cgccgcgatt
     1681 ttcgccgtgg gcttgcacgc gttcgttgcg gcgagcggct tgaagaggag cgcccttcca
     1741 ggactcggcc agccttccgt accgccagag agggagtcga acgcccccgc gcgtcgacat
     1801 gcgcgcgggc aacaggtggg caacaggaat ccgagcggaa tgcaagtgaa gcggcgaagt
     1861 agcctctgac ctgcaccttt actcgtggcc ccaacgggat tcgaacccgt gctaccgcct
     1921 tgaaagggcg gcgtcctgac caggctagac gatggggccc cgcccctcgc ggggctcgac
     1981 gatgctacgt caccgctacg ctgcccccat gaccgtcgat cccgccgagg ctatccaggg
     2041 caaccgccaa gcctgggacc ggggctccgc cgagtatcag cgccagcatt ccgacttcct
     2101 caccgagaag gccctcgcct gggggatctg gcgcgtgccc gaggagcgac tccggctcct
     2161 cggcgacgtc gacggcaagg tcgtcctcga gtacggctgc ggcggggccc agttcgccac
     2221 cgccctcacg ggacagggcg cgcacgccat cggcctcgac atctccgcga accagctcag
     2281 gcatgcgcaa gaacacatga gccggcaggg acagacggtc ccactcgtcc aggcctcggc
     2341 ccacatgacg ccgttccgcg acgccagctt cgacgtcgtc ttctccgact acggcgcgac
     2401 cacgttcgtc gatccctatc tcaccgtccc cgaagcagcg cgcgtcctca agccgggcgg
     2461 catcttcgtc ttcgccgcga cgagccccgt ctacatcatg tgcatcgacc cgaagacgga
     2521 cgagctcacg acttcgctcc agacgccgta cttcgggatg cgcacgctcg aggacgacga
     2581 gggcttccgc gactaccagg tcacgcacgg cgagtggatc acgctgttcc gcaagaaccg
     2641 gctcgtcgtc gaggagctga tcgagacgca gcccgacgag ggagtaacga cgacgtacgc
     2701 gggtaggccc ccggaatggg cccgccgctg gccggccgag aacatctgga agctgcgcaa
     2761 agagccgcaa cgggaggtct agacgtggcc gacgaagatc aggtgacgac tccggtcgac
     2821 gagcgcgacg acgtcgacca ctccgagttc acttccgact cgaacgagtg ccccgagtgc
     2881 ggtgagcccg tcgacaacct ccgcaagacc tgcatcaagt gcggctacga gtacggcaag
     2941 gacgactacg acgacaccga ggccggcaac gagctgcgcg cgggcgcggc cctcgacgac
     3001 gagggcaacg agatcaccga cgaggacgag ctcgacgaag agacggcaga cgacggagag
     3061 acggccgagg acggggagca agaaggatga cggacccggc ccgcaagatc ctcgagacct
     3121 acaagacgtt cgccgtcgtc ggctgctcgt ccgacccctg gcgcgcgagc cacggcgtcg
     3181 cccagttcct gcagagccgc ggctaccgcg tcatcccggt cacgcccttc gagaacgaga
     3241 tcctcggcga gcgttgctat cccgacctcg cgtcgatccc ggaggaggag ggcgtcgagg
     3301 tcgcggacat cttccggagg cccgacgccg ccggagccca cgtcgacgag gcgatcgagg
     3361 tcggcctgaa ggcagtgtgg atgcagatcg gcgtgatcga cgacgctgcg gccgagcggg
     3421 cccgggccgc cgggctcgac gtcgtcatgg accggtgccc caagatcgag tacccgcggg
     3481 tcttcgccgg gagggcgtcc acgccggcct cttgacgaga ggcatccgta agtcatagct
     3541 tacggtaagt ggaagcttac gatcttgcct tcgacgcact cggcgaccgg acccgccgcc
     3601 ggaccttcga gctcctgaag gggcggccgc agtccgtcgg cgccctcgcc gagcagctcc
     3661 cggtgagcag gccggcggtc tcgcagcacc tccgcgtcct caaggacgcc ggtctcgtgc
     3721 gcgacgagca ggtcgggacc aggcgcctgt acagcgtcga cccgggctcg atcgaggcgc
     3781 tgcgcgctta cttcgacgac atgtggaccg acgcgctcgc gcgcttcaag gaaagggccg
     3841 attcgccatg acgcctctgg tcaaggagct gacggtcgcc gcgccggtcg agcgagcgtg
     3901 ggaggtgttc accgacgaga tcacgtcgtg gtggccgata ccgacgcact cggtcgagcc
     3961 ggatcgagtc agcgagatcg tcttcgaggc gcggcccggc gggcggatcc tcgagcgacg
     4021 gcacgacggc accgaatgct cgtggggcga ggtcgacctc tgcgagcctc cccatcgggt
     4081 gcggttctcg tggcagccga accacgatcg tcccgcggcg accgaggtcg aggtgacgtt
     4141 cacggcgacg ccggacggga cccgcgtcag gctcgagcac cgcggctggg aacgcctagg
     4201 cgcacaggag ggcgcggagt cgcgcgccga gtacgacccg ggctgggact tcgtgctcgc
     4261 ccgttacgag agcacgatta cttgagcgtc ggccctccga cgatccgcgc cacgactccg
     4321 tccgagccgt agttggcgac ggacgtcttg ttcgtgcact gcgcgacgca cgcgtcgacg
     4381 aacgtcgcga acgcgtctcc gctcggcccg atgacgacgt cgatgaagtc gaagacgagc
     4441 ccgcaccggc caggcccgca acgtccgcga tagatcgggt ccttcggcgg gttgaccgcg
     4501 gtcgacacga gcttggggtt gcccgagagc gcgttcgtcg tcgtcgtgac gtagccgttc
     4561 cacgtcgtct tcgcgtagtc cgcggtctcg gggcacgact ccttctcgcg gcagtcgcga
     4621 cgccacggcg agctcgcact gcccatgtac gcgatcgcga ccttgccgac tccgctcgcg
     4681 tcgagcgtcg cgaggttgac ctcgtggacg cccggcgggc tcacgacggt cgcggggcgc
     4741 cacgtccggc ccccgtcctt cgaagtcgtg aggtagacgc ggcggtcacc acccgtccac
     4801 gcgtagtaga cgttgccctt gtcgtcgacg tcgacgctcg ggtcgagcag cccggggccg
     4861 gcgtcgaggt tggagacgcg cacgatctcc cacgtcagtc cctcgtcgcg gctgatgccc
     4921 agccacggct gctcgcagct ctccttgggc aggaagatcg ctccgtcgcg gccgacgact
     4981 ccgtggccgt ggagcccgcc gcagaaggga ggcggctcgc cg
//
LOCUS       DASQDO010000407         5343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_348316, whole genome shotgun sequence.
ACCESSION   DASQDO010000407 DASQDO010000000
VERSION     DASQDO010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348316"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="VEV43_11370"
     CDS             complement(<1..1582)
                     /locus_tag="VEV43_11370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HYP24164.1"
                     /translation="MTTRVVFLGGLGEVGRNMFAVEASDRIVVVDCGLSFPNEEMLGV
                     DLVLPDFTWICERADKCEAVVLTHGHEDHVGALSWFLRDVPVPVYGTPLTLGVARRRL
                     DEFGVDAELREISEGTREIGPFRFTFLAMSHSIPDAVGLAVDTPDGRILYTGDFKLDE
                     TPVDGRTTDLATLRRLGDEGVDVLLSDSTNAETPGRTPSEAVVGPVLSRIFDEARGRI
                     VVACFASNLHRIQQVCEVAERFDRKVAFVGRSMIANAEVGRELGHLQVRPDTIVPIED
                     AHRLPRERAVVLCTGSQGEPLSALSLIAAGEHKAVHLDAGDTVILSASPIPGNESAVH
                     RVLNGIYKAGAEVFHSGTAAVHVSGHAASDELADMIRTVRPRHFVPVHGEYRQLALHS
                     KIAQANGVPEDRITIVEDGDVIELDDGRVRRGDAVRSGMILVDGLGVGDVGPVVLRDR
                     RVLAGDGVIICVVTIDGQTGEVLAGPDLVSRGFVYEEESREFLDEAADRIADALEQLE
                     RDRVTDWSTIKKACRHALG"
     gene            complement(1579..2481)
                     /gene="dapA"
                     /locus_tag="VEV43_11375"
     CDS             complement(1579..2481)
                     /gene="dapA"
                     /locus_tag="VEV43_11375"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HYP24165.1"
                     /translation="MSAARFGQLITAMVTPFASDGALDPKAARRLAGHLVDHGSDGLV
                     VAGTTGESPTLSHAEKLELFEAVLAEVGDRATVIAGTGSYDTRETIELTAAACDLGVH
                     ACLVVTPYYSKPPQNGLLAHFRAVADAATKPLMLYDIPGRTARRIERETMVELAAHER
                     IVAVKDAVGDAGETAALRRDLDAAGHEDFEIYSGDDPLVFPHLAAGAVGLVSVCSHLV
                     GPQLKQVFESWDAGRTADARRSYLELLPFMRTIMTLTASPIPVKAAVNMLGLDVGEPR
                     LPLVPATGDESAAIRAAMEGQGLL"
     gene            complement(2519..3019)
                     /locus_tag="VEV43_11380"
     CDS             complement(2519..3019)
                     /locus_tag="VEV43_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24166.1"
                     /translation="MRRLAAVALALALLPACFGPDPNVTEEGKGKPLLTIEFPETVSR
                     GSVETATLTIDNPGPGDMGSVVVAFATAGVAGASGPLPAELVPLTSSSENPMIESITP
                     EPREVSDDGVLYVFDGLEEGASREISFDIRVPEEPGPAANSVSVYAGEDADRIKGLLL
                     RTVVTG"
     gene            complement(3020..3754)
                     /gene="dapB"
                     /locus_tag="VEV43_11385"
     CDS             complement(3020..3754)
                     /gene="dapB"
                     /locus_tag="VEV43_11385"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HYP24167.1"
                     /translation="MIRVGVLGAGGRMGAETCRAVDADPELELVPRVGRHDPLETLVS
                     ARAEVAVEFTTPDSVKENVRFCLSHGIHCVVGATGLGDDDLAEVREWCREGSVNALVA
                     PNFALGAVLMMSFAAAAAKHFDTAEIVERHHERKLDAPSGTALRTARLMNEARGGRAW
                     ASRRGDESVPGSRGGDASGVHVHSLRVPGSVAHQEVVLGAAGETLTIRHDSLDRTSFM
                     PGVVLAVKQVSRLDGLVVGLENLLDL"
     gene            complement(3762..5018)
                     /locus_tag="VEV43_11390"
     CDS             complement(3762..5018)
                     /locus_tag="VEV43_11390"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYP24168.1"
                     /translation="MTKFARTELDSGITVVTESMTEVRSISVGFWFDVGARDEPDELA
                     GTSHFLEHLLVKGPGSRSARQIAESFDAVGGDVNAFTGKEYTCYYARVLDDDLPMAVD
                     VLSDMILNSVIDPTELESERNVILEEIAMHADAPDELVHDLFFRSMWDGHPLGRPVLG
                     YNETIGRVARDQVAGYWRDRYGPCNLVVAAAGRVDHDDLVRRVDSIFSPAGGIKTSRT
                     GEAPAARSGVNVFKRPTEQAHIMIGTEALRRGHPDRHALTLLDSVLGSGMSSRLFQEV
                     REKRGLAYSVYSYRSMFADTGTFSVYAGTLPQNAETVMEIVSNEIESVVRAGITDAEL
                     ARAKGNVKGSLVLSSEDPGSRMNRLGRQQVTTGEILSIDELIARFEVLTMDDVRRVAR
                     DVLEDARFSITVVGPFEEDAFDRYAA"
     gene            complement(5018..>5343)
                     /locus_tag="VEV43_11395"
     CDS             complement(5018..>5343)
                     /locus_tag="VEV43_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24169.1"
                     /translation="NVGDQIEVEITDIDRQGRINLTPIAWLERQVEAGKTIEEARAAA
                     VAGGGGGGGGDRGGRDRDRGGRDRDRGGRVRGGLDRGPWDGGGGGDRGPRRERAPRRP
                     DEDSL"
BASE COUNT          773 a         1891 c         1904 g          775 t
ORIGIN      
        1 cgcccagcgc gtggcggcac gccttcttga tcgtcgacca gtccgtgacc cggtcccgct
       61 cgagctgctc gagcgcgtcg gcgatgcggt cggcggcctc gtccaggaac tcgcgcgact
      121 cctcttcgta gacgaagccg cggctcacga ggtcgggccc ggccaggacc tcgccggtct
      181 gcccgtcgat cgtgaccacg cagatgatca cgccgtctcc ggcgaggacg cgccggtcgc
      241 gcagaacgac ggggcccacg tcgccgacgc cgaggccgtc gacgaggatc atcccggagc
      301 gcacggcgtc gccgcggcgg acccggccgt cgtcgagctc gatcacgtcg ccgtcctcga
      361 cgatcgttat gcggtcctcg gggaccccgt tcgcctgcgc gatcttcgag tggagcgcga
      421 gctgccggta ctcgccgtgg accgggacga agtgccgggg ccggaccgtg cggatcatgt
      481 cggcgagctc gtcggaggcc gcgtggcccg agacgtggac ggccgcggtg cccgagtgga
      541 agacctcggc ccccgccttg tagatgccgt tcagcacccg atggaccgcg gactcgttgc
      601 cggggatggg gctcgccgag aggatcaccg tgtcgccggc gtcgaggtgg acggccttgt
      661 gctcgcccgc ggcgatcagc gagagcgccg acagcggctc gccctgcgag cccgtgcaca
      721 gcacgaccgc cctctcgcgc ggcagccggt gggcgtcctc gatcggcacg atcgtgtccg
      781 gccgcacctg gaggtggccg agctcgcgcc cgacctcggc gttcgcgatc atcgagcgtc
      841 cgacgaacgc gaccttccgg tcgaagcgct ccgcgacttc gcaaacctgc tggatgcggt
      901 ggaggttcga ggcgaagcac gcgacgacga tgcggccgcg cgcttcatcg aagatgcgac
      961 tgagcacggg cccgacgacg gcttccgaag gagtgcgccc gggcgtctcg gcgttcgtcg
     1021 agtccgacag caggacgtcg acgccctcgt cgcccaggcg ccgcaacgtc gccaggtcgg
     1081 tcgtgcgccc gtcgaccggc gtctcgtcga gcttgaagtc gcccgtgtag aggatccggc
     1141 cgtccggggt gtcgacggca agcccgacgg cgtcggggat cgaatgggac atggcgagga
     1201 acgtgaagcg gaacggtccg atctcgcggg tgccttcgga gatctcgcgc agctccgcgt
     1261 cgacgccgaa ctcgtcgagc cgccgccggg ccacgccgag cgtcagtggt gtcccgtaga
     1321 cggggaccgg gacgtcgcgc aggaaccacg acagcgcgcc gacgtggtcc tcgtgcccgt
     1381 gcgtcagcac gactgcctcg cacttgtccg cgcgctcgca gatccacgtg aagtccggca
     1441 gcacgaggtc gacgccgagc atctcctcgt tcgggaacga gagcccgcag tcgacgacga
     1501 cgatccggtc cgaggcctcg accgcgaaca tgttccggcc gacctcgccg aggccgccga
     1561 ggaagacgac tcgggtcgtc acaggaggcc ttggccctcc atggcggcgc ggatcgcggc
     1621 actctcgtcg ccggtcgcgg ggacgagcgg gagccggggc tcgcccacgt cgaggccgag
     1681 catgttcacg gcggccttca ccgggatcgg gctcgcggtg agcgtcatga tcgtccgcat
     1741 gaacgggagc agctcgaggt aggagcgacg ggcgtcggcg gtccggcccg cgtcccacga
     1801 ctcgaacacc tgcttcagct gcgggccgac gagatgcgag cacaccgaca ccaggccgac
     1861 tgcgccggcc gcgagatgcg ggaacacgag cgggtcgtcg ccggagtaga tctcgaagtc
     1921 ctcgtgcccg gccgcgtcga ggtcgcgtcg caacgctgcc gtctcgcccg cgtcgcccac
     1981 tgcgtccttc accgcgacga tgcgctcgtg ggccgcgagc tcgaccatcg tctcgcgctc
     2041 gatgcggcgg gcggtgcggc ccgggatgtc gtagagcatc agcggcttgg tcgccgcgtc
     2101 cgcgaccgct cggaagtgcg cgagcagacc gttctgcggg ggcttcgagt agtagggcgt
     2161 gacgacgagg cacgcgtgga cgccgaggtc gcaggccgcg gccgtcaact cgatcgtctc
     2221 gcgggtgtcg tagctgccgg tgccggcgat caccgtcgcc cggtccccca cctccgctag
     2281 gaccgcctcg aacagctcga gcttctcggc gtggctcagc gttggcgact cgccggtcgt
     2341 gccggcgacg acgagaccgt cggagccgtg gtcgacgagg tggccggcga ggcggcgcgc
     2401 cgccttcggg tcgagcgccc cgtcggaagc aaagggcgtg accatggccg tgatgagctg
     2461 accgaagcga gcggcggaca tcggggggcc tcctctttcg atactcgtag cggtcacgct
     2521 aacccgtcac caccgtccgg agcagcaatc ccttgatgcg gtcggcgtcc tcaccggcgt
     2581 agacgctgac ggagttcgca gcggggcccg gctcttccgg gacccgtatg tcgaacgaga
     2641 tctcgcgcga ggcgccctcc tccaggccgt cgaagacgta gaggaccccg tcgtcggaga
     2701 cctcgcgcgg ctcgggcgtg atcgattcga tcatcgggtt ctcggacgag ctggtgagcg
     2761 gcacgagctc ggccgggagc ggcccagacg cccctgcgac gccggcggtg gcgaacgcga
     2821 cgacgaccga gcccatgtcg cccggcccgg ggttgtcgat ggtcagcgtg gccgtctcga
     2881 cggagcctcg cgacacggtc tcggggaact cgatcgtcaa cagcggcttc cccttgccct
     2941 cctcggtcac gttggggtcc ggcccgaagc acgcaggcag gagcgcgaga gcgagggcca
     3001 ccgccgccag ccgtcgcatc tagaggtcga gaaggttctc gagcccgacg acgaggccgt
     3061 cgagccggga aacctgcttc accgcgagga cgacgcccgg catgaacgac gtgcggtcga
     3121 gcgagtcgtg gcggatcgtg agggtctcgc cggccgcgcc gaggacgacc tcctggtgcg
     3181 cgaccgatcc cggcacccgc agggagtgga cgtggacacc ggacgcgtcc ccgcccctgc
     3241 tcccggggac cgactcgtcc ccgcggcgcg aggcccaggc ccggcccccg cgcgcttcgt
     3301 tcatgaggcg cgccgtgcgc agcgcggtcc cggacggggc gtcgagcttg cgctcgtggt
     3361 gccgctcgac gatctcggcg gtgtcgaagt gcttcgccgc ggccgccgcg aacgacatca
     3421 tcagcacggc tcccagcgcg aagttgggcg cgaccagagc gttgaccgaa ccctcacggc
     3481 accactcgcg gacctcggcc aggtcgtcgt cacccagccc ggtcgccccg acgacgcagt
     3541 ggatgccgtg gctgaggcag aagcggacgt tctccttgac ggagtcgggc gtcgtgaact
     3601 cgacggccac ctcggcacgg gccgagacga gcgtctcgag cgggtcgtgg cgtccgacgc
     3661 gcgggacgag ctcgagctcg gggtcggcgt cgaccgcacg gcacgtctcc gcgcccatgc
     3721 ggcccccggc ccccaagacc ccgacgcgga tcatcggacg atcaggcggc gtagcggtcg
     3781 aacgcgtcct cttcgaacgg ccccacgacc gtgatcgaga agcgggcgtc ctctagcacg
     3841 tcgcgcgcga cccggcgcac gtcgtccatc gtcagcacct cgaagcgagc gatgagctcg
     3901 tcgatcgaga ggatctcgcc ggtcgtgacc tgctggcggc cgagacggtt catgcggctg
     3961 cccgggtcct ccgacgacag cacgagcgag cccttgacgt tgcccttggc gcgggcgagc
     4021 tcggcgtcgg tgatcccggc gcggacgacc gactcgatct cgttcgagac gatctccatc
     4081 acggtctcgg cgttctgcgg aagggtcccc gcgtagaccg agaacgtccc ggtgtcggcg
     4141 aacatcgagc ggtaggaata gaccgagtat gcgaggccgc gcttctcgcg cacctcctgg
     4201 aacaggcgcg acgacatccc gctgccgagc acgctgtcga gcaacgtcag cgcgtgccgg
     4261 tcgggatggc ccctccggag cgcctcggtc ccgatcatga tgtgagcctg ttcggtcggg
     4321 cgcttgaaga cgtttacgcc cgagcgcgcc gcgggggcct cgccggtgcg ggacgtcttg
     4381 atcccgcccg ccggcgagaa gatcgagtcg accctgcgca cgaggtcgtc gtggtccacg
     4441 cgtccggcgg cggctacgac gaggttgcag gggccgtagc ggtcgcgcca gtacccggcg
     4501 acctggtcgc gtgcgacgcg gccgatcgtc tcgttgtagc cgaggaccgg ccggccgagc
     4561 ggatgcccgt cccacatcga ccggaagaag aggtcgtgca cgagctcgtc cggcgcgtcc
     4621 gcgtgcatcg cgatctcttc gaggatcacg ttgcgctcgg actcgagctc ggtcgggtcg
     4681 atcaccgagt tcaggatcat gtcgctcagc acgtccactg ccatcggcag gtcgtcgtcg
     4741 aggacgcgtg cgtagtagca cgtgtactcc ttgccggtga acgcgttgac gtcaccgccg
     4801 acggcgtcga acgactcggc gatctgccga gcggaacggg agcccggtcc cttgaccagc
     4861 aggtgctcga ggaagtgtga cgtgcccgcg agctcgtcgg gctcgtcacg ggcgccgacg
     4921 tcgaaccaga agccgaccga gatcgacctc acctcggtca tcgactcggt gacgacggtg
     4981 atccccgagt cgagctcggt ccgggcgaac ttcgtcacta cagggagtcc tcgtccgggc
     5041 ggcgcggggc acgctcgcgg cgcggccctc tgtcgccgcc gccaccgccg tcccacggcc
     5101 cccggtccag gccgccacgg acgcggccgc ctctgtcccg gtcgcggcca cctcggtccc
     5161 ggtcccggcc accacgatcg ccgccaccgc caccgccacc gcctgcgacg gctgcggcac
     5221 gggcttcctc gatcgtcttg ccggcctcga cctgacgctc gagccaggcg atcggcgtca
     5281 ggttgatgcg gccctgacgg tcgatgtcgg tgatctcgac ctcgatctgg tcgccgacgt
     5341 tcg
//
LOCUS       DASQDO010000408         8319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_471407, whole genome shotgun sequence.
ACCESSION   DASQDO010000408 DASQDO010000000
VERSION     DASQDO010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471407"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VEV43_11400"
     CDS             complement(<1..847)
                     /locus_tag="VEV43_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP24170.1"
                     /translation="MEKSKATEAAQLGRRDAFSSAAAHRKTEVKRIRERNRVKRQRYL
                     LIAIAAFAGFAAKRAFIDNDPLSFGLPNFSPDTMIYFIPPLMLLTLAMTPILVMMQGK
                     SPAIRFTPDQIGIGFDDVRGIDVVREEVTRTLQIFLAYRTFKEELGGNPRRGVLFEGN
                     PGTGKTHLAKAMAAEAGVPFFFVSAPAFQSMFFGMTAFRIRSFFKGLKKAARKEGGAI
                     GFIEEIDAVGMARAGMNAAPLPGPSRMGAFRFGSSDNGPMVNELLIQLQSFDTPPFFG
                     RIKNKG"
     gene            complement(902..1504)
                     /locus_tag="VEV43_11405"
     CDS             complement(902..1504)
                     /locus_tag="VEV43_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24171.1"
                     /translation="MLDRTLRLALRNYVTLFLFVATFTVAIHLVYLFLFRDVVGLAEI
                     HDAIREFPPQRQVRGVGHADLDAWRIGNVVVTLAEIALVPFLAKGAARVIEQDEKGEV
                     PTVGAALAATMKRGYRWFPSLREAGPLLVTVAIGLLTWWFVRKTGLLLVEPVGRDSEW
                     AAVAVVEGAARAAGAPFVLVSVVLRKSAPKADEGVPPSLY"
     gene            1529..2884
                     /locus_tag="VEV43_11410"
     CDS             1529..2884
                     /locus_tag="VEV43_11410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP24172.1"
                     /translation="MRIVLWHGYLLTGSGSNVFTANVARAWRAAGHDVVLMCQETLAG
                     VLEFVDSEADMSADNKTFVEYPTKAAPAPGRCRVVRPSIGEVLPVYVYDEYEGFTAKR
                     FVDLTEEELDAYVWRNVTALATVIRDHDPEAIVVGHEVMGPYVARLACEATGASYAVK
                     LHGSALEFAVKEQDRYRHYAHEGLTGAHAVVGGSNYMVAAASAVVPGWESRAQVVNPG
                     CDVDLFRPVEREPGHAPVAGYVGKLIPGKGVHHFLAALGATATEGLRAVVVGYGGFAR
                     ELKALAYTMATRNAGAAGKVAREASLDELDRFFETAPDAYWERAASVPVELAGRLEHG
                     PLSTTLPHWDVLVVPSEIPEAFGMVAAEAAASGVLPIVPRHSGIGEAGTAIERALDVP
                     GLLTFDPGDPVNGIAQRVDAVLSLPYDERRELELKAAELARREWSWDVVAARLLEAAT
                     A"
     gene            2881..3888
                     /locus_tag="VEV43_11415"
     CDS             2881..3888
                     /locus_tag="VEV43_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24173.1"
                     /translation="MTAFTATGTENLMVWDRRTAPFMEVWYATVTHRRLRSGLWLRYT
                     ISAPGSGDPSCALWACYFDPEGNRSFAGRNDHSIDLLGHVPGRDDGALVRIGDAWLSE
                     SHLEGRVESGGRSLAWSLDLEPAERCYHHLPAVLRHRAARRFSTLCSPNLSVPFSGTV
                     ELDGEVVAYEREPGCQTHRWGRRHSASWAWAHCSEWDDGGDAVFEAVAAKGAPWLPAL
                     TFPYLRYRGEDLVFTALRSRGRYEFPAWTFVASNDRWRISGAARLRLDRSVQMRYDDP
                     DGGTRHCVNSEIADLAIEVYERHERGWRLVDTLRSIGAAHFELGAPEVVGGVEVVTAG
                     T"
     gene            complement(3872..4693)
                     /locus_tag="VEV43_11420"
     CDS             complement(3872..4693)
                     /locus_tag="VEV43_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24174.1"
                     /translation="MRRVIHVIVGAVLLLGIVPGAALADHEEDGIYELPAQDDWTKTR
                     LTVYVVPPSHGQLYNSNGPLGGGDPNEVTPFENSYLRAMEDSIEEWNNGIRKFGSKAL
                     KRRFRADVYVLGRDPLPDEKEPDILVVTDENKGPVLGFALYGQPCIVNNSQMFVTSFS
                     YADMFNVMGQEYGHCLGLGHVGSQGGVEPTSSQKHPEHDVMNGFYADSIGAEGTHLHC
                     VSNLDVKGLEYTFVKTLTGSGEAQPVFMQVEKYRTTCGGDGRPSPEDQAAAKFLP"
     gene            complement(4757..5185)
                     /gene="arfB"
                     /locus_tag="VEV43_11425"
     CDS             complement(4757..5185)
                     /gene="arfB"
                     /locus_tag="VEV43_11425"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HYP24175.1"
                     /translation="MSGVRVNRSLEIPEDELRLEFTTSGGPGGQHANKASTRAVLIWN
                     VESSRVLGPRQRARIKGKLRHRIDSSGNLRLSSDAHRSQLRNREAVRERLGSLVEDAL
                     RPEKKRVGTKPTRSSQEKRLQSKRLRSDTKRTRAKPAPDD"
     gene            complement(5182..7179)
                     /locus_tag="VEV43_11430"
     CDS             complement(5182..7179)
                     /locus_tag="VEV43_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYP24176.1"
                     /translation="MSNRLAQETSPYLLQHKDNPVDWYPWGPDALERARSEDKPILLS
                     IGYAACHWCHVMERESFEDPAAAELMNEGFVCVKVDREERPDLDAIYMEAVQAMTGHG
                     GWPMTVFLTPDGAPFFGGTYFPPDDRHGLPGFKSLLRAVSDAWRDRRTEIDAQGQKLV
                     DHIGVATKLQPSHEVVTSDLVRGAGRTLAQSFDSEWGGFGQAPKFPQPMTIDFLLRLS
                     VHGDGTAAEMAEVTLDEMAAGGMYDQLGGGFARYSVDRQWIVPHFEKMLYDNAQLLRT
                     YARSWQLTRSERHRTVARETAEWMFREMRDPAGGFWSSLDADSEGEEGKFYVWSLDEV
                     REVAGEDADAAISYWGMTERGNFEGLNIPVLAGAEAPGPALGRARKALMERRATRVRP
                     GTDDKVLTAWNGLAAAALAEAGAALDEPRWVAIAGEVMDFVLGTMRVDGRLMRSYRQG
                     TVAHLGYSEDYAAVLEACLALWEATGELRWVTEARWAADDAVRLFLDPVSGGFYTTGS
                     DAETLVTRPKDLFDNAVPAANSILAVELQRLAALTGERTYESHALAIVRLVRNLVERS
                     PQGFGHLLGAIDLYTSGTVEVVVVGRDSDELVSRLRSTWRPNAVYAIVPEVTAESAAA
                     VPLLEGRREVEGRATAYVCRNGTCKLPVTTPDEMEEQLALQ"
     gene            7216..7503
                     /locus_tag="VEV43_11435"
     CDS             7216..7503
                     /locus_tag="VEV43_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24177.1"
                     /translation="MAAIAYVLLPVTGLLAYFSGRSPRVRWHGLQAIVLGALWPALLY
                     LAAAANESLVLPVAVAGAAVWLLFLAGAALGRDPSLPWLGPKLKELATDEL"
     gene            complement(7487..>8319)
                     /locus_tag="VEV43_11440"
     CDS             complement(7487..>8319)
                     /locus_tag="VEV43_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Ran-binding zinc finger domain-containing
                     protein"
                     /protein_id="HYP24178.1"
                     /translation="AASGSGDSGGRTGWPAPQEAAGASTSTVEDASSSPAAGSGARVL
                     YGDAGLTTATPSEAALRPAETEPADTTAAASAGAAGSGMAGTMGLDSGMGTSGGGGTD
                     AGTVGGLETGLGGSHMGTGETDAGTGFGTVETDAGTSAGDAGSGLGTTGVGAGTTDTT
                     AGTGGGGAVTERAASADDPQEQPFERDGRWWFRRGDELLVYEEQTGQWVAADEPSSSG
                     SVSTEASAPAETQAEPAPSEGQVTTQQEAVGTFWKCPSCGAVNGSTATSCRMCFTARP
                     "
BASE COUNT         1162 a         2991 c         2873 g         1293 t
ORIGIN      
        1 cgcccttgtt cttgatccgc ccgaagaacg gcggcgtgtc gaaggactgg agctggatca
       61 ggagctcgtt gaccatcggg ccgttgtcgc tcgaaccgaa gcggaacgcg cccatccgcg
      121 acgggcccgg cagcggcgcc gcgttcatcc cggcgcgtgc catgccgacc gcgtcgatct
      181 cctcgatgaa gccgatcgcg ccgccctcct tgcgggccgc cttcttcagg cccttgaaga
      241 acgaacggat acggaacgcc gtcatgccga agaacatcga ctggaacgcg ggggccgaga
      301 cgaagaagaa cgggacgccg gcctccgcgg ccatcgcctt cgcgaggtgc gtcttgccgg
      361 tcccggggtt gccctcgaac aggacgccgc ggcgggggtt cccgccgagc tcctccttga
      421 acgtccggta cgcgaggaag atctggagcg tgcgcgtcac ttcctcgcgc acgacgtcga
      481 tgccgcggac gtcgtcgaac ccgatgccga tctggtccgg ggtgaacctg atcgccggag
      541 acttgccctg catcatcacg aggatcggcg tcatcgcgag cgtcaggagc atcagcggcg
      601 ggatgaaata gatcatcgtg tccggcgaaa agttgggcag gccgaacgag agcgggtcgt
      661 tgtcgatgaa cgcgcgcttc gccgcgaagc ccgcgaacgc cgcgatcgcg atcagcagat
      721 agcgctgccg cttgacgcgg ttgcgctcgc ggatcctctt gacctcggtc ttgcggtgcg
      781 cggccgccga gctgaacgcg tctcggcgcc ctagctgcgc ggcctctgtc gccttcgatt
      841 tctccactga cgtggtcctt ccgcgatcgc actgcgtctg ccgtattgga agattctggc
      901 atcagtagag actcggcgga accccctcat cggctttagg agccgattta cgtagaacta
      961 cggagacgag cacgaagggg gccccggccg cccgggcggc cccctcgacg accgcgacgg
     1021 ccgcccactc ggaatctcgc cccaccggct cgacgaggag caggccggtc ttccgcacga
     1081 accaccaggt caggaggccg atcgcgaccg tcacgagcag cggaccggcc tcccggaggg
     1141 agggaaacca ccggtacccg cgcttcatcg tcgccgccag ggccgcgccg accgtcggaa
     1201 cctcgccctt ttcgtcctgc tcgatgaccc gggcggcccc cttggcgagg aacggcacga
     1261 gcgcgatctc cgccagcgtc acgacgacgt tgccgatccg ccaggcgtcc aggtcggcgt
     1321 ggccgacgcc gcggacctgg cgctgcgggg ggaactcccg gatcgcgtcg tggatctcgg
     1381 ccagcccgac gacgtcccgg aaaaggaaga ggtagacgag gtggatcgcg acggtgaacg
     1441 tcgcgacgaa gaggaagagc gtgacgtagt tgcggagggc gagcctgagc gtccggtcca
     1501 gcacggtcgg agtctaggct ccgcgcccat gcggatcgtg ttgtggcacg gctacctgtt
     1561 gaccggctcg ggctcgaacg tcttcaccgc gaacgtcgcc cgggcctggc gcgcggccgg
     1621 gcacgacgtc gtgctgatgt gtcaggagac gctcgccggc gtgctcgagt tcgtcgactc
     1681 ggaagccgac atgtccgccg acaacaagac gttcgtcgag tatccgacga aagccgcgcc
     1741 ggcccccggg cggtgccgcg tcgtccggcc ctccatcggc gaggtcctcc ccgtctacgt
     1801 ctacgacgag tacgagggct tcaccgccaa gcggttcgtc gacctgacgg aagaagagct
     1861 cgacgcttac gtgtggcgca acgtgacggc gctcgcgacc gtgatccgtg accacgaccc
     1921 cgaggcgatc gtcgtcggcc acgaggtcat gggcccgtac gtcgcgcggc tcgcttgcga
     1981 ggcgacaggc gcgtcgtacg ccgtgaaact gcacgggagc gcgctcgagt tcgcggtgaa
     2041 ggagcaggac cgctaccgcc actacgccca cgagggcctg accggcgcgc acgcagtggt
     2101 cggtggcagc aactacatgg tcgcggccgc gtcggccgtg gtccccggat gggagtcgcg
     2161 cgcgcaggtc gtgaaccccg gctgcgacgt cgacctcttc cgcccggtcg agagggagcc
     2221 cgggcacgcg ccggtcgcgg gctacgtcgg caagctgatc cccggcaagg gcgtgcacca
     2281 cttcctcgct gcgctgggcg cgacggcaac ggaaggtctg cgcgcggtcg tcgtcggcta
     2341 cggcgggttc gcacgcgagc tcaaggcgct cgcctacacg atggcgacgc gcaacgcggg
     2401 ggccgcgggc aaagtcgctc gggaggcgtc gctggacgag ctcgatcggt tcttcgagac
     2461 ggcgccggac gcgtactggg agcgggccgc gtcggtcccg gtcgagctcg ccggccgtct
     2521 cgagcacggc cccctctcca cgacgcttcc gcactgggac gtgctggtcg tcccctccga
     2581 gatacccgag gccttcggca tggtcgcggc cgaagccgcg gcgagcggcg tccttccgat
     2641 cgtcccgcgc cattcaggca tcggcgaggc ggggaccgct atcgagcgcg cgctggacgt
     2701 gccgggtctg ctcacgttcg accccggcga ccccgtgaac gggatcgcgc aacgggtgga
     2761 cgccgtgctg tcgctcccgt acgacgaacg acgcgagctc gagctgaagg cggcagagct
     2821 agcgcggcgc gagtggtcgt gggacgtggt cgccgcgcgt ctgctggagg cggcgacggc
     2881 gtgacggcct tcaccgcgac cgggaccgag aacctcatgg tgtgggaccg gaggacggcg
     2941 ccgttcatgg aggtctggta cgcgaccgtc acgcatcgcc gcctgcgcag cggcctgtgg
     3001 ctgcgctaca cgatctcggc ccccggctcc ggcgatccct cgtgcgcgtt gtgggcgtgc
     3061 tacttcgatc ccgagggcaa ccggtcgttc gccggcagga acgaccactc gatcgacctc
     3121 ctcggccacg tccccggccg ggacgacggc gccctcgtca ggatcggcga cgcgtggctg
     3181 tcggagagcc atctggaggg ccgcgtcgag tccggcggga ggagcctcgc gtggtcgctc
     3241 gacctcgagc ccgcggagcg ctgctaccac cacctgcccg cggtgctgcg gcaccgagcg
     3301 gcgcggaggt tctcgacgct gtgctcgccg aacctgtccg tccccttctc cgggacggtc
     3361 gagctcgacg gcgaggtcgt ggcgtacgag cgggagccgg gctgccagac gcaccggtgg
     3421 ggacgcagac actcggcgtc gtgggcgtgg gcgcactgct cggagtggga cgacggcgga
     3481 gacgcggtct tcgaagccgt cgcggccaag ggggccccgt ggcttcccgc gctgacgttt
     3541 ccctatctgc gctaccgggg tgaagacctc gtcttcacgg cgctgcggtc gcgcggccgc
     3601 tacgagttcc ccgcgtggac cttcgtcgcg tcgaacgacc ggtggcggat ctcaggcgcg
     3661 gcccggctcc gtctcgaccg ttcggtccag atgcgctacg acgatcccga cggtgggacg
     3721 cggcactgcg tgaactccga gatcgccgac ctcgcgatcg aggtctacga acgacacgag
     3781 cggggctggc gcctcgtcga cacgctccgg tcgatcggcg ccgcgcactt cgagctcggc
     3841 gcgccggagg tagtcggcgg cgtcgaggtc gttacggcag gaacttagcc gcggcctggt
     3901 cctcgggcga cggacgtccg tcgcccccgc aggtcgtccg gtacttctcg acctgcatga
     3961 agaccggctg cgcctcgccg ctccccgtca gcgtcttcac gaacgtgtac tcgaggcctt
     4021 tgacgtcgag gttgctgacg cagtggaggt gcgtcccctc ggcaccgatc gagtccgcgt
     4081 agaagccgtt catgacgtcg tgctcggggt gcttctgcga cgaggtcggc tcgacgccgc
     4141 cctggctccc gacgtgaccg aggcccaggc agtggccgta ctcctggccc atgacgttga
     4201 acatgtccgc gtagctgaag ctggtcacga acatctgcga gttgttgacg atgcagggct
     4261 ggccgtagag ggcgaagccg aggaccgggc ccttgttctc gtcggtgacg acgaggatgt
     4321 cgggctcctt ctcgtccggc agggggtcgc gcccgaggac gtacacgtcc gcacggaagc
     4381 gcctcttcag ggccttcgag ccgaacttcc ggatcccgtt gttccactcc tcgatcgagt
     4441 cctccatcgc gcggaggtag ctgttctcga acggcgtgac ctcgttgggg tcgcctccgc
     4501 cgagggggcc gttcgagttg tagagctgcc cgtggctggg gggcacgacg tagaccgtga
     4561 ggcgcgtctt cgtccagtcg tcctgcgccg gcagctcgta gatgccgtcc tcttcgtggt
     4621 cggccagggc ggcccccggc acgatcccga gcaacagcac cgcaccgacg atgacgtgga
     4681 tgaccctgcg cattctttcc tcctgtgagt ccgctcctcc ccgggttctc cggttcgcgc
     4741 agaagtcctc tgacggtcag tcgtcaggcg ccggcttggc ccgcgtccgc ttcgtgtccg
     4801 agcgcaggcg tttcgactgc aagcgcttct cctgggacga ccgggtcggc ttggtgccga
     4861 cccgcttctt ctcgggccgg agcgcgtcct ctaccagcga gccgagccgc tccctgactg
     4921 cttcccggtt gcgcagctgg gaccggtggg cgtcgctcga gagccggagg ttccccgacg
     4981 agtcgatccg gtggcggagc ttccccttga tccgggccct ctgacggggg ccgaggaccc
     5041 gcgacgactc gacgttccag atcaggaccg cacgggtcga cgccttgttc gcgtgctggc
     5101 cgcccggccc ccccgacgtc gtgaactcga ggcggagctc gtcctccggg atctccagcg
     5161 aacggttcac gcgcacgccg ctcattgcag cgccagctgc tcctccatct cgtccggcgt
     5221 cgtcaccggc agcttgcacg tgccgttcct gcacacgtag gccgtcgccc ggccctcgac
     5281 ctcgcgccgg ccttcgagca gcgggacggc cgccgcgctc tcggccgtga cctcgggcac
     5341 gatcgcgtac accgcgttcg gacgccacgt cgagcgcagc cgggacacga gctcgtccga
     5401 gtcccggccc acgacgacga cctcgacggt cccggaggtg tagaggtcga tcgcaccgag
     5461 gaggtggccg aaaccttgtg gcgaccgctc gacgaggttg cggaccagcc ggacgatcgc
     5521 gagcgcgtgg ctctcgtacg tccgctctcc ggtgagcgcg gcgagtcgct ggagctcgac
     5581 ggcgaggatc gagttcgccg ccgggaccgc gttgtcgaac aggtccttcg ggcgcgtgac
     5641 gagcgtctcg gcgtcggacc ccgtcgtgta gaacccaccg gagaccgggt cgaggaacag
     5701 acgaacggcg tcgtcggccg cccagcgcgc ctcggtcacc cagcggagct cgccggtcgc
     5761 ctcccacagc gcgaggcacg cctcgagcac cgcggcgtag tcctcggagt agccgaggtg
     5821 cgcgaccgtc ccctgccggt aggaccgcat cagccggccg tcgacgcgca tcgtgccgag
     5881 cacgaagtcc atgacctcgc ccgcgatcgc gacccagcgc ggttcgtcga gcgcggcccc
     5941 cgcttccgcg agcgccgcag ccgcgaggcc gttccacgcc gtcagcacct tgtcgtcggt
     6001 gccgggacgg acacgcgtcg cgcgccgttc catcagagcc ttcctggccc gcccgagcgc
     6061 ggggcccggc gcttccgccc ccgccaggac ggggatgttc aggccctcga agttcccgcg
     6121 ctcggtcatc ccccagtacg agatcgccgc gtcggcgtcc tcgccggcca cctcgcggac
     6181 ctcgtccagc gaccaaacgt agaacttgcc ctcttcgccc tcggagtccg cgtccagcga
     6241 cgaccagaac ccgcccgcgg ggtcgcgcat ctcgcggaac atccactcgg cggtctcgcg
     6301 tgcgacggtg cggtgccgtt ccgaacgcgt gagctgccac gaacgcgcgt aggtcctcag
     6361 cagctgcgcg ttgtcgtaga gcatcttctc gaagtgcggg acgatccact gacggtcgac
     6421 cgaatagcgg gcgaaccctc cgccgagctg gtcgtacatc ccgcccgcgg ccatctcgtc
     6481 gagcgtcacc tccgccatct ccgcagcggt gccgtcgcca tggacagaga gacgcagaag
     6541 gaagtcgatc gtcatcggct gcgggaactt gggcgcctgc ccgaagccgc cccactcgga
     6601 gtcgaacgac tgcgcgagcg tccgcccggc ccccctcacg agatccgacg taacgacctc
     6661 gtgcgacggc tgcagcttcg tcgcgacgcc gatgtggtcg acgagcttct gtccctgcgc
     6721 gtcgatctcg gtgcggcggt cgcgccacgc gtcggagacc gcacgcagca ggctcttgaa
     6781 acccggcagc ccgtggcggt cgtcgggcgg gaagtaggtc ccgccgaaga acggagcgcc
     6841 gtccggcgtg aggaacacgg tcatcggcca cccgccgtgg ccggtcatgg cctggacggc
     6901 ctccatgtag atcgcgtcga ggtcgggccg ctcctcgcgg tcgaccttca cgcagacgaa
     6961 gccttcgttc atcagctcgg cggccgccgg gtcctcgaac gactcgcgct ccatcacgtg
     7021 acaccagtgg caggcggcgt aaccgatcga cagcagtatc ggcttgtcct cggagcgcgc
     7081 ccgctccagc gcgtccggcc cccacggata ccagtcgacg gggttgtcct tgtgctgcag
     7141 gaggtaaggg ctcgtctctt gtgccaggcg gttgctcatg tcctcagttc tccccgactt
     7201 aatgtgggcc acaccatggc tgcgatcgct tacgtgctgc tccccgtgac cgggctgctc
     7261 gcgtacttct cgggccgcag ccctcgcgtc cggtggcacg gcctgcaggc gatcgtcctc
     7321 ggcgcgctgt ggccggcgct tctctacctc gccgcagcgg cgaacgagtc gctcgtgctc
     7381 cccgtcgcgg tcgcgggggc cgccgtgtgg ctgctgttcc tcgcgggggc cgcgctcggc
     7441 agggacccga gcctgccgtg gctcgggccg aagctgaagg agctcgctac ggacgagctg
     7501 tgaaacacat gcggcacgac gtcgcggtcg agccgttgac tgcgccgcac gaagggcact
     7561 tccagaacgt gccgacggcc tcctgctgcg tggtgacctg gccctcggag ggagcgggct
     7621 ccgcctgagt ctccgcgggg gccgaagcct ccgtggagac gctcccggag cttgacggct
     7681 cgtccgccgc cacccactgc ccggtctgct cctcgtagac gagcagctcg tcgccccgcc
     7741 ggaaccacca gcggccgtcg cgctcgaagg gctgctcctg aggatcgtcg gccgaggccg
     7801 cccgttcggt gactgcgccg cccccgccgg tgcccgcggt ggtgtcggtc gtcccggccc
     7861 ccaccccggt cgtgccgagg cccgacccgg cgtccccggc cgaggtcccc gcatccgtct
     7921 cgacggtgcc gaagccggtc ccggcgtccg tctcgcccgt acccatgtgg gacccgccga
     7981 gcccggtctc gaggccgccg accgtccccg cgtccgtccc gccgcccccc gacgttccca
     8041 tgccggagtc gagccccatc gtgccggcca tcccggaccc ggccgcaccc gccgaagcgg
     8101 ctgcggtcgt gtccgcgggc tcggtctcgg ccgggcgcag cgcagcctcg ctcggcgtcg
     8161 cggtcgtcag cccggcgtcc ccgtacagca cccgcgcacc gctaccggcc gcaggcgacg
     8221 aagaggcgtc ctctacggtc gaggtcgagg ccccggccgc ttcctgcggc gcaggccagc
     8281 ccgtccggcc cccggagtca ccggaccccg aggccgcag
//
LOCUS       DASQDO010000409         4614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1205749, whole genome shotgun sequence.
ACCESSION   DASQDO010000409 DASQDO010000000
VERSION     DASQDO010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205749"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VEV43_11445"
     CDS             complement(<1..943)
                     /locus_tag="VEV43_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYP24179.1"
                     /translation="MTTTSPFGARKALATGSGELDYFGLTALADAGVADLDKLPVTVK
                     ILLENLLRLATTRHADEGDVELLAGWGQGSVEEREFPFAPARVILQDFTGVPATVDLA
                     AMRTAIEREGGDPSKVDPLVPVDLVIDHSVQVDSFGSAQSYTINVEREYERNHERYSL
                     LRWAQQAFAGFRVVPPGMGIVHQVNLEHIARVVQDVDGVAVFDTLVGTDSHTTMINGL
                     GVLGWGVGGIEAEAALLGQPLPLVTPKVVGFRLTGHLQTGVTATDLVLTVTEMLRKHG
                     VVGKLVEFYGEGLSKLSLPDRATIANMAPEYGATCSFF"
     gene            1000..2517
                     /locus_tag="VEV43_11450"
     CDS             1000..2517
                     /locus_tag="VEV43_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HYP24180.1"
                     /translation="MAFDGDGWRIDGFDESPPDDLAARIWKRDASVWGPGDDDPADRL
                     GWLDLPDTIRGEIAPVEAFVRDTGVYMDKVVLLGMGGSSLAPEVFATCFGARTGYPQL
                     VVLDSTHPGQIEPVAEELVGTNNLFVVSSKSGGTIETTSLYAYFRARSDDARNFAAIT
                     DPGTSLEQLASDEGFGGVFLNPPDVGGRYSALSLFGLVPAALCGVDLDALLEPAAAMA
                     AACGPSVPPDENPGLVLGWALGRLAQGGRDKLTFVTSPGLAPFGDWVEQLIAESTGKH
                     GVGIVPVVGEPPLDTYGEDRVFVHLRLEDDELPKPPSGHPVIRLDVRGPEELGAQMFL
                     WEFATAVASAALGINAFDQPDVEAAKKAARAALESDEEPDWTEDDVDALFAGAAPGDY
                     AAFLAFAPRTVETTETLDAARAKVARSQGIATTAGVGPRYLHSTGQLHKGGPDSVRAV
                     VILDAPESETGIPGQPYDFGHLLRAQATGDYEALKSAGKKVARTTWSRFEEWART"
     gene            2484..3425
                     /locus_tag="VEV43_11455"
     CDS             2484..3425
                     /locus_tag="VEV43_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24181.1"
                     /translation="MEPFRGVGENVTKGLTGFGEFAGAFPDSDLAVGWGRFDAVHVFS
                     LSRRELLGSFASIFERGGRRLAVVDGDPPVVVAGAWDRYGVAGYGIDGELVWERRDLK
                     KSQQLHPVGKGGVAVGMEYKMMHVVEATTGETVRTVRGADYLCHDGRSSLVMAVRRGS
                     YDLVAYAVPEWRQAWKVQLSGVIDVALSQDAVLTTGTEGHAHLNPSRSLTFDPKGVTC
                     VDMSGAVAWTWTPKTSGHVFRVAWSRALGAWAGLYRAYERLPDSLLVWSTTGELLHSV
                     QLSHRPLAFASEGSLLLTNGGEVLDVPSGRVLWKLTF"
     gene            complement(3430..4095)
                     /gene="hemQ"
                     /locus_tag="VEV43_11460"
     CDS             complement(3430..4095)
                     /gene="hemQ"
                     /locus_tag="VEV43_11460"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HYP24182.1"
                     /translation="MSEDLVYASYPVFRANARAAGLDRDLAAKQLEELFAEWEDRVQV
                     RGAYSTAAFTADADLMFWWVAKSVDDLQQLTIEFARTHLGSLCAPVHAFLGVVQPAEF
                     ARDHQPAFVKGEPPKKYACVYPFVRTPEWYLLDPAERGKLLREHGEGGREFPEVLANT
                     TSAFGLGDYEWILAFEADSPDRIVQLIRRLRATEARRYTKLETPFITGIRKNLGGLVA
                     DLV"
     gene            4162..>4614
                     /locus_tag="VEV43_11465"
     CDS             4162..>4614
                     /locus_tag="VEV43_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HYP24183.1"
                     /translation="MPQPSIVTTTDYHTGGEPFRIVTGGVPPLEGRSILDKRRYAKEH
                     LDDVRKLLVYEPRGHADMYGCFVTEPEDADGHLGVVFFHNEGYSTACGHGTIALVTWA
                     LESGRVPPTSDHVFVDAPSGRLTTVARMDGDRVAAVRFTNVPSFVWDTG"
BASE COUNT          712 a         1556 c         1612 g          734 t
ORIGIN      
        1 ggaagaagct acaggtcgcc ccgtactcgg gggccatgtt cgctatcgtc gcccggtcgg
       61 ggagggagag cttgctcaag ccctcgccgt agaactcgac gagcttgccg acgacgccgt
      121 gcttgcggag catctcggtc acggtgagca cgaggtcggt cgcggtgacg cccgtctgga
      181 gatggcccgt gaggcggaag ccgactacct tcggcgtcac gagcggaaga ggctggccga
      241 gcagcgcggc ctcggcttcg atcccgccga cgccccagcc gagcacgccg agcccgttga
      301 tcatcgtcgt gtgcgagtcg gtgccaacca gggtgtcgaa cacggcgact ccgtcgacgt
      361 cctgcacgac gcgggcgatg tgctcgaggt tcacctggtg gacgatcccc atgccgggcg
      421 ggacgacgcg gaagccggcg aacgcctgct gcgcccaacg gagcagcgag tagcgctcgt
      481 ggttacgctc gtactcgcgc tcgacgttga tcgtgtacga ctgcgcgctc ccgaacgagt
      541 cgacctgcac tgaatggtcg atcacgaggt cgaccggcac gagcgggtcg accttggacg
      601 ggtcgccgcc ctcccgctcg atcgcggtgc gcatcgcggc gaggtcgacg gtcgcaggta
      661 cgccggtgaa gtcctgcagg atcacgcggg ccggagcgaa cggaaactcg cgctcctcca
      721 ccgagccctg cccccatccc gcgaggagct cgacgtcgcc ctcgtcggcg tgacgcgtcg
      781 tagcgagccg gagcaggttc tcgagcagga tcttgacggt cacggggagc ttgtcgaggt
      841 cggcgacgcc cgcgtccgcg agcgccgtga gtccgaagta gtcgagctcg ccggagcccg
      901 tggcgagcgc cttgcgcgcg ccgaaaggtg aggtggtcgt catcggcttc ctccttgtgt
      961 tctgctccca cgctaacgcc cgcggatagg gtgcgtggca tggctttcga cggcgacggc
     1021 tggcggatcg acgggttcga cgagagtccg ccggacgatc tcgcggcgcg catctggaag
     1081 cgcgacgcga gcgtgtgggg gccgggtgac gacgacccgg cggaccggct cgggtggctc
     1141 gacctgccgg acacgatccg gggtgagatc gctccggtcg aggcgttcgt gcgcgacacc
     1201 ggcgtctaca tggacaaggt cgtgctcctg ggcatgggtg gatcgagcct cgcgcccgag
     1261 gtgttcgcga cgtgcttcgg cgcgcgcacc ggctacccgc agctcgtggt cctggactcg
     1321 acgcaccctg ggcagatcga gccggtcgcg gaggagctcg tcgggacgaa caacctcttc
     1381 gtcgtgtcga gcaagtccgg cgggacgatc gagacgacgt ctctgtacgc gtacttccgc
     1441 gcgcgatcgg acgacgcacg caacttcgcc gcgatcaccg atcccggcac gtcactcgag
     1501 caactcgcgt ccgacgaggg cttcggcggc gtcttcttga acccgccgga cgtcggcgga
     1561 cggtattccg cgctgtcgct gttcgggctc gtgcccgcgg ctctgtgcgg agtcgacctc
     1621 gacgctctgc tcgagcccgc agcggccatg gccgctgcgt gcgggccatc ggtcccgccg
     1681 gacgagaacc ccgggctggt cctcgggtgg gcgctgggcc gcctcgcgca ggggggccgc
     1741 gacaagctca cgttcgtgac gtcgccgggg ctcgcgccgt tcggcgactg ggtcgagcag
     1801 ctgatcgccg agtcgacggg caagcacggg gtggggatcg tgccggtggt cggtgagccg
     1861 cctctcgaca cgtacgggga ggacagggtc ttcgtccacc tgcgactgga ggacgacgag
     1921 ctgccgaaac cgccgtccgg gcacccggtg atccggctgg acgtgcgcgg gcccgaggag
     1981 ctgggggccc agatgttcct gtgggagttc gcgacggcgg tagcgagcgc ggccctaggg
     2041 atcaacgcgt tcgatcagcc cgacgtcgag gcggccaaga aagcggcccg tgcggccctt
     2101 gagtcggacg aggagccgga ctggacggaa gacgacgtcg acgcgctgtt cgccggcgcg
     2161 gcccccggtg actacgcggc gttcctggct ttcgcgccgc gcacggtcga gacgacggag
     2221 accctggacg cggcccgagc caaggtcgcg cgctcgcaag ggatagcgac gacggccggc
     2281 gtaggccccc gctatctgca ctcgaccggt cagttgcaca aagggggccc ggactccgtg
     2341 agggccgtgg tgatcctgga cgcaccggag tcggagaccg gcatccccgg ccagccatac
     2401 gacttcgggc acctcctgag agcgcaagca acgggcgact acgaagccct gaaatcggcg
     2461 ggcaagaagg tagcccgcac gacgtggagc cgtttcgagg agtgggcgag aacgtaacga
     2521 agggcctcac gggattcggg gagttcgccg gcgcgttccc cgactccgac ttagccgtcg
     2581 gatggggacg gttcgatgcc gtccacgtct tcagcctctc ccggcgcgag cttctcggct
     2641 cgttcgcgtc gatcttcgag cgcggggggc gccgtctggc cgtcgtcgac ggcgacccac
     2701 ccgtggtcgt cgccggagcg tgggatcggt acggcgtcgc gggatatggg atcgacggcg
     2761 aactcgtctg ggagaggcgc gacctgaaga aatcacagca acttcacccc gtgggcaagg
     2821 ggggcgtcgc cgtcggcatg gaatacaaga tgatgcacgt cgtcgaagcc actacgggcg
     2881 agacggtacg gaccgttcga ggcgcggact acctctgcca cgacggccgc tcctcgctcg
     2941 tgatggccgt tcgccggggt agttacgacc tggtggcgta cgcggtgccc gagtggcgac
     3001 aagcgtggaa ggttcaacta tcgggcgtga tcgacgtcgc tctttcgcag gacgcggtac
     3061 tgacgacggg aaccgagggt cacgcgcacc tcaacccttc gcgcagtctc acgttcgacc
     3121 cgaaaggcgt cacctgcgtg gacatgtcgg gtgcagtcgc ttggacctgg accccgaaaa
     3181 ccagtgggca cgtgttccga gtcgcgtgga gccgggccct cggtgcatgg gccggcctct
     3241 atcgcgcgta cgaacgcctg ccggactccc tgctcgtatg gtcgactacg ggagagctcc
     3301 tccactcggt gcaactgagc cacagaccgc tggcgttcgc ctccgagggg tccctactgt
     3361 tgaccaatgg gggcgaggtc ctcgacgttc cgtccgggcg cgtgctctgg aagctcacct
     3421 tctgatcgat cagaccaggt cggccaccag gccccccaaa ttctttcgga tccccgttat
     3481 gaacggcgtc tccagcttcg tgtagcgtct cgcctccgtc gccctcagtc tccttatcag
     3541 ttgcactatc cggtccggcg agtcggcctc gaaggccagg atccattcgt agtcgcccag
     3601 gccgaacgcg ctcgtcgtgt tcgccagcac ctccgggaac tcgcggcccc cttcgccgtg
     3661 ctcgcgcagc agcttgcccc gctccgccgg gtccagcagg taccactccg gcgtccgcac
     3721 gaacgggtac acgcacgcgt acttcttcgg cggctcgccc ttgacgaacg cgggctggtg
     3781 gtcgcgcgcg aactccgccg gctgcacgac gcccaggaac gcatggaccg gcgcgcacaa
     3841 cgagcccagg tgcgtcctgg cgaactcgat cgtgagctgc tggaggtcgt cgacggactt
     3901 cgcgacccac cagaacatca ggtcggcgtc ggcggtgaac gcggcggtcg agtacgcgcc
     3961 gcggacctgg acacggtcct cccactcggc gaacagctcc tcgagctgct tcgccgcgag
     4021 gtcgcggtcc agccccgccg ctctcgcatt cgcacggaac acggggtagc tcgcgtacac
     4081 gaggtcttcg gacatcgggc ggtgcctttc tctcgaaaac ggtcgtgctc cgcacactac
     4141 cggggctgcg aagatggccc catgccacag ccctccatcg tcacgacgac cgactaccac
     4201 acgggcggag agccgttccg catcgtgacc ggcggcgttc ccccgctcga aggccgctcg
     4261 atcctcgaca agcgccgcta tgcgaaggag cacctcgacg acgtccgcaa gctcctcgtc
     4321 tacgagcccc gtggccacgc ggacatgtac gggtgcttcg tgaccgagcc cgaggacgcc
     4381 gacggtcacc tcggcgtcgt cttcttccac aacgagggct actcgaccgc gtgcgggcac
     4441 gggacgatcg cgctcgtgac gtgggcactc gagtcgggac gcgtcccgcc gacgtcggat
     4501 cacgtcttcg tcgacgcgcc ctcgggacgg ctgacgaccg tcgcgcggat ggacggcgat
     4561 cgcgtagcgg ccgtccgctt cacgaacgtc ccgtcgttcg tgtgggacac gggc
//
LOCUS       DASQDO010000410         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_594184, whole genome shotgun sequence.
ACCESSION   DASQDO010000410 DASQDO010000000
VERSION     DASQDO010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594184"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VEV43_11470"
     CDS             <1..1017
                     /locus_tag="VEV43_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYP24184.1"
                     /translation="DDERTAGVVAASAGNHAQGVALAASLAGIEATVYMPAGASLPKA
                     EATKRYGATVVLEGHDFGAAFKAAEEHAARTGATFVHPFDHEHVIAGQGTIGLEISEQ
                     MNGDIGTVVIPIGGGGLCAGIAAAVKALHPAARVLGVQASGAASFQRSLAQGSPVTLD
                     SMSTIADGIAANRPGDLTLAHVAEHVDEVVCVSDDAIAEALVMTAERMKLVLEPAGAA
                     GVAAVTQGVGELEPPVVVILSGGNIDPLLLLRVMRHGLSATGRYLAFRTRVPDKPGEL
                     FKLLGLIAEMGANVVGVEHHREGVAVQMDEVEVTLQIETKGGPHIQQVTGRLASAGYS
                     IERL"
     gene            1060..>1519
                     /locus_tag="VEV43_11475"
     CDS             1060..>1519
                     /locus_tag="VEV43_11475"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HYP24185.1"
                     /translation="MNNELDLSLVRPLVESWLAEDVGRGDVTSASVIPEKAYAVARLE
                     ARESFVVAGIDVAGLCFEIVGGGRLDWDPKVDDGHVTQAAETLARVEGNLRSILTAER
                     TAINAFARLSGVASLTSDFAQSLWGTKARLVDTRKTTPGLRVLEKYAVRVG"
BASE COUNT          219 a          524 c          562 g          214 t
ORIGIN      
        1 gacgacgagc ggaccgcggg cgtcgtggcc gcgtcggccg gcaaccacgc ccagggcgtc
       61 gcgctggccg cttcactcgc cgggatcgag gcgaccgtct acatgccggc gggagcgtcc
      121 ctgccgaagg ccgaggcgac gaagcgctac ggcgcgaccg tcgtgctcga gggccacgac
      181 ttcggggccg cgttcaaggc cgcggaggag cacgcggctc gcaccggcgc gacgttcgtc
      241 catccgttcg accacgagca cgtgatcgcc gggcagggga cgatcggcct cgagatctcc
      301 gagcagatga acggcgacat cggcaccgtc gtcatcccga tcggcggcgg cgggctctgc
      361 gccgggatcg cggcggcggt caaggccctc catcccgcgg cgcgggtgct cggcgtccag
      421 gcttcggggg cggcgtcgtt ccagcgctcg ctcgcgcagg gctcaccggt gacgctcgac
      481 tcgatgtcga cgatcgcgga cgggatcgcc gcgaacaggc cgggtgacct cacgctcgcg
      541 cacgtcgccg agcacgtcga cgaggtcgtg tgcgtctcgg acgatgcgat cgcggaagct
      601 ctggtgatga cggccgagcg catgaagctc gtgctcgagc ccgcgggggc cgcgggcgtc
      661 gccgcggtga cgcaaggcgt gggcgagctc gagcccccgg tcgtcgtgat cctctcgggc
      721 gggaacatcg acccgctgct gctgctgcgc gtgatgcgtc acggcctcag cgcgaccggc
      781 cgctacctcg cgttccgcac gagggtcccg gacaagccgg gtgagctctt caagctgctc
      841 ggcctcatcg cggagatggg cgcgaacgtc gtcggcgtcg agcatcaccg cgagggcgtc
      901 gccgtccaga tggacgaggt cgaggtgacg ctccagatcg agacgaaggg cggcccccac
      961 atccagcagg tcaccgggcg cctggcctcc gccgggtatt cgatcgagcg actctgatcc
     1021 cccttccggc aggggggccg gggccataac ctgcgtacgg tgaacaacga gctcgatctg
     1081 tctctcgtcc ggcccctggt cgagtcctgg ctcgccgagg acgtaggccg cggcgacgtc
     1141 acgtcggcga gcgtcatccc cgagaaggcc tacgcggtcg cacgcctgga ggcccgggag
     1201 agcttcgtcg tcgccgggat cgacgtcgcg gggctctgct tcgagatcgt cggcgggggg
     1261 cggctcgact gggacccgaa ggtcgacgac ggtcacgtga cgcaagcggc cgagacgctc
     1321 gcccgggtcg aggggaacct ccggtcgatc ctcacggccg agcggaccgc gatcaacgcc
     1381 ttcgcccggc tctcgggggt tgcgtccctc acctccgact tcgcccagag cctctggggg
     1441 acgaaggcgc gcctcgtcga caccaggaag acgacgcccg gtctgcgcgt tctagagaag
     1501 tacgcagtga gggttggcg
//
LOCUS       DASQDO010000411         6875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_961367, whole genome shotgun sequence.
ACCESSION   DASQDO010000411 DASQDO010000000
VERSION     DASQDO010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961367"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VEV43_11480"
     CDS             <1..477
                     /locus_tag="VEV43_11480"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HYP24186.1"
                     /translation="AKVSALGRAPLFIPEQKKVAELLREMQEQHVHMAIVVDEYGGTA
                     GLVTIEDLIEEIVGEIVDEYDQEEPLVEPVDEDRIRVDAKMPIDEVNELLEVDLPHDE
                     WDTVGGLVFGLTGRVPQVGESVRFDSLEFRTERVTGRRVQKVLITKTPAPEQGDDD"
     gene            473..1366
                     /gene="era"
                     /locus_tag="VEV43_11485"
     CDS             473..1366
                     /gene="era"
                     /locus_tag="VEV43_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HYP24187.1"
                     /translation="MSEPFRSGFVAVVGRPNVGKSTLVNALVGTKVAITSDKPQTTRS
                     AIRGILDRDGVQVVLVDTPGYHKPRTLLGQRLNDLVRATWKDVDLALFVVDGKSGVGR
                     GDEKVAADLAAAQCPTFCIVNKVDAMGRDDVAVALTQAAAIRDFDEFVPVSARTGEGV
                     ELLEGLVTARMPEGPMYYPPGTRTDQPPPAFVAELVREKLLARTREELPHSIAVVTED
                     FEERDDGLLEIRATVFVERESQKGIVIGKGGETVKAVGTETRLEIEALFGTKVFLELR
                     AKVEKDWQRRAHALERLGFGG"
     gene            complement(1363..1956)
                     /locus_tag="VEV43_11490"
     CDS             complement(1363..1956)
                     /locus_tag="VEV43_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24188.1"
                     /translation="MKRVALLLAISGLMPLVVAPASDAGTFTADVYRSHFLARDRDGV
                     AYSVDAVVEDRPGGTQLVLEVRRRCKGCRAEVYVRELGPGDFALVPPVGVTECVCMGA
                     FVHTKFGGKELRIEWRWDLEQDGGPAGDGYEWATVTANNLMNVSCFGTGTYTATPDPL
                     SGEAPQRPKGAQPFPKEMPRGFQEDIMGSPGCHVESP"
     gene            complement(1953..3128)
                     /locus_tag="VEV43_11495"
     CDS             complement(1953..3128)
                     /locus_tag="VEV43_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP24189.1"
                     /translation="MRRAASVVGLLAALLAPAAPSPAAPLAERVELIVAFEDGAGPAA
                     RRAAAATAGATVTGRLPALGLQLWSLPSTAVAAVEAAPAIEWAEPDRTLYLFDKPNDP
                     LLSRQWAFDELEFPKAWELEAGATSPVVVAVVDSGVEATHPDLEGRLVEGRDFLAQDD
                     TPDDPNGHGTGVSGIIAANTDNREGIAGMSWGAKVMPLQTCGETGTCSMFAVVQAIVW
                     AAAHDAQVVNLSLGGALPSCGRTLAAAASFAEARGTLLVAASGNSAQNGNPAGYPAAC
                     DGFMAVGATDVLNRWAAFSQYGPHLSVAAPGVAVMTSWSTETSPKTSRGYAIVDGTSF
                     AAPHVSGLAALLWSRHPDWTPAQVRARIEQSAIDLGPEGHDPWYGYGRIDLRRALAE"
     gene            complement(3125..5815)
                     /locus_tag="VEV43_11500"
     CDS             complement(3125..5815)
                     /locus_tag="VEV43_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24190.1"
                     /translation="MGRARGGRAAASLVVLGIVVGQALAASPPAARAQTGITLKAPCP
                     ALGGAFNRWAPLSTPGRLAHVASFGQAPCTFVGTTSDGAVYRTTDARAWSAVVALDDL
                     ETVAGVAAEEMPVGTAFVFGTPEDALLGSDGTHPRAAGMYVTRDFGASFRAIPDLAGF
                     SVSAVAAAPSDPQVLYAAASPVRGVPVPLTLKSSDFGRTWIPLPGSLPVRPTRIAVDA
                     RLPDVVWANSTVDGTTPSGGVWRSLDGGLTFDRVRDDTVLDFDAAPVPGGGSRVDMAT
                     PGGLVRTRDNGTTFRTVTTESVTAVTHERFAPDALMAVVSARAARSVTAGRTFKASPG
                     LGDLAGCAVTDLTRNEEFPSHFLLSLAGCDAAGHYLYRSDGRDLAGIEDLGGDVESSL
                     VPSFERLPRTEMPILRVVDLPITSPVNPSSGSIAFDGEHLYYTNNDTNHQIHVSTTTG
                     DHVRTLSLDEGYNIRTLTYDPHLDKLWAAIHLPIDPSYLSVYMYLVDPRTGAATQMFK
                     SPLDAQTTLSMDPTEGVFRSYAHHGYQVHEISRSGKVVESCMVPGFPVDPNVSTNPDR
                     NHPEMPAPGFATGLAVGGGRMYLQLEDDRTIYHVSKDCELLSVFEHRRFAESSGGGLE
                     NDQMACDTVTFGQPAIWIRDADVNAAYAYAVPNGYCPLDSKVTMTPPKVTAKAGDEAR
                     ACALLLGDGPSDDVIPVPGAELTFFAGDAPVAAAVTDGDGFACASFRAPPAPARSLPL
                     EAAFFGNVSFRPSSGAGLLETFTPMAPDPKAPVPPPPPPEEPQVVVIPPPLPPPPVQA
                     PVPGGPHAPKPPQQPNPQPQQQPQVQAQAGLARQQQQQTQLAFAFSADESPLLNEEYA
                     MSERGDRNLRLSFQGAAALVMLAFGWCMTRTSYAFRKVRR"
     gene            6051..>6875
                     /locus_tag="VEV43_11505"
     CDS             6051..>6875
                     /locus_tag="VEV43_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP24191.1"
                     /translation="MRNAPAYEVRNLCKVYRDPKVVANDGVSWSVARGEAFGLLGPNG
                     AGKTTLVRQLVGLLRPTSGEIRLFGELVFGGRGPERRVARCVAYLPQGATALGELKVA
                     EAISYTGMLRGLGKAAATSDAEQLMDTLSLGDLAGRQLRKLSGGQRRLVQIGMTLVGR
                     LPVLILDEPTADIDPALRTKIWDLISDRARGGAAVILVTHDVAEAEHVLDRVAIMGRG
                     KVVAAGTPGELKARLSHRTRVEVVVAEDATVDPQALVEHVPGETRVRCKHLSAWVPA"
BASE COUNT          989 a         2416 c         2529 g          941 t
ORIGIN      
        1 gcgaaggtgt cggccctcgg ccgcgcgccg ttgttcatcc cggagcagaa gaaggtggcc
       61 gagctcctcc gtgagatgca ggagcagcac gtgcacatgg cgatcgtcgt cgacgagtac
      121 ggcgggacgg ccgggctcgt cacgatcgag gacctgatcg aggagatcgt cggcgagatc
      181 gtggacgagt acgaccagga ggagccgctg gtcgagcccg tcgacgagga ccgcatccgc
      241 gtcgacgcga agatgccgat cgacgaggtc aacgagctgc tcgaggtcga cctcccgcac
      301 gacgagtggg acacggtcgg gggcctcgtg ttcgggctca cgggacgcgt cccgcaggtc
      361 ggcgagtccg tgcgcttcga ctcgctcgag ttccggaccg agcgcgtcac cggccggcgg
      421 gtgcagaagg tcttgataac gaagaccccc gcccccgagc agggcgacga cgattagcga
      481 gccgttcagg tccgggttcg tcgcggtcgt aggccgcccg aacgtcggca agtccacgct
      541 cgtcaacgcg ctcgtcggca cgaaggtcgc gatcacgtcc gacaagccgc agacgacccg
      601 cagcgcgatc cgcggcatcc tcgaccgcga cggggtccag gtcgtgctcg tcgacacgcc
      661 cggctatcac aagccgcgca cgctgctggg gcagaggctc aacgacctcg tccgcgcgac
      721 gtggaaggac gtcgacctgg ctttgttcgt cgtcgacggg aagtccgggg tcggccgcgg
      781 ggacgagaag gtcgccgcgg acctcgctgc ggcgcaatgc ccgacgttct gcatcgtgaa
      841 caaggtcgac gctatgggcc gcgacgacgt cgcggtcgct ctcacgcaag ccgcggcgat
      901 acgcgacttc gacgagttcg tgccggtctc ggcccggacg ggcgagggcg tcgagctgct
      961 cgagggcctc gtcaccgccc gcatgcccga ggggccgatg tactacccgc ccgggactcg
     1021 gaccgaccag cctcctccgg ccttcgtcgc cgagctcgtg cgcgagaagc tgctggcccg
     1081 gacccgcgag gagctgccgc attcgatcgc ggtcgtgacc gaggacttcg aggagcgcga
     1141 cgacggcctg ctcgagatcc gcgcgacggt gttcgtcgag cgcgagtcgc agaaggggat
     1201 cgtgatcggc aagggcggcg agacggtgaa ggccgtcggg accgagacgc ggctcgagat
     1261 cgaggccctg ttcggtacga aggtgttcct ggagctgcgg gcgaaggtcg agaaggactg
     1321 gcagcggcgc gcccacgcgc tcgagcgtct gggcttcggg ggctagggac tctcgacgtg
     1381 gcagccgggg ctccccatga tgtcctcctg gaatcccctc ggcatctcct tcgggaacgg
     1441 ctgcgcacct ttcggccgtt ggggcgcctc gccggacagc gggtccggtg tcgccgtgta
     1501 ggtcccggtc ccgaagcacg acacgttcat caggttgttc gccgtcaccg tggcccactc
     1561 gtagccgtcg cccgcaggac ccccgtcctg ctcgaggtcc cacctccatt cgatgcggag
     1621 ctccttgccc ccgaacttcg tgtggacgaa cgcgcccata cacacgcact ccgtgacgcc
     1681 caccggcggg acgagcgcga agtccccggg cccgagctct cgtacgtaga cctcggcccg
     1741 gcaccccttg caccgccgcc gcacctccag aacgagctgc gtgccgccgg gccggtcctc
     1801 gacgacggcg tcgacggagt acgcgacgcc gtcgcggtcg cgcgcgagga agtgcgaacg
     1861 gtagacgtcg gcggtgaacg tgccggcgtc cgacgcggga gcgacgacca gcggcatcag
     1921 gcccgagatc gcgagcagga gcgcgacgcg cttcattcgg ccagcgccct tctgaggtcg
     1981 atccggccgt agccgtacca cgggtcgtgg ccctcgggcc cgaggtcgat cgcgctctgc
     2041 tcgatgcggg cgcgcacctg cgcgggggtc cagtccgggt ggcgcgacca cagcaacgcc
     2101 gcgaggcccg agacgtgcgg ggccgcgaac gaggtcccgt cgacgatcgc gtagccgcgg
     2161 ctggtcttcg gcgaggtctc ggtcgaccac gatgtcatca ccgcgacccc gggggccgcg
     2221 accgacaggt gcggaccgta ctgagagaac gcggcccagc gattcagcac gtcggtcgcg
     2281 ccgacggcca tgaacccgtc gcacgccgct gggtagcccg cgggattccc gttctgggcc
     2341 gagttgcccg acgccgcgac gagcagcgtc ccgcgggcct cggcgaacga tgcggcggcg
     2401 gccagcgtgc ggccacagct cggaagcgcg ccgcccagcg acaggttcac gacctgggcg
     2461 tcgtgcgcgg cggcccagac gatcgcctgc acgacggcga acatcgagca cgtcccggtc
     2521 tccccgcacg tctgcagcgg catgaccttc gccccccacg acatgcccgc gatcccctcg
     2581 cggttgtcgg tgttcgccgc gatgatcccc gagactccgg tcccgtggcc gttcgggtcg
     2641 tcgggcgtgt cgtcctgggc gaggaagtcg cgtccctcca cgagccggcc ctcgagatcc
     2701 gggtgggtcg cctctacgcc gctgtcgacg accgcgacga ccaccggcga cgtcgccccc
     2761 gcctccagct cccaggcctt cgggaactcg agctcgtcga acgcccactg gcgggaaagc
     2821 aacgggtcgt tcggcttgtc gaacaggtac aaggtgcggt cgggctcggc ccactcgatc
     2881 gccggcgccg cttcgacggc cgcgactgcg gtcgacggga ggctccagag ttgcagcccg
     2941 agcgcgggaa gccgtccggt cacggtcgcg cccgcagttg cagcagcggc acgacgagcg
     3001 gccgggcccg ccccgtcctc gaacgcgacg atcaactcga cccgttcggc caggggggcc
     3061 gccggagaag gggccgcggg ggcgagcaac gccgcgagga gccccacgac ggaggcggcg
     3121 cgcctcaccg gcgcaccttc ctgaacgcat acgaggtccg cgtcatgcac cagccgaacg
     3181 cgagcatgac gagggccgcc gcgccctgga acgacagccg caggttgcgg tcgccgcgct
     3241 cgctcatcgc gtactcctcg ttcagcaacg gcgactcgtc cgcggagaac gcgaacgcga
     3301 gctgcgtctg ctgctgttgc tgacgcgcca gccctgcctg ggcctggacc tgcggctgct
     3361 gctgcggttg ggggttcggc tgctgcggcg gcttcggcgc gtgcgggccg cccggcaccg
     3421 gggcctggac cggcggcggc ggcagcggcg gcgggatcac gaccacctgc ggctcctcgg
     3481 gaggaggcgg aggcggtacc ggagccttcg ggtcgggcgc catcggcgtg aaagtctcga
     3541 gcagcccggc ccccgacgag ggacggaacg agacgttgcc gaagaacgcg gcttccaaag
     3601 gcaaggagcg cgcgggcgcc ggtggagcgc ggaacgacgc acacgcgaag ccgtcgccgt
     3661 cggtcaccgc cgcagcgacc ggtgcgtcgc ccgcgaagaa cgtgagctcc gcgcccggga
     3721 cggggatgac gtcgtcgctg ggcccgtcgc cgaggagcag cgcacacgcc ctcgcttcgt
     3781 cgccggcctt cgcggtgacc ttcggaggag tcatcgtgac cttgctgtcc agggggcagt
     3841 agccgttcgg caccgcgtac gcgtacgcgg cgttgacgtc cgcgtcccgg atccagatcg
     3901 ccggctgtcc gaacgtcacc gtgtcgcacg ccatctggtc gttctcgagg ccgcccccgg
     3961 acgactcggc gaacctccgg tgctcgaaca ccgacaggag ctcgcagtcc ttgctcacgt
     4021 ggtagatcgt gcggtcgtcc tcgagctgca ggtacatgcg accgccgccg acggcgaggc
     4081 cggtcgcgaa ccccggcgcg ggcatctcgg ggtggttgcg gtcggggttc gtcgagacgt
     4141 tcgggtcgac ggggaagccc gggaccatgc agctctcgac gaccttgccg gacctcgaga
     4201 tctcgtggac ctggtagccg tggtgcgcgt acgaccggaa cacgccttcg gtcgggtcca
     4261 tcgacagcgt cgtctgggcg tcgaggggcg acttgaacat ctgggtcgcg gcccccgtgc
     4321 ggggatcgac caggtacatg tagacgctca ggtacgaggg gtcgatcggc aggtggatcg
     4381 ccgcccacag cttgtcgagg tgagggtcgt aggtgagcgt gcggatgttg tagccctcgt
     4441 cgagggagag cgtccgcacg tggtcgccgg tcgtggtcga cacgtggatc tggtggttcg
     4501 tgtcgttgtt cgtgtagtag aggtgctccc cgtcgaacgc gatcgagccg gacgacgggt
     4561 tcacaggcga agtgatgggc agatcgacga cacgcaggat cggcatctcg gtgcgtggga
     4621 gcctctcgaa gctcgggacg aggctcgact cgacgtcgcc gccgaggtcc tcgatgccgg
     4681 cgaggtcgcg gccgtcgctc cggtacaggt agtggccggc ggcgtcgcaa ccggcgagcg
     4741 acagcaggaa gtggctcggg aactcctcgt tgcgcgtgag gtcggtgacc gcacagccgg
     4801 cgaggtcgcc gagccccggc gatgccttga aggtccgccc ggcggtgacc gaacgggctg
     4861 ctcgcgcgct cacgacggcc atgagcgcgt ccggcgcgaa ccgctcgtgc gtcaccgcgg
     4921 tcaccgattc ggtcgtgacg gtgcggaacg tcgtgccgtt gtcgcgcgtg cggacgaggc
     4981 ccccgggcgt ggccatgtcg acgcggctcc cgccgccggg cacgggcgcg gcgtcgaagt
     5041 ccaggactgt gtcgtcccgc accctgtcga atgtgaggcc cccgtcgagc gaccgccaca
     5101 cgcctccgga cggcgtcgtc ccgtcgacgg tgctgttggc ccagacgacg tcgggcaggc
     5161 gtgcgtcgac ggcgatccgc gtggggcgca cgggaagcga ccccggcaac gggatccacg
     5221 tgcgtccgaa gtcggagctc ttcagcgtga gcgggaccgg gacgccgcgc acgggcgacg
     5281 cggccgcata caggacctgg ggatcgctgg gggccgcggc cacggccgac acggagaagc
     5341 ccgcgaggtc ggggatcgcg cggaacgacg cgccgaagtc ccgggtgacg tacatgcccg
     5401 cggcgcgggg gtgcgtgccg tcggaaccga gcagggcgtc ttcgggcgtg ccgaagacga
     5461 acgccgtccc gaccggcatc tcctcggcgg cgaccccggc gacggtctcg aggtcgtcga
     5521 gggcgacgac tgccgaccac gcccgcgcgt cggtggtgcg gtagacggcg ccgtccgacg
     5581 tggtgccgac gaacgtgcag ggggcctggc cgaagctggc cacgtgggcg agccggccgg
     5641 gggtgctcag gggggcccac cggttgaagg ccccgccgag cgccgggcac ggcgccttca
     5701 gcgtgatccc ggtctgggcg cgggccgccg gcggcgacgc cgccagggcc tgcccgacga
     5761 cgatcccgag cacgacaagc gaagcggccg ccctgccccc acgtgcccgc cccacgccac
     5821 tccttttcgt aggcgccgtc tccgtcagac acacgcagtc cccgctgcga ggtaacggag
     5881 tcttcgccgc cagggcgggg ccgcctcccg cggctgccac gaggcgtcag cagggcgacg
     5941 catagcgacc ggccccccgg gctgcggcgt gtgccctgcg gtccggccga ggccgccccg
     6001 cctgtgccct gcggtccggc cgaggccgcc ccgcgctacc ctcgccgcag atgcgcaacg
     6061 ctcccgccta cgaggtccgc aacctctgca aggtctaccg ggaccccaag gtcgtcgcca
     6121 acgacggcgt ctcgtggtcc gtcgcccgcg gcgaggcctt cggcctcctc ggtccgaacg
     6181 gcgcgggcaa gaccacgctc gtccggcagc tcgtcggcct cctgcgcccg acctctggcg
     6241 agatcaggct gttcggcgag ctcgtcttcg gcggacgggg gccggagcgc cgcgtcgccc
     6301 gctgcgtcgc gtacctgcct cagggcgcga cggctctcgg cgagctgaag gtcgcggagg
     6361 ccatctccta caccggcatg ctgcgggggc tgggcaaggc cgccgccaca agcgacgcgg
     6421 agcagctcat ggacacgctg tctctcggcg acctggcggg ccgccagctc cgcaagctct
     6481 cgggcggcca gcgccgcctc gtgcagatcg gcatgacgct cgtcgggcga ctgccggtgc
     6541 tgatcctcga cgagccgacg gcggacatcg acccggcgtt gcgcacgaag atctgggacc
     6601 tgatctcgga ccgcgcccgc gggggtgcgg ccgtgatcct cgtgacgcac gacgtagcgg
     6661 aggccgagca cgtgctcgac cgcgtcgcga tcatgggccg cggcaaggtc gtcgcggccg
     6721 gcacgccggg cgagctgaag gcgcggctct cgcatcgcac cagggtcgag gtcgtcgtcg
     6781 ccgaggacgc cacggtcgac ccgcaggccc tggtcgagca cgtgcccggc gagacgcgcg
     6841 tgcgctgcaa gcacctgagc gcgtgggtcc ccgcc
//
LOCUS       DASQDO010000412         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_838904, whole genome shotgun sequence.
ACCESSION   DASQDO010000412 DASQDO010000000
VERSION     DASQDO010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838904"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VEV43_11510"
     CDS             <1..268
                     /locus_tag="VEV43_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24192.1"
                     /translation="KILKNGEDVGELALTNLELGLVLKDDDPKKGEKHLREAVRLYTD
                     AEHFLRAARAHRVLGDMLMEQGREGEACKVFRAGLWVLENFECP"
     gene            complement(359..643)
                     /locus_tag="VEV43_11515"
     CDS             complement(359..643)
                     /locus_tag="VEV43_11515"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYP24193.1"
                     /translation="MAELLEDEEIEQRLDELGDWEREGDGIQKVFEFDDFTTAIKFVN
                     DVAKLADRYDHHPDIDIRWNKVKLLLSTHSEGGLTPLDFDVAGEIEQSIR"
     gene            734..1747
                     /locus_tag="VEV43_11520"
     CDS             734..1747
                     /locus_tag="VEV43_11520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYP24194.1"
                     /translation="MDYGIGIGGPLQMIGAMAKAAEDAGFESCWVAETTHSAFIPAAA
                     AIQATSKIKIGTAIALAFPRSPTITAMEAADLDELSGGRFVCGLGSQVKGVNERRFSV
                     KFEHPAPKLREYAGVLRTVWGANRGEDRVFEGRFYNVTMPVFGGKRMPPPRDVPIYFA
                     AVGPLMARICGEVADGLLGHPLASTKYLKEVVRPAVAEGAARAGRKADECNLTASPMI
                     SISDDVDLARREVKLQIAFYATTRTYTKILELHDRAHLVPDLRAAFDARDRKAMVELV
                     DDELCDAIAAAGTVDEVRDRVKAWDGVADRLLMGGPWYGPSFERMVENGQAILETFGS
                     PPA"
     gene            complement(1753..>2215)
                     /locus_tag="VEV43_11525"
     CDS             complement(1753..>2215)
                     /locus_tag="VEV43_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24195.1"
                     /translation="FATLWATKETGFTSADVARYLDARAPAVTDRATEVMDLLLNYDA
                     ATIDDVGDRLIELSTGNFADDYADLVGGGNLEKALREASASSRGQILDGPDVYFRDPD
                     EAVSIVTVTQTAQSESNPTGITTEYVFRIILLRTGGEWKADDVKILSTNEG"
BASE COUNT          341 a          740 c          767 g          367 t
ORIGIN      
        1 gaagatcctg aagaacggtg aggacgtcgg cgagctggcg ctcacgaacc tagagctcgg
       61 gctcgtccta aaggacgacg atcccaagaa gggcgagaag cacctgcgcg aggccgtgcg
      121 gctctacacc gacgccgagc acttcctgcg cgcggcgcgg gcgcatcggg ttctcggcga
      181 catgctgatg gagcagggcc gcgagggcga ggcttgcaag gtcttccggg ccgggctctg
      241 ggtcctcgag aacttcgagt gcccctgacc gggcagaaag cagcgcctac cgtcgccgaa
      301 ggcgacgaca ccgagcccgg ctaggtcgcg ttagggcgcg atcgtctcgg cgatcgcgct
      361 atctgatcga ttgctcgatc tcgcccgcca cgtcgaagtc gagcggcgtc aggcccccct
      421 cggagtgggt cgatagcaac agcttcacct tgttccaccg gatgtcgatg tccgggtggt
      481 ggtcgtagcg gtccgcgagc ttggcgacgt cgttcacgaa cttgatcgcg gtggtgaagt
      541 cgtcgaactc gaagaccttc tggatcccgt cgccctcgcg ctcccaatcg ccgagctcgt
      601 ccaggcgttg ttcgatttct tcgtcttcga gcagttccgc caccgtgcct cctactagtc
      661 gatagctgcc acgcaatcgg ggcgaccata ccgcggcggg ggacgccgtt tcgcgccgtc
      721 tatagtcccg gccatggact acgggatcgg catcggcggg cccctgcaga tgatcggcgc
      781 gatggccaag gcggccgagg acgcgggctt cgagtcgtgc tgggtcgcgg agacgacgca
      841 ctccgcgttc atccccgcag ccgccgccat ccaggcgacg tcgaagatca agatcggcac
      901 cgcgatcgcc ctcgcgttcc cgcggtcgcc gacgatcacc gccatggagg cggccgacct
      961 cgacgagctc tcgggcgggc gtttcgtctg cgggctgggc tcccaggtca agggtgtgaa
     1021 cgagcgccgc ttctcggtga agttcgagca cccggccccc aagctgcgcg agtacgcggg
     1081 cgttctgcgg accgtgtggg gcgcgaaccg cggcgaggac cgcgtgttcg agggtcgctt
     1141 ctacaacgtg acgatgccgg tcttcggggg caagaggatg ccgcccccgc gcgacgtgcc
     1201 gatctacttc gccgccgtcg gcccgttgat ggcgcgcatc tgcggcgagg tagcggacgg
     1261 cctcctcggc cacccgctcg cgtcgacgaa gtatctgaag gaggtcgtgc gacccgcggt
     1321 cgccgagggc gccgcgaggg ccggacgcaa ggccgacgag tgcaacctga cggcgtctcc
     1381 gatgatctcg atctcggacg acgtcgacct ggcccggcgc gaggtgaagc tccagatcgc
     1441 cttctacgcg acgacgagga cgtacacgaa gatcctcgag ctgcacgatc gcgcccacct
     1501 cgtccccgat ctgcgcgctg cgttcgacgc ccgcgacaga aaggcgatgg tcgagctcgt
     1561 cgacgacgag ctctgcgacg cgatcgcggc agcggggacg gtcgacgagg tccgcgaccg
     1621 cgtgaaggcg tgggacgggg tcgccgaccg gctgctgatg ggcgggcctt ggtacgggcc
     1681 gagcttcgag cgcatggtcg agaacggcca ggcgatcctc gagacgttcg gttccccgcc
     1741 cgcctagccc gcctagccct cgttcgtgct caggatcttc acgtcgtccg ccttccactc
     1801 cccaccggta cggagaagga tgattcggaa gacgtattcg gtcgtgatcc ccgtcgggtt
     1861 gctctccgac tgtgccgtct gcgtcaccgt gacgatcgac acggcctcgt cggggtcgcg
     1921 gaagtagacg tcgggcccgt cgaggatctg cccccgcgac gacgccgacg cctcgcgtag
     1981 cgccttctcg aggttcccgc cgccgactag gtccgcgtag tcgtcggcga agttcccggt
     2041 cgacagctcg atcaagcggt ccccgacgtc gtcgatcgtc gccgcgtcgt agttgaggag
     2101 caggtccatg acctcggtcg cccggtccgt gacggccggg gcgcgggcgt cgaggtagcg
     2161 agcgacgtcg gcggacgtga acccggtctc cttcgtcgcc cacagggtgg cgaag
//
LOCUS       DASQDO010000413         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1145109, whole genome shotgun sequence.
ACCESSION   DASQDO010000413 DASQDO010000000
VERSION     DASQDO010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145109"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VEV43_11530"
     CDS             complement(<1..264)
                     /locus_tag="VEV43_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYP24196.1"
                     /translation="MPRRLRHGESVTLVEHLDELRTRLIVSLLVLGLGVAIAFPFHER
                     IVEWLVDPLPDDRQLVTFGVTEPFVTSFKVSLAAGFLLAFPVLL"
     gene            complement(289..543)
                     /gene="tatA"
                     /locus_tag="VEV43_11535"
     CDS             complement(289..543)
                     /gene="tatA"
                     /locus_tag="VEV43_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYP24197.1"
                     /translation="MPAGLGLPELLIIFALLLLLFGAKRLPEMGRSLGRGMREFKEAV
                     TGSDDAPERGAELPSRATVTPPPPPPAAPAPPSETERVSS"
     gene            660..1445
                     /gene="sufC"
                     /locus_tag="VEV43_11540"
     CDS             660..1445
                     /gene="sufC"
                     /locus_tag="VEV43_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HYP24198.1"
                     /translation="MSLLEIKNLHVALEDGTPIVRGVDLTIESNEVHAIMGPNGSGKS
                     TLAYALMGHPAYEITEGEILLDGVDVVGMEADERAQRGMFLAFQYPYSVPGVTVASFL
                     RSAVNAVRKARNGGEDNPIAIKEFRTQMTGAMEELKVPKEMAGRYLNEGFSGGEKKRV
                     EILQMAMLQPRVAVLDETDSGLDIDALKVVAEGVQRLVGPEMGALVITHYQRILNYIK
                     PDFVHVFVDGRIVAQGGPELAERLEAEGYEAFIPAAATVGGDL"
     gene            1445..>2021
                     /locus_tag="VEV43_11545"
     CDS             1445..>2021
                     /locus_tag="VEV43_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HYP24199.1"
                     /translation="MAITETEVVQGIGSDYEVKYGFSVPEDYFFKSGKGLSHELVDAI
                     SSHKDEPEWMRKFRHQSLDYFYARPMPAWGGDLSTIDFEDIYYYIRPTENQANSWEDL
                     PADIKDTWDKLGIPEAEKKYLAGVGAQYESEVVYHKLQEELENKGVIFLDMDSALREH
                     EDIVRAHMGTIIPQNDNKFAALNSAVWSGGSF"
BASE COUNT          384 a          650 c          712 g          275 t
ORIGIN      
        1 gaggaggacg gggaacgcga gcaggaagcc cgccgcgagg ctgaccttga acgaggtgac
       61 gaacggctcg gtgacgccga aggtgacgag ctggcgatcg tcggggagcg ggtcgacgag
      121 ccactcgacg atgcgctcgt ggaacgggaa ggcgatggcg accccgaggc cgaggacgag
      181 cagagagacg atcagccggg tgcgaagctc gtccaggtgc tcgacgagcg tgacgctctc
      241 gccgtgccgg agccgacgcg gcaagcggat agccaccggc gcggagtgct agctcgagac
      301 gcgctcggtc tcggacggcg gcgccggcgc ggccggggga ggcggcggcg gcgtcacggt
      361 ggcgcgcgag ggaagctcgg caccccgctc gggggcgtcg tcgcttccgg ttaccgcctc
      421 cttgaactcg cgcatcccgc gaccgagcga acggcccatc tcgggcagtc gcttcgcccc
      481 gaagaggagc aggaggaggg cgaagatgat gagcagctcg ggcaggccga gtcctgcggg
      541 catggccgaa tcgtagcacc cccacccggc ccggaagccc aggccccgcc gctagactgt
      601 gtgagtcacg agccgcccgg ctccgccaga gaatcccttc agggaggctg ttacaaacct
      661 tgtcgctcct cgagatcaag aacctgcacg tcgcgctcga agacgggacc ccgatcgtcc
      721 gcggcgtcga cctcacgatc gaatcgaacg aggtgcacgc gatcatgggc ccgaacggct
      781 ccggcaagtc gacgctcgcc tacgcgctca tgggccaccc ggcctacgag atcaccgagg
      841 gcgagatcct gctcgacggc gtcgacgtcg tcggcatgga ggcggacgag cgcgcgcagc
      901 gcggcatgtt cctcgccttc cagtacccgt actcggttcc gggggtcacc gtggcgagct
      961 tcctccgctc ggccgtgaac gcagtccgca aggcgcgaaa cggcggcgag gacaatccga
     1021 tcgcgatcaa ggagttccgc acccagatga ccggtgcgat ggaggagctg aaggttccga
     1081 aggagatggc ggggcgctac ctgaacgagg ggttctcggg cggcgagaag aagcgcgtcg
     1141 agatcctcca gatggcgatg ctgcagccgc gcgtcgccgt cctcgacgag accgactcgg
     1201 ggctcgacat cgacgccctc aaggtcgtgg ccgagggcgt ccagcgcctg gtcgggcccg
     1261 agatgggcgc gctcgtcatc acgcactacc agcggatcct gaactacatc aagcccgact
     1321 tcgtgcacgt cttcgtggac gggcgaatcg tcgcacaggg gggcccggag ctcgcagagc
     1381 ggctcgaggc cgagggctac gaggccttca tcccggcggc ggcgacagtg ggaggagacc
     1441 tgtaatggca atcaccgaga ccgaggtcgt ccaggggatc gggtccgact acgaggtcaa
     1501 gtacgggttc tccgtcccgg aggactactt cttcaagtcc ggcaaggggc tctctcacga
     1561 gctcgtcgac gcgatctcga gccacaagga cgagcccgag tggatgcgga agttccgcca
     1621 ccagagcctc gactacttct acgcgcggcc gatgccggcg tggggcggcg acctctcgac
     1681 gatcgacttc gaggacatct actactacat ccggccgacc gagaaccagg cgaactcctg
     1741 ggaggacctg ccggccgaca tcaaggacac ctgggacaag ctcgggatcc ccgaggcgga
     1801 gaagaagtac ctcgccgggg tcggcgccca gtacgagtcc gaggtcgtct accacaagct
     1861 ccaggaggag ctcgaaaaca agggcgtcat cttcctggac atggactcgg cgcttcgcga
     1921 gcacgaggac atcgtgcgcg cgcacatggg gacgatcatc ccgcagaacg acaacaagtt
     1981 cgcggccctg aactcggcag tctggtcggg cggatccttc a
//
LOCUS       DASQDO010000414         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_349396, whole genome shotgun sequence.
ACCESSION   DASQDO010000414 DASQDO010000000
VERSION     DASQDO010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349396"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VEV43_11550"
     CDS             complement(<1..951)
                     /locus_tag="VEV43_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HYP24200.1"
                     /translation="MNLADLAVIVLGGLAAYRGFRHGLLGQFFEIGGGFLGLVLGVTL
                     AGSIADAFTDEPGMQALLISLLTVFVMLSLGQTLGHLVGARFGRAAKNSKLGGVNQGL
                     GAGFGIFVTLLSYWLIGSLLVHGPSREVAKLMSKSVILKELNAISKPPDVLAYLRQYL
                     DTAGFPQVFAGLPRPIGEPVDLPSDKAARAAARKVQASTVRIVVPACGGQQLGTGWIG
                     APDTVVTNAHVVAGGGDVEVQDQSGSHTGRVVLFDPGTDVAIVHVTGLTGRPLDLETS
                     ELERGTPGATLGFPGSNAPEMDVDPAAVQDSYPARGRDIYG"
     gene            complement(952..1332)
                     /locus_tag="VEV43_11555"
     CDS             complement(952..1332)
                     /locus_tag="VEV43_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24201.1"
                     /translation="MIDLHRYVAYSIPTLFALLTLGAIWTYLRNREPGDWYWNLLAAV
                     QIVLGVQLVVGVVLFALGGRPQSNGPNWLHYVYGALFPIGVLYFAHRYAKKHQGVEMI
                     IFGIACFVSFGLTFRALQTGLGID"
BASE COUNT          262 a          519 c          534 g          206 t
ORIGIN      
        1 gccgtagatg tcgcggcccc tggccgggta gctgtcctgc acggccgccg ggtcgacgtc
       61 catctccggc gcgttcgagc ccgggaagcc gagcgtcgcc cccggcgtcc cgcgctctag
      121 ctcggacgtc tcgaggtcca gcggccggcc ggtgaggccg gtgacgtgga cgatcgccac
      181 gtcggtgccg gggtcgaaca gcactacccg tccggtatgc gatcccgatt ggtcctggac
      241 ctcgacgtcg ccgccccccg ctacgacgtg cgcgttcgtc acgacggtgt cgggagcgcc
      301 gatccatccg gtccccagct gctgcccccc gcacgcgggg acgacgatcc gcaccgtgga
      361 cgcctgcacc ttgcgcgcgg ccgcccgcgc ggccttgtcg gacgggaggt cgacgggctc
      421 gccgatggga cgcggcaagc cggcgaacac ctgcgggaag ccggcggtgt cgaggtactg
      481 gcgcaggtag gcgagcacgt cgggcggctt cgagatcgcg ttgagctcct tgaggatcac
      541 ggacttgctc atgagcttcg ccacctcgcg cgacggcccg tgcaccagca aggagccgat
      601 cagccagtac gagagcagcg tcacgaagat gccgaagccc gctccgaggc cctggttcac
      661 gccgccgagc ttggagttct tcgccgcccg cccgaaccgc gccccgacga ggtgcccgag
      721 cgtctggccg agcgacagca tgacgaagac cgtcagcaac gagatcaaca gcgcctgcat
      781 gccgggctcg tccgtgaacg cgtcggcgat cgagccggcc agcgtgacgc cgaggacgag
      841 cccgaggaac ccgccgccga tctcgaagaa ctggccgagg agaccgtgcc tgaacccgcg
      901 gtacgcggcc aggcccccga ggacgatgac cgcgaggtcg gcgaggttca ctcagtcgat
      961 gccgaggccg gtctgcagag cccggaacgt gaggccgaac gacacgaagc aggcgatgcc
     1021 gaagatgatc atctcgaccc cctggtgctt cttggcatac ctgtgcgcga agtagaggac
     1081 cccgatcggg aacagcgccc cgtagacgta gtggagccag ttcggaccgt tcgactgcgg
     1141 acgcccgccg agcgcgaaca gcacgactcc gacgacgagc tggacgccga ggacgatctg
     1201 gacggcggcg agcaggttcc agtaccagtc acccggctcg cggttgcgca ggtaggtcca
     1261 gatcgcgccc agggtgagca gcgcgaacag cgtcgggatc gaatacgcga cgtaacgatg
     1321 gaggtcgatc acggggcgag gttatcccct gtcagggaca ggccgacggg ccggacgtgc
     1381 ccgcgcagac cacgtcgcgg tgtagctgca gcgacaggtc cagcgcatcc ggcgggtcgt
     1441 tcgtcgggtc gaggtcgcct aagaggttgt gcagctccca cgggtcctcg acttcgcgca
     1501 caggtatgcc aagaagcacc a
//
LOCUS       DASQDO010000415        17411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1084545, whole genome shotgun sequence.
ACCESSION   DASQDO010000415 DASQDO010000000
VERSION     DASQDO010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084545"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..795
                     /locus_tag="VEV43_11560"
     CDS             109..795
                     /locus_tag="VEV43_11560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HYP24202.1"
                     /translation="MKDRLPVGREKVEAVRSMFDSIAPRYDLVNRVMTFGMDTGWRRK
                     SIAALNLRPGDLVLDVACGTGDFCRELEGAGHRVVGVDFSMGMLREARTDAPLFQGDA
                     LRLPLRDGSVDAVTCGFALRNVTDVGDLFDELARVVRPGGRIALLEVSQPESRVLRAG
                     HHVYFHKVVPFVGGLLSDRDAYGYLPRSTAYLPPTEDLLARLRAAGFRDAAARHMGLG
                     AVQLLSGTRA"
     gene            792..1931
                     /locus_tag="VEV43_11565"
     CDS             792..1931
                     /locus_tag="VEV43_11565"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HYP24203.1"
                     /translation="MNDLLEIAARVDDPVVWLNGGEGMVAAGVAAEIEVGSGEGRFER
                     AAAELHDVFGSLGGRAFAFGSFAFDPLSDDSVLIVPEVVWGPSWVATARGADPDSFLR
                     ERPTLTADRVRYGGSTISEVAWLDAVATAAERIRAGDLAKVVLARDVLVWSETPFDVP
                     LLLARLARRFPGCFTFSCAGLVGATPELLVARSGNDVRSVVLAGTIGRGDGAEDERLA
                     EGLMSSEKDLEEHRFAVESVTGPLGAVCSDLKVEGPLLLRLANAQHISTRIQGVLERD
                     ATALELAGLLHPTAAVCGTPRRTAFETIRSLEGMDRGRYSGPVGWVDSNGDGEWGIAL
                     RCGRFEGARGRLFAGNGIVGASEPEAELEETRVKLRAMMSALEGA"
     gene            1951..2325
                     /locus_tag="VEV43_11570"
     CDS             1951..2325
                     /locus_tag="VEV43_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24204.1"
                     /translation="MKADGPILAEGERAPGWWARYRFYFAGKVPRGHEDWALRRMSLG
                     RWVAFYLALDLVAWTAAMATSALVTREVDFPGPLWFAWVLVVVLAPVTAVRRRNQAAR
                     SLAPPPADEVLRDIREPVPPRP"
     gene            complement(2322..4028)
                     /gene="menD"
                     /locus_tag="VEV43_11575"
     CDS             complement(2322..4028)
                     /gene="menD"
                     /locus_tag="VEV43_11575"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HYP24205.1"
                     /translation="MGSGLVNARSAGDAAAFCLLDDLYRCGVRHVCLSPGSRSTPLAV
                     AATSDERLRVHVSIDERSSAFLALGIARATGLPAVVVSTSGTAVANFLPAVTEASNAS
                     VPMIVVTADRPPELRGTGANQTIDQVKVFGDAVRWFCEAGVPDDRVPLARYWRSVACR
                     AFAESVARDPGPVHVNVCFREPLTASPEDEVVTDVEGRAGGEPWTAVTRARAVPSPAD
                     VEWLAEKVASVERGLILAGAGDAEAAGVHALAEAAGWPVLAEPSSGLRYGPNAISTYE
                     ALLRAGWAEDHRPDFVLRIGKIGISSVVNGFFRDGPQALVDAHGRWLDPERGADRIVV
                     AEPGLLCAAAAPTAPRRSGWLDEWTAADRAARAAIDAVLDATDEASEPRVARDLAAQA
                     PDGSTLVVAASMPVRDLDWFMAPRDGLRVTGNRGANGIDGFVSTTLGVALASEGPVLA
                     LAGDLSMLHDQNGLLLARNEAVDAVFVVLNNDGGGIFSFLPQAGAVPDFERAFATPHG
                     IDFAQVAALYGCGYSRLETASALPELVSARGLYVVEARTDRAANVDLHRELWAAVGAA
                     LR"
     gene            complement(4010..4897)
                     /locus_tag="VEV43_11580"
     CDS             complement(4010..4897)
                     /locus_tag="VEV43_11580"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HYP24206.1"
                     /translation="MRVRFRRVTERSGVLVRGPQGWGEFSPFPEYGPPATRAWRACAL
                     EAATKPWPAPVRDFVPVNAIVPAVGPEQAHAMTLASGCTTVKVKVAEGDDEARVEAVR
                     DALGPEGRIRIDVNGAWDVDEAAKWIEVLGRYDLEYVEQPVATLEEMAALRKRVDVPL
                     AADESVRTADDPLRVKGLEAADVVVLKVQPLGGVWRCLEVAEAAGLPAVVSSAVETSV
                     GLAAGLALAAALPELPYACGLGTASLLAGDVVADPLVPVDGVLRVCRPGVDDASLQAF
                     ETTGSGHGDEWIERWEAAS"
     gene            complement(4921..5781)
                     /locus_tag="VEV43_11585"
     CDS             complement(4921..5781)
                     /locus_tag="VEV43_11585"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HYP24207.1"
                     /translation="MNVWVEAARPRTLPAAVAPVLVGAGAARVWDTWRFAGALVVALS
                     LQVAVNYANDYFDGVRGVDTADRVGPRRATASGLVQPGQMKVAIGGALAVAGLAGLGL
                     TFAVGPELLLVGVASIAAALAYSGGPRPYASAGLGELFVFTFFGPVATIGTAYVLTEE
                     LLGRAVVASIPVGLVAAAILVVNNVRDISTDAAAGKRTLAVRLGPRRTRHLLAGLVAV
                     ACAVAVGLVATAGRDPSFVFAVLGCGAVLFVPVSNVYRAEGAGLVPALGSLALLHLVF
                     SAQLAVALAR"
     gene            complement(5789..6601)
                     /gene="menB"
                     /locus_tag="VEV43_11590"
     CDS             complement(5789..6601)
                     /gene="menB"
                     /locus_tag="VEV43_11590"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HYP24208.1"
                     /translation="MDWERTRSYDDIVYETAEGIAKITINRPEVRNAFRPQTLFELQD
                     AFNVARDDPGIGVIIFTGAGDKAFCSGGDQRVRGDDGYKDQHGVGRLNVLDLQVQIRR
                     LPKPVIAMVAGYAIGGGHVLHVVCDLTIAAENARFGQTGPKVGSFDGGYGAGLLARMV
                     GHKKAREIWYLCEQYDAQEALQMGLVNKVVPLDRLEEETVAWARRILQMSPLALRLLK
                     AAFNADTDGLAGIQQLAGDATLLYYMSEEAQEGRNAYLEKRTPRFDKFPKRP"
     gene            6661..7791
                     /locus_tag="VEV43_11595"
     CDS             6661..7791
                     /locus_tag="VEV43_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYP24209.1"
                     /translation="MDIPETELVALRWPPGPEMAARLERLWAEGAAVLPLRWDLPAPE
                     IRRLLDLARPAALVEPGGTTRLDDQLPARGALCLATAGSTGPPKLVELSRQALEAAAR
                     LTSAHLGSEGARWLCCLPLDHVAGVMTVVRSRLHGAEPVVLPRFDAAEVAAADADAVS
                     LVPLMLERLLAHGADLTRWRWILLGGAAVRPDLLDAARAAGANVVRTYGMTETCGGVV
                     YDGEPLEGVEVRVEEGIVALRTPTLMEGYRGQAPVRGGWFVTGDAGEWDGRRLRVLGR
                     ADEMIVTGGENVSPAEVEALLASHPNVAEVAVAGAADPEWGQRVVAYVVPAGDAPSLE
                     DLRAHVKGQAAAYKAPRQLVVVDSLPRLPSGKVARASLSSGA"
     gene            7805..8707
                     /locus_tag="VEV43_11600"
     CDS             7805..8707
                     /locus_tag="VEV43_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24210.1"
                     /translation="MRIVFPKRALAGAAVLLLLLGACSDPQGTQPGEAAATADAEDGS
                     GSGTGGDDEDTTAQGGSKNGSGKGGDAKGGSKPDGGGNDGGSGSGGGPRGSGGGGNPG
                     FAGGGGGSGGYFAAGEYVYDQQGFERFCQGPRCSKEKLPDTATITASYESDSVIVTEA
                     RSSDQQTITTTTRHTPGQALITKVVIDFAYGSFNFSQTYEPQPPIESLSFPLEAGKTW
                     RGRWKAQTSGDYRMEVTGVSDGVYRIESVTNFRGDFSGRAQATIWVDGRTRAIVRTDG
                     DVAVASRFGEYTSSFETSLRSAPQ"
     gene            8704..9426
                     /locus_tag="VEV43_11605"
     CDS             8704..9426
                     /locus_tag="VEV43_11605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYP24211.1"
                     /translation="MSAAPTAPARPELPDWDYDGAPGSRDRTRAGIAIVAGVALAGTV
                     LAWLVLGPIGIAAFAALFSGAIVWTVSNSGRGMLRRSGAVPVVPGSAPRLENIVEGLA
                     SKTKLPAPELWLIRDDAPNALVCFTRRPAIAMTSGLVEGYTRTELEAVVAHCFVRLAV
                     TATPLARTMLALSTISSAFGVPVGDRDDVRAAAVTRYPPALASAVTKASPRSGRGASF
                     WFVADDGMHVPAAERSKALLDL"
     gene            9496..10395
                     /gene="pdxS"
                     /locus_tag="VEV43_11610"
     CDS             9496..10395
                     /gene="pdxS"
                     /locus_tag="VEV43_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HYP24212.1"
                     /translation="MSDTTARETGGLRVKRGLAEMLKGGVIMDVTTAEQAKIAEEAGA
                     VAVMALERVPADIRAEGGVVRMADPEKVSEIMESVSIPVMAKCRIGHFVEAQVLQALG
                     VDYVDESEVLTPADEANHVNKWDFDVTFVCGATNLGEALRRIGEGAAMIRSKGEAGTG
                     NVVEAVRHMRSITGTIRKLASLGSEELMSEAKELRAPFDLVREVAETGKLPVVLFTAG
                     GIATPADAALMMQLGADGVFVGSGIFKSGDPATRARAIVDATTHFQDPGIVAKVSHGL
                     GEAMVGINIADIPDADRLAKRGW"
     gene            10401..10973
                     /gene="pdxT"
                     /locus_tag="VEV43_11615"
     CDS             10401..10973
                     /gene="pdxT"
                     /locus_tag="VEV43_11615"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HYP24213.1"
                     /translation="MKVGVLGLQGDFREHARALEDSGATAVVVKTPEQLAGVDALVMP
                     GGESTTIGKLLDRFELLEPLRERAASGMPLYGTCAGLILMANRIEGEQDAPHRLGLMD
                     VAVRRNAYGRQVDSFETDLDVAGLDEPYRAVFIRAPVVERAGAGVEVLASVDGKPVLV
                     RQGTMLGSTFHPELTGDNRVHSMLVGMVRQ"
     gene            10974..11723
                     /locus_tag="VEV43_11620"
                     /pseudo
     CDS             10974..11723
                     /locus_tag="VEV43_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
     gene            11698..12927
                     /locus_tag="VEV43_11625"
     CDS             11698..12927
                     /locus_tag="VEV43_11625"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYP24214.1"
                     /translation="MSSRRSLPDDPRLREVAEQLAATRWAASIVDDAYKLVWVSDEMA
                     NLLGEQDEGKLGYGKHLLEAQIGETWCKVITDASHVSAFEAELPIMIEGTPGGKEALL
                     ETLGGASVGLSDELVTEDMVRRFVEMEPQRPPSVWVNHLEFIQGELPPVKVTEVQIKL
                     YEKDSGEFIGRAFLYGPALAASVLSLVARGEEDMFQRMARLVDPGRRQAAVLFADLEA
                     SSVLSRRLPSSAYFKLVRAITTAMDDQVVKHTGIVGKHAGDGVTAFFLAEDLGSPSRA
                     ARAAIEAGRGMIETARGAAGEIRDETGLLELDDCVINVGIHWGGALYMGQLVTGGRLE
                     ATALGDEVNECARIQETSRAGKILASKALVERLTDDDARALGLDPDSVLYCTIGDIEG
                     ASPKAKRDAGGIPVTPL"
     gene            complement(12924..13424)
                     /locus_tag="VEV43_11630"
     CDS             complement(12924..13424)
                     /locus_tag="VEV43_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24215.1"
                     /translation="MAVATVAAAALAAVPAYALMDSQPDAGVTSYAPTRRTTVYRTEA
                     VTTAETRFRKIPLMKLFVQARGAATIRFSGDFSGGPVEIEILRLPRRQLEPGVAHFTP
                     AGDAESFSYDFVDTRRGGGIECRRYAVRWRSPTGAEVTLNHGSVTIDHRFDDTTRDGL
                     KKTCVD"
     gene            complement(13445..13963)
                     /locus_tag="VEV43_11635"
     CDS             complement(13445..13963)
                     /locus_tag="VEV43_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24216.1"
                     /translation="MRTKLFAAALVTALCLPATAVADHRPWPHASLVTADASEPGSFA
                     GARWATRSSWECVIAFAEGEYGFPSPPVRWVPGTEVAVRIASAERPRRVEARAFLFGD
                     PTTGTPIYGQLEVPSELRRVEVEGETMWEAVLSPPPWPDLYLEVKAEWKRIPRCGERW
                     ATWKWRAGLLPI"
     gene            13962..15191
                     /locus_tag="VEV43_11640"
     CDS             13962..15191
                     /locus_tag="VEV43_11640"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYP24217.1"
                     /translation="MPSNFGCARRFSCVEACSNRPIDERLGAWAHHIEKLQWSAIVLD
                     SEWRFVWASSEMAEFIGEDDGDKLGYGKHIAEAFLSDVWLRTVHPDSQVELFNHLAPY
                     LLHDFARRGRDPKDVLPESFLPLLEGIEPTDPPAMDSISFAYVNPSSDEDLPDYRVNG
                     LFLRLNDDAGELIGWLCVFFMDIRPNLLVLLARGDEQMYERMAKLVDPGPRQAAIMFC
                     DLHGSGTLSRRLPSAAYFKLVRRLWRGIDAAVAEHSGIVGKHAGDGASAFFLVDDLGS
                     PSAAAAAAIRAAARVHEISHGVFEDALESDCLMKVGLHWGGSLYMGQLVPGGRLDVTA
                     LGDEVNEAARIQETAGAGETLASKQLVEQLTTDEAAALGVDVEKLSYRPLAEIEGVPE
                     KAARDAGGLAVTNQLGH"
     gene            15266..15775
                     /gene="ruvC"
                     /locus_tag="VEV43_11645"
     CDS             15266..15775
                     /gene="ruvC"
                     /locus_tag="VEV43_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYP24218.1"
                     /translation="MRVLGIDPGFATTGYGVVERIAGALRPVTIGVVRTPPALPQADR
                     LAHLAGALRGVIHATQPDVVAIERLFFNSNVRTAMSVGQASGVALLAAAEAGLEVVDY
                     TPSEVKLSVAGVGNAAKPQVQAMVAALLKLQAVPKPADAADACALAICHLNRSGLSRA
                     VAAARKRAG"
     gene            15772..16347
                     /gene="ruvA"
                     /locus_tag="VEV43_11650"
     CDS             15772..16347
                     /gene="ruvA"
                     /locus_tag="VEV43_11650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYP24219.1"
                     /translation="MIGFLRGAVAARTTDGCFLEVAGVGYRLACSATTLAALPPEGGE
                     VRLWTHLHVREDALSLFGFATESEQRIFEALLGVNGVGPKVALQVCSAFTPDALRKAL
                     VTDDVAAISSVPGIGKKTAQRMILDLKEKLALPDLEVVGVRPDALSKARSALENLGYS
                     AGEVRVALSEAVPAEEASVEDIVKSALKVLA"
     gene            16347..17372
                     /gene="ruvB"
                     /locus_tag="VEV43_11655"
     CDS             16347..17372
                     /gene="ruvB"
                     /locus_tag="VEV43_11655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYP24220.1"
                     /translation="MTDEVLAKVANPEDVEVDRSLRPRTLVEFVGQPELKEHLAIVLQ
                     AARERNEPAGHLLLSGPPGLGKTSLAHVVANEMDASIRPTSGPALERAGDLAAILTNL
                     EDADVLFIDEIHRLPRAVEEVLYPAMEDFQLDLVIGKGPSAHTIRLDLPRFTLIGATT
                     RAGLITGPLRSRFELAERLDYYPPEDLERIVRRSAAILDVSLDEEGALEIARRSRGTP
                     RIANRLLRRVRDFAQVRANGSIDGDVARDALAVFKVDDKGLDKTDGAILEALVRRFGG
                     GPVGLGTLAVSVGEEPDTIEDVYEPFLMQLGLLRRTPRGRVATEAAFAHVGAPLPKGA
                     AARLFES"
BASE COUNT         2523 a         6190 c         6281 g         2417 t
ORIGIN      
        1 ggcgtttgcc gcgatgctcc cggcgaccgg cgcgctcgtc gggagtcact tccggcgtct
       61 cctgatcgca gaggcgcagg cgcggctcga gagctcggcg agcggctcgt gaaggaccgc
      121 ctgcccgtcg gtcgcgagaa ggtcgaggcc gtccggtcga tgttcgactc gatcgcgccc
      181 cggtacgacc tcgtcaaccg cgtgatgact ttcggcatgg acaccggctg gcggcgcaag
      241 tcgatagccg cgctgaacct tcgtcccggc gacctcgtgc tcgacgtcgc gtgcgggacc
      301 ggcgacttct gccgggagct cgagggggcc gggcaccggg tcgtcggcgt cgacttctcg
      361 atggggatgc tgcgcgaggc gcggacggat gccccgctgt tccagggcga cgcgctgagg
      421 ctgccgctgc gcgacgggag cgtcgacgcc gtcacctgcg ggttcgcgct gcgcaacgtc
      481 acggacgtcg gcgacctctt cgacgagctc gcgcgggtcg tgcggcccgg gggccggatc
      541 gcgttgctgg aggtgtcgca gcccgagtcg cgtgtcctcc gggccggaca ccacgtgtac
      601 ttccacaagg tcgtgccgtt cgtcggcggg cttctgtccg accgggacgc gtacggatac
      661 ctcccgcgct ccaccgcgta tctgccgccg accgaggatc tcctggcgcg cctgcgcgcc
      721 gcgggcttcc gcgacgcagc ggcccggcac atgggcctcg gcgcggtcca gctcctctcc
      781 gggacgaggg catgaacgac ctgctggaga tagccgcgcg cgtcgacgat ccggtcgtgt
      841 ggctcaacgg cggcgagggg atggtcgccg ccggtgtcgc ggcagagatc gaggtcggct
      901 ccggcgaggg ccggttcgag cgcgccgcgg ccgagctcca cgacgtgttc ggctctctcg
      961 ggggccgggc gttcgcgttc gggagcttcg cgttcgaccc gctgtcggac gactcggtgc
     1021 tgatcgttcc cgaggtcgtg tgggggccgt cctgggtcgc gaccgcccgc ggtgcggatc
     1081 ccgactcctt cctgcgggag cgcccgacgc tcacggccga ccgcgtgcgc tacggcgggt
     1141 cgacgatctc ggaggtggca tggctcgacg cggtggcgac ggcggcggag cggatccgcg
     1201 ccggcgacct cgcgaaggtc gtgctcgctc gcgacgtgct cgtgtggtcg gagaccccgt
     1261 tcgacgtgcc gctgctgctg gcgcgtctcg cacggcggtt ccccggatgc ttcacgttct
     1321 cgtgcgcggg gctcgtcggc gcgacgccgg agctcctcgt cgcgcggtcc ggcaacgacg
     1381 tgaggtcggt cgtgctcgcg gggacgatcg gccgcggtga cggtgccgag gacgaacgtc
     1441 tcgcggaggg cctgatgtcg tccgagaagg acctggagga gcatcgcttc gcggtcgagt
     1501 cggtgaccgg gccgctcggt gccgtgtgct cggacctgaa ggtcgagggg ccgctgttgc
     1561 tgcggctcgc gaacgcgcag cacatcagca cgcggatcca gggcgtgctc gaacgcgatg
     1621 cgacggcgct ggagctcgcc ggtctgctgc acccgaccgc ggcggtgtgc gggacgccgc
     1681 gccggaccgc gttcgagacg atccggtcgc tcgaagggat ggaccgaggg cgttacagcg
     1741 gcccggtcgg gtgggtcgac tcgaacggcg acggcgagtg ggggattgcg cttcgctgcg
     1801 gccgcttcga gggtgcgcgg ggcaggctct tcgccgggaa cgggatcgtc ggcgcgtcgg
     1861 agcccgaagc cgagctggag gagacgaggg tcaagctgcg ggcgatgatg tcggcgctcg
     1921 agggggccta gccgctactc tggtggcggg atgaaggcgg acggaccgat cctcgccgaa
     1981 ggcgagcgcg cgccgggttg gtgggcgcgt tatcgcttct actttgccgg gaaggtgccc
     2041 agggggcacg aggactgggc attgcgccgc atgtcactcg ggcggtgggt cgcgttctac
     2101 ctcgcactcg acctcgtcgc ctggaccgcc gcgatggcga cctcggccct ggtcacgcgc
     2161 gaggtcgact tcccgggacc cctctggttc gcatgggtgc tggtcgtcgt tctcgctccg
     2221 gtaacggcgg tgcgacggcg taaccaagcc gcgcgttcgc tcgctccacc tccagcggac
     2281 gaggtcctga gggacatccg cgagcccgtt cctccgcggc cctagcggag cgcggccccc
     2341 acggcggccc acagctcgcg gtgcagatcg acgttcgccg cgcggtcggt tcgcgcctcg
     2401 acgacgtaca agcctctcgc cgagaccagc tccggaagcg ccgaggccgt ctccagccgg
     2461 gagtagccgc agccgtagag cgccgcgacc tgcgcgaagt cgatcccgtg cggcgtcgcg
     2521 aacgcgcgct cgaagtcggg gaccgcgccg gcctgcggca ggaacgagaa gatcccgccc
     2581 ccgtcgttgt tcaggacgac gaagaccgcg tcgaccgcct cgttccgcgc gagcaggagg
     2641 ccgttctggt cgtgcagcat cgacaggtcg cccgcgagcg cgaggacggg accctcggac
     2701 gcgagcgcta cgccgagcgt cgtcgacacg aacccgtcga tgccgttggc tccgcggtta
     2761 cccgtgacac gaaggccgtc gcgcggcgcc atgaaccagt cgaggtcgcg cacgggcatg
     2821 ctcgcggcca ccaccagcgt cgaaccgtcc ggcgcttgcg cagcgaggtc tcgcgccacg
     2881 cgcggctccg aggcctcgtc cgtcgcgtcg aggaccgcgt cgatcgcagc ccgcgcggcc
     2941 cggtccgccg ccgtccactc gtccagccag ccggagcgcc gcggcgccgt gggcgcggct
     3001 gctgcgcaga ggagccccgg ctcggcgacg acgatgcggt ccgcgccgcg ttcggggtcg
     3061 agccaccgcc cgtgcgcgtc gaccagcgcc tgcggaccgt cgcggaagaa cccgttgacg
     3121 acgcttgaga tgccgatctt cccgatgcgg aggacgaagt cggggcgatg gtcctcggcc
     3181 cacccggcgc gcagcaaggc ctcgtacgtc gagatcgcgt tcggcccgta gcgcaggccg
     3241 gacgacggct ccgcgagcac cggccatccc gcggcctcgg ccagcgcgtg gaccccggcc
     3301 gcctcggcgt cgcccgcgcc cgcgaggatc aagccgcgct cgaccgacgc caccttctcc
     3361 gccagccact cgacgtccgc gggggacggc accgcgcggg ctcgcgtcac ggcggtccac
     3421 ggctcgccgc ccgcccggcc ctcgacgtcg gtgacgacct cgtcttcggg cgacgcggtc
     3481 agaggctcgc ggaaacagac gttgacgtga acggggccgg ggtcgcgggc gacggactcc
     3541 gcgaacgcgc ggcacgcgac cgacctccag tagcgggcga gcggcacacg gtcgtcgggc
     3601 acgccggcct cgcagaacca gcgcacggcg tcgccgaaga ccttcacctg gtcgatcgtc
     3661 tggttcgcgc cggtcccgcg cagctccggg ggccggtccg ccgtgacgac gatcatcggg
     3721 accgacgcgt tcgacgcctc cgtgacggcc gggaggaagt tggcgacggc agtgcccgac
     3781 gtcgacacga cgaccgcagg aagcccggtt gcccgggcga tcccgagagc gaggaaggcc
     3841 gaggatcgct cgtcgatcga gacgtgcacg cgcagtctct cgtcggaggt cgccgcgacc
     3901 gccagcggcg tcgagcgcga tcccggcgac aggcacacgt ggcgaacgcc gcaccggtag
     3961 aggtcgtcga gcagacagaa cgccgcggcg tcgcccgcgc tgcgcgcgtt cacgaggccg
     4021 cttcccatcg ctcgatccat tcgtcgccgt gacccgagcc ggtggtctcg aacgcctgca
     4081 acgacgcgtc gtcgaccccg ggacggcaca cccgaagaac gccgtcgacg ggcacgagag
     4141 ggtccgccac gacgtcgccg gcgagcagcg acgccgtccc gagcccgcag gcgtacggga
     4201 gctccggcag cgcggcagcg agagcgaggc ccgcggcgag gccgaccgag gtctcgacgg
     4261 cagacgacac gaccgcaggg agccccgcgg cctcggccac ctcgagacag cgccacaccc
     4321 cgcccagcgg ctggaccttc aggaccacga cgtcggcggc ctcgagccct ttgacgcgca
     4381 gaggatcgtc ggcggtccgc acggactcgt ccgcggcaag cgggacgtcg acgcgcttgc
     4441 ggagcgcggc catctcctcc agcgtcgcga ccggttgctc gacgtattcg aggtcgtagc
     4501 ggccgaggac ctcgatccac ttcgccgcct cgtcgacgtc ccacgcgccg ttgacgtcga
     4561 tgcgaatgcg cccttccggc ccgagcgcgt cgcgcacggc ctcgacgcgc gcctcgtcgt
     4621 ctccctcggc gaccttcacc ttcaccgtcg tgcatccgga cgcgagcgtc atcgcgtgcg
     4681 cctgctcggg tccgacggcg ggaacgatcg cgttcacggg aacgaagtcg cgcacgggtg
     4741 ccggccacgg cttggtagcg gcctccaacg cgcaagcgcg ccatgccctc gtcgcgggtg
     4801 gcccgtactc ggggaacggc gagaactcgc cccagccctg ggggccgcgc acgagcacgc
     4861 ccgatcgctc cgtgacgcgg cggaaccgca cgcgcatcgg tatgcggaac gtccggaggc
     4921 tcaccgcgcg agcgccaccg cgagctgggc ggagaacacc aggtggagta aggccaggga
     4981 gccgagggcg gggacgaggc cggccccctc ggctcgatag acgttcgaaa cgggcacgaa
     5041 cagcacggcg ccacaaccga gcacggcgaa gacgaagctc gggtcgcggc ccgccgtcgc
     5101 gacgagcccc actgcgacgg cacaggccac ggcgaccagg ccggcgagga ggtggcgcgt
     5161 ccggcgcggc ccgagccgta ccgccagcgt ccgctttccc gccgccgcgt cggtcgagat
     5221 gtcgcgcacg ttgttcacga cgaggatcgc cgcggcgacc agcccgaccg ggatcgacgc
     5281 gacgacggcc cggccgagga gctcttccgt gagcacgtac gcggtcccta tcgtcgcgac
     5341 gggcccgaag aacgtgaaca cgaacagctc gccgaggccc gcggatgcat acggccgcgg
     5401 ccccccgctg tacgcgagcg cggcggctat ggacgcgaca ccgacgagca atagctcggg
     5461 gccgacggcg aacgtaagcc ccagtcccgc gaggcccgcc accgcaagcg cccccccgat
     5521 cgccaccttc atctgccccg gctgaaccaa acccgacgcg gtcgcgcggc gggggccgac
     5581 tcgatccgcg gtgtcgacac cgcgcacgcc gtcgaagtag tcgttcgcgt agttcaccgc
     5641 gacctgcagc gacagcgcga cgacgagcgc gccggcgaac ctccaggtat cccagacccg
     5701 agcggctccc gctcccacga ggaccggtgc cacggccgca ggcagagtgc ggggccgcgc
     5761 cgcctcgacc cagacgttca tcccgccctc agggacgttt cgggaacttg tcgaagcgcg
     5821 gcgtgcgctt ctcgaggtac gcgttgcggc cctcctgggc ctcctcgctc atgtagtaga
     5881 ggagcgtcgc gtcaccggcg agctgttgga tccccgcgag cccgtccgtg tccgcgttga
     5941 acgcagcctt cagcagccgc agcgcgagcg ggctcatctg gaggatccgg cgcgcccacg
     6001 cgacggtctc ctcttcgagg cgatcgagcg gcacgacctt gttgacgagc cccatctgga
     6061 gcgcctcctg cgcgtcgtac tgctcgcaca ggtaccagat ctcgcgcgcc ttcttgtggc
     6121 cgaccatccg ggcgaggagc ccggccccgt acccgccgtc gaacgatccg accttcggcc
     6181 cggtctggcc gaagcgcgcg ttctccgcgg cgatcgtgag gtcgcacacg acgtgcagca
     6241 cgtggccgcc gccgatcgcg tatccggcga ccatcgcgat caccggcttg ggcaggcgcc
     6301 ggatctggac ctgcaggtcg aggacgttca gacgcccgac gccgtgctgg tccttgtagc
     6361 cgtcgtcgcc tcggacccgc tgatccccgc cggagcagaa cgccttgtcg ccggccccag
     6421 tgaagatgat cacgccgatc ccggggtcgt cgcgggcgac gttgaaggcg tcctggagct
     6481 cgaagagggt ctggggcctg aacgcgttgc ggacctcggg ccggttgatc gtgatcttgg
     6541 cgatcccctc ggcggtctcg tagacgatgt cgtcgtagct ccgggtgcgc tcccaatcca
     6601 tgttagcgat tgctcctaga gtgatagctc gtacgatcac gcctaccgta gcaggtggcc
     6661 atggatatcc ccgagacaga gctcgtcgcc ctgaggtggc cgccggggcc ggagatggcg
     6721 gcccggctgg agcgcctctg ggccgagggc gcggcggtcc tcccgctgcg atgggacctg
     6781 ccggcccccg agatacgccg gctcctcgat ctcgcccgcc ccgcggccct cgtcgagccc
     6841 gggggcacga cgcggctcga cgaccagctg cccgcgaggg gagccctctg cctggcgacc
     6901 gcggggtcga ccgggccgcc gaagctcgtc gagctgagcc gacaggccct cgaggccgcc
     6961 gcccgcctca cctcggcaca cctcggctcc gagggcgctc gctggctctg ctgcctgccg
     7021 ctcgaccacg tcgccggggt catgacggtc gtccggtcgc gcctccacgg agccgagccc
     7081 gtcgtgctcc cgcgcttcga cgccgcggag gtggcggctg cggacgccga cgcggtgtcg
     7141 ctcgtcccgc tgatgctcga gcgcctgctc gcccacggcg ccgacctgac gaggtggcga
     7201 tggatcctcc taggcggggc ggccgtgagg ccggacctcc tcgacgcggc ccgtgccgcg
     7261 ggcgcgaacg tcgtccggac ctacgggatg accgagacgt gtggcggcgt cgtctacgac
     7321 ggcgagccgc tcgaaggcgt cgaagtgcga gtcgaagagg ggatcgtcgc gctccgcacg
     7381 cccacgctca tggagggata ccgggggcag gctccggttc gcggcggctg gttcgtgacc
     7441 ggcgacgccg gcgagtggga tggccgtcgt ctccgggtcc tgggccgggc cgacgagatg
     7501 atcgtgaccg gcggcgagaa cgtgtctccg gccgaggtcg aggccctgct cgcgtcccat
     7561 ccgaacgtcg ccgaggtcgc ggtcgcgggt gccgccgatc cggaatgggg acagcgggtc
     7621 gtcgcgtacg tcgtcccggc cggggacgcg ccgtcgctcg aagacctgcg tgcgcacgtc
     7681 aaaggtcagg ccgccgcgta caaggcgccg cggcagctcg ttgtcgtcga ctcgctcccg
     7741 cggctcccgt cggggaaggt cgcccgcgcg tccctatcat cgggggcctg ataccaaggg
     7801 ggtcatgagg atcgtgtttc cgaagagggc gctcgccggc gccgccgtgc tgctgctgtt
     7861 gctgggcgcg tgctcggacc cgcaggggac ccagcccggc gaggccgccg cgaccgccga
     7921 cgccgaggac gggtccggct ccggcaccgg cggcgacgac gaagacacca cggcacaagg
     7981 gggctcgaag aacggctccg gcaagggcgg cgatgccaag ggcgggtcga agccggacgg
     8041 cggcgggaac gacggcggct cgggctcagg cggcggcccc cgcggttccg gtggcggcgg
     8101 caaccccggc ttcgccggtg gcgggggagg gtccggcggc tacttcgccg caggcgagta
     8161 cgtctacgac cagcagggct tcgaacggtt ctgccagggc ccccgctgca gcaaggagaa
     8221 gctccccgac accgcgacga tcactgcgag ctacgagagc gactcggtca tcgtcaccga
     8281 ggcgcggtcg tccgatcagc agacgatcac gacgaccacg cgacacacgc ccggccaagc
     8341 gctcataacg aaggtcgtca tcgacttcgc gtacggaagc ttcaacttct cgcagacgta
     8401 cgagcctcag ccaccgatcg agtcgctgtc gttccccctc gaggccggca agacgtggcg
     8461 aggccgctgg aaggcgcaga cgtccggcga ctaccggatg gaggtcaccg gggtgtccga
     8521 cggcgtctac cggatcgaga gcgtcacgaa cttccgcggt gacttctcgg gccgcgcgca
     8581 ggcgacgatc tgggtcgacg gacgcacgcg cgcgatcgtg cggaccgacg gggacgtcgc
     8641 ggtcgcgagc aggttcggcg agtacacgag cagcttcgag acctcgctcc gttccgcccc
     8701 gcaatgagcg ccgcccccac cgcaccggcc cggccggagc ttcccgactg ggactacgac
     8761 ggcgcccccg ggagccgtga ccgcacccga gcaggcatcg cgatcgtcgc cggcgtcgcg
     8821 ctcgccggca cggtcctcgc gtggctcgtg ctcggcccga tcggaatcgc ggccttcgcc
     8881 gctctgttct cgggcgcgat cgtctggacc gtgtcgaact cggggcgcgg gatgttgcgg
     8941 aggtccggcg cggtgcccgt cgtccccgga tcggcgcccc gactcgagaa catcgtcgaa
     9001 gggctcgcca gcaagacgaa gctgcccgcg ccggagctgt ggctgatccg cgacgacgcc
     9061 ccgaacgcgc tcgtctgctt cacgcggcgt ccggcgatcg cgatgacttc gggactggtc
     9121 gagggctaca cgcgcacgga gctcgaggcc gtcgtcgcgc actgcttcgt ccggctcgcc
     9181 gtgacggcga cccccctggc gcggacgatg ctggcgctgt cgacgatctc ctccgcgttc
     9241 ggcgtcccgg tcggcgaccg cgacgacgtc cgtgcggcgg cggtgacgcg ctacccgccg
     9301 gcgctggcga gcgcggtcac gaaggcgtcc ccgcgcagcg gccggggcgc gtcgttctgg
     9361 ttcgtagccg acgacgggat gcacgtcccc gcggccgaac gatcgaaggc cctgctggac
     9421 ctctgacctt cggaaagaag ctcccggccc ccgtatcctg ggctttcgga ctcgtcgcag
     9481 gaaaggaacc ccctcatgtc cgacaccacc gcacgagaga ccgggggcct gcgcgtcaag
     9541 cgcggcctcg ccgagatgct caagggcggc gtcatcatgg acgtcaccac cgccgagcag
     9601 gcgaagatcg ccgaggaggc cggcgccgtc gcggtcatgg ccctcgagcg cgtccccgcg
     9661 gacatcaggg ccgagggcgg cgtcgttcgc atggccgacc ccgagaaggt ctccgagatc
     9721 atggagtccg tctcgatccc ggtcatggcc aagtgccgga tcggtcactt cgtcgaggcg
     9781 caggtgctgc aggcgctcgg ggtcgactac gtcgacgagt ccgaggtgct gacgcccgcg
     9841 gacgaggcca accacgtcaa caagtgggac ttcgacgtca cgttcgtctg cggcgcgacg
     9901 aacctcggcg aggccctgcg caggatcggc gagggcgcgg ccatgatccg cagcaagggt
     9961 gaggccggca ccggcaacgt cgtcgaggcg gttcggcaca tgcgctcgat caccgggacg
    10021 atccggaagc tcgcgtcgct gggctccgag gagctgatgt cggaagcgaa ggagctgcgc
    10081 gcgccgttcg acctcgtgcg tgaggtggcc gagaccggga agctgcccgt cgtgctgttc
    10141 acggccgggg gcatcgcgac gcccgcggac gcggcgctga tgatgcagct cggtgccgac
    10201 ggcgtgttcg tcgggtccgg gatcttcaag tcgggcgacc ccgcgacccg cgcccgcgcg
    10261 atcgtcgacg cgaccacgca cttccaggac cccggaatcg tcgcgaaggt ctcgcacggc
    10321 ctcggcgagg cgatggtcgg gatcaacatc gccgacatcc ccgacgccga ccggctggcg
    10381 aagcgaggct ggtaggccgg gtgaaggtcg gcgtcctcgg gctccagggc gacttccgcg
    10441 agcacgcgcg cgccctcgag gactccggcg cgacggccgt cgtcgtcaag acaccagaac
    10501 agctcgcggg cgtcgacgcg ctcgtgatgc ccgggggcga gtcgacgacg atcggcaagc
    10561 tcctcgaccg cttcgagctg ctcgagcctc tgcgggaacg cgccgcctcc ggcatgccgc
    10621 tgtacgggac gtgcgcgggg ctgatcctga tggcgaatcg gatcgagggc gagcaggacg
    10681 caccgcaccg cctcgggctg atggacgtcg ccgtgcgtcg caacgcctac gggcgccagg
    10741 tggactcgtt cgagaccgac ctcgacgtcg ccgggctgga cgagccgtac cgggcggtct
    10801 tcatccgcgc ccccgtggtc gagagggcgg gcgccggcgt cgaggtgctg gcctcggtcg
    10861 acggcaagcc cgtgctcgtg cgccagggga cgatgctcgg ctcgacgttc caccccgagt
    10921 tgaccggcga caatagggtc cactcgatgc tcgtagggat ggtgaggcag tagatgtccg
    10981 gacactccaa gtggtccacg atcaagcgca agaagggcgc ggccgacgcg cagcgctcga
    11041 aggtgtgggc gaagctcctg cgcttcgtgg aggtggcggc gcgcgagggc ggcggcaaca
    11101 tcgacgcgaa cccgacgctt gcgaccgcgg tccagaaggc gaagtccagc tcggtgccga
    11161 acgacaacat cacgcgtgcg atcaagcgcg gcaccggtga gcccgccggc gaggcctacg
    11221 aagaggtcag ctacgagggc tacggccccg gcggcgtcgc cgtgctcgtg cggtgcctca
    11281 cgagcaatcg caaccgcgcc gcgcaggacg tgcgcagcat gttcaacggc aacggcggca
    11341 agatggccga gccggggtcg gtcgcgtgga tgttcgagac caagggcgtg atcgtcgtca
    11401 cgtaggactc gatgagcgag gaggacgcgt ttctggtcgc ggtcgacgcc ggcgcggaag
    11461 acgtgcgcgc gtcggacgag agcatcgagg tcgtgactcc gccggacatg gtgaaggccg
    11521 tgcaggacgc gctcgcgggg gcgggggccg aggtcgagtc ggccgacctc accgaggtgc
    11581 cgaagtccac gatcgcggtc gagggcagcg acgcgaagaa ggtgctgaac ctcgtggacg
    11641 cgctggaaga gctcgacgac gtgcaggacg tgtacgcgaa cttcgacatc tccgacgatg
    11701 tcctcgcgac gctcgcttcc tgacgacccg cgcctgcggg aggtagccga gcagctcgcg
    11761 gcgacgcgct gggccgcgtc gatcgtcgac gacgcctaca agctcgtctg ggtttccgac
    11821 gagatggcga acctcctcgg cgagcaggac gagggcaagc tcggctacgg caagcacctg
    11881 ctcgaagccc agatcggcga gacgtggtgc aaggtaatta cggacgccag ccacgtctcg
    11941 gccttcgagg cggagctgcc gatcatgatc gaagggactc ccggcgggaa ggaagcgctg
    12001 ctcgagacct tgggcggcgc gagcgtcggc ctctccgacg agctcgtgac cgaggacatg
    12061 gtgcggcggt tcgtcgagat ggagccgcag cgccccccgt cggtgtgggt caaccacctc
    12121 gagttcatcc agggcgagct gccgccggtg aaggtgaccg aggtccagat caagctctac
    12181 gagaaggact cgggtgagtt catcgggcgt gcgttcctct acggtcccgc gctggcggcg
    12241 agcgtgctgt cgctcgtcgc gcgcggcgag gaggacatgt tccagcggat ggcgcgcctc
    12301 gtcgatccgg ggcgtcggca ggccgccgtc ttgttcgccg acctcgaagc atcgtcggtg
    12361 ctgtcgcgtc gcctccccag ctcggcgtac ttcaagctcg tgcgcgcgat cacgactgcg
    12421 atggacgacc aggtcgtgaa gcacaccggg atcgtgggca agcacgcggg cgacggcgtc
    12481 accgcgttct tcttggccga ggacctaggg tctccgtcgc gcgcggcgag ggccgcgatc
    12541 gaggcaggtc ggggaatgat cgagaccgcg aggggggccg ccggagagat ccgcgacgag
    12601 accggcctgc tcgagctcga cgactgcgtg atcaacgtcg ggatccactg gggcggtgcg
    12661 ctctacatgg ggcagctggt cacgggcggg cgtctcgagg cgaccgccct gggcgacgag
    12721 gtcaacgagt gcgctcggat ccaggagacg tcccgcgccg ggaagatcct ggcgtcgaag
    12781 gccctggtcg agcgactgac cgacgacgac gcgcgtgccc tgggcctgga ccccgacagc
    12841 gtgctgtact gcacgatcgg ggacatagag ggagcctcgc cgaaggccaa gcgcgacgcg
    12901 gggggcatcc ccgtaacgcc cctctagtcg acgcaggtct tcttcagccc gtcgcgggtc
    12961 gtgtcgtcga agcggtggtc gatcgtcacg ctgccgtggt tcagcgtcac ctcggccccc
    13021 gtgggggagc gccagcgcac tgcgtagcga cggcattcga tgccgcctcc ccggcgcgtg
    13081 tctacgaagt cgtacgagaa ggactccgcg tcacccgccg gcgtgaagtg ggccactcca
    13141 ggctcgagct ggcggcgggg caggcgcagg atctcgatct cgacggggcc gccgctgaag
    13201 tcgccgctga acctgatcgt cgcggcgccc cgcgcctgca cgaacaactt catcagcggg
    13261 atcttgcgga agcgcgtctc cgccgtcgtc acggcctcgg tgcggtacac cgtcgtccgg
    13321 cgggtcggcg cgtagctcgt caccccggca tcgggttggc tgtccatcag ggcgtacgcc
    13381 ggaacggcgg ccagcgcggc tgcggccacg gtggcgaccg ccatggaacg gcggctcaca
    13441 cgcatcagat cgggagaagc cccgcgcgcc acttccaggt cgcccaacgc tcgccgcagc
    13501 gcgggatccg cttccactcg gccttgacct ccaggtacag gtccggccac ggcggcggcg
    13561 acaggaccgc ctcccacatc gtctcgccct cgacctcgac gcggcgcagc tcgctcggga
    13621 cctctagctg cccgtagatc ggcgtcccgg tcgtcgggtc cccgaacagg aacgccctgg
    13681 cctccacgcg gcgaggccgc tcggcgctcg cgatcctgac cgcgacctcg gtgcccggca
    13741 cccaccggac cggtggcgac gggaagccgt actcgccctc ggcgaaggcg atcacgcact
    13801 cccagctcga gcgggtcgcc cacctcgccc cggcgaagct gcccggctcg gacgcgtccg
    13861 ccgtgacgag cgacgcgtgc ggccagggcc ggtggtccgc gaccgccgtg gccggaaggc
    13921 agagcgccgt taccagcgca gccgcgaaga gcttcgtccg catgccctcc aacttcggct
    13981 gcgctcggcg attctcctgc gtggaggcat gttcaaatcg acccatcgac gagcgcctgg
    14041 gcgcatgggc ccaccacatc gagaagctcc agtggtccgc gatcgtcctg gactcggagt
    14101 ggcgcttcgt ctgggcgtcg tcggagatgg ccgagttcat cggcgaggac gacggcgaca
    14161 agctcggcta cggcaagcac atcgccgagg cgttcctgtc cgacgtgtgg ctccgcacgg
    14221 tccacccgga cagccaggtc gagctcttca accacctcgc gccgtacctg ctgcacgact
    14281 tcgcacggcg cggccgcgac ccgaaggacg tccttcccga gagcttcctg ccgttgctcg
    14341 aggggatcga gccgacggac ccgccggcga tggactcgat ctcgttcgcg tacgtgaacc
    14401 cgagctcgga cgaggatctc ccggactacc gcgtgaacgg cctgttcctg aggctcaacg
    14461 acgacgccgg cgagctgatc ggctggctct gcgtgttctt catggacatc cggccgaacc
    14521 tgctggtgct gctcgcccgc ggcgacgagc agatgtacga gcggatggcg aagctcgtcg
    14581 accccggccc ccggcaggcc gcgatcatgt tctgcgacct gcacggctcc gggacgctgt
    14641 cgcgccggct gccgagcgcg gcgtacttca agctcgtgag acgactgtgg cgggggatcg
    14701 acgccgcggt cgccgagcat tcggggatcg tcggcaagca tgcaggcgac ggcgcgtcgg
    14761 cgttcttcct cgtcgacgac ctcggatcgc cgtcggcggc agcggcggcc gctattcggg
    14821 cggcggcccg cgtccacgag atctcgcacg gcgtcttcga ggacgcgctc gagtccgact
    14881 gcctgatgaa ggtcggcctc cactggggcg gcagcctcta catgggccag ctcgtgcccg
    14941 gcgggcgcct cgacgtgacg gccctggggg acgaggtcaa cgaggccgcg cgcatccagg
    15001 agaccgcggg ggccggggag acgctggcgt cgaagcagct ggtcgagcag ctgacgaccg
    15061 acgaagccgc ggccctcgga gtcgacgtcg agaagctctc ctaccgcccg ctggcggaga
    15121 tcgagggcgt tccggagaag gccgcccgcg acgccggggg cctggcggtg acgaaccagc
    15181 tgggccacta ggaaaaccgc cggtaccggc agctcccggg ataacctccc gaacaagcgt
    15241 tcgccttcgg aaggggagac agcacgtgcg cgtcctcggc atcgatcccg gcttcgccac
    15301 caccggctac ggcgtcgtcg agcgcatcgc cggcgcgctc cggcccgtca cgatcggcgt
    15361 cgtccggacg cccccggccc tcccgcaggc ggaccggctc gcccacctcg ccggggccct
    15421 ccgaggcgtg atccatgcga cgcagccgga cgtcgtcgcc atcgaacggc tcttcttcaa
    15481 ctcgaacgtc cgcacggcga tgagcgtggg ccaggcctcc ggcgtcgccc tgctcgccgc
    15541 ggccgaggcc gggctcgagg tcgtcgacta cacgccgtcc gaggtcaagc tctcggtcgc
    15601 aggcgtcggc aacgcggcga agccccaggt gcaggcgatg gtcgcggcgc tcctcaagct
    15661 ccaggccgtc cccaagcccg ccgacgcggc cgacgcgtgc gcgctcgcga tctgtcacct
    15721 gaaccgcagc ggcctgagcc gcgccgttgc agctgcacgg aaacgagccg gatgatcggc
    15781 ttcctgcgcg gggccgtcgc ggcccgcacg accgacggct gcttcctcga ggtcgccggc
    15841 gtcggctacc ggctcgcgtg ctcggcgacc acgctcgcgg cgctcccgcc cgagggcggc
    15901 gaggtccggc tctggaccca cctccacgtc cgcgaggacg cgctgtcgct gttcgggttc
    15961 gcgaccgagt ccgagcagcg catcttcgag gccctgctcg gcgtcaacgg cgtcggcccg
    16021 aaggtcgcgc tccaggtgtg ctccgccttc acgcccgacg cgctgcgcaa ggccctcgtg
    16081 accgacgacg tcgctgcgat ctcgtcggtg ccgggcatcg gcaagaagac cgcgcagcgg
    16141 atgatcctcg acctcaagga gaagctggcg ctgccggacc tcgaggtcgt cggcgtccgc
    16201 cccgacgcgc tgtcgaaggc gcgctccgcg ctcgagaacc tcggctactc ggcgggcgag
    16261 gtccgcgtcg ctctcagcga ggccgtcccg gccgaggagg ccagcgtcga ggacatcgtg
    16321 aagtccgctc tgaaggtgct cgcctgatga ccgacgaggt cctggccaag gtcgcaaacc
    16381 cggaggacgt cgaggtcgac cgttcgctcc gaccgagaac actcgtcgag ttcgtcggcc
    16441 agcccgagct caaggagcac ctcgcgatcg tgcttcaggc cgcgcgcgag cgcaacgagc
    16501 ccgccgggca cctgcttctc tccggccccc ccggcctcgg caagacctcg ctcgcccacg
    16561 tcgtcgcgaa cgagatggac gcgtccatac ggccgacctc cggcccggcg ctcgagcgcg
    16621 ccggcgacct cgccgcgatc ctcacgaacc tcgaagacgc cgacgtcttg ttcatcgacg
    16681 agatccaccg gctcccgcgg gcggtcgagg aggtcctcta cccggcgatg gaggacttcc
    16741 agctcgacct cgtgatcggg aaggggccga gcgcgcacac gatccggctc gacctgccgc
    16801 gcttcacgct catcggcgcg acgacgaggg ccgggctcat caccggcccg ctacgcagcc
    16861 gtttcgagct cgccgagcgc ctcgactact acccgccgga ggacctcgag cggatcgtcc
    16921 gccggtcggc cgcgatcctc gacgtctccc tcgacgagga gggcgcgctc gagatcgccc
    16981 gccgctcccg ggggactccc cgcatcgcga accggctgct gcgcagggtc cgcgacttcg
    17041 cccaggtgcg cgcgaacggt tccatcgacg gcgacgtggc ccgcgacgcg ctcgcggtct
    17101 tcaaggtcga cgacaagggc ctcgacaaga ccgacggggc gatcctcgaa gccctcgtgc
    17161 gccgattcgg cggtggcccg gtcgggctcg ggaccctcgc agtgtccgtg ggcgaggagc
    17221 ccgacacgat cgaagacgtc tacgagccct tcctgatgca gctcggcctg ctccggcgca
    17281 cgccgagggg ccgggtggcg acggaggccg ccttcgcgca cgtcggggcg ccgctgccca
    17341 agggggccgc ggcccgcctg ttcgagtcct aggaccggtc ccccggtagg atgcccccgg
    17401 aaacccagct t
//
LOCUS       DASQDO010000416         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_43881, whole genome shotgun sequence.
ACCESSION   DASQDO010000416 DASQDO010000000
VERSION     DASQDO010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43881"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VEV43_11660"
     CDS             complement(<1..918)
                     /locus_tag="VEV43_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYP24221.1"
                     /translation="MSEQTERLDALAAKVRGGGAPKYHEKLAQQGKLFVRDRLTLLLD
                     SADGFAEDGLLVNALAGDLPADGVVTGVGTIDGRPVAVMANDPTVKAGSWGARTVEKI
                     IRIIELARKNEHPMIYLVDSAGARITDQIDLFPGRRGAGKIFYNQVQASGSIPQVCCL
                     FGPSAAGGAYIPAFCDVVLMVDGNASMYLGSPRMAEEVIGEKVTLEEMGGARMHSAVS
                     GVGDMVVADDKAAIEAAKGYLSYFGQSWRDAAPRVQARPPAFRGSLEGIVPEDESQAF
                     DMYAFVAGIVDEESFFEIKPDFARELITGL"
     gene            1144..>4088
                     /locus_tag="VEV43_11665"
     CDS             1144..>4088
                     /locus_tag="VEV43_11665"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYP24222.1"
                     /translation="MNVRLFKRAAIAVATALVAGLVTGAPVVAQDELSESPQDSREEL
                     VGRRTQSSKTFRNPDGTVTTRLFSGPVHYRGRDGRWKEIDSSLVAADEPGYALRNAAN
                     DFDVLFKEELRDGYVRVSTGGMDFDFSLTGAARAAARRAHSNGLRFGDAYPNVDLDYE
                     VFDSGVKETLVLDNANVPNRYRFVMEASGEGDAYVGRTPEGAWAVFTSRSADPAIVFA
                     APTVMEGAASPVEDPEPSPSPDPSPSPSAEPSPSAPPESAETDADAEATQQPVESGPS
                     PTPPAPSPAETEATTSPDVPAETSDPGRPSKPESSPSNVDARQMQRAAALDVRELGRN
                     RFEVVLELDPRWLHDPERQFPVLLDPTTLTVQPTADDLSYAWACNSCVGHPDYRIYVG
                     TGYQDVWRAALKFDTSALPVGTVSAAKLSLYHEGSCVSNAPWCTNAAHSIEAYRMTKA
                     WGPSTTVGTLEYQWPPIATYNLAAGATNLWMNWDATGTVQAWVDDPTTNRGILLKRTV
                     ETLGSNGAIAPLGHRHWDTYRRPKLDVTYTDLPVTLEVPETWHSNGAELSWTKFSSSA
                     GGTFTGYEIHRSDNRDFRPSADTLLTTISDVNTTRFVDTTAGSSRSYTYRIRTGSISS
                     NGQTGSTPADGMATKVLNGRWYGGDDTYLMYDKNYEVCDSYGEHNRMWVGSTSWSKMR
                     SLVRFPVSDIPPGATVESAKLLLHQEGWLPAAGLKVNVHRATKDWSAGHAWAGCTLPG
                     ANWYRYDGVGRWGADGGDFDPAVEASVTTDGSTTPKWDNWDVTPLVQRWVRGEVPNFG
                     MLLKAETEPLAGDLRITYDAADNYFGEHFNPRLEILYRDGSRSVGPSVAIASPGSGET
                     VRGPTPVKVTATDDRKVTKVELLVDGTLSSTDTTAPYEFTWSSNSVANGAHNLTAKAY
                     DDVGNVTTSAAVPVTVANSAVPAAQITQPAGGATVAGSVTVSATASDDVGVSKVEFLF
                     DGYRFGEDLV"
BASE COUNT          704 a         1413 c         1349 g          622 t
ORIGIN      
        1 gagcccggtg atcagctcgc gcgcgaagtc cggcttgatc tcgaagaacg actcctcgtc
       61 gacgatcccc gcgacgaacg cgtacatgtc gaaggcctgg ctctcgtcct cgggaacgat
      121 cccctcgagc gagccgcgga aggcaggggg ccgggcttgg acgcgggggg cggcgtcgcg
      181 ccacgactgg ccgaagtacg agaggtaccc cttcgccgcc tcgatcgccg ccttgtcgtc
      241 cgcgacgacc atgtccccga caccagagac ggcgctgtgc atgcgggccc cacccatctc
      301 ctcgagcgtg accttctcgc cgatcacttc ctccgccatg cgcggcgaac ccaggtacat
      361 cgacgcgttg ccgtcgacca tgagcacgac gtcgcagaac gcagggatgt acgcgccacc
      421 cgccgccgac gggccgaaca ggcagcacac ctgcgggatc gaaccggagg cctggacctg
      481 gttgtagaag atcttgcctg cgccacggcg gccggggaag aggtcgatct ggtcggtgat
      541 gcgcgcgccg gccgagtcga cgagatagat catcgggtgc tcgttcttac gcgccagctc
      601 gatgatccgg atgatcttct cgaccgtccg ggccccccac gacccggctt tgaccgtggg
      661 gtcgttggcc atgacggcga cgggccgccc atcgatcgtg ccgacgccgg tgacgacgcc
      721 gtccgcgggc aggtcgccgg cgagggcgtt gacgaggagc ccgtcctcgg cgaacccgtc
      781 ggcggagtcg aggagcaggg tcaagcgatc gcgcacgaac aacttcccct gctgggcgag
      841 cttctcgtga tacttgggcg cgccgccgcc cctgaccttc gccgcgaggg cgtcgagccg
      901 ctcagtctgt tcgctcacgc ccgcatccta attcgccgtc acgcccctac tcatggcaag
      961 agcggaggtc cgaactaagc gccggtcctt tgtgactagc aagcaaattg gtcatcgcgc
     1021 cagcgaggat tttcgtcgat ccccctggcg gcccgtccct atcggtcttt agctttcttc
     1081 cttcagcgac ataccaagct ttctccagga cgacacctcg ttcgaggtgg gagggggatt
     1141 ccgttgaacg tacgtctgtt caagcgcgcc gcgatcgcgg tggccacggc gctcgtagcc
     1201 gggctcgtga cgggagcccc cgtcgtcgcc caagacgagc tgagcgagtc gcctcaggac
     1261 tcgcgtgaag agctcgtcgg gcgacggact caatcctcga agactttcag gaaccccgac
     1321 ggcacggtga ccacccggtt gttctccggc ccggtccact accggggacg cgacggacga
     1381 tggaaggaga tcgactcctc gctcgtggcg gcggacgagc cggggtacgc gctgcgcaac
     1441 gccgccaacg acttcgacgt gctcttcaag gaggagctgc gagacggcta cgtccgggtc
     1501 agcactgggg gcatggactt cgacttctcg ctcacggggg ccgcccgggc tgcggcccgg
     1561 cgagcccact cgaacggcct tcgcttcggt gacgcctatc ccaacgtcga cctggactac
     1621 gaggtcttcg actccggggt caaggagacg ctggttctcg acaacgccaa cgtccctaac
     1681 cggtacaggt tcgtgatgga ggcgtcgggt gaaggggacg cctacgtcgg ccggaccccg
     1741 gaaggcgcct gggccgtctt cacgagcagg tccgccgacc ccgcgatcgt gttcgcggcc
     1801 cccacggtga tggagggcgc agcctctccc gtcgaagatc cggaaccgtc gccgtcaccc
     1861 gacccgtctc cgtcgccttc ggcggagccg tctccttctg ctccaccaga gtcggccgag
     1921 acggacgccg acgctgaggc gactcagcag cccgtcgagt ccgggccctc tcccacgcct
     1981 cccgcaccgt ctccggcgga gaccgaggcc accacgtcac ccgacgtgcc ggccgagacc
     2041 tccgacccgg gtaggccttc caagcccgag agctcaccgt cgaacgtcga cgcccgtcag
     2101 atgcagcgcg ccgcggccct cgacgtccgc gagctcggcc ggaaccggtt cgaggtcgtc
     2161 ctggagctgg accctcgatg gctccacgat ccggagcgcc agttccccgt gctgctcgac
     2221 ccgaccacgc tcacggtcca gccgacggca gacgatctca gctacgcgtg ggcttgcaac
     2281 tcgtgcgtcg gacaccccga ctaccggatc tacgtgggca ccggctacca ggacgtatgg
     2341 cgcgcggcgc tcaagttcga cacgagcgcg ctgcccgtag gaacggtgtc agccgcgaag
     2401 ctgagcctgt atcacgaagg cagctgcgtc tcgaacgcgc cgtggtgcac gaacgcagcc
     2461 cacagcatcg aggcctaccg catgacgaaa gcgtgggggc cgagcacgac ggtcggcacg
     2521 ctcgagtacc agtggccgcc catagcgacg tacaacctcg cggcgggggc gacgaatctg
     2581 tggatgaact gggacgcgac cgggaccgta caggcctggg tggacgaccc gaccacgaac
     2641 cgcggcatcc tcctcaagcg cacggtcgag acgctcgggt cgaacggtgc gatcgctccg
     2701 ctgggacatc ggcactggga cacctacagg aggccgaagc tcgacgtcac ttacacggac
     2761 ctccccgtca cgctggaggt tccggagacg tggcattcga acggagcgga gctctcctgg
     2821 acgaagttct cttcgtcggc tggggggacg ttcaccggat acgagatcca ccgatcggac
     2881 aaccgcgatt tccggccgtc cgccgacacg ctgctgacga cgatctcgga cgtcaacacc
     2941 acgcgtttcg tcgacacgac tgcggggtcg agccgctcct acacgtaccg catccggacg
     3001 ggatcgatct cctccaacgg acagaccggg agcacgcccg ccgacgggat ggccacgaag
     3061 gtgctcaacg gtcgctggta cgggggcgac gacacctacc tcatgtacga caagaactac
     3121 gaggtgtgcg actcgtacgg agagcacaac aggatgtggg tgggttccac gagctggtcc
     3181 aagatgcggt cgctcgtccg gttccccgtc tccgacatcc cccccggcgc aaccgtcgaa
     3241 agcgccaagc tcctgttgca tcaagagggc tggctccccg ccgcgggcct gaaggtcaac
     3301 gtccaccgcg cgacgaagga ctggtccgcc ggtcacgcgt gggcggggtg caccttgccg
     3361 ggcgcgaatt ggtacaggta cgacggtgtg gggcgatggg gtgccgacgg cggggacttc
     3421 gaccccgcgg tcgaggcctc ggtgacgacc gacgggtcga cgacgccgaa gtgggacaac
     3481 tgggacgtga cgccgctcgt gcaacgctgg gtgcgcggtg aggtcccgaa cttcgggatg
     3541 ctgctcaagg ccgagaccga gccgcttgcg ggggatctgc ggatcaccta cgacgcggcc
     3601 gacaactact tcggcgagca cttcaacccc cggctcgaga tcctctaccg cgacggcagc
     3661 cggtcggtcg gcccttcggt cgccatagct tcgccggggt cgggcgagac cgtccggggg
     3721 ccgacgccgg tcaaggtcac cgcgaccgac gaccggaagg tgacaaaggt cgagctgctg
     3781 gtcgacggga cactgtcgtc gaccgacacg accgcgccat acgagttcac gtggagctcg
     3841 aactcggtcg cgaacggcgc acacaacctc actgccaagg cttacgacga cgtcggcaac
     3901 gtcaccacgt ccgcggcggt ccccgtcacc gtcgcgaact cggcagtccc ggcggcacag
     3961 atcacgcagc cggccggcgg agccaccgtc gcgggatcgg taaccgtgtc ggcgaccgcc
     4021 tccgacgacg tcggagtgag caaggtcgag ttcctgttcg acggttaccg gttcggcgaa
     4081 gacctcgt
//
LOCUS       DASQDO010000417         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_412076, whole genome shotgun sequence.
ACCESSION   DASQDO010000417 DASQDO010000000
VERSION     DASQDO010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412076"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VEV43_11670"
     CDS             <1..1111
                     /locus_tag="VEV43_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYP24223.1"
                     /translation="SNPAEAIAAGFVAGAVMESRGAGAAAAAAAAPLVSGADPVSLGI
                     GAAAALVSRRVWPVPPSDGAEARKVWPRGRAEPRSGGEGLFVAVNTASGNGDSPADAL
                     RRELPRAEVEEVDLVDGDELRKALDEGAEHATALGVSGGDGSINTAAQVALENDSALA
                     VFPSGTFNHLAGALAIEGADDTFAAIEDGEAVAIDAATIDGHVFLNTASFGAYAELVD
                     MRERLEHRLGKWPAVLVALVRVLRHSRPVAVEIDGRPRKVWMAFIGNCRYSPSGFAPS
                     WRRRLDDEQIDFRCVDGSTPFARTRLVLAVLTGRLGRCKVYEQRVVKELRIQSLEGPL
                     RLARDGETFMSSGDEVVVRKLPQRLAVYCPHEQVG"
     gene            1108..1758
                     /locus_tag="VEV43_11675"
     CDS             1108..1758
                     /locus_tag="VEV43_11675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYP24224.1"
                     /translation="MSAVLSLQALRAPAAKRVLLVLAVIRGVLAVAAIPLAPFLFEDH
                     FLVLVLLRPAKEMFLAGGFLARGGEVDLLLLVLAAIPLSIFGVWHFYLLGRAYQGEIE
                     SGKLSGLAARVLTPERVEKLERSLERKGPKLIFLGRLAVLSSAAVAAAAGAAKMEPRK
                     FFPWDLGGGLLSIAYTVAAGWFLGAAYEEAGPWITAAGVAALLGFAFVLGRTLQRA"
     gene            1758..>2317
                     /locus_tag="VEV43_11680"
     CDS             1758..>2317
                     /locus_tag="VEV43_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYP24225.1"
                     /translation="MSRGLPGWAQRRFDPSGRYGLRLTLFALAATLVAVPFSFLLLQV
                     LDSGPLTEVDQGAAEAIHEWIRDSRFLVALAFFFSFLGVPPWFYVTVGASAYWFYRRG
                     YWRVALFMVTTPLIGGVISTTVKVLVDRPRPELDDPITHAFGKSFPSGHAMTSTVAYG
                     TLLLAFMPMLPPRWRPRAIAGYVALVL"
BASE COUNT          275 a          835 c          819 g          388 t
ORIGIN      
        1 ctcgaaccct gcagaggcga tcgccgccgg cttcgtcgcc ggcgcggtga tggaatcccg
       61 gggagccggt gccgccgctg ccgccgccgc ggccccactc gtctccggcg cggacccggt
      121 ctcactcggc atcggcgccg ccgcggccct cgtctctcgg cgcgtctggc cagtcccgcc
      181 ctccgacggg gccgaggctc gcaaggtctg gccccgcggg cgggccgagc cgcgctccgg
      241 tggcgagggc ctgttcgtcg cggtcaacac cgcgtcgggc aacggcgact caccggccga
      301 cgcgctgcgg cgcgagctgc ctcgggcgga ggtcgaagag gtcgacctcg tcgacggcga
      361 cgagctgagg aaggcgctgg acgagggggc cgagcacgcg accgcgctcg gcgtctcggg
      421 cggcgacggc tcgatcaaca cggccgcgca agtcgcgctc gagaacgact cggccctcgc
      481 tgtcttcccg tcgggcacgt tcaaccacct cgccggtgcg ctcgcgatcg aaggagcgga
      541 cgacaccttc gccgctatcg aggacggcga ggccgtcgcg atcgacgccg ccacgatcga
      601 cgggcacgtt ttcctgaaca ccgcgagctt cggcgcgtac gcggagctcg tcgacatgcg
      661 tgaacgactc gagcacaggc tcggcaagtg gccggcggtc ctcgtcgcgc tcgtccgcgt
      721 cctgcgccac tcgcggccgg tcgcggtcga gatcgacggc cgtccccgga aggtgtggat
      781 ggctttcatc ggcaactgcc ggtactcgcc gagcggcttc gccccgtcgt ggcgccggcg
      841 cctcgacgac gagcagatcg atttccgctg cgtcgacggg tccacgccgt tcgcgcggac
      901 gcgtctagtc ctcgcggtgc tcaccggacg cctcggccgg tgcaaggtct acgagcaacg
      961 ggtggtgaag gagctgcgga tccagtcgct cgaggggccg ttgcgcctcg cccgcgacgg
     1021 agagacgttc atgtcgtccg gcgacgaggt cgtggtccgg aagctcccgc agcggctcgc
     1081 cgtctactgc ccccacgagc aggtcggatg agcgcggtcc tgagcttgca ggccctccgg
     1141 gcccccgcgg cgaaacgggt cctcctcgtc ctcgcagtca tccgcggggt cctcgccgtc
     1201 gcggcgatcc cgctcgcgcc cttcttgttc gaggaccact tcctagtcct cgtcctcctg
     1261 cgcccggcga aggagatgtt cctcgcgggc ggcttcctcg cgcgcggggg cgaggtggat
     1321 ctcttgctgc tcgtcctcgc ggcgatcccg ctgagcatct tcggtgtgtg gcacttctac
     1381 ctgctgggcc gcgcctacca gggcgagatc gagagcggga agctctcggg cctcgcggcg
     1441 cgcgtgctca cgccggaacg cgtggagaag ctcgaacgtt cgctcgagcg caagggaccg
     1501 aagctgatct tcctcggccg cctcgcggtc ctgagctcgg ccgccgtcgc ggctgcggcc
     1561 ggcgctgcga agatggagcc gcgcaagttc ttcccgtggg acctcggggg tggcctgttg
     1621 tccatcgctt acacggtcgc cgcagggtgg ttcctcggcg ccgcgtacga ggaggcgggg
     1681 ccgtggatca ccgccgccgg cgtggccgcg ctcctcggct tcgcgttcgt cctcggccgg
     1741 accttgcaga gggcgtagtg agtcgcggcc tgcccggctg ggcacagcga cgattcgacc
     1801 cctcgggccg ctacggcctg cggctgacgc tgttcgctct cgccgcgacg ctggtcgccg
     1861 tcccgttctc gttcctgctg ctgcaagtgc tcgactcggg accgttgacc gaggtcgacc
     1921 aaggagccgc cgaagcgatc cacgagtgga tacgcgactc gcgttttctc gtcgcgctcg
     1981 cgttcttctt cagcttcctg ggcgtcccgc cgtggttcta cgtgaccgtc ggcgcgtccg
     2041 cgtactggtt ctaccggcgc ggctactggc gggtcgcgct gttcatggtg accacgccgt
     2101 tgatcggcgg ggtgatcagc acgaccgtga aggtgctcgt cgaccggccg cggccggagc
     2161 tcgacgatcc gatcacgcac gcgttcggca agagcttccc gtcgggtcac gcgatgacgt
     2221 cgacggtcgc gtacggcacg ctgctgctcg cgttcatgcc gatgctcccg ccgcgctggc
     2281 gcccgcgcgc gatcgccggc tacgtcgcgc tggtcct
//
LOCUS       DASQDO010000418         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_472873, whole genome shotgun sequence.
ACCESSION   DASQDO010000418 DASQDO010000000
VERSION     DASQDO010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472873"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="trpS"
                     /locus_tag="VEV43_11685"
     CDS             <1..648
                     /gene="trpS"
                     /locus_tag="VEV43_11685"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYP24226.1"
                     /translation="RQRELVSAGLFLYPVLQAADVLAYRATEVPVGEDQRQHIELMRD
                     VGARFNAQLGETFRLPEPLIPEKGARIMALDDPTQKMSKSAGRPASYIGLVEPENVVA
                     KKVKSAVTDSGREVRAADDKPALTNLLTIFSLVEGTPVPELEERFASAGYGDFKKALA
                     EVVVERLRPIRERYEELVGDPAEMEAILRRGAERAAAEAELTMKVVRERTGLLPA"
     gene            838..1632
                     /locus_tag="VEV43_11690"
     CDS             838..1632
                     /locus_tag="VEV43_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HYP24227.1"
                     /translation="MAYEVKLEVFEGPIDLLLHLITRQRVDIYDVSLARITEEYLAAI
                     SELDGTDLETATGFLVVAATLLELKSARLLPGRTGDEEETHLLEERDLLLARLVECST
                     FREAGAWLARGLEAGSAAHGRAAGLEERFVDLAPKIVLKTTPERLGALAAAALAPKPE
                     PEVDMSHVQPIRATVRDAILEVASYLRSLGAPAGFRELCSGADERIDVVVRFLALLEL
                     FKAGAVDLVQVDRFGDIRASWTGAAEADEVLADADEYVIDLREAGR"
     gene            1629..2216
                     /gene="scpB"
                     /locus_tag="VEV43_11695"
     CDS             1629..2216
                     /gene="scpB"
                     /locus_tag="VEV43_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYP24228.1"
                     /translation="MNDAPESAPATRKIVEAILLLADEPVPASQMGEVLEVPRADVEA
                     VLRELADEYARDDRGFVLRESAPGWRLYTDPECAPWLERFVTGHRHARLSGASLEVLA
                     IVAYKGPISRSEISEIRGVDSDGVVKTLLQRGLIEASGPGGPGGPGAANLFEVSDEFL
                     ERMGLRSVDDLPPLTDFMPDAEAVEDMEAKLSPNA"
     gene            2219..>2754
                     /locus_tag="VEV43_11700"
     CDS             2219..>2754
                     /locus_tag="VEV43_11700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYP24229.1"
                     /translation="MGDEGERLQKVLAHAGLGSRRAMEDLIAAGRVKVNGRRAQLGAR
                     VDPDKDEVEVDGSRVPLRQELLHYMLNKPVGVVSTASDPEGRQTVLDLLDLPSRVWPV
                     GRLDTDSEGLLVVTNDGELTHRLTHPRYEVPKTYLAEVKGTAGSRAIKQLIAGVELED
                     GMTKRAKVSLLEATRGGTL"
BASE COUNT          419 a          971 c         1002 g          362 t
ORIGIN      
        1 cgccagcgcg agctggtctc ggccgggctg tttctctatc cggtgctgca ggccgccgac
       61 gtcctcgcct accgggcgac cgaggtcccc gtgggggagg accagcgcca gcacatcgag
      121 ctgatgcgcg acgtcggggc ccggttcaac gcccagctcg gcgagacgtt ccggctgccc
      181 gagccgctca tccccgagaa gggggcccgg atcatggccc tcgacgaccc gacccagaag
      241 atgagcaagt ccgccggccg gcccgcgtcg tacatcggcc tggtcgagcc cgagaacgtc
      301 gtcgcgaaga aggtgaagtc ggcggtcacg gactcgggcc gcgaggtgcg ggcggccgac
      361 gacaagccgg cgctgacgaa cctgctcacg atcttctcgc tggtcgaggg gacgccggtg
      421 cccgagctcg aggagcggtt cgcctcggcc ggctacggcg acttcaagaa ggcgctcgcc
      481 gaggtcgtcg tcgagcggct ccggccgatc cgggagcgct acgaggagct ggtcggcgac
      541 ccggccgaga tggaggcgat cctgcggcgg ggggccgaga gggcggcagc cgaagccgag
      601 ctgacgatga aggtcgtcag ggaacggacg ggcctcctcc cggcctgacg ctgcgcacca
      661 agcccgagtt ggcccgacgg catcggcggg acgaacccgg ccccacggct ctagcgccgg
      721 ccgcaccaaa cccggccccc aacccgggcc gccgggtagg ctggagcccc cagcggggac
      781 gggaattgtc gacaagtcga ttcgtcgact agtggatcga gaggacgtgg ccgggacgtg
      841 gcgtacgagg tgaagctcga ggtcttcgag ggcccgatcg acctcctcct gcacctcatc
      901 acccggcagc gcgtcgacat ctacgacgtc tcgctcgccc ggatcaccga ggagtacctc
      961 gctgcgatct cggagctcga cgggaccgac ctcgagacgg ccaccggctt cctcgtcgtc
     1021 gcggcgactc tcctcgagct caagtccgca cggctcctgc ccggccgcac cggcgacgag
     1081 gaggagaccc acctcttgga ggagcgggac ctcctgctcg cacggctcgt cgagtgctcg
     1141 acgttccggg aggcgggggc gtggctcgcc cgggggctcg aggcgggttc cgccgcgcac
     1201 gggcgcgcgg ccgggctcga ggagaggttc gtcgacctcg ccccgaagat cgtcctcaag
     1261 acgacgcccg agcgcctcgg cgcgctggcg gccgccgccc tcgcgcccaa gcctgaacct
     1321 gaggtcgaca tgtcgcacgt ccagccgatc cgggcgacgg tgcgggacgc gatcctcgag
     1381 gtcgcctcgt acctgcggag cctcggggcc ccggccggct tccgcgagct ctgctcaggc
     1441 gccgacgagc ggatcgacgt cgtcgtgagg ttcctcgccc tgctcgagct cttcaaggcc
     1501 ggggccgtcg acctcgtcca ggtcgaccgc ttcggcgaca tccgcgccag ctggacgggc
     1561 gcggccgagg ccgacgaggt cctggccgac gccgacgagt acgtgatcga cctgagggag
     1621 gccggcagat gaacgacgca cccgagagcg cgcccgccac gaggaagatc gtcgaggcga
     1681 tcctgctgct cgcggacgag cccgtgccgg cgtcgcagat gggcgaggtc ctcgaggtcc
     1741 ctcgagccga cgtcgaggcg gtcctccgcg agctcgccga cgagtacgcc cgcgacgacc
     1801 ggggcttcgt gcttcgggag tccgcaccgg ggtggcgcct ctacaccgac cccgagtgcg
     1861 cgccgtggct cgagcgcttc gtcaccggcc atcgccacgc ccggctgagc ggggcgtccc
     1921 tcgaggtcct ggcgatcgtc gcctacaagg ggccgatctc ccgctccgag atctccgaga
     1981 tccgcggcgt cgactccgac ggggtcgtga agacgctgct gcagcgaggc ctcatcgaag
     2041 cttccggccc cggcggcccc ggcggccccg gcgcggcgaa cctgttcgag gtatccgacg
     2101 agttcctcga gcgcatgggg ctccgctcgg tcgatgacct tccgccgctc acggacttca
     2161 tgcccgacgc cgaggcggtc gaggacatgg aggcgaagct ctcgccgaac gcctgacgtt
     2221 gggcgacgag ggggagcggc tccagaaggt cctggcgcac gccggcctgg gatcgcggcg
     2281 ggcgatggag gacctgatcg cggccggaag ggtcaaggtc aatggccgcc gtgcgcagct
     2341 cggggcccgg gtcgaccccg acaaggatga agtagaggtc gacggttcac gcgtcccact
     2401 gcggcaggag ctcctccatt acatgctcaa caagccggtc ggcgtcgtca gcacggcgtc
     2461 ggaccccgag gggcgccaga ccgtcctcga cctgctcgac ctcccgagcc gggtgtggcc
     2521 agtcgggcgc ctcgacacgg acagcgaagg cctcctcgtg gtgacgaacg acggcgagct
     2581 cacgcaccgg ctgacgcacc cccgttacga ggtgccgaag acctacctcg ccgaggtgaa
     2641 ggggaccgcg gggagccggg cgatcaagca gctgatcgcc ggcgtcgagc tcgaggacgg
     2701 gatgacgaag cgggcgaagg tctcgctcct cgaggcgacc cgcgggggga cgct
//
LOCUS       DASQDO010000419         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_717803, whole genome shotgun sequence.
ACCESSION   DASQDO010000419 DASQDO010000000
VERSION     DASQDO010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717803"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VEV43_11705"
     CDS             complement(<1..698)
                     /locus_tag="VEV43_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24230.1"
                     /translation="MDALQRWLDGRRAGGLFAGFVVPLYVALATAAVAVFVVCSVWKG
                     LEPGANGECSLHVGAALAGSSDVFARDVAPGCSDLTLGEARATIRWDFAFILLYAPLA
                     TAALWWLWPRAWRVRRLQNFRWVAFTFTGAGLLDVVENALVLVALREGPALSEIPARA
                     ASVAGWWKWLLVVSGSLALLAAICGAIGNRRVPSEPHRERPPSPPGVPDGIGICLSGG
                     GIRSAGFALGALRAL"
     gene            complement(755..1495)
                     /locus_tag="VEV43_11710"
     CDS             complement(755..1495)
                     /locus_tag="VEV43_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HYP24231.1"
                     /translation="MTDVSFPIAFLAGIISFLSPCVLPLVPGYISYMSGVSAAAEGSP
                     AAPRRAAIAAGVFVLGFTVVFVPLGATATLLGSLLNDYQDQLRIASGVFIIFLGLLFV
                     GAFRIPALQREARFHPRPGSGLWGSAVLGGAFAFGWSPCIGPVLGSVLTMAAGRGSEG
                     GVGEGAALLATYSLGLGVPFVAAGLGITRLGGVVSWLRKHSRKVSVVSGVLLVGMGVL
                     FVTNQLFQISIWMQKTFVALNLDFLANI"
     gene            complement(1492..2028)
                     /locus_tag="VEV43_11715"
     CDS             complement(1492..2028)
                     /locus_tag="VEV43_11715"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYP24232.1"
                     /translation="MEAPKRRWVRVAWLLGPSIAFVLLLGAGIALKGDALEPGDAAPS
                     VEAPMLEGDGTLALDDLRGKPVLVNFWASWCVPCEAEARYLEAAHEEYGDEIDFVGVN
                     VKDARSDALAFVERYDVAYPQVRDEGLDWYDAFGLTGQPETFLIDAEGEVVEHVTGEF
                     TSTADLFRLLDVMVARDA"
     gene            2145..>2407
                     /locus_tag="VEV43_11720"
     CDS             2145..>2407
                     /locus_tag="VEV43_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYP24233.1"
                     /translation="MHHHGSTVLDSLTEPERERLLGRAVERRLGRGEALAHAGAPAER
                     VHVVTGGVVKLVGRDADGGETILGLCLPGEVAGAVAAVDARPHP"
BASE COUNT          415 a          885 c          832 g          275 t
ORIGIN      
        1 agcgcacgca acgccccgag cgcgaacccc gcggaacgga tccctccgcc agagagacag
       61 atgcctatgc cgtcggggac tccgggggga gacgggggtc tctcacgatg gggctcgctc
      121 ggcacgcgcc ggttcccgat cgcgccgcag atcgccgcca gcagcgcgag ggaacccgag
      181 acgaccagca gccacttcca ccatcccgcg accgaggccg cgcgcgcggg gatctccgac
      241 agcgccgggc cctcgcgcag agcgaccagc acgagcgcgt tctcgacgac gtcgagcagc
      301 ccggcccccg tgaacgtgaa cgcgacccag cggaaattct gcaaccgcct gacgcgccac
      361 gctcgcggcc acagccacca gagcgcggca gtcgcgagcg gcgcgtacag gaggatgaag
      421 gcgaagtccc accggatcgt cgctcgcgcc tcgccgagcg tcaggtcgct gcaacccggc
      481 gcgacgtcgc gagcgaagac gtcgctgctg cccgcgagcg cggcccccac gtgcagcgag
      541 cactcgccgt tcgcgccggg ctcgaggccc ttccacaccg agcagacgac gaagaccgcg
      601 acggcagcgg tggcgagcgc gacgtagagg ggcaccacga agccggcgaa cagcccgccc
      661 gcgcggcgac cgtcgagcca ccgctgcaga gcgtccacga tggctcgaat gatcgcagaa
      721 ggcccctgcg atcagccgtc tttgggcggc gccctcagat gttggcgagg aagtccaggt
      781 tgagtgccac gaacgtcttc tgcatccaga tcgagatctg gaacagctgg ttcgtcacga
      841 acaggacgcc catgcctacg agcagcacgc cgctcacgac gctcaccttg cgcgagtgct
      901 tcctcaacca agagacgacg ccgcccaagc gcgttatccc gagccccgcg gcgacgaacg
      961 ggacgccgag cccgagcgag tacgtggcga ggagcgcggc cccctcgccg actccgccct
     1021 cagatccgcg gccggccgcc atcgtcagca ccgacccgag gacggggccg atgcaggggc
     1081 tccacccgaa ggcgaacgcg cccccgagca cggcactccc ccacaggccg gagccggggc
     1141 gcgggtggaa ccgcgcctcg cgttgcaatg ccgggatacg gaacgcgccg acgaacagca
     1201 gcccgaggaa gatgatgaag acgccgctcg cgatcctcag ctggtcctgg tagtcgttca
     1261 gcagcgagcc cagcaaggtc gcggtagcgc cgagtgggac gaacacgacc gtgaacccga
     1321 gcacgaagac gccggccgcg attgctgcac ggcgcggcgc cgcaggcgat ccctcggcgg
     1381 ccgcggacac ccccgacatg tacgagatgt agccgggcac gagcgggagg acgcacggcg
     1441 acaagaacga gatgatgccg gcgaggaacg cgatcgggaa cgagacgtcg gtcacgcgtc
     1501 ccgcgccacc atcacgtcga ggagccggaa caagtcggcg gtgctcgtga actcgccggt
     1561 gacgtgctcg acgacctcgc cctcggcgtc gatgaggaac gtctcgggct ggcccgtgag
     1621 cccgaacgcg tcgtaccagt cgaggccctc gtccctcacc tgggggtagg cgacgtcgta
     1681 gcgctcgacg aacgcgagcg cgtcgctgcg cgcatccttc acgttgacgc cgacgaagtc
     1741 gatctcgtcg ccgtactcct cgtgggccgc ttcgaggtac cgggcctcgg cctcgcacgg
     1801 cacgcaccac gaggcccaga agttcacgag gacgggcttc cccctgaggt cgtcgagcgc
     1861 gagcgtcccg tcgccttcga gcatcggcgc ctcgaccgag ggggcggcgt cgccgggctc
     1921 gagggcgtcg cccttcagcg cgatccccgc gccgaggagg aggacgaacg cgatcgaggg
     1981 gccgagcagc catgcgaccc ggacccacct gcgcttggga gcttccacga acccattgtc
     2041 ccagcccccc gcggcgagat agcctgctcg tcgaggaggg gcaggcgacg cctgcccctc
     2101 gaccgcgccc agacctgtcc gtccgctacg gcaaggaggc gcgcgtgcac caccacggat
     2161 cgaccgtcct cgactctctg accgaacccg agcgcgaacg gctgctcggc cgcgccgtgg
     2221 aacggcgtct gggccgcggc gaggcccttg cgcatgcggg tgcccccgcc gagcgggtcc
     2281 acgtcgttac cggcggcgtc gtgaagctcg tgggccgtga cgccgacggc ggcgagacga
     2341 tcctcgggct ctgcctgccg ggcgaggtcg ccggtgctgt cgccgcggtc gacgcgcggc
     2401 cgcaccc
//
LOCUS       DASQDO010000420         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_657325, whole genome shotgun sequence.
ACCESSION   DASQDO010000420 DASQDO010000000
VERSION     DASQDO010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657325"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1250)
                     /locus_tag="VEV43_11725"
                     /pseudo
     CDS             complement(1..1250)
                     /locus_tag="VEV43_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648654.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(1340..1810)
                     /gene="rpsG"
                     /locus_tag="VEV43_11730"
     CDS             complement(1340..1810)
                     /gene="rpsG"
                     /locus_tag="VEV43_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012359569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HYP24234.1"
                     /translation="MPRKGPAARRELLPDPIYNSLLVTQIQNRIMTRGKRTLSEAIVY
                     DALRKVQDKTGKDPLPLLKKAVDNIRPLLEVRSRRVGGATYQVPVEVQSRRGGTLAIR
                     WLVTFSRKRREASMADRLAGEILDASGGAGAAVKRREDMHKMAEANKAFAHYRW"
     gene            complement(1810..2187)
                     /gene="rpsL"
                     /locus_tag="VEV43_11735"
     CDS             complement(1810..2187)
                     /gene="rpsL"
                     /locus_tag="VEV43_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HYP24235.1"
                     /translation="MPTIQQLVRTGRQSKREKSKSPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSGIEVTAYIPGIGHNLQEHSIVLVRGGRVKDLPGVRYKIIRAAL
                     DTAGVRDRVQSRSKYGAKKGGGA"
     gene            2644..>3165
                     /locus_tag="VEV43_11740"
     CDS             2644..>3165
                     /locus_tag="VEV43_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24236.1"
                     /translation="MFKSARSRVAVAVAGVVAASLAAGPLSGVGAAAKQTGLKDAGGR
                     AVAHLAGAKKISAPNTPTARWYDTTLDAGEPTIAHSKDGSVFFVGADIDTVGGRPNQV
                     DIVRSDDHGGTWTNVSPKVGPENAHPVTLDPYIWMDKDSGRPFTFELTVACSLLSFTD
                     DEGASWITNPLACG"
BASE COUNT          433 a         1078 c         1095 g          559 t
ORIGIN      
        1 ggcttcgact tcggctcgat cgcgacgtcg atgaccggcg tcgggaagac catctcctcg
       61 agcagcacgg ggtcgctcgg gtcgcacagc gtgtcgccgg tgagggtctg cttgaggccg
      121 acgatcgcca cgatgtcgcc cgcgaacacc gcgtccttgt cctcacggtg gttcgcgtgc
      181 atctggagga tgcggccgat gcgctccttg cggtcgcgca tcgagttcag gacgtggtcg
      241 ccgctcttga ggcttcccga gtagacgcgg gcgtaggtga ggcggccgac gtacgggtcg
      301 ctcatgatct tgaaggccag cgcggagaac ggcgcggagt cctcggccgg gcgctcgacg
      361 gtctcgccgg aaccgggcct cgtcgccacc atcggcggga ggtccagcgg gctcgggagg
      421 taccaggtga tggcgtcgag cagtggctgc acggccttgt tcttgaacgc cgtccctcac
      481 agcaccgggg tgatctcgcc cgacagcgtg ccccgacgga tggccttgcg gatgtgctcg
      541 acgtcggggt cctgctcggc gatgtagagc tccatcagct cctcgtcgtg gtcggcgagc
      601 gcctcgagca tgtcgtggcg gtactggtcc gcctgctcct tcatgtcgtc ggggatcggg
      661 cggtcctccc actcctcgcc catgccgtcc gacggccagt agtgggcgac catcgtgacg
      721 aggtcgacca cgccgtggaa gtccgcctcg cggccgatcg gcagctggat gacgaccggg
      781 gtgccgttga gacggctctc gatcatctcg acgcagcgga agaagtcggc accggtgcgg
      841 tccatcttgt tgacgaagca catgcgcggc acgttgtacc ggtcggcctg gcgccacacg
      901 gtctcggact ggggctcgac accggcgacg gcgtcgaaca cggccacggc gccgtcgagc
      961 acgcggaggc tgcgctcgac ctcgaccgtg aagtcgacgt ggccgggcgt gtcgatgatg
     1021 ttgatccagt agcccttcca gttcgcagtc gtcgcggccg acgtgatcgt gatcccgcgc
     1081 tcctgctcct ggaccatcca gtccatgacg gcggcgccct cgtggacctc gccgatcttg
     1141 taggtcttgc cggtgtagta gaggatccgc tcggtcgtcg tggtcttgcc cgcgtcgatg
     1201 tgagccatga tcccgatgtt gcgggtatgg ctcagggggt actcgcggat gctcggcgcg
     1261 ccgccgccgc ccttggtcgg gcgagcctgc ggtttgtcgg gtcgtgcctg ttgcgctacg
     1321 tcagccatct tctacgtgtt caccacctgt agtgggcgaa ggccttgttg gcctcggcca
     1381 tcttgtgcat gtcctcgcgg cgcttgaccg cggcccccgc gcctcccgac gcgtccagga
     1441 tctcgccggc gagccggtcc gccatcgacg cctcgcgccg cttgcgcgag aacgtgacga
     1501 gccagcggat ggcgagcgtc ccgccccttc tggactggac ctcgaccggg acctggtacg
     1561 tggcaccacc gacacgccgc gagcggacct cgagcagcgg ccggatgttg tcgaccgcct
     1621 tcttgaggag ggggagcggg tctttgcccg tcttgtcctg gaccttgcgg agggcgtcgt
     1681 agacgatcgc ctcggagagc gtccgcttgc ccctcgtcat gatccggttc tggatctgcg
     1741 tgacgagcag cgagttgtag atggggtccg gcagcagctc ccgccgcgcg gcgggtcctt
     1801 tcctcggcat caggcacctc cgcccttctt cgcgccgtac ttgctacggg actggacgcg
     1861 gtcgcgcacg ccggcggtgt cgagggcggc gcggatgatc ttgtagcgga cgcccggcag
     1921 gtccttcaca cggcccccgc ggacgagcac gatcgagtgc tcctggaggt tgtggccgat
     1981 gccggggatg taggcggtga cttcgatccc ggaggtgaga cggacacggg cgaccttgcg
     2041 aagcgccgag ttcggcttct tcggcgtggt cgtgtagacg cgggtgcaga cgcccctgcg
     2101 ctgcggcgac cccttgaggg ccggtgactt cgacttctct cgcttgctct ggcggcccgt
     2161 acggaccagc tgctggatcg tcggcaactc gtctacctcc cggccggcgc cgtttcgcct
     2221 cgcggctctc aggcggcggc catgtctcgt tcccggcgga tgccgggctg tctcacctct
     2281 atgggccccc gcgccgaata cggcgccgcg accttttgtg ccaagcgtcg aggagatatg
     2341 gggcccggcc tgtgacgagg cgggcgtcct cccgacggct tctcgttcag cgatgaaccc
     2401 cgacgcctgc ggttcccggg gcaaccgggc ctgcaggagc tcgccgaact cgggtcgttc
     2461 cattcggttc ccgcgaatca aagccagacg cgggatgcgc ccccgaaggg acgcagcggc
     2521 aaaggatagg ccaggtgtcc gacctggtca aacgggtcac ctccttgctc acccggcagg
     2581 gggaggcagg tgcgtggcga acccgtgacc aagactgact gccaacttct acgggggttc
     2641 ttcgtgttca agagtgccag gagcagagtc gccgtcgccg tcgccggcgt cgtggccgcc
     2701 tcgctcgccg ccgggcccct ttcgggggtc ggcgccgccg cgaagcagac cgggctcaag
     2761 gacgccgggg gccgggccgt cgcccacctc gccggcgcca agaagatctc cgccccgaac
     2821 acccccaccg cccgctggta cgacacgacg ctcgacgccg gcgagcccac gatcgctcac
     2881 tccaaggacg ggtcggtctt cttcgtcggc gccgacatcg acaccgtcgg cggccgcccg
     2941 aaccaggtcg acatcgtgcg ctcggacgac cacggcggca cctggacgaa cgtctcgccg
     3001 aaggtcgggc cggagaacgc ccacccggtc acgctcgacc cgtacatctg gatggacaag
     3061 gactcgggcc ggcccttcac gttcgagttg accgtggcct gctcgctcct atcgttcacc
     3121 gacgacgagg gggccagctg gatcacgaac ccgctggcct gcggc
//
LOCUS       DASQDO010000421         5899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_962863, whole genome shotgun sequence.
ACCESSION   DASQDO010000421 DASQDO010000000
VERSION     DASQDO010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962863"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1555)
                     /gene="murC"
                     /locus_tag="VEV43_11745"
     CDS             complement(173..1555)
                     /gene="murC"
                     /locus_tag="VEV43_11745"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HYP24237.1"
                     /translation="MGDLAFRPGSSVHFVGIGGAGMSAIAKVLLERGVTVSGSDLKAS
                     RPATLLEAMGARVAIGHDAAHVSGADVVVVSSAIPERNPELRAARDAGLRVAGRGEAL
                     AAVLRDQRSIVVAGTHGKTTTTSMIVSVLRAAGRDPTYLVGGGLNDAGTNAKSGRDDL
                     AVAESDESDGSFLLLRPAIAVVTNVEPDHVDYWESEEALLAAFEQFVASTDPSGTVVA
                     PAAQPEVVAWAQEGPAAHVTFGEGGDVAATSVRFGSGEVAFDLEADGERHPVHLRVPG
                     HHNVMNALAAAAACRAAGLPVAEVAAGLGEYRGVERRWHMRGEARGVTVIDDYAHHPT
                     EVSATLGAARPGPWGRVVAVFQPHRYSRTRAFAGEFGASFAGADRVVVTDVYGAGEEP
                     VPGVTGKLVADAVCRAFPGRPVAYLPHRDELVAYLAATSKPGDAVLTLGAGDVTAVGE
                     ELLERIASAR"
     gene            complement(1556..2539)
                     /locus_tag="VEV43_11750"
     CDS             complement(1556..2539)
                     /locus_tag="VEV43_11750"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYP24238.1"
                     /translation="MGSWDGRKVMVTGGEGFIGSNLVDLLLHEGADVRCLVHYNPGGT
                     WGWLGRVEDDVEIVPGDVRDGERVQQAVEGRDVVLHLAALIGIPYSYGAPESYVQTNV
                     LGTYNILNAARRHETPRVVCTSTSEVYGTARTVPIDESHPLQPQSPYAATKIGADALA
                     ISFHRSFELPVAIIRPFNTYGPRQSTRAVIPMIIGQLYGDAPEVKLGALSPTRDFNYV
                     EDTARGFMAIADSDRALGDVVNIGTGREVSIGETVELLKQVTGIDKPVVSDEERMRPG
                     GSEVHRLLCDNTRAREWTGWEPQVTLEEGLRRTAEWVRENPGALKTKKYHV"
     gene            complement(2539..3591)
                     /locus_tag="VEV43_11755"
     CDS             complement(2539..3591)
                     /locus_tag="VEV43_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYP24239.1"
                     /translation="MRESDRASHVLRPENTLDEAMRALQSNREGVAVVVDGDDRMLGT
                     VIDADIRRATLNDISLSAPVTAVMSRNPVRAESGATKSELLDLMRLHRLRAIPIVDDE
                     VLVDVRSLTSVVEDEILPVTVIMAGGEGRRLRPLTETVPKPLVPIGDRPVVEGVIGPL
                     RRAGVKDITLALNYKAELIQEHLGTGERLGVSLRYMIEKERMGTAGALSLLPETPDRP
                     ICVSNADIVTTMSFARLFDYHWHHRAAITVAATWHTSAIPYGVLRTAGHYMLGMIEKP
                     EQRELVSGGIYVLDPEVLRFIRGRAPLDMPDLVADVLAEGLPVCVFPILETWVDIGRI
                     EDLERARAALQLSEED"
     gene            complement(3588..4718)
                     /gene="murG"
                     /locus_tag="VEV43_11760"
     CDS             complement(3588..4718)
                     /gene="murG"
                     /locus_tag="VEV43_11760"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018535419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HYP24240.1"
                     /translation="MTNVVIGAGGTGGHIFPGLALAGAIEEAEPGARVDFVGTERGLE
                     KTLIPNAGYPLHMIDVLPLQRKLGIEPLLAGPALVRATWQARRLLRSLGADVAVGMGG
                     YPSLPVIVAASSMRLPCLLHESGAVAGLANKIAGRLTPNVALSFDEAASAFKRPTRTT
                     GMPLSGAIAHMDRAALRAEARAAYGLDDDRVAVLVMGGSLGALSLNELALGLAARWKA
                     RDDVRIILKAGRGHLEAAQKKLSELGAGHVVNAMEFLDRMELAYAAADVAITRAGAGT
                     VAELPAAGLPAVLIPDPHAPGDHQTLNAQPLVEAGAAYVVADAEARPERVGPLLEELI
                     DDRARLAAMGEKAKGLARPRAAADLAAWVLELAGRGRRRKKR"
     gene            complement(4732..>5899)
                     /gene="ftsW"
                     /locus_tag="VEV43_11765"
     CDS             complement(4732..>5899)
                     /gene="ftsW"
                     /locus_tag="VEV43_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HYP24241.1"
                     /translation="TRSGTLGVARRGSRAKPRNAAAVRPVSWSDPAGLLMTTTGVLVL
                     VGLVMILSASLWNSEASPFLYFNKQALWATIGLTGFFVFSRYDYKRLKGLGYKLLPFV
                     VVMLLVVLIPGIGTNVGGSSRWFRLGPLSFQPSEAAKLALILFAADVFSRKKEAKFKD
                     FAHAALPMIPVMGLLAALVMLQPDLGTTSLLGFIGFGMLFVAGARLRHLLPFAAAGGA
                     LALGAALFEPYRRARILAFLNPWADPLNTGYHTIQSLIALGSGGWFGVGLGASRQKWT
                     YVPNAHTDFIFAILGEEMGLLGTLVIVGLFAFLGYLGITTARRAPDRFGMLLAAGITI
                     WITLQALVNMGAVTAALPITGVPLPLVSFGGTSLVICLLAMGILTNIARQGRRA"
BASE COUNT          846 a         2146 c         2090 g          817 t
ORIGIN      
        1 cccgcctcgc gcagcaggtc cgcgaccacc gtgaggtcgt cgggggccgc aggttcgacg
       61 aggagcgcgg cgggcccgcc gagccggtac gtcgtgagtg gcgcgagcga cgccccgcgc
      121 tcgacgcgcc cgggcagtgc ctcttcgaga gcccgtgcga gcgcctcgat cgtcatcgcg
      181 cggaggcgat cctctcgagg agctcctccc ccaccgcggt gacgtcgccc gccccgagcg
      241 tgagcaccgc gtctccgggc ttggacgtcg ccgcgagata cgcgaccagc tcgtcccggt
      301 gcgggagata cgcgaccggg cgccccggga aggcgcggca caccgcgtcg gcgacgagct
      361 tgcccgtcac gcccggcacg ggctcctccc ccgcgccgta gacgtcggtg acgacgacgc
      421 ggtcggcacc cgcgaacgac gcgccgaact cgccggcgaa cgcgcgtgtg cgcgagtaac
      481 gatgcggctg gaagacggct acgacgcggc cccacgggcc cgggcgcgcg gcgcccaagg
      541 tcgcgctcac ctcggtcgga tggtgggcgt agtcgtcgat cacggtgact ccccgggcct
      601 cgccgcgcat gtgccagcgg cgctcgacgc cgcggtactc gccgagcccc gccgcgacct
      661 cggccaccgg cagcccggcc gcacggcacg cggccgcggc ggccagagcg ttcatcacgt
      721 tgtggtggcc gggcacgcgc aggtgcaccg ggtggcgttc gccgtccgct tcgaggtcga
      781 acgcgacctc gccggagccg aaccggaccg aggtggccgc cacgtcgccg ccctcgccga
      841 acgtcacgtg tgcagcaggc ccttcctgag cccacgccac gacctcgggc tgcgcggccg
      901 gagcgaccac ggtcccggac ggatccgtcg aggcgacgaa ctgctcgaac gccgccagca
      961 gagcttcctc ggactcccag tagtcgacgt ggtcgggctc gacgttcgtc acgacggcga
     1021 tcgccggtct cagcagcagg aaggacccgt cgctctcgtc ggactcggcc acggcgaggt
     1081 cgtcccgccc ggacttcgcg ttcgtcccgg cgtcgttgag ccccccgccg accagatagg
     1141 tcgggtcgcg ccccgcggcg cgcagcaccg acacgatcat cgaggtagtc gtggtcttcc
     1201 cgtgcgtgcc cgcgacgacg atcgagcgct ggtcgcggag caccgcggcc agcgcctcgc
     1261 ctcggcccgc cacgcggagc ccggcgtcgc gcgcagcgcg cagctccggg ttccgctcgg
     1321 ggatcgccga cgacaccacg acgacgtccg cgccggagac gtgcgcggcg tcgtggccga
     1381 tcgcgacgcg ggcgcccatg gcctcgagca acgtcgccgg ccgggacgcc ttcaggtcgg
     1441 acccggagac cgtgacgccg cgttcgagga gcaccttcgc gatcgccgac atgccggccc
     1501 cgccgatgcc cacgaagtgg accgacgagc cggggcggaa cgcgaggtcc cccatctaca
     1561 cgtggtactt cttggtcttc agcgcgccgg ggttctcgcg gacccactcg gccgtccggc
     1621 gcagcccctc ctcgagcgtc acctgcggct cccaccccgt ccactcacgg gcccgcgtgt
     1681 tgtcgcacag cagccggtgg acctcgctgc cgcctggccg catgcgctcc tcgtcggaga
     1741 cgacgggctt gtcgatcccc gtcacctgct tcaggagctc gacggtctcg ccgatcgaga
     1801 cctcgcgccc ggtgccgatg ttcacgacgt cgccgagggc ccggtcggag tccgcgatcg
     1861 ccatgaagcc gcgagcggtg tcctcgacgt agttgaagtc gcgcgtcggg ctgagagcgc
     1921 cgagcttgac ctcgggcgcg tcgccgtaga gctggccgat gatcatcggg atcaccgcgc
     1981 gcgtggactg gcgcgggccg taggtgttga agggcctgat gatcgcgacg ggcagctcga
     2041 aagagcggtg gaagctgatc gcgagagcgt cggcgccgat cttcgtcgcc gcgtacgggg
     2101 actggggctg caaggggtgc gactcgtcga tcggcaccgt ccgcgccgtc ccgtagacct
     2161 cgctcgtcga cgtgcacacg acacgagggg tctcgtgccg gcgcgccgcg ttgaggatgt
     2221 tgtacgtgcc gaggacgttc gtctgcacgt agctctccgg cgccccgtag ctgtacggga
     2281 tgccgatcag cgccgcgagg tgcagcacga cgtcgcggcc ctcgaccgcc tgctggaccc
     2341 gttccccgtc gcgcacgtcg cccggaacga tctcgacgtc gtcctcgacg cggccgagcc
     2401 acccccacgt cccgccgggg ttgtagtgga cgaggcagcg gacgtccgcg ccctcgtgga
     2461 gcagcaggtc gacgaggttg ctccctatga agccctcgcc gcccgtcacc atcaccttgc
     2521 gtccgtccca ggaacccatc agtcctcctc gctcagctgg agcgccgccc gcgcgcgctc
     2581 gagatcctcg atgcgtccga tgtcgaccca cgtctccagg atcgggaaca cgcagacggg
     2641 gagcccctcc gccagcacgt ccgcgaccag gtcgggcatg tcgagaggag cgcgcccgcg
     2701 gatgaagcgc agcacctccg ggtcgagcac gtagatgcca ccactgacga gctcgcgctg
     2761 ctcgggcttc tcgatcatcc cgagcatgta gtggccggcc gtccgcagca caccgtaggg
     2821 gatggccgac gtgtgccagg tcgcggcgac cgtgatcgcc gcgcggtggt gccagtggta
     2881 gtcgaacagg cgcgcgaagc tcatcgtcgt gacgatgtcc gcgttcgaga cgcagatcgg
     2941 gcggtcgggt gtctccggca gcagcgacag cgcgccggcg gtccccatcc gctccttctc
     3001 gatcatgtag cgcagcgaca cgccgagacg ctcgcccgtt cccaggtgct cctggatgag
     3061 ctccgccttg tagttgagcg cgagcgtgat gtccttgacg cccgcgcggc gcagcgggcc
     3121 gatcacgccc tcgacgacgg gtcggtcgcc gatgggcacg agcggcttcg ggaccgtctc
     3181 ggtcagcggg cggagcctgc ggccctcgcc tccggccatg atcacggtca ccggcaggat
     3241 ctcgtcctcg accacgctcg tgagggacct cacgtcgacg aggacttcgt cgtccacgat
     3301 cgggatcgcc ctcagccggt gcagcctcat gaggtccagg agctcgctct tcgtcgcgcc
     3361 ggactcggcg cgcacggggt tgcgcgacat cacggcggtg accggcgccg acagcgagat
     3421 gtcgttgagc gtcgcgcgtc ggatgtcggc gtcgatgacg gtccccagca tgcggtcgtc
     3481 gccgtccacg acgacggcga cgccttcgcg gttcgactgc agcgcgcgca tcgcctcgtc
     3541 cagcgtgttc tccggacgca gcacgtggga agctctgtcg ctttctctca tcgcttcttc
     3601 cttcgtcgtc cccgcccggc gagctcgagg acccaggccg ccaggtccgc ggctgcccga
     3661 ggcctcgcga gccccttcgc cttctcgccc atcgctgcga gccgggctcg gtcgtcgatc
     3721 agctcctcga ggagcgggcc gacgcgctcg ggccgcgcct ctgcgtctgc gacgacgtag
     3781 gcggcgcctg cctctaccag gggctgggcg ttcagggtct ggtggtcgcc cggggcatgg
     3841 ggatcgggga tgaggaccgc cggcagaccc gcggccggga gctcggcgac ggtcccggcc
     3901 cccgccctcg tgatcgcgac gtcggcggcc gcgtaggcga gctccatccg gtcgaggaac
     3961 tccatcgcgt tgacgacgtg gccggccccc agctccgaga gcttcttctg cgcggcttcg
     4021 agatgccctc gcccggcctt caggatgatc cggacgtcat cgcgcgcctt ccagcgcgcg
     4081 gcgaggccga gcgcgagctc gttgaggctc agcgccccga ggctgccccc cataacgagc
     4141 accgcgacgc gatcgtcgtc gaggccgtac gccgcgcgcg cctccgcgcg gagggcggcg
     4201 cggtccatgt gcgcgatggc gccgctcagc ggcatgccgg tcgtccgcgt cggccgcttg
     4261 aacgcgctcg cggcctcgtc gaacgagagc gcgacgttcg gcgtgagccg tccggcgatc
     4321 ttgttcgcaa ggccggcgac ggcgcccgac tcgtgcagca ggcaggggag gcgcatcgag
     4381 gacgcggcga cgatcaccgg caggctcggg tagcccccca tgccgacggc cacgtcggcg
     4441 ccgagcgacc gcagcagccg ccgcgcctgc caggtcgcgc gcacgagcgc cggcccggcc
     4501 agcagcggct cgatcccgag cttcctctgg agcggcagca cgtcgatcat gtgcagcgga
     4561 tatccggcgt tcgggatcaa ggtcttctcg agcccgcgct cggtcccgac gaagtcgacc
     4621 cgggcccccg gctccgcctc ttcgatcgca ccggcgagcg cgagccccgg gaagatgtgc
     4681 ccgccggtcc ccccggcccc gattacgacg ttggtcacgc ggtcgaggcg gtcaggcgcg
     4741 tcgaccctgc cgcgcgatgt tcgtgaggat ccccatcgcg aggaggcaga tgacgagcga
     4801 ggtcccgccg aacgacacga gcgggagggg cactccggtg atcggcaacg cggctgtgac
     4861 cgcgcccatg ttcacgaggg cctggagcgt gatccagatc gtgatccccg ccgcgagcag
     4921 catcccgaag cggtcgggag cgcggcgcgc cgtcgtgatg ccgaggtacc cgaggaacgc
     4981 gaacagcccg acgatcacga gcgtgcccag cagccccatc tcctcgccga ggatggcgaa
     5041 gatgaagtcc gtgtgcgcgt tggggacgta ggtccacttc tgccggctcg cgccgaggcc
     5101 gacgccgaac catccaccgg acccgagcgc gatcagcgac tggatcgtgt ggtagccggt
     5161 gttgagcggg tcggcccagg ggttgaggaa cgcgaggatg cgcgcccggc ggtacggctc
     5221 gaacagcgcg gcccccagcg cgagagcgcc gccggcggcc gcgaacggga gcaggtgccg
     5281 cagacgcgcg ccggccacga acagcatgcc gaagccgatg aacccgagca gcgacgtcgt
     5341 cccgaggtcg ggctggagca tcacgagcgc ggccaacagc cccatgaccg ggatcatcgg
     5401 gagcgcggcg tgggcgaagt ccttgaactt cgcctccttc ttgcgcgaga agacgtcggc
     5461 ggcgaacagg atcagcgcga gcttggccgc ctcggacggc tggaacgaga gcgggccgag
     5521 tcggaaccag cgcgagctcc cgcctacgtt cgtgccgatg cccgggatca gcacgacgag
     5581 cagcatcacg acgacgaacg gcaggagctt gtagccgagc cccttcagac gcttgtagtc
     5641 gtaacgcgag aagacgaaga agccggtgag gccgatcgtg gcccacaacg cctgcttgtt
     5701 gaagtagagg aacggcgacg cctcgctgtt ccagaggctc gccgacagga tcatcacgag
     5761 gccgacgagc acgaggacgc cggtcgtcgt catcagcagg ccggcagggt cggaccacga
     5821 caccgggcgc accgcggcgg cgttgcgcgg cttcgcgcgg gagccgcggc gggcgacccc
     5881 gagcgtgccg ctccgcgtc
//
LOCUS       DASQDO010000422         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_290384, whole genome shotgun sequence.
ACCESSION   DASQDO010000422 DASQDO010000000
VERSION     DASQDO010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290384"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..1529
                     /locus_tag="VEV43_11770"
     CDS             462..1529
                     /locus_tag="VEV43_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYP24242.1"
                     /translation="MIVLPVEEQAAAPAVTSTEGDLRVVWTPRAVEDASSAVALLPRR
                     ARPPWVRPDVLVVGEELPSYLRGEKGFHLLDAHGVRDLLAAPMRTRNGWGARREFAPI
                     VAVPDSFECDASLLADLRRLEEAGVPVFPLSAVARMRCRLELLDGDPPAPRSSRISAG
                     LHRALDLAVALAGATATIAVLPFVAAALWIEDRGPVFYMQERIGRGGRTFTLLKFRSM
                     RPDAERSGPAWATSDDARTTRVGAFLRRFKIDELPQFFNVLVGHISLVGPRPERPFFA
                     EILKTHVPHYDVRHEVRPGLTGWGTVKVGYGNSIEAKYLAHQFDLYHLTNRSLRFDLE
                     IMARSARFLVLPPEVADRYML"
     gene            1535..1867
                     /locus_tag="VEV43_11775"
     CDS             1535..1867
                     /locus_tag="VEV43_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HYP24243.1"
                     /translation="MARDPASEDDAITVPLDYRPRKRADVLELDMGDGFILYNHDSSL
                     VHHLNPTAAVVWQVLDGAATVEQLADEVADEYGLPRDNVLVQITSLIAELDAMGLVED
                     ASPEASSD"
     gene            1872..3605
                     /locus_tag="VEV43_11780"
     CDS             1872..3605
                     /locus_tag="VEV43_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24244.1"
                     /translation="MAVPESRLSRGVALGDYVRMIWQRRWIVVVAVVVATILAVVTRP
                     PEATALYSATATLRVQVFGFTASGEDVLTAQEGIPPAEVEAVRSVVVAAETAEELGMS
                     DGGAELLRQLGVGAEENTDLLRITLTGEGLDTVETLEAYANNYVEYRAEQDVERLERA
                     LADVNASIREVDAQLARFSARLAVEEAAGGASPETQTKYDTYSAIYTRLLGTKQAFKL
                     EASLAGNQVEVVGSPIVQRVGAIPARTIRLFAGQLVGLLLGCAIAVAIGVLRPRISGR
                     DRTEEQLGYPVLAVVPRVRNRRIEKDPLVVQRASGWGAEGVRMLRTELQLVEERGAEL
                     KVVVVASPEPRDGKSTVSSNLAASYALSGRNAVLVHADLRADRRHRDPGGPGLADYVA
                     GELAEVPVHRSPGGFAEVHAGSARYQGGVVQERLVDAIRTLRAEYDVVVVDTPPLLAF
                     ADALLLAAEADAVVVVVRDGKTLEDKAGEALEVLVRHDAAVAGLVLNDARVGRLERRR
                     YRRYYGSWTELEAPPVAAEEAPPPPPPVEPEAAEDEAVEQVASLRHEPEDAPRPLFRR
                     RGGERAVRSRG"
BASE COUNT          537 a         1352 c         1329 g          520 t
ORIGIN      
        1 ggatcgccgt cgacccctcg aaggcgggcc gcgagctggg atggtcgccg caggtcgggc
       61 tcgacgacgg gctcaagcag accgtcgcgt ggttccgcgc cacgagctaa cgaactccca
      121 aattcggcgt taacgtcatc aaaggggcgt cgcgccggcc aaattcgtcg aattcccaag
      181 aaaaaacagt ggaaggattt ggctggaaga cataggctgt ggaccaccca atcgggcggg
      241 tcggaacccc cggacgaggt atcgtccgac tccggtccgc ggcggcccgg tggcggagag
      301 taaggcaagc tcggcggcgc cgggcggcgc gggacactag gcgcgggagg aaaggtttgg
      361 ccttcgagac ttcgagtccc gagacgcacg cggccacccg agcccctgcc aacggccgtc
      421 accctcgcga agcgcacgac tccgtcgctg aggagcaccg tgtgatcgtc ctcccggtcg
      481 aagagcaggc cgcggccccc gcagtcacct cgacggaagg cgacctgagg gtcgtctgga
      541 ccccgcgggc ggtcgaggac gcgtcgtcgg cggtagcgct gttgccgcgg cgggcgcggc
      601 ccccctgggt gcgcccggac gtgctcgtcg taggcgagga gcttccttcg tacctgcgcg
      661 gcgagaaggg cttccacctc ctcgacgctc acggcgtaag agacctgctc gcggcgccga
      721 tgcgcacccg caacggatgg ggagcacgcc gcgagttcgc gccgatcgtc gccgtccccg
      781 actcgttcga gtgcgacgcg tcgctgctcg ccgacctccg gaggctcgaa gaggccggcg
      841 tcccggtctt cccgttgtcg gcggtcgctc gcatgcgctg ccggctcgag ctcctcgacg
      901 gcgaccctcc cgctccccgt tcctcacgca tctccgcagg cctgcaccgc gcgctcgacc
      961 tcgcggtcgc gctcgcgggt gccacggcca cgatcgccgt gctcccgttc gtcgcggccg
     1021 cgctctggat cgaggaccgc gggcccgtct tctacatgca ggagcggatc gggcgcgggg
     1081 gccggacctt cacgctgctg aagttccgga gcatgcgtcc ggacgccgag cggtcgggcc
     1141 ccgcgtgggc cacgtccgac gacgcgcgga ccacgcgggt cggcgcgttc ctgcggcgct
     1201 tcaagatcga cgagctgccg cagttcttca acgtgctcgt gggccacatt agcctggtgg
     1261 gaccgcgccc cgagcgcccg ttcttcgccg agatcctgaa gacgcacgtg ccgcactacg
     1321 acgtccggca cgaggtgcgg ccggggctca ccgggtgggg caccgtcaag gtcggctacg
     1381 ggaactcgat cgaggcgaag tacctcgcgc accagttcga cctgtaccac ctgacgaacc
     1441 gctcgctgcg cttcgacctc gagatcatgg cgcgcagcgc gcgcttcctc gtgctgccac
     1501 ccgaagtcgc ggataggtac atgctgtgag tcagatggca cgcgaccccg catccgagga
     1561 cgacgcgatc acggtccctc tcgactaccg cccgcgcaag cgcgcggacg tgctcgagct
     1621 ggacatgggt gacggcttca tcctctacaa ccacgactct tcgctcgtcc atcacctgaa
     1681 cccgacggcc gccgtcgtct ggcaggtcct ggatggcgcg gcgaccgtcg agcagctcgc
     1741 ggacgaggtc gcggacgaat acggacttcc gagggacaat gtgctcgtgc agatcaccag
     1801 tctgatcgcc gagctggacg ccatgggctt ggtcgaggac gcgagcccgg aagcttcctc
     1861 cgactagggc catggccgta cccgagtccc ggctgagccg gggggtcgcc ctcggcgact
     1921 acgtccgcat gatctggcaa cggcgctgga tcgtcgtcgt cgcggtcgtc gtcgccacga
     1981 tcctcgccgt cgtcacgcgc ccgcccgagg ccaccgcgct ctacagcgcg accgcgaccc
     2041 tgcgcgtgca ggtgttcggg ttcaccgcgt ccggtgaaga cgtcctcacg gcgcaggagg
     2101 ggatcccgcc cgccgaggtc gaggccgtgc gctcggtcgt cgtcgccgcc gagacggcgg
     2161 aagagctggg gatgagcgac gggggggccg agctcctccg gcagctcggc gtgggcgcgg
     2221 aggagaacac cgacctgttg cgcatcacgc tcaccggcga gggcctcgac accgtcgaga
     2281 cgctcgaggc ctacgcgaac aactacgtcg agtaccgcgc cgagcaggac gtcgagcggc
     2341 tcgagcgcgc gctggcggac gtgaacgcga gcatccgcga ggtcgacgca cagctcgcgc
     2401 gcttctcggc gcgtctcgcc gtcgaggagg ccgccggcgg tgcgtcgccg gagacgcaga
     2461 cgaagtacga cacgtacagc gcgatctaca cgcgcctcct cggcaccaag caggcgttca
     2521 agctcgaggc cagcctcgcc gggaaccagg tcgaggtcgt ggggtcgccg atcgtgcagc
     2581 gcgtcggcgc gatccccgcg cgcacgatcc ggctgttcgc gggccagctc gtcggcctgc
     2641 tgctcgggtg cgcgatcgcc gtcgccatcg gcgtgctgcg cccgcggatc tcgggccgcg
     2701 accgtacgga ggagcagctc ggatacccgg tgctcgccgt cgtcccgcgc gtccggaacc
     2761 gcaggatcga gaaggacccc ctcgtcgtgc agcgcgcgtc gggatggggg gccgaaggcg
     2821 tgcgcatgct gcgcaccgag ctgcagctcg tcgaggagcg cggcgccgag ctcaaggtcg
     2881 tcgtggtcgc gagccccgag ccccgcgacg gcaagtcgac ggtgtcgagc aacctcgccg
     2941 cgtcctacgc gctctccggc cgcaacgccg tcctcgtgca cgccgacctg cgcgccgacc
     3001 ggcgccaccg cgacccgggc ggtcccggtc tcgccgacta cgtcgcgggc gagctggccg
     3061 aggtgccggt ccaccgcagc cccggcggct tcgccgaggt ccacgccggg tcggcgaggt
     3121 atcagggggg cgtggtccag gaacgcctcg tcgacgcgat ccggacgcta cgcgccgagt
     3181 acgacgtcgt cgtggtcgac acgccgccgc tgctcgcgtt cgccgacgcc ctgctgctgg
     3241 cggccgaggc cgacgcggtc gtggtcgtcg tccgcgacgg caagacgctc gaggacaagg
     3301 ccggcgaggc gctggaggtg ctcgtgaggc acgacgccgc ggtcgcggga ctcgtgctca
     3361 acgacgcccg cgtgggcagg ctcgagcgcc gcaggtaccg ccgctactac gggtcgtgga
     3421 ccgagctcga ggcccccccg gtcgccgccg aggaagcgcc gccgccaccg cctcccgtcg
     3481 agccggaagc ggccgaagac gaggccgtcg agcaggtcgc ctcgttgcgt cacgagccgg
     3541 aggacgctcc tcgcccgctg ttccgccgcc gcggcggcga gagggcggtc cgttcgcgcg
     3601 gctgaccgct cagcggaaca gcgcgtcgag gatctccccg ccccaccgct ccggcgacag
     3661 ctcctgctcc cacgtctgcg ccgcgcccgc ggcgagatgc cgcgcgaggc cggggtcggc
     3721 gtgcagcttc tgcatcgc
//
LOCUS       DASQDO010000423         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_473342, whole genome shotgun sequence.
ACCESSION   DASQDO010000423 DASQDO010000000
VERSION     DASQDO010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473342"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VEV43_11785"
     CDS             complement(<1..452)
                     /locus_tag="VEV43_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24245.1"
                     /translation="MRRRSAVLLVVLVFGALVASAQARAAASTYSDLSGDGEYGSLVS
                     PDIAGVRIELLSDGMLDIDVPIVNEPTQLARSEVNVFLNTDRSRVTGSGGYDFLIKVK
                     GPDSTPRLDRWANGWTTVPAPSLRVAWSSGPSIDLSSLELGGVTSFDFL"
     gene            575..>1823
                     /locus_tag="VEV43_11790"
     CDS             575..>1823
                     /locus_tag="VEV43_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYP24246.1"
                     /translation="MALDPKVFKAYDVRGIYPTELDEEGARKIGRAYVEVFSPRRIAV
                     GRALRVSSPAMAEAAILGATEAGADVVDIGMVGTEMLYFAVGDLGLDGGLTVTASHNP
                     KEYTGMKIVRRGALPVGGDSGLLEIRDRAEAPGEAGRSGTVERRDVYPGFVEKVLSFV
                     DVGAIRPLRVVIDAANGMAGAMLPPILDRLPVEAIRCYFEPDGSFPNHEPNPLLPENR
                     EFIVAKVREEGADLGIAFDGDADRCFFVDDTGEFVPGDFVTALLAELMLDKAPGSKVI
                     YDVRASWAVAHAVERAGGVPLVNRVGHAFIKHRMREEDAVFAGEVSGHYYFRDFCQAD
                     TGTVPALLLLEHVSKAGVPLSELLRPYRERYFITGELNTRVRDVALKLQEVKERFGRE
                     GRVSHIDGLEVTADDWHLNLRPSN"
BASE COUNT          279 a          644 c          625 g          275 t
ORIGIN      
        1 aggaagtcga acgacgtcac gccgccgagc tcgagcgagc tcaggtcgat gctcgggccg
       61 ctgctccacg cgacccggag agacggcgcg ggcacggtcg tccagccgtt cgcccagcgg
      121 tcgagccgcg gcgtcgagtc cggccccttc accttgatca ggaagtcgta gccgccgctg
      181 ccggtcaccc tggagcggtc ggtgttcagg aacacgttca cctcgcttcg cgcgagctgc
      241 gtgggctcgt tcacgatcgg gacgtcgatg tccagcatcc cgtccgacag gagttcgatc
      301 cgtacgcccg cgatgtcggg cgagacgagg gagccgtact cgccgtcgcc gctcaggtcg
      361 gagtaggtcg aagccgcggc ccgcgcctgc gcggacgcga cgagcgctcc gaacacgagg
      421 acgaccaaga ggacagcaga acggcgcctc atcgacttct cccttcggcc gagaccaatt
      481 tccgcggatc cgttctatct tctcacacgg acacgaccgc gcgaaggtcg atctccgatg
      541 ggggtatcgc ggccccggca tagactccgc gcccgtggcg ctcgatccca aggtgttcaa
      601 ggcgtacgac gtccgcggga tctaccccac tgagctcgac gaggagggtg cccggaagat
      661 cggacgcgcc tacgtcgagg tgttcagccc gcgccggatc gccgtcggcc gcgccctgcg
      721 ggtctcctcg cccgcgatgg cggaggccgc gatcctgggc gcgaccgagg cgggcgcgga
      781 cgtcgtcgac atcgggatgg tgggcaccga gatgctctat ttcgccgtgg gcgacctcgg
      841 cctcgacggc ggcctcacgg tgacggcctc gcacaacccg aaggagtaca cgggcatgaa
      901 gatcgtccgc aggggcgcgc ttcccgtcgg cggcgactcc ggcctcctcg agatccggga
      961 ccgcgcggag gccccgggcg aggcgggcag gagcggaacc gtcgagcgtc gcgacgtcta
     1021 cccggggttc gtcgagaagg tcctctcctt cgtcgacgtc ggcgcgatcc gcccgctccg
     1081 ggtcgtcatc gacgcggcga acggcatggc cggcgcaatg cttccgccga tcctcgaccg
     1141 gctgcccgtc gaggcgatcc ggtgctactt cgagcccgac ggcagcttcc cgaaccacga
     1201 gccgaacccc ctcctccccg agaatcgcga gttcatcgtc gcgaaggtgc gcgaggaggg
     1261 agccgacctc gggatcgcct tcgacggcga cgccgaccgc tgcttcttcg tagacgacac
     1321 gggcgagttc gtgcccggcg acttcgtgac cgcgctcctc gccgagctca tgctcgacaa
     1381 ggcgccgggc tccaaggtga tctacgacgt acgcgcgagc tgggcggtcg cccatgccgt
     1441 cgagcgggcg ggcggggtgc ccctcgtgaa tcgcgtcggg cacgcgttca tcaagcaccg
     1501 gatgcgcgag gaggacgcgg tcttcgcggg cgaggtctcg gggcactact acttccgcga
     1561 cttctgccag gccgacaccg gcacggtccc ggcgctcctc ctcctcgagc acgtctcgaa
     1621 ggccggcgtc ccgctctcgg agctccttcg gccctaccgc gagcgctact tcattaccgg
     1681 ggagctgaac acgcgcgttc gggacgtcgc gctgaagctc caggaggtga aggagcggtt
     1741 tggccgcgag gggcgcgtct cccacatcga cggcctcgag gtgaccgcgg acgactggca
     1801 cctgaacctc cgtccctcga aca
//
LOCUS       DASQDO010000424         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_840775, whole genome shotgun sequence.
ACCESSION   DASQDO010000424 DASQDO010000000
VERSION     DASQDO010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840775"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1393
                     /locus_tag="VEV43_11795"
     CDS             77..1393
                     /locus_tag="VEV43_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24247.1"
                     /translation="MRRNHVTLLLVSLLAASVPVPPAAAAPSCFGRAATIVGTDDDDE
                     ITGTPGRDVIYAGKGRDEVHAGGGKDLVCGAGAIDLLYGEGGNDRLQGGPGYVDDWLF
                     GGGGADRLLPGCNGPEGDLMEGGAGPDRLLGNPCGSASMVPGPGDDFVRAIRGEVTFP
                     HASGPIDADLTEGVVTGEGRDVIEVVRRDPGSDDRLVTVVGTDFDDRLVGGPLPDSFQ
                     GRGGDDVIEGRGGADFLDPNTGADTISGGGGDDLVVVYDGTVAAGGPGDDAVLCEPRF
                     ERQGATFDGGAGSDWVSFFGLPGEGVVDLGAGTATCSFTYESSLPPTTVVMELALESV
                     ENVESDRTRLDVTGSAERNVLRGGSGGDVLRGAGGDDEIDGDNGSDEIRGGDGDDRLL
                     GGTWGDELFGDAGDDFLDGEEGPDSVDGGEGRDECSGETRTACEDS"
     gene            1516..3693
                     /locus_tag="VEV43_11800"
     CDS             1516..3693
                     /locus_tag="VEV43_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYP24248.1"
                     /translation="MNARERGGRRRWPGRGGLAITLPALIVTGCVAGAGVMLHANRDG
                     ARSGLERRFRERAVLASEFLGTYVVEQATAEKEAAGDLLAERGVSQAQLTAAVERLGV
                     EAAVVLDAEGTLLAVHPPKPEILGSDLAAEYDHLAGAVAGRTSVSDVVPSAAEGIPIV
                     GIATPYETRHGSRVFSGAFDVSTTPLKPYLESLIPIEDGSALLVDGTGKVVAASEPGR
                     DAIRSWSTGYVEEGGTERYVAAADVAGTNWTLLASAPRAELLQPLEKGDQLAWVLLAG
                     LALTGLLVVVLLVGLRARRRQLERRATECPVTGLANRASIEARIAHALRRGQRTGELL
                     GVLYLDVDDFKVVNDTHGHGVGDELLRVLARRLEDATRATDTPSRIGGDEFAVLLEDI
                     AAPTDAMRVATRVMYELEQPVVIDGVSLNVHASIGIAVSTASGATAASLLRDADAAMY
                     KAKNDGKARIEVFEAELRQQIAAELELEADLRNAIVREQLDLHYQPIVDLASNEVVSM
                     EALLRWHHPVRGDVPPAVFIPIAERNGLIVDIGRFVLRRACAQLGVWSRSAGPVRMCV
                     NVSARQLRDRSLVGDVTEALAAAGVEPGSLTLEITETSLVQDNPVTSESLRRLKELGV
                     TIAVDDFGAGYSSLSYLRRFPIDVLKIDRELVAGITGAAGEAALARAVVQLGQTLGLG
                     IVAEGIESRDQLDALAALGCRTGQGFYFAKPAPAAAVKMGVSV"
     gene            complement(3690..>4830)
                     /locus_tag="VEV43_11805"
     CDS             complement(3690..>4830)
                     /locus_tag="VEV43_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYP24249.1"
                     /translation="FAVAALTFAGWALLRDDPSGGLLAAVAVLIIACPCALGLATPTA
                     IMVGTGRGADLGILIKSADVLERTRSISTVLFDKTGTLTRGSMSLTDVEATGADKDEL
                     LSFAAAVEADSEHPIGAAIAAAGPRDRLPVEGFEAVAGFGVRGRVRGVEVWVGRRKLM
                     AEAGLVIPERLEERACELEDAGKTAVFAGWEGEARGVLAVSDTVKDGAAAAVRSLQAI
                     GLRVVMVTGDNARTAAAVAGELGLDGVLAEVLPQDKVAEVARLQDAGEVVAMVGDGVN
                     DAPALVQSDLGIAIGTGTDVAIEAGDLTIMSGDPRNVELAISLARRTHRTIVQNLFWA
                     FGYNVAAIPLAAMGLLSPVLAGAAMAFSSVSVVTNSLRLRGFRKR"
BASE COUNT          686 a         1748 c         1735 g          661 t
ORIGIN      
        1 caacgcgttg aaggccgtgc aggcgctgcc ctcgggctga gcggaacgat tcacgggggc
       61 cgggcgttct aagcccatga gacgaaacca cgtcaccctc ctgctcgtat cgctgctcgc
      121 ggcgagcgtt cccgtcccgc ccgcggccgc ggcgccgagc tgcttcggcc gagccgccac
      181 gatcgtcggg accgacgacg acgacgagat caccgggacc ccgggtcgcg acgtcatcta
      241 cgcgggcaag ggccgcgacg aggtccatgc cggcggcggc aaagacctcg tctgcggcgc
      301 gggggcgatc gacctcctgt acggcgaggg cggcaacgac cgcctccaag gggggcccgg
      361 ctacgtcgac gactggctct tcggcggggg cggtgccgac cggctgctgc cgggctgcaa
      421 cggcccggag ggagatctga tggagggagg agcggggccg gaccggctcc tcgggaaccc
      481 gtgcgggagc gcgtcgatgg tccccgggcc gggcgacgac ttcgtgcgcg cgatccgcgg
      541 ggaggtcact ttcccgcatg cgtccggccc gatcgacgcg gacctcaccg agggtgtcgt
      601 taccggcgag ggccgggacg tgatcgaggt cgtccggcgc gaccccggct ccgacgaccg
      661 tctcgtcacc gtcgtcggga ccgatttcga cgaccgcctg gtcggcggcc ctctccccga
      721 ttccttccag ggcagaggcg gcgacgacgt gatcgagggc cggggtggag cagacttcct
      781 cgacccgaac accggtgccg acacgatctc cggcgggggc ggcgacgact tggtcgtggt
      841 ctacgacggc acggtcgccg ccggggggcc gggcgacgac gcggtcctct gcgagccgcg
      901 gttcgagcgc cagggcgcga cgttcgacgg gggggcgggc agcgactggg tgtcgttttt
      961 cggtctcccc ggtgagggcg tcgtcgacct cggcgccggc acggcgacct gctcgttcac
     1021 gtacgagtcc tccctcccgc ccacgaccgt cgtgatggag ctggcgctcg agagcgtcga
     1081 gaacgtcgag tcggatcgca cccgtctcga cgtcaccggc tctgcggagc gcaacgtcct
     1141 gcgcggcggc tcgggcggcg acgtcctgcg cggcgccggc ggcgacgacg agatcgacgg
     1201 cgacaacggc tccgacgaga tcaggggtgg cgacggggac gaccgtctgc tcggtgggac
     1261 gtggggcgac gagctcttcg gcgacgcagg cgacgacttc ctggacggcg aggaggggcc
     1321 ggactccgtg gacggcggcg agggccgcga cgagtgctcc ggcgagacaa ggacggcctg
     1381 cgaggactcc tgacggcgtc acacaggaag taactggctc ctcccccggg ctaaagcacg
     1441 ccccccgacg tcccgacgga ccctcatagg ggcggacccg gacgaaggtc cgctaggggc
     1501 cgaaggagag ctgggatgaa cgcacgcgag cgcggcggcc ggcgcaggtg gcccggccgc
     1561 gggggccttg cgatcacgct tccggccctg atcgtcacgg gctgcgtggc gggcgccggc
     1621 gtgatgttgc acgcgaaccg cgacggggcc cgtagcgggc tggagcgccg cttccgcgag
     1681 cgcgccgtcc tcgcgtcgga gttcctgggc acctacgtcg tcgagcaggc cactgcggag
     1741 aaagaggccg ccggcgacct tctcgcagag cgcggcgtct cccaggctca gctcacggcc
     1801 gccgtggagc ggctcggggt cgaggccgcc gtcgtgctgg acgccgaggg cacgttgctc
     1861 gcggtgcacc cgccgaagcc cgagatcctc ggctccgacc tcgccgccga gtacgaccac
     1921 ctggccggag cggtcgcggg gcgtacctcc gtctccgacg tcgtcccctc cgccgccgag
     1981 ggcatcccga tcgtcgggat cgcgaccccc tacgagacgc ggcacgggag ccgcgtgttc
     2041 agcggcgcgt tcgacgtctc gaccacgcct ctgaagccgt acctcgagag cctcatcccg
     2101 atagaggacg ggtccgcgct gctcgtcgac ggcaccggga aggtcgtcgc cgcgagcgag
     2161 ccgggccggg acgcgatccg gtcgtggtcg acgggctacg tagaagaggg cgggaccgag
     2221 cgctacgtcg ccgccgcgga cgtggccggc accaactgga cgctgctcgc gtcggccccg
     2281 cgtgcggagc tgctgcaacc gctcgagaag ggcgaccagc tcgcgtgggt actcctcgcc
     2341 ggcctcgcgc tcacgggcct cctcgtcgtc gtgctgctcg ttgggctgcg cgcccggcgc
     2401 cggcagctcg aacgccgcgc cacggagtgt cccgtcaccg gcctcgcgaa ccgcgcgtcg
     2461 atcgaggccc ggatcgccca cgctctcaga cgggggcaga ggaccggcga gctgctcggc
     2521 gtcctctatc tcgacgtcga cgacttcaag gtcgtgaacg acacgcacgg tcacggcgtc
     2581 ggggacgagc tgctgcgcgt cctggcgcgg cgtctcgagg acgccacgcg cgccaccgac
     2641 acgccgtccc gtatcggagg agacgagttc gccgtgctgc tcgaggacat cgccgcaccg
     2701 accgacgcga tgcgggtcgc gacccgggtc atgtacgagc tcgagcagcc cgtcgtgatc
     2761 gacggcgtgt ccctgaacgt ccacgccagc atcgggatcg cggtctcgac tgcgtccggc
     2821 gcgaccgcag cgtctctgct gcgcgacgcc gacgcggcga tgtacaaggc gaagaacgac
     2881 ggcaaggcgc gcatcgaggt cttcgaggcg gagctgcgcc agcagatcgc ggccgagctc
     2941 gagctcgagg cggaccttcg caacgccatc gtccgcgagc agctggacct ccactaccag
     3001 ccgatcgtcg acctggcctc gaacgaggtg gtctcgatgg aggcgctgct ccggtggcat
     3061 caccccgtgc gcggcgacgt cccaccggcc gtgttcatcc cgatagccga gcgcaacggg
     3121 ctcatcgtcg acatcggaag gttcgtgctc agacgcgctt gcgcgcagct cggcgtgtgg
     3181 agccgttccg cgggaccggt ccggatgtgc gtgaacgtct cggcgcgcca gctccgcgac
     3241 cgcagcctcg tcggcgacgt gacggaggcg ctcgctgccg ccggcgtaga acccgggagc
     3301 ctgacgctgg agatcaccga gacctcgctc gtccaggaca accccgtcac gtcggagtcg
     3361 ctgcgacggc tcaaggagct cggggtcacg atcgccgtcg acgacttcgg cgccggctac
     3421 tcgtcgctgt cgtatctgcg ccgtttcccg atcgacgtgc tgaagatcga ccgcgagctg
     3481 gtcgcaggca tcacgggggc cgcgggcgag gcggcgctgg cgcgcgccgt cgtccagctc
     3541 ggccagacgc tcggcctcgg catcgtcgcc gaagggatcg agagccgcga ccagctcgac
     3601 gcgctggccg ctctcggctg ccgcacgggc cagggcttct acttcgccaa gccggctccc
     3661 gcagcggccg taaagatggg ggtctcggtc tagcgtttgc ggaagccgcg caggcgcagc
     3721 gagttcgtca ccacgctcac gctcgagaag gccatcgccg cgccggcgag caccgggctc
     3781 agcaatccca tggccgccag cggtatggcg gccacgttgt agccgaacgc ccagaagagg
     3841 ttctgcacga tcgttcggtg cgtgcggcgg gcgagcgata tcgcgagctc gacgttgcgg
     3901 gggtcgccgc tcatgatcgt gaggtctccg gcctcgatcg ccacgtccgt gcccgtcccg
     3961 atcgcgatcc cgaggtccga ctgcaccagg gccggcgcgt cgttgacccc gtcccccacc
     4021 atcgcgacga cctcgcccgc gtcctggagg cgcgcgacct ccgcgacctt gtcctgaggg
     4081 aggacctcgg ctaggactcc gtcgagaccg agctcgccgg cgaccgcggc cgcggtgcgt
     4141 gcgttgtccc cggtcaccat gacgacccgc agcccgatcg cttgcagcga gcgcacggcg
     4201 gcagcggctc cgtccttcac ggtgtcggac accgcgagca ctcccctggc ctcgccttcc
     4261 caacccgcga acacggcggt cttcccggcg tcctccagct cgcacgcgcg ttcctcgaga
     4321 cgctcgggga tgaccaggcc cgcttcggcc atgagcttgc ggcgcccgac ccacacctcg
     4381 acaccccgga cgcgcccgcg caccccgaag ccggcgaccg cctcgaagcc ctcgaccggc
     4441 agccggtcgc gcggtcccgc ggccgcgatc gccgctccga tcggatgctc gctgtcggct
     4501 tcgaccgccg cggcgaagct cagcagctcg tccttgtcgg cgcccgtcgc ttcgacgtcc
     4561 gtcaacgaca tcgaaccccg cgtcagcgtc cccgttttgt cgaacagcac cgtcgagatc
     4621 gatcgcgtcc gctcgagcac gtccgcgctc ttgatcagga tcccgaggtc cgcgccgcgc
     4681 ccggtcccga ccatgatcgc ggtaggcgtc gcgaggccca gagcgcaggg gcaggcgatg
     4741 atgagcacgg cgaccgcggc gagcaacccg ccggaggggt cgtcccgcaa gagcgcccag
     4801 cccgcgaacg tcagcgcggc cactgcgaag
//
LOCUS       DASQDO010000425         7221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1086092, whole genome shotgun sequence.
ACCESSION   DASQDO010000425 DASQDO010000000
VERSION     DASQDO010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086092"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1282)
                     /locus_tag="VEV43_11810"
                     /pseudo
     CDS             complement(1..1282)
                     /locus_tag="VEV43_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(1351..2136)
                     /locus_tag="VEV43_11815"
     CDS             complement(1351..2136)
                     /locus_tag="VEV43_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYP24250.1"
                     /translation="MSCRYDVSNGVAVVTLDRPDVLNAFDDELGFALLDAVRRASDDD
                     AVRCIVLTGAGRAFSSGEDLGALQADYERGEAPPLGNTLIERYNPLIRALRAAPKPVL
                     AAVNGVAAGAGASIALACDFRIASDKAKLVLAFIKAGLVPDSGAVWFLLRTCGASRAW
                     YLAATGDPMSAEEAEACGIFHRVVPVDEFEGAWRAWAEELAAGPTRAYALTKQLVNHA
                     ASASLDDQLDLEVDAQTQAGATADHLEGVRAFLGKRRPDFKGR"
     gene            2187..2507
                     /locus_tag="VEV43_11820"
     CDS             2187..2507
                     /locus_tag="VEV43_11820"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HYP24251.1"
                     /translation="MNLGPIEIFFFVGILLALAPGFVGLALAPSANVPGIVGFLIGLF
                     GSWLGVIGLYLYGHSQKQKAPAVEIGRPAPADRLRALQKLLDEGLITQEEYEARRRAA
                     MDDL"
     gene            2508..3344
                     /locus_tag="VEV43_11825"
     CDS             2508..3344
                     /locus_tag="VEV43_11825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP24252.1"
                     /translation="MADARDESRDTWDRMAPGWERHRDLMWATTRHVAEWLVGRVEPQ
                     PGHVILDLAGGVGDNGFLAAPLVGDTGRVIVTDFAPQMVETARRRAEQLGLGNVETRV
                     LDAERMDLDDDSVDGIVCRWGFMLMLDPQSALKECRRVLKDGGRLALSVWAGPEKNPW
                     VTTTGMTMMQLGHEPAHDPFGPGGIFSLADHDALRSLLADAGFSDVTIEEMPVDWKYE
                     SFAQSWEFTSEVAGALASLVSELPPDQVEELRAALEKNEEPFRTHEGLVLPGTTVNAA
                     AS"
     gene            complement(3341..3766)
                     /locus_tag="VEV43_11830"
     CDS             complement(3341..3766)
                     /locus_tag="VEV43_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP24253.1"
                     /translation="MGKLKVLVADDEPALRKLLKTNMELEGYETLEASNGAEVLQCVE
                     RDNPDIILLDIMMPVMDGWEVMTALAANPEYEQKVILVSAKASDDAQLQGWELGADEY
                     ITKPFDLESLLQRVRDVAARTEAESEARRAEAISELQNL"
     gene            complement(3809..4579)
                     /locus_tag="VEV43_11835"
     CDS             complement(3809..4579)
                     /locus_tag="VEV43_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP24254.1"
                     /translation="MATGDRLTELEAALAERDARIAELEELDKHKNDFVATVSHELRT
                     PITAMIGAAKTLLRGGDQMSAEERASFLEMIERQGDRLLRLTRGVLAGAQVESGAALR
                     REKVDLGALARDVADELLSTGRSTHEVEVNAEPEGASAWGDPTALRQIMSNLVENALK
                     YSPEGSKVRVGVLDLSSETLLEVSDEGPGIDAGSVGTIFERFKQMESSRSGGFGLGLY
                     IVKNLVEQHRGTIEVSGEAGAGTTFRVRLPKRAARRER"
     gene            complement(4558..6012)
                     /gene="purF"
                     /locus_tag="VEV43_11840"
     CDS             complement(4558..6012)
                     /gene="purF"
                     /locus_tag="VEV43_11840"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYP24255.1"
                     /translation="MSGEARDACGVVGIYAPGEDVARLTYYGLYALQHRGQEAAGIAV
                     SDGQTILVYKELGLVSQVFDETVLNSLRGTLAIGHVRYSTTGSTSWENAQPSFKSSPH
                     GQVALAHNGNLVNSVALKRRLEEAGGDPTPEAARLPSSSDTELVAAHIARANTGDMRR
                     AILDVLPGLSGAFSFTIMDERRVYGARDPWGFRPLSIGRLEDGWVLASETCALDLLGA
                     RFIRDVEPGELVTIGPEGLESERFAPARYATCVFEHVYFARPDSTLMGQNVYATRYRM
                     GQRLAQEAPVTGELVMPVPDSGVPAAQGFAQASGIPYGDGFVKNRYVGRTFIQPTQSM
                     RQQGIRTKLNPVREVIEGKRVVVLDDSIVRGNTTRQIVAMLRDAGAREVHMRVSSPPI
                     KWPCFYGIDMPDQDDLIGSRMSVDEIGAHIKADSLAYLSIDGMLTSTGIPSDDFCTAC
                     FSSRYPVPIPSEELRSKHVLEQPDPATAWQPETG"
     gene            complement(6009..>7221)
                     /locus_tag="VEV43_11845"
     CDS             complement(6009..>7221)
                     /locus_tag="VEV43_11845"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HYP24256.1"
                     /translation="RRWGLEASVLGEVSKDGRLKVLAGGETVVDAPAEALAEEGPVYQ
                     RPFERPAWLDEVQAAPVGDTAPPDPRTAFLQLLSSPGIASKRWVWEQYDHMIFLGTLH
                     GPGSDAAVIRLPGTDVAVAITVDGPGRLCYLDPYEGALHAVAEAARNVACSGAKPLAV
                     TNCLNFGNPEKPDVMWQFAEVVRGIGDACRALATPVTGGNVSFYNETMGRAIYPTPVI
                     GMAGVCHSPEQAVGFRFRADGDVLLLLGSPDPDAFGGSEYAKVVNVAIGGKPPALDLV
                     AERRLHDLLFEAAGAGVLRSAHDVSSGGLAVTLAESAIAGGKGFELSAFEGEPHRALF
                     AEPASCVVVTTDDEDALRALADGAGVPVTRLGIVGGDALRFGSFDVSLEEATAAFETA
                     LPERLSATIDA"
BASE COUNT         1062 a         2494 c         2555 g         1110 t
ORIGIN      
        1 cgggggcgtc gctgagcggg aggatcgcca gctccgcgag gtcgccggcc actgctgcca
       61 tgggggccgg actccgttcg atcccgagag cggccgacgt cgccgagcgg acgcgttccc
      121 agccttcttc cagggacgcg tccgagggct tgagcgcggg ggcgtccggg agcgacttca
      181 gcttgcccgc cctctggccc gcgatcgcac gcgcggcccg gcggccgaag accaacgcct
      241 ccgccagcga gttcccggcg agccggttcg ctccgtgcac gccggtgttg gagcactcgc
      301 cgaccgcgta cagacgcggc acggacgaac gaccgtcgag gtcggtctgg acgcctccga
      361 tgaagtagtg cgcggcgggc gcgaccggga tgaggtcggt gcgcgggtcg atgccggact
      421 ctctgcacgc ggccatcacc gtgaagaact cgctctcgag cagctccgga tcgagatggc
      481 ggcagtcgag ccacgagtga cgcgacgcga tcgcgcgtgc gacgacgtcg cgcggcgcga
      541 gctctccgtc ggagtggtgc tcgaagagga accgccggcc ttcggagtcg acgacgtgag
      601 ctcccgctcc gcggagcgct tcggtgatca acggacgctg ctgccggtcg tcgagcgcga
      661 gcgcggtcgg atggaactgg acgaactcga cgtcggcgag cccggccccc gcctcccagg
      721 ccagcacgag accgtcaccg gtcgcacccg ccgggttggt cgtcgcgcgg aacagcccgc
      781 cgaccccgcc cgtggcgagg acggtcgacg tcgcgccgac ctcgacgatg ccttcgtcgg
      841 tgaggatcca cgcgcctgcg caacgaccgt cgacgactgc gagcttgaga cacgtcccct
      901 cgatgcggcg aacacgggga gtcgccttct cgcgcagggt cttcatgatc gccgccccgg
      961 tcgcgtcgcc cgcgtgcgcg gagcgcgcta ccgactgtcc gccttcgcgg gcgagatgca
     1021 gctcgccttc gggagtgcgg tcgaagtcac tcccacgctc ggcgagctgg cggattcacg
     1081 aggggggctc gtagccgaga accgcgacgg cagacgggtc cgcgtgtccc gcggccacgc
     1141 gcatcgtgtc ctgcgcgtgg aggtcgggcg agtcctcggg gccgagcgcg gctgcgacgc
     1201 caccctgtgc ccacggcgac gagccctgtc ccttgcggcc cttgtcgacg acgaggacgt
     1261 ccgttccctc ggggaggtgc atcgacgcga acagcccggc cgcgcccgcg ccgatgacga
     1321 gcacgtcggt cttgagcttc gggatcgcca ctagcggcct ttgaagtccg gtcgtcgctt
     1381 gccgaggaac gcgcgcactc cctcgaggtg gtcggcggtc gcgcccgcct gcgtctgcgc
     1441 gtcgacctcg aggtcgagct ggtcgtcgag gctcgccgac gccgcgtggt tcacgagctg
     1501 cttcgtgagg gcgtaggccc gggtggggcc ggccgccagc tcttccgccc aggcgcgcca
     1561 tgcgccctcg aactcgtcga ccgggacgac gcggtggaag atgccgcacg cctccgcttc
     1621 ctctgcggac atcggatcgc cggtcgcggc gaggtaccag gcgcggctcg cgccacacgt
     1681 ccgcaggagg aaccagaccg cgccggagtc gggcacgagt cccgccttga tgaacgcgag
     1741 gacgagcttc gccttgtcgg acgcgatgcg gaagtcgcat gcgagcgcaa tgctcgcgcc
     1801 cgcccccgcg gcgacgccgt tgaccgcggc gagcacgggc ttgggcgcgg cgcggagagc
     1861 gcggatcaac gggttgtagc gctcgatcag cgtgttgccc agcggggggg cctctccgcg
     1921 ttcgtagtcg gcctggaggg cgccgaggtc ctcgccggac gagaacgccc gcccggcccc
     1981 cgtgaggacg atgcagcgga cggcgtcgtc gtcggaggcg cggcggacgg cgtcgaggag
     2041 ggcgaacccg agctcgtcgt cgaaggcgtt gaggacgtcg ggccggtcga gggtgacgac
     2101 ggcgacgccg ttggagacgt cgtagcgaca agacatggcc cgaagcgtaa ctcggcccat
     2161 aatgggcgcg gctaggaggt gagcgaatga acttggggcc tatcgagatc ttcttcttcg
     2221 tcgggatcct gcttgcgctc gcgcccggct tcgtcggcct tgcactggct ccgtcggcca
     2281 acgtccccgg gatcgtgggc ttcctgatcg ggttgttcgg gagctggctc ggcgtgatcg
     2341 ggctttactt gtacggacac tcgcagaagc agaaggcccc ggcggtcgag atcggcagac
     2401 ccgccccggc cgaccgtctg cgggcgctgc agaagctcct cgacgaggga ttgatcacgc
     2461 aggaggagta cgaagcgcgc cgccgggcgg cgatggacga cctctaggtg gccgacgccc
     2521 gcgacgaaag ccgcgacacc tgggaccgga tggctcctgg gtgggagcgc catcgcgacc
     2581 tcatgtgggc cactacccgc cacgtcgccg agtggctcgt cgggcgggtc gagccgcagc
     2641 cgggacacgt gatcctcgac ctcgccggcg gagtcggtga caacgggttc ctcgcggccc
     2701 ccctggtcgg cgacaccgga cgcgtgatcg tcacggactt cgcaccgcag atggtcgaga
     2761 ccgcgcgcag gcgggccgag cagctcggtc tcggcaacgt cgagacgcgc gtcctcgacg
     2821 ccgagcggat ggacctcgac gacgactccg tcgacgggat cgtctgccgc tggggcttca
     2881 tgctcatgct cgacccgcag tccgcgttga aggaatgccg tcgtgtgctc aaggacggtg
     2941 gccgtctcgc gctcagcgtc tgggcgggcc ccgagaagaa tccgtgggtc acgacgaccg
     3001 ggatgacgat gatgcagctc ggtcacgagc cggcgcacga ccccttcggg ccgggaggga
     3061 tattctcgct cgccgaccac gacgcgctgc gctccctcct cgccgacgcc ggcttctccg
     3121 acgtcacgat cgaagagatg ccggtggact ggaagtacga gtcgttcgcg cagtcatggg
     3181 agttcacgag cgaggtggcc ggggctctcg cgtcgctcgt cagcgagctg ccgccggacc
     3241 aggtcgaaga gctacgcgcg gccctcgaga agaacgaaga gccgttccgg acgcacgagg
     3301 gcctggtcct gccgggcacg accgtgaacg ccgcagcttc ttagaggttc tgcagctccg
     3361 agatcgcttc ggcccttcgg gcttcagact cggcttccgt gcgcgccgct acgtccctta
     3421 ctcgctgcag tagcgactcg aggtcgaacg gcttcgtgat gtattcgtcg gcccccagct
     3481 cccagccttg gagctgcgcg tcgtcggacg ctttcgccga gaccaggatc accttctgct
     3541 catattccgg gttcgcggcc agcgcggtca tcacttccca gccgtccatg accggcatca
     3601 tgatgtcgag caggatgatg tcggggttgt cgcgctcgac gcactgaagc acctcggcgc
     3661 cgttcgacgc ctcgagcgtc tcgtagccct cgagctccat gttcgtcttc aggagcttgc
     3721 ggagcgcggg ctcgtcgtcc gctaccagga ccttcagctt ccccaaccga gctccatcct
     3781 tccgccggca tgccggaccg tgaaaagcct accgctcccg tcgcgccgcc cgtttgggca
     3841 gccggacccg gaaggtcgtc ccggcccccg cttcgcccga cacctcgatc gtgccgcggt
     3901 gctgctcgac caggttcttc acgatgtaga gaccgaggcc gaagcctccg ctgcgcgacg
     3961 actccatctg cttgaacctc tcgaagatcg tcccgacgga gccggcgtcg atgcccggac
     4021 cttcgtcgga cacttcgagg agggtctcgg aagaaaggtc caagacccca actcttacct
     4081 tcgacccctc cggcgaatac ttgagtgcgt tctcgacgag gttcgacatg atctgccgga
     4141 gcgcggtcgg gtcgccccac gccgacgcgc cctcgggctc ggcgttcacc tcgacctcgt
     4201 gcgtcgaacg ccccgtcgac agcagctcgt cggcgacgtc gcgggccagc gcgcccaggt
     4261 cgaccttctc gcgccggagg gccgcgcccg actcgacctg cgcgccggcg aggacaccgc
     4321 gcgtgagacg aaggagacga tcgccctgac gctcgatcat ctcgaggaac gacgcgcgct
     4381 cctcggccga catctggtcg ccgccgcgca gcagcgtctt ggcggcgccg atcatcgcgg
     4441 tgatgggggt ccggagctcg tgcgacaccg tcgcgacgaa gtcgttctta tgcttgtcga
     4501 gctcctcgag ttcggcgatg cgagcgtccc gctcggccag cgccgcttcg agctcggtca
     4561 gccggtctcc ggttgccatg ccgtcgcggg atcgggttgc tcgagcacgt gcttgctgcg
     4621 gagctcctcg ctcgggatcg ggaccggata gcgcgacgag aaacaggcgg tgcagaagtc
     4681 gtcggacggg atgccggtcg acgtgagcat cccgtcgatc gacaggtatg ccagcgagtc
     4741 ggccttgatg tgcgcgccga tctcgtcgac ggacatgcgc gagccgatga ggtcgtcctg
     4801 gtcgggcatg tcgatcccgt agaagcacgg ccacttgatc ggcggggacg agacgcgcat
     4861 gtggacctcg cgcgcgccgg cgtcccgcag catcgcgacg atctgacgcg tcgtgttgcc
     4921 gcgcacgatc gagtcgtcga ggacgaccac gcgcttgccc tcgatgacct cgcggacggg
     4981 gttgagcttc gtgcggatcc cctgctggcg catcgactgc gtcggctgga tgaacgtgcg
     5041 gccgacgtag cggttcttca cgaagccgtc gccgtagggg atccccgacg cctgcgcgaa
     5101 gccctgcgcg gcgggcacgc cgctgtcggg caccggcatg acgagctcgc ctgtgacggg
     5161 ggcctcctgc gcgaggcgct ggcccatccg gtagcgggtg gcgtagacgt tctggcccat
     5221 cagcgtcgag tcggggcggg cgaagtacac gtgctcgaac acgcacgtcg cgtagcgcgc
     5281 cggcgcgaag cgctcgctct cgaggccttc cggcccgatc gtgacgagct cgccgggctc
     5341 gacgtcgcgg atgaaacggg cgccgagcag gtcgagggcg caggtctcgg acgcgagcac
     5401 ccatccgtct tcgagacggc cgatcgacaa cggccggaac ccccacgggt cgcgggcgcc
     5461 gtacacgcgt cgctcgtcca tgatcgtgaa cgagaacgcg cccgagagcc ccggcagcac
     5521 gtcgaggatc gcccgtcgca tgtctccggt gttggcgcgt gcgatgtgcg cggcgacgag
     5581 ctcagtgtcg gacgaggacg ggagtcgcgc ggcctccggc gtcgggtcgc ctccggcttc
     5641 ttcgagccgg cgcttcagcg ccaccgagtt cacgaggttg ccgttgtgcg cgagcgcgac
     5701 ctggccgtga ggcgacgact tgaacgacgg ctgcgcgttc tcccacgagg tggagccggt
     5761 cgtggagtaa cggacgtggc cgatcgccag cgtcccgcgc aggctgttca gcaccgtctc
     5821 gtcgaacacc tgcgagacga ggccgagctc cttgtagacg aggatcgtct ggccgtccga
     5881 gaccgcgatg cccgcggcct cctgcccccg gtgctgaagg gcgtagagcc cgtagtaggt
     5941 cagccgggcg acgtcctcac cgggggcgta gatgccgacg acgccgcagg cgtcacgagc
     6001 ttccccactc atgcgtcgat ggtagccgag aggcgctcgg gcagggcggt ttcgaatgcg
     6061 gcggttgctt cttcgagcga tacgtcgaac gagccgaagc gcaaggcgtc gccgcctacg
     6121 atgccgagcc gggtcaccgg gacgccggct ccgtccgcca gcgcacgcag cgcgtcttcg
     6181 tcgtcggtgg tgacgaccac gcacgacgcg ggctcggcga acagtgccct gtggggctct
     6241 ccctcgaatg cgctcagctc gaaccccttc cctcctgcaa tcgccgactc ggcgagcgtc
     6301 acggcaagcc cccccgatga aacgtcgtgt gcggaccgaa gcacacctgc gccggccgcc
     6361 tcgaagagga gatcgtgcag acgtctctct gcaacgagat cgagggccgg tggcttccca
     6421 ccgatcgcga cgtttacgac ctttgcgtac tcgctgccgc cgaaggcatc ggggtcgggc
     6481 gaaccgagca gtagaaggac gtcaccgtcg gcgcggaacc ggaaccccac cgcctgctcc
     6541 ggtgagtggc acacgcccgc catgccgatc accggcgtcg ggtagatcgc gcggcccatc
     6601 gtctcgttgt agaaggacac gttcccgccg gtgaccggcg tcgcgagggc ccggcaggcg
     6661 tcgccgatgc cgcggacgac ctcggcgaac tgccacatca cgtcgggctt ctcggggttc
     6721 ccgaagttga ggcagttcgt caccgcgagc ggcttcgcac cggagcaagc gacgttgcgc
     6781 gccgcctcgg cgaccgcgtg gagcgcgccc tcgtaggggt cgaggtagca gaggcggccg
     6841 ggcccgtcga ccgtgatcgc gacggcgacg tccgttcccg gcagccggat gaccgcggcg
     6901 tcggagccgg ggccgtgcag cgtgccgagg aagatcatgt ggtcgtactg ctcccagacc
     6961 catcgcttcg acgcgatgcc gggagacgac aacagctgga ggaacgcggt gcgcgggtct
     7021 gggggggccg tgtcgccgac gggcgcggcc tgcacttcgt ccagccatgc cgggcgctcg
     7081 aacgggcgct ggtagaccgg gccttcttcc gccagcgcct cggccggcgc gtcgacgacg
     7141 gtctcgccgc cggcgagcac cttgaggcgg ccgtccttcg acacctcgcc cagcaccgaa
     7201 gcctcgagtc cccagcggcg g
//
LOCUS       DASQDO010000426         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_596091, whole genome shotgun sequence.
ACCESSION   DASQDO010000426 DASQDO010000000
VERSION     DASQDO010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596091"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VEV43_11850"
     CDS             complement(<1..331)
                     /locus_tag="VEV43_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24257.1"
                     /translation="MSRRAIAALVVSLLAANAVAFPPGAAANFPGANGKIAFVRGEAG
                     SETSQIFVAGPEGSDPRRITSTPRHYGGLDWSADGSKLAFEVATTDGTEIWVMNHDGT
                     EPRRVSVA"
     gene            403..1122
                     /locus_tag="VEV43_11855"
     CDS             403..1122
                     /locus_tag="VEV43_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HYP24258.1"
                     /translation="MTIDYTAHLRADGERMTLAVGRDLDAHVPTCPAWNVAKLTIHTG
                     QHHRWVADAVRKRGEMPEDPPKPGLRGDELVEWFREGWRDLADLLDTVGEDDPAWSWS
                     GDNRAGFWKRRTALETLVHRWDAENAVGSAGALDPELAADGIDEALFVMTGEGGAYDG
                     VAFRMVVTTSDAGGPWTLELVDGRLPRASRELATGDDVVTVSGTAGDVLLFLWGRHGA
                     EAVALDGSEEAKAALLRWTDE"
     gene            1119..1835
                     /locus_tag="VEV43_11860"
     CDS             1119..1835
                     /locus_tag="VEV43_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HYP24259.1"
                     /translation="MNDAELSGRLSEVREAEARLAAMVAGLDDAALGEPSLLPGWTRG
                     HVLAHVALNAHSLVNLFTWARTGVETPQYPSWEERDAAIEEHSKRTLAQHRDALAGAG
                     GAFQDAAKAVPLDRWSFQVSGIGGSPQPAETFLFGRLREVEIHHVDLTAGYAPADWPT
                     GFVGRVLAETRARLSSGASTPFAAVATDLDVRIDIGDAAAEVEVAGAGHELLAWLLGR
                     SAGTTLEVRGGTLPEVPTWG"
     gene            1826..2194
                     /locus_tag="VEV43_11865"
     CDS             1826..2194
                     /locus_tag="VEV43_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24260.1"
                     /translation="MGLKPALVAVAVMLAAPACSSSDVAVAPGCLDETLSSYREVVSR
                     AAEGRGNEFRSAARDAADVTAACAKEAVDECRDALGDLASALRDAATDVARSPSSFRE
                     HRTNLEGAAAAATACAREVL"
BASE COUNT          526 a         1050 c         1103 g          442 t
ORIGIN      
        1 ctgcgacgct gacccgtcgc ggctcagtcc cgtcgtggtt catcacccag atctcggtcc
       61 cgtccgtcgt cgcgacctcg aacgcgagct tcgacccgtc cgcggaccag tcgaggcccc
      121 cgtaatgcct gggcgtggag gtgatcctcc gcgggtcgct gccctcggga cccgcgacga
      181 agatctgcga cgtctcgctc ccagcctcgc cacgcacgaa ggcgatcttc ccgttggcac
      241 ccgggaagtt ggcggctgcc ccgggaggga aggcgacggc gttcgcggcc aggaggctca
      301 cgacgagggc ggcgatcgct ctgcggctca tgtcatccga gcgtccgtgg ccgtccgcgc
      361 ccggtcaata gccccttcgg accaagtccg tacgatgggc gtgtgacgat cgactacacc
      421 gcccacctgc gcgccgacgg cgagaggatg accctcgccg tggggcgcga tctcgacgcg
      481 cacgtcccga cctgtcccgc gtggaacgtg gcgaagctca cgatccacac cgggcagcac
      541 caccgctggg tcgccgacgc cgtgcgcaag cgcggggaga tgccggaaga ccctcccaag
      601 cccggcctgc gcggtgacga gctcgtcgaa tggttccgcg agggatggcg tgacctcgcc
      661 gacctgctcg acaccgtcgg cgaggacgac ccggcgtgga gctggtcggg cgacaaccgg
      721 gcggggttct ggaaacggcg gaccgcgctc gagactctcg ttcaccgctg ggacgcggag
      781 aacgcagtcg gttcggcggg cgccctcgat cccgagctgg ccgccgacgg gatagacgag
      841 gcgctgttcg taatgaccgg cgagggtggc gcttacgacg gcgtcgcgtt ccggatggtc
      901 gtcacgacgt cggacgccgg ggggccgtgg acgctcgagc tcgtcgacgg gcggctgccg
      961 cgggcttcac gcgagctcgc gaccggcgac gacgtggtca ccgtgtcggg gacggccggc
     1021 gacgtgctgc tgttcctgtg gggccgtcac ggagcagaag ccgtcgcgct ggacggatcc
     1081 gaggaagcga aggccgcgct cctgcgctgg acggacgagt gaacgacgcg gagctctcgg
     1141 ggcggctgtc cgaggtgcgg gaagcggagg cccgtctggc ggcaatggtc gcggggctgg
     1201 acgacgcggc cctcggagag ccgtcgctgt tgccgggctg gacgaggggc cacgtcctcg
     1261 cgcacgtcgc gctgaacgcg cactctctcg tgaacctctt cacgtgggct cgcaccggcg
     1321 tcgagacgcc ccagtacccg agctgggagg aacgcgacgc ggcgatcgag gaacactcga
     1381 agaggacgct cgcgcagcat cgcgacgcgc tggcgggagc gggcggcgcg ttccaggacg
     1441 ccgcgaaggc cgtgccgctc gaccgatggt cgttccaggt gtcggggatc ggcgggagcc
     1501 cgcagccggc ggagaccttc ctgttcgggc gcctccgtga ggtcgagatc catcacgtcg
     1561 acctgaccgc gggatacgcc ccggccgact ggcccaccgg cttcgtcggc cgggtcctag
     1621 cggagacccg tgcgcggctc tcgtccggcg cgagcacccc gttcgccgcg gtggcgaccg
     1681 acctggacgt gcgcatcgac atcggcgacg ccgctgcgga ggtcgaggtg gcgggggccg
     1741 ggcacgagct gctcgcgtgg ctgctgggcc gaagcgccgg aaccacgctc gaggtacgcg
     1801 gtggaacgct gcccgaggtg ccgacgtggg gctgaagcct gcactcgtcg cagtcgccgt
     1861 catgctggcc gcaccggcgt gctcgtcgag cgacgtcgcg gtggctcccg gctgcctcga
     1921 cgagacattg tcgtcctacc gggaggtagt cagccgcgcg gcggagggcc gcggaaacga
     1981 gttccgctcg gcagcgcgcg acgccgcaga cgtcacggcc gcatgcgcga aggaagccgt
     2041 agacgagtgc cgtgacgcgc taggagacct ggcctcggcg ctgcgcgacg cagcgacgga
     2101 cgtagctcgc tccccgagct cgtttcggga acaccggacg aacctagaag gcgcggccgc
     2161 cgcagccacc gcctgcgccc gcgaggtgct gtagcccgct cacctgccga tcggacggat
     2221 acagccttcg gcgcgcaggc gtgagaggcc cgtcggtcag caggacctac acgcagctca
     2281 cgccgttccc cggaggccgg ggaggcgtcc cggcaaccga ccgcgaagag tagccgcgta
     2341 cgtgatgtag aagcccgcgg caaatccttg atgccggcca acgcgattcc cgacgtccgg
     2401 cggaacgtga acgaaccaac gaacatgagc agcggagaaa tcttcaagaa aagagctccg
     2461 ttgcgagcgc tcgacttgag ctgcatcgcg agcaccagtc gattttcgac ccgccggcgg
     2521 cccccgcaca gcgccaagag gaacgcgctg gacaagaccg ccacacctag gaacaattcg
     2581 cgagaccggt cgaaaagcgc gacgaccgcg accaccaaat acatccgcac cgtaaacgcg
     2641 ctaattgtgg cgagcggggt cccacttcgt cagtgggtcg tcaccacgta aacgaagcgg
     2701 gtggtgctga tcgctccata aggcggcccg tggaagtgtc ctgcgtgatg aagcggcctg
     2761 gctccggcaa gtagtagcgc gttccgtatc cactaccact gttcacgtgg ggtcatccct
     2821 tggttggcaa tgatcgactc gagcgagaat gccgtcgacg aaaatcgtag atcgcatagg
     2881 taacgctgac agcggtggcc gcgacccctg cagcgaacac cgagactcga tactcgggag
     2941 ttcggacgac tagacccgac gcgtacgcga gtaacagtat ccacgtgagt cgatcaagcc
     3001 agcgggtgtt cccccggacg gcgcgcctgc acctctcatt cgaggtaccg cccaatccct
     3061 gtcaaaaggg tcgcgccgag tgcactatcc aggagtaggc gatagaccca cgttggacct
     3121 c
//
LOCUS       DASQDO010000427         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_963300, whole genome shotgun sequence.
ACCESSION   DASQDO010000427 DASQDO010000000
VERSION     DASQDO010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963300"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="mreC"
                     /locus_tag="VEV43_11870"
     CDS             <1..408
                     /gene="mreC"
                     /locus_tag="VEV43_11870"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYP24261.1"
                     /translation="MAVIAPDGLVGKVYSVGAHQATILYLVDPRGAASARVEGGRDIG
                     IVEGRGAGEPLTFDLVSVNAEADEGDRVITSGYDNGIFPAGIPIGFIVDVGGDARQAT
                     KEIDVEPYVDFNTLDFVQVLVESGPMLTQNEGS"
     gene            412..969
                     /gene="mreD"
                     /locus_tag="VEV43_11875"
     CDS             412..969
                     /gene="mreD"
                     /locus_tag="VEV43_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYP24262.1"
                     /translation="MATSVPRGPTILGEVGIPRMLAVGAVLVVALALQSTLLAQATIL
                     GVIPQVVLVVVVCLAYLDGERVGLVTGFSAGLLQDLMPFPAVIGLTALVYTLVGYGVG
                     ILRQYAPTDSVWTPVLVVALASAVAEFSYAGLAIILGEPWVGLAYTARVAGLVVLYNT
                     LLTPFVFPLVRRVADRFRPERVHRW"
     gene            963..3005
                     /gene="mrdA"
                     /locus_tag="VEV43_11880"
     CDS             963..3005
                     /gene="mrdA"
                     /locus_tag="VEV43_11880"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYP24263.1"
                     /translation="MVTNGGPSLGPERGTGDRTVLRLAIMAVLLVAAFVALFSRLWYL
                     QVLAVGDYRTLAKENRIRLVYSEPTRGRILDRDGDVLVGNRQSLSITIDRQRVEPGSL
                     QERRTLYDVARLLVPEDKAKREYKKALVAKVKEMRDNLNDATVSPYKPVAVVNDVREA
                     IVRQIRENREDFPGVEAEKLPIRTYPNGKLAAHVLGYVGEINADELEAPEFQDAKPPY
                     EPGDLIGKSGVERTYDRWLRGRPEIKRVVVNSAGEVVDQAIRQEEEPGRDLILSIDVE
                     AQKLAERSLESGIMAARGAAHPAPDGAIVVMDPNTGGIVAMASYPTYDPSILADGITE
                     REYERMSGSPDTAEDDVLPNRATQAQVPPGSTFKVVTAGAALANDVVTASDYLPCPGQ
                     VSYGATGITTVFNNWTSVDFGSIGFPESLEISCDTFYSELGWRMEELWGAANGDESER
                     FQDYMRHAGFGRPTGIDLPHEEEGRVPDEKWCEEFAKQGLGCFDGWLPGFTVNMAIGQ
                     GDLTTNPLQMAVTYAALAADSGEVWEPHVAQQIGRPDAETGEEEILREIEPEALRRLP
                     LDSTEIGVIRQGLEDVVSGANGTAAGAFAGFPLDQYPVAGKTGTAQIGESEADVNYAW
                     FVSYAPADDPQYVVSVYLAKAGHGGETAAPVAREVFEGLFGIDRETGVTLGQDASG"
BASE COUNT          474 a         1043 c         1065 g          454 t
ORIGIN      
        1 atggccgtga tcgcgcccga cggtctcgtc ggcaaggtct actccgtcgg cgcgcaccag
       61 gcgacgatcc tctacctggt cgacccgcgc ggagctgcga gcgcgcgcgt cgaagggggc
      121 cgcgacatcg gcatcgtcga gggccgcggt gcgggcgagc cgcttacgtt cgacctcgtg
      181 agcgtcaacg cggaagcgga cgaaggtgac agggtcatca cctccggcta cgacaacggg
      241 atcttcccgg cgggcatccc gatcgggttc atcgtcgacg tcggcggcga cgcgcgccag
      301 gcgacgaagg agatcgacgt cgagccgtac gtcgacttca acacgctcga cttcgtccag
      361 gtgctcgtcg agagcggccc gatgctgacg cagaacgagg ggtcgtgacg tatggcgacg
      421 agcgtgccta gagggccgac gatcctcggc gaggtcggga tcccccggat gctcgctgtc
      481 ggcgcggtgc tcgtcgtcgc gctggcgctc cagtcgacgc tgcttgcgca ggcgacgatc
      541 ctcggcgtca tcccgcaggt cgtcctcgtc gtcgtcgtgt gtcttgccta cctcgacggc
      601 gaacgggtcg gtctcgtcac cgggttcagc gcgggcctcc tccaggacct catgccgttc
      661 cccgcggtga tcggcctgac cgccctcgtc tacacgctcg tcgggtacgg cgtcgggatc
      721 ctgaggcagt acgcgccgac ggactccgtg tggacgcctg tgctcgtcgt cgcgctcgcg
      781 tctgcggtcg ccgagttcag ctacgccggt ctggcgatca tcctcggaga gccgtgggtg
      841 gggctcgcct acaccgcccg cgtcgccggg ctcgtcgttc tctacaacac cttgctgaca
      901 ccgttcgtct tcccgctcgt ccgccgcgtc gcggatcgct tccgtcccga gagggtccac
      961 agatggtgac caacggcggg ccgtctctgg gcccggagcg cgggacgggg gaccgcacgg
     1021 tcctgcgcct cgcgatcatg gcagtgctgc tcgtcgccgc gttcgtagcg ctgttctcgc
     1081 gactctggta cctgcaggtc ctcgctgtcg gcgactaccg gacgctcgcg aaggagaacc
     1141 ggatccggct cgtgtactcc gagccgacgc gcggccgcat cctcgaccgc gacggcgacg
     1201 tcctcgtcgg caaccgccag tcgctgtcga tcacgatcga ccgtcagcgc gtcgagccgg
     1261 gttcgctcca agagcggaga acgctctacg acgtcgcacg cctcctcgtc cccgaggaca
     1321 aggcgaagcg cgagtacaag aaggcgctgg tcgccaaggt gaaggagatg cgcgacaacc
     1381 tcaacgacgc gacggtctcg ccctacaagc ccgtggcggt cgtgaacgac gtgagagagg
     1441 ccatcgtccg gcagatccgc gagaaccgag aggacttccc cggggtcgag gcggagaagc
     1501 tcccgatccg cacgtacccg aacgggaagc tcgccgccca cgtcctcggc tacgtcggcg
     1561 agatcaacgc cgacgagctc gaggcccccg agttccagga cgcgaagcct ccttacgagc
     1621 ccggcgacct catcgggaag tccggggtcg agcgcaccta cgaccgctgg ctgcgcggcc
     1681 gccccgagat caagcgcgtc gtcgtgaact cggccggcga ggtcgtggac caggcgatca
     1741 gacaagagga ggagccgggc cgcgacctca tcctgtcgat cgacgtcgag gcgcagaagc
     1801 tcgccgagag gtcactcgag agcgggatca tggcggcgcg cggcgccgct catccggccc
     1861 ccgacggtgc gatcgtcgtc atggacccga acaccggcgg catcgtcgcg atggcgagct
     1921 atccgacgta cgacccctcg atcctcgcgg acggcatcac cgagcgcgag tacgagcgga
     1981 tgagcgggag cccggacacg gccgaggacg acgtcctccc gaacagggcc acgcaggcgc
     2041 aggtgcctcc gggctcgacg ttcaaggtcg tgaccgccgg agcggccctc gcgaacgacg
     2101 tggtgaccgc gagcgactat ctgccgtgcc ccgggcaggt ctcctacggc gcgaccggga
     2161 tcaccaccgt gttcaacaac tggacgtcgg tcgacttcgg ctcgatcggg ttccccgagt
     2221 cgctcgagat ctcgtgcgac acgttctatt ccgagctcgg ctggcgcatg gaggagctgt
     2281 ggggggccgc gaacggcgac gagtccgagc gcttccagga ctacatgcgg cacgcggggt
     2341 tcggccggcc gaccggcatc gacctcccgc acgaggaaga gggacgcgtc cccgacgaga
     2401 agtggtgcga ggagttcgcg aagcagggtc tcggctgctt cgacgggtgg ctgcccggct
     2461 tcaccgtcaa catggcgatc gggcagggtg acctgacgac gaacccgctc cagatggcgg
     2521 tgacgtacgc cgcgctcgcc gccgactccg gcgaggtgtg ggagccgcac gtcgcccagc
     2581 agatcggccg tcccgacgcc gagacgggcg aggaggagat cctgcgtgag atcgagcccg
     2641 aggccttgcg gaggctcccg ctcgactcca ccgagatcgg cgtcatcagg caggggctcg
     2701 aggacgtggt gtccggcgcg aacgggaccg cggcgggcgc gttcgccggg ttcccgctcg
     2761 accagtaccc ggtcgccggc aagaccggta ccgcgcagat cggcgagtcc gaggccgacg
     2821 tcaactacgc gtggttcgtc tcgtacgccc ccgccgacga cccgcagtac gtcgtgtcgg
     2881 tctacctcgc caaggccggg cacggtggcg agaccgccgc acccgtggcg cgcgaggtct
     2941 tcgagggtct cttcggcatc gaccgggaga ccggcgtcac cctcggccag gacgcgtccg
     3001 gctgatggcg atggagggga tcttcggcga ggcggt
//
LOCUS       DASQDO010000428         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_534649, whole genome shotgun sequence.
ACCESSION   DASQDO010000428 DASQDO010000000
VERSION     DASQDO010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534649"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VEV43_11885"
     CDS             <1..387
                     /locus_tag="VEV43_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="daunorubicin ABC transporter ATP-binding
                     protein"
                     /protein_id="HYP24264.1"
                     /translation="YMEEADQLAGRIAIIDNGHIVADGTPSVMKAKVGDPTLRVAIGP
                     EEDRIAVEAALLECGEPAPGSADYVAIRLKGGASRIAAVARTLEDAGIAFTGLELLSP
                     SLDDVFQQATGRRLEGAAEPEAAPAR"
     gene            384..563
                     /locus_tag="VEV43_11890"
     CDS             384..563
                     /locus_tag="VEV43_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24265.1"
                     /translation="MSALDQYVALSRRAIVTTVRQPTAILPAMTFPLLFMALSSAAFE
                     RSTNLPGFPEVDSLL"
     gene            585..1160
                     /locus_tag="VEV43_11895"
     CDS             585..1160
                     /locus_tag="VEV43_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24266.1"
                     /translation="MQGTLFGSVAAGTAMATDIENGFFERLIAAPSSRTSILVGRVMG
                     AAVLAFVQAWVFMAVAMPFGLTVESGFVGMLSIALSAGVLGAGVGAIAMSFALRTGSA
                     EAVQGSFPLLFAGLFLSGAFFPRDLMDGWFKTVAGYNPFTYLIEGLRTQVIDGLDAGQ
                     LAVAIGVAAAIFVAGMLLSNAALRRRVAVAA"
     gene            1157..1921
                     /locus_tag="VEV43_11900"
     CDS             1157..1921
                     /locus_tag="VEV43_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24267.1"
                     /translation="MSGPRVAAAIAGRSLMLIPRVPSTFVPSLVMPLFLTVAMAGAFS
                     GLVLLPGFPADKSIDWFIPMTTLQGGAFAGITTGMGIARDLESGFYDRLLSSPATRSA
                     LLAGPLLASMLRAAIPLTILVVIALVSGANFHGGVATVGTLAIATLGMAFVGGAWSIG
                     LALKFKSMQVAPLMQTGIFLLVFLSTAQMPIHLLTGWLHTVARFNPMTNVLALGREGF
                     LGEVTWAGTWPGLVSLGGLALAATLFAASSMRKVTP"
     gene            complement(1935..2081)
                     /locus_tag="VEV43_11905"
     CDS             complement(1935..2081)
                     /locus_tag="VEV43_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24268.1"
                     /translation="MKTADEALKQIEETTRASLALTHPKTRVTAETRIRTSDETSSPV
                     IDMA"
     gene            complement(2156..2389)
                     /locus_tag="VEV43_11910"
     CDS             complement(2156..2389)
                     /locus_tag="VEV43_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYP24269.1"
                     /translation="MAVHAYVLVQTAVNAAHVAAEIRALDGVDQADDVSGPYDVIVRA
                     SASDMDTLGKLVVSRIQAVDGISRTLTCPIVQL"
     gene            2423..3154
                     /locus_tag="VEV43_11915"
     CDS             2423..3154
                     /locus_tag="VEV43_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYP24270.1"
                     /translation="MRPVAALLVVAAAALVLPGSGAEAQPVNPGVVSAGEKLYVESCA
                     SCHGVTGAGTESGPDLRDAGAAGADFYLRTGRMPLASSNDQAVRKEPAFSSEEIGQIV
                     AYVATLGDGPVIPYVSPEEPSLQDGAELYVANCAPCHGAAANGGAAGRNAIAPGLHRA
                     TPLEIAEAIVVGPGQMPVFGFSQEERDAVVAYVDHLDEADDPGGADIGGIGPVPEGFV
                     AWGVGMLALVVAALLIGSRRGARDS"
     gene            3151..>3929
                     /locus_tag="VEV43_11920"
     CDS             3151..>3929
                     /locus_tag="VEV43_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HYP24271.1"
                     /translation="MNPQRTASVAFAVSIAASLALVGVYWAGGDAQAEGVLLGLALGG
                     LGIGVVVWATRLMDSPVETEEREELASPPAVVDDPGDAGEEVARRSFLVRMLAAAGGT
                     LAAALAIPSLSLGPRPGQSLFRTAWAKGVRVVDSEGRALKPVDLVADSVTTVYPEGDV
                     GSEDAQTLVVRVEERLLELPGEGSEWAPEGCVGYSKICTHAGCPVGLYRAQAHELLCP
                     CHQSTFDVLRGAVPTFGPADRPLPQLPLGLDDEGYLIALCD"
BASE COUNT          480 a         1333 c         1471 g          645 t
ORIGIN      
        1 tacatggagg aggccgacca gctcgcgggc cgcatcgcga tcatcgacaa cggccacatc
       61 gtcgcggacg gcacgccttc ggtgatgaag gcgaaggtcg gcgacccgac gctgcgcgtc
      121 gcgatcgggc ccgaggagga ccggatcgcg gtcgaggccg cgctgctcga gtgcggcgag
      181 cccgctccgg gatccgccga ctacgtcgcg atcaggctga agggcggggc gagccgcatc
      241 gccgcggtgg ctcggacgct cgaagacgcg ggcatcgcgt tcaccggcct cgagctcctc
      301 tcgccgtcgc tcgacgacgt cttccaacag gccacggggc ggaggctcga gggggccgcg
      361 gagcccgagg ccgctccggc gcggtgagcg cgctcgacca gtacgtcgcg ctgagccggc
      421 gcgcgatcgt cacgaccgtc cggcagccca ccgcgatcct cccggcgatg acgttcccgc
      481 tgctgttcat ggcgctgtcg tcggcggcct tcgagaggtc gacgaacctg cccggctttc
      541 ccgaggtcga tagcttgctg tagttcctga tcgcgacgtc gatcgtgcag gggacgctgt
      601 tcgggtcggt cgccgcgggg accgcgatgg cgaccgacat cgagaacggc ttcttcgagc
      661 ggctgatcgc ggccccctcg tcgcgcacct cgatcctggt cggacgggtg atgggcgccg
      721 cagtcctcgc cttcgtccag gcgtgggttt tcatggcggt cgcgatgccc ttcgggctga
      781 ccgtcgagag cggcttcgtc gggatgctgt cgatcgcgct gtcggccggt gtgctcggcg
      841 cgggcgtcgg cgcgatcgcg atgtcgttcg cgctgcgcac ggggtcggcc gaggcggtgc
      901 aggggagctt cccgttgctg ttcgccgggc tgttcctgtc gggcgccttc ttcccgcgcg
      961 acctgatgga cgggtggttc aagacggtcg ccggctacaa ccccttcacc tacctgatcg
     1021 aaggactgcg gacgcaggtg atcgacgggc tcgacgccgg ccagctcgcg gtcgcgatcg
     1081 gcgtggccgc cgcgatattc gtggcgggga tgctgctgtc gaacgcggcg ttgcgccggc
     1141 gggtggcggt ggccgcgtga gcgggcctcg tgtcgcagcc gccatcgcgg gccgcagcct
     1201 catgctgatc ccgcgcgtgc cgtcgacgtt cgtcccgtct ctcgtcatgc cgttgttcct
     1261 gacggtggcg atggcgggag ccttctcggg gctcgtgctc ctgcccggct tccccgcgga
     1321 caagtccatc gactggttca tcccgatgac gacgctgcag ggcggtgcgt tcgcggggat
     1381 cacgaccggc atggggatcg cgcgcgacct cgagagcggc ttctacgacc ggctgctgtc
     1441 gagcccggcg acccgctcgg ctctgctcgc cggccccttg ctcgcgtcga tgctgcgtgc
     1501 cgctattccg ctgacgatcc tcgtcgtgat cgcgctcgtc tcgggggcga acttccacgg
     1561 tggcgtcgcg accgtcggga cccttgcgat cgcgacgctg gggatggcgt tcgtcggcgg
     1621 ggcgtggtcg atcgggctcg cgttgaagtt caagtcgatg caggtcgcgc cgttgatgca
     1681 gaccgggatc ttcctgctgg tgttcctgtc gaccgcgcag atgccgatcc acctgctgac
     1741 gggatggctc cacaccgtcg cccgcttcaa cccgatgacg aacgtgctcg cgctcgggcg
     1801 cgagggcttc ctcggcgagg tgacgtgggc cgggacctgg ccgggcctgg tctcgctcgg
     1861 gggcctggcg ctggcggcga cgctgttcgc cgcctcgtcg atgcggaagg tgacgccgta
     1921 acgggtcgat aaagtcacgc catgtcgatc acggggctgc tcgtctcgtc gctggtcctg
     1981 atcctcgtct ccgcggtcac cctggtcttc ggatgggtca acgccagcga ggccctcgtg
     2041 gtctcttcga tctgcttcag cgcctcgtcc gcggtcttca tggcgatggg tctctaccgg
     2101 tcgaggccgc ggcgagccgc gggacctgcg aggaagaagc cggagaagcg ggcggctaga
     2161 gctggacgat cgggcaggtg agcgtccggc tgatcccgtc gaccgcctgg atccgcgaga
     2221 ccaccagctt gccgagcgtg tccatgtcgc tcgcggacgc tcggacgatc acgtcgtagg
     2281 gcccggagac gtcgtcggcc tggtcgacgc cgtcgagggc gcggatctcg gccgcgacgt
     2341 gggccgcgtt caccgcggtc tggacgagga cgtacgcatg cactgccact gaccggattg
     2401 tagacgcggg tagcgtgggg cgatgcggcc ggtcgcagcg ctcctggtgg tagctgccgc
     2461 agccctcgtc ctaccgggga gcggggccga ggcgcagccc gtgaatcccg gcgtggtgag
     2521 cgccggagag aagctctacg tcgagtcgtg cgcctcgtgc cacggggtca cgggggccgg
     2581 gaccgagagc gggccggacc tgcgcgacgc gggggccgcg ggggccgact tctacctccg
     2641 gaccgggcgg atgccgctcg cgagctcgaa cgaccaggcc gtgcgcaagg agccggcgtt
     2701 ctcctccgag gagatcggtc agatcgtcgc ctacgtcgcg acgctcgggg acggcccggt
     2761 gatcccgtac gtctcgccgg aggagccgtc gctgcaggac ggggccgagc tctacgtcgc
     2821 gaactgcgcg ccgtgccacg gcgcggccgc gaacggcggt gccgccgggc gcaacgcgat
     2881 cgcgcccggg ctccaccgcg cgacgccgtt ggagatcgcg gaggcgatcg tcgtcgggcc
     2941 cgggcagatg ccggtgttcg ggttctcgca ggaggagcgc gacgcggtcg tcgcgtacgt
     3001 cgaccacctc gacgaagccg acgaccccgg cggcgcggac ataggcggga tcggtccggt
     3061 ccccgagggg ttcgtcgcgt ggggggtcgg gatgctagcg ctcgtcgtcg ccgcgctgct
     3121 gatcggcagc cggcgagggg cacgcgactc ttgaacccgc aaaggaccgc gtcggtcgct
     3181 ttcgccgtct cgatcgcggc ctcgctcgcg ctcgtcggcg tgtactgggc cggcggcgac
     3241 gcgcaggccg aaggcgtgct gctagggctc gctctcggcg ggctcggcat cggcgtcgtc
     3301 gtgtgggcga cgcgcctgat ggactcgccc gtcgagaccg aagaacgcga ggagctcgcg
     3361 tcgccaccgg cggtcgtcga cgatcccgga gacgcgggcg aggaggtagc gcggcgctcc
     3421 tttctcgtaa ggatgctcgc cgccgccggc gggaccctcg cggccgcgct cgctatcccg
     3481 tctctctccc tcggcccccg gccgggccag agcctctttc ggacggcgtg ggcgaagggc
     3541 gtgcgcgtcg tcgactcgga aggccgcgcg ctgaagcccg tcgacctcgt cgcggacagc
     3601 gtgaccacgg tctatcccga aggagacgtc gggtccgagg acgcgcagac gctcgtggtc
     3661 cgggtggagg agcgcctgct cgagctcccg ggggagggga gcgaatgggc ccccgaaggc
     3721 tgcgtcgggt actcgaagat ctgcacgcac gcgggttgtc cggtcggtct ctaccgggcc
     3781 caggcgcacg agctcctctg cccgtgccac cagtcgacgt tcgacgtgct gcgcggcgcg
     3841 gtcccgacgt tcggccccgc cgaccgtccg ttgccgcagc tcccgctggg gctcgacgac
     3901 gagggctacc tgatcgcgct ctgcgactt
//
LOCUS       DASQDO010000429         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_840895, whole genome shotgun sequence.
ACCESSION   DASQDO010000429 DASQDO010000000
VERSION     DASQDO010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840895"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VEV43_11925"
     CDS             complement(<1..225)
                     /locus_tag="VEV43_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HYP24272.1"
                     /translation="MVDSRLAEEGRVIRRRRECLGCTRRYTTFERAEEVPLLVVKRKG
                     DEEPFERSKVVVGIRRACKNRPVSGAAIEEL"
     gene            complement(262..987)
                     /locus_tag="VEV43_11930"
     CDS             complement(262..987)
                     /locus_tag="VEV43_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24273.1"
                     /translation="MKKLAAALSAALVVLSAPVSSGAGERPLSLHLKRHHRPGAPVIA
                     VVQNVSDETQRIEASWTITHAKTGAVVARNHWTKAERRLAPGETAVWEWDQREGSYSS
                     EPAPPGEAEERVRPGGYVLTAGEDDPRLTRRFQIGRFFTLGFEGRDDTFVVFVNERRP
                     IKAMKAQAAAEDKTLIVSGIVREGSRYNRDWSYKMGPGSIVLGEVFIEVCDAAPLYVE
                     RHKEEWLGERWCPWSSYVLKTGR"
     gene            complement(1002..1229)
                     /locus_tag="VEV43_11935"
     CDS             complement(1002..1229)
                     /locus_tag="VEV43_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24274.1"
                     /translation="MRVEGKGGLGRTSDQNCSECGAELKLRPASCPLCGRDTPFVPKE
                     RVAIDLDDYQNNVRLLRDELQRIRREDAEAV"
     gene            complement(1229..>2908)
                     /locus_tag="VEV43_11940"
     CDS             complement(1229..>2908)
                     /locus_tag="VEV43_11940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593173.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HYP24275.1"
                     /translation="NSSCNLASLNLMKFVDADDEFDVEGFKHAVEVVFLAQEISVGCA
                     SYPTKKIAENSFRFRQLGQGYANLGALLMSQGLAYDSDEGRAWAASITALMTGHCYAT
                     SARIAERVGPFAAFNENREPMLRVMDKHRAAAYEIADGLAPEPLLEAARASWDEVVAL
                     GADHGYRNAQATVLAPTGTIGLMMDCDTTGIEPDLALKKTKKLVGGGTMSIVNQTVPR
                     ALRKLGYDEAQVTEIVAYIDENSHVVGAPHLREEHMSVFDTAMGERSIHYMGHIRMMA
                     AAQPFLSGAISKTVNMPENVTIEDVEQAYVEGWRLGLKALAIYRDNCKTAQPLSITKK
                     EEKVDEKAKLATDLPEPVRRRLPRARRSMTFKFRVADTEGYLTAGEFPDGNPGEIFLR
                     VAKQGSTLAGIMDAFAVSISMGLQYGVPLSAYVKQFTNTRFEPSGMTDDPEFRIATSI
                     LDYVFRRLAVEYLSDDERHGLGIRTSGERQAEIEAKLEPMAEKANGNGNGHGNGNGHG
                     KKTEGDGVIVLGQVAGANVGSLPFCGTCGEEMIPAGSCFACRSCGSTSGCS"
BASE COUNT          427 a         1016 c          959 g          506 t
ORIGIN      
        1 gagctcttcg atcgcggccc cgctcaccgg gcggttcttg cacgctcgcc tgatgcccac
       61 gacgaccttc gagcgctcga acggctcctc gtcgcctttg cgcttgacga cgaggagcgg
      121 cacctcctcg gccctctcga acgtcgtgta cctgcgggtg cagccgaggc actcgcgccg
      181 gcgccggatc acccgtccct cctcggcgag acgggagtcg accaccttgt cctccccgac
      241 gccgcagtag ggacagcgca ttcagcgtcc ggtcttcaag acgtacgacg accagggaca
      301 ccagcgctcg ccgagccact cctccttgtg gcgttcgacg tagagcgggg ccgcgtcgca
      361 gacctcgatg aagacctctc cgaggacgat cgagcccggc cccatcttgt aggaccagtc
      421 gcggttgtaa cgcgacccct cccggacgat cccggagacg atcagggtct tgtcctcggc
      481 ggcggcctgg gccttcatgg ccttgatcgg ccggcgctcg ttgacgaaca cgacgaacgt
      541 gtcgtcgcgg ccctcgaagc cgagcgtgaa gaaacgaccg atctggaagc gacgtgtgag
      601 ccgggggtcg tcctcgccgg ccgtgagcac gtacccacca ggccggaccc tctcctccgc
      661 ctcgccgggc ggcgccggct cggagctgta ggagccctcg cgctggtccc actcccatac
      721 ggcggtctcg cccggagcga gcctgcgctc ggccttcgtc cagtggttcc gggcgacgac
      781 ggccccggtc ttcgcgtgcg tgatggtcca gctcgcctcg atgcgttggg tctcgtcgga
      841 gacgttctgc acgaccgcga tcaccggcgc tcccgggcga tggtggcgct tgaggtggag
      901 cgagagaggt ctctcacccg ccccagagga aaccggtgcg gacaggacga cgagggccgc
      961 ggacagcgcg gcggcaagct tcttcatgag gccagtatgc gtcagacggc ctcggcgtct
     1021 tccctgcgga tccgctgcaa ttcgtcccgg agaagcctga cgttgttctg gtagtcgtcg
     1081 aggtcgatgg cgacgcgctc cttcggcacg aagggcgtgt cgcggccgca cagtgggcag
     1141 gaggcgggac ggagcttgag ttccgccccg cactcgctgc aattctggtc cgaagtccgg
     1201 ccgaggccgc ccttcccctc gaccctcatc aggagcagcc gctcgtcgac ccgcaggagc
     1261 ggcaggcgaa gcacgacccc gccgggatca tctcctcgcc gcaggtcccg cagaacggca
     1321 gcgacccgac gttggcgccg gcgacctgac cgagcacgat cacgccgtca ccctccgtct
     1381 tcttgccgtg tccgttgccg ttgccatgac cgttcccgtt tccgttggcc ttctccgcca
     1441 tcggttcgag cttggcctcg atctcggctt ggcgttcgcc cgaagtacgt atgccgagcc
     1501 cgtgacgctc gtcgtccgag aggtactcga cggcgagccg ccggaagacg tagtcgagga
     1561 tcgacgtggc gatccggaac tcggggtcgt cggtcatgcc cgacggctcg aaccgggtgt
     1621 tcgtgaactg cttcacgtag gccgacagcg ggaccccgta ctggaggccc atcgagatcg
     1681 acaccgcgaa ggcgtccatg atcccggcga gcgtcgagcc ctgcttcgcg acccgcagga
     1741 agatctcgcc cgggttcccg tcggggaact cgccggcggt gaggtagccc tcggtgtcgg
     1801 cgacccggaa cttgaaggtc atcgatcggc gggcccgcgg gagcctccgg cggacgggct
     1861 ccggcagatc ggtcgcgagc ttcgccttct cgtcgacctt ctcttccttc ttcgtgatcg
     1921 acagcggctg cgcggtcttg cagttgtccc ggtagatcgc gagcgccttg aggccgagcc
     1981 gccacccttc gacgtacgcc tgctcgacgt cttcgatcgt cacgttctcg ggcatgttca
     2041 cggtcttcga gatcgcgccc gacaggaacg gctgcgccgc cgccatcatc cggatgtggc
     2101 ccatgtagtg gatcgagcgc tcgcccatgg cggtgtcgaa gacggacatg tgctcctcac
     2161 gcaggtgcgg cgcaccgacc acgtgggagt tctcgtcgat gtaggcgacg atctcggtca
     2221 cctgagcctc gtcgtagccg agcttgcgta gcgcccgcgg caccgtctgg ttgacgatcg
     2281 acatcgtccc gccgccgacg agcttcttgg tcttcttcag agcgaggtcc ggctcgatcc
     2341 ccgtcgtgtc gcagtccatc atcagcccga tggtcccggt cggtgcgagg acggtcgcct
     2401 gcgcgttgcg gtagccgtgg tccgccccga gagcgacgac ctcgtcccac gacgcccggg
     2461 cagcctcgag cagcggctcg ggcgcaagcc cgtccgcgat ctcgtaagcc gcggcccggt
     2521 gcttgtccat cacgcgcagc atcggctcgc ggttctcgtt gaaagcagcg aacggcccca
     2581 cacgctcggc gatccgggcc gaggtcgcgt agcagtggcc cgtcatcagc gcggtgatcg
     2641 acgcggccca cgcccggccc tcgtcggagt cgtaagcgag gccctggctc atcagcagcg
     2701 cgccgaggtt cgcgtagccc tggccgagct ggcggaagcg gaaggagttc tcggcgatct
     2761 tcttcgtcgg gtacgaggcg cacccgaccg agatctcctg tgcgaggaac acgacctcga
     2821 ccgcgtgctt gaagccctcg acgtcgaact cgtcgtcggc gtcgacgaac ttcatgaggt
     2881 tcagcgaggc caggttgcac gacgagtt
//
LOCUS       DASQDO010000430         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_902477, whole genome shotgun sequence.
ACCESSION   DASQDO010000430 DASQDO010000000
VERSION     DASQDO010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902477"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2606)
                     /locus_tag="VEV43_11945"
     CDS             complement(<1..>2606)
                     /locus_tag="VEV43_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP24276.1"
                     /translation="DAADLSLTKTHTGAFVRGGTGTYRLTVSNAGPATSGAVTITDTL
                     PAGLTYRSFSGTGWSCSGCSSASQTVTLTRAGIANGASSFVDLTVDVAAGAAASLTNN
                     ASVSQATSATLNDPAAGNNTASDATTTANPGEADLVVTKSRDEVMTPGTEETYTIRVR
                     NDGPGTVVGPVTMTDVLPQGLTFVSATGDGWSCSATGQTVTCTRPGNLAAAEAAPPIT
                     LVVSVAPDAPARSTNRTSATGSVDNDPNDPNNVDVLDVVRVGSADLWIEKSHTGSFSR
                     GGAGKYTILVGNRGPNAAETPRVVDTLPAGLTYTSATGTGWTCSNSGQTVACDAASDL
                     AAEATAPALTINVAVAAGAASTVMNRATVCSVQDPGSVTGCPDQERGTSEVAPSDNSA
                     EDLTATTQPADLALSKVASVTSVAAGTPFDYTLTVTNNGPNPATNVTVVDTLPVYVDA
                     SSITTNPGPSNTSTTPYCDVTNREVTCALGNLAATAGSNTATATITARALPTAAGRTI
                     VNSATVFSDLGDPTSGNDGASAPVTVTGTLVNSAPVAAAKTLTVSHRSVTGGDVVLSA
                     TDSDGDPLTYALVGADGGAAHGTVTVSGNVATYVPDGDFTGTDTFEYRAHDGAESSAP
                     ATVTVTVTNGAPSAADRAATVPHRSTGAAIVLDGEDPDGDALTYTLEGAHGGASHGTV
                     TISGATATYVPAGDFTGTDTFRYRSGDGAATSAPATVTITLTNSAPALGSAALSPRTV
                     GTGGTLTATAVSPSDADGDDLTYTYVWTRTRAGDTQTLDTETTSSTTDSLALTGSAVG
                     DEIEVTITANDGHTDSPARTDAVVVGNTAPTAAAASASTDEDTTKTLTLSGSDADGDD
                     LTFEVTSLPDHG"
BASE COUNT          297 a          868 c         1024 g          417 t
ORIGIN      
        1 gcccgtggtc ggggagcgac gtgacctcga acgtcaggtc gtcgccgtcg gcgtccgagc
       61 cggagagcgt gagcgtcttc gtcgtgtcct cgtccgtgga cgcgctcgcg gccgcggccg
      121 tgggtgcggt gttgccgacg acgacggcgt ccgtccgcgc cggcgagtcc gtgtggccgt
      181 cgttcgcggt gatcgtcacc tcgatctcgt cgccgaccgc agagccggtc agggcgagcg
      241 agtcggtcgt cgagctcgtg gtctcggtgt cgagggtctg cgtgtcgccg gccctcgtcc
      301 tcgtccagac ataggtgtag gtgaggtcgt cgccgtcggc gtccgaaggt gacaccgcgg
      361 tcgcggtgag cgtcccgccg gtgccgaccg tccgcggcga cagcgcagcg gagccgagcg
      421 ccggggcgga gttggtgagc gtgatcgtga cggtcgcggg ggccgaggtg gccgcgccgt
      481 cgccggaccg gtaccggaac gtgtcggtgc cggtgaagtc acccgccggc acgtaggtcg
      541 cggtggctcc ggagatcgtg accgttccgt gggacgcacc tccgtgcgcg ccctcgagcg
      601 tgtacgtgag cgcgtcgccg tcggggtcct cgccgtcgag gacgatcgcg gcccccgtgc
      661 tgcggtgcgg gacggtcgcg gcgcggtcgg cggcggacgg agccccgttc gtgacggtca
      721 cggtcacggt cgcgggggcc gagctctcgg cgccgtcgtg cgcgcggtac tcgaacgtgt
      781 cggtgccggt gaagtcgccg tcgggcacgt aggtagcgac gttgccggag accgtgacgg
      841 tcccgtgagc cgcccccccg tccgcaccca cgagcgcgta ggtcaagggg tctccgtcgg
      901 agtccgtcgc gctcaggacg acgtcaccgc cggtgacgct gcggtgcgag accgtcagcg
      961 tcttcgccgc tgcgacgggg gccgagttca cgagcgtccc cgtcacggtg accggcgcgc
     1021 tcgcgccgtc gttgccggag gtcgggtcgc cgaggtcgct gaacaccgtc gccgagttga
     1081 cgatcgtgcg tcccgccgcg gtcggcagag cccgcgcggt gatcgtcgcc gtcgcggtgt
     1141 tcgaccctgc ggtggccgcc aagttcccca gcgcgcacgt gacctcgcgg ttcgtgacgt
     1201 cgcagtacgg ggtcgtcgag gtgttgctcg ggccggggtt cgtcgtgatc gacgaggcgt
     1261 cgacgtacac cggaagcgtg tcgacgaccg tgacgttcgt cgcggggttc gggccgttgt
     1321 tggtgaccgt cagcgtgtag tcgaacgggg tccctgccgc gaccgacgtg accgaagcga
     1381 ccttggacaa ggccaggtcg gccggctgcg tagtcgccgt caggtcctcg gccgagttgt
     1441 cggacggggc gacctcggac gtgccgcgct cctggtcggg gcagccggtg accgagccgg
     1501 ggtcctggac cgagcagacc gtcgcgcggt tcatgacggt ggaggccgcg cccgcggcga
     1561 ccgcgacgtt gatcgtcaga gcaggcgccg tcgcctcggc ggcgaggtcc gacgctgcgt
     1621 cgcacgccac ggtctggcca ctgttcgagc acgtccatcc ggtgccggtg gccgacgtgt
     1681 aggtgaggcc ggcgggaagg gtgtcgacga cgcgcggcgt ctccgcggca ttcggccccc
     1741 tgttcccgac gaggatcgtg tacttgcccg caccgccgcg cgagaagctg ccggtgtgcg
     1801 acttctcgat ccacaggtcg gcggacccga cgcgcacgac gtcgagcacg tcgacgttgt
     1861 tcgggtcgtt cgggtcgttg tcgacggagc ccgtggcgga cgtcctgttc gtgctccgcg
     1921 ccggcgcgtc cggagcgacc gagaccacga gcgtgatcgg gggagcggcc tcggcggccg
     1981 cgaggttgcc ggggcgcgtg cacgtcacgg tctgcccggt agcgctgcac gaccagccgt
     2041 cgccggtagc ggagacgaac gtgaggccct gcggcagcac gtcggtcatc gtgacggggc
     2101 ccacgaccgt gccgggcccg tcgttgcgga cgcggatcgt gtaggtctcc tcggtccccg
     2161 gcgtcatgac ctcgtcgcgc gacttcgtga cgacgaggtc ggcctcgccg gggttcgccg
     2221 tcgtggtcgc gtccgaggcg gtgttgttgc ccgccgcggg gtcgttgagc gtcgcgctcg
     2281 tcgcctgcga gaccgatgcg ttgttcgtga gggaagcagc cgcgcccgcg gcgacgtcga
     2341 cggtgaggtc gacgaacgag gacgcgccgt tggcgatccc ggcccgcgtc agcgtgacgg
     2401 tctgcgaggc cgagctgcag cccgagcagc tccagccggt cccgctgaac gagcggtacg
     2461 tcagacccgc cgggagcgtg tccgtgatcg tgacggcgcc ggacgtcgcc ggccccgcgt
     2521 tgctcaccgt gaggcggtat gtgccggtcc cgccgcgcac gaacgcgccg gtgtgggtct
     2581 tggtgagcga gaggtcggcc gcgtcg
//
LOCUS       DASQDO010000431         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_596573, whole genome shotgun sequence.
ACCESSION   DASQDO010000431 DASQDO010000000
VERSION     DASQDO010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596573"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VEV43_11950"
     CDS             complement(<1..220)
                     /locus_tag="VEV43_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24277.1"
                     /translation="MTSTGSGVPPPPAAPPSFLESRRFSILCEIQPPRRPDIEIVRDQ
                     IHVLKPVVDAFLIPDNHLGRATVSSVSVA"
     gene            complement(254..1342)
                     /locus_tag="VEV43_11955"
     CDS             complement(254..1342)
                     /locus_tag="VEV43_11955"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYP24278.1"
                     /translation="MAREGTTPSFASDRELVQAFQRGNPGAYDEIYRLYAPRIRRVCA
                     RMLKNPQDVDEATQETFLKAFQALGRFNGQYQLGAWLSRIASNVCLDHIRSRARTAHQ
                     VALSDEHEVAGTERGPEEIVVGDNPRIQEAIAEIQPLHAQALKLRALGGLSHREMAGE
                     LHMTPAQVKALLHRARSSFRRAWDRAEGWALAPLAGIRGFFSDKSGTSQASSNLIGFT
                     PSLSPLLAERVAASAVIVAVALSGAPTAAPEAPAEEQRRRPVAVAPIVDDLAKPPARE
                     RATSPAPRSHVAAAPAPVRSEPAADHDPVTKVLEAVGEQSTKLPKDKGGDDGGEDGGD
                     DGGLGTPGPASAKPVVRKVKKVVQDLTH"
     gene            complement(1425..1715)
                     /locus_tag="VEV43_11960"
     CDS             complement(1425..1715)
                     /locus_tag="VEV43_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24279.1"
                     /translation="MNPMLKKIVAAVAVKEAIEKVQEMRKPKPSLWSRISPLVFVAAI
                     GGALFYLGKSGKLGGMKDKSIGSGGSSNGSTSWDGGSNSAAPAAPSPSTSGV"
     gene            1846..2289
                     /locus_tag="VEV43_11965"
     CDS             1846..2289
                     /locus_tag="VEV43_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYP24280.1"
                     /translation="MIVFEEFEAKTGGRLDTLDITDEVHDTVRASGVRRGSVLVFSPH
                     TTCCVIISTPGARMYEVLQQTMDAIAPDGDYYAHDDLDIRTENLVEDEPANAPAHIVH
                     AFMGKTSECIPIIDGGAVLGDGQRVLFVELDSSRERRYCIQVLGE"
     gene            2286..>2841
                     /locus_tag="VEV43_11970"
     CDS             2286..>2841
                     /locus_tag="VEV43_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYP24281.1"
                     /translation="MSAPARPQWLADDMARIEDLLFEAAGASQLALVAEASTHLIKAG
                     GKRLRPALVMISSRAGEAGRRATDLAATAIELVHLATLYHDDVIDETETRRGVPTAHA
                     KWGTDVAVLAGDYLFASGSALGADAGGEVPGILARAIAEVCEGQIAETEAAGDPLRPV
                     GDFLDTIGKKTGALFRASCDLGTST"
BASE COUNT          408 a          991 c          992 g          450 t
ORIGIN      
        1 gcgcgaccga gacgctcgag accgtcgcgc ggcccaggtg gttgtccggg atcaggaacg
       61 cgtcgacgac gggcttcagc acgtggatct ggtcgcggac gatctcgatg tcgggccgcc
      121 gcgggggctg gatctcgcag aggatgctga agcgccggga ttcgaggaac gaaggcggcg
      181 ccgctggagg aggcggaacc ccggaccccg tgcttgtcat ggcctcggat ataccaggcc
      241 gcgggttacc cggtcagtgc gtgaggtcct ggacgacctt cttgaccttg cggacgaccg
      301 gcttggcgct cgcggggccc ggcgtgccga ggccgccgtc gtcgccaccg tcctcgccgc
      361 cgtcgtcacc gcccttgtcc ttcgggagct tcgtgctctg ctcgccgacg gcctcgagga
      421 ccttcgtcac ggggtcgtga tcggcggcgg gctcggacct aaccggggcg ggagcggccg
      481 cgacgtggct ccgcggggcc gggctcgtcg ccctttcgcg cgccggtggc ttcgccaggt
      541 cgtcgacgat cggggctacg gcgacgggtc gcctgcgctg ctcctccgca ggagcctccg
      601 gcgccgccgt gggcgccccg gacagcgcga ccgccacgat cacggccgat gccgcgacgc
      661 gctcggccag cagcgggctc agcgacggcg tgaacccgat gaggttcgac gacgcctggg
      721 acgtccccga cttgtcggag aagaaccccc ggatcccggc cagcggggcg agcgcccagc
      781 cctcggccct gtcccacgcc ctgcggaacg agctgcgcgc ccggtggagc agcgccttca
      841 cctgtgcggg cgtcatgtgg agctcgcccg ccatctcgcg gtgggacagc ccaccgagag
      901 cgcggagctt gagggcctgc gcgtggagcg gctggatctc ggcgatcgcc tcttggatgc
      961 gcgggttgtc gccgaccacg atctcctccg gcccgcgctc ggtccccgcg acctcgtgct
     1021 cgtcggacag ggccacctgg tgcgcggtcc gggcccggga acggatgtgg tcgaggcaga
     1081 cgttggaggc gatccgcgac agccacgccc cgagctggta ctggccgttg aaccggccca
     1141 gtgcctggaa ggccttgagg aacgtctcct gggtggcctc gtcgacgtcc tgggggttct
     1201 tcagcatgcg ggcacagacg cgccggatcc tcggcgcgta gagccggtag atctcgtcgt
     1261 acgcgccggg attgcctcgt tggaaggcct gaaccagctc gcggtcggac gcgaacgaag
     1321 gtgtagttcc ctcgcgggcc atattcagat ggttcgccct gttttccaca tttcctacaa
     1381 cgacgcagac ggccccgtgg tggttccgga cacggggatt ttgtctagac gccgctcgtc
     1441 gagggcgagg gggccgcggg ggccgcggag ttgctgccgc cgtcccacga ggtcgacccg
     1501 ttggagctcc cgccggagcc gatggacttg tccttcatcc cgccgagctt gccgctcttg
     1561 cccaggtaga agagcgcgcc gccgatcgca gccacgaaca ccaggggcga gatcctcgac
     1621 cacagcgacg gcttcggctt ccgcatctcc tgtaccttct cgatcgcttc cttgaccgcg
     1681 accgcggcga cgatcttctt cagcatcggg ttcattggtc ttcctccgtc ttaataggtg
     1741 ccgtaccgag tagtcctacc ccgcgccggt tcttttgaac gcacgttcag aggcccctaa
     1801 cgcccgtcca atgccgtgcg taccggtatg cgagaaaatg accgcatgat cgtcttcgaa
     1861 gagttcgagg caaagaccgg cgggcggctc gacacgctcg acatcaccga cgaggtgcac
     1921 gacaccgtac gtgcgtcggg tgtccgccgg ggaagcgttc tcgtgttctc cccccacacc
     1981 acgtgctgcg tgatcatctc gacgccgggc gcccgcatgt acgaggtgct ccagcagacg
     2041 atggacgcga tcgcgccgga cggcgactac tacgcgcacg acgacctcga catccgcacc
     2101 gagaacctcg tagaggacga gccggcgaac gcgccggcac acatcgtcca cgccttcatg
     2161 ggcaagacgt cggagtgcat cccgatcatc gacgggggcg cggtcctcgg cgacggccag
     2221 cgcgtgctgt tcgtcgagct cgactcgtcg cgggagcggc ggtactgcat ccaggtcctg
     2281 ggcgagtgag cgcacccgcc cggccgcagt ggttggcgga cgacatggcg cggatcgaag
     2341 acctgttgtt cgaggccgcg ggcgcgtccc agctcgcgct cgtcgcagag gcgtcgaccc
     2401 acttgatcaa ggccggcggc aaacggctgc gtccggcgct cgtgatgatc tcttcccgcg
     2461 cgggcgaggc cggacgccgc gccaccgacc tggcggccac cgcgatcgag ctggtccacc
     2521 tcgcgacgct gtaccacgac gacgtcatcg acgagaccga gacccgccgc ggcgtcccga
     2581 ccgcgcacgc taagtggggc acggacgtcg ccgtcctggc gggcgactac ctcttcgcga
     2641 gcgggagcgc gctcggcgcc gacgccggcg gtgaggtccc ggggatcctc gcgcgtgcga
     2701 tcgccgaggt ctgcgagggc cagatcgccg agaccgaggc cgccggcgac ccgctgcggc
     2761 ccgtcggcga cttcctcgac acgatcggca agaagaccgg cgcgctgttc cgtgcgtctt
     2821 gcgacctcgg cacgtcgacg t
//
LOCUS       DASQDO010000432         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_45765, whole genome shotgun sequence.
ACCESSION   DASQDO010000432 DASQDO010000000
VERSION     DASQDO010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45765"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..482
                     /locus_tag="VEV43_11975"
     CDS             159..482
                     /locus_tag="VEV43_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24282.1"
                     /translation="MPYTSLWSVASEGSILSIASRGSILSIGSAGSILSIGSAGSIAS
                     CLSVGSAGSFASALSWRSSGSLLDAEGRGSVMGQPLPRRSALFVAAALVAGAAAVVAA
                     SRSAE"
     gene            486..>2164
                     /locus_tag="VEV43_11980"
     CDS             486..>2164
                     /locus_tag="VEV43_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP24283.1"
                     /translation="MEDHPDLAAEQRYVDDSYACLERMRAYAAELLERKKVTDTVDDE
                     ILRWHLQERVASLADSAAPLVFGRIDQEHEDPLYVGRRHVRDPDGEVKVVDWRARAAA
                     PFYRATIRDPMDVHRRRRFAMEGRELLDLFDEFLDDPDSVVETSHGGVPDPLLAEIGR
                     ARTGQMRDIVATIQAEQDEIIRSPIEELVLVQGGPGTGKTAVGLHRTAFLLYEHRATL
                     ERERVLIVGPNRLFLAYISNVLPSLGETAVYQTTVLGLAAGMQKVRRDDHPAVTRVKG
                     DLRMAEVLRRAVWSQVQAAESPLRALVRGVSIDLPPDDVNAALLEAMRARSYREGRRL
                     FHELVGRRILAEREVDVYALGLEAPEFLDVALRRSPLERQLRGLWPNAGHESLVRRLL
                     TNKAALAAAAEGLLSDDEQTLLRAHSRRELKELGWSTADVPLVDECDALVNGPPARYG
                     HAVVDEAQDLSAMELRMIARRTRAASMTVLGDLAQATAAGSQSSWDDVLEAFGRPPNA
                     TLRELTMGYRVPSEVMDFANRLLPLAAPGTRASTSVRRSQEPPRLVHTTDLL"
BASE COUNT          288 a          812 c          740 g          324 t
ORIGIN      
        1 cgtctatgca cgacgcgtcc tgcgcccgtg ccgtcgccac cgggaacacc gcaagcccta
       61 ccgaagctgc cgccaacccc accagtcgct tcctcatcgc cgccaccttc cgcctggcct
      121 tcgggacccc cggagtatcg cgcggctagg ctttccgcat gccctacacc tcgctgtggt
      181 ccgtcgcgtc cgaggggtcg atcctctcga ttgcgtcgcg cggctccatc ttgtcgatcg
      241 ggtcggccgg gtcgatcctc tcgatcgggt ccgccggatc gatagcgtcg tgcctgtccg
      301 tcggctcggc cggctcgttc gcgtcggctc tgtcgtggcg ctcgtcgggg tcgttgctgg
      361 atgccgaggg ccggggatcg gtgatggggc aacccctgcc gagaagatcc gcgctgttcg
      421 tcgccgcagc tctcgtcgcc ggcgccgcgg ccgtcgtcgc agcgtcccga agcgccgagt
      481 aacccgtgga ggaccatccc gacctcgctg cggagcagcg ctacgtcgac gactcctacg
      541 cctgcctcga gcggatgcgc gcctacgcgg ccgagctcct cgagcgcaag aaggtcacag
      601 acaccgtcga cgacgagatc ctccggtggc acctgcagga gcgcgtcgcg tcgctcgccg
      661 actccgccgc acctctggtc ttcggacgca tcgatcaaga acacgaggat ccgctctacg
      721 tcggccggcg tcacgtccgc gacccggacg gcgaggtcaa ggtcgtcgac tggcgggcgc
      781 gggccgcggc ccccttctat cgcgccacga tccgcgaccc gatggacgtg caccggcggc
      841 gccgcttcgc gatggagggc cgcgagctcc tcgacctctt cgacgagttc ctcgacgacc
      901 cggactcggt cgtcgagact tcgcacggcg gcgttcccga cccgctgctc gcggagatcg
      961 ggcgcgcccg caccggacag atgcgcgaca tcgtcgcgac gatccaggcc gagcaggacg
     1021 agatcatccg cagccctatc gaggagctcg tgctcgtcca gggggggccg ggcaccggca
     1081 agacggcggt cggcctccac cgcaccgcgt tcctgctgta cgagcaccgg gcgacgctcg
     1141 aacgagagcg cgtgctgatc gtggggccga accggctctt cctcgcgtac atctcgaacg
     1201 tcctgccgtc cctcggggag accgccgtgt accagacgac cgtcctcggg ctcgcggccg
     1261 gcatgcagaa ggtccggcgc gacgaccatc ccgcggtgac gcgcgtgaag ggcgacctgc
     1321 gcatggcgga ggtgctccgg cgcgcggtct ggtcccaggt acaggccgcg gagtcaccgt
     1381 tgcgcgcgct cgtacgcggc gtatcgatcg acctcccccc ggacgacgtg aacgcggcgc
     1441 tgctcgaggc gatgcgggcg cgctcctacc gcgagggtcg ccggctgttc cacgagctcg
     1501 tcggccggcg catcctcgcc gagcgcgagg tcgacgtcta cgcgctcggt ctcgaagcgc
     1561 cggagttcct cgacgtagct ctgcggaggt cgccgctcga gcgtcagctg cgcgggctgt
     1621 ggccgaacgc cggccacgag tcgctggtcc ggcgtctgct gacgaacaag gccgccctcg
     1681 ccgccgcggc cgagggcctc ctgtccgacg acgagcagac gctgctgcgg gcgcactctc
     1741 ggcgcgagct caaggagctc ggctggtcca ccgccgacgt cccgctcgtc gacgagtgcg
     1801 acgcgctcgt gaacggcccg cccgcgcgct acggccacgc cgtcgtcgac gaggcccagg
     1861 acctctcggc gatggagctg cggatgatcg cgcggcgcac gagggccgcg tcgatgacgg
     1921 tcctcggcga cctcgcgcag gccacggccg ccggctcgca gagcagctgg gacgacgtcc
     1981 tcgaagcgtt cgggcgcccg ccgaatgcga cgctgcgcga gctgacgatg ggataccgcg
     2041 tcccctcgga ggtcatggac ttcgccaacc ggctgctgcc cctggcggct cccgggacgc
     2101 gcgcttcgac gtcggtccgc cgctcgcagg agccgccgcg tctcgtccac acgaccgacc
     2161 tcct
//
LOCUS       DASQDO010000433         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_658624, whole genome shotgun sequence.
ACCESSION   DASQDO010000433 DASQDO010000000
VERSION     DASQDO010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658624"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VEV43_11985"
     CDS             complement(<1..431)
                     /locus_tag="VEV43_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYP24284.1"
                     /translation="MRTVFFGTPAWAVPSLRALVESPVEVAAVVTNPDKPAGRGMELR
                     PSPVKEEALRHGLEVLQPLRAREPELHERLAAVAPDVAVVVAYGSILPGPLLEVPRLG
                     FVNLHFSLLPAYRGAAPVPRAVMDGVDVSGVSIMVLTEGMD"
     gene            complement(428..970)
                     /gene="def"
                     /locus_tag="VEV43_11990"
     CDS             complement(428..970)
                     /gene="def"
                     /locus_tag="VEV43_11990"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYP24285.1"
                     /translation="MAILPIRTFGDPVLRQRAREVERVTEVHRRLVADMLETMREAPG
                     VGLAGPQVGVLERVFVYEVEDRHGAVFNPVIVRRSAEMETGEEGCLSIPGLTYDVDRH
                     SSVAIEGLDADGNPVSIDAGDDDFLARVFQHEIDHLDGVLFLDRLPDDTRKKAMRSLR
                     DKLLGLPTPQPQPAPREEAL"
     gene            complement(987..>1649)
                     /locus_tag="VEV43_11995"
     CDS             complement(987..>1649)
                     /locus_tag="VEV43_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24286.1"
                     /translation="AGTERLAEQLAKSFPRATVGRVDADVLAATDERGPSPDVYVTTW
                     IGTKPALRPDVDLVGVLDADALLRRPDWRAAEDGYQALAAMAEWAGPARDGGRLVLQT
                     DEPAHHAIQAVVRGDYDFFLERELEARRELSYPPFSELVKLTGSGPGAADLMQQAVDV
                     CREAGAKVLGPISVRPPGASEDELQTLVKCRDALAVTPGRRGILARAPAGSRLRVDVD
                     PR"
BASE COUNT          227 a          570 c          627 g          225 t
ORIGIN      
        1 tcgtccatgc cttcggtcag caccatgatc gacacgccgc tcacgtcgac gccgtccatc
       61 accgcgcgcg gcacgggggc cgcgccccgg taagcgggca gcaaagagaa gtggaggttg
      121 acgaacccga gccgcgggac ctcgagaagg gggccgggca ggatgctccc gtaggcgact
      181 acgacggcga cgtcgggggc gacggcggcg aggcgttcgt gcagctccgg ctcgcgtgcg
      241 cgcaggggct ggagcacctc gaggccgtga cgaagcgcct cctccttgac cggcgacggg
      301 cgcagctcca tcccgcggcc cgcgggcttg tcggggttcg tcacgaccgc ggcgacctcg
      361 accggggact cgacgagtgc ccggaggctc ggcaccgccc aggccggcgt accgaagaag
      421 acggttctca gagagcttcc tcgcgcgggg ccggctgggg ctgcggggtc gggaggccga
      481 ggagcttgtc gcgcaggctc ctcatcgcct tcttgcgggt gtcgtcgggc agcctgtcca
      541 gaaagaggac gccgtcgagg tggtcgatct cgtgctggaa gacgcgggcg aggaaatcgt
      601 cgtcgccggc gtcgatcgag accgggttgc cgtccgcgtc gaggccctcg atggcgacgg
      661 acgaatggcg gtcgacgtcg taggtgaggc ccgggatcga caggcacccc tcctcgccgg
      721 tctccatctc tgcggagcgg cggacgatca cggggttgaa gacggcgccg tggcggtcct
      781 cgacctcgta gacgaagacc cgttcgagga cgcccacctg ggggccggcg aggccgacgc
      841 cgggggcctc gcgcatggtc tcgagcatgt cggcgacgag ccggcggtgg acctcggtta
      901 cccgttcgac ctcgcgcgca cgctggcgga ggacggggtc tccgaacgtg cggattggaa
      961 ggatggccaa gctcaggtct ctccgtctaa cgagggtcta cgtcgacgcg cagccggctc
     1021 cccgccggcg cccgggcgag gataccccgc cgccccggcg tgacggcgag ggcatcgcgg
     1081 cacttcacga gggtctggag ctcgtcctcg gacgctcccg gcgggcgcac ggagatgggg
     1141 ccgaggacct tcgcgccagc ctcccggcag acgtcgacgg cctgctgcat gaggtccgcc
     1201 gctcccgggc cgctgccggt gagcttcacg agctcggaga acggcgggta gctcagctcg
     1261 cgccgcgcct cgagctcgcg ttccaggaag aagtcgtagt cgccgcgcac gacggcctgg
     1321 atcgcgtggt gcgcgggctc gtcggtctgg aggacgagac ggcctccgtc gcgcgccggc
     1381 ccggcccact cggccatcgc cgcgagggcc tggtagccgt cctcggcggc gcgccagtcc
     1441 gggcggcgca ggagcgcgtc ggcgtcgaga accccgacca ggtcgacgtc gggacggagc
     1501 gcgggcttcg tacctatcca cgtcgtgacg tagacgtccg ggctcggacc ccgctcgtcc
     1561 gtcgccgcga ggacgtccgc gtcgacccgg ccgaccgtgg cccgggggaa cgacttcgcc
     1621 agctgctcgg ccaggcgttc ggttcccgc
//
LOCUS       DASQDO010000434         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1147617, whole genome shotgun sequence.
ACCESSION   DASQDO010000434 DASQDO010000000
VERSION     DASQDO010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147617"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VEV43_12000"
     CDS             <1..1514
                     /locus_tag="VEV43_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYP24287.1"
                     /translation="PKKDKYAKLNLAILAEPDLGRVKVAGTKRHLQQAPGTGRQAGSA
                     FKPFALAAAIEDGVPLSKRYDGSGPFVVPGADNGGDYTVQNYEGGQFGEITLLEATVN
                     SVNVVYAQLVDEIGADPVVDIAQRMGIRTPLMPYFSAVLGTNEVNPLGMAASYGTLAN
                     NGDHHPPVAVTKIVDVSNEEKKKVIYRDETEIVDGVLDPGAAYLTTSALEEVVKRGTG
                     THALAIGRPAAGKTGTAQEYRDAWFVGYTPNLVAAVWVGYPQGQIEMKTSCLGSTAGI
                     GGIPECLPTRITVTGGSWPTDIWTSFMLRALSGYPVEAFDVPAAGIVRVTIDTRNGCL
                     ADKFTPAEYVGTGVFASGTEPEESCREPGDRKLVENLIGFPRGEAIRILEGEGFVVAT
                     EAVPLGSYPPGTVVDQSPSGGSRVPAGSTVTLYIASEDVEDAATVPGVLGMTRGEAES
                     RIRSAGFDVNVVVESESSRGQAKKNAGRVWKQAPGGGAELQEGDTVTIWVNPG"
     gene            complement(1999..>2426)
                     /locus_tag="VEV43_12005"
     CDS             complement(1999..>2426)
                     /locus_tag="VEV43_12005"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HYP24288.1"
                     /translation="LPWGLWPLALAYVAVPLATGTLIGRGTLEGQAWAAAFTGPNPAP
                     RAWDHFFSSRPDGWVRLRLKRGVWIGGIYGPPDVGLPSYASGYPEAQDLFLAETVEVD
                     PETGAFILAAGEPILRGVSLLIRWDEVEMLEFGDSGSAA"
BASE COUNT          412 a          801 c          865 g          348 t
ORIGIN      
        1 cgccgaagaa ggacaagtac gcaaagctca acctcgcgat cctggccgag ccggacctcg
       61 gccgcgtcaa ggtcgccggc accaagaggc atctgcagca ggcgccgggg acggggcggc
      121 aggcgggctc ggcgttcaag ccgttcgcgc tcgcagcggc gatcgaagac ggcgtcccgc
      181 tctcgaagcg ctacgacggc agcggcccgt tcgtcgtccc cggcgccgac aacggcgggg
      241 actacacggt gcagaactac gaaggcggcc agttcggcga gatcacgctg ctcgaggcta
      301 cggtcaactc cgtcaacgtc gtgtacgcgc agctcgtcga cgagatcggg gcggacccgg
      361 tcgtcgacat cgcgcagcgg atgggcatcc ggaccccgct gatgccttac ttctcggcgg
      421 tgctcggcac gaacgaggtc aacccgctgg ggatggcggc gtcttacggg acgctggcga
      481 acaacggcga ccaccacccg ccggtcgcgg tgacgaagat cgtcgacgtg agcaacgagg
      541 agaagaagaa ggtcatctac cgcgacgaaa ccgagatcgt cgacggcgtg ctcgacccgg
      601 gggccgccta cctgacgacg tcggcgctcg aggaggtcgt gaagcgcggg acgggcacgc
      661 acgcgttggc gatcggccgg cctgcggcgg gcaagaccgg cacggctcag gaataccgcg
      721 acgcgtggtt cgtcggatac acgccgaacc tcgtcgccgc cgtctgggtc gggtacccgc
      781 agggacagat cgagatgaag acgtcctgtc tcgggagcac ggccggcatc ggaggcatcc
      841 ccgagtgtct gccgactcgc atcacggtta cgggcgggtc gtggccgacg gacatctgga
      901 cctcgttcat gctgcgcgcg ctctccgggt atccggtcga ggccttcgac gtgccggccg
      961 cgggcatcgt gcgtgtgacg atcgacacgc gcaacggctg cctcgccgac aagttcacgc
     1021 ccgccgagta cgtcgggacc ggcgtgttcg cgagcgggac cgagcccgag gagtcgtgcc
     1081 gcgagccggg cgaccgcaag ctcgtcgaga acctcatcgg gttcccgcgc ggcgaagcga
     1141 tcaggatcct cgagggtgag ggcttcgtgg tcgcgacgga ggccgtgccg ctggggagct
     1201 atccgccggg gacggtcgtc gaccagagcc cgtcgggcgg ctcacgcgtc ccggcgggat
     1261 cgacggtcac gctctacatc gcgtcggagg acgtcgagga cgcggcgacg gtcccgggcg
     1321 tgctggggat gacgcggggc gaggccgaga gccggatccg ttcggcaggg ttcgacgtga
     1381 acgtggtcgt cgagagcgag tcgagccgcg gccaggccaa gaagaacgcg ggccgggtgt
     1441 ggaagcaggc gccgggcggg ggggccgagc ttcaggaagg cgacacggtg acgatctggg
     1501 tgaaccccgg ctaggcagtt tcagcggcgg gggcctcagc cgctgtccga ggggacgcgt
     1561 ccgtgccccc ttccacctca ggcgagacct ccctgagcag tgcgttgaag gcgtcgctca
     1621 agagcgcgac gaccttggcc gcgggcaacc ccatcgcagt agcgacttcg ggccctgagc
     1681 ggccagttag caccagtgtc agagcctcga cctgctgagg cgcaaggatg cttcgcagtg
     1741 aacgcgattg ccgaggctcc tcggcagcgg tcatcgagtc gtgcggcccg gacgtcggtt
     1801 tcttctcccc cttttcgtgg ccgacgtcct cgatgagttc ctctagcaat attcgagctg
     1861 tggcccgccg gatatcgtcc cccgcgcgta tcgtgcctgc gcccacggaa gccggtgggg
     1921 gcccaagctt gtgcggcggc gtcagccctg acgtgtatcc gcccttcgag acgggcctcg
     1981 cgcgcatgtc tcggctcatc aggccgccga tccggaatcg ccgaactcca acatctccac
     2041 ttcatcccac ctgatcaaaa ggctcacccc tctaagaata ggttccccgg cggcgagaat
     2101 gaaggctccc gtctcggggt cgacctcgac cgtctccgcg aggaagaggt cttgggcttc
     2161 cggatagcca ctggcgtagg aggggaggcc gacatcgggc ggaccgtaga tccccccgat
     2221 ccaaacccca cgcttcagcc ggagacgcac ccatccgtcc gggcggctgg agaagaagtg
     2281 gtcccaagct cggggagcag gattcggtcc ggtgaacgcc gcggcccacg cctggccctc
     2341 cagggttccg cggccgatca aggtcccagt cgccaacggg accgcgacgt acgcgagcgc
     2401 caggggccaa agaccccagg gcaggg
//
LOCUS       DASQDO010000435         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_352453, whole genome shotgun sequence.
ACCESSION   DASQDO010000435 DASQDO010000000
VERSION     DASQDO010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352453"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1722)
                     /gene="ppk1"
                     /locus_tag="VEV43_12010"
     CDS             complement(<1..>1722)
                     /gene="ppk1"
                     /locus_tag="VEV43_12010"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HYP24289.1"
                     /translation="ACVEHDLRPQLAEHGIRIASMDDLSEDERDELADRFRRQIFPVL
                     TPLAVGLGRPFPYISNLSLSLGVMVHDPIGGQETFARVKVPKEMLTRFMSVGSGHSTF
                     VPLEDLIARHLDQLFPGMEIRYYAMFRVTRDADFTVSDEADDLLRAVDDELRRRRFGE
                     VVRVEVDSGMSARMREGLVEALGAEPQDVFTFDGLLDLNDLMAILKVPGHSGLRDKPW
                     SPITQPRLHAPDEDEPADVMAEMRRGDILVHHPYDSFASSVERLVERAVVDPDVLAIK
                     QTVYRTSDDSPLVPALIRASERGKQAVVLVELKARFDERANMQWARALEQGGVHVVYG
                     HPSLKTHAKCVLIIRREGDGVRHYVHIGTGNYHSKTARLYTDFGLLTCDELIGQDVAD
                     MFNFLTGFGRPRRYRRVLVAPNHLREGIIDEIERTVAAHEEGRSARIALKMNSLVDGR
                     CIRALYRASQAGVQVDLNIRGICCLRPGVPGVSDNIRVTSIVGRFLEHSRIFAFERED
                     DCTVYIGSADLRPRNLDTRVELVVPVLEDSLRGELLDTLERCMADDTNAWTLGEDGAW
                     TRRAPGPE"
BASE COUNT          249 a          586 c          635 g          252 t
ORIGIN      
        1 ctccggcccc ggcgcgcggc gggtccaggc gccgtcctcg ccgagcgtcc acgcgttcgt
       61 gtcgtcggcc atgcagcgct cgagcgtgtc gagcagctcg ccgcgcaggc tgtcctcgag
      121 cacggggacg acgagctcga cgcgggtgtc gaggttgcgc ggcctcaggt cggccgagcc
      181 gatgtagacc gtgcagtcgt cctcgcgctc gaaggcgaag atccgggagt gctcgaggaa
      241 gcgcccgacg atcgaggtca cgcggatgtt gtccgacacg ccgggcacgc cggggcgcag
      301 gcagcagatc ccgcggatgt tgaggtcgac ctggacgccg gcctgggagg cgcggtacag
      361 cgcgcggatg cagcggccgt cgaccagcga gttcatcttc agcgcgatcc gcgcgctgcg
      421 gccctcctcg tgcgcggcca ccgtgcgctc gatctcgtcg atgatcccct cgcgcaggtg
      481 gttgggggcc accagcaccc gccggtagcg ccgcggacgg ccgaagccgg tcaggaagtt
      541 gaacatgtca gcgacgtcct ggccgatcag ctcgtcgcag gtgagcagcc cgaagtccgt
      601 atacagccgc gcggtcttcg agtggtagtt gccggtcccg atgtggacgt agtggcggac
      661 gccgtcgccc tcgcggcgga tgatcagcac gcacttggcg tgggtcttga gcgacgggtg
      721 cccgtagacg acgtggacgc cgccctgctc gagcgcgcgc gcccactgca tgttggcccg
      781 ctcgtcgaag cgcgccttga gctcgaccag gacgaccgcc tgcttgccgc gctcggaggc
      841 gcggatcagc gcgggcacca gcggcgagtc gtcgctcgtg cggtacacgg tctgcttgat
      901 cgccaggacg tcggggtcga ccaccgcccg ctcgaccagc cgctcgaccg agctcgcgaa
      961 cgagtcgtac gggtggtgga cgaggatgtc gccgcggcgc atctcggcca tcacgtcggc
     1021 gggctcgtcc tcgtcgggcg cgtgcagccg cggctgcgtg atcggcgacc acggcttgtc
     1081 gcgcaggccc gagtgcccgg ggaccttgag gatcgccatc aggtcgttga ggtcgagcag
     1141 cccgtcgaac gtgaagacgt cctgcggctc ggcgccgagc gcctcgacca gcccctcgcg
     1201 catccgggcg ctcatccccg agtcgacctc gacgcgcacg acctcgccga accgccggcg
     1261 gcgcagctcg tcgtcgaccg cgcgcaggag gtcgtcggcc tcgtcggaga cggtgaagtc
     1321 ggcgtcgcgc gtgacgcgga acatcgcgta gtagcggatc tccatcccgg ggaacagctg
     1381 gtcgaggtgg cgggcgatga gatcctcgag cgggacgaac gtcgagtggc ccgagccgac
     1441 cgacatgaac cgcgtcagca tctccttggg caccttcacg cgcgcgaagg tctcctgccc
     1501 gccgatcggg tcgtggacca tcaccccgag cgacagcgag aggttggaga tgtaggggaa
     1561 cggccgcccc agcccgacgg ccagcggggt gagcaccggg aagatctggc ggcggaagcg
     1621 gtcggcgagc tcgtcgcgtt cgtcctcgga gagatcgtcc atcgacgcga ttcggatgcc
     1681 gtgctcggcg agctgcgggc gcaggtcgtg ctcgacgcac gc
//
LOCUS       DASQDO010000436         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_169530, whole genome shotgun sequence.
ACCESSION   DASQDO010000436 DASQDO010000000
VERSION     DASQDO010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169530"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VEV43_12015"
     CDS             complement(<1..870)
                     /locus_tag="VEV43_12015"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HYP24290.1"
                     /translation="METPRPRVLVVALGGNALQRPEDDDSVAGDFTRTAETARHLRPL
                     IASGEWRVVVTHGNGPQVGNHLLRSELGHVHGDTPLLPLDVCVADTQGGMGYMIQQCL
                     ANELASADVPAVVTSLVTQVLVDGDDPAFETPSKPVGETVPPDRVAVLEERGWTLVED
                     RRRAGWRRVVASPDPVESVEAEAVRTLVDAGVTVVAGGGGGVPVVQRADGSLEGVAAV
                     VDKDLASALLAADLRAAGLVILTDVAQVALGFGTPEEKPVDRMSVRDARRYLEAGEFP
                     AGSMGPKVEAICRF"
     gene            complement(880..2295)
                     /locus_tag="VEV43_12020"
     CDS             complement(880..2295)
                     /locus_tag="VEV43_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HYP24291.1"
                     /translation="MATSPATRARGLEGYFRITERGSTVRTEVVAGLTTFMTMAYILF
                     LNPIILGGPDRNGDTLAPTAVLTVTALAAGITTIAMGAYANYPFAIATGLGLNAVVAF
                     QLVGGNQLTWPEAMGVVVMEGIIILILVLTKFRQAVMEAVPMPLKQAIAVGIGLFITI
                     IGFVDAGFVRATGIAVPPLQLGTGGELQGWPVTVFVVGLLITIFLFIRNVRGALLIGI
                     LSTTIVAVLVNELFLDGEGFAGKAVLGNPIDFPSGSDFALIGDFSFGFFAEMGLIAAV
                     LAIFTIMLSDFFDTMGTAVGLGREAGLLDEEGRLPDVDKVLVVDSAAAAIGGAFSASS
                     NTTYIESASGIAEGGRTGLTAVVVGGLFLLSILFSPLAEIIPLEAAAPALVVVGFLMM
                     TAVRDIPWDDVDVALPAFLTIVLMPFTYSITNGIGAGFISYVFVKLARGRGNEVAPMM
                     WVAAGAFVVYFAIFYVRDFLT"
     gene            2431..2721
                     /locus_tag="VEV43_12025"
     CDS             2431..2721
                     /locus_tag="VEV43_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24292.1"
                     /translation="MKGTRLLAAAALTAGLMAVAPAASADENCVGAEGTLVVCVDPLG
                     QTIYDTCVYTGGEECTPVSVPGPVITRCDGPPTGDPKAVVAHVIAAACDVIV"
     gene            2758..>3038
                     /locus_tag="VEV43_12030"
     CDS             2758..>3038
                     /locus_tag="VEV43_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24293.1"
                     /translation="MKRLRLFVVAAMAVGSLAVAPAAAAEEACFGEEGTFVLCVEPTG
                     GEVTSVCVYTGGTTCETVTVPGPEVTRCDYYGGGGRPGVYQLVAAAVCNA"
BASE COUNT          498 a         1025 c         1078 g          437 t
ORIGIN      
        1 gaaccggcag atcgcctcga ccttcggccc catgcttccg gccgggaact cgccggcttc
       61 gaggtagcgg cgagcgtcgc gcacgctcat gcggtcgacc ggcttctctt ccggcgtgcc
      121 gaagccgagc gcgacctgcg cgacgtcggt gaggatcacg agacccgcag cgcggaggtc
      181 cgcggccagc aacgccgacg ccaggtcctt gtcgaccacg gcggcgaccc cttcgaggga
      241 tccgtccgct cgctggacga ccgggacgcc accgccccct ccggcgacga cggtcacgcc
      301 cgcgtcgacc aaggtgcgga cggcctcggc ttcgacgctc tcgaccgggt cgggggacgc
      361 gacgactcgg cgccaccccg cgcggcgacg gtcttcgacg agcgtccagc cgcgctcctc
      421 caggacggcg acccgatcgg gggggacggt ctcgcccacc ggcttcgacg gcgtctcgaa
      481 agcggggtcg tcgccgtcca cgagcacctg cgtgaccaag gacgtcacga cggcagggac
      541 gtccgccgac gcgagctcgt tcgccaggca ctgctggatc atgtagccca tgcccccctg
      601 cgtgtcggcg acgcagacgt cgagcggcag caacggcgtg tctccgtgga cgtggccgag
      661 ctcgctgcgc agcaggtggt tgccgacctg ggggccgttc ccgtgcgtga cgaccacgcg
      721 ccactccccc gacgcgatca ggggccggag atggcgcgcc gtctctgcgg tgcgggtgaa
      781 gtctcccgcc acggagtcgt cgtcctccgg ccgctgcaac gcgttgccgc cgagcgcgac
      841 gacgagcaca cggggccggg gtgtctccac cgggtaatgc taggtgagga agtcgcgcac
      901 gtagaagatg gcgaagtaga cgacgaacgc gccggcggcg acccacatca tcggcgccac
      961 ttcgttgcct cgcccccgcg ccagcttgac gaacacgtac gagatgaacc ccgcgccgat
     1021 cccgttcgtg atcgagtagg tgaacggcat cagcacgatc gtcaaaaacg ccgggagcgc
     1081 gacgtcgaca tcgtcccagg ggatgtccct caccgcggtc atcatcaaga agccgacgac
     1141 cacgagggcc ggggccgccg cttcgagcgg gatgatctcg gcgagcggcg agaacaggat
     1201 cgacagcagg aacagccctc cgacgacgac tgcggtgagg ccggtccggc ccccctccgc
     1261 gatgcccgag gcgctctcga tgtaggtcgt gttcgacgac gccgagaagg cgccgccgat
     1321 cgcggctgcc gccgagtcca cgacgaggac cttgtcgacg tcggggagcc ggccctcctc
     1381 gtcgagcagc cccgcctcgc ggccgaggcc gaccgcggtc cccatcgtgt cgaagaagtc
     1441 cgacagcatg atcgtgaaga tcgcgagcac ggccgctatg agccccatct cggcgaagaa
     1501 gccgaacgag aagtccccga tgagggcgaa gtcgctgccc gacgggaagt cgatcgggtt
     1561 gccgagcacc gccttgcccg cgaacccctc gccgtcgagg aacagctcgt tgaccagcac
     1621 ggccacgatc gtcgtgctga ggatcccgat cagcaacgct ccgcgcacgt tgcgtatgaa
     1681 cagaaagatc gtgatcagga ggccgacgac gaagaccgtg acgggccacc cttggagctc
     1741 tcctcccgtc cccagctgca acggagggac ggcgatcccc gtcgccctga cgaatccggc
     1801 gtcgacgaac ccgatgatcg tgatgaacaa cccgatgccg actgcgatcg cctgcttgag
     1861 cggcatcggc accgcctcca tcacggcctg gcggaacttc gtgaggacga ggatcaggat
     1921 gatgatcccc tccatcacca cgacgcccat ggcctcgggc cacgtcagct ggttgccgcc
     1981 gacgagctgg aaggcgacga cggcgttgag tccgagcccc gtggcgatcg cgaacggata
     2041 gttcgcgtaa gcccccatgg cgatcgtcgt gatccctgcg gcgagggcgg tcacggtgag
     2101 cacggcggtc ggggcgagcg tgtcgccgtt gcggtccggg cccccgagga tgatggggtt
     2161 caggaacagg atgtaggcca tggtcatgaa cgtcgtgagg ccggcgacca cctcggtgcg
     2221 aacggtcgag ccccgctcgg tgatcctgaa gtagccctcg agaccccgcg cgcgcgtagc
     2281 cggcgacgtt gccatgtgat ccacccctct cgctcgcacc aacctgggca ggcggattca
     2341 taccagagcg aggtcggggc gcccgatttg acagcagtcc gagcgttttg caccattccc
     2401 tcctcctatc gggcgacgaa aggaagcggg atgaagggga cgcgtctact tgccgcggcc
     2461 gcgctgacgg ccgggttgat ggcggtggcg ccggcggcgt cggcggacga gaactgcgtg
     2521 ggggccgagg ggacgctcgt ggtgtgcgtc gaccctctgg gacagacgat ctacgacacg
     2581 tgtgtctaca cgggcgggga agagtgcacc ccggtctcgg ttccggggcc ggtgatcacg
     2641 cgttgtgatg ggccgcccac cggcgacccg aaggccgtcg tggcgcacgt gatcgcggcc
     2701 gcctgcgacg tgatcgtcta gcgccgtctt gttcgggatc tggcgaaagg ggacgagatg
     2761 aagcggttgc gtctgttcgt ggtggcggcc atggccgtgg gctcgctggc ggtggcgccg
     2821 gcggcggccg cggaggaggc ctgcttcggc gaggaaggga cgttcgtgct gtgcgtcgag
     2881 ccgacgggag gcgaggtcac cagcgtctgc gtctacacgg ggggcacgac gtgcgagacc
     2941 gtcacggtcc ccggccccga ggtgaccagg tgcgactact acgggggcgg cggtcgtccg
     3001 ggcgtgtacc agttggtcgc cgcggcagtg tgcaacgc
//
LOCUS       DASQDO010000437         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_352543, whole genome shotgun sequence.
ACCESSION   DASQDO010000437 DASQDO010000000
VERSION     DASQDO010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352543"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VEV43_12035"
     CDS             <1..217
                     /locus_tag="VEV43_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24294.1"
                     /translation="SDYEEIRIGPVTFQGFPAAEWEYTYSGVHAINIGIVTDTNGYAL
                     NFQTPAEDWEESQALFEAIKESFVPAP"
     gene            311..2167
                     /locus_tag="VEV43_12040"
     CDS             311..2167
                     /locus_tag="VEV43_12040"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HYP24295.1"
                     /translation="MGRISSARIHPAIGIARVGDSPDAFFVGPEVLRPREEDSYKDAE
                     GRLKRQAARFRIYGYDSDGELVGEITSADAEIVWEVHVANTKAAWYDFVVALDLAEAA
                     ALEVPRRNSWMGAGGRGELVIDPGPKTIAGTGQGPAPLDGGSFLGTPVSLGELRTDDA
                     GRLLFLGGHGVSTTPIPGNTLTTFSNNSGWHDDVSDGPVHATVTIGGEEVPVDPAWVV
                     VAPPNYGPGIVTPQTMYDVMVDAVTPMMVPPRKEPSFTADILPLLEQFPDSQWVNAGF
                     LAQFGWGAPNEFTRPEILRKLASPDVTHQELRRSIFYMFRQPGNAVPNDVQWPPMYGD
                     AFGNYDNSAKAGFTITKTLYGYLNSWMNGSFVADYDPDAPRPETLDDVDPAERPAMLD
                     RAALHWCMGGPFHPGCEMTWPMRQIAMYRAPFRLKERPPGTPRPDYGDYLSPDVALAD
                     DGPLSQSGPGDVTRWMAVPWQTDTASCRSGYPRPPFPTDPYLPTFWPSRVPNEVLTED
                     SYAIVVDPDQPMENREEAFNTRLSWLRGLDLNAPYIHQITKMISDFGKLGVVERREAP
                     GDGPFPPVMYVETGMTIDMHPRVRAAAEEARGTERVSEEFRQARYGFPGRRI"
     gene            2181..3191
                     /locus_tag="VEV43_12045"
     CDS             2181..3191
                     /locus_tag="VEV43_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24296.1"
                     /translation="MLLRQATEATSCDCATDPIACLKYLFVDVTQKTRMLAGQCPVRR
                     AVFLKQHGCAYGEFVVQPDLPEDLRVGVFAGDRYPMWVRSSSDTVPGNADTGTTLGMG
                     VKLFDVPGEKLLEPDALTQDFVFQNFDVFFVPDAHAMCEFTYAGVQEHDYPSYLKAHP
                     KTARILNDMSQKVGSVTSIDYWSVLPSKFGPDRFVKYKLEPVLDGPPPPPPASPSQDY
                     LADDLKARLLAGPVKFRFLVQFQKDAESMPLDDATVRWDESASRPVHVATIELPQQDV
                     DAPGQEEFGENLSFTAWHTLEAHEPVGSLQDARKVVYQASATLRRNANGIPLVEPRSP
                     RP"
     gene            3196..4257
                     /locus_tag="VEV43_12050"
     CDS             3196..4257
                     /locus_tag="VEV43_12050"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HYP24297.1"
                     /translation="MEVDVAVVGGGPAGAVAARCLASWGRRVAVFERSRYSEPRYGET
                     LPPETMPLLDALGLREELAHVEAAESPGTVSSWGGDPVETDFVRNVHGAGLHVDRSGF
                     DRALARAAEDAGASLSTGVTVTDVAVVGGAWRLTTSRGPATARVVVDAAGRLGHVIPR
                     RRTAYADKLLAVTALLRHRRARPSDLRTYVETTPRGWWYSAPVGASRTVAMFFTDGVE
                     HRRGISPPGELDGAPLTRARMAGAEVVDERVLPVRSGLRGPVAGPGWVAVGDSAASYD
                     PVSGAGIHKALGQGALAAAAVHAALEGDSAAQLLYEERVRADFERYAAERHLVYSAER
                     RWAGAGFWERRTRAGGRTA"
     gene            4286..>4655
                     /locus_tag="VEV43_12055"
     CDS             4286..>4655
                     /locus_tag="VEV43_12055"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HYP24298.1"
                     /translation="MDKARDRMRRTRLSERGTGGSKPVPPRGSGQQGGPDQQRWMTVA
                     WIVGGLVLVMLFQSVFGPQTEEISFTRLFELAEDGKIAEASVSETSVSGTIEERGEDP
                     RQLTTTIPPNYVANAIVGRLE"
BASE COUNT          713 a         1641 c         1638 g          663 t
ORIGIN      
        1 ctcggactac gaggagatcc gcatcggccc ggtcacgttc cagggcttcc cggcggccga
       61 gtgggagtac acctactccg gcgtgcacgc gatcaacatc ggcatagtca ccgacaccaa
      121 cggctacgcg ctgaacttcc agaccccggc cgaagactgg gaagagtccc aagccctctt
      181 cgaggcgata aaagagtcct tcgtcccggc cccctagagg tctaccggat cgggggagcg
      241 cacccccctt gtaacagcga tggggctcct gttacatagc ggcgtcaacc gagtgacgct
      301 agggggcaaa gtggggcgca tctccagtgc tcgtatccat ccggccatcg gcatcgcacg
      361 cgtgggcgac agccctgacg cgttcttcgt cggccccgag gtcctgcgcc cgcgcgagga
      421 ggactcctac aaggacgccg agggccggct caagcgccag gcggctcgct tccgcatcta
      481 cggctacgac tccgacggcg agctcgtcgg cgagatcacc tccgcggacg ccgagatcgt
      541 gtgggaggtc cacgtcgcga acaccaaggc ggcctggtac gacttcgtcg tcgcgctcga
      601 cctggcggag gccgcggccc tcgaggtacc ccggcggaac tcgtggatgg gcgcgggcgg
      661 ccgcggcgag ctcgtgatcg accccggtcc gaagacgatc gcgggaaccg gccagggtcc
      721 ggcgccgctc gacgggggct cgttcctcgg cacgccggtg tcgctgggag agctgaggac
      781 cgacgacgcc ggccgcctgc tgttcctcgg gggacacggt gtctccacca cgcccatccc
      841 aggcaacacg ctcacgacgt tctcgaacaa ctccggctgg cacgacgacg tgtccgacgg
      901 gccggtgcac gcgaccgtga cgatcggcgg cgaggaggtt cccgtcgacc cggcgtgggt
      961 cgtcgtcgca ccgccgaact acggtcccgg gatcgtcacg ccgcagacga tgtacgacgt
     1021 catggtcgac gcggtcacgc ccatgatggt gccgccccgg aaagagccgt ccttcacggc
     1081 cgacatcctg ccgctgctcg agcagttccc cgactcgcag tgggtgaacg ccgggttcct
     1141 cgcccagttc gggtggggcg cgccgaacga gttcacgcgg ccggagatcc ttcgcaagct
     1201 cgcgtcgccg gacgtcacgc accaggagct acgcaggtcg atcttctaca tgttcagaca
     1261 gcccggcaac gcggtgccca acgacgtgca gtggccgccg atgtacgggg acgcgttcgg
     1321 gaactacgac aacagcgcca aggccggctt cacgatcacg aagactttgt acggatacct
     1381 gaacagctgg atgaacggct cgttcgtcgc cgactacgac ccggacgccc cgcggccgga
     1441 gacgctcgac gacgtcgatc ccgccgagcg ccccgccatg ctcgaccgcg ccgcgctcca
     1501 ctggtgcatg ggggggccgt tccacccggg ctgcgagatg acgtggccga tgcggcagat
     1561 cgccatgtac cgcgcgccgt tccggctcaa ggagaggccc ccgggcacgc cgcgccccga
     1621 ctacggcgac tacctctccc cggacgtcgc acttgccgac gacggcccgc tgtcgcagag
     1681 cgggcccggc gacgtgacga ggtggatggc ggtgccgtgg cagacggaca ccgcgagctg
     1741 caggtccggc taccccagac cgccgttccc gaccgacccg tacctgccga cgttctggcc
     1801 ctcgcgcgtg ccgaacgagg tgctgaccga ggacagctac gcgatcgtcg tcgacccgga
     1861 ccagccgatg gagaaccggg aggaggcgtt caacacgagg ctgtcgtggc tgcgcggcct
     1921 cgacctgaac gctccgtaca tccatcagat cacgaagatg atctcggact tcgggaagct
     1981 cggcgtcgtc gaacgacgcg aggcccccgg cgacggcccg ttcccgccgg tgatgtacgt
     2041 cgagaccggg atgacgatcg acatgcaccc gcgcgttcgc gccgccgccg aagaggcacg
     2101 agggaccgag cgggtctcgg aggagttccg ccaggcgcgc tacggcttcc ccggccgccg
     2161 gatctgagag gagaaggcag atgctgctgc gccaggcaac cgaggccacg agctgcgact
     2221 gcgcgaccga tccgatcgcg tgcctgaagt acctgttcgt cgacgtcacg cagaagacgc
     2281 ggatgctcgc ggggcagtgc cccgtgcgac gcgcggtctt cctgaagcag cacggctgcg
     2341 cctacggcga gttcgtggtg cagccggacc tcccggaaga cctccgcgtc ggcgtcttcg
     2401 ccggagaccg ctatccgatg tgggtgcgct cctccagcga caccgtcccc ggcaacgccg
     2461 acaccgggac gacgctcggg atgggcgtca agctcttcga cgtccccggc gagaagctgc
     2521 tcgagccgga cgcgctcacg caggacttcg tcttccagaa cttcgacgtg ttcttcgtgc
     2581 cggacgcaca cgcgatgtgc gagttcacct acgcgggcgt gcaggagcac gactatccct
     2641 cctacctgaa ggcgcacccg aagaccgctc ggatcctgaa cgacatgtcg cagaaggtcg
     2701 gcagcgtcac gtcgatcgac tactggagcg tcttaccgtc gaagttcggg ccggaccgct
     2761 tcgtgaagta caagctcgag cccgtcctcg acggccctcc tccgccacct ccggcgtcgc
     2821 cgtcgcagga ctacctggcg gacgacctca aggcgcggct gctcgcgggc ccggtgaagt
     2881 tccggttcct cgtccagttc cagaaggacg ccgagtcgat gccgctggac gacgcgaccg
     2941 tccggtggga cgagtcggcg tcgaggccgg tgcacgtcgc cacgatcgag ctgccgcagc
     3001 aggacgtcga cgcgccggga caggaggagt tcggcgagaa cctgtcgttc accgcgtggc
     3061 acacactcga ggcgcacgag ccggtcggga gcctgcagga cgcgcgcaag gtcgtgtatc
     3121 aagcttctgc cacgctcagg cgcaacgcga acggcatccc gctcgtagag ccccggtccc
     3181 cgcgtccgtg acgtagtgga ggtcgacgtc gccgtcgtcg gcggcggccc cgccggagcc
     3241 gtcgccgcgc gctgcctcgc gtcgtggggc cggcgcgtcg cggtgttcga gaggtcgcgc
     3301 tactccgagc cgcgctacgg cgagacgctt ccaccggaga cgatgccgct cctggacgcg
     3361 ctcggccttc gcgaggagct cgcgcacgtc gaggcggccg agtcgccggg aacggtgtcc
     3421 tcgtggggcg gcgatcccgt cgagaccgac ttcgtgcgga acgtccacgg cgcgggcctg
     3481 cacgtcgatc ggtcgggctt cgaccgtgcg ctcgcgcgcg cggccgagga cgcgggggcg
     3541 tcgttgtcga cgggtgtcac ggtgacggac gtcgccgtcg tcggcggcgc gtggcggctg
     3601 acgacgagcc gggggccggc cacggcgcgg gtagtggtcg acgctgccgg tcgtctcggt
     3661 cacgtgatcc cgcgccggcg gaccgcgtac gccgacaagc tcctcgccgt gacggccctc
     3721 ctccgtcacc ggcgcgcgcg gccctcggat ctccggacgt acgtcgagac cacgccgcgc
     3781 ggctggtggt attcggcccc cgtgggggcg agccggaccg tggcgatgtt cttcaccgac
     3841 ggggtcgagc accgccgcgg catctcgccg ccgggggagc tcgacggcgc gcccctgacc
     3901 cgagctcgga tggccggcgc cgaggtcgtc gacgaacgcg tgctcccggt ccgctcgggg
     3961 ttgcgcggcc cggtcgcggg gcccggttgg gtggccgtgg gggacagcgc cgccagctac
     4021 gaccccgtct cgggagcggg catccacaag gcgctcgggc agggggcgct cgcggccgct
     4081 gcggtgcacg ccgcgctcga gggtgactcc gctgcccagc tgctctacga agagcgcgtc
     4141 cgggcggact tcgagcgcta tgccgccgag cgtcacctcg tctattcggc ggagcggcgc
     4201 tgggccggcg cgggtttctg ggagcggcga acgcgggccg ggggcagaac ggcctaggga
     4261 ccctcgccta taggctcgcc cggtgatgga caaggcgcgc gaccgcatgc ggcgcacacg
     4321 gctctccgag cgcggcaccg gcggctccaa gcccgtgccc ccgcgcggct ccggccagca
     4381 gggcggcccg gaccagcagc ggtggatgac cgtcgcctgg atcgtcggcg ggctcgtgct
     4441 cgtcatgctc ttccagagcg tcttcggccc ccagaccgag gagatctcgt tcacgcggct
     4501 gttcgagctc gccgaggacg ggaagatcgc cgaggccagc gtctccgaga ccagcgtctc
     4561 gggcactatc gaggagcgcg gcgaggaccc gcgtcagttg acgacgacga tcccgccgaa
     4621 ctacgtggcg aacgcgatcg tcggccggct cgaga
//
LOCUS       DASQDO010000438         6202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1209406, whole genome shotgun sequence.
ACCESSION   DASQDO010000438 DASQDO010000000
VERSION     DASQDO010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209406"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..581
                     /locus_tag="VEV43_12060"
     CDS             27..581
                     /locus_tag="VEV43_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYP24299.1"
                     /translation="MSGKLLYTAICSLDGYVADEEGRFDRLAPDAEVHAFVNDLERRV
                     GTYLYGRRMYEVMVYWETPTADSPEVELDYARVWQAADKIVYSATLADVSSTRTTLER
                     SFDPGRVAELKASATADLSVSGPNLAAQALAAGLVEEVALLLAPVTLGGGNPVLPAGW
                     RARLELLDDRRFGNGFAYLRYAVT"
     gene            complement(574..1221)
                     /locus_tag="VEV43_12065"
     CDS             complement(574..1221)
                     /locus_tag="VEV43_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HYP24300.1"
                     /translation="MKVEVWADVVCPWCYIGKVRFERALASFPGRDDVEVVYRAFELD
                     PTIPRGETRDVVTELASKKGLSPEQVRRMMSGVEELAAAEGLEYHLAEAPAGNTVDAH
                     RLVHLAADRGRAGEMVERLYRAHFTELRSIFDLGSLATLAGEAGLDEYEARRVLEKDD
                     YLDRVRGDEAVARSLGIGGVPFFVIDGRFGVSGAQPAEVFEQALERASNREPSAR"
     gene            complement(1650..2147)
                     /locus_tag="VEV43_12070"
     CDS             complement(1650..2147)
                     /locus_tag="VEV43_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYP24301.1"
                     /translation="MVAATSSGSAKLTLPADDQILITREFAAPRDLVWKACTTPELVR
                     KWWGAEMGEVTVAEIDFRVGGTWRYVMVATGGFEVAFHGEYREIVPVERIVSTEVYEG
                     APDADENGTVNTVTYEEIDGGTKMTVLVQCPSPEVRDAIIESGMEGGMQKSYDALERV
                     AQSLA"
     gene            complement(2131..2484)
                     /locus_tag="VEV43_12075"
     CDS             complement(2131..2484)
                     /locus_tag="VEV43_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYP24302.1"
                     /translation="MARAATTTDAFNAVAEPRRRQILDALAGGERSVNDLVAALGIAQ
                     PQVSKHLKVLREVGLVAVREEGRRRMYRLEGRPLKPIHDWIKNYERMWNERFDRLDQV
                     LEEMQEQEERHGGSN"
     gene            2561..4042
                     /locus_tag="VEV43_12080"
     CDS             2561..4042
                     /locus_tag="VEV43_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HYP24303.1"
                     /translation="MPTRPEAMPALDVDPKHVRALIDRAGDLVHEFYERLPSLPVDRR
                     RTIEEVRALVARPIPEEGLGDDELFAYLRDVVFETAMYPGHPAFLAYVVGAGTPHGAV
                     ADLVAGAINQNVGGWRLGPGATEMELHLTSWFARAFGMPEGAGGLVTSGGSMANFVGL
                     KVARDRALGLDVRVDGIGGARLTAYTSGEVHFATTRAADMLGLGSAAVRRIPFDDRYK
                     VDMAELREAIRRDRADGFTPFAVVATAGTVGTGAVDPLAEIADLCEAEGLWMHVDAAY
                     GGPAAFSDELRPLFEGIERAHSIAFDPHKWLYTPQSGGVVLVRDLQWLSDSFAAHATY
                     VHEAKELTGRGIDFGMMGPQLSRSFWALKVMVSLLAYGRRAYSERIAHDVALARYLAE
                     LVEEHDELELMAPVELSICCFRYVPRGYDADDEYLDMLNERLMAAVQSDGRVYFSNAV
                     LHDRFVLRCCIVNFRTEAEQLEKVVAVTVELGAKLDAEMRPLR"
     gene            4039..4923
                     /locus_tag="VEV43_12085"
     CDS             4039..4923
                     /locus_tag="VEV43_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYP24304.1"
                     /translation="MTGGAPDRVTLGAFAGAVAIGGSNFIAVKLSNDELEPLFGAGLR
                     FSAAALLLVVLMRVQRLAVPRGPDLAGPALYGLLGFGVSYGLLYYALQGLTAGTAAVF
                     MAATPLVTLVLAVLHGQERFTPRGVAGGLLAVGGLAVISAESIGGDLRPSYLVAALLG
                     VVAVAESSVLVKGFPRAHPITTNAVGMVAGALLLIVASLVFGEAWELPQSAATWGAVV
                     WLVIPGSIGLFALFVFVIKRWTASATVYALTLMPVVAITLGAVVKDEPVTAELLGGGA
                     LVMAAVYVGALSGRSGAV"
     gene            4953..5930
                     /locus_tag="VEV43_12090"
     CDS             4953..5930
                     /locus_tag="VEV43_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYP24305.1"
                     /translation="MNLLSRLAALFLVFKRMKAKRGLPPDPPPPDRPAADRPSAAPAT
                     PSGQASTAPETPVQLEPADWKVTAKRTLKEIKDDRVTLAAAGMAYYFFLALFPAMIAV
                     IGIMDLANVSTAAFVDTVRDTLPGGTGQLVVDAVQGRDPDEGRSLTAAITGVALALWS
                     ASSGMAALQSGLNVAYDVPEDRKFVGKRLVSFALLLAAGILGGVPTPFVAGEGAIQYV
                     GWALTIPAVVVLFAFFYYLGPKRESPKWVWVTPGGLVGALLWLVVSGAFGFYVKEFAT
                     YGKTYGSLASVIVLIFWLDLSALTILVGGELNAEVERQAARRGSDPQAS"
     gene            complement(5932..>6202)
                     /locus_tag="VEV43_12095"
     CDS             complement(5932..>6202)
                     /locus_tag="VEV43_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24306.1"
                     /translation="MNDLPLLESVGHPHAVNPEPELRRIARVRGWHVHELRTRRRALL
                     IGIPAGLGGAGLFASGVAAGSWAVRRRAARKQRGPVERLGRRFRI"
BASE COUNT          834 a         2161 c         2192 g         1015 t
ORIGIN      
        1 aggatcgact cgcgggtcgg agagagttga gcggcaagct cctctacacc gcgatctgct
       61 cgctcgacgg atacgtcgcc gacgaagagg gccgcttcga ccggctcgct cccgacgcgg
      121 aggtacatgc gttcgtcaac gacctcgaac ggcgcgtcgg cacgtatctc tacggccgcc
      181 gcatgtacga ggtgatggtc tattgggaga ccccgaccgc ggattcgccg gaggtcgagc
      241 tcgactacgc gcgggtctgg caggccgccg acaagatcgt gtattcggcg acgctggcgg
      301 acgtctcgag cacgaggacg accttggagc gatcctttga cccgggccgc gtcgcggagc
      361 tcaaggcctc ggcgacggcg gacctgtcgg tgagcgggcc gaatctcgcc gcgcaggcac
      421 ttgctgcggg gctcgtcgag gaggtcgcgc tgctcctcgc acccgtcacc ctcgggggag
      481 gcaaccccgt gctcccggcc gggtggcgtg cgcgcctcga gctactcgac gaccgccgct
      541 tcggcaacgg cttcgcctac ctgcgctacg cggtcaccta gccgacggct cgcggttcga
      601 cgccctttcg agggcctgct cgaagacctc ggccggctgc gcgcccgaga ccccgaagcg
      661 cccgtcgatc acgaagaacg ggacgccgcc gatcccgagc gaccgggcta ccgcctcgtc
      721 gccgcggact ctgtcgaggt agtcgtcctt ttccaggacg cggcgcgctt cgtactcgtc
      781 cagtcccgcc tcgcctgcca gcgtcgcgag ggagccgagg tcgaagatcg aacggagctc
      841 ggtgaagtgg gcccggtaca gccgctcgac catctcgccg gcacgcccgc ggtcggcggc
      901 gaggtgcacg agccggtggg cgtcgacggt gttgccggcg ggggcctcgg cgaggtggta
      961 ctcgagcccc tctgcggcgg cgagctcctc gacgccggac atcatgcggc ggacctgctc
     1021 ggggctcagg cccttcttcg acgcgagctc ggtgacgacg tcacgcgtct cgccgcgcgg
     1081 gatcgtgggg tcgagctcga aggcgcggta gacgacctcg acgtcgtcgc gcccggggaa
     1141 ggaggcgagc gcgcgctcga acctgacctt gccgatgtag caccagggac agacgacgtc
     1201 cgcccagacc tcgaccttca tgagcccgct ccgttgctag acgaccctct cgcctcgtgc
     1261 aacgcgttct ccgggcgggg tcttccccgc tacgtcgagg atggacgatg attacgctcg
     1321 tggaaggagt gacagccgac gcggagggac ctgggaagag ggctttggcc gacgagatcg
     1381 atccggccgc atcggtggag gacggcggtc ccgctgttcg gcccccgggc tggggcacaa
     1441 ctcggagcgg acgaccgatg ccggattcgg tcgctctgat cctcgagcta cgcgatggcg
     1501 tcgaagcccg accggcgcgg gaacgtcagg cgtagccgaa gcccgttcga ccgtctctcg
     1561 gccccctatc gcttctccat gcgcgtgttc ctacaccgcg aacaggcccc gggctgaccc
     1621 ggggcctgtt cgactggacc gagatctcgc taggcgagcg actgcgcgac ccgctcgagg
     1681 gcgtcgtacg acttctgcat cccgccctcc atgccggact cgatgatcgc gtcgcggacc
     1741 tcggggctcg ggcactggac gaggaccgtc atcttcgtcc cgccgtcgat ctcctcgtag
     1801 gtgaccgtgt tcacggtgcc gttctcgtcc gcgtccgggg ccccctcgta cacctcggtc
     1861 gagacgatcc gctcgaccgg gacgatctcg cggtactcgc cgtggaacgc gacctcgaag
     1921 cccccggtcg cgaccatcac gtagcgccac gtgcctccga cacggaagtc gatctcggcg
     1981 accgtgacct cgcccatctc cgcgccccac cacttgcgga cgagctcggg cgtcgtgcat
     2041 gccttccaca cgaggtcgcg gggggccgcg aactcccggg tgatcaggat ctggtcgtcg
     2101 gccggaagcg tgagcttcgc actgccacta ctagttgctg ccaccatgtc gctcctcctg
     2161 ttcctgcatc tcttccaaca cctggtccaa ccggtcgaag cgttcgttcc acatgcgctc
     2221 gtagttcttg atccagtcgt ggatcggctt gagcggtcgg ccctcgagcc ggtacatccg
     2281 gcggcggccc tcctctcgca cggcgacgag cccgacctcg cgcagcacct tgaggtgctt
     2341 cgagacctgc ggctgcgcga tgccgagggc ggcgacgagg tcgttcaccg accgctcgcc
     2401 accggcgagg gcgtcgagga tctgccgccg ccggggctcc gccactgcgt tgaaggcgtc
     2461 agtcgtggtc gctgctcttg ccatgggacg gatcatatgc cgatatagga atatgtcaag
     2521 agcgatctcc agcaaggcga cctcgtggtg tgattgccgg atgccgacac gaccggaagc
     2581 gatgcccgcc ctcgacgtcg acccgaagca cgtccgcgcg ctgatcgacc gcgccggaga
     2641 cctggtccac gagttctacg aacgtctccc gtcgctccct gtcgaccgca ggcgcacgat
     2701 cgaggaggtg agggccctcg tcgcgcggcc gatcccggag gaggggctcg gcgacgacga
     2761 gctgttcgcc tacctgcgcg acgtcgtgtt cgagaccgcg atgtacccgg gacatcccgc
     2821 gttcctcgcg tacgtcgtgg gggccgggac cccccacggt gcggtcgccg acctagtggc
     2881 cggggcgatc aaccagaacg tgggcggatg gcggctcggc cccggcgcga ccgagatgga
     2941 gctgcacctg acgtcgtggt tcgcacgcgc cttcggtatg cccgagggcg ccggcgggct
     3001 cgtgacgtcc ggagggtcga tggcgaactt cgtcgggctg aaggtggcgc gcgaccgcgc
     3061 gctcgggctc gacgtccgtg tcgacgggat cggcggtgcg cgcctcaccg cgtacacgag
     3121 cggcgaggtc cacttcgcga cgacgcgggc cgcggacatg ctcggtctcg gctcggctgc
     3181 cgtgcgccgg atcccgttcg acgaccgcta caaggtcgac atggccgagc tgagagaggc
     3241 gatccgccgc gaccgcgctg acgggttcac tccgttcgcc gtcgtcgcga ctgctgggac
     3301 ggtcggtacc ggcgcggtcg acccgctcgc cgagatcgcc gacctgtgcg aggccgaggg
     3361 gctgtggatg cacgtcgacg cggcctacgg gggcccggcc gccttctccg acgagctccg
     3421 gccgttgttc gagggcatcg aacgtgcgca ctcgatcgcg ttcgacccgc acaagtggct
     3481 ctacacgccg cagtccggcg gcgtcgtgct cgtgcgcgac ctgcaatggc tgtcggactc
     3541 gttcgcggcg cacgcgacct acgtccacga ggccaaggag ctcaccggcc gcgggatcga
     3601 cttcgggatg atggggccgc agctgtcgcg cagcttctgg gcgctgaagg tcatggtgtc
     3661 gctgctcgcg tacgggcgac gcgcgtactc ggagcggatc gcacacgacg tcgcgctcgc
     3721 tcggtacctc gcggagctgg tcgaggagca cgacgagctg gagctcatgg ccccggtcga
     3781 gctctcgatc tgctgcttcc ggtacgtccc gcgcggctac gacgccgacg acgagtacct
     3841 cgacatgctc aacgagcggc tcatggctgc ggtgcagtcc gacgggcgcg tgtacttctc
     3901 gaacgccgtc ctgcacgatc gcttcgtcct gcgatgctgc atcgtgaact tccggacgga
     3961 ggcggagcag ctcgagaagg tggtggcggt gaccgtggag ctgggcgcga agctggacgc
     4021 ggagatgcgc ccgctgcgat gaccggcggg gcccccgacc gcgtgacgct gggggcgttc
     4081 gccggcgcgg tcgcgatcgg cgggtcgaac ttcatcgcgg tgaagctcag caacgacgag
     4141 ctcgagccgc tgttcggtgc cggcctccgc ttctccgctg cggccctgtt gctcgtcgtg
     4201 ttgatgcgcg tgcagcgtct cgcggtgccg cgggggcccg acctcgccgg cccggccctc
     4261 tacggactcc tcgggttcgg cgtctcgtac ggcctcctgt actacgcgct gcaggggctg
     4321 acggcgggaa cggccgcggt gttcatggcc gcgaccccac tggtcacgct cgtcctcgcc
     4381 gtcctccacg gacaggagcg gttcacgccc cggggcgtcg ccggcgggct gctcgcggtg
     4441 gggggcctcg ccgtgatctc ggccgagtcc atcggcggcg acctccgccc gtcgtatctg
     4501 gtcgcggcgc tgctcggcgt cgtcgccgtc gcggagtcga gcgtgctggt gaagggcttc
     4561 ccgcgcgcgc atccgataac gacgaacgcg gtggggatgg tcgccggcgc gctgctgctg
     4621 atcgtcgctt cgctggtctt cggcgaagcc tgggagctcc cgcagagcgc cgcgacctgg
     4681 ggcgcggtcg tgtggctggt gatccccggc tcgatcgggc tgttcgcgct gttcgtcttc
     4741 gtgatcaaga gatggacggc ctcggcgacg gtctacgcgc tgacgctgat gccggtcgtc
     4801 gcgataacgc tcggggccgt ggtgaaggac gagccggtca cggccgagct gctcggcggc
     4861 ggggcgctgg tgatggcggc cgtgtacgta ggtgcactgt cgggccggtc gggcgccgtc
     4921 taggacgcgg tcgcctgggt atgtaggcgc ccgtgaacct gctctcgcgc ctcgccgcgc
     4981 tcttcctcgt cttcaagcgg atgaaggcca agcgcggcct cccgcccgac cctccgcccc
     5041 cggaccgccc ggccgcggac cggccttccg cggcccccgc gacgccgtcg ggccaggcga
     5101 gcacggcgcc ggagacaccc gtccagctcg agcccgccga ctggaaggtc acggccaagc
     5161 gaacgctgaa ggagatcaag gacgaccgcg tgacgcttgc ggccgccggc atggcctact
     5221 acttcttcct cgcgttgttc ccggccatga tcgcggtgat cggcatcatg gacctggcca
     5281 acgtgagcac ggcggcgttc gtcgacaccg tgcgcgacac gctgcccggg gggacgggcc
     5341 agctcgtcgt cgacgcggtg caggggcgcg atcccgacga aggccgttcg ctcaccgcgg
     5401 ccatcaccgg tgtcgccctc gctctgtgga gcgcgtcgtc gggcatggcc gcgttgcagt
     5461 ccggtctcaa cgtcgcgtac gacgtccccg aagaccgcaa gttcgtaggc aagcgtctcg
     5521 tctcgttcgc gctgctgctc gcagcaggga tcctcggcgg cgtgccgacg ccgttcgtcg
     5581 caggtgaggg cgcgatccag tacgtcgggt gggcgctgac gatccccgcg gtggtcgtgc
     5641 tgttcgcgtt cttctactac ctcggtccta agcgcgagtc gccgaagtgg gtgtgggtga
     5701 cgcccggcgg gctggtgggc gctctgctgt ggctcgtcgt cagcggcgcg ttcggcttct
     5761 acgtcaagga gttcgccacc tacggaaaga cctacggctc gctggcgagc gtgatcgtgc
     5821 tgatcttctg gctcgacctg agcgcgctga cgatcctcgt cggcggcgag ctgaacgccg
     5881 aggtcgagcg ccaggcggcg cggcgcggga gcgacccgca ggcgtcctga ctcagatcct
     5941 gaacctgcgg ccgagcctct ccaccggccc ccgctgtttc ctcgccgctc gccggcgcac
     6001 cgcccagcta ccggcggcca cccccgaggc gaagaggccc gcgcccccga gccccgccgg
     6061 gatcccgatg agcagggccc ggcgccgggt gcgcagctcg tggacgtgcc agccgcgcac
     6121 gcgcgcgatc cggcgtagct cgggctcggg gttcaccgcg tgcgggtggc cgaccgactc
     6181 gagcagcggg aggtcgttca tc
//
LOCUS       DASQDO010000439         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_230527, whole genome shotgun sequence.
ACCESSION   DASQDO010000439 DASQDO010000000
VERSION     DASQDO010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230527"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VEV43_12100"
     CDS             complement(<1..260)
                     /locus_tag="VEV43_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24307.1"
                     /translation="MPGGRHSSPDQLHFYKSVVAWFLPWVLIAVVVGTAVWIAVEAVS
                     GATESSAPAAASTPTAEPTDEPEPTEKPATAAPEKTAKPSAEP"
     gene            complement(270..563)
                     /locus_tag="VEV43_12105"
     CDS             complement(270..563)
                     /locus_tag="VEV43_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HYP24308.1"
                     /translation="MDEQTSGQAADLSERDVAILDFERSWWKHAGVKEQAIRERFDMS
                     ATRYYQLLNELLENPAALAHDPILVKRLKRLRTYRQRQRVARLLGAEYSPGRK"
     gene            731..1108
                     /locus_tag="VEV43_12110"
     CDS             731..1108
                     /locus_tag="VEV43_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24309.1"
                     /translation="MLRRSRALAATVMAVVALAVPAPASAFDAADPVVAHCRADVGFS
                     SDEWYQTVYVTGAMAPVGATHVWLTCGVVRHGETVANVGEDVDFSAAVVAGTATVLAG
                     PISICYEATVLYSERYEYFDTCP"
     gene            1134..1514
                     /locus_tag="VEV43_12115"
     CDS             1134..1514
                     /locus_tag="VEV43_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24310.1"
                     /translation="MSKLKRVLAVTAAAAAIAGAVPVTAGASAEVAVGPCQARSLVQG
                     VKEWYQEVLVTGEAHVAGAIDVALTCGVVRYGETVDRVSEQRPGPVAVLADVVSVHAS
                     SSISSCYTLRVTYIDHSTYKNTCP"
BASE COUNT          248 a          560 c          574 g          249 t
ORIGIN      
        1 ggctcggcgg agggcttcgc cgtcttctca ggcgctgcgg tggcgggctt ctccgtcggc
       61 tcgggctcgt ccgtaggctc ggccgtgggg gtcgaggctg ccgccggcgc cgagctctcg
      121 gtcgcgccgc tgacggcctc gaccgcgatc cacaccgccg tccccacgac gaccgcgatg
      181 agaacccacg gcaggaacca cgcgacgacg gacttgtaga agtgcagctg gtcgggcgag
      241 gagtgacgac ccccgggcac ggcggggtcc tacttcctgc ccggcgagta ctccgctccg
      301 agaaggcggg cgacgcgctg cctctgccgg taggtgcgaa gcctcttgag acgcttgacg
      361 aggatcgggt cgtgcgccag cgcggcaggg ttctcgagga gctcgttcaa gagctggtag
      421 tagcgcgtcg cggacatgtc gaagcgctcg cggatcgcct gctccttcac gccggcgtgc
      481 ttccaccagg agcgctcgaa gtcgaggatc gcgacgtcgc gctcggagag gtcggcggct
      541 tgcccggaag tctgctcgtc catacgcaca gcctatggcg agggttttcg cgaggcgcgg
      601 attttccgcg ttccggtgac cttgaccgat cgcttcacac gagcacgact atttctcaac
      661 cgcgccggaa ggggaacgag cggagcgggt cgaaggaacc accgcccgac cccgaccgag
      721 gaggttgccg ttgctacgaa gatccagggc cctggccgcg accgtgatgg cggtggtggc
      781 cctagccgtt cccgcgcccg cctccgcctt cgacgcggcg gaccccgtgg tcgcccactg
      841 ccgggccgac gttgggttct cgtcggacga gtggtaccag acggtctacg tgacgggagc
      901 gatggccccg gtcggcgcga cccacgtctg gctgacgtgc ggcgtcgtcc gccacggcga
      961 gaccgtggcc aacgtcggcg aggacgtcga cttctcggcc gcggtcgtcg caggcaccgc
     1021 gaccgtcctg gccggcccga tcagcatctg ctacgaggcg acggtcctct acagcgagcg
     1081 ctacgagtac ttcgacacct gcccgtagaa gagcccgaga gaaaggcgaa cgcatgagca
     1141 agctgaagcg cgtcctcgct gtgacggcgg ccgcggcggc catcgccggt gccgtccccg
     1201 tgaccgcggg ggccagtgcc gaagtagcgg tcggaccgtg tcaggcacgg tcactcgtgc
     1261 agggcgtcaa ggagtggtac caggaggtcc tcgtgacggg cgaggcccat gtggccggcg
     1321 ccatcgacgt cgccctcact tgcggcgtcg tccgctacgg cgagacggtc gaccgtgtga
     1381 gcgagcagcg ccccggcccc gtcgcagtcc tcgccgacgt cgtgagcgtc cacgcctcgt
     1441 cgtcgatcag cagctgctac accctccgcg tcacgtacat cgaccacagc acctacaaga
     1501 acacctgccc gtagaggagg agcacgatgc gtagattcaa gactctcgtc gccgccgtcg
     1561 tcacggcagc agggctcgtg ccggtcggcg cgacgcccgc ccacgcgatg gccgcgtgcc
     1621 acgtgagggt g
//
LOCUS       DASQDO010000440         1948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_842309, whole genome shotgun sequence.
ACCESSION   DASQDO010000440 DASQDO010000000
VERSION     DASQDO010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842309"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VEV43_12120"
     CDS             <1..665
                     /locus_tag="VEV43_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24311.1"
                     /translation="AEPDATEAPAAAPEPACDNQEAVLGDPSLRAGSPEEADVDGDGT
                     PETVSVYYDPEGQVGCEAFVAAEGDETTVAGALETWRGEFGLPMPTLHSVADVDGEPG
                     DEIVVNMGGGASTQFVGLVVADGAVLRQVTTAVPGQAAPGMFGFGGSVGHLDAIDCAP
                     DGGVIVSSALPDDEVYRVERTTFDFSGAELIQQKRKVARVPLEKIDRFPEYGASPFGS
                     CR"
     gene            675..>1948
                     /gene="leuS"
                     /locus_tag="VEV43_12125"
     CDS             675..>1948
                     /gene="leuS"
                     /locus_tag="VEV43_12125"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYP24312.1"
                     /translation="MERDYDFKAIEARWMKVWRETKANAVPEFPSGDSRYALTMFPYP
                     SGDLHMGHVEVFSIHDAITRFWRMTGHEVMTPIGWDAFGLPAENAAIKRGIHPKTWTY
                     DNIAKHRASMERLGFAFDWDRIFYTCEPSYYRWNQWFFLRFYEKGLAYRRAAPANWCP
                     NDKTVLANEQVVAGRCERCGAEVEKRWLTQWFFKITDYADRLVADLELNTGWSEHLKT
                     LQRNWIGRSEGAEVTFRIEEIEEDVVVFTTRPDTLWGATYFALAPEHPLTEKMAAAGG
                     KADELRDFREGVDRLSDIDRASTEREKTGIFLGVHAVNPVNGERIPVWTADYILMDYG
                     TGAIMAGPGHDDRDFEFATKYGLDVRKVVESFDGDTVLPYVGSGRLVNSGPFDGTDVA
                     DAPRVVTAWLDEQGLGKAAVNFRLRDWLISRQR"
BASE COUNT          320 a          664 c          693 g          271 t
ORIGIN      
        1 acgccgagcc cgacgcgacc gaggccccgg ccgcggcccc cgagcccgcc tgcgacaacc
       61 aggaggccgt gctgggcgat ccgtcgctcc gcgccggcag tcccgaggag gccgacgtcg
      121 acggcgacgg cacccccgag accgtctcgg tctactacga ccccgaaggg caggtcgggt
      181 gcgaggcgtt cgtggccgcc gagggagacg agacgaccgt tgccggcgcg ctcgagacgt
      241 ggcggggcga gttcggcctg ccgatgccga cgctccactc ggtcgccgac gtcgacggcg
      301 agcccggtga cgagatcgtc gtgaacatgg ggggcggcgc gtcgacgcag ttcgtcgggc
      361 tcgtggtcgc cgacggggcc gtgctccgtc aggtgaccac cgcggtgccg ggccaggccg
      421 cgcccggcat gttcggcttc ggcgggagcg tcggccacct cgacgcgatc gactgcgcgc
      481 cggacggcgg cgtcatcgtg tcgagcgcgc ttcccgacga cgaggtctac cgagtcgagc
      541 ggacgacgtt cgacttctcg ggggccgagc tgatccagca gaagcggaag gtcgcacgcg
      601 tcccgctcga gaagatcgac cggttcccgg agtacggcgc gtcaccgttc ggcagttgcc
      661 gctagggtca accaatggag cgcgactacg acttcaaggc catcgaggcc cgctggatga
      721 aggtctggcg ggagacgaag gcgaatgcgg tgcccgagtt cccgtccggc gacagccgct
      781 acgcgctcac gatgttcccg tacccctcgg gcgacctgca catggggcac gtcgaggtct
      841 tctcgatcca cgacgcgatc acccggttct ggcgcatgac gggccacgag gtcatgaccc
      901 ccatcgggtg ggacgcgttc ggcctgcccg cggagaacgc cgccatcaag cgcggcatcc
      961 acccgaagac ctggacctac gacaacatcg cgaagcaccg cgcgtcgatg gagcggctcg
     1021 gcttcgcgtt cgactgggac cgcatcttct acacgtgcga gccctcgtac taccgctgga
     1081 accagtggtt cttcctgcgc ttctacgaga agggcctcgc ctacaggcgc gcggcccccg
     1141 cgaactggtg cccgaacgac aagaccgtgc tcgccaacga gcaggtcgtc gcggggcgct
     1201 gcgagcgctg cggcgccgag gtcgagaagc gctggctcac ccagtggttc ttcaagatca
     1261 ccgactacgc agaccggctc gtcgccgacc tcgagctgaa caccggctgg agcgagcacc
     1321 tgaagacgct gcagcggaac tggatcggcc gctcggaggg ggccgaggtc acgttccgga
     1381 tcgaggagat cgaagaggac gtcgtcgtgt tcacgacgcg gcccgacacg ctgtggggcg
     1441 cgacgtactt cgccctcgcg cctgagcacc cgctgacgga gaagatggcg gccgccgggg
     1501 gcaaggccga cgagctgagg gacttccgcg agggggtcga ccgcttgtcc gacatcgacc
     1561 gcgcctcgac cgagcgcgag aagaccggga tcttcctcgg cgtccacgcg gtcaaccccg
     1621 tcaacggcga gcgcatcccg gtgtggacgg ccgactacat cctcatggac tacgggaccg
     1681 gggcgatcat ggcgggcccc ggtcacgacg accgcgactt cgagttcgcg acgaagtacg
     1741 ggctcgacgt gagaaaggtc gtcgagtcgt tcgacggcga caccgtgctg ccgtacgtcg
     1801 ggtccggccg gctcgttaac agtggcccgt ttgacgggac cgacgtcgcc gacgcgccgc
     1861 gcgtcgtgac cgcatggctc gacgagcagg ggctcggcaa agccgcggtg aacttcaggc
     1921 tgcgcgactg gctgatcagc cggcagcg
//
LOCUS       DASQDO010000441         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_842334, whole genome shotgun sequence.
ACCESSION   DASQDO010000441 DASQDO010000000
VERSION     DASQDO010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842334"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VEV43_12130"
     CDS             complement(<1..1001)
                     /locus_tag="VEV43_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HYP24313.1"
                     /translation="MMNERGWPSRLSVAVVALALVAAACGGDDDGGGGGGSAAAGESE
                     LSGEIAVSGSSTVEPISTLVAQQFSAENPDVAISVDGPGTGDGFELFCKGETDVSDAS
                     RPIEAEEAAACEEAGIEYVELKVAIDGITVLTSPENDAITCLDFHDLYALLGPESEGF
                     ENWSDANELAEEVGAGNAPYPDIPLDVTAPGEESGTYDSFAELVLEDIAYEERKIKED
                     DPVVRPDYQASANDNVILQGIEGSSSSLGWVGFSFFQQAGDTVKGLEIDGGDGCVAPT
                     TETIADGSYPIARELFVYVDSAKAEKNPALAAYVDYYLSEDGLASVAEAGYVDLPEE"
     gene            complement(1148..>2328)
                     /locus_tag="VEV43_12135"
     CDS             complement(1148..>2328)
                     /locus_tag="VEV43_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYP24314.1"
                     /translation="TAPDPTEAAPVRFGLASCSNYEAGFFAGYRHLAEREDLSFVLHM
                     GDYLYEYAPGAYGPGPEIGRVHEPDREIVSLEDYRVRHALYKTDPDLQACHAAHPFVT
                     AWDDHEVTNDTHRDGAENHQPDEEGRFLRRRRNAYRAYFEWMPIRPPRPHADPSRIFR
                     RLRLGGLVDLHMLDTRQYRDRQPQDSSDPAKDDPERTITGDRQMAWLKTGLSASRSQW
                     NLVGTQVQIAPVETGEDMPFNVDSWDGYRADRQELLQHIHDAGVSDVVFLTGDIHSSW
                     ACDVPLDSTTYPVTPSVAVELVGTSITSDNLDEITGSPPRTTSIAAENALRADNPHVK
                     YVELDSHGYSVVDANADRLQMDWYYLSDRADPEATATVGASWQTVAGSRRVSPAAEPI
                     "
BASE COUNT          355 a          764 c          791 g          418 t
ORIGIN      
        1 tcctcttcgg ggaggtcgac gtagccggcc tcggcgaccg acgccagacc atcctccgag
       61 aggtagtagt cgacgtacgc cgctagcgcc gggttcttct cggccttcgc cgagtcgacg
      121 tagacgaaca gctcgcgggc gatcgggtac gacccgtctg cgatcgtctc ggtcgtcggg
      181 gcgacgcaac cgtctccacc gtcgatctcc aggcccttca cggtgtcgcc ggcctgctgg
      241 aagaacgaga agccgaccca gccgagcgac gaggagctgc cttcgatgcc ctgcaggatg
      301 acgttgtcgt tcgccgatgc ctgatagtcc gggcggacga cggggtcgtc ctctttgatc
      361 ttccgctcct cgtaggcgat gtcctcgagg acgagctcgg cgaaggagtc gtacgttccg
      421 gactcctcac cgggagcggt gacgtcgagg gggatgtccg ggtagggggc gttgcctgcg
      481 ccgacctcct cggccagctc gttcgcgtcg gaccagttct cgaagccttc ggactcgggg
      541 cccagcagcg cgtagaggtc gtggaagtcg aggcacgtga tggcgtcgtt ctccggcgac
      601 gtgaggaccg tgatgccgtc gatcgcgacc ttgagctcga cgtactcgat cccggcctcc
      661 tcgcaggctg cggcctcctc ggcctcgatc ggccgggaag cgtccgagac gtcggtctcg
      721 cccttgcaga agagctcgaa gccgtcaccg gtgccggggc cgtcgacgga gatagccacg
      781 tcggggttct cggcgctgaa ctgctgtgcc accagggtcg agatcggctc gaccgtcgac
      841 gatcccgaaa ccgcgatctc gcccgacagc tcgctttctc cggcggccgc gctcccccca
      901 ccaccaccac cgtcgtcgtc gccgccacac gcggcagcaa cgagagccag tgcgacgaca
      961 gcgacggaca gcctcgaggg ccatcctctt tcattcatca tctgttcctc ctgtgacgta
     1021 atgtccggat gtctcgctct tgggctcgtt ctgagccgat gtcgcggtga agctagcaac
     1081 cgcatgtgtc acccccacag cgcgttcatg aactagaggt gaactgaggg cggagaccgt
     1141 ctgtcgtcta gatgggttcg gcggccgggc tcacccgacg cgagcctgcg accgtctgcc
     1201 acgacgcgcc gaccgttgcc gttgcttcgg gatccgctcg gtccgagagg tagtaccagt
     1261 ccatctggag ccggtccgcg ttcgcgtcga cgacggagta tccgtgcgag tcgagctcga
     1321 cgtacttgac gtgcggattg tccgcgcgta gcgcgttctc ggcggcgata gacgtcgtcc
     1381 gcggggggct gcccgtgatc tcgtcgaggt tgtccgacgt tatcgacgtt ccgaccagct
     1441 ccaccgcgac cgagggtgtg acgggatagg tggtcgaatc caacggcacg tcacacgccc
     1501 acgaggagtg gatgtcaccc gtcaggaaga ccacgtcgga gacgcccgca tcgtggatgt
     1561 gctgcaggag ctcctggcgg tcggcgcggt agccgtccca cgagtcgacg ttgaacggca
     1621 tgtcctcgcc cgtctcgacc ggcgcgatct gcacctgcgt cccgacgagg ttccactgcg
     1681 accgcgacgc ggagagaccc gtcttcaacc acgccatctg gcgatcgccc gtgatcgtcc
     1741 tctcgggatc gtctttcgcg ggatccgaag agtcctgcgg ctggcggtcc cggtactgcc
     1801 gggtgtccag catgtggagg tcgaccagcc cccccaggcg aagccggcgg aagatgcgcg
     1861 aggggtcggc gtgcggtcga ggagggcgga tcggcatcca ctcgaagtac gcccggtacg
     1921 cgttgcggcg tctgcggaga aaccgtcctt cctcgtccgg ctggtggttc tccgcgccgt
     1981 cccggtgagt gtcgttcgtc acttcgtggt cgtcccacgc agtcacgaag ggatgagccg
     2041 cgtgacacgc ctggaggtcg gggtccgtct tgtagagggc gtgccgcacg cggtagtcct
     2101 cgagcgagac gatctcgcgg tcgggctcgt ggacgcggcc gatctcaggg cccggtccgt
     2161 acgcaccggg ggcgtattcg tagaggtagt cccccatgtg gagcacgaag gacaggtcct
     2221 cgcgctccgc gaggtggcgg tatcccgcga agaaacctgc ctcgtagttg gagcacgagg
     2281 cgagaccgaa cctgaccggg gccgcctcgg tgggatccgg agccgtct
//
LOCUS       DASQDO010000442         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_536224, whole genome shotgun sequence.
ACCESSION   DASQDO010000442 DASQDO010000000
VERSION     DASQDO010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536224"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VEV43_12140"
     CDS             <1..1132
                     /locus_tag="VEV43_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24315.1"
                     /translation="YYADTRLLLGLLNETETQTFALGLSTGLRALWEAEEDFDFTFAS
                     PSTASLDYDLDWATGAAETFLRYRQRDVDSTTLLELDLDTPTPDDFREIREDATERRY
                     DGGFGLQLATDSPSSYEINFAATRIDYDEEADDLAPRTTLQGDGAWILQLTPAVSGTL
                     GATYSYFDAENPRETEIREGEVNAGVIYDPNELLRLRFGLGYANYTREETVGGVRRDV
                     SDDSGYVLTGGLRYLFEEITLDANARLTNAAPETRLSGTLQATYPLPRGLLTGRLFQR
                     YGGGETGDEIRVVGASIGVDREINTASRLGFDVAVARQEDLDTDDPDVDRFNFTATYS
                     YDLTEAVTASVGYRFRSRDEEPESATSNAVFVEVGRAFESGF"
     gene            1289..>1545
                     /locus_tag="VEV43_12145"
     CDS             1289..>1545
                     /locus_tag="VEV43_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HYP24316.1"
                     /translation="MTMTTPTGEALAVDAPANPRGLFWFALAVVSTLPLFRFGFAGLA
                     QEWSRPEFSHGPVIPVLSFYMFLREMKFVPPPSAPVTDRRPG"
BASE COUNT          232 a          578 c          494 g          241 t
ORIGIN      
        1 ctactacgcc gacactcgcc tgctcctcgg tctcctgaac gagaccgaga cccaaacctt
       61 cgccctcggc ctctccaccg ggctgcgcgc gctgtgggag gcggaggagg atttcgactt
      121 caccttcgcc tcgccctcga cggcgtcgct cgactacgac ctcgactggg cgacgggggc
      181 ggcggagacc tttctgcgct accgccaaag ggacgtcgac agcacgacgc tgctcgagct
      241 cgacctcgac acccccacgc ccgacgactt ccgcgaaatc cgggaggacg cgacggagcg
      301 gcgctacgac ggcggcttcg ggctgcagct cgccacggac tcgccttcgt cctacgagat
      361 caacttcgcc gcgacccgga tcgactacga cgaggaggcc gacgatctcg cgccccgcac
      421 caccctgcag ggcgacgggg cttggatcct ccagctcacc cccgccgtct ccggcacgct
      481 gggcgcgacc tattcctatt tcgacgcgga gaacccgcgc gagaccgaga tccgcgaagg
      541 cgaggtcaac gcgggcgtga tctacgaccc caacgaactc ctgcggctgc gcttcggcct
      601 cggctacgcc aactacaccc gggaggagac cgtgggcggc gtgcgccggg acgtctccga
      661 cgacagcggc tacgtgctga cgggcggcct gcgctacctg ttcgaggaga tcacgctcga
      721 cgccaacgcc cggctgacga acgccgcgcc cgagacccgc ttgtccggca cgctccaggc
      781 gacctatccc ctgccccgcg gcctgctcac gggacgtctg ttccagcgct acgggggcgg
      841 cgagaccggc gacgagatcc gggtggtcgg cgcctcgatc ggcgtcgacc gcgagatcaa
      901 caccgcctcg cggctcggct tcgacgtcgc cgtcgcgcgc caggaggatc tcgacacgga
      961 cgatccggac gtcgaccgct tcaacttcac cgccacctat tcctacgacc tgaccgaagc
     1021 cgtcaccgcc agcgtcggct atcgcttccg ctcgcgcgac gaggagcccg aaagcgccac
     1081 cagcaacgcg gtcttcgtcg aggtcggccg ggctttcgaa agcgggttct agaagcgttc
     1141 ctcccccgga tgggccgctt accttcgcgg cgctttgggc tcgttttttc cggcgcgatc
     1201 gcctatagca cgtccgaaat gcgcgcggtc gcctgttcgc ggcgggaccg ccgctgcgta
     1261 agcgggtgat cgagtgacga ggcgcgacat gacgatgacg acgccgacgg gcgaggcgct
     1321 cgcggtcgac gctccggcga acccgcgcgg cctgttctgg ttcgcgctcg ccgtcgtctc
     1381 caccctgccg ctgttccggt tcggcttcgc gggtctcgcc caggaatggt cgcggcccga
     1441 gttcagccac gggccggtga tcccggtcct cagcttctac atgttcctgc gcgagatgaa
     1501 gttcgtgccg ccgccctccg cgcccgtcac ggaccgccgg ccggg
//
LOCUS       DASQDO010000443         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_904008, whole genome shotgun sequence.
ACCESSION   DASQDO010000443 DASQDO010000000
VERSION     DASQDO010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904008"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..607
                     /locus_tag="VEV43_12150"
     CDS             149..607
                     /locus_tag="VEV43_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24317.1"
                     /translation="MDEPRRSAAQTSKWLIPIALGVLLLAYVLPVEETNGGTTVTYSR
                     LLGFRVEGQDLDPRLVAGNVVLHLTWLIVALLALRRTTAKERGVVFGAAALLGIGEVL
                     YLIARLVYDQLVGVNAYLSGLGGILLIVGAVVGMGYVERPEIHESEGARA"
     gene            618..1397
                     /locus_tag="VEV43_12155"
     CDS             618..1397
                     /locus_tag="VEV43_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYP24318.1"
                     /translation="MEQALLKLSPSSATDFKSCPQLFKFRAVDRVPEPVSGAAARGSL
                     VHAVLERLFAEPPRTRTPQRAIDLLEALWAEVKEDVEFRPDGMADPQEDAWLAESARL
                     LRNYFKLEDPRMLSASRLEWWVEYELTDIHLRGVIDRLEERPDGTWILTDYKTGRVPG
                     ESRELSAFFGLRFYALVCWRAFGVIPREIRLVYLADPAVLTLSPNERMLVAFERQMLA
                     LASAIRRAADQDDWRTRPSPFCMSCSFHDRCPAWAPAEGLG"
     gene            complement(1524..>2063)
                     /locus_tag="VEV43_12160"
     CDS             complement(1524..>2063)
                     /locus_tag="VEV43_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24319.1"
                     /translation="VDKADQDGNNGCGNDDDFEDDNEGWCGRKPKPAKPEVKPAGPCD
                     ADAAMPGTQPCKDDDVKPCDKDATMPGTQPCKDDGSKPCDADATMPGTQPCADDEDEV
                     VPDVEPNVVTHPGQPEVPTVVLGERISRDDDAAAPVAARQPTQPGGSTLPFTGASLLV
                     FVGAGLALMGAGIIAARKR"
BASE COUNT          270 a          703 c          742 g          348 t
ORIGIN      
        1 tctcgcgcaa gatgcacgag cacatcggcg gctcgcgcct cgtcgtgatc cccgaagccg
       61 ggcacctgtc gaacctcgag gcgcccgagg ccttcaacgg cgcgctggcg gagttcctgg
      121 gcagccttta gaggaagggg acttcgggat ggacgaaccg aggcgcagcg cggcacagac
      181 gagcaagtgg ctcatcccga tagcgctcgg ggtgctgctc ctcgcgtacg tgctgccggt
      241 cgaagagacg aacggcggga ccacggtgac gtactcccgg ctgctcgggt tcagggtcga
      301 aggccaggac ctggacccgc gcctggtggc gggcaacgtc gtcctccacc tcacgtggct
      361 gatcgtcgcg cttctggctc tgcggcggac gacggcgaag gaacgcgggg tcgtcttcgg
      421 agccgccgcg ctcctcggca tcggcgaagt cctgtacctc atcgcccggc tcgtatacga
      481 ccagctcgtc ggggtgaacg cgtacctgtc ggggctcgga gggatcctgc tgatcgtcgg
      541 tgccgtggtc gggatgggct acgtcgagag gcccgaaatc cacgagagcg agggggcgcg
      601 ggcctagggt tccagtgatg gagcaggccc tactcaagct ctccccttcc agcgccaccg
      661 acttcaagag ctgtccccag ctgttcaagt tccgcgcggt cgaccgggtg cccgagcccg
      721 tctcgggggc cgcggcgcgg ggctcgctcg tccacgcggt gctcgagcgg ctgttcgccg
      781 agcctccgcg cacgaggacg ccgcaacgtg cgatcgacct gctcgaagcg ctgtgggccg
      841 aggtcaagga agacgtcgag ttccggcccg acggcatggc cgacccgcag gaggacgcgt
      901 ggctcgcgga gagcgcgcgg ctgctgcgca actacttcaa gctcgaagac ccccgcatgc
      961 tgagcgcgag ccggctcgag tggtgggtcg agtacgagct caccgacatc cacctccgcg
     1021 gcgtgatcga ccggctcgag gaacgcccgg acgggacgtg gatcctcacg gactacaaga
     1081 cgggccgggt gccgggcgag tcgcgcgagc tctccgcctt cttcggcttg cggttctacg
     1141 cgctcgtgtg ctggagggcg ttcggcgtca tcccgcggga gatcaggctc gtgtacctgg
     1201 ccgacccggc ggtgctgacg ctgagcccga acgaacggat gctcgtcgcg ttcgagcggc
     1261 agatgctggc gctggcctcg gcgatccgcc gggccgcaga ccaggacgac tggcgcacgc
     1321 gtccctcgcc gttctgcatg agctgctcct tccacgaccg ttgcccggcc tgggctcccg
     1381 cggagggcct gggctagcaa gcgtttttct ctgacgcgca gagggggccg gatgtccggc
     1441 cccctccttc gctcccagcg tgcgccatct cgttactggc tcggcggctc tcccaaaccg
     1501 ccgtcatggc tacgccgttc ggttcacctc ttgcgtgctg cgatgatccc cgcccccatc
     1561 agggcgagcc cggcgccgac gaagacgagg aggctcgcgc ccgtgaacgg cagcgtgctt
     1621 ccacccggct gcgtcggctg acgggcggcc acgggggccg cagcgtcgtc gtcacgggag
     1681 atgcgctcgc cgaggacgac cgtcggaacc tccggctggc ccgggtgcgt cacgacgttc
     1741 ggctcgacgt cggggacgac ctcgtcctcg tcgtcggcgc acggctgggt gccgggcatc
     1801 gtggcgtcgg cgtcgcacgg cttcgagccg tcgtccttgc acggctgggt gccgggcatg
     1861 gtggcgtcct tgtcgcacgg cttgacgtcg tcgtccttgc acggctgggt gccgggcatg
     1921 gcggcgtctg cgtcgcaggg gccggcgggc ttgacctcgg gcttggccgg cttgggcttg
     1981 cggccgcacc agccctcgtt gtcgtcttcg aagtcgtcgt cgttgccgca gccgttgttg
     2041 ccgtcctggt cggccttgtc gac
//
LOCUS       DASQDO010000444         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_230988, whole genome shotgun sequence.
ACCESSION   DASQDO010000444 DASQDO010000000
VERSION     DASQDO010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230988"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1316
                     /locus_tag="VEV43_12165"
     CDS             183..1316
                     /locus_tag="VEV43_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24320.1"
                     /translation="MSSAASGDTGSHDFEQQWPDLAKRLDTMLAGKRVPAHKRDDIVQ
                     ETGLRLFRMWGQVDPERPLWALVVTIALNLLRDEARKHPEREVLGLVPDRAADQDVER
                     AGLARLEWRRVQSAMTQLSPDHRSILMAELGDEYATPSRGPNATKMLRMRARRRLNAL
                     LDVASASGVLIGVKVRRLFDFERHVIAIRGLMSSSENLAAPAAAGFLSAVALFAAAAG
                     PFAGDAGAGVLRERIGGSAANLVGVGAGAYGAAASPASGYDLHSERLAAPSDFASNRN
                     VDAGGVEVTTGTGGAATKNKPFRAGTGDHWIEGGAEVGVAGMGVRVGDRGSQTPACLS
                     PGSEGMLPPGYICSDGENTEDGVHADLKVGSDEHKVEVKVDVE"
     gene            complement(1323..>2772)
                     /locus_tag="VEV43_12170"
     CDS             complement(1323..>2772)
                     /locus_tag="VEV43_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24321.1"
                     /translation="GDDVIRGTPGSDVIVARDGDDKVRGGGGADRICGDAGNDRLLGG
                     AGSDRIEMGRIGWMEVASGGDGADLLVGGSRNGNCDGIWRLSGGRGDDRFRAAPGTVC
                     VTMEGGFGDDDLDTGSGSGTMIGGPDDDTMTGSGFFEGGAGDDHMSGDSHSRVVFDDP
                     GGVSVDLAAGTATGPGEGRDTIAGIATVLGTDGDDVIKGDAGANGLYGGAGEDRILGG
                     DGADSIFGQAGDDEVDGGPGDDRVSAVPGNDVVGGGADDDFLTNEVSPDVKRDPAGSA
                     FDGGAGRDWLHLSLHPAGSKTVSIDLAGVSSMGGVLLAVTGIEDVTVYGNEPVELYGD
                     EGPNRLMASGTETVIEGRGGDDVLDPDGVVNTVDGGAGSDLADFNGPWPVVVDLADGT
                     FSEQHRSGTLAGIENVFGSTGDDVIRGDDGPNLLRPSFGNDTAEGRGGDDVLEGSLGD
                     DHLDGGDGTDTLDGDVGTDECVNGESVRNCEG"
BASE COUNT          377 a         1039 c          940 g          416 t
ORIGIN      
        1 catgatctcc cgcgccgtct tggccatatc ggtacctcct cggataggtg gcttcgccgg
       61 tctcctaccc cggcccctga gggggaaaac gagggccaga atgggcgcaa cccctccccc
      121 ggcaggatga gcttggtatg cgtagaatgc tcacgggcgc accaaatctg ggggattcca
      181 ccatgagctc tgcggcgagc ggcgacacgg gcagtcacga cttcgagcaa cagtggccgg
      241 acctcgctaa gcgtctggac acgatgcttg cgggcaaacg cgtcccggcg cacaagcgcg
      301 acgacatcgt ccaggagacc ggcctgcggc tcttccgcat gtggggccag gtcgaccccg
      361 agcgtcccct gtgggcgctc gtcgtgacga tcgctctcaa cctcctgcgc gacgaggccc
      421 gcaagcaccc cgagcgcgag gtcctcggcc tcgtccccga ccgggcggcg gaccaagacg
      481 tcgaacgcgc cggcctcgcc cgtctcgagt ggcggcgggt ccagagcgcg atgacccagc
      541 tcagccccga ccaccgttcg atcctgatgg ccgagctcgg cgacgagtac gccacgccgt
      601 cccgggggcc gaacgcgacc aagatgctcc ggatgcgggc gcggcggcgc ctcaacgcgc
      661 tcctcgacgt cgcgtcggcg agcggcgtcc tcatcggggt caaggtccgg cgcctgttcg
      721 acttcgagcg ccacgtgatc gccatccgcg ggctgatgtc gtcgagcgag aacctcgcgg
      781 cccccgcggc cgcaggcttt ctgtcggccg tcgcgttgtt cgccgcggcg gcgggaccgt
      841 tcgcaggcga cgcgggggcg ggggtgctcc gtgagcggat cgggggcagc gccgcgaacc
      901 tcgtcggcgt cggtgccggt gcgtacggcg cggccgcctc gcccgcttcg ggatacgacc
      961 tccactccga gcgcttggcg gccccttcgg acttcgcctc gaaccgcaat gtcgacgccg
     1021 gcggcgtcga ggtcactacc ggtaccggcg gcgccgcgac gaagaacaag cccttccgcg
     1081 ccgggaccgg cgaccactgg atcgaaggcg gggccgaggt cggcgtcgca gggatggggg
     1141 tccgggtcgg cgaccgcggg agccagacgc cggcgtgcct gtcgccgggc tccgaaggga
     1201 tgctcccgcc gggctacatc tgcagcgacg gcgagaacac cgaggacggc gtgcacgcgg
     1261 acctgaaggt cggctcggac gagcacaaag tggaggtcaa agtcgacgtc gagtgacgtc
     1321 gtctagccct cgcagttcct gacggactct ccgttcacgc actcgtccgt cccgacgtcc
     1381 ccgtcgagcg tgtccgtgcc gtcaccaccg tccaggtggt cgtccccgag cgagccctcc
     1441 aatacgtcgt cgccgccccg accctcggcc gtgtcgttgc cgaagctcgg ccgcaggagg
     1501 ttcggcccgt cgtcgcctcg gatgacgtcg tccccggtcg agccgaagac gttctcgatc
     1561 cccgcgaggg tccctgacct gtgctgttcg gagaaggtcc cgtcggcgag gtcgacgacc
     1621 accggccagg ggccgttgaa gtcggccagg tcactcccgg ccccgccgtc gaccgtgttc
     1681 acgaccccgt ccggatccag cacgtcgtcg ccgccccggc cctcgatcac ggtctcggtc
     1741 ccgctggcca tcaagcggtt cgggccctcg tctccgtaga gctcgacggg ctcgttgccg
     1801 tacaccgtga cgtcctcgat ccccgtgacg gcgaggagga cgccgcccat cgagctgacg
     1861 ccagcgaggt cgatgctgac cgtcttcgag cccgcggggt gcagcgacag gtgcagccaa
     1921 tcgcgccccg cgccgccgtc gaaggcgctg cccgcgggat cgcgtttcac gtccggcgag
     1981 acctcgttcg tcaagaagtc gtcgtccgcg cctccaccga ccacgtcgtt gccgggaacg
     2041 gccgagaccc ggtcgtcgcc gggcccaccg tcgacctcgt cgtcgccggc ctgcccgaag
     2101 atgctgtcgg cgccgtcgcc gccgagtatc cggtcctcgc cggcgccgcc gtagagcccg
     2161 ttcgctcctg cgtcaccctt gatgacgtcg tcgccgtcgg tcccgagcac ggtggcgatc
     2221 ccggcaatcg tgtcgcggcc ctcgcccggc ccggtggcgg tgccggcggc gaggtcgacg
     2281 gagacaccgc cggggtcgtc gaagaccacg cggctgtgcg agtcaccgga catgtggtcg
     2341 tcgccggccc cgccctcgaa gaaccccgac ccggtcatcg tgtcgtcgtc gggaccgccg
     2401 atcatcgtcc cgctgcccga gccggtgtcg aggtcgtcgt caccgaagcc gccctccatc
     2461 gtgacgcaga cggtcccggg cgccgcccga aaccggtcgt cgccacggcc gccactgagg
     2521 cgccagattc cgtcgcagtt cccgttcctg ctgccgccga cgagcaggtc cgctccgtcg
     2581 cctccggacg cgacctccat ccagccgatt cgtcccatct cgatgcgatc ggatcctgca
     2641 cctccgagga ggcggtcgtt gccggcgtcg ccgcagatgc ggtccgcgcc gccgccgccc
     2701 cggaccttgt cgtcaccgtc gcgcgcgacg atcacgtcgg agcccggggt gccccggatg
     2761 acgtcgtctc cg
//
LOCUS       DASQDO010000445         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_292743, whole genome shotgun sequence.
ACCESSION   DASQDO010000445 DASQDO010000000
VERSION     DASQDO010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292743"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..238)
                     /locus_tag="VEV43_12175"
     CDS             complement(20..238)
                     /locus_tag="VEV43_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24322.1"
                     /translation="MLDFATAMSRSPADVPDELRAELWAELGDEGLVQLAAIVAVENF
                     LGRFNRGVGVEAQGYSEGAACLLPDRGD"
     gene            complement(347..607)
                     /locus_tag="VEV43_12180"
     CDS             complement(347..607)
                     /locus_tag="VEV43_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24323.1"
                     /translation="MTTSVSPRARLSWVVQLQQAFTRRALSKLLRRPVGAPESPRVMA
                     HLPLLAPAYGLLEAALLRGRALDPRLGELARARAATIHGCPW"
     gene            656..1510
                     /locus_tag="VEV43_12185"
     CDS             656..1510
                     /locus_tag="VEV43_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HYP24324.1"
                     /translation="MDAAVFEEHRPLLFSLAYRMLGDAGEAEDVVQDVYLRAAGAGDV
                     RDPKAFLVTAVTRKAIDRLRSARRRRETYVGPWLPEPLVGTVADAAEGVERAESLSLA
                     FLVVLESLTPAERAAFLLHDVFGFSYPEVAEALARTEAATRQLAHRARRALESRRRRA
                     QVSPDEHRALLERFVAACAGHDVDGLAALLAREAVVYTDGGGEVQAALRPIEGRERTA
                     RFLVGVTKKAPAGTSAELVPVNGRTGVLVRAGDLTLAVIDVAVADGLVEEVFVVAAPS
                     KLRAAAPA"
     gene            complement(1507..2421)
                     /locus_tag="VEV43_12190"
     CDS             complement(1507..2421)
                     /locus_tag="VEV43_12190"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYP24325.1"
                     /translation="MTPTNEQSTTPTGPVSDLGDVDEIREALDTSEKVDASRIEVSVD
                     GTSVVLRGSVSDSSQGHLAELLVGRYTSDVRNELRVDAGFREGAEEPQAVEEAIPAES
                     EILVGSTDMLAGPDAAVTSDVSRAFEESEPWDPPDEPSLASTPAELRGTSGFGDGGAL
                     DEVSDEPSADQLPAAADLTQQDLEASAAGAAPPSIDETAVPPRDVAEPDPVGLDPLGH
                     TPPEGADDFPPRVPGTEAGIGGVGEGTAGGGSISGVPATETGSLGADTAAADPVRSTG
                     GTMSDAGTDRGPQSREDPPLREDFPDRG"
     gene            complement(2530..>3072)
                     /locus_tag="VEV43_12195"
     CDS             complement(2530..>3072)
                     /locus_tag="VEV43_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24326.1"
                     /translation="PMMLLDTKNPAKPKLLAVAQPMQEFIHSNAWPNAGKDRWALTSG
                     ETWTPGPGTSRCNDKSAGLATWDTSKVKSRKTFMMVDVWRPTNGTVTDGNPVVNAPFG
                     CSSHWFQEHPDFRNGGLVAAGFYNHGTRFLNIAKNGKIEQAGWFLPHGGGTSAAYWID
                     DEVVYAIDYQRGFDVLRYNG"
BASE COUNT          382 a         1074 c         1172 g          444 t
ORIGIN      
        1 agaaggacga ggacgccggc tagtcgccgc ggtccggcag caggcaggcg gccccttccg
       61 agtagccctg ggcctcgacg ccgaccccgc ggttgaagcg gccgaggaag ttctcgaccg
      121 cgacgatcgc ggcgagctgc accagacctt cgtcgcccag ctcggcccac agctccgcgc
      181 ggagctcgtc gggcacgtcc gcggggctgc gcgacatcgc cgtcgcgaag tcgagcacct
      241 tgcgctcacg ctcgtcgaag aggtcggagt cgcggtagcg aggtagcgcc tgcagcttct
      301 cctccgggat cccgaacgcc ctgccgaccg cagagccgag gtccagtcac caggggcatc
      361 cgtggatggt ggcggcgcgg gcccgggcga gctcgccgag ccgcgggtcg agcgcccggc
      421 cccgcagcaa cgcggcttcg agcaacccgt acgcgggagc gaggagcggc aggtgcgcca
      481 tcacgcgggg gctctccgga gccccgacgg ggcggcgcag cagcttcgac agcgcccggc
      541 gggtgaaggc ctgctggagc tgcacgaccc acgagagccg ggcccggggc gaaacgctcg
      601 tcgtcatgcc ctcgtagacg agacatgccc gcaggttgtg acaagctgcg gcgcgatgga
      661 cgcggccgtc ttcgaggaac accgcccgct gctcttctcg ctcgcgtacc ggatgctcgg
      721 cgacgccggc gaggccgaag acgtcgtgca ggacgtgtac ctgcgcgccg ccggtgccgg
      781 cgacgtccgc gacccgaagg ccttcttggt gaccgcggtg acgaggaagg cgatcgaccg
      841 cctccgctcc gcgcgccgga ggcgcgagac ctacgtcggc ccgtggctgc ccgagccgct
      901 cgtcgggacg gtcgccgacg cggccgaagg cgtcgagcgc gcggagtcgc tctcgctcgc
      961 gttcctggtc gtgctggagt cgctgacgcc cgcggagcgc gccgccttcc tgctccacga
     1021 cgtgttcggc ttctcctatc ccgaggtcgc cgaggcgctc gcgaggaccg aggcggcgac
     1081 gcggcagctc gcgcaccggg cccgtcgcgc cctcgagtca aggcgccggc gcgcccaggt
     1141 ctctccagac gagcaccgcg cgctcctcga gcggttcgtg gccgcgtgcg cgggacacga
     1201 cgtcgacggc ctcgccgcgc tgctcgcgcg cgaagccgtg gtctacacgg acggtggcgg
     1261 cgaggtgcag gccgcgctcc gcccgatcga aggccgcgag cgcacggccc ggttcctcgt
     1321 aggcgtgacg aagaaggcgc cggccgggac gagcgcggag ctcgtgccgg tcaacggccg
     1381 cacaggggtg ctcgtgcgcg cgggcgacct gacgcttgcg gtgatcgacg tcgcggtcgc
     1441 cgacgggctc gtggaggagg tcttcgtggt cgcggcgccg tcgaagctgc gcgccgcggc
     1501 ccccgcctaa ccgcggtccg ggaagtcctc tctcagcggc gggtcctcgc gggactgggg
     1561 gccgcggtcg gtgcccgcgt cgctcatcgt cccgcccgtc gaacggacgg ggtcggcggc
     1621 cgcggtgtcg gcgccgagcg acccggtctc cgtcgccggg actcccgaga tcgacccgcc
     1681 tcccgcggtc ccttcgccta cgccgccgat tcctgcctcg gtgccgggga cgcgcggcgg
     1741 aaagtcgtcg gcaccctcgg gcggcgtgtg gccgaggggg tcgagcccga cggggtccgg
     1801 ctccgcgacg tcacgagggg gcacggcggt ctcgtcgatc gaagggggtg cggctccggc
     1861 ggcggatgcc tcgaggtcct gctgcgtcag gtcggccgcg gccgggagct ggtcggcgga
     1921 gggctcgtcc gagacctcgt cgagcgcgcc gccgtcgccg aagccggacg tgccgcggag
     1981 ctccgccggg gtcgacgcca gcgagggctc gtccggcggg tcccacggct cgctctcttc
     2041 gaaggcgcgg ctgacgtcgg aggtgacggc ggcgtcgggg ccggcgagca tgtccgtcga
     2101 gccgacgagg atctcgctct cggccgggat cgcctcctcg acggcctggg gctcctcggc
     2161 cccctcgcgg aacccggcgt ccacccggag ctcgttgcgg acgtcggacg tgtagcggcc
     2221 gacgaggagc tcggcgaggt gtccctggga cgagtccgac acgctgccgc gcaacacgac
     2281 ggaggtcccg tcgacggaga cctcgatgcg gctcgcgtcg accttctcgc tcgtgtcgag
     2341 agcctcgcgg atctcgtcga cgtctccgag gtccgagacg gggcccgtcg gggtcgtgct
     2401 ctgctcgttg gtgggcgtca tcggtgacgt ttcctttcgt gcgcggcggt cgcaggcgcg
     2461 cttacccgcg gccccggaac gcgaaacagg cccccgtcgc cgggggcctg tcggttgagc
     2521 gtgtgtggtc tagccgttgt agcgcaggac gtcgaagccc cgctggtagt cgatggcgta
     2581 gacgacctcg tcgtcgatcc agtaggcagc cgaggtgccc ccgccgtgcg ggaggaacca
     2641 gccggcttgc tcgatcttgc cgttcttggc gatgttcagg aagcgcgtgc cgtggttgta
     2701 gaagccggcg gccacgagac ccccgttgcg gaagtcgggg tgctcctgga accagtgcga
     2761 cgagcagccg aacggagcgt tgacgacggg gttgccgtcg gtgacggtcc cgttcgtcgg
     2821 gcgccagacg tcgaccatca tgaacgtctt gcgggacttc accttcgagg tgtcccacgt
     2881 ggcgaggccg gccgacttgt cgttgcagcg ggaggtgccg gggccgggcg tccaggtctc
     2941 gccggacgtg agggcccagc ggtccttgcc ggcgttcggc caggcgttcg agtggatgaa
     3001 ctcctgcatc ggctgcgcga ccgcgaggag cttcggcttc gccgggttct tcgtgtcgag
     3061 cagcatcatc gg
//
LOCUS       DASQDO010000446         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_782106, whole genome shotgun sequence.
ACCESSION   DASQDO010000446 DASQDO010000000
VERSION     DASQDO010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782106"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VEV43_12200"
     CDS             complement(<1..715)
                     /locus_tag="VEV43_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24327.1"
                     /translation="MGRGLRSELLKLRTTRSTWGVLAGAVAIAVVAFVAPGENAIAEL
                     SEPFHEQQSTFFIGFLLRVILLVLGVRAVTDEWRYGTLTPSLLALPRRGAFLVLKTAA
                     TAGAGLAITALATGAMVLTAAAYAQVNDVTLGEVSFHSLAGMALSGGLWAAIGVGLGA
                     MLKSQLLATVVGIVWLMGVDDMLRERLGDLAVYLPGQAGLALVTAPGAAGLTRAIVTL
                     LAYALVMLVAGVMLTNRRDV"
     gene            complement(717..1622)
                     /locus_tag="VEV43_12205"
     CDS             complement(717..1622)
                     /locus_tag="VEV43_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP24328.1"
                     /translation="MNAGAPAIAVRNLTKAFGATTAVDDLSFDIAPGVVTGFLGPNGA
                     GKSTTLRAILGLVAPTSGEATVLGRRYAELEEPARSVGALLETQQFHPQRTGRNHLAV
                     LAAASGFGHDRVEEVLHEVELTHAAGRKVGGYSLGMRQRLGLAAAMLGDPGVLILDEP
                     ANGLDPAGMRWLRGLLRSYARRGRAVFVSSHLLDEVAHIADEVVVVNKGRLVVHASIE
                     ELRARASRGMRITTPEPERMRHAFERAGIEALQPSESDVLVRAGAETAWEVAAGAGLM
                     VTGLREESLNLEEVFFELTETVREA"
     gene            complement(1619..1870)
                     /locus_tag="VEV43_12210"
     CDS             complement(1619..1870)
                     /locus_tag="VEV43_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24329.1"
                     /translation="MRKPFAIAAGTVAAALVLATTSCVSTGVDSYADFRGAVDSGATC
                     EQLFDMRSNFDDRPSVQDEVDSDLAEIGCEDADSERTDE"
     gene            2057..>2443
                     /locus_tag="VEV43_12215"
     CDS             2057..>2443
                     /locus_tag="VEV43_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24330.1"
                     /translation="MTERRPGARSLACDAGVALLATAIAVSVFFTLPGIGGPAGGVVL
                     GLVVVHNGALALRRLFPAVVVGLVVASALLVLALGWPSVVLGVAAVVVAYSAGAELED
                     AASRLALAALVVSVAIGAAIAEDAGDL"
BASE COUNT          341 a          900 c          829 g          373 t
ORIGIN      
        1 agacgtctcg gcggttcgtg agcatcaccc cggcgacgag catcacgagc gcgtacgcga
       61 gcagcgtcac gatcgcccgc gtcaagcccg cggcccccgg tgcggtcacg agtgctagtc
      121 ccgcctggcc cggcaggtag accgcgaggt cgccgagcct ctcgcgcagc atgtcgtcga
      181 cgcccatcag ccacacgatc cccacgaccg tcgcgagcag ctggctcttg agcatggctc
      241 cgaggcccac gccgatcgca gcccacagcc ctcccgacag cgccatcccc gcgagcgaat
      301 gaaacgagac ctcccccagc gtgacgtcgt tgacctgtgc gtaggcggcc gcggtcagga
      361 ccatcgcgcc cgtcgcgagg gccgtgatcg ccagccctgc acccgccgtc gccgccgtct
      421 tcaggacgag gaacgcccct cggcgcggca gcgccaggag gctcggcgta agcgttccgt
      481 acctccactc gtcggtgacc gccctgacgc ccagcacgag caggatcacg cgcaagagga
      541 agccgatgaa gaacgtcgac tgctgctcgt ggaacggctc ggacagctcc gcgatcgcgt
      601 tctcgcccgg ggccacgaac gcgacgaccg cgatcgcgac cgcaccggcc aagacgcccc
      661 acgtcgatcg cgtcgtccgc agcttcaaca gctccgacct cagtccacgt cccatgtcat
      721 gcctcccgca cggtctcggt gagttcgaag aagacctcct cgaggttcag cgactcctcg
      781 cgcaggcccg tgaccatcaa gcccgcgccg gcggccacct cccacgcggt ctcggccccc
      841 gctcgcacca gcacgtcgct ctcggacggt tggagcgctt cgatcccggc tcgttcgaac
      901 gcgtgcctca tccgttccgg ctcgggagtc gtgatgcgca tgccgcggct tgcgcgcgct
      961 cgaagctcct cgatcgaggc gtgcacgacg agccgtccct tgttgacgac gaccacctcg
     1021 tcggcgatgt gcgcgacctc gtcgaggaga tggctcgaca cgaagaccgc gcggccgcga
     1081 cgggcgtacg aacggagcag ccctcgcagc caccgcatcc cggcggggtc gaggccgttc
     1141 gccggctcgt cgaggatcag gacgccggga tcgccgagca tcgcggccgc gaggccgagc
     1201 cgctggcgca tgccgagcga ataaccgccg accttgcggc cggccgcgtg cgtgagctcg
     1261 acctcgtgca gcacctcctc cactctgtcg tggccgaagc ccgaggcggc cgcgaggacc
     1321 gcgaggtggt tccggccggt gcgctgcgga tggaactgtt gcgtctccag cagcgcgcct
     1381 acggagcgcg cgggctcttc gagctccgcg tagcgcctgc ctagaaccgt cgcctctccg
     1441 gaggtcggcg cgacgaggcc gaggatcgcg cgcagggtgg tgctcttgcc cgccccgttg
     1501 gggccgagga atcccgtgac gacacccggg gcaatgtcga agctcaggtc gtccaccgcc
     1561 gtggtcgcgc cgaaggcctt cgtgaggttc cgcacggcga tcgcgggcgc tccggcgttc
     1621 attcgtcggt cctctccgag tcggcgtcct cgcagccgat ctccgcgagg tccgagtcga
     1681 cctcgtcctg cacgctcgga cggtcgtcga agttcgaacg catgtcgaac agctgctcgc
     1741 acgtggctcc cgagtcgacc gcaccgcgga agtccgcgta gctgtcgacg cccgtgctca
     1801 cgcaggacgt agtcgcgagc acgagagccg cggctaccgt gcccgccgcg attgcgaagg
     1861 gctttctcat ccctgcctcc tttcgttcca tgccgatcac gctagggagg cgggggccgg
     1921 aagtcgtcgg cctggggagt ggacctcgcg gtcgtctcgc gggacacgag gcggctcgcc
     1981 ctacgcccct ggtcggagcg cggcgtacat ctccaggccg atgccccgcg gagggcggat
     2041 gtatagcgtt cgtggcatga ccgagagaag gccgggagcg cgctcgctgg cgtgcgacgc
     2101 gggcgtcgcg ctgctcgcga cggcgatcgc cgtgagcgtc ttcttcacgt tgccgggcat
     2161 cggcggcccg gcaggaggtg tcgtgctcgg cctcgtcgtc gtccacaacg gggcgctcgc
     2221 actgcgccgc ctcttcccgg cggtcgtcgt gggactcgtc gtcgcttcgg ccctgctcgt
     2281 gctcgcgctc ggatggcctt ccgtcgtgct cggggtcgcc gccgtcgtgg tcgcctacag
     2341 cgcgggggcc gagctcgaag acgctgcctc ccgactcgcg ctggcggccc tcgtcgtatc
     2401 ggtggcgatc ggggccgcga ttgccgagga cgccggcgac ctg
//
LOCUS       DASQDO010000447         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_720597, whole genome shotgun sequence.
ACCESSION   DASQDO010000447 DASQDO010000000
VERSION     DASQDO010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720597"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1122
                     /locus_tag="VEV43_12220"
     CDS             25..1122
                     /locus_tag="VEV43_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24331.1"
                     /translation="MGLRGGALVLGGVLAVTSVLTAAAQEGPSCRGQAATHVGTRGPD
                     VITGTRGDDVVVARGGNDKIETLGGDDTICAGRGRDLVRAGKGFQDTVYAGDGDDVVH
                     GGPGRNLLVAGPGDDRMYGSSHYDGFESGRRGERDDEDLFVGRGRSDSFDGDPGDDVI
                     RGGDGTDRIWYFESPAGVTVDLSKGTATGDGRDTLDSFEDVVGTRQDDSVRATNGKNS
                     VFTSTGDDVVYGLGGDDDITDAGGRDELFGGPGRDLVASSGCVSSSDGPTRCQTDAPT
                     PDVLKGGAGADTVGSGPGEDRVFGGGGNDLMSGGDGSDELFGGDGDDELHGGTAAGSE
                     PDADDGDVDELDGGPGTDQCNGEGDTRVACE"
     gene            1206..1379
                     /locus_tag="VEV43_12225"
     CDS             1206..1379
                     /locus_tag="VEV43_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24332.1"
                     /translation="MKRRLGMILVSMVAVGSVQLAVAPAASACADPHCPWSPVTYPVR
                     LLCRDLSPELCPL"
     gene            1396..>1675
                     /locus_tag="VEV43_12230"
     CDS             1396..>1675
                     /locus_tag="VEV43_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24333.1"
                     /translation="MTVARRVLVARCAVVVLLSGCSGGAERDVVAESATPAPSPSPSP
                     SDPCAEYPPQFEATYLPPGFGKRLREGAGLFEGPGTAGYPRDGLVGYYA"
BASE COUNT          241 a          518 c          674 g          242 t
ORIGIN      
        1 gggtgccgag ggtacggtcg ccgcatgggt cttcgtgggg gagctctcgt tctggggggc
       61 gtgctggccg taacgtccgt cctcactgct gccgcccagg aaggcccgtc ttgtcggggg
      121 caagcagcca cgcacgtcgg gacccggggg ccggacgtga tcacggggac gcggggcgac
      181 gacgtcgtcg tggcccgcgg gggcaacgac aagatcgaga cgctcggagg cgacgacacg
      241 atctgcgcgg ggcgcggccg tgacctcgtg cgcgccggca agggcttcca agacaccgtg
      301 tacgccggtg atggcgacga cgtcgtccac ggagggcccg ggcggaacct cctcgtcgcc
      361 gggcctgggg acgatcgcat gtacgggagc tcgcactacg acggcttcga gtctggacgg
      421 cggggcgagc gcgacgacga ggatctcttc gtgggccggg ggcggagcga ctcgttcgac
      481 ggcgacccgg gggacgacgt gatccgtggc ggcgacggca ccgaccgcat ctggtacttc
      541 gagtcgcctg cgggcgtgac ggtggacctc tcgaagggga ccgcaaccgg ggacgggcgc
      601 gacacgctcg actcgttcga ggacgtcgtc gggacgcgac aagacgactc ggtccgtgcg
      661 acgaacggaa agaactcggt cttcacgagc acgggtgacg acgtcgtgta cggcctcggc
      721 ggagacgacg acatcacgga cgccggtggg cgcgacgagc tgttcggggg gccggggcgc
      781 gacctcgtcg cgtcgagcgg ctgcgtctcg tcgagtgacg gacctacccg gtgtcagacc
      841 gacgcgccca cgcccgacgt gctgaaagga ggggcgggcg cggacacagt cggatcgggg
      901 ccgggtgagg accgcgtctt cggaggcggc gggaacgatc tgatgtcggg cggcgacggg
      961 agcgacgagc tcttcggcgg ggacggtgac gacgagctgc acggaggaac ggcggcgggt
     1021 tcggagcccg atgcggacga cggggacgtg gacgagctcg acggggggcc gggcaccgac
     1081 cagtgcaacg gcgagggcga cacccgcgtc gcctgcgagt gaactcactc tttaggagga
     1141 gggagtcgcg ttcgtgcgac tagaaggctg gggggtccgc gtctctccta tgaaggagcc
     1201 gtccgatgaa gcgtcgtctc ggaatgatct tggtcagcat ggtggccgtg ggatccgtcc
     1261 agctcgccgt cgcccccgcg gcttcggcct gtgccgaccc gcactgcccg tggtcgccgg
     1321 tgacgtaccc ggtgaggctg ctgtgcaggg acctgtcgcc ggagctctgc ccgctctaga
     1381 gatagggctg atggtgtgac ggtcgcgcgc cgggtcctag tagcccggtg cgcggtcgtc
     1441 gtccttctgt ccggctgtag cgggggggcg gagcgcgacg tggtggccga gtcggcgact
     1501 ccggcgccct cgccttcgcc ctcgccctcg gacccatgcg ccgagtaccc gccccagttc
     1561 gaggcgacgt acctaccgcc cgggttcggg aagagattgc gggagggcgc gggcttgttc
     1621 gaggggcccg ggacggcggg ctacccgcgg gacggtctgg tcggctacta cgcgg
//
LOCUS       DASQDO010000448         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1088163, whole genome shotgun sequence.
ACCESSION   DASQDO010000448 DASQDO010000000
VERSION     DASQDO010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088163"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VEV43_12235"
     CDS             <1..427
                     /locus_tag="VEV43_12235"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HYP24334.1"
                     /translation="MQESQSLTILYDESCALCRRCRDWLLTQPCLVPVRLLPAGSPEA
                     QSRYASMGPWLGKELVVVDDRGNAWVGPAAFVLCLWATAKYRSASYVLARPSLAPLAE
                     RFFVFVSKRRDRFARWVDDPEPETECSWCDNVMVGRDPR"
     gene            424..1062
                     /locus_tag="VEV43_12240"
     CDS             424..1062
                     /locus_tag="VEV43_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYP24335.1"
                     /translation="MRKDSAAPARPASDTRQKIMDAAVATLREEGIVGTTARAIAQRG
                     DFNQALIFYHFRSIPQLLMEAFERTSDEQIARYEAAAADVSSLHDLVSIARKLHDEDL
                     DSGAVTAVTQLMAAATDPELGGQILDRIDRWITIVERALEEANPARGVLDDAISPRPA
                     AYAISAMFLGIELLTRLDPERAEADAVFDMMADVARVIEQLAPLLGGMSPES"
     gene            complement(1059..2642)
                     /locus_tag="VEV43_12245"
     CDS             complement(1059..2642)
                     /locus_tag="VEV43_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP24336.1"
                     /translation="MRRLALAALLMSLLAAALPAAAADAHTAEDAVVVNPDDGTEIAI
                     TVFRPAGGTAAAPVPVVLHSHGWGGSRETSVDGAAAPYLDAGFGVVSIDQRGHGDSGD
                     QAHVQDPTRETEDVKAVVDYVAALDWVRLEAPNDPVMGAIGGSYGGGYQTMTALDEIA
                     DEGRTRFDALAPQITWYDLPESLAPQKVVRTAWVAALYAAGAQMVPEYVHEAFVWGSA
                     TGQWPDGTLYDQPAPGVPDIDSEFAKHGPSAFVERGIRLDVPVLVRQGASDNLFNFNQ
                     GLKIFERALTDEARAQSYFVSFNGGHALPNALPPGEPADVQAGGGVDACTDFTKTTIE
                     FFRRVFAGTSTGGVLPARYNLTAVDSRTCLRMPSLAGSTTVPVDPVGAGAIVTTAGAG
                     APLQLQVAEGPLAIGGVPELSGKVTSAALDGRAFFGLAIGTTPAGARVIHNNLLPLRR
                     AVPVTDSEFRIELPGVVAEVPAGKNLYLTVTPVSDMFFGHGSRTPGAMVLTDVELDLP
                     VRDCVPAKWKGFGSGRACR"
     gene            complement(2874..3512)
                     /locus_tag="VEV43_12250"
     CDS             complement(2874..3512)
                     /locus_tag="VEV43_12250"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HYP24337.1"
                     /translation="MRPAPVDGSVSWPSDLTVTLSGDERVFSGNNLPGHPTGRFPVEA
                     SDDAYQYDRNPNSITGQDVAFTLDAAPVVLDTPECAGGSVGYMLTGGYLFNGFDARGK
                     DAVAWEVQDACDGHPQATGVYHYHSVSSCAEGSSEGGHSELLGYAFDGFGIYGHYGVD
                     GDALTNEDLDACHGHTHSIEWDGEPRSMYHYHATYEFPYTVSCFRGDAIQVR"
     gene            complement(3526..3963)
                     /locus_tag="VEV43_12255"
     CDS             complement(3526..3963)
                     /locus_tag="VEV43_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24338.1"
                     /translation="MALLRFDHVLVTVDDLEAVTAFFVALGFEKEGTAAVEGDWVDTI
                     VGLPDVRSEIVMLRPPGGGTGLELSKFHRPVPDRGPRAAPVNELGLRNVAFEVEDIHA
                     VVDRLKAEGFELVGGIGNYEDVFLLCYVRGPEGIIVALNEHIG"
     gene            complement(3983..4056)
                     /locus_tag="VEV43_12260"
     tRNA            complement(3983..4056)
                     /locus_tag="VEV43_12260"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4020..4022),aa:Ile,seq:cat)
     gene            complement(4096..>4919)
                     /locus_tag="VEV43_12265"
     CDS             complement(4096..>4919)
                     /locus_tag="VEV43_12265"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYP24339.1"
                     /translation="VRALPGVTQVRAGLDDPARLEEAFAGCEAVAQCAGINREIGSAT
                     YRSVHVDGTRAVVAAAERAGVRRLAIVSFFRARPGTGSPYHDTPWEAEEIVRASPLDW
                     TVLKPGMMYGRGDHMLDHLSKALMTFPVYVGIGNRKVRPLAIDDAVRVLGAALVDGRL
                     PRKTVALLGPTELSFDEAARLVAKAIGKRRLFLRAPIAFHYLLARVSEALMSVPLIAR
                     AQVRMLEEEFVEATRAPDHLPDDLMPKVGFDEASIRAGLPPRLERFGRADLLRRR"
BASE COUNT          696 a         1754 c         1733 g          736 t
ORIGIN      
        1 catgcaggag tcgcagagct tgacgatcct ctacgacgag tcgtgtgcgc tctgccgccg
       61 gtgccgggac tggctgctca cgcagccgtg cctggtgccg gtgcggctcc tccccgccgg
      121 gtccccggag gcgcagtccc gctacgcgtc gatggggccg tggctcggca aggagctcgt
      181 ggtcgtggac gaccgtggca acgcatgggt cggcccggcg gccttcgtcc tgtgcctgtg
      241 ggcgacggcc aagtaccggt cggcctcgta cgtgctggcg cggccctcgc tcgcgccgct
      301 ggccgagcgg ttcttcgtgt tcgtgtcgaa gcgccgggat cggttcgccc ggtgggtcga
      361 cgatcccgaa ccggagaccg agtgctcgtg gtgcgacaac gttatggtcg gtcgcgaccc
      421 cagatgagga aggactccgc tgcgcccgcc cgccccgcgt ccgacacgcg ccagaagatc
      481 atggacgcgg ccgtcgcgac gttgcgcgag gagggcatcg tcgggacgac ggcgcgggcg
      541 atcgcgcagc ggggtgactt caaccaggcg ctgatcttct accacttcag atctatcccc
      601 cagctgctca tggaggcgtt cgagcgcacc tccgacgagc agatcgcgcg ctacgaagcc
      661 gcggcggcgg acgtctcgtc gctgcacgat ctcgtgtcca tcgcgcgcaa gctgcacgac
      721 gaggacctcg actcgggggc cgtgaccgcg gtcacgcagc tgatggccgc ggcgacggac
      781 cccgagctgg gcgggcagat cctcgaccgc atcgaccgct ggataacgat cgtcgagcgc
      841 gcgctcgagg aggcgaaccc ggcccgcggg gtgctcgacg acgcgatctc gccgcgccct
      901 gcggcctacg cgatatcggc gatgttcctg gggatcgagc tgctgacgcg actggacccg
      961 gaacgggccg aagccgacgc ggtgttcgac atgatggccg acgtggcgcg cgtgatcgag
     1021 cagctggcgc cgctgctcgg gggcatgagc cccgagagct agcggcaggc cctccccgag
     1081 ccgaagccct tccacttcgc cggcacgcag tccctcaccg gcaggtccag ctccacgtcc
     1141 gtcagcacca tcgcgcccgg cgtgcgggag ccgtgcccga agaacatgtc cgacaccggc
     1201 gtcaccgtca ggtacaggtt cttgcccgcc ggcacctcgg ccacgacgcc cgggagctcg
     1261 atccggaact cggagtcggt caccggcact gcgcgccgca gcggcagcag gttgttgtgg
     1321 atcacgcgtg cgcccgcggg cgtcgtgccg atcgcgaggc cgaagaacgc gcggccgtcg
     1381 agcgccgccg acgtcacctt gcccgagagc tcggggacgc cgccgatcgc gagcgggccc
     1441 tcggcgacct ggagctgcag cggagctccg gcccccgccg tcgtcacgat cgcacccgcg
     1501 cccacggggt cgacgggaac cgtcgtcgag cccgcgaggc tcggcatacg caggcacgtc
     1561 cggctgtcca ctgcggtgag gttgtagcga gcgggcagca cgccgccggt cgacgtcccg
     1621 gcgaacaccc gccggaagaa ctcgatggtc gtcttcgtga agtccgtgca cgcgtcgact
     1681 ccgccgccgg cctggacgtc cgccggctcc cccggcggaa gcgcgttcgg aagcgcgtgc
     1741 ccgccgttga acgacacgaa gtagctctgc gccctcgcct cgtccgtcag cgcgcgctcg
     1801 aagatcttca gtccctggtt gaagttgaag aggttgtcgc tcgcgccctg ccgcacgagc
     1861 acggggacgt cgagccggat gccgcgctcg acgaacgcgc tcggcccgtg cttggcgaac
     1921 tccgagtcga tgtcggggac gccgggggcc ggctggtcgt agagggtccc gtcgggccac
     1981 tgccccgtcg cgcttcccca cacgaacgcc tcgtggacgt attccggcac catctgcgcg
     2041 cccgccgcgt agagggccgc gacccatgcg gtccgcacga ccttctgcgg agcgaggctc
     2101 tcgggcaggt cgtaccaggt gatctgcggc gccagcgcgt cgaagcgcgt ccggccctcg
     2161 tccgcgatct cgtcgagcgc ggtcatcgtc tggtagccgc cgccgtagct cccgccgatc
     2221 gcacccatga cgggatcgtt cggtgcctcg agccgcaccc agtccagcgc cgcgacgtag
     2281 tcgacgacgg ccttgacgtc ctcggtctcg cgcgtcgggt cctggacgtg ggcctggtcg
     2341 ccgctgtcgc cgtggccgcg ctggtcgatg ctgacgacgc cgaagccggc gtcgaggtag
     2401 ggcgcggcag cgccgtcgac cgaggtctcg cggctcccac cccagccgtg ggagtggagc
     2461 acgaccggca cgggggccgc ggccgtcccg cccgcgggcc ggaagaccgt gatcgcgatc
     2521 tcggtcccgt cgtcggggtt cacgacgacg gcgtcctcgg cggtgtgggc gtcggctgcg
     2581 gccgcgggga gcgcggcggc gagcagggac atgagcaggg cggcaagggc aagccttctc
     2641 acagcgggtc ctccgtgggt ggtcgggcag gcgggaagca ctcgacgctt acccacttcg
     2701 ccggactgct gcgccctcct cccctcgggt cctggactga cggccccggg tggccgaagc
     2761 tcgaaccggc accagacggc ggcctgggga ggtcgaggtc gactggctag ggtcctggcg
     2821 ggaacggctg ggcctggtgg cggcgggagc gatcctgggg gcctagcagt aggtcagcgg
     2881 acctggatcg cgtcgccgcg aaagcagctc accgtgtagg ggaactcgta ggtcgcgtgg
     2941 tagtggtaca tcgaccgcgg ctcgccgtcc cactcgatcg agtgcgtgtg gccgtggcac
     3001 gcgtcgaggt cctcgttcgt cagcgcgtcg ccgtcgactc cgtagtgccc gtagatcccg
     3061 aacccgtcga acgcgtagcc gagcagctcg gagtggcccc cttccgacga gccctcggcg
     3121 cagctcgaga ccgagtggta gtggtacacg ccggtggcct gcgggtgccc gtcgcacgcg
     3181 tcctgtacct cccacgcgac cgcgtccttc ccgcgcgcgt cgaacccgtt gaacaggtag
     3241 ccaccggtga gcatgtagcc gacggagcct ccggcacact ccggcgtgtc gagcacgacg
     3301 ggggccgcgt cgagcgtgaa cgcgacgtcc tggcccgtga tcgagttcgg gttgcggtcg
     3361 tactggtacg cgtcgtccga cgcctcgacc gggaacctcc ccgtcggatg gccgggcagg
     3421 ttgttcccgc tgaagacccg ctcgtcgccg gagagcgtga cggtcaggtc gctgggccag
     3481 ctcaccgagc cgtcgaccgg cgccggccgc aatggggttc gccccttatc cgatgtgctc
     3541 gttcagcgcg acgatgatcc cttccggccc ccggacgtag cacagcagga agacgtcttc
     3601 gtagttgcct atcccgccga cgagctcgaa gccctccgcc ttcagacggt cgacgacggc
     3661 gtggatgtct tcgacctcga aggcgacgtt gcgcagtccc agctcgttca ccggcgccgc
     3721 tcgcggtcct cggtcgggga cgggcctgtg gaacttcgag agctcgagtc ccgtaccgcc
     3781 gccgggcggc cgcaacatga cgatctcgga ccggacgtcg ggcaggccga cgatcgtgtc
     3841 gacccagtcg ccctcgacgg cggcggtgcc ctccttctcg aacccgagcg cgacgaagaa
     3901 cgccgtaacg gcttcgagat cgtcgacggt gacgaggacg tggtcgaaac gcagcagggc
     3961 catcgtcggt cctttcgtgt agtgggggcg gcagggctcg aacctgcgac caacggatta
     4021 tgagtccgcc gctctgacca actgagctac gcccccggcg tcccaacgat agtgccgccc
     4081 gcgcagcgca tcgacctagc gcctgcgcag gaggtccgct ctgccgaagc gctcgagccg
     4141 cggcgggagc ccggccctga tcgaggcctc gtcgaacccg accttcggca taaggtcgtc
     4201 cggcagatgg tcgggcgccc gcgtcgcctc tacgaactcc tcttcgagca tccgcacctg
     4261 cgcccgcgcg atcagcggga cgctcatcaa cgcctcggac acgcgggcga ggaggtagtg
     4321 gaacgcgatg ggggcccgca ggaacaggcg ccgcttcccg atcgccttcg cgacgaggcg
     4381 tgcggcctcg tcgaacgaga gctccgtcgg cccgagcagc gcgaccgtct tacgcggcag
     4441 ccggccgtcg acgagcgcgg cccccagcac tctcaccgcg tcgtctatcg ccagtggccg
     4501 gaccttgcgg ttgccgatgc cgacgtagac ggggaacgtc atcagtgcct tgctcagatg
     4561 gtcgagcatg tggtcgccgc gcccgtacat catcccgggc ttcagcaccg tccagtcgag
     4621 cggcgaagcg cgcacgatct cctcggcctc ccacggcgtg tcgtggtacg gcgagccggt
     4681 accgggccgc gcccggaaga agctgacgat cgcaagcctc cgcacgccgg cgcgctctgc
     4741 cgcagccacg acagcgcggg tcccgtcgac gtgcacggac cggtaggtcg cgctgccgat
     4801 ctcccggttg atgcctgcgc attgcgcgac ggcctcgcat cctgcgaacg cttcttcgag
     4861 ccgtgcgggg tcgtcgagac ccgcccgcac ctgcgtcacg cccggcagcg cccggacct
//
LOCUS       DASQDO010000449         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_292948, whole genome shotgun sequence.
ACCESSION   DASQDO010000449 DASQDO010000000
VERSION     DASQDO010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292948"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VEV43_12270"
     CDS             complement(<1..224)
                     /locus_tag="VEV43_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24340.1"
                     /translation="MKLYAETSWQRSRQIAADAAVALWIVVWVRIGTWIHELVNRLAE
                     PGRSVESAGGRISSAMEAAGDRLGGLPGVG"
     gene            272..>1592
                     /gene="glmM"
                     /locus_tag="VEV43_12275"
     CDS             272..>1592
                     /gene="glmM"
                     /locus_tag="VEV43_12275"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009199195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYP24341.1"
                     /translation="MARLFGTDGVRGVANRDLTPDLALALGRAAGLVLAPGGGRVVVG
                     RDTRLSGPMLHAALTAGLCSAGASVLGAGILPTPGVAWLTRREDAAAGAVISASHNPV
                     PDNGIKFFSAVGTKVSADEETSIEELLAQTPDELPTGEAIGGVDPVEDGASRYVEHLL
                     TSIDGSLGGLRVVVDCAYGAAFAVGPEAFRRAGAEVVALHDSPDGARINVDCGSTSLD
                     TLAKVVVSEGAAFGVGFDGDADRALAVDETGAVIDGDRIIALAALHLKERGELDGGLV
                     VTTVMANLGLRRALEAAGIEVVTVPVGDRFVAEAMTEHGAVIGGEQSGHVIFAEHATT
                     GDGVLTGLKLAEIVAASGRPLSDLAAVFEPYPQVLLNVRIADRGALEGAAEVWERVRA
                     AEESLGESGRVLVRASGTEPLVRVMVEAEDPATADRVARDLAALVESK"
BASE COUNT          198 a          573 c          613 g          208 t
ORIGIN      
        1 tccccgacgc cgggcaggcc gccgagtcga tcgcccgccg cttccatcgc ggacgagatc
       61 cgcccgccgg cgctctcgac tgacctcccg ggctcggcga gccggttgac gagctcgtgg
      121 atccacgtgc cgatgcggac ccacacgacg atccagagcg cgacggcggc gtcggccgcg
      181 atctgccgcg agcgctgcca cgacgtctcc gcgtagagct tcaccgcacc cctctacccg
      241 gcccggcgga aaaccacgcg aagatcgccc gatggccagg ctcttcggga ccgacggcgt
      301 gcgcggagtc gcgaaccgcg acctcacacc ggacctcgct ctcgcgctcg gacgcgcggc
      361 ggggctcgtg ctcgcgcccg gcgggggccg cgtcgtcgtg ggccgggaca cgcggctatc
      421 cggcccgatg ctgcacgccg cgctgaccgc gggcctgtgc tcggcgggtg cgtccgtgct
      481 cggcgccggg atcctcccca cgccgggggt cgcgtggctc acgcgccggg aggacgccgc
      541 ggcgggggcc gtgatctccg cgtcgcacaa ccccgtcccc gacaacggga tcaagttctt
      601 ctccgccgtc gggacgaagg tctcggcgga cgaggagacg tcgatcgagg agctgctcgc
      661 gcagaccccg gacgagctcc ccacgggcga ggcgatcggt ggcgtcgatc ccgtggagga
      721 cggagcgagc cgctacgtcg agcacctcct cacgtcgatc gacggctcgc tcggcggcct
      781 gcgggtcgtc gtcgactgcg cgtacggcgc ggctttcgcc gtcgggcccg aggcgttccg
      841 gcgcgcgggg gccgaggtcg tcgcgctcca cgactcgccg gacggagccc gcatcaacgt
      901 cgactgcggc tcgacgtcgc tggacacgct cgcgaaggtc gtcgtgtccg agggcgcggc
      961 gttcggcgtc ggcttcgacg gggacgcgga ccgagcgctc gccgtggacg agaccggcgc
     1021 cgtgatcgac ggcgaccgga tcatcgccct cgcggcgctg cacctgaagg agcgcggcga
     1081 gctcgacggc gggctcgtag tgacgacggt gatggcgaac ctcggcctgc gccgtgcgct
     1141 cgaggcggcc gggatcgagg tcgtgacggt cccggtcggc gaccgcttcg tggccgaggc
     1201 gatgaccgag cacggcgcgg tcatcggggg cgagcagtcg ggtcacgtca tcttcgccga
     1261 gcacgcgacg accggcgacg gtgtcctcac cgggctgaag ctcgccgaga tcgtcgcggc
     1321 gtcggggcgg ccgctctccg acctggccgc cgtcttcgag ccctacccgc aggtcctgct
     1381 caacgtccgc atcgccgacc ggggggccct cgagggggcc gccgaggtct gggagcgggt
     1441 ccgggcggcc gaggagtccc tgggcgagtc cggccgcgtg ctggtccggg cctcggggac
     1501 cgagccgctg gtgagggtga tggtcgaggc cgaggacccg gctacggcgg acagggtggc
     1561 gagggacctg gcggcgctgg tcgagtcgaa gc
//
LOCUS       DASQDO010000450         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_415530, whole genome shotgun sequence.
ACCESSION   DASQDO010000450 DASQDO010000000
VERSION     DASQDO010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415530"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..685
                     /locus_tag="VEV43_12280"
     CDS             218..685
                     /locus_tag="VEV43_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24342.1"
                     /translation="MWRSPALVLSIVALLAAPALAEPEPIDWDWKERHRGGRDEQVWG
                     GLARNRDELRRLWKLYDQPGKPPVIGFEKNVAVVAGAIGSSSCPARLYDVRLNRERKR
                     VVVRMYEEDSGTDVCTADVVPRTFSVSIARRDLKPLSAGELKLRPREIEDPGE"
     gene            682..1149
                     /locus_tag="VEV43_12285"
     CDS             682..1149
                     /locus_tag="VEV43_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24343.1"
                     /translation="MKAAVAGAVAAVLLASSALAEPETLAFVRKGAQERGPDETAWGD
                     VGHDRDRLDVLWDRYRQKGKPPAIAFDEKVAVVAGTGGSGSCPTRLHDLRLHRERGRV
                     VVRVYTEAPPGDGGCTDDWVPKTFTVSVKRSDLKPLKPRAIDVRVRRIEDPDG"
     gene            complement(1155..2075)
                     /locus_tag="VEV43_12290"
     CDS             complement(1155..2075)
                     /locus_tag="VEV43_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24344.1"
                     /translation="MRKLQAALLALMLAAAACGGSTPDEGAGDDIAALDGSGDEKAPG
                     GGKDGGDDKPKGAGTGTKKPGGKKPGGSKAAPGATPQPAGGGGSDSNAGGGSGNAGGS
                     PQPAAAPAPVPQGEYRYDTEGSRTISGNTSDLPKVTTLAAGAPSDGVQRQTRDLRDSE
                     GNGTLTDTDLVYGPGGVSLSYVKVTSRFQGGITDVREFKLARPQLVAPAGGGPGFNRS
                     FTMQGSGTKAKVTITAHRWENVPVSGEQVRALMVETEIRFSGALEGYQRTIAWFWPKH
                     LLTLREQVQTDVRNGPIRLQSTYRAQIQQI"
     gene            complement(2072..>3018)
                     /locus_tag="VEV43_12295"
     CDS             complement(2072..>3018)
                     /locus_tag="VEV43_12295"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYP24345.1"
                     /translation="ASLVLAPGVNPEADREQVRRFAQRLSSVEGIARVDAATGSYVEG
                     ELLFFSEKVGARFAADGATYFSAVLAPTVEAQSLEAEDVVHSIRALDAPFAFEVAGPS
                     ADLVDGKEGAFARIPIAVLWIALVTFVTLFLLFGSVLVPLKAVVLNLLSLTATFGAMV
                     WVFQDGNLSGVLDFTATGTIVLSNIILMFCIAFGLSMDYEVFLLSRVKEEYDATGDND
                     LAVARGLDRTGRVVSAAAALIAIVFVAMISSGISFIKMFGLGLALAVVMDATLVRGAL
                     VPAFMKLMGPANWWAPAPLRRLHDRFGIREATAEGTRT"
BASE COUNT          447 a         1073 c         1057 g          441 t
ORIGIN      
        1 ggtttcagcg gcggggattg gatcgagctc ggatgggtcg tccgctccgc gtgatcgccc
       61 tgtggtcggt cacgaagcgc gccatctagc gtccttccgg actcttgcta gtcgccgatc
      121 agaggtgggg tacccgaccg cgatcaggcc gggacgaacg gggacggggg ccgcgacggc
      181 cctgtcgttt ccccctgcgg cgctctacga tcgcgggatg tggcgttccc cggccctcgt
      241 gctgtcgatc gtggccctgc tcgcggcccc ggccctcgcg gagcccgagc cgatcgactg
      301 ggactggaag gagcgtcacc gcggcgggcg cgacgagcag gtctggggcg gcctcgcccg
      361 caaccgcgac gagctgcgcc ggctgtggaa gctctacgac cagccgggaa agcccccggt
      421 catcggcttc gagaagaacg tagcggtcgt cgcaggggcc atcgggtcga gctcgtgtcc
      481 ggcccgtttg tacgacgtgc gcctcaaccg cgagcggaag cgcgtcgtcg tgcggatgta
      541 cgaagaggac tccgggacgg acgtctgcac cgccgacgtc gtcccgagga cgttttcggt
      601 gtcgatcgcg cggcgcgacc tgaagccgct ctccgcgggc gagctgaagc tgcggccgcg
      661 cgagatcgag gatccgggcg agtgaaggcc gcagtcgcag gtgccgtggc ggccgtgctg
      721 ctcgcgagct ccgcgctggc ggagccggag acgctcgcct tcgtccgcaa gggggcccag
      781 gaacgggggc cggacgagac ggcgtgggga gacgtgggcc acgaccgcga ccgcttggac
      841 gtgttgtggg accgctaccg gcagaagggc aagcccccgg cgatcgcgtt cgacgagaag
      901 gtggcggtgg tagcggggac cggagggtcg ggctcgtgcc cgacgcgcct tcacgacctc
      961 cgcctgcacc gcgagcgagg gcgcgtcgtc gtgcgcgtct acaccgaagc cccgcccggc
     1021 gacggcggct gcacggacga ctgggtgccg aagacgttca cggtgtcggt gaagcgctcg
     1081 gacctgaagc cgctgaagcc gcgcgcgatc gacgtgcgag tcagacggat agaagacccg
     1141 gacggctagc gaccctagat ctgctggatc tgggcccggt aggtgctctg cagccggatc
     1201 ggcccgttgc gcacgtccgt ctgcacctgc tccctcagcg tcagcaggtg cttcggccag
     1261 aaccacgcga tcgtgcgctg gtagccctcg agcgctcccg agaacctgat ctcggtctcg
     1321 accatcagag cgcgcacctg ctcgccggaa accggcacgt tctcccaacg gtgcgccgtg
     1381 atcgtcacct tcgccttcgt gcccgagccc tgcatcgtga acgaccggtt gaaccccggc
     1441 ccccctcccg cgggcgcgac gagctgcggg cgcgcgagct tgaactcgcg cacgtcggtg
     1501 atcccgccct ggaagcgcga cgtcaccttc acgtaggaga gcgagacgcc gccggggccg
     1561 tacacgaggt cggtgtcggt cagcgtcccg ttgccctcgg agtcacgcag gtcgcgcgtc
     1621 tgccgctgga ctccgtccga cggagcgccg gcggcgagcg tcgtcacctt cggcaggtcc
     1681 gacgtgttgc cggagatcgt gcgggagcct tcggtgtcgt agcggtactc gccctgcgga
     1741 accggagcgg gcgcggccgc gggctgcggc gacccgccgg cgttacccga cccaccaccg
     1801 gcgttcgagt ccgagccccc gccacccgca ggctgcggcg tggcgccggg cgcggccttc
     1861 gaaccgccgg gcttcttgcc gccgggcttc ttcgtcccgg tcccggcccc cttgggcttg
     1921 tcgtccccgc cgtccttgcc tccccccggc gccttctcgt cgccgcttcc gtcgagcgcg
     1981 gcgatgtcgt cacccgcccc ctcgtcgggc gtgctcccgc cgcacgcggc cgcggcgagc
     2041 atcaaggcca gcagtgccgc ctgaagcttc ctcatgtcct agtcccttcc gccgtcgctt
     2101 cccggatgcc gaatcgatcg tggagtctgc gcagcggcgc aggcgcccac cagttcgcag
     2161 gtcccatgag cttcatgaac gccggcacga gcgcgccgcg caccagcgtc gcgtccatca
     2221 cgaccgcgag cgcgagcccc agcccgaaca tcttgatgaa cgagatcccc gagctgatca
     2281 tcgcgacgaa cacgatggcg atcagcgcgg cggcggcgga aactacgcgt cccgtgcggt
     2341 cgagcccccg cgcgaccgcg aggtcgttgt ccccggtcgc gtcgtactcc tctttcacgc
     2401 gcgacagaag gaagacctcg tagtccatcg agagcccgaa cgcgatgcag aacatcagga
     2461 tgatgttcga cagcacgatc gtccccgtcg cggtgaagtc cagcacgccc gacaggttgc
     2521 cgtcctggaa cacccacacc atcgccccga acgtggcggt gaggctcagc aggttgagca
     2581 cgaccgcctt gaggggcacg agcacgctcc cgaacagcag gaagagagtg acgaacgtca
     2641 cgagcgcgat ccacagcacc gcgatcggga tgcgcgcgaa cgcgccctcc ttgccgtcga
     2701 cgagatcggc cgagggcccg gccacctcga acgcgaacgg cgcgtcgagg gcccggatgg
     2761 agtgcacgac gtcttcggct tcgagcgact gcgcctcgac cgtgggtgcg aggaccgcgg
     2821 agaagtacgt cgcgccgtcg gccgcgaagc gcgcgccgac cttctcggag aagaagagca
     2881 gctcgccctc gacgtagctg ccggtcgccg cgtcgacccg tgcgatcccc tctaccgagg
     2941 acagcctctg cgcgaagcgc cgcacctgct cgcggtcggc ctcggggttg actccggggg
     3001 cgagcacgag cgacgcag
//
LOCUS       DASQDO010000451         5575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_904519, whole genome shotgun sequence.
ACCESSION   DASQDO010000451 DASQDO010000000
VERSION     DASQDO010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904519"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VEV43_12300"
     CDS             complement(<1..407)
                     /locus_tag="VEV43_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYP24346.1"
                     /translation="MQIGMVGLGRMGANMSRRLLDGGHEVVACDVDPAAVDQLVAEGA
                     IGAESLETLVEKLTPPKAVWAMLPAGEVTGSTIARLAELLDAGDVVIDGGNSRHVDSV
                     ARASLLAEAGIEFVDCGTSGGIWGLTEGYCLMVG"
     gene            461..601
                     /locus_tag="VEV43_12305"
     CDS             461..601
                     /locus_tag="VEV43_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24347.1"
                     /translation="MNEDPKPQENREGDEPPSEGSGTTDDEGGAERAGLGRSEAEDKI
                     SK"
     gene            666..1127
                     /locus_tag="VEV43_12310"
     CDS             666..1127
                     /locus_tag="VEV43_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24348.1"
                     /translation="MRTKALVSTAVLLVLSTAVPGEAARPRRDMARYHYSDNNHHFGG
                     PQVGFGTWDPADSYAFDLRKGERSVSVMVLDDRERPVAATVVQIQWDGDYGSARVGHA
                     VTHEEFCGRTAAPVPVVPDLPVEIFLQKGVCDDGTPSLPTEGDIVVDFHRG"
     gene            1220..1381
                     /locus_tag="VEV43_12315"
     CDS             1220..1381
                     /locus_tag="VEV43_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24349.1"
                     /translation="MRKAARVALVAGFLMSLLAGPAQAGPCGALGDVCQEILYRPMDQ
                     LCRVYDICL"
     gene            1493..3334
                     /locus_tag="VEV43_12320"
     CDS             1493..3334
                     /locus_tag="VEV43_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24350.1"
                     /translation="MSRRRFSAVLVPVAIAAAVVPLDAAAAAASCKGLRATITGTGRD
                     EQLKGTRGADVIYAGAGNDVVLGRGGRDVICGGSGNDVLSGGSDADRLFGDDDADVLD
                     GGPGSDFVNGGKGHDVASFESARRGVRVDLGGRVARGQGDDNLDGIESAAGGRGDDVI
                     RGTRRINLLVGGLGDDRLIGLGGNDVIQPLEDDDFADGGAGQDVLSYSLSTSAVAIDP
                     VDGDGTERLRQFEALQGSPYPDVIGGTDGDDVLLGWLGGDEFRPRGGDDYVDGEGFGP
                     DRDQVVYQDSPRRVVIHLVATPGGDDHVGEANGDGTDVLVTIDGATGSRFDDEMVGRG
                     WTDSFGGLGGDDRISIGTGGTASGGEGADVMTGTGFLFGNGGDDRMTGGTGSLGGAFS
                     GGDGDDVMTGGEGADIFFPGPGADSVSGGAPSEVVDGNLLRQDTVSFVTAFGGVVVDL
                     TAGTASGEGADALDTIEDVDGSPYDDVITGNELQNVLSGGSGPDRIDALSGGDAIDGG
                     FGDDEMSGGEGDDLLIGNEGADSADGGPGRDACDAEEVTTCETAPARRAVPEALPGGG
                     TPGVLQASRWARHLRKTIQAITDSGNVTRPTSPISQLRNLSRVISPR"
     gene            3357..4250
                     /locus_tag="VEV43_12325"
     CDS             3357..4250
                     /locus_tag="VEV43_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYP24351.1"
                     /translation="MDGSLTLLVDAPSLFYRALFSTPDSVTTPEGKPINAAHGFFRML
                     ARLVADHDPDFIACAADESWRPRWRVELVDSYKDFRAAPGSAQEAAEQRLAGQVPILY
                     GFLEMCDIAVVGHPEAEAEDVIGTLAQRAPGRVLIFSGDRDLFQLVRDPEVAVLFPIR
                     GVSQLAVVDETYVSERYGIPGRAYRDFAVLRGDPSDGLPGVRGIGEKIAAALVKRYGS
                     IDAILEAADGDDPSVPLAKVRREADYVRRASQVASIVTDLPLEPVDATRPRDLPRDAI
                     VSAAEQVGLRGPIAALLDALS"
     gene            complement(4301..4675)
                     /locus_tag="VEV43_12330"
     CDS             complement(4301..4675)
                     /locus_tag="VEV43_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24352.1"
                     /translation="MRKIVLAAVTAALVGTMVPAAEAGHRPQEACSESGDVCQSTKKV
                     NGVRKLTITLGGKYFSRYKLCVKAPDDSTTCKKFKIKDTGPAYGSSIRWDKHFPDAGA
                     GAYTVTWKNRGTRIGARLGFHE"
     gene            4787..>5575
                     /locus_tag="VEV43_12335"
     CDS             4787..>5575
                     /locus_tag="VEV43_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24353.1"
                     /translation="MSRKLALLGALMLSLVPVPAAAQDQFCAGRRATIVGTDRRDIIK
                     GTPGRDVIVAGAASDIINAAGGADIVCDGPGNDIVRGAGGRDRLLNGEGGDALRGGPG
                     ADRLGHPQSFETFEGSFDHLVGGGGDDTLFSTSEQQRDSYDGGAGNDTLDLRLLSAGA
                     TVDLEVGRIDGAGEGFTSEIENVSGTGYSDVLRGDDGLNVLFGGDVSAGFFGPTYPPG
                     GVSDTLEGRGGDDTIDGGTGDDTISGGDGDDAIRDGRGHDVIDAG"
BASE COUNT          828 a         1902 c         2059 g          786 t
ORIGIN      
        1 ccgaccatca ggcagtagcc ctcggtcagg ccccagatgc cgccggaggt cccgcagtcg
       61 acgaactcga tcccggcttc tgcgagaagc gaggcgcgcg ccaccgagtc gacgtggcgc
      121 gagttcccgc cgtcgatcac gacgtcgccg gcgtcgagca gctccgcgag ccgtgcgatc
      181 gtcgagcccg tgacctcgcc ggccgggagc atcgcccaca cggccttcgg cggcgtgagc
      241 ttctcgacga gggtctcgag ggattcggcg ccgatcgcgc cctcggcgac gagctggtcg
      301 actgcggcgg ggtcgacgtc gcaggccacg acctcgtggc cgccgtcgag gaggcgtcga
      361 gacatgttcg cccccatgcg gccgagcccg accattccta tctgcatccg cgtcaagagt
      421 agcctcggca cttacacgag ccgagccgga gggggctgcg atgaacgagg acccgaagcc
      481 gcaagagaac cgcgagggcg acgagccccc gtccgagggt tccggcacga ccgacgacga
      541 aggcggtgcc gagcgcgccg gcttgggtcg ctccgaggcc gaggacaaga tcagcaagta
      601 gctagcccgg ccctcggtta cccgcgcccc cgccgcccgt acgactgaga tggaaggagg
      661 cggtggtgag gacgaaggcg ctcgtttcga cggcggtgct cctggtgctc tcgacggcgg
      721 ttcccggcga ggccgcccgc ccgcgccgcg acatggcccg ctaccactac agcgacaaca
      781 accaccactt cggcgggcct caggtcggct tcgggacgtg ggatcccgcg gactcgtacg
      841 cgttcgacct tcgcaagggt gagaggtcgg tgtcggtgat ggtcctcgac gatcgcgaac
      901 gcccggtggc ggcgacggtc gtccagatcc agtgggacgg cgactacgga agcgccaggg
      961 tcggccatgc ggtcacccac gaggagttct gcggacgcac cgcggcgccg gtcccggtcg
     1021 tccccgacct ccccgtcgag atcttcctcc agaagggcgt ctgcgacgac ggcacgccgt
     1081 cgctcccgac cgagggcgac atcgtcgtcg acttccatcg cggctagcgc ccccgaaatc
     1141 ccccatccgg gtcagggaga agccgagccc cgaggcgaat cgtgctccgt cgggtcgggc
     1201 ggaacaacga ggaggagaca tgcgcaaggc cgcacgtgtg gcgctggtgg cggggttctt
     1261 gatgtcgttg ctggcggggc cggcgcaagc gggcccctgc ggagccctcg gcgacgtgtg
     1321 ccaggagatc ctgtaccggc cgatggacca gctctgcagg gtctacgaca tctgcctgta
     1381 gcaccttcga agacgacggc cggaggctcg gacctccggc cgtcgccgcg tccggccccc
     1441 gtggcgtgtt cgataatcga gcggtcgtcg cgtggtctcg gagggggcgg gcatgagtcg
     1501 caggcggttc tcggcggtat tggtcccggt ggcgatcgcg gcggcggtcg tcccactcga
     1561 cgcggcggcc gcggccgcgt cgtgcaaggg gctccgcgcg acgatcaccg gcaccggccg
     1621 ggacgagcag ctgaagggca cgcggggagc cgacgtcatc tacgcggggg ccggcaacga
     1681 cgtcgtcctg ggccgcggcg gaagggacgt gatctgcggg ggttcgggga acgacgtgct
     1741 ctccggcggg agcgatgccg accggctctt cggtgacgac gacgccgacg tgctcgacgg
     1801 ggggccgggc tccgacttcg tcaacggcgg gaaggggcac gacgtcgcgt cgttcgagtc
     1861 ggcgaggcgc ggggtccggg tcgacctcgg cggccgtgtc gcgcgcggcc agggcgacga
     1921 caacctcgac ggcatcgagt cggcggcagg gggccggggt gacgacgtca tccgcgggac
     1981 gcgccggatc aacctgctcg taggggggct cggcgacgac cgtctaatcg ggctcggggg
     2041 gaacgacgtc atccagccgc tcgaggacga cgacttcgca gacgggggcg cggggcagga
     2101 cgtcctctcc tattcgctgt cgacgagtgc cgtcgcgatc gacccggtcg acggcgacgg
     2161 caccgaacgt ctgcggcagt tcgaggcgct gcaggggtcg ccctaccccg acgtgatcgg
     2221 cgggacggac ggtgacgacg tcctgctggg ctggctgggc ggcgacgagt tccgcccgcg
     2281 tggcggtgac gactacgtcg acggggaagg gttcgggccg gaccgggacc aggtcgtcta
     2341 ccaggactct ccgcgccgcg tcgtgatcca cctcgtcgcc acgcccggcg gcgatgatca
     2401 cgtcggcgag gcgaacggcg acgggacgga cgtgctggtg acgatcgacg gcgccacggg
     2461 gtcgcgcttc gacgacgaga tggtcggccg cggttggacg gactcgttcg gcgggctcgg
     2521 gggagacgac cggatctcga taggcacggg cggcaccgcg agcggaggcg agggagccga
     2581 cgtcatgacc gggaccgggt tcctcttcgg caacggcggc gacgatcgga tgacgggcgg
     2641 aaccggatcc ctcggtggcg cgttctcggg cggcgacggc gacgacgtca tgacgggcgg
     2701 tgagggggcc gacatattct tcccgggccc cggcgcggac tcggtatccg gaggagcgcc
     2761 ctcggaggtc gtcgacggga acctcctgcg ccaggacaca gtgtctttcg tcacggcatt
     2821 cggcggcgtg gtcgtcgacc tcacggccgg gacggcttcg ggagagggcg ccgacgcgtt
     2881 ggacacgatc gaggacgtgg acggctcgcc gtacgacgac gtgatcacgg gcaacgagct
     2941 gcagaacgtg ctctccggcg gcagcgggcc ggaccggatc gacgcgttgt cgggcggcga
     3001 cgcgatcgac ggagggttcg gcgacgacga gatgtccgga ggggaggggg acgacctgct
     3061 catcggcaac gagggcgccg actcggccga cggggggccg ggccgggacg catgcgacgc
     3121 ggaagaggtg acgacatgcg agaccgcgcc ggcacggcgc gcggtgcccg aggcgctacc
     3181 gggtggcggg acgccgggcg tgcttcaggc gtcgcgctgg gcgcgccact tgcggaagac
     3241 gatccaggcg atcaccgaca gcgggaacgt gacgaggccg acgagcccca tctcccagtt
     3301 gaggaacttg agccgggtca tctcgccacg gtagcgcggg cttaggctgc cgcgccgtgg
     3361 acggctcgct cacgctcctc gtcgacgcac ccagcttgtt ctatcgggcg ctgttctcga
     3421 cccccgacag cgtcacgacc ccggagggca agcccatcaa cgcagcccac gggttcttcc
     3481 ggatgctcgc ccggctcgtg gccgaccacg acccggactt catcgcgtgc gccgccgacg
     3541 agagctggcg tccgcgctgg cgcgtcgagc tcgtcgactc ctacaaggac ttccgcgcgg
     3601 cccccggcag cgcgcaggag gccgcggaac aacggctcgc cggccaggtc ccgatcctct
     3661 acgggttcct cgagatgtgc gacatcgcgg tggtcggcca tcccgaggcc gaggcggagg
     3721 acgtcatcgg gacgctcgcg caaagggcgc cgggacgcgt gctgatcttc tcgggcgacc
     3781 gcgacctgtt ccagctcgtg cgcgaccccg aggtcgccgt gctcttcccg atacgcggcg
     3841 tgagccagct agcggtcgtc gacgagacct acgtgtccga gcgctacggg atcccgggcc
     3901 gcgcctaccg ggacttcgca gtcctgcgcg gcgacccttc cgacggtctg ccgggcgtcc
     3961 gcggcatcgg cgagaagatc gcggccgcgc tcgtcaaacg ttacgggagc atcgacgcga
     4021 tcctcgaagc cgccgacggt gacgacccga gcgtcccgct agcgaaggtg cggcgcgagg
     4081 ccgactacgt gcgccgcgcg tcgcaggtcg cgtcgatcgt caccgacctg ccgttggagc
     4141 ccgtcgacgc gacgcggccg cgggacctcc cgcgcgacgc gatcgtctcc gccgccgagc
     4201 aagtcggcct gcgggggccc atcgccgcgc tcctcgacgc gctgtcctag tctcggcgcg
     4261 tctcggcggc gagatgcgag gagagcgatc cgcatctagg tcactcgtgg aatcccaggc
     4321 gcgcgccgat gcgcgtgcct cggttcttcc acgtcaccgt gtacgcgccg gcccccgcgt
     4381 ccgggaagtg cttgtcccac cggatgctgc tcccgtaagc cggtcccgtg tccttgatct
     4441 tgaacttctt gcacgtcgtc gagtcgtcgg gcgccttcac gcacagcttg tagcgcgaga
     4501 agtacttgcc gccgagcgtg atcgtgagct tccgcacgcc gttcaccttc ttggtcgact
     4561 ggcacacgtc acccgactcg gagcacgcct cctgcggacg gtggcccgcc tccgccgccg
     4621 gcaccatcgt gcctacgaga gctgccgtca ccgctgccaa cacgatcttc cgcacgttcg
     4681 acctcctcta gccgcctagg ggccagcgta gccgcgaccg acgcttctgg cgcttacctc
     4741 ccgacccgtg ccagcatgga ggccggttct aacgaagggg gtcttcttgt cacgcaagct
     4801 cgcgctcctc ggcgcgctga tgctctcgct cgtccccgtt cccgctgccg cccaggacca
     4861 gttctgcgcc ggtcgtcggg cgacgatcgt cggcacggac cgtcgcgaca tcatcaaggg
     4921 aacgcccggt cgcgacgtga tcgttgccgg agcggcgtcc gacatcatca acgcagccgg
     4981 gggcgcggac atcgtctgtg acggcccggg caacgacatc gtgcgcggcg cgggtggccg
     5041 cgaccggctg ctgaacggag aaggaggcga cgcgctgcga ggaggcccgg gcgcggacag
     5101 gctcgggcac ccacagagct tcgagacgtt cgaaggctcg ttcgaccacc tcgtcggggg
     5161 cgggggagac gacacgctgt tctcgacttc ggagcagcag cgggacagct acgacggcgg
     5221 cgcgggcaac gacacgctcg acctgaggct gctgtccgcc ggcgcgacgg tggacctcga
     5281 ggttggcagg atcgacggcg cgggagaggg cttcacctcg gagatcgaga acgtctcggg
     5341 caccggctac tccgacgtgc tccgcggtga cgacgggctc aacgtcctct tcggcggcga
     5401 cgtctcggcc gggttcttcg ggccgacgta tccccccggc ggcgtatccg acacgctcga
     5461 gggccggggt ggcgacgaca ccatcgacgg ggggacgggc gacgacacga tctcgggcgg
     5521 cgacggcgac gacgcgatcc gcgacgggcg cgggcacgac gtgatcgacg caggt
//
LOCUS       DASQDO010000452         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1088564, whole genome shotgun sequence.
ACCESSION   DASQDO010000452 DASQDO010000000
VERSION     DASQDO010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088564"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEV43_12340"
     CDS             complement(<1..194)
                     /locus_tag="VEV43_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYP24354.1"
                     /translation="MRAERGCSWAREELSARLDGESRPADAAALDDHLATCAACRRHE
                     RDLEDVRRALRVQPAEEVPDL"
     gene            complement(184..702)
                     /locus_tag="VEV43_12345"
     CDS             complement(184..702)
                     /locus_tag="VEV43_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24355.1"
                     /translation="MGAARGGDTGAFEDLVRIYQGHVWRLALHLLHDPDLASDATQEA
                     FVRAYRFLPRYRGDSRFSTWLFSIARNCALDEARKASRRKRITDRAEAEPQPTSKDVG
                     VAIEVREALASLPPDLREPVVMIDMFGISYKEVATILKMPEGTVKSRVHRAREMLARS
                     LTPRSGEDAGAR"
     gene            complement(816..1508)
                     /locus_tag="VEV43_12350"
     CDS             complement(816..1508)
                     /locus_tag="VEV43_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HYP24356.1"
                     /translation="MDPEPTAFEEERTSTSGMASALVTLALFASSLGLAAVEAEGAAG
                     APEPTASPAATVDVLAELVVSPELDGDDYDRHAFKHWVDVDKDGCDTRQEVLLLESRA
                     QTTLEPSKCRVAGGEWLSLYDDVVVTNPSSLDIDHMVPLKEAWESAAATWDDGQREAY
                     ANDLDAEMALIAVTASSNRSKSDRDPAQWKPPDTTYWCTYATAWASVKVKWSLTADTA
                     EVAALEEMLAAC"
     gene            complement(1508..2473)
                     /locus_tag="VEV43_12355"
     CDS             complement(1508..2473)
                     /locus_tag="VEV43_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24357.1"
                     /translation="MFVFTEFWSFVGAGVALGGLLLLVHSLISGTQKRGTHGRHRGVK
                     ALVIGADGRASTSKAQVLMWTLAVLYAFTFLLLWGRSIGCDSPTPLCQQAQRARGLFG
                     DVLEEPLETEYYALLGLPTAAAVAAKALTVNKVAKGEVVKEKAAGDQKGIGRGLAEVV
                     SADNGRTDLIDFQYFAFNLLALAYFLFQFVARPADGLPEIPPTLIALTGVAVAAYTGK
                     KALETEAPTISAVIPRRLVVGEATTLTVVGSGFGHAPARLAIDGVTIPVAESEWTDRK
                     IETDVAPDALDRLRVRGRTRAADVTVVTAEAAVSRPHRVELERPD"
     gene            2603..2902
                     /locus_tag="VEV43_12360"
     CDS             2603..2902
                     /locus_tag="VEV43_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24358.1"
                     /translation="MAYLGNLTCRDCGLTFTSRWGSVASADEYRCANDHVVHVEPHSG
                     TVLAVDGGAAARPRTLVDLRGRCPECSTELATGRLPKCPVCGGRDHDVLLSGTIG"
     gene            2945..>3294
                     /locus_tag="VEV43_12365"
     CDS             2945..>3294
                     /locus_tag="VEV43_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HYP24359.1"
                     /translation="MPPETDKRLTDRRALILRAIVSHYVGTGEPVGSKVLVERYRLGV
                     SPATVRNEMSALEDGGYIYQPHTSAGRIPTDAGYRYFVDEMPEGARLPAAEAHRIRQF
                     FVEPRWELEEALRQT"
BASE COUNT          452 a         1194 c         1158 g          490 t
ORIGIN      
        1 aggtccggga cctcttccgc cggctgcacg cgcagcgccc gcctcacgtc ttcgaggtcg
       61 cgctcgtgac ggcggcacgc cgcgcaggtc gcgaggtggt cgtcgagggc ggccgcgtcg
      121 gcggggcgag actcgccgtc gagtcgcgcc gacagctcct cgcgtgccca cgagcacccg
      181 cgctcagcgc gcaccggcgt cctccccgga gcgcggcgtc agcgaccgcg cgagcatctc
      241 gcgggcccgg tgcacccggc tcttcacggt cccctcgggc atcttcagga tcgtcgctac
      301 ctccttgtag gagatgccga acatgtcgat catcacgacg ggctctctga ggtcgggagg
      361 caacgacgcc agcgcctcgc gcacctcgat ggcgacgccg acgtccttcg acgtgggctg
      421 cggctcggcc tcggcccggt cggtgatccg cttccgcctc gacgccttgc gcgcctcgtc
      481 gagcgcgcag ttccgggcga tcgagaacag ccaggtcgag aagcgggagt cgccgcggta
      541 gcgggggagg aaccggtagg cgcgcacgaa cgcctcctgc gtcgcgtcgg aggcgaggtc
      601 ggggtcgtgg agcaggtgga gcgcgagccg ccagacgtgg ccctggtaga tccggacgag
      661 gtcttcgaac gcgccggtat cgccgccccg cgcggccccc acaagaccag gatcaggctc
      721 gtccacggtg ccctccaagg gccctcagcg gtcgttctca tcctgccact ccacggctgt
      781 gagactccgg gagggggccg gaagttcggg cggcctcagc aggccgccag catctcctcc
      841 agggcggcga cctctgcggt gtcggcggtg agcgaccact tgaccttgac gctcgcccag
      901 gcggtcgcat acgtgcacca gtacgtggtg tccggaggct tccactgggc cgggtcgcgg
      961 tccgacttcg agcggttgct ggacgcggtg acggcgatga gggccatctc ggcgtcgagg
     1021 tcgttcgcgt acgcctcgcg ctggccgtcg tcccacgtcg cggccgcgga ctcccacgcc
     1081 tccttgagcg ggaccatgtg atcgatgtcg aggctcgacg ggttcgtcac gacgacgtcg
     1141 tcgtagagcg agagccactc gccccccgcg acgcggcact tcgacggctc gagcgtcgtc
     1201 tgggcgcgcg actcgagcag caggacctcc tggcgcgtgt cgcagccgtc cttgtccacg
     1261 tcgacccagt gcttgaacgc atgccggtcg tagtcgtcgc cgtccagctc gggcgagacg
     1321 acgagctcgg ccagcacgtc cacggtcgcc gccggcgacg cggtcggctc gggggcgcct
     1381 gccgctccct cggcctcgac cgcggcgagg ccgaggctcg aagcgaacag cgcgagcgtg
     1441 acgagcgcgc tcgccatccc cgacgtggag gtgcgctctt cttcgaaggc ggtgggctcg
     1501 gggtccatca gtcgggcctt tcgagctcga cgcgatgggg ccgtgagacc gcggcctcgg
     1561 cggtgaccac cgtgacgtcg gcggcgcgtg tgcgtccgcg gacgcggagg cgatcgagcg
     1621 cgtcgggggc gacgtcggtc tcgatcttgc ggtcggtcca ctcgctctcc gctacgggga
     1681 tggtgacgcc gtcgatcgct agacgcgccg gcgcgtgccc gaagccgctt ccgacgacgg
     1741 tcagcgtcgt ggcctctccg acgacgaggc ggcgcgggat cacggcgctg atcgtggggg
     1801 cctcggtctc cagcgccttc ttgccggtgt aagcggcgac cgccaccccc gtgagagcga
     1861 tcagcgtcgg agggatctcg gggaggccgt ccgcgggccg cgcgacgaac tggaacagga
     1921 agtaggcgag cgcgagcagg ttgaacgcga agtactggaa gtcgatgagg tcggtgcggc
     1981 cgttgtcggc actgacgacc tcggccagcc ccctgccgat tcccttctgg tcaccggccg
     2041 ccttctcctt gaccacttcg cccttggcga ccttgttcac cgtcagcgcc ttcgccgcga
     2101 ccgcggccgc ggtcgggagg ccgagcagcg cgtagtactc ggtctcgagc ggctcctcga
     2161 ggacgtctcc gaagaggccg cgggcccttt gcgcctgctg acagaggggg gtcgggctgt
     2221 cgcacccgat cgagcgcccc cacaagagca ggaacgtgaa cgcgtacagg accgcgagcg
     2281 tccacatcag gacctgggcc ttcgacgtcg acgcccgccc gtccgccccg atcacgagcg
     2341 ccttcacccc ccggtggcgc ccgtgcgtcc cgcgcttctg ggtcccggag atgagggagt
     2401 gcacgagcag gagcagcccg ccgagcgcca cgcccgcccc cacgaagctc cagaactcgg
     2461 tgaacacgaa catccccggc ccccctgctc ggtccggcct taagcgtcgc gcgcaggccc
     2521 tcccgtgaca aggcgattta caaactcaac gagctctttc gactcggccc ccgttgggcg
     2581 cggcggctgc catgcttgtg ccgtggccta cctcgggaac ctgacctgcc gcgactgcgg
     2641 gctgacgttc acgtcgcgct ggggctcggt cgcgtccgcg gacgagtacc gctgcgcgaa
     2701 cgaccacgtc gtccatgtcg agccgcactc ggggacggtc ctggccgtcg acggtggcgc
     2761 cgccgctcgc ccccggaccc tggtcgacct gcggggccgg tgcccggagt gctcgaccga
     2821 gctggcgacg gggcggctgc cgaagtgccc ggtctgcggg ggccgcgacc acgacgtcct
     2881 gctctcgggg acgatcggct agcactcccg taccgcgagt gctaagtcgg ataggatccg
     2941 gaccatgccc cccgagaccg acaagcgcct caccgaccgg cgcgccctca tcctgcgcgc
     3001 gatcgtgtcc cactacgtcg ggaccggcga gcccgtcggc tcgaaggtgc tcgtcgagag
     3061 gtaccggctc ggcgtctcgc cggccaccgt ccgcaacgag atgtccgccc tcgaagacgg
     3121 cggctacatc taccagccgc acacgtcggc cggaaggatc ccgaccgatg ccggctaccg
     3181 gtacttcgtc gacgagatgc ccgagggcgc gcgcctgccg gccgccgagg cccatcgcat
     3241 ccgccagttc ttcgtcgagc cgcgctggga gctcgaagag gccctgcgcc agac
//
LOCUS       DASQDO010000453         7927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1149269, whole genome shotgun sequence.
ACCESSION   DASQDO010000453 DASQDO010000000
VERSION     DASQDO010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149269"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..342)
                     /locus_tag="VEV43_12370"
     CDS             complement(97..342)
                     /locus_tag="VEV43_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24360.1"
                     /translation="MRKITTVAAAAAALMALAVPTASAQTGDPAGCTTVLVDTLTNGA
                     LPELVEVTYTPPGTVTVDADGVFAAAQPFVTWVDCVV"
     gene            complement(368..625)
                     /locus_tag="VEV43_12375"
     CDS             complement(368..625)
                     /locus_tag="VEV43_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24361.1"
                     /translation="MKRSLRAVTAIGAVAAAMALMTPAASADPGDAQDCTTVLIQSLL
                     TTTPEPELVEVTYTPPATVNVSADGAIAQAQPFVTWVDCVV"
     gene            802..2172
                     /locus_tag="VEV43_12380"
     CDS             802..2172
                     /locus_tag="VEV43_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP24362.1"
                     /translation="MATCLVAAAAQAATGVRAEAAAGLGPEGGQRALVVFDHEVGRGT
                     LDRLAAAGVTRARVFDSIDTVATFAPAPSAYATISAWSDVVAVEDDTPLRYANNVAKR
                     DTRVTGVRSGEAPLSAPYTGKGVTVAVVDSGVDTSHPDLRDRVLANVDYEHAPVLDPI
                     TDGAYSEAAAERPVSTDEFGHGTHVAGIVAGTGAVAQGADMSGVAPEAALVNCKMGFG
                     DALESTELACFEWILEHRDDPRFPGGIRVVTSSWGADATASAGLPLRALVREMTKAGI
                     VMVFAAGNDGPAQKGSSTVLRYPNSMEAVITVGATCKSEEYMPFNCSGPLAVAAFSSQ
                     GKEVDVVAPGSDIWSTKSAVSVLGTRNALVQAQGNPVPGATVADQTANRAFYITLGGT
                     SMSTPHVAGIVALMLEANPGLRPAEVESILTETARDLGPRGFDTGYGHGLADALRATR
                     AAESRR"
     gene            complement(2246..3676)
                     /locus_tag="VEV43_12385"
     CDS             complement(2246..3676)
                     /locus_tag="VEV43_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYP24363.1"
                     /translation="MERPVQTGQFRRRLTVAFVLVAGVAAGTVGLGAYLLVHDIRFDD
                     FVERSLRDAEANFLSIDHAPAGDAAIDDTIKRLSRRAPAAIVIEQGDTVFLSGAGVDV
                     RDVPDGLEAPSSHDEGQTLPHEDATIAGEDYLVVAAPFGTDGGSMWFFYSKTAMQNGL
                     RDLAGVTWRLWLVVMVGAALVGRALARRTLRPVSQASSAARSLAEGLLATRLPVETED
                     EFGVWAASFNEMADALETKIAELVQARDRERQFTSDVSHELRTPVTALVSSASLLEER
                     LEALDPDARWMAQQMIAEARRLRGLVDDLLEISRLQFGREVVRTSEVDLGRLVDRVIA
                     NQRWETSVAYEPASVTVTTDKSRVERIVTNLVHNAVVHGRESVRVAVRAVEDSVLVEV
                     RDDGPGIPALHLPHVFDRFYKADPARPGGSGLGLAIAAEHARVLGGSIEVTSVEGSGA
                     AFTLRLPTTGSGPAEATDRTSAEVVA"
     gene            complement(3679..4359)
                     /locus_tag="VEV43_12390"
     CDS             complement(3679..4359)
                     /locus_tag="VEV43_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018340429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP24364.1"
                     /translation="MDERVLLVEDDPAIGEITARGLGQAGFRVTKEEDGRQSLVRFGQ
                     EDFDLVVLDVMLPSLDGLGVCREIRAHSPVPILMLTARADTADVVLGLEAGADDYVTK
                     PFEMPELVARVRALLRRVSVGPEPHVISAGDLRIDVPGHKVSRAGSPVDLTATEFKLL
                     VALARHAGQVMTREMLLELVWDYDYLGDSRVVDMAVKRLREKVEPAAGGNRLIQTVRG
                     VGYRIDHG"
     gene            complement(4374..4679)
                     /locus_tag="VEV43_12395"
     CDS             complement(4374..4679)
                     /locus_tag="VEV43_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24365.1"
                     /translation="MNMIDLVSPGLVDAETLRELGPLLLEQGVDLVSAPSPEALRDGT
                     PLYVDVNADPVHVQRLIALAHVRMLFVLDRGTVVGVVDTADLCARAESLEWPTGASP"
     gene            complement(4810..5436)
                     /locus_tag="VEV43_12400"
     CDS             complement(4810..5436)
                     /locus_tag="VEV43_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24366.1"
                     /translation="MRLTKRSRLGAVGATAVAMLAWGIPVTEAQDRPECGDTNVICGS
                     PGDDRIVGTAAHEYIYTYGGDDFVDGGGGDDHVFLGPGDDVAVGGSGHDRLRGQEGHD
                     TLRGNDGWDDLRGGSGRDVLTGHTGNDALYGWNAADILRGGAGVDNLHGGRHSDLIRG
                     GGDDDILTGKSGNDTLHGNAGDDTLACGKGRDTARGGKGKDDLRSCER"
     gene            5934..6410
                     /locus_tag="VEV43_12405"
     CDS             5934..6410
                     /locus_tag="VEV43_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24367.1"
                     /translation="MNVLRRGVAAAAVVGMLQLVAAGPAAATGTATCVPLPGSTPAVG
                     ADLDGDGTEDVRLPAISDVELCAGANVVLTEPVTIEREQCGGFGSCMRYYVTYEVSGY
                     AQKDLTFCYSADGTRVCTATDPAPIPVDLVSPQTMCFGWDLRGGYPCPSGGPVELG"
     gene            complement(6436..7020)
                     /locus_tag="VEV43_12410"
     CDS             complement(6436..7020)
                     /locus_tag="VEV43_12410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP24368.1"
                     /translation="MTWDPDTYPGTIRAEIEDYDELQERVVDATRGIAAQSILELGVG
                     AGETAARLLTVHEGAHLTGVDSSPEMLAGAARILPADRVTFVEADLGDPLPPGPFDLV
                     VSALAVHHLEGEHKTGLFERIAPALAPGGVFVLGDVVVPDDPADAVIENEPGYDFPSS
                     VPDQLAWLARAGFEAEVVWTRRDLAVIRAAQPSR"
     gene            7059..7568
                     /locus_tag="VEV43_12415"
     CDS             7059..7568
                     /locus_tag="VEV43_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24369.1"
                     /translation="MVSNRRVSLTGLRRVFAGSLVAMALVVVLTWFVSREGSTDAPTM
                     SPRTILILLAAYGALDLVLVRAALRRTPPQTATVEAVGDWFRALTLLGHSLGMSSFLM
                     GFVGVFLANEWTLVLAGVPVALIVFWLVGPTARRVANAQRRLNSAGVAVELTDAVEVS
                     PQTAGGLAR"
     gene            complement(7541..>7927)
                     /locus_tag="VEV43_12420"
     CDS             complement(7541..>7927)
                     /locus_tag="VEV43_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HYP24370.1"
                     /translation="RRTALDATLRWDLLNLGSEAVLRRAGAGRALRLRYEDFVAQPRA
                     TVEQARRLLGETSAGSPFVDDRTVTLGVNHSIAGNPSRFSTGTLVLEDRSDWREGQSR
                     IDRWITTAVAFPFLHRYGYRARPPAV"
BASE COUNT         1121 a         2831 c         2786 g         1189 t
ORIGIN      
        1 gccgacgcgg tgggtaccgc cagggccatg agcgcggccg ctgcggccgc gaccgtcgtg
       61 atcttcctca tctcgtcctc ctcggattcg tgtgggtcag acgacgcagt cgacccaggt
      121 gacgaacggc tgcgccgccg cgaacacgcc gtcggcgtcg acggtcaccg ttcccggcgg
      181 ggtgtaggtg acctcgacca gctcggggag ggcaccgttg gtgagcgtgt cgacgagcac
      241 cgtggtgcag ccggcaggat cgcccgtctg cgccgacgcg gtgggtaccg ccagggccat
      301 gagcgcggcc gctgcggccg cgaccgtcgt gatcttcctc atctcgtcct cctcggattc
      361 gtgtgggtca gacgacgcag tcgacccagg tgacgaacgg ctgcgcctga gcgatcgcgc
      421 cgtcggcgct gacgttgacc gtcgcgggcg gcgtgtaggt gacctccacg agctcgggct
      481 ccggagtggt cgtcaggagc gactggatga gcacggtcgt gcagtcctgc gcatcccccg
      541 gatcagccga cgccgccgga gtcatcagtg ccatggcggc cgccaccgcg ccgatggccg
      601 ttaccgctct gagagatcgc ttcaaggatc cgcctcctcg tcgagttccc caataccgac
      661 cggcatcgct tcgacgccgg ccgctggacc tccttccgtg tcggtggccg ctgtgggcgt
      721 tccggaagcg cccgggcgca gggcggacga ggcccggcgg cgaacctccg gggcatgtcc
      781 cgcaccctca ctcgtgctct ggtggccact tgcctggtcg ccgccgcagc ccaggccgcg
      841 acgggggtcc gggccgaggc cgccgccggc ctcggccccg aaggcgggca gcgcgctctg
      901 gtcgtgttcg accacgaggt cggccggggg accctcgacc gcctcgccgc tgccggcgtc
      961 acccgggccc gggtcttcga ctcgatcgac acggtcgcga cgttcgctcc ggccccgtcg
     1021 gcctacgcca cgatctccgc gtggagcgac gtcgtcgccg tcgaggacga caccccgctc
     1081 cgctacgcga acaacgtcgc gaagcgcgac acgcgagtga cgggggtgcg gtccggcgaa
     1141 gcccccctga gcgcgccgta caccggcaag ggcgtcacgg tcgccgtggt cgactcgggc
     1201 gtggacactt cgcacccgga cctcagggac cgagtgctcg cgaacgtcga ctacgagcac
     1261 gctcccgtgc tcgacccgat caccgacggc gcgtactccg aggccgccgc ggaacgaccg
     1321 gtctcgacgg acgagttcgg acacggcacg cacgtcgccg ggatcgtcgc cggcacgggc
     1381 gccgtagccc aaggtgcgga catgagcggc gtcgctcccg aagccgcctt ggtcaactgc
     1441 aagatggggt tcggcgacgc gctcgagagc accgagctcg catgcttcga gtggatcctc
     1501 gagcaccggg acgacccgcg gttccccggc ggcatccgcg tcgtcaccag ctcgtggggc
     1561 gcagacgcga cggcgtcggc cgggctcccg ctgcgcgcgc tcgtgaggga gatgacgaag
     1621 gcggggatcg tgatggtgtt cgccgcgggc aacgacggcc cggcccagaa gggctcctcc
     1681 accgtcctcc ggtatcccaa cagcatggaa gcggtcatca cggtcggcgc cacgtgcaag
     1741 tccgaggagt acatgccgtt caactgctcc ggccccctcg ccgtcgccgc gttctcgagt
     1801 cagggcaagg aggtcgacgt ggtggcgccc ggctcggaca tctggtcgac gaaatcggcg
     1861 gtgagcgtcc tcggcactcg caacgcgctc gtgcaggcac aggggaaccc ggttcctggc
     1921 gcgaccgtcg ccgaccagac agcgaaccgc gccttctaca tcacgctcgg gggcacgtcg
     1981 atgtcgacac cccacgtcgc ggggatcgtc gcgctgatgc tggaggcgaa cccggggctg
     2041 cgtccggcgg aggtcgagtc gatcctcacc gagacggccc gcgacctggg gccgcgcggc
     2101 ttcgacaccg gttacgggca cgggctcgcc gacgcgctcc gagcgacacg cgcggccgag
     2161 agcagacgct agccgcagct cccgccggca ccggcgagag gatctccccc gggccccccg
     2221 tcgagctcgt cgtccgcggg agcgatcacg cgaccacctc cgccgaagtc cggtccgtag
     2281 cctcggccgg ccccgacccc gtcgtgggaa gtcgcagcgt gaacgcggcc cccgagccct
     2341 cgacgctggt gacctcgatc gagccaccga ggacgcgggc gtgctcggcg gcgatggcga
     2401 gaccgagccc gcttcccccc ggacgagcgg ggtcggcctt gtagaagcgg tcgaagacgt
     2461 gcgggaggtg gagcgccggg atcccgggcc cgtcgtcacg aacctccacg aggaccgagt
     2521 cctccacggc tcggaccgcg acgcgcacgc tctcacgccc gtggacgacc gcgttgtgga
     2581 cgaggttcgt gacgatgcgc tcgactcggg acttgtccgt cgtcacggtc accgacgcgg
     2641 gctcgtacgc gaccgacgtc tcccagcgct ggttcgcgat cacgcggtcg acgagccggc
     2701 cgaggtcgac ctcgctcgtc ctgacgacct cgcgtccgaa ctgcaggcgc gagatctcga
     2761 gcaggtcgtc gacgaggcct cgcagacgcc tcgcctccgc gatcatctgc tgcgccatcc
     2821 agcgcgcgtc gggatcgagc gcctccaggc gctcttcgag cagcgaggca gaagacacga
     2881 gcgcggtcac cggcgtccgg agctcgtgcg agacgtcgga ggtgaactgg cgctcgcggt
     2941 cgcgcgcctg cacgagctcc gcgatcttcg tctcgagcgc gtcggccatc tcgttgaacg
     3001 acgcggccca gactccgaac tcgtcctcgg tctcgacggg caaccgcgtc gccagcaagc
     3061 cctccgccag cgaccgcgcg gcggagctcg cctgggagac cggtcgcagg gtccgccgcg
     3121 ccagcgccct tccgacgagc gcggccccca ccatgacgac gagccacagc ctccaggtga
     3181 cccccgcgag gtcgcgcaga ccgttctgca tcgcggtctt cgaatagaag aaccacatcg
     3241 agccgccgtc ggtgccgaag ggggccgcga ccacgaggta atcctcacct gcgatcgtcg
     3301 cgtcctcgtg cggaagcgtc tgtccctcgt cgtgtgacga cggcgcctcg aggccgtcgg
     3361 gtacgtccct cacgtccacg cccgcgccgg acaggaagac ggtgtcgccc tgctcgatca
     3421 cgatcgccgc cggagcacgc ctggacaagc gcttgatggt gtcgtctatc gcggcgtccc
     3481 ccgcgggagc gtggtcgatg ctcaaaaagt tcgcctcggc gtcgcgcagc gaccgctcca
     3541 cgaagtcgtc gaagcggatg tcgtggacga ggaggtacgc gcccagccct accgtgccgg
     3601 cggcgacgcc ggcgacgagg acgaacgcca ccgtgagccg ccggcggaac tgtccggtct
     3661 ggacgggacg ctccaccgtc acccgtggtc tatgcggtag ccgaccccgc ggacggtctg
     3721 gatcaggcgg ttgccgcccg ccgcgggctc gaccttctcc cgcagtcgct tgaccgccat
     3781 gtcgacgacg cgcgagtccc ccaggtagtc gtagtcccag acgagctcga ggagcatctc
     3841 gcgggtcatg acctgtccgg cgtgacgcgc cagggcgacg aggagcttga actcggtcgc
     3901 cgtgaggtcg acgggggagc cggcgcggct gaccttgtgc ccggggacgt cgatgcggag
     3961 gtccccggcc gagatcacgt ggggctcggg tcctaccgac acccggcgga ggagcgcgcg
     4021 tacccgcgcc accagctccg gcatctcgaa cggtttggtg acgtagtcgt ctgcgccggc
     4081 ctcgagcccg agcacgacgt ccgcggtgtc ggccctggcc gtgagcatca ggatcggcac
     4141 ggggctgtgc gcccggatct cgcggcagac tccgagcccg tcgagcgacg gcagcatcac
     4201 gtcgagtacc acgaggtcga agtcctcttg cccgaagcgg acgagcgact gcctgccgtc
     4261 ctcttccttg gtgacgcgga acccggcttg tcccagcccc cgcgccgtga tctcgccgat
     4321 ggcagggtcg tcctcgacca gcaggacacg ctcgtccact cgtccgagga tactcagggt
     4381 gacgctcccg taggccattc gagcgattcc gcgcgtgcgc agagatcggc cgtgtctacg
     4441 acgcccacga ccgtcccgcg gtcgagcacg aacagcatcc gcacgtgcgc gagggcgatc
     4501 agtcgctgca cgtggaccgg gtcggcgttc acgtccacgt agagaggcgt cccgtcacgg
     4561 agcgcctccg gagacggcgc cgagacgagg tccacgccct gctccaggag gagggggccg
     4621 agctcgcgga gagtctctgc atccacgagg cccggagaca cgaggtcgat catgttcatg
     4681 gcatccagtg tgaggcgcgt cccgctctaa cgatgcttcg ttgcgtaacg atcccgcgac
     4741 gaacgacggc acccgggggg cctggggggc ccccgggtgc cgccggcgga gaggcgtgcc
     4801 ggggagggct tacctctcgc agctccgcag gtcgtctttg cccttgcctc cgcgtgcggt
     4861 gtcgcggccc ttcccgcagg cgagcgtgtc gtcgcccgcg ttgccgtgga gggtgtcgtt
     4921 ccccgatttg cccgtgagga tgtcgtcgtc gcctccgccg cggatcaggt ccgagtgcct
     4981 gccgccgtgg agattgtcga cgccggctcc gccacgcagg atgtccgcgg cgttccagcc
     5041 gtagagcgcg tcgttcccgg tgtgaccggt caggacgtct ctgccgctgc cgccgcgcag
     5101 atcgtcccat ccgtcgttgc cgcgcaacgt gtcgtgacct tcctggccgc ggagccggtc
     5161 gtgaccgctt ccgcccaccg cgacgtcgtc accggggccg aggaagacgt ggtcgtcacc
     5221 tccgcctccg tcgacgaagt cgtccccgcc gtacgtatag atgtactcgt gcgcggcggt
     5281 gccgacgatc cggtcgtcgc ccgggcttcc gcagatgacg ttggtgtcac cgcattccgg
     5341 tctgtcctgg gcctcggtga ccgggatgcc ccaggcgagc atggccacgg cggtcgcacc
     5401 cacggctccc aggcggctcc tcttcgtaag tcgcatctcg tacctcctcc tcgtcgagtg
     5461 gatcgttcgt tcccagcggt cctccccggc gggtgcgacg aaggggtcac ggcgaggtaa
     5521 caggtccgcg acagtggccg cggcgtcacg tcacgggcac ttgcgtttgt cccggcgagg
     5581 actgcgcgcg cgtatcgacg cggcagacac gagatcgtcg aatgcgaggg tcacttgagc
     5641 ggacgaggtc gcccgagggg cgttctcgtt catatggaag gcgaccacga gcagcgctgt
     5701 aagcggcgcc cccggtgagc cgcccggttc ccagcacgcg tatccagggg gttccgtcgg
     5761 gcctacacgt ccaccgggcc cacctttcga ccggctcggc ggcgaaaatg ccgtcctctg
     5821 acgcggcatc cagcgcggcg tgccccgagg ctttcgaagc acaagagggt ttcgcggcgc
     5881 ggccgacgaa tcggtggcgg gtccgagagc cggccgaccg cgaagggaac gggatgaacg
     5941 tgctgagaag aggtgtggcg gctgcggccg tcgtggggat gctccagctc gtcgccgccg
     6001 ggcccgcagc ggctaccggc accgcgacgt gcgttccgct gcccggctcg accccggcgg
     6061 tcggagcgga cctcgacggc gacggcaccg aagacgtgcg gctgcccgcg atctcggacg
     6121 tcgagctgtg cgccggtgcg aacgtcgtgc tgaccgagcc ggtcacgatc gagcgagagc
     6181 agtgcggcgg gttcggcagt tgcatgaggt actacgtgac ctacgaggtg agtggctacg
     6241 cacagaagga cctgacgttc tgctactcgg ccgacggcac gcgggtgtgc accgcgaccg
     6301 accccgcgcc gatcccggtg gacctcgtgt ccccacagac gatgtgcttc ggctgggacc
     6361 tgcgcggggg ctacccctgc ccgagcgggg ggccggtaga gctgggctag ccgcggtgcg
     6421 aaacgtcctc gccgcttatc tcgagggctg agcggccctt attacggcca ggtcacgcct
     6481 cgtccagacg acctctgcct cgaacccggc gcgcgcgagc cacgcgagct ggtccggcac
     6541 ggagctcggg aagtcgtacc ccggctcgtt ctcgatcacc gcgtcggcgg gatcgtcggg
     6601 gacgacgacg tcacccagca cgaagacgcc gcccggcgcg agcgccggcg cgatccgctc
     6661 gaacagcccg gtcttgtgct cgccttcgag gtgatgcacg gccagcgcgg agacgacgag
     6721 gtcgaacggc cccggcggca acgggtcgcc gaggtctgct tccacgaacg tcactcgatc
     6781 ggccgggagg atgcgggccg cgccggcgag catctcgggg ctcgagtcga cgccggtcag
     6841 atgcgcgccc tcgtgcacgg tgagcaggcg cgccgccgtc tcgccggcac cgacgccgag
     6901 ctcgaggatc gattgcgcgg caatgcctcg cgtggcgtcg acgacccgct cctgcagctc
     6961 gtcgtagtcc tcgatctcgg cacggatcgt gccgggatag gtgtccgggt cccaggtcat
     7021 cgccgcgcac gctagcagcc cggcgctacg ctccgacgat ggtgtccaac cggcgcgtct
     7081 ccttgaccgg cctgcgccgc gtgttcgccg ggtcgctggt cgcgatggcg ctcgtcgtcg
     7141 tgctgacgtg gttcgtgagc cgggaaggct cgaccgacgc tcccacgatg agcccccgca
     7201 caatcctgat cctgctcgcg gcctacggcg cgctcgacct cgtgctcgtc cgagctgcgc
     7261 ttcgcaggac gccgccgcag accgccaccg tcgaagcggt aggggactgg ttccgcgcgc
     7321 tgacgctgct cggccattcc ctcgggatgt cttcgttcct gatgggcttc gtgggggtct
     7381 tcctcgccaa cgagtggacg ctggtgctcg ccggcgtccc ggtcgcgctg atcgtgttct
     7441 ggctcgtggg gcccaccgcg cggcgcgtgg cgaacgctca gcgccggctg aactccgccg
     7501 gggtcgcggt cgagctcacg gacgcggtcg aggtgtcgcc tcagacggcc ggcggtcttg
     7561 cgcggtagcc gtaccggtgc aggaacggga acgcaaccgc cgtcgtgatc cagcggtcga
     7621 tccggctctg gccttcgcgc cagtccgacc tgtcctcgag caccagcgtc ccggtcgaga
     7681 aacgcgacgg gttcccggct atcgagtggt tcacgccgag cgtgacggtg cggtcgtcga
     7741 cgaaaggtga gcccgccgac gtctctccga ggagccgacg cgcctgctcg accgttgcac
     7801 gcggctgtgc cacgaagtcc tcgtaacgga ggcgcagggc gcgcccggcc cccgctctcc
     7861 gcagcacggc ctcggagccg aggttcagca ggtcccaccg caacgtcgcg tcgagcgcgg
     7921 tccggcg
//
LOCUS       DASQDO010000454         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_721212, whole genome shotgun sequence.
ACCESSION   DASQDO010000454 DASQDO010000000
VERSION     DASQDO010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721212"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="VEV43_12425"
     CDS             <1..2043
                     /locus_tag="VEV43_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HYP24371.1"
                     /translation="ECLQDVFDLPALVELMASVRRREVRVVEVDTPVPSPFASSLQFG
                     YVSAFMYEGDAPLAERRAQALSLDRSLLAELMGREELRELIDDAALAELEIDLQALGD
                     RGKATNEDGLHDLLRRLGDLSADEIAARSTGTTGAWVTALTERGRILPVRVAGHERFI
                     AAEDASRYRDALGAALPVGLPAAFLEPVDDPLLDLVARFARTHGPFTEEDVAQRFGIG
                     PAVARKTLAALEADGRVVEGEFRPGGSGREWIEVEVLRLLRRRSLAAFRKEVEPVAPE
                     VLARFTVAWHGIGPHGPRAATPDALLSVIEQLGGAPLPASALETRILPARLPGYSPAL
                     LDQLGASGEVVWCGAGPIGSTDGWVALALADRADLLLPEPVEAELSPPAAAALDALGS
                     GGAMFFRQIADAAGSTDDTELLLALWELVWAGRITNDTLGPLRALLRGGTRSSRPARA
                     RRGPLMPSRMGPPAAAGRWSLVPERRGDATRRLHAMAEQLLVRHGIVTRGAAMAERVV
                     GGFAGVYAVLKAFEESGRCRRGYFVEGLGGAQFAAPGAVDRLRSLEEPGDKAARTHVL
                     AAVDPANPYGAALEWPEREGTHRPGRKAGALVVLVDGRLAVYVEKGGRSLLSYSDDPD
                     VIGPAVDALALAARDGALGRLTVERADGEAIEDTPFAQALIDAGFRPTSRGLRLRA"
     gene            2036..2818
                     /locus_tag="VEV43_12430"
     CDS             2036..2818
                     /locus_tag="VEV43_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYP24372.1"
                     /translation="MPEGDTVFVAATRLHRALAGRTLTKTDFRVPAIATADLSGRQLL
                     EVASRGKHFLFRMEGGTTFHCHFKMEGEWHLYRPGSRPRGPAHAIRTILETDEWTAVG
                     FHLAMTELVPTTEEDRVVGHLGPDPLTNWDASEAVRRLRAQARRPIGEVLLDQTVIAG
                     PGNVYKCEICFLRGVDPWTPVGEVPDPEGVVDLTARLLQANRKTGTQVTTGDDRPGRQ
                     RWVYGRGNKPCRRCNTPIKRKGQDPGGERVTYWCPSCQPSLT"
     gene            complement(2778..>3593)
                     /locus_tag="VEV43_12435"
     CDS             complement(2778..>3593)
                     /locus_tag="VEV43_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24373.1"
                     /translation="GSAMVLAIGEATWGSVAGASITAVATVLAAVLGYRANRYGSRRF
                     SGGGSAAEGRDRARLVHANVPAIVYQRPRRWPWRAGMAILLAITVLGAVPFGYSMYAY
                     AASGFEPLWWFVGVVFAAPAAVALGWLIALFQIGFDRAGVHAQRVHVEGTVRTVMDQA
                     TEALSRMKIGVRKYEVSEDTGTIDASRVGRADNVYIRLEQLDDTTCEVDVKAESQPGI
                     FSFRKNSRYVAQFLSELTGVRVYPASFIPDGTRRSKGVTWLTSAKAGRRGTSR"
BASE COUNT          526 a         1261 c         1297 g          509 t
ORIGIN      
        1 gagtgcctgc aggacgtgtt cgacctgccc gcgctcgtcg agctgatggc gtccgtacgg
       61 cgacgagagg tgcgggtcgt cgaggtcgac acaccggtgc cgtcgccgtt cgcgagctcg
      121 ctccagttcg gctacgtgag cgcgttcatg tacgagggcg acgcgccgct ggcggaacgg
      181 cgcgcgcagg cgctttcgct cgaccgctcg ctgctcgccg agctcatggg ccgcgaggag
      241 ctccgcgagc tgatcgacga cgcggccctc gctgagctcg agatcgacct gcaggccctg
      301 ggcgaccgcg ggaaggcgac gaacgaggac gggctccacg acctcttgcg gaggctgggc
      361 gacctgagcg ccgacgagat cgcggctcgt tccacgggaa ccacgggggc gtgggtcacc
      421 gcgttgaccg agcggggccg gatcctgccg gtgagggtcg ccgggcacga acggttcatc
      481 gcggcggagg acgcatcgcg ctatcgcgac gccctggggg ccgcgctgcc ggtcggcctt
      541 ccggcggcct tcctcgagcc cgtcgacgac ccgctgttgg acctcgtcgc gcgcttcgca
      601 cgcacgcacg gacctttcac ggaagaagac gtcgctcaac ggttcgggat cgggccggcg
      661 gtggcgcgca agacgcttgc ggccctcgaa gcggacggcc gcgtggtcga aggagagttc
      721 cggcccggcg gcagcggacg ggagtggatc gaggtcgagg tcctgcggct cctccggcgg
      781 cgctcgctcg ccgcgttccg caaggaggtc gagccggtcg cgccggaggt cctcgcgcgc
      841 ttcacggtcg cgtggcacgg catcgggccg cacggccccc gcgccgcgac cccggacgcg
      901 ctgctttcgg tgatcgagca gctcgggggc gcgccgttgc cggcgtccgc gctcgagacg
      961 cggatcctcc cggcccgcct gcccggctac tcgccggcct tgctggacca gctaggagcg
     1021 tcgggtgagg tcgtgtggtg cggggccggg cctatcggga gcacggacgg ctgggtcgcg
     1081 ctcgcgctcg cggaccgagc ggacctgctg ctgccggagc cggtcgaagc ggagctctcg
     1141 cccccggctg cggcggcact cgacgcgctc ggctccggcg gcgcgatgtt cttccgccag
     1201 attgccgacg cggcggggag caccgacgac accgagctgc tgctcgcgct gtgggagctc
     1261 gtgtgggcgg gccggatcac gaacgacacg ctgggtccgt tgcgcgcgct cctgcgcggc
     1321 ggaacgagat cgtcgaggcc ggcgcgcgca cggcgcggcc ccctgatgcc gtcgcgcatg
     1381 ggacctcctg ccgcggcggg acggtggagc ctcgtgcccg agcgccgggg cgacgcgacg
     1441 cggcgcctgc atgcgatggc ggagcagctg ctcgtgcgcc atgggatcgt caccaggggg
     1501 gcggcgatgg ccgagcgggt cgtcggcggt ttcgcgggcg tctacgcggt gctgaaggct
     1561 ttcgaggaat cgggacggtg ccggcgcggc tacttcgtcg agggtctcgg cggcgctcag
     1621 ttcgcggcgc cgggcgcagt cgaccgcctg cgctccttgg aggagcccgg cgacaaggcg
     1681 gcccggaccc acgtgctcgc cgccgtcgac ccggccaacc cgtacggggc cgcgctcgag
     1741 tggcccgaac gcgagggaac ccatcgcccg ggccgcaagg cgggcgcgct cgtcgtgctc
     1801 gtcgacggac ggctcgccgt ctacgtcgag aagggcgggc gaagcctgct gtcgtactcg
     1861 gacgaccccg acgtgatcgg cccggcggtg gacgcgctcg cgctggctgc gagggacgga
     1921 gcgctggggc ggctcacggt cgagcgtgcg gacggtgagg cgatcgagga cacaccgttc
     1981 gcgcaggcac tgatcgacgc cggcttccgc ccgacgtcgc gcggcctgag gctccgtgcc
     2041 tgagggcgac accgtcttcg tcgcggcgac gcgactccac cgggcgctcg ccggccgcac
     2101 gctcacgaag accgacttcc gcgtgccggc catcgcgacg gccgacctct cggggcggca
     2161 gctcctcgaa gtggcgtcgc ggggcaagca cttcctcttc cgcatggagg gcgggacgac
     2221 gttccactgc catttcaaga tggaaggcga gtggcacctg taccgtcccg gctcccgccc
     2281 ccgcggcccc gcgcacgcga tcaggacgat ccttgagacc gacgagtgga cggcggtcgg
     2341 cttccacctg gcgatgaccg agctcgtccc gacgacggaa gaagaccggg tggtaggcca
     2401 cctcggcccg gacccgttga cgaattggga cgcctctgaa gccgtccgcc gtctccgcgc
     2461 gcaagcccgt cgcccgatag gagaggtact gctggaccag acggtgatag ccgggccggg
     2521 caacgtctac aagtgcgaga tctgcttcct gcgcggagtg gacccgtgga cgccggtagg
     2581 ggaggtgccg gatcccgagg gcgtggtcga cctcaccgcg aggctgctcc aagcgaaccg
     2641 caaaaccggc acccaggtaa cgacgggcga cgaccgcccc ggccgccaac gctgggtcta
     2701 cggccgaggc aacaagccct gccgccgctg caacacgccg atcaagcgaa agggccagga
     2761 ccccggcggc gagagggtca cctactggtg cccctcctgc cagccttcgc tgacgtgagc
     2821 caagtcaccc ccttgcttct tcgcgtcccg tccgggatga acgacgcggg gtacacacga
     2881 acccccgtga gctcgctgag gaactgcgcg acgtatcgac tgttcttcct gaagctgaag
     2941 atgccgggct gggattccgc tttgacgtcg acctcacagg tcgtgtcgtc gagttgctcg
     3001 aggcgtatgt agacgttgtc ggctcgaccg acccgagacg catcgatggt tcccgtatcc
     3061 tccgagacct cgtacttccg cacaccgatc ttcatccggc tcaaagcctc agtcgcttga
     3121 tccattacgg tccgaacggt gccctcgacg tgaaccctct gcgcgtgcac gcccgcccga
     3181 tcgaacccga tctgaaatag cgcgatgagc caccccagcg ccacggcagc gggggcggcg
     3241 aacacgacgc ccacgaacca ccacagcggt tcgaacccac tggcggcgta cgcatacatc
     3301 gagtacccga acggcacggc cccgagcacc gttatggcga gaaggatcgc catgcccgcc
     3361 cgccacggcc agcgacgagg tcgctgatag acgatcgcgg ggacgttcgc gtggacgagc
     3421 cgggcgcgat ctcgaccttc ggcagcgctg ccgcctccgg agaatcgccg acttccgtat
     3481 cggttggcgc gatacccgag gacggctgcc agcacggtcg caaccgcagt gatggatgcg
     3541 cccgcgaccg aaccccaagt cgcctcgccg atggctagca ccatcgcgct gcc
//
LOCUS       DASQDO010000455         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_476437, whole genome shotgun sequence.
ACCESSION   DASQDO010000455 DASQDO010000000
VERSION     DASQDO010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476437"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1352)
                     /locus_tag="VEV43_12440"
     CDS             complement(156..1352)
                     /locus_tag="VEV43_12440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HYP24374.1"
                     /translation="MKAVTWHGQRDVRVDEHPDPVIQKPDDAIVRITTTAICGSDLHL
                     YEMLNPYLDAGDILGHEPIGIVEEVGPDVTNIAPGDRVVIPFNISCGSCFMCSQSLYS
                     QCETTQVREYGMGAALFGYTKMYGQVPGGQAELLRVPFANFGPIKVPEGPPDERFVYL
                     SDVLPTAWQAVAYANVPKGGSVIVLGLGPIGDMCCRVARQHGAGQVIGVDMVPERIAR
                     VRALGFDVLDFEELGNGLTEAIRDMTDGRGPDSVIDAVGMEAHGSPGSTVAKGAQLMT
                     ALMPDKLAQKMMEHVGVDRLSALYSAIDIVRRGGTISLSGVYGGTADPLPMLTMFDKQ
                     IQLRMGQANVKRWVDDILPLVLDDEDPLGVEGFHSHQMPLEDAAYGYEIFQKKEDDAI
                     KVLLKT"
     gene            complement(1428..2645)
                     /locus_tag="VEV43_12445"
     CDS             complement(1428..2645)
                     /locus_tag="VEV43_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24375.1"
                     /translation="MSLLLIALLTWGGLAAPASGAAAIELSPGQAKFWNGPHLQSGAE
                     TWTYEIDVKKGGYRLRIGLDHAEVGDAYSFQVFGPDGQQEGTVNLGTGLYSGEFLQEG
                     PPAGRWRVEVSADSVTDSSFRMRAKLEARKPSLGTKKGPVLPNLQVLPPHEATFLMPV
                     TNGSQGDPPQGAPGDSCHPEERAQDGAIRCLRFAFGVRNTGRGPMHLYYEGGAPQPHP
                     LFQRVHRADGSFFDREAGTAVWHESHAHYHHGDAVAVELFAVTDREKGTLESAGPRHN
                     KGFAHRNELLREWRRFYPTWTGDLGFGLLPGWADIYEWDRPGNYVDFGVNGDGYYVVR
                     MWADPVDGILESNDRDNAGYTYFQVTGDQVELIEAGRGKHPWDPCKIVVGFGGHPDPK
                     RGTRPDRCPRDTT"
BASE COUNT          380 a          952 c          932 g          481 t
ORIGIN      
        1 gtcgcgctca cgctccccct ggtcgcgggc gtcttcgggc tggtcgaggt cttcggagcc
       61 gcggcctacg cagccggacg caacggcgcc ctggtcggtc tcgtcgaggc tctctgggtc
      121 gcgggcatcg tcgcgatcgc ggtgccgctc ccgggctagg tcttcagcag gactttgatc
      181 gcgtcgtcct ctttcttctg gaagatctcg tacccgtagg cagcatcctc gagcggcatc
      241 tggtgggagt ggaagccctc gacaccgagc gggtcctcgt cgtcgagcac gagcggcagg
      301 atgtcgtcga cccaccgctt cacgttcgcc tggcccatgc gcagctggat ctgcttgtcg
      361 aacatcgtga gcatcggcag cgggtcggcg gtgccgccgt acacgccgga gagcgagatc
      421 gtcccaccgc gacggacgat gtcgatcgcg ctgtagagcg cgctgaggcg gtcgacgccg
      481 acgtgctcca tcatcttctg cgcgagcttg tcgggcatca gcgcagtcat cagctgcgct
      541 cccttcgcta ccgtcgaacc gggagagccg tgggcctcca tgccgaccgc gtcgatgacg
      601 gagtccggcc cccgtccgtc ggtcatgtcg cggatcgcct cggtgagacc gttgcccagc
      661 tcctcgaagt cgaggacgtc gaagcccagc gcgcgcaccc gcgcgatccg ctccggcacc
      721 atgtcgactc cgatgacctg tcccgcgccg tgctggcgtg cgacgcggca gcacatgtcg
      781 ccgatcggac cgagcccgag cacgatgacg ctgccgcctt tcgggacgtt cgcgtaggcg
      841 acggcctgcc acgcggtcgg gagcacgtcg gagaggtaca cgaaccgctc gtcgggcggc
      901 ccctcgggca ccttgatcgg gccgaagttc gcgaacggca cgcgcaagag ctcggcctgt
      961 ccgcccggca cctggccgta catcttcgtg tagccgaaca gcgccgcgcc catcccgtac
     1021 tcgcgcacct gcgtcgtctc gcattgcgag tagaggctct ggctgcacat gaagcagctt
     1081 ccgcacgaga tgttgaaggg gatcacgacg cggtcgccgg gtgcgatgtt cgtgacgtcg
     1141 ggaccgacct cctcgacgat ccctatcggc tcgtgcccga ggatgtcgcc cgcgtcgagg
     1201 tacggattca gcatctcgta gaggtgtagg tccgagccgc agatcgccgt agtcgtgatg
     1261 cggacgatcg cgtcgtcggg cttctggatg accgggtcgg gatgctcgtc gaccctgacg
     1321 tcgcgctgcc cgtgccaggt gactgccttc atgttggttg acccctctcg cgtcgttggt
     1381 tagaggggcc ctacccgctc gggggcgggg cgatgcgtgc gcggacgcta ggtcgtgtcg
     1441 cgcgggcagc ggtcgggccg cgtccctctc ttcgggtcgg ggtgacctcc gaagccgacc
     1501 acgatcttgc acgggtccca cgggtgcttg ccgcggcccg cttcgatcag ctcgacctgg
     1561 tcgcccgtga cctggaagta cgtgtagccc gcgttgtcgc gatcgttgga ctcgaggatg
     1621 ccgtcgacgg gatcggccca catccgcacg acgtagtagc cgtcgccgtt gacgccgaag
     1681 tcgacgtagt tgccgggccg gtcccactcg tagatgtcgg cccagcctgg aagcaaaccg
     1741 aacccgaggt cgccggtcca cgtcgggtag aagcgcctcc actcgcgcag gagctcgttg
     1801 cggtgcgcga agcccttgtt gtgtcgcggg cccgccgact cgagcgtccc cttctcgcgg
     1861 tcggtcacgg cgaacagctc gaccgcgacc gcgtcaccgt ggtggtagtg ggcgtggctc
     1921 tcgtgccata cggccgtccc cgcctcgcgg tcgaagaacg agccgtcggc tcggtgcacc
     1981 cgctggaaca acggatgcgg ttgcggcgcg ccgccctcgt agtagaggtg catcggcccg
     2041 cgtccggtgt tgcgaacgcc gaacgcgaac ctcagacagc ggatcgcacc gtcctgcgcg
     2101 cgctcctcgg gatgacacga gtcgccggga gcaccttgcg gcgggtcgcc ctgggacccg
     2161 ttggtcaccg gcatgaggaa agtcgcctcg tgtggaggca gcacctggag gttcggcagc
     2221 accgggccct tcttcgtccc gagcgacggc ttgcgcgctt cgagcttcgc ccgcatccgg
     2281 aacgacgagt cggtcacgct gtcggcgctg acctcgacgc gccacctccc cgcgggcggc
     2341 ccctcttgga ggaactcgcc cgaatacagg cccgtgccga ggttgacggt cccttcctgc
     2401 tgtccgtcgg ggccgaacac ctggaacgag tacgcgtcgc cgacctcggc gtggtcgagc
     2461 ccgatcctca ggcggtagcc gcccttcttc acgtcgatct cgtacgtcca ggtctcggcg
     2521 ccgctctgga ggtgggggcc gttccagaac ttcgcctgtc ccggcgagag ctcgatcgcg
     2581 gccgcgcccg acgccggggc cgcgaggccg ccccacgtca ggagcgcaat cagcaggagg
     2641 ctcatccttc tcatcctcgg acatctttac gcatccaccc cgcccggtcc tgcggggtgc
     2701 ccgaagtttc acgggggcgg gtgggggtag tcgcccaacg atccg
//
LOCUS       DASQDO010000456         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_232012, whole genome shotgun sequence.
ACCESSION   DASQDO010000456 DASQDO010000000
VERSION     DASQDO010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232012"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VEV43_12450"
     CDS             <1..283
                     /locus_tag="VEV43_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYP24376.1"
                     /translation="GYYDDGILRYAGKVGTGFTQKTLADLHARMKPLEQTESPFEGRP
                     PVRKGAHWLKPKLVAQIGFSEWTTDAKLRHPRFLGLRTDKKAREVVREV"
     gene            285..3002
                     /gene="malQ"
                     /locus_tag="VEV43_12455"
     CDS             285..3002
                     /gene="malQ"
                     /locus_tag="VEV43_12455"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYP24377.1"
                     /translation="MPDPRAWGVAPGYMDAREQWKDAPPETIAAVLDAMGADGDAPPE
                     SPVVVARPGERVDLGDAVEIVFEAGGSAPAGAVLPPDTPLGYHRVVGRDGGERDLIVS
                     PGACHLPKGLRAWGWAVQLYSARSSRSWGMGDLGDLAELGRWGRGTGADVLLVNPLHA
                     PQPGPQQPSPYFPSSRRWRNPLYVRIEDVPGAAEQLDDVADLASQGRKLNAGDRIDRD
                     EVYRLKMEALERLWRSRADDAEFHRFRERHGNSLTDFATYMTLVEHHGGGPPSWPAKF
                     RDPARPAVKKFRVDNEERVLFHEWVQWLLERQLLGAADEIGLVHDLAIGVDPEGADAW
                     LWADVFADGVTVGAPPDLFNSHGQNWGLPPFDPWKLRAASYRPFVETLRSNLVGAAGL
                     RVDHVLGLFRLFWIPEGAGPVDGTYVRYPYGDLLDVLALESHRAEAFIVGEDLGTVED
                     VVPEEMQARHMLSYRLLWFDERPDEYPERSLAAITNHDLPTVTGMWTGEDLQEQRDLG
                     LDPNEEAEADVRERFRKAAKLRDDAAPADAVKKAHSLLARASSAVVVATLEDALEVRR
                     RPNQPGTTDERPNWSMPLPVPIDELAERPLVKSVARTLARGRRSALPRPELADTEIRH
                     GYAQLSEVKLHYAEAGSGPLVVMLHGYPEFWYSWRRQIPFLAERGFHVVAPDMRGYNL
                     SDKPSGIGPYRIQSLVRDLRELVEHFDGGPSRPAHVVGHDWGGIVGWYFAMSHPLLLD
                     RLTIMNAPHPQRYVRAMNPRQMTRSWYVGFFQLPWLPERLMAANDYEALRRVFRHDPS
                     RGDAFTDEDVERYVEAARRSDGLRYPINYYRALLRPNPLPALRWRVIERPVQVIWGER
                     DRALGPELAEPDRAWVPDVEVHRLPEATHWVQHDAPARVNELLADFLAG"
     gene            complement(3004..3756)
                     /locus_tag="VEV43_12460"
     CDS             complement(3004..3756)
                     /locus_tag="VEV43_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24378.1"
                     /translation="MALRDVAEVVTEFGIDQARIIGGHMVMLHVYRHGLGSHLYRATA
                     DADLGVPPIAVKEAGVIEALESRGYTRHGGNRYRRPVDEAADVVDEPYAVVDILVPAY
                     TSRPRENRVVSPGLTTVEVPGLALALGRPAVEVRLDVALSAGAALAIDVSLPDEASSF
                     VMKALAWERRGARKDIVDVWRMLEVLVATSPSQEIPAPEAVEAVRTAFRDGRAAGTRA
                     LVAELSLGDAAARERWTRIQALMLRCLPEVGR"
     gene            complement(3795..4865)
                     /locus_tag="VEV43_12465"
     CDS             complement(3795..4865)
                     /locus_tag="VEV43_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24379.1"
                     /translation="MITVSTVLTLGIVLTKMIDSLQEDGISVSFVEVPDDLPDAVLEI
                     YADGATRRLAIDRRRRAPYPGELGTLFEHRDALGRWGTPTLVAPFIPESVARLLIPLG
                     WSWADAQGNYDLTLGRGMRFKQRVSSSPPRRSPQRSLPQGSGGLSIVRFLISTHRPAH
                     HRTGELAKRFDITHARASQVLNQLMKANLVEKDSWGWSADRDALLQRFMEEYRGPGGT
                     EIALYSLDPPRDALETLLWNYRMPAAPHADRDVAVSADIGLDLVAPWHHPTVVVVYAR
                     ERSKLNLAAFVQADRASANLIVRYPADTSVFGAEPWLASLNERPLPLADPTQLIWDLH
                     DLGGEHRAEAAGHLSEWLLKRR"
     gene            complement(4914..5102)
                     /locus_tag="VEV43_12470"
     CDS             complement(4914..5102)
                     /locus_tag="VEV43_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24380.1"
                     /translation="MAEDREDQGGTTESTERIPPGSMPPDDDESSDDVPGGEGAEDES
                     KADEESKQSFPASDPPAW"
     gene            complement(5136..>5916)
                     /locus_tag="VEV43_12475"
     CDS             complement(5136..>5916)
                     /locus_tag="VEV43_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24381.1"
                     /translation="ILGLSGDDTIDGAGGDDLLEANYGDDTTRGGDGNDTIYDFGGRD
                     AVFGDAGDDLFFAGLNDDAYDGGAGTDTLDYFDAKKKVFADLGAGTADGMGIDTITGV
                     ENLSGGIKPDFLTGDAGPNVLVGRDGVDQLSGGDGDDVLDGGTFDDVLEGGNGSDMAT
                     YALVQTPVTADLEAGFATGQLSGSDNLLAIEGIEGSAATDFLRGDEGPNTFLGGPGGD
                     VLEGKGGDDVLDGGLGNDYLDGGSETDTCREGETTVNCESP"
BASE COUNT          890 a         2083 c         2031 g          912 t
ORIGIN      
        1 cggctactac gacgacggga tcctccgcta cgccggcaag gtcgggacgg gcttcacgca
       61 gaagaccctc gccgacctcc acgcgcggat gaagccactc gagcagaccg agtcgccgtt
      121 cgagggacgg cccccggtcc gcaagggcgc gcactggctg aagccgaagc tcgtcgcgca
      181 gatcgggttc tccgaatgga cgaccgacgc gaagttgcgc cacccgcgtt tcctcggtct
      241 ccgaacggac aagaaagccc gagaagtcgt gcgggaggtc tgagttgccc gatccgagag
      301 cttggggggt cgcccccgga tacatggacg cgcgcgagca gtggaaggac gctccgcccg
      361 agaccatcgc cgcggtcctg gacgcgatgg gggccgacgg cgacgcgccg ccggaatcgc
      421 cggtcgtcgt cgcgcggccg ggcgagcgcg tcgatctcgg cgatgctgtc gagatcgtgt
      481 tcgaggccgg gggctccgcg ccggccggcg ccgtgctccc acccgatacg ccgctcggct
      541 accaccgcgt cgtcgggcgc gacggcggcg aacgcgacct gatcgtcagc ccgggcgcgt
      601 gccatctccc gaagggcctg cgggcgtggg ggtgggcggt ccagctgtac tcagcacggt
      661 cgagccggag ctggggcatg ggggacctcg gggacctcgc cgagctcggc cgctggggcc
      721 gcgggaccgg cgcggacgtc ttgctcgtca acccgctaca cgcccctcag cccggccccc
      781 agcagcccag tccctacttc ccgtcgagcc gccggtggcg caacccgctg tacgtgcgga
      841 tcgaggacgt gcccggggcg gccgagcagc tcgacgacgt cgccgacctc gcgtcgcagg
      901 gccgaaagct caacgcgggc gaccggatcg accgcgacga ggtctaccgg ctgaagatgg
      961 aggccctcga acggctgtgg aggtcgcggg ccgacgacgc cgagttccac cgcttccgcg
     1021 agcggcacgg caactcgctc acggacttcg ccacgtacat gacgctcgtc gagcaccacg
     1081 gcgggggccc accgtcgtgg ccggcgaagt tccgcgaccc cgcgcggccc gccgtgaaga
     1141 agttccgcgt cgacaacgaa gagcgcgtgc tgttccacga atgggtgcag tggctgctcg
     1201 aacgccagct gctcggcgcg gcggacgaga tcgggctcgt ccacgacctc gccatcggcg
     1261 tagaccccga aggtgcggac gcgtggctgt gggccgacgt cttcgcggac ggcgtgaccg
     1321 tcggcgcacc gccggacctc ttcaactcgc acgggcagaa ctggggcctt ccgccgttcg
     1381 acccgtggaa gctccgagcc gcgagctacc ggccgttcgt cgagaccctg cgctcgaacc
     1441 tcgtcggcgc ggcgggcctg cgggtcgacc acgtcctggg gctgttccgg ctgttctgga
     1501 tccccgaggg ggccgggccc gtcgacggga cctacgtccg atatccgtac ggcgacctgc
     1561 tcgacgtcct cgcgctcgag agccaccgcg cggaggcgtt catcgtgggc gaggacctgg
     1621 ggacggtcga ggacgtggtc cccgaggaga tgcaggcacg acacatgctg tcgtaccgcc
     1681 tgctgtggtt cgacgagcga ccggacgagt accccgagcg gtcgcttgcc gcgatcacta
     1741 accacgatct ccccacggtc accgggatgt ggaccggcga ggacttacag gagcagcgcg
     1801 acctcggcct ggacccgaac gaagaagccg aagccgacgt gcgcgagcgt ttccgcaagg
     1861 cggcgaagct gcgcgacgac gcggcccccg cggacgcggt gaagaaggcg cactcgctgc
     1921 tggcccgcgc gtcgagcgcg gtcgtggtcg cgacgctcga ggacgctctc gaggtgcggc
     1981 ggcggccgaa ccagccgggg acgaccgacg agcggccgaa ctggtcgatg ccgctgccgg
     2041 tgccgatcga cgagctcgcg gagcggccgc tggtcaagag cgtggcgcgc acgctggcgc
     2101 ggggccggcg cagcgcactg ccgcgtcccg agctcgccga cacggagatc cgtcacggct
     2161 acgcgcagct gtcggaggtg aagctccact acgcggaggc cggcagcggg ccgctcgtcg
     2221 tcatgctgca cggctacccc gagttctggt actcgtggcg ccggcagatc ccgttcctcg
     2281 ccgagcgcgg gttccacgtc gtcgcgccgg acatgcgcgg ctacaacctc tcggacaagc
     2341 cttcgggcat cgggccgtac cggatccaga gcctcgtgcg cgacctgcgc gagctcgtcg
     2401 agcacttcga cggaggcccc tcccgccccg cgcacgtggt cgggcacgac tgggggggca
     2461 tcgtcgggtg gtacttcgcg atgagccatc ccctgctgct cgaccggctc acgatcatga
     2521 acgcgccgca cccgcagcgc tacgtccgcg cgatgaaccc gcgccagatg acgaggagct
     2581 ggtacgtcgg gttcttccag ctcccgtggc tgcccgaacg gctgatggcg gcgaacgact
     2641 acgaggccct gcggagggtg ttccgccacg atccctcccg cggcgacgcg ttcacggacg
     2701 aggacgtcga gcgttacgtg gaggccgccc gtcgcagcga cgggctccgt tacccgatca
     2761 actactaccg cgcgctgctg cggccgaacc cgttgcccgc gctgcgctgg cgggtgatcg
     2821 agcgcccggt gcaagtgatc tggggcgaac gggatcgagc gctgggcccc gagctggcgg
     2881 agccggaccg agcctgggtc ccggacgtag aggtccaccg cctgccggag gcgacgcact
     2941 gggtgcagca cgacgccccg gcccgagtga acgagctgct ggcggacttc ctagcggggt
     3001 gatctaccgc ccgacctccg gaaggcatcg caacatcagg gcctgaatac gcgtccaacg
     3061 ttcgcgtgcc gccgcgtcgc ccagtgacag ttctgctacg agcgcacgcg tgcctgcggc
     3121 gcgtccgtcc cggaaggccg tccttacggc ctctactgct tccggcgctg gaatctcctg
     3181 cgaaggcgaa gtcgcgacga ggacctcgag catccgccac acgtccacga tgtccttccg
     3241 cgcgcctcgt ctctcccatg ccagtgcctt catgacgaag ctcgacgctt cgtcgggaag
     3301 actcacgtcg atcgccaggg ccgcccccgc ggacagcgcg acgtcgagtc gaacttcgac
     3361 tgcaggacga cccagcgcga gcgcgagacc cggcacttcg accgtcgtga ggcccgggct
     3421 gacgactctg ttctcgcgcg gccggctcgt atacgcggga accaggatgt ctacgacggc
     3481 gtatggctcg tcgacgacgt ccgctgcctc gtcgacggga cgccgatagc ggttaccgcc
     3541 atgcctcgtg tagccgcggc tctcgagcgc ctcgatcacg ccggcctctt tgacggcgat
     3601 cggtgggacg ccgaggtccg catcggccgt cgctcgatag aggtggcttc ccagcccgtg
     3661 acggtagacg tgcagcatca ccatgtggcc gccgatgatt cgagcttggt cgatcccgaa
     3721 ctccgtcacg acctcggcga cgtcccgcag cgccaagaac gagagatcct cggcgatgga
     3781 gcgcgacgct atggtcagcg tcgtttcagg agccattccg aaagatgtcc cgctgcctca
     3841 gcgcggtgtt cacctcccag gtcatggagg tcccagatca gttgcgtggg gtcggcgaga
     3901 ggtagtggcc tctcgttcag tgacgcaagc cagggctcgg ccccgaacac ggaggtgtcc
     3961 gcgggatagc ggacgatcag gttcgccgat gcgcgatccg cctgcacgaa agcagcgaga
     4021 ttcagcttcg acctctcacg ggcgtatacg acgacgacgg tcgggtggtg ccacggggca
     4081 acgaggtcca gtcctatgtc ggcggagacg gctacatcac ggtccgcgtg cggggccgcg
     4141 ggcattcggt agttccatag gagcgtctcg agagcatccc gcggaggatc gaggctgtag
     4201 agcgcgatct cggttccacc gggccccctg tactcctcca tgaaacgctg cagcaacgcg
     4261 tcgcggtccg cgctccatcc ccagctgtct ttctcgacga ggttcgcctt catcaactgg
     4321 ttcaggacct gagaggcccg tgcgtgcgtg atgtcgaaac gctttgccag ctcacccgtt
     4381 cggtggtgcg cgggccggtg cgtcgagatc aggaaccgga cgatcgaaag gccaccgctt
     4441 ccctggggga gcgacctctg aggcgaccgg cgcgggggag atgacgagac gcgttgcttg
     4501 aaccgcatcc cacgcccgag cgtcaggtcg tagttgccct gcgcgtctgc ccagctccac
     4561 cccagaggga tgaggaggcg tgccacggat tcggggatga agggcgcgac gagcgtcggg
     4621 gtcccccatc ggcccaaggc gtctcgatgc tcgaagagcg tccccagctc gcccggatac
     4681 ggggcgcggc ggcgccggtc gatcgcaaga cgccgcgtcg ccccgtcggc gtagatctcc
     4741 agcaccgcgt cgggcagatc gtcgggcacc tcgacgaagc tgacggagat cccgtcttcc
     4801 tggagcgaat cgatcatctt tgtaagcaca attcctagtg taagcactgt gcttacagta
     4861 atcaagaggc ttacatcaga cggcccctgc gggcatagct actgagaccg cggctaccag
     4921 gccggcgggt cgctcgccgg gaaggactgc ttcgactcct cgtccgcctt ggactcgtcc
     4981 tcggccccct cgccacccgg cacgtcgtcc gacgactcgt cgtcgtcggg cggcatcgag
     5041 cccggcggga tccgctccgt cgattccgtc gtcccgccct ggtcctctcg gtcctcggcc
     5101 atgccccctc cttggtgtgt cggctccgag agaccctacg gggattcgca gttcaccgtc
     5161 gtctcgccct cgcggcacgt gtcggtctcg gaccccccgt cgaggtagtc gttgccgagc
     5221 cctccgtcga gcacgtcgtc gccgcccttg ccctcgagca cgtcaccgcc ggggccgccg
     5281 aggaacgtgt tcggcccctc gtcgccgcgc aggaagtcgg tcgcggccga gccctcgatc
     5341 ccctcgatcg ccagcaggtt gtccgagccc gagagctgtc cggtcgcgaa gcccgcttcg
     5401 aggtcggccg tcaccggcgt ctgcacgagc gcgtacgtcg ccatgtcgct gccgttgccg
     5461 ccctcgagga cgtcgtcgaa ggtcccgccg tcgaggacgt cgtcgccgtc gccacccgac
     5521 agctggtcga cgccgtcgcg gcccaccagg acgttcggcc cggcgtctcc cgtgaggaag
     5581 tccggcttga tcccgccgga gaggttctcg accccggtga tcgtgtcgat ccccatcccg
     5641 tcggcggtcc cggccccgag gtcggcgaac accttcttct tcgcgtcgaa gtagtcgagc
     5701 gtgtcggtcc ccgcgccgcc gtcgtaggcg tcgtcgttga gcccggcgaa gaagaggtcg
     5761 tcacccgcgt ccccgaacac cgcgtcgcgg cccccgaagt cgtagatcgt gtcgttcccg
     5821 tcgccgccgc gagtcgtgtc gtctccgtag ttcgcctcga ggaggtcgtc accgccggct
     5881 ccgtcgatcg tgtcgtcacc gctgaggccg aggatg
//
LOCUS       DASQDO010000457         4725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_294048, whole genome shotgun sequence.
ACCESSION   DASQDO010000457 DASQDO010000000
VERSION     DASQDO010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294048"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VEV43_12480"
     CDS             <1..1217
                     /locus_tag="VEV43_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HYP24382.1"
                     /translation="VALAETVATEVRAAGAAERCLAEASEDERTRERTADLLRYEISE
                     IDGAALRPGEVAELEAEATRLENAGALAEGIAEAESLLRGEGGAAESLATAQRVLEKL
                     AAHDPALGALAARLASITYDVADAGDEVAGREVAPDPDALESTRSRLQLIRALARKYG
                     DDEDAILEYREGAARRLAELDNADATLAALRDEVAAHRAAATEAATRLTELRRAAAPR
                     LEEGVEALLGTLAMGGADFRVALETADLYEGGAETVEMLVSANPGEVPRPVAKVASGG
                     ELSRISLALHLLTSAGSVTTTVFDEVDAGVGGEAAQSIGRALAKLARTTGAQVLVVTH
                     LPQVAAFADHQVVVTKDAEPSRTDAHVTLVAGDARLEELSRMLAGMRHSERARDHARE
                     LLDLAQAETVGA"
     gene            1214..2416
                     /gene="steA"
                     /locus_tag="VEV43_12485"
     CDS             1214..2416
                     /gene="steA"
                     /locus_tag="VEV43_12485"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HYP24383.1"
                     /translation="MKLAFKRRPSRPEPPREAGAIAATARVDKRTKNLVARMLPGEIA
                     VIDHEDVDRVSAEGLVQRKVAAVVNASRSSTGRYPNLGPLLLVSAGIALIDDAGPAVM
                     AVPEGTRLVVEGGRVLRETPGGSVEVATGKRLELEDVEHALDHAKQSIAGEIERFAEN
                     TISYIKDERDVLLEATRLPEVATDFHGRHVLIVVRGYDYKEDLVALRSYVREMRPILV
                     GVDGGADALLDNGMVPDMIIGDMDSVTTEALLSGAELVVHAYPGGRAPGLERLEALGL
                     ESVTFEATGTSEDIAMLLAFEHAAELIVAVGTHANLIEFLDKGRKGMASTFLVRLRVG
                     PILVDAKGVSRLYRGRVKRGDLALLVGAAAVTMAIVVTLSEPVRLELELMWAQLGNLW
                     FSIRQAIF"
     gene            2419..3333
                     /locus_tag="VEV43_12490"
     CDS             2419..3333
                     /locus_tag="VEV43_12490"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HYP24384.1"
                     /translation="MVDFRYHLVSIAAVFLALGIGLLMGSGVVGEPLLDNIRDRARDV
                     QEFNDRLKDDVDNLEHDLGVSRRFTEAVEPMLVNGRLAGEDVVIVDIASGDVPLDALL
                     ESLEDGAGARVASIVTLTDDFELDAEEDVTELGTITGSGATVDELRVEAARQVGAEIA
                     ALAEGRRGAEGEGLLPALEEAGFVDLAEEEEARPVPPGASVVIAASGVTDPTFRVDQV
                     ATALGAAAAESAPVVVVETTDSAWNIVGSVRGDGDLANLISTVDNVDKLSGRIALVMT
                     LDRLEGDGPGHFGEKSDVPVIPEPGPRD"
     gene            3338..>4725
                     /gene="murJ"
                     /locus_tag="VEV43_12495"
     CDS             3338..>4725
                     /gene="murJ"
                     /locus_tag="VEV43_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYP24385.1"
                     /translation="MTSTGERSLARGAAVITLATALSRVTGFVRVVVVAGAMGTTFLA
                     NTYQTANTAPNVIFELVAAGVLTSIFVPTFVEYIVRGEQEEGWEAGNALASVALVGLA
                     AIALVLAFAAPVVMRLLTIGVEDPVLRAREVELGATFLRLFAPQVAFYGAGMIMTGAL
                     HAHRRFALPAVAPLLNNLVVIGVYLTYAAMRAGRPPSVASITTAETLVLGIGTTLGVV
                     AMTLCLIPQLVRMGWRFRFRFDLSHPAVRKGARLGAWALGYAGGYQAGLIVVLMLANK
                     VEGGVAAYQWAYTFFYLPHALFAVPIFSVLFTAMSEHVARSEPAGVQEKLRDGLGMLV
                     FVLAPVGVALLVLSGPLATLARRYGAMTEAGAELVARCLGAFALGLPAYSIFLVFTRA
                     FYAIGDTKMPALVNAGAVAAASVAGAVFFAAAPEGWEVPGLAAGHSAGFALGAAILSG
                     AFARRAGRVRST"
BASE COUNT          637 a         1653 c         1761 g          674 t
ORIGIN      
        1 ccgtcgcgct cgccgagacc gtcgcgaccg aggtccgggc tgcgggggcg gcggagcgat
       61 gtctcgcgga ggcgtccgag gacgagcgga cccgcgagcg gaccgcggac ctccttcgtt
      121 acgagatctc cgagatcgac ggcgccgcgt tgcggccggg cgaggtcgcc gagctcgaag
      181 cggaggcgac ccggctcgag aacgcgggcg cgctcgcgga agggatcgcc gaggccgagt
      241 ctcttctccg gggggagggg ggcgcggccg agtctctggc gaccgcgcag cgcgtgctcg
      301 agaagctcgc cgcgcacgac cccgctctgg gcgcgctcgc cgcgaggctg gcgtcgatca
      361 cctacgacgt ggcggatgcc ggcgacgagg tagcggggcg cgaggtcgct ccggatcccg
      421 acgcgctcga atcgacgcgg tcgcgcctcc agctgatccg cgcgctcgcg cgcaagtacg
      481 gcgacgacga ggacgcgatc ctcgagtacc gcgagggggc cgcgcgccgg ctcgccgagc
      541 tcgacaacgc cgacgcgacg ctcgccgcac tccgggacga ggtcgcggcc caccgtgccg
      601 cggcgaccga agccgcgacg cgcctcaccg agctccgccg ggcggcggcc ccgcggctgg
      661 aggaaggggt cgaagccctc ctggggacgc tcgcgatggg gggcgcggac ttccgggtcg
      721 cgctcgaaac ggccgacctc tacgagggcg gagccgagac cgtcgagatg ctcgtgtccg
      781 cgaaccccgg cgaggtcccg cggccggtcg ccaaggtcgc ctccggcggc gagctgtcgc
      841 gcatctctct cgcgctccat ctgctgacgt cggcgggctc ggtcaccacg acggtcttcg
      901 acgaggtcga cgcgggcgtc ggcggcgagg ccgcgcagtc gatcgggcgc gcgctcgcca
      961 agctcgcacg gacgaccggc gcgcaggtgc tggtcgtcac gcacctcccg caggtcgccg
     1021 ctttcgccga ccaccaggtc gtcgtgacga aggacgcaga gccgtcgcgc accgacgcgc
     1081 acgtgaccct cgtcgccgga gacgcccggc tggaagagct gtcgcggatg ctcgccggga
     1141 tgcgccacag cgagcgcgcc cgcgaccacg cgagggagct gctcgacctc gcgcaggccg
     1201 agacggtggg cgcgtgaagc tcgccttcaa gcggcggccg tcgcgccccg agcccccgcg
     1261 cgaagccggc gcgatcgccg cgaccgcccg cgtcgacaag cggacgaaga acctggtggc
     1321 gcgcatgctc cccggcgaga tcgccgtgat cgaccacgag gacgtcgacc gcgtctccgc
     1381 cgaagggctc gtccagcgaa aggtcgccgc cgtcgtgaac gcgagccgtt cttcgaccgg
     1441 ccggtacccg aacctcggcc ccctcctgct cgttagcgcg ggcatcgcct tgatcgacga
     1501 cgccggaccg gcggtgatgg ccgtccccga gggtacgcga ctggtcgtcg aggggggccg
     1561 cgtgctgcgc gagacccccg gcggctccgt cgaggtcgcg accggcaaac gcctcgagct
     1621 cgaggacgtc gagcatgcgc tcgaccacgc caagcagtcg atcgccggcg agatcgagcg
     1681 cttcgccgag aacacgatct cgtacatcaa ggacgagcgc gacgtgctgc tcgaggccac
     1741 gcggcttccg gaggtcgcga ccgacttcca cggccgtcac gtcctcatcg tcgtgcgcgg
     1801 ctacgactac aaggaggacc tcgtcgcgct gcggtcgtac gtgcgcgaga tgcggccgat
     1861 cctcgtcggg gtcgacggcg gggcggacgc gctcctcgac aacggcatgg ttcccgacat
     1921 gatcatcggc gacatggact cggtgacgac ggaggcgctg ctcagcggtg ccgagctcgt
     1981 cgtccacgca tatcccggcg ggcgagcgcc cgggctcgaa cggctcgagg ccctcgggct
     2041 cgaatccgtg acgttcgagg cgaccggcac gtccgaggac atcgcgatgc tgctcgcgtt
     2101 cgagcacgcg gccgagctga tcgtcgccgt cggaacgcac gcgaacctga tcgagttcct
     2161 cgacaagggc cgcaagggga tggcgtccac gttcctcgtg aggctgaggg tgggcccgat
     2221 cctcgtcgac gcgaagggcg tgagcaggct ctaccgtggg cgcgtcaagc gcggggacct
     2281 cgcgctcctg gtcggcgcgg ctgccgtgac gatggcgatc gtcgtgacgc tgtccgagcc
     2341 ggtgcggctc gagctcgagc tgatgtgggc ccagctcggc aacctctggt tctcgatccg
     2401 ccaggcgatc ttctaaccgt ggtcgacttc cgctaccacc tcgtctcgat cgccgccgtc
     2461 ttcctcgcgc tcgggatcgg gctgctcatg gggtcgggcg tcgtcggcga gccgttgctc
     2521 gacaacatcc gcgaccgtgc ccgcgacgtg caggagttca acgaccgcct gaaggacgac
     2581 gtcgacaacc tcgaacacga cctgggggtc tcgcgccggt tcacggaggc ggtcgagccg
     2641 atgctcgtga acgggcgcct cgccggggag gacgtcgtga tcgtcgacat cgcatccggc
     2701 gacgtgccgc tggacgcgct gctggaatcg ctcgaggacg gggccggggc gagggtcgcg
     2761 tcgatcgtca cgctcaccga cgacttcgag ctggacgccg aggaggacgt gaccgagctc
     2821 gggacgatca ccggcagcgg cgcgaccgtg gacgagctga gggtcgaggc cgccaggcag
     2881 gtgggggccg agatcgcggc gctggccgag ggccgccgcg gcgccgaagg cgaggggctc
     2941 cttcccgcgc tcgaggaggc ggggttcgtc gacctcgccg aggaagagga ggcccggccg
     3001 gtgccgccgg gcgccagcgt cgtgatcgcc gcgagcggcg tgaccgaccc gaccttccgg
     3061 gtcgaccagg tcgcgaccgc gctgggggcg gcggcggccg agagcgcacc ggtggtcgtc
     3121 gtcgagacga ccgacagcgc gtggaacatc gtcggctcgg taaggggcga cggagacctg
     3181 gcgaacctga tctcgacggt cgacaacgtg gacaagctct ccgggaggat cgccctcgtc
     3241 atgacgctcg accgcctcga gggggacggg ccggggcact tcggcgagaa gagcgacgtg
     3301 ccggtcatcc ccgagccggg acccagagac tgacggcttg acgtccacag gggaacgctc
     3361 gctcgcccgc ggcgcggcgg tgatcacgct cgccaccgcg ctgtcgcgcg tcacgggctt
     3421 cgtgcgggtc gtggtcgtcg ccggcgcgat ggggacgacg ttcctcgcga acacgtacca
     3481 gacggcgaac acggccccca acgtcatttt cgagctcgtc gctgcagggg tgctcacttc
     3541 gatattcgtc ccgacgttcg tcgagtacat cgtgcgcggc gagcaagagg agggctggga
     3601 ggccggcaac gcgctcgcgt cggtggctct cgtcggcctc gccgcgatcg cgctcgtgct
     3661 cgcgttcgcg gcccccgtcg tcatgcgcct gctgacgatc ggcgtcgagg acccggtgct
     3721 gagggcgcgg gaggtcgagc tcggggcgac gttcctgcgc ctgttcgcgc cccaggtcgc
     3781 gttctacggc gcgggcatga tcatgaccgg cgcgctgcac gcgcaccgcc gattcgcgct
     3841 gcccgcggtg gcgccgctgc tcaacaacct cgtcgtgatc ggcgtctacc tgacgtacgc
     3901 ggcgatgcgg gcgggacggc ctccttcggt tgcgagcatc accaccgcgg agacgctcgt
     3961 gctcgggatc gggacgaccc tgggcgtcgt cgccatgacg ctgtgcctca tccctcagct
     4021 cgtccggatg gggtggaggt tccgcttccg cttcgacctc tcgcatcccg cggtgcgcaa
     4081 aggggcgagg ctcggggcct gggccctcgg ctacgccggc gggtaccagg cggggttgat
     4141 cgtcgtcctc atgctggcga acaaggtcga gggcggggtc gccgcgtacc agtgggcgta
     4201 cacgttcttc tacctgccgc acgcgctgtt cgccgtgccg atcttctcgg tcctgttcac
     4261 ggcgatgtcc gagcacgtcg cgcgcagcga gcccgcgggc gtccaggaga agctgcgcga
     4321 cggcctcggc atgctcgtgt tcgtgctcgc gcccgtcggc gtcgcgctcc tcgtcctgtc
     4381 ggggccgctc gcgacgctgg cgcggagata cggcgcgatg accgaggcgg gggccgagct
     4441 cgtagcgcgc tgtctcggcg cgttcgccct gggcctcccc gcttattcga tcttcctcgt
     4501 gttcacccgc gcgttctacg cgatcggcga tacgaagatg cccgcgctcg tcaacgccgg
     4561 cgccgtcgcg gccgcgagcg tcgcaggcgc cgtcttcttc gcggccgcgc ccgaggggtg
     4621 ggaggtaccg gggctcgccg ccggtcactc cgccggcttc gcgctggggg ccgcgatcct
     4681 gtcgggggcg ttcgcgcggc gggccgggcg cgtgcggtcg acgga
//
LOCUS       DASQDO010000458         7679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1027594, whole genome shotgun sequence.
ACCESSION   DASQDO010000458 DASQDO010000000
VERSION     DASQDO010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027594"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VEV43_12500"
     CDS             <1..623
                     /locus_tag="VEV43_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP24386.1"
                     /translation="MDELPLFRRDVLEALRGPLEDGVVQVVRSGGSVTFPCRFSLVAA
                     MNPCPCGYGEEGALCPCSPSAVRAYRARISGPLMDRFDLHLTMTRLRAADLMREREGE
                     PSATIRERVEHARSIQRERYRAAGATNAAVSKRTLLLAGRFRPEAYEQLHGAIDEYRL
                     TGRGTDRVLRVARTVADLRGSEHVGPGDVVSALGFRYVEEREADAA"
     gene            620..1543
                     /gene="dprA"
                     /locus_tag="VEV43_12505"
     CDS             620..1543
                     /gene="dprA"
                     /locus_tag="VEV43_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYP24387.1"
                     /translation="MSRRTIDRSHAEWPSSLRDFTTEEPILRLFVEGMPLPPGARSVA
                     VVGSRRPTSAGVEAAATITRGLVEAGFSIVSGLAVGIDAVAHKTALDHRGHTTAVLGC
                     GHDAAYPRFNAGLRKRIDRCGTVVSEYDLGVPPHKSNFPARNRIIAGISAAVVFVEGG
                     LRSGGLITARHAFDGGRDVFAVPGSFRNPMAAGPNELIRTNQAKLVTDVTHILQDLAP
                     GLVWGEGDADAPPAGDPAVNEGEGRVLLYLDELPTPPDRLCMDLELPWGRAALALAAL
                     EARGYVQKKPGGYAVTTAGARIRSRLPVGDE"
     gene            1708..1869
                     /locus_tag="VEV43_12510"
     CDS             1708..1869
                     /locus_tag="VEV43_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24388.1"
                     /translation="MNEQEKRPDEQDDANDPKTAPVDGPGGDEGEGGLGSTSQGSPGA
                     VEDGGTSGA"
     gene            1912..2817
                     /locus_tag="VEV43_12515"
     CDS             1912..2817
                     /locus_tag="VEV43_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HYP24389.1"
                     /translation="MADVAEVAGGFLRACEAERDLSPHTLAAYRRDLDQFGDWLSRAG
                     ARSIDDVDRLKLRRYVAFLAERKLARRSIARKISAVRSMLAWAVTHGLVESNAAADVP
                     VPTPHRTLPKVMRAGDIARLCELPPGDEPAGQRDRAVLELLYGSGLRVAELCSLDVDD
                     VDVGAARLRVTGKGRKQRQLPMSEPACRAVERYVAGGRKELLRPSTPAEARHALFLNS
                     RGGRLGPRSVRSMLGRYLSAEGAAPVGPHALRHSFATHLLDGGADLRVVQELLGHASL
                     ATSQIYTHVSTERLKAVYERSHPRA"
     gene            2792..3622
                     /gene="whiG"
                     /locus_tag="VEV43_12520"
     CDS             2792..3622
                     /gene="whiG"
                     /locus_tag="VEV43_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HYP24390.1"
                     /translation="MSEAIPEPEATTRAPGPLSSAPEGVQEAWAEYKSTGSGKARDSL
                     ILHYSPLVKYVAGRVAVGLPANIEQADLISYGIFGLIDAIDKYDQSRNIKFETYAISR
                     IRGAIIDELRAIDWVPRSVRFKAREVEKAYSSLENKLKRPPSDKEIADEMGISVDDLH
                     HIYTQLSTVSLVALDELMAVDGGQGDKLSLVDTLEDTKTVSPMDAFESVEMKDILTEA
                     VNKLPEREKVVVTLYYYEGLTLAEIGQVLGVTESRICQMHTKAVLALRGKISEATDPG
                     "
     gene            3737..4759
                     /gene="rpsB"
                     /locus_tag="VEV43_12525"
     CDS             3737..4759
                     /gene="rpsB"
                     /locus_tag="VEV43_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HYP24391.1"
                     /translation="MPVVTMRQLLEAGVHFGHQTRRWNPKMKRFIFAERNGIYILDLQ
                     QTMSGIEKAYTFTRDLVAGGGTVLFVATKRQVQDAVEEQAQRCGMPYVNFRWLGGMLT
                     NFRTMHERIKRMRELEDMDQTGALVTLPKKEALRLRREYEKLARNLGGLRNLDKQPEA
                     VYILDTKKEEIAVREAKKLGIPIVAVLDTNCDPDDVDYAIPGNDDAIRSGALLTRVIA
                     DAVIEGKSMRGDQDEAVTAAAAATVAGDVLPGAAAEPKAEWELQLEAEEQAEREKATQ
                     IATAGGDETPAGGTTTDQTAGTPVEESSLSAPVSGNAADANAPDEKGDEPGDLSHPRP
                     KEDANG"
     gene            4752..5375
                     /gene="tsf"
                     /locus_tag="VEV43_12530"
     CDS             4752..5375
                     /gene="tsf"
                     /locus_tag="VEV43_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HYP24392.1"
                     /translation="MAEISAKDVKALRDATGAGMMDAKKALVETGGDLDKAKRLLREK
                     GLADAAKRTGRAATEGIVYTYMHKPDPNYPPKLGVLLELNCETDFVAKTEAFERLAKD
                     IAMHISFADPQWTTRDQVPQDIIDEESAIYAKQAKDSGKPEQVIEKIVAGKMESFYKE
                     RVLLDQELIQDKSKTIATLIDEARSSMGENVGVGQFARIRVGEGSEG"
     gene            5416..6159
                     /gene="pyrH"
                     /locus_tag="VEV43_12535"
     CDS             5416..6159
                     /gene="pyrH"
                     /locus_tag="VEV43_12535"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYP24393.1"
                     /translation="MEVSPEAKYKRVLVKLSGEAFADPQHGFGIDPRIVASMARQVAE
                     AKELGSELALVIGGGNIVRGLSASAQGTDRTTADYMGMLSTVVNALALQDALEKEGVS
                     TRVQTAIWMQELAEPYIRRRAIRHLEKGRVVIFAGGTGNPYFSTDTAAALRALEIGAE
                     AILKGTRVDGVYDSDPLVNPDARMFSSLPYIEVLNRGLKVMDSTAISLCMDNSLPIIV
                     FNLLAGNIVRILSGEEVGTLVHSDATGGR"
     gene            6206..6727
                     /gene="frr"
                     /locus_tag="VEV43_12540"
     CDS             6206..6727
                     /gene="frr"
                     /locus_tag="VEV43_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HYP24394.1"
                     /translation="MKKAVSVNLEELASIRSGRASPTLLHRINVDYYGSQVPLNQVAN
                     VSVPEPRLMVIAPYDSSQIGAIEKAILASDLGITPNNDGKVIRLAFPQLTEDRRKELI
                     KLAHVRAEEGRVAVRGVRRHAKQELERMKKDHKLSEDEERGAEGQLQKITDKYVAEVD
                     ANLKRKEAELSEV"
     gene            6728..7540
                     /locus_tag="VEV43_12545"
     CDS             6728..7540
                     /locus_tag="VEV43_12545"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYP24395.1"
                     /translation="MSPAAHQRSLKQAVVTALILLGLIVACAIGGRPWFFGLVIAVVL
                     MSVFELLDALVQKGHKPSIVLGLAGTAAILAAAYQEKFAYIGIVLAVATYLAMIYALR
                     PNRGAMAGSDAAWTIFGLTWIAGGGAGAVSILVMEGGVPLLVASVLIVACDDSGAYFV
                     GTSFGRHKLAPSVSPAKSWEGFVGGIGTALAAGLLFAALLDEMTVPSGLAIGLMAGLL
                     APVGDLVESLVKREIGIKDSGRLLPGHGGMLDRLDAIIFCCPAISLYLHFVA"
     gene            7574..>7679
                     /locus_tag="VEV43_12550"
     CDS             7574..>7679
                     /locus_tag="VEV43_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24396.1"
                     /translation="MERRRIVVLGSTGSIGRQALDVAAAHPGLIEVVGV"
BASE COUNT         1326 a         2512 c         2721 g         1120 t
ORIGIN      
        1 tcatggacga gctccccctg tttaggcgag acgtcctaga ggcgctgcgc ggccccctcg
       61 aagatggcgt ggtccaggtc gttcgcagcg gaggctccgt caccttcccg tgcaggttct
      121 ccctcgtcgc agcgatgaac ccatgcccct gcggttacgg cgaggaagga gctctctgtc
      181 cgtgttcgcc gtcggccgtc cgcgcttacc gcgcccgcat ctcggggccg ctgatggatc
      241 ggttcgacct ccacctcacg atgacgcgtc tccgcgctgc cgacttgatg cgcgagaggg
      301 aaggcgagcc gtccgcgacc atccgtgaac gggtcgagca cgcgcgctct atccagcgcg
      361 agaggtaccg agccgccggc gcaaccaacg ccgccgtttc gaaaagaacg ctcctgctcg
      421 cgggccgctt ccgtcccgag gcctacgagc aactgcacgg ggcgatagac gagtaccgcc
      481 ttaccgggcg ggggacggac agggtgcttc gcgtcgctcg gaccgtcgcc gaccttcggg
      541 gaagcgagca cgtcggtccc ggcgacgtcg tctcggccct cggcttccgc tacgtcgaag
      601 agcgagaggc cgatgccgca tgagccgccg aacgatcgac cgaagccatg cggaatggcc
      661 gtcgagcctg cgggacttca cgaccgaaga acccatcctt cgcttgttcg tcgaaggcat
      721 gcccctgccc ccgggtgcgc gcagcgtggc cgtcgtcggc tctcgccggc cgacgagcgc
      781 gggtgtcgag gctgcggcaa cgatcacgag gggactggtg gaagcggggt tctcgatcgt
      841 gtccgggctc gccgtgggga tcgatgcagt cgcgcacaag accgcgctcg atcaccgagg
      901 tcacacgacg gcggtcctgg ggtgcggcca cgacgcggcc tatccgcggt tcaacgcggg
      961 ccttcggaag aggatcgacc gctgcggaac cgtcgtgtcc gaatacgacc tcggcgtccc
     1021 gccgcacaag tcaaacttcc cggcgaggaa tcggatcatt gcggggatca gcgcggcggt
     1081 ggtgttcgtc gagggaggcc ttcgcagcgg cggcctgatc acagcccgtc atgcgttcga
     1141 cggcggacgc gacgtattcg ccgtgcccgg cagcttccga aacccgatgg ccgcgggccc
     1201 gaacgagctc atccgaacga accaggcgaa gttggtgacc gacgtcacgc acatcctcca
     1261 ggacttggcg cccggcttgg tctggggcga aggggacgcg gacgctccgc cggcgggtga
     1321 ccctgcagtg aacgagggcg agggccgcgt cctgctgtat ctcgacgagt tgccgacacc
     1381 cccggacagg ttgtgcatgg atctcgagct gccgtggggt cgtgctgccc tcgctctggc
     1441 cgcgctggag gcccgcggct acgtgcagaa gaaacccggc gggtacgcgg tcacgacggc
     1501 gggcgcgcgc ataaggagcc ggctgcccgt cggagacgaa tgacctttct tccggagctt
     1561 gtggtcgcgg tggttcgtgt ggttggcgag gttgggcttc caaccacgcg caccacacca
     1621 accacgcgca ccagagcccc gggaagagag gtggtgcttc gccgtgggta cagacgcggc
     1681 gtgaagtcgt cgagtgaagg gatcgtcatg aacgaacagg agaaacgacc ggacgagcag
     1741 gacgacgcga acgacccgaa gacggccccc gtggacgggc ccggcggcga cgagggcgag
     1801 ggcggcctcg gctccacctc gcagggctcg cccggcgcgg tcgaggatgg cggcacttcg
     1861 ggggcctagc cggtaattga ctcggccggg cccgggaacc atcctggcgg tgtggctgat
     1921 gttgcggaag ttgccggtgg gttcttgcgg gcttgtgagg ccgagcgcga cctctcgccc
     1981 cacacgctcg ccgcgtacag gcgcgacctc gaccagttcg gcgactggct gagccgggcg
     2041 ggggcccggt cgatcgacga cgtcgaccgg ctgaagctcc ggcggtacgt cgccttcctc
     2101 gccgagcgca agctcgcccg gaggtcgatc gcacggaaga tcagcgccgt caggtcgatg
     2161 ctggcctggg ccgtcacgca cggcctcgtc gagtcgaacg ccgccgccga cgtgcccgtg
     2221 ccgacgccgc accggacgtt gccgaaggtc atgagggccg gggacatcgc ccggctgtgc
     2281 gagctgccgc cgggggacga gccggcgggg cagagggacc gggcggtcct cgagctgctg
     2341 tacgggtcgg ggttgagggt tgcggagctg tgctcgctcg acgtggacga cgtcgacgtg
     2401 ggggccgcgc ggctgcgcgt cacggggaag ggacgcaagc agaggcagct gccgatgagc
     2461 gagccggcct gccgggccgt cgagaggtac gtggccggtg gaaggaaaga gctgctgcgg
     2521 ccgagcacgc ccgcggaggc ccggcatgcc ctgttcctga actcacgggg cgggaggttg
     2581 gggccgcgca gcgtgcgttc tatgcttggt cgatatctgt ccgccgaagg cgccgccccg
     2641 gtgggccccc acgcgctgcg gcattccttc gcgacgcacc tgctggacgg gggggccgat
     2701 ctgcgggtgg tgcaggagct attgggtcat gcgagtctcg ccacgtctca gatctacacg
     2761 catgtctcca cggagcgact gaaagccgtc tatgagcgaa gccatcccag agcctgaggc
     2821 cactacgcga gctcccggcc ccctctcgag tgctcccgag ggggtccagg aggcgtgggc
     2881 cgagtacaag tcgaccgggt cgggcaaggc ccgcgacagc ctgatcctcc attactcgcc
     2941 gctcgtgaag tacgtggcgg ggcgtgtcgc cgtggggctg ccggcgaaca tcgagcaggc
     3001 ggacctgatc tcgtacggga tcttcgggct gatcgacgcg atcgacaagt acgaccagag
     3061 ccggaacatc aagttcgaga cctacgcgat cagccgtatc cgcggggcga tcatcgacga
     3121 gctccgggcg atcgactggg tgcctcgcag cgtgcggttc aaggcccgcg aggtcgagaa
     3181 ggcctactcg agcctcgaga acaagctcaa gcggccgccc tcggacaagg agatcgccga
     3241 cgagatgggg atctcggtcg acgacctcca ccacatctac acgcagctgt cgacggtgtc
     3301 cctcgtagca ctcgacgagc tgatggcagt ggacgggggg cagggcgaca agctgtcgct
     3361 cgtcgacacg ctcgaggaca cgaagacggt ctcgcccatg gacgccttcg agtcggtcga
     3421 gatgaaggac atcctcacgg aggcggtcaa caagctcccc gagcgggaga aggtcgtcgt
     3481 cacgctctac tactacgagg gcctgacgct ggccgagatc ggtcaggtcc tgggggtcac
     3541 cgagtcgcgg atctgccaga tgcacacgaa ggccgtgctg gccctgcggg gcaagatctc
     3601 cgaggcgacc gacccggggt aaactccgcc ggcaccacaa aatccacacg ctcgacatcc
     3661 cggcgcaacg cgggccggga gcttcggtcc cggtaacggg ctcatcgagc ggaggccaac
     3721 cgaaggaggt ccttctatgc ccgtcgtcac gatgcggcag ctactggagg ctggggtcca
     3781 tttcggacac cagacccggc gatggaaccc gaagatgaag cggttcatct tcgcggagcg
     3841 caacggcatc tacatcctcg acctgcagca gacgatgtcg gggatcgaga aggcctacac
     3901 gttcacccgc gacctcgtcg ccgggggcgg gacggtcctc ttcgtcgcca cgaagcgcca
     3961 ggtccaggac gccgtcgagg agcaggccca gcgctgcggg atgccgtacg tgaacttccg
     4021 gtggctcggc gggatgctca cgaacttccg gacgatgcac gagcgcatca agcgcatgcg
     4081 cgagctcgag gacatggacc agaccggcgc gctcgtcacg ctgccgaaga aggaagcgct
     4141 gcggctccgg cgcgagtacg agaagctcgc ccgcaacctc gggggcctgc gaaacctcga
     4201 caagcagccc gaggcggtct acatcctcga cacgaagaag gaagagatcg ccgtgcgcga
     4261 ggcgaagaag ctcggcatcc cgatcgtcgc ggtgctcgac acgaactgcg accccgacga
     4321 cgtcgactac gcgatccccg gcaacgacga cgcgatccgt tcgggcgcgc tcctcacgcg
     4381 cgtcatcgcc gacgccgtga tcgagggcaa gtcgatgcgc ggcgaccagg acgaggccgt
     4441 gacggctgcc gccgcggcga ccgtcgcagg tgacgtgctt ccgggggccg cggccgagcc
     4501 gaaggccgag tgggagctcc agctcgaggc cgaggagcag gccgagcgcg agaaggcgac
     4561 ccagatcgcc acggcaggcg gcgacgagac gcccgcgggc ggcacgacga ccgaccagac
     4621 cgccgggacc cccgtggaag agagctcgct gtcggccccc gtctccggaa acgcggcgga
     4681 cgccaacgcc ccggacgaga agggcgacga gccgggagac ctctcacacc ccaggccgaa
     4741 ggaggacgca aatggctgag atatccgcga aggacgtgaa ggcgctacgc gacgcgaccg
     4801 gcgccgggat gatggacgcg aagaaggcgc tcgtcgagac cggcggcgac ctcgacaagg
     4861 cgaagcgtct gctgcgcgag aagggccttg ccgacgcggc gaagcgcacc ggccgtgcgg
     4921 cgaccgaggg catcgtctac acctatatgc acaagcccga cccgaactac ccgccgaagc
     4981 tcggcgtgct gctcgagctc aactgtgaga cggacttcgt cgcgaagacc gaggccttcg
     5041 agcggctcgc gaaggacatc gcgatgcaca tctcgttcgc cgacccgcag tggacgaccc
     5101 gcgaccaggt cccgcaggac atcatcgacg aggagtcggc gatctacgcg aagcaggcga
     5161 aggactccgg caagcccgag caggtgatcg agaagatcgt cgccggcaag atggagtcgt
     5221 tctacaagga gcgcgtcctt ctcgaccagg agttgatcca ggacaagtcg aagacgatcg
     5281 ctacgctgat cgacgaggcc cgctcgagca tgggcgagaa cgtcggcgtc gggcagttcg
     5341 cccgcatccg cgtcggcgag ggctcggagg gctagcgacg ttgaaccggt cggctcggcg
     5401 agcggtccag gcgtgatgga agtctcgccg gaagcgaagt acaagcgagt cctcgtaaag
     5461 ctctccggcg aggccttcgc cgacccccag cacgggttcg gcatcgaccc gcggatcgtc
     5521 gcgtcgatgg cgcggcaagt cgcagaggcg aaggagctcg gctccgagct cgcgctcgtg
     5581 atcggcggcg gcaacatcgt gcgcggcctc tccgcgtcgg cccaggggac cgaccgcacg
     5641 accgcggact acatgggcat gctctcgacg gtcgtcaacg cgctcgcgtt gcaggacgcg
     5701 ctcgagaagg agggcgtcag cacccgcgtc cagactgcga tctggatgca ggagctcgcc
     5761 gagccgtaca tccgcaggcg cgcgatccgg cacctcgaga agggccgcgt cgtgatcttc
     5821 gccggtggga ccggcaaccc gtacttctcg acggacaccg cggccgcgct ccgggcgctc
     5881 gagatcgggg ccgaggcgat cctcaagggg acgcgggtcg acggcgtcta cgactcggac
     5941 cctctcgtga acccggacgc gcgcatgttc tcgtcgctgc cgtacatcga ggtcctgaac
     6001 cgcgggctga aggtcatgga ctcgacggcg atctcgctgt gcatggacaa ctcgctgccg
     6061 atcatcgtgt tcaacctcct cgccgggaac atcgtgcgga tcctctcggg tgaggaagtg
     6121 ggaaccctgg ttcactccga cgcgacagga ggacgttgac gtgttcgaga ccaaggacga
     6181 ggtcctgagg gacgcggacc agaagatgaa gaaggcagtg tcggtgaact tggaggagct
     6241 ggcctcgatc cgctcgggcc gcgcttcgcc gacgctgctg caccggatca acgtcgacta
     6301 ctacggctcg caggtcccgc tcaaccaggt ggccaacgtg tcggtgcccg agcctcgcct
     6361 gatggtgatc gctccgtacg actcgagcca gatcggcgcg atcgagaagg cgatcctcgc
     6421 gtccgacctc ggcatcacgc cgaacaacga cgggaaggtc atccggctcg cgttcccgca
     6481 gctgaccgag gaccggcgca aggagctgat caagctcgct catgtgcgcg ccgaagaggg
     6541 ccgcgtcgcc gtccgcggcg tgcgccggca cgcgaagcag gagctcgagc ggatgaagaa
     6601 ggaccacaag ctctccgagg acgaagagcg cggcgccgag ggccagctgc agaagatcac
     6661 cgacaagtac gtggccgagg tcgacgcgaa cctgaagcgc aaggaagcag agctctccga
     6721 ggtctgattg tcaccggccg cccatcagcg atcgctgaag caggcggtcg taacagccct
     6781 catcctgctc ggcctcatcg tggcctgcgc catcggcgga cggccgtggt tcttcgggct
     6841 cgtgatcgcg gtcgtgctga tgtcggtctt cgagctgctc gacgccctcg tccagaaggg
     6901 ccacaagccg agcatcgtcc tcggcctcgc ggggacggcg gcgatcctcg ccgcggcgta
     6961 ccaggagaag ttcgcctaca tcgggatcgt cctcgcggtg gcgacgtacc tcgcgatgat
     7021 ctacgcgctg cggccgaacc gtggcgcgat ggcggggtcg gacgcggcgt ggacgatctt
     7081 cggcctcacc tggatcgcgg gcgggggggc cggcgcggtg tcgatcctcg tcatggaggg
     7141 aggcgtcccg cttctcgtcg cctcggtcct gatcgtggcg tgcgacgaca gcggcgcgta
     7201 cttcgtgggc accagcttcg gccggcacaa gttggcgccg tccgtctcgc cggcgaagtc
     7261 gtgggagggg ttcgtgggcg gcatcggaac cgcacttgcg gcagggctcc tgttcgccgc
     7321 cctcctcgac gagatgaccg ttccgtcggg gctggcgatc ggcctcatgg ccgggctgct
     7381 cgcccccgtc ggcgacctcg tcgagtcgct ggtgaagcgc gagatcggga tcaaggactc
     7441 gggacggctg ctgccgggcc acggcgggat gctcgatcgc ctcgacgcga tcatcttctg
     7501 ctgccccgcg atctcgctat atctccactt cgtcgcctga ggtctatgac aaaggccgtg
     7561 tgagaatccc cgcgtggaga gaagacgcat cgtcgtgctc ggctcgaccg gttcgatcgg
     7621 gcgacaggcc ctcgacgtcg ccgccgcaca cccgggcctg atcgaggtcg tcggggtcg
//
LOCUS       DASQDO010000459         4529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_171476, whole genome shotgun sequence.
ACCESSION   DASQDO010000459 DASQDO010000000
VERSION     DASQDO010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171476"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VEV43_12555"
     CDS             <1..225
                     /locus_tag="VEV43_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24397.1"
                     /translation="MLKDWARRTKADVTVELTGPAGGLYVAGDGRRATITASAGAVVH
                     RLAGREPSGTITLGGDVAAAERWLAAGCPV"
     gene            complement(222..1076)
                     /locus_tag="VEV43_12560"
     CDS             complement(222..1076)
                     /locus_tag="VEV43_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24398.1"
                     /translation="MDRRFARRAWQHLETLHAVVYFAPEKKAAYDAAGLKGGWMGYFA
                     GRAAPLGPVSAEVVTAVFYNFHPAMVRRAIPDAWAFSSPERVLEARLEIVDAALRRLL
                     GADLGALGDAAELCLDAASRCDAAGRPLLAANAALPAPDEPHLRLWHAATMLREHRGD
                     GHVAALVTAGLDGCEAHLTLAGTGALAEADLRPHRGWPDEDWEAARARLVERGLLDES
                     GTLTRSGAELRAWIEDRTDDAALGPWRSLGDGASERLLMLLERPVERVLAGGGVPFPN
                     PMGLPRPG"
     gene            1197..1427
                     /locus_tag="VEV43_12565"
     CDS             1197..1427
                     /locus_tag="VEV43_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP24399.1"
                     /translation="MQTTLPAPTDEERAVASLLVTGMTDEMAARRLGLSRRTFRRRLK
                     ALMDKLGARSRFQAGFMLAETGWATEQTLERD"
     gene            complement(1459..1947)
                     /locus_tag="VEV43_12570"
     CDS             complement(1459..1947)
                     /locus_tag="VEV43_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24400.1"
                     /translation="MLKKTLVTLVAAALVAAPVGTGAFAKGKKKKGPKPYVSEEITIQ
                     SGHPAAHSQSGTLVTITAQEFIRTCALPSTNGVDAAVYEVPADYKGLTASIKALGTGA
                     GPAGYDLDLYLYDESCTEVAVYNPSNTDEFGVLTPDTAFILVHNYQPGPITQHIELAP
                     SK"
     gene            2082..4505
                     /locus_tag="VEV43_12575"
     CDS             2082..4505
                     /locus_tag="VEV43_12575"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HYP24401.1"
                     /translation="MARTRRRCAALTTLLVLASFAPAGAAPAQGRVAARSVTRGAWSE
                     PATWGGSPPGRGDVVVIPAGSHVVLDTSTPQLGGLQVDGTLSFAPKDLELTTDWIVVH
                     GRMRVGTAKRPFRGNAVITLTGVPSEEDVMGMGTKLLGVMGGTLDLHGRRVRTWARLD
                     ATAGRGADRITLDGDVPWGPGDRVALASSDYWRQHDEVRTITAVDGRTLRLDSPLDYR
                     HWGEVQTFDGHPVDERAEVALLTHNVVVRGDETSNEDGFGGHLMVMEGSTARIEGVEL
                     ARMGQRKALRRYPVHFHMDGDADGSYLKRSTIHDSFNRCVVVHGTHDLVVRDNVCFDH
                     AGHGFFLEDGIETGNLIAGNLGFGTRKVEDGLLPSDERPATFWITNPDNVLRGNVAGG
                     SDGFGFWYALPRHPTGLSEDEGVWPRRTPLGEFSRNVAHSNAQSGLNVDDGPRPDGRT
                     ESTSYEPVADPSDPDSEPVVARFEHLVAYMNRSRGVWLRGEHHVVTGATLADNRAGAT
                     FASSESYLEDSVVVAETHNPGMTEPWEETGPNGRALPAFWDPEAPIGGFEFYDGKVGV
                     RDTTFVGFRSNSLRQAGGLTYLAPNAFSLDPRNFAERVRFVDSNPVYLTEPEPGMDGD
                     ASKVFYDRDGTVTGRAGSAVVVDNPFLLNDTCEYRAAWNAHVCDSDYVSLLAGSESGG
                     RSWIDPLTIRRPDGVEQKLAGCCDYSDEAYTTVVPGRPYEVEFAGGVPDEVKLVLFNG
                     RGRWIRVSLRVSGPVRVTRWGRALQQVAGLDALGSRSDSGWHFDAATSTVHVKLYGHG
                     DWEEVRIQS"
BASE COUNT          650 a         1620 c         1614 g          645 t
ORIGIN      
        1 atgctgaagg actgggcgcg gcggacgaag gcggacgtga cggtcgagct cacgggcccc
       61 gcgggcggcc tctacgtcgc cggcgacggg aggcgcgcga cgatcaccgc gtccgccggc
      121 gcggtcgtgc accgcctcgc cgggcgcgag ccctccggga cgatcacgct cggaggcgac
      181 gtcgcggcgg ccgagcgctg gctcgccgcg ggctgccccg tctagccggg ccgcggcaag
      241 cccatcgggt tcgggaacgg gacgccccca cccgcgagga ccctctcgac cggcctctcc
      301 aacagcatga gcagcctctc gctcgcgccg tcacccaacg acctccacgg ccccagggcg
      361 gcgtcgtcgg ttcggtcctc gatccacgcc cgcagctcgg cccccgagcg cgtgagcgtg
      421 ccggactcgt ccagcaaccc gcgctcgacg agccgcgccc gcgcggcctc ccagtcctcg
      481 tccggccatc cccggtgggg ccgcaggtcg gcctccgcga gggcgcccgt cccggccagc
      541 gtgaggtgcg cctcgcaccc gtcgagcccg gcggtcacga gagcggctac gtggccgtcc
      601 ccgcggtgct ctcgcagcat cgtcgccgcg tgccagagcc tgaggtgcgg ctcgtcgggg
      661 gccggcagcg cggcgttcgc cgccaacagc gggcgccccg cggcgtcgca gcgggaggcg
      721 gcgtcgaggc acagctcggc cgcgtcgccg agggcgccga ggtcggcgcc gagcaatcgg
      781 cgcagcgccg cgtcgacgat ctcgaggcgc gcttccagca ctcgctccgg cgacgagaac
      841 gcccatgcgt cggggatggc gcgccgcacc atcgcggggt ggaagttgta gaagacggcg
      901 gtgacgacct ccgcggagac cgggcccaga ggcgcggccc ggccggcgaa gtagcccatc
      961 cacccgccct tcaaccccgc tgcgtcgtaa gcggccttct tctcgggagc gaagtacacg
     1021 accgcgtgca gggtctcgag gtgctgccac gcgcggcgcg caaaccgtcg gtccacccaa
     1081 cccttatacc gccgcgtgaa ggtgctcccc catccggggg atcacgggcg tcgtcgaaat
     1141 cgccgcgtta cgcgcccggc agactttcgt tctacgcagc gatgacgacg aacgagatgc
     1201 agaccacatt gccggcgcct accgacgagg agcgcgcggt cgcctcactg ctcgtgacgg
     1261 ggatgacgga cgagatggcg gcacgccgtc tcggcctcag ccgtcgcacg ttccgccggc
     1321 gtctcaaggc gctgatggac aagttgggcg cgcgctcgcg cttccaggcg gggttcatgc
     1381 tcgccgagac cggttgggcg acggagcaaa ccctagagcg cgactagcac cggcagcctg
     1441 aaccctgcgt tcgcgtcctt acttcgaggg cgcgagctcg atgtgctgcg tgatggggcc
     1501 gggctggtag ttgtgcacga ggatgaacgc cgtgtcgggt gtgagcaccc cgaactcgtc
     1561 ggtgttcgac gggttgtaca ccgcgacctc ggtgcagctc tcgtcgtaca ggtagaggtc
     1621 gaggtcgtag ccggcgggac cggcccccgt gccgagcgcc ttgatcgacg cggtgagccc
     1681 cttgtagtcc gccggcacct cgtagacggc tgcgtcgaca ccgttggtcg acgggagggc
     1741 gcacgtgcgg atgaactcct gcgcggtgat cgtcacgagc gtccccgact ggctgtgcgc
     1801 ggcggggtgg ccgctctgga tcgtgatctc ttcgctcacg tacggcttcg ggcccttctt
     1861 cttcttgccc ttcgcgaagg caccggtgcc cacgggggcc gcgacgagcg cggcggccac
     1921 gagcgtgacg agggtcttct tgagcatcgt tttcgtccct tcgttgcaga ggtctcggaa
     1981 cccctattcg gcgcctccgg caccccctcc tgccggacgg aacccacgcg atccggacat
     2041 ctccttacag cgccccgccg cgatgccgat aaccgggggg aatggcccgt accaggcgcc
     2101 gctgcgccgc cctcacgacc ctgctcgtcc ttgcctcgtt cgcccctgcc ggggccgctc
     2161 ccgcgcaagg acgggtcgcc gcgcgctccg ttacgcgagg ggcgtggtcc gagcctgcga
     2221 cctggggcgg atcgcctccg gggcgcggtg acgtggtcgt gatcccggcg ggctcgcacg
     2281 tggtgctcga cacctcgacg ccgcagctcg gcggcctcca ggtcgacggc acgctctcgt
     2341 tcgcgccgaa ggacctcgag ctgacgaccg actggatcgt ggtgcacgga cgtatgcgcg
     2401 tcggcaccgc caagagaccg ttccgcggga acgccgtgat cacgctcacc ggcgtgccta
     2461 gcgaagaaga cgtgatgggc atgggcacga agctgctcgg cgtcatgggc ggaacgctcg
     2521 acctccacgg caggcgcgta cgtacgtggg cacgtctcga cgcgaccgcg ggccgcggcg
     2581 ccgatcggat cacgctcgac ggtgacgtgc cgtgggggcc gggcgaccgg gtcgcgcttg
     2641 cgtcgtcgga ctactggcgt cagcacgacg aggtccgcac gatcacggcc gtcgacgggc
     2701 gcacgctgcg gctcgactcg ccgctggact accgccactg gggcgaggtg cagacgttcg
     2761 acggccatcc cgtcgacgag cgcgccgagg tcgcgctgct cacccacaac gtggtcgtgc
     2821 gcggagacga gacgtccaac gaggacggtt tcggcgggca cttgatggtg atggagggct
     2881 cgaccgcccg catcgagggc gtcgagctcg cgcgcatggg ccagcgcaag gcgctgcggc
     2941 gctacccggt gcacttccac atggacggcg acgccgacgg ctcgtacctg aagcgctcga
     3001 cgatccacga cagcttcaac cgctgcgtcg tcgtgcacgg cacgcacgac ctcgtcgtgc
     3061 gcgacaacgt gtgcttcgac cacgcgggac acggcttctt cctcgaagac gggatcgaga
     3121 ccggcaacct gatcgccggc aacctcggct tcggcacgcg caaggtcgag gacggcctcc
     3181 tgccgagcga cgaacggccc gccacgttct ggatcacgaa ccctgacaac gtcctgcggg
     3241 gcaacgtcgc ggggggctcg gacggcttcg gcttctggta cgcgctgccg cgccatccga
     3301 cgggcttgtc cgaggacgaa ggggtctggc cgcgccggac gcccctcggt gagttcagcc
     3361 ggaacgtcgc gcactcgaac gcgcagtcgg gcctcaacgt cgacgacggc ccccgccccg
     3421 acggccggac cgagtccacg tcctacgagc ccgtcgccga cccctccgac ccggactccg
     3481 agccggtggt ggcccgcttc gagcacctcg tcgcgtacat gaaccgcagc cgcggagtgt
     3541 ggctgcgagg ggagcaccac gtcgtcaccg gcgcgacgct cgccgacaac cgcgccggag
     3601 caacgttcgc gtcgtcggag agctacctcg aagacagcgt cgtcgtcgcg gagacgcaca
     3661 acccggggat gaccgagccg tgggaggaga ccgggccgaa cggccgcgct cttcctgcgt
     3721 tctgggatcc cgaggcgccg atcggaggct tcgagttcta cgacgggaag gtcggggtgc
     3781 gcgacacgac cttcgtaggg ttcaggtcca actcgctgcg tcaggcgggc gggctcacgt
     3841 acctggcgcc gaacgcgttc tcgctcgacc cgcgcaactt cgccgagcgc gtgcgcttcg
     3901 tcgactcgaa ccccgtctac ctgacggagc cggagcccgg catggacggc gacgcctcca
     3961 aggtcttcta cgaccgcgac gggaccgtca ccggccgcgc ggggagcgcg gtcgtcgtgg
     4021 acaacccctt cctgctgaac gacacgtgcg agtaccgcgc cgcgtggaac gcgcacgtgt
     4081 gcgactcgga ctacgtctcg ctgctcgcgg ggagcgagag cgggggccgg agctggatcg
     4141 acccgctcac gatccggcgt cccgacgggg tcgagcagaa gctcgccggc tgctgcgact
     4201 actccgacga ggcgtacacg acggtcgtcc ccggccggcc gtacgaagtc gagttcgccg
     4261 gcggggtgcc ggacgaggtg aagctcgtgc tgttcaacgg gcgggggcgt tggatccgcg
     4321 tgtcgctgcg ggtgtccggc ccggtccgcg tgacccgctg gggccgcgcc ctacagcagg
     4381 tcgccgggct cgacgctctc gggagccgtt ccgactcggg ctggcacttc gacgccgcga
     4441 cgtcgacagt gcacgtgaag ctctacgggc acggcgactg ggaagaggtc cggatccagt
     4501 cctgagtcag gcccacgggc cgatgccgg
//
LOCUS       DASQDO010000460         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1027802, whole genome shotgun sequence.
ACCESSION   DASQDO010000460 DASQDO010000000
VERSION     DASQDO010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027802"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VEV43_12580"
     CDS             complement(<1..1111)
                     /locus_tag="VEV43_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYP24402.1"
                     /translation="MKLRLAAALLAVLAACGGNAGYDVLPDRSMAAARGITLPEPAPA
                     IPAFLLRFENAILPTRAEKAANMRGVSVAVPLTLTERRVEGPTGISTLRVGAVDPLLF
                     RALAPEPTRAAEFVWSALVSGEAVVTFEAAEALGIEGSGEITVGDTPLRAGAIADNGV
                     PNLADVLVPDFVGEDAGLPGPRLLAIGVKDADRIAATGRALREGFPGAQVRRLLGEVP
                     EPIEPSSPELVGSSSGGLIGTLHFEVAEDGFIRPDPAWVAANIAHADMPIIGSVTCHR
                     LLIPQLHAALAEVEEAGLAGEIYEYNGCYVPRFIDRDPSKPLSMHAFGLAIDLNTATN
                     QLGTEGDMDPRVVEIFERWGFVWGGRWSRPDPMHFE"
     gene            complement(1108..1836)
                     /locus_tag="VEV43_12585"
     CDS             complement(1108..1836)
                     /locus_tag="VEV43_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HYP24403.1"
                     /translation="MAAAGFLPKTAALVRKDLRIEARGRDTLPPMLAFTLAVVLLLAF
                     SLPDASRLDQAVRIPTGGTLLLADVLAGFLWVTVLFAGLIGFARTFEVEREGGALDVL
                     LLVPLDRAGLFAAKAVANLVFVLSVELLLLPVYALLFGIDLGSGWFALVVVVLLVDVG
                     FVCVGTLFSALAAQTSSRELMLPLLALPALGPVFVAATELTSDLFTGAGLDAVAARGW
                     FTILAVFDVVFATAGALVFDFTLE"
     gene            complement(1836..2543)
                     /gene="ccmA"
                     /locus_tag="VEV43_12590"
     CDS             complement(1836..2543)
                     /gene="ccmA"
                     /locus_tag="VEV43_12590"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HYP24404.1"
                     /translation="MTEAMVRLHGVEVAFGRTLALHGMTIDLFPGVIGLFGPNGSGKS
                     TLLSVLAGLLRPTAGTAEYRGRPIRSSDEEWRRVVGYAGHEPGLYPSLTVAENLELWA
                     QLFDAPGRRVGEMIERLGLGERRNTQVRALSAGYKRRASVARALLHEPAVLLLDEPYA
                     NLDDEAAAEVSAAIVEWRRPDRLAVVATHGAKKVKAYADAGIILKHGAVVSYRRGVEQ
                     LAPPVASASGRSGSAGT"
     gene            complement(2540..3691)
                     /locus_tag="VEV43_12595"
     CDS             complement(2540..3691)
                     /locus_tag="VEV43_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYP24405.1"
                     /translation="MPDHHVRAALALTLVLLTAAPVEAAETGTIRGRVLNETTGEPQE
                     GVSVTLTSALDDGSRQRAVVTTTDERGRYLFEDLPTGDDRYYAIDARHDDGLFAGGVV
                     RLPEGTAKPPVIDSTLRVWDTTTDPAAIVVRRDSIFVVPSDDGAGVIEALVVANISEE
                     AYIGRGRELGAEQDGEIASLGFAVPREARLPPVPIVESEIDFPTLVETSFGFAATTAI
                     PPGQWRVVYSYEVPGDGGTYDLSRPALYPTLELGVYAGEPLRIEGGRVSPDGSRDVEG
                     KSYDLYSSSGTLEAGDSIDMIAVAEASAGNLLAIGGIVAAVVVAAGALVFVIRRRSRG
                     TAPEPPAREDVVEAIARLDLAKEQGLIQETEWASRRDALKRSLEEERVP"
     gene            complement(3673..>4016)
                     /locus_tag="VEV43_12600"
     CDS             complement(3673..>4016)
                     /locus_tag="VEV43_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24406.1"
                     /translation="LPLGKRGRSEKEPSAEGEVEARKTTALSAIVDLENERLVGKLSD
                     EDFDVLRVQYESEALQALTELDALRAESDEPDDASLEAEIAEMRQRLACPRCGSLRST
                     DGTCAQCQTTT"
BASE COUNT          594 a         1434 c         1448 g          540 t
ORIGIN      
        1 gctcgaagtg catcgggtcg ggccgggacc agcgcccgcc ccacacgaac ccccaccgct
       61 cgaagatctc gaccacgcgg gggtccatgt cgccctcggt gccgagctgg ttcgtcgccg
      121 tgttgaggtc gatcgccagg ccgaacgcgt gcatcgacag cggcttgctc gggtcccggt
      181 cgatgaagcg ggggacgtag cacccgttgt actcgtagat ctcgccggcg agcccggcct
      241 cctcgacctc ggcgagcgcg gcgtggagct gggggatcag caggcggtgg cacgtgaccg
      301 agccgatgat cggcatgtcg gcgtgcgcga tgttcgcggc cacccatgcc gggtcgggcc
      361 ggatgaaccc gtcctccgct acctcgaagt ggagcgtgcc gatgaggccg ccggagctcg
      421 agcccacgag ctcgggggag ctcggctcga tgggttcggg gacctcgccg aggagccggc
      481 ggacctgcgc gccggggaac ccctctcgga gggcgcgccc cgtggcggcg atccgatccg
      541 cgtccttcac gccgatcgcg aggagacggg ggccggggag cccggcgtcc tcgccgacga
      601 agtcgggcac gaggacgtcc gcgaggttcg gcacgccgtt gtccgcgatc gcgccggcgc
      661 gcagaggcgt gtcgccgacg gtgatctctc cggagccctc gatgcctagc gcttcggcgg
      721 cctcgaacgt cacgacggct tcgccggaga cgagcgccga ccagacgaac tcggcggcgc
      781 gcgtgggctc gggcgcgaga gcgcggaaca gcagcgggtc gactgcgccc acgcgcaaag
      841 tcgagatccc cgtcgggccc tcgacacgcc gttcggtgag cgtgagcggc accgccaccg
      901 agacgccccg catgttcgcc gccttctcgg cacgcgtcgg caggatcgcg ttctcgaacc
      961 tgaggaggaa ggcggggatc gcgggggccg gctcgggcag cgtgatcccg cgcgccgcgg
     1021 ccatcgaccg gtcgggcagg acgtcgtagc ccgcgttgcc gccgcacgcg gccagcacgg
     1081 cgaggagcgc ggcggcgagc cggagcttca ttcgagcgtg aagtcgaaga cgagggcgcc
     1141 ggcggtcgcg aacacgacgt cgaacaccgc gaggatcgtg aaccagccgc gcgccgcgac
     1201 cgcgtccagc ccggcccccg tgaagaggtc cgaggtgagc tccgtagccg cgacgaagac
     1261 ggggccgagc gcggggagcg cgagcagggg cagcatcagc tcgcgcgacg acgtctgggc
     1321 ggcgagcgcc gagaagagcg tccccacgca tacgaacccg acgtcgacga gcagcacgac
     1381 gacgacgagc gcgaaccagc cggagccgag gtcgatcccg aacaacagcg cgtagacggg
     1441 gagcaggagc agctcgaccg acaggacgaa gaccaggttc gctacggcct tcgcggcgaa
     1501 cagcccggcc cggtcgagcg ggaccagcag cagcacgtcg agcgcgccgc cctcgcgctc
     1561 gacctcgaac gtgcgcgcga acccgatcag cccggcgaac aggaccgtga cccacaggaa
     1621 accggccagc acgtcggcga gcaggagcgt gccgccggtg gggatgcgga ccgcctggtc
     1681 gaggcgcgac gcgtccggta gcgagaacgc gaggagcaat acgacggcga gcgtgaacgc
     1741 gagcatcggg ggcaacgtgt cgcggccccg ggcttcgatc cgcaggtcct tgcggacgag
     1801 cgcggcggtc ttcgggagaa agcccgcggc cgccatcagg tgcctgcact tccgcttcga
     1861 ccactcgccg aggcgaccgg tggcgcgagc tgctcgacac cgcgccggta gctcacgacg
     1921 gccccgtgct tgaggatgat ccccgcgtcc gcgtaggcct tgaccttctt cgcgccgtgc
     1981 gtcgcgacga ccgcgagacg atccggccgg cgccactcga cgatcgcggc ggacacctcg
     2041 gccgcggcct cgtcgtcgag gttcgcgtag ggctcgtcca gcaagagcac ggcgggctcg
     2101 tggagcaggg cccgcgcgac ggacgcgcgc cgcttgtatc cggccgagag ggcacggacc
     2161 tgcgtgttcc ggcgctcgcc gaggccgagt ctttcgatca tctctcccac tcgacgtccc
     2221 ggcgcgtcga acagttgcgc ccacagctcg aggttctcgg cgacggtcag cgacggatag
     2281 aggcccggct cgtgccccgc gtatccgacg acgcgccgcc actcctcgtc ggaggagcgg
     2341 atcgggcggc cgcggtactc ggccgttccg gcggtcgggc ggaggaggcc cgccagcacc
     2401 gacagcaacg tcgacttccc cgagccgttc ggaccgaaca aaccgatcac gccgggaaac
     2461 aggtcgatcg tcatcccgtg gagggcgagc gtgcggccga acgcgacctc gacgccgtgg
     2521 agccggacca tcgcctcggt cacggcaccc gctcctcttc cagggaccgc ttcaacgcgt
     2581 cccgccgcga cgcccactcg gtctcctgga tgaggccctg ctccttggcg aggtcgagac
     2641 gcgcgattgc ctcgacgacg tcttctctcg cggggggctc gggtgcggtc ccgcgcgacc
     2701 ggcggcggat gacgaacacg agcgcgcccg cggcgaccac aacggccgcg acgatgcctc
     2761 cgatcgcgag gaggttcccc gccgacgcct cggcgaccgc gatcatgtcg atcgagtcgc
     2821 cggcttcgag cgtgccgctg ctcgagtaga ggtcgtacga cttgccctcg acgtcgcggc
     2881 tcccgtcggg gctcacgcgg cccccttcga tcctcagcgg ctcgcccgcg tacacgccga
     2941 gctcgagcgt cgggtagagc gcggggcgcg agaggtcgta cgtcccgccg tcgccgggca
     3001 cctcgtatga gtagacgacg cgccactgtc ccggtgggat cgcggtcgtc gcggcgaagc
     3061 cgaagctcgt ctcgaccagc gtcgggaagt cgatctcgga ctcgacgatc gggacgggtg
     3121 ggagccgagc ctcgcgcggg acggcgaacc cgaggctcgc gatctcgcca tcctgttccg
     3181 cacccagctc acggccacgc ccgatgtaag cctcctcgga gatgttcgcc acgacgaggg
     3241 cttcgatcac gcccgcgccg tcgtcgctcg gcacgacgaa gatgctgtcg cggcgcacga
     3301 cgatcgcggc ggggtcggtc gtcgtgtccc acacccgcag ggtcgagtcg atcaccgggg
     3361 gcttcgccgt gccctcggga agtcgcacga ctccgccggc gaagaggccg tcgtcgtgcc
     3421 gggcgtcgat cgcgtagtag cgatcgtccc cggtggggag gtcctcgaag agatatctcc
     3481 cgcgctcgtc ggtcgtcgtg acgaccgcgc gttgccgcga cccgtcgtcg agcgcgctcg
     3541 tcagggtcac gctgacgccc tcttgcggct cgcccgtcgt ctcgttcagc acgcgtcccc
     3601 ggatcgtgcc ggtctcggct gcttccacgg gagcggccgt cagcagcacg agcgtgagcg
     3661 cgagcgcggc cctcacgtgg tggtctggca ttgcgcgcac gtcccgtcgg tagaccgcag
     3721 gcttccgcag cgcgggcacg cgaggcgctg ccgcatctcc gctatctcgg cctcgaggga
     3781 cgcgtcgtcc ggctcgtcgc tctcggcgcg cagcgcgtcg agctccgtca gcgcttgcaa
     3841 cgcctcggac tcgtactgca cgcgcagcac gtcgaagtcc tcgtcggaga gcttgccgac
     3901 gagacgctcg ttctcgaggt cgacgatcgc cgagagagcc gtcgtcttgc gtgcctcgac
     3961 ctcgccttcg gcggacggct ccttctcgga ccgtccccgc ttgccgagcg ggagcg
//
LOCUS       DASQDO010000461         9052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_966943, whole genome shotgun sequence.
ACCESSION   DASQDO010000461 DASQDO010000000
VERSION     DASQDO010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966943"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VEV43_12605"
     CDS             complement(<1..153)
                     /locus_tag="VEV43_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HYP24407.1"
                     /translation="MTERLVVVGGDAGGMTAASQARRLRGPDDLDIVAFERGHFTSYS
                     SCGIPYF"
     gene            286..573
                     /locus_tag="VEV43_12610"
     CDS             286..573
                     /locus_tag="VEV43_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24408.1"
                     /translation="MQVEDKVCHELKEGLTLALGWLETLFQRWQTLPDAQRQQMVAGA
                     LLGVNRATFALDQIEGKSVEDIASPEERMASELVKLVEDPIEIAAGNGALR"
     gene            721..1788
                     /locus_tag="VEV43_12615"
     CDS             721..1788
                     /locus_tag="VEV43_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP24409.1"
                     /translation="MRVFPKRRLVALSALVATALSLGLTGAGAQTTTPNDPLFANQWG
                     LQKIQAPAAWSLADGTGITIAIVDSGVDLGHDDFACGKLAVHPGSNIGSRKSPNTPQD
                     ADGHGTHVAGIAGACTNNGVGVAGVAPGSTIMPVSVFNESDLDKAMADGIRFATDRGA
                     HVINLSIGDIPPFSHGGALLFPQTEDALEYARNSGVVIAAAAGNFDQPTCEYPSFSRN
                     VICVVATDSNDVRSYYSDFPVNGDPQTLPAMEPVVAAPGGQGTFCDEGIVSTYLRSLD
                     SVCYSTGYESLDGTSMSSPHVAGVAALVYQRLGGVRSAANADLVVQTILDSADDLYTP
                     GWDPIVGHGRVNALTAVQSVP"
     gene            1942..3987
                     /locus_tag="VEV43_12620"
     CDS             1942..3987
                     /locus_tag="VEV43_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP24410.1"
                     /translation="MPRRFHTISLVALASLVCAVLAAIPVRAGAGEGPAPVDRPVLRA
                     TRRTSALVHVVEGTALRDGIAAARAAGLETGTVYDAIRVFVAYGSATEFSELAKAPSV
                     GYVEANRRMRLFSDTSHGATRGQDVLDGAVTLAGGATIDGTGVGVAVVDTGVDGTHPD
                     LAPRMGGNVKMVCTVPLIVNGPEDGFTECRGPKTAVELEDTDHASPSGHGTHVAGIVA
                     GTGEASAGEYHGAAPGVTLYGVGMGTVVLPENALDGLAWVLANHDQVSPPIKVVNTSW
                     GTSYSKYRPSGDPTYSATWKLQEELVEAGITIVVSAGNGAGTGTAATTSAQCVNPTPG
                     IVCVANYDDQGTGTRAGTIDPGSSRGSTSDVESWPDVSAPGTLITSTCRMTLPICHLH
                     DGMLLDPPNRYATLTGTSMAAPHVAGIAAQLYQADPSITPAEIENVLEDTAHKFAFGA
                     AYGLLTDATNPDDTSSYEKGHGLVDALAAVEAVLNAGTTPTPSPSPTATPTAPPPSGT
                     RYYFQTATWVGEADGVSSGSTFDSETPAALIYAEWRDLPRSNERPADPFDPHWTGRVD
                     GPIEGLTLDFWAKTPVGEELGTVSYRPVVWVGDDPYVLPTLTVPMQPSLGNVPTRLTR
                     SYTTRLDAAGNEVPLAIDPGDDPVTISIGGISDVDGAGSWISYGTEDYPSGFVIHAD"
     gene            4107..7910
                     /locus_tag="VEV43_12625"
     CDS             4107..7910
                     /locus_tag="VEV43_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP24411.1"
                     /translation="MRHALRRRVHAISVLAVIALVSGLVAAGPVRAAATAGPAPVDRL
                     VSEATGRASALVHVEPAASLRDGLAAARAAGLETGTVYDAIKVFVAYGSAAEFGELAQ
                     AEAIQYVEANRRVKLATNSSHRATRGQDVLDGAVTVGGTKIDGRGVGVAVIDSGVDGT
                     HPDLASRMGGNVKIVCTSPLPATNAFLGGFQECRTPKDVVELDDTDHPSAGGHGTHVA
                     GIVAGTGSTYHGAAPGATLYGVSVGTTILVENGLDGLAWVLDNHDQVSPAIKVVNNSW
                     GSGYRKYAANGDVLYSATWKLQEELIRAGVTVVFAAGNAGGNGTNPTTVAECVNPTPG
                     LVCVANYDDKGNGTRAGTIDSTSSRGNTTDVESWPDVSAPGTVITSTCRVTLPVCNMH
                     FGMVVNPPNMYASLSGTSMSAPHVAGIVAQLYQADPTLTPAEIEIVLEDTAHKFQWGS
                     AYGLRTDPTNPDNGTSFEKGHGLVDALAAVQAVLGGSGGTSTEPTTPPSESPEPTATP
                     TETPAPTTPPTESPTTGGTRYYFHSASGMGNADLFLADGHPFDTNPPTLDSYSEYHDL
                     PDTNGAPNAIYDPNWAGEIPGTVTSITLDFWAKSPVGDVLDEVNFDPYVWVGETRYAL
                     PTMTGTPEAASIGNVPSRITHTYTTMLDDAGNEVPLSIPTGGQPVTVAISGHFTDTDA
                     GSWIVYDSTDYPSGFVVNGGDGTDPTESPSPTPTVSPTVEPTNEPTPTPAPGRGTYPV
                     EPDDPMFDLQWGMTKIQAPEAWQERNATGFGINIAIVDSGVDLGHPDFQCDKLRVLPG
                     SDVSSGDDDPQDTDGHGTHVAGIAGACTDNGTGVVGVAPDATLIPVRATGGAADLDQS
                     MADGIRFATDNGAHVVNLSIGDIPPFSHFGPDGFPETEEAMQYARDNGVVIAAAAGNF
                     AQPTCEYPSLSRNVICVVATDRDDLRAWYTDFPVNVDRNSDEPGLEPVVAAPGGDGLF
                     CDGGVTSTYLRSAASTCHDSGYDSLHGTSMSSPHVAGIAALLYDRLGGERSKANADLV
                     VQTILDTADDLYSPGWDPIVGYGRVNALSAVQAIDVAEPSATQVAFTDNTSETVQYSD
                     PATFEAELRDTVTGEPIPGAQIVFRMAGAEAAQEFVALTNEQGIASVRYESVGETPGA
                     YDLTARFLGEGEALLGSADVRSLLVEKETSALALTVQKDKTSGKTATATLTDADAAAG
                     AGGLGGRMVEFFAGDRSLGFVMTNDQGVATIDVKQKNAPAGQGFTAVFAGDDLYQGSN
                     ASANG"
     gene            8005..8077
                     /locus_tag="VEV43_12630"
     tRNA            8005..8077
                     /locus_tag="VEV43_12630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8038..8040,aa:Arg,seq:cct)
     gene            8188..>9052
                     /locus_tag="VEV43_12635"
     CDS             8188..>9052
                     /locus_tag="VEV43_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYP24412.1"
                     /translation="MASIKKVPRKRKDGSRSHAWKVRYRDPDRVERSRTFRTEADARR
                     FASKIEVSVNEGTYIDPSLGRTLFKDWAGDWLEASEPALKPHTISGYRTLLRKHVIPA
                     LGSTQLAKVRALDVRRFVAELSNAGLSASRVRSTYFLVQATFRAAVESGYIGRSPCVG
                     VKLPRSVVREMNFIGADQVKAIGAAVPEQYEALIHLLAFGGLRWGEAAALRRKHVNVL
                     RRRVEVAESLADVDGQLHFGPTKTHQTRVVTLPKKTNEVLRLHLERFVEPDRDALVFT
                     SPQGDALRSPNF"
BASE COUNT         1513 a         3259 c         3048 g         1232 t
ORIGIN      
        1 gaagtacggg atgccgcagg acgagtaaga ggtgaagtgg ccgcgctcga aagcgacgat
       61 gtcgaggtcg tccggccccc gcaatcgccg cgcctgactc gccgcggtca tgccacccgc
      121 gtcgccgcct acgaccacca accgttccgt cattcgttgt ccttgcttcg actgcttgcc
      181 ggggccgcgg gtcaaatccc gcccgaatgg gacattaagt ggaggaatta cgggtgctgg
      241 ccccgaaaca ttcccacagc cagttcgagt agaggggtgg tcgcaatgca ggtcgaagac
      301 aaggtctgtc acgagctcaa ggaagggctg acgctcgcct tgggttggtt ggagacgttg
      361 ttccagaggt ggcagacgct gcccgacgca cagcgccagc agatggtcgc tggcgccctc
      421 ctcggcgtga accgtgcgac cttcgcgctc gaccagatcg aggggaagtc cgtcgaggac
      481 atcgcgtccc cggaggagcg catggcgagc gagctcgtca agctcgtcga ggacccgatc
      541 gagatcgcgg cagggaacgg cgcgcttcgc tgacgcgtcc cgtgcccctc ggggacagct
      601 gagcaagcag acatttcgga catttcacgc aggactttcg aggtcggtaa agaaaaacgt
      661 gactgcggcg cacctccccc cacgcgccgg gagcccgtcg tccgaccgaa aggatcgacc
      721 atgcgcgtct tcccgaaacg ccgcctcgta gcgctctctg cgctcgtcgc caccgccctg
      781 agcctcgggc tcaccggagc gggggcccag accactacgc cgaacgaccc gctgttcgcg
      841 aaccagtggg gtctccagaa gatccaggcc cccgcggcat ggtcgctggc ggacggaacg
      901 ggcatcacga tcgcgatcgt cgactcgggc gtcgacctcg gccacgacga cttcgcgtgc
      961 ggcaagctcg ccgtgcaccc gggctcgaac atcgggtcgc gcaagagccc gaacaccccg
     1021 caggacgcgg atggccatgg cacccacgtc gccgggatcg ccggcgcgtg cacgaacaac
     1081 ggcgtcgggg tggccggcgt cgctccgggc tcgacgatca tgccggtgtc ggtgttcaac
     1141 gagtcggacc tcgacaaggc gatggccgac ggcatccgct tcgcgaccga ccgcggcgca
     1201 cacgtgatca acctctcgat cggcgacatc ccgccgttca gccacggcgg tgcgctcctg
     1261 ttcccgcaga ccgaggacgc gctggagtac gcgcgcaact ccggtgtcgt catcgccgcc
     1321 gcggcgggca acttcgacca gccgacgtgc gagtacccgt cgttctcccg caacgtgatc
     1381 tgcgtcgtcg ccacggacag caacgacgtg cggtcgtact acagcgactt ccccgtgaac
     1441 ggtgacccgc agaccctgcc ggcgatggag ccggtcgtcg cggcccccgg gggccagggc
     1501 acgttctgcg acgagggcat cgtctcgacc tacctgcgca gcctcgactc cgtctgctac
     1561 tccacgggct acgagagcct cgacgggacc tcgatgtcct ctccgcacgt cgccggcgtc
     1621 gcggcgctcg tgtaccagag gctcggcgga gtgcgctccg cggcgaacgc ggacctcgtc
     1681 gtgcagacga tcctggactc ggcggacgac ctctacacgc cgggttggga cccgatcgtg
     1741 ggtcacggcc gcgtgaacgc cctcaccgcg gtgcagtccg tcccgtaaga cacgcgtcgt
     1801 ccacgagaac gcccccgcta tcccgcgggg gcgttctttg ttgtcgggcc ctcgacgttc
     1861 cgggtcgcag tcgcaggagc ggaccaagcg cggcagaacc cccgctctca ccatcggtcc
     1921 ccactgcacg ggaggaacgc catgccccgc cgcttccaca cgatctcgct cgtcgcgctc
     1981 gcgtccctcg tctgcgcggt cctggccgcg atccccgtcc gcgcgggagc cggcgagggt
     2041 cccgcgccgg tcgatcgtcc ggtgctgcgc gcgacgaggc gaaccagcgc gctcgtccac
     2101 gtcgtcgaag gcacggccct tcgtgacggg atcgcagcgg cgcgtgcggc cgggctcgag
     2161 accgggaccg tgtacgacgc gatccgcgtc ttcgtcgcct acggctcggc gaccgagttc
     2221 tcggagctcg cgaaggcccc atccgtcgga tacgtcgagg cgaaccgccg gatgcgtctc
     2281 ttcagcgaca cgtcccatgg ggccaccagg ggccaggacg tcctcgacgg agccgtgacg
     2341 cttgccggcg gggccacgat cgacggcacc ggcgtcggcg tcgcagtggt ggacaccggc
     2401 gtcgacggga cccatccgga cctcgcgccg cgcatgggag gcaacgtcaa gatggtctgc
     2461 accgtgccgc tgatcgtcaa cgggcccgag gacgggttca ccgaatgccg cgggccgaag
     2521 accgcggtcg agctggagga caccgaccac gcctccccca gcgggcacgg aacgcacgtc
     2581 gcgggcatcg tcgccgggac cggcgaggca tcggccggcg agtaccacgg ggccgcgccg
     2641 ggggtcacct tgtacggcgt gggcatggga accgtcgtgc tcccggagaa cgcgctcgac
     2701 gggctcgcat gggtcctcgc gaaccacgac caggtgagtc ctccgatcaa ggtcgtcaac
     2761 acctcctggg gcaccagcta ctcgaagtac cggccgtccg gagatccgac ctacagcgcg
     2821 acgtggaagc tccaggagga gctcgtggaa gcgggcatca ccatcgtcgt gtcggccggc
     2881 aacggggccg gcaccgggac ggccgcgacg acgagcgcgc agtgcgtgaa ccccacgccc
     2941 gggatcgtct gcgtcgcgaa ctacgacgac cagggaacgg ggacgagggc ggggacgatc
     3001 gaccccggct cctcgcgggg gagcacctcc gacgtagaga gctggcccga cgtgtcggcg
     3061 cccgggacgc tcatcacgtc gacttgccgg atgacgcttc cgatctgcca cctccacgac
     3121 ggaatgctgc tggatccgcc gaaccgctac gcgacgctca cggggacttc gatggccgcc
     3181 ccgcacgtcg cggggatcgc ggcgcagctc tatcaggcag acccgtcgat cacgccggcc
     3241 gagatcgaga acgtcctcga ggacacggca cacaagttcg cgttcggagc cgcgtacggc
     3301 ctgctgaccg acgccacgaa cccggacgac acgtcgtcgt acgagaaggg acacggcctc
     3361 gtggacgccc tcgccgcggt cgaggcggtg ctgaacgccg ggacgacccc gaccccctct
     3421 ccgagcccga cggcgacgcc gaccgcgccg cccccctcgg ggacgaggta ctacttccag
     3481 accgcaacct gggtgggcga ggccgacggc gtctcgagcg gcagcacgtt cgacagcgag
     3541 accccggccg ccctgatcta cgcggagtgg cgcgacctcc cgcgctcgaa cgagcggccg
     3601 gcggacccgt tcgaccctca ctggacggga cgggtcgacg gaccgatcga gggcctcacg
     3661 ctcgacttct gggcgaagac gcccgtcggg gaggagctcg gcaccgtgag ctatcgcccg
     3721 gtggtgtggg tgggagacga cccgtacgtc ctgccgacac tcaccgtccc gatgcagccg
     3781 tcgctgggca acgtccccac gcggctcacc cggagctaca cgacgaggct cgacgcggcc
     3841 ggcaacgagg tcccgctggc gatcgatccc ggcgacgacc ccgtcacgat ctcgatcggg
     3901 ggcatctcgg acgtcgacgg ggccggcagc tggatctcct acgggaccga ggactacccg
     3961 tccgggttcg tgatccacgc cgactagcgg gagctttacg caggagattc ggcgccggtc
     4021 cagaacaagc tccgggtgag gcggcggcgg atcacgtcag agcctgccca cgccattggg
     4081 gcccgtacgt aatggggagg aactgaatgc gccacgcgct gcgccgccgc gtccacgcga
     4141 tctcggtact agccgtcatc gccctcgtct ccggcctcgt ggccgccggc cccgtccggg
     4201 ccgccgccac ggcaggccct gcccccgtcg accgtctcgt cagcgaggcg accgggcgag
     4261 ccagcgcgct cgtgcacgtg gagccggcgg cgtccctccg ggacgggctc gccgcggccc
     4321 gcgccgccgg gctcgagacc ggcacggtct acgacgcgat aaaggtgttc gtcgcgtacg
     4381 ggtcggccgc cgagttcggc gagctcgcgc aggcggaagc gatccagtac gtcgaggcga
     4441 accgccgcgt caagctggcg accaactcct cccacagggc gacccggggg caggacgtcc
     4501 tcgacggggc cgtcaccgtc ggcgggacga agatcgacgg ccggggcgtc ggcgtggcgg
     4561 tcatcgactc cggcgtcgac gggacgcacc ccgacctcgc gtcgcgcatg ggcgggaacg
     4621 tcaagatcgt ctgcacgtcc ccgctccccg ccacgaacgc cttcctgggc gggttccagg
     4681 aatgccggac gccgaaggac gtagtcgagc tcgacgacac cgaccacccc tccgcgggcg
     4741 gtcacggcac ccacgtcgcc ggcatcgtcg ccgggacggg ctcgacgtac cacggtgcgg
     4801 cgccgggcgc gacgctctac ggcgtcagcg tcgggaccac gatcctcgtg gagaacggcc
     4861 tcgacggcct cgcctgggtg ctcgacaacc acgaccaggt cagccccgcc atcaaggtcg
     4921 tcaacaactc ctggggcagc ggctaccgca agtacgctgc gaacggcgac gtcctctaca
     4981 gcgctacgtg gaagctccag gaggaactca tccgcgcggg cgtgaccgtc gtcttcgccg
     5041 ccgggaacgc gggcggcaac ggcacgaacc cgacgacggt cgccgagtgc gtgaacccga
     5101 cccccggcct cgtctgcgtc gcgaactacg acgacaaggg caacgggacc cgggccggca
     5161 ccatcgactc gacctcgtcg cgcggcaaca ccaccgacgt cgagagctgg ccggacgtgt
     5221 cggcccccgg gaccgtcatc acgtcgacgt gccgcgtcac gctgccggtc tgcaacatgc
     5281 acttcgggat ggtcgtgaac ccgcccaaca tgtacgcgag cctgtcgggc acctcgatgt
     5341 ccgcgcctca cgtggccggg atcgtcgctc agctctacca ggcggacccg acgctgacgc
     5401 ccgccgagat cgagatcgtc ctcgaggaca ccgcgcacaa gttccagtgg ggaagcgcgt
     5461 acggcctccg gacggacccc acgaacccgg acaacggcac ttcgttcgag aagggccacg
     5521 ggctcgtcga cgcgcttgct gcggtgcagg ccgtgctcgg tggtagcggc gggacgtcga
     5581 ccgagccgac cacgcctcct tcggagtcgc ccgagcccac cgcgacgccg accgagaccc
     5641 cggccccgac gactcctccg accgagagcc cgacgaccgg cgggacgcgc tactacttcc
     5701 actccgcctc gggcatgggc aacgccgacc tcttcctcgc cgacgggcac ccgttcgaca
     5761 cgaacccgcc gacgctcgac agctactccg agtaccacga tctccccgac accaacggcg
     5821 cgccgaacgc gatctacgac ccgaactggg ccggcgagat ccccgggacc gtcacgagca
     5881 tcacgctcga cttctgggcg aagtcgccgg tcggcgacgt cctcgacgag gtcaacttcg
     5941 atccctacgt ctgggtcggc gagacccgct acgcgctgcc gacgatgacc ggcacgcccg
     6001 aggcggcgtc gatcggcaac gtcccgagcc gcatcacgca cacgtacacg acgatgctcg
     6061 acgacgccgg caacgaggtc ccgctgtcga tcccgaccgg gggccagccg gtgacggtcg
     6121 cgatcagcgg ccacttcacc gacaccgacg cggggtcgtg gatcgtctac gactcgaccg
     6181 actacccctc cggcttcgtc gtcaacggcg gcgacgggac cgacccgacg gagtcgccga
     6241 gcccgacgcc caccgtctcc cccaccgtcg agccgacgaa cgagccgacg ccgacgccgg
     6301 cccccggccg cgggacgtac ccggtcgagc cggacgaccc gatgttcgat ctccagtggg
     6361 gcatgacgaa gatccaggcg ccggaggcgt ggcaggagcg caacgcgacc ggcttcggca
     6421 tcaacatcgc gatcgtggac tccggcgtcg acctcggcca ccccgacttc cagtgcgaca
     6481 agctgcgggt gctgcccggc tccgacgtga gctccggtga cgacgacccg caggacacgg
     6541 acggacacgg cacgcacgtc gccggcatcg cgggtgcgtg caccgacaac gggacgggtg
     6601 tcgtcggcgt cgctcccgac gccacgctca tccccgtgcg cgcgaccggt ggcgccgccg
     6661 acctcgacca gtcgatggcc gacggcatcc gcttcgcgac ggacaacggt gcacacgtcg
     6721 tcaacctctc gatcggcgac atccctccgt tcagccactt cggtcccgac ggcttcccgg
     6781 agaccgagga ggcgatgcag tacgcacgcg acaacggcgt ggtcatcgcg gcggccgccg
     6841 gcaacttcgc gcagccgacg tgcgagtacc cgtcgctgtc gcgcaacgtg atctgcgtcg
     6901 tggcgaccga tcgcgacgac ctgagggcct ggtacacgga cttcccggtg aacgtcgacc
     6961 gcaactcgga cgagccgggg ctcgagccgg tagtcgcggc ccccggtggt gacggcctgt
     7021 tctgtgacgg cggggtcacc tcgacgtatc tccgctcggc tgcatcgacc tgtcacgact
     7081 ccggctacga cagcctccac ggcacgtcga tgtcgtcgcc gcacgtcgcc ggcatcgcgg
     7141 cccttctcta cgaccgcctc ggcggcgagc gctcgaaggc gaacgcggac ctcgtcgtgc
     7201 agacgatcct cgacaccgcc gacgacctct actcgccggg ctgggacccg atcgtcggat
     7261 acggacgcgt gaacgcgctg tccgcagtcc aggcgatcga cgtggcggag ccgagcgcga
     7321 cgcaggtcgc gttcaccgac aacacctccg agaccgtcca gtactccgac cccgcgacgt
     7381 tcgaggccga gctgcgcgac accgtgaccg gcgagccgat ccccggcgcg cagatcgtgt
     7441 tccgcatggc gggggccgag gccgcgcagg agttcgtcgc gctgacgaac gagcagggca
     7501 ttgcatcggt caggtacgag agcgtcggcg aaacgccggg cgcgtacgac ctgaccgcac
     7561 gcttcctggg cgagggcgag gcgctgctcg gctccgccga cgtgcgctcg ttgctcgtcg
     7621 agaaggagac gtcggcgctc gcgctgacgg tgcagaagga caagacgtcc ggcaagaccg
     7681 cgacggccac gctgacggac gcggacgcgg ctgcgggggc cggcggcctc gggggccgca
     7741 tggtcgagtt cttcgcaggc gaccggtcgc tgggcttcgt gatgacgaac gaccaaggcg
     7801 tggcgacgat cgacgtgaag cagaagaacg cgccggccgg ccagggcttc accgcagtct
     7861 tcgcgggaga cgacctctac caaggctcga acgcgtcggc gaacggctga cgggtccggc
     7921 ccgcaggggt ctctaacgca ggagccggtc cgggggcgga ggaccggctc ctgcgctttg
     7981 cgggcttgtc tagactcggg ccgcgccctc gtagctcagg ggatagagca caggtttcct
     8041 aaacctggtg tcgcaggttc gaatcctgcc gggggcaccc aagaaaccgc aggtcagagg
     8101 caacatcttg ccttacgcca tcccgccacg tacgcttgat cggcggaaaa gtccgcaaaa
     8161 agtccgcaag gcctcaggcg gtggcgagtg gcatcgatca agaaggtccc ccggaaacgc
     8221 aaggacggct cgcgcagtca cgcctggaaa gtccgctacc gcgaccccga ccgcgtcgag
     8281 cgaagccgca ccttccgcac cgaggccgac gcgaggcgct tcgcgagcaa gatcgaagtc
     8341 agcgtcaacg agggcaccta catcgacccg tcgctaggtc gaaccctgtt caaggactgg
     8401 gcaggcgact ggctggaggc aagcgagccg gcgctgaagc cccacacgat ctcgggctac
     8461 aggacgctgc tccggaagca cgtcatcccc gcgctgggat cgacgcagct ggctaaggtg
     8521 cgagcgctcg acgtaaggcg ttttgttgcg gaactttcca acgctggcct gagcgcctca
     8581 cgcgtccggt cgacgtactt cctcgtccag gcgaccttcc gcgccgccgt cgaatccggc
     8641 tacatcgggc gctccccttg cgtcggcgtc aagctccctc gcagcgtcgt acgcgagatg
     8701 aacttcatcg gtgcggatca agtgaaagcg atcggggcgg ccgtaccgga gcagtacgaa
     8761 gcgctgatcc acctgctggc gttcggcggc ctgaggtggg gcgaagccgc tgctctgcgc
     8821 aggaagcacg tcaacgtcct gcgccggcgg gtggaagtcg cggagtcgtt agcggacgtc
     8881 gacggccagc tccacttcgg cccgacgaag acacaccaga cccgcgtcgt cacgctgccc
     8941 aagaagacga acgaggtgtt gcgcctccac ctggagcgtt tcgtcgagcc ggaccgggac
     9001 gcgctcgtgt tcacctcgcc acaaggagac gcgctgcgct ctcccaactt cc
//
LOCUS       DASQDO010000462         8288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_783655, whole genome shotgun sequence.
ACCESSION   DASQDO010000462 DASQDO010000000
VERSION     DASQDO010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783655"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VEV43_12640"
     CDS             <1..586
                     /locus_tag="VEV43_12640"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HYP24413.1"
                     /translation="DVVLAAVAGALRRLMKARGERVDDLVLRAMVPVSVRAEADRGAL
                     GNRVASMWAPLPVYEPDPRRRLEKITETMGDLKQSGQAVGAQVLASLGEYAPPTIVAQ
                     AARLVARQRAFNLVVTNVPGPQIPLYTLGREMSEVYPVLPLADNTTIGVALLSYNGTI
                     GFGLLGDYDTAPDIAVLAEGIEKSIAELSAVAAV"
     gene            590..892
                     /locus_tag="VEV43_12645"
     CDS             590..892
                     /locus_tag="VEV43_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24414.1"
                     /translation="MSQGPELGADPPPRPEPPPPSDAARIIDAITDARQTIVRTIVVA
                     TLLILMAIAYAYCRDPHSECRALVEKERGPEGLSDEDLATWDELRCGRFAASPLSR"
     gene            complement(873..1535)
                     /locus_tag="VEV43_12650"
     CDS             complement(873..1535)
                     /locus_tag="VEV43_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HYP24415.1"
                     /translation="MNSKLFDSRVSRLRDATVLAIFRTRAPQHAVEGIGAAVRGGFEA
                     VEITMNSPGATEALAEVAGRVDAIVGAGTITHPDQVKEAYDAGAEFIVTPIVIPEVVE
                     AAHGLAMPIAMGASTPTEIFTARSAGADWVKVFPGESLGGPEYITNVLGPLDGTPILV
                     TGGLTPENYLGYLDAGAELVGFANAVFEPELAAEGRYDEMERRAIEVCRRLDLYFTEK
                     ED"
     gene            complement(1545..3968)
                     /locus_tag="VEV43_12655"
     CDS             complement(1545..3968)
                     /locus_tag="VEV43_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HYP24416.1"
                     /translation="MKPVSLTIKGFRSHGEATELSFVDRRLFAIVGPTGAGKSSILDA
                     VSYSLYGATPRIKRNVKRLISTRADSAHVRFAFEVEGHEYEISRSLRRSGPGEHVLVD
                     GSSGQRVIGEAKVTERVTQLLGLDFDAFCSSVLLAQGRFAKFLEAPPTERSQILKGVF
                     KLDQIDALREEAKARRNELDGELKRVEGKKAHIPDDVETRLEEAIATHGTCTSRVKVL
                     EKAVPDERKLAVTVERAAEALTAAEASLKSARGALAAAPTPDDLRELAEREEEVAEPL
                     AAAEAAAADATEERSKAQEDLAALEEELGAHSALIEARVRARSLAEAASLLTTHTVAV
                     EVAGKKLEAATSALTEAEAFEHQALESLEKIRAEREEIERAHQAHVLREVLVVGEPCP
                     VCEQSVDALPRGKRPAALSGNRKAEKEAASTHDKATKATRAAAAAQASAERDLAAAQA
                     RLADEEQKLEASRGAMAELVGDAADPLAEVERRLDLVSAGREAIAAANESVERARAEL
                     TRWTGAKAKVTAVASKLVAQLSHVAGALSLPAPSHDAPVADLVDEASAVVRAAEAAVA
                     GAESAAEKARAAEGEARTALAELRASLDLKASQSIEAVLREAIRTATEAEGRERSLRE
                     MVAEAAKLDEQERQFRASLALYVQLADDMTDKNFIKFMLEEKRRLLSDIGSERLREMT
                     RRYRFDDRGEFNVVDELDADTERTVDTLSGGETFLASLALSLALAEAVTRHGGRLQCF
                     FLDEGFGSLDPESLDAALEGIEHIVTDDRLIGLVSHVEALRNRIEDKIVLDKDDDGMT
                     RVIAGSTLG"
     gene            complement(3965..5125)
                     /locus_tag="VEV43_12660"
     CDS             complement(3965..5125)
                     /locus_tag="VEV43_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HYP24417.1"
                     /translation="MRILHTGDWHSGKKLGRIDRAAEAEAVLDEVVAIADDEDVDAVL
                     VAGDLFDRAAPPLPYLSLVLESLRRLASNDRRVVVIPGNHDSPELMRVLGPLLAGIGV
                     YVVHKPLAPGAGGVVTVPARDGSMAARVACLPFVHEAQVIDVMDAPAEGYKSYADRIR
                     ALTSHYAGWMVANPEPKAADLLMGHFMVHGAVPSGTERELHIGEAYMATAEAIPSEIK
                     YAALGHIHQAQDAPGSAVPARYCGSLLQLDFGEAGQEKSVCVVEVSPGMKPARARCVP
                     VTSGRPLMKVTGTIDDLAARAGEFGDAILDVSVITDGPAPGLADEVRTLLPHALYVRA
                     AWERQELAESTREGKTLQELYADYYQERHAAPPPPELVHAFEDLLDRVGVSL"
     gene            complement(5130..6863)
                     /locus_tag="VEV43_12665"
     CDS             complement(5130..6863)
                     /locus_tag="VEV43_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP24418.1"
                     /translation="MTDREVGLVLGSEPTSTQEFRVVLHEDEYLQVDDLVVVYTHVPK
                     VGEVATYGIVIETAAVYEGASFESDTFRVAVEGTLPAEKSRTATVQTVRVLPETWVAP
                     DSGQPVFKATGAERERALYVDEMGGGESARALPVGLSRDDEPVWVDVDFFDGTKGAHM
                     SISGVSGVATKTSYALFFLRCLTAHPKSLGKGSRNLKVLVFNVKGQDLLFLDKPNVRF
                     TEEAADDWRKLGMDPAPFDSVSFWAPAMSHAGSEGIPDSRARTEGVEAFRWTPREFVD
                     EHLLPFLFTDAADTRQQITFVAERVTRQLERFACDVAGRPGAIVLRDPTDAIHSDEPV
                     VPFPGERVIESLGDLVEEIATYVEPDDGEPDLAWTSRVAQGTVSAFMRRLYGALPRVG
                     RLVKAGPSRRIDRAREAVTVVDIHDLHDAAQRFVVGAMIAEVHREKEGRGRFPLSVIV
                     LDELNKYAPREGWSPIKDTLIDVAQRGRSLGILLIGAQQSASRIDPDILGNASIKVSG
                     RLDSAEAERAEYGWMLPSTRARARLLKPGTMVLSQPSIPAPTVLEFPYPPWATRPEEV
                     DDSGADPFEGL"
     gene            complement(6860..7828)
                     /locus_tag="VEV43_12670"
     CDS             complement(6860..7828)
                     /locus_tag="VEV43_12670"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HYP24419.1"
                     /translation="MRIAVEAWSHEYAAEVDLGGPDDFSVEDVRTDHETRGEPWEPVP
                     RSPDAPGVETVAFVDGTRRVDARLFGMNGGDMPVPGLAGSIGVGAVVCEPSPDGCAAR
                     ITTETITRWLVLGGGHAQGLYAGPALSYEPMPHPADTTDVLFQALQEHMRRLEANLAV
                     AAADQGHLVFADGPLAKMDPGKRKVIGLIKSHSVRYLKSPEEDVLARLGCGERTPLFS
                     FGEKRPRYSWYVRLCERDAGGHAWQGLVRCEVPAALSVEAAIELANASTALLPRYASK
                     PYWDKRAPQNLVPIAALEKRLRHLLGEPALVYRQIRSAAARAEVPA"
     gene            complement(7902..>8288)
                     /locus_tag="VEV43_12675"
     CDS             complement(7902..>8288)
                     /locus_tag="VEV43_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24420.1"
                     /translation="AWHPHPVTFPSNLLLPLYASVRRILEAGVQEWVERRADLAKRCR
                     EGLRALGLEPVPQAGAEANLVVAAWADDPASIQKHLLANSGIMISGGLDPTAGKAIRV
                     GLMGATATAEMVDKVVAGVEAALAVR"
BASE COUNT         1156 a         3003 c         2876 g         1253 t
ORIGIN      
        1 cgacgtcgtg ctcgccgcgg tggccggcgc gctgcggcgt ctgatgaagg cgcgcggcga
       61 gcgcgtcgac gacctcgtgc tgcgcgcgat ggtgccggtg tcggtgaggg ccgaagccga
      121 ccggggcgcg ctcggcaacc gcgtcgcgtc gatgtgggcg ccgttgccgg tgtacgagcc
      181 ggacccgcgg cgcaggctcg agaagatcac cgagacgatg ggcgacctca agcagtcggg
      241 ccaggccgtg ggggcccagg tgctggcgag cctcggcgag tacgcgccgc cgacgatcgt
      301 ggcgcaagcc gcacgtcttg tcgcgcgcca gcgcgccttc aacctcgtgg tgacgaacgt
      361 gccgggccca cagataccgc tgtacacgtt gggccgggag atgagcgagg tctacccggt
      421 gctgccgctg gccgacaaca cgacgatcgg cgtggcgctg ctctcgtaca acgggacgat
      481 cggcttcggc ctgctgggcg actacgacac ggcgccggac atagcggtac tggccgaggg
      541 gatcgagaag tcgatagcgg agctgtcggc ggtagccgcg gtctaacgca tgtcgcaggg
      601 accggagctg ggtgccgacc cgcccccgcg gcccgagccg cctcccccca gtgacgccgc
      661 gaggatcatc gacgcgatca cggacgcgcg acagacgatc gtccgcacca tcgtcgtcgc
      721 gacgctgctg atccttatgg cgatcgccta cgcgtactgt cgagaccctc actcggagtg
      781 ccgcgccctg gtcgagaaag agcgcggccc cgagggcctg tccgacgagg acctcgcaac
      841 gtgggacgag ctgcggtgcg gcaggttcgc cgctagtcct ctttctcggt gaagtagagg
      901 tccagacggc ggcagacctc gatcgcccgg cgttccatct cgtcgtagcg gccctccgcc
      961 gcgagctccg gctcgaacac cgcgttcgcg aagcccacca gctcggcccc cgcgtccagg
     1021 tagcccaggt agttctccgg cgtcagcccg ccggtgacca ggatgggcgt cccgtcgagg
     1081 gggcccagga cgttcgttat gtactccggc ccgccgaggc tctcgcccgg gaagaccttc
     1141 acccagtcgg cccccgccga gcgcgccgtg aagatctcgg tcggcgtcga cgcgcccatc
     1201 gcgatcggca tggcgaggcc gtgggcggcc tcgacgacct cggggatcac gatcggcgtg
     1261 acgatgaact cggcaccggc gtcgtacgcc tctttgacct ggtcgggatg cgtgatcgtg
     1321 ccggcaccga cgatcgcgtc gacgcgcccg gccacctcgg ccagcgcctc ggtcgcgccc
     1381 ggcgagttca tcgttatctc gacggcctcg aacccgccgc gcaccgcggc cccgatcccc
     1441 tcgaccgcgt gctggggagc gcgcgtgcgg aagatcgcga gcacggtcgc gtcgcgcaac
     1501 cggctcaccc gcgagtcgaa gagcttcgag ttcatcgacg caggttaacc gagcgtcgaa
     1561 cctgcgatca ccctcgtcat gccgtcgtcg tccttgtcga gcacgatctt gtcctcgatc
     1621 cggttgcgca acgcctcgac gtgcgacacc aagccgatca agcgatcgtc ggtgacgatg
     1681 tgctcgatcc cctcgagcgc ggcgtcgaga gactcggggt cgagcgagcc gaatccctcg
     1741 tcgaggaaga agcactgcaa gcgaccgccg tgacgcgtca cggcctccgc gagcgcgagc
     1801 gacaaggcga gcgaggcgag gaacgtctcg ccgcccgaca acgtgtcgac cgtgcgttcc
     1861 gtgtcggcgt cgagctcgtc gacgacgttg aactcgccgc ggtcgtcgaa tcgatagcgc
     1921 cgcgtcatct cgcgcaagcg ctcggacccg atatcggaga gcagcctgcg cttctcctcg
     1981 agcatgaact tgatgaagtt cttgtcggtc atgtcgtcgg cgagctggac gtagagcgcg
     2041 agcgacgcac ggaactgcct ctcctgctcg tcgagcttcg ccgcctcggc caccatctcg
     2101 cgcagagagc gttcgcgccc ctcggcctcc gtcgccgtgc ggatcgcctc gcgtagcacg
     2161 gcctcgattg actgcgacgc cttcaagtcc agcgacgcgc gcagctcggc cagagcggtc
     2221 cgcgcctcgc cctcggccgc ccgcgccttc tcggcggccg actcggcgcc ggcgactgcg
     2281 gcctcggccg cccggaccac ggcagacgcc tcgtcgacga ggtccgctac cggcgcgtcg
     2341 tggctggggg ccgggagcga cagcgcgccg gcgacgtggc tcagctgcgc aaccagcttg
     2401 ctcgcgaccg ccgtcacctt cgccttcgcg ccggtccacc tcgtcagctc ggctcgtgcg
     2461 cgctcgacgc tctcgttcgc ggcggctatt gcttcgcgcc ctgcgctgac gaggtccagc
     2521 cgtcgctcga cctcggccag cgggtccgcc gcgtcgccca ccagctcggc catcgccccc
     2581 cggctcgctt cgagcttctg ctcctcgtcc gcgagcctcg cctgcgccgc ggccaggtcc
     2641 cgctcggcgg atgcctgagc cgcggccgcc gcgcgcgtag ccttggtcgc cttgtcgtgc
     2701 gtagaagcgg cctccttctc ggctttccga ttgccagaga gcgcggccgg gcgcttcccc
     2761 cgcggcagcg cgtcgacgct ctgctcgcac acggggcacg gctcgccgac gacgaggacc
     2821 tcgcgcagca cgtgggcctg atgggcgcgc tcgatctcct cgcgctcggc ccggatcttc
     2881 tcgaggctct ccaacgcttg gtgctcgaac gcctcggctt cggtcagcgc gctcgtcgcg
     2941 gcttctagct tcttcccggc gacctcgacc gcgacggtgt gcgtcgtcag caacgacgcg
     3001 gcctcggcca gcgaccgggc ccgcaccctc gcctcgatca atgcggaatg cgcgccgagc
     3061 tcttcctcga gcgcagcgag gtcttcttgg gccttggatc gttcctcggt agcgtctgca
     3121 gccgcggcct cggccgccgc aagaggttcc gcgacctcct cctcgcgctc ggccagctcg
     3181 cggaggtcgt ccggtgtggg ggccgcggcg agcgcaccgc gggcggactt gagcgacgct
     3241 tccgcggcag tcagggcctc ggccgctcgc tcgaccgtga ccgcgagctt gcgctcgtcc
     3301 ggcacggcct tctcgagcac cttcacccgg ctcgtgcacg tgccgtgcgt cgcgatcgcc
     3361 tcttcgaggc gcgtctcgac gtcgtcgggt atgtgggcct tcttgccctc gacgcgcttc
     3421 agctcgccgt ccagctcgtt gcgccgcgcc ttcgcctctt cccgcagcgc gtcgatctgg
     3481 tcgagcttga acacaccttt gaggatctgc gaacgctccg tcggcggtgc ctcgaggaac
     3541 ttcgcgaagc gtccctgcgc gagcagcacg gacgagcaga acgcgtcgaa gtcgagcccg
     3601 agcagctgcg tcacccgctc ggtgaccttc gcctcgccga tcacgcgttg acccgacgag
     3661 ccgtcgacga ggacgtgctc gccggggccg cttcggcgca ggctgcgcga gatctcgtac
     3721 tcgtggccct cgacctcgaa ggcgaaccgg acgtgcgcgg agtcggcccg agtcgagatc
     3781 agccgcttga cgttgcgctt gatccgcggc gtcgcgccgt acagcgagta cgagaccgcg
     3841 tcgaggatcg aggacttccc ggcccccgtg ggaccgacga tcgcgaagag ccggcggtcc
     3901 acgaacgaca gctcggttgc ctcgccgtgg ctgcggaacc ccttgatcgt gagcgacacc
     3961 ggcttcacag cgacaccccc actcggtcga ggaggtcttc gaacgcgtgc accagctcgg
     4021 gaggcggtgg cgcggcatgg cgttcctggt agtagtcggc gtagagctcc tgcagcgtct
     4081 tgccctcgcg ggtcgattcg gcgagctcct gacgttccca cgcggcccgc acgtacaagg
     4141 cgtgcgggag gagtgtccgg acctcgtccg cgagcccggg cgccggcccg tcggtgatca
     4201 cagagacgtc gaggatcgcg tcgccgaact cgccggcccg agcggcgagg tcgtcgatcg
     4261 tccccgtcac cttcatcagc ggtcggccgg acgtcaccgg gacgcagcgc gcccgcgcgg
     4321 gcttcatccc cggcgacacc tcgacgacgc agactgactt ctcctgcccc gcctcgccga
     4381 agtcgagctg gagcaggctc ccgcagtagc gcgcggggac cgcggaaccg ggcgcgtcct
     4441 gggcctggtg gatgtggccg agcgcggcgt acttgatctc ggaagggatg gcctcggcag
     4501 tcgccatgta ggcctcgccg atgtgcagct cgcgctcggt cccggacggc accgcgccgt
     4561 gcaccatgaa gtgccccatg agcaggtcgg cggccttcgg ctcggggttc gcgaccatcc
     4621 accccgcgta gtgcgacgtg agcgcgcgga tcctgtcggc gtacgacttg tatccctcgg
     4681 cgggagcgtc catgacgtcg atcacctgcg cctcgtgcac gaacgggaga catgcgacgc
     4741 gcgcggccat cgagccgtcg cgcgccggaa ccgtcacgac gcctcccgcg ccgggggcga
     4801 gcggcttatg cacgacgtag acgccgatgc ccgcgaggag ggggccgagc acgcgcatga
     4861 gctcgggcga gtcgtggttg ccggggatca cgaccacccg ccggtcgttc gaggcgagcc
     4921 ggcgcagtga ctcgagcacg aggctcaggt aaggaagagg aggcgcggcc cggtcgaaga
     4981 ggtcgcccgc gaccagcacc gcgtcgacgt cctcgtcgtc cgcgatcgcg acgacctcgt
     5041 cgaggacggc ctcggcctcc gccgcgcggt cgatgcggcc gagcttcttg ccgctgtgcc
     5101 agtcgcccgt gtggaggatg cgcatccggc tacagcccct cgaacgggtc ggcgccggag
     5161 tcgtcgacct cttcgggccg cgtcgcccac ggcgggtacg ggaactcgag cacggtgggg
     5221 gccggtatcg acggctgcga cagcaccatc gtccccggct tgagcagccg cgcccgggcc
     5281 ctcgtcgacg ggagcatcca gccgtactcg gcccgttcgg cctcggccga gtcgagccgt
     5341 cccgagacct tgatcgacgc gttgccgagg atgtcggggt cgatgcgcga cgcgctctgc
     5401 tgcgcgccga tgagcaggat cccgagagac ctcccgcgct gcgcgacgtc gatcagcgtg
     5461 tccttgatcg gcgaccagcc ctcgcgcggc gcgtacttgt tgagctcgtc gagcacgatc
     5521 accgacagag ggaagcggcc gcggccctcc ttctcgcgat ggacctcggc gatcatcgcc
     5581 ccgacgacga accgctgggc ggcgtcgtgc aggtcgtgga tgtcgacgac cgtgactgcc
     5641 tcgcgggcgc ggtcgatccg gcgggaaggg ccggccttca cgagccgccc tacccggggc
     5701 agcgcgccgt agagccggcg catgaatgcg ctgacggtgc cctgtgcgac gcggctcgtc
     5761 cacgcgaggt cgggctcgcc gtcgtccggc tcgacgtagg tggctatctc ctcgacgagg
     5821 tcgccgaggg actcgatcac gcgctcgccg gggaagggca cgacgggctc gtccgagtgg
     5881 atcgcgtcgg tcgggtcgcg cagcacgatc gcaccgggcc gccccgcgac gtcgcaggcg
     5941 aaccgttcga gctgacgcgt gacccgctcg gcgacgaacg tgatctgctg gcgcgtgtcg
     6001 gccgcgtccg tgaacaagaa cgggagcagg tgctcgtcga cgaactcgcg cggggtccag
     6061 cggaacgcct cgacgccctc ggtccgcgcc cggctgtcgg ggatgccctc gctgcccgcg
     6121 tgcgacatgg ccggagccca gaacgagacc gagtcgaagg gggccgggtc catcccgagc
     6181 ttgcgccagt cgtccgcagc ctcctcggtg aagcggacgt tcggcttgtc gaggaacagc
     6241 aggtcctgcc ccttcacgtt gaacacgagg accttcaggt tgcgggaccc cttgccgagc
     6301 gacttcgggt gcgcggtcag gcagcgcagg aagaacaacg cgtacgaggt cttggtcgcg
     6361 acgcccgaca cgccggagat gctcatgtgc gcgcccttgg tgccgtcgaa gaagtcgacg
     6421 tcgacccaca cgggctcgtc gtcgcgagag agcccgaccg gcagagcgcg ggcggactcg
     6481 cccccgccca tctcgtcgac gtagagagcg cgctcgcgct cggcccccgt ggccttgaat
     6541 accggctggc cggagtcggg ggccacccac gtctcgggca gcacgcgcac ggtctggacg
     6601 gtcgcggtac gtgacttctc cgccggcaac gtgccctcga cggcgacgcg gaacgtgtcg
     6661 gactcgaacg acgcgccctc gtagacggcc gcggtctcga tcacgatccc gtaggtcgcg
     6721 acttccccca ccttcggaac gtgcgtgtag accacgacca ggtcgtcgac ctgcaggtac
     6781 tcgtcctcgt gcagcacgac gcggaactcc tgggtcgacg tgggttcgga gccgaggacg
     6841 agcccgacct cgcgatcggt catgccggca cctccgcacg cgcggccgcc gaccggatct
     6901 gccggtacac gagcgccggc tcgccgagca ggtggcgcag ccgcttctcg agcgctgcga
     6961 taggaacgag gttctgcggg gcccgcttgt cccagtacgg cttggacgcg tagcgcggca
     7021 acagcgccgt cgacgcgttc gcgagctcga tcgccgcctc gaccgagagc gccgccggga
     7081 cctcgcagcg cacgaggccc tgccacgcgt ggccgccggc atcccgctcg cagagccgga
     7141 cgtaccagga gtagcgcggg cgtttctcgc cgaacgagaa caacggcgtc cgctcgccgc
     7201 agccgagccg ggcgagcacg tcctcctcgg gagacttcag gtatcgcacc gagtgcgact
     7261 tgatgagccc gatcaccttg cgcttgccgg ggtccatctt cgcgagcggt ccgtcggcga
     7321 agacgaggtg gccctggtcg gccgcggcca ctgcgaggtt cgcctcgagc cggcgcatgt
     7381 gctcctgaag ggcctggaac aagacgtcgg tcgtgtcggc cggatgcggc atgggctcgt
     7441 aggagagggc cgggcccgcg tagagcccct gagcgtggcc gccgccgagc acgagccacc
     7501 gcgtgatcgt ctcggtcgtg atccgggccg cacacccgtc cggggacggc tcgcaaacga
     7561 ccgccccgac cccgatcgac ccggccaggc cgggcacggg catgtcgccg ccgttcatcc
     7621 cgaacagcct ggcgtcgacc cggcgggtcc cgtcgacgaa cgcgaccgtc tctaccccgg
     7681 gcgcgtcggg cgaccgcggc acgggctccc acggctcgcc ccgggtctcg tggtcggtcc
     7741 ggacgtcctc gacggagaag tcgtcgggcc ccccgaggtc gacctcggcg gcgtactcgt
     7801 ggctccacgc ctcgacggca atgcgcatgg agtcacacta aagagggggt gtgacaggtt
     7861 cccgggttcg ccccgaggtt ttttcccgtt tacggcgggc cctaccgaac cgccagggcc
     7921 gcctcgaccc ccgccacgac cttgtccacc atctcggccg tcgccgtggc ccccatcagg
     7981 ccgaccctga tcgccttgcc tgccgtcggg tccagcccgc ccgagatcat gatcccggag
     8041 ttcgccagca ggtgcttctg gatcgacgcg gggtcgtccg cccaggcagc gacgacgagg
     8101 ttcgcctcgg cccccgcctg cgggaccggt tcgagcccca gcgcccgcag cccctcccgg
     8161 cagcgcttgg ccaggtcggc acggcgctcg acccactcct ggacgccggc ctcgaggatc
     8221 ctccggacgc tcgcgtagag cgggagcagc aggttgctcg ggaaggtgac ggggtgcggg
     8281 tgccaggc
//
LOCUS       DASQDO010000463         5046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1028383, whole genome shotgun sequence.
ACCESSION   DASQDO010000463 DASQDO010000000
VERSION     DASQDO010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028383"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VEV43_12680"
     CDS             complement(<1..1100)
                     /locus_tag="VEV43_12680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYP24421.1"
                     /translation="MNDLERLLHERIERDGPLPFGAFMQLALYHPRLGYYSGGGERSG
                     WRGHFLTSPELDPAYGELWARGFEKAWAACGSPETFHVVEVGPGEGGFAHSVLSSVAG
                     AFGAALRYRLVERVPAVEERQRERLAAFDVTWSPSIAEVPRAEAGVVFANEVLDNLPV
                     HVVERRGGRILEVCVDSAGGRLAETLRPPAGPELEAWLARAGAEVPEGHRFEVTMAAE
                     SFVRRCAAAIGTGALVLVDYGAESGELAERARGTLVAYGEGGPSEDVLARPGEQDVTA
                     HANWTIVRAVCAELGWDVAGPLPQREVLLDLGARDLDGDLRESHRRALAEGRGAEAVR
                     ALSRRSALGALLDPGGLGGLGVVIATQGIEPGA"
     gene            complement(1097..1453)
                     /locus_tag="VEV43_12685"
     CDS             complement(1097..1453)
                     /locus_tag="VEV43_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24422.1"
                     /translation="MAEHDDNPNVDLVNRFLRGQNLEQVARVDEAIELYETILAARFD
                     SIGPYDRLIEIYSNRAAHREVVRVADAALESVHTYPEKRRWYEQMRDAAAKAASSVPK
                     AAPKAAPKRVGGAPEA"
     gene            1547..2434
                     /locus_tag="VEV43_12690"
     CDS             1547..2434
                     /locus_tag="VEV43_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HYP24423.1"
                     /translation="MNDEQLQKIRTGNGFIAALDQSGGSTPKALLKYGVAEDAYSNDD
                     EMFSLIHEMRSRIITSRAFTGDRVIGAILFENTMDRDIDGRNSVDYLWNEKNVVPFLK
                     VDKGLADESDGVQVMKPITNLDDLLGRAVDKGVFGTKMRSVVKLADKAGIDSIVEQQF
                     EIGRQIVARGLVPILEPEVDIKSPEKAQAEAMLRDGIFERLDGLGADEKVMFKLTLPE
                     EDNFYLKLTEHPNVVRVVALSGGYSREEANERLARNKGVVASFSRGLTEGLSADQSDQ
                     DFDAALDASIQSIYEASIA"
     gene            2542..4494
                     /gene="thrS"
                     /locus_tag="VEV43_12695"
     CDS             2542..4494
                     /gene="thrS"
                     /locus_tag="VEV43_12695"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HYP24424.1"
                     /translation="MSYVVTGDGAKDGFALAKEAAAPGPVALVVGGKERDLAFVVPSG
                     AEARVVVSTEDDGRYVIRHSTAHVLAQAVLRLYPGAKYSIGPPIEDGFYYDFDVETPF
                     TPDDLERIEAEMRSIVKENQRFVRREVERDEALALFAGQPYKVEIIEGVGSKGADDLT
                     QQEAAEGEVISVYENTAADASEPAFVDLCRGPHLPGTGRIKAFKLLRTAGAYWRGDEN
                     KPMLQRIYGTAWETKQALEDYLHRLEEAEKRDHRKLGRELELYSWEDEVGPGLALWHP
                     KGAIVRKTLEDLSREMHLERGYQPVFTPHLGKSTLWETSGHLGFYQENMFPRMELEGT
                     DYYAKPMTCAFHVLIYRSKTRSYRELPIRYSELGTVYRYERSGTLHGLMRARGLTQDD
                     SHIFCRPDQVVDEMVNVIDFFRALYGTVGLGPDNVRFSTKPDKAVGSQELWDLAEDAI
                     PKALEKAGLDYTVDEGDGAFYGPKIDIDVRDAIGRSWQVCTIQVDFNHPERFGLEYID
                     EHGERIRPVMIHRALFGSVERFTGVLVEHFAGAFPTWLAPLQAVLIPIADRHVAYART
                     VEERLRKAGIRAEVDDSTDTMGAKIRRQQLQKVPYMLVVGDNEAEGDTVSVRRRSGEE
                     TRGVAVDQLARDLAAEIAERRVEPSV"
     gene            4522..5001
                     /locus_tag="VEV43_12700"
     CDS             4522..5001
                     /locus_tag="VEV43_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012123544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HYP24425.1"
                     /translation="MQYVSTSGGDGGGCIFCEHLAKGDEVLYRGTTAFVLLNAFPYNT
                     GHLMVAPQRHAGELGDLSEEERHELMDLSAESVRIVREAMAAHGFNVGMNLGDVAGAG
                     IPGHLHMHVVPRWGGDTNFMTTVGETKVLPEMLADTAAKLKPLFDALGKGGLGRPPA"
BASE COUNT          839 a         1820 c         1697 g          690 t
ORIGIN      
        1 gcgcccggct cgatcccttg agtcgcgatc actacgccga ggccgcccag gcccccgggg
       61 tccaggagcg ccccgagggc gctccggcgc gagagcgctc gcacggcttc ggccccccga
      121 ccctcggcca gtgcccggcg gtgcgattcc cgtaggtccc cgtccaggtc ccgggcgccg
      181 agatccaaca gcacctcccg ctggggcagc ggcccggcta cgtcccaccc gagctcggcg
      241 cagaccgccc gcacgatcgt ccagttcgca tgggcggtca cgtcctgctc gccgggccgg
      301 gccaagacgt cctcgctcgg ccccccctcg ccgtacgcca ccagagttcc ccgggcccgt
      361 tccgccagct cccccgactc ggccccgtag tcgaccagga ccagagcgcc ggtcccgatc
      421 gcggcggcac aacggcggac gaacgactcc gcggccatcg tgacctcgaa gcgatggccc
      481 tccggcactt cggcccccgc ccgcgcgagc cacgcttcta gctccggccc cgccgggggc
      541 cgaagcgtct cggccagccg ccctcccgcc gagtcgacgc acacctcgag gatccgcccg
      601 ccccgccgct cgaccacgtg gaccggcagg ttgtcgagca cctcgttcgc gaacacgacg
      661 cccgcctccg cccgggggac ctccgcgatc gacgggctcc aggtgacgtc gaacgccgcg
      721 agccgctcac gctggcgctc ctccacggca ggcacgcgct cgacgagccg gtagcgcaac
      781 gccgctccga acgcaccggc aacgctcgac agcaccgagt gcgcgaaccc gccctcgccc
      841 ggccccacct cgaccacatg gaacgtctcc ggcgacccgc acgcggccca cgccttctcg
      901 aacccccgtg cccacagctc gccgtaggcg gggtcgagct ccggggacgt gaggaaatga
      961 cccctccacc cggaacgctc gccccctccg gagtagtagc ccagccgcgg gtggtagagc
     1021 gcgagctgca tgaaggctcc gaacggaagg ggcccgtctc gctcgatccg ctcgtggagc
     1081 aggcgttcga ggtcgttcaa gcctccggtg ccccacccac ccgcttaggg gccgccttcg
     1141 gggccgcctt cggcaccgac gaagcggcct tcgcagccgc gtcgcgcatc tgctcgtacc
     1201 agcgcctctt ctcgggatag gtgtggacgg actcgagggc cgcgtccgcg acccgcacga
     1261 cctcgcggtg ggcggcccgg ttcgaataga tctcgatcag ccggtcgtac ggcccgatgg
     1321 agtcgaaccg cgcggcgagg atcgtctcgt acagctcgat cgcctcgtcg acgcgggcga
     1381 cctgctcgag gttctgcccc cgcaggaagc gattgacgag gtcgacgttc gggttgtcgt
     1441 cgtgctcggc cacggctcaa tcgaaccaca acggcgcgaa tggccaggcg gcgaggtggt
     1501 gtgggattct cgggagaccg acttccactc acaggggtga tctgacttga acgacgagca
     1561 gctccagaag atccggaccg gcaacggctt catcgcggcc ctcgaccaga gcggcgggag
     1621 caccccgaag gcgctcctca agtacggcgt cgccgaggat gcctactcga acgacgacga
     1681 gatgttctcg ctgatccacg agatgcgcag ccgcatcatc acgagccggg ccttcaccgg
     1741 cgaccgcgtc atcggggcga tcctgttcga gaacacgatg gatcgcgaca tcgacggccg
     1801 caactccgtc gactacctgt ggaacgagaa gaacgtcgtc cccttcttga aggtcgacaa
     1861 gggcctcgcc gacgagagcg acggcgtcca ggtgatgaag ccgatcacga acctcgacga
     1921 cctcctcggc cgcgccgtcg acaagggcgt gttcggcacg aagatgcggt cggtcgtcaa
     1981 gctcgccgac aaggccggca tcgactcgat cgtcgagcag cagttcgaga tcggccgcca
     2041 gatcgtcgcc cgcggcctcg tcccgatcct cgagcccgag gtcgacatca agagccccga
     2101 gaaggcgcag gccgaggcga tgctccgcga cgggatcttc gagcggctgg acggcctcgg
     2161 cgccgacgag aaggtgatgt tcaagctgac gctcccggag gaggacaact tctacctgaa
     2221 gctcaccgag cacccgaacg tcgtgcgcgt cgtcgcgctg tccgggggct acagccgtga
     2281 ggaggcgaac gagaggctgg cgcgcaacaa gggcgtcgtg gcgagcttct cgcgcggcct
     2341 aaccgagggc ctctcggcgg accagagcga ccaggacttc gacgcggccc tcgacgcgtc
     2401 gatccagagc atctacgagg catccatcgc ctgacccccg gagcgacggg gccgagcagg
     2461 ctcgtcccct acccctcgct gttagtcaga caaatgtctg acaatttcgg agggcgccgg
     2521 gaagaccgtt agcatcgccc gatgagctat gtcgtcaccg gggacggggc caaggacggg
     2581 ttcgccctcg ccaaggaggc cgcggccccc ggccccgtcg cgctcgtcgt cggcggcaag
     2641 gagcgcgacc tcgccttcgt cgtgccctcc ggcgccgagg cccgcgtcgt cgtctcgacc
     2701 gaggacgacg gccgctacgt catcaggcac tcgaccgcgc acgtgctcgc gcaggccgtg
     2761 ctgcgcctct acccgggcgc caagtactcc atcggtcccc cgatcgagga cggcttctac
     2821 tacgacttcg acgtcgagac cccgttcacg cccgacgacc tcgagcggat cgaggccgag
     2881 atgcgctcga tcgtcaagga gaaccagcgc ttcgtccgca gagaggtgga gcgcgacgag
     2941 gcgctcgcgc tcttcgccgg ccagccctac aaggtcgaga tcatcgaggg cgtcggctcg
     3001 aagggcgcgg acgacctcac gcagcaggag gccgccgagg gcgaagtgat ctcggtctac
     3061 gagaacaccg ccgcagacgc gagcgagccc gcgttcgtcg acctgtgccg cggcccccac
     3121 ctccccggga ccggccggat caaggcgttc aagctcctgc gcaccgcggg ggcgtactgg
     3181 cgcggcgacg agaacaagcc gatgctccag cgcatctacg ggaccgcgtg ggagaccaag
     3241 caggcgctcg aggactacct ccaccgtctc gaagaggccg agaagcgcga ccaccgcaag
     3301 ctcgggcgcg agctcgagct ctactcctgg gaggacgagg tcggccccgg gctcgcgctg
     3361 tggcatccca agggcgcgat cgtccgcaag acgctcgaag acctgtcgcg ggagatgcac
     3421 ctggagcgcg ggtaccagcc cgtcttcacc ccgcacctcg gcaagtcgac gctgtgggag
     3481 accagcggac acctcggctt ctaccaggag aacatgttcc cgcgcatgga gttggaggga
     3541 accgactact acgcgaagcc tatgacctgc gcgttccacg tcctcatcta ccggtcgaag
     3601 acgcgctcgt accgcgagct cccgatcagg tactcggagc taggcaccgt ctaccggtac
     3661 gagcggtcgg gcacgctcca cgggctgatg agggcgcgcg ggctgacgca ggacgactcg
     3721 cacatcttct gccgtccgga ccaggtcgtc gacgagatgg tgaacgtcat cgacttcttc
     3781 agggcgctgt acgggaccgt cgggctcggc cccgacaacg tgcgcttctc gacgaagccg
     3841 gacaaggccg tcggctcgca ggagctgtgg gacctcgccg aggacgcgat ccccaaggcg
     3901 ctcgagaagg cgggcctcga ctacaccgtg gacgagggcg acggcgcgtt ctacgggccg
     3961 aagatcgata tcgacgtgcg cgacgccatc ggcaggtcgt ggcaggtctg cacgatccag
     4021 gtcgacttca accatccgga gcgcttcggc ctcgagtaca tcgacgagca cggcgagcgc
     4081 atccgcccgg tgatgatcca tcgcgcgctg ttcggctcgg tcgagcggtt cacgggcgtc
     4141 ctcgtcgagc acttcgccgg ggcgttcccg acgtggctcg cgccgctgca ggccgtgctg
     4201 atcccgatcg ccgaccgcca cgtcgcgtac gcgcgcacgg tggaggagcg gctgcgcaag
     4261 gccggcatcc gcgccgaggt cgacgactcg accgacacca tgggggccaa gatcaggcgc
     4321 cagcagctgc agaaggtccc ctacatgctc gtcgtcggcg acaacgaggc cgagggcgac
     4381 accgtgtcgg tgcggcgccg gagcggcgag gagacccgcg gggtcgccgt cgaccagctc
     4441 gcccgcgacc tcgcggccga gatcgccgag aggcgcgtcg agccgtccgt ctaggtggaa
     4501 cggctctgga gcccgtggag gatgcagtac gtgagcacct ccggcggtga cggcgggggc
     4561 tgcatcttct gcgagcacct cgcgaagggc gacgaggtcc tgtaccgcgg gacgacggcc
     4621 ttcgtgctgc tgaacgcgtt cccgtacaac accggccacc tgatggtcgc gccgcagcgc
     4681 cacgccggcg agctcggcga cctttcggag gaagagcgcc acgagctgat ggacctgtcc
     4741 gccgagtcgg tgcgaatcgt gcgcgaggcg atggccgcgc acggcttcaa cgtcgggatg
     4801 aacctcggcg acgtcgcggg ggccggcatc cccggccacc tgcacatgca cgtcgtgccc
     4861 cggtggggag gcgacacgaa cttcatgacg acggtggggg agaccaaggt gctgccggag
     4921 atgctcgcgg acacggcggc gaagctgaag cccctgttcg atgccctggg gaagggcggc
     4981 ctcggccggc ctcccgcatg agcgacccgt tccggctcga gaccgagcgc ctcgtcctgc
     5041 gcccgt
//
LOCUS       DASQDO010000464         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_538465, whole genome shotgun sequence.
ACCESSION   DASQDO010000464 DASQDO010000000
VERSION     DASQDO010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538465"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VEV43_12705"
     CDS             complement(<1..684)
                     /locus_tag="VEV43_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24426.1"
                     /translation="MSLLTARDDPLLAQWQYGLGRAVAWTSDAREAWAAPWIGTPEFG
                     TLTAQLVAWTLPPHDSEGIDVRFTPGQDGELDVEVTSFDDEGSPRNFYRTILRLVSPD
                     LEPTQTVLEQVGPGRYAGTLRAEDPGAYLVRVAQTFEDESGTDAASRTLGIVSPAAEE
                     YRRLGVDTPALEGYAAGGGGRVVPLDAEEGVREVWRHDIEASAFPTPIWPWLLLAAIL
                     LVPLDVGVRR"
     gene            complement(697..>1532)
                     /locus_tag="VEV43_12710"
     CDS             complement(697..>1532)
                     /locus_tag="VEV43_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYP24427.1"
                     /translation="ASLQVYVRDLGRGLVMLGGRDSYGAGGYIDTPIEETLPVYMTVR
                     DRERSPDVAMVAVVDRSGSMADCHCTGDSRDNANPSGTRGFEKVDIAKEAIIRAAEAL
                     APTDQLGVVTFDQNAHWAVRTAPIDFGALEDGLGFEANGTTNIYAGLKAAYDDLVNNP
                     ATLRHMILITDGWSQSGAYDDLLDDMRAAGITLSTIGTGGGSARILRELAEESGGRYY
                     DAADATTIPDIFLRETIRTAGEQIVEEEFQPVPSSASEILERLDAGRLPQLLGYNATT
                     A"
BASE COUNT          214 a          549 c          538 g          231 t
ORIGIN      
        1 ccgtcggaca ccgacgtcga gcggcaccag gaggatcgcc gcgaggagca gccatggcca
       61 gatcggggtc gggaaggcgc tcgcctcgat gtcgtgacgc cagacctcgc ggacgccctc
      121 ctcggcgtcg agcgggacga cgcgaccacc gccgccggcg gcgtatccct cgagcgcggg
      181 cgtgtcgacc ccgaggcggc gatactcctc ggcggccggt gacacgatcc cgagcgtgcg
      241 gctcgccgcg tcggtgccgc tctcgtcctc gaacgtctgc gcgacgcgaa ccaggtaggc
      301 gccgggatcc tccgcccgca acgtgcccgc gtaccgtccg ggaccaacct gctcgagcac
      361 ggtctgcgtc ggctcgaggt ccggcgacac gagcctgagg atcgtgcggt agaagttccg
      421 cggagacccc tcgtcgtcga acgaggtgac ctccacgtcc agctcaccgt cctgccccgg
      481 cgtgaaccgc acgtcgatcc cctcggaatc gtggggcggc agcgtccacg cgacgagctg
      541 cgcggtgagc gtcccgaatt cgggcgtgcc gatccacggc gcggcccacg cctcgcgcgc
      601 atcggacgtc caggcgaccg cccgtcccag cccgtactgc cactgcgcga ggagcgggtc
      661 gtcacgggcc gtgagcagcg acacggtcgc gcttccctac gcggtcgttg cgttgtaacc
      721 gaggagctgc gggagccgcc cggcgtcgag gcgctcgagg atctccgacg cggacgacgg
      781 gacgggctgg aactcctcct ccacgatctg ttcgcccgcg gtccggatcg tctcgcgcag
      841 gaagatgtcg gggatcgtcg ttgcgtccgc ggcgtcgtag tagcggccgc cggactcctc
      901 cgcgagctcc cggaggatcc gagcggagcc ccctccggtg ccgatggtgg acagcgtgat
      961 gccggccgcc cgcatgtcgt cgagcagatc gtcgtacgcg cccgactgcg accagccgtc
     1021 ggtgatgagg atcatgtgac ggagcgtcgc cgggttgttg acgaggtcgt cgtaggccgc
     1081 cttgaggccg gcgtagatgt tcgtggtccc gttcgcctcg aagcccaggc cgtcctccag
     1141 cgcaccgaag tcgatcggcg cggtgcggac ggcccagtgc gcgttctggt cgaaggtgac
     1201 gacgccgagc tggtcggtcg gcgccagcgc ctcggcggcg cggatgatcg cctccttggc
     1261 aatgtcgacc ttctcgaagc cgcgtgtccc ggacgggttc gcgttgtcgc ggctgtcacc
     1321 ggtgcagtgg cagtccgcca tgctccccga tcggtcgacg acggcgacca tcgcgacgtc
     1381 cggcgatcgc tcacgatcgc ggaccgtcat gtacaccggc agcgtctcct cgatgggcgt
     1441 gtcgatgtat ccgcccgccc cgtagctgtc ccgcccgccg agcatcacga ggccacgccc
     1501 gagatcgcgg acgtacacct ggagcgacgc ca
//
LOCUS       DASQDO010000465         8056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_661876, whole genome shotgun sequence.
ACCESSION   DASQDO010000465 DASQDO010000000
VERSION     DASQDO010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661876"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VEV43_12715"
     CDS             <1..895
                     /locus_tag="VEV43_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HYP24428.1"
                     /translation="DEELLALLDIPRQVLPQVVDSSGFVAEASLQGTLVPITGVAGDQ
                     QAALFGQACFEPGMAKNTYGTGSFVLMNTGERVASEHGLLASAAWDLGEGPRYVLEGS
                     IFVTGAALQWLRDGLGIIGAASEAGPLAESVPDTAGSYLVPAFTGLGAPVWDPYARGA
                     YVGITRGTTRAHLTRAAVEAMAYQTRDVVDAMAADSGTGLAELRVDGGASVMDFLCQF
                     QADQLGVVVRRAAMAETTALGAAYMAGLAAGVWDSPSDISERWSVDAEFEPRMSRDEA
                     DSLYEGWTKALERAKGWAEAD"
     gene            complement(892..1590)
                     /locus_tag="VEV43_12720"
     CDS             complement(892..1590)
                     /locus_tag="VEV43_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYP24429.1"
                     /translation="MLKALYRIRVEGLENVPKKGPAILAANHLSFLDSFFIPLVVKRR
                     KVTYLAKADYFKKWKTAWFFKSAGQISCERGGGDKSQQSLEIALDVLNEGKLLGIYPE
                     GTRSPDGYLYRGRTGVARLALASGVPVIPVGLVGTEKVMPKSAKIPRLWGRVEVVVRL
                     GEPIDFSRYAGKDTDRMALRSVTDEIVYEIMQLSGQEYRDEYASKTPSEPMPEPTRSI
                     YVDQIDLSEETLAG"
     gene            1782..2543
                     /locus_tag="VEV43_12725"
     CDS             1782..2543
                     /locus_tag="VEV43_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP24430.1"
                     /translation="MEFKVLEGAEEFELGEGPVGALLVHGFTGSPQSMRPLGDYLAER
                     GIAVMAPRLPGHGTAWQDLNVRKAEEWVQHVEDAFNVFSAEKEEVFLVGLSFGAALCL
                     NFAAHYPEKVDGIVALSTFLLTKDPRRFLSPIIRRVVSSIPGAGNDIADPDQRELCYD
                     RLPTGAAHVMLQFVKRARGNLAAIRSPILVMHSHNDHTAHPSAATLIHDSVSSEDKQL
                     VWVDDSYHVITLDRDRDEVYRRTYEFIKERSRHDL"
     gene            2533..3858
                     /locus_tag="VEV43_12730"
     CDS             2533..3858
                     /locus_tag="VEV43_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HYP24431.1"
                     /translation="MTSETVVLQGHIIDSLALPRTLDEIVALGAEYEIVELRLGVKAD
                     DESFARIKIRAADEHALGNVIERLRQHGANPETVEDAGVAEADVDGAFPPGFYSTTNL
                     MTDVRLGGRWIQVQRPEMDCGLVVEGDSVRTVAMSDVRAGMQIVMSGLGIRVHPPEKP
                     GGGDSQAFEFMSSDVSSEKPKALLVDRVAEQMREVKAQGKRIMWVAGPAVVHTGSGPD
                     LAALIRAGYVQAFFAGNGVAAHDIESNMFGTSLGVYLEQGTPADHGHEHHIRAINELR
                     RHGSFQAAIDAGVFGDGVVYHLVKSGADYLFGGSVRDDGPLPDVVTDMVEVQRQLRDI
                     IWGSGSPGRDAGTDGDQDLIGFCIVVGTMLHGIATGNCLPASVPLVCVDINQATVTKL
                     MDRGSFQSMGIITDVGLFIRELAERLAPDEIRPPQEEAEARVAGSRTRR"
     gene            3887..5815
                     /locus_tag="VEV43_12735"
     CDS             3887..5815
                     /locus_tag="VEV43_12735"
                     /inference="COORDINATES: protein motif:HMM:NF003780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HYP24432.1"
                     /translation="MRQIGRITLAAVVALSAVATAAGASPTPGRAQEPGRIVVRNGVT
                     QPVFSYADAVRETVYVQSSIDGDGDQQLDLLATDIIRPAATDGGDLQVPVIYEQSPYY
                     QSNGRGNEAEIKAEEDGDWVPTVFPLYYDNYFVPRGYAVVLQDMPGTRNSEGCMVLGG
                     EAEELAAKETIDWLNGRGKAFTATGEEVTADWTTGRVGMIGKSYDASVANGAATTGVE
                     GLETIVPIGGIDRWYDYHLNNGVQYVNAYTTPGLFSFVIDQPPGDDEERGKDWVENTF
                     TENSTCSAKGGEIVGKAADPQGDYNEFWDERDYLKDVDKVEASVFVVHGMNDWNVKPN
                     NYVQWWQGLQRHDVPRKIWLSQTGHVDPFDFRRGEWVRTLHRWYDYWLHGIDNGIMDE
                     PIADVEVAPNRWKKFTSWPDRRARKVKLFLAPAKKKRPGTFSLRRPKRAVMVFQDDPE
                     QSEDDMVASPAEEGANRLIFLTKKLRRSARVSGTVKVDIRAMVDQVDTNLTALLVDYG
                     KTKRVSSSSDGVTTLDKESCHGQSTEADDSCYFLVERIIDQDPVEIVSRGWLDARHRD
                     SLRQGEPMVPGELYRFRWEIFGDDYTFKKGHRLAIVIAGSDPDYTIPDQTQANVNVRI
                     RRSKVVIPVVGGKRRLRL"
     gene            complement(5819..7156)
                     /locus_tag="VEV43_12740"
     CDS             complement(5819..7156)
                     /locus_tag="VEV43_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HYP24433.1"
                     /translation="MGRGEGSRRRVLIVGAAGRDFHNFNVAYRDDERFEVVAFTATQI
                     PGIEGRVYPSVLAGGLYPDGIPIFPEGQLETLVAEHDVDEVVFSYSDVSHEAVMHIGS
                     RALAAGADYTLLGPRSTMIASNKPVVAVCAVRTGSGKSQTTRAVSRILKEAGKQVAVV
                     RHPMPYGRLQDQRAQRFETYDDLLAADCTIEEREEYEPHLEQGSIVFAGVDYGEILAM
                     AERDADVVVWDGGNNDLPFYEPDVHVTVADPLRAGHETSYHPGEANLRMADVVVINKV
                     DSASEEDLAALEKTIETVNPGAAVVHGRSPITVPNGEMIEGKKVLVVEDGPTLTHGEM
                     TFGAGVVAARRAGAASLVDPRPWAAGSIKGVYEAYPHIGELLPAMGYSEEQRRDLAAT
                     INASDADIVLVATPIDLRKICDIDKPALRATYELDDSASKPTLAEELHARLSL"
     gene            7200..7799
                     /locus_tag="VEV43_12745"
     CDS             7200..7799
                     /locus_tag="VEV43_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYP24434.1"
                     /translation="MEDRKLTKRGSKRRVQLLESASRQFAEQGYHGTTVGDICDSLGV
                     GKGVFYWYFESKEALFSEILQESLLELRRAQERAIATVDDPVARIEQGIRASIDFFRA
                     SPGFLSLIRTAARYEEFSIFVEKGQQIIAADTAAHIKDGMSRGVIRHGDPDLMAHGIL
                     GAIFHFVEVYFGTAAEATADRPQLADEAVTFCLKGLLER"
     gene            complement(7796..>8056)
                     /locus_tag="VEV43_12750"
     CDS             complement(7796..>8056)
                     /locus_tag="VEV43_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYP24435.1"
                     /translation="RIGWEITRPPATMFAWARIPEEFRPMGSLEFAKHLVREAKVAVS
                     PGVGFGASADEYVRFALVENEQRIRQAVRGLRKALEQGPPAA"
BASE COUNT         1306 a         2728 c         2804 g         1218 t
ORIGIN      
        1 ggacgaagag ttgctggcac ttctggatat cccccgacaa gtacttcccc aggtggtgga
       61 ttcaagcggt tttgttgcag aagcttcgct gcagggcacg ctggttccta ttaccggcgt
      121 ggccggcgac cagcaggccg ctctgttcgg ccaggcctgc ttcgagcccg gcatggccaa
      181 gaacacctac ggcacgggct cgttcgtcct gatgaacacc ggcgagcggg tggcctcgga
      241 gcacgggctg ctggcgagcg cggcgtggga cctcggcgag gggccgcgct acgtcctcga
      301 gggcagcatc ttcgtcacgg gggccgcgct ccagtggctg cgagacgggc tcgggatcat
      361 cggggccgcg tccgaggcgg gccccctggc cgagtccgtc cccgacaccg ccggcagcta
      421 cctggtcccg gcgttcaccg ggctcggcgc cccggtctgg gacccctacg cccggggtgc
      481 ctacgtcggg atcacgcggg ggacgacacg agcgcacctg actagggccg ccgtcgaggc
      541 gatggcgtac cagacgcggg acgtcgtcga cgcgatggcg gcggactcgg gcaccgggct
      601 cgccgagctg cgggtcgacg gaggcgcgag cgtcatggac ttcctctgcc agttccaggc
      661 cgaccagctc ggggtcgtcg tgcgccgggc ggcgatggcc gagacgacgg cactgggggc
      721 cgcttacatg gcggggctgg ccgccggggt gtgggactcg ccctccgaca tctcggagag
      781 gtggtccgtc gacgccgagt tcgagccccg catgtcccgc gacgaggccg attcgctgta
      841 cgaggggtgg acgaaggcgc tggaacgggc gaagggctgg gccgaggccg attagccggc
      901 gagagtctct tccgacaggt cgatctggtc gacgtagatc gagcgggtcg gctcgggcat
      961 cggctcggac ggcgtcttcg acgcgtactc gtcccggtac tcctggcccg aaagctgcat
     1021 gatctcgtac acgatctcgt cggtcaccga gcgcagagcc atgcggtccg tgtccttgcc
     1081 ggcgtagcgg gagaagtcga tcggctcgcc gaggcggacg acgacttcga cccggcccca
     1141 cagccgcggg atcttggcgg acttcggcat gaccttctcg gtcccgacga gcccgaccgg
     1201 gatcacgggg acacccgacg ctagggcgag ccgggcgacg ccggtcctgc ccctgtagag
     1261 atagccgtcg ggcgagcggg tcccctcggg gtagatgccg aggagcttcc cctcgttcag
     1321 cacgtcgagc gcgatctcga gcgactgctg ggacttgtcg ccgccgccgc gctcgcacga
     1381 gatctggccg gcgctcttga agaaccacgc ggtcttccac ttcttgaagt agtcggcctt
     1441 cgcgaggtac gtgaccttgc gccgcttgac gacgagcggg atgaagaacg agtcgaggaa
     1501 cgagaggtgg ttcgcggcga ggatcgcggg gcccttcttg ggcacgttct cgaggccctc
     1561 tacccggatc cggtagagcg ccttcagcaa cggtttgaga atggctttga agacccagta
     1621 catgagttcc ggtaagcccc tcttcttgat gcccccgcat ccgactctac ccaggcggtc
     1681 aaggcagaag tcttgacctc acggtcaaga cagcgcgcgg ctgtagcgtt ggacgccgtt
     1741 agcttgtccg ccgttacccg gagcctagga ggatgccgcc cgtggagttc aaggtgctgg
     1801 agggggccga ggagttcgag ctcggcgaag gtcccgtcgg tgcgctgctc gtccacggct
     1861 tcaccggtag cccgcagtcg atgcggcccc tcggcgacta cctcgcggag cgcgggatcg
     1921 ccgtgatggc gccgcgcctg ccgggtcacg ggaccgcgtg gcaggacctc aacgtgcgca
     1981 aggccgagga atgggtccag cacgtggagg acgcgttcaa cgtcttctcg gccgagaagg
     2041 aggaggtctt cctcgtcggg ctctcgttcg gcgcggcgct ctgtctgaac ttcgccgcgc
     2101 actatcccga gaaggtcgac gggatcgttg cgctgtcgac gttcctgctc acgaaggacc
     2161 cccggaggtt cctctcgccg atcatccggc gcgtcgtctc ttcgatcccc ggcgcgggca
     2221 acgacatcgc cgaccccgac cagcgtgagc tctgctacga caggctcccc accggcgctg
     2281 cgcacgtgat gctgcagttc gtgaagcggg cgcgcggcaa cctcgccgcg atccgttcgc
     2341 cgatcctcgt gatgcactcg cacaacgacc acacggcgca cccgagtgcg gcgacgctga
     2401 tccacgactc ggtgtcgtcg gaggacaagc agctcgtgtg ggtcgacgac tcgtaccacg
     2461 tgatcaccct cgaccgcgac cgcgacgagg tctaccggag gacctacgag ttcataaagg
     2521 agcgctcgcg ccatgacctc tgagaccgtc gtcctccagg gacacatcat cgactcgctc
     2581 gcgctcccgc gcacgctgga cgagatcgtg gcgctggggg ccgagtacga gatcgtcgag
     2641 ctgaggctcg gcgtcaaggc cgacgacgag tcgttcgcgc gcatcaagat cagggccgcg
     2701 gacgagcacg cactcggaaa cgtgatcgag cggctccgcc agcacggcgc gaacccggag
     2761 acggtcgagg acgcgggcgt agcggaggcc gacgtcgacg gggcgttccc tccgggcttc
     2821 tactccacga ccaacctgat gaccgacgtg cggctcggcg gccgctggat acaggtgcag
     2881 aggcccgaga tggactgcgg cctcgtcgtc gaaggcgact ccgtgcgcac cgtcgcgatg
     2941 agcgacgtgc gggccggcat gcagatcgtg atgagcgggc tcgggatccg cgtgcacccg
     3001 ccggagaagc ccggcggcgg cgactcgcag gcgttcgagt tcatgtcgtc cgacgtctcg
     3061 agcgagaagc cgaaggctct gctcgtcgac cgcgtcgccg agcagatgcg agaggtcaag
     3121 gcgcagggca agcgcatcat gtgggtcgcc ggccccgcgg tagtgcacac gggctcgggg
     3181 ccggacctcg ccgccctgat ccgcgccgga tacgtccagg cgttcttcgc gggcaacggc
     3241 gtcgccgcgc acgacatcga gtcgaacatg ttcgggacgt cgctcggggt gtacctcgaa
     3301 cagggcacgc ccgccgacca cggccacgag caccacatcc gcgcgatcaa cgagctgcgc
     3361 cggcacggct cgttccaggc cgcgatcgac gcgggcgtct tcggggacgg cgtcgtctac
     3421 cacctcgtga agagcggggc cgactacctc ttcggcggga gcgtgcgcga cgacggtccc
     3481 ctgcccgacg tcgtcaccga catggtcgag gtccagcggc agctgcgcga catcatttgg
     3541 ggaagcgggt cgcctgggcg agacgccgga acggatggcg accaggacct gatcgggttc
     3601 tgcatcgtcg tcggcacgat gctgcacggg atcgcgacgg gcaactgcct gccggcctcg
     3661 gtcccgctcg tgtgcgtcga catcaaccag gcgaccgtga cgaagctgat ggaccgcggc
     3721 tcgttccagt cgatggggat catcacggac gtgggcctgt tcatccggga gctggccgag
     3781 cgcctggcgc cggacgagat ccggcccccg caggaggaag ccgaggcccg ggtagcgggc
     3841 tcccgtacga ggaggtagag cggcccccgc gccgaacccc tgaggcatga gacaaatcgg
     3901 acggatcacc ctcgccgcag tcgttgccct ctcggccgtt gccacggccg ccggggcctc
     3961 ccccacgccc ggccgggccc aggagccggg gcgcatcgtc gtccgtaacg gcgtcaccca
     4021 gcccgtcttc tcgtacgcgg acgccgtgcg cgagaccgtc tacgtgcagt cgagcatcga
     4081 cggcgacggc gaccagcagc tcgacctgct cgccaccgac atcatccggc cggccgccac
     4141 cgacggcggc gacctccagg tccccgtgat ctacgagcag agcccctact accagagcaa
     4201 cgggcgcggc aacgaggccg agatcaaggc cgaggaggac ggggactggg tcccgacggt
     4261 cttcccgctc tactacgaca actacttcgt cccgcgcggc tacgcggtcg tgctgcagga
     4321 catgcccggc acgcgcaact ccgagggctg catggtcctc ggcggcgagg ccgaggagct
     4381 cgcggcgaag gagacgatcg actggctcaa cggccgcggc aaggccttca ccgcgacggg
     4441 cgaggaggtc accgccgact ggacgaccgg ccgcgtcggg atgatcggca agtcctacga
     4501 cgccagcgtc gcgaacggag cggctacgac cggcgtggaa ggcctcgaga ccatcgtgcc
     4561 tatcggcggg atcgaccgct ggtacgacta ccacctcaac aacggcgtcc agtacgtcaa
     4621 cgcgtacacg acgccggggc tgttcagctt cgtcatcgac cagccgcccg gcgacgacga
     4681 ggagcgcggc aaggactggg tcgagaacac gttcaccgag aactcgacgt gcagcgccaa
     4741 gggtggcgag atcgtcggga aggccgccga tccccagggc gactacaacg agttctggga
     4801 cgagcgcgac tacctcaagg acgtcgacaa ggtggaggcg agcgtgttcg tcgtccacgg
     4861 catgaacgac tggaacgtca agccgaacaa ttacgtccag tggtggcagg ggctacagcg
     4921 ccacgacgtg ccgcgcaaga tctggctctc ccagacgggg cacgtcgacc cgttcgactt
     4981 ccggcggggc gagtgggtcc ggacgctcca ccgctggtac gactactggc tgcacgggat
     5041 cgacaacggg atcatggacg agccgatcgc cgacgtcgag gtcgccccga accgatggaa
     5101 gaagttcacg tcgtggcccg accgacgcgc ccgcaaggtc aagctgttcc tcgccccggc
     5161 gaagaagaag cggcccggga ccttcagcct caggcggccc aagcgtgcgg tcatggtctt
     5221 ccaggacgac cccgagcagt cggaggacga catggtcgcc tcgccggcgg aagagggcgc
     5281 caaccggctg atcttcctca cgaagaagct ccggcgcagc gcgcgcgtga gcgggacggt
     5341 gaaggtcgac atccgcgcga tggtcgacca ggtcgacacg aacctgaccg cgcttctggt
     5401 cgactacggc aagacgaagc gcgtctcgag cagcagcgac ggcgtcacga cgctcgacaa
     5461 ggagtcgtgc cacgggcaga gcaccgaggc cgacgactct tgctacttcc tggtcgagag
     5521 gatcatcgac caggacccgg tcgagatcgt ctcgcgcggg tggctcgacg cgcggcatcg
     5581 cgattcgctg cgacagggcg agccgatggt ccccggcgag ctctaccgct tccgctggga
     5641 gatcttcggc gacgactaca cgttcaagaa gggtcaccgc ctggcgatag tgatcgcggg
     5701 aagcgacccc gactacacga tccccgacca gacgcaagcg aacgtgaacg tccgcatccg
     5761 ccggagcaag gtggtcatcc cggtggtcgg cgggaaacgg cgcctgcgcc tctagcggct
     5821 acaggctcaa gcgggcgtgt agctcttcgg ccagggtcgg cttcgacgcc gagtcgtcca
     5881 gctcgtacgt cgcgcgcagt gccggcttgt ctatgtcgca gatcttgcgc aggtcgatcg
     5941 gcgtcgccac gaggactatg tccgcgtccg acgcgttgat cgtcgccgcg aggtcacggc
     6001 gctgctcttc ggagtagccc atcgcaggca gcagctcgcc tatgtgcggg taggcctcgt
     6061 acacgccctt gatcgagccc gcggcccacg gccgggggtc gaccagtgac gccgctccgg
     6121 cgcgccgggc cgcgaccacc ccggccccga acgtcatctc gccgtgtgtg agcgtcggcc
     6181 cgtcctcgac cacgaggacc ttcttgccct cgatcatctc gccgttcggc accgtgatcg
     6241 gcgagcggcc gtggacgacc gcagcgccgg ggttgacggt ctcgatcgtc ttctcgagcg
     6301 cggcgaggtc ctcctccgac gcggagtcga ccttgttgat cacgacgacg tcggccatgc
     6361 ggaggttggc ctcgcccggg tggtacgagg tctcgtgccc ggcccggagc gggtcggcga
     6421 cggtcacgtg cacgtccggc tcgtagaacg gcaggtcgtt gttgcccccg tcccacacga
     6481 cgacgtcggc gtcgcgctcg gccatggcga ggatctcgcc gtagtcgacc cccgcgaaca
     6541 cgatcgagcc ctgctcgagg tgcggctcgt actcctcacg ctcctcgatc gtgcagtcgg
     6601 cggcgagcag gtcgtcgtag gtctcgaagc gctgcgcgcg ctggtcctgg aggcgtccgt
     6661 agggcatcgg gtgccggacg acggcgacct gcttgcccgc ttccttgagg atgcgcgaga
     6721 ccgcgcgggt cgtctgcgac ttgcccgacc ccgtgcgcac cgcgcacacc gcgacgaccg
     6781 gcttgttcga cgcgatcatc gtcgagcggg ggccgagcaa cgtgtagtcc gcgccggctg
     6841 cgagagcgcg cgagccgatg tgcatcacgg cctcgtgcga cacgtcggag tagctgaaca
     6901 ccacctcgtc gacgtcgtgc tcggcgacga gcgtctcgag ctggccttcc gggaagatcg
     6961 ggatgccgtc gggatagagg ccgccggcga gcaccgacgg gtacacgcgg ccctcgatcc
     7021 ccgggatctg cgtggcggtg aaggcgacga cctcgaagcg ctcgtcgtcg cggtaggcga
     7081 cgttgaagtt gtggaagtcg cggcccgcgg cccccactat caggaccctg cgacgcgacc
     7141 cctctccacg acccacgagg cggcagccta tacctcgcct aggaactagg gtgtcgccga
     7201 tggaggacag gaagctcacg aaacggggct ccaagcggcg ggtccagctc ctcgaatcgg
     7261 cctcgcgcca gttcgccgaa cagggctacc acggcacgac ggtcggcgac atctgcgact
     7321 ccctcggcgt cggcaagggc gtcttctact ggtacttcga gtcgaaggaa gcgctgttct
     7381 cggagatcct ccaggagtcc ctgctcgagc tccggcgggc ccaggagcgg gcgatcgcga
     7441 ccgtcgacga tcccgtcgcg cggatcgagc agggcatccg ggcgtcgatc gacttcttcc
     7501 gggcctcgcc cggcttcctc tcgctgatcc gcaccgcggc ccgctacgag gagttctcga
     7561 tcttcgtcga aaagggccag cagatcatcg cggccgacac cgcggcgcac atcaaggacg
     7621 ggatgtcccg cggcgtgatc cgccacggcg accccgacct gatggcgcac gggatcctcg
     7681 gggcgatctt ccacttcgtc gaggtgtact tcgggacggc ggcggaagcg acggcggacc
     7741 ggccgcagct ggccgacgag gccgtgacgt tctgcctgaa gggcttgctg gagcgctagg
     7801 ccgccggggg gccctgctcc agggccttgc gcaggcctcg cacggcctgg cggatgcgct
     7861 gctcgttctc gaccagcgcg aagcgcacgt attcgtccgc gctggcaccg aagccgacgc
     7921 ccggtgacac cgcgaccttc gcctcgcgta ccaggtgctt cgcgaactcg agcgagccca
     7981 tgggccggaa ctcctcgggg atgcgcgccc aggcgaacat cgtcgccggg ggccgcgtga
     8041 tctcccaccc gatgcg
//
LOCUS       DASQDO010000466         5180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_111401, whole genome shotgun sequence.
ACCESSION   DASQDO010000466 DASQDO010000000
VERSION     DASQDO010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111401"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3072
                     /locus_tag="VEV43_12755"
     CDS             <1..3072
                     /locus_tag="VEV43_12755"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP24436.1"
                     /translation="TAPGRTDDSFTFQVIDRGDPDGCGLPVPGTCTLPLDSNDATINV
                     SFDNAAPAAAPGDATTPEDVAVTIQLAATDADGDDLTYSVASLPAHGDLYPGNDPSGT
                     ALTSLPAVLPGTRVTYLPDSDYNGDDSFTFTAWDGSAASTPATVDLTVTPVNDAPEAS
                     DGSIDVAGGSVTGSVNLATLADDTETPDAGLTYEVVSGPDDGDVTLNGSTLTYTRTAP
                     GRTADELTFAVTDRGDPDDCGAANAACAAARTSTPATIAVTFGNAAPTAAPGVTTTPE
                     DTPVAVDLDGNDADGDALAYVVTSLPSDGTLRSGGNDVTTVPFELPGRTVTYVPDDDF
                     HGSDSFGFETSDGRLDSDGATVSVTVTPVNDRPAADHGAMTVTAAQTTAQLDLATLAR
                     DVETDDADLTYEIVSQPADGTATLNGSTVTYTRTAPGRDDDELTFKVTDRGDPDDCDA
                     PGAGCAAARDSVVATVSVAFDYTRPTAAGQTLQTPEDTPLTVALTGTSPSDPGSRFEV
                     TSLPEHGSLLQGQTELGTVPFELAGAGVVYAPDPHFSGEDSFTFVKTGLQDSAPAEIA
                     VTVTPVNDVPVARDAAADAPPATTTTLTLEATDVDHPALELLLVDAPRNGTLGPLTVQ
                     CTPAGAGTSCAATADYTPAAGYDGPDGFTFVADDGTDVSPKATVSLNVPAAPAPPPPP
                     DGGGNPVPQPTPTVSPTPAPSATPTPTATPTREPDPTPKPTPAPTPTPDGEKPCGQPI
                     EVEANGEPIVGTPCGELITVHADVPATIEALGGDDTIFVFGSGEVHVSAGGGDDQVSC
                     LESTATVLGGKGKDAIECGAGDDVLRGGPGRDAIAAGDGDDRVSGGNGGDAIDGSSGD
                     DVLVGGRGKDDLYGGGGDDLLKGQAKPDLIRAGAGADLLRGAGGDDVEYGGKGADKVQ
                     AGPGDDRLWGGRGADRLQGNEGNDHIQGGGGDDFVRGRAGDDGLGGRGGDVVLRGGPG
                     DDVLRGVGGDDELQSGSGRNDVDGGLGVDLCVTGTKDHVWSGCERHLKRRF"
     gene            3084..3581
                     /locus_tag="VEV43_12760"
     CDS             3084..3581
                     /locus_tag="VEV43_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HYP24437.1"
                     /translation="MPAARPPVQRVHPPRRLMKAINPIVRWRLRRRARGETSRRLMVL
                     HVTGRRTGAPYRVPVGRQTVDGRLCAFTDGSWRHNLRGGADVEVTIDGKTKSARAELE
                     EDPGRIARVFLDRIEEVGVAEASRALGIRVNVARSPTLHELRDAILRSGLSIVYVDVD
                     EDGRS"
     gene            complement(3578..4036)
                     /locus_tag="VEV43_12765"
     CDS             complement(3578..4036)
                     /locus_tag="VEV43_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24438.1"
                     /translation="MLVAAAVVASLSSACSTEAEGEPLASCSLPPSIGSPSGLPDDFP
                     QLEGVDLTEFQLKKQFVSTEGFDTRSVEELFDAFRPVLEDEGFDVVNSDFEGFEAELY
                     FAKANSLAGIVSLREGPCDGYVKVNVIYDPLETEAGRKAVKKTRRLSGEG"
     gene            complement(4124..>5180)
                     /locus_tag="VEV43_12770"
     CDS             complement(4124..>5180)
                     /locus_tag="VEV43_12770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYP24439.1"
                     /translation="NSGHFLHRSTDGGQTFTAGVDDGAGLGDLQIDRRNGTLYEAYYD
                     GGVLSMTAYRKARKGNLEDRDTHEVASGVSMLSHWPSFDIDSGGNHYLTWDESGQGDR
                     GPGIWFSYSEDGGRTWKAPTRVDKNANTDIWPWLAVGDEGKVAVAWLGANEELPNHDA
                     ETQGDQEWRVYVAQTLNGLGCKGASSPGFKVATATPRAVHTSTICQGGTTCQAELVDR
                     RLGDFFTIEIDNSGHVWGGYSDTAEGGATALPGFVHQSGGIPFIAQPSRGGNDGNQGG
                     NDGGDPPGGGGGGGDEDESNGGGGDDATDDGDDAAATPAERSSLAATGATILKFVALG
                     AALIALGLFVKRRAAKA"
BASE COUNT          761 a         1931 c         1799 g          689 t
ORIGIN      
        1 acggcccccg gccgcaccga cgactccttc acgttccagg tgatcgaccg cggcgacccc
       61 gacggctgcg gcctgccggt ccccggcacg tgcacgctcc cgctcgacag caacgacgca
      121 acgatcaacg tctcgttcga caacgcggcg ccggccgcgg cccccggcga cgcgacgacg
      181 cccgaggacg tggccgtcac gatccagctc gcggcgacgg acgccgacgg cgacgacctc
      241 acgtactcgg tggcctcgct gccggcgcac ggcgacctct acccgggcaa cgatcccagc
      301 ggcaccgcgc tgacgtcgct tccggccgtc ctgccgggga cgagggtcac gtacctcccg
      361 gactccgact acaacggcga cgactccttc acgttcacgg cgtgggacgg ctccgccgcc
      421 tcgacgccgg caaccgtcga cctgacggtc acgccggtca acgacgctcc cgaggcctcc
      481 gacggctcga tcgacgtggc cggtggatcc gtcacgggct cggtgaacct cgcgacgctc
      541 gccgacgaca ccgagacccc ggacgcgggc ctcacctacg aggtcgtctc cgggccggac
      601 gacggagacg tgacgctgaa cggctcgacc ctcacctaca cccgcaccgc gccgggacgt
      661 accgccgacg agctgacgtt cgcggtgacc gaccgcggcg accccgacga ctgcggtgcg
      721 gcgaacgcgg cgtgtgccgc ggctcgcacc agcacccctg ccacgatcgc ggtcacgttc
      781 ggcaacgccg ctccaaccgc ggcccccggc gtgacgacca cgcccgagga cactccggtc
      841 gcggtcgacc tggacgggaa cgacgccgac ggggacgctc tggcgtacgt cgtcacctcg
      901 ctcccgtccg acgggaccct gcgctccggc ggcaacgacg tcacgacggt tccgttcgag
      961 ctccccggcc gcacggtgac gtacgtgccg gacgacgact tccacggcag tgactcgttc
     1021 gggttcgaga cgtccgacgg ccgcctcgac tcggacggcg cgacggtgtc ggtcacggtt
     1081 accccggtga acgaccgtcc ggccgcggac cacggtgcga tgaccgtgac cgccgcgcag
     1141 acgactgcac agctcgacct cgcgacgctc gcccgggacg tcgagaccga cgacgccgac
     1201 ctcacctacg agatcgtgtc gcagccggcc gacggcaccg cgacgctcaa cgggtcgacc
     1261 gtgacctaca cgcggaccgc acccgggcgc gacgacgacg agctcacgtt caaggtcacc
     1321 gaccgcggcg accccgacga ctgcgacgct cccggcgcgg gctgcgcggc ggcgcgcgac
     1381 agcgtcgtgg ccaccgtttc cgtggcgttc gactacacgc ggccgacggc cgcgggccag
     1441 acgctgcaga cccccgagga cactccgctc acggtggcgc tgacgggcac gtcgcccagc
     1501 gaccccgggt cgcggttcga ggtcacctcg ctgcccgagc acggctcgct gctgcaaggt
     1561 cagacggagc tcggcaccgt gccgttcgag ctcgccggcg cgggcgtcgt gtacgcgccc
     1621 gacccgcact tctccggaga ggactccttc acgttcgtga agaccgggct gcaggactcg
     1681 gcccccgcag agatcgcggt gacggtcacg ccggtcaacg acgtcccggt ggctcgcgac
     1741 gcggcagccg acgctcctcc ggcgactact acgacgctca cgctcgaggc gaccgacgtc
     1801 gaccaccccg cgctcgagct cttgctcgtc gacgcaccgc gcaacggaac gctgggaccg
     1861 ctgaccgtgc agtgcacgcc ggccggcgcg ggcacgtcct gcgccgcgac cgcggactac
     1921 acgccggccg ccggttacga cggccccgac ggattcacgt tcgtggccga cgacggcacc
     1981 gacgtctcgc cgaaggcgac ggtctcgctg aacgtccccg cggcccccgc tcccccgcct
     2041 ccgcccgacg gcggcgggaa cccggtgccg cagccgactc cgaccgtgtc gcccacgcct
     2101 gctccgagcg caactccgac ccccactgcg acaccgacgc gggagcccga cccgaccccg
     2161 aagccgacgc cggctccgac gcccactccc gacggcgaga agccgtgcgg gcagcccatc
     2221 gaggtcgagg cgaacggcga gccgatcgtc ggcacgccct gcggtgagct catcaccgtg
     2281 cacgcagacg tgcccgcgac catcgaggcg ctcggcggcg acgacacgat cttcgtgttc
     2341 ggctccggcg aggtccacgt ctcggccggc ggcggcgacg accaggtctc gtgcctcgag
     2401 tcgacggcga cggtgctcgg cggcaagggc aaggacgcca tcgagtgcgg cgccggggac
     2461 gacgtgctgc gcggcgggcc gggccgggac gcgatcgcgg ccggtgacgg cgacgaccgc
     2521 gtctccggcg gcaacggcgg ggacgccatc gacgggagct ccggcgacga cgtgctcgtc
     2581 gggggccgcg gcaaggacga cctgtacggc ggtggtggcg acgacctgct caagggccag
     2641 gcgaagcccg acctgatccg cgccggcgcg ggagccgacc tgctccgcgg cgccggtggc
     2701 gacgacgtcg agtacggcgg caagggtgcc gacaaggtcc aggccggccc gggcgacgac
     2761 cgcctgtggg gcggccgcgg cgccgacagg ctccagggca acgaaggcaa cgaccacatc
     2821 cagggcggtg gcggcgacga cttcgtgcgg ggccgcgccg gcgacgacgg gctcggggga
     2881 cgcggtggcg acgtcgtgct ccgcggcggc cccggcgacg acgtgctgcg cggcgtcggc
     2941 ggtgacgacg agctccagtc cggctcgggg cgcaacgacg tggacggggg cctgggcgtg
     3001 gacctgtgcg tgaccggtac gaaggaccac gtctggagcg gctgcgaacg acacctgaag
     3061 cggaggttct agggtcgcgg gccatgcccg cggcccggcc ccccgtccag cgcgtccatc
     3121 cgccgcgccg gctcatgaag gcgatcaacc cgatcgtccg gtggcggctg cgccggcgtg
     3181 cccgcggcga gacctcccgc aggctcatgg tcctgcacgt caccgggcgt aggaccgggg
     3241 ccccataccg cgtgccggtc ggccgtcaga cggtggacgg gcggctgtgc gccttcaccg
     3301 acggatcgtg gaggcacaac ctgcgcggcg gagcggacgt cgaggtcacg atcgacggga
     3361 agacgaagag cgctcgtgcc gagctcgaag aggacccggg ccggatagcg cgcgtgttcc
     3421 tggaccggat cgaagaggtg ggagtcgcgg aggcttcacg cgcgctcggc atccgggtca
     3481 acgtcgcgcg gagcccgacg ctgcacgagc tgcgcgacgc gatcctccgc tcgggcctgt
     3541 cgatcgtgta cgtcgacgtc gacgaggacg ggcgctccta gccctcgccc gacagacgcc
     3601 tcgtcttctt cacggccttg cgcccggcct cggtctcgag cgggtcgtag atgacgttca
     3661 ccttcacgta gccgtcgcac gggccctcgc gcagcgagac gatgccggcc aggctgttcg
     3721 ccttcgcgaa gtagagctcg gcctcgaacc cttcgaagtc cgagttgacg acgtcgaagc
     3781 cctcgtcttc caagacggga cggaacgcgt cgaagagctc ctcgaccgag cgggtgtcga
     3841 agccctcagt cgatacgaac tgcttcttca gctggaactc cgtgaggtcg accccttcga
     3901 gctgcgggaa gtcgtcgggc agacccgagg gagagccgat cgaaggcggc aggctgcagg
     3961 acgcgagggg ttcaccctcg gcctcggtcg agcacgcgct cgagagcgac gcgacgaccg
     4021 ccgcggccac gagcacgccg catctgcgaa cgaaaaaccc agctttggac aattcgcgcg
     4081 gtccggaccg tattccgaag gcgtccaaag ctgggttttc gggtcaggcc ttcgcggcgc
     4141 ggcgcttgac gaacaggccg agggcgatga gggccgcgcc gagcgcgacg aacttcagga
     4201 tcgtggcacc ggtcgccgcg aggctcgatc gctcggcggg agtcgccgcc gcgtcgtctc
     4261 cgtcgtccgt tgcgtcgtcg cctccgcctc cgttcgactc gtcctcgtcc ccacctccac
     4321 cgcctccacc gggcgggtcg ccaccgtcgt tgccgccctg gttgccgtcg ttgccgcctc
     4381 tggacggctg cgcgatgaac gggatccctc cggactggtg gacgaagccc ggcagcgcgg
     4441 tcgccccgcc ttccgcagtg tcggagtaac caccccagac gtgcccggag ttgtcgatct
     4501 cgatcgtgaa gaagtctccg agtcgccggt cgacgagctc tgcctgacag gtcgtcccgc
     4561 cctgacagat cgtgctcgtg tggacggcgc gcggcgtcgc ggtcgcgacc ttgaagcccg
     4621 gcgacgacgc tcccttgcag ccgagcccgt tcagcgtctg cgcgacgtac acgcgccact
     4681 cctggtcgcc ctgggtctcg gcgtcgtggt tcgggagctc ctcgttcgcc ccgagccatg
     4741 ccacggcgac cttgccctcg tcaccgaccg cgagccacgg ccagatgtcg gtgttggcgt
     4801 tcttgtcgac gcgcgtaggc gccttccacg tccggccgcc atcctccgag tacgagaacc
     4861 agatgccggg gccgcgatcg ccctgtccgc tctcgtccca cgtcaggtag tggttgccgc
     4921 cggagtcgat gtcgaacgac ggccagtgcg acaacatgct gacgcccgag gcgacctcgt
     4981 gcgtgtcgcg gtcttcgagg ttccccttgc gggccttgcg gtaggcagtc atcgagagca
     5041 cgccgccgtc gtagtacgcc tcgtacagcg tgccgttcct gcgatcgatc tgaaggtcgc
     5101 cgagccccgc gccgtcgtcg acgccggcgg tgaacgtctg cccaccgtcg gtgctgcggt
     5161 gcaggaagtg accgctgttg
//
LOCUS       DASQDO010000467         6298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_906487, whole genome shotgun sequence.
ACCESSION   DASQDO010000467 DASQDO010000000
VERSION     DASQDO010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906487"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VEV43_12775"
     CDS             complement(<1..627)
                     /locus_tag="VEV43_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24440.1"
                     /translation="MAESKLQPVQEVDFPARATAIRDQLTQLTRERRAELREFSRSLV
                     TRIEKTLDYLLVISAAVDPHLVTERMVRAVTEELDDLNGGLENFADGGGVPYLQRIDI
                     ATDGVLDALGIWVGLINEPTYDAIRKAASAYRRSLGQQLINVRGDLRSLQQSFAAFQK
                     TQSEARREFENELASERERIEELISSVQQQTTQLSEAITAQASRAEQVS"
     gene            complement(809..1282)
                     /gene="smpB"
                     /locus_tag="VEV43_12780"
     CDS             complement(809..1282)
                     /gene="smpB"
                     /locus_tag="VEV43_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HYP24441.1"
                     /translation="MARGQDAGGTKLIAQNRKARHEYLIEDTVEAGLMLVGTEVKSCR
                     EGKVSLTDAYAAVRDGEAWLLQCHINPYSHGNRQNHDPMRPRKLLLHRSEIELLDVEV
                     SQGGRTLVPLRLYFKHGIAKAEIAVARGKKTYDKRQSEAKRDADRAMQRELGRRR"
     gene            complement(1288..2484)
                     /locus_tag="VEV43_12785"
     CDS             complement(1288..2484)
                     /locus_tag="VEV43_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYP24442.1"
                     /translation="MQQWVKVSLAVLGAVAVCMIAFSIGLAVGERGGGASGSAEGAAA
                     FGSGAGGDFDLVEEAYREIASSAVDSPGEEALARGAIKGMLEVLKKDDPYALFYSPNA
                     FRSFQELTSGQFSGIGVWLKEKDAGLTIVSVLPSTPALEAGLREGDVIETIDGDEATS
                     FTSDEAVALIKGPEGTDVSLGIERDGEELDFTITREEIDLPNLRASLRSDDVGYIQLF
                     GFARGAGAQVRDEVSELLDQGAEGIVLDLRNNGGGLFSEAVDVASVFIEDGEVVIYRE
                     ASDEETSYEAEGDAFEDVPLVVLVNEGTASASEIVAGALQDQERATLIGTTTYGKGSV
                     QEVVRLRDASALKLTTAAYLTPEGRDINGKGIDPDVEVTDVVEQKDRAVEILKGIIIS
                     TDGAQG"
     gene            complement(2503..3696)
                     /locus_tag="VEV43_12790"
     CDS             complement(2503..3696)
                     /locus_tag="VEV43_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012864280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYP24443.1"
                     /translation="MRKVTLGAALLLLAIALPAAADPGSELDRVRERQEEVEAQKEDK
                     LARAASVTDRLAELDRARERVEERVEDLASKVAALDAKIERTEAELTEAQKELAFLEA
                     RLDVIERRLDRKHDVLTDRVVEAYMEGPTGYLDSLLSSSDFGELVDRIEYRTSAMDTE
                     SQLIARIEVLQDKTEARERLAEEKKLEIAAAKATLEDDRREVDAKRDEHAAVLAEKEA
                     VLDEKRTLLADLRDDARRLAALEAQLEADESRLVSIIQAAVPTSAGPAPVGGGQLLIP
                     AAGPITSPYGYRTHPIFGDQRLHTGIDIGAGYGAPVVAADAGTVVYAGTMSGYGNVIA
                     IDHGGGLSTTYNHLSAFYVGSGQSVGRGTTIGGVGCTGYCTGPHLHFEVRINGSPVDP
                     MPYLQ"
     gene            complement(3804..4685)
                     /gene="ftsX"
                     /locus_tag="VEV43_12795"
     CDS             complement(3804..4685)
                     /gene="ftsX"
                     /locus_tag="VEV43_12795"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYP24444.1"
                     /translation="MRLRYFLGETVTNLRRNLLMTIAAISTVAISLLLLGGVQILGMI
                     VNKATLGWESKVEISAFLRADIADGELQAIQRDLAAMPEVEDIDYVTQDEAYEEFKAL
                     YPDQPHLYETIEPEDLPASVRVKLTDAKFTEEVSARIEGAPGIDEVRFGGDVIRRLLQ
                     INALLRTITLAMSLILLVASAGLIANTIRVAIYARREEIGIMKLVGATNWFIRIPFML
                     EGVFAALVGAAVSGAVVLAANAFLFPPLRDFLPFLRSVFAFSGGEIASVLLVLVAVGA
                     GVGIVGSSLALRRFLEV"
     gene            complement(4690..5376)
                     /gene="ftsE"
                     /locus_tag="VEV43_12800"
     CDS             complement(4690..5376)
                     /gene="ftsE"
                     /locus_tag="VEV43_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HYP24445.1"
                     /translation="MIRTVNVEKEYKGGIRALNGLSLDIEKGEFVFIVGPSGSGKSTF
                     LKLLTKEEEPTAGEVYVAGKNLASLPRWRVPYLRRNVGCVFQDFKLLPNKTVFENVAF
                     GLEVIGRPRAVVQRQVPQILELVGLGEKLDRFPDELSGGEQQRVSIARAFVNRPLILI
                     ADEPTGNLDPATSVGIMRLLDRINRTGTTIVMATHDHAIVDSMRRRVIELENGDVVRD
                     QSRGIYGVGT"
     gene            complement(5395..>6298)
                     /locus_tag="VEV43_12805"
     CDS             complement(5395..>6298)
                     /locus_tag="VEV43_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HYP24446.1"
                     /translation="AIDFSEDKVHWDAMTGAFEGFRTELQRSFTLFFVGEQAVTDTLA
                     PLVHAAPDEPSRIFLSTQLVDEARHSVFFSRFFEQVVGLPGGLSEALAHLRDRTVGGF
                     RAMFDEDLVQVTEAVRLAPHDYGAWIEAVTVYHLIIEGMLALTGQKFLLGFAREMGIL
                     PGFYTGFTAVARDESRHVNFGVRAIMEAGVRDRALLERVEDTVLRMLEDACRTVASPD
                     RKFAVSPALTPPNLLINPYEVASFSARSLTKRLRVAGVSAKVCGEIEQRAARYYQAAW
                     DEYEDLHGEEHPYRFFDRPTALAN"
BASE COUNT         1009 a         2160 c         2072 g         1057 t
ORIGIN      
        1 gctgacttgc tccgcgcgcg aggcttgggc cgtaatggct tccgaaagct gagtcgtctg
       61 ctgctggacg ctagaaatga gttcctcaat gcgctcccgt tcgctggcca attcgttttc
      121 gaattcgcga cgggcctccg attgtgtctt ttggaaggca gcaaacgatt gctgaagcga
      181 tctcaagtcg ccacgaacat tgatcaattg ctgcccaagg gacctccgat aggcggaggc
      241 tgccttcctg atcgcgtcgt acgtgggctc gttgataagg ccgacccata tcccaagcgc
      301 gtcaaggaca ccgtcggtcg caatgtcaat gcgttgcagg tatggaactc ctccaccgtc
      361 cgcgaagttt tcgaggcccc cgttgagatc gtccaactct tcggtgacgg cgcggaccat
      421 ccgctcggtc acgagatggg ggtcgaccgc agcagagatc acaagaaggt aatcaagcgt
      481 cttctcgatt cgcgtgacca acgaacgact gaattcacgg agttcggcgc ggcgctcgcg
      541 cgtgagctgc gtcagttggt cgcgaatcgc ggtcgctcgc gcggggaaat ccacttcttg
      601 aacaggttga agcttcgatt ccgccacatc gtcccctggt tctctgggca atcagtcctg
      661 aatagtaggg acgttcggtg ccgctgtcat cgccccggac agcaccaaat tccgcgagtg
      721 gactacatgt catcaggtgg acatcagcac gtactcggcg aagggccgcg acgggcagac
      781 gggtgacagc agctgaaacg atgccctgct agcgccggcg gcctagctcc ctttgcatcg
      841 cccggtccgc gtccctcttc gcctcggact ggcgtttgtc gtacgtcttc ttgccacggg
      901 ctaccgctat ctcggccttc gctatcccgt gcttgaagta cagccgtagc ggcaccagcg
      961 tgcggccccc ttgcgatacc tcaacgtcca gtagctcaat ctccgaacgg tgcaataaca
     1021 gcttgcgcgg gcgcatcgga tcgtggttct ggcggttgcc gtgcgagtac gggttgatat
     1081 gacattgcag cagccaggcc tcgccgtctc ggaccgctgc gtatgcgtcc gtcagcgaaa
     1141 ccttgccctc gcggcacgac ttgacctcgg tccccacgag catgagcccg gcctcgaccg
     1201 tgtcctcgat caggtactcg tgacgcgcct tgcggttctg ggctatcagc ttcgtcccgc
     1261 cggcgtcctg gccgcgcgcc acgaacccta gccctgggcg ccgtcggtcg agatgatgat
     1321 ccccttcagg atctcgacgg cccggtcctt ctgctcgacg acgtcggtca cctcgacgtc
     1381 ggggtcgatg cccttgccgt tgatgtcgcg gccctcgggg gtcaggtacg cggcggtcgt
     1441 cagcttcaga gccgacgcgt cgcgcaggcg cacgacctcc tgcaccgagc ccttgccgta
     1501 agtcgtcgtc ccgatgagcg tcgcgcgctc ctggtcctgc agcgcgccgg cgacgatctc
     1561 ggacgcgctc gcggtcccct cgttgacgag caccacgagc ggcacgtcct cgaacgcgtc
     1621 gccctcggct tcgtacgacg tctcttcgtc cgacgcctcg cggtagatca cgacctcgcc
     1681 gtcctcgatg aacacgctcg cgacgtcgac ggcctccgag aacagaccgc cgccgttgtt
     1741 gcggaggtcg agcacgatcc cctcggcccc ctggtcgagc agctccgaga cctcgtcgcg
     1801 cacctgcgcg cccgcacccc gtgcgaagcc gaacagctgg atgtagccga cgtcgtcgga
     1861 gcggagagac gcgcgcaggt tcgggaggtc gatctcctcg cgggtgatcg tgaagtcgag
     1921 ctcctcgccg tcgcgctcga tccccaggga tacgtccgtc ccctccggcc ccttgatcag
     1981 agcgacggcc tcgtcggacg tgaagctcgt ggcctcgtcg ccgtcgatcg tctcgatcac
     2041 gtcgccttcg cgcagcccgg cctccagcgc cggcgtcgac ggcagcaccg acacgatcgt
     2101 caggcccgcg tccttctcct tgagccacac gccgatcccg gagaactggc ccgacgtgag
     2161 ctcctggaac gagcggaacg cgttcggcga gtagaagagc gcgtaggggt cgtccttctt
     2221 caggacctcg agcatcccct tgatcgcgcc gcgggccagg gcctcctcac ccggagagtc
     2281 gaccgccgac gacgcgatct cgcggtacgc ctcctcgacc aggtcgaagt cgccgccggc
     2341 cccgctcccg aaagccgcgg ccccctcggc cgagccggac gcaccgccgc cacgctcgcc
     2401 cacggcgagg ccgatggaga acgcgatcat gcacacggcc accgcgccga ggacggcaag
     2461 cgacaccttc acccactgct gcatgcccta gagggtagtg ccctactgca ggtagggcat
     2521 cgggtccacc ggggacccgt tgatccgcac ctcgaagtgg aggtggggcc cggtgcagta
     2581 gccggtgcaa ccgacgccgc cgatcgtggt gccgcggccg acgctctgcc cgctgcccac
     2641 gtagaacgcc gacaagtggt tgtaggtcgt ggagaggccc ccgccgtggt cgatcgcgat
     2701 cacgttgccg tagccgctca tcgtcccggc gtagacgacc gtcccggcgt cggcagccac
     2761 gaccggcgcg ccgtacccgg cgccgatgtc gatcccggtg tggaggcgct ggtcgccgaa
     2821 gatcgggtgc gtccggtagc cgtagggaga ggtgatcggc cccgctgccg ggatgaggag
     2881 ctgtccgccg cccacggggg ccgggccggc ggaggtcggg accgcggcct ggatgatcga
     2941 cacgagccgg gactcgtccg cctcgagctg ggcctccagg gcggcgagcc ggcgggcgtc
     3001 gtcgcggagg tcggccagca gcgtgcgctt ctcgtcgagc acggcctcct tctcggcgag
     3061 cacggccgcg tgctcgtccc gcttggcgtc gacctcacga cggtcgtcct cgagcgtcgc
     3121 cttggcggcg gcgatctcga gcttcttctc ctccgcaagc ctttcgcgcg cctcggtctt
     3181 gtcctggagg acctcgatcc gggcgatcag ctgggactcg gtgtccatcg cggacgtgcg
     3241 gtactcgatc cggtcgacga gctcgccgaa gtccgacgac gagaggagcg agtcgaggta
     3301 gcccgtcggg ccctccatgt aggcctcgac aacccggtcg gtgagcacgt cgtgcttgcg
     3361 gtcgaggcgc cgctcgatga cgtcgagcct ggcctcgagg aaggcgagct ccttctgggc
     3421 ctcggtgagc tcggcctcgg tgcgttcgat cttggcgtcg agcgccgcga ccttcgaagc
     3481 caggtcctcg accctctcct cgacccgctc gcgggcccgg tcgagctcgg ccagccggtc
     3541 ggtcacgctc gccgcgcggg cgagcttgtc ctccttctgg gcctcgacct cctcctggcg
     3601 ctcccggacg cgatcgagct cgctaccggg gtccgccgcg gcgggaaggg cgatcgccag
     3661 aaggagcagg gcggcgccga gggtcacctt gcgcattggg ttcatcctaa gcaacaggtt
     3721 ccccattaac aacatcagaa acggacaaag gggatgctcc agccgagcct cccaggatgt
     3781 cgtcggccgt cgcggccggc gtcttaaacc tccaggaagc ggcgcagggc gagcgacgac
     3841 ccgacgatcc cgacgccggc ccccacggcc acgagcacga gcaggaccga cgcgatctcg
     3901 ccgcccgaga aggcgaacac gctccgcagg aacggcagga agtcccggag cggcgggaag
     3961 aggaaggcgt tcgcggcgag cacgaccgcg cccgagacag cggcccccac cagggcggcg
     4021 aagacccctt cgagcatgaa ggggatccgg atgaaccagt tcgtcgcgcc gacgagcttc
     4081 atgatcccga tctcctcacg gcgggcatag atcgcgactc ggatcgtgtt cgcgatcagg
     4141 ccggcagacg ccacgagcag gatcagcgac atggccagcg tgatcgtgcg cagcagcgcg
     4201 ttgatctgga gcaggcgccg gatgacgtcg ccgccgaacc tcacctcgtc gatgcccggg
     4261 gccccctcga tgcgcgccga gacctcctcg gtgaacttcg cgtcggtcag cttgacgcgc
     4321 acggacgcgg ggaggtcctc gggctcgatg gtctcgtaca ggtgcggctg gtccgggtag
     4381 agagccttga actcctcgta cgcctcgtcc tgcgtgacgt agtcgatgtc ctcgacctcg
     4441 ggcatcgccg cgaggtccct ctggatggcc tgcagctcgc cgtcggcgat gtctgcgcgc
     4501 aagaaggccg agatctcgac cttcgactcc cacccgagcg tcgccttgtt cacgatcatc
     4561 ccgaggatct ggacgccgcc gagcagcagc agcgagatcg cgaccgtcga gatcgcagcg
     4621 atcgtcatca agaggttccg ccgcaggttc gtgacggtct cgccgaggaa gtacctgagc
     4681 cgcacggtcc taggtcccca ctccgtagat gccgcgcgac tggtcgcgta cgacgtcgcc
     4741 gttctcgagc tcgatcacgc ggcgcctcat cgagtcgacg atcgcgtggt cgtgcgtcgc
     4801 catcacgatc gtcgtgcccg tccggttgat ccggtcgagc agccgcatga tcccgaccga
     4861 tgtagccggg tcgaggttcc ccgtcggctc gtccgcgatg aggatcagcg gacggttcac
     4921 gaaggcgcgc gctatcgaga cgcgttgctg ctccccgccg gagagctcgt cggggaaccg
     4981 gtcgagcttc tcgccgagcc cgacgagctc gagtatctgc ggcacctgtc gctggacgac
     5041 cgcgcgcgga cgcccgatca cctcgagccc gaacgcgacg ttctcgaaca cggtcttgtt
     5101 cgggagcagc ttgaagtcct ggaagacgca gccgacgttg cggcggaggt acgggacgcg
     5161 ccagcgcggc aggctcgcga ggttcttgcc cgcgacgtag acctcgcctg ccgtgggctc
     5221 ctcctctttc gtgaggagct tgaggaacgt cgacttgccg gagcccgagg gcccgacgat
     5281 gaagacgaac tcgcccttct cgatgtcgag cgagagcccg ttgagcgcgc ggatgccgcc
     5341 cttgtactcc ttctcgacat ttacggtcct gatcatggtt agagggtagc cacctcagtt
     5401 cgctagagca gtagggcggt cgaagaaccg gtaggggtgc tcctcgccgt ggagatcctc
     5461 gtactcgtcc caggcggctt ggtagtagcg cgccgcccgc tgctcgatct cgccacacac
     5521 cttagcggag acgcccgcga cccgcagtcg cttcgtgagc gaccgggccg agaacgacgc
     5581 cacctcgtac gggttgatca ggaggttcgg cggcgtcagc gcaggcgaga cggcgaactt
     5641 gcggtccggg gacgccacgg ttcggcacgc gtcctcgagc atgcgcagga ccgtgtcctc
     5701 gacccgctcg agcagggcgc ggtcgcgcac gccggcctcc atgatcgccc gcacgccgaa
     5761 gttcacgtgg cgcgactcgt cgcgggcgac cgcggtgaag cccgtgtaga acccgggcag
     5821 gatccccatc tcgcgcgcga agccgagcag gaacttctga cccgtaaggg cgagcatgcc
     5881 ctcgatgatc aagtggtaga cggtgacggc ctcgatccac gcgccgtagt cgtggggcgc
     5941 gaggcggacg gcttcggtca cctgcacgag gtcctcgtcg aacatggcgc ggaacccgcc
     6001 gacggttcgg tcccgcagat gtgcaagagc ctccgacaga cccccgggca atcccacgac
     6061 ctgttcgaag aagcgcgaga agaacacgga gtggcgcgcc tcgtcgacga gctgcgtcga
     6121 cagaaagatg cgcgacggct cgtcgggggc cgcgtgcacg agcggcgcga gggtgtcggt
     6181 cacggcctgc tccccgacga agaacaacgt gaacgaacgc tggagctcgg tgcggaagcc
     6241 ctcgaacgcg ccggtcatcg cgtcccagtg gaccttgtcc tcgctgaagt cgatagcg
//
LOCUS       DASQDO010000468         5930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1151233, whole genome shotgun sequence.
ACCESSION   DASQDO010000468 DASQDO010000000
VERSION     DASQDO010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151233"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VEV43_12810"
     CDS             <1..408
                     /locus_tag="VEV43_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24447.1"
                     /translation="DATGVELQELNPEIVTVPLKPGDAAGCVPLLEDGLVASATAPDV
                     LLASIADELGPGYEIVGEQLTTEPLAAALERGAAGWVDYVNKIITEFDQEGRWAAAYE
                     EHLAPLLGGEAPDPPNMTLEEAAALFPADLAES"
     gene            521..946
                     /locus_tag="VEV43_12815"
     CDS             521..946
                     /locus_tag="VEV43_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYP24448.1"
                     /translation="MEQLPQVSIRDLSRRPNEVLRRVTAGERLIVCRHKKPVATLQPL
                     DGYVFQPFDGTARDVFGWPVGGIYDEIAKLSEAQRVLLRDGYRRWRLWSGRVPSDVPG
                     DRRTMLSDLAVRGLAKKTEFGWELTGRGLAMHEALQGRD"
     gene            complement(943..1218)
                     /locus_tag="VEV43_12820"
     CDS             complement(943..1218)
                     /locus_tag="VEV43_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HYP24449.1"
                     /translation="MRRGPRLVRVTSAEVLHDFVVRLAFDDGTERKVDLERFLWGPVF
                     EPIRDDPDVFRQVRVDDEAGTIVWPNGADIDPVVLHGSAEPAPRLDP"
     gene            complement(1227..1487)
                     /locus_tag="VEV43_12825"
     CDS             complement(1227..1487)
                     /locus_tag="VEV43_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HYP24450.1"
                     /translation="MPRISSFYGISIYMYFHDHEPAHFHAACAGREARFAIDTLRPMD
                     PGFPRWARRRVQEWGLQHRPELRENWLRARRHAPLAKIDPLP"
     gene            complement(1523..2032)
                     /locus_tag="VEV43_12830"
     CDS             complement(1523..2032)
                     /locus_tag="VEV43_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYP24451.1"
                     /translation="MPKARALAGRRLTWALVGVLVVSALVTPSALSDASASGPRYRYN
                     KSEKCFMRKVNRVRSQNGHRRLNWDKQLGYVARRHAKTMANAGGVWHDQNLGRRVTRW
                     RMLGQNTGAGGRCRKLFRSFMDSAPHRANILGRWRFIGVGTEWKNNRLYVQTVFEWRR
                     NPGNVWSWP"
     gene            2276..2638
                     /locus_tag="VEV43_12835"
     CDS             2276..2638
                     /locus_tag="VEV43_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP24452.1"
                     /translation="MTAETTTATKTVLIVDDDEEIRHVLRLLCESEGLEVIGEAANGV
                     VAVPMALKHQPDFVILDFMLPRLDGEGAAEILRAVTPKSKIVAFSAILDSQPVWADAY
                     LNKDRITELMPLLRTFIR"
     gene            2649..3152
                     /locus_tag="VEV43_12840"
     CDS             2649..3152
                     /locus_tag="VEV43_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HYP24453.1"
                     /translation="MAKDSSFDVVSEVDLQELRNAVDQARREISQRFDFKNTGSEIDL
                     AEEKEAPVLTIRSNTDGKVKEVVRVLEEKLVKRQIALKALEKGEIEPAAGGTARQTIK
                     VNQGISTEKAKQIVKFIKDKKLKAQASVQGDQVRVSSKSRDILQDVIGSLKEEDFGIP
                     LQFTNYR"
     gene            complement(3136..3777)
                     /locus_tag="VEV43_12845"
     CDS             complement(3136..3777)
                     /locus_tag="VEV43_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYP24454.1"
                     /translation="MKYDALVVDFGGVLTTPLQEAMFRFAEEAGIELQHLARAALGGY
                     MGSEDSLVFGFETGHISQEEFTKAFAERLREESGVEVDPESLIDRLFGGLDLEESMFT
                     AVQRARAGGLKTGLLSNSWGTALYPRERLDPIFDVQVISGEVGLRKPDPAIFRLTTDR
                     LGVPAEKCVFVDDHPGHLTSAAEAGMTTVLHRDPARTIAELESLLEMSLSGSS"
     gene            complement(3774..4793)
                     /locus_tag="VEV43_12850"
     CDS             complement(3774..4793)
                     /locus_tag="VEV43_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYP24455.1"
                     /translation="MQLVDPEALEAYLAAAVPGEGDLVVQRHQAGHSNETFFVTRGES
                     RWVLRRPPQGAFLPSAHDVKREHTVLSGLAGTGVRVPRTVLMCEDESVIGVPFYLMER
                     VDGVVIRDELPDWLDERQRHLVGSELVDALVELHSVDPGSCGLGGFGKPSGYLERQLR
                     RWKGQMELTLPFTRPLPDLEAIGEWLAAHRPPDAETTLVHGDYKLDNVVFADGPPARL
                     VAILDWEMSTLGDPLADLGWMVSFWREAGEDEEDVFTRTARVTALPGFASRADLVERY
                     ATKTGRDVSNLNWYQVLAVWKLAILLEGSYARHLAGMTDDPFFAEMEHGVPALAARAL
                     EATRS"
     gene            4817..5542
                     /gene="coxB"
                     /locus_tag="VEV43_12855"
     CDS             4817..5542
                     /gene="coxB"
                     /locus_tag="VEV43_12855"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYP24456.1"
                     /translation="MTRVSVRTAVAVAAVAATAGCAPEAAGEQGAAVKDLYDVFFYVA
                     AVVFAVVAGLIAWSIVRFRKRPGDDEAPPQTKHNLALEITWFAIPTAIVVVLFVLSAG
                     ALQDVNEEHDDPTVTVQVDAFQWGWRFTYADEAVVVEGLPEEPARIVLPVDETLRFDL
                     RSRDVVHSFYVPRFLVKRDTVPGETNRIDYLIREEGTYDGRCAEFCGVLHDSMPFTIE
                     AVPMDEFEGWLAEQRTENEDAGI"
     gene            5529..>5930
                     /locus_tag="VEV43_12860"
     CDS             5529..>5930
                     /locus_tag="VEV43_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYP24457.1"
                     /translation="MPASEVAAVAVHAEPRGLVAWLTTTDHKKIGVMYMASAFVFFVL
                     AGALSLVMRAELAAPELQFLDLGTYNQLFTMHGTAMIFFFATPMVVGLANYFVPLQIG
                     APDVAFPRMNAFTYWLFIFSAVIVFLGFFVTA"
BASE COUNT          989 a         1971 c         2025 g          945 t
ORIGIN      
        1 gacgcgaccg gcgtggagct ccaggagctc aaccccgaga tcgtcacggt cccgctgaag
       61 cccggcgacg cggcgggctg cgtccccctc ctcgaggacg gcttggtggc gtcggccacg
      121 gcccccgacg tcctgctggc ctccatcgcc gacgagctcg gccccggcta cgagatcgtc
      181 ggcgagcaac tgacgacgga gcccctggcg gccgccctcg aacgcggcgc ggccggctgg
      241 gtcgactacg tgaacaagat catcacggag ttcgaccaag agggccgctg ggccgccgcc
      301 tacgaggagc acctggcccc cctcctgggc ggcgaggccc cggacccgcc gaacatgacg
      361 ctggaagaag cagcggccct cttcccggcc gacctagccg agagctaact acgcccacac
      421 ctcgcgcgac cccctcggaa tttgtcagac atttgtctga caaatttcca gggcccggta
      481 cggacacgtt cttgtcatag gtcaccgcta gcttcggccc atggagcagc taccgcaggt
      541 gtcgattcgg gatctcagcc ggcgtccgaa cgaggtgttg cgacgtgtga ccgcgggtga
      601 gcggttgatc gtgtgcaggc acaagaagcc ggtggcgacg ctgcagccgc tcgacgggta
      661 cgtgttccag ccgttcgacg ggacggcacg cgacgtcttc gggtggccgg tcggtgggat
      721 ctacgacgag atcgccaagt tgagcgaagc gcagcgcgtc ctactgcggg acggctacag
      781 gaggtggcgg ctgtggtccg ggcgcgtgcc ctccgacgtc ccgggggatc ggcgcacgat
      841 gttgagcgac ctcgcggtcc gggggctggc gaagaagacc gaattcgggt gggagctgac
      901 gggccggggc ctcgctatgc acgaagcctt gcaggggcgc gactaggggt cgagtcgcgg
      961 cgcgggttcg gccgatccgt gcaacacgac gggatcgatg tcggcgccgt tcggccacac
     1021 gatcgtgccc gcctcgtcgt ccacccgcac ctgacggaag acgtcggggt cgtctctgat
     1081 cggttcgaac accggccccc agaggaaccg ctcgaggtcg accttcctct cggtgccgtc
     1141 gtcgaacgcg agccggacga cgaagtcgtg gaggacctcg gcggaagtga ctcggaccag
     1201 gcggggtccc cgcctcaata gacgtctcat ggcagtggat cgatcttagc cagcggcgcg
     1261 tgccgtcgcg ctctcagcca gttctcccga agctccggac ggtgctgcaa gccccattcc
     1321 tggacacgcc gccgagccca gcgtgggaac cccggatcca tcggacggag tgtgtcgatc
     1381 gcgaatcgcg cctcgcgtcc cgcgcaagcc gcgtggaagt gcgccggctc gtggtcgtgg
     1441 aagtacatgt agatcgagat cccgtagaag ctgctgatcc tcggcacctc gggaaggtac
     1501 cgacggggta cgacggaaag agctagggcc aggaccagac gttgccgggg ttccggcgcc
     1561 actcgaagac cgtctgcacg tataggcggt tgttcttcca ttccgtcccc acgcctatga
     1621 agcgccagcg gcccaggatg ttcgcccggt gcggggccga gtccatgaac gaacggaaca
     1681 gcttgcggca gcggcctccg gcccccgtgt tctggccgag catgcgccaa cgcgtcaccc
     1741 ggcggccgag gttttggtcg tgccacacgc cgccggcgtt cgccatcgtc ttcgcgtggc
     1801 ggcgggcgac gtagccgagc tgcttgtccc agttgaggcg gcggtggccg ttctgcgagc
     1861 ggaccctgtt caccttgcgc atgaagcact tctcggactt gttgtagcga tagcgggggc
     1921 cgcttgcgga cgcgtcgctc agggcggacg gcgtcacgag ggcggaaacg acaaggacac
     1981 cgacgagcgc ccaggtcagg cgcctgccgg cgagcgcgcg cgccttcggc atgtgcaacc
     2041 tcctgtatgg gggattttga gggacttgcc cgcgatgtcc caaaaactcc ctctatttag
     2101 caacggagcg gcgtcgtgtc cagtcaaccc gtatacgcag agtgatgccg cgcatccatt
     2161 gtgtaaggag gtgcaggtca gacgccggaa ataaacctag aaaaaagttc gcgtgcccta
     2221 cgggggccgc gaggggtgga caaacggtcg cgctttttgc aagcctgtga ccccaatgac
     2281 cgccgagacg accaccgcga ccaagaccgt cctcatcgtc gacgacgacg aggagatccg
     2341 tcacgtcctg cgcctcctct gcgagagcga gggcctcgag gtcatcggcg aggccgccaa
     2401 cggcgtagtg gccgtcccga tggcgctcaa gcaccagccg gacttcgtga tcctcgactt
     2461 catgctgccg cgactcgacg gcgagggcgc ggccgagatc ctgcgcgcgg tcacgccgaa
     2521 gtcgaagatc gtcgccttct ccgcgatcct cgacagccag cccgtctggg ccgacgccta
     2581 tctcaacaag gaccgcatca cggagctcat gccgctgctc cgaacgttca tccgttagat
     2641 tccccggcat ggccaaagac tcgtcgttcg acgtcgtgtc cgaggtcgac ctccaggagc
     2701 ttcgcaacgc ggtcgaccag gcgcgcagag agatctcgca acgcttcgac ttcaagaaca
     2761 cggggtccga gatcgacctc gcggaggaga aggaagcccc ggtcctgacg atccggtcga
     2821 acaccgacgg gaaggtcaaa gaggtcgtgc gggtcctcga ggagaagctc gtcaagcggc
     2881 agatcgcgct gaaggcgctc gagaagggcg agatcgagcc cgccgccggc ggcaccgccc
     2941 gtcagacgat caaggtcaac cagggcatct cgaccgagaa ggcgaagcag atcgtcaagt
     3001 tcatcaagga caagaagctg aaggcgcagg catccgtcca gggcgaccag gtgcgcgtct
     3061 cgtcgaagtc acgcgacatc ctgcaggacg tgatcgggtc gctgaaggaa gaggacttcg
     3121 ggatcccgct ccagttcacg aactaccgct gaggctcatc tccagcaacg actccagctc
     3181 cgcgatcgtc cgcgccgggt cacggtggag cacggtcgtc atccccgctt ccgccgcgct
     3241 cgtcaggtgg ccggggtggt cgtctacgaa gacgcacttc tccgccggca cgccgaggcg
     3301 gtccgtcgtc agccggaaga tcgccgggtc gggtttgcgc aggccaacct cgccggagat
     3361 gacctgcacg tcgaagatcg ggtcgaggcg ctcgcgggga tagagcgccg tcccccacga
     3421 gttcgatagg aggcctgtct tgaggccccc cgcccgggcc ctctggaccg cggtgaacat
     3481 gctctcctcc aggtcgaggc cgccgaacag ccggtcgatc agcgactccg ggtcgacctc
     3541 gacgccggac tcctcgcgca gccgctccgc gaacgccttc gtgaactcct cctgggagat
     3601 gtggccggtc tcgaagccga acacgagcga atcctcggag cccatgtacc cgccgagcgc
     3661 ggcacgggcg aggtgctgga gctcgatgcc ggcctcctcg gcgaagcgga acatggcctc
     3721 ttgaagcggc gtggtgagaa cgccgccgaa gtcgacgacc agggcgtcgt atttcacgag
     3781 cgtgtcgctt ccagggctcg cgccgcgagg gccgggacgc cgtgctccat ctcggcgaag
     3841 aacgggtcgt cggtcatgcc ggcaaggtgg cgcgcatagg acccttcgag gaggatcgcc
     3901 agcttccata ccgcgaggac ctggtaccag ttgaggttgc tcacgtcgcg tccggtcttc
     3961 gtggcgtatc gctcgacgag gtcggcgcgc gaagcgaaac cgggcagcgc cgtgacgcgg
     4021 gccgttctcg tgaagacgtc ttcttcgtcc tcgcctgctt ctctccagaa cgagaccatc
     4081 cagccgaggt cggcaagcgg atcgccgagc gtcgacatct cccagtcgag gatcgcgacg
     4141 agacgcgccg ggggcccgtc cgcgaacacg acgttgtcga gcttgtagtc gccgtggacg
     4201 agcgtcgtct cggcgtccgg cgggcggtgc gcggcgagcc actcgcctat cgcctcgagg
     4261 tccgggagcg gtcgcgtgaa cgggagcgtc agctccatct ggcccttcca gcggcggagc
     4321 tggcgctcga ggtagcccga cggcttgccg aaaccgccga gcccgcagga cccggggtcg
     4381 acggagtgga gctcgacgag cgcgtcgacg agctcggagc cgaccagatg gcgctgccgc
     4441 tcgtccagcc agtccgggag ctcgtcgcgg atcacgacgc cgtcgacgcg ctccatcaga
     4501 tagaacggca cgccgatcac gctctcgtcc tcgcacatca ggacggtccg ggggacgcgg
     4561 acgcccgtgc cggcgagccc cgacaggacc gtgtgctctc tcttgacgtc gtgcgccgac
     4621 gggaggaacg cgccctgcgg tgggcggcgc agcacccagc gcgactcgcc gcgggtgacg
     4681 aagaacgtct cgttcgagtg gcccgcctgg tgacgttgca ccacgaggtc gccctcgccg
     4741 ggaacggctg cggcgagata tgcctcgagc gcctcgggat cgaccagctg catggggtca
     4801 aagtaccctc ggcccgatga cccgcgtgtc cgtgcgcacc gccgtcgccg tggctgcggt
     4861 cgcggccacg gccgggtgcg ctcccgaagc ggcgggcgag cagggggccg cggtcaagga
     4921 cctctacgac gtcttcttct acgtcgcggc ggtcgtcttc gccgtagtcg cggggctgat
     4981 cgcctggagc atcgttcgct tccgcaagag acccggcgac gacgaggcgc ctccgcagac
     5041 gaagcacaac ctcgcgctgg agatcacgtg gttcgcgatc cctaccgcga tcgtcgtggt
     5101 gctgttcgtg ctctcggccg gcgcgctgca ggacgtgaac gaggagcacg acgacccgac
     5161 cgtgaccgtg caggtcgacg cgttccagtg ggggtggagg ttcacctatg ccgacgaggc
     5221 cgtggtcgtc gaggggctcc ccgaggagcc cgcgcggatc gtgctgccgg tcgacgagac
     5281 gctgcgcttc gacctgcgct cgcgcgacgt cgtgcactcg ttctacgtcc cgcgcttcct
     5341 ggtcaagcgc gacacggttc ccggcgagac gaaccggatc gactacctga tccgcgagga
     5401 gggaacctac gacgggcgct gcgccgagtt ctgcggcgtg ctccacgact cgatgccgtt
     5461 cacgatcgag gcggtcccga tggacgagtt cgagggatgg ctcgcggagc agaggacgga
     5521 gaacgaagat gccggcatct gaggtggccg cggtcgcggt ccacgccgag ccgcggggcc
     5581 tggtcgcgtg gctgacgacg accgaccaca agaagatcgg cgtcatgtac atggcgtcgg
     5641 ccttcgtgtt cttcgtcctc gcgggcgcgc tgtcactggt gatgcgcgcc gagctcgcgg
     5701 cacccgagct ccaattcctt gacctcggca cgtacaacca gctgttcacg atgcacggca
     5761 ccgcgatgat cttcttcttc gcgacgccca tggtggtcgg gctggcgaac tacttcgtgc
     5821 cgctgcagat cggcgccccg gacgtcgcct tcccgcggat gaacgcgttc acctactggc
     5881 tgttcatctt cagcgcggtg atcgtcttct tagggttctt cgtgaccgcg
//
LOCUS       DASQDO010000469        12937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1028893, whole genome shotgun sequence.
ACCESSION   DASQDO010000469 DASQDO010000000
VERSION     DASQDO010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028893"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2580)
                     /locus_tag="VEV43_12865"
     CDS             complement(<1..2580)
                     /locus_tag="VEV43_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYP24458.1"
                     /translation="MAVELSAAQRGLWALDQLAPSSAFYNVAAAYRLHGPLDVDALGA
                     ALATVVERHEVLRTSIESAGGVPAGVVRDAGPALAVDDLSGVAPSRRDAEALRAAAAE
                     AARPFDLAAEAPFRARLLRLAPADHVLSVTAHHVAADEASLRTVLGEVSRAYAGEALP
                     EAPLQYAEIAAREAGVLHGEDLDVLLAFWRKTLRDAPARMRLPFDHPAPAVPAFRGAA
                     VETHVEPSVVAALESVAPLETVLLAAFGVLLHRYTSADDLVVGWPVDARTGSTRDVVG
                     TFVNLLPLRLRVRGDDSFRDVVGRVDETVQAARAHRELPLETLIEELRPRRGPGHMPL
                     FQVAVSVGDEPQEALSLVDVAVTPIPLEPATAQLDLFLDVKRSSEGLRARFELSTDVF
                     DPPTIHALSRHWENLLRAIAGDPDGRVVSLALLDDEEAAGLLRDADGGRAPEPAGLVP
                     DMVVERARLRPDAVAVVAPDGELTYGELDARSAAIAAGLAERGIGRGSVVALLVERSV
                     AMVPAMLGILRAGCAYLPLDPSYPKERLRFLVEDSGAAAVIGQEGPPEAQPRRPYEIA
                     PRDAAYVIYTSGSTGTPKGVVVEHAALARFVATISEELALTESDRVLQFASLSFDTAV
                     EEIWPALVAGATLVLRDPHPWHPEEMRDKVAEHGITVLDLPTAYWHEVAGACADGIDV
                     RDAPSLRLVVVGGEAMSADATRLWSSSGTGARLLNTYGPTETTVTATSFDATSWHDSV
                     AVPIGRPLPGVRAYVLDADSQPVPPGVTGELFVGGAGVARGYLGRPGATAERFVPDPW
                     SPDPGARMYATGDLVRRGRDGVLYFAGRRDDQVKIRGFRVEPGEVEAALAAHPAVADA
                     AVVV"
     gene            complement(2570..12658)
                     /locus_tag="VEV43_12870"
     CDS             complement(2570..12658)
                     /locus_tag="VEV43_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYP24459.1"
                     /translation="MTYEDVAIVGISGIFPEAGDLAELYRNLAGGRDSVRALPRERVE
                     TTSVDPNLDYPTVGCLDRVDLFDHKFFNISLREAEYMDPHQRVTLELVCAAVENAGYS
                     LASLRGTRTGVFLSAPRPEYYELFRDVDPLELLGNAPSALAGRISYVLDLHGPSLVMD
                     AGCCGSLVAVDAACRDLLAGEARFAIAGGVALTVLFEPSTTTAAFTEIMSPDGKCRAF
                     DASADGTAAGEGGAVVVLKLLRHALEDGDQVHAVIKGVAVNQNGYRSNGLSAPSPAAQ
                     AEVIRAAWANARVEPASIGYVEAHGSGTRLGDVIEVQGLVEAFRGSGRTAPCPVGSVK
                     TNVGHLDHAAGIAGMVKAILSLKHAQLFPSLHYESPSPLIDFEDAVRVQDEPAPWAAA
                     SPRRAGVSSFSLAGTNVHMVLEEAPARPAPSTRDDAELVTISAKSGAALRDYCRSFAS
                     FVRDTEHDLATVAATLNRSRDDHQVRAAAVVASKDELVSWLEATAFGAESLQPVPREA
                     RPLALLFSGDEEIPGQAFETMLDHLSPVSSAVEMPEGAAPGLRLFGWHQALHSLIRSL
                     GLTEASVLGSGAGNLTVRTLVAGLDVEDAAREAAVVQPSAQVDVEKLAAAVTRLGSDV
                     VFLEMGGDGVLGRSVRAVRPEADVMPLLRGGRSALHALAELYARGVAIDWDRYYEGRE
                     LPRIEAPTYPFQRERCWCRPVGDYRRGGDDHAPGEVRALLESPEVAPSGAFSTDTERV
                     LALIWNEALKVEPDDASADYFDLGGTSITGMVVLDGVARDFGVQIAFQDLYEHSRLDQ
                     LAARIDALAPTAGAADGEPPIVPIPRGGYLPVSYGQEQIWFLDQLEPGTPLYNIPFDL
                     HLEGELDRPALEKALEDLARRHEVLRMGFVAVEGEPRAFVEPEPRVELPFVDLSSLPR
                     DEARAETLRLFDEEATTPFDLSRPPLFRGTLVALGEGDHVLLMTFHHIVYDGWTPSII
                     QDELSALYDAAKEGRDARLPVLPIQYPDFAQWQREWMSGETLQKGLDFWTAHLAGAPP
                     LDLPTDRPRPAVQSYAGGMVSLWIDERVADGLRALSRREGVTMFTTMMAAFDVLMARW
                     SGQDDVVVGTPTSGRKKAETRGLIGYFNNMLPLRVDLGGDPSFTDLMQRVKTVAAQAL
                     DHDDVPFETRVSALGEGRDLARNPLFQVAYSHQNAPQEGYSLKGLAVSTFGEGDIRGI
                     APGTAKFDLTLGIGDAGEGELEGYLEYATDLFDEDTVEAMIAHLQVLLEAVVADPSRP
                     LSRLPLVGPDESRALVEEPNPPLPERSTGPYVHERVAARAEAHPDEVALLFGDASLTW
                     GELHERAGVVAGALRSRGVGRGDVVAVAYPRSFEQVVSALGVLYAGAAYLPLDPAYPS
                     ARLQLMIRDASPAAVLAAEEVRTALGDVAYVDVSSSGEPAQPAAVDPADLAYVIYTSG
                     STGTPKGVAVTHSNLLQLVDWHAAAFPLEPGMRASTVAPLTFDASVWELWSALANGAT
                     LVLVDDDTRRDPDALVEEWRRHEVAAAFVSTVFAAPVLARPVPASLRTLFVGGDKLTV
                     RPPHDYGARVVDLYGPAEATVICTAGEVTSDGDGVPHIGRATTGARVYVLDADLNPLP
                     RGLPGEIYVGGRGVARGYLGRPGPTAERFLPDPFSAEPGARMYRTGDRARIRRDGNVD
                     FLGRVDEQMKVRGHRREPAEVEAALLALPSVEDGAVAVRAGRLVAYVVGDAETPEVRS
                     SLGEVLPAHMVPAAVVTLDALPVTPGGKLDRSALPEPGPERPEATYDAPETETEAALA
                     EVWAEVLRVDRVGRKDNFFDLGGDSILSIQIVARAAARGIAIAPKHVFEAQTVADLAR
                     VVETSKPSPGAPSERVTGAVPLTPVQRWFFEQDLAEPHHFNQAMLFELRAPADARALE
                     GALRALAEHHDVLRTRWLRDGSGWRGVIAPEEDGELLWTVDLSGRDPASAMLAMEAEA
                     ARLQASFDLAQGPLLRGALFDFGPLQPPQLLLTIHHLVVDAISWGVLVHDLSIAYGEL
                     VRGRAVELPAKTTSFQEWARHLESRVAEIDAAEKAMWRDRVSGLEPALLRRDRDGVND
                     VGSTSEVSFELDEATSNALVDAIARDRDVLPNEALIAILGRALGAISDGRHAVVDVEG
                     HGREAGTSGLELSRTVGWFTTLFPVVVEPGSSALAALESVRGQMRSAPGGGIGYGLLR
                     YLGTPADAGPLRDAPQPEVSFNYLGRYEGSIGGSGDLEPAEGPVGPLRSPAGARRHLI
                     EAEAWVAEGRVRVDLVYSSEVHDRSTIEALRDAFVAAAEEAVAAATGQATEADVDAVA
                     SRPGVADAYPLSPMQQGLLFHSLMGTAGSLYFEQLVLTITGPLDPAGLDRAWQQVAAR
                     HPLLAGTVEWEGIPEPMMLVPPEPRIEVTQNEVTDLDRFLAEDRARGFDLTEALTRVS
                     LIRVDDATWRMVWSHHHLLLDGWSVQLVLGELMDLYDDPAARLEPPGDYKSFVEWLRA
                     ADPAAAAAYWREALAGLEAPTDLRLVPPATPGEGHGMVVRTVNEAETESAAAFARAAR
                     VTLNTVVEAAWAATLGVYSGSDDVLFGAIMSGRSAPVERVERTAGLFINTVPIRTHIP
                     ASETVGEWLKKLQAAALERRDHEHAPLVEVQAQSAITAPTPLFDTLIAFENYPLDDVW
                     DEDEGDVRIEGAPASENTNYPISMVIAPGDGLTFRLSFDRARVDERAATALLDRFATM
                     LRAIVESPERRVGDLTALTEADRVTLAGFNETAAEYGNGLLVHEMFEAQAAARPDAEA
                     VACGPDSITYAALNERAGALAGHLADLGVGPDSLVAICCERTIEMVAGMLAVLKAGAA
                     YVPVDPRYPAERITYMLEDTAAPVLLTDRRVAERIPAGAAKVVLLDAANGNGHRSVEH
                     GRAAPDNLAYVLYTSGSTGRPKGIGIEHRSVKAFLDWAIATWRDDLRVVMACTSISFD
                     VHVFEIFGTLCSGGTVLLVEDALAFTTLERRGEMTLLNAVPSAVDELVSAGPLPPGIK
                     TINLPGEPLNRKLVERIFAASSAERIVNLYGPTEDTTYSTAAVIEPGEQGPISIGRPI
                     ANSWIYLLDRNLTQVPPGVPGEVYIGGAGLARGYFGRPGMTAERFLPDVVSTRPGGRM
                     YKTGDLARYRPDGSLDFLGRLDYQVKVRGFRVELGEIEQQLLEHPSVGHCVVTVRDAA
                     SGNRRIVAYVTGEGVDPGALRAHLAARVPDYMVPSAFVVLDALPLNPNGKIDRAALPD
                     PEAVRVPASGDDGALRTATEKVLASIWRELLEVGDVSRGDSFFDLGGQSLLATRLVSR
                     IRETWGCDARLRVVFDAPTLADLAREVETLCGGADAAEEVSAAVAEIESLSEEDVKRM
                     LAAMEGEVPDGG"
     gene            12736..>12937
                     /locus_tag="VEV43_12875"
     CDS             12736..>12937
                     /locus_tag="VEV43_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24460.1"
                     /translation="MLEELGYRERARVELVCAAVENAGYSLASLRGTRTGVFLSAPRP
                     EYYELFRDVDPLELLGYAPSALA"
BASE COUNT         1844 a         4739 c         4620 g         1734 t
ORIGIN      
        1 gacgaccacg gccgcgtcgg ccaccgccgg gtgcgcggcc agcgcggcct cgacctcgcc
       61 tggctcgacg cggaaaccgc ggatcttcac ctggtcgtcc cggcggccgg cgaagtagag
      121 cacgccgtcg cgcccgcgcc ggactaggtc tccggtcgcg tacatgcgcg cgcccgggtc
      181 gggcgaccac gggtcgggga cgaacctctc ggcggtcgcg cccgggcggc cgaggtagcc
      241 ccgggctaca ccggctcccc cgacgaagag ctcgccggtc acgccgggcg gcaccggttg
      301 ggaatccgcg tcgaggacgt aagcgcgcac gccgggcagg ggacgcccga tcggcacggc
      361 gaccgaatcg tgccacgacg tcgcgtcgaa ggacgtcgcc gtgacggtcg tctcggtagg
      421 gccgtaggtg ttgagcagcc gcgcccccgt cccggacgag ctccacagcc tggtcgcgtc
      481 cgcggacatc gcctcgccgc cgaccaccac cagccgcagg gacggcgcgt cccgcacgtc
      541 gatcccatcc gcgcacgctc cggcgacctc gtgccagtac gccgtcggga ggtcgagaac
      601 cgtgatcccg tgctcggcga ccttgtcgcg catctcctcg gggtgccacg gatgcggatc
      661 gcgcaggacg agcgtggccc cggcgacgag agccggccag atctcctcga ccgcggtgtc
      721 gaagctcagc gacgcgaact gcagcacccg gtcggactcc gtcagcgcga gctcctcgga
      781 gatcgtcgcg acgaatcgag cgagggccgc gtgctcgacc accacgccct tcggcgtccc
      841 cgtcgagccc gacgtgtaga tgacgtaggc ggcgtcacga ggagcaatct cgtagggccg
      901 ccggggctgg gcctccggcg ggccctcctg tccgatcacg gcggccgccc ccgagtcctc
      961 gaccaggaac cgcagccgct cctttgggta cgacgggtcg agcggcaggt atgcacagcc
     1021 ggccctgagg atcccgagca tcgccgggac catcgcgacg gagcgctcga cgaggagggc
     1081 gacgaccgag ccgcggccga tcccgcgctc ggcgagcccg gccgcgatag cggcggaccg
     1141 ggcgtcgagc tctccatagg tcagctctcc gtccggcgcg acgaccgcta cggcgtcggg
     1201 acgcaagcgt gctctctcga cgaccatgtc cgggacgagc ccggcgggct ccggcgcgcg
     1261 gcccccgtcc gcgtcccgca gcagccccgc cgcctcctcg tcgtccagga gcgcgagcga
     1321 cacgacgcgt ccgtccgggt cgcccgcgat cgcgcgcagc aggttctccc agtgacggct
     1381 cagcgcatgg atggtcggcg ggtcgaagac gtcggtcgag agctcgaagc gggcgcgcag
     1441 cccctcggac gaccgcttca cgtctaggaa gaggtcgagc tgtgcggtcg cgggctccag
     1501 cggtatcggc gtcaccgcga cgtccacgag cgagagggcc tcctgcggtt cgtcgccaac
     1561 cgacacggcg acctggaaca acggcatgtg gcccggcccc cgccgggggc gaagctcctc
     1621 gatcaacgtc tcgagcggga gctcccggtg cgcccgagcc gcctgcaccg tctcgtccac
     1681 ccgcccgacg acgtctcgga aggaatcgtc gccgcgcacg cgcaggcgca gcggcaagag
     1741 gttcacgaac gtccccacga cgtctcgggt cgagcccgtg cgagcgtcga ccggccaccc
     1801 cacgacgagg tcgtcagcgg acgtgtaccg gtgcaacagc acgccgaacg cggcgagcag
     1861 cacggtttcg agcggagcga cgctctccag tgcggccacg accgagggct ccacatgcgt
     1921 ctccaccgcg gcccccctga acgcggggac ggcgggcgcg gggtggtcga acgggagccg
     1981 catgcgagcc ggcgcgtcgc gcagggtctt gcgccagaac gcgagcagga cgtcgaggtc
     2041 ctcgccgtgc agcacgccgg cctcccgcgc cgcaatctcg gcgtactgga gcggagcctc
     2101 cggcagcgcc tcgcccgcgt aggccctgga cacctctccg aggaccgtgc gaagcgaggc
     2161 ctcgtccgcg gcgacgtgat gagcggtcac cgaaagcacg tggtcggcgg gcgcgagacg
     2221 cagcagccgc gcgcggaacg gtgcctccgc ggccaggtcg aacggccgcg cggcctcggc
     2281 ggccgcagcg cgcagagcct cggcgtcgcg ccggctcggc gcgacccccg agaggtcgtc
     2341 gaccgccagt gcaggcccgg catcgcggac caccccggcc ggtacgcccc cggcggactc
     2401 gatgctggtg cgcaggacct cgtgccgctc gaccacggtc gccagtgcgg cccccagcgc
     2461 gtcgacgtcc aggggcccgt gcagccggta ggcggcggcg acgttgtaga acgcgctcga
     2521 cggcgcgagc tgatcgaggg cccagagccc gcgctgcgcc gcggacagct caaccgccat
     2581 ccggcacctc gccctccatc gctgcgagca tccgcttgac gtcttcctcc gacaggctct
     2641 cgatctcggc tacggccgcc gagacctcct cggctgcatc cgcccctccg cacagcgtct
     2701 cgacctcgcg cgcgaggtcg gcgagcgtcg gcgcgtcgaa gaccacgcgc aggcgcgcgt
     2761 cgcagcccca cgtctcgcgt atccgcgaca cgagccgcgt cgcgagcagc gactgtccgc
     2821 cgaggtcgaa gaagctgtcc ccgcgtgaca cgtcgccgac ctcgaggagc tcgcgccaga
     2881 tcgaggcgag gaccttctcg gtcgcggtcc tgagcgcgcc gtcgtcgccc gacgccggca
     2941 cgcgtaccgc ctcgggatcg ggcagcgcgg cccggtcgat cttcccgttc gggttcaggg
     3001 gcagcgcgtc gagcacgacg aaggccgacg gcaccatgta atcgggcacg cgtgcggcga
     3061 gatgcgcacg aagcgcaccc gggtcgacgc cctcgccggt cacgtaggcg acgatccgcc
     3121 tgttgcccga cgcggcgtcg cggaccgtga ccacgcagtg cccgaccgaa ggatgctcga
     3181 ggagctgctg ctcgatctcg ccgagctcga cgcggaaccc gcggaccttc acctggtagt
     3241 cgagccggcc caagaagtcg aggctcccgt cggggcggta gcgcgcgagg tcgccggtct
     3301 tgtacatccg gcccccgggc cgcgtggata cgacgtcggg aaggaaccgt tccgccgtca
     3361 tccccgggcg cccgaagtag ccgcgggcga gccccgcgcc gccgatgtag acctcgcccg
     3421 gaacgcccgg cggcacctgg gtcaggttgc ggtcgagcag gtagatccac gagttcgcga
     3481 tggggcgtcc gatcgagatc ggcccttgct cgccgggctc gatcaccgcg gcggtcgagt
     3541 acgtcgtgtc ctcggtcggg ccgtagaggt tgacgatcct ctcggcgctg ctcgccgcga
     3601 agatccgctc gacgagcttg cggttcaggg gctcgcccgg caggttgatc gtcttgatgc
     3661 ccggcggcag ggggcctgcg ctcacgagct cgtcgacggc ggaaggcacc gcgttcaaca
     3721 gcgtcatctc gccgcgccgt tcgagcgtcg tgaacgcgag cgcgtcctcg acgagcagga
     3781 cggtccctcc ggagcacagc gtcccgaaga tctcgaacac gtgcacgtcg aacgagatcg
     3841 aggtgcacgc catgacgacg cgcaggtcgt cgcgccacgt cgcgatggcc cagtcgagga
     3901 acgccttcac gctgcggtgc tcgatcccga tgcccttcgg ccgtccggtc gagcccgacg
     3961 tgtacaggac gtacgcgagg ttgtccgggg cggcgcgccc gtgctcgacc gagcgatgcc
     4021 cgttcccgtt ggcggcgtcg agcagcacta ccttggcggc cccggccggg atgcgctcgg
     4081 cgactcggcg gtcggtgagc aacacggggg ccgcggtgtc ctcgagcatg tacgtgatgc
     4141 gctccgccgg atagcgggga tcgaccggca cgtacgcggc gccggccttc aacaccgcga
     4201 gcatcccggc gaccatctcg atcgtccgct cacagcagat cgcgacgagg gagtctgggc
     4261 ctacgccgag gtccgcgagg tgcccggcga gcgcgccggc gcgctcgttc agcgcggcgt
     4321 acgtgatcga gtccggcccg cacgcgaccg cctcggcgtc ggggcgggcg gcggcctgcg
     4381 cctcgaacat ctcgtggacg aggaggccgt ttccgtactc ggccgccgtc tcgttgaagc
     4441 cggcgagagt cacgcggtcc gcttccgtca gagcggtgag gtcgccgacc cttcgttcgg
     4501 gcgactcgac gatcgcccgc agcatcgtcg cgaacctgtc gagcagagcg gtcgcggccc
     4561 tctcgtcgac ccgtgcacgg tcgaacgaca acctgaacgt gaggccgtcc ccgggcgcga
     4621 tcaccatcga tatcgggtag ttcgtgttct ccgacgcagg cgctccctcg atccggacgt
     4681 cgccttcgtc ttcgtcccag acgtcgtcga gcgggtagtt ctcgaacgcg atcagcgtgt
     4741 cgaacagcgg cgtcggcgcg gtgatcgcac tctgcgcctg cacctcgacg agcggcgcgt
     4801 gctcgtggtc gcgacgctcg agcgcggcgg cctggagctt cttcagccac tccccgaccg
     4861 tctccgacgc ggggatgtgc gtgcgtatcg gaacggtgtt gatgaacagc ccggcggtcc
     4921 gctcgacgcg ctcgaccggc gcggaccggc ccgacatgat cgccccgaac aagacgtcgt
     4981 ccgaccccga gtagaccccg agggtcgcgg cccaggcggc ctcgacgacg gtgttcaagg
     5041 tcacgcgggc ggcgcgggcg aaagcggccg cgctctcggt ctccgcctcg ttgaccgtgc
     5101 gcacgaccat cccgtgacct tcgccgggcg tcgccggcgg gacgagccgc aggtccgtcg
     5161 gcgcttcgag gcccgcgagc gcctcccgcc agtacgccgc ggcggcagcg ggatcggcgg
     5221 cgcgcagcca ttcgacgaac gacttgtagt cgccgggcgg ctcgaggcgc gcagccggat
     5281 cgtcgtagag gtccatgagc tcgccgagca cgagctggac gctccatccg tcgagcagca
     5341 ggtggtggtg gctccacacc atccgccaag tggcgtcgtc gacgcggatc agcgagacgc
     5401 gcgtcaaagc ctcggtgagg tcgaagccgc gcgcgcggtc ctccgccagg aaccggtcga
     5461 ggtccgtgac ctcgttctgc gtcacctcga tgcgcggctc gggcgggacg agcatcatcg
     5521 gctcggggat cccctcccac tccaccgtcc ctgcgaggag cgggtggcgc gcggctacct
     5581 gctgccaggc gcggtcgagg cctgcggggt cgagggggcc ggtgatcgtc agcacgagct
     5641 gctcgaagta cagcgacccc gcggtcccca tcagggagtg gaacagcagg ccctgctgca
     5701 tgggcgacag cgggtacgcg tcggctacgc cgggtcgcga cgcgaccgcg tcgacgtcgg
     5761 cttcggtcgc ctgaccggtt gccgccgcga cggcttcctc cgccgccgcc acgaacgcgt
     5821 cgcgcagcgc ttcgatcgtc gaacggtcgt ggacttccga ggagtagacg aggtcgacgc
     5881 gcaccctccc ctcggcgacc caggcctcgg cctcgatcag gtggcggcgc gctccggccg
     5941 ggctgcgcag cgggccgacc gggccctcgg cgggctcgag gtcgccgctc ccgccgatgc
     6001 tcccttcgta gcggccgagg tagttgaagc tcacctcggg ctgtggggcg tcgcgcaggg
     6061 ggcctgcgtc ggcgggggtc ccgaggtagc gcagcaaccc gtagccgatg ccgccgccgg
     6121 gcgcggaccg catctggccg cggaccgact cgagggctgc gagagcgctc gagccgggct
     6181 cgaccacgac cgggaacagc gtcgtgaacc agccgacggt cctgctcagc tcgagccccg
     6241 acgtccccgc ctcgcggccg tggccctcga cgtcgacgac ggcgtgccgg ccatcggaga
     6301 tcgctccgag agctcgaccg aggatcgcga tcaaggcctc gttcggcagg acgtcgcggt
     6361 cgcgggcgat cgcgtcgacg agcgcgttgc tcgtggcctc gtcgagctcg aacgagacct
     6421 cggacgtcga gccgacgtcg ttgacgccgt cacggtcgcg ccgcaggagc gcgggctcga
     6481 gcccagacac gcggtcgcgc cacatggcct tctccgccgc gtctatctcc gcgacacggc
     6541 tctcgagatg gcgggcccac tcctggaacg acgtcgtttt cgcagggagc tcgaccgccc
     6601 tcccgcgcac cagctcgccg taggcgatcg agagatcgtg cacgagcacg ccccacgaga
     6661 tcgcgtcgac gacgaggtgg tggatcgtga gcagcagctg cgggggctgc agcggcccga
     6721 agtcgaacag cgcgccgcgc agcaagggcc cctgtgcgag gtcgaagctc gcctggagcc
     6781 gcgccgcctc ggcctccatc gccagcatcg ccgaggccgg gtcgcgtccg gacaggtcga
     6841 ccgtccacag gagctcgccg tcctcctccg gggcgatcac gccgcgccag ccggagccgt
     6901 cgcgcagcca gcgcgtgcgc agcacgtcgt ggtgctcggc gagcgcacgc agagcacctt
     6961 ccaacgcccg tgcgtcggcg ggcgcgcgca gctcgaacag catcgcctgg ttgaagtggt
     7021 gaggctcggc gaggtcctgc tcgaagaacc agcgctgaac cggcgtcagc gggaccgcgc
     7081 cggtgacgcg ttcgctcggc gcgcccggtg acggcttcga cgtctccacg acgcgagcga
     7141 ggtccgccac ggtctgggct tcgaagacgt gcttcggcgc gatcgcgatg ccgcgcgccg
     7201 ccgcccgggc cacgatctgg atgctcagga tcgagtcccc gccgaggtcg aagaagttgt
     7261 ccttgcgccc gacgcgatcg acgcgcagga cttcggccca gacctcggca agtgcggcct
     7321 ccgtctcggt ctccggcgcg tcgtaggtcg cttcgggccg ctcggggccc ggctccggga
     7381 gtgcggaacg gtcgagcttc ccgcccgggg tcacgggcaa cgcgtcgagg gtcacgaccg
     7441 cggccgggac catgtgggcg ggcaggacct cgccgagcga cgaccggacc tcgggcgtct
     7501 ccgcgtcccc gacgacgtac gcgacgagcc gcccggcgcg gaccgcgacg gccccgtcct
     7561 ccaccgaggg gagggcgagc agcgccgcct cgacctcggc gggctcgcgg cggtgtcccc
     7621 ggaccttcat ctgctcgtcg acacgtccga ggaagtcgac gttcccgtca cggcggatac
     7681 gcgctcggtc gccggtccgg tacatgcggg cgcccggctc cgccgagaac gggtcgggga
     7741 ggaaccgttc ggccgtcggc ccgggacgtc cgaggtagcc gcgagcgacg ccccggcccc
     7801 cgacgtagat ctcgcccggc aggccgcgcg gcagcgggtt caggtccgcg tcgaggacgt
     7861 acacacgtgc tccggtcgtg gcgcgtccga tgtgcgggac gccgtcgccg tcggatgtga
     7921 cttcgcctgc ggtgcagatc acggtcgcct cggcggggcc gtacaggtcg acgacgcgcg
     7981 ctccgtaatc gtgcgggggc cggaccgtga gcttgtcgcc gccgacgaac agcgtccgca
     8041 aggacgcggg gaccggacgc gcgagcacgg gggccgcgaa cacggtcgag acgaacgcag
     8101 ccgcgacctc gtgccgccgc cactcctcca ccagcgcgtc ggggtcgcgt cgggtgtcgt
     8161 cgtcgacgag cacgagagtc gccccgttcg cgagcgccga ccacagctcc cacaccgacg
     8221 cgtcgaacgt caacggcgcg acggtggaag cgcgcatgcc gggctccagc gggaacgccg
     8281 cggcatgcca gtcgacgagc tgcaggagat tcgagtgggt cacggcgacg cccttgggag
     8341 tcccggtcga gcccgacgtg tagatgacgt aggcgaggtc cgcggggtcg accgccgcgg
     8401 gctgcgccgg ctctcccgag ctcgacacgt ctacgtaggc gacgtcgccg agcgcggtcc
     8461 gcacctcctc cgcagccagg acggccgcag gactcgcgtc gcggatcatc agctgcaatc
     8521 gcgctgaggg ataggcggga tcgagcggga ggtacgcggc ccccgcgtac agcactccga
     8581 gggcggacac gacctgctcg aacgagcgcg ggtacgcaac cgccacgacg tcgccgcggc
     8641 cgacgccgcg cgaccgcagg gctccggcca cgactcccgc acgttcgtgc agctcgcccc
     8701 aggtaagcga cgcgtccccg aacagaaggg ctacctcgtc ggggtgcgcc tccgcccggg
     8761 cggctacacg ctcgtgcacg tacggccccg tcgagcgctc cggcagcggc gggttcggct
     8821 cttcgaccag cgccctggac tcgtccgggc cgaccagcgg gagccgcgac agcggccgcg
     8881 acgggtcggc gacgaccgcc tcgagcaata cctggaggtg ggcgatcatc gcctcgaccg
     8941 tgtcctcgtc gaagaggtcg gtggcgtact cgaggtaacc ctctagctca ccttcacccg
     9001 cgtcgccgat ccccagcgtc aggtcgaact tcgcagttcc cggcgcgatc ccgcggatgt
     9061 cgccctcgcc gaaggtcgag acggccaggc ccttcagcga gtagccctcc tgcggcgcgt
     9121 tctggtgcga gtacgcgacc tggaacagcg ggttgcgggc gaggtcgcgc ccctccccga
     9181 gcgccgacac cctcgtctcg aaggggacgt cgtcgtggtc gagcgcctgc gccgcgacgg
     9241 tcttcacgcg ctgcatcagg tcggtaaacg agggatcccc gccgaggtcg acgcgcagcg
     9301 gcagcatgtt gttgaagtag ccgatgagcc cgcgcgtctc ggccttcttg cgtcccgacg
     9361 tgggcgtccc gacgacgacg tcgtcctggc ccgaccagcg tgccatcagc acgtcgaacg
     9421 cggccatcat cgtcgtgaac atcgtcacgc cctcgcgccg gctcagcgca cgcaatccgt
     9481 ccgcgacgcg ctcgtcgatc cacagcgaga ccatcccgcc cgcgtagctc tgcaccgccg
     9541 gccgcggccg gtcggtcggg aggtcgagcg gaggcgctcc ggcgaggtgc gccgtccaga
     9601 agtcgaggcc cttctggagc gtctcgccgc tcatccactc ccgctgccac tgggcgaagt
     9661 ccgggtactg gatcggtagc accggcaggc gcgcgtcgcg gccttccttc gccgcgtcgt
     9721 acagcgccga cagctcgtcc tggatgatcg agggcgtcca tccgtcgtag acgatgtggt
     9781 ggaacgtcat cagcaggacg tggtcgccct cgccgagcgc gacgagcgtg ccgcggaaca
     9841 acgggggccg cgagaggtcg aacggcgtcg tcgcctcttc gtcgaacagg cgcagcgtct
     9901 ccgccctcgc ctcgtcgcgc ggcaacgagc tgaggtcgac gaacgggagc tcgacacggg
     9961 gctcgggctc gacgaacgcc cgaggctcgc cttcgacggc gacgaacccc atgcgcagca
    10021 cctcgtggcg gcgcgcgagg tcctccagcg ccttctccag cgcgggccgg tcgagctcgc
    10081 cttcgaggtg gaggtcgaac gggatgttgt agagcggcgt ccccggctcg agctggtcga
    10141 ggaaccagat ctgctcctgg ccgtaagaca ccgggaggta gccgccgcgc gggatcggga
    10201 cgatcggggg ctcgccgtcc gcggccccgg cggtcggcgc cagcgcgtcg atgcgagcgg
    10261 cgagctggtc gaggcggctg tgctcgtaga ggtcctggaa cgcgatctgc actccgaagt
    10321 cgcgcgccac gccgtcgagc acgaccatcc cggtgatcga cgtgccgccg aggtcgaagt
    10381 agtcggcgga cgcgtcgtcg ggctcgacct tcagcgcctc gttccagatg agcgcgagga
    10441 ccctctcggt gtccgtcgag aacgctccgg acggcgcgac ctccggtgac tcgaggagcg
    10501 cgcggacctc tcccggcgcg tggtcgtcgc cgcctcggcg gtagtccccc acgggccggc
    10561 accagcaccg ctcgcgctgg aacggatagg tcggcgcctc gatgcgcggc agctcgcggc
    10621 cctcgtagta acggtcccag tcgatcgcga cgccgcgcgc gtacagctcc gcgagcgcgt
    10681 gcagagcgga acggcccccg cgcagcagcg gcatcacgtc ggcctccggg cgcacggcgc
    10741 ggaccgaccg gccgaggacg ccgtcgccgc ccatctcgag gaagacgacg tcggagccga
    10801 gccgcgtgac cgcagccgcc agcttctcta cgtcgacctg cgccgacggc tgcacgaccg
    10861 cggcttcgcg cgctgcgtcc tcgacgtcca gcccggcgac gagcgtgcgc acggtgaggt
    10921 tgcctgcacc cgaaccgagg accgacgcct cggtgagccc cagcgaccgg atcaacgagt
    10981 gcagcgcctg gtgccagccg aacagtcgca acccgggggc cgcgccctcg ggcatctcga
    11041 ccgcgctgct caccggcgag aggtggtcga gcatggtctc gaaagcctgg cccgggattt
    11101 cctcgtcgcc cgagaacagc agcgccaggg gccgggcctc gcgcgggacg ggctgcagcg
    11161 actccgcgcc gaacgcggtc gcctccagcc acgacacgag ctcgtccttg ctcgccacga
    11221 cggccgccgc gcgcacctga tggtcgtccc gcgaccgatt gagcgtcgcc gcgaccgtcg
    11281 cgaggtcgtg ctcggtgtcg cgcacgaacg acgcgaacga gcggcagtag tcccgcagcg
    11341 cggcccccga cttcgcggag atcgtgacga gctccgcatc atcgcgagtc gagggggcag
    11401 gacgcgcggg agcttcctcc agcaccatgt ggacgttggt cccggcgagg ctgaacgagc
    11461 tgacgcctgc gcgccgcggc gacgccgcgg cccacggcgc cggctcgtcc tgcacccgga
    11521 cggcgtcctc gaagtcgatc agcgggctcg gcgactcgta gtgcagcgac gggaacagct
    11581 gcgcgtgctt cagcgacagg atcgccttca ccatcccggc gatccccgcc gcgtggtcga
    11641 ggtggccgac gttcgtcttg accgatccga cggggcacgg tgccgtgcgg ccactgcctc
    11701 ggaaggcctc gaccagacct tggacctcga tgacgtcgcc gagccgcgtc cccgacccgt
    11761 gcgcctcgac gtatccgatc gatgccggct cgacccgggc gttcgcccac gcggcgcgga
    11821 tcacctcggc ctgggcggcg gggctcggcg cgctgagccc gttcgaccgg tacccgttct
    11881 ggttcacggc gacgcccttg atcacggcgt gcacctggtc gccgtcctcg agcgcgtgtc
    11941 gcaacagctt cagcaccacg accgcgccgc cctcgcccgc ggcggtcccg tcggccgagg
    12001 cgtcgaacgc ccggcatttg ccgtcggggg acatgatctc ggtgaacgcg gcggtggtcg
    12061 tcgacggctc gaacaggacc gtcagcgcga ccccgccggc gatggcgaag cgcgcctcgc
    12121 ccgcgagcag gtcgcggcat gctgcgtcga ccgcgacgag cgagccgcaa caccccgcgt
    12181 ccatcacgag cgacggcccg tggagatcga ggacgtaaga gatgcgcccg gcgagcgcgc
    12241 tcggggcgtt gccgagcagc tccaaggggt cgacgtcgcg gaacagctcg tagtactcgg
    12301 gccgcggcgc gctgaggaac acgccggtgc gggtgccgcg cagggacgcg agcgagtagc
    12361 cggcgttctc gacggccgcg cacacgagct cgagcgtcac gcgctggtgc gggtccatgt
    12421 actccgcctc gcgcagggag atgttgaaga acttgtggtc gaagaggtcg acgcggtcga
    12481 ggcagccgac cgtcggatag tcgaggttcg ggtccacgct cgtggtctcg actcgctcgc
    12541 gcggcagcgc ccgcacgctg tcgcggcccc cggccaggtt ccgatacagc tccgccaggt
    12601 cccccgcttc ggggaagatc ccgctgatcc cgacgatcgc tacgtcttcg taggtcacag
    12661 ctcgaatccc gtcagtgccg gcgcggcggt ctcggcccgg gcgtcgatcg cggccgccat
    12721 cgcggtgacc gtcggatgct cgaagagctc ggctaccgag agcgcgcccg ggtagagctc
    12781 gtctgcgcgg ccgtcgagaa cgccggctac tcgctggcgt cgttgcgcgg cacccgcacc
    12841 ggcgtcttcc tcagcgcccc gcggcccgag tactacgagc tgttccgtga cgtcgacccg
    12901 ctcgagctgc tcggctacgc gccgagcgcg ctcgccg
//
LOCUS       DASQDO010000470         8311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_845316, whole genome shotgun sequence.
ACCESSION   DASQDO010000470 DASQDO010000000
VERSION     DASQDO010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845316"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..635)
                     /locus_tag="VEV43_12880"
     CDS             complement(258..635)
                     /locus_tag="VEV43_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24461.1"
                     /translation="MGRIRAMDHFYVGSVVIDVNDLDKMRAFWQEALGYVEDHSSDDW
                     VKLVDPKDKGVTVSLQLVPEPREGKNRLHLDLYAEDQMAEVERLEGLGAERERGPKED
                     EDFVVLSDPEGNLFCVIDQSATG"
     gene            complement(942..1301)
                     /locus_tag="VEV43_12885"
     CDS             complement(942..1301)
                     /locus_tag="VEV43_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24462.1"
                     /translation="MKRRLTLGVVAALAASVWAAPVASAQPQVICSANKVPPQTVVII
                     GGGEINIYPANGAPYALSVVGWARDVALCLVNSAAGPTLTCAKAYVNNRPTVTVDPNT
                     LQITISYEDFLQTACAV"
     gene            complement(1422..1640)
                     /locus_tag="VEV43_12890"
     CDS             complement(1422..1640)
                     /locus_tag="VEV43_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HYP24463.1"
                     /translation="MGIGASLFLIAIGAILAWAVEAELAGVDLQTIGVILLVVGVLGL
                     VMSMLFWSSFAPFRRTEERTVVRDRDVI"
     gene            complement(1723..3300)
                     /locus_tag="VEV43_12895"
     CDS             complement(1723..3300)
                     /locus_tag="VEV43_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYP24464.1"
                     /translation="MATSYDVVVVGAGHNGLTCAAYLARAGLRVVVLERTERIGGACV
                     TEEPWPGFRISTASYSLSLLEPGIVADLGLKLDVRIKDPELFAPFPDGGGLVLPVDPR
                     ARHEAIAGVSKKDADAYPRFVELFEEASRRLRPLLSYPATRKQARRAFRQSDVDKLFA
                     KTVDGSIADLCEEYFESELMQGLMASQGIIGSAAGPRTPGTAYVYLHHAFGLAAGEPG
                     AWGFVRGGMGAITAALADVVRAAGGEIRTEAPVERVKFEARRRVAGVVLESGEEIEAR
                     TVCSNADPKRTLSLLADAALPPELLEDVGLLPTEGTVVKVNCALGALPRFRGMGDAKE
                     PGPEHLGTVTISPSIAYLERACNDAAEGRPAREMFCEAWIQTASEPELAPAGKHTLSV
                     FAQYAPYELAEGTWEQRREEIGDLVLAALEQYAPGLTNLVEGRMVMGPPDLEARYGLT
                     GGNIFHGEILPDWLFDKRPVSTWHRHRWPVAGLYLCGSGAHPGGGVCGAPGRNAARAV
                     LEDLVASGDGLGTVPAT"
     gene            3397..3555
                     /locus_tag="VEV43_12900"
     CDS             3397..3555
                     /locus_tag="VEV43_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24465.1"
                     /translation="MALAGFCTVCQRTVYVDADSTPICPVCSSPLLEAAAPDDDSDDQ
                     ESVASAEK"
     gene            complement(3525..4130)
                     /locus_tag="VEV43_12905"
     CDS             complement(3525..4130)
                     /locus_tag="VEV43_12905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYP24466.1"
                     /translation="MPVTVHLIRHGQVENPKGVIYGRLPGYHLSERGVRQARGAAQHL
                     KDADLATIWASPLERAQETAAEIAAPHGLEIVTDDRLIESGNTFEGVGRTVWHLFSNP
                     AHWWRFRNPLKPSWGESFAEIRVRMMSAITEAAAGAEGREIAIVSHQTPVIVARLALA
                     KRRVPPWLGGAPCETGSVSTLVLDDGVVLSASYFSAEATDS"
     gene            4155..5240
                     /locus_tag="VEV43_12910"
     CDS             4155..5240
                     /locus_tag="VEV43_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HYP24467.1"
                     /translation="MPDILLFGATGYTGKITARALADRGADFVVAGRNRSKLEKLAEE
                     TGASAVRVVDATDVDGLTDALAGVKVLVTCVGPFLQLGHTAVEAALRAGVHYVDSSGE
                     GPFIERLIRDRHEDALHAGIAMAPAMGFDEVPGDVAVALAADGLELPELALTYAFPSQ
                     ASAGTLRSVLGIVPSKARWTVDGKTHRVRTAQETRWAPMPPPLGPQAAVSFPLASCHL
                     APLHAKLSSFRTYSTAGTWQRIGMKAAMPAVTALLSNDAVRSLATKAIDRLPEGPGDA
                     ARERGKWTILAEATTADGGWRNVVLTGTDVYGLTGKTLAAAAVTMAGRDYAERGVLAP
                     VQAAGLDTLRRELEDFGVRITRYEAKV"
     gene            5250..5645
                     /locus_tag="VEV43_12915"
     CDS             5250..5645
                     /locus_tag="VEV43_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24468.1"
                     /translation="MGARLQYAKVIDRELFMNRGGKLHPHLENQVLLHDEPGRAGVFL
                     VMRGWTDDHGTFTEQWRLEGPGGGVIYESLPRELHIATRAHVERLEDEVADLDFQYSA
                     DDYTLVFTLDDNEVARVNFPVTVQGDGSR"
     gene            5897..6235
                     /locus_tag="VEV43_12920"
     CDS             5897..6235
                     /locus_tag="VEV43_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24469.1"
                     /translation="MAVLRAGGAKVYEFAIVVLLGIGTFKLVDMVTEYVDLSKVVSIL
                     TLALGATVAWALEFSLFAQWEIPVRNELMAYIGTGLMISAAGYATPKVFEHVAEILGR
                     GTERPIRRAA"
     gene            6318..7616
                     /locus_tag="VEV43_12925"
     CDS             6318..7616
                     /locus_tag="VEV43_12925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYP24470.1"
                     /translation="MARLTELRRLERGMTRVRWFGAAFGALSIALQEGYPSRATEIAA
                     WALIGALAVGNLVIWGVQGRVDTVAAQRRLGDLAFAFDALVIGGIVWVFSFEDPYATW
                     ALLFVVPMEGALRYRFRGAVGAAAAVAVFFAAQSVHVADIRGEAFDLSTYVFVMCLSF
                     LIAGVTGSMAENWHAQSEALRVQGLRLAELDRLKDRFLAITSHEIRGPLTALIGGIDT
                     VRKRADTLTPEQKNGLLDMTAQQGRQLARMVDDLLLTSQLQGGNLKLHPHPCNLRETV
                     QGALDAAASKRRDHRLEVFVEPLTCVVDGARISQVVRNLVENAYKYTPKRTRVSVTAG
                     ADRGGITIEVEDDGPGIPAEKRDTMFHAFTRVEETAAGREGAGLGLFVVGQLVEAMGG
                     HIDVSSSSGGTCFAIHVPCETSELKSPRLSVVDPERSAGG"
     gene            7666..>8311
                     /locus_tag="VEV43_12930"
     CDS             7666..>8311
                     /locus_tag="VEV43_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYP24471.1"
                     /translation="MPSVTVDSRRIEYIDTGGAGTPVLLVHAFPLRAQMWEPQIESLG
                     DRWRFIAPDLMGFGASDGPDDASVYSVDSFADDLKAVLDELGIDRVVLAGLSLGGYVA
                     FAFLRKYRSSVSALVLADTRAEADAPENIEKRTNQQGLVREQGPGALTDGLVGALLGE
                     PTREKKPDVVANVRSLMENSPAGYIGALEAMKGRPDSTGDLASIDVPTLIVVGEN"
BASE COUNT         1275 a         2919 c         2879 g         1238 t
ORIGIN      
        1 gggcggccgt ggaaggtgct tccgaaggag cggccgaggc cgccgcgggc gggccctcgt
       61 cgtcgctgca cgcggccccc atgagggcga tcacgagggc gatgcccaga agcgtcccgc
      121 tccggcgggg acgagctcgc aacatgttct gcctccgatg actgggccgc tcgagcggcc
      181 gaccgcgcga tgagacgacg gccgacgccg tccggttgca ggggcaggct aagcgagcaa
      241 acccgccggc gcgcggatta gcccgtggcg ctctggtcga tgacgcagaa gaggttgccc
      301 tcggggtcgg acaggaccac gaagtcctcg tcctccttgg gcccccgctc gcgctctgcc
      361 ccgaggccct cgaggcgctc gacctcggcc atctggtcct cggcatagag gtcgaggtgg
      421 agccggttct tgccctcgcg cggctcgggg accagctgca gcgagaccgt cacgcccttg
      481 tccttggggt ccaccagctt gacccagtcg tcggacgaat ggtcctcgac gtacccgagg
      541 gcctcctgcc agaacgcacg catcttgtcg aggtcgttga cgtcgatcac gacggacccc
      601 acgtagaagt gatccatggc ccggattctt cccacgcggc cgaggttcac gcatgggcgg
      661 gtcgcctcgg cgagacgcct tcatgcatgg gtggggggcg gtgcgctcga gaccgtcccg
      721 cctaggattc ggctcgagga ttccgggcgg gcagcccggc tgcaccgccc ccacttccct
      781 tgcccagcgg tcgcgcaaga cgctcccgcg gtgcagccca ctgttcctta cgcatagtcg
      841 cgtcggcggt cacggtcgtg acaccggggt tgtcagactt gcgacagcgc acgcgaaagg
      901 ggcggcccct tgcggaggcc gccccttttt cttcgtttcg cctagaccgc gcaggcggtc
      961 tggaggaagt cctcgtagga gatcgtgatc tgcagcgtgt tcgggtcgac cgtcaccgtc
     1021 ggccggttgt tgacgtacgc cttcgcgcac gtgagcgtcg ggccggccgc ggagttcacg
     1081 aggcacagcg ctacgtctcg cgcccagccg accacggaca gggcataggg ggccccgttc
     1141 gccgggtaga tgttgatctc gccgccgccg atgatgacga ccgtctgcgg cggcaccttg
     1201 ttcgccgaac agatcacctg cggctgggcg ctcgccaccg gcgccgccca cacgctggcc
     1261 gcgagggccg cgaccactcc gagggtcaat ctccgcttca tgtcccactc cttcgtcttc
     1321 cgccgggtac gaggcggaga cttcgccatg gagggactcg taatccttcc ggcggcgcga
     1381 aaaaggggcg gccggtgtgg ccgccccttt ctgtgagctt gctagatcac gtcgcggtcg
     1441 cggacgaccg tgcgctcctc cgtgcggcgg aacggggcga agctgctcca gaaaagcatc
     1501 gacatgacga gcccgaggac tccgacgacg aggaggatca cgccgatcgt ctgcaggtcg
     1561 acgccggcga gctcggcctc gacggcccac gccaggatcg caccgatcgc gatcagaaag
     1621 aggcttgcac caatacccaa gttcgttcac ctccccgacg atgggcttct actggtggag
     1681 agggttaccc gaagcggaac gcaaccaaac cgctgccgag cgtcacgtag cggggacggt
     1741 gcccagtccg tccccggatg caaccaggtc ttcgagtacc gcgcgggctg cgttgcggcc
     1801 cggcgcgcca cagactccgc cacccgggtg cgcccctgag ccgcacaggt acagacccgc
     1861 taccggccag cggtggcggt gccacgtcga caccggccgc ttgtcgaaca gccagtccgg
     1921 caggatctcg ccgtggaaga tgttgccgcc ggtgagcccg tagcgggcct ccaggtcagg
     1981 cgggcccatc accatccggc cctcgacgag gttcgtgagt cccggcgcgt actgctcgag
     2041 cgcagccagc acgaggtcgc cgatctcctc gcggcgttgc tcccacgtcc cttccgcgag
     2101 ctcgtacggc gcgtactgcg cgaacaccga cagcgtgtgc ttgcccgcgg gcgcgagctc
     2161 cggctccgac gcggtctgta tccacgcctc gcagaacatc tcgcgcgccg ggcggccttc
     2221 ggccgcgtcg ttgcacgctc gttcgaggta ggcgatcgac ggcgagatcg tgaccgtgcc
     2281 caggtgctcg ggccccggct ccttcgcgtc gcccatcccg cggaagcgcg gcagcgcgcc
     2341 gagcgcgcag ttcaccttca cgacggtgcc ttcggtcggg agcaggccga cgtcctccaa
     2401 cagctcggga ggcaacgccg catcggccag caacgagagc gtcctcttcg ggtcggcgtt
     2461 cgagcaaacg gtacgcgcct cgatctcctc gcccgattcg agcacgactc ccgccacccg
     2521 tcggcgcgct tcgaacttga cccgctcgac cggcgcctcg gtgcggatct cacccccggc
     2581 agcgcgcacg acgtcggcga gcgcagccgt gatcgcgccc atgcccccgc gcacgaaccc
     2641 ccatgccccc ggctcccccg cggcgagccc gaacgcgtgg tgcaggtaga cgtacgcggt
     2701 gcccggggtg cgggggccgg ccgcggagcc gatgatcccc tgcgacgcca tcagcccctg
     2761 catgagctcg gactcgaagt actcctcgca gaggtcggcg atcgacccgt cgacggtctt
     2821 ggcgaagagc ttgtcgacgt cggactgacg aaaggcgcgg cgcgcctgct tgcgcgtcgc
     2881 cggatacgac agcaacggcc ggagccggcg cgacgcctcc tcgaacagct cgacgaagcg
     2941 cgggtacgcg tccgcgtcct tcttggagac gccggcgatc gcctcgtggc gcgcccgcgg
     3001 gtcgacggga aggaccagcc caccgccgtc ggggaacggg gcgaacagct cggggtcctt
     3061 gatgcgcacg tcgagcttca ggcccaggtc ggcgacgatg ccgggctcga gcaacgagag
     3121 cgagtacgac gcggtcgaga tgcggaaacc gggccacggc tcctcggtga cgcacgctcc
     3181 gccgatccgc tcggtgcgct cgaggacgac gacccggaga cctgcccggg caaggtacgc
     3241 ggcgcaggtg aggccgttgt gaccggcccc cacgaccacg acgtcgtacg acgttgccac
     3301 gtgcgcgcct tccttccttg gggtcccgcc gaggcggccc cgcgccccaa agattccctg
     3361 ttccactacc gacccgaggc aggctacctt tcccctatgg cgctagcggg cttctgcacc
     3421 gtctgtcaac gcactgtcta cgtcgacgcg gacagcacac cgatctgtcc ggtgtgctcg
     3481 tcgccgctgc tcgaagccgc cgcgcccgac gacgactcgg acgatcagga gtccgtggcc
     3541 tcggccgaga agtaggaagc cgacagcaca actccgtcgt ccagcacgag cgtcgacacc
     3601 gacccggtct cgcagggagc gcccccgagc cacggaggca ccctccgctt cgccagcgcg
     3661 agccgcgcga cgatcaccgg ggtctggtgc gacacgatgg cgatctcgcg gccctcggcc
     3721 cccgcggccg cttcggtgat cgccgacatc atccgcaccc ttatctcggc gaacgactcg
     3781 ccccacgacg gcttcagcgg attgcggaag cgccaccagt gcgccgggtt gctgaacaga
     3841 tgccacaccg tccggccgac gccctcgaac gtgttgccgc tctcgatcag ccggtcgtcg
     3901 gtcacgatct cgaggccgtg cggcgcggct atctccgcgg ccgtctcttg cgcgcgctcc
     3961 agcgggctcg cccagatcgt cgccaggtcc gcgtccttca ggtgctgcgc tgcgccgcgc
     4021 gcctgacgga ccccgcgctc ggacaggtgg tatccgggga ggcgcccgta gatgacgcct
     4081 ttcgggttct cgacctggcc atgacggatc aggtggaccg tgacgggcat ggcgacaagg
     4141 gtaggctgaa aaacatgccc gacatcctct tgttcggcgc tacggggtac acggggaaga
     4201 tcaccgctcg ggcgctcgcc gaccgcggcg cagacttcgt ggtggcgggg cggaaccgtt
     4261 cgaagctcga gaagctcgcc gaggagaccg gggcctccgc cgtccgcgtc gtcgacgcga
     4321 cggacgtgga cggcctcacg gatgctctcg ccggcgtcaa ggtgctcgtc acgtgcgtag
     4381 ggcccttcct ccagctcggg cacaccgcgg tcgaggcggc gctgcgcgcc ggcgtccact
     4441 acgtcgactc gtccggggag gggccgttca tcgagcggct gatccgcgac cgccacgagg
     4501 acgccctgca cgccgggatc gccatggcgc cggcgatggg cttcgacgag gtgccgggcg
     4561 acgtcgccgt cgcgctggcg gcggacggcc tcgagctccc cgagctcgcg ctcacctatg
     4621 cgttcccgag ccaggcgtcc gccgggacgc ttcgttcggt gctcgggatc gtcccgtcga
     4681 aggcgcgctg gaccgtcgac ggcaagacgc accgggtgag gaccgcgcag gagacgcgct
     4741 gggcccccat gcccccaccg ttggggccgc aggccgcggt ctcgttcccg ctcgcgagct
     4801 gccatctcgc gccgctgcac gcgaagctga gctcgttccg cacgtactcg accgccggga
     4861 cctggcagcg gatcgggatg aaggcggcga tgcccgcggt gaccgcactg ctgtccaacg
     4921 acgccgtgag gtcgctcgcg acgaaggcga tcgaccgcct gccggagggg ccgggcgacg
     4981 ccgcccgcga gcgcggcaag tggacgatcc tcgccgaggc cacgaccgcc gacggcgggt
     5041 ggcgcaacgt cgtcctcacc ggtaccgacg tctacgggct cacggggaag accctcgcgg
     5101 ccgcggccgt gacgatggcg ggccgcgact acgccgaacg cggagtgctc gcgccggtcc
     5161 aggccgcagg cctcgatacc ctgcgccgcg agctcgagga cttcggagtc cgcatcactc
     5221 gatacgaagc aaaggtctaa agggggccca tgggagcgcg actgcagtac gcgaaggtga
     5281 tcgaccggga gctgttcatg aaccgcggcg gcaagctgca cccgcacctc gagaaccagg
     5341 tgctcctgca cgacgagccg gggcgcgcgg gtgtgttcct cgtcatgcgc ggctggacgg
     5401 acgaccacgg caccttcacg gagcagtggc ggctcgaggg accgggcgga ggcgtgatct
     5461 acgagagcct gccgcgcgag ctgcacatcg cgaccagggc ccacgtggag cgtctggagg
     5521 acgaggtcgc ggacctcgac ttccagtact cggccgacga ttacacgctc gtcttcacgc
     5581 tcgacgacaa cgaggtcgca cgcgtgaact tcccggtcac cgtgcaggga gacgggtccc
     5641 gctagaaaag acgagggccg ccccgccgaa gcggagcggc ccgagaaaag agggcgggga
     5701 tctccgacgc gcgggcagtg cgtcggatgg ctgcggcggc gaatgcgact ggacccccgc
     5761 cctgcaggca gtgtccaccc tcgaccccct gcgagccaat agtccgtacg ggctatttcg
     5821 cgtcggcgaa tgatcgccca agggcaagct gtaacccgga cccactaccg gcgtccaacg
     5881 actcgcacag gacggaatgg ccgtcctgag agccggaggt gcaaaggtgt acgagttcgc
     5941 gatcgtggtt ctgctcggga tcgggacgtt caagctcgtc gacatggtga ccgagtacgt
     6001 cgacctctcg aaggtcgtat cgatcctcac gctggccctc ggggcgacgg tggcatgggc
     6061 gctcgagttc tcgctcttcg cccagtggga gatcccggtg cgcaacgagc tgatggccta
     6121 catcggcacg ggcctgatga tctcggccgc cggctacgcc acgccgaagg tcttcgagca
     6181 cgtcgccgag atcctcggcc gcggcacgga gcgtccgata cgccgggccg cgtaacggtc
     6241 ccaaccgaga caaaaagagg cccccggtcc gccgggggcc tctttcttcg cgctggattc
     6301 gcgccgagaa tctagacatg gccaggttga ccgagttgcg ccgtctcgaa cggggcatga
     6361 cgcgggtccg gtggttcggg gccgccttcg gcgcgctgtc gatcgcgctg caggagggct
     6421 acccgagccg ggcgaccgag atcgccgcgt gggctctcat cggcgcgctc gccgtcggca
     6481 acctcgtgat ctggggcgtc cagggccgag tggacacggt tgccgcccag cgccgcctcg
     6541 gagacctcgc gttcgcgttc gacgcgctcg tgatcggcgg gatcgtgtgg gtgttctcgt
     6601 tcgaggaccc ctacgcgacc tgggccctgc tgttcgtagt gccgatggag ggcgcgctcc
     6661 ggtaccgctt ccgaggggca gtgggggccg cagccgcggt ggcggtcttc ttcgcagccc
     6721 agtccgtcca cgtcgccgac atccggggag aggcgttcga cctctcgacc tacgtcttcg
     6781 tgatgtgcct gtcgttcctc atcgccggag tgaccgggtc gatggccgag aactggcacg
     6841 cgcagagcga ggccttgcgc gtgcagggtc tgcggctcgc ggagctcgac cggctgaagg
     6901 accgtttcct tgcgatcacg tcgcacgaga tccgcggccc cctcacggcg ctcatcggcg
     6961 ggatcgacac cgtccggaag cgcgccgaca cgctgacgcc cgagcagaag aacggcctgc
     7021 tcgacatgac ggcgcagcag ggacggcagc tcgcgcggat ggtggacgac ctgctgctca
     7081 cgtcgcagct ccagggcggc aacctcaagc tccacccgca tccgtgcaac ctgcgcgaga
     7141 ccgtccaggg agctctcgac gcggcggcgt cgaagaggcg ggatcaccgg ctcgaggtct
     7201 tcgtcgagcc gctcacctgc gtcgtcgacg gagcgcgcat ctcgcaggtc gtaaggaacc
     7261 tcgtcgagaa cgcgtacaag tacacgccga agcggactcg cgtctcggtt accgcggggg
     7321 ccgaccgggg cggcatcacg atcgaggtcg aggacgacgg ccccgggatc ccggccgaga
     7381 agcgcgacac gatgttccac gcgttcaccc gcgtggagga gacggctgcg ggacgcgagg
     7441 gggccgggct cgggctcttc gtggtcggtc agctggtcga ggcgatgggc ggccacatcg
     7501 acgtctcttc gtcgtcgggc gggacgtgct tcgcgatcca cgtcccgtgc gagacgagcg
     7561 agctgaagtc cccgcggctc tcggtcgtgg acccggagag gtcggcgggg ggctagcgac
     7621 tatcctcggg gccgactgag accgtctttc cgggaggccc acatcatgcc gagcgtcacc
     7681 gtcgactcca ggcgcatcga atacatcgac acgggaggcg cgggaacacc ggtgctcctc
     7741 gtgcacgcgt tccccctgcg cgcgcagatg tgggagcccc agatcgagtc gctcggcgac
     7801 cgctggcgct tcatcgcccc cgacctcatg ggcttcggag cgtccgacgg gcccgacgac
     7861 gcctcggtct actcggtcga ctcgttcgcc gacgacctca aggccgtgct cgacgagctc
     7921 ggcatcgacc gagtcgtgct tgccggtctc tcgctcggcg gctacgtcgc gttcgcgttc
     7981 ctgcgcaagt accgctcgtc ggtgtcagcg ctcgtgctcg ccgacacgcg ggccgaagcg
     8041 gacgcacccg agaacatcga gaagcgcacg aaccagcagg gcctggtgcg cgagcagggg
     8101 ccgggcgcgc tcacggacgg gttggtcggc gcgctgctcg gcgagccgac gcgcgagaag
     8161 aagcccgacg tcgtcgctaa cgtgcgatcg ctcatggaga actcccccgc cggctacatc
     8221 ggggcgctcg aggcgatgaa gggacgacca gactcgacgg gcgacctcgc gtcgatcgac
     8281 gtgccgacgc tgatcgtcgt cggcgagaac g
//
LOCUS       DASQDO010000471         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1212517, whole genome shotgun sequence.
ACCESSION   DASQDO010000471 DASQDO010000000
VERSION     DASQDO010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212517"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2592
                     /locus_tag="VEV43_12935"
     CDS             <1..2592
                     /locus_tag="VEV43_12935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HYP24472.1"
                     /translation="DDKIVQTAAKRQELVRAVGAYVAAKQRTGRIDFGDQVTKAVQVL
                     QTNPEIRDSYRRRYPFVLLDEYQDTNVAQRKLLQELVGPGGAVTAVGDARQAIFAWRG
                     ATMYNLIGFPYDFPRSDGVVYDPVSLSENFRSGRRILEVANRVVDPIDASRRPGDPLR
                     AHGANGEGRVEVGLFTDERAEAEWIASRCEELHGQVLAEGRDPVAWKDVAILVRRKAA
                     MDMLLQTLEEHEIPVEVVGLGGLLKTPEVTEIVAWLRALETKPGANRWLGRILLGPRW
                     RIHYRDLSLLARWAAARNFDLKVQLAGGDEEAARDVEPGDVGFSLTEALAHVDEIEDL
                     GAEAKRRLRAFSERLAALRKKSNASLLELVQEVIRQAGIVDALDSSSSRAAPSAKQNV
                     SNFLDQVASFAPVEGEASLRSFIAYLDAAEDAEETLEATQPADQDSVKLMTVHTAKGL
                     EFECVFVPSVAAAVGRTGDPVFSIFPNTRASNPLTSYSELPYEVREDSEHLPKFEGKL
                     GDFQKAVRDRVLEDERRLFYVSLTRAKQNLAVTASWWYGRDKQPKGPSPFWNELAELT
                     SDRTGDAPGAGDLVDCVSRADAPDENPLFRAMEDRRVWPPAPRIGIEDELFPEGWGAA
                     ADAVVAGETTVESLLSELPGDERAEAEQLVASFEQDLQVIAAAVAPRRPAEPRVPEFV
                     SATSQVRLGKGEIAAWDLVRPLPDRPTRARRLGTEVHRMIEERSRGMAAFPDESELDE
                     PGRVDAGDVRDGLLSHWEASGYGDRALAVLPSGEPMVELPFTLKRDGRFVRGRIDAVY
                     EAPGGIEIVDFKTGRRFEVDTEHDQLALYAEALDANGLIPEGAEVTLSYVFLDGEPPV
                     SRVWARA"
     gene            2619..>4990
                     /locus_tag="VEV43_12940"
     CDS             2619..>4990
                     /locus_tag="VEV43_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYP24473.1"
                     /translation="MTKTDVLARFSAPTKAWFESAFDAPTAAQVGAWDAISGGRDALV
                     VAPTGSGKTLAAFLWSIDRLSQPRPADAAPGVRVLYVSPLKALAVDIERNLRSPLSGI
                     RGAAVRLELPPPEISVSVRTGDTPQEDRRRFASKPSDVLITTPESLFLLLTSAARETL
                     RTVETVIVDEVHAVAGTKRGSHLALSLERLDELLDKPAQRVGLSATVRPHDEVARWLG
                     GPRAVDVVAVPSEKEFDLSVVVPVEDMSEMGEPLDEGVGGERQGNVSGPEAKNSVWPH
                     VEAALLDLVKAHRSTIVFANSRRLAERLCSRLNELADELIARAHHGSVSREQRLLIED
                     DLKSGRLPAVVATSSLELGIDMGAVDLVVQVEAPDSVASGLQRVGRAGHQVGAVSRGV
                     FFPKFRGDLLECAVVIQRMREGAIEELRYPRNPLDVLAQQIVAMVAMEPWEVDALEAV
                     VKRAAPFADLPRAGLESVLDMLSGRYPSSDFAELRPRLNWDRSSNELTPRGNAQHLAV
                     TSGGTIPDRGLFGVFLVGEKAARVGELDEEMVYESRPGDVFLLGASSWMIEEITHDRV
                     IVSPAPGAPGKMPFWHGDAPGRPVELGRALGEFVREVSAAKPEEQKRRLEGAGLDAFA
                     TSNLVSYLKEQREATGALPDDRTIVIERFRDELGDWRVCIHSYFGARVHAPWSQAIEA
                     RFRERLGLEVQCMYTDDGIVVRVPEADDAPPGESVLFEPEEIEDVVLGQVGGSALFAS
                     RFRECAARSLLLPKRRPGTRTPLWQQRQKSAVLLQAAAKHESFPVILETYR"
BASE COUNT          716 a         1667 c         1892 g          715 t
ORIGIN      
        1 gacgacaaga tcgtgcagac cgccgcgaaa cggcaggagc tcgtgcgcgc ggtcggcgcg
       61 tacgtcgccg cgaagcagag gacggggcgg atcgacttcg gggaccaggt cacgaaggcc
      121 gtccaggtcc tgcagacgaa ccccgagatc cgcgactcgt accggcgcag gtatccgttc
      181 gtgctcctcg acgagtacca ggacacgaac gtcgcacaac gcaagctgtt gcaggagctc
      241 gtggggcccg gcggcgcggt gacggccgtc ggagacgcac gccaagcgat cttcgcctgg
      301 cgcggggcga cgatgtacaa cctcatcggc ttcccctacg acttcccgcg ctccgacggc
      361 gtcgtgtacg accccgtgtc gctgtcggag aacttccgct cgggccggcg gatcctcgag
      421 gtagcgaacc gggtcgtcga cccgatcgac gcatcgcggc ggccgggcga ccccctgcgg
      481 gcgcacggcg cgaacggcga gggccgggtc gaagtcgggc tgttcaccga cgaacgtgcc
      541 gaggccgagt ggatcgcgtc acgctgcgag gagctgcacg gccaggtgct cgcggagggg
      601 cgcgaccccg tggcttggaa ggacgtagcg atcctcgtgc ggcgcaaggc cgcgatggac
      661 atgctgctcc agaccctcga ggagcacgag atccccgtcg aggtcgtggg gctcggcggt
      721 ctgctcaaga cccccgaggt gaccgagatc gtggcgtggc tgcgggcgct cgagaccaag
      781 ccgggcgcga accggtggct ggggcggatc ctgctcggcc cccgctggcg catccactac
      841 cgcgacctct cgctgctcgc gcggtgggcc gcggcgcgca acttcgacct caaggttcag
      901 ctcgccggtg gcgatgagga agccgctcgc gacgtcgagc ctggagacgt cggcttctcg
      961 ttgaccgagg cgctggccca cgtggacgag atcgaggact tgggggccga ggcgaagcgg
     1021 cggctgcggg cattctcgga aaggctcgcg gcgctgcgca agaagtcgaa cgcgtcgctg
     1081 ctcgagctcg tccaggaggt catccgtcag gccgggatcg tcgacgcgct cgactcgtcg
     1141 tcgtcacggg cggcgccgag cgcgaagcag aacgtctcga acttcctcga ccaggtggcg
     1201 tcgttcgcgc cggtcgaggg tgaggcgtcg ctgcggagct tcatcgccta tctcgacgcg
     1261 gccgaggacg ccgaggagac gctcgaagcg acgcagcccg cggaccagga ctcggtcaag
     1321 ctcatgacgg tccacaccgc gaaggggctc gagttcgagt gcgtgttcgt cccgtcggtc
     1381 gcggccgccg tcggcaggac gggcgatccc gtgttctcga tcttcccgaa cacgcgggcg
     1441 tcgaacccgc tgacgagcta ctccgagctc ccgtacgagg tgcgtgagga ctcggagcac
     1501 ctgccgaagt tcgagggcaa gctcggcgac ttccagaagg ccgtgcgcga ccgtgtgctc
     1561 gaagacgagc ggcggctgtt ctacgtgtcg ctcacgcggg cgaagcagaa cctcgcggtc
     1621 acggcgtcgt ggtggtacgg gcgcgacaag cagccgaagg ggccgtcgcc gttctggaac
     1681 gagctcgccg agctcacgag cgatcggacc ggcgatgctc ccggagcggg cgacctcgtc
     1741 gactgcgtgt cgcgagccga cgcccccgac gagaacccgc tgttccgggc gatggaggac
     1801 cgccgggtgt ggccgcccgc tccgcggatc gggatcgagg acgagctgtt ccccgaggga
     1861 tggggggccg cggccgacgc ggtggtcgcg ggcgagacca ccgtcgagtc gctcctgtcc
     1921 gagctgccgg gggacgagcg ggctgaggcc gagcagctcg tcgcttcctt cgagcaggac
     1981 ctccaggtga tcgccgcggc cgtcgctccg cggcggcccg ccgagccgcg ggttcccgag
     2041 ttcgtctcgg cgacgtcgca ggtccggctc ggcaagggtg agatcgctgc ctgggacctc
     2101 gtgcggcctt tgccggaccg gccgacgagg gcacgccggc tcgggacgga ggtgcaccgg
     2161 atgatcgagg agcgttcccg ggggatggct gcgttccccg acgagagcga gctggacgag
     2221 cccggccggg tcgacgccgg cgacgtgcgc gacggcctgc tgtcgcattg ggaggcgtcg
     2281 ggatacggcg accgggcgct cgcggtgctg ccgtcgggcg agccgatggt cgagctcccg
     2341 ttcacgctga agcgggacgg gaggttcgtc cggggccgga tcgacgcggt gtacgaagct
     2401 cccggcggga tcgagatcgt ggacttcaag accgggcggc ggttcgaggt ggacacagag
     2461 cacgaccagc tcgcgctgta cgccgaggcg ctcgacgcca acgggctcat ccccgagggg
     2521 gccgaggtga cgctgagcta cgtcttcctc gacggcgagc cgcccgtgtc gagggtctgg
     2581 gcccgcgcct agggggccgc gcccggcatg atggacgggt gacgaagacg gacgtgctgg
     2641 cgaggttctc ggcccccacg aaagcgtggt tcgaatccgc gttcgacgcg cccacggccg
     2701 cccaggtggg tgcgtgggat gcgatcagcg ggggccggga cgcgctcgtg gtggccccga
     2761 cggggtcggg caagacgctc gccgcgttcc tgtggtcgat cgaccggctg tcgcagcccc
     2821 ggcccgccga tgcggcgccg ggtgtgcgcg tgctgtacgt gtccccgctc aaagcgcttg
     2881 cggtcgacat cgaacggaac ctgcgctcgc cgttgagtgg gatcaggggg gccgcggtgc
     2941 ggctcgagtt gccgccgccc gagatctcgg tctcggtgcg gaccggcgac acgccgcagg
     3001 aggaccgccg gcgcttcgcc tcgaagccga gcgacgtcct gatcacgacg ccggagtcgc
     3061 tcttcctcct gctgacgtcc gcggcgcgcg agaccttgcg cacggtcgag accgtgatcg
     3121 tcgacgaggt ccacgcagtc gccgggacga agcgcggctc gcaccttgcc ttgagcctcg
     3181 agcgattgga cgagctcctc gacaagccgg cgcagcgtgt cgggctgagc gcgaccgtcc
     3241 gcccgcacga cgaggtcgcg cgctggctcg gggggccgcg ggccgtcgac gtcgtcgcgg
     3301 tcccgagcga gaaggagttc gacctctccg tcgtcgtgcc ggtcgaggac atgtccgaga
     3361 tgggcgagcc gctggacgag ggggtgggcg gggagcggca ggggaacgtc agcgggccgg
     3421 aggcgaagaa cagcgtgtgg ccgcacgtcg aggccgcgct gctcgacctc gtcaaggcgc
     3481 atcgctcgac gatcgtgttc gcgaactccc ggcggctcgc ggaacggctg tgctcgcgtc
     3541 tgaacgagct cgccgacgag ctcatcgccc gggcgcacca cggaagcgtg agccgcgagc
     3601 agcgtctcct gatcgaagac gacctcaagt ccggccggct gccggccgtc gtggcgacga
     3661 gctcgttgga gctcggcatc gacatgggcg cggtcgacct cgtcgtccag gtcgaggctc
     3721 cggactccgt cgcgtcgggc ctccagcgcg tcgggcgtgc cggccaccag gtcggcgccg
     3781 tcagccgggg cgtgttcttc ccgaagttcc gcggcgacct cctcgagtgc gccgtggtca
     3841 tccagcggat gcgcgagggg gcgatcgagg agcttcgcta cccgcgcaac ccgctcgacg
     3901 tgctcgcgca gcagatcgtc gcgatggtcg ccatggagcc gtgggaagta gacgcgctcg
     3961 aagccgtcgt gaagcgcgcg gcccccttcg ccgacctccc gcgggccggt ctcgagagcg
     4021 tcctcgacat gctctcgggc cgctacccgt cgagcgactt cgccgagctg cggccgcggc
     4081 tcaactggga ccggtcgtcg aacgagctca ccccgcgcgg gaacgcgcag cacctcgccg
     4141 tgacgtcggg tgggacgatc ccggaccgcg ggctgttcgg cgtgttcctc gtcggcgaga
     4201 aggcggcgcg cgtcggcgag ctcgacgagg agatggtcta cgagagccgt cccggcgacg
     4261 tcttcctgct cggcgcgtcg agctggatga tcgaagagat cacgcacgac cgcgtgatcg
     4321 tctcgcccgc cccgggcgct cccgggaaga tgccgttctg gcacggggac gcgccgggcc
     4381 gtccggtcga gctggggcgc gcgctcggag agttcgtccg cgaggtctcg gctgccaagc
     4441 ccgaggagca gaagcgtcgc ttggaggggg ccgggctcga cgcgttcgcg acgtcgaacc
     4501 tcgtctcgta cctgaaggag cagcgcgagg cgacgggcgc gctgcccgac gaccgcacga
     4561 tcgtgatcga gcggttccgc gacgagctcg gcgactggcg ggtgtgcatc cactcgtact
     4621 ttggcgcgcg cgtgcacgcg ccgtggtcgc aggcgatcga ggcgcgcttc cgcgagcggc
     4681 tcggcctcga ggtccagtgc atgtacaccg acgacgggat cgtcgtgcga gtgccggagg
     4741 ccgacgacgc gccgccgggc gaatccgtgc tgttcgagcc cgaggagata gaggacgtcg
     4801 tgctcggcca ggtcggcggc tcggccctct tcgcgagccg gttccgcgag tgcgcggccc
     4861 ggtccttgct cctgccgaag aggcggcccg ggacccggac cccgctgtgg cagcagcgtc
     4921 agaagagcgc ggtgctgttg caggccgcgg cgaagcacga gtcgttcccc gtgatcctcg
     4981 agacctatcg
//
LOCUS       DASQDO010000472         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_295540, whole genome shotgun sequence.
ACCESSION   DASQDO010000472 DASQDO010000000
VERSION     DASQDO010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295540"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..721)
                     /locus_tag="VEV43_12945"
     CDS             complement(89..721)
                     /locus_tag="VEV43_12945"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYP24474.1"
                     /translation="MKEQGPAGRRDRREDILAASLHLFAEKGFHGTSMRDIAREADIT
                     EGLIYHYFSGKRDLFRAIIEERSFLPLLRTLPELAAQLDLRALLIVLARGFFDVLREN
                     KELTRLLLQEVQVFPEEKEAFFSDFFSESIEQLAQVFDGRMNERARGQVDPQMAARLF
                     FNSLLAFFVEQEILGGKQVLPADEGSYIEHLGEMFVKRMGPGRPKPSLVR"
     gene            complement(759..1085)
                     /locus_tag="VEV43_12950"
     CDS             complement(759..1085)
                     /locus_tag="VEV43_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HYP24475.1"
                     /translation="MRVTVSAPGEDLKPADVDAITADLEKIGRRLNGFSEEVVANVRV
                     SHAQNAQRVPGHHVVLEVHYGPNHLIAKTENPDMGMAVREAREEILRQINDRSTGGHS
                     SQAKGR"
     gene            complement(1115..>1543)
                     /locus_tag="VEV43_12955"
     CDS             complement(1115..>1543)
                     /locus_tag="VEV43_12955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP24476.1"
                     /translation="GTGPEEKSLRRAAARIPQRVVFAGRVPDADAPALYAAADVFALP
                     VADRWRGLDTEGLGVVLLEAAACEVPCVTGRSGGTPEAVVDGVTGFVVDAREERRLTD
                     AIVRLLEDRDLARKMGRAGRRHVADEFGGRVPEPLTQWLE"
BASE COUNT          220 a          559 c          517 g          247 t
ORIGIN      
        1 gtccgtcgct acgaggccga cacgaagggc cgcacgacgg tcctcgacaa ggtcgacgcg
       61 ctgatctagc aggtcttgcc gggccgccct accgtacgag cgacggcttg gggcggcccg
      121 gccccatcct cttcacgaac atctccccga ggtgctcgat gtacgagccc tcgtcggccg
      181 gcaggacctg cttgccgccg aggatctcct gctcgacgaa gaacgccagc agcgagttga
      241 agaacagccg cgcggccatc tgagggtcga cctgaccccg cgccctctcg ttcatgcgcc
      301 cgtcgaacac ctgcgcgagc tgctcgatgg actcgctgaa gaagtcggag aagaacgcct
      361 ccttctcctc cgggaacacc tggacctcct gcaggaggag ccgcgtcagc tccttgttct
      421 cgcgcagcac gtcgaagaac ccgcgcgcca gcacgatcag cagcgcgcgc aggtcgagct
      481 gcgcggctag ctcgggaagc gtgcgcagca acggcaggaa cgaacgctcc tcgatgatcg
      541 cccggaagag atcgcgcttg cccgagaagt agtggtagat caggccttcc gtgatgtccg
      601 cctcgcgcgc gatgtcgcgc atcgacgtgc cgtggaagcc cttctcggcg aacaggtgca
      661 ggctcgccgc gaggatgtcc tcgcggcggt cgcgccgtcc cgcggggccc tgctccttca
      721 tgcccccggc gtccggccga ggccgcccgt cgccgtgact accttccctt cgcctgcgag
      781 ctgtgaccgc cggtcgagcg gtcgtttatc tggcggagga tctcctctcg cgcctcgcgc
      841 acggccatcc ccatgtcggg gttctcggtc ttcgcgatga ggtggttggg accgtagtgg
      901 acctcgagca cgacgtggtg ccccggcacg cgctgcgcgt tctgcgcgtg gctcacgcgt
      961 acgttcgcga ccacctcttc gctgaagccg ttgagccggc ggccgatctt ctcgaggtcc
     1021 gcggtgatgg cgtcgacgtc ggccggcttc aggtcctccc cgggggcgga cacggtgacg
     1081 cgcatggatt cctcctagtc gttggttgcc ttgactactc tagccattgg gtcagcggct
     1141 ccggtacccg tcccccgaac tcgtccgcca catgcctgcg acccgcgcgg cccatcttcc
     1201 gggcgaggtc gcggtcctcg aggagccgga cgatcgcatc agtcagacgc cgctcctccc
     1261 gcgcgtcgac gacgaacccc gtgacgccgt cgacgacggc ctcgggcgtg cctccggaac
     1321 gtcccgtcac gcaggggacc tcgcaggcgg cggcctcgag caggacgact ccgaggccct
     1381 ccgtgtcgag ccctcgccag cggtcggcga ccgggagcgc gaagacgtcc gcggcggcgt
     1441 agagggccgg ggcatcggcg tcggggacgc gaccggcgaa gaccacgcgc tgcgggatgc
     1501 gtgcggccgc gcgccggagc gacttctcct cgggccccgt ccc
//
LOCUS       DASQDO010000473        11791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_49718, whole genome shotgun sequence.
ACCESSION   DASQDO010000473 DASQDO010000000
VERSION     DASQDO010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49718"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..810)
                     /locus_tag="VEV43_12960"
     CDS             complement(19..810)
                     /locus_tag="VEV43_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24477.1"
                     /translation="MDELRRPLFVLAIVLMAIVVLFEIGSAALVKGVAPAAGQLRDIA
                     LEEANRDPDDELEPEDVTDGDVVAQLEELSRDEDPPGRAIQYMAFLDGLLLYAVGMMG
                     LSLVAPERIQGRVQGIVGLILSILLVIGLIIAIVFAVVLLMIMVGLLTAVPFGTIAYF
                     AIYGFFDRTGASVVLGLIMLLKLGFCVCLVLAQQRFLQNKSIVLLTLTSLLASVIVAF
                     LHGIVPLFLVSITDGIAAIVIAILAIIWAIVMLIASIVSIVKLIL"
     gene            complement(846..1184)
                     /locus_tag="VEV43_12965"
     CDS             complement(846..1184)
                     /locus_tag="VEV43_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24478.1"
                     /translation="MPLSDGTAFLFGCIGGALPDVVKLIQSKTEMPTYLKKPWWYVVF
                     VITVALGGLVAGFLLEPNTRVDAVAYGFSALEIIRRIGGSALGGNGGTPGTRTTDLAA
                     TPPRVRDYLA"
     gene            complement(1227..1676)
                     /locus_tag="VEV43_12970"
     CDS             complement(1227..1676)
                     /locus_tag="VEV43_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24479.1"
                     /translation="MKVVIVVLVVVLLLFVAALGIGAGQDRGDGDAGRDGIAAWLADR
                     FLRPVPAVEPDELSAGCRQGSVLLVPAGGSCTVGVAESSRFSRELRLVATAGAARLAL
                     ARDEGLDVEVDVAPGDDARAEIDLGRDEATVAVICTAVEACVLEVAS"
     gene            complement(1690..2667)
                     /locus_tag="VEV43_12975"
     CDS             complement(1690..2667)
                     /locus_tag="VEV43_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24480.1"
                     /translation="MAKWWAITGPAKTELTNGVGEAAFTVTNVTATAVRGLVRVMPEG
                     TAKPEMFKVVGESTLDWAPNETKQVTVQINGQGAPPGSYVFTFAVVSEADPSELSDRL
                     SSAFDVGAAPAAKKFPIGILVGIVAGVLVIGGVLFWLLSRGDDGTPAAATTASPAAES
                     PPPAETPTPAPPEPPRADCLGYDPNALNLVPEPGGYLITDGGSRMLFLTDPTNANNAY
                     RVARSFSQHCFIGRSNPRPNRRDYIVEYWLDPSGIATPPEDACTTYDRGALQVVDQGA
                     QGFVVTDGGGLQVLVDNEQDAQLARQLARSFTRRCFIAEGTPDEVEYWL"
     gene            complement(2660..3226)
                     /locus_tag="VEV43_12980"
     CDS             complement(2660..3226)
                     /locus_tag="VEV43_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYP24481.1"
                     /translation="MRQAEIRRLLPSVFERTIDGGGPLGPVLDVMEHLHEPAERVLVE
                     LDAYLDPMRTRDDGVPMLARWLDLDWLMVANPEDALPSDFAPLASGTGRMRALIGSAA
                     WLSKLRGTVDGLTRFLEIATGVRGFAVYENVTADSTAHPFHLLVSVPAAARGYEAMVR
                     RILEAQKPAYATYELSMQPGQEEEVTDG"
     gene            complement(3213..5792)
                     /locus_tag="VEV43_12985"
     CDS             complement(3213..5792)
                     /locus_tag="VEV43_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HYP24482.1"
                     /translation="MTLPTPNLDDRDFEDLLEQARTRIVQLSPDWNDLTPSDPGMVLL
                     ELFAYLTDTMIYRLNRVPDKAYVAFLNLLGVRLMPPGAASVMLTFSRKQASETALEIP
                     RGTEVSAARATTGGTPPVFVTADAATIPPGKTEVDVLAHHCTLAEAELVATGTGLPGL
                     TVAVGHPPIVAPTGHPLDLLVGVEAAADELDERAPAIQHDGRPFRIWSEVGSFANAGD
                     DPHVFHVDRVAGRISFAPAVRGADEGGALEDVPVALAGVPKAGREIRVWYRSGGGPDG
                     NLGAETLTTMKSPIPGVEVTNRSPATGGRPAETLDNALIRGPQSLHSLERAVTARDFQ
                     LVALHSSGAVARARAFTKANLWRHALPGTVSLLMVPYVPNDLRVGGALTTGTLAEHQT
                     DNALLEVSAALERRRPAATLVEVGWARYKSVSVRARVVVHREEDLAAVERRVRERLHA
                     TINPLPTQGTRGWRFGEALRVSNVYDRPLAEAGVRYVDRVRLLVDEVPDEEVLSVAPD
                     HFQPSTWYAGSRTTVFRSLNDGEGWEVAARLPGKSVEVVRAHPQVPGLVAAISRLDNE
                     ASHLQISVDCGETWQEGVQLGFGVEDVAWSMRDQTPLLFLATDKGLYELTPTRAGATP
                     LQVVVDAANPDRGYYSVAVAADQAGRVNVALAAQDAAGTFLSSDGGRSGSFRSIGLNG
                     EDVRVLAVQQDGPRAFLWAGTASVGSDDPGKGCYSWELRGEDNPPEGWVPRQVGWRGG
                     TCWSIAFRGGLVLAATHSAGVLRLDSGSLNPQWIAADVRCGLPLRDPGRFHPVRSLAT
                     DPTATRLLTAGPEGLCRSDDFGVAYQFVSAAEFTERVTLPPTWLFSSGEHELEMVSEL
                     AAS"
     gene            complement(5789..6223)
                     /locus_tag="VEV43_12990"
     CDS             complement(5789..6223)
                     /locus_tag="VEV43_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HYP24483.1"
                     /translation="MNERARFRALRFVHPDVAGDAAGGLRLSPRVGFEMVAEDDSIRQ
                     AILLLLSTIPGERVMRPTYGCLLHRVVFSPNDDTTAGLAIHYVRQALEWWEPRIDLLD
                     VDARRNPTDPGRLDLTLVYRVKATLKLDRMELPVQLSGEEVA"
     gene            complement(6220..6561)
                     /locus_tag="VEV43_12995"
     CDS             complement(6220..6561)
                     /locus_tag="VEV43_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24484.1"
                     /translation="MLWLTEDAKLVCKHQAGIVSNAPSQPWVTIQGRRVLVSTDPEGR
                     PINGCPNINVGVRPCKTTLAVKQGYSEWLRVDGDRVCLDSVQGLTDGTPAGFVKYVVE
                     QPGQAFVSEAP"
     gene            complement(6576..8102)
                     /locus_tag="VEV43_13000"
     CDS             complement(6576..8102)
                     /locus_tag="VEV43_13000"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HYP24485.1"
                     /translation="MTRVLTLPRVAVSLGGGELPLELGETLVGLSVRHELALPAQCEA
                     TFHDHWRASVDDRAIEPGTPFEVAVDDATLFSGEVTAIERVYAADGNKRVRVRGYDVL
                     HRLKKTQPVRTFKDSDAAALASELVRPLGLDVSAAHDGPVWPLLVQYRQSDLELLVDV
                     TRRCGLYPVVLDGRLELVDLRGRGEPVRLVLGESALELRITVNADAAAGGVAATGWDT
                     STVELRTGAAAEAGSGRVVAASVPASSVGADGDRSLVDESTSTDAQADALAQAELDRR
                     VAAEVTLWAAAPGHADLRPATVVDVSGVADSVSGRYVVTSVVHTVDQDNGFVTEISTD
                     PPRARLRPRGASVTVGVVADVDDPGSIGRVRLRLPAHGDVETEWIPVVMAAGGPSKGL
                     IALPDVDDHVLVVLAHEDPARAFVLGGLYGADGPPDAGVVDGDVRRFTFLTAGGHKVV
                     LDDEAVAVRLEDATGSFLEMSPSAVRLHSEANLQIDAPGKAVVVRGNTVDFMRAEEPD
                     "
     gene            complement(8099..8827)
                     /locus_tag="VEV43_13005"
     CDS             complement(8099..8827)
                     /locus_tag="VEV43_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24486.1"
                     /translation="MERVAFLVEETGERLSALLNPESLVMKRRAGLRSRRGVSGQLTG
                     TRLMDDPLLYTGGGATELQLDLLFDTFLAGEGASEDVRDLTRPLWQLAENARSEGAYG
                     RPPLVRFLWGKVWNYPGIVQAVAERVEQFAASGAPRRSWLRMRLLRSAEPPPPTAAAP
                     GPGTLVLPRGGEVPPDNVMVHEVAGAGPDAPGERLDAIAERYYGNASLWRVIATFNGI
                     GDPLHVPPGTLLRIPPLSVFASAS"
     gene            complement(8827..9810)
                     /locus_tag="VEV43_13010"
     CDS             complement(8827..9810)
                     /locus_tag="VEV43_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24487.1"
                     /translation="MLRKLTGRVVAGLAAVARAVAGVLDGWARRWGGPEHPAGGPPAD
                     WAARVRAKAPWLLEPEAEDVLRHELPPPFGPAGRPSAATAGEPARPGRLRSSLAAAPR
                     PAVQEAAIEPDAPARPRPPVPAIVTRARARVADGITLTTKVVAAVRAEVERTPARDGR
                     RRDAEPPAPDAPPPRPDADVVPDEPAPEPPEKKAAPPGRVGFATRPRRSAIETTRERR
                     AAAFPRLADAFAMHPSGKASPSSVPASLEPRWVQAFEPEETAVTAEIGIAAGWPDVPD
                     DPSVAPAATLHDDEGSWPELPPGISDDEDVPQQALLERDRRASLDSELRSL"
     gene            complement(9817..10296)
                     /locus_tag="VEV43_13015"
     CDS             complement(9817..10296)
                     /locus_tag="VEV43_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYP24488.1"
                     /translation="MPEAAPTAAPAASGAQPGTFVDPYRSYNFKLSIGGVVEGHFVEC
                     RGLEVKIQPIRYREGGTQQVVHAIPGRVDYGTVELRYGLTQSRDLWQWFMTGVQGKVE
                     RRNVSILLLDSEGVREATRWDLTNAWPSHWRGAVLDAMSNEVAVESLSLVFETLQRG"
     gene            complement(10289..11527)
                     /locus_tag="VEV43_13020"
     CDS             complement(10289..11527)
                     /locus_tag="VEV43_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HYP24489.1"
                     /translation="MATTYLTPGIYVEEVPSGPRPIEAVGTRTAGFVGEAPAAKRRVN
                     EIVAINNWTEFLRNFSEESSASTPLSHAVYGFFQNEGSRCYVVNVGPGGNVAGGGRRR
                     EGLQLLETIDEIAIVAAPGYTDPASYDALLTHCETLKDRVAILDPPEEIESIVSLTQV
                     ATAASPRRASSGGDEEAVSTAAPAGGLRPRQSDAGFGAFYFPRITVRDPLPPGELVDV
                     APSGHIAGIWARSDARRGVHKVPANETVRGALNVSYNVTREEQGELNPAGVNVIRFFS
                     VEGTRVWGARTLADSASEWRYLNVRRLFNMIEESIEESTRWIVFEPNDETLWRSIRRD
                     VGAFLTRLWRDGALMGSTPQEAFFVQCDHETNPPDVIDAGMVITRIGIAPVKPAEFIV
                     FRISQFAGGAEVEIEGGARA"
     gene            complement(11529..>11791)
                     /locus_tag="VEV43_13025"
     CDS             complement(11529..>11791)
                     /locus_tag="VEV43_13025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYP24490.1"
                     /translation="SPMSVLHGVVLTQDDVPIPRARVDLPALDASTTTDSKGRFAFPR
                     VPSEPPTRLLHVSARGRQATYEADAVASPEDPLVLRFEIREG"
BASE COUNT         1676 a         4306 c         4165 g         1644 t
ORIGIN      
        1 tctcggcctc ccggatggtc aaaggatcag cttcacgatc gagacgatgg aggcgatcaa
       61 catcacgatc gcccagatga tggccaggat ggcgatgacg atcgccgcga tcccgtcggt
      121 gatcgagacc aggaacagcg ggacgatgcc gtggaggaac gcgacgatca cgctcgccag
      181 cagcgacgtc agcgtcagca gcacgatgct cttgttctgg aggaaccgct gctgcgccag
      241 caccaggcac acgcagaagc cgagcttcag cagcatgatc aggccgagga cgaccgacgc
      301 cccggttcgg tcgaagaacc cgtagatagc gaagtaggcg atggtcccga acggcacggc
      361 cgtgaggagc ccgaccatga tcatcagcag cacgaccgcg aagacgatcg cgatgatcag
      421 gccgatgacc agaaggatcg agaggatcag cccgacgatc ccctgcaccc gcccctggat
      481 gcgctccggc gccaccaggc tgaggcccat catcccgacc gcgtagagga gcaggccgtc
      541 caggaacgcc atgtactgga tggcgcgccc cggcgggtcc tcgtcccggc tcagctcctc
      601 gagctgggcg acgacgtcgc cgtccgtgac gtcttcgggc tcgagctcgt cgtcggggtc
      661 gcggttggcc tcctccaggg cgatgtcgcg cagctggccg gcggcgggcg cgaccccctt
      721 cacgagcgcg gcggaaccga tctcgaacag cacgacgatc gccatcagca cgatcgccag
      781 gacgaacagg ggacgcctca gctcatccat cgccgccgcc cgagaccggt tccgctcgct
      841 gtcgctcagg ccaggtagtc gcggacccgc gggggcgtcg cggcgaggtc ggtcgtccgc
      901 gtgccggggg tgccgccgtt cccgccgagg gccgaaccgc cgatcctgcg gatgatctcc
      961 agcgcgctga acccgtacgc gaccgcgtcg acgcgggtgt tcggctcgag caggaagccg
     1021 gcgacgaggc ccccgagcgc cacggtgatc acgaagacga cgtaccacca cggcttcttc
     1081 aagtacgtgg gcatctcggt cttgctctgg atcagcttga cgacgtcggg gagcgctccc
     1141 ccgatgcacc cgaacaagaa tgcggttccg tcactcaacg gcatcacact tctcctttct
     1201 tctcggcccg ctttgcgcaa ccacgatcac gacgcgacct ccagcacaca tgcctcgacg
     1261 gcggtacaga tgacggcgac ggtcgcctcg tcgcgcccga ggtcgatctc ggcacgcgcg
     1321 tcgtcaccag gagcgacgtc gacctcgacg tcgagcccct cgtcccgggc cagcgccaga
     1381 cgcgcggcgc cggcagtagc cacgagcctc agctcgcgcg agaacctcga ggactcggcg
     1441 acgccgaccg tgcagctgcc gcccgcgggg acgaggagaa cgctcccctg ccggcagccc
     1501 gcggagagct cgtccggctc caccgccggc acggggcgca ggaaccggtc ggcgagccac
     1561 gccgcgatgc cgtcccggcc ggcgtccccg tcgccgcggt cctgcccggc gccgatcccc
     1621 agcgcggcga cgaacaggag cagcacgacg acgaggacga cgatcacgac cttcatgcgc
     1681 gctcccgcgt cacagccagt actccacctc gtccggcgtg ccctcggcga tgaagcagcg
     1741 gcgcgtgaac gaccgagcga gctgccgggc gagctgcgcg tcctgctcgt tgtcgacgag
     1801 gacctggagt cccccaccgt ccgttacgac gaacccctgg gccccctggt cgaccacctg
     1861 cagggcgccg cggtcgtagg tcgtgcacgc gtcctcgggc ggcgtcgcga tccccgacgg
     1921 gtcgagccag tactcgacga tgtagtcgcg gcggttcggc ctggggttgc tccggccgat
     1981 gaagcaatgt tgcgagaacg acctcgccac gcggtaggcg ttgttcgcgt tcgtcgggtc
     2041 cgtcaggaag agcatgcgcg acccgccgtc ggtgatcagg tagccaccgg gctccgggac
     2101 gaggttgaga gcgttcgggt cgtaccccag gcagtctgcg cgcgggggct ccggcggcgc
     2161 cggtgtgggg gtctccgcgg gtggcggcga ctccgcggcc ggggacgcgg tcgtcgccgc
     2221 agccggcgtg ccgtcgtcac cccggctcag cagccagaac agcacgccgc cgatcacgag
     2281 gacgccggcg acgatcccga cgaggatccc tatcgggaac ttcttcgcag caggcgcggc
     2341 ccccacgtcg aacgcggacg acagccggtc cgagagctcg ctggggtcgg cctccgacac
     2401 gaccgcgaac gtgaacacgt acgagccggg cggcgcgccc tggccgttga tctggaccgt
     2461 cacctgcttc gtctcgttgg gggcccaatc gagcgtgctc tcgccgacga ccttgaacat
     2521 ctccggcttg gccgtgccct ccggcatgac cctgacgagg ccgcgcaccg cggtcgccgt
     2581 cacgttcgtg acggtgaacg cggcctcgcc gaccccgttg gtcagctcgg tcttggcggg
     2641 acccgttatc gcccaccact tagccatcgg tgacctcctc ctcttgtccg ggttgcatcg
     2701 aaagctcgta cgtcgcataa gcgggcttct gggcctcgag gatccggcgg accatcgcct
     2761 cgtagccgcg cgcggcggcc gggacggaca ccagcaggtg gaacgggtgc gcggtggaat
     2821 cggccgtgac gttctcgtag acggcgaagc cgcggacgcc cgtcgcgatc tcgaggaagc
     2881 gcgtgaggcc gtcgacggtc ccgcgcagct tcgacagcca cgccgccgag ccgatcagcg
     2941 cgcgcatcct gccggtcccc gacgctagcg gggcgaagtc gctcggcagc gcgtcctccg
     3001 ggttcgcgac catcagccag tcgaggtcca gccagcgcgc gagcatcggg acgccgtcgt
     3061 cgcgcgtgcg catcgggtcg aggtacgcgt cgagctcgac gagcacgcgc tcggccggct
     3121 cgtgcaggtg ctccatcacg tcgagcaccg gcccgagcgg gccgcccccg tcgatcgtgc
     3181 gttcgaagac ggagggcagg agcctccgga tctcagcttg ccgcaagctc gctcaccatc
     3241 tctagctcgt gctcgccgct ggagaacagc catgtcggcg ggagcgtcac gcgctcggtg
     3301 aactcagccg ccgagacgaa ctggtaggcg acgccgaagt cgtcgctgcg gcagagcccc
     3361 tcgggccccg cggtcagcag ccgcgtggcc gtggggtccg tcgcgagcga gcggaccggg
     3421 tggaaccgcc ccgggtcacg cagggggagg ccgcagcgca cgtcggcggc gatccactgc
     3481 gggttcaggc tcccggagtc gagccgcagc acccccgcgc tgtgcgtcgc ggcgaggacg
     3541 agcccgccgc ggaacgcgat cgaccagcac gtgccgccgc gccaccccac ctgacgcggc
     3601 acccagccct ccggcgggtt gtcctcaccg cggagctccc aggagtagca gcccttgccg
     3661 gggtcgtcgg agccgacgga cgcggtgccc gcccacagga acgcacgggg cccgtcctgt
     3721 tgcaccgcga gcacgcggac gtcctcgccg ttgaggccga tcgaacggaa gctgccgctc
     3781 cggcccccgt cgctcgacaa gaacgtgccg gccgcgtcct gcgcggcgag cgcgacgttg
     3841 acgcgccccg cctggtccgc ggcgacggcg acggagtaat agccgcggtc ggggttggcg
     3901 gcgtccacga cgacctgcaa cggcgtcgcg ccggcccgcg tcggggtgag ctcgtagagc
     3961 cccttgtcgg tagcgaggaa gagcagcggg gtctggtccc gcatcgacca cgcgacgtcc
     4021 tcgacgccga agccgagctg cacgccctcc tgccacgtct cgccgcagtc gacggagatc
     4081 tggaggtgcg acgcctcgtt gtcgaggcgg gagatcgccg cgaccagccc gggcacctgc
     4141 gggtgcgcac ggaccacctc gacgctcttg ccgggtagcc gcgccgccac ctcccagccc
     4201 tcgccgtcgt tgagcgagcg gaagaccgtc gtgcggcttc ccgcgtacca ggtcgacggc
     4261 tggaagtggt cgggcgcgac cgacagcacc tcttcgtcgg ggacctcgtc gacgagcagc
     4321 cggacccggt cgacgtagcg gacgccggct tcggcgagcg gcctgtcgta gacgttcgag
     4381 accctgagcg cctcgccgaa gcgccagccg cgcgtgccct gggtgggcag cgggttgatc
     4441 gtcgcgtgga gccgttcccg cacacgcctc tcgacggccg cgaggtcttc ctcccggtgc
     4501 acgacgacgc gggctcgcac gctcactgac ttgtagcgcg cccaccccac ctcgacgagc
     4561 gtcgccgcgg ggcgccggcg ctcgagcgcg gcggagacct cgagcaacgc gttgtccgtt
     4621 tggtgctcgg cgagcgttcc cgtcgtgagg gcgccgccga cgcgcaggtc gttcgggacg
     4681 tacgggacca tgaggagcga gaccgtcccc ggcagggcgt gccgccagag gttcgccttc
     4741 gtgaacgcgc gcgcccgcgc gaccgcgcct gagctgtgca gcgcgacgag ctggaagtcg
     4801 cgggcggtca ccgctcgctc gagcgaatgc agcgactgcg gcccccggat cagcgcgttg
     4861 tcgagggtct ccgcgggacg cccgccggtg gcaggcgacc ggttcgtcac ctcgacaccg
     4921 gggatcgggc tcttcatcgt cgtcagcgtc tcggcgccca ggttgccgtc cggccccccg
     4981 ccactgcggt accagacgcg gatctcgcgc ccggccttcg gcacgcccgc gagcgcgacc
     5041 ggcacgtctt cgagcgcacc gccctcgtcc gcgccccgca ccgcgggcgc gaacgagata
     5101 cgtccggcga cccggtcgac gtggaacacg tgcgggtcgt cgccggcgtt cgcgaagctg
     5161 ccgacctcgc tccagatccg gaacggcctc ccgtcgtgct ggatcgcggg cgcacgctcg
     5221 tcgagctcgt cggcggcggc ctcgaccccc acgagcaggt cgagggggtg ccccgtcggc
     5281 gccacgatcg gcggatgccc gacggccacg gtcaggcccg ggagccccgt ccccgtcgcc
     5341 acgagctcgg cctcggcgag cgtgcagtgg tgcgctaaga cgtcgacctc ggtcttgccc
     5401 ggcgggatcg tggctgcgtc ggccgtgacg aagacgggtg gcgttccgcc ggtcgtcgcg
     5461 cgcgccgcgg agacctcggt cccgcgcggg atctcgagcg cggtctcgct cgcctgcttg
     5521 cggctgaacg tgagcatcac gctcgccgcc ccggggggca tcagacggac gccgagcagg
     5581 ttgaggaacg cgacgtaagc cttgtccggc acgcggttca gccggtagat catcgtgtcc
     5641 gtcaggtacg cgaacagctc caggagcacc atcccggggt cgctcggggt caggtcgttc
     5701 cagtccgggg acagctggac gatccgcgtg cgcgcttgct cgagcaggtc ttcgaagtcc
     5761 ctgtcgtcga ggttcggcgt ggggagcgtc acgcgacctc ctctcccgag agctggacgg
     5821 ggagctccat ccggtcgagc ttcagcgtcg ccttcacgcg atagacgagc gtgagatcga
     5881 ggcgtccggg gtcggtcggg ttgcgccgtg cgtcgacgtc gagcaggtcg atgcgcggct
     5941 cccaccactc gagggcctgg cgcacgtagt ggatggcgag acccgcggtg gtgtcgtcgt
     6001 tcggcgagaa cacgacccgg tgcagcagac acccgtacgt cggccgcatc acgcgctcgc
     6061 ccgggatcgt cgacagcagc agcaggatcg cctggcggat cgagtcgtcc tcggcgacca
     6121 tctcgaaccc gacgcgcggc gacagccgga ggccgcccgc cgcgtccccc gccacgtccg
     6181 ggtggacgaa ccgcagtgcc cggaaccgcg cgcgctcgtt cacggcgcct ccgacacgaa
     6241 cgcctgcccc ggctgctcga cgacgtactt cacgaagccc gcgggggtcc cgtcggtgag
     6301 gccctggacc gagtcgaggc agacccggtc gccgtcgacg cgcagccact ccgagtagcc
     6361 ctgtttgacc gcgagcgtcg tcttgcacgg acggacgccg acgttgatgt tcgggcagcc
     6421 gttgatcgga cggccctcgg gatcggtcga cacgagcacg cggcggcctt ggatcgtcac
     6481 ccacggctgc gacggcgcgt tggagacgat cccggcctga tgcttgcaca ccagctttgc
     6541 gtcttcggtc agccagagca ctctctcgtc tcctcctagt ccggctcttc ggctcgcatg
     6601 aagtcgacgg tgttgccgcg cacgacgacg gccttcccgg gcgcgtcgat ctgcaggttg
     6661 gcctcggagt ggaggcgcac ggccgacggc gacatctcta ggaagctgcc ggtcgcgtcc
     6721 tccagccgca ccgccacggc ctcgtcgtcg aggacgacct tgtgaccccc ggcggtgagg
     6781 aacgtgaacc ggcgcacgtc gccgtccacg acgcccgcgt cgggcgggcc gtccgcgccg
     6841 tagaggccgc cgagcacgaa cgcgcgcgcg ggatcctcgt gcgcgagcac gacgagcacg
     6901 tggtcgtcga cgtcgggcag cgcgatcagc cccttgctcg gcccccctgc ggccatcacg
     6961 accgggatcc actccgtctc gacgtcgccg tgcgccggca gacggagcct gacccttccg
     7021 atcgagccgg ggtcgtcgac gtcggctacc accccgaccg tcacgctggc gccgcggggg
     7081 cgcaagcgcg cccggggcgg gtccgtcgag atctcggtga cgaaaccgtt gtcctggtcg
     7141 acggtgtgca cgaccgaagt gacgacgtag cggcccgaca ccgagtcggc gacgccggag
     7201 acgtcgacga cggtcgcggg ccgcaggtcc gcgtgcccgg gggccgcggc ccacagcgtg
     7261 acctccgccg cgacgcgcct gtcgagctcg gcctgagcca gcgcgtcggc ctgggcgtcc
     7321 gtgctcgtgc tctcgtccac gagcgaacgg tcgccgtccg caccgacgga agacgccggg
     7381 acgctcgcgg cgaccacgcg tcccgatccg gcctctgccg cggcgcccgt gcgcagctcg
     7441 accgtcgacg tgtcccagcc ggtcgcggcc acgccccctg ccgcggcgtc ggcgttcacc
     7501 gtgatccgca gctcgagcgc ggactcgccc agcacgaggc gaacgggctc gccgcggccg
     7561 cgcaggtcga ccagctcgag gcgtccgtcc agcacgactg gatagagccc gcaccggcgc
     7621 gtgacgtcga cgaggagctc gaggtcggac tgccggtact gcacgagcag cggccagacc
     7681 ggcccgtcgt gcgccgcgga gacgtcgaga cccagcggac ggacgagctc gctcgccagc
     7741 gcggcggcat cggagtcctt gaacgtgcgc accggctgcg tcttcttcag cctgtgcagc
     7801 acgtcgtagc cgcggacgcg cacgcgcttg ttgccgtcgg ccgcgtacac gcgctctatc
     7861 gcggtgacct cgccggagaa gagcgtcgcg tcgtcgaccg cgacctcgaa cggcgtcccc
     7921 ggctcgatgg cacggtcgtc gaccgacgca cgccagtggt cgtggaacgt cgcctcgcac
     7981 tgcgcgggga gagcgagctc gtgcctcacg ctgagcccga cgagcgtctc gccgagctcg
     8041 agcggcagct ctcctccacc gagcgagacg gcgaccctgg gaagggtgag caccctcgtc
     8101 acgacgcgct cgcaaagacc gagaggggcg ggatgcgcag caacgtcccc ggcgggacgt
     8161 ggagcgggtc gccgatgccg ttgaacgtcg cgatcacgcg ccacagcgac gcgttgccgt
     8221 agtagcgctc ggcgatcgcg tcgagtcgtt cgccgggcgc gtcgggccct gcaccagcga
     8281 cctcgtgcac catgacgttg tccggcggga cctcgccccc gcgcgggagc acgagggtgc
     8341 cgggtcccgg cgcggccgcc gtgggaggcg gcggctcggc cgagcgcagc agccgcatcc
     8401 ggagccacga ccgccgcggg gctcccgacg ccgcgaactg ctcgacgcgc tccgcgaccg
     8461 cctggacgat ccccgggtag ttccacacct tgccccacag gaacctgacg agaggcgggc
     8521 gcccgtaagc gccctcggag cgcgcgttct ccgcgagctg ccacaacggg cgcgtgaggt
     8581 cgcggacgtc ctcggacgcg ccctcgcccg cgaggaacgt gtcgaacagc aggtccagct
     8641 ggagctcggt cgcccctccg cccgtgtaca gcaacgggtc gtccatcagc cgcgtgcctg
     8701 tgagctggcc cgagacgccc cggcgcgacc ggaggccggc gcgccgcttc atgacgagcg
     8761 actcggggtt cagcagcgcc gacaaccgtt cgcccgtctc ctcgacgagg aatgcgacgc
     8821 gctccatcag aggctcctca gctctgagtc gagcgatgcg cgacggtcgc gttccagcag
     8881 cgcctgctgc ggcacgtcct cgtcgtcgga gatgcccggt gggagctcgg gccagctgcc
     8941 ttcgtcgtcg tgcagcgtcg cggcgggtgc gacggaggga tcgtccggca cgtcgggcca
     9001 ccccgctgcg atgccgatct ccgccgtaac ggccgtctct tccggctcga acgcctgcac
     9061 ccaccggggt tcgagagacg cggggacgct cgaaggcgag gccttgccgg acggatgcat
     9121 cgcgaacgcg tcggccagcc gtgggaacgc cgcggccctc cgctcacgag tcgtctcgat
     9181 cgccgagcgc cggggtcgcg tcgcgaaccc gactcggccg ggcggtgccg ccttcttctc
     9241 cgggggctcg ggcgccggct cgtccgggac cacgtccgcg tccggccggg gcggcggcgc
     9301 gtccggcgcg ggaggctcgg cgtcgcgccg gcgcccgtcg cgcgcgggcg tgcgctcgac
     9361 ctctgcccgc acggccgcga cgaccttcgt ggtgagcgtg atgccgtccg ccacgcgcgc
     9421 gcgggcccgg gtgacgatcg ccggcaccgg cggacgcgga cgtgcgggag catccggctc
     9481 gatcgcggcc tcttgcaccg ccggccgcgg cgccgcagcg agcgagctcc gaagccgtcc
     9541 gggtcgcgcg ggttcgcccg cagtcgccgc cgagggtcta ccggccgggc cgaacggagg
     9601 cggcagctcg tgacgaagga cgtcctcggc ctcgggctcg agcaaccacg gcgccttggc
     9661 ccgtacccgc gccgcccagt cggccggcgg gcctcccgcc ggatgttcgg ggccgcccca
     9721 acggcgcgcc catccgtcga ggacccccgc gaccgcgcgg gcgaccgccg cgagcccggc
     9781 tacgacacgg ccggtgagct tccgcagcat cggtcgctat ccccgctgca gggtctcgaa
     9841 caccagcgac agcgactcga ccgcgacctc gttcgacatc gcgtcgagca ccgcgccgcg
     9901 ccagtgcgac ggccatgcgt tcgtgaggtc ccagcgcgtc gcctcgcgca cgccttcgga
     9961 gtcgagcagc aggatcgaga cgttccgacg ttcgaccttg ccctggactc cggtcatgaa
    10021 ccactgccac aggtctcggg actgcgtcag gccgtagcgg agctcgaccg tgccgtagtc
    10081 gacgcggccc gggatcgcgt gcacgacctg ctgcgtcccg ccctcgcggt agcggatcgg
    10141 ctggatcttg acctcgaggc cgcggcactc gacgaagtgg ccttcgacga cgccgccgat
    10201 gctcagcttg aagttgtagg agcggtaggg gtcgacgaac gtaccgggct gtgcgccgga
    10261 tgccgccggg gctgctgtgg gggccgcttc aggcacgtgc tcctccttcg atctcgactt
    10321 cggcgccgcc tgcgaactga ctgatccgga agacgatgaa ctcggccggc ttcaccggcg
    10381 cgatcccgat gcgcgtgatg accatgcctg cgtcgatcac gtcgggcggg ttggtctcgt
    10441 ggtcgcactg gacgaagaac gcctcttgcg gggtcgagcc catcaacgcg ccgtcgcgcc
    10501 acagcctcgt gaggaaggcg ccgacgtcgc ggcggatcga gcgccacagc gtctcgtcgt
    10561 tcggctcgaa gacgatccac cgggtgctct cctcgatcga ctcctcgatc atgttgaaga
    10621 gccggcgcac gttcaggtag cgccactcgc tggcgctgtc ggcgagcgtg cgcgctcccc
    10681 agacccgcgt gccctcgacg gagaagaacc ggatgacgtt gacgcccgcc gggttgagct
    10741 ctccctgctc ctcgcgggtg acgttgtagc tgacgttgag cgcgcccctc acggtctcgt
    10801 tcgccgggac cttgtgcacg ccacggcgcg cgtcgctgcg cgcccagatg ccggcgatgt
    10861 gacccgacgg cgcgacgtcg acgagctcgc ccggaggcag cgggtcccgc accgtgatcc
    10921 tcgggaagta gaaggccccg aagcccgcgt cggactggcg ggggcgcagg ccgccggcgg
    10981 gggccgcggt gctcacggcc tcctcgtcgc cccccgacga cgcccgccgc ggcgaggccg
    11041 cggtcgcgac ctgcgtcagc gagacgatcg actcgatctc ctccggcggg tcgaggatcg
    11101 ccacgcggtc cttgagcgtc tcgcagtgcg tcagcagcgc gtcgtacgag gcggggtcgg
    11161 tgtacccggg ggccgcgacg atcgcgatct cgtcgatggt ctcgagcagc tgcaggccct
    11221 cgcggcgacg cccgccgccg gcgacgttcc cgccggggcc gacgttcacg acgtagcagc
    11281 ggctcccctc gttctggaag aagccgtaca ccgcgtgcga gagcggtgtg ctcgccgagc
    11341 tctcctccga gaagttgcgg aggaactcgg tccagttgtt gatcgcgacg atctcgttga
    11401 cgcgtcgctt tgccgcgggg gcctctccga cgaacccggc cgtcctggtg ccgacggcct
    11461 cgatgggccg cggccccgag gggacctcct cgacgtagat ccccggtgtc aggtacgtcg
    11521 tcgccacgtc atccctccct gatctcgaaa cgcaacacca acgggtcttc cggtgaagcc
    11581 actgcgtccg cctcgtacgt cgcctgccgg ccgcgggccg agacgtgcaa cagtcgtgtg
    11641 gggggctccg acgggacgcg cgggaacgcg aagcgcccct tcgagtccgt ggtcgtcgag
    11701 gcgtccagcg cggggaggtc tactcgcgcc cgcgggatcg gcacgtcgtc ctgcgtgagc
    11761 acgacaccgt gcagcaccga catggggctg g
//
LOCUS       DASQDO010000474         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_418001, whole genome shotgun sequence.
ACCESSION   DASQDO010000474 DASQDO010000000
VERSION     DASQDO010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418001"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VEV43_13030"
     CDS             <1..141
                     /locus_tag="VEV43_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP24491.1"
                     /translation="ADSGQALASLDALAATGAVVVLTGHGEPWTQGVASAVERAREAG
                     PS"
     gene            complement(138..629)
                     /locus_tag="VEV43_13035"
     CDS             complement(138..629)
                     /locus_tag="VEV43_13035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYP24492.1"
                     /translation="MSKLADRGGTVTTKATLHTNHGAIEIELFDADAPKTVENFTKLA
                     RDGFYDGTIFHRVIPDFMIQGGDPTGTGTGGPGYQFDDEQNEHSVERGMLAMANAGPN
                     TNGSQFFIVTTESAPWLNGKHTVFGRVTSGMNVADAISQVERDARDKPLEDVVIERVE
                     LAP"
     gene            complement(744..1100)
                     /locus_tag="VEV43_13040"
                     /pseudo
     CDS             complement(744..1100)
                     /locus_tag="VEV43_13040"
                     /inference="COORDINATES: protein motif:HMM:NF025390.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
     gene            complement(1187..>1712)
                     /locus_tag="VEV43_13045"
     CDS             complement(1187..>1712)
                     /locus_tag="VEV43_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HYP24493.1"
                     /translation="FIARATVETLKDYPWINGEIRGDQIVTRSHVNLGFAVELADGKG
                     LIVPVLRNCETLNLLGLAKGVADLARRAREKQLLPDDVVGGTFTITNPGGYGTFHGTP
                     VISQPQSGILGTYAVVKRPWVVQDDLGEDVIAIRSIMNLTLTYDHRLVDGALAGRFLR
                     DLRGRLESWGESDY"
BASE COUNT          238 a          597 c          590 g          287 t
ORIGIN      
        1 gcggacagcg gccaggcgct ggcctcgctc gacgcgctcg ccgcgaccgg cgccgtggtc
       61 gtgctcacgg gccacggcga gccctggacg cagggagtcg cgtctgcggt cgaacgtgcg
      121 cgcgaagccg ggccgagcta gggcgcgagc tcgacgcgct cgatcacgac gtcctccagc
      181 ggcttgtcac gggcgtcgcg ctcgacctgc gagatcgcgt ccgcgacatt catcccgctc
      241 gtcacccggc cgaagaccgt gtgcttgccg ttcagccagg gtgccgactc cgtcgtgacg
      301 atgaagaact ggctcccgtt ggtgttcggc ccggcgttcg ccatcgccag catcccgcgc
      361 tcgacgctgt gctcgttctg ctcgtcgtcg aactggtagc cgggcccacc cgtcccggtt
      421 ccggtcgggt cgccgccctg gatcatgaag tccgggatca cacggtggaa gatcgtcccg
      481 tcgtagaacc cgtctcgcgc gagcttcgtg aagttctcga ccgtcttggg agcgtcggcg
      541 tcgaagagct cgatctcgat cgcgccgtgg ttcgtatgca gcgtggcctt ggttgtcacg
      601 gttccacccc gatcagcgag tttgctcagg cgcgggacga tcgcagacag ctccttctcc
      661 gtccactcct cgccggacgg ctccttgggg tcggacggtc cggagacggg aagcggcttc
      721 cccgtcgcgt cttcgtacgc ctcctcgcgg acgtaggaaa cgacctccca gtcgagctcc
      781 gcaggcagct tcacgtccgc gagcgagtcc gggtctcgca gcgccgcctc gtaggtctca
      841 cgacccgctg cgacgaggta gtcgcggaag tacgagaagc agtcgtccga gcagccgccg
      901 ttgatcacgt atgccgccgc ccacagatcc cagcggtacg cctcgaccgt tcgcgcgcgc
      961 cagagccggt cgaacgtgac gatgtccgcc ggcggcagct tcgcgagctc gccccggaga
     1021 gcgtcggctc gcgcgcccat gtcatcggcc ggcgaggacc tcgcgtcgtc gatcaggctc
     1081 cagaatcgtt cctcgttcat cgccgcggcc gacggcggcg gggaggcctg ctccggtgtc
     1141 tccgtcgagc cgcaccccac gaggccgacg gcggcggcga gcgcggctag tagtccgact
     1201 cgccccacga ctcgaggcgc ccgcgcaggt cgcgcaggaa gcgtccggcc agggcgccgt
     1261 cgacgaggcg gtggtcgtac gtgagcgtca ggttcatgat cgagcggatc gcgatcacgt
     1321 cctcgcccag gtcgtcctgg acgacccacg ggcgcttcac gacggcgtac gtgccgagga
     1381 tcccgctctg cggctggctg atcacgggcg tcccgtggaa ggtgccgtag ccgccggggt
     1441 tcgtgatggt gaacgtgccc ccgacgacgt cgtccggaag gagctgcttc tcgcgggcgc
     1501 gccgcgcgag gtctgcgacg cccttcgcga gtccgagcag gttgagcgtc tcgcaatttc
     1561 gaagcacggg gacgatcaga ccctttccgt cggcgagctc gacggcgaac ccgaggttca
     1621 cgtgcgagcg cgtgacgatc tggtcgccgc ggatctcgcc gttgatccac gggtagtcct
     1681 tgagggtctc gaccgtggcg cgcgcgatga ac
//
LOCUS       DASQDO010000475         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_478512, whole genome shotgun sequence.
ACCESSION   DASQDO010000475 DASQDO010000000
VERSION     DASQDO010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478512"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..623
                     /locus_tag="VEV43_13050"
     CDS             45..623
                     /locus_tag="VEV43_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase accessory protein
                     MauD"
                     /protein_id="HYP24494.1"
                     /translation="MEGWWAASYVLLWILVVVLVVVVVALARQIGTLHLRLGPRGALE
                     LDDEGPPLGEAPPHEETTDAHGQSARIGGPGEGQLLLFVSPGCHLCEQVLPGLAVVAR
                     KHELAPYVLADVDPDETVSQYERRRLNVRVVSGHLIAQRYRVPGTPYVVVLDRLGVVR
                     AKGTVNNLEQLEGLVETGARRSEEAEQERRAS"
     gene            620..1174
                     /locus_tag="VEV43_13055"
     CDS             620..1174
                     /locus_tag="VEV43_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24495.1"
                     /translation="MIPKQRLGAVPRAAENATIAIAERTSRRSFIGRVGRGFVALVGG
                     SVVASALAPERAEAYHVCGHTYTTGSCPHPFTPESRVDRHGYPIHPKYGYPVDDDGNV
                     YTSREQTRRKMCQEVVPARYPHTTGADYGGGWSRCCNGRIRRIADCCSYSQTRINGDA
                     SVTGYCYNGTKVFCIGYRDTNVRC"
     gene            1263..1826
                     /locus_tag="VEV43_13060"
     CDS             1263..1826
                     /locus_tag="VEV43_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24496.1"
                     /translation="MAETITPVVHGGRRGPYLVASALHVAGATASAAVLGIVLGGLGA
                     LARAPWDATPFVVAALAVVYCAREAFALPIPLPDRKRQVPEWWRTFYSPAVAALLYGA
                     GLGIGFLTYLSFGTFAVVMAGAVVSGDPLQGALLCGAFGAARGLAVVAVTWNGPRDLD
                     EVAASPGPRLANAAVLACVAGVAALAA"
     gene            1914..>2813
                     /gene="groEL"
                     /locus_tag="VEV43_13065"
     CDS             1914..>2813
                     /gene="groEL"
                     /locus_tag="VEV43_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYP24497.1"
                     /translation="MPKVLSFNEEARRALERGMNQLADAVKVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAMEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQAMVREG
                     LRNVAAGANPIALKRGIEKAVESAVEAIRGSAREVEGRDQIAQVASISANNDYEIGET
                     IAEARDKVGKDGVITVEESNTFGLELELVEGMRFDKGYISPYFVTDAERMEAVMDDPY
                     ILIANQKISAVKDLLPVLEKVMQSGRPLAIIAEDVEGEALATLVVNKIRGLFKSVAIK
                     APGFGERRKAMLQDIAILTGGQVV"
BASE COUNT          435 a          970 c          984 g          424 t
ORIGIN      
        1 gggtgtcgtc gtgtttgccg gccccggcgg tagcctcgga gtagatggaa ggttggtggg
       61 ccgcctctta cgttttgttg tggatcctcg tcgtcgtgct ggtcgtcgtc gtcgtggcgc
      121 tcgcccggca gatcgggacg ctccacctcc ggctcggccc ccgcggcgcg ctcgagctcg
      181 acgacgaagg gccgcccctg ggcgaggctc cgccgcacga ggaaaccacc gacgcgcacg
      241 gccaatccgc gcggataggg gggccgggtg aaggacagct cctgctgttc gtgtcgccgg
      301 gatgtcactt gtgcgagcag gtgctcccgg gcctggcggt cgtggcgcgc aagcacgagc
      361 tcgcccctta cgttctcgcc gacgtcgacc ccgacgagac cgtgtcgcag tacgaacgcc
      421 gccggctcaa cgttcgcgtc gtgtcggggc acttgatcgc tcagcgttac cgcgtgcccg
      481 ggacgcctta cgtcgtcgtg ctcgaccggc tcggcgtcgt gcgcgcgaag gggaccgtga
      541 acaacctcga gcagctcgaa ggactcgtcg agacgggggc ccgtcgctcc gaggaagcgg
      601 aacaggaaag gcgcgcgagt tgataccgaa gcagcggctc ggcgctgttc cacgagcagc
      661 cgagaacgca acgatcgcca tcgccgaacg gaccagccgg cgatcgttca tcgggagggt
      721 cggccgcggc ttcgtcgcgc tcgtcggcgg gtccgtcgtc gcgtcggccc tcgcacccga
      781 gcgcgccgag gcgtaccacg tgtgcggaca cacctacacg accggctcct gcccgcatcc
      841 gttcacgccc gagtcgcgcg tcgaccgtca cggctatccc atccacccga agtacggcta
      901 tcccgtcgac gacgacggga acgtgtacac gtcgcgcgag cagacgcgcc gcaagatgtg
      961 ccaggaggtc gtgccggcgc gctacccgca cacgaccggc gcggactacg gcggcggctg
     1021 gagccgttgc tgcaacggga ggatccgcag gatcgcggac tgctgctcgt actcgcagac
     1081 ccgcatcaac ggcgacgcga gcgtgaccgg ctactgctac aacggcacga aggtcttctg
     1141 catcggctac cgcgacacga acgtccgctg ctagcgagtt ggccgagccc ctcaccgcgg
     1201 tcctcgtgat cgcgtcggtc gtagccggga tctcgacgct cttctctccc tgaggcctct
     1261 cgatggcgga gaccatcacc cccgtggtcc acgggggaag gcgggggccg tacctcgtgg
     1321 cgagcgcgct gcacgtcgcc ggcgcgaccg cgtcggccgc cgtgctcggg atcgtcctcg
     1381 gagggctcgg cgcgctagcg cgggcgccgt gggatgcgac gcccttcgtc gtggccgcgc
     1441 tcgccgtcgt ctactgcgcg cgggaggcgt tcgcgctccc gataccgcta ccggatcgca
     1501 agcgacaggt tcccgagtgg tggcggacgt tctactcccc cgccgtcgcc gcgctcctgt
     1561 acggggccgg gctcggcatc ggcttcctca cgtacctgtc gttcggcacg ttcgcggtcg
     1621 tgatggcggg agcggtcgtg tccggcgatc ccctccaggg cgcgctgctg tgcggtgcgt
     1681 tcggcgcggc gagagggctg gccgtcgtcg cggtgacgtg gaacggcccc cgggacctgg
     1741 acgaggtcgc cgcgtcgccc ggcccccgcc tcgccaacgc cgcggtgctg gcgtgcgtcg
     1801 ccggggtcgc cgcgctcgcg gcctgagctc atccccgact tgcggacctg ccgtgctctg
     1861 gctatcttta gcactcagag gatcagagtg ctaatccacc caggaggtag cagatgccga
     1921 aagtcctgtc tttcaacgag gaggccaggc gcgcgctcga gcggggcatg aaccagctcg
     1981 ccgacgccgt caaggtcacg ctcggcccca agggccgcaa cgtcgtcctc gagaagaagt
     2041 ggggcgcgcc cacgatcacg aacgacggcg tgtcgatcgc catggagatc gagctcgagg
     2101 acccgtacga gaagatcggc gccgagctcg tcaaagaggt cgccaagaag accgacgacg
     2161 tcgccggtga cggcacgacg acggcgaccg tcctcgcgca ggccatggtc cgcgagggcc
     2221 tccgcaacgt agccgccggc gccaacccga tcgcgctgaa gcgcgggatc gagaaggccg
     2281 tcgagtccgc cgtcgaggcg atccgcggct cggcgcgcga ggtcgagggc cgcgaccaga
     2341 tcgcccaggt cgcctcgatc tccgcgaaca acgactacga gatcggcgag acgatcgccg
     2401 aggcgaggga caaggtcggc aaggacggcg tgatcacggt cgaggagagc aacacgttcg
     2461 gcctcgagct cgagctcgtc gagggcatgc gcttcgacaa gggctacatc tcgccgtact
     2521 tcgtcaccga cgcggagcgg atggaagcgg tcatggacga cccctacatc ctgatcgcga
     2581 accagaagat ctccgccgtg aaggacctgc tgcccgtgct cgagaaggtc atgcagtcgg
     2641 gtcgcccgct ggcgatcatc gccgaggacg tcgagggcga ggccctggcc accctggtcg
     2701 tcaacaagat ccgtggcctg ttcaagtcgg tggccatcaa ggcccccggc ttcggcgagc
     2761 gccgcaaggc gatgctgcag gacatcgcca tcctcaccgg cggccaggtg gtc
//
LOCUS       DASQDO010000476         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_601015, whole genome shotgun sequence.
ACCESSION   DASQDO010000476 DASQDO010000000
VERSION     DASQDO010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601015"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VEV43_13070"
     CDS             <1..195
                     /locus_tag="VEV43_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24498.1"
                     /translation="EGPGLEYFELHGELDVEHAGELAAAIAELSSSEEDLRDAEEGAA
                     AGARAIRGLLDGVARVRGIG"
     gene            192..932
                     /locus_tag="VEV43_13075"
     CDS             192..932
                     /locus_tag="VEV43_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYP24499.1"
                     /translation="MSLTHARRVAALYDVHANPFALDAVLADASSRDVDLLVFGGDLA
                     WGAFPRQALERVVDPGLPALYVRGNADREVAAPAGDGWEVEIARWCSQQLDDDQRRFL
                     HTQAETVTVEVEGIGEVLFCHGSPRSDEESLTFLTPDERMREAVGAVSAKVVVCGHTH
                     MQFDRTCGDVRVVNAGSVGMPYEGRPGAYWCMLDASGISLHRTEFDFAAASEAVASSG
                     CPHAEEMAKDVMSPPGRQETAELFEPRD"
     gene            complement(933..1982)
                     /locus_tag="VEV43_13080"
     CDS             complement(933..1982)
                     /locus_tag="VEV43_13080"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HYP24500.1"
                     /translation="MTRNAKRAAIIGGAVLLLAGGIFFVLVNRDAIGDVPIVGDVLGE
                     DPKCPLTSRKPVSEARVDRPAVAIKIENNPVAYPLSGLENAEIVYEEPVEGGLTRFMA
                     IYHCTDAPQAGPVRSARIVDPPIMTPYTRILGAAGGNDIVMNALNEADVITIDETRAR
                     DAMERIDRPGITSEHTLYADTAAIRKLGRKQFDEAPPDDLFEFGDLQGTGRRARSVTL
                     TFSAGAVVTYEWEGERWSRSDGDAPLANDAGEQIQVDNVIIEEHTVNHSAELGDVLGT
                     PSPEIEDVTGSGRAFLFRDGRVFAGKWTRESTDEPVRFETNKGDAFVLHPGTTWIELL
                     PDKQGDVKGSFTISK"
     gene            2172..>3592
                     /locus_tag="VEV43_13085"
     CDS             2172..>3592
                     /locus_tag="VEV43_13085"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HYP24501.1"
                     /translation="MLWFKGAVSKRIVFVALAALLATTAGLPGRGTQAESRERYRVRK
                     ERIAPGLVLLRINDRRGPNRIRVLRVDATRELSLDTVLANDTRPGHETTRSRAERSGA
                     IAAINGDYTLLPSDPGAGRPVNLFSTDGELITSSLIWGRNFALSHDEQSAYVGHPSVS
                     ITALQHDSGELWRIRDWNENQIEPEGYSVYTPEGGSLFRPPGNSCSVRLAAPGAMEWN
                     EDGSGIQQELLVEAFRCSTRPMARRDGIVISAPQGTRHARSMEASLVVGEGMTVTWSA
                     GWPGVLDTIGGNPTLLEDGVITAEACDDSYFCDRNPRTGVGVTARGKILLVTVDGRQE
                     KSVGMTPMEFAKLMQHLGATSALNLDGGGSTAMWVEGGLVNRPSDVPERAVGSALVVL
                     PGPDIAEPVPAPYTPPSPVPSPVPSPSVSPSPSISPSAGFDVAPVPLVPRSGFERALP
                     FPPSPRCAGLHDAGSTGGMLDALA"
BASE COUNT          485 a         1247 c         1298 g          562 t
ORIGIN      
        1 gaggggccgg gtctcgagta cttcgagctg cacggcgagc tcgacgtcga gcacgcgggc
       61 gagctggcgg ctgccatcgc cgagctgagc tcgtcggagg aggacctgcg cgacgccgaa
      121 gagggcgccg ccgcgggggc acgggcgatc cgcgggctgc tcgacggcgt cgcacgcgtc
      181 cgcgggatcg gctgagcctc acgcacgccc ggcgcgtagc cgccctctac gacgtccacg
      241 cgaacccctt cgcgctggac gcggtgctgg ccgacgcgtc gtcccgcgac gtcgaccttc
      301 tggtcttcgg cggcgacctg gcctgggggg cgttcccgag acaggcgctc gaacgcgtcg
      361 tcgaccccgg gctgcccgcg ctctacgtcc gcgggaacgc ggaccgcgag gtcgccgcgc
      421 cggccggcga cggttgggag gtcgagatag cgcgctggtg ctcccagcag ctcgacgacg
      481 atcagcgccg gttcctgcac acgcaagccg agacggtgac ggtggaggtc gaagggatcg
      541 gcgaggtgct gttctgtcac ggctcgccgc gcagcgacga agagagcttg acgttcctca
      601 cgcccgacga gcggatgcgg gaggcggtgg gagccgtctc ggcgaaggtc gtcgtgtgcg
      661 gccacacgca catgcagttc gaccgtacct gcggcgacgt ccgggtcgtg aacgcgggca
      721 gcgtagggat gccgtacgag gggcggcccg gcgcgtactg gtgcatgctc gacgcgtcgg
      781 ggatctcgct ccaccgcacc gagttcgact tcgccgcggc gtccgaggcc gttgcctcct
      841 ccgggtgccc ccatgcagaa gagatggcga aggacgtgat gtcgccgccg gggcgccagg
      901 agaccgcgga gctgttcgaa ccccgcgatt agctacttcg agatcgtgaa cgagcccttc
      961 acgtcgccct gcttgtcggg caggagctcg atccacgtcg tcccgggatg cagcacgaac
     1021 gcgtcgccct tgttcgtctc gaagcggacc ggctcgtccg tgctctcgcg ggtccacttg
     1081 ccggcgaaga ccctgccgtc gcggaacagg aacgcgcggc ccgacccggt gacgtcttcg
     1141 atctcgggag acggcgtgcc gaggacgtcg ccaagctccg cggagtggtt gaccgtgtgc
     1201 tcctcgatga tcacgttgtc gacctggatc tgctcgccgg cgtcgttcgc cagcggggcg
     1261 tcaccgtcgc tgcgcgacca ccgctcgcct tcccattcgt aggtcacgac cgcgccggcg
     1321 ctgaacgtca gcgtcacgga gcgcgcacgg cgcccagtcc cctggaggtc gccgaactcg
     1381 aacaggtcgt cgggcggggc ctcgtcgaac tgcttgcggc cgagcttgcg gatggccgcg
     1441 gtgtcagcgt agagcgtgtg ctcagacgtg atgcccgggc ggtcgatgcg ctccatcgcg
     1501 tcgcgtgccc tcgtctcgtc gatcgtgatg acgtcggctt cgttcagcgc gttcatcacg
     1561 atgtcgttgc cgccggccgc gccgaggatg cgcgtgtacg gcgtcatgat cggggggtcg
     1621 acgatgcgcg cgctgcggac cggcccggcc tgcggcgcgt cggtgcagtg gtagatcgcc
     1681 atgaagcggg tcaggccccc ctcgaccggc tcctcgtaga cgatctcggc gttctcgagg
     1741 cccgacagcg gataggccac cgggttgttc tcgatcttga tcgcgacggc cgggcggtcg
     1801 acgcgcgcct cgctcacggg cttgcgcgac gtaagcgggc acttcgggtc ttcgccgagg
     1861 acgtcgccca cgatggggac gtcaccgata gcgtcgcggt tgacgaggac gaagaagatg
     1921 ccgcctgcga gcaatagaac ggcgccgccg atgatcgccg cgcgcttcgc gttcctcgtc
     1981 aacgtccctc cttagggttc gggtcggggg cccctcgggg aggtgtcgtc tgggcgattt
     2041 ccccgggccg gacgggagca cggcgaccgc cttcccatcg gcggacgacg gtatcacggc
     2101 gcgctcggca acctctggat tcggtcgcct cgcttgcagc gttcccgcag ggtcttgcta
     2161 ctctgctgac ggtgctgtgg ttcaaggggg cagtgagcaa acggatcgtc ttcgtcgcgc
     2221 tcgccgcgtt gctcgcgacg acggccgggt tgcccgggcg aggcactcag gcggagtcgc
     2281 gcgagcgcta ccgcgtccgc aaggaacgca tcgccccggg gctcgtgctg ctgcgcatca
     2341 acgaccgcag ggggcctaac cgcatccgcg tcctgcgcgt cgacgcgacg cgcgagctct
     2401 cgctcgacac cgtcctcgcg aacgacacgc gcccgggtca cgagacgacg cgctcgaggg
     2461 ccgagcgcag cggcgcgatc gccgcgatca acggcgacta cacgttgctc ccgtccgatc
     2521 ccggcgcggg gcggcccgtg aacctgttct cgaccgacgg cgagctgatc acctcgtccc
     2581 tgatctgggg ccgcaacttc gcgctgtcac acgacgagca gagcgcctac gtcgggcacc
     2641 ccagcgtctc gatcacggcg ttgcagcacg actccggtga gctctggagg atccgcgact
     2701 ggaacgagaa ccagatcgag cccgagggct actccgtcta cacccccgaa ggcgggtcgc
     2761 tgttccggcc ccccgggaac tcgtgctcgg tcaggcttgc ggccccgggg gcgatggagt
     2821 ggaacgagga cggctccggg attcagcagg agctgctcgt cgaggccttc cgctgctcca
     2881 cgcgtccgat ggcgcggcgg gacggcatcg tgatctccgc gccgcagggc acgcggcacg
     2941 cccgctccat ggaggcgtca ctcgtcgtcg gcgagggcat gaccgtgacg tggtcggcgg
     3001 ggtggccggg ggtgctcgac acgatcggcg ggaacccgac gctgctcgag gacggcgtga
     3061 tcacggcaga ggcttgcgac gactcgtact tctgcgaccg caatccgcgt acgggggtcg
     3121 gcgtgacggc ccgcgggaag atccttctcg tcacggtcga cggacgtcag gagaagtccg
     3181 tcggcatgac gccgatggag ttcgcgaagc tgatgcagca cctcggcgcg acgtcggcgc
     3241 tcaacctcga cggcggtggc tcgactgcga tgtgggtcga gggcggcctc gtcaaccgcc
     3301 cgtccgacgt gcccgagcgc gcagtgggct cggcgctcgt cgtgctgccg gggccggaca
     3361 tcgcggagcc cgtccccgcg ccctacacgc ccccgagccc cgtgccgtcg ccggtcccgt
     3421 ctccgtcggt gtcgccgtcg ccgtcgatct cgccgagcgc ggggttcgac gtcgcgcccg
     3481 tgccgctcgt ccctcgcagc ggcttcgagc gcgctctccc cttcccgccg tcgccgcgct
     3541 gcgcgggcct gcacgacgcc gggtcgaccg gggggatgct cgacgcgctc gc
//
LOCUS       DASQDO010000477         4157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_723535, whole genome shotgun sequence.
ACCESSION   DASQDO010000477 DASQDO010000000
VERSION     DASQDO010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723535"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VEV43_13090"
     CDS             complement(<1..402)
                     /locus_tag="VEV43_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYP24502.1"
                     /translation="MRPKSLAALVALLVAGLGTPSRAAPSYVAETVVYKIETRAGTLN
                     AEVVHATLNGKIVKAPAIMTMSPYSALGRNGDADRWVAKGYHRVWVDVPGTGDSGGCY
                     DYGADGEKKAGYDSVEWVADQKWSTGKVAMIG"
     gene            459..1892
                     /gene="gltX"
                     /locus_tag="VEV43_13095"
     CDS             459..1892
                     /gene="gltX"
                     /locus_tag="VEV43_13095"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HYP24503.1"
                     /translation="MATVKTRLEPAPSGSIHVGNARTGLFCWLFAKHHGGSFVLRIAD
                     TDAKRVTEENYQAVLEDLRWLGLEWDEGPEIGGPNGPYRQSERFHLYTEKANELLEKG
                     FAYPCYCTPEELELERKTAHEESRAPGYSGRCRNLTDDERRAFEAEGRKPSLRFAVPA
                     DRTITVEDAVLGELSTDLGRTPDFVIQRSDGSPTYMLAVTVDDVAMGITHVIRGNDLV
                     ASTARQLLLREAFGVEEMPVFAHLPLLVSTEGKPLSKRWGDVSVAAYREQGFLPEAMV
                     NYLALLGWSYDDKTNVFSVDELVERFSLERVGKNPAAFDVAKLEWLNGHYVRALTPAE
                     LADRLVPFCERAGLAADSPEGREILTGVAPLISERLKRLDEAPPMIRFLFEDVEPEGK
                     AAKALDGQDDYLAAVAGGLAALDKWTTPAIEEVLRGLAEERGLKPKQAFQPVRAAVTG
                     TLVSPPLFESIELLGRERTLRRLGYPA"
     gene            1910..1982
                     /locus_tag="VEV43_13100"
     tRNA            1910..1982
                     /locus_tag="VEV43_13100"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1944..1946,aa:Gln,seq:ctg)
     gene            2005..2079
                     /locus_tag="VEV43_13105"
     tRNA            2005..2079
                     /locus_tag="VEV43_13105"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2040..2042,aa:Glu,seq:ctc)
     gene            2116..2463
                     /gene="rplS"
                     /locus_tag="VEV43_13110"
     CDS             2116..2463
                     /gene="rplS"
                     /locus_tag="VEV43_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYP24504.1"
                     /translation="MNRTDLVERPYLRDDLPTFRAGDTVKVHVRVVEGERERIQVFEG
                     IVIRRRGSQLSETFTVRKISFGVGVERTFPVHSPMIAKIEVGARGDVRRAKLYYLRSR
                     VGKKAKVKELRDR"
     gene            2473..3168
                     /gene="lepB"
                     /locus_tag="VEV43_13115"
     CDS             2473..3168
                     /gene="lepB"
                     /locus_tag="VEV43_13115"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYP24505.1"
                     /translation="MESRHAPPPPPPPPPPTEEEPVASDAPVVEEEAPKKKHSFWREL
                     PVLVVIAFVVALLIQTFLLQAFYIPSASMEPTLVEGDRVLVEKMKYRFGEPERGDVVV
                     FEKEFGGVALVHDDPWWEDIGSALKGLFGFPTDGTQDFIKRVIAVGGDTVEGREGRVY
                     VNGEAVDEPYLAEGTETSPFAAVKVPEGMIFVMGDNRGNSDDSRAFGPIDVDEVVGHA
                     FLLLWPPSDFDTL"
     gene            3178..4131
                     /gene="lepB"
                     /locus_tag="VEV43_13120"
     CDS             3178..4131
                     /gene="lepB"
                     /locus_tag="VEV43_13120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYP24506.1"
                     /translation="MPRVHEVAKQLGVTSKEVLACLEELGEARTTHASSVDDALAERV
                     KEALAPETDVEAEVAPEPEPAAEAEPEPEPETEAESEAEPEPALVGAKASPAPGRDGK
                     KKRSWLRHLAELPVLILIAFVIAIVIKTFLVQAFFIPSGSMIPTLHVGDRVLVEKISY
                     RFGGPQRGQVVVFEKDVFGETPDLPWYDDARNFVRELLGLPTGIEEDYIKRVVAIGGD
                     TIRYYGQPRRMYVNGTEVPQDYVNRGQDQASPTITSQDCKRLQMQTEGGGCVVPAGKV
                     FVMGDNRGNSEDSRILGPIDEDKIVGHAFMVIWPPKDFGTL"
BASE COUNT          694 a         1390 c         1426 g          647 t
ORIGIN      
        1 gccgatcatg gcgaccttgc cggtcgacca cttctggtcg gcgacccact cgacggagtc
       61 gtagccggcc ttcttctcgc cgtctgcgcc gtagtcgtag cagccgccgg agtcaccggt
      121 gccgggcacg tcgacccaca cgcggtggta gcccttggcg acccagcggt cggcgtcgcc
      181 gttgcgcccg agtgccgagt agggcgacat ggtcatgatc gccggtgcct tgacgatctt
      241 gccgttcaag gtggcgtgga cgacctcggc gttgagagtc ccggccctcg tctcgatctt
      301 gtagacgacg gtctcggcga cgtaagaagg agcagcccgc gatggcgtcc ccaatccggc
      361 gacgagcaac gcgaccagtg ctgcgagcga cttcggtctc atggagtgtc cacccccttt
      421 gaccagtcag tacggaacgg cgcatttagg gtgtcgcgat ggctaccgtg aaaacccggc
      481 tggagcccgc gcccagcggc tcgatccacg tcggcaacgc ccgcaccggg ctcttctgct
      541 ggctgttcgc caagcaccac gggggcagct tcgtcctccg catcgcggac accgacgcga
      601 agcgggtgac cgaggagaac taccaggcgg tcctcgaaga cctccgctgg ctcggtctcg
      661 aatgggacga gggccccgag atcggtggcc ccaacgggcc gtatcgccag agcgagcgct
      721 tccacctgta taccgagaag gcgaacgagc tcctcgagaa ggggttcgcc tacccctgct
      781 actgcacgcc cgaggagctc gagctcgaac gcaagaccgc gcacgaagag agccgggccc
      841 ccggctactc gggccggtgt cgcaacctca ccgacgacga gcggcgggcg ttcgaggccg
      901 aggggcgcaa gccgtcgctg cgtttcgccg tcccggccga tcgcacgatc acggtcgagg
      961 acgcggtgct cggtgagctc tcgaccgacc tgggccgcac ccccgacttc gtcatccagc
     1021 ggtcggacgg ctcgcctacc tacatgctcg cggtgacggt cgacgacgtc gccatgggga
     1081 tcacgcacgt gatccgcggc aacgacctag tcgcgtcgac cgcgcggcag ctcctgctgc
     1141 gcgaggcctt cggcgtcgag gagatgccgg tgttcgcgca cctccctctg ctggtctcga
     1201 ccgagggcaa gccgctgtcg aagcgatggg gcgacgtctc ggtggcggct tatcgcgagc
     1261 agggcttcct gcccgaggcg atggtcaact acctcgcgct gctcggctgg tcctacgacg
     1321 acaagacgaa cgtcttctcg gtggacgagc tcgtcgagcg gttctcgctc gaacgcgtcg
     1381 gcaagaaccc cgcggccttc gacgtggcga agctcgagtg gctcaacggg cactacgtcc
     1441 gagcgctgac gccggccgag ctcgccgacc gcctcgttcc cttctgcgag cgcgccgggc
     1501 tggcggcgga ctcaccggaa gggcgcgaga tcctgaccgg cgtcgcaccg ctgataagcg
     1561 agcgcctcaa gcggctcgac gaggccccgc cgatgatccg cttcctgttc gaggacgtgg
     1621 agccggaggg caaggcagcg aaggcgctcg acggccagga cgactacctt gccgcggtcg
     1681 ccggcggcct cgcggcgctg gacaagtgga cgacgcccgc gatcgaggag gtgctgcgcg
     1741 gcctcgccga ggagcggggg ctcaagccca agcaggcgtt ccagccggtg cgcgcggcgg
     1801 tcaccgggac gctggtgtcg ccgccgttgt tcgagagcat cgagctattg gggcgggaga
     1861 ggaccctgag gcgcctgggg tatcctgcgt agccgcaatt accgcttgat ggggcgtggg
     1921 gtaacctggc agcccgtcgg attctggttc cgatagtcct ggttcgaatc caggcgcccc
     1981 agcgctagga agcaacaaaa acccggcccg gtagtctagt tggccaagga cgccgccctc
     2041 tcaaggcgga gatcacgggt tcgaatcccg tccgggctac gagtacgcgt cagtgaagag
     2101 caaggggaga acgatatgaa ccggacggac ctggtcgaga ggccgtacct gcgggacgac
     2161 ctgccgacgt tccgcgccgg tgacaccgtg aaggtgcacg tgcgcgtcgt cgagggcgag
     2221 cgtgagcgca tccaggtctt cgagggcatc gtgatccgcc ggcgcggcag ccagctctcc
     2281 gagaccttca ccgtccgcaa gatcagcttc ggcgtcgggg tcgagaggac gttccccgtc
     2341 cactcgccga tgatcgccaa gatcgaggtc ggggcccgcg gtgacgtccg ccgggccaag
     2401 ctctactacc tccgctcccg tgtcgggaag aaggcgaagg taaaagagtt gcgggacagg
     2461 taaacctcct tcgtggagtc ccgacacgca ccacctcctc cgcctcctcc gcctcctccc
     2521 accgaggaag agccggtagc ttcagacgcg ccggtcgtcg aggaagaggc acccaagaag
     2581 aagcattcgt tctggcgcga gctccccgtc ctcgtcgtca tcgcgttcgt cgtcgcgctg
     2641 ctgatccaga cgttcttgct ccaggccttc tacatcccgt ccgcttcgat ggagccgact
     2701 ctcgtcgagg gcgaccgcgt gctcgtcgag aagatgaaat accgcttcgg cgagcccgag
     2761 cggggtgacg tcgtcgtctt cgagaaggag ttcgggggcg tggccctcgt ccacgacgac
     2821 ccctggtggg aggacatcgg cagcgccctc aagggtctgt tcgggttccc gaccgacggg
     2881 acgcaggact tcatcaagcg cgtgatcgcc gtcggggggg acacggtcga aggccgcgag
     2941 ggccgcgtgt acgtcaacgg cgaggcggtc gacgagccct atctcgccga agggacagag
     3001 acatcgccgt tcgcggccgt caaggtgccg gaggggatga tcttcgtcat gggcgacaac
     3061 cgcgggaact cggacgactc ccgggcattc ggcccgatcg acgtcgacga ggtcgtcggc
     3121 cacgcgttcc tgttgctctg gcccccgtct gatttcgaca cgctctaacc tcgcgcgatg
     3181 ccgcgtgtgc acgaggtcgc caagcagcta ggcgtgacct ccaaggaggt cctggcgtgt
     3241 ctcgaggagc tgggcgaggc gcgcaccacc catgcgtcct cggtcgacga cgcgctcgcc
     3301 gagagggtga aggaggccct cgcgccggag acggacgtcg aagcggaggt tgcgcccgag
     3361 ccggagccgg ctgcggaggc cgagccggaa cctgaacccg agacagaggc ggagtccgag
     3421 gccgagcccg aacccgccct cgtgggcgcc aaggcctctc ccgctccggg gcgggacggg
     3481 aagaagaagc gctcgtggct ccggcacctc gccgagctcc ccgtcctcat cctcatcgcg
     3541 ttcgtcatag cgatcgtgat caagacgttc ctcgtccagg cgttcttcat cccgtccggc
     3601 tcgatgatcc cgacgctcca cgtcggcgac cgcgtcctcg tcgagaagat ctcgtaccgc
     3661 ttcggcggcc cccagcgcgg ccaggtcgtc gtcttcgaga aggacgtctt cggcgagacc
     3721 ccggacctgc cctggtacga cgacgcgcgg aacttcgtgc gcgagctgct cggcctgccc
     3781 acggggatcg aggaggacta catcaagcgc gtcgtcgcga tcggcggcga cacgatccgc
     3841 tactacggcc agccgcgccg gatgtacgtg aacgggaccg aggtgccgca ggactacgtc
     3901 aaccgggggc aggaccaggc gagcccgacg atcacgtcgc aggattgcaa gcgcctccag
     3961 atgcagaccg aggggggagg ctgcgtcgtc ccggccggga aggtcttcgt gatgggcgac
     4021 aaccgcggga actccgagga ctcgcggatc ctcggcccga tcgacgagga caagatcgtc
     4081 gggcacgcgt tcatggtcat ctggccgccg aaggacttcg ggacgctcta gcatcgggcg
     4141 gatggtcatg gacgcca
//
LOCUS       DASQDO010000478        17737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_785028, whole genome shotgun sequence.
ACCESSION   DASQDO010000478 DASQDO010000000
VERSION     DASQDO010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17737
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785028"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VEV43_13125"
     CDS             <1..506
                     /locus_tag="VEV43_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HYP24507.1"
                     /translation="LETMAPKDFLEFRQHLAPASGFQSVQFREIEFISGLKDASYLDR
                     VSESPEERARLERRLAEPTLWDAFCTMLDARKLPMPPDDVEARRASLLKVARDREKYP
                     DEYYLSEALLEHDQHFATWRSRHVLMVERQIGTKSGTGGSTGAPYLRSTLNKRFYPEL
                     WDLRSYL"
     gene            complement(740..1546)
                     /locus_tag="VEV43_13130"
     CDS             complement(740..1546)
                     /locus_tag="VEV43_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP24508.1"
                     /translation="MPLADLGDVQLHYKDHGRPEAPPALGIMGFALDQRYWAAQIPAV
                     TATHRFVTFDNRGVGRSTGHATVPLETMADDTVRLLDHLGIEKTVVFGLSMGGTIAQH
                     VVLDHPDRVSALILGVTWARPLEFMRRQDDIGRVLVEARGPEWLMEIALLRMFTPKFF
                     EMGAEALDRMMRAMNAPGAPEGPTGETLLAQIDALTKHDVLGRLGEVTCPTLVVGGKM
                     DIMVPGFASEEIAAAIPGAELTMFETGHGCMIEEMADFNARISRFLAGLV"
     gene            1606..1923
                     /locus_tag="VEV43_13135"
     CDS             1606..1923
                     /locus_tag="VEV43_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24509.1"
                     /translation="MRKSLGVAAMVFVLLAPAGAAAAQEPQARDPFLPLVQPEPVPTD
                     GGTGVPTDPTVPVDPVDPTDPLPTTGSSTATWVGLGYVLIALGTGAVVLSKVLRPVRV
                     ASR"
     gene            1972..2283
                     /locus_tag="VEV43_13140"
     CDS             1972..2283
                     /locus_tag="VEV43_13140"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HYP24510.1"
                     /translation="MNPCSKPGCANPGAALLGYYYAERRAVLDDPPEGEISPHAYVLC
                     TKCAEKLTPPRGWVLEDNRAEPPLFLDRIVKERDEAAAVEKLPPPEEPRRQQLFFGYT
                     A"
     gene            2321..2617
                     /locus_tag="VEV43_13145"
     CDS             2321..2617
                     /locus_tag="VEV43_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24511.1"
                     /translation="MPFDKRLLGELRKLDDPDLRRVLIFVRGLLRSRGDEASEDFGSD
                     ETGPVTYRLESVKCGKPGCTKCPHGPYWYAYWREDGKVRSRYIGPELPTKESNA"
     gene            2685..2870
                     /locus_tag="VEV43_13150"
     CDS             2685..2870
                     /locus_tag="VEV43_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24512.1"
                     /translation="MRCPECGVEDTTEIEIHLKEDETVQFFSCRRCEARWWKRDGDTI
                     ALDEVLTIAARSKPQRK"
     gene            complement(3110..3796)
                     /locus_tag="VEV43_13155"
     CDS             complement(3110..3796)
                     /locus_tag="VEV43_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP24513.1"
                     /translation="MHDRRPGPLDRVRRTAARVRARGAGEILGVARGRVQEWVRSEDE
                     LIMLVREPSEPTPDFRHDPGLVFRGASPADGPLYAREIGTDSAATFGRRLTPTTNCFF
                     AFGGDELLHASWVTTGAAWTREIDAYVVPPPGDAYVYESFTRPEARGRGVYPFALRGI
                     CEWAAEADVPRVWVAVESGNAPSLKAIRKAGFEPAYEMRYGRRVGRRSLVVDKPEGLV
                     VPAVLRRVEG"
     gene            complement(3803..4072)
                     /locus_tag="VEV43_13160"
     CDS             complement(3803..4072)
                     /locus_tag="VEV43_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYP24514.1"
                     /translation="MNTSLPALDAEWRERAACLSFPSILFFGVDDNETPVERRGREER
                     AKEICAICPVRKECLDYALATKEPYGIWGGLTEVERKSRLRGRAS"
     gene            4138..5505
                     /locus_tag="VEV43_13165"
     CDS             4138..5505
                     /locus_tag="VEV43_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYP24515.1"
                     /translation="MTDLSAIFKAYDVRGTYPEQLDEALARRIGGAFATFVEGNDRSP
                     RRAAKGRRIVLGRDMRVSSPSLAAAFAEGVTAAGTDVVDVGEVSTDALYFASGRMDLP
                     GAMFTASHNPGHYNGIKLCRRAAAPIGAESGLQEIRALAEKGTAAAAEPGRVTEHDVL
                     TEYAAHCRTFVDTSALQPLKVAIDAANGMAGKTVPVVFRELPFELVPLYFELDGTFPN
                     HPANPIEPENLVDLQKAVTGEGCQAGIAFDGDADRMFLVDERGELVSGSTTTALVAER
                     LLRRHPGEAVIYNLICSWAVPEVIEENGGRPVRTRVGHSFIKQVMAETGAIFGGEHSG
                     HYYFRDNFRADSGMIAALVTLEAMSESGLPLSEMLRPYQRYAPSGEINSTVADQGAAL
                     AKLAEAYRDGRQDTSDGLTVEFGDWWFNCRPSNTEPLLRLNLEARDEALMAAKRDEVL
                     SLIRS"
     gene            5627..6508
                     /locus_tag="VEV43_13170"
     CDS             5627..6508
                     /locus_tag="VEV43_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HYP24516.1"
                     /translation="MGDIGKRLTAPATLILLALIVSVILVPVAASAHHKDGHQTGKGQ
                     SSGSSTVPVPTAGTGDHDGDADLDPVTTIEDSHEEEVGVDDNRHPSGKDRSAEHGGSG
                     NQGKSESAPDDNVGPMRNECGASPASDPRGGCTDKPGGSGGMDLHDQDGNNGCGNDDD
                     FDDDNNGHCGGKAKDRGAPPAPTSPPPAKPETPPSKPETPPTNPPGKNPEGPKVKPPI
                     VEPVPDTPGEPRVKRRQITSEEPPVRVDNRGPAPVPAAQLPYTGPEDTLAFVMFGFAL
                     LLLGGGMVQLVSVRPTL"
     gene            6623..6799
                     /locus_tag="VEV43_13175"
     CDS             6623..6799
                     /locus_tag="VEV43_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HYP24517.1"
                     /translation="MTLDARLLEILVCPNCRGDVEYREGDALLVCRGECGYRYPIRDG
                     IPVLLVDEAEKPGA"
     gene            6838..7434
                     /locus_tag="VEV43_13180"
     CDS             6838..7434
                     /locus_tag="VEV43_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYP24518.1"
                     /translation="MLLLDLAGPPRCATCGVLGHVLCARCRSELVVCPPLDPLPPLAR
                     LSAACLYEGAARSLVLGLKLGALRTRAGPLADAMCASAWRDGIAGNVVTWVPGNPVEA
                     RRRGYDHGEVLARLVAARLGMPVTSLLVPAGAKKDQTTLSGAQRRENPKGAFVARTVH
                     GRVVIVDDVMTTGATICACAGALRRAGAEVEGLVGCRA"
     gene            7561..8247
                     /locus_tag="VEV43_13185"
     CDS             7561..8247
                     /locus_tag="VEV43_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP24519.1"
                     /translation="MISEDAIRVLIVDDHALFRRGLQMVLEGEADIDVVGEASDGHEA
                     IERAEATTPDVVLMDVRMPKRSGIEATRTIKDTLPSTKILMLTISDEEADLYEAIKAG
                     ASGYLLKEISIEEVASAVRQVQAGQSLISPSMASKLLNEFAAMVKRRDERTQVPGPRL
                     TERELEVLKLVAKGMNNRDIGAELFISENTVKNHVRNILEKLHLHSRMEAVVYAVREK
                     LLDIKELPGG"
     gene            complement(8512..9549)
                     /locus_tag="VEV43_13190"
     CDS             complement(8512..9549)
                     /locus_tag="VEV43_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24520.1"
                     /translation="MNALLRLHETVDGTRSWDRLPGYMKAGTHREEVIKKQTYAGKRI
                     RQGCRWISTTAYLALRASGLTEDRIVEVQATIYPPDDPEHATLYISANTKTAIAALEK
                     LFASGTAGAALDAMIALAQTMSGTEQNSLGDRVTRHPSKLQGLSARNDPADPDAHAWR
                     VLQAALKRRAVVARRASDDDGPSGADPHAELNLRDEINVRQERTDIELDPAHVGGLRR
                     PCVQCHEKLYRHAGASPTRPGRLWLTQAASVGIKALGSTPDEIALHIFKNVHTTYLTY
                     REGGKPTDRVNTSSSSEAEPVPTIARPYSGPAEPWNMPFRDETAPPAPVPPLQERFVE
                     EDPGYWADSDQ"
     gene            10185..12947
                     /gene="secA"
                     /locus_tag="VEV43_13195"
     CDS             10185..12947
                     /gene="secA"
                     /locus_tag="VEV43_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HYP24521.1"
                     /translation="MSIIKKVLAAGEGKKLKALQAIVPRVNSLEDEVKRLDDDALRAR
                     TGEFKQRVERGESLDDLLPEAFATVREGAMRVIGQRHFDVQLMGGGALHQGWIAEMKT
                     GEGKTLTATLAVYLNALEGKGVHVVTVNDYLAKRDSEWMGQVYRFLGMQTGLIQSQMS
                     PQERLPAYAADITYGTNNEFGFDYLRDNMVTEYERMVQRGHHFATVDEVDSILVDEAR
                     TPLILSGAVQENNKWYQQFARISPRLERDTHYEVDEKKHTVAVTEEGVAKVEEILGVD
                     NLYDHVNVDLVHHLQASLKAKELYKLDVQYVVKDGEVLIVDEFTGRILPGRRYSEGLH
                     QAIEAKEGVRIKEENQTLATITLQNYFRMYDKIAGMTGTAQTEAAEFGHIYKLEVAPI
                     PTNRPMVRKDEQDLIYKTAEAKWNSVADDVAKRYEAGQPVLIGTVSIEKSELLSRILQ
                     KRGVPHSVLNAKHHEKEASIVALAGRLHAVTVETNIAGRGVDMLLAGNAEGMARAEMQ
                     AAGWDNDAYLLAEYPPEEMAEYESQFNPLFERYKTQCAAEHEKVVAVGGLYVLGTERH
                     DSRRIDNQLRGRAGRQGDPGESRFYLSLEDDLMRVFASDRIAGMMERLKIPDDVPIEA
                     KIVSKAIERAQTQVEGMNFEIRKNVLKYDEVMDKQRQVIYAERRKILEGADIGDQAFD
                     LVTDVVEAAVAENANPELPPEEWDWEALFHRAREIFPTSLTIEGFDETTVTYEEVQSA
                     FLEDAAKVYQQREEELGEEQMRKIERLVLLSVIDNRWREHLYEMDYLQEGIGLRAMAQ
                     KDPLVEYQREGFDMFLTMQESIKEDFARYVFHVEVVREDEQRRNEPTRVREERRQIPM
                     ASMAAAPPPPAEAGNGRAAEAADAQAVAIEQAVSDKIPRNAPCPCGSGKKYKQCHGRP
                     GVADLG"
     gene            13181..>17737
                     /locus_tag="VEV43_13200"
     CDS             13181..>17737
                     /locus_tag="VEV43_13200"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HYP24522.1"
                     /translation="MNPAVATAAIGLALAAGSFGISAFSTSAIAPGARQIPAGCHDDD
                     AIVGTPEDDKLKGTKFDDVIFGGGGDDVIKGKGGNDLICARGGDDFVKGGNGNDTIYG
                     GGGSDELRGGPGDDTIRGGPGTDTANGGADTDDCEAESETNCEEPPTPTPTPTATPTP
                     TASPTATPTTTPSPTPTPTNQPKNLKPNAANDSYATDEDQSLSVPAPGVLANDSDPEN
                     NSITAMLVTNAVNGDVTLNPNGSFQYTPDPDFNGPDTFTYKASDGTNLSDTATVTVNV
                     TAMPDAPDGIAQNVSTNEDAFNVVSLFGTDPDGTTPSVFKITAVPTNGDLRNGSGLGG
                     QLIGAGDLPFTLSGNQVTYVPDLNFFGNDSFDFKANDGSLDSGPAPVEIDVLPMNDPP
                     TDISLSNNSVEEEQAVGTAVGNLTAADGDAGDTHTFSLAAGAGDADNAKFTIAGGVLK
                     TAQSFDFETQGPFSIRVRATDAGNAPFEKQFAVTLVNANDQPAVTGGTFSLPENSANG
                     AAVGSPSVSDQDAGQTHTWSITGGNSGSAFAIDSATGAITVADTGELDFEDVQQWVLT
                     VKAADDGSPAKFDTDTVTINLTNVNEAPTDITLDNASVAENLASGTNVGNLAPIDPDA
                     NDTHTFSLVAGAGDGDNGKFQITNGVLQTAQSFDFETQGPFSIRVRARDAGNLDYEEV
                     FSISVTNVNDAPVLGAATYALDENSANVTAVGTPSATDEDSPAQNLTYSITGGNTLGA
                     FQINSNTGAITVADATDLDFETNPTFALTVQVADDGTPARDDSETITVNLNNLNEKPV
                     VNAATLSLPENSANGTSVGTVTSSDPDAGQTVTYAITGGNTGNAFAINSNTGEITVAD
                     ANDVDFESNHPFSLTVEGTDNGAPILTDTETITVNLTNVNEAPTDISLNDTDVAENAS
                     LNTVVGTISGSDPDAGDSGTLVFSLASGAGDTDNARFNVGPSNSLRTSEVFDKETQGP
                     FSVRLRATDGGTLQYEEAFQISVVDVNEAPDVNAATFAIDENSANGTAVGTATATDVD
                     AGQTLTWAITGGNTGGAFQINPNTGAITVADVTDVDRETNHPFSLTVQATDNGSPALS
                     DTDTVTVNLNDLNDNPVVNAATLAVAENSANGTSVGTATATDQDNPAQTLTWAITGGN
                     ASGAFAIDSGTGAITVADTGDLDFETTPAFNLTVEATDNGTPNRTGSNAVTVNLTNVN
                     EKPAITAPTTISAQYGVAKALSGISVSDPDSGNADISMTLAVLDGTLDLDDSVANGLA
                     AVDITGNGTDALVLTGKQSEINNTLGAATGLTYTSDAGFLGLSDTLAIATNDGGNTGS
                     GAAQVDAANVTIQFNTPPVATDQTVSTNEDTEKTVTLAGTDADADPLSFKITSLPAAG
                     KLHEGTSSAGTEITAGALPYTLPADKVTYVPPLNQNGLSLETFAFKANDGSTDSAAGV
                     VTVDVTAVDDISVAENDAFSVDEDSGATALLVRTNDTDVDGAVELIASVTQPANGTVT
                     KHLGDTRVQYTPNAHYCNNPPATPV"
BASE COUNT         3206 a         6079 c         5897 g         2555 t
ORIGIN      
        1 tgctggagac gatggccccg aaggacttcc tcgagttccg ccagcacctc gcccccgcca
       61 gcggcttcca gtccgtgcag ttccgcgaga tcgagttcat ctcaggcctc aaggacgcca
      121 gctacctcga ccgggtctcg gagagcccgg aggagcgtgc ccggctcgag cggaggctcg
      181 ccgagccgac cctgtgggac gcgttctgca cgatgctcga cgcacggaag ctcccgatgc
      241 cccccgacga cgtcgaggcg cggcgcgcgt cgctgctgaa ggtcgcccgc gaccgggaga
      301 agtaccccga cgagtactac ctgtccgagg cgctgctcga gcacgaccag cacttcgcca
      361 cgtggcggag ccggcacgtg ctgatggtcg agcggcagat agggacgaag agcgggacgg
      421 gcggctccac gggggccccg tacctgcgat cgacgctgaa caagcgcttc tatccggagc
      481 tgtgggacct gcggagctat ctatagcgcc tgcggcaaat gtcgcgaaga aatatcggcc
      541 gactaccttg cgtagtgacc ccgggccgtc gtaagatgtt caggtacctc gcagagataa
      601 gtaggtctcg gccgaaagtc actgcggggt acattcgcgt ccggggcctg gtcagggccc
      661 ctttttcgcg cccgcggcga accctcgagc acagatcgag gccgggacgt attacaaaac
      721 tttcttgggt acgaacccgt tacacaagac cagccaggaa gcgggagatc cgcgcgttga
      781 agtcggccat ctcctcgatc atgcagccgt ggccggtctc gaacatcgtc agctcggccc
      841 ccgggatcgc cgcggctatc tcctccgaag cgaagcccgg caccatgatg tccatcttcc
      901 cgccgacgac gagggtcggg caggtcacct cgcccagccg gccgaggacg tcgtgcttcg
      961 tcagcgcgtc gatctgggcc aggagcgtct cgcccgtcgg gccctcggga gcgcccggag
     1021 cgttcatcgc ccgcatcatc cggtcgaggg cctcggctcc catctcgaag aacttcgggg
     1081 tgaacatccg gaggagcgcg atctccatga gccactcggg tccccgcgcc tcgacgagga
     1141 ctcgcccgat gtcgtcctgc cggcgcatga actcgagggg acgggcccac gtcaccccga
     1201 ggatcagagc cgagacccga tcggggtggt cgagcacgac gtgctgggcg atcgtcccgc
     1261 ccatcgagag cccgaacacg acggtcttct cgatgccgag gtggtcgagg agcctgacgg
     1321 tgtcgtcggc catcgtctcg agcggcacgg tcgcgtggcc ggtcgagcgg cctacgcccc
     1381 ggttgtcgaa cgtcacgaac cggtgggtgg cggtgacggc cgggatctgc gcggcccagt
     1441 agcgctggtc gagggcgaag cccatgatcc cgagagccgg aggcgcctcg gggcgaccgt
     1501 ggtctttgta gtggagctgg acgtctccga ggtcggcgag cggcatcggc cgatcttagg
     1561 ccccggctct ggcgcgtaaa tgccgccgcg ccgtaatctg gatacgtgag gaagtccctg
     1621 ggcgtcgcag cgatggtctt cgtgctcctg gctcccgcgg gggccgcggc cgcgcaggag
     1681 ccccaggccc gcgacccctt cctcccgctc gtccagcccg agccggtccc gaccgacggc
     1741 gggacggggg tgccgacgga cccgaccgtc ccggtcgatc ctgtcgaccc gaccgacccg
     1801 ctcccgacga cgggatcctc gacggcgacg tgggtgggcc tcggctacgt cctgatcgcg
     1861 ctcggcaccg gcgcggtcgt cctgtcgaag gtcctccgcc cggtccgcgt cgcctcgcgc
     1921 tagccctgca atccgcggaa tcccggggcc gctcccgtag tcttctgcgg cgtgaacccc
     1981 tgctcgaagc ccggctgcgc caacccgggg gccgcgctcc tcggctacta ctacgccgag
     2041 cgccgcgcgg tcctggacga ccccccggaa ggcgagatct cgccccacgc gtacgtcctc
     2101 tgcacgaagt gcgcggagaa gctgacgccg ccgcggggat gggtgctcga ggacaacagg
     2161 gccgagcctc cgctgttcct cgaccgcatc gtgaaggagc gcgacgaggc ggcggccgtc
     2221 gagaagctcc cgccgccgga ggagccgcgc cgccagcagc tgttcttcgg ctacaccgcg
     2281 taagggcgcg gatcggccgg ccgggggatg atctcgggac atgcctttcg acaaacgtct
     2341 gctgggcgag ctccgcaagc tcgacgaccc cgacctgcgc cgggtcctga tcttcgtgag
     2401 gggcctgctc cggtcgaggg gcgacgaggc gagcgaggac ttcggctcgg acgagaccgg
     2461 cccggtgacg tatcggctcg agtcggtcaa gtgcgggaag cccggctgca cgaagtgccc
     2521 ccacggcccc tactggtacg cgtactggcg agaggacgga aaggtccgga gccgctacat
     2581 cgggccggag ctaccgacga aggaatccaa cgcgtagggg tttcgttttc gagcgagttg
     2641 gaacaaaatg gaagcagcag gacaccggca agggagagcc agcgatgaga tgccctgagt
     2701 gcggcgtaga ggacacgacc gagatcgaga tccacctgaa ggaagacgag acggtgcagt
     2761 tcttctcgtg ccggcgctgc gaagcccgct ggtggaagcg agacggcgac acgatagccc
     2821 tggacgaagt actgaccatc gcggccagga gcaagcccca acgcaagtaa ccgaaccggt
     2881 cgggcgtttg cggaggagta tgcgggcgct cgcggccttg gtccgtcccg gcaacaggtg
     2941 ccagttgagg gggtcgtgcc ggtagcgtgg ccctggggct ggaattgctc ccaaccgcag
     3001 gacgccgtct cggtcctggc cctcgagcgc ccgcaaacct ttatcgctta cggagcgtag
     3061 ccccgcgagt atggcgtgct cgaattgatt tggctacccc ccacaggggc tagccctcga
     3121 cccgcctgag gacggccggc accaccaggc cctccggttt gtcgacgacg aggcttcgcc
     3181 gccccactcg gcggccgtag cgcatctcgt acgcgggctc gaaccccgct ttgcggatcg
     3241 ccttcagcga cggtgcgttg ccggactcga ccgcgaccca gacccgcggg acgtccgcct
     3301 cggccgccca ctcgcagatc cctcggagcg cgaacgggta tacgccccgg cccctggcct
     3361 cgggccgcgt gaacgactcg tacacgtagg cgtcgcccgg gggcgggacg acgtaggcgt
     3421 cgatctcgcg cgtccacgcg gcccccgtcg tgacccagct cgcgtgcagg agctcgtcgc
     3481 cgccgaaggc gaagaagcaa ttcgtggtcg gcgtcagccg ccgcccgaac gtcgcggcgg
     3541 agtcggtccc gatctcccgc gcgtagaggg ggccgtcggc gggggaggcg ccccggaaga
     3601 cgaggccggg gtcgtggcgg aagtccgggg tcggctcgga gggctcccgc acgagcatga
     3661 tcagctcgtc ctcggagcgg acccactcct gcacccggcc ccgggcgacg ccgaggatct
     3721 cgcccgcccc ccgcgcccgg acgcgcgcag ccgttcgccg gacgcggtcg agcgggccgg
     3781 gccggcggtc gtgcaactca ggtcagctgg cgcggccgcg caggcggctc ttgcgctcga
     3841 cctcggtaag ccctccccag atcccgtacg gctccttcgt cgccagcgcg tagtcgaggc
     3901 actccttccg caccgggcag atcgcgcaga tctccttggc ccgctcctcg cggccgcggc
     3961 gctcgacggg cgtctcgttg tcgtcgacgc cgaagaacag gatcgacggg aacgagaggc
     4021 aggcggcgcg ctctcgccat tccgcgtcga gcgccggcag ggaagtgttc atctgagaga
     4081 aatggttcca gcccgggtgg cggtcgtcaa cgttctccgg ctcttaagat gcgcgccgtg
     4141 accgacctct cggcgatctt caaggcctac gacgtccgcg ggacgtaccc ggagcagctc
     4201 gacgaggcgc tcgctcgccg gatcggcggc gcgttcgcga cgttcgtcga gggcaacgac
     4261 cggtcgcctc ggcgagcggc caagggcaga cggatcgtcc tcgggcgcga catgcgggtg
     4321 tcgtccccgt cgctcgcggc ggccttcgca gagggcgtca cggccgccgg gaccgacgtg
     4381 gtcgacgtcg gcgaggtctc gaccgacgcg ctctacttcg cctcgggacg gatggacctc
     4441 cccggcgcga tgttcacggc gtcgcacaac cccggccact acaacgggat caagctgtgc
     4501 cgccgggccg cggcccccat aggcgcggag tccggcctcc aggagatcag ggccctggcc
     4561 gagaagggca ccgccgccgc ggcggagccc ggccgggtca ccgagcacga cgtcctcacc
     4621 gagtacgcgg cgcactgccg gaccttcgtc gacacgtcgg cgctccagcc cttgaaggtc
     4681 gccatagacg cggcgaacgg catggccggc aagaccgtgc cggtcgtgtt ccgcgagctc
     4741 ccgttcgagc tcgtgccgct ctacttcgag ctcgacggga cgttcccgaa ccacccggcg
     4801 aacccgatcg agcccgagaa cctcgtcgac ctccagaagg ccgtgaccgg cgagggctgc
     4861 caagccggga tcgcgttcga cggcgacgcg gaccggatgt tcctcgtcga cgagaggggc
     4921 gagctcgtgt ccggctcgac cacgaccgcg ctcgtcgcgg agcggctgct ccgccggcac
     4981 ccgggcgaag ccgtgatcta caacctcatc tgctcgtggg ccgtgcccga ggtcatcgag
     5041 gagaacggcg gccgtcccgt acgcacccgc gtcgggcact cgttcatcaa gcaggtcatg
     5101 gccgagaccg gcgcgatctt cggcggcgag cactccggcc actactactt ccgcgacaac
     5161 ttccgggcgg actccgggat gatcgcggcc ctcgtgacgc tcgaggcgat gagcgagtcg
     5221 ggcctcccgc tctccgagat gctgcgcccc taccagcgct acgcgccgtc gggcgagatc
     5281 aactcgaccg tcgccgacca gggggccgcg ctggcgaagc tcgccgaggc ctaccgggac
     5341 ggccggcagg acacgagcga cggcctgacc gtcgagttcg gggactggtg gttcaactgc
     5401 cggccgtcga acaccgagcc gctgctgagg ctcaacctcg aggcccgcga cgaggccctg
     5461 atggccgcta aacgcgacga ggtcctctcc ctgatccgct cgtaacgcca caaatcccca
     5521 ggaagcgggg cggatttgta ttttctccca attttggaga aggtctcccc tgagcggaga
     5581 ggagttgccg aaccaaatga aggagatcgt tcgaccaact taggaggtgg gagacatagg
     5641 aaagcgcctg acggcgccgg caaccctcat cctcctcgct ctgatcgtct cggtcatcct
     5701 cgtgcctgta gccgcttcgg ctcatcacaa ggacggccac cagactggca agggccagtc
     5761 gtccggcagc agcacggtcc cggtgcccac cgcgggcacg ggcgaccacg acggggacgc
     5821 cgacctggat cccgtcacga ccatcgagga ctcgcatgag gaagaggttg gagtcgacga
     5881 caaccggcat ccctcgggca aggaccgctc ggcagagcac ggcggttccg gcaaccaggg
     5941 gaagtctgag tctgctcccg acgacaacgt cggtcccatg cgcaacgagt gcggcgcaag
     6001 ccccgcgagc gaccctcgcg gtggatgcac cgacaagcct ggtggtagcg gcggaatgga
     6061 cctgcacgac caggacggca acaacggctg cggcaacgac gacgatttcg acgacgacaa
     6121 caacggccac tgcggcggga aggccaagga ccggggagca cctccggcgc ccacgagccc
     6181 tccgcccgca aagcccgaga caccgccgag caagccggag actccgccta cgaacccccc
     6241 gggcaagaac ccggagggac cgaaggtcaa gccgccgatc gtcgagcccg tgcccgacac
     6301 cccgggcgag cctcgggtca agagacgtca gataacgagt gaggaaccgc cggtgcgagt
     6361 cgacaatcga ggccctgcac cggtacctgc ggcgcaactt ccgtacacgg gaccggagga
     6421 cactctggct ttcgtgatgt tcggtttcgc cctgctgctt ctcggcgggg gcatggtgca
     6481 actggtgtcg gtgagaccga cgctgtgaga acgacgaatc acctagcgaa gcgacgcaca
     6541 gcggcgatcc tctcgaggac cgccggggac agatgggggc cgggagaccg gccccctgtt
     6601 cttttgcgtc acaatcttgc gtatgacgct cgacgcccga ctgctggaga tcctcgtgtg
     6661 cccgaactgc cgcggcgacg tcgagtaccg cgagggcgac gctctcctcg tgtgccgcgg
     6721 cgaatgcggg taccgctatc cgatccgcga cggcatcccg gtgctgttgg tcgacgaggc
     6781 cgagaagccg ggggcctagc tctctttcct gtcgtacccc gtgcgtagcg tcggggcatg
     6841 ctcttgctcg acctcgcggg acctcctcga tgcgctacgt gtggcgtcct cggccacgtc
     6901 ctctgtgcgc gctgccggag tgagctcgtc gtgtgcccgc cgctggaccc gcttcctcca
     6961 ctcgcgcggc tgtcggccgc gtgcctgtac gagggcgcgg cccgatcgct cgtgctcggg
     7021 ctcaagctcg gcgcgctgcg gacgcgggcc ggaccgctcg cggacgcgat gtgcgccagc
     7081 gcgtggcggg acgggatcgc gggcaacgtc gtgacctggg tgccggggaa tcccgtcgag
     7141 gcacgccgtc gcggctacga ccacggcgag gtcctcgcgc ggctcgtcgc cgcccggctg
     7201 gggatgccgg tgacgtcttt actggtcccg gcgggggcga agaaggacca gaccaccctc
     7261 tcgggtgccc agagaaggga gaacccgaag ggtgccttcg tggcccgtac cgtccatggc
     7321 cgggtggtca tcgttgacga tgtgatgact accggagcaa cgatttgtgc gtgtgcgggc
     7381 gctttgcgcc gggccggcgc tgaggtggag ggtctggtgg gctgccgggc atgagcaatg
     7441 gtgactacta ggcactattg cttagggcaa aatgcctggt caccggacta caatcccttc
     7501 aacgactagc cccgggctcc ggggtgggag ggggacgggt tgagcaagcc gccgggcaag
     7561 atgatcagcg aggacgcgat ccgggtcctg atcgtcgacg accacgcgct gttccgccgt
     7621 ggcctccaga tggtcctcga gggcgaggcc gacatcgacg tcgtcggcga ggcctccgac
     7681 gggcacgagg cgatcgagcg ggccgaggcg accacccccg acgtggtgct catggacgtc
     7741 cggatgccga agcgctccgg gatcgaggcc acgaggacga tcaaggacac gctcccctcg
     7801 acgaagatcc tcatgctgac catctccgac gaggaggccg acctctacga ggcgatcaag
     7861 gccggcgcct cgggctacct gctcaaggag atctcgatcg aagaggtggc gagcgcggta
     7921 cgccaggtcc aggccggcca gtccctgatc tcgccgtcga tggcgtcgaa gctcctgaac
     7981 gagttcgccg cgatggtcaa gcgccgcgac gagcgcaccc aggtcccggg cccccgcctg
     8041 accgagcgcg agctagaggt cctcaagctc gtcgcgaagg gcatgaacaa ccgcgacatc
     8101 ggggccgagc tgttcatctc ggagaacacg gtgaagaacc acgtgcgcaa catcctggag
     8161 aagctgcacc tgcactcgag gatggaggcc gtggtctacg cggtgagaga gaagctgctc
     8221 gacatcaagg agctccccgg tgggtaggtc gaactcctcc ggctcgtcga gccggggcgg
     8281 caagagctcg tcaggcggct ccaagtcgag cgggagctcg agttccggcg gtagcagcag
     8341 tggcggcacc agggcccaga aggccaaggc tggccgcaag ggaggcaggg cctcgagcga
     8401 caagagctag gcccactcgg ctacggaatc caccttcgac ttcggagaac gccggagcgg
     8461 tctggacaag cgtctgagac gacgaggtgg ggtcaattcc caggcctcag gttactggtc
     8521 cgagtcggcc caataacctg ggtcctcctc cacgaaccgt tcctggaggg ggggcactgg
     8581 agcaggaggc gctgtttcat cgcgaaacgg catgttccac ggttccgccg gaccagaata
     8641 cggacgggca atcgtcggca cgggctcggc ttcactggag gaagaggtat tgactctgtc
     8701 ggtgggcttt cccccctcgc ggtaagtcaa gtaagtcgtg tgaacgtttt tgaagatgtg
     8761 cagcgcaatc tcatctggcg tgctgccgag agcctttatg cctacggatg cggcctgggt
     8821 aagccacagc cttcccggtc gggtggggga ggcgcctgca tgtctgtaga gcttctcatg
     8881 gcattgcaca cagggccgtc gcagcccgcc aacgtgcgcg gggtccagct cgatgtcggt
     8941 tcgctcttgg cgcacattta tctcatcgcg gagattcagc tccgcgtgcg gatctgcacc
     9001 agagggtcca tcgtcgtcgg acgcgcggcg tgcgaccacg gctcgacgtt tgagtgcggc
     9061 ctgaagaacg cgccacgcgt gagcatcggg gtccgcaggg tcgtttctgg cggagagtcc
     9121 ttgcagcttg ctcgggtggc gcgtgacacg gtcgcctagg gagttctgtt cggtgcccga
     9181 catcgtctga gctaaggcga tcattgcgtc caatgcggcg ccggcggttc cactcgcaaa
     9241 cagcttttca agagccgcta tggcggtctt ggtgttcgct gagatgtaca aagtcgcgtg
     9301 ctcagggtca tctggtgggt agatcgtggc ttgcacctcg actatccgat cctcggtcag
     9361 acccgaggct cgcagggcaa ggtatgcagt cgtggagatc catcggcagc cctgtcttat
     9421 gcgtttcccg gcgtaggtct gtttcttgat cacttcttcc ctatgggttc cggccttcat
     9481 gtaaccgggc aggcgatccc aagacctcgt gccatcgacg gtctcgtgta gtcgcaggag
     9541 agcgttcatc acctggatga ggaattgact ccgggccgcc ttcgcggccg ggtccagcct
     9601 agggatgttg gacgctcggt ctttcgagga tttgctgccg ctcgctggac cccgaccgtg
     9661 ggccgcgccc gggggacgcc cgccgccaag gttcgcgctt ggttgccgag cgtggctcga
     9721 cgtgaagaaa ctccgaaaaa ttccgcccca agaggaccat tgccgggcgt ggatgccagg
     9781 tgtagccgaa gtcaaaggcc gtctcgcagc gggtccgaat ccgatatgag cgaggactct
     9841 tccgaatacc gaggggctcc gagcggctgc tgccaggcct gccgcggtga tgccgaccgc
     9901 cgctgcagcg ccccatgcgg aattcgtgat acgtggtgcc tgtgggagcg ggctccctcg
     9961 gggcacgccg acgggggtag agcgctccag ctcctcgacc ttggcgatcg tctcatcggg
    10021 catagtcata gatgtaacga atgacgaggg gtgccacaac gctcaaaggg gcagcgttgt
    10081 ctgccttttt gggaagcctc cagggccgct cgggaatcca tcgacgcgag gttgtttcgg
    10141 cggccggatg acttcgattc ggtcaggggg ataatgggcg gcctatgagc atcatcaaga
    10201 aggtccttgc cgcaggggag ggcaagaagc tcaaggccct ccaggccatc gttccccgcg
    10261 tcaactccct cgaggacgag gtcaagcgcc tcgacgacga cgccctccgc gcccgtacgg
    10321 gcgagttcaa gcagcgcgtc gagcgcggcg agtcgctcga cgacctcctc cccgaggcct
    10381 tcgccaccgt gcgcgagggc gccatgcgcg tgatcggcca gcgccacttc gacgtccagc
    10441 tcatgggcgg cggcgcgttg caccagggct ggatcgccga gatgaagacc ggcgagggca
    10501 agacgctgac cgctacgctc gccgtctacc tgaacgcgct cgaaggcaag ggcgtccacg
    10561 tcgtcaccgt caacgactac ctcgccaagc gcgactccga atggatgggc caggtctacc
    10621 ggttcctcgg tatgcagacc ggcctcatcc agtcccagat gtcgccccag gagcgcctcc
    10681 ccgcgtacgc ggccgacatc acgtacggga cgaacaacga gttcggcttc gactacctgc
    10741 gcgacaacat ggtcaccgag tacgagcgca tggtgcagcg cggccaccac ttcgcgacgg
    10801 tcgacgaggt cgactcgatc ctcgtcgacg aggcccggac gccgctgatc ctctcgggtg
    10861 ccgtccagga gaacaacaag tggtaccagc agttcgcgcg catctcgccg cggctcgagc
    10921 gcgacacgca ctacgaggtc gacgagaaga agcacacggt cgccgtcacc gaggaaggcg
    10981 tggcgaaggt cgaggagatc ctcggcgtcg acaacctcta cgaccacgtc aacgtcgacc
    11041 tcgtccacca cctgcaggcg tctctgaagg cgaaggagct ctacaagctc gacgtccagt
    11101 acgtcgtcaa ggacggcgag gtcctgatcg tcgacgagtt cacgggccgc atcctcccgg
    11161 gccgccgcta ctccgagggc ctccaccagg ccatcgaggc gaaagagggc gtgcgcatca
    11221 aagaggagaa ccagacgctc gcgacgatca cgctccagaa ctacttccgc atgtacgaca
    11281 agatcgccgg catgaccggc acggcccaga ccgaggccgc cgagttcgga cacatctaca
    11341 agctcgaggt cgcgccgatc ccgacgaacc gtccgatggt ccgcaaggac gagcaagacc
    11401 tcatctacaa gaccgccgag gcgaagtgga actcggtcgc cgacgacgtc gcgaagcgct
    11461 acgaggcggg gcagcccgtc ctcatcggaa ccgtgtcgat cgagaagtcc gagctcctgt
    11521 cgcgcatcct gcagaagcgc ggcgtcccgc acagcgtcct caacgcgaag caccacgaga
    11581 aggaggccag catcgtcgcg ctggccggcc gcctccacgc ggtgacggtc gagacgaaca
    11641 ttgccgggcg cggggtcgac atgctgctcg ccgggaacgc cgagggcatg gcgcgcgccg
    11701 agatgcaggc cgcgggatgg gacaacgacg cgtacctcct cgcggagtac ccgccggagg
    11761 agatggccga gtacgagtcc cagttcaacc cgctcttcga gcgctacaag acgcagtgcg
    11821 cggccgagca cgagaaggtc gtcgccgtgg gcgggctgta cgtcctcggc accgagcgtc
    11881 acgactcgcg ccgcatcgac aaccagctgc gcggccgtgc aggacgccag ggagaccccg
    11941 gcgagagccg cttctacctc tcgctcgagg acgacctcat gcgggtgttc gcgtcggacc
    12001 gcatcgccgg gatgatggag cggctcaaga tccccgacga cgtccccatc gaggccaaga
    12061 tcgtcagcaa ggccatcgag cgcgcgcaga cgcaggtcga gggcatgaac ttcgagatcc
    12121 gcaagaacgt cctcaagtac gacgaggtca tggacaagca gcgccaggtc atctacgccg
    12181 agcgtcgcaa gatcctcgaa ggcgcggaca tcggcgacca ggccttcgac ctcgtgaccg
    12241 acgtcgtcga ggccgcagtc gcggagaacg ccaaccccga gctcccgccc gaggaatggg
    12301 actgggaggc gctgttccac cgcgcgcgcg agatcttccc cacgtctttg acgatcgagg
    12361 gcttcgacga gaccaccgtc acgtacgagg aggtccagag cgccttcctc gaggacgcgg
    12421 cgaaggtgta ccagcagcgc gaggaggagc tcggcgagga gcagatgcgc aagatcgagc
    12481 gcctcgtcct cctgtcggtg atcgacaacc ggtggcgcga gcacttgtac gagatggact
    12541 atctgcaaga aggcatcggg ctgcgcgcga tggcgcagaa ggacccgctc gtcgagtacc
    12601 agcgcgaggg gttcgacatg ttcttgacga tgcaggagag catcaaggag gacttcgccc
    12661 gctacgtctt ccacgtcgag gtcgtgcgcg aggacgaaca gagaaggaac gagcccacgc
    12721 gcgtccgcga ggagcggcgg cagatcccga tggcgtcgat ggcggccgca ccgcccccgc
    12781 cggccgaggc cggcaacggt cgcgcggcgg aggcggccga cgcccaggcg gtcgctatcg
    12841 aacaggcggt ctcggacaag atcccccgca acgccccctg cccctgcggg agcggcaaga
    12901 agtacaagca gtgccacggc cgccccggcg tggccgacct cggctgagac gcgcacctct
    12961 tcgcggacta gtgaacgggg tacccaaagg actatctgtc gcggccggat gtaactaaag
    13021 aagcgcagta gttccacgca gcgccccttc agccggcctc gcgccgggcc gatgaccccc
    13081 tgagcggcac accggcggaa tagcgaaaag gggccattgt ccaatgcgaa acagcctgtt
    13141 tggcttcgaa ttaacctttt atgaaaggcg ggcgtttcgc atgaatccag ccgtcgccac
    13201 agcggcaata ggtcttgccc ttgcggccgg gtccttcggg atctcggcct tttcgacgtc
    13261 tgcgatcgcg cccggggctc gtcagatccc ggcaggctgt cacgacgacg acgcgatcgt
    13321 cggcacgccc gaggacgaca agctgaaggg gacgaagttc gacgacgtca tcttcggcgg
    13381 cggcggcgac gacgtcatca agggcaaggg cggaaacgac ctcatctgcg cgcgcggcgg
    13441 agacgacttc gtcaagggcg gcaacggcaa cgacacgatc tacggcggcg ggggctccga
    13501 cgagctccgc ggcggccccg gcgacgacac gatcagaggc ggtccgggaa cggacaccgc
    13561 caacggcggc gccgacacgg acgactgcga ggccgagtcg gagacgaact gcgaggaacc
    13621 gcccacgccg acgcctactc cgactgcaac cccgacgccg accgcgtccc ccacggcgac
    13681 cccgaccacg accccttcgc ccacgccgac gccgacgaac cagcctaaga acctgaagcc
    13741 caacgcggcg aacgacagtt acgccacgga cgaggaccag tcgctgagcg tcccggcccc
    13801 cggcgtgctc gccaacgaca gcgaccccga gaacaactcg atcacggcga tgctcgtgac
    13861 gaacgccgtc aacggtgacg tcacgctgaa cccgaacggg tcattccagt acacgcccga
    13921 cccggacttc aacgggccgg acacgttcac ctacaaggct tccgacggga cgaacctcag
    13981 cgacaccgcg accgtgaccg tcaacgtcac ggcgatgccc gacgcacccg acgggatcgc
    14041 gcagaacgtc tccacgaacg aagacgcctt caacgtcgtg tcgctgttcg gcaccgaccc
    14101 cgacggcacc acgccctcgg tcttcaagat caccgcggtc ccgacgaacg gcgaccttcg
    14161 caacggctcg gggctcggcg gccagctcat cggcgccggc gacctcccgt tcacgctctc
    14221 gggcaaccag gtcacgtacg tcccggacct gaacttcttc gggaacgact cgttcgactt
    14281 caaggcgaac gacggctctc tcgactccgg accggccccg gtcgagatcg acgtcctgcc
    14341 gatgaacgac cctccgaccg acatctcgct gtcgaacaac tccgtcgaag aggagcaggc
    14401 cgtcgggacg gcggtcggga acctcaccgc ggccgacggc gacgccggtg acacgcacac
    14461 gttctcgctg gcagcggggg ccggcgacgc cgacaacgcc aagttcacga tcgccggcgg
    14521 ggtgctgaag accgcccagt ccttcgactt cgagacccag ggaccgttct cgatccgggt
    14581 gagggccacg gacgccggga acgcaccgtt cgagaagcag ttcgccgtga cgctggtcaa
    14641 cgcgaacgac cagccggccg ttacgggcgg gactttctcg ctccccgaga acagcgccaa
    14701 cggggcggcc gtcggcagtc cttccgtttc cgatcaggac gcgggtcaga cccacacgtg
    14761 gtcgatcacc ggcggcaact ccggcagcgc cttcgccatc gactcggcta ccggggcgat
    14821 caccgtcgcc gacacgggcg aactcgactt cgaggacgtc cagcagtggg tgctgaccgt
    14881 gaaggcggca gacgatgggt cgcccgccaa gttcgacacg gacacggtca cgatcaacct
    14941 gacgaacgtc aacgaggctc cgaccgacat cacgctcgac aacgcctcgg tcgcggagaa
    15001 cctggcctcg gggacgaacg tggggaacct cgcgccgatc gaccccgacg cgaacgacac
    15061 gcacacgttc agcctcgtcg ccggcgccgg cgacggtgac aacggcaagt tccagatcac
    15121 taacggtgtc ctgcagacgg ctcagtcgtt cgacttcgag acccagggcc cgttctccat
    15181 ccgggtccgc gctcgggacg cggggaacct cgactacgag gaggtcttct cgatctccgt
    15241 cacgaacgtc aacgacgctc cggtcctcgg ggcggcgacg tacgcgctcg acgagaacag
    15301 cgccaacgtg acggcggtcg ggaccccctc tgctacggac gaggactcgc ccgctcagaa
    15361 cctgacctac tcgatcaccg gcgggaacac gctcggcgcc ttccagatca actcgaacac
    15421 gggggccatc acggtcgccg acgccaccga cctcgacttc gagacgaacc ccacgttcgc
    15481 cctcacggtc caggtcgccg acgacggcac gcccgcgagg gacgactccg agacgatcac
    15541 ggtcaacctc aacaacctga acgagaaacc ggtcgtgaac gcggcgacgc tctctctgcc
    15601 ggagaactcc gcgaacggga cgtccgtcgg caccgtcacc tcgagcgacc cggatgcggg
    15661 ccagaccgtg acttacgcca tcacgggggg caacaccggt aacgccttcg ccatcaactc
    15721 gaacaccggt gagatcaccg tcgccgacgc gaacgacgtc gacttcgagt cgaaccaccc
    15781 gttctcgctg accgtcgagg ggaccgacaa cggggccccg atcctcaccg acaccgagac
    15841 gatcaccgtc aacctcacga acgtcaacga ggccccgacg gacatctcgc tgaacgacac
    15901 ggacgtcgcc gagaacgcgt ccctgaacac ggtcgtcggc acgatctccg gcagcgaccc
    15961 cgacgccggc gactccggca ctctcgtctt cagcctggct tccggcgcag gcgacacgga
    16021 caacgcacgc ttcaacgtgg gtccgtccaa ctcactccgg acctccgagg tcttcgacaa
    16081 ggagacgcag gggccgttct cggtcaggct gcgcgccacc gacggcggaa ccctccagta
    16141 cgaggaggcc ttccagatca gcgtcgtcga cgtcaacgag gctccggacg tcaacgcggc
    16201 cacgttcgcc atcgacgaga acagcgccaa cgggacggcc gtcggaacgg ccacggcgac
    16261 cgacgtcgac gcagggcaga ccctgacgtg ggcgatcacc gggggcaaca ccgggggcgc
    16321 gttccagatc aacccgaaca ccggggcgat caccgtcgcc gacgtcacgg acgtcgaccg
    16381 cgagacgaac cacccgttct cgctgacggt ccaggcaacg gacaacggat cgcctgcgct
    16441 gagcgacacc gacacggtca cggtcaacct caacgacctg aacgacaacc cggtcgtgaa
    16501 cgcggcgacg ctcgcggtcg cggagaacag tgccaacggg acctccgtgg gaacggcgac
    16561 ggcgaccgac caggacaacc ccgcccagac gctcacgtgg gcgatcacgg gcgggaacgc
    16621 cagcggcgcc ttcgctatcg actccggcac cggcgccatc accgtcgcgg acaccggcga
    16681 cctcgacttc gagacgacgc cggcgttcaa cctgacggtc gaggcaaccg acaacggcac
    16741 gccgaaccgg acgggctcga acgcggtcac ggtcaacctg acgaacgtga acgagaagcc
    16801 ggcgatcacg gctcccacga ccatctcggc ccagtacggc gttgcgaagg ccctctcggg
    16861 catctccgtg agcgacccgg actcgggcaa cgccgacatc tcgatgacgc tggccgttct
    16921 cgacgggacg ctcgacctcg acgactccgt cgccaacggc ctggctgccg tcgacatcac
    16981 ggggaacggc acggacgccc tcgttctgac cggcaagcag tcggagatca acaacacgct
    17041 cggcgcggcc accggcctca cctacacgag cgacgccggc ttcctcggcc tgtccgacac
    17101 ccttgcgatc gcgacgaacg acggcgggaa cacgggttcc ggcgccgccc aggtcgacgc
    17161 ggcgaacgtc acgatccagt tcaacacgcc gccggtcgct accgaccaga cggtctcgac
    17221 gaacgaggac accgagaaga cggtcacgct cgccggcacg gacgccgacg ccgacccgct
    17281 gagcttcaag atcacgagtc ttccggcggc cggaaagctg cacgagggca cgagctcggc
    17341 cggcaccgag atcaccgccg gcgcgctgcc gtacacgctg cccgccgaca aggtcaccta
    17401 cgtcccgcct ctgaaccaga acgggctctc gctcgagacg ttcgcattca aggcgaacga
    17461 tggcagcacg gactccgcag caggagtcgt cacggtggac gtcaccgccg tcgacgacat
    17521 ctccgtggcg gagaacgacg cgttctcggt cgacgaggac tcgggggcga cggcgctgct
    17581 ggtccggacc aacgacaccg acgtggacgg cgccgtcgag ctgatcgctt cggtgaccca
    17641 gcccgcgaac gggaccgtca ccaagcacct gggcgacacc cgggtccagt acacgccgaa
    17701 cgcgcattac tgcaacaacc cgccggcgac tcccgtc
//
LOCUS       DASQDO010000479         1710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_907323, whole genome shotgun sequence.
ACCESSION   DASQDO010000479 DASQDO010000000
VERSION     DASQDO010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907323"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1315
                     /locus_tag="VEV43_13205"
     CDS             161..1315
                     /locus_tag="VEV43_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYP24523.1"
                     /translation="MRKVGLNLLWMVPGVVGGSETYTTRLLSGLLEQPSDDLHYTLFT
                     LPQFATAHPEFAADFDLAFAPVTGQWKSFRVAGENSWLAAQAKFKKIDLLHHAGGTMP
                     LVHSARPVLTIHDLQYLFYPEYFTRTKLTWLRQMVPRSAEAARVILVPSEFTRRTVIE
                     RLNIDPSVVIVVPHGISPREPRGPVPDLRERYDLPGPIFLYPAASYPHKNHLLLLEAF
                     KKLLSVHPTALLVLTGARAYLESLTAKQMEARIDEEVRTLGIERSIRRLGFVPSEDLD
                     ALYQEAVATVFPSKFEGFGAPVLEAMSRACPVIAANATALPEVVSDNGLLVSPDNAEE
                     WAETMARLIEDEELRSRLAKLGPERAREFAWARSASILEDAYRHALGTTL"
     gene            1312..>1710
                     /locus_tag="VEV43_13210"
     CDS             1312..>1710
                     /locus_tag="VEV43_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HYP24524.1"
                     /translation="MKLVVLAPHFAPDSAPTGAVVTRIVEQLAARGHRLHVVTALPWY
                     RDHRVVEGFEGRLVRHEDTPWGRITRVYPFPAEDKRDLGARFAGFAGFTALSAVAGAR
                     AGSFDGVLALSPPPTLGVVGWALGRARRAPF"
BASE COUNT          235 a          622 c          603 g          250 t
ORIGIN      
        1 cccgcgcgac cccgcgtcga tcgccgcggg gatcgaacgg gcgctgacgg acgaggcgct
       61 gagaaacgag ctcgcggcgg ccggccacga gcgagctcgg cgctacacgt gggagcggac
      121 gggccggttg ctcgtcgacg cgtaccggga ggtctcgtag gtgcgaaagg tcgggttgaa
      181 cctgttgtgg atggtgccgg gcgtcgtcgg cgggtccgag acgtacacga cgcggctcct
      241 cagcgggctg ctggagcagc cgtccgacga cctccactac acgctgttca cgctcccgca
      301 gttcgcgacc gcgcaccccg agttcgcggc cgacttcgac ctcgcgttcg cgccggtgac
      361 gggacagtgg aagtcgttcc gcgtcgcagg cgagaactcg tggctcgcgg cgcaggcgaa
      421 gttcaagaag atcgacctgc tccaccacgc gggcgggacg atgccgctgg tgcacagcgc
      481 gcggccggtg ctcacgatcc acgacctcca gtacctgttc tatccggagt acttcacgcg
      541 gacgaagctc acgtggctgc gccagatggt cccgcgctcg gccgaagccg cgcgcgtgat
      601 cctcgtgccg agcgagttca cgcggcggac ggtcatcgag cgcctgaaca tcgaccccag
      661 cgtcgtgatc gtcgtgccgc acgggatctc gccgcgcgag ccgcgggggc cggtccccga
      721 cctgcgcgag cgctacgacc tgcccggccc gatcttcctg tacccggccg cttcgtaccc
      781 gcacaagaac cacctgctgc tgctcgaggc gttcaagaag ctcctgtcgg tgcacccgac
      841 cgcgctgctc gtgctgaccg gcgcgcgcgc ctatctcgag tcgctcaccg cgaagcagat
      901 ggaagcgcgc atcgacgaag aggtccggac gctcggcatc gagcgcagca tccggcgcct
      961 cggcttcgtc ccgtccgaag acctcgacgc gctctaccag gaggccgtcg cgaccgtgtt
     1021 cccgtcgaag ttcgagggct tcggggcgcc ggtgctcgag gcgatgagcc gcgcctgccc
     1081 ggtcatcgcg gcgaacgcga ccgcgctccc cgaggtcgtg agcgacaacg gcctcctggt
     1141 ctcgccggac aacgccgagg agtgggccga gacgatggcg cggctgatcg aggacgaaga
     1201 gctccggtct cggctggcga agctcggccc cgagcgggcg cgtgagttcg cctgggcccg
     1261 ctcggcgtcg atcctcgaag acgcgtaccg gcacgcgctg gggacgacgc tgtgaagctc
     1321 gtcgtcctgg cgccgcactt cgcccccgac tcggccccca ccggagcggt cgtcacccgc
     1381 atcgtcgagc agctcgccgc ccgcggccac cggctccacg tcgtcacggc gttgccctgg
     1441 taccgggacc accgggtggt ggagggcttc gagggccggc tcgtgcgcca cgaggacacg
     1501 ccgtggggac ggatcacgag ggtctatccg ttccccgcgg aggacaaacg cgacctgggg
     1561 gcgcgcttcg cgggcttcgc cggcttcacc gcgctgagcg ctgtcgccgg agctcgggcc
     1621 gggtcgttcg acggcgtcct cgcgctctcg ccgcccccca ccctcggcgt cgtcggctgg
     1681 gccctcggcc gggcccgccg cgctccgttc
//
LOCUS       DASQDO010000480         8284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_418507, whole genome shotgun sequence.
ACCESSION   DASQDO010000480 DASQDO010000000
VERSION     DASQDO010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418507"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VEV43_13215"
     CDS             <1..480
                     /locus_tag="VEV43_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24525.1"
                     /translation="VPGTEVLGGTTVAQGQTSTTEGTTTGSAGTGSTTSNDAKAKTKG
                     GSTAGSAATGGSGSEPETVVLGETLTRPEGSSETRAAGRSDSAEGTELLGWVVPLEGA
                     AFGATALFLVAVVGLFFFLLIKRRKRTNYVHSTEFGLARQLGYWTIRTFEGRIFGQI"
     gene            674..2179
                     /locus_tag="VEV43_13220"
     CDS             674..2179
                     /locus_tag="VEV43_13220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYP24526.1"
                     /translation="MEQLLGRAPLSESTSLTQGLVQTAHLDRLELPLGGPAGERSPDA
                     VGRLYRRIAAKLALADAACLVFALMVSYLVRFGFSPLPGEWVALVATAPFVWIGVFHA
                     FHLHSPQHTSSWEQFKNVISASGVGVIAVAMVSFWSHSTFSRAWLGLTWGVALVAELV
                     VRRLFDRHIAKARADGRLAFRTLIVGANNEAGSLAHALKDRHLGFRPLGYVSVETSPV
                     EANGIRVLGHIDDIECVIEDHQAECLFVASSAVSAEDMLRVAQASRRKGVELKVSANV
                     PQMLTSRLNVQPVGDVMSLSLRPVRLTRGQMFTKRALDVVVSAAALLVAAPVLAVVAV
                     AVKLSSPGPVLFKQKRVTKDGHVFSVLKFRTMRTDGDRILEEMGIDPKVPFFKLQDDP
                     RVTRIGNLIRKTSIDELPQLINVIRGEMSLVGPRPLPVDQVLANEHLLSPRHEVKSGV
                     TGWWQIQGRSDVTPEEALRLDLFYIENWSLSLDVYVLLKTIEVLIKRKGAY"
     gene            2230..3867
                     /locus_tag="VEV43_13225"
     CDS             2230..3867
                     /locus_tag="VEV43_13225"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HYP24527.1"
                     /translation="MDQPPAGETFELREYLAVLRTRKWTVLLVTALVVAGALLLSFRQ
                     TPQYRGTSRLLVKALPEDASGEIRPPNLDTEAQLVTSEVVGERVVDELGLSVTPKDLI
                     DGLEVAAVTEVAEVLELTFTSPDPELARDVANAFATEYIEYKKDRTAEALESGERAIT
                     AQIDSVEERLAEVTRELEETNPRADSALASTLETERSSLIARLGVLQQRADDYRARRP
                     IDLDGGQLLEAASLPGSPSSPNHPANGTRALVLGLALGIGIAFLRERLDDRLRGRGDL
                     EASLGLPVLATVPRFPRSGKNAKEIVLVSQPRGSASEAYRSLRTSLHFLSGQSQLKSL
                     LVTSPNEGEGKTVTCINLAVAFAQSGRRVILISADLRRPTLEEYFALGEREGLTNWLL
                     ADEREPWEMIWDPGIDNLRVIPTGSIPPNPAELLSSNRLTHLIEVVEANSDLVLVDSP
                     PVLAVADAAILSTHVDATLLVVDAEQTHRSAAALAAEELHRVGAGLIGSVLNGFDPGA
                     TPYGGKSYYATYESRPPAEESDGSKKQRTRLGFRAEQ"
     gene            3884..5233
                     /locus_tag="VEV43_13230"
     CDS             3884..5233
                     /locus_tag="VEV43_13230"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYP24528.1"
                     /translation="MAVVEAQRRRPLHLPARLAVPARRSPFGWFLFLLLFAVIVPREA
                     AGIDSPSPSWFYLTAFGDFRWIDVIVFGAFGLVAALRIGSDRFFKLHAPKDMGRWSVL
                     MVLAIVLAMVHGSLAGGTELFFDWQGLALGAAFMLVLYQLVRTKPGAVRVAAFALMAV
                     LVGRAAYQLATFFAGTSGVTLQGVRIPVFDGPTLSNTVFVLIAAGCLAVGWKRGPVTT
                     RVLCSAAALLALVLVVLAFRRTYWGEAFVGLVAILLVFNKRRLSALLALAALGVALLP
                     YLPGEFRLRAESANPFAGEENRYTVTNEDHLNDLRDAWDVIKQDPVLGYGLGRPYETE
                     RIENWKHTSWGVHNAVLHVWLRYGALGLVSYLGLHLATLRWLLRLSRRFDPDSFERWW
                     LIAGFSYVTATLVVSLFASPWPYGASQNSMLILGFVGTAFALQNQLRSRERSAPRLG"
     gene            5119..6384
                     /locus_tag="VEV43_13235"
     CDS             5119..6384
                     /locus_tag="VEV43_13235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP24529.1"
                     /translation="MALRGVAEQHADPGLRRDRLRAAEPAAQPGKERASPRVVHYVPK
                     FSRSNWGGAESAIVNLSGSLRRAGYEPEVWTTNSFGDAPREEVEGLAVRRFEGRFFSA
                     REAAMAGLGKAAFSLPMLRALLFERAPDVLHVHTHNRLASALVMAARLRRVPVVVTLH
                     SEFRRHRPRWLYWFPNEAAIRFGDAVISVNRAIADELVRAGIRQEGVVSIPNGVDPAR
                     FAHGDGRRFRAEHGLGDAPVVLTPGRVCDVKNQRVAVDVVPRLAARGVDARWVIVGAP
                     SEPEYFESLRDAVGRPEVSSRILLLPGFPPESPELADAYAAADLVVVPSKHEAFGLVV
                     LEAWCAGKPVVATRVGGLPELVEDGVTGRLVAPGDVSGLADAVAGLLGDPGERGRLGD
                     AGSAKASEFAWGNVGSRTVSVYDRIARSA"
     gene            6390..7865
                     /locus_tag="VEV43_13240"
     CDS             6390..7865
                     /locus_tag="VEV43_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HYP24530.1"
                     /translation="MKRAGSGSLARLTKETLLYLPAIGGPALVGVLGLVVLTRLVSPS
                     VYGRYAVALSVATMAAAVLGDWLTPTLIRFHSEPGDARTGELGQYLVLAWVSSVGATL
                     IVLVSLGFIFPLALTAAAVVLSVALIHQKLGLAVLRAKLLTRAYSMISVAVSLASFAG
                     GIGWYLATRRIEVLLWASAIVLVASLLPAARIAGIVRSDLAGSVDRARIKELSSFGIP
                     IVVTAVGAQALLLADRYLLTTLSGEAAAGLYVPNYAIAERAVGFAFTPLFSALYPLAA
                     RAWAREDRTEAVRYLTLSHRMFVVAGGYVCLVLVLMGDRVAGFMLGDDFVEGSSVIGL
                     VALGNLAWFEGILFHQPLELDKRTRAITMQALTAGVLNIGLNVALIPPFGLMGAAWAT
                     LGSYAAYTVTAYLWSKRSVGVQAEVPWATIWRVGAFGLLAWGATAIVETSGWGDVVFL
                     ASSAVSYIMWLLLTREPVVGELRALMQARSARAASAGRPSE"
     gene            7862..>8284
                     /locus_tag="VEV43_13245"
     CDS             7862..>8284
                     /locus_tag="VEV43_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24531.1"
                     /translation="MTGRLGGTPERMTGRLGGTPERMTIRVAMLADYPIPEAIERSVG
                     LPPSQGFVATPVVNLVDALGRRGDVSVDVVRIAKGIDSDSTHELSRNVRLHLLRVPRV
                     SGMPVAFLPRLAAVLKYVRSLGCDVVHGQGTEAGYAWMA"
BASE COUNT         1125 a         2947 c         2855 g         1357 t
ORIGIN      
        1 gtgcccggca ccgaggtcct aggcgggacc acggtcgctc agggacagac gtcgacgacc
       61 gagggaacga ccaccggctc ggccggcacg ggctcgacga cctcgaatga cgccaaggcg
      121 aagaccaagg gcggcagcac ggccggctcg gccgccaccg gcgggtcagg gtcggagccc
      181 gagaccgtgg tcctcggcga gacgctcacg cgccccgagg gttcctccga gacccgcgcc
      241 gccggtcgct ccgactccgc cgagggcacc gagctcctcg gatgggtcgt cccgctcgag
      301 ggagctgcat tcggagcgac ggcgctgttc ctcgtggccg tcgtcgggct gttcttcttc
      361 ctcttgatca agcgcagaaa gcgtacgaat tatgttcatt cgactgaatt tggtctagcc
      421 cgacaattgg gttattggac aatccgtacg tttgaaggtc gaatttttgg gcagatttaa
      481 ctgccaaatt ttcggacacc ccgcaggggc accccgctct cgcgtcgtac ccctcctccc
      541 agacacctat ccaagcaacc cgccagcccc cccggatagg tccttggaca gacgtaggcc
      601 atcgcgggcc tgatcccgtc tcgatcgcta ggagccatgc gtgtccgcag tcaccgcttt
      661 cgaagctgga gtcgtggaac agctgctcgg ccgagccccc cttagcgagt cgacgtccct
      721 cacccagggc ctcgtccaga cggcccacct cgatcggctc gagctcccgc tcggcgggcc
      781 cgcgggggag cggtcccccg acgccgtggg ccggctctac cgccgcatcg ccgccaagct
      841 cgccctcgcc gacgcggcgt gcctcgtctt cgcgctgatg gtctcgtacc tcgtgcggtt
      901 cgggttctcc ccgcttcccg gcgaatgggt cgcgctggtc gcgacggccc ccttcgtctg
      961 gatcggcgtc ttccacgcct tccacctcca ctccccccag cacacgtcga gctgggagca
     1021 gttcaagaac gtcatcagcg ccagcggcgt gggcgtcatc gccgtcgcca tggtgagctt
     1081 ctggtcgcac tccacgttct cccgtgcctg gctgggcctg acctggggcg tcgcgctcgt
     1141 cgcggagctc gtcgtccggc ggctcttcga ccggcacatc gccaaggccc gggcggacgg
     1201 acggctcgcg ttccggacac tgatcgtcgg cgccaacaac gaggccgggt cgctcgccca
     1261 cgctctcaag gaccgtcacc tcggcttcag gccgctcggg tacgtctcgg tcgagacctc
     1321 gcccgttgag gcgaacggga tccgcgtcct cgggcacatc gacgacatcg agtgcgtcat
     1381 cgaagaccac caggccgagt gcctgttcgt cgcctcgtcc gcggtgagcg ccgaggacat
     1441 gctgcgcgtc gcgcaggcga gccgccgcaa gggcgtcgag ctcaaggtct cggcgaacgt
     1501 gccgcagatg ctgacctcgc ggctgaacgt ccagcccgtc ggcgacgtga tgagcctctc
     1561 gctcaggccc gtccgcctca cccgtggcca gatgttcacg aagagggccc tggacgtcgt
     1621 cgtgtccgca gcggctctcc tcgtggcggc ccccgtcctc gcggtcgtcg ccgtcgccgt
     1681 caagctcagc tcgcccggcc cggtcctgtt caagcagaag cgcgtgacga aggacgggca
     1741 cgtcttctcg gtcctcaagt tccgcacgat gaggacggac ggcgaccgga tcctcgagga
     1801 gatggggatc gaccccaagg tcccgttctt caagctgcag gacgaccccc gggtgactcg
     1861 catcggcaac ctgatccgga agacgagcat cgacgagctg ccccagctca tcaacgtgat
     1921 ccggggagag atgagcctcg tcggcccccg gccccttccc gtggaccagg tcctggccaa
     1981 cgagcacctg ctgtcgccga ggcacgaggt gaagtccggc gtgacgggct ggtggcagat
     2041 ccaggggcgc agcgacgtga cgccggagga ggcgctgcgt ctcgacctct tctacatcga
     2101 gaactggtcg ctctcgctgg acgtttacgt cctgctcaag acgatcgagg tcttgatcaa
     2161 gcgcaagggc gcttactgac acgcaacggc ccgcagcttt ccggcgatac ctggcactag
     2221 gatcgcctga tggatcagcc gccggccggc gagaccttcg agctccgcga gtacctcgcg
     2281 gtcctccgca cgcggaagtg gaccgtgctg ctcgtcaccg cgctcgtggt ggcgggggcc
     2341 ctgctgctgt cgttccgcca gaccccgcag taccggggca cgtcgcggct gctcgtcaag
     2401 gcgcttcccg aagacgcgtc cggagagatc cggcccccga acctcgacac cgaggcgcag
     2461 ctcgtcacgt ccgaggtcgt gggggagcgc gtcgtcgacg agctggggct gtcggtcacc
     2521 ccgaaggacc tgatcgacgg gctcgaggtg gcggcggtga cggaggtcgc ggaggtgctc
     2581 gagctgacgt tcacgtcgcc cgaccccgag ctcgcgcgcg atgtcgcgaa cgccttcgcc
     2641 accgagtaca tcgaatacaa gaaggaccgg accgccgagg ccctcgagag cggcgagcga
     2701 gccatcaccg cgcagatcga ctccgtcgag gaacgcctgg ccgaggtgac ccgcgagctc
     2761 gaggagacga acccccgcgc cgacagcgct ctcgccagca ccctcgagac cgagcggagc
     2821 tcgctgatcg cccggctcgg ggtcctccag cagagggcag acgactaccg ggcccggcgg
     2881 ccgatcgacc tcgacggcgg ccagctgctc gaggccgcga gccttcccgg gtctccgtcc
     2941 tcgcccaacc atccggccaa cggcacccgg gcgctcgtgc tcgggctcgc cctcgggatc
     3001 ggcatcgcct ttctgaggga gaggctcgac gaccgcctcc ggggacgggg ggacctcgag
     3061 gcctccctcg ggctccccgt gctggcgacc gtgccccggt tcccgcgctc cggcaagaac
     3121 gcgaaggaga tcgtgctggt ctcgcagccc cggggctccg cgagcgaggc gtaccgcagc
     3181 ctgcggacga gcctccactt cctctccggc cagagccagc tcaagagcct gctcgtcacg
     3241 agcccgaacg agggcgaggg caagaccgtc acttgcatca acctcgccgt cgccttcgcc
     3301 cagtccggac gcagggtcat cctcatctcc gccgacctga ggcgccccac gctggaggag
     3361 tacttcgcgc tgggcgaacg cgagggcctg acgaactggc tgctcgccga cgagagagag
     3421 ccgtgggaga tgatctggga tcccgggatc gacaacctgc gggtcatccc gaccggctcg
     3481 atcccgccga accccgcgga gctgctttct tcgaaccgcc tgacgcacct gatcgaggtg
     3541 gtcgaggcga actccgacct cgtcctcgtg gactccccgc cggtcctggc ggtcgcggac
     3601 gcggcgatcc tgtccaccca cgtcgacgcg acgctgctgg tcgtcgacgc ggagcagacc
     3661 caccggtcgg ccgcggccct cgccgccgag gagctgcatc gcgtgggggc cggcctcatc
     3721 gggtcggtgc tgaacgggtt cgaccccggg gcgaccccgt acggcgggaa gagctactac
     3781 gccacctacg agagccgtcc gccggccgaa gagagcgacg gttcgaagaa gcaacgcacg
     3841 cgactcggtt ttcgcgcgga acagtgaccg gcccgggtcc tagatggccg tcgtagaggc
     3901 gcagcgacgc cgccccctcc accttccggc ccgcctcgcc gtcccggcgc ggcggtctcc
     3961 gttcggctgg ttcctcttcc tgctcctgtt cgccgtgatc gtccccaggg aggccgcggg
     4021 gatcgactcg ccgtcgccga gctggttcta cctcacggca ttcggcgact tccggtggat
     4081 agacgtgatc gtcttcggcg cgttcggtct cgtcgccgcg ctgcgcatcg gctccgacag
     4141 gttcttcaag ctccatgcac cgaaggacat gggccgctgg tcggtcctga tggtcctcgc
     4201 gatcgtcctc gcgatggtgc acggctcgct ggccggcggg accgagctct tcttcgactg
     4261 gcaggggctg gcgctcggtg cggcgttcat gctcgtcctc tatcagctcg tccgcaccaa
     4321 gccgggcgcg gtgcgcgtgg ctgcgttcgc gttgatggcc gtcctcgtcg gccgcgccgc
     4381 gtaccagctg gcgaccttct tcgcagggac cagcggggtg acgctgcagg gggtccgtat
     4441 cccggtcttc gacggcccca cgctcagcaa cacggtcttc gtgctcatcg ccgcgggctg
     4501 cctcgcggtg ggctggaagc gcgggcccgt cacgacgcgc gtgctgtgca gcgcggcggc
     4561 cctgctggct ctcgtgctcg tcgtcctcgc gttccggcgc acgtactggg gggaggcctt
     4621 cgtcggcctc gtcgccatcc tcttggtgtt caacaagcgg cggctgtcgg ctctgctggc
     4681 gctcgccgca ctgggcgtcg ccctgctccc gtacctgccc ggcgagttcc gtctgcgcgc
     4741 cgagtcggcg aaccccttcg ccggcgagga gaaccggtac acggtgacga acgaagacca
     4801 cctgaacgac ctgcgcgacg cgtgggacgt catcaagcag gacccggtgc tcggctacgg
     4861 cttgggccgt ccctacgaga ccgagcggat cgagaactgg aagcacacgt cctggggggt
     4921 ccacaacgcc gtgctgcacg tctggctcag gtacggggcg ctcgggctcg tcagctacct
     4981 gggcctgcac ctcgccacgc tgcgctggct gctgcggctg tcgcggcgat tcgatcccga
     5041 cagcttcgaa cgatggtggc tcatcgcggg gttcagctac gtcacggcga ccctggtcgt
     5101 cagcctgttc gccagcccgt ggccctacgg ggcgtcgcag aacagcatgc tgatcctggg
     5161 cttcgtcggg accgccttcg cgctgcagaa ccagctgcgc agccgggaaa ggagcgcgcc
     5221 tcgcctcggg tagtccacta cgtcccgaag ttctcgcgtt cgaattgggg gggcgccgaa
     5281 tcggccatcg tgaacctgtc cggcagcctt cgccgtgccg gctacgaacc cgaggtgtgg
     5341 acgacgaact ccttcggcga cgctccccgc gaggaggtcg aagggctcgc cgtgcggcgg
     5401 ttcgagggcc ggttcttctc ggcccgcgag gcggcgatgg cgggcctcgg gaaggcggcg
     5461 ttctctctgc cgatgctccg cgcgctgctg ttcgaacgtg ctcccgacgt gctccacgtg
     5521 cacacgcaca accgtctcgc gagcgcgctg gtgatggcgg cccggctgcg ccgcgtcccc
     5581 gtggtcgtga cgctccacag cgagttccgc cggcaccggc cgcgatggct gtactggttc
     5641 ccgaacgagg ccgcgatccg gttcggcgac gcggtcatca gcgtcaaccg agcgatagca
     5701 gacgagctcg tgcgcgcggg catccggcaa gagggcgtcg tctcgatccc gaacggcgtc
     5761 gacccggcgc gcttcgcgca cggcgacgga cggcgcttcc gcgccgagca cgggctgggc
     5821 gacgcgccgg tcgtcctgac ccccggccgc gtctgcgacg tgaagaacca gcgggtcgcg
     5881 gtcgacgtcg tcccgcgcct cgccgcccgt ggcgtcgacg cccgctgggt gatcgtcggg
     5941 gcgccttccg agcccgagta cttcgaatcc ctgagggatg ccgtcggtcg ccccgaggtc
     6001 tcgtcccgga tcctgctgct cccgggcttc ccgcccgaga gcccggagct cgcggacgcg
     6061 tacgcggcgg ccgacctcgt cgtcgtgccc tccaagcacg aggcgttcgg gctggtcgtc
     6121 ctcgaggcgt ggtgcgccgg caagcccgtc gtcgcgacgc gagtgggcgg gctgcccgag
     6181 ctcgtcgagg acggggtgac cggccggctg gtggctccgg gcgacgtctc cggcctcgcc
     6241 gacgcggtcg cggggctcct gggggatccg ggtgagcgcg gccgcctcgg cgacgccggc
     6301 tcggccaagg cctccgagtt cgcgtggggc aacgtcggga gccggaccgt gagcgtctac
     6361 gaccgcatcg cgcgctcggc gtagccggga tgaaacgagc cggatccggt tccctcgcgc
     6421 gtctgacgaa ggagacgctc ctctatctcc ccgccatagg agggcccgcg ctggtaggcg
     6481 tcctcggcct cgtcgtcctc acgcggctcg tctctccctc cgtctacggg cgctacgcgg
     6541 tggcgctgtc ggtcgcgacg atggcggccg cggtcctggg ggactggctc accccgacgc
     6601 tcatccgctt ccactccgag ccgggggacg cgcggaccgg agagctcggg cagtacctcg
     6661 tgctcgcgtg ggtgtcgagc gtaggcgcca ccctgatcgt gctggtctcc ctcggcttca
     6721 tcttcccgct cgcgctcacg gctgcggcag tggtgctgtc ggtcgcgctg atccaccaga
     6781 agctcgggct cgcggtgctg agggcgaagc tcctcacgcg cgcgtactcg atgatctccg
     6841 tcgcggtctc gctcgcgtcc ttcgccggcg ggatcggctg gtacctcgcg acccggcgga
     6901 tcgaggtcct gctgtgggcg tcggcgatcg tcctcgtggc gagcctgctc cccgcggcgc
     6961 gcatcgcagg catcgtgcga agcgacctcg cggggtcggt ggaccgggcg cggatcaagg
     7021 agctgtcctc gttcggcatc ccgatagtgg tgaccgcggt gggggcccag gctctgctgc
     7081 tcgcggaccg gtacctgctg acgactctgt ccggcgaggc ggccgccggc ctctacgtcc
     7141 cgaactacgc gatcgcggag cgcgcggtcg ggttcgcgtt cacgccgctg ttcagcgcgc
     7201 tctatccgct ggccgcgcgc gcctgggcgc gagaggacag gacggaggcc gttcgatacc
     7261 tgaccctgtc ccacaggatg ttcgtcgtcg ccggaggcta cgtatgcctc gtgctcgtgc
     7321 tgatggggga cagggtcgcg ggcttcatgc tcggcgacga cttcgtggag gggagctcgg
     7381 tcatcgggct cgtggcgttg ggcaacctcg cctggttcga gggcatcctg ttccaccagc
     7441 ccctcgagct cgacaagagg acccgcgcga tcacgatgca ggcgctgact gcgggcgtgc
     7501 tgaacatcgg gctcaacgtg gcgctgatcc cacccttcgg gctcatgggc gccgcctggg
     7561 ccaccctcgg ctcgtacgcg gcgtacacgg tcaccgcgta cctgtggtcg aagaggtccg
     7621 tcggggtcca ggcggaggtc ccgtgggcca ccatctggcg cgtgggtgcg ttcgggctgc
     7681 tcgcatgggg cgcgacggcg atcgtcgaga cgtcgggctg gggagacgtc gtgttcttgg
     7741 cgtcgtccgc cgtttcgtac atcatgtggc tcctgttgac acgggagccg gtggtcggag
     7801 agctgagggc gctgatgcag gcgaggtccg cgcgggccgc ctcggcggga cgcccgagcg
     7861 aatgacgggc cgcctcggcg ggacgccgga acgaatgacg ggccgcctcg gcgggacgcc
     7921 ggaacgaatg acgatccgcg tcgcgatgct cgccgactat ccgattcccg aggcgatcga
     7981 acggtccgtc gggctccctc cctcacaggg gttcgtcgcg acccccgtcg tgaacctcgt
     8041 cgacgccctc ggccgccgcg gcgacgtctc ggtcgacgtc gtccggatcg cgaaggggat
     8101 cgactccgac tcgacgcacg agctgtcgcg caacgtacgt ctccacctgc tgcgcgtgcc
     8161 cagggtcagc ggcatgcccg tcgcgttcct accgcgcctc gccgccgtcc tcaagtacgt
     8221 gcgctcgctc gggtgcgacg tcgtgcacgg ccaggggacc gaggccgggt atgcgtggat
     8281 ggcg
//
LOCUS       DASQDO010000481         4932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1091702, whole genome shotgun sequence.
ACCESSION   DASQDO010000481 DASQDO010000000
VERSION     DASQDO010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091702"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VEV43_13250"
     CDS             <1..184
                     /locus_tag="VEV43_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24532.1"
                     /translation="WDEFRRDHVTGTVGEVTDRLGRLSDLGVTEVVVTAGALPFQVAD
                     PEDVELLGAEIAPALK"
     gene            239..385
                     /gene="tatA"
                     /locus_tag="VEV43_13255"
                     /pseudo
     CDS             239..385
                     /gene="tatA"
                     /locus_tag="VEV43_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(527..1579)
                     /locus_tag="VEV43_13260"
     CDS             complement(527..1579)
                     /locus_tag="VEV43_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYP24533.1"
                     /translation="MRAVVFAGPGRVAVEDVPIPRVAEADDVVVRVERTAICGSDLHL
                     LHGKTPGMNPGSVIGHEFTGRVREAGAAVTSVAEGDLLLGSFLIACGECEPCRRARFN
                     FCERRRALGLGSLTGDLDGAQAEYVRVPVADLNVRRLPADLDDAGQERVLFAGDILAT
                     GFYAAALADLVPDEVVAVVGAGPVGLFCALAARSLGARTLVVDTDPARAAFATEQYGL
                     DAVSLAPDENAAERIVEVTGGLADVVVEAVGAPAAFKTALRCARDGGRVIVVGVYGAE
                     RYDLPMGMVWGRGLQVRFAGMANVQAHWDATLEAVARDAFDPSKVITHRLALDEAEKG
                     YELFAAREAMKVVMTP"
     gene            1659..1913
                     /locus_tag="VEV43_13265"
     CDS             1659..1913
                     /locus_tag="VEV43_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010837304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HYP24534.1"
                     /translation="MVTIYSTTWCGHCRRLKRQLDEAGIGYREIDIDEQEQYADRIVE
                     ATGGYRTVPTLQIGDRLLVNPTVPEVREALGAAAQTYSLV"
     gene            1923..3080
                     /gene="moeB"
                     /locus_tag="VEV43_13270"
     CDS             1923..3080
                     /gene="moeB"
                     /locus_tag="VEV43_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2841..2843,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HYP24535.1"
                     /translation="MSNFRDLDRRARDEVQEVTPEQVKERLAAGERVFLLDVRENEEV
                     EQGMVPGARHLGRGHFESKAEDVVPDKDAEVVVYCASGVRSVFAARTLAELGYTNAVS
                     MKGGFVRWRDLGYDFTIPRTLDAGQRDRYSRHLILPEVGERGQQKLLESKVLCIGAGG
                     LGSPALLYLAAAGVGTIGIVDSDVVETSNLQRQVIHDTEKVGELKAESAKETIARLNP
                     DVQVNVHAFRIDESNARELISQYDVVVDGCDNFDTRYVVNDTAVDLRVPVIHGSIFRF
                     EGMASTFVPFEGPCYRCLYPEAPPPELAPTUSEAGVLGVLPGIVGSLQCNEALKLLVG
                     YGQPLVGRLLTFDAQSTTFAEVKVRRDPSCPACGDNRVSASKTLEAGTVAP"
     gene            3136..4668
                     /locus_tag="VEV43_13275"
     CDS             3136..4668
                     /locus_tag="VEV43_13275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HYP24536.1"
                     /translation="MKRVLPLLLAVIACASVAPAAGARGKTLPVRDLGGDATVIAVID
                     SAFSPYHWDFHASHMPQAKDSNPANDLPLSTAPDAWLDGFPSPSAFDSYNRLDLTLEP
                     DDKEAQLAALDSADSKKWAEVKPSSREEVNYYWMPGTKVVGALDFGGNQVHGDSGEHG
                     MGTTSVSVGNIHGTCPECLLVFLSYSNPESGEAAIEWALDQPWIDAISNSYGFSTAMR
                     DRIYSGSNTQMQRRATDRGQTIFFSAGNGQANDFSVPNTTTFSSQEGPDWIVTVGAVA
                     PGTHASYSGHGKPADIAGIGSSYPASYGATTVSGRGQGGFGGTSNATPTIAGTYGRAL
                     YMARLALPGPSRSQKGGVIARGGRFECAAVRSDCELGDSKLTATELRTRLLHGAVHSE
                     AGTTVSSFQIGNVPPVGEEEFLSEGHGSYYGRETGKDRDWLKEFDRIMAPLEGRAKTL
                     KRPEGEREWFVVDSYCRQHLWGFWRGGYFVRDKTKLPGDDPDYPVRSSWERSCEHLAP
                     PG"
     gene            4669..4926
                     /locus_tag="VEV43_13280"
     CDS             4669..4926
                     /locus_tag="VEV43_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYP24537.1"
                     /translation="MPDHLKVAVLSDTHTVRATRPVPAGAWPYLESADHILHAGDVCD
                     PTLLVELRSLAPLTVVLGNCDGPDVRDWGATDEVEVELCGV"
BASE COUNT          800 a         1733 c         1709 g          690 t
ORIGIN      
        1 ctgggacgag ttccgaaggg accacgtcac cgggaccgtg ggggaggtga cggaccggct
       61 cggacggctg tcggacctcg gcgtgaccga ggtcgtcgtg accgccggtg ctctcccgtt
      121 ccaggtcgcg gaccccgagg acgtggagct cctaggtgcc gagatcgccc cggcgctgaa
      181 gtgaacggac gcggcggccg acggtaaggg cagccacgaa gaaacggagg tcgtctacgt
      241 gaacctggga cccggcgagc tgatcccgat cctgctgatc ctgctgctgc tgttcggcgc
      301 gacgcggctg ccgaagctcg cccgctcgat gggccaggcc ggcaaggagt tcaaggaggg
      361 cctgaaggag ggctacaagg aagagcccgt cgagggcccg tgtccgttct gtggcgtcaa
      421 ggtcgaggcc gactcgaagt tctgccccgg ctgcggcaag gccgcggccg agatcacggc
      481 cgagaagacc aagcaggccc aggagcagaa gagcgcctag ctccccttac ggcgtcatca
      541 ccaccttcat cgcctcacgc gcggcgaaca gttcgtagcc cttctcggct tcgtccagcg
      601 cgagcctgtg cgtgatgacc ttcgacgggt cgaacgcgtc gcgggccacg gcctcgagcg
      661 tggcatccca gtgcgcctgc acgttcgcca tccccgcgaa gcgcacctgt aggccccgcc
      721 cccagaccat ccccatcgga aggtcgtagc gctcggcgcc gtagaccccg acgacgatca
      781 cgcggccccc gtcacgagcg cagcgcagcg cggtcttgaa cgccgccggc gctcccaccg
      841 cctcgacgac gacgtccgcc agccctccgg tcacttcgac gatcctctcg gcggcgttct
      901 cgtcgggcgc gagcgagacc gcgtccagcc cgtattgctc agtcgcgaac gccgcccggg
      961 ccgggtcggt gtcgacgacc agggtgcgtg caccgaggga ccgcgccgcg agcgcgcaga
     1021 acagcccgac cggaccggct ccgacgaccg cgacgacctc gtcgggaacg aggtccgcga
     1081 gcgcggctgc gtagaagccc gtggcgagga tgtcgcccgc gaacaggacc cgctcctggc
     1141 cggcgtcgtc gaggtccgcc gggagccgcc tcacgttcag gtcggcgacc ggcacccgca
     1201 cgtactccgc ctgcgcgccg tcgaggtcac cggtcagcga gccgagcccg agcgccctcc
     1261 tccgctcgca gaagttgaag cgcgcccggc ggcacggctc gcactcgccg cacgcgatca
     1321 gaaaggagcc gagcaggagg tctccctcgg ccacggacgt caccgcggcc cccgcttcgc
     1381 ggacccggcc ggtgaactcg tgccctatga ccgagcccgg gttcatgccg ggggtcttgc
     1441 cgtggaggag gtggaggtcc gagccgcaga tcgccgtccg ctcgacccgc acgacgacgt
     1501 cgtcggcttc ggcgactcgg gggatcggga cgtcttcgac ggcgacccgg cccggcccgg
     1561 cgaacacgac cgccctcacg gggccgtcac tccggaatcc gggttcatcg cggaacgtta
     1621 ctggactcag acgccgccag agggggaaga cctacgacat ggtcacgatc tactccacga
     1681 cgtggtgcgg ccactgccgc cgtctcaagc gccagctcga cgaggccggc atcggctacc
     1741 gcgagatcga catcgacgag caggagcagt acgcggaccg gatcgtcgag gcgaccggcg
     1801 ggtaccggac ggtcccgacg ctccagatag gggaccggct gctggtcaac ccgaccgtgc
     1861 ccgaggtgcg ggaggccctg ggggccgcgg cccagacata ctcactagtg taaggtggat
     1921 agatgtctaa cttcagggac ctcgaccgcc gggcccgcga cgaggtgcag gaggtcacgc
     1981 ccgaacaggt gaaggagcgg ctcgcggccg gcgagcgcgt cttcctcctc gacgtccgcg
     2041 agaacgaaga ggtcgagcag gggatggtcc cgggcgcccg ccacctcggg cgcggccact
     2101 tcgagagcaa ggccgaagac gtcgtccccg acaaggacgc ggaggtcgtc gtctactgcg
     2161 ccagcggcgt ccgcagcgtg ttcgccgccc ggacgctcgc cgagctcggc tacacgaacg
     2221 ccgtctcgat gaagggcggc ttcgtccgct ggcgcgacct gggctacgac ttcacgatcc
     2281 cgcgcacgct cgacgccggg cagagggacc ggtactcgcg gcacctgatc cttcccgagg
     2341 tgggcgaacg gggccagcag aagctcctcg agagcaaggt cctgtgcatc ggggccgggg
     2401 ggctgggaag cccggcgctg ctctacctgg cggccgccgg ggtcggcacg attgggatcg
     2461 tcgactccga cgtcgtcgag accagcaacc tccagcgcca ggtgatccac gacaccgaga
     2521 aggtcgggga gctcaaggcc gagtcggcca aggagaccat cgcccggctc aaccccgacg
     2581 tccaggtgaa cgtccacgcg ttccgcatag acgagagcaa cgcccgcgag ctgatctcgc
     2641 agtacgacgt ggtcgtcgac ggctgcgaca acttcgacac gcgctacgtc gtcaacgaca
     2701 ccgcagtcga cctgagggtg cccgtcatcc acggctcgat cttccgcttc gagggcatgg
     2761 cgtcgacgtt cgtcccgttc gaggggccgt gctaccgctg cctctatccc gaggcccccc
     2821 cgcccgagct cgcccctacc tgaagcgagg cgggtgtcct gggcgtgctc ccgggaatcg
     2881 tcggatcgct gcagtgcaac gaggccctca agctcctggt cggctacggc cagcccctgg
     2941 tcgggcggct gctgacgttc gacgcgcagt cgacgacgtt cgccgaggtc aaggtgcgcc
     3001 gggacccgtc gtgccccgcc tgtggtgaca accgggtgtc agcgtcgaag actctagagg
     3061 caggaacggt cgcgccctag gagaaccctc ccttcagacc gagaatcggc cgaaatcgga
     3121 cggaggggac ctaccttgaa gcgcgttctg cccttgctcc tcgccgtgat cgcctgcgcg
     3181 tccgtcgcgc ctgcggccgg ggcgcgcggc aagacgctcc cggtccggga cctcggcggc
     3241 gacgcgaccg tgatcgcggt catcgactcg gcgttcagcc cctatcactg ggacttccac
     3301 gcctcccaca tgccgcaggc gaaggactcg aaccccgcca acgacctccc cctgtcgacc
     3361 gctccggacg cgtggctcga cggcttcccg agcccgtcgg cgttcgactc ctacaaccgc
     3421 ctcgacctca cgctcgaacc ggacgacaag gaagcgcagc tcgccgcgct ggactccgcc
     3481 gactcgaaga agtgggcgga ggtcaagccg tcgtcgcgcg aggaggtcaa ctactactgg
     3541 atgccgggga cgaaggtcgt cggcgctctc gacttcggcg ggaaccaggt ccacggagac
     3601 tcgggtgagc acgggatggg aaccaccagc gtctccgtcg ggaacatcca cgggacgtgc
     3661 ccggagtgcc ttctcgtgtt cctcagctac tcgaacccgg agtccggcga ggccgcgatc
     3721 gagtgggcgc tcgaccagcc gtggatcgac gcgatctcga actcgtacgg cttctcgacc
     3781 gcgatgaggg accggatcta ctcgggctcg aacacgcaga tgcagcgccg cgctaccgac
     3841 cgcggccaga cgatcttctt ctcagccggc aacggacagg cgaacgactt ctcggtcccg
     3901 aacacgacga cgttctcgtc gcaggagggt cccgactgga tcgtcacggt cggcgcggtc
     3961 gcgcccggga cgcatgcctc ttactccggc cacggcaagc ccgcggacat cgcggggatc
     4021 gggtccagct accccgcttc gtacggagcc acgacggtct ccggcagggg gcagggcggc
     4081 ttcggtggca cctcgaacgc gactccgacg atcgccggca cgtacgggcg cgcgctctac
     4141 atggcgcgcc ttgccctgcc ggggccgtca cgctcccaga agggcggggt aatcgcgcgc
     4201 ggcgggcggt tcgagtgtgc cgcggtgcga agcgactgcg agctcggcga ctcgaagctg
     4261 acggcgacgg agctgcgcac ccgcctgctg cacggcgcgg tccactccga ggccgggacc
     4321 accgtctcgt ccttccagat cggcaacgtc ccgcccgtcg gcgaggagga gttcctcagc
     4381 gaaggccacg gcagctacta cggccgcgag accggcaagg accgcgactg gctgaaggag
     4441 ttcgaccgga tcatggcgcc gctcgagggc cgggcgaaga cgctgaagcg tcccgagggc
     4501 gagcgcgagt ggttcgtcgt ggactcgtac tgccgtcagc acctgtgggg gttctggcga
     4561 ggcggctact tcgtgcggga caagacgaag ctccccggcg acgacccgga ctaccccgtg
     4621 aggtcgtcgt gggagcgctc gtgtgagcac ctcgccccac ctgggtagtt gcccgaccac
     4681 ctgaaggtcg cggtcctctc cgacacgcac acggtccgtg cgacgcgccc tgtgcccgcg
     4741 ggggcgtggc cgtacctgga gagcgcagac cacatcctgc atgccggtga cgtgtgcgac
     4801 ccgacgctgc tcgtcgagct gaggtcgctc gcgccgctga ccgtcgtgct gggcaactgc
     4861 gacgggcccg acgtcaggga ctggggggcg actgacgagg tcgaggtcga gctgtgcggg
     4921 gtctagatcg gg
//
LOCUS       DASQDO010000482         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_112759, whole genome shotgun sequence.
ACCESSION   DASQDO010000482 DASQDO010000000
VERSION     DASQDO010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112759"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VEV43_13285"
     CDS             <1..819
                     /locus_tag="VEV43_13285"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYP24538.1"
                     /translation="PAPDMGTNAQTMAWMMDEYGKKQGYTPGIVTGKPVELGGSPGRE
                     EATGRGVVICARESAKKMGLNFHGARVAIQGFGNVGYWTAAIAAEAGANVVAVSDVGG
                     GTHNPEGLDLTELGKHAKESVSVANFAGGTEVSNEDLLELDCDILIPAAIHGVINTKN
                     ADRIKAKLVVEAANGPTTPAADEVLHDRGVVVAPDILANSGGVTVSYFEWVQNIQQFR
                     WEVDDVNFQLKKRMSKATTHVFTRAAGDGTSLRDAAFDIAVERVANADKLRGFV"
     gene            1023..1217
                     /locus_tag="VEV43_13290"
     CDS             1023..1217
                     /locus_tag="VEV43_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24539.1"
                     /translation="MSDRSCAWMSGPKEASLSVPAVKWWLAISRDVLAKDCRRMTSRA
                     RPSVTCHRTPTFVSPLQKDS"
     gene            1362..1571
                     /locus_tag="VEV43_13295"
     CDS             1362..1571
                     /locus_tag="VEV43_13295"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HYP24540.1"
                     /translation="MSVEKSIDLTATGATIEDAVAGAVHRASLTLKGLTSFEVERIDG
                     LIESGEVVYRVLVRVTFQIKEQVHE"
     gene            1577..>2217
                     /locus_tag="VEV43_13300"
     CDS             1577..>2217
                     /locus_tag="VEV43_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP24541.1"
                     /translation="MEHRRREPAPGVFRLVLPLPFPGLNAVNAFLLAGDGGATLVDCG
                     IRNPQPDGDHGWDEIKAALAAAESSAGAIERLVVTHTHIDHYGMAARFLEETGCELWM
                     HREAHDEIELYRDPEGAKENLRELLSDHGASPAELDELTQFEDWRPFVAGVVEATHSC
                     DGGETFTAGGREWAVVYTPGHARSHVCLWNARDALLISGDHLLPTITPHIDFER"
BASE COUNT          401 a          725 c          763 g          328 t
ORIGIN      
        1 ccggcccccg acatgggcac gaacgcgcag acgatggcct ggatgatgga cgagtacggc
       61 aagaagcagg gctacacgcc cggcatcgtg acgggcaagc ccgtcgagct cggaggctcg
      121 ccggggcgcg aggaggcgac gggccgcggg gtcgtcatct gcgcgcgcga gtccgccaag
      181 aagatggggc tgaacttcca cggagcgcgc gtagcgatac agggcttcgg gaacgtcggc
      241 tactggaccg cggctatcgc ggcggaggcc ggggcgaacg tggtcgccgt ctcggacgtg
      301 gggggcggca cgcacaaccc cgagggcctc gacctcaccg agctgggcaa gcacgccaaa
      361 gagtcggtgt cggtggccaa cttcgccggg gggaccgagg tgagcaacga ggacctcctc
      421 gagctcgact gcgacatcct gatccctgcc gccatccacg gcgtgatcaa cacgaagaac
      481 gccgaccgca tcaaggcgaa gctcgtcgtc gaggcggcca acggcccgac tacgccggcc
      541 gccgacgagg tgctccacga ccgcggtgtc gtcgtcgctc ccgacatcct ggcgaactcc
      601 ggtggggtca ccgtctcgta cttcgaatgg gtccagaaca tccagcagtt ccgctgggag
      661 gtcgacgacg tcaacttcca gctgaagaag cggatgagca aggcgacgac ccacgttttc
      721 acccgcgccg ccggcgacgg aacgtcgttg cgtgacgccg cattcgacat cgcggtcgag
      781 cgagttgcga atgcggacaa gctgcgcggc ttcgtctgag aggtgtcctg tttaggcccc
      841 cgcgcacccg ggaatttatc cggtgaaatt cgggcattcc tgacgttagg gctgctcaga
      901 gggggttttt ctgaccgcat agggcaggag agggccaccg gatgacgaac ctcgttcatc
      961 aagcgaatgc agtggacccc cacgcaacat ccggtaccgc cgagaccgaa aggtaaggag
     1021 cgatgagcga cagatcttgc gcctggatgt cgggcccgaa ggaggcgtcg ctcagcgttc
     1081 cggccgtgaa gtggtggctg gcgatctccc gggacgtact tgcaaaggac tgccgcagga
     1141 tgacctctcg ggcccgcccg tcggtcacgt gccaccggac tccgacgttc gtatcaccgc
     1201 tccagaagga ctcgtagctc gtagctccgt acgaccgcac caccctccca aggcaacacc
     1261 ctgcacctcc tctggcagga tgaacgaagc tcccgaactg gccgggagct tcgttctttt
     1321 tgtgcgggag gattacgacc acgcacacga gaggagggcg gatgtcagtc gagaagtcga
     1381 tcgacctcac cgcgaccgga gccacgatcg aagacgcagt cgccggcgcg gtgcaccgcg
     1441 ctagcctgac gctgaaggga ctcacctcgt tcgaggtcga gcgtatcgac ggtctcatcg
     1501 agagcggtga ggtcgtctac cgcgtgctcg tgcgcgtgac ctttcagatc aaagagcagg
     1561 tacacgagta gcccgcgtgg agcatcggcg gcgcgagccc gcgccgggcg tcttccgtct
     1621 cgtcctgccc ctgccgttcc cggggctgaa cgcggtcaac gcgttcctgc tcgccggtga
     1681 cggcggcgcg acgctcgtcg actgcgggat aaggaaccca cagcccgacg gcgaccacgg
     1741 ttgggacgag atcaaggccg cgctcgcggc cgcggagtcg tccgccggcg cgatcgagcg
     1801 cctcgtggtc acgcacacgc acatcgacca ctacgggatg gccgcgcgct tcctcgagga
     1861 gacgggatgc gagctctgga tgcaccgcga ggcgcacgac gagatcgagc tgtaccgcga
     1921 cccggaggga gccaaggaga acctgcgcga gctgctctcg gaccacggcg cgagcccggc
     1981 cgagctcgac gagctgacgc agttcgagga ctggcggccg ttcgtcgccg gcgtggtcga
     2041 ggcgacgcac tcctgcgacg ggggcgagac gttcaccgcc gggggccgcg agtgggcggt
     2101 cgtctacacc ccgggccatg cccggtcgca cgtgtgcctg tggaacgccc gggacgctct
     2161 gctgatctcg ggcgaccatc tgctgccgac gatcacgccg cacatcgact tcgagcg
//
LOCUS       DASQDO010000483         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_419046, whole genome shotgun sequence.
ACCESSION   DASQDO010000483 DASQDO010000000
VERSION     DASQDO010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419046"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VEV43_13305"
     CDS             complement(<1..995)
                     /locus_tag="VEV43_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP24542.1"
                     /translation="MVPVPSDELERKIADYDREVQDLRGQVKLLEDELALTRRKLEAA
                     PRRIHELERRLSESITELTQSRETGERMAEKLREARDQLVALKEEVEKLSQPPSGYGV
                     FLQAYEDNQVDVFTNGRKLRVNVAPEVDLEQLERGREVMLNEAMNVIGVCDFEIQGEV
                     VILKERLDEGRALVLGRTDEERVVELADSLKDVHIRAGDSLLLEPKSGHVLEKLPKPE
                     VEELILEEVPDVSYEDIGGLDGQIEEIRDAVELPYLHGDLFREHQLEPPKGILLYGPP
                     GCGKTLVAKAVANSLAKKVAAKLGRDDRRSYFLNIKGPELLNKYVGETERQIRMI"
     gene            complement(1081..1899)
                     /locus_tag="VEV43_13310"
     CDS             complement(1081..1899)
                     /locus_tag="VEV43_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HYP24543.1"
                     /translation="MNLEAGEKVLLIDSRDRRYLVTLATGKQFHSHAGVVEHDALIGK
                     PEGSTVRSSGGAKLVAFRPTLAEFVLKMKRGAQVVYPKDVALILVYADVFPGATVLEA
                     GSGSASLTLALARAVGESGRVISYELRDDHHAQAVANVTAWYEGLGAKPENLEFRVGD
                     VFSDVEEDVVADRMVLDLPEPWHAIGTTTERLKPGGILCCYLPTVPQVSQLVETMRRG
                     GFGRIETMESLVRTWNVDGQSVRPDHRMVAHTGFVVTARKLAPPSNSSEPQNTP"
     gene            complement(1896..2873)
                     /locus_tag="VEV43_13315"
     CDS             complement(1896..2873)
                     /locus_tag="VEV43_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HYP24544.1"
                     /translation="MKCKVCKGKAVVDVRRHNAGFCRDHFVDHVRNQVAKTIKEFSMF
                     SPEDRLRIAVSGGKDSLALWDLLLDLGYDATGFYLDLGIGGYSTRSKDASIAFAEGRD
                     AKLIVRSLGEEHGYTVPELAKLTGRVPCSGCGLNKRYEFNRAALEEGFDVLVTGHNLD
                     DEVATLFGNVLHWNTDQLGRQAPVLEERILGDAPEPVLVRKCKPLVRIAERETAAYAL
                     LRGIDYVIEECPMVEGNTQHRYKEAISLLEEASPGTKHQMYFGFLKKAASKFAESDED
                     ASLVRCLSCGAPTLDWGQDEPQCSFCKTKALALKRKETGERPRPRRSRR"
     gene            complement(2870..3109)
                     /locus_tag="VEV43_13320"
     CDS             complement(2870..3109)
                     /locus_tag="VEV43_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYP24545.1"
                     /translation="MKVKLRNPDRVVDVSGPKNVTALLQHLGIVPEAVLVIRDATLLT
                     RDEAVSEDDEIEVRPVLSGGSGGLGRRSEGRGTGP"
     gene            complement(3134..>3527)
                     /locus_tag="VEV43_13325"
     CDS             complement(3134..>3527)
                     /locus_tag="VEV43_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYP24546.1"
                     /translation="AAENIGFAIPIDKALPIVEQILEDPPTERAWLGVTLGDVTAEAA
                     IEFGLPDEVEGAVVLAVVPDSPADEAGLEVGDVVLAIEGEGVGGADDLVQELRRREVG
                     ETVELDVFTDGDEDTIDVELERRPVTLG"
BASE COUNT          500 a         1237 c         1183 g          607 t
ORIGIN      
        1 aagatcatgc ggatctgccg ctcggtctcg cccacgtact tgttcaggag ctcggggccc
       61 ttgatgttga ggaagtacga gcgacggtcg tcgcgtccga gcttggccgc gaccttcttc
      121 gccagcgagt tcgcgacggc cttcgcgacg agcgtcttgc cgcaccccgg cgggccgtag
      181 agcaggatcc ccttgggcgg ttcgagctgg tgctcgcgga acaggtcgcc gtgcaggtac
      241 gggagctcga cggcgtcgcg gatctcctcg atctgtccgt cgaggccgcc gatgtcctcg
      301 tagctcacgt ccggcacctc ttcgaggatc agctcctcga cctcgggctt cggcagcttc
      361 tcgaggacgt gaccggactt cggctcgagc agcaggctgt cgccggcgcg gatgtggacg
      421 tccttgagcg agtccgcgag ctcgacgacg cgctcctcgt ccgtgcgccc gaggacgagg
      481 gcccggccct cgtcgagacg ctccttcagg atcacgacct cgccctggat ctcgaagtcg
      541 cagacgccga tgacgttcat cgcctcgttg agcatgacct cgcggccgcg ctcgagctgc
      601 tcgaggtcga cctcgggcgc gacgttcacg cgcagcttgc ggccgttcgt gaagacgtcg
      661 acctggttgt cctcgtaggc ctggaggaag acgccgtagc cggacggagg ctggctgagc
      721 ttctcgacct cctccttcag cgcgacgagc tggtcccgcg cctcgcggag cttctcggcc
      781 atgcgctcgc cggtctcgcg cgactgggtg agctcggtga tcgactccga caggcggcgc
      841 tcgagctcgt ggatgcggcg cggagccgct tcgagcttgc ggcgcgtgag cgccagctcg
      901 tcctcgagga gctttacctg gccacgaaga tcctggacct cgcggtcgta gtcagcgatc
      961 ttccgctcga gctcgtcgga cggaaccgga accacctccc tctcccaggg ttccggggcg
     1021 gcggaaagac gccacggccc ccggacctgc caagccccga tgctactccc tgtactaggt
     1081 ctacggcgtg ttttggggct cgcttgaatt actgggaggg gcgagcttcc gggccgtcac
     1141 cacgaagccc gtgtgggcga ccatgcggtg gtccggccgc acggattgcc cgtctacgtt
     1201 ccacgtcctc acgaggctct ccatcgtctc gatgcggccg aagccgcctc gcctcatcgt
     1261 ctcgaccagc tgcgacacct gcgggaccgt ggggaggtag cagcagagga tgccgccggg
     1321 cttcagccgc tccgtcgtcg taccgatcgc gtgccacggc tcggggaggt cgagcaccat
     1381 ccggtccgcg accacgtctt cctcgacgtc ggagaagacg tcgccgacgc ggaactcgag
     1441 gttctcgggc ttggcgccga gtccctcgta ccaggccgtg acgttcgcga ccgcttgagc
     1501 gtgatggtcg tcgcgcagct cgtacgagat cacgcgcccg ctctccccga cggcgcgcgc
     1561 gagcgcgaga gtcagcgacg cggagccgct gccggcttcg aggacggtgg cgccggggaa
     1621 cacgtccgcg tagacgagga tcagcgcgac gtccttcggg tacacgacct gcgcgccgcg
     1681 cttcatcttg aggacgaact ccgcgagcgt cgggcggaac gcgacgagct tcgctccccc
     1741 gctgctcctg accgtgctgc cctcgggttt gccgatcagc gcgtcgtgct ctacgacgcc
     1801 ggcgtgcgag tggaactgct ttccggtcgc gagcgtgacg aggtagcgcc ggtcgcgcga
     1861 gtcgatcagc aggaccttct cgcccgcttc gagattcatc tgcgcgaacg ccggggccgc
     1921 gggcgttcgc cggtctcctt gcgcttcaaa gcgagggcct tcgtcttgca gaaggagcac
     1981 tgcggctcgt cctgccccca gtcgagcgtc ggcgcaccgc agctgaggca gcggacgagg
     2041 ctcgcgtcct cgtcgctctc cgcgaacttc gacgcggcct tcttcaggaa cccgaagtac
     2101 atctggtgct tggtcccggg cgacgcctcc tcgagaaggg agatcgcctc cttgtagcgg
     2161 tgctgcgtgt tgccctcgac catcgggcat tcctcgatca cgtagtcgat gccgcgcagc
     2221 agcgcgtacg ccgccgtctc gcgctcggcg atccgcacga gcggcttgca cttgcgcacg
     2281 aggaccggtt ccggcgcatc tccgaggatc cgttcttcga ggacgggggc ctggcgaccg
     2341 agctggtcgg tgttccagtg cagaacgttg ccgaacagcg tcgcgacctc gtcgtcgagg
     2401 ttgtggccgg tgacgaggac gtcgaagccc tcttccagcg cagcccggtt gaactcgtaa
     2461 cgcttgttga gaccgcagcc cgagcacggg acgcggccgg tgagcttcgc aagctcgggg
     2521 accgtgtagc cgtgctcctc gccgagcgaa cgcacgatga gcttcgcgtc gcgcccctcg
     2581 gcgaaggcga tcgacgcgtc cttcgaccgg gtcgagtacc cgccgatgcc gaggtcgagg
     2641 tagaagccgg tcgcgtcgta gccgaggtcc aggagcaggt cccacagcgc gagcgagtcc
     2701 ttgcccccgg agaccgcgat ccggagccgg tcctccggcg agaacatcga gaactccttg
     2761 atcgtcttcg cgacctggtt gcggacgtgg tcgacgaagt ggtcgcggca gaacccggcg
     2821 ttgtgccgcc gcacgtcgac gacggccttc cctttacaga ccttgcactt cacgggccag
     2881 tgccacgtcc ctcggagcgc cggccgaggc cgccgctgcc cccggagaga acagggcgga
     2941 cctcgatctc gtcgtcctcg gagaccgcct cgtcgcgcgt cagcagcgtc gcgtcgcgga
     3001 tcacgaggac cgcctcgggg acgatgccga ggtgctgcaa gagcgcggtc acgttcttgg
     3061 gcccgctcac gtcgacgacg cggtccgggt tgcggagctt caccttcatg accccagcgt
     3121 aacggggccc ccgctagccg agggtgaccg gccgcctctc gagctctacg tcgatggtgt
     3181 cctcgtcgcc atcggtgaag acgtccagct cgacggtctc gcccacctcc cggcggcgca
     3241 gctcctggac gaggtcgtcg gcacccccga ccccctcacc ctcgatggcg aggacgacgt
     3301 cacccacctc cagcccggct tcgtcggcgg ggctgtcggg gaccacggcg agcacgacgg
     3361 cgccctccac ttcgtccggc aggccgaact cgatcgctgc ctcggccgtc acgtcgccga
     3421 gcgtcacgcc cagccacgcc cgctccgtcg gcgggtcctc gaggatctgc tcgacgatcg
     3481 ggagcgcctt gtcgatcggg atcgcgaagc cgatgttctc cgcggcc
//
LOCUS       DASQDO010000484         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_785984, whole genome shotgun sequence.
ACCESSION   DASQDO010000484 DASQDO010000000
VERSION     DASQDO010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785984"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2374
                     /locus_tag="VEV43_13330"
     CDS             <1..2374
                     /locus_tag="VEV43_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24547.1"
                     /translation="TPYGEARGYIDLHLHMMAFEFIGGRSRCGRPWHRFGVTHALVDC
                     PDHEPGGHGAVLEAALSGGNPVEGHDTDGWPTFEGWPKPHSLTHEQVYYKWLERSWRG
                     GLRLFVNLLVDNGQLCEIYPYRRNSCDEMENVRLQARRIRELERYVDAQSGGPGEGWF
                     RVVDDPFEAREVINDGKLAVVLGMEVSVPLGCGIVRDLPTCPADEIDDRLQEVYDLGV
                     RQMELVNKFDNAFTGVKGDSDLQGPIVNFGNFLETGSYWRMDTCSEDDGHSHDHGVAH
                     DHTQMNFHDESGAPDEFSGRDAIFGAVLSLFGQTGAAPVYPEGPHCNSLGLSDMGRTA
                     IREMIERGMIFDPDHMSSHARTDAMDLVAQIGEELRAEGSTVTPGVVSSHSWADDTIY
                     RRIYELGGVVTPHGGGSASFVGKWRKHRDWADERFLFGIGYGSDTNGFSNQGQPRSGN
                     ETNPVEYPFEGFGGATVHKQVSGTRAPYDVNRDGVAHYGLYTDWIEDLRVQAGQPIVD
                     DMLLGPEAYLQMWERAIGVAGSSCRTDVADLTPEDFERLRTGMTAEEVLRTLGQPRSR
                     SGNEFAYCLTWDRTGTVRFTSGGEVESWDTGGAVAVVLDDANPSTGQVTDAARFAATV
                     TDGSGAPVAGQPVTFTVGSASAPATSDETGRAVASVVLEGPAGEQGVTVSAGSLTGSA
                     PFTVTREDTLLTLFDAVASGNDPAMARAGLAEDGGMVAGRTISFYVADKVGGRKSATL
                     IGTAVTDVNGMAAIEVPQRYVSSSRRTITATFAGDGDFLPASGTASAYRG"
     gene            complement(2371..2733)
                     /locus_tag="VEV43_13335"
     CDS             complement(2371..2733)
                     /locus_tag="VEV43_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24548.1"
                     /translation="MAITGTHMLLYTSEPDELRAVLRDVFGFSHVDSGDGWLIFALPP
                     AELGIHPAEGPTYDSGTRHQLTFMCDDVNATVAELRERGVEVRGEPEDEGWGITTTLV
                     LPGGVEVMLYEPRHPTAI"
     gene            2756..>3095
                     /locus_tag="VEV43_13340"
     CDS             2756..>3095
                     /locus_tag="VEV43_13340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP24549.1"
                     /translation="MEPRDVIVLGGGPAGYACALRLSDLGLSCVLVEAADLGGTCLHR
                     GWVPTRAMSEVAAIADTLARKAERWGIHAVLEKVDYARLLATRDEIVARNHVSIRHHL
                     ERARVDVVAGY"
BASE COUNT          464 a         1064 c         1124 g          443 t
ORIGIN      
        1 gacgccgtac ggcgaggccc gcggctacat cgacctccac ctgcacatga tggcgttcga
       61 gttcatcggc gggcggtcgc gctgcggccg gccgtggcac cggttcggcg tcacgcacgc
      121 gctcgtcgac tgccccgacc acgagccggg cgggcacggc gcggtgctcg aggcggcatt
      181 gagcggcggc aacccggtcg aggggcacga caccgatgga tggccgacgt tcgagggctg
      241 gccgaagccg cactcgctca cgcacgagca ggtctattac aagtggctgg agcgttcctg
      301 gcgcggcggc ctccggctct tcgtcaacct cctcgtcgac aacgggcagc tctgcgagat
      361 ctatccgtac cgccgcaaca gctgcgacga gatggagaac gtccgtctcc aggcgcgcag
      421 gatccgcgag ctcgagcggt acgtcgacgc gcagagcggg gggccgggcg aaggctggtt
      481 ccgggtcgtc gacgacccct tcgaggcgcg cgaggtgatc aacgacggca agctcgcggt
      541 cgtgctcggc atggaggtgt cggtcccgct cgggtgcggg atcgtgcgcg acctcccgac
      601 gtgcccggcc gacgagatcg acgaccgcct ccaggaggtc tacgacctcg gcgtgcgtca
      661 gatggagctc gtcaacaagt tcgacaacgc gttcacgggc gtcaagggcg actccgacct
      721 ccagggcccg atcgtgaact tcgggaactt cctcgagacc gggtcgtact ggcgcatgga
      781 cacgtgctcg gaagacgacg ggcactcgca cgaccacggc gtcgcccatg accacacgca
      841 gatgaacttc cacgacgagt ccggcgcgcc cgacgagttc tcggggcgcg acgcgatctt
      901 cggcgcggtg ctgtcgctgt tcggccagac gggcgcggcc cccgtgtacc cggagggccc
      961 gcactgcaac tcgctcgggc tgagcgacat ggggcgcacg gcgatccgcg agatgatcga
     1021 gcgggggatg atcttcgacc ccgaccacat gagctcgcac gctcgcaccg acgcgatgga
     1081 cctcgtcgcg cagatcggcg aggagctgcg tgccgagggc tcgaccgtca cgccgggcgt
     1141 cgtgtcgagc cattcgtggg ccgacgacac gatctaccgc cgcatctacg agctgggtgg
     1201 cgtcgtgacg ccgcacggcg gcggctccgc gagcttcgtc ggcaagtggc gcaagcatcg
     1261 cgactgggcc gacgagcggt tcctgttcgg gatcggctac gggtcggaca cgaacggctt
     1321 cagcaaccag ggacagcccc gctccggcaa cgagacgaac ccggtcgagt acccgttcga
     1381 gggcttcggc ggcgcgacgg tccacaagca ggtcagcggc acgcgtgctc cctacgacgt
     1441 caaccgcgac ggcgttgccc actacgggct ctacacggac tggatagagg acctccgcgt
     1501 gcaggcgggg cagccaatcg tcgacgacat gctcctgggc cccgaggcgt acctgcagat
     1561 gtgggagcgc gcgatcggtg tggccggcag ctcgtgccgc acggacgtcg cggacctcac
     1621 gcccgaggac ttcgagcgtc tccgcaccgg gatgacggcc gaggaggtgc tccggacgct
     1681 gggccagccg cgctcgcgct ccggcaacga gttcgcctac tgcctcacct gggaccggac
     1741 gggaacggtg aggttcacct ccggtggcga ggtcgagagc tgggacaccg gcggcgcggt
     1801 cgcggtggtc ctggacgacg cgaacccgtc gacggggcag gtcaccgacg ccgcgaggtt
     1861 cgccgcgacc gtgacggacg ggtcgggtgc tcccgtcgcc ggtcagcccg tgacgttcac
     1921 cgtcggctcg gcgtccgcgc cggcgacgag tgacgagacc ggtcgcgccg tcgcgtcggt
     1981 ggtcctcgag ggcccggcgg gcgagcaggg cgtgacggtg tccgccggct cgctgacggg
     2041 atcggccccc ttcacggtga cgcgcgagga cacgctgctc acgctgttcg acgcggtggc
     2101 gagcgggaac gaccccgcga tggcgcgggc aggcttggcg gaggacggcg ggatggtcgc
     2161 gggccggacg atctcgttct acgtcgcgga caaggtgggg ggccgcaaga gcgcgacgtt
     2221 gatcggcacc gcggtcacgg acgtgaacgg gatggccgcg atcgaggtcc cgcagcgcta
     2281 cgtgtcgagc tcacgccgca cgatcacggc gacgttcgca ggcgacggcg acttcctgcc
     2341 cgcgtctggg acggcgtcgg cctaccgcgg ctagatcgcg gtcgggtggc gcggctcgta
     2401 gagcatcacc tcgacgccgc ccggtagcac cagcgtcgtc gtaatgcccc agccctcgtc
     2461 ctcgggctcg ccccggacct cgacgccgcg ctcacgcagc tccgcgacgg tcgcgttcac
     2521 gtcgtcgcac atgaacgtga gctggtgccg ggtgccgctg tcgtaggtcg ggccctcggc
     2581 ggggtggatc ccgagctcgg ccgggggcag ggcgaagatc agccatccgt cgcccgagtc
     2641 gacgtgtgag aacccgaaga cgtcgcgcag caccgcgcgc agctcgtcgg gctcgctcgt
     2701 gtacagcagc atgtgggtcc ctgtgatcgc catggcccca ccgtacgatc cggccatgga
     2761 gcctcgtgac gtgatcgtcc tcggcggagg gcccgccggg tacgcgtgtg cgcttcgcct
     2821 cagcgacctc gggctctcgt gcgtcctcgt cgaggcggcc gacctcggcg gcacctgtct
     2881 ccatcgcggc tgggtcccga cgcgcgccat gagcgaggtc gccgcgatcg ccgacacgct
     2941 ggcgcgcaag gccgagcggt gggggatcca cgccgtccta gagaaggtcg actacgcccg
     3001 cttgctcgcg acccgggacg agatcgtcgc acgcaaccac gtctcgatcc gccatcacct
     3061 cgaaagggct cgcgtcgacg tcgtcgccgg ttacg
//
LOCUS       DASQDO010000485         8124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1092051, whole genome shotgun sequence.
ACCESSION   DASQDO010000485 DASQDO010000000
VERSION     DASQDO010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092051"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1198
                     /locus_tag="VEV43_13345"
     CDS             155..1198
                     /locus_tag="VEV43_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYP24550.1"
                     /translation="MAARAPERPSAGVVFRNVLIVAAAVLLIYCLYLLRKLLMLVFIA
                     LFLAVGMDPAIRRLQGWGMKRGQAVAAILLAIVAFLAGFVAAVVPPLVDQVTEFATNL
                     PQYVRDLAENNERIREYVTEQNIAERLQDATKDIPAQIGSSFGTVVGVAGSVLATIFN
                     AITVLVLTIYFSLSFTRMREGSLRLIPKSKRERVQSILDPVLTKIGGYIAGNVVISIV
                     AGIVSFVFLAIAGVPFPVALALWVAIADLIPLVGATFGAVPAVVVAFFDSPGTGFATL
                     AFFAVYQQIENYLVAPRVMTKTVDLSPAAVLVAALGGGALLGVPGVLMAIPAAAAMKL
                     IANEIVVPWAERS"
     gene            complement(1195..1971)
                     /locus_tag="VEV43_13350"
     CDS             complement(1195..1971)
                     /locus_tag="VEV43_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYP24551.1"
                     /translation="MSDSFYVPLGDGVFRSTAWTTGPWGPDAQHAGPPSALLGRAIEG
                     AAPAGAHIARVTSEILRPVPIEDVKVEARVRRPGRSVALLEATLTAGAVEVMRASGWA
                     IGTTDVDLPADAAPRAEPQRPPDALPNLFEDRAETDYIAAMEWRFVSGSFFELGPATA
                     WLRMKLPLVQDEEPTPLQRVLTAADSSSGISGALDFSQWVYVNPDLTVHLHRALEGQW
                     VCLDAVTTPERNGVGLTAAALYDRRGPVGRSAQSLFVARR"
     gene            complement(1968..2522)
                     /locus_tag="VEV43_13355"
     CDS             complement(1968..2522)
                     /locus_tag="VEV43_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HYP24552.1"
                     /translation="MEPVFTVGHGTRTTEELAALLSGADATLLVDVRRFPGSRRHPHF
                     AREALEESLPALGVEYDWRGEELGGRRSRTKDSATRHPAWRNDAFGAYADYMDTPEFR
                     TALERLEAEARNGLRLAVMCAETLWWRCHRRLIADALALRGAEVVHLIQPGTTSVHPP
                     NEWARLDDDRYPVYDVGVTGELPL"
     gene            complement(2527..2922)
                     /locus_tag="VEV43_13360"
     CDS             complement(2527..2922)
                     /locus_tag="VEV43_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HYP24553.1"
                     /translation="MTQHSLDPESLPYAPEKLPLKDEVGGPGFFAVDMRAGRVVSVED
                     FPEARAPSWKVSVDFGPVIGVLTTSAKIKNYTREELEGRMVVGAVNLGTRRIAGFKSQ
                     FLVLGALDPDGTVRLLQVEDSVQPGAPIA"
     gene            2954..3757
                     /gene="mutM"
                     /locus_tag="VEV43_13365"
     CDS             2954..3757
                     /gene="mutM"
                     /locus_tag="VEV43_13365"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HYP24554.1"
                     /translation="MPELPEVESVRRQLDPELSGRRALAVWTDPHPAIPREFQDVDML
                     EGHEIESVGRRGKFLIAPLDGGLELVMHLGMTGSFRFDVQDDYARATVDLDDGRRLVF
                     RDVRRFGRMAVVPAGDYHSLPTLAKLGPEPLSDDFDPERFAAELARTSSPVKPFLLSQ
                     RPVAGVGNIYADEALWLARINPRSRAVGPARARALHGAIREVLAGAIEREGTTFSDYR
                     MVNGESGRNAGFLIAYGRGGTPCPRCKTPLRKIVLGGRGTTYCPTCQRR"
     gene            3767..4153
                     /locus_tag="VEV43_13370"
     CDS             3767..4153
                     /locus_tag="VEV43_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24555.1"
                     /translation="MKLTHVRLLTGDVQRLLGFYRDVLGLEVTLDAGVYYELGAGNVL
                     VGLFQRDLMGQMIGAELPPPGRSDAAVMTFAVDDVDEAARALRERGAQLVSEPKDYEV
                     AFLRVAHLRDPDGNLIEINAPLRGAS"
     gene            4150..5271
                     /locus_tag="VEV43_13375"
     CDS             4150..5271
                     /locus_tag="VEV43_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24556.1"
                     /translation="MKEIVLASMLAAAVTVPAHAAAESCGGRPATIVGSAKGEKITGT
                     KFADVIVGLGGNDVIKSLGGGDFVCGGDGKDKIQVANGHDNVWGEGGNDTMQGGRGDD
                     RLDGGGDVDTASYKDSDSPVDANLSIRSAIGEGADALVDVERLIGSEHGDTLTGDDTK
                     NSITGLGGPDVIEGLGGIDIQKSGVGDDRIHGGDGIDSHYGGPGDDFMDGGDGSDTPS
                     FKFSAAPVTVDLNEGTATGEGNDTFINMEIVAGSRHGDTFIGDETANTFYPLGGDDTV
                     DGGEGFDFVVFVQSPAAVVVDLLAGTVTGGEGDDSLTSIEGAVGTAYDDELYGTDADD
                     AFYGSGGDDTLDGRGGSDFLSGGPGTDSCANGEELDGCE"
     gene            complement(5332..5799)
                     /locus_tag="VEV43_13380"
     CDS             complement(5332..5799)
                     /locus_tag="VEV43_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24557.1"
                     /translation="MKKLVAGATIAILIAAPVQSALAAKKKAAHQHVEGTIAVPQGGN
                     AAATCVYRTQRTLLTAGGPNGVVGYTFDVDPATAGKKFKLEAGSGVGMDISFYADMGD
                     VTNPSAAPANYAYETPGPGGESGTVPAGYPLAFVCMTEGQNGTFSYAAGKGVK"
     gene            complement(5894..6070)
                     /locus_tag="VEV43_13385"
     CDS             complement(5894..6070)
                     /locus_tag="VEV43_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24558.1"
                     /translation="MTEAEQAAYNEFVLRLVRVCLDAQQRRNEEIRRSVDRIRELIEH
                     APFELAPRRRGLFR"
     gene            complement(6063..6491)
                     /locus_tag="VEV43_13390"
     CDS             complement(6063..6491)
                     /locus_tag="VEV43_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24559.1"
                     /translation="MWVGWSRELWHLVVAFALVAGLAGLAAAGLWPFDAVAVGSGDEA
                     SIAERALGDRLTIGLIRTAVLAVVSYLVISVPALVIDRRWIQGLTTSGLTTDPQAAER
                     WKEELVAELARLGGPSGGEIDPAEIAARIVRWDDEEEADD"
     gene            complement(6547..>8124)
                     /locus_tag="VEV43_13395"
     CDS             complement(6547..>8124)
                     /locus_tag="VEV43_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HYP24560.1"
                     /translation="LLFVLVTGLLGMALFHMFASFVRAHLLLNVRTQLDLRISMGFIH
                     HLVALPYSFFLQRSSGALMMRLNSNSTVREILTTGAMSTMLDGTLVSRYLVLIMVQNV
                     RMGLVVIGVGLLQVTVMMISHRRNQRLMSETLVAQARSQGYLVQIFGGIETLKSVGAE
                     ERAVEEWTNFFVDEVNATLQRGKLNAAVDSVMGSLRVASPLVILGVGALSVLNGNLSL
                     GQMLALNALAAGFLTPLANLITTGLQMQLLGSYMSRINDVLNTDPEQASKEGLKAPQL
                     KGAVRLENVSFRYSAGSPDVVKDVTLEIEPGQSIAIVGRSGSGKSTLAHLILGLYPPT
                     VGRVLYDGQPLDELDLAWVRRQLGIVPQDAFLFGTSIRKNIALSEPEMPLERVERAAQ
                     LAAIHDEIVKWPMKYETVLADGGATLSGGQRQRIALARALVHEPKILLLDEATSHLDV
                     ETEGVVHRNLSQLGCTRIVIAHRLSTVEKADLILVMDDGRFVERGTHDELLSARGRYW
                     QLVASQGESLVPRAAKP"
BASE COUNT         1240 a         2780 c         2863 g         1241 t
ORIGIN      
        1 gccgatcgtg atgatgtcgg cgcactcgag cccccccacg ctgcgcaagg ccgaggtcat
       61 cgggtcgcac gactacctca tcaagccctt caagcaagac gcgctgctcg cgatcatcga
      121 gaagcacctg cagaccgacc ggtaagctcc cggcatggct gcgcgggcac cggagcgacc
      181 ttcggcgggc gtcgtcttcc gcaacgtcct catcgtcgcg gccgcggtcc tgctgatcta
      241 ctgcctctac ctcctgcgca agctgctgat gctcgtgttc atcgcgctgt tcctcgcggt
      301 cggcatggat ccggcgatcc ggcgtctcca gggatggggg atgaagcggg ggcaggccgt
      361 cgcggccatc cttctcgcga tcgtcgcgtt cctcgccggt ttcgtggccg ccgtcgtgcc
      421 ccctctggtc gaccaggtca ccgagttcgc gacgaacctc ccgcagtacg tccgtgacct
      481 cgcggagaac aacgagcgca tccgcgagta cgtgaccgag cagaacatcg cggaacgcct
      541 ccaggacgcg acgaaggaca tccccgcgca gatcgggagc tcgttcggca ccgtagtggg
      601 cgtcgcgggt tcggtgctcg caacgatctt caacgcgata acggtcctcg tgctcacgat
      661 ctacttctcg ctgagcttca ccaggatgcg cgaggggagc ctgcggctga tccccaagag
      721 caagcgcgag cgggtgcaga gcatcctgga cccggtcctc acgaagatcg gcggctacat
      781 cgcaggcaac gtcgtgatct cgatcgtggc cggcatcgtg tccttcgtct tcctggcgat
      841 cgccggggtc ccgttccccg tagcgctggc gctctgggtc gcgatcgccg acctcatccc
      901 gctcgtcggc gcgaccttcg gcgcggtccc cgcggtggtc gtcgccttct tcgactcgcc
      961 cggcaccggc ttcgcgacgc tcgcgttctt cgccgtctac cagcagatcg agaactacct
     1021 cgtcgcgccg cgggtgatga cgaagaccgt cgacctgtcg ccggcggccg tgctcgtcgc
     1081 cgccctcggc ggcggtgccc tgctgggcgt gcccggcgtg ttgatggcga tccccgcggc
     1141 cgccgcgatg aagttgatcg cgaacgagat cgtcgtgccc tgggcggaga ggtcctagcg
     1201 gcgcgcgacg aacagcgact gagcgctgcg gccgacgggc ccgcgccggt cgtacagggc
     1261 ggctgcggtg agaccgacgc cgttccgctc cggcgtagtc acggcgtcga ggcacaccca
     1321 ctggccttcg agcgcacggt gcaggtggac ggtcaggtcg gggttcacgt agacccactg
     1381 cgagaagtcg agcgcgcccg agatcccgct cgacgagtcc gccgcggtga ggacgcgctg
     1441 gagaggcgtc ggctcctcgt cctgcaccag cggcagcttc atccggagcc acgcggtggc
     1501 cgggccgagc tcgaagaacg agccgctcac gaaccgccac tccatcgcgg cgatgtagtc
     1561 ggtctcggcc ctgtcctcga agaggttcgg gagagcgtcg ggcgggcgct ggggctcggc
     1621 gcggggcgcg gcgtccgcgg gcaggtcgac gtcggtagtt ccaatggccc agccgctcgc
     1681 acgcatcacc tcgacggcgc cggcggtgag cgtcgcctcg aggagcgcga cgctccggcc
     1741 cggccgcctc acgcgcgcct cgaccttcac gtcttcgatg ggtaccggcc gcaggatctc
     1801 cgaagtgacg cgcgctatgt gcgcgcccgc gggagcggcc ccctcgatcg cgcggcccag
     1861 cagcgcggac gggggcccgg catgctgcgc gtccggcccc cacggccccg tcgtccacgc
     1921 ggtcgagcgg aagacgccgt cgccgagggg cacgtagaag gagtcgctca cagcggcagc
     1981 tcgccggtca cgccgacgtc gtagaccgga tagcggtcgt cgtcgaggcg cgcccactcg
     2041 ttgggcgggt gcacggaggt cgtgcccggc tggatcaggt ggaccacctc ggccccgcgc
     2101 agagcgagag catcggcgat cagcctcctg tgacagcgcc accacagggt ctccgcgcac
     2161 atcaccgcga ggcgtaatcc gttccgggcc tcggcttcga gccgctcgag cgcggtgcgg
     2221 aactccggcg tgtccatgta gtcggcgtag gccccgaacg cgtcgttgcg ccacgcgggg
     2281 tgccgcgtgg cgctgtcctt cgtgcgcgac cgtcgtccgc cgagctcctc gccccgccag
     2341 tcgtactcga caccgagcgc cggcagcgac tcttcgagcg cctcgcgggc gaagtgcgga
     2401 tgacgacggg atccggggaa cctccggacg tcgacgagca gggtggcgtc cgctccgctc
     2461 agcaacgcgg ccagctcctc ggtggtccgg gtcccgtgcc cgacggtgaa gacgggttcc
     2521 acgcggtcag gcgatcggcg cgccgggttg caccgagtcc tccacctgca gcagccggac
     2581 ggtcccgtcc gggtcgaggg cgccgaggac gaggaactgg ctcttgaagc ccgcgatgcg
     2641 gcgggtgccg aggttgacgg cgccgacgac catgcggccc tcgagctcct cgcgcgtgta
     2701 gttcttgatc ttggcgctcg tcgtcagcac gccgatcacc ggaccgaagt cgaccgacac
     2761 cttccacgag ggtgcccgcg cctccgggaa gtcctcgacg gagaccacgc ggcccgcgcg
     2821 catgtcgacc gcgaagaaac cgggcccgcc gacctcgtcc ttgagaggga gcttctcggg
     2881 cgcgtaaggc agcgactcgg gatcgagtga atgctgggtc atgacgcgac tctacggggc
     2941 tacgctcggc gtcgtgcccg agctccccga ggtcgagtcc gtccgtcgcc agctggaccc
     3001 cgagctgtcc ggccggcgcg ccctcgcggt atggaccgac ccgcacccgg cgatcccgcg
     3061 cgagttccag gacgtcgaca tgctcgaagg gcacgagatc gagtcggtgg gcaggcgggg
     3121 caagttcctg atcgcgccgc tcgacggggg cctcgagctc gtcatgcacc tcgggatgac
     3181 gggatcgttc cgcttcgacg tgcaggacga ctacgcgcgg gcgacggtcg acctcgacga
     3241 cggccgccgg ctcgtgttca gggacgtccg gaggttcgga cggatggcgg tggttcccgc
     3301 cggggactat cactcgctgc cgactctggc gaagctcggc ccggagcccc tgtcggacga
     3361 cttcgacccc gagcgcttcg cggcggagct cgcgcggacg tcgtccccgg tcaagccctt
     3421 cctgctgagc cagcggccgg tcgccggcgt cggcaacatc tacgcggacg aagcgctgtg
     3481 gctcgcgcgg atcaacccgc gctcgcgcgc agtcgggccg gcgcgggctc gggcgctgca
     3541 cggcgcgatc agggaggtgc tggccggagc gatcgagcgc gagggcacga cgttctcgga
     3601 ctaccgcatg gtcaacggcg agtccgggcg caacgcgggg ttcctgatcg cgtacgggcg
     3661 cggggggacg ccgtgcccgc gctgcaagac gccgttgcgc aagatcgtcc tcgggggccg
     3721 cgggacgacc tactgcccca cttgtcaaag acgttaggag gacgggatga agctcacgca
     3781 cgtacgactt ctgaccggcg acgtccagcg gttgctcggc ttctaccgcg acgtgctcgg
     3841 cctggaggtg acgctcgacg cgggcgtcta ctacgagctc ggtgcgggga acgtcctcgt
     3901 cgggctgttt caacgcgacc tgatggggca gatgatcggt gccgagctgc ctccgccggg
     3961 ccggtccgac gccgcggtca tgacgttcgc ggtcgacgac gtcgacgagg cagcgagggc
     4021 cctgcgggag cggggcgcgc agctcgtctc cgagccgaag gactacgagg tcgcgttcct
     4081 gcgcgtggcg cacctgcggg accctgacgg caacctgatc gagatcaacg cgccgctgag
     4141 gggggcttcg tgaaagagat cgtgctcgca agcatgctgg ccgcggccgt taccgtgccg
     4201 gcacacgccg ccgcggaatc gtgcggcggc cgcccggcga cgatcgtcgg ctcggccaag
     4261 ggcgagaaga tcaccggcac gaagttcgcc gacgtgatcg tcgggctcgg cgggaacgac
     4321 gtgatcaagt cgctcggcgg cggcgacttc gtctgcggcg gtgacggcaa ggacaagatc
     4381 caagtcgcca acggccacga caacgtctgg ggcgaaggcg gcaacgacac gatgcagggg
     4441 ggccgcgggg acgaccggct cgacggcgga ggtgacgtcg acaccgcgag ctacaaggac
     4501 tcggactcgc ccgtcgacgc gaacctgtcg atccggtcgg cgatcggcga aggcgccgac
     4561 gcgctcgtgg acgtcgagcg gctgatcggc tcggagcacg gcgacacgct caccggcgac
     4621 gacacgaaga actcgatcac cggtctcgga ggccccgacg tcatcgaggg cctcggcggg
     4681 atcgacatcc agaagtccgg cgtcggcgac gaccgcatcc acggcggcga cgggatcgac
     4741 tcgcactacg gggggccggg agacgacttc atggacggcg gcgacggcag cgacacgccg
     4801 tcgttcaagt tctcggccgc ccccgtgacg gtcgacctga acgagggaac ggccacgggc
     4861 gagggcaacg acacgttcat caacatggag atcgtggcgg ggtcgcgtca cggcgacacg
     4921 ttcatcgggg acgagaccgc gaacacgttc tatccgctgg gcggcgacga caccgtcgac
     4981 gggggagagg gcttcgactt cgtggtgttc gtgcaatcgc ccgcggcggt cgtcgtggac
     5041 ctgctggccg ggacggtgac cgggggtgag ggcgacgact cgctcacgtc gatcgagggc
     5101 gcggtcggca cggcctacga cgacgagctc tacggcacgg acgccgacga cgcgttctac
     5161 gggagcggtg gcgacgacac gctcgacggc cgcggcgggt cggacttcct cagcgggggg
     5221 ccgggcaccg acagttgcgc gaacggcgag gagctcgacg gctgcgagtg agcgcaagga
     5281 aaagccccgg gccggtgttg cgcccggggc tttatttcgg tcttttgatc cttacttgac
     5341 gcccttgccc gccgcgtagc tgaaggtgcc gttctggccc tcggtcatgc agacgaaggc
     5401 gagggggtag ccggcgggga cggtgccgga ctcgccgccg gggccggggg tctcgtaggc
     5461 gtagttcgca ggggcggccg aggggttcgt cacgtcgccc atgtcggcgt agaacgagat
     5521 gtccatgccg acgccgctgc cagcctcgag cttgaacttc ttgcccgccg tggcggggtc
     5581 gacgtcgaag gtgtagccga cgacgccgtt ggggccaccg gccgtcagga gcgtgcgctg
     5641 ggtgcggtag acgcaggtag ccgcggcgtt gccgccctgc gggacggcga tggtgccctc
     5701 gacgtgctgg tgggcggcct tcttcttggc ggcgagcgcc gactgcacgg gggccgcgat
     5761 gaggatcgcg atcgtggcgc ctgcgacgag cttcttcatg gcttcacctc gtggcgttgg
     5821 aatgggttga gactgaggtt cgccacgggg aaggccggtc ctgcgggggc cggttatgtc
     5881 cggtttgggg ggcctagcgg aagagtcccc ggcgccgggg cgcgagctcg aacggcgcgt
     5941 gctcgattag ctccctgatc ctgtccacgg accggcggat ctcttcgttc ctgcgctgct
     6001 gggcgtcgag acagacccgg accagcctga gcacgaactc gttgtaggca gcctgttcgg
     6061 cttcagtcat cggcttcctc ttcatcgtcc catcgtacga tccgagccgc gatctccgcg
     6121 gggtcgatct ctccccccga cggtcccccc aaccgcgcga gctcggccac cagctcctct
     6181 ttccaacgtt ccgctgcctg tgggtcggta gttaggccac tagtcgtcag gccctggatc
     6241 caccggcggt cgatcacgag cgcgggcacg ctaatcacca ggtacgacac cacggcgagg
     6301 actgcggttc tgatcaggcc gatcgtcaga cgatccccga gagcgcgttc ggcgatcgag
     6361 gcctcgtctc ccgagcccac ggcaacggca tcgaacggcc acaaccccgc ggccgcaagc
     6421 cccgcgagac ccgcgacgag cgcgaacgcc accacgagat gccacagctc ccggctccat
     6481 ccgacccaca tgccgccaac gctaggccac ggcccccggc cccgaagtgg ctcaggtcac
     6541 acgtgctcag ggcttcgcgg ccctcgggac cagcgactcg ccctgcgacg ccaccagctg
     6601 ccagtagcgg ccccgcgccg acaggagctc gtcgtgcgtc ccccgctcta cgaaccggcc
     6661 gtcgtccatg accaggatga ggtcggcctt ctcgaccgtg ctcagccggt gcgcgatcac
     6721 tatgcgcgtg cagccgagct gcgagaggtt gcggtgcacg acgccctcgg tctcgacgtc
     6781 gaggtggctc gtcgcctcgt cgagcagcag gatcttcggc tcgtgcacga gcgcccgcgc
     6841 cagcgcgatg cgctgacgct gccccccgga cagcgtcgcg cccccgtcgg cgaggaccgt
     6901 ctcgtacttc atcggccact tcacgatctc gtcgtggatc gccgcgagct gggcggccct
     6961 ctcgacgcgc tcgagcggca tctccggctc cgacaacgcg atgttcttgc ggatgctcgt
     7021 cccgaacagg aaggcgtcct gcggcacgat gccgagctgc cggcgcaccc aggcgaggtc
     7081 gagctcgtcc agcggctgcc cgtcgtacag cacgcggccg acggtcggcg ggtacaggcc
     7141 gaggatgaga tgcgcgagcg tcgacttgcc cgaccccgac cggccgacga tcgcgatgct
     7201 ctgacccggc tcgatctcga gcgtcacgtc cttcacgacg tcgggggagc cggcgctgta
     7261 gcggaacgac acgttctcca gacggaccgc tcccttcagc tgtggggctt tcaagccttc
     7321 tttcgaagcc tgctccgggt cggtgttcag cacgtcgttg atgcgggaca tgtagctgcc
     7381 gagcagctgc atctggagac cggtcgtgat caggttcgcg agcggcgtga ggaatcccgc
     7441 ggccagcgcg ttcagcgcga gcatctggcc gagcgagagg ttgccgttca gcacgctgag
     7501 cgcgccgacg ccgaggatga ccagcggaga ggcgacgcgc agcgatccca tgaccgagtc
     7561 gacggcggcg ttgagcttcc ctcgttgcag cgtcgcgttg acctcgtcga cgaagaagtt
     7621 cgtccactcc tcgaccgcgc gctcctcggc ccccacggac ttgagcgtct cgatcccgcc
     7681 gaagatctgg acgaggtacc cctgcgagcg cgcttgcgcg accagtgtct cggacataag
     7741 cctctggttg cggcggtgcg agatcatcat cacggtcacc tggaggagcc cgacgccgat
     7801 cacgacgagc cccatccgca cgttctgcac catgatcagc acgaggtagc ggctgacgag
     7861 cgtcccgtcg agcatcgtcg acatcgctcc ggtcgtcagg atctcgcgca cggtcgagtt
     7921 cgagttcaac cgcatcatca gggcgccgga cgagcgctgc aggaagaacg agtacggcag
     7981 cgccacgagg tggtggatga accccatcga gatcctgagg tcgagctggg tccggacgtt
     8041 cagcaacagg tgcgcgcgga cgaacgacgc gaacatatgg aacagcgcca tcccgagcag
     8101 gccggtgacg agcacgaaca agag
//
LOCUS       DASQDO010000486         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_113037, whole genome shotgun sequence.
ACCESSION   DASQDO010000486 DASQDO010000000
VERSION     DASQDO010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113037"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VEV43_13400"
     CDS             complement(<1..1252)
                     /locus_tag="VEV43_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24561.1"
                     /translation="MKRALAALAMLAAIVPAGAVRAQECSNVVVIGLAGVTWGDVQRV
                     QPPQILELAEEGAVGSVSVRTNTSRTTYASGFATIGAGARMDAPKSVGPAELGNKRAT
                     GVRVQGVDAIRELALAAGYSTSVPGALATTLADRPVAAIGSSALGAEPASPVGVDVPV
                     LLAAMDDHGLVDRAAVGGDVLEADPGSPFGVRTDLDVVEGALRDFLDDPCSVVFLDPG
                     DLVRADALARLGDESARDEALLSADRQIGAAAEALDRDDLLLVVTVTSPRSDDRTHLG
                     VAIAWGTGVYPGDSLESASTRKAGLVTLPDVAPTVLQHLEIARPTSMLGRPWFGRPHL
                     VDPVAEAVDLDEESVFSYGVQPGVATGFVVAQVAAYVVLLALFGLRRRGRAIPAGVPA
                     TALEVVALAVAAFPLASYLAMAGMT"
     gene            complement(1249..2343)
                     /locus_tag="VEV43_13405"
     CDS             complement(1249..2343)
                     /locus_tag="VEV43_13405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP24562.1"
                     /translation="MTAVRVLQVLGFSTGGISRHVAQVTQALDGNGFEVDVAGPDDLP
                     LKMPKPVLPVSIPSGPVRGHRAAVGRLRGIVGAGDYGIVHAHGLRAGIDAALAARGRC
                     PVVLTVHNVVHPDVAGRTKYLAYKQAETLAVGLTAHTFAVSAEIATHLRKHPGARGKV
                     EVLYLGIGDPPRVTRERSAVRAELGVAADERLVVTASRLSAQKALPVMFEALGAAATP
                     FVLALLGTGPQEEELRRAAAEHGIAERVRWLGFRPDVADYIAAGDVFCLSSVWEGVPL
                     AAQEAILLGVPVVATAVGGMPELVDDAVTGRLVPPRDPAALAAALRQVLASDALRKTF
                     ADGAKKALQKKFSTERMLARLAGEYRERAR"
     gene            complement(2340..>2529)
                     /locus_tag="VEV43_13410"
     CDS             complement(2340..>2529)
                     /locus_tag="VEV43_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24563.1"
                     /translation="IAAGGVMAALSWALPGDGLAPALVTLVAAGVAGAAAYGGLLWWV
                     KSRELGRVVALVRGARAS"
BASE COUNT          374 a          964 c          895 g          296 t
ORIGIN      
        1 gcgtcatccc ggccatcgcc aggtacgacg cgagcgggaa cgcggccacg gcgagcgcga
       61 cgacctccag cgccgtcgca ggcacgccgg ccgggatcgc gcggcccctg cgccggaggc
      121 cgaagagcgc gagcagcacg acgtatgcgg cgacctgggc gacgacgaac cccgtcgcca
      181 cccccggctg cacgccgtag gagaagacgg actcctcgtc gaggtcgacc gcctcggcca
      241 cgggatcgac taggtgcggc cgtccgaacc agggccggcc gagcatcgac gtcggccggg
      301 cgatctcgag gtgctgcaag acggtcggcg cgacatcggg cagcgtcacg agccccgcct
      361 tccgcgtcga agcagactcc aggctgtctc ccgggtagac gcccgtcccc caggcgatgg
      421 cgactcccag gtgcgtgcga tcgtccgagc gcggggacgt gaccgtcacg accaggagca
      481 ggtcgtcgcg gtcgagggct tcagccgccg cacctatctg gcgatccgcc gacaacagag
      541 cctcgtcgcg cgcggactcg tcgcccaacc gcgccagcgc gtctgctcgc acgaggtcac
      601 cggggtcgag gaagaccacg gagcacgggt cgtccaagaa gtcccggagg gcgccttcca
      661 cgacgtcgag atcggtacgc acgccgaatg gtgatcccgg atccgcttcg agcacatcgc
      721 cccccaccgc ggcacggtcg accagaccgt ggtcgtccat cgcggccagg aggaccggga
      781 cgtctacgcc aacgggggac gcgggctccg ctcccagggc cgacgagcca atcgccgcca
      841 ccgggcggtc cgcgagcgtt gtcgcgagcg ctccgggaac ggaggtgctg tagccggcag
      901 cgagcgcgag ctcgcggatg gcatccacgc cctggacgcg caccccggtc gccctcttgt
      961 tccccagctc ggcgggtccg acggacttcg gcgcgtccat ccgggccccc gcgccgatcg
     1021 tcgcgaagcc ggacgcgtac gtggtcctcg acgtgttcgt gcggaccgag accgatccga
     1081 cggccccttc ttccgcgagc tcgaggatct gaggaggctg gacacgctgc acgtcgcccc
     1141 acgtcacgcc tgcgaggccg atcacgacga cgttcgagca ctcttgtgcc cgaaccgctc
     1201 ccgcaggcac gatcgcggcg agcatcgcga gcgcggccag ggcgcgcttc atcgggcccg
     1261 ctcccggtac tcgcccgcga ggcgcgcgag catccgctcg gtcgagaact tcttctgcag
     1321 cgccttcttc gcgccgtcgg cgaacgtctt gcgcagagcg tcgctcgcga ggacctgacg
     1381 gagcgccgct gcgagcgcgg cggggtccct gggcgggacg aggcgccccg tcaccgcgtc
     1441 gtcgacgagc tccggcatcc cgccgacggc cgtggcgacg accgggacgc cgagcaggat
     1501 cgcctcctgt gccgcgagcg ggacgccctc ccacacggac gacaggcaga agacgtcgcc
     1561 ggcggcgatg tagtcggcga cgtccgggcg gaagccgagc caccgcacgc gttcggcgat
     1621 cccgtgctcg gcggcggcgc gtcgaagctc ctcttcctgc ggtcccgtcc ccagcagcgc
     1681 gaggacgaac ggcgtcgccg cggcccccag cgcctcgaac atgaccggga gcgccttctg
     1741 agcggagagc cgcgacgccg tgacgacgag cctctcgtct gcggccacgc cgagctccgc
     1801 gcgcaccgcg ctgcgctcac gcgtcacccg gggcgggtcg ccgatgccga ggtagaggac
     1861 ctcgaccttt ccccgggcac cgggatgctt gcggagatgc gtcgcgatct ccgccgacac
     1921 cgcgaacgtg tgggccgtga ggccgaccgc cagcgtctcc gcctgcttgt acgcgaggta
     1981 cttagtccgc cccgccacgt cggggtgcac gacgttgtgg acggtgagga cgacggggca
     2041 acggccgcgg gccgcgagcg ccgcgtcgat gcccgcgcgc aggccgtgcg cgtggacgat
     2101 cccgtagtcg ccggccccga cgatcccgcg caagcgcccg accgccgcac ggtgcccgcg
     2161 gacgggcccg ctcgggatcg agaccgggag caccggcttc ggcatcttca gcggcaggtc
     2221 gtcgggcccc gcgacgtcca cctcgaaccc gttgccgtcg agggcttgcg tgacctgcgc
     2281 gacgtggcgg gagatcccgc cggtcgagaa gccgaggacc tgcaggacgc ggaccgccgt
     2341 cacgatgccc gggccccgcg cacgagcgcg acgacgcggc cgagctcccg cgacttcacc
     2401 caccacaaca gaccgccgta ggcggcggcg ccggcgaccc ccgccgcgac cagcgtgacc
     2461 agagcgggcg cgaggccgtc acccggcagt gcccacgaca gcgccgccat caccccgccg
     2521 gcggcgatc
//
LOCUS       DASQDO010000487         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_235423, whole genome shotgun sequence.
ACCESSION   DASQDO010000487 DASQDO010000000
VERSION     DASQDO010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235423"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VEV43_13415"
     CDS             complement(<1..1082)
                     /locus_tag="VEV43_13415"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24564.1"
                     /translation="MKTRRYKIMFIVGLVVSFGGLGGVIAAGASPKLGLDLEGGISVI
                     LQATGEEVRPDVLNETVNLIRDRIDALGVAEPEVSVAGEDNVLIQLPGVEDEEAALEL
                     IGTTAQLTFRQVKAVYGPNDKKAPEITEEADSSVNDEVVVYPSADPQDKALYELEPAN
                     GLTGTIVEDAEAVPVSANQWTVSIDMNSQGADLWEDLTDRLACLRDEGEQVKSQVAIV
                     LDGKVESAAGMQDPATSGQGGGVECGVGIEGGETQIDVGEENEAKDLALVLTTGALPI
                     TLEQQEVRKVSPTLGRESLKTGLTAGAVGLGLVMIYVLLYYRTLGLVIWLGFFQFTAL
                     LYTALAILGESAGLSLTLAGVAGIIVS"
     gene            complement(1093..1830)
                     /locus_tag="VEV43_13420"
     CDS             complement(1093..1830)
                     /locus_tag="VEV43_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYP24565.1"
                     /translation="MSEAAGDAGPRPLEPAPGPEPIQPASPIGLAELQAHEGVQTFIS
                     LADRYLGEIGYTEHGFRHANLVAKIAYNVLHRLGYDERMAELGAMAGYLHDMGNFVSR
                     TMHSQTGAAITYDILRDIGLPYGEIGVVLGAIGNHEEEFGNPVNPAAAALIVADKSDV
                     HRSRVRGKDPQLFDIHDRVNHAVEHSFLRVDADARTLTLELTIDTELAGVMEYFEIFL
                     SRMVMCRRAGEFLGCLFKIDINGSKLL"
     gene            complement(1827..2141)
                     /gene="yajC"
                     /locus_tag="VEV43_13425"
     CDS             complement(1827..2141)
                     /gene="yajC"
                     /locus_tag="VEV43_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYP24566.1"
                     /translation="MNEGIGQIVFLALLIAVFYFVLIRPQKKRAEMHQQLISNVDVGD
                     EIITIGGLYGRVTAMGDDEFEMEPSPGTRLRFVKSAIARRVTEELETGGETADEIGET
                     GV"
     gene            complement(2244..>2682)
                     /locus_tag="VEV43_13430"
     CDS             complement(2244..>2682)
                     /locus_tag="VEV43_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HYP24567.1"
                     /translation="ILDVSLDEEGALEIARRSRGTPRIANRLLRRVRDFAQVRAQGAI
                     DGDVARDALAVFKVDEKGLDKTDGAILEALVRRFGGGPVGLGTLAVSVGEEPDTIEDV
                     YEPFLMQLGLLRRTPRGRVATEAAFAHVGAPLPKGAAARLFES"
BASE COUNT          382 a          939 c          903 g          458 t
ORIGIN      
        1 gagacgatga tcccggcgac tccggcgaga gtcagcgaca atcccgcgga ctcgcccagg
       61 atcgcgagag cggtgtagag gagcgccgtg aactggaaga acccgagcca gatcacgagc
      121 ccgagggtcc ggtagtagag aagcacgtag atcatcacga gcccgagccc gaccgcacct
      181 gccgtgagcc ccgtcttcag ggattcgcgg ccgagggtcg gcgagacctt gcgcacctcc
      241 tgctgctcga gcgtgatcgg cagcgcgccg gtcgtcagca cgagggccag gtccttcgcc
      301 tcgttctcct cgcccacgtc gatctgagtt tcaccaccct cgatcccgac gccgcactcg
      361 acgcccccgc cctgtccgga ggtggccggg tcctgcatgc cggcggcgga ctcgaccttg
      421 ccgtcgagga cgatcgccac ctggctcttg acctgctcgc cctcgtcacg caggcacgcg
      481 agcctgtcgg tcaggtcctc ccacaggtcg gcgccctgtg agttcatgtc gatgctgacc
      541 gtccactggt tggccgagac cggcacggcc tcggcgtcct cgacgatggt cccggtgagg
      601 ccgttcgcgg gctcgagctc gtagagggcc ttgtcctgcg gatcggccga cggatacacg
      661 acgacctcgt cgttcaccga cgagtccgcc tcctcggtga tctcgggcgc cttcttgtcg
      721 ttgggcccgt agacggcctt gacctgccgg aacgtcagct gtgcggtcgt gccgatgagc
      781 tcgagcgccg cttcttcgtc ctcgacgccc gggagctgga tcaggacgtt gtcctctccc
      841 gccacgctga cctcgggctc ggccacgccg agcgcgtcga tgcggtcgcg gatgaggttg
      901 acggtctcgt tgaggacgtc gggccgcacc tcctcgccgg tggcctggag gatgacgctg
      961 atcccgccct cgagatcgag gccgagcttc ggcgaggccc ccgccgcgat gacgccgccg
     1021 agcccgccga acgacacgac gagcccgacg atgaacatga tcttgtaccg cctggtcttc
     1081 atgcttcttc cctcataaga gcttgctccc gttgatgtct atcttgaaca ggcacccgag
     1141 gaactccccg gccctcctgc acatcaccat ccgcgacagg aagatctcga agtactccat
     1201 gacgcccgcg agctccgtgt cgatcgtgag ctccagggtg agagtacgcg catccgcgtc
     1261 gactcgcagg aacgagtgct cgacggcgtg gttgacccgg tcgtggatgt cgaacagctg
     1321 ggggtccttc ccccggaccc tcgagcggtg gacgtcggac ttgtcggcga cgatcagcgc
     1381 tgccgcagcg gggttgacgg ggttgccgaa ctcctcctcg tggttgccga tcgccccgag
     1441 gaccacgccg atctcgccgt agggcagccc gatgtcgcgc aggatgtcgt acgtgatcgc
     1501 ggcccccgtc tgcgagtgca tcgtccgcga cacgaagttg cccatgtcgt ggaggtagcc
     1561 ggccatcgcg cccagctcgg ccatccgctc gtcgtagccg agccggtgca ggacgttgta
     1621 ggcgatcttc gccacgaggt tcgcgtggcg gaagccgtgc tccgtgtagc cgatctcgcc
     1681 gaggtaccgg tcggcgagcg agatgaacgt ctggacgccc tcgtgggcct ggagctccgc
     1741 gaggcctatc ggagacgccg gctgtatcgg ctcggggccc ggggcgggct cgagtggtcg
     1801 cggcccggcg tcgccggccg cttcgctcat accccggtct ctccgatctc gtccgcggtc
     1861 tcgccgccgg tctcgagctc ttcggtcacg cggcgtgcga tcgcggactt gacgaagcgc
     1921 agcctcgtcc cgggcgacgg ctccatctcg aactcgtcgt cgcccatcgc cgtgaccctg
     1981 ccgtagaggc cgccgatcgt gatgatctcg tcgccgacgt cgacgttcga gatcagctgc
     2041 tggtgcatct cggcgcgctt cttctgcggc ctgatcagga cgaagtagaa gaccgcgatc
     2101 agcagcgcga ggaagacgat ctgccctatg ccctcattca cttgcttcac ttccttccga
     2161 ggcgtctgtg gcgccgattc ccagcccggc gcgaacggag ccgcttgccg cggctccggg
     2221 ggcatcctac cgggggaccg ccgctaggac tcgaacaggc gggccgcggc ccccttgggc
     2281 agcggcgctc cgacgtgcgc gaaggcggcc tcggtcgcga cccggcccct cggcgtacgc
     2341 cggagcaggc cgagctgcat gaggaagggc tcgtagacgt cctcgatcgt gtccggctcc
     2401 tcgcccacgg acaccgcgag ggtgccgagg ccgacggggc ctccgccgaa ccgccgcacg
     2461 agggcttcga ggatcgcgcc gtcggtcttg tcgaggccct tctcgtcgac cttgaagacg
     2521 gcgagcgcgt cgcgggcgac gtcgccgtcg atcgcgccct gggcgcgcac ctgtgcgaag
     2581 tcgcggaccc tgcgtagcag ccggttcgcg atgcgggggg tcccgcggga gcggcgggcg
     2641 atctcgagcg cgccctcctc gtcgagggag acgtcgagga tc
//
LOCUS       DASQDO010000488         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_908358, whole genome shotgun sequence.
ACCESSION   DASQDO010000488 DASQDO010000000
VERSION     DASQDO010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908358"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VEV43_13435"
     CDS             complement(<1..481)
                     /locus_tag="VEV43_13435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYP24568.1"
                     /translation="MAPGALPPRLAGWYARRTFTGSPDPADVARAKSARGLKVSVCLP
                     ARNEEATVGAICSVIDRELVEGAGLVDELIVIDSGSTDGTAATARSGGARVVPATEPG
                     QGQALWESLAVATGDLVVWLDSDTREFSAHFVTNLVAPLVADDSIALCKGFYRRPLAT
                     "
     gene            622..1065
                     /locus_tag="VEV43_13440"
     CDS             622..1065
                     /locus_tag="VEV43_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP24569.1"
                     /translation="MAKIHIVDDDADIRRLLSYHVIEEGHEVSVGRDGEEILQTIAND
                     QPDLLVLDLMMPKLDGITVLERMDESGMRGATRIVVLSANGAEMDRALGLGADHYMTK
                     PFDPKDLIEVVTELLALSRVELDLRRDHERVKARLMSRLDSLYGS"
     gene            1068..1517
                     /locus_tag="VEV43_13445"
     CDS             1068..1517
                     /locus_tag="VEV43_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP24570.1"
                     /translation="MARILVVDDSPEMRHLLTSILVEEGHKVVVAPDGQKALDMVVAD
                     PPDIMVLDIMMPAMDGYMVLKELRATGIRDTTKILLLTAKTSEHDWVRGYKLGADQYL
                     TKPFDIQELTAAIETLLVSSKEQLKTRREEELNKAQLLSRLESLFEA"
     gene            1679..2275
                     /locus_tag="VEV43_13450"
     CDS             1679..2275
                     /locus_tag="VEV43_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYP24571.1"
                     /translation="MRRPRQDRGDSTRELVPFELVERCRGGDEGAWKELVELTHREVY
                     TLCLRILNDPSDAAEATQDAYLKAWRGLRGFRGDAQFTTWLYRVASNAAISKHRSRKR
                     RRVYEVGAEDELLSAMPAAGSVEGTAEVRRDVQELEGAIRNLPDHYRSAVVLRDVYGL
                     SIEEIAKELKITETAAKVRVHRARKKLKETLHPTGDET"
     gene            2272..2628
                     /locus_tag="VEV43_13455"
     CDS             2272..2628
                     /locus_tag="VEV43_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24572.1"
                     /translation="MSDTRCEEVRAALPELVDEPGMTLAVRRHLSRCPECEGELEAYR
                     SLRAATAQLASATSTPPPGLVESLISIPAEGTRLEDLRAHVSRHRRRYATGAAVAMGA
                     AGAALWKSRRRGLVTA"
     gene            2657..2730
                     /locus_tag="VEV43_13460"
     tRNA            2657..2730
                     /locus_tag="VEV43_13460"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2691..2693,aa:Trp,seq:cca)
BASE COUNT          465 a          950 c          953 g          391 t
ORIGIN      
        1 cggtggcgag cggccgccgg tagaaaccct tgcacagcgc gatcgagtcg tccgcgacca
       61 gcggcgcgac gaggttcgtc acgaagtggg ccgagaactc cctcgtgtcc gagtcgagcc
      121 atacgacgag gtcacccgtc gccacggcca ggctctccca cagcgcctga ccctgaccgg
      181 gctcggtcgc cgggacgacc cgggcccctc cgctgcgggc cgtggcggcg gtcccgtccg
      241 tcgaaccgct gtcgatcacg atcagctcgt cgacgagccc ggccccctcc acgagctccc
      301 ggtcgatcac cgagcagatc gctccgacgg tggcctcctc gttccgggcc ggcaggcaca
      361 cgctgacctt caggccccgg gccgacttcg cccgggcgac gtccgccggg tcgggcgagc
      421 ccgtgaacgt ccggcgcgcg taccagccgg cgagccgtgg gggtagcgct ccgggggcca
      481 tcggcccagc gtacggcggc ttcccgggtc gaagggaagt tgtcgcggac cccggtcaag
      541 gacccctccg gagacgccga tagaccactt ggacgacgca ggccagatca gaccgcgagg
      601 ggcaacgacg cggaggacca gatggcgaag atccacatag tcgacgacga cgccgacatc
      661 cggcgtctgc tctcgtacca cgtcatcgag gaggggcacg aggtctcggt cggccgcgac
      721 ggcgaggaga tcctccagac gatcgcgaac gaccagcccg acctgctcgt cctggacctc
      781 atgatgccga agctcgacgg gatcaccgtc ctcgagcgga tggacgagag cggcatgcgc
      841 ggcgcgaccc gcatcgtcgt gctcagcgcc aacggcgcgg agatggaccg ggccctcggg
      901 ctcggcgccg accactacat gacgaagccg ttcgacccga aggacctgat cgaggtcgtc
      961 accgagctgc tcgcgctctc gcgggtcgag ctcgacctcc gccgtgacca cgagagggtc
     1021 aaggcccggc tgatgtcgcg tctcgactcg ctctacggga gctaggcgtg gcgaggatcc
     1081 tggtcgtaga cgacagcccg gagatgcggc acctcctgac gagcatcctc gtcgaggagg
     1141 ggcacaaggt cgtagtggcg cccgacggtc agaaggcgct cgacatggtc gtcgccgacc
     1201 cgcccgacat catggtgctc gacatcatga tgccggcgat ggacggctac atggtgctca
     1261 aggagctccg tgcgaccggc atccgcgaca cgacgaagat cctcctcctg acggcgaaga
     1321 cttcggagca cgactgggtc cgcggctaca agctcggcgc cgaccagtac ctcacgaagc
     1381 ctttcgacat ccaggagctc accgccgcga tcgagacgct cctcgtgagc tcgaaggagc
     1441 agctgaagac ccgccgcgag gaggagctca acaaggccca gctcctctct cggctggaga
     1501 gcctgttcga ggcctagata gtcgctccgg cgaccgctgc ggggcgatgt gccccgctcc
     1561 gctcctccac accgtttctt agtcgtcgct ccgggggcac atcgccctcc gctgtatctc
     1621 gcgggctgat cacgcgtcga aaggtcacaa ggacctcgcg ccggtaacct ccttctacgt
     1681 gaggcgtcct aggcaggacc ggggtgattc gacccgggag ctcgttccct tcgaactcgt
     1741 cgaacgatgc cgtggtggcg acgagggcgc gtggaaggag ctcgtcgagc tgacgcaccg
     1801 ggaggtctac acgctgtgcc ttcggatcct caacgatccc agcgacgccg ccgaggccac
     1861 gcaggacgcg tacctcaaag cgtggcgggg tctgcgtggg ttccggggag acgcgcagtt
     1921 cacgacctgg ctgtaccggg tggcgagcaa cgcggccatc agcaagcacc ggagcaggaa
     1981 gcgcaggcgc gtgtacgagg tgggagccga ggatgagctg ctgtcggcga tgccggccgc
     2041 cgggtcggtc gaggggactg ccgaggtccg gcgcgatgtc caggagctcg agggcgccat
     2101 ccgcaacctg cccgaccact accgctccgc ggtggtcctg cgggacgtgt acggcctgtc
     2161 gatcgaggag atcgcgaagg agctgaagat cacggagacc gcggcgaagg tgcgtgtgca
     2221 ccgggcccgc aagaagctga aagagaccct gcacccgacc ggagacgaga catgagcgac
     2281 actcgctgcg aagaggtccg agccgcactt cccgagctcg tggacgagcc cggcatgacg
     2341 ctggccgtac gccggcacct gtcgcgctgt cccgagtgcg agggcgagct cgaggcttac
     2401 cggtcactcc gcgcggcgac cgcgcagctg gcgtccgcga cgtcgacgcc cccgcccggg
     2461 ctcgtcgagt cgctgatctc catcccggcc gaaggcacgc gcttggaaga cctgcgggcg
     2521 cacgtgtccc gccaccggcg ccgctacgcc acgggcgccg cggtagcgat gggcgcggcg
     2581 ggggccgcgc tgtggaagtc acgccgccgg ggcctcgtca cggcctgagt cgcgcactag
     2641 actgggcgcg tatcaaaggg cggtagctca gtttggtaga gcgctggtct ccaaaaccag
     2701 tggtcggggg ttcgagtccc tcccgccctg ctacctcttc gtgaacgtcg ccgtcaccg
//
LOCUS       DASQDO010000489         4972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_419573, whole genome shotgun sequence.
ACCESSION   DASQDO010000489 DASQDO010000000
VERSION     DASQDO010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419573"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VEV43_13465"
     CDS             <1..629
                     /locus_tag="VEV43_13465"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HYP24573.1"
                     /translation="PSFGAARREVAGVTGYAVLAHAGNVADLATFRIDTLVLAGWWGA
                     RELGIYAAAVNVAEVVLYLPSAVATVLLPHRSANEPVPPAVLVRTLGLVTAAAAGVAA
                     AGVAAAPWVVDLLYPAGFERAVGPLRVLFVAMVGTSVRRVLAAELAAVGRQGAASVVA
                     VVTLVAIVALDLLLIPEHGADGAAWASAAAYLLGGALIGVVVTRRRPL"
     gene            642..1805
                     /locus_tag="VEV43_13470"
     CDS             642..1805
                     /locus_tag="VEV43_13470"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP24574.1"
                     /translation="MQGPRVLLLITHLDPGGAQETVVLLADGLTRHGFDVTVAARPGA
                     EEARVELSGARVERLEHLYRPVAPLRDARAYRELRGLLREGFDVVHTHSSKAGVLGRL
                     AARRARVPAIVHTSHGLPVNPDMGRSARAVLLAAERAAAKTSDAIVAVSRATERELLE
                     LRLARPDQLVVIPSGVDVGRFADLGDRAAAKRALGVEPTAPVAGWIGRHFDQKRPADV
                     VRAALEITRAVPEAHFVMAGDGPLIDESRAAAAGERRVHVLGHRSEVGEVYAALDVML
                     LASAWEGLPRTVLEAHAAGVPVVSTDVSGVGEVVAHETTGFLAAPGDWRTLARRTVEL
                     LRDPERRDRMSRAARSRVDDFYSAAHTARATAELYRDVLSRKKAGPRRGGPAT"
     gene            complement(1759..2349)
                     /locus_tag="VEV43_13475"
     CDS             complement(1759..2349)
                     /locus_tag="VEV43_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYP24575.1"
                     /translation="MSSALVVSGDRLFGEAAAAALTEAGWDAAFVPDGLAAVASMARS
                     PVDALLVLGETVPRLGAVPLVQQARRRWPATTVVVVGNVPEVGAVVLPPDASRDEVVA
                     ALGAEPAAAVDEPARSRDGIAVLAGLTQRQRVVLRLLVEGLDMRAIGVELGLSEHTVR
                     THMQNLYARLGCHSRLELVRFATSQGLLAGDPPSSG"
     gene            complement(2346..3029)
                     /locus_tag="VEV43_13480"
     CDS             complement(2346..3029)
                     /locus_tag="VEV43_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP24576.1"
                     /translation="MPVSVLLVDPQPFFCESLAAALGPGVTVAGWTGDELAAAEMVER
                     HRPDVVLTELELAGGSGFGLVKRVGDGARVVVLTRRDEGDVLLDALEAGAHGVLGHAT
                     GLARLRDAVADAGAQSWIADPARLRASLLRARASHREASEGRVARLTVREREVLQLLA
                     RGLARDAIAASLYLSEHTVRTHLGNILKKLGVHSQAEAARVALRAGLGTPDVHVVRIS
                     GPDLGRPPA"
     gene            3161..4030
                     /locus_tag="VEV43_13485"
     CDS             3161..4030
                     /locus_tag="VEV43_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24577.1"
                     /translation="MEGLGFTVRSTDESFLQTLRGYLGAHRVGGHDGWVNLFSADCGE
                     SKTIAGGKVVHGISRLYSNGMKIFEGRVKDEMAGRLISGVRDLSTQHSNEFVRIRATG
                     VAFDGTAVLLPSPPQAHLPALAGLLVQRGGAFLGDEVVSIDPILRRAHGVPLPLLIDG
                     FDLPLFPGIEREPTRRRMRGTPEEIQAKTPRRPVPPEELGGVVAPPAPVGAIVFPFFE
                     EGAETRLEPFGGAPALFRFTEATLNLHVWTDRAFLLQRDLMQSVPVARLVVGSLPEAA
                     DVLTAAAPELVRG"
     gene            4171..4959
                     /locus_tag="VEV43_13490"
     CDS             4171..4959
                     /locus_tag="VEV43_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24578.1"
                     /translation="MDEGFAAALRWHLAPFRRETPETQAFPVDLYVAEKDEAVSPPIY
                     SLFVRNDLHYRSPDLTEITAHAVWDVHQLVPQSARDFLFLHAGAVARDGAALLLPARM
                     DSGKSSLVTALLRAGWGYLSDELGPLDPVTGRTYPFPKLISLDEASLAFFPGLADRLE
                     DRDASPLPERYVRVEDAGAAVAPPTPARWIVFPTPDWSGEARLEPLTSAQAVEKMADT
                     CFNLYRYGERGVVLLSRTAAAAQAFELRGGTPSERAALLTEELV"
BASE COUNT          647 a         1881 c         1787 g          657 t
ORIGIN      
        1 ctccgtcgtt cggagccgcg cgccgcgagg tcgccggcgt gacgggctac gcggtgctcg
       61 cgcacgcggg caacgtcgcg gacctcgcga cgttccggat cgacacgctc gtgctcgcag
      121 gatggtgggg tgcgcgcgag ctcggcatct acgcggcggc ggtcaacgtc gccgaggtcg
      181 tcctgtacct cccgtccgcg gtggcgaccg tgctgctgcc gcaccgctcg gcgaacgagc
      241 ccgtgccccc ggccgtgctc gtcaggacgc tcggcttggt caccgcggcc gcggccgggg
      301 tcgcggccgc cggggtcgcc gcggctccgt gggtcgtcga cctcctctat cccgccggct
      361 tcgagcgcgc cgtagggccg ttgcgcgtcc tgttcgtcgc catggtcggc acgagcgtcc
      421 ggcgcgtcct cgctgcggag ctcgcagcgg tcggccggca gggggccgcg tcggtggtcg
      481 cggtggtcac gctggtcgcg atcgtcgcgc tcgacctcct gctgatcccc gagcacggcg
      541 cggacggcgc ggcctgggcg tccgcggccg cgtacctgct cggcggggct ctgatcgggg
      601 tcgtcgtcac caggcggcgc ccgctctaga ctccgcggcc gatgcagggt ccccgcgtgc
      661 tgttgctgat cacccacctc gacccgggcg gcgcgcagga gaccgtggtg ctgctcgccg
      721 acggcctgac ccggcacggc ttcgacgtca ccgtcgcggc gcggccgggg gccgaagagg
      781 ctcgcgtgga gctgtcgggc gcccgcgtcg agcgcctcga gcacctctac cggccggtag
      841 caccgctccg cgacgcgcgc gcctaccggg agctccgcgg cctgctgcgc gagggcttcg
      901 acgtcgtgca cacgcactcg tccaaggccg gcgtgctcgg ccggctcgcg gcgcggcgcg
      961 cacgcgtccc cgcgatcgtc cacacgagcc acggcctgcc ggtcaacccc gacatgggcc
     1021 gcagtgcgcg ggcggtgctg ctcgcggccg agcgggccgc ggcgaagacg tccgacgcga
     1081 tcgtcgcggt gagccgcgcg accgaacgcg agctcctcga gctgaggctc gcgcggccgg
     1141 accagctcgt cgtgatcccg agcggcgtcg acgtcggccg gttcgcggac ctcggcgacc
     1201 gcgccgcagc gaagcgggcg ctcggggtcg agcccaccgc gcccgtcgcg ggatggatcg
     1261 ggcgccactt cgaccagaag cggcccgccg acgtcgtgag ggccgcgctc gagatcaccc
     1321 gcgccgtccc cgaggcgcac ttcgtcatgg ccggggacgg cccgctcatc gacgagtcgc
     1381 gtgccgccgc cgcgggagag cggcgcgtcc acgtcctcgg ccaccgctcg gaggtgggcg
     1441 aggtgtacgc ggccctcgac gtgatgctgc tcgcgtccgc gtgggagggg ctgccgcgca
     1501 ccgtgctcga ggcgcacgcg gcgggcgtgc cggtcgtgtc gaccgacgtc agcggcgtcg
     1561 gcgaggtcgt cgcccacgag acgacgggct tcctggcggc ccccggcgac tggcggacgc
     1621 tcgcgcggcg gaccgtcgag ctgctgcgcg accccgagcg ccgcgaccgg atgagccgcg
     1681 cggcccgctc acgcgtcgac gacttctact ccgcagcgca caccgcccgc gcgaccgcgg
     1741 agctctaccg agacgtcctc agccggaaga aggcgggtcc ccggcgagga ggccctgcga
     1801 cgtagcgaag cgcacgagct cgagcctgct gtgacacccg agccgcgcgt agaggttctg
     1861 catgtgcgtg cggacggtgt gctcgctgag cccgagctcc accccgatcg cgcgcatgtc
     1921 gaggccttcg acgagcagcc gcagcaccac gcgctgtctc tgcgtcaaac ccgcgaggac
     1981 ggcgatcccg tcacgcgacc gcgccggctc gtccaccgcg gccgccggct cggcccccag
     2041 cgcggccacg acttcgtccc tggacgcgtc cggtgggagg acgaccgcgc cgacctccgg
     2101 cacgttcccg acgacgacga cggtcgtggc cggccaccgt cgccgcgctt gctggacgag
     2161 cggaacggcc cccagccgcg ggaccgtctc gccgagcacg agcaacgcgt cgacgggtga
     2221 gcgcgccatg ctcgcgaccg cggcgaggcc gtcggggacg aacgccgcgt cccatccggc
     2281 ctcggtcagc gccgcggcgg cggcctcgcc gaacagccgg tcgcccgaga cgacgagggc
     2341 cgagctcacg ccggaggacg ccccaggtcg ggccccgaga tcctgacgac gtgcacgtcg
     2401 ggcgtgccga gccctgcgcg cagggccacc cgtgcggcct cggcctgcga atggacgccg
     2461 agcttcttca ggatgttgcc gaggtgcgtc ctgaccgtgt gctcgctgag gtagagcgac
     2521 gccgcgatcg cgtcgcgagc gagcccccgc gcgagcagct gcagcacctc gcgctcgcgc
     2581 accgtgaggc gtgcgacgcg gccctcggag gcctcacggt gcgacgcgcg cgcccgcagc
     2641 agggacgcgc gcaggcgggc cgggtcggcg atccacgact gcgcgccggc gtccgcgacc
     2701 gcgtcgcgca gccgcgccag tccggtcgcg tggccgagca cgccgtgcgc gccggcctcg
     2761 agagcgtcga gcagcacgtc gccctcgtcg cggcgggtga gcaccacgac gcgcgcgccg
     2821 tcgccgacgc gcttgacgag gccgaacccc gaccctccgg cgagctcgag ctcggtcagc
     2881 acgacgtcgg gacgatggcg ctcgaccatc tcggcggcgg cgagctcgtc gccggtccac
     2941 cccgcgacgg tcacgccggg ccccagcgcc gcggccagcg actcgcagaa gaacggctgg
     3001 ggatcgacca acaggaccga gaccggcacg ccgatatagt acgcgtccct tcccgcatcc
     3061 ccctttcgaa aggatcgacg tgccggacga gacgcgcact ctcacgccct gagaggagca
     3121 actcgagcgc ctaggttggg ccgccagcga tcactactcc gtggagggac tgggcttcac
     3181 cgtccggtcc accgacgaat ccttcctgca gacgttgcgc gggtacctcg gcgcgcaccg
     3241 cgtcggcggc cacgacggct gggtcaacct gttctccgcg gactgcggcg agtcgaagac
     3301 gatcgcgggc ggcaaggtcg tgcacggcat cagccgcctc tactcgaacg gcatgaagat
     3361 cttcgagggc cgcgtgaagg acgagatggc gggccggctg atctccggcg tcagggacct
     3421 ctcgacacag cactcgaacg agttcgtgcg catccgcgcg accggcgtgg ccttcgacgg
     3481 caccgcggtg ctgctcccgt ccccgccgca ggcacacctg ccggcgctcg ccggactgct
     3541 cgtgcaacgc ggtggagcgt tcctcggtga cgaggtcgtg agcatcgacc cgatcctgag
     3601 gcgcgctcac ggcgtgccgc tcccgctgct gatcgacggg ttcgacctcc cgctgttccc
     3661 ggggatcgaa cgcgagccga cgcggcgcag gatgcgcggg acgcccgagg agatccaggc
     3721 gaagacgccg cgacggcccg tcccgcccga agagctcggc ggcgtcgtcg ctccgcccgc
     3781 acccgtcggc gcgatcgtgt tcccgttctt cgaagagggg gccgagacga ggctcgagcc
     3841 gttcggcggc gccccggcgc tgttccgctt caccgaggcg accttgaacc tgcacgtctg
     3901 gacggaccgc gcgttcctgc tgcagcgcga cctcatgcag tccgtgccgg tcgcgcgcct
     3961 cgtcgtcgga tcgcttcccg aagcagccga cgtcctcacg gccgcggccc ccgaactggt
     4021 gagggggtga ccgcgatgcc cgacgaccac gaccacgagc acgaccacgg cgacgagcaa
     4081 cagtccggcg gctgaatcag cgacttcgac ccaggttggg tcgatcagga tcactaccgg
     4141 ctgctctcga tctcgttcac ggtccgctcc gtggacgagg gcttcgcggc cgcgctgcgg
     4201 tggcacctcg caccgttccg gcgcgagaca ccggagaccc aggccttccc cgtcgacctg
     4261 tacgtggccg agaaggacga agcggtatcg ccgccgatct actcgctgtt cgttcgcaac
     4321 gacctccact acaggtcgcc ggacctcacc gagataaccg cgcacgcggt gtgggacgtc
     4381 caccagctcg tgccgcagtc cgcccgcgac ttcctgttcc tgcacgccgg ggccgtggcg
     4441 cgggacggcg cagcactgct tctgcccgcg cggatggact cgggcaagtc ctcgctcgtg
     4501 accgcgttgc tgcgcgcggg atggggctac ctctccgacg agctcggccc cctcgacccc
     4561 gtgaccggca ggacctatcc gttcccgaag ctgatcagcc tcgacgaagc gtcccttgcg
     4621 ttcttcccgg ggctcgccga ccggctcgag gaccgcgacg cgtcgccgtt accggagcgg
     4681 tacgtccgcg tcgaggacgc gggagcggcc gttgcgccgc ccacgccggc gcgctggatc
     4741 gtgttcccga cccccgactg gagcggcgag gcccgcctcg agcccctcac aagcgcgcag
     4801 gccgtcgaga agatggccga cacctgcttc aacctctatc gctacggcga gcgcggcgtc
     4861 gtgctgctgt cgcgcacggc tgcggccgcg caggcgttcg agctgcgcgg cgggacgccg
     4921 tccgaacgcg ccgccctgct gaccgaggag ctcgtctagg agcggacgcg aa
//
LOCUS       DASQDO010000490         5322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_479934, whole genome shotgun sequence.
ACCESSION   DASQDO010000490 DASQDO010000000
VERSION     DASQDO010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479934"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VEV43_13495"
     CDS             complement(<1..737)
                     /locus_tag="VEV43_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYP24579.1"
                     /translation="MRRLLAAAAAAALVLTGCSIETVPDEGAATTPAPDVTLTQQART
                     APHSSLAQVVEEALPSVVNVRVESFDISGSSQEGQGSGVVIDKEGIILTNFHVVDAAT
                     EVTVSFQEKGREDLEGEVIGGVRGQDLAIIRVPADDLIPMEIGRSSDLKLGDDVTAIG
                     FPLGLGGPTVTSGIVSAIGRSITASNSGGGSEELQNLLQTDAAINPGNSGGPLIDAEG
                     RLVGINTAAVQAGAAENIGFAIPIDKAL"
     gene            complement(894..1271)
                     /locus_tag="VEV43_13500"
     CDS             complement(894..1271)
                     /locus_tag="VEV43_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24580.1"
                     /translation="MEAQISGTQRKVMTWSVRPAKLGDGASGIPVRGGKTLPFRVERS
                     WSAPAGVYPEQWFLVRPDSREVLHEGPVVERAIWGLQGLTEVAEDVTAPLALDPGSYL
                     VVFSLGGHMGGQAEIEAFEASGD"
     gene            complement(1355..1648)
                     /locus_tag="VEV43_13505"
     CDS             complement(1355..1648)
                     /locus_tag="VEV43_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24581.1"
                     /translation="MDEARLREDAEAHGSATVAGDLKTAGSYLDKEAYGPAGQVMEKM
                     PKPLEGSEVGSVEVEGDAALVQIRYSGGGDEVVIESRWAERDGTPKIVGLRVL"
     gene            complement(1662..2342)
                     /locus_tag="VEV43_13510"
     CDS             complement(1662..2342)
                     /locus_tag="VEV43_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HYP24582.1"
                     /translation="MFDIPKGRVVVFADIGCPWAHVAIHRLHETRARMGLGDRVVFDV
                     RAFPLEIVNRRPTPKKTLDAEIPVAGALAPDAGWQMWQRPEHEYPVTMLPALEAVHAA
                     KEQGPKIAEQLDRALRRGFFGDSRNVSMRHEILEIAESVDGLDTKALAQALDDGRARA
                     ALAEDRKLAESDEVDGSPHVFLPEGESVHNPGIEMHWEGEHGEGFPVVDKDDPTIYER
                     LLAAAARD"
     gene            2375..2791
                     /locus_tag="VEV43_13515"
     CDS             2375..2791
                     /locus_tag="VEV43_13515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HYP24583.1"
                     /translation="MCVFCEIVRGEGAAHVVFEDEVSLAFLDVRPVFEGHTLLVPREH
                     HETLADLPPALIEPLFANVQMLSRAVVSAVDAKGSFVAMNNKVSQSVPHLHVHVVPRR
                     PKDGLRGFFWPRRKYRDDDDMAAVAESIRAAVGALA"
     gene            2788..3438
                     /locus_tag="VEV43_13520"
     CDS             2788..3438
                     /locus_tag="VEV43_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP24584.1"
                     /translation="MTQDLTIRSRDGLALEASVDTPDSPKAVLVFCHPHPQMGGTMNA
                     PLLLAVRDEMLSRGWAVLRFNFRGIGASEGVSGTGVDEVADAGGAADHAAKEFEGLPR
                     ALAGWSFGGAVAVRLAATDPSFVGCAAIAPAVDEKPGVTAGLPPPGRLAVEVPLLVVY
                     GANDKQVSPDACSSWGEAAGADVVRVSGANHFFWAKYDQLSKIVGDWLDARLEAHR"
     gene            3435..4265
                     /locus_tag="VEV43_13525"
     CDS             3435..4265
                     /locus_tag="VEV43_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYP24585.1"
                     /translation="MKIGIDCSQHQLTWDALLDRVRFAEEIGADSAWVFDHFKAMYGP
                     PGGPCLESWTLMSALGAATSRIRLGALVTGVTYRHPSMLAMEAVTVDHVSNGRLEFSL
                     GAAWFEPEHRELGFDFPPPGERGRRLEEALQVVKLLMTEDGATFDGRYYRLEDASYYP
                     RPVQQPHPPIWVGAGGEQVMLPIAGRHADVWHGFGHVSELDRKWRIVAEHAEKAGRDP
                     ATITRSTSLSISESWDEIKATAAALRDAGIGYVIVSWPGEGKARVEELWEKVVPELTG
                     "
     gene            complement(4262..5023)
                     /locus_tag="VEV43_13530"
     CDS             complement(4262..5023)
                     /locus_tag="VEV43_13530"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HYP24586.1"
                     /translation="MRRIVTALIAALVAAAVQSAGASVPAESARLGDREAPCPGATLL
                     PTTDFLETFPDTIVDLDVLVVTDPADLAYAREAMKVAALAYEPYSIRLVPTYETIPLP
                     KIGNGGTRLLKWLKRQFGGARPPGSDVVYLATNRLVRAAGRADCVGGIAGDSTAFAVG
                     TLRWDKIVGVELRGVPDPRLPNADGGAKIAAHEIGHLLGAEHDDSVCGPGMRPTDPAR
                     PCEVMLTKSPQNVGLHFGPLNGAVVRRFALAYARP"
     gene            complement(5036..>5322)
                     /locus_tag="VEV43_13535"
     CDS             complement(5036..>5322)
                     /locus_tag="VEV43_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HYP24587.1"
                     /translation="GLDELLSWARAQDLIVVATSARGRVDHWAAEYPERCFLLFGSEA
                     TGLTDETLAAADEVVRIPQQGAASSLNLAVAAGIVVYEARRPRPGSPGPA"
BASE COUNT          740 a         1854 c         1925 g          803 t
ORIGIN      
        1 agcgccttgt cgatcgggat cgcgaagccg atgttctccg cggcccccgc ctggaccgca
       61 gcggtgttga tcccgacgag ccgtccctcc gcgtcgatca gcggcccgcc cgagttcccg
      121 gggttgatcg cggcgtccgt ctgcagcagg ttctggagct cctcggaccc accgccggag
      181 ttgctcgcgg tgatgctcct gccgatggcg gacacgatgc cggaggtgac ggtcggcccg
      241 ccgaggccga gcgggaaccc gatcgcggtg acgtcgtcgc cgagcttcag gtccgaggac
      301 cggccgatct ccatcgggat caggtcgtcg gcggggaccc ggatgatcgc aaggtcctgc
      361 cctcgcacgc ccccgatgac ctcgccctcg aggtcctccc ggcccttctc ctggaacgac
      421 accgtgacct cggtcgcagc gtcgaccacg tggaagttcg tgaggatgat cccctccttg
      481 tcgatgacga cgccggagcc ctggccctcc tggctgctcc cggagatgtc gaacgactcg
      541 acccgcacgt tgacgaccga gggaagggcc tcctcgacga cctgcgccag agaggaatgg
      601 ggcgccgtcc gcgcctgctg cgtcagcgtg acgtcggggg cgggtgttgt cgcggccccc
      661 tcgtcgggga cggtctcgat cgaacagccg gtaaggacga gggccgcggc ggccgcggcg
      721 gcgagaaggc gtctcatcgc accgaggata gggcggcccc cgggaaaagt gagcgctcgc
      781 ggagaggccg gctgctgtgg acgccgtaag gggaagctcc ggcgcgcctc agtaaccggc
      841 cgttttccag tttgaacgac cccccgacgg gggtcaaggt ccgggaagag cgctcagtcg
      901 ccggaggctt cgaacgcctc gatctcggcc tggcccccca tgtgcccgcc cagcgagaac
      961 acgacgaggt aggagccggg gtcgagggcc agcggagcgg tgacgtcctc ggcgacctcg
     1021 gtgaggccct gaaggcccca gatcgcgcgc tcgacgacag gcccctcgtg caagacctcg
     1081 cggctgtccg ggcgcacgag gaaccactgc tcggggtaca cgccggcggg agcggaccac
     1141 gagcgctcga cgcggaacgg aagcgtcttg ccgccgcgca ccgggatgcc ggaggcgccg
     1201 tcgccgagct tcgccggacg gaccgaccac gtcatgacct tgcgctgggt gccgctgatc
     1261 tgggcttcca tcgcccggaa agataccccg ccttggcggt ctcgccgagg cgaccccgtc
     1321 gccctagcgg tctcgccgag gcgaccccgc ggtcctagag gacgcggagc ccgactatct
     1381 tcggcgtccc gtcgcgctcg gcccagcggg actcgatcac gacctcgtcc ccgccgcccg
     1441 agtacctgat ctgcacgaga gccgcgtcgc cctcgacctc gacggagccg acctcggagc
     1501 cctcgagcgg cttcggcatc ttctccatga cctgcccggc ggggccgtag gcctccttgt
     1561 cgaggtaact gccggcggtc ttgagatcgc ccgccaccgt cgcgctgccg tgggcctccg
     1621 cgtcctccct gagccttgcc tcgtccatcg tcggtctcct ctcagtctcg cgctgccgcc
     1681 gcgagcaggc gctcgtagat ggtggggtcg tctttgtcta ctacggggaa accctcgccg
     1741 tgctcgccct cccagtgcat ctcgatgccg gggttgtgca cgctctcccc ctccgggagg
     1801 aagacgtggg ggctgccgtc gacctcgtcg gactcggcga gcttgcggtc ctcggcgagg
     1861 gccgcccggg cgcgcccgtc gtcgagggct tgcgcaagcg ccttcgtgtc gagaccgtca
     1921 accgactccg ctatctccag gatctcgtgg cgcatcgaga cgttgcggct gtcgccgaag
     1981 aagccgcgtc ggagcgcccg gtcgagctgc tccgcgatct tcgggccctg ctccttcgcc
     2041 gcgtggaccg cttcgagggc cgggagcatc gtgacgggat actcgtgttc cggccgctgc
     2101 cacatctgcc accccgcgtc gggcgcgagc gctcccgcga cggggatctc ggcgtcgagc
     2161 gtcttcttgg gagtcggccg gcggttgacg atctcgagcg ggaacgcgcg gacgtcgaag
     2221 acgacgcggt cgccgaggcc catgcgggcg cgcgtctcgt ggagccggtg gatcgcgacg
     2281 tgcgcccagg gacacccgat gtcggcgaac acgacgacgc ggcccttggg gatgtcgaac
     2341 atgccgggga acgtaccccg catagggtga cgggatgtgc gtgttctgcg agatcgtgcg
     2401 cggtgaaggg gccgcgcacg ttgtgttcga ggacgaggtc agcctcgcgt tcctcgacgt
     2461 gcggccggtc ttcgagggcc acaccttgct cgtcccgagg gagcaccacg agacgctcgc
     2521 ggacctgccc cccgcgctga tcgagcccct gttcgcgaac gtccagatgc tgtcgcgcgc
     2581 ggtcgtctcc gcggtcgacg cgaaggggtc gttcgtcgcg atgaacaaca aggtgagcca
     2641 gagcgtcccg cacctccacg tgcacgtcgt gccgcggcgg ccgaaggacg ggctgcgcgg
     2701 cttcttctgg ccccgccgca agtaccggga cgacgacgac atggccgccg tcgccgagtc
     2761 gatccgcgcc gcggtcggcg cgctcgcatg acgcaagacc tcacgatccg ctcgcgcgac
     2821 gggctcgcgc tggaggcctc ggtcgacacg cccgactcgc cgaaggccgt cctcgtgttc
     2881 tgccacccgc accctcagat gggcgggacg atgaacgctc ccctgctgct ggcggtgcgg
     2941 gacgagatgc tgtcgcgggg ctgggccgtg ctgcggttca acttccgcgg catcggggcg
     3001 tccgaggggg tctcggggac cggggtcgac gaggtcgccg acgccggggg ggccgccgac
     3061 cacgcggcga aggagttcga ggggctgccg cgcgcactcg ccggatggtc gttcgggggg
     3121 gccgtcgcgg tgcgcctcgc ggccacagat ccttcgttcg tcggctgcgc ggcgatcgct
     3181 ccggcggtcg acgagaagcc gggggtgacc gcgggcctgc cgccgccggg acggctcgcg
     3241 gtcgaggtgc cgctgctcgt cgtctacgga gcgaacgaca agcaggtgtc gccggacgcg
     3301 tgctcgtcgt ggggcgaggc ggccggggcc gacgtcgtgc gtgtctccgg ggcgaaccac
     3361 ttcttctggg cgaagtacga ccagctgtcc aagatcgtcg gcgactggct cgacgccaga
     3421 ctggaggcgc accggtgaag atcgggatcg actgctcgca acatcagctg acgtgggacg
     3481 cgctgctcga ccgggtgcgg ttcgcggagg agatcggcgc ggactccgct tgggtcttcg
     3541 accatttcaa ggcgatgtac ggcccccccg gcgggccctg tctcgagagc tggacgctca
     3601 tgtccgcgct cggtgccgcg acgtcgagga tccggctcgg cgcgctcgtc accggcgtca
     3661 cctaccggca tccgtcgatg ctcgcgatgg aggccgtgac cgtcgaccac gtctcgaacg
     3721 gccggctcga gttctcgctc ggggccgcgt ggttcgagcc cgagcatcgc gagctcggct
     3781 tcgacttccc gcctcccggt gagcgcggcc gccgtctcga ggaggcgctg caggtcgtga
     3841 agctgctgat gaccgaggac ggcgccacct tcgacggccg ctactaccgg ctcgaggacg
     3901 cgtcgtatta cccgaggccc gtgcagcagc cgcacccgcc gatatgggtg ggcgcggggg
     3961 gcgagcaggt gatgctcccg atcgcgggac ggcacgcgga cgtgtggcac ggcttcggcc
     4021 acgtcagcga gctcgaccgc aagtggagga tcgtcgcgga gcacgcggag aaggcgggcc
     4081 gagacccggc gacgatcacg cgctcgacga gtctttcgat ctcggagtcg tgggacgaga
     4141 tcaaggcgac ggccgcggcc ctgcgcgacg ccgggatcgg ctacgtgatc gtgagctggc
     4201 cgggcgaagg caaggcccgg gtcgaggagc tctgggagaa ggtcgtcccg gagctgaccg
     4261 gctagggccg cgcgtaggcc agcgcgaacc tgcgtacgac cgctccgttc aacgggccga
     4321 agtgcaggcc gacgttctgg ggcgacttcg tcaacatgac ctcgcagggg cgcgccgggt
     4381 cggtcggccg cattccgggc ccgcataccg agtcgtcgtg ctcggccccc aggagatgac
     4441 cgatctcgtg agcggcgatc ttcgccccgc cgtcggcatt ggggagccgc ggatcgggga
     4501 cacctcggag ctccacaccg acgatcttgt cccaccgcag cgttccgacc gcgaacgcag
     4561 tcgagtcgcc ggcgatgcct ccgacgcagt cggcgcggcc ggccgccctg accagccggt
     4621 tcgtcgccag gtacacgacg tcggagcccg ggggccgcgc gccgccgaac tgtcgcttca
     4681 gccacttcag cagcctcgtg ccgccgttgc cgatcttggg caacgggatg gtctcgtacg
     4741 tcgggaccag acggatcgag tacggctcgt acgcgagcgc ggcgaccttc atcgcttctc
     4801 tggcgtaggc gaggtcggcg ggatccgtca cgacgagcac gtcgaggtcg acgatcgtgt
     4861 cggggaaggt ctcgaggaag tcggtggtgg gaaggagcgt ggcgccgggg cacggggcct
     4921 cgcgatcccc gaggcgtgcc gactcggccg gaacggacgc gccggcggat tgcacggcgg
     4981 ccgcgacgag cgcggcgatc aacgcagtga cgatcctcct cacgcgggac gtcttctacg
     5041 cggggccggg cgatcctggg cgcggccgtc tcgcttcgta gaccactatc cccgccgcga
     5101 ccgcgaggtt cagcgacgac gcggcgccct gctgcgggat ccgcaccacc tcgtcggccg
     5161 ccgccagcgt ctcgtcggtc aagcccgtcg cctcgctacc gaacaggaga aagcaccgct
     5221 cggggtactc cgccgcccag tggtcgaccc ggccgcgggc cgaggtggcg acgacgatca
     5281 ggtcctgcgc tctggcccac gacaggagct cgtcgagccc cg
//
LOCUS       DASQDO010000491         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_113347, whole genome shotgun sequence.
ACCESSION   DASQDO010000491 DASQDO010000000
VERSION     DASQDO010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113347"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..297
                     /locus_tag="VEV43_13540"
     CDS             148..297
                     /locus_tag="VEV43_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24588.1"
                     /translation="MSDDDKTLGREVVAHAAEVHPEDVRTPEEVAAHVAAEAEDAPDR
                     PPWAY"
     gene            complement(306..638)
                     /locus_tag="VEV43_13545"
     CDS             complement(306..638)
                     /locus_tag="VEV43_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24589.1"
                     /translation="MDGPARDRLAKNEALFRDVNERVKRIDAAQGVPEDELWDFLCEC
                     GDGGCLARVSLTIVEYERVRANSVQFFVVPGHEEPEVERVVEDTRRYAVVEKKPGERK
                     IAIERDPR"
     gene            complement(644..979)
                     /locus_tag="VEV43_13550"
     CDS             complement(644..979)
                     /locus_tag="VEV43_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24590.1"
                     /translation="MAGRQDRQERRALTQVMFRAANEAILGPARGRDPLTFICECGDE
                     MCFDVIELTLEEYESVRADGRCFAIASGHEGDGDSVLTEVERFTLVEKVGAGMRIATD
                     RDPRALMRD"
     gene            complement(1023..>3293)
                     /locus_tag="VEV43_13555"
     CDS             complement(1023..>3293)
                     /locus_tag="VEV43_13555"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HYP24591.1"
                     /translation="TYTRGIEAERQVPLLTRLSEVEGFERYLRRAFLGQKQFSIEGLD
                     VMVPMLDEAIELATASGAKEIVLGMAHRGRLNVVAHTVGRPYDVILKEFEGERLLEAV
                     THDPEGGTGDVKYHLGSEGIRKTQSGDVHVRLVTNPSHLEAVDPVVEGTTRALQTDHE
                     SPEGSHDPKSAFAIQIHGDAAFPGQGIVAETLNLGALEGYSTGGTLHLIANNQIGFTT
                     NPREGRSTHYSSDLAKGYDIPIIHVNADDAEAAVGAIRLAMAYRDEFDMDIVLDLVGY
                     RRFGHNEGDEPAYTQPLMYEKIANHPTVRELYAKELAERGVVTPEQAEELVTAVEARM
                     RDAHDTLKATISAAGGSAVHAERRPGRARGEEPETAVPAAELERLNRDLLQVPDGFTV
                     HPKLLKQLERRVNAFGAEGGSDWALAEALAFGMLLREGVPVRLTGQDTERGTFAHRHI
                     VLHDAHTDATWAPIQHLPDARAPFQVYNSPLSEAGCVGFEYGYAVARPDALVLWEAQF
                     GDFVNGAQVIVDQFLVASLAKWHETSRLTLLLPHGYEGNGPEHSSARVERFMQMAAQE
                     NMRIVNCTTAAQYFHVLRRQAHHPKPRPLVVLTPKSLLRLKQATSKLEHLTDSYFRPL
                     IDDLERDAEARRRTERLILCTGKVYYDIVGHEARSESDDVAVARLEQRYPVPVAAYDE
                     LLSRYPNVSEVVWTQEEPQNMGAWRAIRHRLEEGLPAEVELRYCGRPWRASPSEGYPT
                     AHLVEQDRIVTEALSA"
BASE COUNT          439 a         1156 c         1162 g          536 t
ORIGIN      
        1 cgagtccgtc gagctcgacc gccgccgcga ggagttcgac cggcagcggc tggagatctg
       61 aaaatgccgc gtccggctag ggcacacgag ctcccgcggg gtcggcgcta ggtgcgggcc
      121 gtccgctgct ggtgcgggga gctcgtcgtg agcgacgacg acaagacgct cgggcgggag
      181 gtcgtcgcgc acgccgccga ggtccacccg gaggacgtcc gcactcccga agaggtggct
      241 gcccacgtcg ccgccgaggc cgaggacgct ccggaccggc ctccgtgggc gtactagcgt
      301 cggcgttacc gcgggtcgcg ctcgatcgcg atcttccgct cgccgggctt cttctccacc
      361 acggcgtacc gccgcgtgtc ctcgacgacc cgttccacct ccggctcctc gtggccgggc
      421 acgacgaaga actgcacgga gttcgcccgg acgcgctcgt actcgacgat ggtcagcgag
      481 acgcgcgcga gacagccgcc gtcgccgcac tcgcagagga agtcccagag ctcatcctcg
      541 ggcacaccct gcgcggcgtc gatccgcttg acgcgctcgt tcacgtcgcg gaagagggcc
      601 tcgttcttcg ccaggcggtc gcgggcgggc ccgtccatcg gggctagtct cgcatgagcg
      661 cgcgcgggtc gcgatccgtc gcgatgcgca tcccggctcc caccttctcg acgagagtga
      721 accgctcgac ctccgtgagg acggagtcgc cgtcaccctc gtgaccggac gcgatcgcga
      781 agcagcggcc gtcggcgcga acgctctcgt actcctcgag cgtgagttcg atcacgtcga
      841 agcacatctc gtccccgcac tcgcagatga acgtcagcgg gtcgcgccct ctcgccggcc
      901 cgaggatcgc ctcgttcgcc gcacggaaca tcacctgggt cagcgcacga cgttcctggc
      961 gatcctggcg cccggccacc acaagcggct atccggctcg cggcagccga aaacacccgg
     1021 aactacgcgc tcagggcctc ggtgacgatc cgatcctgct cgacgaggtg tgccgtcggg
     1081 tagccctcgc ttgggctcgc ccgccacggg cggccgcagt agcgcagctc gacctcggcc
     1141 ggcagaccct cctcgagccg gtgccggatc gcgcgccagg cgcccatgtt ctgcggctcc
     1201 tcctgcgtcc agacgacctc gctcacgttc gggtagcgcg acaggagctc gtcgtaggcc
     1261 gcgaccggga ccgggtagcg ctgctcgagt cgcgcgaccg cgacgtcgtc gctctcgctg
     1321 cgcgcctcgt gcccgacgat gtcgtagtag accttgccgg tgcagaggat gaggcgctcg
     1381 gtgcgccgcc gcgcctcggc atctcgctcg aggtcgtcga tcagagggcg gaagtagctg
     1441 tccgtgaggt gctcgagctt cgacgtggcc tgcttcaggc gcaggaggct cttgggggtg
     1501 aggacgacga gcggacgcgg cttcggatgg tgggcctggc ggcggagcac gtggaagtac
     1561 tgcgcggccg tggtgcagtt gacgatccgc atgttctcct gcgcggccat ctgcatgaag
     1621 cgctcgacgc gggcgctcga gtgctccggc ccgttcccct cgtagccgtg cgggaggaga
     1681 agcgtcaggc gcgaggtctc gtgccacttg gcgaggctcg cgacgaggaa ctggtcgacg
     1741 atcacctgcg ctccgttgac gaagtctccg aactgggcct cccagaggac gagcgcgtcg
     1801 ggccgcgcga ccgcgtagcc gtactcgaac cccacgcagc cagcctcgga gagcggcgag
     1861 ttgtagacct ggaatggcgc ccgcgcatcc gggaggtgct ggatgggagc ccaggtcgcg
     1921 tccgtgtgcg cgtcgtggag gacgatgtga cggtgcgcga aggtgccgcg ctccgtgtcc
     1981 tggccggtca ggcgcacggg gacgccctcg cggaggagca tcccgaacgc gagcgcctcc
     2041 gccagcgccc agtcgctgcc gccctcggcg ccgaacgcgt tcacacgccg ctcgagctgc
     2101 ttcaggagct tcgggtggac ggtgaagccg tccggcacct ggaggaggtc gcggttcagc
     2161 cgctcgagct cggccgccgg gacggccgtc tccggctcct cgcctcgggc acgccccggc
     2221 ctccgctccg cgtggacggc gctgcccccc gcggccgaga tcgtcgcctt cagcgtgtcg
     2281 tgggcgtccc gcatgcgcgc ctcgacggcc gtcacgagct cctcagcctg ctcgggcgtg
     2341 accacgccgc gctcggcgag ctccttcgcg tagagctcgc gcacggtcgg gtggttcgcg
     2401 atcttctcgt acatgagcgg ctgcgtgtag gcgggctcgt cgccttcgtt gtggccgaag
     2461 cgccggtagc cgacgaggtc gaggacgatg tccatgtcga actcgtcccg gtaggccatc
     2521 gccagccgaa tcgcgccgac cgcggcctcc gcgtcgtcgg cgttcacgtg gatgatcggg
     2581 atgtcgtagc ccttggcgag gtcgctcgag tagtgcgtcg agcgcccttc gcgcggattc
     2641 gtcgtgaagc cgatctggtt gttcgcgatc aggtggaggg tgccgcccgt cgagtagccc
     2701 tcgagcgcgc cgaggttcaa cgtctcggcg acgatcccct ggccggggaa ggccgcgtcg
     2761 ccgtggatct ggatcgcgaa ggccgacttc ggatcgtggc tgccctctgg gctctcgtgg
     2821 tcggtctgaa gggcgcgcgt cgttccctcg acgaccgggt cgaccgcctc caggtggctc
     2881 gggttggtga cgaggcggac gtggacgtca ccgctctgcg tcttgcggat cccctcggag
     2941 ccgaggtggt acttgacgtc gcccgttccg ccctcggggt cgtgcgtcac ggcctcgagc
     3001 agccgctcgc cctcgaactc cttcaagatc acgtcgtagg gtcggccgac cgtgtgcgcg
     3061 acgacgttca ggcggccgcg gtgcgccatg ccgagcacga tctccttcgc gccgctcgcg
     3121 gtcgcgagct cgatcgcctc gtccagcatc ggcaccatca cgtccaggcc ctcgatcgag
     3181 aactgcttct ggccgaggaa ggctctgcgc aggtagcgct cgaagccctc gacctcggag
     3241 agccgggtca ggagcgggac ctgccgctct gcttcgatcc cgcgcgtgta ggt
//
LOCUS       DASQDO010000492         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_786732, whole genome shotgun sequence.
ACCESSION   DASQDO010000492 DASQDO010000000
VERSION     DASQDO010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786732"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /gene="cofG"
                     /locus_tag="VEV43_13560"
     CDS             complement(<1..1386)
                     /gene="cofG"
                     /locus_tag="VEV43_13560"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HYP24592.1"
                     /translation="MSRLDELAGRASGGERLDGADARALASLAPSDPATVLAAAAAKR
                     DAATGPVVTFSKKVFIPLTKLCRDACGYCTFAHPPRPGEAAYLTLDEVLAIARAGRDA
                     GCAEALFTLGDKPERKWREARAELDALGHDSTVSYLVAACAAVLDETGLLPHANPGAL
                     TPEETAALRAVTVSQGTMLETLSERLLGRGMAHFGAPDKKPSVRLATLEAAGAARVPF
                     TTGILVGIGETPDERVDALLAIRHSHERHGHVQEVIVQSFRAKPDTLMARHPEPALDE
                     MLLTVALARLLLPDDVAVQAPPNLTEDYGAYLDAGVNDWGGVSPVTPDHVNPEKPWPH
                     LEELARTTAARGFLLTERLAAYPRYCAEPAALAAWVDPALHARVLDEIDGDGLRRSGE
                     WFSGGDAPPPEGDAAAVEAAGVGAWRVPGRVVRSRVASALDRAEAGDELDEAEVALLF
                     TARGAEAERLYA"
     gene            1414..2337
                     /gene="cofD"
                     /locus_tag="VEV43_13565"
     CDS             1414..2337
                     /gene="cofD"
                     /locus_tag="VEV43_13565"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HYP24593.1"
                     /translation="MNVTAICGGVGAAKLLIGISRALAGQGVTAVVNTADDDEIYGVH
                     VSPDLDTITYWLSGLADTERGWGLAQDTFNLVEGLRDLGAEAWFNLGDRDFATCLLRK
                     SMLDSGSSLSEVTAQVADALGVVHRLLPMTDDRVRTRVTAAGGRTLTFQEYFVRERCE
                     PEVTGVTFEGVEDAAPAPGVLDAIATADVVVVCPSNPVVSIGPVLAVPGIADALARHP
                     FVVAVSPIVGGRAIKGPADRMLESLGHGSTAAAVARGYGGFCDHFVVDVRDAGEVETV
                     QSLGMACTALDTMMVDGTTSEKLARDLLALR"
     gene            2334..>2676
                     /locus_tag="VEV43_13570"
     CDS             2334..>2676
                     /locus_tag="VEV43_13570"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HYP24594.1"
                     /translation="MNEIRVVPVTGVPEVRRGDDLGALLVTATEGLPLEDGDVVVVTQ
                     KVVSKAEGRVVPGDERDRALAVETKRVLRRSGGMVIAETAHGFVCANAGIDSSNVPGD
                     DVVLLPLDPDAS"
BASE COUNT          352 a          963 c          985 g          376 t
ORIGIN      
        1 ggcgtagaga cgttccgcct cggccccgcg ggccgtgaac agcagcgcga cctcggcttc
       61 gtcgagctcg tcgccggcct cggcgcggtc gagcgcggag gcgacgcgcg aacgcacgac
      121 gcggcccggc acccgccacg ctccgacgcc cgcggcctcg actgcggccg cgtctccctc
      181 gggcggcggt gcgtcgcccc ccgagaacca ctcccccgac cgccgcaacc cgtcgccgtc
      241 gatctcgtcg agcacccggg catgcagcgc gggatcgacc cacgccgcga gggccgccgg
      301 ctcggcgcag tagcgcgggt aggccgccag gcgttccgtg agcaggaagc cgcgcgcggc
      361 ggtcgtccgg gcgagctcct cgaggtgcgg ccacggcttc tcggggttga cgtggtcggg
      421 agtgaccggc gagaccccgc cccagtcgtt cacgcccgcg tccaggtagg cgccgtagtc
      481 ctcggtcagg ttcggcggcg cctgcacggc gacgtcgtcc gggagcagca gccgcgcgag
      541 cgcgaccgtc agcagcatct cgtccagagc gggctcggga tgacgcgcca tcagcgtgtc
      601 cggcttggcg cggaagctct ggacgatcac ctcttggacg tggccgtggc gttcgtgcga
      661 gtgcctgatc gcgagcaggg cgtcgacgcg ctcgtccggc gtctcgccga tgccgacgag
      721 gatcccggtc gtgaacggga cgcgtgcagc tcccgcggcc tccagcgtcg cgagccgcac
      781 cgacggcttc ttgtcggggg cgccgaagtg cgccatgccg cgcccgagca gccgctcgga
      841 gagcgtctcg agcatcgtcc cctggctgac cgtcaccgca cggagagcgg ccgtctcctc
      901 cggcgtcagc gcgccggggt tcgcgtgggg cagcagcccg gtctcgtcga gcaccgcggc
      961 gcacgcggcg acgagatagg agacggtcga gtcgtggccc agcgcgtcga gctcggcgcg
     1021 ggcctcgcgc cacttgcgct ccggcttgtc gccgagggtg aacagcgcct cggcgcagcc
     1081 cgcgtcgcgc cccgcgcgcg cgatcgccag cacctcgtcg agcgtgagat acgcggcctc
     1141 gccgggacgc ggcgggtgcg cgaacgtgca gtatccacac gcgtcgcggc acagcttcgt
     1201 gagcgggatg aagaccttct tcgagaaggt gacgaccggg ccggtggccg cgtcccgctt
     1261 ggccgcagcc gccgcgagga ccgtcgccgg atcggacgga gcgaggctcg caagggccct
     1321 cgcatcggcc ccgtccagcc tttcgccgcc ggacgcgcgc cccgcgagct cgtccagtcg
     1381 gctcatcgca cgaggctact agactgcacc ggcgtgaacg tcactgcgat ctgtggcgga
     1441 gtcggcgcgg cgaagctgct catagggatc agccgcgctc tcgccggcca aggcgtgacc
     1501 gccgtcgtca acaccgcgga cgacgacgag atctacggcg tccacgtctc gcccgatctc
     1561 gacacgatca cgtactggct gtcgggcctc gccgataccg agcgcggctg gggccttgct
     1621 caggacacgt tcaacctcgt cgagggcttg cgcgacctgg gggccgaggc gtggttcaac
     1681 ctcggcgacc gcgacttcgc cacgtgcctg ctgcggaagt cgatgctcga ctcgggctcg
     1741 tcgctgagcg aggtgacggc tcaggtcgcc gacgcgctcg gcgtggtcca caggctgctg
     1801 ccgatgaccg acgaccgcgt gaggacgcgc gtcactgccg ccggcggccg gacgctgacg
     1861 ttccaggagt acttcgtgcg cgaacggtgc gagcccgagg tcaccggcgt gacgttcgag
     1921 ggggtcgagg acgcggcccc cgcaccgggg gtcctggatg cgatcgccac cgcggacgtc
     1981 gtcgtcgtgt gcccgtcgaa ccccgtcgtc tcgatcggtc ccgtcctcgc cgtccccggc
     2041 atcgcggacg cgctcgcgcg ccacccgttc gtagtcgcgg tctcaccgat agtcgggggc
     2101 cgggcgatca aggggcccgc cgatcgcatg ctcgagtccc tcggtcacgg ctcgacagcc
     2161 gccgcggtgg cgcgggggta cggagggttc tgcgaccact tcgtcgtcga cgtgcgcgac
     2221 gcgggcgagg tcgagacggt gcagtctctc gggatggctt gcacggcgct cgacacgatg
     2281 atggtcgacg ggacgacgtc ggagaagctc gcacgcgacc tcctggcgct tcgttgaacg
     2341 agatcagggt cgtcccggtg acgggcgttc ccgaggtccg ccgcggagac gacctcggcg
     2401 cgctgctcgt gacggcgacg gagggtcttc ccctcgagga cggcgacgtc gtggtcgtca
     2461 cgcagaaggt ggtctcgaag gccgagggcc gcgtggtgcc cggcgacgag cgcgaccggg
     2521 cgctggccgt cgagacgaaa cgcgtcctgc ggaggtccgg gggcatggtg atcgccgaga
     2581 ccgcgcacgg cttcgtctgc gcgaacgccg gcatcgactc ctcgaacgtc ccgggcgacg
     2641 acgtcgtgct cctgcccctc gaccccgacg cgtcgg
//
LOCUS       DASQDO010000493         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_602903, whole genome shotgun sequence.
ACCESSION   DASQDO010000493 DASQDO010000000
VERSION     DASQDO010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602903"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VEV43_13575"
     CDS             complement(<1..351)
                     /locus_tag="VEV43_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24595.1"
                     /translation="MSVQIIQAIVNAKVVVADLTGRNANVYYELGVAHSFAKPLLILV
                     DSTDSLAFDLQGERAIVIGDDGAEIGARKAAAAADEVRRQLDVVLAPGYVPQSLVRGA
                     GVTSSLERFATDDPV"
     gene            complement(581..754)
                     /locus_tag="VEV43_13580"
     CDS             complement(581..754)
                     /locus_tag="VEV43_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HYP24596.1"
                     /translation="MALEMRGSCERCGTGLAPNDEAFICSYECTFCSACAREMASVCP
                     NCGGELVARARRV"
     gene            complement(759..1676)
                     /locus_tag="VEV43_13585"
     CDS             complement(759..1676)
                     /locus_tag="VEV43_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24597.1"
                     /translation="MRLEGIHHITAITGDAPGNVEFYAGTLGLRMVKKTVNQDDPTVY
                     HLFYADEKGSPGSDITFFEYPGLSRGRAGAGMVHRIVWRVASPESLDFWAGRLAEAGV
                     EVERGEGYLRFEDPEGLGLELRVTDSSEPPLRASHPDVPAEHALLGFDGVRAYSADAS
                     ESAEFLRDGLGFTESDPGQWTARGEERGSFYGYDPAPEARGLNGAGTVHHVAWSSPID
                     EHDAWRERVAGAGAQVTPVIDRFYFKSIYFREPSGILFEIATMGPGFTADEPLESLGE
                     SLSLPPNYQHLREKLEEVLTPLPNPRGRA"
     gene            1808..2464
                     /locus_tag="VEV43_13590"
     CDS             1808..2464
                     /locus_tag="VEV43_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24598.1"
                     /translation="MRKVLAAVLVCLATLGGPAAHAAQQLSADDPPTAEGNFYEPDQQ
                     CDDQTLKSNGKPVIKVAFCIYFFAFDPLWELDLNNDYGVAWAQATFDALPGYCTSELS
                     FYIEIPEGGGYQMHARVPGETITTRSTTSTLVHLGATAAENAIQPGSVSQALELLPGK
                     LTPTGKAGEPRVGVSWKGRTGAKLAFATGAEVSWNWLSAPPEIRLGADTIKLTSGKGC
                     "
     gene            complement(2471..2545)
                     /locus_tag="VEV43_13595"
     tRNA            complement(2471..2545)
                     /locus_tag="VEV43_13595"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2508..2510),aa:Asp,seq:gtc)
     gene            2641..>3284
                     /locus_tag="VEV43_13600"
     CDS             2641..>3284
                     /locus_tag="VEV43_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24599.1"
                     /translation="MGPTMRHLSALAAAVVVAASLSPSAAAAPRTRSIELTDCSEHTA
                     FFPIPRAQGRDYVPAGFDPAAAPADNDNLTNFYVTSWVCGDPENPALELALTHLVVDP
                     PRDASASNRYLVDVVAEGPAAASLRKVTCVPAEAAAIDVTGEAVRSPIGPQASVTATS
                     VTADSVSASFTVSAERTSGWGSEDVRWFFGDGKRFFDSSAALLFWGIGSSSVVFS"
BASE COUNT          505 a         1212 c         1059 g          508 t
ORIGIN      
        1 gacagggtcg tcggtcgcga agcgctcgag cgacgacgtc actcccgcac ctcgaaccag
       61 ggattgaggc acatagcccg gagccaagac gacgtcgagc tgccgccgga cctcgtccgc
      121 tgccgccgcg gccttcctcg caccgatctc ggccccgtcg tcgcctatga cgatcgccct
      181 ctctccttgg aggtcgaagg caaggctgtc cgtcgagtcg acgaggatca ggagcggttt
      241 ggcgaaggag tgagcgacgc ccagctcgta gtacacgttc gcgtttcgcc ccgtcagatc
      301 tgcaacgacg accttggcgt tgacgatcgc ctggatgatc tggacgctca ccgtgccggg
      361 ggtcggatct tcatcggcgc gggccaatcg cagaccccgc tcctcgagcg gtggcttcag
      421 aacgaagtcg cagagctcgt ccgttcgctc gcgggtctgt gaaccggggc tgccgatctg
      481 gcagatgacg aacgccgcgt caccattagc caacggcttt cccacttcgc gagaggccgc
      541 cgaaacctag aacgttgtcg tcacccacgt gcacgaattg tcacacgcgc cgcgcccggg
      601 ccacgagctc gcccccgcag ttcggacaca ccgacgccat ctctcgggcg caagcggaac
      661 agaacgtgca ctcgtagctg cagatgaacg cttcgtcgtt cggagccagc cccgtcccgc
      721 agcgttcgca cgagccccgc atctccagtg ccattacttc aagcgcgccc ccgcgggttg
      781 ggcagcggcg tcaggacctc ctccagcttc tcccgcaagt gctggtagtt cggcggcagc
      841 gagagcgact cgcccaggct ctcgagcggc tcgtccgccg tgaagcccgg ccccatcgtc
      901 gctatctcga acaggatccc cgacggctcc cggaagtaga tcgacttgaa gtagaaacgg
      961 tcgatcaccg gcgtgacctg ggcccccgca ccggcgaccc gctcgcgcca cgcgtcgtgc
     1021 tcgtctatcg gtgacgacca ggcgacgtgg tggacggtcc cggccccgtt cagtccgcgc
     1081 gcctcggggg ccgggtcgta gccgtagaac gagccccgct cctcgccgcg ggccgtccac
     1141 tggccagggt ccgactccgt gaagccgagg ccgtctcgca ggaactccgc gctctccgaa
     1201 gcatccgcgc tgtacgcccg cacgccgtcg aagccgagca gcgcgtgctc cgcggggacg
     1261 tccgggtggc tcgcccgcag cgggggctcc gacgagtccg tgacccgcag ctcgagcccc
     1321 aggccctcgg ggtcctcgaa cctcaggtag ccctcgcccc gctcgacctc gacgcccgcc
     1381 tcggcgaggc ggccggccca gaagtcgagc gactccggcg acgccacccg ccacacgatg
     1441 cggtggacca tcccggcccc cgcacggccg cggctcagcc cggggtactc gaagaacgtg
     1501 atgtcggagc cggggctgcc cttctcgtcg gcgtagaaca ggtggtagac ggtcgggtcg
     1561 tcctggttga cggtcttctt gaccatcctc agcccgagcg tgccggcgta gaactcgacg
     1621 ttcccggggg cgtccccggt gatcgccgtg atgtggtgga tcccctcgag tctcattcgc
     1681 ccgctcctcg atcggcgtcg taagtcgtct catccgggga acagggcccc ctcggaggct
     1741 attccgggcc gagaccggcc gcgcccccga gcaggtcagg cccctgcccc cgtctaaccc
     1801 tctctacgtg cgaaaggtcc tcgcagcggt gctcgtatgc ctggcgacgc tgggcggacc
     1861 cgccgcgcac gcggcccagc agctctcggc cgacgatccg ccgaccgccg agggcaactt
     1921 ctacgagccc gaccagcagt gcgacgacca gacgctgaag tcgaacggca agcccgtgat
     1981 caaggtcgcc ttctgcatct acttcttcgc gttcgacccc ctctgggagc tggacctcaa
     2041 caacgactac ggcgtcgcgt gggcgcaggc gacgttcgac gcccttcccg gctactgcac
     2101 gagcgagctc agcttctaca tcgagatccc cgagggcggg ggctaccaga tgcacgcgcg
     2161 ggtccccggc gagacgatca cgacccggtc gacgacgtcc acgctcgtcc acctcggggc
     2221 cacggcggcg gagaacgcga tccagcccgg aagcgtctcg caggcgctcg agctgctccc
     2281 cggcaagctg acgcccacgg ggaaggccgg cgagcctcgg gtcggggtgt cgtggaaggg
     2341 ccggaccggc gcaaagctcg cgttcgccac gggggccgag gtctcgtgga actggctctc
     2401 ggcgccgccg gagatccggc tcggggccga cacgatcaag ctgacgtcgg ggaagggctg
     2461 ttagttagag cgccccagac cggatttgaa ccggcgacct tctccttgac agggagatga
     2521 gcactcctag ctgctccact ggggcctgaa tttctgaggc taacaggtga tatccgctga
     2581 cacgacggtg acgcaccggt gccagaggac cgagggccct catatcgaac ccttcaagcc
     2641 atgggaccca cgatgcggca cctcagtgca ctcgccgcgg ccgtcgtcgt cgccgcgtcg
     2701 ctcagccctt ccgccgcggc agccccccgg acccgctcga tcgagctgac cgactgctcc
     2761 gagcacaccg cgttcttccc gatcccgcgg gcacagggcc gggactacgt gcccgccggc
     2821 ttcgaccccg ccgccgctcc ggccgacaac gacaacctca cgaacttcta cgtcacgtcg
     2881 tgggtgtgcg gcgacccgga gaacccggcc ctcgagctcg cattgaccca cctcgtcgtc
     2941 gacccacccc gggacgcttc ggcgagcaac cgctatctcg tcgacgtcgt cgccgagggc
     3001 cctgcggccg cgtcgctgcg gaaggtcacg tgcgtcccgg cggaggccgc ggcgatcgac
     3061 gtcaccggcg aggccgtgcg gtctcccatc ggcccccagg cgtcggtcac ggccacctcc
     3121 gtcaccgccg actcggtctc cgcgagcttc accgtgagcg ccgagcgcac cagcggctgg
     3181 ggctcggagg acgtccggtg gttcttcggc gacggcaagc gcttcttcga ctcgagcgcc
     3241 gcgctgctgt tctgggggat cggctcgtcg tcggtcgtct tctc
//
LOCUS       DASQDO010000494         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_114063, whole genome shotgun sequence.
ACCESSION   DASQDO010000494 DASQDO010000000
VERSION     DASQDO010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114063"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..874)
                     /locus_tag="VEV43_13605"
     CDS             complement(68..874)
                     /locus_tag="VEV43_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYP24600.1"
                     /translation="MPQQPSLKDLLSVAADAAYLAGRRTLAYFNAEVAVETKSDDTPV
                     TRADREAELVIRERVTRFFPDHAVLGEEHGETSGNPEYRWIIDPIDGTKSFIHGVPLY
                     GVLIGVEVRGRPSVGVIYAPGTDELVAAADGLGCTCNGRTSRVSRVERIEDATVLAGS
                     ITRALARSDAYEKLASRAKLNRGWGDAYGYLLVATGRAEVMLDPVLNPWDCAPLPPIL
                     REAGGRFTTWAGEETIWGPDGVATNGALNEHVLSVLRAETRRADANMKGD"
     gene            complement(945..>1822)
                     /locus_tag="VEV43_13610"
     CDS             complement(945..>1822)
                     /locus_tag="VEV43_13610"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYP24601.1"
                     /translation="KTAAFKGQEHPIEELTKDSFKGVQVALFSAGGSISKEFAPSAVK
                     AGAVVVDNTSYFRMKDGIPLVVPEVNGDQIAKHNGLIANPNCCTIIMVVPLWPLHKVN
                     RIKRVVVSTYQSVSGAGAWGLYELDAQMKAYATGQPIAKEKFPHQIVNNLFSHNTKVA
                     DNGYNEEENKMVAETRKIFADPKIQVSPTCIRVPIPRAHSESINVEFERPMTPDQVRA
                     VLAKAPGIRIVDDADKNHFPMPLEASGQDDVLVGRIRQDISRDDGRGIDLFVSGDQVR
                     KGAATNAVQIAERLV"
BASE COUNT          234 a          630 c          625 g          333 t
ORIGIN      
        1 gggaggaacg accggagcgg agccgcagaa tctccgcgcg aggcgccccc ccgagccccg
       61 ggaacgttca gtcgcccttc atgttcgcgt ccgcgcggcg ggtttccgcg cgaagcaccg
      121 acagcacgtg ttcgttcagc gccccgttgg tcgcgacgcc gtccgggccc cagatcgttt
      181 cctcgcccgc ccaggtggtg aaccgcccgc cggcctcgcg gaggatgggc ggtagcgggg
      241 cgcagtccca cgggttgagc accgggtcga gcatgacctc ggcccggccc gtggcgacga
      301 ggaggtagcc gtaggcgtcg ccccagccgc ggttgagctt cgcgcggctc gcgagcttct
      361 cgtaggcgtc cgaccgcgcg agcgcgcggg tgatgctgcc ggcgagcacc gtcgcgtcct
      421 cgatccgctc gacccgcgac acccggctcg tccgcccgtt gcacgtgcac cccagcccgt
      481 cggcggccgc cacgagctcg tccgtgcccg gcgcgtagat cacgccgacg ctcggccgcc
      541 cgcggacctc gacgccgatg aggacgccgt agagcggcac gccgtggatg aaggatttcg
      601 tgccgtcgat cgggtcgatg atccagcggt actcggggtt gccgctcgtc tccccgtgtt
      661 cctcgccgag caccgcgtgg tccgggaaga accgcgtgac ccgctcccgg atgacgagct
      721 cggcttcccg gtcggcccgg gtcaccggcg tgtcgtcgct cttggtctcg accgccacct
      781 cggcgttgaa gtaagcgagc gtgcgccgcc ccgcgagata agcggcgtcg gcggcgacgg
      841 agaggaggtc tttgagcgag ggttgctgcg gcaaaaacgc ggtcctttcc gtgggcttgg
      901 tttgtcgaga cgcgttgtcg gtccaggtgc gcggtccggg tttctcacac caaccgctcc
      961 gcgatctgca ccgcgttcgt cgcggcgccc ttgcgcacct ggtccccgct cacgaacagg
     1021 tcgatcccgc gcccgtcgtc acgactgatg tcctggcgga tccgcccgac gagcacgtcg
     1081 tcctgcccgc tcgcttcgag gggcatgggg aaatggttct tgtccgcgtc gtcgacgatg
     1141 cgaataccgg gcgccttggc cagcaccgcg cgcacctggt cgggcgtcat cgggcgctcg
     1201 aactcgacgt taatgctctc gctgtgggcc cgggggatcg ggacgcggat gcaggtcggg
     1261 gacacctgga tcttcgggtc ggcgaagatc ttccgcgtct cggcgaccat cttgttctct
     1321 tcctcgttgt agccgttgtc ggcgaccttc gtgttgtggc tgaagaggtt gttcacgatc
     1381 tggtgcggga acttctcctt ggcgatcggc tgcccggtcg cgtacgcctt catctgggcg
     1441 tcgagctcgt agagccccca cgcgccggcc ccgctcaccg actggtacgt gctgacgacg
     1501 acccgcttga tccggttcac cttgtgcagc ggccagagcg gcaccaccat gatgatggtg
     1561 cagcagttgg ggttggcgat caggccgttg tgtttggcga tctggtcgcc gttgacctcg
     1621 gggacgacga gggggatgcc gtccttcatc cggaagtagc tcgtgttgtc gacgacgacc
     1681 gcgccggcct tcacggcgct cggggcgaac tccttgctga tcgacccgcc ggccgagaac
     1741 agcgcgacct gcacgccctt gaagctgtcc ttggtcagct cctcgatcgg gtgctcctgc
     1801 cccttgaacg cggccgtctt cc
//
LOCUS       DASQDO010000495         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_603137, whole genome shotgun sequence.
ACCESSION   DASQDO010000495 DASQDO010000000
VERSION     DASQDO010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603137"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEV43_13615"
     CDS             <1..213
                     /locus_tag="VEV43_13615"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HYP24602.1"
                     /translation="KSSGLAVEDRIELWISGDAGVSDALRAHEGLIVREVLATSTSFE
                     APPEGAAVDEVALGDGSVRVGLRKSG"
     gene            complement(169..561)
                     /locus_tag="VEV43_13620"
     CDS             complement(169..561)
                     /locus_tag="VEV43_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP24603.1"
                     /translation="MRPVNVLIADDDAGIRSFLSVALVHEDGVGEVREATNGDEAVAM
                     CRDWQPDVVVLDYWMPVLDGGGAARQIRIDNPNVRIVAFSGVLQGTPDWADHHFLKGE
                     LPDLELVVRLGRDSLAGLAEPDADGAVA"
     gene            complement(671..844)
                     /locus_tag="VEV43_13625"
     CDS             complement(671..844)
                     /locus_tag="VEV43_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24604.1"
                     /translation="MTLLVGGAALLAVGAGVTLILGWSSDDAVLVWASLIASGLAALL
                     LVAAARSAPPDDD"
     gene            complement(873..1907)
                     /locus_tag="VEV43_13630"
     CDS             complement(873..1907)
                     /locus_tag="VEV43_13630"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HYP24605.1"
                     /translation="MDLTARDIHEKQFHDAWRGYNQEEVDDFLDRVAEVLDRVQRENA
                     TLQTRVNELDSAVAASRDTEEMLKKTLVGAQQAAEEAIATAKAKASDLITEAEQRARR
                     SIEEANERVQTAEAEARRKTLEAEREHERRRTELNANIERLQGLENELKNRIKGFLDQ
                     QAQALQGLEIVGPPPSILGPAAIKAAGETLAGETPGPGPAPATGAAPGGETPSTEGGP
                     SETERSTEGGTGPAAGETPAGTGGSGEGDATAATGGPETAAPVHGETRDEGAPERPGS
                     PEGGAPNGTPGPNASRSNGSGLVDDSTAGDAARVVTLPDAVSSGAGNVPAEAGHHRRG
                     MKGLFWRDEG"
     gene            complement(1957..2214)
                     /locus_tag="VEV43_13635"
     CDS             complement(1957..2214)
                     /locus_tag="VEV43_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HYP24606.1"
                     /translation="MEPIEILCIALQIYTLILFARIILSWVTMFWSPPPGLSPAIRLV
                     YDLTEPIMGFFRRYIPAVGGLDLSPIFIFIILNIVSRQIGC"
     gene            complement(2227..2718)
                     /locus_tag="VEV43_13640"
     CDS             complement(2227..2718)
                     /locus_tag="VEV43_13640"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HYP24607.1"
                     /translation="MAGVWRKTLIYLGLVEDDELDEYAYEEHEEEEPPRKQSRRAAAA
                     APVREPDEAFAPRRDAGVRPIQSQPQARFHLVNPTQFKADAQEVGDKFREGFSVLMNL
                     HAAEARERQRLIDFASGLAYGMGGGIQPAGENVILITPPGVQVSAEERQRFLEERGFF
                     KQA"
     gene            complement(2743..>3219)
                     /locus_tag="VEV43_13645"
     CDS             complement(2743..>3219)
                     /locus_tag="VEV43_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HYP24608.1"
                     /translation="DRVRWHFVGPLQSNKVRNVVGVAELVHSVDRYGLGEAIARRARS
                     RGIVQDVLVEVNTGGEATKHGVEPAGAVRLAEEVAALDGIAVRGLMTIPPRTDDPDGA
                     RPYFRDLVALRDLLAESVPGATELSMGMSADFEQAIEEGATIVRVGEAIFGPRHVP"
BASE COUNT          414 a         1143 c         1167 g          495 t
ORIGIN      
        1 aagtcgagcg ggctcgcggt cgaggaccgc atcgagctgt ggatctccgg cgacgccgga
       61 gtgtccgacg cgctgcgcgc gcacgaaggg ttgatcgtgc gcgaggtgct cgcgaccagc
      121 acctcgttcg aggccccgcc cgagggggcc gcggtcgacg aggtcgcgct aggcgacggc
      181 tccgtccgcg tcgggctccg caagtccggc tagcgagtcg cgtccgagcc gcacgacgag
      241 ctcgagatcc gggagctcgc ccttcaggaa gtggtggtcg gcccagtcgg gggtcccctg
      301 cagcacgccg gagaacgcga cgatccgcac gttcgggttg tcgatgcgga tctgccgggc
      361 ggcgccgccc ccgtcgagga ccggcatcca gtagtcgagc acgacgacgt ccggctgcca
      421 gtcccggcac atcgcgacgg cttcgtcccc gttcgtggcc tcgcggacct cgcccacgcc
      481 gtcctcgtgc accagcgcga cgctgaggaa gctgcggatc cccgcgtcgt cgtctgcgat
      541 cagtacgttc accggtctca cgtcgcgccg catccttttc gagccatgtc ctctggaacg
      601 cgagtgtgag ggagcgggcg cgcgagcgga ataagcaaat cacgcacgcg tgagccccgg
      661 cccacccgtc tcagtcgtcg tcgggagggg cggagcgggc cgccgccacg aggagaagcg
      721 cggcgagccc gctggcgatc agcgaggccc agacgagcac ggcgtcgtca gacgaccacc
      781 cgaggatcaa agtgacgccg gccccgacgg ccaggagggc cgcacccccg acgaggagag
      841 tcatcgcgta gctcggtggc cgcccgtacg ggctagccct cgtccctcca gaacaggccc
      901 ttcatcccgc ggcggtgatg ccccgcctcg gcggggacgt tcccggcgcc gctggatacc
      961 gcgtccggca gagtcacgac ccgcgcggcg tcgcccgccg tggagtcgtc gacgagcccg
     1021 gagccgttgg accgggacgc attcgggccg ggcgtgccgt tcggcgcacc gccctcgggc
     1081 gagcccggcc tttcgggagc gccctcgtcc cgggtctcgc cgtggacggg ggccgcggtc
     1141 tcgggccctc cggttgcggc cgtcgcgtcg ccctcgccgg agccacccgt cccggcgggg
     1201 gtctcgcccg cggccgggcc ggtcccacct tcggtcgagc gttccgtttc ggaggggccg
     1261 ccttcggtcg agggagtctc gccgccggga gctgccccgg tcgccggtgc cgggccgggg
     1321 ccgggcgtct cgcccgccag cgtctcgccg gcggccttga tcgccgccgg gcccaggatc
     1381 gacggcggcg ggcccacgat ctcgagcccc tgcagggcct gggcctgctg gtccaggaag
     1441 cccttgatcc ggttcttcag ctcgttctcg aggccctgga gccgctcgat gttcgcgttg
     1501 agctcggtcc ggcggcgctc gtgctcgcgc tcggcctcga gcgtcttgcg ccgggcctcg
     1561 gcctcggcgg tctggacgcg ctcgttggct tcctcgatcg accggcgggc gcgctgctcg
     1621 gcctcggtga tgaggtcgct ggccttcgcc ttcgcggtcg cgatcgcctc ctcggcggcc
     1681 tgttgcgcgc cgacgagggt cttcttcagc atttcctcgg tgtcgcggct ggcggcgacg
     1741 gccgagtcga gctcgttcac gcgggtctgg agcgtcgcgt tctcgcgctg gacgcgatcg
     1801 aggacctcgg cgacgcggtc gaggaagtcg tcgacctcct cctggttgta gccgcgccag
     1861 gcgtcgtgga actgcttctc gtggatgtcg cgtgccgtca gatccatacc cgggagtatc
     1921 gcatcgggtc gggccgaaaa cccggtttcg cgggggctaa cagccgatct ggcggctgac
     1981 gatgttcagg atgatgaaga tgaatatcgg cgagaggtcg aggcccccga ccgcggggat
     2041 gtagcggcgg aagaaaccca tgatcggctc ggtgaggtcg tacacgagcc ggatcgccgg
     2101 cgacagcccg ggcggcggcg accagaacat cgtcacccac gacaggatga tccgcgcgaa
     2161 caggatcagc gtgtagatct ggagcgcgat gcagaggatc tcgattggct ccacggccgc
     2221 ggtcccctaa gcctgcttga agaagccgcg ctcttcgagg aagcgctgtc tctcctccgc
     2281 cgagacctgg acccccggag gcgtgatcag gatcacgttc tcgcccgcgg gctggatgcc
     2341 gccgcccatg ccgtacgcga ggccgctggc gaagtcgatg agccgctgcc gctcgcgcgc
     2401 ctccgccgcg tggaggttca tcaggacgga gaagccctcc cggaacttgt cgccgacctc
     2461 ctgcgcgtcc gccttgaact gggtcgggtt gacgaggtgg aagcgggcct gcggctgtga
     2521 ctggatcggg cgcacgccgg cgtcgcggcg gggggcgaag gcctcgtcgg gctcgcgcac
     2581 gggggccgcg gcggcggcgc ggcgcgactg cttgcgcgga ggctcctcct cctcgtgctc
     2641 ctcgtacgcg tactcgtcga gctcgtcgtc ctcgaccaac ccgaggtaga tgagcgtctt
     2701 cctccacacg ccggccatgg ggggcaggat atcccggcgg ggttacggga cgtggcgggg
     2761 gccgaatatc gcctcgccga cccgcacgat cgtggccccc tcctcgatgg cctgctcgaa
     2821 gtccgcgctc atccccatcg agagctcggt cgcgccgggg accgactccg ccaggaggtc
     2881 acgcaacgcc acgaggtccc ggaagtaggg ccgcgcgccg tcggggtcgt ccgtgcgggg
     2941 cgggatcgtc atgagtcccc tgaccgctat gccgtcgagc gcggcgacct cctcggcgag
     3001 ccggacggcg ccggcgggct cgacgccgtg cttggtcgcc tctcccccgg tgttcacctc
     3061 gacgagcacg tcctgcacga tcccccggga tcgtgcgcgc cgggcgatcg cctcgccgag
     3121 cccgtagcgg tcgacggagt gcacgagctc ggccacgccg accacgttac ggaccttgtt
     3181 cgactggagg ggcccgacga agtgccagcg gaccctgtc
//
LOCUS       DASQDO010000496         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1153779, whole genome shotgun sequence.
ACCESSION   DASQDO010000496 DASQDO010000000
VERSION     DASQDO010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153779"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VEV43_13650"
     CDS             <1..635
                     /locus_tag="VEV43_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP24609.1"
                     /translation="AEAVAGELGLALYVIDLSTVVDKYIGETEKNLERIFAEAAHVNG
                     VLLFDEADALFGKRSGVEDARDRYANVEVSYLLQRIESFDGVALLTSNLRGNVDEAFA
                     RRLDAVVEMPLPDEAARLRLWHVNLPQTLPRDDGVDLTFLARFELSGGNVRNASVSAA
                     YRAAAADRAVTMHDLVRGVAQEYTKLGRLCTEGEFGDYHALVTGASAIAR"
     gene            732..>2231
                     /locus_tag="VEV43_13655"
     CDS             732..>2231
                     /locus_tag="VEV43_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HYP24610.1"
                     /translation="MPTYLSPGVYVEEVAAGSRPIEGVGTAVAAFVGLAQSGPFNTPT
                     LITNWTQFTGIFGDFVEGSYLAHAVYGYLLNGGGACYAVRIGEDLAPGPAARAELPSA
                     ADASQPALRVAAAGPVDDGDVEVEVRPPAPGAEDESAFDVVVKKAGTVVETFENLTAG
                     EGDRNVVTVVNAQSEVITLEQTAAGDTVAAPAPVTVTLAPAAPAPAALAPDDYVGNAA
                     DRTGFAGLEAIDGVTMLCVPDLMAAYQRGAIDLEGMKAVQLAMIAHCELMGDRMAVLD
                     PPPGLNAQQIMDWRTQYAGYDSKYATLYWPWIKVFDPATGTNVFAPPSGYVAGVWGRN
                     DDTRGVHKAPANETIRGAIDLEINITKSEHDQLNPSGINCLRSFPGRGIRIWGARTLS
                     SDAAWRYVNVRRLFNYLEESILEGTQWAVFEPNDHALWAKIRRTIAAFLTNEWRKGAL
                     FGQTPDQAFFVKCDEETNPPEIVDSGQVVCQVGVAPVKPAEFVVFQLSQF"
BASE COUNT          343 a          813 c          762 g          313 t
ORIGIN      
        1 cggccgaggc cgtcgccggg gagctgggcc tggcgctgta cgtgatcgac ctgtccacgg
       61 tcgtcgacaa gtacatcggt gagaccgaga agaacctcga gcggatcttc gccgaggccg
      121 cgcacgtgaa cggcgtcctg ctgttcgacg aggccgacgc gctgttcggc aagcgctcgg
      181 gcgtggagga cgcgcgcgac cgctacgcga acgtcgaggt gtcgtacctg ctgcagcgca
      241 tcgagtcgtt cgacggcgtc gctctcctga cgtcgaacct gcgcgggaac gtcgacgagg
      301 ccttcgctcg acgcctcgac gcggtcgtcg agatgcctct tcccgacgag gccgcgcgcc
      361 tgcgcctgtg gcacgtgaac ctgccgcaga cgctcccgcg cgacgacggc gtcgacctca
      421 cgttcctggc gcggttcgag ctgtccggcg gcaacgtccg gaacgcgagc gtctccgccg
      481 cataccgggc cgcggcggcg gaccgggccg tgacgatgca cgacctcgtc cgcggagtgg
      541 cgcaggagta cacgaagctg ggccgcctct gcaccgaagg cgagttcggc gactaccacg
      601 cgctcgtcac cggcgcctcc gcgattgccc gttagggcag ccgccgcttc cccctcgtgt
      661 gcccggctgc acctagccgg gccggccccc gtggccgagc ctgaacccct cagtccgacg
      721 aaggaggcga catgcccact tacctgtcac ccggtgtcta cgtcgaggag gtcgcggcgg
      781 ggtcgcggcc gatcgagggt gtcggcaccg ccgtcgccgc gttcgtcggc ctcgcccaga
      841 gcggcccctt caacaccccc acgctgatca cgaactggac ccagttcacc ggcatcttcg
      901 gcgacttcgt cgagggctcg tacctcgccc acgccgtgta cggctacctc ctcaacggcg
      961 gcggcgcgtg ctacgcggtc cgcatcggcg aggacctcgc gccgggcccg gcggcgcgag
     1021 cggagctccc gagcgcggcc gacgcgtcgc aacccgcgct ccgcgtcgcg gccgccggcc
     1081 cggtggacga cggcgacgtc gaggtcgagg tgaggccgcc cgcccccggt gccgaggacg
     1141 agtccgcgtt cgacgtcgtc gtgaagaagg ccggaaccgt cgtcgagacg ttcgagaacc
     1201 tcaccgcggg cgagggagat cgcaacgtcg tcacggtggt caacgctcag tccgaggtga
     1261 tcacgctcga gcagacggcg gcgggcgaca cggtagcggc ccccgctccg gtgacggtga
     1321 ccctcgcgcc tgcggccccc gcgcccgccg cgctcgcgcc cgacgactac gtcggcaacg
     1381 ccgcggaccg caccgggttc gccggcctcg aggcgatcga cggcgtgacg atgctgtgcg
     1441 tccccgacct gatggccgcg taccagcgcg gcgcgatcga cctcgagggg atgaaggccg
     1501 tgcagctcgc gatgatcgcc cactgcgagc tcatgggcga tcgcatggcg gtcctcgacc
     1561 cgccgccggg gctgaacgcg cagcagatca tggactggcg gacccagtac gccggctacg
     1621 actcgaagta cgccacgctc tactggccct ggatcaaggt cttcgacccc gcgaccggca
     1681 cgaacgtgtt cgctccgccg agcggctacg tcgcaggcgt gtggggacgc aacgacgaca
     1741 cccgcggcgt ccacaaggct cccgcgaacg agaccatccg cggcgcgatc gacctcgaga
     1801 tcaacatcac gaagagcgag cacgaccagc tgaacccttc ggggatcaac tgcctgcgca
     1861 gcttcccggg acgcggcatc cgcatctggg gcgcacgcac gctctccagc gacgcggcgt
     1921 ggaggtacgt caacgtccgg cgtctcttca actacctcga ggagtcgatc ctcgaaggga
     1981 cgcagtgggc cgtgttcgag cccaacgacc acgcgctgtg ggcgaagatc cgccgcacga
     2041 tcgccgcgtt cctcacgaac gagtggcgca agggggcgct gttcgggcag accccggacc
     2101 aggcgttctt cgtcaagtgt gacgaggaga cgaacccgcc cgagatcgtc gacagcggcc
     2161 aggtcgtctg ccaggtcggc gtcgcgcccg tcaagcccgc cgagttcgtc gtcttccagc
     2221 tctcccagtt c
//
LOCUS       DASQDO010000497         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_236583, whole genome shotgun sequence.
ACCESSION   DASQDO010000497 DASQDO010000000
VERSION     DASQDO010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236583"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VEV43_13660"
     CDS             complement(<1..958)
                     /locus_tag="VEV43_13660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYP24611.1"
                     /translation="MATSSVAAVRPRLVTPVFVLITLSTFAYFVSVGALIPTLPLYVE
                     GPLGGGDVAVGLGIGAFSLSAVLLRPFVGRLSDLRGRRLLLVAGAGLVTISVAGYVVV
                     SSLIPLLLLRLLTGAGEAFFYVGAASAINDIAPDERRGEALSFFSLALYAGMAIGPIL
                     GEAALGVDRFDAAWLLAAGAAGVALLLGWKVPDTRPPDVVPGASPLIHRAALVPGTVL
                     AASVWGLAGFSTFVPLYARELGLSGSSYVFATYSGLVLALRLFGAQIPDRLGTRLTSR
                     LALSASALGMAVMGMVESPVGLFAGGAIFGVGQALAFPALMSI"
     gene            1023..1421
                     /locus_tag="VEV43_13665"
     CDS             1023..1421
                     /locus_tag="VEV43_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HYP24612.1"
                     /translation="MILLGGGLIGILLLALWAYCIFDVISTDEALMRNMPKVMWLIVV
                     ILIPTVGAVAWLLLGRPIGAGLRPGDTTPHRPMPRPSRPAPRPLAPDDDPTFLARLDD
                     ETRRLRAWEDDLKRREDELRGKDDGDTANE"
     gene            1418..>1780
                     /locus_tag="VEV43_13670"
     CDS             1418..>1780
                     /locus_tag="VEV43_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HYP24613.1"
                     /translation="MTTFGEAYSATRRSVIELVSGLSDEDAGERVPACPDWSVRDLVA
                     HLTGIAVDATKGELEGVGSPEWTQRQVDERKSMPLDDLLAQWEEAGSQIDGALEYFPK
                     AAASLFVGDTVTHEHDIRL"
BASE COUNT          328 a          650 c          601 g          201 t
ORIGIN      
        1 ctatcgacat cagcgccggg aacgcgaggg cctggcccac cccgaagatc gccccgcccg
       61 cgaacaggcc taccggcgac tcgaccatgc ccatcaccgc catgccgagg gccgaggcgg
      121 acagcgcgag acgagaagta aggcgcgtcc ccaatcggtc cgggatctgc gcaccgaaca
      181 gccgcagcgc tagcaccaga ccgctgtacg tcgcgaagac gtagctcgac ccggagagac
      241 ccagctcgcg cgcgtacaaa ggaacgaacg tcgagaagcc ggcgaggccc cacacgctcg
      301 ccgccagcac cgtccccggc accagcgcgg cccggtggat caacggcgac gctccgggca
      361 cgacgtccgg gggccgcgtg tcgggcacct tccaacccag cagcagcgcg acaccggcgg
      421 ctcccgcagc cagcagccac gccgcgtcga aacggtcgac gccgagggcg gcctcaccga
      481 ggatcggacc gatcgccatc ccggcgtaca gcgcgagcga gaagaagctc agcgcctcgc
      541 cgcgcctctc gtcgggcgcg atgtcgttga tcgccgacgc ggcccccacg tagaagaagg
      601 cctcgccggc tccggtcaga aggcgcagca acaagagcgg gatcaaagac gacacgacga
      661 cgtacccggc caccgagatc gtcacgagcc cggcccccgc gaccagcagc agacgccgtc
      721 cccgcaggtc gctcaagcgc ccgacgaacg gccgcagcag aacggccgac aagctgaacg
      781 ccccgatccc gagcccgacc gcgacgtcac cacctccgag cgggccctcg acgtacagcg
      841 gcaacgtcgg gatcagcgcg cccaccgaga cgaagtaggc gaacgtcgac agcgtgatga
      901 gcacgaagac cggcgtaacg agccgcgggc ggaccgcggc gaccgaggag gttgccatac
      961 gtgcccccaa cgcagggcgc tcgcacacga ttcctccggg ggccgtggac taagctcggc
     1021 ccgtgatcct tctcgggggc gggctgatcg gaatcctcct gctcgcgttg tgggcctact
     1081 gcatcttcga cgtgatctcg accgacgagg ccctcatgcg caacatgccg aaggtcatgt
     1141 ggctgatcgt ggtgatcctg atccccacgg tcggcgcggt cgcgtggctg ctcctcggcc
     1201 gtcccatcgg tgcgggtctg cgtccgggtg acacgacccc gcaccggccc atgccgcgcc
     1261 cgtcccgccc ggccccccgg ccccttgctc cggacgacga cccgacgttc cttgcgcggc
     1321 tcgacgacga gacgaggcgt ctgcgtgcgt gggaggacga cctcaagcgg cgcgaggacg
     1381 agctccgcgg caaggacgac ggcgacaccg cgaacgaatg acgaccttcg gcgaggctta
     1441 ctccgcgacg agacggagcg tgatcgagct cgtctccggg ctctccgacg aggacgcggg
     1501 cgagcgcgtc ccggcgtgcc ccgactggag cgttcgggac ctggtcgcgc acctcaccgg
     1561 catcgcggtc gacgcgacca agggtgagct ggaaggcgtc ggctcaccgg agtggacgca
     1621 acgtcaggtc gacgagcgca agtcgatgcc gctggacgat ctgctcgcgc agtgggagga
     1681 agcggggagc cagatagacg gggcgctcga gtacttcccg aaggccgcgg cgagcctctt
     1741 cgtgggcgac accgtgacgc acgagcacga catccggctc
//
LOCUS       DASQDO010000498         7390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_970978, whole genome shotgun sequence.
ACCESSION   DASQDO010000498 DASQDO010000000
VERSION     DASQDO010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970978"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..872)
                     /locus_tag="VEV43_13675"
     CDS             complement(81..872)
                     /locus_tag="VEV43_13675"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYP24614.1"
                     /translation="MDPQKLGSSGQKFRPRRSRSGRAGHVRGGTPIDSTYSKSDRETL
                     KAIYRLTRGGADAHTGALAEALGVSPGTATAAVKRLAERELLDHKPYKGVELTPTGRR
                     AAVASIRRHRIVERFLSDMLGYAWNEADRLAGDFEHELPQEVEDRLFVALDRPATCPH
                     GFPIPEPDVTEIPDMPPLYALEPGDVAVVAVPGSTDPDVVRFLETLGLRPGVKVTVRE
                     KHPFDGPIVLRVEGQDRTISERVAEQIYVRKEERKTTVRRKEKSA"
     gene            911..3358
                     /locus_tag="VEV43_13680"
     CDS             911..3358
                     /locus_tag="VEV43_13680"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HYP24615.1"
                     /translation="MLAAAREVLVTRPRLWVAAGLCALLAALPGAAAATFGAAGGVAF
                     LLTGDAGLALRIWEHGPGLSPAILGAAGICFVAGFVVWARLYAIAVYLSRPGEAPGWD
                     DARGATRRSWWTVLWMHVQTFAVLVVVAVAVSLLLALAGPAAFGTLILLGAVAFAIFR
                     TIVRILLSVALRAVVFDGVPALLAWRSGARFVKARRHDVAVTWVGLVAIGVSLWIGGR
                     LITPVLQETAFDYDATSGYELARQVVQLLVALPLETALLGLGVAAWTALYDGATIEPR
                     GRARAERRAGGEAEPWVRKALAAGVVLALVGNGGPTLIADAHDRALDEREDDVARADV
                     KPEDVADASPAGPPPGRGSGTVYVVEAVLEDDELEWTTTIDYVNRTGDTLDDVGVNVY
                     TAAYARPLRDIPFARDLVASDFNGEFQARARSGEISRFEVTAQGRETDADLDGTSVVV
                     DLPRPLEPLARTRLEIAMTMELPRFPERFGRWRDLTLLGNWIPHVAVRRDGEWLLPRF
                     GSVGDPFVSETADYQVRLTVDENEGVVGSGSLLSVEDAGGGARQWRFAAPDLRDAAFV
                     VGPFLRGLEADAGGTVVRSWYPAGRGLEGAANLEAAVSAVEYYTDTYGRLPWPEVEVV
                     ETEGRLGGMEYPGVVFVSSASEPFSGLPLLPDLVSYAGFEEARSRYVVAHELAHQWWY
                     ASVGSDQVEEPWLDEALAEASTRMWLEGEDGGERTWLMTNLSGAAEPSREAVRAGIGD
                     FASNEDYVDAIYHSGAEVLVELRRAVGQDTYDEIMRAWHLRNGLGTGTIDELARVVVD
                     VAGDGGRAFVERWLAPQ"
     gene            complement(3344..3766)
                     /locus_tag="VEV43_13685"
     CDS             complement(3344..3766)
                     /locus_tag="VEV43_13685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP24616.1"
                     /translation="MPSALEQTAPSVRLEIPPQSAYVAIARLAITSLGRAAGLDEERL
                     DDLKIAISESCANAVLSVQQAGVDAPIEISFNAGGDALEVVVGHDGPPPPEAADADSQ
                     GFSTQAVMSMALLRSLVDELDLSPRPGGGMQARLLLRR"
     gene            complement(3768..4118)
                     /locus_tag="VEV43_13690"
     CDS             complement(3768..4118)
                     /locus_tag="VEV43_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HYP24617.1"
                     /translation="MELGLDVRKMGTHAVVDVKGEIDVYTAPKLREKLIELVSEGSYD
                     VVVNLEGVDFLDSTGLGVLVGALKRVKAHDGSLSLVCTQDKILKIFKITGLTKVFPIH
                     SSVDEAAGASTQEA"
     gene            4264..4854
                     /locus_tag="VEV43_13695"
     CDS             4264..4854
                     /locus_tag="VEV43_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24618.1"
                     /translation="MSVVLPAMKKTIWPLLVAFSVAALSALWLLAADRVSVGAGRGVQ
                     GDAPIEQAAWQVKAYPAPGAAGITKDDRAVFTRERDEVVGAIQNLYDGLFLRRDAVNE
                     TVRATMTPAASKVLADSRIGLHDRVEDVKTTVRFVKVGIQVDDGLRAAAVVRVEAEAL
                     RKSSPIEVWHRAKLWLEKEDGDWKVVAFDAEQGRLR"
     gene            4851..5729
                     /locus_tag="VEV43_13700"
     CDS             4851..5729
                     /locus_tag="VEV43_13700"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYP24619.1"
                     /translation="MRRRVLILLVLVGVVAATAPMASSPVASSPLLVGRVHTRYQPTD
                     GKVFVAIIGSDARAGNPDNVNADAIHIAGINTKTMKGGVLNFPRDSYVSIPGYGSGRI
                     NEALHAGGPELVAKTLESITGITIDYWVMVGFEDFRNIISALDGVKMDIPQDVYDPGG
                     SGANLKAGVQHLQAEEALSYVRARKPFADGDIARTTHQADFLIAMLRKLRRQVEYSPT
                     TLFRWTATARRYARLDLSASEMFRLGVLASQVEPEDVGNVTVPVSYGFVGAASVVFIQ
                     PEAKEIYERFAKDGSL"
     gene            complement(5737..6840)
                     /locus_tag="VEV43_13705"
     CDS             complement(5737..6840)
                     /locus_tag="VEV43_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HYP24620.1"
                     /translation="MADTPPVAQRNLVVCLDGTRNEPENGCTNVVRIFDMAVKDESQV
                     VYYDPGVGTMGARSATTRAGKFLTQSFGSLLGHGIKDNIEEAYKFLMQSYRANDRIFV
                     FGFSRGAYTALALAGMLRTVGLLRPHLDNLIPYAIDMYAQAGGYERNPDGSMTPEERK
                     FWDERKEFNETFGNPDFPNRFDKKTRLVHFLGIWDTVKTVGWLNLPIRLQEAQWPFTR
                     NVSGVANARQALAIDEDRRPYGDYALNLDPEDKPERARQMWFAGVHSDVGGFYEDDHK
                     LSDIALQWIAEEAIEKELRVDEARFEFHVGVPRGGQLPDEPLGEIHYHPWYWYVPGRR
                     RRAIPPGHEVHPSVLKRVELTAGTPDAYEVDIP"
     gene            6957..>7390
                     /locus_tag="VEV43_13710"
     CDS             6957..>7390
                     /locus_tag="VEV43_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HYP24621.1"
                     /translation="MPPTHALNVDLAKIYTRDGDRFRYLKTIAWGAELFVREIAGDHV
                     RIGVTVRNESGQWRRVDGFIRVPRGSDLEAADLVIERENSRLLTVEFVDVQQGDAAIV
                     ESPSGKVVLIDGGESQLFARYLATRFRDTEPTKPKEIDEIVVT"
BASE COUNT         1052 a         2502 c         2629 g         1207 t
ORIGIN      
        1 cgcgaagaac agccacatca tctcggtgcc gccgagatcg aactcctggt accccccctc
       61 ggctgctagc aggagcggtt tcatgcgctc ttctcctttc ttcttacggt cgtctttctc
      121 tcttccttgc ggacgtagat ctgctctgcg acgcgctccg agatggtccg gtcctgccct
      181 tccacgcgca gcacgatcgg accgtcgaac gggtgcttct cacggaccgt gaccttcacg
      241 ccgggccgca ggccgagcgt ctcgaggaac cgcacgacgt cggggtcggt cgagcccggc
      301 accgcgacga ccgccacgtc ccccggctcg agcgcgtaga gcggcggcat gtcggggatc
      361 tcggtgacgt cgggctcggg gatcgggaac ccgtgcgggc acgtcgccgg acggtcgagg
      421 gcgacgaaca gacgatcctc gacctcctgc gggagctcgt gctcgaagtc gccggcgagg
      481 cggtctgcct cgttccacgc gtagccgagc atgtcggaga ggaagcgttc gacgatgcgg
      541 tggcgcctga tcgacgcgac cgcggcgcgc cgcccggtgg gcgtgagctc gacccccttg
      601 tacggcttgt ggtcgaggag ctcccgctcg gcgagccgct tcacggccgc cgtggccgtg
      661 cccggagaga cccccagggc ctcggcgagc gcgccggtgt gcgcgtcggc accgccgcgc
      721 gtgagccggt agatggcctt cagcgtctca cgatcggact tcgaatacgt cgagtcgatt
      781 ggtgttcccc cgcgaacgtg gccggcgcgc ccgcttctgg agcgtcgcgg cctgaatttt
      841 tgcccggagg aacctagctt ttgggggtcc aaaatgtcaa acggtgacga gcgtgcgtta
      901 gaaagcccgg atgctcgctg cggcccgaga agtcctggtc acgcggccgc ggctgtgggt
      961 ggccgccggt ctctgtgcgc tcctcgccgc cctccccggt gcggcggccg cgactttcgg
     1021 agccgcaggc ggcgtcgcct tcctcctgac gggggacgcc ggcctggcgc tacggatctg
     1081 ggagcacggc ccggggctat caccggcgat cctgggcgcc gctgggatct gcttcgtcgc
     1141 cggcttcgtc gtctgggccc gcctgtacgc catcgcggtc tacctgtccc ggcccggcga
     1201 ggcacccggc tgggacgacg cacggggcgc cacgcgacgg tcgtggtgga cggtcctgtg
     1261 gatgcacgtg cagacgttcg cagttctcgt cgtggtcgcg gtcgccgtct ccttgctgct
     1321 ggcgctcgcc gggccggcgg cgttcgggac gctgatcctg ctgggagcgg tggcgttcgc
     1381 gatctttcgg acgatcgtcc ggatcctgct gagcgtcgcg ctgcgcgcgg tggtgttcga
     1441 cggcgtgccg gcgttgctcg cgtggcgcag cggcgcgcgc ttcgtaaagg cgcgccggca
     1501 cgacgtcgcg gtcacgtggg tggggctcgt cgcgatcggg gtgtcgttgt ggatcggggg
     1561 gcggctgata acgccggttc tgcaagagac cgccttcgat tacgacgcga ccagcgggta
     1621 cgagctcgcc cgccaggttg tgcagctgct cgtcgcgctc ccgctggaga cggcgctgct
     1681 cgggctcggg gtcgctgcgt ggaccgccct gtacgacggc gccaccatcg agccgcgcgg
     1741 acgggcacgt gccgagcgac gcgcgggggg cgaggccgaa ccgtgggtgc gcaaggcgct
     1801 cgccgcgggc gtggtcctcg cgctcgtggg caacggcggg ccgacgctga tcgccgacgc
     1861 gcacgaccgg gcgctcgacg agcgtgaaga cgacgtcgcg cgagccgacg tcaagccgga
     1921 ggacgtcgca gacgcgtcgc ccgccggccc cccacccggg cggggctccg ggaccgtgta
     1981 tgtggtcgag gccgtcctcg aggacgacga gctcgagtgg acgacgacga tcgactacgt
     2041 caaccgaacc ggcgacaccc tcgacgacgt cggggtcaac gtctacacgg cggcgtacgc
     2101 gcggccgctg cgcgacatcc ccttcgcgcg cgacctcgtc gcgtccgact tcaacggcga
     2161 gttccaagcc cgcgcgcgca gcggcgagat ctctcgcttc gaggtgaccg cgcaggggcg
     2221 ggagacggac gctgacctcg acggcacgtc ggtcgtcgtc gacctgccgc gcccgctcga
     2281 gccgctcgct cggacgcgtc tcgagatcgc catgacgatg gagctgccgc gctttcccga
     2341 gaggttcgga cggtggcgcg acctgacgct cctcggcaac tggatcccgc acgtcgcggt
     2401 ccggcgcgac ggcgaatggc tcctcccgcg cttcggctcg gtcggcgacc cgttcgtgtc
     2461 cgagaccgcg gactaccagg tgcggctcac cgtcgacgag aacgagggcg tcgtggggtc
     2521 gggctcgttg ctctcggtcg aagacgccgg cgggggcgcc cggcagtggc ggttcgcggc
     2581 ccccgacctg cgcgacgcgg ccttcgtcgt cgggcccttc ctgcgcgggc tcgaggccga
     2641 cgccggcggg accgtcgtgc gctcctggta tcccgcgggg cgaggcctgg aaggggccgc
     2701 gaacctcgag gctgcggtgt cggccgtcga gtactacacg gacacgtacg ggcgcctccc
     2761 gtggccggag gtcgaggtcg tcgagaccga gggccggctc ggcgggatgg agtatcccgg
     2821 cgtcgtcttc gtgtcgagcg cgtcggaacc gttctccggg ctcccgctgc tgcccgacct
     2881 cgtctcgtac gcgggcttcg aggaggccag gtcccgctac gtcgtcgcgc acgagctcgc
     2941 gcatcagtgg tggtacgcgt cggtcggaag cgaccaggtc gaggagccgt ggctcgacga
     3001 ggccctggcc gaggcgtcga cgcggatgtg gctcgaaggc gaagacggcg gcgaacggac
     3061 gtggctcatg acgaacctgt cgggcgcggc cgagccgtcg cgggaagccg tgcgcgccgg
     3121 catcggcgac ttcgcgtcca acgaggacta cgtcgacgcg atctaccact cgggtgcgga
     3181 ggtgctcgtc gagctgcggc gcgcggtcgg tcaggacacg tacgacgaga tcatgagggc
     3241 gtggcatctc cgcaacgggt tgggcaccgg cacgatcgac gagctggcgc gcgtggttgt
     3301 ggacgtcgcg ggtgacgggg gccgggcgtt cgtcgagagg tggctagcgc cgcagtagga
     3361 ggcgcgcttg catcccgccg ccggggcggg gcgacaggtc gagctcgtcg acgaggctgc
     3421 gaagcaacgc catcgacatc acggcctgcg tcgagaaccc ttgcgagtcg gcatccgcgg
     3481 cctccggcgg cggagggccg tcgtgcccca cgacgacctc gagcgcgtcg ccgccggcgt
     3541 tgaacgagat ctcgatcggc gcgtcgacgc cggcttgctg caccgacagc accgcgttcg
     3601 cgcacgactc ggagatcgcg atcttcaagt cgtcgagcct ctcctcgtcg aggcccgcgg
     3661 cgcgtcccaa cgacgtgatc gccaggcggg ctatcgcgac gtaagcggac tgcgggggga
     3721 tctcgagccg gacggacggt gccgtctgct cgagcgcaga gggcaagcta cgcctcttgc
     3781 gtagaggctc cggccgcttc gtcgaccgag gagtggatcg ggaacacctt cgtgagcccg
     3841 gtgatcttga agatcttgag gatcttgtcc tgcgtgcaca cgagcgagag gctgccgtcg
     3901 tgcgccttga cgcgcttgag cgcgcccacg aggacgccga ggccggtcga gtcgaggaag
     3961 tcgacgcctt cgaggttcac gacgacgtcg taggagccct cggagacgag ctcgatcagc
     4021 ttctcgcgga gcttcggggc ggtgtagacg tcgatctcgc ccttgacgtc gacgaccgcg
     4081 tgcgtcccca tcttccttac gtcgagcccg agctccatcc ggtcctccag ttctggccgt
     4141 ttgacatatg tgatttgagg gccgcatcct acccgccgac tcctgcgtcg acggaattcc
     4201 gccgatacgc ggggtcgtct cggtaccctc accaagccga ccgtcgggaa cgaggaaccc
     4261 cccttgagcg tcgttctccc tgccatgaag aagaccatct ggccactgct cgtcgccttc
     4321 tcggtggcgg cgctgtccgc gctgtggctg ctcgcggccg accgcgtctc ggtcggcgcg
     4381 ggacggggcg tccaggggga tgccccgatc gaacaggccg cctggcaggt caaggcgtac
     4441 ccggctcccg gcgcggcagg catcacgaag gacgaccgcg cggtgttcac ccgcgagcgg
     4501 gacgaggtcg tcggcgccat ccagaacctc tacgacgggt tgttcctgag gcgggacgcc
     4561 gtcaacgaga cggttcgggc gacgatgacg cccgcggcgt cgaaggtcct cgccgactcc
     4621 cgcatcggcc tccacgaccg ggtcgaggac gtcaagacca cggtgcggtt cgtcaaggtc
     4681 gggatccagg tcgacgatgg cttgcgcgcc gcggcggtcg tgcgcgtcga ggcggaggcg
     4741 ctgcgcaagt ccagcccgat cgaggtgtgg caccgcgcga agctctggct cgaaaaggag
     4801 gacggcgact ggaaggtcgt cgcgttcgac gcggagcagg ggcggctgcg gtgaggcggc
     4861 gggtgttgat cctgctcgtg ctcgtcggcg tcgtcgccgc gaccgcgccc atggcttcgt
     4921 cgccggtcgc gtcctcgccg ttgctcgtcg gtcgcgtgca cacgcgctac cagcccaccg
     4981 acggcaaggt cttcgtcgcg atcatcggga gcgacgcgcg cgcgggcaac cccgacaacg
     5041 tcaacgccga cgcgatacac atcgcgggca tcaacacgaa gacgatgaag ggcggcgtgc
     5101 tcaacttccc gcgcgactcg tacgtgtcga tccccggcta cggctccggg cgcatcaacg
     5161 aggcgctgca tgcagggggg ccggagctcg tcgcgaagac gctcgagagc atcaccggca
     5221 tcacgatcga ttactgggtg atggtcggct tcgaagactt ccgcaacatc atctcggccc
     5281 tcgacggcgt gaagatggac atccctcagg acgtctacga ccccggcgga tcgggcgcga
     5341 acctgaaggc cggcgtgcag cacctgcagg ccgaggaggc gctctcgtac gtccgcgcgc
     5401 gcaagccgtt cgccgacggc gacatcgcgc ggacgacgca ccaggccgac ttcttgatcg
     5461 cgatgctgcg caagctccgg cgtcaggtcg agtacagccc gacgacgctg ttccgctgga
     5521 ccgcgacggc gcggcgctac gcgcgcctcg acctctcggc gtctgagatg ttccgcctcg
     5581 gcgtgctcgc gtcgcaggtc gagcccgagg acgtcggcaa cgtgaccgtc cccgtgagct
     5641 acggcttcgt gggggccgcg agcgtggtgt tcatccagcc ggaggcgaag gagatctacg
     5701 agcgcttcgc caaggacggc tcgctctaag ccgtcgctac gggatgtcga cctcgtaggc
     5761 gtccggggtc ccggccgtca gctcgaccct cttgaggacg ctcgggtgca cttcgtgccc
     5821 gggtgggatg gcgcgacggc gacgcccggg gacgtaccag taccaggggt ggtaatggat
     5881 ctccccgagc ggttcgtcgg gaagctgccc gccacgcggg accccgacgt ggaactcgaa
     5941 ccgcgcctcg tcgacccgga gctccttctc gatcgcctcc tcggcgatcc actggagggc
     6001 gatgtcggag agcttgtggt cgtcttcgta gaagcctccg acgtcgctgt gcacgcctgc
     6061 gaaccacatc tgccgcgccc gctccggttt gtcttcggga tcgaggttca gcgcgtagtc
     6121 cccgtacggc cggcgatcct cgtcgatcgc cagcgcttgc cgcgcgttgg cgacgcccga
     6181 cacgttgcgc gtgaacggcc actgcgcctc ctgcaaccgg atcggcaggt tcaaccaccc
     6241 cacggtcttg accgtgtccc agatgccgag gaagtggacg agccgtgtct tcttgtcgaa
     6301 tctgttcggg aagtcggggt tgccgaacgt ctcgttgaat tccttccgct cgtcccagaa
     6361 cttgcgttcc tccggcgtca tcgagccgtc gggattccgt tcgtagccgc cggcctgcgc
     6421 gtacatgtcg atcgcgtacg ggatgaggtt gtcgaggtgc gggcggagaa gtcccacggt
     6481 tcgcagcatc ccggccagcg ccagcgcggt gtacgcgccg cggctgaacc cgaagacgaa
     6541 gatgcggtcg ttcgcccggt agctctgcat caggaacttg taggcctctt cgatgttgtc
     6601 cttgatcccg tggccgagaa gcgagccgaa cgactgcgtg aggaactttc cggcgcgggt
     6661 cgtcgcgctc cgagccccca tcgtcccgac gcccgggtcg tagtagacga cctggctctc
     6721 gtccttgacc gccatgtcga agatgcgcac gacgttcgtg cagccgttct cgggctcgtt
     6781 cctggtgccg tcgagacaga cgacgaggtt cctctgcgca accggtggtg tgtccgccat
     6841 ccctccccct ctcgctcctc cggcgactgt cgcacgccgg ttcgagcgcg tacaaccggg
     6901 ttcgtttccc cggtctccct tgacgcccag aacacgcagg ggaaaagtag agggccatgc
     6961 ctcccaccca cgcgctgaac gtcgacctcg cgaagatcta cacccgcgac ggcgaccgct
     7021 tccggtacct caagacgatc gcctgggggg cggagctgtt cgtccgcgag atcgccggcg
     7081 accacgtccg catcggcgtc accgtccgca acgagagtgg ccagtggagg cgagtggacg
     7141 gcttcatccg cgtcccgcgc ggcagcgacc tcgaggccgc cgatctcgtg atcgagcgcg
     7201 agaacagccg gctcctcacg gtcgagttcg tcgacgtcca gcagggcgac gccgcgatcg
     7261 tcgagtcgcc gtcggggaag gtcgtgctga tcgacggagg cgagagccag ctgttcgcgc
     7321 gctacctcgc gacgaggttc cgggacaccg agccgacgaa gccgaaggag atcgacgaga
     7381 tcgtcgtgac
//
LOCUS       DASQDO010000499         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_725996, whole genome shotgun sequence.
ACCESSION   DASQDO010000499 DASQDO010000000
VERSION     DASQDO010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725996"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VEV43_13715"
     CDS             complement(<1..574)
                     /locus_tag="VEV43_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24622.1"
                     /translation="MIAGGAEPLLAQTPAPAYAYEVVDLGTFGGELANAFSINNRGEV
                     VGRAEDPNRASRGFLWRDGEMIDIGGLSNSGTGTTGFAINDRGEIVGSSLAPDPQFGG
                     RSSMPFYWSEERSQAGSFTREGTIDNPSPLGVGVTRAEFTATCPASPSTQGVDGHRFT
                     LPDVPLRDAVADFTATTGAALRVATWTGDCT"
     gene            813..>3693
                     /locus_tag="VEV43_13720"
     CDS             813..>3693
                     /locus_tag="VEV43_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24623.1"
                     /translation="MSYGIVGRRGGAVVVALVVLAATAVGLPRAAAAPPALPPCPAEY
                     TDNPQGSNWEVTKPDGTTLFLLEAMPCVGDRIGPWKEPVLPNRVELDREMVFEWEASC
                     GTGQGVPCLQFWDTELLLFKKGQNGNLVQVPSNDAEGLYLDIRSHPECDGAGNLQVCQ
                     KMTLRSATGTVPRDLVALVGVTGRSMMYGDNPEMHEYGLPWKWIAVPVVGPGSFKGLD
                     VTLTAYGPGREYSLDRMISIAQTQTVELTLTNEGDEPLTGLSFTGGEPLVVDPRSTGG
                     LEIVSGPVPAIDPAMTLAPGDEKTFTFEVQATAEGIAAAHSQVVAVDPEGNEHKEAHS
                     LKYDIEDGVEMTRNVGQWLRMQAMDQLLQETFGKAYGSMVRRGQEVSARMAELFPPER
                     RRQWFGSETGFPLSPTDFSIALLRGAAPEMIAALLPKEDLDGHTVEELDAAYNAAFKK
                     EVGKGVKKYVQGWADLASATQKFAADSWTESMLTANYVLGSATPDERAQFEAYAMTLI
                     DGTAADTKNLVNDVKREIPKWRENGTYLVEALEETQRGGGIRGAMKTAVKLKEAYDAE
                     SAARDELLKLAETDPLAFQRGWAKRDAQILNKGLVPIFDTLLGGGIVKGAGKLKGIVF
                     RGNGAGIIRAGEASGVLDDGANVAKGAPKIEMGAGSAAPQGMANGEVAELARKGNYLE
                     EVEGATIVQSSDLGNVYELPNLGGVPETTLDAKAAILKSLETEYFEKTGQTLHLAEVL
                     KPSSALRKVGGVAKTELTAAKTGKAAMLDAGAPKDVLAEANVWTGPDPRTLAGFDDLP
                     KLRQQAAIKEWEKAQKSWDAYHNPATGSKEERLRQVVGQEARVPLDLKPHDSGLQRFV
                     TAHYEEVAVTEGQAQAKLIRVKKYEIEVVDTTRNNQVVNRKTVVDLPEAAPQTPDADA
                     VGMAKVVGRDPAGKPILEPLSRAEREFVMQRYIDKNIKARRLAPGT"
BASE COUNT          639 a         1229 c         1331 g          494 t
ORIGIN      
        1 gagtgcagtc ccccgtccac gtcgcgacgc gcagcgccgc gccggtcgta gcggtgaagt
       61 ccgcgaccgc gtctctcagc gggacgtcgg gcagcgtgaa ccggtggccg tcgacgccct
      121 gagtggacgg ggacgccgga cacgtcgccg tgaactccgc gcgcgtcact ccgaccccga
      181 gcgggctggg attgtcgatg gtgccctcac gagtgaacga cccggcctgc gacctctcct
      241 ccgaccagta gaacggcatc gacgaccgcc cgccgaactg cgggtcgggc gcgaggctgc
      301 tcccgacgat ctcgccgcgg tcgttgatcg cgaagccggt cgtgccggtc ccggagttgc
      361 tcaggccccc gatgtcgatc atctctccgt cgcgccacag gaatccccgc gacgcgcggt
      421 tcgggtcttc ggcgcgaccg acgacctcac cccggttgtt gatcgagaac gcgttggcga
      481 gctcgcctcc gaacgtgccg aggtcgacga cctcgtaggc gtaggccggg gcgggtgtct
      541 gggcgaggag cggctcggcg ccgcccgcga tcacgagggc cgccgtagcg acggccaggg
      601 ctatgcgtga gatcggacaa cctcctgtgg ctcgagcccg ccgaggaccg gttcgtcgga
      661 gtacgtccga ttcctccttg cttgctcagg ttcgtcaccg tcgcacggct acgtagtcgg
      721 cgaagcccga accccccgga agggccatgg agcgccgtgg cctcgcccgc catagtcgag
      781 cgggggctgg gccggcgcga ggagggcact ggatgtcgta cgggatcgtc ggtcgccgcg
      841 gcggagcggt cgtggtggcg ctcgtcgtgc tcgcggccac cgccgtaggg ctcccgcggg
      901 ctgctgccgc gcccccggcg ctccccccgt gccccgcgga gtacacggac aacccccagg
      961 gcagcaactg ggaggtcacc aagcccgacg ggacgaccct gttcctcctc gaggcgatgc
     1021 cctgcgtcgg cgaccgcatc gggccgtgga aggagcccgt tctaccgaac agggtcgagc
     1081 tcgacaggga gatggtcttc gagtgggagg cgtcgtgcgg cacgggacag ggggtcccct
     1141 gcctccaatt ctgggacacc gagttgctgc tgttcaagaa gggtcagaac ggcaacctcg
     1201 tgcaggtccc gtcgaacgac gccgagggcc tctacctcga catccggtcg caccccgagt
     1261 gcgacggcgc ggggaacttg caggtctgcc agaagatgac ccttcgttcg gcgaccggca
     1321 cggtcccgcg ggacctggtg gctctcgtcg gcgtcaccgg ccgttcgatg atgtacgggg
     1381 acaaccccga aatgcacgaa tacgggctcc catggaagtg gatcgccgtc ccggtcgtcg
     1441 ggcccggctc gttcaagggc ctcgacgtca cgctcacggc gtacggcccc ggcagggagt
     1501 actcgctcga ccgcatgatc tcgatcgccc agacacagac ggtcgagctg acgctgacga
     1561 acgagggcga cgagccgctg acggggctgt cgttcaccgg cggggagccg ctggtcgtgg
     1621 acccccggag caccgggggc ctcgagatcg tgtcgggccc ggtgcccgcc atcgatccgg
     1681 ccatgacgct cgcgcccggc gacgagaaga cgttcacgtt cgaggtccag gcgacggccg
     1741 agggcatcgc cgcggctcat tcgcaggtcg tggcggtcga tcccgaaggg aacgagcaca
     1801 aagaggcgca ctcgctcaag tacgacatcg aggacggcgt cgagatgacg cgcaacgtcg
     1861 ggcagtggct gcgcatgcag gcgatggacc agctgctgca ggagaccttc ggcaaggcgt
     1921 acggctcgat ggtccgccgg ggacaagagg tctccgctcg catggccgag ctcttcccgc
     1981 cggagcgtcg caggcagtgg ttcggaagcg agacgggctt cccgctgtcg ccgacggact
     2041 tctcgatcgc gctgttgcgc ggcgcggcgc cggagatgat cgcggcgctg ctgccgaagg
     2101 aggacctcga cggccatacg gtcgaagagc tcgacgccgc ctacaacgcc gcgttcaaga
     2161 aagaggtcgg caagggcgtg aagaagtacg tccagggctg ggccgacctc gcgtccgcga
     2221 cgcagaagtt cgcggcggac tcgtggacgg agtcgatgct caccgcgaac tacgtgctgg
     2281 ggagcgcgac acccgacgag cgcgcgcagt tcgaggcgta cgcgatgacg ctgatcgacg
     2341 gcaccgccgc cgacaccaag aacctcgtca acgacgtgaa gcgtgagatc ccgaagtggc
     2401 gcgagaacgg cacgtacctc gtcgaggcgc tggaggagac gcagcgcggc ggcgggatca
     2461 ggggcgcgat gaagacggcg gtgaagctca aggaggctta cgacgcggag tccgcagcgc
     2521 gtgacgagct gctgaagctc gccgagaccg accccctggc cttccagcgg ggctgggcca
     2581 agcgcgacgc gcagatcctc aacaagggcc tcgtcccgat cttcgacacc ctgctcggtg
     2641 ggggcatcgt caagggagcc ggaaagctca aggggatcgt gttccgcggc aacggcgccg
     2701 gcatcatccg cgccggcgag gcgtccggcg tcctcgacga cggcgcgaac gtcgcgaagg
     2761 gagcgccgaa gatcgagatg ggcgccggtt ccgcggcccc ccagggcatg gcgaacggcg
     2821 aggtcgcgga gcttgcacgc aagggcaact acctcgagga ggtcgagggg gccacgatcg
     2881 tccagtcgag cgacctcggc aacgtttacg agctgccgaa tctcggcggc gtccccgaga
     2941 ccacgctcga cgcgaaggcg gcgatcctga agtcattgga gaccgagtac ttcgagaaga
     3001 cggggcagac gctgcacctc gccgaggtcc tgaagccgtc gagcgcgttg cgcaaggtcg
     3061 gtggcgtcgc gaagaccgag ctgaccgcgg cgaagaccgg gaaggccgcg atgctcgacg
     3121 cgggggcacc caaggacgtc ctcgcggagg cgaacgtctg gacgggaccc gacccgcgca
     3181 cgttggccgg cttcgacgac ctgccgaagc tgcgtcagca ggccgcgatc aaggaatggg
     3241 agaaggccca gaagagctgg gacgcgtacc acaaccccgc gacgggctcc aaggaggagc
     3301 gtctgcggca ggtcgtgggc caggaggcgc gcgttccgct cgacctcaaa ccgcacgatt
     3361 caggcctcca gcggttcgtg accgcgcact acgaggaggt cgcggtcacg gagggccagg
     3421 cccaggcgaa gctcatccgc gtgaagaagt acgagatcga ggtcgtcgac acgacgcgca
     3481 acaaccaggt cgtcaaccgc aagacggtcg tcgacctgcc cgaggccgcg ccgcagaccc
     3541 ccgacgcgga cgccgtcggc atggctaagg tcgtcggccg ggaccccgcc gggaagccga
     3601 tcctcgaacc gctgagccgg gccgagcggg agttcgtgat gcagcgctat atcgacaaga
     3661 acatcaaggc ccgccggctc gcgccgggga ccc
//
LOCUS       DASQDO010000500         1834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1154058, whole genome shotgun sequence.
ACCESSION   DASQDO010000500 DASQDO010000000
VERSION     DASQDO010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154058"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VEV43_13725"
     CDS             <1..351
                     /locus_tag="VEV43_13725"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495607.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HYP24624.1"
                     /translation="FPTAFQGTCMHEFVATAKPLKRHGVRAMDVAKRLIDLGYHPPTV
                     YFPLVVEEALMVEPTETESKETIDGLAAALNRIAAEAEAEPDLLHAAPASTPVRRPDE
                     ARAARDLKVRWTPE"
     gene            432..1406
                     /locus_tag="VEV43_13730"
     CDS             432..1406
                     /locus_tag="VEV43_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYP24625.1"
                     /translation="MRTTTSRIALVCALIAGAFVGSVPTSARQSALQPGAQVCDDPDR
                     SVSLFAEPLGDGRVGYGTAPGEASIPGPTLEMVEGECLAVTLTNDAKRRVSIHSHGVD
                     YTVTSDGTPLNDSCVAPGRSKTYFFQAHLPGTRSDGTVDAGSAGYWHYHDHCLGTEHG
                     TGGVYAGLFGAFVVRRAGDPVPDRTCVLVMIKTTFNLRKDPATPRCESNVGDRVEFVV
                     IGHGDTFHTFHLHGHRWTDNRTGMPATVNDATRLIDNKVVGPADSFGFQVIAGEHVGP
                     GPWMYHCHVQGHSDAGMSGIFVVNTPDGRRTPATIEALRELRRGTHSH"
     gene            1387..>1834
                     /locus_tag="VEV43_13735"
     CDS             1387..>1834
                     /locus_tag="VEV43_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP24626.1"
                     /translation="MGRTRTSVAAALLLAGLVVAAPARAADESVTARDNLFASQEIHV
                     DPGDTVTWTNAGRRTHDVTADSGTFVSGDLFPRARFSHTFAKEGTYYYHCSFHGKAGK
                     QGMWGVVVVGDPEPPHGGGGGKDERPRIDVPGDFRTIEGAVDAAEPG"
BASE COUNT          286 a          637 c          659 g          252 t
ORIGIN      
        1 ttcccgaccg cgttccaggg cacgtgcatg cacgagttcg tcgcgacggc caagccgctc
       61 aagcggcacg gggtcagggc gatggacgtg gcgaagaggc tgatcgacct cggctaccac
      121 cctccgaccg tgtacttccc gctcgtcgtc gaagaggccc tgatggtcga gccgaccgag
      181 accgagagca aggagaccat cgacgggctc gccgccgccc tgaaccggat cgccgccgag
      241 gccgaggccg agcccgatct gctccacgcg gcccccgcgt cgaccccggt ccggaggccc
      301 gacgaggccc gcgccgcccg cgacctgaag gtgcgctgga cccccgaata gtcggttcgg
      361 gtggtggttt tcgtcgtacc cccgtgattg catgaccttg cggcacaacc ccccaatcga
      421 aaggccctgc gttgcgtacg accacgtccc ggatcgcgct cgtctgcgca ctgatcgccg
      481 gtgcgttcgt gggatccgtc ccgacgagcg ctcggcagtc cgcgctccag ccgggggcgc
      541 aggtatgtga cgacccggac cgttccgtgt cgctgttcgc cgagccgctc ggtgacggcc
      601 gcgtcggcta cggcaccgcg ccgggcgagg cgagcatccc cgggccgacg ctcgagatgg
      661 tcgagggcga gtgcctcgcg gtcacgctga cgaacgacgc gaagcggcgg gtcagcatcc
      721 actcgcacgg cgtggactac acggtgacca gcgacggcac gccgctgaac gacagctgcg
      781 tcgcgccggg acgcagcaag acgtacttct tccaggcgca tcttccgggc acacgctccg
      841 acggcaccgt cgacgcgggg agcgcggggt actggcacta ccacgaccac tgtctgggga
      901 ccgagcacgg gaccggcggt gtctacgcgg ggctgttcgg ggcgttcgtg gtgcgccgtg
      961 ccggcgaccc cgtccccgac cggacgtgtg tgctcgtgat gatcaagacg acgttcaacc
     1021 tccgcaagga cccggcgacg ccgcgctgcg agtcgaacgt aggggaccgc gtcgagttcg
     1081 tagtgatcgg tcacggcgac acgttccaca cgttccatct gcacgggcac cggtggaccg
     1141 acaacaggac cgggatgccc gcgaccgtga acgacgcgac gaggctcatc gacaacaagg
     1201 tcgtgggccc ggccgactcg ttcgggttcc aggtgatcgc gggcgagcac gtggggccgg
     1261 ggccgtggat gtaccactgc cacgtccagg gccactccga cgccgggatg tcgggcatct
     1321 tcgtcgtgaa cacgcccgac ggccggcgga cgccggcgac gatcgaggcg ctacgggagc
     1381 tgcggcgtgg gacgcactcg cactagcgtc gctgccgcgc tgctgctggc cggcctcgtg
     1441 gtggcggctc ccgcgagggc cgcggacgag tccgtgaccg cacgcgacaa cctgttcgcg
     1501 tctcaggaga tccacgtcga tcccggcgac accgtgacgt ggacgaacgc cgggcggcgc
     1561 acgcacgacg tcaccgcgga ctcggggacg ttcgtgtcgg gcgacctgtt cccgcgggcg
     1621 cgcttcagcc acacgttcgc gaaggagggc acgtactact accactgcag cttccacggg
     1681 aaggcgggca agcaggggat gtggggcgtg gtcgtcgtcg gcgaccccga gcctccgcac
     1741 ggcggcggag gggggaagga cgaacgcccg cgcatcgacg tgccggggga cttccgcacg
     1801 atcgagggcg cggtcgacgc ggccgagccc ggct
//
LOCUS       DASQDO010000501         7061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_236799, whole genome shotgun sequence.
ACCESSION   DASQDO010000501 DASQDO010000000
VERSION     DASQDO010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236799"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VEV43_13740"
     CDS             <1..495
                     /locus_tag="VEV43_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24627.1"
                     /translation="GSVTFCAGWLGKNEDPVLQASGAVVVAGLALLGAAAGLSTWQEE
                     RQRAREQQQREVYAALAFQLLSRFTGVGGSDPQLLAALRTQVTVWAEGPVVEKLGAWN
                     AVYDRYVPDLADGEVFRLSSEAKAEFERATAEVVGAVRKELSPGDDVSVETLTHALFN
                     KPEQ"
     gene            648..1340
                     /locus_tag="VEV43_13745"
     CDS             648..1340
                     /locus_tag="VEV43_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24628.1"
                     /translation="MREQLDRLEAELKRIAGVKNARIVGTEAPEEIHVVATATRSPKQ
                     VVRDVQSLAAAGYGLPIDHRIVSVVQLEDAEPPPPPSTIKLDPPVEEAAPATVGAPAR
                     HEDRRPVLERVVLGTRGSAGWVDVGLKWPDGTETDGSGNAGPSRETRARGAAKALVKA
                     LEPILSERGAELELDQVLIHRMDSADSVFVRATFFDDGHAVPVVGSAIVYDDVASAAV
                     RALLHAVNRKLT"
     gene            1505..1942
                     /locus_tag="VEV43_13750"
     CDS             1505..1942
                     /locus_tag="VEV43_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24629.1"
                     /translation="MKKRFVGLTLVAVFLMAFAGAAIASHLANHTLFHTTTGAKFDGQ
                     WEWFSSSENHGGWHFWGNLVDTICDDDDNVYSKTRAEGYSYSSLYGNEACDTSVTGDT
                     EYQNYEIYAPDQNYTTSANYFACRDRNVYADNCSSDEDNTYTR"
     gene            2122..2754
                     /locus_tag="VEV43_13755"
     CDS             2122..2754
                     /locus_tag="VEV43_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP24630.1"
                     /translation="MALAGASIVLGAGDTIGVTGRSGSGKSSLLYCLAGIVRPDAGRV
                     AYAGRDLSTGDDDELSEIRRRDFGFVFQFGELVPELSLHENVSLPLRLNRVGRAETRE
                     RTLEIVEMLGITDCAHKRPGEVSGGQAQRAAVARALVHRPRVIFADEPTGSLDTENSE
                     KVLGAFIDLARARGTTVVLVTHEPAIAAICDRHVDVVDGRVRDARASVLL"
     gene            2751..5042
                     /locus_tag="VEV43_13760"
     CDS             2751..5042
                     /locus_tag="VEV43_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYP24631.1"
                     /translation="MIRLAVLLVRGGGRRAAFRLALIAIGTAAGIFALLLATTFPALL
                     SQRQDRAEARIANTGAAADDQGWIHFAKRFVGTRPLEIVFVASTPSSPTPPGVDRLPA
                     PGEGVVSPALAAMLDAEPAIASLMPRGVTDLISEDGLVAPDELYAYVGKSRSDLPDVA
                     RPLGGFGGDSNPEVDIAGGDLKIVVLSFLGLVGIPLAAFFAVCARLSAATRDRRVAAL
                     RLIGLDATRTRRVNALEPMAAALAGSLLGLGLFTALNPLLASAGLGGLKWFPEDSRPG
                     AATFLLCALVVPALGGLLSVVSSRNAVTNALSVRRQAPPRARSPWRLLPLMVGLGLLG
                     GLLVIGLTASEVPYSYGDWALLAMLAGAVLTAFGLAFGFSVIPVALARAIARRTDRPW
                     ELLGLRRLEFEPASVTRVVSGLVIVVFAMGFATGLQRDARAANTQVGNQQLYALDVIE
                     VPPEARADLLDPDLADAALVFIDSKADAPALGEGLPPPSERPLRALWGSCAAIETFTE
                     GRVDRCVEGRAYVFARSDASQIKDPDATARFPLEIRRIDAGPSFSRDLPDATLTLSQR
                     DMQTIGDPDLLYPVDGLPGGTVPEAATIQLASGADAASVERVVAGVAAVAPTTTLRFL
                     NEDVEAQARAAVTQKLLHVALMLGVFTGFAAFVVAALDRALERRQNLTALSMAGVSTR
                     TLRGAQMIQVAMPLAVASVVAIVSGKVMEQVTVLVGGYTSSWDWAGTGTAAAFAAVAL
                     VLATGLTGLSVSSRIDVSLIRRE"
     gene            complement(5061..5633)
                     /locus_tag="VEV43_13765"
     CDS             complement(5061..5633)
                     /locus_tag="VEV43_13765"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HYP24632.1"
                     /translation="MLTALVLAVLTGVVARLRGGSVDDLAETKFRWTPLLVAGLVLQV
                     TFLYWEPEWLGDGGGLAIVLLSNLTVAAWLFVNRTLPGLLLAGAGMALNVLVIAANGA
                     MPVLPRSAEKAGVEQSLDDASIKHERLDEDTILPWLGDAIPVPPFKEVLSVGDVVLAL
                     GLCRVVDARMMVHKEPRHRIASSGSGPPEA"
     gene            5668..6576
                     /locus_tag="VEV43_13770"
     CDS             5668..6576
                     /locus_tag="VEV43_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYP24633.1"
                     /translation="MQRVLLIVNARAGSVRARTKEVIAKALSADFKLEVADTLSRNHA
                     TELAADAVDRGFDAVVAFGGDGTVNEAAQTLVGTGVALGVLPGGSTNVMARSLGIPRD
                     PVDATAYVAERLRSGSRRSINVGDFGGRYFLFSAGIGLDAEVVKRVEGDPEAKARSAE
                     WTFVRKAVATGVREYRGKSPWLTVEVEGHEPVRTLFAICCNGRPFTYFKRLPVEVCPR
                     ARLDGGLDLYTLSKVRLGTVPRIAWSIFVTRSHVGWRNAHYFHDLEGFSLSADVPLPV
                     QVDGDYIGEQQEATVRLVPEGLEVLV"
     gene            6623..>7061
                     /locus_tag="VEV43_13775"
     CDS             6623..>7061
                     /locus_tag="VEV43_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HYP24634.1"
                     /translation="MAMPTVEQISAVLGEIQDPELHRGLNELNMVRDVTVSGTEVRTL
                     IALTIPGCPLKDYFYTVIPARLKEEFPEISPVQVDLTSMTEEERAALVGGLLKETPAP
                     FARSDSSTPVIAIGSGKGGVGKSTTTVNIAASLAKLGHSVGLLD"
BASE COUNT         1102 a         2398 c         2422 g         1139 t
ORIGIN      
        1 ggctcggtca ccttttgcgc tgggtggttg ggcaagaacg aagaccctgt cctgcaagct
       61 agcggcgcag tggtggtcgc cggactggcg cttcttggcg ccgcagcggg gctgtctacg
      121 tggcaagagg aacgtcaacg cgcccgtgag cagcagcagc gcgaggtgta tgcggccttg
      181 gcgttccagc ttctatcccg ctttacgggg gtgggcggct cggacccgca gcttttggcc
      241 gcactgcgaa cccaggtcac cgtttgggcg gaggggcccg tcgtcgagaa gctgggggca
      301 tggaacgcgg tctacgaccg gtacgttccc gatcttgccg acggtgaagt gttccgcctt
      361 tcgtctgagg ctaaggccga gtttgagagg gcaactgccg aggtggtcgg ggctgtgcgg
      421 aaggaactgt cgccggggga cgatgtctct gtagagactt tgacgcacgc gctattcaac
      481 aagcctgagc agtaggcgcg cgcgaagatg atctgacggg tttggccgga ttggccggtt
      541 cgccgcttga cggtcggttc gcggcatgcc agattctccc tgaaatacgt acaagacgca
      601 gcgggcgtca ggtagttttt tccagacggg cggtgtactt tcctcgcatg agggagcaat
      661 tggaccggct cgaagctgag ctcaagcgga tcgccggggt gaagaacgcc aggatcgtcg
      721 gcaccgaggc gccggaggag atccacgtcg tcgcgaccgc cacgcgctca cccaagcagg
      781 tcgtccgcga cgtccagtcc ctcgccgccg ccggctacgg cctcccgatc gaccatcgca
      841 tcgtctccgt cgtccagctc gaggacgccg agccgccccc gcccccgtcc acgatcaagc
      901 tcgatccccc cgtcgaagag gccgcgcctg caaccgtcgg cgcgccggcc cgccacgagg
      961 accgcaggcc ggtcttggaa cgcgtcgtgc tcggcacccg cgggagcgcg ggctgggtcg
     1021 atgtcggcct caagtggccg gacgggaccg agaccgacgg cagcgggaac gcgggaccgt
     1081 cgcgcgagac gcgcgcccgc ggtgcggcga aggctctcgt gaaggcgctc gagccgatcc
     1141 tctccgagag gggggccgag ctcgagctcg accaggtcct catccatcgc atggactcgg
     1201 cggactcagt gttcgtacga gcgacgttct tcgacgacgg gcacgcggtg ccggtcgtcg
     1261 gctcggcgat cgtctacgac gacgtcgcct cggccgcagt gcgcgcgttg ctgcacgcgg
     1321 tcaaccgcaa gctcacctag aaccaacgcg cccgttctgc ttcccagggc gggtgactac
     1381 tcgcgctccg cgaacacgcg ccatcaagcg cgcacaacaa ctggtcatta ctgagtgttg
     1441 accccacggt gaacaatcga gtcagatgaa ttagtgctca aagcgggcgc agcgaggggg
     1501 aaacatgaaa aagagattcg tcggtttgac cttggtcgcc gtgttcttga tggcgttcgc
     1561 aggggctgca atcgcaagtc atcttgcgaa ccacaccctg ttccacacga ccaccggcgc
     1621 gaagttcgac gggcaatggg agtggttctc gtcgagcgag aaccacggtg ggtggcactt
     1681 ctggggcaac ctcgtcgaca cgatctgcga cgacgacgac aacgtgtaca gcaagacgcg
     1741 ggccgagggc tactcgtact cgagcctcta cggcaacgag gcatgcgaca cgagcgtcac
     1801 gggcgacacc gaataccaga actacgagat ctatgccccc gaccagaact acacgacaag
     1861 cgccaactac ttcgcctgca gggaccgcaa cgtctacgcg gacaactgct ccagcgacga
     1921 ggacaacacc tacacaaggt gacggttctg tcagagtagt tacgtagcag aaaagaagcg
     1981 gacgacgatc gtaggagtgc cccggctccc ggggcgctcc tacgacttta cgaaggggtt
     2041 gcggtggcga acgaaccgaa agcaactggt ggcgaaagct tgctggtggc cgaagggttg
     2101 catctgacgt acggacccac ggtggcattg gcgggcgctt cgatcgttct cggcgcgggc
     2161 gacacgatcg gcgtgacggg gcgcagcggt agcggcaagt cgtcgcttct gtactgcctc
     2221 gcagggatag tccgtcccga cgcaggacgc gtcgcttatg ccggccgcga cctctcgacc
     2281 ggtgacgacg acgagttgag cgagatcagg cgacgcgact tcggcttcgt cttccagttc
     2341 ggcgagctcg tcccggagtt gtcgttgcac gagaacgtct cgctcccgtt gcggttgaac
     2401 cgagtaggcc gtgcagagac gcgcgagcgc actctggaga tcgtcgagat gctcggcatc
     2461 accgactgtg ctcacaagcg gcccggcgag gtctccggag gtcaggctca gcgcgctgcc
     2521 gtcgcgcgag ccctcgtcca caggccgcgc gtgatattcg ccgacgaacc gaccggttcg
     2581 ctggacacgg agaacagcga gaaagtcctc ggcgcgttca tcgacctggc gcgggcgcgc
     2641 ggaaccacgg tcgtcctcgt gacgcacgag ccggcgatcg ctgcgatctg tgaccgtcac
     2701 gtcgacgtcg tcgacggccg agtacgggac gcacgcgcgt cggtgctctt gtgatcagac
     2761 ttgcggtcct gctcgtccgc gggggcggac gcagagcggc gttccgcctc gccctcatcg
     2821 cgatcgggac ggcggccggg atctttgcgc tcctcctggc gacgacgttc ccggcgcttc
     2881 tctcccagcg tcaggaccgc gcggaggctc gcatcgcgaa caccggcgcc gccgccgacg
     2941 accaggggtg gatccacttc gccaagcggt tcgtcgggac ccgtcctctc gagatcgtgt
     3001 tcgtcgcctc cacaccttcg tcacccacgc ctccaggagt cgacaggctc ccggcccccg
     3061 gagaaggggt cgtctcgcct gccctcgccg cgatgctcga cgccgagccc gcgatcgcca
     3121 gcctgatgcc acggggcgtg accgacctga tctccgaaga cgggttggtc gcacccgacg
     3181 agctgtacgc gtacgtgggg aagtcccgca gcgacctccc ggacgtggcc cgcccgctgg
     3241 gaggcttcgg cggcgactcc aatcccgagg tcgacatcgc gggcggtgat ctcaagatcg
     3301 tggtgctcag cttcctgggg ctcgtcggaa tcccgttggc ggcgttcttc gccgtgtgtg
     3361 cgcgcctctc cgcggcaacc cgcgaccggc gcgtcgccgc gttgcgtctg atcgggctcg
     3421 acgcgacgcg gacccgccga gtgaacgcgc tggagccgat ggcggccgcc ctcgcaggga
     3481 gcctcctggg gttgggcctg ttcacggccc tgaacccgct tctggcgtcc gcggggctcg
     3541 gcggcctgaa atggttcccg gaggactccc ggcccggtgc cgcgacgttc ctcctgtgcg
     3601 cgctggtggt gccggcgctc ggcggcctgc tgagcgtcgt ctcgtcccgg aatgcggtca
     3661 ccaacgccct gtccgtccgg cgtcaggccc cgccgcgcgc tcggtcaccc tggcggttgc
     3721 tccccctgat ggtcgggctc ggcctgctcg gcggattgct cgtcatcggc ctcaccgcgt
     3781 ccgaggttcc gtacagctac ggcgactggg ccttgctcgc gatgctcgca ggtgccgtcc
     3841 tgacggcgtt cgggctcgcg ttcgggttca gcgtcatccc cgtcgcactg gcgcgggcga
     3901 tcgcccgccg gaccgacagg ccgtgggagc tcctcgggct gaggcggctc gagttcgagc
     3961 cggcgagcgt gactcgggtg gtgtcgggtc tcgtcatcgt ggtgttcgcg atgggtttcg
     4021 ccaccggcct ccagcgagat gcccgggccg cgaacacaca ggtcggcaac cagcagctct
     4081 atgcgttgga cgtgatcgag gtacccccgg aggccagggc ggacctgctc gaccccgacc
     4141 tcgcggacgc cgctctcgtg ttcatcgact cgaaggcgga cgctccggct ctcggcgagg
     4201 gtctgccgcc accgtcggag aggccgctgc gtgctctgtg ggggagctgc gcggcgatcg
     4261 aaacgttcac cgagggccgc gtggaccgtt gcgtcgaggg acgggcgtac gtcttcgccc
     4321 ggtccgacgc gagccagatc aaggatcccg atgcgacggc gcgtttcccc ctcgagatca
     4381 ggaggatcga cgccggcccg tcgttctcac gcgacctccc ggacgcgacg ctgacgctgt
     4441 cgcagcggga tatgcagacg atcggtgacc ccgatctcct gtatccggtc gacggccttc
     4501 ccggcggaac cgtgcccgag gccgcgacga tccagctcgc gtcgggcgcc gacgcggcgt
     4561 ccgtggagcg tgtggtcgcg ggcgtcgcgg ccgtcgcccc gacaactacg ctgcgattcc
     4621 tcaacgagga cgtggaggct caagctcgcg cggcggtcac tcagaagctg ttgcacgtcg
     4681 ccctgatgct cggcgtcttc accggattcg ccgccttcgt cgtagcggcg ctggaccgcg
     4741 cactcgaaag acggcagaac ctgacggcgt tgtcgatggc cggcgtctcg acccggaccc
     4801 tcaggggcgc ccagatgatc caggtcgcga tgcccttggc cgtagcgtcg gtcgtggcga
     4861 tcgtctcggg caaggtcatg gaacaagtga ccgtcctggt cggcggatac acgagctcgt
     4921 gggactgggc cggcaccggt acggcggcgg cgttcgcggc cgtcgcgctc gtcctcgcca
     4981 ccggcctgac cggtctgagc gtctcctcgc gcatagacgt ctcgttgatc cgccgggagt
     5041 agcgcccggc gcgacctcgt ctatgcctcg ggcggcccgg agccggagct cgcgatcctg
     5101 tgccggggct ccttgtggac catcatgcgc gcgtccacga cgcggcacag ccctagcgcc
     5161 agcactacgt cgcccacgct cagcacctcc ttgaacggcg gcacggggat cgcgtccccc
     5221 agccacggca ggatcgtgtc ctcgtcgagg cgttcgtgct tgatgctcgc gtcgtccagc
     5281 gactgctcga cacccgcctt ctcggcagag cgcggcagca ccggcatcgc cccgttcgcc
     5341 gcgatcacca acacgttcag cgccatgccg gcccccgcga ggagcagccc cggcagcgtc
     5401 cggttgacga acagccacgc cgctaccgtc aggttcgaca gcagcacgat cgcgaggcct
     5461 ccgccgtcgc cgagccactc gggctcccag tagaggaacg tgacctggag gacgagcccg
     5521 gcgacgagca gcggcgtcca ccggaacttc gtctcggcga ggtcgtccac cgacccgccg
     5581 cggagccgcg cgacgacccc ggtgagcacg gcgaggacca aggcggtcag cacggtcgca
     5641 ggatatggcc ccgatacgat gcggcccgtg caaagggtgc tgctgatcgt caacgcgcgg
     5701 gcgggctcgg tccgggcgcg gacgaaagag gtgatcgcga aggcgctgtc ggcggacttc
     5761 aagctcgagg tagccgacac gctctcgcgc aaccacgcaa ccgagctcgc cgcggacgcg
     5821 gtcgaccgcg ggttcgacgc cgtcgtcgcg ttcgggggag acgggaccgt gaacgaggcc
     5881 gcgcagacgc tcgtaggcac cggagtagcg ctaggcgtcc tgccgggcgg gagcacgaac
     5941 gtcatggcgc ggtcgctcgg catcccgcgc gacccggtcg acgcgaccgc ctacgtagcg
     6001 gagcggctgc ggtccggctc gcgccggtcg atcaacgtcg gcgacttcgg cggccgctac
     6061 ttcctgttct cggcgggcat cggcctcgac gccgaggtcg tgaagcgcgt cgaaggcgat
     6121 cccgaggcca aggcgcgcag cgccgagtgg acgttcgtcc gcaaggcggt cgccaccggc
     6181 gtccgcgaat accgggggaa gagcccgtgg ctgacggtcg aggtcgaggg ccacgagccc
     6241 gtccgcacgt tgttcgccat ctgctgcaac ggccgtccgt tcacgtactt caagcggctg
     6301 ccggtcgagg tgtgcccgcg ggcgcggctc gacgggggcc tcgacctcta cacgctctcg
     6361 aaggtcaggc tcggcaccgt tccccggatc gcgtggtcga tcttcgtcac ccgctcgcac
     6421 gtcgggtggc gcaacgcgca ttacttccac gatctcgagg gcttctcgct gagcgccgac
     6481 gtcccgctgc cggtccaggt cgacggcgac tacatcgggg aacagcagga ggccacggtg
     6541 cgtttggtac ctgagggcct ggaagttctc gtttaaagct aggctccggc gtaatcccct
     6601 gagcgacagg agtagtccca gcatggccat gcccaccgtc gagcagatca gcgccgtcct
     6661 cggagagatc caggaccccg agctccaccg cgggctcaac gagctgaaca tggtgcggga
     6721 cgtcacggtc tccggcacgg aggtccgcac gctgatcgcg ctcaccatcc ccggctgtcc
     6781 cctgaaggac tacttctaca cggtgatccc ggcgcgcctg aaagaggagt tccccgagat
     6841 ctcgcccgtc caggtcgacc tcacgtcgat gaccgaggag gagcgtgcgg ccctcgtcgg
     6901 cgggctcctc aaggagaccc cggccccctt cgcgcgcagc gactcgtcca cgccggtgat
     6961 cgcgatcggc tcgggcaagg gcggcgtcgg caagtcgacg acgacggtga acatcgcggc
     7021 gtcgctcgcg aagctcgggc actccgtcgg gctcctcgac g
//
LOCUS       DASQDO010000502         5781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_726100, whole genome shotgun sequence.
ACCESSION   DASQDO010000502 DASQDO010000000
VERSION     DASQDO010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726100"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..1581
                     /locus_tag="VEV43_13780"
     CDS             418..1581
                     /locus_tag="VEV43_13780"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYP24635.1"
                     /translation="MSSRGDVRSEWVQGHLAEATRIATLLTADPARGPVVAREALDAA
                     LEQVPRRRLDGRLADALLAQLVRHSKAEPEPAPPDLPEQLAALRTLPRRQRAALVLRH
                     YAELSDERAAVFLDCSPKAVADLVGRAVRALPAEARSDLHDWLDAAPLPRAAAARPKR
                     SGVRRVFRPRVVRAIGVTAAVAAGIAGGLRIPALVDEPEPPTRAERLAEIRQVLESRE
                     ASLPFDPDDPGPGASPMFPVVDGVIGGNLWNVMGYRDRTGSSCLQLVVDYDFGRRRCL
                     GPAEAPIRATVDVDRQHGVTFISGMVAPEIAELHFVGPTVSWMDVTIGRENPRAQPTQ
                     PGFFGIALPDELVAVPSRRGARAGGYDVYPARLTGIDGDGRGVAKVTLLLTRT"
     gene            complement(1578..2663)
                     /locus_tag="VEV43_13785"
     CDS             complement(1578..2663)
                     /locus_tag="VEV43_13785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP24636.1"
                     /translation="MKVGLVCPYAWDRFGGVQSHVRSLAGALRSRGHDVRVFAPHVAA
                     DAPAPEGVTLVGRAVPVPANGSVATVAFGPTVGPAVRDALDEFSPDVLHLHEPLIPSI
                     SLLALRSAEVPTVGTFHAAMEWSAGYGLGRAALAPTFRRLDVCTVVSESARALVSRYF
                     PGDYLLTPNGIDAPAFADAEPLRHADGPTVLFLSRLETRKGLEVLVRAMAELGGGAKL
                     VVGGSGPQERTVKALARSLGVDAVWLGRVPDAEVAPTYRGADVYCAPALGGESFGIVL
                     VEAMAAGAPVVCSDLPGFRAVAAGAAFLVPPDAHLRLAAALKRVLEDPDKAESMRAAG
                     REVAGRYDWGTLAPRVEEVYEAAFARG"
     gene            complement(2660..3595)
                     /locus_tag="VEV43_13790"
     CDS             complement(2660..3595)
                     /locus_tag="VEV43_13790"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HYP24637.1"
                     /translation="MARALRRSGGARPAQEGRLLQLAYYGYVAGSRLALALPESWAYG
                     IAHFAGGVAARRSRRRGQVAANLARITGEPRDSERVQRLVVEAYRSYARYWLETFRLV
                     REGREFWLERFGAVHGDRLDDALARGKGAVVLVGHLGNWDAAGAWVGATGRKLVTVAE
                     VLRPRRLFEFFLEHRAKLGMTIYGAEPGVTARLVEEAENGAVVAILGDRDLKGTGLEM
                     DFFGEPATFPVGAASIALRAGVPLLVAGVFERRLPDGRRGWFVDFSEPIDLPEDRDAA
                     VADLTRQAVATLEEFVGRHPEQWHVFQPFWVADRR"
     gene            complement(3573..4133)
                     /locus_tag="VEV43_13795"
     CDS             complement(3573..4133)
                     /locus_tag="VEV43_13795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYP24638.1"
                     /translation="MVARTGLRPNVITVLGLLIQIGVAYLIVDGALVAAGLVAVAAGI
                     ADLLDGAVAKARGLVSRFGALFDSTTDRLSDALFFLPIAWLYGVDPDVAAHDEPWVAA
                     VSLAALVFSFLVSYVKARAESLGFECNVGFAERAERLLLMIAGLVFGIVPAMVVVLAV
                     VTFGTFVQRMAHVYEQARSGGPGPAS"
     gene            4337..>5781
                     /locus_tag="VEV43_13800"
     CDS             4337..>5781
                     /locus_tag="VEV43_13800"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYP24639.1"
                     /translation="MKSYATENIRNIVLVGHGGSGKTSLAEGLLFLSGATTRLGKISE
                     GNTVCDFDEEEIRKGISISTALAPIEWGDCKVNVLDAPGYADFISDMRAAMRAADLAV
                     FVVSAVEGVEVQTEVAWRYADELELPRVVLVNKVDRENSSFRRTLDQMRELFGMGIAP
                     ISLPLGREHDFKGVISVIDTAAFEYDENGKSKEVPIPEDRQARVDEVRTALLDSVAET
                     SDELLERYLEGGEISHEEILHALHDGIDNATFFPVLVGSATKLIGVDRLADVIVAAGP
                     SPLDRPPVEGTNGEAREAKPDAPLSAFVFKTMTDPYVGRVSYFRVVSGVLKGDTTLHN
                     STQNLDERVGHLFTMRGKTQEPVQELVAGDIGAAAKLAHTVTGDTISDKANPVVLPSI
                     EPPEPLLPKAIVPKTKGDEDKLMIGLHKLIEEDPALRLERNDETHQTILWGMGDAHLD
                     VTMERLKRKFGVEVGEEPFKVPYRSTLRGKA"
BASE COUNT          893 a         2126 c         2026 g          736 t
ORIGIN      
        1 ggtacgtaat caaaggaagg cctgaacgag ccctggcaag aacggactgc taggaatttc
       61 gtgtggattc ccggccccgt gcgttaggcc cctgtgctaa cgacgggttg ccctcccctt
      121 gatgagtggg gggagaaggt cgctgccctt ggcgggcggt cgtcggactc tcggtgtgcc
      181 tgccggttcg acggcactgg cacgtcggag cggttgcaaa tcgcagacgt agctgccctt
      241 cgacaccggc tcttacattg tggaacttcg gccacgcgcg caccctcctg ccggacccag
      301 tggtctgagt ggtcgaaatc ccgaccacct cgaccactca gaccacttgg tttcccgcgg
      361 cgtgtgcgaa cggcggcggg ccctgcagtt acgggccggc ccccctagca ttgggacatg
      421 tcttcccggg gcgacgtccg gtccgagtgg gtccagggcc acctcgccga ggcgacccgc
      481 atcgccacgc tcctgaccgc cgacccggcc cgcggccccg tcgtcgctcg ggaggccctc
      541 gacgccgccc tcgagcaggt cccccggcgg cggctcgacg ggcgcctcgc cgacgcgctg
      601 ctggcccagc tcgtgcggca ttcgaaggcc gagcccgagc ccgcccctcc cgacctgccg
      661 gagcagctcg ccgccctccg gacgctcccc cgccgccagc gcgcggccct cgtgctccgt
      721 cactacgcgg agctgtccga cgagagggcc gcggtcttcc tcgactgctc gccgaaggcc
      781 gtcgccgacc tcgtcgggcg agcggtgcgg gcgctgccgg ccgaggcccg gtcggacctg
      841 cacgactggc tcgacgccgc gccgcttccg cgcgcggcgg cggcccggcc caagcgatcg
      901 ggggtccgcc gggtgttccg gccccgggtc gtgcgcgcca tcggcgtgac cgcggccgta
      961 gccgccggga tcgccggggg cctgcgcatc ccggccctcg tcgacgagcc cgagccgccc
     1021 acgagggccg agcggctcgc cgagatccga caggtgctcg agtcgcggga ggcctctctt
     1081 ccgttcgacc cggacgaccc cgggcccggc gcgtcgccga tgttccccgt cgtcgacggc
     1141 gtgatcggcg ggaacctctg gaacgtcatg ggctaccgcg accgcacggg cagctcgtgc
     1201 ctgcagctcg tggtcgatta cgacttcggc cggcggcgct gcctcggtcc cgccgaggcc
     1261 cccatccgcg cgaccgtcga cgtcgatcgc cagcacgggg tcacgttcat ctccgggatg
     1321 gtcgccccgg agatagcgga gctccacttc gtcgggccca cggtgtcgtg gatggacgtg
     1381 acgatcggac gcgagaaccc gcgggcgcag cctacacagc cggggttctt cggcatcgcc
     1441 ctgccggacg agctcgtcgc ggtgccgtcc cgcaggggcg cccgtgcggg cgggtacgac
     1501 gtgtacccgg cgcgcctcac cgggatcgac ggcgacggaa ggggcgtcgc gaaggtcacg
     1561 ctgctgctga cccggaccta gccgcgcgcg aaagctgctt cgtagacctc ttcgacccgc
     1621 ggcgccagcg tcccccagtc gtagcgtccg gccacctcgc ggcccgcggc ccgcatcgac
     1681 tccgccttgt ccgggtcttc cagaacacgc ttgagggccg ccgcgagacg caggtgcgcg
     1741 tcgggcggga cgaggaaggc tgcacctgcg gcgacggctc tgaatcccgg gaggtccgag
     1801 cacacgaccg gcgctcccgc cgccatcgcc tcgacgagca cgatcccgaa cgactcgccg
     1861 ccgagcgcgg gcgcgcaata gacgtccgcg ccgcggtagg tgggcgcgac ctcggcgtcc
     1921 ggcacgcggc cgagccacac ggcgtccacg cccagcgagc gcgcgagcgc cttgaccgtt
     1981 cgctcctggg ggcccgaccc gccgacgacg agcttcgcgc cgcccccgag ctcggccatc
     2041 gcccggacca ggacctcgag acccttgcgc gtctcgagcc gcgacaggaa gaggacggtg
     2101 ggcccgtccg cgtgccgcag cggttcggca tccgcgaacg cgggcgcgtc gatcccgttc
     2161 ggcgtcagga ggtaatcgcc cgggaagtag cgcgagacga gcgcgcgcgc cgactccgag
     2221 acgacggtgc agacgtcgag ccggcggaac gtgggggcca gggcggcgcg gccgaggccg
     2281 tagcccgcgc tccactccat cgccgcgtgg aacgtcccta cggtcgggac ctccgccgac
     2341 ctcagcgcca gcagcgagat gctcggtatc agcggctcgt gcaagtggag gacgtcgggc
     2401 gagaactcgt cgagggcgtc gcgcacggcc ggccccaccg tgggcccgaa cgcgaccgtc
     2461 gctaccgagc cgttcgccgg gacggggacc gcgcggccga cgagcgtgac gccctcgggc
     2521 gcgggcgcgt ccgccgcgac gtgaggagcg aacacgcgga cgtcgtggcc gcgggagcgc
     2581 agcgcgccgg cgagggaccg cacgtgtgac tggacgccgc cgaagcggtc ccacgcgtag
     2641 ggacagacga gcccgacctt catcgccggt ccgccaccca gaaaggctgg aacacgtgcc
     2701 actgctcggg gtggcgcccg acgaactcct cgagcgtcgc cacggcttgc ctggtgaggt
     2761 cggccaccgc ggcgtcccgg tcttcgggca ggtcgatcgg ctccgagaag tcgacgaacc
     2821 agccgcgcct cccgtcgggc aacctccgct cgaagacgcc ggcgacgagc aacgggacgc
     2881 ccgccctgag cgcgatgctt gcggccccca cggggaacgt cgcgggctcg ccgaagaagt
     2941 ccatctcgag gcccgtgccc ttgaggtcgc ggtcgccgag gatcgcaacg accgcgccgt
     3001 tctccgcctc ttcgaccagg cgcgcggtca cgccgggctc ggccccgtag atcgtcatgc
     3061 cgagcttcgc ccggtgctcc aggaagaact cgaacaggcg gcggggccgc agcacctcgg
     3121 cgaccgtgac gagcttgcgc cccgtcgcgc cgacccatgc ccccgcggcg tcccagttcc
     3181 ccaggtggcc gacgaggacg acggcgccct tgccgcgggc gagcgcgtcg tcgagccggt
     3241 cgccgtggac ggcgccgaag cgctcgagcc agaactcacg gccctcgcgc acgagcctga
     3301 aggtctcgag ccagtagcgc gcgtacgaac ggtacgcctc gacgacgagc cgctgcactc
     3361 gttcggagtc gcgcggctcg cccgtgatcc gcgcgaggtt cgcggcgacc tgcccgcgcc
     3421 ggcgcgaccg gcgcgccgcg acgccgccgg cgaagtgcgc gatcccgtac gcccagctct
     3481 ccgggagcgc gagcgcgagc cgcgaccccg cgacgtagcc gtagtacgcg agctggagga
     3541 gccgcccttc ctgcgcgggc cgagccccgc cgctacgacg cagggcccgg gccaccgctg
     3601 cgcgcctgct cgtagacgtg ggccatccgc tgcacgaacg tgccgaacgt gacgacggcc
     3661 agcacgacga ccatcgcggg gacgatgccg aagaccaacc ccgcgatcat gagcaggagc
     3721 cgctcggccc tctccgcgaa gccgacgttg cactcgaagc cgaggctctc cgcacgtgcc
     3781 ttcacgtacg agacgaggaa cgagaacacg agcgcggcca gggacaccgc ggcgacccac
     3841 ggctcgtcgt gcgccgccac gtcgggatcg actccgtaga gccacgcgat cggaaggaag
     3901 aacagcgcgt cggagagccg gtcggtggtc gagtcgaaca gagcgccgaa acgcgacacg
     3961 aggccgcgcg ccttcgcgac ggccccgtcg aggaggtcgg cgatcccggc cgcgacggcg
     4021 accagccccg ccgccacgag agcgccgtcc acgatcaggt aggccacgcc gatctggatc
     4081 agcaggccga ggaccgtgat gacgttgggt ctcaggccgg tccgggcaac caccccgccc
     4141 acggggcggg cgaattggtc ccaagctccc cggatcctgg aattgagcac gggcggcatc
     4201 ctagcgagca cccgtgcacg gacgccccag gggccggttt gaccagggcg gatagcatgg
     4261 cgatcaacca acgaccggtc gcctcggcga gcggccactg accaaaacgt cggaccgatc
     4321 cggggggaac gagcgcgtga agagctacgc cacggagaac atccgcaaca tcgtgttggt
     4381 cggccacggg ggttccggga agacctcgct ggccgagggc ctcctcttcc tttccggcgc
     4441 gacgacccgg ctgggcaaga tctccgaggg caacaccgtc tgcgacttcg acgaggagga
     4501 gatccgcaag ggcatctcga tctcgaccgc gctcgcgccg atcgagtggg gcgactgcaa
     4561 ggtcaacgtc ctcgacgcac ccggctacgc ggacttcatc tccgacatgc gcgccgcgat
     4621 gagagcggcc gacctcgcgg tcttcgtcgt ctccgcggtg gagggcgtcg aggtgcagac
     4681 cgaggtggcg tggcgttacg cggacgagct cgagctcccg cgcgtcgtgc tcgtcaacaa
     4741 ggtcgaccgg gagaactcgt cgttccggcg cacgctcgac cagatgcgcg agctgttcgg
     4801 catggggatc gcgccgatct cgttgccgct cggccgcgag cacgacttca agggcgtcat
     4861 ctcggtgatc gacacggctg cgttcgagta cgacgagaac ggtaagtcga aggaggtccc
     4921 gatccccgag gaccggcagg cgcgcgtcga cgaggtccgc accgcgctgc tcgactccgt
     4981 cgccgagacg agcgacgagc tcctcgagcg ctacctcgaa ggcggcgaga tctcacacga
     5041 agagatcctc cacgcgctgc acgacggcat cgacaacgcg acgttcttcc cggtgctggt
     5101 gggctcggct acgaagctca tcggcgtcga ccgtctcgcc gacgtcatcg tcgcggcggg
     5161 gccgtccccg ctggaccggc ccccggtaga gggcacgaac ggcgaggccc gcgaggcgaa
     5221 gcccgacgcg ccgctgtcgg ccttcgtgtt caagacgatg accgacccct acgtgggccg
     5281 cgtgagctac ttccgggtgg tctcgggcgt gctcaagggc gacaccaccc tgcacaactc
     5341 gacgcagaac ctcgacgagc gcgtcggcca cctcttcacg atgcgcggca agacccagga
     5401 gccggtccag gagctcgtcg caggcgacat cggcgcggcg gcgaagctcg cgcacacggt
     5461 caccggcgac acgatctcgg acaaggccaa ccccgtcgtg ctcccgtcca tcgagccgcc
     5521 cgagcccctg ctgcccaagg cgatcgtccc gaagaccaag ggcgacgagg acaagctgat
     5581 gatcggcctc cacaagctca tcgaggagga cccggcgctg cgcctcgagc gcaacgacga
     5641 gacccaccag acgatcctgt ggggcatggg cgacgcgcac ctcgacgtga cgatggagcg
     5701 gctcaagcgc aagttcggcg tcgaggtcgg cgaggagccg ttcaaggtcc cctaccgctc
     5761 gacgctcagg ggcaaggccg a
//
LOCUS       DASQDO010000503         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_726108, whole genome shotgun sequence.
ACCESSION   DASQDO010000503 DASQDO010000000
VERSION     DASQDO010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726108"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VEV43_13805"
     CDS             <1..1365
                     /locus_tag="VEV43_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYP24640.1"
                     /translation="DESVTARDNLFASQEIRIEPGDTVTWTNAGRRTHDVTADSGTFV
                     SGDLFPRARFSHTFAKEGTYYYHCSFHGKAGKQGMWGVVVVGDPEPVEPDQRPRIDVP
                     ADYKTIQGAVDAAEPGSTIVVAPGTYRGEVVIETDDLILRGVDRYRTVLHGRDERDTG
                     ILVEGAAQVTIANLTVRNFVTDGISFVDSFRYTAKGIEAIDNRMHGIAATRSFEGVMR
                     SSFAWGSGEAGFYVGDCMGCGALLDRVQARSSHTGIAALDATGVTIRDSWVVGNGVGI
                     ALVSAGGAAAPGRGALVVSNYVNGDRAPARPPLRSAETFGIPRGTGIWLAGVANAVVR
                     ANRVHDLSRYGILVSATASGEPPERNVVAGNAIPGAGQGQLAWDGAGSDNCFDGNEFA
                     GVPAPPDIERFTCDSRPFTGEPYQPVIDDLATALPASLALETADPPEPDRPSCQKGKP
                     GCRR"
     gene            1391..>1932
                     /locus_tag="VEV43_13810"
     CDS             1391..>1932
                     /locus_tag="VEV43_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03767 family metallophosphoesterase"
                     /protein_id="HYP24641.1"
                     /translation="MNRGRAFGGMLLVLALAAQAAPLAAADRARTSLTTLDRTIVGKG
                     EKDLAFGPGERFVTRALGWKDPGGTGIPLVGFKQLSDVHVIDEESPARVEWLDGCGTP
                     FNAAYRVQEAMSLQVGDSMLRRLARIRRGPATGVPLSFVVSTGDNIDNNQLNELRWFI
                     SLLDGERVNPGSGGPGYHGYA"
BASE COUNT          280 a          683 c          701 g          268 t
ORIGIN      
        1 gacgagtccg tgaccgcgcg cgacaacctg ttcgcgtccc aggagatccg catcgagccc
       61 ggcgacaccg tcacgtggac gaacgccgga cgccgcacgc acgacgtcac cgcggactcg
      121 gggacgttcg tgtcgggcga cctgttccca cgggcccggt tcagccacac cttcgcgaag
      181 gagggcacct actactacca ctgcagcttc cacgggaagg cgggcaagca ggggatgtgg
      241 ggcgtcgtcg tcgtcggcga ccccgagccc gtcgagcccg accagcggcc gcggatcgac
      301 gtcccggcgg actacaagac gatccagggc gcggtcgacg cagccgagcc gggctcgacg
      361 atcgtcgtcg cgcccgggac ctacaggggc gaggtcgtga tcgagacgga cgacctgatc
      421 ctgcgtggag tcgaccgcta ccggacggtg ctgcacggcc gtgacgaacg cgacaccggg
      481 atcctcgtcg agggggccgc gcaggtgacg atcgcgaacc tgaccgtgcg caacttcgtg
      541 accgacggga tctcgttcgt cgactccttc cgctacaccg cgaaggggat cgaggcgatc
      601 gacaaccgca tgcacggcat cgcggcgacg cgctcgttcg agggcgtgat gcggtcgtcg
      661 ttcgcgtggg ggagcggtga ggccggcttc tacgtcggtg actgcatggg gtgcggggcg
      721 ttgctcgacc gggtccaggc acgctccagc cacaccggga tcgccgcgct cgacgcgacg
      781 ggcgtcacga tccgtgactc gtgggtcgtc gggaacggcg tcggcatcgc gctggtatcc
      841 gctggcggcg ccgcggcccc cggccggggc gcgctcgtgg tgtcgaacta cgtgaacggc
      901 gaccgtgcgc ccgcgcggcc cccgctccgc tcggccgaga ccttcgggat cccgcggggc
      961 acggggatct ggttggccgg cgtcgccaac gcggtcgtac gcgccaaccg ggtgcacgac
     1021 ctgtcgcgct acgggatcct cgtctcggct actgcctccg gcgagcctcc ggagcgcaac
     1081 gtggtggccg gcaacgcgat ccccggcgcc ggccaaggtc agctcgcgtg ggacggggcc
     1141 ggctcggaca actgcttcga cgggaacgag ttcgccgggg tccccgcgcc tccggacatc
     1201 gagcgcttca cctgcgacag ccgtcccttc acgggcgagc cgtaccagcc cgtgatcgac
     1261 gacctggcga cggcgctgcc ggcgagcctg gcgctcgaga ccgccgaccc tcccgagccc
     1321 gaccgcccct cgtgccagaa gggaaaaccc ggctgtcgcc gctgagggcg gggcgtaggc
     1381 tgaccgtcgc atgaacaggg gccgggcgtt cggggggatg ttgctcgtgc tggctctcgc
     1441 cgctcaggcg gcgccgctcg cggccgcgga ccgggcccgg acgtctctca cgacgctcga
     1501 ccggacgatc gtcggcaagg gcgagaagga cctcgccttc gggccgggcg agcgtttcgt
     1561 cacgcgcgcg ctcggatgga aggaccccgg cggcaccggc atcccgctcg tggggttcaa
     1621 gcagctcagc gacgtccacg tgatcgacga ggagtcgccg gcgagggtcg agtggctcga
     1681 cggctgcggc actcccttca acgccgctta ccgcgtgcag gaggcgatgt cgctgcaggt
     1741 cggcgactcg atgctccgga ggctcgcgcg catccgccgc ggcccggcga ccggcgtgcc
     1801 gctctcgttc gtcgtgtcga ccggcgacaa catcgacaac aaccagctca acgagctgcg
     1861 gtggttcatc tcgctgctcg acggcgagcg cgtaaacccc ggctccgggg ggcccggcta
     1921 tcacggctac gc
//
LOCUS       DASQDO010000504         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_665271, whole genome shotgun sequence.
ACCESSION   DASQDO010000504 DASQDO010000000
VERSION     DASQDO010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665271"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VEV43_13815"
     CDS             complement(<1..508)
                     /locus_tag="VEV43_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYP24642.1"
                     /translation="MIPVNSVSLTPEDLANVSEAFETGWISSSGRFIEEFETSWAAYC
                     GRRWGVAVSNGTTALQLAVAALRPEPGAEIVMPSFTIMSCALAALYNDCVPVLVDCDP
                     DTWCLDVDQVADKLSDRTFAVMPVHMYGHPADMDPLIRMAAERGVHVIEDAAQSHGAQ
                     YLAGGSEWT"
     gene            complement(514..927)
                     /locus_tag="VEV43_13820"
     CDS             complement(514..927)
                     /locus_tag="VEV43_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24643.1"
                     /translation="MAAGSSQIETVAHEGRVIGLVVRSGALPPETKFLTSPEDNLQLG
                     YVVYPAGGEIQRHVHLPLERELVGTSEVVIVKQGRCEVDFYSDDKELLTTLEMTEGDV
                     MLLLRGGHGFRMTDDTVLFEVKQGPYTGLAEKERF"
     gene            complement(944..2239)
                     /locus_tag="VEV43_13825"
     CDS             complement(944..2239)
                     /locus_tag="VEV43_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYP24644.1"
                     /translation="MTAPGMRVGVAPVRWPGAGGIFQYSQSILTALSGRSGAEVPVFT
                     DDPGAAASVFGQEGLRFVALPAEPKPRGRRVLDRVVGDGRLRQAWRGFRETRSRARRP
                     EAIDANVVNWKPHLREALASAGVDVVIYAAPSTTSFEARTPYMMAIHDLQHRVQPEFP
                     EVSRGREWETREYTMRNGVRHATRILVDSDVGKEDVLDVYGFTGITEDRVEVLPFVPS
                     PLLHRPGDDEVRRVRAHYGLPSGYLFYPAQFWPHKNHMRIVEAVHRLSRRGVEVPVVF
                     VGSHADPLMEETWRRVERLVRTYGLESQVLFLGYVPDDVISVLYADALALLFPTFFGP
                     TNIPVVEAWSYGLPVVTSDLRGIREHTGDAACLVDPRSVDAIAEGIERVTTDETLRAR
                     LIDAGRTRAGSYGRAEFEARLHAILQRAEPVVAAEGFRG"
     gene            complement(2236..>3256)
                     /locus_tag="VEV43_13830"
     CDS             complement(2236..>3256)
                     /locus_tag="VEV43_13830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYP24645.1"
                     /translation="KGLGSDSTHELSPNVRLHLLRVPRVSGMPVAFLPRLAAVRKYVR
                     ALGCDVVHGQGTEAGYAWMATWLGLPNVITVHGILRLTHAVSRPPMLSVEHVGRWIET
                     ATFKRVSNVIAISDFVEEKVSRLAPRARFFSAGNPIDRRFFEMELDAVPREPTAVYLG
                     RITPEKGLIDLMRAAVILDESGVEATIAVLGRASGRDGLSYEARCKDVVKGLRNVRVE
                     FRGWVPDEEVWDLLRRAACLALPSHDESFSMAVAEAMALGTPAVAYAAGAVPERIDHG
                     LNGALVPDGDVAGLARELRSLLEDPSRAAEWGRAARDKARAWHPDTIAQRTVEAYETI
                     LAPGA"
BASE COUNT          489 a         1194 c         1113 g          460 t
ORIGIN      
        1 gcgtccactc ggaaccgccg gccagatact gcgcgccgtg ggactgcgcc gcgtcctcga
       61 tcacgtggac gccgcgctcc gccgccatcc ggatcagggg atccatgtcc gcggggtgcc
      121 cgtacatgtg gaccggcatc accgcgaagg tccggtcgga gagcttgtcc gcgacctggt
      181 cgacgtcgag gcaccacgtg tccggatcgc agtcgacgag caccggcacg cagtcgttgt
      241 agagcgcggc cagcgcacac gacatgatcg tgaacgacgg catgacgatc tcggcccccg
      301 gctccggacg tagcgcggcg accgcgagct gcagcgccgt cgtcccgttc gagaccgcca
      361 caccccagcg ccggccgcag taggccgccc acgaagtctc gaactcctcg atgaagcgtc
      421 cggacgagga gatccatccc gtctcgaagg cttccgagac gttggcgagg tcctccggcg
      481 tcagcgacac cgagttgacc gggatcaccg aggtcagaac cgctccttct cggccaagcc
      541 cgtgtacggg ccctgcttca cctcgaacag cacggtgtcg tcggtcatcc ggaagccgtg
      601 cccgcccctc aacagcagca tgacgtcgcc ttcggtcatc tcgagcgtcg tcagcagctc
      661 cttgtcgtcg ctgtagaagt ccacctcgca acggccctgc ttgacgatca cgacctcgga
      721 ggtcccgacg agctcgcgct cgagcggcag gtgcacgtgc ctctggatct ctccccccgc
      781 cgggtagacg acgtagccga gctggaggtt gtcctcgggg gaggtcagga acttcgtctc
      841 cggcgggagc gcgcccgaac gcacgacgag gccgatgacc cggccctcgt gcgcgacggt
      901 ctcgatctgc gaagacccgg cagccaccgg gcaaccctaa ctctcagccg cggaagccct
      961 ccgccgcgac gacgggctcg gcgcgctgca ggatggcgtg caggcgtgct tcgaactcgg
     1021 cgcggccgta gctccccgcg cgcgtccgcc cggcgtcgat caggcgggcg cgcagcgtct
     1081 cgtcggtcgt cacccgttcg atgccttccg cgatcgcgtc caccgacctc gggtcgacga
     1141 gacaggcggc gtcgcccgtg tgctcacgga tcccgcgcag gtccgacgtg acgaccggaa
     1201 gcccgtacga ccacgcctcc accacgggga tgttcgtcgg gccgaagaac gtcgggaaga
     1261 ggagcgcgag cgcgtccgcg tagaggaccg agatcacgtc gtccgggacg tagccgagga
     1321 acagcacctg cgactcgagc ccgtacgtcc tcaccagccg ctcgaccctg cgccacgtct
     1381 cctccatcag cgggtccgcg tgcgacccga cgaagacgac ggggacctcc accccgcggc
     1441 gcgacaggcg gtggacggct tcgacgatcc tcatgtggtt cttgtgcggc cagaactgag
     1501 ccggatagaa gaggtacccc gacggcaggc cgtagtgggc gcggaccctt cggacctcgt
     1561 cgtcgcccgg ccggtgcagc agcgggctcg gcacgaacgg cagcacctcg acgcggtcct
     1621 cggtgatccc ggtgaacccg tagacgtcga ggacgtcctc cttgccgacg tcggagtcga
     1681 cgaggatccg cgtcgcgtgg cggacgccgt tgcgcatcgt gtactcccgc gtctcccact
     1741 ccctgccgcg cgagacctcg gggaactccg gctgcacccg gtgctggagg tcgtggatcg
     1801 ccatcatgta gggcgtgcgt gcctcgaacg aggtcgtcga gggggccgcg tagatcacga
     1861 cgtcgacgcc cgcgctcgcg agcgcctccc gaagatgggg cttccagttg acgacgttcg
     1921 cgtcgatggc ctcgggtctc cgggcgcgcg accgtgtctc ccggaaaccc ctccaggctt
     1981 gacgcaggcg gccgtcgccc acgacgcgat ccagcacccg gcggccgcgc ggcttcggct
     2041 ccgcgggcag ggccacgaac ctcagcccct cttgcccgaa gacggacgcg gccgcgccgg
     2101 ggtcgtcggt gaagaccggc acctcggcgc cgctcctccc ggacagggcg gtgaggatcg
     2161 actggctgta ctggaagatg ccgccggcgc ccggccacct cacgggcgcg acgcccacgc
     2221 gcatcccggg cgcggtcacg cgcccggcgc caggatcgtc tcgtacgcct cgacggtccg
     2281 ctgcgcgatc gtgtcggggt gccatgcccg cgccttgtcg cgcgcggcgc ggccccactc
     2341 cgcggcccgg ctcgggtctt cgaggaggct gcggagctcg cgggccagac cggcgacgtc
     2401 gccgtcgggg acgagcgctc cgttgagccc gtggtcgatc ctctccggca ccgcccccgc
     2461 cgcgtacgcg accgcagggg tgccgagggc catggcctcg gcgacggcca tcgagaagct
     2521 ctcgtcgtgc gacggcagcg cgaggcacgc cgcgcgccgc aagaggtccc agacctcctc
     2581 gtcgggcacc cacccccgga actcgacgcg cacgttgcgg agccctttca cgacgtcctt
     2641 gcaacgcgcc tcgtacgaca gcccgtcgcg cccggaggcg cgccccagca ccgcgatcgt
     2701 ggcctcgacc cccgactcgt cgaggatcac ggccgctcgc atcaggtcga tcaggccctt
     2761 ctcgggggtt atgcgcccga ggtacacggc cgtcggctcg cgcggcacgg cgtcgagctc
     2821 catctcgaag aaccgccggt cgatcgggtt gcccgccgag aagaaccggg cgcgcggcgc
     2881 gagacggctc accttctcct cgacgaagtc ggagatcgcg atcacgttcg agacgcgttt
     2941 gaacgtggcc gtctcgatcc accggccgac gtgctcgacg ctcagcatcg ggggccgcga
     3001 gaccgcgtgg gtgaggcgaa ggatcccgtg gacggtgatc acgttcggga gcccgagcca
     3061 ggtcgccatc cacgcatacc cggcttcggt cccctgtccg tgcacgacgt cgcacccgag
     3121 ggcgcgcacg tacttgcgga cggcggcgag gcgcgggagg aatgcgacgg gcatgccgct
     3181 gaccctgggc acgcgaagca ggtggagacg gacgttcggc gacagctcgt gcgtcgagtc
     3241 ggagccgagc cccttc
//
LOCUS       DASQDO010000505         6605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_726146, whole genome shotgun sequence.
ACCESSION   DASQDO010000505 DASQDO010000000
VERSION     DASQDO010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726146"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VEV43_13835"
     CDS             <1..404
                     /locus_tag="VEV43_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYP24646.1"
                     /translation="QSAECVGENDSILDAAKMMSKNDIGAMPICGEDNRLKGMLTDRD
                     IVVKVIAEGRDPSQVRAGELGQGKPVTIGADDSVSLAIKTMADHGVRRLPVIDGHDLI
                     GIVSIGDIAQNAEDDDVGTLLELISSAPPNN"
     gene            complement(466..1479)
                     /locus_tag="VEV43_13840"
     CDS             complement(466..1479)
                     /locus_tag="VEV43_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24647.1"
                     /translation="MDKRAVVGAGVLVAAALAWAAFGTGAPEEARPPAAESGPHLFLS
                     GKYGKVWRVGLDGAVAEQHVRELNPGDPPHHLLARGETLLGWGYETYLLDPALEKEAT
                     ELVDDSLFVVASAREDRVWVATEDEDPTTKGRIAAVREITTGGRVTVGDVRPPNGAWP
                     AAAVSAGLLMHPHGDLVVWDPRTDDVVYRFPARGDLGPTHGDLVAWCELGCSELRVTD
                     VTTGRETVIAPPRGFWKFRGWDGAFSPDGATIAVPVSAKAGGKRVQLALVDVGRASAR
                     AVPDTETDELFNFVAWSDTGSHVFFTGAEGDRRTIFVYDPKTSRVRIVPADVGEFYDA
                     AAI"
     gene            1535..2359
                     /gene="pheA"
                     /locus_tag="VEV43_13845"
     CDS             1535..2359
                     /gene="pheA"
                     /locus_tag="VEV43_13845"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HYP24648.1"
                     /translation="MTTVAYQGEKGAYSEEGALSLFPDASLSPLASIRKVFEAVEVGR
                     VEYGLVPIDNSQAGSINETYDLFLKHGLHLVAETVVRVDHCLLALPGTHTDDLTEVIS
                     HPQAIAQCEEFLSALDVTVRPEYDTAGAAKRVAEASLGSTAAIASRRAGEMYGLEVLA
                     ERIQTYPDNHTRFGVLSRAADPLGTPDKCSLVFGVGHVAGSLYRCLGALAERHLSLTK
                     LESRPRAGRPWEYVFYADVTAAAADPQMVEALAELSEHATFTRLLGSYAAAIPARS"
     gene            2403..3884
                     /locus_tag="VEV43_13850"
     CDS             2403..3884
                     /locus_tag="VEV43_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYP24649.1"
                     /translation="MARIDVLPEVTKFLEGSPKMTIGGQPVDAVSGETFEVIDPSTGG
                     VVTEVPRAGVPDVDAAVAAAREAFEDRRWAGLRPGKRAEVLIKLADLLKRNMNELAQL
                     EAIDSGKPVKVASGEVWAAAECFRYYSGWPTKIYGETNPTDDNMFVYALREPIGVCGA
                     IIPWNFPLIMAAWKLGPALACGNTCVLKPAEQSPLTALRLAQLCLEAGVPEGVVNVIP
                     GFGEEAGQALAQHGDVDKITFTGSTEVGRKILHASEGNLKKVTLELGGKSPNVVFADA
                     DLRRAAKGSMLGVFLNTGQMCTAGTRVLVESSIHDEFVESLVGATKSMKVGPALEEDS
                     VIGPVISSEQLERVTGYIEIGKTEGAEVAIGGGRAEGLGDGYFVEPTVFTGVRNDMRI
                     AQEEIFGPVAGVIEVADVDEAIDVANDTIYGLAAAVWTTDLTKAHRVARGIKAGTVWV
                     NTSGLYDPGVSFGGYKQSGFGRELGVHALEAYTQTKSVWVNLK"
     gene            complement(3898..4440)
                     /locus_tag="VEV43_13855"
     CDS             complement(3898..4440)
                     /locus_tag="VEV43_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24650.1"
                     /translation="MATEILTGALVVITAYYAWQNLRMVQEMRAARVAAIQPRLVPTV
                     EHLPAGHANLRVINVGPGAALDVDIHLKLDPGGPDIHYTTSVIPAGEFHDFMIPSDGG
                     ADSVITQLDVITATWELFRLTGTCRDVSGAPQSVDERLPLREYWARRKAALHVWHDEP
                     LPEIAKRLKNIEKLLKKSVK"
     gene            complement(4455..4853)
                     /locus_tag="VEV43_13860"
     CDS             complement(4455..4853)
                     /locus_tag="VEV43_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HYP24651.1"
                     /translation="MDDKVREFLEHNHAAVMTTIKKDGGPHVARMDVGLVQGKLWSSG
                     TQTRIRTKHVRRDPRAALTVLDFSQPYRWLGLECKVTILEGPDAPELNLELYRTIAKE
                     PDDVDEYLEAMVQEQRLIYEFEIVKSYGMH"
     gene            4902..5192
                     /locus_tag="VEV43_13865"
     CDS             4902..5192
                     /locus_tag="VEV43_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24652.1"
                     /translation="MDVEITDEAVEVLKRSLQLGNVDASSGGVRLRTARALGGGVDVQ
                     VELADGPLEGESVVEKDGIRVFVDPSLFDAVPSPILAVEPQHENVVVRPREA"
     gene            complement(5262..5765)
                     /gene="paaD"
                     /locus_tag="VEV43_13870"
     CDS             complement(5262..5765)
                     /gene="paaD"
                     /locus_tag="VEV43_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HYP24653.1"
                     /translation="MSVSVGTVEERVWDALADVLDPEIPAVSVVDMGMIREVSVEDGV
                     ASVVVLPTFTGCPAVPMIERDVAAAVRSVEGVSDVRVRTSFDPPWTDDRITEDGRRKL
                     KEFGLAPPTGKGPVLITEIGLPAVARCPFCGSANTRAENPFGPTPCRALYYCQACRNP
                     FEQFKPV"
     gene            complement(5762..>6605)
                     /gene="paaC"
                     /locus_tag="VEV43_13875"
     CDS             complement(5762..>6605)
                     /gene="paaC"
                     /locus_tag="VEV43_13875"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HYP24654.1"
                     /translation="VSKALVSLLTALGDDELVLGHRHSEWTGYAPHMEEDVAFSSIAQ
                     DEIGHAAAYDSVAAGVVGGSRDELAFGRDPGDYRNAIVCERPNGDWAYTLARHWLYDY
                     ADDVRLEALEESSHPELASLAHKIRREERYHLIHADTWVKRIVRGPVEGRTKLIDAVS
                     EVYVETLGIFEPIEEEAAAVAEGLLPVPSAELLARFVERTAKALDELGLPTEAQRLSD
                     ETAEFEASSSGDLIAGDRAPVELPASTPSAAMGGRSGRHTDDFKELWDVMTLTYRSHP
                     GASW"
BASE COUNT         1024 a         2262 c         2280 g         1039 t
ORIGIN      
        1 cgcagtcggc cgagtgcgtc ggcgagaacg actcgatcct cgacgcagcc aagatgatgt
       61 cgaagaacga catcggcgcc atgccgatat gcggcgagga caaccggctg aaggggatgc
      121 tcaccgaccg cgacatcgtc gtgaaggtga tcgccgaggg ccgcgacccg tcccaggtgc
      181 gggcgggcga gctcggccag ggcaagccgg tcacgatcgg cgccgacgac tcggtcagcc
      241 tcgcgatcaa gacgatggcc gaccacgggg tccggcgcct gccggtcatc gacggccacg
      301 acctgatcgg gatcgtgagc atcggagaca tcgcccagaa cgccgaggac gacgacgtcg
      361 gcacgctgct cgagctgatc tcgtcggccc cgccgaacaa ctagctcgac tcggtcattc
      421 gaggcccggc tcccgtatgg gggccgggcc tttccgcgcc gggggtcaga tcgcggcggc
      481 gtcgtagaac tcgcccacgt cggcggggac gatgcgcacc cgcgacgtct tcgggtcgta
      541 gacgaagatc gtccgtctgt cgccctcggc gcccgtgaag aagacgtggc tgccggtgtc
      601 gctccacgcg acgaagttga agagctcgtc ggtctcggtg tccggcaccg cccgcgcgct
      661 cgcgcgtccg acgtcgacga gcgccagctg cacgcgcttg ccccctgcct tggcggacac
      721 cggcacggcg atcgtcgcgc cgtcgggcga gaaggcgccg tcccagccgc ggaacttcca
      781 gaagccccgg ggcggggcga tcacggtttc ccttcccgtc gtgacgtcgg tgacgcgtag
      841 ctcggagcac ccgagctcgc accacgcgac gaggtcgccg tgggtgggcc cgaggtcgcc
      901 ccgcgccggg aacctgtaca cgacgtcgtc cgtgcgaggg tcccagacga cgaggtcgcc
      961 gtgcggatgc atcaggagcc cggccgacac ggcggccgcc ggccacgcac cgttcggggg
     1021 ccggacgtcc ccgaccgtca cgcgaccgcc ggtggtgatc tcccgcaccg cggcgatccg
     1081 tcccttggtc gtcgggtcct cgtcctcggt cgcgacccac accctgtcct cgcgcgcgga
     1141 cgccacgacg aagagcgagt cgtccacgag ctcggtcgcc tccttctcca gcgccgggtc
     1201 gagtaggtag gtctcgtaac cccagccgag cagggtctcg ccgcgggcga gcaggtggtg
     1261 tggcggatcg ccggggttca gctcgcgaac gtgctgttcg gcgacggccc cgtcgaggcc
     1321 tacccgccag accttgccgt acttgccgga caggaatagg tggggcccgc tctcggcggc
     1381 cgggggccga gcttcttcgg gcgcgccggt gccgaacgcc gcccacgcca acgccgcggc
     1441 gacgaggacg cccgcaccca cgactgctcg cttgtccact gctatgtatg gacgctccga
     1501 gcggaccccc ggttcgcgcc tactctctgc cgtcatgacg accgtcgcgt accaaggcga
     1561 gaagggcgcc tactcggagg agggggcgct gtcgctgttc cccgatgctt cgctgtcgcc
     1621 cctcgcctcc atccgcaagg tcttcgaggc cgtcgaggtc ggccgggtcg agtacgggct
     1681 cgtcccgatc gacaactcgc aggccggcag catcaacgag acctacgacc tcttcctcaa
     1741 gcacgggctg cacctggtcg cggagaccgt cgtgcgcgtc gaccactgcc tgctcgctct
     1801 ccccggcacg cacaccgacg acctcaccga ggtgatctcg cacccgcagg cgatcgccca
     1861 gtgcgaagag ttcctgagcg cgctcgacgt caccgtccgc ccggagtacg acacggccgg
     1921 agcggcgaag cgggtcgcgg aggcgtcgct cgggagcact gccgcgatcg cttcacggcg
     1981 cgccggagag atgtacgggc tcgaggtgct cgccgaacgc atacagacct atcccgacaa
     2041 ccacactcgc ttcggcgtgc tgtcgcgggc ggccgaccct ctcgggacgc cggacaagtg
     2101 ctcgctcgtc ttcggggtcg ggcacgtcgc cggctcgctc tatcgctgcc tcggcgcgct
     2161 ggcggagcgc cacctgagcc tgacgaagct cgagtcgcgg ccccgtgccg gacgaccgtg
     2221 ggagtacgtc ttctacgccg acgtcacggc cgccgccgca gacccccaga tggtcgaggc
     2281 gctggccgag ctcagcgagc acgcgacgtt cacccgcctg ctcggttcgt acgcggcggc
     2341 gatccccgcc cgctcgtaga ctcgcttact cgactacttc aatcgttccc aggagggttc
     2401 ccatggcgcg catcgacgtt ctgcccgagg tgacgaagtt cctggagggc tccccgaaga
     2461 tgaccatcgg cggccagccc gtcgacgcgg tctcgggcga gacgttcgag gtgatcgacc
     2521 cgtcgaccgg cggcgtcgtc acggaggtac cgcgggccgg ggtgcccgac gtcgacgccg
     2581 cggtggcggc cgccagggag gccttcgagg atcggcgctg ggccggcctc cgtccgggca
     2641 agagggccga ggtcctgatc aagctcgccg accttttgaa gcgcaacatg aacgagctcg
     2701 cgcagctcga ggcgatcgac tcggggaagc cggtgaaggt cgcgtcgggc gaggtgtggg
     2761 ccgcggccga gtgcttccgc tactactcgg ggtggccgac caagatctac ggcgagacca
     2821 acccgaccga cgacaacatg ttcgtctacg cgctgcgcga gccgatcggg gtgtgcgggg
     2881 cgatcatccc gtggaacttc cctctgatca tggcggcgtg gaagctcggc cccgcgctcg
     2941 cgtgcgggaa cacgtgcgtg ctcaagccgg ccgagcagtc gccgttgacg gcgctccgtc
     3001 tcgcgcagct gtgcctcgag gcgggcgtgc ccgagggagt ggtcaacgtc atcccgggct
     3061 tcggcgagga ggcgggccag gccctcgcgc aacacggtga cgtggacaag atcacgttca
     3121 cgggctcgac cgaggtcggc cgcaagatcc tccacgccag cgagggcaac ctcaagaagg
     3181 tgacgctcga gctcggcggc aagtcgccga acgtcgtgtt cgccgacgcg gacctgcgtc
     3241 gcgccgcgaa gggctcgatg ctcggcgtgt tcctgaacac gggccagatg tgcacggcgg
     3301 gtactcgcgt gctcgtcgag agctcgatcc acgacgagtt cgtcgagtcg ctcgtcggcg
     3361 ccacgaagtc gatgaaggtc ggccccgcgc tcgaggagga cagcgtcatc ggcccggtca
     3421 tctcttccga gcagctcgag agggtcacgg gctacatcga gatcggcaag acggaggggg
     3481 ccgaggtcgc catcgggggg ggccgcgccg agggcctggg cgacggctac ttcgtggagc
     3541 cgaccgtgtt caccggcgtg cgcaacgaca tgcggatcgc gcaggaggag atcttcggcc
     3601 cggtcgcggg cgtgatcgag gtcgccgacg tcgacgaggc gatagacgtg gcgaacgaca
     3661 cgatctacgg cctggccgcg gcggtgtgga cgaccgacct gacgaaggcg caccgcgtgg
     3721 cccgcggcat caaggccggg acggtctggg tcaacacgtc ggggctgtac gacccggggg
     3781 tctcgttcgg gggctacaag cagtcgggct tcgggcggga gctgggcgta cacgcgctgg
     3841 aggcctacac gcagacgaag agcgtctggg tcaacctgaa gtagggggcg gaccccgcta
     3901 cttcacgctc ttcttcagta gcttttcaat attcttcagt cgtttcgcga tctccggaag
     3961 gggctcgtcg tgccacacgt ggagggcagc cttcctccgc gcccagtact cgcgcagtgg
     4021 gaggcgctcg tcaacggatt gtggtgcgcc cgagacgtca cggcaggtgc cagtgaggcg
     4081 gaataactcc cacgttgcgg taatgacatc aagttgcgtg atcacggagt ccgctccgcc
     4141 gtcgcttggg atcatgaaat cgtgaaattc accggccgga attaccgagg ttgtgtagtg
     4201 gatgtcaggg ccgcccgggt cgagcttcag gtggatatcc acgtcgaggg cggcgccagg
     4261 ccccacattg ataacgcgga ggtttgcgtg gccggccgga aggtgttcaa cggtagggac
     4321 gagtcgtggc tgaattgctg ccactcgtgc tgcgcgcatc tcttggacca tgcgaaggtt
     4381 ctgccacgcg tagtaagcgg tgatcacaac gagcgcgccg gtcagaatct cggttgccat
     4441 cccaactccg ttcgttagtg catcccgtag gacttcacta tctcgaactc gtagatcaga
     4501 cgctgttcct gcaccatcgc ttccaggtac tcgtccacgt cgtccggctc cttggctatc
     4561 gtccggtaca gctccaggtt cagctcgggc gcgtcggggc cctcgaggat cgtcaccttg
     4621 cattcgaggc ccagccagcg gtagggctgc gagaagtcga ggaccgtcag cgccgcgcgc
     4681 gggtcgcggc ggacgtgctt cgtgcggatc ctcgtctgcg tgccggagct ccacagcttc
     4741 ccctgcacga ggccgacgtc catgcgggcg acgtgcggcc ccccgtcctt cttgatcgtc
     4801 gtcatcacgg cggcgtggtt gtgctcgagg aactcgcgca ccttgtcgtc catcgtcggg
     4861 ctccttgtct agtctcgggg gccgagcata ggaggggtgc catggacgtc gagatcaccg
     4921 acgaggccgt cgaggtactg aagagatcgt tgcagctcgg caacgtcgac gcgtcgagcg
     4981 ggggagtgcg gctgcgaacg gcccgcgccc tcggtggggg agtcgacgtc caggtcgagc
     5041 tggcggacgg cccgttggaa ggcgagtccg tcgtagagaa ggacgggata cgggtcttcg
     5101 tagacccgag cctcttcgac gcggtgccca gcccgatcct cgcagtcgag cctcagcacg
     5161 agaacgtcgt cgtccgtccg cgcgaggcct agccggggca ccccaccggt gaggaaaaat
     5221 ccccgctatc cgtccatttt tcctcgctcg tcgcgaggtg actagaccgg tttgaactgc
     5281 tcgaacgggt tccggcacgc ctggcagtag tacagggccc ggcacggcgt cggcccgaac
     5341 gggttctcgg ccctcgtgtt cgcgctcccg cagaaggggc agcgcgcgac cgcgggcagg
     5401 ccgatctcgg tgatcagtac cggccccttg cccgtcgggg gggccaggcc gaactccttc
     5461 agcttgcggc ggccgtcctc ggtgatgcgg tcgtccgtcc acggagggtc gaacgacgtc
     5521 cgcacccgca cgtccgacac gccttcgacg gaccggaccg ccgcggcgac gtcgcgctcg
     5581 atcatcggga ccgccggaca gcccgtgaac gtcggcagca cgacgaccga cgcaacgccg
     5641 tcctcgaccg acacctcgcg gatcatgccc atgtcgacca cggagaccgc ggggatctcg
     5701 gggtcgagga cgtccgccag ggcgtcccac acccgttcct cgacggtgcc gaccgagaca
     5761 ctcaccacga cgctccggga tgcgaccggt acgtcagcgt catgacgtcc cacagctcct
     5821 tgaagtcgtc ggtgtgcctg cccgaccggc cccccatcgc ggcggacgga gtagacgcgg
     5881 gcagctcgac cggcgcgcgg tcgcccgcga tcaggtcgcc cgaagacgac gcctcgaact
     5941 cggccgtctc gtcggagagc cgctgcgctt cggtagggag cccgagctcg tccagcgcct
     6001 tcgcggtccg ctcgacgaac ctcgccagca gctccgccga cggcaccggg agcaaccctt
     6061 cggccaccgc tgcggcctcc tcctcgatgg gctcgaatat cccgagcgtc tcgacgtaga
     6121 cctcggagac cgcgtcgatc agcttcgtcc gcccctctac cgggccgcgc acgatccgct
     6181 tgacccacgt gtcggcgtgg atgaggtggt agcgctcctc gcgcctgatc ttgtgcgcga
     6241 gcgacgccag ctccggatgc gacgactcct cgagcgcttc gagccggacg tcgtccgcgt
     6301 agtcgtacag ccagtgccgg gccagcgtgt aggcccaatc tccgttgggg cgttcgcaga
     6361 cgatggcgtt tcgatagtcg ccggggtcgc gtccgaaggc gagctcgtcg cgcgagcccc
     6421 cgacgactcc ggcggcgacc gagtcgtagg ccgcggcgtg gcctatctcg tcctgcgcga
     6481 tcgacgagaa cgccacgtcc tcctccatgt gcggcgcata gccggtccac tcggagtgac
     6541 ggtgaccgag cacgagctcg tcgtcgccga gcgcggtcag caaggagacg agggccttgc
     6601 tcacg
//
LOCUS       DASQDO010000506         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_665359, whole genome shotgun sequence.
ACCESSION   DASQDO010000506 DASQDO010000000
VERSION     DASQDO010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665359"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..2201
                     /locus_tag="VEV43_13880"
     CDS             249..2201
                     /locus_tag="VEV43_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HYP24655.1"
                     /translation="MRRFAALIALALVAALVPAAPALAERTVTLTGGGWGHGIGMSQY
                     GTLGRAQRGDGHEEILEHYYTGAKVATVGLPGHIRVGLLWGQPAIAVTAEAHRDGGGK
                     AVFKVEGSKAVIAEGGNGTTFKVEASSTGGMRLYRNGERVKHEGRTVFGSPSKKLVLL
                     YEKHGTRIDPAAKPYDYAYGRLDFGTYKTGDCSEGYCTRMVAQLSMQKYLYGLGEVPA
                     SWPHAALRTQVVAARTYAWAKLKATPGQDRYPCDCAVYDSTADQVYSGDGKRLGSGQW
                     WDDWQGAVNATKDEVVLFGGEPITAYYSSSSGGHTENIEKVWGGATPVPYLRGVPDKP
                     DAVDSNPNHSWDPVKMTWGEFGRKLDNLYGVGLPLEDFAVVGPRGVSGRVTPVNETTN
                     RGGVRIRGADKTVRVDGWEFRTDFGLRDTLFYVKIETEVGDNMKRKYRTLDGAPGEPR
                     TEPYAVPRGWKKPRGWAQDFEVGRMWWNRSIRRALWQWGAVLTAYDELGREKSGLGMP
                     TSDVWGPGRFRGASYEKGDILWSKKTGGRAVRGAFRTAYRRNDGAKGPLGLPVGRQTK
                     AKSLPGGGRRQRFQHGSLYLNPKADLVLGLWGRRAERYRKIGEASSDCGYPTEPLTAT
                     ANAATATFEHGVITWTEASGVKVACG"
     gene            2231..2695
                     /locus_tag="VEV43_13885"
     CDS             2231..2695
                     /locus_tag="VEV43_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYP24656.1"
                     /translation="MALEVPPPEAIPVVAAPESRTSGIISIVLVVVSVGFAVAGQLTL
                     KAAMDHIGRIGFEQVSEPAQLIGKAVREPRLWAGLFLFGISSLFWLVVLSRVPLSVAY
                     PFVGISYIFVVAFARFVLDENVPASRWLGVAVVALGIAIIGLSFRRVSGDPL"
     gene            2703..3695
                     /locus_tag="VEV43_13890"
     CDS             2703..3695
                     /locus_tag="VEV43_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYP24657.1"
                     /translation="MKALLLAGGFGTRLRPLTLTRPKPLLPVYNRPHIAHVFDLLQRH
                     GVDEAVLLTSYLASTFDYLVEEAAEAGMVLEVSQEKEPLGTAGALKHAEAFVGGETFL
                     VFNADVLTTADVGALVDFHRAKGAEGTILLTPVEDPSMYGVVPTDDDGRVTAFVEKPP
                     PGEAPTNLINAGVYVLEPSVLDRIPAGEPWSIERATFPQLVASGAGLYALRSDSYWID
                     IGTPEKYLQANLDALHGRVDLLGTLNDDSHVAADARVSFSCLGYGVSVRSGARVSDSV
                     LLPGVTVGEGAEVHRSILGEGVQVAPGAVVEGRTVGDHEIAPAGSAGGTEEVEG"
     gene            3699..>3902
                     /locus_tag="VEV43_13895"
     CDS             3699..>3902
                     /locus_tag="VEV43_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP24658.1"
                     /translation="MRVLVTGAAGFIGSHLAEALRDAGHDVVGVDSFTDYYDPGLKEE
                     NVRGLEVVRADLADDALDELVAEA"
BASE COUNT          581 a         1346 c         1401 g          574 t
ORIGIN      
        1 ctggctcgtc gagccccgcg acgtcgacgg gttcgcggat gcgatccgga cgctggccgg
       61 cgatgccgcg ctgcgccgtt cgtacgtggt cacgggcaag gcccacgccg cgggctacac
      121 ctgggcccgc acggccgcga ccactatctc cgcctaccgt tcagtcttgt agggctgcaa
      181 gccgaagaac actcgtctac gcagatctac ggatggtcgt tatgtcgggt tcgagacaac
      241 atggcgcaat gcgccgtttc gccgctttga tagcgctcgc cctcgtggcg gcgctcgtac
      301 cggcagcgcc cgcgctcgca gaacgaacgg tcacgctgac cggcggcggg tgggggcacg
      361 ggatcgggat gtcccagtac gggacgctcg gccgcgccca gcgcggtgac gggcacgaag
      421 agatcctcga gcactactac acgggcgcga aggtcgccac ggtcggcctc cccggccaca
      481 tccgcgtggg cctgctgtgg ggacagccgg cgatcgcggt cactgccgag gcgcaccgcg
      541 acggcggcgg caaggccgtg ttcaaggtcg aaggcagcaa ggccgtgatc gccgagggcg
      601 ggaacggcac gacgttcaag gtcgaggcca gctcgacggg cggcatgcgg ctctaccgca
      661 acggggagcg cgtgaagcac gagggccgga cggtcttcgg gagcccgtcc aagaagctcg
      721 tccttctcta cgagaagcac ggcacgcgga tcgacccggc ggcgaagccc tacgactacg
      781 cctacggccg gctcgacttc gggacgtaca agaccggcga ctgctccgag ggctactgca
      841 cgcgcatggt cgcccagctg tcgatgcaga agtacctcta cgggctcggc gaggtccccg
      901 cgtcgtggcc tcacgccgcg ctgcggacgc aggtcgtcgc cgcgcggacg tacgcgtggg
      961 ccaagctgaa ggccacgccc ggccaggacc gctatccctg cgactgcgcg gtctatgact
     1021 cgacggccga ccaggtctac agcggtgacg gcaagcggct cggctcgggc cagtggtggg
     1081 acgactggca gggcgccgtg aacgccacga aggacgaggt cgtgctgttc ggcggcgagc
     1141 cgatcaccgc gtactactcg tcctcgtcgg gcggccacac cgagaacatc gagaaggtgt
     1201 ggggcggcgc cacgccggtg ccttacctcc gaggcgtgcc ggacaagccg gacgcggtcg
     1261 actcgaaccc gaaccactcc tgggaccccg tgaagatgac gtggggtgag ttcgggcgga
     1321 agctcgacaa cctctacggc gtcggcctgc ccttggagga cttcgcggtc gtcggccccc
     1381 gcggcgtctc gggccgcgtc acgccggtga acgagacgac gaaccgcggc ggcgtccgca
     1441 tccggggcgc ggacaagacc gtgcgcgtcg acgggtggga gttccggacc gacttcggcc
     1501 tgcgcgacac gctcttctac gtgaagatcg agaccgaggt cggcgacaac atgaagcgca
     1561 agtaccgaac cctggacggc gcgccgggcg agcccaggac cgagccctat gcggtgccgc
     1621 gcgggtggaa gaagccgcgc ggctgggccc aggacttcga ggtcggccgc atgtggtgga
     1681 acaggtctat tcggcgcgcc ctgtggcagt ggggcgcggt gctgacggcc tacgacgagc
     1741 tcggccgcga gaagtccggc ctcggcatgc ctacgtccga cgtctggggg ccggggcgct
     1801 tccgcggggc gtcgtacgag aagggcgaca tcctgtggtc gaagaagacc gggggccggg
     1861 ccgtgcgcgg ggccttccgg acggcgtatc gccgcaacga cggcgcgaag ggccccctcg
     1921 gcctgccggt cggacgacag acgaaggcca agtcgctccc cggcggcggc cgtcgccagc
     1981 gcttccagca cggctcgctg tatctcaacc cgaaggcgga tctggtgctg gggctgtggg
     2041 gccgcagagc cgagcgctac cgcaagatcg gcgaggcgag ctcggactgc ggctatccga
     2101 cggagcccct tacggcgacg gcgaacgcgg cgacggcgac gttcgagcac ggcgtgatca
     2161 cgtggacgga ggcgtccggc gtgaaggtcg cctgcggcta gccgccgtaa cgtcggccta
     2221 gactgcccgc atggctctag aggtaccgcc ccccgaagcg atccccgtcg tagccgcgcc
     2281 cgagtcgcgc acgtcgggca tcatctcgat cgtcctcgtc gtcgtctcgg tcgggttcgc
     2341 ggtcgccggg cagctcacgt tgaaggccgc gatggaccac atcgggcgca tcggcttcga
     2401 gcaggtctcc gagcccgcac agctgatcgg caaggcggtc cgcgagcccc ggctgtgggc
     2461 cggtctgttc ctgttcggga tctcgtcttt gttctggctc gtcgtgctct cgcgggtgcc
     2521 gctctcggtc gcctacccgt tcgtcggcat ctcgtacatc ttcgtcgtcg cattcgcccg
     2581 cttcgtcctc gacgagaacg tccccgcgtc gcgctggctc ggcgtcgcgg tcgtcgcgct
     2641 gggcatcgcg atcatcggac tgtcgttccg ccgggtctcc ggcgacccgc tctaagggct
     2701 cgatgaaggc cctgctgctc gccggcggct tcggcacgcg cctgcgcccc ctgacgctga
     2761 cccggccgaa gccgctgctg cccgtctaca accggcccca catcgcgcac gtcttcgacc
     2821 tgctccagcg tcacggcgtc gacgaggcgg tgctgctcac ctcttatctc gcgtcgacct
     2881 tcgactacct cgtcgaggag gcggccgagg ccggcatggt cctcgaggtg agccaggaga
     2941 aggagccgct cggcaccgcc ggggcgctca agcacgcgga ggccttcgtg ggcggcgaga
     3001 cgttcctggt cttcaacgcc gacgtcctga ccacggcgga cgtcggggcg ctcgtcgact
     3061 tccaccgcgc gaagggggcc gagggcacga tcctgctcac ccccgtcgaa gacccgtcga
     3121 tgtacggcgt ggtgccgacg gacgacgacg gccgcgtgac cgcgttcgtc gagaagcccc
     3181 cgcccggcga ggcccccacg aacctgatca acgcgggcgt ctacgtcctc gagccgtcgg
     3241 tgctcgaccg gatccccgcg ggcgagccat ggtcgatcga acgggccacg ttcccgcagc
     3301 tcgtggcgag cggggcaggt ctctacgcgt tgcgcagcga ctcgtactgg atcgacatcg
     3361 ggacgcccga gaagtacctg caggcgaacc tcgacgcgct ccacggccgc gtcgacctgc
     3421 tcgggacgct caacgacgac tcgcacgtgg ccgcggacgc ccgggtatcc ttctcctgcc
     3481 tgggctacgg cgtcagcgtg cggtccgggg cccgcgtcag cgattccgtg ctcctgcccg
     3541 gcgtcaccgt gggcgagggc gccgaggtcc accgctcgat cctcggggaa ggcgtgcagg
     3601 tggcgcccgg cgcggtggtc gagggccgga ccgtaggaga tcacgagatc gcgccggcgg
     3661 gctcggccgg cggcacagag gaagtggagg ggtaaatcgt gcgcgttctc gtcaccggag
     3721 ccgcgggctt catcggctcc catctcgccg aggcgttgcg cgacgcgggc cacgacgtcg
     3781 tcggcgtcga cagcttcacc gactactacg accccgggct caaggaggag aacgttcgcg
     3841 ggctcgaggt cgtgcgcgcg gaccttgcgg acgacgcgct cgacgaactc gtcgcggaag
     3901 cg
//
LOCUS       DASQDO010000507         1999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1154360, whole genome shotgun sequence.
ACCESSION   DASQDO010000507 DASQDO010000000
VERSION     DASQDO010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154360"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VEV43_13900"
     CDS             complement(<1..946)
                     /locus_tag="VEV43_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYP24659.1"
                     /translation="MNRLLLTTLVLALLAASSAGAADVKPLAGGSPAPDFKLPGVDGK
                     DHTLSDYKDAKILIVLFTCNHCPTSQAYEDRIIRLHEDYQPKGVALVAISPNDPKAVR
                     LDELGYTDLNDTLEEMKVRAADKRFEFPYLYDGETQAASKAYGAIATPHAFVFDGERK
                     LRYQGRIDDAERDPAAAKSHDLREAIDALLAGKLPPKASTNVFGCSTKWADKRDDNER
                     AVERWDREEVTLSTVDADAVKQLVKAEGDKAPLRLVNVWATWCAPCIAEMPELLAIHR
                     MYRKRAFELVTISIDDAADRDAALGMLKENRLSATNHLF"
     gene            1057..>1999
                     /gene="galE"
                     /locus_tag="VEV43_13905"
     CDS             1057..>1999
                     /gene="galE"
                     /locus_tag="VEV43_13905"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HYP24660.1"
                     /translation="MNVLVAGGAGYIGSHTVKRLKEAGHNPVIYDNLSRGHRTVVDIL
                     KVPAVAADLNDRETLVRTLGEHKIDTVMHFAAFAYVGESVDKPLMYYQNNVATTVSVL
                     QAMQDAGVNRFVFSSTCAVYGDPPKVPITEDMPKGPVSPYGRSKLLVEQILQDVPLGW
                     KDFKFAALRYFNASGCAMDGSIGEDHDPETHLIPVVLQSVLGVRPGITVFGTDYPTKD
                     GTNVRDYIHVDDLADAHIAAAGKLDEAKQIFCNLGTGHGFSVKEIIATAEKVAGKKVP
                     VTYGPRRAGDAIALYADPSRARQVLGWEAKHKDPETII"
BASE COUNT          323 a          698 c          662 g          316 t
ORIGIN      
        1 cgaagaggtg gttggtcgcg gagaggcggt tctccttgag catgccgagg gccgcgtcgc
       61 ggtcggccgc gtcgtcgatg ctgatcgtga cgagctcgaa cgcgcgcttg cggtacatgc
      121 ggtggatcgc cagcagctcc ggcatttccg cgatgcacgg ggcgcaccac gtcgcccaca
      181 cgttcacgag ccgcaacggc gccttgtcgc cctccgcctt cacgagctgc ttcaccgcgt
      241 cggcgtcgac cgtcgacagc gtgacctctt cccggtccca cctctcgacg gcccgctcgt
      301 tgtcgtcgcg cttgtcggcc cacttcgtcg agcagccgaa cacgttggtc gacgccttcg
      361 gcggcagctt gcccgccagc agcgcgtcga tcgcctcgcg caggtcgtgg ctcttcgcgg
      421 cggccgggtc gcgctcggcg tcgtcgatgc ggccctggta gcgcagcttg cgttcgccgt
      481 cgaacacgaa cgcgtgcggc gtcgcgatcg cgccgtacgc cttcgaagcc gcctgcgtct
      541 ccccgtcgta gaggtacggg aactcgaacc gcttgtccgc cgcccggacc ttcatctcct
      601 cgagcgtatc gttgaggtcc gtgtaaccca gttcgtccag ccggacggcc ttgggatcgt
      661 tggggctgat ggcgacgagg gcgacgccct tgggctggta gtcctcgtgc agccggatga
      721 tccggtcctc gtacgcctgc gaggtcgggc agtggttgca cgtgaagagc acgatgagga
      781 tcttggcgtc cttgtagtca gacagcgtgt gatcctttcc gtccaccccc ggcagcttga
      841 agtcgggcgc gggcgagcct cccgcaaggg gcttcacgtc cgcggcgccg gccgacgacg
      901 cggcgagcag ggcgagcacg agggtcgtga gtaacagccg gttcatcgag tgctccttcc
      961 gccccgccgg cgaccggaat gcgccgttaa cgctcccggc ccgcatctcc tatactcccg
     1021 cgtcgcgcag gaacacaagg acgaaggagc agtcgcatga acgtgctggt cgcgggcggg
     1081 gcggggtaca tcgggtcgca cacggtcaag cgcctcaagg aggccggcca caaccccgtc
     1141 atctacgaca acctctcgcg cgggcaccgc acggtcgtcg acatcctcaa ggtccccgcc
     1201 gtcgccgccg acctcaacga ccgcgagacg ctcgtccgca cgctcggcga gcacaagatc
     1261 gacaccgtca tgcacttcgc cgccttcgcc tacgtgggcg agagcgtcga caagccgctg
     1321 atgtattacc agaacaacgt ggccacgacg gtgagcgtgc tccaggcaat gcaggacgcg
     1381 ggggtgaacc ggttcgtgtt ctccagcacg tgcgcggtct acggcgaccc gccgaaggtg
     1441 ccgatcaccg aggacatgcc caagggcccg gtcagcccgt acgggcggag caagctgctc
     1501 gtcgagcaga tcctccagga cgtgccgctg ggctggaagg acttcaagtt cgcagccctg
     1561 cgctacttca acgccagcgg ctgcgcgatg gacgggtcga tcggggagga ccacgacccc
     1621 gagacccacc tgatcccggt cgtgctccag tcggtgctcg gcgtccgccc gggcatcacg
     1681 gtgttcggca ccgactaccc gacgaaggac ggcaccaacg tccgcgacta catccacgtc
     1741 gacgacctcg ccgacgccca catcgccgcc gccggcaagc tcgacgaggc caagcagatt
     1801 ttctgcaacc tcggcaccgg ccacgggttt tcggtgaagg agatcatcgc gacggcggag
     1861 aaagtggcgg gcaagaaggt ccccgtgacc tacggccccc gccgggccgg cgacgcgatc
     1921 gccctgtacg ccgatccgag ccgggccagg caggtgctcg ggtgggaggc gaagcacaag
     1981 gaccccgaga cgatcatcc
//
LOCUS       DASQDO010000508         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1093838, whole genome shotgun sequence.
ACCESSION   DASQDO010000508 DASQDO010000000
VERSION     DASQDO010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093838"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VEV43_13910"
     CDS             complement(<1..244)
                     /locus_tag="VEV43_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004057116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HYP24661.1"
                     /translation="MSEQEVVAATDRPATVSSLVRDLRALGVAPGMTLFVHSSLKSLG
                     WVAGAAQAVVLALQEAVGSAGTLALPAHSTSLSEPSE"
     gene            262..1686
                     /locus_tag="VEV43_13915"
     CDS             262..1686
                     /locus_tag="VEV43_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HYP24662.1"
                     /translation="MSAPGTLRYGTTAGRWVVGATVLGSGIAFLDGTVVNVALPAIDA
                     DLGAGLSGLQWTVDAYLLTLSAFLLLGGSLGDLFGRRRMFVYGLGGFAAASLLCAVAP
                     GTEALVAARALQGVAAALLVPGSLAIISTSFAPEDAGKAIGAWSGLSGVTTAIGPFVG
                     GYLVDAVSWRWIFVINLPLALVAIRIATKHVPESSAGGGHLDLRGAVTAALGLGGVTF
                     ALIEGPVRGWSDPTTVTAMVAGVIAAVVFVRAELTHPDPMLPFDIFRSRQFSGANAAT
                     LLVYFALGGAMFFLVIELQRELGYSALEAGAALIPTTILLLLLSSRAGALASRIGPRL
                     PMTLGPLVAAVGLAWMTWIGPGTAYVTGVLPPVVVFGLGLALTVAPLTTAVLAAVPQE
                     HAGVGSGVNNAVARVAGLLAVALLPAVARGVDFTDGFRRATWISAAICALGGVASWLT
                     IRGGGTNATHVPPCAEQPPEPVRT"
     gene            1683..2267
                     /locus_tag="VEV43_13920"
     CDS             1683..2267
                     /locus_tag="VEV43_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HYP24663.1"
                     /translation="MTEFLDLLDWKRRVFELYAYVRSVPPKRGWHVWREGRDDLFASH
                     AQTPNPDVKPGYFEYDPAARVLGEVVAAPEQELRLPVSTDEVVTATRIGATRFEIGGE
                     QASLDLFWIGGYGGGLFLPFRDETSGQSTYGAGRYLYDTIKGADLGAEDGKLVLDFNF
                     AYNPSCAYDPRWACPLAPPNNRLGVAIEAGEKAP"
     gene            complement(2264..3184)
                     /locus_tag="VEV43_13925"
     CDS             complement(2264..3184)
                     /locus_tag="VEV43_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYP24664.1"
                     /translation="MRRSRSYVLGAALLAFGLVLGACGSGGDDEGAAGGGGGEEKGQV
                     TVGDDSFAESQIVAEMYAQVLEDAGYEVDRKSTKSREVRLPAMESGEIDVAPEYLASL
                     ISVLDPKAGRVSDAGEAVGMLNDDLLTDQGLQVLEPSEAIDTNIFAVTQETAEKYTTL
                     SDLAEDAGDLTLGAPAECPERPYCIPGLKEVYGIEFGKFEPLEYGAATVESLKQGAID
                     VALLFSTDGTVEREGFVVLEDDKGLQAADNITPLVNEEVLDGEVEELLNAVSAALTTD
                     TMTELNARVNVDLEDPADVAAEFLEENGLL"
     gene            complement(3245..3964)
                     /locus_tag="VEV43_13930"
     CDS             complement(3245..3964)
                     /locus_tag="VEV43_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24665.1"
                     /translation="MSGLEQLVAWITDPVNWSGESGVPVRVLEHLRISAISVLAATAV
                     ALPLGLYIGHSRRGETLAVSVANLGRAIPSFAILALAFPLSLRLGLGLSDWPAIAALF
                     LLGIPPILTNSYVGVKGVDADTLEAARGMGMSEREVLRSIEVPLAAPLIVAGLRISAV
                     QVVATATLAALVAGGGLGRFIVDGFKQGDDGMTLAGAILVALLAILTEVALGLVERAT
                     APRHSRRGVFTTRTRLTSRFT"
     gene            complement(3961..4611)
                     /locus_tag="VEV43_13935"
     CDS             complement(3961..4611)
                     /locus_tag="VEV43_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24666.1"
                     /translation="MSELFRWSWVVDNLDEVAARSWEHVVLTLIAVGIGLVISFPIGV
                     YCHRHRRAYGPVATVAGILYTIPSLALFAMLVPFTGLSTTSAEIGLVSYTLLILIRNT
                     VAGLRSVPADAREAAVGMGYSPRQLLWRVELPLALPVIVAGVRIATVTVIGLVTVTAL
                     IGQGGLGHFILLGLERFFSTATILGAVLSVALAVLADRLLALAERVLTPWARRAPA"
BASE COUNT          676 a         1749 c         1635 g          713 t
ORIGIN      
        1 actccgaggg ctcgctgagc gacgtcgagt gcgccggcag cgccagcgtc ccggcggagc
       61 ccacggcttc ctggagcgcc aggaccaccg cctgggcggc gccggcgacc cacccgaggc
      121 tcttcagcga cgagtggacg aacaacgtca tcccgggagc gacccccagc gcgcgcaggt
      181 cccggaccaa ggacgacacg gtcgcagggc gatccgtcgc ggcgacaacc tcctgctccg
      241 acatcgcgct agcttgacac catgagcgcg cccgggaccc tccgctacgg aacgaccgcg
      301 gggcggtggg tcgtcggggc cacggtgctc ggctccggga tcgccttcct cgacgggacc
      361 gtcgtcaacg tcgcgctgcc cgcgatcgac gccgacctcg gggccggtct ctccgggctc
      421 cagtggaccg tcgacgccta cctcctcacg ctctcggcgt tcctgttgct cggcgggtcg
      481 ctcggagacc tcttcggccg ccgccgcatg ttcgtgtacg ggctcggcgg gttcgccgcc
      541 gcgtcgctcc tctgcgcggt cgcgccgggc accgaggcac tcgtcgcggc gcgggccttg
      601 cagggtgtcg ccgccgcgct gctcgttccg gggagcctcg cgatcatctc gacgagcttc
      661 gctcccgagg acgccggcaa ggcgatcgga gcgtggtccg ggctctccgg ggtcacgact
      721 gccatcggcc ccttcgtcgg cgggtacctc gtcgacgccg tctcgtggcg ctggatcttc
      781 gttatcaacc tcccgctcgc gctcgtagcc atccggatcg ctaccaagca cgtgccggag
      841 tcgagcgccg gcggcgggca cctcgacctc cgcggagcgg tgaccgcggc gctcgggctc
      901 ggcggcgtca cgttcgcgct catcgagggc cccgtccgcg gctggagcga tcccacgacg
      961 gtcaccgcca tggtcgccgg ggtgatcgcg gccgtcgtct tcgtgcgcgc ggagctcacg
     1021 catcccgatc ccatgctgcc cttcgacatc ttccgttcgc gccagttctc cggtgcgaac
     1081 gcggcgacgc tgctcgtgta cttcgcgctc gggggggcga tgttcttcct cgtgatcgag
     1141 ctgcagagag agctcggcta ctccgcgctc gaggcggggg ccgcgctgat cccgacgacg
     1201 atcctgctgc tgctgctgtc gtcgcgggcc ggcgcgctgg cgagcaggat cgggccgcgg
     1261 ctcccgatga cgctcggccc cctcgtcgcc gcggtgggtc tcgcgtggat gacgtggatt
     1321 ggcccgggca ccgcctacgt caccggcgtg ctgccgccgg tcgtggtgtt cggtctcggc
     1381 ctcgcgctca cggtcgcgcc gctgacgacg gccgtcctcg cggcggtccc tcaggagcac
     1441 gcgggggtcg gctcgggagt gaacaacgcg gtcgcgcgcg tggcgggtct gctggccgtg
     1501 gcgctccttc ccgcggtcgc gcgaggggtg gacttcaccg acggcttccg gcgcgcgacg
     1561 tggatctccg cggcgatctg cgctctcggc ggtgtcgcgt cctggctgac gatccgcggc
     1621 ggaggaacca acgcgaccca cgtcccgccg tgcgcggagc aaccgccgga gccggtgcgc
     1681 acgtgaccga gttcctcgac ctgctcgact ggaagcggcg cgtcttcgag ctctacgcgt
     1741 acgtccgctc cgtcccaccc aagcgcgggt ggcacgtctg gcgcgaggga cgcgacgatc
     1801 tcttcgcgtc gcacgcacag acgcccaacc ccgacgtgaa gccgggctac ttcgagtacg
     1861 acccagcggc ccgcgtgctc ggcgaggtcg tcgcggcccc cgaacaggag ctccgccttc
     1921 ccgtcagcac cgacgaggtc gtcaccgcga cgaggatcgg cgcgacccgc ttcgagatcg
     1981 gcggcgagca ggcgtcgctc gacctcttct ggatcggcgg ctacggcggc ggcctcttcc
     2041 ttccgttccg cgacgagacg agcgggcaga gcacctacgg cgcggggcgt tacctgtacg
     2101 acacgatcaa gggcgcggac ctgggggccg aggacggcaa gctcgtcctc gacttcaact
     2161 tcgcctacaa cccttcgtgc gcgtacgacc cgcgctgggc ctgcccgctc gccccgccga
     2221 acaacaggct cggcgtcgcg atcgaagcgg gtgagaaggc cccttagagc agaccgttct
     2281 cctcgaggaa ctccgcggcg acgtcggccg ggtcctccag gtcgacgttc acgcgcgcgt
     2341 tcagttcggt catggtgtcg gtcgtcagcg cagcgctcac cgcgttcagc agctcctcga
     2401 cctctccgtc gagcacctct tcgttgacta gcggggtgat gttgtcggcg gcctgcagac
     2461 ctttgtcgtc ttccagcacg acgaagccct cgcgctcgac cgtgccgtcc gtcgagaaca
     2521 acagtgcgac gtcgatcgcg ccctgcttga gcgactcgac ggtcgcggcc ccgtactcga
     2581 gcggctcgaa cttgccgaac tcgatcccgt agacctcctt caggccgggg atgcagtagg
     2641 ggcgctcggg gcactcggcc ggagcaccga gagtcaggtc accggcatcc tcggcgaggt
     2701 cgctcagcgt cgtgtacttc tccgccgtct cttgcgtcac ggcgaagatg ttcgtgtcga
     2761 tcgcctcgga cggctcgagc acctgcagcc cctggtcggt gagcaggtcg tcgttgagca
     2821 tgccgacggc ctcgccggcg tcgctcacgc ggccggcctt cgggtcgagc accgagatca
     2881 agctcgcgag gtactcgggc gcgacgtcga tctcgccgct ctccatcgcc ggcagcctca
     2941 cctcgcgcga cttggtgctc ttgcggtcga cctcgtagcc cgcgtcctcg agcacctgtg
     3001 cgtacatctc ggcgacgatc tggctctcgg cgaagctgtc gtcgccgacg gtgacctgtc
     3061 ccttctcctc gcctccgccg cccccggcgg caccttcgtc gtccccaccc gacccgcaag
     3121 cgccgagcac gagcccgaac gccagcagag ccgccccgag gacgtagctg cgggatctcc
     3181 gcatgggtag ctccttccct ttttcgcgga gcccgcttct ggaccccgcc cgcgaggaga
     3241 aaccctatgt gaaccgcgaa gtcaaacggg tcctcgtcgt gaagacgccg cggcgtgagt
     3301 gacgcggggc ggtcgcgcgc tcgacgagac ccaacgcgac ctcggtcagg atcgcgagca
     3361 aggcgaccag gatcgctccg gcgagcgtca tgccgtcgtc gccttgcttg aagccgtcga
     3421 cgatgaacct tccgagcccg ccgccggcga cgagcgccgc gagcgtcgcg gtcgctacga
     3481 cctggacggc cgagatccgc aatcccgcga cgatcaacgg cgcagcgagg gggacctcga
     3541 tcgagcggag gacctcgcgc tcggacatcc ccatcccgcg cgcggcttcg agcgtgtccg
     3601 cgtcgacgcc cttcacgccg acgtacgagt tcgtgaggat cggcgggatc ccgagcagga
     3661 acagagccgc gatcgccggc cagtcgctga ggcccagccc gagccgcagc gagagcggga
     3721 acgcgagcgc caggatcgcg aacgaaggga tcgcgcggcc caggttcgcg accgacacgg
     3781 cgagcgtctc gccccgccgt gagtgcccga tgtagagccc gagcgggagc gccaccgcgg
     3841 tcgccgccag gaccgagatc gccgagatcc gcaggtgctc gagcacgcgg acgggaaccc
     3901 cgctctcacc cgaccaattg accgggtcgg tgatccatgc gacgagctgc tctaagccgc
     3961 tcatgccggc gccctccgcg cccacggcgt aagcacacgc tcggcgagcg cgagcagccg
     4021 gtcggcgagc accgccagcg cgaccgacag gaccgcgccg agaatcgtcg ccgtcgagaa
     4081 gaaccgttcg agcccgagca ggatgaagtg gccgaggccc ccctgcccga tgagcgcggt
     4141 gaccgtgacg agcccgatca ccgtcaccgt cgcgatcctg acgccggcga ctatgaccgg
     4201 gagcgcgagc gggagctcga cgcgccacag gagctgccgc gggctgtagc ccatcccgac
     4261 ggcggcctcg cgggcgtcgg ccggcacgga gcgcagcccc gcgaccgtgt tgcggatcag
     4321 gatcagcagc gtgtagctga cgaggccgat ctcggccgac gtagtcgaga gcccggtgaa
     4381 cggcacgagc atcgcgaaca gggcaaggct cgggatcgtg tagaggatgc cggcgacggt
     4441 cgcgacgggg ccgtaggcgc gccggtggcg gtggcagtac acgccgatcg ggaacgagat
     4501 gacgagccct atcccgacgg cgatcagcgt gaggacgacg tgctcccacg agcgcgcggc
     4561 gacctcgtcg aggttgtcga cgacccagct ccagcggaac agctcgctca agcttcgagc
     4621 tccgctcgga tcgcttcgat ggtcagcacc ccgtcctcgc cctcgatccc gaccacgccg
     4681 ctcttcgaag cgacgaccgc gtcgagcgcc tcgagcagcg acgtgtcgcg gcccacgcgc
     4741 acttcgaccg gtccgtccgg cggcgccagc cgg
//
LOCUS       DASQDO010000509         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_481529, whole genome shotgun sequence.
ACCESSION   DASQDO010000509 DASQDO010000000
VERSION     DASQDO010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481529"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VEV43_13940"
     CDS             <1..199
                     /locus_tag="VEV43_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24667.1"
                     /translation="GSQTTTLAEMVDRLRAGLYSFTWGLDEAARNRGADAAAALARER
                     YGSLEEPREIPQEMYWRAYDL"
     gene            complement(257..580)
                     /locus_tag="VEV43_13945"
     CDS             complement(257..580)
                     /locus_tag="VEV43_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24668.1"
                     /translation="MKAVVWRANEQNEAVARGYSYVPNPGCYEYFQITVLLKKNGTGV
                     NVNYCDLSNSLRNSEADPNQCSTRVSDPLGPQDWTAHVKVTWRKAGTCCNNVTTYINT
                     PVDIF"
     gene            complement(838..1914)
                     /locus_tag="VEV43_13950"
     CDS             complement(838..1914)
                     /locus_tag="VEV43_13950"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HYP24669.1"
                     /translation="MAERIVETVGILGAGQLGRMLAVAASRLGLRTHVYAPDPDPIAA
                     QVANAMTSAPYEDATALSEFADAVDVVTYEFENVPAEALDQLESARKLLPRRNALTIS
                     QDRLLEKDFLNDAGLTTAPYANVETESDAAEAATKVGSPSIMKTRRLGYDGKGQVRLD
                     RPEDAAPAWTELGNVPCIVEGFVDYRCEISVIAARSLRGEVVCFEPGENVHRDGILRT
                     TTIPAAISEAERAEALSAASTILQRLDYVGVMGIELFVTADGLAVNEIAPRVHNSGHW
                     TQNGCAVDQFEQHIRCVAGWPLGDGGRHSNVEMTNLIGDDVLNLPELARDPALALHVY
                     GKADVRPGRKMGHVNRITGPAGSL"
     gene            complement(1907..2392)
                     /gene="purE"
                     /locus_tag="VEV43_13955"
     CDS             complement(1907..2392)
                     /gene="purE"
                     /locus_tag="VEV43_13955"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYP24670.1"
                     /translation="MSALVGIVMGSQSDWATMKHVAEVLDELGVTYETKIVSAHRTPD
                     RLWAYGKSAVERGLRVVVAGAGGAAHLPGMMASKTRLPVIGVPVQSKALQGLDSLLSI
                     VQMPAGCPVATMAIGPAGAKNAGLMAAAILALSDQALGERLDRWRERQSDAVPEEPSD
                     G"
     gene            complement(2389..>2914)
                     /locus_tag="VEV43_13960"
     CDS             complement(2389..>2914)
                     /locus_tag="VEV43_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HYP24671.1"
                     /translation="VQPMVDETARRGAPFHGALYAGLALTAGGPKVIEFNARFGDPET
                     QALIPRLQSDFGEVVLATATGDLSGTKLEWSSRPCVTIVLASGGYPGPYDTGYEIHGL
                     DDAAAVSDVQVFHAGTRAEGDSVVTAGGRVLNVSALGDTFAAARERAYEAASRIEFEK
                     VHFRRDVAERAVKQ"
BASE COUNT          427 a         1048 c          949 g          490 t
ORIGIN      
        1 ggggtcgcag acgacgacct tggccgagat ggtcgaccgg ctacgggcgg gcctctactc
       61 gttcacgtgg gggctggacg aggccgcacg gaaccggggc gcggacgcgg cggccgcgct
      121 ggctcgagag aggtacgggt cactggagga gccgcgcgag atcccgcagg agatgtactg
      181 gagggcctac gacctgtaga tgccgcgcgc aaaggaggga gccatcggcc ccctccttcg
      241 tcgcagcgac caagcgctag aagatgtcca ccggcgtgtt gatgtacgtc gtgacgttgt
      301 tgcagcacgt tccggctttg cgccacgtca cctttacgtg cgccgtccag tcttgcggcc
      361 cgagcgggtc cgacactcgg gtcgagcact ggttcggatc ggcctctgag tttctcagcg
      421 agttcgagag gtcgcagtag ttgacgttca cgcctgtgcc gttcttcttc aacaagaccg
      481 tgatctggaa gtactcgtag cagcccgggt tggggacgta cgagtaaccc ctcgccacgg
      541 cttcgttttg ctcgttcgcc ctccagacga cggccttcat gtttagcgct tttccgtcac
      601 atccgggctt cgtcacccat cccgtccccg ccgtggcgcc tgccggcggc atcaccgcgg
      661 ccaagcaccc cattacgagc atcagtacga cagcaatacg tccgcgcatc tcgtgccccc
      721 ctcgtcacct gacctgtcgg ccaccacgtt acggagggag ataaggccgc gctatagctc
      781 aataggacta ttcggtacaa atgtcgtaac cggatcgact gcgacgaacg gacgcgccta
      841 cagcgagccg gcagggcctg tgatcctgtt cacgtggccc atcttgcggc ccggcctcac
      901 gtcggccttg ccgtacacgt gcagcgccag cgccgggtcg cgtgccagct cgggcaggtt
      961 caggacgtcg tctccgatca ggttcgtcat ctctacgttc gagtgacggc ccccgtcgcc
     1021 cagcggccat cccgcgacgc aacggatgtg ctgctcgaac tggtcgaccg cgcacccgtt
     1081 ctgcgtccag tgccccgagt tgtgcacgcg cggcgcgatc tcgttcaccg ccaggccgtc
     1141 cgcggtcacg aacagctcga tccccatcac gccgacgtag tcgagccgct ggaggatcgt
     1201 gctcgcggcg ctcaacgcct ctgcacgttc ggcctccgag atcgccgcgg gaatcgtcgt
     1261 cgtgcgcagg atcccgtcgc ggtggacgtt ctcgccgggc tcgaagcaaa cgacctctcc
     1321 gcgcagcgac cgcgccgcga tgaccgagat ctcgcaccgg tagtcgacga acccctcgac
     1381 gatgcacggg acgttcccca gctcggtcca cgcgggggcc gcgtcctccg gccggtcgag
     1441 gcggacctgt cccttcccgt cgtagcccag ccgacgcgtc ttcatgatcg acggcgaccc
     1501 gaccttcgtc gcggcctcgg ccgcgtcgga ctcggtctcg acgttcgcgt acggcgccgt
     1561 cgtcagacct gcgtcgttga ggaagtcctt ctcgaggagg cgatcctggc tgatcgtcag
     1621 agcgttgcgc cgcggcagga gcttgcgtgc ggactcgagc tggtcgaggg cctcggcagg
     1681 aacgttctcg aactcgtacg tgacgacgtc gaccgcgtcc gcgaactcgc tcaacgcggt
     1741 cgcgtcctcg taaggagcgc tcgtcatcgc gttcgccact tgcgccgcga tcgggtccgg
     1801 gtcgggggca tacacgtgcg tcctgaggcc gagccgcgac gccgccaccg cgagcatgcg
     1861 gccgagctgg ccggccccca ggatcccgac ggtctcgacg attcgctcag ccatccgacg
     1921 gttcttcggg aacggcgtcc gactgccgtt cccgccaccg gtcgagcctc tcgccgagag
     1981 cctggtcgct cagcgccagg atggccgcgg ccatgagccc ggcgttcttc gcgcctgcgg
     2041 ggccgatcgc catcgtggcg acggggcacc cggccggcat ctgcacgatc gagagcaggc
     2101 tgtcgagacc ctgcagcgcc ttgctctgga ccggaacccc gatcaccggc agccgcgtct
     2161 tcgacgccat catccccggg aggtgcgcgg ccccgcccgc gcccgctacg acgacccgca
     2221 accctcgctc gaccgcgctc ttgccgtagg cccagaggcg gtccggcgtg cgatgcgccg
     2281 agacgatctt cgtctcgtac gtcacgccca gctcgtccag cacttcggcg acgtgcttca
     2341 tcgtcgccca gtcggactgg ctgcccatga cgatgccgac gagcgcgctc actgcttgac
     2401 ggccctctcg gcgacgtccc tgcggaagtg gaccttctcg aactcgatgc gcgacgcggc
     2461 ctcataggcg cgctcgcgcg ccgctgcgaa cgtgtcgccc agcgcgctca cgttcagcac
     2521 gcggcccccg gcggtgacga cggaatcgcc ttccgcgcgc gtccctgcgt ggaacacctg
     2581 cacgtccgag accgccgccg cgtcgtcgag gccgtggatc tcgtagccgg tgtcgtaggg
     2641 gcccgggtac ccgccgctcg caagcacgat cgtcacgcac ggtcgcgagg accactcgag
     2701 cttcgttccg gagaggtcgc ccgtcgcagt cgcgagcacg acctcgccga agtcggactg
     2761 gagccgcggg atcagcgcct gcgtctcggg gtcgccgaag cgcgcgttga actcgatgac
     2821 cttcggcccg ccggcggtga gcgcgagccc cgcgtagagc gcgccgtgga acggcgcacc
     2881 gcgccgcgcg gtctcgtcga ccatcggctg gacg
//
LOCUS       DASQDO010000510         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_542266, whole genome shotgun sequence.
ACCESSION   DASQDO010000510 DASQDO010000000
VERSION     DASQDO010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542266"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VEV43_13965"
     CDS             <1..764
                     /locus_tag="VEV43_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HYP24672.1"
                     /translation="VAVSPDGRVTSGSPGLYEDPHIEAWKIVLPQGAAARLVHAGRRG
                     ATKPRHLGLDQPSDDGWPLIAPAAVAYGSNAVPREATAEDMERVVEDFAAAARRAAPA
                     GFAALIVDCARGYLLGSFLSPATNRGSEEIEERAGFPLEVFDAVRREWRGPLGVSLNA
                     FDGVRGGIDFDDVLWVARALREKGCDFVEVTAGGTTPASVVPYDPYTLPSYADRIRNE
                     AGVPVLLGGAVSTVDRVNTLVASGRADLCAVMPPR"
     gene            complement(714..1640)
                     /locus_tag="VEV43_13970"
     CDS             complement(714..1640)
                     /locus_tag="VEV43_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP24673.1"
                     /translation="MRAADSMLVAKATWLATRAAAAVSPRLAGPIAARLWFTPWRVPV
                     GERAAKRQAEWLRPTEALSLEVDGKRIAGFAAGKGPTVLLVHGWGERGASLGAFVRPL
                     VDAGYRVVGVDLPGHGGSEGGQSDGFQIASAIAGVAEALGGVRAVVAHSMGGTTSLYA
                     ASEGMPVDALVLIAPSPRLDHALKTFARLLSLSPNAVAGLKATIERRYGRGVWGTFSG
                     LRVASNVKVPGLIVHDREDPQVALADAEALHAAWPGSRLVTTESLGHGRILRDEKVIE
                     AVTSFLAEVAPARPVPLPRGHDRAEVRTPARH"
     gene            1862..2350
                     /locus_tag="VEV43_13975"
     CDS             1862..2350
                     /locus_tag="VEV43_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP24674.1"
                     /translation="MPPPTDFSAMDCSVARTLDVIGDKWSLLILRDAFYGVRRFDDFR
                     EDLGIARNVLTDRLQKLVATGVFERRRYEERPPRHEYRLTEKGRALLPVLLAMIAWGD
                     RWANDSDIPPVTLTHTTCGCVTHASVVCDNCGDELTLREVRVDPIRVKGANRAAALEG
                     AV"
     gene            complement(2354..2755)
                     /gene="mscL"
                     /locus_tag="VEV43_13980"
     CDS             complement(2354..2755)
                     /gene="mscL"
                     /locus_tag="VEV43_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HYP24675.1"
                     /translation="MLKEFKGFVLRGNVVDLAVGVVIGAAFGTVVTSFVENVLTPIVS
                     IPGSVDFADWKREVSGSTIYFGEFLNDLISFVLIAAAVFFFVVRPVNTLMARRKTERD
                     VTSESKQCPECMSNIPKAAKRCAFCTSVQAA"
     gene            2821..3678
                     /locus_tag="VEV43_13985"
     CDS             2821..3678
                     /locus_tag="VEV43_13985"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HYP24676.1"
                     /translation="MSLVEVVNEPEGLTAPALVVSFEDWVDAGAAGTTAARHVATGGE
                     VVARFDVDALYDYRSHRPVLDIVDGVPSRLQWPALELTRRRLPERDVFVLTGPEPDYR
                     WQELAADVYELGVRFGVIEHVSLGAIPAAVPHTAPTPVMMTASGEELLRGARPNEGLL
                     RVPAAAVSVIEWTMAQNGIPAVGFWAQVPHYATPYAAGAIALVRRVEDHLSVTVGAGE
                     LEEQVVAQRTALEQIFATNPEAREHLGRLEGLVGETEVPPAENIAAEVEKFLRNQRRG
                     EGGPTPYSP"
BASE COUNT          571 a         1367 c         1340 g          525 t
ORIGIN      
        1 acgtcgccgt gtctccggac gggcgggtca cgtccgggag ccccggcctc tacgaagacc
       61 cacacatcga ggcgtggaag atcgtgctcc cgcagggggc cgctgcccgg ctcgtgcacg
      121 caggacgccg cggcgccacc aagccccggc acctcggtct cgaccagccc tcggacgacg
      181 gatggccgtt gatcgccccc gcggcggtcg cctacggatc gaatgccgtc ccacgcgagg
      241 ctacggccga ggacatggag cgcgtcgtcg aggacttcgc cgccgcggct cggcgcgcgg
      301 cccccgcggg cttcgccgct ttgatcgtcg actgcgcgcg cggctatctc ctcgggagct
      361 ttctctcgcc ggccacgaac cgaggcagcg aagagatcga agaacgggcc gggttcccgc
      421 tggaggtctt cgacgccgtg cggcgggagt ggcgcggccc cctcggcgtc tcgctgaacg
      481 ctttcgacgg agtgcgcggt gggatcgact tcgacgacgt cttgtgggtc gcccgcgcgc
      541 tgagggaaaa ggggtgcgac ttcgtcgagg tcacggcagg aggcactact ccggcgagcg
      601 tcgtgcccta cgacccctac acgctcccga gctatgccga ccgcatccgc aacgaggcgg
      661 gtgtgccggt gctgctcggc ggcgcggtct cgaccgtcga ccgcgtgaac acgctagtgg
      721 cgagcgggcg tgcggacctc tgcgcggtca tgcccccgcg gtagcgggac cggccgcgcc
      781 ggggccacct cggcgaggaa cgacgtgacg gcctcgatca ccttctcgtc gcgcaggatc
      841 cggccgtgcc ccagcgactc cgtcgtcacg agccgggagc cgggccacgc cgcgtggagt
      901 gcctctgcgt cggcgagcgc gacctgcggg tcctcccggt cgtgcacgat caggccgggg
      961 accttgacgt tggaagcgac cctcaagccc gagaacgtgc cccagacacc gcggccgtag
     1021 cggcgctcga tcgtcgcctt caaccccgcg accgcgttcg gcgacagcga caggaggcgc
     1081 gcgaacgtct tcagcgcgtg gtcgaggcgc ggcgacggcg cgatcagcac cagagcgtcg
     1141 acgggcatgc cctcgctcgc cgcgtacaga gaggtagtgc cgcccatcga gtgcgcgacg
     1201 acggcgcgga cgccgccgag cgcttccgcc accccggcga tcgcagacgc gatctggaag
     1261 ccgtcgctct gcccgccttc gctcccgccg tgcccgggca ggtcgacacc gacgacgcgg
     1321 tagcccgcgt ccaccagcgg ccggacgaac gctccgagac tggctccgcg ctcgccccag
     1381 ccgtgcacga gcaggacggt ggggcccttc ccggccgcga agccggcgat ccgcttgccg
     1441 tcgacctcga gcgacagggc ttccgtcggg cgcagccact cggcctgccg ctttgccgcc
     1501 cgctcgccga ccgggacgcg ccacggcgtg aaccacaagc gtgccgcgat gggcccggcg
     1561 agacgggggg agacggcggc cgcggcccgc gtcgcgagcc acgtcgcctt cgccacgagc
     1621 atgctgtcgg cggctcgcat ctagccgatc tccgcagggg tcgagtccgg gatctcccgc
     1681 caggccgacc atcccgggtc gaggtcgtcc atgatctccg tcagctccac gcgtaccggc
     1741 tccatccacc aggtcgtcat cccggcctcc tttcgggcta gtgagttgtc tttcgcaacc
     1801 cactatcgac gaccctagcg gaggaggttg caaaaagcaa ctgaccccgt atccttaggg
     1861 catgccccca cccacggact tctccgccat ggactgctcg gtcgcccgga cgctcgacgt
     1921 catcggcgac aagtggtcgc tgctgatcct ccgagacgcc ttctacggcg tccgccgctt
     1981 cgacgacttc cgcgaggacc tcgggatcgc gcgcaacgtg cttaccgaca ggctccagaa
     2041 gctcgtcgcg accggcgtgt tcgaacgccg ccgctacgag gagcggcccc cgcgccacga
     2101 gtaccggctg accgagaagg gccgtgcgct gctgccggtc ctgctcgcga tgatcgcctg
     2161 gggcgaccgc tgggcgaacg actcggacat cccgccggtg acgctgacgc acacgacctg
     2221 cgggtgtgtg acgcacgcgt cggtcgtctg cgacaactgc ggcgacgagc tgacgctgcg
     2281 cgaggtccgc gtcgacccga taagggtcaa gggcgcgaac cgggccgcgg ccctcgaagg
     2341 cgccgtctag gccctacgcc gcctgcacgc tcgtgcagaa cgcgcagcgc ttcgccgcct
     2401 tcgggatgtt gctcatgcac tccgggcact gcttcgactc gctcgtcacg tcgcgctcgg
     2461 tcttgcgccg cgccatcagc gtgttgacgg gcctgaccac gaagaagaag accgccgccg
     2521 cgatcagcac gaacgagatc aggtcgttga ggaactcgcc gaagtagatc gtgctgccgc
     2581 tgacctccct cttccagtcg gcgaagtcca ccgatcccgg gatcgagacg atcggcgtca
     2641 gcacgttctc gacgaacgaa gtcactacgg tcccgaacgc cgcgccgatg acgacgccga
     2701 cggcgaggtc gacgacgttg ccccgcagga cgaacccctt gaactccttc agcatcgcga
     2761 gccccctcgg tagacggtgg gcagatcgta gcgagcggcc cgacgtgatt agctgtcgcg
     2821 atgagcctcg tcgaggtcgt gaacgagccg gagggcctca ccgcgcccgc gctcgtcgtc
     2881 tcgttcgagg actgggtcga cgcgggggcc gccggcacga ccgccgcccg gcacgtggcg
     2941 accgggggcg aggtcgtcgc gcggttcgac gtcgacgccc tctacgacta ccgcagccat
     3001 cgaccggtgc tcgacatcgt cgacggcgta ccgagccgcc tccagtggcc ggcgctcgag
     3061 ctgacgcgca gacggctgcc cgagcgtgac gtcttcgtgc tgacgggacc ggagccggac
     3121 taccggtggc aggagctggc cgccgacgtc tacgagctcg gggtgcggtt cggcgtgatc
     3181 gagcacgtca gcctcggcgc gatccccgcg gcagttcccc acaccgcgcc gacccccgtg
     3241 atgatgacgg cgtcgggcga ggagctcctg cggggagcgc ggccgaacga ggggctgctg
     3301 cgcgtgccgg cggccgcggt cagcgtgatc gagtggacga tggcgcagaa cggcataccg
     3361 gcggtcggct tctgggcaca ggtcccgcac tacgcgacgc cgtacgcggc cggcgcgatc
     3421 gcgctcgtgc gccgggtcga ggaccacctg tcggtgaccg tcggtgccgg cgagctcgag
     3481 gagcaggtgg tcgcgcagcg gacggcgctc gagcagatct tcgccacgaa ccccgaggcc
     3541 cgcgagcacc tcggacggct cgaggggctc gtcggcgaga ccgaggtgcc gccggcagag
     3601 aacatcgcgg cggaggtcga gaagttcctg cgcaaccagc gacggggcga ggggggcccg
     3661 acgccgtaca gtccctagcc ggacgagcga gaggggcggg cacgtggacc accaggtggg
     3721 cgacgacacc ctcgtcggga aggtgtcgcg caggcatcct gcgagtggcg atccgtacaa
     3781 gagcgcgcgg cgggtagcga cgg
//
LOCUS       DASQDO010000511        11232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1094103, whole genome shotgun sequence.
ACCESSION   DASQDO010000511 DASQDO010000000
VERSION     DASQDO010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094103"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1497
                     /gene="zwf"
                     /locus_tag="VEV43_13990"
     CDS             31..1497
                     /gene="zwf"
                     /locus_tag="VEV43_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYP24677.1"
                     /translation="MTPPRREPDACVVVIFGASGDLTARKLLPALFNLRREGLAPGAT
                     SVLGTARTEMSDAEFATTMRRAVTDHSRVEPTNETWGGFADNLFYVPGDLSSDKTYAA
                     LKEKLAEIDEVHGTGGNRVWYLATAPQFFPAIADGIGKSGLLDTGGWHRLVVEKPFGR
                     DLDGARELNGVLDRTFSEDQIFRIDHYLGKETVQNLLVFRFANAVFEPIWNRRYVDNV
                     QVTVAEDGGIGSRGAFYDHTGALRDVAQNHLLQLLTLVAMEPPVSWDADSIRNEKVQV
                     LKAVRRWPPSECGTLAARGQYEGYRDVDGVDPQSSTETFVALKLFVDNWRWAGVPFYL
                     RTGKAMPATATEITIQFQSVPHLLFAKTAVEELEPNVLVLRIQPNEGISLTFGAKVPG
                     HEVNVRTVDMEFDYESDFGSGSPEAYERLLQDCMLGDATLFTRRDEIEQAWEIVEPVL
                     EYWSGGGRPGGYARGAWGPPSSDELLRRDGRRWRDPVV"
     gene            1494..2147
                     /gene="pgl"
                     /locus_tag="VEV43_13995"
     CDS             1494..2147
                     /gene="pgl"
                     /locus_tag="VEV43_13995"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HYP24678.1"
                     /translation="MSASVRVLGDFVAEAAAVLGGLLPATGSVVITGGGTVSEVYPLL
                     AGARPDWSGLDVFFSDERCVPPDDAASNYGMAKRTLLDAVGPRAVHRMRGEIDPQEAA
                     ALYDEAVVDPFDVVILGLGDDAHVAGLFPGSPGLLEAASCVVVDRPDGMQGLSLTPPA
                     LLSGRGVVFLVSGPSKAEAVRRAVAADEDVMACPAAMFRDHGDVTFLLDAAAAAELS"
     gene            complement(2223..3869)
                     /locus_tag="VEV43_14000"
     CDS             complement(2223..3869)
                     /locus_tag="VEV43_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24679.1"
                     /translation="MAKARPEGRLRSGFSLGEEQAELDSLLEAAFYESGHYSIISSHG
                     DNRCFVVARTGGGKSAALRHLQQVNGDGVVKIDPGDISLLYFSTSNVLKYLSDLQVHL
                     DPFFIALWKHVLVVELIRRRYKIDSDEAKSGIWAGLVERLRRDKSKQLALDYFNDFEG
                     RFWDDTDVRLRAITTRLEKKIDATRSGDVGASGVAHIAAGETASAGEMIEERIELAKK
                     FQAIVNETQLSRLTKMIDILDEDILDSEENRMFIVIDDLDRDWIDHRVVNDLIRCLLL
                     TVLDLQRVRNLKVLVALRTNIFSQLDFGRAGAQEEKLRALVLQMKWSKHELVALLDER
                     VKVAAEKVGREHIGGIKDLLPPKSRRRGAALDFILGRTLMRPRDAMAYLNECLAQSAG
                     KPRLTWNVILGAEDPYSYKRLLALRDEWKLTYPGIDRVFALFQGELMPMRRDAYLKHL
                     GDALLLHLDETFDGSTWMTSLSTRMWDPPETDWRQIYHPLHELLFRVGFIGFSRTPGQ
                     GVVYAHEDMDYGSSIGNLRDASLFCVHPTFHAALEIKTPS"
     gene            4143..5516
                     /locus_tag="VEV43_14005"
     CDS             4143..5516
                     /locus_tag="VEV43_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HYP24680.1"
                     /translation="MRSDKGQRRLTLEDERIRMDTGDTAFILVCSALVMFMTPGLALF
                     YGGMVRSKNVLGTIMQSVFALGVVSVLWVVVGYTLAFGPDNGGLIGGLDFLGFSGVGA
                     EPNADLAATIPHSLFAVFQMMFAIITPALITGAFAERMKFSGYVAFTALWLLVVYAPV
                     AHWVWAPGGWIREMGALDFAGGTVVHINAGIAALAAAVVVGKRRGFGKEAFVPHNLTM
                     TVLGTGILWFGWFGFNAGSALGANGLAASAFLATNLGAAAGACGWALVDARKEKKSTT
                     LGVASGAVAGLVAITPAAGFVGPLAAIAIGLAGGVVCAYAVRLKFRFGYDDSLDVVGV
                     HLVGGIVGAFLTGVFADTAINSAGVDGLLAGGGAALLGKQAVAIGATLLFSFVVSVGL
                     LKLVEATIGLRVTEEEEATGLDLAQHAESGYAFAEGGGGFSPAPAAAPVPAHAPAAVR
                     VPRGSEA"
     gene            5517..5855
                     /locus_tag="VEV43_14010"
     CDS             5517..5855
                     /locus_tag="VEV43_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYP24681.1"
                     /translation="MKVVVAVVKPFKLEDVKEALEGIGVQGLTVSDARGFGRQKGHTE
                     VYRGAEYQVDFLPKTRIEVAVDDDQVDDVVKAIVTSARTDSIGDGKIWVVPAEQVVRI
                     RTGEKGPDAL"
     gene            5885..8242
                     /gene="glnD"
                     /locus_tag="VEV43_14015"
     CDS             5885..8242
                     /gene="glnD"
                     /locus_tag="VEV43_14015"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HYP24682.1"
                     /translation="MSDGTTVAAARSGDVDAALRELADAALGPAGGVTLVAVGGYGRR
                     EMSPHSDVDLLCLVPRKPDVSPATLRGLLYPLWDAGFQVGHAVRTPAEAVEHATKDLD
                     AATSLLSARLVAGDEAAFAELVDRRARWLRRGARALVRTIVSATEARHSSRERAGWSL
                     APDLKEDVGGLRDLHAMGWLAAAGGENTTGLDEERSVLLAAREALHAQSKRKLDRLRI
                     DLQPAVARELGFDDPDELMERVHSSARRIESRTRRTLNDLAARGPRRSGSALEVAPGV
                     RLDDGALGLTVPPDPVTATSLLAAHATLAKPLSARSLDSLDSAFAGEPQERWDPRLRD
                     AFVRLLGGPAASSALELLDHAGAWPVLLPEWSGARGRAQHDPYHRYTVDGHSFVAVDA
                     ANRVLAEDGLARDAGSTDALLVATLLHDAGKGSGEDHSVAGERLARSAATRMGFDHAT
                     VEEIATLVRWHLLLVDTATRRDLDDGAVIEDVAARVGTPRLLRLLYVLTVADATATGP
                     EAWTPWKASLVRDLFRKALVALETGALPARSDVSTKAREVIAFEPALAGSAEDVLTTL
                     PPSYLGSATVAEIADDIRLLLRRPRPGDVVARVEDGTEDGTEVLTVCVADRPGSLART
                     AGVLAMNLVTVLSAQAFSTTEGLALQRLTVRPDDPARMESVVAGLEAAFAGRIALEAR
                     LTKKALDYRPAGEVAVDVRVVDDASVHSTVVEVRGPDALGLLYAIAAGLSDLDLDIHV
                     AKIDTLGARVVDTFYVRDARGTKPGPEQTKEIERAVAHRVSSLYG"
     gene            complement(8239..9552)
                     /locus_tag="VEV43_14020"
     CDS             complement(8239..9552)
                     /locus_tag="VEV43_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYP24683.1"
                     /translation="MEDPLSLGIGALRRALKRGELDAREVAAAALDRAGSQELNAFVE
                     TRAEAVDEAGRASGPLAGVPLAVKDMFVDEGRVPTCGSNAHGTWLTGTATIVERLRRA
                     GAVVVGYTNLHEWGVGMTSAVTATGPIRNPWDPALVPGGSSGGSAAALAAGIVPAAIG
                     TDAGGSIRCPSACCGTPGLKAPGGRLPTKGFVGDGGAIDGVGPMARSVEDVALLFGVM
                     ADEELSLPEGESLRLGIPSSFFFDDLDPGVGAAVESGLEVLRGLAADVREVDLPAAAT
                     ASFAVPALLLPLFAERLSGVDDGAFQPPTAHLIALGRSATEDDRRQAAGTQEAMAADW
                     ARVFEEVDVVVTPTLPGPPAPVDTLLVELPSGPAAAELAYLRTNAPMNLGGVPALSLP
                     CGVMPSGLTTNLTLTAARGRDELALAMGLAFERATDGAYANRIAR"
     regulatory      9654..9728
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            9743..10252
                     /locus_tag="VEV43_14025"
     CDS             9743..10252
                     /locus_tag="VEV43_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYP24684.1"
                     /translation="MNAFNRAVIGFTATALFVGAAPAITAGAQTKQGGCWDVKGAERG
                     FAKRINDVRGVARAGTLRLDPELSRAARLHTREMVKQQKLYHTPSDKLSKRVTNWTVL
                     GENVGVGGDVQSLHDAFMASPGHAKNVLYDSFRHVGIGTIKSRGQLWVTVIFEAETNP
                     GTTLNMPSC"
     gene            complement(10541..>11232)
                     /locus_tag="VEV43_14030"
     CDS             complement(10541..>11232)
                     /locus_tag="VEV43_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24685.1"
                     /translation="AALPAIRKDPAAAMGAGKTTSVMNAGAEPRRVTYGESSAVAGTL
                     TLEDGSPLAFQSVVLYTNERGRGWTPAGTTTTGADGSFAFSVAPQADTKAFVVYDGND
                     TTWGSQSRQIRIRVQPEVTLVPEGAIADAFGTYHYPAGTTSARLTGNVAPPHAGKRVS
                     VRVWQVSVEGTMTLLKKRIRRLDESGSYTTEVALPNTAPGNRYRAITWFEGDADHTKA
                     PSPEVFFTVGA"
BASE COUNT         1736 a         3845 c         3862 g         1789 t
ORIGIN      
        1 agccgacttc ctcgacgagg tctgggccgg gtgacgcctc cgcggagaga gcccgacgcc
       61 tgcgtcgtgg tgatcttcgg cgcgtcgggc gacctcaccg cgcgcaagct cctgccggcg
      121 ctgttcaacc tgcggcgcga gggcctggcg cccggggcga cctccgttct cgggacggca
      181 cgtacggaga tgtcggacgc ggagttcgct acgacgatgc gccgcgcggt gaccgaccac
      241 tcgcgcgtgg agccgacgaa cgagacctgg ggcgggttcg ccgacaacct cttctacgtc
      301 cccggcgacc tctcgtccga caagacctac gccgcgttga aggagaagct cgccgagatc
      361 gacgaggtgc acggcacggg gggcaaccgc gtctggtacc tcgcgaccgc gccgcagttc
      421 ttccccgcga tcgccgacgg gatcggcaag agcgggctgc tcgacaccgg cgggtggcac
      481 cgcctcgtgg tcgagaagcc gttcggccgc gacctcgacg gagcgagaga gctcaacggc
      541 gtcctggatc gcaccttctc cgaggaccag atcttccgca tcgaccacta cctcggcaag
      601 gagacggtgc agaacctcct cgtcttccgg ttcgccaacg cggtgttcga accgatatgg
      661 aaccggcgct acgtcgacaa cgtgcaggtg accgtggccg aggacggtgg catcggttcg
      721 cgcggagctt tctacgacca cacgggcgcg ctgcgcgacg tcgcgcagaa ccatctcctc
      781 cagctcctga cgctcgtcgc gatggagccc ccggtctcgt gggacgcgga ctcgatccgc
      841 aacgagaagg tccaggttct gaaggccgtg cggcgctggc cgccgtcgga atgcgggacg
      901 ctcgcggcac gggggcagta cgagggctac cgggatgtgg acggcgtcga cccgcagtcg
      961 tcgacggaga cgttcgtcgc gctcaagctg ttcgtcgaca actggcgctg ggccggcgtc
     1021 ccgttctacc tccggaccgg caaggccatg ccggcgacgg cgaccgagat cacgatccag
     1081 ttccagagcg tcccgcacct gctgttcgcc aagacggccg tcgaggagct cgagccgaac
     1141 gtgctggtgc tgaggatcca gccgaacgag ggcatctcgc tgacgttcgg cgcgaaggtc
     1201 cccggccacg aggtcaacgt gcggacggtc gacatggagt tcgactacga gagcgacttc
     1261 gggtccggca gcccggaggc ttacgaacgc ctgctccaag actgcatgct cggcgacgcg
     1321 accctgttca cgcggcggga cgagatagag caggcctggg agatcgtcga gccggtgctc
     1381 gagtactgga gcggcggggg ccggccgggc ggctacgccc gcggggcgtg ggggccgccg
     1441 tcgtcggacg agctgttgcg ccgcgacggc cggcgctggc gcgacccggt cgtatgagcg
     1501 cttcggtaag ggtcctcggc gacttcgtag cggaggctgc ggcggttctc ggcgggctcc
     1561 ttcccgccac ggggtccgtc gtgatcaccg ggggcgggac cgtttcggag gtctatccgc
     1621 tgctcgcggg ggcccggccc gattggtcgg ggctcgacgt cttcttctcg gacgagcggt
     1681 gtgtgccgcc ggacgacgcc gcgagcaact acgggatggc gaagaggacg ttgctggacg
     1741 cggtggggcc gcgagcggtc caccggatga ggggcgagat cgatccgcag gaagcggccg
     1801 cgctctacga cgaagccgtc gtcgatccgt tcgacgtcgt gatcctgggc ctcggcgacg
     1861 acgcgcatgt ggcggggctg ttcccgggct cgccggggtt gctggaggcg gcgtcgtgcg
     1921 tggtggtcga ccggccggac gggatgcagg gtctgtcgct gacgccgccg gccctgctgt
     1981 cgggccgcgg ggtggtcttc ctcgtctcgg gtccgtcgaa ggcggaggcc gtgcgccgag
     2041 ccgtggcggc ggacgaagac gtgatggcct gcccggccgc gatgttccgc gaccacggcg
     2101 acgtcacgtt cttgctggac gccgccgcgg ccgcggagct ctcttaatca ctgccatggc
     2161 ggcgcgccgg ctcctatccg ccccgtcggt gatttgttcc gaccaggcgc aagagttcga
     2221 ggtcacgacg gggtcttgat ctcgagtgca gcgtgaaacg tgggatggac gcagaatagc
     2281 gacgcatcgc gaaggtttcc gattgagctc ccatagtcca tatcttcatg cgcataaacg
     2341 acgccttgtc ccggcgtcct actgaacccg atgaacccaa ctcgaaacag cagttcgtgc
     2401 aaggggtgat agatctgtct ccaatccgtt tcgggagggt cccacatcct ggtggagagc
     2461 gacgtcatcc atgtactgcc gtcgaacgtc tcgtctaagt gaaggaggag ggcgtcgccc
     2521 agatgcttga ggtacgcgtc tcgtctcatc ggcatcagct cgccctggaa gagggcgaaa
     2581 actcggtcaa ttccgggata agtcagtttc cactcatcac gcagggcgag gagcctcttg
     2641 tacgagtagg gatcttccgc cccgagaatt acgttccacg tcaatctagg cttcccagct
     2701 gactgggcaa gacactcgtt gaggtatgcc atggcgtcgc gcggccgcat taacgtgcgc
     2761 ccgaggatga aatcgagtgc tgccccgcgg cgcctgctct tcggcggaag gagatctttg
     2821 atcccgccga tgtgttcccg gcccactttc tctgctgcaa ccttgacgcg ctcatctaat
     2881 agtgccacta gctcgtgctt cgaccacttc atctgaagga caagggctcg gagcttttcc
     2941 tcctgcgcgc cagcgcgacc gaaatcgagc tggctaaaga tgttcgtccg aagcgctacc
     3001 agtaccttta agtttcgaac gcgttgcagg tccagcaccg tgagtagcag gcaccgtatg
     3061 aggtcattga caacccggtg gtcaatccaa tctcggtcaa gatcgtctat gactatgaac
     3121 attcgatttt cctcggaatc gaggatgtcc tcatccagga tatcgatcat ttttgtcagc
     3181 cttgacagct gagtctcatt cacgatcgcc tgaaacttct ttgctagctc gatcctttct
     3241 tcgatcatct ctcccgcgga tgccgtctcg cccgctgcaa tgtgggccac ccccgatgct
     3301 ccaacatctc ctgacctcgt ggcatcaatc ttcttctcga gccttgtcgt aatcgcgcgt
     3361 aagcgcacat cggtgtcgtc ccagaaccga ccctcgaaat cgttgaagta gtcaagagcc
     3421 aattgcttgc tcttatctcg gcgtagccgt tcaaccaacc ctgcccatat accggatttt
     3481 gcctcgtctg aatcgatttt gtaccggcgt cggatgagct ctacgacaag tacgtgtttc
     3541 cacaacgcga tgaagaaggg gtccagatga acctgaagat ccgacaggta cttgaggaca
     3601 ttcgacgtcg agaaatagag gagcgatatg tcaccagggt cgatcttcac gacgccatcg
     3661 ccgttcactt gttgcagatg ccgtaatgcc gccgacttcc cccctccggt ccgcgcaacg
     3721 acaaagcaac gattgtcgcc gtgagaagag atgatgctgt agtgcccgga ttcgtaaaac
     3781 gctgcctcta gaaggctgtc gagctcagct tgttcctcgc cgagactgaa cccgctcctt
     3841 agtcggcctt caggccgcgc cttcgccatc tgactacctc cgatccgagt tcgagccgtc
     3901 ttgtgtgcaa ttcaggccag attgatctct ttgacgtcta gtcaactcgc gatcaactaa
     3961 agtcgcagag cgggcacccc gtctaggggg aagggcgggt ttttccgttc gccgtaagcc
     4021 cttgggggca ggagtcgaag aacgccgcca cctcccgatg acggcttgtc tttcctcctg
     4081 tggagacatc gttaccgctg ggaaacggcc ccgaaacaca cccgaaacac cgctcccgtt
     4141 gcatgaggtc agacaagggg caacggcgcc tgacgctcga agacgaaagg atccggatgg
     4201 acaccggcga caccgccttc atcctcgtgt gctcggccct cgtgatgttc atgaccccgg
     4261 gcctcgccct gttctacggg ggcatggtcc gcagcaagaa cgtcctcggg acgatcatgc
     4321 agagcgtctt cgccctcggg gtcgtgtcgg tcctctgggt cgtcgtcggc tacacgctcg
     4381 ccttcggccc cgacaacggg gggctgatcg ggggtctcga cttcctcggg ttctcggggg
     4441 tgggggccga gccgaacgcc gacctcgccg cgacgatccc ccactcgctg ttcgccgtct
     4501 tccagatgat gttcgccatc atcacgccgg cgctcatcac cggagcgttc gcagagcgca
     4561 tgaagttctc gggttacgtc gccttcaccg ctctgtggct cctcgtcgtc tacgcgcccg
     4621 tcgcgcactg ggtgtgggca ccgggtgggt ggatccgtga gatgggcgcg ctcgacttcg
     4681 ccggcgggac ggtcgtccac atcaacgccg ggatcgcggc cctcgccgcg gcggtcgtcg
     4741 taggcaagcg caggggcttc ggcaaggagg cgttcgtccc gcacaacctg acgatgaccg
     4801 tgctcggcac ggggatcctg tggttcggct ggttcgggtt caacgcggga agcgcgctcg
     4861 gcgccaacgg cctcgcggcg tcggcgttcc tagcgacgaa cctcggcgcg gccgccgggg
     4921 cgtgcgggtg ggcactcgtc gacgcacgca aggagaagaa gtcgacgacg ctcggcgtcg
     4981 cgtccggagc cgtcgcaggt ctcgtcgcga tcacgccggc ggccgggttc gtcggcccgc
     5041 tcgcggcgat cgcgatcggt ctcgccggtg gcgtcgtgtg cgcctatgcg gtccgtctga
     5101 agttccggtt cggctacgac gactcgctcg acgtcgtcgg cgtccacctc gtcggcggca
     5161 tcgtcggcgc cttcctcacc ggcgtcttcg ccgacacggc gatcaactcg gcgggggtcg
     5221 acgggctgct ggccggcggc ggagcggcgt tgctcggcaa gcaggccgtc gccatcgggg
     5281 cgacgctgct gttctcgttc gtcgtcagcg tcgggctgct caagctcgtc gaggcgacga
     5341 tcggtctgcg cgtgaccgag gaagaggagg ccacgggtct cgacctggcg cagcatgcgg
     5401 agtccggcta cgcgttcgcc gagggcggcg gcgggttctc gcccgccccg gccgcggccc
     5461 ccgtccccgc acacgctccc gcggccgtgc gggtcccgag aggaagcgag gcctagatga
     5521 aggtcgtggt cgcagtcgtg aagcccttca agctcgaaga cgtgaaggag gcgctcgaag
     5581 ggatcggcgt gcaggggctg acggtctcgg acgcacgcgg gttcggccgg cagaagggcc
     5641 acaccgaggt ctacaggggg gccgagtacc aggtggactt cttgccgaag acgcgcatcg
     5701 aggtcgcggt cgacgacgac caggtcgacg acgtcgtgaa ggcgatcgtc acctcggccc
     5761 gcacggactc gatcggcgac ggcaagatct gggtcgtgcc ggccgagcag gtcgtgcgga
     5821 tccggaccgg cgagaagggc ccggacgctc tctgaaagac gtaagggcgc cacgtcgcct
     5881 ggggctgagt gacggcacga cggttgccgc ggctcgctca ggcgacgtgg acgccgccct
     5941 gcgcgagctg gccgacgcgg ccctcggccc cgcgggaggc gtgacgctcg tcgccgtcgg
     6001 cggatacggc cggcgcgaga tgtcgccgca ctccgacgta gacctgctgt gcctcgtgcc
     6061 gcggaagccg gacgtctccc ccgcgaccct gcgggggctg ctgtatccgc tgtgggacgc
     6121 ggggttccag gtcggccacg ccgtgcgcac gccggccgaa gccgtcgagc acgcgacgaa
     6181 ggacctagat gccgcgacgt ctcttctgtc ggcccggctg gtggcgggcg acgaggctgc
     6241 gttcgccgag ctcgtcgacc ggcgtgcccg ctggctccgg cgcggggccc gcgcgctcgt
     6301 gcgaacgatc gtctccgcaa ccgaagctcg acattcgtca cgggagcgcg ccggatggtc
     6361 gctcgcgccc gacctgaagg aggacgtggg agggctccgc gacctgcacg ccatgggctg
     6421 gctcgcagcc gccggcggcg agaacacgac ggggctcgac gaggagaggt cggtcctgct
     6481 cgcggcgcgc gaggccctgc acgcgcagtc gaagcgcaag ctcgaccggc tgcggatcga
     6541 cctgcagccc gcggtggcgc gggagctggg gttcgacgat cccgacgagc tgatggaacg
     6601 ggtccactcg agcgcgcgcc gtatcgagag ccggacccgg cgcacgctca acgacctcgc
     6661 ggcgaggggg ccgcgccggt ccgggagcgc gctcgaggtc gcgccgggtg tgcggctcga
     6721 cgacggcgcg ctgggcctca ccgtcccgcc ggatcccgtc accgcgacgt cgctcctcgc
     6781 agcacatgcg acgctcgcca agccgttgtc ggcgcgctct ctggactccc tcgactcggc
     6841 cttcgccggc gaacctcagg agcggtggga cccgcgcctg cgcgacgcgt tcgtacgcct
     6901 gctcggcggg cccgcggcgt cgtccgcgct cgagctcctc gaccacgccg gcgcgtggcc
     6961 ggtgctgctg cccgaatgga gcggcgcgag gggccgggcc caacacgacc cgtaccaccg
     7021 ctacacggtc gacggtcact ccttcgtagc cgttgacgcg gcgaaccgcg tgctcgccga
     7081 ggacggcctc gcgcgcgacg cgggatcgac cgacgcgctg ctggtcgcga cgctgctgca
     7141 cgacgccggc aagggatcgg gtgaggacca ctcggtcgcg ggcgagcgcc tcgcgcgctc
     7201 cgcggcgacg cggatggggt tcgaccatgc gaccgtcgag gagatcgcaa cgctcgtgcg
     7261 atggcatctc cttctggtcg acaccgcgac gcggcgcgac ctcgacgacg gcgcggtgat
     7321 cgaagacgtc gccgcacgcg tcgggacgcc gcgcctgctg cggctgctct acgtgctgac
     7381 ggtggcggac gcgactgcga cggggccgga agcctggacc ccgtggaagg cgtcgctcgt
     7441 gcgcgacctg ttccgcaagg ccctcgtcgc gctcgagacc ggcgcgctcc cggcgcgcag
     7501 cgacgtctcg accaaggcgc gcgaggtcat cgccttcgag cccgcgcttg cgggaagtgc
     7561 agaggacgtc cttacgacgc tcccgccttc ttatctcggg agcgcgaccg tggccgagat
     7621 cgccgacgac atacgcctgt tgctccggcg cccgcgcccc ggagacgtgg tggcgcgcgt
     7681 cgaagacgga accgaggacg gcaccgaagt cctcaccgtg tgcgtggcgg accggcccgg
     7741 ctccctcgcg cgcacggccg gcgtgctggc gatgaacctc gtgacggtgc tgagcgcgca
     7801 ggcgttctcg acgaccgagg gcctcgctct ccagcggttg accgtgcggc cggacgaccc
     7861 ggcgcggatg gagtcggtgg tcgcgggcct cgaggccgcg ttcgccggtc gcatcgcgct
     7921 cgaggcccgg ttgacgaaaa aggcgctcga ctaccgcccc gccggggagg tcgccgtcga
     7981 cgtccgcgtg gtcgacgacg cgtccgtgca ctcgacggtc gtcgaggtac gggggccgga
     8041 cgccctcggc ctgctctacg cgatcgcggc gggcctgagc gacctcgacc tggacatcca
     8101 cgtagcgaag atcgacacgc tgggggcgcg cgtcgtcgac acgttctacg tgcgagatgc
     8161 ccgcgggacc aagccgggac cggaacaaac gaaagagatc gagcgcgcgg tagcccaccg
     8221 cgtctcgagc ctctacggct agcgcgctat ccggttcgcg tacgcgccgt cggtcgctcg
     8281 ctcgaacgcg aggcccatcg cgagcgccag ctcgtcgcgt ccccgcgccg ccgtcagcgt
     8341 caggttcgta gtcagcccgc tcggcatgac gccgcacggc agcgacagcg cgggcacgcc
     8401 tccgaggttc atcggcgcgt tcgtgcggag gtacgcgagc tcggccgcgg ccgggccgga
     8461 cggaagctcg accagcaggg tgtcgacggg ggcagggggg ccgggaagcg tcggcgtcac
     8521 gacgacgtcg acctcttcga agacgcgggc ccagtccgcg gccatcgctt cctgcgtccc
     8581 ggctgcctgg cggcggtcgt cttcggtcgc ggacctgccg agcgcgatca ggtgcgcggt
     8641 cggcggctgg aacgcgccgt cgtcgacgcc ggagaggcgc tcggcgaaca gcggcagcag
     8701 gagcgcgggg accgcgaacg aagccgtcgc cgccgccggg aggtcgacct cacgcacgtc
     8761 ggcagcgagc ccgcgcagga cttcgaggcc cgactcgacc gcggccccca cacccgggtc
     8821 gaggtcgtcg aagaagaacg acgacgggat cccgagccgg agcgactctc cctccggcag
     8881 cgacagctcc tcgtcggcca tgacgccgaa cagcaacgcg acgtcctcga ccgagcgcgc
     8941 catcgggccg accccgtcga tcgcgccccc gtctcccacg aaccccttcg tcggcagccg
     9001 gccccccggc gccttcagcc cgggcgtccc acagcacgcg gacggacacc tgatcgagcc
     9061 tccggcgtcg gtccctatgg cggcggggac gatgccggcg gcgagagcgg ccgccgaccc
     9121 cccgctcgag cccccgggca cgagggccgg gtcccacggg ttgcggatcg ggccggtggc
     9181 ggtgacggcc gaggtcatcc cgacgcccca ctcgtggagg ttcgtgtagc cgacgacgac
     9241 ggcccccgcc cgccgcaggc gctcgacgat cgtggcggtc ccggtgagcc aggtcccgtg
     9301 cgcgttcgac ccgcacgtcg gcacgcggcc ctcgtccacg aacatgtcct tgaccgccag
     9361 ggggactccg gcgagggggc ccgaggcacg ccctgcctcg tccacggcct ccgcccgcgt
     9421 ctcgacgaac gcgttcagct cctgggaacc ggcccgatcg agcgcggcag cggcgacctc
     9481 gcgcgcgtcc agctccccgc gcttcagggc gcggcgaagc gcgccgatac cgagcgacag
     9541 cgggtcttcc acaggccggg ttctacaccc cgaacggaac aggaaaaaag tgggtgaaga
     9601 gctctcaagg taatcggagc tcctgccgaa gttagaggca cacggcaaca cccaagcgaa
     9661 aacggcaaac ccgtcgcaag gcgggggcgc aaagccaggg gccccacgag ggcggcccag
     9721 ctaccgaact gggaggttcg ctatgaacgc tttcaatcgt gcagtcatcg ggttcaccgc
     9781 aacggctctc ttcgtgggcg cagcgcccgc aatcaccgca ggtgcgcaga ccaagcaggg
     9841 tggctgctgg gacgtcaagg gcgccgagcg tggtttcgcc aagcgcatca acgacgtccg
     9901 aggggtcgcc cgtgcaggga ccctccggct cgaccccgag ctctcccgtg cagcccgcct
     9961 ccacacccgg gagatggtga agcagcagaa gctgtaccac acgccttccg acaagctcag
    10021 caagcgggtc acgaactgga ccgtcctcgg cgagaacgtc ggcgtcggtg gcgacgtcca
    10081 gagcctgcac gacgccttca tggcctcgcc gggtcacgcc aagaacgtcc tctacgactc
    10141 cttccgccac gtgggcatcg gcaccatcaa gtcccgcggc cagctctggg tcacggtgat
    10201 cttcgaggcc gagaccaacc ccggcaccac gctcaacatg ccgagctgct gacgaccatc
    10261 tccggactgg gaacctagaa gcaccaccca aacgacctac gcgacccggc tcttctccca
    10321 gcgagccgac cgagacgaag gccccgaact cccagcgggg ccttcgtcgc gtctgtaaag
    10381 aagtgagcgg acggctcgct ttgccggtgc gatccttgtc ccgtgcgggc gctccgaacc
    10441 catgtgacag tcgtggtcgc ggcggcgagc ctcccaatcg ttgcgccttc ggccgccgcc
    10501 gtgaccccct gtttcggcga gaccggcgtg agaagggctc tcaggccccc acggtgaaga
    10561 acacctcggg gctcggcgcc ttcgtgtggt cggcgtcgcc ctcgaaccac gtgatggccc
    10621 ggtagcggtt gccgggcgcc gtgttcggca gcgcgacctc ggtcgtgtac gagcccgact
    10681 cgtcgagccg ccggatccgc ttcttcagca gcgtcatcgt gccttcgact gagacctgcc
    10741 acacccgcac gctcactcgc ttgccggcgt gcggcggcgc gacgttgccg gtgaggcggg
    10801 ccgaggtcgt ccccgccggg tagtggtagg tcccgaacgc gtcggcgatc gctccctcgg
    10861 gcaccagcgt cacctccggc tggacgcgga tcctgatctg gcggctctgg ctcccccacg
    10921 tcgtgtcgtt gccgtcgtag acgacgaacg ccttcgtgtc cgcctgcggc gcgaccgaga
    10981 acgcgaacga cccgtcggcc cccgtcgtcg tcgtcccggc gggggtccac ccgcggcccc
    11041 gctcgttcgt gtagaggacg acggactgga acgcgagcgg cgacccgtcc tcgagcgtca
    11101 gggtcccggc gaccgccgag gactcgccgt aggtgacgcg ccgcggctca gcgcctgcgt
    11161 tcatcacgga cgtcgtcttg ccggccccca tggccgcggc cgggtccttg cggatcgccg
    11221 ggagcgcggc gt
//
LOCUS       DASQDO010000512         1866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_788371, whole genome shotgun sequence.
ACCESSION   DASQDO010000512 DASQDO010000000
VERSION     DASQDO010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788371"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1663)
                     /locus_tag="VEV43_14035"
     CDS             complement(239..1663)
                     /locus_tag="VEV43_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYP24686.1"
                     /translation="MRPLLAFVTCLFVFAPPASAAPEPELWLTPAVDGPVSVRFEAPV
                     RKWGSGHRGIDYAVAPGTAVRAAAAGTVTFAGSVAGTRAVTIDHGPFQTTYSALSSVD
                     VAIGQVVDRGHWIGAAGEAHPGSGTGLHLGVKNGDEYLDPALFFGPVDASAAIHLAPV
                     VDRAVEDIPDSFHPFPLSAGDHERTCVEHDSVDAAVTSPNDNVVVAINGLASRAEGPT
                     GTALYRHAVELLRYPPERVYTFSYAGMQGPRLHEPYGQSATYGDLNVAATKLHALLRE
                     IGTRHPGAEVDLLAHSQGGLVARAFLQQLETSWVPGLPHVDHLVTLATPHTGAPAADM
                     ARRMDGTLVGNAINDVVSGLSRDTGWFPDPRSQAVSQLSPSSELHGVLAREDVAFGTR
                     VLSLSVPDDFVVPAHRARLEHEQNLTLPPSGGFAHSRIVESERAASVAHDFLRDAAPG
                     CAGGWDNWGARAGKLIEFVEDAIW"
BASE COUNT          283 a          675 c          650 g          258 t
ORIGIN      
        1 cgcgtcgaaa ggcaggcgat ttgtggccat ggggccatcc tagggggcag ggtgtccgga
       61 gggccggttt gtacctcgcg cacaattggg gcttggtttc tctgccccgc cagcggttga
      121 actgggcccg ggccccgagg agaatatgtc ccatacacaa cgatccaccc ggccccctcc
      181 gttaccccac ccatcccccc gacggagggg gcttcttcat ttccagggtc cggcggcgct
      241 accagatcgc gtcctcgacg aactcgatca gctttccggc ccgcgccccc cagttgtccc
      301 agcccccggc gcaccccggc gcggcgtcgc gcaggaagtc gtgcgccacg gacgccgccc
      361 gctcggactc gacgatccgg ctgtgggcga agccgccgga cggcgggagc gtcaggttct
      421 gctcgtgctc cagtcgcgca cgatgcgccg gtacgacgaa gtcgtcgggt accgacaacg
      481 acagcacacg agtgccgaac gcgacgtctt cgcgcgccag taccccgtgg agctcggagg
      541 acggcgacag ctgcgacacc gcctgtgacc gcgggtccgg gaaccacccc gtgtcgcgcg
      601 agagtcccga cacgacgtcg ttgatcgcgt tgccgaccaa agttccgtcc atccggcgcg
      661 ccatgtcggc tgcgggcgcg ccggtgtgcg gcgtcgccag cgtcacgagg tggtcgacgt
      721 gcggaaggcc cggcacccac gacgtctcga gctgttgcag gaacgcccgc gcgacgagac
      781 cgccttgcga atgcgcgagc aggtccactt ccgctccggg gtggcgcgtc ccgatctcgc
      841 gcagcagcgc gtggagcttc gtcgcagcta cgttcaggtc gccgtaggtg gcggactggc
      901 cgtacggctc gtgcaggcgc ggcccctgca tccccgcgta cgagaacgtg tagacgcgct
      961 cgggcggata tcgcaggagc tcgaccgcat ggcggtacaa cgcggtgccg gtcggacctt
     1021 cggcccggct cgccagcccg ttgatcgcga cgacgacgtt gtcgttcggc gaggtcaccg
     1081 cggcgtcgac cgaatcgtgc tcgacgcaag ttcgctcgtg gtcgcccgcc gacagcggga
     1141 agggatggaa cgagtcgggg atgtcctcga cggcgcggtc gaccaccggc gcgaggtgga
     1201 tcgcagccga cgcgtcgacg gggccgaaga agagcgcggg gtcgaggtac tcgtcgccgt
     1261 tctttacccc caggtggagg cccgtcccgc tcccgggatg cgcctcgccc gccgccccga
     1321 tccagtgtcc gcggtcgacg acctggccga tcgccacgtc caccgaggag agcgccgagt
     1381 acgtcgtctg gaaggggccg tggtcgatcg tcaccgcccg cgtgcccgcc acagagccgg
     1441 cgaacgtcac cgtgccggcc gcggcggcgc ggacggcggt gccgggagcg accgcgtagt
     1501 cgatgccgcg gtggccgctc ccccacttgc gcaccggcgc ctcgaaccgg accgagaccg
     1561 ggccgtcgac cgcaggggtg agccagagct ccggctcggg ggccgcggac gcgggcggag
     1621 cgaacacgaa caggcaggtg acgaacgcaa ggagaggtct catgcgcccg agcgtcggcc
     1681 accgagcgcc tcgccgcagt gacccgcgcc actacgggac gatcaaagtc cccgctctcc
     1741 cgtagacgcc gtcctcgcag cactcgatcg aagtgatcac gcctgtcccg ccggaggact
     1801 cgacgaagcg gcacaccgcc tcgaccttcg gccccatgct ccccggcggg aactcgcccg
     1861 cgtcga
//
LOCUS       DASQDO010000513         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_910482, whole genome shotgun sequence.
ACCESSION   DASQDO010000513 DASQDO010000000
VERSION     DASQDO010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910482"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VEV43_14040"
     CDS             complement(<1..957)
                     /locus_tag="VEV43_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24687.1"
                     /translation="MTRIGRIALPLLLAASSLAPPAGASPIVADAAGAGLEPVASVEY
                     PDGMHLAVTRIKGRDYVFASASTVTPGGGIRVFDVTKPARPKIVATIPCSGGQGFLDV
                     SHDRKTLIVGEGRAHPADPCMPGEAMGIYTVDISNPRKPKPLGYALADRAAHHLDAHP
                     TKPFVYVAHGDPALTAADAFGAYEVWSIANPAKPRLVTTAKVTGYHGPHDIAFNADGT
                     RAVASSMSAIQVLDTSDPADPKELQVLQCPGCSHNHEAHVTPDQKHVVVSDETTGGIV
                     SPCPLGALYFSAWNADGPEHMTYVGEWQPAEAGTPSGAPTNAP"
     gene            1044..1475
                     /locus_tag="VEV43_14045"
     CDS             1044..1475
                     /locus_tag="VEV43_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24688.1"
                     /translation="MEPRIDVITIAVADLDRALAFYRDGLGLETEGIIGTEFPGSDTE
                     PAGAAVMFRLPRGLTFALYPRTELAKDANVAAPEPSTGGFSVGWFVATDQEVDEVLAR
                     AEAAGATLTDTAHRRPWDLYSGYFRDLDGHLWEVISHPDAR"
     gene            complement(1639..2406)
                     /locus_tag="VEV43_14050"
     CDS             complement(1639..2406)
                     /locus_tag="VEV43_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24689.1"
                     /translation="MKILRVGALCVAVLLTAAACGGDDGDDAAAPTAAAEETAAGSDE
                     EFCDAVVAAETEVLAATSGGDPSGVEDLLVTVEETAPAELEEQVGVVVATVRDALETR
                     NDRAFQSEEFSKNEEEVDQWVAGNCGFERIDVTAVDYAFEGVPETIPAGTVTFGFSNE
                     GDELHEMLTVRYKDPDTTIEDLMKLSDKEAEQAIDFLGASFGPPGFSDTESRELTPGK
                     YALVCFVPVGSTSEKAARKADGPPHVARGMSAEFTVE"
     gene            2547..>3441
                     /locus_tag="VEV43_14055"
     CDS             2547..>3441
                     /locus_tag="VEV43_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24690.1"
                     /translation="MRKTVIAVAAALAAAGVAAPAHAAVKCFGRSPTIVGSKGADTLR
                     GTNGDDVIVSLAGKDRVFAGKGKDRVCTGGGRDLVVGEGGRDQIDAGGGADRIYGASQ
                     RKYDPAGEHNPGNAETPEVLAGGPGADVIRGGGGNDVLLGGPGNDRLFGEDEGDRIDG
                     DDGADLIDTGGTNERVSYDFALGGPGDDRIVSLDAGPDELKGADGDDRIVAATGSRDD
                     AHGGEGDDHLEGALVSFEAATRGVTVDLVTETATGQGTDVVVDAEGVEGSGFDDVLLG
                     TEGGDIMRAGAGDDVVDGRGGD"
BASE COUNT          495 a         1183 c         1264 g          499 t
ORIGIN      
        1 cggcgcgttc gtcggcgccc ccgacggcgt gcccgcctcg gccggctgcc actcgccgac
       61 gtacgtcatg tgctccgggc cgtccgcgtt ccacgcggag aagtacagcg cgcccagcgg
      121 gcacggcgac acgatgccgc ccgtggtctc gtcgctgacc acgacgtgct tctggtccgg
      181 cgtgacgtgg gcctcgtggt tgtgcgagca ccccgggcac tggagcacct ggagctcctt
      241 cgggtcggcc gggtcgctcg tgtcgagcac ctggatcgcc gacatcgacg acgcgacggc
      301 ccgcgtcccg tccgcgttga acgcgatgtc gtgggggccg tggtagccgg tgaccttggc
      361 ggtcgtcacg agcctcggct tggccgggtt cgctatcgac cagacctcgt aggcaccgaa
      421 cgcgtccgcg gccgtgaggg ccgggtcgcc gtgcgcgacg tagacgaacg gcttcgtcgg
      481 atgcgcgtcg aggtgatgag ccgctcggtc ggcgagcgcg tacccgaggg gcttcggctt
      541 gcgggggttc gagatgtcga cggtgtagat gcccatcgcc tccccgggca tgcaggggtc
      601 cgccgggtga gcccggccct cgccgacgat cagggtcttg cggtcgtgcg agacgtcgag
      661 gaagccctgg cctccgctgc acgggatcgt cgcgacgatc ttcgggcggg cgggcttcgt
      721 gacgtcgaag acgcggatgc cgccaccggg ggtcaccgtc gacgcggagg cgaagacgta
      781 gtcgcggccc tttatccgtg tcacggccag gtgcatgccg tccgggtact cgacggaggc
      841 gacgggctcg agcccggccc cggccgcgtc ggcgacgatc gggctcgcgc ccgcaggggg
      901 cgctaacgac gaagcggcca gaagcaacgg gagagcgata cgtcctatac gcgtcatgga
      961 gattgggttc gccgggcggt tttcccaccc tgcatcatgc cgggtcgggc ccgtgccaat
     1021 aagggcacgg tacggtcgca gccgtggagc cccgcatcga cgtgatcacg atcgcagtcg
     1081 cagacctcga ccgcgcgctc gccttctatc gcgacgggct cgggctcgag accgagggaa
     1141 tcatcggcac cgagttcccc ggcagcgaca ccgagccggc gggcgccgcc gtgatgttcc
     1201 gcctcccgcg ggggctcacg ttcgctctgt acccgaggac cgagctcgcc aaggatgcga
     1261 acgtcgcggc ccccgaaccg tcgacgggcg gcttcagcgt cggctggttc gtggcgacgg
     1321 accaggaggt ggacgaggtg ctcgcccgcg ccgaggccgc cggcgccacc ttgaccgaca
     1381 ccgcgcaccg gcggccctgg gatctctact cgggctactt ccgcgacctg gacgggcacc
     1441 tctgggaggt catctcgcac cccgacgcgc gctaggccta atcacctacg cgatcgatag
     1501 ccgcgcgaca ggtgggatca agggccgtgg tgcgcgtggt tggcgtggta cgtgtggttg
     1561 cacttccaac ctcgccaacc acacgtacca gacgtaccag cgcctccgga agaaagggtg
     1621 cgacggccgc tagggccgct actcgacggt gaactcggcc gacatcccgc gcgccacgtg
     1681 cgggggcccg tcggccttgc gtgcggcctt ctcgctggtc gacccgacgg ggacgaagca
     1741 cacgagcgcg tacttgcccg gcgtgagctc cctgctctcg gtgtcgctga acccgggggg
     1801 gccgaacgac gcgccgagga agtcgatcgc ctgctccgcc tccttgtcgg agagcttcat
     1861 gaggtcctcg atcgtggtgt cggggtcctt gtagcggacg gtcagcatct cgtgcagctc
     1921 gtcgccctcg ttcgagaagc cgaacgtcac ggtcccggcg ggaatggtct ccgggacgcc
     1981 ctcgaacgcg tagtcgaccg cggtcacgtc gatgcgctcg aagccgcagt tgccggcgac
     2041 ccactgatcg acctcttcct cgttcttcga gaactcctcg gactggaagg cgcggtcgtt
     2101 gcgggtttcg agcgcgtccc gcaccgtggc gacgacgacc ccgacctgct cctcgagctc
     2161 ggccggcgcc gtctcctcga cggtcacgag caggtcctcg acgccgctcg ggtcaccgcc
     2221 cgaagtcgcg gcgagcacct cggtctctgc ggccacgact gcatcgcaga actcctcgtc
     2281 ggagccggcg gcggtctcct cggcggcggc cgtcggggcg gcggcgtcgt ccccgtcgtc
     2341 tcctccacag gccgcggctg tcagcagaac ggcgacgcag agcgcaccga cgcgcaggat
     2401 cttcaagacg tcacttcccc tcgtggtggc ggttctgggc ggcgcgcact ctaaccgtgt
     2461 gctcaaacct gagcggacgg cgggggccgg ggtccttacg gtgggaggga ggggtaatac
     2521 cggaataggt gttgacagga ggatccatgc gcaagaccgt catcgcggtc gccgcggcac
     2581 tcgccgcggc cggcgtcgcc gcgcccgccc atgcagccgt gaagtgcttc ggccgctcgc
     2641 ccaccatcgt cgggtcgaag ggcgccgaca cgctcagggg tacgaacgga gacgacgtca
     2701 tcgtctcgct cgcggggaag gaccgcgtct tcgccgggaa gggcaaggac cgcgtctgca
     2761 cgggcggggg ccgcgacctc gtcgtgggcg aaggagggcg cgaccagatc gacgccggcg
     2821 gaggggccga ccgcatctac ggggcctccc agcgcaagta cgaccctgcc ggagagcaca
     2881 accccggcaa cgccgagacc ccggaggtcc tcgccggtgg ccccggcgcg gacgtgatcc
     2941 ggggcggcgg cggcaacgac gtcctcctcg ggggccccgg gaacgaccgg ctgttcggcg
     3001 aggacgaggg cgatcgcatc gacggcgacg acggcgcgga cctgatcgac acgggcggca
     3061 cgaacgagcg tgtgtcctac gacttcgcgc taggcggtcc gggcgacgac cggatcgtgt
     3121 ccctcgacgc cgggccggac gagctgaagg gcgccgacgg cgacgaccgc atcgtcgccg
     3181 cgacgggctc ccgcgacgac gcccacggtg gggagggcga cgaccacctc gagggcgcgc
     3241 tcgtgtcctt cgaggccgct actcgcggcg tgaccgtcga cctcgtgacg gagaccgcga
     3301 cggggcaggg aaccgacgtg gtcgtcgacg cggagggggt cgagggatcg ggcttcgacg
     3361 acgtgctcct ggggacggaa ggcggcgaca tcatgagggc cggcgccggt gacgacgtcg
     3421 tcgacgggcg cggcggagac g
//
LOCUS       DASQDO010000514         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_727048, whole genome shotgun sequence.
ACCESSION   DASQDO010000514 DASQDO010000000
VERSION     DASQDO010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727048"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1043)
                     /locus_tag="VEV43_14060"
                     /pseudo
     CDS             complement(1..1043)
                     /locus_tag="VEV43_14060"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
     gene            complement(1040..1990)
                     /gene="gnd"
                     /locus_tag="VEV43_14065"
     CDS             complement(1040..1990)
                     /gene="gnd"
                     /locus_tag="VEV43_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYP24691.1"
                     /translation="MTQIGFVGLGRMGGNMVARIKRDSDHDVVAFDRDPETVQRIADA
                     TGATGASSLEDLVSKLDAPRMVWIMVPAGGPTQGTVDTLAGLLDAGDMIIDGGNSKWT
                     DDRERAAALSERDIEYADVGVSGGVWGLQVGYCMMVGGPDAAVQRLAPILDVLAPETS
                     EASRGVLGDRGWGHFGPSGAGHYVKMVHNGVEYGLMQAYAEGFDLFDKCDFELDNAKI
                     AHLWNQGSVVRSWLCELAALAFEQEGNTLAGLSGYTADSGEGRWTIEDAMAIDVPTSV
                     LTASLHARFDSRGNGDFTHRMLAALRAQFGGHATKKSADG"
     gene            2154..2348
                     /locus_tag="VEV43_14070"
     CDS             2154..2348
                     /locus_tag="VEV43_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24692.1"
                     /translation="MASSSDDEPTTQQLRAVQSDREVDERERAKDAPTDTEQLAAVRR
                     ADKAAYLKEKLDEKAESERE"
     gene            2345..>2653
                     /locus_tag="VEV43_14075"
     CDS             2345..>2653
                     /locus_tag="VEV43_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYP24693.1"
                     /translation="MSELPEHVARNRADWDAHAKDWVEPGRRDWAREEPWWGIFTVPE
                     SQLHVLPDELDGLDTIELGCGTGNVSEWLARRGARPVGNDNSEAQLATARLLQAEFGI
                     D"
BASE COUNT          385 a          927 c          953 g          388 t
ORIGIN      
        1 gtctcggtgg tggagtcgtc gggcacgccg gcctcgtcga ggtagcccgg cgccgcgtcg
       61 ccggcgacga tccccggcgc gtactggccg cgcaccgcca tctgcgggat ctcgacgggc
      121 gtcggcgcgc tgatcgcgag cagcaccatg accttctcgt cgcgcaccgg gtcggcctcg
      181 aaggcgacct gctgctacat gcacagcagc gacagcagct gcagcctgtg gttctggacg
      241 aggtcgcgca gcgctccggc ggagtcgtag tagcccgccc gcgagccgat gccgatgtcc
      301 tcggacgccg tgatctgcac gtggtcgatg tagttgcggt tccacagcgg ctcgaacatg
      361 ttgttcgcga agcgcagcgc caggacgttc tggacggtct ccttgccgag gtagtggtcg
      421 atgcgaaaga cctgctcctc gtggaagacc gacagcaccc gcgcgttgag ctcgcgcgcc
      481 tcctgcagcg agcgcccgaa cggcttctcg atgatcacgc gcacctcggc ggcctcgcga
      541 tcggcgagct cgtggcggcc taggcagtcg acgatgacgg gaaagaacgt cggcgcggtc
      601 gagaggtaga agcagcggtt cagcaacagc ccgacctcgg cgtcgagcca gtcgagctcg
      661 cgccccagcg cgtcgtagac cgcgtcgtcg tcgaacgagc ccgtgacgta gcgcacgtcg
      721 gagaagagct tctcgagcac cttctcgtcg ggtgggcggc gcgagaactc ccgcacggac
      781 tcgagcgcca tcgcgcgaaa ctcgtcgtcg gtcatctccg agcgggcgtt gccgaccagg
      841 ccgaagcgct cgggcagcgc gccctcgtgg gcgaggttgt agagcgcggg cagcagcttg
      901 cgccgcgcga ggtcgccggt cgcgccgaag atcacgagcg cggtcgggtg gaccgggagc
      961 cgctcgagcc cgtcggtgag cgggttgtcc tcggggccgg tctgcgcgcc gggggcggtc
     1021 gcggcgcggt cggtcgcgct cacccgtcgg cgctcttctt cgtcgcgtgg ccgccgaact
     1081 gcgcgcgcag cgccgcgagc atccggtgcg tgaagtcgcc gttgccacgc gagtcgaagc
     1141 gcgcgtgcag cgacgccgtg aggacggacg tcggtacgtc aatggccatc gcgtcctcga
     1201 tcgtccagcg cccttctccg gagtcggcgg tgtagccgct caggcccgcc agcgtgttgc
     1261 cctcctgctc gaaggccagc gcggccagct cgcacagcca cgagcgcacg accgacccct
     1321 ggttccacag gtgcgcgatc ttcgcgttgt cgagctcgaa gtcgcacttg tcgaagaggt
     1381 cgaagccctc cgcgtaggcc tgcatgagcc cgtactcgac gccgttgtgg accatcttca
     1441 cgtagtggcc ggcgccgctc gggccgaagt gcccccagcc gcgatcgccg aggacgcccc
     1501 ggctcgcctc gctcgtctcg ggcgcgagca cgtcgaggat cggcgcgagc cgctggaccg
     1561 ctgcgtcagg gccgccgacc atcatgcagt agccgacctg cagcccccag acgccgccgc
     1621 tgacgccgac gtccgcgtac tcgatgtcgc gctcgctcag cgcggccgcc cgctcgcggt
     1681 cgtcggtcca cttcgagttg ccgccgtcga tgatcatgtc gccggcgtcg aggaggccgg
     1741 cgagcgtgtc gacggttccc tgcgtcgggc cgcccgccgg caccatgatc cacaccatcc
     1801 gcggcgcgtc gagcttgctc acgaggtcct cgagcgaact cgcgccggtc gcgccggtcg
     1861 cgtcggcgat ccgctgcacg gtctcggggt cgcggtcgaa ggcgacgacg tcgtggtctg
     1921 agtcgcgctt gatgcgggcg accatgttgc cgcccatccg gccgaggccg acgaagccga
     1981 tctgagtcac aggttgtctc cgcgcgtcgt gggcgtgcgt ggttggttga gcatatgccg
     2041 acgttgtgcc gggggtgaag tcgctgcgct ctctacccag gctcgcgcaa cggcaaatcg
     2101 ccaggacgtc ggcgacgcga gcacgggtga ccacgtcgag gtagcctcgc gcgatggcct
     2161 cctcatccga cgacgagccg accacccagc agctgcgcgc cgtgcagtcc gaccgcgagg
     2221 tcgatgagcg cgagcgcgcg aaggacgccc cgaccgacac cgagcagctc gctgccgtgc
     2281 ggcgcgccga caaagcggcc tacctcaagg agaagctcga cgagaaggcc gagagcgaac
     2341 gcgagtgagc gagctgcccg agcacgtcgc gcgcaaccgg gccgactggg acgcgcacgc
     2401 gaaggactgg gtcgagcccg gccggcgcga ctgggcgcgc gaggagccgt ggtggggcat
     2461 cttcaccgtc cccgagtcgc agctgcacgt cctgcccgac gagctcgacg gtctcgacac
     2521 gatcgagctg ggctgcggca cgggcaacgt ctcggagtgg ctggcgcggc gcggcgcgcg
     2581 gccggtcggg aacgacaact ccgaagccca gctcgcgacg gcccgcctgc tgcaggcgga
     2641 gttcgggatc gac
//
LOCUS       DASQDO010000515         6987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1216433, whole genome shotgun sequence.
ACCESSION   DASQDO010000515 DASQDO010000000
VERSION     DASQDO010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216433"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VEV43_14080"
     CDS             complement(<1..937)
                     /locus_tag="VEV43_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYP24694.1"
                     /translation="MAFSPTIDELRRAVDEALAADLRRRAEELPEARFLIDEMWRLTA
                     AGGKRLRPAFCYWGYRVAGGEHGDAILRAASSLELLHTFAIVHDDIMDAADERRGEEA
                     VHARHGVGVGILVGDLALVLADDAFMGAGFPAEATARAFAAYSRMRQEVIAGQYLDLT
                     YAARSDITVEDARRVAVLKSGRYSIEEPLAIGAALAGAPDDFVARLAAFGGPLGEAFQ
                     LRDDLLGTFGDRSHVGKPVDSDIVEGKRNVLYAFAAAATAGEEHAVLASRWGGDSLTP
                     DEIARLRALLESSGARAATEALLDEQVAVARERLDD"
     gene            955..1308
                     /locus_tag="VEV43_14085"
     CDS             955..1308
                     /locus_tag="VEV43_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24695.1"
                     /translation="MRKLIVVLVLSGLALLGAACGGDEAEEPGGGGEPVAAEDAPDEV
                     TGVLLDVESEGVGEITSFTLKEGDDTYEIFIAGDVDYGFNLGHLNEHLTTGDPVHVPL
                     EVRDGKLYAVSIDDA"
     gene            complement(1305..2216)
                     /locus_tag="VEV43_14090"
     CDS             complement(1305..2216)
                     /locus_tag="VEV43_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYP24696.1"
                     /translation="MKRRLCLGAIAALLALTASACGGSDDGGGSGGSDKSDSASSGAE
                     SFEQPFDDVKAYPVFASSEVVEGENRFLIGLLNDEDAPISSPDISMHVAFFDLAESAE
                     EPAFETDAEFIDTGPRGLYVTYPTFDSAGRWGAEVTVSGDGLDETVRAGFDVGKDGTT
                     PAIGEPAPASKTLTASGRKLEKITTDTKPDPDFYESSIHEAVEKGDPFVVTFATPKFC
                     TSAVCAPTLDIVKKESKDWPQVEFIHVEVYENLDDPSNLKPVPAVKEWGLPSEPWVFV
                     VDAKGKVAAKFEGTVSPQELKEALADV"
     gene            2275..3210
                     /locus_tag="VEV43_14095"
     CDS             2275..3210
                     /locus_tag="VEV43_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HYP24697.1"
                     /translation="MTLFRRLTSASLIATIVLIAIGGLVRATKSGLGCGTDWPDCGGR
                     LVPALETRAEIIEFSHRFTAAIVGFLILALAIVAVRRLRDERRIKVASVAALVLVVFQ
                     ALLGAAVVKAELERLVVVAHLVMAMSLLALLVYLAGMVAARTRGLDGATDARLSRRIA
                     LAGLGVLALLVVGSLASTRDIGPSWPLVNGELVPDLGVETFALHWFHRVLAGIVGIAV
                     FAVAWSVIKRRDEMPAAARYAHAAAGVFGFEIFVGALNLWTDLNALVVALHLTTGALI
                     WASFAAMVTVTHPSVRAASEASVARGVPAMAAGGR"
     gene            3212..4177
                     /locus_tag="VEV43_14100"
     CDS             3212..4177
                     /locus_tag="VEV43_14100"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HYP24698.1"
                     /translation="MKRLVIGEPTGTLTVEEVVHAPVAEVEVTAPATFRQRAAAYFAL
                     TKPRIVELLLVTTVPSMVVAAQGWPSWLLVLNTLIGGALSAGGANAINCYLDRDIDRV
                     MPRTKRRPLPMHQVEPARALAFGVILGVVAFAYLWATVNLISAVLTTVALLFYVFVYT
                     IGMKRSTPQNIVIGGAAGAFPALVGWSAVTGTVELPALALFGIIFYWTPPHFWALAMR
                     YEKDYAAAGVPMMPVVYGREETAKHVLLYSFLLLAMSLVFFSVGRMGAIYLVSALVLN
                     GVFLYGAIRLYRNTAPTVAWWLFRFSIYYLALLFGAMALDRLLLA"
     gene            4328..6931
                     /locus_tag="VEV43_14105"
     CDS             4328..6931
                     /locus_tag="VEV43_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYP24699.1"
                     /translation="MSRRLFALSLLGALLLTSLPVSIAAQEQGPPKVPDTMEAYSIVP
                     PGQDGNVTPDEVPSGDFGPHFDDQRELYASLIDDEDVTDDEIATYFHSMQFGPKEVER
                     DYQPVEGVTVYRDEFGIPHIYADSFDTASHALGYTTAEDRLWEMDVFRHAAKGELSTL
                     IGPDYLPMDVTARREGYTEEEVQKMYDDLDDKFGATGKTVQDGLQAYADGVNQYIAEL
                     RTNPAQCPAEYQALGNPCPEPEPADWTPLDTLYVAILQLRVFGETAGTELDNAGLYAH
                     LVEKHGSKLGPKIYEDFVSRNDRRSATSIPAREGRFPSQALGKTKKASFAIPDDAPAL
                     ARQTSREQKRVQDFLASMGFRAPASNALIVGAKESASGNPMQIGAPQVGYAVPSFFWD
                     VDVHVSGEDEAHFRGPAVPGASALVPLGRGPDYAWTLTTGYSDAVDTKVELLCDPAGG
                     EAAQDSNGYMFEGECREMESRSETFVVKPSAGSPGPPSVEQRTFYRTVHGPVFARGEV
                     GGKPVAFVKQRFFWKREVDSIPQFYKWNTQVDSIEDFAKAAGKFTMSFNTFYADDRDI
                     GYFHVGYYPVRPKGYHPSLPTWGTGDWEWKGRLPYSRHPKVINPKQGWIANWNNKPAT
                     RWDNYDGIKWGSIHRVELLQNEMHRHFDGKRKGRLSDLVDVIRQAATRDARAVYLGRK
                     MTRWGGAAEAAEADQALKLVRAWVKGGAHRHNRDWKDGDTAEDNGPAAAIFDAWFDAL
                     VHRVFDDEIGADAYDLVGTPVTDRDMWHDFSSYLDNLFSKRAARGYARNYCDNLDTPA
                     KEGCRAQVAAALDDALAALKEAQGEDVSAWTTDAWMTEFESLGLGSADPIPWQNRGTH
                     NHVVEILRKAR"
BASE COUNT         1049 a         2434 c         2430 g         1074 t
ORIGIN      
        1 ggtcgtcgag acgctcccgg gcgaccgcta cctgctcgtc caggagggcc tcggtcgccg
       61 cgcgcgcccc cgacgactcg aggagcgcgc gcaggcgtgc gatctcgtcg ggcgtcaggg
      121 agtcgccgcc ccagcgggac gcgaggaccg catgctcctc gcccgcggtg gccgcggccg
      181 cgaacgcgta gaggacgttg cgctttccct cgacgatgtc ggagtcgacg ggcttgccga
      241 cgtgcgagcg gtcgccgaac gtgccgagca ggtcgtcgcg cagctggaac gcctcgccga
      301 gggggccgcc gaacgcggcg agccgcgcga cgaagtcgtc gggcgcgccg gcgagcgcgg
      361 ccccgatcgc gagcggctct tcgatcgagt agcggcctga cttcaggact gcgaccctgc
      421 gcgcgtcctc gacggtgatg tcgctgcgcg ccgcgtacgt gaggtcgagg tactggccgg
      481 cgatcacctc ctgcctcatg cgtgagtacg cggcgaaagc gcgggccgtg gcctccgccg
      541 ggaacccggc ccccatgaac gcgtcgtcgg cgagcacgag cgcgaggtcg ccgacgagga
      601 tcccgacgcc gacgccgtgg cgcgcgtgca cggcttcttc cccccgccgc tcgtcggccg
      661 cgtccatgat gtcgtcgtgc acgatcgcga acgtgtggag gagctcgagg ctcgacgcgg
      721 cccgcaagat ggcgtcgccg tgttcgcctc cggcgacgcg atagccccag tagcagaagg
      781 ccgggcgcag ccgcttgccc ccggccgcgg tgaggcgcca catctcgtcg atcaggaagc
      841 gagcctcggg aagctcttcc gcgcggcggc ggaggtccgc tgcgagggcc tcgtccacgg
      901 cccgccgcag ttcgtcgatg gtgggcgaga aggccatacg gctaccctag ggcgatgcgg
      961 aagctgatcg tcgtcctcgt cttgtccggt ctcgcgctgc tgggggccgc gtgcggaggc
     1021 gacgaggccg aggagcccgg gggcggaggc gagcccgtcg cggccgagga cgcgcccgac
     1081 gaggtgacgg gggtcctgct cgacgtcgag tcggagggcg tcggcgagat cacgtcgttc
     1141 acgctgaagg agggcgacga cacctacgag atcttcatcg ccggggacgt cgactacggc
     1201 ttcaacctcg gccacctcaa cgagcacctg acgacgggtg acccggtgca cgtgccgctg
     1261 gaggtgcggg acgggaagct ctacgcggtg tcgatagacg acgcctagac gtccgccagg
     1321 gcttccttca gctcctgagg cgacaccgtg ccctcgaact tcgccgccac cttgcccttc
     1381 gcgtcgacca cgaagaccca cggctcgctc gggaggcccc actccttgac cgccggcact
     1441 ggcttcaggt tcgaggggtc gtccaggttc tcgtagacct cgacgtggat gaactcgacc
     1501 tgcggccagt ccttcgactc cttcttgacg atgtcgagcg tcggggcgca cacggccgag
     1561 gtgcagaact tcggcgtcgc gaacgtgacg acgaacggat cgcccttctc cacggcctcg
     1621 tggatcgagg actcgtagaa gtccgggtcg ggtttcgtgt ccgtggtgat cttctcgagc
     1681 ttgcgcccgg acgcggtgag cgtcttggac gccggcgcgg gctcgccgat cgccggcgtc
     1741 gtcccgtcct ttccgacgtc gaagcccgcc cgcacggtct cgtcgaggcc gtcaccggac
     1801 acggtcacct cggcccccca cctgcccgcg gagtcgaacg tcgggtacgt cacgtagagc
     1861 ccgcgggggc cggtgtcgat gaactcggcg tcggtctcga aggccggctc ttcggcggac
     1921 tcggcgaggt cgaagaacgc gacgtgcatc gagatgtccg gcgaggagat gggcgcgtcc
     1981 tcgtcgttga gcaacccgat cagaaaccgg ttctcgccct cgacgacctc cgagctggcg
     2041 aacaccgggt aggccttcac gtcgtcgaac ggctgctcga acgactcggc ccccgagctc
     2101 gcggagtcgg acttgtccga accgccggac ccgccgccgt cgtcgctccc gccgcacgcg
     2161 gacgcggtca gcgcgagcag cgcggctatg gctccgagac acaatctccg cttcatcagt
     2221 tcggcagcct agcgggcggt cgcgtttagg ggcgcagata gaatcgccgc accgatgacc
     2281 ctctttcgca ggctcacgag cgcgtccctg atcgccacga tcgtgttgat cgcgatcggg
     2341 ggcttggtcc gcgccaccaa gtccgggctc ggctgcggca ccgactggcc ggactgcggc
     2401 ggaaggctcg tcccggcgct cgagacgagg gccgagatca tcgagttctc ccaccggttc
     2461 accgccgcga tcgtcgggtt cctgatcctc gccctcgcga tcgtcgccgt gcggcgcctg
     2521 cgcgacgagc gccgcatcaa ggtcgcgtcg gtggcggcgc tggtcctcgt ggtcttccag
     2581 gcgcttctcg gcgcggcggt cgtcaaggcc gagctcgagc gcctcgtcgt cgtcgcccac
     2641 ctcgtgatgg cgatgagcct gctcgcgttg ctcgtctacc tggccgggat ggtggccgcc
     2701 cgcacgcgcg gcctcgacgg cgcgaccgac gcgcggctca gccggcggat cgcgctcgcc
     2761 ggcctcggcg tcctcgcgct gctcgtcgtc gggtcgctcg cgtccacccg ggacatcggt
     2821 ccgtcgtggc ctctcgtcaa cggcgagctc gtgcccgacc tcggcgtcga gacgttcgcc
     2881 ctgcactggt tccaccgcgt cctggccggg atcgtcggga tcgccgtctt cgccgtcgcc
     2941 tggagcgtga tcaagcggcg tgacgagatg ccggccgcgg cccgctacgc ccacgcagcg
     3001 gcgggcgtct tcgggttcga aatcttcgtc ggcgcgctga acctgtggac cgacctcaac
     3061 gccctggtcg tcgccttgca cctcacgacc ggtgccttga tctgggctag ctttgcggcg
     3121 atggtcaccg tcacgcaccc ctccgtccgc gccgcttccg aagcgtccgt cgcccggggg
     3181 gtcccggcga tggcggcggg cggccgctga cgtgaagagg ctggtgatcg gcgagccgac
     3241 gggcacgctg accgtcgagg aggtcgtcca cgcgccggtc gccgaggtcg aggtcacggc
     3301 tccggcaacc tttcggcagc gggcagccgc ttacttcgcg ctgacgaagc cgcggatcgt
     3361 cgagctgctg ctcgtgacga cggtgccgtc gatggtcgtc gcggcgcagg gctggccctc
     3421 gtggctgctc gtgctgaaca cgctgatcgg cggcgcgctg tcggccggtg gggcgaacgc
     3481 gatcaactgc tacctcgatc gcgacataga ccgcgtgatg ccgcgcacga agcggcggcc
     3541 gctgcccatg caccaggtcg agccggcgcg cgcccttgcg ttcggcgtga tcctcggcgt
     3601 cgtcgcgttc gcgtacctgt gggcgacggt gaacctcatc tcggccgtcc tcaccacggt
     3661 cgcgctgctc ttctacgtct tcgtctacac gatcggcatg aagcgctcga cgccgcagaa
     3721 catcgtgatc ggcggcgcgg ccggcgcgtt ccccgcgctc gtcgggtgga gcgcggtgac
     3781 cgggaccgtc gagctgcccg cgctggcgct gttcgggatc atcttctact ggacgccgcc
     3841 gcatttctgg gccctcgcga tgcgatacga gaaggactac gccgcagcgg gcgtgccgat
     3901 gatgccggtc gtgtacggcc gcgaggagac cgcgaagcac gtcctgctct attcgttcct
     3961 gctgcttgcg atgtcgctcg tgttcttctc cgtcggcagg atgggcgcga tctacctggt
     4021 ctcggccctc gtgctgaacg gcgtgttcct ctacggcgcg atccgcctct accgcaacac
     4081 ggcgccgacc gtcgcgtggt ggctgttccg gttctcgatc tactacctgg cgctgctctt
     4141 cggcgcgatg gcgctcgacc gcttgctgct ggcctgaccg gcccggtctg ggcaggggtc
     4201 gggggcaagg acgcgtacag cgcggagcag gagaagggtt gcgagcgccg acccggcggt
     4261 gcagaaccgg gcgaacgggg taacacggac ctgaccgaac caagccgtcc cgccctggag
     4321 gtacccgatg tctcgccggc tcttcgcgct ctcgttgctc ggcgccctgc tactgacctc
     4381 gctccccgtc tcgatcgcgg cgcaggagca gggccccccg aaggtccccg acaccatgga
     4441 ggcctactcg atcgtcccgc ccggccagga cggcaacgtc acgccggacg aggttccctc
     4501 gggtgacttc ggcccccact tcgacgacca gcgcgagctg tacgcctcgc tgatcgacga
     4561 cgaggacgtc accgacgacg agatcgcgac ctacttccac tcgatgcagt tcggcccgaa
     4621 agaggtcgag cgcgactacc agccggtcga gggcgtcacg gtctaccgcg acgagttcgg
     4681 catcccgcac atctacgcgg actcgttcga caccgcgagc cacgcgctcg gctacacgac
     4741 ggccgaggac cgcctgtggg agatggacgt cttccgccac gcggcgaaag gcgagctgtc
     4801 gacgctgatc ggccccgact acctccccat ggacgtcacc gcgcgccgcg agggatacac
     4861 cgaggaagag gtccagaaga tgtacgacga cctcgacgac aagttcggcg cgaccggcaa
     4921 gacggtgcag gacgggctgc aggcctacgc cgacggcgtc aaccagtaca tcgcggagct
     4981 gcggacgaac ccggcacagt gccccgccga gtaccaggcg ctcggcaacc cgtgccccga
     5041 gcccgagccc gcggactgga cgccgctcga cacgctctac gtcgcgatcc tccagctgcg
     5101 cgtgttcggc gagaccgccg ggaccgagct cgacaacgca ggcctgtacg cgcacctcgt
     5161 cgagaagcac ggctcgaagc tcggaccgaa gatctacgag gacttcgtga gccgcaacga
     5221 ccgccgttcg gcgacgtcga tcccggcacg tgagggccgc ttcccgtcgc aggccctcgg
     5281 caagaccaag aaggcttcgt tcgcgatacc ggacgacgcg ccggcgctcg cccgccagac
     5341 gtcgagagag cagaagcgcg tccaggactt cctcgcatcg atgggcttcc gtgcccccgc
     5401 ttcgaacgcc ttgatcgtcg gcgcgaagga gtcggcatcg gggaacccga tgcagatcgg
     5461 cgcgccgcag gtcggctacg cggtcccgtc gttcttctgg gacgtcgacg tgcacgtgtc
     5521 gggggaggac gaggcgcact tccgcggccc ggcggtcccc ggcgcgtccg ctctcgtgcc
     5581 gctgggacgg gggccggact acgcgtggac tctgaccacc ggatactccg acgccgtcga
     5641 cacgaaggtc gagctcctct gcgaccccgc cggcggcgag gcggcgcagg actcgaacgg
     5701 atacatgttc gagggcgagt gcagggagat ggagtcgcgc tcggagacgt tcgtcgtgaa
     5761 gccgtccgcg ggatcgcccg gaccgcccag cgtcgagcag cgcacgttct accggacggt
     5821 ccacgggccg gtgttcgcgc gcggtgaggt cggcgggaag cccgtcgcgt tcgtcaagca
     5881 gcggttcttc tggaagcgcg aggtcgactc gatcccgcag ttctacaagt ggaacaccca
     5941 ggtcgactcg atcgaggact tcgcgaaggc ggccggcaag ttcacgatgt cgttcaacac
     6001 cttctacgcc gacgaccgcg acatcggcta cttccacgtc ggctactacc cggtgcgtcc
     6061 gaaggggtac cacccgagcc tgccgacgtg gggcaccggc gactgggagt ggaagggacg
     6121 gctgccgtac tcgcgtcacc cgaaggtgat caacccgaag cagggatgga tcgcgaactg
     6181 gaacaacaag cccgcgaccc gctgggacaa ctacgacggc atcaagtggg gctcgatcca
     6241 ccgcgtcgag ctcttgcaga acgagatgca caggcacttc gacggcaagc gcaaggggag
     6301 gctgtcggac ctcgtcgacg tcatccgcca ggcagcgacg cgcgacgccc gcgcggtcta
     6361 cctcggacgg aagatgacgc gctggggcgg ggcagccgag gccgcggaag ctgaccaggc
     6421 gctgaagctc gttcgcgcgt gggtcaaggg cggcgcgcac cggcacaacc gcgactggaa
     6481 ggacggcgac acggcggagg acaacggtcc ggccgccgcg atcttcgacg cgtggttcga
     6541 cgcgctcgtc caccgggtgt tcgacgacga gatcggcgcg gacgcgtacg acctggtagg
     6601 caccccggtc accgaccggg acatgtggca cgacttctcg tcgtacctcg acaacctgtt
     6661 ctcgaagagg gccgcgcgcg gctacgcccg caactactgc gacaacctcg acacgccggc
     6721 caaggagggc tgcagggccc aggtggccgc ggccctcgac gacgcgctcg cggcgctgaa
     6781 ggaggcgcag ggcgaggacg tgtcggcgtg gacgacggac gcctggatga ccgagttcga
     6841 gagcctcggc ctcggctcgg cggacccgat cccgtggcag aaccgcggta cgcacaacca
     6901 cgtagtcgaa atcttgcgta aagcacgtta accctcttgt tcactccgcc accggtggac
     6961 gaggctgtac gccgtcggta ggtgccg
//
LOCUS       DASQDO010000516         6655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_422109, whole genome shotgun sequence.
ACCESSION   DASQDO010000516 DASQDO010000000
VERSION     DASQDO010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422109"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /gene="gnd"
                     /locus_tag="VEV43_14110"
     CDS             <1..744
                     /gene="gnd"
                     /locus_tag="VEV43_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYP24700.1"
                     /translation="LVEKLTPPKAVWAMLPAGEVTGSTIARLADLLDAGDVVVDGGNS
                     RHVDSVARASLLAEAGIEFVDCGTSGGIWGLTEGYCLMVGASEETFARLEPIFATLAP
                     EDGYARVGPPGAGHFTKMVHNGIEYALMQAYGEGFQLMHEGGFDVDVARVAELWRHGS
                     VVRSWLLDLAARALSGDPELASLDDYVEDSGEGRWTLEYAIEHAIPAPVIALSLFERF
                     SSRQESSFSNKLVAALRKEFGGHGVRPAE"
     gene            741..1031
                     /gene="clpS"
                     /locus_tag="VEV43_14115"
     CDS             741..1031
                     /gene="clpS"
                     /locus_tag="VEV43_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HYP24701.1"
                     /translation="MSIAPDTIEEITNDEATDLERPWLVIVWNDPINLMSYVTWVFQK
                     LFGYSFEKATQLMLDVHHKGKAVVSSGPREKAELDCYRLHAHGLWARLTKSD"
     gene            1033..1524
                     /locus_tag="VEV43_14120"
     CDS             1033..1524
                     /locus_tag="VEV43_14120"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HYP24702.1"
                     /translation="MRVGRGPVLHLEEHEAGLLRQLVDETRTLLEAGVPRADPVIARL
                     FPDAYDDPEDAQSYHDMVGDELRKEKVRSLAAVSESMGPAGRADVELNDELVTARLAW
                     LTDVRLAVGTRLGVTEEKMGAEIDPASPEAPAYEVLHWLGWIQESIIERIGPLGGGRS
                     EED"
     gene            1529..1984
                     /locus_tag="VEV43_14125"
     CDS             1529..1984
                     /locus_tag="VEV43_14125"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYP24703.1"
                     /translation="MTQLERGDKAPDFSLEDADGTTWSLADLRGQKVIVYFYPADDTP
                     GCTAQACDFRDAQPRIDEAGFRVFGVSPQGASSHRRFSDKHSLNFPLLIDSDYTMARE
                     YGVAEEAEGKKPRIKRSTFVIDEDGTLLEAQYGVRAKGHVESLLSGISG"
     gene            complement(1981..2730)
                     /locus_tag="VEV43_14130"
     CDS             complement(1981..2730)
                     /locus_tag="VEV43_14130"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HYP24704.1"
                     /translation="MIIGAHVPGDAPLEEAAAVEAECVQMFVGPPQSWKKPPSRPDAA
                     ELVASPLPVYVHAPYLINVASPNNKVRIPSRKILTDTLDRAAEVGAAGVIVHAGHAED
                     GIERGFVRWVKCFEEVEAPVPILIENTAGGTNAMGRHVDVLAELFDHLKGFDAGFCFD
                     TCHAHAAGEDLADVVERVRAATGRIDVLHANSSRDEAGTGADRHANFTDGKLAPEAIL
                     EMIQASGAPAVICETPWPAIADDIAWLRERL"
     gene            2793..3194
                     /locus_tag="VEV43_14135"
     CDS             2793..3194
                     /locus_tag="VEV43_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYP24705.1"
                     /translation="MKLLRDVAIRHLVTVGPATTLRRAAKVMTDRGVGCAVVVENERV
                     AGIVTERDVLRAIAEDGKMDEVTVADVMTRDVVSGSPGWELLKAVHTMTEGGFRHLLV
                     MEMDDPVGIVSLRDLMTAMADLVAADGTPSA"
     gene            complement(3191..3631)
                     /locus_tag="VEV43_14140"
     CDS             complement(3191..3631)
                     /locus_tag="VEV43_14140"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYP24706.1"
                     /translation="MARKLTIEVSRVARCEPKTVMELVRDPKTWPRWQPEIVSTEGPA
                     PLNEGDDVYGHAEMLGFDVEGHSRTVSVTPDAYEEDVVVGVRMRIVYEVSRDDAGNTV
                     VTRRLSAQLPGGLAGRLLSFFLKRRLTRMQDGVIEDLVRQAERR"
     gene            3664..5154
                     /locus_tag="VEV43_14145"
     CDS             3664..5154
                     /locus_tag="VEV43_14145"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYP24707.1"
                     /translation="MAKPRLGIAAGNKSGTESAAEVARQGGNAVDACLAAAITGWVAE
                     PFFTSLAGSGFVAVRTPEGKVEIIDGNNAMPTTEPAEPNQGVRRVFLEYSNGMYTGIG
                     GGAVAVPGVLAAVRVAWERHGKIEWPALFQGAIEVAREGLPFPKTSAYYLSVTWNEIW
                     SQFEGSRELFVVDGRPMREGETLCQPDLADALERIAEEGPDVFYCGDVATDIGDAIAR
                     DGGFLGVSDLKAYRAEVRSPIGTDAGGWRIESNPPPAVGGAVLIHMLALLGDSALDEP
                     VERARALVEAQRNAIGYRAEKYQDPDLIAAAFDEAVKGFTTRRSSDTTHASSADADGY
                     ACSITESNGYGAGLCVDGILLNNTLGEEELNPFGAHGLRPGTRCHSNMTPTIARGGGR
                     TVAIGSPGADRIVTAIAQTLVRLAFDGASLKEAVAGPRIHLDPRPSGELLCFEPGIPG
                     HQVGYNPLPYDDIHMYFGAVQVASVTDDGDVDAAFDPRRSGGAALI"
     gene            5195..5761
                     /locus_tag="VEV43_14150"
     CDS             5195..5761
                     /locus_tag="VEV43_14150"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HYP24708.1"
                     /translation="MARLFSIGPGISMKGRKFKGLRGFAGKPFHPPLTDLVVGAYFFV
                     GIFDLISYVAKDPQTEYDFFRAGTILLISGALFSLPTMLTGFWDWLKSTPKHTQVWRT
                     ANFHMAAMLTTGVLVLIDILMRSGQEEPRAGLGLAALSLAITGLMTLGATYGGSLTYD
                     YSFNVEELDGRVWEKSETDIYPKDKPLK"
     gene            6015..>6655
                     /locus_tag="VEV43_14155"
     CDS             6015..>6655
                     /locus_tag="VEV43_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24709.1"
                     /translation="MPKSIPPRTTGAVCALLVLSLMGSAAATPAGSGREIPPVLLGLI
                     ETATDKGEIRLLVSVRDMAAQRTVLDTIDKSDARVLHRYEILPYLLLEAGPDTVRSLV
                     HDPLVTGFTEDLPEKATLDSSIPVVNGDDVRALGLRGGGTAVAILDSGIDLDHPFLGN
                     VVSEACYSDFTGTSSGTSLCDGGAHSDVGPGAADVESGGDEPCISSGTNICSHG"
BASE COUNT         1040 a         2239 c         2360 g         1016 t
ORIGIN      
        1 ctcgtcgaga agctcacgcc gccgaaggcc gtgtgggcga tgctcccggc cggcgaggtc
       61 acgggctcga cgatcgcgcg gctcgcggac ctgctcgacg ccggagacgt cgtcgtcgac
      121 ggcgggaact cacgtcacgt cgactccgtc gcgcgcgcct cgctcctcgc agaagccggg
      181 atcgagttcg tcgactgcgg gacctcgggc ggcatctggg gcctgaccga gggctactgc
      241 ctgatggtcg gagcgtccga ggagacgttc gcgcggctcg agccgatctt cgcgacgctc
      301 gcgccggaag acggctacgc gcgcgtcggc ccccccggcg ccggccactt caccaagatg
      361 gtccacaacg ggatcgagta cgcgttgatg caggcgtacg gcgaaggctt ccagctcatg
      421 cacgagggcg gcttcgacgt cgacgtcgca cgggtggccg agctgtggcg ccacggcagc
      481 gtcgtgcggt cgtggctcct cgacctcgcg gcgcgcgctc tgagcggcga tcccgagctg
      541 gcgtcgctcg acgactacgt cgaggactcg ggcgagggca ggtggacgct cgagtacgcg
      601 atcgagcacg cgatcccggc ccccgtgatc gcgctgtcgt tgttcgagcg gttctcgtcg
      661 cgccaggaga gctctttctc gaacaagctc gtcgccgcgc tgcgcaagga gttcggcggg
      721 cacggcgtca ggcccgcgga gtgagcatcg cccccgacac catcgaagag atcacgaatg
      781 acgaagcgac cgacctcgag cggccctggc tcgtcatcgt gtggaacgac ccgatcaacc
      841 tcatgtcgta cgtgacgtgg gtcttccaga agctcttcgg ctactcgttc gagaaggcga
      901 cccagctgat gctcgacgtg caccacaagg gcaaggcggt cgtgtcgagc gggccgcgcg
      961 agaaggccga gctcgactgc taccggctgc acgcgcacgg gctgtgggcg aggttgacga
     1021 agagcgactg aggtgcgagt cggtcgcggg cccgtcctgc acctcgaaga gcacgaggcc
     1081 ggtctgctgc gtcagctcgt ggacgagacg cgcacgctgc tggaagccgg cgtcccgcgg
     1141 gccgatcccg tgatcgcccg tctgttcccc gacgcctacg acgaccccga ggacgcgcag
     1201 agctaccacg acatggtcgg cgacgagctg cgcaaggaga aggtgcgctc gctcgccgcg
     1261 gtgtcggagt cgatgggccc ggcaggacgc gccgacgtag agctgaacga cgagctcgtg
     1321 acggcgaggc tcgcatggct caccgacgtg aggctcgcgg tcgggacgag gttgggcgtg
     1381 accgaggaga agatgggggc cgagatcgac ccggcgtcgc cggaggcacc ggcctacgag
     1441 gtgctccact ggctcgggtg gatacaggaa tcgatcatcg aacggatcgg ccccctcggc
     1501 ggcggccggt cggaggagga ctgatcacgt gacgcagctg gaacgcgggg acaaggcccc
     1561 cgacttctct ctcgaggacg cggacgggac gacgtggagc ctggccgatc tccggggtca
     1621 gaaggtgatc gtgtacttct atccggccga cgacacgccg ggctgtaccg ctcaagcgtg
     1681 tgacttccgc gacgcgcagc cgcgtatcga cgaggccggg ttccgggtgt tcggcgtcag
     1741 cccgcaggga gcgtcgtcgc accgcaggtt ctccgacaag cactcgctga acttcccgct
     1801 gctgatcgac tcggactaca cgatggcgcg cgagtacggc gtagccgagg aggccgaggg
     1861 caagaagccg cgcatcaagc gttcgacgtt cgtgatcgac gaggacggga cgctgctgga
     1921 ggcgcagtac ggcgtgcgcg ccaagggcca cgtcgagtcg ttgctgtcgg ggatctcggg
     1981 ctagagccgc tcccgcagcc acgcgatgtc gtctgcgatc gccggccacg gcgtctcgca
     2041 gatcacggcc ggggcccccg aggcctggat catctcgagg atcgcctccg gcgcgagctt
     2101 gccgtcggtg aagttcgcgt gacggtctgc ccccgtcccg gcctcgtcgc gcgagctgtt
     2161 cgcgtgcagg acgtcgatcc gtccggtcgc ggcgcggacc ctctcgacga cgtcggccag
     2221 gtcctcgccc gccgcgtgcg cgtgacacgt gtcgaagcag aagcccgcgt cgaatccctt
     2281 cagatggtcg aacagctcgg cgaggacgtc gacgtgacgg cccatcgcgt tcgtcccgcc
     2341 cgcggtgttc tcgatgagga tcgggacggg cgcctcgacc tcttcgaagc acttgaccca
     2401 gcggacgaac ccgcgctcga tcccgtcctc cgcgtggccg gcatggacga tcacgcccgc
     2461 ggccccgacc tcggcggccc ggtcgagcgt gtcggtcagg atcttgcgcg acgggatacg
     2521 gaccttgttg ttcggcgacg cgacgttgat gaggtagggg gcgtggacgt agaccgggag
     2581 cggcgacgcg acgagctccg cggcgtcggg gcgcgacggg ggcttcttcc acgactgagg
     2641 gggcccgacg aacatctgga cgcactcggc ctcgacggcc gcggcctcct cgaggggggc
     2701 gtctccgggg acgtgcgctc cgatgatcac gcccagaagc tagccgaagt ctccggttgc
     2761 ccggcccaac gatctggcag atgatgtcgc ccatgaagct cctgcgcgac gtcgcgatca
     2821 ggcatctcgt caccgtcggc ccggccacga cgctgcggcg ggccgcgaag gtcatgacgg
     2881 accggggcgt gggctgcgcc gtcgtcgtgg agaacgagcg cgtcgccggc atcgtgaccg
     2941 agcgcgacgt cctgcgggcg atcgccgagg acgggaagat ggacgaggtc acggtcgccg
     3001 acgtcatgac gcgcgacgtg gtgagcggtt cgccgggctg ggagctgctg aaggccgtcc
     3061 acacgatgac cgagggcggc ttccgccacc tgctcgtgat ggagatggac gacccggttg
     3121 ggatcgtctc tttgcgcgac ctgatgacgg cgatggccga tctggtggcc gcggacggca
     3181 ccccctcggc ctagcgccgc tccgcctgcc gcaccagatc ctcgatcact ccgtcctgca
     3241 tgcgcgtcag cctgcgcttc aggaagaacg acagaagcct gccggcgagc ccgccgggga
     3301 gctgcgccga gagccggcgc gtcacgaccg tgttcccggc gtcgtcgcgg ctcacctcgt
     3361 agacgatgcg catcctgacg ccgacgacca cgtcctcttc gtacgcgtcg ggggtcaccg
     3421 agaccgtcct gctgtgcccc tcgacgtcga agccgagcat ctccgcgtgc ccgtagacgt
     3481 cgtctccttc gttcaggggg gccgggccct cggtcgagac gatctcgggc tgccagcgcg
     3541 gccacgtctt cgggtcgcgc accaactcca tcacggtctt cggctcgcag cgcgcgacgc
     3601 gcgatacctc gatcgtgagc ttgcgagcca tcgcgcgagt ctaggagggg ctaacctccc
     3661 ggcatggcga agcctcgtct gggcattgcg gcgggcaaca agtcggggac tgagtccgcg
     3721 gccgaggtcg cgcgccaggg cggcaacgcc gtcgacgcct gtctcgccgc cgcgataacg
     3781 ggctgggtcg ccgagccctt cttcacctcg ctcgccgggt ccggcttcgt ggcggtgagg
     3841 acgcccgagg ggaaggtcga gatcatcgac ggcaacaacg cgatgccgac caccgagccg
     3901 gcggagccga accagggcgt gcggcgggtc ttcctcgagt actcgaacgg gatgtacacg
     3961 gggatcggag gaggggcggt cgcggtgccg ggcgttctag ccgcggtgcg cgtcgcgtgg
     4021 gagcgccacg ggaagatcga gtggcccgcg ctgttccagg gcgcgatcga ggtcgcccgg
     4081 gagggcctcc cgttccccaa gacgtcggcg tactacctct cggtcacgtg gaacgagatc
     4141 tggtcccagt tcgagggctc gcgcgagctg ttcgtcgtcg acggccgtcc gatgcgcgag
     4201 ggcgagacgc tgtgtcagcc cgatctcgcc gacgcgctcg agcgcatcgc cgaggagggc
     4261 ccggacgtct tctactgcgg tgacgtcgcg accgacatcg gcgacgcgat cgcgcgcgac
     4321 ggcggcttcc tcggcgtgtc ggacctcaag gcgtaccgcg ccgaggtgcg gtcgcccatc
     4381 ggcaccgacg cggggggatg gcggatcgag tcgaacccgc ctcccgcggt cgggggggcc
     4441 gtgctgatcc acatgctggc gctgctcggc gactcggcgc tcgacgaacc cgtcgagcgc
     4501 gcgcgggctc tcgtcgaggc gcagcgaaac gccatcggct acagggccga gaagtaccag
     4561 gaccccgatc tcatcgcggc agcgttcgac gaggccgtga agggtttcac cacgcggcgg
     4621 tcgtcggaca caactcatgc gtcgagcgct gacgcagacg ggtacgcgtg ctcgatcacc
     4681 gagtcgaacg gctacggcgc cggcctctgc gtcgacggga tcctgctgaa caacacgctc
     4741 ggcgaggagg agctgaatcc cttcggagcg cacggcctcc gtcccgggac gcgctgtcac
     4801 tcgaacatga cgccgacgat cgcgcggggt gggggccgga cggttgcgat cgggtctccg
     4861 ggcgcggacc ggatcgtcac cgcgatcgca cagacgctgg tgcgcctcgc gttcgacggc
     4921 gcgtcgctca aggaggccgt cgcagggcct cgcatccacc tcgacccccg gccctccggc
     4981 gagctgctgt gcttcgagcc cggcatcccg ggccaccagg tcggctacaa cccgctcccc
     5041 tacgacgaca tccacatgta cttcggggcc gtccaggtgg cgtcggtgac cgacgacggc
     5101 gacgtcgacg cggccttcga cccccgccgc tcgggaggcg ccgcgctcat ctaagctcgg
     5161 tccgtcgtcc cgcagtttcc gaaaggggat cacgatggcc cgtctgttct cgatcggccc
     5221 cggcatctcg atgaaggggc ggaagttcaa gggcctgcgc ggcttcgcgg gaaagccgtt
     5281 ccacccgccg ctgacggacc tggtcgtcgg tgcctatttc ttcgtgggga tcttcgacct
     5341 gatctcgtac gtcgcgaagg atccgcagac ggagtacgac ttcttcaggg ccgggacgat
     5401 cctgctgatc tcgggggcac tgttctcgct gccgacgatg ctgacgggct tctgggattg
     5461 gctgaagtcg acgccgaagc acacccaggt ctggcgcacg gcgaacttcc acatggcggc
     5521 catgctgacg acgggcgtat tggtgctgat cgacatcctg atgcggagcg ggcaagagga
     5581 gccgagggcc ggcctgggcc tggcggcgtt gtcgctggcg ataacggggc tgatgacgct
     5641 aggagcgacc tacggcggga gcctgaccta cgactacagc ttcaacgtag aggagctgga
     5701 cggccgggtc tgggagaagt cggagacgga catctacccg aaagacaagc cgctgaagta
     5761 gggggctagg ggggccgagg tcgtggccgc cctgaaccgt agcgatgctt cccggtaccg
     5821 cggcctcggc cagccgcgag ccatccctgc tcctattccg tcccgcccgc gcggatgtag
     5881 cgcagctaac agcgcaacgg cccctcgtgc gcgagaaagg aacagaccca cccggggacc
     5941 gtcccgcacg gacagcaagc atctgctctc ttctgcggct ccgacgcttc gggccatccc
     6001 gaaaggagag cagcatgcct aagtccatac ctccccgcac gaccggagcg gtgtgtgcct
     6061 tgttggtcct ctcgctcatg ggatccgcgg ccgcgacgcc cgccggatcc gggcgcgaga
     6121 tcccacccgt cctcctgggg ttgatcgaga ccgcgacgga caagggtgag atccggctgc
     6181 tcgtctccgt gcgggacatg gccgcacagc ggacggttct ggacaccatc gacaagtccg
     6241 acgcgcgagt cctccaccgc tacgagatcc tcccctacct cctcctcgag gccgggcccg
     6301 acaccgtccg gagcctcgtg cacgacccac tcgtcacggg gttcaccgag gacttgccgg
     6361 aaaaggcgac gctcgacagc tcgatccccg tcgtcaacgg ggacgacgtg cgggccctcg
     6421 ggctccgcgg cggcggtacg gcggtggcga tcctggacag tgggatcgac ctcgaccacc
     6481 cattcttggg caacgtcgtg tccgaggcct gctactcgga cttcacgggg acgagctcag
     6541 gaacgagtct ctgtgacggc ggagcgcatt cggacgtcgg ccccggtgcg gccgacgtcg
     6601 agagtggcgg ggacgaaccc tgcatcagca gcggcacgaa catctgcagt cacgg
//
LOCUS       DASQDO010000517         7107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_543261, whole genome shotgun sequence.
ACCESSION   DASQDO010000517 DASQDO010000000
VERSION     DASQDO010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543261"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VEV43_14160"
     CDS             complement(<1..538)
                     /locus_tag="VEV43_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP24710.1"
                     /translation="MSDSVAFDRAVDYYDRTRALPPGVEAAMVEAVSAELAGRGRVLE
                     IGAGTGRIALPLRAAGVDVVGLDLSLPMLERFVAKAGGAAPVVGGDATRLPFRDRSFG
                     AAFAVHVLHLIPSWKVAVRELLRVVEPGGVLLVSQGWWGLLAYLDVIEAYTEAAGIEL
                     RHVGLNDEAELDAALPDAT"
     gene            607..810
                     /locus_tag="VEV43_14165"
     CDS             607..810
                     /locus_tag="VEV43_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24711.1"
                     /translation="MKKIVAGVALAAALLVPATAAQAASEPGPPAGVRPCGPMQIGVV
                     VWVANPKTGETEDVAEYCEPIGP"
     gene            849..1049
                     /locus_tag="VEV43_14170"
     CDS             849..1049
                     /locus_tag="VEV43_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24712.1"
                     /translation="MRKLVASACLAGALLLPVAPASADERPGAGVEDCRDYGGGYYGV
                     WAWYYDLENDYHRIWTCIYLGP"
     gene            1071..1385
                     /locus_tag="VEV43_14175"
     CDS             1071..1385
                     /locus_tag="VEV43_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24713.1"
                     /translation="MKKLIAAAFVAATVVAPVAPAHADDTGPIVRTEECPPGRVGRTI
                     HIGDNISVYNCYPVPGRTSEGAAIVRLADCPPGYTGYVVQAWNEKRGWYDLVVFCIPF
                     GP"
     gene            1608..1754
                     /locus_tag="VEV43_14180"
     CDS             1608..1754
                     /locus_tag="VEV43_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24714.1"
                     /translation="MATTEDGAARAGVGNCQDRQAGYRGVYVWWYDLENNYNERKACI
                     YVGP"
     gene            1835..2038
                     /locus_tag="VEV43_14185"
     CDS             1835..2038
                     /locus_tag="VEV43_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24715.1"
                     /translation="MKKLILTAVAAAALALPVAPAAASPGTAVDDSGPARAGVTFCGT
                     TGHYIIWYYDANNQYRELWNSCI"
     gene            2112..3410
                     /gene="purB"
                     /locus_tag="VEV43_14190"
     CDS             2112..3410
                     /gene="purB"
                     /locus_tag="VEV43_14190"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYP24716.1"
                     /translation="MIPRYSREEMAAIFSERAKFSRWLDVELLAVEARVRLGEVPVED
                     LTEIKEKAAFDVARIEEIEAVRHHDVVAFVENVRENVGEAGRHIHYGMTSSDVLDTAN
                     AVALRDAGDLLVEETGRLTETVRRKALEHRDTLMAGRTHGVHAEPTTFGLKLAGWTFE
                     LARDRDRLRRARDEVAVGKLSGAVGSYSQLPPDVEAYVCDRLGLKVEDAATQVIPRDR
                     HADFLGAIALTGASLERFAQEIRHLQRTEVREVEEPFAQGQKGSSAMPHKRNPIVCER
                     ICGQARLLRAYAMTGFENVALWHERDISHSSVERVTFGDACVLLDYMLAKMRWVVDGM
                     VVHADRMRRNLEASWGLVHSQGVLTALLGKGTLQREEAYALVQRNSMTAWDEGRPLKE
                     LLKSDPQVTEHLDPDEIEACFDPSGYLEKAEVVWERLEEL"
     gene            3431..4336
                     /locus_tag="VEV43_14195"
     CDS             3431..4336
                     /locus_tag="VEV43_14195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYP24717.1"
                     /translation="MGSGKVREIYRVDDETLLLVATDRISAYDVVLPQQIPDKGKVLT
                     GLTHHWLTTMSSICPNHLISVAEKDLPDVGITNDRLGDRPWADDLAGRAMLCKAAEPL
                     PIEFVVRGYLSGSGWREYVEQGSVCGIPLPEGMKESDRLPRPLLTPATKALEGHDENV
                     TEERAAEIAGKDRYDAAKEYALALYATAAEQALERGVIIADTKFEFGVHDDEVILIDE
                     VLTPDSSRFWPQDQYAPGGPQPSFDKQYVRDWLDSTGWDHSPPPPDLPADVVEATRSR
                     YVEAYEKVTGNAWDHWDAVVTGGAA"
     gene            4333..5013
                     /locus_tag="VEV43_14200"
     CDS             4333..5013
                     /locus_tag="VEV43_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HYP24718.1"
                     /translation="MSSAVSSSQGPARLGATVRGVLVSPGSAFVPALRRVETSQEGTS
                     TYVLAALGGAAAMLLWLKVRALVGFRSLAPSDFSWSYFVISLVAGAIVAVLAQVVWSF
                     VGPAAGGPDSGSPRSFRAVWALSAFPQVPALLVLAPIDLLLVGTQAFTASDTGDSVAL
                     AWLALSTAGAVSLAAWSAYVFAKGTHVATRAAPRRTAGVVAAGVLCLGGVAALLFGAL
                     AGLGEVLS"
     gene            5010..5255
                     /gene="purS"
                     /locus_tag="VEV43_14205"
     CDS             5010..5255
                     /gene="purS"
                     /locus_tag="VEV43_14205"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012307160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HYP24719.1"
                     /translation="MTFTAHVNVMLKPGIADPQGQTIEKALPVLGYEGVGDVRVGKRI
                     ELRLDAADESDARDKVDAMCKQLLSNPVIESYEVDLT"
     gene            5252..5944
                     /gene="purQ"
                     /locus_tag="VEV43_14210"
     CDS             5252..5944
                     /gene="purQ"
                     /locus_tag="VEV43_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HYP24720.1"
                     /translation="MTATVGVVVFPGSNCDLDAQRAFADLGADVRMVWHEETSLDGID
                     LVVLPGGFAHGDYLRTGALARFSPVMDAVIEHAERGGLVAGICNGFQILCEAGMLPGA
                     LRKNAGLKFLCKWVDLRVENASTPFTARAEPGQILRIPINHFEGNWYCDADQLEELKA
                     NDRVVLRYADNPNGSLDDVAGIVNERGNVFGLMPHPERACESLLGSEDGRLILGSMLD
                     HVAAQHELSVAG"
     gene            5947..>7107
                     /gene="purL"
                     /locus_tag="VEV43_14215"
     CDS             5947..>7107
                     /gene="purL"
                     /locus_tag="VEV43_14215"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HYP24721.1"
                     /translation="MALHRDLGLTDEELEAIRDRLGRDPSYTELAMFSVMWSEHCSYK
                     SSRVHLKTMPTEGAHVVVGPGEGAGIVEVAPGVRIAWKVESHNHPSFVEPFNGAATGV
                     GGIVRDILSMGARPIALMDSLRFGPLDDARTRYLVDGVVHGISSYGNSIGVPTVGGET
                     VFEDCYAENPLVNVACLGIVEDALMHGRAEGPGNPVVLFGSSTGRDGIGGASILASAE
                     FDEDSLEKRPSVQVGDPFTEKLLIEASLELIRRGLVAGFQDLGAGGISCPTSEMTAKA
                     GTGMVVDVDKVHRREPGMEPFEVMISESQERMLAVVEPKDLDDVLEVCRRWGLEASVL
                     GEVTKSGRLEVIAGGEVVADAPAEALAEEGPVYQRPLERPAWIDDVQAAPAGD"
BASE COUNT         1095 a         2496 c         2479 g         1037 t
ORIGIN      
        1 gcgtcgcgtc gggaagtgcc gcgtccagct cggcctcgtc gttcagcccc acgtggcgca
       61 gctcgatccc cgccgcttcg gtgtacgcct cgatcacgtc gaggtacgcg aggagccccc
      121 accagccctg gctcacgagt aggacaccgc cgggctcgac cacgcgcagg agctcgcgca
      181 ccgcgacctt ccacgagggg atcaggtgca ggacgtggac ggcgaacgcc gccccgaacg
      241 aacggtcgcg gaaagggagc cgcgtcgcgt cgcccccaac gaccggagcc gccccgccgg
      301 ccttggccac gaacctctcc agcatcggca gcgacaggtc gagcccgact acgtcgaccc
      361 ccgcggccct cagcggcaat gcgatccggc ccgtcccggc tccgatctcg agcacccttc
      421 cccgcccggc cagctcggcc gacaccgcct cgaccatggc ggcctccact ccgggcggca
      481 gcgcgcgcgt ccggtcgtag tagtcgaccg ccctgtcgaa cgccaccgag tcgctcatcg
      541 ggaggatttt gcccggtggt gtacaagttg tccggattgg gtgagttacc ggaagggggg
      601 aatcgagtga agaagatcgt tgccggcgtg gccctggccg ccgcgctgct ggtccccgca
      661 acggccgccc aggccgcgtc ggagcccggg cctcccgccg gcgtccgtcc ctgcggcccc
      721 atgcagatcg gggtcgtcgt ctgggtcgcg aacccgaaga ccggcgagac cgaggacgtc
      781 gccgagtact gcgaaccgat cgggccctga cccgaaaccg acagccgcat ccaatgaagg
      841 aggacggaat gagaaagctc gtagcgagcg catgcctggc gggggccctc ctgctcccgg
      901 tcgcgccggc ttcggccgac gaacgtcccg gcgcgggcgt cgaggactgc cgcgactacg
      961 gcggcggcta ctacggcgtg tgggcttggt actacgacct cgagaacgac taccaccgca
     1021 tctggacctg catctacctc ggcccgtaga cgaagcgaaa ggtggcgatc ttgaagaagc
     1081 tgatagctgc ggcgttcgtc gcggcgacgg tcgtggcgcc ggtggcaccc gcgcacgcgg
     1141 acgacaccgg cccgatcgtg aggaccgagg agtgcccgcc gggccgggtc ggccggacga
     1201 tccacatcgg cgacaacatc tccgtctaca actgctaccc ggtccccggc aggacgtccg
     1261 agggggccgc gatcgtgcgc ctcgcggact gcccgccggg ctacacgggc tacgtcgtgc
     1321 aggcgtggaa cgagaagcgc ggttggtacg acctcgtcgt gttctgcatc ccgttcggcc
     1381 cgtgagggga gatcgatcca tgaagaagct gatcgcgagc gccgtcgtcg ccgcggcaat
     1441 cgcgttgccc gcagtcccac gcagcccccg cggccgcgtc cgagataccg cctccggccg
     1501 gcaccaagcc ttgcccggcc ccctacacgg gcgggtacat cgtctgggtc ctgataccgg
     1561 gcgtcggtta ccacgaggag tgggcctgca tcccgggcgg tccgggcgtg gcgactacgg
     1621 aagacggcgc agcccgtgcc ggcgtgggca actgccagga tcggcaggcc gggtaccggg
     1681 gcgtctacgt gtggtggtac gacctcgaga acaactacaa cgagaggaag gcctgcatct
     1741 acgtagggcc gtaacggcac ctgcgacgaa ggccgccggg cccgccccgt aagtccgtcc
     1801 gacgtccgtc catcgaaaga gagaggaatc gaacatgaag aagctgatcc tgaccgcggt
     1861 cgccgccgcc gctctggccc ttccggtcgc ccctgcggcc gcgtctccgg ggaccgccgt
     1921 cgacgactcc ggtccggccc gagcgggcgt gacgttctgc ggcacgaccg gccactacat
     1981 catctggtac tacgacgcga acaaccagta ccgggagctc tggaactcct gcatctaggt
     2041 caccgacaga gggggccccg tccaatccgg acggggcctt ttcgcgagtg ctccctcgat
     2101 aggatgcgcc cgtgatcccc cgctacagcc gcgaagagat ggcggcgatc ttctccgagc
     2161 gcgccaagtt ctcgcgctgg ctcgacgtcg agctcctcgc cgtcgaggcc agggtgcgcc
     2221 tcggcgaggt ccccgtcgaa gacctcaccg agatcaagga gaaggcggcg ttcgacgtcg
     2281 cgcggatcga ggagatcgag gccgtccgcc accacgacgt cgtcgcgttc gtcgagaacg
     2341 tccgcgagaa cgtgggcgag gccgggcgcc acatccacta cgggatgacg tcttcggacg
     2401 tgctcgacac ggcgaacgcg gtcgcgctcc gcgacgccgg cgacctcctc gtcgaagaga
     2461 ccgggcggct caccgagacc gttcgccgca aggccctcga gcaccgcgac accctcatgg
     2521 ccggccgcac ccacggcgtg cacgccgagc ccacgacgtt cggcctcaag ctcgccggct
     2581 ggacgttcga gctcgcccgc gaccgcgacc gcctgagaag ggcacgcgac gaggtcgcgg
     2641 tcggcaagct ctccggcgcc gtcgggtcgt actcgcagct cccgccggac gtcgaggcct
     2701 acgtctgcga ccgcctcggc ctgaaggtcg aggacgccgc gacccaggtg atcccccggg
     2761 accgtcacgc agacttcctc ggcgcgatcg cgctcaccgg cgcgagcctc gaacggttcg
     2821 cccaggagat ccgccacctc cagcgcaccg aggtccgcga ggtcgaagaa ccgttcgcgc
     2881 agggccagaa gggctcgtcg gcgatgcctc acaagcgcaa cccgatcgtc tgcgagcgga
     2941 tctgcggcca ggcccggctg ctcagggcct acgcgatgac cggtttcgag aacgtagccc
     3001 tgtggcacga gcgcgacatc tcgcactcgt cggtcgagcg cgtgacgttc ggcgacgcct
     3061 gcgtgctgct cgactacatg ctggcgaaga tgcggtgggt cgtcgacggc atggtcgtcc
     3121 acgccgaccg gatgcggcgc aacctcgagg cgtcgtgggg gctcgtccac tcgcagggcg
     3181 tgctcacggc cctgctcggc aaaggcaccc tccagcgcga ggaggcgtac gcgctcgtcc
     3241 agcgcaactc gatgacggcg tgggacgaag ggcgcccgct gaaggagctc ctgaagtcgg
     3301 acccgcaggt gaccgagcac ctcgaccccg acgaaatcga ggcctgcttc gacccctcgg
     3361 gctatcttga gaaggcggaa gtggtctggg aacgattgga ggagctctga gtctggcaac
     3421 ccccgtccac gtgggttccg gcaaggtccg cgagatctac cgggtggacg acgagaccct
     3481 gctcctcgtc gcgaccgacc gcatcagcgc ctacgacgtc gtgctcccgc agcagatccc
     3541 cgacaagggc aaggtcctca ccggcctcac ccaccactgg ctcacgacga tgtcgtcgat
     3601 ctgcccgaac cacttgatct cggtggcgga gaaggacctg cccgacgtag gcattacgaa
     3661 cgatcgcctc ggcgatcgtc catgggcaga cgacctcgcg gggcgcgcca tgctctgcaa
     3721 ggccgccgag cccctgccga tcgagttcgt cgtgcgcggc tacctctccg gctccggctg
     3781 gcgcgagtac gtcgagcagg ggtcggtttg cggcatcccg cttcccgagg gcatgaagga
     3841 gtcggaccgc ctgccccggc cgctgctgac accggcgacg aaggccctcg aggggcacga
     3901 cgagaacgtg accgaggaac gcgccgccga gatcgcggga aaggaccgct acgacgccgc
     3961 caaggagtac gcgctggccc tgtacgcgac cgccgccgag caggccctcg agcgcggcgt
     4021 gatcatcgcg gacacgaagt tcgagttcgg cgtccacgac gacgaggtca tcctgatcga
     4081 cgaggtattg acgccggact cgagccgctt ctggccgcag gaccagtacg cgcccggcgg
     4141 cccccagccg tcgttcgaca agcagtacgt gcgcgattgg ctcgactcga cggggtggga
     4201 ccattcgccg cctccgccgg acctgccggc ggacgtcgtc gaggccacgc ggtctcgtta
     4261 cgtcgaggcg tacgagaagg tcaccggcaa cgcgtgggac cactgggacg ccgtcgtgac
     4321 cggcggcgcc gcatgagctc cgccgtctcc tccagccagg gcccggcgcg actcggcgcg
     4381 accgtccgcg gcgtgctggt ttcaccgggc tcggcattcg tccccgcgct gcgacgcgtc
     4441 gagacgtcgc aagagggcac ctcgacgtac gtgctcgcgg cgctcggcgg ggcggccgcg
     4501 atgctgctgt ggctcaaggt ccgcgccctc gtcgggttcc gctcgctggc gccgtcggac
     4561 ttctcgtgga gctacttcgt gatctcgctc gtagccgggg cgatcgtcgc ggtgctggcg
     4621 caggtcgtgt ggagcttcgt cggcccggcc gcagggggcc cggactccgg gtcgccccgt
     4681 tcgttccgcg cggtgtgggc gctgtccgcg ttcccgcagg tcccggccct gctcgtcctc
     4741 gcgccgatcg acctcttgct ggtcgggacc caggcattca cggcgagcga caccggcgac
     4801 tcggttgcac tcgcgtggct tgcgctgtcg accgcggggg ccgtcagctt ggcggcatgg
     4861 tccgcgtacg tgttcgcgaa ggggacgcac gtcgcgacgc gcgcggcgcc gcgtcggacg
     4921 gccggggtcg tcgccgcggg agtgctctgc ctcgggggcg tcgccgcgct cctcttcggt
     4981 gctctcgcag ggttggggga ggtgctgtcg tgacgttcac cgcacacgtc aacgtgatgc
     5041 tcaagccggg gatcgccgat ccgcagggac agacgatcga gaaggcgctg ccggtgctcg
     5101 ggtacgaggg cgtcggcgac gtacgggtcg gcaagaggat cgagctgcga ctcgacgcgg
     5161 ccgacgagtc cgacgcgcgc gacaaggtcg acgcgatgtg caagcagctc ctgtcgaacc
     5221 cggtgatcga gtcgtacgag gtcgatctca catgacggca acggtcggcg tcgtcgtctt
     5281 cccgggctcg aactgcgacc tcgacgcgca gcgcgcgttc gcagacctcg gcgcggacgt
     5341 gcggatggtg tggcacgagg agacctcgct cgacgggatc gatctcgtcg tgctgcccgg
     5401 aggcttcgcg cacggcgact acctccgcac cggcgccctc gctcgcttct ctccggtgat
     5461 ggatgcagtg atcgagcacg cggagcgcgg cggtctagtc gcggggatct gcaacggctt
     5521 ccagatcctc tgcgaggccg ggatgttgcc cggtgccttg cgcaagaacg ccggactgaa
     5581 gttcctgtgc aagtgggtcg acctccgcgt cgagaacgcg tcgaccccgt tcacggcgcg
     5641 cgccgagccg ggccagatcc tgcgcatccc gatcaaccac ttcgagggca actggtactg
     5701 cgacgcggac cagctcgaag agctgaaggc gaacgaccgc gtcgtcctgc gctacgccga
     5761 caaccccaac gggtcgctag acgacgtcgc ggggatcgtg aacgagcgcg ggaacgtctt
     5821 cgggctgatg ccgcaccccg agcgcgcctg cgagtcgctg ctcggctcgg aggacggccg
     5881 gctgatcctc gggtcgatgc tcgatcacgt ggccgcgcag cacgagctgt ctgtggccgg
     5941 ctgagcgtgg cactgcaccg cgacctcggc ctcaccgacg aggagctcga agcgatccgc
     6001 gaccgcctcg gccgcgaccc cagctacacc gagctcgcga tgttctcggt gatgtggagc
     6061 gagcactgct cctacaagtc gagccgcgtg cacctcaaga cgatgcccac cgagggcgcg
     6121 cacgtcgtgg tcgggccggg cgagggggcc gggatcgtcg aggtcgcacc cggcgtgcgg
     6181 atcgcgtgga aggtcgagtc gcacaaccac ccgtcgttcg tcgagccgtt caacggcgcc
     6241 gcgaccggcg tcggcgggat cgtgcgcgac atcctctcga tgggcgcgcg tccgattgcg
     6301 ctgatggact cgctgcggtt cggccccctc gacgatgcaa ggacccgtta cctcgtcgac
     6361 ggcgtcgtcc acggcatctc ctcgtacggc aactcgatcg gcgttccgac ggtcggcggc
     6421 gagacggtct tcgaggactg ctacgccgag aacccgctcg tcaacgtcgc gtgcctcggc
     6481 atcgtcgagg acgcgctgat gcacgggcgc gccgaggggc cgggcaaccc cgtcgtgctg
     6541 ttcggctcgt ccacgggacg ggacggcatc ggcggcgcga gcatcctcgc ctcggccgag
     6601 ttcgacgaag actccttgga gaagcgcccg tcggtccagg tcggcgaccc gttcaccgag
     6661 aagctcctga tcgaggcgtc cctggagctg atccgccgcg ggctcgtcgc gggcttccag
     6721 gacctcggtg ccggcggcat ctcgtgcccg acgtcggaga tgaccgctaa ggccggcacc
     6781 ggcatggtcg tcgacgtcga caaggtccac cggcgcgagc cgggcatgga gccgttcgag
     6841 gtcatgatct cggagtccca ggagcgcatg ctcgcggtcg tcgagcccaa ggacctcgac
     6901 gacgtgctcg aggtgtgccg ccgctggggg ctcgaggcgt cggtcctcgg cgaggtcacc
     6961 aagagcgggc ggctcgaggt gatcgccggc ggcgaggtcg tcgccgacgc gcccgccgag
     7021 gcgctcgcgg aggagggccc ggtctaccag cgacccctcg agcgccccgc gtggatcgac
     7081 gacgtgcagg ccgcaccggc gggcgac
//
LOCUS       DASQDO010000518         7495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_360129, whole genome shotgun sequence.
ACCESSION   DASQDO010000518 DASQDO010000000
VERSION     DASQDO010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360129"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VEV43_14220"
     CDS             <1..946
                     /locus_tag="VEV43_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018842467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYP24722.1"
                     /translation="GLSLEGAPTRITDLPEHAALDEPSRHVYKSLGIDTVLVSPSGAA
                     ELAIGATKAALDAAGLPPDEVDALVTVQGRVPELFMASEATKVQAGVGAARATTFAVA
                     DLGCVSINSAFLVARALLGSNPSWRNVVIAHGSKPPAPRRFRYPVTVNGDGGVAVVMT
                     RDALPRILDVAVETNGEYWDLYRVDFKDKPVREWAEECKSLKTYSFKLAIESRNRFAA
                     LNETVLARAGETMADVDHFVMQNLSAGAFRFYEEFFKIEFAKACKVNLARYGHLGSMD
                     VPLNLHTGIETGEFSAGDLVLVMNNSPVAAWSTMLVQT"
     gene            943..1968
                     /locus_tag="VEV43_14225"
     CDS             943..1968
                     /locus_tag="VEV43_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HYP24723.1"
                     /translation="MTRPTVKCLVWDLDNTLWRGTLLEDPSVTIDPAAVAIVQELDRR
                     GILNSIASRNEESLALEVLREHGLDEYFLFPQINWNAKDVSVKAVADALNVGIDTLAF
                     VDDQPFDRAEVAFSLPEVRCYSPDDLPGLLDLPDFNPPFVTPESAQRRLMYLADAART
                     QEEAEFSGPKEEFLSSLTMRFTISEAREDDLQRAEELTVRTNQLNTTGRTYSYDELAG
                     FMESDAHLLLVAELEDKHGTYGKIGLALVETGADVWVLKLLLMSCRVMSRGVGTVMLN
                     HVMREAAAAGAKLRAELVPNDRNRMMLITLRLGGFKECGREEATLLLESTSAPPPPPD
                     YLDLIVS"
     gene            complement(1961..2179)
                     /locus_tag="VEV43_14230"
     CDS             complement(1961..2179)
                     /locus_tag="VEV43_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HYP24724.1"
                     /translation="MSDAEDKTIYDVVVNDEEQYSIWPAHKDLPAGWRKAGKTGPKQE
                     CLAYVEEVWTDMRPKSLRDRMDAGSSPS"
     gene            complement(2182..3459)
                     /locus_tag="VEV43_14235"
     CDS             complement(2182..3459)
                     /locus_tag="VEV43_14235"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYP24725.1"
                     /translation="MSFARRLKQARLSAGMTQAQLAEPMYTHAYVSTLEAGRRQPSPT
                     AAKHFAQKLGMDVDELLTGRPPHLAASLRLTLQEARVALSRGEYEETDRIADKAVKDA
                     KKYRLVRLHASGLELLALAAERRSQLGRAQELYQEIEELLREEPLTALADARAGRIRC
                     LQMDGDPRYAIYLGESLLEQMRQKRIDDPAARVRIMAPMTQAYFETGMRRQASAVAEE
                     LLRLAPRVQDPFNLAVMHMNVSRVLMSDGHVADADESLRRAEDYFKTLDLQTELGRAH
                     LARGYLLLQDGRLDEAEEELNSALAIFELTSSKVDEGRTYAELGRLSRLRGDTRAAID
                     RLNKATTLLKDAKDAAEQAIVHREMGLALAETDTGAAQKSLRKAIVLFNKIDDVVEGA
                     KTYRLLGEFLEVEREADAAKSAYLEGLRLLERM"
     gene            3829..4386
                     /locus_tag="VEV43_14240"
     CDS             3829..4386
                     /locus_tag="VEV43_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24726.1"
                     /translation="MTPRKPGPRLLCTILATLFVAAGAAPARADLWTGSCVLRVTIDF
                     RAPVRPPLSAPNYDLSAEPGADLDLTQGGMQSCVRTLSSQPLGSTSAEGWGTATAWSC
                     GTTVARGAWTQSFDEEGPQEFSGTHVLTGTWGAWTLHIQSHDLHVIGVGEFTLATTDA
                     GKTPTCATAALDSVTLVGTIVFQDP"
     gene            complement(4391..6325)
                     /locus_tag="VEV43_14245"
     CDS             complement(4391..6325)
                     /locus_tag="VEV43_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24727.1"
                     /translation="MNPGRIDLTDAALVQGALGDDADAFGHLYRRFYPRLVRLVLRKT
                     GDRVLAEDVAQDTLLKALDKLDTFDRTRPLWPWLKVIATNLAVDAGRKRSREVEWDPG
                     DAAGVAAEELPSCEDGMVLAQVLSNLPDRQRVAVSLRYLEDWESSEAASFLGLSIPAF
                     EQLLFRARKRARLEYSRIAQGALGVLGLPARWLRQEVSRFGARVPGARRVVEAASQLG
                     PVTWSQAVAGSVALLVALPGAAGNGARDPDLPERHGVHVATRAGDRSDEGRAPATRSS
                     REAVAAQNRTAGSADPAPPPSHEEPHRTPGLGEMAKDVTDGSHRVQEPEDATIMSLAF
                     VPGSGGKRAYALGSAHCGFGACPSVLFATRDGGGSWRRLPASGLSGYELEVAPGTHGK
                     KLFAMSPAGLQVSTDGGRTFALAGLPTVPPLTGSMAISPGFDKGHPIMLIGGDWLVRY
                     DDSDASTMPDASTPFNGPFEPVFAPDYPSDPRFLLGASRLEDGWLQPTVYACRESGCT
                     WAHLPDAQGRPTVRPRPDYARSNVVYAFASNALYRSDDVWGYTRLATPWPESSRLSDL
                     AVVDNDGTLFAAVHSVGGKGGEGLFRSEDGGESWSPVRSPVFARGAISVVADGERMIV
                     ALPQGLACSADGGRSWDLRC"
     gene            6503..>7495
                     /locus_tag="VEV43_14250"
     CDS             6503..>7495
                     /locus_tag="VEV43_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24728.1"
                     /translation="MIDSARVESRAPRALTSADIASLHASLDPAVVSAEAARVLVEVT
                     GAASCALWTADAGTTTALAAAGPEGLRSFAGRRVARPAAFDDAIAARRPVVVPSIAGA
                     ALVAGEDDPIVLLALPRAMDVPAVAALGAPATVHDLGEVARSAGAALGNAFEIAEART
                     RVERLEVLHDLGAALVEAGGTEALLERLNRLLRGRGIEVEGLAWRSRALTRRVGGSEP
                     TAQERALLRDGGVATTLADGRLAVAVRTGDEALGSMRVRAAGDPDELPFLELVAAGVA
                     EVAARMSLRAEVEEASRGAALANERDRIAASLHDTAGQLFVAIRLLARREAEQLP"
BASE COUNT         1072 a         2609 c         2639 g         1175 t
ORIGIN      
        1 cggcctctcc ttggagggcg cgccgacgag gatcaccgat ttgcccgagc acgccgcgct
       61 cgacgagccg tcgcgtcacg tctacaagag cctcgggatc gacacggtcc tcgtgtcgcc
      121 ttcgggtgcg gcggagctgg cgatcggggc gacgaaggca gcgctcgacg ccgcaggact
      181 cccgccggac gaagtcgacg cactggtgac cgttcagggt cgcgtgccgg agctgttcat
      241 ggcgtccgag gcgacgaagg tccaggccgg cgtgggggcc gcgcgcgcga cgacgttcgc
      301 ggtcgcggac ctggggtgcg tgtcgatcaa ctcggcgttc ctggttgcgc gagcgctgct
      361 cggctcgaac ccgtcgtggc gcaacgtcgt gatcgcgcac ggcagcaagc ctcccgctcc
      421 ccgccgcttc cgctaccccg tcaccgtcaa cggcgacggt ggcgtcgcgg tcgtcatgac
      481 gcgcgacgcc ctgccgcgca tcctcgacgt cgccgtcgag accaacggcg agtactggga
      541 cctctaccgg gtggacttca aggacaagcc cgttcgagag tgggccgagg agtgcaagag
      601 cctcaagacg tattcgttca agctcgcgat cgagagccgc aaccgcttcg ccgctctgaa
      661 cgagaccgtg ctggcgcgtg cgggcgagac gatggcggac gtcgaccact tcgtgatgca
      721 gaacctctcc gccggcgcgt tccgcttcta cgaggagttc ttcaagatcg agttcgccaa
      781 ggcgtgcaag gtgaacctgg cgcgctacgg gcacctcggc tcgatggacg tgccgctgaa
      841 cctgcacacc gggatcgaga ccggcgagtt ctccgccggc gacctcgtgc tcgtgatgaa
      901 caacagcccg gttgcggcgt ggagcacgat gctggtgcag acgtgacgcg cccgaccgtg
      961 aagtgtctcg tgtgggacct cgacaacacg ctgtggcgcg ggacgttgct ggaggacccc
     1021 tcggtgacga tcgacccggc ggccgtggcg atcgtccaag agctggaccg gcgcgggatc
     1081 ctgaactcga tcgcgagccg caacgaggag tcgctcgcgc tcgaggtgct gcgcgagcac
     1141 gggctcgacg agtacttcct gttcccgcag atcaactgga acgcgaagga cgtctcggtg
     1201 aaggcggtgg ccgacgcgct caacgtcggg atcgacacgc tcgcgttcgt ggacgaccag
     1261 ccgttcgacc gggcggaggt cgcgttctcc ctgcccgagg tcaggtgcta ctcgccggac
     1321 gacctcccgg ggttgttgga cctccccgac ttcaacccgc cgttcgtgac gccggagtcg
     1381 gcgcagcgga ggctcatgta cctggccgac gcggcccgca cgcaggagga agccgagttt
     1441 tccggcccga aggaggagtt cctctccagc ctgacgatgc ggttcacgat ctcggaggca
     1501 cgcgaggacg acctgcagcg ggcggaggag ctgacggtcc gcacgaacca gctcaacacg
     1561 accgggcgca cgtactccta cgacgagctg gcgggcttca tggagtcgga cgcgcacttg
     1621 ctgttggtcg ccgagctgga ggacaagcac ggcacgtacg ggaagatcgg gctggccctg
     1681 gtcgagacgg gggccgacgt gtgggtgctg aagctcctgc tgatgtcgtg ccgcgtgatg
     1741 tcccgcggcg tgggcacggt gatgctgaac cacgtgatgc gcgaggcggc cgcggccggg
     1801 gcgaagctga gggccgagct ggtgccgaac gaccgcaacc ggatgatgct gataacgctg
     1861 cgcctaggcg gcttcaagga atgcggccgc gaggaggcga cgctgctgct ggagagcacg
     1921 agcgcccccc cgcccccgcc ggactacctc gacttgatcg tcagctaggc gaagagcccg
     1981 cgtccatccg gtccctgagg ctcttgggcc gcatgtccgt ccacacttcc tcgacgtagg
     2041 ccaggcactc ctgcttcggg ccggtcttgc cggccttgcg ccagcccgcg ggcaggtcct
     2101 tgtgcgccgg ccagatcgag tactgctcct cgtcgttgac gacgacgtcg tagatcgtct
     2161 tgtcctcggc gtcgctcatc gtcacatcct ctcgaggagg cgcaggccct ccaggtacgc
     2221 gctcttcgcc gcgtcggctt cgcgctctac ctccaggaac tcgcccagga gccggtacgt
     2281 cttggccccc tcgacgacgt cgtcgatctt gttgaacagc acgatcgcct tgcgcaggct
     2341 cttctgggcc gcgccggtgt cggtctcggc aagagcgagg cccatctcgc ggtggacgat
     2401 cgcctgctcg gccgcgtcct tcgcgtcctt cagcagcgtc gtggccttgt tcagccggtc
     2461 gatcgcggca cgcgtgtcgc cgcggagacg ggagagacgc cccagctctg cataggtacg
     2521 gccttcgtcg accttgctcg acgtcagctc gaagatcgcg agcgcgctgt tcagctcctc
     2581 ctcggcctcg tcgagacgcc cgtcctggag gaggaggtag ccgcgggcga ggtgggcacg
     2641 cccgagctcc gtctggaggt cgagcgtctt gaagtagtcc tccgcccgcc gcagcgactc
     2701 gtcggcgtcg gctacgtgac cgtccgacat cagcacacgc gacacgttca tgtgcatgac
     2761 ggcgaggttg aacgggtcct ggacccgcgg cgccagccgc aggagctcct cggccaccgc
     2821 gctcgcctga cgccgcatgc cggtctcgaa gtaggcctgg gtcatcggcg ccatgatccg
     2881 gacgcgcgcc gccgggtcgt cgatgcgctt ctgacgcatc tgctcgagca gtgactcgcc
     2941 gaggtagatc gcgtagcgcg ggtcaccgtc catctgcagg cacctgattc gtccggcgcg
     3001 ggcgtccgcc agcgcggtga gcggctcctc gcggagcagc tcctcgatct cctggtagag
     3061 ctcctgcgcg cggccaagct ggctgcgccg ttcggcggcc agcgcgagca gctcgaggcc
     3121 cgacgcgtgc aggcggacga gccggtattt cttggcatcc ttcaccgcct tgtcggcgat
     3181 gcggtcggtc tcctcgtact caccgcgcga cagcgccacc cgcgcctcct gcagcgtgag
     3241 ccgcaacgat gcagcgaggt gcggaggcct ccccgtcagt agctcgtcga cgtccattcc
     3301 cagcttctgg gcgaagtgct tcgccgcggt gggcgacggc tgacgccggc cggcctcgag
     3361 cgtgctgacg tacgcgtgcg tgtacatcgg ctcggcgagc tgcgcttgtg tcattccggc
     3421 cgacaaacgc gcctgcttga gccttctcgc gaagctcatc aatcctcctg gggatcgggt
     3481 tcgtaacgac tagttaagca actgaagcgt accgcacacg ggcttcctgg gtggttaagc
     3541 ccgggaatgt cctggtttgt aacagcggag tggacactcg aaacaccaaa tttaatatcg
     3601 tctgcagtcc cggtcacttg acccgacgaa gggagacgat gtgaagacac gtcttgtcaa
     3661 ggttgtgaca gttctgacga tcctcgcggc cagcggcctc gccgactttc cgctggggat
     3721 tctcggctgg cccaggtgct gctgaagtag ccggcactcc ttccccggct tgctcgtttt
     3781 gctcgccggg gaaggagttc tcgactcggc ggccaatggg ctctgcctat gactcctcgg
     3841 aagcccgggc ccagactgct ctgcacgatc ctcgccacgc tcttcgtagc cgcaggcgcc
     3901 gctccggcgc gggcagacct ttggaccggc tcctgcgtgc tccgggtgac gatcgacttc
     3961 cgcgcccccg tccggccgcc gctctcggcc ccgaactacg acctctccgc ggagccgggc
     4021 gcagacctcg acctgacaca gggcggcatg cagtcgtgcg tgcgaaccct gagcagccag
     4081 ccgctcggca gcacgtccgc cgagggctgg gggactgcga cggcctggtc ctgcggaacg
     4141 acggtggctc gcggtgcgtg gacccagtcg ttcgacgagg agggcccgca ggagttctcc
     4201 ggcacccacg tcttgacggg gacctggggc gcgtggacgc tccacatcca gtcgcacgat
     4261 ctccacgtga tcggcgtcgg tgagttcacg ctcgccacca cggacgccgg caagacgccg
     4321 acatgcgcta ccgcggccct cgattcggtc acgctggtcg ggacgatcgt gttccaggat
     4381 ccctgacgga tcagcagcga aggtcccagc tccggccgcc gtccgccgag caggcgagac
     4441 cctgcggcag cgccacgatc atccgctcgc cgtccgcgac caccgagatc gcgccgcggg
     4501 cgaagaccgg tgagcggacc ggggaccagg attcgcctcc gtcctcgctg cggaagaggc
     4561 cctcaccgcc ctttcctccg accgagtgga cggcggcgaa gagcgtcccg tcgttgtcca
     4621 cgaccgcgag gtcgctcagg cgcgagctct ccggccacgg cgtcgcgagc cgcgtgtagc
     4681 cccacacgtc gtcggagcga tagagcgcgt tcgacgcgaa ggcgtacacg acgttcgacc
     4741 gcgcgtagtc cggccgcgga cggaccgtgg gacgcccttg ggcgtccggg aggtgggccc
     4801 acgtgcaccc cgactccctg cacgcgtaca cggtcggctg tagccacccg tcctcgaggc
     4861 gcgaagcacc gagcaggaag cgcgggtccg acgggtagtc gggggcgaac accggctcga
     4921 acggcccgtt gaacggggtc gaggcgtccg gcatcgtgct ggcgtcggaa tcgtcgtagc
     4981 ggaccagcca gtcgccgccg atcagcatta tcgggtgccc cttgtcgaac ccgggcgaga
     5041 tcgccatcga ccccgtgagc ggcgggaccg tcgggagccc cgccagggcg aacgtccggc
     5101 cgccgtccgt gctgacctgg agccccgcgg ggctcatcgc gaacagcttc ttcccgtggg
     5161 tccccggagc gacttcgagc tcgtagcccg agaggccgct ggccggcagc cgtcgccacg
     5221 acccgccgcc gtcgcgggtc gcgaacagga cggacgggca agcgccgaag ccgcagtgcg
     5281 cgctgccgag cgcatacgca cgctttccgc cggagcccgg cacgaacgcg agcgacatga
     5341 tcgtcgcgtc ctccggttcc tggacgcgat gcgacccgtc ggtcacgtcc ttggccatct
     5401 cgccgaggcc gggcgtccgg tgcggctctt cgtgcgaagg gggcggagcc ggatccgcgg
     5461 agcccgccgt tcggttctgc gcggcgactg cttcgcggga tgagcgcgtc gcgggggccc
     5521 ggccctcgtc ggagcggtcg ccggctcggg tggcgacgtg cacgccgtgt cgctcgggga
     5581 ggtccgggtc gcgcgcgccg ttcccggcgg ctcccgggag ggcgacgaga agagccacgc
     5641 ttccggcgac cgcttgcgac cacgtcaccg ggccgagctg cgacgccgcc tcgacgacgc
     5701 ggcgggctcc gggcacccgg gccccgaacc gtgagacctc ctgtcggagc caccgtgccg
     5761 gaaggccgag cacgcccagc gcgccctgcg cgatccggct gtactcgagg cgcgcgcgct
     5821 tgcgggcgcg gaacaacagt tgctcgaaag ccggtatcga caggccgagg aacgacgcgg
     5881 cctcggaaga ctcccagtcc tccaggtagc gcagcgacac ggcgacacgc tggcggtccg
     5941 ggaggttcga caggacctgc gcgagcacca tgccgtcctc gcacgacggg agctcttcgg
     6001 ccgcgacgcc ggccgcgtcc ccggggtccc actcgacctc gcggcttcgc ttgcgccccg
     6061 cgtcgaccgc gaggttcgtc gcgatcacct tgagccacgg ccacagcggc cgggtccggt
     6121 cgaaggtgtc gagcttgtcg agagccttga gcagcgtgtc ctgcgcgacg tcctcggcga
     6181 ggacgcggtc tcccgtcttc cggaggacca gcctgaccag gcgcgggtag aacctgcggt
     6241 agaggtgccc gaaggcgtcc gcgtcgtcgc ccagcgcgcc ctgaacgagc gcagcgtcag
     6301 tcaggtcgat tctgcccgga ttcatctcgc cgcctggatg ccgtgggccc tgccccaccg
     6361 cgaggcgtcc ggccctggat cggtttgctt tttatcagac ccttgacgcg aaggcaatgc
     6421 gcttgccctg ctgggacaga cggggtcgtc gagcgtaagg aattgtgcgg ttcgtccgta
     6481 ttgccccccg atgagagctg gtgtgatcga ttcggcgcgg gtcgagagcc gcgcgccgcg
     6541 agccctgacg tctgcggaca tcgcgtcgct gcacgcctcg ttggaccctg cggtcgtctc
     6601 cgccgaggcc gcccgggtgc tcgtcgaggt gacgggtgcg gcgtcgtgtg cgctgtggac
     6661 ggcggacgcc ggaacgacca cggcgctcgc cgccgcgggg cctgaaggcc ttcgctcctt
     6721 cgccggccgt cgtgtcgcgc gcccggccgc gttcgacgac gcgatcgccg cgcgtcgccc
     6781 ggtggtcgtg ccgtcgatcg ccggcgctgc tctcgtcgcc ggagaagacg acccgatcgt
     6841 cctcctggcg ctcccccgcg ccatggatgt tccggcagtc gcggcattgg gcgcgcccgc
     6901 caccgttcac gacctcgggg aggtagcgcg cagcgcgggg gccgcgctcg ggaacgcgtt
     6961 cgagatcgcc gaagcccgca cgcgggtcga gcgcctcgag gtgctgcacg acctcggcgc
     7021 cgccctcgtc gaggcgggag gcaccgaagc gctcctcgaa cgactcaacc gcctgctgcg
     7081 cggtcgcggg atcgaggtcg agggcctcgc ttggcgaagc cgtgcgctca cccggcgcgt
     7141 gggcgggagc gagccgacgg cgcaggaacg tgcgctgttg cgtgacgggg gcgtggcgac
     7201 gacgctcgcc gacgggcgcc tcgcggtcgc ggtgcgaacc ggggacgagg ccctcggctc
     7261 gatgcgcgtg cgcgcggccg gcgatcccga cgagctgccg ttcctcgagc tcgtcgcggc
     7321 cggcgtcgcg gaggtggcgg cgcgcatgtc gctgcgcgcc gaggtcgagg aggcgtcgcg
     7381 cggcgcggct ctcgcgaacg aacgcgaccg gatcgcagcg agcttgcacg acacggcggg
     7441 gcaactcttc gtggcgatcc gcctgctcgc gcgccgggag gccgagcagc tcccc
//
LOCUS       DASQDO010000519         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_605198, whole genome shotgun sequence.
ACCESSION   DASQDO010000519 DASQDO010000000
VERSION     DASQDO010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605198"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VEV43_14255"
     CDS             <1..734
                     /locus_tag="VEV43_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HYP24729.1"
                     /translation="LLPDLVPQSPYEVVGPTTGFSLGLGVDAPLLVDGCYPEERVRKG
                     AERCLRFDTIVGNAGAGAFELAYKVDKRETVAAVQRIYRADGSFGDRFAVRSELHPTH
                     AHFLVLDFDVSRLWKEGPDGERVGEPVDHGDKNGFCPEDSEPLGGRPADGPRHYSCFT
                     ADEPDQRGFQVVGISAGWQDVYTANLPDQFVEISGVPDGQYVLEIELDPRRVFVESDD
                     SNNAICLLIRLDGFDAEIVEPEIPC"
     gene            1031..1312
                     /locus_tag="VEV43_14260"
     CDS             1031..1312
                     /locus_tag="VEV43_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24730.1"
                     /translation="MTERELDLSEEGRARRRFLKQAGTVAWATPFLVTMMSRGAAAHH
                     NVCGTAQPAPPPYFVTCGQSDPCGSGFYCSPQSPDWAPGDPCHCTEIPS"
     gene            1418..2395
                     /locus_tag="VEV43_14265"
     CDS             1418..2395
                     /locus_tag="VEV43_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP24731.1"
                     /translation="MRAQALPVRQVTMPTPYAVGPVNAYLIEAEPLTLVDAGINTPDA
                     RNALVLALASAGYFVESLERILVTHAHPDHYGLVPYLQEVSGATVYFPEREIARVRDS
                     QMLFEVGRLLVQAGMPLELLFKMDQQRKKDPAPKVPHDAVVPVKDGDEFHFESPSDTR
                     FTLRALLMPGHTGGHVVYFDDDSKTLFAGDQLLPDVSPNPLLEPSLDEPGQRRRSLQE
                     YLVSLERMAEMRIALAYPGHGAPVEDPQRLIEQTIEHHLKRKAQVAQRLGPDGKTPYQ
                     LAQEVYPHVTGYDTFLAVSEIVAHLDLVVHDGNAVVEEREGVTYYLGAP"
     gene            2484..3248
                     /locus_tag="VEV43_14270"
     CDS             2484..3248
                     /locus_tag="VEV43_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYP24732.1"
                     /translation="MEIVVCVKHIPDPNIPPEMDGHRLKREGVQGVLDPGDEFGVEAG
                     LQLKEQLGGNVTLVSMGPAAAMDAIKRGLSMGADKGVLVSDDALEGADALVTARVLAA
                     AIGRSEFDLVIAGVESTDGYTGTMPSTLAEFLGLPQVTFAKTIDCSDGRVKINRQTAD
                     GYHVVECDLPALITVTAGVNEPRYASFKGIMAAKKKPVETLSVADLGLSGDDVAIKQN
                     IVELAAAEERKAGEVIQDEGEGAVRIAEFLQEAKVI"
BASE COUNT          531 a         1143 c         1117 g          484 t
ORIGIN      
        1 ggctgctccc ggacctcgtc ccgcagtcgc cgtacgaggt ggtaggcccg acgaccgggt
       61 tctcgctcgg gctgggcgtg gacgcaccgc tgctcgtcga cggctgctac ccggaggagc
      121 gcgtgcgcaa gggggccgag cgctgccttc gcttcgacac gatcgtgggg aacgcgggcg
      181 cgggcgcgtt cgagctcgcg tacaaggtcg acaagcgcga gacggtggcg gcggtccagc
      241 ggatctaccg ggcggatggg tccttcggcg accgcttcgc cgttcggtcc gagctccacc
      301 ccacgcacgc gcacttcctc gtcctggact tcgacgtgtc gcggctgtgg aaggagggcc
      361 cggacgggga gcgggtcggt gagccggtcg accacggcga caagaacgga ttctgccccg
      421 aggactccga gccgctggga ggtcgtccgg ccgacggccc gcgccattac agctgcttca
      481 ctgcggacga gccggaccag cgcggctttc aggtggtcgg gatctcggcg gggtggcagg
      541 acgtgtacac agcgaacctg ccggaccaat tcgtcgagat ctcgggggtc ccggacgggc
      601 agtacgtcct ggagatagag ctagaccccc gccgcgtgtt cgtcgagtcc gacgactcga
      661 acaacgcgat ctgcctcctg atcaggctgg acggcttcga cgcggagatc gtggagcccg
      721 agatcccctg ctgaacgctc aggcctcgct gcgatcgaga acgtcgtcgg cagccacgag
      781 agcgaccgcc tgactgggac gccggtgaca ccaggctcga gggcggcggg ggccgggacc
      841 acttggacgg ggccgagggc atcgacgagc tggacggcgg tggcggcgac gatgacacct
      901 gcctgaacgg ggagctactg gaacgctgcg aatcgatggg gtagggggcc ggtcgaaccg
      961 gcaaaacgtc ttgtggggcg cggtccatcc tggatggcgt cgtcctaccg agcgcgtcag
     1021 gaggcacgga gtgaccgagc gtgagctgga cctttcggag gagggccgcg cccgcaggcg
     1081 cttcctgaag caggccggga cggtcgcgtg ggcgactcca ttcctcgtca cgatgatgtc
     1141 gcgcggggcc gctgcccacc acaacgtctg cggaaccgcc cagccggcgc cgcccccgta
     1201 tttcgtcacc tgtggccagt cggacccgtg cgggagcggg ttctattgca gtccacagag
     1261 ccccgactgg gcccccggcg acccctgtca ctgcacggag atcccctcgt agccgggctc
     1321 ggcccccgca ccgccggcta gtccacgttc gtcgagatcc gcgttcgtcc cgctatgcgc
     1381 gacaggatcg gaactcggcc ccgtaggctc ggcccccatg cgagcccagg ccctccccgt
     1441 gcggcaggtc accatgccca cgccttatgc cgtcgggccc gtcaacgcct acctgatcga
     1501 ggcagagccc ctcacgctcg tcgacgccgg catcaacacc cccgacgccc gcaacgcgct
     1561 cgtgctcgcc ctggcctcgg ccggctactt cgtcgaatcc ctcgagcgca tcctcgtcac
     1621 ccacgcgcac cccgaccact acgggctcgt gccctacctc caggaggtct ccggcgccac
     1681 cgtctacttc cccgagcgcg agatcgcccg cgtgcgcgac agccagatgc tgttcgaggt
     1741 cgggcggctc ctcgtccagg ccggcatgcc gctggagctc ctcttcaaga tggaccagca
     1801 gcgcaagaag gacccggccc ccaaggtccc gcacgacgcc gtcgtcccgg tgaaggacgg
     1861 cgacgagttc cacttcgagt cgccaagtga cacgcggttc acgctgcgcg ccctcctgat
     1921 gcccggccac accggcggcc acgtcgtcta cttcgacgac gactccaaga cgctgttcgc
     1981 gggcgaccag ctgctgcccg acgtgtcgcc gaacccgctg ctcgaaccct cgctcgacga
     2041 gcccggccag cgccgccgct ccttacagga gtacctggtg tcgctcgagc gcatggcgga
     2101 gatgcgcatc gccctcgcgt acccgggcca cggggccccc gtcgaggacc cccagaggct
     2161 gatcgagcag acgatcgagc accacctcaa gcggaaggcg caggtggcgc agaggctcgg
     2221 tcccgacggc aagacgccgt accagctcgc gcaggaggtc tatccgcacg tcaccggcta
     2281 cgacacgttc ctcgcggtct cggagatcgt ggcgcacctc gacctcgtcg ttcacgatgg
     2341 gaacgcggtc gtggaggagc gcgagggcgt cacctactat ctcggcgccc cctagagacg
     2401 gggggcgtta tctttgtgct tgctattgca agcaccccgg gcgtctgccc accagaccga
     2461 cccagcactc ctaggaggtc ccgttggaga tagtcgtgtg cgtaaagcac atcccggacc
     2521 cgaacatccc cccggagatg gacgggcacc ggctcaagcg cgagggcgtg cagggcgtcc
     2581 tcgaccccgg tgacgagttc ggcgtcgagg cgggtctcca gctcaaggag cagctcggcg
     2641 gcaacgtgac actagtctcg atgggccccg ccgccgcgat ggatgccatc aagcgggggc
     2701 tctcgatggg agccgacaag ggcgtgctgg tttccgacga cgcgctcgag ggcgcggatg
     2761 cgctcgtgac cgcccgggtc ctcgccgccg cgatcggccg gagcgagttc gacctcgtca
     2821 tcgcaggcgt cgagtcgacc gacggctaca cgggaacgat gccgtcgacg ctcgccgagt
     2881 tcctcggcct cccgcaggtc acgttcgcga agacgatcga ttgctccgac ggccgcgtga
     2941 agatcaaccg ccagaccgcc gacggctacc acgtcgtcga gtgcgacctg ccggcgttga
     3001 tcaccgtgac cgccggtgtg aacgagccgc gctacgcgtc gttcaagggg atcatggccg
     3061 cgaagaagaa gccggtcgag acgctgtcgg tcgccgacct cggcctctcc ggcgacgacg
     3121 tggccatcaa gcagaacatc gtcgagcttg ccgccgccga ggagcgcaag gccggcgagg
     3181 tcatacagga cgagggcgaa ggcgcagtgc gcatcgccga gttcctgcag gaagcgaagg
     3241 tgatctagcg atggcgaaga tctgggtgtt cgcag
//
LOCUS       DASQDO010000520         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1095260, whole genome shotgun sequence.
ACCESSION   DASQDO010000520 DASQDO010000000
VERSION     DASQDO010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095260"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VEV43_14275"
     CDS             <1..1249
                     /locus_tag="VEV43_14275"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HYP24733.1"
                     /translation="AFEGEVEAISSAEPRGAGVRLLTGGRVGFAYTTDLTEQGVAAVV
                     AQARENSASATPDDAADLAPAWEEAPEDIPGLVDEEHVATLPDAKAEFAIALEQATRA
                     VDTRIRTEEAIYSDSVSEVAIATSAGIAGSYRRTDAWCYSVAIAEDGDDTQVAFEFDL
                     ARGLSALDHESIARRAADRALSILGAQKIPSAKMPVVFDPYTSGQFLGVIAAALTGEA
                     VQKGRSLFAGKVGEQVAAPALSLVDDGRVAGAPGSAPWDAEGVPTRRTPVIEGGTLRS
                     FLYDVTSARREGRASTGNAARAGFKSAPHPSATNLAFDTTGQSRDEILRSAGRALLVQ
                     DFHGVHSGTNPISGDFSVGATGRLVEDGELTQPVREVTIAAPMLDILARITAVGTDRR
                     WLPFGGSYGGATTLVSEMTVAGA"
     gene            complement(1253..1843)
                     /locus_tag="VEV43_14280"
     CDS             complement(1253..1843)
                     /locus_tag="VEV43_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24734.1"
                     /translation="MGPRAVKMGYMRIFPLAVAFLVAASPAQAGAGAITATRVERFEV
                     AFLYKADRAYEEYVAVATRTTDPMSGDVLGVWVRAGIRPCSLDPEGALTCRGDLAEQE
                     VVRFEASEDMLDGTLVFRSPRGRTSRIVLRGDDPYAASGRSLPNECGGATFQRYEQAY
                     NASARGTMFGRKVTTQGDADPEAESMSRHLEIKGCA"
     gene            complement(1869..>3169)
                     /locus_tag="VEV43_14285"
     CDS             complement(1869..>3169)
                     /locus_tag="VEV43_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24735.1"
                     /translation="GEGSYAVVTDVSAFPAEGGQAVFEPGTAYEEAFAYGSVDAEANR
                     LVDLDRSAPIAHPAGAFVTAVSSATPAPTPTAAPSPGAEETEAEEPEAAEPAEPSEAA
                     AGEESSAEVILDPCEMVFDRTCLEVVTDTVGENPCDAFSGTGRTCDELVDELTGDPCG
                     LLFGKTCLDWIMEDVVGENPCDAFSGTGRTCDELVQDVANDPCGTLLGNTCLGWIVDV
                     IGENPCDAFSGTGRTCDELVQDLANDPCGTLVSTDCISYLLLLVDWAAETVGNASDPC
                     PAMTVEECTGVPEVNLDPEDLVIDGVHNSLTDDGDCSIGVAPPQKIQESATGAYYVMS
                     YGEANCPKYDDVEVTTCLQIKTPDGWKRKNCTSGTEFGQYDAAATLGPCAAKTHKYRT
                     KFTWLAFYITGAVDDPGADDAPSSGTRKVPLNGTKITCPP"
BASE COUNT          466 a         1110 c         1085 g          508 t
ORIGIN      
        1 ggcgttcgag ggcgaggtcg aggcgatctc gtctgccgag ccgaggggcg ccggcgtgcg
       61 tctcctcacc gggggccgcg tcgggttcgc ctacacgacc gacctcaccg agcagggggt
      121 ggcggccgtc gtggcgcagg cgcgcgagaa ctccgccagt gcgacccccg acgacgcggc
      181 cgatctcgcg ccggcgtggg aggaagctcc cgaggacatc cccgggctcg tcgacgagga
      241 gcacgtggcg acactcccgg acgccaaggc cgagttcgcg atcgctctcg agcaggcgac
      301 gcgcgcggtc gacacccgca tcaggaccga ggaggcgatc tactccgact ccgtcagtga
      361 ggtcgcgatc gcgacgtcgg ccgggatcgc cggctcctac cggcgcaccg acgcgtggtg
      421 ctactcggtc gcgatcgccg aggacgggga cgacacgcag gtcgcgttcg agttcgacct
      481 cgcccgcggc ctcagcgcgc tcgaccacga gtcgatcgca cggagggccg cggatcgcgc
      541 gctgtcgatc ctcggcgcgc agaagatccc ctcggcgaag atgccggtcg tcttcgaccc
      601 gtacacgtcc ggccagttcc tcggcgtgat cgccgcggcg ctgacgggcg aggccgtgca
      661 gaagggacgc tcgctgttcg ccggcaaggt cggcgagcag gtcgcggccc ccgcgctctc
      721 gctcgtggac gacgggcggg tcgccggtgc tcccggctcg gctccgtggg acgccgaagg
      781 tgtaccgacg cggcgcacgc cggtgatcga gggcgggacg ctgcgctcgt tcctctacga
      841 cgtcacgagc gcacggcgcg agggccgggc ctcgacgggc aacgccgcac gagcggggtt
      901 caagtccgcg ccgcacccgt cggcgacgaa cctcgccttc gacacgaccg gccagagccg
      961 agacgagatc ctccgcagcg cgggccgcgc tctgctcgtg caggacttcc acggcgtgca
     1021 ctcgggcacg aacccgatat cgggcgactt ctccgtcggc gccaccggac gtctggtcga
     1081 ggacggcgag ctgacgcagc cggtacgcga ggtgacgatc gcggccccca tgctcgacat
     1141 cctggcgagg atcacggccg tcggcaccga ccgacgctgg ctcccgttcg ggggctccta
     1201 cgggggggcg acgacgctcg tgtccgagat gaccgtcgcg ggggcctagg ccttacgcgc
     1261 agcccttgat ctccaagtgc cgggacatcg actcggcttc gggatcggca tcaccttgcg
     1321 tcgtcacctt tcggccgaac atggtccccc gcgcactcgc gttgtatgcc tgctcgtacc
     1381 tctggaaggt ggcccctccg cattcgttgg gaagcgaacg tccgctcgcg gcgtagggat
     1441 cgtcgccgcg cagcacgatc ctgctcgtgc gcccgcgggg gctccggaac accagggtcc
     1501 cgtccagcat gtcctcgctc gcttcgaagc gcacgacctc ttgctcggcc agatcgcccc
     1561 tgcacgtgag cgcgccttcg ggatccagcg aacaaggcct gattccggcg cgaacccaga
     1621 cgccgaggac gtcgccgctc atcgggtcgg tcgtccgggt tgcgaccgcc acgtactcct
     1681 cgtaggcacg gtccgccttg tacaggaacg cgacttcgaa tcgctcgacc cgcgtcgccg
     1741 taatggcgcc ggctccggcc tgagcagggg acgccgccac gaggaacgcg accgcgagcg
     1801 ggaagattcg catgtagccc atcttcaccg cccgcggtcc caaacctgca ttcctcaccc
     1861 gttcaggatc acgggggaca ggtgatcttc gtgccgttga gggggacctt cctggtccca
     1921 gaggacgggg cgtcgtcggc gcccgggtcg tcgacggcac cggtgatgta gaaggcgagc
     1981 caggtgaact tggtcctgta cttgtgggtc ttggcggcac agggcccgag cgtcgcggcg
     2041 gcgtcgtact gaccgaactc cgtgccgctc gtgcagttct tacgcttcca tccgtccggc
     2101 gtcttgatct gcaagcacgt cgtcacctcg acgtcgtcgt acttcggaca gttcgcttcg
     2161 ccgtacgaca tgacgtagta cgcgccggtc gcggactcct ggatcttctg cggaggagcc
     2221 acaccgatag aacaatcgcc gtcgtccgtc agggagttgt gcacaccgtc gatgacgaga
     2281 tcctcgggat cgagattgac ctcgggcact cccgtgcatt cttcgacggt catggcggga
     2341 cacgggtcgc tcgcgttgcc gacggtctcc gcggcccagt cgacgagcag gagcaggtag
     2401 ctgatgcagt cggtgctcac gagcgttccg caagggtcgt tcgcgaggtc ctggaccagc
     2461 tcgtcacagg tacggccggt cccgctgaag gcgtcgcacg ggttctcccc gatgacgtcg
     2521 acgatccagc ccagacaggt gttgccgagc agcgtcccgc agggatcgtt cgcgacgtcc
     2581 tgtacgagct cgtcacacgt tcgaccggtc ccgctgaagg cgtcgcacgg gttctcgccg
     2641 accacgtcct ccatgatcca gtccaggcac gtcttgccga agagcaagcc gcacgggtcg
     2701 ccggtcagct cgtccacgag ctcgtcacac gttcgaccgg tcccgctgaa ggcgtcgcac
     2761 gggttctcgc cgaccgtgtc cgtcacgacc tcgagacagg tgcggtcgaa caccatctcg
     2821 caggggtcga ggatcacctc tgcgctgctt tcctcgccgg cggcggcttc gctcggctcg
     2881 gcgggctctg cggcctccgg ttcttccgct tccgtctcct cggccccggg ggacggcgct
     2941 gccgtcggcg tcggagcggg agtcgcggac gagacggcgg tgacgaaggc gccggccggg
     3001 tgcgctatcg gagcgcttcg atcgaggtcc accagacggt tcgcctcggc gtcgacggac
     3061 ccgtacgcaa aagcctcttc gtaagccgtc ccgggttcga agacggcctg gccaccctcg
     3121 gccggaaacg ccgacacgtc ggtcacgacg gcgtaggagc cttcgccgt
//
LOCUS       DASQDO010000521         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_911387, whole genome shotgun sequence.
ACCESSION   DASQDO010000521 DASQDO010000000
VERSION     DASQDO010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911387"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VEV43_14290"
     CDS             complement(<1..570)
                     /locus_tag="VEV43_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24736.1"
                     /translation="MRKIFTTTPNRHESKPSGTRTLPRAVPSSSRSFTTRSWTASGCD
                     PPGGSGLGDGDGSAGGVGSTEVSGLGVGEGVGDGDGLGDGVGEGEGDGDEAGEGLGFG
                     DGVGLGAGDGLGEAEGGAAPSAHRDSGNDATSTTVRHAFTRPPDTVLPRYAGASTAPS
                     TSARATVTGEASGCALHASAAAPATCGAAI"
     gene            554..727
                     /locus_tag="VEV43_14295"
     CDS             554..727
                     /locus_tag="VEV43_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24737.1"
                     /translation="MKIFRNGDVVEKVETRATGRFRVKLRHENGRYVAVAPELVVGDS
                     DLCAQAKSKPRRT"
     gene            819..1367
                     /locus_tag="VEV43_14300"
     CDS             819..1367
                     /locus_tag="VEV43_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HYP24738.1"
                     /translation="MLLVDLAMRRGFKPAAAAGAGVATADFLYAVVAVLLGAAVADAV
                     ESFQDTLRIVSAMVLFGVAAHLLWSAIRSRAPVPADTPVPVAGAVATYVRFVGLTILN
                     PATITYFVALIVGLDRGDATAADKVLFVVGAFLASLSWQVWLAALGALLHKRIPESVR
                     WMFGAAGAVVVAGFAVRLIVSG"
     gene            complement(1351..2061)
                     /locus_tag="VEV43_14305"
     CDS             complement(1351..2061)
                     /locus_tag="VEV43_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24739.1"
                     /translation="MNDLESALGAMLEDRAGRIEPRPGHRDATLRGARRRRLLSVAAA
                     AFVPLVVAAGAFASIRSSENKPAPRPAAQEDGAPVAYSFASRRGDYPHVATGTFRNVD
                     WQLRVAGVNPGHDSAVRITLELQGPVRRVTTTSQRVFLGEELFVRYEDGSLLFDGRVA
                     VVFGAVDPEAEIVDVWTKGIESGERTFRAELFEGYDAKAGVHADYFLAFVPAHRTGLV
                     IAKDGDGNEVGAHPIPTR"
     gene            complement(2058..2570)
                     /locus_tag="VEV43_14310"
     CDS             complement(2058..2570)
                     /locus_tag="VEV43_14310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HYP24740.1"
                     /translation="MEADAAVSSAGGSRLEALYSRHSRSAIRLAFLMTGDEHQAQDIA
                     QEAFAKIAGRFADLRDPDALPGYLRTTVLNLSRGHLRRLRTQREYLKRTPSREPVVPD
                     DDPGRRDAMWRALQRLPYRQRAALVLRYYEDLSERQAADVLGCSVSAMKSLVTRGLEA
                     LRAEMEGDAR"
BASE COUNT          366 a         1023 c          947 g          427 t
ORIGIN      
        1 gatggctgcc ccgcacgtag cgggcgcggc cgcgctcgca tggagcgcgc aaccggatgc
       61 ctctcctgtg acggtcgccc gggcgctcgt cgacggcgcg gtcgacgcgc ccgcgtacct
      121 gggcaagacc gtgtccgggg gccgcgtgaa cgcgtgccgg accgtcgtgc tcgtggcgtc
      181 gttgccggag tcccggtgcg ccgacggagc cgcgccacct tccgcttcgc cgagcccgtc
      241 gccggcaccg agccccacgc cgtctccgaa gccgagccct tcgccggctt cgtctccgtc
      301 accttcgccc tcaccgacgc cgtctcccag cccgtcaccg tcaccgacgc cctctcctac
      361 gcccagcccg gagacctctg tcgaaccgac acccccggcc gagccgtccc cgtcgcccag
      421 ccccgagccg cccgggggat cgcagccgct cgcggtccac gaacgggtcg tgaagctccg
      481 gctcgacgac ggaaccgcgc ggggcagagt gcgcgtcccc gacggcttcg actcgtgccg
      541 gttcggggtg gtcgtgaaga tcttccgcaa cggcgacgtg gtcgagaagg tcgagacgcg
      601 cgcgaccggg aggttccggg tgaagctccg ccacgagaac ggtcgctacg tcgcggtcgc
      661 gccggagctc gtcgtcggcg actccgatct ctgcgcgcag gcgaagtcga agccccgcag
      721 gacctgacgc gcgcgccggt actctccggc gcatgaatta gttcctcgaa ggcgctctcg
      781 ccggcttcgg catagcgatc cccgtcggcg cgataggcgt gctgctggtc gacctcgcga
      841 tgcgccgcgg gttcaagccg gcggcggccg caggagccgg cgtagccact gccgacttcc
      901 tctacgctgt cgtcgccgtg ctcctggggg ccgcggtcgc cgacgcggtc gagtcgttcc
      961 aggacacgct gcggatcgtc tccgcgatgg tgctgttcgg ggtcgccgcg cacctcctgt
     1021 ggagcgcaat ccgaagccgt gcccccgttc ccgcggacac ccccgttccc gtcgccggag
     1081 ccgtcgcgac gtacgtgcgc ttcgtcgggc tgacgatcct gaacccggcg acgatcacgt
     1141 acttcgtcgc gctgatcgtc gggctcgacc gcggcgacgc gaccgcggcc gacaaggtcc
     1201 tgttcgtagt gggcgcgttc ctcgcgtcgc tgtcgtggca ggtctggctg gcagcgctcg
     1261 gcgcgttgct gcacaagcgc attcccgaga gcgtgcgctg gatgttcgga gcggcgggcg
     1321 ccgtcgtcgt agcagggttc gccgtacggc tcatcgtgtc gggatagggt gcgcgccgac
     1381 ctcgttcccg tccccgtcct tcgctatgac gagcccggtc cggtgtgccg ggacgaacgc
     1441 aaggaagtaa tccgcgtgca cccccgcttt ggcgtcgtaa ccctcgaata gctccgcccg
     1501 gaaggtccgc tcgccgcttt cgattccctt ggtccagacg tccacgatct cggcctccgg
     1561 gtcgaccgca ccgaacacca ccgcgacccg accgtcgaac aacaacgagc cgtcctcgta
     1621 tctgacgaag agctcctccc cgaggaagac ccgctgcgac gtcgtcgtca ccctgcggac
     1681 gggaccctgc agctcgaggg ttatacgcac ggcggagtcg tggcccgggt tgaccccggc
     1741 caccctcagc tgccagtcga cgttgcggaa agtaccggtg gcgacgtgcg ggtagtcgcc
     1801 ccgccgggac gcgaacgaat aggcgaccgg ggcgccgtct tcctgcgcgg cgggccgcgg
     1861 cgcgggcttg ttctcggagc tccggatcga ggcgaacgcc ccggcggcga cgacgagcgg
     1921 gacgaacgcg gccgcggcga cactgagcag acgcctgcgg cgcgcgccgc gcagcgtggc
     1981 gtcgcggtgg ccgggccgcg gctcgatcct ccccgcgcgg tcctcgagca ttgctcccag
     2041 cgcactttcg aggtcgttca tctcgcatca ccttccatct ccgcgcgcag cgcttcgagg
     2101 ccgcgcgtca ccaaggactt catcgccgag accgagcacc cgaggacgtc ggcggcctgg
     2161 cgctccgaga ggtcctcgta gtagcggagg acgagcgcgg ccctctgccg gtacgggagt
     2221 cgttgcagcg cacgccacat cgcgtcgcgc cgtcccgggt cgtcgtccgg aacgaccggc
     2281 tcccgcgacg gggtgcgctt caggtactcg cgttgcgtgc gcagacgccg gagatgcccg
     2341 cgcgagaggt tgaggacggt cgtccggaga tagcccggca gcgcatccgg atccctgagg
     2401 tcggcgaagc ggccggcgat cttggcgaac gcctcctgcg cgatgtcctg cgcctggtgc
     2461 tcgtcgccgg tcatcaggaa agcgagccgg atcgccgacc gcgagtgcct tgagtacaag
     2521 gcctcgagcc ggctgccgcc cgccgagctc actgccgcgt ccgcttccac caagaggctc
     2581 cccgtgacgg tctctactct gaagacgcgc gggggccgcg ggaggtcgcg gctagttcgc
     2641 cgtcgggtcc gcgctcatcg tcgagagcat cacgaactcg ccgcccttgc gtcgcagcac
     2701 gtcgcgccac agccggtccg ggtcgcgcgt gaacacgtcc tcggccctcg ggtcctcgat
     2761 cac
//
LOCUS       DASQDO010000522         6724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1033771, whole genome shotgun sequence.
ACCESSION   DASQDO010000522 DASQDO010000000
VERSION     DASQDO010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033771"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /gene="ppdK"
                     /locus_tag="VEV43_14315"
     CDS             complement(<1..1948)
                     /gene="ppdK"
                     /locus_tag="VEV43_14315"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HYP24741.1"
                     /translation="MTKWVYQFEEGDRSQKNLLGGKGANLAEMTNLGLPVPPGFTITT
                     EACNAYREGGQEFPNGLPDEVRENVKFLEEKMGRGLGDERDPLLVSVRSGAPFSMPGM
                     MDTVLNVGLNDESVAGLAQQTGDERFAWDSYRRLVQMYGKTVLGIEGDLFEDALEEAK
                     HKKKVSNDVDLDVRDLKALVKTFKGIVRKAGFAFPDKPEEQLMGAIEAVFKSWDNKRA
                     QDYRRRNKISDTLGTAVNVQAMVFGNMGGDSGTGVAFTRDPATGEDRPYGDYLENAQG
                     EDVVAGIRNTLALEEMGNVQPKAWKELQKHMKTLERHYKDMCDIEFTVEQGKLWLLQT
                     RVGKRTALAEWVIANDMIDEGLVDVGTAVRDRVTAGKLDELFKPSITASARKSESPIA
                     TGLNASPGAAVGRVVFTAADASDWAEKGEKVILVRRETNPDDYHGMVASQGILTSAGG
                     TNSHAAVVARGEGIPAVCGADSIRIDRAKKQFTAGGATVTEGDWITIDGSDGTVYVGQ
                     LDTEPSPIENAMLGNKRARKSKLWVAYERMDEVVEKTREMKVRANADTPDQAKNARAR
                     GAQGIGLCRTEHMFMGEERVAAVRHMIFADTPEEEQEAYDVLLPLQRKDFEGIFKAMD
                     GFPVTVRLLDPPLHEFLPNHVDLEV"
     gene            complement(1976..2227)
                     /locus_tag="VEV43_14320"
     CDS             complement(1976..2227)
                     /locus_tag="VEV43_14320"
                     /inference="COORDINATES: protein motif:HMM:NF018867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYP24742.1"
                     /translation="MRSKVSGKVLVHDAPANGATVELHNSSGDVVTQVMVGDDGTYAF
                     HVSEGAWSLNAWDAHGHRGSAPVTLGEGEEKNVDVVLGH"
     gene            complement(2230..3552)
                     /locus_tag="VEV43_14325"
     CDS             complement(2230..3552)
                     /locus_tag="VEV43_14325"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HYP24743.1"
                     /translation="MSGVTIDTIVNLCKRRGIVFPSSEIYGGLRSAWDWGPLGVELKR
                     NVKNEWWRSMVHERDDVVGLEASILMSPKVWEASGHVATFTDPLVECTNCHHRFRADH
                     IDLAKACPDCGTKDSWTDPRQFNLMFKTHMGPVEDSSHVVYLRPETAQGMFVDFATVQ
                     TTSRKRIPFGIAQQGRSFRNEITPGNFVFRTREFEQMEMEFFVKPGTDDEWQDYWLKE
                     RMRWYTDLGMDPEKLRLREHESDELSHYAKRTYDIEYDFPGMGWSELEGIANRTDFDL
                     KAHAEHSGQDLSYFDPDTQERYVPYVIEPAVGVERPTMAFLLDAYHEEEAPTASGKTE
                     KRTVLKLHKRLAPTKVAVLPLSKHADLMPVAEKLAAEVRPHFVTELDVTQSIGKRYRR
                     QDEVGTPFCVTVDFDSLEDRQVTVRERDSMEQERIPMEGLVEYLRKRF"
     gene            3638..4864
                     /locus_tag="VEV43_14330"
     CDS             3638..4864
                     /locus_tag="VEV43_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HYP24744.1"
                     /translation="MLAHAFFVAGEFALVAADRTRVEQLAEEGNRSAIATLHGLRTLS
                     FQLSGAQLGITITSLLVGFLAESLPMSVAVALVAATAFEMVVAELVPKNLAVARPLTV
                     AFLVVRPLIGINRLFRPLIVFLNSAANWTVRRFGIEPQEELLSVRSLEELDLLIHSSR
                     KEGALPKEEFELLAKSIEFGNKSADDALVPRVNVVALQEDDTVAQMFDVAVERGFSRF
                     PVYGKDLDDVTGIVYVKDGYAVPIDRRAATPVSEVMQEPVVVPESRPLASLLLEMRRQ
                     RRHMAVVADEYGGTAGIVTMEDLLEEIVGDIEDEYDPGGEARLTSPPEGIHLLSGLLH
                     RGEVADACGFEMPEGHYETLAGFLLDRLEHIPEGGEHVSYDGWEFKVVEMDGHRISQV
                     LCVAPPGRDDDQDVEP"
     gene            4861..6180
                     /locus_tag="VEV43_14335"
     CDS             4861..6180
                     /locus_tag="VEV43_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HYP24745.1"
                     /translation="MTGWYLLIALGLLAANGFFVGAEFAVIAARRTKIDGLAASGDVK
                     ARLAQKSIKELSFVLAGVQLGITMASLGLGAVAEPAVAHLIESALHSVVDLSEGLLHS
                     ISFVIALTIVVFFHMVIGEMAPKNVAIAEPEKSALWLALPLRIYANVFRPVIRGLNMI
                     ANVGTRLFGVEPQEERTEVHTAGELRAMIADSAREGMVDAFEHRLLSGAIGFGTRDAA
                     SIMVPRTEMVAVPATITPSELEGFVLATGHSRFPVYAGDLDNVVGFVHAKDLLKMPVG
                     GREQPLERKWIRQMLVVPESLRLHPLLVDMRRERHQFALVVDEHGGTAGIVTLEDLLE
                     ELVGEIRDEYDFSEAGVEQLSERRFLVPGSYPIYEAGERLGVHLPPGEYETVAGFIMG
                     RLGRIPRRRDVVEHEGWRLRVRAMHRRRVVQVLIEEAEPEEAPPGPR"
     gene            6191..>6724
                     /locus_tag="VEV43_14340"
     CDS             6191..>6724
                     /locus_tag="VEV43_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYP24746.1"
                     /translation="MDTKRIAAPGRAARPHIVRGVVAGAIAIAGMVVAARGQLFANPD
                     GGPDAKDVDAAEVVLAVSGAVLLLVAGVIATRSFAKAVRRAMEEHVGDQRGAALGTVV
                     TFVGYATVVLSTLGALKVDLQGLLLGGALTGVVVGIAAQQTLGNFFAGMVLLMVRPFN
                     LGDYVVLRSSPLGGEYEG"
BASE COUNT          924 a         2263 c         2332 g         1205 t
ORIGIN      
        1 cgacctcgag gtcgacgtgg ttcggcagga actcgtggag cggcgggtcg agcagcctga
       61 ccgtgacggg gaacccgtcc atcgccttga agatgccttc gaagtccttg cgctggagcg
      121 ggagcaggac gtcgtacgcc tcctgctcct cctcgggcgt gtcggcgaag atcatgtggc
      181 ggacggcggc gacgcgctcc tcgcccatga acatgtgctc ggtgcggcac agcccgatcc
      241 cctgcgcccc gcgagcgcgg gcgttcttcg cctggtccgg cgtgtcggcg ttcgcgcgga
      301 ccttcatctc gcgcgtcttc tcgacgacct cgtccatgcg ctcgtacgcg acccagagct
      361 tgctcttgcg ggcccgcttg ttgccgagca tcgcgttctc gatcggcgac ggctccgtgt
      421 cgagctggcc cacgtagacg gttccgtcgg agccgtcgat cgtgatccag tcgccttccg
      481 tcacggtcgc accacccgcg gtgaactgct tcttggcgcg gtcgatgcgg atcgagtcgg
      541 cgccgcagac tgcggggatc ccctcaccgc gtgcgacgac tgcggcatgg gagttcgttc
      601 cgccggcaga cgtcaggatg ccctgcgacg cgaccatgcc gtggtagtcg tccgggttcg
      661 tctcgcggcg gacgaggatg accttctcgc ccttctcggc ccagtccgac gcgtcagccg
      721 ccgtgaacac gacacgtccg accgcggcgc ccggcgacgc gttcaacccc gtcgcgatcg
      781 gcgactcgga cttgcgggcc gaggccgtga tgctcggctt gaagagctcg tcgagcttcc
      841 cggccgtgac gcggtcgcgg acggcggtgc cgacgtcgac gaggccctcg tcgatcatgt
      901 cgttcgcgat cacccactcc gcgagcgcgg tgcgcttgcc gacgcgggtc tgcagcagcc
      961 acagcttccc ctgctcgacc gtgaactcga tgtcgcacat gtccttgtag tggcgctcga
     1021 gcgtcttcat gtgcttctgg agctctttcc acgccttggg ctggacgttg cccatctcct
     1081 cgagcgcaag cgtgttgcgg atgccggcga cgacgtcttc gccctgtgcg ttctcgaggt
     1141 agtcgccgta gggccggtcc tcgccggtcg cggggtcgcg cgtgaacgcg acgccggtgc
     1201 cggagtcgcc gcccatgttc ccgaagacca tcgcctggac gttgaccgcg gtgccgagcg
     1261 tgtccgagat cttgttgcgc cggcggtagt cctgggccct cttgttgtcc cacgacttga
     1321 agacggcctc gatcgcgccc atgagctgct cttcgggctt gtcggggaac gcgaaccccg
     1381 ccttgcgcac gatgcccttg aacgtcttga cgagggcctt caggtcgcgc acgtctaggt
     1441 cgacgtcgtt gctcactttc ttcttgtgct tcgcctcttc gagcgcgtcc tcgaacaggt
     1501 cgccctcgat cccgagcacg gtcttgccgt acatctggac caggcggcga taggaatccc
     1561 aggcgaagcg ctcgtcaccg gtctgctgcg cgaggccggc gaccgactcg tcgttcagcc
     1621 cgacgttgag gaccgtgtcc atcatcccgg gcatcgagaa gggcgccccc gagcgaaccg
     1681 agacgaggag cgggtcgcgc tcgtcgccga gcccccgccc catcttctcc tcgaggaact
     1741 tcacgttctc gcggacctcg tcgggaaggc cgttcgggaa ctcctgaccg ccctcgcggt
     1801 aggcgttgca ggcctcggtc gtgatcgtga agccgggcgg aacggggagg ccgaggttcg
     1861 tcatctcggc gaggttcgcc cccttgcccc cgaggaggtt cttctgggac ctgtcgccct
     1921 cttcgaattg gtagacccac ttcgtcatgg tgcgaaggac cccctcgttg agaagttagt
     1981 ggccgagcac gacgtctacg ttcttctcct caccttcccc cagggtgacg ggggccgaac
     2041 cccggtggcc gtgcgcgtcc cacgcgttga gcgaccaggc cccctcgctc acgtggaagg
     2101 cgtaggtccc gtcgtcgccg accatcacct gggtgacgac gtctccggag gagttgtgga
     2161 gctctacggt cgcgccgttc gccggcgcgt cgtgcacgag taccttgccg ctcaccttgc
     2221 tgcgcatggt tagaacctct ttcgcaggta ttcgaccagg ccttccatcg gtatccgctc
     2281 ttgctccatc gagtcgcgct cgcggacggt gacctgccgg tcctctagcg agtcgaagtc
     2341 gaccgtcacg cagaaggggg ttccgacctc gtcctggcgc cgatagcgct tgccgatgct
     2401 ctgcgtgacg tcgagctcgg tgacgaagtg cggccgcacc tccgcggcca gcttctcggc
     2461 caccggcatc aggtctgcgt gcttgctgag cgggagcacc gccaccttcg tcggcgcgag
     2521 cctcttgtgc agcttcagca ccgtgcgctt ctcggtcttg cccgaagcgg tcggcgcctc
     2581 ctcctcgtgg taggcgtcga gcaggaacgc catggtcggc ctctcgacgc cgacggccgg
     2641 ctcgatcacg taggggacgt aacgctcctg cgtgtccggg tcgaagtacg agaggtcctg
     2701 gccggagtgc tcggcgtgcg ccttcaggtc gaagtcggtg cggttcgcga tgccctcgag
     2761 ctcggaccag cccatgcccg ggaagtcgta ctcgatgtcg tacgtgcgct tcgcgtagtg
     2821 ggagagctcg tcggactcgt gctcgcgcag ccgcagcttc tcggggtcca tcccgaggtc
     2881 ggtgtaccag cgcatgcgtt ccttcagcca gtagtcctgc cactcgtcgt cggtcccggg
     2941 cttgacgaag aactccatct ccatctgctc gaactcgcgc gtgcggaaga cgaagttgcc
     3001 cggcgtgatc tcgttgcgga acgaacggcc ctgctgcgcg atcccgaacg ggatgcgctt
     3061 gcgcgacgtc gtctggaccg tggcgaagtc gacgaacatc ccctgggcgg tctcgggacg
     3121 caggtagacg acgtgcgagg agtcctcgac cgggcccatg tgggtcttga acatgaggtt
     3181 gaactggcgg gggtcggtcc acgagtcctt ggtcccgcag tccgggcacg ccttggcgag
     3241 gtcgatgtgg tcggcgcgga agcggtggtg gcagttcgtg cactcgacca gcgggtccgt
     3301 gaacgtcgcg acgtggccgg acgcctccca gaccttcggc gacatcagga tcgaggcctc
     3361 gagccccacg acgtcgtccc gctcgtggac catcgagcgc caccactcgt tcttgacgtt
     3421 gcgcttgagc tcgacgccga gcggccccca gtcccacgcc gagcgcagcc cgccgtagat
     3481 ctcgctggag gggaagacga tcccccgccg cttgcacagg ttgacgatcg tgtcgatggt
     3541 cacgccgctc atgcgggcat ggtagtcggg tgaatccggc ctcccgtcgg tcgctcccat
     3601 agacggcacc tggaggctcg tcgcagtcct cgcgctgatc ctcgctcacg ccttcttcgt
     3661 cgcgggcgag ttcgccctcg tcgcggcgga ccggacccgc gtcgagcaac tggccgagga
     3721 aggcaaccgc agcgcgatcg cgacgctgca cgggctgagg acgctgtcgt tccagctgtc
     3781 gggggcccag ctcgggatca cgatcacgtc gctgctcgtc ggcttcctcg cggagtcgct
     3841 gccgatgtcg gtcgccgtcg cgctcgtcgc agcgacggcg ttcgagatgg tcgtcgccga
     3901 gctcgtcccg aagaacctcg cggtggcccg tccgctcacc gtcgcgttcc tcgtcgtccg
     3961 tcctctgatc gggatcaacc ggctgttccg gccgttgatc gtgttcctga actcggccgc
     4021 gaactggacc gtgcgtcggt tcgggatcga gccgcaggag gagctgctgt cggtacggtc
     4081 cctcgaggag ctggacctgc tcatccactc gtcgcgcaag gagggcgcgc tccctaagga
     4141 ggagttcgag ctcctcgcca agtcgatcga gttcggcaac aagtccgccg acgacgcgct
     4201 cgtgccgcgg gtgaacgtcg tcgcgctgca agaggacgac accgtcgcgc agatgttcga
     4261 cgtcgcggtc gagcgcggct tctcgcgctt tcccgtctac ggcaaggatc tcgacgacgt
     4321 cacggggatc gtctacgtga aggacgggta cgccgtcccg atcgaccgcc gcgcggcgac
     4381 gccggtgtcc gaggtcatgc aggaaccggt ggtcgtgccg gagtcgcggc cgctcgcttc
     4441 gttgctgctc gagatgcgcc gtcagcgcag gcacatggcg gtcgtcgccg acgagtacgg
     4501 gggcaccgcc gggatcgtga cgatggagga cctgctcgag gagatcgtcg gcgacatcga
     4561 ggacgagtac gaccccggcg gcgaggcacg cctgacgtct ccgcccgagg ggatccacct
     4621 cctctccggg ctgctgcacc gcggcgaggt cgccgacgcg tgcgggttcg agatgccgga
     4681 gggccactac gagacgctgg cggggttcct gctcgaccgg ctcgagcaca tccccgaggg
     4741 aggcgagcac gtctcctacg acgggtggga gttcaaggtc gtcgagatgg acggccaccg
     4801 catctcgcag gtcctgtgcg tggccccgcc gggccgggac gacgaccagg acgtggagcc
     4861 gtgaccggct ggtatctcct gatagcgctc gggctcctcg ccgcgaacgg gttcttcgtg
     4921 ggggccgagt tcgccgtcat cgcggcgcgg cgcacgaaga tcgacgggct ggccgcgagc
     4981 ggtgacgtga aggcccggct cgcccagaag tcgatcaagg agctgtcgtt cgtcctggcc
     5041 ggcgtccagc tggggatcac gatggcgtcg ctgggcctcg gcgccgtcgc cgagcccgcg
     5101 gtcgcgcacc tgatcgagag cgcgctccat tccgtcgtgg acctctctga aggtctgttg
     5161 cactcgatct cgttcgtgat cgcgctgacg atcgtcgtct tcttccacat ggtgatcggc
     5221 gagatggcgc cgaagaacgt cgcgatcgcg gagccggaga agagtgctct gtggctcgcc
     5281 ctgccgctgc ggatctacgc gaacgtcttc cgcccggtca tccgggggct caacatgatc
     5341 gcgaacgtcg gcacgcgcct gttcggcgtc gagcctcagg aggagcggac ggaggtccac
     5401 accgccggcg agctgagagc gatgatcgcg gactctgctc gcgaggggat ggtcgacgcg
     5461 ttcgagcacc gcctgctctc gggagcgatc ggcttcggga cccgcgacgc ggcttcgatc
     5521 atggtgccgc gcaccgagat ggtcgcagtg cccgcgacga tcacgccttc cgagctggag
     5581 ggcttcgtgc tcgctaccgg ccactcgcgc ttccccgtct acgcgggcga cctcgacaac
     5641 gtcgtcgggt tcgtccacgc gaaggatctc ttgaagatgc cggtcggggg ccgcgagcag
     5701 cctctggagc ggaagtggat ccgtcagatg ctcgtcgtgc ccgagtcgct gcgcctccac
     5761 ccgctgctcg tcgacatgcg ccgcgagcgt caccagttcg cgctcgtggt cgacgagcac
     5821 gggggcacgg cggggatcgt gacgctcgag gacctgctcg aggagctcgt cggcgagatc
     5881 cgagacgagt acgacttctc cgaggccggg gtcgagcagc tcagcgagcg gcgcttcctc
     5941 gtgccgggga gctatccgat ctacgaggcc ggggagcggc tcggcgtcca cctccccccg
     6001 ggcgagtacg agacggtcgc cgggttcatc atgggcaggc tcgggcgcat cccgcggcgg
     6061 agagacgtcg tcgagcacga gggctggcgg ctgcgggtgc gtgcgatgca ccgccggcgt
     6121 gtggtgcagg tgctgatcga ggaggccgag ccggaggagg ccccgccggg cccgcgttag
     6181 cctgcgcgcc atggacacga aaaggatcgc ggcgccgggc cgcgccgcga ggccgcacat
     6241 cgtccgcggg gtggtggccg gcgccatcgc catcgcgggg atggtcgtcg ccgcacgggg
     6301 ccagctcttc gccaaccccg acggcggtcc cgacgcgaag gacgtcgacg cggcggaggt
     6361 cgtgctcgcg gtctccggcg ccgtcctgct tctcgtcgca ggcgtgatcg cgacccgttc
     6421 gttcgccaag gcggtgaggc gggcgatgga agagcacgtc ggcgaccaac gcggcgcggc
     6481 gctcggcacc gtggtcacgt tcgtcggcta cgcgacggta gttctgtcga cgctcggcgc
     6541 gctgaaggtc gacctacagg gcttgctgct cgggggggcg ctgacgggtg tcgtggtcgg
     6601 gatcgcggcg cagcagacgc tcggcaactt cttcgccggg atggtgctgc tgatggtgcg
     6661 gccgttcaac ctcggcgact acgtcgtcct gcgctcgagc cccctcggcg gcgagtacga
     6721 gggc
//
LOCUS       DASQDO010000523         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1033836, whole genome shotgun sequence.
ACCESSION   DASQDO010000523 DASQDO010000000
VERSION     DASQDO010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033836"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VEV43_14345"
     CDS             <1..310
                     /locus_tag="VEV43_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24747.1"
                     /translation="DLEGFAYLARWSPGGSEVAFTVLVDADDFHTRLAVVDVETGEER
                     LLTTVDVGGIQPDWSPDGSRIAFLGFNDGGHEVGVIGRDGTNAAAITDSETDEAWLDW
                     "
     gene            423..>1833
                     /gene="glnA"
                     /locus_tag="VEV43_14350"
     CDS             423..>1833
                     /gene="glnA"
                     /locus_tag="VEV43_14350"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HYP24748.1"
                     /translation="MATSPSDVLKLGQQSGAQFVDIRFSDLPGLMQHFSMPFEEFTEE
                     AFEEGFGFDGSSIRGFQEIQESDMILVPDPETAVMDPFRTHPTMIVHAFVKDPLTGEH
                     YSRDPRYIARKAEDYLKGTGIADVAYFGPEAEFYIFDSIRFDQNQHSSFHFIGAVEGV
                     WNSGAEEEGRNLGYKPRYKEGYFPVPPMDHYQDLRSEMVLTLRDLGIPVEVHHHEVGT
                     AGQSEIDMGFGSLLDMADRLMLYKYVVKNVALRHGKTVTFMPKPIFQDNGSGMHVHQS
                     LWKDDESLMWDELGYAQLSDIARWYIGGLLEHAPALLALCAPTTNSFKRLVPGYEAPV
                     NLVYSQRNRSAAVRIPLYSKSPKSKRIEFRCPDAACNPYLAFPALLMAGLDGIKNKIE
                     PPEPVDKDLYELPPEELANVPQVPGSLDAALDALEADHDFLLQGGVFTQDVIDHWLDY
                     KRAHEVDELRLRPHPWEFYL"
BASE COUNT          320 a          605 c          614 g          294 t
ORIGIN      
        1 ggacctcgag gggtttgcct atctggccag gtggtcgccc ggcggttccg aggtcgcgtt
       61 cacggtcctc gtggacgccg acgacttcca cacgcgcctc gccgtggtcg acgtcgagac
      121 cggcgaggag cgccttctga ccaccgtcga cgtcggcggg atccagccgg actggtctcc
      181 ggacgggagc cggatcgcct tcctcggctt caacgacggg ggccacgaag tgggcgtgat
      241 cggccgcgac ggaaccaatg ccgccgctat cacggactcc gagacggacg aggcctggct
      301 cgactggtag ccgtggggtt cggcgttacg cagtcgtaac gcgacagaaa cgggcgggaa
      361 acggcgcgcg cgtacggtga cggcacgcat acgagaccgt cccgttccct taggaggagt
      421 gcatggcaac cagcccgtcc gacgtcctga agctgggtca gcagtcaggt gcccagttcg
      481 tagacatccg gttctccgac cttccgggcc tgatgcagca cttctcgatg ccgttcgagg
      541 agttcaccga ggaggcgttc gaggagggct tcggtttcga cgggtcgtcg atccgcggct
      601 tccaggagat ccaggagtcg gacatgatcc tcgtcccgga cccggagacc gcggtcatgg
      661 acccgttccg cacgcatccg acgatgatcg tccacgcgtt cgtgaaggac ccgctgaccg
      721 gcgagcacta ctcgcgtgac ccgcgctaca tcgcgcgcaa ggccgaggac tacctcaagg
      781 ggacggggat cgcggacgtc gcgtacttcg ggcccgaggc cgagttctac atcttcgact
      841 cgatccgctt cgaccagaac cagcacagct cgttccactt catcggcgcg gtcgagggcg
      901 tgtggaactc gggggccgag gaagagggcc ggaacctcgg ctacaagccg cgctacaagg
      961 agggctactt cccggtcccg ccgatggacc actaccagga cctgcggtcg gagatggtgc
     1021 tgacgctgcg cgacctcggc atccccgtcg aggtgcacca ccacgaggtc ggcaccgccg
     1081 ggcagtccga gatcgacatg ggcttcgggt cgctgctcga catggccgac cggctgatgc
     1141 tctacaagta cgtcgtcaag aacgtcgcgc tgcgccacgg caagaccgtg acgttcatgc
     1201 cgaagccgat cttccaggac aacggctcgg gcatgcacgt gcaccagtcg ctgtggaagg
     1261 acgacgagtc actgatgtgg gacgagctcg gctacgcgca gctgtcagac atcgcgcgct
     1321 ggtacatcgg gggcctcttg gagcacgcgc ccgcgttgct cgcgctgtgc gcgccgacga
     1381 cgaactcgtt caagcgcctg gtgccgggct acgaggcgcc ggtcaacctc gtgtactcgc
     1441 agaggaaccg ctcggccgcc gttcgcatcc cgctctactc gaagtcgccg aagtccaaga
     1501 ggatcgagtt ccgctgcccc gacgcggcgt gcaacccgta cctcgcgttc ccggcgctgt
     1561 tgatggcggg gctcgacggg atcaagaaca agatcgagcc gccggagccg gtcgacaagg
     1621 acctgtacga gctcccgccg gaggagcttg cgaacgtccc gcaggtcccg ggatcgctgg
     1681 acgcggccct cgacgcgctc gaggcggacc acgacttcct gctgcaaggc ggggtgttca
     1741 cgcaagacgt gatcgaccac tggctggact acaagagggc ccacgaagta gacgagctgc
     1801 gcctgcgccc ccacccgtgg gagttctacc tct
//
LOCUS       DASQDO010000524         5049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1095440, whole genome shotgun sequence.
ACCESSION   DASQDO010000524 DASQDO010000000
VERSION     DASQDO010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095440"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VEV43_14355"
     CDS             <1..262
                     /locus_tag="VEV43_14355"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HYP24749.1"
                     /translation="DRERKTHRALELKRGNVLDVAGPKQVARGLKAFVTGTSAGRPAP
                     KQDVLQLGGALVVAPGGEVLYRHQATGSEDNAPVDALLEALP"
     gene            259..420
                     /locus_tag="VEV43_14360"
     CDS             259..420
                     /locus_tag="VEV43_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24750.1"
                     /translation="MRFIPVVVILLALVAVMGGLGREGFRATGKMVLTIVALLVGFVL
                     FYLVVARAI"
     gene            complement(417..3038)
                     /locus_tag="VEV43_14365"
     CDS             complement(417..3038)
                     /locus_tag="VEV43_14365"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HYP24751.1"
                     /translation="MDAEALGTARAQAVRSASKSLAELVDEAALLRDDAPLPDRDGYA
                     SLMHAARAEGVSELRRAKRLRLLEIAARDLCGEIALEEVGRALADLADACLTASLEAN
                     DGADRLGVIAMGKLGARELNYASDIDVMFVSAGDPTEAKPLAEVLLHELGDFAPEGQA
                     FRIDTNLRPEGRSGALVRSPDGYLEYYNRWAKPWEYQALIKARHAAGAGVGAEVVERT
                     RPLVFPAEVPAERVMAVRKMKERVETHAQRSVRRGRSTESDDVKLGPGGIRDIEFSVQ
                     LLQLVHGGTDESVRSPATLDALVSLVDGGYVAEDDGAGLAVAYRWLRGVEHRLQLWQE
                     RQVHHLPRSEEERAQIARVMGFKDTPSDSALERFESYHRGVLSDVRSRFEKLFYRPMI
                     ESLADGMAARLPEEALRERLRILGFRDVERAARTLSGLVAGSSRRARLFKVLTPALLR
                     SLTATPQPDVGLFGFLTLGEALENRLDALNALRDNPPALGSLARVLGSGRLLGEVLAQ
                     VPDELSLIVAPHDEPRPKERDRLVREARASLGWREPEARWDGLRRFKRREMLRISIAD
                     VGGDVAVDEVGAGLADLADACLSAALDDLDVPLTVVGMGKLGGRELNYSSDVDVMFLH
                     DSDPAAAEKAAEQLMRAIGEVTPEGQAFRVDAALRPEGKAGPLVRSLDSYLEYYERWS
                     RPWEHLALLKARAAAGDEELGERFVAATRTYAYPAKLPPDALGEIRHLKARMEKERVP
                     RGVDPRRHLKFGPGGLADVEFSAQLLQLRHAHDLDELRVTGTVAALDACWREGLLADA
                     DALRLAEAYRFLMKARNRMFLMVGRPQDSLPSKPEELEALGVAMGYTDQPRQEMEQDY
                     LRLTRRAHKVAERVIYG"
     gene            3291..3806
                     /locus_tag="VEV43_14370"
     CDS             3291..3806
                     /locus_tag="VEV43_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYP24752.1"
                     /translation="MSAGSTRSRAYVATAITAIALSTGATTAQAHHLEDHVEQRAHVE
                     QRARTQIGAPYSYGGTSPSGFDCSGFTRWVFSQHGAALPHSSYAQFEMGKREGFKRVY
                     KREHLKEGDLVFHKTTSARVGHAGIYVGDGKFVSSTSSGGVQIRSLYDSYWGPRWVAG
                     TRVPATRRDAT"
     gene            complement(3834..>5049)
                     /locus_tag="VEV43_14375"
     CDS             complement(3834..>5049)
                     /locus_tag="VEV43_14375"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HYP24753.1"
                     /translation="AFEEGIGFDGSAIEGYARVQESDMLARPDATTFQIMRNTGGRED
                     AGARMFCDIYTPDGRPFWGDPRYVLRRNLDRAAERGFTFYVHPEMEFFVFRSPQDATP
                     IDAGGYFDLTPQGATQDLRRDTIEALERMGIPVEFSHHEVAPSQHEIDLRHADALTMA
                     DSVMTFRVAVKELAAERDMYATFMPKPRTDLPGSGMHTHMSLFEGNQNAFHDSSDEYH
                     LSKVAKAFIAGVLRHAREITAVTNQWVNSYKRLTLGPGYPVTEAPVYVCWGRHNRSAL
                     VRVPMYKPRKGQSTRIELRSPDPACNPYLAFSVILAAGMKGIDEGYDLPDEATDNIYE
                     MTDSERRARGVVQLPDDRYEAVREMESSELVAEALGEEVFAYLLRNKRAEWEQYKSYV
                     SPYEVERYLPLL"
BASE COUNT          716 a         1822 c         1741 g          770 t
ORIGIN      
        1 cgatcgggaa agaaagacgc accgggccct cgagctgaag cgcggcaacg tcctcgacgt
       61 cgccggcccc aagcaggtcg cccgcgggct caaggcgttc gtcaccggga ccagcgcggg
      121 gcgcccggcc cccaagcagg acgtcctcca gctcggaggc gcgctcgtcg tcgcccccgg
      181 cggcgaggtc ctctaccgcc atcaagccac ggggtcggag gacaatgccc ccgtggacgc
      241 tctgctcgaa gcgctgccgt gaggttcatc ccggtcgtcg tgatcctgct tgcgctcgtc
      301 gcggtgatgg gcgggctcgg ccgcgagggc ttccgggcaa cgggcaagat ggtgctcacg
      361 atcgtcgcgt tgctcgtggg cttcgtcctg ttctatctcg tcgtcgcccg cgctatctag
      421 ccgtagatca cccgctcggc caccttgtga gcgcgccgcg tcagacgcag gtagtcctgc
      481 tccatctcct gccggggctg gtcggtgtag cccatcgcca cgcccagcgc ctcgagctcc
      541 tcgggcttcg acggcaggga gtcctgcggc cgccccacca tcaggaacat ccggttgcgc
      601 gccttcatca ggaagcggta cgcctcggcg agccggagcg cgtcggcgtc cgcgaggaga
      661 ccctcgcgcc agcacgcgtc gagcgcagcg acggtccccg tcacccgaag ctcgtcgagg
      721 tcgtgcgcgt gccgtagctg caggagctgc gcggagaact cgacgtccgc gagcccgccg
      781 ggaccgaact tgaggtgccg gcgagggtcc acaccgcgcg gcaccctttc cttctccatg
      841 cgcgccttga ggtgcctgat ctctccgagc gcgtccggtg ggagcttcgc cgggtacgca
      901 tacgtacgcg tcgcggccac gaagcgctca ccgagctctt cgtcacctgc ggcggcgcgg
      961 gccttcaaca gcgcgaggtg ctcccacggc cggctccacc gctcgtagta ctcgaggtac
     1021 gaatcgagcg agcgcaccaa cgggccggcc ttgccctccg gacgcagcgc ggcgtcgacg
     1081 cggaaggcct ggccctccgg cgtgacctcg cctatcgcgc gcatcagctg ctccgccgcc
     1141 ttctcggccg cggcgggatc ggagtcgtgc aggaacatca cgtcgacgtc ggaggagtag
     1201 ttgagctcgc ggcccccgag cttccccatc cccacgacgg ttagcgggac gtcgaggtcg
     1261 tcgagcgccg ccgacaggca cgcgtccgcg aggtcggcga ggccggcccc gacctcgtcc
     1321 accgcgacgt cgcctccgac gtcggcgatc gagatccgca acatctcgcg tcgcttgaaa
     1381 cgacggagcc cgtcccaccg cgcctccggc tctcgccatc cgagcgacgc gcgcgcctcc
     1441 cgcacgagac ggtcgcgctc cttgggccgt ggctcgtcgt gcggggcgac gatcagggag
     1501 agctcgtcgg gcacctgcgc gaggacttcg ccgaggagcc tcccgctgcc gagcacgcgc
     1561 gcgagggagc cgagagcagg cgggttgtcg cgcagagcgt tgagcgcgtc gagcctgttc
     1621 tccagcgcct cgccgagcgt gaggaaaccg aacaggccga cgtcgggttg cggcgtcgcg
     1681 gtcaacgacc ggagcagcgc gggcgtgagc accttgaaca gacgtgcccg ccgtgagctg
     1741 cccgccacca gacccgacag cgtccgcgcg gcccgttcga cgtcgcggaa gccgaggatc
     1801 ctcagccgtt cgcgcagggc ctcctccggg agccgcgccg ccatcccgtc ggcgagcgac
     1861 tcgatcatcg gccggtagaa cagcttctcg aagcggctgc gcacgtccga caagacgccg
     1921 cggtgatacg actcgaagcg ttcgagcgcg ctgtccgacg gcgtgtcctt gaagcccatc
     1981 acccgggcga tctgggcgcg ctcctcttcg gaccgcggga ggtggtgcac ctggcgctcc
     2041 tgccacagct ggagccggtg ctcgacaccg cgcagccacc ggtacgcgac cgcgagcccg
     2101 gcaccgtcgt cctcggcgac gtatccgccg tcgacgagcg acacgagggc gtcgagcgtc
     2161 gccggcgacc ggacgctctc gtcggtgcct ccgtgtacga gctggagcag ctggacggag
     2221 aactcgatgt cccggatgcc gccggggccg agcttgacgt cgtcggactc ggtgctgcgc
     2281 ccgcgccgta cggacctctg cgcgtgcgtc tcgacgcgct ctttcatctt gcggacggcc
     2341 atcacgcgct ctgccggcac ctccgccggg aagacgagcg gccgcgtgcg ctcgactacc
     2401 tcggccccga cgccggcgcc cgcggcgtgg cgcgccttga tcagcgcctg gtactcccac
     2461 ggcttcgccc accggttgta gtactcgagg tagccgtcgg gcgatcgcac gagcgcgccg
     2521 ctgcggcctt cggggcgcag gttcgtgtcg atgcggaagg cctggccttc cggcgcgaag
     2581 tcaccgagct cgtgcaacag cacctccgcg agcggcttcg cctccgtggg gtcgccggcg
     2641 gacacgaaca tgacgtcgat gtccgacgcg tagttgagct cgcgggcgcc gagcttcccc
     2701 atcgcgatca cgccgagccg gtccgcgccg tcgttcgcct cgagcgacgc ggtcaggcac
     2761 gcgtcggcga gatcggccag cgcgcgcccc acctcctcga gcgcgatctc gccgcagagg
     2821 tcgcgcgccg cgatctcgag gagacgcaag cgtttcgcgc gccgtagctc ggagacaccc
     2881 tcggcgcggg cggcgtgcat cagcgatgcg tatccgtcgc gatccggaag cggcgcgtcg
     2941 tcgcgcagca gcgctgcttc gtcgaccagc tcggcgaggg atttgctcgc actgcgcacc
     3001 gcctgcgcgc gtgcggtgcc gagggcctct gcatccatga ccggggcgag cctactcgcc
     3061 gtgcgcggcc gcatcccttt ctcgcgcgtc cacaaagaac gggccgtcct tctctgaggg
     3121 cgtcggttgc gagcatccgg ctcgttcgct acggtgcctc ggaatccggg cctcaaccag
     3181 gggcccccag ggctgcgcca ttcgcgtcgc gcccttacga gcaagcgggt ctacgagcgg
     3241 cgcttcgacg cgtcccgggc ccggacgttg cgaccacagc ggaggtgggc atgagcgcgg
     3301 gctctacccg atcacgggcg tacgtggcga cggccatcac ggcgatcgcc ctctcgaccg
     3361 gcgccacgac ggcgcaggcg catcatctgg aagaccacgt cgagcagcgc gcgcacgtcg
     3421 agcagcgcgc ccggacgcag atcggcgcgc cctactccta cggaggcaca agcccgagcg
     3481 ggttcgactg ctccggcttc acgcgctggg tcttctcgca gcacggcgcg gcgctgccgc
     3541 acagctccta cgcgcagttc gagatgggca agcgcgaggg cttcaagcgc gtctacaagc
     3601 gcgagcacct gaaggagggc gacctcgtct tccacaagac gacgagcgcg cgcgtcggcc
     3661 acgcgggcat ctacgtcggc gacggcaagt tcgtctcctc gacgtcctcc ggcggcgtgc
     3721 agatccggtc gctgtacgac tcgtactggg ggccgcgctg ggtcgcgggc acgagggtcc
     3781 cggcgactcg ccgcgacgcc acgtagacgg gcgtccggcc gaggccgccc gcgctagagc
     3841 agcggcaggt agcgctcgac ctcgtacggc gagacgtacg acttgtactg ctcccactcg
     3901 gccctcttgt tgcgcaggag atacgcgaag acctcttctc cgagggcttc cgccacgagc
     3961 tccgacgact ccatctccct gaccgcctcg tagcggtcgt cgggcagctg cacgacgccg
     4021 cgggcgcggc gctccgagtc cgtcatctcg tagatgttgt cggtagcctc gtcgggcagg
     4081 tcgtagccct cgtcgatccc cttcatcccc gcggcgagga tcacggagaa cgcgaggtac
     4141 gggttgcatg ccgggtcggg cgagcgcagc tcgatgcgcg tcgactgtcc cttgcgcggc
     4201 ttgtacatcg ggacgcgcac gagcgccgag cggttgtgcc gtccccagca cacgtacacg
     4261 ggggcctcgg tgaccgggta gcccggccct agcgtcagcc gcttgtacga gttcacccac
     4321 tggttcgtga ccgcggtgat ctcacgagcg tgtcgcagca caccggcgat gaacgccttg
     4381 gccaccttcg acaggtggta ctcgtccgac gagtcgtgga acgcgttctg gttcccctcg
     4441 aacaacgaca tgtgcgtgtg catccccgac cccggcaggt ccgtgcgcgg cttcggcatg
     4501 aacgtcgcgt acatgtcgcg ctcggcggcg agctccttca cggcgactcg aaacgtcatc
     4561 acggagtcgg ccatcgtgag cgcgtcggcg tgccggaggt cgatctcgtg ctgcgacggc
     4621 gcgacctcgt ggtgcgagaa ctcgaccggt atccccatgc gttccagcgc ctcgatcgtg
     4681 tcgcgccgga ggtcctgcgt cgcgccctgc ggcgtgaggt cgaagtagcc gccggcgtcg
     4741 atcggcgtgg cgtcctgggg gctgcggaag acgaagaact ccatctcggg gtggacgtag
     4801 aacgtgaacc cacgctcggc ggcccggtcg aggttgcgcc ggagcacgta gcgcgggtcc
     4861 ccccagaacg gccgtccgtc gggcgtgtag atgtcgcaga acatgcgggc gccggcgtcc
     4921 tcgcggcccc cggtgttacg catgatctgg aacgtcgtgg cgtcggggcg ggcgagcatg
     4981 tcggactcct ggacccgggc gtagccctcg atcgccgagc cgtcgaagcc gatgccctcc
     5041 tcgaacgcc
//
LOCUS       DASQDO010000525         6569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_483010, whole genome shotgun sequence.
ACCESSION   DASQDO010000525 DASQDO010000000
VERSION     DASQDO010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483010"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VEV43_14380"
     CDS             complement(<1..955)
                     /locus_tag="VEV43_14380"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYP24754.1"
                     /translation="MAKSHRYVSIKPYLPHALAATAAVIAVPVAAVALLASMMDTPPA
                     TLAVAFLALGVAIGVFLAGSHVWSKHPDSADIAFGDLMIWGWLRRRSAEARLADNTRL
                     LGLDRSGHPEAEPVEISREEQMKVLRDLTSALESKDPYTHGHSRRVERHVLRTGAAMG
                     LTVEQIEELRKAAALHDVGKIRVPDRVLRKPGQLTIEERALVEEHVVVGAWMVSSVGD
                     ADVIAGVRHHHERWDGRGYPDGLMGVEIPLYSRIIAVADAYDAMTSTRPYRASIGRDK
                     AVDVLWAEAGIQFDPMVVEAFVAALPTRATVAGLMALFAAPS"
     gene            complement(1145..1753)
                     /gene="recR"
                     /locus_tag="VEV43_14385"
     CDS             complement(1145..1753)
                     /gene="recR"
                     /locus_tag="VEV43_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HYP24755.1"
                     /translation="MFAGPVQDLIDQLARLPGIGPKSAQRVAFHLLKATPQEARLLAD
                     AIVTVKEKVRLCTVCFNVSDQEVCEYCRDTRRDQSTICVVQEPPDIVAVERTREYRGV
                     YHVLQGAISPIEGIGPEDLRVAELLERLRADEHGVRPVKEVIVATNPNTEGSATAMYI
                     GGLIGPLGVTVTRLASGLPVGGDLEYADEVTLGRALEGRRPI"
     gene            complement(1757..2089)
                     /locus_tag="VEV43_14390"
     CDS             complement(1757..2089)
                     /locus_tag="VEV43_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HYP24756.1"
                     /translation="MGRDIQKMMKQAQKMAAEMQKAQEGLADIEVEGTAGGGAVKVTA
                     TADLNVTGVTIDPGFFEGEPDADDLEMLGDTITAAVNDALGNAREAQEKTLGPIAGGI
                     GGLGIPGL"
     gene            complement(2104..3930)
                     /gene="dnaX"
                     /locus_tag="VEV43_14395"
     CDS             complement(2104..3930)
                     /gene="dnaX"
                     /locus_tag="VEV43_14395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HYP24757.1"
                     /translation="MAYLSLYRKWRPETFDDILGQEHVSKTLANAIVEDRVAHAYLFT
                     GPRGTGKTSTARILAKALNCEQGPTPSPCGKCESCRAISDGSSLDVIEMDAASHSKVD
                     ETREILAGVPLATAGGRKKVYVIDEVHMLSAGSFNALLKTLEEPPPHVVFILATTEAH
                     KVLQTIVSRTQRFDFRRITAEVLEGLLQRVAREEKIDVDEAALAAVARHAEGSARDAL
                     SALDQLSSLGHAITSADAEALLGERGEDAQWAVFEAIASGDVGRVFTEVSGQVEAGAD
                     IRQLTLSALNHARSLLLVKTAPEADSLLDLAMQDAERLKSQAESFTPGSLLRTIDLLG
                     KAIVDMRNAPNHRLLLEVALVRAADPETDPSAGGLLGRIERLERRLGIEGAAPAPEPA
                     PRTAAPPAAKPAPTEAPRKAAPPPAAKAAPAEAPASWGEPTAPAPTPERAPEPAKAPA
                     EAPAAEAPAEPEAPAPAPVAPAAVGLGHVRDAWEATLQEVKRRSRRVAAYVTPSHPVR
                     FENDVLVVEVQSEFHKDAMTDDKNRSMLADAIHAALGIRPPLRFAARGAQPEAAPAPA
                     EETAMDDYADATAADAAQHDPVELIKKGFRAEVVEDRIGPPL"
     gene            complement(4128..4395)
                     /gene="ffs"
                     /locus_tag="VEV43_14400"
     ncRNA           complement(4128..4395)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEV43_14400"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4426..4513)
                     /locus_tag="VEV43_14405"
     tRNA            complement(4426..4513)
                     /locus_tag="VEV43_14405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4477..4479),aa:Ser,seq:gga)
     gene            complement(4533..4620)
                     /locus_tag="VEV43_14410"
     tRNA            complement(4533..4620)
                     /locus_tag="VEV43_14410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4583..4585),aa:Ser,seq:cga)
     gene            complement(4643..5101)
                     /gene="tadA"
                     /locus_tag="VEV43_14415"
     CDS             complement(4643..5101)
                     /gene="tadA"
                     /locus_tag="VEV43_14415"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HYP24758.1"
                     /translation="MTRSDEDFMRLALDEAAAAPDHDDVPIGAVVVHSGEVLAAARNE
                     RELRGDPTAHAEVLALRAAAAELGTWRLGGATVYVTLEPCPMCAGALVLARVARLVYG
                     PQDPRAGAAYSLYNVVQDPRLNHQVEVAAGVLEEECAALLTAFFQGKRHR"
     gene            complement(5098..5757)
                     /locus_tag="VEV43_14420"
     CDS             complement(5098..5757)
                     /locus_tag="VEV43_14420"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HYP24759.1"
                     /translation="MREDHILSYARPHLEDGEEIAHWVRARHPEKRRAEGFMFVTAKR
                     FIICWTGSDLEPHSVQWQEIEAWGVDDEARGGPVLFIERDHSPAVAQMPVTTNEMADT
                     VSYFVREFASRAPAPVDPPQEDLWRRRYSPRSDVNVSPEQRSIAAQARRAVVTVIGLL
                     LTVGGIAIIPLPGPWSFPVILAGLAVLASEFDWAKDVRDWARERYKAAKQMIRSRRAQ
                     D"
     gene            5936..6436
                     /locus_tag="VEV43_14425"
     CDS             5936..6436
                     /locus_tag="VEV43_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24760.1"
                     /translation="METGRRQTAVDDLYRDHGRAAFRLAYLLTGSHAQAEDLVQDAFV
                     RLLARVRHVRETGTLRSYLTRSIVNLAKNEYRRQGRLRAFVSSGRGAAPSTTGLPDVE
                     VRDELHAQLLKLPPRQRAAVVLRYCEDLGEADVADALGTSAKAVRSLVGRGLETLRSM
                     NGENDG"
BASE COUNT          914 a         2302 c         2358 g          995 t
ORIGIN      
        1 acgagggggc cgcgaacagc gccatgagcc ccgccaccgt tgcacgggtc ggcagcgcgg
       61 cgacgaacgc ctcgacgacc atcgggtcga actggatacc ggcctcggcc cacagcacgt
      121 cgacggcctt gtcgcgtccg atgctcgcgc ggtacgggcg cgtcgacgtc atcgcgtcgt
      181 acgcgtcggc caccgcgatg atccgcgagt agagcgggat ctcgacaccc atcagcccgt
      241 cggggtagcc gcgcccgtcc caccgctcgt ggtggtggcg cacgccggcg atcacgtcgg
      301 cgtcgccgac gctcgagacc atccatgccc ccacgacgac gtgctcctcg acgagcgcgc
      361 gctcttcgat cgtcagctgg cccggcttgc gcagcacgcg gtccgggacc cggatcttgc
      421 cgacgtcgtg gagcgccgcc gccttgcgga gctcttcgat ctgctcgacg gtcagcccca
      481 tcgcggcccc cgtgcggagc acgtggcgct cgacgcggcg ggagtggccg tgcgtgtagg
      541 ggtccttgga ctcgagggcc gaggtgaggt cccgcaggac cttcatctgc tcctcgcggg
      601 agatctcgac gggctcggcc tccgggtgcc ccgagcggtc gaggccgagg agccgggtgt
      661 tgtcggcgag gcgggcttcg gcggaccggc gacggagcca gccccagatc atgaggtcgc
      721 cgaaggcgat gtcggccgag tcgggatgct tcgaccacac gtgggagccg gcgaggaaga
      781 ccccgatcgc cacgccgagg gcgaggaacg ccacggcgag cgtcgccggc ggcgtgtcca
      841 tcatcgaggc gagcagcgcc acggccgcca cggggacggc gatcacggcc gccgtggcgg
      901 ccagagcgtg gggtagatag ggcttgatgc tcacgtatct gtgtgacttc gccatgtccg
      961 tccttagcta agcaacctac gtttccgcat ttgcccactc acgctgcgtc acaaacttat
     1021 agcagcacaa ggcaaatctt tccacccaaa accgggagat taggcgcacc gactcggcgc
     1081 cacgtcccca ttctccgcct cgacggcgcc gggggcaatg gccctaccgg gccccccatc
     1141 cctcttagat cggtcgccgg ccctcaaggg ctcgccccag cgtgacctcg tcggcgtact
     1201 cgaggtcgcc gcccaccggc agaccgctcg ccagcctcgt gaccgtcacg ccgagcggcc
     1261 cgatcaggcc cccgatgtac atcgcggtag cgctcccctc ggtgttgggg ttggtcgcca
     1321 cgatgacctc cttcaccggc cggacgccgt gctcgtcggc ccggagccgc tcgagcagct
     1381 cggccaccct gaggtcctcc ggcccgatcc cctcgatcgg cgagatcgcg ccctggagca
     1441 cgtggtacac gccgcggtac tcgcgcgtcc gctcgacggc gacgatgtcg ggaggctcct
     1501 ggacgacgca gatcgtcgac tggtcccggc gggtgtcccg gcagtactcg cagacctcct
     1561 ggtccgagac gttgaagcac acggtgcaga gccggacctt ctctttgacc gtcacgatgg
     1621 cgtccgccag caggcgagcc tcctgcggag tcgccttgag aaggtggaac gcgacccgct
     1681 gcgcgctctt cgggccgatg ccggggagcc gggcgagctg gtcgatcaag tcctggacgg
     1741 gaccggcgaa caccgcctag aggcccggga tgccgagccc gccgatacca ccggcgatcg
     1801 ggccgagcgt cttctcctgg gcctcgcggg cgttcccgag cgcgtcgttc acggcggccg
     1861 tgatcgtgtc gccgagcatc tcgaggtcgt cggcgtccgg ctcgccctcg aagaagccgg
     1921 ggtcgatcgt cacgcccgtg acgttgaggt cggctgtagc cgtgaccttg acggccccgc
     1981 cgcctgcggt gccctcgacc tcgatgtcgg ccaggccctc ctgggccttc tgcatctcgg
     2041 ccgccatctt ctgggcctgc ttcatcatct tctggatgtc tcgtcccatg tcgccagtct
     2101 cctctataga gggggcccga tgcggtcctc gacgacctcg gcgcggaagc ccttctttat
     2161 cagctcgacc gggtcgtgct gggccgcgtc ggccgcggtc gcgtccgcat aatcgtccat
     2221 agccgtctcc tccgcgggcg ccggagcagc ctcgggctgc gcgccgcggg cggcgaagcg
     2281 gagcgggggc cggattccaa gggccgcgtg gatcgcgtcg gcgagcatcg agcggttctt
     2341 gtcgtcggtc atcgcgtcct tgtggaactc ggactggacc tcgaccacca agacgtcgtt
     2401 ctcgaagcgg accgggtgcg acggcgtgac gtaggcggcg accctccggc tgcgccgctt
     2461 cacctcttgg agggtcgcct cccaggcgtc gcggacgtgg ccgagcccga cggcggcggg
     2521 ggcgaccgga gccggtgcgg gcgcctcggg ctctgcgggt gcttcggccg ccggagcttc
     2581 ggccggggcc ttggctggtt ccggagctcg ctcgggggtg ggagccgggg ccgtgggctc
     2641 tccccagctg gccggggcct cggcgggcgc cgccttcgcc gccgggggcg gggccgcctt
     2701 gcggggggct tcggtgggcg ccggcttcgc ggcgggcggg gctgccgtgc ggggggcggg
     2761 ctcgggggcc ggggcggctc cctcgatccc caggcggcgc tcgagccgct cgatccggcc
     2821 caggaggccg ccggccgagg ggtcggtctc ggggtcggcg gcgcgcacga gcgccacctc
     2881 gagcagcagc cggtggttcg gcgcattgcg catgtcgacg atcgccttgc cgagcaggtc
     2941 gatcgtgcgc agcagggagc cgggcgtgaa ggactcggcc tgggacttca gacgctcggc
     3001 gtcctgcatc gcgaggtcga gcagcgagtc cgcctccggc gcggtcttca cgagcagcaa
     3061 cgagcgcgcg tggttcagcg cggacagcgt cagctggcgg atgtcggccc cggcttcgac
     3121 ctggcccgag acctctgtga agacgcgtcc gacgtcgccc gacgcgatcg cctcgaacac
     3181 ggcccactgc gcgtcctcac cgcgttcgcc gagcagcgcc tctgcgtcgg cggaggtgat
     3241 cgcgtggccg aggctcgaga gctggtccag cgccgacagc gcgtcgcgtg cgctcccctc
     3301 cgcgtggcgg gcgacggccg cgagcgcggc ttcgtcgacg tcgatcttct cctcgcgggc
     3361 gacgcgctgg aggagaccct cgagcacctc ggccgtgatc cgccggaagt cgaaccgctg
     3421 agtgcgcgag acgatcgtct ggaggacctt gtgggcctcg gtcgtggcga ggatgaagac
     3481 gacgtgcggc gggggctcct cgagcgtctt cagcagcgcg ttgaacgacc ccgccgagag
     3541 catgtggacc tcgtcgatca cgtagacctt cttgcggccc ccggcggtgg cgagcgggac
     3601 gccggcgagg atctcgcggg tctcgtcgac cttggagtgc gaggccgcgt ccatctcgat
     3661 cacgtcgagc gacgagccat cggagatcgc gcgacacgac tcgcacttgc cgcacggcga
     3721 cggcgtcggg ccctgctcgc agttcaacgc cttggcgagg atgcgggcgg tgctggtctt
     3781 gccggtcccg cgggggccgg tgaagaggta cgcatgcgcg acgcggtcct cgacgatggc
     3841 gttggcgagg gtcttggaga cgtgctcctg gccgaggatg tcgtcgaacg tctcgggccg
     3901 ccacttgcgg tagagggaga gatacgccac gtcgctaacg taatgcttgg gtacgacgcg
     3961 cggccccgga tcgcgaaaaa ggatcgctag tggagggcgt tccagacgac gtccatcccg
     4021 acctagcgtc gcgccgcggg acccaatgcg gtgcctcgga gatcgtcaga caaatgtctg
     4081 acaaatctag agggccgcgc ccgcaccccc acgggaacga aaaatggccg tgcgccccgc
     4141 tctggactct ggaatggccg tcgctatccc cggcagccgg ctccggccgg gcgaccctgc
     4201 ggcactcccg ggacaccgct tacggctgct cccttccggg cctgaccggg ttcacggctc
     4261 cgagtcgcgc aggacccgac catcgacact gctttccggg ggctgcccgg tccttccaaa
     4321 gccgcaagcg agtaatcgac ccggcgtatc gagggtttcc ggttcagggg accccgagcc
     4381 ccccgcctag cacggccgag gccaggatag cccgggccgg ccgaatggcg gaggcgaagg
     4441 gatttgaacc cttggaccct ctcgggtcac acgatttcca gtcgtgccga ttcggccgct
     4501 ctcgcacgcc tccgggcggg agtctaaatc cgcggtgggg gcgggatttg aacccgcgga
     4561 gggcgtgaac cctcaatcgc tttcgaggcg atcccgttat ggccgcttcg gtaccccacc
     4621 tgggccgtcg agtatacgag cgctaccggt gcctcttccc ttggaagaac gccgtcagga
     4681 gcgccgcgca ctcctcctcg aggaccccgg ctgcaacctc gacctggtgg ttcagccgcg
     4741 ggtcctgcac gacgttgtag agcgagtacg cggcccccgc gcgcgggtcc tgcggcccgt
     4801 agacgagccg cgcgacgcgc gccaggacga gcgcgccggc gcacatgggg cacggctcga
     4861 gcgtcacgta gacggtcgcg ccgccgagcc tccaggtccc gagctccgca gccgcggccc
     4921 tcagcgcgag cacctccgcg tgcgcggtcg gatcgccccg taactcgcgc tcgttgcgcg
     4981 cggcagcgag cacctcgccg gagtggacga ctacggcgcc gatcgggacg tcgtcgtggt
     5041 cgggggcggc ggccgcctcg tccagcgcga gccgcatgaa gtcctcgtcg gagcgcgtca
     5101 atcctgggcg cgccgcgacc ggatcatctg tttggccgcc ttgtaccgct ccctggccca
     5161 gtcgcgcacg tccttcgccc agtcgaactc gctcgcgagg acggcgagcc ccgccaggat
     5221 gaccgggaaa gaccaggggc cgggcaacgg gatgatcgcg atccctccga cggtgagcag
     5281 gaggccgatc acggtcacga cggctctgcg cgcctgcgcg gcgatagacc gctgctccgg
     5341 cgacacgttc acgtccgagc gcggcgaata gcgtctgcgc cagaggtcct cctgaggagg
     5401 gtcgaccggc gccggggcgc ggctcgcgaa ctcgcggacg aagtaggaga ccgtgtcggc
     5461 catctcgttc gtcgtcaccg gcatctgtgc gaccgccggc gagtggtccc gctcgatgaa
     5521 cagcaccggg cccccgcgcg cctcgtcgtc gaccccccac gcctcgatct cctgccactg
     5581 gaccgagtgc ggctcgaggt cgctgcccgt ccagcagatg atgaagcgct tcgccgtgac
     5641 gaacatgaag ccctcggccc gccgcttctc ggggtgccgg gcccggaccc agtgcgcgat
     5701 ctcctcaccg tcctccagat ggggacgggc gtaggagagg atgtggtcct ctctcatctc
     5761 ggagatccgg cgcaaaaggc ccaagcggtg accaccctgg ggagtcggtt gctcgttctg
     5821 taggaagagt acgccgggag cggctgctac cgccgccgcg agacctctcc gggggccgcg
     5881 gtgtcctatt ggtatgacgg cggcgggttc tcgtgtgggc gcgggagggg cggcagtgga
     5941 gaccgggcgg aggcagaccg ccgtggacga cctctacagg gaccacgggc gggcggcgtt
     6001 ccggctcgcc tacctgctca ccggcagcca cgcacaggcc gaggacctcg tccaggacgc
     6061 cttcgtccgc ctgctggccc gcgtgcggca cgtccgcgag acggggaccc tgcggtccta
     6121 tctgacacgg tcgatcgtca acctggcgaa gaacgagtat cgccggcagg gccggctccg
     6181 cgccttcgtc tcgagcgggc gcggagcggc gccgtctacg acggggctcc cggacgtcga
     6241 ggtccgcgac gagctccacg cgcagctgct gaagctgccc ccgcgccaga gagccgcggt
     6301 cgtcctgcgc tactgcgagg acctgggcga ggcggacgtc gcggacgcgc tcggcacgtc
     6361 ggcgaaggcg gtgcggtcgc tcgtcggaag aggcctggag acgctgagga gcatgaacgg
     6421 agagaacgat ggatgacatc gagagaaggc tgagagagct cggcgaccgg acggaccagc
     6481 agctgcgcgc cggcgtgctc cctcgccggc gcatcgtccg gcgtgcgcgg cttcggaggg
     6541 ccgtcgtcgt tggcgcaccg gtcctcgcc
//
LOCUS       DASQDO010000526         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_238752, whole genome shotgun sequence.
ACCESSION   DASQDO010000526 DASQDO010000000
VERSION     DASQDO010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238752"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /gene="tsaD"
                     /locus_tag="VEV43_14430"
     CDS             <1..669
                     /gene="tsaD"
                     /locus_tag="VEV43_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYP24761.1"
                     /translation="EAHLYSALLVEPDATYPAVSLIISGGHTLIVHAVNHGAYEILGG
                     TLDDAAGEAFDKVARFLGLDYPGGPEIDRLSLLGNPAAIKFPRAMMNEPGFDVSYSGL
                     KTSVINYVRKQEASGEAISVEDVAASFQQAVVDVQVAKTMAAVEHAGVDRLFLVGGVA
                     ANSGLRSGMAAACEAANVKLTVPPMELCTDNAAMIAACGTAMLRRGAYSSLDVAPDPN
                     LPLA"
     gene            complement(666..1280)
                     /locus_tag="VEV43_14435"
     CDS             complement(666..1280)
                     /locus_tag="VEV43_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24762.1"
                     /translation="MDAAAPAAAGRMGIWVARVAKALFVVALVGAVWGYEYLKHYDPF
                     VHGSGSLGVSSKSDVEIDTLAGSQYLLRHEEGEKFYAWTTLHNEGRWAVTIERIKTPD
                     FGEIDVTGVFVDPTWEATGDDLQAFEATRLDSGETLQLGIEMRYGPCRIGAGDSMGID
                     SLPMRYSLLGMTRHTDLDLGYFLIVRGYQGRVCADPDNPRLDVD"
     gene            1449..1745
                     /gene="groES"
                     /locus_tag="VEV43_14440"
     CDS             1449..1745
                     /gene="groES"
                     /locus_tag="VEV43_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HYP24763.1"
                     /translation="MATNIKPLEDRILVRPEEGQETTVSGIVIPDTAKEKPQEGTVLA
                     IGPGKRSDTGEIVPMDIKVGDRVMYSKYGGTEIKVDGDELLILSSRDVLAIVGS"
     gene            1772..>2950
                     /gene="groL"
                     /locus_tag="VEV43_14445"
     CDS             1772..>2950
                     /gene="groL"
                     /locus_tag="VEV43_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180551.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYP24764.1"
                     /translation="MPKLVRFDEEARRGLEQGVNRLANAVRVTLGPRGRNVVLDKKWG
                     APTITNDGVTIAKEIELEDPWENMGAQLVKEVASKTNDVAGDGTTTATVLAQAMVKAG
                     LQSVAAGANPMGIKRGIEKAVAAAVDAIKAQSRDVEGREEIAHVASISANNDPDIGEL
                     VAEAMDKVGKDGVITVEESNTVGLELELVEGMQFDKGYISPYFVTDQDRMEAVVEDPY
                     ILIAQQKISSVRDLLPVLEKVMQSGKQLIIIAEDVEGEALATLVVNRIRGTFASVAVK
                     APGFGDRRKAMLQDIAILTGGQVISEEIGLKLENVTLDMLGRARKAVITKDATTFVEG
                     QGEESDIRGRIEQIKAEIERTDSDWDREKLQERLAKMSGGVAVLKVGAATEVELKEKK
                     H"
BASE COUNT          530 a          987 c         1015 g          418 t
ORIGIN      
        1 gaggcccacc tgtactcggc ccttctggtc gagccggacg cgacgtaccc ggcggtctcg
       61 ctgatcatct ccggcgggca cacgctgatc gtgcacgcgg tgaaccacgg tgcgtacgag
      121 atcctcggcg ggaccctcga cgacgccgcg ggcgaggcgt tcgacaaggt cgcccggttc
      181 ctcgggctcg actacccggg ggggcccgag atcgaccggc tgtcgctgct cggcaacccc
      241 gcggcgatca agttcccgcg ggcgatgatg aacgagcccg gcttcgacgt ctcgtactcc
      301 gggctcaaga cctcggtgat caactacgtc cgcaagcagg aggcctcggg cgaggcgatc
      361 tcggtcgagg acgtcgcggc gtcgttccag caggccgtgg tcgacgtcca ggtggcgaag
      421 acgatggccg cggtcgagca cgcgggcgtc gaccggctct tcttggtcgg cggggtcgcg
      481 gcgaactcgg ggctgcgctc ggggatggcg gccgcctgcg aggcggcgaa cgtgaagctc
      541 acggtcccgc cgatggagct ctgcacggac aacgcggcga tgatcgccgc ctgcgggacg
      601 gcgatgctgc gccggggggc ctactcgagc ctagacgtag ccccggaccc gaacctcccc
      661 ctggcctagt cgacgtccag tctcgggttg tcggggtcgg cacacacccg gccctggtag
      721 ccccggacga tgaggaagta tccgaggtcc aggtcagtgt gccgcgtcat cccgagtagc
      781 gagtacctca tcgggagtga gtcgatcccc atcgagtcgc ccgcgccgat ccggcagggg
      841 ccgtagcgca tctcgatccc gagctggagg gtctcgccgg aatcgagccg cgtggcctcg
      901 aacgcctgca ggtcgtctcc ggtcgcttcc caggtggggt cgacgaacac gcccgtgacg
      961 tcgatctctc cgaaatcggg cgtcttgatc cgctcgatgg tgaccgccca cctgccttcg
     1021 ttgtggaggg tcgtccacgc gtagaacttc tcaccctctt cgtgccggag gaggtactgg
     1081 ctgcccgcaa gggtgtcgat ctcgacgtcg ctcttggagg aaacccccag cgacccgctg
     1141 ccgtggacga aggggtcgta gtgcttcagg tactcgtagc cccagacggc cccgacgagg
     1201 gcgacgacga agagggcctt cgcgacccgg gcgacccaga tccccatcct gccggctgcc
     1261 gccggcgccg ccgcatccac ctggcgccga gcatagcgac gggcgggctt cgtcggcgcg
     1321 ctgcgtacgg catccgtgca ttgcgcgcca acgaccgccg tcgtgcccct gtttgacccc
     1381 ccggccccct cggcttactc ttagcactca ggcagtcaga gtgctaacac cttagtagga
     1441 ggtagcagat ggccacgaac atcaagccgc tggaggaccg catcctcgtc cggcccgagg
     1501 agggccagga gaccaccgtc tcgggcatcg tcatccccga cacggcgaag gagaagcccc
     1561 aggagggcac cgtcctcgcg atcggccccg gcaagcgcag cgacaccggc gagatcgtcc
     1621 cgatggacat caaggtcggc gaccgcgtca tgtactcgaa gtacggcggc accgagatca
     1681 aggtcgacgg cgacgagctg ctgatcctct cgtcccgtga cgtgctcgcc atcgtcggca
     1741 gctaagacca ccacagaggg gaaacgaaca catgccaaag ctggtccgat tcgacgagga
     1801 agcgcgccgc ggtctcgagc agggcgtcaa ccgcctcgcc aacgcggtgc gcgtgacgct
     1861 cggcccccgc ggccgcaacg tcgtcctcga caagaagtgg ggcgcgccga cgatcacgaa
     1921 cgacggcgtg acgattgcca aggagatcga gctcgaggac ccgtgggaga acatgggggc
     1981 ccagctcgtc aaggaagtag cgagcaagac caacgacgtc gccggtgacg gcacgacgac
     2041 cgcgaccgtc ctcgcccagg cgatggtgaa ggcggggcta cagagcgtcg ccgccggcgc
     2101 gaacccgatg ggcatcaagc gcgggatcga gaaggccgtg gccgcagccg tcgacgcgat
     2161 caaggctcag tcgcgcgacg tcgagggccg cgaggagatc gcgcacgtcg cgagcatctc
     2221 cgccaacaac gaccccgaca ttggcgagct ggtcgccgag gcgatggaca aggtcggcaa
     2281 ggacggcgtc atcacggtcg aggagagcaa caccgtcggc ctcgagctcg agctcgtcga
     2341 ggggatgcag ttcgacaagg gctacatctc gccgtacttc gtaaccgacc aggaccgcat
     2401 ggaggccgtg gtcgaggacc cgtacatcct gatcgcgcag cagaagatca gctcggtccg
     2461 cgacctgctc cccgtgctcg agaaggtcat gcagtccggc aagcagctga tcatcatcgc
     2521 cgaggacgtc gagggcgagg ccctcgcgac gctcgtcgtc aaccgcatcc gcggaacgtt
     2581 cgcctcggtc gccgtcaagg caccgggctt cggcgaccgc cgcaaggcca tgctccagga
     2641 catcgcgatc ctcacgggcg gccaggtcat ctccgaggag atcgggctca agctcgagaa
     2701 cgtgacgctg gacatgctcg gccgggcgcg caaggccgtc atcacgaagg acgccacgac
     2761 gttcgtcgag ggtcagggcg aggagtccga catccgcggc cggatcgagc agatcaaggc
     2821 cgagatcgag cggaccgact ccgactggga ccgcgagaag ctccaggagc gtctggcgaa
     2881 gatgtcgggt ggcgtggccg tcctcaaggt gggggccgcg accgaggtcg agctcaagga
     2941 gaagaagcac
//
LOCUS       DASQDO010000527         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_238892, whole genome shotgun sequence.
ACCESSION   DASQDO010000527 DASQDO010000000
VERSION     DASQDO010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238892"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VEV43_14450"
     CDS             complement(<1..733)
                     /locus_tag="VEV43_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYP24765.1"
                     /translation="MQSSDWDLPPPPVPPVTGASPWRVVIAMAIAAALIAGAFMVPIP
                     VFFLYVPGPVRDVEKLVEVDDAKTYSSEGQLFLTTVSIKTSVTFVDLISAGIDPHKDV
                     VMKEEVTGGQTLREVREQAEADMRDSKQAARQTAFAALGLPAGEGARVVETVDGSPGA
                     IALQKGDRVVAVDGEDVSTSCDVQQAIGGRAAGDVVRLTILREGERETIPIETVDHPA
                     QEGLAFVGISMADVLGDSVEGTVKTG"
     gene            complement(751..1755)
                     /locus_tag="VEV43_14455"
     CDS             complement(751..1755)
                     /locus_tag="VEV43_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYP24766.1"
                     /translation="MARDLAIDLGTANTLVYQKQKGILFAEPTVLALNKETGAVLAMG
                     DEAWKMIGRTPGYIVAVRPLRSGAISDFDITEKLLRLIMQRAGVNRLSKPKVLVCVPS
                     ALTEVERRAVEEATLSAGARTVNLIEEPMAAAIGAGLPIHEPVGNLVVDVGGGTSEVA
                     LISLGGIVTNTAVRVGGFDLDAAIQHHVRREYTVAIGERTAERIKIAIASAFPLAQEQ
                     KAELRGRDLGTGLPKNILITSEEIREAIKEPIRQIVTATLDCIGSSPPDLVQDVLTNG
                     ITLTGGGGMLAGLDMLLSQEAEVPVHVTEQPLEAVVMGAGKCLEAYQNLKGVLMGAER
                     "
BASE COUNT          253 a          673 c          679 g          296 t
ORIGIN      
        1 cacccgtctt gaccgtcccc tcgacgctgt cgccgaggac gtcggccatc gatatgccga
       61 cgaacgcgag gccctcttga gcggggtggt cgacggtctc gatcgggatc gtctcgcgct
      121 cgccctcgcg caggatcgtc aggcggacga cgtcgccggc ggcgcggccc ccgatcgcct
      181 gctggacgtc gcacgacgtc gacacgtcct caccgtccac cgcgacgacc cggtcaccct
      241 tctgcaaggc gatcgcaccg ggcgagccgt cgacggtctc gacgacgcgc gccccttcgc
      301 cggcgggaag cccgagcgcg gcgaacgcgg tctggcgcgc ggcctgcttc gagtcccgca
      361 tgtcggcctc ggcctgctcc ctcacctcgc gcagggtctg gccgcccgtc acctcctctt
      421 tcatcacgac gtccttgtgc gggtcgatgc ccgcgctgat caggtccacg aacgtgacgg
      481 acgtcttgat gctgaccgtc gtcaggaaca gctggccttc cgaggagtac gtcttcgcgt
      541 cgtcgacctc gacgagcttc tcgacgtcgc ggacggggcc ggggacgtag aggaagaaca
      601 ccgggatcgg gaccatgaac gcgcccgcga tgagagccgc ggcgatcgcc atcgcgatga
      661 cgacgcgcca cggcgacgcg cccgtcacgg gagggaccgg tggcggcggg aggtcccagt
      721 cgctgctctg catgtcctag agattgaacc tcagcgctcg gcccccatga gcacgccctt
      781 gaggttctgg tacgcctcga ggcacttgcc ggcccccata acgacggcct cgagcggctg
      841 ctcggtgacg tgcacgggga cctcggcctc ctgcgacagc agcatgtcga ggccggcgag
      901 catcccgccg ccgcccgtca gcgtgatccc gttcgtgagc acgtcctgga cgaggtcggg
      961 cggcgacgac ccgatgcagt cgagcgtcgc cgtgacgatc tgccggatgg gctccttgat
     1021 cgcctcgcgg atctcctcgg acgtgatcag gatgttcttc gggagcccgg tgccgagatc
     1081 gcgtccgcgg agctcggcct tctgctcctg cgcgagcggg aacgccgacg cgatcgcgat
     1141 cttgatccgc tccgcggtgc gctcgccgat cgcgaccgtg tactcgcggc ggacgtggtg
     1201 ctggatcgcg gcgtcgaggt cgaagccgcc gacgcgcacc gcggtgttcg tgacgatgcc
     1261 gccgagcgag atcagcgcga cctcgctcgt gcctcccccg acgtcgacga cgaggttgcc
     1321 gaccggctcg tggatcggga gcccggcccc gatagcggcg gccatcggct cttcgatgag
     1381 gttgaccgtg cgggcaccgg ccgacagcgt cgcctcctcg accgcgcgcc gctcgacctc
     1441 ggtgagcgcg ctcggcacgc acacgaggac cttgggcttc gagagccggt tcacgccggc
     1501 gcgctgcatg atcagccgca gcagcttctc ggtgatgtcg aagtcggaga tcgcgcccga
     1561 gcgcagcggc cggacggcga cgatgtagcc gggtgtccgg ccgatcatct tccaggcctc
     1621 gtcccccatc gcgaggacgg cgccggtctc cttgttgagc gcgaggacgg tcggctcggc
     1681 gaacaggatg cccttctgct tctggtagac gagcgtgttc gcggtgccca ggtcgatcgc
     1741 gaggtcgcgg gccacggcgg ggtctagaag gagcggctgc gggccgcggc gcggcgacgc
     1801 gcctcgatgc gcttgcgggc ggcggcccgg cgctgtgggt cgagcccgcc ccgggaggtg
     1861 cggtgggcgg gggcacccct gcggcccggc gaggtcgcgg c
//
LOCUS       DASQDO010000528         6905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_667347, whole genome shotgun sequence.
ACCESSION   DASQDO010000528 DASQDO010000000
VERSION     DASQDO010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667347"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VEV43_14460"
     CDS             <1..701
                     /locus_tag="VEV43_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYP24767.1"
                     /translation="QARRKAHGNRSLERRGGGHEPPHLADDVTKELRALARPGKGDLL
                     VKVFADAAGAYLDDDLDEAIRLGEQAKHMALRAPTPRELLGLAYYRAGRWREAARELS
                     AFRRLLGAPDQNPTLADCYRALDKPERAVELCDEVTPQNAGPETYYEANIVAAGALAD
                     QGRLDDAIARLEALELQPENAEEHHVRAWYALADLLERRGRFTQAKEWFEAVAAADPD
                     ATDAPQRIRRMHLG"
     gene            910..1497
                     /locus_tag="VEV43_14465"
     CDS             910..1497
                     /locus_tag="VEV43_14465"
                     /inference="COORDINATES: protein motif:HMM:NF025108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HYP24768.1"
                     /translation="MNKTRRAALVLLSSVVALATTASVARADDTNVTVTGGDLTLGSI
                     TTGDFGGITLNGAAQSTTATMNAFSVTDARGTGLGWNVTISASPFNEYASGEYVASGK
                     TLGTSRFTMAQASAAKVDASSSGVPTVTNGTYTLDADGNSDGTSDAVKIASAATGDGM
                     GSYTVTPGQLSLSVPANVYAKTYRSDVTVTLSTGP"
     gene            1560..2672
                     /locus_tag="VEV43_14470"
     CDS             1560..2672
                     /locus_tag="VEV43_14470"
                     /inference="COORDINATES: protein motif:HMM:NF017810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HYP24769.1"
                     /translation="MRSLSKVLTIAGVALVASAALPASASTAMRFTVRPDAGGSLSPG
                     GDYFVLEAAPGDVVRQALELANPGGRTVVVRLAAVDATTAQFGGVDYSPSEARPSAVG
                     KWIELGAESVTLAPGAVREVPFSVSVPAGAASGVNLGGIAAWTPPRDGGSADAQGLNA
                     TAEVQTRRVVAVQVDLPGPAEPALEIDGVRTIARPDGVYLQVDLRNTGNGFAQGEGTI
                     ELAGEGFSTDFALDKVVPGTGVGYPVRWRTKAPASGAYPVVVTIDYGADVAEYRGDVV
                     VGGGLGAELADRGIGDAGGDGFPVLPAAVAGAVCCAAAALVWRRRRGGASGAASETPS
                     EPAPVAASPPPPPPPASRNVTPPPPPPPPPVHAARG"
     gene            complement(2692..3171)
                     /locus_tag="VEV43_14475"
     CDS             complement(2692..3171)
                     /locus_tag="VEV43_14475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006663994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYP24770.1"
                     /translation="MRVVELHGPDELRRAWPVMSELRGHLSEDRWLELTEAMGPDGYR
                     LLALEDDGGTIQALAGIRVSTNLYYGRHMWVDELVTASTARSSGHGRALLGHVEELAV
                     SEGCEMVGLSSGIKRVDAHRFYEKHMGYDKASFTFTKHVGPVRDAMPPPNPEPMPGR"
     gene            complement(3168..4112)
                     /locus_tag="VEV43_14480"
     CDS             complement(3168..4112)
                     /locus_tag="VEV43_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP24771.1"
                     /translation="MAAAIIPATYIQEEPTDPITIDLDMHGFPGITGAFVVRGERVAL
                     IETGPKSCLDHLLTGLAGAGIDRVDFVVVTHIHLDHAGAAGTLAARWPDAQVVVHPVG
                     APHLVDPSKLWSSASRIYGDAMETLWGGIDPVPADRIVEVADGDSIDLGGATLRVIET
                     PGHARHHHAYLDESSGTLFSGDALGVRLPGVGVIRPATPPPEFDLEAAIASIGKIRDA
                     APERLCFTHFGLAAGEGPAETCDAAADALVEWAGWIRAARESSTDVDEVAAVVRRESL
                     AAYGASLDDDSIARLEQTTSYRMNTWGYMRYLDKLERS"
     gene            4096..5025
                     /locus_tag="VEV43_14485"
     CDS             4096..5025
                     /locus_tag="VEV43_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYP24772.1"
                     /translation="MIAAATRRSGVLIVNPGAGRASAERRHEVVDEVRRRLRVDVYET
                     PSREAGIEMAAQAADAGVTAVLAFGGDGLVNEVANGVAGTATALAILPGGTMNVFARA
                     LGIPTDPLAALDALLGRWDDGPRLVPLGRIGDRYFTFSAGCGFDAEAAARVERDYRSK
                     RAFGELFFYWSAFRVLAGSYRHRNPSMKIEGRFGEVPVAMAIGCNAGPYAYLAGRAVN
                     LAPEVALDGGLDVFALRSMRIEALPLYAWRAVVSGDLVHHRDTFYESGLDGFEITAEE
                     PFARHVDGEPLEPATSARFEVVPGVLKVLGAAR"
     gene            complement(5608..6324)
                     /locus_tag="VEV43_14490"
     CDS             complement(5608..6324)
                     /locus_tag="VEV43_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP24773.1"
                     /translation="MPLLEVEELKAGYGSLPVLQGITFDIEEGETAVLLGLNGAGKTT
                     TVLTLSGMLKPWAGRVTFQGKDVTGMDPRKLVADGVVLVPEGRHVFPQLTVAQNLRLG
                     AWPYKKDRKNTRKVVERAVEYFPRLSERWGQLAGTLSGGEQQMLAIARGLMARPKLLL
                     IDEASLGLSPKLTQTVFDVVRQINQDGTTVLIVEQNAGVLRIASRAFVMEKGTLVYRG
                     TGEDILDHSEIRQAYLGAPA"
     gene            complement(6311..>6905)
                     /locus_tag="VEV43_14495"
     CDS             complement(6311..>6905)
                     /locus_tag="VEV43_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP24774.1"
                     /translation="LPTHRRVALGLGRTFQNIGLVRELTVLENFLVAQHPRIAYGALG
                     AVLGAPRSFSEERRLRTNALEVLDFLDLTHLKDSKLDGLPYGTLKLIEIGTVLATDPD
                     VVLLDEPSSGMGPDEAHTFGERLLELRRTLNLTVLMIEHHVPLVTGVCDYVYVLNFGR
                     ILTEGKPQAVASHPEVVAAYLGTEVEETTEEPLDATT"
BASE COUNT          997 a         2426 c         2507 g          975 t
ORIGIN      
        1 accaggcgcg gcgaaaggcg cacgggaacc gctcgctcga gcgtcgcgga ggcggccacg
       61 agccgcccca cctcgccgac gacgtcacga aggagctccg cgcgctcgcc cggccgggca
      121 agggcgacct gctggtgaag gtcttcgcgg acgccgccgg cgcgtacctc gacgacgacc
      181 tcgacgaggc gatcaggctg ggcgagcaag cgaagcacat ggcgttgcgg gcgccgaccc
      241 cgcgcgagct cctgggcctc gcctactaca gggcgggtcg ctggcgcgag gccgcccgcg
      301 agctgtcggc gttccggcgg ctgctgggcg cacccgacca gaacccgacg ctggccgact
      361 gctacagggc cctcgacaag cccgagcgcg ccgtggagct gtgcgacgag gtgacgccgc
      421 agaacgcggg gccggagacg tactacgagg cgaacatcgt cgccgccggt gcgcttgcgg
      481 accagggccg gctcgacgat gcgatcgcgc ggctggaggc cctcgagctc cagcccgaga
      541 acgcggagga gcaccacgtc agggcctggt acgcgctggc ggacctgctc gagcggcgcg
      601 gccgcttcac gcaggcgaag gagtggttcg aggcggtcgc cgcggcggat cccgacgcga
      661 ccgacgctcc gcagcgcatc cggcgcatgc acctcggctg aggctcgcct tcgcagactg
      721 tctagttaca tccaatccgc acaccgggac aaatcgggcg cggggagggc cggatttcgg
      781 ccccccccgg ctatacagca attctgtcca cgggccgatg ggatgttaag tcttccggaa
      841 gacgacggcc ggcttcccag tgggggggac ggaaaagtca gtcgatcgca ccacaagaag
      901 ggaaaagcca tgaacaagac tcgcagggcc gcactcgtgc tcctttcgtc ggtagtcgct
      961 ctcgcgacca cggcgtcggt ggctcgagcg gacgatacga acgtaacggt gacgggaggt
     1021 gacctcaccc tcgggtcgat cacgaccgga gacttcgggg ggatcacgct gaacggcgcc
     1081 gcgcagagca cgaccgcgac gatgaacgcg ttcagcgtca ccgacgcccg ggggaccggg
     1141 ctcggctgga acgtcacgat ctcggccagc ccgttcaacg agtacgcgag cggcgagtac
     1201 gtcgccagcg gaaagacgct cggcacgagc cgcttcacga tggcgcaggc ttccgcagcg
     1261 aaggtcgacg ccagcagctc gggcgtcccg accgtcacga acgggacgta cacgctcgac
     1321 gccgacggca actccgacgg cacgtccgac gccgtcaaga tcgcaagcgc ggcgaccggt
     1381 gacgggatgg gcagctacac cgtcacgccg ggccagctca gcctcagcgt cccggcgaac
     1441 gtctacgcca agacctaccg cagcgacgtc accgtcacgc tctcgaccgg cccgtaggaa
     1501 cacccgaccc aagagggcgg gggctctagc agcccccgcc ctcgaaagga ttacggacga
     1561 tgcgctctct ctcgaaggtc ctcacgatcg caggggtagc tctggtcgcc tctgcggctc
     1621 tgcccgcttc cgcttcgacg gcgatgaggt tcacggtccg gcccgacgct ggcggatcgc
     1681 tgtcgccggg cggggactac ttcgtcctcg aagcggcgcc gggcgacgtc gtgaggcagg
     1741 cgctggagct cgcgaacccg ggcggccgga cggtcgtcgt tcgcctggcc gcggtagacg
     1801 cgacgaccgc acagttcggc ggcgtcgact actcgccgtc ggaggcccgc ccgagcgccg
     1861 tcgggaagtg gatcgagctg ggggccgagt cggtgacgct cgctccgggc gcggtccggg
     1921 aggtgccgtt cagcgtctcc gtccccgccg gtgcggcgtc gggcgtgaac ctcggaggca
     1981 tcgccgcatg gacgccgcca cgagacgggg gatccgcaga cgcgcagggt ctcaacgcga
     2041 ccgccgaggt gcagacacgg cgtgtcgtcg ccgttcaggt ggacctgccg ggcccggccg
     2101 agccggccct cgagatcgac ggagtccgca ccatcgcccg ccccgacggc gtctacctgc
     2161 aggtcgacct gcgcaacacg ggtaacggct tcgcccaggg agagggcacg atcgagctcg
     2221 ccggcgaggg tttctcgacc gacttcgcgc tcgacaaggt cgtgccgggg acgggtgtcg
     2281 gctatcccgt ccggtggcgc accaaggccc cggcctcggg cgcgtatccg gtcgtcgtca
     2341 cgatcgacta cggcgcagac gtcgccgagt accgcgggga tgtcgtcgtc ggcggcggcc
     2401 tcggcgcaga gcttgccgac cgcggcatcg gcgacgcggg gggcgacggt ttccctgtgc
     2461 tgcccgccgc cgtggccggc gcagtgtgtt gcgccgccgc ggccctcgtg tggcgcaggc
     2521 gcaggggcgg cgcctccggg gccgcctccg agaccccttc tgagccggct ccggtcgcgg
     2581 cgtcaccgcc gccacctccg ccgccggctt cgaggaacgt gacgccgcct ccgccacccc
     2641 ctccgccgcc ggtccacgca gcgcgcggct gatactcggg cgcggtcgtc gctaacggcc
     2701 gggcatcggc tccgggttcg ggggcggcat cgcatcccgc accggcccca cgtgcttggt
     2761 gaacgtgaac gacgccttgt cgtaacccat gtgcttctcg tagaaccggt gcgcgtcgac
     2821 ccgcttgatg cccgacgaga gcccgaccat ctcgcaccct tcggagaccg cgagctcctc
     2881 gacgtgaccc agcagcgccc ggccgtggcc cgacgagcgc gcggtcgaag ccgtgacgag
     2941 ctcgtccacc cacatgtgcc tgccgtagta gaggttcgtc gacacgcgga tcccggcgag
     3001 ggcctggatg gtgccgccgt cgtcctcgag cgcgagcaac ctgtagccgt cgggccccat
     3061 cgcttccgtg agctccaacc accgatcctc ggagaggtgc ccgcgcagct cactcatcac
     3121 gggccacgcc cggcggagct cgtcggggcc gtggagctct acgaccctca cgaccgctcc
     3181 aacttgtcga gatagcgcat gtagccccac gtgttcatcc ggtacgatgt cgtctgctcg
     3241 agccgtgcga tcgagtcgtc gtcgaggctc gccccgtacg ccgcgagcga ctcccggcgg
     3301 acgacggccg ccacctcgtc gacgtcggta gaggactcgc gggccgcgcg tatccagccg
     3361 gcccactcca cgagagcgtc cgccgccgcg tcgcatgtct ccgcgggacc ctcgccggcg
     3421 gcgagcccga agtgcgtgaa gcagagccgc tcgggcgcgg cgtcgcggat cttccctatc
     3481 gacgcgatgg cggcttcgag gtcgaactcc ggcggcggcg tcgccggacg gatcacgccg
     3541 accccgggaa gccggacgcc cagcgcgtct ccggagaaga gcgtgcccga cgactcgtcg
     3601 agataggcgt ggtggtggcg ggcatggccg ggcgtctcga tgacccgcaa ggtcgcgccg
     3661 ccgaggtcga tcgagtcgcc gtcggcgacc tcgacgatcc ggtctgccgg cacggggtcg
     3721 atccctcccc acagcgtctc catcgcgtcg ccgtagatcc gcgacgcgct cgaccacagc
     3781 ttgctcgggt cgacgaggtg cggggccccc acggggtgca cgaccacctg cgcgtccggc
     3841 cagcgcgccg cgagcgtccc ggcggcgcct gcgtggtcga ggtgtatgtg cgtgaccacg
     3901 acgaagtcga cccgatcgat cccggcgccg gcgagccccg tcagcagatg gtcgaggcac
     3961 gacttcggac ccgtctcgat caaggcgacc cgctcgccgc gcacgacgaa cgcccccgtg
     4021 atcccgggga agccgtgcat gtccaggtcg atcgtgatgg ggtccgtggg ctcctcctgg
     4081 atataggttg ccgggatgat agccgccgcc acgcggcgtt ccggtgtgct gatcgtgaac
     4141 cccggcgcgg gccgcgcgtc cgcggagcgg cggcacgagg tcgtcgacga ggtgcggcgg
     4201 aggctgcggg tcgacgtcta cgagacgccg tcgcgcgagg ccggcatcga gatggccgcg
     4261 caggccgccg acgccggagt gaccgcggtc ctggcgttcg gcggggacgg gctcgtcaac
     4321 gaggtagcca acggcgtcgc ggggaccgcg accgcgctcg cgatcctgcc cggcgggacg
     4381 atgaacgtct tcgcgcgggc actggggatc ccgaccgacc ccctcgccgc gctcgacgcg
     4441 ttgctcggcc gctgggacga cgggccgcgc ctcgtgcccc tgggccggat cggcgaccgc
     4501 tacttcacgt tctcggcggg gtgcgggttc gacgccgagg cggcggcccg ggtcgagcgt
     4561 gactaccgga gcaagcgcgc gttcggcgag ctcttcttct actggagcgc gttccgcgtg
     4621 ctcgccggca gctaccggca ccgcaacccc tcgatgaaga tcgagggacg gttcggcgag
     4681 gtcccggtcg cgatggcgat cgggtgcaac gcgggcccgt acgcgtacct cgcgggccgt
     4741 gcggtgaacc tcgcccccga ggtggcgctc gacggcggcc tggacgtgtt cgcgctcagg
     4801 agcatgagga tcgaggccct gccgctctac gcgtggcgcg cggtcgtctc gggcgacctc
     4861 gtccaccacc gcgacacgtt ctacgagtcc ggcctggacg ggttcgagat caccgcggag
     4921 gagccgttcg ctcgccacgt cgacggggag cccctggagc ccgccacgtc cgcgcgcttc
     4981 gaggtcgtcc ccggcgtcct gaaggtgctg ggggccgcgc ggtgagcgtg ctcttgacgg
     5041 ggctggagcc ggagctggtc gaggcgatca cccgccggct cgccgcggcg ggcgaccagg
     5101 tgagggtcgt agcggcggcg ggtcagacct acgagggcgc gcacgtcgcg gccggcgacc
     5161 ccggcgacga cgacctcgtc gagcgcgctg cacagggcgc ccgcacgatc gtcgcggggc
     5221 cggagggccg agccggtgcg ctcgagggag ccgcccgcgc gggcgtcgac cgcgccgtcc
     5281 tgctgggcgg cgacgcgggc gacgtgccgg cctcgatgtc gtgggtgctc ctcgtgacga
     5341 agcggcggct ccggcggggg gtgggggtgg acgacgtggc cgaagcggtg gacgcggccg
     5401 acgacatggc cggcgagccg cggctgacgg ccgacctgac gaccgaagaa ggctggaagg
     5461 ccctgcgcct cgacccgcgt tgccgggtgt tcttcccgag ggtgtggttc acgtggttgc
     5521 gaaggttggt gaggttggga gtgcaacctc gccaaccacg cgaaccacgt gtaccagagc
     5581 gtccggacgg acaggtgtcg ctcggcccta ggcgggcgct cctaagtacg cttggcggat
     5641 ctccgagtgg tcgaggatgt cctcgccggt gccccggtag accagcgtcc ccttctccat
     5701 cacgaaggct cgcgacgcta tgcgcaggac tcccgcgttc tgctcgacga tcagcaccgt
     5761 cgtcccgtcc tggttgatct ggcgcacgac gtcgaacacg gtctgggtca gcttcggcga
     5821 cagccccagc gaggcctcgt cgatcagcag gagcttcggc ctcgccatga ggccacgtgc
     5881 gatcgcgagc atctgctgct cgccgccaga cagcgtcccc gccagctgtc cccagcgctc
     5941 gctcagccgc gggaagtact cgaccgcgcg ctcgacgacc ttgcgcgtgt tcttgcggtc
     6001 cttcttgtac ggccacgcac cgaggcggag gttctgcgcg accgtgagct gcgggaacac
     6061 gtggcgaccc tcggggacga ggacgacgcc gtcggctacg agcttgcgcg ggtccatgcc
     6121 cgtgacgtcc ttgccctgga aggtgacgcg gcccgcccag ggcttcagca tccccgagag
     6181 cgtcaggacc gtcgtggtct tgcccgcgcc gttgaggccg aggaggacgg cggtctcccc
     6241 ctcttcgatg tcgaacgtga tcccctgcag gacgggtagc gagccgtacc ccgccttcag
     6301 ctcttcgacc tcaagtagtg gcatcgagcg gctcctcggt ggtctcttcg acttcggtcc
     6361 cgaggtacgc ggcgacgacc tcggggtgcg acgcgaccgc ctgcggcttg ccctcggtca
     6421 ggatccgccc gaagttcagc acgtagacgt agtcgcagac gccggtgacg agcgggacgt
     6481 ggtgctcgat catcagcacg gtgaggttga gtgtcctgcg gagctcgagc aggcgctcgc
     6541 cgaacgtgtg cgcctcgtcc gggcccatcc ccgacgacgg ctcgtcgagc agcacgacgt
     6601 cggggtcggt cgcgaggacc gtgccgatct cgatgagctt gagggtcccg taggggagcc
     6661 cgtcgagctt cgagtccttc aggtgggtga ggtcgaggaa gtcgaggacc tcgagagcgt
     6721 tcgtgcgcag ccggcgctcc tccgagaacg acctcggcgc accgaggacc gcgccgaggg
     6781 ccccgtaggc gatgcgcggg tgctgcgcga cgaggaagtt ctcgagcacg gtgagctcgc
     6841 gcacgaggcc gatgttctgg aacgtgcgcc cgagcccgag cgcgacgcgg cggtgggtcg
     6901 gcagc
//
LOCUS       DASQDO010000529         8317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_543877, whole genome shotgun sequence.
ACCESSION   DASQDO010000529 DASQDO010000000
VERSION     DASQDO010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543877"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1927)
                     /locus_tag="VEV43_14500"
     CDS             complement(71..1927)
                     /locus_tag="VEV43_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP24775.1"
                     /translation="MRSNASARVSVTIALAAALLVALLPASAGAAAKRGRILPAGKAV
                     PGSYIVMLESSVPSVPDVATSLARIHDGELGWVYNHAIKGFSIEIDRAGALAISADPR
                     VKLVEEDPVVRAYATQSNPPYGLDRIDQRNLPLSNSYVYNATGSGTTAYVIDSGVRIT
                     HSDFGGRASHGYDFVDGDPDASDCDGHGTHVAGTVGGTTYGVAKSASLVAVRVLDCNG
                     DGVGSDIIAGVDWVTAQHTAGEKDVANMSLGTQIGTSSTIDAAVRNSIADGTVYALAA
                     GNGGPLGVADNACNYSPSRVTEAIVVSATDSQDRKASFANYGTCVDIFAPGVSVVSTW
                     LTNDTSTNSISGTSMASPHVAGAAAQYLQANGATTPPAVESALNTNATTGKVVSPGSG
                     SPNRLLYNAFISAGTTPTPTPTPPPGNAAPTASYTHSCSGLTCTFTDTSTDSDGTIAS
                     RSWNFGDGGTSTAANPSHTFPAAGTYTVALTVTDNGGATGNTSKGVAVTSSSDPDPST
                     PTLSNGATVSDTNGAAGTWKYYKIQVPAGRPSLKVDLTGAACGLISCDPDLDLYVRQG
                     AKPTETAYACRHYTSASTGSCTVSSPAGDWWYIGVHVYAGSTSKSYSIKATY"
     gene            2151..2636
                     /locus_tag="VEV43_14505"
     CDS             2151..2636
                     /locus_tag="VEV43_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HYP24776.1"
                     /translation="MGEIQEVKLPGVGVRYEFETAEGNRIGVVSHRSGLREVYGAKRE
                     DPDEFKRLLGLSPDDARTLAELLGATRVAEQLAELQQRIEGLAIDWLPVREDSTYAGR
                     PIGDTKMRTRTGVSVVAIVRGDQAVPAPGPEVHVESGDYLVVVGTARGIEEAVALLRA
                     G"
     gene            2642..3796
                     /locus_tag="VEV43_14510"
     CDS             2642..3796
                     /locus_tag="VEV43_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYP24777.1"
                     /translation="MEHPELLVELGAVLLGLAVLSRLAVRTGMPTIPLYLAAGLAFGE
                     GGIVPIVTADEFVQVGAELGLILLLFMLGLEYTATELLTTIRSQARVGVLDFVLNFPP
                     GFFAALILGWGPTLGVVLGGVTYVSSSGIVAKILSDQGWTGNRETPVVLSTLVIEDLV
                     MALYLPVAGALLAGSGGIASLLPAFGAIAVVAVVLAAATRVEVGISRVLFSRSDEALL
                     LTILGITILVAGGAELFGVSAAVAALLVGIVLSGPAAEAAHGLLTPMRDLFSALFFAF
                     VGLAVDPATIPPVLLPAAALAVVTFATKALTGWMGAARAGVGVRGATRAGLLLAARGE
                     FSIAIAGFATAAGVAGDFEALAVAYVFLLAIAGPVLVRFADAISAPLVRP"
     gene            3927..4058
                     /locus_tag="VEV43_14515"
     CDS             3927..4058
                     /locus_tag="VEV43_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24778.1"
                     /translation="MSVKPTFCSTCNRSVYVGHTDSLSCPVCSTPLLETVGVEEDAR"
     gene            4055..4432
                     /locus_tag="VEV43_14520"
     CDS             4055..4432
                     /locus_tag="VEV43_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYP24779.1"
                     /translation="MSPTVLVVDDDEEVRDALRLLFELDGFSVVGEAAHGVDGVALAH
                     RHQPDFVILDSVMPYLGGAGTASILRAIAPSSKIVAFSAALDSRPAWADAFLNKDRVG
                     EIAPFVTAMIPARPRREPAGTLV"
     gene            4432..4833
                     /locus_tag="VEV43_14525"
     CDS             4432..4833
                     /locus_tag="VEV43_14525"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HYP24780.1"
                     /translation="MQQVRPGTVPVVLYDADCGFCTWVVDRIAARVAPGALEIVPLQS
                     TRADDLLGGRVDEAAKWESWHLVEADGSLHSGGEAVPLVLRHVRGGRAMGRVAARFPR
                     ATAGAYRLVARNRDRLGRVVRADRCRVPGAD"
     gene            4894..5358
                     /locus_tag="VEV43_14530"
     CDS             4894..5358
                     /locus_tag="VEV43_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24781.1"
                     /translation="MQKIVPHLWFDTEAEDAAKFYTSLFPNSSITDVSHYGEAGPRPA
                     GTVLTVSFELDGQEFTALNGGPEFKFNEAVSFLVNCETQEEVDDLWDRILESGGEESQ
                     CGWIKDGFGLWWQIVPTALPRLLQGEDSDRANRVMQAMFQMRKLDIATLESA"
     gene            5423..5752
                     /locus_tag="VEV43_14535"
     CDS             5423..5752
                     /locus_tag="VEV43_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24782.1"
                     /translation="MRFVAALIALSSFVACGGDAAGSEAFCDATRKIIELGNVEEVPP
                     EVDTMVEEAPDEIKDATETVRDVFVQAFEDRDLSQLQSQEFQDAATELREYAVENCDN
                     VRDITNE"
     gene            5846..6361
                     /locus_tag="VEV43_14540"
     CDS             5846..6361
                     /locus_tag="VEV43_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYP24783.1"
                     /translation="MKRGDVIAYETLVQRHQSAAFRTAYLVAGSANEAEDAAQEGFLK
                     AFYAIDRFRSDAPFRPWLLAIVANEAKTRLRSASRRQTLALRLTEGRLEGGAVPSPEE
                     AALARSEKERVLEALGHLGEDDRLVIGYRYFLELSEKETAAALGVRRGTVKSRLSRAL
                     VRLRSVMEEGS"
     gene            6358..7149
                     /locus_tag="VEV43_14545"
     CDS             6358..7149
                     /locus_tag="VEV43_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24784.1"
                     /translation="MTRRRDPALAALLQEAARGTAFPETPALAESVRERIERGPMPVA
                     EVRLPRTRPPLLRPVLVTLLAIAVALGVTLSISVTARRAVADLLGVVGIRITFGDDPP
                     GSPTPARRIRLGDAVPRKTASELAGFRVLVPGAAAGRPAIYFDRRVGDRGMVTVVYPR
                     AAETLAHVDLLVSEFVASLPAEYVKKLGTLGSRITYTDVRGVPAYWVSGDPHYFFFTE
                     RNGAVRQETIRLAGNVLLWEEDGVTFRVEGAGSLREARRLAESLR"
     gene            7213..7794
                     /locus_tag="VEV43_14550"
     CDS             7213..7794
                     /locus_tag="VEV43_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP24785.1"
                     /translation="MTRRVPLLLAVLLGGAVAFAPGVAAGGFCHADPAARMKTSSAPR
                     VTMIDCAFETTVTYVDPGEKVRWVNKDRSPHTVTGAALSWGTEEMLDMGDAVTYSFEE
                     EGVFPYYCELHPAMVGAVVVGDGGGPAGAAGTGVKPVDDAASVAATETAPAGASDGLS
                     PAVLALALAAAAGGLVVVTRAVWARRAGAASAS"
     gene            7868..8251
                     /locus_tag="VEV43_14555"
     CDS             7868..8251
                     /locus_tag="VEV43_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24786.1"
                     /translation="MANPVVHFEAIGPDGPALIGFYGKAFGWSINADNPMGYGMVDNG
                     GEGINGGIGQAPPEGNGHHLTWYVWVEDIDAALETIENLGGKTINPKMTVPGGPTVAH
                     FEDPAGNFVGLVTGGPEEMGTAAGS"
BASE COUNT         1163 a         2823 c         3009 g         1322 t
ORIGIN      
        1 cgaaggcggc ccccgtcccg ttcttgctgg ccgatcgccg aggcgatcgg tcgtccctgc
       61 ccctctggac ctagtaagtc gctttgatgc tgtagctctt cgacgtcgaa cccgcgtaga
      121 cgtggacgcc gatgtaccac cagtcgccgg ccgggctcga gaccgtgcac gaccccgtcg
      181 aagccgaggt gtagtgcctg cacgcgtatg cggtctcggt cggcttcgcg ccctgacgga
      241 cgtagaggtc gaggtccggg tcgcagctga tcagtccaca cgccgcgccg gtcaggtcga
      301 ccttcagcga cggccggccc gccgggacct ggatcttgta gtacttccac gtgccggcgg
      361 cgccgttcgt gtcggacacc gtggcgccgt tcgagagcgt cggcgtgctc gggtccgggt
      421 cgctcgacga ggtgacggca acacccttcg acgtgttgcc ggtggcaccg ccgttgtccg
      481 tcaccgtcag cgcgaccgtg taggtgccgg ccgccgggaa cgtgtggctc gggttcgcgg
      541 cagtcgacgt gccgccgtcg ccgaagttcc agctacggct cgcaatcgtg ccgtcggagt
      601 ccgtgctcgt gtccgtgaac gtgcacgtga ggcccgagca cgagtgcgtg tacgaagccg
      661 tcggagccgc gttgcccgga ggcggcgtcg gggtcggcgt cggcgtcgtg cccgccgaga
      721 tgaaggcgtt gtagaggagg cggttcggcg agccggagcc cgggctcacg accttgccgg
      781 tcgtcgcgtt cgtgttgagg gccgactcga ccgcgggcgg cgtcgtcgcg ccgttcgcct
      841 ggaggtactg ggcggcggca ccggcgacgt gcggcgaggc catcgacgtg cccgagatcg
      901 agttcgtcga ggtgtcgttc gtgagccacg tcgagacgac ggacacgccc ggggcgaaga
      961 tgtccacgca ggtgccgtag ttcgcgaacg aggccttgcg gtcctgcgag tccgtcgccg
     1021 agacgacgat cgcctcggtc acgcgcgacg gcgagtagtt gcaggcgttg tcggcgacgc
     1081 cgagcggccc gccgttgccg gccgcgagcg cgtagacggt cccgtcggcg atcgagttcc
     1141 ggaccgccgc gtcgatcgtc gagctcgtgc cgatctgggt cccgaggctc atgttcgcga
     1201 cgtctttctc accggcggtg tgctgcgccg tcacccagtc gacgccggcg atgatgtccg
     1261 agccgacacc gtcgccgttg cagtcgagca cccggaccgc gacgagcgac gcgctcttgg
     1321 cgacgccgta cgtcgtgcca ccgacggtcc cggcgacgtg cgtgccgtgg ccgtcgcagt
     1381 cgctcgcgtc ggggtcaccg tcgacgaagt cgtagccgtg cgacgcgcgg cccccgaagt
     1441 cggagtgggt gatccgcacg ccgctgtcga tcacgtaagc ggtcgtgccg gagccggtcg
     1501 cgttgtagac gtaggagttg ctcagcggaa ggttccgctg gtcgatgcgg tcgaggccgt
     1561 agggcgggtt gctctgggtc gcgtaggcac gcacgaccgg gtcctcctcg acgagcttca
     1621 cccgcgggtc cgcgctgatc gccagcgcgc cggcgcggtc gatctcgatc gagaagccct
     1681 tgatcgcgtg gttgtagacc cagccgagct caccgtcgtg gatccgtgcg agagaggtcg
     1741 cgacgtcggg gaccgacggc accgaggact cgagcatcac gatgtagctg ccggggaccg
     1801 ccttgcctgc ggggaggatc cgcccccgct tcgccgccgc tcccgccgat gccggaagca
     1861 aagcgaccaa tagggctgcg gccagggcta tcgtcacgga cacgcgcgcg ctcgcgttgg
     1921 acctcatgta gtcccctctc gtcggctggt ttgactgagg tttcgtcgcc agtcgaggac
     1981 ttcccttctg gaaaacggac aaatcgggtc cttttcgcgg gacagcttgt ccaacgaaag
     2041 gattagcaag tcaatcgatc gggtgattgc attaggggca atagctgaac gagcgcggtc
     2101 atactcggcg gcagcagagc tccgatcgac gagaccagac aaggagcgcg atgggcgaga
     2161 tccaggaggt gaagctcccc ggggtcggcg tccgctacga gttcgagacc gccgaaggca
     2221 acaggatcgg cgtggtctcg caccgcagcg gcctgcgcga ggtctacggg gcgaagcgcg
     2281 aggacccgga cgagttcaag cgcttgctcg gcctgtcgcc cgacgacgcg cggacgctcg
     2341 cagagctcct cggtgccacg cgcgtcgcgg agcagctggc ggagctccag caacggatcg
     2401 agggcctcgc gatcgactgg ctgcccgtgc gcgaggactc gacgtacgcg ggccggccca
     2461 tcggcgacac gaagatgcgc acgcgcaccg gcgtgtcggt cgtcgccatc gtccgcggcg
     2521 accaagcggt ccccgctccc ggccccgaag tgcacgtcga gagcggcgac tacctcgtcg
     2581 tcgtcggcac cgcgcggggt atcgaggagg ccgtcgctct gctccgcgcc ggctgagggg
     2641 agtggagcac ccggagcttc tagtcgagct gggggccgtg ctcctcggcc tcgccgtgct
     2701 ctcgcgcctc gcggtgcgca cggggatgcc gacgatcccg ctctacctgg ccgcggggct
     2761 cgcgttcggc gagggcggga tcgtcccgat cgtcaccgcg gacgagttcg tccaggtggg
     2821 ggccgagctc ggcttgatcc tcctgctgtt catgctcggg ctcgagtaca cggcgacaga
     2881 gctcctgacg acgatccgga gccaggcgcg cgtcggcgta ctcgacttcg tcctgaactt
     2941 cccgccgggg ttcttcgcgg cgctgatcct cgggtggggg ccgacgctcg gcgtcgtgct
     3001 cggaggcgtc acctacgtct cgtcgtccgg catcgtcgcg aagatcctct ccgaccaggg
     3061 atggaccggc aaccgcgaga cgcccgtggt cctgtcgacg ctcgtgatcg aggacctggt
     3121 gatggcgctc taccttcccg tcgccggcgc gctgctcgcc gggtccggag ggatcgcgtc
     3181 gttgctcccc gccttcggcg cgatagccgt ggtcgcggtc gtgctggcgg cggcgacgcg
     3241 cgtcgaggtc gggatcagcc gggtcctgtt cagccgctcg gacgaagcgc tgctgctgac
     3301 gatcctcggg atcacgatcc tcgtcgccgg gggggccgag ctcttcggcg tctcggcggc
     3361 ggtcgccgcg ctcctggtcg ggatcgtgct ttccggcccg gccgccgagg ccgcgcacgg
     3421 gttgctcacg ccgatgcgcg atctcttctc ggccctgttc ttcgcgttcg tcgggctcgc
     3481 cgtcgacccg gcgacgatcc cgccggtcct gctcccggcg gccgcgctcg cggtggtcac
     3541 gttcgcgacg aaggccttga ccggctggat gggggccgcc cgtgcgggtg tcggcgtaag
     3601 gggcgcgacg cgtgcgggct tgttgctcgc ggcgcgcggc gagttctcga tcgcgatcgc
     3661 gggcttcgca accgctgccg gggtcgccgg cgacttcgag gcgctcgcag tcgcatacgt
     3721 gttcctgctc gcgatcgccg gccccgttct cgtgaggttc gccgacgcga tcagcgctcc
     3781 gctcgtccgg ccctgacgat tgcatagctt tcccggtgtc gctcgaaagc ctcgagctgt
     3841 tgacttgtgt cgttccagga cggacgatgg gttcgaaggc cgagccgtgc gcccgacgac
     3901 cacacatctc tttcgagggg gcgcgcgtga gcgtgaagcc aactttctgc tcgacctgca
     3961 acaggtccgt ctacgtcggt cataccgact cgctcagctg cccggtgtgc tcgacgccgt
     4021 tgctcgagac ggtaggggtc gaagaggacg cccggtgagc ccgacggtgc tcgtcgtcga
     4081 cgacgacgag gaggtccggg acgcgctgag gctgctgttc gagctcgacg ggttctcggt
     4141 cgtgggggag gccgcgcacg gcgtcgacgg cgtcgcgctc gcacaccgcc accagccgga
     4201 cttcgtgatc ctcgactcgg tgatgccgta cctcggcggc gcggggacgg cgtcgatcct
     4261 gagggccata gcgccgtcgt cgaagatcgt cgcgttctcc gcggccctcg acagccgccc
     4321 ggcctgggcc gacgcgttcc tcaacaagga ccgcgtcggt gagatcgccc cgttcgtcac
     4381 cgccatgatc ccggcgcgac cccgaaggga gccggcgggt acgttggtct gatgcagcag
     4441 gtgaggccgg gcaccgtccc ggtcgtcctc tacgacgcgg actgtggctt ctgcacctgg
     4501 gtcgtcgatc ggatcgccgc ccgggtcgcc cccggggccc tcgagatcgt gccgctgcag
     4561 tcgacccgcg ccgacgacct gctcgggggc cgcgtcgacg aggccgccaa gtgggagtcg
     4621 tggcacctcg tcgaggccga cggatcgctg cactcgggcg gtgaggccgt cccgctggtc
     4681 ctccgtcacg tgaggggcgg ccgcgcgatg ggccgggtcg ccgcacggtt cccgcgcgca
     4741 accgccggcg cgtaccgcct cgtggcccgc aaccgcgacc ggctgggccg ggtcgtgcgg
     4801 gccgaccgct gccgggtccc gggcgccgat tagtttcgcc ggccgctccg gtcttagcct
     4861 tcgacggagc ccccccgagc cgaggagacc ctcatgcaga agatcgtccc gcacctgtgg
     4921 ttcgacacgg aggccgagga cgcggcgaag ttctacacgt cgctgttccc gaactcgagc
     4981 atcaccgacg tcagccacta cggcgaagcg gggccgcgcc cggcggggac cgtgctaacg
     5041 gtctcgttcg agctcgacgg ccaggagttc accgccctca acggcgggcc cgagttcaag
     5101 ttcaacgagg cggtctcgtt cctcgtgaac tgcgagacgc aggaagaggt cgacgacctc
     5161 tgggacagga tcctcgagtc gggcggcgag gagagccagt gcgggtggat caaggacggc
     5221 ttcgggctgt ggtggcagat cgtcccgacg gcgctcccgc ggctcctcca gggcgaggat
     5281 tccgaccgcg ccaaccgcgt gatgcaggcg atgttccaga tgagaaagct cgacatagcg
     5341 acgctcgaga gcgcctgatc ggtccttgca tcgcggggtc ccggccccca ggctgtaggc
     5401 ccctacagat aggagtcccg ccatgcgctt cgtcgcagcc ctgatcgcac tgtcgtcctt
     5461 cgtcgcctgc ggcggcgacg ccgcgggcag cgaggcgttc tgcgacgcga cccgcaagat
     5521 catcgagctc ggcaacgtcg aggaggtccc gccggaggtc gacacgatgg tcgaggaggc
     5581 ccccgacgag atcaaggacg cgaccgagac cgtgcgggac gtcttcgtcc aggccttcga
     5641 ggaccgggac ctctctcagc tccagtccca ggagttccag gacgcggcca cggagctgcg
     5701 cgagtacgca gtcgagaact gcgacaacgt gagggacatc acgaacgagt agccggaacc
     5761 tcttcggggg ccgggtcgta taggggcgga agagatcgct cccgtggagg gccggccact
     5821 ggaggaccga gagctagtcg aacgggtgaa gcgcggcgac gtgatcgcgt acgagacgtt
     5881 ggtgcagaga catcagtccg ccgccttccg gaccgcgtac ctcgtcgccg ggtcggcgaa
     5941 cgaggccgaa gacgctgccc aggaaggatt cctcaaggcg ttctacgcga tcgaccgctt
     6001 ccgcagcgac gcgccgttcc ggccgtggct cctcgccatc gtcgccaacg aggcgaagac
     6061 acggctgcgg tccgcctcta ggcggcagac gctcgccctg aggctgaccg agggtcgtct
     6121 cgaaggaggt gcggtcccat ctccggagga ggcggccctc gcgcgctccg agaaggagcg
     6181 cgtgctcgag gcgctggggc acctcggaga ggacgaccgt ctggtgatcg gctaccgcta
     6241 cttcctcgag ctctctgaga aggagaccgc ggccgcgctc ggggtgcgcc gcgggacggt
     6301 gaagtcgcgc ctgtcgcgcg cgctggtccg gctgcgctcg gtcatggagg agggatcgtg
     6361 acgcgacgcc gcgaccccgc cctcgccgcg ctgctgcagg aggccgcgcg ggggaccgcc
     6421 ttccccgaga cgcccgcgct cgccgaaagc gtgcgcgaga ggatcgagcg ggggccgatg
     6481 ccggtcgcag aggtccgtct gccgcgcacc cggcccccct tgctccgccc cgttctcgtc
     6541 acgttgctgg cgatcgcggt cgccctcggc gtcacgctgt cgatctcggt cacggctcgg
     6601 cgtgccgtag cggacctcct cggcgtcgtc gggatccgca tcacgttcgg cgacgaccct
     6661 cccggctcgc cgaccccggc gcgccggatc cggctgggcg acgcggtccc gaggaagacc
     6721 gcgagcgagc tcgccggatt cagggtgctc gtaccgggcg ccgcggccgg gcggcccgcg
     6781 atctacttcg accgccgcgt cggcgaccgc gggatggtca cggtcgtcta cccacgcgct
     6841 gcggagacgc tcgcccacgt cgacctgctc gtctccgagt tcgtcgcgtc gcttcccgct
     6901 gaatacgtga agaagctcgg cacgctcggg tcgcggatca cgtacaccga cgtcaggggg
     6961 gttcccgctt actgggtctc gggcgatccc cactacttct tcttcaccga gcgcaacggt
     7021 gcggtccgtc aggagaccat ccggctcgcc ggcaacgtcc tgctgtggga ggaggacggc
     7081 gtcaccttcc gcgtcgaagg cgccggctct ctgcgagagg cccgacggct cgccgagagc
     7141 ctgcgctgag ggaaccaacc cggctcgggc gtcgtaatca cgtcgacgtc cacaccggcg
     7201 gaaggagacc tcatgactcg ccgtgtcccg ttgctgctcg ccgtcctgct aggcggggcg
     7261 gttgcgtttg cgcccggagt cgccgcgggc gggttctgcc acgccgaccc ggcggcccgg
     7321 atgaagacgt cgtcggcccc gagggtcacg atgatcgact gcgcgttcga gaccacggtc
     7381 acgtacgtcg accccggtga gaaagtccgc tgggtcaaca aggacaggtc cccgcacacg
     7441 gtcaccggcg cggccctctc gtggggcacc gaggagatgc tcgacatggg cgacgccgtg
     7501 acctactcgt tcgaagagga aggcgtgttc ccgtactact gcgagctgca ccccgcgatg
     7561 gtcggtgcgg tggtcgtcgg tgacggcggg gggcctgcgg gggccgctgg gaccggcgtg
     7621 aaaccggtcg acgacgccgc ttcggtcgcg gcgaccgaga ccgcccctgc cggggccagc
     7681 gacgggctgt cgccggccgt cctcgcgctg gcgctggcag cggccgccgg cgggctggtc
     7741 gtggtgacgc gcgccgtgtg ggcccggagg gcgggcgctg cgtcggcttc ctgagcgcgc
     7801 cgattagttt cgcctctccg cgtggtcaac acccactgac ggaacacccg cggaggaagg
     7861 agagaccatg gcgaacccgg tcgtgcactt cgaggccatc ggccccgacg ggcctgcgct
     7921 gatcggcttc tacggcaagg ccttcggctg gagcatcaac gccgacaacc cgatgggcta
     7981 cgggatggtg gacaacggcg gcgaaggcat caacggcggg ataggccagg ccccgccgga
     8041 agggaacggt caccacctca cctggtacgt gtgggtcgag gacatcgacg cggcgctcga
     8101 gacgatcgag aacctcggcg gcaagacgat caacccgaag atgacggtcc cgggcggtcc
     8161 gacggtcgcg cacttcgagg atccggcggg gaacttcgtc gggctcgtga cggggggccc
     8221 ggaggagatg ggcaccgccg ccgggtcgta gcgccgggcg tactctgggc ggcgtgaccg
     8281 cagcggagtg cacccatctc gaccggatac gcgacgt
//
LOCUS       DASQDO010000530         6555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_116806, whole genome shotgun sequence.
ACCESSION   DASQDO010000530 DASQDO010000000
VERSION     DASQDO010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116806"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VEV43_14560"
     CDS             <1..532
                     /locus_tag="VEV43_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24787.1"
                     /translation="RTAVDLAMRRARPGAAARVHDFEGFYTSGFPAVYRATLAFSGSS
                     DVAAEATPEAFVRAVARGRRLSRAPWALGWVMTTSLNVARRLARQHPQERGPESDAAH
                     ESDARWVERLDLVERLALLPPRQRQAVVLHYLGGFELAEVAQLMNLSIGGVKSNLFKG
                     REALRRTQEARDHADD"
     gene            519..1769
                     /locus_tag="VEV43_14565"
     CDS             519..1769
                     /locus_tag="VEV43_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HYP24788.1"
                     /translation="MPTIDRLADELQAAADVRVAESPPAPNVVDAVRRGTRRRLRNRV
                     AVATGVVAFAAAGLAAARVVAPSTVREPRRVEVASGVRQIGRMTVPRAAHAATLLDDG
                     TVLLSGGFSGDTEIYYDSAEVFDPATRTFLPAAPMATARTGHAGVLLDDGTVLVAGGW
                     GGDFLSSAELYDPATRTFAATGSMAVARDGFTATKLRDGRVLVVGGFRESYETFNRSA
                     EIYDPETGEFTSTGSLARARASHTATLLRDGRVAVAGGLGPGGVLRSIEVYDPATGTF
                     TAGGRLTVARQKHGAVLLDRGRVLIVGGADIDDAFGRRRSAEVYDQATGVARKAGGMR
                     FARFKLPAPVNLPARGEVLVAGGADVPEVYEERSRSFVRVEGEHPGAHSFATATLLDD
                     GSVLIAGGYDDEVTATDGAWLFTP"
     gene            complement(1823..2014)
                     /locus_tag="VEV43_14570"
     CDS             complement(1823..2014)
                     /locus_tag="VEV43_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24789.1"
                     /translation="MKKRIAGLALAAALVALPVAPAQAAPAQGSDCPACLVGCLVNWT
                     FDYVTWGLQHPGYTCADVG"
     gene            complement(2089..3426)
                     /locus_tag="VEV43_14575"
     CDS             complement(2089..3426)
                     /locus_tag="VEV43_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24790.1"
                     /translation="MADPKIEKRPDVGVVFDVQRFERPGGDGVASGFQPTGPWDFPDG
                     GYDHFVEHGVQKVRIRPAAPTNGDVGIERHFEVSPGDVYRLTARVRVARKDGAFKGRV
                     NLSAREENGAQVEEFNGAQEEQTDTPMERTALAVVPKEARVLTARVKFHSSAPGDAGE
                     GEIHSVRLERLRDVPKEPEGERLSLYDFNVHKMEDEWRGWIRFIKDQRLAPPDIVLLQ
                     DIADDAGRMRFEDAMGEAFGGHWAGRGSDPAWQVAVVWRSGRFSQAQSRVWRGFGGEG
                     CVDGSQDAPALQLKLRDDAARKWISVVSLKTPPGVADECVWKNMQKVDGNFDGAWRGD
                     LCVIGTDANSPDRDDRDDWARWYRRTIRSEAAHLAAEDSLGFCDPVADECGRDRARME
                     DHLTLGKMRIDFLMLRTGGRKAPRVVRQMTLPRGNVDGVKWSDHRSVHAEVVY"
     gene            complement(3436..3876)
                     /locus_tag="VEV43_14580"
     CDS             complement(3436..3876)
                     /locus_tag="VEV43_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYP24791.1"
                     /translation="MSETEPMDVPKVLELLSKAIPLQMRSTLQLAWVAGSAIGVEFQS
                     IAVKFEEFSQAELHDTRRLMEKMMALGGTPPTEVAPLEAVPSSTDGVKRLIELEQESL
                     EALHAIIPATGQEARSEALEHLLEHVIMRKQHQIDFLLRAAREA"
     gene            3907..5745
                     /gene="typA"
                     /locus_tag="VEV43_14585"
     CDS             3907..5745
                     /gene="typA"
                     /locus_tag="VEV43_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HYP24792.1"
                     /translation="MLTRDDLRNVAIVAHVDHGKTTLVDAMLWQSGAFSSHQDVADRV
                     MDSMDLEREKGITILAKNTAVRYGDVKINIVDTPGHADFGGEVERGLTMVDGVLLLVD
                     ASEGPLPQTRFVLRKALEGRLPVILVVNKVDRPDARIADVVNEVYELFLDLDADEGQI
                     DFPIVYCNAKAGWASMDPEQEGSDLKPLFELLLDHIPAPAYEEDHPLQALVTNLDASP
                     YVGRLALCRVFHGSIRRGEQVAWCRADDTIERVKITELYVTEALDRVDAAEAGPGEII
                     AIAGLAEVTIGETLSDPEDPRPLPVSHFDDPSLSMTIGINTSPLSGQEGEKLTARLVK
                     NRLDAELVGNVSIRVLETERPDAWEVQGRGELQLAVLVEMMRREGFELTVGKPQVVTR
                     VVDGKVHEPVERLTIDVPEDFMGVVTQMLALRKGRLENMVNHGTGWVRMEYLVPARGL
                     IGFRTEFMTETRGTGLLHHVFDRYEPWHGELRTRPTGSLVADRRGPTTSYALLNLQDR
                     GTMFVPPGVEVYEGMIVGENTRGEDMDVNPTKEKKLTNMRSSTSDELVRLIPHKEMSL
                     EQALEFIREDECAEVTPRTVRLRKVILDAAGRARAGKRGKGTPRAT"
     gene            5792..>6555
                     /locus_tag="VEV43_14590"
     CDS             5792..>6555
                     /locus_tag="VEV43_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYP24793.1"
                     /translation="MRRTCASIVAFAAMALPAPALGAPARADAVVAVIDTGINPYHDT
                     FADESPRGHRHPSTYIDGFPKDAQALRLTLDEPDFEKAFLADCKRVWAKVEPGRLYWF
                     PGTKIVGAITFGPQSPAGCTSWRTTSGRILDASGHGTMTASRAASNEYGACRSCRIVA
                     IQTGYDYVSGGFAGAVGFAGDNAGWIDAQSNSWGPVAPVWDPTNATVIWAATTELIEA
                     VERSARAHLSFWASGNGAATRGGAVGHPAVLQPPMTP"
BASE COUNT          988 a         2295 c         2306 g          966 t
ORIGIN      
        1 acgcactgcg gtggatctag ccatgaggcg ggcccggccg ggggccgcgg cccgcgtcca
       61 cgatttcgag ggcttctata cgtccgggtt cccggccgtc taccgggcaa cgctcgcgtt
      121 ctcgggctcg tccgacgtcg cggcggaggc cacgccggag gccttcgtcc gggcggtcgc
      181 acgggggcgg cggctgagcc gagcgccctg ggcgctcggg tgggtcatga cgacgtcgct
      241 caacgtcgcg cgccggctcg cgcgacagca cccgcaggag cgtggcccgg agagcgacgc
      301 cgcgcacgag tccgacgcgc gctgggtgga gcgcctcgat ctcgtcgagc gtctcgcgct
      361 gctcccgccg cgtcagagac aagccgtcgt cctgcattac ctgggcgggt tcgagctcgc
      421 cgaggtcgcg cagctgatga acctctcgat cggaggcgtg aagagcaacc tcttcaaagg
      481 ccgggaggcg cttcgccgca ctcaggaagc gagggaccat gccgacgatt gatcgcctgg
      541 ccgacgagct ccaggccgcc gccgacgtcc gggtagccga gagcccgccc gcacccaacg
      601 tggtcgacgc cgtgcgtcga ggcacgagaa ggcggctgcg caaccgggtc gcggtcgcca
      661 cgggcgtcgt cgcgttcgcg gcggcggggc tggcggccgc acgcgtggtc gcgccgtcga
      721 cggtccgaga gccgcgccgt gtcgaggtcg catccggcgt ccggcagatc ggaaggatga
      781 ccgtgcctcg agccgcgcat gcggcgacgc tcctcgacga cgggaccgtg ctgctgtcag
      841 gcgggttcag cggcgacacc gagatctact acgactccgc ggaggtcttc gatcccgcga
      901 ctcgcacgtt cctgccggcc gcgccgatgg cgactgcgcg caccgggcat gcgggcgtgc
      961 tgctcgacga cgggacggtg ctggtcgccg gaggatgggg cggggacttc ctgtcgagcg
     1021 cggagctcta cgacccggcg acgaggacgt tcgccgcgac cggttcgatg gcggtcgcgc
     1081 gcgacggctt caccgctacc aagctgcgcg acggccgcgt gctggtcgtg ggcgggttcc
     1141 gcgagagcta cgagacgttc aaccgcagcg ccgagatcta cgaccccgag accggggagt
     1201 tcacgtcgac cgggagcctg gcgcgcgccc gcgcttcaca caccgcgacg ttgctgcgag
     1261 acggcagggt cgcggtcgcc ggtgggctgg gacccggcgg tgtgctgcgg tcgatcgagg
     1321 tctacgaccc ggccaccggg acgttcacgg ccgggggccg gctgacggtc gcgcggcaga
     1381 agcacggcgc cgtcctgctc gatcgcggcc gggtcttgat cgtcggtggt gcagacatcg
     1441 acgacgcctt cggccgccgt cgtagcgcgg aggtctacga ccaggccacg ggggtcgctc
     1501 ggaaggccgg gggcatgcgc ttcgcgcggt tcaagctccc cgcaccggtg aaccttcccg
     1561 cgcgcgggga ggtcctggtg gccgggggag ccgacgtgcc cgaggtctac gaggagcgct
     1621 cacggtcgtt cgtccgggtc gagggcgagc accccggcgc tcattcgttc gcgaccgcga
     1681 cgttgctgga cgacggctcg gtgctgatcg cgggtggcta cgacgacgag gtcacggcca
     1741 ccgacggggc ctggttgttc accccctgaa cgcaagagat gcccccgtcc ccggaggcat
     1801 ctcccgttcc gagcgaaatt acctagccga cgtccgcaca ggtgtagccg gggtgctgca
     1861 gtccccacgt cacgtagtcg aaggtccaat tcacgaggca ccccaccaga caagccgggc
     1921 agtccgaccc ctgcgccggt gccgcctgag ccggtgccac ggggagcgcc accagcgccg
     1981 ccgcgagggc caggcccgca atcctcttct tcatggttcc ctcctctcgt ccccatcgga
     2041 aaagggtcaa agcgcacatt tcggcacgcg ggggcccggg acctcccgct agtacacgac
     2101 ctccgcgtgg accgaccggt ggtccgacca cttcacgccg tcgacgttgc cgcggggaag
     2161 cgtcatctgc cggacgacgc gcggggcctt gcggccgccc gtcctcagca tgaggaagtc
     2221 gatccgcatc ttccccagcg tgagatggtc ctccatccgc gcgcggtcgc gcccgcactc
     2281 gtcggcgacc gggtcgcaga acccgagcga gtcctccgcc gcgagatgcg cggcctcgga
     2341 cctgatcgtc cttcggtacc aacgggccca gtcgtcgcgg tcgtcgcgat ccggcgagtt
     2401 cgcgtcggtc ccgatcacgc agaggtcgcc gcgccaggcg ccgtcgaagt ttccgtcgac
     2461 cttctgcatg ttcttccaca cgcactcgtc cgcgacgccc ggcggagtct tcaggctgac
     2521 gaccgagatc cacttgcgcg cggcgtcgtc gcggagcttc agctgaaggg caggcgcgtc
     2581 ctggctgccg tcgacgcagc cctcgccgcc gaacccgcgc cacacgcggc tctgggcttg
     2641 cgagaaacgc ccgctgcgcc acacgaccgc gacctgccac gccgggtcgc tcccgcggcc
     2701 cgcccaatgt ccgccgaacg cctcgcccat cgcgtcctcg aagcgcatcc ttccggcgtc
     2761 gtcggcgatg tcctggagga gcactatgtc gggaggcgcg agccgctggt cctttatgaa
     2821 ccggatccag ccgcgccact cgtcttccat cttgtggacg ttgaagtcgt agagagagag
     2881 cctctcgccc tccggctcct tcgggacgtc ccgcaggcgt tcgagacgca ccgagtggat
     2941 ctcgccttcg ccggcgtcgc cgggagcgga agagtggaac ttgacgcgtg ccgtgaggac
     3001 gcgcgcttcc ttggggacga ccgcgagcgc ggtccgttcc atcggggtgt cggtctgctc
     3061 ctcctgcgcg ccgttgaact cctcgacctg cgcgccgttc tcttccctcg ccgagaggtt
     3121 gacccggccc ttgaacgccc cgtccttgcg ggccacgcgc acgcgcgcgg tgaggcgata
     3181 gacgtcgccc ggggagacct cgaagtgccg ttcgatgccg acgtcgccgt tcgtgggggc
     3241 cgcggggcgg atgcgcacct tctgcacgcc gtgctcgacg aagtggtcgt aaccgccgtc
     3301 ggggaagtcc cagggcccgg tcggctggaa tccggacgcg acgccgtcgc cgcccggccg
     3361 ctcgaaccgc tgcacgtcga acacgacgcc gacgtccggg cgcttctcga tcttggggtc
     3421 cgccacccct agaagttacg cctccctagc cgcccgcaac aagaagtcga tctggtgctg
     3481 cttgcgcatg atcacgtgct ccagcaggtg ctccagggcc tccgagcgcg cctcctggcc
     3541 cgtcgccggg atgatcgcgt gcagcgcttc gagcgactcc tgctccagct cgatcagccg
     3601 cttcacgccg tcggtcgacg acgggacggc ctccagcggc gcgacctcgg tcgggggcgt
     3661 cccgccgagc gccatcatct tctccatcag gcggcgcgtg tcgtggagct cggcctgcga
     3721 gaactcctcg aacttcaccg cgatcgactg gaactcgacg ccgatcgcgc tgccggcgac
     3781 ccacgcgagc tgcagcgtcg aacgcatctg cagggggatc gccttgctca gcagctccaa
     3841 taccttcggt acgtccatcg gttcggtttc gctcatgcgg cgagtgtacg gacctacact
     3901 ggctacatgc tcacccgcga cgacctgcgc aacgtcgcga tcgtcgccca tgtcgaccac
     3961 ggcaagacga cgctcgtcga cgcgatgctc tggcagtccg gcgcgttcag cagccaccag
     4021 gacgtcgccg accgcgtgat ggactcgatg gacctcgagc gcgagaaggg catcacgatc
     4081 ctcgccaaga acaccgcggt ccgctacgga gacgtgaaga tcaacatcgt cgacacgccg
     4141 ggccacgcgg acttcggcgg cgaggtcgag cggggcctga cgatggtcga cggcgtgctg
     4201 ctgctcgtcg acgcgagcga gggccccctg ccccagaccc gcttcgtgct gcgcaaggca
     4261 ctcgaggggc ggctgccggt gatcctcgtc gtcaacaagg tcgaccgtcc cgacgcgcgc
     4321 atcgccgacg tcgtgaacga ggtctacgag ctgttcctgg acctcgacgc agacgaaggc
     4381 cagatcgact tcccgatcgt gtactgcaac gcaaaggccg gctgggcctc gatggacccg
     4441 gagcaggagg ggagcgacct caagccgctg ttcgagctcc tgctggacca catcccggcc
     4501 cccgcgtacg aagaggacca cccgctgcag gcgctggtca cgaacctcga cgcgtctccg
     4561 tacgtgggga ggctggcgct ctgccgggtg ttccacggat cgatccggcg cggcgagcag
     4621 gtcgcgtggt gccgtgccga cgacacgatc gagcgcgtga agatcaccga gctgtacgtg
     4681 accgaggccc tcgaccgcgt cgacgccgcc gaagccgggc cgggcgagat catcgcgatc
     4741 gccgggctcg ccgaggtcac gatcggcgag acgctgtccg accccgagga tccccggccg
     4801 ctgccggtca gccacttcga cgaccccagc ctgtcgatga cgatcggcat caacacctcg
     4861 ccgctgtcgg ggcaggaggg cgagaagctg accgcgcggc tcgtgaagaa ccgcctcgac
     4921 gcggagctcg tcggcaacgt ctcgatccgc gtgctcgaga ccgaacgtcc ggacgcgtgg
     4981 gaggtgcagg gcaggggaga gctgcagctc gccgtgctcg tcgagatgat gcggcgcgag
     5041 ggcttcgagc tcacggtcgg caagcctcag gtcgtcacgc gcgtcgtcga cgggaaggtg
     5101 cacgagcccg tcgagcggct gacgatcgac gtcccggagg acttcatggg cgtcgtcacg
     5161 cagatgctcg cgctgcgcaa ggggcgcctc gagaacatgg tcaatcacgg caccggctgg
     5221 gtgcgcatgg agtacctggt gcccgctcgg ggcctcatcg gcttccgcac cgagttcatg
     5281 acggagacac gcggcaccgg gctgctccat cacgtcttcg accgttacga gccgtggcac
     5341 ggcgagctga ggacgcggcc cacgggaagc ctcgtcgccg accggcgcgg gccgacgacg
     5401 tcgtacgcgc tgctgaacct gcaggaccgc ggcacgatgt tcgtccctcc cggcgtggag
     5461 gtctacgagg ggatgatcgt cggcgagaac acgcgcggcg aggacatgga cgtgaacccc
     5521 acgaaggaga agaagctcac gaacatgcgg tcgtcgacga gcgacgagct cgtgcggctc
     5581 atcccgcaca aggagatgtc gctcgagcag gcgctcgagt tcatccgcga ggacgagtgc
     5641 gccgaggtga cgccgcgcac ggtgaggctg cgcaaggtga tcctggacgc ggccggtcga
     5701 gcgagggccg ggaagagagg caaaggcacg ccccgggcca cttgaggccg ccggaggggg
     5761 accggacggc ccggcgccga agccttctca tatgcgtagg acctgcgctt cgatcgtcgc
     5821 gttcgcggcg atggccttgc ccgccccggc tctcggtgcg ccggcccggg cagacgccgt
     5881 cgtcgcggtg atcgacaccg gcatcaaccc gtaccacgac acgttcgcgg acgagtcgcc
     5941 gcgcggccac cggcatccct ccacctacat cgacggtttc ccgaaggacg cgcaggcgct
     6001 gaggctcacg ctcgacgagc ccgacttcga gaaggccttc ctcgcagact gcaagcgcgt
     6061 gtgggcgaag gtcgagcccg gccgcctcta ctggttcccc ggcacgaaga tcgtcggcgc
     6121 gatcactttc gggccgcagt cgccggcggg gtgcacgagc tggcggacca cctccggccg
     6181 gatcctcgac gcgtcgggtc acggaacgat gacggcgtcg cgggcggcct cgaacgagta
     6241 cggcgcgtgc aggtcgtgcc ggatcgtcgc gatccagacg ggatacgact acgtctcggg
     6301 agggttcgcc ggtgcagtcg ggttcgcggg agacaacgcg ggatggatcg acgcgcagtc
     6361 gaactcgtgg gggccggtcg cgccggtctg ggacccgacg aacgcgacgg tgatctgggc
     6421 ggctacgacc gagctgatcg aggcggtgga acgcagcgcc cgcgcacacc tctcgttctg
     6481 ggcttcgggc aacggggccg ccacgagggg cggcgccgtg gggcacccgg cggtcctgca
     6541 gccgcccatg acgcc
//
LOCUS       DASQDO010000531        10657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1217550, whole genome shotgun sequence.
ACCESSION   DASQDO010000531 DASQDO010000000
VERSION     DASQDO010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217550"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..469
                     /locus_tag="VEV43_14595"
     CDS             233..469
                     /locus_tag="VEV43_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP24794.1"
                     /translation="MLQLSGRPVTGARGWDALTAAELRVIALVTEGLTNPQIAARLFV
                     SAQTVKTHMKNVFRKLGVSSRAELAALATRKGIG"
     gene            509..2158
                     /locus_tag="VEV43_14600"
     CDS             509..2158
                     /locus_tag="VEV43_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HYP24795.1"
                     /translation="MKKLIVLALASLLVSALAPIGRTDASVLSIARVRVDSPEQAGYL
                     MSHFDETHNHGRNEIELLLWPGDLAELDSLGLDYEVVVEDLVAHERALEAAAPDVVSV
                     QPGPDRTDYRRLADYNAEMQELADKNKGLVELFEMERPSLEGRTIYGLEIAANVKDKL
                     DGRPVFYLDAVHHAREWPASEFTMIFAHHLVESYGKDPKITGLLKRARVVVVPVVNVD
                     GFDYSRESVTSAHPTLASNTDVLSGPQGFEGYWRKNRRSLTGVTVPAAQRNPDAYGVD
                     PNRNYSYLWGDNQGGSSGNQLDATYRGAAPFSEPEVANVRDIVLSRNVTGVITNHTYQ
                     SSVLRAGGGKSPEDKILFSLGQKMADVLGYQNNGTVGYPTTGTTDDWAYAVTGPLGFT
                     MEHGTQGFHPPYGDFVAQHHEKASKAFTIMLEAVANPRYHSVLTGRVDGGPAKLTLTK
                     TVRVPLSEGNPIGEEFVVEKIKYTMAVGKDGRFEWHVGPSTSPWNTKTNEAYTLTVTA
                     PSGGSMKMPLTIDRGQKRDLGTINLSRPAQAVKSDGHAHTH"
     gene            complement(2292..3512)
                     /locus_tag="VEV43_14605"
     CDS             complement(2292..3512)
                     /locus_tag="VEV43_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24796.1"
                     /translation="MQTGRPLRFLFLAALLVAGLPAGPARAQLDCHDREVTIEGTDGD
                     DELTGTAGSDVILGLEGNDVIRGLGGDDFLCGGEGIDRIFGGGGEDSLEGGAGDDADS
                     AIVSLNGQDIGLELDGGLFGERGNDVLSGDAGTDTVLGGYGNDVVDGGAEDDVGIGGP
                     GNDGVFGAGGSDFLRGDKGDDRVDAGTTDGLIGASDEDLHGGPGNDVLQVRGGDGNAF
                     GGPGNDRIDGGEGALNASGGKGNDVVRSGPADDILYGTAGRDRLVSGGDEDDVFGGSG
                     RDVLIVGGAADSAYGEGGADRLVGGEGEDNLNGLDGNDVLRAGPGPDNAFGGGGNDKL
                     FTAAGEDAAHGGAGNDVLRTGAQGDDAFGDIGRDRIFTGAGEDSIDGGDGRDRANGGP
                     GSDECLSVERARSC"
     gene            3695..4570
                     /locus_tag="VEV43_14610"
     CDS             3695..4570
                     /locus_tag="VEV43_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYP24797.1"
                     /translation="MARADELKDQSLRLGDVADVIGVPGTTIRAWETRYGWPNPARTS
                     GRHRRYSAEDLRRLVALRNEIARGRNAGEAVEMLRAIDARRAHEYVDPIVAGAISLDP
                     GRVRSTLLDAERELGVVRTVENVAVVALREIGDHWAVGTCDVAGEHLGTGEIRRWFGE
                     AAARSFDDTSGRPVVLACGPEELHTGGLEAFRVLLVERGVPALLLGAQTPPDSFALTV
                     RDSGAAAAVMLCHRAQARRGSLVTLRLLSSIPKTKVFYAGNGFAAARGRRSVPGTYLG
                     TDLSAAADLVAGAIA"
     gene            4623..5288
                     /locus_tag="VEV43_14615"
     CDS             4623..5288
                     /locus_tag="VEV43_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP24798.1"
                     /translation="MSDAVIEAQGIVRTYSSGAEAVHALRGVDLAVMPGEFLAITGAS
                     GSGKSTLLHVIGGLDRPDDGRVVLEGRDMSSLPDEELALVRRRHLGFVLQFFNLLPTL
                     TAVENVAFPLLLDGHPDALPRASESLGSVGLSERADHRPSQLSGGEQQRVALARALVT
                     KPAVVLADEPTGNLDSATGEGILGLLRATADRGQTIVMVTHDGRTAEYADRTVRLADG
                     RLT"
     gene            5285..7873
                     /locus_tag="VEV43_14620"
     CDS             5285..7873
                     /locus_tag="VEV43_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYP24799.1"
                     /translation="MKGLLAFLRRVNGRHLVEKRLRTALTIGGVAAGVALVTSITVIN
                     STLLSSVREGIRNLAGAAEIEVASADETGLPEDVVAEVAAVGGVEAAVPVVRTTAHLT
                     GPAGESDTMVLGVGPGFLRLFPTGLGDAGRVVVEGELGLRARGVVLAEQVAERLGAEI
                     GDVVRAETPSGSVALQVTAHASGPGLSLLNGGDVGTMSLEAAQEAFEKRGRVDSVYVV
                     VEEDVAAGRVESALDDVLGGAAIVGPPGERGEAFERTFESIAALTSLAGAVSLFVALF
                     VVYNTMSIALAERRREVSMAIALGAKRSQLFAAFLGEAGLLGTVASVAGVGAGIVLAR
                     VLVGRAVDQFAAVGVRATGPLAVEPWHVLLGLGAGIAVSLAGAFLPARRIMRVAPVEA
                     LRPEAGLEWARTRRGALTPARSVALAVGGVIVCLGGMALYIETGAYPVAVVSLAAGLG
                     GVTTILPVAVPLGVRVLRPVFTRLFGATGRLAADALAKNPGRTSVTVGSLVLTLGLVI
                     GVGGAIGSLDRQFRDFAQHWYSQPLYVQTNSYTGGVESDQPLSGKFVAALEEVEGVDN
                     VLPFRYANLTADGEQVIAYVHPTSGEARADAVEGLSVGDVDSEPLVDALERGEVVVAE
                     QMAQRRELEIGDTLSIAGPQGLKDFTVGGFFNDLASFDSMYMSADTYREHWNDDRADS
                     FGVDPEPGVDVAALERRLEETVEAEGMPAQVIRKEEQVENITATVDQIFSIAEGIQLA
                     AVLVAFLTIVNTMFTAVIERRWEFGLQQAIGMGRRQLGRTVVLEAAGIGVVGGVGAVV
                     LGSGLGVLMLVSMNFLYAFDIPYQPPWTLFAVAIAAGTLIAALSAVYPRRLATRLRIV
                     ECLRYE"
     gene            7855..9243
                     /locus_tag="VEV43_14625"
     CDS             7855..9243
                     /locus_tag="VEV43_14625"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYP24800.1"
                     /translation="MPPLRVGGSRPRRLLGVLLAGCFGAAASYAALGLLPAVRHEAGP
                     ATLDTTLAPGGGRMVLRIPPLGTVTAGTHTPPVALRMTLSEVDVPRLTERVGNAAARA
                     ELAAAVEEDLRALVPRLVWQTCLGAAVLGGIVAALLPRRRLSYVAAGAAGGAVAAAAL
                     LGLTAASFDVDAFEQPSFTGALVRAPVVIDALQSGEVTLGAIQSRYETAADRLSELLA
                     LVAEPGQDPAGDSVAILHVSDIHSNPLGVQLARRLAERFDVDAVLDTGDLTNFGVTLE
                     SRIGQLVERIDVPYYLVPGNHDSAANEAALDRVENLTILDGTVADVSGVSILGWGDPT
                     DTNWNNISPDEAAEIRVEEGEDAAAEVARVAPDVLAVHDSRMAEASYGAVPLVLSGHY
                     HRRIVEEEDGTTVLGVGSTGATGLKFFVEAARDYEAEILYFRDGVAVAVDYVRFSGLG
                     TEFEIERDALDE"
     gene            9288..9929
                     /locus_tag="VEV43_14630"
     CDS             9288..9929
                     /locus_tag="VEV43_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24801.1"
                     /translation="MEASLTVRSVRLVAGLWLFALGTVLTLRASLGVPPWDVLHDGIR
                     RNTPLSFGIAVIAIGLLLVLVSALFGIRPGPGTIANMLLVGTFVDLMLLSDAGTGLED
                     AHMAWRVAVAFAGVAVVGLGSALYIGANLGAGPRDSLMVLAATKLGVRVGVARAVIEG
                     TALLVGWLLGGAVGIGTLLFVVTIGPSVDVFFRLFRMEADGRRAGPVALDTSA"
     gene            9974..10282
                     /locus_tag="VEV43_14635"
     CDS             9974..10282
                     /locus_tag="VEV43_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24802.1"
                     /translation="MDEQGRVVELAIFKLKEGVGRDQLMETVDAVTEWAKRQPGFVSR
                     DLTYSAESDTWVDVVWWESIESAHAASEAAMTSESCAPMFGLIDLEGTQMIHGERVAA
                     "
     gene            10298..>10657
                     /locus_tag="VEV43_14640"
     CDS             10298..>10657
                     /locus_tag="VEV43_14640"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HYP24803.1"
                     /translation="MLQVTWEWLGMPPALDLANTVAITEGAEQDLVGSNEDFEEWARI
                     ESSFVPAPVGLLRLHRSELMEVRAAVRDLLAAVTAHEPPPRRAIETLNRLSRAAPEWT
                     ELDAASWRVVTRTGAGAA"
BASE COUNT         1458 a         3781 c         3861 g         1557 t
ORIGIN      
        1 ttcagcgtcg gagtcgcccg cttcgacccg gcgaacccgt cgacgatcga ggagctcatg
       61 cgcgcggcgg acgccgcgat gtaccgggac aaggccgcca agcactccgg ctcctagacg
      121 cccgggctgg cccatgtagg ccaagccttg cgcctcaact acagcagtag tagaaagagg
      181 ctcgaacagc ttccgaatct cctttccggg gagctcgagc cgtgggggaa ccatgctgca
      241 gctgtctgga cgtcccgtga cgggcgcacg aggatgggat gcgctcaccg ccgcggagct
      301 gagggtgatc gctctcgtga ccgaagggct gacgaaccct cagatcgcgg cccgcctgtt
      361 cgtgtccgcc cagaccgtca agacgcacat gaagaacgtc ttccgcaagc tgggggtgtc
      421 gagccgggcc gagctggccg cgctggcgac ccggaagggc atcggctagg cgggaggaac
      481 cgccggggag gcggcgaacc ccctccccat gaaaaagcta atcgttctcg cgctcgcctc
      541 cctgctcgtc tccgccctgg cccccatcgg ccggaccgac gcttccgtcc tctcgatcgc
      601 gcgcgtgcgc gtcgacagcc cggaacaggc cggctatctc atgtcgcact tcgacgagac
      661 ccacaaccac ggccggaacg agatcgagct gctgctctgg cccggcgacc tcgccgagct
      721 ggactcgctc ggcctcgact acgaggtcgt cgtcgaggac ctcgtcgctc acgagcgtgc
      781 gctcgaggcc gcggcacccg acgtcgtgtc ggttcagccc gggcccgacc gcaccgacta
      841 ccggcgcctc gcggactaca acgccgagat gcaggagctc gccgacaaga acaagggcct
      901 ggtcgagctg ttcgagatgg agcgtccctc gctcgagggc cggacgatct acggcctcga
      961 gatcgcggcg aacgtcaagg acaagctgga cggccgcccg gtcttctacc tcgacgcggt
     1021 gcaccacgcg cgggagtggc cggcgtcgga gttcacgatg atcttcgccc accacctcgt
     1081 cgagtcgtac gggaaggatc cgaagatcac ggggctgctg aagcgggccc gggtggtcgt
     1141 cgtgccggtc gtcaacgtcg acggcttcga ctactcgcgt gagtcggtca cgtctgctca
     1201 cccgacgctc gcgagcaaca ccgacgtgct gtccgggccg cagggcttcg agggttactg
     1261 gcgtaagaac cggcgctcgc tgaccggggt gaccgttccc gctgcgcaac gcaacccgga
     1321 cgcctacggc gtcgatccga accgcaacta ctcgtacctc tggggcgaca accagggtgg
     1381 gtcctcgggc aaccagctcg acgcgaccta tcggggcgcg gcgccgttct ccgagcccga
     1441 ggtcgcgaac gtgcgggaca tcgtgctcag ccgcaacgtg accggcgtca tcacgaacca
     1501 cacctatcag tcgagcgtgc ttcgggccgg cggcggcaag tcgcccgagg acaagatcct
     1561 gttctcgctc ggccagaaga tggccgacgt cctcggctac cagaacaacg gcaccgtcgg
     1621 gtacccgacg accgggacga ccgacgactg ggcttacgcg gtgacgggcc cgctcgggtt
     1681 cacgatggag cacggcaccc agggcttcca cccgccgtac ggtgacttcg tcgcccaaca
     1741 ccacgagaag gcgagcaagg cgttcacgat catgctcgag gccgtcgcga acccgaggta
     1801 ccactcggtg ctgaccggcc gcgtggacgg gggcccggcg aagctcacgc tgacgaagac
     1861 cgtgcgggtg ccgctgtcgg agggcaaccc gatcggcgag gagttcgtcg tcgagaagat
     1921 caagtacacg atggcggtgg gcaaggacgg ccgcttcgag tggcacgtcg gcccgagcac
     1981 gagcccgtgg aacacgaaga ccaacgaggc ctacacgctg acggtcacgg cgccgagcgg
     2041 cggctcgatg aagatgccgc tgacgatcga ccggggccag aagcgggacc tggggacgat
     2101 caacctgtcg aggccggccc aggccgtgaa gtcggatggc cacgcgcaca cccactagcc
     2161 gagatccgat gttcgcaaga gggggccccg aggggcccct tcttcgcgtc ggtcgccgcc
     2221 gccgcgaccg gacagcaggc gccgccggag ccgccgtcgc gcggcctcgg actagctggc
     2281 ggcgctctag ctcagcagga gcgcgctcgc tcgaccgata ggcactcgtc ggagcccggc
     2341 cccccgttcg cgcggtcccg gccgtcccct ccgtcgatgc tgtcctcgcc ggcgcccgtg
     2401 aagatgcggt cgcggccgat gtcgccgaac gcgtcgtcgc cctgggcccc cgtgcgcagc
     2461 acgtcgttgc cggcaccgcc gtgggcggcg tcctcaccgg cggccgtgaa cagcttgtcg
     2521 ttgccaccgc cgccgaacgc gttgtcgggg ccggggccgg cacgcaggac gtcgttgccg
     2581 tcgaggccgt tgaggttgtc ctctccctcg cctccgacga gccggtcggc gccgccctcg
     2641 ccgtaagccg agtcggcggc accgccgacg atcaggacgt cgcggccgct cccgccgaag
     2701 acgtcgtcct cgtcgccgcc ggacacgagc cggtcgcggc ccgcggtccc gtagaggatg
     2761 tcgtccgcgg ggccgctcct gacgacgtcg ttgcccttgc cgcccgacgc gttgagtgcg
     2821 ccttcgccac cgtcgatccg gtcgttgccg ggaccgccga aggcgttgcc gtcgcccccg
     2881 cggacctgga ggacgtcgtt gcccggcccc ccgtgcaggt cttcgtcgga cgcaccgatg
     2941 agcccgtcgg tcgtccccgc gtcgacgcgg tcgtcgccct tgtccccgcg caggaagtcg
     3001 ctgccgccgg cgccgaacac gccgtcgttg cccggcccgc cgatgccgac gtcgtcctcg
     3061 gctccgccgt cgacgacgtc gttgccgtat ccgccgagca cggtgtcggt ccccgcgtcg
     3121 ccggagagca cgtcgttgcc gcgctcgccg aagaggcccc cgtcgagctc gagcccgatg
     3181 tcctggccgt tgagcgagac gatcgcagag tcggcgtcgt cgccggcgcc accctcgagg
     3241 gagtcttcgc cgcctccgcc gaagatgcgg tcgatgccct cgccgccgca gaggaagtcg
     3301 tcgcccccga ggcccctgat cacgtcgttg ccctcgagcc ccaggatcac gtcgctgccg
     3361 gcggtgccgg tcagctcgtc gtcgccgtcc gtgccctcga tcgtgacctc gcggtcgtgg
     3421 cagtcgagct gcgcccgggc cggtccggcc ggcaggccgg ccaccagcag ggccgcgagg
     3481 aacaagaagc gcaacggtcg accggtttgc acgtgggtcc cctccttcgt acgacgccga
     3541 gcgcaagtta agcagcgagc gcgctccgcg tcagctactt gacccaaagc ggtctcgaac
     3601 gcgccacggc gggcgcggga aggggaccgg cgcattcggt tcccgaaggc tctcgtgggt
     3661 aagaggaaga aaaccatgca aagcgacgag tagcgtggct cgggcggacg agctcaagga
     3721 ccaatcactg cgactgggcg acgtcgcgga cgtcatcggc gtgccgggga cgacgatccg
     3781 cgcctgggag acgcgctacg gatggccgaa ccccgcacgc acgtccggcc ggcaccgccg
     3841 ctactccgca gaggacctgc gccgtctcgt cgcgctgcgc aacgagatcg cgcgcgggcg
     3901 caacgcgggc gaggccgtcg agatgctgcg ggccatcgac gcgcgccgcg cgcacgagta
     3961 cgtcgacccg atcgtcgcgg gtgcgatctc gctcgacccg ggccgcgtga ggagcacgct
     4021 cctcgacgca gagcgggagc tcggggtcgt gaggaccgtc gagaacgtcg ccgtcgtagc
     4081 gctccgcgag atcggtgacc actgggcggt cgggacgtgc gacgtggcgg gcgagcacct
     4141 cggcaccggc gagatccgca ggtggttcgg cgaggccgcg gcccggtcct tcgacgacac
     4201 ctcgggacgg ccggtcgtcc tcgcgtgcgg ccccgaggag ctgcacaccg ggggcctcga
     4261 ggcgttccgt gtcctgctcg tcgagcgagg tgtgcccgcg ctgttgctgg gcgcgcagac
     4321 gccgccggac tcgttcgcgc tgaccgtgcg cgactccggt gcggccgcgg ccgtgatgct
     4381 ctgccaccgg gcccaggcgc gccgcggctc gctcgtcacc ttgaggctgc tctcgtcgat
     4441 cccgaagacg aaggtgttct acgcgggcaa cggcttcgcg gccgcccgcg gacgccgtag
     4501 cgtcccgggg acgtatctcg ggacggacct gtcggcggcg gccgacctcg tcgcgggcgc
     4561 gatcgcctga gtcgctcggc cgcgacgtcc tcgtcacgcc ggggatgcga cacgatggcg
     4621 tggtgagcga cgcggtgatc gaagcgcaag gaatcgtccg gacctattcg tcgggggccg
     4681 aggccgtcca tgcgctgcgc ggcgtcgacc tcgccgtcat gcccggcgag ttcctcgcga
     4741 tcaccggggc ctcgggctcc ggcaagtcga cgctcctgca cgtgatcggc ggcctcgacc
     4801 gtcccgacga cggccgcgtg gtcctcgagg gccgcgacat gtcgtcgctg cccgacgagg
     4861 agctcgcgct cgtcaggcgc cggcatctcg gtttcgtgct ccagttcttc aacctcctgc
     4921 cgacgctgac cgcggtcgag aacgtcgcgt tcccgctgct cctcgacggg cacccggacg
     4981 ctctgccccg ggcgagcgag tcgctcgggt cggtcggcct gtcggagcgc gccgaccacc
     5041 gcccgtccca gctctccggc ggcgagcagc aacgggtcgc gctggcccgg gcgctcgtca
     5101 cgaagcccgc cgtcgtgctc gccgacgagc cgaccggcaa cctcgactcc gcgaccggcg
     5161 aagggatcct cgggctgctg cgcgccacgg cggaccgggg gcagacgatc gtgatggtga
     5221 cccacgacgg gcggactgcc gagtacgccg accggaccgt ccgcctcgca gacggaaggc
     5281 tgacgtgaag ggcctcctcg cgttcctgcg acgcgtcaac gggcgtcatc tcgtcgagaa
     5341 gcggctgcgc acggcactga ccatcggcgg cgtcgccgct ggggtcgcgc tcgtgacgtc
     5401 gatcaccgtg atcaacagca cgctgctctc gtcggtgcgc gagggcatca ggaacctcgc
     5461 gggcgcggcc gagatcgagg tcgcctcggc cgacgagacc ggcctgccgg aagacgtcgt
     5521 cgccgaggtc gctgccgtcg ggggcgtaga agccgcggtg ccggtggtgc gtacgaccgc
     5581 gcacctgacc gggccggccg gcgagtccga cacgatggtc ctcggcgtcg gccccgggtt
     5641 cctgaggctg ttcccgaccg ggctcggcga cgcggggcgc gtcgtggtcg agggcgagct
     5701 cgggttgagg gcgcgcggcg tcgtgctcgc ggaacaggtg gcggagaggc tcggcgcgga
     5761 gatcggcgac gtcgtgcgcg cggagacgcc gtcgggcagc gtcgcgctcc aggtgaccgc
     5821 acacgcgtcc ggccccggcc tctcgctgct caacggcggc gacgtcggga cgatgtcgct
     5881 cgaggccgcg caggaggcgt tcgagaagcg cggccgcgtc gattccgtct acgtcgtcgt
     5941 ggaagaggac gtcgcggccg ggcgcgtcga gagcgcgctc gacgacgtcc tcggcggcgc
     6001 ggccatcgtc ggcccgcccg gcgagagggg agaggcattc gagcgcacct tcgagagcat
     6061 cgccgcgctg acgtcgctcg ccggcgcggt ctcgctgttc gtcgcgctgt tcgtcgtcta
     6121 caacacgatg tcgatcgcgc tcgcggagcg caggcgcgag gtctcgatgg cgatcgcgct
     6181 cggcgcgaag cggtcgcagc tcttcgccgc gttcctcggc gaggccgggc tcctcgggac
     6241 ggtcgcgtcc gtagcggggg tcggagccgg gatcgtgctc gcgcgcgtcc tcgtcgggcg
     6301 cgccgtcgac cagttcgccg cggtcggcgt cagggcgacg ggccccctcg cggtcgagcc
     6361 gtggcacgtg ctgctcggcc tcggcgcggg catagcggtg tcgctcgccg gcgcgttcct
     6421 gcccgcacgc cggatcatgc gcgtcgcccc ggtcgaggcg ttgagaccgg aggcggggct
     6481 cgagtgggcg cggacgcggc ggggcgcgct gacgccggcg cgatcggtcg ctctcgcggt
     6541 cggcggggtg atcgtctgtc tcgggggcat ggcgctgtac atcgagaccg gcgcgtaccc
     6601 cgtagcggtc gtctcgctcg cggccgggct cggcggcgtc acgacgatcc ttcccgtcgc
     6661 agttccgctc ggtgtgcggg tcctccgccc ggtcttcacg cggctcttcg gcgcgaccgg
     6721 gcgcctcgcc gccgacgcac tggcgaagaa ccccggccgc acctcggtga cggtggggtc
     6781 gctcgtgctg acgctcggcc tcgtgatcgg ggtcggcggc gccatcggtt ccctcgaccg
     6841 ccagttccgc gacttcgcgc agcactggta cagccagccc ctgtacgtcc agacgaactc
     6901 gtacaccggg ggggtcgagt ccgaccagcc gctctcgggg aagttcgtcg ccgcgctcga
     6961 agaggtcgag ggcgtcgaca acgtgctgcc gttccggtac gcgaacctca ccgccgacgg
     7021 cgagcaggtc atcgcctacg tccatccgac gagcggcgag gcgcgcgccg acgcggtcga
     7081 gggactgtcg gtaggcgacg tcgactccga gccgctcgtc gacgcgctcg agcgcggcga
     7141 ggtcgtcgtc gccgagcaga tggcgcagcg gcgcgagctc gagatcggcg acacgctctc
     7201 gatcgcgggg ccccagggcc tgaaggactt caccgtcggc ggcttcttca acgacctcgc
     7261 gtcgttcgac tcgatgtaca tgtcggcgga cacgtaccgc gagcactgga acgacgaccg
     7321 cgcggactcg ttcggcgtcg acccggagcc gggcgtcgac gtcgcggcgc tcgagcgcag
     7381 gctcgaggag accgtcgagg cagagggcat gccggcgcag gtgatccgca aggaggagca
     7441 ggtcgagaac atcaccgcta ccgtcgacca gatcttctcg atcgccgagg gcatccagct
     7501 cgcggccgtg ctcgtcgcgt tcctgacgat cgtgaacacg atgttcaccg cggtcatcga
     7561 gcgccgctgg gagttcggct tgcagcaggc gatcgggatg gggcgccgtc agctagggcg
     7621 gacggtcgtg ctcgaggccg cggggatcgg cgtcgtcggc ggcgtcggcg ccgtcgtgct
     7681 cgggtccggg ctcggcgtgc tgatgctcgt cagcatgaac ttcctctacg cgttcgacat
     7741 cccctaccag cctccgtgga ccttgttcgc cgtcgcgatc gcggcgggga ccctgatcgc
     7801 ggcgctgtcg gcggtgtacc cgcgccggct ggccacgcgc cttcggatcg tcgagtgcct
     7861 ccgctacgag taggggggtc gcgcccccgc cggctgctcg gggtgctgct cgccggatgc
     7921 ttcggcgccg cggcctcgta cgccgcgctc gggctcctcc cggccgtcag gcacgaggcc
     7981 ggccccgcga ccctggacac gacgctcgcg ccgggagggg gtcgcatggt cttgcgaatc
     8041 ccgccgctcg ggaccgtgac ggccgggacg cacacgcctc cggtcgcgct gcgcatgacc
     8101 ttgtccgagg tcgacgtccc gcgcctgacc gagcgggtgg gcaacgccgc ggcccgggcg
     8161 gagctagccg ccgccgtcga ggaagacctc cgcgctctcg tcccccgcct cgtttggcag
     8221 acgtgcctcg gcgccgcggt tctcggcggg atcgtggccg cgctgttgcc gcgccgcagg
     8281 ctctcctacg tcgcggcggg ggccgcgggc ggcgccgtcg cggcagcggc gctcctcggc
     8341 ctgaccgcgg cgtcgttcga cgtagacgcg ttcgagcagc cgagcttcac cggcgcgctc
     8401 gtccgggcgc cggtcgtgat cgacgctctg cagagcggcg aggtgacgct cggcgccatc
     8461 cagtcccgtt acgagaccgc ggcggaccgg ctgtcggagc tgctggcgct cgtcgccgag
     8521 ccgggccagg accccgccgg cgactcggtc gcgatcctgc acgtgagcga catccactcg
     8581 aacccgctgg gagtccagct cgcgcgccgc cttgccgagc gcttcgacgt cgacgccgtc
     8641 ctcgacaccg gtgacctcac gaacttcggc gtgacgctcg agtcgcgcat cgggcagctc
     8701 gtcgagcgca tcgacgtccc ctactacctc gtgcccggca accacgactc cgcggcgaac
     8761 gaggccgcgc tcgaccgcgt cgagaacctc acgatcctgg acgggacggt cgccgacgtg
     8821 tccggcgtga gcatcctcgg ctggggtgat ccaaccgaca cgaactggaa caacatctcg
     8881 ccggacgaag ccgcggagat ccgcgtggag gagggtgagg acgcggccgc cgaggtcgcc
     8941 cgcgtcgcgc cggacgtgct cgcggtccac gacagccgga tggccgaggc ttcctacggc
     9001 gcggtcccgc tcgtgctctc tggccactac caccgccgga tcgtggagga ggaagacggc
     9061 acgaccgtcc tcggcgtggg atccaccggc gcgaccgggc tgaagttctt cgtggaggcc
     9121 gcgcgcgact acgaagccga gatcctctac ttccgtgacg gcgtcgcggt cgccgtggac
     9181 tacgtgcggt tcagcggcct cggcacggag ttcgagatcg agcgcgacgc cctcgacgag
     9241 tgaccggacg cgggaaggag ccctcgacgc ggccggttgt cgggagcatg gaagcctcgt
     9301 tgaccgtgcg ctcggtccgt ctcgtcgccg ggctgtggct gttcgccctc ggcacggtgc
     9361 tgacgctgcg ggcgagcctc ggcgtgcccc cgtgggacgt gctccacgac gggatccggc
     9421 gcaacacccc gctgagcttc ggcatcgccg tcatcgcgat cggtctcctg ctcgtgctcg
     9481 tcagcgcgct gttcggcatc aggccgggcc ccgggacgat cgcgaacatg ctcctggtcg
     9541 gcacgttcgt cgacctgatg ctgctgagcg acgccgggac ggggctcgag gacgcgcaca
     9601 tggcgtggcg ggtcgcggtg gccttcgccg gggtcgcggt cgtcgggctc ggctccgccc
     9661 tgtacatcgg cgcgaacctg ggggccgggc cgcgcgacag cctgatggtg ctcgcggcga
     9721 cgaagctcgg cgtgcgcgtc ggcgtcgccc gcgcggtgat cgagggcacg gccctcctcg
     9781 tcggatggct cctcggcggg gccgtcggca tcggcacgct tctcttcgtc gtcacgatcg
     9841 gtccctcggt ggacgtcttc ttccgtctct tccgcatgga ggccgacgga cggagggcgg
     9901 gcccggtcgc ccttgacacc tccgcctgac taacgcgata gtgctccaaa tcccgttaga
     9961 aaggagctgc gcgttggacg agcagggcag ggtggtcgag ctggcgatct tcaagctgaa
    10021 ggagggcgtg ggccgcgacc agctgatgga gacggtcgac gccgtgaccg aatgggcgaa
    10081 gcgccagccg gggttcgtgt cgcgcgacct cacttactcg gccgagagcg acacctgggt
    10141 cgacgtcgtc tggtgggaga gcatcgagtc ggcccacgcg gcctcggagg ccgccatgac
    10201 gtccgagtcg tgcgcgccga tgttcgggct gatcgacctc gaggggaccc agatgatcca
    10261 cggcgagcgc gtcgccgctt aggaggacgg ggccgcggtg ctgcaggtga cgtgggagtg
    10321 gctgggcatg ccgcccgcgc tcgacctggc gaacaccgtg gcgatcacgg agggcgccga
    10381 gcaggacctc gtcggctcga acgaggactt cgaggagtgg gcccggatcg agtcgagctt
    10441 cgtcccggcc cccgtcggtc tgctgaggct ccaccggagc gagctgatgg aagtgcgggc
    10501 ggcggtcagg gacctcctgg ccgccgtcac cgcgcacgag ccaccgccgc gccgagcgat
    10561 cgagacgctc aaccggctga gccgcgcggc ccccgagtgg accgagctcg acgccgcgtc
    10621 gtggagagtc gtcacccgga ccggcgcggg ggccgcg
//
LOCUS       DASQDO010000532         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1095980, whole genome shotgun sequence.
ACCESSION   DASQDO010000532 DASQDO010000000
VERSION     DASQDO010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095980"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..636)
                     /locus_tag="VEV43_14645"
     CDS             complement(130..636)
                     /locus_tag="VEV43_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24804.1"
                     /translation="MNDEIKGWFAGRLPDDWFEAAPEVTGDRDEVLVVGRLGDVDLGA
                     GASEDGTNAARLARIKRFREDTRSARMAIADEAERLFGRKVAWGAECGGVRRVFTNMA
                     IPVMTRLRLRERTVLDTLIDAGVARSRSEALAWCVRLVGKNEAEWIASLLDALTEVEK
                     ARAQGPAA"
     gene            complement(726..2576)
                     /locus_tag="VEV43_14650"
     CDS             complement(726..2576)
                     /locus_tag="VEV43_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24805.1"
                     /translation="MAAGDFALVLAQLFLSVLIGWAVAWALTRWARLDELAVVERAAL
                     AIAGFLAFAVAAMLLNVVTRGLVFGTPGVVPALAAVPVLWWWRRSRERLPRPSRAWVA
                     PAALALVLVAIYLLPVLLTGSGVRTGDPPWHLGWTEQLLGGEPVPVGPAPEVARNAYP
                     WGFHAFLATMVRLVPASTPLLAHETAHVILLAGIPLVAACLARRVNRRAGLAAAACTS
                     LIGGFGWLMAREPAFVTSPREARFGADLVVASPNSVYELMPPALPRELGLLVAGVAAL
                     LLLVALEHGTRRARVLAGVLAGLVGVVSVPMLSTALIWTIVACLFVGGTKWRAAAEVF
                     VPAVAVFATWAAPVGLGYLRYGGFVDITPRLGMEWPLPTALGSYGLLLPLAIGGIAGA
                     RSTREGRVLLSLTAASAALLGLAVARDVFDWTVWNNATLLHQGRMWPPVHLLAGVFGG
                     VALERAHAAVARRSARVAAVAAGAVLCVAAASPVLASIRLAQILSDGSAGFEYAGRDF
                     ESGSFVREAGELLSPDDVVSVTGSDDLAWALFQTSGARLARYDDSRFERNDLRIRFAD
                     LAAEWDERVGGRGFTPSHTVVPLGASGPVLGDQLVIGEFRGERWVLTELD"
     gene            complement(2576..4282)
                     /locus_tag="VEV43_14655"
     CDS             complement(2576..4282)
                     /locus_tag="VEV43_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYP24806.1"
                     /translation="MFRRILIAHRGEIAVRIARTCRALGVEAVAVHSDVDAGARHVAA
                     ATRAVHLPGVAPTETYLNVEAILEAARSTGAEAIHPGYGFLSESASFADAVVGAGVVW
                     VGPPPAAIRAVGDKISARRLAVEAGVPVVPGITDPVEDVAVLEQFASEHGYPVAIKAS
                     GGGGGRGLKVAHSAAELEPAWTSARREAEAYFGSHDVFVERYLEGPKHLEVQLLAPGP
                     DEALWLGVRDCSLQRRHQTLIEETPPPRWAERVPEMGEAAVALSKACGYVNAGTVEML
                     VDRAGEFHFLEVNARLQVEHTVTEEVLGLDLVECQLRIASGERLDFSQADLVPRGHAI
                     ECRINAEDPARGFAPTPGRLVRYVEPAGPGVRVDSGYVEGDEVPGAYDSLIAKLVTHG
                     ADRDEARERMLRALDEYTVEGIETTIAAHVLLLQEESFVAGTHDTTTVEFGDVLDALA
                     ASEEELRDVLLVGGKPVRLWNPAMAASAAAATHGAVAGGDVVSPMQGTILKVLVSVGD
                     EVEAGQPLVVLEAMKMENEIASPAAGVVEAVDAVVGETAGAGAVLVRVGSNGRTEPEP
                     SP"
     gene            complement(4282..>4624)
                     /locus_tag="VEV43_14660"
     CDS             complement(4282..>4624)
                     /locus_tag="VEV43_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYP24807.1"
                     /translation="SALREELPGLTLNMHFHDTRGTGLANVLTALDLGIAYFDASVGG
                     MGGSPYAAGATGNVATEDLVHMLADMEIETGVDLDKLLDAARIAQGFIQGELPSKVLK
                     AGPRWAVVGRA"
BASE COUNT          722 a         1741 c         1592 g          569 t
ORIGIN      
        1 tgccgttgcc ggagacgcgg cggccgccgc ggaccggctc gtagaccgcg aggttcgtca
       61 ccgagggcgc gagccgttcc gcgatccgcg tgacgatgct cgagtacgcg tcgagcgccg
      121 cctcttcgct cacgccgccg gtccctgcgc gcgcgccttc tcgacctccg tgagcgcgtc
      181 gagcaacgac gcgatccact cggcctcgtt cttgccgacg agccggacgc accacgcaag
      241 cgcctcgctg cgactgcgcg ccacgccggc gtcgatcaac gtgtcgagca ccgtccgctc
      301 gcgcagacgg agccgcgtca tcaccgggat cgccatgttc gtgaacactc gccggacgcc
      361 gccgcactcg gccccccaag cgaccttccg gccgaacagc cgctcggcct cgtcggcgat
      421 agccatgcgt gccgatcgag tgtcctcgcg gaacctcttg atgcgcgcaa gccgcgcggc
      481 gttcgtcccg tcctccgagg cgccggcgcc gaggtcgacg tcgccgagcc gcccgaccac
      541 gaggacctcg tcccggtcgc cggtgacctc gggggcggcc tcgaaccagt cgtccggcag
      601 gcgtcccgcg aaccagccct tgatctcgtc gttcatccgt cctcctgcta gtgggattac
      661 gagattacac ggcaagtcag cttacggagg actcgaggac tcgacggcct gacgcccccg
      721 cggcgctagt cgagctccgt caggacccac cgctcgcccc ggaactcgcc gatcaccagc
      781 tggtcgccga gcacggggcc ggatgctccg agcggcacga ccgtgtgcga cggcgtgaag
      841 ccgcggcccc cgactcgctc gtcccactcc gccgccaggt cggcgaagcg gatccggagg
      901 tcgttgcgct cgaagcgcga gtcgtcgtag cgcgccagcc gggcgcccga cgtctggaac
      961 agggcccacg cgagatcgtc cgaccccgtc accgagacga cgtcgtccgg cgagaggagc
     1021 tcgcccgcct cgcgtacgaa cgagccgctc tcgaagtcgc ggccggcgta ctcgaacccg
     1081 gcgctgccgt cggagaggat ctgcgcgagg cggatggacg cgagcaccgg tgacgccgcc
     1141 gcgacgcaca gcacggcgcc ggccgcgacg gcagcgaccc gggcggaacg tcgcgccacg
     1201 gcggcgtggg cgcgctccag cgccacgccc ccgaagaccc ccgcgagcag gtggacgggc
     1261 ggccacatcc gtccctgatg aaggagcgtc gcgttgttcc aaacggtcca gtcgaagacg
     1321 tccctcgcga cggcaagacc cagcagcgcc gcgctggcgg ccgtcagcga cagcaggacg
     1381 cggccctcgc gcgtgctccg cgcacctgcg atccccccga tagccagcgg caacaggagg
     1441 ccgtaggacc cgagcgcggt cggcagcggc cactccattc cgaggcgcgg agtgatgtcg
     1501 acgaaccctc cgtagcgcaa gtagccgagg ccgacgggag ccgcccacgt cgcgaacacc
     1561 gctacggccg gcacgaagac ctctgcggcg gcgcgccact tagtgcctcc gacgaacagg
     1621 caggcgacga tcgtccagat cagcgcggtc gacagcatcg ggacgctcac caccccgacc
     1681 aggccggcga gtacacctgc cagcaccctg gcgcggcgcg tcccgtgctc gagcgcgacg
     1741 agcaggagta gtgcggcgac gccggcgacg aggagcccga gctcgcgcgg cagcgccggg
     1801 ggcatgagct cgtagacgga gttgggggac gcaacgacga ggtccgcgcc gaagcgcgcc
     1861 tcccgagggc tcgtcacgaa cgcgggctcc cgtgccatca gccagccgaa accgccgatc
     1921 aacgaggtgc aggccgccgc ggcgagacct gctcgccggt tcacgcgccg cgccaggcac
     1981 gccgcgacga gagggatccc cgccagcagg atcacgtgcg cggtctcgtg cgcgaggagc
     2041 ggtgtcgacg cggggacgag acgcaccatg gtcgcgagga aggcgtggaa gccccacggg
     2101 taggcgttgc gcgcgacctc gggggccggg ccgacgggga cgggctcgcc tccgagcagc
     2161 tgctcggtcc acccgagatg ccacggcggg tcgccggttc tgacgcccga acccgtcaag
     2221 agcaccggga gcagatagat cgcgacgagc acgagcgcca gcgcggcagg cgcgacccac
     2281 gcacgcgacg gccgcggaag gcgttcccga gaccgtcgcc accaccacag cacagggacc
     2341 gccgcgagcg cgggaaccac cccgggcgta ccgaacacga gcccccgcgt gacgacgttc
     2401 agcagcatcg ccgcgaccgc gaacgcgagg aacccggcga tcgcgagcgc cgctcgttcg
     2461 acgaccgcga gctcgtccaa ccgggcccag cgcgtcaacg cccacgcgac ggcccatccg
     2521 atgagaaccg aaagaaagag ctgagccagg acgagcgcga aatcgcccgc ggccactacg
     2581 gcgagggttc cggctcagtc cgcccgttcg aaccgacgcg cacgagcacc gctcccgcgc
     2641 ccgccgtctc ccccaccacg gcgtcgaccg cctcgaccac acccgcggcg ggagaggcga
     2701 tctcgttctc catcttcatc gcctcgagca cgaccagggg ctgtccggcc tcgacctcgt
     2761 cgcccaccga gaccagcacc ttgaggatcg tcccctgcat cggcgagacg acgtcgccgc
     2821 ccgcgacggc accgtgggtc gcggcggcgg ccgacgcggc catcgccggg ttccacagcc
     2881 gcactggctt cccaccgacg aggaggacgt cgcgcagctc ctcttctgaa gccgcgagcg
     2941 cgtcgaggac gtcgccgaac tcgaccgtcg tcgtgtcgtg cgtccccgcg acgaacgact
     3001 cctcctgcag gagcagcacg tgcgcggcga tcgtcgtctc gatgccctcg accgtgtact
     3061 cgtccagagc gcgcagcatc cgttcccgtg cctcgtcccg gtcggcaccg tgcgtcacga
     3121 gcttcgcgat gagcgagtcg tacgcgccgg ggacctcgtc gccctcgacg tagccggagt
     3181 cgacgcgcac tcccggaccg gccggctcga cgtagcggac gaggcggccg ggcgtcggcg
     3241 cgaagccgcg cgcggggtcc tcggcgttga tgcggcactc gatcgcgtgg ccgcgcggga
     3301 cgaggtcggc ctgcgagaag tcgagcctct cgcccgacgc gatgcgtagc tggcactcga
     3361 cgaggtcgag cccgaggacc tcctcggtga ccgtgtgctc tacctggaga cgcgcgttga
     3421 cctcgaggaa gtggaactcg cctgcgcggt cgacgagcat ctcgaccgtg cctgcgttga
     3481 cgtagccgca cgccttcgag agcgcgaccg cggcttcgcc catctccggc acccgttcgg
     3541 cccagcgcgg cggaggggtc tcctcgatca gcgtctggtg ccggcgttgc agagagcaat
     3601 ctcggactcc gagccacagc gcctcgtcgg ggcccggggc gaggagctgc acctcgaggt
     3661 gcttggggcc ttcgaggtag cgctcgacga agacgtcgtg cgagccgaag tacgcctcgg
     3721 cttcacggcg cgcggaggtc catgccggtt cgagctcggc ggcggagtgc gcaaccttca
     3781 ggccgcggcc ccctcccccg cccgacgcct tgatcgccac gggatagccg tgctccgacg
     3841 cgaactgctc gagcacggcg acgtcctcga ccggatcggt gatccccggc acgaccggca
     3901 cgccggcttc gaccgcgagc cgccgagccg aaatcttgtc gcccacggcg cggatcgcgg
     3961 cgggcggcgg accgacccag acgacccctg cgccgaccac ggcgtccgcg aacgaggcgc
     4021 tctccgacag gaagccgtag cccgggtgga tcgcctcggc ccccgtggag cgagccgctt
     4081 cgaggatcgc ctcgacgttc aaataggtct cggtgggagc aacgcccggg aggtggaccg
     4141 ctcgggtcgc ggcggcgacg tggcgcgccc ccgcgtcgac gtcagagtgc accgcgaccg
     4201 cttcgacacc gagcgcgcgg cacgtacgcg ctatccggac cgcgatctca ccgcggtgcg
     4261 cgatcaggat ccgccgaaac atcaggcccg ccccacgacg gcccagcgcg gccccgcctt
     4321 caggaccttg ctcggaagct caccctggat gaacccctgc gcgatgcggg ccgcgtccag
     4381 cagcttgtcg aggtcgacgc cggtctcgat ctccatgtcg gcaagcatat ggaccaggtc
     4441 ctcggtcgcg acgttgccgg tggcgccggc cgcgtacggg ctgcccccca tgcccccgac
     4501 cgacgcgtcg aagtaggcga tgccgaggtc gagcgccgtg agcacgttgg cgagccccgt
     4561 gcccctcgtg tcgtggaagt gcatgttgag cgtgaggccg ggcagctcct cgcgcagagc
     4621 gctc
//
LOCUS       DASQDO010000533         1757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_850817, whole genome shotgun sequence.
ACCESSION   DASQDO010000533 DASQDO010000000
VERSION     DASQDO010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850817"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VEV43_14665"
     CDS             complement(<1..754)
                     /locus_tag="VEV43_14665"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYP24808.1"
                     /translation="MTPGPVLAHGEAPAAGLLTFDPLVWAGIAGAAWLYFRAVRRTRG
                     WPRHRSWCAGLGLGALFVATSGPPSVYEGSVFWAHMVQHLLVTLVAAPLLAFSAPAAL
                     LLRAGSARARDALLRIVHAGPVRALTHPVVTWAGFGLVTWLTHYSPLYEAAVHDELLH
                     ALEHALYLGSALLFWAPVAGLDPTRRVARPVRVAYLLAAIPVQSFLGLAIYSADAPLY
                     DHYASLDDQRTAGAVMWIGGDVVLLGWTGVAAA"
     gene            complement(774..1448)
                     /locus_tag="VEV43_14670"
     CDS             complement(774..1448)
                     /locus_tag="VEV43_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYP24809.1"
                     /translation="MSTQDDSLRPDLTTEVRVKVPLPLVIPLVSLAVIGLVAYGFAQV
                     LLAVPKEAATMLAMVAAANVMGAFAYAALRPRMQPVTMAELGVIVLYPVIIGIAIAQF
                     GFGAEAHGHDTAAGETHGAGAAGEGAAPSGPTTAVVATGLVFDVETLVLEAKKDTSIE
                     FTNEDSAPHNIAIYEDDSASGEAIFEGEQISASSTTYEFKAPPAGEYYFQCDVHPDMN
                     GEVLSE"
     gene            complement(1453..>1757)
                     /locus_tag="VEV43_14675"
     CDS             complement(1453..>1757)
                     /locus_tag="VEV43_14675"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYP24810.1"
                     /translation="GKMLYLFGQSILPTVPASFLTFATEPIYRAYEEFPRLWGIGVVT
                     DQRIAGLIMKVGGGLLLWSIIAVMFFRWSSAQERQEIPEITWDDFERELEAWELRK"
BASE COUNT          291 a          626 c          616 g          224 t
ORIGIN      
        1 cggcggccgc cacgcccgtc cagccgagca gcacgacgtc gccgccgatc cacatcacgg
       61 ccccggcggt gcgctggtcg tcgagcgagg cgtagtggtc gtacagcggc gcgtcggccg
      121 agtagatcgc cagccccaga aacgactgga ccgggatcgc ggcgagcagg tacgccaccc
      181 gcacgggccg cgccacccgc cgcgtcgggt cgagccccgc cacgggggcc cagaacagca
      241 acgcggagcc gaggtagagc gcgtgctcga gcgcgtgcag cagctcgtcg tggaccgccg
      301 cttcgtacaa gggcgagtag tgcgtcagcc acgtcacgag cccgaagccc gcccacgtca
      361 cgacgggatg ggtcagcgcc cggacgggcc cggcatggac gatccgcagc agcgcgtcgc
      421 gcgcccgggc cgaacccgcc cgcagcaaca gcgcggccgg cgcggagaac gcgagcagag
      481 gcgcggcgac gagcgtgacg agcaggtgct gcaccatgtg ggcccagaac acggacccct
      541 cgtagacgga ggggggaccg ctcgtggcga cgaacagggc gccgaggccg agcccggcgc
      601 accacgaacg gtgccgcggc cagcccctgg tccggcggac ggcgcggaag tagagccatg
      661 cggcgccggc gatgccggcc cacacgaggg gatcgaacgt gagcagtccc gcggcgggag
      721 cctcgccgtg cgcgaggacg gggccgggcg tcacagcgcc cgcggccgga cgactactcg
      781 gagagaacct ctccgttcat gtcgggatgg acgtcgcact ggaagtagta ctcaccggcg
      841 ggcggagcct tgaactcgta ggtcgtgctg ctcgccgaga tctgctcgcc ctcgaagatc
      901 gcctcgccgc tcgcgctgtc gtcctcgtag atcgcgatgt tgtggggggc cgagtcctcg
      961 ttcgtgaact cgatcgacgt gtccttcttc gcctcgagca cgagcgtctc gacgtcgaac
     1021 acgagcccgg tcgccaccac ggcggtcgtc gggcccgacg gcgcggcccc ctcgccggcg
     1081 gccccagccc cgtgggtctc gccggccgcg gtgtcgtgcc cgtgcgcctc cgcgccgaag
     1141 ccgaactgcg cgatcgcgat gccgatgatc accgggtaca ggacgatgac gccgagctcg
     1201 gccatcgtca cgggctgcat gcgcgggcgc agcgccgcgt aggcgaaggc gcccatcacg
     1261 ttcgcagcgg cgaccatcgc gagcatcgtg gcggcttcct tcggcaccgc gagcagcacc
     1321 tgagcaaacc cgtacgcgac gagcccgatc acggctagcg acaccagcgg gatgacgagc
     1381 gggagtggga cctttacgcg gacctcggtg gtgaggtccg gccgaagcga gtcgtcctga
     1441 gtgctcattg cctcacttcc tcagctccca agcctccagc tcccgttcga agtcgtccca
     1501 ggtgatctcg gggatctcct ggcgttcctg cgcgctgctc caccggaaga acatgaccgc
     1561 gatgatcgac cacagtagca gtccaccccc caccttcatg atcagcccgg cgatcctctg
     1621 gtcggtcacc acgccaatgc cccacaggcg cgggaactcc tcgtaggcgc ggtagatcgg
     1681 ctcggtggcg aacgtcagga acgaagccgg gaccgtcggg aggatggact gcccgaacag
     1741 gtagagcatc ttcccgg
//
LOCUS       DASQDO010000534         5923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_483953, whole genome shotgun sequence.
ACCESSION   DASQDO010000534 DASQDO010000000
VERSION     DASQDO010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483953"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..697
                     /locus_tag="VEV43_14680"
     CDS             80..697
                     /locus_tag="VEV43_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HYP24811.1"
                     /translation="MRLRLIPREESFFDLFEEMAHKVHQGAEGLLDLLRDYRDLDIKA
                     GRVVDIEHEGDEITHEMIRRLNTTFVTPFDREDIHRLASNLDDVLDHVEAAAEYLQLH
                     KIENPLPQMVALAEILQQAAVITATAMPGLRKMKGLDDYWVEINRLENDGDRAYRRTI
                     AELFSGDYKAMDVLKFKDIIEEIEQAIDRLEDVANTIESIVMKQG"
     gene            699..1811
                     /locus_tag="VEV43_14685"
     CDS             699..1811
                     /locus_tag="VEV43_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HYP24812.1"
                     /translation="MEDQVSLWLVLALAYLFEFSNGWTDAPNSIATVVSTRVMRPFQA
                     VAMAGVLNLLGALSGTAVAKTIASGIVDADFVSLNTIAAAVLAATVWALGAQYLGLPS
                     SESHALVAGLLGAGYAAGGFPALEWEGTRKSLIGLVTSPVGGFFVALLLMVAIYRIFA
                     KTRPAIVRRIFGKAQIGSAAFMAFSHGANDAQKTMGIIALAIVLDKAGTGASAPPEFE
                     IDFWIVITAAVVMGLGTMVGGWRVVKTIGLRLTKLEPVHGFAAETGAAAVIFGAAQVG
                     IPVSTTHAIGSAIMGVGATERLSAVRWGVAQQVVTAWILTWPSCALLGYALESSFKRL
                     QPEVVIVAVIGVATALWTRGTLRRQKEEAARFEAPA"
     gene            1808..2125
                     /locus_tag="VEV43_14690"
     CDS             1808..2125
                     /locus_tag="VEV43_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24813.1"
                     /translation="MTPEDQELQRLRKQAKDDRKLIADAAQVLGEIDRGPGLSAEHAD
                     VLAALRIRLEGKPRAKLEDLLSAAGDISAKKDLADVLGDTGDEPKSDWPVIEEKKRDW
                     PGL"
     gene            complement(2141..3019)
                     /locus_tag="VEV43_14695"
     CDS             complement(2141..3019)
                     /locus_tag="VEV43_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008376196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HYP24814.1"
                     /translation="MTDDALVQHSEPELNRPVMVAAFRGWNDAGDAASFAAEHLQRVW
                     GGEQLASIDPETFFDFQDTRPQVELVDGVTRKIAWPANEFSAARLRGAPHDVIVLVGT
                     EPNLRWKSFASLVVSLARRHDVQLVITLGALLADVPHSRPVPIKGTAVDQELIDRLGL
                     ERSRYEGPTGIVGVLHDSLAQAGIASASLWAAVPHYLAVTPNPKAALALVDKAVGLVG
                     WPAEIDDLERASAAYEERVSEMVSSDEDVTAYVKLLEDRTDERNRESLEEMELPSGDA
                     LAAELETFLRNRGAED"
     gene            complement(3060..3707)
                     /gene="sigH"
                     /locus_tag="VEV43_14700"
     CDS             complement(3060..3707)
                     /gene="sigH"
                     /locus_tag="VEV43_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HYP24815.1"
                     /translation="MSVWTEERQDLDGLEDLDLVKRVRDGDERCLETLLSRYRNFARS
                     KARSYFLAGSDKEDVVQEGMIGLFKAIRDFDLSQDTPFRAFAELCISRQILTAIKTAN
                     RQKHQPLNSSVSLDAPAYGDEKSESSVGDNLVATTLSDPAELVISAQEIEAIRDSMRD
                     NLTELEGDVLRLYMDGKSYEEIAGALGNHVKSIDNALQRIKRKLQRHLDSRDAVA"
     gene            complement(3973..>5923)
                     /locus_tag="VEV43_14705"
     CDS             complement(3973..>5923)
                     /locus_tag="VEV43_14705"
                     /inference="COORDINATES: protein motif:HMM:NF020698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP24816.1"
                     /translation="WGPGRLDCTPETDMNPTGTSHQVACTVRDGNNQTASGANVDVEA
                     TGANDPDGGNTPQTPDFTCTTDGNGGCSFTHGGTGGGSTAGAGTTTYRAWVDLDGNNA
                     TPEADVGENLDEGGNGAGSTNEPDHTDVVQKVWTAGALDCTPETAQNPTGTSHTITCT
                     ARDSSGNVVPGPEVDAEVTGANDRDSSGDLTNPDFSCTTSAAGSCTFTHGGGGNNGSS
                     DATGTSTYRSWIDADSSNATAEADATESRDESGAGAGGTTDPDATDVVEKTWVTQPRT
                     ISCTPATATNPAGTQHTVTCTVRDRSGQPLAGRAVTLSSTGTGTLSSQSQQTTNNAGQ
                     VQATTTSTEEGTQSITATLGDDLQGNEPNDVDECDRAAGDPAGSPAGQCSATVTKTWI
                     APLAQAANLTVTPAEATNEVGDDHTLTATVTDANGSPMEGAPVTWTSTGVGEIEESDA
                     TTDANGVAMAVISSEEAGDQLVSVSTSPCAQGGDCTDTALKHWTTGPCTITGTEAGER
                     LTGTNGDDVICGLGGNDTINGRGGNDVILGQGGNDRMSGNGGRDRLRGGGGADIVVGG
                     AGRDTVGGSRGNDAVRGSGGPDVVLGNGGKDRVSGGAGNDRVSGGGGNDRVSGDRGND
                     RLDGGRGFDRCNGGPGRDDEQGCER"
BASE COUNT          814 a         2040 c         2037 g         1032 t
ORIGIN      
        1 gtagacaatc tgtagatagc gagaggttgc ctacgggcgc tatcctgccc ggcgacagcc
       61 ggccgacagg ggagcgtaga tgcgcctgcg tttgatcccg cgcgaggagt ctttcttcga
      121 cctgttcgaa gagatggcgc acaaggtcca tcaaggagcc gaagggctgc tggaccttct
      181 gcgcgactac agggacttag acatcaaagc cggacgtgtg gtcgacatcg agcacgaggg
      241 tgacgagatc acccacgaga tgatccggcg gctgaacacg acgttcgtga cgccgttcga
      301 ccgcgaggac atccaccggc ttgcgtccaa cctcgacgac gtcctggatc atgtcgaggc
      361 cgccgcggag tacctgcagc tccacaagat cgagaaccca ctcccgcaga tggtcgcgct
      421 cgccgaaatc ctccagcaag ccgcggtgat cacggccacc gcgatgcccg ggctgcgcaa
      481 gatgaagggg ctcgacgact actgggtcga gatcaaccgg ctagagaacg acggcgaccg
      541 cgcgtaccgc cgtacgatcg cggagctctt ctcgggtgac tacaaggcca tggacgtcct
      601 gaagttcaag gacatcatcg aagagatcga gcaggctatc gaccggctcg aggacgtggc
      661 caacaccatc gagagcatcg tgatgaagca gggctgaggt ggaggaccag gtatcactct
      721 ggctggtcct cgcgctggcc tacctcttcg agttctccaa cggctggacc gacgctccca
      781 actccatcgc cacggtggtc tccacccgag tcatgcgccc gttccaggcc gtcgccatgg
      841 ccggcgtgct gaacctcctc ggcgcgttgt cgggaacggc ggtcgcgaag acgatcgcgt
      901 ccggcatcgt cgacgcggac ttcgtctccc tgaacacgat cgccgccgcc gtgctcgccg
      961 ccaccgtgtg ggcgctgggc gcccagtacc tcggactgcc ctccagcgag tcgcacgccc
     1021 tcgtcgcggg tttgctcggt gcgggttacg cagccggggg cttcccggcg ctcgaatggg
     1081 aggggacgag gaagtccctc ataggtctcg tcacgtcgcc cgtaggtggg ttcttcgtgg
     1141 ccctcttgct gatggttgcg atctaccgga ttttcgccaa gacgcgtccc gccatcgtgc
     1201 ggcggatctt cgggaaggcc cagatcggtt cggccgcgtt catggccttc agccacggag
     1261 cgaacgacgc acagaagacg atgggcatca tcgccctcgc aatcgtgctg gacaaggcgg
     1321 gcacgggcgc ctccgcgccg ccggagttcg agatcgactt ctggatcgtc atcaccgcgg
     1381 cggtcgtgat gggcctcggc acgatggtcg gcgggtggcg ggtggtgaag acgatcggat
     1441 tgcggctgac gaagctggag cccgtgcacg ggttcgctgc cgagaccggg gccgccgctg
     1501 tgatcttcgg cgccgcgcag gtcggcatcc cggtctcgac gacgcatgcg atcggctccg
     1561 cgatcatggg ggtgggtgcc accgaacgcc tctccgccgt ccgttggggc gtcgctcagc
     1621 aggtcgtcac cgcgtggatc ctgacgtggc cgtcgtgtgc gctgctcggc tacgcgctcg
     1681 agtcctcgtt caagaggctt cagccggagg tcgtcatcgt cgccgtcata ggtgtcgcga
     1741 ccgctctgtg gactcgcggg acgctgcgtc gccagaagga agaggcggcg cgattcgagg
     1801 ctcccgcatg acgccggagg accaggagct ccagcgactg cgcaagcagg cgaaggacga
     1861 ccgcaagctg atcgccgacg ccgcccaggt cctcggcgag atcgatcggg ggccgggcct
     1921 ctccgccgag cacgccgacg tcctcgcggc gttgcggatc cgcctggagg gcaagccgcg
     1981 ggccaagctc gaggacctgc tgtcggccgc cggcgacatc tccgcgaaga aggacctggc
     2041 cgacgtcctg ggcgataccg gcgacgagcc gaagagcgac tggccggtga tcgaggagaa
     2101 gaagagggac tggcccgggc tgtagcccgg tcgatcgtcg ctagtcctcg gccccccggt
     2161 tgcgcaggaa cgtctcgagc tccgccgcca gcgcgtcgcc cgaaggcagc tccatctcct
     2221 ccagcgattc ccggttgcgc tcgtccgtgc ggtcttcgag gagcttgacg taggccgtca
     2281 cgtcctcgtc cgacgacacc atctcggaca ccctctcctc gtacgccgcc gacgcgcgct
     2341 cgaggtcgtc gatctctgcc ggccacccga cgagcccgac cgccttgtcc acgagcgcga
     2401 gcgcggcctt cgggttcggc gtcaccgcga ggtagtgggg cactgcggcc cacaggctcg
     2461 ccgacgcgat gccggcctgc gcgagcgagt cgtggagcac gccgacgatc cccgtcgggc
     2521 cctcgtagcg cgagcgctcg aggcccagcc ggtcgatcag ctcctggtcg acggccgtcc
     2581 ccttgatcgg gaccggccgc gagtgcggca cgtccgcgag caacgccccc agcgtgatca
     2641 cgagctgcac gtcgtgccgg cgagcgagcg agaccacgag cgaggcgaac gacttccacc
     2701 ggaggttcgg ctcggtcccc acgagcacga tcacgtcgtg cggtgcaccc cgcaggcgcg
     2761 cggcgctgaa ctcgttggcc ggccacgcga tcttccgggt cacgccgtcg acgagctcta
     2821 cctgcgggcg cgtgtcctgg aagtcgaaga acgtctcggg atcgatcgaa gcgagctgct
     2881 cgccgcccca cacgcgctgg aggtgctcgg ctgcgaagga cgccgcgtcg cccgcgtcgt
     2941 tccagccgcg gaacgcggcg accatcacgg gacggttgag ctccggctcc gagtgctgca
     3001 cgagcgcgtc gtcggtcatc gggccaggct actgccgggc cggaggaaag cggtgaaagc
     3061 taagcgactg cgtcgcgcga atcgaggtgg cgctggagct tgcgcttgat gcgctgcagg
     3121 gcgttgtcga tcgacttgac gtggttgccg agagcgccgg cgatctcctc gtaggacttg
     3181 ccgtccatgt agaggcgcag gacgtcaccc tcgagctcgg tcaggttgtc ccgcatcgag
     3241 tcccggatcg cctcgatctc ctgcgcggag atcacgagct cggccgggtc ggagagcgtg
     3301 gtggcgacca ggttgtcgcc gacggaggac tcgctcttct cgtcgccgta cgccggcgcg
     3361 tcgagcgaga cggacgagtt cagcggctgg tgcttctggc ggttcgcggt cttgatggcg
     3421 gtgaggatct ggcgggagat gcagagctcg gcgaaggccc ggaacggcgt gtcctgcgag
     3481 aggtcgaagt ccctgatcgc cttgaacagg ccgatcatgc cctcctggac cacgtcttcc
     3541 ttgtccgatc ctgcgaggaa gtacgagcgc gccttcgagc gcgcgaagtt ccggtagcgg
     3601 gagagaagcg tctcgaggca acgttcgtcg ccgtcccgga cgcgcttgac caggtcgagg
     3661 tcttcgagac cgtcgaggtc ttgcctttct tcggtccaaa cgctcatgac cattccccct
     3721 agtcgccgct ccaggtgctg cgtggcttcc tgcccggatg cctatgaaga ggctatttgt
     3781 ccggtttcga ccgaacaatg gcccgtccag gtggttgttg gcggctctaa ttgactaggt
     3841 acgcaagaac cttgcttagg tcaccggggg cgacgcctct tattttccca aatcggcggc
     3901 cttacatgca tgcttccggg acgacgaagg gggccggcga accggccccc ttcgtcgtga
     3961 gtcggccttc ggtcagcgct cgcagccctg ttcgtcgtcg cggcccggcc cgccgttgca
     4021 ccggtcgaag ccccgcccgc cgtccaggcg gtcgttgccg cggtcgccgc tcacgcggtc
     4081 gttgccgcca ccgcccgaga cccggtcgtt cccggccccg ccggagacgc ggtccttgcc
     4141 gccgttgccg agcactacgt cggggccgcc ggagccgcgc acggcgtcgt tcccgcggct
     4201 gcctccgacc gtgtcccggc cggccccacc gacgactatg tcggccccgc cgccaccccg
     4261 gaggcggtcg cggcccccgt tgccggacat ccggtcgttg ccgccctggc cgaggatcac
     4321 gtcgttgcct ccccggccgt tgatcgtgtc gttgccgccg aggccgcaga tcacgtcgtc
     4381 accgttcgtg cccgtgaggc gctctccggc ctcggtgccc gtgatcgtgc agggcccggt
     4441 cgtccagtgc ttgagggcgg tgtcggtgca gtcaccgccc tgggcgcacg gcgaggtcga
     4501 caccgacacg agctggtcgc ctgcctcctc ggacgagatc acggccatgg cgacgccgtt
     4561 ggcgtccgtc gtcgcgtcgc tctcctcgat ctcgccgacg ccggtcgacg tccacgtcac
     4621 cggagcgccc tccatcgggc tgccgttcgc gtccgtcacc gtcgcggtga gcgtgtggtc
     4681 gtcgccgacc tcgttcgtcg cctcggcggg tgtcaccgtc aggttcgccg cctgcgcgag
     4741 cggtgcgatc cacgtcttcg tgaccgtcgc ggagcactgt ccggccggcg agcccgcggg
     4801 atcacccgcg gcgcggtcgc actcgtcgac gtcgttcggc tcgttgccct gcaggtcgtc
     4861 gccgagggtc gcggtgatcg attgcgtccc ctcctccgtc gacgtcgtcg tcgcctggac
     4921 ctggccggcg ttgttcgtcg tctgctgcga ctgcgagctg agcgtgccgg ttcccgtgga
     4981 ggagagcgtg accgctcttc ctgcgagcgg ctggccgctg cggtcccgca cggtgcacgt
     5041 gaccgtgtgc tgcgtcccgg ccgggttcgt cgccgtggcc ggcgtgcaag agatcgtccg
     5101 cggctgcgtc acccacgtct tctcgaccac gtcggtcgcg tccgggtcgg tcgtcccgcc
     5161 ggcaccggcc ccgctctcgt cgcggctctc ggtcgcgtcc gcctccgcgg tggcgttcga
     5221 ggagtcggcg tcgatccacg agcgatacgt cgacgtcccg gtggcgtccg aggaaccgtt
     5281 gttcccgccg ccgccgtgcg tgaacgtgca gcttcctgcc gcagacgtcg tgcacgagaa
     5341 gtcggggttc gtgaggtcac cgctcgaatc gcggtcgttc gcgcccgtca cctcggcgtc
     5401 gacctccggt cccggcacga cgttgccgct cgagtcgcgg gccgtgcacg tgatcgtgtg
     5461 cgaggtaccg gtcgggttct gggccgtctc cggcgtgcag tcgagtgcgc ccgcggtcca
     5521 gaccttctgg acgacgtcgg tgtggtcggg ctcgttcgtc gagcccgcgc cgttgccgcc
     5581 ctcgtcgagg ttctcgccga cgtccgcttc cggggtcgcg ttgttgccgt ccaagtcgac
     5641 ccaggcgcgg tacgtcgtgg tgcccgcgcc cgcggtcgag cctccgccgg tcccaccgtg
     5701 cgtgaacgaa cagccgccgt tcccgtcggt ggtgcacgtg aagtccggag tctgcggcgt
     5761 gttgccgccg tcggggtcat tggcccccgt cgcctcgacg tcgacgttcg cgcccgaggc
     5821 ggtctggttg ttgccgtccc tgaccgtgca cgcgacctgg tgcgacgtgc ccgtcgggtt
     5881 catgtcggtc tcgggggtgc agtcgagacg tcccggcccc cag
//
LOCUS       DASQDO010000535         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_301193, whole genome shotgun sequence.
ACCESSION   DASQDO010000535 DASQDO010000000
VERSION     DASQDO010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301193"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VEV43_14710"
     CDS             complement(<1..477)
                     /locus_tag="VEV43_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYP24817.1"
                     /translation="MAKAVGIDLGTTNSVVAVLEGGEPTVIPNAEGARTTPSVVAFSK
                     GGEILVGEVAKRQAITNPDRTIRSIKREMGTDHKVDVDGKAWSPQEISARILMKLKAD
                     AEQYLGDKVTEAVITVPAYFGDAQRQATKEAGQIAGLEVLRVVAEPTAASLAYGLDK"
     gene            606..893
                     /locus_tag="VEV43_14715"
     CDS             606..893
                     /locus_tag="VEV43_14715"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HYP24818.1"
                     /translation="MADDFVEVYRGGGGQFRAEMLRSLLSGSGIESVITGSVASGLAS
                     NIGAIGEFAIVVRRAEADRAAELLRTFEEEGAGEIEELEWADDDEDEGAEE"
     gene            954..2267
                     /locus_tag="VEV43_14720"
     CDS             954..2267
                     /locus_tag="VEV43_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24819.1"
                     /translation="MTKKTIAALGVVACAALGPAAGAQTQDADTTQITAGAVGWSYGC
                     DVARHGRAVAFTSRATNLVAGDTNGKSDVFLWTVDGTQRVSVTSDEKQSSTGSFEASI
                     SDDGTRVAFTANRYRMQEPHFRPQGIFVRDAAAGLTTRVNVAVGGRRPNHRLSGRPAI
                     SGNGKHVAFYAAASNMVAGTNDTGWINAFVRSLDRGVTESVSLARDGDETRGTAYPDD
                     LSPDGRFAVFMADGRDIVRGDHNRAFDVFRHDRKTDRTIRVSVTSDGEEGGEKKFTGS
                     PAASANGRYVSFESRASLDPAYPTDERAIYLHDVVAGTTKLVSRGSEGPGNGPSDEPR
                     ISDDGRYVAFVSIADNLVPDDTNANYYPRAFGIDGFVRDVVAGKTFLVTVGDAGQQAD
                     GDTNDFDLSPDGDYVCWSSDATNLTVDANPSSDVFLRGQLNLGEE"
BASE COUNT          416 a          821 c          886 g          373 t
ORIGIN      
        1 cttgtcgagg ccgtacgcga ggctggcggc cgtcggctcg gcgacgacgc gcagcacctc
       61 gaggcctgcg atctggccgg cctccttcgt cgcctgtcgc tgggcgtcac cgaagtacgc
      121 ggggaccgtg atgaccgcct cggtcacctt gtcgccgagg tactgctcgg cgtcggcctt
      181 cagcttcatg aggatgcggg ccgagatctc ctgggggctc cacgccttgc catcgacgtc
      241 gaccttgtgg tcggtcccca tctcgcgctt gatcgaccgg atcgtcctgt cggggttcgt
      301 gatcgcctgg cgcttggcga cctcgccgac gaggatctcg ccgcccttcg agaaggcgac
      361 gacggacggg gtcgtccggg ccccctcggc gttcgggatg acggtgggct caccgccctc
      421 gaggacggcg accacggagt tcgtggtgcc gaggtcgatt cctactgcct ttgccatgtc
      481 gagctccttg tcgtggttcg ctttggaggt gggagttgag tcctcccaat gatgccaact
      541 tgagtgtcct actgtcaact tcggaacggg tccgggcatt cccgatccgt gccacaatcg
      601 gcgccatggc ggacgacttc gtagaggtct acagaggcgg cggagggcag ttccgggcgg
      661 agatgctccg ctcgctgctg tccgggagcg ggatcgagtc ggtcatcacg ggcagcgtcg
      721 cgtccggcct ggccagcaac atcggggcga tcggcgagtt cgcgatcgtc gtgcgacgcg
      781 cagaggccga ccgggcagcc gagctcctcc ggaccttcga ggaggaaggc gccggtgaga
      841 tcgaagagct cgagtgggcg gacgacgacg aggacgaggg cgccgaggaa taggcctcgc
      901 cggtgtaaag agttgagcgg aacctcccgg gcaggcgtgt cacgctgcta ggcatgacga
      961 agaagacgat cgcggccctc ggagtggtcg cgtgcgcggc cctcggcccc gccgcggggg
     1021 cccagacgca ggacgccgac acgacccaga tcaccgccgg cgccgtcggc tggagttacg
     1081 gatgtgacgt cgcccggcac ggccgtgcgg tcgccttcac gtcgcgcgcg acgaacctcg
     1141 tggccggcga cacgaacggg aagtccgacg tgttcctgtg gaccgtcgac ggcacccagc
     1201 gggtgagcgt gacgtcggac gagaagcaga gcagcaccgg cagcttcgag gcatcgatct
     1261 cagacgacgg gacccgggtc gcgttcacgg cgaaccggta ccggatgcag gagccgcact
     1321 tccgcccgca gggcatcttc gtgcgggacg cggcggcggg cctcacgacg cgggtgaacg
     1381 tcgccgtcgg cggccgccgg cccaaccatc gtctgagcgg aaggccggcg atctcgggca
     1441 acggcaagca cgtcgccttc tacgcggcgg cgtcgaacat ggtcgccggc acgaacgaca
     1501 ccgggtggat aaacgccttc gtacggtcgc tggaccgggg cgtgaccgag tccgtcagcc
     1561 tggcgcgcga cggcgacgag acccgcggca cggcctaccc cgacgacctc tcaccggacg
     1621 gcaggttcgc cgtcttcatg gcggacggac gggacatcgt ccggggggac cacaaccggg
     1681 ccttcgacgt cttccgccac gaccggaaga cggacaggac gatccgggtg agcgtcacct
     1741 ccgacggcga ggagggcggc gagaagaagt tcactggctc gccagctgcc tctgcgaacg
     1801 gtcgttacgt ctccttcgaa agcagggcgt cgctcgaccc cgcctacccg acggacgagc
     1861 gcgccatcta cctgcacgac gtggtcgccg ggacgaccaa gctcgtcagc aggggttcgg
     1921 aggggcccgg caacgggccc agcgacgagc cgaggatctc cgacgacggc cgctacgtcg
     1981 cgttcgtgtc gatagccgac aacctcgttc ccgacgacac caacgccaat tactaccccc
     2041 gcgcgttcgg gatcgacggc ttcgtccgcg acgtcgtcgc cggaaagacg ttcctggtga
     2101 ccgtcggcga cgccggccag caggcggacg gcgacacgaa cgacttcgac ctcagcccgg
     2161 acggggacta cgtttgctgg agcagcgacg cgacgaacct gaccgtcgac gcgaacccca
     2221 gcagcgacgt cttcttgagg gggcagctga acctcgggga ggagtagggc ctcggcttga
     2281 cgaagtcgtc cgggcgaagg taacaatccg ggcaaaaccg tcaccgatcc taggaggggc
     2341 cgtggagcgc aagcccgccg tgcgcgagct gttcatcgaa gagctggccg aggtgacggg
     2401 gggtgtcgcc gaccccctcc ggaaggtcaa ggacgctctc gtggaggtcc gcgactacct
     2461 catgaccacg tacggatgcg gcgaggagct catcga
//
LOCUS       DASQDO010000536         7106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_423702, whole genome shotgun sequence.
ACCESSION   DASQDO010000536 DASQDO010000000
VERSION     DASQDO010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423702"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VEV43_14725"
     CDS             complement(<1..308)
                     /locus_tag="VEV43_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24820.1"
                     /translation="MEKPTSGGAGSESRPWTVKLYVATLVASSLLLLLPTVSARTEWW
                     SYAPHVLLLLGVWVGSRIAWALTLAVDAFSFVMGLAIATAEGLILVGLLAVWIGLLLA
                     S"
     gene            complement(332..1255)
                     /locus_tag="VEV43_14730"
     CDS             complement(332..1255)
                     /locus_tag="VEV43_14730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYP24821.1"
                     /translation="MTTSPYGPMTLICNARSGRGGVGKALPEVRKYLEERELEHEVKF
                     TEGRGHATEIARSELEAGRKFLVAVGGDGTVHEVVNGMIRDDKAIVPDAVLGVVAAGT
                     GSDFIKTFGLPAMPAHAVAHLDGPESFPIDIGKITYVQDGAEVVRYFPNIAEVGLGAA
                     VVARAERLPRWLGPTVYFFAFWMQIRKHKQTQVVVDLVDRKYEGPMNNLVVANCQFFG
                     GGMKIAPKAVPTDGLLDIQIDKVRKREAIAIVPKIYKGEHLPHPDIKEAKRVRCSITS
                     ERPLPIEADGEVLGTTPATFEVLRDVIRLKV"
     gene            complement(1252..2631)
                     /locus_tag="VEV43_14735"
     CDS             complement(1252..2631)
                     /locus_tag="VEV43_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYP24822.1"
                     /translation="MSVASDLAALLDPSIVSTDEADLDAHASDWAPAGMLDRRRGKVP
                     PSPACVVRPRSTEEVASLLEWAHETRTPVVPWGGGSGVLASAVPGGRVVVDLSGLAHV
                     GDVDEKSRLVTAGAGVTGPALGEHLSDAGYHLGHEPQSLAISTVGGWIATRACGQLSA
                     RYGGIESLLAGFTAVLPGGRVVRSKTVPRRAAGPDVASLMLGSEGTLGIVTEADLRVS
                     PIPAERHDLCLRFEHMADGVAACRKLAQSELRPTLVRLYDAEDAAIFLRHFPDQSPGP
                     LLLVSFDGPRADVAAELCGGKRGDDHLVEHWWEHRNDAVGEFRKLMAGEGILGPHALV
                     DTVEVAGTWTVLRELYHSMKEKLSSLADIAGCHLSHVYPDGACLYFTLASACADDAEA
                     ESKLGTWWEAAMRTCLDAGGSISHHHGIGRVKARWLEEELGGWMDVLRAVKSSVDPHG
                     VMNPGALGL"
     gene            complement(2628..3824)
                     /locus_tag="VEV43_14740"
     CDS             complement(2628..3824)
                     /locus_tag="VEV43_14740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYP24823.1"
                     /translation="MTDAFIVDAVRTPMGKFGGALSSVRPDDLAAHVIAALVQRTGVP
                     GDAIDDVVWGAANQAGEDNRNVARMAALLAGLPVEVPGATVNRLCGSGLEAVNSAYRQ
                     IRADEGSVLIAGGSESMSRAPLVLAKAQTPFDRSQQMYDTTIGWRFVNPRLHEAWGSH
                     AMGETAEIVGHSYGISREDQDRFALTSHERAVAARDEGRFKDEIVPVEVPRRKGDPIV
                     VDQDEPPRPDTSIDRLAKLRPAFREGGTVTAGNSSGVNDGASAILLASGEAVDRHGWK
                     PMARIVTSAVAGVEPRMMGIGPIYATRKALERAGLSVDDVDLFELNEAFAAQSLACIR
                     GLELDPEKVNVNGGAIALGHPLGCSGARILTSLVHEMGRREARYGVATMCIGVGQGIA
                     TVVERA"
     gene            complement(3903..4115)
                     /locus_tag="VEV43_14745"
     CDS             complement(3903..4115)
                     /locus_tag="VEV43_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24824.1"
                     /translation="MRRLGKALIVMASAAAVALPATPALADDNPCKGQAPGPHCRPAC
                     FYYVYYDLGDPQNGVAPTVEIRETFC"
     gene            complement(4193..6877)
                     /locus_tag="VEV43_14750"
     CDS             complement(4193..6877)
                     /locus_tag="VEV43_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYP24825.1"
                     /translation="MAAARATATSSGFLPPDPAGAFEETYGFPFDEFQRRACNALVEG
                     SSVLVAAPTGAGKTVVGEFAAWLALQGTGKTFYTTPIKALSNQKYGDFVSLHGPSNVG
                     LLTGDNSLNGDAPVVVMTTEVLRNMIYEDSPELHALEYVVLDEVHYLQDRYRGGVWEE
                     ILIHLPVDVKIVSLSATVSNAEEFAEWLQTLRGRTEVIIEDKRPVEIKHWYFASDELL
                     PMFVRGKDGQPYPNPRGQEMDRRRRRGGQSRPGPKGRREREKRARIPWRSEVVERLHG
                     EAMLPAIYFIFSRKGCDEAVRQCMREGIRLTTADERKQIIDYADSRVADLNLNELDVL
                     GYGEWISGLANGLAAHHAGMIPPFKETVEELFARGLVKVVFATETLALGINMPARTVV
                     IESLMKFTGEKHELITPGQYTQLSGRAGRRGIDEIGHSVVLLQRFTPFDTITRLASTR
                     TYPLQSSFQPSYNMAVNLVRNYDRHEAEHLVNSSFAQFQTDRDVVRLERKRERSEAYL
                     ATYRERMECERGDFAEYMELRNRLAALERRGGRSRVHERVLEAIGELRAGDVIDLPAG
                     RRRGRYAVIEVSTRRSESVPRVLVLSEERTMIRLAPVDFREPPHAIARLRLPRDFNVR
                     DLSARRKLARQLAALEEAEPPPEPDDESRDEVKALQRAVARHPVAACPEVGRHIHFAE
                     RAARLEKEISGVDRQVARRTATLARRFDRVVDVLEDLGYVADWALTDKGERLARVYNE
                     SDLLVIEMLDRSMLAGLEPPELAAVCSSLVYETRGPETEAAVEMPSPRSREVHGELMR
                     LWRRIRRKEDERALELTREPDPGFAEKAFRWAAGWPLENVLSEDDPAGDFVRAVKQLI
                     DLLRQVEEVTADEALREVVGRAVDGLHRGVVAYSSLEV"
     gene            complement(6849..>7106)
                     /locus_tag="VEV43_14755"
     CDS             complement(6849..>7106)
                     /locus_tag="VEV43_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24826.1"
                     /translation="AQAHEEHEQGRGLFELGVEALDGELLEVGGDNSNLVLPQSTPPV
                     PEPGESPGSSTQTIEIPAGGGGGGQGGGGQGGGGGGQGDGN"
BASE COUNT         1023 a         2577 c         2412 g         1094 t
ORIGIN      
        1 gaagccagga gcaggccgat ccacacggcg agcaatccga cgaggatcaa gccctcagcg
       61 gtggcgatgg caagtcccat cacgaacgag aacgcgtcga ccgcgagcgt cagggcccag
      121 gcgatgcgac tcccgaccca gacccccagc agtagcagga cgtggggggc atacgaccac
      181 cattcggtgc gtgcgctgac cgtcggcagg aggagcagca gtgacgatgc cacgagggtg
      241 gctacgtaca gcttgacggt ccagggccgc gactcggacc ccgccccgcc gctcgtcggc
      301 ttttccacgg gttaagtcta gcgacaaccc gtcagacctt caggcggatc acgtctcgga
      361 ggacctcgaa ggtcgccggg gtcgtgccca ggacctcgcc gtcggcttct atcggcagcg
      421 ggcgctccga cgttatcgag cagcgcaccc gcttcgcctc cttgatgtcc gggtgcggca
      481 ggtgctcgcc cttgtagatc ttcggcacta tcgctatcgc ctcacgcttg cggaccttgt
      541 cgatctggat gtccagcagc ccgtccgtcg gcacggcctt cggcgcgatc ttcatgcccc
      601 cgccgaagaa ctggcagttc gccaccacga ggttgttcat cggcccctcg tacttgcggt
      661 cgacgaggtc gacgaccacc tgcgtctgct tgtgcttgcg gatctgcatc cagaaggcga
      721 agaagtacac cgtggggccg agccagcgcg ggagcctctc cgccctcgcc acgaccgcgg
      781 cccccagccc gacctccgcg atgttcggga agtaccgaac gacctcggcc ccgtcctgga
      841 cgtacgtgat cttgccgatg tctatcggga acgactccgg gccgtcgagg tgcgcgaccg
      901 catgcgccgg catcgcggga aggccgaacg tcttgatgaa gtccgacccc gtccccgcgg
      961 cgacgacgcc caggaccgcg tccggcacga tcgccttgtc gtcgcggatc atcccgttga
     1021 cgacctcgtg gacggtgccg tcgccgccga cggccacgag gaacttccgc ccggcttcga
     1081 gctcggagcg ggcgatctcg gtcgcgtggc cgcgtccttc ggtgaacttg acctcgtgct
     1141 cgagctcgcg ctcctcgagg tacttccgca cctccggcag agccttgccc acgcctcccc
     1201 tgcccgagcg ggcgttgcag atcagcgtca tcggcccgta cggcgacgtc gtcacagccc
     1261 cagcgccccc gggttcatca cgccgtgcgg atcgaccgag ctcttcaccg cgcgcaggac
     1321 gtccatccat ccgccgagct cctcttcgag ccagcgcgcc ttcacgcgac cgattccgtg
     1381 gtggtggctg atcgacccgc cggcgtcgag acacgtacgc atcgcggcct cccaccacgt
     1441 cccgagcttg gactccgctt ccgcgtcgtc cgcgcacgcc gacgcgagcg tgaagtagag
     1501 acaggccccg tcgggataga cgtgcgagag gtggcacccg gctatgtcgg cgagcgacga
     1561 cagcttctcc ttcatcgagt ggtagagctc gcgcaggacg gtccaggtcc cggcgacctc
     1621 gaccgtgtcg acgagagcgt gggggccgag aatcccctcc cccgccatca gcttgcggaa
     1681 ctcgccgacc gcgtcgttgc ggtgctccca ccagtgctcg accagatggt cgtcgccccg
     1741 cttccctccg cacagctccg ccgcgacgtc ggcgcgcggc ccgtcgaacg acaccaaaag
     1801 cagcggaccg ggcgactggt ccgggaaatg acgaaggaag atcgcggcgt cctccgcgtc
     1861 gtagaggcgc accaaggtcg gtcgcagctc ggactgcgcc agcttccggc acgcggctac
     1921 cccgtcggcc atgtgctcga agcgcaggca gaggtcgtgc cgctccgcgg ggatcgggct
     1981 caccctgagg tcggcctcgg tcacgatccc cagagtcccc tccgacccga gcatcaacga
     2041 cgcgacgtcg gggccggccg cccgccgcgg cacagtcttc gagcgcacga cccggccccc
     2101 ggggagcacc gcggtgaacc cggcgagcaa ggactcgatc ccgccgtagc gcgcggagag
     2161 ctgtccgcac gcccgcgtcg cgatccatcc gccgacggtc gagatcgcga gcgactgtgg
     2221 ttcgtggccg aggtggtatc cggcgtcgct caggtgttcg ccgagggccg ggccggtcac
     2281 gcccgccccc gcggtgacga ggcggctctt ctcgtcgacg tcgccgacgt gagcgaggcc
     2341 cgagaggtcg acgaccacgc gcccgccggg caccgcggac gcgagcaccc cggacccccc
     2401 gccccacggg accaccggcg tgcgcgtctc gtgcgcccac tcgagcagcg acgcgacctc
     2461 ttcggtgctc cgcggcctga ccacgcatgc cggcgacggc gggaccttgc cgcgccggcg
     2521 gtcgagcatc cccgcggggg cccagtcgga cgcgtgcgcg tcgaggtcgg cctcgtcggt
     2581 gctgacaatg ctgggatcga ggagcgcggc gaggtcggag gcgacgctca cgctcgttcg
     2641 acgaccgtag cgattccctg gccgacgccg atgcacatcg tcgctacgcc gtagcgagcc
     2701 tcgcgccggc ccatctcgtg cacgagggac gtgaggatgc gcgcgcccga gcagcccagc
     2761 ggatgcccca gcgcgatcgc gcccccgttc acgttgacct tctcggggtc gagctcgagc
     2821 ccccggatgc acgcaagcga ttgcgccgcg aaagcctcgt tgagctcgaa caggtcgacg
     2881 tcgtcgacgc tgagcccggc gcgctcgagg gccttgcgcg tcgcgtagat cggcccgatg
     2941 cccatcatcc gcggctcgac ccccgcgacc gccgacgtca cgatgcgcgc catgggcttc
     3001 cagccgtggc gatcgacggc ctcacccgac gcgagcagga tcgccgacgc cccgtcgttc
     3061 actcccgaag agttgcctgc cgtgacggtg ccgccctcgc ggaaggcggg gcgcagcttc
     3121 gcgagacgat cgatcgaggt gtcgggacgc ggcggctcgt cctggtcgac cacgatcggg
     3181 tcacctttgc gccgcgggac ctcgaccggc acgatctcgt ccttgaaccg tccttcgtcg
     3241 cgcgcggcga cggcgcgctc gtggctcgtc agcgcgaagc ggtcctggtc ctcgcgtgag
     3301 atgccgtagc tatggccgac gatctcggcc gtctcgccca tggcgtggct cccccacgcc
     3361 tcgtgcagcc gcgggtttac gaacctccag ccgatcgtcg tgtcgtacat ctgctgcgaa
     3421 cggtcgaacg gcgtctgggc cttggcgagc acgaggggtg cgcggctcat cgactcggat
     3481 ccgccggcta tcaggacgga gccctcgtcg gcgcggatct gccggtaggc ggagttgacg
     3541 gcctcgagcc cggagccgca gagacggttg acggtcgccc cggggacctc cacgggcaac
     3601 cctgccagca gcgccgccat gcgcgcgacg ttgcggttgt cctcgcccgc ctggttcgcg
     3661 gccccccaga ccacgtcgtc gatcgcgtcg ccggggacgc ccgtcctctg cacgagggca
     3721 gcgatcacgt gcgccgccag atcgtccggc cggacgctcg agagcgcgcc gccgaacttg
     3781 cccatcggcg tacgcaccgc gtcgacgatg aatgcgtcgg tcatgcggtc aatcctcgca
     3841 cggggccgga cgcgacgagg ccccgcgtgc tccgtgcggg gcctcgttcg agtggtaccg
     3901 gtctagcaga acgtctcgcg gatctcgacc gtgggggcta cgccgttctg cgggtcgccg
     3961 aggtcgtagt acacgtagta gaagcacgcc ggccggcagt gcgggccggg cgcctggccc
     4021 ttgcaggggt tgtcgtctgc gagcgcgggt gttgcgggca gcgcgacggc cgccgccgac
     4081 gccatcacga tcagggcctt gccgagtctc ctcatgcagt cctcctctta accgggccga
     4141 tccaacgcgg gcaggattct agtgacaaac cggacattcc tgccggttcg cgtcagacct
     4201 ccagcgacga gtaggcgacg acgccccggt ggaggccgtc gacggcccgg cccacgacct
     4261 cgcgcagggc ctcgtcggcc gtcacctcct caacctgacg caggaggtcg atgagctgct
     4321 tgaccgcgcg gacgaagtcg ccggcggggt cgtcctcgga cagcacgttc tcgagcggcc
     4381 agcccgccgc ccaccggaac gccttctcgg cgaagccggg gtcgggctcg cgcgtgagct
     4441 ccagcgccct ctcgtcctcc ttgcgacgga tccgccgcca cagccgcatc agctcgccgt
     4501 ggacctcgcg gctgcgcggc gacggcatct cgaccgcggc ctcggtctcg ggcccgcggg
     4561 tctcgtagac gagcgacgag cacaccgcgg cgagctccgg cggctccaac ccagcgagca
     4621 tggagcggtc gagcatctcg atcacgagca gatcggactc gttgtagacg cgggcgaggc
     4681 gctctccttt gtcggtgagg gcccagtcgg cgacgtagcc gaggtcctcg agcacgtcga
     4741 cgacgcggtc gaaccggcgt gccagcgtcg ccgtccgccg cgcgacctga cggtcgacgc
     4801 cggagatctc cttctcgagc cgcgccgctc gttccgcgaa gtggatgtgc ctgccgacct
     4861 cgggacaggc cgcgaccgga tgccgggcga ccgcgcgctg cagggctttc acctcgtcgc
     4921 gcgactcgtc gtccggctcg gggggcggct cggcctcttc gagcgcggcg agctgacgag
     4981 cgagcttgcg gcgcgccgag aggtcgcgga cgttgaagtc gcgcggcaga cgcagccgcg
     5041 cgatcgcgtg cgggggctct cggaagtcca ccggcgcgag ccggatcatc gtccgctcct
     5101 cggatagcac gaggacgcgc gggacgctct ccgatcgtcg cgtcgacacc tcgatcaccg
     5161 cgtagcgtcc gcggcgtcgc cccgccggga ggtcgatcac gtcgccggca cgcagctcgc
     5221 cgatcgcctc gagcacgcgc tcgtggacgc gggagcggcc cccgcgccgc tccagtgcgg
     5281 ccagacggtt gcgcagctcc atgtactcgg cgaagtcccc gcgctcgcac tccatccgct
     5341 cgcggtacgt agcgaggtac gcctcggagc gttcgcgctt gcgctccagt cgcacgacgt
     5401 cgcggtcggt ctggaactgc gcgaacgacg agttcacgag atgctcggcc tcgtggcggt
     5461 cgtagttccg cacgaggttc acggccatgt tgtaggaggg ctggaaagac gactggagcg
     5521 ggtaggtccg cgtcgacgcg agcctcgtga tcgtgtcgaa cggcgtgaag cgctggagca
     5581 gcacgaccga gtgcccaatc tcgtcgatgc ccctgcgccc ggcccggccc gacagctgcg
     5641 tgtactggcc cggcgtgatc agctcgtgct tctcgccggt gaacttcatc aggctctcga
     5701 tcacgaccgt gcgcgcaggc atgttgatcc cgagcgcgag cgtctccgtc gcgaacacca
     5761 ccttgacgag gccgcgcgcg aacagctcct cgacggtctc cttgaagggc gggatcatcc
     5821 cggcgtggtg cgcggcgagc ccgttcgcca ggccggagat ccactcgccg tagccgagga
     5881 cgtcgagctc gttgaggttc aagtcggcga cccgtgagtc cgcgtagtcg atgatctgct
     5941 tgcgctcgtc cgcggtcgtg agccggatgc cctcgcgcat gcactggcgc accgcctcgt
     6001 cgcagccctt gcgcgagaag atgaagtaga tcgccggcag catcgcctcg ccgtgcagcc
     6061 gctcgaccac ctccgagcgc cacgggatgc gcgcccgctt ctcgcgttcg cggcggccct
     6121 tcgggccggg cctcgactgc cctccccggc gtcgccggcg gtccatctcc tggccgcgcg
     6181 ggttcgggta cggctggccg tccttgccgc gcacgaacat aggcagcagc tcgtcgctcg
     6241 cgaagtacca gtgcttgatc tcgaccgggc gcttgtcctc gatgatgacc tcggtccggc
     6301 cgcgcagcgt ctggagccat tcggcgaact cctccgcgtt gctgaccgtg gccgagagcg
     6361 acacgatctt cacgtcgacg gggaggtgga tcaggatctc ctcccacacg ccaccgcggt
     6421 accggtcctg taggtagtgg acctcgtcga gcacgacgta ctcgagcgcg tgcagctcgg
     6481 gcgagtcctc gtagatcatg ttgcgcagga cctcggtcgt catcacgacc accggcgcgt
     6541 cgccgttcag agagttgtcg ccggtcagca ggccgacgtt cgagggcccg tgcagcgaga
     6601 cgaagtcgcc gtacttctga ttcgagagcg ccttgatcgg cgtcgtgtag aaggtcttgc
     6661 cggtcccctg cagcgcgagc cacgcggcga actcgccgac gacggtcttg cccgcgcccg
     6721 tgggggccgc gacgagcacg gacgatcctt cgacgagggc attgcaagca cgtcgctgga
     6781 actcgtcgaa cgggaacccg taggtctcct cgaaggcgcc ggcgggatcg gggggaagga
     6841 agccggagct agttgccgtc gccctggccg ccgccaccgc caccttggcc gccgccgccc
     6901 tggccgccgc caccaccgcc ggccgggatc tcgatcgtct gggtcgagct gccggggctc
     6961 tcgcccggct cgggcaccgg aggtgtgctt tgcggcagca cgaggttcga gttgtcgccg
     7021 ccgacctcta gcaattcgcc gtcgagggcc tcgaccccga gctcgaacag gccgcggccc
     7081 tgctcgtgct cctcgtgggc ctgggc
//
LOCUS       DASQDO010000537         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_117828, whole genome shotgun sequence.
ACCESSION   DASQDO010000537 DASQDO010000000
VERSION     DASQDO010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117828"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VEV43_14760"
     CDS             <1..326
                     /locus_tag="VEV43_14760"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYP24827.1"
                     /translation="RLAVDAGRDGGKHPAHSPELVRRPEALAGLGYPLLVAPSRQDVV
                     GETLGVPPVDRLEGTLALVALSVWAGASIVRVHDVKATVRTVRMVEAVMGTRAPAAPV
                     RGLWE"
     gene            396..767
                     /locus_tag="VEV43_14765"
     CDS             396..767
                     /locus_tag="VEV43_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP24828.1"
                     /translation="MTKRVLIVEDEEAVRELEKFILETQGYEVMEARDGLEGLAKAEF
                     RKPDLILLDLMMPDVSGGRMYDEMMQHPATSGIPIIVVTGKPDAHEIFDDQIGPDNVI
                     MKPFEADTLLGRIRQHIGDPE"
     gene            772..1545
                     /locus_tag="VEV43_14770"
     CDS             772..1545
                     /locus_tag="VEV43_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HYP24829.1"
                     /translation="MALFVAFTGVLGLIFGSFGTVAAWRIPRRETIVSGRSHCPKCGA
                     KITALENVPVLSWVMLRGKCRHCGNPISVRYPLIELTTGILFALSAAKFGLSVETFVY
                     AAFCWVLVVLTVIDLDHKLLPNRVVYPAFVVGWGGLVAAAIVDGSTDRLLDAAMGAAI
                     FGGTFFVIALVAPRGMGFGDVKLAFVLGTFLGYVRIGLVPLGMFLAFLTGTLGAIVVM
                     LVSGGDRKMQIPFGPFLALGTVLAIFAGAPLLDAYIDAL"
     gene            complement(1542..2681)
                     /locus_tag="VEV43_14775"
     CDS             complement(1542..2681)
                     /locus_tag="VEV43_14775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYP24830.1"
                     /translation="MTTPLAQTKDKPLVLVVDDDPDLRALAGIQLGDGGFDVIQAANG
                     RECLVLASTHSPDVILLDIMMPGMDGSEVLTALAGDPVTKDIPVVFVSALASPEDKVK
                     GLENGAVDYITKPLETREFVARVGVAARHRRRDRTDADGLGLAGREGFERKLREEVAR
                     ASRSNSALAVLLVDVDRLREFNELHGIVAGDVLLTEIAASLGSILRLSDSMYRYGEDE
                     FAIVLPDTDLPTAAVVAERCREVVSGVRSMRGRVTLSIGIAELQQGHAAEDLTARVQT
                     ALQRAKESGGDRIWRADDPRRHGLGPSSLARQLTEREWDILVHLSHRRTEQDIARRLG
                     IRPGTVRSHKARIRRKLHVAPDLRLTEYVRSHFKELESAPGQETG"
     gene            2832..3314
                     /locus_tag="VEV43_14780"
     CDS             2832..3314
                     /locus_tag="VEV43_14780"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HYP24831.1"
                     /translation="MENEELPEDDGAPINEEERELLQQDLTDVQVLKELLGPRGLKGA
                     VFFCPDCDEDHFLGWDLLAGNLKELLEEGESPVHEPAFNPDPHEYVSWDYARGFLDGY
                     ESFEEEEVGDLTNRIILELRDRGWRVDEVKGMLAAVGLQVPGEEPAEPGSDGAAPAPE
                     "
     gene            3317..>3475
                     /locus_tag="VEV43_14785"
     CDS             3317..>3475
                     /locus_tag="VEV43_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP24832.1"
                     /translation="MRVIAFANQKGGVAKTTTTLSVGVALAEQGLRVLAVDLDPQGAL
                     TYALGIDPD"
BASE COUNT          504 a         1199 c         1217 g          555 t
ORIGIN      
        1 gccggctcgc cgtggacgcc gggcgcgacg gcgggaagca cccggcccac tccccggagc
       61 tcgtgcgccg gccggaggcg ctcgcgggcc tcggctaccc gctgctcgtg gcgccgtcgc
      121 gccaggacgt ggtgggggag accctgggcg tgccgccggt cgaccggctc gaggggacgc
      181 tggcgctcgt cgcgctgtcg gtgtgggccg gcgcgtcgat cgtgcgcgtg cacgacgtga
      241 aggcgacggt gcggacggtg cgcatggtcg aggccgtgat ggggacgcgg gccccggcgg
      301 cccccgtgcg cggcctgtgg gagtaaccgg gcccgctctc tcccaggatg cccggtattc
      361 gctccggcgc gcccggcgct tgataccttt cccccatgac aaagcgcgtg ctgatcgtgg
      421 aggacgaaga ggcggtcagg gagctcgaga agttcatcct cgagactcag ggctacgagg
      481 tgatggaggc gcgggacggg ctcgagggcc tcgccaaggc cgagttccgc aagcccgacc
      541 tcatcctgct cgacctgatg atgcccgacg tctccggcgg acggatgtac gacgagatga
      601 tgcagcaccc cgcgacctcg ggcatcccga taatcgtcgt caccggcaag cccgacgcgc
      661 acgagatctt cgacgaccag atcgggccgg acaacgtgat catgaagccg ttcgaggccg
      721 acacgttgct gggccgcata cgccagcaca tcggcgatcc cgagtagcgc cttggcgctc
      781 ttcgtagcgt tcacaggcgt cctcggcctc atcttcggaa gcttcggcac cgtcgccgcg
      841 tggcgcatcc cgcggcgcga gacgatcgtc agcggccgca gccactgccc caagtgcggt
      901 gccaagatca ccgcgctcga gaacgtgccg gtgctgagct gggtcatgtt gcgcggcaag
      961 tgccgtcact gcggcaatcc gatctcggtg cgctacccgc tgatcgagct cacgaccggc
     1021 atcctgttcg cgctgagcgc ggcgaagttc gggctgtcgg tcgagacgtt cgtgtacgcg
     1081 gcgttctgct gggtcctcgt cgtgctcacg gtgatcgacc tcgaccacaa gctgctgccg
     1141 aaccgcgtcg tgtacccggc gttcgtcgtc ggctggggcg ggctcgtcgc ggctgcgatc
     1201 gtcgacggca gcaccgaccg tctcctcgac gccgcgatgg gggccgcgat cttcggcggc
     1261 acgttcttcg tcatcgcgct ggtcgcgccg cgcgggatgg gcttcggcga cgtcaagctc
     1321 gcgttcgtgc tggggacgtt cctcggctac gtccggatcg gcttggtccc gctcgggatg
     1381 ttcctcgcgt tcctgacggg cacgctcggc gcgatcgtcg tcatgctcgt cagcggcggc
     1441 gaccgcaaga tgcagatccc gttcggcccg ttcctcgcgc tggggacggt gctggcgata
     1501 ttcgccggag cgccgctgct cgacgcgtac atcgacgcgc tctagccggt ctcctgcccg
     1561 ggcgcgctct cgagctcctt gaagtgcgac ctcacgtact cggtcagcct gaggtcgggt
     1621 gcgacgtgga gcttgcgccg gatgcgcgcc ttgtggctcc ggaccgtgcc cggccggatc
     1681 ccgagacggc gggcgatgtc ctgttccgtg cgccggtgcg agaggtggac gaggatgtcc
     1741 cactccctct cggtgagctg gcgcgcgagc gacgacggcc cgaggccgtg ccgccgcggg
     1801 tcgtccgccc gccagatgcg gtcgccgccc gactccttcg cgcgctggag cgcggtctgg
     1861 acgcgggcgg tgaggtcctc ggcggcgtga ccctgctgga gctcggctat cccgatcgag
     1921 agcgtgaccc gtccgcgcat cgaacggacg cccgacacca cctcccggca gcgctccgcg
     1981 accacggccg cggtcggcag gtcggtgtcc ggcaacacga tcgcgaactc gtcctcgccg
     2041 tagcggtaca tcgaatcgga caggcgcagg atcgagccga ggctcgccgc aatctcggtt
     2101 aggaggacat cgccggcgac gatcccgtgc agctcgttga actcgcgcag gcgatcgacg
     2161 tcgacgagca gcacagcgag cgccgagttg ctgcgcgacg cgcgtgcgac ctcctcgcgg
     2221 agcttccgct cgaagccctc gcgtccggcc aggccgaggc cgtccgcgtc cgttcggtcg
     2281 cgccgccggt gccgggccgc gacgcccacg cgggcgacga actcgcgcgt ctcgagcggt
     2341 ttcgtgatgt agtcgacggc cccgttctcg aggcccttca ccttgtcctc cggggacgca
     2401 agggccgaga cgaagacgac cgggatgtcc ttggtcacgg ggtcaccggc cagcgccgtg
     2461 agcacctcgc ttccgtccat ccccggcatc atgatgtcga gcaggatcac gtcgggcgaa
     2521 tgcgtcgacg cgagtaccag gcactcgcgg ccattggcag cctggatgac gtcgaaaccc
     2581 ccgtcgccca gctggatccc ggcgagcgcg cggagatcgg gatcgtcgtc gacgaccagg
     2641 acgagcggct tgtccttcgt ttgggcgagc ggtgtcgtca tctgcgtagc gctcccggcg
     2701 ccgtttccac gtatccgaac caagctccca cggttgagcg tcccgtggaa tgaaccgaac
     2761 gggccatttc ggactaagca gatacaccta gacgcatttc gctcgtcact ccgccccctt
     2821 acgatgagca catggagaac gaggagctgc ccgaagacga cggcgcgccg ataaacgaag
     2881 aggagcgcga gctactccag caggacctca ccgacgtgca ggtcttgaag gagctgctcg
     2941 gcccccgcgg cctcaagggc gccgtcttct tctgcccgga ctgcgacgag gaccacttcc
     3001 tcggctggga cctcctcgcg gggaacctca aggagctcct cgaagagggc gagtcgccgg
     3061 tgcacgagcc cgcgttcaac cccgacccgc acgagtacgt ctcgtgggac tacgcccgcg
     3121 gcttcctcga cggctacgag tcgttcgagg aggaggaggt cggcgacctc acgaaccgga
     3181 tcatcctcga gctgcgcgac cgcggctggc gcgtcgacga ggtgaagggg atgctcgccg
     3241 ccgtcggcct gcaggtgccg ggcgaggagc cggcggagcc gggctccgac ggcgccgctc
     3301 ccgcgccgga gtagccgtgc gcgtcatcgc gttcgcgaac cagaagggcg gggtcgccaa
     3361 gacgaccaca accctttcgg tcggcgtagc gcttgccgag cagggtttga gggtgctcgc
     3421 ggtcgacctc gacccacaag gcgctctgac gtatgccctg gggatcgatc ccgac
//
LOCUS       DASQDO010000538         7161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_424162, whole genome shotgun sequence.
ACCESSION   DASQDO010000538 DASQDO010000000
VERSION     DASQDO010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424162"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VEV43_14790"
     CDS             complement(<1..1040)
                     /locus_tag="VEV43_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HYP24833.1"
                     /translation="MGAVGTLIVIAVLLLVAAFTAMAETAISRIGRVKALHLTQEKPG
                     RRSERLLKIVEDPAPYLNVVLFVTLLAHITGTTLAASLAIRELGEGGEPIAAGVMTFL
                     IFVLAEVVPKTFTVQRTETAGLIAARPVYFFGRLLMPVAKVLILVANALMVLLPGRGL
                     PKGPFVTEDEIRHMVDVAEEEEEIEEEERELIHSVFEFGDTVVREVMVPRPDMIVIRA
                     DAALDEALGTIIQGGYSRVPIYEGDNDNIIGVLYAKDLLKRIHENKREAKVAALGRAP
                     LFIPEQKKVAELLREMQEQHVHMAIVVDEYGGTAGLVTIEDLIEEIVGEIVDEYDVEA
                     PKVERLPDGGLRV"
     gene            complement(1040..1480)
                     /gene="ybeY"
                     /locus_tag="VEV43_14795"
     CDS             complement(1040..1480)
                     /gene="ybeY"
                     /locus_tag="VEV43_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HYP24834.1"
                     /translation="MDVFLANEQGIPLDEPLLAALARHVLESEDVDGSAELSLLFVGA
                     DHIRRLNARYAGDDYATDVLAFPMAEDEDDDDPLMVGDVVVCPEVARDNAAKAGSTLE
                     RELRMLVVHGTLHLLGYDHQNDAQRTAMDKRMNEIIASFEPVAG"
     gene            complement(1480..2454)
                     /locus_tag="VEV43_14800"
     CDS             complement(1480..2454)
                     /locus_tag="VEV43_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYP24835.1"
                     /translation="MLTTTQVKILVPGHHDMVRLVGARDELLKVIEAAFATRILVRGN
                     EIVISGEPAEAERVSVLFEELLDLLEKGQELTKDAVGRSIEMVKRDEGVKPSEVLSDT
                     VVVTQAGKPIRPKTEGQKRYVDSIRKNTIVFGIGPAGSGKTYLAVAAAIRSLQSGEVS
                     RIVLTRPAVEAGERLGFLPGDLAAKIDPYLKPLYDALYEMLDPEVFHRYAEQGTVEIA
                     PLAFMRGLTLNDSFIILDEAQNTTPEQMQMFLTRLGFGSKAVVTGDVTQVDLPSNTRS
                     GLIVIEEILKDIAGVGYIHLDARDVVRHKIVQDIVEAYRVYADASKRR"
     gene            complement(2570..2911)
                     /locus_tag="VEV43_14805"
     CDS             complement(2570..2911)
                     /locus_tag="VEV43_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HYP24836.1"
                     /translation="MSDCLFCKIAAGEIPSDTLFKSDEVVAFRDINPAAPTHVLVIPV
                     RHIASAADLTASDAPVLAEMFETMASLAESENLSGGWRVVTNVGGDAGQSVHHLHFHL
                     IGGRTMSWPPG"
     gene            complement(2908..3375)
                     /locus_tag="VEV43_14810"
     CDS             complement(2908..3375)
                     /locus_tag="VEV43_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24837.1"
                     /translation="MDHVERAVAALPDVALATDVIVGFPTETDEAFEATMDVVRRLRF
                     SKLHVFRYSPRPGTGAAALPDAVPPAVKKERSKRLIDLGNGIRGEFLRAHLGVPLEVL
                     VEDEREVDGVTVSSGQTSDYVRVWFEGGGMLGSLVQVRGTGIRSDGVRGELVS"
     gene            complement(3391..4179)
                     /locus_tag="VEV43_14815"
     CDS             complement(3391..4179)
                     /locus_tag="VEV43_14815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYP24838.1"
                     /translation="MRVAFSTLGCRLNQAETDSMTEQLEALGVERVADAADAGVVVVN
                     TCTVTREATKASRTAIRRAIAANPDSKVVVIGCYAVSDPDEVRAIEGVDLVLGNDAKD
                     SLPEHLGLSAMPAPLMQIGIRREAPPKIRANLKVQTGCDEWCTFCIIPTTRGPLKSTD
                     EDALLAEARARAAAGARELVLTGVHLGKYTFDHGGDERSLVRLFDRLLAIDGVARIRL
                     SSIFARHLTPEVLSFMASEPRMCRYLHVPLQSGDPGVLADMHRS"
     gene            complement(4192..4869)
                     /locus_tag="VEV43_14820"
     CDS             complement(4192..4869)
                     /locus_tag="VEV43_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HYP24839.1"
                     /translation="MPRDESHHALKVLRVSPPDVVTVTDGRGVVARCAAVRVEGDLLV
                     AEVLERDVHRRPRPELVVYQGAAKGAKADEVVEKLAELGVAEVRVYESKRSVARWDDA
                     KVARLNERWEAITRSAAKQSRNPFVTHAVAGLSWSQVVDEIGREPLPVVLWEEATLPL
                     RTALLDGVERIAFVVGPEGGLEPAEAEALARAGAQLVSLGPHILRTENAALVTAAAVL
                     SRYGAIG"
     gene            complement(4926..6017)
                     /gene="dnaJ"
                     /locus_tag="VEV43_14825"
     CDS             complement(4926..6017)
                     /gene="dnaJ"
                     /locus_tag="VEV43_14825"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HYP24840.1"
                     /translation="MADYYEVLGVPRDATPDDVKKAYRRLARQYHPDANPGDPDAAER
                     FKEINAANEVLSDPAKRQRYDRFGDENAGAGGFADFGNISDIFATFFGGGFGGGAARR
                     GPARGADVLAEIEITLEEAAEGVEREVEVTTLGTCGECGGDGAAPGTFPTRCSDCGGT
                     GELRSVRRTMLGNVMTAAPCARCGGTGQEVTTPCSECGGRGRVEVTDTLTVPIPPGVE
                     DGTRLRVTGRGEAGFRGGQSGDLYVGIDVLPHEVFRRAGADLACEVSVPMTVAALGGT
                     ITIPTLDGDEEVDVAPGTQPGEVKRLRGRGMPYLGGRGRGELVALLRVETPTDLDDEQ
                     VELLQRFAKLRDEHTGTKGFFDRIKEAFQ"
     gene            complement(6023..7072)
                     /gene="hrcA"
                     /locus_tag="VEV43_14830"
     CDS             complement(6023..7072)
                     /gene="hrcA"
                     /locus_tag="VEV43_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HYP24841.1"
                     /translation="MPPETERLTDRRALILRAIVSHYVGTGEPVGSKVLVERYRLGVS
                     PATVRNEMAALEDAGYIYQPHTSAGRIPTDAGYRYFVDEMPEGARLPAAEAHRIRQFF
                     VEPRWELEEALRQTAALLSSLTNHAAVVFAPALDRSLIRHVELVELPGGRAMLIAVTD
                     TGRVENHVVMLPEDVGEGDLLAASEGMATLLNGVALEDAAVTVRERSGDLPHHLGPML
                     RLAADAFDSPAEVKAHERFFLEGASNIVDETKFSDLESVRKVISALEHRRLLLEVLAD
                     AFSLGGVSVRIGAENPATEMRLCSIVTASYGTGESALGSLGIVGPTRMDYRRTIAAVH
                     EVSSHLGQMLAGLGI"
BASE COUNT         1053 a         2549 c         2426 g         1133 t
ORIGIN      
        1 acgcgcaggc cgccgtcggg gaggcgctcc accttcgggg cctcgacgtc gtactcgtcg
       61 acgatctccc cgacgatctc ctcgatgagg tcttcgatcg tcacgaggcc ggccgttccg
      121 ccgtactcgt cgacgacgat cgccatgtgg acgtgctgct cctgcatctc gcgcagcagc
      181 tcggcgacct tcttctgctc ggggatgaac agcggcgcgc ggccgagcgc ggcgaccttc
      241 gcctcacgct tgttctcgtg gatccgcttg aggaggtcct tcgcgtagag gacgccgatg
      301 atgttgtcgt tgtcgccttc gtagatgggg acgcgcgagt agccgccttg gatgatcgtc
      361 ccgagcgctt cgtccagcgc cgcgtcggca cggatcacga tcatgtcggg gcgcggcacc
      421 atcacctctc gcacgaccgt gtcgccgaac tcgaagaccg agtggatgag ctcgcgctcc
      481 tcctcctcga tctcctcttc ctcctccgcg acgtcgacca tgtgacggat ctcgtcctcg
      541 gtgacgaagg gtcccttagg gagaccgcgc ccgggcagca gcaccatcag ggcattcgcc
      601 acgaggatca ggaccttcgc caccggcatc agcagccgcc cgaagaagta gacgggccgg
      661 gccgcgatga gcccggcagt ctcggtccgc tgcacggtga aggtcttggg gaccacctcc
      721 gctagcacga agatcagaaa cgtcatgacg ccggccgcga tcggctctcc gccctctccg
      781 agctcgcgga tcgcgaggct cgcggccagc gtcgtgccgg tgatgtgcgc gaggagcgtg
      841 acgaacagca cgacgttcag gtagggggcc gggtcctcca cgatcttcag gaggcgctcg
      901 gagcggcggc cgggcttctc ctgcgtcagg tggagagcct tgacccgccc gatcctcgat
      961 atggcggtct cggccatcgc ggtgaacgcc gcgacgagca gcagcaccgc gatgacgatc
     1021 agggtcccga cggcccccat cagccggcca ccggctcgaa ggacgcgatg atctcgttca
     1081 tccgcttgtc catcgccgtg cgctgcgcgt cgttctggtg gtcgtagccg agcaggtgca
     1141 gcgtcccgtg gacgacgagc atccggagct cgcgctcgag cgtcgatccc gccttcgcag
     1201 cgttgtcgcg cgccacctcc gggcacacga cgacgtcccc gaccatcagc gggtcgtcgt
     1261 cgtcttcgtc ctcggccatc gggaacgcga ggacgtcggt cgcgtagtcg tcgcccgcgt
     1321 agcgcgcgtt gaggcggcgg atgtggtccg ctcctacgaa caggagagac agctcggccg
     1381 agccgtcgac gtcctccgat tcgagcacat gtcttgcgag cgccgccaga agcggctcgt
     1441 ctaacgggat gccttgctcg ttcgccaaga agacgtccat cagcggcgct tcgacgcgtc
     1501 cgcgtacacg cggtacgcct ctacgatgtc ctgcacgatc ttgtggcgga cgacgtcgcg
     1561 tgcgtcgaga tggatgtagc ccacgcccgc gatgtccttg aggatctcct cgatcacgat
     1621 caggcccgag cgtgtgttgg acgggaggtc gacctgggtc acgtcgccgg tgacgacggc
     1681 cttcgagccg aagccgagcc gcgtgaggaa catctgcatc tgctcgggcg tcgtgttctg
     1741 cgcctcgtcg aggatgatga acgagtcgtt gagggtcagg ccgcgcatga aagccagcgg
     1801 ggcgatctcg acggtcccct gctccgcgta gcggtggaag acctcggggt cgagcatctc
     1861 gtagagcgcg tcgtacagcg gcttgagata cggatcgatc ttcgccgcga ggtcgcccgg
     1921 gaggaagccg aggcgctcgc cggcctcgac cgcgggccgc gtcagtacga tgcgcgagac
     1981 ctcgccggac tggagcgacc ggatggctgc cgcgaccgcg aggtaggtct tgccgctgcc
     2041 ggcggggccg atgccgaaca cgatcgtgtt cttgcggatc gagtcgacgt agcgcttctg
     2101 gccctcggtc ttgggccgga tcggcttgcc cgcctgggtg acgacgacgg tgtcggagag
     2161 gacctccgac ggcttgacac cctcgtcgcg cttgaccatc tcgatagagc gtccgacggc
     2221 gtccttcgtc agctcctggc ccttctcgag caggtcgagc agctcctcga acaggacgga
     2281 gacgcgctcg gcctcggcgg gctcgcccga gatgacgatc tcgttgccgc gcacgaggat
     2341 gcgggtcgcg aaggcggcct cgatgacctt gaggagctcg tccctcgccc cgacgagccg
     2401 gaccatgtcg tgatggccgg ggacgaggat cttgacctgg gtagtcgtaa gcatgtctat
     2461 gaggaacaag attgtacttt gtcctgttcc tgagggggtt tggcctctct agagggcgtc
     2521 ggtggcgcgg aacgtgcgcc gtgcgtacgg aatccgccgg cgagcggggc taaccggggg
     2581 gccaggacat cgtgcggccc ccaatcaggt ggaagtgcag gtggtgcacg ctctggcccg
     2641 cgtcgccgcc tacgttcgtc actactcgcc agccgccgct caggttctcg ctctcggcca
     2701 ggctcgccat cgtctcgaac atctccgcaa gcaccggcgc atccgacgca gtcaggtcgg
     2761 ccgcggacgc gatgtgcctt accgggatca caaggacgtg cgtgggggcc gcgggattga
     2821 tgtcccggaa cgccaccact tcgtcggact tgaacaacgt gtccgacgga atctctccgg
     2881 ctgcgatctt gcagaacaga cagtcgctca cgagaccaat tcccctctca ctccgtcgga
     2941 gcggatgccg gtcccccgga cctgcaccag cgagcccagc atcccgccgc cttcgaacca
     3001 caccctcacg tagtcgctcg tctgccccga cgagaccgtc acaccgtcca cctcgcgctc
     3061 gtcctcgacc aggacctcca gtggcacgcc caagtgcgcg cgcaggaact ccccgcgtat
     3121 cccgttgccg aggtcgatca gacgcttcga gcgttccttc ttcaccgccg gcggcacggc
     3181 gtccggcagc gcggcggcac ccgtgccagg gcgcggcgag tacctgaaca cgtgcagctt
     3241 cgagaaccgc aaccgccgga cgacgtccat cgtcgcctcg aaggcctcgt cggtctcggt
     3301 cgggaagccg acgatcacgt cggtcgcgag cgcgacgtcc gggagagcgg cgacagcgcg
     3361 ctcgacgtgg tccacgtact cgccgatctc ttagctgcgg tgcatgtcgg cgagcacccc
     3421 tgggtcgccc gactgcaagg gtacgtggag gtagcggcac atccgcggct ccgatgccat
     3481 gaacgacagc acctccggcg tcaggtgccg ggcgaagatc gacgacagac ggatccgcgc
     3541 cacgccgtcg atcgccagca gccgatcgaa cagccggacg aggctgcgct cgtcgccacc
     3601 gtggtcgaac gtgtacttgc cgaggtggac gccggtgagc acgagctcgc gagcaccggc
     3661 cgcggcccgt gcgcgcgcct ccgccagcaa ggcgtcttcg tccgtgctct tcagcggccc
     3721 ccgcgtcgtc gggatgatgc agaacgtgca ccactcgtcg cacccggtct ggaccttgag
     3781 gttcgccctg atcttcggcg gcgcctcgcg ccgtatgccg atctgcatca acggtgccgg
     3841 catcgcgctc aggccgaggt gctcgggcag ggagtccttc gcgtcgttgc ccagcacgag
     3901 gtcgacgccc tcgatcgcgc ggacctcgtc ggggtcggac accgcgtagc agccgatcac
     3961 cacgaccttc gaatcggggt tcgcggcgat cgcccgccgg atcgccgtgc gggacgcctt
     4021 cgtggcctcg cgcgtcaccg tgcacgtgtt gacgacgacg actcccgcat ccgccgcgtc
     4081 ggcgacgcgt tcgacgccga gtgcttcgag ctgctcggtc atcgagtcgg tctcggcctg
     4141 gttcagcctg cagccgaggg tggagaacgc gactcgcatg ggacggcgaa actatccgat
     4201 ggcgccgtag cgagacagca ccgcggccgc ggtcacgagc gcggcgttct cggtcctgag
     4261 gatgtggggg ccgagcgaca cgagctgcgc tccggccctc gccagcgctt cggcctccgc
     4321 cggctcgagc ccgccttccg gcccgacgac gaacgcgatc cgctcgacgc cgtcgagcag
     4381 agccgtccgc agcggcagcg tcgcctcctc ccacaacacg accggcaggg gctcgcgccc
     4441 gatctcgtcg acgacctgtg accacgacag cccggcgacg gcatgcgtga cgaacgggtt
     4501 gcgcgactgc ttcgcggccg accgggtgat cgcctcccac cgttcgttca gccgcgccac
     4561 cttcgcgtcg tcccagcgcg cgaccgaacg cttcgactcg tagacgcgca cctcggcgac
     4621 accgagctcg gcgagcttct cgaccacctc gtctgccttg gcccccttgg cggcaccctg
     4681 gtagacgacg agctcgggcc gggggcgccg gtgcacgtcg cgctcgagca cctcggcgac
     4741 caggaggtcg ccctcgacgc gcacggccgc acagcgcgcg accacgccgc ggccgtccgt
     4801 aaccgtgacg acgtccgggg gcgagacgcg caagaccttc agcgcgtgat gagactcgtc
     4861 gcggggcaac gcgacctcgc cgtcccgcca cgcgtccggc ggagcgaaga accacgggtg
     4921 cgtgcttact ggaacgcttc cttgatcctg tcgaagaagc ccttggtgcc ggtgtgctcg
     4981 tcgcgcagct tcgcgaagcg ctggagcagc tcgacctgct cgtcgtcgag gtccgtcggc
     5041 gtctcgaccc tgagcagcgc cacgagctcg ccgcggccgc ggcccccgag atacggcatg
     5101 ccccggcccc gcaggcgctt gacctcaccc ggctgcgtgc ccggtgcgac gtcgacctcc
     5161 tcgtcgccgt ccagcgtggg gatcgtgatc gtccctccga gggccgcgac cgtcatcggg
     5221 acgctcacct cgcacgcgag gtcggcgccg gcgcgtctga agacctcgtg cgggagcacg
     5281 tcgatgccta cgtagaggtc gcccgactgc ccgccgcgga agcccgcctc gccgcggccg
     5341 gtcacacgca gacgcgtccc gtcctcgact cccggcggga tcggcaccgt gagggtgtcg
     5401 gtcacctcga cgcgcccgcg gcccccgcac tcggagcagg gtgtcgtgac ctcctgaccc
     5461 gtgccgccgc agcgcgcgca cggcgcggcc gtcatcacgt tgccgagcat cgtgcggcgg
     5521 accgacctga gctcgcccgt cccgccgcag tcggagcagc gcgtcggaaa cgtccccggc
     5581 gcggcgccgt cgccaccaca ctcgccacag gtcccgagcg tcgtcacctc gacctcgcgc
     5641 tcgaccccct ctgcggcctc ctccagggtg atctcgatct cggcgaggac gtcggcgcca
     5701 cgggccggcc cgcgccgcgc cgctcctccc ccgaagccgc cgccgaagaa cgtggcgaag
     5761 atgtcggaga tgttgccgaa gtcggcgaac ccaccggcgc cggcgttctc gtcgccgaac
     5821 cggtcgtagc gctgacgctt cgcggggtcg gacagcacct cgttcgcggc gttgatctcc
     5881 ttgaagcgct ccgcggcgtc gggatcgccg gggttcgcgt cggggtggta ctgccgcgcc
     5941 agcctccggt acgccttctt tacgtcgtcg ggggttgcgt cccgcgggac cccgagcacc
     6001 tcgtagtagt cggccatccc cgtcagatac ccaggccggc gagcatctgc ccgaggtgcg
     6061 acgagacctc gtgcacggcc gcgatcgtcc gccggtagtc catccgggtc gggccgacga
     6121 tgccgagcga gcccagcgcg ctctcgccgg ttccgtaaga ggccgtgacg atcgagcaca
     6181 ggcgcatctc ggtcgccggg ttctccgcgc cgatccggac cgagacgccg ccgagcgaga
     6241 acgcgtcggc gagcacctcg agcaggagcc tgcggtgctc gagcgcgctg atgaccttgc
     6301 gcacgctctc gaggtcggag aacttcgtct cgtcgacgat gttcgacgcc ccctccagga
     6361 agaaccgctc gtgcgccttg acctcggcag gcgagtcgaa ggcatcggcg gccagtcgca
     6421 gcatcgggcc gaggtgatgc ggcaggtcgc cggaacgttc ccggaccgtg acggccgcgt
     6481 cctcgagcgc cacgccgttc aggagcgtgg ccatcccctc ggacgccgcg agcaggtcgc
     6541 cttccccgac gtcctcgggc agcatcacga cgtggttctc gacgcggccc gtgtcggtga
     6601 ccgcgatcag catggcgcgg cccccgggca gctcgacgag ctcgacgtga cggatcaggc
     6661 tccggtcgag cgccggcgcg aacacgaccg ccgcatggtt cgtgagcgac gacaggagcg
     6721 cggccgtctg gcgcagggcc tcttcgagct cccagcgcgg ctcgacgaag aactgccgga
     6781 tgcggtgggc ctcggcggcc gggagccgcg cgccctccgg catctcgtcg acgaagtacc
     6841 ggtagccggc gtcggtcggg atccgcccgg ccgaggtgtg cggctggtag atgtagccgg
     6901 cgtcttcgag ggccgccatc tcgttgcgga cggtcgccgg cgagacgccg aggcggtacc
     6961 tctcgacgag caccttcgag ccgacgggct cgccggtccc gacgtagtgc gacacgatcg
     7021 cgcgcaggat cagcgcgcgg cggtcggtga ggcgctcggt ctcggggggc atgggcccga
     7081 tcctaccgga attagcactc tcagcggtgg agtgccagag agctagccga ccatcccggc
     7141 caggtggacg tcgtggtccc g
//
LOCUS       DASQDO010000539         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_484653, whole genome shotgun sequence.
ACCESSION   DASQDO010000539 DASQDO010000000
VERSION     DASQDO010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484653"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..712
                     /locus_tag="VEV43_14835"
     CDS             299..712
                     /locus_tag="VEV43_14835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYP24842.1"
                     /translation="MPRTRWDAVRDGDVRMPVNETLGFELDRSADGVAYDWTVPSEYC
                     HGAGNLQGGMLAALADAVLGGVASTVLPEDRYPVLADMNLSILRPAPAGARLRATGRV
                     VKAGRRVIFTEAEITDASTGKLIAKASGTALAAEA"
     gene            729..4187
                     /locus_tag="VEV43_14840"
     CDS             729..4187
                     /locus_tag="VEV43_14840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYP24843.1"
                     /translation="MSGFVHLHAHTVWSLRDGAIPAEVLPHFAKDQGYEAIAMTDHDS
                     LTGAVRFTKACRSAGIKPIYGVELTVRREPPEDARPERRLKNGVEERSGGRPEAAGTN
                     WNGLDHVTVIAKNATGYANLCRLISDAHLSHERDRPETTRTKIAERAEGLFVLSGCSR
                     GEVARKANAGDIPGAIEAAKRWRDEIGPGYRLEVFDHRGYGHRMLRDRLLAVARESNT
                     RPVATNDVHYATPRYAGVHELLHSIKEIVPLSRTHALRPNSEYYLKDPAEMRALFADA
                     PEAADETRRIADECDFELDLGTYHFPDFELPEGDLPEWVLERRCYEGVYRRFDRITNE
                     VEDRLQHELRMINKLGWPSYFLMVADIVDHVKTKLGIRCACRGSAAGSLVCYLLGISD
                     VDPVRYDLLFERFMNTRREELPDIDVDVESHRREEVLAHVLDTYGADQTAMCCMVDTF
                     RARMAIREVGKALGLPPEEIDLVAKAFPRVRASDIPQAIQRLPELANSNLDAGQLEQL
                     FRLCIEIDGFPRHLALHPSGVILSANDLADRVPMQESFGGFTMLQADKDDVEELGLVK
                     LDVLGVRMLSSIAHAVAEIKRARGETIDLDRLPHDDDKTFDLVRSSRTLGCFQIESPG
                     QRELLARFQPGRFEDLIVDISLFRPGPVKSDMVSPFLERRHGFEETRYVHPKLRPILA
                     ETNGVVVYHEQVIRVIAAATGCSLDDADYVRRHLDADQPGRVIWEGVAQPIEITPPDS
                     SVESWFKASAVQNGFTRGQADELWKEVFAFASFGFCKAHAAAFALPTYLSTWLKAHYP
                     AEFLAGILTHDPGMYPRRLIVADARHFGTPILPVDVNASHATYRVERAGDKVGIRLAL
                     SEVRDITEAEIDSLIEHRPYESLEDLWRKATVSRPVLENLVHLGALDSIKGARTRREL
                     LWRAVDLTTRTPAQKRTRRRKRVAVESPPQLAFDLEEPIAETLPGLPEYSELEETEAE
                     LEITGIDARRHMLDLYRPLLAEIGCVPADRLAAQKNDAQVWVAGVKVSSQTPAIRSGQ
                     RIIFVTLDDPTGPMDITVFERVQARCARTVFHSWLLLFKGIVRKRGGASRTQRTDPRA
                     VGITVVAQEAFDLAEIAADRKAGHSLAAALGRQRRIQAATGLGPPKPGESRGRRWHSS
                     GGSAGP"
     gene            complement(4222..>4449)
                     /locus_tag="VEV43_14845"
     CDS             complement(4222..>4449)
                     /locus_tag="VEV43_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24844.1"
                     /translation="VAGYDEIVLGGILSSADVLVGAVQEEVLNRVGSAAHVTTQPRLA
                     VARGAIELARSETRPLFEEQLNLSGSRAGTA"
BASE COUNT          770 a         1598 c         1475 g          606 t
ORIGIN      
        1 ctacgacgtc taccaggacc ggacgaaggg ggcctggtgt ctcgctcgga tctgggactg
       61 aggtcgccgg cgccctctgc tcgattcaga agaaattctg aaataaacag agggacgttc
      121 gggacaactg tgggtaaacc ccaaggagtt gcgggaaccc caagcgccgc cggggcgaca
      181 gacgtcgaac gagcttcgcg cgggggccgt gcgggtaggc gggcgcacgg ccccggcgcg
      241 atccccgacg agcgccgggc cgagcccatc ccacccgaac ggaagtatcg tcccgcgcat
      301 gccgaggacc cggtgggacg ccgtgcgcga cggcgacgtg cggatgccgg tgaacgagac
      361 gctcgggttc gagctcgacc gcagcgccga cggcgtcgcc tacgactgga cggtcccctc
      421 ggagtactgc cacggcgcgg gcaacctcca gggcgggatg ctcgcggccc tcgccgacgc
      481 ggtgctgggc ggggtcgcct cgaccgtcct tcccgaggac cgctatcccg tcctcgccga
      541 catgaacctc tcgatcctgc gcccggcccc cgcgggtgcg cggctccgag cgacgggccg
      601 cgtcgtgaag gcgggccgcc gcgtgatctt cacggaggcc gagatcaccg acgcctcgac
      661 gggcaagctg atagcgaagg cgagcggaac ggccctagcc gccgaggcct aaccgcgggg
      721 acgaccgcat gagcggtttc gtccacctac atgcgcacac ggtctggtcg ctgagagacg
      781 gagcgatacc agccgaggtc ctgccgcact tcgccaaaga ccagggctac gaagcaatag
      841 caatgacgga ccacgactcc ctgacaggag cagtccgctt cacgaaagcc tgccgcagcg
      901 caggaatcaa accgatatac ggcgtggaac tgaccgtacg gagggagccg ccggaggacg
      961 cccgcccgga gcgacgacta aaaaacggtg tggaggagcg gagcggcggg cgtccggagg
     1021 cggcgggtac caactggaac ggtctcgatc acgtgacggt gatcgccaag aacgcgaccg
     1081 gctacgccaa cctctgccgg ctcatctccg acgcccacct ctcccacgaa cgagaccgcc
     1141 ccgagaccac acgaacaaag atcgccgagc gggccgaggg actgttcgtc ctctccggct
     1201 gtagccgcgg cgaggtcgcg cgcaaggcga atgccggcga catccccggc gcgatcgagg
     1261 cggcgaagag atggcgagac gagatcggtc ccggctaccg cctcgaggtc ttcgaccatc
     1321 gcggctacgg ccaccgcatg ctccgggacc ggctcctcgc ggtcgcccgg gaatcgaaca
     1381 cgagacccgt cgccacgaac gacgtccact acgcgacccc gcgctacgcc ggcgtccacg
     1441 agctcctcca ctcgatcaag gagatcgtcc cgctctcgcg cacgcacgcg ctccgcccga
     1501 actccgagta ctacctgaag gaccccgccg agatgcgggc cctcttcgca gacgcccccg
     1561 aggccgccga cgagacccgg cggatcgccg acgagtgcga cttcgagctc gacctcggca
     1621 cctaccactt cccggacttc gagctccccg agggcgacct ccccgaatgg gtcctcgaac
     1681 gccgctgcta cgagggcgtc taccgccgct tcgaccggat cacgaacgag gtcgaggacc
     1741 gtctgcagca cgagctccgg atgatcaaca agctcggctg gcccagctac ttcctgatgg
     1801 tcgccgacat cgtcgaccac gtgaagacga agctcggcat caggtgtgcg tgccggggga
     1861 gcgccgccgg ctcgctcgtc tgctacctgc tcgggatctc cgacgtcgac ccggttcgct
     1921 acgacctgct gttcgagcgc ttcatgaaca cgaggcgcga ggagctcccc gacatcgacg
     1981 tcgacgtcga atcgcaccgc cgcgaggagg tcctcgccca cgtcctcgac acctacggcg
     2041 ccgaccagac cgcgatgtgc tgcatggtcg acacgttccg ggcccgcatg gcgatccgcg
     2101 aggtcggcaa ggccctcggc ctgcctccgg aggagatcga cctcgtcgcc aaggccttcc
     2161 cgagggtccg tgcgagtgac atcccgcagg cgatccagcg gctgcccgag ctagcgaaca
     2221 gcaacctgga cgcgggccag ctcgagcagc tcttccggct ctgcatcgag atcgacgggt
     2281 tcccgcgcca cctcgcgctc cacccgtcgg gcgtgatcct gtcggcgaac gacctcgccg
     2341 accgcgtccc gatgcaggag agcttcggcg gcttcacgat gctccaggcc gacaaggacg
     2401 acgtcgagga gctcggccta gtcaagctcg acgtcctcgg cgtgcggatg ctgtcgtcga
     2461 tcgcgcatgc ggtcgccgag atcaagcggg cccgcggcga gaccatcgac ctcgaccggc
     2521 tcccgcacga cgacgacaag acgttcgacc tcgtgcggtc gtcgcggacc ctcggctgct
     2581 tccagatcga gtcgccgggc cagcgcgagc tgctcgcacg gttccagccc ggacgcttcg
     2641 aggacctgat cgtcgacatc tcgctgttcc ggcccggccc ggtgaagtcc gacatggtga
     2701 gcccgttcct cgaacgccgc cacggcttcg aggagacccg ctacgtccac ccgaagctcc
     2761 gcccgatcct cgccgagacc aacggcgtcg tcgtgtacca cgagcaggtc atcagggtca
     2821 tcgccgcggc gacgggctgc tcgctggacg acgccgacta cgtgcggcgc cacctcgacg
     2881 cggaccagcc gggccgcgtg atctgggagg gcgtcgcgca gccgatcgag atcacgccgc
     2941 cggactccag cgtcgagagc tggttcaagg cttcggccgt gcagaacggg ttcacgcgag
     3001 gccaggccga cgagctctgg aaggaggtct tcgccttcgc gtcgttcggc ttctgcaagg
     3061 cccacgcggc ggcgttcgcg ctcccgacct acctgtcgac gtggctgaag gcccactacc
     3121 cggccgagtt cctggcgggg atcctgacgc acgaccccgg catgtacccg aggcggctga
     3181 tcgtcgccga cgcccgccac ttcgggacgc cgatccttcc ggtggacgtg aacgcctcgc
     3241 acgcgaccta ccgggtcgaa cgggccggcg acaaggtcgg gatccggctg gccctgagcg
     3301 aggtccgcga cataacggaa gccgagatcg actcgttgat cgagcaccgt ccctacgaat
     3361 cactcgaaga cctgtggcgg aaggcgacgg tcagccgccc ggtcctggag aacctcgtcc
     3421 acctaggcgc cctcgactcg atcaaaggag cgcgcacccg ccgcgagctg ctgtggcgag
     3481 cggtagacct gacgacgagg acgccggccc agaagagaac ccgccgccgc aagcgcgtcg
     3541 cggtcgagtc tccgccgcag ctggcgttcg acctggagga gccgatcgcc gagacgctgc
     3601 cgggcctccc cgagtactcg gagctcgagg agaccgaggc cgagctcgag atcaccggga
     3661 tcgacgcgcg gcggcacatg ctcgacctct accggccgct gctcgccgag atcggctgcg
     3721 tcccggccga ccgcctcgcg gcccagaaga acgacgcgca ggtctgggtc gccggcgtga
     3781 aggtgtcgag ccagaccccg gcgatccgct cgggacagcg gatcatcttc gtcacgctcg
     3841 acgaccccac gggcccgatg gacataacgg tgttcgagcg ggtccaggcc cgctgcgccc
     3901 gcacggtctt ccactcgtgg ctcctgctgt tcaagggcat cgtgcgaaag aggggcggcg
     3961 cgtcgcggac gcagcgcacc gacccccgtg cggtcgggat cacggtcgtc gcccaggagg
     4021 cgttcgacct cgccgagatc gccgccgacc gcaaggccgg ccactcgctc gccgcggccc
     4081 tcggccgtca gcgcaggatc caggcggcga cgggcctcgg gccgccgaag cccggcgaga
     4141 gccgagggcg gaggtggcac tccagcggcg gcagcgcggg gccctgacag atctaacgga
     4201 gacacggcga ggccaggagt gctacgcagt ccccgcccgg ctaccggaca gattgagttg
     4261 ctcctcgaag agaggtcggg tctcgctacg ggcgagttcg attgctcccc gagctacagc
     4321 gagcctgggc tgtgtcgtta cgtgcgccgc cgatccgacc cgatttagga cttcctcctg
     4381 aaccgctccg acgaggacat cggccgaact caatatcccg cccagcacta tctcgtcgta
     4441 ccccgcaac
//
LOCUS       DASQDO010000540         6534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_791267, whole genome shotgun sequence.
ACCESSION   DASQDO010000540 DASQDO010000000
VERSION     DASQDO010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791267"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VEV43_14850"
     CDS             <1..922
                     /locus_tag="VEV43_14850"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HYP24845.1"
                     /translation="GGQYEKHVEMWMDHYLKGVDNGIDKLPSVISQTSDYEGALGFKK
                     GMPKVRNIELIAQESFADKHYNWKLLPSEPVLFPGKPPGVAAFPSAGINTESHMNHHA
                     RSHHDWWWFETPMLDRDVRIFGTPKVRLWSTVKRRWVPITPVLVDVEPAAHYNYGGNH
                     TTYCDPNAVQAGDANQCTEAMVGVTRGFLDSRYRNGLDKEVEVTPGESFGATIVMKPQ
                     DYVFRKGHVIGLQIATEILEWHVPKAPATCEGADPATPDDCAMFQIDWTESQTRLSLP
                     IVDPPRDLDRLFHVHLDDDEECDLPDPLCN"
     gene            957..2177
                     /locus_tag="VEV43_14855"
     CDS             957..2177
                     /locus_tag="VEV43_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HYP24846.1"
                     /translation="MDRQQLVEKYADLTVRAGCNLQPGQRLLVETVVEHAPFARAVAR
                     AAYAAGARHVEVSYGDQHVRKAMIEHAPDDVLTWTPPHLLQQAKDLAEDRAASVWIVG
                     DPEPELFSDLDPARVGKARMLELREESTKQVNERTVAWAIVAYPNEGWAEAVFGEPDL
                     DRLWDAVARAIRLYEDDPVTAWREHVATLGARADALNELGLDGLRYTGPGTDLTVGLL
                     PGSRWMSARFETAWGQPHIPNIPTEEVFTTPDRRRTRGTVRSTRPLVLEAEGVIVNDL
                     EMRFDGGRAVEVNASSGADVMRTQMDTDDNGRFLGEVALVDKASAVGATGVTFKNTLF
                     DENATSHIAIGAGFTFAVEGAGSLSPDEQVEAGVNYSKIHTDFMIGGPEVTIEGVTKD
                     GKTIPIIVDDTWQL"
     gene            complement(2184..3299)
                     /locus_tag="VEV43_14860"
     CDS             complement(2184..3299)
                     /locus_tag="VEV43_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24847.1"
                     /translation="MERIELELRELMTERAAGATEVPGMRPTLRRARRRQAATVVLAA
                     SVAVAVAGGVVGARTVLRDEPAPRIAAPTEEGTYSFRSTPGTAPVVATGEFRGASWEL
                     TATSLEDDIGSVELALVVTRDGVEARERVGVYPGDSAVAAQTLLETDLLDGASAVFGA
                     VIPEVAGVDVRVAGRDDVAISAHRFRGERRLGSVDYYLAFVPGGSQGFVHARDELGID
                     LGTSGYGGAQVSPRIVSSGTAGDERWTLDFAGGVGEGGRDCFQFSSETDTGTICLTPE
                     DALADEPMAMLVFEPAGALGIVGIISDDVGYVRLVTDEGAEHDLPWSQAPASHRDEWP
                     VRIVTAGLDPAASGRFLVYDGAGELIDEQPFTGTPRE"
     gene            complement(3287..3823)
                     /locus_tag="VEV43_14865"
     CDS             complement(3287..3823)
                     /locus_tag="VEV43_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24848.1"
                     /translation="MTAHQGTADGSELRSDAAGRLERMYATHIAAAVRLAFLITGDGE
                     QARDIAQDAFVRVAARFAELRDPEAFPAYLRTAVVNRSRDHLRKLRTHRRFLARHPRR
                     EAVSSQPDVEGKDALWKVLQTLPVRQRTALVLRYYEDLSERQIAHELGTTESAVKSLL
                     TRGLGQLRGELRGETWSG"
     gene            complement(3867..4232)
                     /locus_tag="VEV43_14870"
     CDS             complement(3867..4232)
                     /locus_tag="VEV43_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYP24849.1"
                     /translation="MDLVAQAKAEVENLTPDQVAAELESGDALLVDLREPAEREAASI
                     PGTVHVPRGMLEFAADPDLPAHKPELQREKRVILHCASGGRSALAAKTLKDMGYSNVA
                     HLEGGINRWAQEGKPVDTA"
     gene            4289..4654
                     /locus_tag="VEV43_14875"
     CDS             4289..4654
                     /locus_tag="VEV43_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYP24850.1"
                     /translation="MVGRWHSLVIDCPDPRRLATFYERLLGLERVEDAEDWVTLEGDV
                     TIAFQRVDSFVAPRWPSPEAPQQMHVDVLVDDLDAAEPAVLELGATLLEGSDKPIGFR
                     VYADPDGHPFCLVTPESLS"
     gene            complement(4647..5564)
                     /locus_tag="VEV43_14880"
     CDS             complement(4647..5564)
                     /locus_tag="VEV43_14880"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HYP24851.1"
                     /translation="MPISTVEKIWMNGELVAWEDAKIHVLSHALHYGTGVFEGVRCYE
                     TARGPAIFRLRDHLARMERSARIFMMDIPYAIDELVEATRELVRINDLRSCYVRPIAY
                     RGYGEIGVNPEGNPVDVCIAVWPWGAYLGEEALTKGVRMTVSSWRRHDPNIIPPQAKV
                     TGAYINSVLAKVEALDKGFDEGIMLNPQGYISEATGENLFIVRDGELLTPPLAAGPLP
                     GITRDSVIAIARDLGITVFETMLTRADLYLAEEMFCVGTAAEVTPVREVDGRVIGDPG
                     PVTQALQQRYFQIVKGEDDKYAEWLDYVS"
     gene            complement(5577..>6534)
                     /locus_tag="VEV43_14885"
     CDS             complement(5577..>6534)
                     /locus_tag="VEV43_14885"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYP24852.1"
                     /translation="IVGVEELKACRPGVRVVNTARGGIIDESALVEAVRNGTVAGASD
                     DVFDGEPVTEHPLFSLPNVVVTPHLGASTAEAQDKAGMAIAEQVLLALRGDFVPFAVN
                     IDLGPDIPEPVRPYVQLAERLGRVAVALSGSGVESLRFEYHGGLAEQDTRALTLSGLK
                     GAFSAVVHEPVTFVNAPLLAKERGIAIEESKSSQSLDYVNLIEVRASSEDETVSVAGV
                     LVGKRDTERLVRVYGYDLDMAFAPVMAFFRYVDRPGVVGIVGTLLGEASINIANMQVG
                     RQTVGGEALMGLAVDSPIPQDVLEGIVSKAGLRDARLITVET"
BASE COUNT         1023 a         2295 c         2242 g          974 t
ORIGIN      
        1 cggcgggcag tacgaaaagc acgtcgagat gtggatggac cactacctca aaggcgtcga
       61 caacggcatc gacaagctgc cgagcgtgat cagccagacg tcggactacg agggtgcgct
      121 gggcttcaag aagggcatgc ccaaggtccg caacatcgag ctgatcgccc aggaatcctt
      181 tgccgacaag cactacaact ggaagctgct gccgtcggag ccggtgctct tccccggcaa
      241 gccgcccggc gtcgcggcct tcccctccgc gggcatcaac accgagagcc acatgaacca
      301 ccacgcccgc agccaccacg actggtggtg gttcgagacc ccgatgctgg atcgcgacgt
      361 gaggatcttc ggcacgccga aggtcaggct gtggtcgacg gtcaagcgca ggtgggtccc
      421 gatcacgccg gtattggtcg acgtcgagcc ggccgcccat tacaactacg gcggcaacca
      481 cacgacctat tgcgacccga acgcggtgca ggccggcgac gcgaaccagt gcaccgaggc
      541 gatggtcggt gtcacccgcg ggttcctcga cagccgctac cggaacggcc tcgacaaaga
      601 ggtcgaggtc acaccgggcg agtcgttcgg agcgacgatc gtcatgaagc cgcaggacta
      661 cgtgttccgc aaggggcacg tcatcgggct gcagatcgcg acggagatcc tcgagtggca
      721 cgtgcccaag gcaccggcca cgtgcgaggg ggccgacccg gccacgccgg acgactgcgc
      781 gatgttccag atcgactgga cggagtcgca gacgcgtctg tcccttccga tcgtcgatcc
      841 gccacgagac ttggaccggc tgttccacgt gcacctcgac gacgacgagg agtgcgacct
      901 gccggatcca ctctgcaact gacgggccgg cccggggccg gtcgtatggt ctgccgatgg
      961 acagacaaca gctcgtagag aagtacgcag acctcaccgt acgcgccggg tgcaacctcc
     1021 agcccggcca gcgtctgctc gtagagaccg tcgtagagca cgctccgttc gcacgtgccg
     1081 tcgcgcgcgc cgcgtacgcc gccggcgcgc gtcacgtcga ggtgtcgtac ggcgaccagc
     1141 acgtgcgcaa ggcgatgatc gagcacgcgc ccgacgacgt gctgacgtgg acgcctccgc
     1201 acctgctgca acaggcgaag gacctcgcag aggacagggc cgcttccgtg tggatcgtcg
     1261 gcgatcccga gccggagctg ttctccgacc tcgacccggc ccgcgtcgga aaggcccgga
     1321 tgctcgagct gcgcgaggag tcgacgaagc aggtgaacga gcgcacggtc gcgtgggcga
     1381 tcgtcgccta tcccaacgag gggtgggccg aagcggtgtt cggcgagccg gacctggaca
     1441 ggttgtggga cgccgtcgcg cgcgccatcc gtctctacga ggacgacccg gtgacggcat
     1501 ggcgcgagca cgtcgcgacg ctcggggcgc gggcggatgc gctgaacgag ctggggctgg
     1561 acggcctgcg ttacacgggg cccgggactg acctcaccgt cgggctgctc cccggaagcc
     1621 ggtggatgag cgcgcgtttc gagacggcgt gggggcagcc ccacatcccg aacatcccga
     1681 cggaggaggt cttcaccacg cccgaccgcc gtcgaactcg agggaccgtg cgatccaccc
     1741 gtcccctggt cctcgaagcc gagggcgtga tcgtgaacga cctcgagatg cgcttcgacg
     1801 ggggcagggc cgtcgaggtg aacgcgagct cgggcgccga cgtcatgcga acgcagatgg
     1861 acacggacga caacggccgc ttcctcggcg aggtcgcact ggtcgacaag gcgtccgcgg
     1921 tgggcgcgac cggggtgacg ttcaagaaca cgctgttcga cgagaacgcg acctcgcaca
     1981 tcgcgatcgg ggccggcttc acgttcgcgg tcgagggggc gggttcgctg agccccgacg
     2041 aacaggtgga ggccggggtg aactattcga agatccacac ggacttcatg atcggggggc
     2101 cggaggtgac gatcgagggc gtcacgaagg acggcaagac gatcccgatc atcgtcgacg
     2161 acacttggca gctgtagccg gcgttactcg cggggcgtcc ccgtgaacgg ctgctcgtcg
     2221 atcagctcgc ccgctccgtc gtagaccagg aaccgtccgg aggcggcggg atcgagcccg
     2281 gccgtcacga tgcgcaccgg ccactcgtca cgatgggagg cgggggcctg cgaccacggc
     2341 aggtcgtgct cggccccctc gtcggtcacg agccgcacgt agccgacgtc gtccgagatg
     2401 atcccgacga tgcccagcgc ccccgcgggc tcgaagacga gcatcgccat cggctcgtcc
     2461 gccagcgcgt cctcgggggt gaggcagatc gtgcccgtgt cggtctcgct ggagaactgg
     2521 aagcagtcgc ggcccccctc gcccaccccc ccggcgaaat cgagcgtcca cctctcgtcg
     2581 ccggccgttc cgctcgacac gatccggggc gagacctgcg ctcccccgta cccggacgtc
     2641 ccgagatcga tcccgagctc gtctcgggca tgcacgaaac cctgtgatcc gccggggacg
     2701 aacgcaaggt agtagtcgac ggagcccagg cgtcgctcgc ctcggaaccg gtgggcggag
     2761 atggcgacgt cgtccctgcc ggccacccgc acgtcgacac cggcgacctc ggggatcacc
     2821 gctccgaaga ccgccgacgc gccgtcgagg aggtccgtct ccaagagggt ctgtgcagcg
     2881 acggcagagt cacccgggta cacgcccacg cgctcacgtg cctcgacacc gtcccgcgtc
     2941 acgacgagtg caagctcgac cgacccgatg tcgtcctcga gcgacgtggc ggtgagctcc
     3001 cagctcgcgc cgcggaactc gcccgtcgcg acgaccgggg ccgtccccgg cgtggaccgg
     3061 aacgagtacg tcccttcctc cgtgggggcc gcgatgcgcg gagccggctc gtcgcgcagc
     3121 accgtccgtg cgccgacgac cccgcccgcg acggccaccg cgacggacgc cgcgagcacg
     3181 accgtcgcgg cctgccgccg gcgcgcccgg cgcagcgtcg gtctcatccc gggcacctcc
     3241 gttgcgccgg ccgcccgctc cgtcatcagc tctctcagct cgagctctat ccgctccacg
     3301 tctcacctcg cagctcgccg cgtagttggc ccagcccgcg cgtcagcagc gacttcacgg
     3361 cggactcggt cgttcccagc tcgtgagcga tctgacgctc ggacaggtct tcgtagtagc
     3421 ggaggactag cgcggttcgc tggcggaccg gcagcgtctg gagcaccttc cacagcgcgt
     3481 ccttgccttc gacgtcgggc tgcgacgaga ccgcctcgcg ccgcggatga cgcgcgagga
     3541 acctccggtg cgtccgcagc ttcctgaggt gatcccggct gcggttcacg accgcggtcc
     3601 gcaggtatgc ggggaaggct tcggggtcgc gcagctcggc gaacctcgcc gcgaccctca
     3661 cgaacgcgtc ctgcgcgatg tcgcgggcct gctctccgtc gcccgtgatc aggaacgcga
     3721 gccggaccgc cgccgcgatg tgcgtcgcgt acatgcgctc gagcctgccc gccgcgtcgc
     3781 tgcggagctc ggatccgtcg gcagtcccct ggtgtgcggt catacgcccc ttaggacgtg
     3841 acgggtaccg ggttggtcgc gctgggtcag gcggtgtcta ccggcttgcc ctcctgggcc
     3901 caccggttga tcccaccctc gaggtgggcg acgttcgagt agcccatgtc cttcaacgtc
     3961 ttcgcggcga gggccgaacg tccgcccgac gcacagtgca ggatcacgcg cttctcgcgc
     4021 tggagctcgg gcttgtgggc cgggaggtcc gggtcggcgg cgaactcgag catcccgcgc
     4081 gggacgtgga ccgtccccgg gatcgacgcc gcctcacgct cggcgggctc gcgcaggtcg
     4141 acgaggaggg cgtcgccgct ctcgagctct gccgccacct ggtcgggggt gaggttctcg
     4201 acctctgcct tcgcctgcgc tacgagatcc atggctcctt gtcccacgct gcctcctcgg
     4261 gtcgcgttcg gatgcgatgc tcgtgggcat ggtaggccgc tggcactccc tcgtgatcga
     4321 ctgccccgac ccgcgccgcc tcgcgacgtt ctacgagcgg ctcctggggc tcgagcgggt
     4381 cgaggatgca gaggactggg tgacgctcga aggcgacgtc acgatcgcgt tccagcgcgt
     4441 ggactctttc gtcgcgccgc ggtggccgag ccccgaggcc ccccagcaga tgcacgtcga
     4501 cgtgctggtg gacgacctgg acgcggcgga gcctgcggtg ctcgagctgg gggcgacgct
     4561 gctggaggga tcggacaagc cgatcgggtt ccgggtctac gcggacccgg acgggcaccc
     4621 gttctgcctg gtgacgccgg agagcctcag ctgacgtagt cgagccactc cgcgtacttg
     4681 tcgtcctcgc ccttcactat ctggaagtag cgctgctgca gggcttgcgt caccgggccc
     4741 gggtccccga tcacacgccc gtcgacctca cgcaccggcg tcacctcggc cgccgtgccg
     4801 acgcagaaca tctcctccgc caggtacagg tcagcccgcg tcagcatcgt ctcgaagacc
     4861 gtgatcccca ggtcgcgcgc tatcgcgatg accgagtcgc gggtgatccc gggaagcggc
     4921 ccggccgcga gcggcggcgt caggagctcg ccgtcgcgga cgatgaacag gttctcgccc
     4981 gtcgcctcgg agatgtagcc ttgggggttc agcatgatcc cctcgtcgaa gcccttgtcg
     5041 agcgcctcga ccttggccag caccgagttg atgtacgcgc cggtcacctt cgcctgcggc
     5101 gggatgatgt tcgggtcgtg ccgccgccac gaagagacgg tcatgcgcac gcccttcgtc
     5161 agagcctcct cgccgaggta cgccccccac ggccacaccg cgatgcacac gtcgacgggg
     5221 ttcccctccg ggttgacgcc gatctcgccg tagccccggt acgcgatcgg gcgcacgtag
     5281 cacgacctca gatcgttgat ccggacgagc tcacgcgtcg cctcgacgag ctcgtcgatc
     5341 gcgtagggga tgtccatcat gaagatgcgg gccgaacgct ccatgcgcgc gaggtggtcg
     5401 cgcagccgga agatcgccgg gccgcgggcg gtctcgtagc aacggacccc ctcgaacacg
     5461 ccggtcccgt agtggagcgc gtggctgagc acgtggatct tggcgtcctc ccacgcgacg
     5521 agctcgccgt tcatccagat cttctcgacg gtgctgatcg gcatgcgcgt gagctcctag
     5581 gtctcgaccg tgatgagccg ggcgtccctc aggccggcct tcgaaacgat gccctcgagg
     5641 acatcctgag ggatgggcga gtcgaccgcc agacccatga gggcctcgcc cccgacggtc
     5701 tgccgtccga cctgcatgtt cgcgatgttg atcgacgcct cgcccaggag ggtgccgacg
     5761 atgccgacga caccggggcg gtcgacgtag cggaagaacg ccatcaccgg cgcgaacgcc
     5821 atgtcgaggt cgtagccgta gacacggacg agccgctcgg tgtcgcgctt gccgacgagg
     5881 acgccggcga ccgagaccgt ctcgtcctcg gacgacgcgc gcacctcgat caggttgacg
     5941 tagtccagcg actgcgacga cttggactcc tcgatcgcga tcccccgctc cttcgcgagc
     6001 aggggcgcgt tgacgaacgt cacgggctcg tgcacgactg ccgagaacgc gcctttgagg
     6061 cccgagagcg tgagcgcccg cgtgtcctgc tcggcgaggc ccccgtggta ctcgaaccgg
     6121 agggactcga cgcccgagcc ggagagcgcc accgcgacgc gacccagtcg ctccgcgagc
     6181 tggacgtagg gccgcacggg ctcggggatg tcggggccga ggtcgatgtt gacggcgaag
     6241 ggtacgaagt cgccgcggag cgcgagcagg acctgctcgg ctatcgccat gccggccttg
     6301 tcctgggcct ccgcggtcga agcaccgagg tgcggcgtca cgacgacgtt cgggagcgag
     6361 aacagcgggt gctcggtgac gggctcgccg tcgaagacgt cgtccgacgc ccccgcgacc
     6421 gtgccgttgc ggactgcctc cacgagcgcg gactcgtcga tgatcccgcc gcgcgcggtg
     6481 ttcacgaccc tgacgccggg cctgcacgcc ttcagctcct cgacccccac gatc
//
LOCUS       DASQDO010000541         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_179234, whole genome shotgun sequence.
ACCESSION   DASQDO010000541 DASQDO010000000
VERSION     DASQDO010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179234"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1971
                     /locus_tag="VEV43_14890"
     CDS             175..1971
                     /locus_tag="VEV43_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24853.1"
                     /translation="MTQQADAASTDGTTEEPVENQGSETGDGEADAPVRTRAIVYVPH
                     LGHTPGRSFNDFATRLALVLNLSDPAASVRYSVKDEGETVRFGAATTKTVAPGVTIVR
                     EDGAAASAIGVYEYAYSDSFSRRFKAQRASAKVANLLLTVFVNLARLARSFTKGWRPD
                     GARWLVWGVPLNLALLVITAAYLIFDPEEWWASNAVGATAAISAAFALMAAARLSSLG
                     AGDEAGANGSPEDEALPEDPEGSEGPEGSGAQSRRIRGAVSFGAAAVIVGAMLAADPW
                     RTWRADVIGEVLLIAGLLWLFGAWLTLSSSDVGHRIQLGIGFAALGLMSLDAVLVAAA
                     ALAQGATLLTEGETVDALAGYLAGVAVVLTTVGISVTPKEVSGHVNNAAGELVAAVRY
                     LSEGYGRDAQTGRLYSLVCQLAESGYTAVDIVGYSFGSILALDTLYRIAPAEVPQGPA
                     DVIDRLVTIGSPVVTIGTFWPGYWEGRSPWGGDWINVWSPLDVLSSNLTGAPTVQNDG
                     RCIAPCGEDGEILLPTERVYATTTEKLSAWQKLALRGLRAHSEYWATPFDEDPGAIDR
                     IGDLWAGTDWNGASEDPAGAPPDSGDPVPATT"
     gene            2004..2570
                     /locus_tag="VEV43_14895"
     CDS             2004..2570
                     /locus_tag="VEV43_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24854.1"
                     /translation="MRRMSVVLLVAAMLVGATGAVADTRRWRDGNEARGPLDIARIAQ
                     GHRIGPKGVAQVVHTIRLHRAWPVKRLRHRGYVVVQFDRRGHPDGPNERMLRIFYKRG
                     GLVATMYNSLGDPPKRLARVALWRPDRRTVKVAFPPKLLKRRGLERYEWNVMSFVEGR
                     HDLCAKRHGCMDLAPNRENGRRYVRHVL"
     gene            complement(2567..>3168)
                     /locus_tag="VEV43_14900"
     CDS             complement(2567..>3168)
                     /locus_tag="VEV43_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24855.1"
                     /translation="EPLPVPAPGPSVAPVTTRRFELPVLGDFSVSPALPDTELTTVRS
                     PRLRAGKGSVAFFAVARPRVPVRCIAQVRLGLPVEGWTDSAGEELAVYPSQVVNAFRK
                     RNGDRYGYSGSLLDVRPRATFVGHVEGWAEWDVTAIVKRWVGGVFPSRGMRVPRKGPV
                     VLALRDADGAEPLVRVAVGSVEAPRPPAVVARVEKECPG"
BASE COUNT          493 a         1099 c         1122 g          454 t
ORIGIN      
        1 gttcgagccg gcgaacccgg cgctgcagcc gttcgaggcc ccgcagggaa ccgagatcgt
       61 agggaaggta gtggcggtct tccgcaagat gtagccgcgc ccaccgaacc ccgttgacgg
      121 aaacgccggc cgggacgcag agttgacccg gcggtcgacg ggggtggggg cgcgatgacg
      181 cagcaggcgg atgctgctag cacggacggc acgacagagg agcctgtcga gaaccagggt
      241 tcggaaacag gcgacggcga ggccgacgcg ccggtgcgga cgcgcgcgat cgtgtacgtg
      301 ccgcacctgg gccacacacc cgggcgatcc ttcaacgact tcgccacgcg actggccctg
      361 gtcctcaacc tgagcgaccc cgcggcatcg gtcaggtact ccgtgaaaga cgagggcgag
      421 accgtccgct tcggggccgc cacgacgaag acggtcgcgc cgggtgtcac gatcgtacga
      481 gaagacggag ccgcggcgag cgccatcggc gtctacgagt acgcgtactc ggactcgttc
      541 tcccgtcgct tcaaggctca gcgcgcgtcg gcgaaggtgg cgaacctgct gctgacggtc
      601 ttcgtcaacc tcgctcgcct tgcgcgctct ttcacgaagg ggtggcggcc cgacggagcg
      661 cgctggttgg tctggggcgt cccgctgaac cttgccctcc tcgtgatcac agccgcgtat
      721 ctgatcttcg accccgagga atggtgggcg tccaacgccg tgggggccac cgcagcgatc
      781 tctgcggcgt tcgcgctcat ggccgccgca aggctgtcga gcctcggggc cggtgacgaa
      841 gccggcgcga acggcagccc cgaggacgag gccctgcccg aggacccgga agggtccgaa
      901 ggtccggaag ggtccggggc gcaatctcgg cggattcgcg gagccgtgtc gttcggcgcc
      961 gcggcggtga tcgtgggcgc aatgctcgcc gccgacccgt ggcgcacgtg gagagccgac
     1021 gtcatcggtg aggtcctcct gatcgcgggc ctcctgtggc tcttcggggc gtggctgacg
     1081 ctgtcgagct ccgacgtggg acaccgcatc cagctcggca tcggattcgc cgccttggga
     1141 ttgatgtctc tggacgccgt cctcgtggcg gcggcagccc tcgcgcaggg tgcgacgctg
     1201 ctgacggaag gagagaccgt cgacgctctg gccgggtacc tagccggtgt cgccgtcgtg
     1261 ctcacgaccg tcgggatcag cgtcacgccc aaggaggtgt cggggcacgt gaacaacgcc
     1321 gcgggagagc tcgtagccgc cgtccgatac ctgtcggagg gatacgggcg cgacgcccaa
     1381 acggggcggc tgtactcgct cgtctgccag ctcgccgaat ccgggtacac ggccgtcgac
     1441 atcgtcggtt acagcttcgg gtcgatcctg gcgctcgaca cgctctatcg catagcgccc
     1501 gccgaggtcc cacagggacc cgcagacgtc atcgaccggc tcgtcacgat cggctcgccc
     1561 gtcgtgacca taggcacctt ctggccgggt tattgggagg gccggtcgcc ctggggcggc
     1621 gactggatca acgtctggtc gcccttagac gtgctctcgt ccaacctgac cggtgcgccc
     1681 acggtgcaga acgacggccg ttgcatcgct ccgtgcggcg aagacggcga gatcctcctg
     1741 cccaccgaga gggtctacgc gacgacgacc gagaagctct cggcgtggca gaagctcgcc
     1801 ctgcgcggcc tccgtgccca ttccgagtac tgggcaactc cgttcgacga agacccaggc
     1861 gcgatcgatc gcatcggaga cctgtgggca ggaaccgatt ggaatggcgc gtccgaagat
     1921 cctgccggcg cgccgcccga ttccggcgac cccgttccgg ccacgacgtg agctcggacg
     1981 tgcggcgtgg gatcatccgc cgcatgagac gcatgtcggt cgtcctcttg gtcgctgcga
     2041 tgctcgtcgg ggcaaccgga gcggtggccg acacgcggcg gtggcgggac gggaacgagg
     2101 cccgcggccc cctcgacatc gcccggatcg cccagggcca ccgcatcggc ccgaagggcg
     2161 ttgcgcaggt cgtccacacg atccggctcc accgggcctg gcccgtcaag aggctccgcc
     2221 accgcggcta cgtcgtcgtc cagttcgaca ggcgcggcca tcccgacggg ccgaacgagc
     2281 ggatgctgag gatcttctac aagcgcggcg ggctcgtcgc gaccatgtac aactcgctcg
     2341 gcgaccctcc caaacgcctc gcgcgggtcg cgctctggcg gccggaccgg cgcaccgtca
     2401 aggtcgcgtt cccgccgaag ctgctcaaga ggcgggggct cgagcgctac gagtggaacg
     2461 tcatgtcgtt cgtcgaaggc cggcacgatc tgtgcgcgaa gcggcacggt tgcatggacc
     2521 tcgcgccgaa tcgcgagaac ggccgcaggt acgtcaggca cgtcctctag ccgggacact
     2581 ccttctcgac gcgcgcgacg accgcgggtg ggcgcggtgc ctcgaccgag ccgacggcga
     2641 ccctgaccag cggttcggcg ccgtcggcgt cgcgcagcgc cagcaccacc ggtcccttgc
     2701 gagggacacg catgccgcgc gacgggaaga cgccgccgac ccatcgcttg acgatcgcag
     2761 tgacgtccca ctcggcccat ccctcgacgt ggccgacgaa ggtcgcccgc ggccgcacgt
     2821 ctaggaggga gccggagtag ccgtagcggt caccgttgcg cttgcggaac gcgttcacca
     2881 cctgcgacgg atagaccgca agctcttcgc ccgcgctgtc cgtccatcct tcgaccggga
     2941 gccccagccg cacctgggcg atgcacctca cgggcacgcg cgggcgcgct acggcgaaga
     3001 acgccacgct ccccttgccg gcgcgcagcc tcggtgaccg gaccgtcgtc agctccgtgt
     3061 cgggcagcgc cggcgagacc gagaagtcgc cgagcaccgg aagctcgaac cggcgggtcg
     3121 tgacgggggc gaccgaaggc cccggcgcgg gcaccgggag gggctccg
//
LOCUS       DASQDO010000542         9752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_913739, whole genome shotgun sequence.
ACCESSION   DASQDO010000542 DASQDO010000000
VERSION     DASQDO010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913739"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VEV43_14905"
     CDS             <1..1516
                     /locus_tag="VEV43_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24856.1"
                     /translation="GASLPWGEGPRDVAAASVIKSDVRAAALELGAYDARVAIIERGG
                     HPDVDAREMTARVRFEAPERFRLEVRDHTSYPEGRWPSNDVTVVANGRRWWMAETTSC
                     PTPALPACSLVVRERRGIEGRQPFDGTTALPTDIVVPLETIASSGGFEVSARETVLGR
                     RAHRIVLDYRQALPLVTSIQAGGSWRTFAATDRVDVWLDAETSFPLRFTVTRPGRPPL
                     LDVRAQSFSVPDGFGPGTFSVPRRGAFKPGGWDEEPAAELPSGLAPGYTAGLVPYRSG
                     TTADGKTMLSYARGMTWLKVSSAPAERADLGHLLTAEEIRTPSGWAYYQPADASLKRR
                     VEIFDSGLHVQLESNLSRDDLLRVAGSLGVDGTKIPPQVTTRKGAVIERVDAAEALRR
                     APFARRPGYVPRGYSPATVLLSHSLRETSVSFFFRHAEAEYGGSGIQVTQSKPAGLLT
                     PTSETPVVVPVGEVDARWFAERGVLEWIEGGVHVAVTVPSGDLGTATAVAEGLR"
     gene            1513..2748
                     /locus_tag="VEV43_14910"
     CDS             1513..2748
                     /locus_tag="VEV43_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYP24857.1"
                     /translation="MTPRPALARVAWGALGALACGAVILGADGVGRERRPSTTPAPIV
                     VTEAPAKSAPVVMPRPSGKVLLAWATGDGLPARTEAVLEGVRGVSAVTVVHAGIDWLH
                     ASFDADGSLLDRPKPPMAIPMEVAAVDPADFASFVPAGERDLVLALGPRDVLLPETSA
                     ELRRTPPGSVLRLEAGRFTVSGVVSDAAAGGYEAIVSESAPKQWPSTETYALIRTKSP
                     RLRSSVRKTITSLLGPGDQLQMRAEGENPFLRYGDAVLPQLIVKSVFGEFAATPRADG
                     FLDVDPAWRKANVKTARVPLLGDVTCHRALFPQLRRALTRAVGSNLSFAIDPADFGGC
                     YSPRFINRNPAGRISHHSWGIAVDLNVTENAYGTKADLDLRLVRVMESAGFTWGGRWV
                     VPDGMHFEWSEFPGPSPKG"
     gene            2976..4430
                     /locus_tag="VEV43_14915"
     CDS             2976..4430
                     /locus_tag="VEV43_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24858.1"
                     /translation="MSSALQLDEEKVRRWLGLLRHPNRLDSPEVRALLRAHGRLPADA
                     TPLDVGRAAARFLTDTIDRLAAPPDASAGERLPHAVLRTCFVDGAKLYQAAAKLGLSE
                     RQLTRERGRAISLLTAQLSLGPLRSRFVPEEIPTIDDFVPRADLVAAITETLGEGRSA
                     RVHGPPGIGKTSLVAEVAIAESRRSAVWWYRFRRGVNDTLHAVLFELGQWLGSQGLTD
                     LATYVTPNVAALDSAVASRIALRALADTNALVVLDDYQTVEDDLPISGFFDEALVRTP
                     TLRLITSSRHRPGTAHARAAIEVPPFRLEQTERLLLHAGTRRPRETIAKIHAWTGGNP
                     HLVKLSASWLKSATPAEIARGVEALIDREEVQAFLLSNITELLDSDDRGILEAASIFR
                     GRFSDDALAYVARQTRGAVQDASHRLVRSYVATRSRQGVSAFFHGSVRDYVYERLDAE
                     VRSGLHLRAAAWFHRLGDDEETAYHRDRGGLEGG"
     gene            complement(4427..4756)
                     /locus_tag="VEV43_14920"
                     /pseudo
     CDS             complement(4427..4756)
                     /locus_tag="VEV43_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            complement(4818..5426)
                     /locus_tag="VEV43_14925"
     CDS             complement(4818..5426)
                     /locus_tag="VEV43_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYP24859.1"
                     /translation="MPRRRRMSQDELVRVGRRLRELRLAAGMTQAELAGRRFSHAYVS
                     VLEAGRREPSRAAVDYFAQRLGVAVEELWSEKGASWALQMAEDLRAEGLHGESRELLA
                     RTLGNLERDRELHPRVLVVLHRELARIDIDGDPASAATHLQRCLELAGDDDSMLAERA
                     EAHAGLGDLLVREGKTSEALEQYRIASSLLLELVGRRPSLLT"
     gene            complement(5433..7682)
                     /locus_tag="VEV43_14930"
     CDS             complement(5433..7682)
                     /locus_tag="VEV43_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP24860.1"
                     /translation="MTGPRRSEVALYQRNARHVTDRRVLHEIEKGEAIAALPWWKRRA
                     GRAEHERDWAEQETVAAAAAEPALARLRDAATEFDSALRRLAALDSAELRAKVEPEIQ
                     RVAAVSTDLSEALAALERRDFGAAGAAADRAGRELWLVGGPLVVALNEALGRATIGRA
                     QRDALLDNISERQRAKAALEQARLAGPAASMEAAATVHLVEAAGLRLAAEVLAVQAQP
                     KQAPARSAGSSGPAIVPPHELETFDDVAGLHEVKEQLRATVGTILENPDEAARYRLVH
                     NGILFYGPPGTGKTLLSRALAGEYGLRYLRVTPATIGSPLAHETAANVQRVFDLAIAS
                     APCLLFLDEVDSLAGARDEAPGSEHREVVTQLMTSLETCRSTPGLLVCAATNDVDRLD
                     PGLREGRFDARIRVPLPDREARREIVLLHLKKRTDSVDWDAVDVDAVVDATGGRNAAA
                     LEQIVTLAAQAALASSRPISGSDLLTAVRHGSGRDRAVLEERVTWDDVVLPDDTREQL
                     MDLVNVFVKPDLARSLGIEPPPGILLHGPPGTGKTTIARAMASEIDASFYEHSAADLL
                     SKWAGESEQRVAKLFAKARANRPSIIFVDEIDGLLRARGGDSANRWEERVVSQFLREL
                     DGLATGGGVLLVGATNRPDVVDPAVRERRLAPVEIGLPDAAGRLQLLRRLLASASVAP
                     DVDLRELALATEGMSGADLKRLRDLAGMKTLTRASREGTGAANVRIASEDLTVALQGM
                     RRRASLAVV"
     gene            complement(7679..9658)
                     /gene="ftsH"
                     /locus_tag="VEV43_14935"
     CDS             complement(7679..9658)
                     /gene="ftsH"
                     /locus_tag="VEV43_14935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYP24861.1"
                     /translation="MPFGGSLAPRARRALSSWLLRRRRARAGRKKTGSRRLLVVLTAS
                     LVALLGLFAAALAYLRPPAHGHELTLDELSSLASEGRLEEARFLDADATITGRYSGGA
                     GEPGGSGEFFLAYPSSDAVTAFLVETTTEAGARVSIDPQPSKDVIGTVSTFLLPLMIL
                     ANLFALLFSASRGGSAGIGEVMTFGTIGDRRRRRGGAAPVTFDQVAGADEAVAELREV
                     VDYLKDPARYEEVGALPPKGVLLFGPPGCGKTLLAKAVAGEAGVPFFSVAGAEFVESL
                     VGVGAARVRDLFQRVRAVAPAIVFIDELDAAGRKRAAGGGSGGADEREQTLNQLLVEM
                     DGFELSAGIVVIGATNRPDILDPALLRPGRFDRHVTVEQPDPAGRLEILELHAAGKPV
                     SPLADLTVIARRTPGFSGADLANVLNEAALLTVREGKPEIEMAELEEAVQRVLAGPKK
                     KGRVLSPEERKRAAFHEAGHAIVAAASGRLEQVHRVSILARGRSLGTIALQGDDSALQ
                     TRSELVGGIVIALGGIAAEMLLFGEPSTGAEDDLQFATDQAMDVVGRFGMGERRRRLL
                     ARAQDELLGETASLGPVSALTHQEMEAEVDRLIAEAEEEAAALLTRHRDVLGALAERL
                     EAEETIEGPDLESILALVRPEVTLFGGVVAVEGRT"
BASE COUNT         1322 a         3636 c         3442 g         1352 t
ORIGIN      
        1 cggcgcgtcg ctcccgtggg gagagggccc ccgcgacgta gctgccgcgt ccgtgatcaa
       61 gagcgacgtg agggccgcgg cgctcgagct cggcgcctac gacgcgcgcg tcgcgatcat
      121 cgaacggggc gggcatcccg acgtcgacgc gagggagatg accgcccggg tcaggttcga
      181 ggcgcccgag aggttccgcc tcgaggtccg cgaccacacg agctatcccg aaggcaggtg
      241 gccgtcgaac gacgtgacgg tggtcgcgaa cggccgccgg tggtggatgg ccgagaccac
      301 ctcctgcccg acgcccgccc tgcccgcctg ctcgctcgtc gtgcgggagc gcaggggcat
      361 cgagggacgg cagcctttcg acgggacgac cgcgctcccg accgacatcg tggtgccgct
      421 cgagacgatc gcgtcgtcgg gcgggttcga ggtctccgcg cgcgagaccg tcctcggccg
      481 gcgagcgcat cgcatcgtcc tcgactaccg ccaggccctg cccctcgtca cgtcgatcca
      541 ggccggtggg tcgtggcgca cgttcgcagc gaccgaccgc gtcgacgtgt ggctcgacgc
      601 cgagacctcg ttcccgctgc ggttcaccgt gacgaggccc gggcggccgc cgctgctgga
      661 cgtccgggcg cagagcttct cggtccccga cggtttcggg ccggggacgt tctcggtacc
      721 gcgccgcggt gcgttcaagc cgggtgggtg ggacgaggag cccgcggccg agctcccgtc
      781 ggggctggcc cccggctaca ccgccggcct ggtcccctat cgctccggca cgaccgccga
      841 cggcaagacc atgctgagct acgcacgcgg gatgacgtgg ctcaaggtgt cgtcggctcc
      901 ggcggagcgc gccgacctcg gccatctgct gacggcagag gagatccgga cgccttcggg
      961 gtgggcgtac taccagcctg ccgacgcgtc gttgaagcgg cgcgtcgaga tcttcgactc
     1021 gggcctgcac gtgcagctcg agtccaacct gtcgcgagac gacctgctgc gcgtcgcggg
     1081 ttcgctcggc gtcgacggca cgaagatccc gccgcaggtg acgactcgga agggggccgt
     1141 gatcgaacgc gtcgacgccg ccgaggcgct gcgccgggcc cccttcgcgc gccggcccgg
     1201 ctacgtcccc cgcggctaca gcccggcgac ggtcctgctc tcgcactcgc tacgcgagac
     1261 gtcggtgtcg ttcttcttcc ggcacgcgga ggccgagtac ggcgggagcg gcatacaggt
     1321 gacgcagtcg aagcccgccg gcctgctgac accgacctcg gagaccccgg tcgtggtccc
     1381 ggtcggtgag gtcgacgccc gctggttcgc ggagcgcggc gtgctcgagt ggatcgaagg
     1441 cggcgtccac gtggccgtca cggtcccgtc gggcgacctc ggaaccgcga ccgcggtcgc
     1501 cgaaggcctg cggtgacgcc gcggccggca ctggcgcgcg tcgcgtgggg tgccctgggc
     1561 gccctcgcat gcggcgcggt gatcctcggc gcggacggcg tcggccgcga gcgccgcccc
     1621 tcgaccacgc cggctccgat cgtcgtcacc gaggcgcccg ccaagagcgc ccccgtggtg
     1681 atgccgcggc ccagcggcaa ggtcctgctc gcatgggcca cgggcgacgg actgccggcg
     1741 cgtaccgaag cggtgctcga aggcgtgcgc ggcgtctccg ctgtcaccgt cgtgcacgca
     1801 gggatcgact ggctccacgc gtcgttcgac gcggacggga gcctgctcga caggccgaag
     1861 cccccgatgg ccataccgat ggaggtggcg gcggtcgacc ccgccgactt cgcctcgttc
     1921 gtcccggccg gcgagcgcga cctcgtgctc gcgctcggcc cccgcgacgt gctgctgccc
     1981 gagacctcgg ccgagctccg gcgcactccg ccgggatccg tgctccgcct cgaagccgga
     2041 cgcttcacgg tgagcggcgt cgtcagcgac gcggccgccg gcggctacga ggcgatcgtg
     2101 tcggagtccg cgccgaagca gtggccgagc accgagacgt atgcgctgat ccggacgaag
     2161 tcaccccggc tgcggagcag cgtccgcaag acgatcacgt cgctgttggg gccgggtgac
     2221 cagctccaga tgcgcgccga gggtgagaac ccgttcctgc gctacggcga cgcggtcctc
     2281 cctcagctca tcgtgaagtc ggtgttcgga gagttcgccg ccacgccgcg cgccgacggc
     2341 ttcctcgacg tcgacccggc ctggaggaag gcgaacgtca agactgcgcg ggtcccgctg
     2401 ctcggcgacg tcacgtgtca ccgcgcgctg ttccctcagc tccggcgcgc gctcacgcgc
     2461 gcggtcgggt cgaacctctc gttcgcgatc gaccccgcgg acttcggcgg ctgctacagc
     2521 ccgcgcttca tcaatcgcaa cccggccggc cggatctcgc accattcctg gggcatcgcc
     2581 gtggacctca acgtgaccga gaacgcttac ggcacgaagg cggacctgga cctgcgtctc
     2641 gtccgcgtga tggagtccgc gggcttcacg tggggcgggc ggtgggtggt ccccgacggg
     2701 atgcacttcg agtggagcga gttcccggga ccgagcccca aaggttgact cgtgtgacga
     2761 cttgtgatca cacctcgtgt tgaccacacg ctaacgaagg caaacgcgcc tactctcgaa
     2821 cgatctccgg cggctgtgta acaaggcgtc gccaccggct cgccaactac tagccagatt
     2881 ccccgaatac gccgggagcg cggtgtcaca cgacctttac ttccggccat ggtgcggacg
     2941 agcgcgggct cacgcgtgct gcgagcgggc gacgcatgag ctctgcgctc cagctcgacg
     3001 aagagaaggt gcggcggtgg ctcgggctcc tgcgccaccc gaaccggctc gactcgccgg
     3061 aggtgcgcgc gctcctgcgt gcgcatgggc gcctcccggc cgacgcgacg ccgctggacg
     3121 tgggccgcgc tgcggcgcgc tttctgactg acacgatcga ccggctcgcc gcgccgccgg
     3181 acgcctcggc cggcgaacgt ctcccgcatg cggtgctgcg cacgtgcttc gtcgacgggg
     3241 cgaagctgta ccaggccgcg gcgaagctcg ggctgtccga gcgtcagctc acgcgcgagc
     3301 gcgggcgcgc gatatcgctg ctgacggcgc agctgtcgct cggccccctc cgctctcggt
     3361 tcgtccccga ggagatcccg acgatcgacg acttcgtccc gcgcgcggac ctcgtcgcgg
     3421 cgatcaccga gacgctcggc gagggccgca gcgcccgcgt gcacgggcct cccgggatcg
     3481 gcaagaccag cctcgtcgcg gaggtcgcga tcgcggagag ccgccggagc gcggtgtggt
     3541 ggtatcggtt ccggcgcggc gtcaacgaca cgctccacgc ggtgctgttc gagctcgggc
     3601 agtggctggg gagccagggg ctgaccgacc tcgcgacgta cgtgacgccg aacgtggccg
     3661 ccctcgacag cgcggtcgcg tcgaggatcg cgctgcgcgc cctcgccgac acgaacgccc
     3721 tcgtcgtctt ggacgactac cagacggtcg aggacgacct cccgatcagc gggttcttcg
     3781 acgaggctct ggtgcgcacg ccgacgctgc ggctgatcac ttcgagccgc caccggccgg
     3841 ggaccgcgca cgcgcgggcc gcgatcgagg tcccgccgtt ccgcctcgaa cagaccgaac
     3901 ggctgctgct gcacgcgggg acgcggcggc cgcgcgagac gatcgccaag atccacgcgt
     3961 ggaccggcgg caacccccac ctcgtcaagc tctcggcgtc gtggctcaag agcgcgactc
     4021 cggcggagat cgcacggggc gtcgaggcgc tgatcgaccg cgaggaggtc caggccttcc
     4081 tgctcagcaa catcaccgag ctgctcgact ccgacgaccg cgggatcctc gaagccgcga
     4141 gcatcttccg gggcaggttc tccgacgacg cactggcata cgtcgcccgc cagacgcgcg
     4201 gcgcggtgca ggacgcgagc caccgcctgg tccgctccta cgtcgccacg cgcagccgtc
     4261 agggcgtctc cgcgttcttc cacggcagcg tccgcgacta cgtgtacgag aggctggatg
     4321 ccgaggtgcg ctccgggctc cacctgcgcg ccgcggcctg gttccaccgc ctcggcgacg
     4381 acgaggagac cgcgtaccac cgcgatcgcg gtgggctcga ggggggctag agccgcccta
     4441 ggaaggccag cacgcgttcc ccgacgaggg cggcggcatc ctggtcgtag tcgggccggc
     4501 tcgaatccgt gaacaggtgc ttgctccccg ggtagaggaa cagatcggcg tgcgggacct
     4561 tcccggcgat ggcgcgcgcc acgtccgcgt cgccccagtc gtcggcctcc atcgtgtgca
     4621 tctggagcgg cacgccgtcc ggccacgacc cgccgaactc ttcgagcggc accgccgcgt
     4681 ggaggaacac cgcgccgcgc gcgccgggcc gcgtctgcgc gagcatctgc gccggcatca
     4741 cgcccaacga catccccgcg tagacgaggc cgggcgagaa ctcctccgcg gcctccgtgc
     4801 cccgcctgag gcgggcgtca ggtgaggagg ctcggccgcc tccccacgag ctcgagcaac
     4861 aacgaggacg cgatccggta ctgctccagg gcctccgagg tcttgccctc gcgcaccagc
     4921 aggtcgccga gccccgcgtg cgcctctgcg cgctcggcga gcatcgagtc gtcgtcgccc
     4981 gcgagctcga ggcagcgctg caggtgcgtc gcggcggaag cggggtcgcc gtcgatgtcg
     5041 atgcgcgcca gctcgcggtg gagcacgacc agcacgcgcg ggtgcagctc gcggtcgcgt
     5101 tcgaggttcc cgagcgttct cgcgaggagc tcacggctct ccccgtgcag cccttcggcc
     5161 cgcaggtcct ccgccatctg cagcgcccac gacgcgccct tctcgctcca cagctcctcc
     5221 acggcgacgc cgaggcgctg cgcgaagtag tcgacggcgg cacgcgacgg ctcgcggcgc
     5281 cccgcttcga ggacgctcac gtacgcgtgg ctgaagcgcc tcccggcgag ctcggcctgc
     5341 gtcatccctg cggcgagccg gagctcgcgc aagcgccggc ccacccgtac gagctcgtcc
     5401 tgggacatcc ggcgacggcg cggcatgacc cctcagacga ccgcgaggct cgcgcgccgg
     5461 cgcatcccct gcaacgcgac cgtcaggtcc tcggaggcga tgcggacgtt cgcggcgccg
     5521 gtcccctcgc gcgacgcccg cgtcagagtc ttcatgccgg cgaggtcacg aagcctcttc
     5581 aggtcggcgc cggacatccc ctcggtcgcg agcgccagct cgcgcaggtc gacgtcgggg
     5641 gcgaccgaag ccgacgcgag gagccggcgg agcagctgca gcctgcccgc ggcgtcggga
     5701 aggccgatct cgaccggcgc gagccgtcgc tctcgaaccg ccgggtcgac cacgtcggga
     5761 cggttcgtcg caccgacgag gagcacaccg cccccggtag cgagcccgtc cagctccctc
     5821 aggaactgcg acaccacgcg ctcctcccac cggttggcgg agtcgccgcc gcgtgcccgg
     5881 agcagcccgt cgatctcgtc gacgaagatg atcgaaggcc tgttcgccct cgccttcgcg
     5941 aacagcttcg cgacgcgctg ctccgactcg cccgcccact tcgagagcag gtccgcggcc
     6001 gaatgctcgt agaagctcgc gtcgatctcg ctcgccatcg cccgcgcgat cgtcgtcttt
     6061 cctgtgcccg gagggccgtg caggaggatc cccggagggg gctcgatccc cagcgaccgc
     6121 gcgagatctg gcttcacgaa gacgttcacg aggtccatca gctgctcgcg cgtgtcgtcg
     6181 ggcagcacga cgtcgtccca tgtgacgcgc tcttcgagga cggcgcggtc cctccccgag
     6241 ccgtgccgca cggcggtgag gaggtcggac ccggagatgg gccggctcga cgcgagcgcg
     6301 gcctgagccg cgagcgtcac gatctgctcg agcgctgccg cgttgcgtcc gcccgtcgcg
     6361 tcgaccaccg cgtcgacgtc gacggcgtcc cagtccaccg agtccgtgcg cttcttcaga
     6421 tggagcagga cgatctcgcg ccgcgcctcg cggtccggga gtggcacccg tatgcgcgcg
     6481 tcgaacctcc cctctcgcaa tcccgggtcg agccggtcga cgtcgttcgt cgccgcgcag
     6541 acgaggaggc cgggcgtcga ccggcacgtc tcgagcgacg tcatcagctg cgtcacgacc
     6601 tcacggtgct ccgaaccggg cgcttcgtcc cgcgccccgg cgagggagtc gacctcgtcg
     6661 aggaacagca ggcacggcgc cgacgcgatc gcgaggtcga acacccgctg gacgttcgcc
     6721 gcggtctcgt gagcgagagg gctgccgatc gtcgccggcg taacgcggag atacctgagg
     6781 ccgtactccc cggcgagggc gcggctcagc aacgtcttgc cggtcccggg agggccatag
     6841 aacaggatgc cgttgtgcac gagccggtag cgcgccgcct cgtcggggtt ctcgaggatg
     6901 gtcccgacgg tcgcgcgcag ctgctccttg acctcgtgca ggccggcgac gtcgtcgaac
     6961 gtctcgagct cgtgaggggg cacgatcgca ggaccgctcg atcccgcgct cctcgccggg
     7021 gcctgcttcg gctgcgcctg cacggcgagg acctccgccg cgagccggag ccccgccgct
     7081 tcgacgaggt gaaccgtggc cgccgcttcc atcgacgccg ccggccctgc gagacgcgcc
     7141 tgctcgaggg ccgccttcgc ccgttgccgt tccgagatgt tgtcgaggag cgcgtcccgt
     7201 tgcgcgcggc cgatggtcgc gcggccgagc gcctcgttca acgcgacgac gagcggaccg
     7261 ccgacgagcc acagctcgcg ccccgcgcgg tcggcggccg ctcccgctgc gccgaagtcc
     7321 cgtcgttcga gcgcggcgag cgcttccgag aggtccgtgg acaccgcggc gacccgctgg
     7381 atctcgggct cgaccttggc cctcagctcg gcggagtcga gggccgcgag ccggcgcagc
     7441 gcggagtcga attcggtcgc ggcgtcccgg agcctcgcga gagccggctc ggccgccgcg
     7501 gccgcgacgg tctcctgctc cgcccagtcg cgctcgtgct cggcacgtcc cgccctccgc
     7561 ttccaccacg gcagcgcggc gatcgcctcg cccttctcga tctcgtgcag gacgcgccgg
     7621 tccgtgacgt gacgagcgtt gcgctggtag agcgcgacct ccgatcgccg gggaccggtc
     7681 acgtgcgccc ctccacggcc acgaccccgc cgaacagcgt gacctcgggg cggacgagcg
     7741 ccaggatcga ctcgaggtcg ggaccctcga tcgtctcctc ggcctcgagc ctctcggcga
     7801 gggcgcccag gacgtcgcgg tgccgcgtga gcagggccgc ggcctcctcc tcggcctccg
     7861 cgatcagccg gtcgacctcg gcctccatct cctgatgcgt gagggcggag accgggccca
     7921 gggacgcggt ctcacccagc agctcgtcct gcgcgcgtgc gagcagccgg cgtctgcgct
     7981 cccccatgcc gaagcgcccg acgacgtcca tcgcctggtc ggtcgcgaac tggaggtcgt
     8041 cctctgcccc cgtcgacggc tcgccgaaca gcagcatctc ggccgcgatc ccgccgagcg
     8101 cgatcacgat ccctccgacc agctccgagc gcgtctgcaa cgcggagtcg tcaccctgca
     8161 gcgcgatcgt gccgagtgac ctgccgcgcg ccaggatcga gactcggtgg acctgctcga
     8221 gccgtccgga cgcggcggcg acgatcgcgt gacccgcctc gtggaacgcg gccctcttgc
     8281 gctcctccgg cgagagcacg cggcccttct tcttcggtcc ggcgagcacg cgctgcacgg
     8341 cctcttcgag ctcggccatc tcgatctcgg gcttgccctc gcgcacggtc agcagcgcgg
     8401 cttcgttgag cacgttcgcg aggtcggcgc cggagaagcc cggggtgcgg cgcgctatca
     8461 ccgtgaggtc ggccagaggc gacaccggct tgccggcggc gtggagctcg aggatctcga
     8521 gacgccccgc cgggtccggc tgctcgaccg tcacgtgccg gtcgaaccga ccgggccgca
     8581 gcagcgcggg gtcgaggatg tccgggcggt tcgtcgcgcc tatgacgacg atccccgcgc
     8641 tcagctcgaa cccgtccatc tcgacgagga gctggttcaa ggtctgctcg cgctcgtccg
     8701 cgccgccgct gccgccgccc gcagcgcgct tgcggcccgc ggcgtcgagc tcgtcgatga
     8761 agacgatcgc cggggcgacg gcgcgcacgc gctggaacag gtcgcggacg cgggcagcgc
     8821 cgactccgac gagcgactcg acgaactcgg ctccggcgac cgagaagaac ggcactccgg
     8881 cctcaccggc gacagccttc gcgagcaggg tcttgccgca gcccggcggg ccgaagagca
     8941 gcacgccctt cggcgggagc gcgccgacct cttcgtagcg agcggggtcc ttcaggtagt
     9001 cgacgacctc gcgcagctcg gctaccgctt cgtccgcgcc ggcgacctgg tcgaacgtca
     9061 cgggggccgc gccgcctcgc ctccggcgcc ggtcgccgat ggtcccgaac gtcatgacct
     9121 cgccgatccc ggccgagccg ccgcgcgacg ccgagaacag cagcgcgaac aggttcgcga
     9181 ggatcatcag cggcagcagg aacgtcgaaa ccgtcccgat cacgtccttc gacggctgcg
     9241 ggtcgatcga cacccgggcc cccgcctccg tcgtcgtctc gactaggaac gcggtcaccg
     9301 cgtcgctgct gggatacgcg aggaagaact cgcccgagcc gcccggctcc cccgcgccgc
     9361 cggagtacct cccggtgatc gtcgcgtcgg cgtcgaggaa ccgtgcctcc tcgagacggc
     9421 cctcggatgc gagggagctc agctcgtcga gcgtgagctc atgcccgtgc gccggtgggc
     9481 gcagatacgc gagcgccgcc gcgaacaggc ccaggagagc gacgagcgac gccgtgagga
     9541 cgacgagcaa gcgcctgctc cccgtcttct tgcggccggc gcgggcgcgg cgacgtcgga
     9601 gcaaccagct cgacagcgcc ctccgggcgc gcggcgctaa cgatcctccg aacggcatgt
     9661 atcgcagtct cgaagcgaag ggcgggccgg gcgtcgccaa cgactcgcca gggcgtcgcc
     9721 aattaccggc ggacgtgcgc gctccgtgcg gt
//
LOCUS       DASQDO010000543         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_852400, whole genome shotgun sequence.
ACCESSION   DASQDO010000543 DASQDO010000000
VERSION     DASQDO010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852400"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VEV43_14940"
     CDS             complement(<1..1180)
                     /locus_tag="VEV43_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP24862.1"
                     /translation="MKKLVVLLCLSASVAAFTHPAAAQGHTVQHLTVRSKSADPVDVA
                     ISVFKPAGASAAAPVPVILESHGWGGRRRTSVGDGVVKPLLDAGFGVVSFDQRGHGGT
                     GGLRHLQDPAREAQDVISVIDKIATLDWVELDAPGDPVLGAIGGSYGGGFQTMTALTE
                     ISETGRTRFNALAPEITWFDFPTALAPEGVARTAWVSLVYAAGKAPCSTKTALSSAPA
                     DNLPKDPAECENKLAPWADNALAWALATGQWPAREDAVHAAVEEAAGIADLTSILRSH
                     SPRGFVEDGLALDIPIIQRQGVTDNLLNLNEGLDIFHRALTDDARSKSIFTAYNGGHP
                     LILHEGTPPVGSILPAASVPSGDPCNGGNSGWARLRIAFFEEVFAGNNPRVTLGARAY
                     N"
     gene            complement(1263..2123)
                     /locus_tag="VEV43_14945"
     CDS             complement(1263..2123)
                     /locus_tag="VEV43_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP24863.1"
                     /translation="MSTAWVVARHTFTECTRRKIFVIVPIVTAGFLALFALGAHYAFQ
                     SVSGTVRTGPAQLVDETSLAGSTLVGLSMFVTLFLGASLGIFLTFGVVRGDAETGVLQ
                     QLVVRPVARSGLLAGRFLGASFLCSTYVAVLYLACVTVVGLIGEWWPTSVVLPGLSLV
                     SGVIVVIALSLLGSVFLSALPNGIVVFMLYGAGLFAGLLVQLGEGLRSPSVETTGRIV
                     SWALPFEALYQSGLNSLTSSATGLTRVVVQLGPLGGAQEAGPGLVLWTLVYLAGVGAL
                     ALFVFNRKDL"
     gene            complement(2120..2962)
                     /locus_tag="VEV43_14950"
     CDS             complement(2120..2962)
                     /locus_tag="VEV43_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP24864.1"
                     /translation="MAPVLEMRQLVKRYGTTVALAGVDLEVSEGELVGLLGPNGAGKS
                     TLVKIGCGLVRPTSGSAHVCGAPAGSRQARAALGYLAELFRFPGWQRAGEVLELHQRL
                     TGSSGGAAERADLLELVGLGRVGSRRVDAMSKGMQQRLGIAQALVGSPRLLMLDEPTS
                     ALDPAGRRAVRRILEEVRARGITVLLNSHLLSEVELVCDRVFILLGGEVIAEGTPDDL
                     AASRGVEIETDQGTKKWPSARREDIPGLVRELVGAGERVFAVRTAKPTLEDVYMEAVG
                     GEMG"
     gene            3171..3452
                     /locus_tag="VEV43_14955"
     CDS             3171..3452
                     /locus_tag="VEV43_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24865.1"
                     /translation="MRESTGRHTGSKNLTRITEEVLREHGFEPFHDEDGNVRLRNCPF
                     HLLSQEYTQLVCGMNLDVMRTMVEELGLERLEARLEPQPGTCCVALRRV"
     gene            3457..3585
                     /locus_tag="VEV43_14960"
     CDS             3457..3585
                     /locus_tag="VEV43_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24866.1"
                     /translation="MLQGGQSASLRRAIDPVRAERAAADLLAALGVDLSAREPRVP"
     gene            3802..>4379
                     /locus_tag="VEV43_14965"
     CDS             3802..>4379
                     /locus_tag="VEV43_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24867.1"
                     /translation="MRNLASRIRRAAPWTWFAAANAVAITAYFLLPGDVLPKAVFVAI
                     GLASGITILRRARTTSGPRVWVAFGATQIAWATGDAIWAAYEITGGPAPYPSFADVLY
                     LAGYPLMMYGVVAHTLRQVGDDARKVLVDAGMIALVLAIGGWELVVRPYVAQAHLTPG
                     EDFVGFLYPAMDLALASVSLILLHGSGARSAS"
BASE COUNT          684 a         1542 c         1542 g          611 t
ORIGIN      
        1 ggttgtaggc ccgcgcgccc agcgtcaccc gcgggttgtt gcctgcgaac acctcctcga
       61 agaacgcgat ccggagccgc gcccagccgg agttgccgcc gttgcaggga tcgccgctcg
      121 ggacgctggc ggccggcagg atcgacccga ccggcggcgt cccctcgtgc aggatcaggg
      181 ggtgaccgcc gttgtacgcg gtgaagatgc tcttcgaacg tgcgtcgtcg gtcagcgcgc
      241 ggtggaagat gtcgagccct tcgttgaggt tcagaaggtt gtccgtcacg ccctgccgct
      301 ggatgatcgg gatgtcgagc gccaacccgt cttcgacgaa gccacgcggg ctgtgcgacc
      361 ggaggatgct cgtcaggtcg gcgatccccg cggcctcctc gacggctgcg tgcacggcgt
      421 cctcccgagc cggccactgc cccgtcgcga gcgcccaggc gagcgcgttg tcggcccacg
      481 gggccagctt gttctcgcac tccgccgggt ctttcggaag gttgtccgcc ggcgcggacg
      541 agagcgccgt cttcgttgag caaggagcct tgcccgcggc gtagacgagg ctgacccagg
      601 ccgtccgcgc gactccctcc ggtgcgagcg cggtcgggaa gtcgaaccac gtgatctccg
      661 gcgcgagggc gttgaaccgc gtccggccgg tctccgagat ctcggtcagg gccgtcatcg
      721 tctggaagcc gccgccgtag gagccgccga tcgcgccgag gacgggatcc ccgggcgcgt
      781 ccagctcgac ccagtcgagc gttgcgatct tgtcgatcac cgagatgacg tcctgcgcct
      841 cgcgggcggg gtcctgtagg tgacggagcc cgccggtccc accgtgtccg cgctggtcga
      901 agctgacgac gccgaagccc gcgtcgagaa gcggcttgac gacaccgtcg cccaccgacg
      961 tccggcgacg tccgccccag ccgtgcgact cgaggatgac ggggacggga gccgcggccg
     1021 acgctcccgc gggcttgaac acgctgatcg ccacgtcgac cgggtcggcc gacttgctcc
     1081 gcacggtgag gtgctgcacc gtgtgaccct gcgcggccgc aggatgcgtg aaggcggcga
     1141 cggatgcgga caggcagagc aggaccacga gcttcttcaa gttccgacct cctggacgag
     1201 cttggttcgc ttcggcggtc caggggggtt ctccggggag gggccggctc ctgcgggcgg
     1261 cttcagaggt ccttgcggtt gaacacgaac agggccaggg cccccactcc cgcgagatat
     1321 acgagcgtcc acaggacgag gcccggcccc gcttcctgcg ccccgccgag ggggcccagc
     1381 tggactacga cccgcgtgag cccggtggcg ctcgacgtca gcgagttgag accggactgg
     1441 tagagggcct cgaacggaag cgcccacgag acgatgcggc ccgtggtctc gaccgaaggt
     1501 gagcgcaggc cctcgcccag ctgcacgaga agccccgcga acagcccggc cccgtagagc
     1561 atgaagacca cgatgccgtt cgggagagcc gacaggaaca ccgatcccaa cagcgacaga
     1621 gcgatgacga cgatcactcc cgagacgagc gacagaccgg ggagcacgac cgacgtcggc
     1681 caccactccc ctatgagccc gacgacggtc acgcacgcga ggtagagcac ggccacgtac
     1741 gtcgagcaga ggaacgacgc gccgaggaac cgacccgcga ggaggccgga gcgcgccacg
     1801 gggcgcacga cgagctgctg gagcacaccc gtctcggcgt cgccccgcac gacgccgaac
     1861 gtcaggaaga tgccgagcga cgcaccgagg aacagcgtga cgaacatcga gagcccgacg
     1921 agcgtcgacc cggcgagaga cgtctcgtcc accagttgcg ccgggcccgt ccggaccgtc
     1981 ccgctcacgc tctggaacgc gtagtgcgct cccaaggcga acagcgcgag gaagcccgcg
     2041 gtgacgatgg ggacgatcac gaagatcttg cggcgcgtgc actccgtgaa cgtgtgacgg
     2101 gcaaccaccc atgcggtgct caacccatct cacccccgac ggcctccatg tagacgtctt
     2161 cgagcgtcgg cttggccgtg cgcaccgcga acacgcgctc gcctgcgccc acgagctcgc
     2221 gcacgagtcc ggggatgtcc tcccgacgcg ccgacggcca cttcttcgtg ccttggtcgg
     2281 tctcgatctc gacgccccgg gacgcggcga ggtcgtcggg cgtcccctct gcgatcacct
     2341 cgccgccgag gaggatgaag acccggtcgc agacgagctc gacctcgctg aggaggtgcg
     2401 agttcagcaa gaccgtgatg ccgcgggccc gcacctcttc gaggatgcgg cgtactgccc
     2461 ggcggcccgc ggggtcgagc gcgctcgtcg gctcgtcgag catgaggagc cgcggcgaac
     2521 cgaccagcgc ctgcgcgatc ccgagacgct gctgcatgcc cttggacatc gcgtcgacgc
     2581 ggcggctgcc gacgcgacca agtcccacga gctcgaggag gtcggccctc tcggcggctc
     2641 cgccggacga tcccgtcagc cgctggtgga gctcgagcac ctcccctgcc ctctgccagc
     2701 cggggaagcg gaagagctcc gcgagatagc cgagcgcggc ccgcgcctgc cgcgagcccg
     2761 cgggggcgcc acagacgtgc gcgctgcccg aggtgggccg gacgaggccg cacccgatct
     2821 tgacgagcgt ggacttgccg gcgccgttgg ggccgagcag gccgacgagc tcgccctcgc
     2881 tgacctcgag atcgacgccc gcgagcgcga cggtggttcc gtagcgcttg acgagctggc
     2941 gcatctcgag cacgggagcc atggcgccga ccctaccgac gagatgtcag ggagacgcgc
     3001 gggagctgac ctcttttctt gcgggggcga gcccgcgagg tcagagtctc gctcccccac
     3061 cgcgaatacg agctcgccgc ccgggtcttc gctcgggcgc tcacgacgga gggcggcccg
     3121 gctcccgtcc gcgacgcgac cgcgccctcg gcacggagac ggcggacgag gtgcgcgaga
     3181 gcacgggccg ccacacaggg tccaagaacc tgacgcgcat caccgaggag gtcttgcggg
     3241 aacacggctt cgagccgttc cacgacgaag acggcaacgt ccggctccgc aactgtccgt
     3301 tccacctgct ctcccaggag tacacgcagc tcgtgtgcgg gatgaacctg gacgtcatgc
     3361 ggacgatggt cgaagagctc gggctcgaaa ggctcgaagc ccggctcgag ccccagccgg
     3421 gcacgtgctg cgtcgccttg cgccgggtct gagggtgtgc tgcagggcgg gcagagcgct
     3481 tccctccgcc gggcgatcga ccccgtgcgg gccgagcggg ccgccgcgga tctcctcgcc
     3541 gcgctgggcg tcgacctctc cgcgcgcgaa ccccgcgtcc cgtgacgagt tccttgcgcc
     3601 gtgcggggag aaagcgtgag cgccaccgaa ccgacggcac tcgagcaagg gggcacgtga
     3661 tggcccggcg cgggaagttc atgccgcttc gggactagtc atctcgcgga aaccgaaacg
     3721 gacttttcgg gcgccgtgag gatcgacatt tcattcgccc cggctatggg atcgcgcgcc
     3781 gctcgtgacg atgatctgga catgaggaat cttgcgagcc ggatccgccg agcggccccc
     3841 tggacgtggt tcgcggcggc gaacgccgtt gcgatcaccg cctacttcct gctgccggga
     3901 gacgtgctcc cgaaggccgt gttcgtcgcg atcggcctcg cgagcgggat cacgatctta
     3961 cgccgcgcgc ggacgaccag cggcccccgg gtctgggtcg cgttcggagc gacacagata
     4021 gcgtgggcga cgggagacgc gatctgggcg gcttacgaga tcaccggggg gccggccccc
     4081 tatccctcct tcgcggacgt gctctacctc gcggggtacc cgctcatgat gtacggcgtc
     4141 gtcgcgcaca ctctccggca ggtcggcgac gacgcacgga aggtgctcgt cgacgcgggg
     4201 atgatcgcgc tcgtgctcgc gatcggcgga tgggagctcg tcgtccgccc ttacgtcgcg
     4261 caggcgcacc tcacgccggg cgaggacttc gtcggcttcc tataccccgc catggacctg
     4321 gcgctcgcgt ccgtctcctt gatcctgctg cacggcagcg gcgcccgcag cgcgtcgaa
//
LOCUS       DASQDO010000544         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1036077, whole genome shotgun sequence.
ACCESSION   DASQDO010000544 DASQDO010000000
VERSION     DASQDO010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036077"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..531)
                     /gene="rpsO"
                     /locus_tag="VEV43_14970"
     CDS             complement(262..531)
                     /gene="rpsO"
                     /locus_tag="VEV43_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006546780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HYP24868.1"
                     /translation="MALLKEQKQEIISDNARGDVDTGSPEVQIALLTQRITELTEHLK
                     THKHDHHSRRGLLRMVGRRKRLLAYLQRQDVERYRALIAKLGLRR"
     gene            complement(615..1559)
                     /locus_tag="VEV43_14975"
     CDS             complement(615..1559)
                     /locus_tag="VEV43_14975"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HYP24869.1"
                     /translation="MTDVVVGIDALSAPPNGSAITIGTFDGVHLGHRALIALTIAEAR
                     ARDLTPVVLTWDRHPAATLRPDKAPPMLSSPERKIELLRETGADLVAVLPFDVPLSQR
                     EPESFVGDVLVKGLGARLVLVGEGWRFGRGRAGTVELLEELGRDLGFEARGVTLEEVG
                     GEAASSSRVRKAVTSGDLELAERLLGRPFDLDGVVEHGDARGAGLGFPTANLAIDPTI
                     ASPPVGVYAGRARSGDSWFPAATNVGVNPTFGGTGTPRIETYLVGFEGDLYGRALRVE
                     FWERLRDEKRFDSVDELIEQMGRDVEATKALVAERDPG"
     gene            complement(1556..>2092)
                     /gene="truB"
                     /locus_tag="VEV43_14980"
     CDS             complement(1556..>2092)
                     /gene="truB"
                     /locus_tag="VEV43_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYP24870.1"
                     /translation="LTGEIEQVPPMVSAVKIGGEKLYEKARRGEEVERAARRVTIYEL
                     TLLKHDPEAATATLEVLCSGGTYVRTLIHDLGAELGCGAHMTTLRRTAAAGFTEMDAI
                     ALDAVDESALRPLGDAVRELPRLDVDAATATAVSHGKPLEVEPPAPGPVALFHDDELI
                     AVYVAGERGFRPDRVVPS"
BASE COUNT          299 a          728 c          733 g          332 t
ORIGIN      
        1 cgatgcggaa cacctcgtcg ccgatcttgc gctcgacgta gatgggctcc ggggacatac
       61 cagtagtacc tgtagacatg cgtctcctcc tgaggagttg ggaggagagg cgccagcctg
      121 gccgattctc agtagggggc gcacgagcgg aaggtctcgt ggatcctcaa ctgataatcg
      181 accgaggggg ccggccctct ccgattacct gcagcgggtt ggggctccgg cgtcctcctt
      241 aatgtccgtg gggcgctgca actagcggcg caggcccagc ttggcgatga gcgcccggta
      301 gcgctcgacg tcctggcgtt gcaggtacgc gagcagacgc ttgcggcggc cgaccatgcg
      361 gagcaggccg cgccgggaat ggtggtcgtg cttgtgggtc ttgaggtgct cggtgagctc
      421 ggtgatgcgc tgggtcagca gggcgatctg gacctccggc gagcccgtgt cgacgtcccc
      481 acgagcgttg tccgagatga tctcctgctt ctgttccttg agcaaagcca tgcgcgtcta
      541 ccgaggtccc ttcgtgtcga tgatttgccc gcggccaaac cttccagccc tgagggccgg
      601 gccatcgtag caggtcaccc cgggtcccgc tcggctacga gggccttcgt ggcctcgacg
      661 tcgcggccca tctgctcgat cagctcgtcg accgagtcga accgcttctc gtcccgcagc
      721 cgctcccaga actcgacccg gagggcccgg ccgtagaggt cgccctcgaa tccgactagg
      781 taggtctcga tcctcggggt ccccgttccg ccgaaggtcg ggttgacgcc gacgttcgtg
      841 gccgccggga accaggagtc gccggatctc gccctccccg cgtacacgcc taccgggggc
      901 gaggcgatcg tcgggtcgat agcgaggttc gcggtcggga agccgagccc agcccccctt
      961 gcgtcgccgt gttcgactac gccgtcgagg tcgaaggggc ggccgagcag cctctcggcc
     1021 agctcgaggt cgcccgacgt cacggccttg cgcacgcggc tgctggatgc cgcctcgccg
     1081 ccgacctcct cgagcgtcac gccgcgggcc tcgaagccga ggtcgcgccc cagctcctcg
     1141 agcagctcga ccgtgccggc gcggccgcgc ccgaagcgcc acccttcgcc gaccaggacg
     1201 agccgcgcgc cgagaccctt cacgaggacg tcgcccacga acgactcggg ctccctctgc
     1261 gagagcggca cgtcgaacgg gagcaccgcg accagatccg cccccgtctc cctcaagagc
     1321 tcgatcttgc gctcggggct cgacagcatc ggcggagctt tgtcggggcg cagcgtcgcg
     1381 gccgggtggc ggtcccacgt aaggacgacg ggcgtgaggt cgcgggcccg ggcttcggcg
     1441 atcgtgagag cgatcagcgc gcggtggccg agatggacgc cgtcgaacgt gccgatcgtg
     1501 atggcggatc cgttcggggg cgcgctgagt gcgtcgatcc cgaccacgac gtccgtcatg
     1561 aggggacgac ccggtcggga cggaagccgc gttcgccggc gacgtagacg gcgatcaatt
     1621 cgtcgtcgtg gaaaagcgcg acggggccgg gggcgggcgg ttcgacctcc agcggcttgc
     1681 cgtgcgagac cgcggtggcc gtggccgcgt ctacgtccag ccggggcagc tcgcgcacgg
     1741 cgtcgccgag aggacgcaac gcggactcgt ccaccgcgtc gagggcgatc gcatccatct
     1801 ccgtgaaacc cgcagcagcc gtgcggcgca gggtcgtcat gtgcgcgccg cagccgagct
     1861 ccgcaccgag gtcgtggatc agcgtccgca cgtaagtccc gccggagcac aggacctcca
     1921 gcgtcgccgt ggccgcttcg gggtcgtgct tcaacaacgt cagctcgtag atcgtgacgc
     1981 gccgggccgc ccgctcgacc tcctcgccgc ggcgggcctt ctcgtagagc ttctcgcccc
     2041 cgatcttcac ggccgagacc atcggcggga cctgctcgat ctcgccggtc ag
//
LOCUS       DASQDO010000545        10979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_545950, whole genome shotgun sequence.
ACCESSION   DASQDO010000545 DASQDO010000000
VERSION     DASQDO010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545950"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="purH"
                     /locus_tag="VEV43_14985"
     CDS             complement(<1..1083)
                     /gene="purH"
                     /locus_tag="VEV43_14985"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYP24871.1"
                     /translation="MEVRRALISVSDKSGVVELARALTQRGIAIVSSGGTARALADAG
                     IEVTPVPDVTGAPEILDGRVKTLHPKIHGGILADRRKPAHVEQMDEQGIEPIDLVVCN
                     LYPFERTVAHPAVTEEDAIEQIDVGGPAMVRAAAKNFHSVAVVVNPERYDSVLAELSE
                     SGSISLETRRRLALEAFQHTGAYDASIAAYLAGIEHDLFPQRMLLAGTKVMDLRYGEN
                     SHQSAAFYANRGTPTGLAAAEQLHGKELSYNNLLDTDAAWKLAQDLEGAGAAIIKHSN
                     PCGVAIAGELAEAFRLAFECDTTSAFGGIVALTKTCDRATAEKIAEIFTEVVVAPDYE
                     DGALEVLMTKKNLRILKAGRYSLPEMD"
     gene            complement(1108..1695)
                     /gene="purN"
                     /locus_tag="VEV43_14990"
     CDS             complement(1108..1695)
                     /gene="purN"
                     /locus_tag="VEV43_14990"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYP24872.1"
                     /translation="MASRIVVLISGAGSNMLALVRACRAGEVPAEVVAVVADRPCDGL
                     ELARAEGIDTAVLDPRGYPSREHWSTALLDLVRTFSPDLVVSAGFMRILSPVFVHAYS
                     GRLINLHPSLLPAYPGAHAVRDALEDGASVTGSTVHFVDDEVDHGPVILQREVSIEPG
                     DDADTLHERIKGVEHGMLAEACRMVLDGRAALEGP"
     gene            1727..2746
                     /locus_tag="VEV43_14995"
     CDS             1727..2746
                     /locus_tag="VEV43_14995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYP24873.1"
                     /translation="MIWREPVRGGYPEWELFGLSGLELMQTFLKREGPRPPISHLFGM
                     LPTVMEPGVSVFEMPASPWLLSPPGFIQGGALAVHADGPLGCAVHTALPPATPYTTSE
                     LSMTFIRPVTQESGTLTAEGRVIHAGKSMALSEVSIADAAGRLVAHGTSRCFVFDPLS
                     PAPEPPDSLPPYDAPEYGTPDPYLRPVEGETLPPEIWKERSGLDVLQAGIRGELPAPP
                     IHYLTGLRPIDAGDGTATFVMPASEWLNSPLARVQGGTISLLADSALSCAVQTIVPPG
                     TTYLTLDLKVNFLRPVTADGSDLTAHGKVVHRGRTLAIANADVMNAEGKKVAFGTGTI
                     MLRGA"
     gene            complement(2762..4036)
                     /locus_tag="VEV43_15000"
     CDS             complement(2762..4036)
                     /locus_tag="VEV43_15000"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYP24874.1"
                     /translation="MRMRAIALAAVSAVTLSSSPAQGQQEYVYQPVCEVVTFSPRFAE
                     DRTAFCATAEYLPGTVQPSGNVLVRRSTDGGVTWEPLPSVGLQGLGLSWPVQILVSPS
                     YPDQTAIYVQIVYGPRPGLYRSTDEGETFAWVAPLATTGMLTRPLTAYTAAASIPPPL
                     GAGADAFTYASVEESRPAMLTPPLHRPVAGSPDGAAMFLLPPDFPSSSALVVARGVYE
                     PSPDPETWEWQVSIYRCDVTLTCGEKLAELPPGLDLVDADFLGDGTAYVVVERFGAGT
                     DTQVWRSTDGETFSRWESLDALAPRRDINGNSNVIVDVVDVPGTAGRFFARVSYQVEG
                     ESGAPRDRLFRSDDDGVTWRAVASSRRSRGRGYLPWFRRIGPIHAEGDLYMPSPDRLF
                     VLAYEVDARPGSEYMGPFCSVDGGRTWARACR"
     gene            complement(4158..4748)
                     /locus_tag="VEV43_15005"
     CDS             complement(4158..4748)
                     /locus_tag="VEV43_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24875.1"
                     /translation="MKKKLAGVVAAAVTLIGAAVAPSANAQTASAAVAIVFANVDGTS
                     VSCSLPTSTGVHLRSCSLTATAAGATVPTNFCEETASVSVLPGHASAGRLGCGVTVRA
                     LLTTEITTAEAEEGESYVHSCTGAGIGTATYSPAPTSAAVPISGPVAVTWIGGVVTIE
                     GALVNVGQASAGQISAEGFDLCGSELDPNPFAGRIN"
     gene            complement(4935..6062)
                     /locus_tag="VEV43_15010"
     CDS             complement(4935..6062)
                     /locus_tag="VEV43_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYP24876.1"
                     /translation="MDSQRHLFDIPAGVTYLNAAYLGPRLHSVDAAGQEALARTGQPW
                     TISPPDFFSGAESARERFAQLVGGDADGVAIIPAVSYGVATAAKNMPLARGQKVVVLE
                     EQFPSNVYEWRDAAARAGGGLVTVPRPADSDWTRAVIDRIDVYTGIVAVPSCHWTDGT
                     VVDLVRVGEAARAAGAALVVDATQSLGVRPLDVGAVRPDWLFAAGYKWMLGPFSLGYA
                     WVAPERRDGDPLERNWLTRAGAEDFAGLVRYTDEFALGARRYDMGERANFHLMPMGIA
                     ALDQLLSWGAEEIESYIEALTDEIADGATSLGWDVAPRQSRSRHMLGLRRAGGIPEKL
                     PNLLAEANVYVSVRGDSIRVSPHVYNDATDVARLLEALERA"
     gene            complement(6063..7121)
                     /locus_tag="VEV43_15015"
     CDS             complement(6063..7121)
                     /locus_tag="VEV43_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYP24877.1"
                     /translation="MTLREVEGLPGPPWERDIRGTFEKLTVESELLADNPLGDPARRP
                     LYVYLPPGVETSSDHAYPSVYVIQGMTGQLDMWLGRSAFEPTMIERVDAAFCADGAPR
                     AVVVFVDAWTSYGGSQFVNSAATGRYMDYLCDEVVPFVDSRYPTAASRDHRGITGKSS
                     GGYGAMMVPMLRPDVFGGLASHAGDALFEVCYQPEFRDTARILRDRFEGSYDVFWERV
                     RSAERFDFGLFGHPLDSYAMAACYSPDENEPGKVVLPFEIETGRLIPDVWERWLEWDP
                     VRVAPRRMEALASMKHVYLDAGRSDEYFLDLGAIAFSKELTAVGVEHELVLFDGSHGG
                     LQYRYPKAITDLARSLEA"
     gene            complement(7118..7999)
                     /gene="sucD"
                     /locus_tag="VEV43_15020"
     CDS             complement(7118..7999)
                     /gene="sucD"
                     /locus_tag="VEV43_15020"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HYP24878.1"
                     /translation="MAILIDDSTKLVVQGLTGREGSFHAGRNKQYGTNLVAGVTPGKG
                     GSDVDGVPVFDTVRDAVEQTGANTSMVFVPPRFAADAILESYDAGIDVIVAITEGIPA
                     MDMARTVPYLRRGDRDVALIGPNCPGLISPGKANVGIIPGEICMPGPVGLVSRSGTLT
                     YQIVHELTQRGIGQSTCVGIGGDPLPGSQFIDVLTRFEADPETELAVLVGEIGGDAEE
                     RAAAWVQANMSKPVVAYIAGFTAPPGKRMGHAGAIVSGSKGTAQAKAEALEAAGIRVG
                     KNPTEVAEIVAEIRGGS"
     gene            complement(7999..9150)
                     /gene="sucC"
                     /locus_tag="VEV43_15025"
     CDS             complement(7999..9150)
                     /gene="sucC"
                     /locus_tag="VEV43_15025"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HYP24879.1"
                     /translation="MDLFEYQGKELLRKFEVPVPEGRVATTPDEAEEAAKGLGGKVVV
                     KAQVQVGGRGKAGGIKLAGSPDEAREVASKILGMDIKGHTVKRVLVERAGDIKAEYYC
                     SFLLDRSNRNFLGMMSAKGGVDIEEVADTDPDAIAMVHIDPLLGMSDFHAREMVFGSG
                     LDAAARKDAISLLPKLYRAFVELDASLVEVNPLVLTGEGRVVALDAKVSLDDSAAYRH
                     PVFDQLKAAHEIPEQERIAKEKGLNFIKLDGDVGIIGNGAGLVMSTLDVVKAAGGEAA
                     NFLDVGGGAGAELLSSALEVITSNPDVRSVLINIFGGITRCDLVAEGIIDALGRLDVK
                     VPIVVRLDGTNAEQGRKMLADAPHPMLKTSDTMLGAAQLAVDLSHGGAN"
     gene            complement(9150..9569)
                     /locus_tag="VEV43_15030"
     CDS             complement(9150..9569)
                     /locus_tag="VEV43_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HYP24880.1"
                     /translation="MPSGRPRILVAKPGLDGHDRGIKVVARALRDAGMEVIYTGLHQT
                     PEQIASAAIQEDVDAVGLSCLSGAHMTLFPRVAQLLEEQGGEGMLVFGGGIIPDADIP
                     KLKESGISEIFTPGTTTKTIVEWVRDHVSAENEEAAG"
     gene            9659..>10979
                     /locus_tag="VEV43_15035"
     CDS             9659..>10979
                     /locus_tag="VEV43_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYP24881.1"
                     /translation="MPETAPEAIETLIDGGSFEPAGDAVPAGDPLGYPGYTDALARAR
                     ERSGTDESVVTGAATIGGIAVEVAAFEFPFMGGSMGAAAGELLARSMERAAARGVPFV
                     LRTATGGARMQEGMASLVQMPKVAGARIELGEARQPFVAVLGNPTTGGVLAGLAALAD
                     VTIAERGATVGFAGPRVAERATGRPLSKTSHTAEAALDAGLVDAVVDTSSVPAVVEWI
                     LSMLTSRGDDGEHTPPETTGDHLDAWEAFQRVRSPEWPQSVLLAAQMGGPAFELRGDR
                     AGSDDRGCVAFLAQVDGRGALILALQRGVAPGAADYRKAIRCVRIAGRVGLPIVTLVD
                     TGGADPSEGSERHGIAWAIAELFDAILSAPVPVVSVLMGEGGSGGALAFACGDALLAF
                     EETAFAVIAPELAAEILWRDAGRAEEAAKLLKMGAADLARLGICDEVL"
BASE COUNT         1668 a         3827 c         3785 g         1699 t
ORIGIN      
        1 gtccatctcc ggcaacgagt accggccggc cttcaggatg cggaggttct tcttcgtcat
       61 gaggacctcg agcgcaccgt cctcgtagtc cggggcgacg acgacctcgg tgaagatctc
      121 cgcgatcttc tcggccgtcg cgcggtcgca agtcttcgtg agcgcgacga tgcccccgaa
      181 cgcggacgtg gtgtcgcact cgaaggcgag ccggaaggcc tccgcgagct cgcccgcgat
      241 cgcgacgccg cacgggttcg agtgcttgat gatcgcggcc cccgcgccct cgaggtcctg
      301 cgcgagcttc cacgccgcgt cggtgtcgag caggttgttg taggagagct ccttgccgtg
      361 gagctgctcg gccgccgcga ggccggtggg ggtcccgcgg ttggcgtaga acgccgccga
      421 ctgatgcgag ttctcgccgt agcgcaggtc catcaccttc gtccccgcga gcagcatgcg
      481 ctgcgggaac aggtcgtgct cgatcccggc gagatacgcc gcgatcgatg cgtcgtaggc
      541 gccggtgtgc tggaaggctt cgagcgcgag gcggcggcgc gtctcgagcg atatggaccc
      601 gctctccgac agctccgcca ggaccgagtc atagcgctcg gggttcacga cgaccgcgac
      661 cgagtggaag ttcttcgccg cggcccggac catcgcggga ccgccgacgt cgatctgctc
      721 gatcgcgtcc tcctcggtca ccgccgggtg cgcaaccgtc cgttcgaacg gatagaggtt
      781 gcacacgacg aggtcgatcg gctcgatgcc ctgctcgtcc atctgctcga cgtgggcggg
      841 cttgcggcgg tcggcgagga tcccgccgtg gatcttcgga tggagggtct tcacacggcc
      901 gtcgaggatc tccggggccc ccgtcacgtc cggcaccggc gtcacctcga tcccggcgtc
      961 cgccagggcc cgagcggtcc cgccggacga gacgatggcg atgccgcgtt gcgtcagggc
     1021 gcgcgcgagc tcgaccactc ccgacttgtc ggagacgctg atgagcgccc ggcgcacctc
     1081 cacgccgggc atcctattgc gtcgtggtca aggcccttcg agggcggcgc ggccgtccag
     1141 gaccatgcgg catgcctcgg cgagcatccc gtgctccacg cctttgatgc gctcgtgaag
     1201 ggtgtctgcg tcgtcgccgg gctcgatcga gacctcgcgc tgaaggatca ccggcccgtg
     1261 gtccacctcg tcgtcgacga agtggacggt cgacccggtg accgatgcgc cgtcttcgag
     1321 cgcgtcccgg accgcgtgcg caccgggata cgcggggagc agcgacgggt ggaggttgat
     1381 cagacggccc gagtacgcat ggacgaagac cggcgagaga atcctcatga agcctgcgga
     1441 cacgacgagg tcgggcgaga acgtgcgcac gaggtcgagc agcgccgtgc tccagtgttc
     1501 ccgcgagggg tagccgcggg ggtcgaggac ggcggtgtcg atgccctcgg cgcgcgcgag
     1561 ctccagcccg tcgcagggac ggtcggcgac gacggcgacg acctcggccg ggacttcgcc
     1621 ggcccggcac gcgcgcacga gcgcgagcat gttcgacccg gcccccgaga tgaggacgac
     1681 gatgcgggaa gccatgggcc gagcctagcg gcgtaggctc gcgcccgtga tctggcggga
     1741 acccgtgcgc ggcggctatc cggagtggga gctgttcggc ctctcggggc tcgagctcat
     1801 gcagacgttc ctgaaacggg aagggccccg gccgccgatc agccatctgt tcggcatgct
     1861 cccgaccgtc atggagcccg gggtctcggt cttcgagatg cccgcgtcgc cgtggctcct
     1921 cagcccgccg gggttcatcc aaggcggcgc cctcgcagtg cacgcggacg gcccgctcgg
     1981 ctgcgccgtc cacaccgcgc tgccgcctgc caccccttac acgacgtcgg agctctcgat
     2041 gacgttcatc cgtccggtca cgcaggagag cgggactctg acggcggagg gccgcgtcat
     2101 ccacgcgggg aagtccatgg cgctttccga ggtgtcgatc gccgacgccg cggggcgcct
     2161 cgtcgcccac gggacgtcgc gctgcttcgt cttcgacccc ctgtcgcccg cgcccgagcc
     2221 gccggactcg ttgccgccgt acgacgcgcc cgagtacggc acgcccgacc cctatctgag
     2281 gccggtcgag ggcgagaccc tcccgccgga gatctggaag gagcggtcgg ggctcgacgt
     2341 gctccaggcg ggcatccgag gagagctccc cgcacctccg atccactacc tgaccgggct
     2401 gcggccgatc gacgccggtg acgggaccgc cacgttcgtg atgcctgcga gcgaatggct
     2461 gaacagtccc ctggcgcgcg tgcagggcgg gacgatctcg ctcctcgcgg actcggcgct
     2521 gtcgtgcgcg gtgcagacga tcgtcccgcc gggcacgacg tacctgacgc tcgacctgaa
     2581 ggtgaacttc ctgcgccccg tgacggcgga cgggtcggac ctgacggcgc acgggaaggt
     2641 agtgcaccgg ggccggacgc tagcgatagc gaacgcagac gtaatgaacg cggagggcaa
     2701 gaaggtggcg ttcgggaccg ggacgatcat gctccggggg gcctagacag cgttcagacg
     2761 cctagcggca cgcccgcgcc catgtccggc ctccgtcgac cgagcagaac ggacccatgt
     2821 actccgagcc aggtcgcgca tcgacctcgt acgcaagcac gaacaaccga tcgggcgacg
     2881 gcatgtagag gtctccctcg gcgtggatcg gcccgatacg ccggaaccac ggaaggtagc
     2941 ccctgccacg cgaccgtcga gatgacgcta ccgcccgcca cgtgaccccg tcatcgtcgc
     3001 ttcggaagag ccgatcgcgg ggggctccgg actcgccttc gacctggtag gacacgcggg
     3061 cgaagaaccg ccccgctgtt ccaggaacgt cgacgacgtc cacgattacg ttcgagttcc
     3121 cgttgatgtc gcgccgcggt gcgagagcgt cgaggctctc ccaacggctg aaggtctctc
     3181 cgtcggtcga ccgccacacc tgtgtgtccg tgcccgcccc gaatcgttcg acgacgacgt
     3241 atgccgtccc gtcgccgagg aagtcggcgt cgacgagatc cagccccggt gggagctcgg
     3301 cgagcttctc tccgcatgtg agcgtcacgt cacagcgata tatcgaaacc tgccattccc
     3361 acgtctcggg atccggtgac ggctcgtaga cgccgcgcgc taccacgagc gcgctcgacg
     3421 atgggaaatc cggaggaaga aggaacatcg cagccccgtc gggagagcct gcgacagggc
     3481 ggtgaagcgg cggcgtgagc atcgcgggcc gggactcctc gacgctcgcg tacgtaaacg
     3541 cgtcggcgcc cgctcccaac ggcggcggga tcgaagccgc tgccgtgtat gccgtcagcg
     3601 gtcgcgtgag catcccggta gtcgcgaggg gtgctaccca tgcgaacgtc tccccctcgt
     3661 cagtgctccg atagagaccc gggcgcgggc cgtagacgat ctgcacgtaa atcgccgttt
     3721 ggtcggggta gctcggcgag accaggattt ggaccggcca agagaggccg aggccttgca
     3781 gaccaactga cggcaacggc tcccaagtaa cgcctccgtc agtagaacga cggacgagca
     3841 cgttgccgct cggctgaacg gtcccgggga gatactcggc cgtcgcacag aaagccgtgc
     3901 gatcctcggc gaagcgcggg gagaacgtga cgacctcgca cacgggttgg tagacgtact
     3961 cctgctgacc ctgcgccggg gaagacgaga gcgtcacggc ggagaccgcg gcgagggcga
     4021 tcgcccgcat cctcatcgga agcgcgcgtc catccgcctc ccccgccagt tccgccatac
     4081 ctggtttgtt ctcgtccgta ggacgtttcc cttcaccgcg gcgaagggcg gcgggacccc
     4141 cgccgcccgg tgccgctcta gttgatccgc cccgcgaacg ggttcgggtc gagttcggac
     4201 ccacagaggt cgaatccctc tgcgcttatc tgaccggccg aggcctgtcc gacgttcacc
     4261 aaggctccct cgatcgtgac gaccccacca atccacgtca cggccacggg gccgctgatc
     4321 ggaaccgcgg cactcgtggg ggccgggcta taggtcgcgg taccgatacc ggctccggtg
     4381 cacgagtgca cgtatgactc gccttcctcg gcctcggcgg tcgttatctc cgtggtcagc
     4441 aaagccctga ccgtgacgcc gcacccaagg cgaccagcgc tcgcgtgacc cgggagaacc
     4501 gagaccgacg ccgtctcctc gcagaagttc gtcggaacgg ttgcgcccgc ggcggtcgcc
     4561 gtcaacgagc aactgcgaag gtgtacaccg gtcgacgtgg gcagcgagca agagaccgag
     4621 gtcccgtcga cgttagcgaa gacgatcgct acggccgccg aagccgtctg tgcatttgcc
     4681 gagggggcca ccgcggcccc gatgagcgtg accgctgccg cgaccacgcc cgcgagtttc
     4741 tttttcatcc ccacttcctt tccgccctta ctgcgcgtcc gatgctccca aggcctcgaa
     4801 cgcccgccta gcgttcagat ccgcgcgtcg aacggcaccc ctccttcagt caactccgcc
     4861 tatacagggc gttgtatcac gcttgctctg cttgggacag ttcggagcac ctgtccagag
     4921 ctgacgcctc tcgcctaggc cctctccagg gcctccagta gcctcgctac gtcggtggcg
     4981 tcgttgtaga cgtgtggcga gaccctgatc gagtcgcctc tcacactcac gtagacgttc
     5041 gcctcggcca ggaggttcgg gagcttttcg gggatcccac ccgcgcggcg caggccgagc
     5101 atgtggcgcg accggctctg acggggcgct acgtcccagc ccaggctcgt cgcgccgtcg
     5161 gctatctcgt ccgtcaacgc ctcgatgtac gactcgatct cttccgcacc ccacgacagc
     5221 agctggtcca gggcggctat ccccatcggc atcaggtgga agttcgcccg ctcgcccatg
     5281 tcgtaccggc gcgcccccag ggcgaactcg tcggtgtaac ggacgaggcc ggcgaagtcc
     5341 tcggcccccg cacgcgtcag ccagttgcgc tccagcgggt cgccgtcgcg ccgttcgggc
     5401 gcgacccagg cgtagccgag gctgaacggg ccgagcatcc acttgtagcc cgcggcgaag
     5461 agccagtccg gccggacggc accgacgtcc agaggacgga cgccgagaga ttgcgtcgcg
     5521 tcgacgacga gcgcggcgcc ggcagcgcgc gcggcctcgc cgacacgcac gaggtcgacc
     5581 acggtcccgt cggtccagtg gcagctcggc accgcaacga tcccggtgta cacgtctatc
     5641 cgatcgatca cggcgcgcgt ccagtccgag tcggccgggc gcgggacggt tacgagcccg
     5701 ccgcccgcac gcgccgctgc gtcgcgccac tcgtacacgt tcgaggggaa ctgctcctcc
     5761 agcaccacga ccttctggcc ccgggcgagc ggcatgttct tcgcagcggt cgcgaccccg
     5821 tagctgacgg ccgggattat cgccacgccg tctgcgtcac cgccgacgag ctgcgcgaac
     5881 ctctcccgcg ccgactcggc gccggagaaa aagtccggcg gggagatcgt ccacggctgc
     5941 ccggtccgcg ccagggcctc ctgcccggcg gcatcgacgc tgtggagccg ggggccgagg
     6001 taggcggcgt tcaggtacgt gacgccggcg gggatgtcga acaggtgccg ctgcgaatcc
     6061 atctacgcct ccaacgacct cgccaggtcg gtaatcgcct tcggataccg atactgcaac
     6121 ccaccgtgag agccgtcgaa cagcacgagt tcgtgctcga cgccgacggc ggtcagctcc
     6181 ttcgagaaag cgatcgcgcc gaggtcgagg aagtactcgt cggacctccc cgcgtcgagg
     6241 tagacgtgct tcatcgaggc gagggcctcc atcctgcggg gcgcgacgcg gaccgggtcc
     6301 cactcgagcc agcgctccca cacgtcgggg atcagccgtc cggtctcgat ctcaaacggg
     6361 aggacgacct tgcccggctc gttctcgtcg ggcgagtagc acgccgccat cgcgtaggag
     6421 tcgagagggt ggccgaacag gccgaagtcg aagcgctccg cggagcggac cctctcccag
     6481 aagacgtcgt aggagccttc gaagcgatcc cgcaggatcc gtgcggtgtc gcggaactcc
     6541 ggctggtagc agacctcgaa cagcgcgtcg ccggcgtgcg acgcgaggcc cccgaacacg
     6601 tcgggacgga gcatcgggac catcatcgcc ccgtatccgc cgctggactt gccggtgatc
     6661 ccgcggtggt cgcggctcgc cgcggtcgga tagcgcgagt cgacgaacgg caccacctcg
     6721 tcgcacaggt agtccatgta gcggcccgtg gcggcggagt tcacgaactg cgatccgccg
     6781 taggaggtcc acgcgtcgac gaacacgacg acagctcgcg gagccccgtc ggcacagaac
     6841 gccgcgtcga cgcgctcgat catcgtcggc tcgaacgcgc tgcggccgag ccacatgtcg
     6901 agctgaccgg tcatcccctg gatgacgtag accgacggat aggcatgatc cgacgacgtt
     6961 tcgacgcccg gcggcaggta cacgtacagc ggccggcgag cgggatcacc gagtgggttg
     7021 tccgcgagta gctcggactc gaccgtgagc ttctcgaacg tcccccgtat gtctcgctcc
     7081 caaggggggc cggggagccc ctcgacctcg cggagcgtca cgaccccccg cggatctccg
     7141 ccacgatctc ggcgacctcg gtcgggttct tgccgactcg gatccccgcc gcctccagcg
     7201 cctccgcctt cgcctgcgcg gtgcccttcg accccgagac gatcgcgccc gcgtgcccca
     7261 tgcgcttgcc gggcggtgcg gtaaagcctg cgatgtaggc gacgacgggc ttgctcatgt
     7321 tcgcctggac ccacgcggcg gcccgttcct cggcgtcgcc gccgatctcg ccgacgagca
     7381 ccgcgagctc ggtctccggg tcggcctcga agcgcgtgag cacgtcgatg aactgcgacc
     7441 ccggtagggg gtcgccgccg atgccgacgc aggtggattg cccgatgccg cgctgggtga
     7501 gctcgtggac gatctgatag gtgagcgtgc ccgagcgcga gacgagaccc acgggacccg
     7561 gcatgcagat ctcgcccggg atgatcccga cgttcgcttt ccccggcgag atgagacccg
     7621 gacagttcgg cccgatcagc gcgacgtcgc ggtccccgcg ccgcaggtag ggaaccgtcc
     7681 gcgccatgtc catcgcgggg atgccctcgg tgatcgccac gatcacgtcg atgccggcgt
     7741 cgtaggactc gaggatcgcg tcggccgcga agcgcggcgg cacgaagacc atcgacgtgt
     7801 tcgcgccggt ctgctcgacc gcgtcgcgca cggtgtcgaa gaccggtacg ccgtcgacgt
     7861 cgctcccgcc cttgcccggc gtgacgccgg cgacgaggtt cgtcccgtac tgcttgttgc
     7921 ggccggcgtg gaaggagccc tcacggccgg taagtccctg gacgacgagc ttcgtcgagt
     7981 cgtcgatgag gatcgccatt agttcgcacc tccgtgagac aggtccacgg cgagctgcgc
     8041 ggcccccagc atcgtgtcgg acgtcttcag catcggatgc ggcgcgtcgg ccagcatctt
     8101 gcggccctgc tccgcgttcg tcccgtcgag ccgcacgacg atcgggacct tcacgtcgag
     8161 gcggccgagc gcgtcgatga tgccttccgc gacgaggtca caacgcgtga tcccaccgaa
     8221 gatgttgatc agcacgcttc ggacgtcggg gttcgacgtg atgacctcga gcgcggacga
     8281 gagcagctcg gcccccgcgc cgcctccgac gtcgaggaag tttgccgcct cgccaccggc
     8341 ggccttgacg acgtcgagcg tcgacatcac gagccccgca ccgttgccga tgatcccgac
     8401 gtcgccgtcg agcttgatga agttgaggcc cttctccttc gcgatgcgtt cctgctcggg
     8461 gatctcgtgc gccgccttca gctggtcgaa gacggggtgg cggtaggccg cggagtcgtc
     8521 gagcgacacc ttcgcgtcca gcgcgacgac gcggccctcg ccggtgagca cgagcgggtt
     8581 gacctcgacc agagaggcgt cgagctcgac gaaggcccgg tacagcttcg gcagcagcga
     8641 gatggcgtcc ttgcgcgccg cggcatccag gccggagccg aacaccatct cgcgggcgtg
     8701 gaagtcggac ataccgagca acgggtcgat gtggaccatc gcgatcgcgt cggggtccgt
     8761 gtccgcgacc tcctcgatgt cgacgccgcc cttcgcgctc atcatcccga ggaagttgcg
     8821 gttgctgcgg tccagcagga acgagcagta gtactcggcc ttgatgtcgc cggcgcgctc
     8881 gacgagcacg cgcttgaccg tgtggccctt gatgtccatg ccgaggatct tcgacgcgac
     8941 ctcgcgcgcc tcgtcgggcg acccggcgag cttgatccca ccggccttcc cgcggccccc
     9001 gacctggacc tgggccttga cgacgacctt gccgccgaga cccttcgcgg cctcctccgc
     9061 ctcgtccggc gtggtggcca cgcgcccttc cggcacgggt acctcgaact tccggagaag
     9121 ctccttcccc tggtactcga acaggtccac tagccggccg cctcctcgtt ttccgccgat
     9181 acgtggtccc ggacccattc gacgatcgtc ttcgtcgtcg tgcccggcgt gaagatctcg
     9241 ctgatgcccg actccttcag cttcgggatg tcggcgtcgg ggatgatccc gcccccgaac
     9301 acgagcatgc cctcgccgcc ctgctcctcg aggagctgcg cgacgcgcgg gaagagcgtc
     9361 atgtgagcgc ccgagaggca agagaggccg accgcgtcga cgtcttcctg gatcgccgcc
     9421 gaagcgatct gctcgggggt ctggtggagg cccgtgtaga tgacctccat gcccgcgtcg
     9481 cgcagcgcgc gggcgactac cttgatgccg cggtcgtggc cgtcgaggcc cggcttggca
     9541 accaatatcc gtggtcttcc ggagggcacg agtgatcgct ccttggcgag aggtgagacg
     9601 gtcgtgcgta gagtctcggg aacggaccga ggttagcagg tcgaagggga gataaacctt
     9661 gccggagacg gcacctgagg cgattgaaac cctgatcgac ggcgggtcgt tcgagcccgc
     9721 gggcgacgcc gttccggccg gcgacccgct cggctatccc ggctacacgg acgcgctcgc
     9781 ccgcgcacgg gagcggtccg gaacggacga gtcggtcgtc accggcgcgg cgacgatcgg
     9841 tggcatcgcg gtcgaggtag cggcgttcga gttccctttc atgggcggga gcatgggggc
     9901 cgcggccggc gagctgctgg cgcgttcgat ggaacgagcc gcggcgcgcg gcgtcccgtt
     9961 cgtgctgcgc accgcgaccg gcggtgcacg catgcaggag ggcatggcgt cactcgtcca
    10021 gatgccgaag gtcgcgggag cgcggatcga gctgggagag gcgcggcagc cgttcgtagc
    10081 ggtgctcggc aacccgacga ccggcggagt cctcgcgggg ctcgccgcgc tcgccgacgt
    10141 gacgatcgcg gagcgcggcg cgaccgtcgg cttcgcgggc ccgcgcgtcg cggagagggc
    10201 gaccggccgt cccctctcga agacttcgca taccgccgag gccgcgctcg acgccgggct
    10261 ggtcgacgcg gtcgtcgaca cgtcgtccgt tcccgctgtg gtcgagtgga tcctctcgat
    10321 gctgacgtcg cgcggagacg acggcgagca caccccgccc gagacgaccg gcgaccacct
    10381 cgacgcgtgg gaggcgttcc aacgggtgcg ttcgccggag tggccacagt cggttctcct
    10441 cgcggcacag atgggtggcc cggcgttcga gctgcgcggc gaccgagccg gctccgacga
    10501 ccggggctgc gtagcgttcc tcgcacaggt cgacggccga ggtgcgctca tcctggctct
    10561 ccagcgcggc gtcgctcccg gtgccgcgga ctaccgcaag gcgatcaggt gcgtgaggat
    10621 cgccgggcgt gtcggactgc cgatcgtgac gctcgtcgac accggagggg ccgatccctc
    10681 cgaggggtcc gagcgacacg ggatcgcatg ggcgatcgcc gagctgttcg atgcgatcct
    10741 gtcggctccc gttcccgtcg tgtcggtgct gatgggagaa ggcggcagcg gcggggccct
    10801 cgcgttcgcg tgcggcgacg cgctccttgc attcgaggag accgcgttcg ccgtgatcgc
    10861 gccggagctc gccgccgaga tcctgtggcg agacgcgggg agggccgagg aagcggccaa
    10921 gctcttgaag atgggggccg ccgacctggc gcggctcggc atctgcgacg aggtgctcg
//
LOCUS       DASQDO010000546         4441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_363090, whole genome shotgun sequence.
ACCESSION   DASQDO010000546 DASQDO010000000
VERSION     DASQDO010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363090"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1843)
                     /locus_tag="VEV43_15040"
     CDS             complement(293..1843)
                     /locus_tag="VEV43_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYP24882.1"
                     /translation="MSPSDVSPSPLESFDPRTGKVVRTVEATPPAEVAEVVARARKVA
                     PEWAAIPAQGRVRILREVRHRIARRIDDMVDAVSTECGKPPAEALAADVMPIPIMLMY
                     LERIAPRTLRDEHPGRFLGPVLGTGGRVEWRPFGVVGAITPWNYPMLNSFAAFAPALF
                     AGNTVVVKPSEVTPQCGEVLRDLLDPLPPGVATIVQGGRDVGAALVDAPCDKISFIGS
                     PATGRKICEAAAAHLTPVVMELGGKDAAIVCADADLDKASSGLVWSSFMNAGQTCASV
                     ERLYVVDDVADEFQERFLDKLSHVRRGTDMGPLTFRPQLDTVKRHVADAVEKGATVAA
                     GGNAPDEPGLWFEPTVLTGVTTDMDVVRSETFGPVVSITRVRDENEAVRRANEEAVNL
                     TASVWTRSRKRADAIAAELQAGTITANFHGESLAHPWFPWGGTGESGFGRLSGRYGIR
                     EFVMPVNVSRSSTPWLPRLWWYPYTDNVQRSMRAVTELIASPELGTKAKALGAVVTSV
                     AKVVREKI"
     gene            1999..2724
                     /locus_tag="VEV43_15045"
     CDS             1999..2724
                     /locus_tag="VEV43_15045"
                     /inference="COORDINATES: protein motif:HMM:NF020470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1842 domain-containing protein"
                     /protein_id="HYP24883.1"
                     /translation="MTATAQSIHVGLFHVTYEVGSGMPGAPMLALNLAVDTPSKHVTG
                     LARVTQVTAPPFPVTTSATNGNYTYMATMKSTHILVVATGYPVISWPAGGGIGPVIQP
                     NFELRMVLESDWSSGTASFKYYDGTGWHSIDNVPVKKTDVVDLAAAGLPEAILYPDTV
                     EATWSDGVLVIRASGVEDGYTDIHIDVSPADIWPPIYQVVGRETPAIGSFPYDVSRSF
                     PMEDKPDAINFSMKGGDQKIPVN"
     gene            2892..>4441
                     /locus_tag="VEV43_15050"
     CDS             2892..>4441
                     /locus_tag="VEV43_15050"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HYP24884.1"
                     /translation="MSAGVVAGVAPPAAAATFTVDTTVDGVDADPGDGTCATASGACT
                     LRAAVGEANALAGTDTITLSAGTYQLTIPNTEPDVPDDNSRGDLDVFGDSLTITGASA
                     ATTAIVGVQGDRGDRIIHVVGQNGPQHLTITGVTIRGGQAGFSGLGGGILVSGDDDEL
                     TLTSSVVADNADGGSAGGGIAVSNGGRFTMTRSVVRDNAGGCSGGGLALTGPSQIVES
                     TISGNTAECGGGGIVASASAEEPDAARSPTTIVNSTISGNRSQQAGGGVLSSSPVTFT
                     NVTITSNRAGGNVEGEAPGGGVYVGGSSENPDPVRFENTVLANNASLTGTQNNCGDET
                     GGNGLTSGGGNAESGTSCNLTGNGDQSDARLELGPLANNGGPTPTHALGATSHAIDTG
                     LADCPGTDQRGVLRPKDGDEDGTATCDAGAFELDPDAAPSDTTPPGITNVKDAPDPFT
                     PAGRTKKSTKISFTLSEAATVTITIENRAGTVVRTVLNAAQKAAGSVAVRWNGRTTQR
                     KVVKPGRYT"
BASE COUNT          717 a         1583 c         1548 g          593 t
ORIGIN      
        1 acgatcgaag gcggtgcggg cgacgacacg atcacgtccg ggccgcgcga gccctacgag
       61 ttcgaggacg aagacgcagt ctccggaggc cccggcgccg acacgatcga cggaggggtc
      121 ggggccgacg agctcttcgg cgacgacggc aacgacacca tcgacggcgg tgcgggcgac
      181 gaccgcctcg acggcggcgc cggcaacgac gagctcgacg gaggcgcggg gaccgacgtg
      241 tgcgaggagg gcgagagcaa cgcgcgttgc gaggggaccc cgcccctcct gcctagatct
      301 tctcgcgcac taccttcgcc accgacgtca ctaccgcgcc cagggccttc gctttcgtgc
      361 ccagctccgg cgacgcgatc agctccgtca cggcgcgcat cgagcgctgc acgttgtccg
      421 tgtacggata ccaccacagc cgcgggagcc acggcgtcga gctccgcgac acgttcaccg
      481 gcatcacgaa ctcgcggatg ccgtagcgcc cgctgaggcg cccgaacccc gactcacccg
      541 taccccccca cgggaaccac gggtgcgcca gcgactcgcc gtggaagttg gccgtgatcg
      601 tgccggcctg aagctccgcg gcgatcgcgt cggcgcgctt cctgctgcgc gtccacaccg
      661 acgcagtgag gttcaccgct tcctcgttcg cgcgccgcac ggcctcgttc tcgtcgcgga
      721 cacgcgtgat cgagacgacg gggccgaacg tctccgaccg cacgacgtcc atgtccgtcg
      781 tcacgccggt aagcacggtc ggctcgaacc acaggccggg ctcgtcgggc gcgttccctc
      841 cggctgcgac cgtcgcgccc ttctcgacgg cgtcggcgac gtgccgcttg acggtgtcga
      901 gctgcggccg gaaggtcagc ggacccatgt cggtcccgcg ccggacgtgc gacagcttgt
      961 ccaggaagcg ctcctggaac tcgtcggcga cgtcgtcgac gacgtagagg cgctcgacgg
     1021 acgcgcacgt ctggcccgcg ttcatgaacg acgaccacac gagacccgac gacgctttgt
     1081 cgaggtcggc gtcggcgcag acgatcgctg cgtccttgcc cccgagctcc atcacgaccg
     1141 gcgtcaggtg cgccgcggcg gcctcgcaga tcttgcggcc cgtcgccggc gagccgatga
     1201 acgagatctt gtcgcagggg gcgtccacga gcgccgcgcc gacgtcgcgg cccccctgca
     1261 cgatcgtcgc gactcccggc gggagcggat cgaggaggtc gcgcagcacc tcgccgcatt
     1321 gcggggtgac ctccgacggc ttgaccacca ccgtgttgcc ggcgaacaga gcgggcgcga
     1381 acgcggcgaa cgagttcagc atcgggtagt tccacggcgt gatcgcgccg acgactccga
     1441 acggcctcca ctcgacgcgt ccaccggtcc cgagcacggg gcccaggaac cgcccgggat
     1501 gctcgtcgcg cagcgtgcgg ggcgcgatgc gctcgaggta catcagcatg atcgggatcg
     1561 gcatcacgtc ggcggcgagg gcctcggcgg gcggcttccc gcactcggtc gacaccgcgt
     1621 cgaccatgtc gtcgatcctg cgggcgatgc ggtgacggac ctcgcgcagg atccggacgc
     1681 ggccctgcgc cggtatcgcg gcccactcag gcgccacctt ccgcgcgcgc gcgacgacct
     1741 cggcgacctc ggccggcggg gtggcctcga ccgtgcgcac gaccttcccc gttcgggggt
     1801 cgaacgactc gaggggagac ggcgagacgt cgctggggct catgtcgatt cctccgggcg
     1861 agggacgagg ctgtcctgaa actaccgcgc caacccggca gctaacccct tgtcacggcg
     1921 tgttccacgt ggttacctcg acacgtacag ccaagagcgc tcgcggcacc agcttcgtcc
     1981 aagggatggg aggcggacat gaccgcgacc gcacaatcga tccacgtcgg gctgttccac
     2041 gtgacctacg aggtgggctc cggcatgccg ggcgcgccga tgctcgcgct gaacctggcc
     2101 gtggacaccc cgagcaagca cgtgaccggg ctcgctcgcg tcacccaggt gacggcaccc
     2161 ccgttccccg tgacgacctc ggcgaccaac ggcaactaca cgtacatggc gacgatgaag
     2221 tcgacgcaca tcctggtggt cgccaccggg tacccggtga tctcgtggcc ggccggcggc
     2281 ggcatcggtc ctgtgatcca gccgaacttc gagctgcgaa tggtcctcga gtccgactgg
     2341 tcctcgggca cggcgagctt caagtactac gacggcacgg ggtggcattc gatcgacaac
     2401 gtgcccgtga agaagaccga cgtcgtagac ctcgcggcgg ccgggctgcc ggaggcgatc
     2461 ctctatcccg acacggtgga ggccacctgg tccgacggcg tcttggtgat acgcgcctcc
     2521 ggcgtggaag acggctacac ggatatccac atcgacgtgt cgccggccga catttggccg
     2581 cccatctacc aagtggtcgg gcgggagacc ccggcgatcg ggtcgttccc gtacgacgtg
     2641 tcgcgcagct tcccgatgga ggacaagccc gacgcgatca acttcagcat gaagggtgga
     2701 gaccagaaga tcccagtgaa ctaaggggcg cggtcacgac gagcgggtgt ccgagagcac
     2761 ccgctcgttc gcgcccgatg cccttgtcgc gtgacgggtt ggcgtcgtta actgggcgag
     2821 tccggcgtcc agttgagggt gaggtccaag tggtgtcgtc gcgcaagtcg tcccttgtgt
     2881 tcgctttgct cgtgtccgcg ggcgtcgtgg ccggcgttgc gccgcctgcg gccgccgcca
     2941 ccttcaccgt cgacaccacc gtcgacggcg tcgacgccga tcccggggac gggacctgcg
     3001 cgacggcgtc gggcgcgtgc accttgcgcg ccgcggtcgg agaggcgaac gccctcgcgg
     3061 ggaccgacac gatcacgctg tccgccggga cctaccagct cacgatcccg aacaccgagc
     3121 cggacgtccc cgacgacaac tcgcgcggag acctcgacgt cttcggggac tcgttgacga
     3181 tcacgggggc ctcggcggcg acgacggcca tcgtaggcgt gcagggtgac cgcggcgatc
     3241 gcatcatcca cgtcgtcgga cagaacgggc cgcagcacct gacgatcacc ggcgtgacga
     3301 tccgcggcgg gcaggccggc ttctccgggc tcggaggcgg gatcctcgtc agcggcgacg
     3361 acgacgagct gacgctgacg agctcggtcg tcgcggacaa cgccgacggt ggctccgccg
     3421 gcggaggcat cgcggtgagc aacggcggcc gcttcacgat gacgcgctcc gtcgtccgcg
     3481 acaacgccgg gggctgctcc ggaggcggcc tggccctcac cggtccgtcc cagatcgtcg
     3541 agagcacgat ctccggcaac accgcggagt gcggcggcgg cgggatcgtc gccagcgcgt
     3601 cggccgagga gcccgacgcg gcccggtctc cgacgacgat cgtcaacagc acgatcagcg
     3661 gcaaccggtc acagcaggcc ggcggagggg tcctctcgag cagcccggtg acgttcacca
     3721 acgtcacgat cacgagcaac agggccggcg ggaacgtcga gggcgaggcc cccggcggcg
     3781 gcgtctacgt cggcgggagc agcgagaacc cggacccggt gcggttcgag aacacggtcc
     3841 tcgccaacaa cgcgtcgctc acggggacgc agaacaactg cggcgacgag accggcggga
     3901 acgggctcac ctccggcggc ggcaacgccg agagcggcac ctcctgcaac ctcacgggca
     3961 acggcgacca gtcggacgcg cgcctcgagc tcggccccct cgcgaacaac gggggcccga
     4021 cgccgacgca tgcgctgggc gccaccagcc acgcgatcga cacgggtctg gccgactgcc
     4081 cgggcaccga ccagcgcggg gtgctgcgcc cgaaggacgg ggacgaggac ggcaccgcga
     4141 cttgcgacgc cggcgctttc gagctcgatc cggacgcggc cccgtccgac acgacgcctc
     4201 cgggcatcac gaacgtcaag gacgcgccgg acccgttcac gcctgcgggg cgcacgaaga
     4261 agtccacgaa gatctccttc acgctctcgg aggcggcgac cgtgacgatc acgatcgaga
     4321 accgggccgg gacggtcgtt cgcacggtgc tgaatgcggc gcagaaggcc gctggctccg
     4381 tcgccgttcg ctggaacggc cggacgacgc agcgcaaggt cgtgaagccc ggccgctaca
     4441 c
//
LOCUS       DASQDO010000547         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_914245, whole genome shotgun sequence.
ACCESSION   DASQDO010000547 DASQDO010000000
VERSION     DASQDO010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914245"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VEV43_15055"
     CDS             complement(<1..930)
                     /locus_tag="VEV43_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYP24885.1"
                     /translation="MADSPYWNPRHETMPRDQLDALQLHKLRRTLEWAEKQVPFHAER
                     LSRAGVGAQDVQSLDDLRRLPFMTRDEWMDAQLRQPPYGPILAAPKETAIRYHMTSGT
                     TGRTPIRVLDGMKDWEWIAEMWCYGFWGFGVRPADTVFFAFSYGTFVGFWGAHYACEK
                     IGCLVLPGGNMTTEARVRQIMDMDATVVCSTPTYALRMAQEAASMGVDLANSPVERLI
                     LSGEPAGSIPATKKLIEQQWGAKAADTAGMTELGTIMMFECDHQPGGTHIIEPHYIEE
                     VIDPESGDPVPYGEQGERVVTSFGRGFIPVIRYR"
     gene            1145..2242
                     /locus_tag="VEV43_15060"
     CDS             1145..2242
                     /locus_tag="VEV43_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP24886.1"
                     /translation="MERAALLDEVRGIARQYLKPVADAGTPGRVNRPLLATLAEHGLL
                     PRLFPSDGSVSARTMCLLREAIAYVSPEAETALALQGLGSYPILQSGSGHLSGRWIPE
                     VAAGRAVAAFALTEPGAGSDAAALALRAEPDSDGFRLTGEKRWISNAPDADVYTVFAR
                     TGEGPGARGVSAFAVEGSSPGLSGESLELVAPHPIGTLVFDGVLVPRSHILGQPGRGF
                     EVAMRTLDLFRPSVGAFAVGMAQAALDAAVAHAAERHAFERPLKDFQAVSHRLADMAT
                     KLEAARLLVYRAADKYDAAEPVTEASAMAKLFATEAAQEIVDAAIQVHGAQALEKGHM
                     LEHLYREVRATRIYEGTSEIQRNVIARSLFG"
     gene            complement(2239..2634)
                     /locus_tag="VEV43_15065"
     CDS             complement(2239..2634)
                     /locus_tag="VEV43_15065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYP24887.1"
                     /translation="MEGSVTPHRIVNPEELAPPEGFAHAVVAAPGRTVYLGGQIGEGS
                     TMPAQFAAAARNVVVALRAAGAEPHHLVSLTIFVTDVNAYRKALAPIGAAYRKNFGRH
                     YPAMALFGVSRLFEPDALVELVGIAVVPD"
     gene            complement(2619..3446)
                     /locus_tag="VEV43_15070"
     CDS             complement(2619..3446)
                     /locus_tag="VEV43_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HYP24888.1"
                     /translation="MSSWRASAPFTREWEHFDFDVRDGIGTITFARPEVLNALTFDVY
                     ADLRDLLAELRLRDDVRVLVVTGTGRGFCSGGDVHEIIGELLKMESRQLLDFTRMTGA
                     VVQNMRDCPIPIVAAINGIAAGAGSVIALAADFRLMARSASFAFLFTKVGLAGADMGS
                     AYLLPRLVGFGRATELLILGDKVTSERALEIGLASSVVPDEELAGAAAELAERLAAGP
                     SFAYGTTKMLLSRELDADLAGALELEATTQALLMTTEDHREFYRAFTEGRAPQWKGR"
     gene            complement(3443..>4354)
                     /locus_tag="VEV43_15075"
     CDS             complement(3443..>4354)
                     /locus_tag="VEV43_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP24889.1"
                     /translation="LEAGDSGFRSFVSVQGSLAMFPIWRYGSEEQKQEWLPRMAAGEA
                     IGCVGLTEPDSGSDPGSMRTTARRDGSDWVLNGNKMWITNGGVADVAVVWARTDEGIR
                     GFVVPAGSRGFTARNIHRKLSLRASVTSELILDDCRVPGDAVLPGVTGLKGPLSCLTE
                     ARFGIVWGAMGAARACYESALEYSKTRIQFGKPIGAFQLTQRKLVDMLVEIEKGMLVA
                     LHLGRMKDQGTATPQQVSFGKLNNVREALAIAREARTILGANGVTLEYPVIRHMNNLE
                     SVLTYEGTSEIHTLVLGKEITGLDAFA"
BASE COUNT          618 a         1531 c         1554 g          651 t
ORIGIN      
        1 ccggtaccgg atgaccggga tgaacccgcg gccgaacgac gtgacgacgc gctctccctg
       61 ctcgccgtaa gggaccgggt caccgctctc ggggtcgatg acctcttcga tgtaatgcgg
      121 ctcgatgatg tgcgtcccgc ccggctggtg gtcgcactcg aacatcatga tcgtgccgag
      181 ctcggtcatc cccgccgtgt cggcggcctt cgcgccccac tgctgctcga tgagcttctt
      241 ggtcgccggt atcgagccgg ccggctcgcc ggagaggatt agcctctcga cagggctgtt
      301 cgcgaggtcg acccccatcg acgccgcctc ctgcgccatc cgcagcgcgt aggtcggcgt
      361 cgagcagacg acggtcgcgt ccatgtccat gatctggcgc acccgggctt cggtcgtcat
      421 gttcccgccc ggcagcacga ggcagccgat cttctcgcac gcgtagtgcg cgccccagaa
      481 ccccacgaac gtcccgtacg agaaggcgaa gaagaccgtg tcggcgggcc ggacgccgaa
      541 gccccagaag ccgtagcacc acatctcggc gatccactcc cagtccttca tcccgtcgag
      601 cacgcggatc ggcgtgcgcc cggtcgtgcc cgaggtcatg tggtagcgga tggccgtctc
      661 cttcggcgcg gcgaggatcg ggccgtacgg cggctgccgg agctgggcgt ccatccactc
      721 gtcccgcgtc atgaacggga gccggcggag gtcgtcgagg ctctggacgt cctgggcgcc
      781 gaccccggca cggctgagcc gctcggcgtg gaacgggacc tgcttctcgg cccactcgag
      841 agtgcgccgg agcttgtgga gctgaagcgc gtcgagctga tcgcgcggca tggtctcgtg
      901 ccgtgggttc cagtaaggag agtcagccat ccggcctccc gggtcgagcg ctccgacggg
      961 gggagcctac gcccacgccc cgcgccgccg gcggtccggc cgaggccgtc ccgcgctgag
     1021 gccgtggttc gtctggtcaa tgtggttggc gaggttggaa gtgcaacctc gttgaccaca
     1081 cgtaccagac gtaccagagg gtccgggagg aaagggtgcg cgccgggcgg ctacgctgcg
     1141 gcgcgtggag agggccgcgc tgctcgacga ggtacggggg atcgcccggc aatacctgaa
     1201 gccggtcgca gacgccggca cgcccggccg cgtgaaccgg ccgctgctgg cgacgctcgc
     1261 cgagcacggc ttgttgcccc ggctctttcc ttccgacggg tcggtctcgg cccgcacgat
     1321 gtgtctgctg cgcgaggcga tcgcctacgt ctcgcccgag gccgagacgg cgctggcgct
     1381 gcaggggctc ggctcctacc cgatcctcca gtccgggtcc ggccaccttt cggggcgctg
     1441 gatcccggag gtcgcggccg gccgcgccgt ggccgcgttc gccttgaccg agccgggggc
     1501 cgggtcggac gccgccgcgc tcgcgctgcg ggccgagccg gactccgacg ggttccggct
     1561 cacgggtgag aagcgctgga tctcgaacgc ccccgacgcg gacgtctaca cggtgttcgc
     1621 ccggaccggc gagggcccgg gcgcgagagg cgtctcggcg ttcgccgtcg aggggtcgag
     1681 cccgggcctg agcggcgagt cgctcgagct cgtggcgccg catccgatcg ggacgctcgt
     1741 gttcgacggg gtgctcgtgc cccgctccca catcctcggg cagccgggcc gggggttcga
     1801 ggtcgccatg cggaccctcg acctgttccg gccgagcgtc ggcgcgttcg ccgtcggcat
     1861 ggcgcaggcc gctctcgacg cggccgtcgc gcacgccgcc gaacggcacg cgttcgagcg
     1921 gccgctgaag gacttccagg cggtctcgca ccggctggcc gacatggcga cgaagctcga
     1981 ggctgctcga ctgctcgtgt accgggccgc cgacaagtac gacgcggccg aacctgtcac
     2041 ggaggcgtcg gcgatggcga agctgttcgc gaccgaggcg gcccaggaga tcgtcgacgc
     2101 cgcgatccag gtccacggcg cgcaggcgct ggagaagggc cacatgctcg agcacctcta
     2161 ccgggaagtg cgggcgacgc ggatctacga ggggacttcg gagatccagc gcaacgtgat
     2221 cgcccggtcg ctgttcggct agtccgggac gacggcgatg ccgacgagct ctacgagcgc
     2281 gtccggctcg aacagacggg acaccccgaa cagcgccatc gcggggtagt gcctgccgaa
     2341 gttcttccgg taggcggcgc cgatgggggc cagggccttg cgataggcgt tgacgtccgt
     2401 gacgaagatc gtgagcgaga cgaggtggtg cggctcggcc cccgcagcgc gcagcgcgac
     2461 gacgacgttc ctcgcggccg ccgcgaattg cgccggcatc gtcgacccct cgccgatctg
     2521 gccgccgagg tagaccgtgc ggccgggggc cgcgacgacc gcgtgggcga agccttccgg
     2581 cggggccagc tcctccgggt tcacgatccg gtgcggcgtc accgaccctt ccactgcggc
     2641 gcccttccct cggtgaacgc ccggtagaac tcgcggtggt cctcggtcgt catcagcagg
     2701 gcctgggtcg tcgcctcgag ctcgagggcc ccggccaggt ctgcgtcgag ctcccgcgac
     2761 aggagcatct tcgtcgtccc gtacgcgaac gagggcccgg ccgccagccg ctcggcgagc
     2821 tcggccgcgg cccccgcgag ctcttcgtcc gggacgaccg acgaagcgag ccctatctcg
     2881 agtgctcgtt cggacgtcac cttgtcgccg aggatcagca gctcggtagc tcgaccgaag
     2941 ccgacgaggc gcggcagcag gtaggccgag cccatgtcgg cgccggcgag gccgaccttc
     3001 gtgaacagga acgcgaacga cgcgctccgg gccatcagcc ggaagtccgc ggcgagggcg
     3061 atgaccgacc cggcccccgc tgcgatcccg ttgatcgccg cgacgatcgg gatcgggcag
     3121 tcccgcatgt tctgcacgac cgcaccggtc atccgcgtga agtcgaggag ctggcgcgac
     3181 tccatcttca acagctcgcc gatgatctcg tgcacgtcgc cgccggagca gaagccgcgc
     3241 ccggtcccgg tgaccacgag gacgcggacg tcgtcgcgca gccggagctc cgcgagcagg
     3301 tcacgcaggt cggcgtagac gtcgaacgtc agtgcgttga ggacctcggg gcgggcgaac
     3361 gtgatcgtgc cgatgccgtc gcgcacgtcg aagtcgaagt gctcccactc gcgcgtgaag
     3421 ggggccgagg cccgccagga gctcacgcga acgcgtccag gcccgtgatc tccttgccga
     3481 gcacgagcgt gtggatctcc gacgtcccct cgtaggtgag cacggactcg aggttgttca
     3541 tgtggcggat cacggggtac tcgagcgtga cgccgttcgc gcccaggatc gtcctcgcct
     3601 cgcgtgcgat cgccagggcc tcgcggacgt tgttgagctt gccgaagctg acctgctgcg
     3661 gcgtcgccgt cccctggtcc ttcatgcggc cgaggtggag cgcgaccagc atccccttct
     3721 cgatctcgac cagcatgtca acgagcttgc gctgcgtcag ctggaacgcg ccgatgggct
     3781 tgccgaactg gatacgcgtc ttcgagtact cgagcgcgga ctcgtagcac gcccgcgcgg
     3841 cccccatggc gccccacacg atgccgaagc gcgcctcggt cagacacgac aaggggccct
     3901 tgagaccggt tacgcccggc aggacggcgt cgccggggac gcggcagtcg tcgaggatca
     3961 gctcggacgt gacggacgct cgcagcgaga gcttgcggtg gatgttcctc gccgtgaagc
     4021 cacgcgagcc ggccgggacg acgaagccgc ggatcccctc gtcggtgcgc gcccacacga
     4081 ccgcgacgtc ggcgacgccg ccgttcgtga tccacatctt gttgccgttg aggacccagt
     4141 ccgacccgtc gcggcgggcg gtcgtgcgca tgctccccgg gtcgctcccc gagtcgggct
     4201 cggtcaggcc gacgcagccg atggcctcgc cggcggccat gcgcgggagc cactcctgct
     4261 tctgctcctc cgacccgtac ctccagattg ggaacatcgc gagcgagccc tggacggaga
     4321 cgaagctgcg gaagccgctg tcgccggctt cgag
//
LOCUS       DASQDO010000548         9607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1158455, whole genome shotgun sequence.
ACCESSION   DASQDO010000548 DASQDO010000000
VERSION     DASQDO010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158455"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEV43_15080"
     CDS             complement(<1..374)
                     /locus_tag="VEV43_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYP24890.1"
                     /translation="MRPLRVITLCAAVALLAACGLPRDPESTLRRVSGGTLRVGVTEH
                     DPWVVLGENGPRGVEVELVERFAAEIGADVEWVEGGLEELGGALHVREIDLLIAGLTS
                     RSGVASEGALTHPYLTTQVVIAV"
     gene            414..905
                     /locus_tag="VEV43_15085"
     CDS             414..905
                     /locus_tag="VEV43_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HYP24891.1"
                     /translation="MPVQVAFLRAINVGKRRVSKDRLRQPLVDLGFDDVWTFIASGNV
                     VFRSGAPPADAEPEIEGALTDALGFEVVAFVRPASAIAKVVREQPFSGTEDSVHVGFV
                     KKRVTAPVRRSLEELGNDVDTVTARGTEVYWLARGGMGRATVSGSLLEKTLGGPTTGA
                     SRC"
     gene            complement(928..1218)
                     /locus_tag="VEV43_15090"
     CDS             complement(928..1218)
                     /locus_tag="VEV43_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HYP24892.1"
                     /translation="MSNRISHLFFNEAVRVVEDQGVDPATVDRLFKKCFGHAMGPLET
                     ADLIGLDTVALTLENLRDSYGDDRFAPAALLQEMVRAGKLGKKSGRGFYEYP"
     gene            complement(1243..1794)
                     /locus_tag="VEV43_15095"
                     /pseudo
     CDS             complement(1243..1794)
                     /locus_tag="VEV43_15095"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
     gene            1830..2579
                     /locus_tag="VEV43_15100"
     CDS             1830..2579
                     /locus_tag="VEV43_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24893.1"
                     /translation="MNRIVLAWIALAGFLAGCAGGDDPSPAAAPTPSPVETGFFSGGE
                     CPPAPYDDLPDDAGCATAASDGDARLTVYALLDGEAKPRAWRVRLAGEGAEIDQKLRE
                     ANVWSYPRALGATDVDRDGEREWWLKVADYGSHGAAWGGLHVFVVRGGALVPVTFEGE
                     PLTVDFGGISRLGQGAVCRDGRLVLLRVWARDRQNTRWWVSERTLEIEEGRARLVDTR
                     RRTIAVDSYTDPSLVRNYRVRCDGTTFTPFD"
     gene            2903..3364
                     /locus_tag="VEV43_15105"
     CDS             2903..3364
                     /locus_tag="VEV43_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24894.1"
                     /translation="MINERKHRSKRSALAVVVAAVCATTLLPPTAALAQETSRESAPT
                     ERALIAGVTTCTRGLCVFTATKSATQALNAWLPVAQPAVHALKGICPLLGFSVVGGAI
                     CGAIVFFVGRVYSNARNVIRAAVTNGQCFFIKYVKWGWIPYAMGRTSANCA"
     gene            3432..3884
                     /locus_tag="VEV43_15110"
     CDS             3432..3884
                     /locus_tag="VEV43_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24895.1"
                     /translation="MGESPRKRKRKVLVRRNVGVVAAMLALSLGLAPQASAATLYFGG
                     GTATHTSTFTGSWVASAVTVQVAVTDATDDAYCVHARITPVTTFMPFAGTTLRLPDVC
                     GRGAHRRFTVRIARKFPAVLQSVVVRLCRSVPSGPVPCAASYVDNPLT"
     gene            4216..4803
                     /locus_tag="VEV43_15115"
     CDS             4216..4803
                     /locus_tag="VEV43_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24896.1"
                     /translation="MPPATTLQPVELNADELRSLLVKSLSARGPQVVWVDAGDEVVVD
                     LDATRVALTPGMVLVGLSLTTDAHAHATVSVPVAIGTPERITGLHAVTPRVPEGPVDL
                     VTRWGRSVVAAVWGALVDVVEAMAGAVGEDLDGAPLAPAAIVTDGKTFGVVPQARPTR
                     EQAMTTPEKDDGRKPKGRPTRPEPRDADPYRPKGR"
     gene            4803..>9607
                     /locus_tag="VEV43_15120"
     CDS             4803..>9607
                     /locus_tag="VEV43_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24897.1"
                     /translation="MVQRPSELEDLGIALGLIEGSGSSATLNTEFFTDPRGVLGGTLA
                     DPDRRAALVDLFTDLLGTEGTPHGFDAGTSADETWLPLVEGGPGGLYVLLTPHPPQTT
                     DGVRVSVAGRVQHVDNGVDASASIVLPLICFDNGGLVPLVGTADGPVSIQCRVGGEDG
                     PLIDDDGLVLHAVSVRATIATQALATPPATLQVLVEGLRFGADATPLDVDLADLAGSV
                     GGPAAHLVLAALRAQASTSPELAQLFDLLGLGGAAGIPSLDLGALASDPHGTLTAWLR
                     SVVLGTASRAAWLSKLAGLLGGSVPSPDELCFVDTTATKVCVTVAVDGDPTTGAPRVR
                     IGLLARHEVAGIATGHLRATVNAVDVVLTQPPGVVVFPSARAVLRLEGTAGPLVHGPA
                     ANNRRIEVGSLDAGIELRPDGSIVPVLTANDAIVDLVGFPTLDLTDADAVIGAAEDVA
                     ETALNDLVGDLAGLLNGSPRVRALASLLGLVAPPGPAWAPALVNPASLLADPLREICC
                     YHSRVLAEDGGWGRLLQQLVVLVQPGDDPPPALTGAGTAAEPWRAPFAVDGTKRVDLF
                     ALGRPPDGDHPQLVLGVAVRGATDIGDGKELGLIVRSEVLAATLPRAADCVGEASVTM
                     MPLHEVVLRLGDDLHLDAGAVEVLADRVEVGVRWRPGDDLEPIVRVSDPRVSTGGSFV
                     ELPDIDGDIDLDALGTAGWQLFGDLLGAWFSGGGRSWWRLSALVGLLPGVPDAPQMLP
                     IGDLALPRAWDGLLHLGRGHVTGNGTYDDPWEISTGAAGLPNPVLWLDPDGPPDLRLP
                     RELFPPALSDALDNATTPLDPERLATLLGRAVTLSPTLADVVDEDLAAALRFLRDQLA
                     GTDGLVAAAAQEVTGWNRIDLASALHLGEPAVLDLDAQVPGLPGGSPRVFVSAPLPWA
                     AAWRGQDPARVIDLREAGREPATFDLDHVTAGGSWFVLLPPRADAVTATIADGLEGLR
                     ARLHAAVAAVRDAAGAPVVLVAHATSGHPARLVAADAGLVSDIVTVGTPHSTSAIGWV
                     AQDEGRRALRALHRLRARARGAGADLGPLADLLDGPLAALEGIVAEVDGTDVLRRHVP
                     FPFADYVTPASWPALAAGVTGHAVVATIVPAEVDGALTTLVGRAIEGLATLPAGRTAP
                     THLGIGLRGLRRDGSSLAGEVRVDTRARVDVHLVEITPGDHRAVPRLGIHADISRNGG
                     WLVRPADVSMGRRQDPRVGRAEIDLALDLGDPSPAVSDPAPRLSARIVLHDVTVLGRF
                     HERWVVDDTLLAALADGTSGGTGTLPEVRIVLGMVATALGNVGGTGPVRDLAGLLVAL
                     QVGTIDGNAFDFTTEGLERLLREPDQVVADLLADADRADDFLSALAACTGGTATGDTT
                     MLGTGGLPFGMVGPLGSPSLRLRTSGPVEVAKQVTIDADVTIDATRRAHGSVTVTSPV
                     DVGGLGNPRFVVGIDTATADLVTVAFELDGDGGGADRVALFPDPDLVALARLAGQVIA
                     AEVARQALTWAHAQQPALVGAVLGAVGLLNPPAAPEPVRSLLGLIRDPVAWLTSDVAL
                     GAGGTALTPASVVAAVDAVAALLGQVPAGGVLTLPGGITLTASPDGDRT"
BASE COUNT         1287 a         3455 c         3436 g         1429 t
ORIGIN      
        1 acggcgatca cgacctgcgt cgtcaggtac gggtgggtca gggccccctc cgaggcgacg
       61 cccgagcgcg acgtgagccc ggcgatcagg aggtcgatct cgcggacgtg gagcgcgccc
      121 cccagctcct cgagcccgcc ctcgacccac tcgacatccg cgccgatctc ggcggcgaag
      181 cgttcgacca gctcgacctc cacgcctcga gggccgtttt caccgaggac cacccacggg
      241 tcgtgctcgg tcacgccgac ccgcagcgtc ccgcccgaca cgcgcctgag cgtcgattcg
      301 gggtcgcgcg ggagcccgca cgccgccagc agcgcgacgg cagcgcagag ggtgatcacc
      361 cggagcggtc gcacggtacg gattctccta cgtcgactgc ctagactcgc cgcatgccgg
      421 tgcaggtcgc gttcttacga gcgatcaacg tgggcaagcg gcgcgtttcg aaggaccgct
      481 tgcggcagcc tctcgtcgac ctcggcttcg acgacgtgtg gacgttcatc gcgagcggca
      541 acgtcgtctt ccggtcgggc gcgccgcccg cggacgccga gcccgagatc gagggggcgc
      601 tcacggatgc gctcgggttc gaggtcgtcg cgttcgtccg acctgcctcc gcaatcgcga
      661 aggtcgtgag agagcagccg ttctccggca cggaggactc ggtgcacgtc ggcttcgtga
      721 agaagcgagt gaccgcgccg gtacgccgct ccctcgaaga gctcggcaac gacgtcgaca
      781 ccgtgaccgc tcgggggacc gaggtctact ggctggcgcg cgggggcatg gggcgcgcga
      841 ccgtgtcggg atccctgctg gagaagacgc tcggggggcc gacgacggga gcctcaagat
      901 gctgacgcgg ttggaggcga agctcagcta gggatactcg tagaagcccc ggcccgactt
      961 cttgccgagc ttgccggccc ggaccatctc ttgcaggaga gcggccggcg cgaagcgatc
     1021 gtcgccgtag ctgtcgcgga ggttctcgag cgtgagggcg acggtgtcga ggccgatgag
     1081 gtcggccgtc tcgagggggc ccatcgcgtg gccgaagcac ttcttgaaga gccggtcgac
     1141 ggtcgcgggg tcgacgcctt ggtcctcgac gacgcgcacc gcctcgttga agaagaggtg
     1201 ggagatccgg ttgctcacga agccggggaa gtcgtcgacg acgatgcctt ccttgtccag
     1261 cgactcgagg aacgcggcga cgtagtcgag cgtctcctgc gacgtgtccg ctccccggat
     1321 cacttcgacg gcgtccttca ggtacggcgg gttcatgaag tggatgccgg ctatccggtc
     1381 ggggcgtgac gtctccgcgg cgacgcgggc gatcgggatg ctcgacgtgt tgacgccgaa
     1441 gcgagcttcc ggagaggcga ccgcgtcgag cgatctgtag acggtctgct tgaccgtcca
     1501 gtcctcgggg acgttctcga cgacgaactc acaacccgcg acggactcga tctcggtcga
     1561 ggtctcgagg gccgcgtgca cgtcgtcggg cttcatcgcc gggaggccgc gccggagcat
     1621 cggcgcgagc cggacggtct ggaggatcga gtcgcgggcc ttgtcgagct gatcctgcga
     1681 gacgtcgacg aggatcacgt gcattccgtg gaggacgagg tcgatcgcga cgccggtccc
     1741 gatcgagccc gcgccgatca cgccgatggt cctctgctcg ccgtgtgtca tccggcctga
     1801 accttgccac gtgttgtact ggagcgacga tgaaccggat cgtcctcgcc tggatcgcgc
     1861 tcgccggctt cttggccggg tgcgccgggg gagacgatcc gtcccctgcg gccgcgccca
     1921 cgccgtcgcc cgtggagacc gggttcttca gcggcggcga gtgcccgccg gcgccgtacg
     1981 acgatctccc ggacgacgcg ggatgcgcca ccgcggcaag cgacggtgat gcgcggctga
     2041 cggtgtacgc acttctcgac ggcgaggcga agccgcgggc ctggcgcgtc cggttggccg
     2101 gcgagggcgc ggagatcgac cagaagctgc gcgaggcgaa cgtgtggagc tacccgcgcg
     2161 cgctgggcgc caccgacgtc gaccgcgacg gcgaacgcga gtggtggctg aaggtcgccg
     2221 actacgggag ccatggcgcc gcgtggggtg ggctgcacgt cttcgtcgtg cgaggcggtg
     2281 cgctggtccc ggtcacgttc gaaggcgaac cgctgacggt cgacttcggc ggtatctcca
     2341 ggctcggcca aggcgcggtg tgccgggacg gccgtctggt gctgctacgc gtgtgggcac
     2401 gcgatcgaca gaacacgcgc tggtgggtgt cggagcggac gctggagatc gaggagggcc
     2461 gggcgcgcct ggtcgacacc cgccgccgaa cgatagccgt cgacagctac accgacccgt
     2521 ccctcgtgcg caactaccgg gtccgatgcg acgggacgac gttcacgccg ttcgactagg
     2581 cgccgcgcaa catctcgagc gtcgggtgcg cggttctcgg cgctgcgcgg gaggctggtc
     2641 tcggggcaac gaccatttcg cgttctccgt cctccgaggc gtgtttgtcc ccttactcgt
     2701 cttcagggtg ctttagccac ttggggttaa cttttagtta tatccgtggc aatccctcga
     2761 cagcgcgacg gcaagcgtgg tcgcgtgggt cgtgcaccag ctggagtggg gaccttcgtc
     2821 accaaggact gccggcgaac acatgagccg gcaatcgggt cctcgtcgtg gcaaccgccg
     2881 accaccagct gaggagacca tcgtgatcaa cgagagaaag catcgatcga agaggagcgc
     2941 gctcgccgtc gtcgtcgcgg ccgtctgcgc gacgaccctg ctgccgccga cggccgcact
     3001 tgcccaggag acgtcgaggg agagcgcgcc gaccgagcgc gcactcatcg caggcgtcac
     3061 cacctgcacg agggggctgt gtgtgttcac cgcaaccaag tcggccacgc aggccctcaa
     3121 cgcctggctg cccgttgccc agccggccgt ccacgcgttg aagggcatct gtccgctgct
     3181 cggtttctcc gtcgtcggag gcgccatctg cggagccatc gtcttcttcg tcggacgcgt
     3241 gtactccaac gcgaggaacg tgatcagggc tgccgtgacg aacggtcagt gcttcttcat
     3301 caagtacgtg aagtggggct ggatcccgta cgccatgggc cggacctctg cgaactgcgc
     3361 gtagacggac gccgtgcgtg cggaccgcca cgggcccgca cgcaccggct tcgtctcgga
     3421 aggaggaacc gatgggcgaa agcccgagga agaggaagag gaaggtgctc gttcgccgga
     3481 acgtcggcgt tgtggccgcg atgttggccc tgtcgctggg gctggcgccg caggcttccg
     3541 cggcaacgct ctacttcggt ggcggaaccg caacccatac gtccaccttt acggggtctt
     3601 gggtcgcgtc tgcagtcacg gtccaggtgg cggtcaccga cgcgacggat gacgcgtact
     3661 gcgtgcacgc tcggatcacg ccggtgacga cgttcatgcc cttcgcgggg accacgctac
     3721 ggctgcccga cgtgtgcggg cgcggggcgc atcgccgctt cacggtgagg atcgctcgga
     3781 agttcccggc cgtcctgcag tcagtcgtcg tgaggctgtg caggagcgtg ccgagcggtc
     3841 cggtcccgtg cgcggcgagc tacgtcgaca acccgctgac ctgacgcggc ggaaccgcgc
     3901 gcacgatcca acgcggccgg ccctcccggg ggccggccgt tgcgcgtcgg acgctcggcc
     3961 ggctcacctt gactcgaact tgacagctgt taccagcgct ctatataccg gagcaaaccg
     4021 ggaagcggag aggcaggcgt gacctccagc gggcacctca gcgaagacga cgttgcccgt
     4081 tgcgaacccg ggtcggtatc cgttcgccgc gcgtttcccg gcgggtcccc gcgtaagctg
     4141 cagcctcgcg acgtcgaaga cctgatcctg cggtcccgcc cgccggtacg gggccacccg
     4201 gcattgcgcg aacgcgtgcc cccggccacc acgctgcagc ccgtcgagct gaacgccgac
     4261 gagctccgga gcctgctcgt gaaatccctt tcggcgcgcg gcccccaggt ggtgtgggtg
     4321 gacgccggtg acgaggtggt ggtcgacctc gacgcgaccc gcgtcgcgct cacgcccggc
     4381 atggtcttgg tcggcctgtc actgaccacc gacgcgcacg cgcacgccac cgtgtcggtc
     4441 ccggttgcga tcggcacgcc cgagcggatc accgggctcc acgcggtcac cccgcgggtg
     4501 ccggaaggcc ctgtcgacct cgtcacccgc tggggccgat ccgtcgtcgc cgcggtctgg
     4561 ggcgcgctcg tggacgtcgt cgaggcaatg gccggagccg taggcgaaga cctcgacggg
     4621 gcgccgctcg cgccggccgc catcgtcacc gacgggaaga ccttcggtgt ggtccctcag
     4681 gcccgtccaa cccgagagca agcgatgacg actccggaga aggacgacgg ccgcaagccg
     4741 aagggtcgcc ccaccagacc cgagccgcgc gacgcggatc cctaccgccc gaaggggcgc
     4801 tgatggtcca gcggccgtct gagctcgagg acctcggcat cgcactgggg ctgatcgagg
     4861 gcagcggcag ctcggcgacg ctcaacaccg aattcttcac ggaccctcga ggggtcctcg
     4921 gcggcacgct cgccgacccg gaccggcgtg ccgcgctcgt cgacctgttc acggacctcc
     4981 tcggaaccga gggcacgccc catgggttcg acgccggcac ctcggccgac gagacctggc
     5041 tacccctggt ggaggggggg ccgggcggcc tctacgtttt gctcacgcct cacccccctc
     5101 agacgaccga cggcgtccgc gtctccgtcg ccgggcgcgt ccaacacgtc gacaacggag
     5161 tggacgcctc cgccagcatc gtgctgccac tgatctgctt cgacaacggc ggcctggtcc
     5221 cgctggtcgg cacggccgac ggaccggtgt cgatccagtg tcgtgtcggc ggcgaggacg
     5281 gcccgctgat cgacgacgac ggcctcgtgc ttcacgccgt gtcggtgcgc gccacgattg
     5341 caacgcaggc cctggccacg ccgccggcca ccctccaggt cctcgtcgag ggccttcgct
     5401 tcggcgcgga cgcgacgccg ctggacgtgg acctggccga cctggccggc agcgtcggcg
     5461 ggccggccgc gcacctggtc ctcgccgcgc tgcgtgccca agcgtcgacg tcgcccgagc
     5521 tcgcccaact gttcgatctg ctcgggctcg gtggcgccgc ggggatccct tccctggacc
     5581 tcggcgcgct ggcctccgac ccccacggca cgctgacggc gtggctccgt tcggtggtcc
     5641 tcggcacggc gtcccgggcc gcgtggttgt cgaagctcgc cggcctgctg ggcggctcgg
     5701 tgccgtcgcc cgacgagctc tgcttcgtcg acaccacggc gacgaaagtc tgcgtgaccg
     5761 tcgccgtcga tggtgacccc acgaccgggg cgccgcgcgt tcggatcggc ctccttgcgc
     5821 gtcacgaggt cgcgggaatc gcgaccggcc acctgagggc gaccgtgaac gccgtcgacg
     5881 tcgtgttgac ccagccgccc ggcgtcgtcg tctttccgag cgcccgcgcc gtcctgcgac
     5941 tcgagggcac cgccggcccc ctggtccacg gcccggccgc gaacaaccgg aggatcgagg
     6001 tcgggtcgct cgacgcgggc atcgagctca ggccggacgg ctcgatcgtg ccggtgttga
     6061 cggccaacga cgcgatcgtc gacctcgtcg gcttcccgac gctggacctg accgacgccg
     6121 acgccgtgat cggcgccgcc gaggacgtag cggagacggc gctgaacgat ctggtcggtg
     6181 acttggccgg cctcttgaac ggcagcccac gcgtgcgcgc cctcgcctcg ctcctcgggc
     6241 tcgtcgctcc acccggcccg gcctgggccc cggcactggt caacccggcg agcctgctgg
     6301 cggacccgtt gcgggagatc tgctgctacc acagccgggt cctcgcagag gacgggggct
     6361 ggggacgcct gctgcagcag ctcgtcgtcc tcgtgcagcc gggcgacgac ccgccgccgg
     6421 ccctgaccgg cgccggcact gccgcggagc cgtggcgcgc gccgttcgcc gtggacggga
     6481 ccaagcgggt cgatctcttc gcgctcggcc gccctcccga cggcgaccat ccgcaactag
     6541 tcctcggcgt agcagtccgg ggcgccaccg acatcggcga cggcaaagag ctcgggttga
     6601 tcgtccgcag cgaggtcctc gccgcgaccc tgccgcgcgc ggcggactgc gtcggtgagg
     6661 cctcggtcac gatgatgccc ctgcacgagg tggtcctccg gctgggcgac gacctccacc
     6721 tcgacgccgg cgcggtcgag gtgctcgccg accgcgtcga ggtaggcgtg cgctggcgac
     6781 ccggcgacga cctcgaaccg atcgtgcgcg tgagcgaccc gcgcgtgtcc accggaggca
     6841 gcttcgtcga actgcccgac atcgacggcg acatcgacct cgacgcgctg ggtaccgccg
     6901 gctggcagct gttcggcgac ctgctcggcg cgtggttctc gggcgggggc cggtcgtggt
     6961 ggcggctgag cgctctcgtc gggctgctgc cgggcgtgcc cgacgcgccg cagatgctcc
     7021 cgatcggcga cctcgcgctc ccgcgtgcct gggacggcct gctccatctc gggagaggcc
     7081 acgtgaccgg caacgggacc tacgacgacc cctgggagat ctcgaccggc gccgcagggc
     7141 tgcccaaccc cgtgctgtgg ctcgacccgg acgggccccc ggacctgcgg cttcctcgcg
     7201 agctgtttcc tcccgccctg agcgacgccc tcgataacgc caccaccccg ctcgaccccg
     7261 agcggctggc gacgctcctc ggcagggccg tcacgttgtc ccccaccctg gccgacgtgg
     7321 tcgacgagga cctcgccgcg gcactgcggt tcctgcgcga ccagttggcc ggcacggacg
     7381 ggttggtagc ggctgcggca caggaggtta cgggttggaa ccgcatagac ctcgcgtcgg
     7441 cgttgcacct cggcgagccc gccgtgctcg acctcgacgc gcaggtgccg gggctgccgg
     7501 gcgggagccc ccgcgtcttc gtctccgcgc cgctcccgtg ggcagcggcc tggcggggcc
     7561 aagatcctgc acgcgtgatc gacctgcgtg aggctgggcg cgagcccgcg acgttcgacc
     7621 tcgaccacgt caccgcgggc gggagctggt tcgtcctgct tcccccacgc gccgacgccg
     7681 tcactgcgac gatcgccgac ggcctcgagg gactgcgcgc acgactgcac gccgccgtcg
     7741 cggccgtgcg cgacgcggcc ggcgcgccgg tcgtgctcgt cgcacatgcc acctcgggtc
     7801 acccggcgcg gctggtcgcg gccgatgccg gcctcgtctc cgacatcgtc acggtgggca
     7861 cgccacactc gacgtccgcc atcgggtggg tcgcgcagga cgagggccgg cgggcgctgc
     7921 gcgcgctcca ccgcctccgg gcgcgagcgc gtggcgcggg agccgacctc ggccccctcg
     7981 cggacctgtt ggacgggccg ctcgcggcgc tcgagggcat cgtcgcggag gtcgacggca
     8041 ccgacgtcct gcgacgccac gtcccgttcc ccttcgcgga ttacgtgacg ccggcgtcgt
     8101 ggcccgcgct ggcggcaggc gtcaccgggc acgccgtggt cgcgacgatc gtgcccgcgg
     8161 aagtcgacgg cgcgctgacc acgttggtcg gccgtgcgat cgagggcctc gcgacgttac
     8221 cggccggccg tacggcgccc actcacttgg gtatcgggtt gcggggtctg cgacgtgacg
     8281 gcagcagcct cgccggcgag gtccgcgtcg acacccgcgc tcgcgtcgac gtccacctgg
     8341 tcgagatcac gcccggcgat caccgggccg tgccgcgcct cggcatccac gcagacatct
     8401 cgcgcaacgg cggctggctg gtccgtccgg cggacgtctc gatgggccgc cggcaggacc
     8461 cgcgtgtggg tcgcgcggag atcgacctcg ccctcgatct cggcgacccg tcccccgccg
     8521 tgtccgaccc ggccccgcgt ctgtcggcac ggatcgtcct ccacgacgtc accgtgctcg
     8581 gacgcttcca cgagcgctgg gtcgtcgacg acaccctgct ggccgcgctg gcggacggaa
     8641 ccagtggcgg caccggcacc ttgccggagg tccggatagt gctcggcatg gtggcgaccg
     8701 cactcgggaa cgtcggaggc accggtccgg tccgcgacct cgccggcctg ctcgtcgccc
     8761 tgcaggtcgg gacgatcgac ggcaacgcgt tcgacttcac gaccgagggg ctcgagcggc
     8821 tgctgcgcga gccggaccag gtcgtcgccg acctgctggc cgacgccgat cgggccgacg
     8881 acttcctgtc ggcgctggcc gcgtgcacgg gcggcaccgc gacgggggac acgacgatgc
     8941 tgggcacggg cgggctaccg ttcggcatgg tcggaccact cggttcgccg tcgctcaggc
     9001 tccgcacgtc gggccccgtc gaggtcgcca agcaggtgac gatcgacgcc gacgtcacca
     9061 tcgacgcgac caggcgcgcc cacgggtcgg tcaccgtgac cagtcccgtc gacgtcggag
     9121 gcctcggcaa cccgcgcttc gtcgtcggga tcgacacggc gacggcggac ctcgtgaccg
     9181 tcgcgttcga gctcgacggc gacggcggcg gggcggaccg ggtggcgctg ttccccgacc
     9241 cggacttggt cgccctcgcc cgcttggcgg gtcaggtgat cgcggccgag gtcgcccggc
     9301 aggcgttgac gtgggcacac gcgcagcagc ccgcgctcgt cggagccgtc ttgggcgccg
     9361 tcgggctgct caacccgccc gccgcgcccg aacccgtccg gtcgctgctc ggcctgatac
     9421 gcgacccggt ggcgtggctc acctccgacg tggcgctggg ggccggcggc actgcgctca
     9481 ccccggctag cgtcgtagcc gcggtggacg ccgtcgccgc cctcctcggc caggtgccgg
     9541 cgggcggggt gttgaccctc cccggcggaa tcacgctgac cgcctcgccc gacggggacc
     9601 gcaccca
//
LOCUS       DASQDO010000549         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_608247, whole genome shotgun sequence.
ACCESSION   DASQDO010000549 DASQDO010000000
VERSION     DASQDO010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608247"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VEV43_15125"
     CDS             <1..1350
                     /locus_tag="VEV43_15125"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HYP24898.1"
                     /translation="AIAAVLFGGVALDTWGPKVSADPAVRAASAPYEARAVFCPPALG
                     KPASRMTVTTAARGDEPVTVGFEPASDERVELTPGTVLESKPPTARALDVVGYGAPVD
                     ASVVTSIDDPISGVGAAGCASEASTRWFFPEGNSTVTHDERLVIYNPFPDEAVVNVSL
                     LTPGGEKSKAGLADRAVPSESSVTLALNDFVLEQKVLGAVVSATRGRVVAWRLTIARP
                     DQLPSGVQFTLGATRTAETWYFPEGAVGTGFRERLSVLNPGTDEAIVNVTLVNDARAV
                     PAAGSTEVQVPPRSSMEIVLDEAALAGERGGAGAIVNSANGVPIVVERTVFYTTQEVD
                     GTASEIGSPSPSLRWLLGPATSRPDTDSVVLLSASADRARVSLTLLRPDGRAVEPERL
                     QDLPLAAGTRLRIPLSEITAGEAYTVVVDSTAPVVAERFSYSGGAGDVASLMGTPLD"
     gene            complement(1358..>1655)
                     /locus_tag="VEV43_15130"
     CDS             complement(1358..>1655)
                     /locus_tag="VEV43_15130"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYP24899.1"
                     /translation="LDETSPLAEILGSGAAPVNSHHHQGLDRVGEGLEEIAWAEDGVL
                     EGVYAPDMSWVVGVQWHPEAMAPVDPNQLAIFENFVSAAEQHTQPRRSAAELSA"
BASE COUNT          217 a          588 c          621 g          229 t
ORIGIN      
        1 gccatagccg ccgtcctgtt cggtggcgtg gccctcgaca cgtgggggcc gaaggtgtcg
       61 gccgacccgg cggtgcgcgc ggcgagcgcg ccctacgagg cccgggcggt gttctgcccg
      121 cctgcgctcg gcaagcccgc gagccgaatg accgtgacga ccgcggcgcg cggcgacgag
      181 cccgtcacgg tcggcttcga gcccgcttcc gacgagcgcg tcgagctgac accgggcacg
      241 gtcctcgaga gcaagccccc gacggcgcgg gctctggacg tggtcggtta cggggccccg
      301 gtcgacgcga gcgtcgtcac gagcatcgac gaccccatct ccggcgtcgg cgccgccggc
      361 tgcgcgtccg aggcgtcgac gcgctggttc ttccccgagg gcaacagcac ggtcacgcac
      421 gacgagcgcc tcgtgatcta caacccgttc cccgacgagg ccgtcgtcaa cgtgtcgctg
      481 ctcacgcccg gcggcgagaa gagcaaggcc ggtctcgccg acagggcggt gccgtcggag
      541 agctccgtga cgctcgcgct gaacgacttc gtcctcgagc agaaggtcct cggcgcggtc
      601 gtcagcgcga cgagaggacg ggtagtcgcc tggcggctga cgatcgcgcg gcccgaccag
      661 ctcccttccg gcgtccagtt cacgctcggc gccacgcgca ccgccgagac ctggtacttc
      721 cccgaggggg ccgtcggcac ggggttccgc gagcggctgt cggtcctgaa ccccgggacc
      781 gacgaggcga tcgtgaacgt gacgctcgtg aacgacgccc gggcggtccc cgccgccggg
      841 agcaccgagg tccaggtgcc gccgcggtcg tccatggaga tcgtcctcga cgaggccgcg
      901 ctcgcgggcg agcggggcgg cgcgggcgct atcgtcaact cggccaacgg ggtcccgatc
      961 gtggtcgagc ggacggtctt ctacacgacg caggaggtcg acgggacggc gtccgagatc
     1021 gggtcgccgt cgccgtcgct gcgctggctg ctgggcccgg ccacctcacg ccccgacacg
     1081 gattcggtgg tgctgctgag cgcgagcgcc gaccgggcgc gcgtctcgct cacgctgctg
     1141 cggccggacg ggagggccgt cgagccggag aggctccagg acctgcccct ggcggcgggg
     1201 acacgcctac ggatcccgtt gagcgagatc acggccggcg aggcgtacac ggtggtggtc
     1261 gactcgaccg cgccggtggt cgcggagcgg ttctcctatt cggggggcgc gggcgacgtg
     1321 gcgtcgctca tggggacccc gctcgactag agaggagtta ggcggagagc tcggccgccg
     1381 aacgcctcgg ctgcgtgtgc tgctcggctg cggagacgaa gttctcgaag atcgcgagct
     1441 ggttcgggtc cacgggcgcc atcgcctcgg ggtgccactg gaccccgacg acccagctca
     1501 tgtcgggggc gtacacgccc tcgagcacgc cgtcctcggc ccacgcgatc tcctcgaggc
     1561 cttcgccgac tcggtcgagg ccctggtggt gatgcgagtt cacgggggcc gcgcccgagc
     1621 cgaggatctc cgccagcggg ctcgtctcgt cgagc
//
LOCUS       DASQDO010000550         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_546390, whole genome shotgun sequence.
ACCESSION   DASQDO010000550 DASQDO010000000
VERSION     DASQDO010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546390"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VEV43_15135"
     CDS             <1..369
                     /locus_tag="VEV43_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24900.1"
                     /translation="TMRPHRWFGNRVLTVAVRLLSRLPVSDGQSGYRALSPAAAADAE
                     VIHDFNYAQVLTLDLLGKGFRYAEVPIGYGFRTTGKTFVKLGRYLRAVVPAVHRELNE
                     ISPPPRALERTPARDASALR"
     gene            complement(530..1699)
                     /locus_tag="VEV43_15140"
     CDS             complement(530..1699)
                     /locus_tag="VEV43_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYP24901.1"
                     /translation="MSARIAGETDLLVLGAGPAGVGAAYRAARSGRKVVVVERAPGPG
                     GAARSIRVAGQSVDLGSHRLHPSIRPEILGELRGLLGDELQLRPRHGRIRLGGRWVRF
                     PLSPPDAVKNLPPSFLARAARDAAVRPFRREREDTFAGVLRAGLGPAICDAFYFPYAR
                     KIWGVEPERLSAEQARRRVGARSPARIVAKVARRKGTSFWYPAGGYGRISEALAEGAA
                     GAGAELRYGRTVERLALSDDRVVATLDDGAEVEAARVWSTIPVTALASMCEPRPPEGV
                     VAAAGSLRFRAMVLVYLVVAADRYSEYDAHYLPEEWTPVTRISEPKNYRDGDDRPGRT
                     VLCAEIPCDPGSATWDAGDDDLGALVTEALRGSDLPAPAVEAVVTHRLSHAYPVY"
     gene            complement(1696..2703)
                     /locus_tag="VEV43_15145"
     CDS             complement(1696..2703)
                     /locus_tag="VEV43_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYP24902.1"
                     /translation="MAVSGVTKAFGTTPVLNGVDLDVPAGTVVALLGPSGCGKTTLLR
                     AIAGLETPDGGTVSIGGRLLAGPGVHVPPEKRRVGMVFQDWALFPHLSVAGNVGFGLP
                     KAERSSRRVDETLEMVGLAGLAARMPSTLSGGQQQRVALARALANRPSVILLDEPFSN
                     LDASLRHQIRLEVQRLLSDLRVTSLFVTHQQEEAFVVGQEVAVMLDGVVVQQGAPATL
                     YEAPATIEVARFVGDANFLRGTAAADKAETCIGPVPLDRDHSGEVDVMVRPEQVGIYA
                     GDEGTVEHVEFYGHDSIYLVALDGGTTVRSRVLTTPEFRPGDRVALGFTGRPAIAFAA
                     V"
     gene            2909..>3507
                     /locus_tag="VEV43_15150"
     CDS             2909..>3507
                     /locus_tag="VEV43_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HYP24903.1"
                     /translation="MPLYDPVSGERDACGIGFVADSSGRASRAIVQSALDALCRVKHR
                     GAVAADELTGDGAGLLLPIPPNVAPAGRGVAMVFFDPAEVAAGRDAVEAACAAEGLTV
                     ESWREVPVEPAALGEGARATAPAIRQAILAPHDDGELRAYRARRRAEASGVPLFVASC
                     SYRTVTYKALCAADQLARFYPDLAAPGCSAWFALFHQRYS"
BASE COUNT          479 a         1324 c         1238 g          466 t
ORIGIN      
        1 accatgcgcc cgcaccgctg gttcggaaat cgcgtgctga ccgtggctgt ccgcctgctc
       61 agtcgcctcc cggtcagcga tggccagagc gggtaccggg cgctctcccc cgccgcagcg
      121 gccgatgccg aggtgatcca cgacttcaac tacgcccagg tgctgaccct cgacctgctg
      181 ggcaagggct tccgctacgc cgaggtcccg atcggctacg gcttccgcac gacgggcaag
      241 acgttcgtga agctcgggcg ctacctccgc gccgtagtac ccgccgtcca ccgagagctc
      301 aatgagatca gtcctccacc acgtgctctc gaaaggactc ccgcgcgcga cgccagcgcg
      361 cttcgttgaa cccgccgccg ggttccaggc agtccgcggc gtcccacgcc atcgccagcg
      421 cgtggtgcgc gttgtcgtgc acgaacaggc cctgacggcc gaacgtcagt aggcgcggct
      481 gcgcgtcgag ccacgcgtgc agctcgccga gcggctcttc gaacccccgt tagtacaccg
      541 ggtacgcgtg cgacaagcgg tgcgtcacga ccgcttcgac ggcgggggcc ggcaggtccg
      601 agccccgcag cgcctccgtc acgagcgcgc cgaggtcgtc gtcgcccgcg tcccacgtcg
      661 ccgatcccgg gtcgcacggg atctcggcgc acaggacggt ccggcccggc cggtcgtcgc
      721 cgtcgcggta gttcttcggc tccgagatgc gcgtgaccgg ggtccactcc tcgggcaggt
      781 agtgcgcgtc gtactccgag taccggtccg cggcgacgac gaggtagacg aggaccatcg
      841 cccggaagcg caacgacccc gccgccgcga cgactccctc cgggggccgc ggttcgcaca
      901 tgctcgccag cgccgtcacc gggatcgtcg accagacacg cgcggcttcg acctcggcgc
      961 cgtcgtcgag agtcgcgacg acacggtcgt cactcagcgc gagacgctcg accgtccgcc
     1021 cgtagcgcag ctcggccccc gcccccgcgg ccccctctgc gagcgcctcg gagatgcgcc
     1081 cgtaaccgcc cgccgggtac cagaaggacg tccccttgcg ccgagcgacc ttggcgacga
     1141 tacgcgcggg cgaacgtgcc ccgacgcgcc ggcgcgcctg ctccgccgac aggcgttcgg
     1201 gttcgacgcc ccagatcttg cgggcgtacg ggaagtagaa cgcgtcgcag atcgcggggc
     1261 cgaggccggc gcgcagcacc ccggcgaacg tgtcctcccg ctcgcgccgg aacggacgaa
     1321 cggccgcgtc gcgcgccgcc cgggccagga acgacggggg caggttcttg accgcgtcgg
     1381 gcggcgagag tgggaagcgc acccatcgac cccccagccg tatgcgcccg tggcgcggcc
     1441 tcagctggag ctcgtcaccc agcaacccgc gcagctcgcc caggatctcc gggcggatcg
     1501 agggatgcag gcggtggctg ccgaggtcga cgctctggcc cgcgacgcgg atgctgcgcg
     1561 ccgctcctcc gggccccggc gcgcgctcga cgacgacgac cttgcgtccg gagcgcgccg
     1621 cgcggtacgc ggcccccact cccgcgggcc ccgcccccag tacgaggagg tctgtctccc
     1681 cggcgatccg cgcgctcaga ccgcggcgaa ggcgatcgca ggacggccgg tgaagccgag
     1741 cgcgacgcgg tcgccggggc ggaactccgg cgtcgtcagc acccgggagc gaacggtcgt
     1801 gccgccgtcc agcgcgacga ggtagatcga gtcgtggccg tagaactcga cgtgctcgac
     1861 ggtcccctcg tcgcccgcgt agatgcccac ctgctcgggc cggaccatca cgtcgacctc
     1921 gccggaatgg tcgcggtcga gcgggacggg gccgatgcaa gtctcggcct tgtcggcggc
     1981 agcggtccct ctcaggaagt tcgcgtcgcc gacgaagcgc gcgacctcga tcgtcgcggg
     2041 agcttcgtag agcgtcgccg gcgcgccctg ctgcaccacg acgccgtcga gcatcaccgc
     2101 gacctcctgc ccgacgacga acgcctcctc ctgctggtgc gtgacgaaca gcgacgtcac
     2161 gcggaggtcg ctcaacagcc gctggacctc gagccggatc tggtgacgca gcgacgcgtc
     2221 gaggttcgag aagggctcgt cgagcaggat gaccgagggg cggttcgcga gggcccgtgc
     2281 gagcgcgacg cgctgctgct ggcccccgga gagcgtcgac ggcatgcggg cggcgagccc
     2341 ggcgagcccg accatctcga gcgtctcgtc cacgcgcctc gagctccgct ccgccttggg
     2401 aagcccgaag cccacgttgc ccgcgacgct gaggtgcggg aacagcgccc agtcctggaa
     2461 gaccatgccg acgcggcgct tctcgggcgg gacgtgcacg ccggggccgg cgagcagcct
     2521 gccgccgatc gaaacggtcc cgccgtcggg cgtctcgagc ccggctatgg cgcgcaggag
     2581 cgtggtcttg ccgcaaccgc tggggccgag gagcgctacg acggtcccgg ccggcacgtc
     2641 gaggtcgacg ccgttcagca cgggggtcgt cccgaacgcc ttcgtcacgc cggacacggc
     2701 cacgcccgcg gacgtctgga acgacgttgt tgggggggcc gtaggcgcga ggttaggcat
     2761 acctaagaaa gtacaccgcc gcttggtccc ctcgccacgg aggtcgttga cactgcccca
     2821 gggggccggt acgttgcgga tcgacacaga ccactgagca acggcgctcc tttcccgggg
     2881 cgcctttttt gttgcccgga ggtgagccat gccgctgtac gacccggtct ccggcgagcg
     2941 cgacgcgtgc ggtatcggct tcgtcgccga ctcgtccggg cgcgcctcca gggcgatcgt
     3001 ccaatctgcc ctcgacgcgc tctgccgcgt caagcaccgg ggggccgtgg ccgccgacga
     3061 gctcaccggc gacggtgccg gcctgctcct cccgatcccc ccgaacgtcg cccccgcggg
     3121 ccgcggcgta gcgatggtct tcttcgaccc cgccgaggtg gccgcgggcc gggacgccgt
     3181 cgaggccgcc tgcgcggccg agggcctgac ggtcgagtcg tggcgcgagg taccggtcga
     3241 gccggccgcg ctcggcgagg gcgcgcgggc gaccgccccg gcgatccggc aggcgatcct
     3301 cgccccccac gacgacggcg agctccgcgc ctaccgggcg cgccggcgag ccgaggcgag
     3361 cggggtgccg ctgttcgtcg cgtcgtgctc gtaccggacc gtcacgtaca aagcgctgtg
     3421 cgccgccgac cagctggcgc gcttctaccc cgacctcgcg gcccccggct gcagcgcgtg
     3481 gttcgcgctg ttccatcagc ggtactc
//
LOCUS       DASQDO010000551        10070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_363336, whole genome shotgun sequence.
ACCESSION   DASQDO010000551 DASQDO010000000
VERSION     DASQDO010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363336"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5011
                     /locus_tag="VEV43_15155"
     CDS             <1..5011
                     /locus_tag="VEV43_15155"
                     /inference="COORDINATES: protein motif:HMM:NF042646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6603 domain-containing protein"
                     /protein_id="HYP24904.1"
                     /translation="SVHVGFDASGPTLSASITPEGGSALVLPILPPATWGDLASLGAG
                     GVQQVLPMLLDAVAAEVPAVGTLGDAFGLRTNGAFDGAKLVALAVDPAAALNGAQVAT
                     ALQTLLTAAGVPATVSGSTLSFAPVTGLDVDVTTGPTPRLCVSVDPIALRPELSFGGE
                     ACVDSAGTFTSQLLLEVTDPNLLDTDPIVLLPFVRIQASSAGPNVLLAGAWNGVPRQA
                     GNEAVAARVVFGSGTVDVVCLIGDYTPAPDQNACIALWAGGLVLPLVADLVLAAQAVK
                     DLLNEALLGETALIGQALHDAGILVRTGDDYAIVGDLLANMETFGRRLVVLGAKLLEA
                     TENQILGDALGPLEITPVVEEDGGRRWFGLTLALDEAMTLADGSDVRLELDTSRGTFG
                     GSEPRGLSLFLASHPTGDAAAIRVAPRIAAREIGLRLTGAAGGPLLSDPIGIGEAGLF
                     GTYEGGCTFAGDEVDGWETDGGGVRIAVRRLAVPLTGSDDGGNPVAQKILSQATETSQ
                     AGDDEPLAPSVDAELEVGTDGVQLHLGLGDPPWWLPVQRSFGPLYVGQVGLDIEPVDA
                     PNEIAVLFDGGIELAGLSIGVDDLAIIVTLDTRDVAIGLAGLAIGYDSNGLKLAGGLR
                     RHGPDYLGMLQLQFGEFGLTAIGGLGEHPHDGGTYTALFVFGVLTAPLGGPPPFFVTA
                     IGAGFGVNRRLVLPETLDALPQFPLVAAMSPGSDLAQDPMGALDALGQTFPPHRGTFW
                     FAAGVRFTSFVLVESIAVLSVEVGDGLEVALTGISRTTLPRPELPMASVELALRARFS
                     TREGVLAIQAELTENSWLLSSSCRLTGGFAFVVWFRTGQFVLSLGGYHPRFAKPDDFP
                     DLDRLGYHWQVSSVISIKGGQYFALTSSAVMAGGTFEASYRTSWSWASFEMGWNVLIM
                     WDPFAYDLQVYVRISAGVRIRVCVPLLGCATAGFSFSIGANVHVLGPELRGTAVLDLD
                     VTTITVAFGASGDSNADEGLPWDEFRRKYLTDGDTSNGLEAALGNGRYVEAGAPASEA
                     AGAADGPWLLLPEFDVTVTTKTASNRFGSDPVPGVDQIDLGPMRRANVVTDLEVKLFP
                     VADGDADVTGNEGVTLTPRLGQQPEAIWRTHAKSSDAPPAAELVTAVTGATLVVRSKI
                     LDRTGEIPVDQVDVGVKVHPLPLRQQVRWRTGLAPWVTGAAALVEWAGSRSVSAAVDL
                     LQTRGTLAAVRERDRWRVAAETTKVALSGAAPALEGLSARASALVTHDGQRAARPVSL
                     AGRAAPPRVRSLGRGTTPPEPTRVEVGVADPPAVAELPAAKLRPRLDAVLHEPTSTAQ
                     IAVATRALEVGAGLPRMAAPSLEAVTAEAITSVGAHLHLTRPGTPAARAVGGRAATAM
                     AGDVHEWRTADVKLSSHAAAALQEIDEGLDGDGVALRAGDVHVWRIPDSRRDQGTERP
                     SLHVESEVPVRVVAIDRGGRPLADELLTSGDVAVPAGADRISLAVTGEPGGVAGWHTG
                     TRLARISPTTLLCRRGTVRSEAAASRRSRRETATGLVPAMDVVPTRGTVETTLPATTR
                     TVVLGLSPTTDDVDPEAFAMTLERATRDESDPVVLRVGDRLYGLFGVTPDEDAGTLVA
                     AVRTDARWELAGVLGTEDDPRTAADWIRSGGLTRLAGLVTTPAGSARVRWREPEYEMA
                     R"
     gene            5008..6873
                     /locus_tag="VEV43_15160"
     CDS             5008..6873
                     /locus_tag="VEV43_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24905.1"
                     /translation="MSGPPPPGHIRLYTAIRPALPAGTYRLETSQRITYDSQTQSLPS
                     AGQTIELVAPRFRMSGSELLSTFPPAFAQGPFSNRFAQVALKRRTLPWERDPGAGADG
                     RKTPWLMLVVLAKGEGELEPNTPLSSSDLAELRTAVGLSQAEAAEAGSCDVLRVTGRI
                     VRKCFPSRTDLPYLAHVREVNLLDTETAAGDDDGFVAIVTSNRVLRGRQSYTAYLVSV
                     EGQLGALQGPRETTDEVGPAATTPGDPGGGGGRDLLMIKEHPVANLFASDAVKSAARD
                     AGRSDVKRVTEHQRERLQGWDDPVDAVGGAGEHLVSHELFAHAKDRGMIVELGHIEVL
                     AAADTEPGDDRVHRFPVLAHWTFQTIGNGDFETVMRGLDVGLLGTVDADAEDAPTVVP
                     TGHVLIDRRTRKGEVSAGWYRGPLVPHELEDRDDVPLHAAEQALRIGADGIEDLSYAS
                     AFELGRLMALADPRFVAALVAWRRRGATRRWRRLSSELFETGLLPSLPLDKLEGLGID
                     VDLVDRLIAFEEPNDPFGPLGPLVDPVPWRGLYDPREDVAVLRTIGIERAQIEDALGI
                     GVASGFGSGEELVRADVDPMDFDTLAERGGGEMADELERHHQVDLRGIESLGGES"
     gene            6870..8018
                     /locus_tag="VEV43_15165"
     CDS             6870..8018
                     /locus_tag="VEV43_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24906.1"
                     /translation="MTTHWFKSQGVVPGLRKAWAPEDADDDVAAAPAEVLDWLARLRL
                     LHGVPFAYLVVDDDLLPPESIRFFWMDRDWTDAAVEGALSAAGAHSLARTLAATEDAA
                     LRQRLDQRERNITPHDPRLAQAERPPGGGPTRTDDHHAGHLPYDTLSGPAGTVTGFVL
                     RSKAVSGWPELHVRALDDRGNTMTLLRVERLAPAVLLVLIDGCPNEVQIEEPRRGIQF
                     GVDLTPNTVPAGEEHPDEHPDGGRQVALRDPENGLTVLLKAGARVPNPPAGSPDPSGS
                     KRTQVAVPFREKTTGVVDMAALAAHFAGAVDANPGAGLQFTSDDDEEAWPHMVSTAEL
                     TTQLLQFPYQQRFHGDGHDPDGGRVGAVLAVTTPVEIVEMFTAFGHLE"
     gene            8032..>10070
                     /locus_tag="VEV43_15170"
     CDS             8032..>10070
                     /locus_tag="VEV43_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24907.1"
                     /translation="MRIDPRDPAFDPKVVWRRNLDIAVDPAALVFDRDVRPFDGDGPA
                     LLVPVDVQALSVPEDGAVDHADTSVRLVDAQELGDLAEATAAPFTEGVGRSAGIHLHW
                     AMPDALTRGTTSESGVLSQPSLPDRWLVVRLGPGTERPWAGWVIESDYGRATPLSQWE
                     GEPGDPDGDPPAGWIPPESLSAASGGDPAWAATFDNVVNRFAFHDPLTDVDGDLFTYA
                     VVGWYGDDSLDPLDDVRSMGSFDHVMQSLGWTVSPEAHERARLEGLIRSNAFATAGAE
                     GRAFRKVANRRELAVGAAFDDLTVDALDSVWPRRCVFHGAVFGVRRDGVGADHRPTPH
                     DLSAALGATATEALARLIGDRHPQGGAVEERALAAFAYGIADRLGEPDGPALVEEQMH
                     GRAFESRPGPPLHDWIRRTDRLKDVRPPKIRPAREDVLGGMLDEAAITRKDVAWKARF
                     DFLPDADKDRIHEVGGVDVGKRLQAAVREIDRIEKVTRAGPRYFQPQDPVVAIQGSER
                     HLRHGYDGRWTADETVACRLAGDTQDEWDDLTGAELLASPFVHAALPPEARHLVHEAA
                     LEDPSNVEETIAAATRRDPNRAGEIAARIRAEVVIGRYGRDPERQTDALAKGSSRHGT
                     MSSGIGFNEWDGPPWIPLHLEWMIELDVGDRLGGWELGELDFDPPKSVDADPTKVL"
BASE COUNT         1431 a         3628 c         3627 g         1384 t
ORIGIN      
        1 aagcgtccac gtcgggttcg acgcctcggg accgacactg tccgcttcga tcacgccgga
       61 aggcggcagc gcgctcgtcc tgccgatcct gccaccggcg acttgggggg acctcgcgtc
      121 gctgggggcc ggcggcgtgc agcaagtgct gccgatgctc ctcgacgccg tggccgccga
      181 ggtgcccgcc gtcggcacgc tcggcgacgc gttcggcctg cggacgaacg gcgcgttcga
      241 cggcgcgaag ctcgtcgcgc tggccgtgga tccggcagcg gccctgaacg gagcacaggt
      301 ggcgacggcc ctccagacgc tcctcaccgc ggcgggggtc ccggccaccg tctccgggtc
      361 cacgttgtcc tttgcgccgg tcaccggcct cgacgtcgac gtgacgaccg gaccgacacc
      421 gcggctgtgc gtgagcgtcg atccgatcgc gttgcggccg gagctgagct tcggcggaga
      481 ggcgtgcgtc gactctgccg gcaccttcac gtcgcagctc ctgctggagg tcaccgatcc
      541 gaacctgctc gacaccgatc cgatcgtcct gctcccgttc gtgcggatcc aggcaagcag
      601 cgccggtccc aacgtgctgt tggccggcgc gtggaacggc gttccccggc aggccgggaa
      661 cgaggcggtc gcggcgcggg tcgtgttcgg gagcggcacc gtcgacgtcg tgtgcctgat
      721 cggcgactac acgccggcgc cggaccagaa cgcctgcatc gcgctgtggg ccggcggcct
      781 cgtcctgccc ctggtggccg acctggtgct ggccgcgcag gccgtcaagg acctcctcaa
      841 cgaggccctg ctgggcgaga cggcgctgat cggccaggcg ttgcacgacg cgggcatcct
      901 ggtccgaacc ggcgacgact acgcgatcgt cggcgacctg ctcgccaata tggagacgtt
      961 cgggcgccgg ctcgtcgtgc tgggcgccaa gctcctggaa gccaccgaga atcagattct
     1021 gggcgacgcc ctcggcccgc tggagatcac acccgtcgtc gaggaggacg gcgggcgtcg
     1081 gtggttcggg ctgacgcttg ctctcgacga agccatgacc ctggccgacg gcagcgacgt
     1141 ccgcctcgag ctggacacct ctcgcggcac gttcggcggc agcgagccgc gcggcctcag
     1201 cctgttcctc gcgtcccacc caaccggcga cgccgcggcg atccgcgtcg caccgaggat
     1261 cgccgcgcgc gagatcgggc tgcgactcac gggagccgcc ggcggaccgc tgttgtcgga
     1321 cccgatcgga atcggggaag ccggcctgtt cgggacctac gaaggcgggt gcacgttcgc
     1381 cggcgacgag gtggacggct gggagacgga cggcgggggc gtccggatcg ccgtccggcg
     1441 cctcgcggtc ccgctgacgg ggtcggatga cggcggtaac cccgtcgcgc agaagatcct
     1501 ctcgcaggcc accgagacct cccaggccgg cgacgacgag ccactcgcgc cgagcgtcga
     1561 cgcggagcta gaggtcggaa ccgatggtgt gcagctacat ctcgggttgg gcgacccacc
     1621 gtggtggctg ccggtccagc gttcgttcgg accgctgtac gtcggccagg tcggcctgga
     1681 catcgaaccg gtcgacgccc cgaacgagat cgcggtcctc ttcgacggcg ggatcgagct
     1741 ggcgggcctg tccatcggcg tcgacgatct cgcgatcatc gtcacgctcg acacgcgcga
     1801 cgtcgccata gggctggccg gcctcgcgat cggctacgac agcaacgggc tgaagctcgc
     1861 cggcggcctc cgtaggcatg gcccggacta cctggggatg ctgcagctcc agttcggcga
     1921 gttcggcctt acggccatcg gcgggttggg cgaacacccc cacgacgggg gcacctacac
     1981 ggccctgttc gtgttcggcg tccttaccgc gcccctgggc ggtcccccac cgttcttcgt
     2041 gacggccata ggcgccgggt tcggcgtcaa ccgcaggctc gtcctgccgg agaccctcga
     2101 cgcgctgccc cagttccctc tcgtcgccgc aatgtcgccc ggcagcgacc tggcgcagga
     2161 cccgatgggc gccctcgacg cgctcgggca gaccttccca ccgcaccgcg gcacgttctg
     2221 gttcgcggcg ggcgtgcggt tcaccagctt cgtcctcgtc gagtcgatcg cggtgctgtc
     2281 ggtcgaggtc ggtgacggcc tcgaggtcgc gctgacgggg atctcgagaa cgaccctgcc
     2341 tcggcccgag ctgccgatgg cgtcggtcga gctggcgctc cgcgcacgct tctccacgcg
     2401 cgagggcgtc ctcgcgatcc aggcggagct caccgagaac tcgtggcttc tgtcgagctc
     2461 gtgccggctc accggcggct tcgcgttcgt cgtctggttc cggaccggcc agttcgtgct
     2521 gtcgctcggc ggttaccacc cgcgcttcgc caagcccgac gacttccccg acctcgaccg
     2581 gctcggctac cactggcagg tctcctccgt catctcgatc aaggggggcc agtacttcgc
     2641 gctgacgtcg tccgcggtca tggccggggg caccttcgag gcgagctacc ggacgtcctg
     2701 gtcctgggcg tcgttcgaga tgggctggaa cgtcctgatc atgtgggacc ccttcgccta
     2761 cgacctccag gtctacgtcc gcatctcggc gggagtccgg atcagggtct gcgtgccgct
     2821 gctcggctgc gcgacggccg ggttctcgtt ctcgatcggt gcaaacgtcc acgtgctcgg
     2881 ccccgagctg cgtgggacgg ccgtgctgga cctcgacgtc accacgatca ccgtggcgtt
     2941 cggcgcctcg ggcgacagca acgccgacga aggcctcccg tgggacgagt tccggcgcaa
     3001 gtacctgacc gacggggaca cgtcgaacgg gttggaggcc gccctcggaa acggccggta
     3061 cgtggaggcc ggtgcccctg cgtctgaggc ggccggggcg gccgacgggc cctggctgct
     3121 cctgcccgag ttcgacgtca ccgtcacgac caagaccgcg tccaatcgct tcggttccga
     3181 cccggtcccc ggcgtcgatc agatcgacct cggtcccatg cgccgcgcga acgtcgtcac
     3241 cgacctcgag gtcaagctgt tccccgtcgc cgacggtgac gccgacgtca ccggcaacga
     3301 aggcgtcacg ctgacacctc gcctcggcca gcagcccgag gcgatctggc gcacgcacgc
     3361 gaagtccagc gatgcgccgc ccgccgccga gctcgtcact gccgtgacgg gcgccacgct
     3421 cgtcgtccgc tcgaagatcc tcgatcgcac cggcgagatc ccggtcgacc aggtggacgt
     3481 cggcgtgaag gtccatccgc tgccccttcg ccagcaagtc cggtggcgaa ccgggctggc
     3541 cccctgggtg accggcgcgg ccgcactggt cgagtgggcg ggcagccggt ccgtgtcggc
     3601 ggcggtcgac ctgctccaga cccgggggac cctggccgcg gtacgggagc gcgaccggtg
     3661 gcgagtggcg gccgaaacga cgaaggtggc tctttccggc gccgcgcccg cgctcgaggg
     3721 cctgtcggcg cgagcgagcg cgctcgtcac ccacgacggg cagcgagccg ctcgacccgt
     3781 ctccctcgcc ggccgcgccg cgccgccgcg cgtccggtcc ctggggcgcg ggacgacacc
     3841 cccggaaccg acgcgtgtcg aggtgggggt ggcggatcca ccggcggtcg ccgagcttcc
     3901 cgccgccaaa ctgcgtccgc gactcgacgc cgtcctccac gagcccacca gcaccgcgca
     3961 gatcgccgtc gccacacgcg ctctcgaggt cggagccggc ctgccgcgga tggcggcacc
     4021 ctcgctcgag gcggtcaccg ccgaggcgat cacctccgtc ggcgcgcacc tccacctcac
     4081 ccggcccggc acgccggccg cccgcgccgt cgggggccgg gccgccacgg cgatggccgg
     4141 cgacgtgcac gagtggcgta ccgcggacgt caagttgtcg tcgcatgcgg ccgcggccct
     4201 acaggagatc gacgagggcc tcgacgggga cggggttgcc ctgcgggccg gcgacgtgca
     4261 cgtatggcgg atcccggaca gccgccgcga ccagggcacc gaacgaccga gcttgcacgt
     4321 cgagagcgag gtgcccgtcc gggtggtcgc catcgatcgc ggaggtcgcc ccctggccga
     4381 cgagctgctg acgtcggggg acgtcgccgt ccccgccggc gccgaccgca tctcgctcgc
     4441 cgtcacgggc gagccggggg gcgtggccgg ctggcacacg gggacccggc tggcgcgcat
     4501 ctcgccgacc acgctcctct gtcgacgagg aaccgtccgg agcgaggccg ccgcgagccg
     4561 gcgaagccgg cgcgagacgg ccacgggcct cgtgcccgcg atggacgtgg tccccacgcg
     4621 cggcaccgtc gagacgacgc tgccggcgac gacgaggacc gtcgtcctcg ggctgtcccc
     4681 caccaccgac gacgtcgacc cggaggcatt cgccatgacg ctagagagag caacccgcga
     4741 cgagtccgac cccgtcgtcc tgcgcgtcgg cgaccggctc tacgggctct tcggcgtgac
     4801 gcccgacgag gacgccggga cgttggtcgc ggccgttcgg accgacgcgc gctgggagct
     4861 cgccggggtc ctcggcaccg aggacgatcc gcgcacggct gccgattgga tccggagcgg
     4921 ggggctgacg cgtctcgcgg gcctggtgac gacgcccgcg gggagcgcgc gcgtccgctg
     4981 gcgcgagccc gaatacgaga tggcccgatg agcggcccgc cgcctcccgg ccacatccgg
     5041 ctctacaccg cgatccgacc ggcgttgccg gccggcacgt acaggttgga gacgtcgcag
     5101 cggatcacgt acgactcaca aacgcagtcg ttgccaagcg ccggccagac catagagctc
     5161 gtcgccccac gcttccggat gtccggctcg gagctgttga gcacgttccc ccctgccttc
     5221 gcgcagggcc cgttctcgaa ccggttcgcg caggtcgcgc tgaaacgccg cacgctcccg
     5281 tgggaacgcg accccggtgc gggggccgac gggcgcaaga ccccgtggct gatgctggtc
     5341 gtcctggcaa agggcgaggg agagctcgag ccgaacaccc cgctgtcgtc tagcgatctg
     5401 gcggaactac gcacggcggt cggcctctct caggccgagg cggccgaggc cgggtcgtgc
     5461 gacgtgctgc gcgtcacggg ccgcatcgtc cgcaagtgct tcccgagccg cacggacctg
     5521 ccgtacctcg cgcacgtccg cgaggtcaac ctcctcgaca ccgagacggc tgccggggac
     5581 gacgacgggt tcgtggcaat cgtgacgtcg aaccgggtgc tgcgcggccg tcagagctac
     5641 acggcttacc tagtgtccgt cgaggggcag ctgggcgcgt tgcagggccc gcgcgaaacc
     5701 accgacgagg tcggcccggc cgcgaccacg ccgggagacc cggggggcgg agggggccgg
     5761 gacctcttga tgattaagga acatcccgtc gcgaacctgt tcgcgagcga tgcagtcaag
     5821 tcagccgcaa gggacgcggg caggagcgac gtcaagcgcg tgacggagca ccagcgcgag
     5881 cggctccagg ggtgggacga cccggtcgac gccgtcgggg gggccggcga gcacctcgtc
     5941 tcacacgagc tgttcgcgca cgcgaaggac cgcgggatga tcgtagagct cggccacatc
     6001 gaggtcctgg cagcggccga caccgaaccc ggcgacgacc gcgtccaccg gttcccggtg
     6061 ctcgcccatt ggaccttcca gaccatcggc aacggcgact tcgagacggt gatgcgcgga
     6121 ctcgacgtgg gcctgctcgg gaccgtcgac gccgacgcgg aggacgcccc tacggtcgtg
     6181 ccgaccggcc acgtgctgat cgaccgccgg acccggaagg gcgaagtctc ggccgggtgg
     6241 taccgcggcc ccctcgtccc gcacgagctc gaggaccgcg acgacgtgcc tctgcacgcg
     6301 gccgagcagg ccctgcggat cggcgccgac gggatcgaag acctgtcgta cgcgagcgcg
     6361 ttcgagctcg gccggctgat ggcgctcgcg gacccgaggt tcgtggccgc gctcgtcgcg
     6421 tggcgtcgca gaggcgcgac ccggcggtgg cgacggctca gctccgagct gttcgagacg
     6481 gggttgctgc cgtcgctgcc gctcgacaag ctcgaggggc tcggcatcga cgtcgacctc
     6541 gtggaccggc tgatcgcgtt cgaggagccc aacgacccgt tcggcccgtt gggcccgctc
     6601 gtcgacccgg tgccgtggcg gggcctctac gacccgcgag aggacgtcgc cgtgttgcgg
     6661 accatcggca tcgagcgtgc ccagatcgag gacgcgctgg gcatcggcgt ggcgtcgggc
     6721 ttcggctccg gtgaggagct ggtccgcgcc gacgtcgacc cgatggactt cgacacgctg
     6781 gccgagcgcg gaggcggcga gatggcggac gagctggaac ggcaccatca ggtcgacctt
     6841 cggggcatcg agagcctggg gggcgagtcg tgacgaccca ctggttcaag tcgcaaggag
     6901 tcgtccccgg gctgcgcaag gcgtgggccc cggaggacgc ggacgacgac gtggccgcag
     6961 cgccggccga ggtcctcgac tggctcgcgc ggctgcggct gctgcacggc gtgcccttcg
     7021 cctacctggt cgtggacgac gacctgctcc cacccgagag catccgcttc ttctggatgg
     7081 accgcgactg gaccgacgcg gccgtcgaag gcgccctgag cgccgccggc gcgcacagcc
     7141 tcgcccgcac cctcgccgcg accgaggatg ccgctctgag gcagcgcctc gaccagcgcg
     7201 aacgcaacat cacgccgcac gatcctcgcc tcgcgcaggc cgagcgtccc cccggcggag
     7261 ggccgacacg caccgacgac caccacgccg gccacctgcc ctacgacacc ctgtccggtc
     7321 cggccggcac cgtcacggga ttcgtcctcc gatccaaggc ggtttcgggg tggcccgagc
     7381 tgcacgtgcg cgcgctggac gaccgcggga acacgatgac gctgttgcgt gtcgaacgcc
     7441 ttgcacccgc cgtgctgctc gtgctgatcg acggctgccc gaacgaggtc cagatcgagg
     7501 agccgcggcg cggcatccag ttcggagtgg acctcacgcc gaacacggtc ccggccggcg
     7561 aggagcatcc cgacgagcac cccgacggtg ggcgccaggt cgcgctgcgg gacccggaga
     7621 acgggctgac cgtgctgctg aaagcaggtg cccgggtccc caatccgccg gccggaagcc
     7681 ccgacccgtc aggcagcaag cggacccagg tggcggtgcc gttccgcgag aagaccaccg
     7741 gtgtcgtcga catggcagcg ctggccgcgc acttcgccgg tgcggtcgac gccaacccgg
     7801 gcgccggcct gcagttcacg agcgacgacg acgaggaggc ctggccccac atggtcagca
     7861 cggccgagct gaccacccag ctgctgcagt tcccgtacca gcaacgattc cacggcgacg
     7921 gccacgatcc cgacggtggc cgtgtgggag ccgtcctggc cgtcacgacg ccggtcgaga
     7981 tcgtcgagat gttcacggcc ttcggccacc tggagtgagt gaggagacgt aatgcgtatc
     8041 gatccacggg accccgcgtt cgacccgaag gtcgtgtggc gacgcaactt ggacatcgcc
     8101 gtcgaccccg ctgcgctggt gttcgaccgg gacgtcagac ccttcgacgg ggatgggccc
     8161 gcgctgctgg tcccggtaga cgtccaggcc ctgagcgtcc cggaagacgg ggccgtcgac
     8221 cacgccgaca cgtcggtccg cctcgtcgac gcgcaggagc tgggcgatct cgccgaggcc
     8281 acggccgcgc cgttcaccga gggcgttggg cgctcggcgg ggatccacct ccactgggcg
     8341 atgcccgatg cgctgacgcg cgggacgacc tccgaaagcg gagtgctgtc ccagccgtcc
     8401 ctgcccgatc gatggctggt ggtgcgactc ggccccggaa cggaacgccc gtgggccggc
     8461 tgggtgatcg aatccgacta cggccgggcg accccgctgt cgcagtggga gggcgagccg
     8521 ggcgaccccg acggcgaccc gcccgcgggg tggatcccgc cggagagcct gtccgccgcg
     8581 tccggcggcg acccggcatg ggccgcgacg ttcgacaacg tcgtgaaccg gttcgcgttc
     8641 cacgatccgc tgaccgacgt cgacggcgac ctgttcacct acgccgtcgt cgggtggtac
     8701 ggggacgact cgctcgaccc gttggacgac gtgcggagca tgggcagctt cgaccacgtg
     8761 atgcagtcgc tgggctggac cgtctcccca gaggcgcacg agcgagccag gctcgagggc
     8821 ctgatccgca gcaatgcctt cgcgaccgcg ggggccgagg ggcgcgcgtt tcgcaaggtt
     8881 gcaaaccgcc gcgagcttgc ggtcggcgcg gccttcgacg acctcaccgt cgacgcgctg
     8941 gactcggtgt ggccgcgccg gtgcgtgttc cacggcgccg tgttcggcgt ccgtcgcgac
     9001 ggggtcgggg ccgaccatcg acccaccccc cacgacctga gcgcggcgct cggggccacc
     9061 gcgaccgaag cgctggcgcg gctgatcggc gaccgtcatc cgcagggggg agccgtggag
     9121 gaacgcgctt tggccgcgtt cgcgtacggg atagccgatc ggttggggga gcccgacggg
     9181 ccggcgctgg tcgaggaaca gatgcacggc cgggcgttcg agagcaggcc cgggccgcct
     9241 ctgcacgact ggatccgccg cacggaccgg ctgaaggacg tccggccgcc gaagatccgg
     9301 ccggcgcggg aggacgtctt gggcgggatg ctcgacgagg ctgcgatcac caggaaagac
     9361 gtcgcctgga aggcgaggtt cgacttcctc cccgatgccg acaaggaccg catccacgag
     9421 gtcggaggcg tggacgtagg caagaggttg caggctgcgg tccgcgagat cgaccgcatc
     9481 gagaaggtga cgcgcgcggg cccgcggtac ttccagccgc aggacccggt cgtcgcgatc
     9541 caggggtccg aacgccacct gcggcacggc tacgacggcc gctggaccgc cgacgagacc
     9601 gtcgcctgcc gtcttgccgg tgacacgcag gacgagtggg acgacctgac cggtgcggag
     9661 ctgctcgcgt ccccgttcgt gcatgccgcg ctgccgcccg aggctcgcca tctcgtccac
     9721 gaggccgccc tcgaagaccc ctccaacgtc gaggagacga tcgccgccgc caccaggcgc
     9781 gacccgaacc gggcagggga gatcgccgcg cgcatacgcg cagaggtggt catcggccgc
     9841 tacgggcgcg acccggaacg tcaaaccgac gcgctggcga agggatcctc acggcacggg
     9901 acgatgtcgt ccgggatcgg cttcaacgag tgggacgggc cgccgtggat cccgctgcat
     9961 ctcgaatgga tgatcgagct cgacgtcggc gatcgcctcg gcggctggga gctgggcgag
    10021 ctcgacttcg acccgccgaa gtccgtcgac gccgacccga cgaaggtcct
//
LOCUS       DASQDO010000552         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_853087, whole genome shotgun sequence.
ACCESSION   DASQDO010000552 DASQDO010000000
VERSION     DASQDO010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853087"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VEV43_15175"
     CDS             complement(<1..537)
                     /locus_tag="VEV43_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24908.1"
                     /translation="MKRRLVAVLLLSTLAGLGVAPAVAQDPEPSVAVDRIAFGELPSI
                     AVPLDGFMWVLDRRGAAIGKIDPDTNELVDVIDLPELVEGTRSAWDLEAARGSLWLTI
                     PSKKRIAEIDPDTHEVRSLLRTRGFVSNVDSARGSLWFVRGTERRTVLVRVDPASRKT
                     VATFRLGGHNTGVGGLTAF"
     gene            620..1222
                     /locus_tag="VEV43_15180"
     CDS             620..1222
                     /locus_tag="VEV43_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016933925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24909.1"
                     /translation="MRRFAVFFVTSVLAGCSAGGAERARPPAVAAATPVPANPEPSGE
                     PRDWRRPRYVPVREAVDRLARHVDVPVVLPRHGVFLRSYRGWLADPKHLSWSRRGDET
                     VGSLHLISRRDYLWISYGYAVPDGCGDRSSAVPTEVLGEPALLWHHSGDHSTIFWPVR
                     PHGRIGRFGLSGSFLGHEIHRLAESMETQIRAAPRHDPGC"
     gene            1394..2338
                     /locus_tag="VEV43_15185"
     CDS             1394..2338
                     /locus_tag="VEV43_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24910.1"
                     /translation="MRLEAYQHGIYPRSERLVAATRDLDRGRTDQAGVDAEVGRDRER
                     FVEAQRDAGLDYLSDGMLRWQDLFRPFVDAAGGLAPGPLTRWFDTNSFFRAPVVTGGL
                     SLDGGAVSFEDVGGVPRVVSLPSPDAFSRAAHFDGDRDALMLDVARDVLAPVAAEAVR
                     SGAAIVHLEDPWLTFYGIEDGAWGPLAKSIDELRSGFDATVVFHTYFGDAAPHIERLQ
                     GLPVDAVGIDLVASDTASIQLRSGIGVLLGCFDGRSSVVETADALVPALRKLLDRLDP
                     STCFLSSSGDLQLVSEEIARRKVGVLGEVASALKEEVA"
     gene            2335..>3085
                     /locus_tag="VEV43_15190"
     CDS             2335..>3085
                     /locus_tag="VEV43_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24911.1"
                     /translation="MNRPLLMHEVGSLGKPPWRVKPFSAGRVDAADVEDARTWGERLG
                     VPGYEELIELLERGEWGGDEKLRLKQWSSVYALRFFESVGLDVIYDGEQQRSEMYAHT
                     VAHATGFEQRGSVRSFDNKYYTKSAVVDRPGMREAYHDDEFSFLKSSTDSELKVPVTG
                     AYTVGAWSFDEHYARGLDLSLPLEERRRKTADARREMILDIARNVIRPCLESLIGLGA
                     RWIQLDEPAASTVPDELDLVVDSFNASVEGLE"
BASE COUNT          433 a         1118 c         1078 g          456 t
ORIGIN      
        1 gaacgcggtg agcccgccga cgcccgtgtt gtgtccgccg agccggaacg tcgcgaccgt
       61 cttgcgcgat gccgggtcca cgcgcacgag gaccgtgcgc cgctcggtgc ctcgcacgaa
      121 ccacaacgac ccgcgggccg agtcgacgtt gctgacgaac ccgcgcgtgc ggaggagcga
      181 gcggacctcg tgcgtgtcgg ggtcgatctc cgcgatgcgc ttcttgctcg ggatcgtcag
      241 ccacagagag ccgcgtgcgg cctcgaggtc ccaggcggag cgggtcccct cgacgagctc
      301 ggggaggtcg atcacgtcga cgagctcgtt cgtgtcaggg tcgatcttcc cgatcgcggc
      361 gccccggcgg tcgaggaccc acatgaagcc atcgagcggg accgcgatcg aaggcagctc
      421 gccgaaggcg atccgatcga cggcgacgga cggctcggga tcctgcgcta ccgcgggcgc
      481 gacccccagg cccgccagcg tcgacagcaa caagacggca accaatcgtc gcttcatcgt
      541 gtccccccca ctagtcgtcg gccgcgtcga gtctcccacc gcgggcgaac gttcgctctg
      601 cccgcgcagt ctcaagaacg tgcgacggtt cgccgtcttc ttcgtgacgt ccgtgctcgc
      661 cgggtgctcc gccggcggcg cggaacgcgc gcggcccccg gccgtcgcgg ccgcgacacc
      721 cgtaccggcc aaccccgagc cctccggaga gccgcgcgat tggcgcaggc cgcgctacgt
      781 ccccgtccgc gaggccgtcg accggctcgc ccgtcacgtc gacgttccgg tcgtcctgcc
      841 gcgccacggc gtcttcctcc ggagctaccg gggctggctc gccgacccga agcacctcag
      901 ctggtcgcgc cgcggggacg agaccgtggg cagcctccac ctgatctcgc ggcgcgacta
      961 cctctggatc tcgtacgggt acgcggtccc ggacgggtgc ggcgaccgca gcagcgcggt
     1021 tcccaccgag gtcctgggcg agcccgctct cctgtggcac cactcgggcg accacagcac
     1081 gatcttctgg ccagtccgcc cgcacggccg gatcgggcgg ttcggtctgt ccgggtcgtt
     1141 cctcggacac gagatccacc ggctcgccga gtcgatggag acgcagatcc gcgcggctcc
     1201 ccggcacgat cccggctgct gatacaccgg cacgcgcgaa gggacgtcgc tgcggcgcgt
     1261 ccgggcccgc cccgcagggt ccctcgataa cggcaggcgc ccggcgaccc cgccatctac
     1321 ccgccacgtc ggcaagaaag caccggcccg gcggccgcgc tggtctaggc tctcgcaacc
     1381 tagggagggc gcgttgcggc tagaggcgta ccagcacggg atatatccgc gttcggagcg
     1441 attggtggcg gcgacccggg acctcgaccg cggccggacc gaccaggcgg gcgtcgacgc
     1501 ggaggtcggg cgagaccgcg aacggttcgt cgaggcccag cgcgacgccg ggctcgacta
     1561 cctgtcggac ggcatgctgc ggtggcagga cctgttccgt ccgttcgtgg acgcggccgg
     1621 cgggctcgcg cccggccccc tcacgcgctg gttcgacacg aactcgttct tccgcgctcc
     1681 ggtcgtcacc ggggggctgt cgctcgacgg cggcgcggtc tcgttcgagg acgtcggcgg
     1741 cgtcccgcgc gtcgtcagcc tcccgtctcc ggacgcgttc tcgcgggcgg cccacttcga
     1801 cggcgaccgt gacgccctga tgctcgacgt cgcccgcgac gtcctcgcgc cggtggctgc
     1861 cgaggcggtc cgttccggcg cggcgatcgt ccacctcgaa gacccgtggc tcacgttcta
     1921 cgggatcgag gacggcgcgt gggggcccct cgcgaagagc atcgacgagc tgcgcagcgg
     1981 cttcgacgcg accgtcgtgt tccacacgta cttcggggac gccgcccccc acatcgaacg
     2041 gctgcaaggt cttccggtag acgccgtcgg gatcgacctg gtggcctccg acaccgcttc
     2101 gatccagctt cgttccggga tcggcgtgct gctcggctgc ttcgacggcc ggagctcggt
     2161 cgtcgagacc gccgacgccc tcgtccccgc gctgcggaag ctcctcgacc ggctcgaccc
     2221 gagcacgtgc tttttgtcgt cgagcggcga cctgcaactg gtgtcggagg agatcgcccg
     2281 tcgcaaggtc ggggtcctcg gcgaggtcgc ctcggcgttg aaggaggagg tggcgtgaac
     2341 agaccgctgc taatgcacga ggtcggctcg ctcggcaagc cgccgtggag ggtcaagcct
     2401 ttcagcgcgg gccgcgtgga cgcagccgac gtcgaagacg cgcgcacctg gggcgaacgg
     2461 ctcggcgtcc ccggctacga ggagctgatc gagctgctcg aacggggcga gtgggggggc
     2521 gacgagaagc tccggctcaa gcagtggtcg agcgtctacg cgctccgctt cttcgagtcc
     2581 gtcggcctcg acgtgatcta cgacggcgag cagcagcgca gcgagatgta cgcgcacacg
     2641 gtcgcgcacg ctacgggctt cgaacagcgt gggagcgttc ggagcttcga caacaagtac
     2701 tacacgaagt ccgcggtggt ggaccggccc ggcatgcgcg aggcgtacca cgacgacgag
     2761 ttctcgttcc tgaagtcgtc gacggactcc gagctgaagg tgccggtcac cggcgcgtac
     2821 acagtcggcg cgtggtcgtt cgacgagcac tacgcccgcg ggctcgacct ctcgctcccg
     2881 ctggaggagc gccggcgcaa gaccgccgac gcgcggcgcg agatgatcct cgatatcgcc
     2941 cgcaacgtca tccggccctg tctcgagtcg ctgatcggtc tcggcgcgcg ctggatccag
     3001 ctggacgagc ccgccgcctc cacggtgccc gacgagctcg acctcgtcgt cgactcgttc
     3061 aacgcgtccg tcgaaggcct cgaag
//
LOCUS       DASQDO010000553         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_180246, whole genome shotgun sequence.
ACCESSION   DASQDO010000553 DASQDO010000000
VERSION     DASQDO010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180246"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VEV43_15195"
     CDS             complement(<1..864)
                     /locus_tag="VEV43_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24912.1"
                     /translation="MERPKRATGLLLVAVLGIGGAAVAAAAGGAQGPRDVTLQRDSSD
                     DDEDVGTQATTPPTAPPTSDPGRVRGDDRGDRRDDGPTDDPGEADFATTPPTATPDDD
                     DFATAPPTATPDDDDDDRDDNSGPGNGDDDRDDNSGPSENSGPGNAGDDDRDDDRDDD
                     DRDDDGRDDDGRGNGKGRGHGQRPDPTPEPAPSEPAPSEPAPSQPSPDKPTPSDPAPS
                     NPAPDKPAPAGPSPSKPAPGRPTPSDPAEPGSSRPTPSSGPTGPGRSDSQPSAGPGTS
                     SGPGSSGPSSGA"
     gene            967..1524
                     /locus_tag="VEV43_15200"
     CDS             967..1524
                     /locus_tag="VEV43_15200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24913.1"
                     /translation="MEEAQLRALVKKASRGDEDAAALLFDEYYPRVYKYAAARLGPGP
                     SAEDAASETFAKVVRELGRFKWRGAGFEGWLFRIARNVIVDMQRRTGRETLTEEMFEP
                     SETSQEGLPETALVNVESATELRALLDRLPPEQREVLLLRFGAGLDTNETGRAMDRNA
                     NAVRQLQLRALRNVRAMLPEGVKGA"
     gene            1521..2318
                     /locus_tag="VEV43_15205"
     CDS             1521..2318
                     /locus_tag="VEV43_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24914.1"
                     /translation="MNEHEKDVQAEELADLARRLERALETDTPEARSEKALFVAGVGA
                     RARRSSWSAALIPATAVVAVLIALAVVSRGALPGQALYPFRKALDRVGLAPSTAGEVR
                     ELLDEAGRLVTAAEGAATSSPSRARALAIDALATLREADRLVGELDGERADELEREIE
                     ALEDRADEAEDLADDTLDEREDAREEAEEREDSSGSGSGGDDDDSSGPGSGGEDDDSS
                     GSGSGGDDDNSSGSGSGDDDDSSGSGSGGDDSSGSGSGGDDDSSGKG"
     gene            complement(2315..2848)
                     /locus_tag="VEV43_15210"
     CDS             complement(2315..2848)
                     /locus_tag="VEV43_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYP24915.1"
                     /translation="MIPIFDLDGTLLDSDAALTAPFVRLGIPAESVSFGMRLDEACAR
                     VGITVDQYLAAYDSGFAEPFAGVSEMLAALPRWAICSNKLGDYARQELDALGWEPEVA
                     LFAERFEGPKRLGPVLDELGLRGEEVLYVGDSEHDRTCAREAGVRFALAGWNPRVTAE
                     DGDLVLSAPADVLSALR"
     gene            complement(2861..>3543)
                     /locus_tag="VEV43_15215"
     CDS             complement(2861..>3543)
                     /locus_tag="VEV43_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HYP24916.1"
                     /translation="GIKAWDHDLDASTPQEELLDLLARLNEDAAVHGILVQLPLPEHI
                     DEETVLNAVLPSKDADGFHPCNLGRLVTGDHVVAPATPAGIQEMLVRTGVETTGAEVV
                     IVGRSNIVGKPLAALMVQKAAGANATVTLCHTGTKDLAAHTKRADILVAAIGRAQAIT
                     GPMIAPGATVIDVGINRTEDGLVGDVDFAAASEIAGAITPVPGGVGPMTIAMLLSNTV
                     KLAERAGA"
BASE COUNT          456 a         1226 c         1337 g          524 t
ORIGIN      
        1 tgccccgctg gagggccccg acgaaccagg accgctcgac gtgcccgggc cggcggaggg
       61 ctgcgagtcg gaccggcccg gcccggtggg acccgatgac ggtgtcggcc ggctcgaccc
      121 aggctcggcc gggtcggaag gggtgggcct gccgggtgcc ggcttcgacg gcgacgggcc
      181 tgctggtgcc ggcttgtcgg gggccgggtt cgacggggcc gggtccgaag gggtcggctt
      241 gtcgggtgac ggctgcgagg gtgccggctc ggagggcgcc ggctcggacg gtgcgggctc
      301 cggggtcggg tccggccgct ggccgtgtcc cctgcccttg ccgttgcccc ggccgtcgtc
      361 gtcccggccg tcgtcgtccc ggtcgtcgtc gtcccggtcg tcgtcccggt cgtcgtcgcc
      421 ggcgttgccc ggcccgctgt tctcgctcgg cccgctgttg tcgtcccggt cgtcgtcacc
      481 gttgcccggt ccgctgttgt cgtctcggtc gtcgtcgtca tcgtcgggcg tcgcggtcgg
      541 gggggccgtc gcaaagtcgt cgtcgtcggg cgtcgcggtc gggggggtcg tcgcaaagtc
      601 ggcctcgccc gggtcgtcgg tagggccgtc gtcccggcgg tcgccccggt cgtctccgcg
      661 aacgcggccc gggtcggacg tcggcggcgc ggtcggcggg gtcgtcgcct gagtcccaac
      721 gtcctcgtcg tcgtccgacg aatccctctg cagcgtcacg tccctcgggc cttgcgcacc
      781 cccggctgcg gccgccaccg cggccccgcc gatgccgagc acggccacca gcagcaggcc
      841 cgtcgccctc ttcggtcgct ccatcggaat actccttccg tcgtcctgtt cgaccgtcta
      901 acgggcgacg cccacgctgc gttatgccgt aacgcggcga cgaggacgtg cgtttagaag
      961 gtggatgtgg aagaagctca gctcagggcc ctggtcaaga aggcgtcgcg cggcgacgag
     1021 gatgccgccg cgctcctctt cgacgagtac tacccgcgcg tctacaagta cgccgcggcg
     1081 aggctcggcc cgggcccttc cgcagaggac gccgcatccg agacgttcgc caaggtggtc
     1141 cgtgagctcg ggaggttcaa gtggaggggc gccggcttcg aagggtggct cttccgcatc
     1201 gcccggaacg tgatcgtcga catgcagcgc cgcaccggac gcgagacgtt gacggaggag
     1261 atgttcgaac cgtccgagac gtcacaggaa gggttgccgg agactgcgct ggtgaacgtc
     1321 gagtccgcca ccgagctgcg cgctctcctc gaccgtcttc cgccggagca gcgcgaggtc
     1381 ctgctgcttc gtttcggagc cgggctcgac acgaacgaga cgggccgcgc catggaccgg
     1441 aacgcgaacg cggtgcggca gctgcagctc cgggccctcc gtaacgtacg ggcgatgctc
     1501 ccggaggggg tgaagggcgc gtgaacgagc acgagaagga cgtgcaggcc gaagagctcg
     1561 ccgacctcgc gcgccggctc gagcgggctc tcgagaccga cacgccggaa gcgcgcagcg
     1621 agaaggctct gttcgtcgcc ggagtaggcg cgcgggcgcg gcggtcgtct tggtccgcgg
     1681 ccctgatccc cgcgacggcg gtggtcgccg tcctgatcgc gctggccgtg gtgtcgcgcg
     1741 gggccctgcc gggtcaggcc ctctatccgt tccggaaggc gctcgaccgc gtcgggctcg
     1801 cgccgtcgac cgcgggcgag gtgcgcgagc tgctcgacga ggcggggcgc ctcgtgaccg
     1861 ccgccgaggg ggccgctact tcgtcgccgt cccgggctcg ggcacttgcc atcgacgcgc
     1921 tggcgacgct ccgcgaagcg gaccggctcg tcggcgagct ggacggcgag cgcgccgacg
     1981 agctcgaacg agagatagag gcgctcgaag accgggccga cgaggccgaa gaccttgccg
     2041 acgacacgct cgacgagcgc gaggacgccc gggaagaggc cgaggagcgt gaggactcga
     2101 gcggcagtgg ctcgggtgga gacgacgacg attcgagcgg gcccggctcc ggcggggagg
     2161 acgacgattc gagcggcagc ggctccggtg gagacgacga caactcgagc ggatccggct
     2221 ccggcgacga cgacgattcg agcgggtcgg gctcgggtgg ggacgactcg agcggcagcg
     2281 gctccggcgg ggacgacgac tcgagcggca agggttaacg caaggcgctc agcacgtcgg
     2341 ccggcgcgct caacacgagg tcgccgtctt ccgcggtgac cctcgggttc caccccgcga
     2401 gcgcgaagcg gacgcccgcc tcgcgcgcgc aggtgcggtc gtgctcggag tcgccgacgt
     2461 agagcacttc ctcgccgcgc agccccagct cgtccagaac cggcccgagg cgcttcggcc
     2521 cctcgaacct ctcggcgaag agggctacct cgggctccca gcccagcgcg tccagctcct
     2581 gccgagcgta gtcgccgagc ttgttcgagc agatcgccca gcgcgggagg gcggcgagca
     2641 tctcggacac gccggcgaag ggctcggcga acccggagtc gtaggcggcg aggtactggt
     2701 cgaccgtgat gcccacgcgg gcgcaggctt cgtcgagacg catcccgaac gacacgctct
     2761 cggcagggat ccccagccgg acgaacggcg cggtgagcgc ggcgtcggag tccaggagcg
     2821 tcccgtcgag gtcgaagatc gggatcaagc ggtgcagggc ctaggccccc gctcgttccg
     2881 ccagcttcac cgtgttgctc agcagcatcg cgatcgtcat cggcccgacg cccccgggga
     2941 ccggtgtgat cgcaccggcg atctcggacg ccgccgcgaa gtcgacgtcg ccgacgagac
     3001 cgtcctcggt cctgttgatg ccgacgtcga tgacggtcgc gcccggagcg atcatcgggc
     3061 cggttatcgc ctgcgcgcgt ccgatcgccg cgacgaggat gtcggcgcgc ttcgtgtgtg
     3121 cggcgaggtc cttcgtcccc gtatggcaca gcgtcaccgt cgcgttcgcg cctgcggcct
     3181 tctggaccat cagcgcggcc agcggcttgc cgacgatgtt cgaacggccg acgatcacga
     3241 cctcggctcc cgtcgtctcg accccggtgc gcacgagcat ctcctggatg cccgccggcg
     3301 tcgcgggcgc gacgacgtgg tcgcccgtca cgagccggcc gaggttgcag gggtggaagc
     3361 cgtcggcgtc cttggacggg agcaccgcgt tcagcacggt ttcctcgtcg atgtgctcgg
     3421 ggagcgggag ctgcacgagg atcccgtgga cggccgcgtc ctcgttcagc cgcgcgagca
     3481 ggtcgagtag ctcttcctgc ggcgtgctcg cgtcgaggtc gtggtcccag gccttgatgc
     3541 cga
//
LOCUS       DASQDO010000554         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_914468, whole genome shotgun sequence.
ACCESSION   DASQDO010000554 DASQDO010000000
VERSION     DASQDO010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914468"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..442)
                     /locus_tag="VEV43_15220"
     CDS             complement(161..442)
                     /locus_tag="VEV43_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24917.1"
                     /translation="MTEQGRAARYSDLFGDRTEHPSDCQYCPFCATVNVVRKTHPEVL
                     EHLAAAARELIAAAGIMIEEAAKTVAPPPAAEPEQAPDDVPAVRRIEAV"
     gene            complement(480..917)
                     /locus_tag="VEV43_15225"
     CDS             complement(480..917)
                     /locus_tag="VEV43_15225"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYP24918.1"
                     /translation="MAESVKDSIDVEASAEQIFEVATDFEAYPEWNANIKRVEVRETD
                     SEGRGTKVFYEVDAKVKVVRYTLSYDYSDAPRSFSWDLLDGDVKELSGSYAFDEFDDL
                     TEVVYETAVDPGFPIPKLLKRQAQRAIVRGALEDLKKRVESKA"
     gene            complement(920..1696)
                     /locus_tag="VEV43_15230"
     CDS             complement(920..1696)
                     /locus_tag="VEV43_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYP24919.1"
                     /translation="MPVYVVADLHGAAEELKAAVPEHGPLLLLGDLVNFLDYISMTGI
                     LTEVFSIETVSEVVELRTAGKVEEARVVMRERSRGREDEIRAEIGRRVTEQYQEVFAA
                     LPDPTHLILGNVDNPMLAESFASATPGAHLLAAGVVTIGGERFGLVGGALPTPLNVAG
                     EISHDEMRARVSSLGEVDVLCSHIPPAVPELCFDTVSGKREQGSDDLLEYIRDVQPRR
                     AYFGHVHQPLVSVTTIGRTVCVNVGYFRATRRAFVHHEGS"
     gene            1720..>2423
                     /locus_tag="VEV43_15235"
     CDS             1720..>2423
                     /locus_tag="VEV43_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HYP24920.1"
                     /translation="MSVVFVHGGVAGVAKTERVSLARGVELGVAHERALDAAEAAVVA
                     LEDDPRLNAGYGAVLNLAGEVELDAGISDGFAGRSGGVGNVRVAHPISLARKVLEETP
                     HVLLTGEGAASFGREMEPVEIAPEELRRWEEARAEGRLDVARFGDPVEVDTVGAVALG
                     AGGDLAAASSTGGVFGKMPGRMGDSAIFGAGHYASKAAAVVGTGVGEMFLETLACARA
                     GMLIEAGAHPQEACVEV"
BASE COUNT          348 a          823 c          878 g          374 t
ORIGIN      
        1 cgcgacgagg ctcttggttg cagccgacgg gtcggtggga cgctcgacgc gttcgaccac
       61 ggcaccatct cgcccgacgc cggccgcctt gacggcggtc ccgccgacgt cgacgccgac
      121 tacgacctgc gctctctcag ccatccagcc gaggatacgg ctagacggcc tcgatcctcc
      181 tcacggccgg gacgtcgtcg ggagcttgct caggctcggc cgcggggggc ggggcgacgg
      241 tcttcgcggc ctcctcgatc atgatcccgg ccgccgctat cagctcgcgg gccgccgcgg
      301 ccaggtgctc gagcacctcg gggtgggtct tgcgcacgac gttgaccgtc gcgcagaagg
      361 ggcagtactg gcagtcggac ggatgctcgg tgcggtcgcc gaagaggtcg ctgtagcgcg
      421 ccgcccgccc ttgctccgtc accggcgcaa cctatccgtt ctcgcgatgg ccgaggccat
      481 cacgccttgc tctcgacgcg cttcttgagg tcctccagcg cgccgcggac gatagcgcgc
      541 tgcgcctggc gcttcaggag cttcgggatc gggaagccgg ggtcgaccgc cgtctcgtag
      601 acgacctcgg tcaggtcgtc gaactcgtcg aacgcatacg agccgctgag ctccttgacg
      661 tcaccgtcga ggaggtccca cgagaacgag cgcggcgcgt ccgagtagtc gtacgagagg
      721 gtgtagcgga cgaccttcac cttcgcgtcg acctcgtaga agaccttcgt cccccggccc
      781 tccgagtcgg tctctcgcac ctcgacccgc ttgatgttcg cgttccactc gggatacgcc
      841 tcgaagtccg tcgcgacctc gaagatctgc tccgccgaag cctcgacgtc gatcgagtcc
      901 ttgaccgact ccgccacgct cagctccctt cgtgatggac gaacgcccgc cgggtggcac
      961 ggaagtagcc cacgttgacg cagaccgtac gcccgatcgt cgtgaccgac acgagcggct
     1021 ggtggacgtg cccaaagtat gcccggcgcg gttgcacgtc gcgtatgtac tcgaggaggt
     1081 cgtcgctccc ctgctcgcgc ttgcccgaca ccgtgtcgaa gcacagctcg gggacggccg
     1141 gcgggatgtg cgagcagagc acgtcgacct ccccgagcga cgagacgcga gccctcatct
     1201 cgtcgtggct gatctcgccc gccacgttga ggggcgtggg caaggcccca ccgacgaggc
     1261 cgaaccgctc ccctccgatc gtcacgacac ccgccgccag caggtgcgcg ccgggcgtgg
     1321 cggacgcgaa cgactcggcg agcatcgggt tgtcgacgtt gccgaggatg aggtgggtcg
     1381 ggtccgggag cgcggcgaag acctcctggt actgctcggt gacacggcgg ccgatctcgg
     1441 cgcggatctc gtcctcgcgc ccccgggacc gctcgcgcat cacgacgcgc gcctcctcga
     1501 ccttgcccgc ggtccgcagc tcgacgacct ccgacaccgt ctcgatcgag aagacctcgg
     1561 tcaggatccc ggtcatcgag atgtagtcga ggaagttcac gaggtcgccg agcaggagga
     1621 gggggccgtg ctcggggacg gccgccttca gctcctcggc ggcgccgtgc aggtcggcga
     1681 ccacgtagac gggcatccgg gcatcgtaag gtcgccgtca tgagcgtggt cttcgtccac
     1741 gggggcgtcg cgggcgtggc gaagacggag cgggtctcgc ttgcccgggg agtcgagctg
     1801 ggtgtcgccc acgagcgcgc gctcgacgcg gccgaggcag cggtggtcgc gctcgaggac
     1861 gatccccggc tgaacgcggg ctacggcgcg gtgctgaacc tggccggcga ggtcgagctc
     1921 gacgccggca tctccgacgg tttcgccgga cgttctggag gcgtcggcaa cgtgcgcgta
     1981 gcgcacccga tatcgctcgc tcgcaaggtg ctcgaggaga caccgcacgt gctgctgacg
     2041 ggcgagggcg cggcttcctt cggccgtgag atggaaccgg tcgagatcgc acccgaggag
     2101 ctgcggaggt gggaggaggc gagggccgag ggacggctcg acgtcgcgcg cttcggcgac
     2161 ccggtcgagg tcgacacggt gggggccgtg gcgctcgggg ccgggggcga cctggcggcc
     2221 gcgtcgtcga cgggcggcgt gttcgggaag atgcccggcc gcatgggcga ctcggcgatc
     2281 ttcggcgccg gccattacgc gtcgaaggcg gcagcggtcg tcgggactgg cgtcggcgag
     2341 atgttcctag agacactcgc gtgcgcgcgt gccgggatgc tgatcgaggc gggagcccat
     2401 ccgcaagagg catgcgtcga ggt
//
LOCUS       DASQDO010000555         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_119294, whole genome shotgun sequence.
ACCESSION   DASQDO010000555 DASQDO010000000
VERSION     DASQDO010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119294"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VEV43_15240"
     CDS             complement(<1..680)
                     /locus_tag="VEV43_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYP24921.1"
                     /translation="MTERPLEVVSDGLRLDAGIDVPDRPRGTAVLLHGIPSISPPEPG
                     DLGYRGLAERFAAEGWAAAWGDMRAVRKSPGYFSIEGWVKDALAIVDAARVAAEGPVV
                     LVGSSAGGAVSTEAVRRGAPVDALVLLAAPAEWLSFADDPAMGVFRVQAGAGMELSPE
                     TLEDPAQWAAEFENVTAEISVASVRVPVLVVHGTDDDVVPVVHAPRFEAAAPAAQLEI
                     VEGAGHQLR"
     gene            complement(677..1057)
                     /locus_tag="VEV43_15245"
     CDS             complement(677..1057)
                     /locus_tag="VEV43_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYP24922.1"
                     /translation="MRRIAFLLVALALAACSGGGSSDGATVDMLEGQRFEPETLTVET
                     GTTVTFVNDSAEAHTVTAVDGVPVYFSSGGFESEDEARDNLADALIGQEDTYEVTFDE
                     PGTYEYVCIPHEDQGMTGTIEVEG"
     gene            complement(1068..2231)
                     /locus_tag="VEV43_15250"
     CDS             complement(1068..2231)
                     /locus_tag="VEV43_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HYP24923.1"
                     /translation="MAVEASRLRRSIGWLGPLALAAILVTLLFWLDPIGSLREVPPVE
                     EVAIERTVLNEGEIVLDLRNDGPDPVTISQVLVDEAYWTFDVGDATLGRLESTTLSIP
                     YPWDEGLPLHIALITATGVTIEHEIEAAALTPESDSGTLGVYALLGLYMGVLPVAIGI
                     LWFPSLRRASRSAIGFFLAFTVGLLAFLLVDTIGEGLELAGETGAALDGAGLFAIGAL
                     TALMALFFLEAELRRRSTSRAGLVLAYLIAAGIGLHNLGEGLAVGAALAAGEVALGTF
                     LVAGFALHNTTEGLAIVAPLGREGTRPGIKHLVALGALAGVPAIAGAWLGGFAFSPAP
                     AAFAFGVAAGAIAQVVWAVSRSMKGDESLTSRWGAAGVVLGLLVMYSTGLLTA"
     gene            complement(2232..3242)
                     /locus_tag="VEV43_15255"
     CDS             complement(2232..3242)
                     /locus_tag="VEV43_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYP24924.1"
                     /translation="MGLLDRRRFLKRTAALGALGSVAAVGGRDLVAAQGSHGHGGMDH
                     RAHGGMGVVGEVDHARNGFDPHEILTDFDFGEVSRDAQGRTVREYELVTVDKEIEVAP
                     GVFFPAWTYNGRVPGPTIRCTEGDRIRISLVNASSHPHTIHFHGIHSAGMDGIPGSGE
                     AQPGETFVYDFVAEPFGTHLYHCHSLPLRQHIHRGLYGGFVVDPKKGRPDANEMVMVM
                     NAFDTNFDGGNEVYAANTVAFAYHDKPIPLERGRLQRLHVINVTEFDPLNSIHIHANF
                     FQLYRTGTSLTPHEFTDTISMGQAERHMLEFTYDQPGQFMFHAHVTEFTELGWMGFFE
                     VT"
     gene            complement(3230..3904)
                     /locus_tag="VEV43_15260"
     CDS             complement(3230..3904)
                     /locus_tag="VEV43_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYP24925.1"
                     /translation="MALTTSPAAQDYLKALHSIEWEAGEPGPVSTTAVADRLGISAAS
                     ATNMLKKLDSMGLVVQVPYRGTELTESGRKIALEVVRHHRLLETYLAEALGVPWDEVH
                     AEAEVLEHVLSERLEDRIAALLGHPEWDPHGHPIPGKDGSMPPASRRTLWDGGDGERL
                     AVERVRDDDPEVLRYLAGVGVTPGAEVEVVKRGPVAGPLFVRVADRKEDVEALSRELA
                     EAVWVS"
     gene            3970..>4797
                     /locus_tag="VEV43_15265"
     CDS             3970..>4797
                     /locus_tag="VEV43_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYP24926.1"
                     /translation="MTNSARGPTPQRAAAQALAITVVLAAAKVAVWAATSSLAVLSQA
                     LDSVLDVVALALLLVGVRIASKPADTSHHYGHAKAENLVAFTQMLLLGGLVGAVVVEA
                     TGRLASGESDPTAPWYALALLGVSAVIDLWRARALLRVAREHRSHALEAGALNVASDV
                     GTALVALVSLAIVRAVVGGADAVGALVVSAAVVVAGVQVTRRSVDVLMDRAPGGPVEA
                     IEAAAARAAGVTETRRVRVRGSGDRVFADVTVAAGRTASLERAHDIAEAVEAEIEKVA
                     "
BASE COUNT          726 a         1723 c         1679 g          669 t
ORIGIN      
        1 cggagctggt gcccggcccc ctcgacgatc tcgagctgcg cagcgggggc agcagcctcg
       61 aaccgcggag catgaacgac cggcaccacg tcgtcgtccg tcccgtgcac gacgaggacc
      121 ggcacccgca ccgacgcgac ggagatctcc gcggtcacgt tctcgaactc ggccgcccac
      181 tgagccgggt cctccagggt ctcgggcgag agctccatgc ccgctccggc ctggacccgg
      241 aagacgccca tcgcggggtc gtcggcgaac gagagccatt ccgccggagc ggcgagcaac
      301 accagcgcgt cgaccggagc gccgcgccgc accgcttcgg tcgagacggc tcccccggcc
      361 gaggacccga cgaggacgac ggggccctcc gccgcaaccc gagccgcgtc cacgatcgcg
      421 agcgcgtcct tgacccatcc ctcgatcgag aagtagcccg gggacttgcg gaccgcgcgc
      481 atgtcccccc acgcggcggc ccatccctcg gcggcgaacc gctcggcgag cccccggtag
      541 ccgaggtcgc cgggctcggg cggcgagatc gaagggatgc cgtgcaacag aacggcggtc
      601 ccgcggggac ggtccgggac gtcgatcccg gcgtccagcc gcagcccgtc gctcacgacc
      661 tcgagcggac gttccgtcat ccctcgacct cgatcgtccc ggtcatcccc tggtcctcgt
      721 gcgggatgca gacgtactcg taggtcccgg gctcgtcgaa cgtcacctcg taggtgtcct
      781 cctggccgat cagcgcgtcg gcgaggttgt cccgggcctc gtcctcggac tcgaagccgc
      841 cgctcgagaa gtacacgggc acgccgtcga ccgcggtcac cgtgtgagcc tcggccgagt
      901 cgttgacgaa cgtcaccgtc gtgcccgtct cgaccgtgag cgtctcgggc tcgaaccgct
      961 ggccctcgag catgtcgacc gtcgcgccgt cggaggaccc gccgcccgag caagcagcga
     1021 gcgcgagcgc gacgagaagg aaagcgatgc gtctcatggc ctcgaagcta agcggtcagc
     1081 aggcccgtcg agtacatgac gaggagcccg aggacgacac ccgcggcccc ccagcgcgac
     1141 gtcagcgact cgtccccctt catcgaacgg gacaccgccc acacgacctg cgcgatcgcg
     1201 cccgccgcca cgccgaacgc gaacgcggcc ggcgcgggcg agaacgcaaa gcccccgagc
     1261 cacgcccccg cgatcgcggg cacgcccgca agcgcgccca gagcgacgag gtgcttgatc
     1321 ccgggccgcg tcccttcgcg gccgagagga gcgacgatcg cgagcccctc ggtcgtgttg
     1381 tgcaacgcga agccggcgac gaggaacgtg ccgagcgcga cctcgcccgc cgcgagcgca
     1441 gcccccaccg ccagaccctc gccgaggttg tgcagcccga tccccgcggc gatgagatag
     1501 gcgaggacga gcccggcgcg cgacgtagac cgccggcgca gctcggcttc gaggaagaac
     1561 aacgccatca acgcggtcag cgcgccgatc gcgaacaggc ccgcgccgtc gagcgctgcc
     1621 cccgtctcgc ccgccagctc gagcccttcg ccgatcgtgt cgaccaagag gaacgcgagg
     1681 agcccgacgg tgaacgcgag gaagaaaccg atcgcagagc gcgacgcgcg gcgcagcgac
     1741 gggaaccaca ggatcccgat cgcgaccggc aacacgccca tgtagagccc cagcagcgcg
     1801 tagacgccga gcgtcccgga gtcggattcc ggcgtgagcg cggcggcttc gatctcgtgc
     1861 tcgatcgtca ccccggtcgc tgtgatcagc gcgatgtgga gcggcagccc ttcgtcccac
     1921 gggtacggga tcgagagcgt cgtcgactcg agccggccga gcgtcgcgtc gccgacgtcg
     1981 aacgtccagt acgcctcgtc gacgaggacc tgcgagatcg tcacgggatc cggcccgtcg
     2041 ttgcggaggt ccagcacgat ctcgccctcg ttcaggaccg tcctctcgat cgccacctcc
     2101 tcgaccggag gcacttcccg caggctcccg atggggtcga gccagaacag cagcgtgacg
     2161 aggatggccg cgagcgcaag ggggccgagc catccgatcg aacggcgcag cctcgaagct
     2221 tcgaccgcca tctacgtgac ctcgaagaag cccatccagc cgagctcggt gaactcggtg
     2281 acgtgggcgt ggaacatgaa ctgaccgggc tggtcgtagg tgaactcgag catgtggcgc
     2341 tcggcctggc ccatcgagat cgtgtcggtg aactcgtgcg gcgtcagcga cgtgccggtc
     2401 cggtacagct ggaagaagtt cgcatggatg tggatcgagt tcagcgggtc gaactcggtg
     2461 acgttgatca cgtgcagacg ctggagccgg ccgcgctcga gcgggatcgg cttgtcgtgg
     2521 tacgcgaacg cgacggtgtt ggcggcgtag acctcgttac cgccgtcgaa gttcgtgtcg
     2581 aacgcgttca tcaccatcac catctcgttc gcgtccggcc gtcccttctt cgggtcgacg
     2641 acgaaaccgc cgtagaggcc gcgatggatg tgctggcgca gcgggagcga gtggcagtgg
     2701 tacaggtggg tgccgaaggg ctccgcgacg aagtcgtaga cgaacgtctc gccgggctga
     2761 gcctcgcccg agccggggat gccgtccatg cccgcggagt ggatgccgtg gaagtggatc
     2821 gtgtgcggat gcgagctcgc gttcacgagg ctgatccgga tcctgtcgcc ttcggtgcag
     2881 cggatcgtcg ggccggggac gcggccgttg tacgtccacg cggggaagaa gacgccgggc
     2941 gcgacctcga tctccttgtc gacggtgacg agctcgtact cgcgcacggt ccggccctgc
     3001 gcgtcgcggc tcacctctcc gaagtcgaag tccgtcagga tctcgtgcgg gtcgaagccg
     3061 ttgcgcgcgt ggtcgacctc gccgacgacg cccatgcccc cgtgagcgcg gtggtccatc
     3121 ccgccgtggc cgtgggagcc ctgagccgct acgagatcgc gtccaccaac ggctgcgacg
     3181 cttccgagcg cgccgagcgc ggccgtcctc ttcaggaacc gcctgcggtc taggagaccc
     3241 atacggcctc cgcaagctcg cgcgacagag cctcgacgtc ttccttacgg tcggccacgc
     3301 ggacgaacag cggaccggcc accggccccc tcttgacgac ctcgacctcg gcccccggcg
     3361 tcaccccgac gcccgcgaga tagcggagga cctcgggatc gtcgtcccgc acgcgctcga
     3421 ccgcgagcct ctcgccgtcg ccgccgtccc acagggtccg ccgagacgcg ggcggcatgc
     3481 tcccgtcctt gcccgggatc gggtgaccgt ggggatccca ctcgggatgg ccgagcaggg
     3541 ccgcgatgcg gtcctcgagg cgttcggaca ggacgtgctc gagcacctcg gcctcggcgt
     3601 ggacctcgtc ccacggaacc ccgagcgcct cggcgagata cgtctcgagg aggcggtggt
     3661 gacggacgac ctcgagtgcg atcttgcggc ccgactcggt cagctcggtc ccccggtacg
     3721 ggacctggac gacgagcccc atcgagtcga gcttcttcag catgttcgtc gccgacgccg
     3781 ccgagattcc gaggcggtcc gcgaccgccg tcgtcgagac cgggccgggc tcgcccgcct
     3841 cccactcgat cgagtgaagg gccttgaggt agtcctgggc cgcgggagaa gtcgtgagtg
     3901 ccatcgaatg gaatttagcc gagactaagt ttttcgacaa ctcggccccc gcccccctac
     3961 cctggcggga tgacgaactc cgcccgaggc cccacgccgc agagggccgc ggcccaggcg
     4021 ctggcgatca cggtcgtcct cgccgcggcg aaggtggcgg tgtgggctgc cacgtcgagc
     4081 ctcgccgtgc tgtcgcaggc gctcgactcg gtcctcgacg ttgtcgccct cgccctgctg
     4141 ctcgtcggcg tgaggatcgc ctccaagccg gccgacacct cccaccatta cggtcacgcg
     4201 aaggcggaga acctcgtcgc gttcacgcag atgctcttgc tcgggggcct ggtcggcgcg
     4261 gtggtggtcg aggccaccgg gcgcctcgcg tccggcgagt ccgaccccac ggccccttgg
     4321 tacgcgctcg ctctactggg cgtgtccgcg gtgatcgacc tgtggcgggc gcgggcgttg
     4381 ctgcgcgtcg cgcgcgagca caggtcgcac gcgctcgaag ccggggctct caacgtcgca
     4441 agcgacgtcg ggaccgcgct cgtcgcgctc gtcagcctcg cgatcgtgcg cgcggtggtc
     4501 ggcggtgcgg acgccgtcgg agcgctggtc gtctccgccg cggtcgtcgt ggccggcgtc
     4561 caggtcacgc gccgttcggt cgacgtgctg atggaccgcg cgccgggggg gccggtcgag
     4621 gccatcgagg ccgcagccgc gcgggccgcc ggcgtcacgg agacgcggcg cgtgcgcgtg
     4681 cgcggcagcg gggaccgggt cttcgccgac gtgacggtcg cggccgggcg cacggcttcg
     4741 ttggagcgcg cgcacgacat agccgaggcg gtcgaggccg agatcgagaa ggtcgcg
//
LOCUS       DASQDO010000556         4465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_731008, whole genome shotgun sequence.
ACCESSION   DASQDO010000556 DASQDO010000000
VERSION     DASQDO010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731008"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="VEV43_15270"
     CDS             <1..1500
                     /locus_tag="VEV43_15270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYP24927.1"
                     /translation="RSILIGATAGTVELSLVSEGTVLASTAELEGPTEVGSIGRETFE
                     ADIGGDASAKARLLDGGAMALVSSTAEAPIRAVSNKVLLYMAALLLLALGVVTGLAYL
                     LARLITQPLEELSAGAAAIAEGRFDHRIQVRSKDEVGQLAEAFNDMTDTLGETVSELS
                     SSRDQLRRAIRRVGDTLRSTHDMKQMLASVLNTTVDAIRADGAVLWMFGSTREELYPA
                     RGVNVDLDSLGRVKVGEGVVGLVAERSVNVLLPQQAGPRPTAAETAHPVTIAIPLYSQ
                     DRIMGVIAVHRDDPDDHFTEEEFDYVVFLGEQAGVAVENVLLHEEAQRLSITDGLTGV
                     WNRRYFLMQFPQALAAAQRFQRPFSVLMLDLDSFKAINDTHGHQRGDAILVEFAKRVT
                     RVIREVDTFARYGGEEFICLLSETDLHGARTTAEKVLDAIRSEPFGVPGEQPLTITVS
                     IGVASYPVHGDTYKTLVGAADRALYRAKQQGRDRVCTATEPEPPLKLAT"
     gene            complement(2342..2803)
                     /locus_tag="VEV43_15275"
     CDS             complement(2342..2803)
                     /locus_tag="VEV43_15275"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYP24928.1"
                     /translation="MQLLLNNPTTTIVTVEARHRLGFVDLTRHLVSEAARLGEADGFA
                     IAFARHTTCALLINELEDGAQADLAARLDQLFPPSIYYAHDDLAIRTQNLQPGERANG
                     HAHVAQMLMGGTSHTIPISGGTPLLGRWQRLLLVELDEPKPREVVLQVYGG"
     gene            complement(2788..3882)
                     /locus_tag="VEV43_15280"
     CDS             complement(2788..3882)
                     /locus_tag="VEV43_15280"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HYP24929.1"
                     /translation="MGHGARGASLRGRRRLPRPCRRLYRSIPRERSVPWLAPAGRAAL
                     TSFLLLVTFGGLFASADAAFAQIAEDVLVPDVGLDLLPARIFVVLAVAAGAGGLILMA
                     QRPQEEPSPEGARWRLATTLEWAGPLLALNVLFAAFVVVQVAVLFGGHGHVLDTAGLT
                     YAQYARAGFFQLVWIAALVLGVIALAVKLAPPDQERLRNALLGLLCGLTLVVLASALR
                     RMSLYEDAYGLTRIRVSVFAVVLWLAAIFVAVMLAGLFRRGSWLPRAVVALSVAGLIA
                     FNVANPDALIARSAVERFERTGEVDEIYLSTLSTDALPALADLPLEERACAAYEIAAR
                     NDPAPWSSFNLSRDRAPRLARPEDMRCSYY"
     gene            complement(4286..4465)
                     /locus_tag="VEV43_15285"
                     /pseudo
     CDS             complement(4286..4465)
                     /locus_tag="VEV43_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT          742 a         1532 c         1541 g          650 t
ORIGIN      
        1 cggtcgatcc tcatcggtgc gaccgcgggg accgtcgagc tgtcactcgt gtccgagggg
       61 accgtcctgg cgtcgaccgc cgagctcgag ggacccaccg aggtcgggtc gatcggccgc
      121 gagacgttcg aggccgacat cgggggcgac gcctccgcga aggcgcgcct gctcgacggt
      181 ggcgcgatgg cgctcgtctc gtcgaccgcc gaagccccga tccgagccgt ctccaacaag
      241 gtcctcctct acatggcggc gttgctgttg ctggcgctcg gggtcgtgac cggcctcgcc
      301 tacctcctgg cgcggctcat cacgcagccg ctcgaggagc tgtcggccgg tgcggccgcc
      361 atcgccgagg ggcgcttcga ccaccgcatc caggtgcgct cgaaagacga ggtcggccag
      421 ctggccgagg cgttcaacga catgaccgac accttgggcg agaccgtcag cgagctctcg
      481 tcgtcgcgag accagcttcg ccgcgcgatc aggcgcgtcg gcgacacgct ccggtcgacg
      541 cacgacatga agcagatgct ggcgtcggtg ctcaacacga ccgtcgacgc gatccgtgcc
      601 gacggtgcgg tgctgtggat gttcgggtcg acgcgcgaag agctgtaccc cgcgcgcggc
      661 gtcaacgtcg acctcgactc gctcggccgc gtgaaggtgg gcgagggcgt cgtcgggctc
      721 gtcgcggagc gctccgtgaa cgtcctgctg ccgcagcagg cgggcccgcg tccgaccgcc
      781 gccgagacgg cccaccccgt cacgatcgcg atcccgctct acagccagga ccggatcatg
      841 ggcgtcatcg cggtgcaccg ggacgacccg gacgaccact tcaccgagga ggagttcgac
      901 tacgtcgtgt tcctcggcga gcaggccggg gtcgccgtcg agaacgtgct gctccacgag
      961 gaggcccagc ggctgtcgat caccgacggt ctcaccggcg tgtggaaccg caggtacttc
     1021 ctcatgcagt tcccgcaggc cctcgccgcg gcccagcgct tccagcgacc gttcagcgtg
     1081 ctcatgctcg acctcgactc gttcaaggcg atcaacgaca cgcacggcca ccagcgcggc
     1141 gacgcgatcc tcgtcgagtt cgctaagcgc gtgacgaggg tcatccgcga ggtcgacacg
     1201 ttcgcgcgct acggcggtga ggagttcatc tgcctgctgt cggagaccga cctgcacggc
     1261 gcgcgcacga ccgccgagaa ggtcctcgac gcgatccgct cggagccgtt cggcgtcccg
     1321 ggcgagcagc cgctcacgat caccgtgtcg atcggtgtgg cgtcctatcc cgtgcacggc
     1381 gacacgtaca agaccctcgt cggtgcggcg gacagggccc tctaccgggc gaagcagcag
     1441 ggccgcgacc gggtgtgcac ggcgaccgag ccggagcccc cgctcaagct cgctacctag
     1501 tcttcgagta gctacgtctg ggagccagga gacccaccgg gatccagtca catcgtgggc
     1561 ggttcgcaga agccggcgcg gctgagggga tcttcatgag aaagtgatgc gactgtcccg
     1621 tggtggaaac atatggtctg tccaggcccc gtttccacca cgggcagtcg taacgtcact
     1681 tgtgcaagtc cttgcattcc tggcacagcg gcttgccgcc cgtgccaggc cgtcggttct
     1741 cgcgctcgat gttgttgccg gtcttgcacc cggtgtcgtt gtggtgccga tccgacttaa
     1801 tcgaatgcca aggcgaaacg atcattgggc tctcctcctt tccgacgccg gtggtgctgt
     1861 tcagcacctg ggcaaccggc tcccgtaacc aggatacgag tcaacagtcg gatcaatcgg
     1921 atttgctcgt tgaagcaatt ggtcggagga aagtgttttc cgagacgagc aggccagatg
     1981 gattaatgcg cgcgcaaatc caattatcgc gcgctctatg tgctgtactg gctaggtcga
     2041 acagaaggga gtggtcatgg caagacctaa gaagtatctg gaacgccgcg tgacgactgc
     2101 catccgccta ccagaacgcc ttcatcgcga gcttgagtca acggctcacg agagagacac
     2161 gtcaatcaac cacctcatgg tcaaagctgc agagttctat ctccgtcatc tccctcagat
     2221 cgagccagtc gttcctgaag gagtggagag tgcaatcaac tgatccaaat ggatcatgtc
     2281 ccttgcggtt tggttcagac gtcgcggaag acagtcggca cgagcgcgaa ctaggggcgt
     2341 gctagccccc gtagacctgc agcacgacct cgcgcggctt cggctcgtcg agctctacga
     2401 gcagcagccg ctgccacctc ccgagcagcg gcgtgccgcc cgagatcggg atcgtgtgcg
     2461 acgtcccgcc catcagcatc tgcgccacgt gagcgtggcc attggcgcgc tccccgggct
     2521 gcaggttctg cgtcctgatc gcgaggtcgt cgtgcgcgta gtagatcgac ggggggaaga
     2581 gctggtcgag ccgggcggcg aggtcggcct gggcaccgtc ctcgagctcg ttgatcagca
     2641 gcgcacacgt cgtatgccgc gcgaaggcga tggcgaagcc gtcggcttcg ccgagccggg
     2701 cggcctcgct cacgaggtgc cgggtgaggt cgacgaagcc cagccgatgt cgagcctcca
     2761 ccgtcacgat cgtcgtggtc gggttgttca gtaatagctg catctcatgt cctccggtcg
     2821 ggccaggcgt ggcgcgcggt cgcgcgacag gttgaacgag ctccaggggg ccgggtcgtt
     2881 gcgcgccgct atctcgtagg cggcgcaggc gcgctcttcg agcgggaggt ccgccagcgc
     2941 gggcagcgcg tcggtgctga gcgtggacag atagatctcg tcgacctcgc cggtgcgctc
     3001 gaagcgctcg accgcgctgc gtgcgatcag ggcgtccggg ttggcgacgt tgaacgcgat
     3061 cagcccggcg acggacagcg ccacgaccgc gcgcggcagc cacgatcctc gccggaacag
     3121 gccggccagc atcaccgcga cgaagatcgc cgccagccac aggactacgg cgaagaccga
     3181 gacccggatg cgcgtgagcc cgtacgcgtc ctcgtagagg ctcatccggc gcaatgccga
     3241 cgcgaggacg acgagcgtca ggccgcacag gaggcccagc agagcgttgc ggaggcgttc
     3301 ctggtccggt ggcgcgagct tcaccgcgag cgcgatcacg ccgaggacga gggccgcgat
     3361 ccacacgagc tggaagaacc ccgcccgtgc gtactgcgcg tacgtgagcc cggcggtgtc
     3421 gagcacgtgt ccgtgccccc cgaacaggac cgcgacctgg acgacgacga acgcggcgaa
     3481 cagcacgttc agagcgagca gcgggccggc ccattcgagc gtcgtcgcga ggcgccaccg
     3541 cgccccctcg ggcgaaggct cctcctgcgg cctctgcgcc atgaggatca gccctccggc
     3601 ccccgccgcg accgcgagca cgacgaagat gcgcgccggc aagaggtcga ggccgacgtc
     3661 cggcacgagc acgtcctcgg ctatctgggc gaacgcggcg tcggcgctcg cgaacaggcc
     3721 gccgaacgtg acgagcaaca ggaacgacgt gagggcggcc cggcccgccg gcgcgagcca
     3781 cggcaccgag cgctctcgcg ggatcgaccg gtagagacgg cggcagggac gcggaaggcg
     3841 tcgccggccg cgcagagagg cgccgcgagc accgtgaccc acgtgcgggc gccggcgatc
     3901 gcgagagccg cgcagacggc agccgcggcg acgtcgaccg cgatcaccca gcgcgcgtcg
     3961 tagatcgtcg ccatccccaa aagcaccagc gcggcggcgg cgaaggcaac ggactcgaga
     4021 ctcagaggtc tcggccgcgc gatgccgacg gcagccgcga ccgcgatccc ggtgacgagc
     4081 aacccgaccc cgggaggccc cgcgggtacg agcgcgccgc cgacgatgga cgcggccccg
     4141 atttcggccc agacgcgcag cggcggcacg acaggcgctt cgtcgatcac gccgcggccc
     4201 ccggcagcga cacgaccatg cggcagccgc gcggctcgtt cgcctcgccc gcgatccgcc
     4261 cgccgtgcag gtcgacgatc cactacgcga tcgacagtcc gaggccggtg cctccggcgg
     4321 acgacgaccg tgcgtggtcg gcgcggtaga agcgctcgaa cacgcgggcc gcgtcctcgg
     4381 cggggatacc cggcccctcg tcggcgacct cgatgcggac gccgtcgggc tcgtgccggg
     4441 ccgcgacgac gacgcggccc tgtgg
//
LOCUS       DASQDO010000557         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_180376, whole genome shotgun sequence.
ACCESSION   DASQDO010000557 DASQDO010000000
VERSION     DASQDO010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180376"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2435)
                     /locus_tag="VEV43_15290"
     CDS             complement(<1..2435)
                     /locus_tag="VEV43_15290"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYP24930.1"
                     /translation="MRTASSKTFRNDDGTFTTRVFSGPVHFREDGAWKPIDTDLVPAD
                     APGFAYRNAAAGFRALFKEELGEGFMRFDAGGRTFDFTLDAAARGASSRAKSGVAYRG
                     AFPGVDLSYDIVPDGVKETLVLDSAAVPSHYRFYLETADAESLIVDELPNGAWAFRER
                     RAEEPLFVLDPPYAIDAADEEFELPPATAPEPEPTPSPSPSPSPSPSPSAPEETPSAD
                     ETASPEPPATTPSPDVTTKPEDVPTSEPATPQQRLPATKLAERTRPEDTHAEMKVTRV
                     GGRFAVDLSVDAAWLHDRDRAFPVLVDPSITIKPSLEDANFNASCPTCTPSLRDRNYI
                     GTTDTNAWRAALTFDLGDIPASSAQISSAQLKLYYGHCIPVGTGTCRDRSHQLDVHRM
                     TSAWTTSSTTQSLGFEATPLANYTLATNAEHTWMNWNVTTTLKNWLSGTQTNHGLLVK
                     RSVESTLGHSGPRPPGRRYTEEASLRPALEVTWTADAVTLAEPDTLHSNGAELDWTEY
                     AGTSAFTSYEVHRSATARFTPSSSTLLATIGSKATTSYRDTTASPSKAFSYKVVTNSN
                     VSNERTVTLPAAGQATKVLQPGPAKTKAAYLTHDTGYTVCDNNGARDKLYIGSESTYV
                     RRPVLEFDLQEIPAAATVTSATMSLWHGSTIYFAPTVNAHRVTSAWQEGTGNSSCTRN
                     GATWYSATGTVPWVTQGGDFDSTVSASVTHVSGDTAGWDNFNVTTLVGDWLSGKAPNH
                     GVVLKLSDETLAAGRAFSYWSDDFAVAPTLQPKLVLQYSEPVNAQGPSVTVSSHANNA
                     NVSGSVSLAAAAT"
BASE COUNT          410 a          923 c         1050 g          468 t
ORIGIN      
        1 tccgtggcgg ccgccgccag cgacaccgat ccgctgacgt tcgcgttgtt cgcgtgcgag
       61 gagaccgtca ccgacggccc ctgcgcgttg accggctcgc tgtattgcag gacgagcttc
      121 ggctgcagcg tgggcgcgac cgcgaagtcg tcggaccaat aggagaaagc gcggccggcc
      181 gcaagcgtct cgtccgagag cttgaggacg acgccgtggt tgggcgcctt cccgctcagc
      241 cagtcaccga cgagcgtggt gacgttgaag ttgtcccagc cggcggtgtc gccgctcacg
      301 tgcgtcaccg acgccgagac ggtcgagtcg aagtcgcctc cctgcgtcac ccaagggacg
      361 gtcccggtcg cgctgtacca ggtagctccg ttccgggtgc aggagctgtt ccccgtgccc
      421 tcctgccagg cggacgtgac gcgatgagcg ttcacggtcg gggcgaagta gatcgtgctc
      481 ccgtgccaca gcgacatcgt ggcgctcgtc accgtcgcgg ccgcggggat ctcctggagg
      541 tcgaactcga ggacgggccg gcggacgtac gtcgactcgc taccgatgta cagcttgtcg
      601 cgcgcgccgt tgttgtcgca gacggtgtag ccggtgtcgt gcgtgaggta ggcggccttc
      661 gtcttcgccg gccccggctg caggaccttc gtcgcctggc ccgccgccgg cagggtcacc
      721 gtgcgctcgt tcgagacgtt gctgttcgtg acgaccttgt acgagaacgc cttcgacgga
      781 gacgcggtcg tgtcgcggta ggacgtcgtc gccttgctcc cgatcgtggc gaggagcgtc
      841 gaggaagaag gcgtgaaccg cgccgtagcc gagcgatgga cctcgtagct cgtgaacgcc
      901 gacgtcccgg cgtactccgt ccagtcgagc tcggcgccgt tcgagtggag cgtgtcgggc
      961 tccgccagcg tgaccgcgtc ggcggtccac gtcacctcga gcgcgggacg gagtgaagcc
     1021 tcctcggtgt agcggcggcc ggggggccgc ggcccgctgt ggcccagcgt ggactcgacg
     1081 cttcgtttca cgagcaagcc gtggttcgtc tgcgtcccgg acagccagtt cttcagcgtc
     1141 gtcgtgacgt tccagttcat ccacgtgtgc tcggcgttgg tcgcgagcgt gtagttggcg
     1201 agcggtgtcg cttcgaaccc gaggctctgc gtcgtcgagc tcgtcgtcca cgccgacgtc
     1261 atgcggtgga cgtcgagctg gtggcttcgg tcacgacacg tgccggtgcc caccgggatg
     1321 cagtggccgt agtagagctt cagctgtgcg ctcgagatct gcgcggagct agcggggatg
     1381 tcgccgaggt cgaacgtgag ggccgctcgc cacgcgttcg tgtcggtggt tccgatgtag
     1441 ttgcggtccc tcaagctcgg cgtgcacgtc ggacagctgg cgttgaagtt cgcgtcctcc
     1501 aggctcggct tgatcgtgat cgacggatcg accaggacgg ggaacgcgcg gtcgcggtcg
     1561 tggagccacg cggcgtcgac ggagaggtcg acggcgaacc ggccgccgac ccgcgtgacc
     1621 ttcatctcgg cgtgggtgtc ctcgggacgc gtccgctcgg cgagcttcgt cgcggggagg
     1681 cgttgctgcg gcgtcgcggg ctcggacgtg gggacgtcct ccggcttcgt ggtcacgtcc
     1741 ggcgacggcg tggtcgcggg cggctccggc gacgcggtct cgtcggccga cggcgtctcc
     1801 tccggcgcgg acggagaggg cgagggcgac ggggacggcg agggggaggg cgtcggctcg
     1861 ggctcggggg ccgtggccgg cgggagctcg aactcctcgt cggcggcgtc gatcgcgtac
     1921 ggcggatcga ggacgaacag aggctcctcg gccctccgct cccggaaggc ccaggcgccg
     1981 ttcggcagct cgtccacgat caacgactcc gcgtccgcgg tctccagata gaagcggtag
     2041 tgggacggga ccgcggccga gtccaggacc agcgtctcct tcacgccgtc cggcacgatg
     2101 tcgtagctga ggtcgacacc ggggaaggcg ccgcggtagg cgacgccgga cttggcccgg
     2161 ctggaggcgc cacgcgctgc ggcgtccagc gtgaagtcga acgtccgccc gcccgcgtcg
     2221 aagcgcatga agccctcgcc gagctcttcc ttgaacagcg ctcggaaacc ggccgcggcg
     2281 ttgcgatagg cgaagccggg agcgtccgcg gggacgaggt cggtgtcgat cggcttccag
     2341 gccccgtcct cgcggaagtg gacggggccg ctgaacaccc tcgtcgtgaa ggtcccgtcg
     2401 tcgttgcgga acgtcttcga gctcgcggtt cgcaaggatt cgacctcgcg accgccgtct
     2461 ctggcggccg gctgtgcaga gacggccgcg gccgtcggat acagcagtcc gccgagtacg
     2521 gcgatggcga cgcccagaac gagtacgcgc gtgcgacgaa acgacatgcc cactacgaac
     2581 acccccacaa tcgatccgac tgataccggg tgttcgcagg ccgtccgccc aataaaaacc
     2641 cgtgacgtcg aacgttaggg aggacgcgct cgcggggcca tagggatcac gggtgatctt
     2701 ggttgccggg ccgggccaat tcgagccggg gttagaacca cggagcgcga agaaccaatg
     2761 acgcgcgcgg actagacacg acggcttctc cgaaccgaac gccggcgtcg tcggtaggtc
     2821 gagtcgatcg cgccggcgag gagctcacta g
//
LOCUS       DASQDO010000558         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1158778, whole genome shotgun sequence.
ACCESSION   DASQDO010000558 DASQDO010000000
VERSION     DASQDO010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158778"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VEV43_15295"
     CDS             <1..773
                     /locus_tag="VEV43_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24931.1"
                     /translation="AENLAVQGDDNRKDDIFVRDVLEGKTNRANIRTETLKQANRDSS
                     DPSISAEGEYVAFASSADTLSKGDTNNSPDVFRYNRKLHATVRASLAFGGAGSNAAGP
                     SSQPSISDDGKRIAFVSKAVNLVEDDATNDPDVFVYDFEAKETTLVTNEVADGPAGDT
                     DNPVISGNGRFVAFDSTASNLVEGDRNGVRDVFVYDLVEGEMVRVSVDEGGGEGSEAS
                     FDPSISSDGSFVTFTSEGRLVSTDTNGVPDVYLRGPLY"
     gene            complement(767..1168)
                     /locus_tag="VEV43_15300"
     CDS             complement(767..1168)
                     /locus_tag="VEV43_15300"
                     /inference="COORDINATES: protein motif:HMM:NF018271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24932.1"
                     /translation="MRPLARLPIYATAGFAFEVAFSALHDAARGRRVRFRTSPWMLTV
                     YALLLPLYEPVHDGLRGKPLPVRATVYGLGFLAVEYASGTVFRRALGEAPWDYTYAKR
                     HVNGLIRPDYFFLWALAGLLAEHLHDELTGQ"
     gene            1237..1923
                     /locus_tag="VEV43_15305"
     CDS             1237..1923
                     /locus_tag="VEV43_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HYP24933.1"
                     /translation="MSLAPRIRVVSTLSVVVVVALVAVATSWNTGEMSPAACRAAKKA
                     RAVMRNVDGEKIGVVEFWNDAACVTRVVAKIGSFNLQGDETGLTEGFHGFHIHSTGEC
                     DRAAVDGEGQTSPFLSAGGHWNPDERNHGNHRGDLPPLLATTSGLARANVLTDRFTIN
                     RLFDDDGSAVIIHADPDNLAHVPATDGDGNERYHSHVDDATGPDAATRATGDGGSRFA
                     CGIVVKIQRS"
     gene            1975..>2179
                     /locus_tag="VEV43_15310"
     CDS             1975..>2179
                     /locus_tag="VEV43_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24934.1"
                     /translation="MRTVVLVGAGATLAEALPARPPREQLPPLDATFFELCRLARLPG
                     RETVQTYMEDHFGIDPFAGGHTME"
BASE COUNT          376 a          715 c          786 g          302 t
ORIGIN      
        1 cggccgagaa cctcgccgtg cagggcgacg acaaccgcaa ggacgacatc ttcgtgcgcg
       61 acgtgctgga ggggaagacg aaccgcgcga acatccggac cgagacgctc aagcaggcga
      121 accgggacag ctccgacccg agcatctccg ccgagggcga gtacgtcgcc ttcgcctcgt
      181 cggcggacac gctgtcgaaa ggcgacacga acaactcccc agacgtcttc cgctacaacc
      241 gcaagctgca cgcgaccgtg cgtgcaagcc tcgcgttcgg cggagcggga tcgaacgcag
      301 ccgggccgag ctcgcagccg tcgatctccg acgacggcaa gaggatcgcg ttcgtctcga
      361 aggccgtgaa cctcgtcgag gacgacgcga cgaacgaccc ggacgtcttc gtctacgact
      421 tcgaagccaa ggagacgacg ctggtgacga acgaggtggc ggacggcccc gccggcgaca
      481 cggacaaccc cgtgatctcg ggcaacggcc ggttcgtggc attcgactcg acggcttcga
      541 acctcgtaga aggcgatcgc aacggcgtcc gcgacgtctt cgtctacgac ctcgtcgaag
      601 gcgagatggt acgggtcagc gtcgacgagg gcggcggcga ggggtccgag gcctcgttcg
      661 acccgtcgat ctcgtcggac ggctcgttcg tgacgttcac ctccgagggg cggctcgtga
      721 gcacggacac gaacggcgtc ccggacgtgt acctgcgcgg ccccctctac tgaccggtca
      781 gctcgtcgtg gaggtgttcc gccaggaggc cggcgagggc ccacaggaag aagtagtcgg
      841 gccggatcaa gccgttcacg tggcgcttcg cgtaggtgta gtcccacggt gcctcgccga
      901 gagctctcct gaacacggtc ccgctcgcgt actcgacggc taggaagccg aggccgtaga
      961 cggtcgcccg gaccgggagc ggcttgccgc ggaggccgtc gtggacgggc tcgtagagcg
     1021 gcagcagcag tgcgtagacc gtgagcatcc acgggctcgt gcggaaccgg acccggcggc
     1081 cgcgggctgc gtcgtggagc gcgctgaagg cgacttcgaa cgcgaagccg gcggttgcgt
     1141 atatcgggag ccgcgcgagt ggtctcaccg gcgggattct gccggaaccg ccggggtatg
     1201 gggtgctgga gccaagtcgc ccgagggagg ccctgcatgt cactagctcc gcggatccgt
     1261 gtggtcagca cgctgtcggt cgtcgtcgtg gtcgcgctgg tcgccgtcgc gacgtcctgg
     1321 aacacgggcg agatgtcgcc ggccgcgtgc cgggcggcga agaaggcccg ggccgtcatg
     1381 cgcaacgtgg acggcgagaa gatcggcgtc gtcgagttct ggaacgacgc ggcctgcgtg
     1441 acgcgggtcg tcgcgaagat cggctcgttc aacctccagg gcgacgagac cgggctgacc
     1501 gagggcttcc acggcttcca catccactcg acgggcgagt gcgaccgcgc ggccgtcgac
     1561 ggcgagggtc agacgtcgcc gttcctcagc gccggggggc actggaaccc ggacgagcgc
     1621 aaccacggca accaccgggg cgacctgccg ccgctgctgg cgacgacctc cgggctcgcc
     1681 cgggcgaacg tcctgacgga ccggttcacg atcaaccgtc tcttcgacga cgacgggagc
     1741 gcggtgataa tccacgccga cccggacaac ctcgcgcacg tcccggcgac ggacggcgac
     1801 gggaacgagc ggtaccactc gcacgtagac gacgcgacgg ggccggacgc ggcgacgcga
     1861 gccacaggcg acggcgggag ccgcttcgcg tgcgggatag tggtgaagat ccagaggtcc
     1921 taggggggcc ggcagcgccc agaactgtca taggggagtt ggagactccc tcacatgagg
     1981 actgtcgtac ttgtgggggc gggagccacc cttgccgagg cgctaccggc gcgcccgcct
     2041 cgtgagcaac tgccgcccct cgatgccacc ttcttcgagc tttgccggct ggcgcgctta
     2101 cccgggcgcg agaccgttca gacgtacatg gaggaccact tcggaattga cccgttcgcg
     2161 ggcggtcaca cgatggagg
//
LOCUS       DASQDO010000559        15156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1158878, whole genome shotgun sequence.
ACCESSION   DASQDO010000559 DASQDO010000000
VERSION     DASQDO010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158878"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2673
                     /locus_tag="VEV43_15315"
     CDS             <1..2673
                     /locus_tag="VEV43_15315"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYP24935.1"
                     /translation="NDPDNGASFDTPDITCNTGTDGNCPVTVTQLEGSTGATTICFWI
                     GANGGTLCATEPVDEATAQNGSDTANDAADAVTKTWEARSAATGGLDVEPETDTNNLG
                     ENHTLTATVYDQFGDPFQGNTQVRFEFFAGSPSDGTDGNTTATPDRTCTTNNSATCSV
                     TYTQNTTAGTDLICAWTNAGPAVTGTNTNGTCGGEGLNDADDAAGQADEPAPAGDDVD
                     VVQKVWQNPNAPSRVDCTPEIDNNPTGTAHTVTCVTTNSFGNVTAGTRVDVEATGPGD
                     PDAANTPLTPDFTCTTNAQGSCTFTHGPGGTGTTTTPGTTVYRAWIDADNNNATNEAD
                     TTEGRDEAATAGATPEPDDTDVVNKTWTGPPATISVTPASDTASVGTCNAFTFTVVDA
                     AGNPVTGVRLDVEQRHATAADNTANNEPAVAFCLPAGAANTSAVDTDSGDLRPPSEAP
                     DNAGTAGGETTGTTNAAGQITVGVTVTPANGSNGTGNVSVQAFYETADNDDRDSNEPQ
                     ATATKTWIVPEGRTIDCEPENATNATGVTQTVICVVRDRFGEPVPNEGVTFTTSGPGT
                     LTSATTVTTDASGRAQVTATSLQPGNQTVTGTITDDVEGTEPGHVDDCDRAANDPAGS
                     PAGICADSVTLTWTQATPFSVTVSPEEAWNKPGTSHTFVATVRDQAGNPVAGVPVTWT
                     TAGAGTFSSAETSTNASGQATAVATSTARGDQNVTVSTGTCGTGGDCSAAAVKHWGPD
                     FCDIYGTEGADRLTGTAGDDVICAFDGNDVVNGGGGDDLILGHGGNDRISGGDGRDTV
                     QAGSGRDFVRGGSGLDFLRGGRGSDTLRGASGGDFLSGGSGNDDLAGGSGADTLIGRG
                     GNDRLNGGRGRDGCNGGRGRDVVRKCELRVTVTR"
     gene            2768..4009
                     /locus_tag="VEV43_15320"
     CDS             2768..4009
                     /locus_tag="VEV43_15320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HYP24936.1"
                     /translation="MSRRMLVALLVAFAATRALTGYLADHPEHYGPPDSKVTGDSANY
                     EFWAGQILTFHLNEYSEFGMEYPPGSVPFIVAPGIEKPEDDSYRTRFVLLMIAVDLAG
                     LLALIAIGRRWGSPLGAWVWVAVVPLLGPISYNRLDLVPAVATILAVERFSARSWFGG
                     GGWLGYGAIAKIYPALFLPVVFAAARRKQILTGAALVVALFLLPYLGVLDDVLRNVLG
                     YHTDRGIQIESLWGFVLLFASKLGHAIGINFTFGALHVDSSIAPALELLSQALSLGFV
                     ALGVWLAHRFRTSGRDVSGPELGGILFMTMAGTMAVGSVFSPQFMLWLGALAAAALCD
                     RATWLRIPGLAVLPAALMTQFIYPFFYGRLVAVEVPALVLLASRNFLVLFVAVEAFLA
                     LRGRAAAAPMAAAAAEPEPAA"
     gene            complement(4006..4191)
                     /locus_tag="VEV43_15325"
     CDS             complement(4006..4191)
                     /locus_tag="VEV43_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HYP24937.1"
                     /translation="MADRGEQVEGKAKEVGGKLTGNEKLESEGRTQKATEDTKEKVQE
                     GVDKVKGAVQGLKDAAR"
     gene            4479..4763
                     /locus_tag="VEV43_15330"
     CDS             4479..4763
                     /locus_tag="VEV43_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYP24938.1"
                     /translation="MSLLDTATEKLDWQEEAACRPYDNLTFFGEEGESELEKQGRESR
                     AKAVCRTCPVAEPCLEFAMETNQKYGIWGGLTDKERASLKRRRARARRAS"
     gene            4934..7327
                     /locus_tag="VEV43_15335"
     CDS             4934..7327
                     /locus_tag="VEV43_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24939.1"
                     /translation="MARTGKWPARIALAVSAAMIATLFTVINASGQSGGEALPTVGIE
                     VQSASNSQVTYAIVVRNQGSAQSTNVRVRSAVPTNTTFESSTPPPSATTSPSPGSPSC
                     DNEGNREDAGLNCEWTLGTVPAGETRQITAVYNLNQSNVATYNVSISATVTDAEGHSN
                     SDTDASLSRARQNIDHDTWVNDDEPPNWNHGECPHLRVHQNNRTTSYLQANTLPTEST
                     IERVWGALLQAQVQSTSYTAGSPGSIGAHRVTSGEWTEGTGDDAGTQNCPGEASTTTT
                     DARTGAEPQSAQNATSTSPITGFPQFASWPVTSDLDDATERVAANNNGWELKDVIGTG
                     ENFVRFHSSEATAEQQPRLFLVYTTQEAGSCVDADPETDTNPFGTEHIMTAFVTDGDP
                     VSNTGGDACNGGPAVDSVIWEIEDDTPDAYFSSQEGQPITKTIQNQDAQPDTITTVAE
                     DGTTSVGMRQDVPEAGENRVEARIVDSSQDPTDVEDQSDPDPAGTNRPACDIPMNPTE
                     ADCDGENAEHDDVRKTWNASGASGSTGTGGTTAGTTSPSRTTSSPSGTASTSPSGTAS
                     TSPSGTTPSGTTPSGTSSTTATSSASSSSPAQSARTISLFASSNEVVYPAQVTLSGTI
                     TSADNDCDNAGEFVRIDRRILGQTSYTLFETRSTDADGRFEVSFSATQSAEYTATATA
                     HDQCSEATSSPQTVTVKVKVTARSGRRSVERGQTVGIVGRVKPDHDGTTVMLQRRKGS
                     RWVTIARKDLNVRSRFRFTVDANWRGRRVFRALWRAQDQEHETNNSRRVVIRTRRPS"
     gene            7538..11185
                     /locus_tag="VEV43_15340"
     CDS             7538..11185
                     /locus_tag="VEV43_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYP24940.1"
                     /translation="MISRIKKSIELSAGDRRVAARMTLGFVAAVLCLMLALAGTSLGL
                     TGAFVVAQPSPSGSASEPAGESGSIFLLNPSPAYDPGADFVEDPVLGSGTPPPILTYP
                     KISDKFDGRDNAYHIVAVVKDPPPTALVEAYWLPETGNELTIGEMTPVQGAPNTYEIF
                     WEIPPSVTNADLGTLTARLYRDTPSGFEQVSEHSVEARMMTGPIIYSGGNAASAAETV
                     ELTWPSQNGPLGFYKGAAAGTRWKTVVEGTASVATPRDPVNNPGEHGSNGTNKVVVFY
                     SKTKPGLEPEFVNCGETTLDGADDRGFQTWSMECPLAEGDRPSQLTSLAAVAMEDQTD
                     GDESAYSQEAADVHVIQPYMQRVEDMKIELTSQVTTDQTVRRHRVAGGTTSTTACLAY
                     RIRVTDPLDRTVEGANVDVHVKGPGDGLQFGTDASSGGNNSSSSGKQKPQKAHSSELS
                     KNCDTPANTSGEQGDHNVPGGDDTKHIESTLGTGESGGGSAGRGGWVFHLFSQNQGDT
                     EITAWIDDEPIATDSQPREADDDILEPTEKSATNFAQWLPAAPAVTIDPQGATAAAGE
                     CQRFIVRVRGGARAVKGANVDVHATGPSNDLDFCDPGDGSTLNAPQQGTGHNAEDDRE
                     VAHAGEPPVAQHTEGQTNDAGNLIVGITSPVAGDTTVTAWYDGGEAPFDNDNQDSGEA
                     SATATTNWIVETGDAAISFLNPSGYGGAGSNVANTRDVDDAFHLVARVSTLQPVGVEF
                     FYRSGTDPLVKIADGTRVGETDTHEAYWPVNVEDGTYTLVARIRDTNITAEQSVTVRN
                     EDDPFDPTVPAVETLEIVSPPDGARATFVREKLTIRGVASAGAEGVYAYYTKGGPLTT
                     PAAAAWTQCGTTSLATGSAPQEFTVECTLAGSDQPALVTGVAAVAYNCFEGVCQVEDV
                     DARESHSGDAHRVIGTEANPLLVVEPAEAAAPIDTCQKFVVTLQDQTRQPIPGQNLDA
                     HLVGPGGGGNFCAPEDGTGTPRRAPNDGGHLADGDEVDEGYHEEGGTTTRHTEGQTTG
                     NGRFVFGIESATQGDSQVIVWLDDNDNDAQDQGETVDTSIMHWQGSTACDITGTSGAD
                     ELQGTDASETICGFGGNDTIRGGGGDDVIGGGAGNDRLRGNAGSDRVRGGAGRDRVFG
                     GGGGDRLSGGGGGDVVNGHTANDRLRGNRGNDRLKGGRGRDNCGGGAGRDRLRGCETG
                     ARTFAARTRPI"
     gene            11218..13194
                     /locus_tag="VEV43_15345"
     CDS             11218..13194
                     /locus_tag="VEV43_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24941.1"
                     /translation="MKKTTFLLTALIASLFPASSAIAAPTIEFLNPSGYLSTPPEISD
                     APDADGNVHLVAWARDIPANALVEFEIQPTGQNAATFTAERVGSDTWEAFVPIPDTYP
                     DGATYTLRARLYAGVPGDAEEVDNDSMTVEINQSPVPPPSGESVEITYPDNGGAMGVF
                     TPKGKRAVTVLDYVASEGTEQVRAFYTLSDPGADPDWEDTQCGSSAPGNEGFGKVRCT
                     LAEGHNPLEVSAVALVSNRAAPAPPTPPNPTLDTSGDAHRVFPYAQQPRSVEVSGGTG
                     GIVLQSCHTLTATVEDQNSRPIPVANVDVHADGPEDQLYFGTRTDGAEPGRTDGFQAP
                     DVAHVSKENAKNCTNNNDLGQQGDHNSPGRDDAKHIESTDGTSNTGTFRFALRSDFAG
                     GTFVQVWADVDDDDSPDLNEASGGTQLGWGGPAPDPVQEIFVTPTSANGSNGSCVELE
                     VLARRGGTPFNAANVDIHVRGPEPAPTFCPVAGGGTSRPPESGGHSNSTHEDGTLHAE
                     GETDASGRFVFGVSSAASGTTQVQVWIDRDDDDTLNAEPNKTATITWQPAGDRSISLT
                     SSKSRVNRGRRVRLSGRIEGDVACSAGQSVDIQAKRVRGGAFGTVKTVTTQADGSYAT
                     RVKMFKARRFRAVAQAGDPCSFARSNTVTVRVNR"
     gene            13226..14179
                     /locus_tag="VEV43_15350"
     CDS             13226..14179
                     /locus_tag="VEV43_15350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HYP24942.1"
                     /translation="MIEYGYKLSSEEQSPNDLVRYAQRAEDAGFTFAAISDHFHPWTS
                     KQGESPFVWNVIGAIAHATRELKLGTAVTCPTVRVHPAIVAHAAATSAAMMPGRFWLG
                     VGSGENLNEHVTGQRWPQTRIRQDMMVEAIEVIRTLFEGGMRSHHGRYFTVENARLYT
                     LEEAPPIYVAASGDRAAKIAGEHGDGLICVAPSDETVKRFEDAGGSGKPKYAEVNVCW
                     APTEEQAKQTALEWWPVAGLGGTLMQELALPSHFEAAAEPLDADKVSETLAVGPDPEV
                     HVENIRKFAEAGFTHLWIHQIGPDQDGFFDFYEKQVLPKVR"
     gene            complement(14176..15021)
                     /locus_tag="VEV43_15355"
     CDS             complement(14176..15021)
                     /locus_tag="VEV43_15355"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYP24943.1"
                     /translation="MTSTGSGVPPPPPPPSSFLDSGRFSILCEIQPPRRPDIEIVRDQ
                     IHVLKPVVDAFLIPDNHLGRATVSSVSVAHEVAYMGGLSIACMNARDRNVLGFRRDLL
                     TAAAYGVDHFLFVYGDSPKEGRRTEDLTVRGMIDEVRSFGTGPLFQGYPDFRIGTVVR
                     SGRDLLWRSRADFIFVQITYATSNLLAWRRSLDYEGRMYAGVLVLSSSRMAQRVNAAL
                     PDVHIPRRIVDKLDDDPEMGIEIACDQIEELRESGGFDGVHLIPVGRYREVARRLAER
                     DGKLC"
BASE COUNT         2575 a         5418 c         5250 g         1913 t
ORIGIN      
        1 aacgacccgg acaacggcgc gtcgttcgac acccccgaca taacgtgcaa caccggcacc
       61 gacgggaact gcccggtcac ggtcacccag ctcgagggtt cgaccggcgc caccaccatc
      121 tgcttctgga tcggcgcgaa cggcgggact ctgtgcgcga ccgagccggt cgacgaggcg
      181 accgcgcaga acgggtccga caccgcgaac gacgcggcgg acgccgtgac gaagacgtgg
      241 gaggcgcggt ccgccgcgac gggcgggctc gacgtcgagc ccgagaccga cacgaacaac
      301 ctcggcgaga accacacgct caccgcgacg gtctacgacc agttcggcga cccgttccag
      361 ggcaacacgc aggtccggtt cgagttcttc gccggctcgc cctcagacgg gaccgacggg
      421 aacaccacgg cgacgcccga ccggacgtgc acgacgaaca actcggcgac gtgctcggtg
      481 acctacacgc agaatacgac cgcggggacc gacctcatct gcgcgtggac gaacgccggg
      541 cccgccgtga cgggcaccaa caccaacggg acgtgcggcg gtgaagggct gaacgacgcc
      601 gacgacgccg ccggccaggc cgacgagccc gctcccgcgg gagacgacgt ggacgtggtg
      661 cagaaggtgt ggcagaaccc gaacgcaccg tcccgggtcg actgcacgcc cgagatcgac
      721 aacaacccga ccgggaccgc gcacacggtc acgtgcgtca cgacgaactc gttcgggaac
      781 gtcaccgccg gcacccgcgt cgacgtcgaa gcgaccggcc cgggcgatcc cgacgccgcg
      841 aacacgccgc tgacgccgga cttcacgtgc acgacgaacg cgcagggatc gtgcacgttc
      901 acgcacggtc cgggcggcac gggcacgacg accacgccgg ggacgaccgt ctaccgcgcg
      961 tggatcgacg ccgacaacaa caacgcgacg aacgaggccg acacgacgga ggggcgtgac
     1021 gaggccgcga ccgcgggggc aacgccggag cccgacgaca ccgacgtcgt caacaagacg
     1081 tggacgggcc cgcctgcgac gatctcggtc acccctgctt ccgacaccgc gtcggtgggg
     1141 acctgcaacg ccttcacgtt cacggtggtg gacgcggcgg gcaaccccgt gaccggcgtg
     1201 cgcctcgacg tcgagcagcg gcacgcgacg gcggccgaca acaccgcgaa caacgagccg
     1261 gccgtcgcgt tctgccttcc cgccggcgcg gccaacacca gcgcggtcga caccgactcg
     1321 ggcgacctcc ggcccccgtc cgaggccccg gacaacgcgg ggaccgccgg aggcgagacg
     1381 accggcacga cgaacgccgc cggccagatc accgtaggcg tcaccgtcac gccggcgaac
     1441 ggatcgaacg ggaccggcaa cgtgtcggtg caggcgttct acgagaccgc cgacaacgac
     1501 gaccgcgact cgaacgagcc ccaggccacc gcgacgaaga cgtggatcgt gcccgagggc
     1561 cggacgatcg actgcgagcc ggagaacgcc acgaacgcca ccggcgtcac ccagacggtc
     1621 atctgcgtcg tccgcgaccg cttcggcgag cccgtgccca acgagggcgt gacgttcacg
     1681 acgagcggtc cgggcacgct gaccagcgct acgacggtca cgaccgacgc gtcgggccgc
     1741 gcccaggtca cggcgacgtc gttgcagccg ggcaaccaga cggtcaccgg gacgatcacg
     1801 gacgacgtcg aaggcaccga gcccggacac gtggatgact gcgaccgggc cgccaacgac
     1861 cctgcaggct cacccgcggg gatctgcgcc gactcggtca cgctgacgtg gacgcaggcc
     1921 acgccgttct cggtgacggt ctcgcccgag gaggcgtgga acaagccggg gacgtcgcac
     1981 acgttcgtgg cgaccgtgcg cgaccaggcc ggcaaccccg tcgcgggcgt ccccgtgacg
     2041 tggaccacgg cgggcgccgg gaccttctcg tcggccgaga cctcgacgaa cgcgtcgggc
     2101 caggccacgg ccgtcgcgac gtcgaccgcc cgcggcgacc agaacgtcac ggtgtcgacc
     2161 gggacgtgcg ggacgggtgg cgattgctcg gccgccgcgg tgaagcactg ggggccggac
     2221 ttctgcgaca tctacggcac cgaaggggcc gaccgcctga ccgggaccgc cggtgacgac
     2281 gtcatctgcg ccttcgacgg caacgacgtc gtgaacggcg gcggaggcga cgacctgatc
     2341 ctcggccacg gaggcaacga caggatctcc ggcggcgacg gacgggacac cgtccaggcc
     2401 ggttcgggcc gtgacttcgt caggggcgga tcgggcctcg acttcctccg cggaggccgg
     2461 ggcagcgaca ccctgcgcgg ggcgagcggc ggtgacttcc tcagcggagg gagcggcaac
     2521 gacgacctcg ccggcgggag tggcgccgac acgctcatcg gccgcggcgg caacgaccgc
     2581 ctcaacggcg gccgcgggcg cgacggctgc aacggcggac gtggccgcga cgtcgttcgc
     2641 aagtgcgagc tgcgcgtgac ggtcacgaga tagacgcaag gcaaggcaag ggggccggtc
     2701 cttcggggcc ggccctctgc gcgggacgcc cgaaaggaaa acgtccgacg cctcatactt
     2761 ctaggccatg agccgccgga tgctggtcgc cctgctcgtc gcgttcgccg cgacgcgggc
     2821 gctcacgggc tacctcgcgg accatcccga gcactacggc ccccccgaca gcaaggtcac
     2881 cggcgactcg gccaactacg agttctgggc cgggcagatc ctgacgttcc acctcaacga
     2941 gtactccgag ttcggcatgg agtacccgcc gggctcggtc ccgttcatcg tggcccccgg
     3001 gatcgagaag cccgaagacg acagctaccg cacgcgcttc gtcctgctga tgatcgccgt
     3061 agacctcgcc ggcctgctcg cgttgatcgc catcgggcgg cggtggggat caccgctcgg
     3121 cgcgtgggtg tgggtcgcgg tcgtgccgct gctggggccg atctcgtaca accggctcga
     3181 cctcgtgccc gcggtcgcaa cgatcctggc ggtcgagcgg ttctcggcgc gctcctggtt
     3241 cgggggcggc gggtggctcg gctacggcgc gatcgcgaag atctaccccg cgctgttcct
     3301 gccggtcgtg ttcgcggcgg cgcggcgcaa gcagatcctg accggcgcgg ccctcgtcgt
     3361 cgcgctgttc ctgctgccgt acctgggcgt cctcgacgac gtcctgcgca acgtcctcgg
     3421 ctaccacacc gaccgcggga tccagatcga gagcctctgg ggctttgtcc tcctgttcgc
     3481 gtcgaagctc gggcacgcga tcggcatcaa cttcaccttc ggcgcgctgc acgtcgattc
     3541 gagcatcgcc ccggccctcg agctcctgtc gcaggcgctc tcgctcgggt tcgtcgcgct
     3601 cggcgtgtgg ctggcgcacc gcttccgcac ctccggtcgg gacgtctccg ggccggagct
     3661 cggcgggatc ctgttcatga cgatggccgg gacgatggcg gtggggtcgg tgttctcgcc
     3721 gcagttcatg ctgtggctcg gggcgctcgc cgccgcggca ctgtgcgaca gggcgacgtg
     3781 gctgcgcatc cccgggctcg ccgtgctgcc ggccgcgctg atgacccagt tcatctaccc
     3841 gttcttctac gggcgcctgg tggcggtgga ggtgccggcg ctcgtgctcc tggcgagcag
     3901 gaacttcctc gtcctgttcg tcgccgtgga ggctttcctc gctctgcgag ggcgggcggc
     3961 ggccgcccct atggcggcgg cggccgcaga gcccgaaccg gccgcttagc gggccgcgtc
     4021 cttgaggccc tggacggcgc ccttgacctt gtccacgccc tcctggacct tctccttcgt
     4081 gtcctcggtc gccttctgcg tgcggccctc ggactcgagc ttctcgttgc ccgtcagctt
     4141 cccgcctact tccttggcct tgccttcgac ctgctcgccg cgatctgcca tgggtccacc
     4201 tctcctcgat cctgctcctg cttcgtcccg cgtgactacc cggggggccg aagggggaaa
     4261 ctgcggggcg aggcggctac ggtctggctt tttcgacctg cctcgtctat ggtgcggggg
     4321 tcctcgaccc ctgcccgcct tgcggccggc gagggatggt ctccagacgc ggggcctgcc
     4381 ggaatagacc gggcccaggg ctcgtttagc cattcgtccg ggaggacata gaaggtgcgc
     4441 ccgcgggcgc tgttcgagac ccgattggag ggaaccacgt gtccttgctg gacaccgcga
     4501 ccgagaagct cgactggcag gaggaggcag cctgccggcc ctacgacaat ctgacgttct
     4561 tcggtgagga gggcgagtcc gagctcgaga agcagggccg ggagagccgt gcgaaggccg
     4621 tttgccgcac ctgcccggtc gccgagccgt gcctggagtt cgcgatggag accaaccaga
     4681 agtatgggat ctggggaggt ctcaccgaca aggagcgagc ctcgctcaag cggcgccgcg
     4741 cccgcgcccg ccgcgcttcc tagcaccggc ctgacgccca tacgtcatct ccctcccggt
     4801 acctgtcgcc gggagcaacc tccgtggccg cgctcacgtt taaccttgta ggggggcatg
     4861 ccgtccatac ggcgcccccg tgacaggtgc taggttccaa caggcgtcga aggcttcgga
     4921 aggggacaac cgtatggctc gtaccggcaa gtggcccgcg cgcatcgcgc tagcggtcag
     4981 cgccgcgatg atcgcgacgc tcttcaccgt gatcaacgcg tccggacagt ccggcggtga
     5041 ggctctcccg accgttggca tcgaggtcca gtcggcctcc aacagccagg tgacgtacgc
     5101 gatcgtcgtc cggaaccagg gctccgccca gtcgacgaac gtgcgcgtgc ggagcgccgt
     5161 cccgacgaac acgaccttcg agagcagcac ccccccgccg agcgcgacca cctccccgtc
     5221 cccgggatcg ccgtcgtgtg acaacgaggg gaaccgcgag gacgccggtc tcaactgcga
     5281 atggaccctc gggaccgtcc ccgccggcga gacccgccag atcacggcgg tctacaacct
     5341 gaaccagagc aacgtcgcga cctacaacgt ctcgatcagc gccaccgtga ccgacgccga
     5401 gggccactcg aacagcgaca cggacgcttc gctgtcccgg gcgcggcaga acatcgacca
     5461 cgacacctgg gtcaacgacg acgagcctcc gaactggaac cacggcgagt gcccgcacct
     5521 ccgtgtgcac cagaacaacc gcacgacgag ctacctccag gccaacaccc tgccgacgga
     5581 gagcacgatc gagcgcgtct ggggcgccct gctccaggcc caggtccaat ccacgagcta
     5641 caccgcgggc tcgccgggct ccatcggcgc ccaccgggtc acgtccggcg agtggaccga
     5701 agggaccggc gacgacgcgg ggacccagaa ctgcccgggc gaggccagca cgacgacgac
     5761 cgacgcccgc acgggggccg agcctcagag tgctcagaac gccacgtcga cctcgcccat
     5821 cacggggttc ccccagttcg cctcgtggcc ggtgacgtcc gacctcgacg acgcgaccga
     5881 gcgtgtggcc gccaacaaca acggctggga gctcaaggac gtcatcggga ccggtgagaa
     5941 cttcgtccgg ttccactcga gcgaggcgac ggccgagcag cagccgcggc tcttcctggt
     6001 ctacacgacg caggaagcgg gctcgtgcgt ggacgccgac cccgagaccg acacgaaccc
     6061 gttcgggacc gagcacatca tgacggcctt cgtcacggac ggcgatccgg tgtccaacac
     6121 cggcggcgac gcctgcaacg gcggccccgc ggtcgacagc gtcatctggg agatcgagga
     6181 cgacacgccc gacgcgtact tctcgtcgca ggagggccag ccgatcacca agaccatcca
     6241 gaaccaggac gcccagccgg acacgatcac gacggtcgcc gaagacggga cgaccagcgt
     6301 cgggatgagg caggacgtgc cggaggccgg cgagaaccgg gtcgaggccc ggatcgtcga
     6361 cagctcacag gaccccaccg acgtggagga ccagagcgac cccgatcccg ccggcaccaa
     6421 ccgtcccgct tgtgacatcc ccatgaaccc cacggaagcc gactgcgacg gcgagaacgc
     6481 cgagcacgac gacgtgagga agacctggaa cgcatcgggt gcttcgggct ccaccggcac
     6541 gggcggaacg acggccggca cgacgtcacc gagccggacg accagctcgc cgtcggggac
     6601 cgccagcacc agcccgtcgg gcaccgccag caccagcccg tcggggacca ccccgtcggg
     6661 gaccaccccg tcggggacct cttccacgac ggcgacgtcg agcgcgagct cgtcgagccc
     6721 ggcgcagagc gcgcggacga tctcactgtt cgcctcgtcg aacgaggtcg tgtaccccgc
     6781 gcaggtcacg ctgtcgggga cgatcacgtc cgcggacaac gactgcgaca atgccggcga
     6841 gttcgtccgg atcgaccggc ggatcctcgg ccagacctcc tacacgctgt tcgagaccag
     6901 gagcaccgac gccgacggcc gcttcgaggt ctcgttctcc gcgacccaga gcgccgagta
     6961 caccgctacc gctacggcgc acgaccagtg ctcggaggcg acctcgtcgc cccagaccgt
     7021 gacggtcaag gtcaaggtca cggcgcggtc cggccggcgc tcggtcgagc gggggcagac
     7081 cgtcgggatc gtcggtcgcg tcaagcccga ccacgacggc accacggtca tgctgcagcg
     7141 gaggaagggc agccgttggg tcacgatcgc ccggaaggac ctcaacgtcc gctcccggtt
     7201 ccgcttcacg gtcgacgcca actggcgggg ccggagggtc ttccgcgcac tgtggcgggc
     7261 ccaggaccag gagcacgaga ccaacaacag ccggagggtc gtcataagga ccagacgtcc
     7321 ttcctaggaa cccatgatcg agccacggga gccggcccct cgggccggct ccttcgtgtc
     7381 gaggccttcg cggtccacgt cgcaacccaa gcgccggatc gttcgttact gcaggtagac
     7441 ccccgatacg gcatccgacc ccggaagacc tggtcgggga tacttagggg cctatcgcga
     7501 gccatagagg ggcgagacgc accccaggag gtgtgtgttg atatctagga tcaagaagtc
     7561 gatcgagctg tccgcgggcg accgccgggt agccgcgcgc atgacgctgg gcttcgtcgc
     7621 ggcggtcctg tgcctgatgc tcgcgctggc cgggacgtct ctcggcctca ccggcgcgtt
     7681 cgtcgtagct cagccgtcgc cgagcgggtc tgcctccgag ccggcgggcg agtcggggtc
     7741 gatcttcctg ctcaacccga gccccgccta cgaccccggc gcggacttcg tcgaagaccc
     7801 ggtcctcggc tccgggacgc cgcccccgat cctcacgtac cccaagatca gcgacaagtt
     7861 cgacgggcgg gacaacgcct atcacatcgt cgccgtcgtc aaggacccgc cgccgacggc
     7921 cctcgtcgag gcgtactggc tgcccgagac gggcaacgag ctgacgatcg gcgagatgac
     7981 gcccgtccag ggggcgccca acacctacga gatcttctgg gagatcccgc cgtccgtcac
     8041 caacgcggac ctcgggacgc tgaccgcgcg cctttaccgg gacacgccga gcggcttcga
     8101 gcaggtctcc gagcactccg tcgaggcccg catgatgacc ggcccgatca tctacagcgg
     8161 cgggaacgcc gcgtcggcgg ccgagaccgt cgagctgacc tggccgtcgc agaacgggcc
     8221 cctcggcttc tacaaggggg ccgcggccgg cacccgctgg aagaccgtgg tggaaggcac
     8281 cgcgagcgtc gccacgccgc gagacccggt caacaacccc ggcgagcacg gcagcaacgg
     8341 cacgaacaag gtggtcgtct tctactcgaa gaccaagcct gggctcgagc cggagttcgt
     8401 caactgcggc gagacgacgc tcgacggcgc agacgaccgc ggcttccaga cgtggtcgat
     8461 ggagtgcccg ctggccgagg gcgaccgccc ctcgcagctc acctcgctcg cggccgtggc
     8521 gatggaggac cagaccgacg gagacgagag cgcctacagc caggaggccg cggacgtcca
     8581 cgtgatccag ccctacatgc agcgggtcga ggacatgaag atcgagttga cgtctcaggt
     8641 gacgaccgac cagaccgtgc ggcgccaccg cgtggcgggc ggtacgacgt cgacgacggc
     8701 ctgtctggcc taccggatca gggtcaccga cccgctcgac cggaccgtcg agggcgcgaa
     8761 cgtcgacgtc cacgtgaagg gccccggcga cgggctccag ttcgggaccg acgcgtccag
     8821 cggaggcaac aacagctcct ccagcggcaa gcagaagccg cagaaggcgc attcgagcga
     8881 gttgagcaag aactgcgaca cccccgcgaa cacgtcgggc gaacaggggg accacaacgt
     8941 cccgggaggc gacgacacca agcacatcga gagcacgctt ggcacgggcg agtccggcgg
     9001 gggctccgcc ggccggggcg ggtgggtctt ccacctcttc tcgcagaacc agggcgacac
     9061 cgagataacg gcgtggatcg acgacgagcc catcgccacc gactcgcagc cgcgtgaggc
     9121 cgacgacgac atcctcgagc cgaccgagaa gtccgccacg aacttcgcgc agtggcttcc
     9181 cgcggccccc gcggtgacga tcgatcccca gggcgcgacg gccgcggccg gcgagtgcca
     9241 gcggttcatc gtccgggtgc ggggcggggc gcgcgccgtg aagggcgcca acgtcgacgt
     9301 ccacgcgacg ggcccgtcga acgacctcga cttctgcgac ccgggcgacg ggtcgaccct
     9361 caacgctcca cagcagggga ccgggcacaa cgccgaggac gaccgcgagg tcgcgcacgc
     9421 gggcgagccc ccggtggccc agcacaccga gggccagacg aacgacgccg ggaacctgat
     9481 cgtcggcatc acctcccccg tcgccggcga caccacggtc acggcctggt acgacggcgg
     9541 cgaggccccg ttcgacaacg acaaccagga ctccggcgag gcgagcgcca cggcgacgac
     9601 caactggatc gtcgagacgg gcgacgcggc gatcagcttc ctcaaccctt cggggtacgg
     9661 gggtgccggc tcgaacgtgg cgaacacgcg cgacgtcgac gacgccttcc acctcgtcgc
     9721 acgcgtctcg acgctccagc ccgtcggggt cgagttcttc taccgctccg gcaccgaccc
     9781 gctcgtgaag atcgcggacg ggacgcgcgt cggcgagacc gacacccacg aggcgtactg
     9841 gccggtgaac gtcgaggacg ggacctacac gctcgtcgct cgcatccgcg acaccaacat
     9901 caccgcggag caatcggtca ccgtgcgtaa cgaggacgac ccgttcgacc cgacggtccc
     9961 ggccgtcgag acgctcgaga tcgtctcgcc tccggacgga gcgcgcgcga cgttcgtgcg
    10021 agagaagctc acgatccgcg gcgtcgcgag cgcgggggcc gagggcgtct acgcgtacta
    10081 cacgaagggc ggcccgctca cgacgccggc tgcggccgcc tggacgcaat gtgggaccac
    10141 gagcctcgcc acgggctcgg caccgcagga gttcacggtc gagtgcacgc tggccgggtc
    10201 cgaccagccc gcgctcgtca cgggcgtggc ggcggtggcg tacaactgct tcgagggcgt
    10261 gtgccaggtc gaggacgtgg acgcccgcga gagccactcc ggcgacgcac accgggtgat
    10321 cggcaccgaa gcgaacccgt tgctcgtggt cgagcccgcc gaggccgcgg ccccgatcga
    10381 cacgtgtcag aagttcgtcg tcacgctcca ggaccagacg cggcagccga tcccgggcca
    10441 gaacctcgac gcccacctcg tcggccccgg tggcggcggc aacttctgtg cccccgagga
    10501 cgggaccggg acgccgcgcc gggctcccaa cgacggcggg cacctcgccg acggcgacga
    10561 ggtcgacgag ggctaccacg aagaaggcgg gacgaccacg cgccacaccg agggccagac
    10621 gaccggcaac ggccgcttcg tgttcgggat cgagtccgcg actcaagggg actcgcaggt
    10681 gatcgtctgg ctcgacgaca acgacaacga cgcgcaggac cagggcgaga ccgtggatac
    10741 gtcgatcatg cactggcagg gcagcaccgc ctgtgacatc accggcacga gcggcgcgga
    10801 cgagctccag ggcaccgacg cctccgagac catctgcggg ttcggcggca acgacacgat
    10861 ccgcggtggc ggcggcgacg acgtcatcgg cggcggcgcc gggaacgacc gtctacgcgg
    10921 caacgcgggc agcgatcgcg tccgcggcgg tgccggccgg gaccgggtct tcggcggcgg
    10981 cggcggtgac cgcctcagcg gcggtggcgg cggcgacgtc gtgaacggcc acaccgcgaa
    11041 cgaccgcctt cgcggcaacc ggggcaacga caggctgaag ggcggacgcg ggcgcgacaa
    11101 ctgtggtggt ggcgcgggac gcgaccgtct ccgcgggtgc gagaccgggg cgaggacctt
    11161 cgccgcgcgg acgcgcccga tctagcaacg accaaggcgg acccaggggg taccgaagtg
    11221 aagaagacga ctttcttgct gacggcgctg atcgcgtcgc tgttcccggc gtcgagcgcg
    11281 atcgccgcgc cgacgatcga gttcctcaac ccgtcgggct acctgtccac tccgcccgag
    11341 atctccgacg cgcccgacgc cgacggcaac gtgcacctcg tcgcgtgggc ccgcgacatc
    11401 ccggcgaacg cgctcgtcga gttcgagatc cagccgaccg gccagaacgc cgcgaccttc
    11461 acggcggagc gtgtcgggag tgacacgtgg gaggcattcg tgccgatccc cgacacgtac
    11521 ccggacggcg ccacctacac gctgcgtgcc cgcctgtatg cgggggtccc cggcgacgcc
    11581 gaggaggtcg acaacgactc gatgacggtc gagatcaacc agtcgccggt tcctccgccg
    11641 tccggcgaga gcgtcgagat cacctacccc gacaacgggg gtgcgatggg cgtgttcacg
    11701 ccgaagggca agcgagcggt gaccgtcctc gactacgtcg ccagcgaggg gaccgaacag
    11761 gtccgcgcct tctacacgct ctccgacccc ggggccgatc ccgactggga ggacacgcag
    11821 tgcggctcgt ccgcgccggg caacgagggc ttcgggaagg tccgttgcac gctcgccgaa
    11881 ggccacaacc cgctcgaggt ctcggccgtg gcgctggtca gcaacagggc ggcccccgcg
    11941 ccgcctaccc ccccgaaccc gacgctggac acttccggcg acgcgcaccg cgtgttcccc
    12001 tacgcccagc agcctcggtc cgtcgaggtc tcgggcggca ccggcggcat cgtcctgcag
    12061 agctgccaca cgctgacggc cacggtcgag gaccagaaca gcaggccgat cccggtggcg
    12121 aacgtcgacg tgcacgccga cggtcccgag gaccagctgt acttcggcac gcgcacggac
    12181 ggggccgagc ccggccggac cgacggcttc caggcccccg acgtggctca cgtgtcgaag
    12241 gagaacgcca agaactgcac gaacaacaac gacctgggcc agcagggtga ccacaacagc
    12301 ccgggacgcg acgacgccaa gcacatcgag tcgaccgacg ggacgtccaa cacgggcacg
    12361 ttccggttcg cgctgcgcag tgacttcgcc ggcgggacgt tcgtccaggt gtgggcagac
    12421 gtcgacgacg acgactcgcc ggacctgaac gaggcgagcg gcgggaccca gctcggctgg
    12481 gggggcccgg cccccgaccc ggtccaagag atattcgtga ccccgacgag cgccaacgga
    12541 tcgaacggct cgtgcgtcga gctcgaggtc ctcgctcgcc gcggcggcac gccgttcaac
    12601 gcggcgaacg tcgacatcca cgtccggggg ccggaaccgg ccccgacctt ctgtccggtc
    12661 gcgggcggcg ggacgagccg tccgcccgag agcggcggcc acagcaacag cacgcacgag
    12721 gacgggaccc tgcacgccga gggcgagacc gacgcgtccg ggaggttcgt gttcggagtg
    12781 tcgtccgcag cgtcgggcac cacccaggtg caggtgtgga tcgaccgcga cgacgacgac
    12841 acgctgaacg ccgagcccaa caagaccgcg acgatcacgt ggcagcccgc gggcgaccgg
    12901 tcgatctcgc tgacgagcag caagtcgcgc gtcaaccggg gtcgccgcgt gcggctgtcg
    12961 ggccggatcg agggcgacgt cgcgtgctcg gccgggcaga gcgtggacat ccaggcgaag
    13021 cgggtgcggg gcggggcctt cggcaccgtc aagaccgtca cgacgcaggc cgacggctcc
    13081 tacgcgaccc gggtcaagat gttcaaggcc cggaggttca gggccgtcgc gcaggccggc
    13141 gacccgtgct cgttcgcacg ctcgaacacc gtcaccgtcc gcgtcaaccg ttagcgcacg
    13201 gcggtcccgg ggtagggacc gtcgcatgat cgagtacggc tacaagctct cctccgagga
    13261 gcagtcgccg aacgacctcg tccgctacgc gcagcgcgcc gaggacgcgg ggttcacctt
    13321 cgccgcgatc tcggaccact tccacccgtg gacgagcaag cagggcgaga gcccgttcgt
    13381 gtggaacgtg atcggcgcga tcgcccacgc gacgcgcgag ctgaagctcg gcaccgcggt
    13441 gacgtgtccg acggtcaggg tccacccggc gatcgtggct cacgccgcgg cgacgagcgc
    13501 ggcgatgatg cccgggcggt tctggctcgg cgtcgggtcg ggcgagaacc tcaacgagca
    13561 cgtgacgggc cagcgctggc cgcagacgcg gatcaggcag gacatgatgg tcgaggccat
    13621 cgaggtcatc aggacgctgt tcgagggcgg catgcgaagc caccacgggc gctacttcac
    13681 ggtcgagaac gcgcgtctgt acacgctcga ggaggccccg cccatctacg tcgccgcaag
    13741 cggtgatcgt gccgcgaaga tcgcgggcga gcacggagac gggctcatct gcgtcgcgcc
    13801 gtcggacgag accgtgaagc ggttcgagga cgccggcggc tcgggcaagc cgaagtacgc
    13861 cgaggtcaac gtgtgctggg cccccaccga ggagcaggcg aagcagacgg ccctcgagtg
    13921 gtggcccgtc gcggggctcg gcggcacgct catgcaggag ctggcgctgc cgtcacactt
    13981 cgaggccgcg gccgagccgc tggacgcgga caaggtctcc gagacgctgg cggtcggccc
    14041 cgaccccgag gtgcacgtcg agaacatccg gaagttcgcc gaggccgggt tcacccacct
    14101 gtggatccac cagataggcc ccgaccagga cggcttcttc gacttctacg agaagcaggt
    14161 gctgccgaaa gtccgctagc agagcttccc gtcacgctcg gccaggcgcc gggccacctc
    14221 cctgtaacgg cccaccggga tcaggtgcac gccgtcgaac ccgccggact ccctcagctc
    14281 ctcgatctgg tcgcacgcga tctcgatccc catctccggg tcgtcgtcga gcttgtcgac
    14341 gatccggcgc gggatgtgga cgtcgggcag ggccgcgttc acgcgctgcg ccatgcggct
    14401 gctcgacagc accagcacgc ccgcgtacat gcggccctcg tagtccagcg acctccgcca
    14461 cgcgagcagg ttcgacgtcg cgtaggtgat ctgcacgaag atgaagtccg cgcgcgacct
    14521 ccacagcagg tcgcgccccg agcggacgac ggtccctatc cggaagtccg ggtatccctg
    14581 gaacagcggt ccagtgccga acgagcggac ctcgtcgatc atccctcgca cggtcaggtc
    14641 ctcggtccgg cgcccttcct tcggcgagtc gccgtacacg aacaggaagt ggtcgacgcc
    14701 gtaggcggcc gcggtcagca ggtcgcggcg gaagccgagg acgttgcggt cgcgcgcgtt
    14761 catgcacgcg atcgacagcc cgcccatgta cgcgacctcg tgcgcgaccg agacgctcga
    14821 gaccgtcgcg cggcccaggt ggttgtccgg gatcaggaac gcgtcgacga cgggcttcag
    14881 cacgtggatc tggtcgcgca cgatctcgat gtcggggcgg cgcgggggct ggatctcgca
    14941 gaggatgctg aagcgcccgg agtcgaggaa agaagacggc ggcggtggag gaggtgggac
    15001 cccggagccc gtgctcgtca tggcctcgga tataccaggc cgcgggttac cgggtcagtg
    15061 cgtgaggtcc tggacgacct tcttgacctt gcggacggcc ggattggcgc tcgcggggcc
    15121 cggcgtgccg aggccgccgt catcgccacc gtcctc
//
LOCUS       DASQDO010000560         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_976259, whole genome shotgun sequence.
ACCESSION   DASQDO010000560 DASQDO010000000
VERSION     DASQDO010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976259"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VEV43_15360"
     CDS             <1..408
                     /locus_tag="VEV43_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24944.1"
                     /translation="AGRSEIEKLSVWQKLVLTTLRTDEDPQVIGALSPEAFLDYVFDY
                     DFDYFPTARLRASSIKMTSRKTAAVGIGSTVVWFQRVDGEWKVDCRGAFEAVSVITKR
                     YAKKEGLTIRTTIFGLAEDFTTKVVPGDVWSKP"
     gene            complement(410..1231)
                     /gene="folP"
                     /locus_tag="VEV43_15365"
     CDS             complement(410..1231)
                     /gene="folP"
                     /locus_tag="VEV43_15365"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008714271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYP24945.1"
                     /translation="MTCLVMGIVNRTPDSFYDGGRMGLDESVRHAVALVEEGADVLDL
                     GGVRAGPGPHVTEQEERGRLLPLVEALRAEVDVPLSVETHRAGVAEAAIAAGAGIVND
                     VTMLSDPGLAGVVARSGVQLVVMHHGGQIRGRPRNPRYEDVVASVGAALLAGADEAIA
                     AGVDPSRIVVDAGLDFGKNTYHSLELVRRTQELAGLGYPLLVAPSRKDVVGETLGLPP
                     DDRLEGTLALVALSVWGGASIVRVHDVKATVRTVRMVEAVMGTRAPAAPVRGLWE"
     gene            complement(1228..2133)
                     /locus_tag="VEV43_15370"
     CDS             complement(1228..2133)
                     /locus_tag="VEV43_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYP24946.1"
                     /translation="MEATGTQPLLSERAPWLPWALLAVGVAAASISAILVRYADGADP
                     LAMSFWRCFAGAVALLPFATGGLRRAGSSVVRAPVVAGVFLALHFATWMTSVNLTTVA
                     ASVLLVSTTPVFVAIAALMIYGERLPGIAWAGILLATGGAAVVGGGSLEGSSLDGNAL
                     AVAGAATAGGYVMAGQVARRTLGILEYAVVTYAVSAVLLLVVCLVAGVDLAGYPAGTW
                     LAIAGLVVGPQLLGHTVINLVLSDIDATTVSVTIMAEPVIATALAFWLFDEVPTWLLY
                     PGGVAILAGIYMVSSARRDPVVIVE"
     gene            2176..>2355
                     /locus_tag="VEV43_15375"
     CDS             2176..>2355
                     /locus_tag="VEV43_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYP24947.1"
                     /translation="MAPQIAVGAIVVRDGRLLMVQRANEPGAGLWSLPGGRVEHGEYL
                     AAALRREVKEETGLDV"
BASE COUNT          390 a          854 c          819 g          292 t
ORIGIN      
        1 gcaggccgct cggagataga gaagctgtcg gtctggcaga agctcgtgct gacgacgctg
       61 cgaaccgacg aggacccgca ggtcataggc gcgctgtcgc ccgaagcgtt cctcgactac
      121 gtcttcgact acgacttcga ctacttcccg accgcacgac tccgagcgag ctcgatcaag
      181 atgacctcgc ggaagacggc cgccgtcggg ataggcagca cggtggtctg gttccagcgc
      241 gtcgacggcg agtggaaggt cgactgccgt ggggcgttcg aagccgtgag cgtgatcacg
      301 aagcgctacg ccaagaagga gggcctcacg atccgtacga cgatcttcgg cctggcggag
      361 gacttcacga ccaaagtggt gccgggggac gtctggtcga agccgtaggt cattcccaca
      421 gcccccgcac cggggccgcc ggcgctctcg tccccatcac ggcctcgacc atgcgcaccg
      481 tccgcaccgt cgccttcacg tcgtgcacgc gcacgatcga tgccccgccc cacaccgaca
      541 gcgcgacgag cgcgagcgtg ccttcgaggc ggtcgtccgg cggcagcccc agcgtctccc
      601 ccaccacgtc cttgcgcgac ggcgccacga ggagcgggta accgaggccc gctagctcct
      661 gcgtcctgcg cacgagctcc agcgagtggt acgtgttctt cccgaagtcg agcccggcgt
      721 ccacgacgat ccggctcggg tctactccgg ccgcgatcgc ctcgtccgcc cccgcgagca
      781 gggcggcccc cacggacgcg accacgtctt cgtagcgggg gttgcgcggg cgccctcgga
      841 tctggccccc gtggtgcatg acgaccagct ggacgcccga ccgcgcgacg acgcccgcga
      901 ggccggggtc ggagagcatc gtcacgtcgt tcacgatccc ggcccccgcg gcgatcgccg
      961 cctccgccac tcccgcccga tgcgtctcga ccgacagcgg gacgtcgacc tcggcacgca
     1021 gcgcctcgac cagcggcagg agccggccgc gctcctcttg ttcggtgacg tgggggccgg
     1081 gccccgcacg gacgcctccc agatcgagga cgtccgcccc ctcctcgacg agcgcgacgg
     1141 cgtggcgaac ggactcgtcg agccccatcc ggcccccgtc gtagaacgag tcgggcgtgc
     1201 ggttgacgat gcccatcacg agacacgtca ttcgacgatg acgacggggt cccgccgggc
     1261 gctcgagacc atgtagatgc cggcgaggat cgcgacgccg cccggataga gcagccacgt
     1321 cgggacctcg tcgaacaacc agaacgcgag cgcggtcgcg atgacgggct cggccatgat
     1381 cgtcacagac accgtcgtcg cgtcgatgtc cgacagcacg aggttgatca cggtgtggcc
     1441 gaggagctgg gggcccacca cgagccccgc gatcgcgagc caggtcccgg ccgggtaccc
     1501 ggcgaggtcg acacccgcga cgaggcagac gacgagcagc agcactgcgg acaccgcgta
     1561 cgtgacgacc gcgtactcga ggatgcccag ggtccgccgg gcgacctggc cggccatcac
     1621 gtatccgccg gccgtcgccg cgccggcgac cgcgagggca ttgccgtcga ggctcgaccc
     1681 ttcgagtgac cctccgccga ccaccgccgc cccaccggtg gcgagcagga tgcccgccca
     1741 cgcgatcccg ggaaggcgct ctccgtagat catcagcgcc gcgatcgcca cgaacacggg
     1801 agtggtcgag acgagcagga cggatgccgc gaccgtcgtc aggttgaccg aggtcatcca
     1861 cgtggcgaag tgcagcgcga ggaagacccc ggcgacgacc ggggctcgca ctaccgagga
     1921 ccccgctcgc cggagacctc cggtggcgaa cgggagcagt gcgacggcgc cggcgaagca
     1981 gcgccagaac gacatcgcga gcgggtcggc tccgtccgcg tagcgaacga ggatcgcgga
     2041 gatcgaagcg gccgccacgc ccacggcgag cagcgcccac ggcaaccacg gcgcgcgctc
     2101 ggagagcagc ggctgggtcc cggtcgcctc catggcgccc taccctaggt ccgcctaccc
     2161 gggagggatg acgccatggc gccacagatc gcggtcggtg cgatcgtcgt gagagacggc
     2221 aggttgctga tggtccagcg cgcgaacgag ccgggcgcgg ggctgtggtc gctgccgggc
     2281 ggccgcgtcg agcacggcga gtacctcgcc gccgcgctgc gccgggaggt caaggaagag
     2341 acgggactcg acgtc
//
LOCUS       DASQDO010000561         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_853639, whole genome shotgun sequence.
ACCESSION   DASQDO010000561 DASQDO010000000
VERSION     DASQDO010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853639"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VEV43_15380"
     CDS             complement(<1..437)
                     /locus_tag="VEV43_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24948.1"
                     /translation="MIRTSYLRVYQPLSEFSESERAAWGGRSEEIEQDEPALVRKWLL
                     GRALPDAEPIGAATDGAFVREVDGTTYVCPWRTRLRMLAGLLAFRTSVPEEVADAFVP
                     EAEARRAAAELAALGEDAPEVRSHILHANWHVPLRWFVAFDET"
     gene            536..1627
                     /locus_tag="VEV43_15385"
     CDS             536..1627
                     /locus_tag="VEV43_15385"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYP24949.1"
                     /translation="MSVFEHINGDGYEQVVFCSDDQSGLRAIIAIHSTALGPALGGTR
                     FYPYPAEDLALVDVLRLAKGMTYKAAAAGLDLGGGKAVIIGDPRRIKTEELLRAYGRF
                     VETLGGRYITAEDVGTALEDMDVVRRESRWVTGCSNTYGGSGDPSPVTAYGVLQGIKA
                     CALDVFGEPDLKGRSVALQGVGKVGHALCGYLVEEGAKVTIGDIDVDNLGRAVNDYGV
                     QTVSLADIHKQEVDIFAPCALGGIVNDDTISEFRCKIIAGAANNQLARQEHGDKLRDL
                     GILYAPDFVINAGGLINVEDELRGYDQKRAMKRVEGIYKQLQLIFAMARERNLSTARA
                     AEEHAQERIRKISRIRLVRTAEFGPSSSN"
     gene            1632..>1891
                     /locus_tag="VEV43_15390"
     CDS             1632..>1891
                     /locus_tag="VEV43_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYP24950.1"
                     /translation="MTDPLRIIGDGRGMPDAPLPEGLAEKDLIELFRWLVLLRTFDER
                     AVALQRQGRIGTYALYWGEEGTQAGALYACEDGDWVYPSYRQ"
BASE COUNT          322 a          635 c          669 g          265 t
ORIGIN      
        1 tcggtctcgt cgaaggcgac gaaccacctg agcgggacgt gccagttggc atggaggatg
       61 tgcgaccgca cctcgggcgc gtcctcgccc agcgcggcga gctcggccgc agcccggcgc
      121 gcctcggcct cggggacgaa cgcgtcggcc acctcttccg ggacgctcgt cctgaacgcg
      181 agcagaccgg cgagcatgcg cagccgcgtg cgccagggac agacgtaggt cgtgccgtcg
      241 acctcgcgca cgaacgcgcc gtcggtcgcg gccccgatgg gctcggcgtc gggaagcgcg
      301 cgcccgagga gccacttgcg gacgagcgcc ggctcgtcct gctcgatctc ttcgctgcga
      361 ccgccccagg cggcccgctc ggactcggag aattcggaca gcggctgata cacgcgcagg
      421 taggaggtcc tgatcatcgt gcaaatgcta aacgcacgcc gagcggccgg gcggtaaagt
      481 ctcctccgca cctcggcgca tccggcgccc tccagaacgg ggagacctca agagcatgag
      541 cgtgttcgag cacatcaacg gcgatggcta cgagcaggtg gttttctgct ccgacgacca
      601 gtcggggcta cgcgcgatca tcgcgatcca ttcgactgca ctggggcccg cgctcggcgg
      661 gacgcgcttc tacccgtacc ccgcggagga cctggcgctc gtcgacgtgc tcaggctcgc
      721 gaaggggatg acgtacaagg cggccgccgc ggggctcgac ctcggcgggg gcaaggccgt
      781 catcatcggc gacccgcgcc gcatcaagac cgaggagctg ctgcgtgcgt atggacgctt
      841 cgtagagacg ctcggcgggc gctacatcac ggccgaggac gtcggcactg cgctcgagga
      901 catggacgtc gtacgtcgtg agagccgctg ggtgacggga tgctcgaaca cgtacggcgg
      961 gtcgggcgat ccttcaccgg tgacggcata cggcgtcctc caggggataa aggcgtgcgc
     1021 gctcgacgtg ttcggcgaac ccgacctcaa gggccggagc gtcgcgctgc agggcgtcgg
     1081 caaggtcggc cacgcgctgt gcggctatct cgtcgaggag ggcgcgaagg tcacgatcgg
     1141 cgacatcgac gtcgacaacc tcggccgcgc ggtgaacgac tacggcgtgc agacggtgag
     1201 cctcgccgac atccacaagc aggaggtcga catcttcgcc ccctgcgcgc tcggcggcat
     1261 cgtcaacgac gacacgatct cggagttccg ctgcaagatc atcgcggggg ccgccaacaa
     1321 tcagctcgct cgccaggagc acggcgacaa gctccgcgac ctcggcatcc tctacgcccc
     1381 cgacttcgtc atcaacgcgg gtggcctcat caacgtcgag gacgagctgc gcgggtacga
     1441 ccagaagcgt gcgatgaagc gcgtggaggg catctacaag cagctccagc tgatcttcgc
     1501 gatggcgcgc gagcgcaatc tctcgacggc ccgtgccgcg gaggagcacg cgcaggagcg
     1561 catccgcaag atcagccgca tccggctcgt gcgcaccgcg gagttcgggc cgagctcgag
     1621 caactgacgc cgtgaccgac cctctgcgga tcatcggcga cggccgcggg atgccggacg
     1681 cgccccttcc cgaaggactc gccgagaagg acctgatcga gctgttccgg tggctcgtgc
     1741 tgctgcgcac gttcgacgag cgcgcggtcg cgctgcagag gcagggacgc atcgggacgt
     1801 acgcgctgta ctggggcgag gaggggacgc aggccggagc gctgtacgcg tgcgaggacg
     1861 gcgactgggt ctatccgtcc taccggcaga a
//
LOCUS       DASQDO010000562         7306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1159289, whole genome shotgun sequence.
ACCESSION   DASQDO010000562 DASQDO010000000
VERSION     DASQDO010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159289"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VEV43_15395"
     CDS             <1..574
                     /locus_tag="VEV43_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24951.1"
                     /translation="MRRMVLLLAVATGLGAAAVPAAARPSFEHLAYDPVGDHNGMEHI
                     DLTALYARQRSGALQVRFEFLDLDDELRHVSGVAWDLGYGGTRYCCTISARTRAAAQV
                     YDFTYWNRCDGCGRTTIELDGSFDAATETITVSIPLDLTPLRPGDVLSGCGYPAEWRY
                     RRCGAHYSGCTSPGMYGYDCFEVRKTFTIR"
     gene            610..1776
                     /locus_tag="VEV43_15400"
     CDS             610..1776
                     /locus_tag="VEV43_15400"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYP24952.1"
                     /translation="MATKPRDPELAHWAHLIEQLRWAAFLLDRDFRLVWASPDIERFL
                     GSPTDDEIGYGKHVLDALTSDAWRRAATPGSRSRLAEELATVVIGHARSQGLDLGFVP
                     ERFAALRETEPRPFTRAFASEFEYVEPGGDSDLPVMRVNVLAMPLRDESGELTGALVL
                     GYMSVRPGLVSLLARGDEAMYEGMAKLVEPSSREGAILFCDLEGSTDLARTLPSAQYF
                     RLIRSLWTEIDGLVASNRGIVGKHAGDGATAFFLVDDLGSASEAAAAAIRTARGIHER
                     SAGVFGDALDASCLMRVGLHWGSSLYIGQLVPGGRLDVTALGDAVNECARIQESAEPQ
                     QTLVSKELIERLSDDDAAAAGVDPEKIRYRPLAQLPAAGDKAVATAGAISVTPL"
     gene            complement(1783..2517)
                     /locus_tag="VEV43_15405"
     CDS             complement(1783..2517)
                     /locus_tag="VEV43_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HYP24953.1"
                     /translation="MPDIAEVYTAARKEFADFVAGLSDDELARDVPATPGWSVHDVVA
                     HISGVVRCVAAGDFPAEFFADIGAQPGIVVLNEWTAGQVEAGRPRPLNELLDEWADAT
                     EAIMPMLRGDEPWPGDVVPFAAYILTCDLGVHQHDVYGALGIVRDRDSTPVKIGFSVY
                     TGGLGVRARTYGKPALRIVTETKDVTAGDGEPAATVRGSRFELFRALSGRRSLDQLRS
                     YDWEGDPEPFLELFYPYGIREDALVE"
     gene            2644..3300
                     /locus_tag="VEV43_15410"
     CDS             2644..3300
                     /locus_tag="VEV43_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24954.1"
                     /translation="MASYTKWCAALLAIFVLVAIPACGGDEGESGGASGGEPAAGAAP
                     GEDMSGDTEDEGDSGSAEGTSGEGGDTGEGTQETGTGGDPNARFNLIASPDNVECSYV
                     PGGDLSGADLLNVHFYFLIIGGNPPDVPPVSITGQSDTGLTTSYNGGPHNQAVNTAQF
                     ALREGDFGRSHSVTLTVDAGNEVSETSESDNRIRVSVALPSPRPTQTIDPLSCSATSG
                     "
     gene            3324..5147
                     /gene="ggt"
                     /locus_tag="VEV43_15415"
     CDS             3324..5147
                     /gene="ggt"
                     /locus_tag="VEV43_15415"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYP24955.1"
                     /translation="MRGNAPSGALLVIAVVAASTFPLHAASATEGSNFREAVSSNGGV
                     VASVSAQAGEVGVRILDRGGNAVDAAVATTLAVGVTRPDLCGIGGGGFLAYRGADGEV
                     ATLDFREEAPRAITSDAYQGTGIYRSFTGHKTVGAPGTVAGMAKVAKRFGTMPLAALV
                     RPARRLAARGVVVTEDLSASMGANSERLRLFPASARIYLVEGTAPYPPGSTLEQKGYA
                     RSLRLIEERGPRAFYRGRIARLIVRDMKENAGAYPGDDGLMRRGDLRRYRAKWRRPLQ
                     TTYRGHRVLAVGPPTSGGLLAIEMFNILKNFDVPSFEPFGADRLHYTAEAQKIAWADR
                     DAYVADPDHADVPTSLLASEAYAAQRAEEIDRDQAKDYEPAGRPGKPTPAPGYDQPGA
                     HHTTHVSVVDKDGNAVSVTCTVEFLFGSAVVAPGAGFILNNQLTDFSAPGTANEPEPG
                     KRPRSSTTQLIVSKRGRPVAVLGGAGGPRIPMGVIMTGQNVMDFGLDLAHAIDAERLN
                     EPTCCQMTLEDLRVGPETQLELERRGHRIVREGEYGGTPIVQAAGVNLETGKQEAVSD
                     PRGEWGSAGQEKSRGAAAVALGALSLLAGLGLRRTRRAAET"
     gene            5222..6535
                     /locus_tag="VEV43_15420"
     CDS             5222..6535
                     /locus_tag="VEV43_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24956.1"
                     /translation="MRIARLVALGAALSLLSAVPVAAGHDESLHSDNIEEIARSPIRV
                     PGGEIADGSDLAFEGNLIVAGSYSGTALFKILKRAPYVKQVGFHNCTGSQGDVNVYRG
                     LVFVSLDSGQSSGSVGCPESEGMEGLRIVDVSNPKKPEQVKFVETDCGSHTNVINPHG
                     DKVYVYVMSYPLGVPSATCSPVSHRKVSIVEVDVDAPQKAKVVGTLDVSPEIGCHDVT
                     IMPDGKHAAAACIGESQIWDVSDPASPVIVSRIVNPAIEIHHGTGVTWDQKYLAIADE
                     FAGSVTGECAGDRSSPVGALWFYDISDLSLPQLVGYYNVPRGGRPDTAEEASYIACTS
                     HNFNILPMKDPKRYVAAVGYRASGLSVVDFSDPAAAKEIAHYQQLEEGNIPDVWSAYW
                     YNGRIYANDNGASRGLSVYEMKGTSAKEVKFFKTRMNPQVQLADF"
     gene            complement(6532..>7306)
                     /locus_tag="VEV43_15425"
     CDS             complement(6532..>7306)
                     /locus_tag="VEV43_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24957.1"
                     /translation="DKTQPVEIATLPEGGGHTQTCVLGCRYAYGSSGVIVDLRDPINP
                     QIAGSWTKPESGVYAHDVTEVSPGMVLTAGKPMLLLDARRNPTSPKVVAAGYDDAYPI
                     GLHSTDWPNRGEDPFILGSNEALASARCDLSDGSFSVWSSRGFERTRTFTLVDSWALA
                     NGTYADGSPAAGGMGCTAHWFDEHPTFQSGGVVALGAYEHGTRFLYVDEAGTISERAG
                     SSHTPGRRRPRTGSTTGSSTRSTTHAGSTCSATPGSCAD"
BASE COUNT         1174 a         2522 c         2538 g         1072 t
ORIGIN      
        1 tatgagacgg atggtgttgc tgctggcggt cgcgacgggc ctcggcgcgg ccgcggtccc
       61 cgccgcggcg cgaccttcgt tcgagcacct cgcttacgac cccgtggggg accacaacgg
      121 gatggagcac atcgacctga ccgcgctcta cgcgaggcag cgctcgggcg ctctccaggt
      181 gcggttcgag ttcctggacc tggacgacga gctgaggcat gtctcgggcg tcgcctggga
      241 cctcggctac ggggggacgc gctactgctg cacgatctcg gctcggacga gagctgccgc
      301 ccaggtctac gacttcacgt actggaaccg ctgcgacggc tgcggccgga cgacgataga
      361 gctcgacggc tccttcgacg cggcgaccga gacgatcacg gtctcgatcc cgctcgacct
      421 caccccgctg cggccgggcg acgtcctgtc gggctgcggc taccccgcgg agtggcggta
      481 ccggaggtgc ggtgctcact acagcggctg cacctcaccg ggcatgtacg gctacgactg
      541 cttcgaggtc cgcaagacgt tcacgattcg ctgagggccg cacgtactac gggtcgtatc
      601 ccttagccca tggcgaccaa gccccgggac cccgagctcg cccactgggc ccacttgatc
      661 gagcagctcc gctgggccgc gttcctcctc gaccgcgact tccgcctcgt gtgggcatcc
      721 cccgacatcg agcgcttcct cggctcccct acggacgacg agatcggtta cggcaagcac
      781 gtcctcgacg cgttgacgag cgacgcctgg cgacgggcag cgacgccggg gagccggtcg
      841 cgcctcgcgg aagagctcgc gaccgtcgtg atcggccacg ctcgctcgca agggctcgac
      901 ctcggcttcg tccccgaacg gttcgccgcc ctgcgagaga cagagccgcg gcccttcacc
      961 cgagccttcg cgtcggagtt cgagtacgtc gagcccggag gcgacagcga cctcccggtc
     1021 atgcgcgtga acgtgctcgc catgccgttg cgcgacgaga gcggcgagct gaccggggcc
     1081 ctcgtcctcg gctacatgag cgtgcggccc ggcctcgtct ccttgctcgc acgcggcgac
     1141 gaggcgatgt acgaggggat ggcgaagctc gtcgagccca gctcccgcga gggcgccatc
     1201 ctgttctgcg acctcgaggg gtcgaccgac ctcgcacgga cgttgccgag cgcgcagtac
     1261 ttccgtctga tcaggagcct gtggacggag atcgacgggc tcgtcgcgag caaccggggg
     1321 atcgtgggca agcacgcggg ggacggcgcg acggcgttct tcctcgtgga cgacctcggc
     1381 tccgcgtcgg aggcggcggc ggctgcgatc cggaccgccc gcggcatcca cgaacgttcg
     1441 gccggcgtgt tcggggacgc tctcgacgcg tcgtgtctta tgcgcgtcgg gctgcactgg
     1501 ggaagctccc tctacatcgg gcagctcgtc cccggcggac gtctcgacgt caccgccctc
     1561 ggcgacgcgg tcaacgaatg cgcgcgcata caggagtccg cggagccgca acagaccctc
     1621 gtctcgaaag agctgatcga acgtctgtcg gacgacgacg cggccgctgc cggcgtcgac
     1681 cccgagaaga tccgctaccg cccgctggcg cagctgccgg ccgcggggga caaggccgta
     1741 gcgacggcag gagcgatctc ggtgacgccg ctgtaggtcg ccctactcga cgagcgcgtc
     1801 ctcccgtatc ccgtacggat agaagagctc gaggaacggt tcgggatcgc cctcccagtc
     1861 gtacgagcgg agctggtcga ggctccgcct gccggagagc gcacggaaga gctcgaagcg
     1921 ggagccgcgc acggtcgcgg cgggctcgcc gtcgccggca gtgacgtcct tcgtctcggt
     1981 gacgatgcga agcgcgggct tgccgtacgt cctcgccctg acgccgaggc cgcccgtgta
     2041 caccgagaac ccgatcttca ccggagtcga gtcgcggtcg cgcacgatgc cgagcgcgcc
     2101 gtacacgtcg tgctgatgga cgccgaggtc gcaggtcagg atgtaggcgg cgaacggcac
     2161 gacgtcaccg ggccagggct cgtcgccgcg cagcatgggc atgatggcct cggtcgcgtc
     2221 ggcccactcg tcgagcaact cgttcaaggg gcgcggccgc cccgcctcga cctggccggc
     2281 ggtccactcg ttgaggacga cgatcccggg ctgcgcaccg atgtcggcga agaactccgc
     2341 ggggaagtca cccgccgcga cgcaccggac gacgccggag atgtgagcca cgacgtcgtg
     2401 gaccgaccac cccggtgtag ccgggacgtc ccgcgccagc tcgtcgtcgg agagcccggc
     2461 cacgaaatcc gcgaactcct tgcgagcagc cgtatagacc tcggctatgt ccggcatgat
     2521 tttcgaaccc tagcgtggga ggaccgcgcg ccaggtgtca ggtagccgac ttctccggta
     2581 agcaatccgg gtcatcatct cgacaggctg gacctgcagg tgagacgaga caaggagacg
     2641 tccatggcct cctacaccaa gtggtgcgcg gctttgctcg cgatcttcgt gctggtggcg
     2701 atccccgcat gcggcggtga cgaaggcgaa tcggggggag cttccggcgg tgagcccgca
     2761 gccggcgcag cgcccggcga agacatgagc ggcgacaccg aggacgaagg cgactccggt
     2821 agtgccgagg gaacgtccgg cgagggaggc gacaccggcg aggggaccca ggagaccggt
     2881 acgggcgggg acccgaacgc tcgcttcaat ctcatcgcgt cgccggacaa cgtcgagtgc
     2941 tcctacgtcc ccggcggaga cctgagcggc gccgacctcc tgaacgtgca cttctacttc
     3001 ctgataatcg gcggcaatcc acccgacgtc ccgccggtgt cgatcaccgg ccagagcgac
     3061 accgggctga cgacctccta caacggaggc ccccacaacc aggccgtcaa caccgcgcag
     3121 ttcgcgctga gagagggtga cttcggccgg agtcactccg tgacgctgac ggtcgacgcg
     3181 ggcaacgagg tctcggagac gtcggagtcg gacaaccgca tcagggtgag cgtcgcgttg
     3241 ccgtcgcccc gccccacgca gacgatcgac ccgctgagct gctcggctac ttccggctag
     3301 gccagccgaa ctaggatcga ctcatgcggg ggaacgcgcc gagcggcgcg ctgttggtga
     3361 tcgccgtggt cgcggcctcc acgttcccgt tgcacgccgc gtccgcgacc gagggctcga
     3421 acttccgcga ggccgtgagc agcaacggcg gggtcgtcgc gagcgtctcc gcgcaagcgg
     3481 gtgaggtcgg cgtccggatc ctcgaccgcg gcgggaacgc agtcgatgcc gcagtcgcca
     3541 cgacccttgc cgtaggcgtg acgcggccgg acctgtgcgg gatcggtggc ggtggcttcc
     3601 tcgcctaccg gggcgcggac ggagaggtcg cgaccctgga cttccgcgaa gaggcaccgc
     3661 gcgccatcac gtccgacgcc tatcagggca cgggcatcta ccgctctttc acgggacaca
     3721 agaccgtggg cgcgccgggc accgtcgcgg ggatggccaa ggtggcgaaa cgtttcggca
     3781 cgatgcctct cgccgcgctg gtccgtcctg cgaggaggct cgcggcgcgc ggcgtcgtcg
     3841 tgacggaaga cctctcggct tcgatgggag ccaactccga gcggctgcgg ctcttccccg
     3901 catcggctcg catctacctg gtcgagggca cggctccgta tccgccgggc tcgacgctcg
     3961 agcagaaggg ctacgcgcgc tcgttgcgac tgatagagga gagggggccg cgcgcgttct
     4021 atcgcggccg gatcgcccgg ctgatcgtgc gcgacatgaa ggagaacgcc ggcgcttatc
     4081 cgggcgacga cgggctgatg cgccgcggcg acctgcggcg gtatcgggcg aagtggcgga
     4141 ggccgctcca gacgacgtac cgcggacacc gcgtcctcgc cgtcggcccg ccgacgtcgg
     4201 gcggcctgct ggcgatcgag atgttcaaca tcctgaagaa cttcgacgtc ccgtcgttcg
     4261 agccgttcgg ggccgatcgt cttcactaca cggccgaggc ccagaagatc gcgtgggcgg
     4321 atcgcgacgc ctacgtcgcg gaccccgacc acgccgacgt cccgacgagc ttgctggcga
     4381 gcgaggccta cgccgctcag cgggccgagg agatagaccg cgaccaggcc aaggactacg
     4441 agcccgccgg ccgacccggc aagccgactc ccgcaccggg ctacgaccag ccgggcgcgc
     4501 accacacgac gcacgtctcg gtcgtcgaca aggacggcaa cgcggtctcg gtgacgtgca
     4561 ccgtcgagtt cctgttcggc agtgccgtcg tcgcgccggg cgcgggcttc atcctcaaca
     4621 accagctgac ggacttctcg gcccccggga ccgcgaacga gcccgagccc ggcaaacgcc
     4681 cccggtcgtc gacgacgcag ctgatcgtct cgaagcgcgg gcggccggtt gcggtcctcg
     4741 gcggtgcggg cgggccccgg atcccgatgg gcgtgatcat gacgggacag aacgtcatgg
     4801 acttcggtct cgacctcgcg catgcgatcg acgccgagcg tctcaacgag ccgacgtgct
     4861 gccagatgac gctcgaggac ctccgggtgg ggccggagac gcagctcgag ctcgaacggc
     4921 gcggtcatcg gatagtgcgc gaaggggaat acggcgggac tccgatcgtc caagccgccg
     4981 gagtgaacct cgagaccggc aagcaagagg cggtctcgga cccgcgcggc gaatggggga
     5041 gcgcgggcca ggagaagagc cggggtgccg ccgccgtcgc gctgggcgcc ctctcgctcc
     5101 tcgcggggct cgggctgcgc cggacccgac gcgccgccga gacgtagtcc tcgccggcgt
     5161 cgcgaggatt cgcccgattt gcggcgaatg actcccccgg acgcgaggag gaggatctcg
     5221 gatgcgaatc gctcgactgg tcgcgctcgg cgcggccctg tctttgctct cggccgttcc
     5281 cgtcgccgcc ggccacgacg agagcctgca ctccgacaac atcgaggaga tcgcgcgcag
     5341 cccgataagg gtccccggcg gcgagatcgc cgacgggtcc gacctcgcgt tcgaagggaa
     5401 cctgatcgtc gccgggtcct acagcggcac ggccctgttc aagatcttga agcgcgctcc
     5461 ctacgtgaag caggtcggct tccacaactg cacgggctcg cagggtgacg tcaacgtcta
     5521 ccggggcctc gtgttcgtgt cgctcgactc cggccagtcc tccggaagcg tcggctgtcc
     5581 cgagtcggaa gggatggagg gccttcggat cgtcgacgtc tccaacccga agaagccgga
     5641 gcaggtgaag ttcgtcgaga ccgactgcgg gtcgcacacg aacgtcatca acccgcacgg
     5701 cgacaaggtc tacgtgtacg tgatgtcgta cccgctcggc gtgccgagcg cgacctgcag
     5761 cccggtctcg caccgcaagg tctcgatcgt cgaggtcgac gtcgacgcgc cgcagaaggc
     5821 gaaggtcgtg gggaccctcg acgtctcgcc cgagatcggg tgccacgacg tgacgatcat
     5881 gcccgacgga aagcacgcgg cagccgcgtg catcggcgag tcgcagatct gggacgtaag
     5941 cgacccggcg agcccggtca tcgtctcgag gatcgtcaac cccgcgatcg agatccacca
     6001 cgggacgggc gtgacctggg accagaagta cctcgcgatc gccgacgagt tcgccggctc
     6061 ggtgaccggc gagtgtgcgg gggaccgcag cagcccggtc ggcgcgctgt ggttctacga
     6121 catctcggat ctctcgctgc ctcagctcgt gggctactac aacgtcccgc gcggcggacg
     6181 gcccgacacc gccgaagagg cgtcgtacat cgcatgcacc tcgcacaact tcaacatcct
     6241 gccgatgaag gaccccaaga ggtacgtcgc cgcggtcggg taccgcgcct cgggcctgtc
     6301 ggtcgtcgac ttctccgacc ccgccgccgc aaaggagatc gcccactacc agcagctcga
     6361 ggagggcaac atccccgacg tgtggtcggc ctactggtac aacggccgga tctacgcgaa
     6421 cgacaacggc gcgagccggg ggctgagcgt ctacgagatg aagggcacgt ctgccaagga
     6481 agtgaagttc ttcaagacgc gcatgaaccc ccaggttcag ctcgccgact tctagtcagc
     6541 gcagcttccc ggtgtagcgg agcacgtcga tcccgcgtgc gtagtcgacc gcgtagacga
     6601 tccggtcgtc gagccagtac gcggccgacg tctccccggc gtgtgggagg aaccagccct
     6661 ctcggagatc gtccccgcct cgtccacgta caggaaccgg gtgccgtgct cgtaggcgcc
     6721 gagcgcgacc acgccccccg actggaacgt cggatgctcg tcgaaccagt gggcggtgca
     6781 gcccatgccc ccggcagccg gagagccgtc ggcgtaggtc ccgttcgcga gcgcccagct
     6841 gtccacgagc gtgaacgtcc gggttcgttc gaagcccctg gagctccaca cggagaagga
     6901 gccgtcgctc aggtcgcagc gcgccgaagc gagcgcctcg ttcgatccga ggatgaacgg
     6961 atcctcgccg cggttcggcc aatcggtcga gtgcagcccg atcgggtagg cgtcgtcgta
     7021 gccggccgcg acgaccttcg gcgaggtcgg gttgcgccgc gcgtcgagca gcagcatcgg
     7081 cttccccgcg gtcaggacca tccccggcga cacctccgtt acgtcgtgcg cgtagacacc
     7141 ggactcgggc ttggtccaac tgccggcgat ctgcggattg atggggtcgc ggaggtcgac
     7201 gatcacgccg ctggatccgt acgcgtaccg gcagccgagc acgcaggtct gcgtgtgtcc
     7261 gccgccttcg ggcagcgtcg cgatctcgac cggttgggtc ttgtcc
//
LOCUS       DASQDO010000563         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_364236, whole genome shotgun sequence.
ACCESSION   DASQDO010000563 DASQDO010000000
VERSION     DASQDO010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364236"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VEV43_15430"
     CDS             complement(<1..204)
                     /locus_tag="VEV43_15430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYP24958.1"
                     /translation="MRHRIPADVLLLATVLFWSFNFTVVKYAVTHGWEPLSYSSVRFA
                     IGAVLFSAVTYGREGSLRVQRRDV"
     gene            261..533
                     /locus_tag="VEV43_15435"
     CDS             261..533
                     /locus_tag="VEV43_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24959.1"
                     /translation="MAEPTIDDIDYLVGPATPHFAPHLRARVRGLIRDLPAEHPVRRY
                     GEEKIELLERLQLSSSKAEEGPLEPRDRLGWERLPSHGAASAGEEH"
     gene            530..1270
                     /locus_tag="VEV43_15440"
     CDS             530..1270
                     /locus_tag="VEV43_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYP24960.1"
                     /translation="MSFEGKSVLVTGGTRCIGKAIALRFAEEGAARVAVSYFRNDQAA
                     EETAEELRGLGTEPVLLRGNLGDPAKAREVVDGAGAIDVLVSNAATGVILPVLEATEK
                     HWDWTLNANARALLTLARHAVPTMTAGSSIIAISSMGGERVLDNYSLVGTSKAALESL
                     VRYLAVELAPRGIRVNAVSAGLVETGALEFFPDRAEMVERYRDRTPAGRLVEPRDVAD
                     VVAFLASPAAEMIRGQAVVVDGGYSVVA"
     gene            1267..1875
                     /locus_tag="VEV43_15445"
     CDS             1267..1875
                     /locus_tag="VEV43_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24961.1"
                     /translation="MSTVDRFAHDAFFIDQLIRPVANLYRVSTLGGDGKSPGDPLAFV
                     RQKKLAIKEDIRFFADQDEGEELFRLKARGLMEFRGRYDVLLPEGGRAGVLEKVFGAS
                     LLRSTWRLLDAEEQEVGMAFEKSAPVALLRRAIDLVPYGELIPIVFHFTITAGGQPVG
                     GLRRPIGLRDRYILDLSGDADWRIDRRTAVALGIALDALQSR"
     gene            complement(2043..>2404)
                     /locus_tag="VEV43_15450"
     CDS             complement(2043..>2404)
                     /locus_tag="VEV43_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HYP24962.1"
                     /translation="FQEIRDNFVRVLEALIEGGSKVAIATHDDWLVERSLELVERHGL
                     GPDDYEFQMLLGVREALGDRLVRDGHRLRIYVPYGRRWYEYSMRRLQENPKIATYVAR
                     DTIGRLVPGGNGGGTTG"
BASE COUNT          357 a          860 c          851 g          336 t
ORIGIN      
        1 gacgtcgcgc cgttgcaccc gcagcgagcc ctcgcgtccg taggtcaccg ccgagaagag
       61 gacggcaccg atcgcgaacc ggacggacga gtacgagagc ggctcccacc cgtgcgtgac
      121 cgcgtacttg acgacggtga agttgaagct ccagaagagg acggtcgcga ggaggaggac
      181 gtcggccggg atccggtgcc gcattgccgc ccgaccatag ccaggccccg accgcggccc
      241 ggcggtagag tcggcgtccg atggccgagc ccacgatcga cgacatcgac tacctcgtcg
      301 ggcccgccac cccgcacttc gcgccgcatc tacgcgctcg cgtgcgcggc ctgatccgcg
      361 accttcccgc cgagcacccg gtccgccgct acggggagga gaagatcgag ctcctggagc
      421 gcctgcagct ctcgtcgtcg aaggcggagg agggaccgct cgagccgcgc gaccgcctcg
      481 gctgggagcg gctgccgagc cacggcgccg cctcggccgg cgaagagcat tgagcttcga
      541 gggcaagtcc gtcctcgtca cgggtgggac gcgctgcatc ggcaaggcga tcgcgctccg
      601 gttcgcggag gagggcgcgg cgcgggtagc cgtcagctac ttcaggaacg accaggctgc
      661 tgaagagacg gccgaggagc tccgcgggct cggcacagag ccggtcctcc tgcgcggcaa
      721 cctcggggac ccggcgaagg cgcgggaggt cgtcgacggt gccggcgcga tcgacgtgct
      781 cgtgtcgaac gcggccaccg gggtcatcct ccccgtcctc gaggcgaccg agaagcactg
      841 ggactggacg ctgaacgcga acgcacgcgc cctcctgacc ctcgctcggc acgcagtccc
      901 cacgatgacg gcggggagct cgatcatcgc catctcgagc atgggcggtg agcgcgtgct
      961 cgacaactac tccctcgtcg ggacgtcgaa ggcggcgctc gagtcgctcg tccgctacct
     1021 cgccgtcgag ctcgcgccgc ggggcatccg cgtcaacgcc gtctcggcgg gcctcgtcga
     1081 gaccggtgcg ctcgagttct tcccagaccg ggcggagatg gtcgagcgct accgcgaccg
     1141 cacgcccgcc gggcgcctcg tcgagccccg agacgtggcc gacgtcgtcg cgttcctcgc
     1201 gtcgcccgcg gcggagatga tccgcggcca ggcagtggtc gtcgatggtg gatactcggt
     1261 ggtggcatga gcaccgtcga ccgcttcgcg cacgacgcgt tcttcatcga ccagctcatc
     1321 cggccggtcg ccaacctcta ccgcgtctcg acgctcggcg gggacgggaa gagccccggc
     1381 gacccgctcg cgttcgtgcg ccagaagaag ctcgcgatca aggaggacat ccggttcttc
     1441 gccgatcagg acgaaggcga ggagctcttt cgcctgaagg cgcgcggcct gatggagttc
     1501 cggggccgct acgacgtcct cctccccgaa ggcggccgtg cgggcgtgct cgagaaggtc
     1561 ttcggggcgt cccttctccg gtcgacgtgg cgactcctcg acgcggagga gcaagaggtc
     1621 gggatggcgt tcgagaagag cgcgcccgta gcgctcctgc gacgcgcgat cgacctcgtc
     1681 ccctacggcg agctgatccc gatcgtcttc cacttcacga tcaccgccgg cggccagccc
     1741 gtcggcggcc tccgccgccc gatcggcctg cgcgaccgct acatcctcga cctgagcggc
     1801 gacgccgact ggcggatcga ccgccgcacg gccgtcgccc tggggatcgc gctggacgcg
     1861 ctccagtcgc gctgagccgg ccgcgggctt tcgtcccacc ccggcacgaa gtcgtcacgg
     1921 agcgtcaccc accggcacgc cgggccgcac cacgctggac gcgaggggtg ttcaggtggg
     1981 ggtcccgggg gaagtctcgc gaatgcgtcc gcggacctga tgcgccggtc gacgcgggcc
     2041 ggtcagccgg tcgtgccccc gccgttgccg ccggggacga ggcggccgat cgtgtcccgc
     2101 gccacgtacg tggcgatctt ggggttctcc tggagccgac gcatcgagta ctcgtaccag
     2161 cgacgcccgt acggcacgta gatccggagg cggtggccgt cgcggacgag acggtcgccg
     2221 agggcctcgc gcacccccag gagcatctgg aactcgtagt cgtcagggcc cagcccgtgc
     2281 cgctcgacga gctcgagtga ccgctcgacg agccagtcgt cgtgcgtggc gatcgcaacc
     2341 ttggagccgc cctcgatcag cgcctcgagg acccggacga agttgtcgcg aatctcctgg
     2401 aagt
//
LOCUS       DASQDO010000564         6675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_364309, whole genome shotgun sequence.
ACCESSION   DASQDO010000564 DASQDO010000000
VERSION     DASQDO010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364309"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VEV43_15455"
     CDS             complement(<1..456)
                     /locus_tag="VEV43_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24963.1"
                     /translation="MRRAAALFAGIALALVWSQPASALCAEPPSLGRAIAETSVVFAG
                     TVTDTSSEGRWATVAVSDVWKGDVAAEVEVRGGPEDPPGPISASSSVERTYRRGETYL
                     VLPYAGSGEVFSDNACSGTTVYREGLARLRPAGAHAPPEEPEAASSGRTP"
     gene            530..1516
                     /locus_tag="VEV43_15460"
     CDS             530..1516
                     /locus_tag="VEV43_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009192596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYP24964.1"
                     /translation="MQGRIVVTGGLGFIGSAVVRRLLGKGLEVTNLDAGTYAADPRRL
                     PPDVDTKRVDVAADPLDEHLAGARVVVHLAAETHVTRSETDESAFFRTNVDGTRTVLA
                     AAERAGAELLVHFSTDEVYGPALEAPFVEADKEPGEGLATSAYARSKALADDLALGYE
                     GDVRVLVVRPTNCFGPWQHPEKAIARWTVRALSGQDLPVWGDGGYVRDWMFVDDVCDA
                     VEVLVDERPAPGAYNVGPEGDPVSNVEIARDIARLAGRGDDAVYLTAYDRPSHDRRYS
                     VDSSKMRALGWRPRVGVRDGLRATVDWYRAHRDWWEPLVAEAESLYDDRVPR"
     gene            1513..1980
                     /locus_tag="VEV43_15465"
     CDS             1513..1980
                     /locus_tag="VEV43_15465"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HYP24965.1"
                     /translation="MTSIDGVVVTPVERHEDGRGFFSEIARFGEYRQANHSHSQRGVL
                     RGLHYHLSQADLWYVVRGTAQVALADLRGDEPVVETITVGESAPCTIYVPPRVAHGFL
                     ALTDVDLVYLVTQEYDASDEHGVAWDDPTLAIPWALEDPILSDRDRSNPQLDG"
     gene            1973..2803
                     /locus_tag="VEV43_15470"
     CDS             1973..2803
                     /locus_tag="VEV43_15470"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HYP24966.1"
                     /translation="MVSGRVLVTGGDGQVGAALRALLPHGDFRSHDELDVTDAAAVTA
                     AVEGAAVVVHAAAITNVDACEAEPEAAAAVNDGGTRLVADAARAAGARVLYLSTDYVF
                     SGDAAPYGEDDTTGPLNVYGRTKLAGEGHLDIERDLVVRTSWVFGDGRNFVRTILAAP
                     PPLRVVDDQVGRPTSAAALARALAALVGRPEVTGRLHVAGDGQPCSWADLAAAALAVA
                     GRDVRVERVDSASYAAGAGRVIAPRPPDSTLQLTRARELGLPLEDWRDSVAAYVRGIA
                     "
     gene            2800..3534
                     /locus_tag="VEV43_15475"
     CDS             2800..3534
                     /locus_tag="VEV43_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYP24967.1"
                     /translation="MKGVVLAGGLGTRWLPVTRVINKHLLDVFDEPMIFFPIRSLVAA
                     GISDIVLVTGQEIDQFQTLLGDGSELGARISYAQQAGELGIADALAKAEPFVGSDRMV
                     VILGDNIFQEDLTEYVDAYRRQESGAKLLLKRVALEDARRFGVVTLSDDRIVAVTEKP
                     ADPPSDLVVTGCYMYDARVFDIVRGLEPSGRGELEITDVNNAYVRTGDLTHDVLRGWW
                     TDAGTPPSKLKASILVALAKGVTFHA"
     gene            complement(3541..4710)
                     /locus_tag="VEV43_15480"
     CDS             complement(3541..4710)
                     /locus_tag="VEV43_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYP24968.1"
                     /translation="MSRVVITMPAYRAEATVEKTVAAIPPGTADELILVDDASPDRTA
                     EIARSLGIKVHVHPQNRGYGGNQKTCYTMALESSADVVVMLHPDYQYEPKSVPLLIAP
                     ILAGEADMTFGSRFAGLGDPMAGGMPVYRFVGNRLTTTLENVLLGSRFSEMHSGMRAY
                     TRRCLLSLPFLGYSEDFGFDTQLMVDAVTSGLRVVEVPIPTRYTKESSSISIVKSLKY
                     VAHSLVHTSRQVARRGRRGRRYPVARARALPPSGGPSSTTVRGRVDTEAALALLRSYV
                     LPGGRVLQVDAVASVVDAPLPPGWSGLGARAVAVGAECDVGIVLESNGDLAPLRSIAA
                     HLSDSGAVVVPASRSKGDAVAAAVALRAAGFEMVQWEPPPDETSSALAVARPLPG"
     gene            4788..5939
                     /locus_tag="VEV43_15485"
     CDS             4788..5939
                     /locus_tag="VEV43_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24969.1"
                     /translation="MRAAGGERQRAETVSLWCAIVTLLVAAVPVAIAVDKAGGKPSIL
                     VRMEASEPMANFARTADPEFAFVNDAHYDGVYFYAIATDPFATGEAHTLIDKSAYRYG
                     HAGYGWLGWGASFGQTRAVPVALLLLGFAGLAIAAIAVSRLSAELGWTPWGGLAVAFN
                     PGLVYALTALTSETVGAAFTALALLFWVRRRLVWGAFAAAAACLVKEPFLLVPAGLAL
                     WELVRWLSHRDLGTAVRRIALLSIGPALFACWYVYIRMQFGEWSFEATEGFFALPPFG
                     WLEAIGVASTMAAGADLESQLGAASAPLLAAVGAALIVGLVQAVRVRNAVGAVFVLVA
                     LLALALRPLGVLFPKDMIRELSIPLMLLPLVLAAKPGRDDEEPAAETGA"
     gene            5981..6499
                     /locus_tag="VEV43_15490"
     CDS             5981..6499
                     /locus_tag="VEV43_15490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYP24970.1"
                     /translation="MRTDEELITAMADGDRSALELLYRRHSPWLAGRLARATSSRDLA
                     EEALQDCFVAAWRNPGSFRGEGDVGAWLWAIARRRLASLARKRIDVPAPVTDDVLGDA
                     ADQQALANLDAARVRAAVDRLPAEQRDVMERVVFRGQRLVDVARCLGVPIGTLKSRLH
                     RARQQIRAEMDA"
     gene            6496..>6675
                     /locus_tag="VEV43_15495"
     CDS             6496..>6675
                     /locus_tag="VEV43_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24971.1"
                     /translation="MNDHPREILPLIAEGLRPPEEVRAHLAECDSCAAAVEALSPLDL
                     GYAWEGIAAEVHAPLP"
BASE COUNT          915 a         2384 c         2442 g          934 t
ORIGIN      
        1 gggcgtgcgg ccggacgaag ccgcttcggg ctcctccggc ggggcgtgcg cgcccgcagg
       61 gcggagccgg gcgaggccct cccggtagac ggtcgtcccc gagcaggcgt tgtcgctgaa
      121 gacctcgccg gacccggcat acggcaagac gaggtaggtc tcgccgcggc gataggtccg
      181 ctcgacgctc gacgacgcgc tgatgggtcc cggagggtcc tcggggccgc cgcgcacctc
      241 gacctccgcg gcgacgtctc ccttccacac gtcggagacc gcgacggtcg cccaccggcc
      301 ctcgctggac gtatcggtga cggtcccggc gaagacgaca gaggtctcgg cgatcgcccg
      361 ccccaacgac ggcggttccg cgcacagggc ggacgccggt tgcgaccaca cgagggccaa
      421 ggcgatcccg gcgaacagcg cggcggctcg tctcacggaa cgttcgacgg cggggccggg
      481 ccgatggttc cggccgacgc gcccgcaccg accgcgtagc atgcgccggg tgcaagggcg
      541 gatcgtggtc accggcgggc tgggcttcat cgggtccgcg gtcgtcaggc gactgctcgg
      601 caagggcctc gaggtgacga acctcgacgc cggcacctat gcggcggacc ctcgcaggct
      661 cccgccggac gtcgacacga agcgtgtcga cgtggcggcc gaccccctcg acgagcatct
      721 cgccggtgcg cgggtcgtcg tccacctcgc tgccgagacg cacgtcacgc gcagcgagac
      781 cgacgagagc gccttcttcc ggacgaacgt cgacggcacg cggacggtgc tcgccgcagc
      841 cgagagagcg ggggccgagc tgctcgtcca cttctcgacg gacgaggtct acgggcccgc
      901 tctggaggcg ccgttcgtcg aagccgacaa ggagcccggg gagggcctcg cgacgagcgc
      961 gtacgcgcgt tcgaaggccc tcgccgacga cctcgcgctc ggctacgaag gcgacgtgcg
     1021 cgtgctcgtc gtccggccca cgaactgctt cgggccgtgg cagcaccccg agaaggcgat
     1081 cgcccgatgg accgtgcgcg ccctgtcggg tcaggacctc ccggtgtggg gtgacggcgg
     1141 ctacgtgcgc gactggatgt tcgtcgacga cgtgtgcgac gcggtcgagg tgctggtcga
     1201 cgagcgtcct gcccccggcg cctacaacgt cggcccggag ggcgatcccg tgagcaacgt
     1261 cgagatagcc cgggacattg cccgcctcgc cgggcgcggc gacgacgcgg tctacctcac
     1321 cgcgtacgac cgtccctcgc acgaccgccg ctattcggtc gactcgtcga agatgcgcgc
     1381 gctcgggtgg cggccgcgtg tcggcgtcag ggacgggctc cgcgcgacgg tcgactggta
     1441 ccgcgctcac cgtgactggt gggagccgct ggtggccgag gcagaatcgc tctacgacga
     1501 ccgggtcccg cggtgacctc catcgacggc gtcgtcgtca cacccgtcga gcgccacgag
     1561 gacgggcgcg gcttcttctc cgagatcgcg cgcttcggcg agtaccgcca ggcgaaccac
     1621 tcgcactcgc agcggggcgt gctgcgcggc ctccactacc acctgagcca ggccgacctc
     1681 tggtacgtgg tgcgggggac ggcgcaggtc gcgctcgccg acctccgcgg cgacgagccg
     1741 gtcgtcgaga cgatcacggt cggcgaatcg gcgccgtgca cgatctacgt gccgccgcgc
     1801 gtcgcgcacg gcttcctcgc gctcaccgac gtggacctcg tctacctcgt cacgcaggag
     1861 tacgacgcga gcgacgagca cggcgtggcg tgggacgacc cgacgctcgc gatcccgtgg
     1921 gcgctcgagg acccgatcct gtcggacagg gaccgctcga acccgcaact ggatggttag
     1981 cgggagggtc ctcgtcaccg gcggcgacgg ccaggtgggg gccgcgctcc gcgcgctgct
     2041 cccccacggc gacttccgct cgcacgacga gctcgacgtc accgatgcgg ctgcggtgac
     2101 cgcggccgtg gagggggccg cggtggtagt ccacgcggcg gcgattacca acgtcgacgc
     2161 gtgcgaggcc gagcccgagg cggccgccgc ggtcaacgac ggggggacgc gtctcgtcgc
     2221 cgacgcggcc cgcgccgccg gcgcgagggt cctgtacctc tccaccgact acgtcttctc
     2281 gggcgacgcc gcgccctacg gcgaggacga cacgaccggc cccctcaacg tctacgggcg
     2341 cacgaagctc gcgggcgagg gccacctcga catcgagcgc gacctcgtcg tccgcacctc
     2401 ctgggtcttc ggcgacgggc gcaacttcgt acggacgatc ctcgccgcgc cgccgcctct
     2461 gcgagtcgtc gacgaccagg tcgggcgccc cacgagcgcg gccgcgctcg cgcgggccct
     2521 ggccgcgctc gtcggccggc cggaggtcac cggacgacta cacgtcgccg gcgacggaca
     2581 gccgtgctcg tgggccgacc tcgcggccgc ggccctcgcc gtcgcgggcc gggacgtgcg
     2641 ggtggaacgc gtcgactccg cgtcgtacgc cgcgggggcc gggcgggtga tcgcgccgcg
     2701 gcccccggac agtacgttgc agctgacgcg ggctcgggag ctcgggcttc cgctcgagga
     2761 ctggcgggat tcggtcgcgg cttacgtgag ggggatcgct tgaagggcgt cgtgctggcg
     2821 ggtggtctcg ggacgcggtg gctgccggtg acgcgcgtga tcaacaagca cctgctcgac
     2881 gtcttcgacg agccgatgat cttcttcccg atcaggtcgc tggtcgcggc cgggatcagc
     2941 gacatcgtcc tcgtcaccgg acaggagatc gaccagttcc agaccctcct cggcgacggc
     3001 tcggagctgg gcgcccggat ctcctacgcg caacaggccg gcgagctcgg gatcgcggac
     3061 gcgctcgcga aggcggagcc gttcgtcgga tcggatcgga tggtcgtgat cctcggggac
     3121 aacatcttcc aggaggacct caccgagtac gtcgacgctt accggcggca ggagtccggc
     3181 gccaagctgc tgctgaagcg ggtcgcgctc gaagacgccc gccggttcgg cgtcgtcacg
     3241 ctctcggacg accggatcgt cgcggtgacc gagaagccgg ccgaccctcc gagcgacctg
     3301 gtcgtcaccg gctgctacat gtacgacgcg cgggtgttcg acatcgtgcg cgggctcgag
     3361 ccctcgggac gcggcgagct cgagatcacc gacgtcaaca acgcgtacgt ccgcaccggc
     3421 gacctcacgc acgacgtgct gaggggttgg tggaccgacg cggggacacc gccgagcaag
     3481 ctgaaggcgt cgatcctcgt ggcgctcgcg aagggcgtca ccttccacgc ctgacggggc
     3541 tcagcccgga aggggacgcg ccacggcgag cgccgacgag gtctcgtcgg gcgggggctc
     3601 ccactggacc atctcgaagc ccgcggcgcg caaggccacg gccgcggcga cggcgtcgcc
     3661 cttggaccgg ctcgccggga ccacgactgc ccccgagtcc gagaggtggg ccgcgatcga
     3721 ccgcagcggc gctaagtcgc cgttcgactc gagcacgatc ccgacgtcgc actcggcccc
     3781 cacggcgacg gcgcgggctc cgaggccgct ccaccccggc ggcaacggcg cgtcgacgac
     3841 gctcgcgacc gcgtcgacct ggaggaccct gcctccgggc aggacgtacg aacgaaggag
     3901 cgcgagcgcg gcctcggtgt cgacacgccc gcggaccgtc gtgctcgacg gcccgccgga
     3961 cgggggcagt gcgcgcgcac gggcgaccgg gtagcgacgc ccccggcggc cgcggcgcgc
     4021 gacctgccgc gacgtgtgga ccaggctgtg cgcgacgtac ttcagcgact tgacgatgct
     4081 gatcgacgac gactccttcg tgtagcgcgt cgggatcggc acctcgacga cgcgcagccc
     4141 cgacgtcacg gcgtcgacca tcagctgggt gtcgaacccg aagtcctcgg agtacccgag
     4201 gaacggcagc gacagcaggc accggcgcgt gtaggcgcgc atcccgctgt gcatctccga
     4261 gaagcgcgag ccgagcagca cgttctcgag cgtcgtcgtc aggcggttgc cgacgaaccg
     4321 gtacacgggc atcccgcccg ccatcgggtc gccgagcccg gcgaagcgcg acccgaacgt
     4381 catgtccgcc tcgcccgcga ggatcggcgc gatcagcagc ggcacggact tcggttcgta
     4441 ctggtaatca ggatggagca tgacgaccac gtcggccgag ctctcgagcg ccatcgtgta
     4501 gcaggtcttc tggttgcccc cgtagcccct gttctgaggg tggacgtgga ccttgatgcc
     4561 gagcgaccgc gctatctcgg cggtgcggtc cgggctcgcg tcgtcgacga ggatcagctc
     4621 gtccgcggtc cccggtggga tcgccgcgac ggtcttctcg accgtcgcct cggcccggta
     4681 cgcgggcatg gtgatcacga ctcgggacat tcgttcgatt cctcgtgaag ggtcgcctag
     4741 gggtcacaag gtatcgaacc ggccgggggg cgcgacaatg cagcctcatg agggcggcgg
     4801 gaggcgagag acagcgggcc gagaccgtgt cactttggtg cgcgatcgtg acgctgctcg
     4861 tcgcggccgt tcccgtcgct atcgccgtcg acaaggcagg gggcaagccc agcatcctcg
     4921 tccggatgga ggcgagtgag cccatggcga acttcgcccg gacggccgac ccggagttcg
     4981 ccttcgtcaa cgacgcgcac tacgacggcg tctacttcta cgcgatcgcg acggacccct
     5041 tcgctaccgg cgaggcccac acgctgatcg acaagtccgc ctaccggtac ggccacgccg
     5101 gctacggctg gctcggatgg ggcgcgagct tcggccagac gcgtgccgtg ccggtggcgt
     5161 tgctgctgct ggggttcgcg ggcctggcga tcgcggcgat cgcggtcagc aggctctcgg
     5221 cggagctcgg ctggacgccc tggggcgggc tcgcggtcgc gttcaacccg ggtctcgtct
     5281 acgcgctgac cgcgctcacg agcgagaccg tcggcgccgc cttcaccgcc ctggccctgc
     5341 tgttctgggt gcggcgaagg ctggtgtggg gcgcgttcgc ggccgcggcc gcatgtctgg
     5401 tcaaggagcc gttccttctg gtgccggcgg gcctcgcgct gtgggagctc gtgcggtggc
     5461 tgtcgcaccg cgacctcggc accgccgtgc ggcggatcgc cttgctgtcg atcggccccg
     5521 cgctgttcgc gtgctggtac gtgtacatcc ggatgcagtt cggtgagtgg tcgtttgagg
     5581 cgaccgaggg gttcttcgct ctcccgccgt tcgggtggct cgaggcgatc ggcgtcgcgt
     5641 cgacgatggc tgcgggcgcg gacctcgagt cgcagctggg cgcggcgagc gctcccctgc
     5701 tcgcggcggt gggggccgcg ctgatcgtcg ggctcgtcca agcggtgcgc gtacgcaacg
     5761 ccgtgggtgc cgtcttcgtg ctcgtcgccc tgctcgcgct cgcgctgcgc ccgctcggcg
     5821 tcctgttccc gaaggacatg atccgcgagc tctcgatacc gctgatgctc ctcccgctgg
     5881 tcctggcggc caagcccgga cgggacgacg aagaacccgc cgccgaaacc ggggcctgac
     5941 gggaaccttc ggggcacccg cggcgacaac cccctcgacg atgcggacgg acgaggagct
     6001 gatcaccgcc atggccgacg gggaccgaag cgctctcgag ctgctgtacc ggcgccactc
     6061 accgtggctc gccggcaggc tcgcccgcgc gacctcgtcg cgggacctcg cggaggaggc
     6121 gctgcaggac tgcttcgtgg cggcctggcg caaccccggc tccttcagag gggagggcga
     6181 cgtcggcgcg tggctgtggg cgatcgcgcg ccggcgcctc gcctctctcg cccgcaaacg
     6241 gatcgacgtg cccgccccgg tgaccgacga cgtcctcgga gacgcggccg accagcaggc
     6301 gctcgcgaac ctcgacgccg cacgcgtgcg cgcggcggtc gaccggctgc ccgccgaaca
     6361 gcgcgacgtg atggagcgcg tggtcttccg cgggcagagg ctcgtcgacg tcgcgcgctg
     6421 cctcggcgtc ccgatcggga cgctcaagag ccggctgcac cgcgcccggc aacagatccg
     6481 cgcggagatg gacgcatgaa cgaccacccc cgggagatcc tgccgttgat cgcggagggg
     6541 cttcgacccc ccgaagaggt gcgcgcccac ctggcggagt gcgactcctg cgcggccgcg
     6601 gtcgaggctc tgtcgccgct agacctcgga tacgcgtggg agggcatcgc cgccgaggtt
     6661 cacgcgccgc tcccg
//
LOCUS       DASQDO010000565         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_547376, whole genome shotgun sequence.
ACCESSION   DASQDO010000565 DASQDO010000000
VERSION     DASQDO010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547376"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEV43_15500"
     CDS             <1..203
                     /locus_tag="VEV43_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24972.1"
                     /translation="SGGKGKAAKTDSSGPVQPAMDAPEPAAVPAAVGAASDSSAELEN
                     EGPWLLDMVTDDAGDKEPDPTK"
     gene            224..2581
                     /locus_tag="VEV43_15505"
     CDS             224..2581
                     /locus_tag="VEV43_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24973.1"
                     /translation="MANASSPAAESDQMRAWRALVDSIKAGEAPLGERLYLLGAGPVP
                     VEGAAGGAITLDARGGVVLVVGLAEASATAAGQIARQLTALGGLPGSKLREAGVAPDA
                     GGGLAARHAAWFDLSEPAELNSAQRCVLVVGAEPPDDARAAVEAELGDSLAGLYLTAG
                     EGVTSLTTGAEPEVAEAPEVVEEPEAPEEPEAEIVLEPETPPEAEEPEAEAPAEAETA
                     VEPPVEGEAPVEDEASMDVAAPVEGEAPVTNETTVDATPVETEPEAEAPADELEAPGE
                     GEAPVEDQAGETEILDAETAGAEDADAPAEPEGPGSIEVALDDAPHTGTALDDDAAHD
                     GTAQDEAAPDLVAPYLQDETSTIIVEESDLAEAKELQPAGRFAGWPVGNWIGLTMILV
                     GIGIGIIGVISLRDSSDETARPEPNEQISTVATGVTPDATHARWIGQQRVVTLSDGTM
                     VFVYPTEESLNLVKDGASSGATWEEPFVLDEVVGAKSLSVDVDSKDRLHVAYSDGDSV
                     NYVRLKNKPEQWKPSRLIQIDDDTTDLHVDVAWDEQNQTAHVVWVQQSTEGDAPAYAA
                     LTSEGGIHLTDEGVLTNTGQEVPPLVSVAADGRSSLLVTFRRADQTTGWSSRYSPGPS
                     EDGTFEFFEEEELPTESFVGATDVVYDRQRTAHLVLREDDSANLTYYRKTEAEPWTTS
                     EIALDGDRIEDVDFPTLTYNAEENNLYLFFQTEQYDPAGEISYLVRPLDGTGWQGPFV
                     ITTPVDVPEGALYPVAPDRVSVQALVFWTKTGDPYEIDAAPVRAP"
     gene            complement(2583..>5662)
                     /locus_tag="VEV43_15510"
     CDS             complement(2583..>5662)
                     /locus_tag="VEV43_15510"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HYP24974.1"
                     /translation="VAALYALGGVLLLVKPERTTRSEAGGAALLALAFATKPQTAFLL
                     PVAALVLGFRHFRAKGEPRDLALRLGIPVGTFFAVWFALALPFGLTPGPLLGFYADAG
                     STYPYTSVWAFNLWGLAGFWRADSGPDPVQIFGLPAVAVGILAFCVGASFVLGKAFRA
                     LSQRRPEAEVLLAAGGAIVCLSFALLTRIHERYLFLGLVCLAPLIVYPRLRALFGALS
                     LLYMVNLWFPWVFYVEQAGLTTLNVRGLFDAVYGTVQDSAQKKLLSIVTAAACVFLAV
                     RIWDLLKGAAAAPATVREPAFTRPAGPWKMGLHPIGRKGAALAGAVFLVVVPTRLIGL
                     ASPQEMYFDEIYHARTAGEYLAGRDAYEYTHPPLGKELMALSIATFGRWDVERGEDPP
                     AGLVGASVDSDGTTAAWASPSAAGDGGVLWTATTGDGCTLSSVADGIPLDVEPTAVAL
                     APGGAFVAGVSGQRFVVARYSGNVKQWQTRVDGRVVDLAGSGEVAFAVDEDRKLWRVD
                     SAGAGALSEGAVAVAADPAAAADAALAGIWVAFPDQRVVSAYDSQGTLVASPATEQGA
                     DDVIVVPESDRVVAFDADTGLLETIDSQSRVWERSLDTNGERIAATPRQGIVYAFDGR
                     SVEMVEPRALAIIGDAELPFDAEAFVPLTESGALLAAGSGESACIAGNAFFAWRLPGA
                     LLGALVPALVFLLALRCTGNSLAGLLAAAFVALDGLGFAMARIATLDSQATAQIMAAW
                     VAFASTYFHAHALARGDRRGSMRLGRLWLGATGLFLGLAIATKWVGVYSFLLIGAVVV
                     ADLIVRRERGIGVLFPRFGVAVPAIVAIVVALPLAVYVASYAPYMSLGNSFGDVLRLQ
                     KAMFDYHRNLTADHPYASSWYGWPLGHKAVAMWTGAQGTETGAITAIANPIVLIGGLW
                     GLGVVAFAAWRERVLAYALLPVAALAQYLPWIVVSRAAFLYHYLPVVPFLAIGLASAL
                     LGREGDSRLRRYEAGLVLAAAVAVFTVTLPELDGWFVSPQFHESLHGWFPWLF"
BASE COUNT          945 a         2066 c         2019 g          632 t
ORIGIN      
        1 gttccggggg gaagggcaag gccgcgaaga ccgactcctc cgggccggtc cagccggcga
       61 tggacgctcc cgagccggcc gcggtgccgg ctgcggtcgg ggccgcctcg gactcgtccg
      121 cggagctcga gaacgagggg ccctggctcc tcgacatggt gaccgacgac gccggcgaca
      181 aagagccgga ccccaccaag tagctccccc acacccataa ccgatggcaa acgcttcgtc
      241 tcccgcggcc gagagcgacc agatgcgcgc ctggcgcgct ctcgtcgact cgatcaaggc
      301 cggtgaggct cctctcggcg agcggctcta cctgctcggc gccgggcccg tcccggtcga
      361 gggtgcggcc ggcggcgcga taacgctcga cgcccggggc ggcgtcgtgt tggttgtcgg
      421 gctggccgag gcatcggcga cggccgcggg ccagatcgcc cgccagctga cggcgctggg
      481 cggcctcccg ggatcgaagc tgcgcgaggc cggggtcgcc ccggacgcgg gcggcggcct
      541 ggcggcccgc cacgcggcgt ggttcgacct atcggagccg gccgagctca actcggccca
      601 gcgttgtgtg ctggtcgtgg gcgccgagcc gccggacgac gcccgggcgg ccgtggaggc
      661 cgagctgggc gactcgctgg cgggtctcta cctgacggcg ggcgagggcg tgacgtcgct
      721 gacgacgggc gccgagccgg aggttgccga ggcgcctgag gttgtcgaag agcccgaggc
      781 tccggaggag cccgaggccg agatcgtcct cgagcccgag accccgccgg aggccgagga
      841 gcccgaagcc gaagcccccg cagaggccga gaccgcggtc gagccccccg tcgagggcga
      901 ggcgcccgtg gaggacgaag cctccatgga cgtcgccgct ccggtcgagg gcgaggcccc
      961 cgtcacgaac gagaccacgg tcgacgccac cccggtcgag accgagcccg aagccgaggc
     1021 tcccgccgac gagctcgaag ctcccggcga gggcgaggct cccgtcgagg accaggccgg
     1081 cgaaactgaa atcctcgacg ccgagacggc cggcgcagaa gacgcggacg ccccggccga
     1141 gccggagggg cccggctcca tcgaggtcgc cctcgacgac gccccccaca ccgggactgc
     1201 cctcgacgac gacgccgcac acgacggcac ggcccaggac gaggcggccc ccgacctcgt
     1261 cgctccctac ctccaggacg agacctcgac gatcatcgtc gaggagagcg acctcgccga
     1321 ggccaaggag ctccagcccg ccgggcggtt cgccgggtgg ccggtgggga actggatcgg
     1381 gctcacgatg atcctcgtcg ggatcgggat cggcatcatc ggcgtgatca gcctcaggga
     1441 ctccagcgac gagacggctc ggcccgagcc caacgagcag atcagcacgg tcgccaccgg
     1501 cgtgacgccg gacgccacgc acgcccgctg gatcggccag cagcgcgtcg tgacgctcag
     1561 cgacggcacg atggtgttcg tctatccgac ggaggagtcg ctcaacctcg tcaaggacgg
     1621 ggcctcgagc ggcgccacct gggaggagcc cttcgtcctc gacgaggtcg tcggcgcgaa
     1681 gtccctgtcg gtcgacgtcg actcgaagga ccgcctgcac gtcgcgtaca gcgacggcga
     1741 ttccgtcaac tacgtccggc tgaagaacaa gcccgagcag tggaagccga gccggctcat
     1801 ccagatcgac gacgacacga ccgacctcca cgtcgacgtc gcgtgggacg agcagaacca
     1861 gaccgcgcac gtcgtctggg tccagcagag caccgagggc gacgcccccg cgtacgccgc
     1921 gctgacgtcg gagggcggca tccacctgac cgacgaaggc gtcctgacga acacgggcca
     1981 ggaggtcccg ccgctggtct ccgtcgcggc cgacggacgc tcgtcgttgc tcgtgacgtt
     2041 ccgccgcgcc gaccagacga ccggatggtc gagccgctac tcgccggggc cgtcggagga
     2101 cgggacgttc gagttcttcg aggaggagga gctgccgacc gagtcgttcg tcggcgctac
     2161 cgacgtcgtc tacgaccgcc agaggaccgc acacctcgtc ctgcgcgagg acgactccgc
     2221 gaacctcacc tactaccgca agaccgaagc agagccgtgg acgacgtccg agatcgcgct
     2281 cgacggcgac aggatcgaag acgtcgactt cccgacgctg acctacaacg cggaggagaa
     2341 caacctctac ctcttcttcc agaccgagca gtacgacccg gccggcgaga tctcttacct
     2401 cgtgcgcccg ctcgacggga cgggatggca ggggccgttc gtcatcacga cgccggtcga
     2461 cgtgcccgag ggcgcgctct accccgtggc gcccgaccgc gtgagcgtgc aggcgctcgt
     2521 gttctggacg aagaccggcg atccctacga gatcgacgcc gcgccggtga gggcccccta
     2581 gctcagaaca gccacgggaa ccacccgtgg agcgattcgt ggaactgcgg cgacacgaac
     2641 cagccgtcga gctcgggcag cgtcaccgtg aacaccgcga cggcggcggc cagcaccaac
     2701 ccggcttcgt acctcctcag ccgcgagtcg ccctcgcgcc ccagcagggc cgacgccagc
     2761 ccgatcgcga ggaacggcac cacgggcagg tagtggtaca ggaaggcggc gcggctcacg
     2821 acgatccacg gcaggtactg cgccagcgcg gcgaccggca gcagcgcgta ggccaggacg
     2881 cgttcgcgcc acgcggcgaa ggccacgacg ccgaggcccc acagcccgcc tatcaagacg
     2941 atggggttcg cgatcgcggt gatggcgccg gtctcggtcc cctgggcccc cgtccacatc
     3001 gcgacggcct tgtgccccag cggccagccg taccacgacg acgcgtaggg gtggtcggcc
     3061 gtcaggttgc ggtggtagtc gaacatcgcc ttctgcaggc gcagcacgtc gccgaacgag
     3121 ttcccgagcg acatgtacgg cgcgtacgac gccacgtaga cggcgagcgg cagcgcgacc
     3181 acgatcgcga cgatcgcggg gaccgccacg ccgaagcggg ggaagaggac gccgatgccc
     3241 cgctcgcgcc gcacgatcag gtcggcgacc acgaccgctc cgatcagaag gaacgagtag
     3301 acgccgaccc acttcgtcgc gatcgccaga ccgaggaaga gaccggtagc gccgagccac
     3361 agcctgccca gccgcatgct gccgcgccgg tccccgcgcg cgagcgcgtg cgcgtggaag
     3421 tacgtggagg cgaacgccac ccacgcagcc atgatctgcg ccgtcgcctg gctgtccagc
     3481 gtcgctatgc gcgccatcgc gaagccgagg ccgtcgagcg cgacgaaggc ggccgccagc
     3541 aggccggcga gggagttgcc ggtacaacga agcgcgagca ggaagaccag cgccggtacg
     3601 agcgcgccga gcagcgctcc cgggagccgc cacgcgaaga acgcgttgcc ggcgatgcac
     3661 gcgctctcgc ccgagcccgc ggcgagcagg gcgccactct cggtcagggg cacgaacgcc
     3721 tccgcgtcga acgggagctc ggcgtcgccg atgatcgcga gagcgcgcgg ctcgaccatc
     3781 tcgacggagc ggccgtcgaa ggcgtagacg atcccctgcc gcggcgtcgc cgcgatccgt
     3841 tcgccgttgg tgtcgagcga ccgctcccac acgcggctct gactgtcgat cgtctcgagg
     3901 aggccggtgt ccgcgtcgaa cgcgacgacg cggtccgact cggggacgac gatcacatcg
     3961 tcggcaccct gctcggtcgc ggggctcgcg acgagggtcc cctgcgagtc gtacgccgag
     4021 acgacccgct ggtcggggaa cgcgacccag atgcccgcca gcgcggcgtc cgccgccgcg
     4081 gcagggtcgg cggcgacggc gacggcgcct tccgacagcg cgccggcgcc cgcggagtcg
     4141 acccgccaga gcttgcggtc ctcgtcgacg gcgaacgcca cctcgcccga gcccgcgagg
     4201 tcgacgacgc gtccgtcgac gcgcgtctgc cactgcttca cgttccccga gtagcgcgcg
     4261 acgacgaacc gctggcccga gacgccggcg acgaacgcgc ccccgggcgc gagcgcgacc
     4321 gcggtcggct cgacgtcgag cgggatgccg tcggctaccg acgagagcgt gcagccgtcc
     4381 ccggtcgtgg ccgtccacag gacgccgccg tcgcccgcgg cggagggcga ggcccacgcc
     4441 gcggtcgtgc cgtcggagtc gaccgaggca cccaccaggc ccgccggagg atcctctccg
     4501 cgctcgacgt cccaccggcc gaacgtcgcg atcgagagcg ccatcagctc cttgccgagc
     4561 ggcgggtgcg tgtactcgta cgcgtcgcgg cccgcgaggt actcgcccgc ggtacgtgcg
     4621 tgatagatct cgtcgaagta catctcctgc gggctcgcga gcccgatcag ccgcgtcggc
     4681 acgaccacga ggaacacggc gcctgcgagc gcggccccct tgcgcccgat gggatgcagg
     4741 cccatcttcc acggccccgc cggtcgcgtg aaagccggct cgcgcacggt cgccggcgcc
     4801 gccgcggccc ctttcaagag gtcccagatg cgcacggcga ggaacacgca cgccgccgcg
     4861 gtcacgatgc tcaagagctt cttctgcgcg ctgtcctgga cggtcccgta gacggcgtcg
     4921 aagagcccgc ggacgttcag cgtcgtcagg cccgcttgct cgacgtagaa gacccacggg
     4981 aaccagaggt tgaccatgta gaggagcgac agcgcgccga acaaggcgcg cagacgcggg
     5041 tagacgatca acggggcgag gcacacgagg ccgaggaaca ggtagcgctc gtggatcctc
     5101 gtcagcagcg cgaacgacag gcagacgatg gcgccgccgg ccgcgagcag gacctcggcc
     5161 tcggggcgcc tctgcgacag cgctcggaag gccttcccga ggacgaacga ggcccctacg
     5221 cagaacgcga ggatgccgac cgccacggcc gggagcccga agatctggac cgggtccggc
     5281 cccgagtccg cacgccagaa ccctgcgagc ccccacaggt tgaaggccca cacgctcgtg
     5341 tacggatagg tcgaccccgc gtcggcgtag aagccgagca gcggccccgg cgtcagcccg
     5401 aacggcagag cgagcgcgaa ccagacggcg aagaaggtcc ccacggggat gccgagccga
     5461 agcgcgaggt cgcgcggctc ccccttggcc cggaagtgcc ggaacccgag gaccagcgcc
     5521 gccaccggca ggaggaacgc ggtctgcggc ttcgtcgcga acgcgagggc cagaagggcc
     5581 gcgccgccgg cctcggagcg ggtcgtgcgc tccggcttca ccagcagcag cacgcctccg
     5641 agcgcgtaca gggccgcgac gg
//
LOCUS       DASQDO010000566         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_547392, whole genome shotgun sequence.
ACCESSION   DASQDO010000566 DASQDO010000000
VERSION     DASQDO010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547392"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VEV43_15515"
     CDS             complement(<1..574)
                     /locus_tag="VEV43_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYP24975.1"
                     /translation="MIACRDCGTENPEVATFCRNARCGAFLGWHSAGDAATAPLPVAR
                     EVEDVDRVQELFVGVAPRGTARADPAGTPGVDLWVHPAAVEVEPGAPALIELRVHNSG
                     TVVDRITISVEGDAAPWATVVVGGPAEAAPRASGDANLYPGDGASATISLDPPRDPPA
                     RAGTVRLVVRARSSEDPAVSATRHVTVVVRR"
     gene            complement(571..1110)
                     /locus_tag="VEV43_15520"
     CDS             complement(571..1110)
                     /locus_tag="VEV43_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYP24976.1"
                     /translation="MSPGRGPAPGLATPHPLGALLPSVYQDDDFVQRFTAGLDDVLAP
                     VLSVLDCLDAYVDPALAPEDFVAWLEGWAGLERDEDVPLERRRAMVASATAAYRMHGT
                     ARGIAATVRALTGAEPAVRDTGGATWSVSPVAAPPEPAAPEVVVTVPPGLDVDLRASD
                     RAVAAAKPAHVPHRVEVAP"
     gene            complement(1107..>2638)
                     /locus_tag="VEV43_15525"
     CDS             complement(1107..>2638)
                     /locus_tag="VEV43_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HYP24977.1"
                     /translation="DGRTGRLHDRTALLHAGTGCRCFGDPPVTGDALLVALTAAAPSA
                     TVLLRIECAVEGVGVDPRDPPLRWEAWTGREWTPCEVERDETGGLNRPGDVLLGVPPG
                     HTVAAFGGRAGGWLRCRLVTPGPRQHAYRQSPRIYRIVAGTVGGTTSAVHADVVENEI
                     VGTSEGTPGQRFPLRHSPVVPSGEPPVLEVSTPAGWSEWSQVETFAFSGAGDPHFDLD
                     EAAGEVLLGPAVRELDGTVRHYGAIPPAGATLRLRSYRAGGGSEGNVSAGAISVLKSA
                     IPFVTAVENRRAATGGVAVEDLESAKARGPLVLRTHDRAVTASDYETLTRRVAPEIAR
                     VKCLGPEDAAGAVRVLVVPAVHDDGRGHLDVDALVPLEETLERIARHLDERRPVGTSV
                     RVQPPTYQAVTIVARLRAQRGHSARRIRSDALDHLYRYYHPTTGGPGGDGWPFGRPLH
                     LGEVHGVLQRVPGIDLVEDVRLFPADPVTGERADPVHRLDLDPSTVAYSYEHRVLVED
                     S"
BASE COUNT          335 a          967 c         1031 g          305 t
ORIGIN      
        1 agcgccggac gacgacggtc acgtgccgcg tcgccgacac ggcggggtcc tcggacgagc
       61 gcgcgcggac gacgagtcgg accgtgccgg cgcgtgccgg agggtcccgc ggggggtcga
      121 gcgagatcgt cgcggaggca ccgtcgcccg ggtagaggtt cgcgtcgccg gaagcccgcg
      181 gggcggcctc ggccggaccg ccgacgacga ccgtcgccca cggcgccgcg tcgccctcga
      241 ccgagatcgt gatccggtcg acgaccgtgc cggagttgtg gactcgcagc tcgatcagcg
      301 cgggagcacc gggctcgacc tccactgcgg ccggatgcac ccacaggtcg acccccggcg
      361 tcccagcggg gtcggcgcgc gcagtcccgc gcggggcgac gccgacgaag agctcctgca
      421 cgcggtcgac gtcctcgacc tcgcgggcta ccggcagcgg cgcggtcgcg gcgtcgccgg
      481 cggagtgcca gccgaggaag gcaccacagc gcgcgttgcg gcagaaggtc gcgacctcgg
      541 ggttctcggt accgcagtcg cggcacgcga tcacggcgcg acctccacgc ggtgcggcac
      601 gtgcgcgggc ttcgcggccg ccacggcacg gtcgctcgcg cgcaggtcga cgtcgagccc
      661 cggcgggacc gtcacgacga cctcgggggc cgcgggctcg ggaggtgccg cgaccggcga
      721 gaccgaccag gtcgcgcctc cggtgtcgcg caccgcgggc tcggcccccg taagagcgcg
      781 caccgtagcg gcgatgccgc gggcggtccc gtgcatccgg taggcggcgg tggccgacgc
      841 gaccatcgcg cggcgtcgtt cgagcgggac gtcctcgtcg cgctcgaggc cggcccagcc
      901 ttcgagccac gccacgaagt cctccggcgc gagcgcgggg tccacgtacg cgtcgaggca
      961 atcgaggacc gacaggaccg gcgcgagcac gtcgtccagc cccgcggtga agcgctggac
     1021 gaagtcgtcg tcctggtaga ccgagggcag cagcgcgccc agcggatggg gcgtggcgag
     1081 gcccggcgcg ggcccgcggc ccgggctcac gagtcctcca ccaggacgcg gtgctcgtac
     1141 gagtacgcga ccgtcgacgg gtcgaggtcg aggcggtgga cggggtcggc ccgttcgccg
     1201 gtgacggggt cggcggggaa gagccggacg tcctcgacca ggtcgatccc cgggacgcgc
     1261 tggagcacgc cgtggacctc gcccaggtgc aacggccttc cgaagggcca gccgtctccg
     1321 ccggggcccc cggtcgtcgg gtggtagtag cggtagaggt ggtccagggc gtcggagcgg
     1381 atgcgccgcg cgctgtgccc gcgctgcgcg cgcaagcggg cgacgatcgt gacggcctgg
     1441 taggtcggag gctgcacccg gacgctggtc ccgacgggcc ggcgctcgtc gaggtgtcgc
     1501 gcgatccgtt cgagcgtctc ctcgaggggg acgagcgcgt cgacgtcgag atggccgcgc
     1561 ccgtcgtcgt gcaccgcggg aacgacgagc acgcgcacgg cccccgcggc gtcctccggt
     1621 cccaggcact tcacgcgcgc gatctccggg gcgaccctgc gcgtgagcgt ctcgtagtcc
     1681 gacgcggtca cggcacggtc gtgcgtgcgc agcacgaggg ggccgcgcgc cttcgcggac
     1741 tcgaggtcct cgactgcgac gccgcccgtc gccgcgcgcc ggttctcgac cgcggtgacg
     1801 aacgggatcg cgctcttgag gaccgatatc gcgccggcgg agacgttgcc ctcggaaccg
     1861 cctccggccc ggtacgaccg gagtcgcagc gtcgcgcccg cgggagggat cgccccgtag
     1921 tggcgcacgg tgccgtcgag ctcgcgcacg gcggggccga gcaggacctc gcccgcggcc
     1981 tcgtcgaggt cgaagtgggg gtcgccggcg ccgctgaacg cgaacgtctc gacctgcgac
     2041 cactcgctcc agccggcggg tgtcgagacc tcgagcacgg gcggctcgcc ggagggcacg
     2101 acgggcgagt gacgcagcgg gaaccgttgg cccggcgttc cctcggacgt cccgacgatc
     2161 tcgttctcga cgacgtcggc gtggactgcg gacgtcgtcc cgccgaccgt gcccgccacg
     2221 atgcggtaga tgcgcggcga ctgacggtac gcgtgctgcc gggggccggg cgtgacgagc
     2281 cggcagcgca gccatccacc ggcgcggccc ccgaacgccg cgacggtgtg acccggaggc
     2341 acgcccagca ggacgtcgcc ggggcggttc agcccgccgg tctcgtcgcg ctcgacctcg
     2401 cacggcgtcc actcgcgtcc cgtccatgcc tcccatcgca gcggaggatc gcgcgggtcg
     2461 acgccgacgc cctcgacggc gcactcgatc cgcaggagga cggtcgcgga cggcgccgcc
     2521 gcggtcagcg cgacgagcag cgcgtcgccc gtgacgggag gatcgccgaa gcaccggcac
     2581 cccgtgcctg cgtgtagcag cgcggtgcgg tcgtgcagcc gcccggtccg tccgtcgc
//
LOCUS       DASQDO010000567         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_731968, whole genome shotgun sequence.
ACCESSION   DASQDO010000567 DASQDO010000000
VERSION     DASQDO010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731968"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="mltG"
                     /locus_tag="VEV43_15530"
     CDS             <1..859
                     /gene="mltG"
                     /locus_tag="VEV43_15530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYP24978.1"
                     /translation="KLLEEKGVIESAFGFRIAAYLEGGADDIQAGRYEIRTGLTAKDA
                     LAALVDNPPVAESVSVTFPEGLWLEDFGRILEEQTDLSGERFLRVLEKGKVRSSLLPS
                     GITSHEGLLFPSTYQVGENDDEVDVARKLVGEMEEQVAPLDFSVAESMGYSRYEAIVI
                     ASMIEAEAKLDSERPKVARVIYNRLEIGEKLGIDATVNYAIRDHKLGLTVSELATDSP
                     YNTRLYPGLPPTPIGAAGAEALAAAAEPADGDWLFYVLADCDGRHAFSETYDEFLQDK
                     AVYEALGCS"
     gene            856..1689
                     /gene="aroE"
                     /locus_tag="VEV43_15535"
     CDS             856..1689
                     /gene="aroE"
                     /locus_tag="VEV43_15535"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYP24979.1"
                     /translation="MKQGSGFVGILGWPLEHTLSPLIHNVAFRRTSLDWTYLSWPVEP
                     ALLGDAVRGLRALGAFGANVTMPHKEAVIEHLDEVSGDAREVGAVNTIQRVGDKLIGH
                     NTDVDGFAEFLSGDAGFDATGKRALVLGSGGAARAVVTALESLGASDVTVAGRDPDRT
                     GSVAGLADARPAPWDDAAKLAEDADLVVNATPLGMKGERVLEGIAFAPGQWVVDLIYT
                     PPSTPLIEAARAGGAQAWGGLGMLVRHAAASFTIWTGQAAPLEAMSAAAVHAIGAHRA
                     G"
     gene            1759..>2084
                     /locus_tag="VEV43_15540"
     CDS             1759..>2084
                     /locus_tag="VEV43_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HYP24980.1"
                     /translation="MTKADQLSTALDDFLTISAEVEAAAVVSADGLPMASALPPHVEE
                     DRLAAMSAALLTLGERAASELGKGELAQVFIEGPKGHVILMAAGRDCGLVCVTAKGAK
                     VGLVLY"
BASE COUNT          323 a          698 c          765 g          298 t
ORIGIN      
        1 caagctcctc gaagagaaag gcgtgatcga gtccgcgttc ggcttccgca tcgccgcgta
       61 cctcgagggg ggtgcggacg acatccaggc cggccgctac gagatccgga cggggctcac
      121 cgcgaaggac gcgctcgcgg cgctcgtcga caacccgccg gtggcggagt ccgtgtcggt
      181 cacgttcccc gaggggctgt ggctggagga cttcggccgc atactcgaag agcagacgga
      241 cctctcgggc gagcgcttcc tcagggtcct cgagaagggc aaggtgcggt cgtcgctgct
      301 gccgtccggc atcacgagcc acgaagggct cctgttcccg tcgacgtacc aggtcggtga
      361 gaacgacgac gaggtcgacg tcgcgcgcaa gctcgtcggg gagatggagg agcaggtcgc
      421 gccgctcgac ttctccgtcg ccgagtcgat gggctacagc cgctacgagg cgatcgtgat
      481 cgcgtcgatg atcgaggcgg aggcgaagct cgactccgaa cggccgaagg tggcgcgcgt
      541 gatctacaac cggctcgaga tcggcgagaa gctgggcatc gacgccaccg tcaactacgc
      601 gatccgcgac cacaagctcg gcctgaccgt gagcgagctc gcgaccgact ccccctacaa
      661 cacgcggttg taccccgggc tcccgccgac gccgatcgga gccgcggggg ccgaggcctt
      721 ggcggcggcg gccgagccgg cggacggcga ctggctcttc tacgtcctcg ccgactgcga
      781 cggccggcac gcgttcagcg agacctacga cgagttcctg caggacaagg ccgtctacga
      841 agcgctgggc tgctcgtgaa gcaggggagc ggcttcgtcg ggatcctcgg gtggccgctc
      901 gagcacacgt tgtcgccgct catccacaac gtggcgttcc ggaggacgtc gctcgactgg
      961 acgtatctgt cgtggcccgt cgagccggcg ttgctcgggg atgcggtgcg cggcctccgt
     1021 gcgctgggcg ccttcggcgc gaacgtcacg atgccgcaca aggaagccgt gatcgagcac
     1081 ctcgacgagg tctccggcga cgcacgcgag gtcggcgcgg tcaacacgat ccagcgggtg
     1141 ggcgacaagc tgatcggtca caacaccgac gtcgacgggt tcgcggagtt cctctcggga
     1201 gacgcggggt tcgacgcgac gggcaagcgc gcgctcgtgc tcggctccgg cggcgcggcc
     1261 cgtgcggtcg tgacggcact cgagtcgctc ggagcgtcgg acgtcacggt cgcgggccgg
     1321 gacccggaca ggacgggatc ggtcgcgggg ctcgcggacg cccgaccggc gccgtgggac
     1381 gacgcggcga agctcgccga agacgccgac ctggtcgtga acgcgacccc gctcgggatg
     1441 aaaggcgagc gcgtgctgga aggcattgcg ttcgcgccgg ggcagtgggt ggtcgacctg
     1501 atctacacgc ccccgtcgac gccgctgatc gaagcggccc gggcgggcgg agcgcaagcc
     1561 tgggggggcc tgggcatgct ggtccggcac gcagcggcct cgttcacgat ctggacgggc
     1621 caagccgcgc cgctggaagc catgtcggcc gcggcggtgc acgcgatagg ggcccaccga
     1681 gccggctgat tcaaggtttt ccggggtcct gccgaagcga atgactagcc attcgtgact
     1741 ctgcaggagg actccctgat gaccaaagcg gaccagctct cgaccgcgct cgacgacttc
     1801 ctcaccatct cggcggaggt cgaggcggcc gccgtcgtca gcgccgacgg cctcccgatg
     1861 gcgtcggccc tgccccctca cgtggaggag gaccgcctag ccgctatgag cgcggcgctg
     1921 ctgacgctcg gcgagcgtgc tgcctccgag ctcggcaagg gcgagctcgc ccaggtcttc
     1981 atcgagggcc ccaagggcca cgtgatcctg atggccgccg ggcgcgactg cggcctcgtc
     2041 tgcgtcacgg cgaagggggc caaggtcggc ctcgtcctct acga
//
LOCUS       DASQDO010000568         6828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_609381, whole genome shotgun sequence.
ACCESSION   DASQDO010000568 DASQDO010000000
VERSION     DASQDO010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609381"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VEV43_15545"
     CDS             complement(<1..1406)
                     /locus_tag="VEV43_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYP24981.1"
                     /translation="MRRLDRVGGTATILVTDLVGSTAMFDRLGDESAERLLRAHLGEL
                     RDLVRSFGGVEVKSTGDGIMAAFPAAWLATECAVAMQRALHLRNDRVAGSDDEPLHVR
                     IGLHSGDTTADRHDYYGIAVAVAARLCDKASPDEILVSGVTRGLVGSRGGHAFTDAGV
                     PSWKGSDEPVQAWALEWGPGQLPPATPPGLERRRGPLVAAGAGALLIVGLGAGMLMAS
                     DEEPRRDPPPVAEGRIVRVSVRSNGEETAGASSAAEISGAASHVVFESTADDLVEADT
                     NGAVDVFHVSIAGGLDPKTVELVSRDEDTLGNGASTQPSISDDGNVVGFLSVATNLAG
                     QPDRNGFQDVFMHRVDEGETSRVSVSTTGVAAKGRSFEVDVSGDGDFIAFTTAAENLA
                     VQGDDNRKDDIFVRDVLEGKTNRANIRTEDLKEANKDSSDPSISAEGEYVAFASPADT
                     LSKGDTNNSPDVFRYNRKL"
     gene            1425..2099
                     /locus_tag="VEV43_15550"
     CDS             1425..2099
                     /locus_tag="VEV43_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYP24982.1"
                     /translation="MGPNPAVAGFLARLSEEEERALFEGGRKRRFEAGTTIFHEAEVA
                     DRVVMLLSGRAKIATFTEDGKEVMLGVRDPGDLLGEFSALDGKPTSANVIAMEPIEAL
                     VLDVGSFKSWLTEHPATALLLLETVLARLRDADRKRVEFSAYDTVARVARRLFELAER
                     FGVETDAGVEIKLPMTQDELAAWTGASRVGVSQALKALRDRGWIETRRRSITVLDMEA
                     LKRRAT"
     gene            2253..3563
                     /locus_tag="VEV43_15555"
     CDS             2253..3563
                     /locus_tag="VEV43_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24983.1"
                     /translation="MAVLATLAMATAAIAQPTANTERVSERSNGVEGNDDSFWCAISG
                     DGRFVGFTSRASNLIGADRNDATDVFLFNRETETPEFVSVRSTGTQGNRGSDSPSLSY
                     TGRYVAFATAASNLAIKGDRNGVNDVFVYDRNEGRTNRASIRSDLGEANGSSYDPSIS
                     SNGRFVAFTSSADNLAVKGDNNGREDVFVHDRTTLRTNRVSISSKLKEGNGSSYSPSI
                     SGDGRMVTFVSQANNLVRRDTNRRPDIFVYNRSTRKTIRVSIAGDGTQANGESSAPMI
                     SADGGFVAFHSNASNLIASDTNNRTDVFVHDLSSGETTRISRGMANSEPNGDSFIPAI
                     SADGRYVAFQSTASNLVAGDTNGTPDQYQGLDVFRYDRQTNQTERVSVGDDGEQANGP
                     SNDPGDDPSITADGRYVCFASFASNLVEDDTNGAYDVFVRGPLL"
     gene            3727..5007
                     /locus_tag="VEV43_15560"
     CDS             3727..5007
                     /locus_tag="VEV43_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24984.1"
                     /translation="MCPPFAMPLVGFLAVAPLCLSLSVRPAPAEAAYVGNNGRILFEA
                     RRDGNPEIYVMDADGTNQERLTFSKTWDIAATWSPDGTRIAWGSEEDGDREIFVMNAD
                     GSDQTQLTFNRYYDRAPTWSPDGTKLAFASERNRQYAIYVINADGTGGETRITPPGMS
                     ARHPAWSPDGTKLAFESRRDGNPEIYTSRLNGKGLRRLTHDPANDFVPNWSSDGEHLV
                     FASTRDGNHEIYSVAADGSDERRLTENVFYDSEPNWSPDGSKVVFWSTRHGNIDIYSI
                     NPDGSGETRLTDHPGKDYSPDWQPLCTVAGTSGDDLLTGTLGDDLICGGGGDDTIMGG
                     GGADVIFGGDGDDSISGGPGNDVIAGGEGADDLLGEDGLDRIFGTAGPDTADGGPGAD
                     VVNVKDGDGDDDLDGGGGSDKCVSDSGDGKTNCP"
     gene            5004..5816
                     /locus_tag="VEV43_15565"
     CDS             5004..5816
                     /locus_tag="VEV43_15565"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYP24985.1"
                     /translation="MSASRMRRRLWALVFLQLLVATAALANENETANWVPFTGSYSIS
                     RTFQHYGGHDTPGIDFRIPAGTPIRAAGPGTVVGIEERCKPGHRWCSGRRGNYVQIAH
                     PAGRHSRYLHLRPRGVVVSLGEQVVRGQVIGYSGNTGDSTGPHLHYDELLNGSRIYPG
                     PMYALHGGETVAYRDWPGADERLIRNDGYPLTDLTVAIEGAGRVTSSPAGIDCGPDCV
                     ETYEPGELVVLTAEPDELYTFAGWSGACEGTDPCELRMTSSMTVTATFVLLP"
     gene            5822..6580
                     /locus_tag="VEV43_15570"
     CDS             5822..6580
                     /locus_tag="VEV43_15570"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HYP24986.1"
                     /translation="MRHRVLRGVLVVCSISVAGCTESPPDPTRDEPTAPNSVHVIVDD
                     EARQAAVGVGVDLDAAVAAALRKVAKEIDLPMTTVSVAVRPREVIPEVGIGGYTDHLG
                     DVHVFLDPRRDDLADVLRVWLEPMLAHEAHHAARTVDGPGYGRSLVEAIVSEGLADAY
                     SEEAFPETPALPWTQALEPGAICPWWRRASGERGAYDHEEWFFGAGRAPRWAGYTLGY
                     ALVRRYLRAHATSAADSVRTPARVIVRSADLCRS"
BASE COUNT         1091 a         2448 c         2341 g          948 t
ORIGIN      
        1 agcttgcggt tgtagcggaa gacgtctggg gagttgttcg tgtcgccctt ggacagggtg
       61 tcggccggcg aggcgaacgc gacgtactcg ccctccgcgg agatgctggg gtccgagctg
      121 tccttgttcg cctccttcag atcctcggtc cggatgttcg cgcggttggt cttcccctcc
      181 agcacgtcgc gcacgaagat gtcgtccttg cggttgtcgt cgccctgcac ggcgaggttc
      241 tcggccgcgg tcgtgaacgc gatgaagtcc ccgtcgcccg agacgtcgac ctcgaagctg
      301 cggcccttcg cggcaacgcc cgtcgtcgag acgctcacgc ggctcgtctc gccctcgtcc
      361 acgcggtgca tgaagacgtc ctggaagccg ttgcgatccg gctgcccggc caggttcgtc
      421 gcgacgctga ggaacccgac gacgttcccg tcgtccgaga tcgagggctg ggtgctggcg
      481 ccgttcccga gggtgtcctc gtcgcggctc acgagctcga cggtcttcgg gtcgagccca
      541 ccggcgatcg agacgtggaa gacgtcgacg gctccgttcg tgtccgcctc gacgaggtcg
      601 tcggcggtgc tctcgaacac gacgtgcgac gcggcccccg agatctcggc cgccgagctc
      661 gcgcccgcgg tctcctcgcc gttgctcctg acgctgacgc ggacgatccg gccctcggcc
      721 acgggcggcg ggtcgcggcg aggctcctcg tcgctggcca tcagcatccc ggcccccagc
      781 ccgacgatca gcagcgcgcc cgcacccgcg gccacgaggg ggccgcggcg acgctcgagc
      841 cccggcggcg tcgccggcgg cagctgtccc ggcccccatt cgagcgccca cgcctgcacc
      901 ggctcgtcgc tccccttcca ggacggcacc cccgcgtcgg tgaacgcatg ccccccgcgc
      961 gacccgacga ggccccgcgt cacgccggag acgaggatct cgtcgggcga cgccttgtcg
     1021 cacaagcggg ccgcgaccgc gaccgcgatc ccgtagtagt cgtgccggtc ggccgtggtg
     1081 tcgcccgagt ggagccctat ccggacgtgc agcggctcgt cgtccgagcc ggcgacccgg
     1141 tcgttgcgca ggtggagcgc ccgctgcatg gcgaccgcgc actccgtcgc gagccaggcg
     1201 gccgggaacg ccgccatgat cccgtcgccg gtgctcttga cctcgacccc gccgaaggag
     1261 cggacgaggt cgcgcagctc gcccaggtgg gcgcgcagca gccgctccgc gctttcgtcc
     1321 ccgagccggt cgaacatcgc cgtggagccg actaggtcgg tgaccaggat cgtcgcggtg
     1381 ccgcctacgc gatcgagacg cctcacgccc ataaggtaaa ggagatgggt ccgaacccgg
     1441 ccgtggcggg cttcctggcg cggctctccg aggaagagga gcgcgcgctc ttcgagggcg
     1501 ggcgcaagcg ccgcttcgag gcgggcacga cgatcttcca cgaagccgag gtcgcggacc
     1561 gcgtcgtgat gctgctgagc ggccgcgcca agatcgcgac gttcacggag gacggcaagg
     1621 aggtgatgct cggcgtccgc gacccgggcg acctcctcgg cgagttctcg gcactcgacg
     1681 gcaagccgac ctccgccaac gtgatcgcga tggagccgat cgaggcgctg gtcctcgacg
     1741 tgggctcgtt caagtcctgg ctcaccgagc atccggcgac cgcgctgctg ctgctcgaga
     1801 ccgtcctcgc ccgtctgcgc gacgccgacc gcaagcgcgt cgagttctcc gcttacgaca
     1861 ccgtcgcccg ggtggcccgg cgcctgttcg agctcgccga gcggtttggc gtcgagaccg
     1921 acgcgggcgt cgagatcaag ctcccgatga cgcaggacga gctcgccgca tggaccggcg
     1981 cgtcgcgcgt cggcgtgagc caggccctca aggcgctgcg cgaccggggc tggatcgaga
     2041 cccggcgccg ttcgatcacg gtgctcgaca tggaagcgct caagcgccgc gccacctgac
     2101 gaacgttcag gatttagcac tcccttcgtc aagagctgag tactcgcgcg acctcctttt
     2161 ctcgaccatg ggatcacgag ccggcaacag agccggcggc ggatcaccca aaggagggtc
     2221 ctatgacctc gaagtggact atcaggggcg ccatggccgt cctggccacc cttgcgatgg
     2281 cgacggcggc gatcgcgcag cccaccgcga acacggagcg cgtaagcgag cgctcgaacg
     2341 gcgtcgaggg caacgacgac agcttctggt gcgcgatctc cggcgacggc aggttcgtcg
     2401 gcttcacgtc gcgcgcctcc aacctcatcg gcgcggaccg caacgacgcg accgacgtct
     2461 tcctgttcaa ccgtgagacc gagacgccgg agttcgtgag cgtccgctcg accgggacgc
     2521 aggggaaccg cgggtccgac agcccgtcgc tctcgtacac gggccgctac gtcgccttcg
     2581 cgacggcggc ttcgaacctc gcgatcaagg gcgaccgtaa cggcgtcaac gacgtgttcg
     2641 tctacgaccg caacgagggc cgtacgaacc gcgcgagcat ccgctcggac ctcggcgagg
     2701 ccaacgggtc gagctacgac ccgtcgatct cgtcgaacgg ccgcttcgtc gccttcacct
     2761 ccagcgccga caacctcgcg gtcaaaggcg acaacaacgg gcgcgaggac gtgttcgtgc
     2821 acgaccgcac cacgctgagg accaaccgcg tgagcatcag ctcgaagctc aaggagggca
     2881 acggttccag ctactcgcct tcgatctccg gcgacggcag gatggtcacg ttcgtctcgc
     2941 aggcgaacaa cctcgtgcgc cgggacacga accgcaggcc ggacatcttc gtctacaacc
     3001 gctcgacccg gaagacgatc agggtgagca tcgccggcga cggcacccag gcgaacggcg
     3061 agagctcggc ccccatgatc tcggcggacg gcggcttcgt cgccttccac tcgaacgcct
     3121 cgaacctcat cgccagcgac acgaacaacc gcaccgacgt gttcgtgcac gacctctcga
     3181 gcggcgagac caccaggatc agccgaggca tggcgaactc cgagcccaac ggcgacagct
     3241 tcatcccggc gatctcggcc gacggccgct acgtcgcctt ccagtcgacg gcgtcgaacc
     3301 tcgtggccgg cgacaccaac ggcaccccgg accagtacca gggcctcgac gtcttccgct
     3361 acgaccgtca gacgaaccag accgagcggg tcagcgtcgg tgacgacggc gagcaggcca
     3421 acgggccgag caacgacccg ggcgacgacc cgtcgatcac cgccgacggc cgctacgtct
     3481 gcttcgcatc gttcgccagc aacctggtcg aggacgacac gaacggcgcc tacgacgtct
     3541 tcgtgagggg acctctgctc taggcggacc tcgaccggcg tgggggccgg gccgcaagca
     3601 agccagtcac gcggcccggc ctcttcgcgc ttcgcacacg aacacaccgc gacgcctcgg
     3661 cgtcgcgcca aacgaaagga gagaactaag gtggccctgc aatcagcact gaggaggctt
     3721 cgagacatgt gcccaccgtt cgcgatgccc ctcgtcgggt tcctcgcagt ggcgccgctg
     3781 tgcctgagcc tctcggtccg gcccgctccc gccgaggcgg cttacgtcgg caacaacgga
     3841 aggatcctgt tcgaggcacg ccgggacggc aaccccgaga tctacgtcat ggacgccgac
     3901 ggcacgaacc aggagcgcct gacgttcagc aagacgtggg acatcgccgc gacgtggtcg
     3961 ccggacggca cccggatcgc gtggggcagc gaggaggacg gcgaccgcga gatcttcgtg
     4021 atgaacgcgg acggctccga ccagacgcag ctcacgttca accgctacta cgaccgggcc
     4081 cccacgtggt cgcccgacgg gaccaagctc gctttcgcga gcgagcgcaa caggcagtac
     4141 gcgatctacg tgatcaacgc cgacgggacg ggcggcgaga cgcgcatcac gccgccgggc
     4201 atgagcgccc gtcaccccgc gtggtcaccg gacggcacga agctcgcgtt cgagagccgc
     4261 cgcgacggca acccggagat ctacacgagc cggctgaacg gcaagggcct gcgtcgcctc
     4321 acgcacgacc cggcgaacga cttcgtgccg aactggtcgt cggacggcga gcacctcgtc
     4381 ttcgccagca cccgcgacgg caaccacgag atctactcgg tggccgccga cggcagcgac
     4441 gagcggcgcc tcaccgagaa cgtgttctac gactccgagc ccaactggtc gccggacggc
     4501 tcgaaggtcg tcttctggag cacgcgccac ggcaacatcg acatctactc gatcaacccc
     4561 gacggctcgg gtgagacgag gctcaccgac catcccggca aggactacag cccggactgg
     4621 cagccgctgt gcaccgtcgc cggcacttcg ggcgacgatc tcctcaccgg gaccctcggc
     4681 gacgacctca tctgcggggg cggcggcgac gacacgatca tgggcggagg cggcgccgac
     4741 gtcatcttcg ggggcgacgg cgacgactcg atctccgggg ggccgggcaa cgacgtcatc
     4801 gccggaggcg agggcgcgga cgacctcctc ggcgaggacg ggctcgaccg gatcttcggg
     4861 acggcgggcc cggacacggc cgacgggggg ccgggtgcgg acgtggtcaa cgtcaaggac
     4921 ggcgacgggg acgacgacct ggacggcggc ggggggtcgg acaagtgcgt gagcgacagc
     4981 ggagacggca agaccaactg cccgtgagcg caagcagaat gaggcggcgg ctctgggcgc
     5041 tcgtcttcct ccagcttctg gtcgccaccg cggccctcgc gaacgagaac gagaccgcca
     5101 actgggtccc gttcaccggc agctactcga tctcccgcac gttccagcac tacggcggcc
     5161 acgacacgcc cgggatcgac ttccgcatcc ccgccgggac gccgatccgg gcggcgggcc
     5221 ccgggaccgt cgtcgggatc gaggagcgct gcaagcccgg ccaccgctgg tgctcgggcc
     5281 ggcgcgggaa ctacgtgcag atagcgcacc ccgccggacg gcactcccgc tacctgcacc
     5341 tgaggccgcg cggagtcgtg gtctcgctcg gcgagcaggt cgtccgcggc caggtcatcg
     5401 ggtactcggg gaacaccggg gactcgaccg ggccgcacct ccactacgac gagctcctca
     5461 acgggtcgcg catctacccc ggcccgatgt acgcgctgca cggcggagag acggtggcgt
     5521 accgggactg gcccggcgcg gacgagcgct tgatccgcaa cgacggctac ccgctgacgg
     5581 acctgacggt cgcgatcgag ggggccggga gggtcacgtc ctcgccggcc gggatcgatt
     5641 gcggccccga ctgcgtagag acctacgagc cgggcgagct ggtggtcctg accgccgagc
     5701 cggacgagct gtacacgttc gcgggctgga gcggcgcctg cgaaggaacc gacccgtgcg
     5761 agctgcggat gacctcctcg atgaccgtga cggcgacgtt cgtgctgctc ccgtagggcc
     5821 ggtgcgccac cgagttctgc gcggggtcct ggtggtgtgc tcgatctcgg tcgccggctg
     5881 cacggagtcg ccgccggacc ccacgcgcga cgagccgacc gcgcccaact cggtccacgt
     5941 gatcgtcgac gacgaggcgc gacaggccgc cgtaggggtc ggggtcgacc tggacgcagc
     6001 ggtggcggcc gcgctgcgca aggtggcgaa ggagatcgac ctcccgatga cgacggtctc
     6061 ggtcgccgtc cgaccccgcg aagtcatccc cgaggtcggc atcggcgggt acacggacca
     6121 cctgggcgac gtccacgtgt tcctggaccc gcggcgggac gatctcgcgg atgtcctgcg
     6181 cgtctggctc gagccgatgc ttgcccacga ggcccatcac gctgcgcgga ccgtcgacgg
     6241 tcccggctac ggccgctcgc tcgtagaggc gatcgtcagc gaaggactcg ccgacgccta
     6301 cagcgaggag gcgttcccgg aaacgccggc gctcccctgg acgcaggctc tcgaaccggg
     6361 cgctatctgt ccgtggtgga ggcgcgcctc gggcgagcgc ggcgcctacg accacgagga
     6421 gtggttcttc ggcgcagggc gggcgccgcg gtgggccggc tacaccctcg gctacgcgct
     6481 cgtccggcgg tacttgcggg cccacgcgac ctcggctgcg gactccgtcc gaacccctgc
     6541 ccgagtgatc gtgcgaagcg ccgacctctg caggtcctaa ggccccgacg gccttctcgc
     6601 cgcaaccggt ccggccggtc ccgaggggcg cggcccggcc ctgttcgcgg gccctgcggg
     6661 gcttcacgaa cgaacgcgca agggccgggg ccgccggtgc tgcgtgttag ttttccgccg
     6721 tacctaccag gacatttgca tttttcgccg cgtccggaaa cgagcactag aggagaggct
     6781 cagccttgcc cgacggctac ttcggtcagt acgtcaccat cgggatct
//
LOCUS       DASQDO010000569         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_976900, whole genome shotgun sequence.
ACCESSION   DASQDO010000569 DASQDO010000000
VERSION     DASQDO010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976900"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..828)
                     /locus_tag="VEV43_15575"
     CDS             complement(139..828)
                     /locus_tag="VEV43_15575"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014479231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HYP24987.1"
                     /translation="MPAAPTIVPIGGAGFSPAILQFVLDLCERPRPRVCFVPTASGDS
                     SDYVVRFYEAFRDLDSVPSHVALHGTPEREAVRRQLLDQDAIYVGGGNTANMLAVWRL
                     HGADVVLREAWEQGIVLAGVSAGANCWFEASTTDSFGPLSGLRDGLGFLPGSFSPHYD
                     AEPERRPTYQRLVSEGFPAGFAADDGVALHFTWTELVETVAAGAGSAFRVEPGPGGTA
                     RETALDLRRLG"
     gene            complement(833..1207)
                     /locus_tag="VEV43_15580"
     CDS             complement(833..1207)
                     /locus_tag="VEV43_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP24988.1"
                     /translation="MSQKILVIEDDQDIRQLFDLELRAVGFDVAFARDAVSALGIVRK
                     EMPDLIVLDIGLPGGDGFLVMERLNAFDALERIPVIVVSARTAPDAHARALALGAEAF
                     FDKPFDADEVIGQIQRTLAQPV"
     gene            complement(1236..>1623)
                     /locus_tag="VEV43_15585"
     CDS             complement(1236..>1623)
                     /locus_tag="VEV43_15585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYP24989.1"
                     /translation="KVSHGLPVVADGGRMVQLLDNLLSNAVKFSRDGGTVHVRAWSAN
                     GEAVIEVADSGIGIPAKEQDRLFERFFRASTATEREIQGTGLGLSVAKTIVELHGGRI
                     SCASREGEGTTFRVALPLAEPQAAVA"
BASE COUNT          264 a          558 c          575 g          226 t
ORIGIN      
        1 cgcgtcccgc ccgccgagac ggggaagagg tcgtggtcga tcacgacgaa cgcctccatc
       61 cccacgttcg ggtcggagag ctcgatgacg cgatggggcg gcgcgtcggg gcggtcgaga
      121 acggacatcg agcggacgct accccaagcg ccgcaggtcg agcgccgtct ctcgagctgt
      181 gccgccaggc ccgggctcga cgcggaaggc gctgcccgct cccgccgcca cggtctccac
      241 gagctcggtc cacgtgaagt ggagagcgac cccgtcgtcg gccgcgaatc ctgcgggaaa
      301 gccctcggag acgagccgct gataggtcgg gcggcgctcg ggctccgcgt cgtagtgggg
      361 cgagaagctg ccgggcagga agccgagccc gtcgcggagg ccggagagcg gcccgaacga
      421 gtcggtcgtg ctcgcctcga accagcagtt cgcgcccgcg ctcacgccgg caagaacgat
      481 cccctgctcc caggcctctc ggaggacaac gtcggcgccg tgcaagcgcc agacggccag
      541 catgttcgcg gtgttgcctc cgccgacgta gatcgcatcc tggtcgagga gctgccgccg
      601 caccgcctcg cgctcgggcg tcccgtgcag agccacgtgc gagggaacgg agtcgaggtc
      661 gcggaaggcc tcgtagaagc gcacgacgta gtcgctcgag tcgccgctcg ccgtgggaac
      721 gaagcacacg cgcggacgcg ggcgctcgca gagatcgagg acgaactgga ggatcgcggg
      781 cgagaagcct gccccgccga tcgggacgat cgtcggcgcc gccggcaacc gatcagactg
      841 gttgcgcgag cgtccgctgg atctggccga tgacctcgtc ggcgtcgaac ggcttgtcga
      901 agaacgcctc ggcacccagg gcgagggcac gcgcgtgcgc atccggagcc gtccgggcgc
      961 tgacgacgat caccgggatt cgctccaggg cgtcgaacgc gttcagccgc tccatgacga
     1021 ggaagccgtc gccgcccgga agtccgatgt cgaggacgat caggtccggc atctccttcc
     1081 gcacgatccc gagggcggag actgcatcgc gcgcgaacgc gacgtcgaaa ccgacggccc
     1141 gcagctcgag atcgaagagc tggcggatgt cctgatcgtc ttcgatgacg aggatcttct
     1201 ggctcatggc gacattgtgc cgatagtgat cacgttcagg cgaccgccgc ctgcggctct
     1261 gcgagcggga gcgcgacgcg gaacgtcgtc ccctcaccct ccctgcttgc gcaggagatg
     1321 cgcccgccgt ggagctcgac gatcgtcttc gccacggaga ggcccaggcc tgtgccctgg
     1381 atctcgcgct cggtggccgt cgaggcccgg aagaagcgct cgaagagtcg gtcctgctcc
     1441 ttggcgggga tgccgattcc ggagtcggcg acctcgatca ccgcctcccc gttggcactc
     1501 caggcgcgga cgtgaaccgt gccgccgtcg cggctgaact tgaccgcatt cgagaggaga
     1561 ttgtcgagga gctgcaccat gcgcccgccg tcggccacga ccgggagccc gtgactcacc
     1621 ttc
//
LOCUS       DASQDO010000570         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_915698, whole genome shotgun sequence.
ACCESSION   DASQDO010000570 DASQDO010000000
VERSION     DASQDO010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915698"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="pilO"
                     /locus_tag="VEV43_15590"
     CDS             <1..674
                     /gene="pilO"
                     /locus_tag="VEV43_15590"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HYP24990.1"
                     /translation="GRVNARTRLILTVVASVVVILLAYFLLIRPRQGELSDVQAQVET
                     EENLTLELRGQLEALQQLQRNAPQLQADLDRIRDLVPQDHEVPNFIFQVNAAAAASGV
                     EIVQLTPELPKQPPEGAQLAQVRITIGGEGGYFAIQDFVRRLYSLDRAVRIDVLDLSA
                     LAEETGGTTGGITMTAAARIFFEAPTGVTGIAPTTPATPVTPTAPPATPAPDPAASPT
                     AAATP"
     gene            684..1361
                     /locus_tag="VEV43_15595"
     CDS             684..1361
                     /locus_tag="VEV43_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24991.1"
                     /translation="MATPPEIQETRNVNTTQIALAAGGVLLLLVVLWFAFLRGGGEDA
                     VETAPAPPPVDTETEPIPPVTPGDNDPGDKKGPVETFEVFAPKDPFKPLIVAGTGTDP
                     GVPPPTGVDADGDGQIDPGTGTTDPGTGTGTDPGTGGTGDPGTGSGTGGGGENVGGHR
                     VSLVDVFRAQGGQRAQVQVDGTVYTVEEGDRFAESFQLLSISGECATMLYGDDQFTLC
                     EGEEILK"
     gene            1392..2549
                     /gene="aroC"
                     /locus_tag="VEV43_15600"
     CDS             1392..2549
                     /gene="aroC"
                     /locus_tag="VEV43_15600"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYP24992.1"
                     /translation="MARLRFLTAGESHGQALVGILEGLPAGIPITTDEVAAELTRRRH
                     GYGRGPRMKIEKDACEIVAGVRGGRTLGSPVGVVIRNSEWEKWAHVMPVEGEPGGREL
                     TRPRPGHADLPGMLKYGFSDARNVLERASARETAARVALGTFAKALLRSVDVDVVSHV
                     VSIGPARVEGDVSVSAADLERIDASPVRCADPAAEAAMVAEIEAAGAEGDSLGGVVEV
                     VAFGLPPGLGSHVHWDRKLDGRLGGALMAVPAIKGVEIGDGFEVAARRGSASHDEIEP
                     DLSRATDRAGGTEGGMTIGGTLRARAAMKPLSTLKKRLRSVDLSTGEPVEAFQERTDV
                     CAVPAAGVVCEHVVALVLADAFLEMFGGDTLEDLEAAVARYRERVAARSPR"
     gene            2546..4126
                     /gene="aroB"
                     /locus_tag="VEV43_15605"
     CDS             2546..4126
                     /gene="aroB"
                     /locus_tag="VEV43_15605"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HYP24993.1"
                     /translation="MIVLVGFMGAGKTTVGRALAERLRLSFVDVDAAVEARAGTSIPE
                     LFARDGEAAFRRLESEVTQELLRGPDGVVALGGGALNDPAITSALEWATVVHLDVSFG
                     ESMRRVGRSTNRPMLQREDPRALFDARRASYERAADLTVAVDRRSPREIVDEIVARAA
                     VAPAGGPVRVSVALGERSYDVIVGEGVADRLAELAPPPADAEKAFVVTHPELEPAASG
                     AVASLSAAGLKTHTLTVPAGERSKSLAHATDLLSQLADLGAHRRDLVVGFGGGVVTDL
                     AGFVASTYHRGMAVVHVATTLLAQVDAAIGGKTAVNLAQGKNLVGTFHQPRAVVCDVA
                     TLRTLPPAELAAGLAEVVKYGVIADPGLLEVVEKWSEDVFGFEPQVLTDVVGRSAAIK
                     AAVVARDETEGGPRAHLNYGHTFGHAMEHVTAAGAAPLRHGEAVALGMMAAAYTAHEL
                     GRIDEAAVAAHRSALEAARLPVAARFDLGELEEAWRHDKKYRRGVRFVLLAGLGRPEA
                     GVEVDRATLETALRRLAG"
     gene            4123..4557
                     /gene="aroQ"
                     /locus_tag="VEV43_15610"
     CDS             4123..4557
                     /gene="aroQ"
                     /locus_tag="VEV43_15610"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HYP24994.1"
                     /translation="MKALVVHGPNLNLLGTRELSVYGSSTLEDVNAALEAEAKELGID
                     LDVHQSSHEGGIVDLLHAGIGDVDGALLNPAAYSHTSRAIADAVRAVPYPVIEVHLSN
                     IHAREPWRRRSVTAEAAVGVVAGFGLRSYTVALRALEGMVRS"
     gene            4558..>4964
                     /locus_tag="VEV43_15615"
     CDS             4558..>4964
                     /locus_tag="VEV43_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HYP24995.1"
                     /translation="MDYEGRIARLRDALAGAEVDALLVTNLTNVRYLCGFSGTNGQLL
                     VTSSGATFFTDPRYRARAGDLVQGAEIEVYDVNLHDSLKPRLGGERTGIEAATMTVAT
                     KAQLEGKLEVELTPTKNLVENLRRTKDAEEVSRL"
BASE COUNT          706 a         1727 c         1868 g          663 t
ORIGIN      
        1 gaggacgagt gaacgcgcgc acccggctca tcctgacggt cgtggcctcg gtggtcgtga
       61 tcctcctcgc gtacttcctg ctgatcaggc cgcggcaggg ggagctgtcc gacgtccagg
      121 ctcaggtgga gaccgaggag aacctgacgc tcgagctgcg cggacagctc gaggccctcc
      181 agcagctcca gcgcaacgca ccgcagctcc aggccgacct cgaccggatc cgcgacctcg
      241 tgccgcagga ccacgaggtg ccgaacttca tcttccaggt caacgccgcc gcggccgcgt
      301 cgggcgtcga gatcgtgcag ctgacgcccg agctgcccaa gcagccgccg gagggggcgc
      361 agctcgccca ggtccggatc acgatcggcg gcgagggcgg ctacttcgcg atccaggact
      421 tcgtgcgccg gctgtactcg ctcgaccgtg ccgtgcggat cgacgtgctc gacctctcgg
      481 cgctggccga ggagaccggt ggcaccaccg gcgggatcac gatgacggcc gccgcccgta
      541 tcttcttcga ggcacccacg ggcgtgaccg gcatcgcgcc gaccaccccc gcgaccccgg
      601 tgacgcccac cgcaccgccg gcgacacccg cacccgaccc ggctgcatcc ccgaccgccg
      661 cggcgacccc ataagaggac gagatggcga ccccaccgga gatccaggag accaggaacg
      721 tcaacacgac gcagatcgcg ctcgccgcgg gcggcgtgct gttgctgctc gtcgtgctgt
      781 ggttcgcctt cctgcgggga ggcggcgagg acgcggtcga gaccgcgccc gccccgcccc
      841 cggtcgacac cgagaccgag ccgatcccgc ccgtcacgcc gggcgacaac gacccgggcg
      901 acaagaaggg gccggtcgag acgttcgagg tcttcgcgcc gaaggacccg ttcaagccgc
      961 tgatagtcgc ggggacgggt accgaccccg gggtgccgcc gccgaccggc gtcgacgcgg
     1021 acggagacgg ccagatcgac cccggcaccg gtacgacgga cccggggacg ggcaccggga
     1081 ccgaccccgg cacgggtggc acgggcgacc ccggaacggg atcgggtacg ggtggcggcg
     1141 gcgagaacgt aggtggtcac cgcgtgtcgc tcgtcgacgt cttccgcgcc cagggcggtc
     1201 agcgcgccca ggtgcaggtc gacgggaccg tctacacggt ggaggaaggc gaccgcttcg
     1261 ccgagagctt ccagctgctg tcgatcagcg gcgagtgcgc cacgatgctc tacggcgacg
     1321 accagttcac gctctgcgag ggcgaggaga tcctcaagta gccgggccgt tcgccggggc
     1381 aagataggcg gatggcgcgc cttcgtttcc tcaccgccgg tgagtcgcac ggccaggccc
     1441 tcgtcggcat cctcgagggg cttcccgcgg gcatcccgat cacgaccgac gaggtcgcgg
     1501 ccgagctgac gcgtcgccgt cacggctacg ggcgcggccc ccgcatgaag atcgagaagg
     1561 acgcgtgcga gatcgtggcc ggcgtgcggg gcggacggac gctggggagc cccgtaggcg
     1621 tcgtcatccg caacagcgag tgggagaagt gggcccacgt catgcccgtg gagggcgagc
     1681 ccggggggcg cgagctcacc cggccgcggc ccggccacgc ggacctgccg gggatgctca
     1741 agtacgggtt ctcggacgcg cgcaacgtgc tcgagcgcgc gagcgcgcgc gagaccgcgg
     1801 cgcgcgtcgc gctgggcacg ttcgcgaagg cactgctgcg ctcggtcgac gtcgacgtcg
     1861 tgtcccacgt cgtctcgatc ggcccggcgc gcgtcgaagg ggacgtctcc gtctcggcgg
     1921 ccgacctcga gcggatcgac gcgtcgccgg tgaggtgtgc ggatcccgcg gcggaggcgg
     1981 cgatggtcgc cgagatcgag gccgcggggg ccgaagggga ctcgctgggc ggcgtcgtcg
     2041 aggtcgtcgc gttcgggctc ccgccgggac tcggctcgca cgtccactgg gaccgcaagc
     2101 tcgacggcag gcttggcggc gcgctgatgg cggttcccgc gatcaagggc gtcgagatcg
     2161 gcgacgggtt cgaggtggcg gcgcgacggg gatcggcgtc gcacgacgag atcgagcccg
     2221 acctgtcgcg ggcgacggac cgcgccgggg ggaccgaggg gggcatgacg atcgggggca
     2281 ccctgagggc ccgggcggcg atgaagccgc tgtcgacctt gaagaagcgc ctgcgcagcg
     2341 tcgacctgtc gaccggtgag cccgtcgagg cgttccagga acggacggac gtgtgcgcgg
     2401 tgccggccgc gggtgtcgtg tgcgagcacg tggtcgcgct cgtgctcgcc gacgcgttcc
     2461 tcgagatgtt cggcggcgac acgctcgaag acctcgaagc cgcggtcgcg cgctaccgcg
     2521 agcgcgtagc ggcgcggtcg cctcggtgat cgtcctcgtc gggttcatgg gggccggcaa
     2581 gaccacggtc ggccgcgctc tcgccgaacg cctgcggctc tcgttcgtcg acgtcgacgc
     2641 ggcggtcgag gcccgggcgg gcacttcgat ccccgagctg ttcgcccgcg acggcgaggc
     2701 cgcgttccgc cggctggaga gcgaagtgac ccaggagctg ctgcgggggc cggacggtgt
     2761 agtggctctc ggaggcggcg cgctgaacga ccccgcgatc acgtcggcgc tcgaatgggc
     2821 gacggtcgtg cacctcgacg tctcgttcgg cgagtccatg cggcgcgtcg gccgctcgac
     2881 gaaccgcccg atgctccagc gcgaggaccc gcgtgcgctg ttcgacgcgc ggcgggcctc
     2941 gtacgagcgc gccgccgacc tcaccgtcgc cgtggaccgc cgttctcccc gcgagatcgt
     3001 ggacgagatc gtcgcgcgcg ccgccgtcgc gcctgcgggc ggccccgtac gcgtctccgt
     3061 cgcgctcggc gagcggtcgt acgacgtgat cgtcggcgag ggcgtggccg accggctcgc
     3121 ggagctggcg ccgcctcccg cagacgcgga gaaggcgttc gtcgtgacgc acccggagct
     3181 cgagcccgcc gcgtccggcg cggtcgcgtc gctgtcggcg gcgggattga agacccacac
     3241 gttgaccgtc cccgcgggcg agcgttcgaa gtcgctcgcc cacgcgaccg acctcctgtc
     3301 gcagctcgcg gacctcggcg cgcaccggcg cgatctcgtc gtcgggttcg gcgggggagt
     3361 cgtcaccgac ctcgccggct tcgtcgcgtc cacctaccac cgcgggatgg cggtggtcca
     3421 cgtcgcgacc acgctgctcg cgcaggtcga cgcggcgatc ggcgggaaga cggccgtcaa
     3481 cctcgctcag ggaaagaacc tcgtcgggac gttccaccag ccgcgggcgg tcgtgtgcga
     3541 cgtggcgacg ttgcggacgc tcccgccggc ggagctcgcg gccggcctgg ccgaggtcgt
     3601 gaagtacggc gtcatcgcgg atcccggcct gctcgaggtc gtcgagaagt ggtccgaaga
     3661 cgtcttcggg ttcgagccgc aggtcctcac cgacgtcgtc ggtcgctccg cggcgatcaa
     3721 ggccgcagtc gtcgcgcgtg acgagaccga gggggggccg cgcgctcacc tcaactacgg
     3781 gcacacgttc ggccacgcga tggagcacgt caccgcggcc ggagcggcgc cgctcagaca
     3841 tggcgaggcg gtcgccctcg gtatgatggc cgccgcctac acggcccacg agctcggccg
     3901 catcgacgaa gccgccgtgg cggcccaccg gagtgcactc gaggccgctc gtctcccggt
     3961 ggcggctcgg ttcgacctcg gtgagctcga ggaagcgtgg cgtcacgaca agaagtaccg
     4021 gcgcggcgtg cgcttcgtcc tgctcgcggg actgggccgg ccggaggcgg gggtggaggt
     4081 cgaccgcgcg acgctcgaga cggcattgag gaggctggca gggtgaaggc cctcgtagtg
     4141 cacggaccga acctgaacct gctcggcacg cgcgagctgt cggtctacgg ctcgtcgacg
     4201 ctcgaggacg tcaatgccgc gctcgaggcc gaggcgaagg agctgggcat cgacttggac
     4261 gtgcaccaga gctcgcacga gggcggcatc gtcgacctcc tgcacgcggg gatcggggac
     4321 gtcgacggcg cgctgctgaa ccccgcggcg tactcgcaca cgagccgcgc catcgccgac
     4381 gcggtgcgcg cggtgcctta tccggtgatc gaggtgcacc tgtcgaacat ccacgcgcgc
     4441 gagccgtggc ggaggcgctc tgtgaccgcc gaggcggccg tgggcgtcgt cgccggcttc
     4501 gggcttcgtt cttacacggt cgcgctgcgc gcgctggaag ggatggtgag gtcctagatg
     4561 gactacgagg gacggatcgc gcggctccgg gacgcgctcg cgggggccga ggtcgacgct
     4621 ctgctcgtca cgaacctcac gaacgtgcgc tacctgtgcg gcttctcggg gacgaacggc
     4681 cagctgctcg tgacgtcgtc gggcgcgacg ttcttcaccg acccgcgcta ccgggcccgc
     4741 gccggcgacc tcgtccaggg cgcggagatc gaggtctacg acgtcaacct gcacgactcg
     4801 ctgaaaccgc ggctcggggg ggagcgcacg ggcatcgagg ctgcgaccat gaccgtcgcg
     4861 acgaaggcgc agctcgaggg caagctcgag gtcgagctca ctcctacgaa gaacctcgtc
     4921 gagaacctgc gtcgcacgaa ggacgcggag gaggtctcgc gtct
//
LOCUS       DASQDO010000571         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_304303, whole genome shotgun sequence.
ACCESSION   DASQDO010000571 DASQDO010000000
VERSION     DASQDO010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304303"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..795)
                     /locus_tag="VEV43_15620"
     CDS             complement(202..795)
                     /locus_tag="VEV43_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24996.1"
                     /translation="MATNATRGTVPAVETVGTPTLWIIGGLAIAILGGMLGSFFDPGD
                     DMQSILYSLAAVGGTVAGLLLLIRHTRARLDLSAAGFGALGVISIAGSVFSFTGPGYE
                     AVSAYLAVLHWPAMWLIAAQNWAPIWSRGAAAFAGLMFALWGYSTVLGDELPDTNSPM
                     AIVGWIGFTVAAIGWVLTLREEGEDAGTPAGATTARL"
     gene            941..1849
                     /locus_tag="VEV43_15625"
     CDS             941..1849
                     /locus_tag="VEV43_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP24997.1"
                     /translation="MTLRRTFQLVVLLVALVVGWSAIGRGSETRAAGEDEACPDTVVV
                     EEEDFDRVEATAAPGGTEGRVVEAALVAEDPEGVVMRFGKDRGPLSRKFFVALPDRSK
                     ATLPEEGSPLNVRQRPLEREEADHVIGDDDYVVEATFTGAREVTVAVCIDPAVHPGTY
                     KGQLTLEHPDIRAVVVPVEVSLQFSSWWILALLVGGTVLFAGPAYVWATRRKSAAEGG
                     HVLLSLAGLRELASWVLANFIAFGIACIAATSAFLANYWYDPSWGAEAPKDWFTLIGA
                     TFTAFTTGMLTGTAPPKGGQATRDEV"
     gene            complement(1846..2499)
                     /gene="uppS"
                     /locus_tag="VEV43_15630"
     CDS             complement(1846..2499)
                     /gene="uppS"
                     /locus_tag="VEV43_15630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HYP24998.1"
                     /translation="MSDIPRHVAIIMDGNGRWAEARGLPRTKGHEAGERALFDVVEGA
                     LEAGVEFLSVYAFSTENWARPSDEVDFLLGFNRSLLRQRRDDLNERGVRIRRIGRREP
                     VPQDVLDEFDAAEAATAGNERMELLVCFNYGGRAELEDAAAAGPIGDNLYTAGAPDVD
                     LLIRTSGERRLSNFLLWQSAYAELYFTDTLWPDFDRHALFEALSDYAGRDRRFGGLG"
     gene            complement(2496..3224)
                     /gene="recO"
                     /locus_tag="VEV43_15635"
     CDS             complement(2496..3224)
                     /gene="recO"
                     /locus_tag="VEV43_15635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HYP24999.1"
                     /translation="MPGLYKDEGVVLKTIKLGEADRIVTLFTRDHGKVRAVAKGIRKT
                     KSRFGARLEPFTHTAVLVYRGRNLDTITQADILTSFHEVRVDYARLTAAAALADVVEK
                     ITPERERAASTYRLLVQGLEALKAGAGATVVPAFLVKLLSISGYHPQLTTCAGCGETS
                     AGLGGFSPALGGAVCANCWNDDRDALNVSIDRLRLLGRLLDRDFGEPAEPDEAEELTD
                     VLRRYAEYHLERPLRSLRLLPTAP"
     gene            complement(3266..3475)
                     /locus_tag="VEV43_15640"
     CDS             complement(3266..3475)
                     /locus_tag="VEV43_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25000.1"
                     /translation="MPQATVKRYDSISRTGVLVSDDGATEWTINDAALEGSMFRFLRP
                     GQRVTFDLVEGAGGATPCNLRIGIS"
     gene            complement(3484..4224)
                     /locus_tag="VEV43_15645"
     CDS             complement(3484..4224)
                     /locus_tag="VEV43_15645"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYP25001.1"
                     /translation="MSRNRAKLGVLVRMQMARIRTAWRPYLIVSSAMPLGIAVLMRAV
                     MEPAQVEEFGEQIVAGSAVLAIAMTAIVMLAQRVAWMKESGALEYYSTVPAGRAPLIA
                     AVLLSFAVFALPGTVIVLTLGALLFDLSLAALWAAVPVWALGSFALAGLGVAIGFAAP
                     DEQLAGMYSNLAMMAALFLGILPLERIPGWLGPVRAVLPSTYAAEALKPGFAGGIAGS
                     QVWDLLILAGFGAVSFWAVSGPLWPKPE"
BASE COUNT          667 a         1552 c         1530 g          607 t
ORIGIN      
        1 cccgacccgg agccggacga cgcgacggac gggaccccgc cggaaccctg atcggcgggt
       61 tggccttcgc gacgctgggg gtctcggggg tccggtgcac gaggtgcacg aggtgcacga
      121 ggtgcacgag gtgcacgctg caccgcatgc accgcatgca ccgcagggtt cgcgaggggt
      181 gttgcgtgtg gctgctcggc cctagaggcg ggccgtcgtc gccccggccg gcgtccccgc
      241 gtcttctcct tcttcacgca gcgtcaggac ccagccgatc gccgctaccg tgaacccgat
      301 ccaacccacg atcgccatcg gcgagttcgt gtccgggagc tcgtcgccca ggaccgtgct
      361 gtaaccccac agggcgaaca tcagccccgc gaacgccgcg gccccccggg accagatcgg
      421 cgcccagttc tgggccgcga tcagccacat cgccggccag tggagcaccg cgagatacgc
      481 ggagaccgcc tcgtaacccg ggccggtgaa ggagaagacc gaacccgcga tcgatatcac
      541 accgagcgcg ccgaagcccg cagcggacag gtccagccgg gcacgggtat ggcggatcag
      601 cagcagcagc ccggctaccg tcccgccgac cgccgcgagc gagtagagga tcgactgcat
      661 gtcgtcgccg gggtcgaaga agctgcccag catcccgccg aggatcgcga tcgcgagccc
      721 cccgatgatc cacaacgtcg gggtccccac ggtctcgact gcgggcaccg ttccgcgcgt
      781 agcgttcgtg gccacacgaa tctcctctct cgaccgttcc ctcgacagag agtgcgctcg
      841 tgaacccgcc gaatcaagga ccccctaagc ctcctgcccc ctagcggcgc cggttcggtc
      901 ctgcttgact tgcggtcgag cgacgacgcg aggggaccgg atgacgctga ggcggacgtt
      961 ccagctggtg gtgctcctcg tcgcgctcgt cgtcggttgg tcggcgatcg gaagaggttc
     1021 cgagacccgg gccgccggcg aggacgaagc gtgtccggac acggtcgtcg tcgaagagga
     1081 ggacttcgac cgggtcgaag cgaccgcggc ccccggcggc acggaaggcc gcgtcgtcga
     1141 agccgcgctc gtggccgaag accccgaggg cgtggtcatg cggttcggca aggaccgagg
     1201 ccccctcagc cgcaagttct tcgtcgcgtt gccggaccgc tcgaaggcga cgctgcccga
     1261 ggaggggtct ccgctcaacg tgcgtcagcg cccgctggag cgcgaggagg cggaccacgt
     1321 catcggcgac gacgactacg tcgtcgaggc gacgttcacg ggagcgcgcg aggtgaccgt
     1381 cgccgtgtgc atcgaccccg ccgtccaccc cgggacgtac aagggccagc tgacgctcga
     1441 gcacccggac atccgcgcgg tcgtcgtgcc ggtcgaggtg tcgctgcagt tctcgagctg
     1501 gtggatcctc gcgttgctcg tgggcggcac cgtcctgttc gccgggccgg cctacgtctg
     1561 ggcgacccgg cgcaagagcg ccgcggaggg cggccacgtc ctgctcagcc tcgcggggct
     1621 tagagagctc gcgtcgtggg tcctcgccaa cttcatcgcg ttcggcatcg cgtgcatcgc
     1681 cgcgacctcg gccttcctcg cgaactactg gtacgacccg tcgtggggcg cggaggcgcc
     1741 gaaggactgg ttcacgctga tcggcgcgac cttcaccgcg ttcacgaccg gcatgctgac
     1801 gggcacggct cctcccaagg ggggccaggc cacccgcgac gaggtctagc cgagcccccc
     1861 gaacctccgg tcccggcccg cgtagtcgga cagcgcctcg aacagcgcgt gacggtcgaa
     1921 gtccggccac agcgtgtccg tgaagtacag ctccgcgtag gccgactgcc acagcaggaa
     1981 gttcgagagc ctgcgctcac ccgacgtcct gatcaggagg tccacgtcgg gcgcgccggc
     2041 cgtgtagagg ttgtccccga tcgggcccgc ggcggccgcg tcttcgagct cggcccggcc
     2101 cccgtagttg aagcacacca gcagctccat ccgctcgttc cccgcggtcg ccgcttcggc
     2161 cgcgtcgaac tcgtcgagca cgtcctgcgg cacgggctcg cggcggccga tgcggcggat
     2221 gcgcacgccg cgctcgttca ggtcgtcgcg cctctggcgc agcagcgaac ggttgaaccc
     2281 cagcaggaag tcgacctcgt cggagggccg ggcccagttc tcggtcgaga acgcgtacac
     2341 ggagaggaac tcgacgccgg cttcgagcgc gccctcgacg acgtcgaaca gcgcgcgctc
     2401 ccctgcctcg tgccctttcg tgcgcggcag gccgcgggcc tcggcccagc ggccgttgcc
     2461 gtccatgatg atcgcgacgt ggcgcgggat gtcgctcacg gagcggtggg cagcagtctc
     2521 aggctgcgca gcgggcgttc gaggtggtac tcggcgtagc ggcgcaggac gtcggtgagc
     2581 tcctctgcct cgtccggctc ggcgggctcg ccgaagtcgc ggtcgaggag ccggccgagc
     2641 aggcgcaggc ggtcgatcga cacgttgagc gcgtcgcggt cgtcgttcca gcagttcgcg
     2701 cacacggcgc cgcccagggc ggggctgaac ccgccgaggc cggccgacgt ctcgccgcag
     2761 cccgcgcacg tcgtgagctg cgggtgatag ccggagatcg acagcagctt gacgaggaac
     2821 gccgggacca cggtcgcgcc ggcgccggcc ttgagggctt cgagcccctg cacgagcagc
     2881 cggtaggtcg acgcggctcg ctcgcgctcg ggagtgatct tctcgaccac gtccgcgagc
     2941 gcggccgccg ccgtcagccg ggcgtagtcc acccgcacct cgtggaacga cgtgaggatg
     3001 tcggcctgcg tgatcgtgtc gaggttccgg ccgcggtaga cgaggacggc ggtgtgcgtg
     3061 aacggctcga ggcgcgcgcc gaagcggctc ttggtcttgc ggatgccctt cgcgaccgcc
     3121 cgcaccttgc cgtggtcgcg ggtgaacagc gtgacgatgc ggtcggcctc gcccagcttg
     3181 atggtcttga ggacgacgcc ctcgtccttg tacagccccg gcatgagaga gacagcgtaa
     3241 gtgcggcggg agagcggccg gtggattagg agataccgat gcggaggttg cacggggtgg
     3301 ccccgccggc cccctcgacc aagtcgaacg tcaccctctg gccgggccgc aggaagcgga
     3361 acatcgagcc ttcgagggct gcgtcgttga tcgtccactc ggtcgctccg tcgtcggaga
     3421 cgagcacgcc ggtccgggag atcgagtcgt agcgcttgac ggtggcctgg ggcacgtcgc
     3481 tcctcattcc ggtttgggcc acagcggccc gctgacggcc cagaaggata ccgccccgaa
     3541 tccggcgagg atcagcaggt cccacacctg cgaccccgct atgccgccgg cgaagccggg
     3601 cttgagcgcc tcggccgcgt acgtcgacgg gagcaccgcg cgcaccggtc cgagccaccc
     3661 ggggatccgc tcgagcggca ggatcccgag gaacagcgcg gccatcatcg cgaggttcga
     3721 gtacatcccc gcgagctgct cgtcgggggc cgcgaacccg atcgcgacgc cgaggccggc
     3781 gagggcgaac gagcccagcg cccagaccgg caccgccgcc cacagcgccg cgagcgagag
     3841 gtcgaacagc agcgcaccga gcgtgaggac gatcaccgtc ccgggaagcg cgaacacggc
     3901 gaacgagagc aggaccgccg ctatcagggg ggcccggccg gccggcacgg tcgagtagta
     3961 ctcgagcgcg cccgactcct tcatccacgc gacgcgctgg gccagcatga cgatcgcggt
     4021 catcgcgatc gctaggacgg ccgagccggc gacgatctgc tcgccgaact cctcgacctg
     4081 cgcgggctcc atcaccgcgc gcatcaagac ggcgatcccg agcggcatcg ccgacgacac
     4141 gatcaggtac ggacgccatg cggtcctgat ccgcgccatc tgcatgcgca cgaggacgcc
     4201 gagcttcgct cgattccggc tcacgatccc tcttcctcct ggaacgtccg gcccccgatc
     4261 tcgaggtaga cgtcctccag cgtcggcgag accagccgca cgtcctcgag ggactcgagt
     4321 cccgcggccg cgatcacctt ctcgagcgac gggatc
//
LOCUS       DASQDO010000572         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_304361, whole genome shotgun sequence.
ACCESSION   DASQDO010000572 DASQDO010000000
VERSION     DASQDO010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304361"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VEV43_15650"
     CDS             complement(<1..181)
                     /locus_tag="VEV43_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYP25002.1"
                     /translation="MSDPSKSARADEILRAETARFEERTPRSRELYDRAAASMPFGVA
                     SSFQAGDPYPIYLTGG"
     gene            234..1196
                     /locus_tag="VEV43_15655"
     CDS             234..1196
                     /locus_tag="VEV43_15655"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HYP25003.1"
                     /translation="MLMAICHSAEDGWSDVEDLTSVSDLRGTSGILLWAETDVSSLTA
                     DDVATIAEEFELHPLAVEDAIHARQRPKVEVYDTHLFVVFDQLDLVNDQLEAVQIACF
                     VGERYVLTLHAGAARSIAIAKQRWARAQQELGQGPPYLLHTLLDSVVDDYQRIADDLE
                     LRVEELEELVLESPGAPVSRQLYAVKQKVSRLRRYALPVARVLEGVKTHPGTPSDALA
                     HFGDVHDHVLRIADQVRNVDDLSQAVLDLTRAEQAYALNEVTKRLTGWAAVIAVPTFI
                     ASVYGMNFELMPESGQLFGFWFALSLMTVSAVGLYVFLKKREWI"
     gene            1284..1562
                     /locus_tag="VEV43_15660"
     CDS             1284..1562
                     /locus_tag="VEV43_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25004.1"
                     /translation="MAEGKQKKVTQRVTLDVYLTAPDAKPRKSHAGELKRLQKLGFRE
                     VDRRLGGREDQVRVTLEREVDRYPGVPLPPVPPDIVERCAEGSPPCSP"
     gene            1529..1972
                     /locus_tag="VEV43_15665"
     CDS             1529..1972
                     /locus_tag="VEV43_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYP25005.1"
                     /translation="MRRRVAALFAVALMSAAACGESAENDDPEPPPRGDEGAAAGVSL
                     RATDFAFDPELIEVGPGEDAEITFVNAGNVAHTFTAEEIDVDAEAASGEEASASFTAP
                     DEDATIEFVCRFHPSEMKGEIVVGDGGSGAGGSGGEGEDSGNVDY"
     gene            complement(1991..2644)
                     /locus_tag="VEV43_15670"
     CDS             complement(1991..2644)
                     /locus_tag="VEV43_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP25006.1"
                     /translation="MTTALLAHYVVGPLGCNCYVVGDPATKEAIVIDPGGDGALLIEE
                     LARLGVTVKAIVATHAHFDHLIAGQELRAHTGAPFHLHDADRFLLDWWHESGRLFLGI
                     DLPPPPDIDADAGEGLVLQAGETRLEVLHTPGHSPGSISLVGANALFSGDALFAGSVG
                     RTDLPGGDTKLLLDAIKSKLFAFGDETPVYPGHGEPTTIGEEKERNPFVGRNARWTP"
     gene            complement(2641..>3165)
                     /locus_tag="VEV43_15675"
     CDS             complement(2641..>3165)
                     /locus_tag="VEV43_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25007.1"
                     /translation="LVAVVAGVAGGSSRQGAGGILLALMAAPIAAAALAAVGVGGSVD
                     VVLDAGLLERPLRQSLSLWEGAGPYVLALAAVPAVAAVIGGRVAAAVWAQVSPLVRGL
                     RNGAALTIALVIASIVGSLAATSGAGGDLLSVRLGFFPGIVGFAIALAWGAAGAYAGP
                     RLPLPRRRPRVDDP"
BASE COUNT          512 a         1113 c         1113 g          427 t
ORIGIN      
        1 ggccccccgt caggtagatc ggataggggt cgccggcctg gaaggaggac gcgaccccga
       61 acggcatcga cgcggcggcg cggtcgtaga gctcgcgcga acgcggggtc ctctcttcga
      121 agcgcgcggt ctcggcgcgc aggatctcgt ccgcccgcgc gctcttcgac ggatcgctca
      181 tgagaagacc ttagccgccg cccgcgcacc tgggttggcc gtatcgtcta aagatgctca
      241 tggccatctg ccactcggca gaagacgggt ggagcgacgt cgaggacctc accagcgtgt
      301 cggacctgcg cggcacgagc gggatcctcc tgtgggcgga gaccgacgtg tcgagcctca
      361 ccgccgacga cgtcgcgacg atcgccgagg agttcgagct ccacccgctc gcggtcgagg
      421 acgcgatcca tgcccgccag cgcccgaagg tcgaggtcta cgacacccac ctcttcgtcg
      481 tcttcgacca gctcgacctc gtcaacgacc agctcgaggc agtgcagatc gcgtgcttcg
      541 tcggcgagcg ctacgtcctc acgctgcacg cgggcgcggc ccgcagcatc gccatcgcga
      601 agcagcgctg ggcgcgcgca cagcaggagc tcggccaggg tcccccgtac ctgttgcaca
      661 cgctgctcga ctccgtcgtg gacgactacc agcggatcgc cgacgacctc gagctacgcg
      721 tcgaggagct cgaagagctc gtgctcgaat ctcccggcgc cccggtgtcg cgccagctct
      781 acgcggtcaa gcagaaggtc tcgcggctgc gacggtacgc gctccccgtg gcgcgcgtgc
      841 tggagggcgt gaagacacat ccggggacgc cgtcggacgc gctggcgcac ttcggcgacg
      901 tgcacgacca cgtgctccgg atcgccgacc aggtgcgcaa cgtcgacgac ctgagccagg
      961 ctgtgctcga cctcacgagg gccgagcagg cgtacgcctt gaacgaggtg acgaagaggc
     1021 tgaccggctg ggccgccgtc atcgcggtcc cgacgttcat cgcgagcgtg tacgggatga
     1081 acttcgagct gatgccggag tcgggtcagc tgttcgggtt ctggttcgcc ttgtcgctga
     1141 tgaccgtcag cgccgtcggg ctctacgtgt tcctcaagaa gcgggagtgg atctagaaaa
     1201 ggacgtttgg accagtcagg atcccggcgg aaccttccca cctcctcgtc cgaacgtctt
     1261 tttgccctgg taacctcggg cgcatggcag aaggcaagca gaagaaagtc acgcagaggg
     1321 tcacgctcga cgtctacctg accgcacccg acgcgaagcc gcgcaagagc cacgcaggcg
     1381 agctgaagcg cctgcagaag ctcggcttcc gcgaggtcga ccggcgtctc gggggccgcg
     1441 aggaccaggt ccgcgtcacg ctcgagcgcg aggtcgaccg ctaccccggc gtcccgctcc
     1501 cgcccgtccc tccggacatc gtcgagcgat gcgcagaagg gtcgccgccc tgttcgccgt
     1561 agccctgatg agcgcggcgg cgtgcggcga gtccgcggag aacgacgacc ccgagccgcc
     1621 tccgcgcggt gacgaggggg cggccgcggg agtgtcgctg cgcgcgaccg acttcgcttt
     1681 cgaccccgag ctgatcgagg tcggccccgg cgaggacgcc gagatcacgt tcgtgaacgc
     1741 cgggaacgtc gcccacacct tcaccgccga ggagatcgac gtcgacgccg aggccgcttc
     1801 gggcgaggaa gcctcggcgt ccttcacggc ccccgacgag gacgcgacga tcgagttcgt
     1861 ctgccgcttc catccgtcgg agatgaaggg cgagatcgtc gtaggtgacg gcggctcggg
     1921 ggccggtggc tccggcggcg agggcgaaga ctccggcaac gtcgactact gatccgctcg
     1981 ccgggcccgc ctaaggcgtc cagcgcgcgt tgcgtcccac gaaggggttg cgctccttct
     2041 cctcgccgat tgtcgtcggc tcgccgtgac cggggtagac cggcgtctcg tcaccgaacg
     2101 cgaacagctt cgacttgatc gcgtcgagca ggagcttcgt gtcgccgccc ggcaggtccg
     2161 tcctcccgac cgatcccgcg aacagagcat caccggagaa cagcgcgttc gcaccgacga
     2221 gcgatatcga gcccggtgag tgccccgggg tgtggaggac ttccagtcgc gtctcgcccg
     2281 cctgcagcac gagaccctcg cctgcgtcgg cgtcgatgtc gggcgggggc gggagatcga
     2341 tcccgaggaa cagccgtccc gactcgtgcc accagtcgag caggaaccgg tcggcgtcgt
     2401 ggaggtggaa cggcgctccc gtgtgggctc gaagctcctg ccccgcgatc aagtggtcga
     2461 agtgcgcgtg cgtcgcgacg atcgccttca cggtgacgcc gagccgggcg agctcctcga
     2521 tcagcagggc cccgtcgccg cccgggtcga tgacgatcgc ctccttggtg gcgggatcgc
     2581 cgacgacgta gcaattgcac ccgagcggtc cgacgacgta gtgcgcaagg agggccgtgg
     2641 tcatgggtcg tccaccctag gacgacggcg cggcagcggg agccgggggc ccgcgtaggc
     2701 cccggccgcg ccccatgcaa gggcgatggc gaacccgacg atcccgggga agaagccgag
     2761 ccgcaccgag agcaggtcac ccccggcacc gctagtggcc gcgagcgacc cgacgatcga
     2821 ggcgatgacg agggcgatgg tcagcgcggc cccgttccgc agcccacgga cgagcggcga
     2881 gacctgggcc catacggcgg cggccacccg gcccccgatc accgcggcga cggcgggcac
     2941 ggcggcgagc gcgaggacgt agggcccggc cccctcccac agcgacaacg actggcgcaa
     3001 gggccgctcg agcaagccgg cgtcgagcac gacgtcgacg gaccctccga ccccgacggc
     3061 ggcgagcgcc gccgccgcga tgggcgcggc catgagagcg agcaagatcc cgccggcgcc
     3121 ctgccgggac gagcccccgg cgaccccggc gaccacggcg acgag
//
LOCUS       DASQDO010000573         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1099894, whole genome shotgun sequence.
ACCESSION   DASQDO010000573 DASQDO010000000
VERSION     DASQDO010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099894"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEV43_15680"
     CDS             <1..448
                     /locus_tag="VEV43_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25008.1"
                     /translation="AWSWGAGDTGSNHQAPPETEFEDVCQVAPKAAPPPEKARPKRAG
                     TGEKKGAQPPAPFAAEPEPGDAERPADPRKRRPQSRPRVREGHGRHVDPAAPQPSPLP
                     SEPVSVAAEPASDESGDGPPVTGLVGLAAAGALGAVAWRGSRRKKV"
     gene            452..1249
                     /locus_tag="VEV43_15685"
     CDS             452..1249
                     /locus_tag="VEV43_15685"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HYP25009.1"
                     /translation="MHPGAWFAWVAGAGVVAIATTNPFYLAPLFVAAWAVHSRKGVDG
                     PAARSFRTFALFAAVAVVARTVLVVFSPPLSWNGVAYAALEGLRLGTLLVLFGTFNSV
                     TDPFRVLRLAPRRWHEPALAAALALSIAPRTMEAAAKVRESQRLRGIEIKSWRTLPAL
                     AVPVLATGMEDAVTLAESMDARGHGSGRRTRYRPDRFGRSAGLTVALAVAGAAAVVAL
                     AVRGEGALTVSTYPLRWPGAEVLPLLASFAFAAPAFLPADPVERKVA"
     gene            1246..1521
                     /locus_tag="VEV43_15690"
     CDS             1246..1521
                     /locus_tag="VEV43_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYP25010.1"
                     /translation="MSAAIVFDSVSFSFAEDAPRVLDRVSLAIEPGTFVLVTGATGAG
                     KSTFLRACNGLVPHFSGGTFEGEVTVGGLGTVRHAPRELAGTVAFVP"
BASE COUNT          197 a          584 c          623 g          233 t
ORIGIN      
        1 cgcgtggtcg tggggcgccg gcgacaccgg gtcgaaccat caggcaccgc cggagaccga
       61 gttcgaagac gtgtgccagg tcgcgccgaa ggccgcgcct ccgccggaga aggcgcggcc
      121 gaagcgggcc gggaccggcg agaaaaaagg cgcgcaaccc ccggccccct ttgcggccga
      181 acccgagccc ggggacgcgg aacggcctgc cgatccgcgg aagagacggc cacagagccg
      241 gccccgggtg cgggaggggc atggccggca cgtggacccc gcggcaccgc aaccgtcacc
      301 cctcccctcc gagcccgtct cggtcgccgc cgagcccgcg tcggacgagt ccggtgacgg
      361 cccgccggtg acggggctcg tcgggctcgc agcggccggc gcgctcggtg cggtcgcgtg
      421 gcggggctcg cgccggaaga aggtctgagc agtgcacccc ggcgcctggt tcgcgtgggt
      481 cgcgggggca ggggtcgtcg cgatcgcgac gacgaacccc ttctacctcg cgccgctgtt
      541 cgtcgccgcg tgggccgtgc actcgcgcaa gggcgtcgac ggtcccgcgg caaggtcgtt
      601 ccgcacgttc gcgctgttcg cggccgtcgc ggtcgtcgca cggacggtgc tcgtcgtgtt
      661 cagcccgccg ctgtcgtgga acggcgtcgc ctacgcggcc ctcgagggcc ttcgactcgg
      721 cacgctgctg gtgctcttcg gcacgttcaa ctccgtgacg gacccgttcc gcgtcctgag
      781 gctcgcaccg cggcggtggc acgagcccgc gctcgccgcg gcacttgctt tgtcgatcgc
      841 accgcgcacg atggaggccg ccgcgaaggt gcgcgagtcg cagaggctgc gggggatcga
      901 gatcaagagc tggcggacgc tgcccgcgct cgcggtgccc gtgctcgcga ccggtatgga
      961 ggacgccgtg accctggcgg agagcatgga cgcgcggggg cacggcagcg ggagacgcac
     1021 ccgctaccgc cccgaccgtt tcgggcggtc cgccgggctc acggtcgcgc tcgcggtcgc
     1081 cggagcggcg gccgtcgtcg cgctggcggt gcgcggcgag ggcgcgctga cggtctcgac
     1141 gtatccgctc cggtggccgg gggccgaggt gctgccgctg ctcgcgtcct tcgcgttcgc
     1201 ggcccccgcg ttccttcccg ccgacccggt cgagaggaag gtcgcgtgag cgccgcgatc
     1261 gtcttcgact cggtctcgtt ctcgttcgcc gaggacgccc cccgcgtcct cgaccgagtg
     1321 tcgctcgcga tcgagcccgg gacgttcgtg ctcgtgacgg gagcgaccgg cgcggggaag
     1381 tccacgttcc tgcgcgcgtg caacggtctc gtcccccatt tcagcggagg gacgttcgaa
     1441 ggcgaggtca ccgtcggtgg gctcggcacc gtgcgccacg caccgcgcga gctggccggc
     1501 accgtggcgt tcgtcccgta ggacggctcg atcgcgtttt acgtcgaccg ggtcgaggac
     1561 gtgcactcct acggtatgga gaacctaggg atagcgcccc cggttatgag gcgccgcgtc
     1621 gaggaggtgc tggacct
//
LOCUS       DASQDO010000574         5363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_120913, whole genome shotgun sequence.
ACCESSION   DASQDO010000574 DASQDO010000000
VERSION     DASQDO010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120913"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VEV43_15695"
     CDS             complement(<1..1041)
                     /locus_tag="VEV43_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HYP25011.1"
                     /translation="MTIPRTAHARSGKALLAIALGVVASLALTTFALVPGATNASSHR
                     EAPLTAADPQVDNTDVYAFVSPDNPATMTLVSNWIPFEEPAGGPNFYSFAEGVRYDVN
                     ISNDGDANPEIVYRWRFRDHYRNPNTFLYNTGQVTTLDDEDLNFYQTYTLRKIRVGKG
                     GKTLVRNAPVAPSDVGAASMPDYEDLRDEAIVTAGAGVSAFAGQTNDPFFLDLRVFDL
                     LYGADFSEVGDDTLRGFNTNTMVLQVPKTEVAKGADVVSNPVVGIWSTASRRSTRIQT
                     AKGTQSFRGPWVQLSRLGMPLVNEVVVPVGAKDYFNASKPKGDQQFLGAVDDPELPRL
                     IEAVYGIPAPDSN"
     gene            complement(1127..1624)
                     /locus_tag="VEV43_15700"
     CDS             complement(1127..1624)
                     /locus_tag="VEV43_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016202820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYP25012.1"
                     /translation="MGVRNYLIEGLSGTGKTTVCNELQRRGYHAVHGDRELRAHPRTD
                     GVTENRLPPIWDVARVNALIANHDEVMTFLCGGCRNSSQFIELLDGVFVLEVDDVDTV
                     MRRIDERVLVDPSDWGGRQEEREIIMRLHHTKETVPQNGISIDATAPITQVVDSILRH
                     CEAGT"
     gene            complement(1736..3304)
                     /locus_tag="VEV43_15705"
     CDS             complement(1736..3304)
                     /locus_tag="VEV43_15705"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HYP25013.1"
                     /translation="MKHTKTLAVVVGAAALIGTGSAAATPNGGWARDTRAGYTPFEDF
                     QPVPASAPCPGEPTGYEAKPFVLPGGYEQQVILEELVPPPAGSGTGTVPYDLWDMNTQ
                     NETGTDAGRYVYRTHETGNNGAVTFLDLQTGETGVIAERADFERLDGLAWTPWNTLLA
                     AEEVIVAAHKDPTVPQAQGGLVYELFLDPSDPTRLAPWREPITAPQDGTQDYAKDGIR
                     ARPAVGARSHEGLRFDRKLNLYGIAESRGVQASRSGGIFRFEPDDKQNPLATGQLYAL
                     QTQNRHDGEGRWIPLDREASQVDSDTHAEQRGANEYERPEDVETDTSTGRDVNGNGDT
                     LYVAITGTDEVLAIDLHNQNRPFAYHYVYDASKPHTNTTGDPNVAADFDAPDNLALDA
                     EGNLAITEDPGSDVAGGKKAGDDIWIAAPPQGPGNPNAPGQQGRRREEARTVQRFASL
                     KDCDAEPSGVYFLMEGTQDYVEQWDPALADYVHDETLLVHRMHSGQFGPEDQLVAITP
                     DEIDEDDEKSKRNK"
     gene            3622..4221
                     /locus_tag="VEV43_15710"
     CDS             3622..4221
                     /locus_tag="VEV43_15710"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HYP25014.1"
                     /translation="MVAGVCRQLAEEFTGIFTPETVKRFVAESFEALSGARVQAFVPL
                     FIHRFAKERLEAVAQAEGTILKEVPEVLFVCVHNAGRSQMAAALLDRAGQGRVHVRSA
                     GSTPANEIHAGVREAMAEIGIDMTGEFPKPLTDEVVRAADVVITMGCGDACPVYPGKR
                     YLDWELPDPANKPIDEVRPIREEIDRRVRELLAELVPAG"
     gene            complement(4256..>5363)
                     /locus_tag="VEV43_15715"
     CDS             complement(4256..>5363)
                     /locus_tag="VEV43_15715"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HYP25015.1"
                     /translation="SGAITSLLLDRDFNVLESSYNAGGMVSNCSGSGTPWGTVLTGEE
                     STATLDADHGFIWEVDPHDHTKVRLDACGRFDHETAVVDAETGFVYLTEDAGEGLLYR
                     MRPRKRRDLSKGILEAFAGKGRWVRIRDPLGVEGESPATQGARAGAVVFQRLEGGSFD
                     GRYFYFTETQDETACGKVWRLHVDRGRLELFAEGDAGGGMCMPDNLVFDAARNMYVTE
                     DKPTPDPSDPNRIQFIDRATGDVSVFGEVTTPGDEATGPAFVPGGRAMFLNLQRSGNG
                     GLTLVIEGPFPRRRSRQRAVAAMPPQARPEESRFLSEHGADLPSLATLPLAAAAAAVN
                     LRRRGRFVEGLPRDLDQAAQELGEPGFKANPKRT"
BASE COUNT          781 a         1764 c         1821 g          997 t
ORIGIN      
        1 gttcgagtcc ggcgcgggga tcccgtagac ggcctcgatc aggcggggca gctcgggatc
       61 gtcgacggcg ccgaggaact gctggtcgcc cttcggcttc gaggcgttga agtagtcctt
      121 cgccccgacg gggacgacga cctcgttgac cagcggcatc cccagcctcg acaactggac
      181 ccacgggccg cggaagctct gcgttccctt cgccgtctgg atccgggtgc tgcgacggga
      241 cgcggtcgac cagatcccga ccacggggtt cgacaccacg tcggcgccct tggcgacctc
      301 ggtcttggga acctggagca ccatcgtgtt ggtgttgaag ccgcggagcg tgtcgtcccc
      361 cacctcgctg aagtccgctc cgtagagaag gtcgaacacg cggaggtcga ggaagaacgg
      421 gtcgttcgtc tggccggcga acgcgctgac gcctgcgcct gcggtcacga tcgcttcgtc
      481 acgcaggtcc tcgtagtcgg gcatcgacgc cgcgcccacg tcgctcggcg cgacaggcgc
      541 gttgcgcacg agggtcttgc cgcccttgcc cacgcggatc ttcctgagcg tgtacgtctg
      601 gtagaagttc agatcctcgt cgtccagcgt cgtgacctgg ccggtgttgt agaggaacgt
      661 gttcggattg cggtagtggt cacggaacct ccaccggtac acgatctcgg ggttcgcgtc
      721 tccgtcgttc gagatgttga cgtcgtagcg gacgccctcc gcgaaggaat agaagttcgg
      781 cccgcccgcc ggctcctcga acgggatcca gttgctgacg agcgtcatcg tcgcgggatt
      841 gtcggggctg acgaacgcgt agacgtcggt gttgtcgacc tgagggtcgg ctgcggtgag
      901 gggcgcctcg cggtggctcg acgcgttggt cgcaccgggc acgagcgcga aggtggtgag
      961 cgccagggac gcgaccactc ccagagcgat ggcgaggaga gcctttccac tccgcgcgtg
     1021 ggcagttcga gggatcgtca tggttctcct tcgttcgggg cggctgtcgc gtctagttcg
     1081 acggcagtcg ggttacggat tggttacatc ccgggtgcac cgaacgctac gtacctgctt
     1141 cgcagtgacg aagaatcgag tcgacgactt gcgtgatcgg tgcggtggcg tcgatcgaga
     1201 tgccgttctg cggaaccgtt tccttcgtgt ggtgcaggcg catgatgatc tctcgttcct
     1261 cttgccttcc gccccaatcg ctggggtcga cgagaacacg ctcgtcaatc cgccgcatca
     1321 cggtgtcgac gtcgtcgact tcgagaacga aaacgccgtc aaggagctcg atgaattgtg
     1381 aagagttcct acagcccccg cagagaaacg tcatgacctc gtcgtggttt gcgatcaggg
     1441 cattgactct tgctacatcc cagatgggcg ggaggcggtt ttccgtgact ccatccgttc
     1501 tggggtgggc ccggagctcc ctgtcgccgt gtacagcgtg atatccgcgt ctctgcagct
     1561 cgttgcagac cgtggtctta ccggtccccg acaggccttc gatcagatag ttcctgacgc
     1621 ccatacagat cgattttgat ctcgtcctct tgtcgcttca agcaagagcg tgaaggaaag
     1681 tggggggccg gtttcccggc cccccacttt gacttcgccg aacgaacgag gcgacctact
     1741 tgtttcgctt ggacttctcg tcgtcctcgt cgatctcgtc cggcgtgatc gcgacgagct
     1801 gatcttcggg gccgaactgc cccgagtgca tccggtgcac cagcaacgtc tcgtcgtgga
     1861 cgtagtccgc gagagcgggg tcccactgct cgacgtagtc ctgcgtcccc tccatcagga
     1921 agtagacgcc ggacggctcc gcgtcacagt ccttgaggct cgcgaaccgc tgcaccgtcc
     1981 gcgcctcttc acgtcgccgg ccctgctgcc cgggagcgtt cggattcccc ggcccctgcg
     2041 ggggcgcggc gatccagatg tcgtcgccgg ccttcttgcc cccggcgacg tcactacccg
     2101 ggtcctcggt gatggcgagg ttgccctctg cgtcgagcgc gaggttgtcc ggcgcgtcga
     2161 agtcggctgc gacgttcgga tcccccgtgg tgttcgtgtg gggcttcgac gcgtcgtaga
     2221 cgtagtggta ggcgaacggg cggttctggt tgtggaggtc gatcgccagc acctcgtcgg
     2281 tgccggtgat cgcgacgtag agcgtgtcgc cgttgccgtt cacgtcgcga cccgtgctcg
     2341 tgtcggtctc gacgtcctcc ggacgctcgt actcgttggc cccgcgctgc tccgcgtggg
     2401 tgtccgagtc gacctgcgac gcctcacggt cgagcggaat ccatcgtcct tctccgtcgt
     2461 ggcggttctg ggtctgcagc gcgtacagct gccccgtcgc gagagggttc tgcttgtcgt
     2521 cgggctcgaa gcggaagatc ccgccggagc gactcgcctg gacgccgcga ctctcggcga
     2581 tgccgtagag gttgagcttg cggtcgaacc gcagtccctc gtgcgatctg gcgcccaccg
     2641 ccggacgagc gcggatgccg tccttcgcgt agtcctgcgt tccgtcctgc ggcgcggtga
     2701 tcggctcgcg ccacggggcc agcctcgtgg ggtcggaggg atccaggaag agctcgtaca
     2761 cgagcccgcc ctgagcctgc gggacggtcg ggtccttgtg tgctgcgacg atcacctctt
     2821 ctgcggcgag cagcgtgttc cacggcgtcc acgcgagccc gtcaaggcgc tcgaagtcgg
     2881 cccgctccgc gatcactccg gtctcgcccg tctggaggtc gaggaacgtg accgcgccgt
     2941 tgttgccggt ctcgtgcgtc cgatagacgt accggcctgc gtccgtcccc gtctcgttct
     3001 gcgtgttcat gtcccacagg tcgtagggga cggtgccggt gccggatccc gccgggggcg
     3061 ggacgagctc ctcgaggatc acctgctgct cgtagccacc gggcagcacg aacggcttcg
     3121 cctcgtagcc agtcggctcg cccgggcacg gcgccgaggc cggcaccggc tggaagtcct
     3181 cgaatggcgt gtagccggct cgggtgtcgc gggcccagcc tccgttcgga gtcgccgcgg
     3241 ccgaaccggt tccgatcaaa gcggcagctc ccactaccac cgccagagtc ttcgtgtgtt
     3301 tcatcgagct cctttctctc cctcgacgag ggacgtagtc gcattcaccg agggcgaacc
     3361 tagagagcga aggtgaacgg gaagttaggt ggggatgacg gcgagatgca gctatcgggt
     3421 gggactccgt cgtgcagcgc gaccctcgtc gaagagccgc gttcctcccg ctgattcacg
     3481 agacgttcat gcgagcgacg tggtgtcttc acggcccact cgtagcctct ccgcgcgtcg
     3541 cgccgcgacg cccgagcagc gcggggacat gagggaggaa gatgcgtgag gaatctcgat
     3601 gagctcggtc cgcgggacga gatggtggcc ggcgtatgcc ggcagctggc cgaggagttc
     3661 acggggatat tcacgccgga gacggtgaag cggttcgtcg cggagtcgtt cgaggcgttg
     3721 tccggcgcga gggtccaagc attcgtcccg ttgttcatcc accgcttcgc aaaggagcgg
     3781 ctcgaggccg tggcgcaagc ggaaggaacg atcttgaaag aggtgcccga ggtcctcttc
     3841 gtatgcgtcc acaacgcggg ccgctctcag atggcagcgg ccctcctgga ccgcgcaggc
     3901 caggggaggg tccacgtccg ctccgccggc tctactccgg cgaacgagat ccacgcaggc
     3961 gtgcgggaag ccatggccga gatcggcatc gacatgaccg gtgagttccc gaagccgctg
     4021 acggacgagg tggtccgggc cgccgacgtc gtcatcacga tggggtgcgg cgacgcctgt
     4081 cccgtctatc ccggcaagcg ctacctcgac tgggagctgc cggatcccgc gaacaagccg
     4141 atcgacgagg tcaggcccat ccgcgaggag atcgaccgcc gggtccgaga gctcctggcg
     4201 gagctcgtgc ccgcgggata gcgcgtacgc aggcgaggga cccgcgactg gtccgtcaag
     4261 tccttttcgg gttcgccttg aacccgggct cgcccagctc ctgcgcggcc tgatccaggt
     4321 cccggggcag gccctcgacg aagcgaccgc ggcggcgaag gttcacggcg gcagcggcgg
     4381 ccgccagcgg cagcgttgcg aggctcggaa ggtccgctcc atgctcgctc aggaaccgcg
     4441 actcttcggg ccgcgcctgc ggaggcatcg ccgcgacggc ccgctgccgg ctgcggcggc
     4501 gggggaacgg gccctcgatc accaaggtca ggccaccgtt gccgctccgc tggaggttca
     4561 ggaacatcgc gcggccgccg gggacgaacg ccggccccgt tgcctcgtct cccggcgtgg
     4621 tgacctcgcc gaagacggag acgtcaccag tcgcgcggtc gatgaactgg atccggttcg
     4681 gatccgacgg gtccggcgtc ggcttgtcct cggtcacgta catgttgcgc gccgcgtcga
     4741 acaccaggtt gtccggcatg cacattcctc cgccggcgtc tccttcggcg aagagctcta
     4801 gacggccgcg gtcgacgtgg agccgccaca ccttcccgca ggcggtctcg tcctgtgtct
     4861 ccgtgaagta gaagtaccgg ccgtcgaagc tgcctccctc gaggcgttgg aacacgacgg
     4921 ctcctgctcg tgctccctgc gtcgccggcg actcgccctc gacgcccagt gggtcgcgga
     4981 tcctcaccca ccggcctttc ccggcgaacg cctcgaggat tcccttgctc aagtctcgcc
     5041 tcttgcgtgg acgcatccgg tagagaagcc cttcccctgc gtcctcggtc aggtagacga
     5101 acccggtctc ggcgtcgacc accgcagtct cgtggtcgaa acgtccgcaa gcgtcgagtc
     5161 ggaccttcgt gtggtcgtgg ggatcgacct cccagatgaa gccgtggtcc gcgtcgagcg
     5221 tcgcggtcga ctcctcgccc gtcagcaccg tgccccatgg ggtgccgctg ccggagcagt
     5281 tcgagaccat gcctcccgcg ttgtacgagc tctcgaggac gttgaagtcg cggtcgagca
     5341 gaaggctcgt gatcgctccc gag
//
LOCUS       DASQDO010000575         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_671777, whole genome shotgun sequence.
ACCESSION   DASQDO010000575 DASQDO010000000
VERSION     DASQDO010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671777"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1499
                     /locus_tag="VEV43_15720"
     CDS             15..1499
                     /locus_tag="VEV43_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYP25016.1"
                     /translation="MPPSHVLNVDLAKIYTRDGERFRYLKTIAWGAELFVREIADDHV
                     RIGVTVRNESGQWRRVDGFIRPPRSSRLKGKDLVVAPEDSRLLTVEFVDVQQGDAAIV
                     ESPSGKVVLVDGGESQLFARYLATRFRDTELTKPKEIDAIVVTHGDADHFEGLPEILE
                     SESLDNGIFIHPRRVFHNGLVKRPSSLPEAEMLGPTTEHDGVTFVTDLHDDLLQVPDE
                     EMNGPFKLWKKVLRTYSERGPVEIKRLAAGDDDELAFLADDGVEVSVLGPLVEDTPGG
                     PGLRFLGTPRDDQELRSQPVRPSHFVGKSASHTINGHSVILQLKFGKARFLFAGDLNA
                     QAEEELVGRHRDGKVDLTSEVLKAPHHGSADFSPGFLEAVAPVVSVVSSGDEESSKEY
                     VHPRATLMGALGRWSRPGVPEPIIFVTELAAFFEKVGPAIPKGGAEADSFFAFERTAI
                     GLVKIRTDGERLLVWTNSAKEDLKEAYAFDLTADPPKRVNLKRA"
     gene            complement(1496..>2736)
                     /locus_tag="VEV43_15725"
     CDS             complement(1496..>2736)
                     /locus_tag="VEV43_15725"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYP25017.1"
                     /translation="RPEVRGRRLSENDVERILKESTDEAERRSAWEASKEIGGVVAGR
                     IRELARLRNQVARDLGFADYYRMSLSLQELPEVWLFDLLDELDELSAGPFAAWKQRLD
                     AELRTRFGVEQLMPWHYADPFFQSLPPAGRVSLDDVLADKSAGALSIETFAAWGIDIA
                     NVMEASDLYPRERKCQHAFCLDVDRSGRDVRILANVVPGERWVEVMLHESGHAAYDVC
                     IDPKLPYLVHRPAHIFVTEAAAILSGRLVRDPEWLVRIAGVDRSRVEELAPRLREATA
                     AQMLLFARWGLVMAHFERELYSDPEADLDARWWDLVERFQLLERPPGRSAPDWAAKIH
                     VAVAPVYYQNYLLGEMLASQLRATCEERFGGLVGVRDAGTFLVDEVFRPGALLRWDDL
                     VEKATGGPLSARAFAADASA"
BASE COUNT          447 a          962 c          945 g          382 t
ORIGIN      
        1 aaaagtagag ggcgatgccg ccctctcacg tgctgaacgt cgacctcgcc aagatctaca
       61 cgcgagacgg cgagcggttc cggtacctca agacgatcgc gtggggggcc gagctgttcg
      121 tccgcgagat cgccgacgac cacgtgcgga tcggtgtcac cgtccgcaac gagagcggcc
      181 agtggaggcg cgtggacggc ttcatcaggc ccccgcgctc cagccgcctg aaaggaaagg
      241 acctcgtagt cgcgcccgag gacagccggc tcctcacggt cgagttcgtc gacgtccagc
      301 agggcgacgc cgcgatcgtc gagtcgccgt cgggcaaggt cgtgctcgtc gacggaggcg
      361 agagccagct gttcgcgcgc tacctcgcga cgaggttccg ggacaccgag ctgacgaagc
      421 cgaaggagat cgacgccatc gtcgtgacgc acggcgacgc ggaccacttc gagggcctgc
      481 ccgagatcct cgagtcggag agcctcgaca acgggatctt catccacccg cgcagggtct
      541 tccacaacgg gctcgtcaag cggccgtcga gcctccccga agccgagatg ctcgggccga
      601 cgaccgagca cgacggcgtc acgttcgtca ccgacctcca cgacgacctc ttgcaggttc
      661 ccgacgagga gatgaacggc ccgttcaagc tgtggaagaa ggtgcttcgg acgtactccg
      721 agcgggggcc ggtcgagatc aagcgactcg cggccggcga cgacgacgag ctcgcgttcc
      781 tcgcggacga cggcgtcgag gtgtcggtgc tcggccccct cgtcgaagac acccccggcg
      841 gccccggcct ccgcttcctg ggcacgccgc gcgacgacca ggagctgcgc agccagcccg
      901 tgcggccgtc gcacttcgtc ggtaagtccg cgtcgcacac gatcaacggc cactcggtga
      961 tcctgcagct gaagttcggc aaggcgcgct tcctgttcgc cggcgacctg aacgcgcagg
     1021 ccgaggaaga gctcgtcggc cggcaccgcg acgggaaagt cgacctgacg tccgaggtgc
     1081 tgaaggctcc gcaccacggg tcggccgact tctcgccggg attcctcgag gcggtcgcgc
     1141 cggtcgtctc ggtcgtctcg tccggcgacg aggaatcgag caaggagtac gtccacccgc
     1201 gcgcgacgct gatgggggcg ctcgggcgct ggtcgcgccc cggcgtgccc gagccgatca
     1261 tcttcgtgac cgagctcgcg gcgttcttcg agaaggtcgg cccggcgatc ccgaaaggcg
     1321 gtgccgaggc ggattcgttc ttcgcgttcg aacggacggc gatcggcctc gtcaagatcc
     1381 gcaccgacgg cgagcgactg ctcgtgtgga cgaacagcgc caaggaggac ctgaaggagg
     1441 cctacgcgtt cgacctcacg gccgacccgc cgaagcgcgt gaacctgaag agggcctagg
     1501 ccgaggcgtc ggcggcgaag gcgcgggcgc tcagcggccc gccggtcgcc ttctcgacga
     1561 ggtcgtccca ccgcagcaac gcgccgggac ggaacacctc gtcgacgagg aacgtccccg
     1621 cgtcgcgcac gccgaccagg cccccgaacc gctcctcgca cgtcgcgcgg agctgcgacg
     1681 cgagcatctc gccgagcagg tagttctggt agtagaccgg cgcgacggcg acgtggatct
     1741 tcgccgccca gtcgggggcc gaccggcccg gcggtctctc gagcagctgg aaccgctcga
     1801 cgaggtccca ccagcgcgcg tcgagatccg cctcggggtc cgagtacagc tcgcgctcga
     1861 agtgtgccat cacgagaccc cagcgagcga acaggagcat ctgggccgcg gtcgcctcgc
     1921 gcagacgcgg cgctagctcc tcgacccggc tccggtcgac gcccgcgatg cgcacgagcc
     1981 actccgggtc gcgcacgagc cgacccgaca ggatcgccgc ggcctcggtc acgaagatgt
     2041 gcgcgggccg gtggaccagg tacgggagct tcgggtcgat gcacacgtcg taggccgcgt
     2101 ggccggactc gtgcagcatg acctcgaccc agcgttcgcc cggcaccacg ttcgcgagga
     2161 tccggacgtc acgccccgag cggtcgacgt cgaggcagaa cgcgtgctgg catttccgtt
     2221 cgcgcgggta gaggtcgctg gcctccatca cgttcgcgat gtcgatcccc cacgcagcga
     2281 aggtctcgat cgagagcgcg cccgccgact tgtctgcgag gacgtcgtcg aggctcacgc
     2341 ggcccgcggg gggcagagac tggaagaacg ggtccgcgta gtgccacggc atgagctgct
     2401 cgacgccgaa ccgcgtccgc agctcggcgt cgagtcgctg cttccatgct gcgaacggcc
     2461 cggcgctcag ctcgtcgagc tcgtcgagga ggtcgaagag ccacacctcc gggagctcct
     2521 ggaggctcag cgacatccgg tagtagtcgg cgaagccgag gtcgcgcgcg acctggttgc
     2581 gcaaccgtgc gagctcccgg atgcgaccgg cgacgacgcc gccgatctcc ttcgacgcct
     2641 cccaagcact acggcgctcg gcctcgtccg tggactcctt caggatgcgc tcgacgtcgt
     2701 tctcgctcag ccggcgaccg cggacctccg gccggt
//
LOCUS       DASQDO010000576        13158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_916234, whole genome shotgun sequence.
ACCESSION   DASQDO010000576 DASQDO010000000
VERSION     DASQDO010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916234"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VEV43_15730"
     CDS             complement(<1..465)
                     /locus_tag="VEV43_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYP25018.1"
                     /translation="MKYGSILDAIGNTPLVEVPRLSPSEDVRLWVKLEGQNPTGSAKD
                     RIALALVEAGEASGEMTPDKTLLEPTSGNTGIALAMVCATKGYRFTAVMPDNASAERV
                     QMLRAFGADIIFSPGEKGSNGAVAMAQEIAGSSEGREKYYMPFQYGNQANADA"
     gene            complement(462..776)
                     /locus_tag="VEV43_15735"
     CDS             complement(462..776)
                     /locus_tag="VEV43_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HYP25019.1"
                     /translation="MPVSVRIPTVFRKFTGNESVVEVEPGTIAGIVDQLEGRYPGLKS
                     QLLTDGGELHRFVNVYVNDEDARYLDKLDTNVSEGDTVSLLPSVAGGAGPASGGPPSS
                     IG"
     gene            1017..2189
                     /locus_tag="VEV43_15740"
     CDS             1017..2189
                     /locus_tag="VEV43_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25020.1"
                     /translation="MPSIAQPIGSVVTEDVSADATSIAVEDAGVFSTHGGEAVFERGT
                     ANEEALTYSGVDLDAHRLIGLTRSNPLTHPSGTFLESVTAAATPTPAPAPSPTTEVPE
                     GTPPPSSGDADETTNGSAGEETEPQDPCEIAAGQSCSDVLGGVISDPPELTCNGDDDD
                     PPVCLDGWIEDPPELTCNGDDDDPPVCLNENLPECNADASCEPILPRTCAPGIQPCHP
                     LPLLPVSGVAVPEAPVVAVAGPDLEEPPVDATDAVDVGAGTCTLPSPHAAFEGFKDSE
                     GVVFGDFFYGVTATCEIRMASMSVAVWIHNHANGEESDSDAGGCSGGGDSACKMAVAS
                     GSHHCDPCNGGWQAHATAVFELRRGKKWDAVPARCSQISKRIITCRSKSEVQKVPA"
     gene            2495..3109
                     /locus_tag="VEV43_15745"
     CDS             2495..3109
                     /locus_tag="VEV43_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25021.1"
                     /translation="MTLLAGTARMCSGAACQGEPLPRIFNASGVPHKSEARLPAGTYR
                     VILIADSTPVKVVLSLDGLTGSRVLRPQTRASVNIIDAADAPPAEGPGVATASTTRDV
                     RENGLLVAVDWWETDHSLATLRGYCLYEGPPSPTWSFSPACSRTGASEAGTSQRLQLP
                     PGGPGYASGTHVRTLPSGSWTFGSYVYSPEVPTGHGATHVWVDY"
     gene            complement(3114..3542)
                     /locus_tag="VEV43_15750"
     CDS             complement(3114..3542)
                     /locus_tag="VEV43_15750"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HYP25022.1"
                     /translation="MNKPDLQMTRALADDMIEHCRAGVPNEACGILGAKDGEIVKVFR
                     MTNASGSPVRYSLDPKEQFAVYNTLDKEGWDLGGVFHSHTHTEAYPSPTDVRLASEDV
                     PYVIVSLAEDPPSVRAFRIEKASWTDTEGEIEEMPVVIVG"
     gene            complement(3539..4564)
                     /gene="selD"
                     /locus_tag="VEV43_15755"
     CDS             complement(3539..4564)
                     /gene="selD"
                     /locus_tag="VEV43_15755"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4520..4522),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYP25023.1"
                     /translation="MDEVRLTSYSHGGGUACKLGTAELAQVLRHVRNTHSDPALLVGL
                     DAPDDAAVYRISDEVALVQTADFFTPVVDDPYDWGRIAAANALSDVYAMGGRPVTALN
                     LVGWPRALDFALLGRVLEGGAAVCAEAGASVVGGHSVDDPEPKFGLSVTGLVHPDRVV
                     RNGDGRAGDALVLTKPLGMGIVSSGIKEGKTSAATAERAVEVMATLNRAASEAMVEAG
                     ASAATDVTGFGLAGHLLEMLGSSLDAELDFDAIPVLEEAFELARAGVLPGGSRRNAEA
                     AGPFVDASGLDEAQRAVLFDAQTSGGLLIAVDEPGRLLAALEARGADGAVVGRLTDGD
                     GHIRVDA"
     gene            4692..5825
                     /locus_tag="VEV43_15760"
     CDS             4692..5825
                     /locus_tag="VEV43_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP25024.1"
                     /translation="MKVLLVDDHAPIRASLRQLLELRPGYQVVGEGANGREAVAAVDA
                     HEPDVVLMDVNMPVMNGVEATRAIKQRHPDVKVLALTAFGDMSLVSASIKAGASGYLL
                     KGGSAEELLNSLQAVAIGQGALDKEVTRGVMDDVAALYRSEQERADALEELDRMKSEF
                     VSVVSHELRTPLTGIMGGVRTLQGGWAQLDDPTKLELLDKIDRQCDRLTHLVDQILTV
                     SGIQGGGLGLAADRFSLTEVGRAVVASLARSFPGREVAFDGSGDVEVAGDRGRIVEAA
                     LALVENALEHTGGRVRVTVTAAGDCARLEIADEGPGIAAALLDRLLTQPFEQGDSSST
                     RRVGGLGLSIYIARRVLEASGGSLVAVSSPGAGSTFTMVVPLA"
     gene            complement(5822..6469)
                     /locus_tag="VEV43_15765"
     CDS             complement(5822..6469)
                     /locus_tag="VEV43_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HYP25025.1"
                     /translation="MLAAALGGLIGFERELSDQPAGFRTHMLVSLGAALFTMAGAYGV
                     ESFFDDTPGRIRFDPTRIAAQVVTGIGFLGAGAIIQQGFSVRGLTTAASLWVTAAVGL
                     SIGLGYYWGAAITSVFTVVALYLLKRARGLLFGRLKRGEVRVVVRMGDNLRLAQLVDV
                     LEMRGSRVDSIKFADDEDESKQLVAKLTLAPDVAAEEVVERASGVPGVRAVDWVR"
     gene            complement(6557..7582)
                     /locus_tag="VEV43_15770"
     CDS             complement(6557..7582)
                     /locus_tag="VEV43_15770"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HYP25026.1"
                     /translation="MRRWTTFLIAVAASMAMAAPALACGGLVAPNGTVNLVRTTTFAG
                     YHDGVEHYVTSFEYAGGGGKFGSIIPLPGIPSDVVRGGDWTLQRLVEEVQPPVARRGL
                     VAFAGASADSAEVVLETRIDALDITVLKGGADEVGKWALDNGFALPPDAPEVLEFYAR
                     RSPIFMAARFDAAEAERLGQGIGDGTPIHLSIPTQNPWVPLRILSLGRQASETVEADV
                     FLLTDARPALLPLPGTTMDLAASEPASKDLLADLRSDKGMEWIPEDDMWLSYLKIDTA
                     AGALDHDLAIDPTGLGTPSKVAAGLVLPEEIVEAAARTPLWAWALAAALVVLTLAFGN
                     RIVMSSR"
     gene            7708..8577
                     /locus_tag="VEV43_15775"
     CDS             7708..8577
                     /locus_tag="VEV43_15775"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HYP25027.1"
                     /translation="MDKKVLAVAIVLVAVVAGACSSDEGDAGSGGADVAAAQVAERGS
                     EGGGGDGLAVGDSDTATQDAYLTSAEPDRVIKTATLELEVDDGRLGSVLREGRVIAEA
                     AGGFLLSTSVEGVERQRGDFAIRVPAGEFEETLSALEELGTVTRENVDGQDVSQEFVD
                     LEARLRNATAQEEVLFRLYDRSTSIADTIRVQRELEDVQLEIERLRGRLRYLSDRTDL
                     STISVSVREAGAVAAGAGPFEKAWDKAKDTFVAVVSGAIVGAGFLLPLLGFALLAYVA
                     FRLLRPRLQRVAP"
     gene            8658..9134
                     /locus_tag="VEV43_15780"
     CDS             8658..9134
                     /locus_tag="VEV43_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HYP25028.1"
                     /translation="MDTPPLHDALRPLGFLLGTWHGRGAGHYPGIDDFEYGEETRFWH
                     YGRPVLAYTQRTWALGSGAPMHSEMGFWRVAGEGVEVMLSHAFGITEILTGTVVGEKV
                     TLRSESLASAPSAKTVEAVARTIEVAGDQLTYDVEMAFGDHALQPHLRATLDRQPE"
     gene            9182..9667
                     /locus_tag="VEV43_15785"
     CDS             9182..9667
                     /locus_tag="VEV43_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25029.1"
                     /translation="MNDFERNKAWADGHRAEIQKVLRQVAARHLEVDTSSFERDTQDA
                     IDYEVAMRERNVACRVRHVTSCGGLRELTLGTSLRGSWSEVMKLAKGHVDWYLYAWSD
                     DDRFVDWMVVDVPRVLAEGLIDRAMEDRREVPLPRSYFVPITVAELARADAIVAASWM
                     P"
     gene            complement(9664..11580)
                     /locus_tag="VEV43_15790"
     CDS             complement(9664..11580)
                     /locus_tag="VEV43_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HYP25030.1"
                     /translation="MRTRGLMWVAAALGAALLAGSMPAAATPVCSDEPRAACGGRIFP
                     EAENSVSFVQHDNGEYEAGILALAEEFPRFVRVTKLSELLGPKAVSVEGREIWLVEIT
                     DFEAPERNKVPVAVSLSVHGPERAGLEGGVRYMEDIARWAAEQPSHKLRNGTEPDSIA
                     VPVSEALKKVHLYLADINPDGWARGDVENGGVFQRGNGNGEDLNREYPTMGWTDVGNT
                     PVSEPETRAWIRLMKHVDPLVSGDLHGELTSANNAFADIMLPAGQWDPVEQAQEERLA
                     RHMASNIERYFEQENIDASDVTGEEGMTPAEYATGFDVVGYDASGFMGDWFTQQGAVD
                     MDVEHFLSHQVPNSTWVPALEKAHIASVRGEIEAMVVEAIATSRVRVSLKLGRVKYLA
                     NGKVVKSSDGYGGPKPPKKVDPLPYNANRLTYFRDLSKHAVRPLRGVSAARLVRRGVG
                     RTQTFVITGAFEKAFKKMPRPKKARRAATQSVKRFVRRGGNLVLTDSALKLLPKLGIV
                     GRSEVQRRVYTAGHVNPTAWDDPYLRKVHHTASQTYYEVPLGYSLADDTSPHWIVMQG
                     AWEEAGGKSIAYVEDEAEIGLGRIKVGKGTVGIFGAILPDPTEKYDHFYGLADYAVSV
                     AGGQILNNMMAYRR"
     gene            complement(11583..11987)
                     /gene="msrB"
                     /locus_tag="VEV43_15795"
     CDS             complement(11583..11987)
                     /gene="msrB"
                     /locus_tag="VEV43_15795"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYP25031.1"
                     /translation="MQEKVQKSEQEWREELTPEQYAILRGKGTEAPFTGKYVHTKDDG
                     VYRCGACGAELFSSDTKFDSGSGWPSFTEPANRANVELHTDTSHGMVRTEVTCARCGS
                     HLGHVFDDGPGPEGKRYCINSCALDLETQSQG"
     gene            complement(12154..12312)
                     /locus_tag="VEV43_15800"
     CDS             complement(12154..12312)
                     /locus_tag="VEV43_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25032.1"
                     /translation="MRVKALLMVAVVSGAFGLVAGAPPAHACTAEFPSLACVYHDVCM
                     VAGPKVCR"
     gene            complement(12426..>13158)
                     /locus_tag="VEV43_15805"
     CDS             complement(12426..>13158)
                     /locus_tag="VEV43_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25033.1"
                     /translation="DATLLMSRYAARRMEAAELESFMARFTSGPVTAPTPVPYYLART
                     SEFPYYEGLLFACGEWASLGWEGVDRMYLRPPASSADVLFPFRYLQDHAVELPRAPSP
                     PGPAWGTTRVRSFGAFDLMVLLENADLTSAGETVPGSHVDAVRGWDGGVLHAWLRGDQ
                     ATVHIGLVDAGVKTRDGRRRRLCGVLSRWVPEAFPGATPARARLPKAEAWKSGGDLVV
                     LRCAGAAVELAKGPSGRAVRAVLGG"
BASE COUNT         1998 a         4596 c         4515 g         2049 t
ORIGIN      
        1 cgcgtcggcg ttcgcctggt tcccgtactg gaacggcatg tagtacttct cccggccctc
       61 cgacgacccg gcgatctcct gcgccatcgc gaccgcgccg ttgctcccct tctcgcccgg
      121 cgagaagatg atgtcggcgc cgaacgcgcg cagcatctga acgcgctccg cgctcgcgtt
      181 gtcgggcatc acggcggtga accgatagcc cttcgtagcg cacaccatcg cgagcgcaat
      241 cccggtgttg ccggaggtcg gttcgagcag ggtcttgtcg ggggtcatct cgcccgacgc
      301 ctcaccggcc tcgacgagcg cgagcgcgat gcggtccttc gccgatcccg tcgggttctg
      361 gccctcgagc ttcacccaga ggcggacgtc ctcggagggg ctgagccgcg ggacctcgac
      421 gagcggcgtg ttaccgatcg cgtcgaggat cgacccgtac ttcacccgat tgagctcggc
      481 ggcccgcccg aggccggtcc ggcgccgcct gctacggacg gcaggagcga gacggtgtcg
      541 ccctcggaca cgttcgtgtc gagcttgtcg aggtagcgcg cgtcttcgtc gttcacgtag
      601 acgttgacga agcggtggag ctcgccgccg tcggtgagga gctgggactt gaggccggga
      661 tagcggccct cgagctggtc gacgatcccg gcgatggtgc cgggctcgac ctcgacgacg
      721 ctctcgttgc cggtgaactt gcggaagacc gtaggaatgc ggacagatac gggcacgacc
      781 gacctccttc ggagccgcca gagcggcgat gctattcgac gtgactcagc gtggcacatg
      841 cgtgacgttt ccggctgcgc gcactgttct tgcttgacta gttatttcgg accctcccat
      901 actggcagag caaccaaatc gtggggattt gggaaaggag ctgggcggtg ggcctgaaga
      961 cttctcgcat tctcggcgcg ctacttctaa cctgcggtgt cgtcgtaagc ctgaacgtgc
     1021 cgtcgatcgc gcaacctatc ggcagcgttg tcacggaaga cgtctcggcg gacgccacgt
     1081 cgatcgcggt cgaagacgcc ggcgtcttct cgacccacgg cggggaggcc gtcttcgaaa
     1141 gaggaactgc caacgaagag gcgttgacgt actcgggcgt cgacctggac gctcacaggc
     1201 tcatcggact gacgcgaagc aatccgttga cgcacccatc cggcacgttc ctcgagtccg
     1261 tcacagctgc cgccaccccc acccctgcgc cggccccatc tccgacgacc gaggtcccag
     1321 aagggacgcc gcctccatct tcgggcgacg cggacgagac gacgaatgga tcggcgggag
     1381 aagagaccga gccccaggat ccatgcgaga tcgcggcggg ccaaagctgc agcgacgtcc
     1441 tcggtggggt tatcagtgat ccgccggagc tcacgtgcaa cggggacgac gacgacccgc
     1501 cggtctgcct cgacggatgg atcgaagatc cgccggagct cacgtgcaac ggcgacgacg
     1561 acgacccgcc cgtgtgcctg aacgagaatc tcccggagtg caacgctgat gcgtcttgcg
     1621 agcccatctt gccgcgcact tgtgctccgg ggatccagcc atgccacccc ctgcccctgt
     1681 tgccggtcag cggcgttgcg gttcccgagg cccccgtggt agccgtcgcc gggcccgatc
     1741 tcgaggaacc ccccgtggac gcgacggacg cagtcgacgt aggtgccggc acttgcactt
     1801 tgccgtcacc gcacgccgcg ttcgaaggct tcaaggactc cgaaggcgtc gttttcggcg
     1861 acttcttcta tggcgtcacc gcaacctgcg agatacgaat ggccagcatg tcggtcgcag
     1921 tttggatcca caaccacgcc aacggcgagg agtccgactc ggatgcgggc gggtgctccg
     1981 gtggcggcga cagcgcatgc aagatggccg tcgcctccgg gtcgcatcac tgtgacccct
     2041 gcaacggtgg ctggcaggcc cacgccacag ccgttttcga gctgcgcagg ggcaagaaat
     2101 gggacgccgt tccggcaagg tgttcgcaga tcagtaagcg aattatcacg tgcaggtcca
     2161 aatccgaagt gcagaaggtc ccggcctagg gggtaaacga aacttggctg tcccgatcgt
     2221 ggtgcgtgcg gcagggcgcc gcttggtttg cgccggattc ctttcgctcc tcgggctggc
     2281 ggtcgtggcc ggcacgcccg tttcggcggc taccgcggga gatgcctcca cgcagcttcg
     2341 tggtgtcaac accttcgtcg ggaacgaggc cggatcgatg acggtgacgt tgccccgcac
     2401 cgcctcggtc gacgcgagtg atggggtacg cgtggaggga gtcggtcgtt tcgccgggct
     2461 ggttttcgtg cgcgatcagc cggggcgaga caaggtgacc ttgctcgccg ggaccgcgcg
     2521 gatgtgctcc ggagcagcat gtcagggcga gcctctgcca cgcatcttca acgcgtcggg
     2581 cgtgccgcat aagagcgaag cgaggctgcc ggccgggact tatcgcgtga tcctcatcgc
     2641 cgactccact cccgtgaagg tggtcctcag tctggacggg ctgacggggt cgcgtgttct
     2701 ccgtccgcaa acccgcgcgt ccgtgaacat catcgacgcc gccgacgcgc cgcctgcaga
     2761 gggacccggc gtggcaaccg cgtcgacgac acgagacgtc cgcgagaacg ggctgctcgt
     2821 tgctgtggac tggtgggaga cggaccattc gctcgccacg cttcgggggt attgcctcta
     2881 cgagggcccc ccgtcgccga cgtggtcctt cagtcccgcc tgcagccgaa ccggcgcgtc
     2941 ggaagccggg acgagtcagc gccttcagct gccacccgga ggcccgggct acgcgtcggg
     3001 gacgcacgtc cggacgctcc ccagcggcag ctggacattc ggttcgtacg tgtattcacc
     3061 cgaggtgccg accggccacg gagcgacgca cgtgtgggtc gactactgat cgctcaaccg
     3121 acgatcacca cgggcatttc ctcgatctcc ccctcggtgt cggtccagga tgccttctcg
     3181 atccggaaag ctcgcactga cggggggtcc tcggccagcg agacgatcac gtacggcacg
     3241 tcctcgctcg ccagccggac gtcggtaggc gacggatacg cctccgtgtg ggtgtgcgag
     3301 tggaacacgc cgccgaggtc ccagccctcc ttgtcgaggg tgttgtagac ggcgaactgc
     3361 tccttcgggt ccagcgagta ccgcaccggg ctgccggacg cgttcgtcat gcggaacacc
     3421 ttgacgatct cgccgtcctt cgcgccgagg atcccgcagg cctcgttcgg gacacccgcg
     3481 cggcagtgct cgatcatgtc gtccgcgagc gccctcgtca tctgcaggtc cggcttgttc
     3541 acgcgtccac cctgatgtgc ccgtctccgt cggtcaggcg tcctaccacg gccccgtcgg
     3601 ccccgcgcgc ctccagcgcg gccaggagcc gccccggttc gtccaccgcg atcaacagcc
     3661 ctcccgacgt ctgcgcgtcg aacagcaccg cgcgctgcgc ctcgtccagc ccggacgcgt
     3721 ccacgaacgg gcccgcggcc tccgcgttcc tgcggctccc gcccggcagc accccggcac
     3781 gggcgagctc gaacgcctcc tccaggacgg ggatcgcgtc gaagtcgagc tctgcgtcga
     3841 gggacgagcc gagcatctcg agcaggtggc cggcgaggcc gaaccccgtg acgtccgtcg
     3901 ccgccgacgc tcccgcctcg accatcgcct ccgaggcggc ccggttcagg gtcgccatca
     3961 cctcgacggc gcgttcggcg gtggccgcgc tcgtcttgcc ctccttgatc ccgctggaca
     4021 cgatccccat gccgagcggc ttcgtcagca cgagcgcgtc gccggcccgg ccgtcaccgt
     4081 tgcgcacgac ccggtcgggg tggacgagcc cggtgacgga aaggccgaac ttcggctcgg
     4141 gatcgtcgac cgagtgaccg ccgaccacgg acgcccccgc ctccgcgcag acggccgcac
     4201 caccttcgag gacccgcccg agcagggcga agtcgagcgc gcgcggccac cccacgaggt
     4261 tgagggccgt gacggggcgg ccgcccatcg cgtagacgtc ggagagcgcg ttggccgcgg
     4321 cgatccgccc ccagtcgtag gggtcgtcga ccaccggcgt gaagaagtcg gccgtctgga
     4381 ccagcgcgac ctcgtccgag atccggtaga ccgcggcgtc gtccggcgcg tcgaggccga
     4441 cgaggagggc cgggtcgcta tgggtgttcc tgacgtgacg cagaacctgc gccagctcgg
     4501 ccgtgccgag cttgcacgct cagcctccgc cgtgcgagta cgacgtcaac cgcacctcgt
     4561 ccattcgggc gctcacctcc tctcgcctct gccgtccggc ccttcgggcc gggcaagtgt
     4621 tccaacaaga ggattggaac agagttcccg cgacgggtga atcgccccgg ttggggaacc
     4681 gaagaacgtt cgtgaaggtg ctcctcgtcg acgaccacgc tccgatccgg gcgagcctcc
     4741 gccagctgct cgagctccgg ccggggtacc aagtggtcgg agaaggggcg aacggccggg
     4801 aggccgtggc cgcggtcgac gcgcacgagc ccgacgtcgt cctcatggac gtgaacatgc
     4861 cggtgatgaa cggcgtcgag gcgacccgcg cgatcaagca gcgccatccg gacgtgaagg
     4921 tgctggcgct gacggcgttc ggcgacatgt cgctcgtgtc cgcgtcgatc aaggcgggcg
     4981 cgtcgggcta cctcctcaag ggcggctcgg ccgaggagct cttgaactcg ctgcaggcgg
     5041 tcgcgatcgg gcagggcgcg ctcgacaagg aggtgacgcg cggcgtcatg gacgacgtcg
     5101 ccgcgctgta ccggtccgag caggagcgcg ccgacgcgct ggaggagctc gaccggatga
     5161 agagcgagtt cgtctcggtc gtctcgcacg agctccgcac tccgctgacc gggatcatgg
     5221 gaggcgtgcg gacgttgcag ggcggctggg cccagctcga cgacccgacg aagctcgagc
     5281 tcctcgacaa gatcgatcgc cagtgcgatc gcctgacgca cctcgtcgac cagatcctca
     5341 cggtgtcggg catccagggc ggaggcctcg gcctcgccgc ggaccgcttc tcgctgacgg
     5401 aggtcgggcg tgccgtcgtc gcgtccctcg cgcgttcctt ccccggacgc gaagtcgcgt
     5461 tcgacggctc cggtgacgtc gaggtggcgg gcgaccgggg caggatcgtg gaggccgccc
     5521 tcgctctcgt cgagaacgcg ctggagcaca cgggcggacg ggtgcgggtc actgtcaccg
     5581 cggcgggtga ctgcgccagg ctcgagatcg ccgacgaggg ccccggcatc gcggccgcgc
     5641 tcctcgaccg gctcctgacg caacccttcg agcaaggcga ctcgtcgtcg acgcgccggg
     5701 tcggcggcct cgggctctct atctacatcg cgcgccgggt gctggaggcg tcgggcggat
     5761 cgctcgtcgc cgtcagctcg ccgggggccg ggtcgacgtt cacgatggtc gtcccgctcg
     5821 cctagcgaac ccagtcgacc gcgcgcactc ccggcacgcc cgaggcgcgc tcgaccacct
     5881 cttctgcggc cacgtccggc gcgagcgtga gcttcgccac gagctgtttg ctctcgtcct
     5941 cgtcgtcggc gaacttgatc gagtcgacgc gggagccgcg catctcgagc acgtctacga
     6001 gctgcgcgag ccggaggttg tcgcccatgc ggacgacgac gcgcacctca ccccgcttga
     6061 gccgcccgaa cagcaggccg cgcgctcgct tcaggagata cagcgcgacg acggtgaaga
     6121 ccgaggtgat agcggccccc cagtagtagc cgagcccgat gctcagcccg accgccgccg
     6181 tcacccatag cgacgccgcg gtcgtcagcc cgcgcacgct gaaaccttgc tggatgatcg
     6241 ccccggcccc caggaaaccg atcccggtca cgacctgcgc ggcgatgcgc gtggggtcga
     6301 agcggatgcg gccgggcgtg tcgtcgaaga acgactcgac tccgtacgcg ccggccatag
     6361 tgaacagcgc cgccccgagc gacacgagca tgtgcgtcct gaagcccgcc ggctggtcgg
     6421 agagctcgcg ctcgaacccg atcaggccac cgagcgcggc cgcgagcacg atacgcaaga
     6481 cgagctcttg ttctgtgagc gtggcccctc ccccggtccc tgcgactcgt cagagcatag
     6541 agaccggagc ggtgcctcag cgcgacgaca tcacgatcct gttcccgaac gccagagtga
     6601 ggactacgag cgcggccgcg agagcccacg cccacaacgg cgtgcgtgcc gcggcctcga
     6661 cgatctcctc ggggagcacc agtcccgccg cgaccttcga cggagtgccg agaccggtcg
     6721 ggtcgatcgc gaggtcgtgg tcgagcgcgc ccgccgcggt gtcgatcttc agatagctca
     6781 gccacatgtc gtcctcgggg atccattcca tccccttgtc ggagcgcagg tcggcgagca
     6841 ggtccttcga cgcgggctcg ctcgccgcga ggtccatcgt cgtgccggga aggggcagca
     6901 gcgcgggccg cgcgtcggtg agcaggaaga cgtcggcctc gacggtctcg gacgcctggc
     6961 ggccgagcga gaggatccgc agcggcaccc acgggttctg cgtcgggatg ctgaggtgga
     7021 tcggagtgcc gtcgccgatg ccctgaccga gtcgctccgc ctccgcggcg tcgaagcgcg
     7081 cggccatgaa gatcgggctg cggcgggcgt agaactcgag gacctccggc gcgtccgggg
     7141 gcagggcgaa gccgttgtcg agcgcccact tgccgacctc gtccgctccg cccttcaaga
     7201 ccgtgatgtc cagggcgtcg atgcgggtct cgaggacgac ctctgccgaa tcggcgctcg
     7261 caccggcgaa ggcgacgaga ccgcggcgcg ctacgggagg ctgcacctcc tccacgaggc
     7321 gctggagcgt ccagtcaccc ccgcgcacca cgtccgacgg gatgcccggc agagggatga
     7381 tcgagccgaa cttgcctccg ccgcccgcgt actcgaacga cgtgacgtag tgctcgacgc
     7441 cgtcgtggta gccggcgaac gtcgtcgtgc gaacgaggtt gaccgtgccg ttcggtgcga
     7501 cgagccctcc gcacgcgagc gccggcgccg ccatcgccat cgacgcggcc actgcgatca
     7561 gaaaggtcgt ccaccgccgc atgtccctct cccctctctc gtccggttgc gagggcttgg
     7621 acggcgcggg tgccgggctg tgttcccggg aacttccgcg ggccgtggcg ccgtccaacg
     7681 cgccataggc gaaagagagg tgccgggatg gacaagaagg tgttggcggt ggcgatagtc
     7741 ctcgtcgcgg tggtcgcggg ggcgtgctcg tccgacgaag gcgacgccgg cagcggcggc
     7801 gcggacgtcg cggcagcgca ggtagcggag cgcgggtccg agggcggcgg gggtgacggg
     7861 ctggcggtcg gggactccga cacggccacg caggacgcct acctgacctc ggccgagccc
     7921 gaccgtgtga tcaagaccgc gaccctggag ctcgaggtcg acgacggacg gctcgggagc
     7981 gtgctgcgcg agggccgtgt catcgccgag gcggccgggg ggttcctgct gtcgacgtcg
     8041 gtcgagggcg tcgagcgcca gcgcggcgac ttcgcgatcc gtgtcccggc gggcgagttc
     8101 gaggagactc tgtccgcgct ggaggagctc gggaccgtga cgagggagaa cgtcgacggc
     8161 caggacgtct cgcaggagtt cgtcgatctc gaggcgcggc tgcgcaacgc gaccgcacag
     8221 gaagaggtcc tgttccgcct ctacgaccgc tcgacgtcga tcgccgacac gatccgagtg
     8281 cagcgcgagc tggaggacgt gcagctcgag atcgagcggc tgcgtggccg cctgcgctac
     8341 ctgtccgacc gcaccgacct cagcaccatt tcggtctcgg tgcgcgaggc cggtgccgtg
     8401 gcggcagggg cgggtccgtt cgagaaggcg tgggacaagg cgaaggacac gttcgtcgcg
     8461 gtcgtgtcgg gggcgatcgt gggggccggg ttcctgctcc cgctgctcgg gttcgcgctg
     8521 ctggcgtacg tggccttccg tctgctgagg ccgcgcctgc agcgggtcgc gccctagcgg
     8581 ccggcgtccc gccccaccac tgaggaaaac tggacgcata gcgtcctttt ttcctcaccg
     8641 gtcgctagag tccgggcgtg gacacgccgc cgctgcacga cgcgctccgg cccctgggtt
     8701 tcctgctcgg gacttggcac ggcagggggg ccgggcacta ccccgggatc gacgacttcg
     8761 agtacggcga ggagacgcgc ttctggcact acgggaggcc ggttctcgcg tacacgcagc
     8821 ggacctgggc gctcggctcc ggggcgccga tgcattcgga gatgggcttc tggcgggtag
     8881 cgggcgaggg cgtcgaggtg atgctgagcc acgcgttcgg gatcacggag atcctgacgg
     8941 ggaccgtcgt aggcgagaag gtgacgctgc ggagcgagag cctggcctcg gccccctcgg
     9001 cgaagaccgt ggaagcggta gcccggacga tcgaggtggc cggcgaccag ctgacgtacg
     9061 acgtagagat ggccttcggg gatcacgctt tacagcccca cctgcgggcg accctggacc
     9121 ggcaaccgga gtagcgaatt gtaatttgag tagttacaac gcccctgacg aacaaggggg
     9181 catgaacgac ttcgaacgaa acaaagcctg ggccgacggc cacagggcag agatacagaa
     9241 agtgcttcgc caagtcgcgg cccggcacct tgaggtggac accagctctt tcgagcggga
     9301 cacccaagac gcgatcgact acgaggtcgc gatgcgtgag aggaacgtcg cctgccgcgt
     9361 ccggcatgtc acctcgtgcg gggggctacg ggagttgacg ctcgggactt cgctgcgcgg
     9421 ctcgtggtca gaagtgatga agctcgccaa gggccacgtc gactggtacc tgtatgcctg
     9481 gtccgacgac gaccgtttcg tcgattggat ggtcgtcgac gtgcctcgcg tcctggcgga
     9541 gggcctgatc gaccgggcta tggaggacag gagggaagtc ccgctcccgc ggagctactt
     9601 cgtgccgatc accgttgcgg agctggcccg ggcggacgcc atcgtcgccg cctcctggat
     9661 gccctagcgg cggtaggcca tcatgttgtt caggatctgg cccccggcta ccgacaccgc
     9721 gtagtccgcc aggccgtaga agtggtcgta cttctcggtc gggtccggga ggatcgcacc
     9781 gaagatccct accgtgccct tgccgacctt gatccggccc agcccgatct ccgcctcgtc
     9841 ctcgacgtac gcaatgctct tgcccccggc ttcctcccac gccccctgca tcacgatcca
     9901 gtgcggcgac gtgtcgtcgg ccagcgagta gcccagaggg acctcgtagt acgtctggct
     9961 agcggtgtgg tggaccttcc gcaggtacgg gtcgtcccac gccgtcgggt tgacgtgccc
    10021 cgccgtgtac acgcgccgct gcacctcgct acgccccacg atgccgagct tcggcagcag
    10081 cttcagcgcg ctgtccgtca gcaccaggtt cccgccccgg cgcacgaagc gcttgaccga
    10141 ctgcgtcgcc gcacggcgcg ccttcttcgg ccgcggcatc ttcttgaacg ccttctcgaa
    10201 cgcaccggtg atcacgaacg tctgcgtgcg cccgacgccc cgccgtacca gccgcgccgc
    10261 cgatacgccc cgcaacggac ggaccgcgtg cttcgacagg tcccggaagt acgtcagccg
    10321 gttcgcgttg tacgggagcg ggtcgacctt cttcggcggc ttcggccccc cgtacccgtc
    10381 cgagctcttg acgaccttgc cgttcgccag gtacttcacg cggccgagct tcagcgacac
    10441 gcggacccgt gacgtcgcga tcgcctcgac gaccatcgcc tcgatctcgc cgcgcacgga
    10501 cgcgatgtgc gccttctcga gtgccggcac ccatgtcgag ttcggcacct gatgcgagag
    10561 gaagtgctcg acgtccatgt cgaccgcgcc ctgctgcgtg aaccagtcgc ccatgaagcc
    10621 cgacgcgtcg tatccgacga cgtcgaatcc cgtcgcgtac tcggctgggg tcatcccctc
    10681 ctcgcccgtc acgtcggacg cgtcgatgtt ctcctgctcg aagtagcgct cgatgttcga
    10741 ggccatgtgg cgggcgagcc gctcctcctg cgcctgctcg acgggatccc actgccccgc
    10801 cgggagcatg atgtcggcga aggcgttgtt cgccgaggtg agctcgccgt gcaggtcgcc
    10861 cgacacgagt gggtcgacat gcttcatcag acggatccac gcgcgcgtct ccggctccga
    10921 cacgggcgtg ttgccgacgt cggtccaccc catcgtcggg tactcgcggt tcaggtcctc
    10981 gccgttgccg ttgccgcgct ggaagacgcc gccgttctcc acgtcaccgc gcgcccaccc
    11041 gtcggggttg atgtcggcga ggtacaggtg gaccttcttc aacgcttcgc tcaccggcac
    11101 cgcgatcgag tccggctcgg taccgttgcg cagcttgtgc gacggctgct cggccgccca
    11161 gcgcgcgatg tcctccatgt agcgcacgcc gccctcgagc cccgctcgct cggggccgtg
    11221 caccgagagc gagaccgcga ccgggacctt gttccgctcg ggcgcttcga agtcggtgat
    11281 ctcgacgagc cagatctcgc gcccttcgac ggacacggcc ttcgggccga gcagctccga
    11341 gagcttcgtc acccggacga agcgcgggaa ctcttcggcg agcgccagga tgcccgcctc
    11401 gtactcgccg ttgtcgtgct ggacgaacga gacggagttc tcggcctcgg ggaagatccg
    11461 gcccccgcac gccgcccgcg gctcgtccga gcagacgggc gtcgccgccg ccggcatcga
    11521 tcccgcgagg agggcggcgc cgagagcggc cgccacccac atgagcccgc gcgtccgcat
    11581 cgtcagccct gcgactgcgt ctcgaggtcg agcgcgcagg agttgatgca gtagcgcttg
    11641 ccctcgggac cgggaccgtc gtcgaacacg tgtcccaggt gcgacccgca acgggcgcaa
    11701 gtgacctcgg tccggaccat cccgtggctc gtgtccgtgt ggagctcgac gttcgcgcgg
    11761 ttcgcgggct cggtgaagct cggccacccg ctgcccgagt cgaacttggt gtcggagctg
    11821 aacagctcgg ccccgcacgc gccgcagcgg tacacgccgt catccttcgt gtggacgtac
    11881 ttgccggtga acggcgcttc cgtccccttg ccgcgcagga tcgcgtactg ctccggcgtc
    11941 agctcctcac gccattcctg ctcggacttc tggactttct cctgcatcgg tggcctcctc
    12001 gcgacccgag agaggaccga cactaacccc ggcctgtacc taccgaagag ctcgcttcgc
    12061 ataccgaagg ccggagaaca ggtggagcgt gcccgtgcct acgacgcgac agcgggcgcc
    12121 cgaaggcgcc cgccggtacg tccgtctgcg gtgctagcgg cagaccttcg gccccgcgac
    12181 catgcacacg tcgtggtaga cgcaggcgag gctggggaac tcggccgtgc acgcgtgcgc
    12241 cgggggcgca ccggcgacca ggccgaacgc gccggagacc actgccacca tcaagagagc
    12301 cttgactcgc atttcccacc tccgttggag aaccggtcga agtgggcgga agataacaac
    12361 caaggcaaac gacgtaaagt ccttggccga agccgtccgc acagttcccc cttctggggg
    12421 agcgttcagc cgcccagcac ggcccggacg gcccgtcccg acggcccctt cgcgagctcg
    12481 accgcagcgc ccgcgcaacg aagcacgacc aggtcgccgc cggacttcca cgcctcggcc
    12541 ttcggaagcc gtgcccgcgc cggcgtcgcg ccggggaacg cctcggggac ccagcggctg
    12601 agcacgccgc agagccggcg gcggcgtccg tcgcgcgtct tcaccccggc gtcgaccaga
    12661 ccgatgtgga ccgtcgcctg gtcgcctcgc agccaggcgt gcaggacccc gccgtcccac
    12721 ccccgcaccg cgtcgacgtg gctgccgggc acggtctcgc ccgcgctcgt gaggtcggcg
    12781 ttctcgagca gcaccatgag gtcgaacgca ccgaaggagc ggacgcgggt cgtcccccac
    12841 gcgggtcccg ggggagacgg cgcacgcggc agctcgaccg cgtggtcctg cagatagcgg
    12901 aaggggaaca gcacgtctgc gctcgacgcc ggcggacgca agtacatccg gtccacgcct
    12961 tcccacccga gcgaggccca ctcgccgcac gcgaacaaca gaccctcgta gtacgggaac
    13021 tcgctcgtgc gcgcgaggta gtacggaacg ggcgtggggg ccgtgaccgg cccggacgtg
    13081 aagcgcgcca tgaacgactc gagctcggcg gcctccatgc gccgggccgc gtacctgctc
    13141 atcagcagcg tcgcgtcg
//
LOCUS       DASQDO010000577        11868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1039048, whole genome shotgun sequence.
ACCESSION   DASQDO010000577 DASQDO010000000
VERSION     DASQDO010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039048"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VEV43_15810"
     CDS             complement(<1..211)
                     /locus_tag="VEV43_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25034.1"
                     /translation="MPLRWVALLVRALAGPPTRAVRGTARRVADPARQVVDHFVSERV
                     TVRQGFVAVLIASLTSLVAGLTLAGM"
     gene            762..1154
                     /locus_tag="VEV43_15815"
     CDS             762..1154
                     /locus_tag="VEV43_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25035.1"
                     /translation="MRRRLSLLLALVLLLSATALAQAAFADVAFAQEIGEGEGESEVG
                     GEESNQQSEGGVSGGDEGEDQEGGGQDEPEAETGSGADQTEEAVEETGPPWTYQMARM
                     SLAALLLLALAIAGAYYRFVVTRQRGAA"
     gene            1177..2253
                     /locus_tag="VEV43_15820"
     CDS             1177..2253
                     /locus_tag="VEV43_15820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HYP25036.1"
                     /translation="MARLIEPGIAAAIARRVAGTPPTEAVDLVGDLERSLQRAVPRSE
                     ELVGAASGIPPPPPVRWAVIDRAAWAEANIAGMSALLDPLVSRITERMAKTPLPIRLA
                     QRGIVSAEVGLLLGYVSRRVLGQYDLLVPETPSRRSRRATVAPGTTLYFVGPNMTETA
                     RRHDFVLDEFSLWVALHEVTHRFQFAGVPWLRPRFFGLVEQYMGSLDVDARGLATRLA
                     GAAREVRARSLPPDQRHPMFLLATPDQRTTLEQLQALMTVVEGHGNFVMDLVGERVIP
                     SFKKMRSTFERRRQQPGPVQRAFNHLIGLEMKLRQYEVGQRFCEEVFRRGGGGAVARL
                     WSSAEELPTLAELREPESWLRRVA"
     gene            2232..3245
                     /gene="tilS"
                     /locus_tag="VEV43_15825"
     CDS             2232..3245
                     /gene="tilS"
                     /locus_tag="VEV43_15825"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HYP25037.1"
                     /translation="MAEAGRVEPGPPALGGPGFDVVTAVRDLIVAADPDDAARHWVVG
                     VSGGPDSVCLLDVLGRLSESLGLELEVAHVDHGLAPGSDETAAAVARLASGAGHDVHV
                     ARASGLEGPNLHARARDFRYRFFETVAEETGATMIVTGHTLDDRVETTLARLVHGAGT
                     EGVAGLRPRDGKRLRPLLGVRRRETRAYCGERGLAFVDDPANEDDRFERAAVRRRLVA
                     AIEERWGDGAVRAVATSAARLAEDADFLKGLAETLYGQLAQRVPGEVAFEMEAFRLAP
                     RALRRRLLALAIGRVRDRSGGIDAALDALDRPDAQLPLSFDVADGATISLTRERVVVK
                     MPR"
     gene            3242..3802
                     /gene="hpt"
                     /locus_tag="VEV43_15830"
                     /pseudo
     CDS             3242..3802
                     /gene="hpt"
                     /locus_tag="VEV43_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
     gene            3864..5807
                     /gene="ftsH"
                     /locus_tag="VEV43_15835"
                     /pseudo
     CDS             3864..5807
                     /gene="ftsH"
                     /locus_tag="VEV43_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
     gene            complement(5921..6649)
                     /locus_tag="VEV43_15840"
     CDS             complement(5921..6649)
                     /locus_tag="VEV43_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HYP25038.1"
                     /translation="MKRTSLVVAALLLLTAAPAGAAPLLDREPDCSQIPPEVQPSPAL
                     ESTPVLPVEVRVMYGPGDRPVVKEHMAVTRGAFERIGIKLRVSYDEIDPPAWLVDPFD
                     GPSNTDILDWMKSHYGGRRPKGTDLVSYMTRYWSGGFADCIGGVAHADRAFALGSIDY
                     AIEGIVPSPTADEGVIAAHELGHLLGAHHHYSNCAEAVPSGAPRGDANPCTTMSPLAT
                     TASTTFGLLEKSYIRHYAESYARG"
     gene            6731..7336
                     /gene="folE"
                     /locus_tag="VEV43_15845"
     CDS             6731..7336
                     /gene="folE"
                     /locus_tag="VEV43_15845"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HYP25039.1"
                     /translation="MSDPGDVVRHMPPRVDRARIEAAVLEILEAVGEDTSRDGLLDTP
                     RRVADMYAEVFSGLGRSAADELDVVFDAGHDEMIMVKDIALHSFCEHHLVPFLGQAHV
                     AYIPNVKGQITGLSKLARLVDLLARRPQVQERLTTQIADAIDSALDPRGVLVVIEAEH
                     LCMTMRGVRKPGSKTVTSAVRGQFRDSEASRSEAMNLIFRG"
     gene            7338..8174
                     /gene="folP"
                     /locus_tag="VEV43_15850"
     CDS             7338..8174
                     /gene="folP"
                     /locus_tag="VEV43_15850"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYP25040.1"
                     /translation="MELLCKGHKVALDHTVLMGVLNVTPDSFSDGGLWLDPEAAIPHA
                     IAMVSEGAGIVDVGGESTRPGADPVPEKDEIARVVPVIEALASQIGVPISVDTRKPAV
                     AEAALEAGASIVNDTSGEEFDPAMDEVVARTGAGLVVMHSRGTPATMRTLTDYEDVVR
                     QTTAFLTERAEQAAEAGVETGAIALDPGIGFAKTREQSLQLLAGLPELVGTGWPVVVG
                     TSRKSFIGTTLGVPEDQRLEGTAATVAWAVTQGAHVVRVHDVEAMARVVRMTEAILAA
                     GR"
     gene            8171..8530
                     /gene="folB"
                     /locus_tag="VEV43_15855"
     CDS             8171..8530
                     /gene="folB"
                     /locus_tag="VEV43_15855"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HYP25041.1"
                     /translation="MSDRILIRGLRVETHVGWTDEERNEPQTVLVDLDVEIDATPSAR
                     SDDLADTVDYAAVISQVSRCVSSREERLLERLAAEVAEMVSAMNGVKRVSVEIAKEVV
                     PVPEEVGRVAVRVERPV"
     gene            8530..9015
                     /gene="folK"
                     /locus_tag="VEV43_15860"
     CDS             8530..9015
                     /gene="folK"
                     /locus_tag="VEV43_15860"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYP25042.1"
                     /translation="MVVSYLGIGSNVGDRVAFCRAAVAALGEHPEIELDATSSLYETS
                     PVGGPPQRSFVNLVVRIRTSLDARGLLEAVKKIERQLGREPNEMRWGPRVADLDILTF
                     DDEKVVEPDLEIPHPRLTQRRFVLVPLLEIDAVAADPGGTRYADALDEAEGEVELLEA
                     F"
     gene            9056..11743
                     /locus_tag="VEV43_15865"
     CDS             9056..11743
                     /locus_tag="VEV43_15865"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HYP25043.1"
                     /translation="MKRILATALAFSGAASVLAALPVSAAGPRGPRVVPGWGTRAPER
                     VQSLPRVALDLPPAAAARAHLSSNRPVYWIADPASTLETVETIEDRAGTTVRFRQVHR
                     GVEVFGAQYLVHLDRSDDGLVPDSANGHYFTEVDVTTSPRIDAASARRLALLWSRPLV
                     PAEVDRHGLTILPVGRGVLAYRFTLWGTRFGRPAKHEVFVNAATGRPVLAYDDLQEDG
                     AVSTSGETVHGDTVPIQAYQRGEVYELRDRSRPMFERDSGEGQITTHNARSTPDYFGT
                     ARNVVTSSSPVFRGVATSTGAVDAHWGAGRVYEFYRALGRNSLDDRGSSIVSTVNGAD
                     PTTFEPMFNAFWDGNQMVYGNPGPIYPLSADLDVVGHELTHGVIDHSADLVYLNQAGA
                     MNEAFADYFGNAIDNEASGIAMDDPGAGLLGEDLCRPENPSPRDWDCPLRDLNDGATT
                     DDYGYFLADFDNGGVHLNATIFGGALWDVREALEPAAADRIVYRALTRFVTPLDDFLD
                     GRAAVVDAARDLGTSDEDVAAIEAAFDSKGIVEGWEEAAGSDARVLVDDVAPLGLLFS
                     PPRVSGRRFVLADYRNQEEMCCEAIELWVGNVDGSGRLQKVGEDTSRATFSDETPAIS
                     GRTVVWAHVVETFGGLEFDVHARTLGGRVRTIAGGPGLQLYPAIDGRTIAWEDGRGRS
                     TDIWVKRGGRKPRALHRARGEQWQPQVAGDWVVWWDVGDGSLVSPASIGMKNVRTGRF
                     VKIRPPSRRSFVGPPAVGPGFVTWYEDRDGNGVGSIVKARLGRTKRTVLVRESAAGAP
                     TWEFGAYALNPPIPSANREYVAYNDELGYVRHFVQQDPDFPVSEVGRDVWIVSAGGGR
                     PQRITTNRGDQAYPLMARGRRVLWLDSAQGHTYLVTRVP"
BASE COUNT         1777 a         4138 c         4208 g         1745 t
ORIGIN      
        1 tcatgccggc gagcgtcagc cctgcgacga gagacgtcag gctcgcgatg aggacggcga
       61 cgaagccctg ccgcacggtg acgcgctcgg acacgaagtg gtcgacgacc tggcgggccg
      121 ggtcggcgac gcgccgggcg gtaccgcgta ccgcacgtgt aggggggccg gcgagggcgc
      181 gcacgaggag ggcgacccac cgcagcggca aggcgagcca ccgcagctga ggccgcctcc
      241 ggtgcgaagc gcgcctgtct ccggcgtctt tcttcattcg cgtgggaact ccaacccgcc
      301 gagcggcgtt cttacgaata atggatgagt tgccgtacga gcttggaagg gtgccaggtt
      361 tcaggcttgg tggcttttgg cagcggcgcc tgagaacacc ggacgaccgg gtttgccctc
      421 cccgagagga gcaccatggg tactaaagag aacgccatcc gcggctcccg cccccaggct
      481 cttccgcgag cctctcagaa gttcccggaa ggaagggtct gcgccgtgca ggggtgcgac
      541 acgcggctct cgacttacaa ccgcagggag aagtgctggg cccacgcgga catgaaggtc
      601 ccgcgtctgc gcggccgcaa gccggccgcc ggctccgcct agtccctccc tcgaggcgca
      661 gcggcgccct cctcccaacc ggggcgccgc ttgcggccct acaccggcgt atatccttcc
      721 aggcgtttcc cgcgacctca cgttggaaag gcaaccggta catgcgacga cgcctgtcac
      781 tgctgctcgc gctggtcctc ttgctgagcg caaccgcgct cgcccaagcc gctttcgcag
      841 acgtcgcgtt cgcccaggag atcggcgagg gcgagggcga gagcgaggtc ggtggtgaag
      901 agagcaacca gcagtccgaa ggcggcgtga gcgggggcga cgagggcgag gaccaggaag
      961 gcggcggcca ggacgagccc gaggccgaga ccggctccgg tgccgaccag accgaggagg
     1021 ccgtggaaga gaccggcccc ccctggacgt accagatggc ccgcatgtcg ctcgccgcgc
     1081 tcctgctgct ggctctcgcc atcgcaggcg cctactaccg gttcgtcgtg acgcgccagc
     1141 gcggcgcggc ctaaccacgc ccgtcctgga gtcggagtgg cgcggctgat cgagccgggg
     1201 atcgcggcgg ccatcgcccg tcgtgtcgcc ggcacccctc ccaccgaggc cgtcgacctg
     1261 gtcggcgacc tcgagcggag cctccagcgc gccgtccccc gcagcgagga gctcgtcggc
     1321 gcggcgagcg ggatcccgcc cccgcccccg gtccggtggg cggtcatcga ccgtgcggcg
     1381 tgggccgagg ccaacatcgc cgggatgtcg gcgctgctcg acccgctcgt gagccgcatc
     1441 accgaacgga tggcgaagac ccccctgcca atccgtctcg cgcagcgggg gatcgtgtcc
     1501 gccgaggtcg ggctgctgct cggctacgtc tcgcgccgcg tgctcgggca gtacgacctg
     1561 ctcgtgccgg agacgccgtc ccggcgctcg cgccgagcga ccgtcgcgcc gggcaccacg
     1621 ctctacttcg tcgggccgaa catgacggag accgcgcggc gccacgactt cgtcctcgac
     1681 gagttcagcc tctgggtcgc gctccacgag gtgacccacc gcttccagtt cgcgggagtc
     1741 ccgtggctgc gcccccgctt cttcggcctc gtcgagcagt acatgggctc gctcgacgtc
     1801 gacgcccgcg ggctcgcgac gcgcctcgcc ggcgcggccc gcgaggtgag ggcgcgttcg
     1861 ctcccgcccg accagcgcca cccgatgttc ctgctcgcga ctcccgacca gcggacgacg
     1921 ctcgagcagc tccaggcact gatgaccgtc gtcgaggggc acggcaactt cgtgatggac
     1981 ctcgtcgggg agcgcgtgat cccgtcgttc aagaagatga ggtcgacgtt cgagcgtcgc
     2041 cggcagcagc ccggccccgt ccagcgggcg ttcaaccacc tcatcgggct cgagatgaag
     2101 ctccgtcagt acgaggtggg gcagcggttc tgcgaggagg tcttccgccg cggaggcgga
     2161 ggcgccgtcg cgcggctgtg gtcgtcggcg gaggagctcc cgacgctcgc cgagctgcgc
     2221 gagccggagt cgtggctgag gcgggtcgcg tagagccggg ccccccggcg ctgggcggcc
     2281 ccggcttcga cgtcgtcacc gccgtccgcg acctgatcgt cgcggcagac ccggacgacg
     2341 cggcgcgcca ctgggtcgtg ggggtctcgg gggggccgga ctcggtctgc ctgctcgacg
     2401 tgctcggccg tctgtcggag tcgctcgggc tcgagctcga ggtcgcccac gtcgaccacg
     2461 ggctggcgcc gggctcggac gagaccgcgg ccgcggtcgc ccggctcgcc tcgggggccg
     2521 ggcacgacgt gcacgtagca cgcgcctcgg ggctcgaagg ccccaacctc cacgcccgtg
     2581 cccgcgactt ccgttaccgc ttcttcgaga ccgtggccga ggagaccggc gcgacgatga
     2641 tcgtcaccgg ccacacgctg gacgaccgcg tcgagacgac gctcgcgcga ctcgtgcacg
     2701 gcgccggcac tgaaggcgtg gccggactgc gcccccgcga cgggaagcgg ctgcgcccgc
     2761 tgctcggggt acgcaggcgc gagacgcgcg cctactgcgg cgagcgcggg ctggcgttcg
     2821 tcgacgaccc cgcgaacgag gacgaccgct tcgagcgagc cgcggtccgc aggcgcctcg
     2881 tcgccgccat cgaggagcgg tggggcgacg gcgcggtccg ggccgtggcg acctcggccg
     2941 cccgcctcgc cgaggacgcg gacttcttga aggggctggc ggagaccctg tacggccagc
     3001 tcgcgcagcg ggtccccggc gaggtcgcgt tcgagatgga ggcgttccgg ctcgcccccc
     3061 gggcgctccg acgccggctg ctcgcgctcg cgatcgggcg ggtccgcgac cgttcgggcg
     3121 ggatcgacgc ggccctcgac gcgctcgacc gaccggacgc gcagctgccg ttgtcgttcg
     3181 acgtcgccga cggcgccacg atctcgctga cccgcgagcg ggtcgtcgtt aagatgccgc
     3241 gatgacagag ctccacgagg acgtcgccga ggtcctcatc cccgccgacg agatccagca
     3301 caagatctcg acgctcgcga aggagatcac cgaggactac cgcggcaagg acctcctgct
     3361 cgtcggcgtg ctcaagggcg ccttcgtgtt catggccgac ctcgcccgcg agatcccgct
     3421 gcccctcgcg ttcgacttca tggcggtctc gtcctacggc tcggcgacga agtcgtccgg
     3481 ggtcgtccgg atcctcaagg acctcgacta cgagatcacc ggccgccacg tcctcttggt
     3541 cgaggacatc atcgactccg gcctgacgat ctcgtacctg cttcgctacc tcgaggcgag
     3601 gagccccgcc tcgctcgaga tctgctcgct gttctggaag aagggctagc aggccgttcc
     3661 gctcgacgtg aagtaccccg gcttcgagat cccgcctgtg ttcgtggtgg gctacggcct
     3721 cgactacgcg gagaagtaca ggaacctccc gtacataggc gtcctcaagc cggctgccta
     3781 cggggccgat tccccttcgt aagccctact acgaagcccg atcggggggt catctagcgg
     3841 cacgtcgctg ctaacctgag ccgatgaatc gaatcttcag atccgtcgtc ttctacgtga
     3901 tcctgatctt cctcgtcgtc tgggtcttca atctctaccg caaccagtcc gaccggcccg
     3961 agaagctcga gtccgtcaac gtcttcgtcg cgcacctcga ggccgacgag atcgagtccg
     4021 ccgagttcca gacgaaggac gagaaggtcg tcggcgagtt cgtctccggc ggccgttacg
     4081 aggtctacct cccccacgac accgtcgacg acttcgccga cctcgcgcag gaccagggcg
     4141 tcgcggtcag cgccgatccg cagcagggca acgtgttcct gtccgtgctg ttccagttcc
     4201 tgccgatcct gatcatcgtc gggttcctgt tcctgctcat gcagcagatg cagggcggcg
     4261 gcaaccgcgt gatgtcgttc ggcaagtcga aggcgcgtct cgtgacgaag gaccagccga
     4321 agatcacgtt cgcggacgtc gccggattgg acgaggccgt agaggagcta caggagataa
     4381 aggagtacct cgccgcgcct cagaagttcc aggcgatggg ggccaagatc ccctagggcg
     4441 tgctgctgtt cggcccgccg ggtacgggca agtccctgct cgcccgcgcg gtcgcaggcg
     4501 aagccggcgt gccgttcttc tcgatctccg gctcggactt cgtcgagatg ttcgtcggcg
     4561 tcggcgccag ccgcgtccgc gacctcttcg agcaggcgaa ggcgaacgcg ccgtcgatcg
     4621 tgttcatgga cgagatcgac gcagtcggcc gtcaccgcgg cgcgggcctc ggcggcgggc
     4681 acgacgagcg cgagcagacc ctcaatcagc tcctcgtcga gatggacggc ttcgacatca
     4741 agggcggcgt gatcctcatc gccgcgacga accggcccga catcctcgac cccgcgctgt
     4801 tgcgcccggg acgcttcgac cgccagatcg tcgtcgaccg cccggacctc aacggccgca
     4861 ccgggatcct caaggtccac acgcgcggca agccgctctc gaaggagatc gacgtcgagg
     4921 tgctagcgcg ccgcacgccg ggcttcaccg gcgcggacct cgcgaacgtg gtcaacgagg
     4981 cggcgctgct cgcggcccgc ttctcgcgca gcgagatcgg catgcgcgag ctcgaagagg
     5041 cgatcgaccg cgtgctcgcg ggcccggagc gccgcagccg cgtgatcaac gagaaggaga
     5101 agaaggtcat cgcgtaccac gaggccggtc acgcgctcgt gagccacgcg ctgccgaacg
     5161 ccgacccggt gcacaaggtg tcgatcgtgt cccgtggccg cgcgctcggc tacacgctga
     5221 cgctcccgac cgaggaccgc ttcctcgtca cgcgttccga gttgatcgac gagctcgcga
     5281 tgctgctcgg cggccgcgtc gcggaagaga tcgtgttcga cgagcccacc acgggggccc
     5341 aggacgacat ccagcgctgc acgcgcatcg cgaagtcgat ggtcaccgag tacggcatgt
     5401 ccgagctcgg ccccctcgcg ctcggccaga ccgagtcgca gccgttcctc ggccgcgact
     5461 tcggccacgt ccaggactac tcgaacgacg tcgcggcgaa gatcgacgaa gaggtccgca
     5521 ggctcgtcga ggaggcccac gccgaggcgc acgagatcct ctcgaagtac cgcgacaagc
     5581 tcgacctcat ggtcgagcgc ctgctcgaga aggagtcgct cgagaaggaa gaggtgtcgg
     5641 agatcctggc cgaggtggcg aagcagagcc cggcgaaccc gctggagcgc acccgcctac
     5701 gccgcgagca gcgcgacgcc gagggcgggt ccgagcagtt gacggggaaa cggtgggggc
     5761 tgccgaagcc gtcgccgaag cctcggttgg gggagaaccc gatttaggtt tccggttggg
     5821 ggcgctcccg aaggcgcccc ctccctcctc cagaccgtct tttagtcgtc gctcgggggc
     5881 gccttcgtcc gctaacgaac cgccgctgcc gggtccgggc ctaaccgcgc gcgtagcttt
     5941 cggcgtagtg ccttatgtag gacttctcta gtaggccgaa ggtcgtcgag gccgtcgtgg
     6001 ctaggggcga catcgtcgtg caggggtttg cgtctcctct cggggctccc gacgggacgg
     6061 cttccgcaca gttcgagtag tggtggtgcg cccccagcag gtggcccagc tcgtgtgctg
     6121 cgatcacgcc ctcgtccgcc gtcggggacg ggacgatgcc ctctatcgcg tagtcgatgc
     6181 tccccagcgc gaaggcccgg tccgcgtgcg ctactcctcc tatgcagtcc gcgaagcccc
     6241 ccgaccagta gcgcgtcatg taggagacga gatccgtccc cttcggcctc cggcctccgt
     6301 agtgggactt catccagtcc aggatgtcgg tgttggaggg gccgtcgaag gggtcgacca
     6361 gccatgcggg cgggtcgatc tcgtcgtagc tcaccctcag cttgatgccg atgcgctcga
     6421 acgcgccgcg ggtcaccgcc atgtgctcct tcacgaccgg gcgatcgccc ggcccgtaca
     6481 tgacccggac ctctacgggc aggaccggcg tcgactccag cgcgggcgac ggctgcacct
     6541 cgggcgggat ctgcgaacag tcgggttcgc ggtccaacag aggcgcggct cccgcgggcg
     6601 cggcggtcag caacagcagg gccgccacga cgaggctcgt cctcttcata ccggcgctgt
     6661 tcggcgaagg gacgcccgtc ccctgcgacg acgcctggca gggaccggac gctcgcctat
     6721 ccttggccgc atgagcgatc ccggtgacgt cgtcaggcac atgcccccgc gggtcgaccg
     6781 cgcccgcatc gaggccgccg tgctcgagat cctcgaggcc gtcggcgagg acacctcccg
     6841 ggacgggctc ctggacaccc cccggcgcgt cgcggacatg tacgcagagg tcttctcggg
     6901 gctgggacgg agcgcggccg acgagctcga cgtcgtcttc gacgccggcc acgacgagat
     6961 gatcatggtc aaggacatcg cgctccactc gttctgcgag caccatctcg tgccgttcct
     7021 gggccaggcc cacgtcgcgt acatcccgaa cgtcaagggc cagataacgg ggctctcgaa
     7081 gctcgcacgt ctcgtcgacc tgctcgcccg ccgcccgcag gtgcaggagc ggctgacgac
     7141 gcagatcgcc gacgcgatcg actcggccct cgacccgcgc ggcgtgctcg tcgtcatcga
     7201 ggcggagcac ctctgcatga cgatgcgcgg cgtccgcaag cccggcagca agaccgtgac
     7261 ctccgcggtg cgcggccagt tccgtgacag tgaggcctcc cgctcggagg cgatgaacct
     7321 gatcttccgg ggctgacgtg gagctcctct gcaaggggca caaggtcgcg ctcgaccaca
     7381 ccgtgctcat gggcgtcctc aacgtcacgc cggactcgtt ctcggacggc gggttgtggc
     7441 tcgaccccga ggcggcgatc ccgcacgcga tagcgatggt gtcggagggg gccgggatcg
     7501 tcgacgtcgg tggcgagtcg acgcgtcccg gggcggaccc ggtccccgag aaggacgaga
     7561 tcgcccgggt cgtcccggtg atcgaggcgc tcgcgtctca gatcggcgtc cctatatcgg
     7621 tcgacacgcg caagcccgcg gtcgcggaag ccgcgctcga agcaggcgcg tcgatcgtca
     7681 acgacacgtc gggtgaggag ttcgaccctg cgatggacga ggtcgtcgcg cgcacggggg
     7741 ccgggctcgt cgtcatgcac tcgcgcggca cccccgcgac gatgcgcacg ctcacggact
     7801 acgaagacgt cgtgcggcag acgactgcgt tcctcacgga gcgcgccgag caggccgccg
     7861 aagcgggagt ggagacgggc gcgatcgcgc tcgatccggg catcggcttc gcgaagaccc
     7921 gcgagcagag cctgcagctg ctggccggcc tgccagagct ggtcgggacg gggtggccgg
     7981 tcgtcgtggg gacgtcgcgc aagtcgttca tcgggacgac gctcggggtc ccggaggacc
     8041 agcggctgga ggggaccgcg gcgacggtcg cgtgggcggt gacacagggc gcgcacgtcg
     8101 tgcgcgtcca cgacgtcgag gcgatggctc gggtcgtgcg aatgaccgag gcgatactag
     8161 cggcgggccg gtgagcgacc gcatcctgat ccggggtttg cgtgtcgaga cccacgtcgg
     8221 ctggacggac gaggagcgca acgaaccgca gaccgtgctc gttgacctag acgtggagat
     8281 cgacgcgacg ccctcggcgc ggagcgacga tctggccgac accgtcgact acgcggccgt
     8341 catctcgcag gtgtcgcgct gcgtgtcttc gcgggaagaa cggctgctcg agaggctggc
     8401 ggccgaggtc gccgagatgg tgtccgccat gaacggggta aagagggtta gcgtggagat
     8461 agccaaagag gtcgtaccgg ttccggaaga ggtgggaaga gtggcagtgc gcgtggagag
     8521 gcccgtctga tggtcgtctc gtacctgggc atcggatcca acgtcggcga cagggtcgcg
     8581 ttctgtcgcg ccgcggtcgc tgcattggga gagcaccccg agatcgagct cgacgccact
     8641 tcgagcctct acgagacgag ccccgtcggc ggcccgccgc agcggtcgtt cgtgaacctc
     8701 gtcgtcagga tccggacgtc gctcgacgcg cggggtctgc tcgaagccgt gaagaagatc
     8761 gagcgccagc tcggccgcga gccgaacgag atgcgctggg ggccgcgcgt cgccgacctc
     8821 gacatcctga cgttcgacga cgagaaggtc gtcgagcccg acctcgagat cccacacccg
     8881 cgcctgaccc agcggcgctt cgtgctcgtc ccgctcctcg agatcgacgc cgtcgcggcc
     8941 gacccggggg ggacgcgcta cgccgacgcg ctggacgagg ccgaagggga agtcgagctg
     9001 ctcgaggcgt tctaggacgc gttagttgtc tcacccagcc cgtaggttgt cgggcatgaa
     9061 gagaatcctc gcgacggcgc tcgcgttctc aggagcggca tcggtgctcg cggctctccc
     9121 ggtctccgcc gccggaccac gcggcccccg cgtcgtcccc ggatggggca ctcgcgcgcc
     9181 ggagcgcgtc cagtcgctcc cgcgcgtcgc gctcgacctc ccgcccgcgg ccgctgcccg
     9241 tgcgcacctg tcgtcgaacc ggcccgtcta ttggatcgcc gatccggcct cgacgctcga
     9301 gaccgtcgag acgatcgagg accgcgcggg gaccaccgtc cggttccggc aggtgcatcg
     9361 aggcgtcgag gtcttcgggg cccagtacct cgtgcacctc gaccgatcgg acgacgggct
     9421 cgtccccgac tcggcgaacg gccactactt caccgaggtc gacgtgacga cgtctccgcg
     9481 catcgacgcc gcgagcgcgc ggcggctcgc gctgctgtgg agccggccgc tcgtccccgc
     9541 cgaggtcgac cggcacgggc tcacgatcct cccggtcggt cgcggggtcc tggcgtatcg
     9601 cttcacgctg tgggggaccc gcttcggacg gcccgcgaag cacgaggtgt tcgtgaacgc
     9661 ggccaccggc cggcccgtgc tcgcgtacga cgacctccag gaggacggcg cggtctcgac
     9721 gagcggtgag accgtccacg gcgacacggt cccgatccag gcttaccagc gcggggaggt
     9781 ctacgagctg cgcgaccgct cgcgcccgat gttcgagcgc gactccggcg agggacagat
     9841 cacgacacac aacgcgcgct cgacgccgga ctacttcggg accgcgcgca acgtcgtcac
     9901 gtcgtcgtcg ccggtcttcc gcggggtcgc gacctcgacc ggcgcggtcg acgcgcactg
     9961 gggagcgggc cgggtctacg agttctaccg cgcgctcggg cggaactcgc tggacgaccg
    10021 cggctcgagc atcgtctcca ccgtcaacgg cgcggacccc acgacgttcg agccgatgtt
    10081 caacgcgttc tgggacggca accagatggt ctacggcaat cccggcccga tctatccgct
    10141 gtcggccgat ctcgacgtcg tcggacacga gctgacgcac ggtgtcatcg accactccgc
    10201 cgacctcgtg tacctcaacc aggccggcgc catgaacgag gcgttcgccg actacttcgg
    10261 gaacgcgatc gacaacgagg cgtccgggat cgcgatggac gacccggggg ccgggctgct
    10321 cggggaggac ctgtgcaggc cggagaaccc gagcccgcgc gactgggact gcccgctgcg
    10381 cgacctcaac gacggcgcga ccaccgacga ctacggctac ttcctcgccg acttcgacaa
    10441 cggcggggtc cacctcaacg cgacgatctt cggcggcgcg ctgtgggacg tgcgcgaagc
    10501 gctcgagccc gcggccgcgg accgcatcgt gtaccgcgct ctgactcggt tcgtgacgcc
    10561 gttggacgac ttcctcgacg ggagggccgc ggtggtggac gcggcgcgcg acctcggaac
    10621 ctccgacgag gacgtcgccg cgatcgaagc cgctttcgac tcgaagggca tcgtcgaggg
    10681 ctgggaggag gcggcggggt cggatgctcg ggtgctcgtc gacgacgtgg cgccgctggg
    10741 gttgctcttc tcgccgcccc gggtgtccgg gaggcgcttc gtcctcgccg actaccgcaa
    10801 ccaggaggag atgtgctgcg aggcgatcga gctctgggtc ggcaacgtcg acgggtccgg
    10861 acggctgcag aaggtcggcg aggacacgtc gcgggcgacg ttcagcgacg agacgccggc
    10921 gatctcggga cggaccgtcg tgtgggccca cgtggtcgag acgttcggcg ggctcgagtt
    10981 cgacgtccac gcccggacgc tcggcgggcg cgtgcggacg atcgcagggg ggccggggct
    11041 ccagctctat ccggcgatcg acggccggac catcgcgtgg gaggacgggc gcggccgctc
    11101 tacggacatc tgggtcaagc gcggcgggcg caagccacgc gcgctgcacc gcgcccgcgg
    11161 cgagcaatgg cagccgcagg tcgcgggtga ctgggtcgtg tggtgggacg tcggcgacgg
    11221 gagcctcgtg agccccgcgt ccatcgggat gaagaacgtg cggacgggac ggttcgtgaa
    11281 gatccggccc ccgtcgcgcc gttcgttcgt ggggccgcct gccgtcggcc ccggcttcgt
    11341 cacgtggtac gaggaccggg acggcaacgg agtcggctcg atcgtgaagg cgcggctcgg
    11401 ccggacgaag cggacggtgc tcgtgcgcga gtctgccgcc ggcgcgccga cgtgggagtt
    11461 cggcgcgtat gcgctgaacc cgccgatccc gtcggcgaac cgggagtacg tcgcctacaa
    11521 cgacgagctc ggctacgtgc gtcacttcgt gcagcaggac ccggacttcc ccgtgagcga
    11581 ggtcgggcgc gacgtgtgga tcgtgagcgc cggcgggggc cggccgcagc ggatcacgac
    11641 gaaccgcggg gaccaggcgt acccgctgat ggcgagaggc cgccgcgtcc tgtggctgga
    11701 ctcggcgcag gggcacacgt acctcgtcac ccgcgtgccc tagctccggg gctgcccgcc
    11761 cttaggattg cggccgaccg gtacccgact acgacggatc gcctcggcga tccgccacga
    11821 ctacgacgga tcgcctcggc gatccgccac gacaaacgga ctggatcc
//
LOCUS       DASQDO010000578         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1100799, whole genome shotgun sequence.
ACCESSION   DASQDO010000578 DASQDO010000000
VERSION     DASQDO010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100799"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VEV43_15870"
     CDS             complement(<1..1127)
                     /locus_tag="VEV43_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYP25044.1"
                     /translation="MIELTDVQKTIVETVRDFVDNEVIPVADEMEHRDEYPEKIVEGM
                     KEMGLFGLTVPEEYDGVGESLTTYALTVVELARGWMSLSGVLNTHFMGCYLINKFGTD
                     EQKQRLLPKMATGEMRAALAMSEPEAGSDVQAIRTKAVRDGDHYVLNGQKMWLTNGLR
                     SSIVMTLVKTDPEADPPHRGMSMLIVEKEPGVGQPAPGLDVGKNIEKLGYKGVETTEM
                     TFSDFRIPVSSLLGTEEGHGFRQVLDGIEVGRVNVAARAVGVATRAFEEAIRYSQERK
                     AFGKQISGHQAIQIHLAEMATTLEAARLMMLQAAVKKSAGERSDLEAGMAKLFASEAC
                     HEITLKSLRVHGGYGYSKEFTVERLYRDAPFLLIGEGPSEI"
     gene            complement(1124..2074)
                     /locus_tag="VEV43_15875"
     CDS             complement(1124..2074)
                     /locus_tag="VEV43_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HYP25045.1"
                     /translation="MRARRSCLSVPGSSPKMLAKGPTLPADMVFMDLEDSVAPLAKEE
                     ARGNVVDALKNNDWGDKTVVVRINSIDTQWAADDLKTVVEAAGSHLDCVMIPKVQSSD
                     EVKFVDHMLRMIEANTGLDKRIGIEAQIETATGLKNVYDIAHASDRMETLIFGPADMS
                     ASLGLPTVTAGLPMPGYPGDHWHWVLETILVAARDAGLQAIDGPYLLIKDLDGFREMA
                     TRARSLGYDGKWALHPGQIDVLNEVFTPTQDEYDKAEALLEAYKHATEVDRRGAVMFG
                     NEMIDEASRKMAVQFAERGRAAGMTPAKTLADFEKEWANQ"
     gene            complement(2092..>2992)
                     /locus_tag="VEV43_15880"
     CDS             complement(2092..>2992)
                     /locus_tag="VEV43_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYP25046.1"
                     /translation="KQCRAVYQQLDDGLAKLLERTNDDDLVIFMSDHGHQACTRAVTM
                     DRVLQHLGYLEFGRGSFAFNLIRWGPGRRIARRIYDILKLHGRISIPASPIEWSKTKA
                     YTSVVSTGEGVSVNLKGREPGGTVDPKDFEKVREEVKQALEAFRDPGNGKPPISKIYR
                     KEEILHGEFLDTAPDLLLVPAPFYSLTHAKGMVEEADWMSGDHRIEGVIVATGPEVTP
                     GPLKETAELIDLGPTSMAALGVPSAIPRDGKVLESLIGPGKSLEVRSDGTATRASTVD
                     ESGLNESEEGEIEDHLRGLGYVE"
BASE COUNT          427 a         1033 c          967 g          565 t
ORIGIN      
        1 tggatttcag acggtccctc accaatcaga agaaatggtg catcacggta aagacgctca
       61 acagtgaact ctttggaata tccgtacccc ccatggacac ggagggattt gagcgtgatc
      121 tcgtggcaag cctcggacgc gaacagcttc gccatgcctg cctcgaggtc cgatcgctcc
      181 cccgccgact tcttcacggc cgcctggagc atcatcagcc gggccgcctc gagcgtcgtc
      241 gccatctccg cgaggtggat ctggatcgcc tggtggcccg agatctgctt gccgaacgcc
      301 ttgcgctcct ggctgtaccg gatggcctcc tcgaaggccc tggtcgccac cccgacggcc
      361 cgggccgcga cgttgacgcg gccgacctcg atcccgtcga ggacctggcg gaagccgtgg
      421 ccctcctcgg tccccaatag cgaggagacc gggatccgga agtccgagaa cgtcatctcg
      481 gtcgtctcga cgcccttgta gccgagcttc tcgatgttct tgccgacgtc gagcccgggt
      541 gccggctgtc ccacccccgg ctccttctcg acgatcagca tgctcatccc gcggtggggc
      601 gggtcggcct cggggtcggt cttgacgagc gtcatcacga tgctcgagcg aaggccgttc
      661 gtcagccaca tcttctggcc gttgagcacg tagtggtcgc cgtcgcgcac ggccttcgtc
      721 cggatcgcct ggacgtcgga gccggcctcg ggctcggaca tcgcgagcgc ggcccgcatc
      781 tcgcccgtcg ccatcttcgg cagcagccgt tgcttctgct cgtcggtgcc gaacttgttg
      841 atgagatagc agcccatgaa gtgcgtgttg aggacacccg agaggctcat ccatccccgg
      901 gcgagctcga cgaccgtgag ggcgtacgtc gtgagcgact cgccgacgcc gtcgtactcc
      961 tccgggactg tgaggccgaa cagccccatc tccttcatcc cctcgacgat cttctcgggg
     1021 tactcgtcgc ggtgctccat ctcgtcggcg acggggatga cctcgttgtc gacgaagtcg
     1081 cggacggtct cgacgatggt cttctggacg tcggtgagct cgatcactgg ttcgcccact
     1141 ccttctcgaa gtcggccagc gtcttggcgg gagtcatccc cgcggcacgg ccccgctcgg
     1201 cgaactgcac cgccatcttg cgcgacgcct cgtcgatcat ctcgttgccg aacatcacgg
     1261 cgccgcggcg gtcgacctcg gtcgcgtgct tgtacgcctc gagcagggcc tcggccttgt
     1321 cgtactcgtc ctgcgtcggc gtgaagacct cgttgaggac gtcgatctgc ccggggtgga
     1381 gcgcccactt gccgtcgtag ccgagcgacc gggcgcgcgt cgccatctcg cggaacccgt
     1441 cgaggtcctt gatcagcaag tagggaccgt cgatcgcctg caggccggcg tcacgggccg
     1501 ctacgaggat cgtctcgagc acccagtgcc agtggtcgcc cggatacccg ggcatcggga
     1561 gccccgccgt caccgtgggg aggccgagcg acgcgctcat gtcggccgga ccgaagatca
     1621 gcgtctccat ccggtccgat gcgtgcgcga tgtcgtagac gttcttgagc ccggtcgcgg
     1681 tctcgatctg ggcctcgatc ccgatgcgtt tgtccaggcc ggtgttcgcc tcgatcatcc
     1741 gcagcatgtg gtcgacgaac ttcacctcgt cggagctctg gaccttcggg atcatgacgc
     1801 agtcgaggtg gctccccgcg gcctcgacga ccgtcttcaa gtcgtccgcg gcccactgcg
     1861 tgtcgatcga gttgatgcgg acgacgacgg tcttgtcgcc ccagtcgttg ttcttcaggg
     1921 cgtcgacgac gttgccgcgc gcctcttcct tcgcgagggg cgccacggag tcctcgaggt
     1981 ccatgaacac catgtcggcg gggagcgtcg gccccttcgc cagcatcttc gggctcgagc
     2041 ccggcaccga tagacaagac ctgcgcgcgc gcaagagaac ctcctgtgtc gttactcgac
     2101 gtagccgagg ccacgcaggt ggtcctcgat ctctccttct tcggattcgt tcaggccgga
     2161 ctcgtccacg gtgctcgccc gcgtcgcggt gccgtcggag cggacctcca gcgacttgcc
     2221 cggcccgatg aggctctcga ggaccttgcc gtcgcggggg atggcgctcg ggacgccgag
     2281 cgcggccatc gacgtcgggc cgaggtcgat cagctcggcc gtctccttca gcgggcccgg
     2341 cgtgacttcc ggtcccgtcg cgacgatcac gccctcgatg cggtggtcac ccgacatcca
     2401 gtccgcctcc tcgaccatgc ccttcgcgtg cgtcagcgag tagaacggcg ccggcacgag
     2461 cagcaggtcg ggggccgtgt cgaggaactc gccgtggagg atctcctcct tgcggtagat
     2521 cttcgagatc ggaggcttgc cgttgcccgg gtcgcggaac gcctcgagcg cctgcttgac
     2581 ctcctcgcgg accttctcga agtccttcgg gtcgacggtg ccaccgggct ctcggccctt
     2641 gaggttgacc gagaccccct cgccggtcga gacgacgctc gtgtaggcct tggtcttgct
     2701 ccattcgatc ggcgacgccg ggatcgagat ccggccgtgc agcttcagga tgtcgtagat
     2761 ccggcgggcg atgcgccggc ccggccccca gcggatcagg ttgaaagcga acgagccgcg
     2821 tccgaactcg aggtagccga ggtgctggag cacgcggtcc atcgtcaccg cgcgcgtgca
     2881 cgcctggtga ccgtggtccg acatgaagat cacgaggtcg tcgtcgttcg tccgctcgag
     2941 cagcttcgcg agaccgtcgt cgagctgctg gtacaccgcg cggcactgct tc
//
LOCUS       DASQDO010000579         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_733340, whole genome shotgun sequence.
ACCESSION   DASQDO010000579 DASQDO010000000
VERSION     DASQDO010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733340"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEV43_15885"
     CDS             <1..800
                     /locus_tag="VEV43_15885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYP25047.1"
                     /translation="VDVLGGLKLILDQGYSREDVLDVARAYGQTMSRLAQAEVRLFNK
                     VFIAPLREQAVDDDTLAGQLEAVVPALLELLGKVIHHVHRRHLAIALQEVTATERPGS
                     TETLAAGFVDLVDFSRVTQELEGEDLGHLVNRFETMAIETCVAGGAHVVKMIGDAVMF
                     VAPNVTTALRVALAVVAGVEEDEALPAARAGLDFGEVVPMSGDFFGRPVNVAARLTSF
                     ARPGTVVVSEALTESAGVALETSHIGRVRLHNVGHVRAFKVNAVAGP"
     gene            864..1829
                     /locus_tag="VEV43_15890"
     CDS             864..1829
                     /locus_tag="VEV43_15890"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HYP25048.1"
                     /translation="MTPTTLDERPRAEAAPAPTPGVRPLEPKMVRLQRRLVLAVTIFP
                     FLGFVAAVWTFWGTGLTLLNAALFLSFYVFTGLGITVGFHRYLTHGGFETKPWVRAGF
                     AIAGSMAIQGSVIAWVSDHRRHHAFSDRPGDPHSPHLEDGPGVKGVVKGLWHAHMGWL
                     FKEELTSPQRWAPDLWKDPMMRWIDKYFPYWAVASFLLPPAIGGLVTGTWWGALTAFL
                     WASLARIFLLHHVTWSINSICHFYGKRPYETPDHSTNNWVLSLISFGESWHNNHHAFP
                     TSAVHGIERGQIDPSGATIRLLQKLRLARDVKVVSPKQLAAKRAP"
     gene            1859..2464
                     /locus_tag="VEV43_15895"
     CDS             1859..2464
                     /locus_tag="VEV43_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYP25049.1"
                     /translation="MTGQERRAQLLDVGRAVFAERGFEAASVEEIAARANITKPVVYE
                     HFSGKEGLYAVIVDREVQSLLGRITESLEGSHPRALIEQAAVAFLGYIEAEPEGFRIL
                     VRDSPVASATGTLASVIGDIAMQVEYILRNQFAERGFDTKLSPLYSQALVGMVALVGQ
                     WWLDAGRPQKDIVAAHLVNLAWNGLGGLEANPRLKREGTGA"
     gene            2649..>4089
                     /locus_tag="VEV43_15900"
     CDS             2649..>4089
                     /locus_tag="VEV43_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HYP25050.1"
                     /translation="MGSRIRAALVAALVAAGALVVVAPPAAAAATLTVTTTTWDIVGL
                     DSNKPSVSNGRPNEFPVGAKVCNTGDAAATSVQTAFNWTTANANLSLANATTTRSLGT
                     LAPGACAHATYNVVINRNQQSFNSRTRGYQITATAAGGLTASTPANREVYVEKLVSQS
                     RNSVIGITGEACTGGSCTVYRGQTYRFTLTSKTATQGYEQLETFVNFPDSIFEIQDIK
                     TTYAAPARARNDKVYADACGWDAVTTSPTYRSCVGPTKFTGGKAGGNPITTVYTVKVV
                     GTGSGTLSGLVYDFSGSSFHYNADYGTGVNAVPFTAADAADLSLTKTHTGAFVRGGTG
                     TYRLTVSNSGPATSGAVTITDTLPAGLTYRSFSGTGWSCSGCSSASQTVTLTRAGIAT
                     GASSFVDLPVDVGAGAAASLTNNASVSQVTSATLNDPAPANNIASDPTTTANPGEAAL
                     VVTKTRDVVMTPGTEETNTIRVRNDGPGTV"
BASE COUNT          665 a         1464 c         1356 g          604 t
ORIGIN      
        1 acgtagacgt gctggggggc ctgaagctga tcctcgacca gggctactcg cgcgaagacg
       61 tcctcgacgt cgcgcgcgcg tacggccaga cgatgtcgcg cctcgcccaa gccgaggtgc
      121 gcctcttcaa caaggtcttc atagccccgc tgcgcgaaca agccgtggac gacgacacgc
      181 tcgcggggca gctcgaagcc gtcgtccccg cgttgctgga gctgctcggc aaggtcatcc
      241 accacgtgca ccggcgccat ctcgccatcg ctttgcaaga ggtgaccgcg accgaacgcc
      301 cgggttcgac cgagacgctc gcggcgggct tcgtcgacct cgtcgacttc tcgcgcgtaa
      361 cgcaggagct cgagggagag gacctcggcc acctcgtcaa caggttcgag acgatggcga
      421 tcgagacgtg cgtcgccggc ggcgcgcacg tcgtcaagat gatcggcgac gcggtgatgt
      481 tcgtggcgcc gaacgtcacg accgcgctcc gggtcgcgct ggcggtcgtc gccggcgtcg
      541 aggaggacga ggcgctcccc gccgcgcgcg cagggctcga cttcggcgag gtcgtgccga
      601 tgagcggcga cttcttcgga cgccccgtga acgtcgcggc gcggctgacg tccttcgccc
      661 ggcccgggac cgtagtcgtg tccgaggccc ttacggaatc agcgggcgtc gcgctcgaga
      721 cgagccacat cgggagggtc cgcctccata acgtcgggca cgtcagagct ttcaaggtga
      781 acgccgtcgc cgggccctag gccctacgcg gtggtaactt actcctacgt aaccgtaggc
      841 aacccgagct cagggagatt tcgatgacgc ccaccacgct ggacgagcgg ccgcgcgccg
      901 aggccgcccc cgcgcccact cccggagttc gcccgctcga accgaagatg gtccggctgc
      961 agagacggct cgtcctcgcg gtgaccatct tcccgttcct cggcttcgtc gccgcggtgt
     1021 ggaccttctg gggtaccggg ctgacccttc tcaacgccgc gctgttcctg tccttctacg
     1081 tcttcaccgg cctgggcatc acggtgggtt tccaccgcta tctcacgcac ggcgggttcg
     1141 agacgaagcc gtgggtgcgc gccggcttcg cgatcgcggg gtcgatggcg atccagggct
     1201 cggtcatcgc gtgggtgtcg gaccaccgac ggcaccacgc gttctccgac cgccccggcg
     1261 acccgcactc cccccacctc gaggacggcc cgggcgtcaa gggcgtcgtg aaagggctgt
     1321 ggcacgcgca catggggtgg ttgttcaagg aggagctgac gtcgccgcag cggtgggccc
     1381 cggacctgtg gaaagacccg atgatgcggt ggatcgacaa gtacttcccg tactgggcgg
     1441 tggcgtcgtt cctgctcccg cccgcgatcg gcggcctcgt caccgggacg tggtggggcg
     1501 ccttgacggc gttcctgtgg gcgagcctcg cgcgcatctt cctgctgcac cacgtgactt
     1561 ggagcatcaa ctcgatctgc cacttctacg ggaagcgccc gtacgagacg ccggaccatt
     1621 cgacgaacaa ctgggtgctc tcgctgatca gcttcggcga gtcgtggcac aacaaccacc
     1681 acgcgttccc gacgtcggcc gtgcacggca tagagcgcgg ccagatcgac ccgagcggcg
     1741 caacgatcag gctgctccag aagctgcgtc tggcgcggga cgtgaaggtc gtctcgccca
     1801 agcagctcgc agcgaagcgc gctccctgag tcccgcgggg aaggcacccc gggtgcgcat
     1861 gaccgggcag gagcggaggg cccagctcct cgacgtcgga cgggcagtgt tcgccgaacg
     1921 cgggttcgag gccgcgtcgg tcgaggagat cgccgcgcgg gcgaacatca cgaagcccgt
     1981 cgtctacgag cacttcagcg gcaaggaggg cctgtacgcg gtcatcgtcg accgcgaggt
     2041 ccagtcgctg ctcggccgga tcaccgaatc gctcgagggg tcgcacccgc gcgctctgat
     2101 cgagcaagcg gccgtcgcct tcctcggcta catcgaggcc gagccggagg ggttccgcat
     2161 cctcgtgcgc gactcccccg tggcgagcgc gaccgggacg ctggcgagcg tcatcgggga
     2221 catcgcgatg caggtcgagt acatcctgcg caaccagttc gcggagcggg gtttcgacac
     2281 gaagctctcg ccgctctact cccaggccct cgtggggatg gtcgccctcg tcgggcagtg
     2341 gtggctggac gccgggcgac cgcagaagga catagtggcg gcgcacctgg tgaacctggc
     2401 ctggaacggc cttgggggcc tggaggccaa ccctcgactg aaacgtgagg gtacgggggc
     2461 ctagccgcgc agctccttgc gccgcacggg cttcagcccc gaccggtcgt cgctaatcgc
     2521 caccttcgtc gaccctgacc tatcgctgcg tcgaatacgt acctaaccct caccccccac
     2581 cgtccgatga acccctaacg ggagatcgcg ggctagtcat gccccgcaac ggagctggga
     2641 ggctttgaat gggttcgagg atccgcgcgg cactggtcgc cgcgctagtc gcggcgggcg
     2701 cgctcgtcgt cgtcgcgccg cctgctgcgg ccgcggcaac gctcacggtc acgaccacga
     2761 cgtgggacat cgtcggtctc gactcgaaca agccgtcggt ctcgaacggc cgcccgaacg
     2821 agttccccgt cggcgcgaag gtctgcaaca ccggcgacgc cgccgcgacc tcggtccaga
     2881 ccgcgttcaa ctggacgacg gcgaacgcga acctctcgct cgcgaacgcc acgaccacgc
     2941 gttcgctggg cacgctcgct cccggagcct gtgcccacgc gacctacaac gtcgtgatca
     3001 accgcaacca gcagtcgttc aacagccgca cccgcggcta ccagatcacc gcgacggccg
     3061 ccgggggcct gaccgcgtct acgccggcga accgcgaggt ctacgtcgag aagctcgtct
     3121 cgcagagccg caactcggtc atcggcatca ccggcgaggc gtgcaccggc ggcagctgca
     3181 ccgtgtaccg gggccagacg tatcgcttca cgttgacgtc gaagaccgcg acgcagggct
     3241 acgagcagct cgagacgttc gtgaacttcc ccgactcgat cttcgagatc caggacatca
     3301 agacgacgta cgcggccccc gcgcgcgccc gcaacgacaa ggtgtacgca gacgcatgcg
     3361 gctgggacgc cgtcacgacg agcccgacct accgcagctg cgtcggcccg accaagttca
     3421 ccggcggcaa ggccggcggc aacccgatca cgaccgtcta cacggtgaag gtggtcggga
     3481 ccggcagcgg gacgctctcc ggcctcgtct acgacttctc gggcagcagc ttccactaca
     3541 acgcggacta cggcacaggc gtgaacgcgg tgcccttcac cgccgccgac gcggcggacc
     3601 tgtcgctgac gaagacccac accggagcgt tcgtgcgcgg tgggaccggc acgtaccgcc
     3661 tcacggtgag caactccggc cccgcgacct cgggcgccgt cacgatcacc gacaccctcc
     3721 cggcgggcct gacctaccgg tctttcagcg ggaccggctg gagctgctcc ggctgcagct
     3781 ccgcctcgca gaccgtcacg ctgacccgcg ccgggatcgc gaccggcgcg tcgtcgttcg
     3841 tcgacctccc ggtcgacgtc ggcgcgggag cggcagcttc gctaaccaac aacgcgtcgg
     3901 tctcgcaggt gacgagcgcg accttgaacg acccggctcc ggccaacaac atcgcatcgg
     3961 accccaccac cacggcgaac ccgggcgagg ccgccctcgt cgtcacgaag acccgcgacg
     4021 tcgtgatgac gccggggacc gaggagacca acacgatccg ggtccgcaac gacggtcccg
     4081 gcaccgtcc
//
LOCUS       DASQDO010000580         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_1101042, whole genome shotgun sequence.
ACCESSION   DASQDO010000580 DASQDO010000000
VERSION     DASQDO010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101042"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2029
                     /locus_tag="VEV43_15905"
     CDS             <1..>2029
                     /locus_tag="VEV43_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HYP25051.1"
                     /translation="KQSLFARSEASELMDGAADFGGGGGGGDDATPRGAGFVEPTVRK
                     NFADTALWVANLGVKDDGVAEVELDMPENLTTWKARVWVMSAGTRVGQGQAEIVTAKD
                     LIVRLQAPRFFVQKDEVVLSANVHNYLKSPKRAEVTLQLEGGCLEPLVGEGKTGGLVN
                     EPNLAASTLTQTVHVEPGGEQRVDFRVRVASPGSATVRMLARTDEESDATQMSFPVYV
                     HGMLKTESFAGAIRPSASAASVSFTVPQERIPEQSRVEIRYSPSVAGAMVDALPYLTD
                     FPYGCTEQTLNRFLPTVITQRVLIAMGLNLKDIAAKRTNLNAQEIGDDRARAAARQNG
                     TRLNPVFDEAVVLAMTRAGVTKLANQQLPDGGWGWFSGYGEQSGPHTTATVVHGLQIA
                     RANDVALPPGVLERGVEWLKLYEARQVQMLKNAPGNVSPSKNASDNLDAFVFMVLSDA
                     GHVNGDMREFLYRDRSSLSVYCLAQLGLALHRQKGQEEKLAMVLRNLSQYVVQDEENQ
                     TAYLKLPESGRYWWYWYGSDTEANAYYLKLLAKTDGKGELPARLAKYLVNSRRNASYW
                     NSTRDTALCVEALADFIAASGEGKPDMKVAVQVDGKVVKEVAINAANLFSFDNKVVLE
                     GPEVTPGAHRIEFTRTGTGPLYFNAYVTNFTLEEPITKAGLEIRVQRKYYRL"
BASE COUNT          358 a          693 c          705 g          273 t
ORIGIN      
        1 gaagcagtcg ctgttcgccc ggtccgaggc gagcgagttg atggacggcg cggccgattt
       61 cggcgggggc ggcggcggcg gtgacgacgc gacgccgcgg ggcgccgggt ttgtcgagcc
      121 caccgtccgc aagaatttcg ccgacaccgc cctctgggtc gcgaacctcg gcgtgaagga
      181 cgacggcgtc gccgaggtcg agttggacat gccggagaac ctcacgacct ggaaggcccg
      241 cgtgtgggtg atgtcggccg gcacgcgcgt cgggcagggc caggcggaga tcgtcacggc
      301 caaggacctg atcgtccgcc tccaggcccc ccgcttcttc gtgcagaagg acgaggtcgt
      361 gctgtcggcc aacgtgcaca actacctcaa gagccccaag cgcgcggagg tcaccctcca
      421 actcgagggc ggctgcctcg agccgctcgt cggggagggc aagaccggcg ggctggtgaa
      481 cgagccgaac ctcgccgcgt cgactctcac gcagaccgtc cacgtcgagc cgggcggtga
      541 gcagcgcgtc gacttccgcg tgcgcgtcgc ctcgcccggc agcgcgaccg tgcgcatgct
      601 cgcgcggacg gacgaggagt ccgacgcgac gcagatgagc ttccccgtgt acgtgcacgg
      661 gatgctcaag actgagagct tcgcgggcgc gatccgcccg agcgcgtcgg cggcgagcgt
      721 gtcgttcacc gtcccgcagg agcgcatccc cgagcagagc cgggtggaga tccgctattc
      781 cccgagcgtg gccggggcga tggtcgacgc gctgccgtac ctcacggatt tcccctacgg
      841 gtgcacggaa caaacgctca accgattcct gccgaccgtc atcacccagc gcgtgctgat
      901 cgccatgggg ctgaacctga aggacatcgc ggccaagcgg acgaacctca acgcccagga
      961 gatcggcgac gaccgcgcgc gggccgcggc tcggcagaac gggacccggc tcaaccccgt
     1021 gttcgacgag gccgtcgtcc tcgccatgac gcgggcgggc gtgacgaagc tcgccaacca
     1081 gcagctcccc gacggcgggt ggggctggtt cagcggctac ggcgaacaaa gcggccccca
     1141 caccacggcc accgtcgtcc acggcctcca gatcgcgcgg gcgaacgacg tcgccctgcc
     1201 gccgggcgtg ctcgagcgcg gcgtcgagtg gctcaagctc tacgaggccc ggcaggtgca
     1261 gatgctgaag aacgccccgg gcaacgtcag cccctcgaag aacgcgtcgg acaacctcga
     1321 cgccttcgtc ttcatggtgc tgtccgacgc ggggcacgtg aacggcgaca tgcgcgagtt
     1381 cctgtaccgc gatcggtcct cgctctcggt ctactgcctc gcgcagctcg gcctggccct
     1441 gcaccggcag aaggggcagg aggagaagct cgcgatggtg ctgcgcaacc tgtcccagta
     1501 cgtcgtccag gacgaagaga accagacggc ttacctcaag ctgccggagt cgggccggta
     1561 ctggtggtac tggtacggct ccgacaccga ggccaacgcg tactacctca agctcctggc
     1621 caagaccgac ggcaagggcg aactccccgc ccggctggcg aagtacctcg tgaacagccg
     1681 ccggaacgcg agctactgga acagcacgcg cgacaccgcg ctgtgcgtcg aagcgctcgc
     1741 cgacttcatc gccgccagcg gcgagggcaa gcccgacatg aaggtggcgg tgcaggtcga
     1801 cgggaaggtg gtgaaggagg tggccatcaa cgccgccaac ctgttcagct tcgacaataa
     1861 ggtcgtgctg gaagggccgg aggtgacgcc gggcgcgcac cggatcgagt tcaccaggac
     1921 cgggaccggc ccgctctact tcaacgcgta cgtgaccaac ttcaccctgg aggagccgat
     1981 caccaaggcg ggcctggaga tccgcgtgca gcggaagtac taccggctc
//
LOCUS       DASQDO010000581         4562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_244338, whole genome shotgun sequence.
ACCESSION   DASQDO010000581 DASQDO010000000
VERSION     DASQDO010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244338"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1112)
                     /locus_tag="VEV43_15910"
     CDS             complement(123..1112)
                     /locus_tag="VEV43_15910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HYP25052.1"
                     /translation="MTQGFRWVALKLLVFTIVTIVVTTYLASIIGNFSLFASPYEITA
                     ELTDASGLLRGDVVKAAGVTVGRVEEIGIRDGIAVVTMSIDEDTELPGELEAKVRFRN
                     LVGQRMITLDAPSPATGRTEPGDVIPIARTEAAFDLSALFNGLRPLIRSTNPEDVNTV
                     TRALTKALSGRADEVEGFLANVGDISTSLASKDQELGVMLGALNEVTADLNGRDAQLR
                     ATLADLDDFLGDLQASRGELAAALVTLDDAATRLGRVVSRNDSHIEAELDDLAVLLDA
                     VEDKREDLRGALRALPEMLVAVERASSYGQWTNIHLVHLCKDDTGECGRRWMP"
     gene            complement(1109..2323)
                     /locus_tag="VEV43_15915"
     CDS             complement(1109..2323)
                     /locus_tag="VEV43_15915"
                     /inference="COORDINATES: protein motif:HMM:NF023315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYP25053.1"
                     /translation="MSDQARRLGAAKKMHVLVGALALVLAAGAVGATWFFLQGGFQTG
                     MPVSAIFSGRGVGQQLPIGGDVKIRGVLVGTIRDVELRDDGNASVELLIEDDMPLPAT
                     TSAEIRSKTVFGQKWVELIPPDAASGPLLAAGDEIPDSRTKEPLELERSLQLGHDLLS
                     RLPLRDLTTVFSTLSEGFTGSEDDARRALDRGIVALRAVNARAGELDLALVQLRQTAQ
                     WLDENDGDILSFMESLDEANRALVDAGPEYRASMQSVPLFLEELAGLQERIEPELGRL
                     VEDGATVAEIVAARSDQLTDIVVQLQAFTTVWNSGLKQPCEGLYESDMTCWQVYQPPG
                     LDSRGLYGDSFGPLSDTASDPLAGVTAGGSDATFSQMLRAYTGRPAPSKLAEVLYQPA
                     REALPELVGRDL"
     gene            complement(2480..3055)
                     /locus_tag="VEV43_15920"
     CDS             complement(2480..3055)
                     /locus_tag="VEV43_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25054.1"
                     /translation="MEPLAVTGREKRQTFLAAIAIALAVFIVGAPVVEAAISRVKLAG
                     GTATAKVKDSNGSTVESEPIADMGLTQAEGSDGAIAVRNFAGGGGLLGAGDCTASTEP
                     AQGPLPNTVTLSGGHIVTGIIITGTGTVRITTQAVGQGQVPLANFVVNANHPNEFVGL
                     GNGLSITTPLTFTGVDGSACNFVVIGQSPGD"
     gene            complement(3145..3831)
                     /gene="trmD"
                     /locus_tag="VEV43_15925"
     CDS             complement(3145..3831)
                     /gene="trmD"
                     /locus_tag="VEV43_15925"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYP25055.1"
                     /translation="MRIDVVTIFPQLFDAPLGTSLLGRAVAGGLLDVRVHDLREHGLG
                     KHRSVDDEPYGGGAGMVMRPEPLFAAVEEAKGPDSHVVLLSPRGRKLDHAVVAELARR
                     DHLVLVCGRFEGVDERVAEHLVDEELSIGDYVLAGGELPALVVIEAVSRLMPGVLGNA
                     ESLASESHAEGLLEYPQYTRPAEFRGWRVPDVLLSGDHGAVDRWRREQSELITRERRP
                     DLGGARSPST"
     gene            complement(3828..4331)
                     /gene="rimM"
                     /locus_tag="VEV43_15930"
     CDS             complement(3828..4331)
                     /gene="rimM"
                     /locus_tag="VEV43_15930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HYP25056.1"
                     /translation="MTPSPELLLAGQIGKPHGLGGEVYVIRISDDPRRFEPGSELLHE
                     DGRELVVEAARDHRNRFLVKFEGVDDRDAAESLRGPLYVPSGEARPLEAGEFWEHELQ
                     GLDVVHARTGDKVGTVAHVLDGPAQDLLVLETPVGERMVPLVAEIVVNVDVDGGRVEI
                     DPPEGLL"
     gene            complement(4328..>4562)
                     /locus_tag="VEV43_15935"
     CDS             complement(4328..>4562)
                     /locus_tag="VEV43_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HYP25057.1"
                     /translation="SREFLEYVTPWLVDHPDEVMITEGDGERDEVVYELSVHPEDMGK
                     IIGKRGRIIRSLRALARAAGGRDGRAVSVEVVD"
BASE COUNT          673 a         1624 c         1560 g          705 t
ORIGIN      
        1 cagcgtgtac acgccgcgca gtccctggaa gcggttgatc gagaaggcgg cggtcacccc
       61 ggccgtgatc agcacgatgc aggtgatgcc gaccagccag gccctgcgct cccggaacga
      121 cctcacggca tccacctccg gccacactcg ccggtgtcgt ccttgcacag gtggacgagg
      181 tggatgttag tccactggcc gtacgagctc gcgcgctcga ccgcgacgag catctcaggc
      241 agcgcgcgca gagcgccgcg caggtcctct cgcttgtcct cgaccgcgtc cagcagcacg
      301 gcgaggtcgt cgagctcggc ttcgatgtgc gagtcgttgc gcgacacgac gcgtccgagc
      361 cgggtcgccg cgtcgtccag cgtcacgagg gccgcggcca gctcgccgcg gctcgcctgc
      421 aggtcgccga ggaagtcgtc gaggtcggcg agcgtcgcgc gcagctgcgc gtcgcgcccg
      481 ttgaggtccg cggtcacctc gttaagcgca ccgagcatca cgccgagctc ctggtccttc
      541 gaggcgaggc tcgtcgagat gtcgccgacg ttggcgagga agccctcgac ctcgtcggcc
      601 ctccccgaca gagccttcgt gagcgcccgc gtcacagtgt tgacgtcctc ggggttcgtg
      661 gaccggatca gcggacgcag cccgttgaac agcgccgaca ggtcgaacgc cgcctcggtc
      721 cgcgcgatcg ggattacgtc acccggctcc gtgcgccccg tcgccggcga cggcgcgtcc
      781 agcgtgatca tccgctggcc gacgaggttc ctgaacctga ccttggcctc gagctcgccg
      841 ggaagctccg tgtcctcgtc gatcgacatc gtcaccacgg cgatcccgtc gcggatgccg
      901 atctcctcga cacgcccgac ggtcaccccc gcagccttca cgacgtcgcc gcgcagcagc
      961 ccgctcgcgt ccgtgagctc cgcggtgatc tcgtacgggc tggcgaacag cgagaagttg
     1021 ccgatgatgc tcgcgaggta cgtcgtgacc acgatcgtca cgatcgtgaa gacgagcagc
     1081 ttgagcgcga cccagcggaa gccctgcgtc acaggtccct cccgacgagc tcggggagag
     1141 cctcacgcgc cggttggtac aggacctcgg cgagcttgga gggggccggc cggcccgtgt
     1201 acgcgcggag catctgcgag aacgtcgcgt cggacccgcc cgcggtcaca ccggcaagcg
     1261 ggtcgctcgc cgtgtcgctc agggggccga acgagtcgcc gtagaggccg cgtgagtcga
     1321 ggcccggagg ttggtagacc tgccagcacg tcatgtcgct ctcgtagagg ccctcgcacg
     1381 gctgcttcag ccccgagttc cacacggtcg tgaaggcctg cagctgcacg acgatgtccg
     1441 tgagctgatc ggaccgcgcg gcgacgatct cggcgaccgt ggcgccgtcc tcgacgagac
     1501 gcccgagctc cggctcgatc ctctcctgca ggcccgcgag ctcttcgagg aacagcggca
     1561 ccgactgcat cgacgcccgg tactccggcc ccgcgtcgac gagggcacgg ttcgcctcgt
     1621 cgagcgactc catgaacgag aggatgtcgc cgtcgttctc gtccagccat tgggccgtct
     1681 gccgtagctg gaccagcgcg aggtccagct cgccggcgcg cgcgttgacg gcgcggagcg
     1741 cgacgatccc gcggtcgagg gcgcggcggg cgtcgtcctc gctgcccgtg aatccttccg
     1801 agagcgtaga gaagacggtg gtgaggtcgc gcaacgggag ccgcgacagg aggtcgtggc
     1861 ccagctgcag cgagcgctcg agctcgaggg gctccttcgt cctggagtcc gggatctcgt
     1921 ctcccgcggc gagcagcggt ccgctcgcgg cgtccggcgg gatcagctcg acccacttct
     1981 ggccgaacac ggtcttggaa cggatctcgg cggacgtcgt cgccggcagc ggcatgtcgt
     2041 cctcgatgag gagctcgacc gacgcgttgc cgtcgtcacg gagctcgacg tcgcggatcg
     2101 tcccgacgag gacgccgcgg atcttgacgt cgccgccgat cgggagctgc tgccccacgc
     2161 cgcggccgga gaagatcgcg ctgacgggca tgcccgtctg gaacccgccc tggaggaaga
     2221 accacgtcgc gccgaccgcc cccgcggcga ggacgagcgc gagcgcgccc acgaggacgt
     2281 gcatcttctt ggctgcaccg aggcgtcgtg cctggtcgct catggctccc ttgctgccgg
     2341 tggtgaaatg cccgctttgc gctacctatc ttcgggccgc ggccccctcg gggaccatcg
     2401 gtaaaactgc gtagcccgag cgcggaagag gcgccccgaa gggcgcctcc ctcgctctgt
     2461 gacggttact tcctagctct cagtcgccgg ggctctggcc gatgaccacg aagttgcagg
     2521 ccgagccgtc gacgcccgtg aacgtgagcg gcgtcgtgat gctcaggccg ttgccgaggc
     2581 cgacgaactc gttcgggtga ttggcgttca cgacgaagtt cgcgagcggg acctgtccct
     2641 gcccgaccgc ctgcgtcgtg atgcggaccg ttccggtgcc cgtgatgatg atgccggtca
     2701 cgatgtgacc gcccgagagg gtcacggtgt tcgggagcgg gccctgtgcg ggctcggtcg
     2761 aagccgtgca gtcaccggcg cccagcaggc cgccgccgcc ggcgaagttg cggacggcga
     2821 tcgcgccgtc gctgccctcg gcctgcgtca ggcccatgtc ggcgatcggc tcggactcga
     2881 ccgtcgatcc gttcgagtcc ttcaccttcg cggtggcggt tccgcccgcg agcttcactc
     2941 ggctgatcgc cgcttcgacg acgggcgcac ctacgatgaa taccgcgagc gcgatcgcga
     3001 tggcggcgag gaacgtctgg cgcttctcgc gccccgttac ggcaagtggc tccatgaatc
     3061 ctcctcgttg agtgacaacg tcttgacgac aaacttaggg aacccgcgct gttatggcga
     3121 cggttcccga agaacttgcc tgatctacgt agaaggagaa cgagcgccgc cgaggtccgg
     3181 ccgccgctcg cgcgttatca gctcggattg ctctcggcgc caccggtcca ctgcaccgtg
     3241 gtcaccggag agaagcacgt cggggacgcg ccagccgcgg aactccgcgg ggcgcgtgta
     3301 ctgcggatac tcgagcagcc cctctgcgtg ggactccgac gccagcgact cggcgttgcc
     3361 gagcacgccg ggcatgagac gtgagaccgc ttcgatgacg acgagggccg ggagctcgcc
     3421 gccggccagg acgtagtcac cgatcgacag ctcttcgtcg acgaggtgct cggcgactct
     3481 ctcgtcgaca ccctcgaagc gcccgcacac gagcacgagg tggtcacgcc gagcgagctc
     3541 cgccacgacc gcgtggtcga gcttgcggcc gcgcggcgag agcaggacca cgtgcgagtc
     3601 cggccccttg gcctcctcga ccgcggcgaa caacggctcc ggcctcatga ccatgccggc
     3661 ccccccgccg tacggctcgt cgtcgaccga acggtgcttg ccgagcccgt gctccctgag
     3721 gtcgtggacc cggacgtcga ggagccctcc ggcgaccgcc ctgccgagca ggctcgtccc
     3781 cagcggggcg tcgaacagct gcgggaagat cgtgacgacg tcgatcctca cagcaggcct
     3841 tccggcgggt cgatctcgac ccggcccccg tccacgtcga cgttcacgac gatctcggcg
     3901 acgagcggga ccatccgctc gcccaccggc gtctcgagga ccagcaggtc ttgcgcaggg
     3961 ccgtccagca cgtgcgcgac cgtcccgacc ttgtcgcccg tcctcgcatg cacgacgtcg
     4021 aggccctgga gctcgtgttc ccagaactcg ccggcttcga gtgggcgcgc ttcaccggac
     4081 gggacgtaga gggggccgcg cagagactcc gcggcgtcgc ggtcgtcgac gccctcgaac
     4141 ttgacgagga agcggttgcg gtggtcgcgg gcggcctcga cgacgagctc gcgcccgtcc
     4201 tcgtgcagca gctccgaccc cggctcgaac cggcgcgggt cgtcggagat ccggatgacg
     4261 tagacctcgc cgcccaggcc gtgcggcttc ccgatctgcc ccgcgagcag cagctcgggg
     4321 gaaggggtca gtccacgacc tccacggaga cggcacgtcc gtcgcggccc ccggccgcgc
     4381 gggccagcgc acgcagcgac ctgatgatgc ggccgcgctt gccgatgatc ttgcccatgt
     4441 cctcggggtg gaccgacagc tcgtacacga cctcgtcgcg ctcgccgtcg ccctctgtga
     4501 tcatgacctc gtcgggatgg tcgacgagcc acggcgttac gtactcgagg aactctctgc
     4561 tc
//
LOCUS       DASQDO010000582         3319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_917241, whole genome shotgun sequence.
ACCESSION   DASQDO010000582 DASQDO010000000
VERSION     DASQDO010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917241"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1528)
                     /locus_tag="VEV43_15940"
     CDS             complement(38..1528)
                     /locus_tag="VEV43_15940"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HYP25058.1"
                     /translation="MSRRSRIARARAAGLALCIALLGLPAAAAGQPISHHPAPPTSAT
                     LRKAVKTRNVLKHLRKLQDIAFAHGGTRAAGTDGYEASADYVARKLKRYGWKVYLQSF
                     EFLAFTEVEPTVMEQVAPDEIPYENGIDFSIMSYSGSGDVTAPVTPVDVNVADPSEIT
                     SGCEDADFAGFPAGNIALIQRGTCTFGEKAVNAQEAGASAVIIFNQGNTEERSLLLEG
                     DLGDDTTATIPAVGTTFALGEELVAAAANGLRIHLVTVTDAELLTTRNVFGEWRKSGD
                     PSAVIMAGAHLDSVPEGPGINDNGTGVAALLETARQIPRAPYFFEDRIRLAFWGAEEA
                     GLIGSTEYVASLSEEEAAAITGYLNFDMVGSPNYARMIYDGNGSAFPDVGAGPEGSET
                     IERVFQRYFQRQGLPTTQTPFEGRSDYGPFIDIGIPAGGLFTGAEDVKTAEEAELFGG
                     TAGEAYDLCYHEVCDTLSNVNRRVLGQMSDAIAHAIFRFATGADSL"
     gene            1720..2769
                     /locus_tag="VEV43_15945"
     CDS             1720..2769
                     /locus_tag="VEV43_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25059.1"
                     /translation="MNDDRDRTEGPKRHRLAWSAALAVVLTAVCAPLAGAAGVQLPRN
                     PSLPQVPQVPLPVEEPPLPELPDIPVVPDVTTVPVPVEELEGATDPIVDEATGAVDDV
                     VDEVTEPGGPVGDVVEEVVDPGSPVGGVVDEVLDPDGPVGGVVDEVTDPETPVGGVVD
                     EVLDPEGPVGGVVDEVTDPDGPVGGVLDDVKGKVGGVVDGVGGVVDGVVGPLPEVPNL
                     PGAGEPGALDPDAGTGVGIGGGGSFGRTPSEMAAYRPATAAATPVDGFAASVLSARQT
                     PPVAAPGARDLPAPSLVERIARSASDVAQKVAFPLLLAALVGAFVVFQNRMDRKDPKL
                     ALAAIDADEDLLGFE"
     gene            2766..>3319
                     /locus_tag="VEV43_15950"
     CDS             2766..>3319
                     /locus_tag="VEV43_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25060.1"
                     /translation="MSNGRSAGEMVSLGERMGYLQALRATLALVVVVSAIVAHDVVGA
                     AIEDLLPVTVGYLVLSATGEGLRRAGRMRGLMTVGGMLMVDGLFLAWVLYITGGTQSP
                     LRFLVYVHLIAVTLLASYRTGLKIALWHSLLFFVVFYAQVSGIVPQIDSAAEATTRSR
                     PSAFNVVAFWVVALATTAFSSVNER"
BASE COUNT          436 a         1141 c         1199 g          543 t
ORIGIN      
        1 ggacattccc ggaaccgccc ccttcttgtg tcacggctta gagcgagtcg gctccggtgg
       61 cgaagcggaa gatcgcgtgc gcgatcgcgt cggacatctg cccgagcacc ctgcggttga
      121 cgttcgacag cgtgtcgcag acctcgtggt agcagaggtc gtatgcctcg cctgcggtcc
      181 ccccgaacag ctcggcctcc tcggcggtct tgacgtcctc cgcgccggtg aacaggcccc
      241 cggccgggat gccgatgtcg atgaacgggc cgtagtcgga gcgcccttcg aacggggtct
      301 gcgtcgtggg gaggccctgc ctctggaagt aacgctggaa cacgcgctcg atcgtctcgg
      361 agccctcggg gcccgcgccg acgtcgggga aggccgagcc gttcccgtcg tagatcatcc
      421 gcgcgtagtt cggcgagccg accatgtcga agttgaggta gccggtgatc gcggcggcct
      481 cttcctcgct gagcgacgct acgtactcgg tcgaaccgat aaggccggcc tcctcggcgc
      541 cccagaacgc gaggcggatg cggtcctcga agaagtacgg agcgcgcggg atctggcgcg
      601 cggtctcgag gagcgccgca acccccgtcc cgttgtcgtt gatgccgggg ccttcgggga
      661 cgctgtcgag gtgcgcgccg gccatgatca ccgcgctcgg gtcgccgctc ttgcgccact
      721 cgccgaagac gttcctcgtc gtgagcagct cggcgtcggt gacggtcacg aggtggatcc
      781 gcaggccgtt ggcggcggcc gcgacgagct cctcgccgag agcgaacgtc gtgccgacgg
      841 ccgggatcgt cgcggtcgtg tcgtcgccga ggtcgccctc gagcagcagg ctgcgctcct
      901 cggtgttgcc ctggttgaag atgatcaccg cggaggcgcc ggcctcctgg gcgttgacgg
      961 ccttctcgcc gaacgtgcac gtgccacgct ggatcagcgc gatgttgccc gccgggaacc
     1021 cggcgaagtc cgcgtcctcg caaccgctcg tgatctcgct cgggtccgcg acgttcacgt
     1081 cgaccggcgt caccggagcg gtcacgtcac cgctgcccga gtacgacatg atgctgaagt
     1141 cgatcccgtt ctcgtagggg atctcgtccg gagcgacctg ctccatgacg gtcggctcga
     1201 cctcggtgaa cgcgaggaac tcgaacgact gcaggtagac cttccagccg tagcgcttca
     1261 gcttgcgcgc gacgtagtcc gcggaggctt cgtagccgtc ggtccccgcg gcccgcgtgc
     1321 ccccgtgggc gaacgcgatg tcctggagct tgcggaggtg cttcaggacg ttgcgcgtct
     1381 tgacggcctt gcggagcgtc gccgacgtcg ggggtgccgg atggtgcgag atcggctgcc
     1441 cggcggccgc ggccggaagc cccaagagcg cgatgcacaa cgccagacct gcagcgcgtg
     1501 cccgcgcgat cctgctccgt cgagacattc cggacctcct ccgttcgtgg cgccgccggc
     1561 gccgtcgcaa cccgtccgaa ctatagaggc ggcgggtggg gccctcgccc tccgggccgg
     1621 gcgaatcggg acgagccggg cacttcccgt acgtagttaa agcaggtcgg gaggctgccg
     1681 atacgtagat atcgctgccg tgaccaaggg agacccgact tgaacgacga ccgcgaccgc
     1741 accgagggcc cgaagcgtca ccggctcgcg tggagcgctg ctctcgcagt cgtgctgact
     1801 gcggtgtgcg ccccgctggc gggtgccgcc ggggtgcagc tccctcggaa cccgtcgctg
     1861 ccgcaggtgc cccaggtgcc cctcccggtc gaagagccgc cgctccccga gctcccggac
     1921 atccccgtcg tgccggacgt gacgacggtc ccggttcccg tcgaggagct cgagggtgcg
     1981 accgatccga tcgtcgacga ggcgacgggt gcggtcgatg acgtcgtcga cgaagtgacg
     2041 gagcccggcg gaccggtcgg cgacgtggtg gaagaggtgg tcgaccccgg gagtcccgtc
     2101 ggcggagtcg tggacgaggt cctcgatccc gacggaccgg tcggcggtgt ggtcgacgag
     2161 gtcacggacc cggagacccc tgtcggtgga gtcgtggacg aggtcctcga tcccgaaggg
     2221 cccgtcggcg gagtcgtcga cgaggtcacg gatccggacg gccccgtcgg cggcgtcctc
     2281 gacgacgtga agggcaaggt cggcggagtc gtcgacggtg tcggcggcgt ggtcgacggt
     2341 gtcgtcggcc ccctgcccga ggtcccgaac ctccccggtg ccggcgagcc cggcgcgctc
     2401 gacccggacg ccggtacggg cgtcgggatc ggcgggggtg gctcgttcgg ccgcacgccc
     2461 tcggagatgg ccgcttaccg cccggccacc gcggcggcga ccccggtcga cgggttcgct
     2521 gcgagcgtcc tttcggcacg ccagacgcca ccggttgcgg cccccggggc gcgtgacctc
     2581 ccggccccct cactcgtgga gcgcatcgca cgttccgctt ccgacgtcgc tcagaaggtc
     2641 gctttccccc tgctgctcgc ggcgctggtc ggggcgttcg tcgtcttcca gaaccggatg
     2701 gaccgcaagg acccgaagct cgcgctcgcg gcgatcgacg ccgacgaaga cctgctgggc
     2761 ttcgaatgag caacggtcgc tccgccggcg agatggtctc gctcggcgag cggatgggat
     2821 acctgcaggc gctgcgggcc acgctcgcgc tcgtagtcgt cgtgtcggcg atcgtggcgc
     2881 acgacgtcgt gggcgcggcg atcgaggacc tgctgccggt gacggtcgga tacctcgtgc
     2941 tgtccgcgac cggcgagggc ctgcggcgcg cgggtcgcat gcgcggcctc atgaccgtcg
     3001 gcgggatgct gatggtcgac gggctcttcc tcgcgtgggt cctgtacatc acgggcggca
     3061 cgcagagccc gctgcgcttc ctggtctacg tgcacctcat cgcggtgacg cttctcgcgt
     3121 cgtaccggac cggcctgaag atcgcgctgt ggcactcgct tctgttcttc gtcgtgttct
     3181 acgcgcaggt gtcggggatc gtcccgcaga tcgactcggc ggccgaggcg acgacgcgca
     3241 gccggccgtc ggccttcaac gtcgtcgcgt tctgggtcgt cgcgctcgcg acgactgcgt
     3301 tctcctcggt caacgagcg
//
LOCUS       DASQDO010000583         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_488883, whole genome shotgun sequence.
ACCESSION   DASQDO010000583 DASQDO010000000
VERSION     DASQDO010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488883"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VEV43_15955"
     CDS             <1..803
                     /locus_tag="VEV43_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25061.1"
                     /translation="EGVPYISAGVTEQGVNTLSTYFAIWASYKQQAPLLVDYMTNKLG
                     AKGERNGMVYFNTPGFLDGRAAWEDAMSGAGASVDYMRAVPKNAGQTDAATVATDLCQ
                     QQIKNVNVLTSPTFFIQLANEAERQCDIQWTGVGLTMTIDTVASVACRNRSSINNAKF
                     LSPFPAFVDSNRFDPNFRKAGGTDDIMFGLWGMSKILHQMLLQPGRNLTRERFMWHVQ
                     QMGKISTGVLPDVQFSPTDHFGGSEMHLNRADCSRNVWTTEIPFFGGF"
     gene            858..1871
                     /locus_tag="VEV43_15960"
     CDS             858..1871
                     /locus_tag="VEV43_15960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYP25062.1"
                     /translation="MRTGIVTGVIVAVIAALLIASQGDLTGPGAFVILGLTNGAIFGL
                     VALGLVLVYKGTRVFNFAQGEFGTCAAWFVLVAMGESRFGGPKLPYWAAVIVALVGAL
                     LLGLLFERVVARPLLNAPRVTVLVATIATALLLVGLQIVFFEIDQESLRGIFTGDDAG
                     IFSTKKFFNLNIDPQRQVAIAVLLAIGVVLAYFFSRTDLGLAVLATSQDAFATRVVGI
                     GVERMSRFIWGGAALLGAIAAVIYVPVAGLLVPGVMTSNVLIPSFTGAVLGGMNSLPG
                     AFLGGITIGVVQTVATWAVGHYHLGEKAIVEVVPASEQISILIVLLVVLLARPAGLLG
                     TEA"
     gene            1868..>3034
                     /locus_tag="VEV43_15965"
     CDS             1868..>3034
                     /locus_tag="VEV43_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYP25063.1"
                     /translation="MSASSEAATLTQPVVEDDKADEVWRPDARGWAARLGVLALIFGG
                     LAFLLATVTQTWANLIAGAAIYAIIGLSLNVVLGYTGQVSLGHHGFVGLSAFVSAGLV
                     SEQGQTFWVGLGAAVLTGAATAGLLGLVALRIKGLYLALITLTFGYVAANSIFEIPAL
                     TKGGQGMPAPRPEGFTSNRGYALLCILMLAVALFVDWRIVRSKVGRAILAVKQSEDVA
                     ASYAVNVTFYKAFAFILSGAFAGLAGSLFAHRVGVVVANNFRFEAALLWVLMVVVGGL
                     GRRTGVVIASAFFFLFPSLIGEIEFLRHFIEDVLNREPDYFTLVIGSLLALEVIIFAP
                     GGLGGRLSPITTWLGGARFSMHPEGGGGHRKKEKSGPGRREKLMKRLGLERGTPP"
BASE COUNT          436 a         1052 c         1007 g          539 t
ORIGIN      
        1 ccgagggcgt cccgtacatc tctgccggcg tgaccgagca gggcgtcaac acgctctcca
       61 cgtacttcgc catctgggcg tcgtacaagc agcaggcgcc gctcctcgtc gactacatga
      121 cgaacaagct cggcgccaag ggcgagcgca acgggatggt gtacttcaac acgccgggct
      181 tcctcgacgg acgtgccgcg tgggaagacg cgatgtccgg cgccggcgcc tcggtcgact
      241 acatgcgggc ggtcccgaag aacgccggac agaccgacgc cgccaccgtc gcgaccgacc
      301 tgtgccagca acagatcaag aacgtgaacg tgctcacctc gccgacgttc ttcatccagc
      361 tggcgaacga ggccgagcgg cagtgcgaca tccagtggac cggcgtcggg ctcacgatga
      421 cgatcgacac ggtcgccagc gtggcctgcc ggaacaggtc gtcgatcaac aacgccaagt
      481 tcctgagccc gttcccggcc ttcgtcgact cgaaccggtt cgacccgaac ttccgcaagg
      541 ccggcgggac cgacgacatc atgttcggcc tctggggcat gagcaagatc ctgcaccaga
      601 tgctcctcca gcccggcagg aacctcacgc gagagcgctt catgtggcac gtccagcaga
      661 tgggaaagat ctcgaccggc gtcctgcccg acgtccagtt ctcgccgacg gaccacttcg
      721 gcggcagcga gatgcacctg aaccgggcag attgctcgcg caacgtctgg acgaccgaga
      781 tcccgttctt cggcggcttc tagaacgtcg ccgcgaagta gccccgcccg cgccgagacg
      841 ataggttctc tcccgccatg aggacgggga tagtcaccgg tgtcatcgtc gcggtgatcg
      901 cggccctctt gatcgcgtcc caaggcgacc tcaccggccc cggtgccttc gtcatcctcg
      961 ggctcacgaa cggcgcgatc ttcggcctcg tcgccctcgg cctcgtcctc gtctacaagg
     1021 ggacccgcgt cttcaacttc gcacagggcg agttcgggac gtgcgcggcg tggttcgtcc
     1081 tcgtcgccat gggcgagtcc cgcttcggcg gcccgaagct cccctactgg gctgcggtca
     1141 tcgtggcgct cgtcggggcc ttgctgctgg ggcttctctt cgagcgcgtg gtggctcgcc
     1201 ccctcctcaa cgctccgcgg gtcaccgtcc tcgtcgccac catcgcgacc gcgctcctcc
     1261 tggtcgggct gcagatcgtc ttcttcgaga tcgaccagga atccttgagg gggatcttca
     1321 ccggcgacga cgcggggatc ttcagcacca agaagttctt caacctgaac atcgacccgc
     1381 aacggcaggt cgcgatcgca gtgctgctcg ccatcggcgt ggtccttgcg tacttcttct
     1441 cccggaccga tctcggcctg gccgtcctcg cgacgtccca ggatgcgttc gccacgaggg
     1501 tggtagggat cggcgtcgag cgcatgtcgc gcttcatctg gggcggcgcc gcgttgctcg
     1561 gcgcgatcgc agcggtgatc tacgtgccgg ttgcggggtt gctcgtgccg ggggtgatga
     1621 ccagcaacgt cctgatcccg tcgttcacag gcgcggtcct cggaggcatg aacagccttc
     1681 cgggcgcgtt cctcggcggg ataacgatcg gcgtcgtgca gacggtcgcg acctgggccg
     1741 tcgggcacta ccacctgggc gagaaggcga tcgtcgaggt cgtgcccgcg tcggagcaga
     1801 tctcgatcct gatcgtgctc ctcgtcgtcc tgctcgcgcg gcccgcgggc ctgctgggga
     1861 ccgaggcatg agcgcgtcgt cggaggccgc gacgctcacg cagccggtcg tcgaggacga
     1921 caaggcggac gaggtctggc ggcccgacgc ccgcggctgg gccgcgcgcc tcggcgtcct
     1981 cgccctgatc ttcggcggcc tcgccttcct gctcgcgacc gtgacccaga cctgggcgaa
     2041 cctgatcgcg ggggcggcta tctacgcgat catcggcctc tccctgaacg tggtcctcgg
     2101 ctacaccggc caggtctcgc tcggccatca cggcttcgtc ggcctctcgg cgttcgtctc
     2161 ggccgggctc gtcagcgagc aggggcagac gttctgggtg ggtctcggag ccgcggtcct
     2221 cacgggagcg gcgacggcgg ggctgctcgg cctcgtggcg ctgcggatca agggcctcta
     2281 cctcgcgctg atcactctga cgttcggcta cgtcgcggcg aactccatct tcgagatccc
     2341 ggccctcacg aagggcgggc agggcatgcc ggcgccgcgg ccggagggct tcacgagcaa
     2401 ccgcggctac gcgctgctct gcatcctcat gcttgcggtc gcgctcttcg tcgactggag
     2461 gatcgtccgc tcgaaggtcg gccgcgcgat cctcgcggtc aagcagtccg aagacgtggc
     2521 cgcctcgtac gcggtcaacg tcaccttcta caaggccttc gcgttcatcc tgtcgggggc
     2581 gttcgcaggg ctcgccggca gcctgttcgc gcaccgcgtg ggcgtcgtcg tggcgaacaa
     2641 cttcaggttc gaggccgcgc tcttgtgggt gctgatggtc gtggtcggcg ggttgggccg
     2701 gaggaccggc gtcgtcatcg cgtcggcgtt cttcttcctg ttcccgtcac tgatcggcga
     2761 gatcgagttc ctgaggcact tcatcgagga cgtcctcaac cgcgagcccg actacttcac
     2821 gctcgtcatc ggctccctgc tcgctctcga ggtcatcatc ttcgcccccg gagggctggg
     2881 aggccgtctc tccccgatca cgacgtggct cggcggcgcg cgcttctcga tgcatcccga
     2941 gggcggcggc ggccatcgca agaaggagaa gagcgggccg gggcgccgcg agaagctgat
     3001 gaagcggctg ggtctcgagc gagggacgcc cccc
//
LOCUS       DASQDO010000584         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_734164, whole genome shotgun sequence.
ACCESSION   DASQDO010000584 DASQDO010000000
VERSION     DASQDO010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734164"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VEV43_15970"
     CDS             complement(<1..772)
                     /locus_tag="VEV43_15970"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYP25064.1"
                     /translation="MSRWATVEFPLDLPVEKKGDHWVARAGDRELKLTNLDKVYWPEE
                     GYTKGDLLAYYFNVSPTMLPHLEDRPLTLKRMPHGVGGDFFYEKNAPSHRPSWMPTLP
                     VPSDGGTKIINYLTVCDPVHMLWVTNLGAIEFHPLHGRGADQRFPTYAFFDLDPMHGA
                     GWEEVRHVARLVKVVLDQLGITAYPKTSGATGMQIMVPLDGTVDYDHVRGFVGTVSDL
                     VHAADPDTTTIEWEVSKRSDKVFLDVNMNREGANIAAAY"
     gene            821..1339
                     /locus_tag="VEV43_15975"
     CDS             821..1339
                     /locus_tag="VEV43_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HYP25065.1"
                     /translation="MAKIGFILDEMFEDSEFQVPYERVKEAGHEAVVIGLEAGKKLEG
                     KKGDVTTTTEVAAGDVDAGELAALVIPGGYSPDKIRTDEAMVGLTKSVFGAGKPVAAI
                     CHAGWMLAEADVVRGKTVTSWPSIKTDLVNAGAQWVDQEVVEDGNLITSRMPDDLEAF
                     SKALLVQVEQQA"
     gene            complement(1398..>1721)
                     /locus_tag="VEV43_15980"
     CDS             complement(1398..>1721)
                     /locus_tag="VEV43_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HYP25066.1"
                     /translation="LVTADATGGSANFARSLGPFVASMFYETGDIPWGDLLVYGLGPL
                     IGGMAAALVYESITGLETEAPAPQPGAATSPGDEIIGDNGLIDEDPGSTYGGPGDPPP
                     PDRTF"
BASE COUNT          282 a          572 c          591 g          276 t
ORIGIN      
        1 agtacgcggc cgcgatgttc gccccttccc ggttcatgtt cacgtccagg aagaccttgt
       61 cggagcgctt gctgacctcc cactcgatcg tcgtcgtgtc ggggtccgcc gcgtgcacga
      121 ggtccgagac cgtgccgacg aacccgcgca cgtggtcgta gtccacagtg ccgtcgagcg
      181 ggaccatgat ctgcatcccg gtcgcgcccg aggtcttcgg gtacgcggtg atcccgagct
      241 ggtcgaggac gaccttcacg agccgcgcga cgtgacgcac ctcctcccag cccgctccgt
      301 gcatcgggtc gaggtcgaag aacgcgtacg tgggaaagcg ctggtccgcg ccccgtccgt
      361 ggagcgggtg gaactcgatc gcaccgaggt tcgtcaccca cagcatgtgc acggggtcgc
      421 agaccgtgag gtagttgatg atcttcgtcc cgccgtccga cggcaccggc agcgtcggca
      481 tccacgaggg ccggtgcgac ggcgcgttct tctcgtagaa gaagtcgccg ccgacgccat
      541 ggggcattcg cttcaacgtc aacggccggt cctcgaggtg ggggagcatc gtcggcgaga
      601 cgttgaagta gtaggcgagc aggtcgccct tcgtgtagcc ctcctcgggc cagtacacct
      661 tgtcgaggtt cgtgagcttg agctcgcggt cgcccgcgcg cgccacccag tggtcgccct
      721 tcttctcgac ggggaggtcg agcgggaact cgaccgttgc ccaacggctc atgcggggta
      781 gataccacgc aagacgaaaa gcccgagcaa ggaggccgca gtggcgaaga tcggtttcat
      841 cctcgacgag atgttcgaag actccgagtt ccaggtcccg tacgagcgcg tgaaggaggc
      901 cgggcacgag gcggtcgtga tcgggctcga agccggcaag aagctcgagg gcaagaaggg
      961 cgacgtcacg accacgaccg aggtcgcggc cggcgacgtc gacgcgggcg agctcgcggc
     1021 cctcgtgatc cccggcggct actcgccgga caagatccgc accgacgagg cgatggtcgg
     1081 cctgaccaag tcggtcttcg gcgccggcaa gcccgtcgcc gcgatctgcc acgcgggctg
     1141 gatgctcgcg gaggccgacg tcgtgcgcgg caagaccgtc acgtcgtggc cgtcgatcaa
     1201 gaccgacctc gtcaacgccg gcgcgcagtg ggtcgatcag gaggtcgtcg aagacggcaa
     1261 cctcatcacc tcgcgcatgc cggacgacct cgaggcgttc tcgaaggctc tgctcgtcca
     1321 ggtcgagcag caggcgtagt tccgcggaac gaaagaagcc ccctccgcga acggaggggg
     1381 ctttcctttc gatcgggtca gaaggtccgg tccggtggcg gtgggtcccc cggccccccg
     1441 tacgtcgagc ccgggtcttc gtcgatcaat ccgttgtcgc cgatgatctc gtcgcccggc
     1501 gacgtcgcgg cgcccggttg cggcgccggt gcctctgtct cgaggccggt gatgctctcg
     1561 tagacgagcg cggccgccat ccctccgatc aagggaccta gtccgtagac caggaggtca
     1621 ccccacggga tgtcacccgt ctcgtagaac atcgaggcca cgaaggggcc gaggctccgc
     1681 gcgaagttcg ccgagccacc ggtcgcgtcc gcggtcacga g
//
LOCUS       DASQDO010000585         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_856381, whole genome shotgun sequence.
ACCESSION   DASQDO010000585 DASQDO010000000
VERSION     DASQDO010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856381"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..788)
                     /locus_tag="VEV43_15985"
     CDS             complement(243..788)
                     /locus_tag="VEV43_15985"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HYP25067.1"
                     /translation="MTPVQEVMPGLVAESPSDGGPAALLDGARREAMARAGRLLATRA
                     RSEAELTRRLAEAGFEASVVSAVIDRLRTLGLVDDRAFAVQWVRERSTRKKLSSRALL
                     HELTGKGIDREVAEAALAEGGLDETAQATDLASRYVRRVASKPLREQATKIQQMLSRR
                     GYSYEVCDAAVKAVLPPEGWD"
     gene            complement(789..1898)
                     /gene="recA"
                     /locus_tag="VEV43_15990"
     CDS             complement(789..1898)
                     /gene="recA"
                     /locus_tag="VEV43_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005513719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HYP25068.1"
                     /translation="MRESRAAVVDTSRDAKGRDAALDMALAQIETPHGKGSIMRLGDA
                     GIASGIEAIPTGSLALDLALGIGGLPRGRIVEIYGPEGSGKTTVSLHVIAEAQKNGGL
                     VSFIDAEHALDPSYAKALGVDIDNLLCSQPDTGEQALEIADTLVRSGAMDVIVVDSVA
                     ALVPRAEIEGEMGDSHVGLQARRMSQALRKLAGSVNRSKTILIFINQIREKIGVMFGS
                     NETTPGGRALKFYSSVRMDVRRIDSLKDGAEVVGSRVRVKVVKNKVSAPFKKAEFDIQ
                     YGYGISKEGSLLDVAIDHGIVRKSGAWFIYGDDQLGQGRENAKSFLAGNPDIATEIEL
                     KIKDKLGLLGTDEDDDAEGGLGRPPAQKPVPEAAS"
     gene            complement(2176..2577)
                     /locus_tag="VEV43_15995"
     CDS             complement(2176..2577)
                     /locus_tag="VEV43_15995"
                     /EC_number="3.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF020622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A2"
                     /protein_id="HYP25069.1"
                     /translation="MKSGSKRKLVRSLVVAASLASSMTVGGSTPAAAHIQTPTDYCSY
                     APDYVPGLYDFRHACAHHDYAYRTHWVSRATADTYFRWAMESECRNRHPWYSSNLGKC
                     LAVKEVYYFFVRRFGQSAYDRWETTAAMGPR"
     gene            complement(2773..3195)
                     /locus_tag="VEV43_16000"
     CDS             complement(2773..3195)
                     /locus_tag="VEV43_16000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYP25070.1"
                     /translation="MAQGSETARVNAIWDRVARVYDKRISFFERRLFQGGREWVCSRA
                     RGKTLEIAVGTGRNLGLYGDGVELTGIELSEEMLARARDRAVRLGVGADLRRGDAQSL
                     DHVTREPLDHLRREGFAIDELERLKWGIVDRVSATKPS"
BASE COUNT          460 a         1132 c         1060 g          546 t
ORIGIN      
        1 tccgcccggt tccgtagcgt cgaggggacg agaggggggg cttcgatgag cggatccagt
       61 tgggccacgg gcaggtcgag ggcgcagcgg cgccgggtgc ggattcagat ccgccgcggg
      121 ggccgcggct aggcaggttg acagcctcga aaccgcgttt gtcgcgctat gcgcgacgga
      181 gtgcagactc gatgggtcgc tccgctcgcc gggggccgcg gtccgggccc gccggggcga
      241 agctagtccc agccttcggg cgggaggacg gccttgaccg ccgcatcgca gacttcgtag
      301 gagtagccgc ggcgcgacag catctgctgg atcttcgtcg cctgctcgcg tagcggcttc
      361 gacgcaaccc ggcgcacgta gcgagacgcc aggtcggtcg cctgtgcggt ctcgtccagg
      421 cccccctccg ccagcgcggc ctcggccacc tcgcggtcga tccccttgcc cgtcagctcg
      481 tggagcagag cccggctcga cagcttcttg cgcgtcgacc gttcgcgcac ccactggacc
      541 gcgaaagccc ggtcgtcgac gaggcccagc gtccgcaggc gatcgatcac ggccgagacg
      601 acgcttgctt cgaaccctgc ttcggccagc cgtctcgtga gctcggcctc cgaacgcgcc
      661 cgcgtcgcca gcagccgtcc cgccctcgcc atcgcctcgc ggcgcgctcc gtccaggagg
      721 gctgccgggc ccccgtcgct cggcgactcc gcgacgaggc ccggcatcac ctcttggacc
      781 ggggtcatct aggaagcggc ctccggcacg ggcttctgtg ctggaggccg tccgaggccg
      841 ccctccgcgt cgtcgtcctc gtcggtgccg aggaggccca gcttgtcctt gatcttcagc
      901 tcgatctccg tagcgatgtc cgggttgccg gcgaggaacg acttggcgtt ctcgcgtccc
      961 tgccccagct ggtcgtcgcc gtagatgaac caggcgccgg acttccgcac gatgccgtgg
     1021 tcgatggcga cgtcgaggag gcttccctcc ttcgagatgc cgtagccgta ctggatgtcg
     1081 aactcggcct tcttgaacgg cgcgctgacc ttgttcttca cgaccttcac gcgcaccctc
     1141 gagccgacga cctcggcccc gtccttcagc gagtcgatgc gccgcacgtc catgcggacc
     1201 gacgagtaga acttgagcgc ccggcccccg ggcgtcgtct cgttcgaccc gaacatcacg
     1261 ccgatcttct ccctgatctg gttgatgaag atcaggatcg tcttcgagcg gttgacgctg
     1321 ccggcgagct tgcgcagcgc ctggctcatc cgccgcgcct ggagaccgac gtggctgtca
     1381 cccatctcgc cctcgatctc ggcacggggc acgagggccg ctaccgagtc gacgacgatc
     1441 acgtccatcg cgccggagcg gacgagcgtg tcggcgatct cgagtgcctg ctcgccggtg
     1501 tccggctgtg agcacagcag gttgtcgatg tcgacgccga gcgccttcgc gtagctcggg
     1561 tcgagcgcgt gctccgcgtc gatgaacgac acgagcccgc cgttcttctg cgcctcggcg
     1621 atcacgtgca ggctgaccgt cgtcttgccc gagccctcgg gcccgtagat ctcgacgatc
     1681 ctgcccctcg gcagaccgcc gatcccgagc gcgaggtcga gcgcgagcga ccccgtcgga
     1741 atggcctcga tacccgaggc gatcccggcg tcgccgagcc gcatgatcga ccccttgccg
     1801 tgcggggtct cgatctgggc cagcgccatg tcgagcgccg catcccggcc cttcgcgtcg
     1861 cggctcgtgt cgaccaccgc ggccctggac tccctgattc ctgccacctc acgcctcctt
     1921 gtgccgtggg cccggtcccg ggccctgctc tgcgtgagca agaacctagg gatggggtac
     1981 gacagttata gccgaacata cgttcggggg ccgggattta tccacacgtt acgcacgtgt
     2041 agttacgagg atgtgatcct acttctccag agggccggag gccagcagcg actcagctcg
     2101 gcttggtagc cgagactcga gacgcgacgc aggcccgggg atccgagtgc cccgggcctg
     2161 cgtgcgtcag gtcctttacc tgggtcccat ggcggcggtc gtctcccagc ggtcgtacgc
     2221 cgactggccg aaacgcctga cgaagaagta gtagacctcc ttcaccgcca agcattttcc
     2281 gagattcgac gagtaccacg ggtggcggtt gcggcactcg gattccatgg cccagcggaa
     2341 gtacgtgtct gccgtcgcgc gtgagaccca gtgcgttcgg taggcgtagt cgtggtgggc
     2401 gcaggcgtgc ctgaagtcgt acagcccggg cacgtagtcg ggcgcgtagc tgcagtagtc
     2461 ggtcggagtc tggatatgtg cagctgcggg cgtcgagccc cccacggtca tcgacgacgc
     2521 cagtgaagcg gcgactacga gactgcgaac gagcttcctc ttggatccgc tcttcatcgg
     2581 gtccaccttt ccgatccccc caccggcgac gtgccgccgc cgtccttcgg gtacggaaac
     2641 atccgcgacg aaccacgtaa acggggaagg cgcagtaatg acccggagtt gactcattgt
     2701 cactagttat gtttaccgac gccccggtca cctgacacgt gcgccggcgg ctcggccggg
     2761 gccgccccga agtcagctcg gcttggtggc cgagacccga tcgacgatcc cccacttcag
     2821 ccgctcgagc tcgtcgatcg cgaagccctc gcggcgcagg tggtccagcg gctccctcgt
     2881 gacgtggtcg agcgactgcg cgtccccgcg ccgcaggtcg gcgccgacgc cgagccggac
     2941 ggcacggtcg cgggcccgcg cgagcatctc ctcgctcagc tcgatccccg tgagctcgac
     3001 gccgtcaccg tagaggccga ggttgcggcc ggtcccgacg gcgatctcga gcgtcttacc
     3061 gcgggcgcgc gagcagaccc actcgcggcc cccctggaag agccggcgct cgaagaacga
     3121 gatgcgtttg tcgtagaccc tggctacgcg gtcccagatc gcgttgacgc gtgcggtctc
     3181 ggacccctga gccaccta
//
LOCUS       DASQDO010000586         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11729
            k141_856578, whole genome shotgun sequence.
ACCESSION   DASQDO010000586 DASQDO010000000
VERSION     DASQDO010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945455
            Sequence Read Archive: SRR6517652
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:58:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,202
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,138
            CDSs (with protein)               :: 3,138
            Genes (RNA)                       :: 39
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 1 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 6 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856578"
                     /isolate="SMAG_U11729"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.47 N 105.02 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2358
                     /locus_tag="VEV43_16005"
     CDS             <1..2358
                     /locus_tag="VEV43_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYP25071.1"
                     /translation="QAHLTPGEDFVGFLYPAMDLALASVSLILLHGSGARSASNRLIS
                     VAIALTVATDLVYAGAVADGTYRSGIWYDAGWLLSYALWGVAALHHSAAAAPVPASER
                     RLAMRPSRLLLPSAVVLLAVLFVRELTGRPISVAVALGAGGLVFVLSLVRIRGLLQEL
                     HEHLDEVEDQRRAYRTLMDHASDAIFISRDGRYVDVNDSACALTGYTRAELLELPFGT
                     LSRAAPRTRIDDLLARLERGESIREEMVLTRKDGSTVPIESSARKLPDGRFQSIVRDI
                     SERLESAEALRSSEERFRSAFESPAAGMALVAADGTILQINSTLGQMLGVAPRELEGT
                     PFVALAGDEHRDALQDAFERLAREREGREQLEVELNASSGATTCAEVSLASSDSPSGR
                     TVVAHVVDQTVSRELEDRLRQSQKMEAVGQLAGGVAHDFNNLLSVIVNYTRFAQDAAG
                     PDEGVRQDLEEVLQAATRGERLVRQLLAFSRRERLQPEVVALDDVVSEVADLLRRMLR
                     ESISLELDLAAPGVHVELDRVQLEHVLLNLAVNARDAMPSGGSLRIATAAGGGGLSAH
                     LIVTDTGCGMTPEVRERAFEPFFTTKERGSGTGLGLATVYGAVSGWGGTIDVVSAPGR
                     GTRFDIRMPAAEAAPGPSSPDEGAGPDGRCATVLVAEDEPSLRSIVRRILERHGYRVL
                     MAESSREALEMIEAGVASLDLLLTDVVMPEVSGKDLAERVRSEVPDVAVLYMSGYTDQ
                     IVAQHGLSPNGDSFLQKPFTADDLAGAVSRALARGRRRPQHAATT"
     gene            2376..>2601
                     /locus_tag="VEV43_16010"
     CDS             2376..>2601
                     /locus_tag="VEV43_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYP25072.1"
                     /translation="MDPETTAPVPAGPLLVVDDEEPARRLLARVLRDHGDRCRTAGTL
                     DEARALLDEERFELLITDFDMPGGSGLDLLQ"
BASE COUNT          360 a          936 c          917 g          388 t
ORIGIN      
        1 caggcgcacc tcacgccggg cgaggacttc gtcggcttcc tgtaccccgc gatggacctc
       61 gctctcgcgt ccgtctccct gatcctgctg cacggcagcg gcgcccgcag cgcgtccaac
      121 cggctgatct ccgtcgcgat agcgctcacg gtcgccaccg atctcgtcta tgcgggagcg
      181 gtcgccgacg gtacgtaccg ttcgggcatc tggtacgacg cgggctggct gctgtcgtac
      241 gcgttgtggg gcgtcgccgc cctgcaccac tccgccgccg cggcccccgt gccggcgagc
      301 gagcgccgcc tggcgatgag gccgagccgc ctcctgctcc cgagcgccgt cgttctgctc
      361 gcggtgctgt tcgtgcggga gctcactggc cggccgatct cggtcgcggt cgcgctcggc
      421 gccgggggcc tcgtgttcgt cctgtcgctc gtccgcatcc gggggctcct gcaggagctg
      481 cacgagcacc tcgacgaggt cgaggaccag cgccgggcat atcgcacgct catggaccac
      541 gcatcggacg cgatcttcat ctcgcgcgac ggtcgctacg tcgacgtgaa cgacagcgcg
      601 tgcgcgctca ccggctacac ccgcgccgag ctgctcgagc tcccgttcgg caccctgagc
      661 cgggccgcgc cgcgaacacg catcgacgac ctcctcgcgc gcctcgaacg cggggagtcg
      721 atccgggagg agatggtgct gacgcgcaag gacgggtcga cggtcccgat cgagtccagt
      781 gctcgcaagc tccccgacgg ccggttccag tcgatcgtcc gcgacatctc ggagcgtctg
      841 gagtcggcgg aggcgctgcg ctcgagcgag gagcgcttcc gcagcgcgtt cgaatctccc
      901 gctgcgggga tggcgctcgt ggccgcggac ggaacgatcc tgcagatcaa ctcgacgttg
      961 gggcagatgc tcggggtcgc cccgcgggag ctcgagggga cgccgttcgt ggcgctggcc
     1021 ggtgacgagc accgagacgc cctgcaggac gcgttcgagc ggctggcgcg ggagcgtgag
     1081 gggcgggaac agctggaggt ggagctgaac gcgtcgagcg gcgctacgac gtgtgccgag
     1141 gtctcactcg cctcgtcgga ctcgccgtcg gggcggactg tagtcgccca cgtagtcgac
     1201 cagacggtgt cccgtgagct cgaggacagg cttcgacagt cgcagaagat ggaggcggtc
     1261 ggccagcttg ccggcggcgt cgcgcacgac ttcaacaacc tcctctcggt gatcgtcaac
     1321 tacacgcgct tcgcgcagga cgcggcaggg cccgacgagg gagtgcgaca ggacctggaa
     1381 gaggttttgc aggcggcgac gcgcggcgag aggctcgtgc gccagctcct cgcgttctcg
     1441 cggcgcgagc gcctgcagcc cgaggtggtc gcgttggacg acgtcgtctc ggaggtggcc
     1501 gacctgctcc ggcggatgct gcgcgagagc atctccctcg agctggacct cgccgctccg
     1561 ggggtgcacg tagagctcga ccgagtccag ctcgagcacg tcctgctgaa cctcgcggtc
     1621 aacgcgcgcg acgcgatgcc gtccggcggg tcgctgcgca tcgcaaccgc ggccggcggc
     1681 ggcgggctct ccgcgcactt gatcgtcacc gacaccggat gcgggatgac gccggaggtg
     1741 cgcgagcggg cgttcgagcc gttcttcacc acgaaggagc ggggctccgg caccggcctc
     1801 ggtctcgcca ccgtctacgg cgcggtctcc gggtggggcg gcaccatcga cgtcgtctcg
     1861 gcccccggac gcggcacgcg cttcgacatc cgcatgcccg ccgcggaggc cgctcccggc
     1921 ccttcctctc ccgacgaagg cgccggaccc gacggacgct gcgccaccgt gctggtcgcc
     1981 gaagacgagc cctcgctcag aagcatcgtc cgccggatcc tcgagcgcca cggctatcgc
     2041 gttctcatgg cggagtcctc gcgagaggcc ctcgagatga tcgaggcggg ggttgcgtcg
     2101 ctcgacctcc tcctgaccga cgtcgtcatg ccggaggtct ccgggaagga cctcgccgaa
     2161 cgggtgcgga gtgaggtgcc ggacgtcgca gtcctctaca tgtcgggcta cacggaccag
     2221 atcgtcgccc agcacggcct gtccccgaac ggcgactcgt tcctccagaa gccgttcacg
     2281 gcagacgacc tcgcgggcgc cgtctcgcgt gctctcgctc gaggacgccg acggccgcaa
     2341 cacgccgcca cgacctagga aacggagcaa cgccgatgga ccccgaaacg accgcccccg
     2401 tcccggctgg cccgctgctc gtcgtcgacg acgaggagcc cgcgcgccgg ctcctggcac
     2461 gcgtcctgcg cgatcacggc gaccgctgcc gcaccgcggg gacgctggac gaggcgcggg
     2521 ccctcctcga cgaggagcgc ttcgagctct tgatcaccga tttcgacatg ccgggcgggt
     2581 cgggactcga cctcctgcag c
//
